LOCUS       DADLSB010000001       120006 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.001, whole genome shotgun sequence.
ACCESSION   DADLSB010000001 DADLSB010000000
VERSION     DADLSB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 120006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 120006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..120006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.001"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..326
                     /locus_tag="JN331_000001"
     CDS             <1..326
                     /locus_tag="JN331_000001"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="catalyzes the interconversion of bicarbonate and
                     carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7258250.1"
                     /translation="GLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYN
                     LGHSTIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLK
                     HANHK"
     gene            complement(367..903)
                     /gene="hpt"
                     /locus_tag="JN331_000002"
     CDS             complement(367..903)
                     /gene="hpt"
                     /locus_tag="JN331_000002"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY7258251.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            1109..3499
                     /locus_tag="JN331_000003"
     CDS             1109..3499
                     /locus_tag="JN331_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY7258252.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(3546..5096)
                     /gene="cueO"
                     /locus_tag="JN331_000004"
     CDS             complement(3546..5096)
                     /gene="cueO"
                     /locus_tag="JN331_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAY7258253.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVRLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            5262..5609
                     /locus_tag="JN331_000005"
     CDS             5262..5609
                     /locus_tag="JN331_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY7258254.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            5715..6581
                     /gene="speE"
                     /locus_tag="JN331_000006"
     CDS             5715..6581
                     /gene="speE"
                     /locus_tag="JN331_000006"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY7258255.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            6597..7391
                     /gene="speD"
                     /locus_tag="JN331_000007"
     CDS             6597..7391
                     /gene="speD"
                     /locus_tag="JN331_000007"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY7258256.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(7429..7791)
                     /locus_tag="JN331_000008"
     CDS             complement(7429..7791)
                     /locus_tag="JN331_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY7258257.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(7966..10563)
                     /gene="acnB"
                     /locus_tag="JN331_000009"
     CDS             complement(7966..10563)
                     /gene="acnB"
                     /locus_tag="JN331_000009"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY7258258.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            10918..12771
                     /locus_tag="JN331_000010"
     CDS             10918..12771
                     /locus_tag="JN331_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY7258259.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQASAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(12842..14266)
                     /gene="lpdA"
                     /locus_tag="JN331_000011"
     CDS             complement(12842..14266)
                     /gene="lpdA"
                     /locus_tag="JN331_000011"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY7258260.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(14591..16483)
                     /gene="aceF"
                     /locus_tag="JN331_000012"
     CDS             complement(14591..16483)
                     /gene="aceF"
                     /locus_tag="JN331_000012"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY7258261.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(16498..19161)
                     /gene="aceE"
                     /locus_tag="JN331_000013"
     CDS             complement(16498..19161)
                     /gene="aceE"
                     /locus_tag="JN331_000013"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY7258262.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(19322..20086)
                     /gene="pdhR"
                     /locus_tag="JN331_000014"
     CDS             complement(19322..20086)
                     /gene="pdhR"
                     /locus_tag="JN331_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY7258263.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            20627..22000
                     /gene="aroP"
                     /locus_tag="JN331_000015"
     CDS             20627..22000
                     /gene="aroP"
                     /locus_tag="JN331_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY7258264.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(22043..22897)
                     /gene="ampE"
                     /locus_tag="JN331_000016"
     CDS             complement(22043..22897)
                     /gene="ampE"
                     /locus_tag="JN331_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY7258265.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(22894..23445)
                     /gene="ampD"
                     /locus_tag="JN331_000017"
     CDS             complement(22894..23445)
                     /gene="ampD"
                     /locus_tag="JN331_000017"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY7258266.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            23533..24426
                     /gene="nadC"
                     /locus_tag="JN331_000018"
     CDS             23533..24426
                     /gene="nadC"
                     /locus_tag="JN331_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY7258267.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLTENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            24629..25069
                     /gene="ppdD"
                     /locus_tag="JN331_000019"
     CDS             24629..25069
                     /gene="ppdD"
                     /locus_tag="JN331_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY7258268.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            25079..26464
                     /gene="gspE"
                     /locus_tag="JN331_000020"
     CDS             25079..26464
                     /gene="gspE"
                     /locus_tag="JN331_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAY7258269.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            26454..27656
                     /gene="hofC"
                     /locus_tag="JN331_000021"
     CDS             26454..27656
                     /gene="hofC"
                     /locus_tag="JN331_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY7258270.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(27691..28734)
                     /gene="guaC"
                     /locus_tag="JN331_000022"
     CDS             complement(27691..28734)
                     /gene="guaC"
                     /locus_tag="JN331_000022"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY7258271.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            28959..29579
                     /gene="coaE"
                     /locus_tag="JN331_000023"
     CDS             28959..29579
                     /gene="coaE"
                     /locus_tag="JN331_000023"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY7258272.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            29579..30322
                     /gene="zapD"
                     /locus_tag="JN331_000024"
     CDS             29579..30322
                     /gene="zapD"
                     /locus_tag="JN331_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY7258273.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            30332..30529
                     /gene="yacG"
                     /locus_tag="JN331_000025"
     CDS             30332..30529
                     /gene="yacG"
                     /locus_tag="JN331_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY7258274.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(30629..31018)
                     /gene="mutT"
                     /locus_tag="JN331_000026"
     CDS             complement(30629..31018)
                     /gene="mutT"
                     /locus_tag="JN331_000026"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY7258275.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(31078..33783)
                     /gene="secA"
                     /locus_tag="JN331_000027"
     CDS             complement(31078..33783)
                     /gene="secA"
                     /locus_tag="JN331_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY7258276.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGKRKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(33845..34357)
                     /gene="secM"
                     /locus_tag="JN331_000028"
     CDS             complement(33845..34357)
                     /gene="secM"
                     /locus_tag="JN331_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY7258277.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(34587..35504)
                     /gene="lpxC"
                     /locus_tag="JN331_000029"
     CDS             complement(34587..35504)
                     /gene="lpxC"
                     /locus_tag="JN331_000029"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY7258278.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(35605..36756)
                     /gene="ftsZ"
                     /locus_tag="JN331_000030"
     CDS             complement(35605..36756)
                     /gene="ftsZ"
                     /locus_tag="JN331_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY7258279.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(36817..38079)
                     /gene="ftsA"
                     /locus_tag="JN331_000031"
     CDS             complement(36817..38079)
                     /gene="ftsA"
                     /locus_tag="JN331_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY7258280.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(38076..38906)
                     /gene="ftsQ"
                     /locus_tag="JN331_000032"
     CDS             complement(38076..38906)
                     /gene="ftsQ"
                     /locus_tag="JN331_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY7258281.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(38908..39828)
                     /locus_tag="JN331_000033"
     CDS             complement(38908..39828)
                     /locus_tag="JN331_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase B"
                     /protein_id="HAY7258282.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(39821..41296)
                     /gene="murC"
                     /locus_tag="JN331_000034"
     CDS             complement(39821..41296)
                     /gene="murC"
                     /locus_tag="JN331_000034"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY7258283.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(41350..42417)
                     /gene="murG"
                     /locus_tag="JN331_000035"
     CDS             complement(41350..42417)
                     /gene="murG"
                     /locus_tag="JN331_000035"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY7258284.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(42414..43658)
                     /gene="ftsW"
                     /locus_tag="JN331_000036"
     CDS             complement(42414..43658)
                     /gene="ftsW"
                     /locus_tag="JN331_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY7258285.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(43658..44974)
                     /gene="murD"
                     /locus_tag="JN331_000037"
     CDS             complement(43658..44974)
                     /gene="murD"
                     /locus_tag="JN331_000037"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY7258286.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(44977..46059)
                     /gene="mraY"
                     /locus_tag="JN331_000038"
     CDS             complement(44977..46059)
                     /gene="mraY"
                     /locus_tag="JN331_000038"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY7258287.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(46053..47411)
                     /gene="murF"
                     /locus_tag="JN331_000039"
     CDS             complement(46053..47411)
                     /gene="murF"
                     /locus_tag="JN331_000039"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY7258288.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(47408..48895)
                     /gene="murE"
                     /locus_tag="JN331_000040"
     CDS             complement(47408..48895)
                     /gene="murE"
                     /locus_tag="JN331_000040"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY7258289.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(48882..50648)
                     /locus_tag="JN331_000041"
     CDS             complement(48882..50648)
                     /locus_tag="JN331_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY7258290.1"
                     /translation="MKAAVKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGTIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(50664..51029)
                     /gene="ftsL"
                     /locus_tag="JN331_000042"
     CDS             complement(50664..51029)
                     /gene="ftsL"
                     /locus_tag="JN331_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY7258291.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(51026..51967)
                     /gene="rsmH"
                     /locus_tag="JN331_000043"
     CDS             complement(51026..51967)
                     /gene="rsmH"
                     /locus_tag="JN331_000043"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY7258292.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(51969..52427)
                     /gene="mraZ"
                     /locus_tag="JN331_000044"
     CDS             complement(51969..52427)
                     /gene="mraZ"
                     /locus_tag="JN331_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY7258293.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(53029..54033)
                     /gene="cra"
                     /locus_tag="JN331_000045"
     CDS             complement(53029..54033)
                     /gene="cra"
                     /locus_tag="JN331_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY7258294.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(54115..54201)
                     /locus_tag="JN331_000046"
     CDS             complement(54115..54201)
                     /locus_tag="JN331_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY7258295.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(54213..54704)
                     /gene="ilvN"
                     /locus_tag="JN331_000047"
     CDS             complement(54213..54704)
                     /gene="ilvN"
                     /locus_tag="JN331_000047"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY7258296.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDQTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(54707..56431)
                     /gene="ilvI"
                     /locus_tag="JN331_000048"
     CDS             complement(54707..56431)
                     /gene="ilvI"
                     /locus_tag="JN331_000048"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY7258297.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPFVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(56749..57693)
                     /gene="leuO"
                     /locus_tag="JN331_000049"
     CDS             complement(56749..57693)
                     /gene="leuO"
                     /locus_tag="JN331_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY7258298.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPS
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            58353..58439
                     /gene="leuL"
                     /locus_tag="JN331_000050"
     CDS             58353..58439
                     /gene="leuL"
                     /locus_tag="JN331_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY7258299.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            58532..60103
                     /gene="leuA"
                     /locus_tag="JN331_000051"
     CDS             58532..60103
                     /gene="leuA"
                     /locus_tag="JN331_000051"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY7258300.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            60103..61194
                     /gene="leuB"
                     /locus_tag="JN331_000052"
     CDS             60103..61194
                     /gene="leuB"
                     /locus_tag="JN331_000052"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY7258301.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            61197..62597
                     /gene="leuC"
                     /locus_tag="JN331_000053"
     CDS             61197..62597
                     /gene="leuC"
                     /locus_tag="JN331_000053"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY7258302.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKIMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            62608..63213
                     /gene="leuD"
                     /locus_tag="JN331_000054"
     CDS             62608..63213
                     /gene="leuD"
                     /locus_tag="JN331_000054"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY7258303.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(63262..64440)
                     /gene="setA"
                     /locus_tag="JN331_000055"
     CDS             complement(63262..64440)
                     /gene="setA"
                     /locus_tag="JN331_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY7258304.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(64542..64673)
                     /gene="sgrT"
                     /locus_tag="JN331_000056"
     CDS             complement(64542..64673)
                     /gene="sgrT"
                     /locus_tag="JN331_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY7258305.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            64762..66417
                     /locus_tag="JN331_000057"
     CDS             64762..66417
                     /locus_tag="JN331_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY7258306.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEMPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      66451..66550
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            66581..67564
                     /gene="thiB"
                     /locus_tag="JN331_000058"
     CDS             66581..67564
                     /gene="thiB"
                     /locus_tag="JN331_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY7258307.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWLVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESNLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            67540..69150
                     /gene="thiP"
                     /locus_tag="JN331_000059"
     CDS             67540..69150
                     /gene="thiP"
                     /locus_tag="JN331_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY7258308.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVAIAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            69134..69832
                     /gene="thiQ"
                     /locus_tag="JN331_000060"
     CDS             69134..69832
                     /gene="thiQ"
                     /locus_tag="JN331_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY7258309.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(69946..70710)
                     /locus_tag="JN331_000061"
     CDS             complement(69946..70710)
                     /locus_tag="JN331_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7258310.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(70796..71674)
                     /gene="araC"
                     /locus_tag="JN331_000062"
     CDS             complement(70796..71674)
                     /gene="araC"
                     /locus_tag="JN331_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY7258311.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            72013..73713
                     /gene="araB"
                     /locus_tag="JN331_000063"
     CDS             72013..73713
                     /gene="araB"
                     /locus_tag="JN331_000063"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAY7258312.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            73724..75226
                     /gene="araA"
                     /locus_tag="JN331_000064"
     CDS             73724..75226
                     /gene="araA"
                     /locus_tag="JN331_000064"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY7258313.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYNDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            75426..76121
                     /gene="araD"
                     /locus_tag="JN331_000065"
     CDS             75426..76121
                     /gene="araD"
                     /locus_tag="JN331_000065"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY7258314.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            76196..78547
                     /gene="polB"
                     /locus_tag="JN331_000066"
     CDS             76196..78547
                     /gene="polB"
                     /locus_tag="JN331_000066"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY7258315.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            78712..81618
                     /gene="rapA"
                     /locus_tag="JN331_000067"
     CDS             78712..81618
                     /gene="rapA"
                     /locus_tag="JN331_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY7258316.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            81630..82289
                     /gene="rluA"
                     /locus_tag="JN331_000068"
     CDS             81630..82289
                     /gene="rluA"
                     /locus_tag="JN331_000068"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY7258317.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(82406..83221)
                     /gene="djlA"
                     /locus_tag="JN331_000069"
     CDS             complement(82406..83221)
                     /gene="djlA"
                     /locus_tag="JN331_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY7258318.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            83476..85830
                     /gene="lptD"
                     /locus_tag="JN331_000070"
     CDS             83476..85830
                     /gene="lptD"
                     /locus_tag="JN331_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY7258319.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEALGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            85883..87169
                     /gene="surA"
                     /locus_tag="JN331_000071"
     CDS             85883..87169
                     /gene="surA"
                     /locus_tag="JN331_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY7258320.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDTTLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRGRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQLNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            87169..88158
                     /gene="pdxA"
                     /locus_tag="JN331_000072"
     CDS             87169..88158
                     /gene="pdxA"
                     /locus_tag="JN331_000072"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY7258321.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            88155..88976
                     /gene="rsmA"
                     /locus_tag="JN331_000073"
     CDS             88155..88976
                     /gene="rsmA"
                     /locus_tag="JN331_000073"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY7258322.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDHFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            88979..89356
                     /gene="apaG"
                     /locus_tag="JN331_000074"
     CDS             88979..89356
                     /gene="apaG"
                     /locus_tag="JN331_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY7258323.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVHGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            89363..90205
                     /gene="apaH"
                     /locus_tag="JN331_000075"
     CDS             89363..90205
                     /gene="apaH"
                     /locus_tag="JN331_000075"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY7258324.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(90283..90762)
                     /gene="folA"
                     /locus_tag="JN331_000076"
     CDS             complement(90283..90762)
                     /gene="folA"
                     /locus_tag="JN331_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY7258325.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(90953..92814)
                     /gene="kefC"
                     /locus_tag="JN331_000077"
                     /pseudo
     CDS             complement(90953..92814)
                     /gene="kefC"
                     /locus_tag="JN331_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
     gene            complement(92807..93337)
                     /gene="kefF"
                     /locus_tag="JN331_000078"
     CDS             complement(92807..93337)
                     /gene="kefF"
                     /locus_tag="JN331_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY7258326.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(93385..94779)
                     /locus_tag="JN331_000079"
     CDS             complement(93385..94779)
                     /locus_tag="JN331_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7258327.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(94838..95125)
                     /gene="fixX"
                     /locus_tag="JN331_000080"
     CDS             complement(94838..95125)
                     /gene="fixX"
                     /locus_tag="JN331_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY7258328.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(95122..96408)
                     /locus_tag="JN331_000081"
     CDS             complement(95122..96408)
                     /locus_tag="JN331_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7258329.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEASAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTSENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGNKVGFINLIKDGMKGVTVL"
     gene            complement(96459..97400)
                     /locus_tag="JN331_000082"
     CDS             complement(96459..97400)
                     /locus_tag="JN331_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY7258330.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAC"
     gene            complement(97415..98185)
                     /locus_tag="JN331_000083"
     CDS             complement(97415..98185)
                     /locus_tag="JN331_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY7258331.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            98600..100114
                     /gene="caiT"
                     /locus_tag="JN331_000084"
     CDS             98600..100114
                     /gene="caiT"
                     /locus_tag="JN331_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HAY7258332.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWMFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            100145..101287
                     /gene="caiA"
                     /locus_tag="JN331_000085"
     CDS             100145..101287
                     /gene="caiA"
                     /locus_tag="JN331_000085"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY7258333.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            101416..102633
                     /gene="caiB"
                     /locus_tag="JN331_000086"
                     /pseudo
     CDS             101416..102633
                     /gene="caiB"
                     /locus_tag="JN331_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            102707..104260
                     /gene="caiC"
                     /locus_tag="JN331_000087"
     CDS             102707..104260
                     /gene="caiC"
                     /locus_tag="JN331_000087"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY7258334.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDHPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            104369..105154
                     /gene="caiD"
                     /locus_tag="JN331_000088"
     CDS             104369..105154
                     /gene="caiD"
                     /locus_tag="JN331_000088"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY7258335.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            105160..105750
                     /gene="caiE"
                     /locus_tag="JN331_000089"
     CDS             105160..105750
                     /gene="caiE"
                     /locus_tag="JN331_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY7258336.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(105836..106231)
                     /gene="caiF"
                     /locus_tag="JN331_000090"
     CDS             complement(105836..106231)
                     /gene="caiF"
                     /locus_tag="JN331_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY7258337.1"
                     /translation="MCEGYVEKLLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(106493..109714)
                     /gene="carB"
                     /locus_tag="JN331_000091"
     CDS             complement(106493..109714)
                     /gene="carB"
                     /locus_tag="JN331_000091"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY7258338.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(109732..110880)
                     /gene="carA"
                     /locus_tag="JN331_000092"
     CDS             complement(109732..110880)
                     /gene="carA"
                     /locus_tag="JN331_000092"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY7258339.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFLGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(111336..112157)
                     /gene="dapB"
                     /locus_tag="JN331_000093"
     CDS             complement(111336..112157)
                     /gene="dapB"
                     /locus_tag="JN331_000093"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY7258340.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(112324..113238)
                     /gene="rihC"
                     /locus_tag="JN331_000094"
     CDS             complement(112324..113238)
                     /gene="rihC"
                     /locus_tag="JN331_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY7258341.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(113304..114254)
                     /gene="ispH"
                     /locus_tag="JN331_000095"
                     /gene_synonym="lytB"
     CDS             complement(113304..114254)
                     /gene="ispH"
                     /locus_tag="JN331_000095"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY7258342.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(114256..114705)
                     /gene="fkpB"
                     /locus_tag="JN331_000096"
     CDS             complement(114256..114705)
                     /gene="fkpB"
                     /locus_tag="JN331_000096"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7258343.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(114792..115286)
                     /gene="lspA"
                     /locus_tag="JN331_000097"
     CDS             complement(114792..115286)
                     /gene="lspA"
                     /locus_tag="JN331_000097"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY7258344.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(115286..118102)
                     /gene="ileS"
                     /locus_tag="JN331_000098"
     CDS             complement(115286..118102)
                     /gene="ileS"
                     /locus_tag="JN331_000098"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY7258345.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYVEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(118145..119086)
                     /gene="ribF"
                     /locus_tag="JN331_000099"
     CDS             complement(118145..119086)
                     /gene="ribF"
                     /locus_tag="JN331_000099"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY7258346.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(119094..119312)
                     /locus_tag="JN331_000100"
     CDS             complement(119094..119312)
                     /locus_tag="JN331_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY7258347.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            119415..119678
                     /gene="rpsT"
                     /locus_tag="JN331_000101"
     CDS             119415..119678
                     /gene="rpsT"
                     /locus_tag="JN331_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY7258348.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        28787 a        33041 c        30356 g        27822 t
ORIGIN      
        1 tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa catagctcat
       61 tgctcggcga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg aacgtcatgg
      121 aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa cgcgggcaga
      181 aagttaccat tcacggctgg gcctacggca ttcacgacgg cttgctgcgt gatctggatg
      241 ttaccgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt tccaacctca
      301 agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca tccggcaact
      361 tcacacttac tcgtccagca gaatcacttt gccaatatac ggcagatgac ggtaacgctg
      421 tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac cgataaattc
      481 aaccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa tcgccaacga
      541 cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc ccgagtcgat
      601 gatatcttca acaatcagca cgtccttgcc acggatatcc tcatccagat ctttgaggat
      661 tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca taaagtcgac
      721 ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg agccacgcag
      781 cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa tctgacgacc
      841 cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta cagtatgttt
      901 catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt tttggcaccg
      961 caacaaagcg caagacacgc agtataccag taaaactatt cattcgcgga atctgttaat
     1021 aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct ataattaatt gctactaaaa
     1081 tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacaggct cgcgacgatt
     1141 actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac tcattggcgg
     1201 aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc ttgtgatgct
     1261 cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg ctttggctat acgcagccct
     1321 gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct gggcgctgac
     1381 tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt ttgtctggcg
     1441 tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtcgcac tgctgattag
     1501 cggtggtatc ctgacctggg ccggatttaa cgatccgcag gagatcaacg gcaccttaag
     1561 cgccgatgcc acacctgccg aagctatctc ccccgttgcc gatcaggact ggcctgcgta
     1621 tggccgcaat caggaaggtc aacgcttttc accactgaag caaattcacg ccgataacgt
     1681 ccacaagctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc cgaacgatcc
     1741 gggtgaaatc accaatgaag tgacgccgat taaagtgggc gatacccttt acctgtgtac
     1801 cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc aaagagaaat ggcattacga
     1861 tcctgagctg aaaaccaacg agtctttcca gcacgtaacc tgccgtggcg tctcttatca
     1921 tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc gtcgtatcat
     1981 tcttccagtg aatgatggtc gcctgattgc gattaacgct gaaaacggca aactgtgcga
     2041 aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca ccaaaccggg
     2101 tctgtatgag ccgacttctc cgccaattat caccgataaa accatcgtga tggccggttc
     2161 agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt ttgatgtcaa
     2221 caccggtgag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg caatcccgtc
     2281 tgacgaacac acctttacct ttaactcacc aaactcgtgg gcaccagcgg cctatgacgc
     2341 gaagctggat ctggtctatc tgccgatggg cgtgaccacg ccggatatct ggggcggtaa
     2401 ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg ccactaccgg
     2461 gaaactggcg tggagctacc agaccgttca ccacgacctg tgggacatgg atcttccggc
     2521 acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccgatta tttacgctcc
     2581 ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat ggcgaactgg tggttccggc
     2641 cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc ctactcaacc
     2701 attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata tgtggggagc
     2761 caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct atgaaggcat
     2821 tttcacgccg ccatctgaac agggtacgct ggtcttcccg ggtaacctgg ggatgttcga
     2881 atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc caatggcact
     2941 gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac ccgatggagc agccgaaaga
     3001 tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac cgtatggtgt
     3061 cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag catggggtta
     3121 tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta ttggtacgcc
     3181 gcaggacagt atgccgttcc cgatgccggt tccggtgccg ttcaatatgg gtatgccgat
     3241 gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta cggcagataa
     3301 ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc gtttaccggc
     3361 gggtggtcag gcaacaccga tgacctatga agtgaatggc aagcagtacg tggtgatctc
     3421 cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg cttatgccct
     3481 gccggatgat gtgaaataac cataatgccc ggagagatcc gggcatattt ccgaatacgg
     3541 tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag cagatggcag
     3601 tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa acttcaccag cacttcactg
     3661 acattgcctt ctaccttaac ggtatctttc cagcccgcgc gatgagccgc tggcggtttg
     3721 ccattttctg acaagatacg gaactgcgtg ccgtgaatat ggaacggatg cagcatcatg
     3781 tcgcccacgc cagagataac ccaacgttcg tactgccctt tcgccgccgc aaacatcggc
     3841 ttattcatat caaacgcctg accgttgatt ttattggcat ggtggaaatc gaacttcccg
     3901 ccgtggttca tatgattcat attgccgtgc cccatatggc ccatcatctg gctgtgatcc
     3961 attccggcca tcgcctgatc gccatatttc tccattagca tctgcatccc catcatatcg
     4021 agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag cgaaggtaac
     4081 gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg cactaatagc aatcggctga
     4141 atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg ccatccccat ctggctgacc
     4201 ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac ttcaaaacgc
     4261 tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt caggtagcag accaccgtcg
     4321 ctggcaatca catacagcgg gcgattgtcg ctggtggcga aattgagcga gcgggcatta
     4381 cagccattga gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg tgggtagatt
     4441 gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg cggcggtcat cacatccagt
     4501 tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct gaacgatcac cggaacatca
     4561 tcgatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc aatcaccacc
     4621 agaccagcca gccccatcgc cacctgtcgc ccggtcttgc catgttgatg tggatggaac
     4681 cagcaggtag cggcaggttg atcaacgttc aacctcaccg agcgcttgcc acctggcgga
     4741 ataattccct gtgggccgcc gtcgacgtca cccggtactt ccagcccgtg ccagtgtaac
     4801 gtcgtctctt ccgccagttg gttgtagata tcaaccgtta ccgctttgcc gcgctgtaat
     4861 ttcaccgccg gccccagcag attgccgtta tagccccagg tagttgcagt tttctcgcca
     4921 aaggtggact gacctgcgcc aatagttaac tgaatgcgat tacgggcatc ggtcgtgagc
     4981 aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga ctgcacggct ccacagtggc
     5041 aaggctgaag ccacacccag tgcgacggaa tatttcaaga aatcacgacg ttgcatagtt
     5101 atttccttat tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc cttcccctta
     5161 cgggaaggtc aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc ctcgctcgca
     5221 gtgtgctaac gtttatcttc tttaagcccc tggtagaagc aatgaagacg tttttcagaa
     5281 cagtgttatt cggcagcctg atggccgttt gcgcaaacag ttacgcgctc agcgagtctg
     5341 aagccgaaga tatggccgat ttaacggcag tttttgtctt tctgaagaac gattgtggtt
     5401 accagaactt acctaacggg caaattcgtc gcgcactggt ctttttcgcc cagcaaaacc
     5461 agtgggatct cagtaattac gacaccttcg acatgaaagc tctcggtgaa gacagctacc
     5521 gcgatctcag cggcattggc attcccgtcg ctaaaaaatg caaagccctg gcccgcgatt
     5581 ccttaagcct gcttgcctac gtcaaataat ccctgatacc tttttgtaga aatattgacc
     5641 gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt tttttacggg tgttaacaaa
     5701 ggaggtatca acccatggcc gaaaaaaaac agtggcatga aacgctacac gaccagtttg
     5761 ggcagtactt tgcggtagat aacgttctgt atcatgaaaa gaccgatcac caggatctga
     5821 tcatttttga gaacgctgca tttggtcgcg taatggcgct ggatggcgta gtacaaacca
     5881 ccgagcgcga cgagtttatc tatcatgaga tgatgaccca tgttccgcta ctggcccacg
     5941 gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg tgccatgctg cgtgaagtaa
     6001 cccgacataa aaacgttgag tcaatcacga tggtggaaat cgatgcgggt gtcgtgtcgt
     6061 tctgccgtca gtatctgccc aaccataacg ccggtagcta cgacgatccg cgctttcaac
     6121 tagtgatcga cgatggcgtc aatttcgtta atcaaaccag ccagaccttt gatgtcatta
     6181 tctccgactg caccgatcct atcggtcccg gcgaaagcct gttcacctca gcattttatg
     6241 aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt cgcacaaaac ggcgtctgct
     6301 ttttacagca ggaagaagcc atcgacagcc atcgcaaact cagccattac ttcagcgacg
     6361 ttggctttta tcaggcggcg atcccgacct attacggcgg tatcatgact tttgcatggg
     6421 cgacagataa cgacgcctta cgccatctct caaccgaaat tattcaggcg cgttttctcg
     6481 cttctggcct gaaatgccgt tattacaatc cggcaatcca tacagcagct tttgccttac
     6541 ctcagtatct gcaagacgca ctggcttcac agccgtccta aggagaagat aagaaattga
     6601 aaaaactgaa actgcatggc tttaataatc tgaccaaaag tctgagtttt tgtatttacg
     6661 atatctgcta cgccaaaact gccgaagagc gcgacggtta tattgcttat atcgatgaac
     6721 tctataatgc caaccgtctg accgaaatcc tgtcagaaac ctgttccatt atcggggcca
     6781 atattcttaa catcgcccgc caggattacg aaccacaggg tgccagcgtc actattctgg
     6841 tgagtgaaga accggttgac ccgaaactca tcgacaaaac agaacacccc ggcccactgc
     6901 cagaaacggt ggttgctcat ctcgataaaa gtcatatttg cgtacatacc tacccggaaa
     6961 gtcatcctga aggcggttta tgtaccttcc gcgccgatat tgaagtctct acctgcggcg
     7021 tgatttctcc gctgaaggcg ctgaattacc tgatccacca gcttgagtcc gatatcgtaa
     7081 ccattgatta tcgcgtgcgc ggttttactc gcgacattaa cggtatgaag cactttatcg
     7141 accatgagat taattcgatt cagaacttta tgtctgacga tatgaaggcg ctgtatgaca
     7201 tggtggatgt aaacgtctat caggaaaata tcttccatac caagatgttg cttaaagagt
     7261 tcgaccttaa gcactacatg ttccacacca aaccggaaga tttaaccgac agcgagcgcc
     7321 aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat ttattacggg cgcaatatgc
     7381 cagctgttta acggctctgg cggagctccc aggctccgcc agatttattt acttctgctg
     7441 cacgaaattg cggtaagccg ccacgacctg cagaaaatcc tcaacgccgc atagcgacag
     7501 gctttcttcg tcgtagtagt tcatcccctc ttccatttca tcgccagcga attccagttg
     7561 attggcgcga accatcacct cttcagcgtc catccacagg gtgtattcat gccctgcccg
     7621 ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct tgttccactt catcaagcaa
     7681 ggccaggttt tctttcacct cttcattaaa ccagtgcccg accacttcat gccccatgga
     7741 catacgcacc tttaccactc cggtaatatc gcgcagaaat tcgtaatcca tagtgtgttc
     7801 ctctgctccc ggcaatgcgc cgaaatcgca tcgcgccatt gatgtaatta tcgcagcctt
     7861 accggagaaa aacagcggaa cggcgatggt ttagaaataa aaaatgcccg ggaatctcat
     7921 tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt acttattaca ccgcagtctg
     7981 gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt tcagataacg
     8041 ataagtatca acggcagttt tatctacttg cgcaacgtag gtctggtact cttccggcgt
     8101 cggcagtttg ccaatcagcg ccgcaacagc cgccagttct gcagaagcca ggaagacatt
     8161 cgcgccagta cccaaacggt tcgggaagtt acgggtagag gtggaaacca ccgtcgcacc
     8221 gtctgctaca cgcgcctggt tacccataca cagggaacag cccgggatct cgatacgagc
     8281 cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc agttgcgcgg cgtccatacg
     8341 ggttggcggt gccacccaca ggcgggtcgg caactgacct ttgtgcgcat ccagcagttt
     8401 accagcggca cggaagtgac cgatgttagt catgcaagaa ccgataaata cttcgtcgat
     8461 cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc ggatcgttcg gagcacacag
     8521 gattggctct ttaatatccg ccagatcgat gtcgatcact gccgcgtatt ccgcatctgc
     8581 atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa tacggcgttc
     8641 cagggtacga cgatcgccgt aaccttccgc gatcatccac ttcagcagga cgatgttgga
     8701 gttcaggtat tcgatgatcg gttctttgtt cagcttgata gtacaaccag cggcagaacg
     8761 ctcggcggac gcatcggtta gctcaaaggc ctgctcaact ttcagatccg gcagaccttc
     8821 aatttccaga atgcgaccag agaagatgtt tttcttgcct ttcttctcaa cggtcagcag
     8881 accttgtttg atcgcataca gcggaatggc gtgtaccaga tcgcgcaggg tgatgcccgg
     8941 ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg gcattacgcc
     9001 agttgcggca gcaaacgcca ccagaccaga acccgccggg aaagagatac cgatcgggaa
     9061 acgggtatgg gagtcaccac cggtaccgac ggtatccggc agcagcatac ggttcagcca
     9121 ggagtgaatg acgccgtcac ccggacgcag cgacacaccg ccacggttca taatgaagtc
     9181 cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg tgtggcagaa
     9241 agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca gttcatcacg
     9301 ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc attttcggtt cgcagtacgc
     9361 gcccggacga atgcctttca cgccacaggc acggcctacc attttctgcg ccagcgagaa
     9421 gccgcgatcg ctctcagcga catctttcgc ctgacggaac acatcactgt gcggcagacc
     9481 aagtgcttca cgcgctttgg tggtcaggcc acgcccgata atcagcggaa tacggccgcc
     9541 agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca gcagttcgcc
     9601 tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt cgcccatgtt
     9661 caggttagag acgttgactt cgattggcag tgcacccgcg tcttccatcg tgttaaagaa
     9721 gatgggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg gcacatgtgg
     9781 aatatcatcg cccataaacc acagaacgga gttcgtggcg gatttacgcg atgaacccgt
     9841 acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag cttcaatctg
     9901 tttgatcggg ccaacaacac ctggctggtc tggctcaata ccttcacggg cgtttttcag
     9961 catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg ccggagaaag
    10021 gtcatcggtg ttggtttcgc cagtgacttt aaagacggta acggtcagtt tttcagccag
    10081 cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcattacct gcttcgcata
    10141 ttcgttgcct gctttcgctt tctcttctac gtcatagaag ttatcgaaca tcagcagcgt
    10201 gtgagaaagt gctttggcag caataggtgc cagtttggca tcatccagcg cgtcgatcag
    10261 cggatgaatg ttgtaaccac cctgcatggt gcccagcagt tcgatagctt tttccggagt
    10321 cagcagaggg gatttagctt cgcctttagc gatagcagcc aggaagcctg cttttacata
    10381 ggcggcttca tcgacgcctg ggggaacacg gttggttaac agatctaaca ggaattcttc
    10441 ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg gccatttggt ttgcatccag
    10501 gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt attcttctag
    10561 cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct tcacgctcct
    10621 gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat agcaggattt
    10681 tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga atcgttaagg
    10741 atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt gataatcacc
    10801 tttctcaacg aatcccaaga ttagtcaaaa tttaaactac cgcctcttta tactcggatt
    10861 cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat ccgaattatg
    10921 aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc cgggctttgt
    10981 gctgcctctg ccgggctata tataaaaagc cgcacagtgg aagcgcctgt agaaccgcaa
    11041 tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc ttcctgcaac ggtttccgca
    11101 cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca ctgcacaaat agatcaatgg
    11161 gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc aggtgctgat ggcatcaacc
    11221 tatccgacaa acgttgctca agcagtgcaa tggtcgcacg ataatccact taaacaaggc
    11281 gatgctgcta ttcaggcggt atctgaccag ccgtgggacg ccagcgttaa atcactggtg
    11341 gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca aaacctgggc
    11401 gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt gcggcaactg
    11461 gcgcaacaaa ccggctcgct gaagtcatca accgaacaga aagttattac cacaacgaag
    11521 aaagctgtac cggtaaaaca gacagtcacg gcccccgtca taccatccaa taccgtttta
    11581 actgccaacc ccgtcattac agagccggca acaaccgtca tttccattga gcccgccaat
    11641 cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa ctgggccaat
    11701 actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttgt tgacagcttt
    11761 gtgcgcggat tcggctatag catgggcgtt gctaccacgt acgcactatt cagcagcatc
    11821 gactgggatg acgacgatca tgaccatcat catcatgacg atgatgatta tcatcaccac
    11881 gatggcggtc atcgtgacag taatggctgg caacacaacg gcgacaacat caatatcgac
    11941 gtcaacaatt tcaaccgtat caccggtgag catcttactg ataagaatat ggcatggcgg
    12001 cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc aaagcggttt
    12061 catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc aacacgcgac
    12121 agccagcgtc aggcggcagc aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt
    12181 acacgagata cccaacgtca ggcatcggca cagcggttta atgaagctga acactatggg
    12241 agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca acaaaaggac
    12301 gccgctcgtc agcgttatca gtcggcctct cctgagcagc gccaggcagt ccgcgagaaa
    12361 atgcagacta acccgcagat ccagcagcga agggaggcgg cgcgccagcg tattcagtcc
    12421 gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc agactaaccc gcagaaccag
    12481 cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa ctcccgaaca gcgccaggtg
    12541 tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag
    12601 cgtatacaat cggcatcacc tgaacaacgt caggtttttc gggagaaagt tcaggagagc
    12661 cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca
    12721 gcagtacgcg aacgtctctc tgaacgtgga gcaagaagac tggaaaggta aattgcagac
    12781 gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaaa
    12841 attacttctt cttcgctttc gggttcggca ggtcggtaat gctaccttcg aacacttctg
    12901 ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg gatggtcagt gcgatgtctt
    12961 cagcatcaca acccatttcg attgccaggc cgatttcacc cagcagctcg ccgccgttgg
    13021 taccaacaat cgcaccaccg atcacacggt gagattcttt gtcgaaaatc agcttggtca
    13081 taccgtctgc gcagtcagaa gcgatagcac gaccagaagc agcccacggg aaggtggcgg
    13141 tttcatagct gatgcctttc tctttcgctt ctttctcagt cagacctacc catgcaactt
    13201 ctggttcggt ataggcgatg gacgggataa ctttcggatc gaaatagtgt ttcttaccgg
    13261 cgataacttc agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc atcggctgac
    13321 cgacgatatc gccgatagca aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc
    13381 ggatgaaacc acggtcgtca acttccacgc cagctttgcc tgcgtcgagg tttttaccgt
    13441 tcggcacacg accaatcgct accagcacgg cgtcgtaacg ctgcggttca gctggtgctt
    13501 ttttgccttc catcgtcaca taaataccgt cttctttcgc ttcaacggcg gtaactttgg
    13561 tttccagcat caggttgaat ttcttgctga tacgcttggt gaagacttta acgatgtctt
    13621 tgtcagctgc cgggataacc tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg
    13681 cgtggtaaac ggtgcccatt tccagaccga tgataccgcc acccattacc agcaggcgtt
    13741 ctggtacttc tttcagttcc agcgcgtcag tggagtccca gatacgcgga tcttcatgcg
    13801 gaataaacgg cagttggatt gggcgagaac ccgctgcaat gatcgcattg tcgaagttga
    13861 tcacggtttt gccgttctca ccttcaactt ccagggtgtt agccccggtg aatttaccca
    13921 gaccgttgac cactttgact ttgcggcctt tcgccatacc agccagacca ccggtcagct
    13981 gattgatcac tttctctttc caggtacgaa tcttgtcaat atcggttttc ggttcgccga
    14041 agacgatacc gtgttcagcc agcgctttgg cttcttcgat aacttttgct acgtgcagca
    14101 gtgctttaga agggatacag ccgacgttca ggcaaacacc gccaagggtg ttgtaacgtt
    14161 ctacgattac ggtttccaga cctaaatcag cgcaacggaa ggcagcggag taacctgcgg
    14221 ggcctgcccc aagtaccacg acctgagttt tgatttcagt actcatcatg acctctatat
    14281 atttatctcc ggcggtcata cccgtcgtct ttcaggccgc agcggcgtta gcttcacgcc
    14341 ctcaccccag ccacttactt atgtaagctc ctggggattc tggcgcttgc taccttgctg
    14401 cacccagaaa tccatagggt atgtgaagta ataccctaac caccaccggg tcgttctatc
    14461 cgtcggtatt ttacaaaatt gttaacaatt tttaaacaac aaacggcaac cgatttgtct
    14521 attcgctaat aacctcaata cattcatgag attaccagaa aaaagccggc cgttgggccg
    14581 gctcttttac ttacatcacc agacggcgaa tgtcagacag cgtgttgtta atgatggtaa
    14641 tgaaacgggc accatcagca ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca
    14701 gcatcagacg cggcacgaac tctttaccat tccacaccgg ctccatcgcg gacttggaaa
    14761 cgccgaggat agccacttcc ggcgcgttca caatcggcgc gaagtgggta gtacccaggc
    14821 cgccgatgct ggagatggtg aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt
    14881 cacgcgcttt cttagaaata gtcatcagct cgcgagacag ctcgatgatg cctttcttgt
    14941 tgacgtcttt gaataccgga acaaccagac cgttcggggt atccaccgcc acaccgatgt
    15001 tgatgtattt cttcagggtc agacgctgac cgtcttccga cagcgaacta ttgaagcgag
    15061 gcatctgctc aagagctgca gcaacggctt tcatgatgaa gacaaccggg gtgatcttca
    15121 catccagctt acgtttcgcc gcttcttcgt tctgctgttt acggaacgct tccagctcgg
    15181 tgatatcggt tttgtcgaag tgggtaacat gcgggatcat cacccagtta cggctcaggt
    15241 tagcaccaga aattttctgg atacggccca gttccacttc ttcgatttcg ccaaacttgc
    15301 tgaagtccac cttcggccac ggcagcatgc cagggatacc accgccagtc gccgccggag
    15361 ctgcttctgc acgtttgata gcttctttca cgtaagcctg aacgtcttcg cgcaggatac
    15421 gacctttacg gccagtgccc ttcactttcg ccaggttaac gccaaactcg cgtgccagac
    15481 ggcggatcag cggagtcgcg tgaacgtaag cgtcgttttc agcaaattca gatttgcctt
    15541 ccgctttcgc agccggagct gctgccggag cttcagcttt tgctgccgga gccggcgctg
    15601 ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg aagatcataa
    15661 tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc acgacgcctg
    15721 caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc agtgactgtt
    15781 cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact tcgtcaccgc
    15841 cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc gctgccggag
    15901 ccgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact tcgaagacca
    15961 taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct ttcacggtac
    16021 ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg atcagcgact
    16081 gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca acttcgtcgc
    16141 tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct gctgccggag
    16201 ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg tcggcggaat
    16261 cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact ttgatctctt
    16321 taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct tctacggtga
    16381 tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg gtgatttcaa
    16441 cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt ttacctctta
    16501 cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg cgtcagcaac
    16561 cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa ccacgacata
    16621 agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac gaccgaagcc
    16681 atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct gctcagcgaa
    16741 cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct gagcgatata
    16801 cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat cctgaccatc
    16861 acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc cgtaatcttt
    16921 cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc ccagcagctg
    16981 aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac ggataccttc
    17041 ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag tgatgtagta
    17101 gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca taatgacagc
    17161 aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca gcgactgaat
    17221 gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac gaccggaagt
    17281 accgccgatc aggaagccac gcgcttgctg gtcgccagcc gcccagcaca gatcgccgat
    17341 acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca gattgttcgt
    17401 gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt tgatcccttc
    17461 ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc ggtcctgcgg
    17521 ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac cttccatacc
    17581 gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg acttgttctt
    17641 cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagatctctt tgctctgctc
    17701 ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg tgaagttcgg
    17761 ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat aggtatgctc
    17821 ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat cagacaccgg
    17881 cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct taacctggtg
    17941 cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg tatgagcaag
    18001 gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt agattttctt
    18061 cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt ctgcaaccag
    18121 tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac catctttcga
    18181 tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca gtttaccgct
    18241 ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga tcacgttcca
    18301 gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac cggtgaccgg
    18361 gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca gtttttcacg
    18421 ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt cgccgaggaa
    18481 cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat atttcaggaa
    18541 tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg ggaactgcca
    18601 gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt gaacttcctg
    18661 acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac gggcgtaaac
    18721 gcccggggag atgtggccct ggaagtaaac caagtcgccg ccatcctgct cgttgcgtgc
    18781 acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga aggacgccat
    18841 gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga tggcgttcca
    18901 gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact ccggttgttc
    18961 ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta cgtttacacc
    19021 gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct caacaccttc
    19081 ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg gatccacgtc
    19141 atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt gagttttctg
    19201 gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta cgtttagttg
    19261 ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac cagaaaaatc
    19321 actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc tcttcacggc
    19381 ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct tcgcgcgctt
    19441 cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta ctcaccagcg
    19501 gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc tgggccaaca
    19561 tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct tcggtgacgg
    19621 caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac tgctgcgcca
    19681 gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta ctacgcagcg
    19741 cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag tcatactgtg
    19801 attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc cataggctgc
    19861 tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct tcgagacgtt
    19921 gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt tcgcgttccg
    19981 gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc agttgctgct
    20041 caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt tcctgtctta
    20101 agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct aaagtaacaa
    20161 agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg cacattttaa
    20221 caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga ctttacgtag
    20281 gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg attcacttac
    20341 caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca tattgagagg
    20401 ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca accatgcata
    20461 aagcgggtgc attcgctgcc gtataccatt attcttgatc tgacggaagt ctttttgtaa
    20521 caattcaaac ttccttgatg taaataaatc aatacaacaa acggaattgc aaacttacac
    20581 acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga tggaaggtca
    20641 acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc ttatcgcgct
    20701 gggtggcgcg atagggacag ggctattcct gggtagcgcc tccgtaatac agtccgcagg
    20761 gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga tcatgcgtca
    20821 gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact ttgcttataa
    20881 atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac tgtacgtttt
    20941 agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt atccggaaat
    21001 cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca acctgaccaa
    21061 cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta ttgcggtagt
    21121 agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc cgcaggcgag
    21181 cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg gactggtgat
    21241 gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga tcaccgcagc
    21301 agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta tctaccgcat
    21361 cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt ggacccgcgt
    21421 taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata cctttgtggc
    21481 gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca gctgcgtata
    21541 ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa aagcgctggc
    21601 gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc tggttacagc
    21661 attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt taatggcact
    21721 ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata tgaagttccg
    21781 tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt atccgctggg
    21841 taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga tgaccccagg
    21901 aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta tcggctatct
    21961 gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg ccctcacccg
    22021 tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt agcgcaatca
    22081 ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc ggcgtctcca
    22141 ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt aatacctgat
    22201 attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct ttctcaccat
    22261 gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc agtacatgaa
    22321 gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc gccagccagt
    22381 attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag gttcccccca
    22441 caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc aatgcatttt
    22501 gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga atcatggtga
    22561 gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct gtcagataag
    22621 catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc agcagccaca
    22681 ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca
    22741 cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt ttcacccgcc
    22801 gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta aacaggcgct
    22861 cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc tgaccagcgc
    22921 acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg gcgcaatatc
    22981 acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg cccgcgtaac
    23041 cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc cttcaagctc
    23101 aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga ctcccgcatg
    23161 ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc gaatcaaaca
    23221 gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat
    23281 agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg gcaggctaat
    23341 attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt aatgtggtga
    23401 gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg atctccttat
    23461 atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc gttagctatc
    23521 tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga cgagctgctg
    23581 gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga agatttaggc
    23641 ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taacggaaaa ttctcgctct
    23701 catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg ggttgaagag
    23761 gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga tgacggcgat
    23821 gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg
    23881 ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag taaggtacgc
    23941 cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg caaaacctta
    24001 cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt
    24061 ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc cggctcagtg
    24121 cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga agtcgaagta
    24181 gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat catgctggat
    24241 aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg
    24301 gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac gggcgtggac
    24361 tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc aatgcgtttt
    24421 cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc cttccccaac
    24481 tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa aaaacctcgg
    24541 aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt tgccaaagta
    24601 gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta cacttatcga
    24661 actgatggtg gttattggca tcattgccat tttaagcgcc attggtattc ccgcttatca
    24721 aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc cttaccgtac
    24781 cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg gtggcagcaa
    24841 tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg caaagggcgt
    24901 ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga cgccaggttg
    24961 ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa gtgacagcgc
    25021 attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag gagcggcaat
    25081 gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc tggatgccag
    25141 cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc tggacgcatt
    25201 gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac aaatggaagg
    25261 tcacgccagt cgcacacaac agacattgcc cgtagctgtt caggagaagc atcagcccaa
    25321 agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt ctgatattca
    25381 tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc
    25441 tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa aagtgctggg
    25501 aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg tcgaactggc
    25561 aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg aaaaggtggt
    25621 attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg gaatgcagcc
    25681 gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg tgctggtaac
    25741 cggccctaca ggcagcggca aaacggtcac gctttatagt gccctgcaaa cgctgaatac
    25801 cgctgacatt aatatttgta gcgtcgaaga tccggttgag atccccatag ccggactaaa
    25861 ccagacgcaa atccatccgc gtgccgggct cacctttcag ggcgttttgc gtgcgttatt
    25921 gcgccaggat cctgacgtca tcatgatcgg agagatccgc gatggcgaaa cggcagaaat
    25981 tgccattaaa gccgcgcaaa ccggtcacct ggtgttgtcc acgctacaca ccaattccac
    26041 cactgaaacg ctggtacgtt tacagcaaat gggggtcgcc cgctggatgc tatcatcggc
    26101 gcttacgctg gtaatagccc agcgtctggt acgtaaactt tgcccacatt gtcgccgaca
    26161 gcaaggggag cccatccata ttccagacac tgtatggccg tcgccgctgc cccactggca
    26221 ggcacccggt tgtgtacatt gctaccacgg tttttatggt cgtaccgcct tgtttgaagt
    26281 cctgcccatt acaccagcca tacgccagct tatttccgct aatactgaag ttgaatcgct
    26341 ggaaacgcac gcccgacagg cgggtatgcg tacgcttttt gaaaacggct gcctggccgt
    26401 ggagcaaggc ttaaccacct ttgaagagtt aatccgcgta ctggggatgc cgcatggcaa
    26461 gtaagcaact ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc
    26521 tatgggcaga gagccgtact ttactgctta tggcactaca gcaacagatg gttaccccac
    26581 taagcctgaa gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag
    26641 tcattcatca actggcgacg ctacttaaag ccgggttaac gctttctgaa gggctggctc
    26701 tgctggcgga acagcatccc agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg
    26761 atctcgaaca gggcattgct ttttccaatg ccttattacc ctggtcagag gcatttccgc
    26821 cgctctatca ggcgatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct
    26881 ttgaactggc gcgtcagcaa aaatcccagc gtcagttgac cgacaaagtg aaatcagcgt
    26941 tgcattatcc catcatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt
    27001 ttgttctgcc ggagtttgcc gctatctata agaccttcaa caccccacta ccggcactaa
    27061 cgcaggggat catgacgctg gcagacttta gtggcgaatg gggctggctg ctggtgttgt
    27121 tcggctttct gctggcgata gccaataagt tgctgatgcg ccgcccgacc tggcttatcg
    27181 cgcggcagaa attgctgtta cgcatcccga ttatgggttc actgatgagg ggacaaaaac
    27241 tcacgcagat ctttacgatt ctggcgctga cacaaagtgc aggcattact tttttacagg
    27301 gcgtagagag cgtcagagaa acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa
    27361 tccagcacga tatcagtaac ggtcatccca tctggctggc gctaaaaaat gccggtgagt
    27421 ttagcccgct ctgtttgcaa ttagtgagaa caggagaggc atccggctcg ctggacctca
    27481 tgttagacaa cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg
    27541 cagccttact ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg
    27601 tggcaatgta tctgccaatt ttccatttag gcgatgcgat gagtgggatg ggataacgct
    27661 ggcgtgttgc tccacgccag cgttgggaga ttacaggttg ttgaagatgc ggttttcttg
    27721 ttccagcaca cgaataaacg tggtgcgctt ggtcagctct ttcaggcgtg aagccccaac
    27781 gtatgtacaa gctgaacgca ggccgcccaa aatatcacgc gcggtatttt caaccgggcc
    27841 tcgcagcggc agcttaacgg ttttaccttc tgctgcgcgg tattccgcaa cgccgccaac
    27901 gtgacgtttc atcgcagact cggagctcat gccgtagaac agcataaatt tctcgccgtt
    27961 ctcctcaacg atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc caagcatgac
    28021 gaaatcggca ccgccgccaa aggctttcgc cacatcgccc ggcgtggtac agccaccgtc
    28081 gctgacgatc attccgccca gaccgtgcgc cgcatcggca cattcgatta ccgcagaaag
    28141 ctgcggataa ccgacgcctg ttttgacgcg agttgtacaa acagaacctg ggccaatgcc
    28201 aactttaacg atatcagcac cggagaggat aagctcctca cacatttcac cagtcactac
    28261 gttgccagca caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac
    28321 gaagtgttcg gaataaccat tcgccacatc aatacaaacg aagtttaatg ccgggttcag
    28381 gtcgagaatc tgtttagttt tttcgaaatc cgcatcagac gtgccggtag aaaccatcac
    28441 atgtttcagc acatcagcgg aagaattgtt gataaacgct tgccactctt cgacagaaaa
    28501 gtgtttatgc acagcagtca gaatatcaaa agaagccagc gcagaggcca tagaaaatgt
    28561 gcctacggtg tccatatttg cggcgataat cggcacgccg gaccagctct gacctgaatg
    28621 tttgaaggtg aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt
    28681 agggcggatg agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg
    28741 attcctgggg ttaatggcta taagggtaag agcgcaactc cagtgacgct atcatacgca
    28801 ctaatcaatg cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt
    28861 tacctggtaa atagtgagcg aacgtttcat caatttcact ttcgcgcgct catttttaac
    28921 gttttgataa tcatgatgaa ttcccgggaa tataaattat ggggtatata gttgccttaa
    28981 cgggaggcat tggcagtggt aagagtaccg ttgccaatgc gtttgctgat ctcggaatta
    29041 acgtcattga tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac
    29101 atgccattgc tgatcacttt ggcgaaaaca tgattgctgc tgatggaaca ttgcagcgcc
    29161 gggccttgcg cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgacctgc
    29221 tgcatccgct gattcagcaa gagacgcaac accagatcca gcaagcaact tccccctatg
    29281 tactgtgggt tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc
    29341 tggtggtgga tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa
    29401 ctcgcgaaca tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg
    29461 tggcagatga cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc
    29521 gcctgcacgc acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat
    29581 gcagacccag gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat
    29641 tgagtttttg attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct
    29701 gcatttcttc cgtaatgtca gtgaattact ggatgttttc gagcgcggcg aagtccgcac
    29761 tgagctgttg aaagaactta accggcagca acgtaaactc cagacctgga ttggcgtgcc
    29821 tggcgtggac cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt
    29881 attaatttcc gcgccgcgta tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt
    29941 gcgtcagcga ctgagcatcc caggcggctg ttgcagcttt gatttaccta cattgcacat
    30001 ttggctgcat ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct
    30061 gaacccgctc acccaggcac ttaccatggt gctggattta attcgccagt cggccccctt
    30121 ccgtaaacaa accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct
    30181 gcgcctgaat ctgtcgctcg attcacagct ttatccgcaa atttccggac ataagagccg
    30241 ttttgccatt cgttttatgc cgctggacac tgaaaacgga caggtaccgg aacgtctgga
    30301 tttcgaactg gcctgttgct aaggagtaaa aatgtcagaa actattacgg tgaattgccc
    30361 aacctgcggg aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa
    30421 acgttgtcag ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag
    30481 tggcgatctt tccgaaagcg atgactggag cgaagagcca aagcagtgac atttgtctga
    30541 tgcctcacgt aggcctgata agacgcggac agcgtcgcat caggcatctt gtgcaaatgt
    30601 cggatgcggc gtgaacgcct tatccgacct acagacgctt aagcttcgca attaccggtt
    30661 cattggctgg cggaaaatca tcggcattaa gaccgaccag cgacatccac tcaccgggtt
    30721 gcccttcttt accccacggc accccttccc aactttcgac cagccagaac cacagagtta
    30781 tatgcctgtc cgggaattca tattccagtt tttcaaatag cgaaaaatgt tgtggggtaa
    30841 tcccgacctc ttcctgaagt tcacgcacca ccgcctgttc cggcgtttcg cccatttcaa
    30901 ttttaccgcc gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgtgtta
    30961 taaagatttc attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg
    31021 tcttaaacct ataaaaaagg cgcagaatcc tgcgcctttt acttcaactg ttagctttta
    31081 ttgcaggcga ccatggcact gcttgtattt tttaccagaa ccgcacgggc aaggatcgtt
    31141 acgtcctact ttgcgtttac cggtttgcgc cgccagtgca gctgcggctg cagagtcgtc
    31201 atcctgatgg ctaagctgct gcatttgtgc taaacgctcg gcttccatac gacgctgttg
    31261 ttccagctcc tcaacctctt caggcatacg tacctgaact ttgctcagcg tactgataac
    31321 ttcatatttc aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc
    31381 ctgcttcgga tctttctgtg catagccacg caggtggata ccctgacgca gatagtccat
    31441 cgctgccagg tgctctttcc acagggagtc aagcgtttgc agcatgacgc ctttctcgaa
    31501 gtgacgcatc atctcagcac caaccacttc ttctttacgc tgatacactt cgatggactg
    31561 cgccagaatg cgctcacgca gcgtctcttc atgcagttct ggttctttat ccagccactc
    31621 ggcaattggt aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccggaatatc
    31681 ccacatttct tccagcgact gtggcggaat gtaggcatca atggtggctt tgaacacatc
    31741 ttcacgaatg ctgttgatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg
    31801 ggagtaaatg gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg
    31861 aatgtcgaag ttacgacttt caactttacg ctgggcgttg gcaatcgctt tagtcaccca
    31921 cgggtgttca atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg
    31981 gtcggaagca aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc
    32041 agcatccccc tgacgaccag aacgaccgcg caactggtta tcgatacgac gggattcgtg
    32101 acgctcggta ccgatgatat gcaggccacc tgcttccagt accgcatcgt gacgtacctg
    32161 ccagtcggct ttaatttttt caatttgctc tgcggtcgga ttttccagcg cggcaacttc
    32221 tgcctgccag ctacctccga gcacaatatc tgtaccacga cccgccatgt tggtcgcgat
    32281 agtcaccgca gccggataac ctgcctgagc aacaatcgcc gcttcgttgg cgtggaattt
    32341 ggcgttcagg acattgtgct taataccggc tttggtcagt tcgtttgaca ccagctccga
    32401 tttttcgatg gagatagtac ccaccagcac cggctggcct ttcgcagtac gttctttgat
    32461 atcttcaatg atcgcctgaa ttttttccgc ttcagtcatg tagactaggt ccggcagatc
    32521 tttacgaatc attggacggt tggtcggaac aacgacggta tccagtttgt agatggagct
    32581 aaattcgaaa gcttcggtat cagcagtacc ggtcatcccc gccagttttt catacagacg
    32641 gaagtagttc tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc
    32701 ttctttcgct tccacagcct ggtgcagacc atcggaccag cgtcggccct gcatggtacg
    32761 accggtgtgt tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcacg
    32821 ggtaaacagc gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc
    32881 cggagagtac agagactccc cttcatccat gatgccctct ttcaccagca gttcttcaat
    32941 cagcaccaga ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca ccgagaagtg
    33001 gccttcgccc tggaaggttt cggagtcttc tttttcctga cggatcaggt gcggaataat
    33061 tttattcacg cgtttataca tttccgagct gtcttctgcc gggccggaaa tgatcagcgg
    33121 tgtacgcgct tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt
    33181 acgctgtaca cgttcttcag ggctgaacgc catgttgtcg cgcaggtagt caaagccgta
    33241 ttcgttgttc gtaccgtaag tgatgtcagc tgcgtaagct tcgcgctttg ccggtgctgg
    33301 catgcccggc aggttgatac caacagtcag gccaaggaat tcaaacagcg gacggttgtt
    33361 ttcggcgtca cgttgcgcca ggtagtcgtt gacggtaact acgtgcacgc ctttaccggt
    33421 tagtgcgttc aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat
    33481 ttcggcgatg cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg
    33541 cataccaaag acgcgcttac ttgcctcacg caccacggcg aaagcttccg ggatcagatt
    33601 ttccagcact tcgccttttt ccagacgcgc acgaaactct gcggttttcc ctttcagttc
    33661 ttcatcggag agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg
    33721 catccggcgc agggtgcgat cgttacgact accgaaaact ttagttaaca atttgattag
    33781 cataataaaa tctcaaacgc cccgcgttgc ggagttaata aaatgaagta aaggtttatt
    33841 gttgttaggt gaggcgttga gggccagcac ggatgccttg cgcctggctt atccagacgg
    33901 gcgtgctgaa ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa cccttttcag
    33961 acggcgtgcc ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca agatgttgcg
    34021 cctgaagagg caaagattct tcagcaacgg gcagtgtttg cggtgccatt gcgaaagaaa
    34081 gatgacggat taccgtgcga atagcatgtt gatgccagta atcaacggaa tagttcgaat
    34141 tcggccggcg cgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag
    34201 gctcgtggtt gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg
    34261 caggcaaacc taaactcgcc gcgaccatcc ctaataagag atgcggccag aagtagcgtt
    34321 taccaaactg tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcgaatt
    34381 caaacgtatt tagcgcacaa atcttatcat taaagcctga agtccacagc acgaatgaag
    34441 ataagttgtg cgtaaaagtg cttaagaaaa cagcgttcgc accgttttta tagctcttat
    34501 aaaattcaaa aaaaaacgac tggttcacct agccggagag agtgccagat ttgccagtcg
    34561 aattttatac gacagtataa atgtcgttat gccagtacag ctgaaggcgc tttgaaggcc
    34621 aacggcagtt ctgcgtcgtc ctggaaggtc acatattccc aggcttcctg tttcgccagg
    34681 acagcctgca gcagtttgtt attcagtgca tgaccggatt tataagcggt aaatgcacca
    34741 ataatattgt gaccacacat gaacaagtca ccgatcgcat cgagcatttt gtgacgcaca
    34801 aattcgtctt caaaacgcag gccgtcttcg ttcagtacgc gataatcgtc aacaacgatg
    34861 gcacaatcga agctgccgcc caggcacaaa ccacgggact gcagatattc gatatcacgc
    34921 atgaaaccga acgtacgcgc acggctgatc tggcgcataa acgcatcagc ggagaagttc
    34981 atcgcatagc gctggttgct ggaatcaata gccggatggt taaaatcgat ggtgaaatcc
    35041 agcgaaaaac cattgtacgg cttaaattca gcccacttat cgccatcttc gacacgaaca
    35101 gtctctttga tgcgaacaaa ttttttggcg cagttcaact cgtcgatacc ggcgtcaagc
    35161 agcaggtata caaacggagc ggcgctgccg tccatgatcg ggatttccgg cgcgttaact
    35221 tcgataacaa tgttatcgat gcccaggccc gcgagagcag cattgaggtg ctctacggtt
    35281 gaaatccgta catcatgctc gttgaccaga cacgtacaga gcatggtatc acgcacagat
    35341 ttggcatcgg ccgggaaatc taccggtgga ttcaagtcgg tgcgacgata gatgaccccg
    35401 gtgttggccg gcgcagggcg taacgtcagg gtgactttct tgccggtatg taaaccgaca
    35461 cccgtcgcct gaacgatacg tttaagtgtc ctttgtttga tcatcgtatt atctcgccaa
    35521 attacctatc caaccgaagt gtactataca ttcggcgggc cagtttagca caaagagcct
    35581 cgaaacccaa attccagtca attcttaatc agcttgctta cgtaggaatg ctgggatatc
    35641 cagataatcc ggctctttcg cagtttgcgg cgcattgtca ttcactactt tagcaaccgg
    35701 cttctgctcc tgggtcagcg gagccatccc atgctgctgg tagcgatcca tcactggctg
    35761 ctgaacctgc ttattggtca ccagagtgat ttcaggacgt ttgtccatgc cgatacctgt
    35821 cgcaacaacg gttacgcgca gctcgtcatt catatccggg tcaagagaag taccgataac
    35881 cacagtcgcg ttgtcggaag caaatgcacg gatggtgtta cctaccgttt cgaactcatc
    35941 cagacgcagg tcgaagcccg ccgtgatgtt aaccagcacg ccgcgcgcgc cagacaggtc
    36001 gatatcttcc agcagcggag aagagatagc catttcagca gcttcttccg cacggtcttc
    36061 accgctcgcc acgccagaac ccatcattgc gtagcccatt tcagacatta cggtgcgtac
    36121 gtctgcaaag tccacgttca tcaaacccgg acgagtaatc agttcggcga taccttgcac
    36181 agcgcctttc agtacatcgt tcgctgcgcc aaacgcatcc agcagggaga taccgcggcc
    36241 cagaactttc agcagtttgt cgttcgggat agtgatcaga gagtccacat gcttggacag
    36301 ttcagtgatc ccctgctcgg cgaatgccat acgcttcttg ccttcaaagt tgaaaggctt
    36361 agtgacgaca gcaacggtca ggatacccaa atcttttgcc acttcagcga cgactggtgc
    36421 tgcacctgta ccggtaccac cacccatacc cgcagcaata aagaccatgt ctgcaccttc
    36481 cagcgccgca cgcaatgcat cgcgatcctc atcagccgca ttgcggccaa cttctggatt
    36541 agcgccagcg cccagtcctt tggtgatacc gctaccgatt tgaatcgtct gtccaaccgc
    36601 tgttttacgc agcgcttgtg catcggtatt taccgcgaag aattcaacac cttcaatacg
    36661 ctcgcgcacc atgtgttcaa cagcattacc gccgccgccg ccgacgccga tgactttaat
    36721 caccgcgtca ttggtaagtt ccattggttc aaacatagtt tctctccgat ttgtgcctgt
    36781 cgcctgaggc cgtaatcatc gtcggcctca taaaaattaa aactcttttc gcagccaact
    36841 attgagtcgc ttgatccacg agccaactga tgctgtaaca cgtttttcta cttcagcttc
    36901 accgttaaga tgtgactctt tcccatagtg aagcaatccc accgccgtcg aataatacgg
    36961 ctcctgagca taatccgtta aaccggtaat gttcagcggc gcgccgatac gcacttgcgt
    37021 atgaaacacg cgctgagcac aggctgcaag accttcgatc tgcgctgcgc caccggttaa
    37081 tacaatgcct gccgccaggt gatgtttaac cccttgttgg cgaagctttt cctgcaactg
    37141 caatatctct tcgttgacca ggttgagcaa ctcggtatag cgcggctcga tcacctctgc
    37201 cagggtctga cgttgcagac tccgcggcgg acgaccgcct acgctcggca cttccacgct
    37261 ctcatctttt ccaacgatgg aacccagcgc acaaccgtgg cgaactttaa tcgcttcggc
    37321 gtcgcttggc ggcgtgccaa aggcgtaagc gatatcactg gtcacgacat tgcctgcata
    37381 aggaattacc ttagtgtggc gcaatgcccc accggtataa acggcgatat ccattgtacc
    37441 accaccgata tcgacgacgc agacacccag ttcacgttca tcttccgtca ataccgaata
    37501 acttgatgcc agtccggcaa atatcagttg gtcaactttc agcccacaac gttcaaccgc
    37561 tttgacgatg tttttcgcca tatcgttgtg acatgtgatc aggtgcactt ttgcctgcat
    37621 ccgcacaccc gaaagtccta ccggattctt gatcccttcc tgatagtcaa tcgcatactc
    37681 ttgcgggatc acatgcagca cacgatgctc atcgcgcaca cgcaccgatt tcgcggtatg
    37741 gacgacattt tccacatctt cttgcgtcac ttcttcttca gaaataggca ccataccaat
    37801 ttcattctgg cagctgatgt gcttaccaga aagcgccaga tataccgaag agatctgaca
    37861 atctgccatc aattctgcct ggtcaatggc gcgttgtacg cacttgacca cggattcgag
    37921 gtcgttcacc ccgcctttat ccataccacg tgacgggcag ctgcccacgc caatgatatt
    37981 gaccataccg tcgggcagaa cttcccctac taaagcggca accttcgcgg taccaatttc
    38041 cagtcctact accagttttc tgtccgtcgc cttgatcatt gttgttctgc ctgtgcctga
    38101 ttttgttgct gagtagattc ctctggcggc aagggcgccc agcctactgc cgctccagag
    38161 tcataacgca aatcaacgta gctaatccgt ttgccatcgg tttgcgcctg ctgctgtaaa
    38221 accggataaa gttctacaaa gcgagccaaa cgtttcatcg tatcgccccg gccaagattg
    38281 agcttaatat cgttattcag cgtcaactgc caggaacgcc gcgcggtcat cgccgcttcc
    38341 ttcagagtaa atctgtcctt tgccagcatc tgccccattt cgcgatagcc ctgcaacact
    38401 tcattagcgc tgccttccgg gccatacagc attggaagca cctgcttgct ggtgcgatct
    38461 ggcggcacgc tgaaggtgtt tccttccgcg tctaccatgt gttgatcatt ccaccgcgca
    38521 atcggcacat attcaaccag atgaatcttc aattcatcag gccactgctt tctgacgctc
    38581 acctgcttaa tccacggcag gcgttgttct atttgcgtct ggatgatgtt gacatcctgg
    38641 gtcataaagg tacccggctc acccaatgcc aggatcgact gccggatatc gtcattacgc
    38701 gtgtaatggc gttcaccggt caacaccagc tttgagagcg gcaggcgttg cgcatcttcc
    38761 atccagctca acacgaccca gccgctcacc aacactgtcg ttaaaacggt cagcaggaaa
    38821 aggatccccg ccagacgcgt tccattattg cggcgagaag aaacctcttc ttcgctgttt
    38881 cgcgtgttca gagcagcctg cgacatatta gtccgccagt tccagaattc gtactaccaa
    38941 ctgcgagaag ctcatacctg cctgacgtgc cgccatcggc accaggctgt ggctggtcat
    39001 acccggtgag gtattggctt ccagcagata aaactgtcca tcgctgtcca gcataacgtc
    39061 aatacgtccc catcctttgc aacctaacgt cgtccatgct ttcagcacta atgcctgcaa
    39121 attggcctct tgtgacgctt ccagacctgc ggggcagaaa tactgtgtct catcagagag
    39181 atacttcgcc tcataatcat agaaggttcc ggacggttga atacgtattg acggtaaaat
    39241 ttcttcaccg agtatcgcaa ccgtgaactc cggcccactt agccattttt caatcaatac
    39301 ttcttcatcg tgctgaaatg ccaatcttaa tgcatcttgt agagcatttt ctgctactac
    39361 ttttgacatt cccacactgg aaccttcgcg gctcggctta acgataaccg gcaaacccag
    39421 agcagaaatt tctgctaact gcttatcgct caggcctttt tcaaactctg cgcgggttaa
    39481 tgctacccac ggcgcgaccg gtaaaccggc accttgccac agaagtttgc tgcgtagttt
    39541 atctattgaa agcgcagatg ccatcactcc gctgccggta taaggcaagc ccatcagctc
    39601 gagcatgccc tgcagcgtac catcttcacc gccgcgaccg tgtagcgcga taaacacttt
    39661 ctgaaaaccc atcgacttca gttgcgtcac gtcgacttct ttcgggtcga caggatacgc
    39721 atcaataccg ccttcacgca gcccggctaa caccgctgcg ccagaattca gagaaacttc
    39781 ccgctcagcg gaagtcccgc ccaacaggac cgcgatttta tcagtcatgt tgttcttcct
    39841 ccggagtttg cggcttcagt ttgatttcag ctaaagaacg ggcaattttt ccgatattac
    39901 cagccccctg aacgagaatc aggtcgttac cggttaatac cggtgccagc atctcggcta
    39961 cctgcgcagg gtccggcacc agaatgggat caattttccc acgtccacga attgtgcgac
    40021 acagcgaacg gctgtccgct cccggaattg gcgcttcgcc agccggatac acttccagca
    40081 tcaacagggt atcaacctgc gtcagcacat tggcgaaatc atcatacagg tcgcgcgtac
    40141 gggtaaaacg gtgcggctga aacagcatta ccaggttttt atccggccag cctgcgcgcg
    40201 ccgctttaat ggtggcgtca acttccgtcg ggtggtggcc gtagtcatcg accagcattg
    40261 ccgtaccgct tttaccattc actggctcca gcgggaattc accgaggaaa tcaaaacggc
    40321 gaccagtccc ctggaagctc tccagcgcac gcaaaatagc ctcgtcgtca attccctctt
    40381 ccgtagcaac cgcaaccgca gctgcggcgt tcagcgcgtt atgacggcct ggcgcattca
    40441 gggtgacgcg catcggctct ttgtcctgac gcagcagcgt aaagtgcccc tgcgggccaa
    40501 tctgctgata atcttctaca cgcacgtcgg catcttcgct gaagccgtaa gttgtggtct
    40561 gacgtcccac acgcggtaac aattcgcgga tcaccggatc atcaacacac atcaccgcac
    40621 gaccgtaaaa aggcaggttg tgcagaaaat taataaaggt ctgttttaaa ttctcaaagt
    40681 cgccctggta ggtatccatg tggtcggctt cgatattggt gacaatcgcc accatcggtt
    40741 gcagatgcag gaacgatgca tcactctcat ctgcttcggc aatcaggtac cgaccatgcc
    40801 ccaaacgcgc atgaaccccc gccgctttta ccagcccgcc gttaacgaag gttgggtcga
    40861 gccccgcttc tgcgtagatg ctggaaacca tcgcggtagt tgtcgttttg ccgtgcgttc
    40921 ctgcaatggc gatgccatga cgaaaacgca ttaactcagc cagcatttcg gcacgacgga
    40981 tcaccggaat acgcgcttca tgagctgcga caatttccgg gttgtcggca gaaatggcgc
    41041 tggaaacaac gaccacgctg gcatcacgta cgttttccgg gcgatggttg aaataaatcg
    41101 tcgcacccag attcagtaac tgctgcgtga ccggatttgg cgctaaatcg gaaccactga
    41161 tctgataacc ttcattggcc agaacttcgg caataccgcc cataccggca ccaccaatgc
    41221 cgacaaaatg tatgtgccga acgcgacgca tttcgggcac gatggaacgc agttttgcca
    41281 attgttgtgt attcattctt tacgccatta acttcttaaa ttcatacgat gcaaaaggca
    41341 tcgctacaat tacgcccggg cagcccggct cacttcattt gccactcgct cggtggcatc
    41401 cggaatggat gcagcgcggg cgcgttctgc catggttaat aaggtttctc gcgaccaccc
    41461 ggccagggtg ttggcgacag catccacgct aagctgtggc tgctcgataa ttttggctgc
    41521 gcccgctttt tccagcggta gcgcattcca gtattgctgg cggtctttat gttgaaacgg
    41581 cacaaacaac gccggtagtc ctgccgcggc gatttcactc acagttaaag caccggagcg
    41641 gcaaacgacg acatccgccc acgcatacgc cgccgccata tcatcaataa attcagtcac
    41701 tttatgctgc ggttgccccg cttcggcata cgcctgttca acggattgtt gcgaaccttt
    41761 gccgctctga tgccagatag tgactgaatc acccagtttc gcagcaacct gcggcattgt
    41821 ctggttaaga atgcgtgcgc cctgggaacc acccactacc aacacacgaa ccggaccttc
    41881 acgtccagcc aaacgttgct gcggcaacgg cagcgccagc acatcggtac gcaccgggtt
    41941 acccaccact tccgcattag ggaaagcgcc tggaaacgcc tgcatcactt tggtagcaat
    42001 cttcgccagc catttattgg ttaagcccgc aataccgttt tgttcatgaa gtacaaccgg
    42061 aatgcctaac gaccacgcgg ccagaccacc tggacctgat acatagccgc ccataccgag
    42121 taccacgtca ggtttgtacg ctttcataat cgcccgcgcc tgacgccagg cgttgaagat
    42181 acgcagcggc gcagctatca gtgcttttat accttttcca cgcaaaccag agatacgaat
    42241 gaaatcaatt tcgatgccat gttttggcac taagtccgct tccatacggt cggcagtccc
    42301 cagccagcga acttgccaac cctgagccat tagatagtgc gcaaccgcca gtcccgggaa
    42361 tacatgtcca ccggttccgc ctgccatcac cattaatcgc tttccttgac cactcatcgt
    42421 gaacctcgta caaacgcctg cgctttctcc agacgcgttt cataatcaat acgcaacagc
    42481 atcatgatgg ctgtcgacat aatcagtaag ctcgaaccac cgtaactgat tagcggcaat
    42541 gtcagacctt tggtcggtaa catccccgcc gccgcgccta cgttaaccag cgcctggaag
    42601 ctaaaccaga tgccaataga acaggcgaga aaaccggaaa aacggtggtc aatttctaat
    42661 gctttgcggc caatcgacat cgcacgaaaa gcgacgaaga ataccattaa aagtgctagt
    42721 accacaccga cataacctaa ttcttcgccg ataatggcga aaataaagtc agtgtgcgct
    42781 tccggcagat actccagttt ttgtaccgaa ttacctaaac cttgccccca aagttcgccg
    42841 cgaccaaacg ccatcagcga ttgcgttaac tgatagccgc tgccaaaggg atcttcccac
    42901 gggttccaga atgcggtaac acggcggata cggtacggtt cggcgaggat cagcaacaca
    42961 accgctgaaa tgcccatacc gataatggca atgaactgcc acaatttcgc tcccgccagg
    43021 aacaacatcg ccagcgtagt cacaaacaac accaccaccg taccaaggtc tggctgtgcc
    43081 agcagtaaca ctgccaacac cagaatcacg cccatcggtt tcaggaagcc gcgcaggtta
    43141 ttacgtactt cgtcgccttt acgcaccaga tagttggcga tatagcaaaa cagcgacagt
    43201 tttgtcagct ccgcaggctg gatacgcagc aaaccgagat cgatccaacg cgatgcccct
    43261 ttaacagcgc tacccactac cagcacgatc atcagcagga tgatagatcc gagcagcatc
    43321 gtggcactgt agcgttgcca gaactccatc ggcagacgca gcgtaatgat cgccagaata
    43381 aacgccaaaa tcagatagac accatcacgc ttcgcgaaga agaacggatc gttggttaag
    43441 cgttgcccta tgggcattga cgccgaggtc accatgataa agccaatcgc cgcgaggccg
    43501 aaggtcagcc acagtaaggt gcgatcgtac atgatcaggc tgtcggtatc tttttcccgc
    43561 gagcccatca cccagccctt tagcgccgtg gagatccaga ccaggatact gaatcctggc
    43621 aggcgcggca ttttcaggcg agggagagat aaacgcatca acctaactcc ttcgccagac
    43681 gggcaaactc attgcctcgt tgttcaaagt tcttgaactg atcaaggctg gcacaggccg
    43741 gggagagcag aaccatatcg cccggctgaa cacgcggagc cagcaagcgc atagcctgtt
    43801 ccatagtttc ggtttgttct gccacttccg ggcgtagcgc cgccagctgc gcgccgtcac
    43861 gaccgaaaca atacagacgt acgttatcgc cattcaggta acgcgccagt gggctaaagt
    43921 ccgccgattt accatcgccg cccagcaaca aatgcagcgt gccgtctacg tgcaggccat
    43981 tcagcgccgc ttccgtactg ccgacgttgg tcgcttttga atcgttaatc cagcgcacac
    44041 cgttatgctc cagcaccact tcaaagcgat gcggcagacc ggtgaaggtg gttaacgctt
    44101 tcaggctgct ggcgcgcggt aaccctgcgg catctgccag cgccagcgcc gccagcgcat
    44161 tggtgtagtt atgctgcccg gaaagtttca tctctttcac attcagcact ttctcgcctt
    44221 taacccgcag ccaggtttcg ccctgttgat gattcaggtg atagtcaccc atgttgacgc
    44281 caaagctgac gcagcgttca tccgcaccgc gaatcggcat tgttaaggca tcatcagcat
    44341 taaccacgca aactttcgcg ttttcgtaaa tgcgcagttt tgctgcacga tactgttgta
    44401 aaccaaacgg gtagcgatcc atatgatctt cagtcacgtt cagaatggtc gctgctaccg
    44461 cctgtaagct ggaggtggtt tccagctgga agctcgacag ttccagcacg tacagttcac
    44521 actcatcatc cagtagcatc aacgcaggca ggccaatatt gccacccaca ccaacgttaa
    44581 cccccgccgc tttcgccatt tcacccacta gcgtggtgac cgtgcttttg ccattagaac
    44641 cggtaatcgc cacaatcggt gcttgtgctt cgcgacaaaa cagctcgata tcgccaacga
    44701 tttcgattcc ggcatcagcg gcagcgctta aggatggatg cgccagtgca ataccgggac
    44761 tggcgacaat cagatctgcc gccatcagcc attcatcatt cagactgccc gtgtggcgtt
    44821 ctacggcttc gggtaattta tccaggccag gcggtgtcat acgcgtatcc ataacgcgcg
    44881 gcgtcacacc gcgagcgagg aaaaagtcca cgcaggaaag cccggtgagg cccaggccga
    44941 taatgacaac atttttaccc tggtaatcag ccatgattaa cgtaccttca gcgttgccag
    45001 accaatcagg accagcatca gcgaaataat ccagaaacgc acaatgacgc gcggttccgg
    45061 ccagcctttc agttcatagt ggtgatgaat cggtgccatg cggaaaatac gttgtccgcg
    45121 cagtttaaag gagccgacct gcaggatgac agaaagcgtt tctaccacga acacgccccc
    45181 cataatcacc agcaggaatt cctgacgtag cagtacggcg ataatgccta acgcaccacc
    45241 taacgccagc gaaccgacat cgcccataaa gacctgcgcc ggataggtgt taaaccacag
    45301 gaagcccagt cctgccccga ctatcgcggt acagacaata accagttccc cggcgtgtcg
    45361 cagatacggt atatgtaaat agctggcaaa gttcatgttg ccggtcgccc acgccaccag
    45421 cgcaaaacca ccggcgacaa atacggtcgg cataattgcc agaccgtcaa gaccatcggt
    45481 caggtttacc gcgttgccag tacccacaat gacgaagtaa gccagcagaa tgtagaacag
    45541 ccccagttgc ggcatcacat ctttaaagaa tgggaccacc agctgcgttg cgggcgtgtc
    45601 tttgccggca aggtacaaag cgaaagcaac acccagcgca atgaccgaca tccagaaata
    45661 tttccaacga gcgatcaacc ctttggtgtc tttacgcacc actttgcgat aatcatcaac
    45721 aaagccaata acaccgtaac ctaccagcac caccaacacg caccagacgt acggattgga
    45781 cgggtaagcc cacagcagca cggagatcac aatcgctgtc aggatcataa tcccgcccat
    45841 ggtcggcgta ccgcgcttgc tgaagtgtga ttcaggaccg tcgttacgca ctacctgacc
    45901 aaaggaaagt ttttgcaaat gagcaatcat gcgcgggccc atccacaatg agatgaacag
    45961 cgcggtcagc aggctgacga tggcgcgaaa cgtcagatag gaaaagacgt taaagccgga
    46021 ataatatttg accaaatgtt cggccagcca aactaacatg tcccattctc ctgtaaagcg
    46081 cgtactacct cttccatggc agcactacgt gaacccttaa ctaaaatcgt aattacctgt
    46141 tgctcagcaa tcagtgattt aagacgcgta attaacgcag ttttatcagc aaaatgttcg
    46201 ccaacgccgc tggcggtgct gatagcatgg ctttgtttac ccatgcttaa cacgcggtca
    46261 ataccagcag cttttgccgc ctcgcccacc tgtacatggc aggcttcgct ttcagcgccc
    46321 agttccgcca tatcgcccac caccagcacg cggtagcccg gcatttcagc cagtacctga
    46381 acagctgcgg tcattgaacc gacattggcg ttgtaggagt cgtcgagcag caactggttt
    46441 tctgccagtt ggatggggaa cagacggcca ggaacagctt tcagatttgc cagccccgct
    46501 ttgatagcat caagcgttgc gcccacggac atggagagcg cagcggctgc cagcgcattc
    46561 gcaatattgt gacgccccgg caacggcagc agaacatcga cgctaccggt tggggtttgt
    46621 agggtaaatt ccataccgtg cgaggtcaca tggatattgg tggcggtgaa atcgctgttg
    46681 gcggcattgg gtgagaaacg ccacactttg cgtgagccaa ttacgctctg ccagttcagc
    46741 cagtcgttgt tgtcggcgtt cataatggcg ataccgtttt ccggcaggcc gctaaagatt
    46801 tcacctttcg ctttcgcgac acccgcaagc gagccaaaac cttccagatg cgccgctgcc
    46861 aggttgttga ccagcgccgc ttccgggcga gtcagactca cagtccaggc aatctcgccc
    46921 tgatggttcg cgccaagttc aataactgcg taatcgtatt ccggcgttaa gcgcaacagc
    46981 gtcatcggta caccgatgtc gttgttgaga ttgcctgccg tataaagcgt gttgccgcac
    47041 tggcttaaaa tcgccgccgt catctcttta acggaggttt tgccggagga ccccgtcaga
    47101 gcaaccacgc gcgccggaac ttgctggcga acccatgcag ccagttcacc aaacgccaga
    47161 cgcgtatcct tgacgattaa ctgcggcagg tcgatgtcca gcggacggct aaccagtagt
    47221 gcgcctgcgc cgccagcttt cgcctggtcg gcaaaatcgt gggcatcaaa acgttcacct
    47281 ttcagggcaa caaacaggca gcccggcgtc agttttcggg tatcagtggt tacagcatca
    47341 agggtgatat ctgcaccttg cagttcaccg ttgagaatat cggtaagttg gctaagggtt
    47401 acgctaatca tgcaatcacc cccagcagac gcgccaccgt gacgcgatcg gagtagtcca
    47461 gacgctgatt gccaacaatc tggtaatcct catggccttt gcccgcgacc agtaccacat
    47521 cattctcttt agcctgcata acggcgcaag tcaccgcttc agcacggcct tccatcactt
    47581 tggcatgtcc ggcatctaac attcccgcca gaatatcgtt gatgatggca cgcggttctt
    47641 cggtacgcgg gttatcgtcc gtcaccaccg ccacgtcagc aaactcttcg gcaattgcgc
    47701 ccatcagtgg acgcttacct ttatcgcgat cgccaccaca gccaaagacg caccacagct
    47761 tgcccgcaca gtgcaggcgc gccgcctgta aggctttttc cagtgcatcc ggcgtatgcg
    47821 cgtaatccac caccaccgtc ggtttacctg gcgcagtgaa cacttccata cgtccgcaaa
    47881 ccggttgcag acgcgcggcg gttttcagca gatcagccag tggatagccc agcgccagca
    47941 gtgttgccag cgcgagcaac aggttgctga cgttaaaagc gcccatcaga tggctttcaa
    48001 tttcgccatc gccccagctt gaactaaagc gaatggtcgc gccgctgtcg tgatagttca
    48061 cttcggtcgc tttcaaccag cgtccgtgac agttcggatt gatatgatct tccattgata
    48121 ccgcaaccgc gtccggcagt tttgccagcc agcggcggcc cacttcatcg tcggcgttaa
    48181 taatcgcctg accgcaatga tgttcagaat aaagcagcca tttcgcagct tcgtagtgtt
    48241 ccatatcacc atgataatca aggtgatcgc ggcttaagtt ggtaaagacc gacgccgcaa
    48301 atttcaatgc cgccacacgg tgctgtacca gcccgtggga ggaaacttcc attgcgcaaa
    48361 aagtcgcgcc ctgatccacc agccccgcca gctcatgctg aacatcgact gccgaaccgg
    48421 tagtattttc tgtcgggatc actttcccca gcagaccgtt accaacggtg cccattaccg
    48481 cgctggtttc gccaagcagt tggctccact gcgccagaag ctgggtcgtc gtggttttgc
    48541 cgttggtgcc cgttacgcct acgagacgta aattgtcaga gggttcatgg taaaagcggc
    48601 ccgccagtgc agataaacgc tcgttgagct ggctgagata gatgaccggt acgccgtgca
    48661 tttcacggat ttcaccgtcg gtcgcctcat ctttcgcctc tgcaataatg gcagccacac
    48721 cttgcgctat cgcctgcggg atatatcgac gcccgtccgc ctgatgacct actacagcta
    48781 caaagagatc gcccgccgca gccacacggc tgtcgagtgt catctctcgc agtgctcgcg
    48841 aaggtgcgtc tggcacccac ggagcaagaa ggtcgcgcaa attacgatct gccacctgtc
    48901 ccctcgcctt gattaatcac aaattcattt ttatcgcccg ttgtcagcgc atccggctcg
    48961 atgttcatgg tacgcaatac gccgcccatg atggtaccaa agaccggcgc ggaaacggcg
    49021 ccgccgtagt atttacccgc ctgcggatcg ttgataacaa caaccagcgc gaagcgcggc
    49081 tgactcgcag gcgcaacgcc tgcggtataa gcaatatatt tattgatgta gcgaccgtcc
    49141 ggcccgacct ttttcgcggt accggtttta atggcgatac gatagccttt aatcgccgcc
    49201 ttcacgccgc cgccgcctgg tagcgccacg ctttccatca tatgcaccac ggtgcgaaca
    49261 atggattccg ggaagacacg ttcaccggga accggggggt caactttggt aatcgacagt
    49321 gggcgataaa tgccgtagct gccgatagtt gcgtagactc gcgctaactg taacggtgtt
    49381 accattagcc cgtagccgaa agagaaggtg gccctctcta tgtcagacca ccgttgtttt
    49441 tgaggatata agccactgcg ttctccgacc aaccccaaat tggtcgcttt tcccagtcca
    49501 aaacgtgagt aagtatctac taacgctgag gacggcatcg ctaacgccag cttggaaaca
    49561 ccgacgttac tcgacttctg taataccccg gtcagggtta attcgctgta gcgtgccacg
    49621 tctttgattt cgtggccgtt aattcgataa ggaatggtat tgagtaccga gttttcccgc
    49681 accacgccac gttgcaacgc ggtcatcacc accatcggtt taaccgttga gcccggttca
    49741 aacacgtcgg tgatggtacg gttacgcatc gcctctttcg gcgtgccgct cagattgtta
    49801 gggttgtatg acgggctgtt agccatcgcc agcacttcac ctgtgttgac atccaccagc
    49861 acggcgctac cagattcagc cttgttaaag gccaccgcgt tgttcagttc gcgataaacc
    49921 agcgcctgca ggcgttcatc aatactcagc gccaggttgt gcgctgcctg gctgtcagta
    49981 gaagaaatat cttcaattac gcgaccatag cggtctttac gcacaatgcg ctcacccggc
    50041 tgcccggtaa gccatttatc gaaactcttc tcaacgccct caatcccttg actatcgacg
    50101 ttagtaaagc cgatgaggtg agcagtcact tcgccggacg gatagtaacg gcgagactct
    50161 tcacgcagat gaatccccgg cagtttcagt tttttgatgt agtccgccat gtcagggttt
    50221 acctgacgcg ccagataaat aaagcgccct ttcgggttgg cgttaatgcg ggcagaaagc
    50281 tgatccagcg gaatattgag cgcgttagcc agcgccttcc agcggtcacc gacgctgata
    50341 ccgccagcgt catgcacttc tttcgggtca gcccaaatcg cttttaccgg cacgctcacc
    50401 gctaacgggc gaccagaacg gtcagtaatc atgccgcggg aggtggaaac ttgctgaacg
    50461 cgaagagaac gcatgtcgcc ctctttcacc agcatatccg gggagataac ttgtaaccac
    50521 gctacgcgtc cgagcagaaa agccagcgcc aggagaatac agccgcataa caacgcaaaa
    50581 cgccaactga taaagttggc atgttcttcc tgacgttttg gtttctgcgt tttcaccgct
    50641 gctttcatgc gtcgcgttta tccttatttt tgcactacga tattttcttg tgacggatca
    50701 acatgctgca tttgcagctt ttccgtggcg atcctttcca cccggctatg gtcgccgagc
    50761 gcattctctt caaggatcag gttgcgccat tcaatgtcta aagcatctcg ctccagcacc
    50821 agttgttcgc gctgagcggt cagtaaacgg gtatggtgcg ccgtggttac cacggtcacc
    50881 gccgtcaaaa taatgcaaat gaacaggcag agtggcagct tcccaaatcg caaaagatcg
    50941 tcaccgataa caccaggcaa tgcatggcgc tcgtggcttc ccatcgatcc tttaactttg
    51001 cttagagctt ctgtcactct gctgatcatg cgttcgtcct ctctgcaata cgcagaactg
    51061 aactacgggc acgagggttc tcagccacct cttcttcgcc cggcattaac ttgcctagtg
    51121 ctcgcagctg acggccaccc agttttttga gctgctcttc agtcatcggt aaccctgccg
    51181 gaacttgcgg accgcggctg ttttcacgca taaaacgttt cacaatacgg tcttccagcg
    51241 agtggaagct gatgatcgaa agccgcccac ccggggccag cacgttgagc gagcttttta
    51301 gcgcctgctc tatctcctcc agttcactgt ttacccaaat gcgcaccgcc tggaaggtac
    51361 gggtcgcggg atgtttaaac ttatctttca ccggcgttgc agcagccacg acttccgcca
    51421 gttctttggt gcgggtcatc ggctgttcgc ggttacgctc gacaatggcg cgggcaatgc
    51481 gtttggcaaa acgctcttca ccataggttt tcaataccca ggcgatatcg gcttcttctg
    51541 cggtttgtag ccattcagcg gctgactgcc cacgggttgg gtccatacgc atgtccagcg
    51601 gaccatcgcg cataaaggaa aagccgcgtt cagcatcatc aagttgcggt gaagagacgc
    51661 caagatcgag gagaatgccg tcgatcttgc caataagatc gcgctcggca acgtattcgc
    51721 ccagcgcgga gaaaggtccg tggatgatgg agaagcgcgg atcatcaata gtcttcgcaa
    51781 cggcgatagc ctgcgggtcg cgatcgatcg ccagcaaacg cccctcttcg ccaagctgcg
    51841 agaggatcag acgtgagtga ccaccgcgac caaaagtccc atcaatgtag atgccatcag
    51901 gacggatatt gaggccatta acggcttcat ccagcagcac cgtagtatgt ttatagtttt
    51961 ccatcatttt atagagacaa gtcctgcagt cgctccgata agtctccggt agccaactgc
    52021 tctgcgtcga tatcttcctt gacctgttga tgccaggttg tttcatccca cagctcaaac
    52081 ttgttgaact gtccaaccag catcacttct tttgtcagcc cggcatgttg ccgcagtact
    52141 ggcgcgatta acaatcgacc tgcgccatcc atctgacatt cgctggcatg acctaacagt
    52201 aggcgctgca cacggcgctc aaccgggttc atgctcgaca gacgcgataa tttttgctcg
    52261 ataatttccc attcaggcag ggggtaaagc agcaggcacg ggtgatgaat gtcaatggtg
    52321 caaaccattt gaccggcagc gttctcaagc agctgttccc gataacgggt aggcactgat
    52381 aagcgccctt tgctgtcgag attgactaac gttgctcccc ggaacatgcc agcctcaccc
    52441 cttattccca ctttgcccca caatttccca ctgaaaggag tttacggagc gtaggaaaag
    52501 cttgtcaagc cagtcacaag gcataccgag gtaaaaaacc cgatgtttac ggctaatatc
    52561 agcgttgttt attttacgtc caacgtgcga agcaaattaa cgttatgaat agctgtaaga
    52621 aaaaatccct catcgtcatc actgttttaa aatgactcac tattttccac agcaaatata
    52681 aagtgtcagt ttgcgacgcg agcggcattt taggacatat cttccccggt taacagtcct
    52741 tgttacgtct gtgtggcgct tgatgccagc gggtttgcgc aaaagcaaag aaaagtgttt
    52801 gttaaatcgg caattcatcg ccgcatgtaa caaaataatg caaaaacgcg ctgtcgatat
    52861 cacttaatta ttgtgaattt cagctggtga gttaattaac gggcgggttt aaaaccattt
    52921 atcaataata gtcaggaata gtcttattta ctttaaggat attgatgtcc agtcccgtac
    52981 tctacgcgcc agagggaaat tcacctggcg cgtatttttg ttcgcggctt agctacggct
    53041 gagcacgccg cggcgataga gattacgttt aatgcgcgtt aaaccaggtt ttggcttacg
    53101 cggttcgtcc aggcttgcca ggacaatctc cagcacacgc tctgcgacat cgcggtgacg
    53161 ttgagccact gccagcaccg gacactgtaa gaagtcgagc agttcgttat cgccaaaggt
    53221 ggcaattgcc aggtcagaag gcagtttgcc gtcgcgacgc agcgtgacat ccatcactcc
    53281 ttgcaacaac gcaaacgacg ttgtgaacag cgcctgcggc atcggatgcg tttccagcca
    53341 tttttcgaat aactgggcag ccgcctcccg ctcatagctg ttggcataca ggaaatgcac
    53401 ttcgcgcgga tcatctttcc aagcagtacg gaaaccttgt tcacgcagga agctgacaga
    53461 aagctccggc agcgcgccaa gataaagcac cgtctcggcg ggaaacttac gtaactcttc
    53521 cgccagcatt tcggcatcat cctgatcggc accaaccacg ctggtgaaat gttcacgatc
    53581 gagggcgcgg tccagcgcga caatcgggaa cgggtcgtta gcccagcgtt gataaaaagg
    53641 atgctcaggt ggcaacgacg tcgaaacaat aatggcatca acctgacgct gtaaaaggtg
    53701 ctcaatgcag cgcatttcgt tgtctggctg atcttctgag caggcaatca gcagttgata
    53761 accccgttgc cgcgcctggc gttcaagata gttagcgatg cgggtatagc tggtgttctc
    53821 cagatcgggg atcacaagac caatagaacg tgtgcgtcca gcacgaagcc cagctgccac
    53881 ggcgttcggg tggtaattgt gctcacgcac cacagccatg actttttcaa cggttttgtc
    53941 gctcacacgg tattgcttcg ctttgccgtt aataacatag cttgcagtgg tccgcgacac
    54001 tcccgccagc cgagcgattt catccagttt cacaattgcc ccttgcgtaa aatgtaaaaa
    54061 ccatagccat tgtactggta tgggttaaat tcattaacat ctaagcgcag aaaattaact
    54121 gcggcaactg cttttattcc aggttcccac tgatttcgca aaaaaagccc aacgtgacat
    54181 gttgggctgc aaattgcgca ctgagatcat tctcaacgca ttattttatc gccgcgcgaa
    54241 agtccgacca caccagagcg agcaacttcc acaattttcg ccacatcgcg aatcgatgct
    54301 aaaaatgcat caagcttatc gctggtgcct gctaattgaa cggtataaag cgagggggtg
    54361 acatcgataa tttgcccacg gaatatttcc gtattacgtt tcacttcgtc acgcccgtaa
    54421 ccgctggcct gaattttcac cagcatgatt tcccgctcaa catgcgcgcc ctgccccaac
    54481 tcactcacgc gcaagacatc gaccagcttg tgtaattgct tttcgatctg ctcaagtact
    54541 ttttcatcgc ccacggtctg gatggtcata cgcgataatg tctgatcgtc ggttggcgca
    54601 acggtcaggc tttcaatgtt gtagcctcgc tgggaaaaaa ggccaatcac gcgggataac
    54661 gcgcctgatt cattttcgag taagactgat aatatccggc gcataatcag gttctctccg
    54721 ttttgcttaa ccacatttca tccattccgc ccccgcgaat ctgcatcggg tagacgtgct
    54781 cactgccatc gacggtaaca tcaacaaaca ccaggcgatt attgcgcacc tgttccagcg
    54841 cctcgctaag tttgctttcc agctcatgcg gatgagagat ctggatcccg acgtgaccat
    54901 aggcttccgc cagacggacg aaatcgggta gtgattgcat ataagattgt gaatgacggc
    54961 cggaatagat catgtcctgc cactgtttca ccatccccag atagcggtta ttgagattca
    55021 ccaccagtac gggcaactcg tattgcaacg cggtagacag ttcctggatg ttcatctgaa
    55081 tactgccgtc gccagtgacg caaaccacgg tttcttctgg caacgccatt ttgacgccca
    55141 gtgccgcagg taaaccaaaa cccatcgtgc cgagaccgcc ggaattgatc cagcgacgcg
    55201 gtttgtcgaa tggataataa agcgcagcaa acatctggtg ctgcccgaca tcggacgtca
    55261 cgtaagcgtc cccattagtc aaccgccaaa gagtctcgat caccgcctgc ggtttaatct
    55321 tttcactgtg agtgtcatat ttcaggcact gaagagcgcg ccactgttca atctgctgcc
    55381 accagtcgcg gatctcatcc agcggttgat gggcggattc ttgcgacagg agttcaagca
    55441 tttgttcgag gacctggcga gcatccccca caatcgggat atccgcagtc acggttttag
    55501 aaatggaagt aggatcaata tcgatatgca gaacagtggc atttgggcag tactttgcca
    55561 gattgttcgt cgttcggtca tcaaatcgta ccccgacggc gaaaatcaca tccgcgttat
    55621 gcatcgtcat attggcttcg taggtaccgt gcattcccag cattcccagt gcctgacgat
    55681 gcgttgccgg aaacgccccc agccccatca atgaggaaac aaagggcaga ttcaacgcct
    55741 ccaccgtttc tttcaactgc tgatggcagc ccgccatgat tgccccaccg cctacgtaga
    55801 caaccggttt ttttgccgcg accagcgttt gcagagcacg cttaatttgc cctttatgtc
    55861 cggtagtagt gggattgtaa gaacgcatac tgaccgactc agggcagaca tagggtaatt
    55921 tgttcgccgg attaagaata tctttcggta aatcaacgac cactggtcct gggcgaccac
    55981 ttgccgccag ccagaaagcc tttttcagca cctgcggaat gtcttccgtt tgcttaacca
    56041 gaaaactgtg tttaaccacc ggtcgcgaaa tccccaccat gtcgcactcc tgaaaggcat
    56101 cgtaacctat caacgaggtc gctacctgcc cggaaaggac aactaatgga atggaatcca
    56161 tataagcggt ggcgatgccg gtaatcgcat tggtcgcccc tggacccgac gttaccagca
    56221 cgacgccgac ttccccggtc gcacgcgcca ggccatcggc catatgcacc gccgcctgct
    56281 catgacgaac taatacatga tcaataccac ccacggtatg caatgcatcg taaatatcaa
    56341 ggactgcgcc tccgggataa ccgaatactt gtttaacgcc ctgatcgata agcgatcgga
    56401 cgaccatctc ggctccagac aacatctcca tggcctgcct cactgtttga cggaaaaaat
    56461 gtgtaaaaca cattaaccgt ccgatcttgc ttaagcaatt ggcagccaga gacataaacg
    56521 cagggtaata agaataaaca ggaggaaagg tgaaaagaaa cgataaaatc ctccattacg
    56581 aaggattaaa ttgataataa atcggctgaa tcccacaact tacagcaatt tctctatttt
    56641 tttaattaat cctaaaatag agagtccaca cattcagaat aaaattctac attcaataaa
    56701 aaatgcagaa taaaccagac attcatgtct ggcctattct gcattcagtt agcgtttgca
    56761 aattgagact aattgttctt ccatccactg atggccttta tcgcgcccgg cagcttcatg
    56821 ccaggagaga taacaggttc tgctgttttg ttttaacggc agcggtaata cctgtaattc
    56881 taaggattca gcgaactctt cagccagcca acgcggcgca atagcgacca aatgcgtttg
    56941 cgacaccacg ctaagtacgc tcatcattgc catgccctga tacgcgatac tggcttgctt
    57001 atctaccgtg tcataccaag gttgactaaa tgacgcgaaa cgatcgagcg aaaccgccgc
    57061 atgttgttcg ttataaacat catgtttcag taacgggcct ttaattgttg gatgattttt
    57121 gctggctacc agcaccattt catctttaaa taatggcacg ctggtaaatt caggacgatg
    57181 gaagtcttca taactaatca caaactccgt ttcctgataa cgcagctgat gttcagtgtt
    57241 ctgatttaat gaagacttga acataacatg tatatttggc gcaatctgct caatgtgatt
    57301 ataaatctgc gaggtcagaa tgctgtctaa cgggctgcaa acacaaagat gaaatacacg
    57361 ttcactgctc gcgggttcaa aacctgaact aggcaattca ttttgtacta gttgcaatgc
    57421 ctggcgaact gaaccaaaaa gttgaaatgc gcgagcagtc ggttgaatac cacggccata
    57481 acgaacaaaa agctcgtcat taaacatcac cttcaggcgt gcaacagcgt tactgaccgc
    57541 aggttgcgac atacccagaa catgagcggc acgggtaatg ttttgctcct gcatcacggc
    57601 atcgaaaacg gttaataagt tgagatcgac catgcgtagc tgtggcttgc ttaactccgc
    57661 cgtctctgaa tgatctgttt gtacctctgg catacttaac tccactgtca cacttaactc
    57721 cctttccctt attggaatgc agagaaataa tcctgaagaa tatgatttat catgcatata
    57781 aaataagaaa aagcaaaatg agtaaaattc ggaaaacata aagatacggg ttctgataaa
    57841 aacagaatcc attgccgtgg aatgagtcat ttaccacagc ataataatcc ataatgtttt
    57901 tgttgcgaaa acaatctaat cataactacc gcgaatactc aatcatctac aaaatggatt
    57961 aaatgtgctt atttaataat taatttatat atttaatgca ttaattctta acattaattg
    58021 atcaataata ttcaccaaat caatatcaaa aaaaatcgca aaacatataa ttcaatacaa
    58081 atcatcagga taggttttgc aacgcgtgca ttttgtcccc tttttcctcg ttgattagat
    58141 gcaaaaattt atgctgaaat atgtcaaccg atgaaaagcg tcggtagtta agcagaaatt
    58201 aatatcgctt actttaacca ccgtagcaca attagctaat tttacggatg cagaactcac
    58261 gctggcggga cgtttttatt gcgtcagggt tgacatccgt ttttgtatcc agtaactcta
    58321 aaagcatatc gcattcatct ggagctgatt taatgactca catcgttcgc tttatcggtc
    58381 tactactact aaacgcatct tctttgcgcg gtagacgagt gagcggcatc cagcattaag
    58441 ccagcacgca gtcaaacaaa aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg
    58501 aggcgctcta aaagagacaa ggacccaaac catgagccag caagtcatta ttttcgatac
    58561 cacattgcgc gacggtgaac aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca
    58621 aattgcgctg gcccttgagc gtatgggtgt tgacgtgatg gaagtcggtt tccccgtctc
    58681 ttcgccgggc gattttgaat cggtgcaaac catcgcccgc caggttaaaa acagccgcgt
    58741 atgtgcgtta gctcgctgcg tggaaaaaga tatcgacgtg gcggccgaat ccctgaaagt
    58801 cgccgaagcc ttccgtattc atacctttat tgctacctcc ccaatgcaca tcgccaccaa
    58861 gctgcgcagc accctggacg aggtgatcga acgcgctatc tatatggtga aacgcgcccg
    58921 taattacacc gatgatgttg aattttcttg cgaagatgcc gggcgtacac ccattgccga
    58981 tctggcgcga gtggtcgaag cggcgattaa tgccggtgcc accaccatca acattccgga
    59041 caccgtgggc tacaccatgc cgtttgagtt cgccggaatc atcagcggcc tgtatgaacg
    59101 cgtacctaac atcgacaaag ccattatttc cgtacatacc cacgacgatt taggcctggc
    59161 ggtcggcaac tcactggcgg cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa
    59221 cgggatcggc gagcgtgccg gtaactgttc gctggaagaa gtcatcatgg cgattaaagt
    59281 tcgtaaggat attctcaacg tccacaccgc cattaatcac caggagatat ggcgcaccag
    59341 ccagttagtt agccagattt gtaatatgcc gatcccggca aacaaagcca ttgttggcag
    59401 cggcgcattc gctcactcct ccggtatcca ccaggatggc gtgctgaaaa accgcgaaaa
    59461 ctacgaaatc atgacaccag aatctattgg tctgaaccaa atccagctga atctgacctc
    59521 tcgttcgggg cgtgcggcgg tgaaacatcg catggatgag atggggtata aagaaagtga
    59581 atataattta gacaatttgt acgatgcttt cctgaagctg gcggacaaaa aaggtcaggt
    59641 gtttgattac gatctggagg cgctggcctt catcggtaag cagcaagaag agccggagca
    59701 tttccgtctg gattacttca gcgtgcagtc tggctctaac gatatcgcca ccgccgccgt
    59761 caaactggcc tgtggcgaag aagtcaaagc agaagccgcc aacggtaacg gtccggtcga
    59821 tgccgtctat caggcaatta accgcatcac tgactataac gtcgaactgg tgaaatacag
    59881 cctgaccgcc aaaggtcacg gtaaagatgc gctgggtcag gtggatattg tcgccaacta
    59941 caacggtcgc cgcttccacg gcgtcggcct ggccaccgat attgtcgagt cctccgccaa
    60001 agccatggtg cacgtactga acaatatctg gcgtgccgca gaagtcgaaa aagagttgca
    60061 acgcaaagct caacacaacg aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat
    60121 attgccgtat tgccggggga cggtattggt ccggaagtga tgacccaggc gctgaaagtg
    60181 ctgaatgccg tgcgcaaccg ctttgcgatg cgcatcacca ccagccatta cgatgtaggc
    60241 ggcgcagcca ttgataacca cgggcaacca ctgccgcctg cgacggttga aggttgtgag
    60301 caagccgatg ccgtgctgtt tggctcggta ggcggcccga agtgggaaca tttaccacca
    60361 gaccagcaac cagaacgcgg cgcgctgctg ccgctgcgta agcacttcaa attattcagc
    60421 aacctgcgcc cggcaaaact gtatcagggg ctggaagcat tctgtccgct gcgtgcagac
    60481 attgccgcta acggcttcga catcctgtgc gtgcgcgaac tgaccggcgg catctatttc
    60541 ggtcagccaa aaggccgcga aggtagcgga caatatgaaa aagcgtttga taccgaggtg
    60601 tatcaccgtt ttgagatcga gcgtattgcc cgcatcgcgt ttgaatctgc tcgtaaacgt
    60661 cgccacaaag tcacctcaat cgacaaagct aacgtgctgc aatcctctat tttatggcgt
    60721 gaaatcgtca acgagatcgc cacggaatac ccggatgtcg aactggcgca tatgtacatc
    60781 gacaacgcca ccatgcagct gattaaagat ccatcacagt ttgacgttct gctgtgctcc
    60841 aacctgtttg gcgacattct gtctgacgag tgcgcaatga tcactggctc gatgggcatg
    60901 ttgccttccg ccagcctgaa cgagcaaggt tttggactgt atgaaccggc gggcggctcg
    60961 gcaccagata tcgcaggcaa aaacatcgcc aacccgattg cacaaatcct gtcgctggca
    61021 ctgctgctgc gttacagcct ggatgccgat gatgcggctt ccgccattga acgcgccatt
    61081 aaccgtgcat tagaagaagg cattcgcacc ggggatttag cccgtggcgc tgccgccgtt
    61141 agtaccgatg aaatgggcga tatcattgcc cgctatgtgg cagaaggggt gtaatcatgg
    61201 ctaaaacgtt atacgaaaaa ttattcgacg ctcacgtagt gtacgaagcc gaaaacgaaa
    61261 ccccgctgtt atatatcgac cgccacctag tgcatgaagt gacctcaccg caggcgttcg
    61321 atggtctgcg cgcccacggt cgcccggtac gtcagccggg caaaaccttc gccactatgg
    61381 atcacaacgt ctctacccag accaaagaca ttaatgcctg tggtgaaatg gcgcgcatcc
    61441 agatgcagga gctgattaaa aactgcaaag aatttggcgt cgagctgtat gacctgaatc
    61501 acccgtatca ggggatcgtc cacgtaatgg ggccggaaca gggcgtcacc ttgccgggga
    61561 tgaccattgt ctgcggcgac tcgcataccg ccacccacgg cgcgtttggc gcactggcct
    61621 ttggtatcgg cacttccgaa gttgaacacg tactggcaac acaaaccctg aaacagggcc
    61681 gtgcgaagat catgaaaatt gaagtccagg gcaaagccgc gccgggcatt accgcaaaag
    61741 atatcgtgct ggcaattatc ggtaaaactg gcagcgcagg cggcaccggg catgtggtgg
    61801 agttttgcgg cgaagcaatt cgtgatttaa gcatggaagg tcgtatgacc ctgtgcaata
    61861 tggcgatcga aatgggcgca aaagccggtc tggttgctcc tgacgaaacc acctttaact
    61921 atgtcaaagg ccgtctgcat gcgccgaaag gcaaagattt cgacgacgcc gttgcctact
    61981 ggaaaaccct gcaaaccgac gaaggcgcaa ctttcgatac cgttgtcact ctgcaagcag
    62041 aagaaatttc accgcaggtc acctggggca ccaatcccgg ccaggtgatt tccgtgaacg
    62101 acaatattcc cgatccggct tcgtttgccg atccggttga acgtgcgtcg gcagaaaaag
    62161 cgctggccta tatggggctg aaaccgggta ttccgctgac cgaagtggct atcgacaaag
    62221 tgtttatcgg ttcctgtacc aactcgcgca ttgaagattt acgcgcggca gcggagatcg
    62281 ccaaagggcg aaaagtcgcg ccaggcgtgc aggcactggt ggttcccggc tctggcccgg
    62341 taaaagccca ggcggaagcg gaaggtctgg ataaaatatt tattgaagcc ggttttgaat
    62401 ggcgcttgcc tggctgctca atgtgtctgg cgatgaacaa cgaccgtctg aatccgggcg
    62461 aacgttgtgc atccaccagc aaccgtaact ttgaaggccg ccaggggcgc ggcgggcgca
    62521 cgcatctggt cagcccggca atggctgccg ctgctgctgt gaccggacat ttcgccgaca
    62581 ttcgcaacat taaataagga gcacaccatg gcagagaaat ttatcaaaca cacaggcctg
    62641 gtggttccgc tggatgccgc caatgtcgat accgatgcaa tcatcccgaa acagtttttg
    62701 cagaaagtga cccgtacggg ttttggcgcg catctgttta acgactggcg ttttctcgat
    62761 gaaaaaggcc aacagccaaa cccggacttc gtgctgaact tcccgcagta tcagggcgct
    62821 tccattttgc tggcacgaga aaacttcggc tgtggctcct cgcgtgagca cgcgccctgg
    62881 gcattgaccg actacggttt taaagtggtg attgcgccga gttttgctga catcttctac
    62941 ggcaatagct ttaacaacca gctgctgccg gtgaaattaa gcgatgcaga agtggacgaa
    63001 ctgtttgcgc tggtgaaagc caatccgggg atccatttcg acgtggatct ggaagcgcaa
    63061 gaggtgaaag cgggagagaa aacctatcgc tttaccatcg atgccttccg ccgccactgc
    63121 atgatgaatg gtctggacag catcggcctg accctgcagc acgacgacgc tattgcgtct
    63181 tatgaagcaa aacaacctgc gtttatgaat tagacccctt gcccggtcaa atgaccgggc
    63241 tttccgctat cgtccacgtc atcaaacgtc tttaaccttt gcggttaaaa ataatgcgac
    63301 aacagaaata atcgcaatta cccagtagac agcaaagtgc ccccaacttt gtgcaattgc
    63361 tccctgaata acaccagcca gaattacccc ggtagagata gtgttggtaa ataaggtggt
    63421 agctgccccc gctcttccag gcattaaatc ctgaaaccat agcatcccaa tacccgcaac
    63481 aatgccgata aatacagcgt taaaaagttg cagcgtcatc aacgccatac ggctatgaaa
    63541 gaaaatcaat ccagtgtaaa acagcactcc tgccaccact gcaatgacca tcattcgccg
    63601 cttaccataa cgtttgacat agtagccagc cagaatcatt gctggtattt ccagtccagc
    63661 tgccgtcccc atcaggaaac ccgccagttt gtctggcaat cctaaatcgc tactgatcca
    63721 caacggcata tcaataatgt acatagtgtt gcaggtccac attaacgtcg aggcgacaaa
    63781 taacatccgt acgttactat cctgccagtc accttgcatt gataaagcat tttccgacgg
    63841 cagttctacc cgcgccacag acggaagcat aaatgcaatc aataccagac tgagtgtgaa
    63901 tatcccggcg gcaatcgaaa acatcactgt aaagccgtaa ttcaacgcca gcataaaggc
    63961 cagcggtgga ccgattaccc atgccagaga aagctgcgca cgcatcaccg agctaaacat
    64021 caccacttct cgcgccgagt tatccgcgta ttcccgcgcc agagcaaata actgtggcat
    64081 tgccgtattg gccagagatg ccagaagcac accacaggtg ataagcgtca gataatgacg
    64141 attaaatgca aacagtagcg cattgccaat agccatcaaa cagcaaaata taatcagttt
    64201 tcgccgatcg ccctgactgt cagaacgttt tgccaaccag aggcttacgc cgatcccagc
    64261 aatagcattc accgtataaa agagtccgat ccagaaaggt tgcgcgccaa cctcacgact
    64321 cagaaataag ctcaatgtag gagcctgtag cgccccggcc acccccatca taaaagcgac
    64381 cagcataaat gccgcgtaaa caccgttcat acggcgagcc atcgtcatta tccagatcat
    64441 acgttccctt tttagcgcgg cgagaataaa aaaaaccagc aggtataatc tgctggcggg
    64501 tgattttaca ccaatactca gtcacacatg atgcaggcaa gtcaactttc agaattgcgg
    64561 tcatcccact gcatcagtcc ttcaagcatg gttaatcgtt gtgggacgct taaccaacgc
    64621 aaccagcaca acttcgctgt cgcggtaaaa tagtgctgat aaaactgacg catggggcac
    64681 ctccttgctt catcgttagg aattattgac ttaatatagg gaaaataaaa ttgctgtctt
    64741 ttgcgcagga gttccccttt tatgccatct gctcgtctgc aacaacagtt catccgcctg
    64801 tggcaatgct gcgagggtaa atcgcaggac acaacgctca acgaactggc agcgttattg
    64861 agctgctcgc gtcgtcatat gcgtaccctg ctcaacacca tgcaggatcg cggctggctg
    64921 acgtgggaag cggaagtcgg gcgcggtaaa cgctcgcgtc tgacattcct ctataccggg
    64981 ctggcgcttc agcaacagcg ggcggaagac ctgctggagc aggatcgtat cgaccaacta
    65041 gtgcagttgg ttggcgacaa agcgactgtg cggcaaatgc tggtttctca tctgggccgc
    65101 agcttccgcc aggggcggca catcctgcgc gtgctctact atcgtccgtt gcgtaatctg
    65161 ctacctggca gcgcattgcg ccgttccgaa acccatattg cccggcaaat cttcagttcg
    65221 ctaacgcgca taaatgagga aaatggggaa ctggaagcag acatcgccca ccactggcag
    65281 caaatatcac cgcttcactg gcgtttcttt ttgcgtccag gagtccattt tcatcatggt
    65341 cgtgaactgg aaatggacga cgtgatcgcc tctttaaaac gaatcaatac gctgccgctc
    65401 tattcgcata ttacggacat tgtgtcgccg acgccctgga cgctggatat ccatctcacg
    65461 cagccggacc gctggttgcc gttactgctg gggcaagttc cggcgatgat cctgccgtgc
    65521 gaatgggaaa ccctcagtaa ctttgccagc catcctattg gcaccggtcc gtatgcggtg
    65581 attcgcaaca gcaccaatca actgaaaatt caggcattcg atgacttctt cggttaccgg
    65641 gcattaatcg acgaagttaa cgtctgggtt ctgccggaaa ttgccgacga gccagccggt
    65701 ggtctgatgc taaaaggtcc acagggcgag gaaaaagaga ttgaaagccg cctggaggaa
    65761 ggttgctact atttactgtt cgacagccgc acccatcgcg gggcgaatca gcaagtcagg
    65821 gactgggtaa gctatgtgct ttctccaact aatctggtct atttcgctga ggaacagtac
    65881 cagcaactgt ggttcccggc ttatggactg ctcccccgtt ggcaccatgc ccgcaccata
    65941 aagagcgaaa aaccggctgg cctggaaagc ctcaccctga ccttttatca ggatcacagt
    66001 gagcatcggg tgattgccgg gatcatgcag cagattctgg caagtcacca ggtcacgctg
    66061 gaaatcaaag agatcagcta cgatcagtgg catgaaggag agatcgagag cgatatctgg
    66121 cttaatagcg ccaactttac gctgccgctg gatttttcgc tgttcgcgca cttgtgcgag
    66181 atgccgctgc tccaacactg tattccgatc gactggcaag ccgacgccgc ccgctggcgc
    66241 aatggcgaaa tgaacctggc gaactggtgc cagcaactgg tcgccagtaa agcaatggtg
    66301 ccgcttatcc accactggct gatcattcag ggacaacgca gtatgcgcgg cctgcgtatg
    66361 aataccctcg gctggttcga ttttaaatca gcgtggtttg cgccaccgga tccatgattg
    66421 ctggtagata acaaaatcac tacactaacg ccgttctcaa cggggtgcca cgcaaacgcg
    66481 tgcgctgaga aaatacccgt cgaacctgat ccggataacg ccggcgaagg gatttgaggc
    66541 tccttctcaa gtcctttgcc actctttttt gaggtgcaaa gtgttaaaaa aatgtcttcc
    66601 cctgctgttg ctgtgcacag cgcccgtttt cgctaaaccc gttctgactg tttataccta
    66661 cgattccttc gccgccgact gggggccagg tccaaagatc aaaaaagctt ttgaagccga
    66721 ctgtaactgc gaactgaaac tggtggcgct ggaagatggc gtttcgcttc tcaaccgtct
    66781 acggatggaa ggcaaaaata gtaaagccga tgtggtgctg gggctggata acaatctgtt
    66841 agacgccgcc agtaaaaccg gactatttgc caaaagcggt gtggcagcgg atgccgttaa
    66901 cgttcccggc ggctggaata atgacacttt cgtcccgttt gattacggct atttcgcctt
    66961 cgtctatgac aagaacaaac tgaaaaaccc gccgcaaagc ctgaaagaat tggttgagag
    67021 cgatcagaac tggctggtga tttatcagga tccgcgtacc agcacgccgg ggctgggtct
    67081 gctgttgtgg atgcaaaaag tctatggcga tgacgcccca caagcctggc aaaaactggc
    67141 gaagaaaacc gtcacggtca ccaaaggctg gagcgaagcc tacggcctgt ttttaaaagg
    67201 tgaaagcaat ctggtactga gttacaccac ctctccggct tatcacattc tcgaagagaa
    67261 aaaagataac tacgccgccg cgaactttag cgaaggtcac tatctgcagg tggaagtcgc
    67321 cgcccgcacc gctgccagca agcagccgga gctggcgcaa aaattcctcc agtttatggt
    67381 ttctccggct tttcagaatg cgatcccaac cggtaactgg atgtatccgg tggcaaatgt
    67441 cacgctgcct gccgggtttg aacaattgac caaaccggcc accacgctgg agttcacacc
    67501 agctgaagtg gcggcacaac gtcaggcatg gattagcgaa tggcaacgcg ccgtcagccg
    67561 ttaattcccg gctggttaat tccaggtgta agcgccgcca cgctggtggt agcggttgcg
    67621 atcgcggcgt ttctcgccct gtggtggaac gcgccgcagg gtaactgggt ggcagtctgg
    67681 caggacagct acctgtggca tgtggtgcgc ttctccttct ggcaggcgtt tctctcggcg
    67741 ctactctctg tcgtacccgc gatattcctc gcccgcgccc tctatcgcag gcgctttccg
    67801 ggtcggctgg cgctgttgcg tctgtgcgca atgaccttga tcctcccggt gctagttgct
    67861 gttttcggca ttcttagcgt ctatggtcgt cagggctggc tagcatcgct ctgccaatcg
    67921 ctcggtctgg agtggacctt ttcgccctac ggcctgcaag gcattttgct ggcgcacgta
    67981 ttttttaatc tgccgatggc gagccgctta ttactccagg cactggaaaa cattcccggc
    68041 gaacaacgtc agcttgccgc ccagcttggg atgcgcggct ggcatttttt ccgcttcgtc
    68101 gaatggccgt ggctacggcg acaaatcccg ccggttgccg cgcttatctt tatgctctgt
    68161 ttcgccagct tcgccaccgt gctatcgctg ggcggcggtc cgcaggcaac cactatcgag
    68221 ctggctattt atcaggcgct gagttacgac tacgatcctg cccgcgcggc gatgctggcg
    68281 ctgctccaga tggtgtgttg ccttgggctg gtgctgttga gtcagcgact aagtaaggcc
    68341 attgcgcccg gcaccacgct gctgcaaggc tggcgcgatc cggacgatcg tctgcatagc
    68401 cgcatttgcg acacggtgtt aatagtgctg gcgctgctgc tgttgctgcc accgttgctg
    68461 gcggtgatcg tcgatggggt aaatcgccag ttgccggaag tgctggcaca accggtgctg
    68521 tggcaggcgc tgtggacctc gttgcgtatt gcgctggcgg caggtgtatt gtgcgtagtg
    68581 ctgaccatga tgctgctatg gagcagtcgc gaactgcggg cgcggcagaa aatgctggcg
    68641 ggtcaggcgc tggagatgag cggcatgttg atcctcgcca tgccggggat tgtgctggct
    68701 accggcttct ttttactgct caacaacact atcggcctgc cacaatctgc tgacggcatt
    68761 gtgattttca ccaatgcgtt aatggcgatc ccttatgcgc tgaaagtact ggaaaacccg
    68821 atgcgcgata tcaccgcccg ctacagtatg ttgtgtcagt cgctgggcat tgaaggctgg
    68881 tcgcgcttaa aagtggtcga gctgcgcgcc ctgaaacgtc cactggcgca ggcgctggct
    68941 tttgcctgcg tgctgtcgat tggtgatttt ggcgtagtgg cgttgttcgg taacgatgat
    69001 ttccgtaccc tgccgtttta tctctaccag caaattggct cctatcgcag ccaggacggc
    69061 gcggtcaccg cgttaattct gctactactc tgttttctgc tgtttaccgt gattgaaaaa
    69121 ctaccggggc gaaatgttaa aactgactga tatcacctgg ctttatcacc atttgccgat
    69181 gcgttttagc ttaacggtag aacgcggcga gcaggtggca atcctcgggc caagcggcgc
    69241 gggtaaaagt accctgctga atttgattgc cggttttctg acgccagcca gcggttcgct
    69301 gactatcgat ggcgttgatc acaccacgac gccgccgtca cgccgtccgg tgtcgatgct
    69361 gtttcaggag aacaacctgt tcagccacct gacggtcgcg cagaacatcg ggctggggct
    69421 aaatccgggg ttgaaactga acgcggcaca gcaggagaaa atgcacgcga ttgcccgtca
    69481 gatggggatt gataatttaa tggcgcggtt accgggcgag ctttccggcg gtcagcgaca
    69541 gcgcgtggcg ttagcgcgtt gtctggtgcg cgaacagccg attttattgc tcgatgaacc
    69601 gttctctgcg ctcgatccgg cgttacgtca ggagatgctg acgctggtga gcacgagctg
    69661 ccagcagcaa aaaatgacgc tattgatggt gtcgcacagt gtggaagatg cggcgcggat
    69721 cgccacacgc tcggtggtgg tcgccgacgg gcgcatcgcc tggcagggta agactgaaga
    69781 attgttgagc ggtaaggcga gtgcttcggc aatattgggg attacgggtt agtatgcact
    69841 gtcagatgcg gcgtgaacac cttatccgac ctacggacag tgcgttttgt aggcctgata
    69901 agacgcggtc agcgtcgcat gaggcaaccc gcacaagaca atcccctaaa ccccaaccac
    69961 tttacgcaaa atatcgatat acaccggcat caacgggtgg cgaattaaca ccaccagcgc
    70021 caccacgccg atggcagaaa tcaacggcgt cagccacaac aaacggccgc gcgacaaata
    70081 atgacttaaa cgatcggttg ctttaccgct gcgccataac cgccagcaca gccagccacc
    70141 aacccacaaa aataccgccg tcgccagcag caaccattta aactcgccgc tctgcattcc
    70201 ggctggaata tcgatcgccg cgcccgccag aatcccaggc aggaagtaaa acggtggcca
    70261 cagcaggcag ccgataatat tcggcgtaat gaattttgcc accggcagat ccagcattcc
    70321 cgccaccatt ggcaccagcg gacgcgtcgg gccaacaaag cgaccgacca gaatggtgaa
    70381 catgctgtgt tgatgcaacg catgttcagt tttatcgagt aatgctttgt ttttcttcag
    70441 aaatgaccag cgatgcaacg gctttttaaa acgccagccc agccagaagg agatccagtc
    70501 gcccagcaga caaccgacaa tccctgccag ccaggcgtgc cagaaactta actcgccgct
    70561 gccaatcagc gctcccagcc ccgccatcag caccgtaccg ggtagaatca aaccgaccag
    70621 cgccagcgac tccagaaagg ccaccaacac caccgccatc aatgaataca cggtagattg
    70681 ggtaataaag tgttccagca atgcttgcat aatgtgcctg tcaaatggac gaagcaggga
    70741 ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt actaattatg
    70801 acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc
    70861 tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat
    70921 tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga
    70981 tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg
    71041 acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt
    71101 ctgccaggtg atcgctgatg tactgacaag cctcgcgtac acgattatcc atcggtggat
    71161 ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg
    71221 ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt
    71281 cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa acccgtattg gcaaatattg
    71341 acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat
    71401 accattcgcg agcctccgga tgacaaccgt agtgatgaat ctctcctggc gggaacagca
    71461 aaatatcacc cggtcggcag acaaattctc gtccctgatt tttcaccacc ccctgaccgc
    71521 gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga
    71581 gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc
    71641 ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag
    71701 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
    71761 tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
    71821 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
    71881 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
    71941 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttgga
    72001 tggagtgaaa cgatggcgat tgcaattggc ctcgattttg gcagtgattc tgtgcgagct
    72061 ttggcggtgg actgcgccag cggtgaagag atcgccacca gcgtagagtg gtatccccgt
    72121 tggcaaaaag ggcaattttg tgatgccccg aataaccagt tccgtcatca tccgcgtgac
    72181 tacattgagt caatggaagc ggcactgaaa accgtgcttg cagagcttag cgtcgaacag
    72241 cgcgcagctg tggtcgggat tggcgttgac agtaccggct cgacgcccgc accgattgat
    72301 gccgacggta acgtgctggc gctgcgcccg gagtttgccg aaaacccgaa cgcgatgttc
    72361 gtattgtgga aagaccacac tgcggttgaa gaagcggaag agattacccg tttgtgccac
    72421 gcgccgggca acgttgacta ctcccgctat attggcggta tttattccag cgaatggttc
    72481 tgggcaaaaa tcctgcatgt gactcgccag gacagcgccg tggcgcaatc tgccgcatcg
    72541 tggattgagc tgtgcgactg ggtgccagct ctgctttccg gtaccacccg cccgcaggat
    72601 attcgtcgcg gacgttgcag cgccgggcat aaatctctgt ggcacgaaag ctggggcggc
    72661 ttgccgccag ccagtttctt tgatgagctg gacccgatcc tcaatcgcca tttgccttcc
    72721 ccgctgttca ctgacacctg gactgccgat attccggtgg gcaccttatg cccggaatgg
    72781 gcgcagcgtc tcggcctgcc tgaaagcgtg gtgatttccg gcggcgcgtt tgactgccat
    72841 atgggcgcag ttggcgcagg cgcacagcct aacgcactgg taaaagttat cggtacttcc
    72901 acctgcgaca ttctgattgc cgacaaacag agcgttggcg agcgggcagt taaaggtatt
    72961 tgcggtcagg ttgatggcag cgtggtgcct ggatttatcg gtctggaagc aggccaatcg
    73021 gcgtttggtg atatctacgc ctggtttggt cgcgtactcg gctggccgct ggaacagctt
    73081 gccgcccagc atccggaact gaaagcgcaa atcaacgcca gccagaaaca actgcttccg
    73141 gcgctgaccg aagcatgggc caaaaatccg tctctggatc acctgccggt ggtgctcgac
    73201 tggtttaacg gtcgtcgcac gccaaacgct aaccaacgcc tgaaaggggt gattaccgat
    73261 cttaacctcg ctaccgacgc tccgctgctg ttcggcggtt tgattgctgc caccgccttt
    73321 ggcgcacgcg caatcatgga gtgctttacc gatcagggga tcgccgtcaa taacgtgatg
    73381 gcgctgggcg gcatcgcgcg gaaaaaccag gtcattatgc aggcctgctg cgacgtgctg
    73441 aatcgcccgc tgcaaattgt tgcctctgac cagtgctgtg cgctcggtgc ggcgattttt
    73501 gctgccgtcg ccgcgaaagt gcacgcagac atcccatcag cccagcaaaa aatggccagt
    73561 gcggtagaga aaaccctgca accgcgcagc gaacaggcac aacgctttga acagctttat
    73621 cgccgctatc agcaatgggc gatgagcgcc gaacaacact atcttccaac ttccgccccg
    73681 gcacaggctg cccaggccgt tgcgactcta taaggacacg ataatgacga tttttgataa
    73741 ttatgaagtg tggtttgtca ttggcagcca gcatctgtat ggcccggaaa ccctgcgtca
    73801 ggtcacccaa catgccgagc acgttgttaa tgcgctgaat acggaagcga aactgccctg
    73861 caaactggtg ttgaaaccgc tgggcaccac gccggatgaa atcaccgcta tttgccgcga
    73921 cgcgaattac aacgatcgtt gcgctggtct ggtggtgtgg ctgcacacct tctccccggc
    73981 caaaatgtgg atcaacggcc tgaccatgct caacaaaccg ttgctgcaat tccacaccca
    74041 gttcaacgcg gcgctgccgt gggacagtat cgatatggac tttatgaacc tgaaccagac
    74101 tgcacatggc ggtcgcgagt tcggcttcat tggcgcgcgt atgcgtcagc aacatgccgt
    74161 cgttaccggt cactggcagg ataaacaagc ccatgagcgt atcggctcct ggatgcgtca
    74221 ggcggtctct aaacaggata cccgtcatct gaaagtctgc cgatttggcg ataacatgcg
    74281 tgaagtggcg gtcaccgatg gcgataaagt tgccgcacag atcaagttcg gtttctccgt
    74341 caatacctgg gcggttggcg atctggtgca ggtggtgaac tccatcagcg acggcgatgt
    74401 taacgcgctg gtcgatgagt acgaaagctg ctacaccatg acgcctgcca cacaaatcca
    74461 cggcgaaaaa cgacagaacg tgctggaagc ggcgcgtatt gagctgggga tgaagcgttt
    74521 cctggaacaa ggtggcttcc acgcgttcac caccaccttt gaagatttgc acggtctgaa
    74581 acagcttcca ggtctgcccg tacagcgtct gatgcagcag ggttacggct ttgcgggcga
    74641 aggcgactgg aaaaccgccg ccctgcttcg catcatgaag gtgatgtcaa ccggtctgca
    74701 gggcggcacc tcctttatgg aggactacac ctatcacttc gagaaaggta atgacctggt
    74761 gctcggctcc catatgctgg aagtctgccc gtcgattgcc gtagaagaga aaccgatcct
    74821 cgacgttcag catctcggta ttggtggtaa ggacgatcct gcccgactga tcttcaatac
    74881 ccaaaccggt ccagcgattg tcgccagcct gattgatctc ggcgatcgtt accgtctgct
    74941 ggttaactgt atcgacacgg tgaaaacacc gcactccctg ccgaaactgc cggtggcgaa
    75001 tgcactgtgg aaagcgcaac cggatctgcc aactgcttcc gaagcgtgga tcctcgctgg
    75061 tggcgcgcac cataccgtct tcagccatgc gctgaacctc aacgatatgc gccagttcgc
    75121 cgagatgcac gacattgaaa tcacggtgat tgataacgac acccgcctgc cagcgtttaa
    75181 agacgcactg cgctggaacg aagtgtatta cggatttcgt cgctaagtag ccacatcagg
    75241 tatgtaacgc ctgatgcgac gctgacgcgt cttatctggc ctacacgctg cgattttgta
    75301 ggccggataa gcaaagcgca tccggcattc aacgcctgat gcgacgctgg cgcgtcttat
    75361 caggcctacg cgctgcgatt ttgtagaccg gataagcgaa gcgcatccgg cacgaaggag
    75421 tcaacatgtt agaagatctc aaacgccagg tattagaagc caacctggcg ctgccaaaac
    75481 acaacctggt cacgctcaca tggggcaacg tcagcgccgt tgatcgcgag cgcggcgtct
    75541 ttgtgatcaa accttccggc gtcgattaca gcgtcatgac cgctgacgat atggtcgtgg
    75601 ttagcatcga aaccggtgaa gtggttgaag gtacgaaaaa gccctcctcc gacacgccaa
    75661 ctcaccggct gctctatcag gcattcccct ccattggcgg cattgtgcat acgcactcgc
    75721 gccacgccac catctgggcg caggcgggtc agtcgattcc agcaaccggc accacccacg
    75781 ccgactattt ctacggcacc attccctgca cccgcaaaat gaccgacgca gaaatcaacg
    75841 gcgaatatga gtgggaaacc ggtaacgtca tcgtagaaac ctttgaaaaa cagggtatcg
    75901 atgcagcgca aatgcccggc gttctggtcc attcccacgg cccgtttgca tggggcaaaa
    75961 atgccgaaga tgcggtgcat aacgccatcg tgctggaaga ggtcgcttat atggggatat
    76021 tctgccgtca gttagcgccg cagttaccgg atatgcagca aacgctgctg gataaacact
    76081 atctgcgtaa gcatggcgcg aaggcatatt atgggcagta atgactgtat aaaaccacag
    76141 ccaatcaaac gaaactaggc tatactcaag cctggttttt tgatggattt tcagcgtggc
    76201 gcaggcaggt tttatcttaa cccgacactg gcgggacacc ccgcaaggga cagaagtctc
    76261 cttctggctg gcgacggaca acgggccgtt gcaggttacg cttgcaccgc aagagtccgt
    76321 ggcgtttatt cccgccgatc aggttccccg cgctcagcat attttgcagg gtgaacaagg
    76381 ctttcgtctg acaccgctgg cgttaaagga ttttcaccgc cagccggtgt atggccttta
    76441 ctgtcgtgcc catcgccaat tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac
    76501 cgtctacgag gccgatgtgc gcccgccaga acgctatctg atggagcggt ttatcacctc
    76561 accggtgtgg gtcgagggtg atatgcacaa tggcactatc gttaatgccc gtctgaaacc
    76621 gcatcccgac tatcgtccgc cgctcaagtg ggtttctata gatattgaaa ccacccgcca
    76681 cggtgagctg tactgcatcg gcctggaagg ctgcgggcag cgcatcgttt atatgctggg
    76741 gccggagaat ggcgacgcct cctcgcttga tttcgaactg gaatacgtcg ccagccgccc
    76801 gctgctgctg gaaaaactca acgcctggtt tgcaaaccac gatcccgatg tgatcatcgg
    76861 ctggaacgtg gtgcagttcg atctgcgaat gctgcaaaaa catgccgagc gttaccgtct
    76921 tccgctgcgt cttgggcgcg ataatagcga gctggagtgg cgcgagcacg gctttaaaaa
    76981 cggcgtcttt tttgcccagg ctaaaggtcg gctaattatc gacggtatcg aggcgctgaa
    77041 atccgcgttc tggaatttct cttcattctc gctggaaact gtcgctcagg agctattagg
    77101 cgaaggaaaa tctatcgata acccgtggga tcgaatggac gaaattgacc gccgtttcgc
    77161 cgaagataaa cctgcgctgg caacttataa cctgaaagat tgcgagctgg tgacgcagat
    77221 cttccacaaa actgaaatca tgccattttt actcgaacga gcaacggtga acggcctgcc
    77281 ggtggatcgg cacggcggtt cggtggcagc gtttggtcat ctctattttc cgcgaatgca
    77341 tcgcgctggt tatgtcgcgc ctaatctcgg cgaagtgccg ccgcacgcca gccctggcgg
    77401 ctacgtgatg gattcacggc cagggcttta tgattcagtg ctggtgctgg actataaaag
    77461 cctgtacccg tcgatcatcc gcacctttct gattgatccc gtcgggctgg ttgaaggcat
    77521 ggcgcaacct gatccagagc acagtaccga aggttttctc gatgcctggt tctcacggga
    77581 gaaacattgc ctgccggaga ttgtgactaa catctggcac gggcgcgatg aagccaaacg
    77641 tcagggtaat aagccgcttt cgcaggcgct gaagatcatt atgaatgcct tttatggcgt
    77701 gctcggcacc accgcctgcc gcttcttcga tccgcgcctg gcatcgtcga tcaccatgcg
    77761 tggtcatcag atcatgcggc agaccaaagc attgattgaa gcgcagggct acgacgttat
    77821 ctacggagat accgactcga cttttgtctg gctaaaaggt gcacattcgg aagaagaggc
    77881 ggcgaaaatc ggtcgtgcac tggtgcagca cgttaacgcc tggtgggcgg aaacgctgca
    77941 aaaacaacgg ctgaccagcg cattagaact ggagtatgaa actcatttct gccgttttct
    78001 tatgccaacc attcgcggag ctgataccgg cagtaaaaag cgctacgccg ggctgattca
    78061 ggagggcgat aagcagcgga tggtgtttaa agggctggaa accgtgcgca ccgactggac
    78121 gccgctggcc cagcagtttc agcaggagct gtacctgcgt atcttccgca acgagccata
    78181 tcaggagtac gtccgccaaa ccatcgacaa actgatggcg ggtgaactgg atgcgcggct
    78241 ggtttaccgt aaacgccttc gccgtccgct gagcgaatat cagcgtaatg ttccgcctca
    78301 tgtacgcgcc gctcgccttg ccgatgaaga aaatcaaaag cgcggtcgcc ccttgcaata
    78361 tcagaatcgc ggcaccatta agtacgtatg gaccaccaac ggcccggagc cgctggacta
    78421 ccaacgttca ccactggatt acgaacacta tctgacccgc cagctacaac ccgtggcgga
    78481 gggaatactc ccttttattg aggataattt tgctacactt atgaccgggc aacttgggct
    78541 attttgagca aaaaaaagag ttcgccagat accattttga tgcgtgacga atgctttgcc
    78601 atccagtacc atagcgccct ttccattcct ggacctgaat aacaccacta cctcataagc
    78661 acggtagcgg gtggttattg cctgcaatta aagatataga gccgaacaca tatgcctttt
    78721 acacttggtc aacgctggat cagcgataca gaaagcgaat tgggacttgg aaccgttgtc
    78781 gcggtggatg cgcgaactgt cactttactt ttcccatcta ctggtgaaaa ccgtctgtac
    78841 gcacgcagtg attcccccgt gacccgcgtg atgttcaacc ctggtgatac cattaccagc
    78901 catgacggct ggcagatgca agtcgaagaa gtaaaagaag aaaatggctt gctgacctat
    78961 atcggtactc gcctggatac tgaagagtcc ggcgtagccc tgcgtgaagt tttccttgat
    79021 agcaaactgg tgttcagcaa accgcaggac cgtctgtttg ccgggcagat tgaccgtatg
    79081 gaccgctttg cgctgcgtta tcgcgcgcgt aagtattcca gcgaacagtt ccgtatgccg
    79141 tacagcggcc tgcgcggtca gcgtaccagc ctgatcccgc atcagctcaa catcgctcat
    79201 gatgttggtc gccgccacgc gccgcgcgtc ctgctggctg acgaagtggg tttagggaaa
    79261 accattgaag ccgggatgat cctgcatcag caactgctct ctggcgctgc tgaacgtgtg
    79321 ctgattatcg tcccggaaac cttacagcat cagtggctgg tagaaatgct gcgccgtttc
    79381 aacctgcgct ttgctctgtt tgatgatgag cgttatgccg aagctcagca cgatgcttac
    79441 aacccgtttg acaccgaaca gctggtgatt tgctcgctgg attttgcccg tcgtagcaaa
    79501 cagcgcctgg aacatctctg tgaagccgaa tgggacctgc tggtggtcga tgaagcgcat
    79561 cacctggtgt ggagcgaaga tgcgccaagc cgtgaatatc aggccattga acaactggca
    79621 gagcacgtgc cgggcgttct gctgctgacc gcgaccccgg aacagctggg gatggaaagc
    79681 cacttcgccc gtctgcgtct gctggacccg aaccgtttcc acgattttgc gcagttcgtt
    79741 gaagagcaga aaaattatcg tccggttgcg gacgccgttg ccatgctgct ggcaggtaac
    79801 aaactgagca atgacgaact gaacatgctc ggcgagatga tcggcgagca ggatatcgag
    79861 ccgctgttgc aggcagcaaa cagcgacagc gaagatgccc agagcgcccg tcaggagctg
    79921 gtttcgatgc tgatggatcg ccacggcacc agccgcgtgc tgttccgtaa cacgcgtaac
    79981 ggtgtgaaag gattcccgaa acgcgagctg cacaccatta agctgccgct accgacgcag
    80041 tatcagacgg ctattaaagt ctccggcatt atgggcgcac gtaaaagtgc ggaagatcgt
    80101 gctcgcgata tgctctaccc ggagcgtatt tatcaggaat ttgaaggtga taacgccacc
    80161 tggtggaact tcgatccgcg cgttgagtgg ctgatgggct acctgaccag ccatcgctct
    80221 cagaaagtgc tggtgatctg cgccaaagct gccactgcgc tgcaactgga gcaggtactg
    80281 cgcgaacgtg aaggtattcg cgctgcggtg ttccacgaag gtatgtcgat tatcgaacgt
    80341 gaccgcgctg ccgcctggtt tgccgaagaa gacaccggcg cacaggtact gctgtgctca
    80401 gaaatcggtt ctgaaggacg taactttcag ttcgccagcc acatggtgat gtttgacctg
    80461 ccattcaacc cggatctact ggagcagcgt attggtcgtc tggatcgtat cggccaggcg
    80521 cacgatattc agatccatgt gccttatctg gagaaaaccg ctcagtcggt gctggtgcgc
    80581 tggtatcacg aaggtctgga tgcatttgag cacacctgcc cgaccggacg cactatttac
    80641 gatagcgtat acaacgatct gattaactat ctggcttcac cggatcaaac cgaaggcttt
    80701 gacgatctga tcaaaaactg ccgcgagcaa catgaagcgc tgaaagcaca gctggaacag
    80761 ggtcgtgacc gcctgctgga aatccactcc aacggtggcg aaaaagccca ggcactggca
    80821 gaaagcattg aagagcagga tgacgatacc aacctgatcg ccttcgccat gaacctgttc
    80881 gatattatcg gtatcaatca ggacgatcgc ggcgacaaca tgatcgtgct gacgccgtcc
    80941 gatcatatgc tggtgccgga cttccctggc ctgtcggaag atggcatcac catcaccttt
    81001 gatcgtgaag tggcgctggc gcgtgaagat gcacagttta ttacctggga gcatccgctg
    81061 atccgcaacg gtctggatct gatcctttct ggcgataccg gtagcagcac gatttcactg
    81121 ttaaaaaaca aagcgttgcc agtaggtacg ctgttggtgg aactgattta tgtggttgaa
    81181 gcccaggctc cgaagcagtt gcagctcaac cgcttcctgc caccgacgcc ggtacgtatg
    81241 ctgctggata aaaacggcaa caacctggcg gcgcaggtag agtttgaaac ctttaaccgc
    81301 cagcttaacg cggttaaccg tcacaccggc agcaaactgg ttaacgccgt gcagcaggat
    81361 gttcacgcta tccttcaact gggtgaagcg cagatcgaga aatctgcccg tgcattgatt
    81421 gatgcagcgc gtaacgaagc cgacgaaaaa ctgtctgccg agctgtctcg tctggaagct
    81481 ctgcgtgcag tgaacccgaa cattcgtgac gacgaactga ccgccattga gagcaaccgt
    81541 cagcaggtaa tggaaagcct ggatcaggca ggttggcgtc tggatgccct gcgtttgatc
    81601 gttgtaacgc atcagtaacg gagtcgaaaa tggggatgga aaactacaat ccaccgcagg
    81661 aaccctggtt ggttatcctg tatcaggatg accatattat ggtggtcaac aagccgagcg
    81721 gtttgttgtc agtgccgggt cgtctggaag agcacaaaga cagcgtgatg acgcgcattc
    81781 agcgtgatta tccgcaggca gaatcggtgc atcgtctgga tatggctacc agcggcgtga
    81841 ttgtagtggc gctgaccaaa gccgcggagc gggagttaaa acgccagttc cgcgagcgcg
    81901 agccgaaaaa gcagtatgtg gcccgcgtct gggggcatcc atcccccgca gaaggtctgg
    81961 tggatctgcc gctgatttgc gactggccaa accgcccgaa acagaaagtc tgttacgaaa
    82021 cgggtaaacc tgcgcagacg gaatatgaag tggtggagta tgcggcggat aacacggcaa
    82081 gagtggtgtt aaaaccgatt accgggcgtt cgcatcaatt gcgtgtgcat atgctggcgc
    82141 tggggcatcc gattctcggc gatcgttttt atgcatcacc agaagcgaga gcgatggcac
    82201 cgcgtttgtt gctacatgca gagatgttga cgattaccca tccggcgtat ggcaatagta
    82261 tgacgtttaa agcgccagcg gatttttaat cgtcacgctg gcgcagttcg gtaggcggat
    82321 aaggcgttca cgccgcatcc ggcaattgtg cgccgattgc ctgatgcgac gcgggccgcg
    82381 tcttatcagg cctacagttt aaaacttatt taaacccttt ctgctgcttt atcagctcat
    82441 atgcctgctg aatttcctgc gctttctgct tcgccatctc catcatctca ggcggcaaac
    82501 ctttcgccac cagcttatcg ggatggtgtt cactcatcag cttacggtag gcacgtttga
    82561 tggtggtcgc atcatccgtc ggcttcacgc ccagcacatt acaggcatct tccagcgtcg
    82621 ggccacgctg cgcttgctgc cagttaccac cgccagattg ctgctgataa ccgccgccaa
    82681 actgtgcacc gccctgcatc atgcgcaaaa actggtcaaa ctgggcgcgg gagatcccta
    82741 attcttcagc aatgacatac agcaccgccc gttcattcgg gtgcagtgaa ccatcagcaa
    82801 acgccgcctg aatctggatc tccagaaaca tacgaattaa gtcaaaacga ccaaagcaga
    82861 cactgcgaaa ctggcgcatc ttttcgcgca gcgggtaatt atctgatttt cccacccgga
    82921 acgcgttttg cgccgcagta cgggaagcgc catgaaggtt cattcggtcc atcaactggc
    82981 tggcgatatg aatatcagcc tccgtgacgc gacctttgga tttggttaaa tgccccatca
    83041 cttcaaaagt ggtggcaaaa aacagcgcct gacgctcacg ctggttggcg aaccacgcca
    83101 ttttacggct acgggcttta tcaaacatat ggccaattaa caggcctaac actacgcccc
    83161 aaaagccgcc gcccatcagt aaggccacgg ccacgccaat gatttttccc caatactgca
    83221 tatattcccc aaatcgacac acggatatca gggctatctc ccacaatata aaggtgcttt
    83281 taccgttttc cggctgcggt caattgtggg acatcgccta taatttgcat tatcatacct
    83341 gtcattcaat gccgtgccta acaccactga cgctattcgg acaggattaa cactagcgta
    83401 gagatgacga gtacgttagt ctctgaccgt ttgtcacgcg caacgttacc gatgatggaa
    83461 caataaaatc aacgtatgaa aaaacgtatc cccactctcc tggccaccat gattgccacc
    83521 gccctttata gtcaacaggg actggcagcc gacctcgcct cacagtgcat gttgggcgtg
    83581 ccaagctatg accgtcctct ggtacagggc gataccaatg acttacccgt gactatcaat
    83641 gctgaccacg cgaaagggga ctacccggat gacgccgtgt ttactggcag cgtggatatc
    83701 atgcagggta acagccgtct gcaggccgac gaagtgcagc tccatcaaaa agaggcacta
    83761 ggacaaccgg agccggtacg taccgttgat gcgctcggta atgtccatta cgacgataac
    83821 caggtgatcc tcaaagggcc gaaaggctgg gcgaatctga acaccaaaga taccaacgtc
    83881 tgggaaggtg attaccagat ggtgggtcgc cagggtcgcg gtaaagcgga cctgatgaaa
    83941 caacgtggcg aaaaccgcta taccattctg gataacggta gctttacctc ctgtctgccg
    84001 ggttctgaca cctggagcgt ggtaggtagc gaaattattc atgaccgcga agaacaagtt
    84061 gcggagatct ggaacgcccg ctttaaggtg ggtccggtac cgatctttta tagcccctat
    84121 ttgcagttgc cggtgggtga caaacgtcgc tctggtttct tgatcccgaa cgccaagtac
    84181 accaccacca actactttga gttctacctg ccatattact ggaacatcgc gccaaatatg
    84241 gatgccacca tcacgccgca ttatatgcat cgtcgtggca acatcatgtg ggagaacgaa
    84301 ttccgctacc tctcccaggc tggcgctggc ttgatggaac tggactatct gccttcagat
    84361 aaagtctatg aagatgaaca cccgaacgat gacagttcac gtcgttggtt attctactgg
    84421 aaccactccg gggtcatgga tcaggtgtgg cgtttcaacg tcgactacac caaggtcagc
    84481 gatcctagct acttcaatga tttcgataac aagtacggtt ccagtactga cggctacgca
    84541 acgcaaaaat tcagcgttgg ctatgcggtg caaaacttca atgccaccgt ttcaaccaag
    84601 cagttccagg ttttcagcga acagaacacc agtagctact cggcagagcc gcagttagac
    84661 gttaattact accagaatga tgttggtccg tttgatacgc gtatttacgg ccaggcagtg
    84721 cactttgtta acaccagaga cgacatgcct gaagcaaccc gtgttcacct agaaccgacc
    84781 atcaatttgc ctctctctaa taactggggc agcatcaata ccgaagcgaa gtttctggca
    84841 acccattatc agcaaaccaa tcttgactgg tataactcca gaaacacgac caagctggac
    84901 gaatccgtta accgcgtaat gccgcaattc aaagttgacg gcaaaatggt ctttgaacgc
    84961 gatatggaaa tgctggctcc gggttatacc caaacgctgg aaccgcgcgc gcagtatttg
    85021 tacgtgccgt atcgcgatca gagcgacatc tataactacg actcgtctct gctgcaatct
    85081 gactactctg gcctgttccg ggaccggact tacggcggtc ttgaccgtat tgcctccgct
    85141 aaccaggtga cgaccggtgt cacatctcgc atatatgatg atgctgccgt tgaacgtttt
    85201 aatatttccg ttggtcaaat ctactatttc acggagtctc gcactggcga tgacaacata
    85261 acatgggaga atgacgacaa aacgggctca ctggtgtggg caggcgacac ctactggcgt
    85321 atctccgagc gttggggatt gcgtggcggg attcagtacg atacacgtct ggataacgta
    85381 gcgaccagta actccagcat tgaataccgt cgggatgaag accgtctggt acagctgaat
    85441 taccattacg ccagcccgga atatattcag gctacgctgc ctaagtacta ttccactgct
    85501 gagcaatata agaatggtat ttcgcaggta ggtgctgtcg ccagctggcc aattgccgat
    85561 cgttggtcca ttgttggggc ctactactac gacaccaatg cgaacaagca agccgactct
    85621 atgttaggtg tgcaatacag ctcctgctgc tatgcaattc gcgtcggtta cgagcggaag
    85681 ctgaacggtt gggataacga taaacaacat gcggtatatg acaacgcaat cggctttaac
    85741 atcgaacttc gcggcctgag ctccaactac ggtctgggta cgcaagagat gctgcgttcg
    85801 aacattctgc cgtatcaaaa cactttgtga tttgttgatt taccacgtaa tccgcagtgc
    85861 ggttaattga aatggaaaaa gtatgaagaa ctggaaaacg ctgcttctcg gtatcgccat
    85921 gatcgcgaat accagtttcg ctgcccccca ggtagtcgat aaagtcgcag ccgtcgtcaa
    85981 taacggcgtc gtgctggaaa gcgacgttga tggattaatg cagtcggtaa aactgaacgc
    86041 tgcgcaggca aggcagcagc ttcctgatga cacgacgctg cgccaccaaa tcatggaacg
    86101 tttgatcatg gatcaaatca tcctgcagat ggggcagaaa atgggagtga aaatctccga
    86161 tgagcagctg gatcaggcga ttgctaacat cgcgaaacag aacaacatga cgctggatca
    86221 gatgcgcggc cgtctggctt acgatggtct gaactacaac acttatcgta accagatccg
    86281 caaagagatg attatctctg aagtgcgtaa caacgaggtg cgtcgtcgca tcaccatcct
    86341 gccgcaggaa gtcgaatccc tggcgcagca ggtgggtaac caaaacgacg ccagcactga
    86401 gctgaacctg agccacatcc tgatcccgct gccggaaaac ccgacctctg atcagttgaa
    86461 cgaagcggaa agccaggcgc gcgccattgt cgatcaggcg cgtaacggcg ctgatttcgg
    86521 taagctggcg attgctcatt ctgccgacca gcaggcgctg aacggcggcc agatggggtg
    86581 gggccgtatt caggagttgc cggggatctt cgcccaggca ttaagcaccg cgaagaaagg
    86641 cgacattgtt ggcccgattc gttccggcgt tggcttccat attctgaaag ttaacgacct
    86701 gcgcggcgaa agcaaaaata tctcggtgac cgaagttcat gctcgccata ttctgctgaa
    86761 accgtcgccg atcatgactg acgaacaggc ccgcgtgaaa ctggagcaaa ttgctgctga
    86821 tatcaagagt ggtaaaacga cttttgctgc cgcagcgaaa gagttctcgc aggatccagg
    86881 ttctgctaac cagggcggtg atctcggctg ggctacacca gatattttcg atccagcctt
    86941 ccgtgacgcc ctgacccgcc tgaacaaagg tcaaatgagt gcaccggttc actcttcatt
    87001 cggctggcat ttaatcgaac tgctggatac ccgtaatgtc gataaaaccg acgctgcgca
    87061 gaaagatcgt gcataccgca tgctgatgaa ccgtaagttc tcggaagaag cagcaagctg
    87121 gatgcaggaa caacgtgcca gcgcctacgt taaaatcctg agcaactaat ggttaaaacc
    87181 caacgtgttg tgatcactcc cggcgagccc gccgggattg gcccggactt agttgtccag
    87241 cttgcacagc gtgagtggcc ggtcgaactg gttgtttgtg ccgatgccac tctccttacc
    87301 gaccgggcag cgatgctcgg tttgccgctc accctccgcc cttattcccc caactcccct
    87361 gcacaaccgc aaactacggg cacattaacg ctacttcctg tcgcgctacg tgaatctgtc
    87421 actgcggggc agttagcgat tgaaaatggg cattacgtgg tggagacgct ggcgcgagcg
    87481 tgtgatggct gtctgaacgg tgaatttgct gcgctgatca caggccccgt gcataaaggc
    87541 gtcattaacg acgcaagcat tccgtttacc ggtcataccg agtttttcga agagcgttcg
    87601 caggcgaaaa aagtggtgat gatgctggcg accgaagaac ttcgcgtggc gctggcaacg
    87661 acgcatttac cgctgcgcga tatcgcagat gctatcaccc ctgcactttt gcacgaagtg
    87721 attgctattt tgcatcacga tttgcgtaca aaatttggta ttgccgaacc gcgcattctg
    87781 gtctgcgggc tgaatccgca cgcgggcgaa ggcggtcata tgggtacgga agagatagac
    87841 accattattc cggtgctcga cgagctgcgg gcgcagggga tgaaactcaa cgggccgctg
    87901 cctgccgata ccctgtttca gccgaaatat ctcgataacg ccgacgccgt gctggcgatg
    87961 taccacgatc agggtcttcc cgtgctaaaa taccagggct tcgggcgcgg tgtgaacatt
    88021 acgctgggcc tgccctttat tcgcacatca gtggaccacg gcaccgcgct tgaactggcg
    88081 ggacgtggcg aagccgatgt cggcagtttt attacggcgc ttaatctcgc catcaaaatg
    88141 attgttaaca cccaatgaat aatcgagtcc accagggcca cttagcccgt aaacgcttcg
    88201 ggcaaaactt tctcaacgat cacttcgtga tcgacagcat tgtttctgcc attaacccgc
    88261 aaaagggcca ggcaatggtc gaaatcggcc ccggtctggc ggcattgacc gagccggtcg
    88321 gcgaacgtct ggaccagctg acggtcatcg aacttgaccg cgatctggcg gcacgtctgc
    88381 aaacgcatcc attcttaggc ccgaaactga cgatttatca gcaggatgcg atgaccttta
    88441 actttggcga actggccgag aaaatgggtc agccgctgcg tgttttcggc aacctgcctt
    88501 ataacatctc cacgccgttg atgttccatc tgtttagcta tactgatgcc attgccgaca
    88561 tgcactttat gttgcaaaaa gaggtggtga atcgtctggt tgcaggaccg aacagcaaag
    88621 cgtatggtcg attaagcgtc atggcgcaat actattgcaa cgtgatcccg gtactggaag
    88681 ttccgccgtc agcctttaca ccaccaccca aagtggattc cgccgtcgtg cgcctagtcc
    88741 ctcatgcaac gatgcctcac ccggttaaag atgttcgcgt gttgagccgc atcaccaccg
    88801 aagcctttaa ccagcgtcgt aaaaccattc gtaacagcct cggcaacctg tttagcgtcg
    88861 aggtgttaac cggaatgggg atcgacccgg cgatgcgagc ggaaaatatc tctgtcgcgc
    88921 aatattgcca gatggcgaac tatctggcgg agaacgcgcc tttgcaggag agttaacgat
    88981 gatcaattcg ccccgagtgt gtattcaggt tcaaagcgtc tacattgagg cacaatcttc
    89041 acctgataat gaacgttacg tttttgctta taccgtaacc atacgcaatc tggggcgagc
    89101 gccagtgcag ttgttggggc gttactggct gatcaccaat ggcaatggcc gtgaaaccga
    89161 agtccatggc gaaggagtgg ttggcgtcca gccacttatc gcgcctggcg aagagtacca
    89221 gtacaccagc ggtgcaatca ttgaaacccc gctgggcaca atgcagggtc actacgaaat
    89281 gatcgatgaa aatggcgtcc ctttcagcat cgacattccc gtattccgac tcgccgttcc
    89341 cacactcatt cattaaaaga atatggcgac ataccttatt ggcgacgttc atggttgtta
    89401 cgatgaactg atcgcattgc tgcataaagt agaatttacc cccgggaaag ataccctctg
    89461 gctgacgggc gatctggtcg cgcgcggccc gggttcgctg gatgttctgc gctatgtgaa
    89521 atccttaggc gacagcgtac gtctggtgct gggcaatcac gatctgcatc tgctggcggt
    89581 atttgccggg atcagccgca ataaaccgaa agatcgcctg acaccgctgc tggaagcgcc
    89641 ggatgccgac gagctgctta actggctgcg tcgccagcct ctgctgcaaa tcgacgaaga
    89701 gaaaaagctg gtgatggccc acgcagggat cacgccgcag tgggatctgc agaccgccaa
    89761 agagtgcgcg cgcgatgtag aagcggtgct gtcgagtgac tcctatccct tctttcttga
    89821 cgctatgtac ggcgatatgc caaataactg gtcaccggaa ttgcgggggc tgggaagact
    89881 gcgttttatc accaacgcct ttacccgtat gcgtttttgc ttcccgaacg gtcaactgga
    89941 tatgtacagc aaagaatcgc cggaagaagc ccctgcccca ctgaaaccgt ggtttgcgat
    90001 tcctggccct gtcgctgaag aatacagcat cgcctttggt cactgggcat cgctggaagg
    90061 caaaggtacg ccggaaggta tttacgcgct ggataccggc tgctgctggg gtgggtcatt
    90121 aacctgcctg cgctgggaag ataaacagta ttttgtccag ccgtcgaacc ggcataagga
    90181 tttgggcgaa gcggcggcgt cttaaacaca gcctgataca ggaaggccgg ataagacgcg
    90241 accggcgtcg catccggcac tagccgtaaa ttctatacaa acttaccgcc gctccagaat
    90301 ctcaaagcaa tagctgtgag agttctgcgc atcggcgtcg tggaattcgc tgaataccga
    90361 ttcccagtca tccggctcgt aatccgggaa atgggtgtcg ccttccactt ctgcgtcgat
    90421 atgcgtcaga tacagttttt gcgcttttgg caggaactgc tcataaacgc gaccgccgcc
    90481 aatcaccatg atttctggta cgtcaccaca cgccgcaatg gcttcatcca ccgatttcac
    90541 ccacgttacg cgatcgtccg tacccggttg actgctgagg ataatatttt tgcgtcctgg
    90601 caacggacga ccgattgatt cccaagtatg gcggcccata atcacgggtt tatttaaggt
    90661 gttgcgttta aaccaggcga gatcggcagg caggttccac ggcatggcgt tttccatgcc
    90721 gataacgcga tctaccgcta acgccgcaat cagactgatc attgagattt cccgataaaa
    90781 aaattgtcgc cactatacgt aaagcgtaaa ccgtcgtcga ctggtgcgag gatgatgttg
    90841 aggaaaattt tatattctgc tggcgagtcc acgctctccc cctggactcg ccgcattaca
    90901 atgaaacaaa aacaaacagt tagctgtaaa gtgtgattta cgtcactctt tattaagatg
    90961 agggtttcgt ttccggttca tccgccatgt tgccggtatg tttaccttct tctgttccct
    91021 gccagccatg tcgttgaatt aatgacagat gttcgcggtc ctcggtgatg atctcactta
    91081 acatcgcgct ggtgcgttta tagaccgccg cgcgggcttt ggtgtcattc tcaaccattg
    91141 ccatctcttc caccatctga atattaaagc ggcggaacac atcggcacgt tctcgcgctt
    91201 catacggccc cagacctaaa ctttccagcg ccagacgccc ggttttcagc gcaccttcaa
    91261 aggtttcacg ctccggcttt tcaaggcctg cctgacgcaa acgaatgtag tgatcgacat
    91321 cgcgggcgcg ggcaataatc tgcaaatgcg ggaaatgttc tttcaccatc tccgtcagtt
    91381 gcaggttggt ttgcggatcg tcaatagcgt taatcagcac ttccgctttc gccgctccgg
    91441 cagattccag taaatccatc cgcgtggcat cgccataaaa cactttcata ccaaatttac
    91501 gcagcgtttc gatatggtcc ggatcgtgat cgagcaccac cattttcacc ccgctggaga
    91561 gcagtaaacg tccggtaatc tgtccaaaac gaccgaatcc ggcgataatc acgcgcggct
    91621 gttcttcatc gatctcatcg gcttcacgcg cctcctccgt agaagattgc tcaaggcgat
    91681 tgaggatcac cagcagaatc ggcgttgctg ccatcgacag cgccaccgcc agggtcagcg
    91741 atttcgccca ctccggctcc agcacattcg ccatctgcgc cgcaccaaat accacaaagg
    91801 caaactcact gccctgccct aacaacaccg caaaccagcg acgctgttta tttggcacct
    91861 gcaacggtcg ggcaatcagc cacagcatgg cgattttgat gatgaggaaa ccgagcagca
    91921 aaatgacaat gcgcaatggg ttttcaatca gcgtgccaaa gtctatcgac atgccaacac
    91981 cgatgaaaaa cagccccaac agcaaacctt taaacggttc gatatcgctt tccaacgcat
    92041 gacggtattc cgagcttgcc agcagtacgc ccgccagaaa cgcgcccatc gccatcgaca
    92101 agccgacctc ttccagcagc aaaccaaagc caaacacaag gaataacgcc acgacactaa
    92161 acacttcccg caagccagag cgggcaacaa aacgcagcgc cggacgcgta acatagcgcc
    92221 ccagcaatac caccagtacc agcgcacccg ccacttttaa cgccgagaga gcaaatgcgc
    92281 ccatcgtcgt cgaggcactg ctcgccgcca gtagcgggat catcgccacc agcgggattg
    92341 ccgcgatatc ctggaacagc agcaccgcaa aggcactgcg gcccatttgc gtcaccatca
    92401 gattgcgttc attcatcgcc tgcatggcaa tcgccgttga tgagagcgcc agcgtcatac
    92461 cgatcaattc cgcgacctgc cagcgcagcc caagtaacat acagaacagc cccagcaggc
    92521 caccgcaaat caccatctgc aatgcgccac cgccgaacac tgccgcacgc agcttccaca
    92581 gcctttgtgg atcgagttcg aggcgataat aaacagcatc agcaccaccc caatctcggc
    92641 aaagtgcaga atagattcgg catcggtcac cagtcgcagc ccccacgggc caataatgca
    92701 gccggcaatc aggtagccaa gtaccgatcc cagaccaaga cgtaccgcaa tgggtacaat
    92761 cagcgccgcc gatccgaggt agatcagcgc ctgaaccagc gtatgactat ccatgatggg
    92821 cctcctgcca ttccagcaga cgttgcttat agtggcgcgc ctgcccttcg agggtttcgt
    92881 cgtcacaaat aaaggtgcag tgcatggcaa acggtggcag ccagttcagc ccgcagtaga
    92941 ttgccgtcgc ctgtagcggc tgcgacagca catcaaagcc cggatgcgca ccaatttcaa
    93001 aatggctttc cccgccgccg gtcgtcaccg cccacagcaa atgtttgcca tgcagcgccg
    93061 tgccgccgtg accgtaagcc cagccgtgcg acaaaacttt atcgatccaa agtttaagaa
    93121 gcggaggaat gctgtaccac tgcatcgggt gctgccagac gatcagatcg gcgcgagaga
    93181 gcgcctcctg ctcggcggca atatcgatat tgaagtcagg atagagttga taaagagagc
    93241 gaatttcgac gccttccagc gtccttgcct gttcaagcat ccgtttattc gcatgggaat
    93301 gatgcggata cggatgcgca taaatgatga ggatcatagt tattagcccg cgtgtttacg
    93361 cctttgtttt cccacaaagt gtagtcatat attcacgcgg cttatagtca ataatattga
    93421 ttcagaaaag agagaaatct gaacaaataa tcccattctc tgcccgcgga tcgtcatatt
    93481 gctggtctgc gccagtgaca ttcctcgagt ctccggggca aacgctacgg aaatcaacaa
    93541 gccaaacagc gagatacccg cccccattag catcgtgtta ctgataccgt aattattgat
    93601 aaatatcggc agtgcccagg tcgaaacaat ggtgccaata cgacttaagg acataatcac
    93661 gcccacggca gaggcgcgga tatccgtcgg gaagagttca ttaggataga gccactgcaa
    93721 attacccggc ccgccagaga aaaaggcata caccgcaaag gccatcacta ccagccagat
    93781 ccccatatcc gggattagcc ccaaaaccgc cagcgccagc gtcatcatgg caaagctgcc
    93841 aatcaacaat ggacgccgtc cggcagtgtt tagccacagc atcggcggaa tacagccgag
    93901 cataaagaac aggctaatca ccacattccc tagtgccgcg tttttgccaa cccccaatcc
    93961 caacgaacca acgatttgcg ggccaaaggt gtaaatggcg aacattggga tcacctggca
    94021 ggtccagatg gcggcaacaa acagaacaaa aggaaaatgg cggcgattaa acagatcgcg
    94081 aaaacgggtt tgctgcggct gctcttcatc gaaagccacc ggttcgccaa acagttttat
    94141 catcatctct tcgcactctt ttactcgccc tttgcgtaat aaccagcggg gagattctgg
    94201 cagttcgaat cgaccaatca aaatcaacaa acaagggatc gccgcgctac ccagcatcca
    94261 gcgccaacca ccttccacat cataaagcca gtagccgacc agatcggcgc aggtcgcgcc
    94321 gacataccac atcgcggcaa taaagctgat ggaaaacgcc cgctgacggg tactggagaa
    94381 ctcggtgatc atcgaagtgg cgatgggata atctgcaccg atgacaatgc cgatgagtac
    94441 ccgcatcacc aacagttcga cgggggatga aacacacatc gtcgccaccg atatcacgcc
    94501 gatggcgatg atatcaatga ggaacatttt gcgccgtccg actttatcgg agatatagcc
    94561 aaacagcgat gtgccaacga acagcccggc gagcgttccc gcgcccagca agccaatcca
    94621 gtcagcgtcc agtttcagcg ccggagtcag ttgctccagc gccacgccaa tcattaccag
    94681 tacataacca tccagaaacg gaccaccgct tccccacagc aaaatgcggc ggtgaataga
    94741 ggagaatttg agatcgtcaa agtttctgga cggttgcatg ggttattcct gtttttttgc
    94801 aatgagagtc agtacggcgt tacgtatcaa accaacatca gccgtaacgg aactccacac
    94861 caaaggtgcc gcgcgggtat tcccactgtt ccagcgccga ccccagcccc aaaatgcgac
    94921 aggtgccgca ctccagacat ccggcgtaat cgaagcgcac actgccgtca tcctgcttct
    94981 tgtacagacc tgcggggcac gctttcacca gcagctccag cacctgttta tcagcatcgg
    95041 ccttcacaac aatgtgcgga tgctcttcat cgacattgaa tttattgacg cccagtttga
    95101 cgtccacatt gacgggagaa gtcataaaac ggtcactcct ttcatgccat ccttgatcag
    95161 attgatgaag cccactttat tgccgtggcg gaggattttc ttgcgcatca gttccggcgc
    95221 actgccgtca atggtgaaca ggtcacgcgc cacgcccacc gccagttccg ggtagccgct
    95281 aaacatgcgt gggttatcaa ggaacgccgg tagtttctgg tacatgcgca tatcgcgcag
    95341 cgggccactc tccagatgct gacgatattc cgcgagtttt tgcttactga aatcgtcgct
    95401 tttcatcgct gaaagcacgg tttttgctgc agcttccccg gcggcaatcg ccagatccat
    95461 accgcgaatg gtaaaaccga ggttcataca cattccggcg gcatcaccgg caatcaatac
    95521 gccgtcaccg accagctccg gcagcatgtt gatgcctgct tccggcacta cgtgagcgga
    95581 atattccacc agcttgccgc ccgcgatcag cggtgcaacg gccggatgct gtttgaaatc
    95641 ttccagcatt tgcggcaccg attttttcgc gtcatgcaga tgatgcagac cacaaaccag
    95701 ccccagcgac agggtatttt cactggtata aaggaagccg ccgcccatca ggccatcggt
    95761 gggtgatccc gcaaacagac aagccgcgcc ctgattaccc tgcaactgaa aacggtcttc
    95821 gattaccgac ttcggtaact cgatcagttc cttcacgcca accgccacat ccgtcggttt
    95881 aacgcgtttt gccatcccca acttttcggc aaggatggag ttcaccccat cagcaaggat
    95941 caccgttttc gcttcaatca catcgccatc ggcttctaca ccgacgactt tgccatcgcg
    96001 ctgtacgagg ttatcgacgc ggatcccggt aattaactgc gcgctcgctt cttcggcctg
    96061 ctccatcaac caggcatcaa atttactgcg caaaacggag taagaacgct gggatggcga
    96121 ggtttcgtca ccgttgcagt agtccatagt catcgctgac ttttccgtca taaacgcgag
    96181 tttttcatgg gtgatcaggc gttctacggg ggcggaatcg gcgaaacctg gaataatgtg
    96241 ttccagactg tgggcataga gacgcccgcc agtaacgttc ttggcacctg cggaattgcc
    96301 acgctcgata actaacactt gcgccccttc gcgggcgagc accagtgcgg caaccgaacc
    96361 ggcaagccct gcaccgacga tgatggcgtc aaagatatct tcggacataa gagctccctg
    96421 tcagcggcag agccgtaatg cagccctgcc agagtggatc aacacgctaa agctgcggtc
    96481 agtgccggaa ggatcttcac ggcatcgcca acaatgccgt aatccgcgta ctggaagatc
    96541 ggcgcatttt tatctttatt gatggcgaaa atggtttgcg acgcgttagc gccaaccatg
    96601 tgctggatct gcccggagat ccccaccgcc aggtacagtt caggtttcag catcaggttg
    96661 gagataccga catagcgttc gtgctccatc catttttcgt tttctgccac cggacgagaa
    96721 caggccaact ccgcacctat cgccttgcaa agctgttctg ccagcgcaat gttctctttg
    96781 ctgccaatgc cgcgaccgac gctgaccacc agacgggctt tgtcgagatc gacgctgttg
    96841 ctctggcgcg cctgggttgc cgtgcgggta atcgccacag ccggagcctg ccactccacg
    96901 gtgtgcgttt cgccagtgcg tgacgcgtct ggctgagccg catcgaacgt gccgctgctg
    96961 atggtcagta ccgcatacgg cgtggcaatg cgttcttcgc caatcgccag accgccgtaa
    97021 accatgtgtt tcactgttgc tttaccgtcc tgtacgctga cggtgctggc atcgttagac
    97081 accgccgctt taaggcgata acccagtttt gccgccagta atttgccgcg acgggtgttt
    97141 ggcaacagca ccagaccgtc agcgccgtgc tggcgaatag tgtcagccat gacaccggcg
    97201 taatcttcga tcatccgatc gtccggtttg ccgtttaatt tccagacatg attagcgccg
    97261 agctggattg cctgtgcgcc gtcggcatca ttgaggacaa aggcattgat ttgattagct
    97321 aaagcctgcg caccgttcat cagttccggc agacgagagg gggtatcgct gaatacccag
    97381 acttgagaaa acgtgttcat agcatcccct gtaattaaat gactttgcga agattttcag
    97441 caaatgcggc gatctgttct tcgccgtcgc cttcaatcac gatgcgctga cgctcgcgct
    97501 gtttcggcgc ggcaacctgt tgttcagacc aggcgtccac ggcgttaaaa ccaatatccg
    97561 ccgccgacca tacctggact ggctttttcg ccgcgccgag aatggctttc atcgaaggaa
    97621 tttgtgggga gttgatatca gtggaaacag caacaaccgc aggcagcgga atgcttaagg
    97681 tttcggtttc atcttccagt tcgcgctcaa cggtgagggt atctgccgtc agggagataa
    97741 ttttgctgac gccgttaact gccggaatat tgaggatttc gcccaccagc agaccaacct
    97801 gctgggcata aaggtcggaa gaaccatcgc cacagaggat cagatcaaag cctgctttct
    97861 gggcggctgc agccagtgcg ctcgccgttt gttgcggcag tgcctgctcg aactggtcat
    97921 caatcaccac aatcagttca tccgggccgc gcgatagcac atctttacgc cctttggcgt
    97981 tggtcagggc tttaccgccc acacttaagg ctgtcacctg cgcctctgct gcctgttgct
    98041 ttagctggca agccgcttca atagcgttga gatcgtattg gcttattttg gcatcggctt
    98101 tgctgaagtc taatgaacca tcagcattat tgaccgcaat atcctgttca tcaggcacgc
    98161 acttatagca agtaataatc ttcattgcat ctccagaaat catgaaggaa acgtcgttca
    98221 tatcggtggt gttaattaca gatatcacgc cctttaattt ggaaataaaa ctttaatcac
    98281 caatattgaa aatgtcacac gcagataaat ccaactttca atattgtttt atagatcacc
    98341 aatattgaaa gtcagatgct atttttttac ccacaaaaga acataacaaa ctgattaata
    98401 accaaaatta acggaaaacg tcctctattc acccgaaaca aaaatgtgac aacaatcaca
    98461 gaatacagct tattgaatac ccattatgag ttagccatta acgcgtccac gaggttaata
    98521 ataattatat taaatgttaa caaaaataaa acaaacggga acgcaaaata aatattcgtt
    98581 ttcacagtgg aattaactca tgaagaatga aaagagaaaa acgggaatag aaccgaaggt
    98641 tttctttccg ccattaataa tcgtcggcat actttgttgg cttacagtca gagatctgga
    98701 tgcggcgaat gtcgttatta atgctgtatt cagttacgtc accaatgtat ggggatgggc
    98761 atttgaatgg tatatggtgg tgatgctttt cggttggttc tggctggtgt ttggcccgta
    98821 tgccaaaaag cgtttaggta acgaaccgcc agaatttagc accgccagtt ggatctttat
    98881 gatgttcgcc tcctgtacgt ctgctgccgt actgttctgg ggatcgattg agatctacta
    98941 ctacatctcc accccgccgt ttggcttaga accgaactcg acaggagcga aagagttggg
    99001 gctggcttac agcttgttcc actggggacc tctgccgtgg gccacttaca gcttcctttc
    99061 agtcgccttc gcttacttct tctttgtccg caaaatggaa gtgattcgcc ccagctcgac
    99121 actggtgccg ctggtaggtg aaaaacacgc caaagggttg ttcggtacca tcgtcgacaa
    99181 cttctatctc gtcgccttga tcttcgcgat gggtaccagt ctgggccttg ccacgccgct
    99241 ggtgaccgag tgtatgcaat ggttgtttgg cattccgcat accctgcaac tggacgctat
    99301 catcattacc tgctggatta tcctcaacgc catttgcgtc gcttgcggtc tgcaaaaagg
    99361 ggtacgtatc gccagtgacg tgcgtagtta cctgagcttc ctgatgctgg gttggatgtt
    99421 catcgtcagc ggtgccagct tcatcatgaa ctacttcacc gattcggtgg ggatgttgct
    99481 gatgtatctg ccgcgcatgt tgttctatac cgatcccatc gctaaaggcg gcttcccgca
    99541 gggctggacc gtgttctact gggcatggtg ggtgatttac gccatccaga tgagtatctt
    99601 cctcgcccgc atctcccgtg gtcgtactgt gcgtgaactg tgcttcggca tggtgatggg
    99661 gctgacagca tccacctgga tcctgtggac cgtgctcggt agtaacactc tgctgttgat
    99721 ggataaaaac atcatcaaca ttccaaatct gatcgaacag tacggtgtgg cgcgcgccat
    99781 catcgaaacc tgggccgctc tgccactcag caccgccacc atgtggggct tcttcatcct
    99841 ctgctttatt gccaccgtta cgctggttaa cgcctgctct tataccctgg cgatgtctac
    99901 ttgccgcgaa gtacgcgatg gtgaagaacc accgctgctg gtgcgtatcg gttggtcaat
    99961 tctggttggc attatcggta ttgttctgct ggcgctcggc ggcctgaaac cgattcaaac
   100021 cgccattatc gccggaggat gcccgctgtt cttcgtcaac attatggtga cgctctcctt
   100081 tattaaagac gcgaaacaga actggaaaga ttaattaacc cccaaaatat caagaggttg
   100141 aaagatggat tttaatttaa atgatgagca ggaactgttt gtcgccggta tccgcgaact
   100201 gatggccagc gaaaactggg aggcctattt tgccgagtgc gaccgtgaca gcgtctaccc
   100261 ggaacgtttt gtcaaagcac tggcggatat gggtatcgac agtctgctga tccctgaaga
   100321 gcacggtggt ctggacgcgg ggtttgttac tctcgccgcc gtgtggatgg agctgggacg
   100381 tctgggggca ccaacctatg tactgtacca gctgccgggc gggttcaata ccttcctgcg
   100441 cgaaggcaca caagagcaga tcgacaagat tatggctttc cgcggcaccg gtaagcagat
   100501 gtggaactca gcgattaccg aaccgggcgc gggctccgac gtgggtagcc tgaaaacgac
   100561 ttatacccgt agaaatggta agatttatct taatggtagt aagtgtttta ttaccagtag
   100621 cgcctacacc ccgtacatcg tggtgatggc gcgcgacggg gcttctccgg acaaacctgt
   100681 ctacaccgaa tggtttgttg atatgagcaa accgggcatc aaagtgacca aacttgagaa
   100741 gctcggtctg cgtatggata gctgctgtga aatcaccttt gacgatgtgg aactggacga
   100801 gaaagacatg ttcggtcggg aaggtaacgg ctttaaccgc gtcaaagaag agttcgacca
   100861 tgaacgtttc ctggtagccc tgaccaacta cggtacggcg atgtgcgcct ttgaagatgc
   100921 tgcgcgctac gccaaccagc gcgtgcagtt tggcgaggct attggtcgtt tccagttgat
   100981 tcaggaaaaa ttcgcccaca tggcgatcaa attaaactcc atgaaaaaca tgctgtatga
   101041 agcagcgtgg aaagcagaca acggcaccat cacctctggc gatgcagcga tgtgcaaata
   101101 cttctgcgcc aatgcggcat ttgaagtggt ggatagcgca atgcaggtgc tgggcggtgt
   101161 cgggattgcg ggcaaccacc gcatcagccg cttctggcgt gacctgcgtg tagaccgcgt
   101221 ttccggagga tctgacgaaa tgcagatcct gacgctgggt cgtgcggtgc tgaagcaata
   101281 ccgctaagtc cgtgcgctgc ctgatgcgac gctgacgcgt cttatcaggc ctacgggagc
   101341 acgaatgtag gccggataag gcgtttacgc cgcatccggc agtcatgcgc tacataaatt
   101401 tttcaggagc taataatgga tcatctaccc atgccgaaat tcggaccgct ggccggattg
   101461 cgcgttgtct tctccggtat cgaaatcgcc ggaccgtttg ccggacaaat gttcgcagaa
   101521 tggggcgcgg aagttatttg gatcgagaac gtcgcctagg ccgacaccat tcgcgttcaa
   101581 ccgaactacc cgcagctctc ccgccgcaat ttgcacgcgc tgtcgttaaa tattttcaaa
   101641 gatgaaggcc gcgaagcgtt tctgaaatta atggaaacca ccgatatctt catcgaagcc
   101701 agtaaaggtc cggcctttgc ccgtcgtagc attaccgatg aagtactgtg gcagcacaac
   101761 ccgaaactgg ttatcgctca cctgtccggt tttggtcagt acggcaccga ggagtacacc
   101821 aatcttccgg cctataacac catcgcccag gccttcagtg gttacctgat tcagaacggt
   101881 gatgttgacc agccaatgcc tgccttcccg tataccgccg attacttttc tggcctgacc
   101941 gccaccacgg cggcgctggc agcactgcat aaagtgcgtg aaaccggtaa aggcgaaagt
   102001 atcgacatcg ccatgtatga agtgatgctg cgtatgggcc agtacttcat gatggattac
   102061 ttcaacggcg gcgaaatgtg cccgcgcatg agcaaaggta aagatcccta ctacgccggt
   102121 tgcggtctgt ataaatgtgc cgacggctac atcgtgatgg aactggtggg cattacccaa
   102181 attgaagagt gctttaaaga tattggcctc gcacatctac tcggtacgcc ggaaatcccg
   102241 gaaggcactc agcttatcca ccgtatcgaa tgcccttacg gcccactggt agaagaaaaa
   102301 ctcgatgcct ggctggcggc acataccatc gcagaagtta aagaacgctt tgccgaactg
   102361 aatatcgcct gtgccaaagt actgaccgta ccggaactgg aaagcaatcc acagtatgtt
   102421 gcccgtgaat caatcactca gtggcaaacg atggatggtc gcacctgcaa agggccgaac
   102481 atcatgccga aattcaaaaa taaccccgga caaatctggc gcggaatgcc ctcacatggc
   102541 atggacacgg ctgccatttt gaaaaatatc ggctacagcg aaaacgacat tcaggagttg
   102601 gtcagcaaag gtctggccaa agttgaggac taaacgcgtg gcgtcagtcg gcttgcctga
   102661 ctagcgcccg cccaataaca aacgaacaaa aatggataga ggtgcaatgg atatcattgg
   102721 cggacaacat ctacgtcaaa tgtgggacga tctggcggac gtttacggtc ataaaacggc
   102781 gctgatttgt gaatccagcg gcggagtcgt taaccggtat agttatcttg agttaaatca
   102841 ggagattaac cgcacggcaa acctgtttta tacgctgggg attcgcaaag gcaacaaggt
   102901 tgcactacat ctcgacaact gcccggaatt tatcttttgc tggttcgggc tggcaaaaat
   102961 tggcgcgatt atggtgccga ttaacgcccg cctgttacgc gaggaaagcg agtggatctt
   103021 gcaaaatagc caggcgtgcc tgctggtgac cagtgcgcaa ttttatccta tgtatcaaca
   103081 gattcagcag gaagatgcca ctcaattgcg gcacatttgc ctgacagatg tggcacttcc
   103141 cgctgatgat ggagtgagtt cgtttactca actgaaaaat caacaacctg ccaccttgtg
   103201 ctatgcaccg ccgctatcga ctgacgatac ggcggaaatt ctcttcacct ccggcaccac
   103261 ctcccgaccg aaaggtgtgg tgattaccca ttacaacctg cgcttcgctg gatattactc
   103321 cgcctggcag tgtgcactgc gtgacgatga cgtctacatg acggtaatgc ctgcgtttca
   103381 tatcgattgc cagtgtactg cggcgatggc ggcgttttct gccggggcca cctttgtgct
   103441 ggtcgagaaa tacagcgccc gcgccttctg gggacaggcg cagaagtacc gcgccaccat
   103501 taccgaatgt attccgatga tgatccgtac gttgatggtg cagccgcctt cagcgaacga
   103561 tcggcaacac cgcctgcggg aagtgatgtt ttatctcaac ttgtcggagc aggaaaaaga
   103621 cgcattttgt gaacgcttcg gcgttcgctt gctgacgtct tatgggatga cggaaaccat
   103681 tgtgggcatt atcggcgatc accctagcga taaacgacgc tggccgtcga ttggtcgggc
   103741 ggggttttgc tacgaagcgg agatccgcga cgatcacaat cgcccgctcc cggcaggtga
   103801 gatcggtgaa atctgtatta aaggcgtacc agggaaaacc atcttcaaag agtattttct
   103861 caacccgaaa gccactgcga aagtgctgga agccgacggc tggctacata ccggcgatac
   103921 cggataccgc gacgaagaag gcttttttta tttcgtcgat cgccgctgca atatgatcaa
   103981 acgcggtggc gagaatgtct cctgcgtgga gctggaaaat atcatcgcca cccatccgaa
   104041 aattcaggac atcgtggttg tgggtattaa agattcgatt cgcgatgaag ccatcaaagc
   104101 atttgtggtg ctgaatgaag gtgaaacatt gagcgaagag gaattttttc gcttctgcga
   104161 acaaaatatg gcgaaattta aagtgccctc ttatctggag atcagaaaag atctgccacg
   104221 taattgctcg gggaaaataa ttagaaagaa tctgaaataa atgaaacggc agggaactac
   104281 cctgccagct aataatcacg ccctaaaaca atatgcattt tttgcaggga tgttgtcgtc
   104341 cctaaaaaag caaaaatgga gaaaaggaat gagtgaatca ttacatctga cccgcaatgg
   104401 atcaattctg gaaattaccc ttgatcgccc aaaagcgaat gctattgatg caaaaaccag
   104461 ctttgaaatg ggcgaagtat ttctaaattt ccgtgacgat ccgcaattac gtgtcgccat
   104521 tattaccggt gccggagaga aattcttttc cgcgggctgg gatttaaaag cggcagcaga
   104581 aggcgaagca ccggatgctg actttggccc gggtggtttt gcgggattaa ccgaaatttt
   104641 caatctcgac aaaccagtta tcgcagctgt gaacggctac gcctttggcg gcggtttcga
   104701 actggcgctg gcggcagatt ttattgtttg tgccgataac gccagcttcg ccctgccgga
   104761 agccaaactg ggcatcgttc ctgacagcgg cggtgtgctg cgtctgccga agatcctgcc
   104821 gcctaccatc gtcaatgaaa tggtgatgac cggcagacga atgggcgcag aagaggcgct
   104881 gcgttggggg atagtcaacc gcgtggttag ccaggcggaa ctgatggata acgcccgcga
   104941 actggctcag cagctggtta acagcgcccc gctggcgatt gcggcgctga aagagatcta
   105001 ccgcaccacc agcgaaatgc cggtagaaga agcgtatcgc tatattcgca gcggcgtgtt
   105061 gaaacactat ccatcggttc tgcattcgga agatgccatt gaagggccgc tggcgtttgc
   105121 cgagaagcgc gatccggtgt ggaaaggacg ttaacaaccg tgagctatta cgcctttgag
   105181 gggttaattc cggtggttca cccgacggcg tttgtccatc ccagtgccgt cttgattggc
   105241 gatgtgattg tgggagccgg tgtctacatc ggcccactcg cctcactgcg tggtgactac
   105301 gggcggttga tcgtgcaagc gggagccaat attcaggatg gctgcattat gcatggctac
   105361 tgcgacactg acaccatcgt tggggaaaac ggccatatcg ggcatggagc gatcctgcat
   105421 ggttgtgtga ttggtcgcga tgcattggtc gggatgaaca gcgtaattat ggatggcgcg
   105481 gtcattggcg aagagagcat tgttgccgca atgagctttg tcaaagcagg ctttagtggc
   105541 gagaaacgcc agttgttgat gggtacgcca gcccgcgctg tacgcagtgt tagtgacgac
   105601 gagttacact ggaaacggtt gaataccaaa gagtatcagg atcttgtcgg gcgctgtcat
   105661 gcatcgttac atgaaacgca gccactgagg caaatggagg aaaatcgccc ccgtttgcag
   105721 gggacgacgg atgtgacgcc taaacggtga tcttgccgga tgcgacctgg ttgctggata
   105781 agatgctaca acatcgtatc tggcaagcag gacgcccggt atcaacgaga taaaattaac
   105841 gacgcatact ctttgacaac atcatctgcc acttctgctc gcggttaagt tccgtgagcg
   105901 gaacggcagg aatacgcggt gtctttctta cgccgactaa tttttcccga ctgttattgc
   105961 gcaggcgcgc gtaaatttgc tcatcgatat cgaccacttt aaccagtcgc tgacactggc
   106021 atccccgccc ttccagctta ttagggatca tcttaacttc gcagcttatt tctgtgactt
   106081 ccgacagaat ataagtcagg gtattaaccg ccttgctgtg ttcaatatcg aaatgaatag
   106141 agatctctct tgctatcacc caccgatttt cagccatcat ccattcggcg attaacaagt
   106201 agagtagttt ttcaacatat ccttcacaca tacgaactcc agtttcacaa attaaaagtc
   106261 tggctatgac tcgttttgaa gcaattaaac aaggccatat taaaattgtg gatatatcga
   106321 tagtatgcaa cttcaccgtc gataagtgtg agctaaatcc tgcttatgtc atcaaaaata
   106381 tcgattcccc aaaagattta cttaatgggc aaaaaaaaac ggtcctcatc agagaaccgt
   106441 attagttatt cgatcaaatt agcggatgaa aaatatccgc cacgacacgc tattatttga
   106501 tctgtgcgtg catttcctgc accgaaatta ctttttcagt cgcatcggca ttcagcgcca
   106561 tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg cactttatat tgcagcgcac
   106621 tgcgacggat cacgcgggag tcttcaatcg cacgacggcc tgaggtggtg ttgatgatgt
   106681 aggtatattc gccattcttg atacggtcct gaatgtgcgg acggccttca tgcaccttgt
   106741 ttaccagacg cggattgata cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat
   106801 ccagctcgaa gccctgtttc agcagttttg ccgccaggtc caccacgcgt tctttatcgc
   106861 cttcgcgcac ggaaagcagc gcacgaccgt gtttcttcat ggtggagttg ctgcccagct
   106921 gcgctttggc gaacgcttca gcgaaggtgc ggcccacgcc catgacttcc ccggtagagc
   106981 gcatttctgg ccctaacagc gggtcaacgc ccgggaattt attgaacggc agcaccactt
   107041 ctttcaccga gtagtacggc ggaataactt ctttggttac gccctgctca gccagcgatt
   107101 tgccagccat cacacgcgcc gccacttttg ccagcggtac gccggtggct ttggagacga
   107161 acggaacagt acgcgccgca cgcgggttta cttcaatcag gtaaacttcg ttgtttttca
   107221 ccgcaaactg cacgttcatc agaccgcgca cctgcaattc gaaagccagt ttctgcacct
   107281 gctggcgcat cacatcctga atttcctgac ttaaggtgta cgctggcagc gaacatgcgg
   107341 agtcaccgga gtgcacgccc gcctgctcga tgtgctccat gatgccgcca atcagcacca
   107401 tttcgccgtc gcagatggca tccacgtcaa cttctactgc gtcatcaagg aaatgatcca
   107461 gcaacactgg cgcatcgtta gacacgctaa ccgccgtctg gaagtagcga cgcaggtcag
   107521 cttcgtcata gacgatttcc atcgcccgac cgccgagaac gtaagacgga cgcaccacca
   107581 gcgggtagcc aatctctttt gccttctcaa ctgccatttc aatagcagta acggtggcgt
   107641 tcgccggttg tttcagtttc agacggtcaa ccgcatgctg gaagcgttca cggtcttccg
   107701 cacggtcgat agcatccggg ctggtgccga taaccggtac gccagcggct tccagtgcac
   107761 gcgccagttt cagcggggtc tgaccgccgt actgaacgat aacgcctttc ggcttctcaa
   107821 tacgcacgat ttccagcaca tcttccagag ttaccggctc gaagtagagg cgatcggaag
   107881 tgtcgtagtc ggtagagacg gtttccgggt tacagttaac cataatggtt tcgtaaccgt
   107941 cttcacgcag cgccagcgag gcgtgtacgc agcagtagtc gaattcgata ccctgaccga
   108001 tacggtttgg accgccgcca agcaccatga ttttttcacg gtcggtagac ggattcgctt
   108061 cgcactcttc ttcataagtg gagtacatgt aagcggtgtc ggtggcaaac tctgccgcac
   108121 aggtatccac gcgcttgtag accgggtgca ggtcatattg gtcacgcagc ttacggattt
   108181 ccgcttcgcg tacgcccgcc agttttgcca agcgcgcatc ggcaaagcct ttgcgtttca
   108241 gctggcgcag gaattcagcg ttcaggccag tgatgcccac ttccgccact ttctcttcca
   108301 gacgcaccag ttcttcaatc tgtaccagga accagcggtc aatgttagtc aggttgaaga
   108361 cgccgtccac agacaggccc gcgcggaaag catcggcgat gtaccagata cgctctgcgc
   108421 ctgcgtcttt cagttcgcga cggattttgg ttaacgcttc cgggtcatct aggctcactt
   108481 tcgggtcgaa gccagttgcg cccacttcca ggccgcgcag cgctttttgc agggattcct
   108541 gctgcgtgcg accaatcgcc atcacttcgc caaccgattt catctgagtg gtcagacggt
   108601 cgttagcacc ggcgaatttt tcgaagttga agcgaggaat tttagtaacc acatagtcga
   108661 tggacggctc gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc agttcgtcga
   108721 gggtgtaacc caccgccagt ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg
   108781 acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc gataacaatc aggcgaccgt
   108841 ttttcgggtt caccgcaaac tggacattgg aaccaccggt ttcaacgccg atttcacgca
   108901 gcaccgccat cgaggcgtta cgcatgattt gatattcttt gtcggtcagc gtttgggctg
   108961 gcgcgacagt gatggagtca ccggtgtgga tgcccatcgc atcaaagttt tcgatagagc
   109021 agacgatgat gcagttgtcg tttttatcac gcaccacttc catctcgtac tctttccagc
   109081 cgatcagcga ctcatcaatc agcaactctt tggtcggaga gagatccaga ccgcgggcgc
   109141 aaatttcttc aaactcttca cggttataag cgataccgcc gccgctaccg cccatggtaa
   109201 aggatgggcg aataatgcac gggaagccca cgtcagcggc aacagccagc gcttcttcca
   109261 tcgtgtgtgc gataccggaa cgcgcggttt ccagaccaat tttcttcatc gctacgtcga
   109321 aacgacggcg gtcttctgct ttatcaatcg catcggcagt ggcaccaatc atggtgacac
   109381 cgaactcttc caacacgccc tgacgttcca gctccagcgc gcagttcagc gccgtctgac
   109441 cgcccatggt tggcagcacc gcgtccgggc gctctttttc aataatcttg cgtaccactt
   109501 cccagtgaat cggctcgatg taggtcgcat cggccatttc cgggtcggtc atgatggtcg
   109561 ccgggttgga gttcaccaga atgacgcggt aaccctcttc gcgcagggct ttacatgctt
   109621 gcgcgccaga gtagtcaaac tcacacgcct gaccgataac aatcgggccc gcacccagaa
   109681 tcaggatact ttttatatct gtacgttttg gcatggctct tttactcctg attacttagc
   109741 ggttttacgg tactgctcaa ttaacttgat aaagtggtcg aacaacggcg cggcgtcgtg
   109801 tggaccaggg ctggcttcag ggtgaccctg gaagctgaac gccggtttat cgatgcgatg
   109861 aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa
   109921 tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca cgtttttctc
   109981 cacatcttta accggatggt tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc
   110041 accgctcgcc agcgccagca gctgatgacc gagacagatg ccgaataccg gaatatcggt
   110101 ttcgaggaac ttctggatgg cggtaatggc gtaatcgcac ggggccgggt cgccaggacc
   110161 gttggagagg aagatgccgt ctggattcat tttcagcaca tcttccgcag aagtttgcgc
   110221 cggaacgatg gtcaggcgac agcctctatc caccagcatc cgcaggatgt tgcgcttggc
   110281 accaaaatca taagccacga cgtggaacgg cagctcgtct tcttttttcg cttctggcag
   110341 gccgccggtc aacgtccagc tcccttgtgt ccagctatag gcttctgcgg tggtcacttc
   110401 ttttgccaga tccatgccgt tcagacctag gaacgcgcgg gctttttcta acgccagcgc
   110461 cgcatccggg ttatcgcctg cgataatgca gccattctgt gcgcctttct cgcgcagtaa
   110521 acgcgtcagc ttacgggtat cgatatcggc aatcgccacg atgttatggc gcttcaggta
   110581 agaagagagg tcttcggtat tacggaagtt gctggcaatc agcggcaggt cgcgaatcac
   110641 cagaccttgt gcatgtacct gagaagattc ttcatcggcg tcattagtgc cgacattgcc
   110701 aatatgggga taagtaagag taacgatttg acgagaatag gaaggatcag tgaggatttc
   110761 ttgataaccg gtcattgaag tattgaaaac gacttcccca accgccgaac ctgttgcccc
   110821 tatggcccga ccgtgaaact gggttccgtc ttccagaacc aatagcgctg acttaatcaa
   110881 aacaccctcc agagaatatt cactcacttt atttgcatat taattcacag tgatgacgta
   110941 aatcaatgca aatctgctta ccgacgaatt tctggcaaac ggcggcattc tggagatatg
   111001 ggcgctaaaa gtcaacttaa tggcaggatt ttttatatta ttttgtggct catgcatgtt
   111061 ttacaggcat taagcgacaa aaacatcatc taacctgttc tggaaaacgc ttgcgcagca
   111121 agaagattgc atgaaataaa aacgagcagg aaaaagtgga ccaaatggtc aaaatttaca
   111181 ttaaaacaac aaaaatagaa gacgaaacat gtaatttttg gcagttatag aaaatttaaa
   111241 agcataacac caaaaataaa agggctaaaa attaaagccc tttataatta ataaattaaa
   111301 tgtgttactt ttgcatcata ataaacattt tgtggttaca aattattgag atcaagcaca
   111361 tctcgcatat caaaaagacc actttcctta ccactcaacc acaaagccga tcttaccgca
   111421 ccgttagcaa atgtcatgcg gctggacgcc ttatgggtga tctccagacg ctcgccaata
   111481 tcggcaaaca tcgcggtatg ttcgccaacg atgtcacctg cacgcacggt ggcaaaacca
   111541 atggtaccag gcacacgttc accggtatgg ccttcacgac tgtagaccgc gcaatctttc
   111601 agatctttat caagggcgtg ggcgatcgcc tctcccattg ccagtgcggt gcctgacggc
   111661 gcatcaactt tatgtctatg atgtgcttca ataatttcga tatcggtgta gtcacccatc
   111721 actttggctg ctttctccag cagcttaagc atgacgttaa cgccaacgct aaaattggcg
   111781 gcaaagacaa tcgcaatatc ggcggcggcg tcacgaattg cctgtttacc ggcttcgtca
   111841 aaccccgtag tgccgatcac catccctttg ccatgctgcc gacaaaaagc gagatggttc
   111901 agcgtacctt ccggacgggt aaaatcgata aacacatcaa aatcatcttt tatcgcatca
   111961 agactgcttt gcacggtaac gcctgttttc cctgcaccag ccagctcacc ggcgtcgctg
   112021 cccagtaaag aagatccttc gcgctccagc gccgcgccca gctgcacgcc ttctaatgcc
   112081 agtgccgcct gaatcaactg gcggcccata cgccccccgg ctcccgcgat ggcaacgcgg
   112141 atgtttgcat catgcatagc tattctcttt tgttaatttg catagaccgt tttcagagta
   112201 accagcgcat cgctacgccg ccagccaaaa acgctgataa ttagaaatta atgataaaca
   112261 gggagggata tcagtgaaag gcatgagtcg atgaatgact gcatgccgat aacatgtgac
   112321 aggttacggc accagagcca gcacctcagc cacccactgt tggaagcctt ttacattcag
   112381 atccaatgcc acctgtacat tggctggttt gcccaggcaa ccgtcgatat caaccaccgt
   112441 cgtgcctgag gtaaattcgc cttgagtttc taccgcgaca aaacacggct tgagggtgaa
   112501 taagtccggg cgcaccagcc aggcgatggc gcagagatcg tgcattcgca gaccgctttg
   112561 catactgccg ctacggtagt ggctaaacag ggcgtggagc atttttccgg tgcgattcaa
   112621 ctccggtaaa gtagcgagat agtcaggggt taatattgcc tgattggtga catccagacc
   112681 gcacatgaca atttcgatgc cgctacggaa gacacaggca gccgcttctg gatcggcagc
   112741 gatattaaac tcggcatttg gtgtacagtt gccgcgacca gcagaaccgc ccatgatcac
   112801 cagacgacga atatacggtt tacattccgg gcattgtaaa agtaacagcg caatattggt
   112861 taacgggccg atggccacca gggtaacggg ctctggtgca cgcatcaggg catcccgaat
   112921 cgccagaaac gccggtatcc cgagtggctt tcggttgtgc tcaacaaagt cgtagccagc
   112981 cattcccgat tcgccgtgca cagatgctgc atcacgcggt gcgcgtacca gcggcacagc
   113041 ggctccctgg gcgagcggaa tctccgcatt ccagaaatgc agcagttgca gggcattgcg
   113101 ggtagttttc tcaaccgaga cattacccgc gacggtggtc atcagttgca ggtcgagttc
   113161 gggggcaaaa atcgcggcgg caatggcgac ggcatcgtca atgccggggt cagtatcgag
   113221 gaagataggt aaacgcatgt tttcttcata aaaaatgccg gtaacaagac cggcattttc
   113281 gcataactta ggctgctaat gacttaatcg acttcacgaa tatcgacacg cagctctttc
   113341 ggcacttcga aaacaatatt ttcttcacgg ccttccagcg gaatggcttc accaccaccc
   113401 aactgctgca aacgtgccac cacattctgc accagaatat ccggagccga tgcgcccgca
   113461 gtcacgccga cgcatttaac ctctttcacc cactcttcct ggatatcttt cgcatcgtca
   113521 atcaaaaacg cgtgtttgcc catacgctgg gccagctccg ccagacggtt ggagttggag
   113581 gagtttttcg aaccgaccac caacacaact tccgcctgtt ctgccagggc gcgtaccgct
   113641 tcctgacggt tagtcgtggc gtagcagatg tcatctttgc gcggaccgac aattttcggg
   113701 aagcgtttac gcagcgcgtc gatcacatca gacgtgtcat ccaccgacag cgtggtttgg
   113761 gtcataaagg agagcttctc ttcgtttttg accgtcagtt tccacacatc gtctggcgat
   113821 tcgaccagat acattccccc ttcggggttg ctgtactgcc ccatcgtccc ttccacttcc
   113881 gggtgcccgg cgtggccgat gagaatagat tcttcgccac ggcgactggc gcgggcaact
   113941 tccatatgca ctttggtcac cagcggacag gtggcatcaa acaccgtcaa atcgcggctt
   114001 ttcgcttcgt tacgaaccgc ctgagaaaca ccgtgtgcgg agaaaatcag gatcgcgccg
   114061 tccggcactt cgctaatctg ctcaataaag atagccccac gctcgcgcaa gctatcgacc
   114121 acgtagcggt tatgcaccac ttcgtgacgg acatatatcg gtgcgccgta aatggccagc
   114181 gcgttttcaa caatgctgat agcgcggtct accccggcac aaaaaccacg cgggttggcc
   114241 aacaggatct gcatgttacg cctccagtgc cggatcgatt tccagcactt caatatcaaa
   114301 atgaacggtc tgcccggcca gcggatggtt gaaatcaacg gtaatggagt cgccgttaat
   114361 ttcgcggatc acaccaggca tctcactgcc atccattgcg gtaaaaagca tgattgcgcc
   114421 aatttctggc tcgcctgcat ccataaattc acggcgggag aagtactgaa tcaggtccgg
   114481 tgacggcacg ccaaacgccg catctggctc cagcgagaag gtggttttgt cgcccacttt
   114541 cagccccagc aggtgttgct ccagcccttc agaaagagaa gcatcgccca ggcggaacag
   114601 cgccggttta ccgttgttgc gagtagactc ggcggtggtg ccatcgtcga gttttagcgt
   114661 gaagtgcacc aggacggcgc tattgctctg tacagattca gacatgcagg tttgctctta
   114721 tttttgagga tttttgccgg atgcggccta cgatttcgta gcaatctgta ggccgcatcc
   114781 ggcagggttt attattgttt tttcgctttg gaaggtaaaa acccttccag cacgatcagt
   114841 gccgcaccga cacaaatggc agtatcggca aggttgaagg tggcgaagtg ccagtcgccg
   114901 acgtagaagt cgatcatatc gacaacgaag ccgtgccaca ggcggtcgaa caggttgccc
   114961 agcgcgccgc caataatcag cgcgtaagcg atattgttta gcttctgcgt ggccttcgag
   115021 cgatacatca tcactgccag gagcacgcta ataccaatcg caataccggc aaagaaccaa
   115081 cgctgccagc cgccgctatc ggcaaggaaa ctaaacgccg cgccatagtt acgcgcataa
   115141 tgcagattaa gcgacgggaa cagcgggacc gtatccccca gagcaaagtt ctggaggatc
   115201 aggtatttgc tgcccagatc gataatcagc acgactacca ccagccacag ccagcgtagc
   115261 cctgttgaac agatcgattg actcatcagg caaacttacg tttttcaccg tcaccggcga
   115321 cgttgctgac acagcggccg cagatttctg cgtgttccgc caccttgccg acatcctggg
   115381 tgtagtgcca gcagcgtggg cacttctcac cttcggcttt actcaacgcg actttcagcc
   115441 ctttgagtac ttcgctctgc tgagcatcag caggtgcgtc gttatagtct gcaacggtag
   115501 cgccggaggt caacaggaca aatcgtaatt catcgcccag cgcggtcagt ttcgccgcca
   115561 gttccggttc tacatacaag gttactgccg cttccagcga gccacccact ttcttgtcgg
   115621 cacgcgcttg ctcaatgact ttgttcactt cgccacgcac tttcaacagc tcgtcccaga
   115681 acgcatcgtt cattgcttca ctgtctgcca gaccaaacag gccttcgtac cactcaccgg
   115741 tgaagacgta tttttcacgt tcgcccggca gatagcccca cacttcatca gcggtgaagg
   115801 agaggattgg tgccatccag cgcaccagcg cttcagcgat gtgatacagc gcagtctggc
   115861 agctacgacg cgccacgcta tccgctttgg cggtgtactg acggtctttg atgatgtcga
   115921 ggtagaagga acccatctca acggagcaga agcgcatcag acgctgtacc acttcgtgga
   115981 aatcgtatgc ttcgtacgcc ttgaggatgt cttcctgtgc cgctttcgca caacctacgg
   116041 cccagcgatc cagtaccacc atctcttccg gtttcaccat atcttttgct ggatcaaaac
   116101 cattcaggtt tgccagcagg aagcgcgcgg tgttacggat acgacgatag ctatcggcag
   116161 cacgtttcag gatctcgtca gaaacggcca tttcaccggt gtagtcggtt gatgccaccc
   116221 acagacgcag aatatccgcg cccagtttgt tcatcacatc ctgcggcgaa acggtgttgc
   116281 cgatggattt agacatcttg cgaccctgac catccacggt aaagccgtgg gtcagtacct
   116341 gacgatacgg cgctttgcct ttcatcgcgg tggagatcat tagggaagac atgaaccaac
   116401 cacggtgctg gtcagaacct tccagataca tgtccgctgc gtgaccggca aattccggac
   116461 gcacgtcaac aacagaagag tgggtagatc cggagtcaaa ccatacgtcg agagtatccg
   116521 gcactttcac atactggtca gcttcgtcgc cgaggatctc tttcgcatcg agatcccacc
   116581 acgcctggat gccgtcaact tcaacgcgtt tagccacttc ttccatcagt tcgagggtac
   116641 gcggatgcag ctcttccgtg tctttgtgca cgaacagtga catcggtacg ccccaggtgc
   116701 gctgacggga gatacaccag tcaggacggt tagcaaccat cgactcgata cgcgcctggc
   116761 cccagtccgg gatccactgc acgcctttga tctctttcag tgactgcgca cgcagacctt
   116821 tctgatccat gctgacgaac cactgcggtg tcgcgcggaa gatgatcggc gttttgtgac
   116881 gccagcagca cggatagctg tgctgcattt tctcaacgtg cagcagcgcg cctttttcct
   116941 gcagcagcgc aacgacgatg tcgttcgctt tgaagacgtt cacgccatcc agcgtcggat
   117001 aagtgcccgg cagataagtg ccgtccgggc caaccgggtt agcggtttcc aggccgtatt
   117061 tctgaccgat cacatagtcg tccgggccgt ggccaggcgc ggtgtgaacg gcaccggtac
   117121 cggcatccag ggtaacgtga tcgccgagga ttgccggaac gtcgaagccc ataaacggat
   117181 gggtaaagcg cagcagctca agctctgcac cttttaccgt gccgagaatg gtgtaatcgg
   117241 tcacgccgat acgctgcatt acgctttcaa ccagatcttt cgccagaatc acggcctgac
   117301 cgtcgatctg caccagcgca tagtcgaaat caggtgcaat agagattgcg cggttcgcag
   117361 gcagagtcca cggcgtagtg gtccagatca ccagcgagat tgggccgtta acgttgctta
   117421 cgccaaattt cgctttcagc gcatcctgat cgaccgcctg gaaagcgacg tcaatggacg
   117481 gagaagtttt gtcgtaatac tcaacttccg cttctgccag tgcagaacgg cagtctacgc
   117541 accagtgcac cggcttcgcg cctttgtgca ggtgaccatt gccgatgatt ttgcccagcg
   117601 cgcggatgat gttggcttca gttttgaagt ccatagtcag atacgggtgc gaccagtcgc
   117661 ccagcacgcc cagacggata aagtctttgc gttgaccgtc aacctgggtc gccgcgtatt
   117721 cgcggcactt ggcgcggaac tcggcggcgg tgaatttctc acccggctta ccgtattctt
   117781 gctctacttt cagctcgatc ggcagaccgt ggcagtccca gccaggcaca tacggcgagt
   117841 cataaccgga aagccctttg gacttcacga taatgtcttt cagaatcttg ttaaccgagt
   117901 gaccaatatg aatgctgcca ttcgcataag gagggccatc atgcagaatg aaggtttttt
   117961 tgcctttttt agccgcacgg atgatgccgt acagatcatc atcagtccaa cgcgccagca
   118021 ttcccggttc gcgcttggcg agatcgccac gcatcgggaa ccctgtttcc ggcaaattca
   118081 gggttgattt atagtcactc atcagattct cggttccgtg tttcggtttg attacataac
   118141 aggcttaagc cggttttgtt agcccaaaaa attcgcgggc ggttaattca tcacgcgcaa
   118201 tctgcgcttt cagttcgtcc agcgacgcaa atcgctgctc attgcgtatt tttttacgca
   118261 gcactacttg tatatggcga ccgtaaaggt ccattgcaac atctaacaaa tgcacttcca
   118321 gctgctggcg aataccggca accgttgggc gtgttccgat gtttgccacg ccgggtaacg
   118381 gcttttcacc gaggcccagc acttctaccg cataaacccc tttcaccggg gaaacctgac
   118441 ggcgtagcgg tacattcgcc gtcgggaaac ctatagtgcg ccctaattca tcaccgtgga
   118501 ctacacgccc ggagatggca aacgggtgcc ccagtaaact ttctgccaga gccagattgt
   118561 catcagccag cgcctgacgc acggcggtgc tgctgatacg cacaccacct tcgcaaaaag
   118621 tttgcgtact ggtgatatcg aagccgtatt ccatgccagc tttctgtaat aacaagaaat
   118681 cgccttcacg accagcgcca aagcggaaat catcacctac ggcaagaaat tttacgcgca
   118741 aatgcttcac caaaagatcg ctgatgaaat tttgcgcggt taacgccgca aaacgcctgt
   118801 cgaaacgcac gcacagcacg taatcaacgc cacactctgc aaggtaacgc agtttttccc
   118861 gcagccgggt cagtcttgcc ggggctttat cggtagcaaa cagttccagt ggttgaggtt
   118921 caaaaagcat caccatcacc ggtaagttgc gcttgcgccc ttcttcctgc aagccctgta
   118981 acagcgcgcg atgaccgcga tgcacgccgt cgaaattacc aatagtcagc acacaccctt
   119041 cttgcggggc ctggctgaga ttatgtatgc cgcgtatcag cttcatgtct ggctcaaaac
   119101 agtgaaaatc gtccgagtat accttgtaca gcggtcaagg ttaaccggcg attgagtacc
   119161 gaaatcagaa agaagtggct tttcattgcc atggcgcaaa tcacgggaag aaactgaccg
   119221 cctgctgcaa tttttatcgc ggaaaagctg tattcacacc ccgcaagctg gtagaatcct
   119281 gcgccatcac tacgtaacga gtgccggcac attaacggcg cttatttgca caaatccatt
   119341 gacaaaagaa ggctaaaagg gcatattcct cggcctttga attgtccata tagaacacat
   119401 ttgggagttg gaccttggct aatatcaaat cagctaagaa gcgcgccatt cagtctgaaa
   119461 aggctcgtaa gcacaacgca agccgtcgct ctatgatgcg tactttcatc aagaaagtat
   119521 acgcagctat cgaagctggc gacaaagctg ctgcacagaa agcatttaac gaaatgcaac
   119581 cgatcgtgga ccgtcaggct gctaaaggtc tgatccacaa aaacaaagct gcacgtcata
   119641 aggctaacct gactgcacag atcaacaaac tggcttaatc gccaatttgc tgaagctttg
   119701 tgaaaaagcc cgcgcaagcg ggttttttta tgcctgctgc ttttgtgatg cgtgagagaa
   119761 cacactgccg gttgccagat agtcaaaaga gacatgccgt tgtactatct gaactcttcg
   119821 tggcttaaga atcgtagaac catcggctga tacattctga acaagaatat ctactcaatc
   119881 tccagactcc cccacaaaga atatggatat tgtgatacac attgaggtat taaacttaag
   119941 accactctat ttttgaatta aattcctata tactttaacc attcataaat atctcctcat
   120001 tagacc
//
LOCUS       DADLSB010000002        84680 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.002, whole genome shotgun sequence.
ACCESSION   DADLSB010000002 DADLSB010000000
VERSION     DADLSB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 84680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.002"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..869)
                     /gene="glxR"
                     /locus_tag="JN331_000102"
     CDS             complement(<1..869)
                     /gene="glxR"
                     /locus_tag="JN331_000102"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY7258349.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANH"
     gene            complement(969..1745)
                     /gene="hyi"
                     /locus_tag="JN331_000103"
     CDS             complement(969..1745)
                     /gene="hyi"
                     /locus_tag="JN331_000103"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY7258350.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWIGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1758..3539)
                     /gene="gcl"
                     /locus_tag="JN331_000104"
     CDS             complement(1758..3539)
                     /gene="gcl"
                     /locus_tag="JN331_000104"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY7258351.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGDIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3629..4444)
                     /gene="allR"
                     /locus_tag="JN331_000105"
     CDS             complement(3629..4444)
                     /gene="allR"
                     /locus_tag="JN331_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY7258352.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4522..5004)
                     /gene="allA"
                     /locus_tag="JN331_000106"
     CDS             complement(4522..5004)
                     /gene="allA"
                     /locus_tag="JN331_000106"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY7258353.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELSFA
                     "
     gene            5234..6160
                     /gene="allS"
                     /locus_tag="JN331_000107"
     CDS             5234..6160
                     /gene="allS"
                     /locus_tag="JN331_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY7258354.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNSQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6229..7323
                     /gene="mnmH"
                     /locus_tag="JN331_000108"
     CDS             6229..7323
                     /gene="mnmH"
                     /locus_tag="JN331_000108"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY7258355.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(8034..10448)
                     /locus_tag="JN331_000109"
     CDS             complement(8034..10448)
                     /locus_tag="JN331_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7258356.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(10445..11131)
                     /locus_tag="JN331_000110"
     CDS             complement(10445..11131)
                     /locus_tag="JN331_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7258357.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            11102..11725
                     /gene="tesA"
                     /locus_tag="JN331_000111"
     CDS             11102..11725
                     /gene="tesA"
                     /locus_tag="JN331_000111"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HAY7258358.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            11715..12524
                     /gene="ybbO"
                     /locus_tag="JN331_000112"
     CDS             11715..12524
                     /gene="ybbO"
                     /locus_tag="JN331_000112"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7258359.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            12585..13439
                     /gene="cnoX"
                     /locus_tag="JN331_000113"
     CDS             12585..13439
                     /gene="cnoX"
                     /locus_tag="JN331_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY7258360.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPKEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(13502..14281)
                     /gene="fetB"
                     /locus_tag="JN331_000114"
     CDS             complement(13502..14281)
                     /gene="fetB"
                     /locus_tag="JN331_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY7258361.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(14268..14945)
                     /gene="fetA"
                     /locus_tag="JN331_000115"
     CDS             complement(14268..14945)
                     /gene="fetA"
                     /locus_tag="JN331_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY7258362.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            15091..16008
                     /locus_tag="JN331_000116"
     CDS             15091..16008
                     /locus_tag="JN331_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY7258363.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            16005..16463
                     /locus_tag="JN331_000117"
     CDS             16005..16463
                     /locus_tag="JN331_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY7258364.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(16464..16871)
                     /locus_tag="JN331_000118"
     CDS             complement(16464..16871)
                     /locus_tag="JN331_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY7258365.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(16996..18288)
                     /locus_tag="JN331_000119"
     CDS             complement(16996..18288)
                     /locus_tag="JN331_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7258366.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPCNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(18291..19223)
                     /gene="glsA"
                     /locus_tag="JN331_000120"
     CDS             complement(18291..19223)
                     /gene="glsA"
                     /locus_tag="JN331_000120"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY7258367.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            19485..21989
                     /gene="copA"
                     /locus_tag="JN331_000121"
     CDS             19485..21989
                     /gene="copA"
                     /locus_tag="JN331_000121"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY7258368.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITRGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(22204..22545)
                     /locus_tag="JN331_000122"
     CDS             complement(22204..22545)
                     /locus_tag="JN331_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7258369.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            22683..23477
                     /locus_tag="JN331_000123"
     CDS             22683..23477
                     /locus_tag="JN331_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258370.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            23681..24160
                     /gene="ybaK"
                     /locus_tag="JN331_000124"
     CDS             23681..24160
                     /gene="ybaK"
                     /locus_tag="JN331_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY7258371.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(24197..25849)
                     /locus_tag="JN331_000125"
     CDS             complement(24197..25849)
                     /locus_tag="JN331_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7258372.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEYGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNNPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            26067..27287
                     /locus_tag="JN331_000126"
     CDS             26067..27287
                     /locus_tag="JN331_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7258373.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            27525..29201
                     /locus_tag="JN331_000127"
     CDS             27525..29201
                     /locus_tag="JN331_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY7258374.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(29334..30638)
                     /locus_tag="JN331_000128"
     CDS             complement(29334..30638)
                     /locus_tag="JN331_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY7258375.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPSHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNIPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            30790..31749
                     /gene="aes"
                     /locus_tag="JN331_000129"
     CDS             30790..31749
                     /gene="aes"
                     /locus_tag="JN331_000129"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAY7258376.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(31746..32708)
                     /gene="hemH"
                     /locus_tag="JN331_000130"
     CDS             complement(31746..32708)
                     /gene="hemH"
                     /locus_tag="JN331_000130"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY7258377.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDEALKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(32840..33484)
                     /gene="adk"
                     /locus_tag="JN331_000131"
     CDS             complement(32840..33484)
                     /gene="adk"
                     /locus_tag="JN331_000131"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY7258378.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(33665..35539)
                     /gene="htpG"
                     /locus_tag="JN331_000132"
     CDS             complement(33665..35539)
                     /gene="htpG"
                     /locus_tag="JN331_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY7258379.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(35649..36254)
                     /gene="recR"
                     /locus_tag="JN331_000133"
     CDS             complement(35649..36254)
                     /gene="recR"
                     /locus_tag="JN331_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY7258380.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(36254..36583)
                     /locus_tag="JN331_000134"
     CDS             complement(36254..36583)
                     /locus_tag="JN331_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY7258381.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     NINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(36636..38561)
                     /gene="dnaX"
                     /locus_tag="JN331_000135"
     CDS             complement(36636..38561)
                     /gene="dnaX"
                     /locus_tag="JN331_000135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY7258382.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            complement(38690..39241)
                     /gene="apt"
                     /locus_tag="JN331_000136"
     CDS             complement(38690..39241)
                     /gene="apt"
                     /locus_tag="JN331_000136"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY7258383.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(39394..39771)
                     /locus_tag="JN331_000137"
     CDS             complement(39394..39771)
                     /locus_tag="JN331_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY7258384.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39841..40368
                     /gene="priC"
                     /locus_tag="JN331_000138"
     CDS             39841..40368
                     /gene="priC"
                     /locus_tag="JN331_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY7258385.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            40382..40543
                     /gene="rsmS"
                     /locus_tag="JN331_000139"
     CDS             40382..40543
                     /gene="rsmS"
                     /locus_tag="JN331_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY7258386.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(40755..44117)
                     /gene="mscK"
                     /locus_tag="JN331_000140"
     CDS             complement(40755..44117)
                     /gene="mscK"
                     /locus_tag="JN331_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY7258387.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(44245..44892)
                     /gene="acrR"
                     /locus_tag="JN331_000141"
     CDS             complement(44245..44892)
                     /gene="acrR"
                     /locus_tag="JN331_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY7258388.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            45034..46227
                     /gene="acrA"
                     /locus_tag="JN331_000142"
     CDS             45034..46227
                     /gene="acrA"
                     /locus_tag="JN331_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY7258389.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            46250..49399
                     /locus_tag="JN331_000143"
     CDS             46250..49399
                     /locus_tag="JN331_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY7258390.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFANDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            49945..50319
                     /gene="tomB"
                     /locus_tag="JN331_000144"
     CDS             49945..50319
                     /gene="tomB"
                     /locus_tag="JN331_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY7258391.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     LGNRLFRCFVNATKENPASLSC"
     gene            50345..50563
                     /gene="hha"
                     /locus_tag="JN331_000145"
     CDS             50345..50563
                     /gene="hha"
                     /locus_tag="JN331_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7258392.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            50735..51286
                     /gene="maa"
                     /locus_tag="JN331_000146"
     CDS             50735..51286
                     /gene="maa"
                     /locus_tag="JN331_000146"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY7258393.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            51402..51872
                     /locus_tag="JN331_000147"
     CDS             51402..51872
                     /locus_tag="JN331_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258394.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            52036..53586
                     /gene="pdeB"
                     /locus_tag="JN331_000148"
                     /pseudo
     CDS             52036..53586
                     /gene="pdeB"
                     /locus_tag="JN331_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(53628..53981)
                     /locus_tag="JN331_000149"
     CDS             complement(53628..53981)
                     /locus_tag="JN331_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY7258395.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(54101..54197)
                     /gene="ffs"
                     /locus_tag="JN331_000150"
     ncRNA           complement(54101..54197)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JN331_000150"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            54360..54671
                     /locus_tag="JN331_000151"
     CDS             54360..54671
                     /locus_tag="JN331_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY7258396.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(54702..55274)
                     /locus_tag="JN331_000152"
     CDS             complement(54702..55274)
                     /locus_tag="JN331_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258397.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAINVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            55492..56352
                     /gene="tesB"
                     /locus_tag="JN331_000153"
     CDS             55492..56352
                     /gene="tesB"
                     /locus_tag="JN331_000153"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY7258398.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(56401..57687)
                     /gene="amtB"
                     /locus_tag="JN331_000154"
     CDS             complement(56401..57687)
                     /gene="amtB"
                     /locus_tag="JN331_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY7258399.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATEAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(57717..58055)
                     /gene="glnK"
                     /locus_tag="JN331_000155"
     CDS             complement(57717..58055)
                     /gene="glnK"
                     /locus_tag="JN331_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY7258400.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(58236..60017)
                     /locus_tag="JN331_000156"
     CDS             complement(58236..60017)
                     /locus_tag="JN331_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7258401.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(60010..61782)
                     /locus_tag="JN331_000157"
     CDS             complement(60010..61782)
                     /locus_tag="JN331_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7258402.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAVDAKDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFVLKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(61812..62357)
                     /locus_tag="JN331_000158"
     CDS             complement(61812..62357)
                     /locus_tag="JN331_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAY7258403.1"
                     /translation="MVFKPHRINCRDFTFKIELKEKKILRGRAMLDKIDRKLLALLQQ
                     DCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKVALLDPEKIGLGLTAFVLIKTQ
                     HHSSEWYCRFVTVVTEMPEVLGFWRMAGEYDYLMRVQVADMKRYDEFYKRLVNSVPGL
                     SDVTSSFAMEQIKYTTSLPIE"
     gene            complement(62424..63242)
                     /gene="cof"
                     /locus_tag="JN331_000159"
     CDS             complement(62424..63242)
                     /gene="cof"
                     /locus_tag="JN331_000159"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY7258404.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATYCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            63342..65042
                     /locus_tag="JN331_000160"
     CDS             63342..65042
                     /locus_tag="JN331_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAY7258405.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPS
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            65107..65802
                     /gene="queC"
                     /locus_tag="JN331_000161"
     CDS             65107..65802
                     /gene="queC"
                     /locus_tag="JN331_000161"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY7258406.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(65854..66252)
                     /locus_tag="JN331_000162"
     CDS             complement(65854..66252)
                     /locus_tag="JN331_000162"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY7258407.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(66346..66717)
                     /locus_tag="JN331_000163"
     CDS             complement(66346..66717)
                     /locus_tag="JN331_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258408.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(66868..68739)
                     /gene="ppiD"
                     /locus_tag="JN331_000164"
     CDS             complement(66868..68739)
                     /gene="ppiD"
                     /locus_tag="JN331_000164"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7258409.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(68931..69203)
                     /gene="hupB"
                     /locus_tag="JN331_000165"
     CDS             complement(68931..69203)
                     /gene="hupB"
                     /locus_tag="JN331_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY7258410.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(69412..71766)
                     /gene="lon"
                     /locus_tag="JN331_000166"
     CDS             complement(69412..71766)
                     /gene="lon"
                     /locus_tag="JN331_000166"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY7258411.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(71954..73228)
                     /gene="clpX"
                     /locus_tag="JN331_000167"
     CDS             complement(71954..73228)
                     /gene="clpX"
                     /locus_tag="JN331_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY7258412.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(73354..73977)
                     /gene="clpP"
                     /locus_tag="JN331_000168"
     CDS             complement(73354..73977)
                     /gene="clpP"
                     /locus_tag="JN331_000168"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY7258413.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(74224..75522)
                     /gene="tig"
                     /locus_tag="JN331_000169"
     CDS             complement(74224..75522)
                     /gene="tig"
                     /locus_tag="JN331_000169"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY7258414.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(75866..76183)
                     /gene="bolA"
                     /locus_tag="JN331_000170"
     CDS             complement(75866..76183)
                     /gene="bolA"
                     /locus_tag="JN331_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY7258415.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            76488..77066
                     /locus_tag="JN331_000171"
     CDS             76488..77066
                     /locus_tag="JN331_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7258416.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            77110..78585
                     /gene="ampG"
                     /locus_tag="JN331_000172"
     CDS             77110..78585
                     /gene="ampG"
                     /locus_tag="JN331_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY7258417.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            79045..79992
                     /gene="cyoA"
                     /locus_tag="JN331_000173"
                     /pseudo
     CDS             79045..79992
                     /gene="cyoA"
                     /locus_tag="JN331_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
     gene            80014..82005
                     /gene="cyoB"
                     /locus_tag="JN331_000174"
     CDS             80014..82005
                     /gene="cyoB"
                     /locus_tag="JN331_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HAY7258418.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGASANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNCDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            81995..82609
                     /locus_tag="JN331_000175"
     CDS             81995..82609
                     /locus_tag="JN331_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY7258419.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMKIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            82609..82938
                     /locus_tag="JN331_000176"
     CDS             82609..82938
                     /locus_tag="JN331_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY7258420.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            82950..83840
                     /gene="cyoE"
                     /locus_tag="JN331_000177"
     CDS             82950..83840
                     /gene="cyoE"
                     /locus_tag="JN331_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY7258421.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            84025..84336
                     /locus_tag="JN331_000178"
     CDS             84025..84336
                     /locus_tag="JN331_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7258422.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSVAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            84320..>84680
                     /locus_tag="JN331_000179"
     CDS             84320..>84680
                     /locus_tag="JN331_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HAY7258423.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVM
                     LGGARKYQKRGFGQDLLC"
BASE COUNT        19998 a        22508 c        21397 g        20777 t
ORIGIN      
        1 ttatggttag ccattaattc cagcgcctgc actaacgcag agtgatccaa ctggctgcca
       61 ccgtttgccg cacaggtatt aaataactcc tggcaggtcg cagtgtttgg caggttcagc
      121 gcaagtgctt tcgcactttg cagtgccagg ttgagatctt tctggtgcag agcgattttg
      181 aagcccggat taaaggtgcg tttaatcatg cgctcaccgt gaacttccag aatacgtgag
      241 gaagcaaagc cgcccatcag cgcctggcgc acacgtaccg ggtccgcacc ggcttttgaa
      301 gcaaatagca gggcttcaga aaccgcttca atattgagcg ccacaataat ctgatttgcc
      361 actttgcagg tttgaccatc gccgttaccg cccacgaggg tgatattttt accgagcagt
      421 tcaaacagcg gtttaacacg ttcaaatacc gcttcatcac cgccaaccat aatcgacaac
      481 gtcccttcac gcgcaccgat ttcaccgccg gagactggcg catcgagata atcgccgccc
      541 agttcattca cctgacgagc gaaacgctta gtttcaatcg gggaaatgga gctcatatca
      601 acaatggttt tgcccttcag cgaggctttg gtacaaccat tttcaccgaa cagaacttct
      661 tcaacctgag gtgtgtccgg caccataata aaaatgatgt ccgatgcttc cgttacctgg
      721 cgagcagttt caacactgac ggcacccagt gacagtaatt catcagcaac cggtccaatg
      781 gtcgtgacat gtaattgatg accggcacgc gccagattaa tggccatcgg tgtacccata
      841 atgcctaagc caataaatcc cagtttcata attaacctct tttaaatttc gcttttcctg
      901 aattcagaca acacgatccc ctgccaactt agcggcctaa aaaagcattg tctgaatagc
      961 gttacgtttt aacggtacgg atccatccag cgtaaaccag cttccgtggt ggtttggggt
     1021 ttatattcac acccaatcca accgttgtag tcagagtttt caattaccgt aaagagatat
     1081 tcgtaattaa tttctccggt tcccggttcg ccgcgatgcg gattatcagc aatttgcagg
     1141 tgaccaattt tatcggccca ctgagtcatg gtgttggtta attcaccttc catccgctgc
     1201 atgtgataaa tgtcatactg aattttcaaa ttgcagcaac caacatcatc aatcaatttc
     1261 agcgcctgcc gagttccggt gagatggaaa ccaggaatat caaaatggtt aataggttca
     1321 atcagtaata aaatatcttc tttcatcagc atattcgcgg cataacgcag gttttctaca
     1381 agcgttgcgt gaatctgttc actgctgaaa ccagccggcg ttttaccgac cagacagtta
     1441 atttttttat tacccagcgc acgggcataa cgaatcgctg ctgctacgcc atcccgaaac
     1501 tcttcttcac ggccaggaat acaggcaata ccgcgctcgc ccgccgccca gtcacctgcc
     1561 ggtaaattgt gcagcgtatg ttcgagttta ttactcgcca gtacctgttt taattcttca
     1621 atgtcgtagt cataaggaaa cataaattca acgccgcgaa aaccacactg cgcagctttc
     1681 tcaaaacggg cgagaaaatc atattcacca aataacatcg ataaattagc agagaaacgt
     1741 aacatcattt atctccctta ttcatagtgc atgaagcagg tttcagtcgg tgcatccgct
     1801 gcgttatcgg cgatatcttc aaattccatg acgttatcca gttcgctgcc catcgaaata
     1861 ttggtcacac gctcgagaat aacttccacg actaccggta cccgatattg cgccattaag
     1921 gctttcgcct gttcaaaggc tggcgcaata tcttccggtt tgaagacccg aatagcttta
     1981 caacctaaac cttccgctac ttttacgtgg tcaacaccgt agccattcac ttcactggag
     2041 ttgatattct cgaaagcgag ttgcacgcag tagtccatgt caaaagcgcg ttgtgactga
     2101 cgaatcaggc cgagataggc gttattcacc agcacatgga tgtacggaat gttgaactgc
     2161 gcgccaacgg ctaactcttc aatcaggaac tggaagtcaa agtcgccaga aatcgccacc
     2221 acattgcgtt tcggatcagc ggcacaaacc cccagcgccg ccggaatcgt ccagcctaac
     2281 ggaccagcct gaccacagtt gatccagtgg cggtctttaa agacatgcag catttgtgcc
     2341 gcagcgattt gtgacagacc aatagtggtg acataacaaa catcgcgacc aaaggctttg
     2401 ttcatctctt catacacgcg ctgcggtttc accggcacgt tgtcgaagtg ggttttgcgc
     2461 agcaaagtgc gtttacgctg ctggcagtcg gcgacccatt ctttacgaca cggcagacga
     2521 cccgcttttt gcatctcctg cgccacttca accagcagtg tcagcgccgc tttagcatca
     2581 gagacaatgc cgagatccgg acacagcacg cgaccaattt gcgtcggctc aatatcaata
     2641 tgaacgattt tgcgcccttc ggtgtatttc tctaccgaac cggtatgacg gttagcaaaa
     2701 cggttaccga taccaaacac catgtcggac gccagcaacg ttgcgttacc gtaacgatgc
     2761 gcggtttgca gaccgaccat cccggccatc agttcatgat cgtccgggat acagccccag
     2821 cccatcagcg ttgggatcac cggaacgctg gtcagttcag caaactgttg taacagtgca
     2881 gctgcgtcag cattgattac cccgcccccg gcaacaatca ccggacgttc ggcctggatt
     2941 aacatttcta cagctttttc gatctgcata cggctggcag caggtttgta gaccggcagc
     3001 ggttcgtaca tgtcaggatc aaactcgatt tccgcaacct gaacgtcgaa cggtaaatcc
     3061 accagtaccg gacccggacg accagaacgc atcaggtgaa atgcctgttg cagcacgcga
     3121 ggcaccagcg ccgcttcacg aactgtaacc gccattttgc tgaccggttt agcaattgct
     3181 tcaatatcta cggcctgaaa atcttcttta tgcagacggg cgcgcggtgc ctggccggta
     3241 atgcacagaa taggaatgga atcagcagaa gcggaataga gcgcggtgat catgtccgtg
     3301 cccgcaggac cggaagtccc cagacatacg ccgatatccc ctgccgttgc gcgggtataa
     3361 ccttccgcca tgtgcgaaac accttccaca tgacgcgcca gaatgtgacg aataccgccg
     3421 tgcttacgca tcgctgagta gaacggatta attgcagctc ccggaacgcc gaaggcggtg
     3481 gtgatacctt ctttctccag cacatacatt gccgcgtcaa cggctctcat ttttgccatt
     3541 ttattcctac ctctattaat tggaaaaatt ttccaacttt caaattttgt caaaaaaacc
     3601 cgctccgcag agcgggatgc gacagacact atggatgtgc tttcagtccc aacgccgtgc
     3661 tgatatcgcg ggcggtgtct ctgaccagct caccctgact gacaaaacga tcttctgtca
     3721 gtcttgatga aggcccggag atggagatag cggcaacaac gctaccgaca tcatcgtaaa
     3781 ttgctgaagc tatgcaattc agacctacaa catgctcttc tttatctacg gtatagccca
     3841 gttcacgcgc ttgttccagg tccttcagca aggtgggcat atccacaagc gtagttggcg
     3901 taaactgctg caaaccggtt tgcagaatga tgctcatcaa ctcctcttcc gccagcggat
     3961 aaagcagcgc tttgcccgca ccggaagcat gcagcggcag acgactgccc agtggcgcac
     4021 acatcctgac catcgattta cactctaact gaccaattaa taccgcttca ttgccgttac
     4081 ggatcgcgac attgaccgtt tcgccggaaa gtaacatcag gcggcgcata aacggcccgg
     4141 cgacggagag gacatcgcgg ttatggatgt acgccgcacc gacgttaaag acacctaatc
     4201 ctatatgcca ccagcctaat tgactgtcct gatagacaaa atccgctgct tgtaaaacct
     4261 tcagcaagcg aaaggtcgtg gagagcggca aatccagatt gagagaaata tcgctaaccg
     4321 acgaacttcc cccacttttt tccagatatt gcagaatcgc aattccccgc tctaacgcct
     4381 gtgcgccctt ctgtgccaca ggctccgcct gtcctggcct gccgcgccgt ctaacttccg
     4441 tcatacctac gttaactccg acctacaagt gagcataaac aatgaacaat tagtttatct
     4501 tatgcaagtc ggttgcaggc gtcacgcaaa actgagttcc tgttcaggaa tactttcaac
     4561 atcacagttg tcactgccgc cgcgatcgat ggtcagaaaa tcggtgacgc gctgccaggc
     4621 gaaaagtggg tgatgccaga cgttacgatg gtaattcacc ccctgttcac cgttggtgat
     4681 aaacgcccgc agcgttgaca ggtctggttt gtcgtcgcct aacgccacga ccaccacaaa
     4741 cacttcacct ttcattggga taaaggcctg agtgcccagc ggatgacgtt cgagttcgtg
     4801 aatggtcagc ggcagattcg ccggttgcgc gcggttaatg ctgataagcg tacggtcttg
     4861 ctcaagaatc tcaaccagcg ccaaatcgtg gtaacgctcc accaggccat tgttaatatg
     4921 gaaaaaatcc cgttgctgcg tttcgattac gtcgccataa gcactaaagg cttcctgact
     4981 taacggtaat acctgaagtt tcatcttttt gtccttgtaa cataattccg ttcaaccacc
     5041 gccgaccggt cagcttggtt tcaataacag gcaatctaaa accttaagag aacaacatca
     5101 atacaaaagt tggaatgttt ttccaaaact agatttagtc cacaaattga acagggctat
     5161 catgtaaaaa tactgataaa tcagaggata agaaaatcac gcaacttgcg gatttaaaag
     5221 gggatacctg aagatgttcg atccagaaac cttgcggact ttcattgcgg ttgctgaaac
     5281 aggaagtttt tcaaaagcgg cagaacgatt atgtaaaacc acggcgacga tcagttatcg
     5341 cattaaactt ctggaagaga ataccggagt agcgctgttt ttccgtacga ctcgtagcgt
     5401 aacgttgaca gcggctggcg agcatctact ttcccaggcc agagactggc tgagctggct
     5461 ggaaagtatg cccagcgagc tgcaacaggt gaatgatggc gtggaacgcc aggtgaatat
     5521 tgtcatcaac aacctgctct ataactccca ggccgtcgcc cagttgctgg cgtggctgaa
     5581 tgagcgttac ccctttaccc agtttcacat ctcccgacaa atctatatgg gcgtctggga
     5641 ctcgctattg tacgaaggtt tttcgctggc tatcggggtc acgggaactg aggcgctggc
     5701 aaataccttt agtcttgatc ccttaggatc ggtgcaatgg cgatttgtca tggcggcgga
     5761 tcatccgctg gcgaacgttg aagagccgct aacagaagcg cagttgcgtc gctttccggc
     5821 ggtcaatatt gaagacagcg cccgcacctt aaccaaacgc gtcgcctggc gattgccggg
     5881 gcaaaaagag attattgttc ccgatatgga aacgaaaatc gccgcccatc tggcgggtgt
     5941 tggcattggt tttttgccaa aatcactttg ccagtcaatg atcgataatc aacaactggt
     6001 cagccgggta atcccaacga tgcgccctcc ttcgccattg agtctggcat ggcgcaaatt
     6061 tggcagcggc aaagcggtag aagatattgt gaccttgttt acccaacgca ggccggaaat
     6121 cagcggattt ttagaaattt tcggcaaccc acgcagttaa aaatcaagtt tccttgcgcg
     6181 ttggcgcgta aaatagcgcg caagctgatt tgaggttcac ttttaactat gcaagagaga
     6241 cacacggaac aggactatcg tgccctgctg attgctgata cgcccattat tgatgttcgc
     6301 gcccctatcg agtttgagca aggcgcaatg cccgccgcta tcaatctgcc gttaatgaat
     6361 aacgatgaac gcgccgccgt tggcacctgc tataaacagc aaggctcaga cgcagcgctg
     6421 gcgctggggc ataaactggt ggcgggtgaa attcgtcagc agcgtatgta cgcctggcgg
     6481 gcagcgtgcc tgcaaaatcc gcaaggtatt ctctgctgcg cccgtggcgg tcaacgttca
     6541 catattgtgc aacgctggct gcatgaagcg gggattgatt atccgctggt ggaaggcggt
     6601 tataaggcac tgcgccagac cgcgattcag gcgaccattg aactggcaca aaaaccgata
     6661 gtgctgattg gcggttgtac cggcagcggt aaaacgctat tagtgcaaca acagccaaac
     6721 ggtgttgatc tggaagggct ggcgcgtcat cgcggttcgg cgtttggtcg cacgttacaa
     6781 ccacaactta gccaggcgag ttttgaaaac ctgctggctg ccgaaatgct aaaaaccgac
     6841 gcccgtcagg atttgcgcct gtgggtgctg gaagatgaaa gccggatgat cggttcgaat
     6901 cacctgccgg aatgtctgcg cgagcgaatg actcaggcgg cgattgcggt ggtagaagat
     6961 ccgtttgaga tccgtcttga acgcctgaat gaagagtatt tcttgcgtat gcatcatgat
     7021 tttacccacg cttatggcga cgaacagggc tggcaggaat attgcgaata cctgcatcac
     7081 ggactttcgg cgattaagcg tcggctgggg ttacagcgct ataacgaact cgctgcccag
     7141 ctggatacgg cgctgacaac gcaactcacc accggcagca ccgacggtca tctggcctgg
     7201 ctggtgccgt tacttaaaga atattacgac ccaatgtatc gctatcagct tgagaaaaaa
     7261 gcggaaaaag ttgtctttcg cggtgagtgg gcggaagtgg cggtatgggt taaggcgcag
     7321 tgatgggcgg tattctgaaa cggcgattgt cgtgagaaca aaatggttgc accagaatac
     7381 agatataaaa accgacatga cagtcggttt ttttattttt tccgcccaag acttggtggt
     7441 tccgggcaga aattaaacag tcctcatcta gcaagtgaat tataagtcag gatgcctaca
     7501 tattcaaaca gctatgttgg cgatacaatc ccgacgctag tagtgatact gcctcaaggg
     7561 attaccgcag tgcggaaaag tacttttaag tcctggtcag gatacaggtg gtgctgattc
     7621 tggtgctcta atttggagag aggcagtcct catctagcac atgaagaaaa tcatagttac
     7681 aggcaacgta agggtaagct gtgattgtct ttctccctta agggagcaac ggatgaaaac
     7741 ttttctcgat aaagttctca tcgttcttaa ggcgctaatc gcgctgctgg aactgatccg
     7801 tcagtttatt gaataactga ggaaacgtag ctaaggccga aggtttacct ccttcggcct
     7861 ttaaaaaaac cgacatgaca gttggtttca tatttattgc caaaatgcac actgttcatg
     7921 ccagatgcgg cgtaaacgcc ttatccggcc tacacaatct tgcaaaatca atatattgca
     7981 cgctcttcgt aggcctgata agcgtagcgc atcaggcaat ctaacacctg tcatcacccc
     8041 acaaactgcc tgaacagcgc cttaccctta accagtcgcg cacccagcca gccgccgaaa
     8101 agcgacagca gcagcgcacc gctgcacggc agaacaatcc acaatcgcca gtctggctcc
     8161 cacgggaaat caaacacttt cgcctgcaac actgccagtg ccgtttccgc accaattgcg
     8221 gccaccaggc cggaaacaaa cccaagcatg gcgaactcac accacaatgt cgtgcgcagc
     8281 agttttttcc ccgcaccgag tgtgcgccat accaccagct cctgatgacg ctggcgcatt
     8341 cccacctgca cctgtgccag caacagcaac ataccgcagg cggtgaccag caccaccatc
     8401 acctccagcg cccgacttac ctgctccagc acctgaccga cctgttttaa aatcgcgcca
     8461 atgtctaaca ggctaatggt cgggaactgg cggttgagtt gcgtcaacat gccgttgcca
     8521 ttctcccagc ggaaactggt aagccagctc tgcggttgcc cgtctaatgc cccttcaggg
     8581 aaaataaaat agaaattagg ccgcagactt tcccagtcca ctttgcgcag gctggtcact
     8641 ttagcgcgga actcctgggt atcgcccata aaagtcacgg tatcaccgag ggcaacgttt
     8701 aagcgtttcg ccagcccctc ttccatcgac acttcatcgg cttttggcgg ccagttaccg
     8761 gcgacaatcg gattatgatc gggccgcgta ttttgccagg taagattgag ttcgcgatta
     8821 agcgcctcat cttcattacc ttctgtcggc ttatcgttaa tcgccgtcag ccgcgcccgc
     8881 actaccggat aaaatgattc cgggactata tgatgttccg cgaggaacgc ttttagcggt
     8941 gctacctgtt ctgtggcgat gttaattaaa aagtagttcg ggctttctgg cggtagctgc
     9001 tgctgccagc ggtcgagcag atcgccacgc agcaccagca gcagtgccag cagcataaag
     9061 gagagcgaaa atgccgaaag ctggcttaac gttgaccacg gctgacgtaa caggcggcta
     9121 accgccaggc gcagaggcag cgatttcagc gtcatgcggc gaagtacatt cagcagcatc
     9181 cagcccagca caccgcacag caaagccagt actaccgcgc ccgccagcac cgcccaaagc
     9241 agcatgctgc cacccattaa tccggcgagc agcagcacaa ccaccacact gacaatcggc
     9301 agataaaact tcagcggcca gacgttcgcg accacatcat tacgtaatac gcgtaaaggc
     9361 tgcgttgcca gcaacaagcg gtatggtcgt agccccacca gcagcgagat gaccgtcatg
     9421 gtgccaagcg cccacagcca cggccagagg ctggctggcg gtagtgcagc aggcagaacg
     9481 ggcttgagca gcaccatcaa cacgttttcg aacaacaggc ctatggcccc accggtaacg
     9541 gctgaaagcg tcagcaccat caaccactga ccgacgatta gcttacgcag ttgcgctcgc
     9601 cctgccccca gcgttttgag gatcgccacc agatcgtagc gactgcgaca gtaatgattc
     9661 atcgctaccg ccaccgctgc cactgccagc agcaaggtca gaagcgccga aagcagcagg
     9721 aactgttgcg agcgttccat cgatcgcccc agcgcgcctt cgtcctgttc cagaccgtac
     9781 cagcgttgtt cgggtttaag ctgaggtaac aaccatttct catagccgtc gagctggttc
     9841 tcgttgccgc cgaatttata acgccaggtg acccgactcc ccggctgcac ggctccggtt
     9901 ttatcgacat ccgccagatt catcatcaga cgcggagcca tctggaaggg gttaaaaccg
     9961 gaatccggtt cctgaatcac ttctccggca atccgcaagg tggcatcgcc cacgtcaatg
    10021 gtgtcgcccg ttttcaggtt aagcagtgcc atcaggcgtg gggccagcaa tacgctgccc
    10081 gcctgcggtt tcaggccagg gggattagtt tgcagatcgc catacatcgg gtagatatca
    10141 tccaccgctt tgacgttcgc cagctgcggt gtgtcgcctg caaaggtcat tgtggcgaaa
    10201 gtcagctgct tgccgacttt caggccgcgc ttttgcgctt cctccagcca cgcttgcggc
    10261 acttcgcgtg aactgcgcaa cgcccgatcg cccgccataa actcacggct ttgctggctt
    10321 aagcccttct ccatgcgatc gctgatattg cccagcgcca gcacgcaggc caccgccagg
    10381 cttagcgcca gccagacaat taatagcgac ggcgaacgcc attcgcgcca gaaccaacgt
    10441 gcaatcatgc ttcctcctgc aactgcccgt tcaccagccg taagcagcgg tcgcagcgtg
    10501 ccgccagttg caggtcgtgg gtcaccataa tcaacgtggt gccatgttca cggttgaggg
    10561 aaaacagcag gtcggcaatt ttatcgcccg tctggcggtc aaggttgccg gtgggttcgt
    10621 cggcaaacag cacatcaggt cgaccattaa aggctcgtgc cagcgccact cgttgctgtt
    10681 caccgccgga aagctgcgcc ggaagatgat ccagacgttt acccagccct aactgttcga
    10741 gcaacgcttt cgccccgtta cgactttccg cgctactctc accgcgcagc agagccggaa
    10801 gctcgacgtt ttccagcgcg ttaagggtag gaattaacat aaatgactga aaaacaaagc
    10861 cgacgtgctt cgcgcgcaac tttgcccgcg cttcttcgtc catattatgt agcggttgtc
    10921 ccaccagact cacttcgcca ctgctgccgt catcaagccc ggcgaggatc gccagcaagg
    10981 ttgacttacc cgatcccgac tcgcccacca gtgcgatggt ctcgccacgt ttgacaacca
    11041 gctcaactcc ggtgaggatg gagagttcat gctccccctg accgacggac ttcttaagat
    11101 gatgaacttc aacaatgttt tccgctggca tttgcccttc ctgttcctgg tcctgttaac
    11161 cttccgtgcc gccgcagcgg acacgttatt gattctgggt gatagcctga gcgccgggta
    11221 tcgaatgtct gccagcgcgg cctggcctgc cttgttgaat gataagtggc agagtaaaac
    11281 gtcggtagtt aatgccagca tcagcggcga cacctcgcaa caagggctgg cgcgccttcc
    11341 ggctctgctc aaacagcatc agccgcgttg ggtgctggtt gaactgggcg gcaatgatgg
    11401 tttgcgtggt tttcagccac agcaaaccga gcaaacgctg cgccagattt tgcaggatgt
    11461 caaagccgcc aacgctgaac cattgttaat gcaaatacgt ctgcctgcaa actatggtcg
    11521 ccgttataat gaagccttta gcgccattta ccccaaactc gccaaagagt ttgatgttcc
    11581 gctgctgccc ttttttatgg aagaggtcta cctcaagcca caatggatgc aggatgacgg
    11641 tattcatccc aaccgcgacg cccagccgtt tattgccgac tggatggcga agcagttgca
    11701 gcctttagta aatcatgact cataaagcaa cggagatcct gacaggtaaa gttatgcaaa
    11761 aatcggtctt aattaccgga tgttccagtg gaattggcct ggaaagcgcg ctcgaattaa
    11821 aacgccaggg ttttcatgtg ctggcaggtt gccgaaaacc gaatgatgtt gagcgtatga
    11881 acaacatggg atttaccggc gtgttgatcg atctggattc accagaaagt gttaatcgcg
    11941 cagcagacga ggtgatcgcc ctgaccgata attgtctgta tgggatcttt aacaatgccg
    12001 gattcggcat gtatggcccc ctttccacca tcagccgtgc gcagatggaa cagcagtttt
    12061 ccgccaactt tttcggcgca caccagctca ccatgcgcct gttacccgcg atgttaccgc
    12121 acggtgaagg gcgtattgtg atgacatcat cggtgatggg attaatctcc acgccgggtc
    12181 gtggcgctta cgcggccagt aaatatgcgc tggaggcgtg gtcagacgcg ctgcgcatgg
    12241 agctacgcca cagcggaatt aaagtcagcc tgatcgaacc cggtcccatt cgtactcgct
    12301 tcaccgacaa cgtcaaccag acgcaaagtg ataaaccagt cgaaaatccc ggcatcgccg
    12361 cccgctttac gttgggaccg gaagcggtgg tcgacaaagt gcgccatgct tttattagcg
    12421 agaagccgaa gatgcgctat ccggtaacgc tggtgacctg ggcagtaatg gtgctcgagc
    12481 gcctgctgcc tgggcgcgtg atggacaaaa tattgcaggg gtgagttgaa gcgcgcgctt
    12541 aagcccccat gtcaaagaaa atatcgacaa cagagagcga ctccatgtcc gtagaaaata
    12601 ttgtcaacat taacgaatct aacctgcaac aggttcttga acagtcgatg accactccgg
    12661 tgctgttcta tttttggtct gaacgtagcc agcactgttt gcagttaacc ccgatcctgg
    12721 aaagcctcgc ggcgcagtac aacgggcaat ttattctggc gaagctggac tgcgacgcgg
    12781 agcagatgat tgccgcccag tttggtctgc gcgcgattcc gaccgtgtat ctgttccaga
    12841 acgggcaacc ggtagatggc ttccaggggc cgcaaccgaa agaggcgatc cgcgccctgc
    12901 tggataaagt gctgccgcgc gaagaagagc tgaaagcgca gcaggcgatg caactgatgc
    12961 aggaaggcaa ttacaccgat gccctgccat tgctgaaaga cgcctggcag ttgtcgaatc
    13021 agaacgggga gatcggcctg ctgctggcag aaacgctgat tgcgctgaac cgttctgaag
    13081 atgcggaagc ggtgctgaaa accattccgt tgcaggatca ggacacccgc taccaggggc
    13141 tggtagcgca aatcgaactg ctgaagcagg cggctgataa gccggaaatt caacagttgc
    13201 aacagcaggt ggcggagaat ccagaagatg ccgcactggc gacgcaactg gcgctgcaac
    13261 tgcatcaggt tgggcgcaat gaagaggcgc tggagttgct gttcgggcat ctgcgtaaag
    13321 atctcaccgc cgcagacggt cagacgcgta aaacgttcca ggagatcctc gctgcgctgg
    13381 gtacgggtga tgcactggcg tcgaagtatc gccgccagct gtatgcattg ttgtattgat
    13441 attccgttgt tcggcacatg aaccacaacg ccggatgcga tgctgcgcat ccggcatcgc
    13501 atcatttctt cttcaattga gtcaccacca actggtggcg cgaattataa aacttacgat
    13561 aggttaaata gcaggcaata atggtcgaca agctggcggt tgagagcagc ataaaggtca
    13621 ccataatctg atatttaatc gccttcaccg gatcaatccc ggcaaatatc agcccggaca
    13681 tcattcctgg taaactcact aagccaaccg tttttgctga atcgaccgtc ggaattaaag
    13741 ccgcgcgaat actgtcgcga atcaatatcg ctgaagcctg cttaggcgtc gcgccaagac
    13801 tcagtttctc ctgaatctgt tgctgttcgc taatgacccg ttgccctaaa ttgttgtaac
    13861 acaaccccac cgctaccatg gcgttaccgg caatcatccc ggcgacaggg atcacctgca
    13921 tcgggataaa ttcaatcgac cctgagagaa tcagcaccgc cagggtaatt cccgccccga
    13981 ccgtaatggc aataaacgat gagataaaag ctttagcaat atatttactg cgtttttgcg
    14041 cgttccacgc cgcattaaag cagataaata acaccatcaa taatgtcagg ctggcatcat
    14101 ccacgctgaa aatatacttc agcacatagc cgacaataat cagctgaatt atcgctcgcc
    14161 cgacgctcca gagaatatct ttctccagcg ccagtttttc tttatggcta attaagattg
    14221 ccaccaccac cagcattaat gccagtgcta atgattcgtt agtaatatta tgcgagttca
    14281 tagcgtgctt cctgcatttc tccggcatgc ggttgcagtg taatcacttt atccgcatga
    14341 ttaatttcgt ctttatcgtg tgtcacccac agcacggcaa tattttgctc gcgcacataa
    14401 cgatggatca tctcattgac gttatgttta ttactttcat ccagcgcact ggttatttca
    14461 tccagcaata aaaccttcgg cataaattgc aggttacgaa tcaatgagat gcgttgtttt
    14521 tcaccaccag atagctcggc gatattcttc gtcaaaatgc tgtccggcaa agcgaagcgt
    14581 tcgagaaaat cgagaaaaat ggccgggtca ggctgctggt cacggatctg ccagggaaag
    14641 atcagattat cgtataccgt atcgccaaac agcgtcggcg tctgggcgca gtaagagact
    14701 tgctggcggt agatttctgg ttttagtgtg ctgacatcct caccttcaaa cagtaacgtt
    14761 ccgctggttg ggctgatcaa tgaagcaact atttttagca gcgtactttt gccacaacca
    14821 gaaggaccgg taattaactt aaattcgcca gcacgcagcg aaaaattgat gttattaaga
    14881 atcttcgtat cacccgccag atatcctacg ttttgtagct gaagcaaagg actgttttcc
    14941 tgcatcgctg ttcccttttt ctgattttta ctaaaaacag tttatccttc gcaggaataa
    15001 gggggaactc tctttcagta atcaggtata atttgtttaa tcaagcagca attttgctga
    15061 ttatggacgg tacaacagga ggtttttccg atgcttatct ttatcccgat tctcattttt
    15121 gtcgcgctgg tcattgtcgg cgcgggcgtc aaaatcgtgc cgcagggcta tcaatggacg
    15181 gtagaacgtt ttggtcgcta taccaaaacg ttacagccgg ggctcagtct ggtggtgccg
    15241 tttatggatc gcattggtcg caagatcaat atgatggagc aagtgctcga tatcccttcc
    15301 caggaagtta tctcgaaaga taacgccaac gttaccatcg acgcagtctg ttttattcag
    15361 gtgattgacg cgccacgcgc tgcctatgaa gtcagcaatc tggagctggc gatcatcaac
    15421 ctgaccatga ctaacatccg taccgtgttg ggttcaatgg aacttgacga aatgctctct
    15481 cagcgcgaca gcatcaactc acgcctgctg cgtattgtcg atgaagccac caacccgtgg
    15541 gggattaaag tcacccgtat tgaaattcgc gacgtgcgcc caccggcaga gcttatctct
    15601 tcaatgaacg cgcagatgaa agcggaacgt accaaacgcg cttacattct tgaagcggaa
    15661 gggatccgtc aggcggaaat cctcaaagcc gaaggtgaaa aacagtcgca aatcctgaaa
    15721 gcggaaggcg aacgtcagtc ggcgttttta caggctgaag cgcgtgaacg ttccgccgaa
    15781 gcagaagccc gcgccaccaa aatggtgtct gaggctatcg cctccggtga tattcaggcg
    15841 gtgaactact tcgtagcgca aaaatacacc gaagcgttac agcagatcgg ttcctccagt
    15901 aacagcaaag tagtgatgat gccattagag gccagcagcc tgatggggtc gattgccggg
    15961 attgccgagc tggtgaaaga cagcgccaac aaacggactc agccatgatg gagttaatgg
    16021 tcgttcatcc acatattttc tggctcagtc tcggcgggtt gctgctggca gccgagatgc
    16081 tgggcggaaa tggttatttg ttgtggagcg gcgtggcagc ggtgattacc ggcctggtgg
    16141 tctggctggt gccgctgggt tgggagtggc aaggggtaat gtttgccatc ctgacgctgc
    16201 tcgccgcctg gctgtggtgg aaatggttgt cgcggcgggt gcgcgaacaa aagcacagcg
    16261 atagtcattt aaaccagcgc gggcagcagc tgattggccg acgttttgtg ctggaatctc
    16321 cgctggtcaa cgggcgcggt catatgcgcg tcggtgacag ttcatggcct gtcagcgcca
    16381 gcgaggatct cggtgcaggt acgcatgttg aagtcattgc gatagaaggg ataaccctac
    16441 atatccgagc cgtctcgtct taatcaccct gcccgatgat gacagcagcc ggagagattt
    16501 tcgataatcg ggcagtcggc gctgtcatcg ccagggcagg cattcgccag tgccagcagc
    16561 tggtcgcgca tggattgcag ctcctcaatg tgtcgttcga tctccgccac cttctccagc
    16621 gtgcgccgtt tgacgtcggc gctgtgccgc tgcgggtcgt taaacagatt caccagctcg
    16681 ccgctctctt ccaggttaaa gcccacctgc cgtgcctggc gcagtaaggt cagttcgttg
    16741 agatgctgct gcgtgtaggt gcgataaccg ttttcgctgc gcatcggcgg cgtcaccagc
    16801 cccttctctt catagaagcg aatggctttg ctggtcaggc cggtaatttt tgctacatcg
    16861 ctgatgttca tcgttcgcgc aacgcctcct gtgctttgtt aaagggtttc aacagatatt
    16921 caagcacgtt tttttgccag tgcgaatatc gaccgccacc acaataatac aggaagtact
    16981 gcgcacagag aggtgttata cggttttatt acgcttcatg accattgcca caataaggct
    17041 gagtaagatt gcaccaataa tccatatcaa cgcacggctg ccctgggacc acaggctgta
    17101 gataaagcca acgatcacca acaacatcac caacgtacca acgataagaa tcggcaacga
    17161 ggcgtgaata tcatgacgca ggcggatcgc caccacaaac accgccagat agcagatcaa
    17221 aaaggtggcg ctggcaacgc tggcgagtga gtctaaattt agcgccgccg tcatcagcat
    17281 tatcaacacc acgacgatga tgttgcccca ggtactctgc cgccacaggg atttattcat
    17341 tagcttcggc agttcgcgtt cgctgcccat gttgtccatg atgttaaaca cggcgaacag
    17401 gttcgcgtta atgcctgaag ccgtcgccag taaagcgccg atgacgacga tcacataacc
    17461 cacatgcccg agcagcggag aagcagcctg cgctacggcg gtatcagcat atttttctaa
    17521 ctctaatgcc gatacatcgc taagcaaaac cagcgccagc gagatataaa gcaacgtggt
    17581 aacgccaatc gccaccagaa acgcccgtgg cataatgacc tgcggatctt tcactttatc
    17641 cgccgcgttc gccatcatgc caaagcccgc ataggcaagg aaggttatcc caatacagga
    17701 gaagaacgca ccggagctgg ggggcgcaga gacggaaata tgcgccggtt gcagcgacca
    17761 gacaccggca ataatcagca ataacaggat catcatttta atgccgacga ggatcacttc
    17821 cagccgccct accgcatggt tgcttaagga gttgaaaagc gtcatcaccg caatgatccc
    17881 caacgcgtag agcaaaataa ggtgctcctc ctggctgcct tcatgcaaaa actgcacggc
    17941 ataagcgcca aaagcacggg caaccatggc gatgctcacc gccagcgtca acaggtatag
    18001 caacgagagc gctagcgaaa agacgccgtt gcctaatccg cgacgaaaaa agtcgataat
    18061 accgccattg cagggatagc tcgcccccag acgcgcatag gcataaccgg aaaacatcgc
    18121 cacaataccg ccaaaagcaa aggcgcccca ggtcgaggct tccattagca acgcagcctg
    18181 ccccagcagc gcgaagatcc ccgcccccac cattgcccca ataccgatgg aaacgacgtt
    18241 ccatagaccg aggggtttgt taccgttatt accttccgtg ttcatcatga tcagccctta
    18301 aacacgttat agccgagttg cttagcgacc gatgccacca ttttttgacc gcgaacacta
    18361 ttgccttctt cgtccagcgg tggtgagaac gcggcaattc ccatcacgcc agggacgacc
    18421 gccagaatac cgccacctac accgcttttg cccggtaaac caacgcgata cgcccagtca
    18481 ccggagcgac catacagccc ttccatcatc atttcggcca gaatgtacgg cacgttgtcg
    18541 gcctgaagaa cgcgtttatg cgtcaacgga ttcacaccac ccgccgccag cgtcgcgcca
    18601 agcgttgcca gttcaacagt attgatgagc gtagagcact gacgggtgta cacgtcacag
    18661 gcttccattg catcacaata gagatatccg gcggagtaca gcagccaggc aatggcccgg
    18721 ttatggaagt tggttgtttg ttccgactgg ttgacttcgt cagagagcgc tacctgctca
    18781 ccagccagtt gctgttggat atgtaaaatt cgctgccagc gttgttcggc gttttcagcg
    18841 ttaatcaggc tggtggtggc aatagcgcca gcatttacca gtggcgaaag cggtttgccg
    18901 ccatgcaact ctaaggcgat aactgagtta aagggcaatc cggtcgggtc agcgccaatt
    18961 ttgtcctgta ccgcctgcgg gccgacatct tctaacgcaa gggctaacgt acagacttta
    19021 gagatggatt ccagtgcaaa gcggtaatca ctgtcacccg cactatagac gttgccatcg
    19081 caggtcacga tagccactgc cgccagttga cctggtacat tcgccagaaa gggaatgtaa
    19141 tcggcatttt gtccgccgtt aagtgagtga aattgggtgt aagcctgatc cactgcctgc
    19201 tgtaatttgt ttgcatctaa catcttttgt taactccttt ttatagatgc gggaggtaat
    19261 tcctcacccc ggtgccgatt ttcaggcatc ctgatttaac ttagcacccg caacttaact
    19321 acaggaaaac aaagaaataa atgtctaatc ctgatgcaaa tcgagccgat tttttaatct
    19381 ttacggactt ttacccgcct ggtttattaa tttcttgacc ttccccttgc tggaaggttt
    19441 aacctttatc acagccagtc aaaactgtct taaaggagtg ttttatgtca caaactatcg
    19501 acctgaacct ggacggcctg tcctgcggtc actgcgttaa acgcgtgaaa gaaagtcttg
    19561 aacagcgccc ggatgtggaa caagcggatg tgtctatcac tgaagcgcac gttaccggga
    19621 ctgccagtgc agaacagctg atcgaaacca tcaaacaagc gggttatgac gcatctgtaa
    19681 gccacccaaa ggctaaaccg ctggcggagt catcaatccc gtcggaagca ctgacagcgg
    19741 tttctgaggc gcttccggca gcgaccgccg atgacgatga cagccagcag ttgctgctga
    19801 gcggcatgag ctgcgccagc tgtgtcaccc gcgtacaaaa tgcgctgcaa agcgtaccgg
    19861 gcgtcactca ggcacgggta aacctagcgg agcgtactgc gctggtgatg ggcagtgcct
    19921 ccccacaaga tttagtgcag gcggtggaaa aagcgggcta cggcgcggaa gcgattgaag
    19981 atgacgcgaa acgccgcgaa cgccagcaag aaaccgccgt cgctacgatg aagcgcttcc
    20041 gctggcaggc aattgtcgca ctggcggtgg gtatcccggt gatggtctgg gggatgatcg
    20101 gcgataacat gatggtcacc gccgacaacc gcagcctgtg gttggttatc ggcctgataa
    20161 ccctggcggt gatggttttc gccggcggcc atttttaccg cagtgcatgg aaaagcctgc
    20221 tgaacggtgc agcgacgatg gatacgctgg tggcgctggg aacgggcgtg gcgtggctct
    20281 attcgatgag cgtcaacctg tggccgcagt ggttcccgat ggaagcgcga catctttact
    20341 atgaagccag tgcgatgatt atcggtctga tcaatctcgg ccatatgctg gaagcacgcg
    20401 cacgtcagcg ttcttctaag gcactggaga aattactcga tttaacccca ccgacggcgc
    20461 gcctggttac tgacgaaggt gaaaaaagcg tgcctctggc agaagtgcag ccaggtatgt
    20521 tgctgcgcct gacgaccggc gatcgcgtgc cggtagatgg cgagattacc cggggcgaag
    20581 catggctgga tgaagcgatg ctgacgggcg aaccaatccc gcagcaaaaa ggcgaaggcg
    20641 atagcgtcca tgccgggaca gtggtacagg acggcagtgt gctgtttcgt gccagtgcgg
    20701 ttggcagcca taccacgcta tcaaggatca ttcgcatggt gcgccaggcc cagagcagca
    20761 agccagaaat cggtcagctg gcggataaaa tctcagccgt atttgtgccg gtagtagtgg
    20821 ttattgcgct tgtcagtgcg gcaatctggt atttctttgg tccggcaccg cagattgtct
    20881 ataccctggt gattgccact acggtgctga ttatcgcctg cccgtgtgcg ctgggactgg
    20941 cgacgccgat gtcgattatt tccggcgtcg ggcgggctgc tgagtttggc gtgctggtgc
    21001 gggacgccga cgcgctgcaa cgcgccagta cactcgatac cgtcgtgttc gataaaaccg
    21061 ggacgctgac cgaagggaag ccgcaggttg tcgcagtgaa aacatttgct gatgttgacg
    21121 aagcgcaggc gctgcgtctg gcggcggcgc tggagcaagg ttccagccat ccattggcac
    21181 gagcgatcct tgataaagca ggcgatatgc agctaccgca ggtcaacggt ttccgcacat
    21241 tgcgcgggct gggcgtgagc ggtgaagcag aaggtcatgc gttattgctg ggtaatcagg
    21301 cgttgttaaa tgatcagcaa gtcgatacta aagcgattga agccgatatt tcagctcagg
    21361 catcgcaagg agcaacgcct gtgctgctgg cgattgacgg gaaagcagta gccctgctgg
    21421 ctgtacgcga tccgttgcgt agtgatagcg tggcggcgct gcaacgcctg cataaagcgg
    21481 gatatcgtct ggtgatgttg accggggata acccaaccac cgccaatgcg atcgccaaag
    21541 aagctggaat tgatgaagtg attgccggag tgctgccgga cggtaaagcc gaagcgatca
    21601 aacgtctgca aagtgaagga cgtcaggtgg caatggtggg cgacggcatt aacgacgcgc
    21661 cagcgctggc ccaggcggat gtcggcattg cgatgggtgg cggcagtgat gttgccattg
    21721 aaaccgcggc gattaccctg atgcgccata gcctgatggg cgttgcggat gcgctcgcga
    21781 tttcccgcgc aacgctgcgc aacatgaagc agaacctgct cggtgccttt atctacaaca
    21841 gcatcggtat tccggtcgcc gccggtattt tgtggccgtt tactggaaca ctgcttaacc
    21901 cggtagttgc cggagcggca atggcgctct cgtcgattac cgtagtgagt aacgccaacc
    21961 ggttgctgcg gtttaaaccg aaggaataag tacgtcattg cggatgcgct gctttagggc
    22021 ggcgcatttg gcatcgacgc atgactgccg gatgcggcgt aaacgcctta tccggcctac
    22081 ggttcaggca caggtttgta ggcctgataa gacgcgtaag cgtcgcatca ggcaatggtg
    22141 ctcggatgcc ggatgcggcg tgaacgcctt atccggccta cgagatgaag cgatgttttg
    22201 gccttacgcg accttttttg cacgctcttc acggcgtgcc aaatattcag ccactgtttc
    22261 aatccgtccc aattcttcct gggtgcgcat gttgttttca acttcccaaa gatcaactgc
    22321 cgcctggagg tttagcctaa aatcgactgt ggtatcaaaa actttcgcca gacggaatgc
    22381 catctcagtg gtgagtttac gattgttatt gatcagtgca ctgacgcgat tacgatgaac
    22441 atgcagcaac tctgctaact cattgatttt caaatcgagc ggttccagat attcatagag
    22501 aagaatatct ccgggtgtcg tcggttttct tgttgcctgt ttcatggttt ttaactgttg
    22561 cttccctgtg aatagcgatg caagaacata ctgaatcata caaacacatt aaacaagtcc
    22621 ccaactccat ttgcgccatc cctcaccgcg cgctagcatg atatttcaca aagggaggtc
    22681 gtatggatct gttgtaccgg gtaaaaacgc tttgggccgc gctgcgcggt aatcattaca
    22741 cctggcctgc catcgatatc accctccccg gcaatcgcca ttttcatctg attggcagta
    22801 ttcatatggg tagccacgat atggctcccc tgcccacccg tttgctcaaa aagctcaaaa
    22861 acgccgatgc gctgatcgtc gaggcggatg tttccaccag cgatacgcct tttgctaatt
    22921 tgcctgcctg cgaggcactg gaagagcgca ttagcgagga gcaactccaa aacctgcagc
    22981 acattagcca ggagatgggc atttctccct cactcttttc tacccaaccg ctgtggcaaa
    23041 tcgcgatggt tcttcaggcg acgcaggcac aaaaactggg gctgcgggca gaatacggta
    23101 tcgattacca gctattgcag gcggcgaagc aacaacataa acccgtgatt gaactggaag
    23161 gggctgaaaa ccagattgcc atgttgctcc agctccctga caaaggactg gcgctgctgg
    23221 acgatacgct gacccactgg cataccaacg cacggttgct gcaacaaatg atgagctggt
    23281 ggctgaatgc accgccgcaa aataatgata taacgctgcc caatacgttc agtcagtcgc
    23341 tgtacgatgt gctgatgcat cagcgaaatc tcgcctggcg ggataaatta cgcgccatgc
    23401 cgccggggcg atatgtggtc gcggtcggtg cactacacct gtatggagaa gggaatttgc
    23461 cgcaaatgtt gcgctaaaaa aatggccaat atcgctattg gcccgtcaaa gaggaatttc
    23521 atttttttat tattatgccg tcactttaag cgacggtgta actcgattgt cgctcaatct
    23581 caccatcctg ccaatactct taggcaagat gactcttttt tttggaccgc catcaggcgt
    23641 atgcttgctt cgtttttttg tttaaggcag gaaaattacc atgacacccg cagttaaatt
    23701 actcgaaaaa aacaagattt cgtttcaaat ccatacctac gagcacgatc cggctgaaac
    23761 caattttggc gatgaagtcg tcaaaaaatt aggtttgaat ccggatcagg tctacaaaac
    23821 gctgctggtg gcagtgaacg gtgatatgaa acaccttgcc gtggccgtta cgccggtcgc
    23881 aggtcaactg gatcttaaaa aagtagcaaa agcgctgggt gccaagaaag ttgagatggc
    23941 cgatccgatg gtcgcgcagc gttcgacggg atacctggtt ggggggatta gcccactggg
    24001 gcagaaaaaa cgtctgccaa cgattatcga cgcccccgca caagaatttg ccactattta
    24061 tgtttccggc ggcaagcgcg gactggatat cgaactggcg gcaggcgatc tggcaaagat
    24121 cctcgatgcc aaatttgctg atatcgcccg ccgcgattaa gcattgtgcc ggatgcaaac
    24181 atccggcact ttcggattac tgccagctca cctcaccttt cggttcataa acactcacat
    24241 ccagcggcga gcttttctgg atatacgctt tcagcacttc ggcatcaata aagccggtat
    24301 tcacatagcc cggattgtta tcaaggcgcg gatatccatc accgccggtg gcattgaagt
    24361 ttaatgtcgc catacggtaa gttttcgccg gatcgaccgg ttcgcctttg attttaaggt
    24421 cgttcagttt gccgtctttc gccacaaagc taacgttggc aaattgcggg taggcacctg
    24481 aatctggctt catctgcgcg acggcggtca ggtaatcaat cacctcttta ccggtcatgt
    24541 cggcatacac caccacattg ccgaatggct gcactttcag cacgttttta tagctgatat
    24601 cgcctgcttc gatagaatca cgaattccgc ctccgctcat caccgcaaag tcggcaccag
    24661 tgcgatccat ttgggctgcc agaatcaacc gccccatatt ggtctgtaca aaacgcactt
    24721 tgtcacgatc gccttccaga cgaccattgg tttcgcctat tttcacttcc agctgcgctt
    24781 tgcctttgtt ctggaacggt gataacagcg agatcatttg ctggttttca gcgatttcag
    24841 gagtgtaaag cacgcgctcg cttttcccgt cttcccaggt cactttcttc ttcaggttca
    24901 ccggaatcag ctggtagtta accattttca tttcgccatt acgaaactca aaatcagccc
    24961 gtcccacgta tttgccccac tcatgcgcct gcacaatcca gatgccgttt tgttgatctg
    25021 gtttgcatgg cgtacccggc acgtaatcga cctgtttttt gttttctgcc gccatgcaga
    25081 ccggatcttg cgagtgacca ccgacgatca tcgccagcga tccggcaggc agcgcgcgtg
    25141 ccatctccac atcgcccggt gcgttagagc cgtactcacc attatcgtaa tgccccatat
    25201 gggtcgccgc gataataatg tctggctttt ctgtctgttg cagctcctga atcaccagct
    25261 tcgcttcatc ggcgggctta cgaaattcga tatcagtgaa gtattccggg ttaccaattt
    25321 ttgctgtgtc atcggttgtc agcccaataa cggcaatttt cagatcctga cgcttaaaca
    25381 gcgcccacgg tttaaacagg cgctcgccag tacttttctg gtagatattc gcggaaagca
    25441 acgggaactt ggcccacttt tcctgctgac gtaatacggt gagcggatta tcaaattcat
    25501 gattaccgat cgccatcgcg tcatagccca ccagattcat accgcgaaaa tcaggttcgg
    25561 catcctgtaa gtcagactcg ggcacgccag tgttaatgtc gccaccggaa agtagcagca
    25621 cgctaccgcc ttcagccgca acctctttgc ggataccatc caccagcgtt ttttgcgccg
    25681 ccagaccata ttcgccatat tcattgcgcc aaaaatgccc atgatgatca ttggtatgca
    25741 gaactgtaat tttgtaggtt ttatcctgct catacgccag agcagtttca ctcgccagtg
    25801 taaatgtggt taacagcgct aacgccacgc cccgctgcaa taatttcata cttctctccc
    25861 tgacctgatt tcaacgtgtt gaaaaatagt atgacgcgac atcatagaca agtctgcgtc
    25921 cggtgaagat aaatctgcat ccagattaga aattctgtat tcggcgttga gacttccttc
    25981 agacgacgca tacaggtatg ttagtcgggt aataatcaca aataagatca ttctttacat
    26041 cgcaaccctg aaaaagtgac tttcctatgg caatgagtga acaaacccag cctgtggcgg
    26101 gcgcggctgc gtcaacgacc aaggcccgaa catcgtttgg tattttaggt gctatcagcc
    26161 tctcacatct gctgaacgac atgatccaat cgctgattct ggcgatttat ccgctgcttc
    26221 agtcagaatt ttctctgaca tttatgcaga ttggcatgat aaccctcacc ttccagctcg
    26281 cctcttcgct actgcaacca gtggtcggct actggaccga taaatatccg atgccgtggt
    26341 cgttgccaat tggcatgtgc tttaccttaa gtggtctggt gctgcttgcg ctggcgggca
    26401 gttttggcgc agttctgctg gcggcggcgc tggtcggtac cggttcatcg gtctttcatc
    26461 cggaatcttc tcgcgtagcc cgtatggctt ccggcgggcg gcatggccta gcgcaatcta
    26521 tctttcaggt cggcggcaac tttggcagtt ccctgggacc cttgctggcg gcggtgatta
    26581 tcgcgcctta tggcaaaggc aacgttgcct ggtttgtgct tgcggcactg ctggcgatcg
    26641 tggtgttggc gcaaattagc cgttggtact cggcacagca ccgaatgaat aaaggaaaac
    26701 ccaaagcgac gataatcaat ccactgccgc gcaacaaagt ggtactggca gtcagcattc
    26761 tgttaatcct cattttctcg aaatatttct atatggcgag catcagcagc tattacacct
    26821 tttatctgat gcaaaaattc ggattatcta tccagaatgc ccagcttcat ctgtttgcct
    26881 tcctgtttgc cgttgcggca ggtacggtga tcggcgggcc tgtaggggat aaaattggac
    26941 ggaaatatgt gatttggggc tctatcctcg gcgttgcgcc gtttacgctg attttaccct
    27001 acgcccgcct gcactggacg ggggttttaa cggtgattat tggatttatc ctcgcttcgg
    27061 cattctctgc cattctggtc tacgctcagg agctacttcc gggacgtatc ggtatggttt
    27121 ctggactctt tttcggtttt gctttcggca tgggaggtct gggagcggca gttctggggc
    27181 ttatcgccga tcacaccagc atcgagttag tctataaaat ctgtgctttc ctgccactat
    27241 tggggatgtt gaccatattc ctgcctgata accggcataa agactgattt cattgcagcc
    27301 aaaggcaaac tttggctgca tcgttttaca gtcgccataa gcctttcctc tgttaaaccg
    27361 ccttctgttt tccgatccgt gctttttttg cagctaattc catttttctg caaaattcaa
    27421 caattattca taaacatcat caatgaaatt gtcataaact attacctgta attttggttt
    27481 tcccggccaa aaatggacaa taaccaccag gaaaaggaga cggaatgcat cacgccaccc
    27541 cgcttatcac caccattgtt ggcggccttg tgctcgcctt tatcctcggc atgctggcca
    27601 ataaactacg tatttctcct ctggtgggat atctgttagc gggtgtgctg gcaggaccat
    27661 tcactccggg ctttgttgcc gataccaagc ttgcgccgga actggctgaa ctgggcgtta
    27721 ttttgctgat gttcggcgtc ggtttgcatt tttcgctcaa ggatttgatg gcggtaaagt
    27781 ccatcgccat tcccggcgcg atcgcccaga tagccgtggc gacgctgctg ggtatggcgc
    27841 tctccgccgt gctgggctgg tcgttaatga ccggtatcgt gttcggctta tgtctttcca
    27901 ccgccagtac cgtggtgtta ctgcgcgcac ttgaagaacg gcaattaata gacagtcagc
    27961 gtgggcaaat cgccatcggt tggttgattg tggaagacct ggtgatggtt ctgacgctgg
    28021 tgttgctgcc cgcagtggca ggaatgatgg aacagggcga tgtgggcttt gccactcttg
    28081 cagtcgatat ggggatcacc atcggcaagg tgatcgcatt tatcgccatt atgatgctgg
    28141 taggtcgccg tctggtgccg tggattatgg cacgcagcgc ggcaaccggt tctcgcgagc
    28201 tgtttaccct gtcagtgctg gcgctggcgt tagggattgc ctttggtgcg gtagagctgt
    28261 ttgatgtctc ctttgcactc ggtgcgttct ttgccgggat ggtactgaac gagtctgaat
    28321 tgagtcaccg tgccgcccac gatacgttgc cattgcgcga cgcgtttgcg gtgctgtttt
    28381 ttgtctcagt cgggatgttg tttgatccgt taattctgat tcagcaaccg ctggcagtgc
    28441 tggcgacgct ggcaattatt ctgtttggta aatcggtcgc ggccctgttc cttgtccgtc
    28501 tgtttggtca ctcccaacgt acggcattaa ccatcgccgc cagcctggcg cagattggtg
    28561 agttcgcgtt tatcctggcg ggactgggaa tggcattgaa tttactgccg caggccggac
    28621 aaaacctggt actggcaggg gcgatcctgt cgattatgct caacccggta ctgttcgcac
    28681 tactggagaa atatctggcg aagaccgaaa cgctggaaga gcagacgctg gaagaggcaa
    28741 tcgaagaaga gaaacagatc ccagtggata tttgcaacca tgcgctattg gtgggttacg
    28801 gtcgtgtagg cagcctgctg ggggagaaat tgctcgcctc tgatattccg ctggtggtga
    28861 ttgagacgtc acgaacccgt gttgatgagc tgcgagagcg tggggtccgc gctgtattgg
    28921 gcaatgcagc gaacgaagaa attatgcaac tggcacatct ggaatgtgca aaatggctga
    28981 tcctgacgat ccccaacggt tatgaagcgg gtgagattgt ggcgtctgcc cgcgcgaaaa
    29041 acccggatat tgagattatc gcccgcgccc attatgacga tgaagtgacg tatatcaccg
    29101 aacgtggcgc gaatcaggta gtgatgggtg aacgtgaaat tgcccgtact atgctggaac
    29161 tgctggaaac gccaccggcg ggtgaagtgg tgacggggta aacggtggtt gccggatgta
    29221 aaatatgaaa aatatgtagg catgataaga cgcgccagcg tcgcatcagg catcgagagc
    29281 caaatgccag atgcggcgtg aacgccttat ctggcctacg aaccgacgat aacttaacga
    29341 tcccagtaag actcttccag gctgtcttca cgctccggca agccgcgcgt taaacgaggt
    29401 gaatgctggt tcagtacctg atagctcaca cggttagcat atttacacac ctgcgctaac
    29461 gatgaataag ttaaccaggt gaatttatgt ttgctggagt ttggtatgtt gctacgatgg
    29521 tagctgttgg cggtaatgtc atgcagcaac gctgccaatg cgccatcccc cgctccatta
    29581 gtgttcatga ttttttccgg cccgcccatg tacggcgcaa tgtgcgaata gacacgcagc
    29641 ggattctggc aatccttgtg gcgcatggcg cggctaaact catactggtt gaattccgct
    29701 atagcgcccg gcagcagcgg atgctgggtt ttacgtttcg cttcgtcttc ggtaaagccc
    29761 gccatataca agccgattgg cccggcggtg cacagcacca gatctaccca gtccagcgcc
    29821 ttgtcagatg ccaacaacgg atcgctttct ccggtcaacg cttcggcttc atcttcgttc
    29881 atcgcaagga tagaaacgtg gtctttgagg aattgctgcc accactgcgg attctcggca
    29941 atgacaaact tggtgcccag cgtcagcacc accggtacgt tatatttctt cgcgtactca
    30001 atggctttca tggttgcttc cggcatgggt tcacccggct tgcaacgcac cagatatgag
    30061 gtgagaacca gtgccgaggc tccggcaatc acatcttccg gaatgctttc agcccgcagc
    30121 tggttcatgt ggcttggact gatagcaaag gtacgttccc cggactcgcc aatcagcgta
    30181 aagcaacgac caatcgggcc atccacgcct tgtagatagt taagatcggt acggctggaa
    30241 gtgttacaca ggtaacgata ggcataactg ccaatttcaa tattgctgca catgacgccc
    30301 agcagcaccg aacggtcgtc cgcgagcacc gagtagttgt gcatggtgtt accaatggtg
    30361 ccacccgcaa actgatgggt aatcagattt ttctgtttta gctcctgata aagcgcttcg
    30421 gctacgtcat cctcaatcac cagtgaatgc ccggcgctta atccataacg ctcaatgaat
    30481 tcatcatcca ctttcgcttc aatatcgacc agcgtctggt caatacctac gacccaggcg
    30541 gcgctggttt cgttttctgg ctggaattgc tgaagcagcg gatcgcgtgc atttacaggg
    30601 aagtaatgtt tggatttacg tttaccggga aatttcatgg ttgtttttac gggtggttaa
    30661 ctgagcggcg aatgttaaca caatctgacg ggagcgcgcg agacgcagcc agtttcaata
    30721 tcagcaggcg ggatcgcatt ttcatggcag tggttcctta caatgacgta atttgaaagg
    30781 agtttttgta tgaagccgga aaacaaacta cctgttctgg accttatttc tgctgaaatg
    30841 aagaccgttg tgaatactct tcagccggat ttaccgtcct ggcccgcaac gggaacgatt
    30901 gctgagcaac gacagtatta cacgcttgag cgccgattct ggaatgcggg cgctccagaa
    30961 atggcaacca gagcttacat ggttccaaca aaatatgggc aggtggaaac acgtctcttt
    31021 tgtccgcagc cagatagccc ggcgacgcta ttttatttgc atggaggcgg ttttattctc
    31081 ggcaatctcg atacccacga tcgcatcatg cgcctgctgg caagctacag ccaatgtacg
    31141 gtgattggta ttgattacac cctttcacct gaagcgcgtt ttccacaagc gatagaggaa
    31201 attgtggctg cttgttgtta tttccaccag caggcggagg attatcaaat caatatgtcc
    31261 cgcattggct ttgccggtga ttccgcaggt gccatgctgg cgctcgccag tgcgttgtgg
    31321 ttgcgtgata aacagatcga ttgcggtaaa attgcgggtg ttttgctgtg gtatgggctt
    31381 tacggattac gggattccgt gactcgtcgt ctgttgggcg gtgtctggga tggcttaacg
    31441 caacaggatt tgcagatgta tgaagaggcg tatttaagca acgacgcgga ccgcgagtca
    31501 ccgtattact gtctgtttaa taatgatctc actcgcgaag ttccgccctg ttttattgcc
    31561 ggggcggagt tcgatccgct gctggatgac agccgtctgc tttaccagac gttagcggcg
    31621 catcagcagc cctgtgagtt caaactctac ccaggtacgc tgcatgcctt tttgcattat
    31681 tcacggatga tgaaaaccgc cgacgaggct cttcgcgacg gcgctcagtt ctttaccgct
    31741 cagctttagc gatacgcggc aacaagatta gccatcattt cgatatgttc cggcgtggca
    31801 ttaagcgctg gaatatattc atattttttc ccgcccgcac cgaggaagac ctcacggttt
    31861 tgctcggcaa tctcttccag cgtctccaga caatccgcag caaagcccgg gcacatcacc
    31921 tgtatatgac ctacgccttt ttctccgagc attttcagcg cttcgtcggt ataaggcatc
    31981 agccagggtt cccggccaaa gcgcgactga aaggtcatca tcactttttc cggtgccatc
    32041 cccagtgcgg aagccagttc gcgagtcgtt gtgcggcaac gttgcgggta atcatcgcct
    32101 tcatctgcat aacgctgggg aatgccatga taagagagca gcagcagatc tggttcgcca
    32161 tgtttggcaa aagaagcgcg tacgctgttc gccagtgcat taatgtaatc gtggttatcg
    32221 gcgtaatcac gaataaacga tatccccgga atgctacgtt tgcgcgccag aatgcgtgcc
    32281 agttcatccc ataccgcacc gaccgtagaa caggagaatt gcggataaag cggcagcacc
    32341 acaatatgat ctacatgctc tgccaggagt tcatcgacgg cgctttccag tgatggcgag
    32401 ccgtagctca tgcccagcgc cactggcgta tccggtaaac gttgtgccaa cgcctgctgt
    32461 tgctggcggc tgtaaaccat cagcggcgag ccgtcttcca tccagacaga ggcgtacagc
    32521 ttcgccacac gcggcgagcg cagcggcaaa atcacgccgc gcagcaacgg ccaccataac
    32581 aaccgtgagg tatcaaccac gcgtctgtcg cttaaaaatt gtttcagata gcgttttacc
    32641 gcttcaggtg tgggggcatc gggcgtaccc aggtttgcca gcaggatacc ggttttagtc
    32701 tgacgcatta ccgcctctta tcgattcaac ttgttgataa ttgtagcgga aaagtgagga
    32761 agaagaacta attgctgtga gaggtattgc ctgaggtgga tgagcagacc tgcccgaaag
    32821 cagatctgct gcggcttgat tagccgagga ttttttccag atcagcgcga acttcagcaa
    32881 ccggcttggt accgtcaact ttcgcgtatt tggtgttacc cgcttccgct tctttggagt
    32941 agtagccgat cagcggtgca gtcatctgat ggtattcaac cagacgttta cgcacggttt
    33001 cttcctgatc gtctttacgg gtagtcagtt cttcaccggt aacgtcgtct ttgccttcta
    33061 ctttcggcgg attgaattta acgtgataaa cacgaccaga cggcgcatga acgcggcggc
    33121 ctacgatacg atcaacaatc agttcgtccg gtacgtcgaa ttccagaacg taatcaacat
    33181 tgatgcccgc ttctttcatc gcgtctgcct gcggaatggt acgcgggaag ccgtccaaca
    33241 ggaaaccatt acggcagtct tcctgagcaa tgcgctcttt aaccagcgcg atcaccagtt
    33301 cgtcggtgac cagtttgcca gcatccataa tgtcttttgc ttgtttaccc agctcggagc
    33361 cagatttgac cgcagcacgc agcatatcgc cagtggagat ttgcggaata ccatatttct
    33421 ccatgatgaa ctgagcctga gtccctttcc ccgcgcccgg agcgccaagc agaatgatac
    33481 gcattgcgaa aatcccctta aaatgtgtcg atttttttga aaaagcgata aacgatacca
    33541 ccaacaggcg attgcctcaa gaaaggcacc tgaggctgaa acggttaatg gcgagataat
    33601 ttgcggaaag tgcagatgat gaaaagaaaa atgccggatg acacaaaggt catccggcat
    33661 gacatcagga aaccagcagc tggttcatac gacgaataaa caggttcgga tcttccagcg
    33721 tgccgcgttc tgccagcagc gcctgatcca gcagcagttc tacccactcg ctgaacttgg
    33781 cttcatcttc agtatctgcc gcacgtttca ccagtacgtg atccgggttc agttcgaaga
    33841 tgtatttcac ttctggcact ttctggcccg ccgcagcgaa cagtttcgcc atctgagtgc
    33901 tcatttcgtc cgcgtcggtc gaaacgatcg ctggcgtatc ggtcagacgg tgagtcagac
    33961 ggacatcttt cacgcgctcg ccgagcagag ctttcacgcg gtcgatgaac ggagtcagtg
    34021 ctttctccgc ttctttcgcg ctctcatcaa cttcgtcagc cagtttttcc agcgactcgt
    34081 caactttaga caccgactgg aacggtttac cgtcgaactc agtcagatag ttcatcatcc
    34141 actcatcgat gcggtcggaa agcagcagaa cttcgatgcc tttcttacgc agcagttcca
    34201 ggtgcgggct gctcttcgct gccgcatagc tgtctgcagt gatgtagtag attttctcct
    34261 gcccttcttt catgcgggaa acgtagtctt ccagagacac ggtctgcgca gaagaatcgg
    34321 tataagtaga agcaaaacgc agcagtttgg cgatcgcttc ctggttagcg aaatcttccg
    34381 ccggaccttc tttcagtacc aggccaaact gttgccagaa ggtctggtat ttttccgcgt
    34441 cgtctttcgc cagtttttcc agcatttgca gcacacgctt ggtcagcgca ttgcgcaggt
    34501 tacgcgttac cgtgctgtcc tggaggattt cacgggaaac gttcagcggc agatcgctgg
    34561 agtcaatcag accacgcacg aagcgcagat agttcggcat gaactgttca gcgtcgtcca
    34621 tgataaacac gcgttgaacg tacagtttca ggccgtgttt atgatcacgg ttccacatat
    34681 cccacggagc ctgggacggg atgtacagca ggctggtgta ctcctgctta ccttcaacgc
    34741 ggttgtggct ccaggtcagc ggatcgttaa agtcgtgggc gatgtgtttg tagaactctt
    34801 tatactcttc atcggtgatt tccgacttgt tacgagtcca cagcgcctgc gctttgttga
    34861 tttttgccca ggagataacg gtttcgccat ctttctcttc gcgtttttcg atctctaccg
    34921 gcagcgcgat atggtcggag tatttgctga tgatggaacg cacgcgccag tcatcgagga
    34981 actcgtcttc gccttcacgc agatgcaggg tgatttcagt accacgatct tctttggtga
    35041 tgtcggcaac ggtatattca ccttcgccag ccgattccca gaagacgccg ttttctggtt
    35101 tttcgcctgc cgcgcgagta cgcacggtca ctttgtcggc cacgataaac gcagagtaga
    35161 aaccaacacc aaactgaccg atcagctggc tgtctttcgc ctggtcagaa cccagggatt
    35221 cgaggaatga tttggtaccg gatttagcga tagtccccag atggtcaatc acttcgtcgc
    35281 gggtcatccc cacgccgtta tcggagatgg tcagcgtgcg cttgtcttta tcgaaagaga
    35341 cacgaacgcg cagttcgcca tcaccttcgt acaggtccgg gttagatagc gcacggaaac
    35401 gcagcttgtc cgccgcatcg gaggcgttag agataagctc acgcaggaag atttctttat
    35461 tggaatagag agaatggatc atcaggtgca gaagctgttt cacttctgac tgaaaaccac
    35521 gagtttcttg tcctttcatg taggtctacc tcaataatgc cattttaagg ttaaaaacag
    35581 gatgagggag agatggggac aagggagaat aatttcaagc gagagcaggt gctcctgctc
    35641 tcgtttgctt aaaaacgaat cttatgacgc ccggcgaggg agtgtgacag cgtggtgcca
    35701 tcgaccattt ccagttcgcc gccaaccggt acgccatgag cgattcggct ggcttccacg
    35761 tcatattgcg cgcaaagctc ggcaatgtag ttagcggtag cttcaccttc aaccgtggga
    35821 ttggtggcga ggatcacttc agtgattttt tcctctgcca gacgctgttc cagacgatca
    35881 agcccgatat catccggacc gatgccgtcc agcggtgaca gatgccccat caacacaaaa
    35941 taacgaccag aaaactgccc cgtctgctca atggcgtaga tgtccgccgg actctccacc
    36001 acacagattt gaccgttttc ctgacgacgc ggattcgaac agatgttaca gacttcctgt
    36061 tcggtgaaag tgcggcaatc ggcgcagtgg ccgatttccg acatcgcccg ggtgagcgcc
    36121 tgcgccagac gcatcccgcc gctacgatcg cgctgaagca gcgtgaacgc catacgctgc
    36181 gccgacttcg ggccaacgcc cggcagacag cgcagtgctt ccataagctg tgttaacagc
    36241 gggctggttt gcatcagaac ggcatcttaa agccaggcgg cagctgcatt ccggaggata
    36301 cagaggccat tttttctttc tgcgtttctt caatacgacg tgctgcgtcg ttgaatgctg
    36361 cagccaccaa gtcttccagc atctctttgt cgtcttccag caggctcggg tcgatctcta
    36421 cgcgacggca gttgtgtgca ccgttgatgt tcacttttac cagacctgcg ccagattcgc
    36481 cggtaacttc cagctgcgcg atctcttcct gcattttctg cattttttct tgcatctgct
    36541 gggcttgctt catcaggtta cccagaccgc ctttaccaaa cataggtttc tctctcaatc
    36601 acgttaagga tgacgaacgt aagctgtgct tacgatcaaa tggggcggat actttcttca
    36661 tccagctccg catcgaaaaa ccgacgcagg gtctgaatat tattatccgc aataatggac
    36721 tcgcgcgcct gcgcaagttt ttcttcgtat atcgcctgac gccactccag cggcgtacgc
    36781 accgcgggat tatcatcttc aacgatagtc agttcaaccg ttgaaccttt taacatgctc
    36841 aacgcttcag ccagtttttg ctgtgcaccg cggttgttca aatgccgctg agaggagcgc
    36901 aaatgcagac atactgcgtt gtcgctctcc tctttccagg catttaacgc cacctgttcg
    36961 accagttttg gtagcgaaag ttggctcacc tgtgccgccc acgggtcgcg ctcaatggct
    37021 tctgccgcta gcttcgccgc cagttccggc gttttttcat gttccagcgc ttttttcagc
    37081 gccttcggcg tggcgaccac ttctttttgc tgcatcaccg gagtggtcgc cttccagcga
    37141 tacgcttctt ttttggctgg cgctttttcc agcgccgatg gcaccggacg cgcctgaacg
    37201 cgatcggtga ccgaagccag tctttccagc gcagcgttat tcaccggccg cgcgcgggta
    37261 gcggctgccg gttcactctt ttttgctttg gttgctccct gcacgcgctg caactgctgg
    37321 cgcgccgcca gcacctggct ggtggtttcc gggagcggta cagtcggtgc ctgctgcggc
    37381 gctgattgcg gcggcaccag ggttggcgtc attactgccg ttggcgcgac gggtgcaaag
    37441 gactggcgtg gcacttctgg ctcaggcagc ggcatacgcg gatggaacgc cagcgcgcgc
    37501 agcagcgtca tctcaacgcc catgcggcgg tccggcgcat acggtaattc tttgcgacca
    37561 atcaacagcg tctgatagta aagctgaata tccgtcggcg gtatggtgcg cgccagttca
    37621 cgcatccgca gctcgatggc ggccatgtcg ttgccaagtg cagcaggcga aagttgtacc
    37681 atcgcaatac gatgcaacaa gccgagcatt tccaccagca acgcttccca ctcgatacca
    37741 cgggcagcgg cttcattaat cagtgccatt acgcgctcgc cgttgccctc gaccatcgct
    37801 tcaaccagcg acagcgcctg atcgtcgtca agcgtaccca gcatcgcact gaccgcctgg
    37861 gttgaaacct ggccgtcacc gctggcaatc gcctggtcgg tcagacttaa ggcatctcgc
    37921 aggctgcctt cagcggcgcg cgccaacaat tgcagcgccc gcggctcgtg agcgatatgt
    37981 tcttcattga ggatgtgctc aagctgatgg cgaatttgct cgacatccag cgccttgaga
    38041 tgaaattgca gacagcgtga caaaatcgtc accggcaatt tctgtggatc ggtcgtcgcc
    38101 agcagaaact taacgtgctc cggcggctct tcaagggttt ttaacagtgc gttaaagctg
    38161 tggcgcgaca gcatatgcac ttcgtcgatc agataaactt tgaaacgacc acgcgccgga
    38221 gcgtactgga cgttatccag caggtcgcgg gtatcttcaa ctttggtgcg cgaggcggcg
    38281 tcgatttcaa tcagatcgac aaagcgcccc tgctcgattt cacgacagtt atcgcacacg
    38341 ccgcacggcg tcgcggtaat gccggtttcg cagtttagcc ccttcgccag cagtcgggcg
    38401 atagaggttt ttccgacgcc acgggtgccg gaaaaaagat aagcatgatg aatacgccct
    38461 aacgacaagc cgttcgccag tgcggtcagc acatgttcct ggccgacgac gtcagcaaag
    38521 gtttgtgggc gccattttcg ggctaaaacc tgataactca ttggcaggct ctgaaacgct
    38581 ggaaggtgga ttcacgaagg ggtaatgcta acacagcccc gtcagaacgg cgaggctgaa
    38641 tgcacatctt gtgctgtccg tagcgtgggc agcacaagac tggcgataat taatggcccg
    38701 ggaacgggac aaggctgtag ctggtaatgc cctgtttttc gagacgctgt tcgccgccga
    38761 gatcgaacag gttgataatg aacgcagcgt cagccacttc accacccaga cgacggatca
    38821 gtttaacggt cgcttcgata gtgccgccgg ttgccagcag atcgtccacc accagaactt
    38881 tgtcgcccgg tttgatggca tcaacgtgga tttccagctg atcggtgccg tattccaggt
    38941 cgtaattttc actgatggtt tcacgcggca gtttgcccgg tttacggacc ggtacaaagc
    39001 caacgcccag acccagagct accggagcgc caaacaagaa gccacgcgct tcggtgccga
    39061 caactttggt aatgcccgca tttttgtaac gctcaaccag caagtcgatg ctgagagcgt
    39121 aagctttcgg gtcttccagt aagctggtga catcgcggaa aagaatgccg ggttttgggt
    39181 agtcctgaat gcttttgatg ctatttttga gatactcaag ctgctgtgca gtcgcggtca
    39241 taagtgtatg cctgcttgtt acggtggtac tcacggcgcg ttttaaacgt atcaaaagta
    39301 actgttgttt aacctttgac cgcctgcgcc tgtgctcgaa aacgccagaa tgtactggct
    39361 gtacgcaata attgcaactg cgattgttgt gcttcagtgc ttttcttgct tttcatcaat
    39421 caccggaatt cgccacatat agaaaagcaa acaggcgaga attaccagca acatgatgcg
    39481 cacccatggc atctggacga accacagaga aatggcaaac gtgagcaaaa taagcaaaat
    39541 cgcccgcggt ttgacgccgc gcggcatcgc atgatgtttc tgccagaaac gtagatagct
    39601 gccaaaccat gagcggtaca gcaaccaggc gtgaaagcgc ggggaagaac gggcaaagca
    39661 ccaggccgcc agcaggataa acggcgtcgt cggtaatacc ggtaatacca cgcccagcgt
    39721 acccagcact accgccagcc agccaatgat gattaaaatg attcgttgca tattgagtgt
    39781 tgtcagctta cagagtggct actttagcat aacaattatc attttcattg aggtcttatc
    39841 gtgaaaaccg ccctgctgct ggaaaaactg gaaggacagc tcgctacgct gcgtcagcgt
    39901 tgtgccccgg tgtcacagtt cgccacgcta agtgctcgtt tcgacaggca tctttttcag
    39961 actcgtgcga caacactaca ggcttgtctc gacgaggcgg gcgataatct ggctgcgctt
    40021 cgtcatgcag ttgagcagca acagctgccg caagtggcct ggctggcgga acatctggcg
    40081 gcacaactgg aagccatcgc gcgtgaagcc tccgcctggt cattgcgcga gtgggacagt
    40141 gcgccaccga aaattgcccg ctggcagcgt aaacgtattc agcatcagga ttttgagcgg
    40201 cggctacgtg agatggttgc cgaacgcaga gcccgcctgg cgcgggtgac cgatctcgtg
    40261 gaacagcaaa cgctgcatcg tgaagtggaa gcctatgaag cgcgtctggc acgctgccgc
    40321 catgcgctgg aaaaaatcga aaacaggcta gcgcgcttaa cccgctagca atggagagag
    40381 tatgtcactt gaaaatgccc ctgacgatgt caaactggct gtcgatttga ttgtgctgct
    40441 ggaggaaaat cagatccctg ccagtaccgt gctgcgcgcg ctggatattg taaagcgtga
    40501 ttatgaaaag aaattaacgc acgatgatga ggcggaaaag tgagaaataa atggggccgc
    40561 taaatgtccc ctaagattgc gggcaaacgc aaaactgcct gatgcgctac gcttatcagg
    40621 cctacaccgc tcctgcaata tattgaattc ttgcggtttc gtaggccgga taaggcggtc
    40681 acgccgcatc cggcatgaac aacgcgcact ttgtcagtaa tctcggggca actaaatgtc
    40741 gccccctttg gttattaccc aaccgctggt gtcggatcgt cgcctttgta gtcgcgtttt
    40801 acttccgtca cctcatcgcc cttctcgttg tgcagatgca cttcaagctg gttaaaggca
    40861 atgttgatgt cgttttcacg gcacagctga tcgatagtac ggttcagctc atcgacagta
    40921 cggctacggt cacgcagttc acgcacatac agacgcagct catgatccaa cgtgctggca
    40981 ccaaatgccg taaagaagac ttccggcatt ggttcgtgca tcacccttgg gtgctcagtc
    41041 gccgccttca gtaacacttt acgcactttt tccagatcgg agccataggc cacgccgaga
    41101 cggatcacca gacgcgtagt agtgtcagtc aacgaccagt tgatcagacg ctcggtaaca
    41161 aacgctttgt tcgggatgat cacttctttg cgatcgaaat cggtaatcgt tgtcgcacga
    41221 atacggatct tacttaccgt ccccgagaag ctaccaatgg ttaccgtatc gccaatacgc
    41281 accggacgtt cgaacagaat gatcaaaccg gagacgaagt taccgaaaat ttcttgtaaa
    41341 ccaaaaccaa gacctacgga taatgctgcg gccagccact ggagtttatc ccaagagacg
    41401 cccagcgatc cgaacaccgt catcgcacca acggcaataa tgatgtagtt aaggatggta
    41461 gtaatggcat acgacgcgcc ctggcgcata ttcagtcgcg agagcaccag cacttccagt
    41521 aaaccaggca ggttgcgaat caacgcccag gccaccattg aggcgataat cgcaaacaac
    41581 agactgccca tggtgacgtt tttcaccacc gcagcgccag cttcagtgcc gttgtaatgc
    41641 cagagcgtga tgctgtcgag atagctgaac acggtgatca aatcggacca aattgcccag
    41701 aacatgacac cgaacagcgc aaacatcagc aacatggtaa tacgcagcgt ctgctggtta
    41761 acttgctcca gtgcaatggt gggttcttcc ggcggttcgg caccttctgc gccctctttc
    41821 accagattct gccgacgcgc cagcgcacga cgccaggcga tacgccgcgc cgctacgctt
    41881 aagccgcgca ataccgtctg gtagagcaag ttccagatga tcaccagata aacggtttca
    41941 atccaacgcc cggagaggcg cagcgtggtg tagaaataac ccgtggcagt cagcaccatc
    42001 aatgcaatcg ggataatcga cagcacggta atggtgacca gtcgcatggt gtgcgactct
    42061 ttatcacgcc agctttcgcg gcacattggc cataccagga aggcaatcag cagcaggttg
    42121 aagaaaatca tcgcctgccc cagcacatca tccatcagat gcagcgggga aagttctgcc
    42181 accacagacc agaaatggat aggcagcaac gcgagactga tgcggacaat ttgccgacgc
    42241 cagtggctgg tctgctgctc cggcatgccg aagtgacgca cggcaacgcc atttttctcc
    42301 agtaccttcc agcacaggcc aaacaccagc cagaaaatcg ccagtttttt actgaacgac
    42361 catagcagtt cgctgatgtt gagctgcatg gtcaacagaa tcaggccaac cgcgagaata
    42421 atcaggcaca ccggcagcgc gcggatcagg tcgataagga tcgcttttgg tgtgttgagc
    42481 tggctatcgt tacgcaggga acccacagcg gaagccagtt tctgttgata cgctttcagc
    42541 cagcccagac gccagtggat cagcccggca atcaacagca gcggcaaacc ggcgaggaaa
    42601 gcgataaaaa cggcaggcca ggctttttcc cagttcaccg tgattttcat cgacttaaat
    42661 tcatctttca ggctttgcgg gaacgctttg atccagtccc agtccattgg acggttactg
    42721 ttcacccaaa agatttgctg agtcaggatg gatttcaggt ttttcgatac gctcattaac
    42781 tgctgctggt tgatttgcag gttaatggcc atcatcagct ggttacccaa ctgtttgttg
    42841 agttgatcca gcaattcgcg acgcatatca accacttgca ataacgcatc gtgaacttcg
    42901 ctgttgactt cgttggtgtg accttcttcc agtttgttga cgaacgcatc gctctggaag
    42961 agtgcatcac gctgctggtt aacttcaaac tgttcgagac gcaaatccgc gatgcggttg
    43021 gtcatgtttt ccagttcatc cgccgagggc agcgtttgtt gttgctggta aaggatacga
    43081 gacaacagca ggctgccctt caggacggca atctgctctt taatattgcg ttccgattgc
    43141 agcgcccgct ccagccagtt tttgacttta atgttttgct gcatcaactg attaccgttt
    43201 tcagtcgcgg taatcagacg ctgacttaac tgctggttaa tttccagttc ctgtttcacc
    43261 agcggattag cctgaatacg cgcggcttca tccggggaga cggcttcctg cgccgttttt
    43321 tcagttaaag tcaggcgctt gctgtttacc gcttcttgca acagctgcaa ctggtgctcc
    43381 agacgggcgc tgttcgccgt cacgtaatca cgttgctttt gcaaggtatc ctgcaagacg
    43441 gtgttccctt ccaggctttt acgctgctgg tcaatctccg cattcagcaa cgcctgctga
    43501 gcctgcatta acactttctg gctgggacgt aaggcagtct cgccgacatc agtcccatcc
    43561 agacgactgc gaatttgttg cagctgctgc gaagcgttat acatcgcatt ttgcacgcgt
    43621 tcgggctgcg tctgtaacga aaccagctgg ctgttataag acgccagatc gttttgtgcg
    43681 ttttgcaaat cgtccagcgc ctgggcaacg cgagtttcca gctggcgcaa cgaaagcgta
    43741 ctcagaattt tgcgcgtttc ttcgtcgtta tcgacatcgc taagtgctgt taacgccgcg
    43801 gtcgcctggc gcattttttc cggcgcttca gcgacttttt gccgtagctg aactgtctct
    43861 tcttttatgc gatcgatttt atcgagggtg gctaatgtat ctgtcagatc ctgctgcacc
    43921 agtttgtcct gagcagaaag atctttttgt ttatttagtg agtcaagttg cgcctgcagg
    43981 tccgcttttg tcggcagatc accattcgat gacgcccgcg caaacgccgt gttctggcat
    44041 aacaagacaa aaacaaaagc aataaatgct gaaaaaacaa aatgccgtga tcgtttgtaa
    44101 tactggaaca tagtcatgat gaatgaaggt ttctgaacct gaagaacgac ctgaaaaagt
    44161 caaaccgcaa gaatatcacg acgcagtgaa ccagaatagc aacgacgaaa atgtccagga
    44221 aaaatcctgg agtcagattc agggttattc gttagtggca ggattacgaa gcgtggggca
    44281 caggagatac atctccagta agatggcgac gtaatcgcgg gcttcttttt taagatcaaa
    44341 agattgcggg gcaaagagcc agttttccat caggccggaa atatagccgc gcataataat
    44401 tgctgcccga cgcgtcatta aatccgcagg caacattttc gcttcaatac aatgttttaa
    44461 cgtttgttct atacggtcat aactttccag acagagatta cgttgtgcct gttgcacaac
    44521 agccatttct ccgacaaatt cgcatttgtg gaatataatc tccatcaata atcgacgtcg
    44581 ttcttctgtc accgtggatt caagaacatg aattaatatc tctcttaata ctgagagtgg
    44641 atcgccaggg aattttgcct gatactcaag ctctagttca ccaatattgg attctgacag
    44701 ttcccagatc tcactgaaca aatccgactt gtctttaaaa tgccagtaga ttgcaccgcg
    44761 cgtaacgcca gctgcttttg caatctcgcc cagcgaggtg gatgataccc cctgctgtga
    44821 gaaaagacgt agagccacat cgaggatgtg ttggcgcgtt tcttgcgctt cttgtttggt
    44881 ttttcgtgcc atatgttcgt gaatttacag gcgttagatt tacatacatt tgtgaatgta
    44941 tgtaccatag cacgacgata atataaacgc agcaatgggt ttattaactt ttgaccattg
    45001 accaatttga aatcggacac tcgaggttta catatgaaca aaaacagagg gtttacgcct
    45061 ctggcggtcg ttctgatgct ctcaggcagc ttagccctaa caggatgtga cgacaaacag
    45121 gcccaacaag gtggccagca gatgcccgcc gttggcgtag taacagtcaa aactgaacct
    45181 ctgcagatca caaccgagct tccgggtcgc accagtgcct accggatcgc agaagttcgt
    45241 cctcaagtta gcgggattat cctgaagcgt aatttcaaag aaggtagcga catcgaagct
    45301 ggtgtctctc tctatcagat tgatcctgcg acctatcagg cgacatacga cagtgcgaaa
    45361 ggtgatctgg cgaaagccca ggctgcagcc aatatcgcgc aattgacggt gaatcgttat
    45421 cagaaactgc tcggtactca gtacatcagt aagcaagagt acgatcaggc tctggctgat
    45481 gcgcaacagg cgaatgctgc ggtaactgcg gcgaaagctg ccgttgaaac tgcgcggatc
    45541 aatctggctt acaccaaagt cacctctccg attagcggtc gcattggtaa gtcgaacgtg
    45601 acggaaggcg cattggtaca gaacggtcag gcgactgcgc tggcaaccgt gcagcaactt
    45661 gatccgatct acgttgatgt gacccagtcc agcaacgact tcctgcgcct gaaacaggaa
    45721 ctggcgaatg gcacgctgaa acaagagaac ggcaaagcca aagtgtcgct gatcaccagt
    45781 gacggcatta agttcccgca ggacggtacg ctggaattct ctgacgttac cgttgatcag
    45841 accactgggt ctatcaccct acgcgctatc ttcccgaacc cggatcacac tctgctgccg
    45901 ggtatgttcg tgcgcgcacg tctggaagaa gggcttaatc caaacgctat tttagtcccg
    45961 caacagggcg taacccgtac gccgcgtggt gatgccaccg tactggtagt tggcgcggat
    46021 gacaaagtgg aaacccgtcc gatcgttgca agccaggcta ttggcgataa gtggctggtg
    46081 acagaaggtc tgaaagcagg cgatcgcgta gtaataagtg ggctgcagaa agtgcgtcct
    46141 ggtgtccagg taaaagcaca agaagttacc gctgataata accagcaagc cgcaagcggt
    46201 gctcagcctg aacagtccaa gtcttaactt aaacaggagc cgttaagaca tgcctaattt
    46261 ctttatcgat cgcccgattt ttgcgtgggt gatcgccatt atcatcatgt tggcaggggg
    46321 gctggcgatc ctcaaactgc cggtggcgca atatcctacg attgcaccgc cggcagtaac
    46381 gatctccgcc tcctaccccg gcgctgatgc gaaaacagtg caggacacgg tgacacaggt
    46441 tatcgaacag aatatgaacg gtatcgataa cctgatgtac atgtcctcta acagtgactc
    46501 cacgggtacc gtgcagatca ccctgacctt tgagtctggt actgatgcgg atatcgcgca
    46561 ggttcaggtg cagaacaaac tgcagctggc gatgccgttg ctgccgcaag aagttcagca
    46621 gcaaggggtg agcgttgaga aatcatccag cagcttcctg atggttgtcg gcgttatcaa
    46681 caccgatggc accatgacgc aggaggatat ctctgactac gtagcggcga atatgaaaga
    46741 tgccatcagc cgtacgtcgg gcgtgggtga tgttcagttg ttcggttcac agtacgcgat
    46801 gcgtatctgg atgaacccga atgagctgaa caaattccag ctaacgccgg ttgatgtcat
    46861 taccgccatc aaagcgcaga acgcccaggt tgcggcgggt cagctcggtg gtacgccgcc
    46921 ggtgaaaggc caacagctta acgcctctat tattgctcag acgcgtctga cctctactga
    46981 agagttcggc aaaatcctgc tgaaagtgaa tcaggatggt tcccgcgtac tgctgcgtga
    47041 tgtggcgaag attgagctgg gtggtgagaa ctacgacatc atcgcagagt ttaacggcca
    47101 accggcttcc ggtctgggga tcaagctggc gaccggtgca aacgcgctgg ataccgctgc
    47161 ggcaatccgt gctgaactgg cgaagatgga accgttcttc ccgtcgggtc tgaaaattgt
    47221 ttacccgtac gacaccacgc cgttcgtgaa aatctctatt cacgaagtgg ttaaaacgct
    47281 ggtcgaagcg atcatcctcg tgttcctggt tatgtatctg ttcctgcaga acttccgcgc
    47341 gacgttgatt ccgaccattg ccgtaccggt ggtattgctc gggacctttg ccgtccttgc
    47401 cgcctttggc ttctcgataa acacgctaac aatgttcggg atggtgctcg ccatcggctt
    47461 gttggtggat gacgctatcg ttgtggtaga aaacgtggag cgtgttatgg cggaagaagg
    47521 tttgccgcca aaagaagcta cccgtaagtc gatggggcag attcagggcg ctctggtcgg
    47581 tatcgcgatg gtactgtcgg cggtattcgt accgatggcc ttctttggcg gttctactgg
    47641 tgctatctat cgtcagttct ctattaccat tgtttcagca atggcgctgt cggtactggt
    47701 agcgttgatc ctgactccgg ctctttgtgc caccatgctg aaaccgattg ccaaaggcga
    47761 tcacggggaa ggtaaaaaag gcttcttcgg ctggtttaac cgcatgttcg agaagagcac
    47821 gcaccactac accgacagcg taggcggtat tctgcgcagt acggggcgtt acctggtgct
    47881 gtatctgatc atcgtggtcg gcatggccta tctgttcgtg cgtctgccaa gctccttctt
    47941 gccagatgag gaccagggcg tatttatgac catggttcag ctgccagcag gtgcaacgca
    48001 ggaacgtacg cagaaagtgc tcaatgaggt aacgcattac tatctgacca aagaaaagaa
    48061 caacgttgag tcggtgttcg ccgttaacgg cttcggcttt gcgggacgtg gtcagaatac
    48121 cggtattgcg ttcgtttcct tgaaggactg ggccgatcgt ccgggcgaag aaaacaaagt
    48181 tgaagcgatt accatgcgtg caacacgcgc tttctcgcaa atcaaagatg cgatggtttt
    48241 cgcctttaac ctgcccgcaa tcgtggaact gggtactgca accggctttg actttgagct
    48301 gattgaccag gctggccttg gtcacgaaaa actgactcag gcgcgtaacc agctgcttgc
    48361 agaagcagcg aagcaccctg atatgctgac cagcgtacgt ccaaacggtc tggaagatac
    48421 cccgcagttt aagattgata tcgaccagga aaaagcgcag gcgctgggtg tttctatcaa
    48481 cgacattaac accactctgg gcgctgcatg gggcggtagc tatgtgaacg actttatcga
    48541 ccgcggtcgt gtgaagaaag tttacgtcat gtcagaagcg aaataccgta tgctgccgga
    48601 tgatatcggc gactggtatg ttcgtgctgc tgatggtcag atggtgccat tctcggcgtt
    48661 ctcctcttct cgttgggagt acggttcgcc gcgtctggaa cgttacaacg gcctgccatc
    48721 catggaaatc ttaggccagg cggcaccggg taaaagtacc ggtgaagcaa tggagctgat
    48781 ggaacaactg gcgagcaaac tgcctaccgg tgttggctat gactggacgg ggatgtccta
    48841 tcaggaacgt ctctccggca accaggcacc ttcactgtac gcgatttcgt tgattgtcgt
    48901 attcctgtgt ctggcggcgc tgtacgagag ctggtcgatt ccgttctccg ttatgctggt
    48961 cgttccgctg ggggttatcg gtgcgttgct ggctgccacc ttccgtggcc tgaccaatga
    49021 cgtttacttc caggtaggac tgctcacaac cattgggttg tcggcgaaga acgcgatact
    49081 tatcgtcgaa ttcgccaacg acttgatgga taaagaaggt aaaggtctga ttgaagcgac
    49141 gcttgatgcg gtgcggatgc gtttacgtcc gatcctgatg acctcgctgg cgtttatcct
    49201 cggcgttatg ccgctggtta tcagtactgg tgctggttcc ggcgcgcaga acgcagtagg
    49261 taccggtgta atgggcggga tggtgaccgc aacggtactg gcaatcttct tcgttccggt
    49321 attctttgtg gtggttcgcc gccgctttag ccgcaagaat gaagatatcg agcacagcca
    49381 tactgtcgat catcattgat acaacatgta atcactaagg ccgcgtaagc ggcctttttt
    49441 atgcataacc tacgaacatt aaggagtaat tgaaccacca actcaggatc tcatacgaaa
    49501 accagtatta accacgtata aaattcataa aaaatactga ttgttagtta atttatatta
    49561 agtagcgcta atagatttaa taatccataa tcatttagag gctattctta attatttgcg
    49621 gtaattcttt attcattcct cggttattac gtcatattca gagcaatcct ggtattagtg
    49681 tcaccaattt catctggcga taatcctgaa atgttatgaa cagttcgagc aaactgcttt
    49741 tacctgctgc gggttagtgc tagtatgaaa aagtgagtcc tgtcccgatt ccttcctaat
    49801 tgtaattttt cgtaataatg cgatgaaaac ctgcaaagag tggcttatag ttaagctaac
    49861 aaacgagagg gcaagtccaa gtcagtaagt tttttccatc ccgaaaggtg tccgttagtt
    49921 caaccactaa gaaggggacg cgttatggat gaatactcac ccaaaagaca tgatatcgca
    49981 cagcttaagt ttctctgtga aaccctgtat catgactgtc ttgcaaacct tgaagaaagc
    50041 aatcatggct gggttaacga cccaacctcg gcgatcaacc tccagttgaa tgaactgatt
    50101 gagcatattg cgaccttcgc acttaattac aaaattaagt ataatgaaga caataagctc
    50161 attgagcaga tcgacgaata tctggatgac acctttatgt tgttcagcag ttatggtatt
    50221 aatatgcagg ctcttcagaa atggcggaag ttaggtaatc gactattccg ttgttttgtc
    50281 aatgcgacga aagagaatcc tgcgagttta tcttgttaga attattacaa ccatgggtag
    50341 aagtatgtcc gaaaaacctt taacaaaaac cgattattta atgcgtttac gtcgttgcca
    50401 gacaattgac acgctggagc gtgttatcga gaaaaataaa tacgaattat cagataatga
    50461 actggcggta ttttactcag ccgcagatca ccgcctcgcc gaattgacca tgaataaact
    50521 gtacgacaag atcccttcct cagtatggaa atttattcgc taataaataa ttcgctttcg
    50581 gagctataac cggctgttta ttaagaattt tatacttttt cgccatgaag acatacccta
    50641 tgtgatcttt atcacacaga tgtaatgaga acgttctctt cactgacttt tcgtcttact
    50701 gtgttgccgc attttcagca accggaggtc agtaatgagc acagaaaaag aaaagatgat
    50761 tgctggtgag ttgtatcgct cggcagatga gacgttatct cgcgatcgcc tgcgcgctcg
    50821 tcagcttatt caccgataca atcattccct ggcggaagag cacacattac gccagcaaat
    50881 tctcgctgat ttattcggtc aggtgacaga ggcttatatt gagccaacgt ttcgctgtga
    50941 ttatggctat aacatttttc tcggcaataa ttttttcgcc aacttcgatt gcgtgatgct
    51001 tgatgtctgc cctattcgca tcggtgataa ctgtatgttg gcactaggcg ttcatatcta
    51061 cacggcaaca catcccatcg accctgtagc acgtaatagc ggtgctgaac tggggaaacc
    51121 cgtcaccatc ggtaataacg tctggattgg cggacgcgcg gtcattaacc ctggtgtgac
    51181 cattggtgat aacgtcgtgg tagcttcagg tgcagttgtc acaaaaggtg tcccggacaa
    51241 cgttgtcgtg ggcggtaatc cagccagaat aattaaaaaa ttgtaatcgg tttttcgcaa
    51301 ctgttatgca aaattgtggt agatctgtta cttcccctct actattccca cgttaaaata
    51361 gggtgttccc tggaaagttg cagataccac gaaggcaaac gatgaccgaa atacaacgcc
    51421 tgctgaccga aacgattgag tctctgaata cccgcgaaaa acgcgacaac aaaccccgct
    51481 ttagtatcag ttttatccgt aaacatccgg ggctgtttat cggtatgtac gttgcttttt
    51541 ttgccaccct ggcggtgatg ttgcagtccg aaacgctgtc aggctctgtc tggctactgg
    51601 ttgtattatt tatcctgctt aatggtttct tctttttcga tgtctaccca cgctaccgct
    51661 atgaagatat cgacgtgctg gatttccgcg tttgctataa cggcgaatgg tacaacacgc
    51721 gctttgtacc tgccgcgctg gttgaagcca tcttgaactc tccgcgtgtc gcggatgttc
    51781 ataaggaaca actgcaaaaa atgatcgtcc gtaaaggtga actgtctttt tacgatattt
    51841 ttaccctcgc tcgcgccgaa tcaacatctt aagttagggt tacataccag gcgtaaagct
    51901 ctgcgcctgg tcaaatgaca atgatcgttt ccacccatca cttcatgaaa taccagctct
    51961 acctccttat ctcctgccag cctttttcca caatcagata tactttccct acactgtgtt
    52021 aataaggata tgctggtgag aacacgacat ctggtcggcc ttatttcggg agtactgatt
    52081 ctttcagtat tgctgcctgt cggcttaagc atctggctgg cccatcagca ggtagaaaca
    52141 tcgtttattg aagagctgga tacctattcc tcccgcgtcg ctattcgagc caataaggtg
    52201 gcgacacaag ggaaagatgc gctgcaggag ctggaaagat ggcaaggcgc tgcctgtagc
    52261 gaagcccatc tcatggaaat gcgtcgggta tcttacagtt atcgctatat tcaggaagtg
    52321 gtttatatcg ataacaacgt tcccctgtgt tcgtctctgg agcatgaaag tccgcccgat
    52381 acctttcccg agccaggtaa aatttcgaaa gatggttatc gtgtctggtt aacatcgcat
    52441 aacgatttag gcattatccg ttacatggtc gccatgggaa cggcacatta tgtcgtcatg
    52501 atcgaccccg cttcctttat tgatgtcatt ccctatagct catgacaaat tgatgccgcc
    52561 attattggca atgcccataa cgttgtcata accagcagcg atgaaattgc tcagggaatt
    52621 attaccaggc tacaaaaaac acccggtgag catatcgaaa ataatgggat catttacgat
    52681 atcctgccct tcccggagat gaatatttcg atcatcacct gggcttcaac gaaaatgttg
    52741 cagaaaggct ggcatcggca agtctttatt tggttaccgc tcgggttggt gattggcctg
    52801 ctggcagcga tgtttgtgct gcgtattttg cgccgtatcc agtcaccgca tcatcggctg
    52861 caggatgcta tcgaaaatcg tgatatttgc gtgcactatc agccgattgt ctccttagcc
    52921 aatggcaaaa ttgtcggtgc tgaggcactg gcgcgctggc cgcagacaga cggtagttgg
    52981 ttgtcaccag atagttttat tccgctggca cagcaaacgg gcctttctga gccattgacg
    53041 ctactgatta taagaagcgt ctttgaagat atgggcgact gcttgcgtca gcatccacag
    53101 cagcatattt cgatcaatct tgaatccacc gtgctcacct cggaaaaaat cccgcaattg
    53161 ctgcgtgaaa tgatcaatca ctatcaggtt aatcccagac agatcgcgct tgaactcact
    53221 gaacgcgagt ttgccgatcc gaaaaccagc gccccgataa tttctcgcta ccgggaggcg
    53281 ggccatgaaa tttatcttga tgattttggt acggggtatt caagtttaag ttatttacag
    53341 gatctggatg tcgacattct gaagatcgat aaatctttcg ttgatgcgct ggaatataaa
    53401 aatgtcacgc cgcatatcat cgaaatggca aaaacactga aactgaaaat ggtagcggag
    53461 ggaatcgaaa ccagtaaaca agaagagtgg ctacgccagc atggcgtgca ctacggtcag
    53521 ggctggctct acagcaaggc attaccgaaa gaagatttct tacgctgggc cgagcaacat
    53581 ttgtgaattt ttgggctgac ggcacgccgc acagcccgtc acgctaccta ttcgtcgatg
    53641 attgactcga atccgccata gatcattcgc ttgccgtcaa acggcatgga ctcgccgaac
    53701 tctttcatcc gtgggtccga catcatcttt tgattagctg cgtcgcggac ctcttttgaa
    53761 gggtattcaa tccagctaaa gacaacctct tcattctctt ccgctttcac cgccatacga
    53821 aaatcggtca ctttgccatc cggtacatcg ctggcccagc attcgacaat acgaagcgcg
    53881 ccaaactctt taaacaatgg cgcggcctta gcggccattt cccgataggc atccttttta
    53941 tcggcaggaa cggcaaccac aaaaccatca acatacttca tcagacatac ctccgaagcg
    54001 tactgcgcag ccagacaatt cttgcgacgt cagttgtggt taaagtttag ccatgctggg
    54061 attttttgac gaaacggtgg gaggcagaaa tgggtggggg ccctgccagc tacatcccgg
    54121 cacacgcgtc atctgccttg gctgcttcct tccggacctg acctggtaaa cagagtagcg
    54181 ttgcgggaga accaacagag cccccattga gagcgttgaa aaccaacgcg caggcgcatt
    54241 atcactgctg ccccggctaa ttgcaaccca ttgcgtgcca gatgctggtt tcttgcgcaa
    54301 tgcgacttca ctcgggcgtt tttccagatt atgctgaaaa attacctcag gaagaaaaga
    54361 tggaaaaaga agattcattt ccccaacgcg tctggcaaat cgtcgccgct attcccgaag
    54421 gctatgtcac cacttacggt gatgtggcga aactggcggg atcgccccgc gccgcgcgcc
    54481 aggtgggcgg tgtgttaaag cgtctccctg aaggcagcac cttaccctgg caccgggtgg
    54541 ttaatcgcca tggcacaatt tcgctaaccg ggccggattt acagcgtcag cgacaggcat
    54601 tactggcaga aggtgtgatg gtatcgggaa gcgggcaaat cgacttgcag cgttatcgct
    54661 ggaactacta accctctccg ggcggagagg gttaacgtcg cttaatattg cgtaggagcg
    54721 ggaactgccg aagacggatt cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg
    54781 ccaccgctgg cctgaaccgg cacggcggtt tgctgtaccg gcaccaatgt caggtcggct
    54841 ttagttccgc cctggttgat caccggctga acggtatcgg tgataaatac cagtttgtca
    54901 ttcacgttaa tcgccgcact caacagaata cgcgcgttcg gctgaacatc tgccgggtta
    54961 aatggcagaa caaagctgaa tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc
    55021 agcacttttg acggtgcatc ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca
    55081 tcaggcggca gtgcgacttt ctgacggatc cagacggtac cggagacatt cggttgctgg
    55141 atagcaggtt gttgtgttgc tgaaatagac gtatttgcag ccggggctgg cgtctgaata
    55201 tccgcgcttt tatctgcgca agccgccaac gcaatcgcaa ccgctaaacc actggccatg
    55261 tgcacgagtt tcattcattt ctccttatta tcaatgcacc agcgggctaa ctttcctcgc
    55321 cggaagagtg gttaacaaaa tagtaacgtc aacaagtgtg gcacacatca cgcatttctg
    55381 cctgtaatta gcccgtaatt cagactattg cacccatcgg accagtggca aatttgcgtt
    55441 atactcaact cactttggct tgctgcggca gctttgttac tggagagtta tatgagtcag
    55501 gcgctaaaaa atttactgac attgttaaat ctggaaaaaa ttgaggaagg actctttcgc
    55561 ggccagagtg aagatttagg tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc
    55621 ttgtatgctg caaaagagac cgtcccggaa gagcggctgg tacattcgtt tcacagctac
    55681 tttcttcgcc caggcgatag taagaaaccg attatttatg atgtcgaaac gctgcgtgac
    55741 ggtaacagct tcagcgcccg ccgggttgct gctattcaaa acggcaaacc gattttttat
    55801 atgacggcct ctttccaggc accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc
    55861 gcgccagcgc ctgatggcct gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg
    55921 ctgccgccag tgcttaaaga taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg
    55981 gagtttcata acccactgaa aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc
    56041 gcaaatggta gcgtgccgga tgacctgcgc gttcatcagt atctgctcgg ttacgcttct
    56101 gatcttaact tcctgccggt agctctacag ccgcactgca tcggttttct cgaaccgggg
    56161 attcagattg ccaccattga ccattccatg tggttccatc gcccgtttaa tttgaatgaa
    56221 tggctgctgt atagcgtgga gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt
    56281 gagttttata cccaagacgg cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt
    56341 aatcacaatt aaaaaaatag ccggaggtga aaaccgtccg gctatttttt gcagtgcttg
    56401 ttacgcgtta taggcattct cgccgtggct gttgacgtcc agcccttctc gctcttgttc
    56461 ttccggcaca cgtaggccaa ccgtcagatc cgccagtttg tagccaataa atgccaccac
    56521 accggaccag acgatcgtaa tggcgatgct ttccaactgt accagcaact gatggcccat
    56581 cgtcacacct tcagcgaagc ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat
    56641 gatacagccg acaatgccac aaacgccgtg cacaccgaag acatcgcagg gatcatccac
    56701 ccgcagcaag cgtttgagca tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc
    56761 gataatcaac gcgccgccaa ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc
    56821 ggcaatcgcg ccagaacacg ctcccagcag agaaggctta ccacgcagcg cccattcacc
    56881 gaagatccag ccaagaattg ccgcctccgt tgcgaccaca gtattcacaa atgccagtgc
    56941 cgcgatttca ttcgccgtgc ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag
    57001 aatggcagtc ccggtgaaga ccatcggcag gttgtgcggc ttaaacgcct ctttaccgaa
    57061 gcccacgcgt ttgcctatca gatacgcgcc caccagaccg gcgattgctg cgttaatgtg
    57121 caccaccgtg ccacccgcga aatccagcgc accgtgagaa gccagcaaac caccgcccca
    57181 caccatatgc gcaatcggaa tgtaagagag cgtcagccat accaccacga aaatcaacac
    57241 agctgagaag cggattcgtt ccgccagcgc cccaactatc aagcctacgg taatgcaggc
    57301 aaacgatccc tgaaacgcta cgtggatata ctgataaatg ctgcccatca ccgccgtcag
    57361 ttcgatgttt tttagcatca accagttaat gttgccgaag aagttgttgc cctcaccaaa
    57421 cgccagcgaa taaccgtaaa ccacccagag aatacagacc agtgcaaacg tcaccgtcac
    57481 ctgcgtcagc atcgacagca cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat
    57541 ccccggaata gtcataaaca gcaccagcgc agtacaaatc atcataaacg cattgtcggc
    57601 tttatcggcc accgcaggtg cagccattac cagtcccggc agcatcgcca gtgaagcaag
    57661 cccagttttt atcgtcgcta tcttcatttt tcgttcctgt tgctgtgtgc cagagattac
    57721 agcgccgctt cgtcggcttc gccggtacga atacgaatga cgcgttgcaa ttcagcgacg
    57781 aagattttgc cgtcgccaat ttttccggtg taagccgcct tactgacgat atcgatcact
    57841 tcatcgagtt ggtcatcagc aatcgccaca tcaattttta cttttggcag gaagttgacg
    57901 ctgtattccg ccccccggta cagctcggca tgccctttct gacgcccgaa acctttcact
    57961 tcggtgacgg tcaggccctg aataccaatg gaagataacg cttcacgaac gtcttccagc
    58021 ttgaatggtt tgattatcac ggtcaccagc ttcatagatc ccctccggtc agaattcggt
    58081 aatggttctg ctacacgaag aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa
    58141 agagagcagg aagttaagaa gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt
    58201 tacatttttg caccaacgat gaaatattgc gctattcagg cgctcaatga ttcctcttca
    58261 cgcacgctgg ctgccagctc ttcgcccgca agttgcagtt gatacatctg ccagtagcgt
    58321 ccctgggccg ccagcagttg ctggtgagtg ccctgctcca cggcttgccc acgatgaagc
    58381 accagaatgg tgtcggcatc aacaatagtt gataagcggt gagcaatcac caccagcgtg
    58441 gtatgttcac gcaccgccgc cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg
    58501 ctggcggttg cctcatcaag gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt
    58561 gccagcagtt gcttttgccc gactgagaga ttatttccct gctcgcccag cggcgtataa
    58621 ataccatcac tcatgctgcg cgccagctcc gccagttgca cggtttccag cgcctgccag
    58681 acgcgttctt cggagatatc ccgccccagc gtcacgttgg cgaggaaggt atccgccagc
    58741 accaccggat cttgctgcac cattgccacg ccctggcgca gcgcgctatg acttagcgaa
    58801 cttaatggac gaccatcaag gtgaatctca ccttccgtta gcgggtaata gcccatcaat
    58861 aaactggcga gggtactttt accactgccg gtatgcccga ccagcgccac aaagttacgc
    58921 gaaggcacag agagattaat gttctttagc accagattgt catcgcgata agcaaatgac
    58981 acgttatcga cttcgatggt gccactctgt aacgggtaat catcattgcc atattgctgg
    59041 cgcggtccgt ccatcagttc aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc
    59101 gcctgttgcg tggtcagttc gattaagggt tcgttaagtc gcccaagata gctgataaac
    59161 gcatacaaca cgcccacttc aatggtgccg ctggcggaga agccaaacag catcaataag
    59221 ccacaaagaa tgagcgatga aaacagactc agcagcggac gcagcagaaa accgtcgagg
    59281 cgcagggttt gcatcctcgc catatagtgt gaacgactgg cctcccccat acgttcgcca
    59341 aatcgcgcct gctgacgaaa ctgctggata acgctcatgc cattgatgat ttcgttaaag
    59401 ccgtcgttga tatccgccag ataggcgcgc acacggcgga caatcggcgt gctgtaacgc
    59461 tggtatatca ccattaccac cagcaccacc gggaaaatca ttatcgccac cagcgccatt
    59521 cgccagtcga ggctgaacat cgccaccagc atcgcgccca ccagtgcggc actgcgcagg
    59581 acagttgcca ctacggtaac gtagagatcg cggatcactt cagtgtcatt agtgacgcgg
    59641 gaaatcacct gcccgacggg ttgggtatca aactcgctta atggctggcg taacgcagca
    59701 tccatcacgt cggtacgcaa ctgttgcact acgcctactg ccgcccgatt aaacagcagc
    59761 gactgcgcgt aatgtagccc ggcggcaaac agttgtagcc caacatacgc cgcagccagc
    59821 cctgcaacca ctttcaacgg caggttattt ttcgctacca tattgtcgat aaaatagctg
    59881 ataagcagcg gcccactgac ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc
    59941 cccagcggtt tacgccacgg cgaaccatac gctaacaggc gcttgagagt cggccacagt
    60001 tggctaaaac tacgcatcga cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc
    60061 ctccagttgt tgatagcgat acatatcgcg ataccagccg ctttgttgcg ccagctcatc
    60121 atgattgcca cgctgggcga tatgtccgtg ctgcatcaca ataatttcac tggcttccgt
    60181 cagtgccgaa aggcgatggg cactgatgat taccgttctt ccctgtcccc actgacgcag
    60241 gttatgcagg atctggtgct ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat
    60301 gaggatttcc gcgttgacta ataacgctcg agcaatggag atacgctgtt tttgcccacc
    60361 ggaaagcatc acgccgcgct cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat
    60421 atcgtcatgt acgctggcta accgcgcgac atgctcaatc tcttgctggg tggcattcgg
    60481 gcaacccagc gcgatgttat tcgccacggt gtcagaaaaa aggaatggcg tctggctgac
    60541 caccgccagg cggctacgcc agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa
    60601 gcgaatatcc ccctcgctga cgtcgaaatg acgctgaatg agcgacaaca gggtactttt
    60661 gccggaacca gtcggcccgc aaatacccag catctgaccg ggtttcagga cgaaattgac
    60721 gttttccagc gcaggatggt cagtctgcgg atacgtgaac tggtgaatat ttacatccag
    60781 ttcgccacgc ccttccggca cgggttcgct accatcgttc accaccggcg cttccgccag
    60841 catcgcgcga atacggctgt atgcagcact accacgttcc acaatgttaa acatccatgc
    60901 cagcgccagc attggccaaa tcatcagacc taaatacatc ataaaactgg tgagctggcc
    60961 cagcgttaaa ctgccctgca ccaccatcca actaccgccg ccaatcgcca gcaagttcgc
    61021 cataccaatc gcgatataga tggtcgggtc gaaacgagca tcaatacgcg ccacccgcat
    61081 gttttttttg ccggtatctt tggcatccac ggcaaataac gccgactggc gatcttccag
    61141 accaaaggct ttgatcatgc ggatactggt gaggctttcc tgggtgcggt cattaagact
    61201 ggaaaacgcc gcctgtgcca gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat
    61261 catgatcgcc atcactggca tcggtaacag ggcaaataag gtcaactgcc agctaatttg
    61321 cgtagacatc ataatcaaca cagcgcagcc cattaccagt gaatccacca gcgtcagcac
    61381 cccttctccg gcggcaaaca cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc
    61441 acccgtgcga tgacgcaggt aaaactccgg gtgctgccgg cttagctgac ggtaataatc
    61501 ttcacgcagt tcaacagcca gttggtaaga cgcgccaaac agcaatacgc gccagacata
    61561 acgcaggaga taaaccacaa cggcaatcag caccatggtg gcgatccaca tcaggatcag
    61621 cccggtagta aagtgttgtt ctgtcacgcc atcgacaaca ataccaacca cttttggcgg
    61681 aaccagttgc agcatcgcga taatgacaag caaggcgaca gccccaagat agcgacgcca
    61741 ttcccgacgg aaataccagc ttaattgagc aaataatcgc acgcgttgtg tcctgacctg
    61801 attctggata tttattcgat gggtaaagaa gtggtgtatt taatctgttc catcgcgaag
    61861 ctggaagtga cgtccgacag ccccggcacg ctgtttacca gacgcttata aaactcgtcg
    61921 tagcgtttca tgtcggcaac ctggacgcgc atcagataat cgtattcacc agccatgcgc
    61981 cagaacccca gcacttctgg catttcggta accaccgtga caaagcggca ataccattcg
    62041 ctgctgtgat gttgcgtttt tatcagcaca aaggcggtca ggccgaggcc tattttttcc
    62101 ggatccagca gggcgacttt gccgataagg ataccgtcgt cctccagccg tttcaggcgc
    62161 ttccagcagg gggtggttgt cagattaacg gcttcagcca gtgcctgcaa agagagggtg
    62221 caatcctgct gcagtaaggc cagcagctta cggtcaattt tatctaacat agcccttcca
    62281 cggagaattt ttttctcttt taattctatt ttaaaggtga aatcgcgaca atttattctg
    62341 tgcggtttaa acaccacggc acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt
    62401 gcccggtgct ggaagggatc tcgttattcg ggggaataag gtagatgtgg atagtccagc
    62461 cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac cggtaaatgc
    62521 gggagctccg cgcgcagttg cggcatcgca ttgcccataa taaacccgct accgacgctg
    62581 cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg caacgataaa
    62641 cctaaatgtt gggtcagcac cgtcaatgca gcgcctttat tgcagcccac cggcagcact
    62701 tcgaggcaat acgtggcgga aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc
    62761 tggatctgca agcgtgtaag atcgtcgtga tcgccacaga agcagatctt ggtgacgctg
    62821 ccgagtggca tttttttgac atcgattatc tgataacgaa aaccgctata gacaaatgcc
    62881 tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa gatatgcatg
    62941 ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc aggtaaatca
    63001 tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa
    63061 tatgcatcca gcgatagcgc cccgagaata tgctgcatct ccagcgcatg acgccccgtg
    63121 gcaaaagtga gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag ggttttctca
    63181 cctaaatgat ggtcaggcat caataaagtg ccatccatat caaatgctgc cagacgagcc
    63241 atttctttct ccactctgtg acacggttaa tggtgattga gtatcacctg atatatacgg
    63301 aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgattg ctcaaccgtc
    63361 ttaaccagta tcaacgtctg tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca
    63421 gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg
    63481 cacaggaggc gggatggctg gagtggcagg cgcagtcagg acgcggaaag cgcggacaat
    63541 tacgctttct ggtcacgccg gaatcgctac gcaatgcgat gatggaacag gcactggaaa
    63601 ccggaaagca gcaagatgtg ctggagctgg cgcaactggc cccaagtgag ctgcgcactc
    63661 tgttacagcc gtttatgggc ggacaatggc aaaacgatac acccacgttg cgtattccct
    63721 actatcgccc gctcgaaccg ctacaaccag gctttttgcc cggccgtgcc gagcagcatc
    63781 tcgccgggca gatattttcc ggcctgaccc gcttcgataa taatactcag cgcccgattg
    63841 gcgatttagc gcatcactgg gaaacctcta ctgacgggtt acgctgggac ttttatcttc
    63901 gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta caccagcgat
    63961 tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg aagcgtattg
    64021 aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccccgattac tggcttgcgc
    64081 accggctggc gagctattgc agccatctgg cgcatccgca attcccactg atcggcactg
    64141 gtccttttcg cttaacacaa ttcacagcgg agctggtgcg cctggagagc catgattatt
    64201 accatttgcg tcatccgctg cttaaagcgg ttgagtactg gataactccg ccgcttttcg
    64261 aaaaagattt gggaaccagt tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg
    64321 agctgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc tatttaacgt
    64381 tgcgcaaaag tccccgactc tccctctggc aggcgcgaaa agtgatctcc attattcatc
    64441 aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat
    64501 tactgccagg ctggactatt ccgcactggc aagtaccgga tgaagtcaaa ctaccgaaaa
    64561 ccttgacgct ggtttatcac ctaccgatag aacttcatac catggcagaa cgcctacagg
    64621 cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca aaaaactggg
    64681 acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta attggcgaag
    64741 caccggaata tactctggag caatggctac gttgcgatcc actatggcca catgttttcg
    64801 acgctccagc atatgcacat ctgcaatcga cgctggacgc ggtgcaagta atgcctgatg
    64861 aggaaaaccg atttaatgcc ctgaaagcgg tttttagcca gttaatggca gacgcgacgc
    64921 tgacgccgct gttcaactat cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc
    64981 gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat
    65041 gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt tcaagcagga
    65101 ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac cacctgtctg
    65161 gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta cggtcagcga
    65221 catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggagc acgcgcgcat
    65281 aaggttcttg atgtaacgct gctcaacgag ctggcggtca gcagcctgac acgtgacagc
    65341 attccggtgc ctgattatga acctgaagcc gatggcatcc cgaatacgtt tgtcccaggg
    65401 cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa agcagaagcc
    65461 gtaattaccg gcgtctgtga aacggatttc tccggctacc cggattgccg cgatgagttt
    65521 gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg ttttgaaacg
    65581 ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta cggcaaactg
    65641 gatttagtcc gtaacgaaac gttgacctgc tataacggca ttaaaggcga cggttgcggt
    65701 cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc cgataaaccg
    65761 acggtgatgg cagcgatgaa gcagaaaacc gggttgaggt aattattccg ggtgtcgccg
    65821 gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct ccagcttttc
    65881 gcgcaattcc ccttccagag ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt
    65941 aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct ggcttaagat
    66001 gccgctttta ccgtttaatt gctgcaactg actggtaata gttaacaggt cgcttaatac
    66061 cgctgggcga cgatagttaa tattgatatt gaccacgacg aaggcgatgt tatgggccgt
    66121 catccactga aaactgtcgc tattttccaa cccatcccag cgggcttctt cgagaaattc
    66181 aaggtagcgg gcgttgttga cgtgctggta aacgtcgaga tgatatccac gaactttgat
    66241 ttgtgtttgc atagcgcaat aaccttacgt tgttgttata agtacagagg tcataactgg
    66301 tatgacctct tcagtctggc aaaaaattgc cactatgcaa attaattaca gggttaatac
    66361 cgccagatta cgttccacca gcgaattgcc catccccggc acctgcttta gatcctccac
    66421 cgttttaaac ggaccgtact cttcgcgata actgacaatc gcctgcgctt tcttcaggcc
    66481 aacgccattc atcgcgcggg ctagctcttc cgcgctggca ttattaatgc tgacccgggt
    66541 gccttcttcg tcactggctt tcgccggtac tgctgcttta ctttgtgctg caggagcttc
    66601 cgctttggtt tctaccgccg tcggtttcgc cacagaagct gccgccagcg cgctatgaga
    66661 cattccggca caggccaggg acagggtaat gagcagtgct ttaattccgt gtttcatact
    66721 gttttctcct tgtttgttaa cagtggaatc accttaacgg gagtgaaaac agtaaacaaa
    66781 tggcaatgtt cagaaatgga aaaggccgcg aaagcgacct tttcttgtta cagctgcaac
    66841 gcattttttt gcgaggcggc ttcaggatta ttgctgttcc agcgcatcgc caattttgat
    66901 tttcgcctct ttacgcaggt tactcatcag agcttcaaag acgatttgtg cgttgttctg
    66961 ggtgataccc tgcaccatcg cttttttctg atcttccggc attgaacctt gtttcacttc
    67021 atccagcgcc agcagaacca cattaccttg catatcggtc gccataccgt agctcggttt
    67081 gtctttcgct ggcagtggca gtgcaaacgc cgcctggcta atcgggtcac gaccggaacg
    67141 gcttaaggtt ttcggctcgc caaatttcag accggcagcc tgcatagctt ccgcaccttt
    67201 gccggctttc aaatcaacca gcagtttctc agcatccact ttcgcctgtt gttcagcttt
    67261 gttgtgctga accagtgcct taacttgttc ctgaacatct gccaacggtt tcaccgcttc
    67321 cggtttgtgc tcgctgatgc gcagcacgaa tgcgcggtcg ccgtctacgg tgatgatgtc
    67381 agagttgatg cccggcgcgc cgttttcacc taccagaccg ccgttaaaga tagcgtcggc
    67441 aaccggcttg aagttcaact cttccggcag gttatctttg ctgaaccaac ccgtctgagt
    67501 ggctttaacg ccggcagctt gctctgcacc ggccagagac tcggtgtcgt tgcttgccgc
    67561 atcgctcact ttctgctgca gcgcatagta cgcatcgagg gctttttcgt gtttcacttt
    67621 cgccgcaatg tcgtcacgta cttcgtctaa cgatttcact ttcgctggct gaatgtcgtc
    67681 cagacgtaca atcaggaaac cgaccgaaga tttgatgaca ccagacagtt ggcctttttc
    67741 tttcagacca gcatttttca gttcgtcggg gatagtggca tcttctaacc aacccatatc
    67801 gccgccgtta cgggcagaga taatatcggc agatttttct ttggctaatg cagcaaaatc
    67861 accgccttta ttcagctcat caagtaccgc tttcgcttca tcttcagttt tggtctggat
    67921 gatgctgtag cgggtacgct gcggctgggt gaattgatcc tgatgctgat cgtagtagct
    67981 ctggatatcc gcatcgctaa ccggttgctg catcgttgcg gcaaccagct tgatgtaact
    68041 cacgcggaat tgttccggcg tcatgaaatt gtttttgttt tgttcgtagt agctggcaat
    68101 ctcctgttcg gtcacaggct gcttcgccgc cagcgcgtta acatcgatag tcgcctcacg
    68161 caccacgcgt tgttgcgcga ccagtgccgc cagctcgtcg gtttcacctt tcagcataaa
    68221 atcggtaccg gcaacgccgt taatcagctg ttgggtagtg agctggttac gcagcgcctg
    68281 ggcgtactga tcggcggtca tccccatctg gttgaggata ccgttatagc ggctgttatc
    68341 aaatttgccg tcaacctgga aggctggggt cgcgaaaatc gcctgtttaa cctgctcatc
    68401 actgataccc agtttcagct cacgagcgta ctgatccaac agcgcctcgt cgatcagacg
    68461 attcagcacc tgttgacgca gggttttcat atagccttcg ttcgctgcca gctcggagta
    68521 ttgatcgccc agctgttgct gcatgcgatt acgctcgctg ttaaaggcgt tctcgaactg
    68581 cccacggctg atttcctggt cattcacttt tgcggcgtag ttattgcctc cgccaatcag
    68641 gtaaccactc acgccggtca atatgaacga cacgataatg ataccgaaaa taatcttgag
    68701 cacgagactg tttgcagccg tgcgtaagct gtccatcatg gtgtaacaac actccgctgt
    68761 agatgactgt gtacctcacg caacgtatcg atgcgcgcaa aggcctattg tgacaagaaa
    68821 cgggggcaat tgtcagccca caagacacag aaagtcaccg aatacaaata aaaaaggcac
    68881 atcagtagat gcgcccttga acttcgtcac atccccactg gggacaacgc ttagtttacc
    68941 gcgtctttca gtgctttacc tgcacggaag ctcggtactt tagcggcagc gatggtgatc
    69001 tctttaccgg tctgcgggtt gcggccagta cgggcagcac gctctttaac ggcaaaagta
    69061 ccaaaaccta ccagtgctac atcatcccct tctttcagag attcagttac ggaagcaata
    69121 atagcatcta acgcacggcc agccgcagct ttagagatat cagcccctgc agcaatcttg
    69181 tcgatcaatt gagatttatt cactcttctc ttcctcttta taatttatat cgcacttgaa
    69241 tccttcaagg tacgaacgcg cagcagttat atcaggccag ccatcccctt acaacacccc
    69301 ttaataagga caagcccgat ccgctatcta acttagcgag acaaaaaaag gctggcaagc
    69361 ccgaattagc ctgccagccc tgtttttatt agtgcatttt gcgcgaggtc actattttgc
    69421 agtcacaacc tgcatgccag acggttcatt ttgcagcgcc agagtcagaa cttcctcaat
    69481 gcgcttcaca ggatgaatgt ccagatcggc aattacgttg tcaggaatct cttccagatc
    69541 gcgtttattt tcgaacggaa ttagcactgt tttaatcccg ccgcgatgcg ctgccaggag
    69601 tttttctttc aaaccaccga tcggcagtac ctgaccacgc agagtgatct caccggtcat
    69661 tgccacatcg gcacgaaccg ggttaccggt caggcaagaa accagcgcgg tgcacatagc
    69721 aataccggca ctcggaccat ctttcggcgt cgcaccttcc ggtacgtgga cgtggatgtc
    69781 acgtttttcg taaaaatcag ggttgatccc cagtttttcc gcacgcgcac gaaccaccgt
    69841 taacgccgcc tgaatagact cctgcatcac ttcgccgagc gaaccggtat aggtcagttt
    69901 gcctttaccc ggaacacatg cggtttcaat ggtcagcaag tcaccgccca cttccgtcca
    69961 cgccagaccg gttacctgac cgacacggtt ttcgttatca gcgcgaccat agtcgaaacg
    70021 ctgaacaccg aggtagtcat gcaggttatc gccgttaatt tcgatatgtt ttaatgactt
    70081 atcgagcagt aactgcttaa ccgctttgcg gcacagtttg gagatttcac gctccagacc
    70141 acgcacgccc gcctcacggg tgtagtaacg aataatgccg ataatggcgc tatcgtcgac
    70201 ggtcagctca ccttttttca gtgcattacg ttcaatctgc ttcggcagca ggtgacgttt
    70261 ggcgatgttc agtttttcat cttcggtata accggagagg cgaatcactt ccatacgatc
    70321 cagcagcggt gccggaatgt tcatggagtt cgacgtcgcg acaaacatca cgtcgctgag
    70381 atcgtaatcc acttccaggt agtggtcgct gaacgctacg ttctgctctg gatccagcac
    70441 ttcaagcagt gcagaggccg gatcgcctcg catgtcagaa gacattttgt cgatctcatc
    70501 gagcaggaac agcgggtttt tcacgcccac tttcgccatt ttctggatca gtttacccgg
    70561 catagaaccg atgtaagtac ggcggtgacc acggatttcc gcttcatcac gcacgccgcc
    70621 cagcgccata cggacatatt tacgcccggt ggctttggca atggactgac caagagaggt
    70681 tttacctacc cccggcggcc ctaccaggca gaggatcggt cccttgattt tgttgacacg
    70741 gctttgaacc gcaaggtact caaggatgcg atctttcacg cgctccagac cataatggtc
    70801 ggtatcaagg atttcctgcg cctgacgcag gtcttttttg accttgctgc gcgcattcca
    70861 cggtacctgt accatccagt cgatataacc acgcactacg gtcgcttctg ccgacatcgg
    70921 agacatcatt ttcagcttct gcaactctgc ttccgctttc tcttttgcct ctttcggcat
    70981 cttcgccgcg tcgattttgc gcttcagggc ttcgttttcg tccggcgcgt cgtccatttc
    71041 accgagttct ttctgaatag ctttcatttg ctcgttcaga tagtactcac gctgggattt
    71101 ctccatctgc tttttaacgc ggttgcgaat gcgtttctca acctgcagca gatcgatttc
    71161 cgattccatc attgccatca gatattccag acgttcgtta acgtcggaca tctccagaac
    71221 ggactgtttg tcagccagtt tcagcggcat atgtgcagca atggtatccg ccagacgcgc
    71281 cggatcgtcg atgctattca gcgacgtcag cacttctggt gggatttttt tgttcagctt
    71341 gatgtagcct tcgaactggc tgattgcagt acgcaccagc acttcctgtt cccgctcatc
    71401 aatggtcggc gactccagat actccgcctt cgcagaaaag tgttcgccat tgtcagagag
    71461 cgcagaaata cgcgcgcgct gtaacccctc gaccagcact ttgacggtgc cgtcaggcag
    71521 tttcagcatc tgcaatatag aggccacggt cccgacggtg aaaagatcgt ttacacccgg
    71581 ctcatccgtt gaagcttctt tctgcgcgac cagcataatt tttttatcat ggtccatcgc
    71641 cgcttccaga caacggatag atttttcccg cccgacaaat aaggggatga ccatgtgcgg
    71701 ataaaccacc acatcgcgca gcggcaatac ggggatttca atgcgttcag aacgctcagg
    71761 attcatagag ctctctctta gtttaatttc cgccaggtaa tcagatgaca cgactgtgct
    71821 tcacgccatc tattaacatg tacgtcagta tatggggatg tttcccccac attcaacgcc
    71881 gagaatagag gaaaaattaa aggggagata aaatcccccc tttttggtta actaattgta
    71941 tgggaatggt taattattca ccagatgcct gttgcgcttc cggcttacca taaatcagca
    72001 acggtttgct ttgaccatca attaccgact cgtcgataac cactttttcg acgtcttcca
    72061 tggacggcag atcgtacatg gtatcgagca gtgcggcttc tacgatggaa cgcaggccac
    72121 gggcaccggt tttacgcgcc atcgctttct tagcgatagc atccagcgcc tcgtcacgga
    72181 attccagatc cacgccttcc agattaaaca gcgcctgata ctgcttggtc agggcatttt
    72241 tcggctcttt gaggatctga atcagagctt cttcgctcag ttcattcaac gttgctacaa
    72301 ccggcagacg accaatgaac tcagggataa gaccaaactt gatcagatct tccggttcaa
    72361 cctgcgccag cagctcgcct tcgcttgctt tgtcggactt cgcttttacc gtcgcgccaa
    72421 aaccaatgcc ggagccggtt tctacacggt gggaaatcac tttatccaga ccggcaaacg
    72481 caccgccaca aataaacagg atcttagagg tatcaacctg caagaattcc tgctgcggat
    72541 gtttacgccc accttgcggt ggaacagcag ctaccgtacc ttcgatcagt ttcaacagtg
    72601 cctgctgtac tccttcaccg gaaacgtctc gggtaatgga cgggttgtct gacttacgag
    72661 aaatcttgtc gatttcatcg atgtagacaa tcccgcgctg cgctttctgt acgtcgtaat
    72721 cgcacttttg caacagcttc tgaatgatgt tttcaacgtc ttcgcccaca taaccggctt
    72781 cggtcagcgt ggttgcgtcg gccatggtga acgggacgtc cagcaggcgc gccagcgttt
    72841 cagccagcag cgttttaccg gaaccggtcg gaccgatcag cagaatgtta cttttgccca
    72901 actcgacacc attgctggta tcgccgttgc gcagacgttt gtaatggttg tataccgcga
    72961 ccgccagcac ttttttcgcc tgttcctggc cgataacgta atcgtccagg tggttgcgaa
    73021 tttcatgcgg cgtcggtagc gcactgcgtt cacgatgcgg tgcaacttct ttaatctctt
    73081 cgcgaatgat gtcgttacat aaatcaacac attcgtcgca gatatacacg gatggaccgg
    73141 caatcagctt gcgcacttca tgctggcttt tgccgcaaaa agagcaatac agcaatttgc
    73201 ctgagccatc tttgcgttta tctgtcatgg gtcaaaacct cttctttgtt ctttgtgccg
    73261 cacacgacga cgcaaatgcc attctcaggc gcaagccgct tacagcgttg tgccgcccta
    73321 gataagtata gcggcacagt tgcgcctctg gcatcaatta cgatgggtca gaatcgaatc
    73381 gaccagaccg tattccaccg cttcaggggc ggaaaggaag cgatcgcgct cggtatcacg
    73441 ttcaatctgt tctaatgatt gacccgtatg aagcgccata agttcattca tgcgcccttt
    73501 aactttcaga atttcacggg catgaatttc gatatcggtc gcctggccct ggtagccgcc
    73561 caacggttgg tgaatcatca cgcgcgaatt cggcaggcaa aaacgtttac cttttgcccc
    73621 tgcggtcaac aagaaagcgc ccatcgaggc cgcctggccc atacagatgg tgctgacatc
    73681 aggcttgata aactgcatgg tgtcatagat agacatcccg gcagtgatca ccccgcctgg
    73741 ggagttaatg tacagataga tatctttttc tgggttttcc gcttccagga acagcatctg
    73801 cgccacaatc aggttagcca tgtggtcttc aacctggcca gtcagaaaaa tgacgcgttc
    73861 cttaagtaga cgagaataga tatcaaaaga gcgctcaccg cgtgaggtct gttcaatgac
    73921 catcggcacc agcgccatat ggggtgcaaa gttatctcgt tcgccgctgt atgacatttc
    73981 cgtctcctgg ataaaattga aaaaaacctg ctgtaccgat tgtaacctta tggacggatt
    74041 atcagctagt ccctgtgtta tgggtacggc atcaccatat ttcaagcata acaatctttt
    74101 gttgttacgc taacaccgaa agaggctttt cgcacggctc catgcaaaat tcatgacaaa
    74161 aaaaagccca ccacctggcg gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt
    74221 aaattacgcc tgctggttca tcagctcgtt gaaagtggtt tctttttcag tcactttcgc
    74281 tttcgccagt acagcttcaa cagcctgttc ttccagagca acattgcgca tgttgtccat
    74341 cagttctttg tttttgctgt agaactcgat aacttctttc ggatcttcgt acgcagaagc
    74401 catctcttcg atcaggcctt tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat
    74461 aacttcgccc agcagcaggc caacaactac gcggcgttta gcctgttctt cgaacagttc
    74521 gcgcggcagt tccagagctt gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg
    74581 cagaacgtcg atttcgctgt cgatcagcgc agccggtacg tcgatgtcgt tagcttttac
    74641 cagaccttcg atcgcctgag acttaacgcg gttacggatg gcgctcttca gctcgcgctc
    74701 catattttta cgcacttcag cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg
    74761 tttgatgaat tctgcagtca gttccggcag ttcacgctct tcaactttct tcaggttgat
    74821 agcgaatttc gctgctttac ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac
    74881 gtcgatggtg aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccgggat
    74941 catacgaccc tggcccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc
    75001 gccgtctaca gaaccggtga agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc
    75061 tttttctttc caggtcgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc
    75121 gtcggtcact tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac
    75181 ttccggataa acttcaaact ctacagagta agtgaagtct tcacccagct tgtattcgcc
    75241 cggaacataa gtcggtgcgc cagccggatt gattttttct ttaatgatgg cgtcaatgaa
    75301 gttacggctc atcaggtcac ccagaacgtc ctggcgtaca gacgcgccat aacgctgagc
    75361 aacgatattc attggcactt tgcctttgcg gaagccgtca atacgtactt ttttcgcaac
    75421 gttgaccagc tcgcttttaa cagcggtctc gatgctgtca gcagcgatag taatcgttac
    75481 acggcggcca aggccttgag tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat
    75541 cacagtgctc ggtcaactct taccccgcaa gcacaggttg ttggcttcgc ggaaccggga
    75601 tgttcttata atcaatcaca ttccctgtta ccagaatcat cccgaaagca ttcaaatagg
    75661 acgcggcatt atagcggcat cacttttatg agtcgagaac ggttattgcg cgctgtcgcg
    75721 tattttttca caattcccgg gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc
    75781 agacaaaaca aaacggcccg caggccgttt ttcataaaat ctttagcaac atactggaaa
    75841 agcgccgaca gttgcaaatg cgtttttacg cgatgcttcc tgctccacga cagggaggag
    75901 aggcaaagac ggtgtcctgc aacccttccc actccttaat agtgtaagta tgcagagcca
    75961 gcgcatgaac ggtagtagag agttcctccg ctaaagtact gtaaatcatt cgatgacgat
    76021 tcagaaaacg ttcacccgta aaacgatcgc tgaccagcac aactttaaaa tggctttcag
    76081 agccggctgg gacattgtga cgatagcttt catccactac ttcgaggaat acgggttgga
    76141 acgccgccct taatttttct tctatccgct cacgtatcat catgaaatat ctcctccgac
    76201 aacgctgaga tgtcatccat ccctttaaat actagccgct tttaccgttt ccattgcagc
    76261 ttaacaacaa atatttaggt ttcgtcctgg tttttccgtc aagcgtatga agtttactga
    76321 tttaaagatt gaaacacagg cctcgcatca gcgtttcttt taccaggggt gattatttcg
    76381 tcaacatgat gaatttacat gccttaccca cttcccctcg ccgttggcaa tgttatgatg
    76441 gcgggaattt ttaccaaaca cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa
    76501 tcctcttccc gttagttgct ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta
    76561 ttgaagtttc cccgacgatt acactgccac agcaggatcc aagcctgatg ggcgtcaccg
    76621 taagcattaa tggtgccgat cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata
    76681 atcaaatcgt taccctgacc gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg
    76741 aaaaacagat gaccgcgcgt ggttacatgg ttggtccgaa tggcccggtt aatctgcaaa
    76801 tcatcgttag ccaactgtat gctgacgtgt cccagggcaa cgtgcgctac aacatcgcta
    76861 ccaaagcgga tatcgccatc atcgctaccg cgcagaatgg caacaaaatg accaaaaact
    76921 atcgtgccag ctacaacgtt gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg
    76981 cggttaacag tgtgctgacc gataccatcg ctgatatgtc tcaggacacc agcattcacg
    77041 aattcatcaa gcagaacgcg cgttaatttc tgccctctgg cccggtgcaa gccgggcctg
    77101 tagacgccca tgtccagtca atatttacgt atttttcaac agccgcgttc agccatattg
    77161 ctgatcctgg gttttgcttc cgggctaccg ctcgccctga catccggcac cttacaggcc
    77221 tggatgacgg tcgagaatat cgatctcaaa accattggtt tcttctctct ggtaggccag
    77281 gcttacgttt ttaaattcct ctggtcaccg ctgatggacc gctacacgcc cccatttttt
    77341 gggcggcggc gcggttggct gctcgccacg caaatcctgt tattagtcgc cattgcggcg
    77401 atgggttttc tcgaaccagg cacccaactc cgctggatgg cggcgctggc ggtggtgatc
    77461 gctttttgct ctgcctccca ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg
    77521 gcagaagaac gtggtgcggg cgcggcaatc agcgtgctgg gttaccgttt agggatgctg
    77581 gtttccggcg gcctagccct gtggctggca gataaatggt tgggctggca gggcatgtac
    77641 tggttgatgg cggcgctgtt gatcccctgt attatcgcga cgttgcttgc accagaacca
    77701 accgacacca ttcctgtgcc caaaacgctg gaacaagcgg ttgttgcacc tctgcgagat
    77761 ttctttggtc gcaataatgc ctggcttatt ttgcttctta tcgtgctgta taagctgggc
    77821 gacgcattcg ccatgagcct gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg
    77881 ggtgaagtag gcgtggtaaa caaaacgctt ggcttactgg cgaccattgt tggcgcattg
    77941 tacggtggga ttttgatgca gcgcctgtcg ctgttccggg cgctgctgat tttcggcatt
    78001 ttacaaggtg cgtctaacgc tggttactgg ctgctgtcga ttactgataa gcatctctac
    78061 agcatgggcg cagccgtctt tttcgaaaac ctctgtggcg ggatgggcac atcagccttt
    78121 gtcgcgctgt taatgacgct gtgtaataag tcattttccg ctactcaatt tgccctgctc
    78181 tcagcgcttt ccgctgtagg gcgagtttat gtcggccccg tggcgggttg gtttgttgaa
    78241 gcacacggct ggtcgacatt ctatctattc tccgtcgccg ctgccgtacc ggggcttatt
    78301 ttgctgctgg tttgccgcca gacgcttgaa tatacacgag taaatgacaa ctttatctcc
    78361 cgtaccgaat atccggcagg ttatgccttt gccatgtgga cactggcggc gggcgtcagc
    78421 ctgttggccg tgtggttact tctgttgacg atggacgcgc tggatttgac gcacttctct
    78481 ttcctgcctg ctctgctgga agtcggggtt ttagtcgccc tttctggcgt cgtgcttggt
    78541 ggtttgctgg attatctggc gctacgaaaa acgcatctga cgtaatctgt aaatattatt
    78601 tagctgtagt aatcactcgc cgtaattatt acggctaaat aaacatcagc atcgcttatt
    78661 aatatttgtt cttttgtgcg gcttagcgtt tggaaattat tggcgccatt tataaattct
    78721 atttttctta cacgattcag ctaatgagtc tttattttct caccacccag ttgtcactct
    78781 aatgataatt atttgttaaa taattgtttt atttcgcatt ggttatacca attgcccgcc
    78841 aagactcctt ctgcactccc cttttgttat aacgcccttt tgcaacagct tcttaaaatc
    78901 aacctgatat gttttgcaac atatgtgacc tgacagccaa atctaagtaa caggaattta
    78961 accatgttta cagtaatgta acctccccgt aaaatgccca cacactttaa acgccaccag
    79021 atcccgtgga attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg
    79081 ttgtcattat ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc
    79141 aaaggacaga ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg
    79201 attgtcgtta ttcccgcaat cttgatggct gttggtttcg cctggaagta ccgtgcgagc
    79261 aataaagatg ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc
    79321 tggacggtgc ctatcttaat catcatcttc cttgcggtac tgacctagaa aaccactcac
    79381 gctcttgagc ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt
    79441 tccatggact ggaaatggtt cttcatctac ccggaacagg gcattgcaac cgtgaatgaa
    79501 atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac
    79561 tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc
    79621 ctgcatctga tcgccaacga acccggtact tatgacggta tctccgccag ctacagcggg
    79681 ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgaattcgac
    79741 caatgggtcg caaaagcgaa acagtcgccg aacaccatgt ctgacatggc tgcgttcgaa
    79801 aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac
    79861 ttgtttgccg atgtgattaa caagtttatg gctcacggta agagcatgga catgacccag
    79921 ccagaaggtg agcacagtgc acacgaaggt atggaaggca tggacatgag ccacgcggaa
    79981 tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca
    80041 gtcccgttcc atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg
    80101 gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg
    80161 acctccgtcg accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg
    80221 ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggt
    80281 gaagcgggct tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt
    80341 atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg
    80401 cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttact
    80461 gttgttggtg tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc
    80521 tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg tgttgattac
    80581 tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc
    80641 gttaccattc tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc
    80701 tgggcatcac tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc
    80761 gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt
    80821 ggcaacatga tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc
    80881 ctgatcctgc ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt
    80941 ctgtttggtt atacctcgct ggtatgggca accgtctgca tcaccgtgct gtcgttcatc
    81001 gtttggctgc accacttctt tacgatgggt gcgagcgcga acgtaaacgc cttctttggt
    81061 atcaccacaa tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc
    81121 atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggactatcgg ttttatcgtc
    81181 accttctcgg tgggcggtat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt
    81241 ctgcataaca gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc
    81301 ttcggctgct tcgcagggat gacctactgg tggcctaaag cgttcggttt caaactgaac
    81361 gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg
    81421 ccgctgtatg cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg
    81481 cagttccaca ccatgctgat gattgcagcc agcggtgcag tactgattgc gctgggtatt
    81541 ctctgcctcg ttattcagat gtacgtttct attcgcgacc gcgaccagaa ctgtgacctg
    81601 actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc
    81661 tataactttg ccgtagtgcc gcacgttcac gaacgtgatg cattctggga aatgaaagag
    81721 aaaggcgaag cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc
    81781 ggtgcaggta tcgtcattgc agctttctcc accatcttcg gtttcgccat gatctggcat
    81841 atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc
    81901 ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag
    81961 catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc
    82021 gactgcccac gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt
    82081 ttggatctac ctgatgagcg actgcattct gttctcaatc ttgtttgcta cctatgccgt
    82141 tctggtgaac ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt
    82201 tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc
    82261 catgtacaaa aacaacaaaa gccaggttat ctcctggctg gcgttgacct ggttgtttgg
    82321 tgccggattt atcgggatga aaatctatga attccatcac ctgattgtta acggcatggg
    82381 tccggatcgc agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca
    82441 cgtcacttct ggtcttatct ggatggcggt gctgatggtg caaatcgccc gtcgcggcct
    82501 gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt
    82561 ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct
    82621 accgatcaca gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg
    82681 tcgatcattc tgacggtgat tccgttctgg atggtgatga caggggctgc ctctccggcc
    82741 gtaattctgg gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc
    82801 ttcctgcaca tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc
    82861 gtgctaatca tcgctatcct ggttgtaggc tccatctgga ttatgtggaa cctcaactac
    82921 aacatgatga tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga
    82981 aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg ctggcctcaa
    83041 agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg
    83101 cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga
    83161 cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca
    83221 ccttgctggg tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct
    83281 ggctgggggt gatgggcttt gtggtttatg tcggcgtcta tagcctgtac atgaaacgcc
    83341 actcagtcta cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgattggct
    83401 actgtgcagt aaccggcgag ttcgatagcg gcgcagcgat cctgctggct atcttcagcc
    83461 tgtggcagat gcctcactcc tatgccatcg ccattttccg ctttaaggat taccaggcgg
    83521 caaacattcc ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc
    83581 tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctgggt
    83641 ataaatatct ggtggtcgcc gcggcggtta gcgtctggtg gttaggtatg gctctgcgcg
    83701 gttataaagt tgctgatgac agaatctggg cgcgcaagct gttcggcttc tctatcatcg
    83761 ccatcactgc cctctcggtg atgatgtccg ttgattttat ggtaccggac tcgcatacgc
    83821 tgctggctgc tgtgtggtaa caaaacctct ctattaaaaa ggtgctacgg cacctttttt
    83881 attgtctgag atcagagata cggctaataa ccgtatctct tgacagagtg agcagatgca
    83941 cataaattaa tcatacggtt gatagccgta ttatgtatga gatcgactcc ctacgatttc
    84001 attcagttaa tcaaagaagt cataatgcct ggaaaaacag ccgctctata cgatgtcgat
    84061 aaaacgctga aaaatgcacg ggtcgaactc aaaactagtc ccgacgccaa aaacaaactt
    84121 cgtgaagctg cgcaagccgt tggggtagat ttaagcgctt ttattttgag cgttgctatg
    84181 gaacgcgccg aaagtgtgct tgataaccaa cgtcgccgtg agctttcgaa tcaaagctgg
    84241 gaactgatga accaactcat cgctgaacct gctcaaccga cgctcgccct caaggcgtta
    84301 atgaaaagga aaaacagcga tggtcgacaa gcatgaagag attactctgc ccatagtcct
    84361 ctcctgtaat tatcagtctg atattactta tcctgggcaa aaacagtttg attgcggtaa
    84421 ccctgttatc gataaatttg tacgcgcatc gctaaagaaa agtgtgcgta atagcgactg
    84481 tgcggctaaa gcacttattg acagacaaag tggtgaactg attggcatct gtacttttac
    84541 ggcatattcg ctggaaagac aacgcgtttc tggcgtcctt cagggttcac aaccttcaga
    84601 aattggtgtt gtcagattag tcatgttggg gggagcgcgg aagtatcaaa agcggggctt
    84661 tggtcaggac ctactatgtg
//
LOCUS       DADLSB010000003        64963 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.003, whole genome shotgun sequence.
ACCESSION   DADLSB010000003 DADLSB010000000
VERSION     DADLSB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.003"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="JN331_000180"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="JN331_000180"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY7258424.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JN331_000181"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JN331_000181"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY7258425.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JN331_000182"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JN331_000182"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY7258426.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="JN331_000183"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="JN331_000183"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY7258427.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="JN331_000184"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="JN331_000184"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY7258428.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDTLGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCCDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /gene="cptA"
                     /locus_tag="JN331_000185"
     CDS             7826..9559
                     /gene="cptA"
                     /locus_tag="JN331_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY7258429.1"
                     /translation="MHSTEVQAKPLFSWKTLGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQAMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="JN331_000186"
     CDS             9708..10559
                     /locus_tag="JN331_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7258430.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="JN331_000187"
     CDS             complement(10546..10887)
                     /locus_tag="JN331_000187"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7258431.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="JN331_000188"
     CDS             complement(10889..11767)
                     /locus_tag="JN331_000188"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY7258432.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="JN331_000189"
     CDS             complement(11733..14030)
                     /locus_tag="JN331_000189"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7258433.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNLRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="JN331_000190"
     CDS             complement(14081..14401)
                     /locus_tag="JN331_000190"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7258434.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15480)
                     /locus_tag="JN331_000191"
     CDS             complement(14416..15480)
                     /locus_tag="JN331_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY7258435.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAL
                     IAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGALTNSL
                     TQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAIAAVGI
                     CIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVIPSIMV
                     GSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVLKSLAR
                     KNGSSTDEKEDDLDLDFEIN"
     gene            15789..18290
                     /gene="ptsP"
                     /locus_tag="JN331_000192"
     CDS             15789..18290
                     /gene="ptsP"
                     /locus_tag="JN331_000192"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7258436.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSL
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18302..18964
                     /gene="fsa"
                     /locus_tag="JN331_000193"
     CDS             18302..18964
                     /gene="fsa"
                     /locus_tag="JN331_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7258437.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVITNPSIIAASKESIWEALP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            18975..19754
                     /locus_tag="JN331_000194"
     CDS             18975..19754
                     /locus_tag="JN331_000194"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HAY7258438.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEITPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTHAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGWRRLTRCITA"
     gene            19739..20022
                     /locus_tag="JN331_000195"
                     /pseudo
     CDS             19739..20022
                     /locus_tag="JN331_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005111035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
     gene            20411..20620
                     /locus_tag="JN331_000196"
     CDS             20411..20620
                     /locus_tag="JN331_000196"
                     /inference="COORDINATES: protein motif:HMM:NF018626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY7258439.1"
                     /translation="MADGARQQIGSTLFYDPAYIQLTYPGGDVPQERGVCSDVVIRAL
                     RSQKVDLQKLVHEDMAKNFAEYPQK"
     gene            20660..20914
                     /locus_tag="JN331_000197"
     CDS             20660..20914
                     /locus_tag="JN331_000197"
                     /inference="COORDINATES: protein motif:HMM:NF018626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY7258440.1"
                     /translation="MPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLAHIGVV
                     SDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20941..21879)
                     /gene="yijE"
                     /locus_tag="JN331_000198"
     CDS             complement(20941..21879)
                     /gene="yijE"
                     /locus_tag="JN331_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY7258441.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21940..24120)
                     /gene="katG"
                     /locus_tag="JN331_000199"
     CDS             complement(21940..24120)
                     /gene="katG"
                     /locus_tag="JN331_000199"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY7258442.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24449..25339)
                     /gene="metF"
                     /locus_tag="JN331_000200"
     CDS             complement(24449..25339)
                     /gene="metF"
                     /locus_tag="JN331_000200"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY7258443.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRSEMSYAICHTLGVRPGL"
     gene            complement(25688..28120)
                     /locus_tag="JN331_000201"
     CDS             complement(25688..28120)
                     /locus_tag="JN331_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY7258444.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28123..29283)
                     /gene="metB"
                     /locus_tag="JN331_000202"
     CDS             complement(28123..29283)
                     /gene="metB"
                     /locus_tag="JN331_000202"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY7258445.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEHALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29560..29877
                     /gene="metJ"
                     /locus_tag="JN331_000203"
     CDS             29560..29877
                     /gene="metJ"
                     /locus_tag="JN331_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY7258446.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30061..30669
                     /locus_tag="JN331_000204"
     CDS             30061..30669
                     /locus_tag="JN331_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY7258447.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30730..30942)
                     /gene="rpmE"
                     /locus_tag="JN331_000205"
     CDS             complement(30730..30942)
                     /gene="rpmE"
                     /locus_tag="JN331_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY7258448.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31145..33343
                     /gene="priA"
                     /locus_tag="JN331_000206"
     CDS             31145..33343
                     /gene="priA"
                     /locus_tag="JN331_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY7258449.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLHLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33499..34524
                     /gene="cytR"
                     /locus_tag="JN331_000207"
     CDS             33499..34524
                     /gene="cytR"
                     /locus_tag="JN331_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY7258450.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALCRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34616..35575
                     /gene="ftsN"
                     /locus_tag="JN331_000208"
     CDS             34616..35575
                     /gene="ftsN"
                     /locus_tag="JN331_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY7258451.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADTPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35668..36198
                     /gene="hslV"
                     /locus_tag="JN331_000209"
     CDS             35668..36198
                     /gene="hslV"
                     /locus_tag="JN331_000209"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY7258452.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36208..37539
                     /gene="hslU"
                     /locus_tag="JN331_000210"
     CDS             36208..37539
                     /gene="hslU"
                     /locus_tag="JN331_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY7258453.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLLPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKMKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37606..38532
                     /gene="menA"
                     /locus_tag="JN331_000211"
     CDS             37606..38532
                     /gene="menA"
                     /locus_tag="JN331_000211"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY7258454.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38625..39110
                     /gene="rraA"
                     /locus_tag="JN331_000212"
     CDS             38625..39110
                     /gene="rraA"
                     /locus_tag="JN331_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY7258455.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRILVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39195..39440)
                     /gene="zapB"
                     /locus_tag="JN331_000213"
     CDS             complement(39195..39440)
                     /gene="zapB"
                     /locus_tag="JN331_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY7258456.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39866..40711
                     /gene="glpF"
                     /locus_tag="JN331_000214"
     CDS             39866..40711
                     /gene="glpF"
                     /locus_tag="JN331_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HAY7258457.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPFDICVVEEKETTTPSE
                     QKASL"
     gene            40734..42242
                     /gene="glpK"
                     /locus_tag="JN331_000215"
     CDS             40734..42242
                     /gene="glpK"
                     /locus_tag="JN331_000215"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY7258458.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKTVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42472..43482
                     /gene="glpX"
                     /locus_tag="JN331_000216"
     CDS             42472..43482
                     /gene="glpX"
                     /locus_tag="JN331_000216"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7258459.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43579..44325
                     /gene="fpr"
                     /locus_tag="JN331_000217"
     CDS             43579..44325
                     /gene="fpr"
                     /locus_tag="JN331_000217"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY7258460.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44330..44758)
                     /gene="uspD"
                     /locus_tag="JN331_000218"
     CDS             complement(44330..44758)
                     /gene="uspD"
                     /locus_tag="JN331_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY7258461.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44785..45084)
                     /locus_tag="JN331_000219"
     CDS             complement(44785..45084)
                     /locus_tag="JN331_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY7258462.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45296..45736)
                     /locus_tag="JN331_000220"
     CDS             complement(45296..45736)
                     /locus_tag="JN331_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7258463.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            45837..46436
                     /locus_tag="JN331_000221"
     CDS             45837..46436
                     /locus_tag="JN331_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY7258464.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46544..47311
                     /gene="tpiA"
                     /locus_tag="JN331_000222"
     CDS             46544..47311
                     /gene="tpiA"
                     /locus_tag="JN331_000222"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY7258465.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47366..48121)
                     /gene="cdh"
                     /locus_tag="JN331_000223"
     CDS             complement(47366..48121)
                     /gene="cdh"
                     /locus_tag="JN331_000223"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY7258466.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48228..49217)
                     /locus_tag="JN331_000224"
     CDS             complement(48228..49217)
                     /locus_tag="JN331_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7258467.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49537..50499)
                     /gene="pfkA"
                     /locus_tag="JN331_000225"
     CDS             complement(49537..50499)
                     /gene="pfkA"
                     /locus_tag="JN331_000225"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY7258468.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50680..51582)
                     /gene="fieF"
                     /locus_tag="JN331_000226"
     CDS             complement(50680..51582)
                     /gene="fieF"
                     /locus_tag="JN331_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY7258469.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKILAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51731..52231)
                     /gene="cpxP"
                     /locus_tag="JN331_000227"
     CDS             complement(51731..52231)
                     /gene="cpxP"
                     /locus_tag="JN331_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY7258470.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHKQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52381..53079
                     /gene="cpxR"
                     /locus_tag="JN331_000228"
     CDS             52381..53079
                     /gene="cpxR"
                     /locus_tag="JN331_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY7258471.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53076..54449
                     /gene="cpxA"
                     /locus_tag="JN331_000229"
     CDS             53076..54449
                     /gene="cpxA"
                     /locus_tag="JN331_000229"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY7258472.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54555..55229)
                     /gene="yiiM"
                     /locus_tag="JN331_000230"
     CDS             complement(54555..55229)
                     /gene="yiiM"
                     /locus_tag="JN331_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY7258473.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIALGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55378..56361)
                     /gene="kdgT"
                     /locus_tag="JN331_000231"
     CDS             complement(55378..56361)
                     /gene="kdgT"
                     /locus_tag="JN331_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY7258474.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56621..57241)
                     /gene="sodA"
                     /locus_tag="JN331_000232"
     CDS             complement(56621..57241)
                     /gene="sodA"
                     /locus_tag="JN331_000232"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY7258475.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57526..58560
                     /gene="rhaT"
                     /locus_tag="JN331_000233"
                     /pseudo
     CDS             57526..58560
                     /gene="rhaT"
                     /locus_tag="JN331_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58557..59495)
                     /gene="rhaR"
                     /locus_tag="JN331_000234"
     CDS             complement(58557..59495)
                     /gene="rhaR"
                     /locus_tag="JN331_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY7258476.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(59479..60315)
                     /gene="rhaS"
                     /locus_tag="JN331_000235"
     CDS             complement(59479..60315)
                     /gene="rhaS"
                     /locus_tag="JN331_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY7258477.1"
                     /translation="MTVLHSVDFFPSCNASVAIELRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            60603..62072
                     /gene="rhaB"
                     /locus_tag="JN331_000236"
     CDS             60603..62072
                     /gene="rhaB"
                     /locus_tag="JN331_000236"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY7258478.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62069..63328
                     /gene="rhaA"
                     /locus_tag="JN331_000237"
     CDS             62069..63328
                     /gene="rhaA"
                     /locus_tag="JN331_000237"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY7258479.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            63570..64394
                     /gene="rhaD"
                     /locus_tag="JN331_000238"
     CDS             63570..64394
                     /gene="rhaD"
                     /locus_tag="JN331_000238"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY7258480.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFAVTPLASALAL"
     gene            64404..64718
                     /gene="rhaM"
                     /locus_tag="JN331_000239"
     CDS             64404..64718
                     /gene="rhaM"
                     /locus_tag="JN331_000239"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY7258481.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT        15822 a        17513 c        16630 g        14998 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcggga cactccagcg
     4081 atccagcacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgctgcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg tactgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatacgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgctgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttt
     7861 tagctggaaa accctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcaggc
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaaa tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cgctcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acgcccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc aggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccatga tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagctg ttaccaaccc aggcaccgat agcgcacgga gccagcgcag aacgctctgc
    15241 aatggagtaa ccaatgtaag cggcgaggaa aggcaccatc agcgtcaaac ctgcaacgcc
    15301 gatatcaaac agttttttca aattcggatc ggctacggca tccggcactg cgcctttgcc
    15361 atacaacatg acggaaaccg ccagcaaaat accgcccgat accacgaagg gaatcatgtg
    15421 tgaaacgccc gtcattaaat gctgacgggt atttttcagg atctgcacca actcattcat
    15481 aagctgctcc cgggcttgca aaagcccatg tccatatttt tgatggagga aacaataggc
    15541 aatcgcaggc ggagcaaaca gtggataaaa aggccattta ctggactttt gtaatatcag
    15601 tacaaaaatg cgatccgcct cataacttgc gataatccgg aagcatgccg caaaacggcg
    15661 tcttgtgaga cgcgtcgcaa attgacggtt acattacatt tgtccagctt ttggcagatt
    15721 tgtcacatgg caggccgtta ccgtgcgctc ttatcctgta aacagaaaac cgtaccggga
    15781 gaaaggcaat ggccctgatt gtggaattta tttgtgagct acctaacggc gtacatgcgc
    15841 gtccggcaag ccacgttgaa acgctgtgta atactttttc atctcaaatt gagtggcata
    15901 acctgcgtac tgaccgtaaa ggcaacgcca aaagcgctct ggcgctgatt ggcaccgata
    15961 cgctggtggg tgataactgc cagctactga tttccggggc cgacgaacag gaagggcacc
    16021 agcgtttaag ccaatggctg cgcgatgaat tcccccactg cgatgcgccg ctggcggaag
    16081 ttaaatctga cgaactggaa ccactgccgg tttcactgac caatctgaat ccgcaaatta
    16141 tccgcgcccg caccgtgtgc agcggtagtg caggcggcat tctgacgccg atctcttctt
    16201 tagatctcaa tgcgctgagt aatcttcccg cagccaaaga cgttgacgcc gagcaatccg
    16261 cactggaaaa cggcctgacg ctggtactga aaaacattga gtttcgtctg ctggatagcg
    16321 acggtgctac cagcgcgatt ctggaagcgc accgatccct ggctggcgat acttccctgc
    16381 acgaacattt actggcaggc gtcagcgccg gattaagctg cgccgaagcg attgtcgcca
    16441 gtgcgaatca cttttgcgaa gagttcgccc gttccagcag cagatacctg caagaacgtg
    16501 ccctagacgt acgcgacgtc tgcttccagt tactccagca aatctacggt gagcaacgct
    16561 tcccggcacc gggcaaactg acgcagtccg ccatttgtat ggctgatgaa ctgaccccca
    16621 gccagttcct cgaactggat aaaaatcacc tcaaaggatt gttactgaaa agcggcggca
    16681 ccacctcaca tacggtgatc cttgcccgtt cgttcaacat tccgacgctg gttggtgtgg
    16741 atattgatgc cctcactccg tggcagcatc aaacgattta tatcgacggc aacgccgggg
    16801 cgattgtggt tgagccagga gaagccgtag ctcgttatta tcagcaagaa gcccgcgtac
    16861 aggacgccct gcgtgagcaa cagcgtgtct ggctgaccca acaagcccgt accgctgacg
    16921 gtatccgcat tgaaattgcc gctaacatcg ctcactccgt ggaagcgcag gccgcattcg
    16981 gcaatggtgc ggaaggcgtt ggtttgttcc gcactgaaat gctctatatg gatcgcacca
    17041 gtgcaccggg cgaaagcgag ctgtacaaca ttttttgtca ggcgctggag tctgccaacg
    17101 gacgcagcat tattgtgcgc actatggaca ttggcggcga caaacccgtt gattatctaa
    17161 acattcccgc agaggcaaac ccgttcctcg gttatcgcgc cgtgcgtatt tatgaagaat
    17221 acgcgtcgtt atttaccaca caactacgct cgatcctgcg cgcctccgct cacggcagcc
    17281 tgaaaatcat gatcccgatg atctcctcac tggaagagat cttatgggtg aaagaaaaac
    17341 tggcagaagc caaacagcaa ctacgtaacg aacacattcc gtttgatgag aagatccagc
    17401 tcggcattat gctggaagtg ccgtcggtga tgttcatcat cgatcaatgc tgcgaagaga
    17461 ttgatttctt tagtattggt agtaatgacc tgacgcaata tctgctggca gtggatcgcg
    17521 ataacgctaa ggttactcgt cactacaaca gcctgaatcc ggcattcttg cgggcgctcg
    17581 attacgccgt gcaggcggtg catcgccagg gtaaatggat tggtctgtgc ggtgagctgg
    17641 gagcgaaagg ttccgtgctg ccgttgctgg tcggcttagg gctggatgaa ctcagcatga
    17701 gcgcaccatc aattccggcg gcgaaagcgc ggatggcgca acttgatagc cgtgagtgcc
    17761 gcaagttgct caaccaggca atggcctgcc gtacttcgct ggaagtggaa cacctgctgg
    17821 cgcaattccg catgacccaa caggacgcac cgctggtcac cgccgagtgc atcacgctgg
    17881 aaagtgactg gcgcagcaaa gaagaagtgc tcaaaggcat gaccgataac ctgctgctgg
    17941 cgggccgctg ccgctatccg cgtaaactgg aagccgactt atgggcgcgc gaggccgttt
    18001 tctctaccgg tctgggcttt agttttgcca ttccacacag caaatcagaa cacattgagc
    18061 aatccaccat cagcgtggcg cgtctgcaag cgccggtgcg ctggggcgat gatgaagcgc
    18121 aattcatcat tatgttaacc ctgaacaaac acgctgcggg cgatcagcac atgcgcattt
    18181 tctcgcgcct cgctcgccgc atcatgcacg aagaattccg taacgcgctg gttaacgccg
    18241 cctctgccga cgctatcgcc agcctgctgc aacatgaact ggaactgtaa aaggaaacat
    18301 catggaactg tatctggaca ccgctaacgt cgcagaagtc gaacgtctgg cacgcatatt
    18361 cccgattgcc ggggtgataa ctaacccgag cattatcgct gccagcaagg agtccatctg
    18421 ggaagcgctg ccgcgtctgc aaaaagcgat tggtgatgag ggcattctgt ttgctcagac
    18481 catgagccgc gacgcgcagg ggatggtgga agaagcgaag cgcctgcgcg acgctattcc
    18541 gggtattgtg gtgaaaatcc cggtgacttc cgaaggtctg gcagcaatta aaatactgaa
    18601 aaaagagggt attactacac ttggcaccgc tgtatatagc gccgcagaag ggttattagc
    18661 cgcactggca ggggcaaaat acgttgctcc gtatgttaac cgcgtagatg cccagggcgg
    18721 ggacggcatt cgtacggttc aggagctgca aacgctgtta gaaatgcacg cgccagaaag
    18781 catggtgctg gcagccagct ttaaaacgcc gcgtcaggcg ctggactgtt tactggcagg
    18841 atgtgaatcc atcaccctgc ccttagatgt agcgcaacaa atgctcaaca cccctgcggt
    18901 agagtcagct atagagaagt tcgaacacga ctggaatgcc gcatttggca ctactcatct
    18961 ctaaaggagc aattatggac cgcattattc aatcaccggg taaatacatc cagggcgctg
    19021 atgtgattaa tcgtctgggc gaatacctga agccgctggc agaacgctgg ttagtggtgg
    19081 gtgacaaatt tgttttaggt tttgctcaat ccactgtcga gaaaagcttt aaagatgctg
    19141 gactggtagt agaaattacg ccgtttggcg gtgaatgttc gcaaaatgag atcgaccgtc
    19201 tgcgtggcat cgcggagact gcgcagtgtg gcgcaattct cggtatcggt ggcggaaaaa
    19261 ccctcgatac tgccaaagca ctggcacatt tcatgggtgt tccggtagcg atcgcaccga
    19321 ctatcgcctc taccgatgca ccgtgcagcg cattgtctgt tatctacacc gatgagggtg
    19381 agtttgaccg ctatctgctg ttgccaaata acccgaatat ggtcattgtc gacaccaaaa
    19441 tcgtcgcggg cgcacctgca cgtctgttag cagcgggtat cggcgatgcg ctggcaacct
    19501 ggtttgaagc gcgtgcttgc tctcgtagcg gcgcgaccac catggcgggc ggcaagtgca
    19561 cccatgcggc gctggcactg gctgaactgt gctacaacac cctgctggaa gaaggcgaaa
    19621 aagcgatgct tgccgccgaa cagcatgtag taaccccggc gctggagcgc gtgattgaag
    19681 cgaacaccta tttgagcggc gttggttttg aaagcggcgg ctggcggcgg ctcacgcggt
    19741 gcataacggc ctgaccgcta tcccggacgc gcatcactat tatcacggtg aaaaagtggc
    19801 atttggtacg ctgacgcagc tggtactgga aaacgcgccg gtggaggaaa tcgaaaccgt
    19861 agctgcactt agccatgcgg taggtttgcc aataactctc gctcaactgg atattaaaga
    19921 agatgtcccg gcgaaaatgc ccatgcgacg ccagatcagg tttacgccgc actgctggta
    19981 gctgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    20041 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    20101 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    20161 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt ttatccgctt
    20221 ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    20281 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    20341 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatct aggccaatac
    20401 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcc
    20461 ggcgtatata cagcttacct atcctggcgg tgatgttccg caagaacgcg gtgtatgttc
    20521 cgatgtggtg atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga
    20581 agatatggcg aagaattttg ctgagtaccc gcaaaaatag aagttaaagc gcccggacag
    20641 caacatcgat caccgtcggg tgcctaatct ggaaacctgg tttagccgcc acaacaaaac
    20701 acgccccatc agtaaaaatg ccggtgacta tcaagcgggc gatattgtct cctggcgatt
    20761 agataacggg ctggcgcata ttggggtggt gtcagatggc ttcgcccgcg acggtacgcc
    20821 gctggtgata cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttagctggcg
    20881 gatggtgggg cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat
    20941 tcagatgctt tttacactga cagcttcttt tttcttacgg ctcaccagcg ccagtgccag
    21001 cacaatcagc acaataccgc taccttcaac ggcccccgga ttctcaccga gcagccacca
    21061 ggaaaagagt acgccacaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg
    21121 caagtttttc agcacaaaca gccataagct ccacgctagc gccgtcgcca gaatcgcact
    21181 gtaggccagc gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa
    21241 agcgaccaca ctcatcacca gcgccgcata cagcatctgc caggatgtta acgataataa
    21301 atccacgcgc ggatgacggg cgtataggcg tttagcaaca atcgcgctcg ccccccaact
    21361 aacaccggag aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg
    21421 ctgcaacacc aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg
    21481 caggcgttca ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct
    21541 caggatcgcc accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat
    21601 cccgcaggtt tgtaacaggg caatggctaa ggtgtattta aacggcgtcg ggcgcattcc
    21661 gcgaccacgt aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc
    21721 ggtaaagtcg aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact
    21781 ccagataagt gtgagcacaa ccaggccact gattgccagt gggttgctct ttcctgcggc
    21841 agacatgaga gatccagtgt gtagttttcc tgtggacact atacgccggt aaagaaactt
    21901 cagcgactgc ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt cgaaacggtc
    21961 gaggttcatc actttcaccc atgccgctac gaagtcttta acaaacttct cgtgggcatc
    22021 gctgctggcg taaacttccg ctactgcacg caggacagag ttagaaccaa ataccagatc
    22081 cgcacggctg gctgtgtatt tcacttcgcc ggtttcgcgg tcacgtcctt cgaacagctc
    22141 tttcgattcg tcagtcgctt tccactcgta acgcatatcc agcaagttca cgaagaagtc
    22201 attgctcaat acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc
    22261 acccagtaca cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg
    22321 ctgtgctttg tcgatcagca gtgactcggt ggtggaaacg tccagacgag cgcgatagtt
    22381 acggaaaccg tcagcaattg gctccagcag ctcaaacatc tcaatgtcag tctgatcctg
    22441 acgcgcatca acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc
    22501 ggctttctca acaccaacca caccagccag cacgatgata tccgccagcg aggctttacc
    22561 agactctttc tggattttct ccagaacagg cagagcacga acggctgcgg cgttcacatc
    22621 ccagtcgcgc tgcggcatta atgccagacg cgcaccgttg gcaccaccgc gtttgtcgcc
    22681 accacggaag gtagaagcag atgcccaggc caccgatacc agctcactaa cagacagacc
    22741 agaatccgca atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg
    22801 ctgcggcagc ggatcttgcc agatcagatc ttctttcggc acttccggcc cgatgtagcg
    22861 agatttcggc cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt
    22921 gaacgcctgc ggatcgttga ggaaacgacg agagatcttc tcgaactcag gatcaaaacg
    22981 cagcgtcagg tcggtcacca gcattgtcgg tttacgtttc ttcgacggat cgaacggatc
    23041 cgggataatt tccggtgcgt ctaccgcttc gaactggatt gctccagccg gactgcgggt
    23101 ctgtacccac tcatacttga acagattctc gaagaaatag ttgctccact gggtcggcgt
    23161 ctgggtccag actacttcca gaccagaggt aatggcatct gcgccaacgc cgctgccgta
    23221 agtgctcgcc cagcctaaac cttgttcttc aatcggtgca gcttctggat caggacctac
    23281 gtttgatgtc ggaccggcac cgtgggtttt acccagcgta tgaccacccg caatcagcgc
    23341 cacggtttct tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc
    23401 agaaagcggt tcgccgctgt gatccgggcc ttccgggtta acgtaaatca ggcccatctc
    23461 ggttgcaccc agcggtgctt tcgccagcgc ttccggatga cggtgagtca gccaggcttt
    23521 ttcatcaccc cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa
    23581 accgaaggta cggaagccgg agttttccag cgccacgtta cctgcgagga taaacaggtc
    23641 ggcccaggag attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt
    23701 atcgaggctt acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc
    23761 cgcgccgccg cgtccatcga ttgaacggta agtacctgcg ccgtgccaag ccatacgaat
    23821 aaacagaccg gcgtagctgc cccagtcggc tggccaccac ggctgagatt ctgtcagcag
    23881 ggctttcaga tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc
    23941 aaagtcctca cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag
    24001 ttgatttggc caccagtcgc gagtggttgt gcccgccccc gcgctctggt cgtgaccgcc
    24061 ctgatggaac gggcatttgc cagtggctgt ggtgttatgg atatcgtctg acgtgctcat
    24121 caatgtgctc ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa
    24181 ttgaattaat ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta
    24241 gatcaaattg atcgtaatta agcttttttg attatgtccg aattcggaca aatgctttat
    24301 aaaaaagggt tttagcttca tatccttcaa gtaatgaggg agaaaacaag gctgacaacc
    24361 ttattttccc gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac
    24421 tgtgtgattt tcacaaaagc cacactattt ataaaccagg tcgaacaccc agcgtatggc
    24481 aaatcgcgta actcatttca gagcggttaa gcgtatagaa gtggaaatct ttcactcctt
    24541 cacggcttaa aatcttcacc atatccatgg caatattcgc gcccaccagt ttgcgggttt
    24601 cggcatcgtc atccagcccg tcaaacattt gcgccatcca cgccggaata cgcacgttgg
    24661 tcatgtcggc aaatttcttc gcctgtttaa agttagatac cggcaaaatg cccgggatga
    24721 tttccacatc aatgcccgcc gatacacagc ggtcacgaaa acgcaggtag ctttcgacat
    24781 cgaagaagaa ctgagtaatc gcgcggttgg ctccggcatc cactttgcgt ttcagattaa
    24841 gcaaatccgc ctgagcgctt tttgcttccg ggtgaacttc cggatacgcc gccacggata
    24901 tatcgaaatc tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct
    24961 taccacttcc cggcggcaga tcgccacgca gtgccacgat atgacgaata ccgttattcc
    25021 agtagtcgcg tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg
    25081 gtgccgcttc cagaccagtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc
    25141 gctcgccgga gttcgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc
    25201 gatcgatgga gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaactcga
    25261 acgaaacgtt aatctgcccc tggacttctg ccaggctctg attcagggca tcccgctggc
    25321 tggcgtgaaa aaagctcata ccttacctca tcaatcgctt gtagttatat gttgtgttgt
    25381 gcacatctat ccatttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa
    25441 ccgaaaaatg accaattatg gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg
    25501 taagaaaaag aaaatccgat atgcaaagca aaccggactg caaatataaa ctaaccatgt
    25561 acttattgct tcttgccgga ttcgtcgtaa acgcttcatc cggcctaatt ctatgataat
    25621 tcaatatatt tcattagaaa ccatagaccc gaaaacatga gtaccgggca ttattaaatt
    25681 tctgaaatta caacaactgt gccagccggt tgatatccga ctgaatcgcc ccggcggtga
    25741 cgtcgcgccc agcaccaggc ccacggatca ccagcggatt atcgcggtac cagcggcttt
    25801 cgatggcaaa gacgttatcg catggcagca gtgatgccag cggatggtct tcacgcaccg
    25861 cctccacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtag cgcagcacca
    25921 gccccatttc gcgggccgct tccagccgtt gcaccatctg ctcgttaagt tcatcgccat
    25981 tttcaaagaa atggtcgatg ctgccgcctt cgcaatgagc aggcaccagc gattccacac
    26041 gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca
    26101 ttacgtcttt gccggagagg tcgtcacgcg gatcaggctc ggttaagccc tgctgccacg
    26161 cctgatccac cagctcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga
    26221 gcgtgccgga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc
    26281 gcacggtatg gttgatcggc aagcccgcac caacggtggc gttgtatagc cagtgacgcc
    26341 cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcccgcca
    26401 gtttgttggc actgatgacg tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa
    26461 gctgctggct ggcggtaacg tccaacacca ctaaatcatc atacggatgg gcgcgcatcc
    26521 acaggaaaag cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc
    26581 ggctggcgtc cagcccgtca taactcaaca gactgcggcg gctgtccacc acgcctgcca
    26641 gcacaaattc aaagccggta cgcgccgaaa gcgtgctctg ctcgcgggcg aacagttcca
    26701 gccagcgaga gccgatattg cctttgccga acaataccag gccgatgcgt ttttctgcgc
    26761 ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg
    26821 ccaccagact gatgccgtca tcggactgcc acgtaaactc gaccggctgg cctttcagtt
    26881 gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac acccgccccg accatcgcca
    26941 ccagcgccag cccctgacgc aggcgcagtt ctccaggtaa tcccgcttcg tcgaggattt
    27001 tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg cgatcgttat
    27061 gcacgccaac cgccagtgga cgcacctggg cacgtttcag gatctggtcg atctctttat
    27121 gcgccagttt gaaatcctga ctggcgggca cctgaaactc aatcaaacag acatcatcgt
    27181 ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac
    27241 cttgatccgg cgtgtagcta cagcgcagct gcaggtcgat ttcgctgcca gaaactggct
    27301 gtaaagtacg ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg gcctcatcca
    27361 gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggca ctgtataccc
    27421 cggcgacgtc gctccagatg gttacacgag aaacacccgc cagcgcaccg atttgtgtcg
    27481 cggaatagtc ggaaccgtta cgccccagca acaccgtttc accggcgttg ttacggctga
    27541 taaacccggt caccaccaga cgtttgctcg gatgttgtac cagtagctgt tgcagcaacg
    27601 ggtaagaaag cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcac
    27661 gggcatcaag ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg
    27721 ccgaccacac ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccactgt
    27781 cgagcaacgc cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ctatcggctt
    27841 cttcagcggg tagcagaccg ctaatcagat cgcactgata gcgacgcagc gtttgttgaa
    27901 cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag
    27961 tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagaac tccgccataa
    28021 tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca aatttatgca
    28081 gctgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc
    28141 ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat
    28201 acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg
    28261 tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt
    28321 aaacaacgac agcccgccca ggaaacgaca cagcgtctgc tcatcgccat ccagttcaaa
    28381 actcaacatt gcgccaaaac ctttttgctg acgcgcggca atttcatgcc cctgattttc
    28441 cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat
    28501 cgcctgcgcg ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgtag
    28561 cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag
    28621 ttcagtgaca acgtccgggt ctttagcaat caccacgcca gccaccacgt cagagtgacc
    28681 gttcagatat ttcgtacatg aatgcaacac cagatcggca cccaatgcca gcggattttg
    28741 taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag
    28801 atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag
    28861 taccagtttg ggtttttctg ccagcgctgc ccgtaatgca tgttcatcgc cttgatcaac
    28921 aaacaacacg cgatagcaac cgcgtttcgc cagactgtcg aacaggcgat agctaccgcc
    28981 gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag
    29041 gtgaatcgcg gacatgccgg tattagtaag tactgcgcca gcaccgcctt ccagttctgc
    29101 cagcgcacgc tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg
    29161 cggttcatta aatccggtaa agttataggt gctggaaaga tggatgggag ggacaacgca
    29221 accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt
    29281 catgtgatga agtttcctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt
    29341 gttaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc
    29401 ccaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac
    29461 ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag tacccacata aattgtgggg
    29521 tctggtctca atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa
    29581 tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt
    29641 tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac
    29701 aacctgcgtc acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc
    29761 gggcaacctt tgccagatga tgccgatctg cgtaaagagc gcagcgacga aatcccggaa
    29821 gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc
    29881 ggaaaagagc ggcgcggagt ggaatcgcct gatgcactac gcttatcagg cctacgtcat
    29941 attgcaattt attgaatttg cacgaacttg taggccggat aaggcgttca cgccgcatcc
    30001 ggcataaaca acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg acgtttaact
    30061 atgaaaaaca ggctgctgat cctcagcctg ctggtttctg tacctgcctt tgcctggcag
    30121 ccgcaaactg gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa
    30181 ctggcaaccc attccgatta cagccacacc ggtatgctgg tgatgcgtaa caaaaagccc
    30241 tacgtttttg aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat
    30301 ggtgaaaagg gcaactacgt tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag
    30361 caaaaactga cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt cagcttctca
    30421 tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg
    30481 ggaatgcgcg tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc
    30541 caggcgaagc tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca
    30601 ccacaagccg ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt
    30661 tcgtggtgat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
    30721 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
    30781 accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc
    30841 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
    30901 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
    30961 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
    31021 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
    31081 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
    31141 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct
    31201 gctgccagaa ggcatgacgg ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca
    31261 gcaggagcgc atcgggattg tggtatcagt tagcgatgcc agcgaactgc cgctcactga
    31321 gctaaaagcg gtagtcgaag tgctggatag tgagccggtg tttactcact ccgtctggcg
    31381 attgctgcta tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc
    31441 cttgccgatt ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt
    31501 tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca
    31561 acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga
    31621 atttaatgat gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga
    31681 aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt
    31741 gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc
    31801 ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga
    31861 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc
    31921 gcaaactatc gcccgttttc gtgaacggtt taatgcgccc gtggaagttc tgcattccgg
    31981 tctgaacgac agcgagcgtc tttcggcgtg gctgaaagcg aaaaatggcg aggcggcgat
    32041 tgtgatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat
    32101 tgacgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga
    32161 cctggcggtg tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc
    32221 cgcgctggaa acgttgtgca acgtccagca gaaaaaatac cgcctgctgc acctgacgcg
    32281 ccgggcgggg aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt
    32341 gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa
    32401 tcaggttatc ctctttctta accgccgtgg ctttgcgcct gcgctactgt gccacgactg
    32461 cggctggatt gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca
    32521 gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg
    32581 cggttccacg cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgcttgc
    32641 gccgctgttc cctgatgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
    32701 ggcgctggaa cagcaactgg cggaagtaca tcgcggcggt gcgaggattt tgatcggcac
    32761 acaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt
    32821 ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac
    32881 ccaggtcgct ggtcgtgccg ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca
    32941 ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg cctttgccga
    33001 acaggcgctg gctgagcgac ggatgatgca actgccgcca tggaccagcc atgtgattgt
    33061 gcgtgcggaa gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct
    33121 gatcctctcc agcccgctgg cagacgacaa actgtgggtt ctcggtccgg ttccggcact
    33181 ggcacctaaa cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt
    33241 gcgcttgcaa cacatcatta gtggtacgct ggcgctcatc aatacaatac cggattcccg
    33301 caaggtgaaa tgggtactgg atgttgatcc gattgagggt taaaccgctt acgatgcgag
    33361 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
    33421 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
    33481 gccaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
    33541 gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat
    33601 aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta
    33661 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc
    33721 ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
    33781 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
    33841 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg
    33901 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
    33961 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
    34021 gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa
    34081 gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgtg tcgctgcgga
    34141 attatggttg atccacaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa
    34201 gcaatgcaac agctgcttga tcttccacaa ccgcctactg cagtcttctg ccatagcgat
    34261 gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac
    34321 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
    34381 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
    34441 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
    34501 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
    34561 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
    34621 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
    34681 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
    34741 cgttcttgtg acctttatcg gaggtctgta cttcattacg catcacaaga aagaagagtc
    34801 cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
    34861 ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc
    34921 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct
    34981 tgaacaaatg caagctgata tgcgccagca gccaacgcag ctggttgagg ttccgtggaa
    35041 tgaacagacg ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt
    35101 agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
    35161 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccagcttc
    35221 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
    35281 gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacacg ccaaaaccga cggcggagaa
    35341 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
    35401 gacggtacgt gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg
    35461 ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
    35521 tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
    35581 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
    35641 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
    35701 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
    35761 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
    35821 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
    35881 ctggttaaag ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa
    35941 cttgaagcac tgctggcagt cgcggatgaa actgcatcgc ttatcatcac cggtaacggt
    36001 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
    36061 gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag
    36121 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
    36181 ttaagctaca aagcataagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
    36241 gaactggata agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg
    36301 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
    36361 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct
    36421 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aatttaccga agtgggctac
    36481 gtcggtaagg aagttgattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
    36541 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag agctggcaga agaacgtatt
    36601 ctcgacgtgc tgctcccacc tgctaaaaac aactggggac agactgaaca gcagcaggaa
    36661 ccttccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
    36721 gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc
    36781 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa
    36841 aaagcgcgta agatgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
    36901 aaactggtga acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg
    36961 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
    37021 gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc
    37081 accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
    37141 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc ggctgccaat ccgcgttgaa
    37201 ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc
    37261 accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc
    37321 ggtattaaac gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
    37381 gctcgtcgtt tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc
    37441 gatttaagcg gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg
    37501 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
    37561 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
    37621 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
    37681 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccgctggtc
    37741 gccctgctgg cactaattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    37801 tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg
    37861 caaaaagggg tcattaccca gcaaaagatg aaacgggcgc tcattattac cgtcgtgctc
    37921 atctgtctct ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc
    37981 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    38041 cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg
    38101 agtgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    38161 gcgaccgcat gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc
    38221 aatagcgacc gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg
    38281 cgtcgttatc atgcctgcct gctgatgggc tcgctggtgt gtctggcgct gtttaatctc
    38341 ttttcgctgc atagcctgtg gggctggctg ttcctgctgg cggcaccatt actggtgaag
    38401 caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt
    38461 actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag
    38521 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata
    38581 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa tcctatgaaa tacgatactt
    38641 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    38701 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    38761 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tattcttgtc gtcgatggcg
    38821 gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    38881 aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg
    38941 atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg
    39001 aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg
    39061 ccgacaatac cgggattatt ctttcagaag atccgctgga tattgaatga taagaaaggc
    39121 accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga
    39181 aagatgaagc gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc
    39241 cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc
    39301 tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc
    39361 atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttctcaaac
    39421 acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc
    39481 tcgtcccgat taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg
    39541 tatgaagccg cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt
    39601 ttcatccttc tcctagtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg
    39661 cgaatgatct aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc
    39721 tcattttatg acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac
    39781 gataagttaa cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca
    39841 aacattaact cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat
    39901 tgctgaattc ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact
    39961 aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt
    40021 ggcaatggcc atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac
    40081 cattgcattg tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc
    40141 acaagttgcc ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt
    40201 tttcgacttc gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc
    40261 tggcactttc tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga
    40321 gatggtgatt accgctattc tgatggggct gatcctggcg ttaacggacg atggcaacgg
    40381 tgtaccacgc ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc
    40441 atctatgggc ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt
    40501 ctttgcctgg ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta
    40561 cttcctggtg ccgcttttcg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg
    40621 caaactgatt ggtcgccatt tgcctttcga tatctgtgtt gtggaagaaa aggaaaccac
    40681 aactccttca gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg
    40741 aaaaaaaata tatcgttgcg ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg
    40801 atcacgatgc caatatcatt agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac
    40861 caggttgggt agaacacgac ccaatggaaa tctgggccac ccaaagctcc acgctggtag
    40921 aagtgctggc gaaagccgat atcagttccg atcaaattgc agctatcggt attacgaacc
    40981 agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa gcctatctat aacgccattg
    41041 tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt aaaacgtgac ggtttagaag
    41101 attatatccg cagcaatacc ggtctggtga ttgacccgta cttttctggc accaaagtga
    41161 agtggatcct cgaccatgtg gaaggctctc gcgagcgtgc acgtcgtggt gaattgctgt
    41221 ttggtacggt tgatacgtgg cttatctgga aaatgactca gggccgtgtc catgtgaccg
    41281 attacaccaa cgcctctcgt accatgttgt tcaacatcca taccctggac tgggacgaca
    41341 aaatgctgga agtgctggat attccgcgcg agatgctgcc agaagtgcgt cgttcttccg
    41401 aagtatacgg tcagactaac attggcggca aaggcggcac gcgtattcca atctccggga
    41461 tcgccggtga ccagcaggcc gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga
    41521 agaacaccta tggcactggc tgctttatgc tgatgaacac tggcgagaaa acggtgaaat
    41581 cagaaaacgg cctgctgacc accatcgcct gcggcccgac tggcgaagtg aactatgcgt
    41641 tggaaggtgc ggtgtttatg gcaggcgcat ccattcagtg gctgcgcgat gagatgaagt
    41701 tgattaacga cgcctacgat tccgaatatt tcgccaccaa agtgcaaaac accaatggtg
    41761 tgtatgtggt tccggcattt accgggctgg gtgcgccgta ctgggacccg tatgcgcgcg
    41821 gggcgatttt cggtctgact cgtggggtga acgctaacca cattatacgc gcgacgctgg
    41881 agtctattgc ttatcagacg cgtgacgtgc tggaagcgat gcaggccgac tctggtatcc
    41941 gtctgcacgc cctgcgcgtg gatggtggcg cagtagcaaa caatttcctg atgcagttcc
    42001 agtccgatat tctcggcacc cgcgttgagc gcccggaagt gcgcgaagtc accgcattgg
    42061 gtgcggccta tctcgcaggc ctggcggttg gcttctggca gaacctcgac gagctgcaag
    42121 agaaagcggt gattgagcgc gagttccgtc caggcatcga aaccactgag cgtaattacc
    42181 gttacgcagg ctggaaaaaa gcggtcaaac gcgcgatggc gtgggaagaa cacgacgagt
    42241 aatgtcaggc acctgaatcg attgcctgat gctgatcgcc tgatgcgacg cttacgcgtc
    42301 ttatcaggcc tacgagccaa tgccattttg tgtaggcggg ataaggcgtt cacgccgcat
    42361 ccggcatcac gcagactctg aaaccgtgcg ggggcaaccc cgcacacatc aataatccca
    42421 cccttctcct gtgctacact tcgcgccatt ccttactgct tagagtttgc tatgagacga
    42481 gaacttgcca tcgaattttc ccgcgtcacc gaatctgcgg cgctggctgg ctacaaatgg
    42541 ttaggacgcg gcgataaaaa caccgcggac ggcgcggcgg taaacgccat gcgtattatg
    42601 ctcaaccagg tcaacattga cggcaccatc gtcattggtg aaggtgaaat cgacgaagca
    42661 ccgatgctct acattggtga aaaagtcggt actggtcgcg gcgacgcggt agatattgct
    42721 gttgatccga ttgaaggcac gcgcatgacg gcgatgggcc aggctaacgc gctggcggtg
    42781 ctggcagtag gcgataaagg ctgcttcctc aatgcgccgg atatgtatat ggagaagctg
    42841 attgtcgggc cgggagccaa aggcaccatt gatctgaacc tgccgctggc ggataacctg
    42901 cgcaatgtag cggcggcgct cggcaaaccg ttgagcgaac tgacggtaac gattctggct
    42961 aaaccacgcc acgatgccgt tatcgctgaa atgcagcaac tcggcgtacg cgtatttgct
    43021 attccggacg gcgacgttgc ggcctcaatt ctcacctgta tgccagacag cgaagttgac
    43081 gtgctgtacg gtattggtgg cgcgccggaa ggcgtggttt ctgcggcggt gatccgcgca
    43141 ttagatggcg acatgaacgg tcgtctgctg gcgcgtcatg acgtcaaagg cgacaacgaa
    43201 gagaatcgtc gcattggcga gcaggagctg gcacgctgca aagcgatggg catcgaagcc
    43261 ggtaaagtat tgcgtctggg cgatatggcg cgcagcgata acgtcatctt ctctgccacc
    43321 ggtattacca aaggcgatct gctggaaggt attagccgca aaggcaatat cgcgactacc
    43381 gaaacgctgc tgatccgcgg caagtcacgc accattcgcc gcattcagtc catccactat
    43441 ctggatcgca aagacccgga aatgcaggtg cacattctct gagtgatttg atcgattgag
    43501 ccttccagtc cttcgggact ggaatttttt tgttcggaga acgaagataa ggcaagtcaa
    43561 tcaaaacagg agaaaaacat ggctgattgg gtaacaggca aagtcactaa agtgcagaac
    43621 tggaccgacg ccctgtttag tctcaccgtt cacgcccccg tgcttccgtt taccgccggg
    43681 caatttacca agcttggcct tgaaatcgac ggcgaacgcg tccagcgcgc ctactcctat
    43741 gttaactcgc ccgataatcc cgatctggag ttttacctgg tcactgtccc cgatggcaaa
    43801 ttaagcccac gattggcggc actgaaaccg ggcgatgaag tgcaggtggt tagcgaagca
    43861 gctggcttct ttgttctgga tgaagtaccg gactgcgaaa cgctatggat gctggcaacc
    43921 ggtacagcga ttggccctta tttatcgatt ctgcaactag gcaaagattt agatcgcttc
    43981 aaaaatctgg tcctggttca cgccgcacgt tatgccgccg acttaagcta tttgccactg
    44041 atgcaagaac tggaaaaacg ctacgaaggc aaactgcgca ttcagacggt ggtcagtcgg
    44101 gaaacggcag cggggtcgct cacgggacgg ataccggcat taattgaaag tggggaactg
    44161 gaaagcgcga ttggcctgcc gatgaataaa gaaaccagcc atgtgatgct gtgcggcaat
    44221 ccacagatgg tgcgcgatac acaacagttg ctgaaagaga cccggcagat gacgaaacat
    44281 ttacgtcgcc gaccgggcca tatgacagcg gagcattact ggtaagcggt tacttatcga
    44341 taaacggcac gatgagcaaa tccgcactca tcttattgat catcccgcga tatgccggca
    44401 tcaaacggtt gataaatgag tgatgatgac cacagacaag gaggtcgcac tgctcttttt
    44461 gcataatttc cagcagtgtt tccggcattt ctccgcgttc aatacgcagt tttgtcttcg
    44521 gccattgaat atttttcgtc agtttataca gcttgttatc cgacttattc ttcaacaatt
    44581 gaagaatatc ttctgttgca gggaagtaga tacccggata caactcgctt aagccatcat
    44641 caatatgaat taacgtcagg tgagcgtcat tatgtctggc gagctccagg gctttattca
    44701 ccagtaaggc atcttcttca ttcccggaaa ttgccacgcc aatgtgttta taagccatgt
    44761 ttaactcctt ctaaagcaac tccatcaagc taacgaacgc agtgataact caaaaataat
    44821 catctcggcc tggcatgaga aagtgaaggc cgcatcaagc tcaacttgcc cctccagatc
    44881 cttaatctta taggccacgc tggccccttc atctgcagag gatgcagcgg cggcaagctc
    44941 tttcagtttg gtcataaaag actctgcttc ttcgcgggtg gcaaaaaagc gcgaaaactt
    45001 actggtacag ttgtcgttat caatcaccgt accgatatca atcgcacatc ctttagtact
    45061 gcatttatct acgacatctt tcatagggca cctctgtata ttcgcctctc tgtcgattac
    45121 gacttaagaa taatgcgccg ttaagggaag aacaaacaag taatgatctt atggattacc
    45181 attttttttg atattgctta aggtgaagag ttcagaagga ttttccgggt attgtcattt
    45241 gcatcgtgga aataacaatc ccggaaaaaa atagcagtaa gagatatatt aaaggttaat
    45301 tacttgattt attgtcggct ttatacttca catcctgagt atctttacca tatttatttt
    45361 cgccttgtgt gccaacaaac gcaccaagat cgataagcat catcaccaga atcaacgtcg
    45421 ggacaaaacg ccccaccgcc cattgccaga cacccggtaa aatcgcccag ttacccgcca
    45481 gcagcatcca cgccacaatc atcagaaatg cccatgcgcc ggaacgcccg cgatcatgca
    45541 agcgcttaac agttactgcc gctgttggcc agagcaagca cacaaggcaa aacgccgcgg
    45601 tctgaatatc gagtagattc ttacccgcca gtgagaaaag caccagcatg cctgcgaacc
    45661 acaggcctat ccaaatccag aaatcacggc gtccaatacg ccctttaaat gagaataacc
    45721 attgctgtat ggtcatgtaa gttccttgat ggttgtcttt tcctggattc tacccttttg
    45781 acaagggcga cagatatcgt tttaatcgga gccagtcata acaaaaggta ctgtcaatga
    45841 agccagggtg tacgctgttt tttctcttat gttctgcatt aaccgttaca acaacggcgc
    45901 atgcacaaac accagatacg gcaacgaccg cgccttatct gctggctgga gcccctactt
    45961 tcgatctctc catcagccag tttcgagaag actttaacag ccagaatccc agcctgccac
    46021 tgaacgaatt tcgtgccatc gacagcagtc ccgacaaagc caatctcact cgtgctgcaa
    46081 gtaaaattaa cgagaacttg tatgcttcta cagcgctgga gcgcggtacc ttaaaaatca
    46141 aaagcattca aatgacctgg ctacccatcc aggggccaga gcaaaaagca gcgaaagcga
    46201 aagctcagga atacatggca gcagtgatcc gcacactcac cccattaatg accaaaacac
    46261 aaagccagaa aaaactgcag tcgctactaa cggcggggaa aaacaaacgt tattacaccg
    46321 agacagaagg tgcactgcgt tatgttgtcg cggacaacgg cgaaaagggg ctgaccttcg
    46381 ctgttgaacc gattaagctg gcgctatctg aatcgcttga aggtttgaat aaatgacaaa
    46441 aagcaaagcc tttgtgccga tgaatctcta tactgtttca cagacctgct gccctgcggg
    46501 gcggccatct tcctttattc gcttataagc gtggagaatt aaaatgcgac atcctttagt
    46561 gatgggtaac tggaaactga acggcagccg ccacatggtt cacgagctgg tttctaacct
    46621 gcgtaaagag ctggcaggtg ttgctggctg tgcggttgca atcgcaccac cggaaatgta
    46681 catcgatatg gcgaagcgcg aagctgaagg cagccacatc atgctgggtg cgcaaaacgt
    46741 ggacctgaac ctgtccggcg cattcaccgg tgaaacttct gccgctatgc tgaaagacat
    46801 cggcgctcag tacatcatca tcggccactc tgaacgtcgt acttaccaca aagagtctga
    46861 cgaactgatt gcgaaaaaat tcgcggtgct gaaagagcag ggtctgactc cggttctgtg
    46921 catcggtgaa accgaagctg aaaacgaagc gggaaaaact gaagaagttt gcgcacgtca
    46981 gatcgacgcg gtactgaaaa ctcagggtgc tgcggcattc gaaggtgcgg ttatcgctta
    47041 cgaacctgta tgggcaatcg gtactggcaa atctgcaact ccggctcagg cacaggctgt
    47101 tcacaaattc atccgtgacc acatcgctaa agttgacgct aacatcgctg aacaagtgat
    47161 cattcagtac ggcggctctg taaacgcgtc taacgctgca gaactgtttg ctcagccaga
    47221 catcgacggc gcgctggttg gcggtgcttc tctgaaagct gacgctttcg cagtgatcgt
    47281 taaagctgca gaagcggcta aacaggctta agtctgacag gtgccggatt tcatatccgg
    47341 cacttacttt ccttaactcc tcgccttaac gcaaaatctc acactcatga tcctggattt
    47401 cctcggctga agcacggttc agcgtcagta gatttcgttg tgtcgccagc aacacaaacg
    47461 aattatcact ctgccgtacc atcgccagcc cgtagcttcc catatgttcc cgcgcctcag
    47521 gcacttcttc tgcaagcatc ataaatgggc tgcgttgtac cagttcgctt tccgttaccc
    47581 gacgcgccaa gtattcatgc ccgcgcaaac cacctggcag tggcaaccag cggctgctga
    47641 tgttcgccag atttttatcc agttgttcgc gcacatcagg acgaatacaa gagatatgaa
    47701 tatgaaaatg gttttgcgta cgcccggtgc gggagttgat cgccaaagaa accgcgcgat
    47761 cgggaaccgg ctggccgtat tttttgctca taaaatcacg cgcctgccag gccaaccaaa
    47821 agaagttcgg cgttgaagga tcggtcaaca aaggactttc agtaccgtta atacgatacg
    47881 ttggcatcaa cagatattgc agtggaccat taagatcttt taaaaccacg tatccggcat
    47941 tgggtttgac ttccgcacat ggcgaaggat tttgattttg ctgctgattg ggcaaacatt
    48001 cctcaaggac aatcttacgt aatgtatccg actcttcacc ggttaatttc cagtaaccaa
    48061 taccggcagc cacaacggcg ataactatca tcaccaaaaa aagaagaccc gcttttttca
    48121 tctttttttc cctgtacctc aaagagatgc aagggtaacg caaaatcgtg acaaataaaa
    48181 aacccggtcg cgagaaacca ccgggtttga taattatcct agggaaatca gcgtttgctg
    48241 atctgatcga acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg
    48301 aactcttcat caatggtgaa taacttcagc tttggaaacg cattttcgta ctttttcgct
    48361 acttcagcgt cgcgcggacg gtagtagttt ttcgcggcaa tttcctgacc ttctggcgag
    48421 tagagatatt tcaggtaggc ttccgccacc tctttggtgc cttttttctc gaccacttta
    48481 tcgaccaccg acaccgttgg ctctgcgagg atagactcac tcggcgtgac gatttcgaat
    48541 ttatctttcc ccagttcatt cgctgccagc agagcttcgt tttcccaggc gatcagtaca
    48601 tcgccaatcc cgcgctcgac aaaggtgtta gtggagccgc gcgcgccaga atccagaact
    48661 tcgacgtttt tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg
    48721 tgatgcagcg cgtagcccca ggcagccagg tagttccagc gtgcgccacc agagcttttc
    48781 ggattaggcg tgatcaccga aacacccggt ttaatcagat cgttccagtc atggatctgc
    48841 ttcggattgc ccttacgcac caggaaaaca atggtggaag tgtacggtgc ggagttatcc
    48901 ggcagacgtt tgatccactc tttatcaatc cgcccgcgtt ccgcaatcgc gtccacgtca
    48961 taggccagag ccagcgtgac aacatcagct tcaataccgt tgattaccga cgtcgcttgt
    49021 ttacctgaac caccgtgtga ctgacgaatc accacgttat caccagtttg ctgtttccag
    49081 tgggcgctga atgccttgtt gtactgttcg tacaattcgc gcgttggatc atatgaaacg
    49141 ttaagaagct gaatatcctt tgccataacg ctggttgccg ccagcaaaaa tgttaaccct
    49201 acgccccact tgttcatcgc ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc
    49261 agaaggttaa ccaaacatta aagaataaaa aaagattggc tataacttgc gggtatatgt
    49321 tgagggatta aaaaggcgga agaaatccgc ctcatattgc tgacaaagtg cgctttgtcc
    49381 atgccggatg cggcgcgaac gccttatccg gcctacaaaa gtttgcaaat tcaataaatt
    49441 gcagaattca tgtaggcctg ataagcgaag cgcatcaggc atttttgctt ctgtcatcgg
    49501 tttcaggcta aaggaatctg cctttttccg aaatcattaa tacagttttt tcgcgcagtc
    49561 cagccagtca cctttgaacg gacgcttcat gttttcgata gcgtcgatga tgtcgtggtg
    49621 aaccagctgt tcgttctgga tacctacaca acgaccgccg taacctgcca acagcagatc
    49681 gatagcgtaa gcgcccatac gggaagccag aatacggtcg taaggcaccg gagaaccacc
    49741 gcgctggatg tggcccagca cagttgcgcg ggtttcacga ccggtttctt tctcgatgaa
    49801 atgcgccagt tcgtcaacat cacacatatg ttcggtaatc gccacgatcg cgtgtttttt
    49861 acctttcgcg atacccgctt tgatttcgtt taccaggtct tcacggctga attcaacttc
    49921 cggaacgaca acgaattcac agccaccggc aatggccgca gccaaggtca gatcgccaca
    49981 ataacggccc atcacttcca ccacggaaat acgctggtga gaagaagagg tgtcacgcag
    50041 acggtcgatc gcttctacaa cggtgctcag cgcagtgaag aaaccgatag tgtagtcagt
    50101 gcctttgatg tcgttgtcga tagtgcccgg cagaccgatg cacgggaagc ccatttcggt
    50161 cagacgcatt gcacccatgt aggaaccgtc accgccgata accaccagcg cgtcgatacc
    50221 acgttttttc aggttttcga tagccacggc gcggatgttc tcgtcgcgga attccgggaa
    50281 acgcgcagaa ccgaggaacg taccgccacg gttgatcatg tcagacacgc tgtaacggtc
    50341 tagctgtacc atacggtctt catacagacc cagatagccg tcataaatac ccattacttc
    50401 cagaccttct gtcagcgcag aacgaacaac cccgcgaatt gcggcgttca tgcctggcgc
    50461 atcaccgccg cttgtcaaca cacctatttt cttaatcatg actacctctg aactttggaa
    50521 tgcaaaatga aatctgttgc cggaagtctt cttgcacatc gaagtgatcc aacgaatgtg
    50581 caaatagtat aacaatcact tcctgctgaa ttgattcagg tcaggccaaa tggcggtatt
    50641 ttatacacaa aatgcgggtc tggctctctt ttatactgat tatgaaagca tagaccgttt
    50701 accctccctg ggtacgacgg aacaggggtc ctgatggata atcacatccg atcccggaaa
    50761 acgccgtaaa atagcctgct ctacctgatc cgccaccata tgtgcctgaa ccaaaggcag
    50821 agagtcttcc atttccaaat gaatctgaat aaagcgggtc ggccctgact gccgcgtgcg
    50881 aagatcgtga gcgccgctaa cacccggcca ggaagtcacg atatcaataa tttcttgccg
    50941 ttcttcatca ggcaatgcgc gatccagtaa tgactgtacc gcctcatatc ccatgcgtaa
    51001 cgcgctatat aaaatataga tgccgattcc caatgcaaac agagcatcgg cgcgatgcca
    51061 gccgtaccaa gacaacccca gcgccagcag aattgcgccg ttcatcataa catcagactg
    51121 gtaatgtagc atatcagccc gcaccgcctg gctttgcgtc cggcgcacca cccaccgctg
    51181 aaacgagaca aggataatcg tacaaattag cgccacaatt gtcacgataa ccccgacgcc
    51241 tggatctgtc atcggtgttg gagataccag atgttgaata cccgtcaaaa acaggaatag
    51301 tgccgaaccg gagataaaca tactttgcgc cagcgccgcg agggactcag ctttaccgtg
    51361 accaaacgag tgattatcgt cggcaggttg cagggaatat cgcaccacca gtaaattcgt
    51421 caacgacgcg ccgatatcca ccagcgaatc caccagcgcg gcgagaatac tcaccgaccc
    51481 ggtataccac catgccaaaa ttttaatcag caatagcaac gaagccatcg ccgtcgcagc
    51541 aatcgccgcc cgactgacca gccgtccata agattgattc ataaatactc ccgctatcaa
    51601 ctgacgctag tataacggaa gcaaatcatc tgcaatgcat taagcagcag gcaaattgag
    51661 gataaaaaaa aaccccacat catgtggggg aagacaggga tggtgtctat ggcaaggaaa
    51721 acagggttta ctactgggaa cgtgagttgc tactactcaa tagcttcaac gatgaacttt
    51781 tttgccattg cgtcacgtca cgcaactgct ccattcgttg ttgatgtttc tcgtttaaaa
    51841 ccgcttgctg ctccggcgtt aacaggcgat acatttggtt gcggactttt gccatctcaa
    51901 cctgacgagc aatttgctca ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat
    51961 caaaattttc tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca ttaacaggag
    52021 gctgtttgtg ccgggcctgt tgcataagat ctcgcatctg ctgacgctga tgttcggtta
    52081 aacttatgcc gtcgaacata tggctctgcg tactgcgctg cgtaagttct tcacccggat
    52141 gccagttatc gcctgaaccg acttcagcag cgtggcttaa tgaactgact gccagcgttg
    52201 aggccatgac ggcagcggta actatgcgca tcatttgctc ccaaaatctt tctgtcgcga
    52261 ttcaacgata gagagtttac gattcaggct gcaaacatgc gtcagggggt gtaaaacaac
    52321 gtaaagtcat ggattagcga cgtctgatga cgtaatttct gcctcggagg tatttaaaca
    52381 atgaataaaa tcctgttagt tgatgatgac cgagagctga cttccctatt aaaggagctg
    52441 ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc gcttgatctt
    52501 ctggacgaca gcattgattt acttttgctt gacgtaatga tgccgaagaa aaatggtatc
    52561 gacacattaa aagcacttcg ccagacacac cagacgcctg tcattatgtt gacggcgcgc
    52621 ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg cagatgacta tctcccgaaa
    52681 ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg
    52741 agcgagcaac agcaaaacaa cgacaacggt tcaccgacac tggaagttga tgccttagtg
    52801 ctgaatccag gccgtcagga agccagcttc gacgggcaaa cgctggagtt aaccggtact
    52861 gagtttaccc tgctctattt gctggcacag catctgggtc aggtggtttc ccgtgaacat
    52921 ttaagccagg aagtgttggg caaacgcctg acgcctttcg accgcgctat tgatatgcac
    52981 atttccaacc tgcgtcgtaa actgccggat cgtaaagatg gtcacccgtg gtttaaaacc
    53041 ttgcgtggtc gcggctatct gatggtttct gcttcatgat aggcagctta accgcgcgca
    53101 tcttcgccat cttctggctg acgctggcgc tggtgttgat gttggttttg atgttaccca
    53161 agctcgattc acgccagatg accgagcttc tggatagcga acagcgtcag gggctgatga
    53221 ttgagcagca tgtcgaagcg gagctggcga acgatccgcc caacgattta atgtggtggc
    53281 ggcgtctgtt ccgggcgatt gataagtggg caccgccagg acagcgtttg ttattggtga
    53341 ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta
    53401 ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa gtatggccgc gtggaactgg
    53461 tcggtccgtt ctccgtgcgt gatggcgaag ataattacca actttatctg attcgtccgg
    53521 ccagcagttc tcaatccgat ttcattaact tactgtttga ccgcccgcta ttactgctga
    53581 ttgtcaccat gttggtcagt acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac
    53641 cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca gggaaactta cgccagcacc
    53701 cggaactgga agcggggcca caggaattcc ttgccgcagg tgccagtttt aaccagatgg
    53761 tcaccgcgct ggagcgcatg atgacctctc agcagcgtct gctttctgat atctctcacg
    53821 agctgcgcac cccgctgacg cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg
    53881 gtgaaagcaa ggaactcgag cgtattgaaa ccgaagcgca acgtctggac agcatgatca
    53941 acgatctgtt ggtgatgtca cgtaatcagc aaaaaaacgc gctggttagc gaaaccatca
    54001 aagccaacca gttgtggagt gaagtgctgg ataacgcggc gttcgaagcc gagcaaatgg
    54061 gcaagtcgtt gacagttaac ttcccgcctg ggccgtggcc gctgtacggc aatccaaacg
    54121 ccctggaaag tgcgctggaa aacattgttc gtaatgctct gcgttattcc catacgaaga
    54181 ttgaagtggg ctttgcggta gataaagacg gtatcaccat tacggtggac gacgatggtc
    54241 ctggcgttag cccggaagat cgcgaacaga ttttccgtcc gttctatcgg accgatgaag
    54301 cacgcgatcg tgaatctggc ggtacaggat tggggctggc gattgttgaa accgccattc
    54361 agcagcatcg tggctgggtg aaggcagaag acagcccgct gggcggttta cggctggtga
    54421 tttggttgcc gctgtataag cggagttaaa ctccgcattt gtaggcagga taaggcgttt
    54481 acgccgcatc cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc
    54541 ctacactccg ggggttattt tccccacaac cggcgggaaa aatcttcgat cttgccgctc
    54601 agtcggcgct tttgcatcgt ccgcgtccag cttttcgata acccggcggc ggagagtaaa
    54661 cggtgatact gatcgtcatc aaacggcata tgccatgcga tggcggcagc ctcctgcacg
    54721 gtcacatcgc tgacacggga aaccaactcc agcggcgcgt ccgcagacac tttccctagc
    54781 gctatcacgc tatacaacca ccccacctta cctgtgtttt gcatcaactg cgctatatcg
    54841 ctgatatcaa aatggtaatt gagtttatag caaggcgagc gcggctggct gacctgaatt
    54901 aatgcctctc cccagcggaa aatatcgccc atatgaacat tactttccgt gagaccgtcg
    54961 gttgagagat tttcgccaaa cgcaggcgca acaaacaact ccgcctgttc cggaaattcc
    55021 cgcacccagt agagatagtg ctcacgagga taatgacaca gcgctctgtc tggcccgccg
    55081 tgaacctttt tctccgcctg ctcgtcacct tccagcccca gctccgtcag catcaactca
    55141 ccatcaacct ggattttggc aattgcgctg ggtttgcctt cgggataagc ctgaatcttg
    55201 cctgtgtata catcaaccgg atatcgcatc tgttccctct ccacgagaaa tttccccatt
    55261 agaccatatt tattttatgt cagccgtgct gttttcgcgg taagtatcca ggcaggaact
    55321 caatagcaaa ccggacgaac gttagttcat ccggctgtgg ggattaagga agcaggttta
    55381 tttcaccgta cctaaaattt cgattttcgc tgctctggct ttgactttac gtgaccagat
    55441 agaggtaaga attggcacca gaatcgaagt cacaatgacc gccgtcgcta ccagcgaagt
    55501 tgctgccgga gccatcggtt taaacgcagg caccatttct gcaatcagca caggtgtcgc
    55561 taccgcggcc cctgcggaac tggaagcggc aattccggca gtgccatcgc caccgccaat
    55621 caatttatcg gcgataatca acggaatacc ggtcacgata attactgcca cacccaacag
    55681 gatccccagc aaaccagtct gggcaatcac agtcaaatca atggtattgc ccagcgcgaa
    55741 ggcaaagaat ggaatcagcg tttgcaccgc tttgctgaaa aattctcgta attcagggtc
    55801 aagattcccc agggcaaagc ccaccaggaa cggtaatact gcgccgacga aaacatgcgg
    55861 ttcaaacgag gcaatcccgg cagtgcccag aataatcatc gtcatgagcg gcccggactc
    55921 caacgacatc aacacaaatg ccccagcttc ttctttcgta ccgtactgct gcatgatgga
    55981 agcgtaaagt ccgccgttgg tcatatccat cgccgccacc agcgccagcg ttgaaagtcc
    56041 ggcaaagaat ccaacttcaa caccatgttc cggaataatg cgcgaggcaa ttgccgcaac
    56101 cacccacgcg acggcaattt tagttaccac cagcgtaccg gatttacgca gtaccgttcc
    56161 cgtcgcgctt aattttattg acgcccccat gcaaaaaaac cacaccgcca gaatgggcac
    56221 cgtaccggta atcatcccgt tggtgaatga tccaaaatat ttccccgccc ccggcgagaa
    56281 ggtgtggcac agtgcgccaa ggaatagcgg gacgagcatc atcccccccg ggattttctc
    56341 aatcgagcgt tttatctgca tttccatcac ctgtcggcat atttttaagg gctgatgcgt
    56401 aattcctcaa tggagcatca gtgttgatgg aaacgaagat agacattcat ttaatcaata
    56461 aaagtgatca aaaccacatc aattgaaacg ctgttttatt tttataggat cattgttttt
    56521 attttggaat tgatcacaaa aaaaccaccg ccgttggcga tggttcattt tttaagctga
    56581 tatgcggcct atacgcctca ttgcagcagg cggcaaatga ttattttttc gccgcaaaac
    56641 gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc tttgatgtag tccggacggc
    56701 ggttctggaa tttcaggtag taagcatgtt cccacacatc caggcccaga atcgggaagc
    56761 cggaagcgcc agaaatagct tcacccatca gcggagaatc ctggttagca gtagaaacca
    56821 ccgccagttt atcgcctttc agcaccagcc atgcccagcc ggaaccaaag cgggaagctg
    56881 ccgctttttc aaattctgct ttgaagttat caacggagcc gaagtcacgt tcgatagccg
    56941 ctttcaggtc accctgcagg gtggtgcctt ttttcagacc tttccagaac aggctgtggt
    57001 tagcgtgacc gccagcgttg ttgcgcagta cggttttctt gtctgctggc agctggtcca
    57061 gtttggtgat cagctcttca accggcaggt tggcaaattc tggcaggctt tccagcgccg
    57121 cgttggcgtt gtttacgtag gtctgatggt gtttggtgtg gtggatttcc atggtctgct
    57181 tatcgaagtg cggttccagg gcatcgtaag catacggcag ggatggcagg gtatagctca
    57241 tattcatctc cagtattgtc gggcggccga ttgttaatgc cgcgtaagca gttggttcat
    57301 tatagttaat taaatgatat tgaaaatgat tatcaatgcc gtacttttcg taagggtatg
    57361 gttttgcagg aaaatgcccg agatgtgaag caaatcaccc acttaatgcc gtgattgcca
    57421 gtaaatcgac aacggcggca acaggcgaaa ggttaatcga cagcacgatt tttacactca
    57481 tctcgtcgga gatgtgaagc gacgaaaaat gatgaggata agaagatgag taacgcgatt
    57541 acgatgggga tattttggca tttgatcggc gcggccagtg cagcctgttt ttacgctccg
    57601 ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt cagtcggtgg gattgtttcg
    57661 tggattattc tgccgtgggc catcagcgcc ctgttactac cggatttctg ggcgtattac
    57721 agctcgttta gtctctctac gctgctgcct gtttttctgt tcggcgctat gtggaggatc
    57781 ggtaatatca actatggcct gaccatgcgt tatctcggca tgtcgatggg aattggcatc
    57841 gccattggca ttacgttgat tgtcggtacg ctgatgacgc caattatcaa cggcaatttc
    57901 gatgtgctga ttaacaccga aggcggacgc atgacgttgc tcggcgttct ggtggcgctg
    57961 attggcgtag ggattgtaac tcgagccggg cagttgaaag agcgcaagat gggcattaaa
    58021 gccgaagagt tcaatctgaa aaaagggctg gtgctggcgg tgatgtgcgg cattttctct
    58081 gccgggatgt cctttgcgat gaacgccgca aaaccgatgc atgaagccgc tgccgcactt
    58141 ggcgtcgatc cactgtatgt cgctctgcca agctatgttg tcatcatggg cggcggcgcg
    58201 atcattaacc tcggtttctg ttttattcgt ctggcaaaag tgaaggattt gtcgctaaaa
    58261 gccgacttct cgctggcaaa accgctgatc attcacaatg tgttactctc ggcactgggc
    58321 gggttgatgt ggtatctgca attctttttc tatgcctagg gccacgcccg cattccggcg
    58381 cagtatgact acatcagttg gatgctgcat atgagtttct atgtattgtg cggcggtatc
    58441 gtcgggctgg tgctgaaaga gtggaacaat acaggacgcc gtccggtaac ggtgttgagc
    58501 ctcggttgtg tggtgattat tgtcgccgcc aacatcgtcg gcatcggcat ggcgaattaa
    58561 tctttctgcg aattgagatg acgccactgg ctgggcgtca tcccggtttc ccgggtaaac
    58621 accaccgaaa aatagttact atcttcaaag ccacattcgg tcgaaatatc actgattaac
    58681 aggcggctat gctggagaag atattgcgca tgacacactc tgacctgccg cagatattga
    58741 ttgatggtca ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc actgcacgat
    58801 gcctcatcac aaaatttatc cagcgcaaag ggacttttca ggctagccgc cagccgggta
    58861 atcagcttat ccagcaacgt ttcgctggat gttggtggca acgaatcact ggtgtaacga
    58921 tggcgattca gcaacatcac caactgcccg aacagcaact cagccatttc gttagcaaac
    58981 ggcacatgct gactactttc atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc
    59041 cccacgctac ctaagcgcca gtgtggttgc cctgcgctgg cgctaaatcc cggaatcgct
    59101 ccctgccagt caagattcag cttcagccgc tccgggcaat aaataatatt ctgcaaaacc
    59161 agatcgttaa cggaagcgta ggagtgttta tcgtcagcat gaatataaaa gagatcgcca
    59221 cgggtaatgc gataaggacg atcgttgagt acatgcagac cattaccgcg ccagacaatc
    59281 accagctcac aaaaatcatg tgtatgttcg gcaaagacat cttgcggata acggtcagcc
    59341 acagcgactg cctgctggtc gctggcaaaa aaatcatctt tgagaagttt taactgatgc
    59401 gccaccgtgg ctacctcggc cagagaacga agttgattat tcgcaatatg gcgtacaaat
    59461 acgttgagaa gattcgcgtt attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg
    59521 tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccaca
    59581 gcgataggcg atgtcagtga cgctggcctc gctgtggcgt agcagatgtc gggctttcat
    59641 cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc gtttgctgct taagctgccg
    59701 atgtagcgta cgcagtgaaa gagaaaattg atccgccacg gcatcccaat tcacctcatc
    59761 ggcaaaatgg tcctccagcc aggccagaag caagttgaga cgtgatgcgc tgttttccag
    59821 gttctcctgc aaactgcttt tacgcagcaa gagcagtaat tgcataaaca agatctcgcg
    59881 actggcggtc gagggtaaat cattttcccc ttcctgctgt tccatctgtg caaccagctg
    59941 tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga gacggatact gcccatccag
    60001 ctcttgtggc agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata
    60061 cagcacattg gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg
    60121 tacgaaacag accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc
    60181 cgtgccatgt tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc
    60241 ctgcgggagc cggagttcta tcgccacgga cgcgttacaa gacggaaaaa aatccacact
    60301 atgtaatacg gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag
    60361 gtcaggttct taccttaaat tttcgacgga aaaccacgta aaaaacgtcg atttttcaag
    60421 atacagcgtg aattttcagg aaatgcggtg cgcatcacat caccacaatt cagcaaattg
    60481 tgaacatcat cacgttcatc tttccctggt tgccaatggc ccattttcct gtcagtaacg
    60541 agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa tgaaattcag caggatcaca
    60601 ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc atccagtggg cgcgtgatgc
    60661 tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca
    60721 atgggctgca tagccaaaac ggttatgtca cctgggatgt ggatagcctg gaaagtgcca
    60781 ttcgccttgg attaaacaag gtgtgcgagg aagggattcg tatcgatagc attgggattg
    60841 atacctgggg cgtggacttt gtgctgctcg accaacaggg tcagcgtgtg ggcctgcccg
    60901 ttgcttatcg cgatagccgc accaatggcc tgatgccgca ggcacaacag caactcggca
    60961 aacgcgatat ttatcagcgt agcggcatcc agtttctacc cttcaatacg ctttatcagt
    61021 tgcgtgcgct gacggagcaa caacctgaac ttattccaca cattgctcac gctctgctga
    61081 tgccggatta cttcagttat cgcctgaccg gcaagatgaa ctgggaatat accaatgcca
    61141 ccaccacaca actggtcaat atcaatagcg acgactggga cgagtcgcta ctggcgtgga
    61201 gcggggccaa caaagcctgg tttggtcgcc cgacgcatcc gggtaatgtc attgggcact
    61261 ggatttgccc acagggtaat gagattccgg tggtcgccgt tgccagccat gataccgcca
    61321 gcgcggttat cgcctcgccg ttaaacggtt cacgcgccgc ttatctctct tctggcacct
    61381 ggtcattgat gggcttcgaa agccagacgc catttaccaa tgacacggcg ctggcagcca
    61441 acatcaccaa tgaaggcggg gcggaaggtc gctatcgggt gctgaaaaat attatgggct
    61501 tatggctgct tcagcgagtg cttcaggaac ggcaaatcaa cgatctcccg gcgcttatcg
    61561 ccgcgacaca ggcacttccg gcctgccgct tcatcatcaa tcccaatgac gatcgcttta
    61621 ttaatcctga cgagatgtgc agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccga
    61681 tcccgggaag tgatgctgaa ctggcgcgct gtattttcga cagtctggcg ctgctgtatg
    61741 ccgatgtgtt gcatgagctg gcgcagctgc gcggtgaaga tttctcgcaa ctgcatattg
    61801 tcggcggcgg ctgccagaac acgctgctca accagctatg cgccgatgcc tgcggtattc
    61861 gggtgatcgc cgggcctgtt gaagcctcaa cgctcggcaa tatcggcatc cagttaatga
    61921 cgctggatga actcaacaat gtggatgatt tccgtcaggt cgtcagcacc accgcgaatc
    61981 tgaccacctt tacccctaat cctgacagtg aaattgccca ctatgtggcg cagattcact
    62041 ctacacgaca gacaaaggag ctttgcgcat gaccactcaa ctggaacagg cctgggagct
    62101 ggcgaaacag cgtttcgcgg cggtggggat tgatgtcgag gaggcgctgc gccaacttga
    62161 tcgtttaccc gtttcaatgc actgctggca gggcgatgat gtttccggtt ttgaaaaccc
    62221 ggaaggttcg ctgaccgggg ggattcaggc cacaggcaat tatccgggca aagcgcgtaa
    62281 tgccagtgag ctacgtgccg atctggaaca ggctatgcgg ctgattccgg ggccgaaacg
    62341 gcttaattta catgccatct atctggaatc agatacgcca gtctcgcgcg accagatcaa
    62401 accagagcac ttcaaaaact gggttgaatg ggcgaaagcc aatcagctcg gtctggattt
    62461 taacccctcc tgcttttcgc atccgctaag cgccgatggc tttacgctct cgcatcccga
    62521 cgacagcatt cgccagttct ggattgatca ctgcaaagcc agccgtcgcg tttcggccta
    62581 ttttggcgag caactcggca caccatcggt gatgaacatc tggatcccgg atggtatgaa
    62641 agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg ctggcagcac tggatgaggt
    62701 gatcagcgag aagctaaacc ctgcgcacca tatcgacgcc gttgagagca aattgtttgg
    62761 cattggcgca gagagctaca cggttggctc caatgagttt tacatggggt atgccaccag
    62821 ccgccagact gcgctgtgcc tggacgccgg gcacttccac ccgactgaag tgatttccga
    62881 caagatttcc gccgccatgc tgtatgtgcc gcagttgctg ctgcacgtca gccgtccggt
    62941 tcgctgggac agcgatcacg tagtgctgct ggatgatgaa acccaggcaa ttgccagtga
    63001 gattgtgcgt cacgatctgt ttgaccgggt gcatatcggc cttgacttct tcgatgcctc
    63061 tatcaaccgc attgccgcgt gggtcattgg tacacgcaat atgaaaaaag ccctgctgcg
    63121 tgcgttgctg gaacctaccg ctgagctgcg caagctggaa gcggcgggcg attacactgc
    63181 gcgtctggca ctgctggaag agcagaaatc gttgccgtgg caggcggtct gggaaatgta
    63241 ttgccaacgt cacgatacgc cagcaggtag cgaatggctg gagagcgtgc gggcttatga
    63301 gaaagcgatt ttgagccagc gtgggtaaac actgccggat gcggcacgag cgccttatcc
    63361 ggcctacggg tcgaatgtcc atgcgggctg tcaccctcac cccggccctc tccctgaaag
    63421 ggcgagggag aaaaacatag cctcgtgcgg caacggagct gaacaccgct ccggacagtc
    63481 ccctcgcccc tacggggaga gggttagggt gaggggaaca agccgcacca tgtctaaaca
    63541 aggcaaccta atttacagga acacagaaca tgcaaaacat tacccagtcc tggtttgtcc
    63601 agggaatgat caaagccacc accgatgcct ggctgaaagg ctgggacgag cgcaacggcg
    63661 gcaacctgac gctgcgcctg gatgacgccg atatcgcgct ttatcacgac aacttccacc
    63721 cacaaccgcg ctatattccg ctcagccaac ccatgccttt actggcaaat acgccgttta
    63781 ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct cgatcctgca gctaacttag
    63841 gcgtcgtaaa agtcgacagc gatggcgcgg gctaccatat tctctggggg ttaaccaacg
    63901 aagccgttcc aacttctgaa cttccggctc actttctctc ccactgcgag cgcattaaag
    63961 ccaccaacgg taaagatcgg gtgatcatgc actgccacgc caccaacctg atcgccctca
    64021 cctatgtact ggaaaacgac accgcggtct ttactcgcca actgtgggaa ggcagcaccg
    64081 aatgtctggt agtattcccg gatggcgttg gcattctgcc gtggatggtg cccggcacgg
    64141 acgaaatcgg ccaggcgacc gcacaagaga tgcaaaaaca ttcgctggtg ttgtggccct
    64201 tccacggtgt gttcggttcc ggaccgacgc tggatgaagc ctttggttta atcgacaccg
    64261 cagaaaaatc agcacaagta ttagtgaagg tttattcgat gggcggcatg aaacagacca
    64321 tcagccgtga agagttgata gcgctcggca agcgcttcgc cgttacacca ctagccagtg
    64381 cgctggcgct gtaaggagca aacatgatcc gcaaagcctt tgtcatgcag gtaaaccccg
    64441 acgcccacga agagtatcag cgtcggcata atcccatctg gccggaactg gaagcagtgt
    64501 tgaaatctca cggtgtgcat aactacgcca tttacctcga caaagcgcgt aatctgctgt
    64561 ttgccatggt agagattgaa tctgaagaac ggtggaatgc ggtagccagt actgatgttt
    64621 gccaacgttg gtggaaatat atgaccgatg ttatgcccgc taacccagat aacagcccgg
    64681 tgagtagcga gctgcaagaa gtgttttacc tgccgtaatt gcccaatgtg ccggagatgc
    64741 ctccggcaca atattttaaa tttcgatttg aaaaattaaa catattacat ctcccggtaa
    64801 gttatatttc cctgatacat tgtgagtaaa tcacaaaaat aatgaataac ccattaatga
    64861 ttcatgtggt ttatttaaat aaccattatg tgcattactc cgcaaatctg acctttcact
    64921 ctgtctcaaa ttgtcattgt ttaccgacat atcgcctctc atc
//
LOCUS       DADLSB010000004        64362 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.004, whole genome shotgun sequence.
ACCESSION   DADLSB010000004 DADLSB010000000
VERSION     DADLSB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.004"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(37..2712)
                     /gene="adhE"
                     /locus_tag="JN331_000240"
                     /gene_synonym="adhC"
     CDS             complement(37..2712)
                     /gene="adhE"
                     /locus_tag="JN331_000240"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY7258482.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAEKSA"
     gene            3189..3836
                     /gene="ychE"
                     /locus_tag="JN331_000241"
     CDS             3189..3836
                     /gene="ychE"
                     /locus_tag="JN331_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY7258483.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            4574..6205
                     /gene="oppA"
                     /locus_tag="JN331_000242"
     CDS             4574..6205
                     /gene="oppA"
                     /locus_tag="JN331_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY7258484.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTAGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            6291..7211
                     /gene="oppB"
                     /locus_tag="JN331_000243"
     CDS             6291..7211
                     /gene="oppB"
                     /locus_tag="JN331_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY7258485.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            7226..8134
                     /gene="oppC"
                     /locus_tag="JN331_000244"
     CDS             7226..8134
                     /gene="oppC"
                     /locus_tag="JN331_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY7258486.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAISGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            8146..9159
                     /gene="oppD"
                     /locus_tag="JN331_000245"
     CDS             8146..9159
                     /gene="oppD"
                     /locus_tag="JN331_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY7258487.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            9156..10160
                     /gene="oppF"
                     /locus_tag="JN331_000246"
     CDS             9156..10160
                     /gene="oppF"
                     /locus_tag="JN331_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY7258488.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQEPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10213..10542)
                     /locus_tag="JN331_000247"
     CDS             complement(10213..10542)
                     /locus_tag="JN331_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY7258489.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10577..12037)
                     /gene="cls"
                     /locus_tag="JN331_000248"
     CDS             complement(10577..12037)
                     /gene="cls"
                     /locus_tag="JN331_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY7258490.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            12180..12353
                     /locus_tag="JN331_000249"
     CDS             12180..12353
                     /locus_tag="JN331_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY7258491.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(12411..13664)
                     /gene="kch"
                     /locus_tag="JN331_000250"
     CDS             complement(12411..13664)
                     /gene="kch"
                     /locus_tag="JN331_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY7258492.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(13964..14260)
                     /locus_tag="JN331_000251"
     CDS             complement(13964..14260)
                     /locus_tag="JN331_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY7258493.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            14475..15203
                     /gene="tonB"
                     /locus_tag="JN331_000252"
     CDS             14475..15203
                     /gene="tonB"
                     /locus_tag="JN331_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY7258494.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(15243..15641)
                     /gene="yciA"
                     /locus_tag="JN331_000253"
     CDS             complement(15243..15641)
                     /gene="yciA"
                     /locus_tag="JN331_000253"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY7258495.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(15746..16285)
                     /locus_tag="JN331_000254"
     CDS             complement(15746..16285)
                     /locus_tag="JN331_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY7258496.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(16315..17058)
                     /locus_tag="JN331_000255"
     CDS             complement(16315..17058)
                     /locus_tag="JN331_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY7258497.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(17086..17175)
                     /locus_tag="JN331_000256"
                     /pseudo
     CDS             complement(17086..17175)
                     /locus_tag="JN331_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            17415..18053
                     /gene="ompW"
                     /locus_tag="JN331_000257"
     CDS             17415..18053
                     /gene="ompW"
                     /locus_tag="JN331_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY7258498.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(18113..18619)
                     /locus_tag="JN331_000258"
     CDS             complement(18113..18619)
                     /locus_tag="JN331_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7258499.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(18665..19165)
                     /locus_tag="JN331_000259"
     CDS             complement(18665..19165)
                     /locus_tag="JN331_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7258500.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(19251..19430)
                     /locus_tag="JN331_000260"
     CDS             complement(19251..19430)
                     /locus_tag="JN331_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY7258501.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(19811..20617)
                     /gene="trpA"
                     /locus_tag="JN331_000261"
     CDS             complement(19811..20617)
                     /gene="trpA"
                     /locus_tag="JN331_000261"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY7258502.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGIFAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(20617..21810)
                     /gene="trpB"
                     /locus_tag="JN331_000262"
     CDS             complement(20617..21810)
                     /gene="trpB"
                     /locus_tag="JN331_000262"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY7258503.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPSGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(21822..23180)
                     /gene="trpCF"
                     /locus_tag="JN331_000263"
     CDS             complement(21822..23180)
                     /gene="trpCF"
                     /locus_tag="JN331_000263"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY7258504.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGL
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(23184..24779)
                     /gene="trpD"
                     /locus_tag="JN331_000264"
     CDS             complement(23184..24779)
                     /gene="trpD"
                     /locus_tag="JN331_000264"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY7258505.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAATLVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(24779..26341)
                     /gene="trpE"
                     /locus_tag="JN331_000265"
     CDS             complement(24779..26341)
                     /gene="trpE"
                     /locus_tag="JN331_000265"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY7258506.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            complement(26433..26477)
                     /gene="trpL"
                     /locus_tag="JN331_000266"
     CDS             complement(26433..26477)
                     /gene="trpL"
                     /locus_tag="JN331_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAY7258507.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            26615..27496
                     /gene="yciV"
                     /locus_tag="JN331_000267"
     CDS             26615..27496
                     /gene="yciV"
                     /locus_tag="JN331_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY7258508.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            27493..28113
                     /locus_tag="JN331_000268"
     CDS             27493..28113
                     /locus_tag="JN331_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY7258509.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            28214..29086
                     /gene="rluB"
                     /locus_tag="JN331_000269"
     CDS             28214..29086
                     /gene="rluB"
                     /locus_tag="JN331_000269"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY7258510.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(29115..29705)
                     /gene="cobO"
                     /locus_tag="JN331_000270"
     CDS             complement(29115..29705)
                     /gene="cobO"
                     /locus_tag="JN331_000270"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY7258511.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACRKVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(29702..30460)
                     /locus_tag="JN331_000271"
     CDS             complement(29702..30460)
                     /locus_tag="JN331_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY7258512.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            30680..31729
                     /gene="sohB"
                     /locus_tag="JN331_000272"
     CDS             30680..31729
                     /gene="sohB"
                     /locus_tag="JN331_000272"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY7258513.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(31765..32016)
                     /locus_tag="JN331_000273"
     CDS             complement(31765..32016)
                     /locus_tag="JN331_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY7258514.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            32396..34993
                     /gene="topA"
                     /locus_tag="JN331_000274"
     CDS             32396..34993
                     /gene="topA"
                     /locus_tag="JN331_000274"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY7258515.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            35203..36177
                     /gene="cysB"
                     /locus_tag="JN331_000275"
     CDS             35203..36177
                     /gene="cysB"
                     /locus_tag="JN331_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY7258516.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            36472..36636
                     /locus_tag="JN331_000276"
     CDS             36472..36636
                     /locus_tag="JN331_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY7258517.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            36639..36806
                     /locus_tag="JN331_000277"
     CDS             36639..36806
                     /locus_tag="JN331_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258518.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            36920..37015
                     /locus_tag="JN331_000278"
     CDS             36920..37015
                     /locus_tag="JN331_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258519.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            37179..39854
                     /gene="acnA"
                     /locus_tag="JN331_000279"
     CDS             37179..39854
                     /gene="acnA"
                     /locus_tag="JN331_000279"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY7258520.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYVLAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(39918..40508)
                     /gene="ribA"
                     /locus_tag="JN331_000280"
     CDS             complement(39918..40508)
                     /gene="ribA"
                     /locus_tag="JN331_000280"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY7258521.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHIALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            40678..41442
                     /gene="pgpB"
                     /locus_tag="JN331_000281"
     CDS             40678..41442
                     /gene="pgpB"
                     /locus_tag="JN331_000281"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY7258522.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKIHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41591..41899
                     /gene="lapA"
                     /locus_tag="JN331_000282"
     CDS             41591..41899
                     /gene="lapA"
                     /locus_tag="JN331_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY7258523.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            41906..43075
                     /gene="lapB"
                     /locus_tag="JN331_000283"
     CDS             41906..43075
                     /gene="lapB"
                     /locus_tag="JN331_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY7258524.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            43268..44005
                     /gene="pyrF"
                     /locus_tag="JN331_000284"
     CDS             43268..44005
                     /gene="pyrF"
                     /locus_tag="JN331_000284"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY7258525.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            44005..44331
                     /gene="yciH"
                     /locus_tag="JN331_000285"
     CDS             44005..44331
                     /gene="yciH"
                     /locus_tag="JN331_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY7258526.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(44457..44675)
                     /gene="osmB"
                     /locus_tag="JN331_000286"
     CDS             complement(44457..44675)
                     /gene="osmB"
                     /locus_tag="JN331_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY7258527.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(44944..45693)
                     /locus_tag="JN331_000287"
     CDS             complement(44944..45693)
                     /locus_tag="JN331_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7258528.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(45783..45956)
                     /locus_tag="JN331_000288"
     CDS             complement(45783..45956)
                     /locus_tag="JN331_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258529.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(46104..48089)
                     /gene="pdeR"
                     /locus_tag="JN331_000289"
                     /pseudo
     CDS             complement(46104..48089)
                     /gene="pdeR"
                     /locus_tag="JN331_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            48143..48245
                     /locus_tag="JN331_000290"
                     /pseudo
     CDS             48143..48245
                     /locus_tag="JN331_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(48323..50257)
                     /locus_tag="JN331_000291"
     CDS             complement(48323..50257)
                     /locus_tag="JN331_000291"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY7258530.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(50325..51452)
                     /locus_tag="JN331_000292"
     CDS             complement(50325..51452)
                     /locus_tag="JN331_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY7258531.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(51596..52384)
                     /gene="fabI"
                     /locus_tag="JN331_000293"
     CDS             complement(51596..52384)
                     /gene="fabI"
                     /locus_tag="JN331_000293"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY7258532.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(52752..53105)
                     /locus_tag="JN331_000294"
     CDS             complement(52752..53105)
                     /locus_tag="JN331_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY7258533.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(53173..53979)
                     /gene="sapF"
                     /locus_tag="JN331_000295"
     CDS             complement(53173..53979)
                     /gene="sapF"
                     /locus_tag="JN331_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY7258534.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(53981..54973)
                     /gene="sapD"
                     /locus_tag="JN331_000296"
     CDS             complement(53981..54973)
                     /gene="sapD"
                     /locus_tag="JN331_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY7258535.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(54973..55863)
                     /gene="sapC"
                     /locus_tag="JN331_000297"
     CDS             complement(54973..55863)
                     /gene="sapC"
                     /locus_tag="JN331_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY7258536.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(55850..56815)
                     /gene="sapB"
                     /locus_tag="JN331_000298"
     CDS             complement(55850..56815)
                     /gene="sapB"
                     /locus_tag="JN331_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY7258537.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(56812..58455)
                     /gene="sapA"
                     /locus_tag="JN331_000299"
     CDS             complement(56812..58455)
                     /gene="sapA"
                     /locus_tag="JN331_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY7258538.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(58768..59013)
                     /locus_tag="JN331_000300"
     CDS             complement(58768..59013)
                     /locus_tag="JN331_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY7258539.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDKIAEFLNQWGNE"
     gene            complement(59147..60532)
                     /gene="puuP"
                     /locus_tag="JN331_000301"
     CDS             complement(59147..60532)
                     /gene="puuP"
                     /locus_tag="JN331_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY7258540.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(60522..60686)
                     /locus_tag="JN331_000302"
     CDS             complement(60522..60686)
                     /locus_tag="JN331_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258541.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(60835..62253)
                     /locus_tag="JN331_000303"
     CDS             complement(60835..62253)
                     /locus_tag="JN331_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY7258542.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGYYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRRRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            62465..63229
                     /gene="puuD"
                     /locus_tag="JN331_000304"
     CDS             62465..63229
                     /gene="puuD"
                     /locus_tag="JN331_000304"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY7258543.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            63256..63813
                     /gene="puuR"
                     /locus_tag="JN331_000305"
     CDS             63256..63813
                     /gene="puuR"
                     /locus_tag="JN331_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY7258544.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            63963..>64362
                     /locus_tag="JN331_000306"
     CDS             63963..>64362
                     /locus_tag="JN331_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7258545.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAE"
BASE COUNT        16315 a        15812 c        16403 g        15832 t
ORIGIN      
        1 gaaggggccg tttatattgc cagacagcgc tactgattaa gcggattttt ccgctttttt
       61 ctcagcttta gccggagcgg cttctttttt cgctgcagtt tcaccttcta cataatcacg
      121 accgtagtag gtatccagca gaatctgttt cagctcggag atcagcgggt aacgcgggtt
      181 agcgccggtg cactggtcat cgaatgcatc ttcagacagt ttatccacgt tcgccaggaa
      241 gtctgcttcc tgaacgccag cttcacggat agatttcgga atacccagtt cagctttcag
      301 cgtttccagc catgccagca gtttctcgat cttagcagca gtacggtcgc ccggtgcgct
      361 cagacccagg tggtcggcaa tttcagcata acgacggcga gcctgcggac ggtcgtactg
      421 gctgaatgca gtctgcttgg tcgggttgtc gttcgcattg tagcgaataa cgttacaaat
      481 cagcagggcg tttgccagac cgtgcggaat atggaactgg gaacccagtt tgtgcgccat
      541 tgagtgacat acacccagga aggcgttcgc aaatgcgata cccgcgatag ttgctgcact
      601 gtgaacacgt tcacgcgcta ccggattttt agacccttcg tggtaggacg ctggcagata
      661 ttctttcagc agtttcagtg cctgcagagc ctgaccatca gagaactcag atgccagtac
      721 agaaacataa gcttccatgg cgtgagttac tgcgtccaga ccaccgaaag cacacaggga
      781 cttcggcatg tccataacca ggttggcgtc gacaatcgcc atatccgggg tcagcgcata
      841 gtccgccagc gggtatttct gaccagtagc gtcgtcagtt acaaccgcaa acggagtgac
      901 ttcagaacct gtaccagaag tggtggtgac agcgatcatt ttcgctttca cgcccatttt
      961 cgggaacttg tagatacgtt tacggatatc cataaagcgc agcgccagct cttcgaagtg
     1021 agtttccgga tgttcgtaca taacccacat gatcttcgcg gcgtccatcg gggaaccacc
     1081 acccagcgcg ataatcacgt ctggtttgaa ggagtttgcc agttctgcac ctttacgaac
     1141 gatgctcagg gtcgggtccg cttctacttc gaagaagact tcagtttcaa cgcctgctgc
     1201 tttcagtacg gaagtgatct gatcagcata accattgttg aacaggaagc ggtcagtcac
     1261 gatgagcgca cgtttgtggc catcagtaat cacttcatcc agcgcgattg gcagggagcc
     1321 acggcggaag tagatagatt tcggaagttt gtgccacaac atgttttcag ctcgcttagc
     1381 aacggttttc ttgttgatca ggtgtttcgg accaacgttt tcagagatgg agttaccacc
     1441 ccaggaacca caacccagag tcagggaagg tgcgagtttg aagttataca ggtcaccgat
     1501 accaccctga gaagccgggg tgttaatcag gatacgagct gttttcattt tctgaccgaa
     1561 gtaagaaacg cgagccggtt ggttatcctg gtcagtgtac aggcaagagg tatgaccgat
     1621 accgcccata gcaaccagtt tctctgcttt ttctaccgcg tcttcgaaat ctttagcgcg
     1681 gtacattgcc agagtcgggg acagtttttc atgtgcgaac ggttcgcttt catcaacaac
     1741 ggtcacttca ccgatcagaa tcttggtgtt ttctggtaca gagaagcctg ccagttcagc
     1801 aattttatag gctggctgac caacgatagc cgcgttcagc gcaccatttt tcaggataac
     1861 atcctgaaca gctttcagct ctttaccctg caacagatag ccgccgtggg ttgcgaaacg
     1921 ttcacgtact gcgtcataaa cagagtcaac aacaacaaca gactgttcag aagcacagat
     1981 tacgccgttg tcgaaggttt tggacatcag tacagatgca actgcacgtt tgatatcagc
     2041 agtttcatca ataacaactg gagtgttacc cgcgcctaca ccgatagctg gtttaccgga
     2101 gctgtatgcg gctttaacca tgcccggacc accagtcgcg aggatcaggt tgatgtctgg
     2161 gtggtgcatc agtgcgttag acagttcaac agaaggttga tcgatccagc cgatcagatc
     2221 tttcggagca ccggcagcga tagcagcctg cagaacgata tcagccgctt tgttggttgc
     2281 atcttttgca cgcgggtgcg gggagaagat aatggcgtta cgggtcttca ggctgatcag
     2341 cgatttgaag atagcagttg aagtcgggtt agtggtcgga acgataccgc aaataatacc
     2401 gattggttca gcgatagtga tggtaccaaa agtgtcgtct tcagacagaa caccacaggt
     2461 tttttcatct ttataggcgt tgtagatata ttcagaagca aagtggtttt tgatcacttt
     2521 atcttcgacg atacccatgc cggattcggc aacggccatt ttcgcgagtg ggattcgagc
     2581 atctgcagca gccagagcgg cggcgcggaa gattttgtct acttgctctt gagtgaaact
     2641 ggcatattca cgctgggctt tttttacacg ctctacgagt gcgttaagtt cagcgacatt
     2701 agtaacagcc ataatgctct cctgataatg ttaaactttt ttagtaaatc atctgctcga
     2761 atacgagagt atagtcagtg cggtgatgat ttgcttaacc gatgaaaatc aaaagcttac
     2821 tcgcgctcac actcactgtg atttactaaa agagtttaaa cattagagtt attatctcta
     2881 atgcgtcact tccaggtggc gtaagcaaga ttactcactt ctgggtactg attacgtgat
     2941 ccaaatcaaa tttttgcaaa gctgatacct ttcagcatcg cttttcgcca ttatagctaa
     3001 cagttaataa attgtagtat gatttggtgg ctacattagc atgttttgca caactagata
     3061 acaataacga atgatagcaa ttttaagtag ttaggaggtg aaaaatgctg tcaaaaggcg
     3121 tattgtcagc gcgtcttttc aaccttattt atggctaaca ttatccggct tttgcttcgg
     3181 agctaaccgt gattcagacc ctttttgatt ttcccgttta cttcaaattt ttcatcgggt
     3241 tatttgcgct ggtcaacccg gtagggatta ttcccgtctt tatcagcatg accagttatc
     3301 agacagcggc agcgcgaaac aaaactaacc ttacagccaa cctgtctgtg gccattatct
     3361 tgtggatctc gctttttctc ggcgacacga ttctacaact ttttggtata tcaattgatt
     3421 cgttccgtat cgccgggggc atcctggtgg tgacgatagc gatgtcgatg atcagcggca
     3481 agcttggcga ggataaacag aacaagcaag aaaaatcaga aaccgcggta cgtgaaagta
     3541 ttggtgtggt gccattggcg ttgccgttga tggcggggcc aggggcgatc agttctacca
     3601 tcgtctgggg tacgcgttat cacagcatta gctatctgtt tggtttcttt gtggctattg
     3661 cattgttcgc tttatgttgc tggggattgt tccgcatggc accgtggctg gtacgggttt
     3721 tacgccagac cggcatcaac gtgattacgc gtattatggg gctattgctg atggcattgg
     3781 ggattgaatt tatcgttact ggtattaagg ggattttccc cggcctgctt aattaattcc
     3841 tttcaaatga aacggagctg ccatgctccg tttacttcgt cattattttt actttgttcc
     3901 cgcgcagtta tcaaaagcaa aaggaatagg taaaaatatt cttctcaaat tacagttagt
     3961 tataaggatt tccttaactg cttctcctca ccatcatgtt attttcgcca catcataatc
     4021 ctgggcttgc tgaagaataa ttgaaatgat attagtaatt ccactgcctt tggtagagga
     4081 aagtgctaaa taataatcaa ttgttaaatt attgtgcatt tcactactgg aactgtaatc
     4141 agaaaagata gacatgctta gccaatctct atttgattga attgaaagat gtttgttaag
     4201 gcatggatgc aagctataga ttctgatacg gacaataaaa gagaatcgct taacaatttt
     4261 gcaaaatgta ttggcgagta agaaccgcat ttggtacttt ccgggcaacc gccagacgat
     4321 tctttattgg taatgagaat aattaacaat taaagagcgt cgcgaaagaa taatgtgtct
     4381 cgacagggga gacacagtac gaatcgacat aaggtgatcg tctgaatcac cagaataaat
     4441 aaagtcggtg atagtaatac gtaacgataa agtaacctga cagcagaaag tctccgagcc
     4501 tgtgcagggt cccaatccgg gattacacat gctggttaat accagtaatt ataatgaggg
     4561 agtccaaaaa acaatgacca acatcaccaa gagaagttta gtagcagctg gcgttctggc
     4621 tgcgctaatg gcagggaatg tcgcgctggc agctgatgta cccgcaggcg tcacactggc
     4681 ggaaaaacaa acactggtac gtaacaatgg ttcagaagtt cagtcattag atccgcacaa
     4741 aattgaaggt gttccggagt ctaatatcag ccgagacctg tttgaaggct tactggtcag
     4801 cgatcttgac ggtcatccag cacctggcgt cgctgaatcc tgggataata aagacgcgaa
     4861 agtctggacc ttccatttgc gtaaagatgc gaaatggtct gatggcacgc cagtcacagc
     4921 acaagacttt gtgtatagct ggcaacgttc tgttgatccg aacactgctt ctccgtatgc
     4981 cagttatctg caatatgggc atatcgccgg tattgatgaa attcttgaag ggaaaaaacc
     5041 gattaccgat ctcggcgtga aagctattga tgatcacaca ttagaagtca ccttaagtga
     5101 acccgttccg tacttctata aattacttgt tcacccatca acttcaccgg tgccaaaagc
     5161 cgctatcgag aaattcggcg aaaaatggac ccagcctggt aatatcgtca ccaacggtgc
     5221 ctatacctta aaagattggg tcgtaaacga acgaatcgtt cttgaacgca gcccgaccta
     5281 ctggaacaac gcgaaaaccg ttattaacca ggtaacctat ttgcctattg cttctgaagt
     5341 taccgatgtc aaccgctacc gtagtggtga aatcgacatg acgtataaca acatgccgat
     5401 cgaattgttc cagaagctga aaaaagagat cccggacgaa gttcacgttg atccatacct
     5461 gtgcacttac tattacgaaa ttaacaacca gaaaccgcca ttcaacgatg tgcgtgtgcg
     5521 taccgcactg aaactaggta tggaccgcga tatcattgtt aataaagtga aagcgcaggg
     5581 cgacatgccc gcctatggtt acactccacc gtatactgct ggcgcaaaat tgactcagcc
     5641 agaatggttt ggctggagcc aggaaaaacg taacgaagaa gcgaaaaaac tgctggctga
     5701 agcgggttat accgcagaca aaccgttgac catcaacctg cggtataaca cctccgatct
     5761 gcataaaaag ctggcgattg ctgcctcttc attgtggaag aaaaacattg gtgtaaacgt
     5821 caaactggtt aaccaggagt ggaaaacgtt cctcgacacc cgtcaccagg gcacttttga
     5881 tgtggcccgt gcaggctggt gtgcagacta caacgaacca acttccttcc tgaacaccat
     5941 gctgtcgaac agctcgatga ataccgcgca ttataagagc ccggcctttg acagcattat
     6001 ggcggaaacg ctgaaagtga ctgacgaggc gcagcgcaca gctctgtaca ctaaagcaga
     6061 acaacagctg gataaggatt cggccattgt tcctgtttat tactacgtga atgcgcgtct
     6121 ggtgaaaccg tgggttggtg gctataccgg caaagatccg ctggataata cctatacccg
     6181 gaatatgtac attgtgaagc actaatggca atacgtgggg caggagtgtc ctgctccacg
     6241 gtgtctgatt tttatcgcat tacagaaggc acaggccaga aggtagggca atgttaaaat
     6301 ttattctacg tcgctgtctg gaagcgattc cgacgctatt tattcttatt actatttcgt
     6361 tctttatgat gcgcctcgcg ccgggaagcc cttttaccgg cgaacgtact ttaccgccag
     6421 aagtgatggc caatatcgaa gcgaaatatc atcttaatga tccgatcatg acacagtatt
     6481 tcagctacct gaaacaactg gcgcatggcg atttcggtcc atcgtttaaa tataaagatt
     6541 attcggtcaa tgacctggtg gcatccagtt ttcccgtttc tgccaaactg ggagccgccg
     6601 catttttcct tgcggtaata ctgggtgtta gtgctggcgt tattgccgca ttaaaacaaa
     6661 acaccaaatg ggactatacc gtgatggggc tggcaatgac cggggttgtt atccccagtt
     6721 ttgtggttgc gccattatta gtcatgatat ttgcgatcat tttgcattgg ctgccgggcg
     6781 gtggctggaa tggtggggcg cttaaattca tgatattgcc gatggtggcg ttgtcactcg
     6841 cctatatcgc cagtattgcg cgtattaccc gtggctctat gattgaagta ttacactcca
     6901 actttattcg tactgcccgg gcgaaagggt tacctatgcg gcggatcatt ttacgccacg
     6961 cattaaaacc tgctctgtta cccgtgctct cctatatggg ccccgcattt gtcggcatta
     7021 ttaccggttc tatggtcatc gaaaccattt atggtttgcc ggggattggg caactgttcg
     7081 ttaatggcgc attgaaccgt gactattcct tagtgttaag cctgaccatc ctggttggtg
     7141 cgttaaccat tttgtttaat gccattgtcg atgtgctata tgcggttatc gacccgaaaa
     7201 tccgttactg atactggagc tcgcgatgat gttaagtaag aaaaacagcg agacgctgga
     7261 aaatttcagt gaaaagctgg aggtcgaagg gcgcagcttg tggcaggacg cacgtcgacg
     7321 ttttatgcat aaccgtgcgg cggttgccag tctgatagtg ctggtgctga tcgcgttatt
     7381 tgtaatcctg gcaccgatgc tttcccagtt tgcctatgac gatactgact gggcgatgat
     7441 gtccagcgcc ccggatatgg agtccggtca ctactttggt actgactcat ccggtcgcga
     7501 cctgcttgtg cgcgttgcga ttagcgggcg tatctcactc atggtcggtg ttgctgcggc
     7561 actggtggca gtggtcgtgg ggacacttta tggttcgctt tccggttatc tgggcggtaa
     7621 agtggattcg gtaatgatgc gtctgctgga aatcctcaac tccttcccat tcatgttctt
     7681 cgtcattttg ctggtgacct ttttcggcca aaacatcctg ctgattttcg tggcgattgg
     7741 catggtttcc tggctggata tggctcgtat tgtgcgtggg caaaccctga gtctgaagcg
     7801 caaagagttt attgaggcgg cacaagttgg cggtgtatcg acgccgggca ttgttattcg
     7861 ccacattgtg ccgaacgtac tcggtgtggt ggtggtctac gcatcgctac tggtgcccag
     7921 catgatcctc tttgaatctt tccttagctt cctggggttg ggtacgcaag agccgttaag
     7981 cagctggggg gcattgctga gtgatggcgc gaactcgatg gaagtctctc catggttact
     8041 gttgttccca gcgggattcc ttgtggtgac actgttttgt ttcaacttta tcggcgatgg
     8101 cttgcgtgat gccctcgacc cgaaagatcg ttaaggagtg cagccatgag cgtaattgaa
     8161 actgcaactg tgccgctcgc acaacaacag gctgacgcac tgctgaacgt gaaagatttg
     8221 cgtgtcacct ttagtacccc ggacggcgac gtcacggcgg tcaatgattt aaatttttcc
     8281 ctacgtgccg gagaaacgct gggcattgta ggtgagtctg gttcgggtaa atcgcaaact
     8341 gcatttgcgt tgatgggcct gttggctgcc aacggacgca ttggcggagc ggcaaccttc
     8401 aatgggcgtg aaatcctcaa tttgccagag cgtgaactca ataaactgcg cgctgaacaa
     8461 atctcaatga tttttcagga cccaatgact tcgttgaatc cctatatgcg cgtcggtgag
     8521 cagttgatgg aagtgctgat gctgcataag aacatgagca aagctgaggc gtttgaagag
     8581 tcggtgcgga tgctcgatgc ggtaaaaatg ccggaagcac gtaaacgcat gaaaatgtac
     8641 ccgcacgaat tttctggcgg catgcgtcag cgagtcatga ttgcgatggc attgctatgt
     8701 cgacctaagt tgctgattgc ggatgaaccc actacagcgc tggacgtcac cgtacaggcg
     8761 cagatcatga cgctattgaa tgaactgaag cgggaattta atactgccat cattatgatc
     8821 acccacgatc ttggcgtggt ggcggggatc tgtgacaaag tgctggtaat gtacgccggg
     8881 cgcacgatgg aatatggcaa tgcgcgcgat gtcttttatc aacccgttca tccttattct
     8941 atcggtttgc tcaacgcggt gccgcgtctt gatgcggaag gtgaaacaat gttgaccatc
     9001 ccgggtaatc cgccaaactt gctgcgatta ccgaaaggtt gcccgttcca gccacgttgt
     9061 ccacatgcga tggaaatttg tagtagcgca ccgccgctgg aagagtttac gcctggccgt
     9121 ctgcgtgctt gctttaaacc ggtggaggaa ctgttatgaa tgctgtaact gaaggaagaa
     9181 aagtcctcct tgaaatcgcc gatcttaaag tgcactttga aatcaaagat ggcaaacagt
     9241 ggttctggca accgccgaaa acgctcaaag ccgtcgatgg tgtcactctt cgcctgtatg
     9301 aaggggaaac attaggtgtg gtaggggaat cgggatgcgg taagtccacc tttgctcgcg
     9361 ccatcatcgg tttggtcaag gcgaccgacg gtcatgttgc ctggttaggt aaagagttgc
     9421 tgggcatgaa gcccgatgaa tggcgtgccg ttcgcagtga tattcagatg attttccagg
     9481 agccgttggc atcgctaaac ccgcgtatga ccatcggcga gatcatcgct gaaccactgc
     9541 gtacttatca tccgaaaatg tcacgccagg aagttcgcga gcgcgtgaag gcgatgatgc
     9601 tgaaagtcgg gttattgcct aacctgatta accgctatcc gcatgagttc tccggtgggc
     9661 agtgccagcg tatcgggatt gctcgtgctc ttattcttga accgaagctg attatctgcg
     9721 atgagccggt ttcggcgctg gacgtgtcaa ttcaggcgca ggtggtcaac ctgctccagc
     9781 agctgcaacg tgagatggga ttgtcattaa tttttattgc tcatgacctg gccgtggtaa
     9841 aacacatttc cgatcgtgtg ttggtgatgt atctcggcca tgcagtagaa ctggggacct
     9901 atgatgaggt ctaccacaat ccactacatc cttacaccag ggcattgatg tcggcagtcc
     9961 ccatacctga tccggatctg gagaagaaca aaaccatcca gttactggaa ggggaattac
    10021 cgtcgccgat caacccgcct tccggttgtg ttttccgtac ccgttgcccg attgccggtc
    10081 cggagtgcgc caaaacacgt cctgttctgg aggggagttt cagacacgcc gtttcttgcc
    10141 tgaaagtcga tccgctttaa aaacaataag ggctgacagt tgtcagccct ttttcacgct
    10201 aaaagcgatt atttattccc gccagatgat atggcaaagt ttgtgatctt tttcgcggca
    10261 taacagaatg cgggcgaaaa catcgttgat ttcaccatct tcactgtccg ccagaccaat
    10321 caccacttcg gcaaaaaagt ccgggttcaa atcgaaatca acatgttcct gccagtcttc
    10381 cgccggatcg aataactccg cgccgccgcg ctcttcaaac tgaagattga acagcagaac
    10441 atcagctgga tcgagattgt cggcagccag ttcgagaaaa atatcgtagg cctgctcaag
    10501 cgtttcatct tcagtcaggc gattgtttag atccatatcc atagtcacta cctgtttaac
    10561 ctctgttggc gacgttttac agcaacgggc tgaagaagta aaacagtcgc tcggcgacac
    10621 gttgccataa tggacgtttt agccataaac gggcatcgag cagacgtgaa cgcgaaatat
    10681 aatcgtcctg aacggcggca aggtctgcac caaaaccttt atcgtcgatt gccagggtaa
    10741 tctcgaaatt cagccacaga ctacgcatat caaggttaac ggtgccaacc agacttagtt
    10801 cgccatcgac cagcacgctc ttggtatgca gtaacccgcc ttcaaactga taaattttaa
    10861 ccccagcagc aagcagttcc gtaaagaatg cgcgactggc ccagccgacc agcatcgagt
    10921 cattttttcg cggaaggata atactgacat ccaccccgcg ctgcgccgcc gtgcaaatcg
    10981 catgaagtaa atcatcgctt ggcacaaagt agggcgtggt catgatcaaa tattcacgcg
    11041 ccgaataagc cgcagtcaat aatgcctggt gaatgagatc ttccggaaag ccggggccag
    11101 aagcaattgt gtgaatggtg tgaccgctgg cctgttcaaa cggcataata ttgacatctg
    11161 gtggtggcgg cagaatacgt tttccggttt caatctccca gtcgcaggaa taaataatcc
    11221 ccatcgcggt ggcgatgggg ccttccatac gcgccatcag atcaatccat tgccctacgc
    11281 ccgcatcttg tttgaagtag cgaggatcga ccatattcat gctgccggtg tacgcgatgt
    11341 aattatcgat catgatcatc ttgcgatgtt ggcgcaggtc catacggcgt aaaaacacac
    11401 gcatcagatt gacctttaag gcttcgacca cttcaatacc ggcattacgc attagctcgg
    11461 gccacgggct gcggaaaaaa gccacactcc cggcggagtc gagcatcaat cggcaatgaa
    11521 taccacgccg tgcagccgcc attaatgatt cagccacctg gtccgccatg ccgccgggct
    11581 gccagatata aaacaccatc tcaatattat ggcgcgcgag ctggatgtcg cggattaacg
    11641 cctgcatcac atcatctgac tcggtcatca gttgtagctg attccctttg accccagcga
    11701 tcccctgacg acgctcgcaa agcttgaata atggcgcagc gacactgcta ttttcttcgg
    11761 cgaagatatg cttacaggct ttaaggtcgt taagccattt tgcggtggaa ggccacatcg
    11821 ctctggcgcg ctcagcgcgg cgtttgccta aatggagctc gccaacggca agataggcaa
    11881 taattccgac taacggcaga atgtaaataa tcaacagcca ggccatcgcg gagggaactg
    11941 cgcgtcgttt cattagaatg cgtaaagtta cgcctgcaat gagcaaccag tatcccagaa
    12001 tggccaacca actcaccaac gtataaacgg ttgtcataga ttagaaatcc ttttgaaagc
    12061 gcattgttat gagtttacgc atatggattc atctggcaaa taaaaacgcg ggaaaagcac
    12121 tggtcagcgt cgggggcggg ggtataatga ccattctgtt attgcataga gtagttaaca
    12181 tgaagcggag tagaacggaa gtggggcgct ggcgcatgca gcgtcaggct agccgacgta
    12241 aatcgcgttg gcttgagggg caatcgcgcc gaaatatgcg tatccacagc atcaggaagt
    12301 gcattctaaa caaacagcgt aactcgttat tgtttgcgat ctacaatatc taaatgtact
    12361 gggcaccatt attgcggtgc ccaataagtt tactcttccg tctttaaact ctatttttgc
    12421 gccgattctt tactgtcagc tttcgtttct tgttcatcgt tatcgctaaa aataccatga
    12481 cctgagttca acaacattga aacgagcata tcattattaa tctcttcacc atttaaaact
    12541 cgcgccagaa tttcgctgcc aaacagttgc ggtgagagaa tgatatccgg atgtaccatc
    12601 ttaatcttat tcaggttttt gctatcactg acggcgagaa ctgttttgac atcactgctc
    12661 atatcttttg ccgagagtac aacaaacgcg ttatctgcat cgttatcact cagcgccaga
    12721 atggcccggc aacgatcgat tcccgctttc tttaatactg aactgtcatt actgtcaccg
    12781 gggataacat cagcgttatc gcctaagcgt tgctcaagtt gcttgatatc atcttcaggc
    12841 aagttgctga taaccgttac gttttgtccg cgttgattca gttgcaggat cgtattgatg
    12901 gcgagaatcg aatgtccgca aacaataaaa tgatctttac gatgcattgt atgattgttt
    12961 ccttttaaaa gtttgttgaa tcccccgcgg ataagcggac caaaaattga ggtcatagat
    13021 gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag tgaacaatcg tgctgattca
    13081 gaaacaggga caatatctcc atagccgacg gttgacatgg tttctatcga aaaatagaac
    13141 gcggtcatca aactttctat tcgcggatta aaaccttcgc ttaaataaag cgcaccgtag
    13201 gttgagtaaa acagtaatgt cgtgaaacta ataaatgcaa aaattgtccc ggctgcggcg
    13261 ctactgtggg agaagtcttt gcgcagtatc agcaaaaaca ccagcgtaaa aatgcaaaat
    13321 ccaatactaa atttcagcca gggataaaaa tgcagggtgt aaattagcgc tatcaacaac
    13381 aaaatgatac tgattgccca tgcgagcttt gcccggaaca gtaagccaat ggagttcagg
    13441 acaagaaaga cgccgagcat aaatagtggg gcgtttgcca gcaaggagag atccaactct
    13501 gaaaatgctt tgatatggaa aatatcaagc aaattgaccg acataccata gattgtttta
    13561 aaaataagca atccatttaa aaagacggcc agcgcgagaa tgtcgtgccg tagggtcacc
    13621 cataaatttg ttgctgtttg tttgaatgta gcccagtgac tcactgatac ctctgtaacc
    13681 acaattttgt ttaattatct tagcgtgcac attttctgaa catacatgca gcgcgtgaat
    13741 gtgttaaaca atagtagatg actttatccg cagtaatcaa aggttttgag tgagcaactc
    13801 tgcgtcagga aagggctttt ttcctgaaca tgctcacatc atataaagaa atacagataa
    13861 agttattatc tgcttgtggt agtgaatgca ctgaccggct ataaggaaag gccaaacaag
    13921 aacacggttg caaaaaccgt gcccttaaat attggatctc tactcagaac actttcttaa
    13981 atggtttcac tgaaacgtgt tcatagactc ctgccgctac gtacgggtca gcatcggccc
    14041 aggcctgagc tgcttccagc gattcaaatt cagcaataac ggttgagcca gtaaatcccg
    14101 cagcccctgg atcgttactg tctaccgctg gcattggacc agctgtcaac aaacgacctt
    14161 catcatgcag taactgtaaa cgtgctaaat gtgccggacg aacggaaagg cgtttttcga
    14221 ggctatcagc tttgtcttga gcgtaaataa catacaacac gggcacaact ccttgttcgg
    14281 gaaagttgta aagtacgtta tttgaaaggg cgaagatctg caacggaaag atgatgtctt
    14341 tgttaaggtc atgcataaag taagggtaat tacgccaaaa atgacatttt cactgatcct
    14401 gatcgtcttg ccttattgaa tatgattgct atttgcattt aaaatcgagg cttggttttt
    14461 ctactgaaat gattatgact tcaataaccc ttgatttacc tcgccgcttc ccctggccga
    14521 cgttactttc ggtctgcatt catggtgctg ttgtggcggg tctgctctat acctcggtac
    14581 atcaggttat tgaactacct gcgcctgcgc aaccaatttc tgtcacgatg gttgcgcctg
    14641 ctgatctcga accgccacaa gccgttcagc cgccaccgga gccggtggta gagccagaac
    14701 cggaacctga gccgatcccc gaaccgccaa aagaagcacc ggtggtcatt gaaaagccga
    14761 agccgaaacc taagccaaaa ccgaagccgg tgaaaaaggt acaggagcag ccaaaacgcg
    14821 atgtcaaacc cgtagagtcg cgtccggcat caccgtttga aaatacggca cccgcgcgcc
    14881 ccacatcaag tacggcaacg gctgcaacca gcaagccggt taccagtgtg gcttcaggac
    14941 cacgcgcatt aagccgtaat cagccgcagt atccggcacg agcacaggca ttgcgcattg
    15001 aagggcaggt taaagttaaa tttgacgtca cgccggatgg tcgcgtggat aacgtacaaa
    15061 tcctctcagc caagcctgcg aacatgtttg agcgtgaggt gaaaaatgcg atgcgcagat
    15121 ggcgttatga gccgggtaag ccaggcagtg ggattgtggt gaatatcctg tttaaaatta
    15181 acggcaccac cgaaattcag taagcagaaa gtcaaaagcc tccgaccgga ggcttttgac
    15241 tattactcaa caggtaaggc gcgaggtttt ccttcaggat caaccgcgac atacttaaat
    15301 aatgcttctg tcgctttata gcgttgcccg attggttcag acgctacttt tttcacccac
    15361 acttcaatat taatgctgac cgatgtcgtc cctttctgga cacagcgtgc atagcagcac
    15421 accacatcgc cgaccgcaac cggccgtaag aaagtcattc cttcaacccg cacagtcact
    15481 acgcgaccgt gggcaatttc ttttgccaga atagcgccgc caatatccat ttgtgacatt
    15541 aaccaaccac caaagatgtc accattggca ttggtatcgg cgggcatggc taaagtacgt
    15601 aaaacaagat cgccctgagg gacgttatgt gttgtagaca tggtaaaacc gacttaaaag
    15661 gaaaatcaca ggcgcgatgc tactatgatt ttcctgagga gaacagagga gatacagcgc
    15721 cagccccgaa ggactggcag tctggttagg atttatcttc ctgcggcatg tggcggtaga
    15781 tatagatacc gcttaacaat gtgaagatta aggtaagggc ggtcaggcca aagactttaa
    15841 agttgaccca aatattttgc ggcagccaga atgcgatgta gatgtttgcc agaccgcaaa
    15901 ggataaagaa aacagcccag gccagattta gcttcgacca taccggttgc ggcagcgtga
    15961 gttctttacc cagcatccgc tgaattagcg gctttttcat cacccattgg ctgactaaca
    16021 gggctcccgc aaacagagca taaatgactg taaccttcca tttaataaac tcatcattgt
    16081 ggaagaacag cgtcaagcca ccgaagacga ccaccagaac aaaagtgatc agggccatct
    16141 tctcaacctt acgaaagcga acccagctat agataagcac aatcgccgtg gcgacgatca
    16201 gcgctgcagt agccgcatag atgtcataaa tcttgtaaaa cgcgaaaaag acaaccagcg
    16261 gtaaaaaatc aagaaactgc ttcattttac gattccgtaa tcaaatgctt aaggttattg
    16321 gcgaatcaac atatacaggc ggaacagata gatgagcaat atggcagaaa tcaggttgct
    16381 caaggtgttc gccagtacgg caccaatttc cggggttaat gcggcaaaag aagaggcaaa
    16441 aagcagcaac agtgtttttg ccagcaacca gctcagtact gcgggtgcca ccagacgcat
    16501 attcgcccaa gtcagccgca tactgctacg catcgaggca aaaatgccca ttttgtcctg
    16561 taccagcatc accggtgcca gggccagtaa aatggccatg ataattcccg gaacgaccac
    16621 cagcatgatg ccaatctgta ctaaaagggt agtcagaaaa atcagaataa ataacttggg
    16681 caatatcggc gcactggcac caatagctcg cagcgcactg actctctgac ccgcagacac
    16741 cagctggata attaatatta cgcctccggc gagaatggcg ttaccgatta attctgaaaa
    16801 agtggacgcc gctgaagcct gcagcaaaat ttgttgctgt tccggtgaca tattctgaac
    16861 caggtcgaac aacccactac tgccgctaac gggcacgccg tcattgagct gcgcaagctg
    16921 tgcatcactg ggtgagaaaa catgccctaa caccactgtg ataaacgcac atagcaacga
    16981 taccaacaga atggtcataa attgattacg gaagaaattt ccggtgtcac ggtatacgga
    17041 ctgcgccgtg atagacatgc actctccttg agttttatgc aggtgttaat tagcgggcaa
    17101 ttgtaccctg gttaagcctc gggtggcagc atcaggtctt gtatggaaaa gcatatcttt
    17161 gtaaagcgga ggtaattgct ggccttcagg cgtgggcaaa ccgcggcaag tagcgatcga
    17221 gttaatattt gcgtagagaa aatatttaaa aattgattta aatcacatta accaggattc
    17281 tcaatgcaac ttctaaatta atccagatca ataaatggtg aattatcata tgtaatgtga
    17341 tctatgtagg atcatttgtt actccaatgt aggtatattc gtcacgtttt tataaccata
    17401 acgacggagc ggatatgaaa aagttaacag tggcggcttt ggcagtaaca actcttcttt
    17461 ctggcagtgc ctttgcgcat gaagcaggcg aattttttat gcgtgcaggt tctgcaaccg
    17521 tacgtccaac agaaggtgct ggtggtacgt taggaagtct gggtggattc agcgtgacca
    17581 ataacactca actgggcttg acgtttactt atatggcgac cgacaacatt ggtgtggaat
    17641 tactggcagc gacgccgttc cgccataaaa tcggcacccg ggcgaccggc gatattgcaa
    17701 ccgttcacca tctgccgcca acactgatgg cgcagtggta ttttggtgat gccagcagca
    17761 aattccgtcc ttacgttggg gccggtatta actacaccac cttctttgat aatggattta
    17821 acgatcatgg caaagaggcg gggctttccg atctcagtct gaaagattcc tggggagctg
    17881 ccgggcaggt gggggttgat tatctgatta accgtgactg gttggttaac atgtcagtgt
    17941 ggtacatgga tatcgatacc accgccaatt ataagctagg cggtgcacag caacacgata
    18001 gcgtacgcct cgatccgtgg gtgtttatgt tctcagcagg atatcgtttt taattccgca
    18061 caaaaacgac cccgtaatat acggggtcaa taaggacatg gtataaaggg gattatttct
    18121 tcgcttctac gccatcagtt tcagaacgaa ttaaaaattt ctcagttgtt tgcggaatat
    18181 gctgaatcag ccagtcggcc atttgctttt cctcatttaa aatcgcttcg atgatcggaa
    18241 ttgagactgt atcaccggca ttttttgctg ctgctaatag tgaggtgtaa caggcgattt
    18301 caaattgctc gaagacatat ccgctaatag agcctttaac tatttcatca gaagggaata
    18361 taccaccgat tgactgccca agcgcagcca ttttactcat ggaatcttta atgactgaac
    18421 gtgaaatgtc attacgatca agaatagttt ccagttgaac aatctggttt ttggtttcac
    18481 taagatgttg ttcaatacga gcgcgtagtt caggataatt atctatacgg ctggccatgg
    18541 attcaagcat agattcggct tgcttttcca ttgcgtgggc gtcacgtaac cagtcatgat
    18601 aatgttcaat acgattcatg aaaatgtcct ctttataaaa tgactaaaag ttaaaattca
    18661 tatttcaggc tttattttcg gctttcttat ttacgttatt aagggccaga tcagtcagtt
    18721 tgatgtcggt ggccttttct tcttccaggg tttctttcag aagcttcgct gctttacggt
    18781 aacctaattg ttcagccagc gtcgctaatg tcccgtaact ggcaatctca taatgctcga
    18841 ctttctgtgc tgcggcaatc agtgcggcat cacgcacttc gtttttctcg gtactttcga
    18901 tgacctcatt agcttcttca ataagacctt ccattgccac acatttcatg cgcttaattt
    18961 tcagatttga ttctgattcc acaacttgat caattcgttc aatctgtcca tgagtttcct
    19021 cgaggtgcgc atgaaaagcc tgacttaatt tttcatttga tgttgctctt gcgagttttg
    19081 ccagtgcccg ggttaattgt ttttctgcgc tgtaggtatc tgaaagcagt tgaataaata
    19141 catcttcaat ggtcttcata ttcatatttt tctccagtga aatcactgcg agccatacgg
    19201 cccgcagatg caattatctt tatcagctga tattaataat taacggtgaa tcaggatttg
    19261 cctgatttat taccaccgct ttgttgaccg ccttttttac ccgcttcaga tgcgcgttgc
    19321 ggatcatttt taaaattacc gccgctatgc tgaccgcctt tacggcctgc gtcggatgcc
    19381 ttctcacggt cttcggcgaa atttcctgaa ccaccacgat gttcggccat gttatttctc
    19441 ccgttgcgtt gcattgtttc attaatatga gtgttgtgtg tcgacactca ttaaaattag
    19501 tcgataatga gaattagtca aattaagcgc aacgagaaga tagaggaaaa atatattttg
    19561 aggaacattc tggatatatt aacaattacc tgaggaataa gtgacttaga gaaaattatt
    19621 gattttactg gtgttatgtt gcgggattaa tttgtttgat tatattactg ttgggcggaa
    19681 aaatgacgta agttgacgtt cgaccggggt aagcgaaacg gtaaaaagat aaatattaaa
    19741 tgaatttagg atttttccgg cttcattaaa gaaagttaaa atgccgccag cggaactggc
    19801 ggctgtggga ttaactgcgc gtcgccgctt tcatcggttg tacaaaaact ttcagtgccg
    19861 ccagcatttt ctctggctca ttaatatgtt gctcgatgat tttaacaatg gccgaaccag
    19921 aaatcgcgcc cgcagctcct gcatcaatcg ctgcttttac ctgatccggg gcgaaaatac
    19981 caaacccctg caatggaggt gcagcgttgt actctttcag cttcgcaacc agatgattga
    20041 ggggtaacgc ggcgcggttt tctgcgccgg tcacgcccgc tcgtgacagc aaatatgtgt
    20101 aaccacgacc gtaagaggct atctggcgca gcaggtcgtc atcggcattt ggcgggcaga
    20161 tgaagatagg tgcgacatta tggcgcaacg cggcctggcg gaagggcgcg gactcttcaa
    20221 ctggcacatc ggcaaccagc accgaatcga cgccgacttt ctcgcactgg gcataaaact
    20281 catcaatgcc tttgttaaac accagattgg catacatcaa caggccaatg ggaatggtcg
    20341 ggtgtttctg gcgaatcagt gccagcattt caaaacattg tgccggagtc acacctgccg
    20401 caaaggcgcg cagagtggcg ttttgaatgg tcgggccatc cgccagtggg tcggagaagg
    20461 ggatacctaa ctccagcgcg tcagcaccgg cttcaattag cgtatcgata attttcaatg
    20521 actgctcaat gcccggatca ccgagcgtga cgaaaggaac gaatgcgcct tctttgcgct
    20581 ccttcaactg ggcaaacaga gattcgtagc gttccatcag atttcccctc gtgctttcaa
    20641 aatatcgtga acggtgaaga tgtctttatc gccgcgaccg gaaaggttaa ccaccagtag
    20701 ctgctcttta tccgggtttt cgcgcatcat tttcagcgca tgggccaggg cgtgggagga
    20761 ttccagcgcc gggatgatcc cttcgtgcag gcacagcgtt ttgaaggctt caagggcttc
    20821 atcatcggta atagacacgt aatcagcgcg tccagtgctg ttaagatacg cgtgttgtgg
    20881 gccgacagac gggaaatcca gtccggcgga gatggagtaa gattcttcaa tctgcccgtc
    20941 ttcggtttgc atcatcggcg ctttcatacc gaaataaatg cccacgcgac catgttttag
    21001 cggtgcgccg tgctcgccag tttcgatacc gtgaccacta ggctccacac caatcaggcc
    21061 gacgttggtt tcattgatga aatcagcaaa catgccgatg gcattcgaac cgccgccaac
    21121 acaggcgata acggcatccg gcaggcgacc ttctctttcc agaatctgcg ctttggtttc
    21181 ttcgccaatc atccgctgaa actcacggac aatggtcgga taaggatgcg ggccagccgc
    21241 ggtgcccagc atatagtgcg cggtttcata actgccggac cagtcgcgta gcgcctcatt
    21301 acaggcatct ttcagcgtgg cggaaccgct atgtaccggg atcacttccg cacccattaa
    21361 gcgcatacga aaaacgttag gcgactggcg ttcaacgtct ttggcaccca tataaatacg
    21421 gcatttcagg ccgagcaggg cactggcaag ggccgacgcc acgccatgct gaccggcccc
    21481 ggtttcggcg atgatttcgg ttttacccat ccgcttcgcc agcaacgcct gccccagcac
    21541 ctggttggtt ttatgtgcgc cgccgtgcag caaatcttca cgcttgagat acagcgtggt
    21601 gttcgtcccg gctgtaatgt tctggcattt ggtcagtgcg gttggacgcc cggcatagtt
    21661 tttcagcagg tcgttgaact gggcttgaaa ttcaggatct ttttgcgcgc tgacaaaagc
    21721 ttcttccagc tggcgcagag caggcatcag gatttgtggc acgtacatgc cgccaaactc
    21781 accaaaatag gggttaagta atgttgtcat tgttcctttc cttaatatgc gcgcagcgtc
    21841 tggaaaaccg aggccaaaag acgtgcgtct ttgatgcccg gttgcgactc tacagcagaa
    21901 ttaaaatcaa gtccggcgca gccggtttgt gccgcttcca cgcagttatc tgcgcctaag
    21961 ccccccgcca gcagaacgtt gccaagcgat tgaccattta atagtgacca gtcaaaacgt
    22021 tgcccgcttc caccctggcc gttgtctaaa acatatttat caacgtgctg aaactcgcgg
    22081 gcgggcaggg tttcaccgac gcttaatgct ttccagatgg caacatgtgc tggcagagct
    22141 tcacgcagcg tatcgatata cagctgatct tcattaccat gcagttgcac tgccgccagc
    22201 gataacacct tagctttgtc caccacatcg gcaatatcgt gattgcggaa cacgccaaca
    22261 tactgcaacg gtgccgcagc catcacttcc tgcgcctgtt caacgttgac gcaacgcggt
    22321 gatgtcgcaa caaaaatcaa cccaccgtaa atcgcgcccg cgtcataagc tgctttagca
    22381 tcttgcccac gcgtcaggcc acatacttta ttctcaccca gcaacacccg gcgcacggcg
    22441 gcgtgcaaat cgtcatgggc catcaacgcc gaaccaatca gaaaaccgtt agcgaagtgg
    22501 cttaactcgc gcacctgagc gtaagtattg atgccggatt cgctgattac cgttacgttg
    22561 tgccccagtt tcggcgcaag ctcgcgggta cggttgagat caatcgacaa atcacgcaga
    22621 tcgcggttgt tgatgccaac gacctttgcc cctaatgcaa tggcgcgctc cagttcctct
    22681 tcattactga cttcggtcag cagccccatc tccagactgt gagcgacggc tgcaagctgg
    22741 cgatattggt cgtcatccag tactgaaagc attaataagc aggcatcggc ctggtaatag
    22801 cgcgccagat agatctggta agggtcgata atgaagtctt tacataaaat cggctgcggg
    22861 gcgatttggc tgacgatggg gaggaaatca aagctcccct gaaaatattt ctcatcagtc
    22921 agcaccgaaa ttgccgaagc gtaatgttta taaatggcgg caatgcgtgc cggatcgaaa
    22981 tcatcacgga tcacgccttt tgacggcgac gcttttttac actccagaat aaacgccgtg
    23041 cgtgcaccct gtagcgcatc ataaaaatgt cgtgtgctcg gctgaacctc attctgaaaa
    23101 ctggccagcg gttgctgctg tttgcgggtt tctacccaaa tcgccttgtc tgcgacgatt
    23161 ttcgctaaaa cggtttgcat catttaccct cgtgccgcca gcgcggtaac tctgtcgtaa
    23221 gcggaaccac tgcgcagtac ctcaagaacg gtttgcgcat tggcttgcag atcttcatgg
    23281 ccatgcaggc gcattaacat ggcgacgttc gcagcgacgg ctgcttcatg ggcggcgtcg
    23341 cctttacctt gtaacaagcg tgttaaaatg tcacggtttt cttcaggtgt tccgcctgcc
    23401 agttgctcct ggtggtaggg agtcaggcca aaatcttcag cggtcaattg atagctctta
    23461 attttgccgt catgcagctc ggcaacgatt gtcggcgcgt gtaatgaaac ttcatccatc
    23521 ccgccgctgt gcaccaccgc cgcgcgttga taccccagca cgcgcaaggt ttcggcaatc
    23581 ggcagcacca gttccggact ataaacacca attaacgcca gcggcggatg cgccgggtta
    23641 atcaatggcc ccagcacatt gaacagggtg cgggttttca gttgctggcg aaccggcatc
    23701 gcgtggcgga atccggtgtg atatttcggc gcaaagagga aacatacgcc taactcgtcc
    23761 agcgcctggc gcgatttatt ggcgttcata tcaagattaa taccgaatgc cgccagcaga
    23821 tccgacgagc cggatttact ggagacgctg cggttaccgt gtttcgccac tttcagccca
    23881 caggccgcgg cgacaaacgc actggcggta gaaatattga tactgttgct gccgtcaccg
    23941 ccagtaccga cgatatcagc aaacagataa tccgggcgcg ggaacggcgc tgcgttttcc
    24001 agtagcgcgg ttgctgcccc ggcgatctcg ttcgggtgct caccgcgaat tttcatgctc
    24061 accagcgtcg ccgccagttg ttccggcttc agctcgccac gcaccaccgc tgaaaacagc
    24121 tggtggcttt cttgttggct aagcgtctgc gcctgataca gtttttccag aatcggttgc
    24181 agcgtgttgg ctggctctag tttctgctgc gcccaggcca gcgtttgttc cagcaggcga
    24241 gcgccctggg tggtgagaat ggattccgga tggaactgga atccacaaac gcgatccgca
    24301 tcgtgacgta ctgccatcac catgccatta aaatgggcgt tgatggttaa accggccgga
    24361 atgttactgc caaccagcga gtgataacgc gccaccggca gcgggtttgt taatccggca
    24421 aacatcgcct gaccgtcatg ttcaatgctc gacgctttac cgtgaagaat ttcgcccgcc
    24481 tgaccgacat agcccccgta agcttcgaca attgcctgat gtccgaggca aatgccaata
    24541 atgggcagct tgccacgcaa gcgggtgagg agttccggca tacaaccggc ttcgctcggc
    24601 acaccggggc caggagaaag catcagtacc ggattgctca tcgtcgccag gcgttcaatt
    24661 aaggtctgcg ccggaatatg gttgcggtaa atcaccacgt tatgaccatt gctgcgcaac
    24721 tgatctgcca ggttgtacgt aaaagagtcg atattatcga gcagcagaat gtcagccatc
    24781 agaaagtctc ctgtgcatga tgcgcggtgg caatagcgcg cagtacagcg cgggctttgt
    24841 tacgggtttc gtcggcttcc gactgcggaa cagaatcaag gactacacca gcacccgctt
    24901 gcacggtggc gataccgttt tccaccagcg ccgagcggat cacaatgcag gtgtcgagat
    24961 cgccatgcgc ggtgaaataa cctaccgcgc cgccgtagct gccgcggcga cgaccttccg
    25021 cctcggcaat taactgcata gcgcgtactt tcggcgcacc gcttaacgtc cccatattca
    25081 tacaggcgcg ataagcgtgc agggcgtcaa gatcgtgacg cagttcgccg actacgcgag
    25141 agacgaggtg catcacatag gaataacggt caactttggt gagatcggcg acgtagcggc
    25201 tgccaggggt gcaaatgcgt gccagatcat tacgggcgag atcaaccagc atcagatgtt
    25261 cagacagctc tttatggtca gtacgcattt ccagttcaat acggctgtcg agatctctgt
    25321 ccagtgaacc atcggcgcga cgaccgcgtg ggcgtgttcc ggcaatcggg tagatctcaa
    25381 tctggcggct ggtggcatca tacttgagcg agctttccgg cgacgcgcca aatagggtga
    25441 aatcattatc ctgcataaaa aacatgtacg ggctgggatt actctttttc agcacgtaat
    25501 aggccgccag cggtgacggg cagggcagag agaaacggcg agatggcacc acctggaaaa
    25561 tttctccagc gcgaatcgct ttttgcaaca aacgcactac gccaccgaac tcttcatcgc
    25621 tctgattaca ttcacaacgc atatgcggca cggaaaccac tggcagcggc ggcgcggctt
    25681 cggtcagttg ctgacgtagt tcgttcaggc gagcagtgag acgttgtttt tcttcttcat
    25741 tcggagcaaa caggctggcc tgaatacggg tgcttttttt ctgatggtca atcaccatca
    25801 gcgtttcagc gagataaaaa cagaaatcag ggcagttatt ttccgctgac agttgcggta
    25861 aatcttcaaa tcccgccaca aggtcataag agaacaggcc gccgaagaac attgcttctc
    25921 gttcttcctt cggtacattc aacagattct gcaataaacg gaaagcgtca aaaaccgaaa
    25981 gggagcataa gcgggcgtct tcatccagca gtggactgac aggggggaag cgcagcacac
    26041 ggcagtttgg tgattgttca ctttccacac ccgcaggcag ggcgttatcc agtagtgcca
    26101 gcagggcttc gccattgccg gaaagtgcct gaattgtgac ggtgtcacct aatgctgtaa
    26161 tgcgcagcgc actgtctatc agcagcaggc tttttaaatc atctttgctg tcgatatctg
    26221 cggattccag cagcagcgtt gccggacgat tcccacacaa ctggtgaaaa agcgcagtcg
    26281 ggttgtcgcg ataagcgcct tcgcaggtta gcagttcgag agtcggtttt tgtgtttgca
    26341 ttgttattct ctaattttgt tcaaaaaaaa gcccgctcat taggcgggct gggtatctga
    26401 ttgctttacg catggtgaat acactgcccg tttcaggaag tgcgccacca acctttcagt
    26461 acgaaaattg ctttcattgt cgataccctt tttacgtgaa ctagcgtact agttaactgg
    26521 ttcgatgatt aattgtcaac agctcatttc agaatatttg ccagaaccgt tatgatgtcg
    26581 gcgcaaaaaa cattatccag aacgggagtg cgccttgagc gacacgaatt atgcagtgat
    26641 ttacgacctg cacagccata ccacagcttc cgatggctgc ctgacgccag aagcattggt
    26701 gcaccgtgca gtcgagatgc gcgtcggcac cctggcgatc accgaccatg acaccacagc
    26761 tgcaatcgcg ccagcaagag aggaaatttc acgttcggga ctggcgctga atcttattcc
    26821 cggcgtggaa atttccacgg tctgggaaaa tcatgaaatt catattgtcg ggctgaatat
    26881 tgatattact catccgctaa tgtgtgagtt cctcgcgcag cagacagaac ggcgcaatca
    26941 gcgggcgcag caaattgccg aaagacttga aaaagcgcaa atacctgggg cgctggaagg
    27001 cgcacaacga ctggcgcagg gcggcgcagt gacgcgcggt cattttgcgc gtttccttgt
    27061 ggagtgcggc aaagccagtt caatggcaga tgtctttaaa aagtatctgg cgcgcgggaa
    27121 aaccggatac gttccgccac agtggtgtac aatagaacaa gctattgatg tcattcatca
    27181 ttctggcggt aaggcggtgc tagctcatcc tgggcggtac aatcttagtg ctaaatggct
    27241 gaaaagattg gttgcgcatt ttgccgaaca ccacggcgac gcgatggaag tcgcgcagtg
    27301 ccagcaatcg cccaatgaac gcacccagct ggccacactt gcgcgtcagc atcatttatg
    27361 ggcatcacaa ggatctgatt ttcatcagcc atgcccgtgg atcgaactgg gtcgtaaact
    27421 ctggctgccc gcaggcgttg aaggcgtctg gcagctatgg gaacagccgc agaacaccac
    27481 agagagggaa ttatgagcca gtttttttat attcatcctg ataacccgca gcaacgtctg
    27541 atcaaccagg cggtggagat cgtgcgtaaa ggcggggtga ttgtctatcc aactgattcc
    27601 ggctatgcgc tcggctgtaa aattgaagat aaaaacgcca tggagcgtat ttgtcgtatt
    27661 cgccagctgc cggacggtca caactttacc ctgatgtgtc gcgatctttc tgaactctcg
    27721 acctattcat tcgtggataa tgttgcgttt cgtttgatga aaaacaacac gccgggcaac
    27781 tataccttca tcctgaaggg gacgaaggaa gtgccacgcc gtctgttgca ggagaaacgc
    27841 aaaaccatcg gtatgcgtgt gccttctaac ccgattgcgc aggcgttgct tgaagcactg
    27901 ggcgaaccga tgctttccac ttcgctaatg ctgccaggca gcgaatttac cgaatccgat
    27961 cccgaagaaa ttaaagaccg tctggaaaaa caggtagatt tgattattca cggcggctat
    28021 ctcgggcaga aaccaacaac ggttattgat cttaccgatg atacgccggt cgtggtgcgt
    28081 gaaggcgtag gtgatgtgaa gcctttctta taagtgcgta actctgtata ctacgcggcc
    28141 ttaaaatggc gctatacggc gccattgatt ctcctgttcg acgcctgtga aggcgacacc
    28201 aaaaggaagc tctatgagcg aaaaattaca gaaagtgctg gcccgcgctg gccacggctc
    28261 ccgccgtgaa atcgaatcta ttattgaagc cggtcgtgtg agtgttgatg gcaaaattgc
    28321 taaactcggc gatcgcgttg aagttacccc tgggctgaaa attcgtatcg atggtcatct
    28381 gatttcggta cgtgagtccg ctgaacaaat ttgtcgcgtt ctggcctatt acaaaccgga
    28441 aggtgagttg tgtacccgta acgatccgga agggcgtccg acggtgtttg accgtctgcc
    28501 aaagctgcgt ggcgcacgct ggattgccgt aggtcgtctg gacgttaaca cctgtggtct
    28561 gctgctgttc accaccgatg gtgaactggc aaaccgttta atgcatccaa gccgtgaagt
    28621 tgaacgtgaa tacgccgtgc gtgtatttgg tcaggttgac gacgcgaaac tgcgtgattt
    28681 gagtcgtggc gtgcaactgg aagatggtcc ggcagctttt aaaaccatca agttcagcgg
    28741 cggcgaaggg atcaaccagt ggtacaacgt gactctgacc gaaggccgta accgtgaagt
    28801 tcgtcgtctg tgggaagcgg ttggtgtgca ggtgagccgc ctgatccgtg ttcgttacgg
    28861 tgatatccca ctgccaaaag gtctgccacg cggtggctgg acggagctgg acctcgccca
    28921 gactaactat ctgcgcgaac tggtggagct tccgccagaa accagctcta aagtcgctgt
    28981 agaaaaagac cgtcgtcgca tgaaggctaa tcagattcgt cgtgcggtga aacgtcacag
    29041 tcaggtgagc ggcggtcgtc gttctggcgg gcgtaataac ggttaatcaa agtatgccgg
    29101 acgtcatatc cggattaata atcaatccct atttgcgcct taacaccggc atcaaacgca
    29161 tgtctgatgg ggcgcagctc acttaccgta tccgccagtt caagaatatc ccgatgacag
    29221 cctcgacccg tgataatcac cgtctgttga tgtggacgtt catttaacgc ctgcaccact
    29281 tcttccagtg gcaaatagtc atacgccacc atatacgtca gttcatcaag caaaaccata
    29341 tccagtgagg aatcagcaag catccgcttt gcatgttgcc agactttgcg gcaggcggcg
    29401 gtatcagact cgcggttttg tgtatcccag gtaaagcccg ttgccatcac ctgaaactca
    29461 acgccatgtg gctccagcag attgcgttcg ccattaggcc aggtgccttt aataaactgc
    29521 acgacgccca ctttttttcc gtgaccaact gcgcgtgttg ccgtaccaaa tgccgcggtg
    29581 gttttgcctt ttccattgcc ggtaaagacg atgataatac cgcgttcatc ctgggcctgg
    29641 gccacacggg catctacttt ttctttcact cgctgctgac gctgttggta gcgttcatca
    29701 ctcattggga aattcctggt ttacggcccg gttgggcgtc aaaggtcatg ccggttttac
    29761 ggcggctgtc atcgcccatc agccagaggt agagcggcat gatatcagcg ggtgttttaa
    29821 gtttctgtgg atcttcggtc gggaaggcgc ttgcgcgcat tgcggtgcgc gtaccgcctg
    29881 ggttaatgca gttgacgcgc aggcgctgct gatattcatc ggccagtacc tgcatcatcc
    29941 cttcggtggc aaatttcgac gctgcatatg caccccagtt ggctcgtccc tgacgtccaa
    30001 cgcttgatga agtaaagacc agtgaaccgg cgtccgattt gagtaataaa ggaagcagtg
    30061 cctgggtgag cataaaggtg gcattaacgt tgacctgcat gacgtcctgc cagacctgcg
    30121 gattttgttc gctcattggg caaacatcgc cgagcaatcc ggcattatgc aaaacaccat
    30181 ccagacgcgg ataattaacg acaatgcgct gtgccagttg ttggcaattt tcggacgtgc
    30241 aggtcagcaa atcgagaata aaccactgtg gctgacgccc agtttcttcg tttatgtggc
    30301 tggctacctg acgtaatttt tcttcattac ggcccaacag aatcactgtc gcaccatagc
    30361 gtgcatacgt catcgcggct tcacgaccaa tcccatcgct ggctcccgtc accaggataa
    30421 tgcgatcatt gagtaaatct tgttttggct ggtaatgcat ggctactcct caacgacgtt
    30481 gtctgtcgcg ctgccagtgt actttatgac tttggggctt tatgcctgaa acagaagggc
    30541 atttcaatca gcctggggcg ataacaccgc aaaatggata ctttgtcata ctttcgctgc
    30601 aataacatct ctgcgagacg gcttaacatg cctgttgtaa actgtgagcc aaagcgttgt
    30661 ttaaccaagg tggggactcg tggaattgtt gtctgaatat ggtttgtttt tggcgaaaat
    30721 cgttaccgtt gtgctagcga ttgcggcgat tgccgccatt attgtcaatg ttgctcaacg
    30781 taataaacgc cagcgtggcg agttacgggt caacaatctc agcgaacagt ataaggagat
    30841 gaaagaagaa ctggccgcgg cgctgatgga ctcacatcag caaaaacagt ggcacaaagc
    30901 gcagaagaaa aagcacaagc aagaagcgaa agcagcaaaa gcgaaagcca aactgggcga
    30961 ggtggcaact gacagtaaac ctcgcgtctg ggtgctggat tttaaaggca gcatggacgc
    31021 ccatgaagtg aactcgctac gtgaagagat aacggcggta ctcgcagcat tcaaaccgca
    31081 ggatcaggtt gtgctccgtc tggaaagccc tggtggcatg gtgcatggtt acgggttggc
    31141 ggcttcgcag ctgcagcgtc tgcgtgataa aaacattcct ttaactgtta cggtagacaa
    31201 agtcgctgcc agcggcggtt acatgatggc ctgtgtggcg gacaaaattg tttccgcacc
    31261 gtttgctatt gtgggttcca ttggggtggt ggcgcaaatg cccaacttta accgcttcct
    31321 gaaaagcaaa gatattgata tcgaactgca caccgccggg cagtataagc gcacgctgac
    31381 cttgctgggt gaaaataccg aagaagggcg ggagaaattc cgcgaagagc tgaacgaaac
    31441 gcatcagtta tttaaagatt ttgtgaagcg tatgcgtccg tctctggata ttgaacaggt
    31501 ggcaacgggt gaacactggt acggacaaca ggcggtagag aaaggcctgg ttgatgaaat
    31561 caacaccagt gatgaagtta ttcttagcct gatggaaggc cgtgaagtgg ttaatgtacg
    31621 ctatatgcag cgtaaacgac tcattgaccg attcaccggc agcgcggcag agagcgccga
    31681 tcgattgttg ctacgctggt ggcagcgggg tcaaaagcca ttgatgtaaa agacaaacgc
    31741 gaggctaaga cctcgcgttt tgctttaatc aaccagatga tatttttctg aaagcacatg
    31801 ggccaggtgt ttgaacatat taaacaccgc ggtgcttttg gctgttggca atccttgttc
    31861 atctaaaaag tagtcgccgg taaataccag cacgccatta cgctgcgtga cgccggtggc
    31921 ttcaatccct gcgagcgtat cctcatgctc acgaatgatt ttgttggcct ctttcaacag
    31981 cgtttcgcgg tcgatgggtt gtgtctcttt gttcattatt tactccttaa acaaggacat
    32041 tagtctacgc caggcatggc ttgcagacaa atataccacg ctggtggcaa gagcgcctta
    32101 ctggcaactt tggattttgc atgctaataa agttgcgtat cggattttat caggtacagt
    32161 gtgacgcttt cgtcaatctg gcaatagatt tgcttgacat tcgaccaaaa ttccgtcgtg
    32221 ctatagcgcc tgtaggccaa gacctgttaa ctcagtcacc tgaattttcg tgaacagagt
    32281 cacgacaagg ggttgatatc cgcagagagc gagtccatat cggtaactcg ttgccagtgg
    32341 aaggtttatc aacgtgcgac gcattcctgg aagaatcaaa ttaggtaagg tgaatatggg
    32401 taaagctctt gtcatcgttg agtccccggc aaaagccaaa acgatcaaca agtatctggg
    32461 tagtgactac gtggtgaaat ccagcgtcgg tcacatccgc gatttgccga ccagtggctc
    32521 agctgccaaa aagagtgccg actctacctc caccaagacg gctaaaaagc ctaaaaagga
    32581 tgaacgtggc gctctcgtca accgtatggg ggttgacccg tggcacaatt gggaggcgca
    32641 ctatgaagtg ttgcctggta aagagaaggt cgtctctgaa ctgaaacaac tggctgaaaa
    32701 agccgaccac atctatctcg caaccgacct tgaccgcgaa ggggaagcca ttgcatggca
    32761 cctgcgggaa gtgattggtg gtgatgatgc gcgctatagc cgagtggtgt ttaacgaaat
    32821 tactaaaaac gcgatccgcc aggcatttaa caaaccgggt gagctgaata ttgatcgtgt
    32881 taatgcccag caggcgcgtc gctttatgga ccgcgtggtg gggtatatgg tttcgccgct
    32941 gctgtggaaa aagatcgctc gtggtctgtc tgccggtcgt gtgcagtcgg tggcggtccg
    33001 cctggtggta gagcgtgagc gtgaaattaa agcgttcgta ccggaagagt tctgggaagt
    33061 cgatgccagc acgaccacgc catctggtga agcgttggcg ttgcaggtga ctcatcagaa
    33121 tgacaaaccg ttccgtccgg tcaacaaaga acaaactcag gctgcggtaa gtctgctgga
    33181 aaaagcgcgc tacagcgtgc tggaacgtga agacaaaccg acaaccagta aacctggcgc
    33241 tccttttatt acctctacgc tgcaacaagc tgccagcacc cgtcttggtt ttggcgtgaa
    33301 aaaaaccatg atgatggcgc agcgtttgta tgaagcaggc tatatcactt acatgcgtac
    33361 cgactccact aacctgagtc aggacgcggt aaatatggtt cgcggttata tcagcgataa
    33421 ttttggtaag aaatatctgc cggaaagtcc gaatcagtac gccagcaaag aaaactcaca
    33481 ggaagcgcac gaagcgattc gtccttctga cgtcaatgtg atggcggaat cgctgaagga
    33541 tatggaagca gatgcgcaga aactgtacca gttaatctgg cgtcagttcg ttgcctgcca
    33601 gatgacccca gcgaaatatg actccacgac gctgaccgtt ggtgcgggcg atttccgcct
    33661 gaaagcacgc ggtcgtattt tgcgctttga tggctggaca aaagtgatgc ctgcactgcg
    33721 taaaggcgat gaagatcgta tcttaccggc agtcgataaa ggcgatgctc tgacgctcgt
    33781 tgaactaaca ccagcccagc actttaccaa gccgccagcc cgtttcagtg aagcatcgct
    33841 ggttaaagaa ctggaaaaac gtggtatcgg tcgtccgtct acctatgcgt cgatcatttc
    33901 gaccattcag gatcgtggct atgtgcgagt agaaaatcgt cgtttctatg cggaaaaaat
    33961 gggcgaaatc gtcaccgatc gccttgaaga aaatttccgc gagttaatga actacgactt
    34021 taccgcgcag atggaaaaca gcctcgacca ggtggcaaat cacgaagcag agtggaaagc
    34081 tgtactggat cacttcttct cggatttcac ccagcagtta gataaagctg aaaaagatcc
    34141 ggaagagggt ggtatgcgcc cgaaccagat ggttctgacc agcattgact gcccgacttg
    34201 tggtcgcaaa atggggattc gcacagcgag caccggggta ttccttggct gttctggcta
    34261 tgcgctgccg ccgaaagagc gttgcaaaac caccattaac ctggtgccgg aaaacgaagt
    34321 gctgaacgtg ctggaaggcg aagatgctga aaccaacgcg ctgcgcgcaa aacgtcgttg
    34381 cccgaaatgc ggcacggcga tggacagcta tctcatcgat ccgaaacgta agttgcatgt
    34441 ctgtggtaat aacccaacct gcgacggtta cgagatcgaa gagggcgaat tccgcattaa
    34501 aggttatgac ggcccgatcg ttgagtgtga aaaatgtggc tctgaaatgc acctgaaaat
    34561 ggggcgtttc ggtaaataca tggcctgcac caacgaagag tgtaaaaaca cacgtaagat
    34621 tttacgtaac ggcgaagtgg caccaccgaa agaagatccg gtgccattac ctgagctgcc
    34681 gtgcgaaaaa tcagatgctt atttcgtgct gcgtgacggt gctgccggtg tgttcctggc
    34741 tgccaacact ttcccgaaat cgcgtgaaac gcgtgcgcca ctggtggaag agctttatcg
    34801 cttccgcgac cgtctgccgg aaaaactgcg ttatctggcc gatgcgccac agcaggatcc
    34861 ggaaggtaat aagaccatgg ttcgctttag ccgtaaaacc aaacagcaat atgtctcttc
    34921 ggaaaaagac ggaaaggcga ctggctggtc agcattttat gttgatggca aatgggttga
    34981 aggaaaaaaa taacctttaa ttctgtcagg tttttataaa caaagggtcg cgaaagcggc
    35041 ccttttttat tgcatattat tttttcttca cacctataca ctaaggctat aaatgatata
    35101 gtggttatag ttagcacctt ttttattatt aaatcgtatt agtcacccgc caggtgtgac
    35161 gaaaaaacga tgttctgatg gcgtctaagt ggatggttta acatgaaatt acaacaactt
    35221 cgctatattg ttgaggtggt caatcataac ctgaatgtct catcaacagc ggaaggactt
    35281 tacacatcac aacccgggat cagtaaacaa gtcagaatgc tggaagacga gctaggcatt
    35341 caaatttttt cccgaagcgg caagcacctg acgcaggtaa cgccagcagg acaagaaata
    35401 attcgtatcg ctcgcgaagt cctgtcgaaa gtcgatgcca taaaatcggt tgccggtgag
    35461 cacacctggc cggataaagg ctcgctgtat atcgccacca cgcataccca ggcacgctac
    35521 gcgttaccaa acgttatcaa aggctttatt gagcgttatc cccgcgtttc tttgcatatg
    35581 caccagggct cgccgacaca aattgctgat gccgtctcta aaggcaatgc cgatttcgct
    35641 atcgccacag aagcgctgca tctgtatgaa gatttagtga tgttaccgtg ctaccactgg
    35701 aatcgggcta ttgtagtcac tccggatcac ccgctggcag gcaaaaaagc cattaccatt
    35761 gaagaactgg cgcaatatcc gttggtgaca tataccttcg gctttaccgg acgttcagaa
    35821 ctggatactg cctttaaccg cgcagggtta acgccgcgta tcgttttcac ggcaacggat
    35881 gctgacgtca ttaaaactta cgtccggtta gggctggggg taggggtcat tgccagcatg
    35941 gcggtggatc cggtcgccga tcccgacctt gtgcgcgttg atgctcacga tatcttcagc
    36001 cacagtacaa ccaaaattgg ttttcgccgt agtacttttt tgcgcagtta tatgtatgat
    36061 ttcattcagc gttttgcacc gcatttaacg cgtgatgtcg ttgatgcggc tgtcgcattg
    36121 cgctccaatg aagaaattga ggtcatgttt aaagatataa aactgccgga aaaataattt
    36181 cttgcgttat tttcggcacc ttttatgtag cgaaggtgcc ggaatatatt ctcttttgtt
    36241 acttatttct ataccgtgca caatattttt atccctcata tttatagggt aaattacctg
    36301 taaagtagtg tctaattacc cgcctcgcaa attccagttt tcaaatatct atttcaggtc
    36361 aaaagattga attattcact ggagacgatt cgtaaattcg ctggattttt gactaaagtt
    36421 tctttaggat ttgtctcatc gtatgattaa ttacaccaat cattcaacgt cttgctgata
    36481 agtgatggcg attatatgag gttagcaatg ccttctggaa atcaggaacc ccgccgtgat
    36541 cctgaattaa aacgtaaagc ctggctggcg gtttttcttg gttctgcact tttctgggtg
    36601 gttgtcgcac tgctgatttg gaaagtgtgg ggataactat ggtcggtcag gagcaactgg
    36661 agtcgtcacc attatgccag catagtgaca atgaaccaga aacgaaacgg gaatgttccg
    36721 tcgttattcc agacgactgg caactaacat cgcagcagca agcctttata gaactgtttg
    36781 ctgaagatga tcagccgaaa caataattat catcattctt attacccatt tttaatgaat
    36841 taaagggctt ttaatacacc gcagcaataa cagcttgagt tatctcaaca caaaataata
    36901 accgttaagg gtgtagccta tgatcaacac aaatatgaaa tattggtcct ggatgggcgc
    36961 gttttctctg tcgatgctct tctgggccga actcctctgg atcattactc actgatcctt
    37021 gaccccgctg cggcggggtt gtcatttgct ttgccacaag gtttctcctc ttttatcaat
    37081 ttgggttgtt atcaaatcgt tacgcgatgt ttgtgttatc tttaatattc accctgaaga
    37141 gaatcagggc ttcgcaaccc tgtcattaag gaggagctat gtcgtcaacc ctacgagaag
    37201 ccagtaagga cacgttgcag gccaaagata aaacttacca ctactacagc ctgccgcttg
    37261 ctgctaaatc actgggcgat atcacccgtc tacccaagtc actcaaagtt ttgctcgaaa
    37321 acctgctgcg ctggcaggat ggtaactcag ttaccgaaga ggatatccac gcactggcgg
    37381 gatggctgaa aaatgcccat gctgaccgtg aaattgccta ccgcccggca agggtgctga
    37441 tgcaggactt taccggcgta cctgccgttg ttgatctggc ggcaatgcgc gaagcggtta
    37501 aacgcctcgg tggcgatact gcaaaggtta acccgctctc accggtcgac ctggtcattg
    37561 accactcggt gaccgtcgat cgttttggtg atgatgaggc atttgaagaa aacgtacgcc
    37621 tggaaatgga gcgcaaccac gaacgttatg tgttcctgaa atggggaaag caagcgttca
    37681 gtcggtttag cgtcgtgccg ccaggcacag gcatttgcca tcaggttaac ctcgaatatc
    37741 tcggcaaagc agtgtggagc gaattgcagg acggtgaatg gattgcttat ccggatacac
    37801 tcgttggtac tgactcgcac accactatga tcaacggcct tggcgtgctg gggtggggcg
    37861 tgggtgggat cgaagcagaa gccgccatgt taggccagcc ggtttccatg cttatcccgg
    37921 acgtagtggg ctttaaactt accggaaaat tacgtgaagg tattaccgcc acagacctgg
    37981 ttctcactgt tacccaaatg ctgcgcaaac atggcgtggt ggggaaattc gtcgaatttt
    38041 atggtgatgg tctggattca ctaccgttgg cggatcgcgc caccattgcc aatatgtcgc
    38101 cagaatatgg tgccacctgt ggcttcttcc caatcgatgc agtaaccctc gattacatgc
    38161 gtttaagcgg gcgcagcgaa gatcaggtcg agttggtcga aaaatatgcc aaagcgcagg
    38221 gcatgtggcg taacccgggc gatgaaccga tttttaccag tatgttagaa ctggatatga
    38281 atgacgttga agcgagcctg gcagggccaa aacgcccaca ggatcgcgtt gcactgcccg
    38341 atgtaccaaa agcatttgcc gccagtaacg aactggaagt gaatgccacg cataaagatc
    38401 gcctgccagt cgattatgtt atgaacggac atcagtatca gttacctgat ggcgctgtgg
    38461 tcattgctgc gataacctcg tgcaccaaca cctctaaccc aagtgtgctg atggccgcag
    38521 gcttgctggc gaaaaaagcc gtaactcttg gcctcaagcg gcaaccgtgg gtcaaagcgt
    38581 cgctggcacc gggttcgaaa gtcgtttctg attatctggc aaaagcgaaa ctgacaccgt
    38641 atctcgacga actggggttt aaccttgtgg gatacggttg taccacctgt attggtaact
    38701 ctgggccgct gcccgatcct atcgaaacag caatcaaaaa aggcgattta accgtcggtg
    38761 cggtgctgtc cggcaaccgt aactttgaag gccgtatcca tccgctggtt aaaactaact
    38821 ggctggcctc gccgccgctg gtggttgcct atgtgctggc gggaaatatg aatatcaacc
    38881 tggcttctga gcctatcggc catgatcgca aaggcgatcc ggtttatctg aaagatatct
    38941 ggccatcggc acaagaaatt gcccgtgcgg tagaacaagt ctccacagaa atgttccgca
    39001 aagagtacgc agaagttttt gaaggcacag cagagtggaa ggaaattaac gtcacacgat
    39061 ccgataccta cggttggcag gaggactcaa cctatattcg cttatcgcct ttctttgatg
    39121 aaatgcaggc aacaccagca ccagtggaag atattcacgg tgcgcggatc ctcgcaatgc
    39181 tgggggattc agtcaccact gaccatatct ctccggcggg cagtattaag cccgacagcc
    39241 cagcgggtcg atatctacaa ggtcggggtg ttgagcgaaa agactttaac tcctacggtt
    39301 cgcggcgtgg taaccatgaa gtgatgatgc gcggcacctt cgccaatatt cgcatccgta
    39361 atgaaatggt gcctggcgtt gaagggggga tgacgcggca tttacctgac agcgacgtag
    39421 tctctattta tgatgctgcg atgcgctata agcaggagca aacgccgctg gcggtgattg
    39481 ccgggaaaga gtatggatca ggctcaagcc gtgactgggc ggcaaaagga cctcgtctgc
    39541 ttggaattcg tgtggtgatt gccgaatcgt ttgaacgaat tcaccgttcg aatttaattg
    39601 gcatgggcat cctgccgctg gaatttccgc aaggcgtgac gcgtaaaacg ttagggctaa
    39661 ccggggaaga gaagattgat attggcgatc tgcaaaatct acaacctggc gcgacggttc
    39721 cggtgacgct tacgcgcgcg gatggtagcc aggaagtcgt accctgccgt tgtcgtatcg
    39781 acaccgcgac ggagttgacc tactaccaga acgacggcat tttgcattat gtcattcgta
    39841 atatgttgaa gtaacaagta tttgcttgcc ggttattttg cttccggcaa gcaaatgaat
    39901 tacacaatgc aagagggtta tttgttcagc aaatggccca ttttctcggc tttggtatcg
    39961 agataatgtt cgttattggg gttacgacct acaatcaatg gtacgcgttc aacaatatta
    40021 atccctgctt cggtcagaat ttcgactttt ttcgggttat tggttaacaa gcggacttca
    40081 ttgacgccaa ggagtttgaa catatcagcg caaagagtga agtcgcgctc atcagcggcg
    40141 aagcctaact ggtggttagc ctctacggta tcgtaacctt gatcctgcag tgcgtaagcg
    40201 cggattttat tcagcagacc aatgttacga ccttcctgac ggtgatacag caaaatacca
    40261 cggccttcct cggcaatttg cgtcaatgcc gcttcgagct ggaagccaca atcgcagcgc
    40321 aagctgaaca gggcgtcacc ggtcagacat tcggaatgga cgcgcgcaag taccggggta
    40381 tgcccggaaa tatcgccata gactagcgcg atatgatcgt gtccggttgc cagttcttca
    40441 aatcccacca tcaggaaatc gccccatggg gttggcagtt tggcttctgc cacacgttta
    40501 agctgcatga aattctccag ataatgctgg ttctgtattg gcttattttg ccataacgag
    40561 aaggggttca cctaatcacg acgcgtcgat cgttcacgga atggcacaaa tctgtcaatt
    40621 tttcttggaa ctggcatttt cagttatgat tgtgggactt atcaaaaagg agaggccatg
    40681 cgttcgattg cccgacgtac cgcagtggga gctgcactat tgcttgtcat gccagtagcc
    40741 gtatggattt ctggctggcg ttggcaacct ggagaacaaa gttggctact aaaagcggct
    40801 ttttgggtta ctgaaactgt cacccagccc tggggcgtca ttacacattt gattttgttc
    40861 ggctggtttc tctggtgtct gcgttttcgc attaaggctg cctttgtatt atttgccatt
    40921 ctggcggcgg caatccttgt tggacaaggc gttaaatcct ggatcaaaga caaagtccag
    40981 gaaccacgac cttttgttat ctggttggaa aaaatacatc atattccggt tgatgagttc
    41041 tacactttaa agcgagcaga acgcggaaat ctagtaaaag aacagttggc tgaagagaaa
    41101 aatattccac aatatttgcg ttcacactgg cagaaagaga cggggtttgc ctttccttcc
    41161 ggtcacacga tgtttgctgc cagttgggca ctgctggccg ttggtttgct gtggccgcgt
    41221 cggcgaacgt taaccattgc tatcttgttg gtctgggcaa cgggagtcat gggaagccgc
    41281 ctgctgctcg ggatgcattg gccacgcgat ctggtggtag ctacgttgat ttcgtgggcg
    41341 ctggtggcgg tggcaacgtg gcttgcgcaa cgaatttgtg ggccattaac accacctgcg
    41401 gaagaaaatc gcgaaattgc acaacgagaa caagaaagtt agcgctggtt gattttccga
    41461 atttaggcct taaatcatca acaatgcgcg tggatgccat ttcgcagacg gcgcgaaaat
    41521 ggtactttaa agggctattg cggtaagttg accataattt attcgctcta accacataac
    41581 gggaagtaat gtgaaatatt tactcatttt cttactggtg ttagcgatct tcgtgatttc
    41641 ggtcacgttg ggtgcgcaga acgatcaaca ggtgacgttt aattatctgt tagcgcaagg
    41701 ggagtaccgt atttccacat tgctggcggt attgtttgct gcggggtttg ctatcggttg
    41761 gttgatttgt ggcctgttct ggctgcgagt tcgtgtttcc ctggcgcgcg ctgaacgtaa
    41821 aataaagcga ctggaaaacc agctttcacc cgcgactgac gtggctgtag tgccgcactc
    41881 gtcagcggcg aaggaataac tttctatgct ggagttgttg tttctgcttt tgcctgtagc
    41941 cgctgcctat ggctggtata tgggccgcag aagtgcgcaa caaaacaagc aagatgaagc
    42001 caaccgcttg tcgcgtgatt acgtagcggg ggttaacttc ctgcttagta atcaacagga
    42061 taaagcggta gacctgtttc tcgatatgct taaagaggat acaggcaccg ttgaagccca
    42121 ccttacgctc ggaaacctgt tccgttcgcg tggcgaagtt gatcgcgcta ttcgcatcca
    42181 tcagacccta atggaaagcg cctcgctgac ctatgaacag cgtctgttgg cgattcaaca
    42241 actggggcgt gattacatgg ccgccgggtt atacgaccgc gcggaagaca tgttcaatca
    42301 gctgaccgat gaaactgact tccgcattgg cgcgctgcaa cagttgctac aaatctacca
    42361 ggctaccagt gagtggcaga aagcaattga tgttgccgaa cgcctggtga agctgggtaa
    42421 agataaacag cgcgtcgaaa ttgcccattt ctactgtgag ttagccctgc agcatatggc
    42481 cagcgacgat ctcgatcgtg ccatgacctt gctaaaaaaa ggggcggcgg cagataaaaa
    42541 cagcgcccgc gtatccatca tgatgggacg cgtgtttatg gcgaaaggag aatacgccaa
    42601 agccgtcgaa agtttgcaac gcgtcatatc ccaggacaga gaactggtca gcgaaacgct
    42661 ggaaatgttg caaacctgct atcagcagtt gggtaaaact gccgaatggg cagaattcct
    42721 gcaacgcgcg gtggaagaga acaccggtgc cgatgctgaa ttgatgcttg ctgatatcat
    42781 cgaagcgcgc gacggtagtg aggctgcaca ggtctatatt acacgccagc ttcagcgtca
    42841 tccgaccatg cgtgtattcc ataagctaat ggattaccac ttaaatgaag cggaagaagg
    42901 gcgcgccaaa gagagtctga tggtgctgcg tgacatggtt ggcgagaagg tgcgtagtaa
    42961 gcctcgttat agctgccaga aatgtggttt taccgcatac accctctact ggcattgtcc
    43021 gtcttgtcgt gcctggtcaa ctattaaacc gattcgcggt cttgatggcc tgtaattttt
    43081 aaaaaaatcc gactttagtt acaacatact agttattgat gtcccattgt gctccggcaa
    43141 cgacggagca gaaaaagcct gctaggggaa aaatcgcaac tgttaatttt ttatttccac
    43201 gggtagaatg ctcgccgttt acctgtttcg cgccacttcc ggtgcccatc atcaagaagg
    43261 tctggtcatg acgttaactg cttcatcttc ttcccgcgct gttacgaatt ctcctgtggt
    43321 tgttgccctt gattatcata attgtgatga cgcgctgtcc tttgtcgaca agatcgaccc
    43381 acgcgattgt cgtctgaagg tcggtaaaga gatgtttaca ttgtttgggc cacagtttgt
    43441 gcgcgaactt caacagcgtg gttttgatat ctttcttgac ctgaaattcc acgatatccc
    43501 caacactgca gcgcacgctg tcgctgctgc agctgactta ggcgtgtgga tggtgaatgt
    43561 tcatgcctcc ggtggggcgc gtatgatgac cgcagcgcgt gaggcactgg ttccgtttgg
    43621 caaagatgca ccgcttttga ttgctgtgac agtgttgacc agcatggaag ccagcgacct
    43681 ggccgatctt ggcgtgacac tgtcacctgc agattatgca gaacgtctgg cagcactgac
    43741 gcaaaaatgt ggccttgatg gtgtggtgtg ttctgctcag gaagctgtgc gctttaaaca
    43801 ggtattcggt caggagttca aactggttac accgggcatt cgtccgcagg ggagtgaagc
    43861 tggtgaccag cgccgcatta tgacgccaga acaggcgttg gcggctggtg ttgattatat
    43921 ggtgattggt cgcccggtaa cgcaatcggt agatccagcg cagacactga aagcgatcaa
    43981 cgcctcttta cagcggagtg catgatgagt gattccaaca gccgtctggt ctactcaacg
    44041 gaaaccgggc gtattgatga gcccaaagcg gccccagtac gccctaaagg cgatggagcg
    44101 gtgcgtattc agcgtcagac cagtggacgt aaaggtaagg gcgtttgcct gattaccggt
    44161 atcgatctcg atgatgccga actgacaaaa cttgcagcgg aactgaagaa aaaatgcggc
    44221 tgcggcggag cagttaaaga tggagttatt gaaatccagg gcgataagcg tgatttatta
    44281 aaatcactac ttgaagcgaa agggatgaag gtaaaactcg caggcggtta acataaaaag
    44341 ccacggatat atccgtggct ttcgaatatt ttactgtgtg tattattcag ttatttcgta
    44401 ctgaacagaa tcaagcgtat tataattgcg agtcgataat cgacatttat ttacgattat
    44461 ttaccgacct ggtgaccaat aacaccaccg acagctgcac cacctaatgt acccaacgta
    44521 ctgccatcgg tcagtactgc accgcctaat gcccctgcac ccgcgccgat tgcggtgttg
    44581 cggtcccgtt tagaccagtt agaacaggca ctcagagaca ttgccaaagt aattgccaga
    44641 acagccgcgg tcattttttt gctcgttaca aacataatac tctctcctga atttatgatt
    44701 cacggaagta agctctctat aactataata gctaagaata agcctggtga aattattccg
    44761 tgaaatctgc tcgcgcgcag ggttatatca cgcaggtgat aattacttcc tgttatatcg
    44821 ctgataataa ttttatatct tgagagtgtt aataacaggt aaatagtctt aattatcaac
    44881 caggaatcat cttagagcgg atgatttgcc aaactgcaaa tcatccgtag agaagggaaa
    44941 tggttaaatg tcaacgacgt gaacggtgag tttactgtgc tcaagatgca tcagatcgct
    45001 ggcgcgtatt ttcgaatcgg taatcacgcg attgaatcgc tcaacgggac cgatggagta
    45061 tgaatgtaca gcaccaaatt tcgagctgtc agtcaggact atcgcttcgc actctttttc
    45121 cagcacggca ttgaccacat cggtgcgcat catatcgcga ccggtgaatc cagtttcagg
    45181 ttgccagcca tcaataccaa taaatgcttt gctgaaatgt acctgctgga tgcactggcg
    45241 tgtcaaaggg ccaaccatac tttcgctttt tttctggtac acgccaccga gcaaaatgac
    45301 ttcacaaggc gcgtctttca gcaaatgcgc gatgtagctg ctgaccgtga tgatagtgac
    45361 atttttcttc tgctccccca gagtccgggc gagcagggca ttgctgctgc cattttcgat
    45421 aaagatggtt tcgcccggtt gaaccagtga cgcggcaaac tcggcgagtt cacgcttcag
    45481 cgtatagttg ctcatcatac gggtttcgac gtcatcacta tcaagcgaaa ctgcaaagcc
    45541 atgtgcacgg cggaggtaac tcagtttttc gagggtgttg agatcctggc gaatggtaac
    45601 ttcagaaact ccagtggctt ttgccagatc ggttacgcta acctgaccct ggtcaatgac
    45661 catctgtaga atagtttgtt gtcgggaatt catcgctgta attcttataa cgttataata
    45721 ctaaataaaa aaatactgac gcgccgggca tcctcaaata ggaaaacctg aaggcacgat
    45781 tgttaaatct cccacggtgt cttcgggatg tctggcgtag attcagcaac ctggcgcaag
    45841 agttctgctt taagtatctc gagattatgg atcgcagagt ggtaatcgcc tgccacaagg
    45901 atatctaaca caatatcaat acgttcggca acgcgctggg catcaaattc agacatcaag
    45961 gacatcctta tagcaaaagg gggaaaatga ataatgcaaa aattgccgct aaaagagaag
    46021 tgtttaacag caacggctaa ttatcatcca ggaatacgat atataatgac gggatatagc
    46081 gctaagtata tatattcatc tacttatgcg cgcttcagat agcatttata ccagcgttcg
    46141 aaggcgacgg cgggcatcgg tttggcaaac aaaaatcctt gccgctcatt gatcccgttc
    46201 ttggttaaaa aagcatcttc ctttgcactc tctacacctt cggcgatcac ctgaagattc
    46261 aatgcctggg ccacagcgac gatcgcccgg accagtgact gcgagacagg ttgtttgtga
    46321 atatctcgaa caaaaacctg gtcaagtttg atggcatcga tcggaaagcg cgccagttgc
    46381 gaaagtgaag agtagccggt accaaaatca tccagatgca cttgcgcacc tagttggcta
    46441 aattgttgaa taacagacag tgccagttca tcattctcaa tcagacaact ctctgtcagt
    46501 tcaacatcta tagggcagta ttcaaaattg agttcctgga gaacctgttt cagggcggtg
    46561 aaaatggttt gatcggcgag ctgacgtgca gaaatattta ccgccactcg caggtttatg
    46621 cctttatccc gccactttgc cacctggcgt acgacatcga gaatcaccca acggcctaaa
    46681 ggcacaatta gccctgactc ttcggcgtag gaaatgaagt ccaacggtgg aatcaaccca
    46741 cgttcaggtg actgccaacg tactagtgct tccagactgc gcacttcgcc acgctaggtg
    46801 attttcggtt gatagtgaat aaccaactga tcgttttcca gtgctttacg caagttggta
    46861 tccagccaga gatattcaaa tacccgctga ttcatttctg gagtaaaaac gcaaaattgt
    46921 cctcgtccgc cttccttcgc tgtgtacatt gctgtgtcgg cgtgacgaat aatagtcgcg
    46981 ctgtccgaac catgttcggg agagagcgaa atacctactg aacagctggt ataaacttca
    47041 attaaaccaa tacgaaaggg gaggcgtaag cgggtcaaaa ttcgtgatgc cattgcttcc
    47101 agcgcgcttt gtgaggtatt ggatgccagt accagaaact catcgccacc tggacgcgcc
    47161 aacacctggt catgttcgag acagcttaaa atagccaatg acacgtcgcg taataactgg
    47221 tcaccaaaca aatgcccata ggcgtcgttg acctttttaa aattatccaa atcaagatac
    47281 acaaccccaa ctttattgtt atctgcctga ttaatagcgt gatcgattaa ctcctgcatt
    47341 gcgttacgat tcggcagtcc ggtgatactg tcggtatttg ccagaatacg cagtcgctcc
    47401 tgagtgcggc gctcttcggt aatgtcggta ccggaacaga ttaaaaaaat ctcgtttttg
    47461 ccactgccgc tgtggacaaa tttattgcga aacagaaaca gccgctggcc tttacgtgtt
    47521 ggtatccaca gttcgacttc atatgcattg ccgcttcgaa aaaatacacg gttattgcgc
    47581 ctggatgccg cagcttcacg acggctcata aacagtttaa acacgctttg cccaatgacg
    47641 tcgtgttctt tcaaccctgt gtaatcttca cataaccgat tgaagcgttg gatattcccc
    47701 cggctgtcga gaatcactat tgcggaatgt gcttcagaaa ctacctgctc agcaaaggaa
    47761 agcccgtgtg ataaatcacg agcaaccgcc ggtgtgtcat gccatgcaga cgcgttgcca
    47821 gcccattccc gtttattgat ttttcgtcct accagatgca cagaaagatc gctttcatcg
    47881 acggtcagac tcatcatcag gctggaggtg atgaccgtca tttccctgat gcgagcagcc
    47941 tgttcggcag ataactgcaa tgtacgatca ggttctgtgg tttcggtggt agaaaagcgc
    48001 aaagcatcgc tgctttccgt caaccgccag tatggattgt gcgatccgag aaagttgtac
    48061 aacgttgtgg actccctaac ggttttcatg atgcatatcc tccggttaac agcggagtcc
    48121 gcggttaaat aaaaggaaca acatgactta catgaaatta acggcggcta aacgcattgt
    48181 tttttatata tttatttgta atccagtttt ggaaaaacgc cagttttcaa acgaaagtca
    48241 gttaaaaaat ctgcctggat ataacgaagg tagagcgggg aaataaacgg cccatccatg
    48301 aggaatgggc cgtgaaagga gattacgcga ccgggcgcgc aataatgctg cgggtttcca
    48361 tgcggacttc ggcaatggtg acgtcaataa cgtcagttac tttgtaaacc gtttcacctt
    48421 taatttgtac agtgccgttt tcctggctgc aaaccagttc atcgcgcaca gcgtgtaaga
    48481 aaggtgccgg aataaaggcg atagcgccgt tatcaaccaa acgcacacgc atgccgccac
    48541 ggctgatatc gacaatttcc gccgcgaaac gggtgccggt cccggcttta tctttcagga
    48601 agcgtgcgta taaccagtcg ccaacatcac gttctgccat ccggttgaga cggcgacgtt
    48661 cggccatttg gacagtgatt tcatcctgcg gacgcgtcgc agtttcgcct ttgataaccg
    48721 ctttcagcag acggtggttg atcatgtcgc catatttacg gatcggcgaa gtccaggtgg
    48781 cgtatgcttc cagaccgagg ccaaagtgag gaccgggttc agtgctaatt tcagcaaatg
    48841 actggaagcg acgaatgcgg ctgtcgagga aaccagttgg ttgcgcatcc agttcacgac
    48901 gcagtttgca gaaaccgtcc agcgtgagca cttcttcggc atcgacatgc agaccgtgcg
    48961 ttttcagcaa cgctgccagc gcgtcggcat tcgccggatc aaagcccata tgcacgttat
    49021 agatgccaaa accgagctta tcgcgcagta cgcgggccgc acaaatgtta gcggcaatca
    49081 tcgcttcttc gacgatacgg ttggcaatgc gacgaggctc ggcgacgata tccagcactt
    49141 cacctttttc accgagaata aagcggtaat ccgggcgatc tttaaacacc agtgcgtggt
    49201 tatgacgcca ctcgccgcgg cgttggcaaa tttgcgctag caaacggact tgttcggcaa
    49261 ttgcttcact ttcaggcttc cagtcaccgg tattttccag ccagtcagaa acctggtcat
    49321 acaccagctt cgctttggat tcgatggtgg cggcaaagaa ttcgatatta tcttcaatgg
    49381 tgccatcagc ggagagcgtc atgcggcatg ccagtaccgg gcggacttca ttggcgcgca
    49441 gtgagcagag atcgtcagaa agctcgcgag gcagcatagg gatgttgaag ccaggcagat
    49501 agttggtgaa tgcgcgaatt ttcgcggctt tgtccagctt gctgccttca gcaatccacg
    49561 cggttggatc ggcaatcgcc acaatcagct gaagtttgtc atccggcagc gccttagcga
    49621 aaagggcgtc atccatatct tcagtgctgg cactgtcgat ggtgacaaaa tccagcgagg
    49681 tcagatcttc acgaaccaga ccttcatcga gcatttcggt agcgacgccg tctggtgctt
    49741 ctttttccag attatggcgc gcaagggtaa cccaccacgg tacaaagtgg tcgtcaccaa
    49801 aagtgatgta ttgtgtcagt tcagcataga aagaacgatc gcctttcagc ggatgacggc
    49861 gcatttcggc aaccgcccag tcgccttctt taaactcgtg gttcaggcca cgggctgcgc
    49921 ggcaaggaat ggcgtctttt aaaagtggat gatcaggaac gatggtcaga cggtcatttt
    49981 tgccctgaac tttacccacg aaacgagtca ggaacggttc aaccagttct tctggctctg
    50041 cggattcacg ttctttttca ctgtggatca ccgcgataat tcggtcgcca tgcatgactt
    50101 ttttcatctg cggcggcgga atgaaataac ttttttgcgc gtcgacttcc aggaagccaa
    50161 agcctttttc tgtggctttt accacccctt cagcgcgtgg cgtctgggaa tgcagttgct
    50221 gtttaagctg cgctagcagc gggttgtcct gaaacataat tgtctatttt ggtggccatt
    50281 agagcggctg acagttttac gcgaatctgt ctgacgcggc aaggttaata tgtctcaccc
    50341 aacgcgattt ttaagcgatt tatccagcca cacagcccgc tccataccag cagattaata
    50401 atctgcgttg atgatttttc gtgttcgagt aaagggctga actgggcggc gctaaagcga
    50461 tcgggtgctc gggtcagcca ttgtatcgcc tgaacggtag cgcgctcaac agaatgacgg
    50521 tcagcccatc ggttaatttc attttcgccc ttgcgcagaa gggtgctgat ttttttatat
    50581 tcatttgatg aatccataca ggtggaaaaa caagcaggac tgccatttat acgcgcgcat
    50641 aacaacgccg caagctgatt atccggctgt gtattgaccc gtgtgctgtt taacaacttt
    50701 tcgagaaggg aaagtgaaat agggtggaga gagagtgttt cggccagttg gcaaagttct
    50761 gcagttgaac tttttgtgtg ctcttcaggt agtttttcca cctcatagct gcttcgccag
    50821 cgtattgatt catcagcaaa cagtgacgcg tcggcgtagt tttgtatcgc cagcccgggt
    50881 aaccagcgta ccggatgccc gagataagcc tgaaatgtcg caattgtccg cgcctgaaag
    50941 ccaataaaac caatgatttg gttaatgatc acacagtccc agacagtaag accgacgtca
    51001 ttcagatgct ggcgcgatcg gttgtcgata atagaaggcg agctagccag ttggcgggca
    51061 tattgcgtga tttgtgccaa ccgatgatta ctttccctgg aggaatcagg tccggggagc
    51121 ggcgtaagtc gcgccgaata gtagttacat aaccgctgga cgccgcaaac ctgagcaacc
    51181 gtcagggccg tcgacaagcg ttcataagcg gtgaaggttt gtgaacgggt cacggtaagc
    51241 tggtcaggga acaactccac tgcaagaacg cgcgcaggga ccagccatga aaggaagggg
    51301 gccagcgtct cttcatcaag gatcacgtcg agcagaaagg gatcgctgac ctttgcggct
    51361 tccgggacca gaggcagaac gtcatactcc gtagtactgg attgcgtttc atgataccag
    51421 tggcttttgc cggtgatgtg gcgttgttcc atgggcgctc cttggtcgta aaggaaatcg
    51481 ttatcctgac gcaaggcggg aaggggagaa agacggatcg gcgataacaa atatcagaaa
    51541 ggtataacag agataacggg cggcagaacg ccgcccatct ttaccaacag aacgattatt
    51601 tcagttcgag ttcgttcatt gcagcgatgc tgaaaccgcc gtcaacgtgg actacttcac
    51661 cggagatacc ggcagagaga tcggagcaca ggaatgccgc agagttaccc acatcttcaa
    51721 tagtaacggt acggcgaatc ggggtaacgg cttcgcaatg agccagcatt ttgcggaagt
    51781 ctttgatacc ggaggccgcc agagtacgga tcggaccagc agagatggcg ttaacacgca
    51841 caccttccgg acccatcgcg ttcgccatat agcgcacgtt cgcttccaga gacgcttttg
    51901 ccagacccat aacgttgtag ttcgggatag cgcgctcagc gccaaggtag gaaagggtca
    51961 gcagggcaga acccggattc agcatggagc ggcaagcttt tgccattgca acgaagctgt
    52021 aggagctgat gtcgtgggca attttgaagc cttcacgggt aacggcgtta acatagtcac
    52081 catccagctg atcgccaggt gcaaaaccaa tagagtgtac gaaaccgtca aatttcggcc
    52141 aaactttccc cagttcagcg aacatggtgt cgatgctggc atcttctgca acatcgcact
    52201 gcagaacgat gtcagaaccc aattgagcgg caaattcttc tacgcggcct ttcagtttgt
    52261 cgttctggta ggtgaatgcc agttcagctc cttcgcggtg catcgcctga gcgataccgt
    52321 aggcgatgga cagtttgctg gcaacaccgg ttaccagaat gcgcttaccg gaaagaaaac
    52381 ccatagcttt aatccttatt gttgatgctt gttgtgcctg aaaatcaggc gattcgtcgt
    52441 tttagtaaac agtacgaaca gataaacggt tattataatc aacctggctg tgagtagcta
    52501 tagttgccag gtccgaccgg agcaggctgc ggcagggggg gcgcttttcc ccttacccta
    52561 accctctccc cagaggggcg aggggaccat attgtgcaaa tattgttacc ccagcaacaa
    52621 acaggctcat acagccccta acccttttat ggcgatggct gggatggttc agaccttgcc
    52681 gaatattctc cagcactgtc tccatgtttt caccacaaca gctattcggc tcggtcggcc
    52741 ccctcgccct ttcagggtga gggtgaccgg cggttcagtt cctgcagaat aatctccagc
    52801 accgcctcct cattacaatc aatctcgtta ttccagaaac gcagcacggt ccagccctgc
    52861 gactcaagcc agctagtgcg cctggtatca taggcaactg ccaaatcatg ctgcccacca
    52921 tccagctcaa cgactacgcg cgccgagcag caagcaaaat cgagaatgta gctccccacc
    52981 ggatgttgac ggcgaaattt gaaatcacca aaacgtcggc tgcgaagata tcgccagagc
    53041 tttcgttcct gcaaagtgag attgcgtctt aaatcacgcg catttgattt tattttatcc
    53101 atcacctcat tctgacaaga tttaatctct tgtcagaatg aggtgaataa tctggaagga
    53161 ggattcagaa aattagcgat ctttacgcca cgcatccgct gtcaatgctt cgccaaagtg
    53221 cccggcaatc agccgtttgg tgagttcatg cagcggcgat gccagcacat ccgcggtgct
    53281 gcctcgctcg acaacctcgc cctgatgcat caccagcacc tggtcgctaa tgtgcttcat
    53341 cattccgata tgctgggtaa cataaatata cgaaatgccc tgtttttcct gtaactccag
    53401 catcagatta atcaactgcg aacgcatcga catatccagt gaagcgaggg cttcatcggc
    53461 aataatgact tttgggcgca atatcagcgc gcgcgccaga cccagacgct gtttttgtcc
    53521 gggtgccaac atatgcggat agtaactgac gtgatccggt agcagcccaa ccatacgcat
    53581 cgtttcaata atctgtttgc gacgctgttc cggttccagg tcggtgttca ggcgcagtgg
    53641 aaaatccaga atttgcgaga tacgttgacg gggattcaac gaggtcgaag gatcctgaaa
    53701 aatcatgcga atacgctgac tacggaagga ataatcgcca aaatgcagcg gatgatcgtc
    53761 aatcaataac tcgccgctgg taggttctat cattcccgcc agcatttttg ccagtgtgga
    53821 tttacccgaa ccattctcgc caataatcgc cagtgtctgg ccttcacgta gcgtaaagct
    53881 caagggtttt accgcttcta cggtctgacg acgaaaccaa ccggtccggt agcggaacgt
    53941 tttacttaaa ttacgcactt caagcagcgt ttcgatcatc tcactctttc tccatgttca
    54001 gcgggaaatg acaggcatag agatgatttt tcgcccccgt caaacgtggc gtcacaatgc
    54061 attctcgttg tgcatacggg caacgtggcc ccagacgaca accaatcggt aactgttcca
    54121 gcaatgggat agcgccgggt agagtattga ggcgactttt atgcggcatc gcgctgccga
    54181 agtctggtat cgcgcggatc agcgcctggg tataaggatg atgtggcatc gtcaccaact
    54241 ccttactcgg cgcggtttcc actgtttgac cgcagtaaag cacgttaatt ttatccgccc
    54301 attggctaag catttgtaag tcatggctga taagcaaaat agtggtattg ctgttttggt
    54361 tgagacgcgt cagcaggcga aagatttgcg cctgggttgt tggctccatt gagtttgtcg
    54421 gttcgtcagc aatcagcaga cgcggttgat tcgccagtgc aatggctatc atcactttct
    54481 gacattcacc ttcggtcaac tcatagggaa aactgcgcat cgcatctttg tgatctttaa
    54541 tccccacgcg gtgcagcagt tcaatggcac ggcgtttgcg ccagccaaaa cgctgccacc
    54601 aacggccttt ataggtccag gctgggatgt tttgcatcaa ctggcgaccc acacgttctg
    54661 aagggtcaag acacgactgc ggttcctgga aaatcatcga cacgttatgg ccaaccagtt
    54721 tgcgccgttc gcgtgcggag agacgcagca aatcgatatc atcaaaacgc atacggtcag
    54781 cagtaacacg ccagttatct ttattcaccc cacaaatcgc tttcgcaatc aaactcttgc
    54841 cggaaccgga ttcaccaaca agaccgcgga tttcaccttc ggttaaggtc atacttaccc
    54901 ggtcgacggc tttaacccac tcatcaccgg ttttaaattc aatggtcagg ttacgaatat
    54961 cgagtaatgg cattattcca cccccgcaat aatcgcacga cgaacgccat cgccaaggag
    55021 gttaaccaac aacacgctaa tcataattgc cgcacctggc agcatgacag tccacggggc
    55081 gacataaatc agttccagcg catcaccgag catcgctccc cattcaggcg aggggagttg
    55141 tgcgccgaga tcgagaaagc ccagcgcggc gatatcgaga attgccatcg acagtgcgcg
    55201 ggtgatctcg gtgaccagcc cggcggtgat gtttggcatc acagcaaacc agagaatatt
    55261 cagcgttgat gcgccatcca gacgggcggc gataacgtac tctttttcca gttcgtcatg
    55321 caccatgctg taaatcgaac gtaccatacg cggcagcaat gccagccaga cagcaaacat
    55381 agcgtgggac aaactcggtc cggcaaacgc tacaacgata attgccaaca gcagcgaagg
    55441 gatcgccagt aaggtatcca gaatatggtt aagcaccgct gagcggaggc cgtgtgtcgc
    55501 cccggcgaag gtgcccagta ccaggccaca gatcgtcgcg gcaagcgtca ccacaaatgc
    55561 gccacccacg gttggcgcag ctccgctcag taaacggctt aaaacatcac gtccgaggtc
    55621 gtcagtcccc aggaagaaag aaacttcgcc atagcgcgac catgatggcg gcaataattg
    55681 ataaccgaga aattgctggt cgatgccgta aggcgcaaac cagccgccaa aaatacacag
    55741 tacagccagt cccgcgcagc cgtacaggcc gaccatcgca gaggcgtcac tataaaattt
    55801 gcgccaggcg gtacgcagcg tgcccggcgg gcgcttttcg ctgtatacgc tatcgtaagg
    55861 cataccattc cttatgtttc agagggttag ccatggcacc caaaatatca gaaatcacgt
    55921 taacaataat gaccagtgag ccacacacca tcactccggc ggaaatggct gcgtaatcct
    55981 gctggcggat ggcgttaatt aaccagcgcc ctaaacccgg ccagctaaaa accatttcgg
    56041 tgatcatcgc cagcgtcagc atggtggaaa actgtaagcc caggcgagga ataaccggcg
    56101 gtaacgcgtt atgcagaacg tggcgacgca aaatagtaaa gcgcgacaaa ccgcgggtcg
    56161 ccgccgcttt tacatagttc tggtcataca cttcaatagt gctgatacgc atcaggcgga
    56221 tcacttcagt tgttggcgca accgacaggg tgatcacggg caatatcata tggcggattg
    56281 cgctcatgat catttcatcc cgccatggtg agtccgaaag ccaggcatca atcaacgcaa
    56341 aaccggtaat cggtttcact tcgtagagca gatcgaaacg ccctgaaacg ggcaaccaac
    56401 ctagcgtgag tgaacaaaac agtgtcaaca gaagcgccag ccagaacacc gggattgaaa
    56461 agcccagtaa ggcgatggcg ttgatcaagt tatcctgcca cttatggcgc gtaatcccgg
    56521 caatcatccc cacggggatg ccaacaatca gcgcaaaacc gaaggcgagg atgcacagtt
    56581 ctatcgtcgc cgggaagacc tcttttaact gttcggcaat tggttgaccg ttaatgctgg
    56641 aaacaccaaa atcccagtgg atcaggccat taaaccagaa cacccaggca ttccacagtg
    56701 acgcgccttg taacggcgcg tgtggagtga aatagctcaa gctgaagcca acgaaggtca
    56761 gcaggaacaa ggtgacaatc aataacaaaa tgcggcgtaa ggtgaagata atcatggttt
    56821 tttcacctca tcctgtttct cgcgatacac cccagcaaag gaggcgttac caaacgggct
    56881 aagtaccaga cctttgatat cgtaccgata ggcctgcaaa cgcaatgatg acgccagcgg
    56941 cagaataggt aattcctgcg ccagaatact ctgcgcttcg tcataggctt caatacgcgc
    57001 tgccagctgc tgcgaggaga gcgccttacg caacacgctg tcgaatttcg gatcgcacca
    57061 gtgggcgagg ttggtctgtg aatgaattgc cgcgcagctc agtaacggac ggaagaaact
    57121 gtccgggtcg ttactgtccg tcgcccaacc ggataacgtc agatcatggc tcatatccat
    57181 caaccgcgcc tcctgaaagc gaccttctac cggcacaatc accactttta cgccaacctg
    57241 cgccatatcc gcctgaatca gttcggcagt tttcagtgga ctggggttcc acgcctgcga
    57301 acgtgtgggc acccacagtt tcagcgttaa gttttccagc cccaacgcct tcaactgttc
    57361 gcgcgatttc gccggattgt attcagtaat tttagcctcg ttgtcatagg cccacgaggc
    57421 gcgcggtaaa atagaggccg ccgtttcagc cgtaccataa tagatggatt gcatcaggcg
    57481 ctggttatta atcgccagtg ccagtgcatg gcggacagcg ggattattta gcggcggttt
    57541 ggcggtgtta aatgccagat aggcgacgtt catcccagga cgcagcgtta aacgcaagcg
    57601 cgggtcgtca cgcaaaatgg atagctggct ggcagcaggc caggccagaa cgtcgcattc
    57661 cccggtcagg agtttcgaca gacgtccggt gccgccggag cctaaatcca ccactacctg
    57721 cggcattaac ggtttaccgc gccagaagtc atcatgacgt tgtaggcgaa taaattgccc
    57781 ggcgcggtat tccgacaact gatacggacc ggtgccgacc ggttgacggt cgagttgctc
    57841 ctggcgatct tctttctcta acttccgggc atattctgcc gacatgaccg aagcataatg
    57901 ggttgcgagg tgccacaaaa aagaagcatc cggctgggcg agtcggaact caacggtatg
    57961 attatccagt ttgcggacgc ttttcacgtt atcggcaaat tgcaggctgt cgaagtaggg
    58021 gaagttgctg ccgttgacgt tatgccacgg gttgttgcgg tcaaaaattc gctggaaagt
    58081 aaacaccaca tcgtcggcat tcattttacg agtgggagta aaccagtcgg ttttttgaaa
    58141 cggaacatcg cgacgcaggt ggaagcgata ggtcgcgccg ttgtcgagta cttcccagct
    58201 ttcggcaagt tccggcatca ggcgataggt atagggatcg acatccagca gtcgatcata
    58261 aaactgggcg gcaagggtat cgacaattaa cccactgctc gctttggatg ggttaaaggt
    58321 gttgacttgc ccgctgacgc aatagacaaa accgctgtcg cggatatcag catgcggggg
    58381 ggattcaggc gcggcgattg cctgaccact cacaagtcca gcaatcacca aaagagacga
    58441 taatacctgg cgcataatat taagggattt tatgtaaaga ggctatctta ctaatattta
    58501 atgacatttg ccattaccgt ttgtgttcag gggtcgtaat gagggccgtg tggttggtcg
    58561 ttgaacaggt ggcgtcgcca tctgttcaac attcgtaccc gtgatttctc tataactata
    58621 actcacagaa caacttagcg aggagcagag cggtacgatc gtgagaaacc cattaaagca
    58681 cgctgcagtg cgtgtcattg ttagccggat gcggcgtgaa cgctttatcc ggacaacgat
    58741 actgaccgat cgtctgcaat taaataatta ctcattaccc cattgattca aaaactctgc
    58801 gatcttatca atgcgtacgg gattaatccc cgcttcagca gccatttcat gttgggcttc
    58861 ttcgctgatc tcttcattgt tcatcaaacg ggtgagcagt aactggaagt agtgggccag
    58921 cggtgtgcgt tcagcctctg cgacaggctc tgcgcattcg gtcgcgtact catcggcaat
    58981 atcaaaatat tttagcggga tatcgtggtt cattatttgc ccctggggtt aatgcgctaa
    59041 gcataagggc ggatgatagc actcgtgatc cgggtcatac atctttttat agttaatggc
    59101 gcggcgcgtt accctcaggc aggataatgc gccgcgcatc cggctattac gtttcgctca
    59161 ccggcgttct gtcaccttcg tacagcggca ctttgcgata gcggcggatc agataccaca
    59221 aatatgcgcc gcctaacgaa gcccatacca gaccgagtgt cagtgacgtt gactctaggt
    59281 taacccacag cacacccacc gtcagcgcgc caaccagcgg catcagcaaa tagtggaagt
    59341 gatccttcca gcttttattc attcctttac gccgccagaa atgattaaac accgacagat
    59401 tcacgaaggt aaacgccacc agagcaccga agttaatcag tgctgtcgcg gtgactaaat
    59461 caaagaacag cgccgacagc gcgacaatcc cgaccataat gacgttgagt gccggagtcc
    59521 gccattttgg gtgcacatag ccaaacacgc gctccggaaa cacattgtca cgccccatca
    59581 catacagcag acgcgacacg ctggcatgtg aagccaggcc agacgctaat gtgttcacaa
    59641 acgtggtgca gaggaaaatc gactgaaaga gcttgccgcc gacgtacagc gcaatttcag
    59701 gcagtgcggc gtccgggtct ttaaagcggc tgatatcggg aaagaacagc tgcataaaga
    59761 acgacgccgc gataaagata acgccgccat ataccgccgt caggaagatg gctttcggga
    59821 tcacgcgtgc ggcgtccggc gtctcttccg aaagcgtggt caccgcatcg aaaccgagga
    59881 acgagaaaca gacaatcgtc gcaccggtaa taatcgggat caggtgcgcg ttctcgctga
    59941 taaacggctg aagtgaccag acggtgccaa cgccttctcc tttatgcagt ccctgaacca
    60001 ccaggaagat aaacaccacc atgatggaga tttgcaccag tacaaacagg gtattgaagt
    60061 tagcgaccag gttgacgctc ttcagattcg cggcggttaa aatggcgacg aaggttacca
    60121 cccacaccca cggcggcact tccgggaaga gggcggagag atagattttc gccaacaaaa
    60181 cgttgatcat tggcaaaaag agataatcca gcagtgatga ccagccgacc ataaatccga
    60241 cgtgcgggtt aatcgacttt tgcgcgtagg tataggccga accggcctcc ggaaactggc
    60301 gaaccagttt gccgtagctg atagcggtaa acagcacgcc cgccagcgcc agcaaatagg
    60361 acgccggaac gtgaccgtcg ctaatgccgg agacaatacc aaaagtatca aatacggtca
    60421 tcggcgtgag ataggccaga cccatcatta ccacctgcca caatttcagt gattttcgca
    60481 gacgggtttt gccgggttgc gcagcaatat tcagtggtga attaatagcc ataattgctg
    60541 cctcccctgc gtaataaggt ttgcggtcgt gtcgacctga aatcaaacct gtttagcaag
    60601 ggaagcaaag ggggcggagt ccagtcagta gcaggagggg cgcatacgca gcctccctcg
    60661 ccatgcgggg atttaatcat cggcatcatc tcatttcctc gtcacagtct taatcgttaa
    60721 cggaagcccg ttgccgcctg cgctccataa cgcgacatcc gggaaatgtc tggattacca
    60781 gcaaatgtca ggcctggctc cgctcaggcc gatgaaacaa ccccgcaagg ggaattacgc
    60841 gtttttcaac atccactcaa tttctgtttc tgtgatgagg cgctcaaact gcaacagctc
    60901 atcattttta caggcgtgat acacatggca aaaccgttcg cctaaatagc ggcgcaggtg
    60961 atcattctcg ataaactcac ccagggcatc gctctggcga atcgggaagg gtaagccttc
    61021 ctgttccagc ccgttgcctt cgacttcttc ctgcaacggc agctcgttat caaggccatg
    61081 caaaataccg gcaaaaatcg ctgccatcac cagatatggg ttggcatcgg cacccgccac
    61141 gtgatattcc acgcggtgat tatgacggtc gccgcacgga atacgcaggg cgacggtgcg
    61201 gttgttatgg ccccacgacg cctgcgtcgg cacatacatt cccggctgga agcggcgata
    61261 cgagttcacg tttggtgcca gcaacgccat cgacgacggc atcaggtcaa tcatcccggc
    61321 gagcatcttt ttcagcagcg gcgaatcttc gccttctgtg tcagaaagca cattctcgcc
    61381 acgattattt tgcatactga tatggatatg catcccgctg cccgcgtgct cttcatacgg
    61441 cttcgccata aaagtggcgt gcatcttatg cttttctgcc atcagacgca ccaggcgttt
    61501 tagtgccagc gcatcatcgc aggcttccag cacgttatca gtatggtaaa ggttgatttc
    61561 aaactgaccc ggcgaggcct cagcgaccgc gccatctgcc ggaatcagct gcaactgcgc
    61621 cagttcatca atatcattga gcacgtcggc gaagtggttg aggttatcaa cggagtaaac
    61681 ctggctttgt gtattgcggt catcggtgcc aggcgcgcag ggcggttgca gatccccttc
    61741 agcgtcgcgc cggcgatcca gtaaatagaa ctccagctct accgctacaa ccgggaacaa
    61801 tccgcgctgg cgcagctgct gccacaggcg gttaagaacg ttccgcggct caacgtcaaa
    61861 gggagcgcca tcttcatcga ccatggtcag gagcatctga ccgataaact ctggatcggc
    61921 ggcagaagga gttaaggaac cgagaacagg aacacaggta cgatccggct cgcccatttc
    61981 ctgacccaga cctgcttctt ctactacgtt gcccagaata tccattgcaa ataccgaggc
    62041 cgggaaataa taccctttct cgagcttctt caggcttgaa acagggatac gttttccacg
    62101 gaagcaacca ttcaaatcag taagtaaaac atcaacatat tgcgtattcg ggtagcgctc
    62161 caggtagcgt ttcacttcct gcgtaaaggc gctaccccgc ctctcttctg actgctgaac
    62221 aaagttctct acttcaacga tattggtttc catgattctt cgcctttggt ttgttttccg
    62281 ctcgttatca aagcgtaaaa tataatgacc atcattcgaa tctgtatgca aactaaatgt
    62341 ttgtcaaatg ttaaattgag tttgcaaaaa tgaaaaccca ctgctagatt gaaaaaatat
    62401 tgaacataaa ggtcatttaa agcgcagtaa tggcgataat ttagtccact ttgtgagatt
    62461 gagcatggaa aatataatga acaatccggt tatcggtgtc gtaatgtgca ggaacaggct
    62521 taagggtcat gcgacccaga ctctgcaaga aaagtacctg aatgccatca tccacgcagg
    62581 cggcttgcca attgcgctac cacatgcgct ggcggaaccg tcgttacttg aacaactttt
    62641 gccgaaactc gatggcattt atcttcctag tagtcccagc aatgtgcagc cgcacctata
    62701 tggtgaaaac ggcgatgagc ctgacgccga tcccgggcgt gatcttctga gcatggcgat
    62761 aattaatgcc gcactcgaaa ggcgcatccc cattttcgcc atctgccggg gtttacaaga
    62821 actggtggtg gcaaccggtg gttcgttgca tcgcaagctg tgcgaacagc ctgaattgct
    62881 ggaacatcgg gaagatcccg aactgccggt ggaacagcaa tatgcaccgt cgcatgaagt
    62941 tcaggttgaa gaggggggat tactgtctgc gttgttacct gaatgtagca acttttgggt
    63001 aaactctcta catggacaag gggcgaaggt cgttagccca cggttgcgtg ttgaagctcg
    63061 ctcgccggat ggcttagttg aggcggttag cgtcatcaat catccttttg cgctgggcgt
    63121 acagtggcac ccggaatgga acagtagcga gtacgcgctt tcgcgtatat tgttcgaggg
    63181 ctttatcacc gcttggcagc accatatcgc tgaaaaacag cgactctgac cactacagtt
    63241 taaggaaatg caaatatgag tgatgaggga ctggcgccag gaaaacgctt gtcggaaatc
    63301 cgccagcagc aggggctttc acaacgtcgt gccgccgaac tctccgggct gactcacagt
    63361 gctatcagta cgatagaaca agataaagtc agccctgcca tcagtacgct gcaaaagctg
    63421 ctgaaggtgt atggtctgtc actctcggaa ttcttttccg agccggaaaa acctgatgag
    63481 ccgcaggtcg tcattaatca ggacgactta attgagatgg gcagtcaggg tgtgtcaatg
    63541 aagctggttc ataacggtaa cccgaatcgc acgctggcga tgatctttga aacgtaccag
    63601 ccgggcacaa ccactgggga aagaattaag catcagggtg aggaaatagg cacagtactg
    63661 gaaggtgaaa ttgttctgac gattaatggt caggattacc acctcgtcgc ggggcaaagt
    63721 tatgccatta ataccggcat cccgcacagt ttcagtaata cgtcggcagg tatttgccga
    63781 attatcagcg cccatacgcc caccacgttt taatcttttt gttctgtaag ccgggtaagc
    63841 gcaatgcgcc cggcaatcca taaagaatgt cgccagaaat aaacgggcat gcagcccggg
    63901 gatctctgcg ccctgacgtt cacaaactgc atatatctga tagacgtgaa acaggagtca
    63961 taatgaattt tcatcatctg gcttactggc aggataaagc gttaagtctc gccattgaaa
    64021 accgcttatt tattaacggt gaatatactg ctgcggcgga aaatgaaacc tttgaaaccg
    64081 ttgatccggt cacccaggca ccgctggcga aaattgcccg cggcaagagc gtcgatatcg
    64141 accgtgcggt gagcgcagca cgcggcgtat ttgaacgcgg cgactggtca ctctcttctc
    64201 cggctaaacg taaagcggta ctgaataaac tcgccgattt aatggaagcc cacgccgaag
    64261 agctggcact gctggaaact ctcgacaccg gcaaaccgat tcgtcacagt ctgcgtgatg
    64321 atattcccgg cgcggcgcgc gccattcgct ggtacgccga ag
//
LOCUS       DADLSB010000005        63890 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.005, whole genome shotgun sequence.
ACCESSION   DADLSB010000005 DADLSB010000000
VERSION     DADLSB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.005"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..2565
                     /locus_tag="JN331_000307"
     CDS             <1..2565
                     /locus_tag="JN331_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HAY7258546.1"
                     /translation="SGGVPSFIGGLVVFVSAAFNAQAETWFDPAFFKDDPSMVADLSR
                     FEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAF
                     PAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGI
                     NALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEW
                     KNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVV
                     RGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYS
                     VPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGPWGTTWYGGGQYA
                     EYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQ
                     LMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQ
                     QLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQV
                     FALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLS
                     YSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQG
                     ITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASL
                     KRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTN
                     SGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
     gene            2556..3626
                     /locus_tag="JN331_000308"
     CDS             2556..3626
                     /locus_tag="JN331_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7258547.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLEARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            3638..4180
                     /locus_tag="JN331_000309"
     CDS             3638..4180
                     /locus_tag="JN331_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7258548.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4188..4703
                     /locus_tag="JN331_000310"
     CDS             4188..4703
                     /locus_tag="JN331_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7258549.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4669..5406
                     /locus_tag="JN331_000311"
     CDS             4669..5406
                     /locus_tag="JN331_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY7258550.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5517..6527
                     /gene="pyrD"
                     /locus_tag="JN331_000312"
     CDS             5517..6527
                     /gene="pyrD"
                     /locus_tag="JN331_000312"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY7258551.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6701..7243
                     /gene="zapC"
                     /locus_tag="JN331_000313"
     CDS             6701..7243
                     /gene="zapC"
                     /locus_tag="JN331_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY7258552.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7240..8349)
                     /gene="ycbX"
                     /locus_tag="JN331_000314"
     CDS             complement(7240..8349)
                     /gene="ycbX"
                     /locus_tag="JN331_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY7258553.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8593..10701
                     /gene="rlmKL"
                     /locus_tag="JN331_000315"
     CDS             8593..10701
                     /gene="rlmKL"
                     /locus_tag="JN331_000315"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY7258554.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWAVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10713..12620
                     /locus_tag="JN331_000316"
                     /pseudo
     CDS             10713..12620
                     /locus_tag="JN331_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            12750..14003
                     /gene="pqiA"
                     /locus_tag="JN331_000317"
     CDS             12750..14003
                     /gene="pqiA"
                     /locus_tag="JN331_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY7258555.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLMTILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGIGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14008..15648
                     /gene="pqiB"
                     /locus_tag="JN331_000318"
     CDS             14008..15648
                     /gene="pqiB"
                     /locus_tag="JN331_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY7258556.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15645..16208
                     /gene="pqiC"
                     /locus_tag="JN331_000319"
     CDS             15645..16208
                     /gene="pqiC"
                     /locus_tag="JN331_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY7258557.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16464..16631
                     /gene="rmf"
                     /locus_tag="JN331_000320"
     CDS             16464..16631
                     /gene="rmf"
                     /locus_tag="JN331_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY7258558.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16701..17219)
                     /gene="fabA"
                     /locus_tag="JN331_000321"
     CDS             complement(16701..17219)
                     /gene="fabA"
                     /locus_tag="JN331_000321"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY7258559.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17288..19048)
                     /locus_tag="JN331_000322"
     CDS             complement(17288..19048)
                     /locus_tag="JN331_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY7258560.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19233..19685
                     /gene="matP"
                     /locus_tag="JN331_000323"
     CDS             19233..19685
                     /gene="matP"
                     /locus_tag="JN331_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY7258561.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19761..20807)
                     /gene="ompA"
                     /locus_tag="JN331_000324"
     CDS             complement(19761..20807)
                     /gene="ompA"
                     /locus_tag="JN331_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY7258562.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPSGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21163..21672)
                     /gene="sulA"
                     /locus_tag="JN331_000325"
     CDS             complement(21163..21672)
                     /gene="sulA"
                     /locus_tag="JN331_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY7258563.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21891..22520
                     /gene="sxy"
                     /locus_tag="JN331_000326"
     CDS             21891..22520
                     /gene="sxy"
                     /locus_tag="JN331_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY7258564.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22483..24645)
                     /gene="yccS"
                     /locus_tag="JN331_000327"
     CDS             complement(22483..24645)
                     /gene="yccS"
                     /locus_tag="JN331_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY7258565.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(24655..25101)
                     /locus_tag="JN331_000328"
     CDS             complement(24655..25101)
                     /locus_tag="JN331_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY7258566.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25224..27278
                     /gene="helD"
                     /locus_tag="JN331_000329"
     CDS             25224..27278
                     /gene="helD"
                     /locus_tag="JN331_000329"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY7258567.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27310..27768)
                     /gene="mgsA"
                     /locus_tag="JN331_000330"
     CDS             complement(27310..27768)
                     /gene="mgsA"
                     /locus_tag="JN331_000330"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY7258568.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(27864..28526)
                     /locus_tag="JN331_000331"
     CDS             complement(27864..28526)
                     /locus_tag="JN331_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY7258569.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            28699..29112
                     /locus_tag="JN331_000332"
     CDS             28699..29112
                     /locus_tag="JN331_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY7258570.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29157..29474)
                     /gene="hspQ"
                     /locus_tag="JN331_000333"
     CDS             complement(29157..29474)
                     /gene="hspQ"
                     /locus_tag="JN331_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY7258571.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(29532..30722)
                     /gene="rlmI"
                     /locus_tag="JN331_000334"
     CDS             complement(29532..30722)
                     /gene="rlmI"
                     /locus_tag="JN331_000334"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY7258572.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            30817..31095
                     /gene="yccX"
                     /locus_tag="JN331_000335"
     CDS             30817..31095
                     /gene="yccX"
                     /locus_tag="JN331_000335"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY7258573.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31092..31421)
                     /gene="tusE"
                     /locus_tag="JN331_000336"
     CDS             complement(31092..31421)
                     /gene="tusE"
                     /locus_tag="JN331_000336"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY7258574.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31512..32171)
                     /gene="yccA"
                     /locus_tag="JN331_000337"
     CDS             complement(31512..32171)
                     /gene="yccA"
                     /locus_tag="JN331_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY7258575.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32378..32465)
                     /locus_tag="JN331_000338"
     tRNA            complement(32378..32465)
                     /locus_tag="JN331_000338"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32429..32431),aa:Ser,seq:tga)
     gene            32891..34009
                     /gene="hyaA"
                     /locus_tag="JN331_000339"
     CDS             32891..34009
                     /gene="hyaA"
                     /locus_tag="JN331_000339"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY7258576.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34006..35799
                     /gene="hyaB"
                     /locus_tag="JN331_000340"
     CDS             34006..35799
                     /gene="hyaB"
                     /locus_tag="JN331_000340"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAY7258577.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALVIVQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            35818..36525
                     /gene="hyaC"
                     /locus_tag="JN331_000341"
     CDS             35818..36525
                     /gene="hyaC"
                     /locus_tag="JN331_000341"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY7258578.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36522..37109
                     /locus_tag="JN331_000342"
     CDS             36522..37109
                     /locus_tag="JN331_000342"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY7258579.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37106..37504
                     /gene="hyaE"
                     /locus_tag="JN331_000343"
     CDS             37106..37504
                     /gene="hyaE"
                     /locus_tag="JN331_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY7258580.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37501..38358
                     /locus_tag="JN331_000344"
     CDS             37501..38358
                     /locus_tag="JN331_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY7258581.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38492..40036
                     /gene="appC"
                     /locus_tag="JN331_000345"
     CDS             38492..40036
                     /gene="appC"
                     /locus_tag="JN331_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY7258582.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIVLVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40048..41184
                     /gene="appB"
                     /locus_tag="JN331_000346"
     CDS             40048..41184
                     /gene="appB"
                     /locus_tag="JN331_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7258583.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVASNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFSAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41196..41288
                     /gene="cbdX"
                     /locus_tag="JN331_000347"
     CDS             41196..41288
                     /gene="cbdX"
                     /locus_tag="JN331_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY7258584.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            41368..42666
                     /locus_tag="JN331_000348"
     CDS             41368..42666
                     /locus_tag="JN331_000348"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY7258585.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(42781..44947)
                     /gene="etk"
                     /locus_tag="JN331_000349"
                     /pseudo
     CDS             complement(42781..44947)
                     /gene="etk"
                     /locus_tag="JN331_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(44967..45413)
                     /gene="etp"
                     /locus_tag="JN331_000350"
                     /gene_synonym="yccY"
     CDS             complement(44967..45413)
                     /gene="etp"
                     /locus_tag="JN331_000350"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY7258586.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(45401..46540)
                     /locus_tag="JN331_000351"
     CDS             complement(45401..46540)
                     /locus_tag="JN331_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY7258587.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(46586..48682)
                     /locus_tag="JN331_000352"
     CDS             complement(46586..48682)
                     /locus_tag="JN331_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY7258588.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSIGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(48682..49428)
                     /locus_tag="JN331_000353"
     CDS             complement(48682..49428)
                     /locus_tag="JN331_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7258589.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(49425..50069)
                     /locus_tag="JN331_000354"
     CDS             complement(49425..50069)
                     /locus_tag="JN331_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7258590.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(50176..50481)
                     /locus_tag="JN331_000355"
     CDS             complement(50176..50481)
                     /locus_tag="JN331_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258591.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(50923..51135)
                     /gene="cspH"
                     /locus_tag="JN331_000356"
     CDS             complement(50923..51135)
                     /gene="cspH"
                     /locus_tag="JN331_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAY7258592.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            51421..51633
                     /gene="cspG"
                     /locus_tag="JN331_000357"
     CDS             51421..51633
                     /gene="cspG"
                     /locus_tag="JN331_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAY7258593.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            51644..51832
                     /locus_tag="JN331_000358"
     CDS             51644..51832
                     /locus_tag="JN331_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY7258594.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            51807..52037
                     /gene="ymcE"
                     /locus_tag="JN331_000359"
     CDS             51807..52037
                     /gene="ymcE"
                     /locus_tag="JN331_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HAY7258595.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNIPYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            52027..52200
                     /locus_tag="JN331_000360"
     CDS             52027..52200
                     /locus_tag="JN331_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAY7258596.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(52249..53322)
                     /locus_tag="JN331_000361"
     CDS             complement(52249..53322)
                     /locus_tag="JN331_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAY7258597.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(53424..56090)
                     /gene="torS"
                     /locus_tag="JN331_000362"
                     /pseudo
     CDS             complement(53424..56090)
                     /gene="torS"
                     /locus_tag="JN331_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309175.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            56218..57246
                     /gene="torT"
                     /locus_tag="JN331_000363"
     CDS             56218..57246
                     /gene="torT"
                     /locus_tag="JN331_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAY7258598.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(57219..57911)
                     /gene="torR"
                     /locus_tag="JN331_000364"
     CDS             complement(57219..57911)
                     /gene="torR"
                     /locus_tag="JN331_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAY7258599.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            58041..59213
                     /gene="torC"
                     /locus_tag="JN331_000365"
     CDS             58041..59213
                     /gene="torC"
                     /locus_tag="JN331_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAY7258600.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            59213..61759
                     /gene="torA"
                     /locus_tag="JN331_000366"
     CDS             59213..61759
                     /gene="torA"
                     /locus_tag="JN331_000366"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HAY7258601.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            61756..62355
                     /gene="torD"
                     /locus_tag="JN331_000367"
     CDS             61756..62355
                     /gene="torD"
                     /locus_tag="JN331_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY7258602.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIATLTVRDDARLELAADFCGLFLMTDKQVALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFAALCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(62448..62753)
                     /gene="cbpM"
                     /locus_tag="JN331_000368"
     CDS             complement(62448..62753)
                     /gene="cbpM"
                     /locus_tag="JN331_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY7258603.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(62753..63673)
                     /gene="cbpA"
                     /locus_tag="JN331_000369"
     CDS             complement(62753..63673)
                     /gene="cbpA"
                     /locus_tag="JN331_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY7258604.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGAPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT        15720 a        15825 c        16822 g        15523 t
ORIGIN      
        1 tctggtggag tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc agcgttcaat
       61 gcacaagctg aaacctggtt cgatcctgct tttttcaaag atgatccctc aatggtggcc
      121 gatttgtctc gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg tgtcgatatt
      181 gttctgaatc agacaattgt agatacgcgc aacgtcaatt ttgttgagat aacgccagag
      241 aaggggattg ccgcctgttt gacgactgaa agcctggatg caatgggtgt taatactgat
      301 gcgtttccgg cttttaaaca actggacaaa caagcgtgtg tgccattggc ggagattatt
      361 ccggatgcca gcgtaacgtt taatgtgaat aaactccgtc tggaaatttc agtaccgcaa
      421 atcgccatca aaagtaatgc ccgtggttat gtcccccctg aacgttggga tgaagggatc
      481 aacgcgctat tactgggata ttcgtttagc ggggctaaca gtattcatag cagcgcagac
      541 agtgattctg gcgacagcta ttttctgaat ttaaacagtg gcgttaattt aggcccatgg
      601 agattgcgca acaattcaac atggagtcgc agtagtggcc aaaccgcaga atggaagaat
      661 ctcagcagct atttgcagcg ggcggttatt ccactaaaag gcgaactgac cgtaggtgat
      721 gattatactg caggcgattt tttcgatagt gtcagctttc gtggtgtgca gctggcgtca
      781 gatgacaaca tgctgccaga cagcctgaaa gggtttgcgc ctgtggtgcg tggtatcgcc
      841 aaaagcaatg cccagataac gattaagcaa aatggttaca ccatttacca aacttatgta
      901 tcgcctggtg cttttgaaat tagtgatctc tattccacgt cgtcgagcgg tgatttgtta
      961 gttgaaatca aagaagcgga cggcagcgtc aatagctaca gcgtaccgtt ttccagcgtg
     1021 ccattactcc agcgtcaggg gcgaatcaaa tacgcggtga cactggcgaa atacagaacc
     1081 aatagtaatg aacagcagga gagcaaattt gcccaggcca cgttgcagtg gggcggaccg
     1141 tggggaacga catggtatgg tggtggacaa tatgctgaat attaccgtgc cgccatgttt
     1201 ggtctgggat ttaaccttgg cgatttcgga gcaatttcgt tcgatgcgac ccaggcgaag
     1261 agtacgctgg cagaccaaag cgaacataaa ggtcagtcat atcgttttct gtatgccaaa
     1321 acgctcaacc aattgggcac taattttcaa ttgatgggct atcgctattc gacgtcgggt
     1381 ttctacaccc tttccgacac catgtataaa catatggatg gctacgaatt taatgacggt
     1441 gatgatgaag atacgccgat gtggtcgcgt tattacaatt tgttttacac caaacgtggc
     1501 aaactgcagg tcaatatctc ccagcaatta ggcgagtacg gttcgtttta tttaagtggt
     1561 agccagcaaa cttactggca taccgatcaa caggatcggc tattacagtt tggctacaac
     1621 acgcaaatta aagatctctc gctgggggtt tcctggaact acagtaagtc ccgtggtcaa
     1681 cctgatgctg atcaggtgtt tgcactaaat ttttccctgc cgctcaatct gttgctcccc
     1741 agaagtaatg atagctatac caggaaaaaa aattacgcct ggatgacctc taacaccagt
     1801 atcgataacg aagggcacat tacacaaaac ctgggtttaa cggagacact actcgatgac
     1861 ggtaacctga gctacagcgt gcaacaggga tataacagcg aggggaaaac ggctaatggt
     1921 agcgccagca tggactacaa aggggcgttt gcagatgccc gagtgggcta caactacagc
     1981 gataacggca gtcaacaaca actgaactac gctctttcag gcagtttagt tgcccattca
     2041 cagggtatta ccctggggca atcgctgggg gaaactaacg tgctgattgc agcaccaggc
     2101 gcagagaata ctcgtgtggc gaacagcacc gggctgaaaa ctgactggcg cggatatacc
     2161 gttgtcccct atgccacttc ttatcgggaa aatcgaatcg cacttgatgc ggcatcgtta
     2221 aaacgtaacg tggatcttga aaatgcagta gtcaacgtgg ttcccaccaa aggggcgttg
     2281 gttctggcgg agttcaatgc ccatgcggga gcaagggtat taatgaaaac atcaaagcag
     2341 ggtataccgc tgcgttttgg cgcgatagcg acgctggacg gcatacagac taatagcggc
     2401 attattgatg atgatggctc gctctatatg tccggtttgc ccgctcaggg agcgataact
     2461 gtacgctggg gcgaagctcc cgatcaaatt tgtcatatca gttaccagct taccgaacaa
     2521 caaattaact ctgcgattac gcgaatggat gccatatgca gataatcttt ggagaaaaat
     2581 gcgtgttatt actacgacta ttttttgccg ccgtcttaat gctatggtgc gctcaaactg
     2641 ctgcttatat cgggcagtgt cataccactc aggggaatcc gtatattggc gtcaattttg
     2701 gcgttaaaac tctggaggaa gaagaaaata cggctggagt agttaaagac aaattttatc
     2761 agtggaacga atcgaatgat tattatgttt cctgtgattg cgataaagac aatgtcagaa
     2821 gtggccgatg ggcattcgcc gcggattcac cgttagtcta tttaggcgac aactggtaca
     2881 aaattaatga ctatcttgcc gccaaagttt tattgcaggt taaaggcagt tctcctactg
     2941 cggttccttt cgaaaacgtg ggcacagggg cagatacacg atggcatatt tgcgatcccg
     3001 gcggtcaacg tttaggtggc cagggggcta gcggtaatag cggtagcttt tccctgaaaa
     3061 tattgcagcc gttcgttggt tcggtcgtca ttcctcctat ggcgctggcg cgattatttg
     3121 aatgctacaa catacccgca ggtgattcct gcacgactac tggtacaccg gttttagtgt
     3181 attacctgtc tggtacgatc aattcacttg gctcatgttc cgtcaatgcc ggagaaacaa
     3241 tcgaggttga tctgggcgac gtatttgcgg ctaactttcg tgttgtaggg cataagcctc
     3301 ttgaggccag aacggcagaa cttgcaattc cggtcaggtg taacacggga aacgcagggt
     3361 tagttaacgt caatctgagt ctgacggcaa cctcagaccc cagctatccc caggcgatta
     3421 agacgtcacg tcctggcgtg ggcgtggtgg tgaccgatag ccagaacaac attatttccc
     3481 ctgctggtgg gacactaccg ctctctattc ctgatgatgc agacagtatc gcgcgaatga
     3541 atgtctatcc agtcagcacg acaggcgtac caccagaaac cgggcgattt gaagccacgg
     3601 caacggtgag aataaatttt gattaacgcg tgaacgtatg aagaaaaaaa cgatatatca
     3661 gtgcgttatt ttgttcttta gccttcttaa catccatgtc gggatggctg ggcctgaaca
     3721 agtcagtatg catatttatg ggaatgtggt cgatcagggc tgtgatgtcg ccaccaaaag
     3781 tgcattacaa aatattcata ttggtgattt taatatcagt gattttcagg ccgcgaatac
     3841 cgtaagcact gctgctgatt tgaatattga tatcaccggt tgtgccgctg gtattactgg
     3901 cgcggacgtc ctttttagcg gcgaggctga cacccttgcg ccgacactgc tcaaactaac
     3961 tgacacaggc ggaagcggtg gtatggcaac ggggattgcc gtgcaaattc ttgatgcgca
     4021 aagtcagcaa gaaatcccgc tcaatcaggt ccagcctctt acgcccttaa aagccgggga
     4081 taacacactc aaatatcaac ttcgttataa gtccacaaag gcgggagcaa cgggcggtaa
     4141 tgcgacggcg gttctctatt ttgatctggt ttaccagtga aggagtgatg ttgaaacgga
     4201 taatctggat tctgttctta ctgggattaa cggggggctg tgagctattt gcccatgatg
     4261 gcacggtcaa cattagcgga tcgtttcgcc gtaatacatg cgtgctggca caggatagca
     4321 agcaaatcaa tgtgcagtta ggcgatgtct cgctgacacg tttttttcat gggaattatg
     4381 gcccggagaa atctttcatc attaatttgc aggattgcgg aacagatgtc agcacggttg
     4441 acgtcacctt ttcaggaacc cccgacggag tgcagagcga aatgctgtcc attgaaagtg
     4501 gtacggatgc agccagtggg ctggcaattg cgattctgga tgatgcgaag atattgattc
     4561 cgcttaacca ggcgagtaaa gactacagcc tacatagcgg taaggtgcca ttaacttttt
     4621 atgcgcaatt gcgacctgtc aatagcgatg tgcagtcggg taaagtgaat gccagcgcga
     4681 catttgtact tcattatgac taatacgtgg aacagattag cgctcttgat tttcgccgtt
     4741 ttatcgctgc tggtggcggg tgaattgcag gcaggagtgg tggtcggcgg aacgcgattt
     4801 atctttccgg cagacagaga atcgatatct attttactga ctaataccag tcaggaatcc
     4861 tggcttatta acagcaaaat caatcgccca acgcgttggg ccggggggga agcgtcgaca
     4921 gtgccagcac tattactggc cgctccgcca cttattctcc tgaagcccgg tactacaggc
     4981 acgttgcgct tgctgagaac ggaaagcgac atcttgcctg tggatcgcga aacgctattt
     5041 gagttaagca ttgccagcgt gccatccggc aaagttgaaa atcagagcgt aaaagtggcg
     5101 atgcgctcgg tatttaaact gttctggcga cccgaaggtt tgccgggcga cccgctggaa
     5161 gcttatcaac aattacgctg gacacggaat tcgcagggtg tacaactcac taacccaacg
     5221 ccttactaca ttaacctgat tcaggtgagc gtaaatggta aagcgttaag taatgcggga
     5281 gtcgtgccgc ctaaaagcca gcgtcagaca aactggtgtc aggtaatagc accgtgtcat
     5341 gtcgcctggc ggacgataaa tgattacggc gggttgagtg caaagaagga gcaaaatctg
     5401 ccctgaaaca ggttctgaaa acgtttgcgt tttttttgcc gcaggtcaat tcccttttgg
     5461 tccgaactcg cacataatac gcccccggtt tgcacaccgg gaatccagga gagttcatgt
     5521 actacccctt cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct catgagttta
     5581 cttttcagca attacgccgc attaccggaa caccgtttga agcactggtg cggcagaaag
     5641 tgcctgcgaa acctgttaac tgcatgggcc tgacgtttaa aaatccgctt ggtctggcag
     5701 ccggtcttga taaagacggg gagtgcattg atgcgttagg cgcgatggga tttggatcga
     5761 tcgagatcgg taccgtcacg ccacgtccac agcctggtaa tgacaagccg cgtctctttc
     5821 gtctggtaga tgccgaaggt ttgatcaacc gtatgggctt taataatctt ggcgttgata
     5881 acctcgtaga gaacgtaaaa aaggcccatt atgacggcgt cttgggtatt aatatcggca
     5941 aaaataaaga tacgccagtg gagcagggca aagatgacta tctgatttgt atggaaaaaa
     6001 tctatgccta tgcgggatat atcgccatca atatttcatc gccgaatacc ccaggattac
     6061 gcacgctgca atatggtgaa gcgctggatg atctcttaac cgcgattaaa aataagcaaa
     6121 atgatttgca agcgatgcac cataaatatg tgccgatcgc agtgaagatc gcgccggatc
     6181 tttctgaaga cgaattgatc caggttgccg atagtttagt tcgccataat attgatggcg
     6241 ttattgcaac caataccact ctcgatcgtt ctctggttca gggaatgaaa aattgcgatc
     6301 aaaccggtgg cttaagtggt cgtccgcttc agttaaaaag cacagaaatt attcgccgct
     6361 tgtcactaga attaaacggt cgcttaccga tcatcggtgt tggcggcatc gactcggtta
     6421 tcgctgcgcg tgaaaagatt gctgcgggtg cctcactggt gcaaatttat tctggtttta
     6481 tttttaaagg tccgccgctg attaaagaaa tcgttaccca tatctaatta tttcttcgaa
     6541 ttcctcttta taagataatc agggctttat tttcagccct ggttgtttta tattcatcgt
     6601 tgttgcttat ttagacattt tgtactttta ttgttgaggt tattaagcga agcgacaatg
     6661 gattgtgttg ctgcggtttt atagggtagg ggagaggcag atgcgaatta aaccagacga
     6721 taactggcgt tggtattacg atgaagagca cgatcgaatg atgctcgatt tagccaatgg
     6781 tatgctattt cgctcacgtt ttgcgcgcaa aatgttgact ccagatgcct tttctcccgc
     6841 aggcttttgc gttgatgatg ccgcgctcta tttctctttt gaagaaaagt gccgcgattt
     6901 taatttatct aaagaccaaa aagccgaact ggtgctgaat gcactggtag caattcgtta
     6961 tctcaaaccg caaatgccga aaagctggca ttttgtctcc catggtgaaa tgtgggtgcc
     7021 catgccgggc gatgcggcct gcgtttggtt gagcgatact cacgagcagg tcaatttgct
     7081 ggtcgttgaa tctggcgaaa atgccgcgct atgcctgctg gcacaaccct gcgttgttat
     7141 tgcgggtcgc gcgatgcagt tgggcgacgc cattaaaatc atgaacgaca ggctgaaacc
     7201 gcaggtgaat gttgacagct tcagcctcga acaggcagtc taacgcgtca gcttaagggc
     7261 agtcttcggt acacagctac agcaaagaat ggtgccatca tcgcccattg ctgatttttt
     7321 cagcggcgtg acttcgcctt ctaaaagctg aacacggcaa cttccacaaa tgcccgcgcg
     7381 gcaagaataa gggatacgaa ttccctgatt ttctaattgc tccagcaaca cttgttggtt
     7441 atttccacga aatgcctgtc cctgccagtc aatatctaca tttgcgtccg gttgttgcgt
     7501 gatgttggcg gtatcatcag cggcagctgc gccgtaaatt tttgccggag ccgttgccag
     7561 aatttccacc tcatcgccaa cgcgaatcac gccgctatta cgggcaatta aattctgacc
     7621 aaaatcgaca tcgccgttat cctgggcagt gcggaaagat tgcaatgttt ttaatggttc
     7681 gcctgccgga tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac agcggctaca
     7741 aggtttaacc acatcaaaca ccacatcacc aatgcgaatc actttccagc tatcttcttc
     7801 ccaggctgac gcgccggaaa ccaccagatt ggggcggaat tgctccattt ttacactggc
     7861 tggacaacgt tgttggagat cacgtaacga ggcttcgtta gcaagaaggt aaggatagcc
     7921 atcagcaaat gacagaggta cagtgttgtg gcgtttcacg cgccgggtca tttgtggccc
     7981 cacccagcgt aattgcactt cgcgggagaa aaatccactt agccatttgt tgatcgcgtc
     8041 tggcgcaatt cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt cttgtgtggc
     8101 gaaatcagca aaacgaacat atgcgctact gccatctggt gcggtgagat gcaagccatc
     8161 atgcacaggt gaaggagtaa accgtaccat ctggggaaac tggcgagcgg taataaacgt
     8221 accgtcaggt tccgtgatca taaagatgcg atcgaaggcc agaccactga catcagccag
     8281 agcatgtgta agaccaatgc cgcgcatcga tttaacagga tgaataaaaa gccgggttaa
     8341 tgtcgccacg gtgcggtcct caaatgaaaa taagccctca actttatgac atgacgcgct
     8401 tattagctat aatgcgcaac aattttctta gcgcctgtcc cgttaggcta ttttatttgt
     8461 cagtttggcc ctgggttgtg ctcgaaatcc tcacgtactt gtgtacgctc cggtttctcc
     8521 gcgctctcca tgtccagact gactgcaaca attacgccta ctgcggtagg ctcttagagt
     8581 aaaagtgacg atatgaattc tctgtttgcc agtacggccc gtgggctgga agagctgtta
     8641 aaaactgaac tggaaaatct gggggccgtt gaatgccagg tggttcaggg tggggtccat
     8701 ttcaagggcg acacacggct tgtttaccag agcctgatgt ggagccgctt ggcctcgcgt
     8761 attatgttgc cgctgggcga gtgtaaggtt tacagcgatt tagacctcta tctcggtgtt
     8821 caggcgatca actggacaga gatgtttaat cctggcgcga ccttcgctgt ccacttcagt
     8881 ggtttgaatg acaccatccg caacagtcag tacggcgcga tgaaagtgaa agacgcgatc
     8941 gtcgatgctt tcacgcggaa aaatctgccg cgtccaaatg ttgatcgcga tgcgccggat
     9001 atccgcgtta acgtctggct gcataaagaa accgccagta tcgctcttga tctgagtggt
     9061 gatggtttac atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat caaagaaacc
     9121 ctggcagccg ctattgtgat gcgatccggc tggcagtcag gaacaccgct gctcgatccg
     9181 atgtgtggtt ccggtacgtt gctgattgaa gcggcgatgc tggcgaccga tcgcgcacca
     9241 ggcttgcacc gtgggcgttg gggctttagc ggctgggcgc agcatgatga agctatctgg
     9301 caggaagtga aagcggaagc gcaaactcgc gcccgtaaag gcctggctga gtatagctct
     9361 catttttacg gttcggacag cgacgcacgg gtgattcaac gtgcgcgcac taacgcccgt
     9421 cttgcgggga ttggtgaact gatcaccttt gaggtgaaag atgtcgcgca actgaccaat
     9481 ccgctgccga aagggccgta cggtacagtg ttgagcaacc cgccatacgg tgaacgtctg
     9541 gacagcgaac cggcgctgat tgcgctgcat agtctgctgg gccgaatcat gaaaaaccag
     9601 ttcggtggct ggaatctctc tttgtttagt gcctcgccgg atctgctaag ctgcctgcag
     9661 ctgcgtgcag acaaacagta caaggcgaaa aacggcccgc tggactgcgt acagaaaaat
     9721 taccacgttg ccgaaagcac accagacagt aaaccggcaa tggtagcgga agattacgcc
     9781 aaccgcctgc gtaagaacct caaaaaattc gagaagtggg cacgccagga agggattgaa
     9841 tgttaccgcc tgtatgacgc cgatctgccg gaatataacg ttgccgttga ccgttatgcc
     9901 gactgggcgg tggtgcagga gtatgcgccg ccaaaaacta ttgatgcgca caaagcgcgt
     9961 cagcgtctgt tcgatattat cgctgcaacc atttcggtac tggggattgc gccaaacaaa
    10021 ctggtgctga aaacccgtga acggcagaag ggcaaaaacc agtaccagaa actgggcgag
    10081 aagggcgagt ttcttgaagt taccgaatat aacgctcact tgtgggtgaa cctgacggat
    10141 tatctcgata ctggtctgtt cctcgatcac cgtatcgccc gccgtatgct cggtcagatg
    10201 agcaaaggta aagatttcct caacctgttt tcttataccg gcagcgccac cgtgcatgcg
    10261 ggattaggcg gtgcacgcag caccaccacc gtggatatgt cgcgtactta tctggaatgg
    10321 gcagaacgca acctgcgtct gaatggcctg accaggcgtg cgcatcgcct gattcaggcc
    10381 gattgcctgg cgtggctgcg tgaggcaaat gaacagttcg atctgatctt tatcgatccg
    10441 ccaaccttct ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg cgatcatctg
    10501 gcgctgatga aagatttgaa acgtctgctg cgtgcaggtg ggacgatcat gttctcgaac
    10561 aacaaacgtg gcttccgtat ggatctcgac ggcctggcaa aactgggact gaaagcacaa
    10621 gaaattacgc aaaaaacgct ctcccaggat ttcgcccgta accgccagat ccacaactgc
    10681 tggctgatta ccgcagcctg aaaggaatag taatgtcatt aatcagtatg catggcgcat
    10741 ggctgtcgtt cagcgacgcg ccgcttctcg ataacgcaga actgcatatc gaagataacg
    10801 aacgtgtttg tctggtgggc cgcaacggcg caggcaaatc gacgttaatg aaaatcctca
    10861 accgtgaaca agggctggat gacggtcgca ttatttatga gcaagatttg attgtagctc
    10921 gtctgcaaca ggatccgccg cgtaacgttg agggtagcgt ttatgatttc gttgccgaag
    10981 gcattgaaga acaagcggaa tatctgaaac gctatcacga tatttcgcgc ctggtgatga
    11041 acgacccgag cgagaaaaat ctcaacgaac tggcgaaggt tcaggaacag ctggatcacc
    11101 acaacctgtg gcagctggaa aaccgcatca acgaagtgct ggcgcaactg gggttagatc
    11161 ctaacgttgc gctgtcgtcg ctttccggcg gctggttgcg taaagcggca ttaggacgcg
    11221 cgctggtgag caatccgcgc gtattgctgc tggacgaacc gactaaccat ctggatatcg
    11281 aaaccatcga ctggctggag gggttcctta aaaccttcaa tgggacgatt attttcatct
    11341 cccacgaccg ttcgtttatc cgcaatatgg cgacgcgcat tgttgatctc gatcgcggca
    11401 agctggtgac ctatccaggg aattacgacc agtacctgca ggaaaaagaa gaagccctgc
    11461 gcgtggaaga actacaaaat gccgagttcg atcgcaaact ggcgcaggaa gaagtgtgga
    11521 ttcgctaggg gatcaaagca cgccgtaccc gtaatgaagg ccgcgtacgc gccctgaaag
    11581 cgatgcgtcg cgaacgtggt gaacgtcgcg aagtgatggg taccgcaaag atgcaggtgg
    11641 aagaggccag ccgctccggt aagatcgttt tcgaaatgga agacgtttgc taccaggtta
    11701 acggtaagca actggtgaaa gatttttctg cccaggttct acgtggcgac aaaattgccc
    11761 tgattggtcc gaatgggtgc ggcaaaacca cgctgctgaa actgatgctc ggtcagcttc
    11821 aagcggacag cgggcgtatt cacgttggca ccaaactgga agtggcttat ttcgatcagc
    11881 accgcgcgga actggatccc gataaaacgg tgatggataa ccttgccgaa ggtaagcaag
    11941 aggtgatggt taatggcaag ccacgccacg tattgggcta tttgcaggac tttctgttcc
    12001 atccgaaacg ggcgatgacg ccagtacgtg cgctttctgg cggtgagcgg aaccgcttgc
    12061 tgctggcgcg tttgttcctc aaaccaagca acttattgat tcttgacgaa ccgaccaacg
    12121 atcttgatgt cgaaacgctg gaactgctgg aagaactgat cgacagctat cagggcacgg
    12181 tattgctggt tagccacgat cgtcagtttg tcgataacac cgttacagaa tgctggatct
    12241 tcgaaggcgg cggtaaaatt ggtcgttatg ttggcggtta tcatgatgcc cgtggtcagc
    12301 aagaacaata tgtggcactc aaacagcctg cggtgaaaaa aagcgaagaa gccgccgcgc
    12361 caaaagcaga aactgtaaaa cgcagcagta gcaaactaag ctataaattg cagcgcgaac
    12421 tggagcagct accgcaattg ctcgaagatc tggaggcgaa gctggaagcc ctacagacgc
    12481 aagtggcgga tgcttccttc ttcagtcagc cgcatgagca gacgcaaaaa gtgcttgctg
    12541 atatggctgc tgcagagcag gagctggagc aagcctttga acgctgggag tatcttgaag
    12601 cgttaaaaaa tggtggctga tcgcaaaata gtcgatttaa acagagacag ctgacaacgg
    12661 taaaattgcc tgatgaactg ggtttatcag gcatgaaaac caacactttg ttagtcatct
    12721 gtaaaatagc gcatcattaa ggagtaccaa tgtgcgaaca tcatcatgcc gcgaagcaca
    12781 tcctgtgctc gcagtgtgac atgctggtgg cgttaccgcg ccttgagcat ggtcagaaag
    12841 cggcatgtcc ccggtgtggc acaacgttaa ccgtggcgtg ggatgcccct cggcagcgtc
    12901 ccaccgccta tgcgttggct gcactgttca tgctgttgct gtccaacttg tttccttttg
    12961 tgaatatgaa cgttgcagga gttaccagtg aaattacatt actggaaatt cccggcgtgc
    13021 ttttttctga ggactacgcc agcctcggca cctttttcct gttgtttgtg caactggttc
    13081 ccgcgttttg tctgatgacc attctgttac tggtgaatcg cgcggaatta ccggtccgtt
    13141 taaaagagca actggcacgg gtgctttttc aactcaaaac ctggggaatg gcggagattt
    13201 tcctcgcggg tgtgctggtc agtttcgtta aactgatggc ttacggcagc attgggatag
    13261 gcagcagctt tctcccctgg tgtttatttt gtgtcctgca actgcgcgct tttcagtgcg
    13321 ttgatcgtcg ctggttatgg gacgacatcg ccccgatgcc agaactgcgc cagccgctaa
    13381 aaccaggcgt cacggggata cgtcaggggc tgcgttcgtg ctcctgttgt acggcaatcc
    13441 ttcctgctga tgaacccgtg tgcccgcgtt gtagtaccaa agggtacgtt cggcgtagaa
    13501 acagcctgca gtggacactc gcgctgcttg taacgtccat catgctgtat cttccggcta
    13561 atattttgcc catcatggtg acggatttat taggctcgaa gatgccgtcg acgattctcg
    13621 ctggggtcat tctgttatgg agcgaaggct cttatcccgt cgctgcggtg atctttctgg
    13681 ccagtattat ggtgccaacg ttaaagatga tcgccatcgc gtggctgtgt tgggatgcca
    13741 aagggcatgg caagcgcgac agtgaaagaa tgcatttgat ttatgaagtt gttgagtttg
    13801 taggccgctg gtcgatgatt gacgttttcg ttatcgcggt gctctcggcg ctggtgcgta
    13861 tgggaggttt aatgagtatt tatccggcaa tgggtgcatt aatgtttgct ttagtcgtca
    13921 taatgacaat gttttctgct atgacgtttg acccgcgttt gtcgtgggat cgtcaacctg
    13981 aatcagagca tgaggagtcc tgacagtatg gaatctaata atggggaagc caaaatccag
    14041 aaagtgaaga actggtctcc cgtgtggata tttcctatcg tcacggcgct cattggggcc
    14101 tgggttcttt tttatcatta cagccatcag ggaccggaag tgaccctgat caccgcgaat
    14161 gcggaaggaa ttgaaggtgg caaaaccacc attaaaagcc gtagcgttga cgtcggcgtg
    14221 gttgaaagcg ccacactggc tgatgatttg acgcacgttg aaatcaaagc gcggctgaat
    14281 tccggtatgg aaaaattgct gcataaagac accgtctttt gggtggtgaa accgcagatt
    14341 ggtcgcgaag ggattagcgg cctgggaacg ctgctgtctg gagtttatat cgaactgcag
    14401 ccaggcgcga aaggcagcaa aatggataaa tacgatttgc tggactcacc accgttggcc
    14461 ccgcctgatg cgaaaggtat ccgtgtgatt ctcgatagca aaaaagccgg gcagctctcg
    14521 ccaggagatc cggtgctgtt ccgtggctat cgggtaggtt cggttgaaac cagcaccttc
    14581 gatacgcaaa aacgcaatat cagttatcaa ctgttcatca atgcacctta tgaccgactg
    14641 gtgaccagca atgttcgctt ctggaaagat agtggcattg cggttgatct gacgtcagcg
    14701 gggatgcgtg tggagatggg ctcattgaca acgctgctta gtggcggtgt tagctttgat
    14761 gtgccggaag gtctggattt agggcagcca gtggcaccga aaacagcttt cgttttgtat
    14821 gatgatcaga agagcattca ggattcgttg tacaccgatc acattgatta tctgatgttc
    14881 tttaaagatt cggtacgcgg tctgcaaccg ggagctccgg tagaattccg gggtattcgc
    14941 ctgggtaccg taagcaaagt gccattcttt gcgccgaata tgcgtcagac atttaacgat
    15001 gattaccgta ttccggtact gattcgtatc gagccagagc ggctgaaaat gcagcttggc
    15061 gaaaatgcgg atgttgttga gcaccttggc gaattgttga aacgtggttt acgcggatcg
    15121 ctgaaaaccg gaaacctggt cactggtgca ctgtatgttg atctcgattt ctatccaaat
    15181 acgcctgcaa taaccggtat tcgtgaattt aatggttatc agattatccc gactgttagc
    15241 ggcggcctgg cgcaaatcca gcaacgactg atggaagcgt tggataagat caacaaactg
    15301 ccattgaatc cgatgattga acaggcaacc agtacgcttt ctgaaagtca gcgcacaatg
    15361 aaaaacctgc aaacgacgct ggatagcatg aacaagatcc tcgccagcca gtcgatgcag
    15421 cagttgccga cggatatgca gtcaacgttg cgtgaattga atcgcagcat gcagggcttc
    15481 cagcctggct ccgcagccta caacaagatg gtggcggata tgcagcgcct tgatcaggtg
    15541 ttgcgagaac tgcaaccggt gctgaaaacg ctcaatgaga agagtaacgc gctggtattt
    15601 gaagcgaagg acaaaaaaga tccagagccg aagagggcga aacaatgaaa aagtggctag
    15661 tgacgattgc agcactgtgg ctggccggat gcagctccgg cgaaattaat aaaaactatt
    15721 accagttacc tgtggtgcag agcggtacac aaagtaccgc cagccagggc aatcgtctgt
    15781 tatgggtaga gcaggtcact gttcctgact atctggcggg gaatggtgtg gtttatcaaa
    15841 ccagtgatgt gaagtatgtg attgccaaca acaacttgtg ggccagcccg ttggatcaac
    15901 agttgcgcaa caccctggtt gccaacctga gtacgcaact gcccggctgg gtggttgcct
    15961 cccagcctct gggaagcgcc caggacacgc tcaatgttac cgtaacggag tttaacggtc
    16021 gctatgatgg caaggtcatt gtcagtggtg agtggctgtt gaaccaccag ggacaactga
    16081 tcaaacgtcc gttccgtctg gaaggagtgc aaactcagga tggttacgat gagatggtta
    16141 aagtgctggc cggtgtctgg agtcaggaag ccgcttctat tgcacaagag ataaagcgtc
    16201 taccttaatt ataaagattt gtaaatataa ccgtctccgg tatgttgcct gaggcggttt
    16261 ttttgtttct aacgtgcgga aaaatttgtt cctcttcaca ttttttgtac aaccgacatg
    16321 cccgtgtagc tcacaaatat gacagtggcg tgaattttgc gcattgacgg cagttatgat
    16381 tcgcggtatt gcttaactgt gattgcacat ttagtaatca ctgttttctt ttccaccaga
    16441 aaccagtatg agggaaacga ggcatgaaga gacaaaaacg agatcgcctg gaacgggcac
    16501 atcaacgtgg ttatcaggcc ggcatcgccg gacgctcaaa agaaatgtgt ccctatcaga
    16561 cgctgaatca aaggtcacaa tggctgggag gctggcgaga agccatggcg gacagggtag
    16621 taatggcctg attctgtctc tttaaaaaga aacctccgca ttgcggaggt ttcgcctttt
    16681 gatactctgt ctgatgataa tcagaaggca gacgtatcct ggaacagacc gactttcagg
    16741 tcgctggcgg tatagatcag acgacaatca accagcactt cgccatctgc cagacccata
    16801 atcagacgac gattaacaat gcgtttaaag tgaatacggt aggtcacttt tttcgctgtc
    16861 ggcagtacct gaccagtgaa tttcacttcg ccaacgccca acgcgcggcc tttaccttcg
    16921 ccgcccagcc agccgaggta gaaccctacc agctgccaca ttgcgtccag gcccaggcat
    16981 cccggcataa ccggatcgcc aatgaagtgg catccgaaga accacagatc cggattgata
    17041 tccagttctg cttcaacata ccctttgtcg aagttaccac ccgtttcggt cattttgacc
    17101 acacggtcca tcatcagcat gttcggtgct ggcaattgcg ggcctttagc gccaaacagt
    17161 tcaccgtgac cagaggcaag aaggtcttct tttgtatagg attcgcgttt atctaccatg
    17221 ttctctgtaa gccttatttt attgaagcac gcaggatagc taacacgtgt acgctgaaca
    17281 agtccgatca gttcggaata aaccagttca gccaacgtaa tggccatgga aaacggtgac
    17341 gtccttcctg ttgcgatgct tgcgcgatac gttcctggat ggtttgcatc agcgtcgttt
    17401 ggccttcgcc atcccacacc agatttaata ataacggcag tgcgtcagtc acatcgtcta
    17461 ctgcccagat ggtgaatttg ccttcttcta ccgctttcac cagttcactg tgaagactta
    17521 aatggcgaac gttagcagtg gggataatga caccttgttt ccccgttaac tcacgttgct
    17581 ggcaaatagc aaagaagcct tcgattttct catttaaacc accgaccggc tggacgcggc
    17641 cgaactgatc gactgaacct gtgatagcga tactctgatt cactggcaca tcagcgaggg
    17701 cgcttatcag ggcgcagagt tcagccatcg aggcactatc gccatccact tcactgtatg
    17761 actgctcaaa tgtcagcgat gctgagaagg ggatctgttg ctcaagctgt agttccgaca
    17821 tcaggaacgc ctgcatgatc atcatccctt tcgcatggat attgccgcca agctccgctt
    17881 tgcgttcgat gtcggtgaat tcaccatcgc caatatgcac aacgcagcta atgcgagaag
    17941 gttcgccaaa agcgcgtgga tgacccggaa attcaatgac cgaaagggcg ttaatttgcc
    18001 cgatgcgttc gccttcggtt tcaatcagga tttgctcctg aaggatctca tcctgcatac
    18061 gttcagcgag gaaaccttcg cgccattcac gctgctgcag cattaagttt agctgctcgc
    18121 cggagaaggt gtcgccatca cacagggagg cgacctcttt acactggcgg aggatccact
    18181 gcgggctaag cggaagtgtt tcttgttcac cggtgtagcg tgctgcttcg cggataagta
    18241 ccggccaagc atccgctccc ggggcaggta agtgattatg tctggcggta aatgtcaccc
    18301 agcgacacca ctggcttact gactccgcat cgacaatctg cagagtatct tcaaattcgc
    18361 tataaatagc ctgctctgaa agctctggct ccatctcctg gaaatcagcc aatgattcgc
    18421 gttcgcctac cagaatgact ttcagcttca atggcatcga aggcacagag acggggagag
    18481 ggcgcgactc atcaaacgca acccagtcaa aacgctcgcg gttaacgata tttttcagcc
    18541 gcatccacag cagaggttgc gccagcagtg tacgcaaaga gatgatgaga ataccgccat
    18601 ttgcctgatg caccagacca ggctgcaggg taatgtcgcc attaaactgg cgcaggcagc
    18661 caaagagttg ctccgcttct acccagtcgg cagcgacaac ttgcgttaaa gtcgcaaaat
    18721 tatcatctgc actcactgcg tgacgtaagc ggatggagtg gccggaaacc tcatagtgat
    18781 cgcccaccag ttggcctgca tcgctttgta gcgtacgcgc ggcatcggca ataagattca
    18841 gatactcaga ctcttccggg gccttagcca gcataaaaga ggaggatgct cgcgtatgca
    18901 gcaactgctc cagcgcaaat tgcagtcgcg gttgagtatc actgaataaa ggatcgtttt
    18961 cgtcaatcaa atgtggctga gcaaatattt cctgatagct atcggtatca ggaaccaggt
    19021 cacgccatgc aagtttcgta atggtcaaag ttgatgtttt ttagtctgtt gtcaaagccg
    19081 cgattatacc gtaaccggca ctacagcaca cgtagaaagc accgacaata ctcttggctg
    19141 ggcgttaaag ctcacaggat ggagattctt ttcttcactg gcctaaaaag ctgatattct
    19201 gtaaagagtt acacggtaac attgagatcg ctatgaaata tcaacaactt gaaaatcttg
    19261 aaagcggttg gaaatggaag tatctggtga agaagcatcg cgaaggggag ttaattaccc
    19321 gttacataga agccagtgcc gcccaggaag ccgtcgatgt gttgctctcg ctcgaaaatg
    19381 aacccgtgct ggtaaatggc tggattgaca agcatatgaa tccggaactg gtcaatcgca
    19441 tgaagcagac cattcgggcc agacggaagc gccattttaa tgcggaacac cagcatacgc
    19501 gcaaaaaatc gatcgatctg gaatttattg tctggcaacg tctggctggt cttgcacagc
    19561 gtcgcggtaa aacgctttct gaaacgattg ttcagctgat tgaagatgcg gaaaacaaag
    19621 agaaatacgc gaataaaatg tcttctctga agcaggatct gcaggcattg ctgggtaagg
    19681 aataactgac gaaagtcagt tcaatttact aaaggcaaaa aaaaccccgc agcagcgggg
    19741 tttttctacc agacgataac ttaagcctgc ggctgagtta caacgtcttt gataccttta
    19801 acttcgatct ctacgcgacg atccggagcc aggcagtcga tcagtgcagc acgctgtttc
    19861 acgttgtcac aggtgttgcc agtaaccggg ttggattcgc ccataccacg tgcggagatc
    19921 ttgtctgccg ggataccttt ggagatcagg taatcaacaa cagactgagc acggcgctcg
    19981 gacagaccct ggttgtaagc gtcagaaccg atgcggtcag tgtaacccag aacaactacg
    20041 gaaccgtctt tcggatccag gttgctcagc tggctgtaca gctgatccag agcagcctga
    20101 ccttccggtt tcagggttgc tttgttgaag ttgaacagaa cgtcagactt cagagtgaag
    20161 tgcttggtct gtacttccgg tgccggagct ggagcaacta ccggagctgc ttcgccctga
    20221 ccgaaacggt aggaaacacc caggctcagc agaccgttgt ccggacgagt accgatggtg
    20281 tttgcgtcac cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg
    20341 atcgcgtact caacaccacc tgcgaaaacc ggagaaacgc cagtgtcgtg gtctttaaag
    20401 gatgcgccac taggtacgtt agccttggtg tctgcacgcc ataccatacc acccagacga
    20461 gtgtagatgt ccagatcgtc agtgattggg taacccagtt tagcggtcag ctgaacgccc
    20521 tgagctttgt atgcgccgtt gatgttgtcg cctttgtacg gcatacgacc taaccagtcg
    20581 taacccattt caaaaccaac atacgggtta acctggtaac caccaaaagc acctgcaccc
    20641 agttggtttt cgtgggtcgg accattgtta ggaataaaac cagtgtcatg gtactgggac
    20701 cagcccagtt tagcaccagt gtaccaggtg ttatctttcg gagcggcctg cgctacggta
    20761 gcgaaaccag ccagtgccac tgcaatcgcg atagctgtct ttttcatttt ttgcgcctcg
    20821 ttatcatcca aaatacgcca tgaatatctc caacgagata acacggttaa atccttcacc
    20881 gggggatctg ctcaatatta actctaccga tatcttcggc ttatgccgag cacccctagc
    20941 gatgtaaagt ctacaacgta gttgaaaact tacaagtgtg aactccgtca ggcatatgaa
    21001 aaaaaagtct tgtataaggt atgtttaatc tttttgtcag cgacaattta cagaaaaaaa
    21061 acgcggaaac cgcttcagac aagccttcgc aaggaaaatt agtcacgact gaaagcattg
    21121 gctgggcgac aaaaaaagtt ccaggattaa tcctaaattt acttaatgat acaaattaga
    21181 gtgaattttt agcccggaaa gttgtctcgt tgtgtgagag gatgcgctta ccggacgcat
    21241 aataaacccc atagcgttac cttcatttgc cgcatcaaca agttcagcat gctcttcttc
    21301 agtcaaatca tctgtcaacc aaccgatcac cacactgtaa ttgcccgtgc gtaaagcgcg
    21361 aaccattgac tccacagtgt ggcaagggga gagctggcta atctgcatta ctttcgttaa
    21421 gggtagccca gatgcctgaa cccattcccg actcagtttt tgttgcggtg ttaaccagag
    21481 ttgccagcgc gattgctgac cgagttgctg taacaatggc aacagtagaa gttgcgtcat
    21541 catgggctga tcttcgcgat agacaacttc actgataagc ccggctgtag tgttttccgt
    21601 agagacacgc gcaattttac ttgctgcgga tgagaacgac gaagaacgat gtgcatagcc
    21661 tgaagtgtac ataatcaatc cagcccctgt gagttactgt atggatgtgc agtacatcca
    21721 gtgacaacaa agatcaaccc tattttcgga aagagcctcg caaattttgt cgttggtgac
    21781 gggaaaacat aaattaatct tgccccttaa gaataagttg cctattttcg tagttaacgg
    21841 atccgttaat gtgaatcatt cttttatgtt atgattttaa aaggaatttt atgaaaagcc
    21901 tctcctataa gcggatctat aaatcacaag aatacctggc aacgttgggc acaattgaat
    21961 accgatcatt gtttggcagt tacagcctga ccgttgacga cacggtgttt gcgatggttt
    22021 ctgatggtga gttgtatctt cgggcttgtg agcaaagtgc acagtactgt gtaaaacatc
    22081 cgcctgtctg gctgacatat aaaaagtgtg gccgatccgt taccctcaat tactatcggg
    22141 ttgatgaaag tctatggcga aatcaactga agctggtgcg tctgtcgaaa tattctctcg
    22201 atgcagcgct gaaagagaaa agcacgcgca atacccggga aagactgaaa gatttgccca
    22261 atatgtcttt tcatctggaa gcgattctcg gggaggtggg gattaaggat gtacgggcgt
    22321 tacgtatact tggggcaaaa atgtgttggt tgcgactgcg gcagcaaaac agtctggtga
    22381 cagaaaagat tctgtttatg cttgaaggtg ccattatcgg cattcatgaa gctgcgctcc
    22441 cggtggcacg ccgccaggag cttgcagaat gggctgactc tcttacgccg aaacaggagt
    22501 ttcctgcgga gcttgagtaa tctggcgttg cagacgacca atctctggca gtaatgcaat
    22561 caataaccca acttgttgta cgaccagagg ttctttgcta tctgcccgtg gttcaagttg
    22621 ctgcatccgc tgtttcaggc cagctaatgc ctgattgacg cgttcttcat cagcaggttg
    22681 atgatgtaac gcgtcatcaa catagcaaac tgcgtcatca agaaacgcca gaatttcagg
    22741 attagttaac tgctcccggt gagcaccgag ggctgagata tagctggtaa acgtatggtt
    22801 aaggcacagc aagcgaaacg cggcttcgcg aatttgcggg gtaacgttcg gttcgctgga
    22861 catatttgat accaccgacg ccagttcagc atcacggttg tgtgcatcgc ggcgggcaat
    22921 acgatacgcc agacggttat cacgcccctg atggtattgc tccagtatgg catcgagata
    22981 gcgacagttg gcctctgtgg cgcgttcgag cattcgcggc agattgcgaa acttccagtc
    23041 aggccagatg tagctcactg ccgcccacgc aatggcacaa ccaatcagcg tatcgattac
    23101 gcgaggtaac gctacttcaa aaccttcacc cagtaagtta aaacacagta gcaccaaaag
    23161 tgtgatgaac atcgttgcat gagcgtattg cacgttacgg aaggcaaaaa agagcacgcc
    23221 ggtaataacc agcagcacca gctgcccttc cagtgatggc acaaaccaca gcacaggaat
    23281 gccaatcgcg atacctacca gcgtaccaat aatccttaac ttcaggcggt ggcgcgtggc
    23341 gttatagttt ggctggcaga cgaacaaact tgtcagcaag atccaatacc cgtgatgcat
    23401 tccggttatt tgaatgatgg cgtagccgaa gcacaacacc agcgacattc ttaccgcatg
    23461 acggaagagg gcggattccg gcgtgaagtg acggctaaga cgcagccaga tatcactcaa
    23521 tccgtgcggg ctgtcatcag cgagctcatt ttcgtcatta ttacggggta gtgcctgggc
    23581 ctgttctgat tcaattgttg ccagttgggc atcaatggca cgtaaattgt tcagcaaaaa
    23641 tcccagtgtt ttgagtaaat cggcgggtgc gccgttatcg cgcatccgct ccagcgcagc
    23701 atcaatatgc gtaaaagcgc gctcaaaatg cggatcatgt tgataagact gacgcaacaa
    23761 aatacagcgt gacagttgct ggcacgcctg gccctgcatc gacatcagcc gctgaaaacg
    23821 gaacagcacg tcgctgtggc gaaaatgttc acgcaatgtt tgatactgaa tatgagaaga
    23881 gctggcacgc tcgtgaatat cctgtgcgac aaagtaataa tgcagcgtgc gacgcgttcc
    23941 ccgttgacca cgatcgccac gtaagcgggt cagcagcgag agtttcgtct gattcaatgt
    24001 cgccatcagc tgaccgttgg cgagagccaa atcgtacagc ggtgcctggc tttcatcttc
    24061 aatatcagga tcaaacatgc gcgacttgag ctcaagataa cgcgccagtt gttcatagca
    24121 acgcgccagg ttgtcctgca gcgggcggac cgggaacagc agatgaccaa taagtgtcag
    24181 gacgttgtac cagacggcac cggccagcag atacatcggc tgctgatacc agtgctcata
    24241 cagtgatgtt cccaacatag tgtaaatggc gatcagcaat gcaccgaagg caattgttgc
    24301 atagcgttga cccagaccgc cgagcaaaat gaagccgctg gtagagagcg ttaagccaat
    24361 cgcaaatagc cagggccagg gaaacagcaa ttctactgag gccgaggcga taaaaaagca
    24421 gaacagcgta atgatgaggt tacgcaaacg tcccgccagt cggtcatcga gatcggtcag
    24481 cgccgctgcc accatcccca gcgttagcgg aatcgtcagt tttacatcac ccagccacca
    24541 cggaaacgct gtggttccac aaagcgcaat aaaaatacgc gcgtaataca gccaggcgct
    24601 gttccaggta tagcgtttga gcaaaggact tagcataaag gccatattag tggattattc
    24661 aaaacgacga cgtgcattgg cttcacgcgc agcttgcgct gtttctaccg ataccacgcg
    24721 acgaccaacc ggccatagtg caatagcggc aattttaaag ttcgcaatgc cgacaggaat
    24781 gccaatgatt gaaatacatt gtgcgatgcc cgttgcaatg tgcatcaggc ataaccacca
    24841 gccaaagaaa atcagccaga aaatattcaa taccgtaccg ccagtattca gcaacacatt
    24901 tttgccagcc gggttcagtt catcgacatg aatagcttca ttgccataag gcaccagaga
    24961 cagtttagtg atctcccagc aggatcgtgt cagcggtaag gtaaaaatca gcacaatact
    25021 gaccagagtc gccaacagcc agcccagagt ggtggcaaat ccgccaagca caaagttcag
    25081 aatgttcaaa acggtacgca taaaacctcg ctttactgtg gttttcagta atgggcggca
    25141 attgtaacgt ttttttgggc tggagcacgt tttctctgac ggttacactg ataagaaata
    25201 atttcgtgtg gatctacaga gtcatggaac tgaaagcgac aacgcttgga aaacgtctgg
    25261 cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg gattaaagtc tccggcgatc
    25321 gccacgaata ccttattcct ttcaatcaat tactggcgat tcactgtaag cgcggtctgg
    25381 tatggggcga gctggaattt gtactgccgg acgaaaaagt ggtgcgtctg cacggcacag
    25441 aatggggcga gacgcagcgt ttttaccatc atcttgatgc tcactggcgg cggtggagtg
    25501 gcgagatgag cgaaattgcg tctggtgttt tacgccagca actggatttg attgccacgc
    25561 gcactggaga aaataaatgg ctgacgcgtg agcaaacctc tggcgttcag caacaaatcc
    25621 gccaggcttt gtcggcgttg ccgttgccgg ttaaccgact ggaagaattc gataactgcc
    25681 gtgaggcgtg gcgtaaatgt caggcttggt tgaaagatat tgaaagcgct cggttgcagc
    25741 ataaccaggc gtataccgaa gccatgctta ccgagtatgc ggattttttc cgccaggtcg
    25801 agtcttcacc gctgaatccg gcgcaggccc gggcagtcgt taatggcgag cattctctgt
    25861 tagtgctggc aggtgcagga agcggaaaaa cgtcggtgct ggtggcccgt gcaggctggt
    25921 tgctggcgcg tggtgaagcg tcccctgagc aaattttatt gctggcgttt ggtcgcaaag
    25981 ccgctgaaga gatggacgag cggattcgcg aacggctaca taccgaagac attaccgcac
    26041 gcacgtttca tgcgctggcg ctgcatatta ttcagcaggg cagcaaaaaa gttccgatag
    26101 tcagcaaact ggaaaatgat accgctgccc gtcatgaact ctttattgct gagtggcgca
    26161 agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg gcaatggctg acggaagaaa
    26221 tgcagtggtc agtgccagaa ggtaacttct gggatgatga aaaattacag cgtcgccttg
    26281 cctctcgcct cgatcgctgg gtaagtctga tgcggatgca cggtggtgca caggcagaaa
    26341 tgattgccag tgcacccgaa gagattcgcg atctgttcag taaacgtatc aagttgatgg
    26401 ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga aaacgctgtc gatttttcgg
    26461 gccttattca tcaggcgatt gtgattctgg agaaaggccg ctttatcagc ccgtggaagc
    26521 atattctggt tgatgaattt caggatatct cgccgcagcg cgcagcgttg ttagcggcat
    26581 tacgcaagca aaacagtcag acgacgttgt tcgccgttgg tgatgactgg caggcgattt
    26641 accgattcag cggtgcgcaa atgtcgctca ccaccgcttt ccatgaaaac tttggtgaag
    26701 gcgatcgctg tgatttagac acgacttacc gttttaacag tcgtatcggt gaggtggcaa
    26761 accggtttat tcagcagaac ccaggccagc tgaaaaagcc gctaaacagc ttaaccaatg
    26821 gagacaaaaa agccgtcacg ttattggatg agagtcaact tgacgctttg ctggataagc
    26881 tctctggtta tgccaaaccg gaagagcgca ttctgatcct ggcgcgttac catcacatga
    26941 ggcctgccag cctggaaaaa gcggcaacac gctggccgaa gttgcaaatc gactttatga
    27001 ccattcatgc cagcaaaggg caacaggcgg attacgtcat catcgttggc ttgcaggagg
    27061 ggagtgatgg ttttccggct gcggcgcggg agtcgattat ggaagaggcg ctactgccac
    27121 cggttgagga tttcccggac gctgaagaac ggcgtttaat gtacgtggcg ctgacccggg
    27181 cacgccatcg ggtatgggca ctgtttaaca aagagaatcc ctctcccttt gtggaaatac
    27241 tgaaaaatct ggatgtgccg gtggcgagaa aaccgtaaga aacaggtggc gtttgccacc
    27301 tgtgcaatat tacttcagac ggtcagcgag ataacgctga taatcgggga taagaatatc
    27361 gaccgcgtcg ttgaaatgcg gcgactggat aatgaagtct gccgttgcca cgttggtggc
    27421 gaccggaatg ttccataccg tcgccagacg cagcaaggct ttcacgtcag gatcgtgcgg
    27481 cacggcattt agtggatccc agaagaaaat caatacatca attttccctt ctgagatcaa
    27541 tgcgccaacc tgctggtcac cccccattgg gccactcaac atcgcgttga cgttcatgcc
    27601 ggtcgcgcgg gaaatcaagt taccggtagt gcctgttgca tacagtacgt gttgttccag
    27661 taacggttga tgccgttcca cccagctcat cagcatttgt ttgcagtgat cgtgtgccac
    27721 cagcgcaata tgtttccgcg caggtaaagt gcgagtcgtc agttccataa tgtacatccg
    27781 tagttaactt tcctacagat tactgtaagc acttatcgct gcaagataaa gaccgaaaaa
    27841 gcctgcgcac aggcacaaaa atctcaggaa gatggttgtt tttccgccca ttgcaggaaa
    27901 gtatttcgcg tttgtgggtc agccagttta aaccaatact tcagccgttg ttctgtgagc
    27961 acctgagact gcggtggaat agcatccagc tcggaaacac ccgaaagcag cgtactgtca
    28021 tctgccatca tcgtggcaaa ttgcggtagt gaagcgcgtt tcgcggattt gttatagcgt
    28081 tcaacctcga cctcataatc aatcgttttt gccgttgagg taatggcgag aatatccagc
    28141 tttaccggaa tcggcgtcgc atcgccatcc aacaattcaa ggcgcggcgc ggcatcaaaa
    28201 tggttagctt cccgctcatt ctccaggcga ggcaggcgaa aattgacctg gttgatgagc
    28261 tgggtattaa aactgaccac cagcggtggg gagatataca gccgttcttc actattggaa
    28321 agatgaatcg tcttctcaac gcgaaacact aactgatgcg gtccattgtc cagttcaatg
    28381 ctgtccgcac cgcgcagcag agagctggaa accttcttgc cgtcgagtac cagaagatcg
    28441 acatcggttg aaagccgcag cgtggtggca aaaacggata ccggcaaaca taatgcaatc
    28501 aaggtggtca cgatgccggt tttcataggt agctcctgtc aaaagaccgc ccgaataata
    28561 atgtgtcaaa atttttcttt aatcatactt actaacatat agacatattt cccgcttttg
    28621 ctctcattca ttcgtattag ctgcatggtt ggcatgtcgg ctttgtcgta tactttgtta
    28681 aacagtctgg aggaaataat gaaagaaacc gatattgcag gcattttaac gtccactcac
    28741 accattgcgc tggtgggggc aagcgacaaa cccgatcgcc ccagctatcg cgtgatgaaa
    28801 tatctactcg accaggggta tcacgtcatt ccggtttcgc caaaagttgc cggcaaaacg
    28861 ctgctgggcc agcaggggta tggcacgctg gcagacgttc cggaaaaggt cgatatggtc
    28921 gatgttttcc gtaattcaga agcggcgtgg ggcgtggcac aggaagctat tgccattggc
    28981 gcgaaaaccc tctggatgca actgggggtg attaatgagc aagcggcagt actggcacgg
    29041 gatgccgggt taaatgtggt gatggatcgc tgcccggcta ttgagatccc tcgcctgggt
    29101 ctggccaaat aaaaaatccc cggaaggcaa aaaccttccg gggatttgtt cagggattag
    29161 ttacgcagac gcggggcctg gagttgtttg cggatggtct gcgccagctc atccatggat
    29221 ggctgttcag gatgttcatc ctgcaattcg ctacttagct gggcttcagc caggtaggta
    29281 tgaaccggta ggccgttatc gtcctccatc accacgtgat accacggagc ggcgcgaagc
    29341 tcgtcattca ccgccagctc atcaggcgac ggttcagaga gcgaataaac cgggtcgata
    29401 tccacgacca ctccgagata acctaacagg gaatggcgga cctgctggcc gataccgaat
    29461 ttgctggcaa tcatagtcac ctcccgggaa atctttacct ttgatataag ggtaaaattc
    29521 cacatttcaa gttacatgac gcgacaggca aaccctttca gatatagccc ttccgggtag
    29581 gtagcgatca ccggatgatc ggctgcctga cggaactgct ctataaattg tacatcacga
    29641 ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata aatcgctggt catcagaccg
    29701 gaacaggaga aagtcaggag aataccgcct tcattcagca actgaatcgc cagcatgttg
    29761 atgtctttat aaccacgaca cgcgcccatc aactggcttt tattctcaac aaacttcggc
    29821 gggtccatca cgataacgtc aaatttttca ccgcgatcgc gataagtacg cagcaattta
    29881 aagacatcat cacggacaaa ctcagccttg ctcagatcca gtttgttcag ctcaacgttc
    29941 tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc tgacaacctg gctgcaaccg
    30001 cccatcagtg ccgatacggc gaaaccaccg gtataggaga aacagttcag cacacgttta
    30061 ttttcaacgt agcggcgggt agccaggcgg ctgtctcgct ggtccagata gtagcccgtt
    30121 ttgtgtccgt gctgaatatc caccagcagc ttcatgccgt gttcttcaat cggcagcagg
    30181 gcaggcggca actcgccggt gacgaggccc tgggtcagct ccatcccttc ttttttacgt
    30241 accgcaacat cgctgcgatc gtaaatcgca cattccgggt acagcgtttg cagggcacta
    30301 attaatgccg cgcgctgata ttctgcgcca gcactcagca gttgcagcac cagaaaatta
    30361 ccgaaacgat cgatagtaat acccggcagg ccatcagatt ctccggcgat taaacgatag
    30421 ctgtcgaggc catctttttg cgccagccag tcacgccatt tttgtgcttg ttgcaaacgg
    30481 cgggaaaaaa aagcaatgtc gatagactca gacgggtcaa acgtccagac gcgcgcccgg
    30541 atttgcgaag ctggcgaata agcgccgcgt gctaaccatt ttccctgatg atcaacaata
    30601 tcgatggttt caccgaggct ggctttacct tccatgcggg caacggcccc ggaaaagacc
    30661 cacggatggc gacgaagtaa tgatttttcg cgccctttgg ctaacactaa acgtacactc
    30721 ataatttact tttctgtcga tgcaaagaaa tgggcgtcat tgtccggagt tttccatgga
    30781 tttgcaacgc gtccattaag gataaggaga gcagcaatgt cgaaagtctg cataattgcc
    30841 tggatttacg ggcgagttca gggcgtagga tttcgctaca ccacacagta cgaagcgaaa
    30901 agactgggct taaccgggta cgccaaaaat cttgacgatg gcagcgtgga agtggttgcc
    30961 tgcggtgaag aagggcaggt agaaaaatta atgcagtggt taaagagtgg cggcccgcgt
    31021 tctgcgcgtg tagaacgggt gctcagcgag ccgcatcatc cctcggggga attaaccgat
    31081 tttcgtatcc gttaaataca ttttaccggt tttggcaggc cggcaatttt ggtggcttgc
    31141 ttagccgggc ctttcgggaa cagtcggtat aaatagcggc tgttaccttt ttcttcacca
    31201 aatttattcg ccatcgcttt taccagcata cgaatcgccg gagacgtatt aaattccagg
    31261 tagaagtcac gtacaaaacg caccacttcc cagtgttctg gcgacagcga aatcccttcg
    31321 ttctctgcaa tcaccactgc cagcggttca ctccactggc tgctttcttt gagatagcct
    31381 tcggtatccg tttctatctc tttaccttcg aagatcagca taattattac taccttaatc
    31441 agactggcgg cagtgtaaca aaaaacaaag ccccgcataa agcgaggcta tgaaagtgtt
    31501 agcgggtgag attaatcgcg gctggcgaag cccagaatgc tcagcaggct gacgaagatg
    31561 ttgtacagcg aaacatacag gctaaccgtg gcacgaatat agttcgtttc accgccatga
    31621 atgatgttgc tggtttcaaa caagatagcg ccagaggaga tcagaatgaa gaccgcgctg
    31681 atcgccagat gcagagcagg cagttgcagg aagatattag caaccatacc aatcagtacc
    31741 accacaatac ccgccatcag cataccgccg aggaacgaca tatctttgcg ggtggtcagc
    31801 acatatgcag agcagcagaa gaacactaac gccgttccgc ccagtgccat agcgattacg
    31861 tcacccattc cggcagacag ataggtgttc agaattggtc cgaggatata acccagaaaa
    31921 ccggtaaagg cgaatgcgga gataatcccg gtcggcttat tcgccgtttt ataggtcagg
    31981 aacatcaaac catacatacc caccagcgtc agaatcagac ccggagatgg cagcatcagc
    32041 accgtactgg cagttgcggt aatcgccgaa aaggccagcg tcaggctcag cagaaaatag
    32101 gtattacgca gcaccttatg ggtgctaagc agtgatgtac ggtcatgtga agaactaaca
    32161 atacgatcca ttgagtcact ctctatgaca gatgtaatta attaagcagc ataatgataa
    32221 tgcgtaaggg cacccagaag ttttacccat ctttacgcat ttgatctgga acaggtttaa
    32281 cagcggatta tcaggtcatt aagcaaatat aacgccctga gaattccgac aggcaaaaga
    32341 aaaaggggtt agcatttagc taacccctta tcttatttgg cggaagcgca gagattcgaa
    32401 ctctggaacc ctttcgggtc gccggttttc aagaccggtg ccttcaaccg ctcggccaca
    32461 cttccggaat gacgcgcact ataaacatcc cgatgcggcg tgtaaacccc taatttgttt
    32521 gtttgcctga aaaatagcca aaagtgcatt gatagcgtga aataacagca gattgatcat
    32581 ttcatcacca tgaattcctt ctcttttact cgtttagcaa ccggctaaac atccccatcg
    32641 cccgggcaaa gaaaaatagg tccattttta tcgctaaaag ataaatccac acagtttgta
    32701 ttgttttgtg caaaagtttc actacgcttt attaacaata ctttctggcg acgtgtgcca
    32761 gtgcagaagg atgagctttc gttttcagca tctcacgtga agcgatggtt tgccttgcta
    32821 cagggacgtc gcttgccgac cataagcgcc cggtgtcctg ccggtgttgc taggagaaga
    32881 gacgtgcgat atgaataacg aggaaacatt ttaccaggcc atgcggcgtc agggcgttac
    32941 ccggcgcagc tttctcaaat attgtagtct ggctgccacg tcgctgggat taggcgcggg
    33001 aatggcacca aagattgcct gggcgctgga gaacaaaccg cgcattccgg tggtatggat
    33061 ccacggtctg gaatgtacct gctgtaccga atcttttatc cgctccgctc acccactggc
    33121 gaaggacgtc atcctttccc tgatttccct cgattacgac gataccctga tggctgccgc
    33181 cggaacccag gcggaagaag tctttgaaga catcatcacg caatataatg gcaaatatat
    33241 cctcgcagta gaaggtaatc cgccgctggg cgagcagggg atgttctgta tcagcagcgg
    33301 tcgaccgttt attgagaaac tcaaacgtgc cgctgccgga gccagcgcga ttatcgcctg
    33361 gggaacctgc gcgtcctggg gctgcgtgca ggccgcgcga cctaatccga cacaggcaac
    33421 gcctatcgac aaagtcatca ccgacaaacc cattatcaaa gtacctggct gcccgccgat
    33481 cccggatgtg atgagcgcca tcattactta catggtgacc tttgatcgct tgccagatgt
    33541 cgacagaatg ggtcgtccac tgatgtttta tggtcagcgt atccacgata aatgctatcg
    33601 ccgcgctcac ttcgacgccg gagagttcgt ccagagttgg gatgatgacg ctgcccgcaa
    33661 aggttattgc ctgtacaaaa tgggctgcaa agggcctacc acctataacg cctgttcctc
    33721 cacacgctgg aatgatggcg tttctttccc aatccagtct ggtcacggct gcctgggctg
    33781 tgcggaaaat ggtttctggg atcgcggttc gttctacagc cgcgtggtcg atattccgca
    33841 aatgggtact cattccaccg ccgataccgt cggtttaacc gcgcttggcg tggtggcagc
    33901 agctgttggt gtgcacgcag ttgccagcgc cgttgaccaa cgcagacgtc ataaccagca
    33961 acctacagaa accgaacatc agccaggcaa tgaggataaa caggcatgag cactcagtac
    34021 gaaactcagg gatacaccat caataatgcc ggacgccgcc tggtggtcga cccgattacg
    34081 cgcatcgaag gccacatgcg ctgcgaagtg aatattaacg atcagaatgt gatcaccaat
    34141 gccgtctcct gcggcaccat gtttcgcggg ctggagatca tcctgcaagg gcgcgacccg
    34201 cgcgatgcgt gggcgttcgt tgaacgtatc tgcggcgtct gtactggcgt acacgccctg
    34261 gcttcggttt acgccatcga agatgccatc ggtattaaag tgccggacaa cgccaatatc
    34321 atccgcaaca ttatgctggc aacgctctgg tgccacgatc atctggtgca cttctatcag
    34381 cttgccggga tggactggat cgacgtgtta gatgcgctga aagccgaccc gcgaaaaacc
    34441 tccgaactgg cgcaaagtct ctcctcttgg ccgaaatcat cccctggcta tttcttcgac
    34501 gtacaaaatc gcctgaagaa attcgttgaa ggcgggcagt tggggatctt ccgcaatggc
    34561 tactgggggc acccgcagta caaactgccg ccagaagcca acctgatggg ctttgcccac
    34621 tatctcgaag ctctcgattt ccaacgtgaa attgtcaaaa ttcacgcggt ctttggcggt
    34681 aaaaacccgc atccaaactg gattgtcggc gggatgcctt gcgccattaa cattgacgaa
    34741 agcggcgcgg tcggggcggt caatatggaa cgcctgaacc tggtgcagtc gattatcacc
    34801 cgcacggcag acttcattaa caacgtgatg atccccgacg ccttagtcat cgttcagttc
    34861 aacaaaccgt ggagcgaaat cggcacgggt ctttctgata aatgtgtcct cagctacggc
    34921 gcattcccgg atattgccaa cgactttggt gagaaaagtc tgctgatgcc tggcggcgcg
    34981 gtgattaacg gcgacttcaa caatgtgctg ccagtggatt tggttgatcc gcagcaggtg
    35041 caggagtttg tcgatcacgc ctggtatcgt tatcccaacg atcaggtcgg gcgtcatcca
    35101 ttcgatggca ttaccgaccc gtggtacaac cccggcgatg tcaaaggcag cgataccaac
    35161 attcagcagc tgaatgaaca ggaacgctac tcgtggatca aagcgccacg ctggcgcggt
    35221 aacgcgatgg aagtggggcc gctggcacgc acgttaatcg cttatcacaa aggcgatgct
    35281 gcgaccgttg agtcggtcga tcgcatgatg tcggcgttga acctgccgct ttccggtatc
    35341 cagtcaacgt tgggccgcat tttgtgccgc gcgcacgaag cgcagtgggc cgcaggtaag
    35401 ttgcagtatt tcttcgacaa gctgatgacc aacctgaaaa acggcaatct cgccactgct
    35461 tccactgaaa aatgggaacc tgcaacctgg ccgacagagt gccgtggcgt cggctttacc
    35521 gaagcgccgc gcggagcgtt aggccactgg gccgccattc gcgatggcaa gattgatctc
    35581 taccagtgcg tggtgccgac cacctggaac gccagcccgc gcgatcccaa agggcagatt
    35641 ggcgcttatg aagcggcgct gatgaacacc aaaatggcga tccccgagca accgctggag
    35701 atcctgcgta ctctgcacag ctttgatccg tgcctcgcct gttcaactca tgtgctgggc
    35761 gacgacggta gcgagctgat ctccgtgcag gtgcgttaac agcgaaggag aatcatcatg
    35821 caacagaaaa gcgacaacgt tgtcagccac tatgtctttg aagcaccagt tcgtatctgg
    35881 cactggctga cggtgttatg catggcggtg ttgatggtca ccggttactt tatcggcaag
    35941 ccgctacctt ccgtcagtgg cgaggcgacg tatctgttct atatgggtta catcaggtta
    36001 attcacttca gcgccgggat gatttttacc gtggtcttgc tgatgcggat ctactgggct
    36061 tttgttggca atcgttactc ccgcgagctg tttatcgtgc cggtatggcg taaaagctgg
    36121 tggcagggcg tgtggtatga aatccgctgg tatctgtttc tggcaaaacg tccgagtgcc
    36181 gatataggcc ataaccccat cgcccaggcg gcgatgttcg gctatttcct gatgtcggtc
    36241 tttatgatca tcaccggttt tgcgctgtac agcgaacaca gccagtacgc tatttttgcg
    36301 ccgttccgtt atgtggtgga atttttctac tggacgggtg gcaactcaat ggacattcac
    36361 agctggcatc ggctggggat gtggctgatt ggggcgtttg tgatcggtca tgtctacatg
    36421 gcgctgcgtg aagacatcat gtccgacgac acggtgatct ccaccatggt caacggctac
    36481 cgtagccaca aatttggcaa aataagtaac aaggagcgtt catgagcgag caacgcgtgg
    36541 tggtcatggg gctgggcaac ctgctgtggg ccgatgaagg cttcggcgtg cgggtggcgg
    36601 aacggctgta tgcccattac cactggcccg agtatgtgga gattgtcgat ggcggtactc
    36661 agggactgaa cttgctgggg tatgtcgaaa gcgccagcca tctgttgatt ctcgatgcca
    36721 ttgactacgg gctggaacct ggaacgctgc gaacctatgc cggagaacgc attccggcct
    36781 atctcagcgc gaagaaaatg agcctgcatc agaacagttt ctccgaagtg ctggcgctgg
    36841 cagatatccg cggacatctg ccagcacata ttgccctcgt cgggctgcaa cccgcgatgc
    36901 tcgacgacta cggcggcagc ctgagcgaac tggcacggga gcaactgccc gctgcggaac
    36961 aatcggcgct ggcgcagttg gctgcgtggg gaagtgtgcc gcaaccggct aatgaatcgc
    37021 gttgtctcaa ttatgactgt ctgtcgatgg aaaattacga aggcgttcgc ttgcgccagt
    37081 accggatgac acaggaggag cagggatgag caacgacacg ccatttgatg cgttgtggca
    37141 acgaatgctg gcgcgcggct ggacgccggt cagtgaatcc cgtcttgacg actggcttac
    37201 gcaagcgcca gacggcgtgg tgttattaag cagtgacccg aaacgcacgc cagaggtcag
    37261 cgataatccg gtaatgattg gcgaattact gcacgagttt cccgactata catggcaggt
    37321 ggcgattgct gaccttgagc agagcgaagc catcggcgat cgctttggcg cctttcgctt
    37381 tcctgccact ttagtgttta ccggcggaaa ctatcgcggc gtgctgaatg gtattcaccc
    37441 gtgggcggag ctgataaacc tgatgcgcgg gcttgtcgaa ccgcagcagg agcgtgcctc
    37501 atgagcgaaa cttttttcca tctgctgggg ccaggaacgc aaccgaacga tgacagtttc
    37561 agcatgaatc cactgccgat cacctgtcag gtgaatgatg aaccgagtat ggcggccttg
    37621 gagcaatgtg ctcacagccc gcaggtgatt gcgctgttaa acgagttaca acatcaacta
    37681 agcgaacgcc agccgccgtt gggcgaggtg ctggcagtcg atctgttaaa tctcaacgcc
    37741 gacgatcgtc actttatcaa tacgcttctc ggggaagggg aagtgtcagt gcgcattcag
    37801 caggctgacg acagtaaaag tgaaatacag gaggcgatct tctgcggatt atggagggtg
    37861 cgcagacgtc gcggcgaaaa gttgctggag gacaaactgg aggctggctg cgcgccgctg
    37921 gcgttgtggc aggcggcaac gcaaaatctc ttgccgacag attcgctgtt accgccgccc
    37981 attgatggcc tgatgaatgg cctaccgttg gcgcatgagt tactggcaca tgtacgtaac
    38041 cccgacgcgc agccgcacag cattaatctg acgcaattac ccatcagcga ggctgatcgg
    38101 ctttttctct cacgtctctg tgggccggga aatattcaga ttcgtaccat tggctatggc
    38161 gagagctata tcaacgccac ggggttacgc catgtctggc atttacgctg tacggacacc
    38221 ttaaaaggcc cgttactgga aagttatgaa atatgcccaa taccggaagt ggtgctggca
    38281 gcgccagaag atttggtcga ctctgcgcag cggcttagcg aggtatgtca gtggctggcg
    38341 gaagctgcac cgacgtaaaa agacggtaag tatcgctttc agtcttatga atatcgcaat
    38401 cggcgaatac ctctggtcgt agagtttcag gataaagagg gagatctaac attatcgggt
    38461 tatttttctc tcttcgccta caggagtgcg catgtgggat gtcattgatt tatcgcgctg
    38521 gcagtttgct ctgaccgcgc tgtatcactt tttatttgta ccccttacct tggggctgat
    38581 ttttttgctg gctatcatgg aaaccattta cgtggtcacc ggcaaaacaa tctaccgcga
    38641 tatgacgcgc ttctggggta agctcttcgg tatcaatttt gctcttggcg tggctaccgg
    38701 cctgaccatg gagtttcagt ttggtactaa ctggtcattc tattccaact atgtgggcga
    38761 tatttttggc gcaccactgg cgatggaagc attaatggcc ttcttcctcg aatccacctt
    38821 tgtcgggctg ttcttcttcg gctggcaacg gctgaataaa taccagcacc tgctggtgac
    38881 gtggctggtg gcgttcggtt caaatctctc tgcattgtgg atattgaatg ccaacggttg
    38941 gatgcaatac ccgaccggtg cgcattttga tatcgacacc ctgcgtatgg agatgaccag
    39001 cttcagcgag ctggtcttta acccggtcag ccaggtgaaa tttgtgcaca ccgtcatggc
    39061 gggctacgtg accggggcca tgtttattat ggcgatcagc gcctggtatt tactgcgcgg
    39121 acgggagcgc gatgtcgcat tacgctcgtt tgccatcggt tccgtcttcg gtactctggc
    39181 gattatcggt accctgcaac tcggagacag ttctgcgtat gaagtcgcgc aagtacaacc
    39241 ggtaaaactg gcggcgatgg aaggggagtg gcaaacggaa cctgctcctg caccgttcca
    39301 tgtggttgcc tggccggaac aggatcaaga gcgtaacgcc tttgccatca aaattcccgc
    39361 gctgctaggg atcctcgcca ctcactcatt agataaaccc gtgccgggtc tgaagaattt
    39421 gatggctgaa acctacccac gcttgcaacg cggacgtatg gcctggctgt taatgcagga
    39481 aatatctcaa ggcaatcgtg agccgcatgt gttgcaggca ttccgggagc tggaaggcga
    39541 ccttggttac ggcatgttgc tctcccgcta tgcgccggat atgaatcatg tcacagccgc
    39601 acagtaccag gcggcgatgc gtggcgcgat acctcaggtt gcgccggtat tctggagttt
    39661 ccgcatcatg gtgggctgtg gttccctgct gctactggtg atgctgattg tgcttgtcca
    39721 gacgctgcgt ggcaaaatcg accagcatcg ctgggtgctg aaaatggcgc tctggagttt
    39781 gccgttgccg tggattgcga ttgaagccgg gtggtttatg accgagtttg gtcgtcagcc
    39841 gtgggcgata caggacatct taccgacata ctccgcgcac tccgctttaa ccacaggaca
    39901 actggctttc tcactgatca tgatcgtagg gctttacacc ctgttcttaa tcgccgaagt
    39961 ctacctgatg cagaaatatg cccgtctggg gccgagcgcg atgcagagtg aacaaccgac
    40021 gcagcaacag gggtaaagga gaaaatcatg tttgattatg aaacattgcg cttcatctgg
    40081 tggctgctga ttggcgtgat cctggtggtc tttatgatct ccgacggatt tgacatgggg
    40141 atcggctgtt tgctgccgct ggtggcgagt aatgatgatg aacgccggat agtgattaat
    40201 agcgttggtg cgcactggga aggcaatcag gtctggttga tcctcgcagg tggggcatta
    40261 tttgccgcct ggcccagagt gtatgcagcg gcgttttccg gcttttatgt ggcgatgatc
    40321 ctggtgctgt gctcgctgtt cttccgtccg ctggcctttg attatcgcgg aaaaattgcc
    40381 gatgcacgct ggcgtaaaat gtgggatgcc ggtctggtca tcggcagtct ggtgccgccg
    40441 gtagtcttcg gtatcgcctt cggcaacttg ttgctcggcg tgccgtttgc cttcacaccg
    40501 caattacgcg tggagtatct cggcagcttc tggcaactgc tgacgccatt ccctttattg
    40561 tgtggtttgc tcagccttgg gatggtgatt ttgcaaggtg gcgtctggtt acaactgaaa
    40621 actgttggtg tgattcatct gcgttcacag ctggcgacta aacgcgctgc actgttggtg
    40681 atgctgtgct ttttgctggc aggttactgg ctgtgggtcg gtattgatgg ctttgtactg
    40741 ctcgcccagg atgctaacgg tccttccaat ccgttaatga aactggtggc agtgctacct
    40801 ggtgcctgga tgaataattt cgtcgaatcg cccgttttgt ggatcttccc gctgctgggg
    40861 ttcttctgcc cattgctgac ggtgatggcg atttatcgtg gtcgcccggg ttggggattt
    40921 ttgatggcat cattgatgca atttggcgtg attttctcgg caggcatcac gctgttcccc
    40981 tttgtcatgc cgtcaagtgt gagtccaatc tccagcctga cgttgtggga cagcacttcc
    41041 agtcagctga cgctgagcat tatgttggta atcgtgctga tatttttgcc cattgtgttg
    41101 ctctacactc tctggagcta ctacaaaatg tggggacgca tgacaacaga aactctccgc
    41161 cgtaacgaaa acgagttgta ctaaggagca ggaacatgtg gtatttactt tggttcgtcg
    41221 gcattttgtt gatgtgttcg ctctccaccc ttgtgttggt atggctggac ccgcgtctga
    41281 aaagttaacg aacgtaggcc tgatgcggcg cattagcatc gcatcaggca atcaataatg
    41341 ttagatatga aaagcggaaa catatcgatg aaagcgatct taatcccatt tttatctctt
    41401 ctgattccgt taaccccgca atctgcattc gctcagagtg agccggagct gaagctggaa
    41461 agtgtggtga ttgtcagtcg tcatggtgtg cgtgctccaa ccaaggccac gcaactgatg
    41521 caggatgtca ccccagacgc atggccaacc tggccggtaa aactgggttg gctgacaccg
    41581 cgcggtggtg agctaatcgc ctatctcgga cattaccaac gccagcgtct ggtagccgac
    41641 ggattgctgg cgaaaaaggg ctgcccgcag tctgctcagg tcgcgattat tgctgatgtc
    41701 gacgagcgta cccgtaaaac aggcgaagcc ttcgccgccg ggctggcacc tgactgtgca
    41761 ataaccgtac atacccaggc agatacgtcc agtcccgatc cgttatttaa tcctctaaaa
    41821 actggcgttt gccaactgga taacgcgaac gtgactgacg cgatcctctg cagggcagga
    41881 gggtcaattg ctgactttac cgggcatcgg caaacggcgt ttcgcgaact ggaacgggtg
    41941 cttaatttcc cgcaatcaaa cttgtgcctt aaccgtgaga aacaggacga aagctgttca
    42001 ttaacgcagg cattaccatc ggaactcaag gtgagcgccg acaatgtctc attaaccggt
    42061 gcggtaagcc tcgcatcaat gctgacggag atatttctcc tgcaacaggc acaaggaatg
    42121 ccggagccgg ggtggggaag gatcaccgat tcacaccagt ggaacacctt gctaagtttg
    42181 cataacgcgc aattttattt actacaacgc acgccggagg ttgcccgcag tcgcgccacc
    42241 ccgttattgg atttgatcat ggcagcgttg acgccccatc caccgcaaaa acaggcgtat
    42301 ggtgtgacat tacccacttc agtgctgttt attgccggac acgatactaa tctggcaaat
    42361 ctcggcggcg cactggagct caactggacg cttccaggtc agccggataa cacgccgcca
    42421 ggtggtgaac tggtgtttga acgctggcgt cggctaagcg ataacagcca gtggattcag
    42481 gtttcgctgg tcttccagac tttacagcag atgcgtgata aaacgccgct gtcattaaat
    42541 acgccgcccg gagaggtgaa actgaccctg gcaggatgtg aagagcgaaa tgcgcagggc
    42601 atgtgttcgt tggcaggttt tacgcaaatc gtgaatgaag cacgcatacc ggcgtgcagt
    42661 ttgtaatgca taaaaaagag cattcagtta cctgaatgct ctgaggctga tgacaaacga
    42721 agaactgtct aatgcgtaga ccggaaaagg cgttcacgcc gcatccggtc actttcagtt
    42781 ttactctttc tcggagtaac tataaccata atagttatag ccgtaactgt aagcggtgct
    42841 ggcgcgttta atcacaccat tgaggattgc gcctttaata ttaacgccag cctgttccag
    42901 acgctgcatt gacaagctca cctctttggc ggtgttcaag ccaaaacgcg caaccagcag
    42961 gctggtgccg acagagcgtc ccacgaccgc agcatcgctc accgccagca tcggcggcgt
    43021 atcgacaatc accagatcgt aatggtcgtt cgcccattcc agtaattgac gcatccgatc
    43081 gcgcatcagc agttcagacg ggttaggtgg cacctgaccg cgagtaatca catcaaagcc
    43141 tcctttgcca aaatgctgga tcactttgtt gagctcatct ttacctgcca gatattccga
    43201 caagccatgt tcattactca cggtaaacag gttatgcgaa taaccacggc gtaagtcggc
    43261 atcaataaat aacacttttt gatcggactg ggcgatcacc gctgccagag ttgaactgac
    43321 aaacgtttta ccactgtctg gcgtcgcacc ggtgatcatc agaatgttat tctcagtctc
    43381 catcatagcg aaatgcagac tggttcgtag cgcacgtacg gcttccacag cagaatccgc
    43441 cgggttatcc accgccagga aggggatatt tttagtacga tggcgctgct gattagaaaa
    43501 taaatttttc ttacgcagac gggtgcgttt atccagccac tcggacattg ggatagtgga
    43561 ataaacgctg atgccgtgct cttccagttg ttccggggct tctacaccac gacgcaacat
    43621 cgcacgcgcc agcacggcac tcaccgaaat aaacaggcca agaataaaac caagcaccac
    43681 attcaacgct tttttcggtt tcactggctg cggctgagtg actgccgggt cgataatccg
    43741 cacgttacca atggcactgg atttcgaaat actcaactcc tgctggcggt taagtaattg
    43801 cagatatacc gcacggcccg cttctacgtc acgacttaaa cgcaacactt cctgttgggt
    43861 ggaaggcatt gccgataccc gcttattcag gcgtttgcgt tcttgctcca gcgtctggcg
    43921 tttttccagc agcgcacgat aagttgggtg atctttctta tacagcaggg agatctctgc
    43981 ctcgcggaag gtcagctcat tgagttgatt atcaacgttc acaatctgct caagaacggc
    44041 tttggcttcc aggttaaggt caaccgaatc gcgctgctgg cgataaacgt tgagtttttc
    44101 ttccgcctgg tccagctcgc tgcgcacttc aggtaactgg cgctgtaaga attcaaggct
    44161 ttgtgaatcc tgcgccgcct ggcgagcgat attctgttgc aaatagttgt tagcgatgct
    44221 gttcagaata cgagtaatca actggggatc atcaccagtc atggtaagtt ccagcatccc
    44281 gctttcttta ctgcgttcgc taacggtaaa ggtttcctgc aatgcgttaa tcgcttccag
    44341 ttcggtacgc tggctcagga caaactgtgt tcctggtttg gccttaatgt ccgcgatagt
    44401 cagcgcaacg ccatcttttt ccagacgctg gccgaccata ccattgacgg tgaactcttc
    44461 accttccagt gtatagtggc cgttttcccc aaccgtgagt gtcagttgct gatcctgacc
    44521 attcagttgt ggaatatgca tccagctgat cgccagctca cctggttttt ctttggttaa
    44581 tctcgcccag ccgcgaccca caatcggaaa atacttctgc tcaactatgt cgcgcagatt
    44641 cagttcagca atggttttac cgagaatcat gcgcgattgc agcagttgga tctccggtgc
    44701 agactcgggc gatgagttag ggatcatatc gctcaggccg ctgagaatgg cgttgccctg
    44761 tttttgctca acctggacca gagtatctgc ctgataaatt ggtgtgctta acagcgatca
    44821 gcgtgaataa cgcggtcacg ctgataataa acttacggtg atcccataac tcgccgacca
    44881 gacgaagcag atcgatctca ttttcctgag tgctgcctgg tggcgtattc atatttttag
    44941 ttgtcatcgt attttataaa ctcaaattac cggctgaggc gcttcgccca ttcctgactg
    45001 gcgcgctcca acataccgta gacatgttca aatgcgtcct gacttttacg atagggatcc
    45061 gggatctctt tctgttccag ccattgccca aacagcattg tcttcccgcg gacctcggga
    45121 gcaattgcgg taacctgagc aatatgttcc gactccattg ccagaatcag atcgtaattt
    45181 cgtgccatct cagcagtgag cttacgtccg gcatgtcctt caagagatac gccatgatta
    45241 gcggcaacat ctgccgctgt cgcatctgca gggtgtttta ccagaccatg aacacctgcc
    45301 gatttgactt ttacgcccgg cagacgttta cgcagcaagc gctcgccaat gggcgaacgg
    45361 caaatgttgc cggtacaaac cactaggatt gagttaaatt ttagttgggc catgtttgaa
    45421 tccaccgtga agtctctgtc aggtcatgca ccccggaaat cgtcggtacc agcagagaaa
    45481 tcacacggtt ccaacgagcc agcggcgcgg tagtgacata aacaatgtcg taaggttcaa
    45541 gctggaattc cgttccgagt atcattgccg atgcgtcctt cgcattcagc tgataaatat
    45601 tggcgatttt gccattctgc ttgttttgcg ttgcacgaat aacaaaaatc cccgtcgcat
    45661 cagcgacatc ctgattcata ccctcagcgt tacccagcgc ttccgccagg gtcataccgc
    45721 tgcggtccat tttcagcgtg ctctgtttac ccacttcacc catcacgaag actttcagat
    45781 catcgttgcg cggaataaat aaaatatcgc ccgggtgcag cagtttgttc tggcgcaagt
    45841 cgccacgctg catcagcgca tagaggttca ctttggtctt tacgccattc tgggtgagca
    45901 ccacgttgcg ccagtctgca tctgcggtca gaccgcccgc ggcgttaatc gcatccatga
    45961 tggtcagagg aatattggta atcggctgct gacccgattt gctgacttcg ccggtgacgt
    46021 acgctttttg cgatcggaac gccgcgacgc tgacatcgac ttgcgggctt tcaatgactg
    46081 aatcgagacg cgctgtaatc tcgttgcgta cctgggtaag tgttttcccg gccactttca
    46141 ggcgaccaat ataggggtag aaaatcgcac catcggcgtt aacccagttg ccggtatcac
    46201 tggcgctacg gtattgccct gcgggggtgg ttagttccgg gtgatcccag accgtaacca
    46261 tcaacacatc accaatcccg atacgatatt catagtttgc gatttgctgc tccagctccg
    46321 ggttagcctg cgacataatc ggtttggcgc gcagctgatc aatcaatcct ggcgtgacgg
    46381 gatagatatt gaccattttg tccaggtcat actgattgtc aggtagttca atgacatcct
    46441 tgttactggt cgataaattc tgaccaggaa caagggtaca tgctgtcaaa gacagaacag
    46501 ccaatgtcag taccgaaaac ttaaaaatgt tctttttcat tggtttatat ttatcagtca
    46561 atgtaatgtt atgaattacg tcaggttatt gtccaaccgg tacttctcgc tctgccgtca
    46621 ttggatcgag ctggtatttg cgtgacagca tttgtccacc atcacgcgtc aatggtgtcc
    46681 acgaaaccac cgcgcggttg cggttaggtc caatggtcat caagtcgaac ggaatcgaga
    46741 tataaaaacc tttactaaag ccgccttcgc cgtagtcatc tttagatacg ttactgattg
    46801 ccgcccatac ccctaccgcc acgccgctgt caaagcgttt ggcgacgtcg atcgttgcgc
    46861 ctttatcttt tgccagatat tgcccaatgc taagtttcat cagtacgcca ttgagcgtcg
    46921 gcgggttcca gtaagccgtc acgaaacctg tgggcgtgga ataatcggtg aagcgcatca
    46981 tgttgtccca gtcacgctgc ttcacgtagt taacgtccac acccagcgcc cagctggcat
    47041 ctagcgggcg ataaagaagc tcggaaccga caccggcata catcgtttcc agataaccgc
    47101 catacacctg gccatagaaa ccattgccta agtcggcaaa gtaattcgct tgcaagttgt
    47161 tgagataaac gtcattgcga acgtaatcac ggatatgcgt gcgcacgcgg ggcagggtag
    47221 agtccgcggg taacagtgaa gacttaaact tgtcgtagtt gttgtaaata ttggtgaaaa
    47281 taccgccatc aagcagcagg tggtcggtaa accagtagcg ggcgctggac atcagcccca
    47341 gctggaacat atagaaatct tccgggccgc ccagcgactg tgaaagtgtt gggttgaaag
    47401 agtagctaaa gcgatcttca cgaatacggt agccccgacc aaaggcagaa agatcttctg
    47461 cttcaacacg ttgttgttgc agttgctctg atttaccagg cgctgtgccg gccagctgtt
    47521 tgcgcaggct ggctacgtcg gtttcggtag tcaccatcgc catatgctcg cgcttttgcg
    47581 tcacgctaat cttctcaacg ccttgcggca ggttattcac cagaatccgg ttggcacgat
    47641 cgaccgcttc gcgagagtca cggtatttgt attgctgacc agacatatac agcgtcttat
    47701 cgcgtagctg aatttctggc gcgtcaaagc ccgcgttata cttcagggcg gtaagttggt
    47761 ttgctaccgt ggtgtactgc aaaccttcag actctggcgc aggctgccag gccggttttg
    47821 gcgtatcgcg tagggcaggg cgcagatcgt tgaaattggt ccgtaaagtg aaaccaaaca
    47881 tcaacgtgtt tccgcgttca taactgaggt tgaggtctgc ccagctggca gcacgataaa
    47941 ctgcaccgac gttgaaatgg cttgcctgag gcagtttgcc agcgaaatca ttctggtaat
    48001 tgttgccgtc gtattcgagt ttcagacgca ggggattcca cggcgtttga tactcaatgc
    48061 cgccaaagat ggaagccggg ccacgaaaga tatcgctaaa gctgatatcg cccgcatcgt
    48121 gagactctgc gcgatgacaa tatttatcgc tcacccggca aaacgggttg gtaatattgc
    48181 ccgcattacc ggcgtagccc catgccatcc cgagggtgaa atcaaatggc cccgcttgct
    48241 tgctggccac cagatattca ccgtcaaaca aacccgtacc agcaatatca cgtttaccaa
    48301 acgccacttg cggtagccag taaccttctt cccacaggcg aagtttaaaa tcgaatgatt
    48361 tgtctttgta tgactgatcg ccgctgaaat cttcccactg gctatatttg cgtgtgcgca
    48421 catccgtata acgaatggtg ccttccagcc atgggaacag cgccacggag gtggagtaga
    48481 accggtattg atcgttatcc cggtagttga cgctgaattc accttccggt gcgatgcgcg
    48541 catttggcat ctgcaacaat cctgtgccgc caaagtctga ttgcgacgaa cccagcggat
    48601 ccgggtaggt taatacttca gcatgacagg cactggagac ggcaatggcc aggcagctta
    48661 aaagataaga attcttcttc atcagtcagg aacacgttgc gttaaaacag acacaatctg
    48721 gtcatttaaa tcagcatatt tttcgggcag tacatgcgca gaaaagccca accaaagctg
    48781 gctgccaggc ggtggttcca catgtctttt attccacagg gcaacagggg cgacgacagt
    48841 ctcaccctca ggcgtaatca ccataacgtt attcttgtcc gcgcctgcaa ggcgggggtg
    48901 atcttgcaga taatcggtaa cgctacggcc tgcctgccag ggcaattgcc cagccccaga
    48961 aaccgcaccc agcagagtaa tagtcaccgg acgctgtacg gtatagagcg tatagtcccc
    49021 gaccaacggg ggattgctgt tctcatccac tcgtacaaaa tcagggtcca gtttgaccgg
    49081 taagcgacca gtgatattga gattgagtaa ctgctggcgc acggatttaa tcgttgccgc
    49141 cacatcgtcg tcagcttctg cttcccagga agcaagctgt gccatgacat gttggtagtc
    49201 tttaagcgct ttagctttcg ccgcagagtc ggtcagcaag gcaccaggcc accagaggcg
    49261 atcgcgcagt tgcggttgtg tcaccaactg gacaacattt tccacggggc caacggaaag
    49321 tgtctgttgt tcgctaggca gataaatagt caccgttcct tgcgcaaagg catggggtgt
    49381 cattacgtaa agtacgctgg caatgaaata cgactgtaat ttattcatgg tgcgggttta
    49441 agaaacgtca tttctacggg aataacccct gcgcctaaca tttgccgact ttgacgaacc
    49501 tggccagtag ccgaatcgac ccagaatgtg ttttgccact gtttttctgg cagcgtggaa
    49561 gtcacctctt cctgccagac attacacagc acattgtgac ccgcaatatt cagcgtctct
    49621 aatccagcaa aggaaaaagt agaacttagg gtcgcagagc gaaaatggtt gtcttcactc
    49681 cagagaatat cgcgcgtcca gcggctgcca tcttttattg ccagcgcgtt acgcagaggg
    49741 tcccgcccgg gatttgtcac ttccagcaga ttattattaa gtttgacggt ctttaaaaga
    49801 cgtccattgt gggtaaccag catggcgtta tcctgggata accatttgct ttgttcttgt
    49861 tctatatatc ccagtaccac aaagattcgt tgcccttcat taaggcgtaa atacatcgtg
    49921 gaataaggca acgcctggat ctgctgatcc gctacagtga tatcctgatt atcgaaaagg
    49981 ctggcgcgaa atgtatcgac catactttgc tggctgtgcg tacatcccgc aagaaatagt
    50041 gcgaaaatcg ataaaataag agggcgcacc tttaatcctt gaaaggttaa ttaaaaaaat
    50101 gcaaatctct aaagcccggt acgaatatcg caaccgggta ttatttaact tgaatttcga
    50161 cgcgtttaag cagaattagc gcgtagtgga cgtggtggtt gaggtgttat gagaaccgcc
    50221 atcgccactg tcatttgctg caactactgc caacgtcgcc acaccaacag ccgttgccac
    50281 accgacaact gtcgccgtgc cgacagcgcc agcagccacg ccactagtgg tagccgcagt
    50341 cgtggttgca cctgtagtgg tcgttgtggt gccagtagta cccgtcgttg cctcggtccc
    50401 gtttgctgct tcaggggcag caaacgcagc tggcgtcgtc aacatgaagg ccataaggat
    50461 tgcagaaagc ttgtgtttca taacttttcc tttattcatc gcatggacaa tacgtacgct
    50521 tgagatcagg attgtctact ttgagtataa catccttgtg aaatctccct ttcctcatac
    50581 cgctagcact atatactgcg ggtgggaaag gcgcgcagag ggaaatataa gattgttaac
    50641 gatttccctc tggaaatacg acttgtgagc ataagataaa tcttagaaaa atacgaatta
    50701 aagataagaa aattgttacg aaagctatta attattgttg gtaatatagt ttcaagtggt
    50761 actataactg gctattaaat ggcatttata actattaggt gcagagatat tcgcttaaat
    50821 ggattagttt cttggtggaa aagtaactga tgttataatg tatggtgttg actattgaac
    50881 tattccaata aaaccagtca gctttaaaca agcagcgtca tattaagaga gataaacatt
    50941 tgccgctgtt ggtcctcgca ggccatttac gcggcaaaat tccacgcgta atcctggtat
    51001 aagcacttct gcgtcgcggg gagtgaatgc ggaaatatgg acctggactt ctttacgacc
    51061 gtcggagggg ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt
    51121 cattttacgg gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa
    51181 taaaataaag ccagcgatat ttaagaccgc cggacggcta aaataaaatt tgcttaatct
    51241 caattatcat gccttaatag ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt
    51301 actaaagcag ttctcattat caggcattat ccccttcttt tgagtctctc tcctgaacac
    51361 taagtggttt ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat
    51421 atgtctaata aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt
    51481 atcactcctg atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat
    51541 gaattccgca cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt
    51601 ggccccgcgg cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca
    51661 tttccgctgt ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga
    51721 gcgaaaccct ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa
    51781 cgtcgcgctg caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc
    51841 tccctcgtgg agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa
    51901 gcgataacct gttgattatt gaatctttcg gggagatggc ttataacatt ccttacctga
    51961 ccagggtacc gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg
    52021 ttaacagtga atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc
    52081 gcgcaaaaaa tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc
    52141 atcgcacgag aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga
    52201 ttttgtgaac atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat
    52261 cattggcgct tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac
    52321 tcccgcatac attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat
    52381 taaccagcca gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt
    52441 caatgttgag gctaccggac atgactctac gcaagtcata cagccggtac attccactgt
    52501 tcgtacctga attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc
    52561 acaatcgata caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac
    52621 gcccatcagc gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct
    52681 ggcaatcagc aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc
    52741 gagcatttta acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg
    52801 agcgggcatc aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg
    52861 cagaggaata tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag
    52921 atcgccgatt aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt
    52981 gagtaataga ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga
    53041 cggtaactga cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca
    53101 gccttcgata cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg
    53161 cacccaatac cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt
    53221 cgctttacgc caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt
    53281 gcctggccgc cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta
    53341 agttgcatac caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc
    53401 gcgttcaggt ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct
    53461 tcgtgtggaa ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg
    53521 ctacgcattc ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc
    53581 tcgctgtcct ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt
    53641 ttaaataatg ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg
    53701 agttgcgaga catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt
    53761 aactgaccga gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg
    53821 gtacgctggc gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac
    53881 ggatattgct gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa
    53941 tccaccagtg cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca
    54001 ttgccaatag caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg
    54061 gttagcgggt tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg
    54121 gctgtttttg gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt
    54181 ccaacctccg gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta
    54241 ctgatagtca gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg
    54301 aagatttctg ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc
    54361 cattgctcgc catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg
    54421 gcattgctca ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg
    54481 ctcggtacat catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg
    54541 ctcattaatt gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact
    54601 gaaacatttt tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc
    54661 agcaacgatt cgccagagtc ggtaatcgcc cgcaaatcat cacgctgggc gttaagtgcg
    54721 gggttatctg ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca
    54781 tggctcatcg ccgccagaaa cgccgatttt gcctagctgg ctttttctgc ttccgcccgt
    54841 gcctgtcggt gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc
    54901 gccagctgtt cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc
    54961 cgcccgatgg tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca
    55021 ccgtccagca gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gactgagcga
    55081 taaaccacgc gccagaggat cagaatcagt gcgcaaagtg aaaccatccc cagcaataac
    55141 aggctgtatt gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgcgc
    55201 agctcagtgg tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata
    55261 ttattttggg cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc
    55321 aacagatcgc tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc
    55381 ggatcttcaa tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt
    55441 tccagcgttg gtgcattttt ctggagctgc tccagcccca gattcatcac catttgctgc
    55501 acccgcagag cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca
    55561 atcagccgat cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa
    55621 atcccggcct gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgcgccagg
    55681 cgtgcgatct catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt
    55741 tgcagacgcc gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc
    55801 tgttgttcaa tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta
    55861 atcttcaggc tttgtgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg
    55921 gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc
    55981 gccatattca tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag
    56041 cgcaggttgt accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag
    56101 cccatccaga gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag
    56161 gttgagtcgc aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg
    56221 cgcgtactgc tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac
    56281 ctgttgcgct ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc
    56341 aaacgcgtat ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg
    56401 ttgaactatg gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag
    56461 gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg
    56521 ggcgcagagg ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag
    56581 gtagcaaatc tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc
    56641 cgcgttggtg taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg
    56701 gcgcacggta acccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt
    56761 aaggagatgg tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat
    56821 attgcgcttg gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa
    56881 cgccacccag aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt
    56941 gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg
    57001 tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag
    57061 ggggaactgg cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat
    57121 gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc
    57181 tcgctgtcac cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag
    57241 aaataacctt caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga
    57301 cgaattaaca catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata
    57361 cgcagcagac gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt
    57421 tcatactctg cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac
    57481 acattcaggc aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt
    57541 cgcgcgaggt cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc
    57601 agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga
    57661 tcgctgcgtc cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt
    57721 aacatcaggc cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga
    57781 ttctgcataa tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc
    57841 tgagtgaagt aggattgtaa tcgcgcctgg gtaaccggct catcttcaac aataacaatg
    57901 tgatgtggca tcagagggtt ttactcattc tgttcatatc tgttcatatt ctgccgtaag
    57961 ccgttcatcc tgaccagtgc cgctgttcat atttgctcat taagatcgct tcactaaacc
    58021 ataattctac aggggttatt atgcggaaac tctggaacgc gctacgccga cccagtgctc
    58081 gttggtcggt actggcgctg gtcgcaattg ggattgtgat tggcattgcg ctgattgtat
    58141 tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta
    58201 tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc tccggcgtgc
    58261 gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac
    58321 tggaagcgag caacgacatt taccagacct ttattgccca ctccattgat acacctgaaa
    58381 aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca
    58441 actcggcaac ctgtcgctcc tgccataact acgatgcgat ggatcatgcc aggcagcatc
    58501 ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc
    58561 ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg
    58621 agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat attaagccaa
    58681 tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct tcggaagtga
    58741 aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg acggaaagcg
    58801 ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc
    58861 gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac caccgttgcc gacaccaata
    58921 ccgagtggag caagttgcag gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata
    58981 tcaaaccgat ctgggcctat gcggattcgt tgtacaacgg cacctgtaac cagtgccacg
    59041 gcgcaccgga aatcgcccac tttgacgcta acggttggat cggcacgctc aacggcatga
    59101 ttggctttac cagtctcgat aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga
    59161 atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa
    59221 taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt
    59281 cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc
    59341 gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg
    59401 cgcgacggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc
    59461 gtcgaaaatg atcgccggat tgccggatca cgtacacaac gcggcacgta ttcgttatcc
    59521 gatggtacgc gtggactggc tgcgtaagcg ccatctgagc gacacctccc agcgcggtga
    59581 taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga
    59641 acgcgtacag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac
    59701 ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag
    59761 cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt
    59821 cggttcaatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa
    59881 aaccattgtg ctgtggggct ccgatttgct gaaaaaccag caagcgaact ggtggtgccc
    59941 ggatcacgat gtttatgaat attacgagca gctaaaagcg aaagtcgccg ccggtgaagt
    60001 tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca
    60061 tgtgaagcac attgcggtta acccgcaaac tgacgtgccg ctgcaactgg cgctggcaca
    60121 tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtgggttt
    60181 tgagcagttc ctgccgtatc tgctgggtga gaaagacggt cagccgaaag atgccgcatg
    60241 ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt gggctggcgc ggcagatggc
    60301 ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca
    60361 gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg
    60421 tggttttggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
    60481 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
    60541 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
    60601 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
    60661 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
    60721 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
    60781 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
    60841 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg cagttcgagg cgcgcaacga
    60901 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
    60961 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
    61021 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
    61081 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
    61141 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
    61201 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
    61261 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
    61321 gcagttatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
    61381 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
    61441 ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct atgcacccgg
    61501 cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    61561 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcggtactt cgcagctggc
    61621 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gaacagtgga
    61681 gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc
    61741 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    61801 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    61861 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcggtgaat
    61921 gatctggaaa acagtattgc cacgctgaca gttcgtgacg atgcccgtct ggaactggcc
    61981 gctgactttt gcggcctgtt tctgatgacc gacaaacaag tggcgctgcc gtatgcatcg
    62041 gcctacaaac aggacgagca agagattaaa cgcttgttag ttgaggcagg gatggaaacc
    62101 agtggcaatt tcaacgaacc ggcagatcat ctggcgatct atctcgagtt gttgagtcat
    62161 ctgcattttt cgctgggaga ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa
    62221 aaaacactga cggcgctgcg gcaatggtta ccagagtttg ctgcgctttg tcgacagtat
    62281 gacagctttg gtttttacgc ggcactaagc cagttattgc tggtgttagt ggagtgcgac
    62341 caccaaaaca gataacgtcg tttatgcgcc tgaaaagacg cgtttagcgt cgcatcaggc
    62401 attatggcgc agttgccgga tgcgtcatca catccggcaa acacacatca cggatgagct
    62461 acaaaccggg aaagccgctg gcgtagcagg cggttttcct gcttcaggtg cgcaatatca
    62521 tccattaacg tcagcgccac cgcgatcccc ggccagtcca gagccagttc atgacgcagg
    62581 cgtaccgcgc gttgcaccac aatggcggca tggtcgtcaa atacccaggt tgtttcctgg
    62641 atctcacgcg gttcaaccac ccccaaaccg acaatttcat tcaactcctc ttcagagatg
    62701 ccggtatgca ggcaaaattc ggtaatagta aaagtcaccg taacattagc cattatgctt
    62761 tcccccaatc tttacgtgga tcaaaagacg actgggcgtc tgccagttgc tgccacagcg
    62821 cggcagtgtt ttcatccggt ttcggcggca tcacgatttt cagtaccgca tacagatcgc
    62881 cggtctgttt tttgctcacc agacctttgc ctttaacgcg caatcgttgc ccggcctggc
    62941 tgcctggcgg gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg actttagtac
    63001 ccagcgccgc ttcccacggg ctaaccggca ccacaatttc cagatcctgg ccgacaatat
    63061 cgaacagcgg atgtggcgca atatgaatca ccagccacaa atcgccattt ggaccgccgt
    63121 tttcgcccgg cgccccctgg cctttcaggc ggatgcgttg accattgccg acgcctgccg
    63181 ggatcttcac attcagcgtt ttcgggattt cctgttcgat catgccaaag gcgttataaa
    63241 ccggcaggtt atagctgatg gtacgcttat gctcagtaag cgtttcttcg aggaataccg
    63301 ccacttcgat ttcaatatcg tggccgcgtg cggcggggcg ttgacggctc tggcgggcat
    63361 gctgaccgaa aattgacgag aagatatcgt caaaatcttc ggcattaaaa ctctgaccgt
    63421 cgccatgatg gaactgacgg ttaaattgcg gatcgttgcg atgttgccac atctgatcat
    63481 actcagcgcg acgttgttca tcacttaaca cttcccaggc ttcagcgacc tctttgaagc
    63541 gggcttcggc atccggttct ttgctgacat caggatggta tttacgggcc agtcgacgat
    63601 aggcggtctt gattgtcttg agatcgtccg tcggtttcac gcccatgatg gcgtaataat
    63661 cctttaattc catagcgtta tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag
    63721 gtaactccta taagtgtagg gtaatcctca aaatttcata tgccaacaca gaatatgtta
    63781 ttgaaatcat cgcggagagg aggtcgccat caagatgggt tgctgaacat attttaaaca
    63841 ggtgaaaaag ggtgagcgat ttttgatagt tgaaccaggc actttaagtt
//
LOCUS       DADLSB010000006        62422 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.006, whole genome shotgun sequence.
ACCESSION   DADLSB010000006 DADLSB010000000
VERSION     DADLSB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.006"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            216..719
                     /locus_tag="JN331_000370"
     CDS             216..719
                     /locus_tag="JN331_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAY7258605.1"
                     /translation="MGMIGYFADIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(786..2243)
                     /gene="nuoN"
                     /locus_tag="JN331_000371"
     CDS             complement(786..2243)
                     /gene="nuoN"
                     /locus_tag="JN331_000371"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY7258606.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2250..3779)
                     /gene="nuoM"
                     /locus_tag="JN331_000372"
     CDS             complement(2250..3779)
                     /gene="nuoM"
                     /locus_tag="JN331_000372"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY7258607.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3943..5784)
                     /gene="nuoL"
                     /locus_tag="JN331_000373"
     CDS             complement(3943..5784)
                     /gene="nuoL"
                     /locus_tag="JN331_000373"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY7258608.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5781..6083)
                     /gene="nuoK"
                     /locus_tag="JN331_000374"
     CDS             complement(5781..6083)
                     /gene="nuoK"
                     /locus_tag="JN331_000374"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY7258609.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6080..6634)
                     /gene="nuoJ"
                     /locus_tag="JN331_000375"
     CDS             complement(6080..6634)
                     /gene="nuoJ"
                     /locus_tag="JN331_000375"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY7258610.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6646..7188)
                     /gene="nuoI"
                     /locus_tag="JN331_000376"
     CDS             complement(6646..7188)
                     /gene="nuoI"
                     /locus_tag="JN331_000376"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY7258611.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7203..8180)
                     /gene="nuoH"
                     /locus_tag="JN331_000377"
     CDS             complement(7203..8180)
                     /gene="nuoH"
                     /locus_tag="JN331_000377"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY7258612.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8177..10903)
                     /gene="nuoG"
                     /locus_tag="JN331_000378"
     CDS             complement(8177..10903)
                     /gene="nuoG"
                     /locus_tag="JN331_000378"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY7258613.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10956..12293)
                     /gene="nuoF"
                     /locus_tag="JN331_000379"
     CDS             complement(10956..12293)
                     /gene="nuoF"
                     /locus_tag="JN331_000379"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY7258614.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12290..12790)
                     /gene="nuoE"
                     /locus_tag="JN331_000380"
     CDS             complement(12290..12790)
                     /gene="nuoE"
                     /locus_tag="JN331_000380"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY7258615.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12793..14595)
                     /gene="nuoC"
                     /locus_tag="JN331_000381"
     CDS             complement(12793..14595)
                     /gene="nuoC"
                     /locus_tag="JN331_000381"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY7258616.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPTAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14689..15351)
                     /locus_tag="JN331_000382"
     CDS             complement(14689..15351)
                     /locus_tag="JN331_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY7258617.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15367..15810)
                     /gene="nuoA"
                     /locus_tag="JN331_000383"
     CDS             complement(15367..15810)
                     /gene="nuoA"
                     /locus_tag="JN331_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY7258618.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16441..17379)
                     /gene="lrhA"
                     /locus_tag="JN331_000384"
     CDS             complement(16441..17379)
                     /gene="lrhA"
                     /locus_tag="JN331_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY7258619.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKSGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18299..19516
                     /locus_tag="JN331_000385"
     CDS             18299..19516
                     /locus_tag="JN331_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY7258620.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19600..20199
                     /gene="yfbR"
                     /locus_tag="JN331_000386"
     CDS             19600..20199
                     /gene="yfbR"
                     /locus_tag="JN331_000386"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7258621.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20258..22090)
                     /locus_tag="JN331_000387"
     CDS             complement(20258..22090)
                     /locus_tag="JN331_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY7258622.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASTAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22177..22827)
                     /gene="hxpA"
                     /locus_tag="JN331_000388"
     CDS             complement(22177..22827)
                     /gene="hxpA"
                     /locus_tag="JN331_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY7258623.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(22838..23332)
                     /locus_tag="JN331_000389"
     CDS             complement(22838..23332)
                     /locus_tag="JN331_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY7258624.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23415..23870)
                     /locus_tag="JN331_000390"
     CDS             complement(23415..23870)
                     /locus_tag="JN331_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY7258625.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24208..25410
                     /gene="ackA"
                     /locus_tag="JN331_000391"
     CDS             24208..25410
                     /gene="ackA"
                     /locus_tag="JN331_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY7258626.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25485..27629
                     /gene="pta"
                     /locus_tag="JN331_000392"
     CDS             25485..27629
                     /gene="pta"
                     /locus_tag="JN331_000392"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7258627.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEAPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27819..29339
                     /gene="yfcC"
                     /locus_tag="JN331_000393"
     CDS             27819..29339
                     /gene="yfcC"
                     /locus_tag="JN331_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY7258628.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29372..29914)
                     /gene="yfcD"
                     /locus_tag="JN331_000394"
     CDS             complement(29372..29914)
                     /gene="yfcD"
                     /locus_tag="JN331_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY7258629.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29972..30522)
                     /gene="yfcE"
                     /locus_tag="JN331_000395"
                     /pseudo
     CDS             complement(29972..30522)
                     /gene="yfcE"
                     /locus_tag="JN331_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
     gene            complement(30578..31222)
                     /gene="yfcF"
                     /locus_tag="JN331_000396"
     CDS             complement(30578..31222)
                     /gene="yfcF"
                     /locus_tag="JN331_000396"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY7258630.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31358..32005
                     /gene="yfcG"
                     /locus_tag="JN331_000397"
     CDS             31358..32005
                     /gene="yfcG"
                     /locus_tag="JN331_000397"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY7258631.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKWLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32062..32424
                     /gene="folX"
                     /locus_tag="JN331_000398"
     CDS             32062..32424
                     /gene="folX"
                     /locus_tag="JN331_000398"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY7258632.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32445..33338
                     /locus_tag="JN331_000399"
     CDS             32445..33338
                     /locus_tag="JN331_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY7258633.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33386..34276)
                     /locus_tag="JN331_000400"
     CDS             complement(33386..34276)
                     /locus_tag="JN331_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7258634.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34473..35246)
                     /gene="hisP"
                     /locus_tag="JN331_000401"
     CDS             complement(34473..35246)
                     /gene="hisP"
                     /locus_tag="JN331_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY7258635.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35254..35970)
                     /locus_tag="JN331_000402"
     CDS             complement(35254..35970)
                     /locus_tag="JN331_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7258636.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILILHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(35967..36653)
                     /locus_tag="JN331_000403"
     CDS             complement(35967..36653)
                     /locus_tag="JN331_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7258637.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(36743..37525)
                     /gene="hisJ"
                     /locus_tag="JN331_000404"
     CDS             complement(36743..37525)
                     /gene="hisJ"
                     /locus_tag="JN331_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY7258638.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(37746..38528)
                     /gene="argT"
                     /locus_tag="JN331_000405"
                     /pseudo
     CDS             complement(37746..38528)
                     /gene="argT"
                     /locus_tag="JN331_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
     gene            complement(38794..39363)
                     /locus_tag="JN331_000406"
     CDS             complement(38794..39363)
                     /locus_tag="JN331_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7258639.1"
                     /translation="MKRLIVGISGASGAIYGVRVLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39458..40975)
                     /gene="purF"
                     /locus_tag="JN331_000407"
     CDS             complement(39458..40975)
                     /gene="purF"
                     /locus_tag="JN331_000407"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY7258640.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGVIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41012..41500)
                     /gene="cvpA"
                     /locus_tag="JN331_000408"
     CDS             complement(41012..41500)
                     /gene="cvpA"
                     /locus_tag="JN331_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY7258641.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(41759..42421)
                     /gene="dedD"
                     /locus_tag="JN331_000409"
     CDS             complement(41759..42421)
                     /gene="dedD"
                     /locus_tag="JN331_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY7258642.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPLAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42411..43679)
                     /gene="folC"
                     /locus_tag="JN331_000410"
     CDS             complement(42411..43679)
                     /gene="folC"
                     /locus_tag="JN331_000410"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY7258643.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELLE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(43749..44663)
                     /gene="accD"
                     /locus_tag="JN331_000411"
     CDS             complement(43749..44663)
                     /gene="accD"
                     /locus_tag="JN331_000411"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY7258644.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(44819..45478)
                     /locus_tag="JN331_000412"
     CDS             complement(44819..45478)
                     /locus_tag="JN331_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7258645.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45561..46373)
                     /gene="truA"
                     /locus_tag="JN331_000413"
     CDS             complement(45561..46373)
                     /gene="truA"
                     /locus_tag="JN331_000413"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY7258646.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46373..47386)
                     /locus_tag="JN331_000414"
     CDS             complement(46373..47386)
                     /locus_tag="JN331_000414"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7258647.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47452..48588)
                     /gene="pdxB"
                     /locus_tag="JN331_000415"
     CDS             complement(47452..48588)
                     /gene="pdxB"
                     /locus_tag="JN331_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY7258648.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQVRLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48687..49682
                     /locus_tag="JN331_000416"
     CDS             48687..49682
                     /locus_tag="JN331_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY7258649.1"
                     /translation="MIQPISGPPPRQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49679..50857)
                     /locus_tag="JN331_000417"
     CDS             complement(49679..50857)
                     /locus_tag="JN331_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7258650.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQSLTGALTWGLGIVGLKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51132..52352)
                     /gene="fabB"
                     /locus_tag="JN331_000418"
     CDS             complement(51132..52352)
                     /gene="fabB"
                     /locus_tag="JN331_000418"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY7258651.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            52511..54517
                     /gene="mnmC"
                     /locus_tag="JN331_000419"
     CDS             52511..54517
                     /gene="mnmC"
                     /locus_tag="JN331_000419"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY7258652.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLFDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54638..54916)
                     /locus_tag="JN331_000420"
     CDS             complement(54638..54916)
                     /locus_tag="JN331_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY7258653.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(54950..55498)
                     /locus_tag="JN331_000421"
     CDS             complement(54950..55498)
                     /locus_tag="JN331_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY7258654.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55498..56307)
                     /locus_tag="JN331_000422"
     CDS             complement(55498..56307)
                     /locus_tag="JN331_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY7258655.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEYRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56307..57131)
                     /gene="mepA"
                     /locus_tag="JN331_000423"
     CDS             complement(56307..57131)
                     /gene="mepA"
                     /locus_tag="JN331_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY7258656.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57135..58220)
                     /gene="aroC"
                     /locus_tag="JN331_000424"
     CDS             complement(57135..58220)
                     /gene="aroC"
                     /locus_tag="JN331_000424"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY7258657.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58255..59187)
                     /gene="prmB"
                     /locus_tag="JN331_000425"
     CDS             complement(58255..59187)
                     /gene="prmB"
                     /locus_tag="JN331_000425"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7258658.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNQWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTCRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59353..59904
                     /gene="smrB"
                     /locus_tag="JN331_000426"
     CDS             59353..59904
                     /gene="smrB"
                     /locus_tag="JN331_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY7258659.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60008..60898)
                     /locus_tag="JN331_000427"
     CDS             complement(60008..60898)
                     /locus_tag="JN331_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY7258660.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSVAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(60885..61409)
                     /locus_tag="JN331_000428"
     CDS             complement(60885..61409)
                     /locus_tag="JN331_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7258661.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALSGFL
                     AFDSSSSASGAGIGIETAAGTSVPVNNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61406..61876)
                     /locus_tag="JN331_000429"
     CDS             complement(61406..61876)
                     /locus_tag="JN331_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7258662.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(61873..62421)
                     /locus_tag="JN331_000430"
     CDS             complement(61873..62421)
                     /locus_tag="JN331_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7258663.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
BASE COUNT        15547 a        17017 c        15625 g        14233 t
ORIGIN      
        1 tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg aattatcaga
       61 aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa taataaaaat
      121 ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg tctgttttaa
      181 gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc tattttgcag
      241 acattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct ttgatggata
      301 atatacatga cacgctttca ggattacgcc gactggatat tgataaacgc tgggattttt
      361 tacactttgg gctgacaggc acctctgctt ttgatcccgc gaagaatgat ccgctgagtc
      421 gtgccgtgct tgatgaacac agcctcgaag atggcataga tggcttcctg ggattgacct
      481 ggaatcagga attagccgcg acaatcgacc gactggaatc actggatcga agtgagctgc
      541 gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga gtgacctttt
      601 cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa aagctgatta
      661 gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt gttgggtgat
      721 ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac ttacaaacta
      781 acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg tggccataca
      841 cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc gctgtactgc
      901 cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag gctcaccgcc
      961 acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc caccagccac
     1021 cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc
     1081 atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag gatcggacga
     1141 tgccagaaca aaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg
     1201 gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa
     1261 acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc caccagcaga
     1321 tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt ctggctcaac
     1381 gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac tacgcgaatc
     1441 gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc gaagatagcg
     1501 attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct
     1561 ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac aatcatcatg
     1621 ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccagc
     1681 gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa cagcaggaaa
     1741 gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg
     1801 cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat accgaggaac
     1861 agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc aattaacacc
     1921 aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta
     1981 caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg
     2041 cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag cgaaaccagc
     2101 gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca
     2161 atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc
     2221 aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca
     2281 aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag
     2341 aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac
     2401 atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgc
     2461 aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatt
     2521 accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg
     2581 ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc
     2641 ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga
     2701 ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg gattaccgcg
     2761 ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc catgtgggaa
     2821 accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat ccacgcaccg
     2881 tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc cgacgcgttc
     2941 gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag caagatcccc
     3001 gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc
     3061 agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat caacagatat
     3121 tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg
     3181 gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac cagaccactc
     3241 gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct
     3301 ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag
     3361 aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc
     3421 aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca tagtaccgcc
     3481 agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc aatggcgaga
     3541 tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga
     3601 attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc
     3661 agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc aaagcgttcg
     3721 gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg tagtaacatg
     3781 gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac
     3841 agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag cttatccgac
     3901 ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa
     3961 cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc gcagataacc
     4021 gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg ggatgttcat
     4081 cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa
     4141 cactttgtcg tacagccagt caaagcccca ggcgttgtac caccaggtac tcagcaggcg
     4201 gcccggcgca ctgttggcga tggaggtcac cagagtacgt ttacccagcc acagccaggc
     4261 tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct
     4321 gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc
     4381 gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt
     4441 cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt
     4501 gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga tatgaccatt
     4561 cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggcag
     4621 tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg
     4681 cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga
     4741 tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc
     4801 ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc
     4861 gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac
     4921 cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca ggccgtgggt
     4981 acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca gcgcggagac
     5041 aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact gcgcagattt
     5101 accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc
     5161 aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca gttcgttgta
     5221 aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt
     5281 cattgccgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca
     5341 caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga gaaccagaac
     5401 taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc
     5461 gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga ccaccgagag
     5521 catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt cgcctaccga
     5581 catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga aatcaacgcc
     5641 gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac
     5701 gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat
     5761 aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc
     5821 tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg
     5881 aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg
     5941 accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc
     6001 agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc
     6061 agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt
     6121 tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca
     6181 gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg
     6241 taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg ttaacaccga
     6301 ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg ccaatccaca
     6361 cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccaag ttgagcatca
     6421 tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac
     6481 cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc gaaataatca
     6541 ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg
     6601 ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct
     6661 cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc
     6721 catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag
     6781 atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt
     6841 taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga
     6901 gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg ctttttgcag
     6961 cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc aacgctcttc
     7021 gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg
     7081 ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat
     7141 actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc
     7201 ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc
     7261 ggcaggcaga ttttccagcc gaaggacatt acctggtcat aacgcggacg cggtaacgac
     7321 gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat
     7381 ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa tgcagagatg
     7441 gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc ggaatattca
     7501 atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga
     7561 cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gatgacgttc
     7621 cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc
     7681 acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc
     7741 atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa
     7801 accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc
     7861 caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa ggcaatcatc
     7921 ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac
     7981 attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtcca
     8041 taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccacag
     8101 gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt
     8161 tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca
     8221 gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc gtcagtcctt
     8281 cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgcgtgtac
     8341 ctgcgttcac acccaacttc gcggcatccg ctgggttgag cttgatgtac ggctgcggca
     8401 tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtagt
     8461 acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt
     8521 aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt
     8581 tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggcgccc
     8641 aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc atcgagaagg
     8701 tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca
     8761 tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga
     8821 atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc gccacaacag
     8881 cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag gtgctgtgca
     8941 gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa taagccggat
     9001 cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta ccgtcgcttt
     9061 cagcaaagct ggcagcggaa agcaccagat gggcgttttc catgatcgct gtgcgttgat
     9121 gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg gtagcagaag
     9181 cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg gtttccagtt
     9241 cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg
     9301 aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt
     9361 tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata atcaacggtt
     9421 tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc tcaggttcga
     9481 taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg
     9541 cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgagtgtcat
     9601 caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa
     9661 tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct ttcaccgcct
     9721 gacgcactgc cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta
     9781 ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca
     9841 ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg tagaagtttt
     9901 cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa
     9961 taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg
    10021 cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga cgatccttca
    10081 ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac
    10141 cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac
    10201 agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt
    10261 aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac
    10321 cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg
    10381 cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc acacagcggt
    10441 agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc tgattacggt
    10501 gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct
    10561 gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg ttggtcatta
    10621 accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg
    10681 tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt
    10741 tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc gccggatgcc
    10801 agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc
    10861 cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt
    10921 tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc
    10981 ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat
    11041 tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca
    11101 cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc gatatcgccc
    11161 ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg
    11221 cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag gttacgcacc
    11281 agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctg
    11341 cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc ggtcaggaag
    11401 tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg cataccaccg
    11461 gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca cagtcccgga
    11521 tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg
    11581 ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg
    11641 ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg gggtttcgag
    11701 cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc cccgcagatg
    11761 tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc cataatgttt
    11821 ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt tactgccgct
    11881 tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag
    11941 atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg gtctttatag
    12001 gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct
    12061 ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg ccctttcaga
    12121 ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc
    12181 gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg tttgtcatcg
    12241 cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc
    12301 gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc tcatcgatca
    12361 tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc gtaaagcggc
    12421 catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc gcctgaatac
    12481 cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca tggcgaccaa
    12541 ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg cttgccggaa
    12601 tacccagcac atcggcgatc gcgtggatcg caccatcagg cacccagcca cgctgcttct
    12661 gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt
    12721 gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat
    12781 tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac
    12841 aatcaggtca gacaccaggc tgccgcggat cgccgtcgga atttgctgca aatgcgcata
    12901 actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg tcaggtagta
    12961 actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg
    13021 cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc
    13081 tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt
    13141 gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg
    13201 ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc
    13261 agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc
    13321 agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc
    13381 tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca
    13441 gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg ctacgccgcc
    13501 aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca gatcgtaaat
    13561 tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa
    13621 ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca taacgcgaat
    13681 gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg
    13741 catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa tgtagctgtg
    13801 ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac
    13861 gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc cgtgcgccga
    13921 cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat
    13981 cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt
    14041 cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc
    14101 tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc
    14161 aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt
    14221 cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc
    14281 gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt
    14341 gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt
    14401 ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc
    14461 ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat
    14521 taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt
    14581 catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa
    14641 tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc
    14701 aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc gctctgattg
    14761 catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt
    14821 gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca
    14881 gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat
    14941 atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt ttggttccag
    15001 catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac
    15061 caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa aacgcgccac
    15121 gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt
    15181 atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa
    15241 cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg
    15301 gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc
    15361 tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg
    15421 tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag
    15481 taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga
    15541 ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac
    15601 cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc
    15661 aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag
    15721 catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc
    15781 gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc
    15841 actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt
    15901 acaatcactg tagaaattct tttatacagc taattgatgt ggtcttttac tcctttctat
    15961 aaccttttgt caactttaac aaaagtttct tcacattagt ttacataata tcaacaccat
    16021 tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt agatgttgtt
    16081 gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg
    16141 tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt tgttattcca
    16201 taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca ggtgattttt
    16261 ttcaggcgat tatttgtgcg ttcgggacgt gaatctctgg tgattgaaaa ataaacagtt
    16321 ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc tggggtttaa
    16381 aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat cgtccgtcga
    16441 ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag acattgggct
    16501 gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt
    16561 attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc
    16621 tactccactc agcacacgca ggtccgggct catcatctca accggccttg ccgtcacgcc
    16681 aagaccggat ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg
    16741 ccatggaata tctgctttat taagcgtcgc cagcaccata tcgcgaaacg gacttggatc
    16801 atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg ctgcgcagta
    16861 ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac gatgggtggt
    16921 taccatcaaa tccacttcct gggattccag catttctgcc atataggcat tacgtttaac
    16981 gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa
    17041 gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa
    17101 cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag
    17161 ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc
    17221 aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc
    17281 ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc
    17341 gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc
    17401 tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc
    17461 tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa
    17521 aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg
    17581 gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg tgtttgtata
    17641 aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata cgcgaacacg
    17701 tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat gcgagcattt
    17761 taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca aagttatgtt
    17821 atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt tgaatattaa
    17881 ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag tttcacacct
    17941 tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta atgcggaaat
    18001 atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca attatcttac
    18061 agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata acattgcatg
    18121 taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac aatcgagcct
    18181 tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg ggagtacatt
    18241 gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt caagattcat
    18301 gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc gtggtccggt
    18361 gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac tgaacatcgg
    18421 caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg tgatacgcaa
    18481 cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc gtaaagccat
    18541 catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata tttacatcgg
    18601 caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca gcggggacga
    18661 aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc tttccagcgg
    18721 taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc tcgatgatat
    18781 tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa ataacccaac
    18841 cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac gtcagcataa
    18901 tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg ctgagcatca
    18961 ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac tgtcgaaaac
    19021 gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga aaaaacacgc
    19081 caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg ctaacgttcc
    19141 tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg aatttattac
    19201 ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca acgatattcc
    19261 gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa tcgacgccaa
    19321 acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc aggaaaaagt
    19381 tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc gcattgtcac
    19441 gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt tcctttctgg
    19501 ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag tgttatcccg
    19561 ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag ccattttttt
    19621 gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa cgtgcggacg
    19681 gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct ggcagctatc
    19741 aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact ggcgatgtac
    19801 cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata cttcaattcg
    19861 caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact ggtcgatatg
    19921 gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc atatagcgat
    19981 gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa atgtctggaa
    20041 gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga agcgacgctt
    20101 gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc cagcttccat
    20161 ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt ctgcgtcgca
    20221 tcaggcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac agcatcggga
    20281 tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac ttcacaaagt
    20341 cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa gacaccggtg
    20401 tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa ggcgacactc
    20461 ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc gcggtattag
    20521 agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc atatgtggcc
    20581 cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct cccgttttct
    20641 gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc cagtgaatgg
    20701 atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg caggcgatga
    20761 tagcggcaac aggattagga atttcatctg tcagcattaa cgccaccatt agcaccagac
    20821 agaaaatggc atggggtgcc tggctgtgcg cggtcgatgc ttcactcacc tcttccggta
    20881 agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt ttccagttac
    20941 ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa ccttccagcg
    21001 ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga aaaccaattt
    21061 cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct gccatgccca
    21121 catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc agcaattgct
    21181 cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc aaaacgtcac
    21241 gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa cgccgccagc
    21301 gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca tccagccgtt
    21361 gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg gtcagtcgat
    21421 attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc ggggtctggg
    21481 tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc accagcacga
    21541 ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca cgcagcaatt
    21601 cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg ctaatcagcc
    21661 cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc atggcaacgc
    21721 ttaacaccac gggaataaag atagcgacaa cgccggttga actcataaac gcgccaagcc
    21781 ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc gccactttga
    21841 ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg ccaataataa
    21901 acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact tcggggaccg
    21961 tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca tccatacgca
    22021 ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc caaatcaatt
    22081 caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc cataaaaaag
    22141 ccccgacgag acgaggctaa atcatgatca ggtgtttcac tgaataataa catcgccatt
    22201 tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga cctcattcag
    22261 gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg ccaggccaga
    22321 aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa gccccagcag
    22381 ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc gctcagcggt
    22441 tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg ctaccggcat
    22501 ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat tgagtaaggc
    22561 gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt gctccagacg
    22621 cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc gcaaagaggt
    22681 gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc catgacgtct
    22741 ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg ttccatcaag
    22801 atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata atttgattaa
    22861 tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta agcatgcgct
    22921 gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg tcaaaatggg
    22981 tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt gcttcagtgg
    23041 cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg tcctgcaaat
    23101 tagaccagga aacatgcaac gcatgataca tctccataat gtcgatgata gtgcggcagg
    23161 tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat ccgtaaccac
    23221 gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg agcatagtca
    23281 tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc attgttgact
    23341 cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct tttatatgcg
    23401 taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt gaaggcgcgc
    23461 ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc caacacctgt
    23521 ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc agggggtaat
    23581 ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact taaggcgaac
    23641 agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca gcagagagta
    23701 aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat aacgcgattt
    23761 tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta atgctgtccg
    23821 cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat gcttcacctc
    23881 aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg gacaacgttc
    23941 aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga aggcctaagt
    24001 agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg aaaattacgc
    24061 aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc agtgcatgat
    24121 gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg acgttttttt
    24181 agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt tctgaactgc
    24241 ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga gtacctttct
    24301 ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat ggacggcaat
    24361 aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa ctttatcgtt
    24421 aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat cggtcaccgt
    24481 atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc tgttattcag
    24541 ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct gatcggtatc
    24601 gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt atttgacacc
    24661 gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta caacctgtac
    24721 aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta tgtaacccag
    24781 gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac ctgccacctg
    24841 ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac ctctatgggt
    24901 ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga tccggcgatc
    24961 atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa actgctgacc
    25021 aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta tgttgaagac
    25081 aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca ccgcctggcg
    25141 aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt tgtattcact
    25201 ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa actgggcgtg
    25261 ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa atctggtttc
    25321 atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga actggttatc
    25381 gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc tggcggtgct
    25441 gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc cgtattatta
    25501 tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc gtgatccgtg
    25561 caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag ccgcgtaccg
    25621 gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc accacgacgg
    25681 ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat cagaaagatg
    25741 tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct gaagtcgttc
    25801 tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg aactacgaaa
    25861 tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact gacaccccgg
    25921 aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc aaaaacacca
    25981 acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag ggtcgtactc
    26041 gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac aatgttgatc
    26101 cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg tggagctttg
    26161 acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc atcatcaacg
    26221 aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc agcattccgc
    26281 acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac cgtcctgacg
    26341 tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc ctgctgctga
    26401 ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct ttcgctaccg
    26461 gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc ctgcagagct
    26521 tcaacctgga agctccggtt gacgatcacg aacgtatcga gaaagttcag gaatacgttg
    26581 ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag cgcagccgtc
    26641 gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa gcgggcaaac
    26701 gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct atctgtgctg
    26761 aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac cgtgttgcag
    26821 cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa gtggttcgcg
    26881 aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc gaaaccgttg
    26941 cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa caggatgaag
    27001 ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt ccgccgctgc
    27061 agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc atgctgctgc
    27121 cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc gctgaacagc
    27181 tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc gaaccgcgcg
    27241 ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta gaaaaagttc
    27301 gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac ggtccgctgc
    27361 agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac tctccggttg
    27421 caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc acctacaaag
    27481 cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt atgcgcaaac
    27541 cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc atcgcgctga
    27601 ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt tgcgctgcgg
    27661 atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt tgcattaatc
    27721 ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct cactttgcat
    27781 acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc actgaatcca
    27841 aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt gttgctattt
    27901 taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag gaagtgcagt
    27961 atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca tttcgcattc
    28021 tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg acgggcgatg
    28081 aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg aaatacggga
    28141 cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc attgtgatgc
    28201 gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc gggaatgaaa
    28261 ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta tttggtatgg
    28321 gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg ctgggctatg
    28381 acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt gccagttcgt
    28441 ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg gtgctttctg
    28501 gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg atctttacca
    28561 tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat gagtccgacc
    28621 gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt ggtgactggc
    28681 tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg atcgttaatg
    28741 cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg attggcatca
    28801 tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca tcctttaccg
    28861 aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa gggattttgc
    28921 tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc ctcaacagca
    28981 ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg ttgctcttcc
    29041 aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcgtta actatgccgt
    29101 tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg ctggcgttcc
    29161 agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta atggcaacgc
    29221 tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc ctgcttggac
    29281 tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc taccactaaa
    29341 aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc tctacggctt
    29401 catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct ggagtgaact
    29461 catcgcagcg tgcggtgatt tcttccggcg tcagccagca aacttcactg acttcatctt
    29521 cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag acacggcaat
    29581 ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca atgcccaact
    29641 cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga actacaccgc
    29701 ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga cgctggacca
    29761 gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc agacactgtg
    29821 cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg acaatatcca
    29881 cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct ttttctggcg
    29941 ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc tgcgcaatga
    30001 tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg ccataactcg
    30061 ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa atctcccccc
    30121 gctgttctgc cactggcaaa tgggtatgac cgtacaccag cacatcgttc tggtttaaag
    30181 caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt tgtttttcca
    30241 gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga tccacttcgc
    30301 tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc cgttcagcga
    30361 ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg ccgtgattca acacgtcgcc
    30421 aaggatcacc agccactggg caccgctttg gacaaacaac tccacaacac gttccgtcgc
    30481 cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa
    30541 aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc ttcgccgaaa
    30601 gtgcaataaa acgctggaca gacgctcgct gccactggaa tgtcgcataa tccaccaggc
    30661 gttctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat
    30721 cagcaatgca ccattcacca aataaattcg gctgacccag tgctaacaaa tgttctgcca
    30781 tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct tttttcgccc
    30841 ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg ctgcgcagcc
    30901 aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc
    30961 aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact
    31021 caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg
    31081 gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac aggccttttt
    31141 cctgcaacgc cacccaggcg gataacacat aaggggagaa aaagtgggca tctgaccaaa
    31201 gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta
    31261 tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata
    31321 cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct atttcgcccc
    31381 gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg attatcgctt
    31441 gattaaggta gacctgggga aaggtggtca gtttcgtccg gaatttttgc gcatttcgcc
    31501 caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg aaccgttaag
    31561 cctctttgag tctggtgcca ttttgttgta tctggctgag aaaacaggac tctttttgag
    31621 tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg taggcggact
    31681 ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta
    31741 cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa acaagtggct
    31801 ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc
    31861 gtgggttaat gcctggactc gcctgcgaat tgacctcgca atgtatccgg cagtcaagaa
    31921 ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact
    31981 cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag
    32041 taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt
    32101 cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt
    32161 atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat
    32221 gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc
    32281 tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg
    32341 acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc
    32401 atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac atagtgatca
    32461 ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag ctgggccatc
    32521 aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc cctcgggtga
    32581 cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc
    32641 tggccggaga accgattgct gataaacgct ggactcacaa gcaaaaagag cgtctctgcc
    32701 aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgccagc gacacgccgc
    32761 cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg
    32821 ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc cgctgggaag
    32881 agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg
    32941 tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt cttggcctgg
    33001 gcgggccgat tggttccggt cggcagtatc tggcctggat tcatattgat gatatggtca
    33061 acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct
    33121 acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat cgcccggcta
    33181 ttttgcgcgt ccccacaacc gccattcggc tgttaatggg cgaatcttca gtactggtat
    33241 taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt
    33301 acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag caaacatccg
    33361 ccagttaact ccctgtgtta caggattagt ggctttgcgc gataagatcg tctggtgaaa
    33421 gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc tcctgagcaa
    33481 tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa
    33541 tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca ataaatgcac
    33601 ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt agctgtctgt
    33661 cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc
    33721 gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt tgcataatct
    33781 cgtcatccgg caccacggta atatccacca acggaaacgc tgatgaatat attttgcggg
    33841 ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc
    33901 aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc gcatcaagat
    33961 ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct
    34021 tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt ttcacagacc
    34081 acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttctagtt
    34141 taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat
    34201 tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag
    34261 ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc
    34321 acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt
    34381 tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa
    34441 tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt aaggaactgt
    34501 tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc ttcttctatt
    34561 ttcccctgat ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc catttcgtga
    34621 gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg taacacttcg
    34681 cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttccggttcc
    34741 atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag atgcaccgga
    34801 tatttcccct gcgcacgttc gtctatcccg actttcgcca gatacttcac cgcccgctcg
    34861 cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccaac
    34921 accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt
    34981 aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg caccagattg
    35041 atcgtctggc cattgaccac gatcgatcct tcactcggtt tttcgaggaa gttaatgcag
    35101 cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca
    35161 ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc catagcgttt gtgcaaatcg
    35221 ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg
    35281 ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa
    35341 atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc
    35401 gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag tagagtgcag
    35461 tatcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat
    35521 aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt caatttcccc
    35581 atgcggaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag
    35641 cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa ggaattcggt
    35701 tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact gaacatacag
    35761 cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact
    35821 ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat
    35881 aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg
    35941 tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag
    36001 cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag gtaaatcacg
    36061 ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag ctgcgtggct
    36121 ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc
    36181 cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga
    36241 aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc tttcggcact
    36301 gccataaacg cgccacggaa cgtttcggta aaataagcac cgtagataaa accgagagtg
    36361 ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc
    36421 atgtttagcg caatctgcaa accatagaaa atcagcagca tcaacaccaa gtccggcacg
    36481 cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc
    36541 ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct gatagccagc
    36601 tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct
    36661 gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg
    36721 ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg
    36781 ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc
    36841 gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat
    36901 ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg aggaaacctt
    36961 cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat
    37021 aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc cagtgttcat
    37081 taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact
    37141 cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg gcagcgtaca
    37201 gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc gatgacatga
    37261 tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcggg ttttcgacaa
    37321 acgtacattg cgtattgatg cgcttgcaca actccttcgc caactcgata tcgaaaccaa
    37381 ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc
    37441 ggatgttttg cggaattgca gcaagtgccg cagttgcgct ggagaaggcc agaaccagag
    37501 agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata
    37561 tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc
    37621 cccgttagac ttattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga
    37681 tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag cttcccagcg
    37741 acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggtg
    37801 ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcatcatct
    37861 ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca
    37921 gcaaaggcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca
    37981 tcttgtaacg cagcatccag acgccctgca gccagatcgg aatagaccaa atcctggttg
    38041 gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta agcttcctgg
    38101 gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt tggctgaatc
    38161 ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc ggagaaggca
    38221 atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc gatttttttc
    38281 gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cctaggtaca tttcacctgc
    38341 atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc acctttagca
    38401 tctttcgacg agaacggcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc
    38461 gccgcatagc tggaagccgc tgcggagaga ccgactaata aagacagagc gagaatcgac
    38521 ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg
    38581 tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt
    38641 taaatatgtt aataagacgt tgcattattg tcctgaagtt gaagatagca ggtatagcgg
    38701 ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt
    38761 gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag
    38821 atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct gatttatcac
    38881 atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc
    38941 gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac
    39001 gcagagtacc aacgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc
    39061 atcagtatag ctatggacaa tgccggaaag ggttttgatt gaacagggta aaatcaccat
    39121 ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg catcgtgcgt
    39181 gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg
    39241 gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg
    39301 taatacgcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt
    39361 cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg cgggagttgc
    39421 aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta tgcatttcga
    39481 gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt aacgtatcga
    39541 ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc
    39601 attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga tcgttcagat
    39661 cctggaaaat caccccgtca gcaccgatga tctggcgaat ttcatcaact tcgcgaccgt
    39721 gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt
    39781 ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa
    39841 tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg tttttatcgc
    39901 ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc agctgctggc
    39961 ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt
    40021 tgcccagaat acgtgcaatt tccagcgcga tatcacacga ggtttctggg atcgggatca
    40081 ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt ttcgtgccca
    40141 tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg cgggcaaagt
    40201 atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca
    40261 actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa
    40321 agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta cggttctcgt
    40381 caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg aaagcaacca
    40441 taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag cggtttgtgg
    40501 cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag ttgtccagct
    40561 cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt
    40621 tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa
    40681 gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc
    40741 cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc
    40801 gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg
    40861 catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat
    40921 cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc
    40981 tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc
    41041 tttgcagata atcaaaaaac catctgatga taaaactgaa ttgcgggatc agctgtgatt
    41101 tgctccagtc ttcacttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg
    41161 caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg
    41221 acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca ccaacgatca
    41281 gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc
    41341 ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc
    41401 atgtcaccag cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa
    41461 aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg
    41521 ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt
    41581 ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat
    41641 gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat ccgacctacg
    41701 gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca agactgtatt
    41761 aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca
    41821 gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg gtaattttac
    41881 cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga
    41941 caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca taggctttac
    42001 ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc gcaagtggtg
    42061 cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg accggtgcag
    42121 gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc gacggtgctg
    42181 cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct
    42241 gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc accagcggga
    42301 tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct ggaagtacaa
    42361 tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac ttacttgcca
    42421 ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa agaaccacac
    42481 accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg
    42541 ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc
    42601 ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca ggccagagtt
    42661 ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc gtttttcggt
    42721 agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc gacatcaaaa
    42781 ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc
    42841 ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc cgccagcgcc
    42901 gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt gccgtgagca
    42961 tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc ccgacgttgt
    43021 aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc cggctcaccg
    43081 acaattgccg gtttttcgct gcggaagatg cctgctttct cgcgaccaat actttcgcga
    43141 tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc gacatcggca
    43201 tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat caccacgtca
    43261 agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg
    43321 gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc cgattccagc
    43381 aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg cgaactgtag
    43441 acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca ggtggtgcct
    43501 ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac gccaagacgc
    43561 gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg caggttttcc
    43621 agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt gataatcatg
    43681 gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg ccctgccctt
    43741 atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac gccttcacgc
    43801 ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct cgccagtttc
    43861 aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat caggaattca
    43921 ctgcgctgga atctaggcgg cagtttttcg cgaacggtct gttcgataac acgcggaccg
    43981 gcaaagccga ttaacgcttt aggttcagcg atgttgagat cgcccagcat ggcgaaactt
    44041 gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc
    44101 tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat cagtgcttcc
    44161 tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc ttcctgcgcc
    44221 tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc gcccataaag
    44281 gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt catcaccacc
    44341 agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt atacttcttg
    44401 gagtcacgaa acttcagctc atctttcggc tcaagctcgc tacccagctc cacaaggctt
    44461 ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg atggtcacac
    44521 ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg accgcagcta
    44581 tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt
    44641 ttaattcgtt caatccagct cattagggac ctttctgtct gaacctagtt cgatgccagt
    44701 tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa tgttgcacat
    44761 taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga gttacttttt
    44821 attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg cccggcaaaa
    44881 tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc atcgggattg
    44941 taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg ccgatcacgt
    45001 tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac ggggcgaacg
    45061 ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt ttctcataaa
    45121 actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga ttactgaata
    45181 acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca atcgcggcga
    45241 tcagcatcag caccaccatc atatggacgt tgagatcgtt ggtttccagt gatgccaacg
    45301 ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cattaccacc agaccggttt
    45361 cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc gcgactagct
    45421 ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc aggtccatat
    45481 ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta cagcctgaaa
    45541 tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg cggttttgga
    45601 agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc ttttgccgtt
    45661 gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct ctccggctgg
    45721 ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg atgtacaaag
    45781 gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt aatatgcata
    45841 acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt gaaatcattc
    45901 tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa
    45961 tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat gatgtagcga
    46021 taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc tttaacccaa
    46081 cgcacggcga tgtcaccagg taaattcgca tttacgccca aggtccacgc cgcgtctttg
    46141 cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc tgcgtcagta
    46201 cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc cttttccagc
    46261 ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt actgccgtcg
    46321 tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga cattagtaca
    46381 gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa
    46441 cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca ttacgcacgc
    46501 agccaacaga aagatgcggc gtacccgaag catcccctac ctgagtcggg aattcgttct
    46561 cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct tctgccgcca
    46621 gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc ggtgcctgga
    46681 cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact tcgtcaacga
    46741 tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc atattgaacg
    46801 ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc aatttcgcac
    46861 tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa atcaggctgg
    46921 taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc gccagcagct
    46981 ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg gtcagtacaa
    47041 acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac agtccgctgc
    47101 tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg gtagcttctt
    47161 tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg gcatcctgca
    47221 cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg
    47281 cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg gcttcgccca
    47341 tagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg gtttactcca
    47401 gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag attaacgtgc
    47461 cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac tggcgtcatc
    47521 acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc gcagtttatc
    47581 gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc gcacatcata
    47641 caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggaccatgca gcgtaatgcg
    47701 gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat gcccaataaa
    47761 cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca gggtatagcc
    47821 tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattaa gttccggctc
    47881 gccttcccag acatccagca ccacgcttaa cttctggcct tcattcaggc aggtcagcag
    47941 cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc cgggcttcag
    48001 gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat ctttaaagag
    48061 tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg agcggaaatc
    48121 accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa tccctaacgc
    48181 ttccagtcgc acctgcaatc gacgtccaac gttaccaacg cccacgatcc ccaccgtacg
    48241 gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga aaacatattc
    48301 caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa
    48361 ccatgcttca tcgacatggt cggtccccgc agtggcagtt ccaacaaatt taatgggttt
    48421 tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg catccgcgtc
    48481 tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac ccaaacggct
    48541 aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt ttgtgttacc
    48601 tgtatgagac gagagttaac cggacaagtg tgccataatc tcgcggccag gcatacttgc
    48661 gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct cctcctaggc
    48721 agccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct ttatccagtc
    48781 agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg caacaacaaa
    48841 gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat tcaccgctgc
    48901 tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg gccgcgcaaa
    48961 aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt ctggggaata
    49021 atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg ctgaatcagc
    49081 tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag atggttattc
    49141 cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag cacaatgcgc
    49201 taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa cagatctggc
    49261 aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg aaactgttta
    49321 accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat acgccgacgc
    49381 tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg gcggcgttat
    49441 cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct tctgcccagg
    49501 ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat ccgcgtgata
    49561 tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat atggcaacgc
    49621 gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt
    49681 aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag atcgccccgg
    49741 caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga
    49801 cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg
    49861 aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa catccggctc
    49921 cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag agcagcaaca
    49981 agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca aaacggtccg
    50041 gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa
    50101 agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc ccgataaccg
    50161 caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc ccgacaacgc
    50221 tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg ccgttacagg
    50281 cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa ccgtaatggc
    50341 tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc gccattccgt
    50401 tccatgacat cactttgcca gagtgtttta gccctacgat gcctaacccc caggtcagag
    50461 cgcctgtcag tgactggctt tcaccaaacc caagaatcaa acgcccgatg accaacaggg
    50521 caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca
    50581 gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga tcggccagtc
    50641 gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg gcaatgccga
    50701 ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc ggcaacggca
    50761 accctacggt catgtaggtg agaaaaaccg caaaagcgat gcggaagagc gaaaaactgg
    50821 cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga atgcagcgca
    50881 aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg ttggcaggat
    50941 ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc tggaactggt
    51001 ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt tagtgccgga
    51061 ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga cctactccgg
    51121 cctactgcga attaatcttt cagcttgcgc attaccagcg tggcgttggt gccgccgaag
    51181 ccgaagctgt tagacataac tgtggtcagt tcgcgatcgg tcgtttcggt cacgatgttc
    51241 agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat aaagccgtgt
    51301 tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag tgagtgaccg
    51361 gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc acggatagct
    51421 gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag gtaatcgatt
    51481 ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc gccagacgga
    51541 gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc atagatgtaa
    51601 gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc gccgccagcg
    51661 ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg ggtgtcgttg
    51721 tatttagtag acagcgcacc catcgcgtcg aattcgcagg ccatttccca gcacagctct
    51781 tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc tactgcgtta
    51841 ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac gccatgaatt
    51901 ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt gaccacatac
    51961 gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa cacctggaaa
    52021 cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt ctggtaagct
    52081 tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc ataaatggat
    52141 gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc cagttttacg
    52201 ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa agtgatccct
    52261 gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat gctggaaaca
    52321 atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc gcacatagag
    52381 taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat cagccattta
    52441 atagagaaat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc ctgtaagacg
    52501 agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta atgctgaggg
    52561 tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg ggctggaaga
    52621 gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg agcatccaca
    52681 tcctctgttt gtggtagcag agagcggctt cggcaccgga ttaaacttcc tgacgctatg
    52741 gcaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac gcttacattt
    52801 cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc atcaacactg
    52861 gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc ccttgcccgg
    52921 ttgccatcgt ttattgttcg atgaaggccg cgtgacgctg gatttatggt ttggcgatat
    52981 taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg cctggtttct
    53041 ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt ttaacgccat
    53101 ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg gttttgtccg
    53161 ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg ggcgcaaacg
    53221 ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca cgccgtggtt
    53281 taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta ttgccagcgc
    53341 gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt gcgcggatga
    53401 agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt tattaagcaa
    53461 acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc gtcggtttta
    53521 cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt taggctggga
    53581 tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg cagaactggc
    53641 tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa attgcagcgg
    53701 cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta atgtgctgga
    53761 actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga atttatcccg
    53821 taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac acagcgtagt
    53881 ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc tcccggtgta
    53941 ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag agctgaagca
    54001 ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac atcattgtat
    54061 tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg atcagcagca
    54121 gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag aggttgatgt
    54181 cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc tgccaatggt
    54241 aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg cggagcaaaa
    54301 agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg ctttaggttc
    54361 tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga tgagcgacga
    54421 accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt tatgggtgcg
    54481 gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag tgtttgccgg
    54541 atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata agacgcgata
    54601 agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac gcctgctgaa
    54661 acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat tcaccggcac
    54721 caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg gagtggtcat
    54781 caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac ccactggcaa
    54841 acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga atgcgtgatt
    54901 caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt cagttgagcg
    54961 cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa ttttgtagtg
    55021 ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc atcacctgcg
    55081 catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc agattgtcgc
    55141 aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac gcctgcggct
    55201 tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc gggcaatacc
    55261 agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag tgcgaaatct
    55321 catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac ggtacttccg
    55381 catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta ttaaattcat
    55441 cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta ctgttcatta
    55501 gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta atttggcact
    55561 catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt tgctcaacac
    55621 caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa agcccgtcgc
    55681 ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg acgttgcgtt
    55741 gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagcg taacgaaggc
    55801 cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac cgacgcagcc
    55861 accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatatt cttcacccag
    55921 ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca aaatctgccg
    55981 taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc cgacaaaggt
    56041 catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa taaagtagat
    56101 agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg cgttagcggg
    56161 agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac cggcaatcga
    56221 gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca gcggagaaac
    56281 cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag cgcctggcag
    56341 gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg ttttggaggt
    56401 tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa aggttgatct
    56461 tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc gcgatgctgg
    56521 aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag gcaaagttgt
    56581 tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc ggcgagtttg
    56641 atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc gtctcgggaa
    56701 actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt tttcggcagt
    56761 tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc gttgaaacgc
    56821 ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag attgctcacc
    56881 tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata gcgacgctga
    56941 tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc gccgacaata
    57001 cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg ggttattttt
    57061 tgccacggcg tcgctgccag gctagcgcta ctggcaagca gagccagcag cgcaatcgcg
    57121 gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt ttgcgcccgc
    57181 tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat cggcactgca
    57241 cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc ttcgccaaag
    57301 cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc catatgagca
    57361 atgatttgct gcccgctgct gataccgccg agaatgccgc ccgcatggtt gctctggaaa
    57421 ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac gtcaaaaccg
    57481 tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc gatgtcggca
    57541 tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc aacaacggtg
    57601 actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc atctaacgcg
    57661 tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga ccagtctttg
    57721 atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat accaaatttc
    57781 tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt ttcgcgggcg
    57841 gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta ggtgtaatca
    57901 gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg ctggtcggta
    57961 ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac accggagagt
    58021 attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc agggcgacga
    58081 cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg aacaccatcg
    58141 acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg aaagagttgt
    58201 ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt ttacttaatc
    58261 tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga gcataaacac
    58321 accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat attgttccat
    58381 aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag caaggtaatc
    58441 tgccgcgtta ccgagaatgc gacacgtcag tttcaggccg tcggtgccag atgccaggcc
    58501 cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg cgtcgacata
    58561 cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga acagatcgga
    58621 acgaatcgga atgacattgt ggatcagacc gtgttcttcg atgttctgtt cggcaaccgc
    58681 cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg cataagcaca
    58741 ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg gttgcttgct
    58801 gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg gcaccagcac
    58861 gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca ggtaagccac
    58921 cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt tttcgctgga
    58981 ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga gcgatggtaa
    59041 caccagctgt acggcttcat cccactggtt atcggtgccg tgaccgtacc agatatttgc
    59101 cgcgctgaag cggcttaccg accagcgcaa catgtcctga atggtttgca gctcatttac
    59161 tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc aataatttcg
    59221 gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg agtgaggaaa
    59281 aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa aattagaaat
    59341 agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc tctgtttcgc
    59401 cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg accgcagcgt
    59461 aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga tgccagccat
    59521 tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa atatgttcgc
    59581 ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc accggaattg
    59641 tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg cgcgttgatt
    59701 gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca cgggaagcat
    59761 attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat ggcatttcac
    59821 caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga agtggaagag
    59881 tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc gtaaacgcct
    59941 tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt aggcctgata
    60001 agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga aaacgacacg
    60061 tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg tgtagtactt
    60121 tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca caaccgtaag
    60181 aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca tcattatcag
    60241 aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa agcgtggtgg
    60301 tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca taattttcac
    60361 tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta tgcactgtag
    60421 gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat gcgatccaag
    60481 ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca caacgaaaaa
    60541 ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca gtcagtacca
    60601 aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg cctttatacg
    60661 caacagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta acattgaaaa
    60721 cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc acactgtcaa
    60781 aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca acactcagaa
    60841 cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa atactcaaag
    60901 gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct ctttgcctgt
    60961 aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt gacgcctgtt
    61021 gtattgttga ctggcaccga tgtgcctgcc gcagtttcaa taccaatccc agcgccggat
    61081 gctgatgacg aggagtcaaa ggccagaaag ccggatagag cactatcttc tgttcctgtc
    61141 aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg aaacaccaat
    61201 ggcactcgct ctgactgccc aaggttcatt aaatcgctgg cggcaatggc aggaaaatgt
    61261 acttctgcca actggctacc attaatcact ggcgtacacg atttactgat taaattgccg
    61321 gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat caggctacta
    61381 ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg gcccaggcct
    61441 caaagtccgc ctctggcaga acgacaccac tcttttttac tggcacagcc gttaacaccg
    61501 gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc cctgcctgtt
    61561 tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca aaatcgcttg
    61621 gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc gtatcgcagg
    61681 taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca ataagcacat
    61741 cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc ggctgcacta
    61801 aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac agcaaaataa
    61861 gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa attcagctgc
    61921 cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa ctcccgagga
    61981 aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat cactactttt
    62041 ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac cgttaatcac
    62101 gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt tacaaacaag
    62161 cgagtagtca atgggacgtt tataatcaac cccattgatc ttattaatga atacattacc
    62221 aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa gcacccgtaa
    62281 attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac aacatatcag
    62341 taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc cggcctcagc
    62401 ctttgcggct ctgctcttca tc
//
LOCUS       DADLSB010000007        56275 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.007, whole genome shotgun sequence.
ACCESSION   DADLSB010000007 DADLSB010000000
VERSION     DADLSB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.007"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..350
                     /gene="cmtB"
                     /locus_tag="JN331_000431"
     CDS             <1..350
                     /gene="cmtB"
                     /locus_tag="JN331_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HAY7258664.1"
                     /translation="LDKNYISENYIQAIKDSTINNGPYYILAPGVAMPHARPECGALK
                     TGMSLTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTA
                     SSEKQLADIISRG"
     gene            378..1766
                     /locus_tag="JN331_000432"
     CDS             378..1766
                     /locus_tag="JN331_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAY7258665.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITCLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLPVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1781..3058
                     /locus_tag="JN331_000433"
     CDS             1781..3058
                     /locus_tag="JN331_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7258666.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3055..4020
                     /gene="yggF"
                     /locus_tag="JN331_000434"
     CDS             3055..4020
                     /gene="yggF"
                     /locus_tag="JN331_000434"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7258667.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQMANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4042..4551
                     /gene="fumE"
                     /locus_tag="JN331_000435"
     CDS             4042..4551
                     /gene="fumE"
                     /locus_tag="JN331_000435"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY7258668.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4548..5261
                     /locus_tag="JN331_000436"
     CDS             4548..5261
                     /locus_tag="JN331_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY7258669.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5233..5910)
                     /locus_tag="JN331_000437"
     CDS             complement(5233..5910)
                     /locus_tag="JN331_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7258670.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPPAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(5904..6581)
                     /locus_tag="JN331_000438"
     CDS             complement(5904..6581)
                     /locus_tag="JN331_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY7258671.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6569..6952)
                     /locus_tag="JN331_000439"
     CDS             complement(6569..6952)
                     /locus_tag="JN331_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY7258672.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(6968..7276)
                     /locus_tag="JN331_000440"
     CDS             complement(6968..7276)
                     /locus_tag="JN331_000440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258673.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWLHAITLWLLILAIVSTSQL
                     "
     gene            complement(7277..7855)
                     /locus_tag="JN331_000441"
     CDS             complement(7277..7855)
                     /locus_tag="JN331_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY7258674.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7878..8309)
                     /locus_tag="JN331_000442"
     CDS             complement(7878..8309)
                     /locus_tag="JN331_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7258675.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8681..9700
                     /gene="epd"
                     /locus_tag="JN331_000443"
     CDS             8681..9700
                     /gene="epd"
                     /locus_tag="JN331_000443"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY7258676.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9750..10913
                     /gene="pgk"
                     /locus_tag="JN331_000444"
     CDS             9750..10913
                     /gene="pgk"
                     /locus_tag="JN331_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY7258677.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11128..12207
                     /gene="fbaA"
                     /locus_tag="JN331_000445"
     CDS             11128..12207
                     /gene="fbaA"
                     /locus_tag="JN331_000445"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY7258678.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12398..13258
                     /locus_tag="JN331_000446"
     CDS             12398..13258
                     /locus_tag="JN331_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY7258679.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13397..14032
                     /gene="argO"
                     /locus_tag="JN331_000447"
     CDS             13397..14032
                     /gene="argO"
                     /locus_tag="JN331_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY7258680.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14125..14865
                     /locus_tag="JN331_000448"
     CDS             14125..14865
                     /locus_tag="JN331_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY7258681.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15032..15928
                     /locus_tag="JN331_000449"
     CDS             15032..15928
                     /locus_tag="JN331_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7258682.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     LTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(15925..17403)
                     /gene="scpC"
                     /locus_tag="JN331_000450"
     CDS             complement(15925..17403)
                     /gene="scpC"
                     /locus_tag="JN331_000450"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY7258683.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYNNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17427..18212)
                     /gene="scpB"
                     /locus_tag="JN331_000451"
     CDS             complement(17427..18212)
                     /gene="scpB"
                     /locus_tag="JN331_000451"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY7258684.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDNLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18223..19218)
                     /gene="meaB"
                     /locus_tag="JN331_000452"
     CDS             complement(18223..19218)
                     /gene="meaB"
                     /locus_tag="JN331_000452"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY7258685.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMMLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDSDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19211..21350)
                     /gene="scpA"
                     /locus_tag="JN331_000453"
                     /pseudo
     CDS             complement(19211..21350)
                     /gene="scpA"
                     /locus_tag="JN331_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(21554..22447)
                     /locus_tag="JN331_000454"
     CDS             complement(21554..22447)
                     /locus_tag="JN331_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY7258686.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22830..23489
                     /gene="rpiA"
                     /locus_tag="JN331_000455"
     CDS             22830..23489
                     /gene="rpiA"
                     /locus_tag="JN331_000455"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY7258687.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            23745..24977
                     /locus_tag="JN331_000456"
     CDS             23745..24977
                     /locus_tag="JN331_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY7258688.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25206..25265
                     /gene="ibsC"
                     /locus_tag="JN331_000457"
     CDS             25206..25265
                     /gene="ibsC"
                     /locus_tag="JN331_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY7258689.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25598..25657
                     /locus_tag="JN331_000458"
     CDS             25598..25657
                     /locus_tag="JN331_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7258690.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(25758..26360)
                     /locus_tag="JN331_000459"
     CDS             complement(25758..26360)
                     /locus_tag="JN331_000459"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY7258691.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26381..26564)
                     /gene="ssrS"
                     /locus_tag="JN331_000460"
     ncRNA           complement(26381..26564)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JN331_000460"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26606..26935)
                     /gene="zapA"
                     /locus_tag="JN331_000461"
     CDS             complement(26606..26935)
                     /gene="zapA"
                     /locus_tag="JN331_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY7258692.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27103..27681
                     /gene="ygfB"
                     /locus_tag="JN331_000462"
     CDS             27103..27681
                     /gene="ygfB"
                     /locus_tag="JN331_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY7258693.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            27707..29032
                     /gene="pepP"
                     /locus_tag="JN331_000463"
     CDS             27707..29032
                     /gene="pepP"
                     /locus_tag="JN331_000463"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY7258694.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQDEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29029..30207
                     /gene="ubiH"
                     /locus_tag="JN331_000464"
                     /gene_synonym="visB"
     CDS             29029..30207
                     /gene="ubiH"
                     /locus_tag="JN331_000464"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY7258695.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLVAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30230..31432
                     /gene="ubiI"
                     /locus_tag="JN331_000465"
                     /gene_synonym="visC"
     CDS             30230..31432
                     /gene="ubiI"
                     /locus_tag="JN331_000465"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY7258696.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGIQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31506..31589)
                     /locus_tag="JN331_000466"
     CDS             complement(31506..31589)
                     /locus_tag="JN331_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258697.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            31880..32974
                     /gene="gcvT"
                     /locus_tag="JN331_000467"
     CDS             31880..32974
                     /gene="gcvT"
                     /locus_tag="JN331_000467"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY7258698.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            32998..33387
                     /gene="gcvH"
                     /locus_tag="JN331_000468"
     CDS             32998..33387
                     /gene="gcvH"
                     /locus_tag="JN331_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY7258699.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33506..36379
                     /gene="gcvP"
                     /locus_tag="JN331_000469"
     CDS             33506..36379
                     /gene="gcvP"
                     /locus_tag="JN331_000469"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY7258700.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDSHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            36646..37389
                     /locus_tag="JN331_000470"
     CDS             36646..37389
                     /locus_tag="JN331_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7258701.1"
                     /translation="MAIALVTGGSCGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37446..38879)
                     /locus_tag="JN331_000471"
     CDS             complement(37446..38879)
                     /locus_tag="JN331_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7258702.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            38924..39235
                     /locus_tag="JN331_000472"
     CDS             38924..39235
                     /locus_tag="JN331_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY7258703.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            39399..40058
                     /locus_tag="JN331_000473"
     CDS             39399..40058
                     /locus_tag="JN331_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY7258704.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40254..41234)
                     /gene="ygfZ"
                     /locus_tag="JN331_000474"
     CDS             complement(40254..41234)
                     /gene="ygfZ"
                     /locus_tag="JN331_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY7258705.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTALAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41477..41743
                     /gene="sdhE"
                     /locus_tag="JN331_000475"
     CDS             41477..41743
                     /gene="sdhE"
                     /locus_tag="JN331_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY7258706.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            41724..42131
                     /locus_tag="JN331_000476"
     CDS             41724..42131
                     /locus_tag="JN331_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258707.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSCMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42171..42692)
                     /gene="fldB"
                     /locus_tag="JN331_000477"
     CDS             complement(42171..42692)
                     /gene="fldB"
                     /locus_tag="JN331_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY7258708.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            42804..43700
                     /gene="xerD"
                     /locus_tag="JN331_000478"
     CDS             42804..43700
                     /gene="xerD"
                     /locus_tag="JN331_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY7258709.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDLPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            43725..44435
                     /gene="dsbC"
                     /locus_tag="JN331_000479"
     CDS             43725..44435
                     /gene="dsbC"
                     /locus_tag="JN331_000479"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY7258710.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            44441..46174
                     /gene="recJ"
                     /locus_tag="JN331_000480"
     CDS             44441..46174
                     /gene="recJ"
                     /locus_tag="JN331_000480"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY7258711.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46265..47363
                     /gene="prfB"
                     /locus_tag="JN331_000481"
     CDS             join(46265..46339,46341..47363)
                     /gene="prfB"
                     /locus_tag="JN331_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY7258712.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47373..48890
                     /gene="lysS"
                     /locus_tag="JN331_000482"
     CDS             47373..48890
                     /gene="lysS"
                     /locus_tag="JN331_000482"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY7258713.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(48933..49481)
                     /gene="idi"
                     /locus_tag="JN331_000483"
     CDS             complement(48933..49481)
                     /gene="idi"
                     /locus_tag="JN331_000483"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY7258714.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            49536..49607
                     /locus_tag="JN331_000484"
     CDS             49536..49607
                     /locus_tag="JN331_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258715.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(49604..49729)
                     /locus_tag="JN331_000485"
     CDS             complement(49604..49729)
                     /locus_tag="JN331_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258716.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(49731..51179)
                     /locus_tag="JN331_000486"
     CDS             complement(49731..51179)
                     /locus_tag="JN331_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY7258717.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            51615..53534
                     /gene="ygfT"
                     /locus_tag="JN331_000487"
     CDS             51615..53534
                     /gene="ygfT"
                     /locus_tag="JN331_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HAY7258718.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQRIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFERHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEQGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            53534..54022
                     /locus_tag="JN331_000488"
     CDS             53534..54022
                     /locus_tag="JN331_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7258719.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDIISAPVMCHQCENAPCVGACPVQALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRLRQQRIVASGEN
                     PL"
     gene            complement(54058..55425)
                     /locus_tag="JN331_000489"
     CDS             complement(54058..55425)
                     /locus_tag="JN331_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7258720.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(55461..56036)
                     /locus_tag="JN331_000490"
     CDS             complement(55461..56036)
                     /locus_tag="JN331_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAY7258721.1"
                     /translation="MCPYYVGRINLDVYHQYGLTGKNCVFAHCVHLEEKEWDRLSETK
                     SSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQ
                     GYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPSKEADFVVMEPTATPLQQLRYDNSVS
                     LVDKLFVMMTLGDDRSIYRTYVDGRLGYERN"
     gene            complement(56039..>56275)
                     /locus_tag="JN331_000491"
     CDS             complement(56039..>56275)
                     /locus_tag="JN331_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7258722.1"
                     /translation="RHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMR
                     ATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
BASE COUNT        13635 a        14044 c        15132 g        13464 t
ORIGIN      
        1 tgctggataa aaactatatc agcgagaatt acattcaggc aattaaagat tccaccatta
       61 acaatggccc ttattatatt ctcgcaccag gcgtggcaat gcctcatgcg cgaccggaat
      121 gtggggcgct taaaaccggg atgtctttga cattacttga acaaggtgtt tattttccgg
      181 ggaatgacga accaattaaa ttactcatcg gactctctgc tgccgatgcc gattcgcaca
      241 ttggcgctat tcaggcgtta agtgaattac tgtgcgaaga agaaatactc gaacaactct
      301 taacagcatc atcagaaaaa caattagcgg acattatcag ccgcggataa ttactctctc
      361 ctctacagga taatattatg gaaaacaagt ctgctcgtgc aaaggtccag gcttttgggg
      421 gatttttgac ggcaatggtc atccccaata taggtgcttt tattgcctgg ggcttcataa
      481 ctgcgttatt tattcccacc ggttggctgc ctaacgaaca tttcgccaaa attgtcggcc
      541 cgatgattac ctgtttattg cccgtgatga ttggttctac aggtggtcat ctggtcggcg
      601 gtaaacgcgg ggcggtcatg ggcggaatag gtactattgg tgtgatcgtt ggcgcagaga
      661 tcccgatgtt ccttggctca atgatcatgg gaccgctcgg tgggttggtc ataaaatatg
      721 tcgataaggc actggaaaaa cgcatacctg ccggttttga gatggttatc aataacttct
      781 cattaggtat cgcggggatg ctcctttgtc tgctgggttt tgaagtgatc ggcccggcgg
      841 tgttaattgc caatactttc gtcaaagagt gtattgaggc gctggtacat gcgggttatc
      901 tgcctctgct gccagtcatc aatgaaccgg cgaaagtgct tttccttaat aatgcgatcg
      961 atcagggcgt ctattatccg ctgggaatgc aacaggcttc ggttaacggt aaatccatct
     1021 tctttatggt ggcctctaac ccaggtccgg gcctggggct gctgctggcg tttaccttgt
     1081 ttggtaaagg gatgagtaaa cgttctgcgc ccggggcgat gattattcac ttcctcggtg
     1141 ggatccacga actgtatttc ccatatgtgc tgatgaagcc gctgactatt attgccatga
     1201 ttgcgggcgg tatgtctggt acctggatgt ttaacctact ggacggtggt ctggtggctg
     1261 gcccaagtcc ggggtctatc tttgcgtacc tggcactgac gccgaaaggc tcgttcctgg
     1321 cgacaattgc cggtgttacg gtaggtaccc tggtgtcctt tgctattact tcgctgatac
     1381 tgaagatgga aaaaacggtg gaaacggaga gcgaagatga gtttgctcag tcagccaatg
     1441 cggttaaggc gatgaaacaa gagggtgcat tctcgttaag cagggttaag cgtatcgcct
     1501 ttgtttgcga tgcggggatg ggctccagtg cgatgggcgc gaccaccttc cgtaaacgcc
     1561 tggaaaaagc ggggctggca atagaagtaa aacattacgc gatagaaaac gtgcctgcgg
     1621 atgccgatat cgtcgtcact cacgccagtc tggaagggcg cgtgaaacgt gtgacggata
     1681 aaccgctgat attgattaat aactatattg gcgatccaaa actcgacact ttatttaatc
     1741 aattaaccgc cgaacataaa cactgattac agaggtaaaa atgaaaacca aagttgctgc
     1801 tatttatggc aagcgggatg tccgtctgcg cgaatttgaa ctgccagaaa ttaccgataa
     1861 tgaactgtta gtgagtgtaa tttctgacag cgtctgttta tcgacctgga aagcggcgtt
     1921 actcggtagt gaacataaac gcgtacccga cgatttagaa aatcatccgg tcattaccgg
     1981 gcatgaatgt gccggggtta ttgtcgaagt gggtaaaaat ctcactggca aatataaaaa
     2041 aggccagcgt tttgtattgc aaccggcgat ggggttacca agcggatatt cagcgggcta
     2101 cagctacgaa tattttggcg gcaatgccac ttatatgatt attcccgaaa tcgccattaa
     2161 tttgggctgc gtattaccgt atcacggctc ttattttgct gcggcgtcgc tggcagagcc
     2221 tatgtgctgc attattggtg cttatcatgc caattatcac accacgcaat atgtttatga
     2281 gcatcgcatg ggcgtcaaac ctggcggcaa tattgcactg ctggcgtgtg cgggtccgat
     2341 gggcattggc gctatcgatt acgccattaa cggtggcata caaccgtcac gggtggtggt
     2401 ggtcgatatc gacgacaaac gcctggcgca ggtgcagaag ctgctgccgg tggatctggc
     2461 ggccagtaaa ggcattgagc tggtgtatgt gaataccaaa gggatgagcg atcctatcca
     2521 gatgctgcgg gcgctgacag gagatgtcgg gttcgatgac atttttgttt atgcggcggt
     2581 gcctgctgtc gttgagatgg ctgacgaatt actggcggaa gatggctgtc tgaacttctt
     2641 tgccgggccg acggataaaa acttcaaagt gccgtttaat ttctacaacg tccattacaa
     2701 cagcacgcac gtagtcggta catccggcgg ttcaacggac gacatgaaag aggcgattgc
     2761 ccttagcgcc acaggacagt tacagccgtc gtttatggtg acccatattg gtggcctgga
     2821 tgcggtgcca gaaaccgtgc tcaatctgcc ggatatccct ggcggtaaaa aactcattta
     2881 taacggcgtg accatgccgc tcactgccat tgccgatttt gccgaaaaag gcaaaaccga
     2941 tccgctgttt aaagagttgg cgcggctggt tgaggaaacg cacggcatct ggaatgaaca
     3001 ggccgagaaa tatctgctgg cacaatttgg cgttgatatc ggggaggccg cgcaatgatg
     3061 tccctggcgt ggccattatt tcgcgttacg gaacaggcag cgttggctgc ctggccgcaa
     3121 accggatgtg gcgacaaaaa taaaattgat ggcctggcgg tcaccgcgat gcgccaggca
     3181 ttaaacgacg tcgcttttcg tggacgagtg gttatcggcg aaggagaaat tgaccatgcg
     3241 ccgatgctgt ggattggcga agaggtgggc aaaggcgatg ggccagaagt cgatatcgcg
     3301 gttgatccca ttgaaggcac gcggatggtg gcgatggggc agagcaatgc gctggcggtc
     3361 atggctttcg ctcctcgaga tagcctgtta catgcgcccg atatgtatat gaaaaagctg
     3421 gttgttaatc gactggctgc cggggcgatt gatctgtctt tgcctctggc ggacaacttg
     3481 cgtaacgtgg cgcgggcgtt aggtaaaccg ctggacaagc tgcgcatggt tacgctggat
     3541 aaaccgcgtc tgagtgccgc gattgaagaa gcaacacagc tgggcgtgaa ggtttttgcc
     3601 ctgccggatg gcgatgtcgc cgccagcgtg ctgacctgct ggcaggataa tccttacgat
     3661 gtgatgtaca ccatcggcgg tgcgccagaa ggcgtgattt ctgcctgtgc cgttaaagcg
     3721 ttaggcgggg atatgcaggc cgagctgatt gacttttgtc aggctaaagg ggattacacg
     3781 gaaaatcggc agattgcaga gcaggagcgc aagcgttgta aggcaatggg cgttgacgtt
     3841 aaccgtgtgt actcgctcga tgaactggtg agggggaacg atatcctctt tagcgctacg
     3901 ggcgtgacgg gcggcgagtt ggtgaacggt atccaacaga tggcgaatgg ggtgcggacg
     3961 cagacattac tgatcggcgg cgcggaccaa acgtgtaata taatagactc cctgcattga
     4021 tggtgatcta tcggaagacc aatggcgacg ctgacagaag atgatgtgct tgagcaactg
     4081 gatgcacagg acaatttatt ttcatttatg aaaactgcgc attctatttt gctccagggg
     4141 atacgccagt ttctgccgtc gctgtttgtc gataacgatg aagagatcgt cgaatatgca
     4201 gtgaagccgt tactcgccca aagcggcccg cttgacgata ttgatgttgc gctgcgtttg
     4261 atttatgcgc tggggaaaat ggataaatgg ctgtatgccg atatcacgca cttttcccag
     4321 tactggcatt acctgaacga acaggatgaa acgcccggat ttgccgatga tataacttgg
     4381 gattttatca gcaatgtcaa cagtataact cgcaatgcga cgctctacga tgcgttaaaa
     4441 gcgatgaagt tcgccgattt cgccgtctgg tcagaggcgc gttttagcgg aatggtcaaa
     4501 acggcgctga cgctggcagt aacgaccacc ttaaaggaat taacgccgtg aaaattgaat
     4561 taacggtgaa tgggctgaac gtgcaagcgc agtaccatga tgaagagata gaacgcgtgc
     4621 ataaaccgtt gctgcgtatg ctggcggcct tacagacggt aaatccgcag cggcgtacgg
     4681 tggtttttct ttgcgctccg ccggggacgg gtaaatctac cctaaccacc ttctgggaat
     4741 atctcgcgca acaagacccg gagctgcccg ctattcagac gctcccgatg gatggttttc
     4801 accattacaa tagctggctg gacgcgcatc aattgcgccc cttcaaaggc gcaccagaga
     4861 catttgacgt tgcgaaactg gcggaaaatc tgcgccaggt ggtggaaggg gattgtacgt
     4921 ggccgcagta cgatcgacaa aagcatgatc ctgttgaaga tgcgttgcac gttaccgcac
     4981 cactcgtcat cgtcgaagga aactggttgt tgctggacga cgagaagtgg tgccagcttg
     5041 cgcaattttg tgatttctcg atctttatca acgcgccagc tactgcgttg cgggagcgtc
     5101 tggtggggcg caagctggcg ggaggggtat cgctggcaga tgcagaagcc ttttatgacc
     5161 gtaccgacgg gccgaatgtt cgccgggtgc tggaagagag tctgcctgca aatttgacct
     5221 taatgatgac cgctacgggt gaatatcgtc tggtggatta acgttcctga ttacaccatc
     5281 ttccagtcgc accacacgag agaaatgacg ccgggtaaat gcggcgtcat gactgattgc
     5341 taccacgctc gtgcctcgct ggcggcactt ctccagccag ctttcaaaca cgctcaacca
     5401 attttcgtca aaatcccgac tcggctcgtc cagcagtaat agtggcggag agagtgcttc
     5461 caggcaggcg acggcgacca tccgtcgctg cgcactatgc agatccaacg ggtgcgagtt
     5521 ggcagtgtct gctaactgac aacattgcag ggcagcatgg gtacgttgag tgatttcatc
     5581 cgccgggcat ttttgcagtt tcaaaccaaa tgcgatctcg tcagcgacgg tgctgtgaaa
     5641 aagttggttt tccgcttcct gaaacagtac gccaaccttt gccgcgcgct ggcggttttt
     5701 caggtttttc atcgcctgtt gttgcagcat aaccgtaccg gctgggggcg taagaagacc
     5761 tgccattact cgtaacagcg tcgatttccc tgcgccattg tcaccggtca gcgccaacca
     5821 ttcaccttgt ttgagttgca gagaaatgtc gcaaagacaa tccgttgccg cgccaggcca
     5881 gcgataagat atcttattta acgttaacat agtggctgga gcgctccgtt ttgcaattgc
     5941 cacgcttgct ggcagtagtt caggaaaggt gtgcgatgac gttcaaaaag aataatcagc
     6001 gaaccacgtt caaacgccca gtgttgtaag cgctgcaaca gcatttcgct ggcctgcggc
     6061 gtcaggcggc taaaggcttc atcaagaatt aagagctttg gctgcatcgc gatggcgcag
     6121 gcgatgacca cgcgctgtgt ctctccaccg gagagtgttg ccgggtgacg atggcgcaac
     6181 ggctggcact cggtcagagt cagggcggca tcaatccgcg ccataatctc tgcttcagca
     6241 agacacagat tttctggccc aaaggcaact tcttcttcca cgctaaaggt acagccggag
     6301 agctgcaaat agggcgattg ctggacgagc tggatggtgg cggattgttc attaagcggg
     6361 agatgcccaa tgggcgtacc gaggagcgtc cccgtgcctg ttatctctct tggcagaaag
     6421 tccggatacc agcccgccat cagctgtgcc agcgtacttt tgccactgcc gttgtcacca
     6481 aagatggcga ccatccccgg tttgacatag tgaaagtcat aacagaacgg aggatgcgtt
     6541 gagtgtgtcg ggcaataacg gaactgttct aacgtaacca tatccacgct ccgaattctg
     6601 tcagcatcag caagatcatg gtgtaacgcg ctacgcgttg tagggtgcta tcggctggtg
     6661 cccacaacgt tgttcgtcgg ttgtggatcc gaaatgcccg catatccagc gccgcaccac
     6721 ggacagccag atcgttaagc gcattgtgtg ttagcggaat aattaacgcg ggcatagcac
     6781 gtaaacgctg ataccagccc tcatccagcg gcacgccgcg ggcacgctgc gcttcatgaa
     6841 taatcgctaa ttgccgtttt aactgctcaa cgaccaataa cggcccggca aacagataag
     6901 cgacgcctgg cggcaggcga gaagcaaaca gagcgcgaat aaaacattgc acggggacgt
     6961 actgcattca caactgtgag gttgaaacaa tcgccagaat cagcaaccag agcgtaatag
     7021 catgcaacca ccgttctggc gagcgtggcg tgccgcttag ccattctggt agccagccac
     7081 cgtgtaccag ccacagcccg gctcccagcg agaacatcag ccagacgaca tattttgccc
     7141 gccgccgcgt ggcctttagc gcaataaggc agaaaaaggt cgccgcgcta tagatcggca
     7201 aaatggtctg cgcgggcagg ataagcgtgg tgcaggccgc caatgcccac agtgttaatg
     7261 aggtaaatgg atgcatttag cgcacggcag ccattgccgg aaaatggcgc gtggtgcgaa
     7321 tcggcagctg gcgcagtaaa agccagacaa tcaccgctgt aagaatttta tcgaccaggt
     7381 tcgcgcctat caccgtaatc gccaccgact ccaccaggtt ttgccccatc gagtgcatcc
     7441 aggcaacgaa caaatcggct ccgctaccgg tgacaccgcc gaacagcgca gtacgcagcg
     7501 gtacagcgac gaccgttacc gcaagcgtaa taatcacgcc gctgacaacg acttttggca
     7561 gggtgcgaaa ccaacccgct cgcgccagcc aaccggcgac caggccgatg accatcgcga
     7621 cgggtgcaaa tgcggcggcg atcgggtcgg tcagcaatcc ccagagtaaa ttggtcagta
     7681 agccggtcag cattccgatc accggaccaa gcagtaccgc gctaataagt gtgccgatgg
     7741 agtcgagaaa tataggtaat ttgaccatgc tggccagttg cccgccgatc atattgatgg
     7801 cgatagatat aacgatcaac accagtgcct gactggaaaa gtggctacgc gccataagaa
     7861 aacctctttg aaagtagtta tttaacgggg gaaatatgca actcgtcgta accagaaagc
     7921 gcgcaaggtt ggcggctaaa cgccagttcc tcaaggctac cgatcaccag ttcaagcgtg
     7981 gtccgcacgg tacagccggg catcatatga ccgccaagca tcgtgccgtg tggatcggaa
     8041 acgcagagat gaagatgctc accactctgt tccagcgtgc cgtttagtga gatcacttcg
     8101 aatgtaccgt tcaggagcgt ggtgccttct tgcccggcat agcgcaaggc gacgtcagtc
     8161 aggctaccgg tacagcctgc aatccaggcg gcatgaagtt gatgttgctg tgcaaaagcg
     8221 tgtagctggg aaaaaacttc ctgcccgggg aggaggcgca gtgcgtaaaa gcgtgcggag
     8281 gaagaatatg ggtagggcaa tgtcatcatt ggagtactcc gaaactcgga cgtattgcgg
     8341 ataatatcga cgctgcatca gtcatattct gatataagtc agggtttgtg acggcacaac
     8401 gaaaagattt tgctacgctt attcgtgatt ctgcaaagtg gaataaattc cacaggggcg
     8461 gggctgaaaa cgtgacgtaa gtcagataat tgctttcttt gactggacaa tcattccttt
     8521 tattccacgt ttcgcttatc ctagctgaag cgtttcagtc gattaaatgt tcgacaatta
     8581 accaatcagt cgcagtttgc gacaggtaag gtttccccgg acgatttgct ggattactct
     8641 gtctggcaaa tttgctcagg gaaaaccttg caggagatct atgaccgtac gcgtagcgat
     8701 aaatggcttc ggtcgcatcg ggcgtaatgt ggttcgtgct ttgtatgaat ccggacgccg
     8761 ggcggaaatt accgtggtgg caatcaacga actggcggat gctgcgggca tggcgcattt
     8821 gttgaaatat gacaccagcc atggccgttt tgcatgggaa gtacgacagg aacgcgatca
     8881 actttttgtt ggtgatgacg ccatccgcgt attgcatgaa cgttcactgc aatcgctccc
     8941 ctggcgtgaa cttggcgttg atgtagtcct cgactgcacc ggcgtatatg gctcccgcga
     9001 gcatggcgaa gcgcatattg ccgccggggc caaaaaagtg ctcttttcac atcctggcag
     9061 taacgatctc gacgcgaccg ttgtttacgg cgtcaatcag gatcaacttc gtgcggaaca
     9121 ccgcatcgtt tctaacgctt cctgtaccac gaattgcata attcccgtca tcaaattgtt
     9181 agatgatgcg tacggtattg agtccggcac tgtgaccaca attcactccg ccatgcacga
     9241 tcaacaggtt attgatgcat accatcctga cctgcgtcgc acccgggcag ccagccagtc
     9301 gatcattccg gtcgatacta aactggccgc tggtatcaca cgattttttc cgcaatttaa
     9361 cgatcgcttt gaagcgattg cggtacgtgt gccaaccata aatgtgacgg caatcgattt
     9421 aagcgtgacg gtgaagaaac ctgtaaaagc caatgaagtc aacctgttgc tgcaaaaagc
     9481 agcacaaggt gcatttcatg gtatagttga ctatacggaa ttgccgttgg tctctgtaga
     9541 ttttaaccac gatccgcaca gtgccattgt cgatggcacc caaacccggg tcagtggcgc
     9601 acacctgatc aaaacgttgg tctggtgcga taacgaatgg ggctttgcta accgaatgct
     9661 cgacacgacg ttagctatgg ctactgttgc tttcaggtaa gacgcaagca gcgtctgcaa
     9721 aacttttaga atcaacgaga ggattcacca tgtctgtaat taagatgacc gatctggatc
     9781 ttgctgggaa acgtgtattt atccgtgcgg atctgaacgt accagtaaaa gacgggaaag
     9841 taaccagcga cgcgcgtatc cgtgcttctc tgccgaccat cgaactggcc ctgaaacaag
     9901 gcgcaaaagt gatggtaact tcccacctgg gtcgtcctac cgaaggcgag tacaacgaag
     9961 aattctctct gctgccggtt gttaactacc tgaaagacaa actgtctaac ccggttcgtc
    10021 tggttaaaga ttacctcgac ggcgttgacg ttgctgaagg tgaactggtt gttctggaaa
    10081 acgttcgctt caacaaaggc gagaagaaag acgacgaaac cctgtccaaa aaatacgctg
    10141 cactgtgtga cgtgttcgta atggacgcat tcggtactgc tcaccgcgcg caggcttcta
    10201 ctcacggtat cggtaaattc gctgacgttg cgtgcgcagg cccgctgctg gcagctgaac
    10261 tggacgcgct gggtaaagca ctgaaagaac ctgctcgccc gatggtggct atcgttggtg
    10321 gttctaaagt atctaccaaa ctgaccgttc tggactccct gtctaaaatc gctgaccagc
    10381 tgattgttgg tggtggtatc gctaacacct ttatcgcggc acaaggccac gatgtgggta
    10441 aatccctgta cgaagctgac ctggttgacg aagctaaacg tctgctgacc acctgcaaca
    10501 tcccggttcc gtctgatgtt cgcgtagcaa ccgagttctc tgaaactgca ccggctaccc
    10561 tgaaatctgt taacgatgtg aaagctgacg agcagatcct ggatatcggt gatgcttccg
    10621 ctcaggaact ggctgaaatc ctgaagaatg cgaaaaccat tctgtggaac ggtccggttg
    10681 gcgtgttcga attcccgaac ttccgcaaag gtactgaaat cgtggctaat gctatcgcag
    10741 acagcgaagc gttctccatc gctggcggcg gcgacactct ggcagcaatc gacctgttcg
    10801 gcattgctga caaaatctcc tacatctcca ctggcggcgg cgcattcctc gaattcgtgg
    10861 aaggtaaagt actgcctgca gtagcgatgc tcgaagagcg cgctaagaag taaaaaatca
    10921 cagggcaggg aaacctgccc ttgtttcagc gcgcttttag agcacgcatt ttttcatact
    10981 ctaaataatt cgagttgcag gaaggcgaca agagagtgaa tccctaggag cttacataag
    11041 taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta ttatgagtat
    11101 aaggcccgac gatacaggac aagagacatg tctaagattt ttgatttcgt aaaacctggc
    11161 gtaatcactg gtgatgacgt acagaaagtt ttccaggtag caaaagaaaa caacttcgca
    11221 ctgccagcag taaactgcgt cggtactgac tccatcaacg ccgtactgga aactgctgct
    11281 aaagttaaag cgccggttat cgttcagttc tccaacggtg gtgcttcctt tatcgctggt
    11341 aaaggcgtga aatctgacgt tccgcagggt gctgctatcc tgggcgcgat ctctggtgcg
    11401 catcacgttc accagatggc tgaacattat ggtgttccgg ttatcctgca cactgaccac
    11461 tgcgcgaaga aactgctgcc gtggatcgac ggtctgctgg acgcgggtga aaaacacttc
    11521 gcagctaccg gtaagccgct gttctcttct cacatgatcg acctgtctga agaatctctg
    11581 caagagaaca tcgaaatctg ctctaaatac ctggagcgta tgtccaaaat cggcatgact
    11641 ctggaaatcg aactgggttg caccggtggt gaagaagacg gcgtggacaa cagccacatg
    11701 gacgcttctg cactgtacac ccagccggaa gacgttgatt acgcatacac cgagctgagc
    11761 aaaatcagcc cgcgtttcac catcgctgcg tccttcggta acgtacacgg tgtttacaag
    11821 ccgggtaacg tggttctgac tccgaccatc ctgcgtgatt ctcaggaata tgtttctaag
    11881 aaacacaacc tgccgcacaa cagcctgaac ttcgtattcc acggtggttc cggttctact
    11941 gctcaggaaa tcaaagactc cgtaagctac ggcgtagtga aaatgaatat cgataccgat
    12001 acccaatggg caacctggga aggcgttctg aactactaca aagcgaacga agcttatctg
    12061 cagggtcagc tgggtaaccc gaaaggcgaa gatcagccga acaagaaata ctacgatccg
    12121 cgcgtatggc tgcgtgccgg tcagacttcg atgatcgctc gtctggaaaa agcattccag
    12181 gaactgaacg cgatcgacgt tctgtaagtc attcctttct gcttatctca aggcccgctc
    12241 tgcgggtctt tttttcgcca aaagcaaggt gattcagatg agaaatctgt gatctatttg
    12301 gcaaaattat gctttattgt ttacccttgt cagactgccc gtcataaggc ggcggagtgt
    12361 atttctccat tttgagtcag ttgaaaagga atattgaatg gaagatttga atgttgtcga
    12421 tagcataaac ggcgcgggaa gctggctggt agctaaccag gcgctgctgc taagttatgc
    12481 agtaaacatc gtggcggcac tcgcgatcat catcgttggt ttgattatcg cgcggatgat
    12541 ttccaacgcg gtgaatcgcc tgatgatctc ccgtaaaatc gatgccactg ttgctgattt
    12601 tctttctgca ttagtccgtt acggtattat cgcctttacg ctaatcgctg ccctgggacg
    12661 cgtgggtgta caaacagcgt cagtcattgc tgtactcggt gccgcaggct tagctgttgg
    12721 tctggctttg caggggtcac tttctaacct ggccgctggc gtgttacttg tcatgttccg
    12781 cccgttccgt gccggagaat atgttgacct cggcggcgta gccggtactg tgctgagtgt
    12841 gcagattttc tccaccacca tgcgtactgc agacggtaaa attatcgtta ttccgaacgg
    12901 taaaattatt gccggaaata ttattaactt ctcccgcgag ccagttcgcc gtaacgaatt
    12961 tattattggc gtggcgtatg attccgatat cgatcaggtt aagcagatcc tgaccaatat
    13021 tatccagtct gaagatcgca ttttgaaaga tcgcgaaatg actgtgcgcc tgaacgaact
    13081 tggtgcatcg tcgattaatt tcgtggtccg cgtctggagc aacagcggcg atctgcaaaa
    13141 cgtgtactgg gatgtgctgg agcgtattaa acgtgaattt gatgccgccg gtatcagctt
    13201 cccgtacccg caaatggatg tgaactttaa gcgggtgaaa gaagacaaag ctgcgtaatc
    13261 aacgctgagg ccagataata ctctctggcc tctctcttat tagttattct gattgccaat
    13321 taatattatc aatttccgct aataacaatc ccgcgatata gtctctgcat cagatactta
    13381 attcggaata tccaacgtgt tttcttatta ctttcaaggt cttgctcttg gggcagctat
    13441 gattctgccg ctcggcccac aaaatgcttt tgtgatgaat cagggcattc gtcgtcagta
    13501 ccacattatg attgccttac tttgcgctat cagcgatttg gtcctgattt gcgcggggat
    13561 ttttggtggt agcgcgttat tgatgcagtc gccgtggttg ctggcgctgg tcacctgggg
    13621 cggcgtagtc ttcttgctct ggtatggttt tggcgctttt aaaacagcga tgagtagtaa
    13681 tattgagtta gctagtgccg aagtcctgaa gcaaggcaga tggaaaatta tcgccaccat
    13741 gttggcagtg acctggctga atccgcatgt ttacctggat acttttgttg tactgggcag
    13801 ccttggcggg cagcttgacg tcgaaccaaa acgttggttt gcgctcggga caattagcgc
    13861 ctctttcctg tggttctttg gtctggctat tctcgcagcc tggctggcac cgcgcctgcg
    13921 cacggcaaaa tcacagcgca ttatcaatct ggtagtggga tgtgtcatgt ggtttatagc
    13981 cttgcagctg gcgagagacg gtattgctca tgcacaagcc ttgttcagtt aggcgctatc
    14041 tgatggaaaa ataaaacaga ggcgctaagc ttgcctccag aggtcctgaa ttttgggcaa
    14101 gtcaattaac acggagagac taacgtgaag ttcaaagtta tcgccctggc ggcattaatg
    14161 ggtattagcg ggatggcagc gcaggctaac gaattgccgg atggaccgca tattgtcacc
    14221 tccggtacgg caagtgtgga tgcggtgcca gacattgcca ctcttgcgat tgaagttaac
    14281 gtggccgcga aggatgccgc tacagccaag aaacaggcag atgagcgcgt cgcacaatac
    14341 atttccttcc ttgaactcaa tcagatcgcg aaaaaagata tcagctcagc gaatttacgc
    14401 acccagccag attatgacta tcaggatggt aaaagtatcc tgaaaggcta ccgagctgtg
    14461 agaacggtgg aagtcacgct ccgtcagtta gacaaactga attccttgct ggatggcgcg
    14521 ctgaaggcgg gtcttaacga aattcgttct gtgtcgctgg gcgtggcgca gccggatgcc
    14581 tataaagaca aagcgcgtaa ggcagcgatt gataacgcga ttcatcaggc gcaggaactg
    14641 gcgaacggct ttcatcgtaa actggggccg gtatatagcg tgcgctacca tgtttccaac
    14701 tatcagccca gcccaatggt gcggatgatg aaagccgatg ccgcgccggt gtctgcccag
    14761 gaaacttacg agcaggccgc tattcagttt gatgatcagg tcgatgtggt cttccagtta
    14821 gaacctgtgg atcaacaacc cgctaaaaca cctgcagcac aataatactt gtagcccgac
    14881 gtattcgtcg ggctaatgcc tttacccgat attgcctcct ttttctgtaa ttttctgtaa
    14941 aaccatgacg taatcaccta tctttgctcg cggtttcatc aaggataggc tgactgaaac
    15001 ggttaagcgg acaaggatgc ggggaggggg gatggacatt tttatttcga aaaagatgcg
    15061 caatttcatt ttattagcgc aaaccaataa tattgcccgg gcggcagaaa aaatccatat
    15121 gacggcttcg ccatttggta aaagcattgc cgcgctggaa gaacaaattg gctatacgct
    15181 atttacccgc aaagataata atatcagcct caataaagcc ggtcaggaac tgtatcaaaa
    15241 actgttcccg gtttatcaac gactttctgc gattgataat gaaatccata actcagggcg
    15301 tcgttcacga gaaattgtga ttggcataga taatacttat ccgacaatta ttttcgatca
    15361 gttgattagt ctcggagata agtatgaagg cgtgaccgcc cagccagttg agttcagcga
    15421 aaacggcgtt atcgataatc tttttgaccg tcagttagat tttattatct ccccacagca
    15481 tgtatctgca cgagttcagg aactggaaaa cctgactatc agtgaattac caccgctgcg
    15541 attgggcttt ctcgtttctc gccgttatga agagcgtcaa gagcaagagc tgcttcagga
    15601 gctgccctgg ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc
    15661 gaatatgcga ccttgtggga tcaatctcac cattatttat cgtccctaca gttttatggc
    15721 caaaatcagc gccgtggagc gcggacattt tctgacggtg atcccgcatt ttgcctggcg
    15781 actggtgaat ccggcaacgc tgaaatattt tgatgcgccg cacaggccga tgtatatgca
    15841 ggaatatctt tattcaatca gaaatcatcg ttataccgcc acgatgcttc agcatattgc
    15901 tgaagatcgt gacgggacaa accattaacc cagcatcgag ccggttgcaa ttaaattacg
    15961 gtgtaagtcg aagacgtggc taagatcgtg gtgaatatgt ccgccgggcg cattttccag
    16021 atagcgatgc agataatccc gatacatagg atgtgcacaa ttatcaatga tagtgcgggc
    16081 gcgttgaagc ggggaaagac cgcgcagatc ggcgatccct tgttcggtga tgatcacttt
    16141 gacgctgtgt tcgctgtgat caacatggct gcacattggc acgacggttg agatcttgcc
    16201 ttctttagca atcgacggag ccataaagat cgacagatac gcgttgcgtt caaaatcacc
    16261 gctgccgccg atgccgttca tcagatcgac cccggctacg tgcgttgaat ttgcatgccc
    16321 gtaaatatca aactccaggc cgacgttcag agcgatgacg cccagacgac ggatgatttc
    16381 cgggttattg gaaatctcct gcggacgcaa cacaatgcgg ctggcaaagt aatccatatt
    16441 gttgtaaatc ttgcgcaggg aatcggccga gattgtcagg ctggaggcgc tggccccgct
    16501 gattttgccg gtttccagta aatgcaccac cgattcctgt agcacttccg aatacatcat
    16561 gaacggagga atttccgggt tttcccccag acgcgccatt accgcattat tgatattgcc
    16621 cacgccactt tgcagcggca gaaattccgg cggaatacgc ccatgcgcca tttcctgcaa
    16681 taagaacgtg accacgttat cggcaatctg ctggcacatg ggattttgct tatccagcat
    16741 attaccggcg tcgggcaagt tggtttccac gacggcgaca atctttttcg gatcgatttg
    16801 cacatagcgg gtaccgacgc gatccattgc atggaagatc gacacgctat tgcgccgcgg
    16861 tggcgcgcca ggaatcacaa tatctgccag ttctgcaacg cgcggatcgt gatagtgatt
    16921 aagttcaatg atcactttct tcgctcgcag cagccaggtc ggcgcattac cgatcccgct
    16981 ggttaaccag actcgaccat ccggtgccag tgccgatgct tcaatgacgg caacatcaat
    17041 gtcgccgaag aaaccgtaat tgaccatttg cgccacttcg ctcaaatgca ggtcaacgaa
    17101 actcaccgcg ccctgattga tctttttacg taaactggac gatgtttgat atggcgcacg
    17161 ccaggaaaca gcatcggcgt cagaaagtac atcgtcagcg gcggcgctga ttgacgcccc
    17221 cgtcagcagg cgaatttgat acggcttttt ggcctcatgc tgttcgttag ctctgcgggc
    17281 aatcgcggcg ggtagggctt tcggcgaacc cgctggggta aagccgctaa atgccaccat
    17341 gtcgttatgc tggataattt ctgccgcttc atcggcggtc atccttgtcc actgagtttc
    17401 catttttact ccttcgttcg cagggattaa tgaccaacga aattaggttt acgtttttcg
    17461 aggaaagcgt tcatcccttc ctggtaatct tcgctgtcat aaaccgcgcg gcgcatcccc
    17521 tgaatacgtt caaattcatc ggagttcatg gtgtgtgctt cgcccagtac acgcagctct
    17581 tctttgataa cggcaatggc taacggcgct ttctcagaga tgtggtgcgc catttgtaag
    17641 gtgaaatctt ccagttcttc cacttccaca acatggttga ggatgccgac agccagcgcg
    17701 cgctgggcgg tgattggcga agcggtaaaa atcagctctt tgacaatgtg gaagcccgcg
    17761 tcgcgggtca ggttgtgaat gccgaccagg ttatacggga cgccgaggtt tacaggcgtc
    17821 attgagaagg ttgaggtact ggcggcgatg atcagatcgg aactcatgat catttcaaat
    17881 gcgccacccc aaacactacc ttccaccatc gaaatgatcg gtttcgggaa tttttggatc
    17941 atgcgggtga tttgacgcaa tggatcatca taggagagcg gatcgcgacc gccagacggc
    18001 agttcgtgaa tatcgtgacc tgcggagaag actttggagc cacttggcgc ccgcaaaatg
    18061 atacagcgaa tttccggccg gttgagatcg cttaacgcct gcataagatt atcaataaag
    18121 actttactta aggcattaag ttttcgtcca tagttaaact caatgaccgc cactttgttg
    18181 atagtggcaa cgttcacata ctgataagac ataaaaattc ctttaatcaa aatattgcgt
    18241 ctggataaat tcactgagct gccgcaggcc ggtgcgcggt gagagcgtat tgtttttgac
    18301 cgctaaaagc gtctggcggt aatagcgatc gaaatcttca ttcgcgaaca ggtgattcag
    18361 tacttcttct tcggtctgct tacgcagcca ttccaccgat tgttgttgcc gcacttgttg
    18421 taaacgacca ctggcagtta gcgcggtttt gaagtcgatg atggcgtgcc agatctcatc
    18481 gattccacgt ttttccagtg cgctacaagt cagaccccgt ggctgccatt cgtcgtattt
    18541 acgtcgcaga atatgcaggg cactctcgta catatgccgg gcaatggcga cattggtatg
    18601 gttatcgcta tcgtctttgt tgataacgag cagatcagcc acttccatca gccctttttt
    18661 aatgccctgc agatcatcgc cgccaccggc aatttgcaac gagataaaac agtccaccat
    18721 gcgggcgact tccgtttccg actgcgcgac gccaaccgtt tcgacaatca ctacgtcata
    18781 acccgccgct tcgcataaca gcattaattc ccgtgctcgc tgactggcac cgcccagatg
    18841 accggaggag ggtaccgggc gaataaacgc cgcctcggca cgcgccaggt cattcatgcg
    18901 ggttttatcc ccgagaatgc taccgccagt gaccgggctg ctgggatcga ccgcaataac
    18961 agcaaccttt aatccctcac gaatcaacaa catgccaaag gcttcaagaa aggtactttt
    19021 ccccgcgccg ggtgtgccgg taacgcccag tcgcagggcg ttaccacaga acggcataat
    19081 ggcgtcaagc agctgtgtac ttagcgcctg atgacgtggg tgacggcttt ccaccagcat
    19141 catggcttgg gcgagcgtgg cacgctcacc ctgacgtaag cggcgaatac tttctgccag
    19201 cgtggcttca ttaatcatga tgctggctta tcagattcag tacgtcgcgc acactgtcga
    19261 gcataggtgt acctggacca taaatcgccg ccacgccgcg ctcttgcagg aaggcgtaat
    19321 cctgcggcgg aatgacgcca cccgcgacca cgcagatatc ttcgcgtccc cattttttca
    19381 gcgcttcgac cagttccggg atcagcgttt tatgaccggc agccagtgag gatgcgccca
    19441 ctacgtgaac gtcgttttct acggccaggc gggcgatctc ttcaggtgta gagaacatcg
    19501 ggcttaaatc tacgtcgaaa ccgagatcgg aataggcgct ggcgatcact ttcgcgccgc
    19561 gatcgtgtcc atcctggccc atcttagcga tcagaatgcg cgggcgacga ccattgtcgg
    19621 caaggaactg ctccgtttgc gcaacaatgg catcgaactc ggaggccgat ttctcagact
    19681 gatgatagct ttgcgcaatc acgccggtaa cacactggca ccagataacg gtcgaaagcg
    19741 acttccagcg catcggaaat ttcacccagg gtggcgcgaa cgcgagcggc attaacagcg
    19801 gcagccagca ggttttcgtt atgctgtgcg gcgtgagtca gggcgttcaa cgcggcggtt
    19861 acggcggcat catcacgggt ggcgcgaatg cgttccagcg aagcaatttg ctcgttacgc
    19921 accatcacgt tgtcgatctc aagtacatcg gtttcgtctt cgtgatccag tttgtacttg
    19981 ttgacaccaa cgatgacacg cttgccctgg tcgatcagcg actgttcgcg cgctgaggcc
    20041 tcttcgatca ttcgttttgg cagacctgct tcgatcgctt tcgccatgcc accggcttcg
    20101 tcgatctgtt ggataatagc tctggcttgt ttgacgattt gatcggtcag cgactcaatg
    20161 taataggatc cggccagtgg atcgacggtg tggcagagtt ctgattcttc ctggatgatg
    20221 atctgggtgt tgcgggcaat gcgtgctgag aaatcggtag gcaaaccaag cgcttcgtca
    20281 aaggcgttgg tatgcagtga ctgagtaccg cccagcgtcg cagccagtgc ttcaatggtg
    20341 gtgcggataa cgttgttata cggatcctgt tcagtcaggc tccagcctga ggtctggcag
    20401 tgggtacgca gcgccagtga tttcggatcc tgtgcgccaa atccactgac cgcttcgctc
    20461 cataaataac gtgccgcacg caacatggcg acgttcataa acagatccat gccgataccg
    20521 aagaataacg acaggcgagg agcgaagtca tcaattttca gtccggcaga gattgctgct
    20581 ttgatgtact caatcccatc ggcgagcgta aatgctacct gctgcacgca gttggcaccc
    20641 gcttccccca tgtggtaacc gctgatactg atggtattaa atcgcggcat gttgccggaa
    20701 caccaggcga tgatgtcggc gataatgcgc attgacggtt ttggtgggta aatataggtg
    20761 ttgcggcaga ggtactcttt gagaatatcg ttttgaatgg tgccggtcag tttatcaggt
    20821 gtaacacctt gctcttctgc ggcgacgata taaaacgcca gtactggtag cactgcgcca
    20881 ttcatggtca tcgaaaccga cattttatcc agcgggatct ggtcgaacag gactttcata
    20941 tcttccacgg tgtcgatagc gacgcccgct ttgccgacgt cacccgccac gcgtgggtta
    21001 tcggagtcgt agccacggtg ggtggcaagg tcaaacgcaa cggaaagacc tttttgcccg
    21061 gcggccaggt tacggcgata aaaagcgttg gactcttttg ctgttgaaaa accagcatac
    21121 tgacggatgg tccacggttg ggcggtatac atagtggcac gcgggccacg aacgtagggc
    21181 ggcaaaccag gaagggtacc tgtcacctcc agattatcga gatcggcttc ggtatacagc
    21241 ggcttgatgg cgatcccttc cgcggtttgc tgaaccagcg agtcgacagt tttctcccga
    21301 cggctcaatt ccttgttggc aagctgttgc cactcctgct cgttagacat tatttgctcc
    21361 gtaaaacgca atcaaaatga gggtatttgc gcttacggtg agctttttac gtgggtttgt
    21421 cgccagcaaa aaaaatgcat tcagacgtcg ttttttgccg cctgagattt gtctattttg
    21481 tgatgtgatt caaggaatac aaaaagaagt gaggacaacg cctgatatgt gctatcaggc
    21541 attatttgat ggattaatcc tgacgaagga ctttgtgacc ataatcgagt aacgcatcag
    21601 tgactttacg catcatgcgg ctttcaggag caaagcggtg ccagtagagc atccgtcgtt
    21661 gaaatagccc aggcgttaag tcaatcagtt caccgctggc cagctctttc tcgatttgca
    21721 ggtgcgggat catacagcag gtggtgccct ggcgagcaag ttgtacgaac gcttctgaag
    21781 aattaacgat atggcagggt acgctgccag gaggaagatc gaagttttgc tgcaaaaagg
    21841 cctggtgcat atcgtcaaga tggtcaaacg cgaccactgg cgctttcaat aatgccgaac
    21901 gcgttacgcc gttaggaaaa tatttttcgg caaacggttt tgagctgacg aacagatagt
    21961 cgagcgcacc gagtttatcg acaagacaac tcggcagcgc ctgatgttga atactcaccg
    22021 cgccgaccac ttcgccgcgg cgcagacgtt cctgagtacg ggtttcatct tctacctgca
    22081 agttgaggcg gataggcgaa tcagccaaca caggagccag tgccggaagc aaccacgtcg
    22141 ccagactgtc ggcgttgacc gccagtgaaa gcagcagcgg agtcgaaccg gtttgttcat
    22201 cgcccagcca ctcttcttcc agcaactcca cttggcgcag cagtgccagc agtttttgcc
    22261 cttgttccgt cgggcgcggc ggtacggtac gcaccaacag cggctgcccg aacatatttt
    22321 ccagttgctt aatgcgctgt gagacggctg attgtgtaat gcacagcttt tgtgccgcgc
    22381 gctcaaatcc tcgttcacgt atcaccgcat ccagtgcctg taatgttctg tagtccgggc
    22441 gtttcattgc tgttgtctgc tcctggtaaa tgctttttcc tgcactatga cacaattttg
    22501 tgtcaggttg catataccga agccgtcggc gggtaacatt tgtatcaatg gttaaacgcg
    22561 cagcttaaaa gatcagggat ttgcgctatt gcatttcgct tagcgacttc gcgccctggt
    22621 tatgttgaat ggcgtggcgt tattgcctct gttcgcaccg tggagcggca tacccagacc
    22681 gttgtatgct ttatttgcgg ggcgaaaggg gatgcccgcc attgcgcgat attttatgga
    22741 tgagttaacc acccggcttg ccaacggggt ctgaatcgct ttttttgtat ataatgcgtg
    22801 tgaaatttca taccacaggc gaaacgatca tgacgcagga tgaattgaaa aaagcagtag
    22861 gatgggcggc acttcagtat gttcagcccg gcaccattgt tggtgtaggt acaggttcca
    22921 ccgccgcaca ctttattgac gcgctcggca cgatgaaagg ccagattgaa ggggccgttt
    22981 ccagttcaga tgcttccact gaaaaactga aaagcctcgg cattcacgtt tttgatctca
    23041 acgaagtcga cagccttggc atctacgttg atggcgcaga tgaaatcaac ggccacatgc
    23101 aaatgatcaa aggcggcggc gcggcgctga cccgtgaaaa aatcattgct tcggttgcgg
    23161 aaaaatttat ctgtattgcc gacgcctcca agcaggttga tattctgggt aaattcccgc
    23221 tgccagtaga agttatcccg atggcacgta gtgcagtggc gcgccagctg gtgaaactgg
    23281 gcggtcgtcc ggaataccgt cagggcgtgg tgaccgataa tggcaacgtg atcctcgacg
    23341 tccacggcat ggaaatcctt gacccgatag cgatggaaaa cgccataaat gcgattcctg
    23401 gcgtggtgac tgttggcttg tttgctaacc gtggcgcgga cgttgcgctg attggcacac
    23461 ctgacggtgt caaaaccatt gtgaaatgat ctgacggggg aacctccccc gttaaaaaaa
    23521 ttctcttcat taaatttggt gacatgtgtc acgcttttag caggcaattg tcgattgctc
    23581 taaataaatc ctctaaacca gcatattcat ccaagaatta cctttgcgtg atatttcctc
    23641 aacatcgcga cgcaaacgtt catattgccg caatattatt ttttgatatg ttgaaaggcg
    23701 gatgcaaatc cgcacacaac atttcaaaag acaggattgg gtaaatggca aaggtatcgc
    23761 tggagaaaga caagattaag tttctgctgg tagaaggcgt gcaccaaaag gcgctggaaa
    23821 gccttcgtgc agctggttac accaacatcg aatttcacaa aggcgcgctg gatgatgaac
    23881 aattaaaaga atccatccgc gatgcccact tcatcggcct gcgatcccgt acccatctga
    23941 ctgaagacgt gatcaacgcc gcagaaaaac tggtcgctat tggctgtttc tgtatcggaa
    24001 caaaccaggt tgatctggat gcggcggcaa agcgcgggat cccggtattt aacgcaccgt
    24061 tctcaaatac gcgctctgtt gcggagctgg tgattggcga actgctgctg ctattgcgcg
    24121 gcgtgccgga agccaatgct aaagcgcacc gtggcgtgtg gaacaaactg gcggcgggtt
    24181 cttttgaagc gcgcggcaaa aagctgggta tcatcggcta cggtcatatt ggtacgcaat
    24241 tgggcattct ggctgaatcg ctgggaatgt atgtttactt ttatgatatt gaaaacaaac
    24301 tgccgctggg caacgccact caggtacagc atctttctga cctgctgaat atgagcgatg
    24361 tggtgagtct gcatgtacca gagaatccgt ccaccaaaaa tatgatgggc gcgaaagaga
    24421 tttcgctaat gaagcccggc tcgctgctga ttaatgcctc gcgcggtact gtggtggata
    24481 ttccggcgct gtgtgatgcg ctggcgagca aacatctggc gggggcggca atcgacgtat
    24541 tcccgacgga accggcgacc aatagcgatc catttacctc tccactgtgt gaattcgaca
    24601 acgtccttct gacgccacac attggcggtt cgactcagga agcgcaggag aatatcggcc
    24661 tggaagttgc gggtaaattg atcaagtatt ctgacaatgg ctcaacgctc tctgcggtga
    24721 acttcccgga agtctcgctg ccactgcatg gtggtcgtcg tctgatgcac atccacgaaa
    24781 accgtccggg cgtgctaact gcgctgaaca aaatcttcgc cgagcagggc gtcaacatcg
    24841 ccgcgcaata tctacaaact tccgcccaga tgggttatgt agttattgat attgaagctg
    24901 acgaagacgt tgccgaaaaa gcgctgcagg caatgaaagc tattccgggt accattcgcg
    24961 cccgtctgct gtactaattc cccttctctg aaaattaacg ggcaggtcac tgacttgccc
    25021 gtttttttat cccttctcca caccgttgcc gcgttgcgca ttctactttg cgagtctgct
    25081 ccagaaaata aatcaaagtc agatcaatgc gttgtgttta tttaatgggg atttacgatg
    25141 gcgggacagc atggggctgt aacggtaaag ccctcaccga agcgagggct taaaggagaa
    25201 gggttatgat gcgacttgtc atcatactga ttgtactgtt actgataagt ttcggagctt
    25261 attaacagtc agtctcaggg gaggagtcat cctcccttac ccttactcac taaattaggt
    25321 caaagaatca acgatgtcaa tcagggcgat gcggggtgca tcgccctgaa ccgaactttt
    25381 tgatagccac ttcatcgccc acgtttactg ccgtaataga tcctttttcc caaaaattat
    25441 ctctgttttg catggggttt ttcgagcacg ctcgaagaaa agatttaaag gtagatcaat
    25501 gcgttgtggt tatttaaaga gagcttacga tagctgggca gcatggggct gtaacggtaa
    25561 agccctcacc gaagcgaggg cttgaaggag aagggttatg atgcgacttg ccatcatact
    25621 gattgtactg ttactcataa gtttcccagc ttattaacag tcagtcacag gggaggagca
    25681 atcctccctt acccttactc actaaattag gttaaagaat caacgatgtc aatcagggcg
    25741 atgcaggtgt atcgccctta ccactcccag actttcgacg gtgtaaccac cgcaggaaga
    25801 gggatatccc actcttcaac ggggagtttt tccaccaact gacaatcatg cgcataaccc
    25861 accggttgcg ttttatagtg ctgccagttt tgtaaggtcc gatcataaaa accaccgccc
    25921 attcccaggc gctgaccgta ctcatcaaag gcgaccagcg gtgtgatcag cacgtctaat
    25981 cgggaaaggg gtagcacgtc acgcacatcc agttttggct catggatctt caacctgttc
    26041 atcaccagtt cgctttgcgg atggtaattc aggaacagca aattaccggc actaaaggga
    26101 tgcaaaactg gaagatatac gcgcttaccg gcgcgccaga gttgttctat tagcggctgg
    26161 gtgtcgagtt caccatcaaa agagaggaat acagcgaccg tatgcgccat caccaccggg
    26221 ggataagtca tcatccgggt agcggcttgt tgacccattt ccagctgttg ttccggcgtt
    26281 aacgcacgac gacgttgccg aatcattttg cggatttctt gtcgggataa tgtcagtggg
    26341 agctctggta gttgcgtcat ggctggtgcc agataagaag ggaatctccg agatgccgcc
    26401 gcaggctgta acccttgaac ccttggttca aggtgaatgt gtcgtcgcag ttttaaggct
    26461 tctcggacga accgagcatg ctcaccaacc gcggagcgcc acattcttgt ggtatgaaat
    26521 atcggctcag gggactggcc cgcttgcgaa catctcagag aaattttgtc ttcacggtta
    26581 ctctaccaca gtaaaccgaa aagtgtcatt caaagttttg gttagttttt tcggtgatgc
    26641 gaccttgttc aagtaacgct tgttctatgg tctgctgcag catccgaata cgctgttcca
    26701 tacttgccgc gtagtcacga gtctttgctt tttcttgcgc taactcatag ctgatattca
    26761 atgcggcaat gaagaccaac tgttcagtat ttgtgactct agtgcgttct ttcagatctt
    26821 gcaaccgttg gttcagatcg tccgctgcct gattcaacgc atccctttgg tcaggcgggc
    26881 agttcacacg cagtgaacgg ccaaaaattt ggatatcgac gggttgtgca gacatgccac
    26941 cttcctgctg attgactgcg ctgccttcgt ctttagaccc atggtctgcg aaggggcgac
    27001 actatagcta ccctgatgag aagagacaag cccttttctg gtccaccagg gcccaaagtg
    27061 gtagcatatc atgaatattc ctccctttga cgacgaatgc ttatgtctat acagaacgaa
    27121 atgcctggtt acaacgaaat gaaccagtat ctgaaccaac aagggacggg tctgacccct
    27181 gccgagatgc atggtttaat cagtgggatg atatgtggcg gtaacgatga cagctcatgg
    27241 ctgccgctac ttcacgacct gacgaacgaa ggcatggctt tcggtcatga gctggcacag
    27301 gcactgcgta aaatgcactc tgccaccagc gatgccctgc aggatgacgg cttccttttt
    27361 cagctttatc tgcctgatgg cgatgatgtc agcgttttcg atcgggctga tgcactggca
    27421 ggttgggtca atcacttcct gcttggtctt ggcgttacgc aaccgaagct ggacaaagtg
    27481 accggcgaaa ccggtgaagc catcgacgat ctgcgtaaca tcgcgcagct gggctacgac
    27541 gaagacgaag atcaggaaga gcttgaaatg tcgcttgaag agatcatcga atacgttcgt
    27601 gttgccgcgc tgttatgcca cgacaccttt actcatccgc aaccgaccgc gccagaagta
    27661 caaaaaccga ctctacacta aaaacaaaaa acgtaaggag agtgttatga gtgagatatc
    27721 ccggcaagag tttcagcgtc gccgtcaggc cctggtggag caaatgcaac ccggcagcgc
    27781 cgcgctgatt tttgctgcac cagaagtaac acgtagcgcc gacagcgaat acccctatcg
    27841 tcagaacagt gacttctggt acttcaccgg ctttaacgaa ccggaagcgg tgctggtgct
    27901 gattaaaagc gatgacactc ataaccacag cgttctgttt aaccgcgttc gcgacctgac
    27961 ggcggagatc tggtttggcc gtcgcttagg ccaggatgcc gcgccagaga aactgggcgt
    28021 tgaccgcgca ctggcattca gcgaaatcaa tcagcaactt tatcaactac ttaacggcct
    28081 ggatgtggtt taccatgccc aggacgaata tgcatatgct gatgaaatcg tgaacagtgc
    28141 gctggaaaaa ctgcgtaaag gttcgcggca aaatctcacc gcaccggcaa cgatgatcga
    28201 ctggcgtcct gttgttcatg aaatgcgcct gttcaaatcg ccagaagaga ttgccgtact
    28261 ccgccgcgcg ggagaaatca ccgccatggc acatacacgg gcgatggaaa aatgccgtcc
    28321 gggaatgttc gagtaccatc tggaaggcga aattcaccac gaatttaacc gccacggtgc
    28381 gcgctatcct tcctataaca ccattgtagg cagcggtgaa aacggctgca ttctgcacta
    28441 caccgaaaac gagagtgaac tgcgcgacgg tgacctggtg ttgattgacg cgggttgtga
    28501 atacaaaggt tacgcgggcg atattacccg caccttcccg gtcaacggca aattcaccca
    28561 ggcccagcgt gaaatctacg acattgtgct ggagtctctc gaaaccagcc tgcgcctgta
    28621 tcgtccggga acttccattc tggaagtcac tggtgaagtg gtgcgcatca tggttagcgg
    28681 cctggtaaaa ctcggcatcc tgaaaggtaa tgttgatgaa ctgatcgctc agaacgccca
    28741 tcgtcctttc tttatgcatg gccttagcca ctggttagga ctggatgtcc atgacgttgg
    28801 cgtttatggt caggatcgct cgcgcattct ggaaccgggg atggtactga ccgtagagcc
    28861 tggtctgtat atcgcgccgg atgcggatgt gccagaacaa tatcgcggta tcggcattcg
    28921 tattgaagac gacattgtga ttaccgaaac cggcaacgaa aacctcaccg ccagcgtggt
    28981 gaaaaagccg gaagaaatcg aagcgttgat ggctgctgcg agaaagcaat gagcgtaatc
    29041 atcgtcggtg gcggcatggc gggcgcgacg ctggcgctgg ctatttcccg gttaagtcac
    29101 ggggcgctgc cggtacattt gattgaagcg actgcgccag agtcacatgc tcatccgggc
    29161 tttgatggac gagcgatagc gctggcggcg ggtacctgtc agcaactggc gcgcatcggc
    29221 gtctggcaat ctctggctga ttgcgcaacc gccatcacta ccgtgcatgt cagcgatcgt
    29281 ggtcatgctg gattcgtcac cctcgtcgca gaagattacc aactggcggc gctggggcag
    29341 gttgtcgaat tgcataatgt cgggcaacgg ctgtttgcat tgctgcgaaa agcacctggc
    29401 gtaatgctgc attgccccga tcgcgtagct aacgttgccc gtactcagag tcacgttgaa
    29461 gtgacgctgg agagcggcga gatgctgacg ggccgcgtgc tgatagccgc tgatggcacc
    29521 cattcagcgt tagccaccgc gtgcggcgtt gattggcagc aggagcctta cgaacaactg
    29581 gcagtgattg ccaacgttgc tacttctgtt gcgcacgaag gccgcgcttt tgaacgcttc
    29641 acgcaacatg gcccgctggc gatgttgccg atgtctgacg gacgctgttc gctggtctgg
    29701 tgtcatccac tggaacggcg cgaagaggtg ctgtcgtgga gcgacgagaa gttttgccgt
    29761 gaactccagt ctgcctttgg ctggcgactg gggcaaatta cccacgccgg taaacgcagt
    29821 gcttatccgc tggcattaac ccgcgccgcc agaccgatta cccatcgcac tgtgctggtg
    29881 ggcaatgcgg cgcaaactct gcacccgatt gccgggcaag ggtttaacct cggtatgcga
    29941 gatgtcatga gccttgcgga aaccctgact caggcgcatg agcgcggaga agacatcggt
    30001 gattacggca tattgtgccg ctatcagcag cgtcgacaga gcgatcgcga agcaaccatc
    30061 ggcgtcacgg acagcctggt acatcttttt gccaaccgtt gggcaccgct ggttgtcggg
    30121 cgcaacatcg gactgatgac gatggaatta ttcaccccgg cacgcgatgt gctggcgcag
    30181 cgcaccctcg gttgggtggc gcgttgaagg cggttttaag gaataaacaa tgcaaagtgt
    30241 tgatgtagcc attgttggcg gcggcatggt ggggctggcg gttgcctgcg gcatacaggg
    30301 gagcggctta cgcgttgccg tactggagca gcgcgtaccg gaacctctgg cggtagatgc
    30361 actaccacaa ctgcgcgttt cggctatcaa tgccgccagc gaaaaattac tcacccgtct
    30421 tggcgtctgg caggacattc tcgctcgtag ggctagttgc tatcacggca tggaagtgtg
    30481 ggacaaagac agttttggtc acatctcgtt tgacgatcaa agcatgggct atagccatct
    30541 tgggcacatc gttgaaaact cagtgattca ctacgcgctg tggaacaaag cgcagcagtc
    30601 gtcagatatt actctgttgg cccccgcaga attacagcag gtcgcctggg gagaaaatga
    30661 aaccttcctg acgctgaaag atggcagcat gttaacggcg cgtctggtga ttggcgcgga
    30721 cggtgctaat tcctggttgc gcaacaaagc agatattccg ctgactttct gggattatca
    30781 gcatcacgcg ctggtagcga ccattcgcac ggaagaaccg catgatgcgg tggcgcggca
    30841 ggttttccat ggcgaaggca ttctggcctt tttaccgctt agcgatccgc atctttgctc
    30901 gattgtctgg tcactgtcgc cagaggaagc gcagcggatg cagcaggcaa gtgaagacga
    30961 atttaatcgc gcgttaaata tcgcttttga taatcgcctt ggcttatgca aggttgagag
    31021 cgcgcgtcag gtgttcccgc tgacggggcg ttatgcgcgc cagtttgccg cacaccgtct
    31081 ggcgctggtg ggcgacgccg cgcataccat tcacccactg gcggggcagg gcgttaatct
    31141 cggatttatg gacgccgcag agctgattgc tgaactgaaa cggttgcatc gtcagggtaa
    31201 agacatcggg cagtacattt acctgcgccg ctatgaacgt agccgcaagc acagcgcggc
    31261 gctgatgctg gctggtatgc agggttttcg cgatctcttt tccggcgcta atccggcgaa
    31321 aaaactgctg cgtgatattg gtttgaaact ggccgacacg cttcctggcg ttaagccgca
    31381 acttatccgc caggcaatgg gattaaacga tttgcctgaa tggctgcgtt aaaaatttct
    31441 cctctgttgt ttatttgata cccatcacac tttcatctcc cggttttttc gccgggagat
    31501 tttcctcatt tgaaataaac taatttcacc tccgttttcg cattatattt tctaatgcca
    31561 tgattttttg atttagtgtt ttttgacatt tttttagctc ttaatattgt cttatccaaa
    31621 ttgactttct catcacatca tctttgtata gaaattggtg tattttttgg ttttttattc
    31681 tgtcgcgatt tttgcatttt ttaaccataa gctaatgtga tgatcaattt taccttatgg
    31741 ttaacagtct gtttcggtgg taagttcagg caaaagagaa cgattgcgtt ggggaccggg
    31801 agtggctccg atgctgggtt ttgtggtgat aatttcacca tgaaaaagtt gtcagccccg
    31861 cttattcaat gaggacaaga tggcacaaca gactcctttg tacgaacaac acacgctttg
    31921 cggcgctcgc atggtggatt tccacggctg gatgatgccg ctgcattacg gttcgcaaat
    31981 cgacgaacat catgcggtac gtaccgatgc cggaatgttt gatgtgtcgc atatgaccat
    32041 cgtcgatctc cgcggcagcc gcacccggga gtttctgcgt tatctgctgg cgaacgatgt
    32101 ggcgaagctc accaaaagcg gcaaagccct ttactcgggg atgttgaatg cctctggcgg
    32161 tgtgatagat gatctcatcg tctactactt tactgaagat ttctttcgcc tcgttgttaa
    32221 ctccgccacc cgcgaaaaag acctctcctg gattacccaa cacgctgaac ctttcggcat
    32281 cgaaattacc gttcgtgatg acctttccat gattgccgtg caagggccga atgctcaggc
    32341 aaaagctgcc acactgttta atgatgctca gcgtcaggcg gtggaaggga tgaaaccgtt
    32401 ctttggcgtg caggtgggcg atctgtttat tgccaccact ggttataccg gtgaagcggg
    32461 ctatgaaatt gcgctgccca atgaaaaagc ggccgatttc tggcgtgcgc tggtggaagc
    32521 gggcgttaag ccctgcggtc tgggggcgcg tgacacgctg cgtctggaag cgggcatgaa
    32581 tctttatggt caggagatgg acgaaactat ttctccttta gccgccaaca tgggctggac
    32641 tatcgcctgg gaaccgacag atcgtgactt tatcggtcgt gaagccctgg aagtgcagcg
    32701 tgagcatggt acagaaaaac tggttggtct ggtgatgacc gaaaaaggcg tgctgcgtaa
    32761 tgaactgccg gtacgcttta ccgatgcgca gggcaaccag catgaaggca ttatcaccag
    32821 cggtactttc tccccgacgc tgggttacag cattgcgctg gcgcgcgtgc cggaaggtat
    32881 tggcgaaacg gcgattgtgc aaattcgcaa ccgtgaaatg ccggttaaag tgacaaaacc
    32941 tgtttttgtg cgtaacggca aagccgtcgc gtgatttact tttttggaga ttgattgatg
    33001 agcaacgtac cagcagaact gaaatacagc aaagaacacg aatggctgcg taaagaagcc
    33061 gacggcactt acaccgttgg tattaccgaa catgctcagg agctgttagg cgatatggtg
    33121 tttgttgacc tgccggaagt gggcgcaacg gttagcgcgg gcgatgactg cgcggttgcc
    33181 gaatcggtaa aagcggcgtc agacatttat gcgccagtaa gcggtgaaat cgtggcggta
    33241 aacgacgcac tgagcgattc cccggaactg gtgaacagcg aaccgtatgc aggcggctgg
    33301 atctttaaaa tcaaagccag cgatgaaagc gaactggaat cactgctgga tgcgaccgca
    33361 tacgaagcat tgttagaaga tgagtaacgg ctttattcct cttctgcggg agaggatcag
    33421 ggtgaggaaa atttatgcct caccctcact ctcttcgtaa ggagagaggt tcacaattca
    33481 ctgcacgttt caggaaccat cgctcatgac acagacgtta agccagcttg aaaacagcgg
    33541 cgcttttatt gaacgccata tcggaccgga cgccgcgcaa cagcaagaaa tgctgaatgc
    33601 cgttggtgca caatcgttaa acgcgctgac cggccagatt gtgccgaaag atattcagct
    33661 tgcgacacca ccgcaggttg gcgcaccggc gaccgaatac gccgcactgg cagaactcaa
    33721 ggctattgcc agtcgcaata aacgcttcac gtcttacatc ggcatgggtt acaccgccgt
    33781 gcagctaccg ccggttatcc tgcgtaacat gctggaaaat ccgggctggt ataccgcgta
    33841 cactccgtat caacctgaag tctcccaggg ccgccttgaa gcactgctca acttccagca
    33901 ggtaacgctg gatttgactg gactggatat ggcctctgct tctcttctgg acgaggccac
    33961 cgctgccgcc gaagcaatgg cgatggcgaa acgcgtcagc aaactgaaaa atgccaaccg
    34021 cttcttcgtg gcttccaatg tgcatccgca aacgctggat gtggtccgta ctcgtgccga
    34081 aacctttggt tttgaagtga ttgtcgatga cgcgcaaaaa gtgctcgacc atcaggacgt
    34141 cttcggcgtg ctgttacagc aggtaggcac taccggtgaa attcacgact acactgcgct
    34201 tattagcgaa ctgaaatcac gcaaaattgt ggtcagcgtt gccgccgata ttatggcgct
    34261 ggtgctgtta actgcgccgg gtaaacaggg cgcggatatt gtttttggtt cggcgcaacg
    34321 cttcggcgtg ccgatgggct acggtggccc acacgcggca ttctttgcgg cgaaagatga
    34381 atacaaacgc tcaatgccgg gccgtattat cggtgtatcg aaagatgcag ctggcaatac
    34441 cgcgctgcgc atggcgatgc agactcgcga gcaacatatt cgtcgtgaga aagcgaactc
    34501 caacatttgt acttcccagg tactgctggc aaacatcgcc agcctgtatg ccgtttatca
    34561 cggcccggtt ggcctgaaac gtatcgctaa ccgcattcac cgtctgaccg atattctggc
    34621 ggcgggcctg caacaaaaag gtctgaaact gcgccatgcg cactatttcg acaccttgtg
    34681 tgtggaagtg gccgacaaag cgggcgtact gacgcgtgcc gaagcggctg aaatcaacct
    34741 gcgtagcgat atcctgaacg ccgtggggat cacccttgat gaaaccacca cgcgtgaaaa
    34801 cgtaatgcag cttttcagtg tgctgctggg cgatagccac gggctggaga tcgacacgct
    34861 ggacaaagac gtggctcacg acagccgctc tatccagcct gcgatgctgc gcgacgacga
    34921 aatcctcacc catccggtgt ttaatcgcta tcacagcgaa accgaaatga tgcgctatat
    34981 gcactcgctg gagcgtaaag atctggcgct gaatcaggcg atgatcccgc tgggttcctg
    35041 caccatgaaa ctgaacgccg ccgccgagat gatcccaatc acctggccgg aatttgccga
    35101 actgcacccg ttttgcccgc cggaacaggc tgaaggttat cagcagatga ttgcgcagct
    35161 ggctgactgg ctggtgaaac tgaccggtta cgacgccgtt tgtatgcagc cgaactccgg
    35221 cgcacagggc gaatacgcgg gcctgctggc gattcgtcat tatcatgaaa gccgcaacga
    35281 agggcatcgc gatatctgcc tgatcccggc ttctgcgcac ggaactaacc ccgcttctgc
    35341 acatatggca ggaatgcagg tggtggttgt ggcgtgtgat aaaaacggca acatcgatct
    35401 gactgatctg cgcgcgaaag cggaacaggc gggcgataac ctctcctgta tcatggtgac
    35461 ttatccttct acccacggcg tgtatgaaga aacgatccgt gaagtgtgtg aagtcgtgca
    35521 tcagttcggc ggtcaggttt accttgatgg cgcgaacatg aacgcccagg ttggcatcac
    35581 ctcgccgggc tttattggcg cggacgtttc gcacctcaac ctgcataaaa ctttctgcat
    35641 tccgcacggc ggtggtggtc cgggtatggg accgatcggt gtgaaagcgc atctggcacc
    35701 gtttgtaccg ggtcatagcg tggtgcaaat cgaaggcatg ttaacccgtc agggcgcagt
    35761 ttctgcggca ccgttcggta gcgcctctat cctgccaatc agctggatgt acatccgcat
    35821 gatgggcgca gaagggctga aaaaagcaag ccaggtggca atcctcaacg ccaactatat
    35881 tgccagccgc ctgcaggatg ccttcccggt gctgtatacc ggtcgcgacg gtcgcgtggc
    35941 gcacgaatgt attctcgata ttcgcccgct gaaagaagaa accggcatca gcgagctgga
    36001 tattgccaag cgcctgatcg actacggttt ccacgcgccg acgatgtcgt tcccggtggc
    36061 gggtacgctg atggttgaac cgactgaatc tgaaagcaaa gtggaactgg atcgctttat
    36121 cgacgcgatg ctggctatcc gcgcagaaat tgaccaggtg aaagccggtg tctggccgct
    36181 ggaagataac ccgctggtga acgcgccgca cattcagagc gaactggtcg ccgagtgggc
    36241 gcatccgtac agccgtgaag ttgcggtatt cccggcaggc gtggcagaca aatactggcc
    36301 gacggtgaaa cgtctggatg atgtttacgg cgaccgtaac ctgttctgct cctgcgtacc
    36361 gattagcgaa taccagtaat tcactgattc gagtattttc taaaggcgct tcggcgcctt
    36421 tttagtcaga tgacaaagta caaaagtgct cagacagtcc cctcgcccct ttggggagag
    36481 ggttagggtg aggggaacag gccggcactg gcgcgaatat ttaccctcac cccgaccctc
    36541 tccctgaaag ggcgaggggg aaaagcgtgc caacattgaa gattgagcca gtttgttagc
    36601 aatctcaaag atacgtcaac gaattaattt ttctcggaaa aacaaatggc tatagcactt
    36661 gtgaccggtg gcagttgcgg catcgggcgg gcaactgcat tactgttggc gcaagaaggg
    36721 tatacggtgg cggttaatta tcagcaaaac ctccacgcgg cgcaggaagt gatgaactta
    36781 ataatgcaag ccggtggcaa agcattcgtg ctccaggcgg atatcagcga cgaaaaccag
    36841 gtcgttgcga tgtttacagc aatcgatcag cacgatgaac cgctagcagc gctggtcaat
    36901 aacgccggga tcttgtttac ccagtgcacc gttgaaaacc ttaccgcaga gcgaatcaac
    36961 cgagtacttt ccaccaacgt gacgggatat tttctctgct gccgcgaggc ggtaaaacgc
    37021 atggcgctta aaaatggtgg cagtggcggc gctatcgtca atgtctcttc ggtggcctca
    37081 cgattgggtt cgccagggga atatgttgat tacgcggcat cgaaaggggc gattgatacg
    37141 ttaaccaccg gactatcgct ggaagtcgcc gcgcagggga tccgcgttaa ctgcgtgcgg
    37201 ccagggttta tttataccga aatgcacgcc agcggcggcg agcctggacg cgtcgatcgc
    37261 gttaagtcga acatccccat gcagcgtggt ggacaggcag aagaggtcgc gcaggccatt
    37321 gtctggctac taagtgataa agcctcttac gtcacgggaa gttttatcga tttggcgggc
    37381 gggaaataaa atagggaagt tgtctgaccg gatgcaacaa gtattgcatc cggtacttca
    37441 tcgacttaaa gcttctcgcc gttgctggca atcacctctt tgtaccagtt aaagctcttc
    37501 ttacgtgaac gcgacatatc gccagtaccg tcgtcatgtt tattcacata gataaagccg
    37561 tagcgtttgc tgtactgccc ggtagtgaat gacacgcagt cgatgcagcc ccacggtgtg
    37621 tagcccatca gatccacacc atcgtaagtc actgcttttt tcatctcttc aatatgggcg
    37681 cgcaggtagt caatgcggta gtcgtcgttg atgctgccat cttcttccac tttgtcgtaa
    37741 gcgccaaaac cgttttcgac aataaacagc ggcctctgat aacgctcata cagttcgcaa
    37801 agggcatagc gcagacctac tggatcaatc tgccagcccc agtcagacgc tttaacatac
    37861 gggttcggta cgctgccttc aaaaccagag atcgcatcgc cggtgccgcc ttcggccttc
    37921 actgcgttgg tcatgtaata gctgaaacca agataatcgc aggtgccttc gcgcagtaca
    37981 tcgagatcgc cgtcttccat tttgatatta aatccgcgac gttcccactc gttcaataca
    38041 taggacgggt aatagccgca tagctgcaca tcggtaaaga cgtagcgttc gcgcatcgac
    38101 tcctgagcga acatcacatc gtccgggtta catgagtagg gatagagcgg caccatcgcc
    38161 agcatacagc cgactttcat ctccgggtta atgcgacgcg cggctttcac tgccagggcg
    38221 ctggcgacaa actggtgatg cagcacctgg tacatcgtct cttccgggtt gtcatgctcg
    38281 gtatacacca cgccggagca gcagtaacca aacagcggcg cacgccagtt acgctggttg
    38341 tttatttcgt tgaaggtcat ccagtatttg actttatgct tgtagcgttc gaataccact
    38401 tccgcgaaac ggacaaagaa atcaacgact ttacggttgg tccagctacc gtattgctgc
    38461 accagatgca gcggcatttc aaagtgggag agggttatca ccggttcgat gttgtatttc
    38521 agcagttcat cgaacagaga gtcgtaaaac ttcagccctt cttcgtttgg ctgagcttca
    38581 tcaccttttg gaaaaatacg cgtccaagcg atggaagtcc gaaaacattt gaagcccatt
    38641 tcggcaaata gcttgatgtc ttccttgtag tgaccataaa aatcaacggc ttcatggttt
    38701 ggatagtatt ttcctggcaa gacttctttg gtgatttcgc gcggcacgcc gtgtgcgcca
    38761 ccggtcagaa cgtcacaaat gctcggtcct ttgccgcctt tgttccagcc gccttcgacc
    38821 tgatgagcgg caacggcgcc accccataag aaatctttcg gtaaggtgag ttttttcact
    38881 atcataggct ccagatcagt caattcttac aggaaatagt accatgcagc caaacgacat
    38941 cacttttttt caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg
    39001 cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt cgtgtcggac gttttgaaga
    39061 tgacggttat ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacact
    39121 gacggaaaaa catgcagagc aggaaaatat gaccctgcct gaactgaaaa aggtcattgc
    39181 tgacatctat cccggtcaga cacaatttta tatgattgaa tttaaatgtc tttaaattga
    39241 actcacgcct gttagttgaa atttcgcatt taatttcatg atttttagga gattagtctc
    39301 aaatcagaat ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag
    39361 aggtatacat gtgcgtgtac gtttctctgg agtaagttat ggttcagaag cccctcatta
    39421 agcagggata ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggctgg
    39481 tgtttggtat cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg
    39541 ccacagcgat aaccagttac agcctctatg gcggcagtat gatcctgctg ttcctcgctt
    39601 cgacgctcta tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc
    39661 attgcgctat ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg
    39721 attctccgtt agcgcgcggg ttgatgattg ttatctggag cctggcattg ctggggattc
    39781 tgttcaaact gaccatcgcg caccgattca aaattttatc tctggtgact tatctggcga
    39841 tgggctggct gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg
    39901 ttaccttact ggcggtaggc ggcgtggttt attcgctcgg ggtgattttc tacgtctgca
    39961 aacgcattcc atacaaccat gccatctggc acggcttcgt gctcggcggt agtgtgtgcc
    40021 actttctggc gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg
    40081 ataagacgcg tcagcgtcgc atcaggcatc ggagcgcaac cgccggatgc ggcgtgaacg
    40141 ccttatccga tctacgggcc tcgtccctgt tgtgataaag gcatcaaatc ccagacatga
    40201 taagacgcgt cagcgtcgca tcaggcgata aatgcctgat gcgatacaga gatttactct
    40261 tcgagcgaat acggcagcgg ctcgatatgc agtgtattcg catcgtcgcg tacgcggaag
    40321 atgctgtccg gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc
    40381 agttttaccg cagccagcgc cgtaccggta cgacgccagt tctcgcccat tttcagctct
    40441 aagtcttcac cagcttccgg cagtcggctg gcactacctg ccagcaacca gagcgcgcgt
    40501 ttgttggcac cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc
    40561 ttaaagctga taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg
    40621 ttggcggcat caatcaccgg gaaaccagct tcaatgttta atgccagcca ctgttggcta
    40681 ttgttcagtt ccgcttcacc gcgcagttta tcggtcagca tattagcagt agcttcatcg
    40741 gttacgatca ggaaacgttc tgccgggtgt tcaaaccata gcaaagtggt cgcgccttct
    40801 ttgactacct gtttttcttt cgaaggcagt tcgctaaaga gatttgccag cgcggcgcgc
    40861 gcctgaaaac cggcaacacc aagcagcaca cgctcgtcgt ctggagcgat ggtcacttta
    40921 gagaataccg catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca
    40981 atccatgcaa agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttacctttg
    41041 gcgtcgcaat gggcggcgag caggtgctga tcttctgcca tctggctgac atctgctgtc
    41101 acctgaccct gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca
    41161 tcaagcgtca tcagcgtcag cggtaaacgg gcagaagccg taggctgacg tggaggaaaa
    41221 ggtgtaaaag ccataataga gtcctgagtt gcttaacggc gtattaatga ctaatggtaa
    41281 aagagctatg aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac
    41341 tgccaaaaaa atgaagccct tgaattgttg tgaattaatt tcgcgatcgc gatggtgttt
    41401 actgatattc ccgtaaaaga agtcgaaaaa cacgttacaa tagccgggta ctggattttc
    41461 gtgagacagg aagaagatgg acattaacaa caaagcccgc attcattggg catgccgccg
    41521 tggtatgcgc gaactcgata tttcaatcat gccgtttttc gaacatgagt acgacagctt
    41581 aagcgatgac gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cggacctgtt
    41641 taactggctg atgaatcacg gtaaaccggc cgatgcagaa ctggaaataa tggtccgact
    41701 catccagaca cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc
    41761 gcgcacagtg gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc
    41821 cctggccgct cagttacacc ccgttatgga tggtgttact ttcgctggtg gtatttgatt
    41881 gcgttcgcag ccagcggcgt attaatgctc gccaggggga aattcgcttg ttgatggacg
    41941 ggcgtttgcg ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga
    42001 gctgcatgat gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag
    42061 ccgacagcat ggacgaagcc gaatggcggg atttacggcg gattttgttg caacaagaga
    42121 cgcaaagata aagatgacgg caaagcatat gcgctaagcc gtcagtgaga tcaggcgtaa
    42181 tgctctgcca tttcgttgag gatttgctcg caccagctct gaatacgctc gtcgctaagg
    42241 tcatactggt tagtttcatc cagcgccaga cccacgaaca gttgcccgtc agcaatcacc
    42301 ggtttcgggc tggtaaattc atatccttcc gttggccagt agccgacgaa cttcacgcct
    42361 ttggtcgaga gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc
    42421 agttgatcgc caagcccata cagcgcaaca attttacctt caaggttcag gtcgtcgagc
    42481 tgatcccaga cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc
    42541 agaatgagca catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag
    42601 gtcaccagtt ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag
    42661 gtgctggaac cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc
    42721 ttttgcagct agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa
    42781 atgagtgacc ataaggggcg caagtgaaac aggatctggc acgcatcgag cagtttcttg
    42841 atgctctgtg gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc
    42901 tgtcaatgat ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg
    42961 acgatttgca ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag
    43021 cgcgtttgct gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg
    43081 aagacgatcc cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt
    43141 taagcgaagc gcaggtcgaa cgtttattac aggcgccatt aattgatctg ccactggagc
    43201 tacgcgataa agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg
    43261 tcgggctgac aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag
    43321 gcaacaaaga gcgcctggtg ccgttagggg aagaggcggt ttactggctg gaaacctatc
    43381 tggaacatgg gcgtccgtgg ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc
    43441 agcgtgcgca gcagatgacg cgacagacct tctggcaccg cattaaacat tatgccgtgc
    43501 tggcgggtat cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc
    43561 atttattaaa tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc
    43621 tttccaccac gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac
    43681 agcatcaccc gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta
    43741 tgttgtttac tttgttagcg gcgttttcag gctttgctca ggctgatgac gcggcaattc
    43801 aacaaacgtt agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg
    43861 gcatgaagac agttctgact aacagcggtg tgttgtacat caccgatgat ggtaaacata
    43921 tcattcaggg gccaatgtat gacgttagtg gcacggctcc ggtcaatgtc accaataaga
    43981 tgctgttaaa gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgccgcagg
    44041 aaaaacacgt catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg
    44101 agcaaatggc agactataac gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc
    44161 aggggctgga cagcgatgca gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa
    44221 acaaagcgtt tgatgatgtg atggcaggta aaagcgtcgc accagccagc tgcgacgtgg
    44281 atattgccga ccattacgca cttggcgtcc agcttggcgt tagcggtact ccggcagttg
    44341 tgctgagcaa tggcacactt gttccgggtt accagccgcc gaaagacatg aaagaattcc
    44401 tcgacgaaca ccaaaaaatg accagcggta aataattcgc gtgaaacaac agatacaact
    44461 tcgtcgccgt gaagtcgatg aaacggcaga cttgcccgct gaattgcctc ccttgctgcg
    44521 ccgtttatac gccagccggg gagtacgcag tgcgcaagaa ctggaacgca gtgttaaagg
    44581 tatgttgccc tggcagcaac taagcggtgt cgaaaaggcc gttgagatcc tttacaacgc
    44641 ttttcgcgaa ggaacgcgga ttattgtggt cggtgatttc gacgccgacg gcgcgaccag
    44701 cacggctcta agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt
    44761 accaaaccgt ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggcccatgc
    44821 ccgtggcgcg cagttaattg tcacggtgga taacggtatt tcctcccatg cgggagttga
    44881 gcacgctcgc tcgttgggca tcccggttat tgttaccgat caccatttgc cgggcgacac
    44941 attacccgca gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa
    45001 atcactggca ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca cctttttgcg
    45061 cgatcagggc tggtttgatg agcgcggcat cgcaattcct aacctggcag aactgctgga
    45121 tctggtagcg ctggggacag tagcggacgt cgtgccgctg gacgctaata atcgcattct
    45181 gacctggcag gggatgagtc gcatccgtgc cggaaagtgc cgtccaggga ttaaagcgct
    45241 gctggaagtg gcaaatcgcg atgcacaaaa actagccgcc agcgatttag gttttgcgct
    45301 gggaccgcgt ctcaatgctg ccgggcgact ggatgatatg tccgttggtg tggcgctgtt
    45361 gctgtgcgac aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca
    45421 gacgcgaaaa gagatcgaac aaggaatgca ggttgaagcc ctgaccctgt gcgagaaact
    45481 ggagcgcagc cgtgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca
    45541 gggcgttgtc ggtattctgg cttcgcgtat caaagagcgt tttcaccgtc cggttatcgc
    45601 ctttgcgcca gcaggtgacg gtacgctgaa aggttccggt cgctccattc aggggctgca
    45661 tatgcgtgat gcgctggagc gattagacac actctacccc ggcatgatgc tcaagtttgg
    45721 cggtcatgcg atggcggcgg gtttgtcgct ggaagaggat aaattcgaac tctttcaaca
    45781 acggtttggc gaactggtta ctgagtggct ggccccttcg ctattgcaag gcgaagtggt
    45841 gtcagacggt ccgttaagcc cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga
    45901 tgctggcccg tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccgtctgct
    45961 gcaacagcgg ctggtgggcg aacgtcattt gaaggtgatg gtcgaaccgg tcggcggcgg
    46021 tccactgctg gatggtattg cttttaatgt cgataccgct ctctggccgg ataacggcgt
    46081 gcgcgaagtg caactggctt ataagctcga tatcaacgag tttcgcggca accgcagcct
    46141 gcaaattatc atcgacaata tctggccaat ttagcgtcat cttctctata aaaaagagcg
    46201 tggattgggt acaatcccgc tcttatcacc gcattttgac tagctcaata aaagaaatca
    46261 gaccatgttt gaaattaatc cggtaaataa tcgcattcag gacctcacgg aacgctccga
    46321 cgttcttagg gggtatcttt gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg
    46381 ccgagctgga acagccggat gtctggaacg aacccgaacg cgcacaggcg ctgggtaaag
    46441 agcgttcctc cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag gggctggaag
    46501 atgtttctgg tctgctggaa ctggctgtag aagctgacga cgaagaaacc tttaacgaag
    46561 ccgttgctga actcgacgcc ctggaagaaa aactggcgca gcttgagttc cgccgtatgt
    46621 tctctggcga atatgacagc gccgactgtt acctcgatat tcaggcgggg tctggcggta
    46681 cggaagcaca ggactgggcg agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc
    46741 gtggtttcaa aactgaaatc atcgaagagt cggaaggtga agtggcgggt attaaatccg
    46801 tgacgatcaa aatctccggc gattacgctt acggctggct gcgtacagaa actggcgttc
    46861 accgcctggt gcgtaaaagc ccgtttgact ccggcggtcg tcgccacacg tcgttcagct
    46921 ctgcgtttgt ttatccggaa gttgatgatg atattgatat cgaaatcaac ccggcggatc
    46981 tgcgcattga cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat
    47041 ctgcggtgcg tattacccac atcccgaccg ggatcgtgac ccagtgccag aacgaccgtt
    47101 cccagcacaa gaacaaagat caggccatga agcagatgaa agcgaagctt tatgaactgg
    47161 agatgcagaa gaaaaatgcc gagaaacagg cgatggaaga taacaaatct gacatcggct
    47221 ggggcagcca gattcgttct tatgtccttg atgactcccg cattaaagat ctgcgcaccg
    47281 gggtagaaac ccgcaacacg caggccgtgc tggacggcag cctggatcaa tttatcgaag
    47341 caagtttgaa agcagggtta tgaggaacca acatgtctga acaacacgca cagggcgctg
    47401 acgcggtagt cgatcttaac aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc
    47461 gtgagcaggg aattgccttc ccgaacgatt tccgtcgcga tcatacctct gaccaattgc
    47521 acgcagaatt cgacggtaaa gagaacgaag aactggaagc gctgaacatc gaagtcgccg
    47581 ttgctggccg catgatgacc cgccgtatta tgggtaaagc gtctttcgtt accctgcagg
    47641 atgtgggtgg ccgcattcag ctgtacgttg cccgtgacga tctgccggaa ggcgtttaca
    47701 acgagcagtt caaaaaatgg gacctcggcg acatcctcgg cgcgaaaggt aaactgttca
    47761 aaaccaaaac cggcgaactg tctatccact gtaccgagct gcgtctgctg accaaagcac
    47821 tgcgtccgct gccggataaa ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc
    47881 gttatctgga tctcatctct aacgatgaat cccgtaacac ctttaaagta cgctcgcaga
    47941 tcctctctgg tattcgccag ttcatggtga accgcggctt tatggaagtt gaaacgccga
    48001 tgatgcaggt gatcccaggc ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc
    48061 tggatctcga catgtacctg cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg
    48121 gtggcttcga gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt atttccgtac
    48181 gtcataaccc agagttcacc atgatggaac tctacatggc ttacgcagat tacaaagatc
    48241 tgatcgagct gaccgaatcg ctgttccgta ctctggcaca ggatattctc ggtaagacgg
    48301 aagtgaccta cggcgacgtg acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc
    48361 gtgaagcgat caagaaatat cgcccggaaa ccgacatggc ggatctggat aacttcgact
    48421 ctgcgaaagc gattgctgaa tctatcggta tccacgttga gaagagctgg ggtctgggcc
    48481 gtatcgttac cgagatcttc gaagaagtgg cagaagcaca tctgattcag ccgaccttca
    48541 ttactgaata tccggcagaa gtttctccgc tggcgcgtcg taacgacatt aacccggaaa
    48601 tcacagaccg ctttgagttc ttcatcggtg gtcgtgaaat cggtaacggc tttagcgagc
    48661 tgaatgacgc ggaagatcag gcgcaacgct tcctggatca ggttgccgcg aaagacgcag
    48721 gtgacgacga agcgatgttc tacgatgaag attacgttac cgcactggaa catggcttac
    48781 cgccgacagc aggtctgggg attggcatcg accgtatggt aatgctgttc accaacagcc
    48841 ataccatccg cgacgttatt ctgttcccgg cgatgcgtcc ggtgaaataa gcattacgtt
    48901 atgctcacaa ccccggcaaa tgtcggggtt ttttatttaa gctgggtaaa tgcagataat
    48961 cgtattctgg cttcgcgatt tgtcgcctgc atcaccatcc acggactgaa cgcccacggc
    49021 gtggcatcaa taccgcgtaa tacatcagct aaatcacacc attgataatc catcacttca
    49081 tcatcgttga tctgtaacgc actggtggtg cgtgcggcaa ataccggaca cacttcattt
    49141 tccacaatgc cactcggatc ggtggcgcgg tagcgaaagt caggatagat agattcagga
    49201 ggcgtaattt ccacgccaag ctcataacgg caacggcgga tcaccgcgtc ttcgctgctt
    49261 tctcccagtt gtgggtgccc acaaaccgag ttagtccaca cgccaggcca cgcttttttg
    49321 ctcagtgcgc ggcgggtaac taataattgc cccttggcat taaacagcca actggagaac
    49381 gcgagatgta agagggtgtc tgccgtgtgt gcggcatact tttccagcgt acccgtggga
    49441 actccctgtg cattcaataa aatgacgtgt tccgtttgca taatttctca catgtaattc
    49501 tgatcactca tcattataga taattgaaga gtgaaatgat taaccaagag agcgtttatc
    49561 gacaaccgcc cgttttgagc ggatgccgac aggtaaaaac catttaaaaa agtagcatca
    49621 tatatttgaa gcgactttca atggccattg caacacagtc agaaatgaca ggaatagaga
    49681 gagtaaaaag caacaacaga ctgaatatag cgcgcattaa aaaattcata ttattctcca
    49741 tgctcatttt tcttcagctt gcgcatcaga agccacatac gtactgtcct gacttttaaa
    49801 tctttatcag agacggattc cttcaccagg tcagcatgat gctgatagcc attaaagaag
    49861 acgttcagca caacggcgct aagggttgcg agcataatgc cgctatgtag cagcggttgc
    49921 agtacggccg gtaacttaga aaagaaatcg tgagagagcg tcggagtcat gccaacgccg
    49981 agactgatcg ccacaatata gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga
    50041 attcgaatcc ctgtcgccag taccatgccg aacatcacta gaccagcgcc gcccagcaca
    50101 aattgcggaa tggaggctac cagcaccgcc atttttggca ccatgccaaa caggattaat
    50161 ataattcccg aagaaataca cacccagcgg ctatgaacgc gcatcacgct aaccaggcca
    50221 acgttttgag aaaatgacgt gtgggggaag ctgttaaacg tgccgcctat cattgtccct
    50281 acgccatcga cacgcagccc gcgaataata tcgtgtgaag agagtttacg accgactatt
    50341 tcacccagtg ccaggaacat ccccatcgac tcgataaaca cgatgattaa cacggcagtc
    50401 atggtcagaa tggaaacggg atcgaaaatc ggcataccaa acgacatcgg ggtgacaatc
    50461 gcaaaccacg aggcatcatg tagcccggat aaattgactt cattcatcat ccacgaaagt
    50521 aaaaagccaa atacaatccc cagtaatacg gcgacgttgg acataaatcc tttcgcatag
    50581 cgagtaatga gcaagataaa aattaagacg gcaaaggaga tacctaaata aacgggatta
    50641 ccatattgcg gattcccttt acctccagcg gcccagtcaa tacccacctg aatgatgcta
    50701 agcccaatag aagtaataac cacaccggta accagtggcg ggaataaagg catcaagcga
    50761 ccaataagtg gcgctaataa tgtggtgata aaacccgcgg cgatagtggc accaaatatc
    50821 cccagcaggc cgatatccgg gttcatccca atggctatca ttggtgttac agcagcaaag
    50881 gtcaccgaca taatcaccgg caggcggatc cccataaagc ggccgatacc gatacattgc
    50941 aataatgtga cgatcccgca gcaaaagaga tccgagctaa tgagcatcgc aatagcatct
    51001 tttgagaggc ccagtcggtc accaatcata agaggaacag cgactgcacc tgcgtacatg
    51061 acaaggacgt gttgcagacc gagtatgatc agctttcctg gtgataatat gcggtcaacc
    51121 tcatcagttg ggcggtcttg cccagaagat gagggaagtt gggaatctat ggcgctcatc
    51181 agtgtttctc ctttcgcccg ccagaataaa aagcaaccgt ttacgttaat gaaactgtct
    51241 aaacacatgc tcgtggagtg ccagtttttg cgaggaaggg gagttagaga caggaattgc
    51301 ggaggcaatc acataatcgt gcttaaacca cgaaaatgac aagagaacct gacaatggaa
    51361 ttatctgtaa ttttctcata ttgatatttc attaatcatt ctggcatgtc gcactctcgc
    51421 atttaatcgt ttttatctgg atagcgctct tttgatcggc aatgttgaaa taatgaaagc
    51481 ggcatagatt gaggggcaat aatttgaact cagtcgcaaa attataatta aaacggtcat
    51541 gctacctaat atatgcccgt ttattgcatc gcgaaatttg agaagacata actcattaaa
    51601 tgaaagggat gcaaatgaat aagtttatcg ctgctgaagc tgcggaatgt ataggctgtc
    51661 atgcttgtga aattgcctgt gcggtggcac acaatcaaga aaactggccg ctgagtcaca
    51721 gtgactttcg accgcgtatc cacgttgtag ggaaaggcca ggctgcgaat ccggtggcct
    51781 gccatcactg caacaatgcc ccttgtgtta cggcttgtcc ggttaatgct ctgactttcc
    51841 agtccgatag cgtacaactg gacgagcaaa aatgtattgg ctgtaaaaga tgcgcaatcg
    51901 cttgcccctt tggcgtcgtt gagatggtcg atacgattgc gcagaaatgc gacctttgta
    51961 accagcgcag ttccggcacg caagcctgta ttgaagtctg cccaacacag gcgttacgac
    52021 tgatggacga taaagggtta cagcggataa aggtggcccg ccagcgcaaa acggcagcag
    52081 gaaaagcgtc atcagacgct cagccatctc gcagtgcagc gttgctcccc gttaactcgc
    52141 gtaaaggcgc agataaaatt tcagcgagtg aacggaaaac ccactttggc gaaatctatt
    52201 gcgggctgga tccactgcaa gcgacttatg agagtgaccg ctgtgtttat tgtgccgaaa
    52261 aagctaactg caactggcat tgcccgctgc ataacgctat tccggattac atccgtctgg
    52321 tacaggaagg aaagattatt gaagcggcag aactttgcca ccagaccagt tccttaccag
    52381 aaatctgcgg cagggtatgc ccacaggacc gtctttgtga aggcgcatgt actttgaaag
    52441 atcactcagg cgcagtctct atcggtaatc tggaacgcta catcaccgat accgcgctgg
    52501 cgatgggctg gcgtcccgat gtcagcaaag ttgttccccg tatcgaaaaa gtggcggtga
    52561 ttggcgctgg gccagcaggg ttagggtgtg ctgatattct ggcgcgcgcg ggagttcagg
    52621 ttgatgtctt tgagcgccat ccagaaattg gcggtatgct gacttttggc attcctcctt
    52681 tcaaactcga taaaacggta ttaagccagc ggcgagagat attcaccgca atgggaatcg
    52741 actttcatct taactgtgaa attggccgcg atattacctt tagcgattta acttctgaat
    52801 atgatgcagt tttcatcggc gtggggactt acgggatgat gcgagcagat ctgccgcatg
    52861 aagatgcgcc cggtgtcatt caggctctac cgttcctgac tgcccatacc cgccagctca
    52921 tgggattgcc ggagtctgaa gagtatccgc tgacggacgt ggaaggtaag cgagtcgtgg
    52981 tattgggcgg tggcgatacg acaatggatt gtttgcggac ttccattcgc ctcaatgctg
    53041 ccagcgtgac ctgcgcgtat cgtcgtgatg aagtcagtat gccgggctcg cgcaaagagg
    53101 tggtcaatgc gcgcgaggaa ggtgtcgagt ttcagttcaa tgttcagccg caatatatcg
    53161 cttgtgacga agatgggcgt ttaactgcag tgggcctgat tcgtaccgcc atgggggagc
    53221 aggggccgga tggtcgccgt cgtcctcgtc cggtagcggg ttcagagttt gaattgcccg
    53281 ccgatgttct gattatggcc tttggtttcc aggcgcatgc catgccgtgg ttgcagggca
    53341 gcggaattaa actcgataaa tggggcctga ttcaaaccgg tgacgtcggg tatttaccta
    53401 cccagacgca tctgaaaaaa gtctttgctg gtggtgatgc agttcatggc gcggatctgg
    53461 ttgtcactgc catggccgca ggaaggcagg cggcgcgcga tatgttaact ctgtttgata
    53521 cgaaggcatc gtgatgaaat cgttaattat cgttaatccg gctgactgta ttggctgccg
    53581 tacttgtgaa gtagcctgtg tggtcgctca cccttcagaa caggagttga atgccgatgt
    53641 ctttttaccc cggctgaagg tacagcgact ggacatcatc agtgcgccgg tgatgtgcca
    53701 tcagtgtgaa aacgctcctt gtgttggcgc ttgccccgtg caggcgctga cgatgggcga
    53761 gcaggtggtg caggccaatt ctgcccgttg tattggctgt cagagctgcg tcagtgcgtg
    53821 tccgtttggg atgatcacca ttcagtcgtt gccgggggat acccggcaac aaatcgtgaa
    53881 atgcgatctc tgtgaacagc gggaagaggg gccagcctgc gttgaatctt gcccgacaca
    53941 ggcgctgcag ttgcttactg aaagagaact caggcgactc cgccagcagc gtattgttgc
    54001 cagcggtgag aatcctctct gaaagaaatc tccgcgctga aaggcgcgga gaatctatta
    54061 gattgcccaa ccacccgcgt aaaatgcgac cagtgcggca gtaatgatca ccgtaccaat
    54121 attcagcttt tgccattcgc gtgcaaagac gcggcctacg accagtgtca caaagcccag
    54181 cataataccg gtaacgatat tacaagtcag aacgatgaac acggcacaca ccaggccagc
    54241 catagcgtca ataaaatcat tgaaatccag cttcgagacg ttacttaaca tcagcaaacc
    54301 cacgtacatc agagcggggg cagtggcgta accagggatc aaaaatgaca gcggtgataa
    54361 aaacagaatt aacaggaata acgcccccac tacggttgcg gtcaaccctg ttttaccccc
    54421 ggcggcggtt cctgccgctg attcgatata aaccgctgcg ggcgctgcgc ccaccaggcc
    54481 ggagaatatt gaacttactg agtcactggt cagggctttg ccgccgttga tgatctggtt
    54541 gtctttatcc aacaaattcg cctgaccggc gacagcacgg atggtgccag tagcgtcgaa
    54601 cactgcggtc atcaccaatg ccagtacact cggaagtaca gttggctgga gtgcgcccat
    54661 aatatcgagg ctgaaaatca gagacttacc atcttcgcca gtcaggcttg gcatcgccac
    54721 cagaccgtgg tatttcaccg ctgggtcaaa gattaagccg atgatcgaaa ttgcaataat
    54781 caccaacaag atcccgccgg gtacgcgaca cttctccaga ccgaagatga ccgccagccc
    54841 cagtaagctc atcatcaccg ggaaggaggt aaacgcaccg agcgccactg gcaagccttc
    54901 aatcgggttt ttgataacca tacccacacc gttagcagca atcagcagca gaaacagccc
    54961 gataccgata cctgtaccgt gagcgatacc catcggcaaa ttacgtaaga tccaggtacg
    55021 cacaccggtt acggaaatgg cggtgaagat gacgcccatc agaaataccg cgcccagtgc
    55081 gacaggaacg ctaatttgtt gcccgagtac cagactgaat gcggtaaacg ccgtcaagga
    55141 aatcgcgcaa ccaatcgcca ttggcaaatt agcccataat cccatcagca acgagccgaa
    55201 gcccgcgacc agacaggtgg caacaaacac agctgcggga ggaaaacctg ctttgcccag
    55261 cattcccgga acgacgataa cggaataaac catggccaga aaggtcgtta agccagccag
    55321 tacttcctga cgaacggtac tgccacgagc ggtaatttta aaataattat ccagcgcgcc
    55381 ctgtggcttt ggtgcgtccg gtgtttgtag gatgtctcca gacataatga tgtcctcacg
    55441 aggatgttaa agttttatta ttagttgcgt tcgtacccca gacgaccatc aacgtaggtg
    55501 cggtagatcg aacggtcatc gcccaacgtc atcatcacga acaatttgtc gactaaagaa
    55561 acagagttgt catagcgcag ctgctgtagc ggagtggcgg tgggttccat caccacgaaa
    55621 tcagcctctt tgctaggtaa aaagttgcca atcaaatcgt caaggcccag agatttcgct
    55681 ccgccgagcg tggccaggta aaacgcttca tatgccgaga ggcgatagcc ttgtaattgc
    55741 aatactttgt aggcttcgtt cagcgtttgc agcatgttga aagtggttcc ggcaccgata
    55801 tccgttccca tgcccacttt aactttcttc tgccatgctt ttttcaagtt gaataagccg
    55861 ctgccgaggt aaaggttgga ggtcggacag aaagcaatgc tggatttggt ttcgctgaga
    55921 cgatcccact ctttttcttc gagatggacg cagtgagcaa agacacagtt tttaccggtc
    55981 agaccgtact gatggtaaac atccagattg atcctaccca cgtaatatgg acacaggcct
    56041 aagcgaggtt ctggttttca aattgttccg gactgaggcc gccacaccaa ctgtgccgcc
    56101 gccaccgatt gtaatcacat tcgatataat taaacaccgt tgcccgcatt atttcccggc
    56161 tgataaagtg ttctccatgg atacattcca ctttcagcga atgaaagaag ctttccacgc
    56221 aggcattatc gtagcagcaa ccttttgcgc tcatacttcc acgcagatta tgccg
//
LOCUS       DADLSB010000008        55769 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.008, whole genome shotgun sequence.
ACCESSION   DADLSB010000008 DADLSB010000000
VERSION     DADLSB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.008"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..264
                     /gene="dcd"
                     /locus_tag="JN331_000492"
     CDS             <1..264
                     /gene="dcd"
                     /locus_tag="JN331_000492"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY7258723.1"
                     /translation="LDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALR
                     PGMLIGALSFEPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            286..2139
                     /gene="asmA"
                     /locus_tag="JN331_000493"
     CDS             286..2139
                     /gene="asmA"
                     /locus_tag="JN331_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY7258724.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(2591..4174)
                     /locus_tag="JN331_000494"
     CDS             complement(2591..4174)
                     /locus_tag="JN331_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7258725.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFSFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            4833..5972
                     /locus_tag="JN331_000495"
     CDS             4833..5972
                     /locus_tag="JN331_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY7258726.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            5978..6421
                     /gene="wzb"
                     /locus_tag="JN331_000496"
     CDS             5978..6421
                     /gene="wzb"
                     /locus_tag="JN331_000496"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY7258727.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            6424..8586
                     /gene="wzc"
                     /locus_tag="JN331_000497"
     CDS             6424..8586
                     /gene="wzc"
                     /locus_tag="JN331_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY7258728.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHTRPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGGSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            8678..9517
                     /gene="wcaA"
                     /locus_tag="JN331_000498"
     CDS             8678..9517
                     /gene="wcaA"
                     /locus_tag="JN331_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HAY7258729.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEETTQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            9520..10008
                     /gene="wcaB"
                     /locus_tag="JN331_000499"
     CDS             9520..10008
                     /gene="wcaB"
                     /locus_tag="JN331_000499"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY7258730.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            10005..11222
                     /gene="wcaC"
                     /locus_tag="JN331_000500"
     CDS             10005..11222
                     /gene="wcaC"
                     /locus_tag="JN331_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY7258731.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            11197..12414
                     /gene="wcaD"
                     /locus_tag="JN331_000501"
     CDS             11197..12414
                     /gene="wcaD"
                     /locus_tag="JN331_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY7258732.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEVIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFSDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            12425..13171
                     /gene="wcaE"
                     /locus_tag="JN331_000502"
     CDS             12425..13171
                     /gene="wcaE"
                     /locus_tag="JN331_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HAY7258733.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLVHLAQADDISFEWIVVDGGSN
                     DATREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            13187..13735
                     /gene="wcaF"
                     /locus_tag="JN331_000503"
     CDS             13187..13735
                     /gene="wcaF"
                     /locus_tag="JN331_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY7258734.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            13761..14882
                     /gene="gmd"
                     /locus_tag="JN331_000504"
     CDS             13761..14882
                     /gene="gmd"
                     /locus_tag="JN331_000504"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY7258735.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            14885..15853
                     /gene="fcl"
                     /locus_tag="JN331_000505"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             14885..15853
                     /gene="fcl"
                     /locus_tag="JN331_000505"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY7258736.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPASLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            15853..16332
                     /locus_tag="JN331_000506"
     CDS             15853..16332
                     /locus_tag="JN331_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY7258737.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            16329..17552
                     /gene="wcaI"
                     /locus_tag="JN331_000507"
     CDS             16329..17552
                     /gene="wcaI"
                     /locus_tag="JN331_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY7258738.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            17555..18991
                     /gene="cpsB"
                     /locus_tag="JN331_000508"
     CDS             17555..18991
                     /gene="cpsB"
                     /locus_tag="JN331_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY7258739.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLCQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            19184..20554
                     /gene="cpsG"
                     /locus_tag="JN331_000509"
     CDS             19184..20554
                     /gene="cpsG"
                     /locus_tag="JN331_000509"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY7258740.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            20609..22003
                     /gene="wcaJ"
                     /locus_tag="JN331_000510"
     CDS             20609..22003
                     /gene="wcaJ"
                     /locus_tag="JN331_000510"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY7258741.1"
                     /translation="MINLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            22005..23483
                     /gene="wzxC"
                     /locus_tag="JN331_000511"
     CDS             22005..23483
                     /gene="wzxC"
                     /locus_tag="JN331_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY7258742.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIIHYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            23663..24943
                     /gene="wcaK"
                     /locus_tag="JN331_000512"
     CDS             23663..24943
                     /gene="wcaK"
                     /locus_tag="JN331_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY7258743.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            24940..26160
                     /gene="wcaL"
                     /locus_tag="JN331_000513"
     CDS             24940..26160
                     /gene="wcaL"
                     /locus_tag="JN331_000513"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY7258744.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            26171..27565
                     /gene="wcaM"
                     /locus_tag="JN331_000514"
     CDS             26171..27565
                     /gene="wcaM"
                     /locus_tag="JN331_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY7258745.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            27740..28633
                     /gene="galF"
                     /locus_tag="JN331_000515"
     CDS             27740..28633
                     /gene="galF"
                     /locus_tag="JN331_000515"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY7258746.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            29006..30091
                     /gene="rfbB"
                     /locus_tag="JN331_000516"
     CDS             29006..30091
                     /gene="rfbB"
                     /locus_tag="JN331_000516"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7258747.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHAGICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            30091..30990
                     /gene="rfbD"
                     /locus_tag="JN331_000517"
     CDS             30091..30990
                     /gene="rfbD"
                     /locus_tag="JN331_000517"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY7258748.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            31049..31927
                     /gene="rfbA"
                     /locus_tag="JN331_000518"
     CDS             31049..31927
                     /gene="rfbA"
                     /locus_tag="JN331_000518"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7258749.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            31932..32477
                     /gene="rfbC"
                     /locus_tag="JN331_000519"
     CDS             31932..32477
                     /gene="rfbC"
                     /locus_tag="JN331_000519"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY7258750.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWQVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            32493..33749
                     /gene="wzx"
                     /locus_tag="JN331_000520"
     CDS             32493..33749
                     /gene="wzx"
                     /locus_tag="JN331_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY7258751.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            33779..34669
                     /locus_tag="JN331_000521"
     CDS             33779..34669
                     /locus_tag="JN331_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY7258752.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNSTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGDGRYNA"
     gene            34698..35567
                     /locus_tag="JN331_000522"
     CDS             34698..35567
                     /locus_tag="JN331_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7258753.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            35587..36735
                     /gene="wzy"
                     /locus_tag="JN331_000523"
     CDS             35587..36735
                     /gene="wzy"
                     /locus_tag="JN331_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY7258754.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSAKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            36739..37113
                     /locus_tag="JN331_000524"
     CDS             36739..37113
                     /locus_tag="JN331_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HAY7258755.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            37176..38102
                     /locus_tag="JN331_000525"
                     /pseudo
     CDS             37176..38102
                     /locus_tag="JN331_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067396.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            38287..38886
                     /locus_tag="JN331_000526"
     CDS             38287..38886
                     /locus_tag="JN331_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258756.1"
                     /translation="MSLSILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFAVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYLGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            38987..39538
                     /locus_tag="JN331_000527"
     CDS             38987..39538
                     /locus_tag="JN331_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258757.1"
                     /translation="MIAKKRNIQTFLIFSLATILGGIYWYVRSFWISGDPFSPAGGNI
                     FGHYLWNEIDLQVQTAEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKIL
                     WVFFSIVLTYVLFWFSVTQGDRYLSPIYPLSVLLVAISIASLIKDINITNYKLGRYFI
                     LVCIIFFRGDRCILNSNIFILNT"
     gene            39535..39894
                     /locus_tag="JN331_000528"
     CDS             39535..39894
                     /locus_tag="JN331_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258758.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIFISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            40003..41409
                     /gene="gndA"
                     /locus_tag="JN331_000529"
     CDS             40003..41409
                     /gene="gndA"
                     /locus_tag="JN331_000529"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY7258759.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            41659..42825
                     /gene="ugd"
                     /locus_tag="JN331_000530"
     CDS             41659..42825
                     /gene="ugd"
                     /locus_tag="JN331_000530"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY7258760.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            42971..43948
                     /gene="wzzB"
                     /locus_tag="JN331_000531"
     CDS             42971..43948
                     /gene="wzzB"
                     /locus_tag="JN331_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY7258761.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(44134..44745)
                     /locus_tag="JN331_000532"
     CDS             complement(44134..44745)
                     /locus_tag="JN331_000532"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY7258762.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANSIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(44739..45515)
                     /gene="hisF"
                     /locus_tag="JN331_000533"
     CDS             complement(44739..45515)
                     /gene="hisF"
                     /locus_tag="JN331_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY7258763.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            complement(45497..46234)
                     /gene="hisA"
                     /locus_tag="JN331_000534"
     CDS             complement(45497..46234)
                     /gene="hisA"
                     /locus_tag="JN331_000534"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY7258764.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(46234..46824)
                     /gene="hisH"
                     /locus_tag="JN331_000535"
     CDS             complement(46234..46824)
                     /gene="hisH"
                     /locus_tag="JN331_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY7258765.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRHSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            complement(46824..47891)
                     /gene="hisB"
                     /locus_tag="JN331_000536"
     CDS             complement(46824..47891)
                     /gene="hisB"
                     /locus_tag="JN331_000536"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY7258766.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            complement(47891..48961)
                     /gene="hisC"
                     /locus_tag="JN331_000537"
     CDS             complement(47891..48961)
                     /gene="hisC"
                     /locus_tag="JN331_000537"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY7258767.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRPLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            complement(48958..50262)
                     /gene="hisD"
                     /locus_tag="JN331_000538"
     CDS             complement(48958..50262)
                     /gene="hisD"
                     /locus_tag="JN331_000538"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY7258768.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLIRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYVAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            complement(50268..51167)
                     /gene="hisG"
                     /locus_tag="JN331_000539"
     CDS             complement(50268..51167)
                     /gene="hisG"
                     /locus_tag="JN331_000539"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY7258769.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            complement(51313..51363)
                     /gene="hisL"
                     /locus_tag="JN331_000540"
     CDS             complement(51313..51363)
                     /gene="hisL"
                     /locus_tag="JN331_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY7258770.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            51646..51897
                     /gene="yefM"
                     /locus_tag="JN331_000541"
     CDS             51646..51897
                     /gene="yefM"
                     /locus_tag="JN331_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY7258771.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            51894..52148
                     /gene="yoeB"
                     /locus_tag="JN331_000542"
     CDS             51894..52148
                     /gene="yoeB"
                     /locus_tag="JN331_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY7258772.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            52231..53055
                     /locus_tag="JN331_000543"
     CDS             52231..53055
                     /locus_tag="JN331_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7258773.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHT
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGRIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            53101..54030
                     /locus_tag="JN331_000544"
     CDS             53101..54030
                     /locus_tag="JN331_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7258774.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            54245..54307
                     /gene="yoeI"
                     /locus_tag="JN331_000545"
     CDS             54245..54307
                     /gene="yoeI"
                     /locus_tag="JN331_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY7258775.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            54297..55655
                     /gene="plaP"
                     /locus_tag="JN331_000546"
     CDS             54297..55655
                     /gene="plaP"
                     /locus_tag="JN331_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY7258776.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIVLVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWICEKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT        14193 a        12935 c        14155 g        14486 t
ORIGIN      
        1 ctggacgggc gttcctcttt ggcgcgtctg gggctgatgg tgcacgtcac cgcgcaccgt
       61 atcgatccgg gctggtctgg ttgcattgtg ctggagttct acaactccgg taagctgccg
      121 ctggcgctgc gtccgggcat gttaattggt gcgctgagct ttgagccgct ttccggcccg
      181 gcggcgcgac cttacaaccg ccgtgaagat gcgaaatatc gcaaccagca gggcgcggta
      241 gccagccgaa tcgataaaga ctaattcagg cccattgagg ataccatgag acgatttctg
      301 acgacgctga tgatactcct ggtcgtgctg gtggccgggt tatctgcgtt agtgttgctg
      361 gtgaatccga atgatttccg cgactatatg gtcaagcaag ttgctgcacg tagcggttat
      421 caattgcagc tcgacgggcc actgcgttgg cacgtctggc cgcagcttag tatcctctcc
      481 gggcgaatgt ctctcaccgc ccagggcgca agccagccac tggttcgcgc cgacaacatg
      541 cgtctggacg tggcgctttt accactactg agtcatcaac tgagcgttaa gcaggtgatg
      601 ctaaaagggg cagtgatcca actgacgccg cagacggaag cggtgcgcag tgaagacgct
      661 ccggttgcac cgcgcgacaa taccttgccg gatctgtcag acgatcgcgg atggtcgttt
      721 gatatatcca gtcttaaggt ggcggacagc gttctggtgt tccagcatga agatgacgag
      781 caggtgacaa tccgcaatat ccgcctgcaa atggaacaag atccccaaca tcgtggctca
      841 tttgagttct ccgggcgggt taatcgcgat cagcgcgatc tcacgatatc ccttaacggt
      901 acggtagatg cttctgatta tccgcatgat ttaacagcgg ctattgaaca aattaactgg
      961 cagttgcagg gtgccgattt accaaaacaa ggtattcagg ggcaggggag tttccagtcc
     1021 cagtggcagg agtcacataa acgcctttca tttaaccaaa ttagtttgac cgccaatgac
     1081 agtacgctga gcgggcaagc acaggtcacg ctgacagaga aaccggaatg gcagctgaga
     1141 ctgcaattcc cgcaactgaa tcttgacaac ctcatcccgc ttaatgaaac cgcgaatggt
     1201 gaaaacggtg ccgcgcagca ggggcagagc caatcaacgt tgccgcgccc ggtcatttct
     1261 tcgcgtattg atgaaccggc ctatcaggga ctgcaaggct ttacagctga tattttgttg
     1321 caggccagta acgtgcgctg gcgcggaatg aattttacag atgttgccac gcaaatgacc
     1381 aacaagtcgg gtttgctgga aattactcaa ctgcagggca agcttaacgg tggaaaggtt
     1441 tcactgccgg gcacgctgga cgcgacatca ataaatccgc ggataaactt ccagccacgg
     1501 ctggagaacg ttgagattgg taccattctg aaggcgttta actatccgat ttcgttgacc
     1561 ggaaaaatgt cactggctgg tgatttctcc ggtgctgaca tagatgccga cgcattccgc
     1621 cacaactggc aagggcaggc gcatgttgag atgaccgaca cacgtatgga agggatgaac
     1681 ttccagcaga tgattcagca agcggtagag cgtaatggcg gtgatgtgaa ggccgctgaa
     1741 aacttcgata acgtgacgcg gcttgaccgc tttaccaccg atttgacgtt gaaggatggc
     1801 gtcgtgacgt taaacgacat gcaaggtcaa tcgcctgtgc tggcgctgac aggggaaggc
     1861 atgttgaatc tggcagatca aacctgcgac acccagtttg atattcgggt cgtgggtggc
     1921 tggaacgggg aaagcaaact gattgatttc ctcaaagaaa cgccagtacc gctgcgggtt
     1981 tatggcaact ggcagcaact caattacagc ctgcaagtgg atcagttact gcgcaaacat
     2041 ctacaggacg aagcgaaacg tcgcctgaat gactgggccg agcggaataa agattcccgc
     2101 aatggcaaag atgtgaagaa gttgctggag aagatgtagg ttaccaggtg acctgtcctg
     2161 taggcctgat aagacgcgta aagcgtcgca tcaggcgttc gcggcggcac tattatattc
     2221 tgaatatgta agatcgaagg ctgttccgtt cacttgatgt tcctcgctac tatggaacgc
     2281 tcaagccggt gaacgtgcca gggtggctca atcgccacca ccctggcaac ccgggctccc
     2341 ggcggtaaat cgccgctggc gcggttccct catcttattc ctttcggcta tcgggtacgg
     2401 gcgcgaggta acatccctgt aaaacgcgcc ctcagcccac atccatgtgg gctgccccag
     2461 cctacaggaa acgattcggc gatttacagc cggacccaca ccaccgctgt gatgtccgtt
     2521 taccacagac aacgcttcat tgctgaagtt ataaggcgtt acgcagcatt gggtaaaatg
     2581 gccgatacca tcacacctcg tactccatct cgccatcctt acgcagcggt ataatctgca
     2641 ccttctgcac gcgatggctt tccacctgca acgttttaag caaataatcc cccacctgaa
     2701 cttcttcgcc aggttttgga atacgctgca aatactccat caacagcccg gcaatggtgt
     2761 gatattcacg tttttcatcc agcggcagcg gcacatattg caccagatcc tccagcggca
     2821 tatgaccatt cgccgtccag gaaccgtccg cattcttctg aatatcatgg cgggcgtcga
     2881 tctcttccac ttcgttcggt aagttaccgg caatggtttc agtgacgtca cttaatgtca
     2941 caatcccttc caccgagcca aactcatcca ccacaaaagc aaagtgcgtg cgggcattac
     3001 ggaactgctc cagggctggt aacaacggca aggtttccgg gaacaccagc ggctggcgaa
     3061 tcaacacccg caggttgagc ggttcgccgc gcagtgactg ttgtagcagg tcgataacgt
     3121 gaacaacacc gagcaaatct tctgcgtcat caccgtcggt aaccaccagc cgcgtatgct
     3181 gatttcgctc cagcaactgg cggatttctt cttccggcgc gttgagatcg atatgctcaa
     3241 tatcgtggcg cgacgtcata atgctgctga cggtacgctg gttaagatta agtacccgct
     3301 caatcatccg ccgttcctgc ggattaaaga tctgctggtt tccatgatcc accagcatgg
     3361 acgcggtttc ggcgtccagt tccgcatctt ctttttgccc gctaagcagg cgcattaccg
     3421 cttcggtagt ccgctggcgt agcgtctgat tagcggaaag aaaacgtcgc cggttaaaga
     3481 tagccaactg attgagtgcc tcgatcatca ctgagaaacc aatggcagcg tacaggtagc
     3541 ctttcggaat gacgaagctg aaaccttctg ccaccaggct aaagccaatc atcaacagga
     3601 agcttaagca gagaataacg atggtcgggt gactgttaac aaattgcgtt aacggcttgc
     3661 tggccatcaa catcaggctg atagcgataa ccacggcggc catcatgacc agtaaatggt
     3721 cgaccatccc gacggcggta atcactgagt cgagtgagaa aatggcgtcc agtaccacaa
     3781 tttgcgtcac cacgccccag aacttcgcac ctttgcgttg tgtgggatta ttgctgtctt
     3841 tcccttccag ccgttcgttc agctccatcg tggctttgaa caacaggaag aaaccaccaa
     3901 acagcatgat taagtcgcgg gcgctaaagg taaacgagcg gaagctgaac agcggttgag
     3961 tcagggtgac cagccaggag attgacgcca gcagtaacag gcgcattaac atcgccagca
     4021 gcagcccggt aacccgtgcg cggtcgcgct gcttcggcgg tagtttttcg gcgaggatgg
     4081 caataaagac caggttatca atgccgagga ccagttcgat cacaatcagc gtgattaacc
     4141 cggcccagat agacggatcg gcaatccatt ccatagtaag tacaaaacct attcgttgta
     4201 tgacaaatgt cacaattcga tcatggataa atcggggcaa aaatgcaatg ttgcagcact
     4261 atttatctca tattatttga gggtaataac ctcacattat ccctgaatta aaagcggtaa
     4321 taataaagca ttatttaaga aatatcgcat atggagacgc aatattaaat attgtcaacc
     4381 taaagaaact cctaaaaacc atattgaatg acacttaata taattcttaa aaatagccaa
     4441 ttgccgaatt gttaccttgc ctgctattcc gttagctgta acacttcctc ctgcattatt
     4501 ggagagccaa tattcaatta ccataatgtc accttatatt cggaagttaa tttccgcaat
     4561 aaccctttct catcaacgat tgtatgcaga gagggtttga aaaattaagc tcaatcagtg
     4621 tattggtagc taaaaagcca ggggcggtag cgtgtctgga tgcctgaaag accagtctga
     4681 attattctgt caatagcctg cggatgaaat caattttttt aggactgatg ccagttaaat
     4741 ttttattcaa ttaactgcaa ggtaaatgtg catggcgaca ttgctattaa tagtgcacag
     4801 gataattact ctgccaaagt gataaataaa caatgatgaa atccaaaatg aaattgatgc
     4861 cattattggt gtcagtaacc ttgataagcg gttgcacagt acttccgggc agcaatatgt
     4921 cgacgatggg caaagacgtc atcaaacagc aggacgctga tttcgatctc gacaaaatgg
     4981 tgaatgttta tccgctgacc ccgcgcctga ttgaccaatt acgcccacgc ccgaatgtag
     5041 cgcgccccaa tatgacgctg gaaagcgaga tcgcgaatta ccagtatcgc gtcgggccgg
     5101 gggatgttct taatgtcacc gtctgggatc acccggaact caccacgcca gctggtcagt
     5161 accgcagctc cagcgacacc ggcaactggg tacagcctga cggcactatg ttttacccgt
     5221 atatcggcaa ggttcacgta gtcgggaaaa cgctcgctga aatccgcagt gatattaccg
     5281 ggcgcttagc gacgtacatc gctgacccgc aggtggacgt taatatcgcc gccttccgct
     5341 cacaaaaggc ctatatctca ggtcaggtga ataaatccgg tcaacaggcg atcaccaacg
     5401 taccactgac cattctcgac gccatcaacg ccgcaggtgg cctgaccgac accgctgact
     5461 ggcgcaacgt ggtgctaaca cacaatggtc gtgaagaacg catttcttta caggcgctga
     5521 tgcaaaacgg cgacctcaat cagaaccgac tgctttatcc cggcgatatt ctctacgtgc
     5581 cgcgtaatga tgatctgaaa gtatttgtga tgggtgaagt gaagaaacag agcaccctga
     5641 aaatggactt tagcggcatg accctgactg aagctctggg caatgccgaa ggtatcgaca
     5701 tgaccacctc caacgccagc ggcatctttg tcattcgtcc gctgaaaggc gagggcgggc
     5761 gtaacggcaa gattgccaat atctaccagc tggatatgtc cgatgcgacg tcgctggtga
     5821 tggcgacaga attccgcctg caaccttatg acgtggtgta tgtcaccacc gccccggttt
     5881 cccgctggaa ccgtctgatc aatcagttgc tgccaactat tagcggtgtc cgttacatga
     5941 cggatacagc cagcgacatt cataactggt aatcgtcatg tttaacaaca tcttagttgt
     6001 ctgtgtcggc aatatttgcc gttccccgat ggcggaacgc ttactgcaac gttatcaccc
     6061 ggaactgaaa gtggagtccg ctggactcgg cgcgctggtc ggtaagggcg ctgatcctac
     6121 cgctatcagc gtcgccgcag aacatcaact gtctctggaa ggccactgtg cacgacaaat
     6181 cagccgccgc ctgtgtcgca actatgacct gattttgacc atggataagc gccatatcga
     6241 acgcttatgc gagatggcac cggagatgcg cggcaaagtg atgctgtttg gtcactggga
     6301 taacgaatgt gaaatccccg atccgtatcg caaaagccgg gaaacgtttg cagcggtgta
     6361 cacattactt gaacggtctg cccgccagtg ggcgcaggca ttgaacgcag agcaggtata
     6421 agaatgacag aaaaagtaaa acaacatgcc gctccggtaa cgggcagtga tgaaatcgat
     6481 attggtcgcc tggtcggcac cgtcattgaa gcgcgctggt gggtgattgg catcaccgcc
     6541 gtatttgccc tctgtgccgt ggtttacact ttcttcgcca cgccgattta tagtgccgac
     6601 gcactggtac aaatcgagca aaacagcggc aattcgctgg tgcaggatat cggctcggca
     6661 ttagccaaca aaccgcctgc atcggacgcc gagatccagt tgattcgttc gcgtctggtg
     6721 cttggtaaaa cggtggacga tctcgacctc gatattgcgg tgagcaaaaa cacgttcccg
     6781 attttcgggg cgggctggga tcgcctgatg gggcgtcaga acgagacggt gaaagtgact
     6841 acctttaacc gcccgaaaga gatggcggat caggtgttta cgcttaatgt gctggatgac
     6901 aaaaactaca ccctgagcag tgatggcggc tttagcgccc gtgggcaagc gggccagatg
     6961 ctgaaaaaag aaggcgtcac gctgatggtt gaagccattc acacccgccc gggcagtgag
     7021 tttaccgtca ccaaatactc cacgttgggg atgatcaacc aactgcaaaa cagcctgacg
     7081 gtaacggaga acggcaaaga cgcaggcgta ctgagcctga cttataccgg tgaagatcgc
     7141 gaacagatcc gcgacattct taacagcatc gctcgtaact atcaggaaca aaatattgag
     7201 cgcaaatcgg cggaagcgtc gaaaagcctc gctttcctcg cgcaacagtt accggaagta
     7261 cgtagccgcc tcgatgttgc cgaaaacaaa ctgaatgcct tccgtcagga taaagattct
     7321 gttgatctgc cgcttgaagc gaaagcggtg ctcgattcga tggtgaacat cgacgcgcag
     7381 ttgaacgaac tgacctttaa agaggcggaa atctccaagc tgtacaccaa agttcacccg
     7441 gcgtaccgca cgctgctgga gaaacgtcag gcgctggaag aagagaaagc caaacttaac
     7501 ggtcgcgtaa cggcgatgcc gaaaacccag caggaaattg tccgtctgac ccgcgatgtc
     7561 gagtctggtc agcaggtcta tatgcaactg ctgaataaag agcaggagct gaaaatcacc
     7621 gaagccagca ccgtcggcga tgtgcgcatt attgacccgg caatcaccca gcctggcgtg
     7681 ctaaaaccga agaaagggct gattatcctc ggcgcgatta tccttggcct gatgctctct
     7741 atcgtgggtg tgctgctgcg ctcgttgttt aatcgcggca tcgaaagccc gcaagtgctg
     7801 gaagaacacg gaatcagcgt ctatgccagc atcccgctgt cggagtggca gaaagcgcgc
     7861 gatagcgtca aaaccatcaa aggggttaaa cgctataaac agagccagct actggcagtg
     7921 gggaacccaa ccgatctggc gattgaagcc atccgtagtc tgcgtaccag tctgcacttc
     7981 gcgatgatgc aggcgcagaa caatgtgttg atgatgaccg ggggtagccc gtcaatcggt
     8041 aaaacctttg tctgcgccaa cctggcggcg gtgatcagcc agaccaataa acgcgtgttg
     8101 ttgatcgact gcgatatgcg caaaggctac acccacgagc tgttgggcac caataatgtc
     8161 aatgggctgt cggagattct gattggtcag ggcgatatta ctaccgctgc taaaccgacc
     8221 tctattgcca aatttgacct gatcccgcgc ggtcaggtgc cgccaaatcc ttctgaactg
     8281 ttaatgagcg aacgctttgc cgaactggtg aactgggcga gtaaaaacta tgacctggtg
     8341 ttgattgata cgccgccgat tctggcagtg accgatgcgg caattgttgg tcgtcatgtc
     8401 ggaaccacgt taatggtggc gcgttatgcg gtcaacacat tgaaagaagt ggaaaccagt
     8461 ctgagccgct ttgagcaaaa cggtattccg gtgaaagggg tgattctgaa ctccatcttc
     8521 cgccgcgcca gcgcgtatca ggattatggc tattacgaat acgaatataa gtcggatgcg
     8581 aaataacgac gcctgcattc gcaccgcccc gtaggccgga taaggcgctc cgccgcatcc
     8641 ggcaagcaaa ccagctcata agccgggaga acaacctatg aaagacaatc cgctgatctc
     8701 aatctatatg ccaacctgga accgccaaca actggcgatt cgggcgataa aatcggtcct
     8761 gcgccaggac tacagcaact gggagatgat catcgtggat gattgctcca cctcctggga
     8821 acaactgcaa cagtacgtca ctgccctcaa cgatccgcgt atcacttaca tccacaacga
     8881 cattaacagc ggggcgtgcg cggtgcgtaa ccaggcgatt atgctggcgc aaggggaata
     8941 catcaccggg atcgatgatg atgatgaatg gacgcccaac cgcctgagcg tcttcctcgc
     9001 ccataaacag caactggtca cacacgcctt tttgtacgct aacgactacg tttgccaggg
     9061 agaagtttat tcccaaccgg cgagcttacc actgtatccc aaatcgccgt actcacgccg
     9121 cctgttctat aaacgcaata tcattggcaa tcaggtcttt acctgggcat ggcgtttcaa
     9181 agagtgtttg ttcgataccg aactgaaagc agcacaggat tacgacatat tcctgcggat
     9241 ggtggtggag tacggcgaac cgtggaaagt ggaagagacg acgcagatcc tgcatatcaa
     9301 tcacggcgaa atgcagatca cctcgtcacc gaaaaaattc tccggatact tccactttta
     9361 ccgcaagcac aaagacaaat tcgaccgcgc cagtaaaaaa tatcagctgt ttaccctcta
     9421 ccagatccgc aataaacgta tgacctggcg caccttgttg acgctgctgt cggtgcgtaa
     9481 cggcaaacgt ctggctgatg gtattcgggg gcgctaaaga tgctggaaga tctgcgcgcc
     9541 aacagctgga gtttacgccc gtgctgcatg gtacttgcct atcgcgtcgc tcatttttgt
     9601 tcggtgtggc gcaaaaagaa cgtcctcaac aatctgtggg cggccccgct gctggtgctg
     9661 tatcgcatta tcaccgaatg ctttttcggt tatgaaatcc aggccgccgc gaccattggc
     9721 cgccgcttta ctatccatca cggttacgcc gtagtgatca ataaaaacgt ggtggcgggg
     9781 gatgatttca ccattcgcca cggcgtcact atcggcaatc ggggtgccga taacatggca
     9841 tgtccacaca ttggcaacgg cgtcgaactc ggtgccaacg tcattattct tggtgatatc
     9901 acgcttggta acaacgtcac cgtgggtgcg ggcagcgtgg tgctcgactc tgtcccggac
     9961 aacgcgctgg tggtgggaga aaaagcgcga gtgaaggtaa ttaaatgaat attctgcaat
    10021 ttaatgtgcg actggcggaa ggcggggcag caggtgtggc gttagatctc caccagcgtg
    10081 cgctgcaaca ggggctggcg tcacattttg tctacggcta cggcaaaggc ggcaaagaga
    10141 gcgtcagcca tcagaactat ccgcaggtca tcaaacatac gccgcggatg accgcgatgg
    10201 cgaacattgc gttgtttcgt ctgtttaatc gcgatctgtt cggcaatttc aatgagttat
    10261 atcgcaccat tactcgcaca ccgggtccgg tggtcctgca ttttcatgtg ctgcacagct
    10321 actggttgaa tcttaacagc gtggtgcgct tttgcgaaaa agtgaaaaac cataaaccgg
    10381 atgtcaccct ggtctggacg ctgcacgacc actggagtgt aaccggacgc tgcgccttta
    10441 ccgacggttg cgaaggctgg aaaacgggct gccagaaatg cccgacctta aataactatc
    10501 cgccggtgaa gattgatcgc gcgcaccagc tggtggcggg caaacgccag ttattccgcg
    10561 agatgctggc gctgggctgt cagtttattt cccccagcca gcatgtggct gacgctttca
    10621 atagtctgta cgggccaggg cgttgccgga ttatcaataa tggcattgat atggcaaccg
    10681 aagcgattct ggcagatctg cctccggtgc gcgaaactca gggcaagccg aaaatcgcgg
    10741 tggtggcgca tgacctgcgt tacgacggca aaactaacca gcaactggtg cgtgagatga
    10801 tggcgctggg cgacaaaatc gaactgcata cctttggtaa gttctcgccg ttcaccgctg
    10861 gcaacgtggt taatcacggc tttgaaactg acaagcgcaa gttgatgagc gcgctcaatc
    10921 agatggatgc gctggtgttc agttctcgtg tcgataacta cccgctgatt ttgtgtgagg
    10981 cgctatcgat tggcgtgccg gtgattgcca cccatagcga tgcggcgcgg gaagtgctgc
    11041 aaaaatccgg tggtaaaacc gtcagtgaag aagaggtgct gcaactggta cagttaagca
    11101 aaccggaaat cgcgcaggcg atatttggta ccacgctggc tgagttcagc caacgcagtc
    11161 gcgccgccta cagtggacaa cagatgctgg aggagtatgt caacttctat cagaatctgt
    11221 agctacctgc tgctgccact gatctacctg ctggttaacg tcaaaatcgc ccagcttggc
    11281 gaaagtttcc ccatcaccat cgtcactttt ttacctgtct tgttactgct gtttttagaa
    11341 cgcatcagca ttaaaaaatt gatgattgcc ttagggattg gcgcgggact cacggcgttt
    11401 aactacctgt ttggtcagtc gttggatgcc ggtaaatacg tcacttcaac tatgctgttt
    11461 gtctatattg tgatcattat tggcatggtg tggagtattc gttttaaaac aatttcgcca
    11521 cacaaccatc gtaagatatt acgtttcttt tatctggtgg tcggactggt ggtggcgctg
    11581 gcggcggtgg agatggcgca aattatcctc accggtggca gcagtattat ggagtcgatt
    11641 tcgaaatatc tgatttacag caacagctat gtgctgaatt tcattaaatt cggcggcaag
    11701 cgcacgacag cactttattt cgaaccggca tttttcgctc tggcattaat ctcaatttgg
    11761 ctcagcatca aacagtttgg tatcaaaacg cctaaaacag atgctatgat tctcgcaggg
    11821 ataatattat ccggatcgtt ttcaggggtt atgaccttta tcctgtttta tttgctggag
    11881 tgggcatttc aatatctgaa taaagaggtg attaagaaaa aattaccgtt agcattgatt
    11941 tctctggctg tattcctggt tggtgtggta atcgcgtttc cttatatttc cacccgtctg
    12001 ggcgatttag gtacggaagg atcgtcatcg tattatcgta ttgtcggtcc gctggtgatg
    12061 gtcggttatt ctttgaccca tattgacggt gtagtcagat ttggctcact ttatgaatat
    12121 gttgcatcat tcggaatatt taacggtgcg gatgtcggaa aaaccataga caatggtttg
    12181 tatctgctga ttatttattt ttcctggttc gcggtgtttt tatcgctgtg gtacatgggg
    12241 aaagtgataa aaatgatgat caacgctttt agtgataacc gcaattttcg cgtgcaatta
    12301 tatcttttta ctccggtatc gctgtttttt accggttcga tatttagccc ggaatatgca
    12361 tttttaatcg tctgtccgtt tattttgcga aaagcgttaa atattacgag gtaagaataa
    12421 gaacatgttg cttagcataa tcactgtcgc gtttcgtaac ctcgaaggga tagtcaaaac
    12481 acatgcctcg ctggtgcatc tggcgcaggc ggacgatatc agcttcgaat ggattgttgt
    12541 cgatggcggt tccaacgacg ctacccgtga gtatctggaa aatctcaatg gtatctataa
    12601 cctacgcttt gtcagcgagc cagataacgg tatctacgac gccatgaaca agggcattgc
    12661 gatggcacaa ggcaagttcg cgttgtttct caactcgggc gatatttttc atcaggatgc
    12721 cgcaaatttt gtccgtaagt taaaaatgca aaaagataac gtgatgatca ctggcgatgc
    12781 gctgctggat tttggcgacg ggcataaaat taaacgtagc gccaaaccgg gctggtatat
    12841 ttatcacagc ctgcccgcca gccatcaggc gatatttttc ccggtatccg gcttgaaaaa
    12901 atggcgttat gacctggaat ataaagtttc ttctgattac gcgctggcag ctaaaatgta
    12961 taaagccggt tatgcattta aaaaactcaa tggcctggtg tctgaatttt ctatgggtgg
    13021 ggtatcaacc accaataata tggaattgtg tgctgacgcc aaaaaagtcc aacggcagat
    13081 attacatgta cctggcttct gggcagaatt atcctggcat ttacgccaac gtacgacagg
    13141 taagactaaa gccttatata acaaagtctg aatataagga aaaccaatgc aagatttaag
    13201 cggtttctcg gtgccgaaag ggttccgggg cggcaacgct attaaagtgc aattatggtg
    13261 ggcagtacag gcaacaatat ttgcctggtc gccacaagta ttgtatcgct ggcgggcttt
    13321 tttattacgt ttattcggag caaaaatagg aaaaaacgta gttattcgtc cgtcagtaaa
    13381 aattacctat ccgtggaaat taaccttagg tgattacgcg tgggtcggcg atgacgtcaa
    13441 tttatatacc ctcggtgaaa taaccattgg cgcacattcg gtgatatcgc aaaaaagtta
    13501 tttatgcacc ggtagccacg accatgcaag tcaacatttc accattaacg ccacgcctat
    13561 tgtgattggc gagaaatgct ggctggcaac cgatgtcttt gttgccccag gcgtcacaat
    13621 cggcgacggc accgtcgtgg gtgcacgaag cagtgttttt aaatcgcttc cggcaaatgt
    13681 ggtttgccga gggaatcccg cagtggtgat acgcgaacgc gttgaaactg aataaattca
    13741 aaaatacaga ggaataagac atgtcaaaag tcgctctcat caccggtgta accggacaag
    13801 acggttctta cctggcagag tttctgctgg aaaaaggtta cgaggtgcat ggtattaagc
    13861 gtcgcgcatc gtcattcaac accgagcgcg tggatcacat ttatcaggat ccgcacacct
    13921 gcaacccgaa attccatctg cattatggcg acctgagtga tacctctaac ctgacgcgca
    13981 ttttgcgtga agtacagccg gatgaagtgt acaacctggg cgcaatgagc cacgttgcgg
    14041 tctcttttga gtcaccagaa tataccgctg acgtcgacgc gatgggtacg ctgcgcctgc
    14101 tggaggcgat ccgcttcctc ggtctggaaa agaaaactcg tttctatcag gcttccacct
    14161 ctgaactgta tggtctggtg caggaaattc cgcagaaaga aaccacgccg ttctacccgc
    14221 gatctccgta tgcggtcgcc aaactgtacg cctactggat caccgttaac taccgcgaat
    14281 cctacggcat gtacgcttgt aacggaattc tcttcaacca tgaatccccg cgccgcggcg
    14341 aaaccttcgt tacccgcaaa atcacccgcg caatcgccaa catcgcccag gggctggagt
    14401 cgtgcctgta cctcggcaat atggattccc tgcgtgactg gggccacgcc aaagactacg
    14461 taaaaatgca gtggatgatg ctgcaacagg aacagccgga agatttcgtt atcgcgaccg
    14521 gcgttcagta ctccgtacgt cagttcgtgg aaatggcggc agcacagctg ggcatcaaac
    14581 tgcgctttga aggcacgggt gttgaagaga agggcattgt ggtttccgtc accgggcatg
    14641 acgcgccggg cgttaaaccg ggtgatgtga ttatcgccgt tgatccgcgt tacttccgtc
    14701 cggcagaagt tgaaacgctg ctcggcgacc cgaccaaagc gcacgaaaaa ctgggctgga
    14761 aaccggaaat caccctcaga gagatggtgt ctgaaatggt ggctaatgac ctcgaagcgg
    14821 cgaaaaaaca ctctctgctg aaatctcacg gctacgacgt ggcgatcgcg ctggagtcat
    14881 aagcatgagt aaacaacgca tttttatcgc tggccattgt gggatggtcg gttctgccat
    14941 caagcggcag ctcgaacagc gcggtgatgt ggagctggta ttacgtaccc gcgatgaact
    15001 gaacctgctg gacagccgcg ccgtgcatga tttctttgcc agcgaacgca ttgaccaggt
    15061 ctatctggcg gcggcgaaag tgggcggcat tgttgccaac aacacctatc cggcggattt
    15121 catctaccag aacatgatga ttgagagcaa catcattcac gccgcgcatc agaacgacgt
    15181 gaacaaactg atgtttctcg gatcgtcctg catctacccg aaactggcaa aacagccgat
    15241 ggcagaaagc gagttattgc agggcacgct ggagccgacc aacgagcctt acgccattgc
    15301 caaaatcgcc gggatcaaac tgtgcgaatc ttacaaccgc cagtatggac gcgattaccg
    15361 ctctgtcatg ccgaccaacc tgtacgggcc gcatgacaac ttccacccga gtaactccca
    15421 tgtgatacca gcatcgctgc gtcgcttcca cgaggcgacg gcacagaatg cgccggacgt
    15481 ggtggtatgg ggcagcggta caccgatgcg tgaattcctg cacgtcgatg atatggcggc
    15541 ggcgagcatt catgtcatgg agctggcgca tgaagtctgg ctggagaata cccaaccgat
    15601 gctgtcgcac attaacgtcg gcacgggcgt tgactgcact atccgcgagc tggcgcaaac
    15661 catcgccaaa gtggtgggtt acaaaggtcg ggtggttttt gatgccagca aaccggatgg
    15721 tacgccgcgc aaactgctgg atgtgacgcg cctgcatcag cttggctggt atcacgaaat
    15781 ctcactggaa gcggggcttg ccagcactta ccagtggttc cttgagaatc aagaccgctt
    15841 tcggggggaa tgatgttttt acgtcaggaa gactttgcca ctgtagtgcg ctccacaccg
    15901 cttgtctctc tcgactttat tgtcgagaac agtcgcggcg agtttctgct tggcaaaaga
    15961 accaaccgcc cggcgcaggg ttactggttt gtgccgggag ggcgcgtgca gaaagacgaa
    16021 acgctggaag ccgcatttga gcggctgacg atggcggaac tggggctgcg tctgccgata
    16081 acagcaggcc agttttacgg tgtctggcag catttttatg acgataactt ctccggcacg
    16141 gatttcacca ctcactatgt ggtgctcggt tttcgcttca gagtagcgga agaagagctg
    16201 ttactgccgg atgagcagca tgacgattac cgctggctga cgccggacgc gctgctcgcc
    16261 agtaacgatg ttcacgctaa cagccgcgcc tattttctcg ctgagaagcg tgccggagta
    16321 cccggattat gaaaatactg gtctacggca ttaactactc gccggagtta accggcatcg
    16381 gcaaatacac cggcgagatg gtggaatggc tggcggcaca aggtcatgag gtgcgggtca
    16441 ttaccgcacc gccttactac ccgcaatggc aggtgggtga gaactattcc gcctggcgct
    16501 acaaacgaga agagggggcc gccacggtgt ggcgctgccc gctgtacgtg ccaaaacagc
    16561 cgagcaccct gaaacgcttg ttgcatctcg gcagttttgc cgtcagcagt ttctttccac
    16621 tgatggcgca acgtcgctgg aagccggatc gcattattgg cgtagtgcca acgctgtttt
    16681 gcacgccggg aatgcgcctg ctggcgaagc tctctggtgc gcgtaccgtg ctgcatattc
    16741 aggattacga agtggacgcc atgctggggc tgggccttgc cggaaaaggc aaaggcggca
    16801 aagtggcaca gctggcaacg gcgttcgaac gtagcggact gcataacgtc gataacgttt
    16861 ccacgatttc gcgttcgatg atgaataaag ccatcgaaaa aggcgtggcg gcggataacg
    16921 tcatcttctt ccccaactgg tcggaaattg cccgttttca gcatgttgca gacgccgatg
    16981 ttgatgccct tcgtaaccag cttggcctgc cggataacaa aaaaatcatt ctttactccg
    17041 gcaatattgg tgaaaagcag gggctggaaa acgttattga agctgccgat ctcctgcgcg
    17101 atgaaccgct gatttttgcc attgtcgggc agggcggcgg caaagcgcgg ctggaaaaaa
    17161 tggcgcagca gcgtggactg cgcaacatgc aatttttccc gctgcaatcg tatgacgctt
    17221 tacccgcact gctgaagatg ggcgattgcc atctggtggt gcaaaaacgc ggcgcggcag
    17281 atgccgtatt gccgtcgaaa ctgaccaata ttctggcggt aggcggtaac gcggtgatta
    17341 ccgcagaggc ccacacagaa ctggggcagc tttgcgaaac ctttccgggt attgcggttt
    17401 gcgtagaacc ggaatcggtc gaggcgctgg tggcggggat tcgtcaggcg ctcctgctgc
    17461 ccaaacataa cacggtggca cgtgaatatg ccgaacgcac gctcgataaa gagaacgtgt
    17521 tacgtcaatt tataaatgat attcggggat aattatggcg cagtcgaaac tctatccagt
    17581 tgtgatggca ggtggctccg gtagccgctt atggccgctt tcccgcgtac tttaccccaa
    17641 gcagttttta tgcctgaaag gcgatctcac catgctgcaa accaccatct gccgcctgaa
    17701 cggcgtggag tgcgaaagcc cggtggtgat ttgcaatgag cagcaccgct ttattgtcgc
    17761 ggaacagctg tgtcaactga acaaactcac cgagaacatt attctcgaac cggcagggcg
    17821 taacactgca cctgccattg cactggcggc gctggcggca aaacgtcata gcccggagag
    17881 cgacccgtta atgctggtat tggcggcgga tcatgtgatt gccgatgaag acgcgttccg
    17941 tgccgccgtg cgtaatgcca tgccttatgc cgaagcgggc aagctggtga cctttggcat
    18001 tgtgccggat ctaccagaaa ccggttatgg ctatattcgt cgcggtgaag tgtctgcggg
    18061 tgagcaggat acggtggcct ttgaagtggc gcagtttgtc gaaaaaccga atctggaaac
    18121 cgcccaggcc tatgtagcaa gcggcgaata ttactggaac agcggcatgt tcctgttccg
    18181 cgccggacgc tatctcgaag aactgaaaaa atatcgcccg gatatcctcg atgcctgcga
    18241 aaaagcgatg agcgccgtcg atccggatct cgattttatt cgtgtggatg aagaagcgtt
    18301 tctcgcctgc ccggaagagt cggtggatta cgcggtcatg gaacgtacgg cagatgctgt
    18361 tgtggtgccg atggatgcgg gctggagcga tgttggttcc tggtcttcat tatgggagat
    18421 cagcgcccac accgccgagg gcaacgtttg ccacggcgat gtgattaatc acaaaactga
    18481 aaacagctat gtgtatgccg aatcaggcct ggtcaccacc gtcggggtga aagatctggt
    18541 agtggtgcag accaaagatg cggtgctgat tgccgaccgt aacgcggtac aggatgtgaa
    18601 aaaagtggtc gagcagatca aagccgatgg tcgccatgag catcgggtgc atcgcgaagt
    18661 gtatcgtccg tggggcaaat atgactctat cgacgcgggc gaccgctacc aggtgaaacg
    18721 catcaccgtg aaaccgggcg agggtttgtc ggtacagatg caccatcacc gcgcggaaca
    18781 ctgggtagtg gtcgcgggaa cggcaaaagt caccattgat ggtgatatca aactgcttgg
    18841 tgaaaacgag tccatttata ttccgctggg ggcgacgcac tgcctggaaa acccggggaa
    18901 aattccgctc gatttaattg aagtgcgctc cggctcttat ctcgaagagg atgatgtggt
    18961 gcgcttcgcg gatcgctacg gacgggtgta aacgtcgcat caggcgctgt ttgtcggatg
    19021 cggcgtgaac gccttatccg acctacggtt cggttttgta ggcctgataa gacgcggcag
    19081 cgtcgcatca ggcaatgaat gcgaaaccgc ggtgtaaata acgacaaaaa taaaattggc
    19141 cgtttcggtc agggccaact attgcctgaa aaagggtaac gacatgaaaa aattaacctg
    19201 ctttaaagcc tatgatattc gcgggaaatt aggcgaagaa ctgaatgaag atatcgcctg
    19261 gcgcattggt cgcgcctatg gcgaatttct caaaccgaaa accattgtgt taggcggtga
    19321 tgtccgtctc accagcgaaa ccttaaaact ggcgctggca aaaggtttac aggatgcggg
    19381 cgtcgatgtg ctggatatcg gtatgtccgg caccgaagag atctatttcg ccacgttcca
    19441 tctcggcgtg gatggcggca ttgaagttac tgccagccat aatccgatgg attacaacgg
    19501 catgaagctg gtgcgcgagg gggctcgccc gatcagcggt gataccggac tgcgcgacgt
    19561 ccagcgtctg gctgaagcca acgactttcc tcccgtcgat gaaaccaaac gcggtcgcta
    19621 tcagcaaatc aacctgcgtg acgcttacgt tgatcacctg ttcggttata tcaatgtcaa
    19681 aaatctcacg ccgctcaagc tggtgatcaa ctccgggaat ggcgcagcgg gtccggtggt
    19741 ggacgccatt gaagcccgct ttaaagccct cggcgcgccc gtggaattaa tcaaagtaca
    19801 caacacaccg gacggcaatt tccccaacgg tattcctaac ccgctgctgc cggaatgtcg
    19861 cgacgacacc cgcaatgcgg tcatcaaaca cggcgcggat atgggcattg cctttgatgg
    19921 cgattttgac cgctgttttc tgtttgacga aaaagggcag tttatcgagg gctactacat
    19981 tgtcggcctg ctggcagaag cgttcctcga aaaaaatccc ggcgcgaaga tcatccatga
    20041 tccgcgtctc tcctggaaca ctattgatgt ggtgactgct gcaggcggca cgccggtaat
    20101 gtcgaaaacc ggacacgcct ttattaaaga acggatgcgc aaggaagacg ccatctacgg
    20161 tggcgaaatg agcgcccacc attacttccg tgatttcgct tactgcgaca gcggcatgat
    20221 cccgtggctg ctggtcgccg aactggtgtg cctgaaagga aaaacgctgg gcgaactggt
    20281 gcgcgaccgg atggcggcgt ttccggcaag cggtgagatc aacagcaaac tggcgcaacc
    20341 cgttgaggcg attaaccgcg tggaacagca ttttagccgt gaggcgctgg cggtggatcg
    20401 cagcgatggc atcagcatga cctttgccga ctggcgcttt aacctgcgca cctccaatac
    20461 cgaaccggtg gtgcgcctga atgtggaatc gcgcggtgat gtgccgctga tggaagcgcg
    20521 aacgcgaact ctgctgacgt tgctgaacga gtaatgtcgg atcttccctt accccactgc
    20581 gggtaagggg ctaataacag gaacaacgat gataaatcta aaaaagcgtg agcgagcgaa
    20641 aaccaatgca tcgttaatct ctatggtgca acgcttttca gatatcacca tcatgtttgc
    20701 cgggctatgg ctggtttgcg aagtcagcgg actgtcattc ctctacatgc acctgttggt
    20761 ggcgctgatt acgctggtgg tgttccagat gctgggcggc atcaccgatt tttatcgctc
    20821 atggcgcggt gttcgggcag cgacagaatt tgccctgttg ctacaaaact ggaccttaag
    20881 cgtgattttc agcgccggac tggtggcgtt caacaatgat ttcgacacgc aactgaaaat
    20941 ctggctggcg tggtatgggc tgaccagcat cggactggtg gtttgccgtt catgtattcg
    21001 cattggggcg ggctggctgc gtaatcatgg ctacaacaag cggatggtcg ccgtagcggg
    21061 ggatttagcc gccggacaaa tgctgatgga gagtttccgt aatcagccgt ggttagggtt
    21121 tgaagtggtg ggcgtttacc acgacccgaa accgggcggc gtttctaacg actgggcggg
    21181 taacctgcaa cagctggtcg aggatgcaaa agcgggcaag attcataacg tctatatcgc
    21241 gatgcaaatg tgcgacggcg cacgagtgaa aaaactggtc catcaactgg cggataccac
    21301 ctgttcggtg ctgctgatcc ccgacgtctt taccttcaac attctccatt cacgtctcga
    21361 agagatgaac ggcgttccgg tggtgccgct gtatgacacg ccgctttcgg gggttaaccg
    21421 cctgctaaaa cgtgcggaag acattgtgct ggcgacgctt attctgctgc tgatctcccc
    21481 ggtgctgtgc tgtattgcgc tggcggtgaa actcagttca ccagggccgg ttattttccg
    21541 ccagactcgc tatggtatgg atggcaagcc gatcaaagtg tggaagttcc gttccatgaa
    21601 agtgatggag aacgacaaag tggtgaccca ggcgacgcag aacgatccgc gcgtcactaa
    21661 agtggggaac tttctgcgcc gcacctcgct ggacgaattg ccgcagttta tcaatgtgct
    21721 gaccgggggg atgtcgattg tcggtccacg tccgcacgca gtagcgcata acgaacagta
    21781 tcgccagctc attgaaggct acatgctgcg ccataaagtg aaaccgggca ttaccggctg
    21841 ggcgcagatt aatggctggc gcggcgaaac cgacacgctg gagaaaatgg aaaaacgcgt
    21901 cgagttcgac cttgagtaca tccgcgaatg gagcgtctgg ttcgatatca aaatcgtttt
    21961 cctgacggta ttcaagggct tcgttaacaa agcggcatat tgatatgagc ttacgtgaaa
    22021 aaaccatcag cggcgcgaag tggtcggcta ttgccacggt gatcatcatc ggcctcgggc
    22081 tggtgcagat gaccgtgctg gcgcggatta tcgacaacca ccagttcggc ctgctgactg
    22141 tgtcgctggt gattatcgcg ctggcagata cgctttctga cttcggtatc gctaactcga
    22201 ttattcagcg aaaagaaatc agtcaccttg aactcaccac tctgtactgg ctgaacgtcg
    22261 ggctggggct tgtggtatgt gtggcggtgt ttttgttgag tgatgtcatc ggcgacgtgc
    22321 tgaataaccc ggacctggca ccgttgatta aaacattgtc gctggcgttt gtggtgatcc
    22381 cccacgggca acagttccgc gcgttgatgc agaaggagct ggagttcaac aaaatcggca
    22441 tgatcgaaac cagcgcggtg ctggcgggct tcacttttac ggtagttagc gcccatttct
    22501 ggccgctggc gatgaccgcg atcctcggtt atctggtcaa tagtgcggtg agaacactgc
    22561 tgtttggcta ctttggacgc aaaatttatt gccccggtct gcacttctcg ctggcgtctg
    22621 tggcaccgaa cttacgtttt ggtgcctggc tgacggcgga cagcatcatc cactatctca
    22681 acaccaacct ttcaacgctg gtgctggcgc gtattcttgg cgcgggcgtg gcagggggat
    22741 acaacctggc gtacaacgtg gccgtcgtac cgccgatgaa gctgaaccca atcatcaccc
    22801 gcgtgttgtt tccggcgttt gccaaaattc aggacgacac cgaaaagctg cgtgttaact
    22861 tctacaagct gctgtcgata gtggggatta tcaactttcc ggcgctgctg gggctgatgg
    22921 tggtgtcgaa taacttcgta ccgctggtat tcggcgaaaa gtggaacagc attattccgg
    22981 tgctgcaatt gctgtgtatt gtgggtctgc tgcgctccgt gggtaacccg attggttcgc
    23041 tgctgatggc gaaagcgcgg gtcgatatca gctttaaatt caacgtattc aaaacatttc
    23101 tgtttattcc ggcgattgtt attggtgggc agatggcggg cgcaatcggc gtcacgctcg
    23161 gtttcctgct ggtgcagatt atcaacacca ttctgagtta cttcgtgatg attaaaccgg
    23221 ttcttggttc cagttatcgc cagtacatcc tgagtttgtg gctgccgttt tatctctcgt
    23281 tgccgacgct ggtggtcagt tatgcgctgg gcattgtgct gaaagggcaa ctggcgctgg
    23341 ggatgctgct ggcggtgcaa atagccgcgg gcgtgctggc gtttgtggtg atgattgtgc
    23401 tgtcacgcca tccgctggtg gtggaagtga agcgtcagtt ttgtcgcagc gaaaaaatga
    23461 aaatgctttt acgggcgggg tgaattgctg ttttcggttc cgacaacggt tgccggatgc
    23521 ggcgtaaaac gccttatccg tcctacggtt ctgtgctcgg gacctgtagg cctgataaga
    23581 cgcgccagcg tcgcatcagg caaaaaacag caacgaacac aacaaacaca gcctcacctg
    23641 gcatcaaatt tatgaggaaa aaatgaaatt acttattctg ggcaaccaca cttgcggcaa
    23701 tcgtggcgac agcgccatct tgcgcggctt acttgatgcc atcaacatcc tcaatccaca
    23761 taccgaagtg gacgtgatga gccgctatcc ggtcagttct tcctggctgc tcaaccgccc
    23821 ggtaatgggc gatccgctgt tcctgcagat gacacaacac aatagcgcgg cgggcgttgt
    23881 cgggcgcgtt aaaaaagtcc tccgtcgccg ctaccagcat caggtattgc tctcacgcgt
    23941 caccgacact ggcaagctgc gcaatatcgc catcgcccag ggattcaccg acttcgtgcg
    24001 cctgctgtca ggttacgacg ccattattca ggtcggcgga tcgttttttg tcgatctcta
    24061 cggcgtgccg cagtttgaac atgcgctttg cacatttatg gcgaaaaagc cgctgtttat
    24121 gattggtcac agcgtcggcc ccttccagga tgagcaattt aaccaactgg cgaactatgt
    24181 ctttggtcac tgcgacgcgc tgatcctgcg cgaatcggtc agccttgatt tgatgaaacg
    24241 cagcaatatc accaccgcaa aagtggaaca tggcgtcgat accgcgtggc tggtcgatca
    24301 ccacaccgaa gacttcaccg ccagctatgc cgttcaacac tggctggacg ttgccgcaca
    24361 acagaaaaca gtggcaatta ccctgcgcga actggcaccg ttcgacaaac gtctcggcac
    24421 cactcaacaa gcgtatgaaa aagcctttgc cggggtggtc aatcgcattc tcgatgaagg
    24481 ctatcaggtg attgcgctct ccacctgtac gggtatcgac agctataaca aagacgaccg
    24541 catggtggcg ctcaatctgc gccagcacat cagcgatcct gcccgttacc acgtagtgat
    24601 ggatgaactc aacgatctgg aaatgggcaa aattctcgga gcctgtgaac tcaccgtcgg
    24661 tacgcgcctg cactccgcca ttatctcaat gaactttgct actccggcga ttgccatcaa
    24721 ctatgaacat aaatccgccg ggattatgca gcagctggga ctgccagaga tggcgattga
    24781 tatccgtcat ttactggacg gcagcctgca agcgatggtt gcggatacct taggccagct
    24841 tccggtgctg aacgctcgac ttaacgaagc cgtcagtcgc gaacgtcaga cgggaatgca
    24901 gatggtgcag tctgtgcttg agcgcatcgg ggaggtgaaa tgaaggtcgg cttcttctta
    24961 ttgaaattcc cgctgtcgtc ggaaaccttc gtcctcaatc aaattaccgc gtttattgat
    25021 atgggctttg aagtggagat tgtcgctttg caaaaaggcg atacagagaa cacccacgcg
    25081 gcatggacga aatacaacct tgtcgccaga acccgctggt tacaggacga acctgcgggc
    25141 aaagtagcga aactgcgcca ccgcgccagc cagaccttac gtggcattca tcgtaaaaat
    25201 acctggcatg cgctcaacct caaacgctat ggtgccgagt cgcggaacct gattttgtct
    25261 gccatttgtg gtcaggtcgc aacaccgttt cgcgccgatg tgttcatcgc tcattttggc
    25321 ccggcggggg taaccgcagc aaaactacgc gaactgggta tcattcgcgg caaaattgcc
    25381 accatcttcc acggtattga tatctccagt cgggaagtgc tcaaccacta cactcccgaa
    25441 tatcaacaac tgtttcgccg tggcgacctg atgttaccga taagcgatct gtgggccgga
    25501 aggctgcaaa aaatgggctg ccccagagaa aaaatcgccg tatcgcgtat gggcgtggac
    25561 atgacgcgct ttagcccgcg tccggtgaaa gcgcccgcaa cgccgctgga aatcatatct
    25621 gtcgcacgct taaccgagaa aaaaggcctg catgtggcga ttgaagcctg ccgtcagttg
    25681 aaagagcagg gcgtggcatt tcgctatcgc atcctcggca ttggcctgtg ggaacgacgc
    25741 ctgcgcaccc tcatcgaaca atatcaactg gaagatgtgg tagagatgcc gggctttaaa
    25801 ccgagccatg aagtgaagga gatgctcgac gacgcggatg tcttcctgtt gccatcggta
    25861 acgggcgcgg atggcgatat ggaaggcatt ccggtggcgc taatggaagc gatggcggtc
    25921 ggtattccgg tggtttctac tctgcatagc ggaattcctg aactggtgga ggccgataaa
    25981 tccggctggc tggtgcctga gaacgatgct cgcgcactgg cacaacgtct ggcggcgttt
    26041 agccaactgg ataccgacga actggctccg gtcgtcaaac gtgcgcgcga aaaagtcgaa
    26101 cacgatttta accagcaggt gattaatcga gaactcgcca gcttgctgca ggctttatag
    26161 aggagaacgt atgccattta aaacactctc ccgacgcacc ttcctgacgg caagctcggc
    26221 gcttgccttc ctccataccc ctttcgctcg cgcacttccc gcccgacaaa gcgttaacat
    26281 taacgactac aacccacatg actggatcgc ctcatttaaa caagccttca gcgaagggca
    26341 aacggtcgtc gtgcctgccg gattcgtttg tgacaatatc aacaccggca tcttcatccc
    26401 tcccggcaaa acgttacaca tccttggaag cctgcgcggc aacggcagag ggcgatttgt
    26461 cttacaggac ggcagccagg tgacaggggg agagggcggc ggtatgcata acattaccct
    26521 ggatgtgcgt ggttctgact gcaccatcaa agggctggcg atgagcggct ttggcccggt
    26581 aatgcagatt tatattggcg gcaaaaacaa acgggtcatg cgcaacctga ccatcgataa
    26641 cctcactgtc agccacgcta attacgccat cttacgccag ggatttcata accagattat
    26701 cggtgccaac attaccaact gtaagttcag cgacttacag ggcgacgcca ttgaatggaa
    26761 cgtggcaatt aacgacagtg atattttgat atctgaccat gtcatcgagc gtatcaactg
    26821 taccaacggc aaaatcaact ggggcatcgg cataggcctt gcgggaagca cttatgataa
    26881 caactacccg gaagaccagg cagtgaaaaa ctttgtcgtg gcgaatatca cgggatcgga
    26941 ttgtcggcag ttgatccatg ttgaaaatgg taaacatttt gttattcgta atatcaatgc
    27001 ccgcaatatc acgccggatt tcagtaagaa agcgggcatt gataacgcga cagtcgctat
    27061 ttacggttgt gacaatttcg tgattgataa tattgaaatg attaatagcg ccgggatgtt
    27121 aatcggctat ggggtaatta aaggcaaata tctctcgata ccgcaaaatt tccgagtgaa
    27181 taatattcaa ctggataata cccatcttgc ttataaattg cgcggcatcc aaatctccgc
    27241 cgggaatgcc gtctcctttg tgtcgctaac taacattgag atgaagcgtg cctcgctgga
    27301 gttacacaac aagccgcaac atctttttat gcgaaatatc aatgtgatgc aggaatcctc
    27361 agttggacca gcattgagca tgaacttcga catgcgcaaa gacgttcgcg gcgtctttat
    27421 ggcgaaaaaa gaaacactgc tgtctcttgc aaatgttcat gcggtgaatg aaagagggca
    27481 aagctccgtc gatatcgaca ggattaatca ccatattgtt aatgtggaaa agattaactt
    27541 tagattgccg gaacggaggg agtagatttg cgaccattcc tggaaaaatg gagccatact
    27601 taggaacaat gctactgcaa tccacaacga agcggcgtaa catcacaagt aattcagtaa
    27661 tcaattcagg gtaattgatg ctggcgaaaa aaatcgaaca agctataatt cagcaaccat
    27721 tttacaggtg gatgaaataa tgacgaattt aaaagcagtt attcctgtag cgggtctcgg
    27781 gatgcatatg ttgcctgcca ctaaggcgat acccaaagag atgctaccaa tcgtcgacaa
    27841 gccaatgatt cagtacattg ttgacgagat tgtggctgca gggatcaaag aaatcctcct
    27901 ggtaactcac gcgtccaaga acgcggtcga aaaccacttc gacacctctt atgagttaga
    27961 atcactcctt gagcagcgcg tgaagcgtca actgctggcg gaagtacagt ccatctgtcc
    28021 gccgggcgtg accattatga acgtgcgtca gggcgaacct ttaggtttag gccactccat
    28081 tttgtgtgcg cgacctgcca ttggtgacaa cccatttgtc gtggtactgc cagacgttgt
    28141 gatcgacgat gccagcgccg acccgctacg ttacaacctt gctgccatga ttgcacgttt
    28201 caacgaaacg ggccgcagcc aggtgctggc aaaacgtatg ccgggtgacc tctctgaata
    28261 ctccgtcatc cagactaaag agccgctgga ccgtgagggt aaagtcagcc gcattgttga
    28321 atttatcgaa aaaccggatc agccgcagac gctggactca gacatcatgg ccgtaggtcg
    28381 ctatgtgctt tctgccgata tttggccgga actggaacgt actcagcctg gtgcatgggg
    28441 acgtattcag ctgactgatg ctattgccga gctggcgaaa aaacaatccg ttgatgcaat
    28501 gctgatgacc ggcgacagtt acgactgcgg caaaaaaatg ggctatatgc aggcgtttgt
    28561 gaagtatggc ctacgcaacc tgaaagaagg ggcgaagttc cgtaaaggta ttgagaagct
    28621 gttaagcgaa taatgaaaat ctgaccggat gtaacggttg ataagaaaat tataacggca
    28681 gtgaagattc gaggcataag ttttgctcat tgaattttcc tgccgttgcc gtttttataa
    28741 agcctataaa aacaagaagt tacatagctc atattattca aatttttcca ggattttcct
    28801 tgtttctacg gctgtttggt aagacaatta acgtttgaat ttttcggatt tggcgcgagt
    28861 ggctaacgct cgccacatcg ttgagatgca tgcagtgcac tggtagctgt taagccaggg
    28921 gcggtagcgt gtattaatac ctctattaat caaatctgga gcagtctatt tcacagcatg
    28981 ctctatagct atatggaata aaaaagtgaa gatacttgtt actggtggcg cagggtttat
    29041 tggttctgct gttgttcgtc acataataaa taatacgcaa gatagtgttg ttaatgtcga
    29101 taaattaaca tacgccggga acctggaatc actcgctgat gtttctgatt ctgaacgtta
    29161 tgcttttgag catgcaggta tctgcgatgc tgtagccatg tcgcgtattt tcgcacagca
    29221 ccagccagac gcggtgatgc acctggcggc agagagccac gttgaccgtt caataacggg
    29281 ccctgcggca tttattgaaa ccaatattgt ggggacttat gttcttttag aagcggcgcg
    29341 caattactgg tctgctctga atgatgaaaa gaaaaaaagt ttccgctttc atcatatttc
    29401 tactgatgaa gtttatggtg acttacccca tccggatgaa gcaaataata acgaagcgtt
    29461 accgctattt acggaaacga cagcttacgc gccaagtagc ccgtattctg cgtctaaagc
    29521 ttccagcgat catttggtac gtgcctggaa gcggacttat ggtttaccaa ccattgtgac
    29581 taattgctcg aacaattatg gtccttatca tttcccggaa aaacttattc cattggttat
    29641 tctgaatgct ctggaaggta aggcattacc tatttatggt aaaggggatc aaattcgcga
    29701 ctggttgtat gtagaggatc atgctcgtgc gttatatacc gtcgtaaccg aaggtaaagc
    29761 gggtgaaact tataacattg gtggacataa cgaaaagaaa aacatcgacg tagtgctcac
    29821 tatttgtgat ttgttggatg agattgtacc gaaagagaaa tcttatcgtg agcaaattac
    29881 ttatgttgcc gatcgcccgg ggcatgaccg ccgttacgcc attgatgccg ataaaattag
    29941 ccgcgaattg ggctggaaac cgcaagaaac gtttgagagc gggattcgta aaacggtgga
    30001 atggtatctg gctaatacaa attgggttga gaatgtgaaa agcggtgctt atcagtcgtg
    30061 gattgaacaa aactatgagg gccgtcagta atgaatatcc tgcttttcgg caaaacaggg
    30121 caggtgggtt gggaactgca gcgtgctctg gctcctctgg gtaacttgat tgcgctcgat
    30181 gttcactcca ctgattattg tggagatttc agcaaccccg aaggtgtggc tgaaaccgtc
    30241 aaaaaaattc gccccgatgt tattgttaat gctgctgctc acactgcagt agacaaagcg
    30301 gaatcagaac cgaatttcgc gcaattactt aacgcgacat gcgtcgaagc gattgcaaaa
    30361 gcagctaatg aagtcggagc ctgggttata cactactcca ctgattatgt tttcccaggc
    30421 aacggtgaca cgccatggct ggaaacggat gcaacagcac cgctaaatgt ctacggtgaa
    30481 accaagctag ctggggaaaa agcgttacaa gaacattgcg caaagcatct tattttccgt
    30541 accagctggg tatacgctgg taaaggaaat aactttgcca aaacgatgtt gcgcctggca
    30601 aaagaacgcg aagagttggc tgtaataaac gatcaattcg gcgcaccaac gggggctgaa
    30661 ttactggctg attgcaccgc tcatgccatt cgcgtggccg caaataaacc agaagttgct
    30721 ggtttgtacc atctggttgc tggtggtaca acaacctggc acgattatgc cgctctggta
    30781 tttgaagaag cgcgcagagc ggggatcaac cttgctctta acaaacttaa tgccgtgccg
    30841 acaacggcct atcccacacc agcacgtcgt ccccataatt ctcgcctcaa taccgaaaaa
    30901 tttcagcaga actttgcgct tgtcttgcct gactggcagg tgggcgtgaa acgtatgctc
    30961 aacgaattat ttacgaccac ggcaatttaa caaattattg catctcgctc atgatgccag
    31021 agcgagatga attaaaagga atggtgaaat gaaaacgcgt aaaggtatta ttctggctgg
    31081 tggttccggc actcgtcttt atcctgtgac tatggctgtg agcaaacaac tgctaccgat
    31141 ttatgataaa ccgatgattt attatccgct ttcaacgctt atgttggcgg gtattcgaga
    31201 tattctgatt atcagtacgc cgcaggatac accacgtttt caacaattgc ttggtgatgg
    31261 aagccagtgg gggcttaatc tgcaatataa agtacaaccg agtccggatg gtctggcgca
    31321 agcatttatc attggtgaag aatttatagg tggtgatgat tgtgcgctgg tacttggtga
    31381 taatatcttc tatggtcacg acctgccgaa attaatggat accgctgtta acagagagag
    31441 tggcgcaacg gtttttgcct atcacgtaaa tgatccagag cgctacggtg tggtggaatt
    31501 tgatgataac ggtacggcaa ttagtctgga agaaaaaccg ctggaaccaa aaagtaacta
    31561 tgcggttact gggctttatt tctatgacaa tgacgttgta gaaatggcga aaaaccttaa
    31621 accatctgca cgtggcgaac tggaaattac cgatattaac cgtatttata tggaccaagg
    31681 acgtttgtct gtcgctatga tggggcgtgg ttatgcatgg ctggacacgg ggacacatca
    31741 aagtcttatt gaagcaagca acttcattgc aacaattgaa gagcgtcagg ggctgaaagt
    31801 ttcctgcccg gaagaaattg cttaccgtaa aggctttatt gatgctgagc aagtgaaggt
    31861 attagccgag ccgctgaaga aaaatgctta tggtcagtat ctgctgaaaa tgattaaagg
    31921 ttattaataa aatgaacgta attaaaactg aaattcctga tgtgctaatt tttgaaccaa
    31981 aagtatttgg tgatgagcgg ggctttttta tggaaagctt caatcagaaa gttttcgaag
    32041 aggctgtagg acgaaaggtt gaatttgttc aggataacca ttctaaatca actaagggtg
    32101 tgttacgcgg actgcactat cagctggaac cttatgctca aggaaaactg gtacgctgtg
    32161 ttgtcggtga agtttttgac gtagctgttg atattcggaa atcatcacca acatttggca
    32221 agtgggttgg tgtaaatttg tcggcggaga ataagcgtca attatggata ccggaaggat
    32281 tcgctcatgg gttttgcgtt cttagtgatg aagcagaatt tgtctacaaa acaaataatt
    32341 tctattcaaa aatgcaagag cgtgggattt tatggagcga caaaagtata aatatagaat
    32401 ggcaggttca aaatccattg ctttctgata aagatattaa tggtcaaaaa tttgtagatg
    32461 ctgattattt tatatgataa agaaatgtaa taatgagcat aataaaaaat agtgtctgga
    32521 acctttttgg ctatgcaata ccaactttaa ttgctatccc ctcgctagga tttctcgctc
    32581 gaggtttggg gcctgaaggt ttcggtgttt atacaattgc aattgcactt gttgggtatg
    32641 ctggaatttt tgatgtaggc ctgactcgct ctgttattag agaaattgca attcatcgtg
    32701 ataatcatca tgaaagaacc aaggtaattt caacaagtac atcttttttg gtgctatttt
    32761 catgctttgg tgctttttta ttattgattt tctctgatgg aattgttaat tatctaaaaa
    32821 tttctggtgt tgaacatagt gatatacaac tagcatttaa actgttggct atttgcattc
    32881 cattatttat tctaaatcaa ttatggtcag ccattcttga gggggatgaa aaatttggca
    32941 ttgtaaatat tcaaaaatct atatcaagct cttgcatcgc gggaattccg gccatatttg
    33001 ttttttatag tgctacattg tcggcagcgg ttgctggttt aatatttgca agagttattt
    33061 cgattttagt ctctgcctat tatgtcagga atgatattaa aatttcgggg gttcattttt
    33121 gttataaaac ctttaaacga ctctttttct ttggcggttg gatgacagta agtaatatca
    33181 taagtccggt catggtgtat tttgatcgat ttatagtgtc aaatattatg ggggcagata
    33241 aagttgcatt ttattctgca ccagcggagg ttattctaaa attaggaata atacctgcag
    33301 caatcgggag ggcagtgttt ccaaggttaa gtaacatcaa agactttaaa gaatttaaac
    33361 gtaatgtaaa taaatcattg cttttaatgt ttctaatctg tttgccggtg ataatcatag
    33421 gcttgttata ttcagggctt gtattgaaaa tatggtttgg tgagaattac caaattaatt
    33481 cctttaatat attaaatgtg ttattgatcg gttttttctt caatgcgctg gcaatgatac
    33541 cattctctgc aatccaggca ttaggaaaat ctaaaattac tgctttgatt cattgtgctg
    33601 aattggttcc ttatttagcc cttttgtatt ttatggtcga aaaatatggg ttactgggtg
    33661 ccgcaatatc ctggagcata cgtgtaattt tagatgctct gttattacaa tggctttata
    33721 ctagaatgtg ttctgtatat gaaaactaaa attaaccaat tactgagtca cttggataat
    33781 gaatagtaat atttacgctg tcattgtgac atataatccc gaacttaaaa atctgaatgc
    33841 actgatcact gaactaaagg agcaaaattg ttacgttgta gtagtcgata atagtactaa
    33901 tttcaccttg aaagataaat tagcagacat tgaaaaagtt catttaattt gtcttggtag
    33961 gaatgaaggt atagcaaaag cacaaaatat tgggattcga tattctcttg aaaaaggggc
    34021 agagaagata attttttttg atcaagatag ccgtattcgc aatgagttta tcaaaaaact
    34081 ttcctgctat atggataatg aaaatgcaaa aatagctggc ccagttttta ttgatagaga
    34141 taagtcacat tattatccta tttgtaatat caaaaaaaat ggtcttcgag agaaaattca
    34201 tgtcactgaa ggacagacac cgtttaaaag ttcagtaaca atctcatccg gaaccatggt
    34261 ttcaaaagaa gtttttgaga ttgttggaat gatggatgag gaacttttta ttgattatgt
    34321 cgatacagaa tggtgtctta gatgcttaaa ctatggcata ttagttcata tcattcctga
    34381 tatagaaatg gttcatgcta ttggggataa gtcagtaaaa atctgtggga ttaacatacc
    34441 aattcactcg ccagtacgtc gttattatcg agtaagaaat gcatttcttt tgcttagaaa
    34501 aaatcatgtg cctcttttac tctctattag ggaagttgtt ttttctttaa ttcatacgac
    34561 tttaattatc gcaactcaaa aaaataaaat tgaatatatg aaaaaacata ttttggctac
    34621 gctggatgga ataagaggaa ttactgggga cggtcgttat aatgcatagt tctgatcaaa
    34681 aaagagtagc tgtacttatg gctacgtata atggcgaatg ctggattgag gaacaattaa
    34741 aaagtattat tgaacaaaaa gatgtagaca tctcaatttt tatatcagat gatttatcaa
    34801 ctgataatac attaaatatt tgtgaagaat ttcaattatc atatccctca attataaata
    34861 tacttccaag cgtcaataag tttggggggg caggtaaaaa tttttataga ttaattaaag
    34921 atgtcgatct tgaaaactat gattatatct gttttagtga tcaggacgat atttggtata
    34981 aagataaaat aaaaaatgca attgactgct tggtatttaa taatgcgaat tgttattcca
    35041 gcaatgtaat tgcatattat ccatcagggc aaaaaaattt agttgataag gctcagtcgc
    35101 aaactcaatt cgattatttt tttgaagcag caggtccggg gtgtacatat gttataaaaa
    35161 aagaaacgct tattgaattt aagaagttca tcataaataa taaaaatgct gcacaagata
    35221 tctgtctgca tgattggttt ttatattcat ttgcgcggac gagaaattat tcgtggtata
    35281 ttgatagaaa accaacaatg ctgtacagac agcatgaaaa caaccaagtg ggagcaaaca
    35341 ttagttttaa agcaaaatac aaacggcttg gtttggtgag aaacaagtgg tatcgaaaag
    35401 aagtaactaa aatagctaat gccttagctg atgatagctt tgtgaataat caattgggta
    35461 aaggttatat aggcaatttg atcttggctt taagtttttg gaagttaagg cggaaaaagg
    35521 ctgataaaat ttatatttta ttaatgttga ttttaaacat attttaaaaa acatcacctt
    35581 acgaatatga ataatataaa taaaattttt ataacatttt tatgtattga actgattatt
    35641 ggtggtggtg gaagattact ggagccattg ggaatattcc ctttgcgata tttattattt
    35701 gtatttagtt ttatactttt aatttttaat ttagttacat tcaatttttc aatcacccaa
    35761 aaatgtgtca gtctttttat atggttgctt ttatttcctt tttatggctt ctttgtcggc
    35821 ttattagctg gtaataaaat aaatgatata ctgtttgatg tgcaaccata cctctttatg
    35881 ctgtcactta tatatctatt tacactaaga tatactttaa aagtattttt atgtgagatt
    35941 tttattaaaa tagttaatgc atttgcatta tatggatcac tgttatatat ttcatacata
    36001 attttgttga atttcggttt gttaaatttt aatttaattt atgaacactt atcattgact
    36061 agcgagttct tttttcgtcc cgatggggct tttttttcca aatccttcta cttttttggt
    36121 gtcggtgcga ttatcagttt tgtcgacaaa aaatatttaa aatgtctcat aatagtgctt
    36181 gcgatattat tgacagaatc aagaggtgta ttacttttta caacattatc actgttatta
    36241 gccagtttta aattacataa gctatattta aatactatta taataatatt gggcagcgtt
    36301 ctatttataa ttatgcttta catggtcgga tcacgcagtg aagattctga ctctgttaga
    36361 tttaatgatt tatattttta ttataaaaat gttgatttag cgacgttctt gtttggaaga
    36421 ggatttggtt catttatatt agatcgatta aggattgaaa tagtacctct tgagatactt
    36481 cagaaaacag gcgttattgg tgtatttata tcattagttc ctatgttgct tatctttttg
    36541 aaaggctatt ttttaaacag tgcaaaaaca tcattaatga tgtcgttaat actttttttc
    36601 agtattaccg tttctataac taatccattt ctttttacac ccatgggaat ttttattata
    36661 ggcgttgtag ttttatgggt attttctata gaaaatatcc aaattagtaa taacctcact
    36721 tctggagcaa aataaaggat gttaaaaata gggaagttat tgaccagttc tttttttagt
    36781 tattttttaa ttggaattgt caatactgct ttacattggg gggtatttta tgcgtgttat
    36841 aataatttag cttttggtca aggccgaagt aacattgttg gttttatatg tgcagcaacc
    36901 ttttcttttt ttgcaaatgc aagatgctca tttaaagtat ctgcaaccaa ggctagatat
    36961 tttatattta tattttttat gggggcgatg agttatttat tcggcgtttt atttgatctc
    37021 ttagctttat ctccaatatt tacattgttc accttttctc tttttagttt ggttttagga
    37081 tattgcgcat caaaatattt catttttcgt tagtaaacga tttgttaaaa acacaattac
    37141 ttgcacatta gctaaatagt ttatatgagg ggcttatgta tcactctttt aatatcgctg
    37201 taattattcc ttgttataat gagcagaagg caatagcaaa agtcataaat gatttcaaaa
    37261 cgaatattcc aactgcttct atatatgtat ttgataataa ttcgactgac agtacagcac
    37321 aagtggcaga ggatgctgga gcaacagttc actctgttcc tctgaaaggc aaggggaatg
    37381 tcgtaagacg tatgttctca gatgtagatg cggatattta tctaatggtt gatggagatg
    37441 acacttatga tgcaagttct gctccagaaa tgattaacca tctcataaaa aatcaattgg
    37501 atatggttgt aggatgtcgt caagagaatg gagatcaaaa tacatacaga aaagggcatc
    37561 gctgaggaaa taaactgttg actggtactg ttcagactat ctttaaaggg aattttactg
    37621 atatgttgtc ggggtatcgt gtattttcca gacgctatgt taaatctttt ccatgtctat
    37681 caaggggatt tgaaatcgaa actgagctta caattcatgc tttagaatta cgtatgaagt
    37741 atggtgaggt taatacaaaa tatggcgagc gatctgaagg gagtgtgagc aaactatcaa
    37801 cttggagtga tgggttcaag attctgaaaa caataattaa actttactcg cttgaacggc
    37861 cactatactt ttttagtatt atcggcgttt tacttgctgc tttgtctata attttaggat
    37921 tgcccatcat tgttgattat attgatacag gtttggtgag aagatttccg actgcctttt
    37981 taactgcctc catcatgtta tcgtcaataa tggcatttgt atgtggcatt attttgcatt
    38041 caaatacaac aactagacgt gaaatgaagg cattatttta tctttctgaa aaaaaactat
    38101 aaactgataa tgtaaataga gtattttact atgattgaat tgccgttgta tttgtatgtt
    38161 gtgtattgtt ttgcttttgg aatgacattt tttgtcattg gtaatattgt attgtcatct
    38221 ttttttaagg agggggtggc aggacttaac aaattttctt tattttttta ttgcctatgc
    38281 atagggatgt ctttaagtat tttgataatt tactttctgg gcatggtcgg gcaatttaat
    38341 aagattgcta tctttttaat ttttacggtg tgttgggttt taagcattat taaacgtcag
    38401 caatttagat ggctggcaat aaataatatt gagttcagta cgctgtttgt tattttattt
    38461 cttgttttaa tttttgcggt tacattgtta tcgtccctgc gagctcccgg tgattgggat
    38521 gatactatgt atcacttacc tttggctcgt tcattagttg agcaccatgc aattgttgtt
    38581 gaacaatatt tgcgttttcc tctgttcccg caaaatgcag atcttttaat ggctttaggt
    38641 ttacagttag gagatgtaag gttagctcag tttttagcaa atatctgttt ttttgtaata
    38701 gcctgtggac ttgttggctg ttcatgggaa attacgaaaa cttattatct tggcattatt
    38761 gctacaatcc tactatttac tatcaatcca cttaaagatc atcttggtta tgcatatatt
    38821 gatttaacgc tatctttgtt ttgctgcagc cagtattctt atatatattc gctcagaaaa
    38881 caataaaaca caaaatgaat taattttttt atgtggtttt tttattggaa ttgcttgtgg
    38941 cgtaaaacta tttggtgcca tatatggagc catcatattt gttatattga tagcgaaaaa
    39001 gagaaacata cagacatttc ttatttttag cttggcaaca attcttggtg gcatttattg
    39061 gtatgtccgt agtttttgga tctctggtga tccttttagt cctgctggag gaaatatatt
    39121 tggtcactac ctatggaatg aaattgatct acaagttcaa acagcagaac aagctcgaca
    39181 tggtataagt cctgccacat tagatttgca acaagcaatt aataaagttg gcagtgatgt
    39241 tgttttttat gctatactgt ctgtctttac acttaggaat aataaaatac tttgggtatt
    39301 tttctcgatt gttttaacat atgtcttatt ttggttttct gttacgcaag gtgacaggta
    39361 tttatcaccc atttacccat tgtcagtatt gctggttgcg atctcaattg caagtttaat
    39421 aaaagatatc aacattacaa attacaagtt gggacgatat tttatccttg tgtgtattat
    39481 tttttttcgg ggcgatcgtt gtattttgaa ttcaaatatt tttattctga atacttgaat
    39541 tttaattcac gcttagaaag tcgctatagc tatgaactga tgaagaaagc aagtgaatat
    39601 agtgagttat atggagataa tcttatccaa ttaggcttgg aggatggtat ttacttctat
    39661 aaagggatgg caattggtga cgtctttgga ttagcaagat atagtgattg gactattagt
    39721 aatcccgagt gtgaggtaat cccacaggat gatttaatag agaagatgaa atcttttaat
    39781 agttcgttta tttttatatc taaacgaagc tatgctaatt ttaatccaga aaagtatcca
    39841 aaattcaaag ttcttatgga tacacccaat ggcatactta tcgctataaa gtaagaagca
    39901 tgctgtaacc atatgtattc cttgattaga agatgaaaat cacgacggtt aataatataa
    39961 cggttaatag acggttaata atatagaaga caggagtaaa caatgtcaaa gcaacagatc
    40021 ggcgtagtcg gtatggcagt gatggggcgc aaccttgcgc tcaacatcga aagtcgtggt
    40081 tataccgtct ctattttcaa ccgctcccgc gaaaagacgg aagaagtgat tgctgaaaat
    40141 ccaggcaaga aactggctcc ttactatacg gtgaaagagt ttgttgaatc tctggaaacg
    40201 cctcgtcgca tcctgttaat ggtgaaagca ggtgcaggca cggatgctgc gattgattcc
    40261 ctgaaaccat acctcgataa aggtgacatc atcattgatg gtggtaatac cttcttccag
    40321 gacaccattc gtcgtaatcg tgagctttca gcagagggct ttaacttcat cggtaccggt
    40381 gtttccggcg gtgaagaggg cgcgctgaaa ggtccttcta ttatgcctgg cgggcagaaa
    40441 gaggcttatg agctggttgc gcctatcctg actaaaatcg ctgccgtggc agaagacggc
    40501 gagccatgcg ttacttatat tggtgccgac ggcgcaggcc attatgtgaa gatggttcac
    40561 aatggtattg aatacggaga tatgcagctc attgctgaag cgtattctct gctcaaaggt
    40621 ggcctgaatc tatctaatga agaactggcc cagactttca ccgagtggaa taacggtgaa
    40681 cttagcagct acctgatcga catcactaaa gatatcttca ccaaaaaaga tgaagacggt
    40741 aactacctgg ttgatgtgat tctggatgaa gcagcaaaca aaggcaccgg taaatggacc
    40801 agccagagcg cgctggatct cggcgaaccg ctgtcgctga ttaccgagtc tgtgtttgca
    40861 cgttatatct cttctctgaa agatcagcgc gttgccgcat ctaaagttct ctctggtccg
    40921 caagctcagt cagcaggcga caaggctgag ttcatcgaaa aagttcgcag tgcgctgtat
    40981 ctgggcaaaa tcgtttctta cgctcagggc ttctctcagc tgcgtgctgc gtctgaagaa
    41041 tacaactggg atctgaacta cggcgaaatc gcgaagattt tccgtgctgg ctgcatcatc
    41101 cgtgcgcagt tcctgcagaa aatcaccgat gcttatgccg aaaatccgca gatcgctaac
    41161 ctgctgctgg ccccgtactt caagcaaatt gccgatgact accagcaggc gctgcgcgat
    41221 gtcgtcgctt atgcagtaca gaacggtatc ccggttccga ccttcgccgc tgcggttgcc
    41281 tattacgata gctaccgtgc cgcggttctg cctgcgaacc tgatccaggc acagcgtgac
    41341 tatttcgggg cgcatactta taagcgcatt gataaagaag gtgtgttcca tactgaatgg
    41401 ctggattaat ctgatttaac caacaataaa attgaggccc ggcgtatatt gcaccgggct
    41461 tttttttgcc aaatatctta tagactaaat ttcactgctt aatattaact taataaacat
    41521 cagctattct tataaagaaa atctgaattg tttttcgttg cgttgaccat ctgacaacgt
    41581 agcgttaaaa cttttagcat cttatcacga tgctaaaaac atcatgattc acagttaagt
    41641 taattctgag agcatgaaat gaaaatcacc atttccggta ctggctatgt aggcttgtca
    41701 aacgggcttc taatcgcaca aaatcatgag gttgtggcat tagatatttt accgtcacgc
    41761 gttgctatgc tgaatgatcg gatatctcct attgttgata aggaaattca gcagtttttg
    41821 caatcagata aaatacactt taatgccaca ttagataaaa atgaagccta ccgggatgct
    41881 gattatgtca tcatcgccac tccaaccgac tatgatccta aaactaatta tttcaataca
    41941 tccagtgtag aatcagtaat taaagacgta gttgagataa atccttatgc ggttatggtc
    42001 atcaaatcaa cggttcccgt tggttttacc gcagcgatgc ataagaaata tcgtactgaa
    42061 aatattattt tctctccgga attcctccgc gaaggtaaag ccctttacga taatctccat
    42121 ccgtcacgta ttgtcatcgg tgagcgttca gaacgcgcag aacgttttgc tgcgttgtta
    42181 caggaaggcg cgattaagca aaatatcccg accctgttta ccgactccac tgaagcagaa
    42241 gcgattaaac ttttcgctaa tacctatctg gcgatgcgcg tagcatactt taatgaactg
    42301 gatagttatg cagaaagttt aggtctgaat actcgtcaga ttatcgaagg cgtttgtctc
    42361 gatccgcgta ttggcaacca ttacaacaat ccgtcgtttg gttatggtgg ttattgtctg
    42421 ccgaaagata ccaagcagtt actggcgaac taccagtctg tgccgaataa cctgatctcg
    42481 gcaattgtcg atgcaaaccg cacgcgtaaa gattttattg ccgatgccat tttgtcacgc
    42541 aaaccgcaag tggtgggtat ttatcgtctg attatgaaga gcgggtcaga taacttccgt
    42601 gcgtcttcta ttcaggggat tatgaagcgt atcaaggcga aaggcgttga agtgatcatc
    42661 tacgagccgg tgatgaaaga agactcattc ttcaactctc gcctggaacg tgatctcgcc
    42721 accttcaaac aacaagccga cgtcattatc tccaaccgta tggcagaaga gcttaaggat
    42781 gtggcagata aggtctacac ccgcgatctc tttggcagcg actaacatct tgttatcagg
    42841 gctatttacg ccctgattgt cttttgttac ctccgcaata attcattatt tttatcactt
    42901 atcctatagc attcacgggg attatcgcta aactatgcgg acttggaaat ttccgtcagt
    42961 tagggtaatg atgagagtag aaaataataa tgtttctggg caaaaccatg acccggaaca
    43021 gattgatttg attgatttac tagtgcagtt gtggcgtggc aagatgacaa ttatcatttc
    43081 cgtcattgtg gctattgccc tggctattgg ttatttggca gtagcgaagg agaaatggac
    43141 gtcaacagca attatcactc agcccgacgt ggggcaaatt gctggctata acaatgccat
    43201 gaatgttatc tatggtcagg ctgcaccgaa agtatcggat ttgcaggaga cgttaattgg
    43261 tcgcttcagt tctgccttct ctgcattagc agaaacgctg gataatcagg aagagccaga
    43321 aaaacttacc atcgaacctt ctgttaagaa ccagcaatta ccattgactg tttcttatgt
    43381 tgggcaaact gcagagggcg cacaaatgaa gttggcccaa tacattcagc aagttgatga
    43441 taaagtgaat caagagctag aaaaggatct caaggacaac attgctctgg gacggaaaaa
    43501 cttgcaggac tctttaagaa cccaggaagt ggtcgcgcag gagcagaaag atctgcgtat
    43561 ccgtcagatt caggaagcgt tgcagtatgc gaatcaggcg caggtgacaa agccacaggt
    43621 tcagcagact gaagatgtga cgcaagatac gttgttcctt ctagggagcg aagcgctgga
    43681 gtcgatgatt aagcatgaag cgactcgtcc gttggtgttc tcaccaaact actatcagac
    43741 acgtcaaaac ctgttggata ttgaaaaatt aaagtttgat gatcttgata ttcatgctta
    43801 ccgctatgtg atgaaaccga cgttacctat tcgtcgcgat agtccgaaaa aggcaatcac
    43861 cttgattctg gcagtgcttc tgggcggcat ggttggcgcg gggattgtgt tggggcgtaa
    43921 cgctctgcgt aattacaacg cgaagtaata ttattgtgca tttaagagaa acgggcaggg
    43981 tggtgacacc atgcccgttt tttttgccgg atgcgatgct ggcgcatctt atccggccta
    44041 cgtgtgttga gataatgtgt aggcacgata agtttgcgca tcgggcaatg gctccgggtg
    44101 tgacaacaac atcacacctg ctcagcccgt aactcactga tgccgtttac gcaggttctc
    44161 aattactgtc gttaaatcca gatcctgatc ctgcaacagc accagcaggt gatacatcaa
    44221 gtcagatgcc tcgttggtca gctcaaagcg atcatgtacc gttgctgcca gtgcggtttc
    44281 cacgccttct tcgcccactt tctgcgcaat gcgtttagtg ccgctggcat acagtttggc
    44341 ggtgtaggag gtttccgggt cggcatattt acgttcggcg agcagttgtt ccagttgata
    44401 caggaacagc cactggtgag cggtgttgcc gaagcagcta ctggtgcctt tgtggcaggt
    44461 tggcccgatg gaattcgcca gcaccagtaa tgtgtcgttg tcgcagtccg gggcaatact
    44521 taccacattg agaaaattgc ccgaggtttc gcctttggtc cacagtcgct gtttagtgcg
    44581 cgagaagaag gtgactttac cgctttcgat ggttttgtct aaggcttccg ggttcatgta
    44641 gcccagcatt aacacttcgc cagataccgc gtgttgcaca atcaccggca tcagaccgtc
    44701 ggttttttcc cagtccagtt cgcgacgttg ttgttctgtt aacatatcct gatctccacg
    44761 ccctgtgttg ccaggtacgc ttttaattcg ccaatattga ttatttgttt gtggaatacg
    44821 gaagctgcca gcgcaccgtc aacgtcggca tcgcggaagg cttccaggaa gtgttccatg
    44881 gtgcccgcgc caccggaggc aatcagcggg acgtggcaaa cttcacgcac ttttttcagc
    44941 tgcttgaggt cgtaaccgtt acgcacgccg tcctgattca tcatgttgag gacgatttct
    45001 ccggcgccgc gtttttgcac ctcctctacc cagtcgagcg tttcccattg agtgacgcgg
    45061 gtgcggcttt catcgccggt atattgattt acatgatatt taccggtttc ggcgtcgtac
    45121 caggtatcaa taccaaccac aatacactgc acgccaaagc gatcggccag gcgagtaatt
    45181 aatgttgggt ctgccagcgc aggggagttg atggaaattt tatccgcgcc aaaggaaaga
    45241 attttcgcgg catcttccag agacttaatc ccacccgcca cacaaaacgg aatgtcgatc
    45301 acttccgcca cgcgagatac ccagctttta tctaccacac ggccatcgct ggaagcggtg
    45361 atatcgtaga acaccagttc atctgcacct tcttcggcgt agcgttttgc cagtggcacg
    45421 atatcgccaa tgatttcatg gttgcgaaac tgtacgcctt tcaccacctg accatcacga
    45481 acgtcgagac acgggattat gcgttttgcc agcatgcgat ggcctccttc acggtgaatt
    45541 taccttccag taatgcacga ccaactatta cgccgcgcac gccagtgcca cgtagggcag
    45601 caacatcgtt aatgtcgcca atgccgccgg aggactgaaa cgccacttgc ggatatctgg
    45661 cacacacttc ttcatataaa gagacgttag atcccgccag cgtgccgtcg cgcgagatat
    45721 cggtacacaa cacatgtttc aggccgacgg gcagataggt ttccaccagt tgttccagtg
    45781 aaacgcccga gttctcttgc cagccgctga ctgccacttg cttgttgcct tgctcgtcaa
    45841 tacggacatc cagtgccagc accagcgcat cggcaccgaa acgttcaaac cagcctttca
    45901 ctacgtccgg tgatttcact gcggtggaac cgaccaccac gcgcgcaacg ccagcttcca
    45961 gtaacgccgc cacgtcttct tcactacgaa caccgccgcc aacctgcacc gggacgttaa
    46021 cgcccgcgac cagggtttta atcagcggga tttgacgctt agccggatct tttgccccgg
    46081 tcagatccac caggtgcaac acttcggcac cttgcgcagc gtaatcctgt aagcgcggca
    46141 gcgggtcgtt accgtaatcg cgttgtttgc cgtaatcgcc ctgatggaga cgcaccacgg
    46201 tgccgtcgat taaatctaat gccggaataa tcatcacatc tccaggaagt ttttcagcaa
    46261 cttagcgcca gcggcaccag aacgctccgg gtggaactgc acgccgtaga agttatcttt
    46321 ttgtaccgcc gcggtgaacg gttcgccgta attacactgg gcgatggtcc acggattgac
    46381 tggcattgcg tagctgtgaa caaagtaaaa gtacgcgccg tcttcaatcc cctgaaacag
    46441 gcggttgcct gcctgcggat aaacgcggtt ccagcccata tgtggcagtg gcagaccaaa
    46501 gtcggtcatt ttcggcacgt cttcgtcgat gatgcccagc aagtcgacgc cattgctctc
    46561 ttcgctgtgt cgtcccagga gttgcatccc taagcagatg cccagcaccg gttgggtaca
    46621 ggctttgatg agatcaaaca gctcgcgctc acgcacctga tccatcgctg cttgtgcggt
    46681 gccgacgccg ggtaaaaaca gtttatcggc cagcaacaca acgtccgggt cacggctgac
    46741 tttgggttca taaccgtggc gcgcaatggc agacttcaca gagttcaggt tggcgcagcc
    46801 agtatcaagg atcaccacgt tcattacagc actcctttcg acgagggcag ggtgtcgcct
    46861 tcgacgcgga tggcctggcg cagggtacga ccaaaggctt tgaacaggct ttctacacgg
    46921 tgatgatcgt ttttaccttt ggttttcagg tgtagtgtca cgcccatggt gtaagagagc
    46981 gaacggaaga agtgttcgat catttcagtg ctgagatcgc ccacacgctg gtaggtaaac
    47041 tcggctttat attccaggtg cgggcgacca gagatatcca gtgcgcagcg ggcaaggcat
    47101 tcgtccatcg gcagcacaaa accaaagcgg caaataccgc gtttgtcgcc gagggcaatt
    47161 tttaacgctt cgcccagcgc caggccggta tcttcgacgg tgtggtgatc gtcgatatag
    47221 aggtcgcctt tgacattgat ttccatgcgg aaaccgccgt gggtggcgat ctgatccagc
    47281 atgtgatcaa agaagccaac gccggtgtta atcttgctgc caccttcgcg atccagccac
    47341 acctggacgt caatctgcgt ctctttggtg ttgcgcacta catgggcgta acggtctcgt
    47401 ttagtgagtt gctcgccaat cattggccag ttcagggttt cgcggtcgta gcgtaaacca
    47461 ttaatgccca tgttttccgc cagttgaatg tcggtcgcgc gatcgccaat tacataactg
    47521 ttggcacgat ccatcgcttg ctcagccaga taaccctcca ccagttttac tttcggctta
    47581 cggcagtcgc actcatcggc gggcaggtgc ggacaaatca gcacttcatc aaactgcacg
    47641 ccttgcgagg tgaagatctg catcatcagg ttgtgcgggc catcaaaatc cgcttgcggg
    47701 aaactttgtg ttcccagacc atcctgatta gtgatcatca ccagcttgta gcccgctttt
    47761 tgcagcttca gcaattccgg gatcacgccc ggttcaaagg cgagtttgtc aaaacggtcc
    47821 acctgaaaat cactcggcgg ttcgctaatc agggttccat cgcgatcgat aaaaagatac
    47881 ttctgactca tcaaacttgc tccgcacgta aggcgtcaat gacgcgctgg ctttcttcac
    47941 gggttccgac ggtaattcgc aggcagccgc ttaaagaggg ttgtttattc tgatcacgta
    48001 agataatgcc ctgatcccac aaagatttaa acactgcact ggaggcttta aagcgcgcta
    48061 gaatatagtt ggtttcagag tcgaaaacct gctccacgca ggggatctct ttcagtgcgg
    48121 caatcaggta ttcgcgttct gtaataattt gcgccacccg ttcgcgcatg gcgacgattc
    48181 cctgcgggct taacgcctgt gcggcaatgt cggcaaccgg cgtcgagagc gggtaggggg
    48241 ggatcacttt catcagcagg ttgatgactt cttcgtttgc cagcgtaaat ccgcaacgaa
    48301 gccccgccaa cgcgaaggct ttcgaaaggg tacgcaaaat cgccaggtgc ggatattccg
    48361 ccagccagcc agctaacgat gcctgcgggc aaaactcgat ataagcttca tcggcaacca
    48421 caatcgcctt accgcgggtt agctccagca gggggcgaaa atcctgcgga ttgatcagtt
    48481 gcccggtggg gttgttgggg ctgcaaacat agaccacttt tacgccgtcc agcttgtcgg
    48541 aaatgccctg taagtccagt tgccagtttt ccagcgtcgg cactgtgcgg cattcgacac
    48601 caatggtttc agcgctgacg ctgtacatgc cgtacgttgg cgggcagtag aggatggcgt
    48661 ctttacccgg ttcgcaaaaa gcgcggatca gcagttcaat accttcgtcc gcgccacggc
    48721 tgaccagcac ctgttccggt tttacgcctg catattgcgc gtaattttca atcaccgctt
    48781 ttggctggca ttccgggtag cggttgagcg tttgctgagt aagctgaaac tccacggctg
    48841 tgggatattc gttggcgttc agccagacgt cgccgttacc gcccagacga cgcgccgact
    48901 gatacggcgt caggttgcgg acgttttcac gcgctaaatc agtaatagtc acggtgctca
    48961 tgcttgctcc ttaaggacgt taacacgcaa agtaacggca tttttgtggg cggtcaggcg
    49021 ctcggcggcg gccagtgttt caatggtcga agccagcgcg gagaaacctt ctttcgacag
    49081 ttcctgtacg gtcatgcgtt tctggaaatc tgccagcccg aggctggaac aggtggcggt
    49141 gtaaccgtaa gtcggcagca cgtggttggt gccggaggcg tagtcgcctg ccgattccgg
    49201 tgaccagtca ccaagaaata ccgaaccggc gctggtgatg ccatcgacca gttcgcgggc
    49261 gttgcgggtc tgaatgatca ggtgctccgg gccgtactgg ttggagatct ccacgcactg
    49321 cgctaaatct ttagtcacga tcaggcggct ggcgttcagt gcctggcggg tggtttcggc
    49381 acgcggcagt tctgccagtt ggcgttcgac ggcctcggca acgcgacgcg ccatatcagc
    49441 atcgggggtc agtaaaatta cctgtgagtc agggccgtgt tcagcctgag aaagcaaatc
    49501 agaagccaca aaatccggcg tagcgccgct gtcagcaatc accagcactt ccgacgggcc
    49561 tgcgggcata tcgatcgccg caccgtccag acgctggctt acctggcgtt ttgcttcggt
    49621 gacaaaggcg ttacccggcc cgaagatttt gtccactttc ggcacagatt ccgtaccaaa
    49681 cgccagcgcg gcaatggcct gtgcgccgcc gacgttaaac acgtcctgca catcgcacag
    49741 ctgcgccaca taaagaattt catcggcaat cggcggcggt gagcacagca ccactttttt
    49801 acagcccgca atacgcgccg gagttgccag cattaatacc gttgagaaga gcggggcgga
    49861 gccgcccgga atatacaaac ccactgaagc caccgggcgc gtgacctgct ggcaacgcac
    49921 gcctggctgc gtttctacat ctaccggcgg cagtttttgc gcagtgtgga aggtttcaat
    49981 actctttact gccaccgcca tcgcctgttt tagctcgtcg ctcaggcgtt cgctggcggc
    50041 ggcgatctca tcagcagaca ccttcagcgc ggtaaccgtg gttttatcaa acttcgcgct
    50101 gtattcacgc agggcctcat cgccacgcgt tttcacgtta tcgagaatat cgttaacggt
    50161 gcgggtaatg ctttcagaag cggagatcgc cgggcgtatt aacagctggc gttgttgctc
    50221 cgcagtacag ctattccagt caatgattgt gttaaagctc atggcgatca ctccatcatc
    50281 ttctcaatcg gcaggaccag gattgaactg gcacccagtg ctttcagttt ttccatcgtt
    50341 tcccagaaca gggtttcgct actcaccatg tgcatcgcta cgcgctgttg atcacccgcc
    50401 agcggcagaa tggttgggcg ttcggcacct ggcagcaggg cgatgacttc atccaggcgt
    50461 tcggtcggtg cgtgcatcat gatgtatttt gattcgcgcg cctggatcac accctgaata
    50521 cgggtcagta gcttgtcgat cagttgctgt ttagattctt ccatttcgcc atcgcgctgg
    50581 atcaggcagg ctttcgagcg gtagataact tcgacttcgc gcaggccgtt agcttccagc
    50641 gtggcaccgg tggaaaccag atcgcaaatc gcatccgcca gcccggcacg cggcgcaact
    50701 tcgacagaac cgttcagtaa gcaggattta aaagagatac ctttctggtc gagataacgc
    50761 ttgagcaggt gaggataaga ggtggcgata cgtttaccgt ttaaggagag cggaccgtcc
    50821 caggcttcat caaccggcgt tgccagcgaa aggcggcagc cgccgaaatc cagacgacgc
    50881 agggtaaagt agcgcggatc ttcaccctgg gcgcggcggt taagcagctc ttcttccagc
    50941 acgttttcgc cgataatccc aaggtctacc acgccgtcca ttaccagacc gggaatgtca
    51001 tcgtcacgca cgcgcagaat atcaatcggc atgttttctg ccatcgcgat caggcgctgg
    51061 gtgtgaagat tgattttaat gccacagcgc gccagtaatt cgcgtgagtc atcacttaaa
    51121 cggccggatt tctgcatagc tatgcgtaaa cgagtgttgt ctgtcatttt cttattcctc
    51181 tttaaacctg tctgaaccgt taacaaccgc gcaataaaaa agcccccgga aggtgatctt
    51241 ccgggggctt tctcatgcgt tcatgcacca ctggaagatc tgaatgtctt ccagcacaca
    51301 tcgcctgaaa gactagtcag gatgatggtg atgatggtgg tgtttaaatt gaacgcgtgt
    51361 cataaaaatc tctgtgaatg tttattcaac tgatgtcttt taacctaaac cactttcacg
    51421 ttagaaagca agggcttttt tatatatttt atggcgatgg atttgtttaa tgaatgagcg
    51481 gggaaaggag ggggctaaga ggactgtcct ctaacttgac agcaccaaaa agagcaaagt
    51541 ctgatttttc gtctcgctgg caatgtcttg atctacaaac taattaataa atagttaatt
    51601 aacacctatc attgtacaat gaactgtaca aaataggaga tttacatgcg tacaattagc
    51661 tacagcgaag cgcgtcagaa tttgtcggca acaatgatga aagccgttga agatcatgcc
    51721 ccgatcctca ttactcgtca gaatggagag gcttgtgttc tgatgtcact cgaagaatac
    51781 aactcgctgg aagagacggc ttatctactg cgttcccccg ctaacgcccg gagattgatg
    51841 gactcaatcg atagcctgaa atcaggcaaa ggaacggaaa aggacattat tgagtgaaac
    51901 taatctggtc tgaggaatca tgggatgatt atctgtactg gcaggaaaca gataagcgaa
    51961 ttgttaaaaa gatcaatgaa cttatcaaag atacccgcag aacaccattt gaaggtaagg
    52021 ggaaaccaga acccctgaaa cataatttgt caggtttctg gtcccgacgc attacagagg
    52081 agcaccgtct ggtatacgcg gttactgatg attcactgct cattgcagca tgtcgttatc
    52141 attattgaac agattcattc atagaatgaa ttgtcagttt tgatacgcta gcgtagcctt
    52201 atagatctaa ggtgtatcag gagataacgg atgaaaaagg tcgcaattgt cgggttaggg
    52261 tggttaggta tgccgctggc gatgtcactt tcagcgcgag gctggcaagt caccgggagt
    52321 aaaaccacac aagatggtgt cgaagcggcc cgaatgagtg gcattgatag ctatctgctt
    52381 cgcatggagc ctgagttagt ttgcgattct gacgatctgg atgcccttat ggatgccgat
    52441 gcgctggtca ttacgcttcc ggcacgtcgt agcggccccg gcgatgagtt ctatttacaa
    52501 gcggtacaag agttagtgga tagcgcgctg gcccatcgta ttccccgaat tatttttacc
    52561 agctcaacat ctgtctatgg cgacgcgcaa ggcacggtta aagaaactac cccgcgtaat
    52621 ccggtaacca acagtggacg agtattagaa gaactcgaag actggctgca caatttaccc
    52681 ggtacttcgg tcgatattct gcgtcttgcg ggcctggtcg gaccgggacg tcatccggga
    52741 cgcttctttg ctggaaaaac cgcccctgat ggtgaacatg gtgttaattt agtccattta
    52801 gaagatgtta ttggcgctat cactctgttg ttacaggcac ctaaaggcgg acacatctat
    52861 aatatatgtg cgccagctca cactgcgcgt aatgttttct atccgcagat ggcccgttta
    52921 ctggggctgc aacctccgca gttcagaaat agtctggaca gcggcaaagg taggattatt
    52981 gatggcagtc ggatttgtaa tgaactggga tttgaatacc agtatcccga tccgctggta
    53041 atgccgctgg agtaaacgat cacgccagca ggacgccgca tgcactgcaa gggggcgtgt
    53101 atgaaaccac tgctggatgt gttgatgatc ctcgatgcca tagaaaaaga gggcagtttt
    53161 gcggcggcgt cagccaaact ctacaagacg ccgtccgctc tgagttacac cgttcacaag
    53221 ctggaaagcg acctaaatat tcaattgctt gatcgtagcg gacaccgcgc taaattcacc
    53281 cgcaccggaa aaatgttatt agagaaaggg cgggaagttc tgcataccgt gcgagaactg
    53341 gaaaagcagg cgatcaaact gcatgaaggc tgggaaaacg agctggtgat tggcgtggac
    53401 gacacgtttc ctttttctct gcttgcgcct ctcattgaag ctttttatca acatcatagc
    53461 gtgacgcgcc tgaagtttat caatggcgta ctcggtggtt cctgggatgc cctgactcag
    53521 gggcgagcgg atattatcgt tggcgcgatg catgagccac catcttccag tgagtttggt
    53581 ttctcgcggt tgggcgatct tgaacaagtc tttgcggtcg cgcctcatca tccgttagcc
    53641 caggaagaag aaccgttaaa ccgtcgtatc attaaacgat atcgggcgat tgtggtgggg
    53701 gacactgcgc aggcaggcgc ttctacggcc tcacaacttc tcgacgaaca agaagccatt
    53761 accgttttcg attttaaaac caaactggag ctgcaaatta gcggccttgg ctgcggctat
    53821 ttaccacggt atctggcgca acgttttctc gatagtggcg cgttaatcga gaagaaagtg
    53881 gtcgcccaaa ctctctttga acctgtctgg attggctgga atgaacagac cgcagggctt
    53941 gccagcggct ggtggcgaga tgaaatttta gcaaatagtg cgatcgccgg tgtttatgca
    54001 aaatctgatg acggaaaatc agccatttaa agaaaaatta ttatgacaag cctctcattc
    54061 tcttgtcatt tcccccccat ttaggcacaa tgcgccgctg tcaaaaaatg actaaaaacc
    54121 gacgtttaat tagcgtcggt tattttttgc ttcaaaccaa tcattcatac caagaggccg
    54181 ggcttcgtac cggatagata tttactaaaa atcgacagtt gttgtcgctg aggaatccaa
    54241 aaaaatgggg caattttttg cttacgcgac ggttatcacc gtaaaggaga ataaccatgt
    54301 cgcataacgt tactccaaac acctctcgcg tggaattgcg taaaacgctt acgttagttc
    54361 cggttgtaat gatgggtcta gcctatatgc agccgatgac gctgtttgat acatttggta
    54421 tcgtttcagg cctcacggat ggtcatgtgc cgacagccta tgcgttcgca ttgattgcga
    54481 tcctgtttac ggctctgagc tacgggaagc tggttcgccg ctatccttct gctggctctg
    54541 catacactta cgcccagaaa tccattagcc cgactgtcgg ctttatggtg ggttggtctt
    54601 ctctgctcga ctatctgttc gcgccgatga tcaacattct gctggcgaaa atctattttg
    54661 aagctctggt gccttccatt ccatcgtgga tgtttgtggt ggcgctggtg gcctttatga
    54721 ccgcctttaa cctgcgtagt ctgaaatccg tagcgaactt caacactgta atcgtcgtgt
    54781 tgcaggtagt gctgatcgcg gtgattctgg gcatggttgt ttatggcgta tttgaaggtg
    54841 aaggcgctgg tacgctggcg agcacgcgtc cgttctggtc tggcgatgcg catgttatcc
    54901 cgatgattac cggggcgaca atcctgtgct tctcctttac cggctttgac ggcatcagta
    54961 atctgtcgga agaaaccaaa gatgcagagc gcgtgatccc gcgtgcgatt ttcctgaccg
    55021 cgctgattgg cggcatgatc ttcatctttg caacttactt cctgcagctg tacttcccgg
    55081 atatctctcg ctttaaagat ccggatgcgt cacagcctga aatcatgctg tacgttgcgg
    55141 gtaaagcgtt ccaggttggc gcgctgatct tctccaccat taccgtactg gcgtccggta
    55201 tggcggcgca cgcaggcgta gcgcgtctga tgtacgtaat gggtcgtgac ggcgtgttcc
    55261 cgaaaagctt cttcggttat gtacacccga aatggcgtac tccggcgatg aacatcgtcc
    55321 tggttggcgc gattgccctg ctggcaatca acttcgacct ggtaatggca acggcgctga
    55381 ttaactttgg tgcgctggtg gcgttcacct ttgttaacct gtcggtcatc tcgcagttct
    55441 ggatctgtga gaagcgtaac aagacgctga aagatcactt ccagtatctg ttcctgccga
    55501 tgtgtggtgc gctgaccgtt ggtgcgctgt gggttaacct ggaagaaagc tcaatggttc
    55561 tgggtctgat ctgggctgct atcggtctga tttacctggc ttgcgtcacc aagagcttcc
    55621 gtaatccggt tccgcagtac gaagacgtag cataatcatc ttttctaata ataaacagga
    55681 gtcgaatgaa catttggctc cggttttttt attctttttt atcgaaatcc tcattaataa
    55741 ccatttgttc taatttagag aatttggtt
//
LOCUS       DADLSB010000009        52860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.009, whole genome shotgun sequence.
ACCESSION   DADLSB010000009 DADLSB010000000
VERSION     DADLSB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.009"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..605
                     /locus_tag="JN331_000547"
     CDS             <1..605
                     /locus_tag="JN331_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258777.1"
                     /translation="TIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTNCNS
                     VRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELV
                     FEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYS
                     FQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(643..1542)
                     /gene="nhaR"
                     /locus_tag="JN331_000548"
     CDS             complement(643..1542)
                     /gene="nhaR"
                     /locus_tag="JN331_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY7258778.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1608..2774)
                     /gene="nhaA"
                     /locus_tag="JN331_000549"
     CDS             complement(1608..2774)
                     /gene="nhaA"
                     /locus_tag="JN331_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY7258779.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3303..3512
                     /gene="mokC"
                     /locus_tag="JN331_000550"
     CDS             3303..3512
                     /gene="mokC"
                     /locus_tag="JN331_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY7258780.1"
                     /translation="MLNTCRVPLTDRKVKGKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            complement(3616..4746)
                     /gene="dnaJ"
                     /locus_tag="JN331_000551"
     CDS             complement(3616..4746)
                     /gene="dnaJ"
                     /locus_tag="JN331_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY7258781.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4835..6751)
                     /gene="dnaK"
                     /locus_tag="JN331_000552"
     CDS             complement(4835..6751)
                     /gene="dnaK"
                     /locus_tag="JN331_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY7258782.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            7128..7532
                     /locus_tag="JN331_000553"
     CDS             7128..7532
                     /locus_tag="JN331_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY7258783.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7558..8271
                     /gene="msyB"
                     /locus_tag="JN331_000554"
     CDS             7558..8271
                     /gene="msyB"
                     /locus_tag="JN331_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY7258784.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLHGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8420..8986
                     /gene="satP"
                     /locus_tag="JN331_000555"
     CDS             8420..8986
                     /gene="satP"
                     /locus_tag="JN331_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY7258785.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNITGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(9021..9608)
                     /gene="mog"
                     /locus_tag="JN331_000556"
     CDS             complement(9021..9608)
                     /gene="mog"
                     /locus_tag="JN331_000556"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY7258786.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLASALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9723..10676)
                     /gene="tal"
                     /locus_tag="JN331_000557"
     CDS             complement(9723..10676)
                     /gene="tal"
                     /locus_tag="JN331_000557"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7258787.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10955..12385
                     /locus_tag="JN331_000558"
     CDS             10955..12385
                     /locus_tag="JN331_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAY7258788.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGFWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            12455..13231
                     /gene="yaaA"
                     /locus_tag="JN331_000559"
     CDS             12455..13231
                     /gene="yaaA"
                     /locus_tag="JN331_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY7258789.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13384..13680)
                     /locus_tag="JN331_000560"
     CDS             complement(13384..13680)
                     /locus_tag="JN331_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7258790.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13894..15180)
                     /gene="thrC"
                     /locus_tag="JN331_000561"
     CDS             complement(13894..15180)
                     /gene="thrC"
                     /locus_tag="JN331_000561"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY7258791.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(15181..16113)
                     /gene="thrB"
                     /locus_tag="JN331_000562"
     CDS             complement(15181..16113)
                     /gene="thrB"
                     /locus_tag="JN331_000562"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY7258792.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(16115..18577)
                     /gene="thrA"
                     /locus_tag="JN331_000563"
     CDS             complement(16115..18577)
                     /gene="thrA"
                     /locus_tag="JN331_000563"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY7258793.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVFSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLDGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(18658..18723)
                     /gene="thrL"
                     /locus_tag="JN331_000564"
     CDS             complement(18658..18723)
                     /gene="thrL"
                     /locus_tag="JN331_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY7258794.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18937..19623)
                     /locus_tag="JN331_000565"
     CDS             complement(18937..19623)
                     /locus_tag="JN331_000565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7258795.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(20023..20163)
                     /locus_tag="JN331_000566"
     CDS             complement(20023..20163)
                     /locus_tag="JN331_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258796.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20259..20975
                     /gene="arcA"
                     /locus_tag="JN331_000567"
     CDS             20259..20975
                     /gene="arcA"
                     /locus_tag="JN331_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY7258797.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(21034..22386)
                     /gene="creD"
                     /locus_tag="JN331_000568"
     CDS             complement(21034..22386)
                     /gene="creD"
                     /locus_tag="JN331_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY7258798.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22444..23868)
                     /gene="creC"
                     /locus_tag="JN331_000569"
     CDS             complement(22444..23868)
                     /gene="creC"
                     /locus_tag="JN331_000569"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY7258799.1"
                     /translation="MRIGMRLLRGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSVNKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPSEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23868..24557)
                     /gene="creB"
                     /locus_tag="JN331_000570"
     CDS             complement(23868..24557)
                     /gene="creB"
                     /locus_tag="JN331_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY7258800.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24570..25043)
                     /gene="creA"
                     /locus_tag="JN331_000571"
     CDS             complement(24570..25043)
                     /gene="creA"
                     /locus_tag="JN331_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY7258801.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25254..26123
                     /gene="robA"
                     /locus_tag="JN331_000572"
     CDS             25254..26123
                     /gene="robA"
                     /locus_tag="JN331_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY7258802.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(26120..26767)
                     /gene="gpmB"
                     /locus_tag="JN331_000573"
     CDS             complement(26120..26767)
                     /gene="gpmB"
                     /locus_tag="JN331_000573"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY7258803.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26819..27340
                     /gene="yjjX"
                     /locus_tag="JN331_000574"
     CDS             26819..27340
                     /gene="yjjX"
                     /locus_tag="JN331_000574"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY7258804.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(27425..27751)
                     /gene="trpR"
                     /locus_tag="JN331_000575"
     CDS             complement(27425..27751)
                     /gene="trpR"
                     /locus_tag="JN331_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY7258805.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27841..29778)
                     /gene="sltY"
                     /locus_tag="JN331_000576"
     CDS             complement(27841..29778)
                     /gene="sltY"
                     /locus_tag="JN331_000576"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY7258806.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGHSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29989..31656
                     /gene="ettA"
                     /locus_tag="JN331_000577"
     CDS             29989..31656
                     /gene="ettA"
                     /locus_tag="JN331_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY7258807.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31964..33196)
                     /gene="nadR"
                     /locus_tag="JN331_000578"
     CDS             complement(31964..33196)
                     /gene="nadR"
                     /locus_tag="JN331_000578"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY7258808.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(33217..34599)
                     /gene="radA"
                     /locus_tag="JN331_000579"
     CDS             complement(33217..34599)
                     /gene="radA"
                     /locus_tag="JN331_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY7258809.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34648..35616)
                     /gene="serB"
                     /locus_tag="JN331_000580"
     CDS             complement(34648..35616)
                     /gene="serB"
                     /locus_tag="JN331_000580"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY7258810.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LQMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35722..36366
                     /locus_tag="JN331_000581"
     CDS             35722..36366
                     /locus_tag="JN331_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY7258811.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36394..37410
                     /gene="lplA"
                     /locus_tag="JN331_000582"
                     /pseudo
     CDS             36394..37410
                     /gene="lplA"
                     /locus_tag="JN331_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37442..37705
                     /locus_tag="JN331_000583"
     CDS             37442..37705
                     /locus_tag="JN331_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7258812.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37866..38585)
                     /gene="deoD"
                     /locus_tag="JN331_000584"
     CDS             complement(37866..38585)
                     /gene="deoD"
                     /locus_tag="JN331_000584"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY7258813.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38642..39865)
                     /gene="deoB"
                     /locus_tag="JN331_000585"
     CDS             complement(38642..39865)
                     /gene="deoB"
                     /locus_tag="JN331_000585"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7258814.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39917..41239)
                     /gene="deoA"
                     /locus_tag="JN331_000586"
     CDS             complement(39917..41239)
                     /gene="deoA"
                     /locus_tag="JN331_000586"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY7258815.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(41366..42145)
                     /gene="deoC"
                     /locus_tag="JN331_000587"
     CDS             complement(41366..42145)
                     /gene="deoC"
                     /locus_tag="JN331_000587"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY7258816.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42403..43944
                     /locus_tag="JN331_000588"
     CDS             42403..43944
                     /locus_tag="JN331_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY7258817.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDEIIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43916..44779
                     /gene="yjjW"
                     /locus_tag="JN331_000589"
     CDS             43916..44779
                     /gene="yjjW"
                     /locus_tag="JN331_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY7258818.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNGCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44993..45775)
                     /locus_tag="JN331_000590"
     CDS             complement(44993..45775)
                     /locus_tag="JN331_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7258819.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45772..46845)
                     /locus_tag="JN331_000591"
     CDS             complement(45772..46845)
                     /locus_tag="JN331_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY7258820.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46967..47128)
                     /locus_tag="JN331_000592"
     CDS             complement(46967..47128)
                     /locus_tag="JN331_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY7258821.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47255..47860)
                     /gene="osmY"
                     /locus_tag="JN331_000593"
     CDS             complement(47255..47860)
                     /gene="osmY"
                     /locus_tag="JN331_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY7258822.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48253..49842)
                     /gene="prfC"
                     /locus_tag="JN331_000594"
     CDS             complement(48253..49842)
                     /gene="prfC"
                     /locus_tag="JN331_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY7258823.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49933..50610)
                     /gene="yjjG"
                     /locus_tag="JN331_000595"
     CDS             complement(49933..50610)
                     /gene="yjjG"
                     /locus_tag="JN331_000595"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY7258824.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50625..51071)
                     /gene="rimI"
                     /locus_tag="JN331_000596"
     CDS             complement(50625..51071)
                     /gene="rimI"
                     /locus_tag="JN331_000596"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7258825.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(51040..51453)
                     /gene="holD"
                     /locus_tag="JN331_000597"
     CDS             complement(51040..51453)
                     /gene="holD"
                     /locus_tag="JN331_000597"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY7258826.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51556..52587
                     /gene="rsmC"
                     /locus_tag="JN331_000598"
     CDS             51556..52587
                     /gene="rsmC"
                     /locus_tag="JN331_000598"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY7258827.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12593 a        14299 c        13235 g        12733 t
ORIGIN      
        1 tcaccattcc atactctatc gcatctgtat cagatagttc caacaccgca gatatttttc
       61 caggaacgcc ttttcctttg ttgttcgaaa ttgaagtgag ttcatgtaca gggaataaca
      121 ctaattgtaa tagcgtccgc tttaatttta atttaaatac tattttacag gtaaatttaa
      181 tgacctgtgg ttttgaagat caatccatcg acactggacg atttgtactg tctcgtatca
      241 ataatgaaaa ttatcagttt catcatataa gatttgactg catccagggc gagcaaggtg
      301 aattagtttt tgaacccagt gacgttgaat attactttga accagtgacg atacaggagt
      361 caggaaccag catattaaaa aatgagttag aaaacagtga ggacggtgcg ggtcaaatag
      421 gctttcaact ctccaatgat ggtacgaatg aaatccagta tggtaaaagc aattactact
      481 cttttcagca tccccatgaa ggaagtaatc agattcctct atttattcgc ccgcgtacat
      541 atggaaacaa tgtctcaagc gggcaaataa tgagccgagt caaaatcgtg gtaatgtaca
      601 actaatctat ctcccccacc ttaactttgg gggagctgcc gattaacgca ccgctggact
      661 aaaaagcgca gaataatccg tattgcagat tcgctgtacc gccgggtgct gaatcatccg
      721 ctcagcaaaa atagcatggt actcttccat cacattctcg acgcgaccaa tttctacgac
      781 agttttatcg gcataaaagt catatgcata aagcgttggg gcaacgaaga ttgcattgtg
      841 catcgcacca aaagctttca tcaaagcggc atcatcaaac tcgccgagga tttctacgtt
      901 taatccctgg gagttaaacc agttaagcaa tttgcgccct aacattgaac gtcgcccagg
      961 aatcaaaagt cgccgttctt ccagacaagc cgggaacggt ttttctggtg gtggatttgt
     1021 acaccagaaa ctcacgccac attcgccaat tctcacggag aacaggcctt cctgctgcgt
     1081 agagtctatc ggacagtcag aaatgatcat atccagttta tgctgactta attgctccag
     1141 cagcatttcg tgggtggatt cgaagcagcg aagatgaatg ggctcgcctt ctaccactgc
     1201 ggcgttaagt acgctactga ccaggcgttt ggaaagtgca tcagccacgc caacgtcaaa
     1261 caataaattg gattctttgc gatagttcac aatatccagc atttcctggc ttaaggtgaa
     1321 cattttatcg gcatagcgat agaccagttc tcccagctcg ctgggttcga gaccacgtcc
     1381 cttgcgttta aataatttgc cttgcaggcg ctcttccagc gctcgaatct gtccggtaat
     1441 ggtttgtgga gttaaataaa gcgcctccgc tgcgccaacc acggaacctt ctttatagac
     1501 atgccagaag taatacaagt ggttgtaatt gatatgagac atgctcattt ctctccctga
     1561 taacaatgaa aagggagccg tttatggctc cccggtaaac cgtcctgtca aactgatgga
     1621 cgcaaacgaa cgcgtaacca gctgtatcca attaccgccg aagagataga accgaccagg
     1681 ataccgagtt tcgcccagtt aatcagttct ggatctacgc taccaaaggc caggctggca
     1741 ataaagatag acatagtaaa accgataccg cacaggatcc ccaccaccat aatttgctga
     1801 taagtcgttc cctcaggcag atgcgccagt ttcaaacgca gcgccaacca gcagaacaga
     1861 ctaatcccca gcggtttgcc aatcagcaag ccagcgatga tccccaatgg cagaatggag
     1921 gtcaagccat ccagcgtgac gccttgcagt gaaacgccag cattagcaaa tgcaaacagc
     1981 ggcaaaatca gatacgccac ccacgggtgc aacacatgct ccagtcgctt cgctggagaa
     2041 cgcccatgct tctctttcaa aggaataaag aagccgacaa ttacccccgc cagagttgcg
     2101 tgaacccccg atttcaacac cgcagtccac aacaccacgc caacaagaat atagacgccc
     2161 gtgcggcgta caccacacag attcaatacc gcgagtaccg caattgctac agccgcgacg
     2221 ccaagagagg ccatcgataa gtcattagtg tagaacaatg cgatgataat gatggcccca
     2281 agatcgtcga taatagccag agccatcaaa aagatcttca gcgctaacgg aacacgactt
     2341 cccaacagcg ccagtacacc aagtgcaaaa gcaatgtcag tagccgccgg gatcgcccac
     2401 ccttcgcggg taatcggatc ggcatagtta aaagccagat agagtaatgc cggcacaatc
     2461 atcccaccaa tagcggcgat aactggaaat gcggcctggc gtaagctggc tagcgatcct
     2521 tgcatcagtt cacgtttaac ttccagaccg actaacagga aaaataccgc catcagcgcg
     2581 tcatttatcc ataacagcat gtttttgttg atttcgagtg aaccaacccg gagctgaacc
     2641 ggcgtctcca gaaagtcgtg ataccatcca ctggttgcgc cgctgttggc cataatcatc
     2701 gccaggatag cggcaatgat aagaataatg cctcccgagg catcactgct aaagaatcga
     2761 tgcagatgtt tcacttttta tttctctttc aggtgaatag atcgttttta ctattttacc
     2821 ccgcacgaat catcgttaaa atcagattat agtgtaggaa taattcgttt tttacgagct
     2881 attgaagaat ttgcgccatc agcggtgaga tgcgtttttg acggctgtaa gcgctcacga
     2941 gtgaccctaa gcccagataa tcatagctta aggtgaggat tcctgaggga ttaattagga
     3001 aaagtaataa ttaatttgtt gtcagtcggc gatgcttgtt aggccaatat ttcaaaaata
     3061 atccagcttg tcgtcaataa ttgcgatgaa gagataaatt aaagttaaat aattaaatca
     3121 ttaaaaaatt acgccacaaa aatgcgaggc gtcttcagga ttagaatcga taaaacaaaa
     3181 tgggttaaag agagcgatat aatagcggcg ggtgcttgag gctgtctgtc tcaggcatta
     3241 gctgaacggc agatagagaa aagccccgag tgatatttta ccatcaaccc gaggcctcct
     3301 atatgctgaa cacatgtaga gtgcctctta ctgaccgtaa ggtcaagggg aagagagcaa
     3361 tgaagcagca taaggcgatg attgtcgccc tgatcgtcat ctgtatcacc gccgtagtgg
     3421 cggcgctggt aacgagaaaa gacctctgtg aggttcacat ccgaactggc cagacggagg
     3481 ttgctgtttt cacggcttac gaatccgagt aagagcaacg gcgggggggg atccccgcca
     3541 ctttacaggt gctcgcatat cttcaacgca ccctattttt acccaggcct gcccacgggc
     3601 aggcttttgg ggaggttagc gggtcaggtc gtcaaaaaac ttcttcacac catcaaagaa
     3661 gctctttgag cgcgggctgt tgtgctcgcc ggttgggcca ccgaagcttt cttgcagctc
     3721 ttgcagcagc tgtttctgct tttcgttcag gcctaccggt gtttcgacga caacgcggca
     3781 cagcaaatca ccctgtgcgc caccgcggac agacttgacg cctttaccgc gcatacggaa
     3841 tagcttaccg gtctgggttt cgccaggcac tttcagtttg acgcgaccat caagggtcgg
     3901 tacttcgatt tcgccaccca gcgccgccat agcgaagttg atcgggactt cgcaatacag
     3961 gttgttgcct tcacgctcga aaatcgggtg ctgtttaacc tgaacctgaa cgtacagatc
     4021 gcctgccggt gcgccatgct cgcccgcttc accttcgccc gcaagacgga tgcggtctcc
     4081 agtgtccacc cctgccggga ttttaacgga cagcgttttg ctgcgctcaa cacgaccatg
     4141 accatgacat ttgttgcacg gatctttgat cagcgtaccg cggccctgac agtgtggaca
     4201 ggtctgctgt acagcgaaga atccctggcg catctgcacc tgaccagaac catgacaggt
     4261 cggacaggtc tgcggctgtg tacctggttt tgcaccgcta ccgtggcaaa cgtcacactc
     4321 ttccagagtc ggaatgcgga tctctttggt cacgccacgt acagcttctt cgagggtgag
     4381 ctccatgtta tagcgtaaat cagcaccgcg cgccgcacgt tgacgaccac gtccgccgcc
     4441 aaaaatatcg ccgaaaacgt caccaaaaat atcgctgaag tctgcgccgc cgccaaaacc
     4501 accgccgccc atgccacctt gctcaaacgc agcatgacca tactgatcgt atgccgcacg
     4561 tttttgcgag tcggtcagaa cttcataagc ttccttgatc tctttaaatt tcgcctcggc
     4621 ctctttgtca ccctggttac ggtccgggtg gtatttcatg gccagccgtt tgtaggcctt
     4681 tctgatttca cgctcttccg ctgttttgga aacgcctaaa atctcgtaat aatcttgctt
     4741 agccatcttt tttaaattgc ccctagatga atgcacgggc ggagaggaaa ttccccttcg
     4801 cccgtgtcag tataattacc cgtttatagg gcgattattt tttgtctttg acttcttcaa
     4861 attcagcgtc gacaacatcg tcatctttcg cgttgtttgc agaagcatca gcaccggcag
     4921 tctgctgctg ggcatgttgc tgctgggcga tttccatcag tttctgggaa acctgtgcca
     4981 gttcctgcat tttcgcttcg atagcggctt tgtcttcacc tttcagagca gtttccagtg
     5041 cagtcagcgc agactcgata gcagttttgt cgtcagccgg cagtttgtcg cctgcttctt
     5101 caacctgctt acgggtgctg tgcagcagat ggtcgccctg gttgcgagtc tgtaccagct
     5161 cttcaaactt acggtcagct tcggcgttag cttctgcgtc gcgtaccatt ttctggattt
     5221 catcttcgtt cagaccagaa gaagccttga tggtgatctt ctgctcttta ccgctgtttt
     5281 tatctttcgc ggaaacgtgc aggataccgt cagcatcgat atcgaaggta acttcgatct
     5341 gcggcatgcc gcgcggtgcc gggttgatac catctaggtt gaactgaccc agagatttgt
     5401 tatcagccgc acgtttacgt tcaccctgca gcacatggat ggttaccgca gactggttgt
     5461 cttcagcggt agagaacacc tggctgtgct tggtcgggat agtggtgttt ttcgcgatca
     5521 gcgtcgtcat cacaccgccc atggtttcga tacccagaga cagcggggta acgtccagca
     5581 gcagtacgtc ttttacgtca ccagtcagaa caccaccctg aacagcagca ccgattgcta
     5641 cagcttcgtc cgggttaacg tctttacgcg gctctttacc aaagaactca gcaactttct
     5701 tctgaaccat tggcatacga gtctgaccac caacgaggat aacgtcgtcg atatcagata
     5761 cggacaggcc agcatcctgc agtgcaactt tcagcggctc aatggaacgg tttaccagat
     5821 cttcaaccag gctttccagt ttcgcacgag tcactttgat gttcatgtgt ttcggaccgg
     5881 tcgcgtctgc agtgatgtat ggcaggttaa cgtcggtctg ctgagcggaa gacagttcga
     5941 ttttcgcttt ttctgccgct tctttcaggc gctgcattgc cagcggatcg ttgcgcaggt
     6001 caatgccctg atctttcttg aattcttcaa ccagatagtt gatcagacgg ctgtcgaagt
     6061 cttcaccacc caggtgggta tcaccgttgg ttgccagaac ttcgaaggtt ttttcgccgt
     6121 caacttcgtc gatttcgata atagaaatat cgaaagtacc accccccagg tcataaaccg
     6181 cgatagtacg gttgccagtg cctttgtcca gaccgtaagc cagcgcagct gcggtcggtt
     6241 cgttgatgat acgttttact tccagaccag cgatacggcc tgcgtctttg gttgcctgac
     6301 gctgagcatc gttaaagtat gccggtacgg tgataacagc ttcagttacc ggttcaccca
     6361 ggtaatcttc agcggttttc ttcatttttt tcagcacttc agcagaaatc tgcggcggtg
     6421 ccattttctg gcctttaact tcgacccatg cgtcgccgtt atcagcagca ataattttga
     6481 acggcatgat ggaaacatca cgctgtactt cttcgtcctg gaagcggcgg ccaatcaggc
     6541 gtttaatcgc aaacagggtg ttttgcgggt tcgtcactgc ctgacgttta gccggctgac
     6601 caaccagagt ttcaccatcc tgggtatagg caatgataga aggcgtggtg cgatcgcctt
     6661 cggcgttctc cagcacgcga ggagtggtgc catccataat cgctacacaa gagttggtag
     6721 tacccaggtc gataccaatt attttaccca tctaaacgtc tccactatat attcggtcat
     6781 catgtggttg tgagtctgta ataggggcga aacgtctggt ttcaactgcc caatttcatt
     6841 tttttgcaga ctcactcact gcggttgact actaaatggg gtcgtaaccc acgtcatcaa
     6901 gggggagatg caaaaatttt ttgtgctttt gcgcagaaat ttcgcgatat ggcaggacaa
     6961 gacgtaaaat tgacagcaat tttattcata taaagaatga atcgcagcca aggcattcat
     7021 caaaaaattg caataaaaag aaaagattac gtgcctgaat cttctcttta tcagcagtaa
     7081 actagtgggt attcatcccc ctacctcttc ccactaagag aatccttatg aaatccgttt
     7141 ttacgatttc cgccagcctg gcgattagcc tgatgctgtg ctgcacggcg caggcaaacg
     7201 accataaact cctcggcgtc attgcaatgc cgcgtaacga aaccaacgat ctggcgctga
     7261 aacttcctgt ttgtcgcatt gtgaaacgca tacaactctc tgccgaccat ggcgatttac
     7321 agttaagcgg tgcatcggtt tatttcaaag ccgcccgtag cgccagtcag agcctgaata
     7381 ttccttcaga aataaaagaa gggcaaacca ctgactggat caacattaac agcgataacg
     7441 acaataaacg ctgcgtctca aaaatcacct tttccggtca tacggtgaac tcatcggata
     7501 tggccacgct gaaaattatc ggcgacgact aacgccagaa tatagaagcc acaaaaaatg
     7561 aatgttaatt acctgaatga ttcagatctg gattttcttc agcattgtag tgaggaacag
     7621 ttggcaaatt tcgcccgatt gctcacccat aatgaaaaag gcaaaactcg cctctccagc
     7681 gtactgatgc gcaacgaact gtttaaatcg atggaagggc atcccgagca acatcgccgc
     7741 aactggcagc tgattgccgg agaattacag cattttggtg gcgatagtat cgccaacaaa
     7801 ctgcgcggac acggtaaatt gtatcgggcc attttgctcg atgtctcaaa gcgattgaag
     7861 ctgaaagccg acaaagagat gtctacgttt gaaattgagc aacagttact ggaacaattt
     7921 ctgcgtaata cctggaagaa aatggacgag gaacataagc aggagtttct gcacgcggtc
     7981 gatgccaggg tgaatgagct ggaagagctg ctgccgctgc tgatgaaaga caaattattg
     8041 gcaaaaggtg tgtcgcattt gctttccagc caactgaccc gcattttacg cacccacgca
     8101 gcaatgagcg tacttgggca tggtttgctg cacggcgcgg ggctgggagg ccctgtgggc
     8161 gcagcattaa atggggttaa agcggtcagc ggcagcgcct atcgcgtgac gattccagcc
     8221 gtactgcaaa tcgcctgcct gcgccggatg gttagcgcca ctcaggtctg ataccggaaa
     8281 ctggggaaaa ttttcatttt cccctcaaaa gatcgtagac actgccccac tggctgatta
     8341 ttatgccgcg ccctgaaaac actacagtta ttcagggaaa ttatttcacc attcattcga
     8401 tgatgatttt tgaggaatta tgggcaacac taagttggct aatccggcac cgctgggcct
     8461 gatgggcttc ggcatgacca ccattctgct taacctgcac aacgtgggtt atttcgctct
     8521 ggacggtatt attcttgcca tgggcatttt ctacggcggc atcgcgcaaa tttttgctgg
     8581 tctgctggag tacaaaaaag gcaacacttt cggtttaacc gcattcacct cttacggttc
     8641 tttctggctg acgctggttg cgattctgct gatgccgaaa ctgggtctga ccgatgcgcc
     8701 aaatgcacag ttccttggtg tctacctggg tctgtggggc gtatttacgc tgtttatgtt
     8761 cttcggcact ctgaaaggcg cacgcgtcct gcaattcgtt ttctttagcc tgaccgtgct
     8821 gtttgccctg ctggcgatcg gtaacattac cggtaatgcc gcgatcatcc actttgccgg
     8881 ctggattggt ctgatctgcg gtgccagcgc aatctatctg gcgatgggtg aagtgctgaa
     8941 cgagcagttt ggtcgcaccg ttctgccgat tggtgaatcc cactaattgt cttgagatcc
     9001 ccccgctcgg ggggattttt ttattcgcta acgtcgcgtc ttgcactctt cggtctgaat
     9061 gcagcaacca cttctggtgc cgtttcaacg tatggccctt ccagcaactg aatgcagtac
     9121 ggtacgctgg caaaaatacc atgcaccaca acgttaccct cagcgtcctt cacaccttcc
     9181 agcgtctctt taatagactt cggctgaccg ggtaagttaa ggatcagcgc ctgtttgcga
     9241 atcacgccca cctgacgcga aaggatcgca gttggtacaa aatgcaggct gatctggcgc
     9301 atctgttcac caaagccagg catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt
     9361 acgtcacgac gcgccgggcc agttccgccc gtggtgagca ccagatggca actcatttca
     9421 tccaccagct cacacaacgt ttgctcgatg atcgcctgct catcggggat taagcgggtt
     9481 tccagttcaa acggcgtggt tagcgccgat gccagccatt cttccagcgc tggaatgcct
     9541 ttatcctgat aaacgccgct ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa
     9601 gtattcatca agatttttcc gtttaaacag tctcgttaaa cagaatgata cactgcgaag
     9661 ggagtgacag acaggcttca gaagaggtag cgtgaccgac ttcccggtca cgctaagaat
     9721 gattacagca gatcgccgat cattttttcc agtttttcct ggtcaatagc aaacttacgg
     9781 ataccttccg ccagtttatc tactgccatt ggatcctggt tgtgctgcca caggaactcg
     9841 gactcagtga tacgcgccgg acgcgctttc acttcgccgg tgtaagacag tttacgttcg
     9901 atagcccctt cgctctccgc cagctctttc agcagtgccg gtgcgatggt cagacggtca
     9961 cagcctgcca gttccagaat ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt
    10021 tcataaccgt gctctttgta gtactggtag atttcagata cagaaaccac gcccggatct
    10081 tctgccggag cgtactcttt cttatcggta ttcgctttgt accagtcaag aatacggcca
    10141 acaaacggcg agatcaggaa cacgcccgcc tccgcacaag cacgagcctg agcgaaggag
    10201 aacagcaggg tcaggttaca gttgatacct tctttttcca gctgttctgc agcacggata
    10261 ccctgccagg tagaagccag tttgatcaga atacgatcgt tgctaatacc tgcatcgttg
    10321 tagagtttga tcaggcgttt tgctttcgca attgacgctt cggtgtcata ggaaagacgc
    10381 gcatcaactt cagttgagat acggcccgga accagtttca ggatttccag accaatattt
    10441 actgccagtt tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc
    10501 gcccaggcga cagcatcatc aatcaactta cggtattccg gaatctgcgc tgcgttaaga
    10561 atgagagaag ggttggttgt ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc
    10621 ccagtgtcgg ccactacggt ggtgtactga cgaagggagg tcaatttgtc cgtcatgata
    10681 gtatttctct ttaaacagct tgttaggggg atgtaaccgg tctgccctga tgatatcacg
    10741 acgcaacgtc agcgcaaccg ccgacaagac ggttatcgcc aggcgagact gtttcggatt
    10801 tctgacactg cgttgatatc gcccgccatt tttatacaaa acctcatgta tgctacgcag
    10861 aagttatcaa gtacctcgta gcgtatatac ttcttaaaca attggtaacg tttacacagg
    10921 aaagtcatcg cgaccggcaa taagagggat atgcatgcca gattttttct cctttattaa
    10981 cagcgtcctt tggggatcgg taatgattta cctgctcttc ggcgcaggtt gttggttcac
    11041 ttttcgcacc ggatttgtgc agtttcgcta catccgccag tttggcaaaa gtcttaaaaa
    11101 tagcattcat ccacagccag gcggtttaac ctcatttcag tcattgtgta ccagtcttgc
    11161 ggcgcgcgtg ggtagcggca acctggccgg cgttgcgctg gcaattaccg ccggtggacc
    11221 aggtgccgtc ttttggatgt gggttgctgc gtttatcggc atggcgacct cgtttgccga
    11281 atgttccctt gcacaacttt ataaagaacg tgacgtcaat gggcagtttc gtggcggacc
    11341 ggcatggtat atggcgcgcg ggctggggat gcgctggatg ggcgttctgt tcgccgtctt
    11401 tttgctcatc gcctacggca taattttcag cggagttcag gcgaacgccg ttgcccgagc
    11461 cctgagtttt tcttttgatt ttcctccgct ggtgacaggc attattctcg ctgtctttgc
    11521 tctgctggca atcactcgcg gtcttcatgg cgtcgcccgg ctcatgcagg ggtttgtccc
    11581 gttgatggcg ataatctggg tactgaccag cctggtgatt tgcgtaataa atatcgggca
    11641 acttccccac gtcatttggt ctatttttga gagtgctttt ggctggcagg aagcggcagg
    11701 cggcgcggcg ggatatacct taagccaggc gattactaac ggttttcagc gcagtatgtt
    11761 ttccaatgag gcgggaatgg gatcgacgcc aaacgcggca gcggcagctg cgtcctggcc
    11821 tccgcatccg gcagcacaag ggattgtcca gatgattggc atttttatcg acaccctggt
    11881 tatctgtacg gcaagcgcca tgctgatatt actggcgggt aacggcacaa cctacatgcc
    11941 gctggaaggt attcagctta tccagaaggc gatgcgggtg ctaatgggtt tctggggtgc
    12001 tgaatttgtt accctcgtgg ttattctgtt tgccttcagc tccatcgttg ccaactacat
    12061 ttatgccgaa aacaatctct tctttttacg cctgaacaac cctaaagcga tctggtgttt
    12121 gcggatctgc accttcgcaa cggtcatcgg cggcaccttg ctaagccttc cgctgatgtg
    12181 gcaactggca gatatcataa tggcctgcat ggctattacc aatttgaccg ccattttact
    12241 gctctcgcct gtggttcata ccattgccag tgattatcta cgccagcgta aactcggcgt
    12301 gcgcccggtg tttgatccgt tgcgttatcc ggatatcggt cgccagcttt ctccggacgc
    12361 gtgggatgat gtttcgcagg agtaatcaca actatcgatc aactcattct ctttttttgc
    12421 taaagtcggc ataaatttcc tgcaaggact ggatatgctg attcttattt cacctgcgaa
    12481 aacgcttgat taccaaagcc cgttgaccac cacgcgctat acgctgccgg agctgttaga
    12541 caattcccag cagttgatcc atgaagcgcg gaaactgacg cctccgcaga ttagcacgct
    12601 gatgcgcatc agcgacaaac tggcgggtat caacgccgct cgctttcatg actggcagcc
    12661 agatttcacg ccggcgaatg cccgccaggc gattctggcg tttaaaggtg atgtctacac
    12721 cggcttgcag gccgaaacct tcagcgaaga cgatttcgat tttgcccaac agcatttgcg
    12781 aatgctttcc ggcttgtatg gcgtactgcg cccgctcgat ttaatgcagc cttatcgtct
    12841 ggaaatgggg atccgtcttg agaatgcccg agggaaagat ctgtatcaat tctggggaga
    12901 tatcatcacc aacaagctga acgaggcgct cgcagcacaa ggcgataatg tggtgattaa
    12961 tctggcgtca gatgagtatt ttaaatcggt gaagccgaag aaattgaatg ccgagattat
    13021 caagccggtg ttcctcgatg agaagaacgg caaatttaag atcatcagct tctacgctaa
    13081 gaaagcacgc ggtctgatga gtcgtttcat tattgaaaac cggctgacca aaccagagca
    13141 actgactggt tttaatagcg aaggttactt ctttgatgaa gattcctcca gcaatggcga
    13201 actggtgttt aaacgctacg agcagcgtta agtttgtagt caatgccgga tgcggcgtga
    13261 acgccttatc cggcctacaa aagcatgcag attcaatata ttgcagaatt aacgtaggcc
    13321 tgataagacg cgccagcgtc gcatcaggcg ctgaatgccg gattgttacc cggcatttgt
    13381 catttagcgg tgatgtttgc ctggaccatg accgccgtga tgatcatgag gagctttctt
    13441 atggtggcgc ggcggtggcg gcggtccgtg tgggtgccag cgattgcctc gccattcata
    13501 atgttgtttc caccagccgt ggtcgcgcca gtgacctcca tcccagtaat agccacgatt
    13561 atcacgatcg cctatctgta attttactga cgggactaac gtaatttccg cagcctgtgc
    13621 tgccatggga gcgaccagaa ccagggaaag tgcgagtacg atagattgca tctttttcac
    13681 aggttactcc ttttatcatt gcccgttatg ggtcgatatc cggagattac gcgatgagaa
    13741 cgacggttgt tattctccgc aatcctaatc tctaagttac cgcatttatt gagaatttct
    13801 cctttttccc tgtcgttttt ctccataatt tcttcataaa aaagccgggc tgcataaaag
    13861 caaacccggc ctgattgaga taatgaatag attttactga tgattcatca tcaatttacg
    13921 caacgcagca aaatcggcgg gcagattatg tgaaagcaag ggtaaatcag cacgttctgc
    13981 cagctctttt ggcagatcca acgtttcacc gagaatcgct tccacgctct ctttaaattt
    14041 cgccggatgc gcggtgccga ggaacaagcc atattcgcct ggattcaact ggtcacgcag
    14101 cgcacgatac gctacggcag cgtgcggctc cgaggtgtag cccagttctt ttaactcacg
    14161 cattgtctgt tgcgtggttt catcatccac ggctgcataa cccagctctt tcagttgcca
    14221 gattttgcgg cggaacaact cttccacacg cggccagttg ttcggctgac tcacgtccat
    14281 cgcgttggat aacgtcgcct gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg
    14341 tggcacggta tcgttcacgt tggtcgcagc aataaaacgt ttcaccggca gaccgaatga
    14401 cttcgccagc agacccgccg tcaaatcgcc gaagtttccg cttggcaccg agacaaccag
    14461 ctggttgcgc gcctcctgcg gcagctgcgc aacagcttca aagtagtagc aaatctgcgc
    14521 cagcaaacgg ctgatgttaa tcgagttagc cgagtttaac cccagcgcca ctttcagttc
    14581 ttcatcatca aacgcctgct tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc
    14641 aacagtttcg atattgccgc ccaacgtaca gaacagtttt tcttgcagtg gactgatttt
    14701 gcctcgtgga tagaggataa ccactttcac attcggcaaa ccgtagaaag catgagccac
    14761 tgcagctccg gtatcaccgg aggtcgcggt cagaatggtc actggcttat catccgcaat
    14821 atgggtcagc atttgtgcca taaagcgacc gccgaaatct ttaaatgcca gcgttggccc
    14881 gtggaacaat tccagacaac cgacatcgct ttcaacattg gcgaccggag ccgggaaggc
    14941 aaacgccgcg cgcacgcgct cttccaggat ttcctgtggg atttcatcac caataaacgc
    15001 cgagaggatc ttcgcactgc gggtgacaaa atccagcttc agcatctcat caatttcagt
    15061 caggctgaat tccggcaggt cgtgcggaaa aaacagcccc tgatttttgc ccaacccctg
    15121 ggttacggct tgcgcaaagc tgacctgctc gttgtgatct ttcagattgt agagtttcat
    15181 ttagttttcc agtactcgtg cgcccgccgt atccagccgg caaatatgaa caaaaccttc
    15241 ctgattttgc aggtagttct tacccaacca gtcggcaacg cgctgggcgg tatccggctt
    15301 gtcacataga gcgaacaagg tcgggccgga gccggagata ccgctcgcta ccgcgccgat
    15361 ttccgcgacc gcctgccgcg cctgccggaa gccaggcagt aaccgttcac ggtagggttc
    15421 agcgataaca tctttcatca gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg
    15481 aatgaagcca gccagatgtc gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg
    15541 taaaatagcc cgggcttctg ccgtcgagac tttaattccc ggatacgcca gcacccacag
    15601 ccactcatca aaccctggca cttgctggct gatgatgtcg ttttcttcga tcatcaactg
    15661 cataccacca agaaaacacg gtgccacgtt gtcgtaatga atgctgccgg agatacgtcc
    15721 ttccagctcg cccatcaaag ccagcaaacg agtgtcatta agcggcttgc cgcagtgttc
    15781 attcatcgcc atcagcgccg cgaccaccga acaggcgctg gagcctaagc ccgaaccgat
    15841 cggcatattc ttttccagag tcatcgccac tggaatttgc ttgccaagct cctggcaaaa
    15901 acgctcccag cactgataaa cgatattttc ccgtggttct gacggcagct tatcggcaaa
    15961 gcgtccgagg ttgttgagac tgaatgtctc tgccgcctca accgtgacta catctccgag
    16021 caatgcacca tcaacgggtg ccaccgccgc cccgagcaca tcaaacccga cgctcatatt
    16081 ggcactggaa gccggggcat aaactttaac catgtcagac tcctaacttc catgagaggg
    16141 tacgtagcag atcggcaaag acaccggcag cggtaacgtc attgcccgca ccatatccgc
    16201 gcagcaccaa cggcagcggc tgataatagt ggctataaaa ggccagggcg ttttcgccat
    16261 ttttcacttt gaacagcgga tcattcccat ccacttcggc aatcttcacg cggcagacac
    16321 catcttcatc aatattgcca acatagcgca aaacttttcc ttcatcacgg gccttcgcca
    16381 cgcgcgcggc aaagagatcg tcgagctgtg acagattcgc cataaaagcg gcaacatcac
    16441 cctcggcgtt aaactctgcg ggcagcacag gttcaatttc aatatccgcc agctccagtt
    16501 cacgtcccgt ttcacgagcg agaatcaata gtttacgcgc cacatccata ccagaaagat
    16561 catctcgcgg gtccggttcg gtataaccca tttcccgcgc cagcgtggtc gcctcggaga
    16621 aactcatgcc ttcgtctaac ttgccgaaga tataagaaag cgaacctgaa agaatgccgg
    16681 agaacttcac caattcatca cctgcattga gcagattttg caggttctca ataaccggta
    16741 atccagcccc aacgttggtg tcatagagga atttacgccg cgatttttcc gccgcataac
    16801 gcaactgatg atagtaatcc atcgacgagg tgttggcctt tttgttcggc gtgacaacgt
    16861 ggaaaccttc gcgcaggaag tcggcatatt gatccgccac tgcctggctg gaggtgcagt
    16921 caacaatgac cgggttcagc agatgatatt ctttcacgag gcgaattaag cgcccgagat
    16981 taaacggctc tttggcttgt gcaagttctt cctgccagtt ttccagatta aggccatgca
    17041 cactagtgag cagagccttc gagttggcaa cgccgcagac acgtaagtcg atatgtttat
    17101 tcttcagcca gctttgctga cgcttcagtt gctccagcag cgcaccgcca acgccaccga
    17161 cgccaatcac aaacacttcg ataacctgat cggtattgaa cagcatctga tgagtaacgc
    17221 gcacgccagt ggtcgcatca tcgttattta ccacgacaga gattgagcgt tcagaagatc
    17281 cctgagcaat ggcgacaatg ttgatattgg cgcgggccag cgcggcaaag aatttcgccg
    17341 agatcccacg caaggtgcgc ataccatcac ctaccaccga gataatggcc agccgttccg
    17401 tcaccgccag cggctccagt aagccttctt tcagttccag gtagaactct tcctgcattg
    17461 cccgttcagc tcgcacacag tcgctttgtg gaacgcagaa actgatactg tattcggaag
    17521 atgattgcgt aatcagcacc acggaaatac gggcgcgtga cattgcagca aagacgcgcg
    17581 ccgccatgcc aaccatccct ttcatccccg ggccggaaac gctgaacatt gccatgttat
    17641 tcagattgga aatgcccttg accggtaatt cgtcttcatc acggctggca ccaatgagcg
    17701 tacctggtgc ttgaggattt ccggtatttt taatcaggca agggatctgg aactgggcga
    17761 tgggggtaat ggtgcggggg tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt
    17821 cctggtagga catcgacttc aacaacctcg catcgggcac ctgacgcggg tcgcaggtat
    17881 agaccccgtc aacgtccgtc caaatctcgc aacaatcggc gcgtaaacag gcagccagca
    17941 ccgccgcgga gtagtcggaa ccgttacgtc caagcaccac cagttcgcct ttttcattac
    18001 cggcggtgaa acctgccatc aacaccatgt gatcagccgg aatgcggctt gccgcaatac
    18061 ggcgggtgga ctcagcaata tcgacggtag attcgaggta atgccccact gccagcagtt
    18121 tttcgaccgg atcgataacg gtaacgttgt gaccacgcgc ttctaacacg ccggccataa
    18181 tggcgatcga cattttctcg ccacggcaaa tcagcgcagc gttgatgcta tccgggcact
    18241 gccccaacaa actaatgcca tccaggacat gttttatttg ggcaaattcc tgatcgacga
    18301 aagttttcaa ttgcgccagc gggaaccccg gctgggcggc ggcgagtccc gtcaaaagtt
    18361 cggcaaaaat acgttcggca tcgctgatat tgggtaaagc atcctggccg ctaatggttt
    18421 tttcaatcat cgccaccagg tggttggtga ttttggcggg ggcagagaag acggtggcca
    18481 cctgcccctg cctggcattg ctttccagaa tatcggcaac acgcagaaaa cgttctgcat
    18541 ttgccactga tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg ttacctttgg
    18601 tcgaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca
    18661 gcccgcaccg ttacctgtgg taatggtgat ggtggtggta atggtggtgc taatgcgttt
    18721 catggatgtt gtgtactctg taatttttat ctgtctgtgc gctatgccta tattggttaa
    18781 agtatttagt gacctaagtc aataaaattt taatttactc acggcaggta accagttcag
    18841 aagctgctat cagacactct ttttttaatc cacacagaga catattgccc gttgcagtca
    18901 gaatgaaaag ctgaaaaata cttactaagg cgttttttat ttggtgatat ttttttcaat
    18961 atcatgcagc aaacggtgca acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg
    19021 ctgtaaccag tcgaccagtt ttatgtcatc tgccactgcc agagtcgtca gcaatgccat
    19081 gactcgttcg cgtaaagctt gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg
    19141 ttgttgtatt aatgttgcta attgatagca atagaccatc accgcctgcc ccagattgag
    19201 cgaaggataa tccgccacca tcggcacacc agtaagcacg tcagccaacg ctaactcttc
    19261 gttagtcagc ccggaatctt cgcgaccaaa caccagcgcg gcatggctca tccatgaaga
    19321 tttttcctct aacagcggca ccagttctac tggcgtggcg tagtaatgat atttcgcccg
    19381 actacgcgca gtggtggcga cagtgaaatc gacatcgtgt aacgattcag ccagtgtcgg
    19441 gaaaacttta atattatcaa taatatcacc agatccatgt gcgacccagc gggtggctgg
    19501 ctccaggtgt gcctgactat cgacaatccg cagttcgcta aaacccatcg ttttcattgc
    19561 ccgcgccgct gccccaatat tttctgctct ggcgggtgcg accaggataa tcgttatacg
    19621 catattgcca ctcttcttga ccaaataacc gcgaacaggg tgatcactgt caacttatta
    19681 cgcggtgcga atttacaaat tcttaacgta agccgcagaa aaagcccttt acttagctta
    19741 aaaaaggcta aactattttc tgactgcact aacggtttaa ttgttaaaaa atgctacata
    19801 tccttctgtt tacttaggat aattttataa aaaataaatc tcgacaattg gattcaccac
    19861 gtttattagt tgtatgatgc aactagttgg attattaaaa ttatgtgacg aaagctagca
    19921 tttagatacg atgatttcat caaactgtta acgtgctaca attgaacttg atatatgtca
    19981 acgaagcgta gttttattgg gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat
    20041 ggttaggatg acagccgttt ttgacactgt cgggtcctga gggaaagtac ccacgaccaa
    20101 gctaatgatg ttgttgacgt tgatggaaag tgcatcaaga acgcaattac gtactttagt
    20161 catgttacgc cgatcatgtt aatttgcagc atgcatcagg caggtcaggg acttttgtac
    20221 ttcctgtttc gatttagttg gcaatttagg tagcaaacat gcagaccccg cacattctta
    20281 tcgttgaaga cgagttggta acacgcaaca cgttgaaaag tattttcgaa gcggaaggct
    20341 atgatgtttt cgaagcgaca gatggcgcgg aaatgcatca gatcctctct gaatatgaca
    20401 tcaacctggt gatcatggat atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg
    20461 aactgcgcga gcaggcgaat gttgcgttga tgttcctgac tggccgtgac aacgaagtcg
    20521 ataaaattct cggcctcgaa atcggtgcag atgactacat caccaaaccg ttcaacccgc
    20581 gtgaactgac gattcgtgca cgcaacctgc tgtcccgtac catgaatctg ggtactgtca
    20641 gcgaagaacg tcgtagcgtt gaaagctaca agttcaatgg ttgggaactg gacatcaaca
    20701 gccgttcgtt gatcggccct gatggcgagc agtacaagct gccgcgcagc gagttccgcg
    20761 ccatgcttca cttctgtgaa aacccaggca aaattcagtc tcgtgctgaa ctgctgaaga
    20821 aaatgaccgg ccgtgagctg aaaccgcacg accgtactgt agacgtgacg atccgccgta
    20881 ttcgtaaaca tttcgaatct acgccggata cgccggaaat catcgccacc attcacggtg
    20941 aaggttatcg cttctgcggt gatctggaag attaatcggc tttaccaccg tcaaaaaaac
    21001 ggcgcttttt agcgccgttt ttatttttca accttatttc cagatacgta attcatcgtc
    21061 cgttgtaact tctttactgg ctttcatttt cggcagtgaa aacgcatacc agtcgatatt
    21121 acgagtcaca aacatcatgc cggccagcgc caccaccagc acactggttc ccaacaacag
    21181 cgcgctatcg gcagagttga gcagtcccca catcactcca tccagcaaca acagcgcgag
    21241 ggtaaacaac atgctattgc gccagccttt caataccgcc tgcaaataaa taccgttcat
    21301 taacgcccca atcagactgg cgattatcca tgccacggta aaaccgatat gttcagaaag
    21361 cgccagcaag agcaaataaa acatcaccaa tgaaagcccc accagcaaat attgcattgg
    21421 gtgtaaacgt tgcgcggtga gcgtttcaaa aacaaagaac gccataaaag tcagtgcaat
    21481 cagcagaatg gcgtacttag tcgcccggtc cgttaattgg tattgatcgg ctggcgtcgt
    21541 cactgcgacg ctaaacgcgg ggaagttttc ccagccggta tcattgcctg aagcaaaacg
    21601 ctcaccgaga ttattagcaa accagctgct ttgccattgc gcctgaaaac ctgactcgct
    21661 agcttcccgt ttggctggta gaaaatcacc taaaaaactg ggatgcggcc agttgctggt
    21721 taaggtcatt tcgctattac gcccggcagg caccacagaa agatcgcctg taccgcttaa
    21781 attcagtgcc atattcagct gcaggttctg cttccgccag tccccttcgg gtaaagggat
    21841 atgcacgccc tgcccgcctt gctctaaccc ggtaccgggt tcaatggtca gtgccgttcc
    21901 gttaacttca ggcgctttca ccacaccaat accacgcgca tccccgacgc taatcacaat
    21961 aaagggcttg cctaagatga tatttggcgc gttgagctcg ctaagacgcg aaacatcgaa
    22021 atcggctttt agcgttaaat cactgtgcca gacctgaccg gtataaatcc ctatcttgcg
    22081 ttcttccacg ttctgattgc catcaaccat caatgactca ggtaaccaaa aatggataaa
    22141 acttcgtttc cgctccacgg ttttatcctc ttcctgcacc gtataaagct cggtcacagg
    22201 aatagcgata agcggcccaa cgagtttttg cggcccgctg gtactttggc gaatcgcatc
    22261 ttccacatcg ctacggtaat cggcacgttc gacaatcact tgccgaatca gcattatcgg
    22321 aatcaacaac agcaatactg caccaaacaa ggtggtcatt ttccagaaca ggggggattt
    22381 caacatagtc ttctcctttg caatggcagc aggatacgaa ggctatgtgg gaagaatttg
    22441 aagctatgtg aagtgacggt gaagtcgtag cgaggccagc acgccacctt cctgcacgtt
    22501 gcgcagcgtg acttcgccgt taaacaaacg ggcgacctca ctgacgaacg ccaaccccag
    22561 accgctgctt ttttgcccat ttgcacgcgg caaagagtaa aagcgttcaa aaatacgtga
    22621 cagcgcgtag tcaggaatac cactaccggt atccagcacc ttaagcgtga cgtgttcctg
    22681 atccacttcg gcgcttagcg ttatgcaacc gctctcggga gtaaaatcga tggcgttatc
    22741 cagtaaattc cccagcgcct gatccagtaa cgccggttca gcagcaacat taacctcgga
    22801 gggcataaca tgcaaagtga tgtttttttc tgccaactgc acggtgcgcg cttcgctgac
    22861 gcggcgaaat aatgccgcca catcaacagc agtcagaacg acttcctgac gattctccag
    22921 tcttgcctgg cgtagtaacg tttccaccag cgcctgcatt cgcgcatttt gtgtcagaat
    22981 gttgtcagta aaacgagcca ctacttcagg cggcggacct tcgcgtaaaa tttccgccgc
    23041 gccacgaatc gccgccagtg ggctttttag ctcatgggtt aacgcataaa catactgctc
    23101 aatatagttt ttcccttcca gcttcacgcg catactttcc agcgcctgcg cgagtttacg
    23161 cagctcgcta ctaccgagat cgggaagagg aacgggctta ttgtcagtga cggaatcagc
    23221 atagcgagtg agccgagcaa tggagcggtt gatccaccaa accatgcctg cgccaatcac
    23281 cagtgcaatc cccaacaaaa tggcgctggc ccataaaatt cgccgctcgc tgcgtttaat
    23341 gaccggagcc atcgccgcgt tcggtttgcc tacgctcaaa acgccaataa gccgcgagcc
    23401 gtccataatc ggcgcggcaa catacatcac cgaactttcg ggatcggcag gattttgcaa
    23461 cgtgctgcgc gcaccatact gaccacgcaa cgttagccag acgtcattcc agcgcgaata
    23521 atcctgtcca acggctttat ttaccgaatc gaacaatact ttgccatgcg catcggtcat
    23581 atagacatga tattcattgc gcactttgtt aatgccacca atattggcgc gaaacgggcg
    23641 atgttgtagc tgattaaacg cctgcgccag ttgcccatgc gttgggtccc cagagagcaa
    23701 atcgggacgc gccagctccg caagtaacgt tgccgtgtcg atcaacgtcc cctccgttgc
    23761 tcttcgcacg cccggtttaa cttctttgac aaaaatagcc agtacgaacc aggccgccac
    23821 cgccaccagt aaaaaatagc cccgcagcaa ccgcatgccg atacgcatta caagcccctc
    23881 aggctatatc ccatgccgcg atgagtatta atcggtgaaa gatcggggtt gatggcgcgc
    23941 agcttggcac gcagcgtttt aatgtggata tcgacggtgc gatcgtaggt gtcctgcgca
    24001 tcttcccaca cgctatccat tagttgctgg cgggaccaga cgcggcctgg cgacttgagt
    24061 aacgtcttca gcaataaaaa ctcataccgc gtcagcgtta atggcgtgtc aaaccagctg
    24121 atctgcgccg cgggttcatt caattcaaaa tgaccaatac ggatgacggg agacggcgtc
    24181 gagaacttct tcacccgacg cagtaaggtg cgcaccctgg cgcacacttc gcggggtgaa
    24241 aacggtttag ccacatagtc gtcagcgcca atttccagcc caagcaggcg atcgacctct
    24301 tcacttcggg ccgtcaggaa cagtacaggt aacgccggat ggagcgccag taactggcgg
    24361 cacaattcaa agccactgat atcaggcaaa ccaacgtcga gaatcatgac gtcgggaacc
    24421 tgctggcgag ctttatccag caccggcaaa cctcgctcaa agacctcgac ggcaaaacct
    24481 tcctgctgca acatgtagac cagcgtgtcg gctatccctt gctcatcttc cactaaccag
    24541 accgtttccc gttgcataaa tcgcctctgt tattgccgcc acggcatgac aggaaccgcg
    24601 ctaatcgcgt ttttcggcga accttctaca actttgtcgg agtaagccag ataagcgagc
    24661 gcgttgcgtt tggcgtcata aaagcgcacg acctgtaacg acttaaagac cagggacgtg
    24721 cgttttttga atactacctc gccctgagct ttgccgtttt taatacgatc cgacagttca
    24781 atcggcccga cttgctgaca agaaatggcc gcatcggagg tatcttctgc caggcccaat
    24841 ccccctttaa taccaccggt tttcgcccgg ctcacataac aggtgacatt tttcacatcg
    24901 ggatcgtcaa aggcttccac aacaattttg tgatccgggc cgatcatttt aaatacggtg
    24961 tcgaccgaac caatctcttc tgcatgagcc aatggcccca gcattatcag gcttaaagac
    25021 aggatcaaat gcttgtattt catattgtta ccatttttat ctattacacg agcaattagt
    25081 tcgtcacggt aaataacata ttgattaaga acaaaatctc aattctttta tttccgtcaa
    25141 gccctaaaac atactctact aagaaaaaaa cactgaatgc taaaacagca aaaaatgcta
    25201 ttatccaatt acctgatgtc aggtgctcgt tgttgaaagg atgaggatat tttatggatc
    25261 aggccggcat tattcgcgac cttttaatct ggctggaagg tcatctggat cagcccctgt
    25321 cgctcgacaa tgtagcggcg aaagcaggtt attccaagtg gcacttacag agaatgttta
    25381 aagatgtcac tggccatgct attggcgcgt atattcgtgc tcgtcgtttg tcgaaatcgg
    25441 cggtcgcact acgcctgact gcgcgtccga ttctggacat cgcgctgcaa taccgcttcg
    25501 actctcaaca gacatttacc cgcgcattca agaagcagtt tgcccagact cctgcacttt
    25561 accgccgttc tcctgaatgg agcgcctttg gtattcgccc gccgctacgt ctgggtgaat
    25621 tcacaatgcc agagcacaaa tttgtcaccc tggaagatac gccgctgatt ggtgttaccc
    25681 agagctactc ctgttcgctg gagcaaatct ctgatttccg ccatgaaatg cgttatcagt
    25741 tctggcacga ttttctcggc aacgcgccga ccattccgcc ggtgctctac ggcctgaatg
    25801 aaacgcgtcc gagtcaggat aaagacgacg aacaagaggt attctatacc accgcgttag
    25861 cccaggatca ggcagatggc tatgtactga cggggcatcc ggtgatgctg cagggcggcg
    25921 aatatgtgat gtttacctat gaaggtctgg gaaccggcgt gcaggagttt atcctgacgg
    25981 tatacggaac gtgcatgcca atgctcaatc tgacgcgccg taaaggtcag gatattgagc
    26041 gatactaccc ggcagaagac gccaaagcgg gagatcgccc aattaatctg cgctgtgaac
    26101 tgctgattcc tatccgccgt taacgctgca gctcatctaa tgcaggggcg tccagatgcg
    26161 agatgtcccc tgcggtttca acgacccagc cagacgccag ccacaggctt tcctgataat
    26221 ccacgcgcga aatagaacag ttacgcagac gtaagcggcg ctctgcccat gctggtaatc
    26281 cgagaatcgt actcaccaga catcccagtg caataccatg gctcaccagc agcggtcggc
    26341 ttccctgcgg taagtcccgg caagattcca gtgccgcatt gacgcgatcg ctgagctcct
    26401 gcattgactc gccttcagga atacgcccgt caacggtgcc attgaccagc tgccgacgcc
    26461 agttctcttc ttcttcggtc agagaatcga tatgtctttt ttccagcaca cccatgttta
    26521 attcacgcag gcgagaatca aagatgatgt cacagccgca ggcctgggcg atgatttccg
    26581 ccgtacgccg ggtgcgtcct aaatcgctac tgataatatg cgtaatgcca agctctttgg
    26641 cacgggttgc cacctgcatc gcctgttgct cacctttggc ggtcagcggg ctgtcagact
    26701 ggccctgaat acgtcgctcg gcgttccact gcgtttcacc gtggcggact aggtatacct
    26761 gtaacatgct ttttttccgt atactgctgt aacgttaatt ttctgagtaa tgctgattat
    26821 gcaccaagtt gtctgtgcga ccaccaatcc cgctaaaatt caggccattc tgcaggcatt
    26881 tcacgagatc ttcggcgaag gatcctgcca tattgcatcc gttgccgtcg agagcggtgt
    26941 accggaacaa ccctttggca gtgaggaaac gcgcgctggc gcacgaaatc gggtagcaaa
    27001 tgcccgccgt ttacttccag aggctgattt ttgggtggcg attgaagctg gcattgatgg
    27061 cgacagcact ttcagttggg tggtgattga aaacgccagc cagcgcggcg aggcgcgttc
    27121 ggcaactttg ccgttacctg cggtgattct ggaaaaagtt cgtgaaggcg aggcgcttgg
    27181 tcccgtgatg tcgcgttata ccggtattga tgagattggt cgtaaggaag gggcgatcgg
    27241 cgtgtttacc gccgggaaac tcactcgcgc cagcgtctat caccaggcgg tgatcctggc
    27301 gctgagtccg tttcacaatg ccgtgtatca ggcattgtag gacggataag gcgttcacgc
    27361 cgcatccgac attcggtgca cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac
    27421 aaaatcaatc gcttttcagc aacacctctt ccagccactg gcgcagctcc acgggcgcgg
    27481 ctttcaggct gttagatcca cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta
    27541 actcacgctg actcatttcg ccacgcaaca gctcttcgac aatacgcacg cgagtcccca
    27601 acgcttcgcg ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt
    27661 ggtaggcatt cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca
    27721 tcgctgctga atagggtgat tgttgggcca taatatgtcg ccattgttag cgggggaagc
    27781 aaaatgactc ccccggttgt actcgctaaa gagtacgata gcatatcata aacgtgcgga
    27841 tcagtaacga cgtccccatt ccgtggcgct catcaacgtc ggtttatccc ccatgaaata
    27901 gcggtagtaa gcatcataag ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg
    27961 aatactctcg acaaatgcca cagcgtcgat acgcccggcg ctgtggccaa gccaggttcg
    28021 cacccgtcca ggtccggcgt tataagctgc tgaggagaaa atacggttat tgccaaactg
    28081 ctgataaaca tattgcaggt aactggtgcc aatgttgatg ttcgtttccg gatccagcaa
    28141 ttgcccaggg ctgctataac caggaataga gaacatcttc accgtatggg tcgctgtacc
    28201 aggcataatc tgcatcaggc cgctggcccc taccggtgat ttcactttcg gattccaggc
    28261 gctctcctga cgggcaatcg ccatcgcata gctttgcggg atctctttcc cactggtgta
    28321 acgtttgaaa agatcgttgt aagccagcgg gaatcgctct tccagatgat cccaaagctt
    28381 cccggcgatc gttgcctgaa cgctaagatc ccaccattgg ttattgaaag cataccgagc
    28441 cagttgagcc tgctctgttt ttgacttgct cttcaccaga ttggcccact cgctacgcgc
    28501 ggtattatcg agattccagt acatcaactc gcgcacgcgc gccatctccg gcccctgagt
    28561 cagggtgctg tcaacattct gcggcgcttt atcaatcttc agctcatact cttcaccgat
    28621 gcgttgtgct gcaaccatcg ggtagaaacc acgctgttgc atgagttgat gcaaaatctc
    28681 tttcgcttca gcttcacgtc cgcgttccag caataaatcc gcctgccagt aacgccattc
    28741 atctttctct tttgcttcca tcggcagacg cgccagccag gtattcaggc cgcgacgatc
    28801 gccggtgcca agcgccattc gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat
    28861 aatggcgtca tcgcgccatt tcgcctgctc atcggtgaca tcattgccca tcaaacgcca
    28921 ggcgacgata tcgcgcagct cctgaatctg atcttcatta agctgctgcg cctgggcaag
    28981 cgacgggatc atcagccgtg cattttcggc atcctgccgc gccacactgg caaacgccac
    29041 cgccgccatt tgacgggtaa aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt
    29101 attagggttg ttcgccagtg aaatgattgc cgaggcgata gtctggtaat cggcaggcat
    29161 ctgccctgcc agcactgtta ctaggcctgt gttacccgct ttcatcgcca gacggatacg
    29221 ctctaaatac gccagcggat cttgtttacc tgacgcacgc cagacgctaa ataacttgtc
    29281 acaggcgtta ggctggctct tgccggttag ccacagctct ttcgcccctt gccaggcttc
    29341 ttcactctgc ccggtgttcc atttcgcata gtagtaatta cattgcgctt cggtagttcc
    29401 gggcttttcc gggctaaagg ctaacaagcc acgccagtct tcacgccgcg ccagttcatt
    29461 gacgaaacga gattgcagcg tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt
    29521 agtgaccgtc accgccggtt gattcatcag gtcatcggtg atctggcggt attccaggta
    29581 gggataaagc ggataatcct tcagtccagg catcatttgt tcgaccacat ccatctgtcg
    29641 attatcccag gcctgcttga tttgcgcgta acggctacgc tgctcatcca gtgagtcggc
    29701 tcgcgccacg ctgctgaccg tcagcagaca gacaccggca gccaacagcc gccaggtaac
    29761 ttgtttggct ttttccacaa gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg
    29821 ccgcgtaata gatttatgct aaccagtcat tgccgtttac gccacgttac ggacactttt
    29881 ttacttttac tgcgaaggtg atcgaccaac tgggattagg cggggaaaca ggctacactt
    29941 cgcctttgaa aactcatcat cacgataaac atagaggcga agtccaacgt ggctcaattc
    30001 gtttatacca tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat tttgaaaaac
    30061 atctctctga gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt
    30121 aagtccaccc tgctgcgcat tatggcgggc attgataaag acatcgaagg tgaagcgcgt
    30181 ccgcagccag acatcaagat tggttatctg ccgcaggaac cgcagctgaa cccggaacac
    30241 accgtgcgtg agtccattga agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg
    30301 gatgaagtgt atgcgctgta cgccgatccg gatgccgatt ttgacaagct ggccgctgaa
    30361 caaggccgtc tggaagagat cattcaggct cacgacggtc ataacctgaa cgtacagctg
    30421 gagcgtgcgg cggatgcgct gcgtctgcca gattgggacg cgaaaatcgc taacctctcc
    30481 ggtggtgagc gtcgtcgcgt ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg
    30541 ctgctcgacg aaccgaccaa ccacctggat gccgaatccg tggcgtggct ggaacgcttc
    30601 ctgcacgact tcgaaggcac cgtggtggcg attactcacg accgttactt cctcgataac
    30661 gttgcaggct ggatcctcga actggaccgc ggtgaaggta ttccgtggga aggtaactac
    30721 tcctcctggc tggagcagaa agatcagcgt ctggcgcagg aagcttcaca agaagcggcg
    30781 cgtcgtaagt cgatcgagaa agagctggag tgggtacgtc agggaactaa aggccgccag
    30841 tcgaaaggta aagcacgtct ggcacgcttt gaagagctga acagcaccga ataccagaaa
    30901 cgtaacgaaa ccaacgaact gtttattcca cctggaccgc gtctgggcga taaagtgctg
    30961 gaagtcagca acctgcgtaa atcctacggc gatcgcctgc tgattgatga cctgagcttc
    31021 tcgatcccga aaggggcgat cgtcgggatc atcggtccga acggcgcggg taaatcgacc
    31081 ctgttccgta tgatctccgg tcaggaacag ccggacagcg gcaccatcac tttgggtgaa
    31141 acggtgaaac tggcatcggt tgatcagttc cgtgactcaa tggataacag caaaaccgtt
    31201 tgggaagaag tttccggcgg gctggatatt atgaagatcg gcaacaccga gatgccaagc
    31261 cgcgcctacg ttggccgctt taactttaaa ggggttgatc agggtaaacg tgttggcgaa
    31321 ctttccggtg gtgagcgcgg tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac
    31381 atgctgctgc tcgacgaacc aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa
    31441 aacgccctgc tggagttccc gggctgtgcg atggttatct cgcacgaccg ttggttcctc
    31501 gaccgtatcg ccacgcacat cctggactac caggatgaag gtaaagttga gttcttcgaa
    31561 ggtaacttta ctgagtacga agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag
    31621 ccgaagcgta tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc
    31681 gaacgcctta tccggcctac aaaaccagca atttcaatac gttgtaaaac tgtaggcctg
    31741 ataagacgcg gtaagcgtcg catcaggcat tgagcaccga atgccggatg cggcgtgaac
    31801 accttttccg gtctacaaca gccttagcat cctcaatttc gtggcgggtc acccgtgcag
    31861 gttttttcaa accttgttta atctcgacag cctcttccag agaggttttc aactcatcaa
    31921 aaaaactcat tttatacctc gcctttgagc agtttcgtca cggttatcct tgctccccca
    31981 tcatctcccg caccagctcc acgcagcgca ggaaacggct gtcgtaatcg tcctcttcaa
    32041 cccgcacgaa ttcgatattg ttctcttcca gcatctccac cagcaagttc tggaactctt
    32101 tgcgatccac cgaactgccg aggctgcgta aaccatccgc cacccacggc gtgttgttct
    32161 ccagcaggat caccagatcg aaacggtatt catcaatcag cgcctgcacg aacggatgct
    32221 cacgcccttc gtactttttg cagaacgcct gggtagtgac aaaatcggtg tcaataaacg
    32281 ccactttatt ggcgtatttc actgcaaaat caatgtattg tgcgtggccc aacgcgattt
    32341 tatcgtagtc ggaatactgc aatgcgatct catcaccgcc gaggtgtgaa aagacataat
    32401 cgcggccata ttcccacgca ctggtggtgt tgaagatatt ggcaagtttg tttaccaggg
    32461 tggatttacc gctcgactcg ccgccaagga tcgccacggt acgcacaaaa aacggcttca
    32521 cttcggtagg aatatattcc cagtagcgga acgggttttc gcggatctgc gcaccgctga
    32581 tactcataaa ggtacgtttc ggatcgacca gcaccgtctc gatccccaga tgttccatat
    32641 actgtggcgc atcggcttct tccgaggtgt agatcaggtc cggctggatc cccttttcag
    32701 ccataaactt tttgatgccg ttgctccaca catcccagcc atgcggatac ggctccatgc
    32761 cctcttcgtt gaaagcatga atacggatat ttttctgata tttaaaggtt tgcaatagcc
    32821 agcgcagacg atccggcacc gtgggctgct gcgacatggc gctgtcttca aacaacgcgc
    32881 ggtcgcgggt atcgtcaaaa cccataatga tatgcagctc gtcaacctgg ctacaggcgc
    32941 gctggataag gtagatatgt ccggtatgca gcgggtagaa cttaccgaat acgacaccaa
    33001 tggttttctt ctgccgggga aattcaagcc ccaaaaaacg gtgcaatgcc tccagctttt
    33061 gcgcgctggg gcttttaatt ttggcattca gtaactggct taaataccct ttggtcatac
    33121 cgctggcatc agctacctgc tgtagcgtgc agccctgttg cttgatggca gttttcaggt
    33181 aatcaaatga cgacatatct ccctccgtat ctctcattat aagtcgtcga acacgctaag
    33241 cgcgtcggag agttttttaa cgccaaaaat ctgcatccct tccggcgctt ttttcggtac
    33301 gttagccgcc ggaacaatcg cccggcgaaa accgtgtttc gccgcttcag aaatccgctc
    33361 ctggccactg ggcaccgggc ggatctcccc tgccagcccg acttcaccaa acaccaccag
    33421 atcctgtggc agcggtctgt cacgcaggct ggaaaccatc gccagcagta acgctaagtc
    33481 ggcgctggtt tcggttacct tcacgccgcc gaccacgttc acaaacacat cctgatcggc
    33541 catttgcaga ccaccgtgac ggtgcaagac agccagcagg attgccagac ggttttgttc
    33601 cagccccact gccacgcggc gcggattcgc cattatcgag tgatcgacca gcgcctgaat
    33661 ctccaccagc aacggacgcg ttccttccca caccaccatc acggagctgc cggaggtcac
    33721 ttcatctccg cgacttaaga aaattgccga agggttgctg acttcacgca gcccctgttc
    33781 ggtcatcgcg aagacgccca gctcattcac cgcgccgaag cggtttttat ggctgcgtaa
    33841 ggtgcgaaaa cgggagtcgg catcgccatc cagaagcacc gaacagtcga tacagtgttc
    33901 cagcactttc gggccagcca gcgaaccatc tttggttacg tgtccaacca tgacaatcgc
    33961 cacaccgcgc gttttggcga aacgcgtcag ataagccgcc gtttcacgca cctgcgccac
    34021 gctaccaggc gatgattgta catccgccat atgcatcacc tggatcgagt caattaccat
    34081 cagcttcggt tgctcttctt cggcaatcag gcagatctgc tcgatgctgg tttccgacaa
    34141 catattgaga ttgtcagtcg gcaggccaag gcgatgagcg cgcattgcca cctgttgcag
    34201 cgactcttcg ccggtgacat acagcgtttt catctgctgg gccagtttgc acagcgtttg
    34261 cagcaacagc gtggatttcc ccgcaccagg gttaccgcca atcagaatgg cacttcctgg
    34321 caccacgccg ccgcctagta cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag
    34381 ctcttcaagg ctgatatcgg agagtttctg gacttttgcc accccggcgc taccggcata
    34441 gccgctgaga cgctcgttac gcgccaccgt tggcgacgca gcaagacgca cctcggtgat
    34501 ggtgttccag gcatgacagg cactgcactg cccctgccag cgcggataat cggccccgca
    34561 ttcattacaa acaaaggcgc gttttggagc ttttgccacg gtgtacctcg ttaatgctgt
    34621 gccgcccgca ggatgacggg caggcaatta cttctgattc aggctgccag agaggatgca
    34681 gaataccccc atcaggtcag cgtgacggat ggtgacttcc gccttttcat tcacttttgg
    34741 cttggcatgg taggcaatcc ccagccctgc tgctttgatc atctgcaggt cattagctcc
    34801 atcgccaatc gccacggtct gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag
    34861 agttttcgct ttgtactgcg cgtctacgat gtcgccgatc acattgccgg taaatttacc
    34921 gtccatgatc tccagttcat tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta
    34981 ttcagcaaag aaagtaaagc cgccggaggc aatcgccact ttccagccca gcgtttccag
    35041 cttgagcacc agctgcgtta agcctggcat cagcggcaga ttttcacgca cctgttgcag
    35101 aatattggcg tcagcgcctt tcagcgtcgc cacgcggctg cgcaggctgg cggtaaaatc
    35161 gagttcgccg cgcatcgccc gttcggttac ctccgccacc atctcgcccg ttccggccag
    35221 cttggcaatt tcatcaatac attcaatctg gatggcggtg gaatccatgt ccatcaccag
    35281 caaacccggc gtgcgcaggt gcgggatttt ccccagcggg gcgacatcca gctgcgcttc
    35341 gtgggccagg cgtgtagccc gtgcggtgag tgaacctgcc agacgaatca cctgataatc
    35401 ttccacgcac caggcggcaa caatcaccat cgccgcaccc agtttgctct ggtattgggt
    35461 cagacgctgt ttatccagcc cacgaccata cagcagccag ccgctacgac ctgcgtggta
    35521 atccagtggc atcacttcat caccacttaa tgaaagaggc agacccggcc ataaagagac
    35581 atcttcaggc aggtcgcacc aggtaatgtt aggcattaag gctccagtaa aatcgttcga
    35641 agcagggaaa ataacgcatg aggctacctt gtatccattg cttctggcaa cattaagtct
    35701 caaattttca aagggtggaa gatggctcgc acaaaactga aattccggct acatcgggca
    35761 gtgattgtcc tgttctgtct tgccttgtta gtggcactga tgcagggggc gtcatggttt
    35821 agtcaaaacc accagcgaca gcgtaatcca cagctggaag aactggcccg caccctggcg
    35881 cgtcaggtga cgctgaacgt tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc
    35941 attcaggcga tcctcgatca gttaacggat gaaagccgta tcctcgacgc gggtgtgtat
    36001 gacgaacaag gcgatcttat cgcacgttct ggcgaaagcg tcgaagtgcg cgatcggctg
    36061 gcgctcgacg gtaaaaaagc aggcggctat tttaaccagc agattgtcga gccaattgcg
    36121 ggtaaaaacg gaccgctcgg ctatctgcgc ctgacactcg acacccatac gctcgccacc
    36181 gaagcccaac aggtggataa caccactaac attttacgcc tgatgttgct gctctcactg
    36241 gcaatcggcg tagtgctgac ccgcacgctg ctacagggta aacgcacccg ctggcagcaa
    36301 tcgcccttcc tgttaaccgc cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa
    36361 gagtgaccca ttactacaag aaaggaaatc gttatgtcca cattacgcct gctcatctct
    36421 gactcttacg acccgtggtt taacctggcg gtggaagagt gtatttttcg ccaaatgccc
    36481 gccacgcagc gcgttctgtt tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg
    36541 cagaacccgt ggaaagagtg taatacccgg cggatggaag aagataacgt ccgcctggcg
    36601 cggcgcagta gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg ctttaccttt
    36661 atggctggca agccggagta cgataaaact atctccacgt cgattgtgct caatgcgctg
    36721 aacgcgctcg gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc
    36781 gaaggcgacc gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac
    36841 cacggcacct tgctactcaa tgccgacctc agccgcctgg caaactatct caatccggat
    36901 aaaaagaaac tggcggcgaa aggcattact tcagtgcgtt tccgcgtgac caacctcacc
    36961 gagctgctgc cggggctcac ccatgagcag gtttgcgagg ccataaccga ggcctttttc
    37021 gcccattatg gcgagcgcgt ggaagcggaa atcatctccc cggacaaaac gccagacttg
    37081 ccaaacttcg ccgaaacctt tgcccgtcag agtagctgag agtggaactt cggtcaggct
    37141 ccggcattct cgcatctgct ggatgaacgc tttagctggg gcggcgtgga actgcatttc
    37201 gacgttgaaa aaggccatat cacccgcgcc caggtattta ccgacagcct caaccccgcg
    37261 ccgctggaag ccctcgccgg gcgactgcaa ggctgcctgt accgcgcgga tatgctgcaa
    37321 caagagtgcg aagcgctgtt ggttgacttc ccggaccagg aaaaagagct acgggagttg
    37381 tcgacgtgga tagcgggggc ggtaaggtaa ctcgctgatt aaaatatcca aggaaggaaa
    37441 tatgatcgat ctggaaaacc aggaacggga gattatcaat ttaatgttca gccagcgtat
    37501 ttcctggcta gccgccgtgc gtatcaggca taaattatcg cttgctgaag taagtaaaat
    37561 gttaggtatc tcgattaatt ctctaaaaca gatcgaaaag acggaacggc ttagctcaaa
    37621 catcaaaagc aaaatggcag aaatttatgg ttgtccacca gagttgctga tatgcccttc
    37681 ctggatgacg gctgaacata aatgaataga ttcgtgaata gatacttgaa tgtattttat
    37741 tttctatcca tatgaaaaat aatagtaaat ctataaatca gttgctattg atgaatagat
    37801 actaaaaagc cggagcagtc ccccactccg gcttaacacg acaatcgcct ttcagcgaaa
    37861 cacaattact ctttatcgcc cagcagaacg gattccagtg cgattttgat catgtcgttg
    37921 aaggtggtct gacgctcagc ggcagtggtc tgctcgtgag tgcggatgtg gtcagacacg
    37981 gtgcagatgg tcagggcttt cgcgccaaac tctgcagcca cgccgtagat accagccgct
    38041 tccatttcca cgccgaggat gccgtatttt tccatcacgt cgaacatttc gccgtccgga
    38101 gagtagaaca ggtcagcgga gaacaggtta cccacgcgag catcaacgcc cagtgcttta
    38161 gctgcatcta ctgcgttacg caccatgtca aagtcagcga tagcggcaaa gtcatggtct
    38221 ttaaaacgga tgcggttaac tttggaatcg gtgcaggccc ccataccgat aacgacgtcg
    38281 cgcagtttta cgtgcggcag aactgcgcca caggaaccaa cgcggataat tttcttcacg
    38341 ccgaaatcgg tgatcagttc tttggtgtag atggagcagg acgggatacc cataccgtga
    38401 cccattacgg aaattttgcg gcctttgtaa gtaccagtga agcccagcat accgcgaacg
    38461 ttgttcactt cacgagcatc ttcaaggaaa gtttcagcaa tatacttcgc acgcagcggg
    38521 tcgcctggca tcaaaactac gtcagcgaaa tcgcccattt ctgcattaat gtgtggggta
    38581 gccattgttt tatccttttt gtgacataac aaaggcggag tcaacgctcc gcccaaatcc
    38641 atcagaacat ggctttgcca tattccatat cagaagtacc aaaatatttt gccagagtct
    38701 ggccgatatc cgcgaaggtt tcacggtgac ccagtgagcc cggttttact ttcgggccat
    38761 acaccagtac cggaatgtgt tcacgcgtgt ggtcagtacc ggtccaggtc ggatcgcaac
    38821 cgtggtcagc ggtgaggatc aggatgtcgt catcgcgcag cagagacatc agctccggca
    38881 gacggcggtc gaacagttcc agacccgcag cataaccggc gacgtcgcga cggtggcccc
    38941 aggaagagtc gaagtcaacg aagttggtga agacgatggt gttatcaccc gcttctttca
    39001 tctctttgat ggtggcgtca aacagcgcgt ccaagccagt cgctttcact tttttggtga
    39061 taccgcagtt ggcgtagatg tctgcaattt taccgacaga aaccacctgg ccgtgttttt
    39121 catcaaccag tttctgcagc acggtcggtg ctggcggctc aacagccagg tcgtgacggt
    39181 taccggtacg ctggaagtta ccagctttgt caccgataaa cggacgagcg ataacacgac
    39241 cgatattgta gccgccgttg gtcagctctt cacgggcgat ttcgcacagt tcgtagagtt
    39301 tatccagacc gaaagtttct tcatggcagg caatctggaa cacggagtca gcggaggtat
    39361 agaaaatcgg cttgccggtt ttcatgtgct cttcgcccag ttgatccaga atgaccgtac
    39421 cggaagagtg gcagttaccg aggtaacccg gcagattagc gcgttcgacc agtttatcca
    39481 gcagctcttg cgggaagctg ttttcgtgat cggagaaata tccccactca aacagaaccg
    39541 ggacaccggc gatttcccag tgaccagacg gggtatcttt accggatgac atttcgtgcg
    39601 cccatgcgta cgcgccgata acttcagcgt tgccgtccat tcccgccgga atgaaaccgg
    39661 tagaaccttc gtgtgctttc gccagcccca gacgggtcag atttggcaga ttgagcggcc
    39721 ctttacgacc attatcagct tcgcctttgg cacaagcttc tgcgatatga cccagggtgt
    39781 cagccccgac gtcaccaaag cgttctgcat cttctgtagc gccgatgccg aatgagtcca
    39841 gcaccataat aaatgcacgt ttcatatgtt ctccgtactc agtgcttcaa atttaaatga
    39901 tcagatcagt ataccgttat tcgctgatac ggcgatagac agttggtgtg ctttccggtg
    39961 ctttatcggc aagtttaatt gccgctttca ccgctttcgc cgcttcctgc cagctgtttt
    40021 cgtctttcgc taggataacc gccagcggac gctgaccgtc tacctggtcg cccagacgcg
    40081 ccatatcagt aaagccgacg ctgtaatcga tggtgtcaga tgcctgacga cgaccgccgc
    40141 ccattgcaac cactgccatc cccagcgcgc gggtatccat ttcactgaca aaaccttcgg
    40201 tatcagcata gactgctttc gtcagcatcg ctgtcggcag atacttcgcg tagttctcaa
    40261 cgaagtcggt cgggcctttt tgtgccgcta ccatacgacc aaagacttct gccgctttac
    40321 cgttgtccag caccgcctgc aatttcgcgc gcgcttcggc gtcatctttc gccagtttgc
    40381 cggagatcag catctccacg cacagcgcca tcgtgacatc aaacagacgc gggttacgat
    40441 actcacccgt caggaactgc accgcttcac gaacttcaac cgcgttacct gcactggagg
    40501 ccagtacctg attcatgtcg gtgagcagcg cggtggtgcg cacgccagcg ccgttagcca
    40561 cgccaacaat cgcttcggca agggcttcag agagttcgta ggtcggcata aacgcgccgc
    40621 tacccacttt cacgtccatc accagcgcgt ccagaccttc cgccagtttc ttcgccagga
    40681 tcgaggcagt gatcagcggg atggagtcca cggttgcggt aatatcacgg gtcgcgtaga
    40741 aacgtttatc agccggagcc agtgaactgg tctgaccgat aatcgccacg ccgacgtctt
    40801 taataatttc gcggaaacgg ttgtcatccg ggaaaatgtc gaagccaggg atggattcca
    40861 gtttgtcgag cgtaccgcca gtatgaccga ggccgcgacc ggagatcatc ggaatatagc
    40921 cgccgcaggc tgcgaccatc ggccccaaca tcagcgaagt gacatcgccg acgccgccgg
    40981 tcgagtgttt atcaacaatc gggccattca gatgcaggct tttccagtcg agaacggttc
    41041 ctgaatctcg catcgccatg gtcagcgaga cacgctcagg cattgtcata tcgtggaaga
    41101 aaatggtcat cgcgagggcg gcaatctgcc cttcggaaat agtgttgtcg cgaataccgt
    41161 tgataaagaa acgaatttct tcatcgctca gcgcatgacc atcacgtttt ttacgaataa
    41221 tttcttgtgc gagaaacaag gtaacctccc gaggaaaaga gtatgaggcg gcttttgccg
    41281 gatgcgtcta cgccttatcc ggcctacggg tagatacgtc tcgtaggcct gataagcgca
    41341 gcgcatcagg cgtaaagcat cttacttagt agctgctggc gctcttaccg tcgccgtggc
    41401 ccagcgcttt cagcaggctt gccagcaggc tggaagcacc aaagcggtag tgacgcgcat
    41461 cagcccagtc agcaccgaac agttcatctg cgatggcgag atatttctgc gcatcttccg
    41521 cagtacgcac gccgcccgcc ggtttgaaac caacggtttt ttctacgccc atatcacgga
    41581 tcacttccat catgatgcga gcgctttccg gcgtcgcgtt cacagccact ttaccggtag
    41641 aggttttgat gaagtccgca cccgctttga tggagatttc agacgcttta cggatcagcg
    41701 cttcgtcttt cagttcgccg gtttcgatga tcactttcag cagtacattc gctgccgcgc
    41761 aagcctcttt acaggctttc actaggtcaa aaccaacctg ctcgttaccc gccatcagcg
    41821 cgcggtacgg gaacaccacg tcaacttcat cagcaccgta ggcgattgcc gcacgggttt
    41881 ctgccagcgc gatttcgatg tcgtcgttac cgtgtgggaa gttggttacc gtagcgatac
    41941 ggatttccgg ggtgccctgc tctttcagag ttttgcgagc aatcgggata aaacgaggat
    42001 agatacagat agcggcggta ttgccgaccg gagttttggc ctgatgacac agagcaatta
    42061 ctttctcgtc ggtgtcgtcg tcattcaggg tggtcaggtc catcaatttc agtgcacgca
    42121 ggctgcttgc tttcagatca gtcatttcat tctcctggct tgtcgccaat aaaattcacc
    42181 ttgcgagttt gttagtattc taacatctac tccgcaacac acttcgatac acatcacaat
    42241 taaggaaatc tacttacaag tttgcatcac tgtaatgcga tctggttcaa ataattcact
    42301 ttcaaatgaa tgcgtcagtg gtggcaaacg catcaggatc aaagtgaaca tcacgaaact
    42361 tcttacaatg gcgcatcacc ttcggcataa aaggaaaaga agatgcccac ttctcatgaa
    42421 aatgcactgc aacaacgttg ccagcaaatt gtcaccagcc cagtgcttag cccggagcag
    42481 aagcgccatt ttctggcact ggaagcagaa aacaatctgc cttacccaca gcttcctgcc
    42541 gaagcccgcc gcgcgctgga tgaaggtgta atctgcgata tgtttgaagg tcatgcgccg
    42601 tacaaaccgc gctatgtctt acccgattac gctcgttttc tggcgaacgg ttccgaatgg
    42661 ctggagctgg aaggcgcgaa agatcttgat gacgcactct ctctgctgac cattctttac
    42721 caccacgtac cgtcggtcac atcgatgccg gtctacctgg ggcaactgga tgcgttgttg
    42781 caaccgtatg ttagaattct aacacaaaac gagatcgatg ttcgaataaa acgtttctgg
    42841 cgttacctcg acagaaccct gccagatgcc tttatgcacg ccaatatcgg cccgtctgat
    42901 tcgcccatta cccgcgcgat cttacgtgca gacgcgcagg tttcaccgaa cctgaccttt
    42961 atctacgatc ctgaaatcac ccctgatgac ctgctgctgg aagtggcgaa gaacatctgt
    43021 gaatgtagca aaccgcacat cgccaacggt ccggtgcatg ataaaatttt cacaaaaggg
    43081 ggctacggga ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg cagcacgctg
    43141 gtacgcctta acctgaaagc cattgccgag cgcagcgaat cgctggatga cttctttacg
    43201 cgcactctac cgcactactg ccagcagcag atcgccatca tcgatgcgcg gtgtgaattc
    43261 ctctatcaac aatcacactt ctttgagaat agcttcctgg tgaaagaagg gctgattaac
    43321 cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg cggaagcggt taacttgctg
    43381 tgtgagaaag aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa tgaagtgggt
    43441 tatcgcatca gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa atatggctgg
    43501 caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt ccgatatcgg caccacgccg
    43561 ggcgcgcgtt tgccgtatgg cgatgagcca gatccgatca cccatctgca aactgtcgcc
    43621 ccgcatcatg cttattatta ttccggcatc agcgacattc tgacgctcga cgaaatcatc
    43681 aaacgtaacc cgcaggcact ggtacagctt tgcctcggtg cctttaaagc cggaatgcgt
    43741 gaatttaccg ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta tatggtgcgt
    43801 ttgtcggatt tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac ctggctgggc
    43861 gaagaagccg cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat aagccatgaa
    43921 cagcagatgc gctttagtca gtaagattat ccccttctcc tgcgttgacg ggccaggcag
    43981 tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc acaatccgtg
    44041 gacgatggga cgttgcaatg gctgtgggga gtgcgtgcca cagtgtccgc atcaggcgtt
    44101 gcagattgtt gacggcaaag tggtgtggaa cgctgtggtc tgcgagcaat gtgatacctg
    44161 cctgaagatg tgtccgcaac atgccacacc aatggcgcaa tccatgagcg tggacgaagt
    44221 gcttagccat gtgcgcaaag cggtgctgtt tattgaaggg ataacggtaa gcggcggcga
    44281 agccacgacc caactgccgt ttgtagtggc gctgtttact gctatcaaaa acgatccgca
    44341 actgcgccat ctcacctgtc tggtggacag taacggtatg ttgagcgaaa ccggctggga
    44401 aaaattactc ccggtgtgtg acggcgcaat gctcgatctc aaagcatggg gtagcgaatg
    44461 tcatcaacaa ttaaccggac gcgataatca acagattaag cgcagcatct atttgctggc
    44521 agagcgcggc aagctggcgg aactgcgttt gctgatgatc ccaggccagg tggattattt
    44581 gcaacacatc gaagaactgg cggcgtttat caagggactt ggcgatgttc cggttcgcct
    44641 gaacgcgttt catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga gcgccacgcc
    44701 ggaagacgtt gagccgttgg ctgatgcatt aaaggtgcgc ggggtgagcc ggttgatatt
    44761 tccggcgctc tatttgtgac atgccggata cggcctacgg ttcggcacag acttgtaggc
    44821 ctgataagac gcgtcaagcg tcgcatcagg cattgtgcgc caactgccgg atgcggcgtg
    44881 aacgccttat ccggcctacg gttcggcaca aacctgtagg cctgataaga cgcgttaagc
    44941 gtcgcatcag gcattatgcg ccgactgccg gatgctgcct acgaagggca aactacccac
    45001 gaacgttaaa caccgcatac gtgttattaa gcaacacttc ggcaatctca tccgcgggtt
    45061 ccgggcgtaa ctcgcaaagc acggcaaaca cacgggcagc ctgttccggg cggttaggct
    45121 gcccctgaaa accgttgagc ggcatatccg gcgcatcggt ttccagcaac aacgatgcca
    45181 gcggtaattt tgcgatgaca tcgcgggttt tactggcgcg tggataggtg atagtaccgc
    45241 ctacgccgat tttgtagccc agctgtacaa accgttcagc ctgttgctgg ctgccagaaa
    45301 atccgtggac aacaccagca cgcggcaaat cgtggcgttt aagatgcatc gccagtttgt
    45361 cgtgcgtgcg ccgtgaatgc aggatcaccg gcagatcgta gcgtttcgcc agtttcagtt
    45421 gttcgtcgag taaccactgc tgcctctcaa attgcggatc gtcgccgaag agatccagac
    45481 cgatctcccc caccgccacc accttcgccg gacgcctttc cagcgcctgc tgtagctgct
    45541 caagagacac atcgctatgt ttttccaaca taccaggatg caagcccaat gcggcataca
    45601 gcggttgata attttccgct aacgcctgca cgcgggcaaa attttccgcc tctgtcgccg
    45661 gaacaatgat cttgcctacg cccgcttgtg ccgcgcgttg caggctggcc tcttcatcgc
    45721 caataaaagg cgggaaatca aaatggcagt gagtgtcgat aaaacggcaa atcaagccag
    45781 atcctcattg ttaaatgtcg ggtcgttcgc ctgcggagcg tcgctcactt cggcaaccag
    45841 cgtatcgttg gcgactggcg caggcggaat gacaatcgat tccggcgtca ccactggcac
    45901 cagatggcgg gtaagcggcg ctttttcagt taatagcttg ccaaccgtgg cgaggaaata
    45961 acgaccgcat aaacgcccaa gtttatagtc ttcacgcagc gccggaatcc gactgccaag
    46021 cgcgatacta tgtaatggct tcggcggata aatttcgaat atccgcagct tgcccggtgg
    46081 tttctcaata aattgctgaa tgtcacgata gctggtttca tgatgctgca ccagattgac
    46141 cagcggctgc aggctactgt cacccagcca gcgttccatg cgtttgaacc actgcggcgt
    46201 gtagtacatt tgtgacggca cagtgcggat gacgaccaac gttttagcgc cctgccttgc
    46261 cgcctcttta accggaatcg catcactgat cccgccatcc aggtagttaa tgccttccag
    46321 cgacactccg ctacgataaa agccaggtat cgcactggag gcgcgaatca catccagcca
    46381 gttttgtttg gttggtaaaa agtaattcgg cgcgtagtca tcctgacgac aggcgcacat
    46441 ataaaacgat ttgccgctgt caaacaaccg cgcggcggta tccatttgca acggcatctg
    46501 gcttgcggtg gcctccacca gccagtcgag atcgataaga tttcctccac ggacaaagcg
    46561 caatggatcg aaaaattcgc gttttgtggt atagcgcatg atgactttgc gcgcgtaacc
    46621 gggctgattg cagataaacg ccgagaggtt ctgcgccccg gcagatgtgc cgagataaag
    46681 atcgaaagga ttaaactgcg cgcgcataaa ctcatccagc acgccagccg tgaaaattcc
    46741 acgctgtccg ccgccttcgc acaccagagc tattcgtcca ggctggaacg gccttagcga
    46801 caacggcgca atattaccaa gcgttacagg tattcgctgc cccacttctg ccttcctgtt
    46861 tttgttgtga aagaaagaca aaataacgca atttattatt cgctaaaacc gcaaaagcca
    46921 gtccgctgga ctggctttgg ataaaatttt cttaatggtc tgaaatctag ggtcgttttc
    46981 ggcccatgaa caaactcacc aggaacagaa taatcccgac gacaaagaca attttagctg
    47041 cgcctgcagc ggtaccggcc agaccaccaa acccaagtgc ggcggcgatt aacgcgataa
    47101 ccagaaatat gatgccccaa cgaaacataa gcgtctcctt taccatagtt aatgtcaccg
    47161 cttgatatga gcgtgaaaat cactcaatgc gatattttta gtgtggtgca catcgcgcct
    47221 cccgacaaag ttcgggagga cgaattacga cgaattactt agttttcaga tcatttttaa
    47281 cgcttttcac accatctacc gctttggcga tactttcagc acggtcactt tgtgcctgag
    47341 aatctacggt accggagagc tgaaccacgc catcggtggt ttcaactttc actttacgtg
    47401 aagggacgat atcgtccgcc agcagtttgg ctttgatttc actggtggtg gcggtgtcac
    47461 ccgcgtagcc cttcaccgag ccttctttag cgtcgcgaac gtgcagtttg tcgctgacag
    47521 aggtcacgcc ttcaacgcct ttcgccactt tcactgcctc ttcggcctgg gcctgacttt
    47581 caacgaaacc gctcagggtc acgacttttt gatcggtttt gacagagata tcggtgctct
    47641 tgatgttgtc atgatccacc agcgccgcct tcactttcgc ggtgatggcg ctgtcatcca
    47701 tgaaattacc gactttattc atagagctat cgactttttg ccctgcgctt tcattggtag
    47761 tctgcgcgtt gttttccgcg taggcagagc cggtcgcgac ggcagaggtc aacattacag
    47821 ccagcagagt tttcgaaatc ttcagtcttg tcatagtcat cgatttattc ctgtatgttt
    47881 gctcgtaatt tgagcctggc aacacgaggt tgcattgctg aatagggaga gacttcaccc
    47941 tctacagaag tcaatggtcg ccatcacaaa agcgatgggt gatgaataac gaccattaca
    48001 gcctctgaat cagttattaa tatcggtaga atgacaatcg acggctttag atactgatat
    48061 ctacgcattg aacggtattt aacgccatca gaaatgtcat cactttgtta aatatagatc
    48121 acaattttga aaccgctcgg gatatcagcg aaaacataag caaaagtgaa tgttttaaga
    48181 acattccgta agcggctaat aaggaaggga aattgacagg gcgcagcggc taccgcgccc
    48241 tggaggcaag aattaatgct cacgggtctg gtggaactga acgtccggat aacgttcctg
    48301 tgccaggcgc aggttgacca tgctggtagc gatgtaagcg aggttatcgc cgccatcaag
    48361 cgccagttgg ctttcgttct tacgcttgaa ctcttcgaat ttcttcgcgt ctgcacattc
    48421 tacccagcgg gcagtggcaa cgttgactga ctcatacact gcctcaacgt tgtattcgct
    48481 cttcaggcgc gctaccacca catcaaactg cagcacacca accgcaccaa cgatcagatc
    48541 gttgttggag attggacgga acacctgcac cgcgccctct tcggaaagct gtaccagccc
    48601 tttgagcagc tgtttttgct tcagcggatc tttcaggcgg atacgacgga acagttctgg
    48661 tgcgaagttc ggaataccgg tgaacttcat catctcaccc tgggtaaagg tgtcgccgat
    48721 ctgaatggtg ccgtggttgt gcaggccgag gatatcgccc ggatacgctt cttcaacgtg
    48781 cgaacggtca cccgccataa aggtcagcgc gtcggagatc accacatctt tcgcagtgcg
    48841 cacctggcgc agtttcatgc ctttttcata tttaccggac accacacgca taaacgccac
    48901 gcggtcgcgg tgtttcgggt ccatgttggc ctgaatttta aatacgaagc cggtaaattt
    48961 atcttcgctc gcttctacgg tacgggtatc ggtctgacgc ggcatcggcg caggcgccca
    49021 ctccaccagg ccatccagca tatgatcaac gccgaagtta cccagcgcag taccgaagaa
    49081 taccggagtg atttcgcccg caaggaacag ctctttgtcg aactcgttag acgcgccttt
    49141 aaccagttcc agttcgtcac gcagctgctg tgccagatct tcaccaaccg cagcatcgag
    49201 atccgggtta ttcagccctt taacaatgcg gacttcctga atggtgtgcc ctttaccgct
    49261 ctgatagaga taggtttcat ctttataaag gtggtaaacg cctttaaaca gcttgccgca
    49321 gccaattggc caggtgatcg gtgcgcagcc aattttcagc tcgttctcaa cttcatcgag
    49381 cagttccatc gggtcgcgga tatcacggtc aagtttgttc ataaaggtga ggatcggcgt
    49441 gtcgcgcaga cgggtaactt ccatcagctt acgggtacga tcttcaacac cttttgcggc
    49501 gtcgataacc atcaggcagc agtccaccgc cgtcagggta cgataggtat cttccgagaa
    49561 gtcttcgtgc cccggggtgt cgagcaggtt aaccaggcaa tcgtgatacg gaaactgcat
    49621 cacagacgta gtaatggaga tcccacgctg cttttccatc tccatccagt ccgacttagc
    49681 gtgctggttg gaaccacggc cttttactgt accggcggtc tgaatggcct gtccgaacag
    49741 cagcaccttc tcggtgatgg tagttttacc ggcgtccggg tgagaaataa tggcaaaagt
    49801 gcggcgcttc gccacctctt gcaaataagg agacaacgtc ataattcttt cttcttgagt
    49861 aagcggcatc gcgccgcgct tgttgaaaac gaaaaattgc ggctatttta cccatcaacg
    49921 ggggggaggc aatcagtgtt tacacaggag ctgctccagt tcgtgcaacg aagaaacggt
    49981 ccaggtgggc gcgatgcctt ctggttgctc gcgattgtgc gcattcagcc agcaggtcgc
    50041 aagcccggcg ttgatgccac cgagaatatc ggactcggca gtgtcgccaa ccatcagcac
    50101 gcgtgaacgg tcaggattgc ccgcctgttc cagcgcataa tcgaaaattt tcttattcgg
    50161 tttggcaacg ccaacttctt cggaaatcac cagcaaatcg aagtaatcac gcaggcccgt
    50221 ccgttccaga cgcacttgct gcaaggcgct aaaaccgttg gtgatgatgc cgattttagc
    50281 gttgccacga atggcgttaa gcagagaaac cgcgcccggc aacggcgtac agatttccgc
    50341 catcgcatta ataaaggcat cgttaagcaa gcctgcttca actttcagcc gttcggccca
    50401 gctctcgaaa cgcccgtgct gaagctgtaa tgaagtgatc gcgccgtttt gataatccac
    50461 ccacagtggc ttgttaacgg cctgatagtc ctgaaaatct tcagcggtaa aggtgacgct
    50521 gtaatcaaga aacatccgct gcaggccggt gaatgagtca aaggtaaaca gcgtttcatc
    50581 ggcatcaaag aaaatccagt cccacttcat tattccacct tgtcttacat actgattggc
    50641 aacgccatga tgatggcgtc ttcgcgaccg tccgtggtgg ggtagtaatt gcggcgaatc
    50701 gtcgcctcgt taaagcctaa actttcgtac agggcaatgg cggcagcatt tgaggcacgg
    50761 acttccagcc atagtgtcgc cacgccgcgt ttttccagtt cgtcgatcag atgttccagc
    50821 agcgccctac ccagcccctg acgctgatag tcgggatcga ccgcaatatt gaacaatgta
    50881 gcttcatcca gcaccacttg cgtaatcgca aacgccgcca ttttgccgtt ttgcgttaac
    50941 tgaaagttga gataacgctc gccctggttg ctggcaaagg ttttttcact ccacggaaag
    51001 gcgtgggcgc gttgttcaat gtggtaagcc gctggtaaat cagtcgtttc gagggaagaa
    51061 atcgtgttca tatgtgcaaa tttgttgcca taacgcggcg cgtgccgttg ggtttgcccg
    51121 taattcggtg agcgccggtg atgccacctg agcgccttcc aatgatagcg gttcgtcagt
    51181 acccaaccgc cagctgttgc agcgactgcc ttgcggcagc atcgcgattt tttctggcgt
    51241 cagttgcagc acttgatcgg ggctgacggt taatgcgcgc agaacatcgc tcactaaagg
    51301 atcagtcagg gcgggaagat cgtttgccac catcaccaga cggacgtgtg ccgggctcgc
    51361 aatggcaatc tccccctgca acgcgccagg gcgacgcagc gaccactggg taatgcccag
    51421 ttgctgtaac tgccagtctc gtcgggatgt catagcgaat cgctcctgtt gtcaggggcg
    51481 caaatatagc aaattcgtcg ataccgcgcc aacatatggc tataatcgcc gccagtatca
    51541 attgaggagc attccatgtc tgcatttacc ccggcaagtg aagtcttgct gcgtcacagt
    51601 gatgatttcg aacaaagccg tattctgttt gccggagact tacaggatga cctgcccgcg
    51661 cgtttagata ccgcggccag ccgtgctcat acccagcaat tccaccattg gcaggtgtta
    51721 agctgccaga tgggggataa cgcccgtttt agtctggtcg ccacggcgaa tgatgtcgca
    51781 gattgcgata cgctgattta ctactggccg aagaacaaac cggaagccca gttccagttg
    51841 atgaatttac tttctctgct gccagtgggg acagatattt ttgtcgttgg cgagaaccgc
    51901 agcggcgtgc gcagcgctga gcagatgctg gcagattatg cgccgttgaa taaagtcgac
    51961 agcgctcgtc gctgtggcct ctattttggt cgtctggaaa aacagccagt atttgatgcg
    52021 gataaattct ggggcgaata cagcgtcgat ggcctgacgg tcaaaacgct gcctggcgtg
    52081 tttagccgcg acggtctgga tgtcggtagc cagttgctgc tctcgacgtt aactccgcac
    52141 acgaaaggta aagtgctgga tgtcggctgt ggcgcgggtg tactttcagt tgcctttgcg
    52201 cgccattcgc cgaaaattcg tctcaccttg tgcgatgtct ctgccccggc ggtagaagcc
    52261 agccgcgcaa cacttgcagc caacggtgtt gaaggtgaag tctttgccag caacgtcttt
    52321 tccgaggtga aaggtcgttt tgatatgatc atctccaacc cgccgttcca cgatgggatg
    52381 caaaccagcc tggatgcggc gcaaacgctg attcgcggcg cggtgcgtca tcttaatagc
    52441 ggcggcgagc tgcgaattgt agcgaacgcc ttcctgcctt acccggacgt gctggatgag
    52501 acatttggct tccatgaagt tatcgcgcaa accgggcgct tcaaggtata tcgcgccatt
    52561 atgacccgcc aggcgaagaa aggttgagta tctgatatct gtcgccgcat accgtgtcgg
    52621 atgcggcgtg aacgccttat ccgacctaca aaacctgcac gttagccctt cgtaggccag
    52681 ataagacgcg ccagcgtcgc atctggcatc tgcccccgca tgcctaccaa acctgcacgc
    52741 tagtttcctg atggacattt ttccagcaat tacacatcag gcgataatta actattgacg
    52801 aaaagctgaa aaccactaga atgcgcctcc gtggtagcaa ttctttttaa gaattgatgg
//
LOCUS       DADLSB010000010        50019 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.010, whole genome shotgun sequence.
ACCESSION   DADLSB010000010 DADLSB010000000
VERSION     DADLSB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.010"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..318)
                     /locus_tag="JN331_000599"
     CDS             complement(<1..318)
                     /locus_tag="JN331_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY7258828.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIH"
     gene            complement(335..595)
                     /locus_tag="JN331_000600"
     CDS             complement(335..595)
                     /locus_tag="JN331_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7258829.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(586..1856)
                     /locus_tag="JN331_000601"
                     /pseudo
     CDS             complement(586..1856)
                     /locus_tag="JN331_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(1934..2299)
                     /gene="queD"
                     /locus_tag="JN331_000602"
     CDS             complement(1934..2299)
                     /gene="queD"
                     /locus_tag="JN331_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY7258830.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            2615..4414
                     /gene="cysJ"
                     /locus_tag="JN331_000603"
     CDS             2615..4414
                     /gene="cysJ"
                     /locus_tag="JN331_000603"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY7258831.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            4414..6126
                     /gene="cysI"
                     /locus_tag="JN331_000604"
     CDS             4414..6126
                     /gene="cysI"
                     /locus_tag="JN331_000604"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY7258832.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            6200..6934
                     /gene="cysH"
                     /locus_tag="JN331_000605"
     CDS             6200..6934
                     /gene="cysH"
                     /locus_tag="JN331_000605"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7258833.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            7198..7350
                     /locus_tag="JN331_000606"
     CDS             7198..7350
                     /locus_tag="JN331_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7258834.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            7544..8695
                     /locus_tag="JN331_000607"
     CDS             7544..8695
                     /locus_tag="JN331_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY7258835.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGSDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(8781..9818)
                     /gene="iap"
                     /locus_tag="JN331_000608"
     CDS             complement(8781..9818)
                     /gene="iap"
                     /locus_tag="JN331_000608"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY7258836.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTHDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10070..10978
                     /gene="cysD"
                     /locus_tag="JN331_000609"
     CDS             10070..10978
                     /gene="cysD"
                     /locus_tag="JN331_000609"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY7258837.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVTL
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10980..12407
                     /gene="cysN"
                     /locus_tag="JN331_000610"
     CDS             10980..12407
                     /gene="cysN"
                     /locus_tag="JN331_000610"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY7258838.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSLGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            12407..13012
                     /gene="cysC"
                     /locus_tag="JN331_000611"
     CDS             12407..13012
                     /gene="cysC"
                     /locus_tag="JN331_000611"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY7258839.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13062..13385
                     /locus_tag="JN331_000612"
     CDS             13062..13385
                     /locus_tag="JN331_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY7258840.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            13579..13890
                     /gene="ftsB"
                     /locus_tag="JN331_000613"
     CDS             13579..13890
                     /gene="ftsB"
                     /locus_tag="JN331_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY7258841.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMIRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            13909..14619
                     /gene="ispD"
                     /locus_tag="JN331_000614"
     CDS             13909..14619
                     /gene="ispD"
                     /locus_tag="JN331_000614"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY7258842.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSENSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            14619..15098
                     /gene="ispF"
                     /locus_tag="JN331_000615"
     CDS             14619..15098
                     /gene="ispF"
                     /locus_tag="JN331_000615"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY7258843.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTFGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15095..16144
                     /gene="truD"
                     /locus_tag="JN331_000616"
     CDS             15095..16144
                     /gene="truD"
                     /locus_tag="JN331_000616"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY7258844.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            16125..16886
                     /gene="surE"
                     /locus_tag="JN331_000617"
     CDS             16125..16886
                     /gene="surE"
                     /locus_tag="JN331_000617"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY7258845.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            16880..17506
                     /gene="pcm"
                     /locus_tag="JN331_000618"
     CDS             16880..17506
                     /gene="pcm"
                     /locus_tag="JN331_000618"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY7258846.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            17646..18785
                     /gene="nlpD"
                     /locus_tag="JN331_000619"
     CDS             17646..18785
                     /gene="nlpD"
                     /locus_tag="JN331_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY7258847.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            18848..19840
                     /gene="rpoS"
                     /locus_tag="JN331_000620"
     CDS             18848..19840
                     /gene="rpoS"
                     /locus_tag="JN331_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY7258848.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSCMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(19960..20367)
                     /locus_tag="JN331_000621"
     CDS             complement(19960..20367)
                     /locus_tag="JN331_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY7258849.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            20514..21107
                     /locus_tag="JN331_000622"
     CDS             20514..21107
                     /locus_tag="JN331_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7258850.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            21107..22534
                     /locus_tag="JN331_000623"
     CDS             21107..22534
                     /locus_tag="JN331_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAY7258851.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTTMLAARK"
     gene            22545..22781
                     /locus_tag="JN331_000624"
     CDS             22545..22781
                     /locus_tag="JN331_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258852.1"
                     /translation="MICPRCADKQIEVMVKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(22821..24185)
                     /locus_tag="JN331_000625"
     CDS             complement(22821..24185)
                     /locus_tag="JN331_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HAY7258853.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSCGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGVVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTGDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(24280..25050)
                     /locus_tag="JN331_000626"
     CDS             complement(24280..25050)
                     /locus_tag="JN331_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAY7258854.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYNYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDA"
     gene            complement(25055..25693)
                     /locus_tag="JN331_000627"
     CDS             complement(25055..25693)
                     /locus_tag="JN331_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7258855.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(25690..26952)
                     /locus_tag="JN331_000628"
     CDS             complement(25690..26952)
                     /locus_tag="JN331_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAY7258856.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMHHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVAHCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(26949..27721)
                     /locus_tag="JN331_000629"
                     /pseudo
     CDS             complement(26949..27721)
                     /locus_tag="JN331_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            27887..28684
                     /locus_tag="JN331_000630"
     CDS             27887..28684
                     /locus_tag="JN331_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7258857.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(28735..29391)
                     /gene="pphB"
                     /locus_tag="JN331_000631"
     CDS             complement(28735..29391)
                     /gene="pphB"
                     /locus_tag="JN331_000631"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY7258858.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     CPVYRMQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(29497..32058)
                     /gene="mutS"
                     /locus_tag="JN331_000632"
     CDS             complement(29497..32058)
                     /gene="mutS"
                     /locus_tag="JN331_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY7258859.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNGPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            32134..32313
                     /locus_tag="JN331_000633"
     CDS             32134..32313
                     /locus_tag="JN331_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258860.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            32345..32698
                     /locus_tag="JN331_000634"
     CDS             32345..32698
                     /locus_tag="JN331_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY7258861.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(32735..34803)
                     /gene="flhA"
                     /locus_tag="JN331_000635"
                     /pseudo
     CDS             complement(32735..34803)
                     /gene="flhA"
                     /locus_tag="JN331_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(34877..35887)
                     /gene="hypE"
                     /locus_tag="JN331_000636"
     CDS             complement(34877..35887)
                     /gene="hypE"
                     /locus_tag="JN331_000636"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY7258862.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(35884..37005)
                     /gene="hypD"
                     /locus_tag="JN331_000637"
     CDS             complement(35884..37005)
                     /gene="hypD"
                     /locus_tag="JN331_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY7258863.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(37005..37277)
                     /gene="hypC"
                     /locus_tag="JN331_000638"
     CDS             complement(37005..37277)
                     /gene="hypC"
                     /locus_tag="JN331_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY7258864.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(37268..38140)
                     /gene="hypB"
                     /locus_tag="JN331_000639"
     CDS             complement(37268..38140)
                     /gene="hypB"
                     /locus_tag="JN331_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY7258865.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYRHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(38144..38494)
                     /gene="hypA"
                     /locus_tag="JN331_000640"
     CDS             complement(38144..38494)
                     /gene="hypA"
                     /locus_tag="JN331_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7258866.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKCVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            38706..39167
                     /gene="hycA"
                     /locus_tag="JN331_000641"
     CDS             38706..39167
                     /gene="hycA"
                     /locus_tag="JN331_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY7258867.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            39292..39903
                     /locus_tag="JN331_000642"
     CDS             39292..39903
                     /locus_tag="JN331_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7258868.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            39900..41724
                     /gene="hycC"
                     /locus_tag="JN331_000643"
                     /pseudo
     CDS             39900..41724
                     /gene="hycC"
                     /locus_tag="JN331_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            41727..42650
                     /gene="hycD"
                     /locus_tag="JN331_000644"
     CDS             41727..42650
                     /gene="hycD"
                     /locus_tag="JN331_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY7258869.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            42668..44377
                     /locus_tag="JN331_000645"
     CDS             42668..44377
                     /locus_tag="JN331_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY7258870.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFSNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDGMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            44387..44929
                     /gene="hycF"
                     /locus_tag="JN331_000646"
     CDS             44387..44929
                     /gene="hycF"
                     /locus_tag="JN331_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY7258871.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            44929..45696
                     /gene="hycG"
                     /locus_tag="JN331_000647"
     CDS             44929..45696
                     /gene="hycG"
                     /locus_tag="JN331_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY7258872.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            45693..46103
                     /locus_tag="JN331_000648"
     CDS             45693..46103
                     /locus_tag="JN331_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY7258873.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            46096..46566
                     /gene="hycI"
                     /locus_tag="JN331_000649"
     CDS             46096..46566
                     /gene="hycI"
                     /locus_tag="JN331_000649"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY7258874.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            46609..47360
                     /locus_tag="JN331_000650"
                     /pseudo
     CDS             46609..47360
                     /locus_tag="JN331_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            47533..47844
                     /locus_tag="JN331_000651"
     CDS             47533..47844
                     /locus_tag="JN331_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY7258875.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            47841..48260
                     /locus_tag="JN331_000652"
     CDS             47841..48260
                     /locus_tag="JN331_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7258876.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(48375..49799)
                     /locus_tag="JN331_000653"
     CDS             complement(48375..49799)
                     /locus_tag="JN331_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7258877.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(49808..>50019)
                     /locus_tag="JN331_000654"
     CDS             complement(49808..>50019)
                     /locus_tag="JN331_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7258878.1"
                     /translation="GLFTSVQFFDPANPMSIVWVFAVMALAVMLSFILTLLLGFEDIP
                     VEEAAAQARKHQSVQPTVAKEVSLN"
BASE COUNT        11805 a        12977 c        13456 g        11781 t
ORIGIN      
        1 atgaatacag aaaaaaactt ccgctcttgc cgcttttagc atcgatgctt tggtgctgat
       61 aattccatcc gcctcggtca ccagtttcaa aaactgaatc accacttctt tatttgacgc
      121 gccttccagc aaatcaacat gaataaaagc atatttcccg gcgttcttaa tctttttaac
      181 gatattactg atggtgcaga tattgccgta caacacggaa ataaactggc attcagaatc
      241 gatggccagt tgcaggctgg cattatcttt aacggcagca atcaccggat tctggcggag
      301 caggtgtaag aggggcataa attatcctta ttatttatcc aaagcggtag gtgatgccga
      361 atccggaagg cggatagcgc cactgttgta gtgtggattc gtcgcacagc aaacggcagg
      421 tgccacactc caggcagccg cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa
      481 aaagacctac cggacaggcg ttaatcaacc gttgtaccat ctgacgctca acggagtcag
      541 ccggaacaat gtgcggcgca tcagcaacgc gccagagatt acgggctaca gacatcgcag
      601 actcctgata acatcgcccg ccagatgcca caggccatga cgacgtaaat gacgccagaa
      661 taactggcgc agcggtggaa caggtttatc accctgatcc cataaatcgc gggaaatatc
      721 ctgcattaac gcaggccacg cccgatacca gccaggggtt gcaaaagcgc cggaacatgc
      781 tgataacgtt gtagaacatc ccatagcagg ctgcgctcta cgttgtgatg ataaagcgca
      841 aacagatttt gcggctcgcg gtgctggcag gcgcttatta gcgtttgtgc cgccgcctgt
      901 gcgccagtca gcgccatatc catgccgcgc acggaaattc cggtattgac gcaactgcgc
      961 aacgcatcgc ccaccagcag ccagccgtta ccggcgtatt gcaccggcat actgtgcaag
     1021 ccaccttctg gcaccagatg cgcaccatac tccagtgatt ccgtgttttt gataagcggg
     1081 cgcactgccg gatgcgcttt aaagcgagtc agcagctcgc ttgccggaac acgactttgc
     1141 gtaagggaag agagcgggca aacaatccct aacgagagcg tttgttgatt agtataaaga
     1201 aatgcgccgc cgggtaagtc atcacagatt ccgccgctga acagcaacgc tgcgccttcg
     1261 ttattctcca gatgaaaacg ttcttcaata gcggatgttt ccagcgacag cacttctttg
     1321 atccccaacg ccatcgcttc gccagcagga cgagtcacta acccgtgacg ttcagccagg
     1381 acgctgttgg caccttctgc cagcaccaca taacgggcgc ggagaatatc gtcaccacaa
     1441 ataacgccac agactctgcc gttttcttca tacagtgcat ccaccgtcgc tccggggatg
     1501 cattcgacac cttctttttc ggcttcggca accagccacg gatcgaaccg tgcacgtaat
     1561 acactccagg attcaccgcc gggctgtaag ctggaaaacg tcgttgcgcc atccggcgtt
     1621 aacagggaaa ggctttcgtg agtgatgcgt cgttcaagag gcgcggtcag atgaaattgc
     1681 gggaggagtt ccgcgagtgc atgggtatat aaccgcccgc cggaaagatt tttgctgccg
     1741 gggatttcag cgcgttcgag taacaaaacg gataaatccg ctcgcgcgca gcgtaacgcg
     1801 caagcggtgc ctgcaatacc ggcaccaata ataataatgt cgcagtcgtc ttccatgtca
     1861 tgccccagat atccagaaca caacacccta acatagcgtt actaaaggga aattgaccgc
     1921 cgacacactc ttatcattcg ccgcgataga tacaccctgc ggtgcaggtt tcttttacca
     1981 tcaccgcact taacagcggc acaacgggtt taacctgatc ccaaatccat tttgctaaaa
     2041 cttcgctggt tgggttttcc agacctggaa tatcattgag ataatggtga tcgaggcgct
     2101 cataagttgg tttaaacgcc gctttcagtt cagcgaaatc gataatccag ccagtatgcg
     2161 gatcgacttc cccggtaatt tccagtcgca ccataaagga atgcccgtgc aggcgaccac
     2221 atttatgccc ttccgggaca tgtggtaagc ggtgagcggc ttcgaaggtg aaatctttaa
     2281 ataacgtggt ggacatcata atttctctac aggacgataa aaccgccgta gagtaccgga
     2341 aaggctgttt tttcgcatta tctaaaacga ctttgttgcg caaaatcgct gatttatctt
     2401 aatgattggc taaattcatt tgtttttcat taggttggtt aatctatttt gttgttaaag
     2461 actattgcta aaacaggtta gtcgatttgg ttattagtta tcgctatccc gtctttaatc
     2521 cacaccgttc accccgttaa ccttaccttc tcttctgttt tatgggcgct gacagggcgc
     2581 agaaacagct ttgcttactg gaacataacg acgcatgacg acacaggtcc caccttccgc
     2641 gttgcttccg ttgaacccgg agcaactggc acgccttcag gcggccacga ccgatttaac
     2701 gcctacccag cttgcctggg tttctggcta tttctggggc gtgctcaacc agcagcctgt
     2761 tgcgcttgca gtgacgccag cgcccgccgc agaaatgccg ggtataacga ttatctctgc
     2821 ttcgcaaacc ggcaatgcgc gccgggttgc tgaagcatta cgcgatgatt tattagcagc
     2881 aaaactgaac gttaagctgg tgaacgcggg cgactataaa ttcaaacaaa tcgccagcga
     2941 aaaactgctc atcgtagtga cgtcaacgca aggggaaggg gaaccgccgg aagaagccgt
     3001 cgcgctgcat aagttcctgt tctccaaaaa agcgccaaag ctggaaaaca ccgcgtttgc
     3061 cgtgtttagc ctcggcgata gctcttatga atttttctgc cagtccggga aagattttga
     3121 cagcaagctg gcggaactgg gtggtgaacg cctgctcgac cgtgtcgatg ctgatgttga
     3181 ataccaggct gctgccagcg agtggcgcgc ccgcgtggtt gatgcgctta aatcgcgtgc
     3241 gcctgtcgcg gcaccttcgc aatccgtcgc tactggcgcg gtaaatgaaa tccacaccag
     3301 cccgtacagc aaagacgcgc cgctggtggc tagcctctct gttaaccaga aaattactgg
     3361 gcgtaactct gaaaaagacg ttcgccatat cgaaattgac ttaggcgact cgggcctgcg
     3421 ttaccagccg ggtgacgcgc tgggcgtctg gtatcagaac gatccggcac tggtgaaaga
     3481 acttgtcgaa ctgctgtggc tgacaggcga tgaacctgtc actgtcgagg gcaaaacgtt
     3541 gcctctgaac gaagcgctac agtggcactt cgaactgacc gtcaacaccg ccaacattgt
     3601 tgagaattac gccacgctta cccgcagtga aacactgctg ccgctggtgg gcgataaagc
     3661 gaagttacag cattacgccg cgacgacgcc gattgttgac atggtgcgtt tctccccggc
     3721 acagcttgat gccgaagcgc taattaatct gctgcgcccg ctgaccccgc gtctctattc
     3781 cattgcctcc tcgcaggcgg aagtcgagaa cgaagtacac gtcaccgttg gtgtggtgcg
     3841 ttacgacgtg gaaggccgcg cccgtgccgg tggtgcctcc agcttcctcg ctgaccgcgt
     3901 ggaagaagag ggcgaagtcc gcgtatttat cgaacataac gataacttcc gcctgcccgc
     3961 taacctggaa accccggtga ttatgattgg cccaggcacc ggtattgcgc cgttccgcgc
     4021 ctttatgcag caacgcgccg ctgacgaagc gccaggtaaa aactggctgt tctttggcaa
     4081 tccgcatttt acggaagact tcctgtatca ggtggagtgg cagcgctacg tcaaagaggg
     4141 tgtgctgacg cggatcgatc tcgcctggtc gcgcgaccaa aaagaaaaag tttacgtaca
     4201 agataaactg cgcgaacagg gcgcggagct gtggcgctgg atcaatgacg gtgcccacat
     4261 ttatgtctgc ggcgacgcta atcgcatggc gaaagacgtt gagcaggcac ttctggaagt
     4321 gattgccgaa tttggtggca tggacaccga agcggcggat gaatttttaa gtgagctgcg
     4381 cgtagagcgt cgttatcagc gagatgtcta ctaatgagcg aaaaacatcc agggccttta
     4441 gtggtcgaag gaaaactgac agacgccgag cgcatgaagc ttgaaagcaa ctacctgcgc
     4501 ggcaccattg cggaagattt aaacgacggt ctgaccggcg gctttaaggg cgataacttc
     4561 ctgctgatcc gcttccacgg catgtatcag caggatgacc gcgacatccg cgccgaacgt
     4621 gctgaacaga agctggagcc gcgccacgcg atgctgttgc gctgccgtct gccgggtgga
     4681 gttatcacca ccaaacagtg gcaggcgatc gataaatttg ccggtgaaaa caccatctat
     4741 ggcagcattc gcctgaccaa ccgccagacg tttcagttcc acggcattct gaaaaagaac
     4801 gtcaaaccgg tgcaccagat gctgcactcg gtcggtcttg atgcgctggc gactgctaac
     4861 gacatgaacc gtaacgtact ctgcacctcg aacccttacg agtcgcagct gcacgcagaa
     4921 gcgtacgagt gggcgaagaa gatttctgaa catctgctgc cgcgtacccg cgcgtatgcg
     4981 gagatctggc tcgatcagga aaaagtcgcc accaccgatg aagaaccgat ccttggtcag
     5041 acctacctgc cgcgtaagtt caaaaccacg gtagtgatcc cgccgcagaa cgatattgat
     5101 ctgcacgcca acgacatgaa cttcgtggcg atcgcggaaa acggcaagct ggtgggcttc
     5161 aacctgctgg tgggcggtgg gctttctatc gaacacggta acaagaaaac ctacgcccgc
     5221 acggcgagtg agtttggcta tctgccgcta gagcatacgc tagcggtggc ggaagccgtc
     5281 gtgacaactc agcgtgactg gggtaaccga accgatcgta aaaatgccaa aaccaaatac
     5341 acactggagc gcgtgggggt tgagacgttt aaagcggaag tggagcgtcg cgcggggatc
     5401 aaatttgaac cgatccgccc gtatgaattt acagggcgcg gcgatcgtat tggctgggtt
     5461 aagggcattg atgataactg gcacctgacg ctgtttatcg aaaatggtcg catccttgat
     5521 tatccggggc gtcctctgaa aactggcctg ctggagatcg cgaagatcca caaaggtgat
     5581 ttccgcatta cggcgaacca gaatctgatc atcgccggtg tgccggaaag cgagaaagcg
     5641 aagatcgaga aaattgccaa agagagcggg ttaatgaatg ccgtcacgcc gcagcgtgaa
     5701 aactcgatgg cctgcgtgtc attcccgact tgcccgctgg cgatggcgga agcggagcgt
     5761 ttcctgccgt cttttatcga caacatcgat aatttaatgg cgaaacatgg tgtcagcgat
     5821 gagcatatcg tgatgcgtgt aacaggctgc ccgaacggtt gtggtcgcgc gatgctggcg
     5881 gaagtgggcc tggtgggtaa agcaccgggt cgctacaacc tgcatcttgg cggcaaccgc
     5941 attgggacac gtatcccacg gatgtataaa gaaaacatca ccgagccgga aatcctggcg
     6001 tcgcttgatg aactgatagg gcgctgggcg aaagagcgcg aagcgggtga aggcttcggc
     6061 gactttacgg tgcgtgcggg catcattcgc ccggtgctcg atccggcgcg cgatttgtgg
     6121 gattaaccat cagcccggtc ttgtaggcct gataagacgc gtgagcgtcg catcaggcaa
     6181 ggcaaacagt gaggaatcta tgtccaaact cgatctaaac gccctgaacg aactgccgaa
     6241 ggtagatcgc attctggcgc tggcagaaac taacgccgaa ctggaaaaac tggacgctga
     6301 aggccgcgtt gcctgggcgc tggataatct gcccggtgaa tatgtacttt cttccagctt
     6361 tggcattcag gcggcggtga gcctgcatct ggtgaatcag attcacccgg atattccggt
     6421 gatcctcacc gacaccggct atctgttccc ggaaacctac cgctttattg acgagttaac
     6481 ggacaaactc aagctcaacc tgaaagtgta ccgcgctacc gaaagcgccg cctggcagga
     6541 agcacgctac ggcaaactgt gggaacaggg cgttgaaggc attgaaaagt acaatgacat
     6601 caacaaagtc gaaccgatga accgggcgct gaaagaactg aatgcgcaaa cgtggtttgc
     6661 tggcctgcgg cgtgaacaat ccggcagccg tgccaattta ccggtgctgg caattcagcg
     6721 tggcgtattt aaagtgctgc cgattatcga ctgggataac cgaactattt atcagtacct
     6781 gcaaaaacat ggcctgaaat atcacccatt atgggacgaa ggatatttat cggtgggcga
     6841 tacccataca acccgtaaat gggaacccgg catggcggaa gaagaaacgc gtttctttgg
     6901 cttaaaaagg gaatgtggat tacacgaagg gtaaatattg ccggataaat taaacgcctc
     6961 tgtcagaaat gatgggggcg tttttatttt gcgagcagca ttccggcatt taaaggtgaa
     7021 catcgcaggg aaaattaaca ggtatagaat ggcgttgggt gcttgaggct gtctggcgtt
     7081 aagcattcgc gaggttccag atggacaaaa gccccaggcg atatttctat caacctgagg
     7141 ctcgcgttcg aacgtagcaa ttcggatgtt agtctcttct ttgcagggag gcaagacatg
     7201 ctgacaaaat atgcccttgt ggcaatcatc gtactgtgtt gtacagtact gggattcacg
     7261 ttgatggtag gtgactcgtt atgtgagttg agtatcagag aacgtggtat ggagtttaag
     7321 gcagttctcg cttacgaatc gaagaagtag ccaccacgcg ggggtaaaac cccgcgtact
     7381 gaattgtgat ggtttggtct cctgcaccct tttttaaaat ttatattttt tatgtgaata
     7441 tatagtctct atttattatt atgaatttca aatgaaaatc attaatttat tcataatgta
     7501 aatgttttat tggctgatat atatcacaga taatttttaa aatatgcgta aatatccttt
     7561 aagtttactg aaggataaaa atattgtgac tttctttgat ttctggggaa aaacccgacg
     7621 tggcgagaaa gagggtagcg acgactatca ccttctttgc tggcatttgc tggatgtggc
     7681 cgcaatgggc tatttaatgg ttaaaagaaa ttgcttcggg ctggctgatt attttcgtca
     7741 attagggatt tctgacaagg aacaggcagc tcaatttttc gcctggttgc tgtgctggca
     7801 cgatattgga aaatttgccc gctcttttca acaactttac ctgccccctg aattcaagat
     7861 tcaggaaggt gccagaaaaa attacgaaaa aatctctcat tcaacgctgg gctactggtt
     7921 gtggaatcat tatttaagtg aatgtcagga gttacttcct tcatcttcgc tctctccgcg
     7981 caaacttagg cgagtgatag aaatgtggat gcccgtgacg acagggcatc atggtcggcc
     8041 ccctgatcgt atggatgaat tggataattt tctgcctgaa gacaaagctg cagcacgaga
     8101 ttttctcctt gaaatcaagc cactgtttcc gcgaatagag atccccacat tctgggatga
     8161 tgacgagggc gttgaactta taaaacaact ttcctggtat atctctgcaa ccgtcgtact
     8221 tgcagactgg acagggtcgt caacgcgatt ttttccacgc gtcgcacagg caatggatat
     8281 tgaagattac tggcaaaagg ctttagttca ggctcaaaac gcattaaccg tctttccgcc
     8341 acaagcagaa accgcgcctt tcaccggaat taatacgctg tttcctttta ttgagaatac
     8401 gacaccgtta cagcaaaagg tgctggatct ggatatcaat cagccaggac cacagttgtt
     8461 tattctggaa gatgtgaccg gcgcaggtaa aacagaagcg gcgcttatcc tggcgcacag
     8521 gttgatggct gcgggtaacg ctcaggggct gttttttggc ctgccaacaa tggcaacggc
     8581 caatgccatg tacgatcgac tggtcaaaac ctggcttgct ttctattcgc cagaggtgtt
     8641 ccccgctcca gcggagataa accggcgcaa tacggtgcgg ttttcccgga agtaaaccgg
     8701 acgcactgga tgcgatgatg ggtatcactt agaattcccc gcccctgcgg tagaactccc
     8761 agctcccatt ttcaaaccca tcaagacgcc ttcgccagct ccttcaccaa cggtagcatt
     8821 atccgcataa cgtcacggca gcgacgttct attcttccag gaagtgcctt atcaatatgt
     8881 tgttgattat ccagtcttac gtcatgccag ctatttcccg ccgggaaggc aggtgttttt
     8941 gcgcgttgct gataaccatc cttatttcca agattccagt tagtcgcttc caccgaaagt
     9001 acagcaatgc ccgctttgtc gaatatttct gcgtcattac aacacccagt gcctttcgga
     9061 taatttttat tcaaacccgg attggtcgtt gcggctattc cgtgactgcg tgcaattgcc
     9121 agcgccctgt cgtgcgttaa tttccttact gcttcagggg tttttacacc gctgttgaaa
     9181 tacaatttat cgccaacaat taagttatcg agattaatca ccagcagcgt atttttcttt
     9241 tcggtgtcac tcatccgctt gagtaaattc tcagcgccta attttccttc ctcttcgccg
     9301 ctggtcgcca caaatcgaat accatactcg gtaggcgtat ttttcaggcg ttctgccagt
     9361 tccagcatga cacctaaacc tgcggcgtta tcatctattc cttgtaacgt cagtccgccg
     9421 agattggcat ctgcgtcagc atcgctcagc ggggcgtaag tatccagatg cgccataatg
     9481 atgatctgct gcggcgcttt gccttcatga gcggcaatca ccgtacttcc tgtcacgtta
     9541 tgccagttct tgcgattatc gcgggcggta taaatatacc gactattaaa tgtccgaata
     9601 tcactgcgat aacccatttg ctgaaactgt tggcgaatat aatcggcaga taacatttct
     9661 gctggagttc cggtcatgcg tcccggaaag aaagtagcaa tatgtcgtgc ctgggtatta
     9721 gcaaaatcgc caggtttagg tgacgaggcg tgtacgggga gaataaagca tacgccgagc
     9781 gccagggcag cggtacggtg gcgcaatgcg gaaaacatag tgagtcctta aataccatgc
     9841 aaattttttt accgccatag tatgaaactg ccgctgcgct aaaacaattt caaatcttcc
     9901 taaacgcccg aaatccggtg ccttaagcac tttttgatat tagctttgcc aaatcgttat
     9961 tccgttaagg aactactcat tctaattggt aatttcattc gttctcttac gctccctata
    10021 gtcgaaacat ctgatggcaa gaaaatagcg gtattgcaaa ggaacggtta tggatcaaaa
    10081 acgacttact cacctgcggc aactggaggc ggaaagcatc cacattattc gcgaggtggc
    10141 ggcagaattc tcaaatccgg tgatgctcta ctctatcggt aaagattcca gcgtcacgct
    10201 gcatctggcg cgcaaggcgt tttatccagg tacgctgcct ttcccgttgc tgcatgtcga
    10261 taccggctgg aaattccgcg agatgtatga gttccgcgat cgtacggcga aagcctacgg
    10321 ctgcgagctg ctggtgcata aaaacccgga aggcgtggcg atggggatta atccattcgt
    10381 gcacggcagc gcgaaacata ccgatattat gaaaactgaa ggcctgaaac aggcgctgaa
    10441 caaatacggt tttgatgccg ccttcggtgg tgcgcgccgt gacgaagaga aatcccgcgc
    10501 taaagagcga atctactctt tccgtgaccg cttccatcgc tgggatccga aaaatcagcg
    10561 cccggaactg tggcacaact acaacgggca aattaacaaa ggcgaaagca tccgcgtctt
    10621 cccgctctct aactggaccg agcaggatat ctggcaatac atctggctgg aaaatatcga
    10681 cattgtgccg ctgtatctcg ctgcggaacg tccggttctg gaacgcgacg gtatgttgat
    10741 gatgattgat gacaaccgta tcgacctgca accgggcgaa gtgatcaaaa aacggatggt
    10801 gcgtttccgt acgctgggct gctggccgct gaccggtgcg gtggagtcaa atgcacaaac
    10861 actgccggaa atcatcgaag agatgctggt ttccaccacc agtgaacgtc agggtcgcgt
    10921 gattgaccgc gaccaggcgg gatccatgga gctgaaaaaa cgtcaggggt atttttaaga
    10981 tgaacaccgt acttgcacaa caaatcgcca atgaaggcgg cgtcgaagcc tggatgattg
    11041 cgcaacaaca taaaagcctg ctgcgttttc tgacctgtgg tagcgtcgat gacggcaaaa
    11101 gtaccctgat tggtcgtctg ctgcacgata cccgccaaat ctacgaagat cagctctcat
    11161 cgctgcataa cgacagtaag cgtcacggca cccagggcga aaagctggat ctggctctgc
    11221 tggtggacgg cctgcaagct gagcgcgaac agggcatcac tattgatgtg gcctaccgct
    11281 atttctctac cgagaagcgt aaatttatta tcgccgacac cccagggcac gagcagtaca
    11341 ctcgcaatat ggcgactggc gcatcgacat gtgaactggc gatcttactg atcgatgccc
    11401 gtaaaggcgt gctcgatcaa acccgtcgtc acagttttat ctccacactg ttggggatca
    11461 aacatctggt cgtggcgatc aacaaaatgg atctggtgga ttacagtgaa gagacgttca
    11521 cccgtattcg tgaagattat ctgacctttg ccgggcagct gccgggtaat ctggatatcc
    11581 gctttgtgcc gctctccgca ctggaaggtg acaacgtggc ttcgcaaagt gaaagtatgc
    11641 cgtggtacag cggtccgaca ctgctcgaag tgctggaaac cgtggagatc cagcgagtgg
    11701 tggatgccca gccaatgcgc ttcccggtgc agtacgttaa ccgcccaaat ctcgattttc
    11761 gtggctacgc cggaatgctg gcatccggtc gcgtggaagt cgggcaacgt gtaaaagtgc
    11821 tgccctctgg tgtggaatca aacgtcgcgc ggatcgtgac ttttgatggt gatcgcgaag
    11881 aagcctttgc cggagaagcg atcaccctgg tgctgacgga tgagatcgat atcagccgtg
    11941 gtgatctgct gctggcggca gacgaagcgt taccggcggt gcagagtgcg tcggtggatg
    12001 tggtatggat ggcggaacag ccgctttccc tgggccagag ttatgacatc aaaattgccg
    12061 gtaagaagac gcgtgctcgt gttgatggca ttcgttatca ggttgatatt aataacctta
    12121 cccaacgcga agttgaaaac ctgccgctga acggcatcgg cctggtggat ctcacttttg
    12181 acgagccact ggtgttagat cgctatcagc aaaatccggt taccggtggg ctgattttta
    12241 tcgatcgcct gagcaatgtg accgtgggtg ccggtatggt gcacgagcca gttagccagg
    12301 caacagctgc gtcatctgaa ttcagtgcat tcgaactgga attgaatgcc ctggttcgtc
    12361 gccactttcc gcactggggc gcgcgcgatt tgctggggga taaataatgg cgctgcatga
    12421 cgaaaacgtc gtctggcata gtcatccggt cactgtgcaa caacgcgaac tacaccacgg
    12481 tcatcgtggt gtagtgctgt ggtttaccgg cctctccggg tccggtaaat caacggtcgc
    12541 cggggcgctg gaggaggcgt tacataaact cggcgtcagt acgtatctgc tggatggcga
    12601 caatgttcgc cacggattat gcagcgatct cggttttagc gatgccgatc gtaaagagaa
    12661 tatccgtcgc gtcggtgaag tggcgaattt gatggttgaa gccggactgg tggtgctgac
    12721 cgcatttatc tcgccacacc gcgccgaacg ccagatggtt cgcgaacgcg taggagaagg
    12781 gcgctttatt gaagtgtttg tcgatacgcc gctggcgatt tgcgaagccc gcgatccaaa
    12841 aggcttatat aagaaagcgc gtgccggtga actgcgcaac tttacgggaa tagattccgt
    12901 ttacgaagcg cctgaatcgg cagaaattca tctcaatggt gaacaattag taacaaattt
    12961 ggtacagcaa ttattagatc tgttgagaca gaacgatatt atcagatcct gagacaccac
    13021 cgggtttcca tgcccggttc gtcagagtaa caggatcaaa tatgcgcaat agccataaca
    13081 ttacactaac aaataacgac agccttactg aggatgaaga aactacatgg tcactgcctg
    13141 gtgccgtggt cggttttatc tcctggttat ttgcgctggc gatgccgatg ttgatttatg
    13201 gctctaacac gctgttcttc tttatctaca cctggccttt ctttctggca ctgatgcctg
    13261 tcgcggtagt ggtggggatt gcgctgcatt cattgatgga cggaaagcta cgctacagta
    13321 ttgttttcac cctggtgaca gtaggcatta tgtttggcgc actgtttatg tggctactgg
    13381 gctaatttgt actttcttgt ctcctctgtt cataattcaa accgtaacta ataatgagat
    13441 tatgttctgc acgccctggg tatacgtaac aatggacaaa tgtggtacat ttgcccgcgt
    13501 tgtcgcggta tccccaacag aggatgtaga gtcgtcttcg gatgcatggg atgatgatgc
    13561 cgtttttcag ggggcaggat gggtaaacta acgctgctgt tgctggctat tctggtctgg
    13621 ctacagtatt cgctgtggtt cggtaagaac ggtatacatg actatacccg cgtcaatgat
    13681 gatgtggcgg cactgcaagc tacaaacgcg aaacttaaag cgcgaaacga tcaacttttt
    13741 gccgaaattg acgatctcaa tggcggccag gaggcgctcg aagagcgtgc gcgtaatgaa
    13801 ctcagcatga tcaggccggg cgaaactttt tatcgtctgg tgcctgacgc gtcgaagcgc
    13861 gcacagtctg cggggcaaaa caatcgataa atcagcccgg gaattaacat ggcaaccact
    13921 catttggatg tttgcgccgt ggttccggcg gccggatttg gccgtcgaat gcaaacggaa
    13981 tgtcctaagc aatatctctc aatcggtaat caaaccattc ttgaacactc ggtgcatgcg
    14041 ctgctggcgc atccccgggt gacacgtgtc gtcattgcca taagtcctgg cgatagccgt
    14101 tttgcacaac ttcctctggc gaatcatccg caaatcaccg ttgtagatgg cggtgatgag
    14161 cgtgccgatt ccgtgctggc aggtctgaaa gccgctggcg atgtgcagtg ggtattggtg
    14221 catgacgccg ctcgtccttg tttgcatcag gatgacctcg cgcgattgtt ggcgttgagc
    14281 gaaaacagcc gcacgggagg gatcctcgcc gcaccagtgc gcgatactat gaaacgtgcc
    14341 gaaccgggca aaaatgccat tgctcatacc gttgatcgca acggcttatg gcacgcgctg
    14401 acgccgcaat ttttccctcg tgagctgtta catgactgtc tgacgcgcgc tctaaatgaa
    14461 ggcgctacca ttaccgacga agcctcggcg ctggaatatt gcggatttca tccgcagttg
    14521 gtcgaaggcc gcgcggataa cattaaagtc acgcgcccgg aagatttggc actggccgag
    14581 ttttacctca cccgaaccat ccatcaggag aatacataat gcgaattgga cacggttttg
    14641 acgtacacgc ctttggcggt gaaggcccaa ttatcattgg tggcgtacgc attccttacg
    14701 aaaaaggatt gctggcgcat tctgatggcg acgtggcgct tcatgcgttg accgatgcat
    14761 tgcttggcgc ggcggcgctg ggggatatcg gcaaactgtt cccggatacc gatccggcat
    14821 ttaaaggtgc cgacagccgc gagctgctac gcgaagcctg gcgtcgtatt caggcgaagg
    14881 gttatacctt tggcaacgtc gatgtcacta tcatcgctca ggcaccgaag atgttgccgc
    14941 acattccaca aatgcgcgtg tttattgccg aagatctcgg ctgccatatg gatgatgtta
    15001 acgtgaaagc cactactacg gaaaaacttg gatttaccgg acgtggggaa gggattgcct
    15061 gtgaagcggt ggcgctactc attaaggcaa caaaatgatt gagtttgata atctcactta
    15121 cctccacggt aaaccgcaag gcaccgggct gctgaaagcc aatccggaag actttgtggt
    15181 ggtggaagat ttgggctttg agcctgatgg tgaaggtgag catattctgg ttagaatcct
    15241 caaaaacggc tgcaataccc gttttgtggc ggatgcactg gcgaaattcc tgaaaattca
    15301 ttcccgtgaa gtcagcttcg ctgggcaaaa agacaaacat gctgtaacgg aacagtggtt
    15361 atgtgctcgc gtgccgggca aggaaatgcc cgatctgagc gcctttcaac tggaaggctg
    15421 ccaggtgctg gagtatgcgc ggcacaagcg caagctgcgt ttaggggcgc tgaaaggtaa
    15481 cgcctttacc ctggttctgc gcgaagtgag caatcgcgat gacgttgaac aacgtctgat
    15541 cgatatttgc gtaaaaggtg taccgaacta cttcggtgcc caacgttttg ggattggcgg
    15601 tagcaacttg cagggcgcgc tgcgctgggc gcaaaccaat actccggtgc gcgatcgcaa
    15661 taaacggagt ttctggttgt cggcagcccg cagtgcgttg tttaatcaga ttgttgctga
    15721 gcgcctcaaa aaagcagacg ttaatcaagt tgttgacggc gatgcgctac aattagccgg
    15781 acgtggtagc tggtttgtag caaccaccga agaactggcg gaattacagc gtcgcgtcaa
    15841 cgataaagag ctgatgataa ccgccgcatt gccgggcagt ggcgaatggg gaactcagcg
    15901 tgaagcgctg gcattcgaac aagcagctgt cgccgcagaa actgaattac aagctttact
    15961 ggtgcgcgaa aaagttgaag ccgcgcgcag agcgatgctg ctgtatccgc aacaattaag
    16021 ctggaactgg tgggatgacg tcaccgtaga gatccgtttc tggcttccgg cgggtagttt
    16081 tgcaaccagc gttgtcaggg aacttatcaa cacaacaggt gattatgcgc atattgctga
    16141 gtaatgatga cggggtacat gcacccggta tacaaacgct ggcgaaagcc ttgcgtgagt
    16201 ttgctgacgt tcaggtggtc gcccccgatc gtaaccgcag cggcgcttca aattctctga
    16261 cactggaatc ctccctgcgc acgtttacct ttgaaaatgg tgatattgct gtgcaaatgg
    16321 gaaccccgac cgattgtgtc tatcttggcg tgaatgctct gatgcgtccg cgcccggaca
    16381 ttgttgtgtc cggaattaac gccgggccga atctggggga tgatgttatt tattccggta
    16441 cggtagccgc cgcgatggaa ggccgtcatt taggttttcc ggcgctggcc gtctcgcttg
    16501 acgggcataa acattacgac actgccgcgg cggtaacctg ttcaattttg cgcgcactgt
    16561 gtaaagagcc gctgcgcacc ggacgtattc ttaatattaa cgttccggat ttacccttgg
    16621 atcaaatcaa aggtattcgc gtgacgcgct gcggtacacg acatccggca gatcaggtga
    16681 tcccgcagca agatccgcgc ggcaatacgc tgtactggat tggcccgccg ggcggtaaat
    16741 gtgatgccgg tccggggacc gattttgctg cggtagatga gggctatgtc tccatcacgc
    16801 cgctgcatgt ggatttaact gcgcatagcg cgcaagatgt ggtttcagac tggttaaaca
    16861 gcgtgggagt tggcacgcaa tggtaagcag acgcgtacaa gcacttctgg atcaattacg
    16921 tgcgcaaggt attcaggatg agcaggtgct gaatgcactt gccgccgtgc cgcgtgaaaa
    16981 attcgttgat gaagcgtttg aacaaaaagc ctgggacaat atcgccttgc cgataggtca
    17041 ggggcagaca atttcgcagc cgtatatggt ggcgcgaatg accgaattac tcgagctgac
    17101 gccgcagtcg cgggtgctgg aaattggcac tggttcggga tatcaaacgg caatcctggc
    17161 gcatcttgtc cagcatgttt gctcggttga acggattaaa ggcttgcagt ggcaggcacg
    17221 tcgccgcctg aaaaatcttg atttacataa tgtttcaacc cgtcatggcg atggatggca
    17281 aggttggcag gcacgtgcgc cgtttgacgc tatcattgtt acggcggcac cgccggaaat
    17341 tccaactgcg ctaatgacgc agctggacga aggcgggatt ctcgtcttac ccgtagggga
    17401 ggagcaccag tatttgaaac gggtgcgccg tcggggaggc gaatttatta tcgataccgt
    17461 ggaggccgtg cgctttgtcc ctttagtgaa gggtgagctg gcttaaaacg tgaggaaata
    17521 cctggatttt tcctggttat tttgccgtag gtcagcgtat cgtgaacatc ttttccagtg
    17581 ttcagtaggg tgccttgcac ggtaattatg tcactggtta ttaaccaatt tttcctgggg
    17641 gataaatgag cgcgggaagc ccaaaattca ccgttcgccg cattgcggct ttgtcactgg
    17701 tttcgctatg gctggcaggc tgttctgaca cttcaaatcc accggcaccg gtcagctccg
    17761 ttaatggcaa tgcgcctgca aatactaatt ctggtatgtt gattacgccg ccgccgaaaa
    17821 tggggacgac gtctacagcg cagcaaccgc aaattcagcc ggtacagcag ccacaaattc
    17881 aggctactca acaaccgcaa atccagccag tgcagccagt agctcagcag ccggtacaga
    17941 tggaaaacgg acgcatcgtc tataaccgtc agtatgggaa cattccgaaa ggcagttata
    18001 gcggcagtac ctataccgtg aaaaaaggcg acacactttt ctatatcgcc tggattactg
    18061 gcaacgattt ccgtgacctt gctcagcgca acaatattca ggcaccatac gcgctgaacg
    18121 ttggtcagac cttgcaggtg ggtaatgctt ccggtacgcc aatcactggc ggaaatgcca
    18181 ttacccaggc cgacgcagca gagcaaggag ttgtgatcaa gcctgcacaa aattccaccg
    18241 ttgctgttgc gtcgcaaccg acaattacgt attctgagtc ttcgggtgaa cagagtgcta
    18301 acaaaatgtt gccgaacaac aagccaactg cgaccacggt cacagcgcct gtaacggtac
    18361 caacagcaag cacaaccgag ccgactgtca gcagtacatc aaccagtacg cctatctcca
    18421 cctggcgctg gccgactgag ggcaaagtga tcgaaacctt tggcgcttct gaggggggca
    18481 acaaggggat tgatatcgca ggcagcaaag gacaggcaat tatcgcgacc gcagatggcc
    18541 gcgttgttta tgctggtaac gcgctgcgcg gctacggtaa tctgattatc atcaaacata
    18601 atgatgatta cctgagtgcc tacgcccata acgacacaat gctggtccgg gaacaacaag
    18661 aagtgaaggc ggggcaaaaa atagcaacca tgggtagcac cggaaccagt tcaacacgct
    18721 tgcattttga aattcgttac aaggggaaat ccgtaaaccc gctgcgttat ttgccgcagc
    18781 gataaatcgg cggaaccagg cttttgcttg aatgttccgt caagggatca cgggtaggag
    18841 ccaccttatg agtcagaata cgctgaaagt tcatgattta aatgaagatg cggaatttga
    18901 tgagaacgga gttgaggttt ttgacgaaaa ggccttagta gaagaggaac ccagtgataa
    18961 cgatttggcc gaagaggaac tgttatcgca gggagccaca cagcgtgtgt tggacgcgac
    19021 tcagctttac cttggtgaga ttggttattc accactgtta acggccgaag aagaagttta
    19081 ttttgcgcgt cgcgcactgc gtggagatgt cgcctctcgc cgccggatga tcgagagtaa
    19141 cttgcgtctg gtggtaaaaa ttgcccgccg ttatggcaat cgtggtctgg cgttgctgga
    19201 ccttatcgaa gagggcaact tggggctgat ccgcgcggta gagaagtttg acccggaacg
    19261 tggtttccgc ttctcaacat acgcaacctg gtggattcgc cagacgattg aacgggcgat
    19321 tatgaaccaa acccgtacta ttcgtttgcc gattcacatc gtaaaggagc tgaacgttta
    19381 cctgcgaacc gcacgtgagt tgtcccataa gctggaccat gaaccaagtg cggaagagat
    19441 cgcagagcaa ctggataagc cagttgatga cgtcagctgt atgcttcgtc ttaacgagcg
    19501 cattacctcg gtagacaccc cgctgggtgg tgattccgaa aaagcgttgc tggacatcct
    19561 ggccgatgaa aaagagaacg gtccggaaga taccacgcaa gatgacgata tgaagcagag
    19621 catcgtcaaa tggctgttcg agctgaacgc caaacagcgt gaagtactgg cacgtcgatt
    19681 cggtttgctg gggtacgaag cggcaacact ggaagatgta ggtcgtgaaa ttggcctcac
    19741 ccgtgaacgt gttcgccaga ttcaggttga aggcctgcgc cgtttgcgcg aaatcctgca
    19801 aacgcagggg ctgaatatcg aagcgctgtt ccgcgagtaa gtaagcatct gtcagaaagg
    19861 ccagtctcaa gcgaggctgg cctttttctt ttgtttggga agcttgtgtt tttgtcgcct
    19921 ggataagaca cgtcagcgtc ctacccggca atcagcattc tatgtgctca tcatcttgcg
    19981 caccaactgc ataaacagct cctgctcttc tccactcaaa cgccccaaaa attcctcatc
    20041 cacgctgtca ccaataggta tcgccgcagc aagtagcttt tctccttcgg cagtcagcca
    20101 gacaaagcgt cgccgcttgt cagcgacgtc atgttcacgt ctgactaagc cacgattttc
    20161 cattcttgcc aacatttctg ctagcgttgc tttggtgctg actgctgctt ctatcagtgc
    20221 cacctgttca ataccaggtt tatcagcaat ggcgcgcatc accgcgtact gtggtttggt
    20281 caggtcaggt aactcgtgct gccagcgagc cgtgtgctgc tgaaaaagct gtcgtaactg
    20341 atggaacgct ttatttcgta acgccatgta aactccgggc taattctctg tcgctatcat
    20401 agcggttttt tagtgacgaa gaagatgttg ttacttttca atccttgccg ctgtggaaat
    20461 gaaggagtac gttaataata acgttcgtat acgaacaatt aagagaataa gcaatgaaac
    20521 tgatcgtcgg gatgacaggg gctaccggtg cgcctcttgg tgtggcatta ctgcaagcgc
    20581 tgcgtgagat gccgaatgtc gagactcatc tggtgatgtc gaagtgggcg aaaaccacca
    20641 ttgaactgga aacgccttac agcgctcgcg atgttgctgc cctcgcagac ttcagccata
    20701 acccggcgga tcaggcggcg atcatctcat ccggttcttt tcgtaccgac ggcatgatcg
    20761 ttattccgtg cagtatgaaa acgctcgccg gtatccgcgc tggttacgcc gatggcctgg
    20821 tagggcgcgc ggcggacgtc gtgctcaaag aaggccgcaa actggtgctg gtgccgcgtg
    20881 aaatgccgct tagcaccatc catctcgaaa atatgctcgc actttcacgc atgggcgtgg
    20941 cgatggtgcc gccgatgcct gccttttata accatcccga aacggtagat gacattgtcc
    21001 accatgtggt agcccgcgtg ctggatcaat ttggcctcga acatccccac gccaggcgct
    21061 ggcaaggatt gccgcaggcc cggaattttt ctcaggagaa tgaataatgg catttgatga
    21121 tttacgcagc tttttacagg cgcttgatga ccacggccag ttactgaaaa tcagcgaaga
    21181 agtgaacgct gagccggatc tggcagcagc agctaacgcc accgggcgta tcggcgacgg
    21241 cgcgcccgcg ctgtggtttg ataatattcg cggctttacc gatgcccgcg tggcgatgaa
    21301 taccatcggt tcctggcaga accacgcgat ttcccttggc ctgccaccaa acaccccggt
    21361 caaaaagcag attgatgagt ttatccgccg ctgggataac ttcccgattg ccccggagcg
    21421 ccgcgccaat ccagcctggg cgcagaacac cgtcgatggc gaagagatca acctgttcga
    21481 tattctgccg ctgtttcgcc tgaatgatgg cgatggcggt ttctatctcg acaaagcgtg
    21541 cgtggtttcc cgcgatccgc tcgacccgga taacttcggc aagcagaacg tcggcatcta
    21601 ccgcatggaa gtgaagggca agcgtaagct cggcctgcaa ccggtgccga tgcacgatat
    21661 cgccctgcat ctgcataaag cagaagagcg cggtgaagat ctgccgattg cgatcacgct
    21721 cggtaacgat ccgatcatca cgctgatggg ggccacgccg ctgaaatacg atcagtctga
    21781 gtatgaaatg gcaggcgcgc tgcgtgaaag cccgtacccg atcgccaccg ccccgctgac
    21841 cggttttgat gtgccgtggg gttcagaagt gatcctcgaa ggggttatcg aaagccgtaa
    21901 acgcgaaatc gaagggccgt tcggtgagtt taccgggcac tactccggtg ggcgcaacat
    21961 gaccgtggtg cgcatcgata aagtctctta tcgcagcaaa ccgattttcg aatcactcta
    22021 tctcggtatg ccgtggaccg aaatcgacta cctgatgggg ccagccactt gcgtaccgct
    22081 gtatcaacaa ctgaaagccg agttcccgga agtgcaggcg gtaaatgcca tgtacaccca
    22141 cggcctgctg gcgattatct ccaccaaaaa acgctacggc ggctttgccc gcgcggtggg
    22201 cctgcgtgcg atgaccacgc cgcacggtct gggctacgta aagatggtga ttatggtcga
    22261 tgaagacgtt gacccgttca acctgccgca ggtgatgtgg gcgctctcct cgaaagtaaa
    22321 cccggcaggg gatttggtgc agttgccgaa tatgtccgtg ctggaactcg accctggctc
    22381 aagcccggcg gggatcaccg acaagctgat tatcgacgct accacgcctg tcgccccgga
    22441 caaccgtggt cactacagcc agccggtggt ggatttgccg gaaaccaaag cctgggctga
    22501 aaaactgacc actatgctgg ctgcacgtaa ataaggagaa caagatgatt tgtccacgtt
    22561 gtgccgataa acagattgaa gtgatggtga aatcgccggt gaaagatgtc tggacggtat
    22621 atcagtgcca gcattgcctt tatacctggc gcgataccga accgctacgc cgcaccagtc
    22681 gcgaacatta cccggaagcg ttccgcatga cgcagaaaga tattgatgat gcgccaatgg
    22741 tgccgagcat cccgccgctg ctggcggaag gtaagcgcta aataaaaagg tccgatgccc
    22801 atcggacctt tttattaagg tcaaattacc gcccatacgc accaggtaat taagaatccg
    22861 gtaaaaccga gaatggtcgt taacactgtc caggttttca gaccgtctgc taccgacaac
    22921 cccagatatt tggtcacaat ccagaaccct gagtcattaa tatgtgacgc cccaagccca
    22981 ccaaagcagg ctgccagcgt caccaatacg cactgaatcg gattcaatcc catcaccgct
    23041 tctgagagta acccgccggt tgtcagtatt gctacggttg ctgacccctg cgatgcacgc
    23101 agcgccagtg aaataataaa tgcggctggt aacagaggca ggtcaatcat ttgtaacatg
    23161 ttggcaaggg ctttgccgac gcccgattcc accagcactt tgccaaatac ccctccagca
    23221 ccagtaacca aaatcactac cgccgcagta ggaagcgctg agcccataat gtcgccggtg
    23281 tgttgtaagc tccagccgcg acgtaaagcc aataaccaga atgccagcac cagcgcaatc
    23341 attagagcta ccattggtga gccgatcagc tgtagcgtac caagcagggg atgcgaaggc
    23401 ggcatcagtg ttgcggaaac cgtacccgcc atgataatcg cgataggaat aacaattagc
    23461 gaggtgacca gcgcgacgcc cggtggattt attttatcgc ttaattttgt cgcgccttcc
    23521 tcactggccg gagccagttg catctgttcc agtacttcta ctgacatcgc atattggcgt
    23581 ttattgatta ttttcgctgc aaagtagcca acaaccccta cgggaataga aatcgcaata
    23641 ccgatgatgg ttagccagcc gatgtctgcg tggagtaacc ccgctgcggc gacagggcct
    23701 ggatgcggcg gtaccgccac gtgaacagtg agcatgatcc cagcgacagg caggccaaat
    23761 ttgagtggcg atattttggc taccttggca aaaccgtaaa tgattggcgc aagaataata
    23821 aagccgacat caaagaagac gggaataccg aggaagaacg ctgccagagt cagcgcagcg
    23881 atagttcgtt tgtcacctaa cttgcgactg aaataattag ccagtgactc tgcaccacaa
    23941 gagtgttcga tcatacgccc cagcatagcg cccagaccaa taataatagt gacggaacca
    24001 agcacaccgc ccatcccggc gatcatcact ttacccactt cgcccgccgg tatacccgcc
    24061 gcaagtgcga ctaacaggct gacgaggagc agagcaacga atggttgtac ctttgccttg
    24121 atgaccagca gcaacagcat gattacgcca gctaacgcaa tgcataacaa tgtaattgtg
    24181 gacatgggaa accctgtctg aaagttatag ttaacctacc ccatccgtag atggggggat
    24241 gtatgggtac gttgtaatta gggatttaac gaattagcgc taggcgtcaa accagccaag
    24301 cccttcttcg gtgaggccac gaggtttata ttcacaaccg atccagccct gatatcccac
    24361 ctcatcgaac aggcggaaca gccacggata gttgatttct ccatcgtccg gttcatgtcg
    24421 atcaggtagt ccggcaattt gtacgtgcgc atatttcccg gcgtagtcgc ggattaaatg
    24481 cgtcaggttg ccatctactt tttgcgcatg aaaagtatct agttgaataa acacgttatc
    24541 tcgcgcaacc tcttcaacaa tagccagtgc ctgatactgg ctggagaaga gataatgagg
    24601 cttaacgccg gggctgagtg cttcaactaa tattcgcttg ccgtgtggcg caaagcggtc
    24661 ggcagcgtag cggagattat cgataaatac tgcccggtac cgttcagcat cttcgccagc
    24721 gggcacgacg cctgccatca catggacttg ttcacaattg agcgccaatg catattccag
    24781 tgccaggtcg atgtctgcgt gtgcttcgtg ctcacgtccg ggaagggcgg ataatcccca
    24841 ttcccccgca ttaatatctc cgggagcggt attgaacagc gccagtgtca gatggttttg
    24901 ctccagttgc ttttggattt gcagggtgga gtagttatag ggaaacagaa attccacagc
    24961 atcgaacccg gcttttcgcg ctgcggcgaa gcgttcaata aaaggcactt cggtgaacat
    25021 catggataaa ttagctgcaa aacgaggcat tgcattaact ccttaattcc gcaatttcac
    25081 ctgcggtcag ataacggatc gggcggtcac cgagaataaa aatcagcttt gccgtttcct
    25141 ccagctcttc catattgttg gcggcttctt gcaggctttc accgcaaacc actgggccat
    25201 gatttgccag taaaaaagcc tgattgtctg ctgccagttc cgccagatcc tgtgcgatgc
    25261 gtttatcgcc cggtcggtaa tagggcacca gcgggacatt tcccatccgc atcaccacgt
    25321 atggtgtgaa cggacgaata acgttgctgc tgtccagccc ttgcaggcag gaaagcgccg
    25381 tcgaccatgt gctgtgcaaa tgcaccaccg ctttacagcg cggattgttg cgatacagcg
    25441 ccagatgaaa gagcacctct ttcgagggtt tgtcaccact taaccattcg ccatccgcgg
    25501 cgactttgga aagccgctgc ggatcgagat tgcccaggca tgaacctgtc ggtgtcgcca
    25561 gtaaattccc gtcaggtaaa agcagcgaca gattgccagc cgaaccggtt gcatagccgc
    25621 gctgaaagaa tgaactggca atccgcgtca tctcctctcg caaagactgc tctacttttg
    25681 cgaaatcgct catgataaaa actctctttg ggctcgtgaa aaaaaggctt catcaccgaa
    25741 gttgccagat ttaagggcga gtgagacagg cttatccagt gcgtttaccc acggcacgcc
    25801 gggggaaatg gttgggccaa tatgaaaccc ttttatgccc aggctctgtg tgactacgcc
    25861 ggaggtctca ccgcctgcga caataaagcg tgtcacgcct tccgctgcta accgcgccgc
    25921 tagttgagaa aacagagttt ctactgcctg actggctttt tgtgcaccgt attgctgttg
    25981 aattgctgcc aatgcgtcag tgctggcggt ggcaaaaacc agtggagcaa gtacactttc
    26041 ctggcccaga acccactctg ccagttcgtg tgcataagcg gccagagttt cggttgagag
    26101 gcagtgtgcc acatcaactt cacgggctgg tgcaatttga cggtaatgtg ccacctggcg
    26161 gttggtcatt tgagagcatg aaccggagag cactacgccg cgcccagcga gcggacgccc
    26221 tgcttcgcga gcctggttac cgttttcttg cgcccactgc cgggccaggc caatcgccag
    26281 accagaaccg cccgttacca gtggggcatc gcgcaaggct tctccctgaa tttccagatg
    26341 gtgttcggtc agcgcatcaa gcaccgcgta gcggtagccc tcttgctgta agcgagccag
    26401 ctcttgacga acggcatcca caccttgttc gaaaacatgt gccgaaacga cgccgcagcg
    26461 ccctgtggat tgcgcttcaa ccagacgggg aagatagctg tcggtcatgg gatttaccgg
    26521 gtgatggtgc atcccggatt cggccagcag ttgattcatt acgaacaaat acccctgata
    26581 aaccgtacgt ccgttgaccg gcagggccgg agagaagacc gtaaacggcg tgtcgagagc
    26641 atccattaat gcatcggtaa ccgggccaat attacctttc gccgtactgt cgaaagtaga
    26701 gcagtatttg aaatagatct gtttgcaacc ttgctgttgc aaccagctca gagccgccag
    26761 cgattgctgt gtggcttcaa ccaccggaca ggagcgcgtt ttcaggctga tcaccagtgc
    26821 gtcgattgct tccggcattt tacctgttgg aacaccgtta atttgtaccg ttggtagacc
    26881 gttttccacc agaaaactgg cgatatccgt cgcgccggta aaatcatcgg cgataacgcc
    26941 aatcttgatc atgatttcgc tcccggtaga gtgatgccag agaaaatctt gataactgcg
    27001 ctatcgtctt ctttcccgta acccgcgtta ctggcgctgg tgaacatatt caatgctgtt
    27061 gaggccaatg gcagcgggaa gtgcagggct ttggctgtat cggtaaccag accaagatcc
    27121 ttaacaaaaa tatcgacggc tgaatgcggg gtgtaatcgc catccaccac atgacgcatc
    27181 cggttttcga acatccagga atttccggcg gcattggtca cgacgtcata catcacatcc
    27241 agcgggatcc ccgcacgggt gccagtcgtt caaaggcaat atcgctaccg gaggccatga
    27301 cagtcatttc accgttagcg gcttttactg caccaccaga aactggcgca tccagcattt
    27361 ccagatcgaa tccagccaga gcggtagcaa tttcttgcgc atcagcacta gcgatagtgg
    27421 aagaaaccat tactgccgta ccgggtttca gatgttgtgc aacgcctgtt tcaccaaaca
    27481 gcacctgttt aacctgggcc gcattgacca ccagcaccag cagagcgtcc agtttttcgg
    27541 caaacgtcgc ggcgttatca gaaaccccgc aagcacctgc ctctttcaac gtagcgcagg
    27601 cattgctgtt caggtctgcg ccccaggtag aaaaacctgc gcggacacat gacagtgctg
    27661 ctcccattcc cattgaccct aagccaacga taccgacatg aaactcagat cccgttttca
    27721 tctgctctta ttgttaattt aagtgatatt ttgtttgata ttgtgaatat gagcgctgaa
    27781 agataacgat atggtgagct gattcacata aattaacatt ttgtgttatt ttatgtgaac
    27841 taagcgttag ttgcgccgcg cacgtttcgc aggcaaatag cgtagaatgt cagcaggaca
    27901 aaggaaggag caaaagttga tacccgtaga gcgtcgccaa atcatccttg agatggtagc
    27961 tgaaaaaggc attgtcagta ttgctgaact aacggacaga attaatgtgt cacatatgac
    28021 cattcgtcgg gatttacaaa aactggagca gcagggagcc gttgtgctgg tgtccggagg
    28081 cgtccagtct ccgggacgcg tggcgcatga accttctcat caggtaaaaa ctgcgctggc
    28141 aatgacgcaa aaagcggcta ttggcaagct ggcggcaagt cttgttcagc cgggaagttg
    28201 tatctatctg gatgcgggaa cgaccacgtt agcgatagca cagcatctga ttcacatgga
    28261 gtcactgact gtggtcacaa acgatttcgt tattgcgaac tacttgctcg acaacagtaa
    28321 ttgcacaatt attcacactg gcggtgcagt gtgtcgggaa aaccgttcct gtgtcgggga
    28381 agccgctgcg accatgctgc gcagcctgat gattgatcag gcttttattt ctgcatcgtc
    28441 atggagtgtg cgggggattt ctacgccagc ggaagataaa gtcacggtga aacgggcgat
    28501 tgccagtgcc agccgccagc gagttttggt ctgtgatgcg acgaaatatg gtcaggtggc
    28561 gacatggctg gcgttaccat taagcgagtt tgatcagatt attacagacg acggtttgcc
    28621 ggagagtgcc agtcgcgcgc tggcgaagca ggatctctct ttgctggtag cgaaaaatga
    28681 ataatggcct gcaataacat ctggttactc atgcttcaca gaagaagcat gagactacct
    28741 tattttataa aatgacagcc gcccactttt cggcgatccg gtatcaatat aaatctggtt
    28801 agcgaacgtc tgaatgttat caaacatcat atgtccaaat ataaaataat cagcgccgtt
    28861 tatttgttgt aactcgccat taagcgattt ctgcatacga taaacaggac agagtaattc
    28921 gctctccgct atttctttgc caaagagata ttcatcaccc ggataatctg catgtgcgat
    28981 gacatatttt attatgtcgt tagtgatttc aataatatgt ggaaggtgat ggaatttcag
    29041 caacagatct attgcctctt gttgctctga atcatttaaa tcgaaaaacc agtcaccacc
    29101 gctggcaagc cacatattgc catcgccggt cgcgaacgca tccagcgcca ttgcttcgtg
    29161 gttgccttta acggagataa accagggctg gtttagcagg cgcaggacgt caagactctc
    29221 cggcccacga tcaatattat cgccgacaga aataagtaag tcggtttcgg ggaaaaaaga
    29281 gagttgatgt aagcgggatt gtaataactg atattcacca tgaatatcac caacgaccca
    29341 tatatggcga tagtgatggg cattgatttt ttgatagcgt gtagatggca tggttttacc
    29401 ctgtaaaata agctttccta ttatacaggg tatttttatt tgattcgtca gttgtcgtta
    29461 atattcccga tagcaaaaga ctatcgggaa ttgttattac accagactct tcaagcgata
    29521 aatccactcc agcgcctgac gcggagtcag tgaatccggg tcgagatttt ccagtgcttc
    29581 gaccgcaggc gaagtttctt ctggcactga cagcaaagac atctgcgtac catccacttg
    29641 cgtcgcggcg gcgttcggcg aaatgctttc cagctcacgc agtttttgcc gtgcgcgctt
    29701 aataacctct ttcggtacgc ccgccagagc agcaaccgcc agaccgtagc ttttgctcgc
    29761 cgcgccgtcc tgcacgctgt gcataaaggc gatagtgtcg ccgtgctcca gcgcatcgag
    29821 atgtacgttg gcgacgcctt ccattttctc cggtagctgg gtcagctcga aatagtgggt
    29881 ggcaaacagc gtcaatgcct taatcttatt cgccagattt tctgcgcacg cccacgccag
    29941 cgacagacca tcgtaggtgg acgttccacg cccgatctca tccattaaca ccagactgta
    30001 ttcggtggcg ttatgtaaaa tattggcggt ttcagtcatc tccaccataa aggttgagcg
    30061 cccggaagcc aggtcatctg ccgcgcctac gcgggtaaag atgcgatcga taggtccaat
    30121 ctcgactttt tgtgccggta catagctgcc gatgtaggcc atcagcgcaa tcagtgcggt
    30181 ctggcgcata taggtacttt taccgcccat gttcgggccg gtaataatca acatacggcg
    30241 ctgcggcgac agattcagcg ggttagcgat aaatggccca ttcagtactt gttcaactac
    30301 cggatggcga ccttcggtaa tgcgaatgcc cggtttatca atgaaggtcg ggcaggtgta
    30361 gttcagggta taggcccgtt ccgccaggtt aaccagcacg tcgagttccg ccagcgcgct
    30421 cgcgctctgt tgcaacgctt ccagatgcgg caacagcagg tcgaacagct cttcataaag
    30481 ctgtttttcc agtgccagtg ctttgccttt tgaggtgaga actttatctt cgtactcttt
    30541 tagctctgga atgatgtagc gctcggcgtt tttcagcgtc tggcgacgca tgtagttgat
    30601 gggtgccaga tggctttgcc cacggctgat ttgaatgtag tagccgtgca ccgcattaaa
    30661 gccaactttc agcgtgtcca ggccggtacg ttcacgctcg cggacttcca gacgctccag
    30721 ataatcggtc gcgccgtcag ccagcgcgcg ccactcatcc agctcttcgt tatagcccga
    30781 tgcgataaca ccaccgtcgc gtaccagcac cggcggtgtg tcgatgattg ctcgctccag
    30841 cagatcgcgc agctcggcaa actcgcccat cttctcacgt agcgcctgta ccggtgcact
    30901 atcgacagtt tctaactgcg cacgcagctc cggcagttgc tggaaagcgt ggcgcatacg
    30961 ggccagatcg cgtgggcgag cagttcgtaa agccagacgt gccagaatac gttccaggtc
    31021 gccgacctga cgcagtaccg gctgcaactc ggcggtgaaa tcctgcaatg cgccaatagt
    31081 ttgctggcgc tcaagcaaca cgcgggtatc gcgcactggc atatgcagcc agcgtttcag
    31141 catacgacta cccatcggcg tgacggtgca gtcgagcaca gaagccagcg tattttccgc
    31201 accgcccgcc aggttctgag taatttccag gttacgacgt gtcgcggcat ccataatgat
    31261 gctgtcctgc tcacgttcca tggtgataga acgaatatgc ggcagggtcg tgcgttgggt
    31321 atctttcgca tactgcaaca gacaaccggc agcacaaagt ccgcgcggcg cgttctcgac
    31381 gccaaaaccg accagatcgc gggtgccaaa ttgcagattc aactgctggc gcgcggtgtc
    31441 gatttcaaac tcccacagcg ggcgacggcg caggccgcga cggccttcaa tcagcgacat
    31501 ctcggcgaaa tcttctgcat acagcagttc cgccggatta gtgcgttgca gctctgccgc
    31561 catcgtttcg cggtcggccg gttcgctcag gcgaaaacgc ccggagctga tatccagcgt
    31621 cgcgtagccg aaacctttgc tgtcctgcca gatagccgcc agcaggttgt cctgacgctc
    31681 ctgtaacagg gcttcatcgc tgatggtgcc tggcgtaacg atacgcacaa ctttgcgctc
    31741 aaccggacct ttgctggtcg ccggatcgcc aatttgttcg cagatggcaa cggactctcc
    31801 ctgattcacc agtttggcga gatagttttc caccgcatgg tagggaatcc ccgccatcgg
    31861 gatcggctct cccgccgaag caccgcgttt ggtcagtgaa atatccagca gttgcgacgc
    31921 gcgttttgcg tcgtcataaa acagttcata aaaatcaccc atccggtaaa acagcaggat
    31981 ctcgggatgc tgggctttca gcttgagata ctgctgcatc atgggcgtat gggcgtcgaa
    32041 attttctatt gcactcatgg ggttatgtcc ggttccctga tattaaatgg ggtgtgatgg
    32101 tttttatttt cgttgtaatc acataaggcg cacatgatac cggagttaat catttcaggg
    32161 gaagaagcga acggcgagtt cttgatgctg gttccgggaa agatgctgtt ttgcagcttt
    32221 tgcgtgatgc ttatgaagca agtacgcaaa attgtatcga ttggcgaacc cttcttcttt
    32281 agagtcatag tgccctttaa gattaagatg taatataaat gcatcttaat gaggtggagg
    32341 cagcatgtcc ggcaagcgta tctctcgtga aaaactgacg attaaaaaaa tgatcgatct
    32401 ttatcaagcg aaatgcccgc aggcgtcagc ggagccggag cattacgagg cgttgtttgt
    32461 ttacgcgcaa aagcggctgg ataaatgtgt gttcggcgaa gagaaaccag cctgtaagca
    32521 gtgtccggtg cactgttacc agcctgcaaa gcgtgaagag atgaaacaga ttatgcgttg
    32581 ggccgggccg agaatgctgt ggcgtcatcc gatcttaacc gtgcgtcatc tcatcgatga
    32641 caagcgtccg gtgccagaac tgccggaaaa atatcgtccg aaaaaacctc gtgaatagta
    32701 tgccggacag gcagatctgt ccggcaattt gcagttaaat caatgccgat ttatcaattc
    32761 ccagccgctt catccgtgac agcagggtcg tgcgtttcag ccccagacgt tgcgcagcgc
    32821 ctttaggccc ggcaaccacg ctgttggttt ctttcagcac gcgcacaatc aactgatatt
    32881 catcttcgcc ctcctgggcg acaaccgttg cggcaggcgg cgtttcaggc tccggtaaag
    32941 caatatctgg caatgacagc tgcagcacgt tgccgcgtgt tagcaatact gcgcgctcaa
    33001 tgacgttttc cagttcgcgt acgttacccg gccactccat gttgctcaag gtgcgcagcg
    33061 tctcggcagg aatgctgtcg atattgcgcc ccagacgacg ggcaattttg aaggtaaagg
    33121 ctttcgccag cagcggaata tcttccggac gctcgcgtag tggcggcagg tgaatcggga
    33181 atacgttcag gcggtaatag agatcgctac ggaactctcg gtcggcgacc atttttttca
    33241 gatcgcggtt agtcgcggca attaaacgta cgtccgtctg aatgattttg ttgctgccga
    33301 gacgttcaaa ctcctgttcc tgcaatacac gcagcaactt cggctgtaac tccagtggca
    33361 tatcgcccac ttcgtcgagg aacagagagc ttttatccgc cagttcaaaa cgaccaatgc
    33421 gctgggcgct ggcaccggta aaagccccac gctcatgacc aaacagatcg ctttccagca
    33481 atccggcagg catcgccgcg cagttcattt tgaccatgcg gcgattatta cgcccactga
    33541 gattatggat cgcacgggca atcagctctt taccagtgcc agtttcaccg aggatcagca
    33601 cggtactgtc actttgcgcc accatttcaa cttgtttaag cacgctgtac atggcttcgc
    33661 tgcggccaat aatctcgcca aattcactat caacattgtt gagctgctcg gtcagggcga
    33721 ggttttcatc aaccagccgt tctttcagac gatggatttc ctgataggcg agggcgttat
    33781 cgacagcgat tgccacacgt tcggcaatct ggcgcagcaa attcagattg gtagtggtaa
    33841 acactttctc ttcgcattgc gccagtttca gcacgcccag catggtgtcg ccagacatca
    33901 gcggtaacag gcacaaggtt tgaatctggt tgccccaggt gtcgaacaac atgcgttcat
    33961 agggggctaa atcgtcccgc tcgtggagat tgatcagcag catctcttta cttttgaaca
    34021 cgcgttcggt gagggttccg gcttcatcga cttcgctctg ttcgtgggcg ggatgttgtt
    34081 tatcaagata gtgagtggag tagatgttga gtttgttttt acggtggctg cgtaagacaa
    34141 tgctgatatc gtcaatgtcg aaatagtaat ggatttcttt ggcgacttcg ctgaccagtt
    34201 cgtccatatc caggcgggaa agcaccgcgt tggtaatggc aaccaggatg cggaagttat
    34261 cgagctcccg gcataacaac tcatagtcga cattgttgac gacgcggctc tggatttgtt
    34321 cggtgacgac agaaacgatc tgcgtaaatg tttgcagacg attgaactct ttttcgctcc
    34381 agggacgatc gtcataacga ataaattcac agccaccaaa aatatgccct tccgccgcca
    34441 gtggcatcag gcaatagtga ccaaatttgg ggtatagccc acctgcggcc aggtttcaca
    34501 aaattcttcg taactgcaat gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc
    34561 gtgtgccaga acagtttcgt cttcatattt aatgggggtg tctttttcac gcgacgcgta
    34621 ataagacgca cgttgagtct gcgcttgcca caacacaata gccgcgttgt cggcgagcgc
    34681 agaacgcttt accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa
    34741 tagtgtccga gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga
    34801 cattttgttc ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag
    34861 gagatttaga attttattag catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg
    34921 ttcgtttcac gccatacaga ccggcaagac gaacaccttt acgttccacc acttcaccaa
    34981 tcagcgccgc gtctttcccc agtggatggg aatgtaacgc tgccagcact tgctctgccg
    35041 cgttgcgttc aacggcgatc accagtttgc cttcgttggc aaagttcagg gcgtccagtc
    35101 ccagcaattc gcaaacgccg cgcacggcag gtttaaccgg cagcgctgat tcagaaattt
    35161 caataccaca accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg
    35221 tggcatcacg cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca
    35281 gcaccgcgca gtcgctgacc agttcgccat ccagccccag ctgctcacgc aggttaagga
    35341 tagtcgcccc gtggtcgccg agtgtaccgc taaccagcaa tacatcgcct gcggttagcg
    35401 tctgtgcgcc ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca
    35461 gtttatctgc cgcgccgcgc tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg
    35521 ccgtgcgggc ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc
    35581 cttcttcgag gataaagcca caggagagat agcgcggaat agcgccactg accgcaacgt
    35641 cattcgcggt gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt
    35701 caatgacgta actgtcggtg gagaacgcca gacggtcgcc ttccgctacc agctgcgcca
    35761 gatcaagacg tgcctgatct tcctgctctg ccagccacgg gttggcaaag gcttccataa
    35821 acaggctgtt gattaattgc tgcatcgcct ggccgccgct gccgtgggcg agttggatat
    35881 tattcacgct tcactctcct gctgacgata ctgataccac gcggcgcacg ctccttcgga
    35941 ggagaccatc agtgcaccaa atgcggtttg aggattacag gtgttaccaa acagcgggca
    36001 ttgatgcggc ttacatttgc ccgttaatac ctcaccacaa cgcgcgcgcg ggtcatcgca
    36061 gacctgctgc ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt
    36121 caggtgcacg ccagaagatt caatcacgcc taagccgcgc cattcgctgt cgccgttgac
    36181 acagaacaca tcggcaatcg cctgttgcgc cagcaggtta ccggcatccg gtaccacccg
    36241 acgatactga ttctctacct tgctgtgggc cgctattttc tgctccacca gcatgaccac
    36301 gccttgcagt agatcaaggg gttcgaaacc agcgaccacc agcggacgat gaaaatcgct
    36361 ggcgataaaa ttataggcat cagtgccgat aaccatacta acgtggcccg gcgcgaggaa
    36421 cgcgtcgata ccgttatccg gctgttccag caaactgcgc agcgtcggga taagcgtaat
    36481 gtgctggcag aagaagtaaa aattctgcac atcacgcgct ttcgcctgtt gcagagtgat
    36541 ggcggtggtc ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg
    36601 attctcctgc gccagtttca acgcatccat cggcgagtaa acgatgcgca catcggcacc
    36661 gcgtgctttt gcctgcaaca gcgatccctg tttccctggt acgcgcatgg cgtcgccaaa
    36721 ggtacagaag atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat
    36781 cggcagtacg cacaccgggc aacccggacc gtggataaac tcaacgtttt caggcagtaa
    36841 ctggtcgagg ccgaatttaa aaatggcgtg ggtatgaccg ccacacactt ccataatccg
    36901 cagagggcgt tcggcggtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa
    36961 ctgcatcacc tgttccggtg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca
    37021 tacaacagcg cgccgacatc cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg
    37081 tcgcgtgctt cggcttcatt aattacgctc atggcaaagc caacgtgaac cagtacccac
    37141 tggcccacgc gcggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg
    37201 cgctgaatgc cgcagacgtc gactttcgcc tggttgccgt caatggtgcg gatctggccg
    37261 ggaacgccta tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcgc
    37321 cgctggtggc ggaaataagg atgatttcaa tttctggatt gacttcgcgg gcgcaggcga
    37381 tgcacttctc aacgtcaaag ttgagatacg gcaacaggtc aactttgttg agcagcatca
    37441 gcgaggcggc ggcaaacata tgccgatatt tcagcggttt gtcttcacct tcggtaacgg
    37501 aaagcaccgc tactttgtgt ttttcaccga gatcgaagct ggccgggcat acgaggttgc
    37561 caacgttttc gataaacaga ataccgttat cgtccagtgg cagacgcggt gcggcgtcgg
    37621 caatcatctg tgcgtcaaga tggcagcctt taccggtgtt cacctgaatc gctggtgtac
    37681 cggtagcgcg aatgcgtgcg gcatcgttca cggtttgctg gtcgccttca ataaccgcgc
    37741 acggaacgct gtctttcagg cgcattaagg tttccgtcag cagggtggtt ttaccggaac
    37801 cagggctgga aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc
    37861 gttcagccag acggttgttt ttgtccagca cgtcaatttc gacttccagc atccgacgct
    37921 ggctcatgcc cggtgcgtga gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt
    37981 cagtctggct gggggtaaat tcaggcgctt taatgccggt gattttcatc tgcgggcgtg
    38041 ccgccggggc aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcga
    38101 tatacaggtt gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt
    38161 ctatccgccg aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacact
    38221 gtggacagcg gcggacgcgc tgggtcagta gcgtcacata ctgttggcat gtttcacacc
    38281 agcattcggc ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc
    38341 aaaccagatc aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt
    38401 tgagccagac cccagttacg cattttgcgc cgtgttttgc ggcctgctgt tcgatcaatt
    38461 ccagtgcccg ttggcagagg gttatttcgt gcatatcgcc tcccattaac tattgccagc
    38521 tacaagcaat aattgcgcca gtgttgatta tccctgcggt gaataatgtc gatgatgtcg
    38581 aaatgacacg tcgacacggc gacgaaattc atctttagct taaaaatctc tttaataaca
    38641 ataaattaaa ggttggcaca aaaaatgctt aaagctggca tctctgttaa acgagtaacc
    38701 tgacaatgac tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc
    38761 gtctacagga ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga
    38821 aagcgcgcct gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt
    38881 ttgaacattt tcgtatttac gtcaccctgg cggatggctt taacagccac accatcgagt
    38941 attacgtcga aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca
    39001 ttgacggcat gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact
    39061 atctcgaaaa aatcgctggc gtttacgaca gcttatacac cgccattgaa aacaatgtgc
    39121 cggtgaattt aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc
    39181 ccgttttgca ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac
    39241 atttcatcgg catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt
    39301 tttgtaattg ccgactccac gctctgtatc ggctgccaca cctgtgaggc cgcctgttca
    39361 gagacgcatc gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctcaatgaa
    39421 aaagaatctg cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc
    39481 ccggttaacg ccatcacccg cgttgatggg gccgtgcagt tgaatgaaag tctgtgcgta
    39541 agctgcaaac tgtgcggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt
    39601 ccgctggata ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg
    39661 gcgcgtgtca gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt
    39721 gacctttgta gctttgatga acaaggtccg gcctgtgtgc ggatgtgccc gactaaagcc
    39781 ctgcatctgg tggataacac cgatatcgcc cgcgtcagca aacgtaagcg tgagctgacc
    39841 tttaacacgg actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa
    39901 tgagcgcaat ttccctgatc aatagcggcg tggcatggtt tgtcgccgcc gctgttctgg
    39961 catttctctt ttcttttcaa aaagcgctaa gtggctggat agccggaatt ggcggcgcgg
    40021 ttggtagtct gtatacggca gccgcgggct tcactgtact gactggcgcg gttggcgtga
    40081 gcggtgcgct gtcgctggta agctacgatg tgcaaatctc cccgcttaac gcgatttggc
    40141 tgattaccct cggcctttgc ggtctgtttg tcagcctcta taacattgac tggcatcgcc
    40201 acgcgcaggt gaagtgcaac ggcttgcaga tcaatatgtt gatggctgcc gccgtctgcg
    40261 ccgtcatcgc cagcaacctc ggcatgttcg tggtaatggc cgaagtcatg gccttgtgtg
    40321 cggtgttcct caccagcaac agcaaagagg gcaaactgtg gtttgcgctg gggcgtcttg
    40381 gcactctgct gctggtgatt gcttgctggc tgctgtggca gcgttatggc acgctggatc
    40441 tgcgcctgct ggatatgcgt atgcaacagc tgccgctcgg ttccgatatc tggctgctcg
    40501 gtgtgattgg ctttggcctg ctggctggga ttattccgct gcacggctgg gtgccgcagg
    40561 cacatgctaa cgcctctgcg ccagctgccg cgttgttttt tacggtagtc atgaaaattg
    40621 gcctgctggg catgttaacc ctgtcactgc tgggcggtaa tgcaccgctg tggtgggggc
    40681 gcgctgctgg tgctcggcat gatcaccgcg ttcgtcggtg gtctgtatgc gttgatggaa
    40741 cacaacatcc agcgcctgct ggcttaccac accctggaaa atatcggcat tatcctgctg
    40801 gggctgggcg ctggcgtaac gggtatcgcg ctcgaacaac cggcgctgat tgctcttggc
    40861 ctggtcggtg gtctgtacca tctgcttaac catagcctgt tcaaaagcgt actgttcctc
    40921 ggggcgggga gcgtctggtt ccgtaccggt catcgcgata tcgaaaaact cggtggtatt
    40981 ggcaagaaaa tgccggttat ctccatcgcc atgttagtcg ggttgatggc aatggctgcg
    41041 ctgccgccac tgaacgggtt tgccggggaa tgggttatct atcaatcctt cttcaaactg
    41101 agcaatagcg gcgcgtttgt tgcccgtctg ctggggccgc tgctcgctgt gggtctggca
    41161 attaccggtg cgctggcggt gatgtgtatg gcgaaagtct atggcgtcac gttcctcggc
    41221 gcgccgcgta ccaaagaagc cgaaaacgcc acctgtgcgc cgctcctgat gagcgtaagc
    41281 gtagtggcac tggcgatttg ctgcgtaatt ggcggtgttg ctgcgccgtg gctgctgccg
    41341 atgctctctg ctgctgtacc tctgcctctg gagcctgcta acaccaccgt ttctcaacca
    41401 atgatcacat tgctgctgat tgcctgcccg ctgctgccat tcatcattat ggcgattttc
    41461 aaaggcgacc gcctgccgtc acgttcccgc ggtgcggcct gggtgtgcgg ctacgaccat
    41521 gaaaaatcaa tggtgattac cgctcacggt tttgccatgc cggttaaaca ggcgtttgcg
    41581 ccagtgctga aactgcgcaa atggctgaat ccggtgtcgc tggtgccggg ctggcagtgc
    41641 gaaggtagtg cgttgctgtt ccgccggatg gcgctggttg aactggcggt actggtggtg
    41701 attattgttt cacgaggagc ctgagaatga gtgttttata tccgttaatt caggcgctgg
    41761 tgttatttgc cgttgcgccg ctgctctccg gtattacccg cgtggcgcgc gcccgtctgc
    41821 ataaccgtcg cgggccgggc gtgttgcagg agtatcgcga cattatcaaa ctgctgggcc
    41881 gccagagcgt tggcccggat gcctctggct gggtgttccg cctgacgccg tatgtgatgg
    41941 tgggcgtcat gctgactatc gctactgcgc tgccggtggt gaccgtcggt tctccgctgc
    42001 cgcaactggg cgatttgatc accttactgt atctcttcgc catcgcgcgt ttcttctttg
    42061 ccatttccgg tctggatacc ggtagcccgt ttaccgctat cggtgcgagc cgtgaagcga
    42121 tgcttggcgt gctggtcgaa ccgatgttgc tgcttggtct gtgggttgcc gcacaggttg
    42181 ccggttccac caacatcagc aacattaccg acaccgttta tcactggccg ctgagccaaa
    42241 gcatcccgct ggtgctggcg ctctgcgcct gtgcgttcgc cacttttatc gaaatgggca
    42301 aactgccgtt cgacctggcg gaagccgagc aggagttgca ggaaggtccg ctctccgaat
    42361 acagcggtag cggctttggc gtcatgaaat ggggtatcag cctgaaacag ctggtggtgt
    42421 tgcagatgtt cgtcggggtg tttattccgt ggggacaaat ggaaaccttc accgtcggtg
    42481 gactgctgct ggcgctggtg attgccatcg tcaaactggt ggtcggcgtc ctggttatcg
    42541 cgctgttcga aaacagcatg gcccgtctgc gtcttgatat tactccgcgc attacctggg
    42601 ctgggtttgg ctttgcattt ttagcgttcg tctccttgct ggcggcgtga ttaaagagag
    42661 tttgagcatg tctgaagaaa aattaggtca acattatctc gccgcgctga atgaggcatt
    42721 tccaggcgtc gtgctggacc acgcctggca gaccaaagat cagctgactg tcaccgtaaa
    42781 ggtgaactat ctgccggaag tggtggagtt tctctactac aagcagggtg gctggctgtc
    42841 ggtgctgttt agtaacgacg aacgcaaact gaatggtcat tacgccgttt attacgtgct
    42901 gtcgatggag aagggcacca agtgttgggt aacggttcgc gtcgaagttg acgccaacaa
    42961 accggagtat ccgtccgtga cgccgcgcgt tccggcggcg gtgtggggcg agcgcgaagt
    43021 gcgcgatatg tacggtttga ttccggttgg tctgccggat gaacgtcgtc tggtgctgcc
    43081 ggatgactgg ccggatgaac tttatccgct gcgtaaagac agcatggatt atcgtcagcg
    43141 tccggcaccg actaccgatg ctgaaaccta cgagttcatc aacgaactgg gcgacaagaa
    43201 aaacaacgtc gtgccgattg gtccgctgca cgtcacttct gacgaaccgg gtcacttccg
    43261 tctgttcgtc gatggcgaaa acattatcga cgccgactac cgtctgttct acgtccatcg
    43321 cggtatggaa aaactggcgg aaacccgcat gggttataac gaagtgacct tcctctctga
    43381 ccgtgtgtgc gggatctgcg gctttgccca cagcaccgcc tacaccacgt cggtggaaaa
    43441 cgcgatgggt attcaggtgc cagaacgcgc gcagatgatc cgcgccattc tgctggaggt
    43501 ggaacgcctg cactcgcatc tgctcaacct cggcctcgcc tgtcacttta ccggcttcga
    43561 ctccggcttt atgcagttct tccgcgtgcg tgaaacctcc atgaaaatgg cagagatcct
    43621 taccggtgcg cgtaaaacct acggcctgaa cctgatcggc gggattcgtc gcgatctgct
    43681 gaaagatggc atgatccaga cccgccagct ggcgcaacaa atgcgtcgtg aagtgcagga
    43741 gctggtggat gtgctgctga gtacgccgaa catggaacaa cgcaccgtcg gcattggtcg
    43801 tctggacccg gaaatcgccc gcgatttcag taacgttggc ccgatggttc gcgccagcgg
    43861 tctcgcccgc gatactcgcg ccgatcaccc gtttgtcggt tatggcctgc tgccaatgga
    43921 agtccacagc gagcagggct gcgacgttat ttcccgtctg aaagtgcgaa tcaacgaggt
    43981 ctataccgcg ctgaatatga tcgactacgg tctggataac ctgccgggcg gtccgctgat
    44041 ggtggaaggc tttacctaca ttccgcaccg tttcgcgctg ggctttgccg aagcgccgcg
    44101 cggcgacgat atccactgga gcatgaccgg cgacaaccag aagctgtacc gctggcgctg
    44161 ccgtgccgcg acctacgcga actggccgac cctgcgctac atgctgcgtg gcaataccgt
    44221 ttccgatgcg ccgctgatta tcggtagcct ggacccttgc tactcctgta ccgaccgcat
    44281 gaccgtagtc gatgtgcgta agaagaagag caaagtggtg ccgtacaaag aactcgagcg
    44341 ttacagcatt gagcgtaaaa actcgccgct gaaataagga atcgccatgt ttacctttat
    44401 caaaaaagtc atcaaaaccg gcacggcgac ctcgtcttat ccgctggagc cgattgcggt
    44461 tgataaaaac ttccgtggta agccagagca gaacccgcag cagtgcatcg gctgcgcggc
    44521 ttgcgtcaat gcctgcccgt caaacgcctt aacggttgaa actgacctcg ccacaggaga
    44581 gctggcgtgg gagtttaatc tcgggcgctg catcttctgt ggacgctgcg aagaagtttg
    44641 cccgacggcg gcgatcaaac tgtcgcaaga gtacgaactg gcggtgtgga agaaagaaga
    44701 cttcctgcaa cagtcccgct tcgcgctgtg caactgccgc gtctgcaatc gtcctttcgc
    44761 cgtacagaaa gagatcgact acgccattgc gctgcttaag cacaatggtg acagccgcgc
    44821 ggaaaaccac cgcgaaagct ttgagacttg cccggaatgt aagcgccaga aatgcctggt
    44881 gccgtccgac cgtattgaac tgactcgcca tatgaaagag gccatctgat gagcaattta
    44941 ttaggccccc gtgacgccaa cggcattccg gtcccgatga cggtggatga atccatcgcc
    45001 agcatgaagg cgtcgttact gaaaaaaatc aaacgttctg cctatgttta ccgcgtggac
    45061 tgcggcggct gcaatggctg cgaaatcgaa attttcgcca cgctttctcc gctgttcgat
    45121 gcagaacgct tcggcattaa agtcgttcct tcaccgcgtc atgcggatat tttactgttt
    45181 accggcgcgg tcacccgtgc aatgcgatcc cctgcgctgc gtgcgtggca gtccgcgccg
    45241 gacccgaaaa tctgtatctc atacggtgcc tgcggtaaca gcggcgggat cttccacgat
    45301 ctctactgcg tgtggggcgg tacggataaa attgtccctg tggatgttta tatccctggc
    45361 tgcccgccaa cgcctgccgc cacgctgtac ggctttgcaa tggcgctcgg cctgctggag
    45421 cagaaaattc acgcccgtgg gccgggtgaa ctggatgaac aaccggcgga gatcctgcat
    45481 ggcgatatgg tgcagccgct gcgcgtgaaa gtggatcgcg aagcacgtcg cttggcgggt
    45541 tatcgttacg gtcgtcagat tgccgatgat taccttacac agttagggca gggcgaagaa
    45601 caggttgcac gctggctgga agcggaaaac gatccgcgtc tgaacgagat tgtcagccat
    45661 ctgaatcatg ttgttgaaga ggcgcgtatc cgatgagtga aaaggtggtg ttcagtcaac
    45721 tgagccgtaa atttattgat gagaacgatg ccacgcccgc cgaggcgcag caggtggtct
    45781 attacagcct ggcgattggt caccaccttg gggttatcga ttgcctggaa gcggcgctca
    45841 cctgcccatg ggatgaatat ctggcatgga ttgccactct ggaggcgggc agcgaagccc
    45901 gccacaaaat ggaaggcgta ccgaaatatg gcgagatcgt catcgacatt aaccatgtgc
    45961 cgatgctggc caacgcattc gataaagccc gggcagcgca aacttcgcag cagcaggaat
    46021 ggagtacaat gctgttaagt atgctgcatg atattcatca ggaaaacgcc atctatttga
    46081 tggtgaggag actgcgtgac tgacgtttta ctctgtgttg gcaatagcat gatgggcgat
    46141 gatggcgctg gcccgctgct ggcggaaaag tgcgccgccg cgccgaaagg taactgggtg
    46201 gtgattgacg gcggtagtgc accggaaaac gacatcgtcg ccatccgtga actgcgcccg
    46261 acacgactgc tgattgtcga cgccacggat atggggctaa accccggcga aatccgcatc
    46321 atcgacccgg atgatatcgc cgagatgttt atgatgacta cccataacat gccgttgaat
    46381 taccttatcg accagttaaa agaagacatt ggtgaagtga ttttcctcgg cattcagccg
    46441 gatatcgtcg gcttttacta cccgatgacc cagccgatta aagatgcggt agaaaccgtt
    46501 tatcaacgac tggaaggctg ggaaggaaat ggtggatttg cgcagttagc ggtggaagaa
    46561 gaataattct ccatcaggga attcagggaa ttgaacatca gaacagggat gtgcgctttt
    46621 ttattgagct tagtgctgcc tgcacaggcc accagcttta ccgaatatct gccaatgagt
    46681 gacagcgaat acgcgcaaaa gcgggcgctg aaacctctcc tgaccatgcc ttatgatgcc
    46741 gagcaaacct ggcattttag gaaagtgggc gttgctggcg tgacgcttga gaagatgcca
    46801 aatgaagaag attactggca attaaccgct aaagaccgcg ccggtaaatc ctggactgtg
    46861 cctgtgggta tgctggcaaa catagcgggc aaagggcaac tctatcgcgc cgatcttgat
    46921 cgtaacggca ttcaggatct ggttatctgg ttatctggtt accaagtacc ggtaatggtc
    46981 tggctcctta tgcacattta attcttatga cgttcacgcg cgaaggccgt ccctgtgtct
    47041 ttgaacccag gggactctac accgcaagca aaactggcgt agatgatttg ctggatttgc
    47101 aaggcaatgg tcacacgcaa ttgctggata tgcagtttaa ttctggttac tggataacca
    47161 gtctgtatca ggtaaaagac gccaaatggc agcgtgttca cggctggttt gacaagttaa
    47221 gctatcccgc gctcacgcgt tttacttata cccccaaccg taaactcgtc ctcaaaccca
    47281 tcgccgggcg cgacccgcaa acggaagatc tggcgcagac gcagcgttgc ttgataaagg
    47341 gggatgtgct tgaggggtga accagaactg actttttgca gagtggctat gaggattttg
    47401 ggggtaatta tatcgcgcac aagtgtttac atattgtgaa cttggatttg atgagctata
    47461 gtttccgaac acataaggat agaactcgca gatgtggctg tttacttact ataaggaagt
    47521 taaaacttaa aaatgcacat catttctaaa gcgccatttg aagagtctgc gcgtaaatat
    47581 cctaacgacg ctctggctct tcaggcattg tatcgggtta ttaaggaaac ggatttttct
    47641 acgccagaag agatgcgaac ggcgtttccc aacctcgaca attttaaata tcgcaacaaa
    47701 tggtacgttc tggatgtagg tgggaacaac ctaagggtga ttgcctacat aaactttgtt
    47761 aataagcggt tctttgtgaa gcacataact aatcacgccg agtatgacaa actgacacgt
    47821 tactacagag agaacaaaga atgactgcta atgctgcacg cgctgttaaa gcaacaagag
    47881 aactggtcaa tgcagtcccc tttctgggag gtagtgactc agaagatgat tatcgtgaag
    47941 cactggaact ggtcgaatat ttgattgaag aagatgacac caatccactt atcgactttc
    48001 tggcgagccg cattgcagaa tatgaaaaca acaaccaaga gtttgcagag ttcgacaaag
    48061 ccgttgcggc aatgcctgtt ggcgtggcgt tacttcgcac gctgattgat caacataatc
    48121 tgacctatgc ggatttgaag aacgaaatcg gctctaaatc cctggtcagt cagattttgt
    48181 ctggacaacg atccctgacg atatcccaca ttaaggcact ttcagcccga tttggcgtta
    48241 agccggaatg gtttctttga tttttgacta atgccaaatg ctaaattgcc cgatgcgctg
    48301 cgcttatcgg gcctacatgt cgcacgaact atgtaggccg gataaggcgt tcacgccgca
    48361 tccggcactg ttacttactc taaatcttcc ccattactgg caatcacttt tttataccac
    48421 cagaacgatt tcttacgtgt gcgcgtcagc gtgccgttgc ctgcgtcgtc acggtctacg
    48481 tagacaaaac cgtagcgttt gctcatttca cccgtagagg cggaaactaa atcaatacag
    48541 ccccatgtgg tgtagcccat cagcggaatg ccatctgcaa tcgcttcgcc cattgcgcgg
    48601 atatgttcgc gtaaatagct gatgcgatag tcatcgttaa tctcgccatt ggcagcaagt
    48661 tcatcttttg cgccgaggcc gttttccacc agaaacagcg gcttctgata acgatcgtac
    48721 atcatattca tggtgatacg cagaccgagt ggatcaattc cccagcccca gtcgctcact
    48781 tgcagatacg ggttacgcag cgatttcacc acgttcgccg cactgctgtt gttggcgttc
    48841 atctccgccg aggcgcagcg cgaggcgtaa tagctgaaag agacaaaatc gacggtgttt
    48901 ttcaggatct catcatcgcc cagcgcttta tctatggtta cccctttttc gcgaaatacg
    48961 cgggcagagt aagccggata cgcgccgcgc gcctgcacat cgataaaaaa caggttttcc
    49021 cgatctttct ccagcgccgc ccagacatct tccggtttgc agctgtaagg gtagaagtta
    49081 ccgcccgcca gcatacagcc cacctgattt tgcgggttaa cctcatgggc gattttggtg
    49141 gctagcgcac tggcaaccag ctggtgatgc gcggcctgat atttcacctg atcctgattt
    49201 tcaccttctt caaacaccag acccgcgccg gagaacgggc tatgcaacat aatgttgatt
    49261 tcattgaagg ttagccagta tttcaccaga ccatcaaatg cttcaaagca ggttctggcg
    49321 tagcggctga aaaactccac cagcttgcgg ttacgccagg agccatattc ggtgaccaga
    49381 tgcatcggca catcgaagtg gcacaacgtg accagcggtt cgataccgta ctttttacac
    49441 tcttcaaaga caggacgata aaaagcaatg ccctgctgat tgggcgtgat ttcatcgccc
    49501 tgcggaaaga gacggctcca ggcaattgag gtacggaaaa ccttgaatcc catctctgcc
    49561 atcagggcga tatcttcttt ataacgatga taaaaatccg tcgcctcatg gctgggataa
    49621 aactcgtcat cgcgcaactg aaaacgtttt tccagcccca gtttcaccgc cattcgatgc
    49681 tcgccgtgtg ggatcatatc gacggtggtc agcccttttc cgccttcacg gaacgcacct
    49741 tcagactggt tggcggcaag cgcgccgccc cataaaaaac cttctggaaa tactgacatt
    49801 ttcatcctca attaagactt acttctttgg cgacggtcgg ttgtacgctc tgatgctttc
    49861 gcgcctgggc agccgcttcc tcaacaggaa tatcctcaaa gccgagcaac agtgtgagga
    49921 taaacgacag catcaccgcc agcgccatga ccgcgaacac ccagacgatg ctcattggat
    49981 tcgccggatc gaagaattgc acgctggtaa atagccccg
//
LOCUS       DADLSB010000011        48347 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.011, whole genome shotgun sequence.
ACCESSION   DADLSB010000011 DADLSB010000000
VERSION     DADLSB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.011"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN331_000655"
     CDS             complement(<1..209)
                     /locus_tag="JN331_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7258879.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN331_000656"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN331_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY7258880.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN331_000657"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN331_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY7258881.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN331_000658"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN331_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY7258882.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN331_000659"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN331_000659"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY7258883.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN331_000660"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN331_000660"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY7258884.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN331_000661"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN331_000661"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY7258885.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JN331_000662"
     CDS             complement(4598..5320)
                     /locus_tag="JN331_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7258886.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN331_000663"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN331_000663"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7258887.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="JN331_000664"
     CDS             6520..6738
                     /locus_tag="JN331_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY7258888.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN331_000665"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN331_000665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7258889.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="JN331_000666"
     CDS             complement(7472..7672)
                     /locus_tag="JN331_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY7258890.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JN331_000667"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JN331_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY7258891.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKTQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JN331_000668"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JN331_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY7258892.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="JN331_000669"
     CDS             10169..12082
                     /locus_tag="JN331_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7258893.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="JN331_000670"
     CDS             12082..13104
                     /locus_tag="JN331_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7258894.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="JN331_000671"
     CDS             13098..13316
                     /locus_tag="JN331_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY7258895.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="JN331_000672"
     CDS             13370..14239
                     /locus_tag="JN331_000672"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY7258896.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="JN331_000673"
     CDS             complement(14294..14698)
                     /locus_tag="JN331_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY7258897.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="JN331_000674"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="JN331_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY7258898.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="JN331_000675"
     CDS             15683..17773
                     /locus_tag="JN331_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258899.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="JN331_000676"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="JN331_000676"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY7258900.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGWARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="JN331_000677"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="JN331_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY7258901.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19741..20343)
                     /locus_tag="JN331_000678"
     CDS             complement(19741..20343)
                     /locus_tag="JN331_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY7258902.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="JN331_000679"
     CDS             complement(20333..20500)
                     /locus_tag="JN331_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY7258903.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="JN331_000680"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="JN331_000680"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY7258904.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="JN331_000681"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="JN331_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY7258905.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNNAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /locus_tag="JN331_000682"
     CDS             22891..25434
                     /locus_tag="JN331_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY7258906.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="JN331_000683"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="JN331_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY7258907.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="JN331_000684"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="JN331_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY7258908.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cobA"
                     /locus_tag="JN331_000685"
     CDS             26708..28081
                     /gene="cobA"
                     /locus_tag="JN331_000685"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7258909.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLQEAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28328..28495
                     /locus_tag="JN331_000686"
     CDS             28328..28495
                     /locus_tag="JN331_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY7258910.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28790..30022
                     /locus_tag="JN331_000687"
                     /pseudo
     CDS             28790..30022
                     /locus_tag="JN331_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            30043..31065
                     /gene="frlB"
                     /locus_tag="JN331_000688"
     CDS             30043..31065
                     /gene="frlB"
                     /locus_tag="JN331_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY7258911.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31115..31945
                     /gene="frlC"
                     /locus_tag="JN331_000689"
     CDS             31115..31945
                     /gene="frlC"
                     /locus_tag="JN331_000689"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY7258912.1"
                     /translation="MKTGMFTCGQQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31942..32727
                     /gene="frlD"
                     /locus_tag="JN331_000690"
     CDS             31942..32727
                     /gene="frlD"
                     /locus_tag="JN331_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY7258913.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32827..33558
                     /gene="phoB"
                     /locus_tag="JN331_000691"
     CDS             32827..33558
                     /gene="phoB"
                     /locus_tag="JN331_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY7258914.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33718..34803)
                     /locus_tag="JN331_000692"
     CDS             complement(33718..34803)
                     /locus_tag="JN331_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7258915.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34815..36119)
                     /locus_tag="JN331_000693"
     CDS             complement(34815..36119)
                     /locus_tag="JN331_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258916.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36131..36484)
                     /locus_tag="JN331_000694"
     CDS             complement(36131..36484)
                     /locus_tag="JN331_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY7258917.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36495..37373)
                     /locus_tag="JN331_000695"
     CDS             complement(36495..37373)
                     /locus_tag="JN331_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY7258918.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37370..38596)
                     /locus_tag="JN331_000696"
     CDS             complement(37370..38596)
                     /locus_tag="JN331_000696"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7258919.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38597..39760)
                     /locus_tag="JN331_000697"
     CDS             complement(38597..39760)
                     /locus_tag="JN331_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY7258920.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRGQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39844..40206)
                     /locus_tag="JN331_000698"
     CDS             complement(39844..40206)
                     /locus_tag="JN331_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY7258921.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40223..41128)
                     /locus_tag="JN331_000699"
     CDS             complement(40223..41128)
                     /locus_tag="JN331_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258922.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41398..42402)
                     /gene="trpS"
                     /locus_tag="JN331_000700"
     CDS             complement(41398..42402)
                     /gene="trpS"
                     /locus_tag="JN331_000700"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY7258923.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42395..43153)
                     /gene="gph"
                     /locus_tag="JN331_000701"
     CDS             complement(42395..43153)
                     /gene="gph"
                     /locus_tag="JN331_000701"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY7258924.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43146..43823)
                     /gene="rpe"
                     /locus_tag="JN331_000702"
     CDS             complement(43146..43823)
                     /gene="rpe"
                     /locus_tag="JN331_000702"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY7258925.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43841..44677)
                     /gene="dam"
                     /locus_tag="JN331_000703"
     CDS             complement(43841..44677)
                     /gene="dam"
                     /locus_tag="JN331_000703"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7258926.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44784..46070)
                     /gene="damX"
                     /locus_tag="JN331_000704"
     CDS             complement(44784..46070)
                     /gene="damX"
                     /locus_tag="JN331_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY7258927.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTELAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQVDLK"
     gene            complement(46162..47250)
                     /gene="aroB"
                     /locus_tag="JN331_000705"
     CDS             complement(46162..47250)
                     /gene="aroB"
                     /locus_tag="JN331_000705"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY7258928.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPRKMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47307..47828)
                     /gene="aroK"
                     /locus_tag="JN331_000706"
     CDS             complement(47307..47828)
                     /gene="aroK"
                     /locus_tag="JN331_000706"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY7258929.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48229..>48347)
                     /locus_tag="JN331_000707"
     CDS             complement(48229..>48347)
                     /locus_tag="JN331_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7258930.1"
                     /translation="VPLLGDIPWFGQLFRHDGKEDERRELVVFITPRLVSSE"
BASE COUNT        11644 a        13150 c        12088 g        11465 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag tgccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccaaga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctag tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcaatgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaagccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggctcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctgggtttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccaac cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctttg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggccca ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat agcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgat cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctggaa cgtatggagc tgattgcccc
    11041 cgcacacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaattgaat ctggtccccg gctcgcgcat tggtctgtta ggccgcaacg gcgcaggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttcctggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccttttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aatagtgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccctgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcggtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccttg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtacctgg
    14161 cggcaaaatg gggctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tttgctatta tgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aagtcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacactt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggtaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcaa aattgcttcc
    16021 tgcatcgctg ctcgccgcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accattgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg aaaaagcgct
    16261 gccaccgctg ctggtgcgcc agcaaaaagc ggtcgattta attactcagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaacaatac cgattacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttgcagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaaactggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcact
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgt gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtagcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg caacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccacgt cttatcgata ccattattgg
    17161 ttgtttgatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgatg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggacccg caaccaacgc cactggcctg gcagagaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcattcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggccatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatacaggc taaaccatcg gataaggcat tcacgccgca tccgacatat
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgctaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccaa cctctgcacc cgcatacagg aaatcacgcg
    18001 cccaaccttt gtagtgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacaccgt agtacctgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgca tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcactg aatggcaatc
    19141 ggaaatcaga gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttgccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac atcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc
    21781 attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcact gggcaaacgt
    22021 gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac aacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccag cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25561 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    26041 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26161 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtagtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27061 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27181 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27241 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27301 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27541 gcaggaagag attaaccaga tcctgctgca ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27721 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27961 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28081 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28141 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28201 cttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28261 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28321 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28381 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28441 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28501 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28561 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28621 ttgataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28681 acattacata acatcatatg ttgttatatg catcatgcat tgtcatgtta ccttttaaat
    28741 gactgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28801 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28861 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28921 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28981 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    29041 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29101 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29161 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29221 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29281 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29341 caccactgcg attggcgcaa cgggcagctt tatggcgctg attggttcct gtctgctggt
    29401 tctggtgctc tacaccctgc tggcgctggt gatttccggc ctgatgccct tcgacaaact
    29461 cgccaattct gaaacgccga tttccgacgc cctgacctag atccccgcac tcggcagcac
    29521 cgctgggatc tttgttgcca tcacggcgat gatcgtcatt cttggttcgc tttccagctg
    29581 cgtgatgtac cagccgcggc tggaatacgc gatggcgaaa gacaacctgt tctttaaatg
    29641 cttcggccat gtgcatccga aatacaacac gccggatgtc tccatcatcc tgcaaggggc
    29701 gctggggatc ttcttcatct tcgtttccga tctcaccagc ctgctgggtt atttcaccct
    29761 ggtgatgtgt ttcaaaaata ccctcacctt cggctccatc atctggtgtc gtaaacgcga
    29821 cgattacaaa ccgctgtggc gtactccggc tttcgggctg atgaccaccc tcgccattgc
    29881 gtcaagcctc attctggtcg cctcaacctt tgtctgggca ccgattcccg gccttatctg
    29941 cgccgtcatc gttattgcta ctggtctgcc tgcttacgcc ttctgggcga agcgtagccg
    30001 ccagctcaac gctttgtcgt aatgttatct gaagaaaaaa aaatgttgga tattgataaa
    30061 agcaccgtgg actttctggt caccgaaaat atggttcagg aagtggaaaa agttctcagc
    30121 catgacgttc cgctggtgca cgccatcgtg gaagagatgg tgaagcgcga cattgatcgt
    30181 atttatttcg ttgcctgcgg ctcgccactc aacgcggcgc aaacggcgaa acatctggcg
    30241 gatcgctttt ccgatcttca ggtctacgcc atttccggct gggagttctg cgataacacc
    30301 ccgtatcgcc tcgacgatcg ttgcgcggtg attggcgttt ctgactacgg taaaacggaa
    30361 gaggtaatca aagcgctgga gctgggccgg gcctgcggcg cactcactgc ggcgttcacc
    30421 aaacgcgcgg acagcccgat tacctcggcg gcggaattta gcattgatta tcaggccgac
    30481 tgtatctggg aaattcacct gctgctctgc tacagcgtgg tgctggagat gatcacccgc
    30541 ctcgcgccga acgcggaaat cggcaagatc aaaaacgatc tcaagcagtt gccgaatgcg
    30601 ctcggtcatc tggtacgcac ctgggaagaa aaaggccgcc agcttggtga actggccagc
    30661 cagtggccga tgatttatac cgttgctgcg ggtccgctgc gtccgctggg ttacaaagaa
    30721 ggcattgtaa cgctgatgga atttacctgg acgcacggct gcgtgattga gagcggagag
    30781 ttccgccatg gcccgctgga gattgtcgaa ccgggcgttc cgttcctgtt cctgctcggc
    30841 aatgatgaaa gtcgccacac caccgaacgc gccattaact ttgttaaaca gcgtactgac
    30901 aacgtgatcg tcatcgatta cgccgaaatt tcgcaagggc tgcacccgtg gctggcaccg
    30961 ttcctgatgt tcgtgccaat ggagtggctc tgctactacc tgtctattta caaagatcac
    31021 aacccggatg aacgccgcta ttacggtggt ctggtggaat attaatccct ctcccggccc
    31081 ggcaacgggc cggatttacg caaggagtta cccgatgaaa acaggtatgt ttacctgcgg
    31141 ccagcagcgg ctgcctattg aacacgcatt tcgtgatgca agcgagctgg gttacgacgg
    31201 catcgaaatt tggggcggtc gcccgcacgc gttcgcgccg gacttaaaag cgggcggcat
    31261 caaacaaatc aaggcgctgg cgcagacgta tcagatgccg attatcggtt acacgccaga
    31321 aaccaacggc tatccgtata acatgatgct gggcgatgaa catatgcgtc gtgaaagcct
    31381 ggacatgatc aagctggcga tagatatggc aaaagagatg aacgcgggtt atacgctgat
    31441 ttctgcggcc cacgcgggct atctcacgcc gcctaacgtt atctgggggc ggctggcaga
    31501 gaacttaagc gaactgtgtg agtacgcgga aaacatcggc atggatctga tcctcgaacc
    31561 gttaacgccg tatgaatcga acgtcgtgtg taatgctaat gatgtgcttc atgcgctggc
    31621 gctggtgcct tcgccgcgcc tgttcagcat ggtcgacatt tgcgcgccgt atgtccaggc
    31681 ggaaccggtg atgagttatt tcgacaaact gggcgataaa ttacgtcatc tgcatattgt
    31741 cgacagcgac ggggccagcg acacgcatta cattcctgga gaaggcaaaa tgccgctgcg
    31801 ggaactgatg cgcgatatta ttgatcgggg ctatgagggt tactgtacgg tggagctggt
    31861 gacgatgtat atgaacgagc ccagactcta tgcccgccag gcgctggaac gctttcgcgc
    31921 gctgctgccg gaggatgaga gatgaaaacc ctggcgacaa tcggcgataa ctgcgtcgat
    31981 atctacccgc aactgaataa agcgttttct ggcggtaatg cggtcaatgt ggcggtgtac
    32041 tgcactcgct acggcataca gccgggatgc attacctggg tgggtgacga tgactacggc
    32101 acaaagctga agcaggatct cgcccgcatg ggcgtcgata tcagccatgt ccatacgaaa
    32161 cacggcgtta ccgcacaaac tcaggtggaa ctgcacgaca atgatcgcgt ttttggcgac
    32221 tacaccgaag gcgtgatggc cgactttgcc ctgagtgaag aggattacgc ctggctggcg
    32281 cagtatgaca ttgtgcacgc ggcaatctgg ggacatgcgg aagacgcatt cccacagctg
    32341 cacgctgcgg gcaaacttac cgctttcgac ttctccgaca agtgggacag cccgctctgg
    32401 cagacactgg tgccgcatct cgattttgcc tttgcctccg caccgcaaga agacgaagcg
    32461 ctgcgtctga agatgaaagc gattgttgcc cgtggcgcag gaacagtgat tgtcacgctg
    32521 ggtgaaaacg gcagcattgc ctgggatggc gcgcagttct ggcgtcaggc tcctgaaccg
    32581 gtgacggtta tcgacaccat gggtgccgga gattcgttca ttgccggatt cctttgcggc
    32641 tggtctgcgg ggatgacatt accgcaggcg atggcgcagg gaacggcgtg cgcggcgaaa
    32701 accattcagt accacggtgc ctggtaggta taacgttggc gtgagcatct tcacgccaac
    32761 gtgctgttac ttgccggaaa acgaccctat aatccgagta attcattctt tatttcaggg
    32821 ccgattatgt cagctacgga ccgctactct catcaactcc tctacgctac cgtccgccag
    32881 cggctgctgg atgatatcgc gcagggggtt taccaggccg ggcaacagat ccctaccgaa
    32941 aacgagcttt gtacacaata taacgtcagc cgcattacca ttcgcaaagc catcagcgac
    33001 ttagtggcag acggcgtact gatccgctgg cagggaaaag gcacctttgt acaaagccag
    33061 aaagttgaaa acgccctgct tactgtcagt ggttttaccg attttggcgt ctcacaaggc
    33121 aaggcgacga aagagaaagt gatcgaacag gaacgggtca gcgccgcgcc gttttgcgaa
    33181 aagctgaaca tccccggaaa cagcgaagtg ttccatctct gccgggtgat gtatctcgat
    33241 aaagagccgc tgtttattga tagttcatgg atcccgctgt cgcgttatcc tgactttgat
    33301 gagatttacg tcgaaggaag ctccacctat cagttatttc aggagcgttt tgacacgcga
    33361 gtggtcagcg acaaaaagac catcgatatc tttgccgcca cccgcccgca ggcaaaatgg
    33421 ctgaaatgcg aactgggcga accgttgttt cgcatcagca aaatcgcctt tgatcagaat
    33481 gacaaaccgg tgcacgtctc cgaactcttc tgccgcgcca atcgcatcac cttaacgatt
    33541 gataataaaa gacattaacc gtaggccgga taagatgcgc cagcatcgca tccggcgatg
    33601 ctggcgcact gaattttaca tcccgcacgt tcccctcacc ctaaccctct ccccaaaggg
    33661 gcgaggggac cgttcagtgc ggaatttagc ttgattgccg aacggtgcta tccagcatta
    33721 aatactcgca atactctcac gcaaaatccg cagcaccgtc tcttcaccgc tgcgattcgg
    33781 gttaatgcga atcgcacaat gttcgagttg tggattcgcc tggcgaaacg ttcctgaaag
    33841 tcgatagaag agcggcggga tttcatattt cgactctgca ccgaccgggt aaggcaacgc
    33901 gccacgcttt tgcgcttctt ccagcaccct ggcggcaatc agctgatgaa actcgacaat
    33961 caacaccttc gactgcgcat tggcaatgac ggcgcttttc acttccgcca ccgcgccgcc
    34021 gttaagcaaa gccagcaacc gttcggatac tcccgcctgc accgcatgca tcactggcgc
    34081 gaacaccaga ccacgcaaga cttccagcgc ctgtgagccc tggatctggc taccgccgga
    34141 gtaaagcgtg gcgcgaatcc gactaataac atcggcatcg ccaaccactg caccaacgcc
    34201 ctccggccca aacagcttga agcaggagaa tgtcgagaca ttcgcgccgc attcacagcc
    34261 gattcgcgct accttcatca ccgcatagtt atcatcggtt aacgctggaa cacctgccgc
    34321 gcgcaacgtt gccagcacat ctgccagcac atagctgtcc tgcggctgct ggcgcgtatg
    34381 ctgcaccagc gcggcatcag gttgttgctc gtcgacgacc tgcttcagtg ccgacaggtc
    34441 attgaaatca gcagtaataa gcgtcagccc catctgctca ataataaccc gtgtcgtcgg
    34501 gtaaacaggc gcgtcatgca ccagaagacg ctgccccggt ttgagcaaag ccgccagccc
    34561 ggcgcgaatc gcgccagtcc ccgcgccctg caccagcgcc gcggcctgtg cgtgaaatgc
    34621 atcagccagc acctgctcca cccgttgggt aatacgcggt tgattcagac ctggagttaa
    34681 ccctaaatca ccgccagcaa gaaactcgct gcctggaaaa tggcggcaaa tagtgtccac
    34741 cagcgcaaac tgcttttgct gcgcttcagc aagagtcagg ctttgcaaag gaaacgtctt
    34801 cataggtgcc ttccttaagc cggaacgaac aggccaagcc agtaaagaac attcagcaga
    34861 ataccggtga tcattactgc cacgaccggt gccgccattt tctgtaccgg acggcccagg
    34921 gattcgttga ggaagtaaat ggcaaccgcg atagagaatc cggtataacc cgccatctta
    34981 attgctgcga aaatcgaacc gaccagcagc gccacttcca tcagcatatt catggcgtta
    35041 cggatgttat ccgacgcatt acgcaccgac gggtagcgtc ccagccattt gccgatcgaa
    35101 cgaagcagca agacttccgc cgaaataacc actgcgccta ataccgctgc aaccatcgga
    35161 ttcggcgaga gatagcccac cgcataaaca aaggtaaagc ccgcaactgc atacacaccc
    35221 gttgctaagg cggtggtggc aatcaacggc acaaacccca gtccgcgcat aaattctgcc
    35281 agagccgcct gattaatcag cgtttgcgat tgttccggcg tcacgcctgc ggaatacgct
    35341 ttctccagtg tgaagatcga aacttcgcta ccagcaaaaa tcttcatact ggcaacggcg
    35401 gcaatcaatg ctcccacgat ggcgatataa ggtaagtttt tgataatccg cgacgtgcgc
    35461 tcttcaaaca ccgaaagccc gctggcatcg atgtcatttt catcacgatg acgcaggtcg
    35521 tgagttatcg cgatccccag cagcatcacc atgccaataa agatttcgat ggattcaggg
    35581 ttaagatgtg ggaaatagcg cacgactacc acacgggtca tcagaacaac cacggcggcg
    35641 accagacttt gcttccagcc aaactggtag aaaatcgcca ccagcgggaa cagtgcaaaa
    35701 gcagaaacca ccggcgagct taattccccg aggctaccta atacatccac cggcagcgcg
    35761 gtcagcagct ggttaactgg caacaggcaa gtaaggatca acacgcccca gatagcgcca
    35821 agaccaaacg ccatcaggct gtttatcgcc agtacgccga ggatatcggt aggaagaaag
    35881 agtaaccatg cgttgagcag cccggttttc agggtaaaag agatccccac cgaggccaca
    35941 aaaccaatgc tcagaccaaa agcgatactc cccgcctcgc gacggttcat atagccttca
    36001 atcagttgcg gcaggatcgg gtggatgccg tcatgaaaaa cagccgccga gcgatgcgcc
    36061 agaagcgatg tcatacccgt caggcacgcc accacgataa tctgaatata cagatccata
    36121 gtcgtgcctt ttatttcaga tggttaatca acatcggaat cgcgtgttcg acgtgctcaa
    36181 cggaaaggcc aaacgccact ttcccttcag cgatcatttt ggcgatatgc tcgtctttcg
    36241 ctttaatgcc tggtttggca atggtgcaac ttttgttata gccaatcacc gcgatggcaa
    36301 tcgacaatgc cgcgcccgca ccggtattac acgcgccaat gtaataatct agttgcccgg
    36361 atttcacctt cattgccgct tccatgtcgt tgtgaatgaa aacttcaaaa cagccaggag
    36421 ccgtcgcttc aatggttttt ttaatctgct cacgctgtaa gcctgcaacg ccaatctttt
    36481 tcatgagtct gtccttattg gaaaaattga gagggatttt cacgtaacat cacatccaca
    36541 tcggcctgac tgaatcctga ctggcgcaat tgcggaataa aggtggtcaa taaatagtca
    36601 tagccataac caccattggc ttttaaatgg gagcggcgcg taatatccat cgacagcatg
    36661 acgcggttca gcaacccacg gtcacgtagc gcgtggagca tcgcaatgcg cttttcgtcc
    36721 gggtagtaac tgttcttacc aatggtgtcg aactgcacgt acgcgccgag atcgatcatc
    36781 ttcaaaatgt tgtcgaggtt gtctttcaga tcgcagtgac caacggtgac gcgcgaaaga
    36841 tcaactccgt gggcttgcaa cagagccagt tgttccagcc ccatggtgct gaacgacgta
    36901 tgcgtggaga tcgggcgtcc ggtctggtta tgcgccagcg cagcggcaat aaataccttc
    36961 tcttccagcg gcgtaatctt tccttcgctg gagccgatct ccgcgatgat cccggctttc
    37021 agctccgtgc catcgatacc ctgttcaatt tcatcgacca tctcctgcgc cagttcctgc
    37081 acgctgcggg tcaccacatg ttccgggaaa aacgcatcct ggtaataacc agtacaggcc
    37141 accacgttga tcccggtttc gcgcattaca tcgagcataa attgcgcatt gcgccccatg
    37201 taacggttgg tcatctcaat cacattacgt acaccccggg tcatcaggtc gttcatctcc
    37261 tggcaaatga acgcatactg atcaaggcgg cagtccacgt tgtttttaaa gccggagaga
    37321 tcaatatgca gatgctcatg ggccagggtg taacccgtcg gatcaaaact catagggtgt
    37381 ctcctgcaaa ccggagggag gaaagaaaag agcgaccatt ttgtggcggt ggcgcacgga
    37441 aaaattcaca cacggtagct cccacatcag aaagcgtggt gcgcacaccg agctgcgtgg
    37501 cgaccagccc ttgctgataa accagcactg gcaccacttc acgggtatgg tggctgtgac
    37561 caatggtcgg atcgttaccg tgatccgcca tcacgaccag gcaatcatct ggctgcatcg
    37621 cctcaacaag ccgggcaagg ttacggtcaa cgacctgcaa acgttcggca taacgtgcga
    37681 cgtcttctgc atgaccagcg aggtcggttt cctggatatt gatgcaaata aacgccgtcg
    37741 gatgggtgtt aaattcgtcg agggtgatat ccataatccg ctggctatcc accaggtttt
    37801 gccagctcac accataagga ttgctgacga tatctgccac cttacccacc agcacggttg
    37861 gcacgcccgc ttcatacagt ttttgcggca cctgcacttt ttcatcaaca ccgtagccca
    37921 tatgcacgac ctggaagccg ttgtcataag cgccagaacg cggcgcatta ataccaataa
    37981 agcgcccttc tttgctttct gcggcatcga gaatacgctg actgttggtt aacaggccgc
    38041 caaatgtaat gacccgaccg acctgtacct ggtcacgcac gatacgacca attttgattg
    38101 cgtcgtcaaa agagatcaca gagagattgg cggtaatgtt atagacttgg cctaaatccg
    38161 cctcgagatt atcgccaatc gcaaccgcct gattgaccca cagaaattgc agatcatcgc
    38221 cacggcgctc cacctgccag ccagcggaaa ctaatgcctg ctcaacacgg tcaatcacat
    38281 cgcgaaaagg catccgcagc ggcggtaacg ggcgcgtgcc taaaatttcc tgatgcccca
    38341 taaaggtatc gccaccttca tgttgcagct ctgccacgcc ccaggttgcg gaatctgacg
    38401 gctgcatatc gcctggcgca taacccaatg tgttgattag ccccagcttc tccagcgttg
    38461 gtagctgcaa atgcggcaac tggctcagga tgtgaccaca ggtattcgcc ccggcatctt
    38521 gcgggcgcac cagcgtgaca tctttcattg cccctacgcc aaagctatca atcactaaca
    38581 ccacaaatcg cgccatttat gcctccagag ggttgccgag gctgtcataa cgaccaacta
    38641 tttctggttc gccgcgctga ataccggaca ccagcaccac atcgctacgg gtgacaaaaa
    38701 tctgcgtgcg aaaacagagc accactgcac tgctcaccgg atactcgcct gccagcggca
    38761 gggtgtagtc gatactgctg tcatccacag ttttgagatt ggtttcagta atcttttgat
    38821 tttctggcgt aaacaccagc gcatgttgcg catgaccacg acggtagtaa ccgccgccgt
    38881 agcagtagct gtcgccacgg aaatgatggg agatttcact taaccagagc atcgcgatac
    38941 gttcaggctg atcgccctgc tggtttgccg gaatagtacc ggtcagcgca tgaccgggtt
    39001 cggcatgagt cgcaccgtat tccgccagca atggcagcga agtgcagctg gtcgctgaag
    39061 gtacgttcag ttgctcaatc gcaataccag atttcgccag ttgatcccgt gcttgtacca
    39121 gcgtgtgaag attcggtgtt ggcaaaactt tcccggcagc ctcatcccaa agcaggcaag
    39181 ggaaatgggt tagtccggct aaatgcagcc ccggcagttt cttaatttca gcaatcacct
    39241 cgtgcagcga atgctgtaca aaaccgctct cctgccccgg ataaagaaaa tcatcgtcgc
    39301 tataaacttt gagcagcaca ggctggcccc gtccggcctt aaccgctgcc gcagaaactt
    39361 cccgcgcttt gtcgagagta aacacggtga tgatgtcggt gccctgttca acggcgtcag
    39421 caacctgatg acaagggatt tgtaccagat gcccctgatg cgccactggc aaaccagcgc
    39481 ggcgcatgac tcgcgcctct ttgtaatcca ccgccacaat gccgctgtag cctaatgcca
    39541 gcaatttttc cgccagccac ggattacgac caaattgctt ggtcatcaga tacagttcaa
    39601 tgccgtaaag ccgcgccgtc tcaatgagcc gcttaccgtt ttccagtatc tgatccacgt
    39661 cgatcaccca gctgtccggg gcgattttgc cctgctgcca caaacttagt gcggcggaaa
    39721 tcagcgccgg gttctggcgt ttcaatgctt ctacaaacat cttgttgcct cgtttctgtt
    39781 ccttgaatta aatattcatt tttttgaata tttaggttgt gcgacgtgga tttgtcggga
    39841 gcttcaaacc tcttcgttag cagccatcca taatccataa agattggcca gcaaatagcc
    39901 ttcttcacac gggttaactt ccagagcgaa ttccttcaac aacacctgat gcaattgcac
    39961 aacggcttgc cagtggctgg attgcgccag ttccgccagc aactcgttat ccagcggctc
    40021 tatttcttca ccacggcgac tgcgcatcag tgcactcgcc atatgtgtca tcgccatcgt
    40081 tccttgctca ctgcgcaccg gcagatggca ctctttttcc agtacgttga cgacctgcat
    40141 catgcctttg cagacgtcct tatcaataac gcctgcctcg caaagcaggt tgagtctggt
    40201 ttccatatca gatgcctttc gtttaataac tgggttcctg ttccccactc accacccgtt
    40261 gttgatgggc aagcagggcg tgtttatcta caacggcacg cagcaattgt tgcattgggt
    40321 aatcatcgac tcgcgtcagg accacggctt cggtggcctg taaaaatcgc ggatcgtctg
    40381 ttagcggcgt cgcctctaat cccagcatgg tcagttcgtt ttccgccacc acgttccaga
    40441 tcaccgcatc gacatcgcct ttaacaatgc gttgtaaact ctcgtgataa gagagatcga
    40501 ctcgttccac atcactatcg ccaaaaaaga catcggtcat gattttctga tccgccgaac
    40561 gactatcgag acctactcgc ttaacatttc cagactcacc tttgcggcat atcagttgat
    40621 gttcaccaac atacgtatgc gggccaagct cgagggcgat gcacaaattg ttttggctga
    40681 gataactttc cgccgccagg cgggaaacta ctgccatgtc atagacaccg ttaagtaaac
    40741 actccacgcg aatatccgcg ccgcgcatat gcgcatagta aaaaggaatg ccatcaaact
    40801 gggctttcaa tccgctcgct aaaccttcgt acaagcgggt atagggcaaa ggcatcgcac
    40861 ataccacgtt gttgatatcc acatgactca gcaatgcttt gttatccatc tcgacaagat
    40921 aactgccatt gcgcccacgg cgttcaatcc gtatcgctcc gcttgattcc agcgttttca
    40981 acgcggcctg cgtcaggcca accgatgaac ggcattcagt tgccagctca tctatggttt
    41041 tcagtcgatt accgcatttt tcacccaaca aataacgggc cagtgtcgtg atgacgacgc
    41101 cttctttttt gataaacgtt cgacgcataa tatattttca ataaaatgaa tatttatatc
    41161 ttcattaaaa tgaagataag tggcaacgac gagatgtgga aacggcgagg cgcttcacat
    41221 tttttcgggt tgttgcgaaa agaggataaa aaaaccgggt ttccccggtt tcagagtgat
    41281 gacgaagtga tttattcgac aatgcgagca aagggtaatt cccactggcg cttgcctgct
    41341 cccctcaccc taaccctctc cccaaagggg cgaggggacc gtccgagcgt tttgatatta
    41401 cggcttcgcc acaaaaccaa tcgcttcgta caccgctttc agcgtacggg aagcgtgtac
    41461 actggctttt tctgcgccgt ctttcatcac ctgttgcagg aaggcttcat cgttgcggaa
    41521 acggtgatag cgttcctgca attcagtcag cataccggaa acggcatcag ccacttcacc
    41581 tttcagatga ccatacatct tgccttcgaa ctgtttttcc agttctggga tgctctggcc
    41641 cgttaccgca gaaaggatat ccagcaggtt ggaaacgccc gctttgttct gcacatcgta
    41701 gcgaactacc ggcggctcgt cggagtcagt gaccgcgcgt ttgattttct tcactaccga
    41761 tttcggatct tccagcaggc cgataacgtt attgcggtta tcgtcagact tggacatctt
    41821 cttggtcggc tccagcagcg acattacgcg cgcgccagat ttcggaataa acggctccgg
    41881 caccttaaag atctcgccat acagcgcgtt gaaacgctgg gcgatatcgc gactcagttc
    41941 caggtgctgt ttctggtctt cacccaccgg taccagatta gtttgataca gcaggatgtc
    42001 cgcagccatc agcaccggat agtcaaacag accagcgttg atgttctcgg cataacgcgc
    42061 agatttatct ttaaactgcg tcatgcgact cagttcgccg aagtaggtat agcagttcag
    42121 tgcccagcct aactgcgcat gttctggtac gtgggactga acaaaaatgg tgcttttctc
    42181 aggatcgata ccacaagcca gatacaaggc cagcgtatcc aacgtcgctt tacgcagctt
    42241 ctgtgcatcc tggcgcacgg tgatcgcgtg ttggtcaacg atacagtaaa tgcaatggta
    42301 gtcatcctgc atgtttaccc actgacgcag cgcacccatg tagttaccaa tggtcaattc
    42361 acctgagggc tgtgcgccac taaaaacgat gggcttagtc atttttcgat tcctgatttt
    42421 cgctatgcgg aagccctaat gcgggcagaa ggtcatttat agactggtaa attacatcag
    42481 gctggctgag atcgatagcc tcgccgtagt tatatccgta ggttaagcca actgatgggc
    42541 aacctgccgc ttttgccgcc tgaatatcat tgcgtgagtc gccgacaaac agcatctgtt
    42601 gtggggcaat tcccatccgc tcagccacca gtaacagcgg gtccggatgc ggttttttgt
    42661 tttgcacatc atcgccgccg ataaccacgc taaagtattt ggcaatatct aaggcttcga
    42721 gcagcggcgc gacgaacggc gtcggtttgt tggtgaccag gcctagcggc aggcctttag
    42781 cctgcaacgc gcccaacgta tcggcaacgt gcgggaacaa aaacgtcccc tcttcggcaa
    42841 cctcgccata gtagcgatcg aacagtttac gcagaatacg tacctgttct tctgccggaa
    42901 tgtcgtcatc aacgggcggt ttccccattg ttttacgcag agtcgcacgt tcctgacgcg
    42961 cccaggtcaa tgcgcgctcc atcagaacat ctgcgccgtt accaatccag gtaataacgc
    43021 gttcttcacc tgcgacgggc aactccagcg catacagcgc catatctacc gcagcagcaa
    43081 gaccaggagc actgtcgacc agcgtaccat caagatcaaa agcgacgccg cgaatatctt
    43141 caaacttatt catgacttac ctttgccagt tcactgcgca tttcatcaat gactttttta
    43201 tagtctggct ggtcgaagat tgccgaaccg gcgacgaaca tatccgcgcc cgccgcagcg
    43261 atttcgccaa tgttgttcac cttcacgcca ccgtccactt ccagacgaat gtcaaagcca
    43321 gactcgtcga tacggcgacg tacttcgcgc agtttatcca gtgtttgagg aatgaaagac
    43381 tgaccgccga aaccagggtt gacggacatc agcaggatca catccagctt atccatcacg
    43441 taatccagat agctcagagg tgtcgccggg ttaaatacca gacccgcttt acagccattt
    43501 tctttaatca gttgcagcgt gcggtcaaca tgctcggagg cttctggatg aaaggtaatg
    43561 atgctggcac cagcggcagc gaaatcaggc acaatgcgat cgacgggttt caccatcaga
    43621 tgtacgtcga taggggcggt aatgccgtag ttacgcaagg atttcagcac cattggccca
    43681 atcgtcagat tgggaacata gtggttatcc atgacgtcaa aatgcacgac atcagcgcca
    43741 gctgccaggg cttttgcggt atcttcaccc aggcgggcaa aatcagccga cagaattgag
    43801 ggggcaatca aatactgttt catccgcttc tccttgagaa ttattttttc gcgggtgaaa
    43861 cgactcctgg tttgtacaaa gccagcagtt cgtccacctt tttacgtgtg ccgccgttgc
    43921 tgcttatact gcgtcgaact ttgacgacat gcaattttgc gcgctgatac cactcacgcg
    43981 ttaacatcgt atcgtgattg gagatcagca ctggaatatg gcgctcaacc agaccttcgg
    44041 cgatctccgc cagatgcgct tgttgttcaa gcgtaaaact gtttgtgtga tacgccgtaa
    44101 agttggcggt cgcagacagc ggtgcataag gcggatcgca atagacgacg gatgcatcat
    44161 ctgcgcgcgc catgctatcg gcgtaagact cacaatagaa aaaggcattc tgcgcttttt
    44221 cagcgaagtg atacaactct gcttccggga aatagggttt tttgtagcgg ccaaacggca
    44281 cgttaaactc accgcgcaga ttgtaacgac acaggccgtt gtaaccgtag cggttcaaat
    44341 ataaaaacag taccgcccga cggaacggat cctggctttt gttgaactct tcgcggaact
    44401 gatagtaaac ctcggcgcaa tttgtttcgg gaacaaacag ctcgcgtgcg gcctgtacgt
    44461 actcatcagt acgcatcttc acaatgttat agagactgat caggtcgcta ttgatatcgg
    44521 caaggatata acgagaaaag tcggtgttga gaaacaccga cccggcacct acaaaaggct
    44581 caaccagaca ttcgcccttg ggcaaatgcc gtttaatatc atcaagcagg ggatacttgc
    44641 cccctgccca cttcaaaaaa gcgcgatttt tcttcatgct gactaactaa ttacaccttc
    44701 tccggctgtg gagaaagctc cgacagcatc cagcgcttca ggcagctccc gcaacgataa
    44761 accattgcgg gagataacct tgattacttc agatcgacct gtacctgacg cagcggtttc
    44821 gcccacgggt ttttggcctg aacatctgct ggcaatgtag ataccgcttt tttcgcctct
    44881 tctttcgaag cgtacacgcc agaaaccagg acataccacg gctgaccatt acgcgtcgtt
    44941 tcatagacaa cgtagttttt cagattctct ttcttcgccc aaccgttcag gttgtcgtag
    45001 ttagaggaac tgctcagctg cagagtgtaa tggctggacg gtgccgattt caacgaacca
    45061 acattacctg cggtcttccc tccagcggct ggtgttgccg tggtttgcgc cggggatgcc
    45121 gtctgtactg gagccgtagt cgccgtctct tttggtgctg gcgtagaagt cgcagccggt
    45181 gccttcgtgc tcgccacagg ggcagctagt tcagtacgct tcggcgtctg tgctaccggc
    45241 ttcggctccg ttttcacggt tgcttgcggt tttttcggtt caatcaccgc ctgctgacga
    45301 actgggcgcg tagtggacgg acgttcagcg gtttgcgttt tcgccgtgtc acgcgatgca
    45361 ttgccattgc gaacaggcgc aaccgttgcg ggttcagtcg gcaacgtgga attgaccgcc
    45421 acattgttca gctgttgctg attttgtggc tgggtcagcg cattgttcag gtcaccctgc
    45481 acttcaacac gttgttgacc atccgttgcc accggggttt gcccttgagt cggcgtagaa
    45541 gagatcggtg gcagagaaac atcctgctga gtattttccg cagacgtggt tcccggcgct
    45601 ggctgcacgc cattcgcctg atcggtcgca ttgccagcaa gatcaatact cttctcgcca
    45661 gacgcggttt gatcgctgga agtggtcgag ggggctttta gcgcagaacc gataccgatg
    45721 atcaacagca gtagaaccag aatgccgacg cccatcatca tatactgacg agaagcgggt
    45781 ttactggctg cttttttgcg cttacgcgga cgacgctcta cgcgctcttc atccacggtt
    45841 tcatcttcgg attcatcaat ttcttcttcg atttccggtt cctcattgcg ctctttttgc
    45901 gcacgagtcg gacggcgatc gtcagtgtca tcaagttcaa tatcatcaaa attgatctgc
    45961 ggttcgccac gttcagtacg ctcagaacgt tcagaagatt gacgagaacg accagtacga
    46021 cgatcgctgg gatcgggttt cagctcgtct tctggtttga attcatccat ttaacacccc
    46081 actaaaaggt taatgcttac cacgttgcaa ttaacctgaa gctaatagac cgcttgataa
    46141 gcggcctgac ctttcttgtt gttacgctga ttgacaatcg gcaatggcgt taagaacaag
    46201 ctcgtgcgaa acgccgctgc gaacttcact cttaccaatt gccaacggaa gaattaagcg
    46261 catctctccc gcaaggactt tcttgtcacg cagcatatgc ggtaaatacg cctgcgcgga
    46321 cattttgcgc ggcccattga ccggtaaccc agcccgcgtg agcagggtta taatacgctg
    46381 cgtttcggca gaactaaact gcccgagacg ttccgacgtc cgcgccgcca tcaccatacc
    46441 cgcagcgacc gcttcaccat gtaaccaatt gccatacccc atttcagctt caatggcatg
    46501 accaaaggtg tgtcccagat tcagtaaagc acgtaacccg gtttcgcgct cgtcggcggc
    46561 gacaacttct gccttcagtt cacaacaacg gcgaatacag tacgccattg ccggaccgtc
    46621 cagacgcaac aacgcatcca gattctcttc cagccagtta aaaaacgcac cgtcaagaat
    46681 aatgccgtat ttgatgactt ctgccagccc cgacgctaac tcacgcgggg gaagcgtttt
    46741 cagacagtcg agatccacca ccactgaagc aggctggtag aacgcgccaa tcatgttttt
    46801 accgagggga tggttgaccg cagttttgcc gccaacggag gaatcgacct gcgacagtaa
    46861 cgtcgtcggg acttgaatga aacgaacacc gcgctgataa ctcgccgccg cgaagccggt
    46921 cagatcgccc actacgccgc cgccaagcgc caccagcgta gtatcgcgac catgcggctt
    46981 ttgtaacaac gccgtaaaga cggtatcgag tacagccagg cttttatact gctcgccgtc
    47041 agggaggata acgctatcga cgttaacacc cgcctgttca agtacgccgc ggaccttatc
    47101 gagatacaga ggagccaggg tttcgttggt gaccaacatg acctgctcgc ccgatttcag
    47161 cggtaagaat gaagctggtt cattaaacaa accagatgcg atggtaattg ggtaactacg
    47221 ttccccgaga gtaacgacaa tcctctccat aacgcgacat ccaccttaat tactgtaccc
    47281 gcagacgagt gtatataaag ccagaattag ttgctttcca gcatgtgaat aatctggttt
    47341 gcaaccactt tagcgctttg atcatcagta cgaatggtca cgtcggcaat ctcttcatac
    47401 agcggattgc gttcattggc caacgcttcc agaacttcac gcggcggtgt ttcaacgtgc
    47461 agcaacgggc gttttttatc acgctgcgtg cgtgcaagtt gcttttcgat ggtcgtttca
    47521 agataaacga caacgccacg agcggaaaga cggttacgcg tttcacggga tttcacagag
    47581 ccgccgccag tagccagcac aataccctgt ttctcggtca actcattgat gaccttttct
    47641 tcgcgatcgc ggaagccttc ttcgccttct aaatcgaaaa cccagcccac atcagctccg
    47701 gttcgtttct caatctcttg atcggaatcg taaaattcca tattgagttg ttgagctaac
    47761 tggcgcccaa tagtgctttt tccggcaccc ataggcccaa ccagaaagat attgcgtttc
    47821 tctgccattt tttcggtact actaagacta ttcgttaatg ataaacccgc ttcgctcaga
    47881 gagcgccgca ggacatgaac tgaaacctca taagatattg cgagagtcag actgaaaatt
    47941 atctcaatac tccagcgggt ttggcaactg aataaatcac ccatccctct gcatatctgg
    48001 tcgctgcaag cgcgctgcct tgctaccacc gctctggcga taaatcaccg ggtaagatta
    48061 gcgtaaaaaa gacagcaaaa tgccgcctga atgataaatc atcatcatgg aacgcaggca
    48121 gcgaagagca ccaactctca aatcggtact ccttgtatgc taaatacctg cgcgcgtcaa
    48181 atagatgaaa cacgttcagc ataaaaacat taccgctttt acggctgttt actcactgga
    48241 aaccagtcgt ggcgtgataa acaccactaa ctcgcgtcgt tcatcttctt ttccgtcatg
    48301 acgaaataat tgcccgaacc agggaatgtc gccaagcaac ggtacgc
//
LOCUS       DADLSB010000012        47275 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.012, whole genome shotgun sequence.
ACCESSION   DADLSB010000012 DADLSB010000000
VERSION     DADLSB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.012"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..523
                     /gene="comR"
                     /locus_tag="JN331_000708"
     CDS             <1..523
                     /gene="comR"
                     /locus_tag="JN331_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY7258931.1"
                     /translation="EATSLADLVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAK
                     HEAQLFCEEKSVESALADYFAAIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKS
                     RHAMQERTLQQFLCQRQARGEIPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQI
                     AGTTLRLWPELVK"
     gene            complement(585..1124)
                     /locus_tag="JN331_000709"
     CDS             complement(585..1124)
                     /locus_tag="JN331_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7258932.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1351..2655)
                     /locus_tag="JN331_000710"
     CDS             complement(1351..2655)
                     /locus_tag="JN331_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAY7258933.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3063..3605)
                     /locus_tag="JN331_000711"
     CDS             complement(3063..3605)
                     /locus_tag="JN331_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7258934.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3628..4653)
                     /gene="nagZ"
                     /locus_tag="JN331_000712"
     CDS             complement(3628..4653)
                     /gene="nagZ"
                     /locus_tag="JN331_000712"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY7258935.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDAGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4664..5488)
                     /gene="thiK"
                     /locus_tag="JN331_000713"
     CDS             complement(4664..5488)
                     /gene="thiK"
                     /locus_tag="JN331_000713"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY7258936.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5469..6110)
                     /gene="lpoB"
                     /locus_tag="JN331_000714"
     CDS             complement(5469..6110)
                     /gene="lpoB"
                     /locus_tag="JN331_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY7258937.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQLGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSVQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6125..6502)
                     /locus_tag="JN331_000715"
     CDS             complement(6125..6502)
                     /locus_tag="JN331_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY7258938.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6505..6864)
                     /gene="hinT"
                     /locus_tag="JN331_000716"
     CDS             complement(6505..6864)
                     /gene="hinT"
                     /locus_tag="JN331_000716"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY7258939.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7204..9393
                     /gene="fhuE"
                     /locus_tag="JN331_000717"
     CDS             7204..9393
                     /gene="fhuE"
                     /locus_tag="JN331_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HAY7258940.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSSIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDHVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(9453..10886)
                     /gene="ptsG"
                     /locus_tag="JN331_000718"
     CDS             complement(9453..10886)
                     /gene="ptsG"
                     /locus_tag="JN331_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY7258941.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11181..11978)
                     /locus_tag="JN331_000719"
     CDS             complement(11181..11978)
                     /locus_tag="JN331_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7258942.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(11989..12993)
                     /gene="holB"
                     /locus_tag="JN331_000720"
     CDS             complement(11989..12993)
                     /gene="holB"
                     /locus_tag="JN331_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY7258943.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(12990..13631)
                     /locus_tag="JN331_000721"
     CDS             complement(12990..13631)
                     /locus_tag="JN331_000721"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY7258944.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13621..14643)
                     /gene="yceG"
                     /locus_tag="JN331_000722"
     CDS             complement(13621..14643)
                     /gene="yceG"
                     /locus_tag="JN331_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY7258945.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14646..15455)
                     /gene="pabC"
                     /locus_tag="JN331_000723"
     CDS             complement(14646..15455)
                     /gene="pabC"
                     /locus_tag="JN331_000723"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY7258946.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISSDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(15575..16816)
                     /gene="fabF"
                     /locus_tag="JN331_000724"
     CDS             complement(15575..16816)
                     /gene="fabF"
                     /locus_tag="JN331_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY7258947.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(16903..17139)
                     /gene="acpP"
                     /locus_tag="JN331_000725"
     CDS             complement(16903..17139)
                     /gene="acpP"
                     /locus_tag="JN331_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7258948.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17350..18084)
                     /gene="fabG"
                     /locus_tag="JN331_000726"
     CDS             complement(17350..18084)
                     /gene="fabG"
                     /locus_tag="JN331_000726"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY7258949.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18097..19026)
                     /gene="fabD"
                     /locus_tag="JN331_000727"
     CDS             complement(18097..19026)
                     /gene="fabD"
                     /locus_tag="JN331_000727"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY7258950.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALDYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19042..19995)
                     /locus_tag="JN331_000728"
     CDS             complement(19042..19995)
                     /locus_tag="JN331_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY7258951.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20063..21133)
                     /gene="plsX"
                     /locus_tag="JN331_000729"
     CDS             complement(20063..21133)
                     /gene="plsX"
                     /locus_tag="JN331_000729"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY7258952.1"
                     /translation="MKRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21214..21387)
                     /gene="rpmF"
                     /locus_tag="JN331_000730"
     CDS             complement(21214..21387)
                     /gene="rpmF"
                     /locus_tag="JN331_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY7258953.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21439..21960)
                     /gene="yceD"
                     /locus_tag="JN331_000731"
     CDS             complement(21439..21960)
                     /gene="yceD"
                     /locus_tag="JN331_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY7258954.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22159..22743
                     /gene="yceF"
                     /locus_tag="JN331_000732"
     CDS             22159..22743
                     /gene="yceF"
                     /locus_tag="JN331_000732"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY7258955.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22855..23814)
                     /gene="rluC"
                     /locus_tag="JN331_000733"
     CDS             complement(22855..23814)
                     /gene="rluC"
                     /locus_tag="JN331_000733"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY7258956.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24387..27572
                     /gene="rne"
                     /locus_tag="JN331_000734"
     CDS             24387..27572
                     /gene="rne"
                     /locus_tag="JN331_000734"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY7258957.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27769..28722)
                     /gene="flgL"
                     /locus_tag="JN331_000735"
                     /pseudo
     CDS             complement(27769..28722)
                     /gene="flgL"
                     /locus_tag="JN331_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
     gene            complement(28734..30376)
                     /gene="flgK"
                     /locus_tag="JN331_000736"
                     /pseudo
     CDS             complement(28734..30376)
                     /gene="flgK"
                     /locus_tag="JN331_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(30442..31383)
                     /gene="flgJ"
                     /locus_tag="JN331_000737"
     CDS             complement(30442..31383)
                     /gene="flgJ"
                     /locus_tag="JN331_000737"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY7258958.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKSSEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31383..32480)
                     /locus_tag="JN331_000738"
     CDS             complement(31383..32480)
                     /locus_tag="JN331_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY7258959.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32492..33190)
                     /gene="flgH"
                     /locus_tag="JN331_000739"
     CDS             complement(32492..33190)
                     /gene="flgH"
                     /locus_tag="JN331_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY7258960.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANVSRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33243..34025)
                     /gene="flgG"
                     /locus_tag="JN331_000740"
     CDS             complement(33243..34025)
                     /gene="flgG"
                     /locus_tag="JN331_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY7258961.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKVQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34163..34914)
                     /locus_tag="JN331_000741"
                     /pseudo
     CDS             complement(34163..34914)
                     /locus_tag="JN331_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(34934..36862)
                     /gene="flgD"
                     /locus_tag="JN331_000742"
                     /pseudo
     CDS             complement(34934..36862)
                     /gene="flgD"
                     /locus_tag="JN331_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(36874..37278)
                     /gene="flgC"
                     /locus_tag="JN331_000743"
     CDS             complement(36874..37278)
                     /gene="flgC"
                     /locus_tag="JN331_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAY7258962.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSAPRSYQANVEVLNTVKSMVLKTLTLGQ"
     gene            complement(37282..37698)
                     /gene="flgB"
                     /locus_tag="JN331_000744"
     CDS             complement(37282..37698)
                     /gene="flgB"
                     /locus_tag="JN331_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAY7258963.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37853..38512
                     /gene="flgA"
                     /locus_tag="JN331_000745"
     CDS             37853..38512
                     /gene="flgA"
                     /locus_tag="JN331_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY7258964.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38588..38881
                     /gene="flgM"
                     /locus_tag="JN331_000746"
     CDS             38588..38881
                     /gene="flgM"
                     /locus_tag="JN331_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY7258965.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            38886..39302
                     /gene="flgN"
                     /locus_tag="JN331_000747"
     CDS             38886..39302
                     /gene="flgN"
                     /locus_tag="JN331_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY7258966.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMRQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(39342..40877)
                     /gene="murJ"
                     /locus_tag="JN331_000748"
     CDS             complement(39342..40877)
                     /gene="murJ"
                     /locus_tag="JN331_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY7258967.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(40987..41910)
                     /locus_tag="JN331_000749"
     CDS             complement(40987..41910)
                     /locus_tag="JN331_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7258968.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(41912..42559)
                     /locus_tag="JN331_000750"
     CDS             complement(41912..42559)
                     /locus_tag="JN331_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY7258969.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNVVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42570..43154)
                     /gene="rimJ"
                     /locus_tag="JN331_000751"
     CDS             complement(42570..43154)
                     /gene="rimJ"
                     /locus_tag="JN331_000751"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7258970.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43390..44598
                     /gene="mdtH"
                     /locus_tag="JN331_000752"
     CDS             43390..44598
                     /gene="mdtH"
                     /locus_tag="JN331_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY7258971.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44662..45309
                     /gene="grxB"
                     /locus_tag="JN331_000753"
     CDS             44662..45309
                     /gene="grxB"
                     /locus_tag="JN331_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY7258972.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45443..46003
                     /locus_tag="JN331_000754"
     CDS             45443..46003
                     /locus_tag="JN331_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7258973.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46109..47155
                     /gene="pyrC"
                     /locus_tag="JN331_000755"
     CDS             46109..47155
                     /gene="pyrC"
                     /locus_tag="JN331_000755"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY7258974.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        11415 a        12791 c        11757 g        11312 t
ORIGIN      
        1 tgaagcgact tctcttgcgg acctcgtcga agcgaccgga gccaaagcgc ccacgctata
       61 cgcggaattt accaacaaag aggggttatt tcgtgccgtt ctcgaccgct atatcgatcg
      121 ttttgccgct aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg agtctgcgct
      181 ggctgactat tttgctgcca tcgccaactg ctttaccagc aaagacaccc cggcaggctg
      241 cttcatgatc aacaactgca ccaccctctc cccagattca ggagatatcg ccaatacgtt
      301 gaaatcacgc catgcgatgc aagagcgcac tttgcagcag tttttatgtc aacgacaagc
      361 gcgcggggaa atcccgaccc actgtgacgt gacacatctg gcagaattcc ttaattgtat
      421 tattcagggg atgtcgatca gcgcacgcga aggtgcatcg ctggaaaaac tgatgcagat
      481 tgccggaacg actttgcgtt tatggcccga actggtgaaa taagtcgcaa aaaaagcccc
      541 tcagcctgag cgaatgaggg gccaaattgc agagtacagc cttgttatac tttgttattc
      601 aaaatcagtt gcccgttact atctagcggg atctgcgtac ccggatcgcg gtccatgcgg
      661 attttgccct gctgatcgcc aatcttatag gtcacatcat aaccgagcat tttttctgac
      721 ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct ttcctggaga
      781 gagccctgga tctggttacc ggcatatcca ccacccagcg cccccacaac agtggcgaca
      841 tctttaccgc gaccaccacc aaactgatgc cctatcacgc cacctgcaac agcgccgagc
      901 accgacccgg taatgcgatt ttcatcctgc accggtcgac gatgggtcac tgtgacgttg
      961 cgacactcct gacgcggtgt tttaaccgtt tccttgattg gggttgcaga aacaacctga
     1021 gcgtattgcg ggccccgttc aaacacgttc agactggcca ctgccgctac gcccaacgca
     1081 gctgcgacac caatcccgat acccgccaac attgatttat tcacgggaac acctccttct
     1141 ttgcagttat gccaatcatg caagcgaagg agaaaagcag ctatcagaca gaagatgaaa
     1201 agcgcgggaa tgactggaaa tacttaagga ttcggaggag tctgaaggaa tgcctgatgc
     1261 gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat ttgtccaggg cggataaggc
     1321 gtttatgcca catccgccag tgtacgtcga ttaatgcaac ttcaaacgcg gacggataac
     1381 gcggttaata ctccccacca gcatcattaa tccggtttta aagtaaccat gcagcgcaat
     1441 ctgatgcatt cggtatagcg agatatatac aaagcgcgca attcgtcctt caatcatcat
     1501 tgagccgcgc gtcaggttac ccatcaggct accaacggtg gagaagttcg acagcgatac
     1561 cagcgaacca tgatctttat actgataatt tttcagcggc ttaccgttca tctgcgccag
     1621 aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc tgagcacgcg gcggaacaaa
     1681 gcccccttcc ggacgcgggc atgacgcgca gtcgccaata gcgtaaatgt ctggatcgcg
     1741 agtggtttgc agcgtcggtt ccaccaccag ctggttgata cggttagttt caagaccacc
     1801 gatatctttc aggaagtctg gcgctttgat cccggctgcc cataccatca gatcagcctc
     1861 aatatattcg ccatctttag tgtgcaggcc gccttcatca gcactggtga ccatggtttg
     1921 cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg gccgcagcag agatacgtgg
     1981 cggtaatgca ggcaaaatac gttctcccgc ttctaccagc gttacgttca gggcttcgtt
     2041 ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgacc gcgttgtgca attcagcgga
     2101 gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg ttcactttgc catttgcgcc
     2161 caggttggca gagtatttca ggaacagatt cagcatctcc tggtggaagc gacgcgcctg
     2221 gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct ggcgtattga aatcgttaga
     2281 ggtgctaccc agcgccatga ccagggtgtc ataggcgatt ttacgttccg gaaccagcag
     2341 ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt ttcgcttcac gatcaatatc
     2401 aatgacggaa cccaactgga actggaaacc atgattgcgc gcatgggcca gatagctcaa
     2461 cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg tgcagcagcg gtttccacag
     2521 gtggctgtgg ttacgatcga ccagcgtaat tttggctttt ttcttacgtc ccagcttatg
     2581 ccccagctgt gttgccattt ccagcccacc agcaccgccg ccgacaatca caatcttttt
     2641 caatggcgta gtcaacgtga cccccttaaa tttattaacc aattgttaat taatggttat
     2701 taacatagct cttaattaac aacaggttac agcaatgaaa atgttcttcg ctattgagaa
     2761 taacatgaat ggtgcattgg tcataccaaa attgatgtta gtcaagtttt gttgcttaaa
     2821 agataggcaa ctttaccggg tgagatcaaa aaatagagtt tattgtttat tatttgagca
     2881 aattcggaag agtaatatag ggtgaagcgt atatcgaggg cgtttggtcg tcctgataag
     2941 atgcgcagca tcgcatcagg cattgatgct cgactgtcgg atgcggcgtg gacgccttat
     3001 ccgacttaca gaattcggag cgcatcaggt accggcaagg ttacctgatg cgacgagggc
     3061 atttacccga gggttttgaa cgctttaatg cgctgtaaat gcggggagat attcttgaat
     3121 ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat gatgcaactc ttcagatgtc
     3181 cgctggctgt taagtgcttc atcattacgc gacaaaataa ccagacaacg atcgcgattc
     3241 ttctcgcgga agttggtcac acacttagtg gcaatatcgg catactcttc cgggcgatca
     3301 atcttgcctt ccatgttctc ataagggaac aaattagggt tgaagatcac ctggcggata
     3361 tcgcaaagaa aaccaatccg ttccgcccag tatccgccca ggccaacgcc gcaaattagc
     3421 ggacgctcgt caacgttcag ttgcaacatt ttgtccactt ctttaagcag atgctgcata
     3481 tcatgtttcg gatgccgcgt actgtagctt atcaagcgta catccgggtc aataaactgc
     3541 aattgtaaga ctttctcgtg gttacccgga ctgttagagt caaaaccgtg taaatagata
     3601 atcatcgctt cctcacataa gccagggtta gtgacctgct ttctcttcct gccagcgttc
     3661 atgtaactga ttcagacggg tgctgatcgc tttccagcga gccgagtcca tcagttcctg
     3721 tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt tctgccttga tcggtgacag
     3781 attatctaac acgctgacgg cccctttacg attattgcag accaggatca tatcgcaacc
     3841 cgcatccagt gatgcctgcc cgcgttcggc ataactgccc ataatcgcgg caccttccat
     3901 cgataaatcg tcagagaaaa tcacgccgtc aaaacccagt tcctgacgca aaacggtttt
     3961 cagccagtag ggagaaccgc tcgccggacg cggatcaaca tcactgtaga tcacatgcgc
     4021 aggcataatg gcgtcgagtt tattttcgcg gattaaggaa ctgaagaccg acatatcttt
     4081 agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt tctttgtgtg agtctgccgt
     4141 tactgcaccg tgtcctggga agtgtttccc ggtcgttttc attccggctt catgcatacc
     4201 atcaataaac cggctggcaa ttgccagggc tttttgtgga tcggcatgat aagaacgctc
     4261 gccaatcgcc gcgctgatat gcccggcatc cagcactggc gcaaagctga tatcaatatc
     4321 catagcgatc atttcactgg ccatcagcca acccgcctct tgcgccagtt tgccgccctc
     4381 ttccattccc aacagcgcag caaaggattg tgctgccggt aagcgggtaa aaccttcgcg
     4441 aaaacgctgc acgcgtccac cttcctgatc taccgccacc accagatgat tgcgcgatgc
     4501 tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga tcatgatagt tacgcgtaaa
     4561 gagaatcagc cctcccacca gcggatgcgc cagtatttca cgctcttccg cgtccagttc
     4621 gtaaccttcg acatccaaca ttactggacc cacactgctc tccttattgt tttattaata
     4681 gctgccgcca ggtgtcatcg gccagcctga taaattgttg atcgccggtt tgtcgccagc
     4741 ggtactcaaa ccaccctcct ttgagcatca gcagccaggg aaaccatcgc ctgacctgac
     4801 gccataattg cgccggataa atcttcgcgc gagtggcata gtcattgacc aattgccggt
     4861 gctgttcagt attttccacc cacaccgccg ccagttccag cgcgatatca ccatctccgg
     4921 catactccca gtcgatgagt tttaaccctg atgcgctatg cactaaattt ccggcgtgga
     4981 catccatatg caatggactt aagcgtaaag gtcgtggttc ccgcgctttg cgcagacgtt
     5041 ttaacattcg cagccaaccc acagtccgcc gcgccggatc gctttgctgc cagtacagtt
     5101 ccagtaaggg caacagcgtt attcgccagc caaaacgtgg ttgttgatgt agataataca
     5161 gcaagcctgc cagttcgttg gtatccggca aatacgtttt taccgcgccg ggcagatagt
     5221 cgactaccat ccagtcacgg agatataaat gcggcttcgg tgcaatgctt gcgggtagtt
     5281 gtgataaagc ccgatactgg cgcaagaacg cggactgcgg cgcatcagga tcgtgcggct
     5341 gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa actaccgcca ctaagcccac
     5401 tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg cgacagcaat tcgtcgcgcg
     5461 tgatgggatt attgctgcga aacggcacct ttacctgacc agataatttc gcccgtctgc
     5521 accagcatca gctgcatttg tagggtcgga gcgttaacgt tgccagaggc gctggagtac
     5581 agcacgtaat gagcgccgac attgcgggca atgcctatgg ctttactacg ggtgcctaaa
     5641 ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg acagttgctg gacggaaacc
     5701 agggtaaatt tcccgttatt agccagcgca tttcgcagcg tttcggtcgc ttctgcggca
     5761 ttcagcgaac cgttagtacg gttgttaacg ctatcaacca gcaggacgct acccgcagtc
     5821 accccgtcag ccccaagcat cttactgacc atcggctgca ttgcgccatt ccagtcataa
     5881 tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt gctcaattgg gcctagctgc
     5941 tgcgggatcg tcggcaccga gggcactgtg gggacaggct gttgtggctc ggctggttgt
     6001 tccggcgctg gtttcacttc ttctaccggt gcaggttcac gttgccccac acacccggcg
     6061 agaaacatcg ccagcgcggt aatcaaggcg tagcgactca tttttgtcat caagattcac
     6121 ccctttacaa atatagataa agtctgactt tgtgcgcccc cagaaaattg gcgctgccgt
     6181 acagcgttac cgccgaatgt gcgggaatgg taacgctgcg tggcctttcc agaggatgca
     6241 tctccagccc tctggcgtca taccagtaaa aacgataatg aacggtaacg ggttcttgcc
     6301 tttcgttata aagcgttgag gatgctgaag gttgaatatc agacgtcgaa aggacgggct
     6361 tttctgcact gatgcccgca gccaataaag atgactccat caccagcgat tgctcatcat
     6421 tcaccggaat ttccggatgt gaacgacagc ccaccagcag taacaacacc agagacacca
     6481 gcccaaagca tccttttctc atcgttacag acctttatgc gccagcattg gtcccagcgg
     6541 acggccaccc aacaagtgca tatggatgtg gtaaacctct tgtccgccat ggcggttggt
     6601 attcatgatc agacgatagc catcttcggc aataccttct tgctcagcaa tttttgccgc
     6661 tacggtgatc atgcgtccca gcgcctgctc atgctcagct gacacgtcgt tcacagtcgg
     6721 aatgaggata ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa tatcgcgaaa
     6781 cgccgttacc agatcatcct ggtagacgat atcggagggg atctcacgac gaataatttt
     6841 gctgaatata gtttcttctg ccacgacatt ttcctttttc aaatagccat gcgttgcgcc
     6901 atgctacgcc gcgccacact gcgagtatag agtatggtcg agttaaacct gctctttcaa
     6961 ccttaactca cgatttctta agcaaaaaat cgttttgctg ccgtatatat cgccattatt
     7021 cccatttctg cctgcgaatg cgctgaaaag acattctctg tttggtttac acgacaaata
     7081 tgaatgcgta tatttctcat ttgcatttgc atttgcaagc aaaattattc gcacactaaa
     7141 tataaccgct catcaccctc ctccggcatg agcctgacga caacataaac caagagattt
     7201 caaatgcttt caacacaatt taacagggat aatcaatatc aagccatcac caaaccgtca
     7261 ctacttgccg gttgcatagc actggcacta ttaccttctg ccgcttttgc tgcaccagcc
     7321 actgaagaaa cggtgattgt tgagggttca gccacagctc cagatgatgg cgaaaatgat
     7381 tacagcgtaa cgtctacctc tgcgggtacc aaaatgcaga tgactcaacg tgatattcct
     7441 cagtcggtca ctattgttag ccagcagcgg atggaagatc agcagttaca aacgctgggc
     7501 gaagtgatgg aaaacacgct ggggatcagc aaaagtcagg cggattccga tcgtgctctt
     7561 tattattccc gcggattcca gatcgataac tatatggttg atggtatccc cacctatttt
     7621 gaatcgcgct ggaatctggg cgacgcactt tctgatatgg cactgtttga acgcgtagaa
     7681 gtagtgcgtg gcgcgacagg actcatgacc gggacgggta atccatctgc ggcaattaat
     7741 atggttcgaa aacacgcgac cagtcgtgaa tttaaaggcg atgtctcggc ggaatacggt
     7801 agctggaaca aagaacggta tgtggctgat ttacaaagcc cactcaccga agacggtaaa
     7861 atccgcgcgc gaattgtcgg cggctaccag aataacgact catggctgga ccgctacaat
     7921 agtgaaaaga ccttcttctc gagcattgtc gatgctgatt taggcgatct tacgacgctt
     7981 tcagccggtt acgaatatca gcgcattgat gttaatagcc caacctgggg cggtttaccg
     8041 cgctggaata ctgatggcag cagcaacagt tacgatcgcg cacgcagtac cgcacctgac
     8101 tgggcgtaca acgataaaga gatcaacaag gtctttatga ccctgaagca gcggtttgct
     8161 gatacctggc aagcgacact gaatgccacc cactctgaag tcgaatttga cagcaaaatg
     8221 atgtatgtcg atgcctatgt aaacaaagcg gatggtatgc tggttgggcc atacagtaat
     8281 tatggacctg gctttgatca tgtcggcggc accggttgga acagtggcaa acgtaaagtt
     8341 gatgcgctgg atttgttcgc tgacggtagt tatgaattgt ttggtcgtca gcacaatcta
     8401 atgtttggtg gcagttacag caaacaaaac aatcgttact tcagttcatg ggccaacatc
     8461 ttcccggatg aaattggcag tttctacaac tttaatggca atttcccaca aaccgactgg
     8521 tcaccacaga gcctggcgca ggacgatacc acacatatga aatcgttata tgctgccact
     8581 cgtgtcaccc ttgccgatcc gctgcatctg atcctcggcg cacgttatac caactggcgg
     8641 gttgatacgc tgacttacag catggagaaa aaccacacca cgccttacgc tggtctggtg
     8701 tttgacatca atgacaactg gtcgacctac gccagctata cctctatttt ccagccgcaa
     8761 aatgatcgtg acagttcagg caaatatctg gctccaatca ccggtaacaa ctacgagctg
     8821 ggtctgaaat cggactggat gaatagccgt ctgaccacca cgttagccat cttccgtatt
     8881 gagcaggata atgtcgctca gtccaccggt acacctatcc ccggcagcaa cggcgaaacc
     8941 gcctataaag cggtggatgg gacagtcagt aaaggggtgg aatttgaact caacggcgca
     9001 attaccgaca actggcagct gacatttggc gcaacgcgct atattgcaga ggataacgaa
     9061 ggaaacgccg ttaatcctaa tctgccacgc accacggtta aaatgttcac cagctatcgg
     9121 ttgcctgtca tgccagagtt gacagtcggc ggtggtgtta actggcaaaa tcgcgtgtat
     9181 accgacaccg tgacaccgta tggcaccttc cgcgccgagc aaggtagcta cgcgctggtg
     9241 gatctcttca cccgctacca ggtgacgaaa aacttctcgt tacaggggaa cgtcaataac
     9301 ctgttcgaca aaacctacga taccaacatg gaaggttcta tcgtctacgg cgcaccgcgt
     9361 aatttcagca ttaccggcac gtatcaattc tgaataacac ctgtaaaaaa ggcagccatc
     9421 tggctgcctt agtctcccca acgtcttacg gattagtggt tacggatgta ctcatccatc
     9481 tcggttttca ggttatcgga tttagtaccg aaaatcgcct gaacaccaga accagcaacc
     9541 actacgcccg ctgcgcccag tttcttcagg ccagcctgat ccactttaga cacatcagca
     9601 acgctgacgc gcagacgggt aatacatgcg tcgaggttag taatgttttc tttaccacca
     9661 aatgcagcaa ccagagccgg tgccatttcg ctggtacctg ttgcttttgc atcttcagtc
     9721 gcgtcttcac gacccggcgt tttcagatcc agtgctttaa tcagcacgcg gaagatggtg
     9781 tagtaaacaa tcgcataacc gataccgacg atcgggaaca gccacagttt gctgctgtta
     9841 ccagacagaa cgatgaagtc gatcagaccg tgcgagaacg acgtaccgtc acgcatcccc
     9901 agaagaatac agattgggaa tgccaggcct gccagaatcg cgtggatgat gtacaggatc
     9961 ggcgcaacga acatgaagaa gaactcgatc ggctcggtga taccggtcag gaacgaggtc
    10021 agcgccgcgg agatcataat accgcccact ttcgcgcggt tttctggttt agcagagtgc
    10081 cagatagcaa ttgcggcagc tggcagaccg tacattttga acaggaaacc accagacagt
    10141 ttacccgcag tcgggtcacc cgccatataa cgcggaatgt cgccgtggaa aacctgacct
    10201 gctgcgttgg tgtattcacc aatctgcatc tggaaaggta cgttccagat gtggtgcaga
    10261 ccaaacggta ccaggcaacg ttcgatgaaa ccgtaaatgc caaacgcaac taccgggttc
    10321 tggtaagcag cccactgaga gaaggtctgg attgcagaac caatcggcgg ccaaatgaag
    10381 gacagcacaa cgccagtaaa gatggcagcc aggccagaaa tgatcggcac aaagcgttta
    10441 ccggcaaaga agccaagata ctcaggcagc ttaatacggt agaaacggtt aaacatgtac
    10501 gctgcgatcg caccggagat aatccctccg agtacgccag tatccgccaa gtgtttagag
    10561 gcgatttctt cagcaggtaa atgcagtacc agtggcgcaa ccacggccat ggttttaacc
    10621 atgatgccat aggcaacaac tgcggccagc gcggatacgc catcgttatt ggtaaagccg
    10681 agggcgacac cgatcgcaaa aatcagtggc atgtttgcaa agacggaacc gcctgcttct
    10741 gccataacat gcgatacaac ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc
    10801 agaatacctg cgataggtag tacggatacc ggcagcatca gcgatttacc gaccttttgc
    10861 aggttagcaa atgcattctt aaacataatt gagagtgctc ctgagtatgg gtgctttttt
    10921 tacgttctca cgcgtggcaa ggggggagag cctcgccgtg tacagggcat ctaagcgccc
    10981 tttatttatt acacagagta aaataattca gtgccaattt gtttgacggc tatcacgttt
    11041 caacttcaga tcattggaga agttgcacat tttgacaaaa catatgtcac aatatttcaa
    11101 acgcagttat tcaccgccca attttcggcg gtgaacttta ctcgttttag ccattaatta
    11161 cgagctttaa aaaaactcat tcaacggatg gattgaaggc gggaagcgtc gatgtgaaac
    11221 agacgggcga agttatcggt ggttacctgc gccagttctt caacggcaac acctttcaac
    11281 acagccatgt attctgcaac gtcacgaacc atcgcaggtt gattctcttt tcctcgatgc
    11341 ggtaccggcg caaggtaagg tgagtcagtt tccaccagta atcgatccag ggggacataa
    11401 cgcgcagcat cgcgcagttg ctccgcatta cggaaggtca caatgccgga aaaggagatg
    11461 taaaatccga gatccagtaa tttacccgcc gtttctctgt cctctgtaaa acagtgtagt
    11521 acgccaccgc aatccgtcac tttttcttcg cgcagaattg ccagcgtatc ggcacgggcg
    11581 tcacgggtat ggacgataac cggcttgttc agttcacgac caatctggat atgatggatg
    11641 aaggactctt gctgacgtac tttagtttcc ggtgtgtaat aataatccag cccggtttca
    11701 cccagcgcta caacaccctc ttctgccgcc agacggcgta aatcttctac atcgtaggga
    11761 tcattctggt tgagtggatg cacgccacaa gaaaatacga cgttgtcacg ttcgcctacc
    11821 agatcccgca tatgtaaata acccggtaat gttgtggcga ctgccagaca aaatttcaca
    11881 tcgcgtgcgg cggctttcgc cagaacgtca tccacgtcct tatgcaaaga ttcataatcc
    11941 agaccatcga gatggcagtg tgagtcgact aaaaacatga tgtctctctt aaagatgagg
    12001 aaccggtagc acaacgcccg gttgcaggta atgctcaata cgcagtaaaa gatcggtgat
    12061 gagaagctcg cggttgatgc ctgtgacaga cattaactgt tcacgaatgt ggcaaacatc
    12121 ccccagtata gcctgcaggc gcgagggaga aagatggttt gccagttcgg cgaccaggcc
    12181 cggcacatca acattggtca cctgcgcagc gccatgatgg cgttttagcg catccatcag
    12241 caacgttgcc agccagtgta aacgcgccgg agcttgttca tgattaaggg ccgccagcag
    12301 cgaataccag tcgcccgatg gcacgctata tgccaacgcc tgacacaatg tttcacgagc
    12361 ctgccagtta tctccctgaa acaacgccag tgccgcgcta ggcgaaccgg cgcttaagcg
    12421 caatgcggca agtaatgcat cctgtgacat tgtcacttcg cgtgaaagcc aagtcacggc
    12481 gtactgttct ggaggcggcg caaggtaatg taaccgacaa cgactacgta atgttgccag
    12541 taaacgttca ggctcgcggg tagccaggaa aaaccaagtt tctgctggtg gctcttcaag
    12601 cgttttcagc aatgcgttag ccgcggcgtc ggtcagtaag gcagcatcgg ttacccagac
    12661 aactttcgca ccacctaggc gtgcgtgctc attcagcttt tcggtgacct cacgtaccgc
    12721 atcaatgccc agcgtatttt tccctttttc gggagccagg gtgtagtaat cgggatgcgt
    12781 gccagcctgc atcaactgac atccacgaca gtgaccgcaa cttttgtggc cctgcggttg
    12841 ttggcagagt aaataacggc tcagggcgta gattaaagca tcatcgccca tgcccggtaa
    12901 cgcctgaatg agtagcgcat ggtgacctct tccggcctga tagctggcta ccagtttttc
    12961 gaaatcaggt cgtaaccatg gataccatct catgcgtcca actccttcac ccagtgggtc
    13021 acggtagtgc ggattgcatc catcacggcc tccagcggct gggtggcatc aatggtatga
    13081 atacttttat cttgtgcagc cagttccaga tagcgggcgc gggtgcgatt aaagaaatcg
    13141 aaagactctt gctcaatacg atccagctcg ccgcgcgcac gcgcgcgttt taagccaact
    13201 tccggggtaa catcgagata gagcgttaag tcggggcgaa aatccccgag aacagcatca
    13261 cgcagtgttg ccagcatatg ttggtcaata ccacgtcctc cgccctgata cgcctgagtg
    13321 gagagatcgt ggcgatcgcc aatcacccag gtgccgttag ccagcgcagg tttgatgacc
    13381 gtttctacca gttgaacgcg cgcggcataa aacatcagaa cttcggcttt atcggtaatg
    13441 acttcatcgc ctaccgattt gatatccagc accaggcttc ttaacttttc ggcaagttgc
    13501 gtaccgccag gttcccgagt gaaaaccatg tcgcggatac ccagttgctc gagcgtctca
    13561 accaccacat tacgcgcggt agttttgcct gcgccttcca gcccctcaat gacgatatac
    13621 ttactgcgca tttttttcct taagcacttt cagataatcc tgcacagact tgttatgact
    13681 ggcaagattg gtattaaacg tgtgaccacc tttaccatcg gccacaaaat agagatacgg
    13741 cgtttttgcc ggatgcgcag cagccttcag cgaatccgcc cccggcgtag ctatcgcacc
    13801 cggcggcaaa ccggtaatgg tataggtgtt atacgctgtc ggcgtttcca ggtctgcacg
    13861 agaaagtttg ccattataac gctctcccat cccgtaaatc acggttgggt cggtctgcag
    13921 gcgcatacca atgcgtaaac ggttgataaa taccgaggca acctgatcgc gttcactggc
    13981 aacggcggtt tctttttcga taattgatgc catcgtcacc agctgatttt tatctttata
    14041 aggcagaccg tccgcacgcc cttcccaggc gctatcgacc gctttcacca ttttcttgtg
    14101 agctcgcttg agtaacgcga catcggtggt attggcggta tacatcaagg tgtctggcca
    14161 gaaccaacct tcaatccact ccgggttttc cagttcaagt gcctgcgcta cggtggcgta
    14221 cttatcgtca ctcagcgtgt gcttgatata cggagcctca cgcaattgct tgaggtagtc
    14281 gctcagacgc atcccttcta ccagtcgcag cgggaactgt gcttctttac cgctttccaa
    14341 caatttcagc atctcgcgca cggtcatctg cggtgtaaag cggtaagtcc cggctttaaa
    14401 gtgagaaaga tccggttcga tacgcagcag ccattgaaaa acccgtgggc gattgatgat
    14461 cttatcagca taaagctgtt caccgagcgc cagacgtccg gtccctggct tcagggtaaa
    14521 tatcgtctct tctttgataa gcaatttgct gtcggcaaga tggcgaacct tccagacgcc
    14581 cacaccagcg gcgataccca gtaccaccag caataacaag ataatcaata acactttttt
    14641 catgactaat tcgggcgctc acaaagtggg gctaaatatt catataacgt tgctgacgaa
    14701 aaggtgacat cgccacaggc acgtacgggc atcactggca ttaacgcatt acaaataacc
    14761 atctcatctg cctgcaacgc ctcctccagc gaggcttgca cttcgacaag ctgataagag
    14821 gattgtgcca gtaaacggat acagaattgt cgcataatgc cgttaacacc tgcctgatcc
    14881 agtcgcggcg tataaactac attgcccttc cgccagaaca aattagccgc acagcattcc
    14941 gtaacccacc cttcgctgtc aaggaccagc gcctcatcag cgtttgtctg ctcaagatga
    15001 gagcgaatca atacttgctc aagccgatta agatgtttaa tacctgcaag atgaggattg
    15061 cgccccagcc gcaccgggct tagcgccaac gtcatccctt cgttacgcaa acggtcgtaa
    15121 tgtgcaggat aagccgtaac ggagagaatc cgcgttgctg gtccgctgtt caatgtgctg
    15181 taccctcgcc cgccactacc gcgactgatc acgactttca gcacaccatt ttgctgttct
    15241 gctgccagcg ttttcatctc ttgttcaagc tgaggccaga agtcactgga aatcatcaac
    15301 cgctgacaag catcctgtag tcgctggata tgcgccgata acaaactgac tttaccgtcg
    15361 ataactctgg cggtggtaaa acaaccatca ccaaactgcg ttgcccgatc gcttactgcc
    15421 agcgattcct gcttataacc gttaattaag aacataccgg ctccttatca gagaattgtt
    15481 agtgtggcag catgttcact acggaacaag tcggaataaa agctaagaaa aaaggcccgc
    15541 aagcggacct tttatatggg tggaaaatga caacttagat ctttttaaag atcaaagaac
    15601 cattagtgcc accgaagccg aaggagttac acagcgtgta ttccattccg ctgacctgac
    15661 gtgcttcatg cggtacgaaa tccagatcgc aaccttcatc cgggttatcc aggttgatgg
    15721 ttggcggaac agcctgatcg cgcagcgcca ggatggagta gatagattct actgcacccg
    15781 ccgcacctaa caggtgaccg gtcatagatt tcgtggagct taccaacaca cggcttgcag
    15841 cttcaccgaa gatggttttc accgcctgcg cttcagcttt atcgccagcc ggcgtagaag
    15901 taccgtgcgc gttaacgtag ccaatctgac tcgcttcaat gcctgcatca cgcagagcat
    15961 ttgccattgc cagagctgcg cctgcgccat tttctggcgg tgacgtcata tgataagcat
    16021 cgctgctcat accaaagccg acgagttcag cgtaaatttt cgcaccgcgt tttttcgcgt
    16081 gttcgtactc ttcaagtacc agcataccgg caccatcgcc cagtacgaag ccatcacgct
    16141 ctttatccca cgggcggctc gccgcttgcg ggttatcatt gcgggtagat aatgcacgtg
    16201 ccgcgccaaa accaccaacg cccagcggcg tactggcttt ctctgcgcca cctgcaacca
    16261 tcacgtcagc atcgccatac gcgataatac gcgcagcatg gccaatgttg tgcacgccgg
    16321 aagtacaggc ggtcgcgata gagatgcttg ggccacgcag gccatacatg atagtcagat
    16381 gacctgccac catgttcaca atcgttgacg gaacgaagaa tgggctgatc ttacgtggac
    16441 caccgttcat cagagatgtg tggttttctt cgatcagtcc gaggccgcca atcccggagc
    16501 caattgcggc accaatgcgg gttgcgttct cttccgttat ttcaaggcca gaatcctgca
    16561 tggcctgaac gccagcgaca attccatatt gaatgaaggc atccatcttg cgctgttctt
    16621 tgcgcgagat aatgtcctca cagttaaaat cctttactaa gccagcaaat ttcgttgcat
    16681 aggcgctagt atcgaaatgg tcgattaggc tgatgccact ctgaccggca agcagagctt
    16741 tccaggtaga ctctacggta ttgccgacag gagacaacat gcccagtccg gtcacaacta
    16801 cacgacgctt agacacgttt gtcctccagg gagggaaaaa tgattctagt gggacaaaaa
    16861 gataaaactc aggcggtcga acgaccgcct ggagatgttc acttacgcct ggtggccgtt
    16921 gatgtaatca atggcagcct gaacggtggt gattttctca gcttcttcgt ccggaatctc
    16981 agtatcaaac tcttcttcca gagccattac cagctcaacg gtgtcaagag aatccgcgcc
    17041 taggtcttca acgaaagaag cattgttggt aacttcttcc tgcttaacgc ccagctgttc
    17101 gccgataatt ttcttaacgc gttcttcgat agtgctcata ctcttaaatt tcctatcaaa
    17161 actcgctttc gcgatggttt tcgtagtgta taaaatgttg aaaaatttgc aactaaatcc
    17221 cggcaggtct taccacgatt ttacgttatt ttgaggcctt cccccccaat aacgcaaatc
    17281 attttgcact aattattact cataaccacg cagactgcgc gcaacatgag cattttgtgc
    17341 aaatcgcggt cagaccatgt acatcccgcc gttcacatgc aaagtttcac ccgtgatgta
    17401 agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg atttcctgtg cgccgccgag
    17461 gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg tcatcgctca gcgcacgtgt
    17521 catgtccgtt tcaataaagc ccggagcaac aacgtttaca gtaataccgc gtgacgcaac
    17581 ttcgcgcgcc agtgatttac tgaagccgat caagcccgct ttcgccgcag cgtagttggc
    17641 ctgaccgcca tttcccatgg taccaaccac agaaccgata gtgataatac gaccatgacg
    17701 ctttttcatc atagcgcgca ttaccgcttt tgacagacgg aaaacagatg aaaggttagt
    17761 ttcgataata tcgttccact cttcatcttt cattcgcatt aacaggttat cacgagtgat
    17821 accggcatta ttgaccagga tatccacttc accaaattct gcgcgaattt tttccagaac
    17881 agattcgata gatgccgggt cggtcacatt caacatcaga cctttgccgt tggcacctaa
    17941 ataatcactg atcgcctgag cgccattttc actggtcgca gtgccaataa ctttcgcgcc
    18001 acgggctgcg agcgtttcag caattgcgcg gccaattccg cggcttgcac cggttaccag
    18061 tgcgattttt ccttcaaaat tcatgatttt cctcttttaa agctcgagcg ccgctgccat
    18121 cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg acaatgcgtt tcgtcaggcc
    18181 agtaagcacc ttgcccgggc cgacttcata gagatgttct acgccttgcg ctgccatgta
    18241 ctcaacagac ttcgtccact gaaccgggtt atacaactga cgtaccagtg cgtcacggat
    18301 ggcatcacca ttggtttcgc atttcacatc aacgttattc acaacagaaa ctgttggtgc
    18361 gttaaaggtg attttcgcta attctactgc cagtttgtcg gctgctggtt tcatcagcgc
    18421 acagtgagac ggtacgctca ctggtaacgg cagcgcgcgt tttgcgccag cagctttaca
    18481 ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca ataaccacct gtcccggaga
    18541 gttaaagttt accggagaaa cgacctgacc ttctgcagct tcttcacacg ctttcgcaat
    18601 agacgcatca tccagaccga tgattgccgc catagcgccc gtgccttccg gtacggcttc
    18661 ttgcatgaac ttaccgcgca tctcaaccag acgcaccgca tcagcgaaat caatcacgcc
    18721 agcgcaaacc agcgcggagt attcccccag gctgtgaccg gccatcattg ccggtgcttt
    18781 accgccctgc tgctgccata cgcgatacag cgcaacagat gcagtcaaca gcgctggctg
    18841 ggtttgccag gttttattca gttcttcagc tggcccctgc tgggtcagcg cccacaggtc
    18901 gtagtccagc gccgcagaag cttcagcaaa cgtttcttcg acaattggat agctcgccgc
    18961 catatcagcc agcattccaa cggtttgaga accctgtcca gggaacacaa atgcaaattg
    19021 cgtcatgttt taatccttat cctagaaacg aaccagcgcg gagccccaag tgaatccacc
    19081 gccaaaggct tcaagcagaa ccaactgccc cggcttaatg cgcccgtcgc gtacagcttc
    19141 atccagcgcg cacgggacag aggccgcaga ggtattaccg tggcgatcca gcgtcaccac
    19201 gacattgtcc atcgacatac cgagtttttt cgccgttgca ctgataatac gcaggttagc
    19261 ctgatgcgga accagccagt ccagttgaga acggtcaaga ttattcgccg ccagcgtctc
    19321 atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag acttcgttgc ccgccatcgt
    19381 cagatgaatt gaattctctg gattcacgcg gtcggcgttt ggcagcgtca gcaattcacc
    19441 ataactaccg tcggcatgca gatgggtgga aatgattccc ggctcttcag aggcagccag
    19501 caccgcagcg cccgcgccat cgccaaaaat aataatagtc ccacgatctg ttggatcgca
    19561 ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac ttcaccgccc cagatttcac
    19621 gtattgatcg gctacgctta atgcataggt gaaacctgcg caggctgctg caacgtcaaa
    19681 tgccgggcaa cctttaatgc ccaacatgct ttgaatctga caagctgcgc tcgggaaagc
    19741 gtgcgtagca gaagtcgttg ccacaacgat caggccaatc tggtctttct caatgcccgc
    19801 catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt gaaacggttt cgtttggcgc
    19861 ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc cactcgtcag aggtgtccac
    19921 cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc agatagctgc cagtaccaat
    19981 aatcttcgta tacatgtacg ctcagtcact tttcggttat ataccgtcac ttgcaaactg
    20041 cgagttcgct ggcagcgtcc tgctaccgca gagttccgct tttgccaccg tccagcagct
    20101 caaaaccagc tgggtataca gattccaggc gagcggcaat tcgctgagga acttgtcgct
    20161 gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc tcgctgattg gctgcaccat
    20221 gactttttat caccgtgccg cgcaatccta acagacaggc gccgttatac tggtcggggt
    20281 tgaggtgact gaatcgcctc gtcaggctct tttgtagcca acgctttaat aacagtagcc
    20341 accacgaccg ttttttccct tcaccctgag atttcagcag agaaaggaac atcctgacaa
    20401 caccttccat cgtctttaac gtgacatttc ctgtaaagcc gtcacaaacc agcacatctg
    20461 tcttgccagt taacaactca ttggcttcaa gatagccgat ataattgata gaagggattg
    20521 ttttaagcac cgctgaggca tcccgaatac tgtcgagacc ctttacttct tcttcaccaa
    20581 tattgagcaa cgccacgcga ggattgggaa tttccaccac ctcttcagcc agaactgagc
    20641 ccataatggc aaattgcacc aacattgtgc tgtcacaatc gacgttggcc cctaaatcga
    20701 ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt caccagcgcc ggacgctcaa
    20761 tcccctccag gggcttgagt aataattttg ccagccccat cagtgccccg gtattaccgg
    20821 cactgacaca ggcttgcgct cgaccttctt tcaccagctc cagggccata cgcattgaac
    20881 tcccacgact ggcgcggata gcttgcgaag gccgggcatc actggcgata actgactgcg
    20941 caggaataat ctgcagacgc gaacgttgtt caaagtcagc tttagcaagt aatggcgtga
    21001 tggcgtcggg attgccgact aaaagaagag tgagttgcga attagaattc agtgcctgca
    21061 atgctgcagg cactgtcacg gaagggccaa aatcccctcc catgacatct aacgccaggg
    21121 ttagacgttt caaggtatcg tcgccgcccg gtttggtctt tccccagttg cctggggaaa
    21181 atcctcacta agcttcatca cgcagacgcg tgattactta gcgatgacct tgcggccgcg
    21241 gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt tcaccagaag ttttgtctac
    21301 agacaggctg gtgactgcgg tcagcgcgtc atgggaacga cgcatgccac gtttggaacg
    21361 ggttggttta ttctgttgta cggccatgga ccttactcct caattacggt ttatccccga
    21421 tccaacgggg agcaccaatt acttacgctt taagctggct aatacggcaa atgggtttgg
    21481 cttttgcact tcttcaggca gttcaccaaa gaccatgtcc gcttcggaca cttcacagtg
    21541 ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg atttcatctt caaccattgc
    21601 aagcagatcg atttcaccga attcgttaac ctcaatcggt tcatacgctt ccggcagtgc
    21661 ttcagcctgt tcgtctgaac gcacaggact aaaacaatac gttgtgtaga cctgatgagt
    21721 aaacggcttc ccgcaacgct gacactcgag cgttaccgtc accttcgcat cgccgtttaa
    21781 cactgcgaga cgttggttat cgatagcgaa cgacatggag cattccacat cactgtccac
    21841 actgactacg gattcggcga cgcgctcaac ctgatcaggg gtatagatac cctggtaatc
    21901 aaggcgtttt tgagccgtac gaaccggatc gagagtcagg ggtaatttta ccttttgcat
    21961 agggcgcgca tattaacttt gtaacgtcat agagtcaaag aaaaaggcag cctgtggttg
    22021 ccttttgcca ataattcgca cacattgcgg gttactgctt tattttcgtt cagtgctgat
    22081 aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa tggctatcat caatacgcta
    22141 tatctgatgg aaaaaaatat gcctaaactt attttagcct ccacctcgcc atggcgtcgc
    22201 gctcttctgg aaaaactgca aatctctttt gaatgtgcag caccagaggt cgacgaaacc
    22261 ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac tggcacaaga aaaagcgcaa
    22321 tctctggcgt cacgttatcc ggatcattta attattggtt cagaccaggt atgtgttctt
    22381 gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg cccgtctgca attacgcaaa
    22441 gccagcggca atatcgtcac cttctatact ggactggcgt tgtttaattc ggcgaatggg
    22501 catctacaaa cagaagtgga gccttttgac gtccatttcc gtcacctaag cgaggcggag
    22561 attgataatt acgtgcgtaa ggagcatccc ctgcactgcg cgggtagctt taagagtgaa
    22621 ggatttggca ttacgctgtt tgagcgttta gagggacgtg accctaacac gctggttggt
    22681 ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag ggaaaaaccc gctgatggga
    22741 tagttgtttg cgccggatgc ggttacgtcg catccggcaa tcggtgcagg cggcctgatg
    22801 cggcgtaaac gccttatcag gccaaccgcc cattatcagg cttatatgct tgtattagcg
    22861 cgcgttacgc agcttttgca aacaacgctt caaaccttca tccatcggcg cttcgatacg
    22921 catcacctca ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca ggaacagacg
    22981 atttaatccc gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac cgtagcgatc
    23041 gtcaaaggca atcggatgac ccgcatactg tgtatgcaca cggatctgat gagtacgtcc
    23101 ggttaccggg ctacaacgca ccagggtggc aaatgcatag cgttcttcca ctttaaagcg
    23161 tgtttccgac ggtttgcctt cctgactgac acgcacgata cgttcgccgc tttgcagaat
    23221 atttttcaat aacggcgctt gaacgctctt cacatgcgac tgccactgac cgcgcaccag
    23281 cgccaggtaa tctttttgca ttcctttttc acgtaattgc tcatgcagag aacgcaacgc
    23341 cgagcgtttt ttcgctacca gcaacacacc tgaagtgtcc cggtcgagac gatgaaccag
    23401 ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc cgaagcttaa
    23461 accactgccg ccatgtaccg ccgtaccgga aggtttattc agcaccagga tatgatcatc
    23521 ttcatataag atgacgtccg ccagcgccgc caccttttgc agatgtggcg aaaccgcctc
    23581 ttcttcccgc tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg cttcgagttt
    23641 atattcaggc ttaatacgtt ttttgttcac ccgcacttca cctttacgca aaatacggta
    23701 aatcatactt tttggtacgc ctttcaattg ggtacgcaaa aagttatcga tacgttgccc
    23761 cgcttcgtca gcggtgatag caacaatttt tacggatgga gtctctgttt tcatggtggg
    23821 cgattctaat tagccaacag gattcgcgcc actcattttt ctatgcttat atttagtttt
    23881 gcaccttatt acttcactac gtgatcactt tattgatggt tattaaacca atcaccagca
    23941 agaagtgaaa aaactgtgag taagcgggtg ataaatggta aaagtcatct tgctataaca
    24001 aggcttgcag tggaataatg aggccgtttc cgtgtccatc cttgttaaaa caagaaattt
    24061 tacggaataa cccattttgc ccgaccgatc atccacgcag caatggcgta agacgtattg
    24121 atctttcagg cagttagcgg gctgcgggtt gcagtcctta ccggtagatg gaaatatttc
    24181 tggagagtaa tacccagtct gtttctttga taattgcgct gtttttccgc atgaaaaacg
    24241 ggcaaccgac actctgcgcc tctttgagct gacgataacc gtgaggttgg cgacgcgact
    24301 agacacgagg ccatcggttc acacccggaa aggcgttact ttgcccgcag cttagtcgtc
    24361 aatgtaagaa taatgagtaa gttacgatga aaagaatgtt aatcaacgca actcagcagg
    24421 aagagttgcg cgttgccctt gtagatgggc agcgtctgta tgacctggat atcgaaagtc
    24481 cagggcacga gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc attgaaccga
    24541 gtctggaagc tgcttttgtt gattacggcg ctgaacgtca cggtttcctc ccactaaaag
    24601 aaattgcccg cgaatatttc cctgctaact acagtgctca tggtcgtccc aacattaaag
    24661 atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga taaagaagag cgcggcaaca
    24721 aaggcgcggc attaaccacc tttatcagtc tggcgggtag ctatctggtt ctgatgccaa
    24781 ataacccgcg cgcaggtggc atttctcgcc gtatcgaagg cgatgaccgt accgaattaa
    24841 aagaagcact ggcaagcctt gaactgccgg aaggcatggg gcttatcgtg cgcaccgctg
    24901 gcgtaggcaa atctgctgag gcgctgcaat gggatttaag cttccgcctg aaacactggg
    24961 aagccatcaa aaaagccgct gaaagccgcc cggctccgtt cctgattcat caggagagca
    25021 acgtaatcgt tcgcgccttc cgcgattatt tacgtcagga tatcggcgaa atccttatcg
    25081 ataacccgaa agtcctcgaa ctggcacgtc agcatatcgc tgcattaggt cgcccggatt
    25141 tcagcagcaa aatcaaactg tacaccggcg agatcccgct gttcagccac taccagatcg
    25201 agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct gccatccggt ggttccattg
    25261 ttatcgacag caccgaagcg ttaacagcca tcgacatcaa ctccgcacgc gcgacccgcg
    25321 gcggcgatat cgaagaaact gcgtttaaca ccaacctcga agccgccgat gagattgctc
    25381 gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat cgacttcatc gacatgacgc
    25441 cagtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt caggaccgtg
    25501 cgcgtattca aatcagccat atttctcgct ttggcctgct ggaaatgtcc cgccagcgcc
    25561 tgagcccatc actgggtgaa tccagccatc acgtctgccc gcgctgctcc ggtaccggta
    25621 ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg tttgatcgaa gaagaagcgc
    25681 tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt gccaatcgct tcttatctgc
    25741 tgaatgaaaa acgttctgcg gtgaatgcga ttgaaacgcg tcaggacggt gttcgctgcg
    25801 tgattgtgcc aaacgatcag atggaaaccc cgcactacca cgtgttgcgc gtgcgcaaag
    25861 gggaagaaac gccgacctta agctacatgc tgccgaagct gcatgaagaa gcgatggcgc
    25921 tgccgtctga agaagagttc gctgaacgta agcgtcctga acaacctgca ctggcaacct
    25981 ttgccatgcc ggatgtgccg ccagcgccaa ccccagctga acctgccgcg actgtcgtag
    26041 ccccagcacc gaaagctgca acggcaacac cagcagctcc tgcacaacct gggctgttaa
    26101 gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg tgaagaagcc aaaccgaccg
    26161 agcaaccaac accgaaagca gaagcgaaac cggaacgtca acaggatcgt cgcaaacctc
    26221 gtcagagcaa ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa cgtactgaag
    26281 gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag caaactgccg
    26341 agacgcgtga gagccgtcag caggctgagg taacggaaaa agcacgtacc accgacgagc
    26401 agcaagcgcc gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt caggcgcaac
    26461 aagaagcgaa ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa caggaagaac
    26521 gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa gtgcgttacg
    26581 agcaaagcgt agccgaagaa gcggtagtcg caccggtggt tgaagaaact gccgctgccg
    26641 aaccaattgt tcaggaagct ccagctccac gcacagaact ggtgaaagtc ccgctgccag
    26701 tcgtagcgca aactgcacca gaacagcaag aagagaacaa tgctgataac cgtgacaacg
    26761 gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt ggtcagcgtc
    26821 gtcgtcgcta tcgtgacgag cgttatccaa cccagtcgcc aatgccgttg accgtagcgt
    26881 gcgcgtctcc ggaactggcc tctggcaaag tctggatccg ctatccaatt gtacgtccgc
    26941 aagatgtaca agttgaagag cagcgcgaac aggaagaagt acaagtgcag ccgatggtga
    27001 ctgaggtccc tgtcgccgcc gctgtcgaac ctgttgttag cgcgccagtt gttgaagaaa
    27061 tggctgaagt cgtagaagcc cccgtaccgg ttgccgaacc gcaaccggaa gtggttgaaa
    27121 cgacgcatcc tgaagtgatt gccgccgcgg taactgaaca gccgcaggtg attaccgagt
    27181 ctgatgttgc cgtagcccag gaagtcgcag aacacgcaga accggtggtt gaaccgcagg
    27241 aagagacggc agatattgaa gaagttgccg aaactgctga ggttgtggtt gctgaacctg
    27301 aagttgttgc tcaacctgcc gcgccagtcg tcgctgaagt cgcagcagaa gttgaaacgg
    27361 tagccgcggt tgaaccagag atcaccgttg agcataacca cgctaccgcg ccaatgacgc
    27421 gcgctccggc accggaatat gttccggagg caccgcgtca cagtgactgg cagcgcccta
    27481 cttttgcctt cgaaggtaaa ggtgccgcag gtggtcatac ggcaacgcat catgcctctg
    27541 ccgctcctgc gcgtccgcaa cctgttgagt aataattagc tcaaagtaat caagccctgg
    27601 taactgccag ggctttttta tttcatgttt gaatcaacat attaaaattc atcaaatggc
    27661 atccttgcta accaacaatg caaaataggc aaaaaaaatg cccgccccgg cgatcccaga
    27721 gcgggcagac aaaacatacc cagtttcatg atatgtttta aagcgagctt atttgttgag
    27781 ctggaagagc gacaatccct gcatatcggt aaatgcttta tacgatgcct gcaatgccgt
    27841 ttgctgcatg atgtaagatg aaatagttgc attccagtcc acatcaacca gatcgctcat
    27901 ctgctgcgtt tgccctaaag cgcgatcgct acctaaggaa tccagcgact ccagtttgtt
    27961 cagctgcgtg cctaattccg cgcgaacggt aaggacatta ttcagcgagt ttttcagtcc
    28021 acggttggtt ttatccaatg ccgcagcggc gatttcttta tccgcttcgc tatccgcgac
    28081 cggcgttttc agggctgcga tggcgctatc cagcatggca aaaagattgg tttcagaagc
    28141 gctaccgtct ggttccgcta ccgcgttact ggtaatactg tcgaaaattt tgtcgcccgt
    28201 atgtcctatc accatcgaac gcgaagcatc gacctgttgc ttaatacttt ctgttccgcc
    28261 gacataatca ccgttagcct cactaaacgg cgcagtctct gttttataac cggcaaaaat
    28321 gtagcgccca ttaccgtcag tggtgttcgc cagattcagc aactggtcac gaagcccctg
    28381 aatatccgta gccagcgagg cccggtcatc gtcactcaag gtgccattgc tggcgtagac
    28441 aattttttcc tgagcattct ggatagcagt ggtgacctgg ctaagtacac tctcttccag
    28501 tgacactttt tgagtggcga aagtacgtgc cagcgtgtac tggctgtttt gcgcctgtgc
    28561 ctgggagaga acaacagctt gtgatgcagc aatgggatcg tctgaagggt taacgactcg
    28621 cttacccgtc gatatctact cgccgtactt catccattct gcctgagaat tggtgatgcc
    28681 gcgcatgttt tgctggtaca tcatctgggt actgaaacgc attttatctc cccttagcga
    28741 atgttaatca gagcatcaaa aatcgtgttt gccgtctgca gaacctgcgc attcgccagg
    28801 taatactgct gaaaacgttg cagatttccg tactcctcat cgagattgac accggaaatc
    28861 gactgctgct gattggaaag ctgcgtcacc acattacctt gcgtggtgct actggttttc
    28921 aacatcgcgg ttttattacc gatatcactc actaacgagg cataagcgtc gttaaaggat
    28981 ttcgcaccgc ccaccgtttt actgttgctt tgcagatcca gcagggcctg accgttgcgg
    29041 ttgtcgctat cacccgcatc ttcttcgctc gccatcgcga ttttcgcttc gtcggtgatt
    29101 aatacatcca tgttgacgat ggcgtcactt actggtttca gcgtgaagct gtcgttaacg
    29161 gcaggcgttc ctgtaaacgt caactccaga ccatcaaatg ccactttacc gttggcatct
    29221 ggtgtcgccg taaaagtggt attgctggca aggcgggtga cctgccactg attattatcg
    29281 aacgagattt tgtaatctgt cgccagtacc acggaggcat cagttaccgt ggcaccgatc
    29341 gcaacgtcac ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat agtaaagaaa
    29401 tcttcaccag catcgccgtt agcatcaaat ccggctttgt gttggctgtt gaaagcctcg
    29461 gcaaatgcca gcgccagttg tccaagcgta ttacgcgtct ggtccagatc ctgagaacgg
    29521 aatgtcagaa tgccgcccag cgacccggta ttcagtaatt tctccgggat ctcaatattg
    29581 cctgccgtcc catcaacgta agcgacagtc gtacgagaag ggtcggcgct ggaaggaact
    29641 gccgccagtt gccgcgccgt acttccctga accagtgagt aaccattggc atcgtgatgt
    29701 tataagtacc gccatcctga acgctgactt ctacgccaac aatctggttt aattcgctca
    29761 ccagttgatc gcgttgatcc agcagattgt taggtgacgc ccctgccccc acgcctgtca
    29821 ggcgcgagat ttgatcgttc aggctggcaa tttgtttagc gtaattgttg atctgatcaa
    29881 cgctggcacc tatcgcgata ttgacctgtt tgtcctggtc gcgcagatat tgatcggtgg
    29941 ttttaaactg attcaccaac ccttctgatt tcccaatcag cgcctggcgc gccgccgggt
    30001 cttccgcgtt actcaccagc gtttgcaggc tggtgaagaa atcctgcatc tgtgttgcca
    30061 gcgaagaggt actggtggag agcatattgt cgattttcga catctgctca tagcgggcgg
    30121 tcaggccgct actttgcgtc tgcgcagcac gtaactggtt ggtaataaac gtatcatact
    30181 cacgctgcac accagaaacg tagacgccat tgccaaccca gccgccagcg cccaacgtgc
    30241 tattggcctg cgccataata gtggtttggc gggtatatcc ggcaacgtta tagctggaga
    30301 tattattact tgccgtattt aacgccgcct gggccgcgtt cagtccgctc atggcgttat
    30361 tgatcaagct ggacatggag gttccttata agccttcaat tacagagtat tatcggcagc
    30421 gacccgccgg acttgagtta ttcagaacag attatcaatg ttcatgctgt aggttttgct
    30481 caccttgtcg cttatcgatt tcatctgctg gatcatgttg gtgagtttac gggcatagtg
    30541 aggatcggta gcatagcccg cgtcctgtag gacctgcgcc ccctgttccg cactcgcggc
    30601 ggtcgtcacg gcggcgtagc gcgggttacg tgttaacagc ccaacgtaat ccgacaatgc
    30661 ttccagatac gagctgtaga cccgaaactt cgcttttact ttcttcgctt cgccgttttc
    30721 atattcagtc gtggtgattt cagtgacttg ccctttccag ttgccagagg ctttgacacc
    30781 aaacaggtta tagcttggct cgccgttttc gcggcggatt tgccgttgcc cccaaccaga
    30841 ttcaagcgcc gcctgagcga ggatcaaatg atgtggcaca ccgctttgct ggcttgccag
    30901 ttgggcgggc agcgagagtt gcgcgaggaa tgctctactg tcacccggca gcgaatcatc
    30961 gtagttacgt ggtacggcct tttgcaccag ctgcgaaagc gtctggtttt gataacgcac
    31021 cacggtttcg agcgggaatt tcatcggtgc tgctggcgtg gactcctctg gcaatggttg
    31081 ttctggcgtc atctgtttaa ccatcatctc tgcaagcccc agacctttgc ccgccgtcat
    31141 ctgttgggca atttgctggt catacatact ggtatacaga cgagtgtgct cgctgctgaa
    31201 caggccatct tttggtaaag cgtcgcgcat gcttttcaac atcatctgca cgaacatccc
    31261 ttccacctga cgggccaccg gacggatatt tgccgccgga tcttcgctcg atttcgcctt
    31321 tagttcgttg agtgattgcg catcccaggc cgcacttgcc agtagtttgc tgtcgctgat
    31381 catcagatga tttccagttt tgcccgcaga catcccgcac tttgcattga ttgcaaaata
    31441 gacatcagat ccatcggcgt agcgcccagc gcattgagcg cacgcaccac gttattgagg
    31501 ctggcgctgg aacgtacgct ttgcagcgaa ccgccgctct ggcgtaaatc gatctgcgtt
    31561 tgtggagtaa caacagtctg tccaccacca aacggtgtat ctggctggct gacattggcc
    31621 tgacgattaa ctgttactga gagatttccc tgcgctaccg cgcagctgtc gagggtcact
    31681 tcgcgattca tcaccaccga accggtgcgc gagttaatca ctactttagc gtcctgcggg
    31741 gtgacattaa cctgcatatt ctggatatcg gcaaggaaac ggacctggga actgttgcca
    31801 cttggtacgc gcacctgaat agtccgcgca tctaacgcgg tggcgctgcc atatccacgc
    31861 acgcggttga tggtgtcagc gatttgctgc gccatgctga aatcttcgtc gttaagttgc
    31921 aaattaaggg tattcccgac gccaaactgg ctgggcaatt cacgttcaat aaccgcacca
    31981 ttggtgatcc gtccaccgtt cagttggtta acctgaacac tgctaccgcc cgcggaggct
    32041 cctgcgccgc caaccagaat attgccctgc gccagcgcat acacctgact gtcaacgccc
    32101 ttaagcggtg tcatcaacaa cgtacctcca cgcaagcttt tggcattacc catggaagaa
    32161 accaccacat cgatggtttg cccctgacgt ccaaacggag gaagtgacgc tgtcaccatt
    32221 accgcagcga cgttttttag ctgcatattg gtgcccgtcg gaacggtaat tcccagctgt
    32281 gagagcatgt tattaagcgt ttgtgtggta aacggcgtct gggttgtctg gtcaccggtg
    32341 ccatccagcc ccactaccag gccatagcca atcagtgagt tttgccttac cccctgaaca
    32401 ctggtgagat cgcgaatacg ctcagcctga gccgccgtcg taaccagtag aagaattaat
    32461 gcagagagaa atttaatcac cacagcctca cttacattgg cgacaggtta aggaagaaac
    32521 gctgcaacca gcccatattt tgcgcttcgt taatgtagcc attgcctacg tattcaatac
    32581 gcgcatccgc cacctgagta gacggtacgg tattgctgcc gctgatagtg cgtggattaa
    32641 ccacgcccga gaagcgaata aattcggtac cctgattaat ggcaatctgt ttttcaccca
    32701 ccacatgcag gttgccgttg accagtacct ggtcaaccgt caccgtcaac gtgccgctaa
    32761 aggtattgct ggcattggcc ccgccctttc cgttgaacgt gttaccaccg gaggcttcga
    32821 catcggcacg agcgttacca aacagcccct gcaaatagcg cggcacagta tcaaagccaa
    32881 aattagtttt accgtcacgg ctgacattcg cagaggagct tttgctggcg ctgacgttct
    32941 cctgcaacac gatggtcagc gtatcgccaa tattgcgtgg tcgacgatct tcaaacagcg
    33001 gttgatagcc atagttaatc ggctgagcag actggaaaat agaaccgttg gcgacgggcg
    33061 tcggaccggg aaccggttgt gcactggtcg ccccctgcac cagcggcgtg gagggtatcc
    33121 aggcgcagcc ggttagtgaa agcaccaaca agctggaaat ggcataagta tgcgcagcgt
    33181 ttttttgcat ggctatcatc ttcaaaaatc agtaaaccgg tgaacctgcc accggttatg
    33241 ccttagagtt gcgtcagttt ttgcagcatc tgatcggtgg tggacaccgc tttactgttg
    33301 atttcgtaag cgcgttgcac ctgaatcata ttgaccagtt cttccgccac gttgacgtta
    33361 gacgtttcaa catacccttg atacagcagt cccgcgccgt tcaggcccgg cgtgctttcg
    33421 ttcggtgcac cggaggattg cgtttcggtg tagaggtttt cgccaatgct ctccagtccg
    33481 gtatcattca taaaggtggt gagattgagc tgcccaacct gaaccggagc tgcctggcct
    33541 tgttgggtta cgctgaccac gccatcacga ccgatggtga tacttaacgc attcgccgga
    33601 atggtgatcg ctggctgcac ctgaaaacca ccagccgtca ccagctgccc gttctgatcc
    33661 acctggaaag agccgtcacg ggtataggct gatgagccat ctggcaacat tacctggaaa
    33721 aagccctgca ctttaatcgc gacatctttg ctgttattgg tctgcgacag attgccctgg
    33781 ctgtgtaagc gttcagtggc gaccgggcgt acccccgtgc cgatttgtaa tccggagggt
    33841 aaggtggttt gttcggaaga ctgtgccccc ggctggcgaa tggtttgata aagcagatct
    33901 tcaaacaccg cgcgctgacg cttaaaaccg ttagtactga cgtttgccag gttgttggca
    33961 atgacgtcca tattggtttg ctgggcgtca aggcccgttt tggcgatcca taatgaactg
    34021 atcatataat gtcctgtagt ggattgctga caagcagagc agaagcacgc gacagtgtga
    34081 atttcccctc accctaaccc tctccccaaa ggggcgaggg aatagtccgc gcgcttttat
    34141 tgcaatgtca tcaatcgttc aattaactca tcgacagcag ttggttggca cggcctgcgt
    34201 tatcatcgac gctgctgatc accttcatct gcatttcaaa acgccgcgcg ctggcaatca
    34261 tgtcgctcat tgccgcaacg gcattgacgt tactgccttc cagaaccccc gacatcacac
    34321 gcaaggttgg atctgcctgc agtaccggcc ctcgcgtggc ctgggtttct gcgcttaaac
    34381 gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt ggctttcatc agtttcagac
    34441 gccctactgg cgcaactgta tttgccggat cgcccggatt gagcgccgag attgtgccat
    34501 cggcagcaat cgtgatttcc gccccttccg gcacagcaat tggccccgcc tcgcctatca
    34561 ccggatgccc ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg ccattacgcg
    34621 tatacccttc gctgccgtca gcggtctgca cggccagcca tcctgctgca acgcaacgtc
    34681 cagcgggcac gaggtgtaat ccatcttgcc gggcgtcatg tctgcacccg gtgttgacgc
    34741 cgttaccagc gtgcgcgtgg gcagtgaaag cccttccacc ggcactgcgc gcaaggcgtt
    34801 taactgcgcg cgaaaaccgg gcgtagaggc attggccaga ttactggcgg ttaccgcctg
    34861 ttgattcagc gtctggctgg ctgctcccat cgcagtataa attgcgtgat ccattgagct
    34921 atcccgtcag cgattagcgt aagttaacca gcgtgttgag gatctggtcc tgggttttga
    34981 tggtctgggc gttagactga tagttacgct gggcaacgat catattgacc agttctttac
    35041 tgagatcgac gttggacgct tccagcgcac cgttggtcag ggtgccaaag tttcccgtcc
    35101 cggctgtccc caacagcgcc acgccagaag attgcgtcgc agaccagacg ttgtcgcctt
    35161 cggatgccag accttcgttg ttggcaaagt tcgccagtac aatccgcccc agcagttggg
    35221 tttgttcgtt ggaatagttg ccgacaaccg taccgtcatc attgatttga taactcacca
    35281 gatcgcccgg tttgtagcca ttctgggtgg ttgccacaat gttgttagcg ccggtatttt
    35341 gctgcatgga gttgaggaag ctcagactaa acgtggcggg ttctgcaccg ttaattgcgc
    35401 cggttgcgat attattcgcc atcgcaccat ccactaatgt gccattagca ttaaattcca
    35461 gtgttgtcgc tgtcttcgca atgctgtttg gatcactgct atcctgggtg tagacctgcc
    35521 agttattatc cccggtcttc acaaaataga cgctcatgtc atgagcatta ccctgactgt
    35581 cgaaaacagt caccgaacct tttttgttat agctatccgc attgctggcg ctgaatggcg
    35641 taacagtagg aagcggatca ctggaattca ggttgatctg catcgacgcc gtggtggtag
    35701 ttttcgctgc catcagggta ttcgggatcg aaatattggt cggattcgcc ccttgctgaa
    35761 tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg taaaccttgc atattcacca
    35821 ggttacggtt ttcatccagc ttaaattgtc cgttacggct gtagaacacc gaaccgttgc
    35881 tgtctaccag acggaaaaaa ccgttctggc tgatagcaac gtccagacct cgcccggtgt
    35941 tggtggtcgt gccatcggta aagtcctgag tgataccggc aacttttacc cccagtccca
    36001 ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc tgatttaaag ccgtaggtgg
    36061 cggagttggc gatattgttg ccaataacat cgaggttggt ggcggcagcg tttaatccgc
    36121 taaccgcttg agaaaaggcc atgactgact cctgaaatgt gaaggcttaa attatctgcc
    36181 gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc cagcgtatta ccgctgttgc
    36241 cgcggatcac accctgcacc agagcaaact gcagcggctg ggcaaccagt tgtgtaccac
    36301 cgttactggc gctaatcgct acattgtaag aaccgttcgg cgcagtgctg ccatcagtca
    36361 acgtaccgtc ccaggtgaaa ctgggaactc cggcggtcag ttcaccaata tcaatggtgc
    36421 gcacaaccgc gccattttta tcggtgatgg tggccgtaac tttgtctgcc gcctgttgca
    36481 gctcaacacc aaacggcgtg gtcgtggtca cagccccttc ttcactgccg gttcccgcaa
    36541 gaacagttgt gccggggatc atcacgccgt gaccgatcag gttactggcc tgtaacgact
    36601 ggctgttatc aatctgtccg gaaatagatc cgagcgtggt attgagtttt tcaatcccgc
    36661 tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt gttttccatt ggattggtcg
    36721 ggtcctggtt tttcagctgc gccaccagca aagtcagaaa actgctttgt aaatctgcgg
    36781 cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac gccggtattt gtcggatcgg
    36841 tggtggttac cgcaatggac atagctttct cctttattga ccgagcgtaa gggttttcag
    36901 caccatgctt ttcaccgtgt tgagcacttc aacattggcc tgatagctgc gtggcgccga
    36961 catggtgtta accatctccc cgacgacatc aacgttcggc atttttacgt agccctttgc
    37021 atctgccagc ggattacctg gttcataaac cagtttgtcc ggggcctgac tttctataac
    37081 atcggcaacc tttacgccgc ctgtcgcagc acctggtgca gcgttaacct ggaataccac
    37141 ctgttttgcc cgatatggct gtccatcagg accggtcacg ctatcagcat ttgccagatt
    37201 actggccgcc acgttcaggc gctgggactg ggcagttaac gccgacccgg cgatatcaaa
    37261 aatattcagc agtgccatcc gttaatttcc gctctgtaaa acgttcatca tgcctttgat
    37321 ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg ctgttatcgg caaactgggt
    37381 gcgttcgcga tccatatcga cggtattacc gtcaagcgaa ggctggtccg gaatacggta
    37441 ttgcagttct gcggtaggag gcgtcagcgc ctgcgccggg atgtgttgcg ttgaggtcat
    37501 cgtcagtgca accacactgg ttgcatcccg tccacgttgc atgacttttt taagttcact
    37561 ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg gcattggcga tgtttgctgc
    37621 cagcacttcc tgacgctggg cgcgcagatt gagcgcctct tgttgaaaac gtaaggcggc
    37681 gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa ttctgccatc agcttaaatg
    37741 cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg gtcgctattt atgccgttga
    37801 tggtcattgc ggacaggtac aattcacgtt gtagaaatgg ctgggggcga aaatgctgac
    37861 aataaaacgt agcgtggcga tcatcgcgat actgttcagt ccgttaagta cggcgagcaa
    37921 tctcacatcg caattgcaca acttttttag cgcccaactc gtgggggtaa gtgatgaggt
    37981 tcgtgtttct attcgtacag cgcccaatct actaccgcca tgcgagcagc cattgctttc
    38041 gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct gcggtaacga
    38101 caaacgatat ttacaggtta atgtgcaggc cacaggaaat tatgtggttg ccgcgatgcc
    38161 tattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctgaaacgtg gacggctgga
    38221 taccctgcca ccgcgtacgg tgctggatat caatcaactt gttgatgccg ttagcctgcg
    38281 cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat ggcgggtaaa
    38341 agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg tttagcgcca acgcagaagg
    38401 tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg gtgcgcatgg tatcgggaca
    38461 ggtagtcagc ggcgttgttg atgcagatgg gaatattctt ataaacctgt aagctgttaa
    38521 agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc tcaatgagga
    38581 ataaaccatg agtattgatc gcacttcgcc tctgaagcct gtaagcaccg ttcaaccgcg
    38641 cgaaaccact gacgcgccgg taacgaacag ccgggcggca aaaacaaccg cctccaccag
    38701 caccagtgtg acgttaagcg acgcgcaagc aaaactgatg caacccggca gcagtgatat
    38761 caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac taaaaatgga
    38821 caccggcaaa attgccgatg cgctgatcaa cgaagcgcag caagacttgc agagtaactg
    38881 atcgtatgac acgtcttgca gagatcctcg accagatgtc cgctgtgctt aacgatctca
    38941 aaacggtaat ggatcaagag cagcaacatc tctctatgag gcagatcaac ggcagccagt
    39001 tgcaatggat tacagaacaa aaaagctcac tgctggcgac gctggattac ctcgaacagt
    39061 tacgcaagaa agaacccaat acagcaaata gcgttgatat tagtcaacgc tggcaggaaa
    39121 ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg ttactggaag
    39181 gacagattga gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag gaaccgacgc
    39241 tatatggggc gaacggtcag acctcaacac cccatcgcgg cagtaaaaag atttcgatct
    39301 gaatattatc tccggcctgc actgcaggcc ggaatgcatt gttacaccgt ccggcgggca
    39361 aattctttaa ctttgaagcc cagtaccgcc agtgcagcga agtacgcggc aatccccgcc
    39421 agcacgaccg ccattaaacg cagtaagcgc cagggcatgg tacccaatga ccactccggc
    39481 atgatatgta acatacctaa aagcacgcca gacatcacca gtaccgccac caccagacgc
    39541 aacagaaacg ccatccagcc gggttgcgga gtaaagattt tctgcttacg caactgccag
    39601 taaagcagcg aagcattcag acacgccgcc agaccaatag aaagtgacag cccggcatgt
    39661 ttcaacggac caataaacgc caggttcatc aattgcgtta aaatcagcgt aacgatggca
    39721 attttcactg gcgttttaat gtcctggcgg gaataaaagc caggagccaa cactttcact
    39781 acaatcaggc caatcaaacc caccgagtag gcaattaacg cccgctgggt catcagcgca
    39841 tcaaacgcgg taaatttacc gtactggaac agcgaaacgg ttaacggacc tgaaagaatg
    39901 cccaacgcaa ccgcactcgg cagtgccaac aggaaacaaa gacgcaaccc ccagtccatc
    39961 aaacggttgt attcatcatg attgccactg gcaaaacttt tcgacagcga cggcagcaaa
    40021 atggtgccaa gcgccacgcc cagcacaccg gagggaaact ccattaagcg gtcggcgtaa
    40081 tacatccaag acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt gatgattaag
    40141 gagatctggc tcacagagac gccaaggatc gccggcccca tctgtttcac cacgcgcatt
    40201 gctccggcat cgtggaagtt aatgcgcggc aggaccagca tgccgatctt cttcaggtgc
    40261 ggtagctgat acaccagctg caggatgccg ccgaccgtaa cagcccacgc cagcgccagc
    40321 accggtgggt taaagtacgg tgcggcaaac agcgcgaaac caatcatgct gatgttaagc
    40381 agtgttggcg caaacgccgg aatcgagaag cggttccacg tattcagaat cgctcccacc
    40441 agcgacgcca gggagatcag caagatatag ggaaaggtaa tctttagtag ctggctggtc
    40501 agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat cacccacggt
    40561 gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa gtgtcagcag gccagaaaca
    40621 taagagacaa agacccgcgt ggcgtcttca ccctgcttac ttttatattc cgccagaatc
    40681 ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa gttaggaagt
    40741 ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc caaagattct ggcgacaatt
    40801 gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca tcgagctgac ggcggccagc
    40861 gattttaata aattcatcgg tgttctaatc cggacccacg ggcaaaacgc ctgcaaggca
    40921 ggcgctgaaa gaggcgaata gtctacctgg attatggtga attgctaccg ccagatgtta
    40981 cagggtttat tcactcatcg catcgcgcca gatcttgtca acgatacgtt gcgccagcac
    41041 tgcctgttcg ccggcggttt gcggaactgt ctgattttgc acgcattcaa tgaagtgccg
    41101 cgcacagccg acaaatccac gttgctcaag agtgctctgc caaccaggaa tcggtttatt
    41161 cactacaccc tgcccgcgct cctcacgcca ttcgcgcata tccgtaatgt cgatgagcgc
    41221 accgtcagtc acggcctgca cggtttcacg ctgacttccg gcacggcgat gcatacaagt
    41281 ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca aacagcattt cgccagcgtc
    41341 gttagtcagt agcgtaccgc catccagaga ggctttgccg cccgacaacc acagcgcggt
    41401 atccaccaca tgcagataat catccagcaa cgtgaaataa agatcgtgtg gcccgacgct
    41461 attgctacga tgtttatcca ttcttagcga ggctgcggtg gcgagttgcg tttttaactc
    41521 accgtagagt ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt ttttccgcgc
    41581 cgccagttcc accagccttt cagcatcgcg cagattttct gccagcggtt tatcgacaca
    41641 gacatgtacc cccgcattga gtaacgtact gaccacgtca aagtggctgg cggtgctgga
    41701 gtgcacaaaa accgcatcac aactggcggc aaggctggat aacgaatcgg cataaggaat
    41761 gcgccagctg tcacaaattg gcagggcttt ggcgcgcgta ggcgaccagg ctccttgtaa
    41821 cgtccagtca gacgctgccg ccagcaccgg taaccacgct ttttgcgcaa tgccgcctaa
    41881 tcccactacg ccgatacgta attttttcac tttaatctcc cagatgggcc agcaacgaat
    41941 caagacgttg tttcagttct gccacttcga tttccagggc ttcgacgcgg gtctgtaaat
    42001 caccgtcaac cacgttcgac atagccgtta ccgccggctg atcttcaacc tcaccactga
    42061 aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc accacaaaag
    42121 gaccatcttc gcgattcgcc agttgttcca gcgtcgactc cacttccgcc atatcgctga
    42181 attcatacat tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca ccacgcaata
    42241 acaacgtggt gattaacgcc acttctgcgg cgctcagttt cagatcgcca aattctgaat
    42301 tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa accgctcact gtgcgtagat
    42361 aatgacgttt gaccagatta tccagctgtt cctgcacttc ggattcactc agattcatga
    42421 ccggttcacg gttcgttttc tgattacagg ccgtgactac accattgact gagagcgggt
    42481 attgctccgg cgtggtcacc tgcttttcca gcaagcaacc aattacgcga gcttccaggg
    42541 cagtaagttg atatttcatg agattctcct tagcggccgg gcgtccagtc tggggtagtt
    42601 aatgccgtca gtacgtgatc gcgccattgt ccatcaatca acagatagtc tttcgcatag
    42661 ccttcttttt caaaacccag tcgcgccagt aaatcaccgc tgcgtttatt gtgcggcata
    42721 taattagcca taatgcgatg aatatgttgg gtgcgctgca tataacgaat ggctgcggtc
    42781 agggcttcaa acatgagtcc tttgccctgc catttttgcc caatcgaata accgagatag
    42841 caggcatgaa aagagccgcg aacaacattg gaaaaattgg caacgccaat aatctctttt
    42901 tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt gtttatgaaa ttcgttaatc
    42961 atccctagcc tggcctgcca gcctgatgga taacagtggc tttcgtcgcg cactggctcc
    43021 cagggcttga ggaaatggcg attctctgcg taataatccg ccagacgcca ggcatcacga
    43081 tcatgcacca gacgcacgac cagtcggtct gtggttaagc gcacttttgg cacgttactg
    43141 cgatagccaa acattctata cctactcctt cccgtaacgt ctttaatacg ctgtaagtat
    43201 tactatacct gcgcaaccag accctgtgaa aagtccaaaa tgccattttc tgcattattc
    43261 acatatttga taaaaactct ctatcaaagc gacgttttga taaaaaaata ttgtccccga
    43321 agtgatggga aaagtcttag cggcaatgca gaatagaaag tgctcatctt ttatttcccg
    43381 ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg gtaaatattt cctgctcatc
    43441 gataatatgc tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc tatccgcttc
    43501 gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg ctctcggtct acgccagttt
    43561 attcagcaag gtctgggtat tttcggcggc gcaattgccg accgctttgg tgccaaaccg
    43621 atgattgtta ccggtatgct gatgcgcgcc gccggattcg ccacaatggg tatcgcccac
    43681 gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac tcggcggcac gttgtttgat
    43741 ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac aacaacgtgg tcgttttttc
    43801 tcgctgttga tgatgcagga cagtgccggt gcggtcattg gcgcattgtt ggggagctgg
    43861 ctgttgcaat acgactttcg cctggtctgc gccacagggg ccgttttgtt tgtgctatgt
    43921 gcggcgttca atgcgtggtt gttaccggca tggaaactct ccaccgtacg cacgcccgtt
    43981 cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt cctgacgctg
    44041 gcgggttact acatgctggc tgtacaagtg atgctgatgc tgccaattat ggtcaacgac
    44101 gtggctggcg cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg tctgtcgtta
    44161 acgttgctct accctatcgc ccgctggagt gaaaagcatt ttcgtctgga acaccggttg
    44221 atggctgggc tgttgataat gtcattaagc atgatgccgg tgggcatggt cagcggcctg
    44281 caacaacttt tcaccctgat ttgtctgttt tatatcgggt cgatcattgc cgagcctgcg
    44341 cgtgaaacct taagtgcttc gctggcggac gcaagagctc gcggcagcta tatggggttt
    44401 agccgtctgg gtctggcgat tggcggcgct attggttata tcggtggtgg ctggctgttt
    44461 gacctgggca aatcggcgca ccagccagag cttccgtgga tgatgctggg aattattggc
    44521 atcttcactt tccttgcgct gggttggcag tttagtcaga aacgcgccgc gcgtcgtttg
    44581 ctggaacgcg acgcctgatt tgatactccc ccctgactcc tccatactga aagtgaactg
    44641 acagccaaat ggaggagtca cgtgaagcta tacatttacg atcactgccc ttactgcctc
    44701 aaagcccgca tgattttcgg cctgaaaaat atccccgtcg aattacatgt tctgctcaac
    44761 gacgacgcag aaacacccac ccggatggtc ggtcaaaaac aggttcccat tctgcaaaaa
    44821 gatgacagcc gctatatgcc agaaagcatg gatatcgttc actatgtcga taaactcgac
    44881 ggcaaaccgt tactgaccgg caaacgttcc cctgccattg aagagtggct gcgcaaggtc
    44941 aatggctacg ccaacaaact gctgttgccg cgttttgcca aatcggcatt tgatgagttt
    45001 tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag aggccagcgc gggtaatttt
    45061 gccgacctgc tggcccactc tgacggtctg attaagaata tcagcgatga tttgcgtgcg
    45121 ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga agatgatatt
    45181 cagctatttc cgctactgcg taatctgacg ctggtagccg gaatcaactg gccaagccgc
    45241 gttgctgatt accgcgataa tatggcgaaa cagacacaaa tcaatttgtt atcatcaatg
    45301 gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg ggttgccgcc gttttcagtt
    45361 ttttctgctg gcgagtttat cgctggtgac acatgctgcg ttgtgtcagg atattgacgc
    45421 agatttcatg tcgaggacta ccatgaacaa gtttttattt gccgctgcat tgatcgtcag
    45481 tggcctgctc gttggctgta atcaactcac ccaatacacc atcaccgaac aagaaattaa
    45541 ccagtcgctt gcgaaacata ataatttctc aaaagatatc ggtttacccg gcgtggctga
    45601 cgcccatatt gttctgacaa acttgaccag ccaaattggt cgcgaagagc cgaataaggt
    45661 taccctaacc ggagacgcca atctggacat gaactccctg ttcggtagtc agaaagcgac
    45721 catgaaactg aagctgaaag cgctgcccgt gtttgataaa gaaaaaggtg cgatcttcct
    45781 gaaagagatg gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa cggtgatgca
    45841 aacgttgctt ccctatttga accaggcatt acgcaattac tttaaccagc aacctgctta
    45901 cgtcctgcgc gaagatggca gtcagggcga agcaatggcg aaaaaactgg cgaaaggcat
    45961 tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat taatgacgag ggcgcattcg
    46021 cgccctttat ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt ccggcaaaaa
    46081 catcccttca gccggagcat agagattaat gactgcacca tcccaggtat taaagatccg
    46141 ccgcccagac gactggcacc ttcacctccg cgatggcgac atgttaaaaa ctgtcgtgcc
    46201 gtataccagc gaaatttatg gacgggctat cgtaatgccc aatctggctc cgcccgtgac
    46261 cacagttgag gctgccgtgg cgtatcgcca acgtattctt gacgccgtac ctgccgggca
    46321 cgatttcacc ccattgatga cctgttattt aacagattcg ctggatccta atgagctgga
    46381 gcgcggattt aatgaaggcg tgttcaccgc tgcaaaactt tacccggcaa acgcaaccac
    46441 taactccagc cacggcgtga cgtcaattga cgcaatcatg ccggtacttg agcgcatgga
    46501 aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca catgcagata tcgacatttt
    46561 tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct ctgcgccagc gcctgactgc
    46621 gctgaaagtc gtttttgagc acatcaccac caaagatgct gccgactatg tccgtgacgg
    46681 aaatgaacgg ctggctgcca ccatcactcc gcagcatctg atgtttaacc gcaaccatat
    46741 gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc atcctcaaac gcaatattca
    46801 ccaacaggca ttgcgtgaac tggtcgccag cggttttaat cgagtattcc tcggtacgga
    46861 ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc ggctgcgcgg gctgcttcaa
    46921 cgccccaacc gcgctgggca gttacgctac cgtctttgaa gagatgaatg ctctgcagca
    46981 ctttgaagca ttctgttctg taaacggccc gcagttctat ggcttgccgg tcaacgacac
    47041 attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa agcatcgcac tgactgatga
    47101 caccctggtg ccattcctcg ccggggaaac ggtacgctgg tccgttaaac aataaaaatc
    47161 gtagcttcct gttgtcatta ggttatttta cctgtataaa taaccagtat attcaacagg
    47221 gggctattat gcgaattgaa gtcaccatag cgaaaacttc tccattgcca gctgg
//
LOCUS       DADLSB010000013        47036 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.013, whole genome shotgun sequence.
ACCESSION   DADLSB010000013 DADLSB010000000
VERSION     DADLSB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.013"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JN331_000756"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JN331_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY7258975.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLCFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1958)
                     /locus_tag="JN331_000757"
     CDS             complement(1614..1958)
                     /locus_tag="JN331_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY7258976.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAM
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGTSKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN331_000758"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN331_000758"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY7258977.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN331_000759"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN331_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY7258978.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /locus_tag="JN331_000760"
     CDS             complement(6144..6842)
                     /locus_tag="JN331_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY7258979.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN331_000761"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN331_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY7258980.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JN331_000762"
     CDS             7537..8574
                     /locus_tag="JN331_000762"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7258981.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMVANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN331_000763"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN331_000763"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY7258982.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JN331_000764"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JN331_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY7258983.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN331_000765"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN331_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY7258984.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JN331_000766"
     CDS             10745..11626
                     /locus_tag="JN331_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7258985.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12989)
                     /gene="srmB"
                     /locus_tag="JN331_000767"
     CDS             complement(11661..12989)
                     /gene="srmB"
                     /locus_tag="JN331_000767"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY7258986.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13121..13858
                     /gene="trmN"
                     /locus_tag="JN331_000768"
     CDS             13121..13858
                     /gene="trmN"
                     /locus_tag="JN331_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY7258987.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13843..15465)
                     /gene="nadB"
                     /locus_tag="JN331_000769"
     CDS             complement(13843..15465)
                     /gene="nadB"
                     /locus_tag="JN331_000769"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY7258988.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15721..15876
                     /gene="rseD"
                     /locus_tag="JN331_000770"
     CDS             15721..15876
                     /gene="rseD"
                     /locus_tag="JN331_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY7258989.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWCFDSAWKFG
                     LGRLYLG"
     gene            15873..16448
                     /gene="rpoE"
                     /locus_tag="JN331_000771"
     CDS             15873..16448
                     /gene="rpoE"
                     /locus_tag="JN331_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY7258990.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16481..17131
                     /gene="rseA"
                     /locus_tag="JN331_000772"
     CDS             16481..17131
                     /gene="rseA"
                     /locus_tag="JN331_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY7258991.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17131..18087
                     /gene="rseB"
                     /locus_tag="JN331_000773"
     CDS             17131..18087
                     /gene="rseB"
                     /locus_tag="JN331_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY7258992.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18084..18563
                     /gene="rseC"
                     /locus_tag="JN331_000774"
     CDS             18084..18563
                     /gene="rseC"
                     /locus_tag="JN331_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY7258993.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18761..20560
                     /gene="lepA"
                     /locus_tag="JN331_000775"
     CDS             18761..20560
                     /gene="lepA"
                     /locus_tag="JN331_000775"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY7258994.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20576..21550
                     /gene="lepB"
                     /locus_tag="JN331_000776"
     CDS             20576..21550
                     /gene="lepB"
                     /locus_tag="JN331_000776"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY7258995.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21822..22502
                     /gene="rnc"
                     /locus_tag="JN331_000777"
     CDS             21822..22502
                     /gene="rnc"
                     /locus_tag="JN331_000777"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY7258996.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22499..23404
                     /gene="era"
                     /locus_tag="JN331_000778"
     CDS             22499..23404
                     /gene="era"
                     /locus_tag="JN331_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY7258997.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPIILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23416..24144
                     /gene="recO"
                     /locus_tag="JN331_000779"
     CDS             23416..24144
                     /gene="recO"
                     /locus_tag="JN331_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY7258998.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24156..24887
                     /gene="pdxJ"
                     /locus_tag="JN331_000780"
     CDS             24156..24887
                     /gene="pdxJ"
                     /locus_tag="JN331_000780"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY7258999.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVDAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24887..25267
                     /locus_tag="JN331_000781"
     CDS             24887..25267
                     /locus_tag="JN331_000781"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY7259000.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25687..25767
                     /gene="shoB"
                     /locus_tag="JN331_000782"
     CDS             25687..25767
                     /gene="shoB"
                     /locus_tag="JN331_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY7259001.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFS"
     gene            complement(25961..26221)
                     /gene="yfhL"
                     /locus_tag="JN331_000783"
     CDS             complement(25961..26221)
                     /gene="yfhL"
                     /locus_tag="JN331_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY7259002.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(26277..27125)
                     /locus_tag="JN331_000784"
     CDS             complement(26277..27125)
                     /locus_tag="JN331_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7259003.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            27334..27969
                     /gene="yfhb"
                     /locus_tag="JN331_000785"
     CDS             27334..27969
                     /gene="yfhb"
                     /locus_tag="JN331_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY7259004.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            27994..28530
                     /gene="tadA"
                     /locus_tag="JN331_000786"
     CDS             27994..28530
                     /gene="tadA"
                     /locus_tag="JN331_000786"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY7259005.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(28527..30083)
                     /gene="mltF"
                     /locus_tag="JN331_000787"
     CDS             complement(28527..30083)
                     /gene="mltF"
                     /locus_tag="JN331_000787"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY7259006.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYLIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            30341..34228
                     /gene="purL"
                     /locus_tag="JN331_000788"
                     /gene_synonym="purI"
     CDS             30341..34228
                     /gene="purL"
                     /locus_tag="JN331_000788"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY7259007.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            34804..36231
                     /gene="qseE"
                     /locus_tag="JN331_000789"
     CDS             34804..36231
                     /gene="qseE"
                     /locus_tag="JN331_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY7259008.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSISFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            36396..37109
                     /gene="qseG"
                     /locus_tag="JN331_000790"
     CDS             36396..37109
                     /gene="qseG"
                     /locus_tag="JN331_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY7259009.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            37099..38433
                     /gene="glrR"
                     /locus_tag="JN331_000791"
     CDS             37099..38433
                     /gene="glrR"
                     /locus_tag="JN331_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY7259010.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            38494..38832
                     /gene="glnB"
                     /locus_tag="JN331_000792"
     CDS             38494..38832
                     /gene="glnB"
                     /locus_tag="JN331_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY7259011.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(38877..40067)
                     /gene="hmpA"
                     /locus_tag="JN331_000793"
     CDS             complement(38877..40067)
                     /gene="hmpA"
                     /locus_tag="JN331_000793"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY7259012.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            40394..41647
                     /locus_tag="JN331_000794"
     CDS             40394..41647
                     /locus_tag="JN331_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY7259013.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(41845..43038)
                     /locus_tag="JN331_000795"
     CDS             complement(41845..43038)
                     /locus_tag="JN331_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY7259014.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGELWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            43336..46431
                     /locus_tag="JN331_000796"
     CDS             43336..46431
                     /locus_tag="JN331_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAY7259015.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVANH
                     HIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKRFE
                     QYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNALLD
                     DAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPLAA
                     QDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRADFP
                     QAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSKAG
                     GYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQREK
                     LLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAAEM
                     LNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEECW
                     FARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAARYL
                     DNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLWHL
                     TGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHAAL
                     HYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYYND
                     QPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSLYG
                     DLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFNYV
                     H"
     gene            46528..>47036
                     /locus_tag="JN331_000797"
     CDS             46528..>47036
                     /locus_tag="JN331_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7259016.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINC"
BASE COUNT        11348 a        11855 c        12498 g        11335 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctctgttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgtccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagcatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtctgcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatctcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggctag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga cggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggta
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa attatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggctgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttagctt ttttcttctc ggcgcgtttt gccagtactt tcttcgatgg cttgccggtc
    11821 tgcttctcgc ttggcgcacg cgttttcggg cgtaactcat caataacgcg agctttaatc
    11881 ggctcttcaa tgtaacggcc aaccttgccc agcagcaaat ggtcatgggc ttccaccagc
    11941 gaaattgcgg tgcctttacg accggcgcgc gcggtacgtc cgatacgatg caaataagta
    12001 tcgccactgc gcggcatatc gaagttaaag acgtggctga cgtcaggaat gtcgataccg
    12061 cgcgcggcaa catcggtggc aaccagtacg tttacgcggc cttcggtcag gcgtttaatt
    12121 gcttcgttac gcttgccttg taccatctca ccttcgagat agcagttgtt gatgcccgct
    12181 tcgcgcagcc agtttgccag ctcatgcaca cgttcacgct tacgcacaaa cacgattgag
    12241 cgagtcgctt caggctgttt taacaggtgc accagcaatg cggttttatg ttccaggtca
    12301 tcggcgcggt aataccactg atgaattttt ttgcgctcac gggtggacgg attggcagaa
    12361 acttccaccg gatcttccag cagacgctcg gcaaagtcct gaatcgcatc gccttccagc
    12421 gttgccgaaa agagcagggt ctgtttacgc cagcgagttt cgccagcaat atgttcgata
    12481 tcctgagcga agcccatatc cagcatacgg tctgcttcgt cgaggatcag cgtttcaacc
    12541 gcgcggcaat cgaagttctc ttcttttatg tattgcagca gacgtccggt cgtggcgacc
    12601 acgatgtcct gattttcgct gaacacttcc gcgtggttca tataggctac gccgccggtg
    12661 atggtggcga tatccagatg cgtatgtttc gccagttcgc gggcatgatc ggccacctgc
    12721 atcgccagct cgcgagttgg ggtgaggatc aaaatacgcg gcggaccgga tttcttacgt
    12781 gggaaatcga gcaggtgctg caacgctggc agcagatacg ccgccgtttt accggtgcct
    12841 gtcggcgcag aaccgagtac atcacggcca tcgagcgcag gcggaatggc ggcagcctga
    12901 atggcggtcg ggcgagtgaa acctttatcc tggagggctt ccagcaggct ttcgtcgagt
    12961 tcaagttcgg aaaaagtcgt tacagtcatg ttctacctct gtgtggggcg ctgattatag
    13021 acgttacggc tgcaatcttc atctgtttgt atggatatcg cttctcgggt attgttctca
    13081 cccggtgcta tcgccgtttt tcctgcaagg ttttattttc atgtcacagt ctacatccgt
    13141 gcttcgtcgt aatggattta cttttaaaca gttttttgtt gctcacgatc gctgtgcgat
    13201 gaaagtggga acggatggca ttttgctggg cgcatgggca ccggtggctg gggtgaaacg
    13261 ttgccttgat atcggcgcgg gtagcgggtt gctggcactg atgctggcgc agcgaaccag
    13321 tgacagcgtg ataattgatg ccgttgagct ggaaagtgaa gctgccgcgc aggcgcagga
    13381 aaatatcaac cagtccccgt gggcagagcg gattaacgtc catacggcgg atattcagca
    13441 gtggatcaca cagcagacag tacgcttcga tttaatcatc agtaacccac cttactatca
    13501 gcagggagta gagtgcgcga cacctcaacg ggaacaggct cgctatacca ctacgcttga
    13561 tcacccatcg ctgctgacct gtgcggcgga gtgcattacc gaagagggat ttttctgcgt
    13621 ggtgctgcca gagcagattg gtaatggttt tactgaactg gcattaagca tgggctggca
    13681 tttacgtttg cggacggatg tggcggaaaa cgaagcgcga ctgccgcatc gggtgctgct
    13741 ggcattctcc ccgcaggcgg gagaatgctt tagcgatcgc ttagtcattc gtgggccaga
    13801 ccagaactat tccgaagcgt atacggcgct gacccaagct ttttatctgt ttatgtaatg
    13861 attgccgggg gaaaggatcg atggaccgga atgcgtgagc agttccgggt aatccagcgt
    13921 aaaatgcagc ccccggcttt ctttgcgcat catcgcgcag cgaacaatca actcggcaac
    13981 ctgtaccaga ttacgcagct ccagcaaatt atttgagacg cggaaatggg cgtaatattc
    14041 gtctatttct tgttggagca tggttatccg ccgcagggcg cgttccaggc gcttcgttgt
    14101 gcgcacaatg ccaacgtaat cccacataaa cagccgtagc tcgtgccagt tatgctgaat
    14161 tactacccgt tcgtcagggt tctcaacgcg gctttcatcc cacggcggta acgtactgac
    14221 gccgtgggca taaggcatac gtctggtgat atcttctgcc gccgaccagc catagaccag
    14281 acactccagc aatgaattcg aggccatgcg gttagcgccg tgtaagccgg tataactcac
    14341 ctcgccaatg gcatacaagc cctcgacgtc cgtacgccca tgatcatcaa ccattacacc
    14401 accgcaggta tagtgtgcag caggcacaat cggtaccggt tcttgtgtga gatcaatccc
    14461 cagcccgagc aatttttcat aaatcatcgg gaaatgctgg cgaataaaat cggcgggctt
    14521 atggctgatg tcgaggaaca tacaatctgc gccgaggcgt ttcatttcat ggtcaatggc
    14581 gcgggcgaca atatcgcgcg gggctagttc gccgcgctca tcaaaatcgg gcataaaacg
    14641 cgtgccatcc gggcgcttga gataagcgcc ttcgccgcgc agtgcttccg ttaacaggaa
    14701 attgcgtgcc tgtgggtgat acagtgcggt agggtggaac tgattaaatt cgagattggc
    14761 aacccgacag cccgcgcgcc acgccatagc aatgccatcg ccagaagaaa tatccggatt
    14821 ggtggtgtac tgatacacct tcgacgcacc gccggttgcc agtaccactg cttttgcgtg
    14881 gcaggtttcc accgtttctt tatttcggtt ccatacccac gcgcctacaa cccgtcgcgt
    14941 gcccggcagg ccaattttgt cagagacaat cagatcaact gcgttgctgc gctccagcac
    15001 acgaatattc ggatggttct gcgccttgct caccagcgtg gattgtactt ctctaccggt
    15061 ggcgtcggcg gcatgaagaa tacgacggtg actatgtcca ccttcacggg tcagatggta
    15121 gctttcttcg ccattcggtt gaacgtgggt atcaaacaaa accccctggt caattaacca
    15181 ttgcacacag gaacgagcat tgctggcgac aaattcaact gcatggcgat cgcaaatacc
    15241 agccccggca atcaatgtgt cttccacatg cgagtcaatg ctgtcagttt catcaaacac
    15301 ggcggcaata ccgccctggg cataaaatgt tgaaccttcc gttaccgggc ctttacttag
    15361 aacgatgacc tgatgctggt cagccaggcg tagcgccagt gaaagtccgg ctgcgccgct
    15421 accaataatc aacacgtcac atgaatgttc agggagagta ttcattttct ttgtttaatt
    15481 tactaaacat tgtttggtca gcatagcatc atgttgtgcg aataaacacc tgctatttta
    15541 atatttgtta cagtggctaa acacgttgac gcagggcggc gagaaaaaga gaagttactg
    15601 gctggtggag gattaggtgg tgaaataaaa aggccgttgg gttactcttc aggcagttaa
    15661 atgggcattt ctacacagat aatgcgatgt tcagattctg tagacttata atgatagata
    15721 atgatccgtc tacagcatga caaacaaaaa cagatgcgtt acggaacttt acaaaaacga
    15781 gacactctaa ccctttgctt gctcaaattg cagctaatgg agtggtgttt cgatagcgcg
    15841 tggaaatttg gtttggggag actttacctc ggatgagcga gcagttaacg gaccaggtcc
    15901 tggttgaacg ggtccagaag ggagatcaga aagcctttaa cttactggta gtgcgctatc
    15961 agcataaagt ggcgagtctg gtttcccgct atgtgccgtc gggtgatgtt cccgatgtgg
    16021 tacaagaagc ttttattaaa gcctatcgtg cgctggattc gttccgggga gatagcgctt
    16081 tttatacatg gctgtatcgg attgctgtaa atacagcgaa aaattacctg gttgctcagg
    16141 ggcgtcgtcc accttccagt gatgtggatg ccattgaagc tgaaaacttc gaaagtggcg
    16201 gcgcgttgaa agaaatttcg aaccctgaga acttaatgtt gtcagaagaa ctgagacaga
    16261 tagttttccg aactattgag tccctcccgg aagatttgcg catggcaata accttgcggg
    16321 agctggatgg cctgagctat gaagagatag ccgctatcat ggattgtccg gtaggtacgg
    16381 tgcgttcacg tatcttccga gcgagggaag ctattgataa caaagttcaa ccgcttatca
    16441 ggcgttgacg atagcgggat actggataag ggtattaggc atgcagaaag aacaactttc
    16501 cgctttaatg gatggcgaaa cgctggatag tgagctgctt aacgaactgg ctcataaccc
    16561 agaaatgcag aaaacctggg aaagctatca cttaatccgt gattcaatgc ggggtgatac
    16621 tcccgaggtg ctccatttcg atatctcttc acgcgtgatg gccgccattg aagaagagcc
    16681 agtacgtcaa ccggcgacat tgatcccgga agcccagcct gcgccgcatc aatggcagaa
    16741 aatgccattc tggcagaaag tacgtccgtg ggcggcacag cttacccaaa tgggcgtagc
    16801 cgcatgcgta tcgcttgcag ttatcgttgg cgtccagcac tataatggac aatctgaaac
    16861 gtcccagcag cccgaaacgc cggtatttaa tacactgccg atgatgggta aagccagccc
    16921 ggtaagcctg ggagtacctt ctgaagcgac cgcaaacaat ggtcaacagc agcaggtaca
    16981 ggagcagcgt cgtcgcatta atgcaatgtt gcaggattac gaactgcaac gccgactcca
    17041 ctctgaacag cttcagtttg agcaggcgca aacccagcaa gccgctgtac aggtgccagg
    17101 aattcaaact ttaggaacgc aatcgcagta atgaagcaac tttggtttgc catgtcatta
    17161 gtgacaggta gcctgttatt ctctgctaac gcctcggcca ctcccgcgtc cggggcgtta
    17221 ttacagcaga tgaacctggc cagtcagtca ctgaattacg agctgtcatt catcagcatc
    17281 aataaacagg gtgttgagtc tctgcgttat cgacatgcac gcctcgataa ccgtcctctt
    17341 gcacaattgt tgcaaatgga tggcccgcgc cgggaagtgg tacagcgcgg caatgaaatc
    17401 agctattttg aaccgggact tgaaccgttc acgcttaatg gcgattacat tgttgattct
    17461 ctgccatcgc ttatctatac cgatttcaaa cgcctttctc cttactacga ctttatctcc
    17521 gtcgggcgca cgcgtattgc tgatcgtctt tgcgaagtca ttcgcgtggt tgcccgagat
    17581 ggtacacgct acagctacat cgtgtggatg gacaccgaat cgaaattacc gatgcgggtt
    17641 gatctccttg atcgcgatgg tgaaacgctg gaacaatttc gcgtgattgc ttttaacgtc
    17701 aatcaggata tcagcagcag tatgcagacg ctggcgaagg caaatttgcc gccgttgctt
    17761 tctgttcctg taggtgaaaa agctaaattc agctggacgc caacctggtt gccacagggt
    17821 tttagcgaag tttccagtag tcgacgtccg ctaccgacga tggacaacat gcctatcgaa
    17881 tcacgtctct attccgacgg attattcagc ttctcggtaa acgttaaccg cgctacgcca
    17941 tcgagcaccg atcagatgtt gcgcaccgga cgcagaaccg tcagtacaag cgtacgtgat
    18001 aacgccgaaa tcaccattgt cggtgaactg ccgccgcaaa cggcgaaacg cattgccgag
    18061 aatattaagt tcggggcagc gcaatgatca aagagtgggc taccgtcgtc tcctggcaaa
    18121 acgggcaggc gctggtcagt tgtgatgtta aagcttcatg cagcagctgc gcttcacgcg
    18181 ccggttgcgg tagccgcgtg ttgaataaac ttggcccgca aaccacgcat accattgtcg
    18241 taccctgtga tgagccgtta gtgccggggc aaaaagtgga attggggatc gccgaaggca
    18301 gcctgcttag ctccgcatta ctggtttata tgtcgccgct ggtgggatta ttcctcatcg
    18361 cttcgctatt tcagctactc tttgcttctg acgtcgcagc attatgtggt gcggtcctcg
    18421 gcggtattgg cgggttcctg attgcgcgcg gctactcgcg taagtttgct gcccgggcag
    18481 aatggcaacc gatcatctta agcgtggcat tgccgcctgg ccttgtgcga tttgaaacat
    18541 cttcggaaga cgcgagccag tgatttcact ggctttgttg cattttgcat aacaaatgcg
    18601 aggacgtttc cagataccac aatccatacg gtatgaacat ttcctcgcct caatgttgta
    18661 gtgtagaatg cggcgtttct attaatacag acgttaagct cagaacagcg acttctaaaa
    18721 gcctggttaa ccgggcatta aggcacaata atcatacttt atgaagaata tacgtaactt
    18781 ttcgatcata gctcacattg accacggtaa atcgacgctg tctgaccgta ttatccagat
    18841 ctgcggtggc ctgtctgacc gtgaaatgga ggcgcaggtt ctcgattcca tggatcttga
    18901 gcgtgagcgt ggcattacca tcaaagcgca aagcgtgacg ctggactaca aagcgtctga
    18961 cggcgaaacc tatcagctta actttatcga caccccgggc cacgtagact tctcctatga
    19021 agtttcccgt tcgctggctg cctgtgaagg tgcattgctg gtggtcgacg ccgggcaggg
    19081 cgtagaagcg caaaccttgg caaactgcta caccgccatg gaaatggatc tcgaagtggt
    19141 gccggtactg aacaagattg acctgccggc agccgatcct gaacgcgtgg cggaagaaat
    19201 tgaagatatc gtcggcatcg acgccaccga tgcagtgcgc tgttcagcga aaaccggcgt
    19261 tggcgtgcag gacgttctcg aacgtctggt gcgcgacatt ccgccgccgg aaggcgatcc
    19321 ggaaggcccg ttgcaggcac taattatcga ctcctggttc gacaactacc tgggcgttgt
    19381 ttcacttatc cgtattaaaa acggcaccct gcgtaagggc gacaaagtga aagtcatgag
    19441 taccgggcag acctataacg ccgaccgtct gggcatcttc acgccgaaac aggttgaccg
    19501 cactgaactg aaatgtggcg aagtaggctg gctcgtatgt gcgattaaag atatccacgg
    19561 cgctccagtc ggcgatacct taacgctggc gcgtaatccg gcagaaaagg cgctgcctgg
    19621 ctttaagaaa gtcaaaccgc aggtatacgc cggtctgttc ccggtaagtt ccgacgacta
    19681 tgaagccttc cgtgacgcgc tgggtaaact cagcctgaac gatgcctcac tgttctatga
    19741 gccggaaagc tccagcgcgc tgggctttgg tttccgctgc ggcttccttg gcctgctgca
    19801 catggagatc atccaggagc gtctggaacg tgaatacgat ctggatctga tcaccactgc
    19861 gccgaccgta gtgtatgaag ttgaaaccac gtcaagggaa gttatctacg tcgacagccc
    19921 atccaagctg cctgcggtaa ataacattta cgaactgcgc gagccgattg cagagtgtca
    19981 catgctgctg ccgcaggcat atctcggcaa cgttattacg ctgtgcgtag aaaaacgcgg
    20041 cgtgcagacc aatatggttt accacggtaa tcaggtggcg ctgacgtacg agatcccgat
    20101 ggcggaagtg gtgctcgact tcttcgatcg cctgaaatct acctcgcgtg gttatgcgtc
    20161 tctggattac aacttcaaac gcttccaggc gtccgacatg gtacgtgtag acgtattaat
    20221 caacggtgaa cgtgttgatg cgctggcgtt gatcacccac cgtgataatt cgcaaaaccg
    20281 cggtcgcgag ttggtggaga agatgaaaga tctgatccca cgccagcagt ttgatatcgc
    20341 cattcaggca gcgattggta cgcacattat tgcgcgatcc accgtgaaac agctgcgtaa
    20401 aaacgtactg gctaaatgtt atggcggcga tatcagccgt aagaaaaagc tgctgcagaa
    20461 gcagaaagaa ggtaagaaac gcatgaagca gatcggtaac gttgagctgc cgcaggaagc
    20521 gttcctcgcc attctgcacg tcggcaaaga caacaaataa cccttaggag ttggcatggc
    20581 gaatatgttt gccctgattc tggtgattgc cacactggtg acgggcattt tatggtgcgt
    20641 ggataaattc tttttcgcac ctaaacggcg ggaacgtcag gcagcggcgc aggcggctgc
    20701 cggtgactca ctggataaag caacgttgaa aaaggttgca ccgaagcctg gctggctgga
    20761 aaccggagct tctgtttttc cggtgctggc tatcgtattg attgtacgtt cgtttattta
    20821 tgaaccgttc cagatcccgt caggttcgat gatgccgact ctgttaatcg gtgattttat
    20881 tctggtagag aagtttgctt atggcattaa agatcctatc taccagaaaa cgctgatcga
    20941 aaccggtcat ccgaaacgcg gcgatatcgt ggtctttaaa tatccggaag atccaaagct
    21001 tgattacatc aagcgcgcgg tgggtttacc gggcgataaa gtcacttacg atccggtctc
    21061 aaaagagctg acgattcaac cgggatgcag ttccggccag gcgtgtgaaa acgcgctgcc
    21121 ggtcacctac tcaaacgtgg aaccgagcga tttcgttcag accttctcac gccgtaatgg
    21181 tggggaagcg accagcggat tctttgaagt gccgaaaaac gaaaccaaag aaaatggaat
    21241 tcgtctttcc gagcgtaaag agacactggg tgatgtgacg caccgaattc tgacagtgcc
    21301 gattgcgcag gaccaggtgg ggatgtatta ccagcagcca gggcaacaac tggcaacctg
    21361 gattgttccg ccgggacaat acttcatgat gggcgacaac cgcgacaaca gcgcggacag
    21421 ccgttactgg ggctttgtgc ctgaagcgaa tctggtcggt cgggccacgg ctatctggat
    21481 gagcttcgat aagcaagaag gcgaatggcc gactggtgtg cgcttaagtc gcattggcgg
    21541 catccattaa tagccatctt cgttcacgtt gtcgccgtta tggcgacaac gtgaattatt
    21601 tatgagataa atctcccgtg gctaacgaca tcccccgtcg ttgtgtatag aatattcccc
    21661 cgaagtttaa ggttggcacc tccaggttgc cacggcacac gaaacagcgt tggtccccat
    21721 ataccgctaa actgaaactg cagcgaagca gttagcagag ccatgtatat caggtctgtt
    21781 tcgtgtgctg aattgttgac gcatttattt attggtatcg catgaacccc atcgtaatta
    21841 atcggcttca acggaagctg ggctacactt ttaatcatca ggaactgttg cagcaggcat
    21901 taactcatcg tagtgccagc agcaaacata acgagcgttt agaattttta ggcgactcta
    21961 ttctgagcta cgttatcgcc aatgcgcttt atcaccgttt ccctcgtgtg gatgaaggcg
    22021 atatgagccg gatgcgcgcc acgctggtcc gtggcaatac gctggcggaa ctggcgcgcg
    22081 aatttgagtt aggcgagtgc ttacgtttag ggccaggtga acttaaaagc ggtggatttc
    22141 gtcgtgagtc aattctcgcc gacaccgtcg aagcattaat tggtggcgta ttcctcgaca
    22201 gtgatattca aaccgtcgag aaattaatcc ttaactggta tcaaactcgt ctggacgaaa
    22261 ttagcccagg cgataaacaa aaagatccga aaacgcgctt gcaagaatat ttgcagggtc
    22321 gccatctgcc gctgccgact tatctggtag tccaggtacg tggcgaagcg cacgatcagg
    22381 aatttactat ccactgccag gtcagcggcc tgagtgaacc ggtggttggc acaggttcaa
    22441 gccgtcgtaa ggctgagcag gctgccgccg aacaggcgtt gaaaaaactg gagctggaat
    22501 gagcatcgat aaaagttact gcggatttat tgccatcgtc ggacgtccga acgttggcaa
    22561 atccacattg ttgaacaaac tgctggggca gaaaatctcc atcacctccc gcaaggcgca
    22621 gacaactcgt caccgcattg tggggatcca tactgaaggc gcgtatcagg cgatctacgt
    22681 cgatacaccg ggcctgcata tggaagaaaa acgcgccatt aaccgcctga tgaacaaagc
    22741 ggcgagcagc tctattggcg atgttgagct ggtgattttt gtcgttgaag gcacccgctg
    22801 gacgccggac gacgaaatgg tgctcaacaa actgcgcgaa ggcaaagcgc cgataatcct
    22861 cgcggtgaac aaagtggaca acgtgcagga gaaagccgat ctgctgccgc acctgcagtt
    22921 cctggcaagc cagatgaact tcctcgatat cgtgccaatc tctgccgaaa ccgggctgaa
    22981 tgttgacact attgcggcaa tcgtgcgtaa gcatctgcct gaagcgactc atcacttccc
    23041 ggaagattac atcaccgatc gctcacagcg ttttatggcg tctgaaatca tccgcgaaaa
    23101 actgatgcgt ttccttggcg ctgaactgcc gtactccgtg accgtggaga tcgaacgttt
    23161 cgtctctaac gaacgcggcg gttatgacat caacggtttg attctcgttg agcgtgaagg
    23221 gcagaagaag atggttattg gcaacaaagg ggccaagatc aaaaccatcg ggattgaagc
    23281 gcgtaaagac atgcaggaaa tgttcgaagc gcctgtccac cttgaactgt gggtaaaagt
    23341 gaaatcgggt tgggccgacg acgaacgcgc actgcgcagt ctcggttatg ttgacgatct
    23401 ttaagagtca ctccgatgga aggctggcag cgcgcatttg tcctgcatag tcgcccgtgg
    23461 agcgaaacca gcctgatgct ggacgtcttc acagaggaat cgggtcgcgt gcgtctggtt
    23521 gccaaaggcg cacgctctaa acgctctact ctgaaaggtg cattacagcc tttcacccct
    23581 ctcttgctac gttttggcgg gcgtggcgaa gtcaaaacgc tgcgcagtgc tgaagctgtc
    23641 tcactggcgc tgccattaag cggtatcacg ctttacagcg gtctgtacat caacgaactt
    23701 ctctcccgcg tactggaata cgagacgcgc ttctctgaac tctttttcga ttacttgcac
    23761 tgcattcagt ctcttgcagg tgccactggt acgccagaac ccgcgctgcg ccgctttgaa
    23821 ctggcactac tcgggcatct gggctatggc gtcaatttta cccattgcgc gggtagcggc
    23881 gagccggtag atgacaccat gacgtatcgt tatcgcgaag aaaaagggtt tatcgcaagc
    23941 gtcgttatcg acaataaaac gttcaccgga aggcagttaa aagcgttaaa cgcgcgggaa
    24001 tttcctgacg cagacacact acgcgccgcg aaacgcttta cccgaatggc gcttaagccg
    24061 tatcttggcg gtaaaccatt aaagagcagg gaactgttcc ggcagtttat gcctaagcga
    24121 acggtgaaaa cacattatga atgatgagga ttgtcatggc tgaattactg ttaggcgtca
    24181 acattgacca tattgcaacg ctgcgtaacg cacgcggtac tgcttacccg gatccggtgc
    24241 aggcggcgtt tattgccgag caggcgggag cggacggcat taccgtgcat ttacgtgaag
    24301 atcgccgcca cattactgac cgcgatgtgc gcatcctgcg gcagacgctg gatacccgaa
    24361 tgaatctgga gatggcagtg accgaagaga tgctggcgat cgccgttgag acgaagccac
    24421 atttttgctg cttggtaccg gaaaagcgtc aggaagtaac aaccgaaggc ggcctggacg
    24481 ttgcagggca gcgtgaaaaa atacgcgatg cctgcaaacg tctggcagat gccgggattc
    24541 aggtttctct gtttatcgac gccgatgaag agcagatcaa agctgcggca gaggttgacg
    24601 caccgtttat cgagatccac accggttgct atgctgatgc caaaactgac gccgaacagg
    24661 cgcaagagct ggtgcgtatc gccaaagccg cgacctttgc cgctagtctc ggtctgaaag
    24721 ttaacgccgg acacggtctg acctatcaca acgtgaaagc cattgccgcc atccctgaga
    24781 tgcatgaact gaatatcggt catgccatta ttggccgtgc agtgatgacc ggactgaaag
    24841 atgcggtggc agaaatgaag cgtctgatgc tggaagcgcg tggctaatgg caatattagg
    24901 tttaggcacg gatatcgtag agatcgctcg catagaagca gtgatcgccc gatccggtga
    24961 gcgcctggca cgccgcgtat taagcgataa cgaatgggca atctggaaaa cgcaccacca
    25021 gccggtgcgt tttctggcga agcgttttgc tgtgaaagaa gccgcagcaa aagcgtttgg
    25081 caccgggatc cgcaatggtc tggcgtttaa tcaatttgaa gtattcaatg atgagctcgg
    25141 caaaccacgg ctacggctat ggggtgaggc attaaaactg gcggaaaagc tcggcgttgt
    25201 aaatatgcat gtaacgctgg cagatgaacg ccactatgct tgtgccacgg taattattga
    25261 aagttaaaat aataccgttc tctttaaaaa gtaaaacccc cgaactggta aggaacgggg
    25321 gttttacttt ttaactttaa ttttgtgcag catgcaccct caacaacagc gattatggat
    25381 cagtgtactt atcttttaca agcaacttgc aatctttagc ataaaaactc gagcctttac
    25441 gaagaaagca atattgatgg aaagattaac gtgaccgcca attcgtaagt acattaaaat
    25501 tggcttcgtt attgaagatt ttgctgtgct ttacaccatg ccacagaatt cccccattga
    25561 aacgagtggt gtcgtcaaag ctctggtgtg gagtgcagca tgcaccctca ataactcgca
    25621 cgttcagttt tggggagatg taagggctaa tctgaatggc tgcattcctt gtttaaggaa
    25681 aaacgaatga ctgattgccg atacctgatt aaacgggtca tcaaaatcat cattgctgtt
    25741 ttacagctga tccttctgtt ctcataacac aaggaaacgt acttaaggtg cgtccggtga
    25801 accaatcgga cgcgccttta ataactataa ataagtgtct tggcagatac tatataaatt
    25861 aacttagtga atgattatgc taatgtcatc aattaaataa atataatggc gttaaggctt
    25921 cccagtaata taattaatac tctacttcca gagtagaata ttaaatttta tccgcgtggt
    25981 gcatcagcac aaatttatcc cacaactgtt cttctgtctc gacatgcgcc ggatctttca
    26041 caatagtatt ggggatcggg cacaccttct ggcaggttgg tgtctcgtag tgccctacgc
    26101 attcggtaca cttatcgctg ttaatctcgt agatatgatc tcccattgaa atcgcctcat
    26161 tcgggcattc gggttcacac atatcacaat tgatgcattt tttagtgatt aacagcgcca
    26221 taaggaaact ctcaaaacaa cacaaaccgg gcgggcatta tacgcgcatt ctttactcag
    26281 accagttttt tcaccagcgc ttcactatga cgaatacgtt ctggtgccag ttccagatcc
    26341 tgctgaatca gcgcaatgaa cagcaaatcc gttaacattc cctgagcgtg acaagcagag
    26401 attgaagcac tgtttgtcgc ttgttcttcg gcaatggtat acaggcaatg agaagcacgc
    26461 tgttgcaggg cattcggagt aaagccggta atcgccagca cttttccgcc cactcgcagc
    26521 atctcatctg ccgccaggtt taactcgcgt cgtacaccgg tgtaggaaat ggctaataac
    26581 agatcgtcag gggacgacgc ctgtactgtt gcgagtagcg catgcatatc gcgcagtgcg
    26641 gcagcattga agccaatctt catcagcttc caggcaaagt tttgcgccac cagacccgaa
    26701 gcgccaatac cggtcagaat tatccgccgc gcagagcgca acattgttac gcattcatgc
    26761 agtttctctt cactattaac gtttagcgtt gcgtacatag cggcagtatt ttctttaatc
    26821 agtttttcgc cgaccagccg taacggatca tcaccgcgga tttggttatg aatgggcacg
    26881 gagggcgatt ccggctggct tgccagcgct tcactcaacg ccaacttaag cgccggaaaa
    26941 cctttatagc cgagtttttg cgcgaacttc acgacgctgg actgactgac tccggcttcg
    27001 ttggccagtt gctgagagct taaatggcgc gccgtatcag gttgtagcag cagataatcc
    27061 gccagttttt tatcactctg ggcaagcccc tggtaacgct gacggatacg aagtaagccg
    27121 ttcatatctt tcctcgcgat gaaatagtgg cgacaacagg caatatgaga cacacgcgag
    27181 tgaattttat attccattgc cgctaattgc catgaattaa aaattccagt tagccatgat
    27241 gcggtcgatt gttgccggaa aagtgatgat ctatctgtta ggctatgtag tagcgaaaat
    27301 taatcaggat gtttcagtcc agaggagtat ggtttggcaa ctcacgagcg tcgtgtggtg
    27361 ttttttgact tagatggaac attgcatcag caggatatgt tcggcagttt tctgcgctat
    27421 ttactgcgtc gccaaccgct gaatgcgtta cttgtcctac cgttgttacc gattatagcc
    27481 attgcgttat tgataaaagg tcgtgcggca cgctggccga tgagtctgct tctgtggggg
    27541 tgcacttttg gtcacagcga agcacgttta cagacgttgc aggccgattt cgtgcgctgg
    27601 tttcgcgaca atgttaccgc ctttccgctg gttcaggagc gattaaccac ctacctgtta
    27661 agttccgatg ctgatatctg gttgattacc ggctctccgc agccgctggt tgaagcggtt
    27721 tatttcgata cgccctggct gccgcgggtt aatcttatcg ccagccaaat tcagcgtggc
    27781 tatggtggtt gggtattgac gatgcgttgt ctgggacatg aaaaggtcgc acaactggag
    27841 cgcaaaatcg gcacgccgct gcggctgtac agtggctata gcgacagtaa tcaggacaat
    27901 ccgctgcttt atttctgtca gcatcgttgg cgagtaaccc cgcgcggtga actccagcaa
    27961 ctggaataga gtaaagcata gcgtccgtgt ataatgcgcc gcgcttttat aaccggagtt
    28021 ttctttttgt ctgaagtcga atttagccac gaatactgga tgcgtcacgc gctgacgctg
    28081 gcgaaacgtg cctgggatga gcgggaagtg ccggtcggcg cggtattagt gcataacaat
    28141 cgggtaatcg gcgaaggctg gaaccgcccg attggtcgcc atgatcccac cgcacatgca
    28201 gaaatcatgg ccctgcggca gggtggtctg gtgatgcaaa attatcgtct gatcgacgcc
    28261 acgttgtatg tcacgcttga accatgtgta atgtgtgccg gagcgatgat ccacagtcgc
    28321 attggtcgcg tggtctttgg tgcgcgtgac gcgaaaactg gcgctgcggg atctttaatg
    28381 gatgtgctgc atcatccggg tatgaatcac cgagtggaaa ttacggaagg aatactggcg
    28441 gatgagtgcg cggcgttgct cagtgacttc tttcgcatgc gccgccagga aattaaagcg
    28501 cagaaaaaag cgcaatcctc gacggattaa ttttgtttct cttcactccc tttcctggaa
    28561 aacagcagag agggagaatg ggtcaaatag tttgatgacg actgggaaag aaacgagaga
    28621 aaaggaaatt tctctttgcc caactccgta ggcgataccg ccggataatc ctgcgccagt
    28681 tgcatcgccg cttctgtagc ctgcttctct ttctcttgca gataacccac caggctaatc
    28741 agatacttac gaatattttc gacataagcg taggcttcat gcccacgagc gtagccgtaa
    28801 gtcagcttgc tgtaataggg tttctggcta agtaaaggca gacgctgttt tacgtcagcc
    28861 caactgtcag gattcccttt ggtttttgcc gtcagggcgc gggcatccag catatgcgca
    28921 tagcccatat tgtacgcagc gagggcaaac cagatccgtt cgttctccgg cacactttcc
    28981 ggcactttac tcatcatatc ctgcaaataa cgcacgccac cgctgatgct ctgttcggca
    29041 tcggtacgat ccgtaatgcc gaggctttgc gcggtatttt tggttaacat catcatgccg
    29101 cgcacacccg tcggtgaagt ggcctgtgca tcccagtgcg attcctgata agcaatagcg
    29161 gccagcaaac gccagtcaat ttcttcggcg tatttctcaa acaggggctt taactgcggc
    29221 agtaccgcat cgacggcgcg taaaaatgtg cgcgtatcga cgtaatcaaa atcatcgcca
    29281 tgccccaggt atttctcttc aatgcgtgcc agcgtaccgt cttcattcat ttcgttgaag
    29341 aagtcgagca gggcggcgga aagggtatta tcgccatcta acgggctaaa ccaagtcacc
    29401 ggttgttcat cggtgatatc gagcgctacg gcgagctccg ggtgaacgcg ctgaaacagg
    29461 ctgatggcga cagaatcagc aatggtgtaa tcgagttttc cttcgatgac atcttccatt
    29521 aattccgcag agcctttttt gtcgtctacc ttccagctta attccgggaa ttttgtttct
    29581 ttcagggtct ggagatcgtt aaccaccaca tgacccggtg caacggtaag ttgctccgcc
    29641 gtcaggttgc ccagcgtacg tgggcgatac tgacccactt tataaaccag ttgttgtgac
    29701 acggaataat aggtagggcc aggctgataa ttttttaccc gctcactgtt atagaccagt
    29761 cctgccgcca gcaggtcggc gttaccatta tcaaggtcgt caaacagctg gctgatattc
    29821 tgccgcacgg tcactttcag ttttacgccg aggtaatcgg caaactgttt cgccagttcg
    29881 taatccaggc caaaaggttt cccgttgatt tcgttataag tcaggggagt atgaatggtg
    29941 ctcacacgca actctccccg cgcttgaatg gcggcgatac ggttgtcggc tttaccaaac
    30001 cagggaatgg atggccagag agcgaccgcg agcagcagtg ccagaatgcc gatgaacaga
    30061 taattaatct ttaatttttt caattagtta attctctgtg tcgtgcgcgt cccagcttga
    30121 aaaaacgtaa taatagtgaa aggtttactc ataaatgagc ggcattttgc gtaaacctgc
    30181 gccagatggc aacttattac agccattggc ggcacgcgtt gctaattcac gatggtgatt
    30241 ttatttccac gcaaacggtt tcgtcagcgc atcagattct ttataatgac gcccgtttcc
    30301 cccccttggg tacaccgaaa gcttagaaga cgagagactt atgatggaaa ttctgcgtgg
    30361 ttcgcctgca ctgtcggcat tccgaatcaa caaactgctg gcacgttttc aggctgccag
    30421 gctcccggtt cacaatattt acgccgagta tgtccatttt gctgacctca atgcgccgtt
    30481 aaacgatgat gagcacgcac aacttgaacg cctgctgaaa tatggtccgg cactcgccag
    30541 ccacgccccg caaggcaaac tcctgctggt gaccccgcgt cctggcacca tctccccctg
    30601 gtcttcgaaa gcgaccgata ttgcccataa ctgcgggcta caacaggtaa accgccttga
    30661 gcgcggcgtt gcttactata tagaagccgg tacgctgacc aatgaacaat ggcagcaggt
    30721 taccgctgaa ctgcacgacc gcatgatgga aacggtcttt tttgatttag atgatgcaga
    30781 gcagttgttt gcccaccatc aaccgactcc ggtcacaagc gttgatttgc tggggcaggg
    30841 ccgtcaggcg ctgatcgacg ctaacctgcg tcttggcctg gctctggcgg aagatgaaat
    30901 tgactatctg caggatgctt tcacaaagct tggtcgtaac ccgaacgaca tcgagctgta
    30961 tatgttcgct caggcgaact ctgagcactg ccgccacaaa atttttaacg ccgactggat
    31021 tatcgacggt gaacaacagc cgaaatcgct gttcaggatg atcaaaaata ctttcgaaac
    31081 cacgccagat cacgttctct ctgcttataa agataacgcc gccgtaatgg aaggttctga
    31141 agtgggccgc tactttgctg accacgaaac gggccgctac aatttccatc aggaaccggc
    31201 gcacatcctg atgaaagtcg aaactcacaa ccacccgacg gcgatttctc cgtggccggg
    31261 cgcggcgacc ggttccggcg gtgaaatccg cgatgaaggt gccaccgggc gcggcgcgaa
    31321 gccgaaagct ggtttggttg gtttctccgt atccaacctg cgaattcctg gcttcgaaca
    31381 gccgtgggaa gaagatttcg gtaagcctga gcgcattgtt accgcgctgg acatcatgac
    31441 cgaaggcccg ctgggcggtg cggcgtttaa caacgaattt ggtcgtccgg cactgaacgg
    31501 ctacttccgt acttatgaag aaaaagtgaa cagccacaat ggcgaagagc tgcgcggtta
    31561 tcacaaaccg atcatgctgg cgggcgggat cggcaacatt cgcgccgatc acgtacaaaa
    31621 aggcgagatc aacgtcggtg cgaagctggt cgttctcggc ggcccggcaa tgaacatcgg
    31681 tcttggcggc ggcgcggcgt cttccatggc gtctggtcag tctgatgccg acctcgactt
    31741 tgcttccgta cagcgcgaca acccggagat ggagcgtcgt tgccaggaag tgatcgaccg
    31801 ttgctggcag cttggtgatg ctaacccaat tctgtttatc cacgacgtcg gtgcgggcgg
    31861 tctttctaac gcgatgccgg agctggtgag cgacggcgga cgcggcggta aatttgaact
    31921 gcgcgatatt ctaagcgacg aaccgggcat gagcccgctg gaaatctggt gtaacgaatc
    31981 ccaggaacgc tacgtgctgg cggttgctgc cgatcaatta ccgctgtttg acgaactgtg
    32041 taagcgtgag cgcgcaccct acgcggtgat tggtgaagcg accgaagaac tgcatctttc
    32101 tctgcacgat cgtcattttg ataatcagcc gatcgatctg ccgctggacg tcctgcttgg
    32161 taaaacgccg aagatgaccc gcgatgtaca aacgctgaaa gcgaagggcg atgcgctggt
    32221 ccgtgaaggg atcaccattg cagacgcggt gaaacgcgta ctgcatctgc cgaccgtggc
    32281 ggagaaaact ttcttggtga ccatcggtga ccgcagcgta accggcatgg tggcgcgcga
    32341 tcagatggtg gggccgtggc aggtgccggt cgctaactgc gcggtcacta ccgccagcct
    32401 cgacagctac tacggtgaag cgatggcgat tggcgagcgt gcgccggttg cgctgctgga
    32461 tttcgccgcc tctgcccgtc tggcggtcgg tgaagcgtta accaatattg ccgcaacaca
    32521 gattggcgat atcaaacgca ttaaactttc cgccaactgg atggcggcgg caggccaccc
    32581 tggtgaagat gcgggcctgt atgaagccgt taaagccgtg ggtgaagaac tttgtccggc
    32641 gctgggcctg acgatcccgg tgggtaaaga ctctatgtcg atgaaaaccc gctggcagga
    32701 aggtaacgaa gagcgcgaaa tgacgtcgcc gctgtcgctg gtgatttctg catttgcccg
    32761 cgtggaagat gtacgtcaca ccatcacgcc gcagctttgt accgaagata acgcactgct
    32821 gctgattgat ttgggcaaag gcaataacgc gctgggcgca acggcgctgg cgcaggttta
    32881 tcgtcagctt ggcgacaaac cggcagatgt acgcgatgtt gcccaactga aaggcttcta
    32941 tgacgcgatt caggcgctgg ttgcacagcg taagctgctg gcgtatcacg accgctctga
    33001 tggcggcctg ctggtaacgc tggcggaaat ggcctttgct ggtcattgtg gcattgacgc
    33061 ggatatcgcc tctctgggtg acgatcgcct ggcggcgttg tttaacgaag aactgggtgc
    33121 ggtgattcag gttcgtgccg ctgaccgtga agcggtcgag tccgtactgg cacagcatgg
    33181 gcttgcggat tgcgtccatt atgttgggca ggcggtttcc ggtgaccgtt ttgtgattac
    33241 cgccaacggg cagactgtat tcagcgaaag ccgcactacg ttgcgtgtct ggtgggcaga
    33301 aactacctgg cagatgcagc gcctgcgtga caacccggag tgtgccgatc aggagcatca
    33361 ggcgaaatct aacgacgccg atccgggcct gaatgtaaaa ctgtcgttcg atatcaacga
    33421 agatgtggca gcaccgtata ttgccactgg cgcacgtccg aaagttgccg tactgcgtga
    33481 gcagggcgtg aactcgcatg ttgaaatggc ggcagctttc caccgtgggg gctttgatgc
    33541 tatcgacgtg catatgagcg acctgctgac tggacgtacg ggactggagg attttcacgc
    33601 cctggtcgcg tgcggaggtt tctcctacgg tgatgtgctg ggtgccggtg aaggttgggc
    33661 gaagtctatc ctgttcaatg accgtgtacg cgatgagttt gcaaccttct tccatcgtcc
    33721 gcaaacgctg gccctggggg tatgtaacgg ttgccagatg atgtctaacc tgcgtgaact
    33781 gatcccgggg agcgagttgt ggccacgttt tgtgcgtaac acctccgatc gctttgaagc
    33841 gcgtttcagc ctggttgaag taacccaaag cccgtctctg ctgttgcagg ggatggtggg
    33901 ctcgcaaatg ccgattgctg tctctcatgg tgaagggcgc gtggaagtgc gtgatgcggc
    33961 gcatctggcg gcactggaaa gcaaagggct ggtggcactg cgctatgtcg ataacttcgg
    34021 caaagtcact gaaacctacc cggctaaccc gaacggttca ccgaacggta ttacggcggt
    34081 tacgactgaa agtggtcgag tcaccattat gatgccgcac ccggaacgtg ttttccgtac
    34141 tgtcagcaac tcctggcatc cggaaaactg gggcgaggat ggcccatgga tgcgcatttt
    34201 ccgcaatgcg cgtaagcagt tggggtaagt catcagttca ttggttttgc agcccggatg
    34261 cggcatttac gccgcatccg gcattaagca ccaaccacta tcataagctc ccaactccgg
    34321 gagctttttt gtgtctgtaa atcacgacaa tgggtggttt accgtgtcgc tttctggcga
    34381 cacttaaatg attgatttta ttgtaatcta ataagtttat cttaagttgt ctctttttag
    34441 cgacacagtg gctgatttac attcaaatag cgaagaagcc ataaatccct caaagcaacg
    34501 tataatcagt acgttaccaa actattttct ttattggcac agttactgca taatagtaag
    34561 cagtggctca ttcaccgact tatgtcagcc ccttcgagac gtgctacata aaattcgaat
    34621 gacgcacaac aaggtgcctg ccgtccaact tctgatatca gcgtagctat atcaaccatc
    34681 gggcgaaacg tcgagttagg caccgcctta ttccataaca aagccgggta attcccggct
    34741 ttgttgtatc tgaacttccc ctcggttagc atcaggctat tcgcgtctga cgagagtaac
    34801 accttgaaac gctggcccgt ttttccccgc tcattacgac aactggtaat gctggcattt
    34861 ttgctaattc tactacccct gttggtgctg gcatggcaag cctggcaaag cctgaatgcg
    34921 cttagcgatc aggcggcgct ggttaaccgc actacgctta ttgatgcccg gcgtagtgaa
    34981 gcgatgacca acgcggcgct ggagatggag cgtagctacc gtcagtattg cgtgctggac
    35041 gaccctacgc ttgcgaaggt ttatcaaagc cagcgcaagc gttacagcga aatgctcgat
    35101 gcccacgcag gcgtactgcc agacgataaa ctttaccagg cattgcgtca ggatttgaac
    35161 aatctggccc aacttcagtg taacaacagt ggtcccgatg ctgccgccgc cgcgcgtctt
    35221 gaagcctttg ccagtgccaa taccgaaatg gtgcaggcca cgcgcacagt ggtgttctct
    35281 cgtgggcagc aacttcagcg tgaaatcgcc gaacgtgggc aatattttgg ttggcaatcg
    35341 ctggtgctat ttctggtgag tctggtgatg gtgctacttt tcacgcggat gattatcggg
    35401 ccggtgaaaa atatcgagcg catgatcaac cgtctggggg aagggcgttc tctgggcaat
    35461 agcatttcgt tctgtggacc gagcgagtta cgctcggttg ggcaacgtat tctttggtta
    35521 agtgagcgcc tgtcatggct ggaatcccaa cgccatcaat ttttaagaca tttatctcat
    35581 gaattaaaaa cgccactggc gagtatgcgc gagggcactg aattactggc tgatcaggtt
    35641 gtcgggccgc ttacgccaga gcaaaaagag gtggtgagca ttcttgatag cagcagccgc
    35701 aatttgcaaa aactgatcga acaactgctt gattacaacc gtaaacaggc ggacagtgcg
    35761 gtggaactgg agaatgttga attagcaccg ctggtggaga cagtggtttc tgctcatagc
    35821 ctgcccgcac gggctaaaat gatgcatacc gacgtcgatc tcaaagcaac agcttgcctg
    35881 gcggagccaa tgctgctgat gagcgtactg gataatcttt actccaatgc ggtgcactac
    35941 ggggctgaat ccggtaacat ttgccttcgc agcagtttac atggtgcgcg ggtttatatt
    36001 gatgtcatca atacaggcac gcccattccg caagaggaac gcgccatgat cttcgaaccc
    36061 ttttttcagg gaagccacca gcgaaaaggg gcggtgaagg gcagcggtct gggattaagc
    36121 attgccaggg attgtattcg ccgtatgcaa ggggaactgt atctggtcga cgagagcggg
    36181 caagacgttt gtttccgcat tgaattaccg tcgtcgaaaa acacgaaata atgctaacag
    36241 cctatcctat caggctattt tatttgtcag ttcggccccg ggtagtgctc gaaatcttca
    36301 cgtacgatat gtacgctccg gtttctccgc gctgtccatg tccagactgt ctgcaacaat
    36361 tacgcccact gggataggct ttaagtctgg tgaatatgcg acacattttt caacgattat
    36421 tgccccgacg gctgtggctg gcagggctgc catgtctggc attgctgggt tgcgtgcaga
    36481 atcacaataa gccagccatt gatacgccag cagaagaaaa aattccggtt tatcaactgg
    36541 ctgactatct ttctactgaa tgtagcgaca tctgggcgct gcaaggtaaa tcaaccgaaa
    36601 ccaatccgct ttactggctg cgggcgatgg attgtgctga tcgtttaatg cctgcgcagt
    36661 cgcgtcagca agctcgccaa tatgacgacg gcagctggca aaataccttt aaacaaggga
    36721 ttttgctcgc cgacgccaaa attacgccgt atgagcgccg ccagctggtc gcgcgtattg
    36781 aagcgttaag taccgagatc ccggcacagg ttcgcccact gtatcaactc tggcgcgacg
    36841 gtcaggcgct gcaactgcaa ctggcggaag agcgccaacg ttacagtaaa ctccagcagt
    36901 ccagtgacag cgagctggat acattgcgcc agcaacatca cgttctacag cagcaactgg
    36961 aactcaccac ccgcaagctg gaaaacctga ccgatattga acgccagctc tcgacccgca
    37021 aaccggctgg aaatttctcg ccggatacgc cgcacgagag tgaaaagccc gcgccatcca
    37081 cccatgaggt cactcctgat gagccataaa cctgcgcatt tattattggt cgatgacgat
    37141 ccgggattgc tgaaactgct tggcctgcgc ctgaccagcg aaggctacag tgtggtcacg
    37201 gcggaaagtg gcgctgaagg attacgggta ctgaatcgcg aaaaagtaga tttagttatc
    37261 agcgacctgc ggatggatga aatggacggt atgcagctat ttgctgaaat ccagaaagtg
    37321 cagccgggaa tgccagtaat tattcttact gcgcatggtt ctatccccga tgccgttgct
    37381 gcaacacagc agggcgtttt tagtttcctc accaagcctg tcgataaaga tgcgctatat
    37441 caggcaattg acgatgcgct ggagcaatcc gcgccagcca ccgatgaacg ctggcgcgag
    37501 gcaattgtca cccgcagccc gctgatgctg cgtttgctgg aacaggcgcg gctggtggcg
    37561 caatcagacg tcagcgtttt gattaacggt cagagcggca ccgggaaaga gattttcgcc
    37621 caggctatcc acaacgccag cccgcgcaac agcaaaccat ttattgctat taactgtggc
    37681 gcattgcccg agcaattgct ggagtcggag ctgtttggtc atgcgcgtgg cgcgtttact
    37741 ggcgctgtca gcaatcgcga aggtttattc caggcggcgg aaggcggaac gctatttctc
    37801 gatgaaattg gcgatatgcc tgcgccgtta caggtcaaac tgctgcgcgt gttgcaggag
    37861 cgtaaagtgc gcccgctggg cagtaaccgc gatattgata tcaatgtgcg gattatttct
    37921 gccactcacc gtgatctgtc aaaagcgatg gcgcgcgggg aattccgcga agatctctat
    37981 taccgcctca acgttgtcag cctgaaaatt ccggcactgg cggagcgcac agaagacatt
    38041 ccgctactgg caaatcacct gttgcgccag gcggcagagc gacataaacc gtttgtccgc
    38101 gcgttctcta ccgatgcgat gaaacgcctg atgaccgcga gctggccggg taatgtgcgc
    38161 cagttggtca acgtgattga acagtgcgtg gcgctgacct catctccggt gattagtgat
    38221 gcgctggtgg agcaggcgct ggagggtgaa aatacggcgc tgccaacttt tgttgaggca
    38281 cgtaatcatt ttgaactcaa ctatttgcgt aagctgctgc aaatcaccaa aggcaacgtc
    38341 acccacgcgg cgagaatggc ggggcgcaac cggacagaat tttataaact gctttcccga
    38401 cacgagctgg atgcaaacga tttcaaggaa tgaattggcg ttatgtgtta cgtttagcag
    38461 atcaaaagac aggcgacctt ttcaaggaat agcatgaaaa agattgatgc gattataaaa
    38521 cccttcaagc tggacgatgt ccgcgaagca ctggccgaag tcggtattac cggcatgacg
    38581 gtgaccgaag tgaaaggctt tggtcgccag aaaggccata ccgagctgta ccgcggcgcg
    38641 gagtatatgg tggattttct gccgaaagtg aaaattgaga ttgtcgtacc ggacgacatt
    38701 gtcgatacct gtgtcgatac cattattcgc acggcgcaaa ccggcaaaat cggtgacggt
    38761 aaaatcttcg tctttgacgt ggctcgggtc attcgcatcc gtaccggtga agaggacgac
    38821 gcggcaattt aaaccgcgtc aagggttgcc ggatgtttcc atccggcaac atcaaattac
    38881 agcaccttat gcgggccaaa gcattcgtaa tgaatgtttt cctgcttcac gcccagatcc
    38941 actaactgtt tcgcggtaaa ctgcatgaag ccaaccgggc cgcagagata gaactgcatt
    39001 gtcggatcgc tgaacgcacc ttccagtttg ctcaaatcca tcagaccttc gctatcaaac
    39061 tgacctttag cgcgatcggc ttcgctcggc tgacgatacc aggtgtgcgc ggtaaagcgc
    39121 ggcagtgact gccccagttc cttaacttca tcagcaaagg cgtgaacttc gccattttct
    39181 gccgcatgga accagttcac ttgtgctgtg tggcctgctt ttgccagcgt gtcgagcatt
    39241 gccagcattg gcgtttgacc aacaccggta gagattaacg tcactggtgt gtcatctgcg
    39301 acagccataa agaaatcacc tgccggagcg accagtttca cgacatcgcc aacattggcg
    39361 tgattgtgca accagttgga tacctgccca ccctcttcgc gtttcaccgc aatacgatag
    39421 cctttgccat ccggtttgcg agtcaaagag tactgacgaa tttcctgatg cgggaaacct
    39481 tccggcttca gccagacgcc gagatattgc cccggacggt attctgccac tgcgccaccg
    39541 tcgaccggct ccagttcgaa gctggtgata agcgcgctgc gcggtgtttt agccacaatg
    39601 cggaaatcgc gagtaccttc ccaaccaccg gctttgctgg cgttttcgtt atagatttcc
    39661 gcctcgcgat tgataaatac attagccagt acaccatagg ctttacccca cgcgtccagc
    39721 acttcctgcc ccgggctgaa catttcgtcc agcgttgcca acaggtgttc accgacgatg
    39781 ttgtactgtt ccggtttgat ctggaagctg gtgtgcttct gcgcgatttt ttctaccgct
    39841 ggcagcagcg caggcaggtt ttcaatatta ctggcgtagg cggcaatagc gttaaacagg
    39901 gcttcacgtt gatcgccatt acgctggtta ctcatgttaa aaatttcttt gagttctggg
    39961 ttatgagtaa acatacggtc gtagaaatgg gcggttaact ttggccccgt ttccaccagt
    40021 aaagggatgg tggcttttac tgtagcgatg gtttgagcgt caagcatatg gtcttccttt
    40081 tttgcatctt aattgatgta tctcaaatgc atcttataaa aaatagccct gcaatgtaaa
    40141 tggttctttg gtgtttttca gaaagaatgt gatgaagtga aaaatttgca tcacaaacct
    40201 gaaaagaaat ccgtttccgg ttgcaagctc tttattctcc aaagccttgc gtagcctgaa
    40261 ggtaatcgtt tgcgtaaatt cctttgtcaa gacctgttat cgcacaatga ttcggttata
    40321 ctgttcgccg ttgtccaaca ggaccgccta taaaggccaa aaattttatt gttagctgag
    40381 tcaggagatg cggatgttaa agcgtgaaat gaacattgcc gattatgatg ccgaactgtg
    40441 gcaggctatg gagcaggaaa aagtacgtca ggaagagcac atcgaactga tcgcctccga
    40501 aaactacacc agcccgcgcg taatgcaggc gcagggttct cagctgacca acaaatatgc
    40561 tgaaggttat ccgggcaaac gctactacgg cggttgcgag tatgttgata tcgttgaaca
    40621 actggcgatc gatcgtgcga aagaactgtt cggcgctgac tacgctaacg tccagccgca
    40681 ctccggctcc caggctaact ttgcggtcta caccgcgctg ctggaaccag gtgataccgt
    40741 tctgggtatg aacttggcgc atggcggtca cctgactcac ggttctccgg ttaacttctc
    40801 cggtaaactg tacaacatcg ttccttacgg tatcgatgct accggtcata tcgattacgc
    40861 cgatctggaa aaacaagcca aagaacacaa gccgaaaatg attatcggtg gtttctctgc
    40921 atattccggc gtggttgact gggcgaaaat gcgtgaaatc gctgacagca tcggtgctta
    40981 cctgttcgtt gatatggcgc acgttgcggg cctggttgct gctggcgtct acccgaaccc
    41041 ggttcctcat gctcacgttg ttactaccac cactcacaaa accctggcgg gtccgcgcgg
    41101 cggcctgatc ctggcgaaag gtggtagcga agagctgtac aaaaaactga actctgccgt
    41161 tttccctggt ggtcagggcg gtccgttgat gcacgtaatc gccggtaaag cggttgctct
    41221 gaaagaagcg atggagcctg agttcaaaac ttaccagcag caggtcgcga aaaacgctaa
    41281 agcgatggta gaagtgttcc tcgagcgcgg ctacaaagtg gtttccggcg gcactgataa
    41341 ccacctgttc ctggttgatc tggttgataa aaacctgacc ggtaaagaag cagacgccgc
    41401 tctgggccgt gctaacatca ccgtcaacaa aaacagcgta ccgaacgatc cgaagagccc
    41461 gtttgtgacc tccggtattc gtgtaggtac tccggcgatt acccgtcgcg gctttaaaga
    41521 agccgaagcg aaagaactgg ctggctggat gtgtgacgtg ctggacagca tcaatgatga
    41581 agccgttatc gagcgcatca aaggtaaagt tctcgacatc tgcgcacgtt acccggttta
    41641 cgcataagcg aaacggtgat ttgctgacaa tgtgctcgtt gttcatgttg gatgcggcat
    41701 gaacaccttc ttaggcctac aaaatcttgc aaattcaata tattgcaatc tccgtgtagg
    41761 cctgataagc gtagcgcatc aggcaatttt tcgtttatga tcatcaaggc ttccttcggg
    41821 aagcctttct acgttatcgc gccatcaaat ctgtcgtaac tgcgcctcaa catacaaata
    41881 gccaattccc agcacctgtt gtgcgcggct taattgcccg aagccaattt gcgtcgcttt
    41941 cacgctcggt ccttcgtcgc ggtcgaacgg gttaaatcct gtctggcgaa taatagtatt
    42001 aagccagttc tcaccaaagg cacaactgcg accgtacaac caaatctgat taatattgag
    42061 gatgttgagg aagttataca gactcaaacc aatggcattg gcagagcgat caacccagct
    42121 ggtgatccac agttctccac tctgccaggc agcgattaac tgcgcggttg tcagtttttc
    42181 aggatcaagc tgagtattaa ccggttgtga ttttagccat acccgcgcct gtttttttaa
    42241 tgcacttaac gaggcgacgg tttccaggca gccgtaacgt ccacagtcgc agacgacgcc
    42301 atccggatta acgatggtgt gaccaatctg tccgctacca tacaaactgc cgcgataaat
    42361 ttgctcgtta atcacgaacg acgagccaat gccgtaatca acgttgatca cgcagaaatc
    42421 ccgttcctgc gaattatttt gccatttctc cgccagcgcc agcatcacgc agtcattatc
    42481 gaccagcacc cgaatgccca gcttctcttc cagcagatac ttcacctcaa ctggcgttgt
    42541 ccacggcgct tgcggcatgg tttgcgatac gccggtcacc ggatcaacct gcccgtggat
    42601 tgccagcgcc agattgatgg tgcggtccgg ccacaattta cggtggcgat gccagcattt
    42661 ttcgatttcg gacagcagcg cctgcggcgt cggtacatca atctgtaaat actcaaattc
    42721 gcctttcgga cttaaacaag cattagcaac ctgacactca atactggtgg gcgtcacgtt
    42781 caggcacagc gtccagtcac cttccggcgc aatcagccat gtaccgctac tatgcccgcg
    42841 cgtctggctt tcatcgtcaa tattcaccac ccgtttttcg ctttccagtt cctgcaaaat
    42901 attactgact gccggaatcg aaatttgcgc cagccgggcc agcgttgatt tattggcgcg
    42961 cttttgccgg tacagcagtt ccagaatcac gcatttgtta tggtggcgaa tctgttgatt
    43021 gttaatgcag gctctcatct tcactaaacc ttgtttagtt aattgcgtga ttattccgcg
    43081 aaaacattgc cagattgttc aatacactgc cacaaatctt ttaattcagt atgtcttgtt
    43141 aatattgagg gcaccatgac tccagtaaaa gtgtggcaag agcgcgttga gatcccgacc
    43201 tatgaaaccg ggccgcagga tatacatccc atgttcctgg aaaatcgcgt ttatcaggga
    43261 tcgtccggcg cggtttatcc ctacggcgtg accgatacgc tgagcgagca gaaaaccctg
    43321 aaatcctagc aggcggtgtg gctggaaaac gactacatca aagtgatgat cctgccggaa
    43381 ctgggcggtc gggtgcatcg cgcatgggat aaagtgaaac agcgcgattt tgtctatcac
    43441 aatgaagtca ttaaacctgc gctggtgggg ctgctgggac cgtggatctc cggcgggatt
    43501 gagtttaact ggccgcaaca ccatcgcccg accaccttta tgcccgttga tttcaccctc
    43561 gaagcccatg aagacggtgc acagacggtg tgggtcggcg aaacggagcc gatgcatggt
    43621 ttacaggtga tgacaggttt caccctgcgc cctgaccggg cggcactgga aatcgccagc
    43681 cgcgtctata acggcaacgc cacgccgcgt catttcttgt ggtgggccaa cccggcagtg
    43741 aaagggggtg aagggcatca gagcgttttc ccaccggatg tgactgcggt gtttgatcac
    43801 ggcaaacggg ccgtctccgc tttccccatc gccacttact acaaagtgga ctactccgcc
    43861 ggagtggaca tttctcgcta taaaaatgtg cctgttccaa cctcatatat ggccgaaaaa
    43921 tcgcagtacg attttgtcgg cgcgtggtgt cacgatgaag atggcggttt gctgcacgtt
    43981 gccaaccacc atattgcgcc aggtaaaaaa cagtggagtt ggggacacag tgaatttggc
    44041 caggcgtggg ataagagtct gaccgacaat aacggcccgt atatcgaact gatgaccggt
    44101 atttttgccg ataaccagcc tgattttacc tggcttgatg cttacgaaga gaagcgtttc
    44161 gagcagtatt tcctgcctta tcattctctg ggcatggtgc aaaatgcctc ccgcgatgcg
    44221 gtgataaaac tccagcgtag tgagcggggg attgagtggg ggctgtatgc catctctccg
    44281 ttgaacggat accgtctggc gatccgcgaa atcggcaaat gcaacgcgtt gctcgatgat
    44341 gccgtggccc tgacaccagc gaccgccatc cagggcgtgt tgcacggtat caatcctgac
    44401 agactgacca ttgaactctc cgatgccgac ggcaatattg tactgagtta tcaggaacat
    44461 cagccgcagg agttgccgtt gccggacgtc gccaaagcgc cactggcagc acaagacatt
    44521 accagtacag atgaagcctg gtttatcggt cagcatctgg agcaatatca tcacgccagc
    44581 cgttcaccgt tcgattacta cctgcgcggc gtggcgctgg acccgctgga ttatcgctgt
    44641 aacctggcgc tggcgatgct ggaatataac cgcgcagatt tcccgcaagc ggtggcgtat
    44701 gccagtcagg cgctgaaacg cgcacatgcg ctgaacaaaa acccgcagtg tggacaagca
    44761 agtttgattc gcgccagtgc ttacgaacgt cagggacaat atcaacaagc cgaagaggat
    44821 ttctggcgtg cggtctggag cggcaatagt aaagcaggcg gctattatgg tctggcacga
    44881 ctggcggcgc gtaatgataa cttcgacgcg ggtctggatt tttgccaaca aagtcttcgt
    44941 gcctgcccaa ccaatcagga agtgctttgc ttgcataatc tgctgctggt gttaagtggt
    45001 cgtcaggaca acgcgcgttt gcagcgcgag aaactgctgc gtgattatcc gctgaacgcc
    45061 actctgtggt ggctgaactg gttcgatggt cgtagcgaat cagccctcgc gcagtggcgc
    45121 ggtctgtgtc agggacgcga cgttaacgct ctgatgaccg ccgggcaact gattaactgg
    45181 ggaatgccca ccctggcggc agagatgctg aacgcactgg actgccagcg cacgctgccg
    45241 ctttacctgc aagccagctt gctgccgaaa gccgaacgtg gcgaactggt cgcaaaagcc
    45301 attgatgtct tcccgcagtt tgtccgtttc ccgaatacgc tggaagaagt ggcggcgctg
    45361 gagagtattg aagagtgctg gtttgctcgc catttactgg cctgcttcta ctacaacaaa
    45421 cgtagctaca acaaagccat tgccttatgg caacgttgcg tagagatgtc gccggagttt
    45481 gccgacggct ggcgcgggtt agcgatccat gcgtggaata agaaacacga ttatgagctg
    45541 gccgcgcgtt atcttgataa tgcttatcag cttgcgccgc aggatgcacg tctgcttttc
    45601 gaacgagatt tgctggataa gttaagtgga gccacaccgg agaaacgact ggcgcgtctg
    45661 gaaaataatc tggaaattgc gctgaaacgc gacgacatga ccgcagaact gctcaatttg
    45721 tggcatctca cgggtcaggc agacaaagcg gcggacattc tcgccacgcg taaattccac
    45781 ccgtgggaag gcggggaagg gaaggtcacc agtcagttta tcctcaacca gttattacgc
    45841 gcctggcagc atcttgatgc cagagagccg cagcaggcca gcgaactgct tcatgccgcg
    45901 ctgcattatc cggagaattt aagcgaaggc cgtttaccgg ggcaaactga taacgacatc
    45961 tggttctggc aggcgatatg cgccaacgcg cagggcgatg aaactgaagc gatgcgttgt
    46021 ttacgtctgg cggcgaccgg cgatcgcacc attaacatcc acagttatta caacgatcag
    46081 ccggttgatt atctcttctg gcaaggaatg gcgctgcgac tgctgggtga acagcaaacc
    46141 gcacagcaac tgtttagtga aatgaaacag tgggcgcaag agatggcgaa aaccagtatt
    46201 gaggcggatt tctttgctgt ttcacaacct gacctgttat cgctgtatgg cgatttacaa
    46261 cagcagcata aagaaaaatg cctgatggtg gcgatgctgg cgtccgcggg actcggggag
    46321 gttgcgcaat atgagtctgc tcgcgctgaa ttgacggcga ttaatccggc ctggccgaaa
    46381 gcggcattat tcaccactgt gatgcctttt atttttaact acgttcacta aaccctatta
    46441 actgtaatat ttgagcggca cgcgctgccg ctcatcacaa aataattaga ataagccgtt
    46501 gttaataaaa accctacagg aggcactatg cctaaaaaaa tgagaacaac ccgtaatcta
    46561 ttattaatgg ctacgctatt aggtagcgca ctatttgcca gggctgcgga taaagaaatg
    46621 accattggtg caatatacct tgatacccag ggatattacg ctggagtgcg tcagggcgtt
    46681 caggatgcgg caaaagattc ttcagtacag gtacagttaa ttgaaactaa cgcccagggt
    46741 gatatttcga aagaaagtac ctttgttgat accctcgtgg cgcgtaatgt cgatgccatt
    46801 attttatcgg cagtgtctga aaatggcagt agccgtaccg ttcgtcgcgc cagtgaagcg
    46861 ggtattccgg tgatttgcta caacacctgt attaatcaaa agggtgtcga taaatatgtc
    46921 tcggcgtatc tggtcggcga tccactggaa tttggtaaaa aactgggtaa cgctgccgcc
    46981 gattatttta ttgccaataa aattgaccag ccgaaaattg ccgtcatcaa ttgcga
//
LOCUS       DADLSB010000014        46015 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.014, whole genome shotgun sequence.
ACCESSION   DADLSB010000014 DADLSB010000000
VERSION     DADLSB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.014"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            181..647
                     /locus_tag="JN331_000798"
                     /pseudo
     CDS             181..647
                     /locus_tag="JN331_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
     gene            complement(859..1023)
                     /locus_tag="JN331_000799"
     CDS             complement(859..1023)
                     /locus_tag="JN331_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259017.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1046..1747)
                     /locus_tag="JN331_000800"
     CDS             complement(1046..1747)
                     /locus_tag="JN331_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7259018.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1852..2748
                     /locus_tag="JN331_000801"
     CDS             1852..2748
                     /locus_tag="JN331_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7259019.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            complement(2955..3323)
                     /locus_tag="JN331_000802"
     CDS             complement(2955..3323)
                     /locus_tag="JN331_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7259020.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(3616..4602)
                     /locus_tag="JN331_000803"
     CDS             complement(3616..4602)
                     /locus_tag="JN331_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7259021.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(4672..5154)
                     /locus_tag="JN331_000804"
     CDS             complement(4672..5154)
                     /locus_tag="JN331_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7259022.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            complement(5250..5549)
                     /locus_tag="JN331_000805"
     CDS             complement(5250..5549)
                     /locus_tag="JN331_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259023.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(5539..5943)
                     /locus_tag="JN331_000806"
     CDS             complement(5539..5943)
                     /locus_tag="JN331_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259024.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(5946..6251)
                     /locus_tag="JN331_000807"
     CDS             complement(5946..6251)
                     /locus_tag="JN331_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7259025.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(6289..6657)
                     /locus_tag="JN331_000808"
     CDS             complement(6289..6657)
                     /locus_tag="JN331_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HAY7259026.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(6804..7187)
                     /gene="mzrA"
                     /locus_tag="JN331_000809"
     CDS             complement(6804..7187)
                     /gene="mzrA"
                     /locus_tag="JN331_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY7259027.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(7191..7853)
                     /locus_tag="JN331_000810"
     CDS             complement(7191..7853)
                     /locus_tag="JN331_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7259028.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEEQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(8198..8974)
                     /gene="exuR"
                     /locus_tag="JN331_000811"
     CDS             complement(8198..8974)
                     /gene="exuR"
                     /locus_tag="JN331_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY7259029.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            9270..9986
                     /locus_tag="JN331_000812"
     CDS             9270..9986
                     /locus_tag="JN331_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7259030.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAENQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            10016..10516
                     /locus_tag="JN331_000813"
     CDS             10016..10516
                     /locus_tag="JN331_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7259031.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            10608..11414
                     /locus_tag="JN331_000814"
     CDS             10608..11414
                     /locus_tag="JN331_000814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-fimbriae usher protein"
                     /protein_id="HAY7259032.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLN"
     gene            11442..11642
                     /locus_tag="JN331_000815"
     CDS             11442..11642
                     /locus_tag="JN331_000815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259033.1"
                     /translation="MVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVA
                     LKVYENNQLTRACAVYQNRRSD"
     gene            11611..13287
                     /locus_tag="JN331_000816"
     CDS             11611..13287
                     /locus_tag="JN331_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane fimbrial user protein"
                     /protein_id="HAY7259034.1"
                     /translation="MPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLH
                     PQYELYAGLANADDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSN
                     PGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTRTQNN
                     TDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNG
                     GDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSF
                     SHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSST
                     LAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDS
                     VLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNY
                     IGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVV
                     KKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            13740..14372
                     /locus_tag="JN331_000817"
     CDS             13740..14372
                     /locus_tag="JN331_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY7259035.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(14460..15758)
                     /locus_tag="JN331_000818"
     CDS             complement(14460..15758)
                     /locus_tag="JN331_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7259036.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            16241..17653
                     /gene="uxaC"
                     /locus_tag="JN331_000819"
     CDS             16241..17653
                     /gene="uxaC"
                     /locus_tag="JN331_000819"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY7259037.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRCPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            17668..19155
                     /locus_tag="JN331_000820"
     CDS             17668..19155
                     /locus_tag="JN331_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY7259038.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKRELGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            19238..19789
                     /locus_tag="JN331_000821"
     CDS             19238..19789
                     /locus_tag="JN331_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY7259039.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(19794..21038)
                     /gene="sstT"
                     /locus_tag="JN331_000822"
     CDS             complement(19794..21038)
                     /gene="sstT"
                     /locus_tag="JN331_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY7259040.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(21634..22599)
                     /gene="alx"
                     /locus_tag="JN331_000823"
     CDS             complement(21634..22599)
                     /gene="alx"
                     /locus_tag="JN331_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY7259041.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(22870..23886)
                     /locus_tag="JN331_000824"
     CDS             complement(22870..23886)
                     /locus_tag="JN331_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7259042.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHERGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLGSEIQGEAGALVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     YLPK"
     gene            complement(23947..24639)
                     /locus_tag="JN331_000825"
     CDS             complement(23947..24639)
                     /locus_tag="JN331_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY7259043.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGHKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(24716..25219)
                     /locus_tag="JN331_000826"
     CDS             complement(24716..25219)
                     /locus_tag="JN331_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7259044.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            25304..26440
                     /gene="rlmG"
                     /locus_tag="JN331_000827"
     CDS             25304..26440
                     /gene="rlmG"
                     /locus_tag="JN331_000827"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY7259045.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKVVKLGRRR"
     gene            26725..27039
                     /locus_tag="JN331_000828"
     CDS             26725..27039
                     /locus_tag="JN331_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HAY7259046.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKE
                     KK"
     gene            27036..27452
                     /gene="higA"
                     /locus_tag="JN331_000829"
     CDS             27036..27452
                     /gene="higA"
                     /locus_tag="JN331_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY7259047.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(27497..29515)
                     /locus_tag="JN331_000830"
     CDS             complement(27497..29515)
                     /locus_tag="JN331_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY7259048.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKITLQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(29741..32092)
                     /gene="ygjK"
                     /locus_tag="JN331_000831"
                     /pseudo
     CDS             complement(29741..32092)
                     /gene="ygjK"
                     /locus_tag="JN331_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(32109..33180)
                     /gene="ygjJ"
                     /locus_tag="JN331_000832"
                     /pseudo
     CDS             complement(32109..33180)
                     /gene="ygjJ"
                     /locus_tag="JN331_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(33314..34747)
                     /locus_tag="JN331_000833"
     CDS             complement(33314..34747)
                     /locus_tag="JN331_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7259049.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(34810..35259)
                     /locus_tag="JN331_000834"
     CDS             complement(34810..35259)
                     /locus_tag="JN331_000834"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY7259050.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(35256..38348)
                     /gene="ebgA"
                     /locus_tag="JN331_000835"
     CDS             complement(35256..38348)
                     /gene="ebgA"
                     /locus_tag="JN331_000835"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY7259051.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSH
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(38532..39515)
                     /gene="ebgR"
                     /locus_tag="JN331_000836"
     CDS             complement(38532..39515)
                     /gene="ebgR"
                     /locus_tag="JN331_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY7259052.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            39734..40065
                     /locus_tag="JN331_000837"
                     /pseudo
     CDS             39734..40065
                     /locus_tag="JN331_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(40107..41597)
                     /gene="ygjG"
                     /locus_tag="JN331_000838"
     CDS             complement(40107..41597)
                     /gene="ygjG"
                     /locus_tag="JN331_000838"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY7259053.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            41904..43424
                     /locus_tag="JN331_000839"
     CDS             41904..43424
                     /locus_tag="JN331_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7259054.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAILWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVAIMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQFGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(43578..44201)
                     /locus_tag="JN331_000840"
     CDS             complement(43578..44201)
                     /locus_tag="JN331_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY7259055.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            44478..45242
                     /gene="nfeF"
                     /locus_tag="JN331_000841"
     CDS             44478..45242
                     /gene="nfeF"
                     /locus_tag="JN331_000841"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HAY7259056.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEKYDPQRVRAAAYWHAK"
     gene            complement(45296..45371)
                     /locus_tag="JN331_000842"
     tRNA            complement(45296..45371)
                     /locus_tag="JN331_000842"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45336..45338),aa:Ile,seq:cat)
     gene            45496..46002
                     /gene="mug"
                     /locus_tag="JN331_000843"
     CDS             45496..46002
                     /gene="mug"
                     /locus_tag="JN331_000843"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY7259057.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT        11372 a        11626 c        11930 g        11087 t
ORIGIN      
        1 aagtgggggc tgcatattgg cgcgacgctg gaaaaacagt gcgagcgcgg tttgctggcg
       61 aaagatctct cttcatccat tgtgattcgt accagcgcgg catccgatgc gcgtatgggc
      121 ggcgctacgc ttccggctat gagtaactcc ggctcgggta accaggggat caccgcaaca
      181 atgcctgtgg tggttgtagc agaacacttc ggagcggatg atgaacgact ggcgcgtgcg
      241 ctgatgcttt ctcatttgag cgcaatttac atccataacc agttaccgcg tttgtctgca
      301 ctttgtgccg caacgaccgc agcaatgggg ccgccgccgg gatggcatgg ctggtggatg
      361 ggcgttatga aactatctcg atggcgatca gcagtatgat cggcgatgtc agcggcatga
      421 tttgcgatgg tgcgtcgaac agctgcgcga tgaaggtttc gaccagtgct tcggctgcgt
      481 ggaaagcggt gttaatggcg ctggatgata ccgccgtgac cggcaatgaa gggatcgtgg
      541 cgcatgatgt tgagcagtcg attgccaacc tgtgtgcgtt agcaagccat tcgatgcagc
      601 aaacggatcg gcagattatc gagattatgg cgagcaaggc cagataaacg ccaggttttg
      661 agtattgtgc cggatgcgat gctggcgcac cttatccggc ctacggggtg ctcaacttgt
      721 tgatgttgtt gtgttttgta cctgatgccg gatgcgatgc tggcgcatct tatccggcct
      781 acggggtgtt taatttgttg atgttgttgt gttttgtagg ccggataagg cgtttacgcc
      841 gcatccggca acctgtcttt acgcggtagc ttcatcctcg gctaaacgcg tttcagcatc
      901 cgcgacgata gcttccagct cgctcaacat ctcttcatag ccaaaatttt tggcggtgcg
      961 agcaggttct ttcgccacca cgggcttcgc tggctggcgc tttttggtcg attttttact
     1021 catcatcatc tccctgtaaa aacaactaga caggcaaatc tatcagcaaa gcgcgcagtg
     1081 gggaatcggc aaccagcgta atgttagcct cgtcacgaat aaacgcccca tcaccgcagg
     1141 ttagcgccgc tttctcttca tgatgcgtaa gcgcatgaaa tttcccgtga atcgattgca
     1201 aatacgcgcg tggcccatgc aactggaaat tcgcactttc gcctttgtcg agcacgatat
     1261 ggtgcagcca cacttgttgg cgtaattgca ggcttcccat cgcaccctct ggcgaggcga
     1321 ttaattgctg cttgtccata ttaagcgcca gcttttgaat cagcggattc tctcgctgcg
     1381 ggcaggtatc cagccaaagc tgcattcgcg ttaacggttt gtctttgctg agattgtgtt
     1441 cgctatagct gacacccggc tgggtagaga gcagcaacgc ctctccggcg ctggcctgaa
     1501 catgattgcc ttcgctgtcg cgatactctg cttctccatc cagaatcaca tttaaaatat
     1561 cgactttggg ataggttcgc ggctgaaagg cggcacctgg ggccagcact tcctggttaa
     1621 gcacacgcag ggaggcatag cctaacaatt tcgggtcgaa gtagtgtcca aaggaaaaag
     1681 tataccgggc ctgcaaccat ccgtagtctg cttgtccaca ctgcctggca gttcgggtag
     1741 taatcataat tcttgacctc tctttacttc cttttatggt aaggggctgg acgctacatt
     1801 gttagccaga tattctgccc ggtatgttca aatttcctga atgagaacga aatggccaaa
     1861 gaaagggcat taacgctgga agcactacgg gttatggatg cgatcgatcg ccggggcagt
     1921 tttgcggcgg cagcggatga gctgggacgc gtgccttccg cacttagcta caccatgcaa
     1981 aaactggaag aagagctgga tgtggtgctg tttgaccgct cgggccatcg caccaaattc
     2041 accaatgtcg ggaggatgtt gctggagcga gggcgcgttt tgctggaagc cgcagataaa
     2101 ctgactactg atgcggaagc tctcgcgcgc ggttgggaaa cgcatctcac cattgtgacc
     2161 gaagcgctgg taccgacacc tgcctttttc ccgttaatcg acaaactggc ggcaaaagcc
     2221 aatacccaac tggcaatcat cacagaagtg ctggcggggg cgtgggaacg gctggagcag
     2281 gggcgggcgg atatcgttat cgcgccggat atgcattttc gttcctcgtc ggagatcaac
     2341 tcgcgcaagc tctatacgtt aatgaacgtc tacgttgccg cgcctgatca cccgattcat
     2401 caggagccgg aaccgttatc tgaagtgacg cgtgtgaaat atcgtggaat tgcggtagcg
     2461 gataccgctc gtgagcgccc ggtgttgacc gtacagctgc tggacaaaca gccgcgctta
     2521 acggtgagca cgattgaaga taaacgtcag gcattactgg cggggcttgg cgtggcgacg
     2581 atgccgtatc cgatggtcga aaaagatatt gcggaagggc ggttgcgtgt cgtcagcccg
     2641 gaatcgacca gcgagatcga tattattatg gcctggcgtc gtgacagtat gggggaagcg
     2701 aaatcctggt gtctgcggga aattccaaag ttgttcgctg gaaaataatc tattaagcac
     2761 ctttcacaag gtgcttctga ctgctgacaa acgcaaaact gcctgatgcg ctacgcttat
     2821 caggcctacg cagtcactgc aatatattga attttggcaa tttgtaggct ggataaggcg
     2881 tttacgccgc atccggcata aacaaagcgc actttgtcaa caatttgaag caccttttac
     2941 aaggtgctta atcattattc ttgttccagt tttggatctg gtccaaagcg gttttcgcct
     3001 ggcgtacccg cctggcagtt aaagacgatg ataataagcc agccgataaa cgggattaag
     3061 aatagcagtg cccaccacgc cgagcggtcg gtgtcgtgca ggcggcggaa ctgaaccgcc
     3121 caccacggta aaaacaccag aataccgtag attgtcgtca ggatgccttc gccaccagcg
     3181 cgttgccagc ctaacatttt atccagcagc cccagcacga acgtaaagat gatgttgacc
     3241 agaataaaca tccagtactc tttgcgccgc gcacgacccc ggaaaccgac ataatttttt
     3301 agtactttca gataccagtc catttttgcc tcaattttgc tcaatcactt gtttttaaag
     3361 cagcaaatta agaatagacg tgaatgaaat ggcagtaaat attcgttcaa ggaattaata
     3421 aagtgccgtg aaggcgcgct aagactgatg gcaagcgcaa aatcgccaga tgcgctacgc
     3481 ttatctggcc tacgcggtca ttgtaacata ttgaatataa acgctttgta ggtttgataa
     3541 ggcgttcacg ccgcatccga catgaacaac atgcacttag ctaattatca gaggcacctc
     3601 gtaaggtgcc tttttttaac cgaagcgaac atctcgtcca tgcggttcat cgagatcctg
     3661 ccacggacca attgaaataa tccccgtagg gttgatggtc ttatggctgc ggaagtaatg
     3721 attacggatg tgatcgaaat tgacagtttc ggcgatcccc ggcatctggt agatatcgcg
     3781 caggaagcca tacagattca ggtaatcgct gatgcggtgc ttatcacact tgaagtgggt
     3841 cacatacact ggatcaaaac gcaccagcgt agtccacagg cgaatatcgg cttcggttag
     3901 ctggttgccg gtcaggtaac gatgctggcc taaaatctgc tccagtcgcg ccagcgattc
     3961 aaacactttc gccaccgcct cgtcgtaggc ttgctggctg gtggcaaaac cagctttata
     4021 cacgccgttg ttaacggtgt cataaatcca gccgttaagt tcgtcaattt tcgtttgcag
     4081 ggctggtggg tagtaatcac ccgcttttgc gcccagcgca tcaaacgcgg tattgaacat
     4141 gcggatgatt tctgctgatt cgttgctgac gatggtgtgg ttctttttgt cccacagcac
     4201 gggaacagta actcgtccgc tgtagtgtgg atcggcgtgg agataaagct gatacagaaa
     4261 ttcatgttga tagagcgtat ctccggttgc tcccggaaaa ctgtcatcaa aggtccagcc
     4321 gttttccagc atcagcgggt tcactacgga aacggaaata aacggttcca gtcctttgag
     4381 tttgcgcatg atcagcgtgc ggtgcgccca cgggcaggcg agtgaaacat agagatgata
     4441 acgatctttc tctgcgataa aaccgcctgt gccagtgggg ccaggtgcgc catcggcagt
     4501 gagccagtta cgaaatgcgg aagctgaacg ttgaaattta ccgccggtag atttggtatc
     4561 gtaccaggtg tcatgccaga cgccgtcaat cagttgaccc atttttctct cctccaatag
     4621 caaaaacgag gagcttcctc ctcgtttttt atcgttaagt atagcgcgtg cttaccactt
     4681 tttattcagc aggcggtcga tgctatacgc gcccggaccg gtaattgcca gcagcaggaa
     4741 tccgccagaa attgtcaggt ttttcatgaa catcagcgag ttgacgcctt cagcaaagtt
     4801 gctgtgaaat aaaaatgccg tcagcagcgt aaagcccgca gtaaacaggg ctgtggtgcg
     4861 agtcaggaaa ccgaacagga ttgccagacc accaccgaac tcaagcagaa tcaccagcgg
     4921 cagcataaaa cccgggacac ccattgcttc catatattgt tgggtaccag agtaagcggt
     4981 gatttttccc cagcctgcgg taataaacag aatcggcatt aaaatgtgcg ctaccagtac
     5041 accaacatct tctaattttt tcatcatact ctccactgcg ccactcaggc tgctgattgt
     5101 ttttttgtgc aagggcgcgg tattagcgtc gttgctgtcg atggagagaa tcataaacgt
     5161 ggtgaatgat gattgttagc aaggaaaact gtcaaaaata ttcaaaaaat ttgagggata
     5221 aggccggaat tgctccggcc agagggaagt taaccgcgaa gctgttgctg cttgagggtc
     5281 gttttaacca gacgccaggc gctccatacg ccaaaaccgc gtctggccca gcggaccagc
     5341 atattaggat ggcgaatcgt ccagatcgcc atcacgctac tgccaaccag cgcccaggag
     5401 cgcagactta gcagcatatt ccagcgacga tcgtaagcgc ctgttgcctc cagccattca
     5461 cgacgactgg cggaaagatc cagccgttgt tgctggatct ggctaagcag ttgcgcctta
     5521 cgtcgttcac gttcgacttt actgctcacg ggactcctcc tcgagcagtt gccgatcgtt
     5581 tgccagctca tggcgtgtat ggcgcagcaa ggtggattta cgcgatttac gtaacgtcca
     5641 gataccgcca atcagtgcca gtagcagcaa caccacggtg gtggcaatca tcgcattcag
     5701 gcgatattgc ggatcaaccg cccaaataat tagcaccatc aggctcataa gaccaaatgc
     5761 agcgaaaagc atcgtcaggc ccagcatcag taaaagttga aagagattag ctttttcctc
     5821 ttccagctcc accaccgcca gccgcagacg tgtttccacc atttcaacca tgatggaaac
     5881 aattcgctgc ccgatgccca gaacgctttt accgggccct tgcgcgtgat gagtgtccgc
     5941 cataattaac gacgcgacag cagaacgccg agcactacac cgattgcagc gccaattccc
     6001 acgccggtcc acggattttc acgcacatac tcatcagcac gcgccgccgc gacacgggtt
     6061 tgtttggcga tggcatcacc ggtttcaccc aggcgataac ggctctgttt cagtgcctgc
     6121 tccgctttgc tacgaatctt actcaactct tctttcgact tctcgccaga tgagctaagc
     6181 acctcttcca gcgtatcgga aagggatttc aactcagcac gcagatgttc cgtagtgtgt
     6241 tctttcgaca tagttttctc caggtgagta agtagtgact attgtgagtt agtagtcgcg
     6301 cgcttccagc tttttcagct cttcctgcgc ttctgccagt ttccgttcgc gtttggcaat
     6361 cttatcggca tcgccttttt gcttcgcctc ggctaaatct tgctggcgtt ccgccacctc
     6421 atctttctgc tttgcgattt tcttgtgatg atcggcacgc agctggctat ctgaacagtt
     6481 ggcccggact tcactcaggg ctttattcag accgtcaata cgattctggt tttggtgttt
     6541 ttcggcatag ctgatctcct taaggatatt ttgctccttt tcctgacaca gggtagtggc
     6601 ataactaccg gcactaagag caaagagaga aacagctaaa gcgatgcggt atttcattct
     6661 tgaaccttcc attgtgattc ggacctatta caaaggaacg attgtccaga caacgaagcg
     6721 gtagtgttaa ccaccgcctc attaatcata gacagttacg gtgaaatcac caaagtgagt
     6781 gatatgtttc ggataacagg aagttatccg aagcgatgag agttatcccg taaccgggtc
     6841 agccactgca tagcctgact gttattgtcc tgctgcgcaa tgatgtagcc atggggcaat
     6901 gttctgtcga ggaccgcttt tgcggcggcg ctctggtcgc tggagtcaaa tgtaattaac
     6961 agagtgtcgt ttttgggggt gatacttttg aaaggaatgc catgagcgtc aaggtgatgc
     7021 cagatcgaaa aaccgtctgg catcgttgtg ccttgatgaa cggcacgaat cgccagcgta
     7081 gactcttgct ggcgaaccgc ggaccaggcc agcaacagcg tgccgaccag aagtaaaaca
     7141 gtgccggacc aggctagctg gcgaagcgac atgcgaggta tttgcatccc ttacccccga
     7201 tttccatatt tctttttcca taacacgacc agagaacctg ctaggccaaa caccagcagc
     7261 accaccggga gcagcatcag gcatgacatc agctgttcct cgtactttaa aaataccggc
     7321 gttttgccga gcatgtaacc cagagttgtc aggatcaata cccacagcag accgctcatc
     7381 cagttgaaga actgaaagcg cgcgttattc agccctgata acccggcaat cgtcggcagc
     7441 agtgttctga caaatgcaat aaagcgacca attaacagcg ccgataaacc gtgtttatga
     7501 aaaagatggt gtgcgcgttg atgataatgc gcgggtaaat gagatagcca gttttgtacg
     7561 gtgcgggtat tgcccagcca tcgcccctga atatagctga cccagcaacc gaggctggcg
     7621 gcaacggtca gcagcagaat cgtttgcggg taacccatcg cgcctttcgc aatcaacacg
     7681 ccgaccaata ccagtaaact gtcgcccggt aaaaaggccg ccggaagcaa gccattttca
     7741 aggaacaaaa ttacaaacaa gacaaaatac aacatgccaa tcatcgatgg attggccagg
     7801 gtttcaaaat cctgcgccca cagggcttgc agcaattggg tcaaaagttc cattcattat
     7861 tcctggtgaa tcggttaacg ccacgctggt tttaaaagat gcactaccgc aatgttattg
     7921 ctgttttggt atgttctgcg ggtttgatac atcaacacag cctgaactat tccctgttaa
     7981 aacgtgagat aagaaagcat tcaaaagcat aaattcaaat tgaatctaat tgtaacaaag
     8041 gtcaacgttg ttcgttatca aaattacatg tccgtggagt ctgattttgc tgattgcagg
     8101 ggagggtggc gagaggattt acacaggctg acactttata cttttcgctt accgacgaga
     8161 caaacaggcg cattgcagcg cctgtcggac aggaatttca tttgctgccg ctggtggccg
     8221 tatcgagatg cactaccggg ttttcggcga acagatagcg gtcggcattg aactcgaaat
     8281 cgtcgctggt ttcgttaaat aacatgattt tggtgttttc cagatgctgc cacattgcca
     8341 gcttagcagc atgaggatct ttgcgaatta acgccttgag aatttgatcg tggtcatcac
     8401 accagttatc aacggtacgg gaatcaatgt gttcgtgtag ttttttccag tacgggttat
     8461 ggctacgctg ggtccacatt ttttcaacga tggccgccag ggcggagttc tgcgtcgcca
     8521 gggctacctg aatgtggaac tgtaaatccc actcggaatc acggaagcat tgttcgccgc
     8581 gcgcctgttc ctgaatggcc atcagtttca tgatgtcttg tttcgttacc tgagttgccg
     8641 cgaactcggc aatattactt tcgatgagct ggcgagcctg aagcaactca aacggaccgt
     8701 aattggcgaa ttccatatta ttgtcagccg cctgctgatg gcgcggctgg ttggaaacca
     8761 catgaatacc tgaaccttta cgcacctcca catagccttc aacttccagc atgatgatgg
     8821 cttcacgaac taccgttcgg ctgacgttct tttcatcggc aataaagcgt tcggcaggca
     8881 gtttatcacc caccagatag acgccctgtt caatgcgctc tttcaggtca gcagcaagtt
     8941 gttgatacaa acggcgtggt tcagtgattt ccatatgcgt ctcccggagt ggaatgcgga
     9001 cgaagttatc ataccacttt attcatgggt atgccattgg tcatatataa atttgatgat
     9061 tgaattaatg tattaaatta atattgtgaa tatttataaa ttcctaaaaa ataagtatcg
     9121 tcattgtgaa agctactaaa tttcagtaag ttaattccca gcctttaaat ttaatgttaa
     9181 ctaaaaatgc ttttttatat tgacttacaa taaatctaca tctctaatac accaactttt
     9241 ttgaaacgat attgtaagat caaaattata tggtggcgaa aattaatctc ttattacggc
     9301 ttacgccgct tttattcgtg ttcgctcctt ttttggcaca gtcaaatatg actgtttatc
     9361 ctatggctgt ttccattaat agtcagggtg aaggcaacgt tcgcgtaata tctaaaagta
     9421 atgaagttca gtacatcaag gcgacagtat tccgtatcga taatccatcg acgcctcagg
     9481 aaaatgaagt tgagattaaa tccggtgatg caaatcatct ggttgttatg ccacctaaat
     9541 tcgctttacc agccggtagt agcaaaaccg tacgttttgt tgcgatggaa ccagagcaaa
     9601 aagagaaaaa ttatcgcgtt aaatttgaag cggttcccag tattgatgac gttgccacag
     9661 ataaaaaaga tctctctatg cagttaacag ttaacttaat ttggggaatc gttgttagtg
     9721 ttccacctca gcaacctata gctaagttag aagtaaattc tgcccaaaaa ttaattaatg
     9781 cagagaatca acgcttaaag cttttaacga tcgcttattg taaaaataat agcaaagaaa
     9841 actgtaagat acagaccgta aataaaaaca tcttccctgg tcaggaaaga aatcttgaaa
     9901 gcatatctgg ctacgacaaa atcgttgtca aatataataa ctggatcacc aaagataatg
     9961 gcgagtttga actggcggtc cattaattat cattgtaata aacgaaggaa tagtaatgaa
    10021 aaaggtattt gcaaaatctc ttctggtcgc agcgatgttt tctgttgcag gctccgcatt
    10081 ggcagtacaa aaggatatta ctgtaacggc caacgttgat gccgctctgg atatgacaca
    10141 gaccgataac accgcgttgc cgaaagcagt agaaatgcaa tatctgccgg ggcagggtct
    10201 gcaatcttac cagttgatga ccaaaatctg gtctaacgac gttaccaaag atgtaaaaat
    10261 gcagttagtg tccccagcgc aactggttca aagcctggat gctagcaaga ttgttccgct
    10321 gacagtaacc tggggtggtg aagaaattaa agctgatgcg gcaaccactt ttaccgcaac
    10381 caaaatcttt gcttccgatg cgttaactaa cggttctctg gctaaaaacc tgatgtttgc
    10441 tcaaaccacc aaaggtgttc tggaaacagg catctaccgt ggtgtagtaa gcatttatct
    10501 gtctcaggat atttaatccg gataacagaa ttaacacgta acaaagggag gggtagccct
    10561 cccttatttt aactgtttta gtatttgtta tggatttcag gattgccatg gataaaaaat
    10621 tactggctct tttgatcctg gcgagtctca gcccggcaga ggcgacatta accaaaattc
    10681 ccgcagggtt tgaggttatt gctcagggac agcaggagta tatcgaggtt tatttttcag
    10741 ggaaaagtct cggtaaatat tatgcaatgg ttaatcttga taccgtaaca tttcttgatc
    10801 cagcaagtct atataacaag ctggaactgg atgtagacga tcagaaaatc gcgcatatag
    10861 tgaaagaaaa attatcgcag ccgctagctc gccacggtga attggcttgc ggttatgtac
    10921 gtactgactc agggtgtggt tttctgaata ccgatacgct ggaaataatc tataatgatg
    10981 aagaaagttc ggcaacgttg tttattaatc cgcaatggaa ttcagctttc gatgcgaagt
    11041 cattatattt aaatccagac aaaaatactg ttaacgcttt tatacatcag caagacatca
    11101 atgttctggc acaggatgat taccaatcgt tgtctattca gggaaacggt gcgctgggaa
    11161 taacagaaaa tagctatatt ggtgcacact ggaatttcaa cggttatgat gcagatgatg
    11221 tcagtgacag taatactgac gtcagcgatc tctattatcg ttatgatttt ttacgtcgtt
    11281 attatgtgca ggcgggtcgc atggacaacc gcacactatt taatgcacaa ggcgggaact
    11341 ttacctttaa ctttctgcca ctcggtgcaa tcgatgggat gcgtatcggg tcgacactga
    11401 gctatttaaa ctaggcgcaa agccagcaag gaaccccagt aatggttctg ctttcgcgca
    11461 attctcgtgt tgacgcttat cgtaatgagc aacttttggg atcgttttat ctcaatagtg
    11521 gttcgcaatt tattgatacc agttcctttc cgccaggcag ctacagcgtg gcattaaaag
    11581 tctacgaaaa taaccaactc acccgagctt gtgccgttta ccaaaaccgg cggtctgact
    11641 gacggaaatg cgcaatggtt cttacaggca ggtaaaacta catcacaggt ttctgatgat
    11701 gaaagttcag cttatcagct aggagtacgc ctgccattac atccgcaata tgagctctac
    11761 gcagggctgg cgaatgccga tgatgtgagt gctttcgagt taggtaataa ctggacggca
    11821 gatttaggcg gggtggggaa tcttgcaatc agcgccagcg tgttccgtaa cgatgacggc
    11881 ggcaaaggtg atatgcaaca ggccaactgg agtaatccgg gatggccgac gttgggcttt
    11941 tatcggacca actctgacgg cgatgcttgt acaaccgaca gcagagagag ctataacgcc
    12001 ttaagctgtt atgaaagtat ttccgcgaca gtttcactga attttgtcgg ctggaatatg
    12061 atgctgggtt atacccgcac acaaaataac actgatgata gtttgcgttg ggataaacag
    12121 cagagctttg aaaataacta tcttcgccag acaacagcgc aaagtatctc tgaaactgta
    12181 caacttagcg cttcccgcgc tattgtgatg cgtgactgga ttttgagtac gtcagtcggt
    12241 gttttccatc gtaatgacaa cggtggcgat aacgacgaca acggcttgta cttatcgttt
    12301 tcgttatctg acacgccaac gatggatagc aataacaaca gccattcaac caatgtgtct
    12361 acggattatc gttatagcga acaggatggc gatcaaacgt catggcagtt atcgcatacc
    12421 ttttataacg attcattcag ccataaagaa cttggcgtaa ccgtcggagg cctgaacacc
    12481 gataccataa acagcgcggt taacgggcgt tgggatgggc aatacggaaa tgtctacgct
    12541 accgtatctg atagttatga ccgtaagaat catgatcatc tttcggcctt tacgggcact
    12601 tatagctcta cgctggctgt cagtcgctat ggcgttaatt tgggagccag tggtacagac
    12661 gatttgctgg gtgcggtact ggtggatgtg aaaggcttct ctgaacagga tgaagagagt
    12721 caggatctgc aactcgaagc gcgggtggca ggcagccgaa ctttgcagct tggtcaaagt
    12781 gacagtgtgt tgttccctta tcctggattt cagtctggtt ttgttgaggt taacgacagt
    12841 agccagggca atcagcaggg gacaacaaac atcattaacg gtgcagggaa tcgtgaatta
    12901 atgttgttgc ctggcaagct gcgctatcgc gaagtgtctg ccagctttaa ctacaactat
    12961 atcggtcgtt tattactgcc ggcagcggtg aaaaaattcc cgattgtggg gctgaacagc
    13021 gccatgttac tggtagctga agatggcgga tttacacttg aaattaacgg cagcgaaaaa
    13081 gagctctatc tgctttccgg gcagcaattc cttaagtgtc cactgagtgt tgtaaagaaa
    13141 cgcgccagca ttcgttacag cggagatgtt acttgtagtg tggtgactta ttcacaatta
    13201 ccggagtcga ttcaggttca ggcacagttg aaacagccta aattacgtgg aaacgttcag
    13261 acggcgcaaa gggaggttgc accatgagaa atcgattgat tgcggcgata ttgggcttgt
    13321 ttggcacgct cactggcgtt caggcagctc ctgacgtgac cagtgaaatt acgtatgatt
    13381 tggcatctgg tagagcggat tattacttct ggaaggatga agcgtcggca ggaaataatg
    13441 gatatatgtg gtatgaatgt tcgtatcctg acctccaaca aacctgtaca gctaatggaa
    13501 atatatcgac agtacaaatc tatttaactg aacaacgcag tgggatgcgt tggccgataa
    13561 aactcaaagg atttaaaaca gccattgtaa gtagcgatga agcgccgcta ggatgcaagg
    13621 ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt acagaagatg
    13681 gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag cagacagaga
    13741 tgaagaattt gccgattggt ggcgtctgga aggggaaagt taaattacat tcgaacagcc
    13801 cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac catattgacg
    13861 tgttcttccc ggagttcgcc cacgccacgc caagggtgca gttagacttg catccaacag
    13921 gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg tgcctgtacg
    13981 atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa gggcgaccag
    14041 ctgcagggcg cagagacggt tacttctcta tctatcgtca gggagggacc accaccgacg
    14101 agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt gggcaaattg
    14161 atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaacgt gtgcgtccgg
    14221 tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg actctggcag
    14281 tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa ttgtcagtaa
    14341 tctttacgcc ttccttgcca accatcaatt aaccctgcaa accggacaaa cgcttttttc
    14401 tatataaacg acgaagccgc ctgataaggc ggcttcgtcg ttataactta cgttaaacgt
    14461 taatgttgcg gtgcgggatc gttatgtgtt tcctgcgcca cctcaatcgc cggtttgttc
    14521 tgcaatacgg tccagataac cagcgcaccc agcaggtcaa atactgccag aactgcgaac
    14581 agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgcaaa cagcgtactt
    14641 gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac
    14701 acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgggcaaa accaccgata
    14761 cacagcagca taattgcgac atacgggttg gtgaacagac cgatcatacc cgggccaatc
    14821 atcagcacag cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac
    14881 caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc agcaaacagc
    14941 atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac tttaaacatg
    15001 aacagcggga tccacgcgtt aaaagtaccc caggccggtt ctgccaggaa acgcggcagt
    15061 gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt cttcgccgtg
    15121 ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc atcggtcaga
    15181 tgcttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca gataaagctc
    15241 aatgcaccgg agatgataaa tgccatctgc cagctgtgca tcacgattgc ccataccacc
    15301 agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtagcc tactgcgatg
    15361 gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg gatcatcgct
    15421 gcttccgcgg caccgaccgc accacgagca acagccaggc caccccagct acctgccagc
    15481 gcggttgcac cacagaacac ggcccacagc acagcaaaca ttgcataacc gattttcgta
    15541 cccagcacat ccagtacata acctgctacc ggttgcatga ccgtataagc agcagaatag
    15601 gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat cagagttggc
    15661 gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccgag cgtcaccagt
    15721 gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat cgttttgtta
    15781 tatccgcagg aagcgcgacc atcatcccgc cagaggctga agatgttagt tacgaccggg
    15841 taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg ctaattcggc
    15901 ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt tgcattccgt
    15961 cgacttatga tttgcgacgg cagaaagata acttgtcata caaccttaaa aggtgagagc
    16021 catcacaaat gtgcgaatat ttgtagggac attacctgac gacagcgagg ccagtactgg
    16081 cgcggcctgc agcgagattt accactttga gagtaatttt tttaactacg tttattgatc
    16141 taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt cagcatcgca
    16201 ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt ttatgactga
    16261 agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg caaaagacca
    16321 gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag actatcgttt
    16381 taaaaacctg tatgacatct ggttgaaggg cgatcactac aaatggcgcg ctatgcgtac
    16441 caacggtgtg gctgagcgtc tgtgtactgg tgatgcgtct gaccgtgaaa aatttgacgc
    16501 ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga cgcacctcga
    16561 actgcgttgt ccgttcggta tcactggcaa attgctttct ccgtcaactg ccgatgaaat
    16621 ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg gcatcatgca
    16681 gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc tggagcatca
    16741 cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct ggcgtccgga
    16801 caaagccttc aatatcgaac aggcgacctt taacgactac atggcgaagc tgggcgaagt
    16861 ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca aacgtctgga
    16921 tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg tgatgtttgc
    16981 tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg gcgaaaccct
    17041 gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg gtgccgaata
    17101 tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata acaacctgcg
    17161 tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc gtccgatggc
    17221 ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctactgc cgaaaaccat
    17281 tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg gtaacttcca
    17341 gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta acgatcagaa
    17401 agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga gccgctttgt
    17461 cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat acttccgccg
    17521 cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg cggacatcaa
    17581 cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact acttcgccat
    17641 tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc gctggataac
    17701 gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga taaccagact
    17761 gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga tatcgcaaaa
    17821 ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga tattgcggcg
    17881 ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga tcagtatcgc
    17941 tatcaacctg attttcagga tctgcctgcg caagcggcag atcctgaagt gcagatctat
    18001 cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc aaccgtgggc
    18061 tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac caacaacgcc
    18121 gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca actgggcgac
    18181 gatcacatca acacccgcac catgctgcaa aacatggtgc gccacccgaa cgcgggcgca
    18241 gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg tgaaacgttg
    18301 ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga tgatgagatc
    18361 gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga taaacgcgag
    18421 ctaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga cggtctttct
    18481 ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc taacggcggt
    18541 actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct gatggaccat
    18601 tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt caaacagtac
    18661 tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa agcgggcggt
    18721 atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc cagtgtcgtg
    18781 gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccag ggctgaactt gttaagtgcg
    18841 ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca tatggtgctg
    18901 ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa aatcgccacc
    18961 aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg tcagctgatc
    19021 cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt tgagtttgcc
    19081 aacggtaagc aaacctgcaa cgagcgtaac gacttccgcg aactggcgat cttcaaaagc
    19141 ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc ataatgtgaa
    19201 gcgccgtttg cttttccata cctgctaagg atttatcatg accgcgtatt ggctggccca
    19261 gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc gtgacgaacg
    19321 tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt tttttcttct
    19381 gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat tgattacctt
    19441 acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg gaattggcct
    19501 cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg tcagtacctg
    19561 ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt caacgtgttg
    19621 ctgggtgatt cacaacttct gggcggggtc gataggcggc acgatgattg agggtagttt
    19681 tctgcttatg aatggcctga atatcattcg tttctggcgg atgcagaaaa ggggaattga
    19741 tccgtttaaa gtagagaaaa cccctcccgc catagacgaa aggggttaaa caattaatta
    19801 cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc agtgaacagc
    19861 acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc gataataaag
    19921 ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt acaggccagt
    19981 gggatcagca gcaaagaacc ccccgccacg ccggatgcgc cacaggcaca cagagaagcc
    20041 actacgctca acagcagcgc tgtgggcaga tcgaccggaa tacccagcgt attaaccgca
    20101 gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt ggctcccagc
    20161 ggaatagaaa ctgaataggt atcgcgatcc agattcagct tttcacacag cgccatattc
    20221 accggaatgt tcgctgcaga gctgcgggtg aagaaggcat acacaccgct ttcgcgcagg
    20281 cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag caatgggtta
    20341 accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg cgcgtagccc
    20401 cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagcccaaa aataccgatc
    20461 ggtgcgaagc gaatgaccag tttcaccata aaggtaacgg cattcgacat atcattgacc
    20521 aggtttttgg tggtctcgtt accgtgacgc agtgcgaagc cgaggccgat cgcccacacc
    20581 agaatcccga tgtagttacc tttcagcagg gcgtcgatgg ggttggaaac catgctcatt
    20641 accagtccgc gcatcacttc gacaatgcct gacggcggcg aaatatcacc cgcgctactg
    20701 gacaagtgca gggtagaagg gaaggcaaag ctgaagacta ctgcggccag agctgcagag
    20761 aaggtgccca gcagatagag gaacaaaata ggacggatat tggttttctg cccgtgctgg
    20821 tggttagcaa tagatgccat taccagcatc aacaccagga tgggggcaac agctttcagt
    20881 gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt tgagatccat
    20941 gccagaagaa tccccagaac aaggccgacc aggatttgtt ttactaggct gccatgagcc
    21001 agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt tcattgtgtt
    21061 gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa agtgtttgca
    21121 aggtcgagta taaggaaagt tacagtttcg ggaagcgaaa aatgctggat tttaaagaga
    21181 ggtcatgttt tgcaacttaa tggttgcatt tatttaacat aaacagaggg aaggactggc
    21241 tactaaggct gattagataa catatgggca aaactatttg gaatatgaga acggttgcgc
    21301 tcctttaacg tgttatgcca tataaagtca ctggcgcagg gagcgcgcag ggggcggcca
    21361 atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct gcgcggttcc
    21421 ctcagcctat cccttgcggc taccgggtcg ggcgcaaggt aacgtccctg taagatgcgc
    21481 cctcgccccg gcctccaggg aacgcttcgg cgattttgac gccaccaaca cccgtgcggt
    21541 ctttttgtaa agagctataa gccgattaca taattaatag ctcacctttg tctggaatga
    21601 gttatctcta ggaatggctt aatatacagc gaatcattct ccccgctgct tatcatgccg
    21661 ataattcacc caggcgttga taataaacgt catcaccaga atgccaaaca ccacgcccag
    21721 cgagacggcg attggaatat ggtagaagtc gacaatcagc atcttgatac cgataaacac
    21781 cagaatcacc gccaggccat atttgagcat cgagaaacgc tctgctacgc ccgccagcag
    21841 gaaatacatc gcacgcaggc cgaggattgc aaacaggttt gaggtcagca caatgaacgg
    21901 gtcagtggtc acggcgaaga tagccggaat gctatccacg gcaaaaatca cgtcgctcaa
    21961 ttccaccaga atcagtacca gcatcagcgg tgtggcgtac agcaatccat tcttacgcac
    22021 aaagaaatgc tcgttgtcga tggtgtcggt catgcgcaaa tgaccgcgta gccagcgcac
    22081 cagcggcttg tcgccaatgc ctgattcatc ttcatgggcg agcgccatct tcacgccggt
    22141 aaacagcagg aaggcaccga agatatacag gatccagtcg aactgcgaaa tcagccagct
    22201 gcctgtgaag atcatgatgg tacgcagaac aatcgccccg agcacgccat acaccagcac
    22261 gcgacgttgt aatgccgccg gaacggagaa atagctgaac aacatcagcc agacaaagac
    22321 gttatcgacc gccagcgatt tctcaatcag ataacccgtg agaaaggcca gggcctgtgg
    22381 atcggcaacg gcgcgacctt cggtttgcac cagataccac cagaaagcgg cgttaaacag
    22441 taacgacagc gtcacccaga ccagcgacca ggccgcagcc tgtttcatgg tcatggcatg
    22501 tgccccacga cgcccctgca acaacaggtc gatagccagc ataatggcga caacaacagc
    22561 gaatccgccc catagcaacg gcgtgccgac agtattcata gaagttcctt acacataaaa
    22621 aaacggccaa cgtcggaaga cgtcagccgc tgctttttat gcatagacct cgccttccgg
    22681 caaggtctca cttacaacaa aaaaggaata tgtctgcgta ttcctttcgg gttgcccggt
    22741 gaccggatgt ggtttttcac acatcgtaat gacgatcgac cggcaatgaa gttactcccc
    22801 tttgcaggta acaaagtatg acagaccatt cagtcagtca atggtctgtt acataccttt
    22861 tacactgttt tactttggca agtatagttt tacgctatct gccggaaaaa tcaccccagt
    22921 ctggcggcgg atctcggtca gcagtttggc tgtgatgcga ctgaccgcca gccctggatg
    22981 atccaccaga tgctcatcca ccagggtagc gaacagctct gcttcgtaga gcatggtatt
    23041 aatatgctgc ggctgggtga gatcctgcat ctggctgcca cgcggcacaa agcagacttt
    23101 ctggcattca gacagttttt caatcaccag cgctcccgct tcgccctgaa tctcgcttcc
    23161 caggacagaa tcactgacct ttgagtgctg caaggtgacg ctgaaatcac cgtaatccat
    23221 caccaccacg ccgtgggcgt caacgccgct tgccagcaaa ctcgccgttg cctgcacgct
    23281 tttcggctca ccaaataacg ccaccgccga cgccaggcag taaaagccga tatccataat
    23341 tgaaccgtta gagaatgccg gattaaaggt gttgggattc tcaccgtcca ggtaacgttg
    23401 ataccgcgag gaatactggc aatagttgaa aaagacttta cgcagtttgc ctgctttcgg
    23461 cagcgcctgg cgcaacaaat gaaagttcgg caggcaggcg gttttaaatg cctcaaacag
    23521 caccacctga ttttcgcgcg cacaggcaat ggcggcatcc acttccgcca gattcgacgc
    23581 cagcggtttt tcgcaaatca catgaatttt atggctaagg aaaagttgtg tctgggaaaa
    23641 gtgcagggaa ttcgggctgg caatatacac cgcgtcaatg gcatcgcttt ccgccatcgc
    23701 ttccagcgac gtaaacagat gctcgacaga aaaatcattg gcgaagtgct gagcctgctc
    23761 aaggctgcgg gaatatacgg cggttaactt gtatttaccg cgctcatggg cggcctcgac
    23821 gaactggcga gtgatccagt tcgtaccaat cacagcgaaa cgtatcataa cgcgtggata
    23881 ctccataaag ggattctgtc gtcaatgtag cacgcctttt tcatcttcga cgtgcgcaga
    23941 tacgcattat tctttcgccg gacagccatg ctcactaaac ggcccaatca tgaccgatgg
    24001 cccgagatat ttcggctggc gatggaggat ttttgcgtca atcaccgcgc tgacacgagc
    24061 gagtttctcc cgcagttgag tataaaaacc gtgcttcata ttgaggtctg gcgcgttaaa
    24121 cccgatagcg tcgatgccgt actgttttgc cagccagatg gcgcgttggt tatgaaattc
    24181 ttgcgagata atggtgatat ggttttcccc aaacaccttt ttggcacgca ctaccgaatc
    24241 cagcgttgag aatccggcat agtcgcaaaa gatcactttc gccggtacgc ctttagcgat
    24301 caaagcctgc tgcatacctg atgcttcatc gtaattttta tgtccgttat cgccactcac
    24361 cagtagccat ttcacttttc cggcgtggta caacgctgct gcggtatcaa tacgtcgggt
    24421 aaagtagcga ttacccggcc tcgcccccag caataaaccc acgttacgcg ccgggacggc
    24481 gttgacatcg ccccaggtca gctgcttgct ggcgttgacc ataacgcgat cggcgataaa
    24541 aatggtcgct cctgccacaa gtaacagtaa gcaggcaatc tttagagtcc ggcgagaaaa
    24601 acaaatacgg agaagaaggc gggcaaatgc gcgcagcatg gtcgttatcc ttaacgaaag
    24661 gggggaggtc gattttaggc gctaaagaaa gaaaagataa cgcctgacat cattttcaga
    24721 ttttgtcctg cccaagcgac aactgcgtta gccacagccg ggtgtcgaac tcaagctggt
    24781 ggtactgcgg ctccatatgg cagcacaatt gatagaacgc tttgttatgc tctttctcct
    24841 tcaggtgcgc cagctcgtgc accacgatca tgcgcaaaaa cggttccggc gcgttgcgaa
    24901 acacggtagc gacgcggatc tctgcttttg ctttcagctt gccgccctgc acacgagaaa
    24961 cagcggtatg taacccgagc gcgtttttta gcacatggat cttattgtca tacatcactt
    25021 tattgatcgg cggggcatta cgcagaaact gatttttcag atcctgagta tattgccaga
    25081 gagctttatc ggtggcgtag tcgtgtgttc ccggatagcg ttttgccagc acatcgccca
    25141 gacgctgttc gttaatcagt gttcgcacct gcgaaagcag ctgctcggga taaccctgaa
    25201 gataagtaag attgctcata accgcccacg aaaaagagaa aaagggtata ctcacgcacc
    25261 cttttcaggg gcaacgtcga aattttatca ttcaggaggg ccgatgagcc acttagacaa
    25321 cggtttccgt tcactgacac tacaacgttt tccggcgacg gatgacgtta acccgctaca
    25381 ggcgtgggaa gcggcagatg aatatttgct gcaacagttg gacgacacag aaatccccgg
    25441 cccggtgttg atcctgaatg atgcctttgg tgcgttaagc tgcgcgctgg cggaacataa
    25501 gccgtacagc attggcgact catacatcag tgaactggcg acgcgcgaga atttacgcct
    25561 aaacgggatt gatgagtcga gcgtgaagtt tctcgacagc accgccgact acccgcaaca
    25621 gccgggtgtg gtgctgatca aagtgccgaa aacactggca ttgctggaac agcaactgcg
    25681 tgcgctgcgc aaagtggtca cgtcggatac acgtattatt gccggtgcca aagcccgtga
    25741 cattcacact tccacgctgg aactgttcga aaaagtgctc ggcccgacca ccaccacgct
    25801 ggcatggaaa aaagcgcgcc tgattaattg cacgttcaat gaaccgccgc tggccgatgc
    25861 gccgcagacc gttagctgga agctggaagg tactgactgg actatccata accatgcgaa
    25921 tgtcttctcc cgcaccgggc ttgatatcgg cgcgcgcttc tttatgcaac atctgccaga
    25981 gaatctcgaa ggggagattg tcgatctcgg ttgcggtaat ggcgttattg gtctgacgct
    26041 gcttgataaa aacccgcagg cgaaagtggt gtttgtcgat gaatcgccga tggcggttgc
    26101 ttccagccgt ttgaacgttg aaaccaacat gccagaggcg ttggatcgca gcgagtttat
    26161 gatcaacaac gcgctctccg gcgtggagcc tttccgcttt aatgctgtgc tctgcaaccc
    26221 tccgtttcac caacaacatg cgctgaccga taacgtcgcc tgggagatgt tccaccacgc
    26281 ccgccgctgc ctgaaaatca acggcgagct gtatatcgtt gccaaccgcc atctggatta
    26341 cttccataaa ctgaagaaaa ttttcggcaa ctgcaccact attgcgacga ataataaatt
    26401 tgtggtgctg aaagtggtga agctgggacg tcgtcggtaa gattgggagt tacctgccgg
    26461 atgcggcgta aacgccttat ccggcctacg ttcagcacgt tacccccagg ccggataaga
    26521 cgcggcaagc gtcgcatccg gcaatgtctg ctcagttctg gaatcgcgct tccataaaac
    26581 aaaaaatccc tgtcaccatc ctctacattc tcttgtttag cgtttttcta cgtttattct
    26641 tccgtcacac agataaattc atccgttgcc aatctgtcaa cggatgttat catattgcca
    26701 atttgtcaac ggacgtgatg aagaatgcac ctgataactc aaaaagcatt gaaagatgct
    26761 gcggaaaaat acccgcaaca taaaacggag ttggtggctc tggggaacac gattgctaag
    26821 ggatatttca aaaaacctga gtcattaaaa gcagtattcc catctctgga taacttcaaa
    26881 tatctggata agcattatgt tttcaatgtt gggggcaatg aattacgtgt tgtagcaatg
    26941 gtcttttttg aatcgcaaaa gtgctacata cgtgaagtta tgacgcataa agaatacgat
    27001 ttctttaccg ctgttcatcg tactaaggag aaaaaatgat tgcgattgcc gacatcttgc
    27061 aagcaggaga aaagctaact gctgtggcac cttttctggc gggtattcag aacgaggaac
    27121 aatacaccca ggcgctggaa ctggtagatc atctgctgct caacgatcct gaaaacccct
    27181 tgctggatct ggtgtgtgcc aaaataaccg cgtgggaaga atcagcgccc gaatttgcgg
    27241 aatttaatgc catggctcaa gccatgcctg gcggtatagc cgtgattcgt acccttatgg
    27301 atcaatatgg tttaaccctt tccgatctgc cggaaattgg cagtaaatct atggtgtcac
    27361 gcgttttgag cgggaagagg aaattaacgc tggaacacgc taaaaaattg gcaacgcgat
    27421 tcggcatttc tcccgccttg tttattgatt aacgcgacgt gccggatgcg ttgcatccgg
    27481 cgcttcaaat aacgatttaa atctccagcg ccagccgtgt tccctgggca attgcccgtc
    27541 gtgcgtccag ctccatagcc acatcgcagc cgccaattaa atgcacagtt ttcccgctat
    27601 caatcagcgg ttgcgccagc gcgcggtttg gctcttgccc tgcgcagatc accacattgt
    27661 ccactgctaa tacctgcgtt tcgccgttga tcaccacatg cagcccgtca tcgtcaatct
    27721 tctgataact tacgcctggg atcattttca caccccgcga gagcagggtg gtgcgatgga
    27781 tccagccggt ggttttgcct aacccctgtc ccggtttgct ggctttgcgc tggagcatca
    27841 caatttgccg tgggctacgc gggatctgca ttccctgcgg gcttaagcca ccagcctgtt
    27901 gtaggctgct gtcgatcccc cactcattac agaacctggc gatattctgg ctggtggatt
    27961 cgcccggctg acttaaatac atcgccgtat caaaaccaat cccgccacaa ccgatgatgg
    28021 caaccttgtt gcctaccggc gctttgtcgc gcagtacatc gagataactc aataccttcg
    28081 gatgatcgat cccgtcgata ggcggaacgc gcggcacgat cccactggcg aggatcgttt
    28141 catcgaacgc ctgtaactga tccgccgtca cggtgtgatt gagtttcagc gtcacgcccg
    28201 tcacttcgat catccggcgg tagtagcgca gcgtttcgta aaactcctct ttgccgggga
    28261 tctgtttggc gatattaaac tgcccgccga tctcgctgtg cgcatcaaac aatgttacct
    28321 gatgcccacg cgccgccgcg ttaatggcaa acgccagccc agcaggtcca gcgccgacca
    28381 ccgccagatt ttttttctgc acggcgggaa ggattggcat tttggtttcg tggcaggcgc
    28441 gaggattcac caggcacgag gtgactttgc caacgaagat ctgatcgagg caggcctggt
    28501 tgcagccaat acaagtgttg atctcatcgg ctcgccctga ttgcgctttt gacagcagtt
    28561 ccgcatccgc gagaaacggt cgcgccatcg atactatatc ggcatcgccg cgcgagagaa
    28621 tatcgtcggc aacctgcgga tcgttaatcc ggttggtggt taccagcggc agcgagacgt
    28681 ggcctttcag tttgcgcgtg acccagctaa atgcgccgcg cggcacgggc gtggcgatgg
    28741 tcggaatccg cgcttcgtgc cagcctattc cggtgttgat aatggtcgcg cccgccgctt
    28801 caatggcctg cgccagttct accgtttcgg caaaagtgcc gccgccttcg accaggtcga
    28861 gcatcgacaa ccggtaaata ataatgaagt cgttgccgac gcgttcgcgc accgtacgca
    28921 ctacttctac ggcaaatcgc atccggttgc ggtaatcgcc gccccactgg tcactacgct
    28981 gattggtgcg cagcgtcaga aattcgttga tcaaataccc ttcagagccc atcacttcga
    29041 cgccatcgta tccggcctcc cgcgccagtt gcgcgcagcg ggcgaaatcg tcgatcagtt
    29101 gcaggatctc ttcatgggtg agttcgtgag gaacgaaacg gttgatgggg gcctgcaatg
    29161 cggacggtgc gaccagatgc ggttggtagc tgtagcgccc ggtatgcaaa atttgcaggg
    29221 tgattttgcc gccttcctga tgtacggctt cggtaatggt gcgatggtgt gggatctggc
    29281 tggcgtcgtt gagcattgcg ccaccttcca tgccaacgcc tgttaaatct ggtgcaatgc
    29341 cgccgctgac aatcagcgcc acgccgtgac gggcgcgttc ggcataaaac gcagccagcc
    29401 gttcagcacc gtccgggtat tcctccagcc cggtgtgcat tgaacccatc aacacgcggt
    29461 tttttaacgt ggtaaaacct aaatccagcg gggcgaacag cgacgggtag ctcataaaat
    29521 tgtccagtat gtaaaataat tgttatgtgg tcggatgagt tctaatttag ccgtcgatgg
    29581 agaaaaggga aaaggggagt gcggggtttg tgatgggatt caaaaagaat acgcccggtg
    29641 gtaacgccgg atgcgacgct tgccgtgttt tatccggctt gggggattgc acatgtaggg
    29701 cggataaggc gttcacgccg catccgccag ccgttgcaaa ttactgctta cggaaaaaat
    29761 cgttatacaa catatacaaa tgagcggcac tccaggagaa atttggtgcg ccttgctgcg
    29821 cgcctgtcag cgggttgtaa ttctcctgaa ttgggccatc ggcggttaat cctttggcgt
    29881 gcctgaagaa cgtatccgcc agtttcaggg catcatcacg ataaccgtaa cgctccatcc
    29941 ctttcagacc aaaccagaac tgatccaccc atacgcgccc gcgccagtag atatcagcgc
    30001 caaaagccgg attggttaac gccgccgttc ccagcggaac aaaggtgttg aactctttag
    30061 gatcgagcat caccttcacc accgcgtcgg cattggcctg cgttgccgca ccgttaaaca
    30121 gcggcgacca gccttccggc cctttaccgc gctcaacaat cggtttgccc gcgcagccgt
    30181 tcgccagtgg tttatcttca atacgtacgt catagtagaa ctgggtagtc gggtcgaaca
    30241 tacaggtgtt gatgtagtcc gcgagctgct gtgccaactg gcgatagcgt ttggcctctt
    30301 ctggcttacc gaggatggtt gccatctccg ccagataatg gttatcgctg tacatatagc
    30361 tggcctgatc caccgactcc tgcaatagcg agtagcccag caacgttccg tcctgactgc
    30421 ggttttcggc gaatttcacc gtccagtcgc tacgtttgcc gccattagcg acatatttat
    30481 ccaactgttc tttgtcgata aaaccaaaga cggcggcatc atcacgcccc gattcccacg
    30541 acgcagcgac ctgtgccgga atttccagac tgtcgtactg gcctttctcc accacgcggg
    30601 cgtagttgtt cagcccagac tgcgtctctt ctttatcgcc ttttttcact gtaaacagca
    30661 tctcgccgct ctcggtgttg tgggctttgt cgcgggtcgc gccatattcc ggcaccccgt
    30721 tgccgttatg atcgcggtta cgtaaccacc agtcgtgata ggccaccagt ttcgggtaca
    30781 tctctgccaa ccaggcttta tcctaggtga cgttgtatac ttccatcacc gaccaggcgg
    30841 caaggctggg tttggtatta cgttcgttcc agttgccgcc atcgccacca cgctcggggc
    30901 taaggttcca cgctatcagg tcggggacaa agcccacatc ctgcggacgc acgctgtcgc
    30961 caggctggat ctgccaggag aagaccgcgc ggatattctc tttggcgata tccggattga
    31021 aatgcaccat cgcaaacgcc tgcttccagg tatcccacgg ccaggtctga ttgccggaga
    31081 accagcgtcc ggtcaccgac ggcgtgacgg tgttataatt caccgcaccg cccggtgagc
    31141 gccagttacc gttgagcgtt tcgatcgctt tcaccgcgac gcgcgtctgt tccggcgtcg
    31201 catccggatt ggttaaccct ttcttcaggt attcttccca gcgttgctgc gaggcggtga
    31261 gataaaacgc cggacgcgcc agaatatcgc ggatctgcat ttgctctttg ctaacttcct
    31321 gagcggtcag cagatgggaa taggtggtgt agagcgtggt cgaaccgttg atatgcgcct
    31381 tactggtaaa gcgattaccg ttgatttcag tctgcaccgg cagggattta tgcacctgat
    31441 attccgattc gccggaggtc agcagatccc aggtggcgcg cactttgcca aaggtgactt
    31501 tcaggccatc acgggtggcg ctgattttgc gctgatagtc agggtattcg ccagcaatgg
    31561 ttttatcgga aagcggtttc ccttctttcg cttccagttt ttccagcagt tcgccatccc
    31621 acaccagatc cagtggttta ttgctgatga ttttagtttc cagtagtgac gtgcgcggcg
    31681 tggcgaagcg cagagtcatt tcgacctgca catcttttgc tgtcagtttt tgcaccagcg
    31741 cgccgggaat actgtatgcc tccagcgtga agttgacttt cttgccgtct tgccagacag
    31801 tcaggcggtc gaaattgctg gccataaagt tgatgtattc ttccgtcagc agcgcaacgc
    31861 ccggaaagcc gcccatggtg ttcgggccgt ctggcaacag atgaccatgc caggctccga
    31921 gatcgaaaaa cggattaaaa cgctggtgat cgtcgtaatc gtaatccttc atgtactgcg
    31981 gcgcaccagt acggttaatc acgtttttat aattgtcggc gttagcggca tgagcggaaa
    32041 aacttatcag cagagcgcag gttactggcg ttaaaatagt tttgattttc atgaaattac
    32101 agtccttatt accagtagaa atattgcatc aggaagattt ggtcgctggg atcgttagag
    32161 gcatttttca tgtagaagcg gctatcaaaa gccgtggcga aacgtccgcc gccatattcg
    32221 taccagatac caaactgcat gaaagaggtg gtttcatcgt cagcgtggtc agggcgatgt
    32281 ttggcgtagg tgtaagcggt ggagagataa acgcccttta acgcttcata catggcgctc
    32341 accatcatcc cgctttcact tcccggtacg tccagcccgt cggcacgtcc ggtatggtgc
    32401 caggcgcgag cggcaaagtt aggggtaaac aggcccgtga gatagagttg tcctgtgccg
    32461 tcggtaattt ccagaccatt catccaggtg atgccttcgg caaggtcata ctggatgtaa
    32521 ccgttcatcc attgccggat aggtgtattt gtcgtcgtag cgatcgtact tgccaaaatg
    32581 tagccaggct ttgctttcgt catgatgtcc ggcaggtgtg gcggtgacgc tataacgaaa
    32641 gttgccgctt aagttctgta ccttcagcgc ggtcataatg tcgcgggtat tgggaatgac
    32701 gtagcccaaa tcgggtgtga agtcgcccca ccattgcagg tcatccagcg aggaatcctg
    32761 acgcagactg agcatgattt cggtgccatc attggtacga taaccgccat agaaacggtt
    32821 aatcccacct tcgaaaccac cccagctatg gtcaggcacc caggcattac cgtcgtggtc
    32881 ggcctgcacg gtccagcctt cgccgtaaat caggccgaac caggagccgt acttcatctc
    32941 caggccacct tcaatatagg tgccgtcgct gtatttatgt ttattctcac cctctaaatc
    33001 catatatcca ccaagcccta attcgccgta aaaacgaaat gaaggagaag atgtcgaggt
    33061 aattgcgggt gtttttgtgt cgtcatgttc tgccggacgt gcttcttctc ctgccgcaga
    33121 aaaggcgtag caaaacggca gagcaagtaa tgctctgcat ggtgcagtga ttaatttcat
    33181 tgtgttaatc catgagctaa agaaaaagta cagcctttta tcgttgtaat tataaaaata
    33241 taaaggcaga aactattttc cctctcctca tccaggaaga gggaaaatgc ctgctcattt
    33301 tttattagtc tgcttaatca acattgctgg ctggcgtcgc ttcaatatgg cgctcttcag
    33361 ccgttaatcc ctttatatat ttactgtatt tccaccacgc aaagccgagg aagataacaa
    33421 taccgccgac gttataaaaa atgatggtca gaatattcgc gccagtcggg aatgtcgaag
    33481 cgacaaatcc tacggcaaat atcgcaatca gcattgaaac aacgataata ccggtgcggc
    33541 gggagcccat acggaaatcg cgtggcaggt gatctaattt ggcgcgtaaa ttcaggtaag
    33601 ccagcatgat gaataacggc ggaagcatgg acgctgcggc ggtcatatta ataatggtat
    33661 tcatcagatc ctgcacggta ttggaaccga gcatcgggat aatcatcagc gggatgacga
    33721 tcagaaactg gatccatgct gcgcgcgccg gaacgccgtt ttcgttaagt tcgacggttt
    33781 tcttaccaaa gatgccttcc gggatttcgg agaagaaaat tttcaccggc gttgcggtcc
    33841 acatcagcag ggaaccgaac attgcggtaa aggacaccag accgacaaag cgattcatca
    33901 acgcttccgg cagaccaaaa tacgccgcca tgccgtggaa tacctgcacc gatccgccgg
    33961 taaatttcaa ctctttgctg ctgacgaaga cgttaatcag caccgaggag acggaataca
    34021 gtacgccgat aaaaatcccg gcgaggatga tcactttaac gaacgatttc gaaccgcctt
    34081 tgacgtcgtt aacgtacacc gcgacggact ccgcgccacc tgcggccata aagatccagg
    34141 tggtaacgcc gaggaacgcc cagttgaagt tcgggatcat cgcgtcaacg gtgatcggat
    34201 cggcaggctg tacgccgcca accagtgccg tacctgccag taaaatgtag gagagcgtta
    34261 acagcagcat cagcgttgaa gtgacggagg taattggccc cagcattttc gccccgttgg
    34321 tggaaaccca ggtggagaag gcgaacagca ccatactgat aacggtggtg gcaaccggcg
    34381 tcataatata ttcgtagccg aggaaggcat acgaagcata agcaataacg cgcggcaaca
    34441 gcgaggtgaa aaagaacaga tttacgaacc agtaagtgta ggcagtaata aatgcccaac
    34501 gtccgcccag cgaacttttt acccacgcgt agacaccggc ttctgagttt ttatttaacg
    34561 aaacaaattc tgcgatgatc agacagaagg gaataaaata aaaaatcgtc gcgaggaaaa
    34621 acatcggtgc cgaggccagt ccaagctcaa tattattatt gataacgttg ttaaagctgt
    34681 aaacggcggc aaaagtcagc gagagcaagc cgaatttgcc gattgtatta cgtttggtat
    34741 cagacataac acatctccga aaatgtgtag ggtagcggtg gcggcaaact ccttttgccg
    34801 ccgtagttgt tatttgttat ggaaataacc atcttcgatg gtgactttga gaaccacttt
    34861 tttgaccgcg ttattgcaga taaaccgata cgcttcatgg atatcgcaaa taacgatttg
    34921 cccttcgtgg acctctacgg tttctccgca gccttttaaa tattcacggt cagtttcatc
    34981 acgataatat tccactacct gtaatgtctc tttcggcgca tattcaattt tttgctgccc
    35041 ttgcaggtag taatgcactt caaaatagcg tcgatgcccg gtaaatagcg catcggtcgc
    35101 ggagtcattt tccacacggt aggtcaatga atcaccgatg gagtgggcga cgccaggctg
    35161 aatgttgtcg atattttcaa tcgcttcaac gcagcgttgc cacttcttgc cagaggcgta
    35221 aatctggcgg aactgttcta agttatcgat gatcctcatt gcttattctc gctgtgcaaa
    35281 ttcgtggaaa agaaccctgc gccgaactca tacgacgcca ggctttgcgc ggtagcttct
    35341 ccgccagaaa ccggcagcaa cgtaaagccg tagctgaagt cacggaacca gacgcgccag
    35401 gagtccagca cctcgctgcc ccaggagttg gagccgaggc caagtagctg gtggtcgagg
    35461 ttcagggtga tgtcatcact gcgctgcagc tcgttacagt gctgggcagc gtgaatgttt
    35521 tcctgggtat agtgccaggc gctgaagtta attgggcgct gcggaaccac cagcagaccg
    35581 ttgccgtggc ggttagtcag tgccgtccag cggacatgct gacggttgcc gttgttctgc
    35641 gggaagggat agttctcgaa catggcatcg acggtgctgc gccagatatc gatgatgtta
    35701 gcctgctggc tgtcggcgta gttttcgccc ggtccacgac cgtaatacgc cacctgatcg
    35761 tattcgccgt taatccccat ggtgaaaccg atgcacggaa tgatgtgcgg atagtcgccg
    35821 taacgctcgc cggaaagcgc cacgttaacc tggccatcgg cagcgatgcg ccagatgtag
    35881 gtacagcgca tcccgaagtc aaacaccggc ggtgcaataa ctgtgcggct gatgatcaac
    35941 acttcaccat cgctctgttc tacggcaaag tcgcgcagat gttcctgcat gatctgcaaa
    36001 tgattcggct gccacaaccc ttcgtactcc tgcttgtggt tgtcgatcat cggcttgaag
    36061 aagttgatct ttggctcgcg ggtcagcagc gactcgccat ttacctgcca ggatgtcggt
    36121 ttgccactca tttttgagaa ggtgatcgcg aagttgtagc cgcgaacggt gcagctcaaa
    36181 cgatcgtctt ccagcgtcag cggacgcgca ttatttggtg cgaaaggcac tggctgcgcg
    36241 gtgttttcct tcagcgggaa ctgataagtg gcgatagaat gcccggcttc gctgtagcgg
    36301 gtgtgggaat ctttggtcac cgtaatgttg aggaacgctt cgcgggcgtc cagctgcggc
    36361 agcgtgatct gcaagggggc ttcgctgttc ggcgcaacgt cgcgcagttt aatctgctgc
    36421 gtcgcgagcg tttcaccttc ggcgcgcacc tctgcgtgca gggtgtagtc atcaagcgtg
    36481 gtaaaccaca gtttattttc gactttcaac tcgccgcgag tcagatcccg cgcgtggatt
    36541 tttaccggcg cgataacctg tttgtactct ttcaggcccg atcccggcgt ctgatcggaa
    36601 tagatcaaac catcaagaca gaagttatag ttgttgggat agtcgccgta gtcgccgccg
    36661 aatttatacc agacattgcc gtggtcgtcc tgtgcctgga tcccgtggtc gcaccactcc
    36721 cagacataat gaccctgaat gcaatcgtgc ttatagaaga cgttctggta ctccgtcagc
    36781 ccgcccggtc cgttccccat cgcatgagca tattcacaga tgatgcgcgg cttcggatgc
    36841 gggtattcac caaactcatt catcagcggc acgcgggtgt acatggtgga aataatatcg
    36901 accacttcag catcgcgatc ttcttcgtaa tgcaccagtc gcgtgtcatc cagcgctttc
    36961 gccgcatggt acatcgcgcg gatgttacag ccatagccgg attcattgcc cagcgaccag
    37021 atgatgatcg acggatggtt tttctgtgcg tggatatggc gaacaatgcg ctcgacgtag
    37081 actttttccc actgcggatc gtcggtaata cggctgatat cgccgacatt agcaaagccg
    37141 tgcgattcga cgtcggtttc cgccatcaca aacaggccgt agatatcaca cagttcgtaa
    37201 aaacgcggat cgttcgggta gtgagcggta cgcacggagt tgatattgtg ctgcttcatc
    37261 aactggagat ctttctcgac gcgatccatt ccaacggcgc ggcctttgcg atgatcgttg
    37321 tcgtgacggt tgacgccgtg cagcatcaca taacggttat tgatccagaa cagaccgtcg
    37381 cgcactttga tatcacggaa gccaacgcgt tgtggcacca cttccagaac gttgccgttg
    37441 gcgtctttca gcgtcatgac cagatggtaa agataagggg attctgctga ccattgctgc
    37501 ggctgttcga cagtaaaagc aaagctggcg ctggtcagtt tttcaattgc caaatgatca
    37561 atggcgctgc tgtgcaccac gcgttcgcca tcaaacaggg tatattccag cgtcgtgacg
    37621 acaggggagg cggcgagatt ttccagcacc acttcgcagg aaagcgtggc atcgcaatag
    37681 gcttcgtcaa agtcggtacg cacagtgaaa tcgttaatat gcgttaggtg ttttccgacc
    37741 agataaacat cgcggaagat ccccgccgac caccacatat cctggtcttc cacgtaggta
    37801 gagtccgccc actgcatcac gcgcacacac aacaggttgt cgccggtttt aaccatcgcg
    37861 ctgatgtcaa actctgcggt caggcgactg cccttgctga aacccacata ctgaccgtta
    37921 acgtagactt caaaataggt ttcgacgccg tcaaatttaa tcagcgtctg tttaccctgc
    37981 cagccgtcgc tgagggtgaa aatacgttga taggcaccgg ttgggttatc gctggggaca
    38041 aacggcacat cgatggggaa cggaaaacct tcgtcggtat attgcagttt gccgtgacct
    38101 tccatttgcc acatggcggg gacggtaata tgcccccagt cagccattaa ctcagaggtg
    38161 aaggcttctg gtacttgcag cggatggtca aaaaagtgga aattccactg accgcttaag
    38221 ggcagaaaca ggctgctggt ttcgcgggca aaggtacgcg cttgcgcaac agaatcatat
    38281 gaaaaaaagt acgcacgcgg cgcaagtcgg ttttcgtggg tgagctgaat gttttcccag
    38341 cgattcatag ggcctcccgg tagagagaaa tcggaaccat tcatggtgat ggtagtttgt
    38401 gtaattgctg gggagtttag taaaattttt actaaaaagt agcaggtaaa tgcatttact
    38461 ttaactggat cacgaaaata aacggcagca ctgtccggca aaaatgattg ccggacagtg
    38521 tgtaagggga tttaacgggt cgtgccgcgc agttttaatt tgctgggaac gaagactagc
    38581 agcggcagcg cgcgaccatc gcgggctttt tcatacacca ggttaacgcc ctgacttccc
    38641 atcatctcgg aatggatgcg cacggtggag agcggcggaa aggtaaatcg cgcggtgggg
    38701 atatcgttaa cgctgataag cgaaatatcc tgtgggatgt tcaggccacg ttcatgaatt
    38761 gcccgcagta cgccgatagc aatggaatcg gaagcaacaa acagtgcctt cggatagtct
    38821 tcccgcgcca gcatttgttt tgccagttca taacccgacg aactggaaaa accgccgcgc
    38881 cagatatcct cttcgcgtac cacttgtttc agtcggccat attccgcaaa ggcgacctca
    38941 cgaatatccg ccttgccagg ctcatcttca ccgccaataa aaccaatacg attaacgccc
    39001 tggttgatat agaagtcgat gatttcttta ctgatgcgtg ccagatcgat atccaccgca
    39061 tcgtaaccgc tgccgggttc gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg
    39121 gcagcggcgc gcagggcggg cgtgggtttg ccgacaatta aaataccggt gacgttttta
    39181 atgtctggta atccgttgtg ttcataacag ttggtcagtt cgatcgccag cttttcgcac
    39241 tgagtttcaa tgccgtggcg gatcgccaga tagtaaggat cgttgatctc cagctcctgc
    39301 tggtagctgt agatagccag aatatggtgt tggttgactg cgcctgtctg tagtttacgg
    39361 gcgctactgg tcttgtactc cagcttttcg gcgatctcga gaatgcgatg tttcgtctct
    39421 tctttcacat tcaatgtcgg atcgtcattt aagaccctgg atactgtcgc cagggatacg
    39481 ccagcttcga ttgcgatgtc ttttaatgtt gccatgttta ccttttttac tttccctgat
    39541 gtgcgccccc attgtagagg aagtgcttat cgggtcacct gttttcagta aataaagcgc
    39601 gtttctttga cgctgtaacg gtaacacagc ggcttaggaa tgtgatcacg cctgcattct
    39661 ccatttttag cctacaggat tgagaaaaac ttttgctata gtttagtaaa attttacccg
    39721 aagggaggaa gcaatggaaa ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt
    39781 cggaaagatt gtggaagtga aacgccatga aaacgccgac aagctgtaca tcgtacaggt
    39841 tgatgtgggg aaaaaacact gcaaaccgtg accagcctgg tgccgtacta cagcgaagag
    39901 gagctgatgg ggaaaacggt ggtggtctta tgcaatctgc aaaaagcgaa aatgcgtggt
    39961 gaaacgtcgg aatgcatgct gttgtgtgcg gaaaccgatg acggcagtga aagcgtgttg
    40021 ttaacgccgg agcggatgat gcctgcgggc gtacgcgtcg tataaaaaga tcggatggcg
    40081 acgtcgtatc gccatccgat ttgatattac gcttcttcga cacttactcg catggccgcc
    40141 agcgccttac gcgccgcttt gatcaccagt tcacactgtt caatggtcag tgtcagtggc
    40201 ggttcaatgc ggatcgtttt ggcgttattg agcgttccgg ccaccagtac gcgctggcgg
    40261 aacatctcgc tggcaaagtt atagccgatt tcgttatcaa caaactcaat cgccatcaac
    40321 atccctttac cacgcgcttc ctgtaccaga tcgggatatt cccgcgccag ttgacggaaa
    40381 ccatccagca acatatcgcc tttttgctca gcctgagccg gtaagttctg ctccagcaac
    40441 acattgatgg tcgccagcgc cgccgcacag gccagcgggt tgccgccaaa ggtggtggta
    40501 tgcaggaatg ggttgtcgaa cagaactgaa aacacctctt cagtggcgat ggtcgcgcca
    40561 atcggcatca cgccgccgcc gagggctttt gccagacaca gaatgtccgg ctgaacgttc
    40621 tcatgttcgc aggcaaacat cttgcccgtg cgccccatgc ccgtttgtac ttcatcgagg
    40681 atcatcagtg cgccgaactc atcgcacagc ttacgcacgg cggtgagata gcccggcggc
    40741 ggcagaatta cgccaccttc gccctgaatc ggttcgagga tcaccgcagc cacatcatca
    40801 ccggtttttt tgcactcatt aagagccgtg cgcatggctt cgatattgcc aaacggcaca
    40861 tgacggaacc ctggcagcag tggcataaac ggtttgcgga aggtcgattt cgccgtggcc
    40921 gacagcgcgc caagtgattt accgtggaac gcgccgctgg tggcaataaa agtaaatttg
    40981 ccgcgcggcg actgataagc cttcgccagc ttcagcgccg cttcaacgga ctcggtgccg
    41041 ctattacaga agaagctgta tttcagttta ccgggcgtta gcgcagcaag ggttttcgcc
    41101 agcatagccc gtaacggatc gagtagctcc tggctgtgca gtggttgttt cgcaagttga
    41161 ttctgtacgg cggaaaccac aactggatta cggtgcccca cgttgaaaat tccaaaacct
    41221 cccaggcagt cgataaactc ctgtccctgg gtgtcgacaa gcgtatttaa acctcccgct
    41281 tgccactcta cggctccgta atccccgccg gcggtaacag atttgcgata ctctaaaaac
    41341 cccggattga catgctcttt gaagtattca atcacctctc ggttaagtgc tttcatctcc
    41401 tcatgatcca gcgttcgctt ctcaatgaga ttcagggcgt gggcgctgca cgctaaagcc
    41461 gatgcgctcg aaggtaacct gttcaaaata tgctccggag gctcgcgtat cacatgatac
    41521 cgatttaagt attgcaggga ttgcgccact ccggctgcga tcgcgaaaat cgggataaac
    41581 acaaatgcgg ttatcatttg tgcaatattt aatgctatcc gttaacattt gttgttattt
    41641 tgcacggttt ttgtcgggta tggatttgat gattgctgcg caaatgcacg ttttcagggc
    41701 aataattgcc tgatttttgg gcaagataat taacgttttc gtatagtaac agagtgaaag
    41761 tattttaaat caaagaatta gatttgtgct ttaatcgcaa attgcgatct aaatcaaatt
    41821 aatcggttaa agataaccgc agcggggccg acataaactc tgacaagaag ttaacaacca
    41881 tataacctgc acaggacgcg aacatgtctt ctcatccgta tgtcacccag caaaataccc
    41941 cgctggcgga cgataccact ctgatgtcca ctaccgatct gcaaagctat atcactcatg
    42001 ctaatgacac ttttgtgcag gtgagcggct ttaccttgca agagttacaa gggcagccgc
    42061 acaacatggt gcgtcacccg gatatgccaa aagcggcgtt tgcggatatg tggttcaccc
    42121 tgaaaaaagg ggagccctgg agcggcatcg tgaaaaatcg ccgcaaaaat ggcgaccatt
    42181 attgggtgcg ggccaatgcg gtaccgatgg tgcgcgaggg aaaaatcagt ggctatatgt
    42241 cgattcgtac ccgggcgacg gatgaagaga ttgcggcggt ggagccgctg tacaaagcgt
    42301 tgaactccgg acgtaccagt aagcgtattc ataaaggcct ggtggtgcgt aaaggctggc
    42361 tgggtaaact gccttcatta ccgcttcgct ggcggacgcg tggagtgatg accctgatgt
    42421 ttatcttgct ggcggccata ctttggtttg ttgctgcccc ggtggtgacg tatttcctct
    42481 gtgtgttagt ggtattgttg gcaagcgctt gttttgaatg gcagattgtg cgcccgatag
    42541 aaaatgtcgc ccgtcaggca ctgaaggtgg cgaccggaga gcgtaatagt gttgagcacc
    42601 tgaatcgcag cgatgagctg gggctgacat tacgcgcggt agggcagctt ggcctgatgt
    42661 gccgttggct aattaacgat gtctcaagcc aggtgtccag tgtcagaaat ggcagtgaga
    42721 cgctggcgaa aggcaccgat gaactgaacg aacataccca gcagacagtt gataacgttc
    42781 agcaaacggt ggcgatcatg aaccaaatgg cggcgtcggt gaaacagaac tctgccacgg
    42841 cgtcggctgc cgataaactt tctatcaccg ccagtaatgc ggcagtgcag ggtggggagg
    42901 cgatgaccac ggtgatcaag acaatggacg atatcgccga cagtacccag cgcattggca
    42961 ccattacttc gctgattaac gatattgcgt ttcagaccaa tattctggcc ctgaatgcgg
    43021 cggtggaagc ggcgcgtgcc ggcgaacagg gcaaaggttt tgcagtggtg gcaggggaag
    43081 tgcgtcattt agccagccgc agcgctaatg ctgccaacga tattcgcaag ctgattgatg
    43141 ccagtgctga taaggtgcaa ttcggttcgc agcaggtaca cgccgccgga cggacgatgg
    43201 aagatattgt ggcacaggtg aaaaacgtca cccagttgat cgcccagatt agccattcaa
    43261 cgctggaaca ggccgatggg ctttccagcc tgacccgtgc agtggatgag cttaacctga
    43321 tcacccagaa aaatgccgag ctggtggaag agagtgcgca ggtgtcggcg atggtgaaac
    43381 accgcgccag ccgactggaa gacgcggtga cggtgctgca ttaatcggta tgccggatcc
    43441 ggcggttgga gcacaatgcc tgatgcgatg ctggcgcatc ttatcaggcc tgcggggtgt
    43501 gtagcgggtg taggccggat aaggcgcgtc aagcgtcgca tccggcaatt gcaccgcgcc
    43561 actggcggat gcggcgatta atccagttgc gtaatatcaa aagcggcgcg gtcgatcacc
    43621 gcaatgatct tttttatttg tgcatcactg atatcgctct ggttgacgcg taaatccagc
    43681 accgctttaa aattatccag cgcccgcttc atttgcgggt tctggcgcag cgccgcgcca
    43741 acgcaacgcg ctttgatgcg ttcttcaatc atctccacct gttcgcggtt ttcttccagc
    43801 cactgcgcgc cttgttcggt cagcgcaatc tgcttcttac caccttcctc ttcgcggatg
    43861 gtaatcagcg actgctcctg cagaaaatcc agcgtcgggt agatgacgcc cgggcttggg
    43921 gtgtaattcc cctgggttag attctcaatc gctttaatca attcgtaacc gtggctgtca
    43981 tcgcgcgaga gaatatccag aatcaccaga cgcaattcac cgtgaccaaa gaaacgttgc
    44041 cgacgaccgc cgccgcgacc gtggcgacca ccgcaacatt gaccgtgttc atgctggtga
    44101 ccgtgtccgc agtgctcgtg ttctgacttc tcacctttgc agcagccctc atggcgtggc
    44161 tggccttcat gtttacaaca cccttcgtga tgatggctca tatcagcctc cttttgcttg
    44221 attagatata tctaatttat atctgatgtg agcattactg caagtacaaa aatgattttt
    44281 aaacgtggaa gcacctcaca tctaggccgg ataagacgtt cacgccgcat ccggcagcat
    44341 tgctatggca ctgaatgcct tcacatactt cagacatatc agttctacaa atcgcttgca
    44401 tttatcatga ttaacaatca ttatcatttg cgaattttat ttagatatat ctgattacca
    44461 tcacgaaggc gataacaatg aataacaccc cccgctaccc gcagcgcgtt cgcaatgatc
    44521 tgcgcttccg tgaactgact gtgctacgcg ttgagcgcat cagcgccggt tttcagcgca
    44581 ttgtccttgg cggcgaggcg ttggacggtt ttacatcgcg cggctttgac gatcacagca
    44641 aactcttctt tcctcaacct gacgctcact ttgtgccgcc aacggtaacg gaagagggca
    44701 tcgtctggcc ggaaggacca cgcccaccgt cgcgtgacta tacgccactg tatgacgaac
    44761 tacgccatga actggcgatc gatttcttta ttcacgacgg tggggtcgcc agcggctggg
    44821 cgatgcaggc gcaaccgggc gataaactta cggtggcagg tccgcgcggt tcgctggtgg
    44881 tgccggaaga ttacgcgtat cagctgtatg tctgcgatga atccggaatg cccgcactgc
    44941 gccgccgcct ggaaacgttg agcaaacttg ccgttaaacc gcaagttagt gcgctggtta
    45001 gcgtgcggga taacgcctgt caggattatc tcgcgcacct tgatggtttt aatatcgaat
    45061 ggctggcaca tgatgagcag gcggtagatg cgcgtctggc gcagatgcaa atccctgccg
    45121 atgattactt catctggata accggcgaag gtaaagtcgt taagaattta agccgccgct
    45181 ttgaagcgga aaagtatgac ccacagcggg tacgtgcagc ggcttactgg cacgcaaagt
    45241 aactgatata taaaaagaaa aaggctgacg atttctcgtc agcctttgct atatctggtg
    45301 gcccctgctg gacttgaacc agcgaccaag cgattatgag tcgcctgctc taaccactga
    45361 gctaaggggc catggtagcg gattataaag taactccgtg tcgcaatcca gccattaccg
    45421 cgcgcctgct gtttttataa acaatgtatt ttcaatccgt tatacttttc ctggtgatgt
    45481 tagaaaggag taaatatggt tgaggatatt ttggctccag ggttacgggt cgtgttttgc
    45541 ggtatcaacc ctgggctttc atccgccggg actggttttc cctttgctca tccggcaaat
    45601 cgcttctgga aggtgatata tcaggccggg tttaccgacc gtcagttgaa gccgcaggag
    45661 gcacagcatc tgctggatta tcgttgtggc gtcaccaaac tggtagaccg tccaacggtg
    45721 caagccaatg aagtttcaaa gcaggagctg cacgcaggtg ggcgtaagct gattgaaaaa
    45781 attgaagatt atcagccgca ggcgttggcg attctgggca aacaagcata tgaacaggga
    45841 ttcagccagc gcggtgcaca gtgggggaaa caaacgctca ccattggttc gacgcagatt
    45901 tgggtgctgc caaatcccag cggtttaagt cgcgtttcac tggagaaact ggttgaagcg
    45961 tatcgcgagc tggaccaggc gctggtagtg cgtgggcgat aaaaaacgcc accga
//
LOCUS       DADLSB010000015        42007 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.015, whole genome shotgun sequence.
ACCESSION   DADLSB010000015 DADLSB010000000
VERSION     DADLSB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.015"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            121..2655
                     /gene="mrcB"
                     /locus_tag="JN331_000844"
     CDS             121..2655
                     /gene="mrcB"
                     /locus_tag="JN331_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY7259058.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2875..5118
                     /gene="fhuA"
                     /locus_tag="JN331_000845"
     CDS             2875..5118
                     /gene="fhuA"
                     /locus_tag="JN331_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY7259059.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKNGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5169..5966
                     /gene="fhuC"
                     /locus_tag="JN331_000846"
     CDS             5169..5966
                     /gene="fhuC"
                     /locus_tag="JN331_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY7259060.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5966..6856
                     /gene="fhuD"
                     /locus_tag="JN331_000847"
     CDS             5966..6856
                     /gene="fhuD"
                     /locus_tag="JN331_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY7259061.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6853..8835
                     /gene="fhuB"
                     /locus_tag="JN331_000848"
     CDS             6853..8835
                     /gene="fhuB"
                     /locus_tag="JN331_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY7259062.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8870..10150)
                     /gene="hemL"
                     /locus_tag="JN331_000849"
     CDS             complement(8870..10150)
                     /gene="hemL"
                     /locus_tag="JN331_000849"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY7259063.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEESGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10375..11796
                     /gene="clcA"
                     /locus_tag="JN331_000850"
     CDS             10375..11796
                     /gene="clcA"
                     /locus_tag="JN331_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY7259064.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11820..11885
                     /locus_tag="JN331_000851"
     CDS             11820..11885
                     /locus_tag="JN331_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259065.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            11878..12222
                     /gene="erpA"
                     /locus_tag="JN331_000852"
     CDS             11878..12222
                     /gene="erpA"
                     /locus_tag="JN331_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY7259066.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12269..12892)
                     /locus_tag="JN331_000853"
     CDS             complement(12269..12892)
                     /locus_tag="JN331_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY7259067.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12930..13730)
                     /gene="btuF"
                     /locus_tag="JN331_000854"
     CDS             complement(12930..13730)
                     /gene="btuF"
                     /locus_tag="JN331_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY7259068.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13723..14421)
                     /gene="mtnN"
                     /locus_tag="JN331_000855"
     CDS             complement(13723..14421)
                     /gene="mtnN"
                     /locus_tag="JN331_000855"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY7259069.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGCLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNIPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14505..16022
                     /gene="dgt"
                     /locus_tag="JN331_000856"
     CDS             14505..16022
                     /gene="dgt"
                     /locus_tag="JN331_000856"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY7259070.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRINLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16152..17576
                     /gene="degP"
                     /locus_tag="JN331_000857"
     CDS             16152..17576
                     /gene="degP"
                     /locus_tag="JN331_000857"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY7259071.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17731..18887
                     /locus_tag="JN331_000858"
                     /pseudo
     CDS             17731..18887
                     /locus_tag="JN331_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(18976..19362)
                     /locus_tag="JN331_000859"
     CDS             complement(18976..19362)
                     /locus_tag="JN331_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY7259072.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19676..20500)
                     /gene="dapD"
                     /locus_tag="JN331_000860"
     CDS             complement(19676..20500)
                     /gene="dapD"
                     /locus_tag="JN331_000860"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY7259073.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20531..23203)
                     /gene="glnD"
                     /locus_tag="JN331_000861"
     CDS             complement(20531..23203)
                     /gene="glnD"
                     /locus_tag="JN331_000861"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY7259074.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23265..24059)
                     /gene="map"
                     /locus_tag="JN331_000862"
     CDS             complement(23265..24059)
                     /gene="map"
                     /locus_tag="JN331_000862"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY7259075.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24427..25152
                     /gene="rpsB"
                     /locus_tag="JN331_000863"
     CDS             24427..25152
                     /gene="rpsB"
                     /locus_tag="JN331_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY7259076.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25410..26261
                     /gene="tsf"
                     /locus_tag="JN331_000864"
     CDS             25410..26261
                     /gene="tsf"
                     /locus_tag="JN331_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY7259077.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26408..27133
                     /gene="pyrH"
                     /locus_tag="JN331_000865"
     CDS             26408..27133
                     /gene="pyrH"
                     /locus_tag="JN331_000865"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY7259078.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27425..27982
                     /gene="frr"
                     /locus_tag="JN331_000866"
     CDS             27425..27982
                     /gene="frr"
                     /locus_tag="JN331_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY7259079.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28074..29270
                     /gene="ispC"
                     /locus_tag="JN331_000867"
     CDS             28074..29270
                     /gene="ispC"
                     /locus_tag="JN331_000867"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY7259080.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            29459..30217
                     /gene="ispU"
                     /locus_tag="JN331_000868"
                     /gene_synonym="uppS"
     CDS             29459..30217
                     /gene="ispU"
                     /locus_tag="JN331_000868"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY7259081.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30230..31087
                     /gene="cdsA"
                     /locus_tag="JN331_000869"
     CDS             30230..31087
                     /gene="cdsA"
                     /locus_tag="JN331_000869"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY7259082.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31099..32451
                     /gene="rseP"
                     /locus_tag="JN331_000870"
     CDS             31099..32451
                     /gene="rseP"
                     /locus_tag="JN331_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY7259083.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMFVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32481..34913
                     /gene="bamA"
                     /locus_tag="JN331_000871"
     CDS             32481..34913
                     /gene="bamA"
                     /locus_tag="JN331_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY7259084.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35035..35520
                     /gene="skp"
                     /locus_tag="JN331_000872"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35035..35520
                     /gene="skp"
                     /locus_tag="JN331_000872"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY7259085.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35524..36549
                     /gene="lpxD"
                     /locus_tag="JN331_000873"
     CDS             35524..36549
                     /gene="lpxD"
                     /locus_tag="JN331_000873"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY7259086.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36654..37109
                     /gene="fabZ"
                     /locus_tag="JN331_000874"
     CDS             36654..37109
                     /gene="fabZ"
                     /locus_tag="JN331_000874"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY7259087.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37113..37901
                     /gene="lpxA"
                     /locus_tag="JN331_000875"
     CDS             37113..37901
                     /gene="lpxA"
                     /locus_tag="JN331_000875"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY7259088.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37901..39049
                     /gene="lpxB"
                     /locus_tag="JN331_000876"
     CDS             37901..39049
                     /gene="lpxB"
                     /locus_tag="JN331_000876"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY7259089.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39046..39642
                     /gene="rnhB"
                     /locus_tag="JN331_000877"
     CDS             39046..39642
                     /gene="rnhB"
                     /locus_tag="JN331_000877"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY7259090.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            39679..>42007
                     /gene="dnaE"
                     /locus_tag="JN331_000878"
     CDS             39679..>42007
                     /gene="dnaE"
                     /locus_tag="JN331_000878"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY7259091.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLTFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKA"
BASE COUNT         9873 a        10289 c        11664 g        10181 t
ORIGIN      
        1 ccctctccct caagggagag gggaccgatc gagcacaaat tttgagagat atcttcttct
       61 gtcttgtaac agaagaacag aaaatcgggc ttttgcgcct gaatattgcg gagaaaaagc
      121 atggccggga atgaccgcga gccaattgga cgcaaaggga aaccgacgcg tccggtcaaa
      181 caaaaggtaa gccgtcgtcg ttacgaagat gacgatgatt acgacgatta tgatgactat
      241 gaggatgaag aaccgatgcc gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt
      301 ggcaaacgcg gctggctatg gctactgcta aaactggcta tcgtttttgc cgtgctgatc
      361 gccatttacg gcgtttatct cgatcaaaaa attcgtagcc gtattgatgg caaggtctgg
      421 caactgcccg cggcagttta tggccgaatg gtcaaccttg aaccagatat gaccatcagc
      481 aagaacgaaa tggtgaagct gctggaggca acacagtatc gtcaggtgtc gaaaatgacc
      541 cgtcctggcg aatttaccgt gcaggccaac agcattgaga tgatccgccg tccgtttgat
      601 ttcccggaca gtaaagaagg gcaggtgcgt gcgcgtctga cctttgatgg tgatcatctg
      661 gcgaccatcg tcaatatgga gaacaaccgt cagttcggtt tcttccgtct tgatccgcgt
      721 ctgatcacca tgatctcctc gccaaacggt gagcagcgtt tgtttgtgcc gcgcagtggt
      781 ttccctgatt tgctggtgga tactttgctg gcgacagaag accgtcattt ttacgagcat
      841 gatggaatca gtctctactc cattgggcgt gcggtgctgg caaacctgac cgcaggtcgc
      901 acggtgcagg gtgcgagtac gctgacgcaa cagctggtga aaaacctgtt cctctccagc
      961 gagcgttctt actggcgtaa agcgaacgaa gcttacatgg cgctgatcat ggacgcgcgt
     1021 tacagcaaag accgtattct tgagctgtat atgaacgagg tgtatctcgg tcagagcggc
     1081 gacaacgaaa tccgcggctt cccgctggca agcttgtatt actttggccg cccggtagaa
     1141 gagctaagcc tcgaccagca ggcgctgtta gtcggtatgg tgaaaggggc gtccatctac
     1201 aacccgtggc gtaacccgaa actggcgctg gagcgacgta atctggtgct gcgtctgctg
     1261 caacagcaac agattattga tcaagaactc tatgacatgt tgagtgcccg tccgctgggg
     1321 gttcagccgc gtggtggggt gatctctcct cagccagcct ttatgcagct ggtgcgtcag
     1381 gagttgcagg caaaactggg cgataaggta aaagatctct ccggcgtgaa gatcttcact
     1441 acctttgact cggtggccca ggacgcggca gaaaaagccg ccgtggaagg cattccggca
     1501 ctgaagaaac agcgtaagtt gagcgatctt gaaactgcga ttgtggtcgt cgaccgcttt
     1561 agtggtgaag ttcgtgcgat ggtcggaggt tctgagccgc agtttgcggg ctacaaccgt
     1621 gcgatgcagg cgcgtcgttc gattggttcc cttgcaaaac cagcgactta tctgacggct
     1681 ttaagccagc cgaaaatcta tcgcctgaac acgtggattg ccgatgcgcc aatcgcgctg
     1741 cgtcagccga atggccaggt ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc
     1801 ggcagagtga tgctggtgga tgcgttgaca cgttcgatga acgtgccgac ggtaaatctg
     1861 gggatggcgc tggggctgcc tgcggttacg gagacctgga tcaaactggg cgtaccgaaa
     1921 gatcagttgc atccggttcc ggcaatgctg ctgggggcgt tgaacttaac gccaatcgaa
     1981 gtggcgcagg cattccagac catcgccagc ggtggtaacc gtgcaccgct ttctgcactg
     2041 cgttcggtaa tcgcggaaga tggcaaggtg ctgtatcaga gcttcccgca ggcagaacgc
     2101 gctgttccgg cgcaggcggc gtatctgaca ctatggacca tgcagcaggt ggtacaacgt
     2161 ggtacgggtc gtcagcttgg ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg
     2221 actaccaaca ataacgtaga tacctggttt gcaggcattg acggcagcac ggtgaccatc
     2281 acctgggtag gccgtgataa caaccagccg accaaactgt atggagccag cggggcaatg
     2341 tcgatttatc agcgttatct ggctaaccag acgccaacgc cgctgaatct tgttccgcca
     2401 gaagatattg cagatatggg cgtggactac gacggcaact ttgtttgcag cggtggcatg
     2461 cgtgtcttgc cggtctggac cagcgatccg caatcgctgt gccagcagag cgagatgcag
     2521 cagcagccgt caggcaatcc gtttgatcag tcttctcagc cgcagcaaca gccgcaacag
     2581 caacctgctc agcaagagca gaaagacagc gacggtgtag ccggttggat caaggatatg
     2641 tttggtagta attaacatct aagcgtgaaa taccggatgg cgagttgcca tccggtaaaa
     2701 taacatccca tctaagatat taaccctttc ttttcatctg gttgtttatt aacccttcag
     2761 gaacgctcag attgcgtacc gcttgcgaac ccgccagcgt ttcgaatatt atcttatctt
     2821 tataataatc attctcgttt acgttatcat tcactttaca tcagagatat accaatggcg
     2881 cgttccaaaa ctgctcagcc aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca
     2941 gcggttagcg gcatgtctgt ttatgcacag gcagcggttg aaccgaaaga agacactatc
     3001 accgttaccg ctgcacctgc gccgcaagaa agcgcatggg ggcctgctgc aactattgcg
     3061 gcgcgacagt cagctaccgg cactaaaacc gatacgccga ttcaaaaagt gccacagtct
     3121 atttctgttg tgaccgccga agagatggcg ctgcatcagc cgaagtcggt aaaagaagcg
     3181 cttagctaca cgccgggtgt ctctgttggt acgcgtggcg catctaacac ctatgaccac
     3241 ctgatcattc gcggttttgc ggcagaaggc caaagccaaa ataactatct gaatggcctg
     3301 aagttgcagg gcaacttcta taacgatgcg gtcattgacc cgtatatgct ggaacgcgct
     3361 gaaattatgc gtggcccggt ttccgtgctt tacggtaaaa gcagtcctgg cggtctgttg
     3421 aatatggtca gcaagcgtcc gaccaccgaa ccgctgaaag aagttcagtt taaagccggt
     3481 actgacagcc tgttccagac tggttttgac tttagcgatg cgttggatga tgacggtgtt
     3541 tactcttatc gcctgaccgg tcttgcgcgt tctgccaatg cccagcagaa agggtcagaa
     3601 gagcagcgtt atgctattgc accggcgttc acctggcgtc cggatgataa aaccaatttc
     3661 accttccttt cttacttcca gaacgagccg gaaaccggtt attacggctg gttgccgaaa
     3721 gagggaaccg ttgagccgct gccgaacggt aagcgtctgc cgacagactt taatgaaggg
     3781 gcgaagaaca acacctattc tcgtaatgag aagatggtcg gctacagctt cgatcacgaa
     3841 tttaacgaca cctttactgt gcgtcagaac ctgcgctttg ctgaaaacaa aacctcgcaa
     3901 aacagcgttt atggttacgg cgtctgctcc gatccggcga atgcttacag caaacagtgt
     3961 gcggcattag cgccagcgga taaaggccat tatctggcac gtaaatacgt cgttgatgat
     4021 gagaagctgc aaaacttctc cgttgatacc cagttgcaga gcaagtttgc cactggcgat
     4081 atcgaccaca ccctgctgac cggtgtcgac tttatgcgta tgcgtaatga catcaacgcc
     4141 tggtttggtt acgacgactc tgtgccactg ctcaatctgt acaatccggt gaataccgat
     4201 ttcgacttca atgccaaaga tccggcaaac tccggccctt accgcattct gaataaacag
     4261 aaacaaacgg gcgtttatgt tcaggatcag gcgcagtggg ataaagtgct ggtcacccta
     4321 ggcggtcgtt atgactgggc agatcaagaa tctcttaacc gcgttgctgg gacgaccgat
     4381 aaacgtgatg acaaacagtt tacctggcgt ggtggtgtta actacctgtt tgataatggt
     4441 gtaacacctt acttcagcta tagcgaatcg tttgaacctt cttcgcaagt tgggaagaat
     4501 ggtaatattt tcgcaccgtc taaaggtaag cagtatgaag tcggcgtgaa atatgtaccg
     4561 gaagatcgtc cgattgtagt tactggtgcc gtgtataatc tcactaaaac caacaacctg
     4621 atggcggacc ctgagggttc cttcttctcg gttgaaggtg gcgagatccg cgcacgtggc
     4681 gtagaaatcg aagcgaaagc ggcgctgtcg gcgagtgtta acgtagtcgg ttcttatact
     4741 tacaccgatg cggaatacac caccgatact acctataaag gcaatacgcc tgcacaggtg
     4801 ccaaaacaca tggcttcgct gtgggctgac tataccttct ttgacggtcc gctttcaggt
     4861 ctgacgctgg gcaccggtgg tcgttatact ggctccagct atggtgatcc ggctaactcc
     4921 tttaaagtgg gaagttatac ggtcgtggat gcgttagtgc gttatgatct ggcgcgagtc
     4981 ggcatggcgg gctccaacgt ggcgctgcat gttaacaacc tgttcgatcg tgaatacgtc
     5041 gccagctgct ttaacactta tggctgcttc tggggcgcag aacgtcaggt cgttgcaacc
     5101 gcaacctttc gtttctaatt tctcttttgg ggcacggatt tccgtgccca tttcacaagt
     5161 tggctgttat gcaggaatac acgaatcatt ccgataccac ttttgcactg cgtaatatct
     5221 cctttcgtgt gcccgggcgc acgcttttgc atccgctgtc gttaaccttt cctgccggga
     5281 aagtgaccgg tctgattggt cacaacggtt ctggtaaatc cactctgctc aaaatgcttg
     5341 gtcgtcatca gcctccgtca gaaggggaga ttcttcttga tgcccagccg ctggaaagct
     5401 ggagcagcaa agcgtttgcc cgcaaagtgg cttatttgcc gcagcagctt cctccggcag
     5461 aagggatgac cgtgcgtgaa ctggtggcga ttggtcgtta cccgtggcat ggcgcgctgg
     5521 ggcgctttgg tgcggcagat cgcgaaaagg tcgaggaagc tatctcgctg gttggcttaa
     5581 aaccgctggc gcatcggctg gtcgatagtc tctctggcgg cgaacgtcag cgggcgtgga
     5641 tcgccatgct ggtggcgcag gatagccgtt gtctgttgct cgacgaaccg acttcggcgc
     5701 tggatatcgc ccaccaggtt gatgtgctgg cgctggtgca ccgtttaagt caggaacgtg
     5761 gcctgacggt cattgccgtg ttgcacgata tcaatatggc ggcacgctac tgtgattatc
     5821 tggtcgccct gcgcagtggt gaaatgattg ctcagggaac gcctgcggaa attatgcgcg
     5881 gcgaaaccct cgaaatgatt tatggcatcc cgatgggtat tttgccgcat ccggcgggtg
     5941 ctgcacctgt gagttttgtt tattgatgag cggcttacct cttatttcgc gccgtcgact
     6001 gttaacggcg atggcgcttt ctccgttgtt atggcagatg aataccgccc acgcggcggc
     6061 tattgacccc aatcgtattg tggcgctgga gtggttgccg gtggaattac tgctggcgct
     6121 cggcatcgtg ccttacggcg tggcggatac catcaactat cgcctgtggg tcagcgaacc
     6181 accattgccg gactcagtga ttgacgtcgg tttgcgtaca gaacctaacc ttgaactgct
     6241 gaccgaaatg aaaccgtcgt ttatggtctg gtcggcagga tatggccctt catcagaaat
     6301 gctggcacgt attgcgccgg gtcgcggatt taacttcagt gacggcaaac atccgttggc
     6361 gatggcgcgt aaatcgctga cggaaatggc agatttactt aacctgcaaa gcgcagcgga
     6421 aacgcattta gcgcaatacg aagactttat ccgcagcatg aaaccccgct ttgtgaagcg
     6481 tggtgcacgc ccgttactgc tgacgacgct tatcgatccg cgccatatgc tggtcttcgg
     6541 tccaaacagc ttgttccagg aaattctcga tgagtacggc atcccaaatg cctggcaagg
     6601 ggaaaccaac ttctggggca gtaccgccgt cagtatcgat cgtctggcag cgtataaaga
     6661 cgttgatgtg ctctgttttg atcacgacaa cagcaaagac atggatgcgc taatggcaac
     6721 cccgctgtgg caggccatgc cgtttgtccg tacagggcgc tttcagcgcg tacccgcagt
     6781 ctggttttat ggtgcaacgc tttcggcaat gcactttgtg cgtgttctgg ataacgccat
     6841 cggaggtaaa gcgtgagtaa acgaattgcg cttttcccgg cgttattgct ggcgctgtta
     6901 gtgattgtcg ctacggcgct cacctggatg aacttctcac aggcgctgcc gcgcagtcag
     6961 tgggcgcagg ccgcctggtc gccggatatt gacgtcatcg agcagatgat ttttcactac
     7021 agcttgttgc cgcgtctggc gatttcgctg ctggtgggcg cgggcctggg gctggtgggc
     7081 gtgctgtttc agcaagtact gcgtaacccg ctggcggaac cgacgacgtt gggcgttgca
     7141 acaggcgcgc aactggggat taccgtcacc acgctctggg cgatccctag cgcgatggcg
     7201 agccagtttg ctgcgctggc aggggcttgc gttgttggct taattgtctt tggcgtcgcg
     7261 tgggggaaac ggctgtcgcc ggtaacgctg attctcgcgg ggttggtagt gagcctttat
     7321 tgcggcgcaa tcaatcagtt actggttatc ttccatcatg accagttgca aagcatgttt
     7381 ctgtggagca ctggaacgct gacgcaaacc gactggggcg gtgttgagcg tttatggccg
     7441 cagttgctgg gcggtgtgat gctgacgttg ctgctacttc gtccgttaac cctgatgggg
     7501 cttgatgatg gcgtggcgcg caatctcggg ctggccttgt cgcttgcgcg tctggcggcg
     7561 ctgtcgctgg cgattgtcat cagtgcgctg ctggtgaacg ctgtggggat tatcggcttt
     7621 atcgggttgt tcgcgccgct gctggcaaaa atgctggggg cgcggcgtct gctgccacga
     7681 ctgatgctgg cgtcgttgat tggtgcgctg atcctctggc tttccgatca aatcatcctc
     7741 tggctgactc gcgtgtggat ggaagtgtcc accggttcgg tcactgcgtt gatcggtgcg
     7801 ccgctgctac tgtggctgct gccgcgttta cgcagcatta gcgcgccgga tatgaaggtt
     7861 aacgatcgtg tcgcgactga acgccaacat gtgctggcgt ttgccctcgc gggcggcgtg
     7921 ctgttgttga tggctgtggt ggtggcgctg tcgtttggtc gtgatgcgca cggctggacg
     7981 tgggcgagcg gggcgttgct cgaggattta atgccctggc gctggccgcg aattatggcg
     8041 gcgctgtttg cgggcgtcat gctggcggta gcgggctgta ttattcagcg actgaccggc
     8101 aacccgatgg caagcccgga agtgctgggg attagctccg gcgcggcgtt tggtgtggtg
     8161 ttaatgctgt ttctggtacc gggtaatgcc tttggctggc tgttacccgc aggcagtctc
     8221 ggggcggcgg tgacgctgtt gatcattatg atcgccgccg gacgcggtgg attttcccca
     8281 caccgcatgt tactggcggg gatggcgtta agtaccgcgt tcaccatgct tttgatgatg
     8341 ttgcaggcaa gtggtgaccc gcgaatggcg caagtgctga cctggatttc cggttctacc
     8401 tacaacgcga ccgatgctca ggtctggcgc accggaattg tgatggtgat tttgctggcg
     8461 attactccgc tgtgccgccg ctggctgacc attttaccgc tgggtggtga taccgcgcgg
     8521 gcggtaggga tggcgctgac gccgacgcga attgcgctgc tgctgttagc ggcttgcctg
     8581 acggcgaccg cgacgatgac tattggaccg ttgagttttg ttggtttaat ggcaccgcat
     8641 attgcgcgga tgatgggctt tcgacggacg atgccacaca tcgtaatttc ggcgctggtg
     8701 ggtggtttac tgctggtgtt cgctgactgg tgtgggcgga tggtgctgtt tccattccag
     8761 atcccggcgg ggctgctgtc gacctttatc ggtgcgccat attttatcta tttgctgaga
     8821 aagcagagcc gttaatagaa tgcctcgccc ggtgggcgag gcaaaattat cacaacttcg
     8881 cgaacacccg acgtgcagca tcgatggtgt tattgatatc ttccatgctg tgcgccacag
     8941 acataaagcc cgcttcaaac gctgacggtg ccaggtaaac accttcatcc agcatcatat
     9001 ggaagaaacg cttaaaacgc tcgacgtcac aggccatcac atcctgatag cacgtcacgg
     9061 actcggcgtc ggtaaagaag ataccgaaca taccaccgac gtggttaacg accagcggaa
     9121 ttcccgattc ttctgccgct tccagcagac cttctgccag acgtgttgtc agctcatcca
     9181 gcgtttcgtg aatgcccggc tgcgcgactt cattcagaca ggcgaaaccc gctgccatcg
     9241 caatcgggtt accggaaagc gtacccgcct gatagaccgg acctgtcggg gccagcgcat
     9301 ccattacatc acgacgacca ccgaatgcgc ctaccggcat tccaccgccg atgatttttc
     9361 cgaggcaggt taaatctggc actacgccga aataatcctg tgcgccagct agcgctacgc
     9421 ggaaaccggt catcacttca tcgatgatca gcaacgcgcc aaattcgtcg cacagcgcac
     9481 gcagacctgg caggaactct ggcagcggcg gaacacagtt catattgcct gccaccggct
     9541 cgacgataat acaggcaatc tcttgcgggt attgctcaaa cgcggcgcgt acagaggcca
     9601 gatcgttata agtacaggtt aaggtatgtt tggcgaaatc tgccggaacg cccggcgagt
     9661 ttggctggcc taacgtgagt gcgccagaac cggctttcac cagcaggcag tcagcgtgac
     9721 cgtggtaaca accttcaaat ttaatgattt tgtcgcgacc ggtaaaacca cgggccaggc
     9781 ggatggcgct catcgtcgcc tcggtgccgg agttcaccat gcgcaccata tccatggtcg
     9841 ggaccagttc ggtcaccagt tgcgccattt tcacttccat ttcggtcggt gcaccaaagc
     9901 ttaaaccacg ctcggcggct tcaatcacgg cattgcggat cgccggatgg ttatggccca
     9961 gcaccatcgg cccccaggaa ccgacataat cgatataggc tttgccatca acatcgtaca
    10021 gataagcgcc gtccgctttt tcgataaaca gtggagtgcc gcccacgcca gtaaaggcgc
    10081 gaacagggga gttcacaccg ccagggatca gctcgcgcgc tgcgctgtaa agattttcag
    10141 acttactcat ggagggttcc tgattcgtag aaaaagtgaa tggctgctat tctatgttat
    10201 tcataacaag ttaaatactc gtcaaacatc aggctgcttg tactggtgca atcctgattt
    10261 cttagagtat aaaagttttg tgcatttgaa acattacgct ttgcaaagga ttttcatgga
    10321 acgtgcgagt aaaatgccgt catcttattt gtatgaccaa taagtgatca ttggatgaaa
    10381 actgatactc cctctttaga aacaccgcag gccgcgcgcc tgcgacgcag acaactgatt
    10441 cgccaacttc ttgagcgcga taaaaccccg ttagccattt tgtttatggc ggcagtcgtc
    10501 ggcacgcttg tcgggctggc tgcggttgct tttgacaaag gtgtcgcctg gttgcagaac
    10561 caacgtatgg gggcgctggt acatactgct gataattatc cgctactgtt aaccgtcgct
    10621 tttctctgtt cggcggtgct ggcgatgttc ggctactttt tggtgcgcaa atacgcgccc
    10681 gaagcagggg gatcggggat cccggaaatt gaaggggcgc tggaagatca acgtcctgtt
    10741 cgctggtggc gtgtattgcc ggtgaaattc tttggcgggc tggggacact cggcggaggc
    10801 atggtgctag ggcgcgaagg accaaccgtg cagatcggcg gcaatatcgg tcgcatggtg
    10861 cttgatattt tccgcctgaa aggtgatgaa gctcgccata cgctgctggc aaccggtgct
    10921 gctgcggggc tggctgcggc ctttaacgcg ccgctggcgg gtattttgtt tattatcgaa
    10981 gagatgcgtc cgcagtttcg ctatacgtta atttcgatta aagcggtatt tattggcgtc
    11041 attatgtcga ccattatgta ccggattttt aatcatgaag ttgcgttgat tgacgtcggt
    11101 aaactttctg atgcgccgct taatacgctg tggctttatc tgatcctcgg tattattttt
    11161 ggcattttcg gccctatttt taataaatgg gtgctgggga tgcaggattt gctgcaccgt
    11221 gtgcacggcg gcaatattac caaatgggta ctgatggggg gtgcgattgg cggtctgtgt
    11281 ggattgctgg ggtttgtggc accagcaacg tcgggcggcg gttttaacct gattcctatc
    11341 gctaccgcgg ggaatttcag catgggaatg ctggtgttta tcttcgtcgc tcgggtcatt
    11401 accaccttac tctgcttctc ttccggcgcg ccgggcggta tttttgcccc gatgctggcg
    11461 ctgggtactg tgctgggaac cgctttcgga atggttgccg ttgagctgtt tccgcaatat
    11521 cacctggagg cggggacgtt tgctattgcc ggaatggggg cattactggc ggcatctatt
    11581 cgcgcgccgt taacggggat cattctggtt ctggagatga ccgataacta ccagctcatt
    11641 ttgccaatga ttattaccgg tcttggcgca acactattag cgcaatttac cggcgggaaa
    11701 cctctatact cggcgattct tgcgcgcacg ctggcaaaac aggaagctga gcaactggcg
    11761 cgaagcaagg ccgcatcagc cagcgagaat acttgaacga aataccaggg tattagataa
    11821 tggcgattat tattgggtta gaatttgccc gattgccgat gtcgtttgga gcaaaatatg
    11881 agtgatgacg tagcactgcc gctggagttt accgacgcag cagccaacaa agttaaaagc
    11941 ctgatcgctg acgaagataa cccgaatctg aaattacgcg tgtatatcac cggtggcggt
    12001 tgcagcggct tccagtatgg tttcaccttt gatgatcagg tgaacgaagg cgatatgacc
    12061 atcgaaaaac agggcgttgg cctggtggtt gatccgatga gcctgcaata tctggtcggc
    12121 ggttccgttg actacaccga aggactggaa ggttctcgtt tcatcgtgac caacccgaac
    12181 gcgaaaagca cctgcggttg cggttcttcc tttagcatct aatctgttgt tcatgtgccg
    12241 gataaactat ccggcacatc ttctctcttc aacgcccatt ctcatccagc gcaaacgtcg
    12301 gtagcttaag atgccaacga atagccgcca gccgaatcaa tagcgtcacg accatgccca
    12361 tcatactggc tgtttccagt ggtacggaaa atgtgtaata agccgtagcg tggacaatac
    12421 cgccgataat acaggcagtt gcgtagattt ctgtacgtaa aatcatgggg atttcgcggg
    12481 ccagtacatc acgaatgatc ccgccgccaa cgccagtaat gacgcccata caaaccgcga
    12541 ttaacggacc ggcttccgca ttaaaggctt tattcacgcc aatgccgaca aacaccgcca
    12601 gaccaacggc gtccagcacc ggcaacatcc attttggtaa gcgtcttggc tggcgcacca
    12661 gcacgatggt cagcatgctg gtgaccatgg caacgaccag atcggtggga tctttcaccc
    12721 aaaataccgg gccgtgatcc agcgccatgt cgcgaattgt tccgtcgcct actgcggtaa
    12781 ccacgcccag taccagaaca ccaaaagggt ccatacgcaa ttttccggct aacaaaacgc
    12841 cggagatggc aaataccgct gtgccgacta tatccagcca atagacgagc attgttcaat
    12901 ccccactgag cacctgaaaa ggtcaggcac taatctacct gtgaaagcgc attacagagc
    12961 tgttgtgctg cgaggataat acgtgggctt gcacgttcaa accagtcact cgtgagagga
    13021 ataacgggaa ttttgagctg ttcaccccag tattgtttga ttttaggaat ttggtccggt
    13081 ccgcctgtaa tgacaatcgc ctgtggcgag cgtgctaaca cctgttcgcg gctaacttgc
    13141 ggccagggaa cccggctgtc tttaaagatg ttttctccgc cacaaacttc gagtacctgg
    13201 ttctgaatcg actcttttcc actggtaaat ggcggattaa tgccgaattg cagaaaaaca
    13261 cgttttttag gtttatcagc atattgcgct ttcaattgca cgtactgatc cagcagggat
    13321 tgcgcggctt gttcggcctt gtctggttgc ggactccagg gggccagttg acgtaacgca
    13381 ttggcaattt gttcaatgct tgtcgcatcg acccacatca cttttattcc cagcgaagcc
    13441 agctggtcaa cctgccgctc ggcattacct ccacgccagg caatcaccag atcgggtttc
    13501 agcgcgacaa tgcgttccag attcatcccc tgccaggtgg aaacctgctc aatcttttgc
    13561 gcttgtggag gatagtcgga atagctgctg accccaaccg gcgtgatccc ggcggcaaag
    13621 gcaagttcag tgttggcggg agaaagcgtg atgacgcgcg gcgcggcgtt gagccacagt
    13681 ggcgcgagaa aagacagggc gaccagcgcc ctgaacagtg atttagccat gtgccagttt
    13741 ctgcaccagt gactcaacca tcaggctgga ctgtttagcg gcaacagcca ggaactcatc
    13801 gaagctaaga tgagactgtt gatcggccac gtcggagatg gcgcgtacga caacaaacgg
    13861 gatgttgaaa ttgtggcaga catgggcgat tgccgtcgct tccatctcta cagcaatggc
    13921 ctgtgggaag ttgtggcgga ttttcgccag accaacagaa ccgttgatga aagcgtcgcc
    13981 gctaacaatc aggccacgta cagcgttaag attcagttcg gcaatgcagg cctcagcggc
    14041 agcgatcagt ttatcgtcag ctttaaagcc tgccggacag cctggtaact gaccgtattc
    14101 ataaccaaat gccgtgacat ccgcgtcgtg ataacgcgct tcgtccgaga caacgatatc
    14161 gcccactttc agcgttggtg ccaggcaacc ggcagaaccg gtgttaataa tcacatctgg
    14221 cttgcagtgt tccaacagca gagtggcacc cagcgccgca gcgactttac cgatgcccga
    14281 tttcagaagc gcaacctcgg ttccattcag ttggccggta tagatttcgc aaccgccgag
    14341 actgatagtt tgacggtttt cgattttgtc acgcagcagc gtaacttctt cttccattgc
    14401 accaatgatg ccgattttca tagatttact cgcgataagc ccgatttgaa ggcatagttt
    14461 accatgcgct tacggggaag cgtatttctc acgcgggaga ggacatggca cagattgatt
    14521 tccgaaaaaa aataaactgg catcgtcgtt accgttcacc gcagggcgtt aaaaccgaac
    14581 atgagatcct gcggatcttc gagagcgatc gcgggcgtat catcaactct ccggcaattc
    14641 gtcgtctgca acaaaagacc caggtttttc cactggagcg caatgccgcc gtgcgcacgc
    14701 gtcttaccca ctcgatggaa gtccagcagg tgggacgcta catcgccaaa gaaattttaa
    14761 gccgtctgaa agagcttaaa ttgctggaag catacggcct ggatgaactg accggcccct
    14821 ttgaaagcat tgttgagatg tcatgcctga tgcacgatat cggcaatccg ccgtttggtc
    14881 attttggcga agcagcgata aatgactggt ttcgccaacg tttgcacccg gaagatgccg
    14941 aaagccagcc tctgactgac gatcgctgca gcgtggcggc actacgttta cgggacgggg
    15001 aagaaccgct taacgagctg cggcgcaaga ttcgtcagga cttatgtcat tttgagggga
    15061 atgcacaagg cattcgtctg gtgcatacat tgatgcggat aaatctcact tgggcacagg
    15121 ttggcggtat tttaaaatat acccgtccgg cgtggtggcg tggcgaaacg cctgagacac
    15181 atcactattt aatgaaaaag ccgggttatt atctttctga agaagcctat attgcccggt
    15241 tgcgtaaaga acttaatttg gcgctttaca gtcgttttcc attaacgtgg attatggaag
    15301 ctgccgacga catctcctat tgtgtggcag accttgaaga tgcggtagag aaaagaatat
    15361 ttaccgttga gcagctttat catcatttgc acgaagcgtg gggccagcat gagaaaggtt
    15421 cgctcttttc gctggtggtt gaaaatgcct gggaaaaatc acgctcaaat agtttaagcc
    15481 gcagtacgga agatcagttt tttatgtatt tacgggtaaa caccctaaat aaactggtac
    15541 cctacgcggc acaacgattt attgataatc tgcctgcgat tttcgccgga acgtttaatc
    15601 atgcattatt ggaagatgcc agcgaatgca gcgatcttct taagctatat aaaaatgtcg
    15661 ctgtaaaaca tgtgtttagc catccggatg tcgagcagct tgaattgcag ggctatcggg
    15721 tcattagcgg attattagag atttatcgtc ctttattaag cctgtcgtta tcagacttta
    15781 ctgaactggt agaaaaagaa cgggtgaaac gtttccctat tgagtcgcgc ttattccaca
    15841 aactctcgac gcgccatcgg ctggcctatg tcgaggctgt cagtaaatta ccgtcagatt
    15901 ctcctgagtt tccgctatgg gaatattatt accgttgccg cctgctgcag gattatatca
    15961 gcggtatgac cgacctctat gcgtgggatg aataccgacg tctgatggcc gtagaacaat
    16021 aaccaggctt ttgtaaagac gaacaataaa tttttacctt ttgcagaaac tttagttcgg
    16081 aacttcgggc tataaaacga atctgaagaa cacagcaatt ttgcgttatc tgttaatcga
    16141 gactgaaata catgaaaaaa accacattag cactgagtgc actggctctg agtttaggtt
    16201 tggcgttatc tccgctctct gcaacggcgg ctgagacttc ttcagcaacg acagcccagc
    16261 agatgccaag ccttgcaccg atgctcgaaa aggtgatgcc ttcagtggtc agcattaacg
    16321 tagaaggtag cacaaccgtt aatacgccgc gtatgccgcg taatttccag cagttcttcg
    16381 gtgatgattc tccgttctgc caggaaggtt ctccgttcca gagctctccg ttctgccagg
    16441 ggggccaggg cggtaatggc ggcggccagc aacagaaatt catggcgctg ggttccggcg
    16501 tcatcattga tgccgataaa ggctatgtcg tcaccaacaa ccacgttgtt gataacgcga
    16561 cggtgattaa agtccagctg agcgatggtc gtaagttcga cgcgaagatg gttggcaaag
    16621 atccgcgctc tgatatcgcg ctgatccaaa tccagaaccc gaaaaacctg accgcaatta
    16681 agatggcgga ttctgatgca ctgcgcgtgg gtgattacac cgtagcgatt ggtaacccgt
    16741 ttggtctggg cgagacggta acttccggga ttgtctctgc gctggggcgt agcggcctga
    16801 atgctgaaaa ctacgaaaac ttcatccaga ccgatgcagc gatcaaccgt ggtaactccg
    16861 gtggtgcgct ggttaacctg aacggcgaac tgatcggtat caacaccgcg atcctcgcac
    16921 cggacggcgg caacatcggt atcggttttg ctatcccgag taacatggtg aaaaacctga
    16981 cctcgcagat ggtggaatac ggccaggtga aacgcggtga gctgggtatt atggggactg
    17041 agctgaattc cgaactggcg aaagcgatga aagttgacgc ccagcgcggt gctttcgtaa
    17101 gccaggttct gcctaattcc tccgctgcaa aagcgggcat taaagcgggt gatgtgatca
    17161 cctcactgaa cggtaagccg atcagcagct ttgccgcact gcgtgctcag gtgggtacta
    17221 tgccggtggg cagcaaactg accctgggct tactgcgcga cggtaagcag gttaacgtga
    17281 acctggaact gcagcagagc agccagaatc aggttgattc cagctccatc ttcaacggca
    17341 ttgaaggcgc tgagatgagc aacaaaggca aagatcaggg cgtggtagtg aacaacgtga
    17401 agacgggcac tccggctgcg cagatcggcc tgaagaaagg tgatgtgatt attggtgcga
    17461 accagcaggc agtgaaaaac atcgctgaac tgcgtaaagt tctcgacagc aaaccgtctg
    17521 tgctggcact gaacattcag cgcggcgaca gcaccatcta cctgttaatg cagtaatctc
    17581 cctcaacccc ttcctgaata cgggaagggg ttctccttac aatctgtgaa cttcgccaca
    17641 actccataca tcttcatcat cctttgggca tttgcacaat gccgtacgtt acgtacttcc
    17701 ttatgctaag ccgtgcataa cggaggactt atggctggct ggcatcttga taccaaaatg
    17761 gcgcaggata tcgtggcacg taccatgcgc atcatcgata ccaatatcaa cgtaatggat
    17821 gcccgcgggc gaattatcgg cagcggcgat cgtgagcgta ttggtgaatt gcacgaaggt
    17881 gcattgctgg tactttcaca gggacgagtc gtcgatatcg atgacgcggt agcacgtcat
    17941 ctgcacggtg tgcggcaggg gattaatcta ccgttacggc tggaaggtga aattgtcggc
    18001 gtaattggcc tgacaggtga accagagaat ctgcgtaaat atggcgaact ggtctgcatg
    18061 acggctgaaa tgatgctgga acagtcgcgg ttgatgcact tgttggcgca ggatagccgt
    18121 ttgcgggaag aactggtgat gaacctgatt caggcagagg agaatactcc cgcacttctg
    18181 aatgggcgca acggctgggg atcgatctca atcaaccgcg agtggtggct attgttgagg
    18241 tcgacagcgg tcagcttggc gtggacagcg caatggcgga gttacaacaa ctgcaaaacg
    18301 cgctgactac gcccgagcgt aataatctgg tggcgattgt ctcgctaacc gaaatggtgg
    18361 tgttgaaacc ggcgttgaac tcttttgggc gctgggatgc agaagatcat cgtaagcgag
    18421 ttgaacaact gattacccgc atgaaagagt acggccagct gcgttttcgc gtttcactgg
    18481 gcaactattt taccggtcct ggcagtattg cccgatccta tcgtacggcg aaaacgacga
    18541 tggtggtggg taaacagcgg atgccagaaa gtcgctgcta tttttatcag gatctgatgt
    18601 tacctgtgtt actcgacagt ttgcgtggcg actggcaggc caacgaactg gcgcgaccgc
    18661 tggcgcggct gaaagcgatg gacaataacg gcttgctgcg acgaacgctg gcggcgtggt
    18721 ttcgccacaa tgtgcaaccg ctggcaacgt caaaggcgtt gtttattcat cgtaataccc
    18781 tggagtatcg gcttaatcgt atatcggaac tgaccgggct tgatttgggc aattttgatg
    18841 acaggttgct gctgtatgtg gcgttacaac tggatgaaga gcggtagatt atgcgttaag
    18901 gttgaggcgg cgctggctca ttcccctcag cccgaagggg agaggggaat gcccgagtta
    18961 accgtcaatt cgggattatt tacgcgttag tttttccaga tcggcttcaa tctcgctgat
    19021 cttattggtt acgactgact ccaggtgacg taagtcgtcg aggatcttac gcttaagatc
    19081 aacttcgctg cggtcacgct gacagatttg atcaagctca tcaatgatat accgtagatt
    19141 cgggctgatt tcctggactt ctttataacc ctgacccaca ccatcagcga cgaccgtttt
    19201 acgctgacgc ggatatttaa acttaacgct cttggcgaaa aactcgcctt tgtctttctg
    19261 gaaatagatt ttcagaatat cgttgttggc ttcctgccgg aggctgtaac gatcaatttc
    19321 ttcaggattg gtaataccca gacttttcag attgtcgtac atagcgttac ctcaaaatga
    19381 gtcagtaaat tgtgcttatt ttagcatttg gcttggcccg cccggctcga ctgtgatcgc
    19441 agaaagccgt tttcttccag acttctatca tggcgtaaaa atcaaaaaat tacctgcttt
    19501 attgtggcga tattccatgg gatggattag cacatttatc ttcgttttgc gaattacatg
    19561 acagtccgaa acaagatgaa aaagtattta aaaaaggggg aggaagaatg agtaggacga
    19621 attattctga gccagaaagc atacgggcag catcatgctg cccgtatatg tactcttaat
    19681 cgatggtacg cagcagttcg ttaatgccga ctttgccgcg agttttcgcg tcaactttct
    19741 taacgataac cgcacagtag aggctgtatt tgccatcttt tgacggcaga ttacctgaaa
    19801 caaccacaga ccccgccgga acgcgaccgt agtggatttc gccggtttca cggtcgtaaa
    19861 tacgggtgct ctgaccaatg tatacgccca tagagatgac ggaaccttct tcgacaatca
    19921 ccccttcaac cacttcagag cgtgcgccaa tgaagcagtt atcttcaata atggtcgggt
    19981 tagcctgcag cggctccagc acgccgccga tgcccacgcc accggaaagg tggacgtttt
    20041 taccaatctg cgcacaagaa ccgacggtcg cccaggtatc aaccatggtg ccttcatcaa
    20101 catatgcgcc gatgttaacg taagacggca tcagcacggt gttacgggca ataaacgcac
    20161 cctgacgtac cgccgctggt ggcacaacgc ggaagccttc tttctggaaa cgtgcttcgt
    20221 cgtagtcggc gaatttcatc ggcactttgt cgaagtagcg gctttctgcc ccttcgatca
    20281 cctgattatc attaatacgg aaagagagca gcaccgcttt tttcaaccac tgatgcgtca
    20341 cccactgacc gtcaattttt tccgctacac gcagtgcgcc ggaatccagc agggcgatca
    20401 cctgatttac cgcttcgcgg gtaacggtgt ctgcattggc tggcgtgatc tcggcacggc
    20461 gttcaaaagc ggtttcaata atgttctgta actgctgcat tgttaaactc ttttcatatc
    20521 agtaaacaca tcacccttta tcgtttggat tgagggcctc tgtcaaccgc tgatgcactt
    20581 cctgctgcaa ctcgttatta agcgcacgcc ggtcagcggt ggcaattatg aataaatctt
    20641 ctactcgctc gccaatggtt gtaattcggg caccatgaag cgaaattccc agatcggcaa
    20701 aaattttccc gactcgcgcc agcagtccag gttggtcgag ggcgatcagt tcgaggaacg
    20761 atttgcggtc ggtatgggtc ggcaaaaacg ttacttcggt ttcaacagta aaatggcgta
    20821 atttggcggg ttggcgacgg ggctgcggtg gctgccagct actttgcgtc agcacctgct
    20881 ccagaccaaa ccgaataacc tcatggcgat ctgcggacag cgggctgcca tcgggttcca
    20941 gcacgataaa ggtatccatc gccataccgt cgcgagtggt gaaaatttgt gcgtcgtgaa
    21001 cacttaaatt gcggcggtct aattcggcac agacggcggc aaacagatag gggcggtccg
    21061 ggctccagat aaaaatctcg gtgcctccac gcgtagcctg cgggctaagc aataccagcg
    21121 gtttgcttaa atcatgctgt aataaatggc gggcatgcca ggccagttga tttgggctat
    21181 ggcggacaaa atagttagca cgacagcgtg accaaatttg gtgcagcgcc tcttcgtcga
    21241 tgttatccat gcgcagtagt gccagtgcct ggagttggtg atggcgaacc cgttcgcgca
    21301 tatccggcgt gttttgcatt ccgcgtcgta gctgcttttc ggtggcaaaa tagagttcgc
    21361 gcaacagact ttgcttccag ctattccaca gcgtttcgtt ggtggcgcaa atgtcagcca
    21421 ctgtcaggca taccagatag cgcagacgat tttccgtttg aacttcttcg gcaaactgct
    21481 tgatgacttc agggtcctga atatcgcggc gttgtgctgt caccgacatc agcaagtgct
    21541 ggcgaaccag ccaggcaacc agctgtgttt cgcgtgagtt cagcccgtgg agttctgcaa
    21601 aatgcactac atcctgagca ccgagaatgg agtggtcgcc gccgcgtcct ttggcgatat
    21661 cgtgaaacag cgcggcgatg aaaatcagct cagttgacgg caggcgcggc cagacgtcca
    21721 cacacaacgg atggcgctgg cgcgtttctt cactggcaaa actctccagt ttcagcatca
    21781 cgcggatagt atgttcatcc accgtgtagg cgtggaacag atcaaactgc atctgcccga
    21841 cgatatgcga ccattgcggc atatacgcgc cgagcacgct atggcgatgc attggcaata
    21901 gcccgcgccg caccgctccg gggtgacgca gaatgctcaa aaacagtttt cgtgcttgcg
    21961 gaatattaca cagcggttgt tgcagatggc ggcgggcatg gcgtaactgg cgcagcgtgg
    22021 tggagtaaat gccggtgatc gcactgttgc gcaccatggt gtagaacata cgcaagatgg
    22081 cttccggctg gcgcataaat agtgtttcat cacgcaggtc gattagcgta ccgcgtagct
    22141 gaaactcatc gtcgagtgga cgtggttttt cgtcggcggg aagggcgagg atggcttcat
    22201 cgaacagttg cagcagcatc tggttgagtt cactgacgcg gcgtgtaacg cggaagtaat
    22261 ccttcatcat ccgctcgacc ggttcgttgc cttcaccact gtaattcaga cgctgagcga
    22321 cgctaagttg gcgatcgaat aacagacgat tatcgtaacg gctgacgacc agatgcaggg
    22381 caaagcgaat acgccacagt atatgcagac attcgtttaa ttccgcccgc tccgctgagg
    22441 ttaagaagcc aaacccgacc atttcatcca gcgatgttgc gccaaaatga cggcgggcca
    22501 cccattgcag agtgtgaata tcgcgcaagc cgccagggct acttttgatg tctggttcaa
    22561 ggttgtagct ggtgccatgg taacgctgat ggcgctggtt ctgttcttca actttcgctg
    22621 cgtagaactt gtcggaaggc cagaatcctt cgctaaaaat atgtttttgc agttcgagga
    22681 agagcgcaac atcgccaatt aataagcggg attcgattaa attggtggcg acggttaaat
    22741 ccgataaccc ttccagcatg cactcttcaa gcgtgcgcac actatgaccg acttccagct
    22801 ttacatccca gagcagcgtt aacagctcgc ccactttttg cgcctgatcg tccgggagct
    22861 ttttacggct taaaatcagt aaatcgatgt cagaaagtgg atgcagctcg ccacgaccgt
    22921 agccaccgac ggcaaccaat gccaggtcgg caatctggct gaatcccgct tcaatccata
    22981 atcgttgcag gagctggtct atgaactcgg tacgtgcctc aatcaactgt tctgcagaga
    23041 tcccattgtc aaaggcatca cccagccaac gctggaaagt atcgatatgg gcttttatcc
    23101 cacagacggt taattcatca cggggccagg cgcatggatt ttgcggttga ccgggcaggg
    23161 tggggagagc ggtgtttgcg tactgttctg gaagggtatt cattgtgcgc cacccataag
    23221 attaaattat cgcattaaaa aagccggcat tcgccggctt catcttattc gtcgtgcgag
    23281 attatcgccg ggatggtgtc atccttgcgt agcgtcaaaa tttcgcagcc gttatcagtc
    23341 accacaatag tatgctcata ttgtgcagac aagctgcgat ctttggtttt taccgtccag
    23401 ccatctttca tggtgcggat ctctttttta cccgcgttga ccattggctc aatggtgaac
    23461 gtcatccccg gtttcagcac gacgttggtt tcacgggagt catagtgcag cacctgcggt
    23521 tcttcatgga agccgcgacc aataccgtgt ccgcaatatt cacgaacgac ggagaagcct
    23581 tctgcttcga caaatttctg aatcgccgca ccgatttcgc gcagattaat gcctggtttt
    23641 accatgcgta gcgccaggta caggctttct tgcgtgatgc ggcacaggcg ttcgcccatg
    23701 atggtcggct taccgacgat aaacattttc gaggtatcgc cgtggaaacc atctttgatt
    23761 acggtgacat caatgttaac gatatcgcca tctttcagca gcttagcatc gtccgggata
    23821 ccgtggcaca ccacttcatt aatagagatg caaacggatt tcggatagcc gtgatagccg
    23881 aggcaggcag aaaccgcgtg ttgttcatta acaatgtaat cattacagat gcgatccagc
    23941 tcgccggtgc tgacgcccgg tttaacatac ggttcgatca tctccagcac ttcggcagcc
    24001 agtcggccag cgacgcgcat tttttcgata tcttctgggg tcttgattga gatagccatt
    24061 aattctgtcc atcagcgtcg gtgataccga caatatatat gtaagtgccg tcaatggtat
    24121 cacacccggg caaattgaga atcattctga atttcgccaa acgtgccact gaaggttttc
    24181 tataatagaa aattcgacgt ctgatgctgt acacagcgcc aacagttatt ggtgtccacg
    24241 acgtatttgt ggtataaagc gcgccggact tccgatccat ttcgtataca cagactggac
    24301 ggaagcgaca atctcacttt gtgtaacaac acacacgtat cggcacatat tccggggtgc
    24361 cctttggggt cggtaatatg ggatacgtgg aggcataacc ccaactttta tatagaggtt
    24421 ttaatcatgg caactgtttc catgcgcgac atgctcaagg ctggtgttca cttcggtcac
    24481 cagacccgtt actggaaccc gaaaatgaag ccgttcatct tcggtgcgcg taacaaagtt
    24541 cacatcatca accttgagaa aactgtaccg atgttcaacg aagctctggc tgaactgaac
    24601 aagattgttt ctcgcaaagg taaaatcctt ttcgttggta ctaaacgcgc tgcaagcgaa
    24661 gcggtgaaag acgctgctct gagctgcgac cagttcttcg tgaaccatcg ctggctgggc
    24721 ggtatgctga ctaactggaa aaccgttcgt cagtccatca aacgtctgaa agacctggaa
    24781 actcagtctc aggacggtac tttcgacaag ctgaccaaga aagaagcgct gatgcgcact
    24841 cgtgagctgg agaaactgga aaacagcctg ggcggtatca aagacatggg cggtctgccg
    24901 gacgctctgt ttgtaatcga tgctgaccac gaacacattg ctatcaaaga agcaaacaac
    24961 ttgggtattc cggtatttgc tatcgttgat accaactctg atccggacgg tgttgacttc
    25021 gttatcccgg gtaacgacga cgcaatccgt gctgtgaccc tgtacctggg cgctgttgct
    25081 gcaaccgtac gtgaaggccg ttctcaggat ctggcttccc aggcggaaga aagcttcgta
    25141 gaagctgagt aataaggctt gataactccc ccaaaatagt tcgagttgca gaaaggcggc
    25201 aagctcgaga attcccggga gcttacatca gtaagtgacc gggatgagcg agcgaagaca
    25261 acgcatctgc ggcgcgaaat atgaaggggg agagccctta tagaccaggt agtacacgtt
    25321 tggttagggg gcctgcatat ggcccccttt ttcactttta tatctgtgcg gtttaatgcc
    25381 gggcagatca catctccgag gattttagaa tggctgaaat taccgcatcc ctggtaaaag
    25441 agctgcgtga gcgtactggc gcaggcatga tggattgcaa aaaagcactg actgaagcta
    25501 acggcgacat cgagctggca atcgaaaaca tgcgtaagtc cggtgctatt aaagcagcga
    25561 aaaaagcagg caacgttgct gctgacggcg tgatcaaaac caaaatcgac ggcaactacg
    25621 gcatcattct ggaagttaac tgccagactg acttcgttgc aaaagacgct ggtttccagg
    25681 cgttcgcaga caaagttctg gacgcagctg ttgctggcaa aatcactgac gttgaagttc
    25741 tgaaagcaca gttcgaagaa gaacgtgttg cgctggtagc gaaaattggt gaaaacatca
    25801 acattcgccg cgttgctgcg ctggaaggcg acgttctggg ttcttatcag cacggtgcgc
    25861 gtatcggcgt tctggttgct gctaaaggcg ctgacgaaga gctggttaaa cacatcgcta
    25921 tgcacgttgc tgcaagcaag ccagaattca tcaaaccaga agacgtatcc gctgaagtgg
    25981 tagaaaaaga ataccaggta cagctggata tcgcgatgca gtctggtaag ccgaaagaaa
    26041 tcgcagagaa aatggttgaa ggccgcatga agaaattcac cggcgaagtt tctctgaccg
    26101 gtcagccgtt cgttatggaa ccaagcaaaa ctgttggtca gctgctgaaa gagcataacg
    26161 ctgaagtgac tggcttcatc cgtttcgaag tgggtgaagg catcgagaaa gttgagactg
    26221 actttgcagc agaagttgct gcgatgtcca agcagtctta attatcaaaa aggagccgcc
    26281 tgagggcggc ttctttttgt gcccatcttg taaattcagc taacccttgt ggggctgcgc
    26341 tgaaaagcga cgtacaatgt cgctagtatt aattcatttc aatcgttgac agtctcagga
    26401 aagaaacatg gctaccaatg caaaacccgt ctataaacgc attctgctta agttgagtgg
    26461 cgaagctctg cagggcactg aaggcttcgg tattgatgca agcatactgg atcgtatggc
    26521 tcaggaaatc aaagaactgg ttgaactggg tattcaggtt ggtgtggtga ttggtggggg
    26581 taacctgttc cgtggcgctg gtctggcgaa agcgggtatg aaccgcgttg tgggcgacca
    26641 catggggatg ctggcgaccg taatgaacgg cttggcaatg cgtgatgcac tgcaccgcgc
    26701 ctatgtgaac gctcgtctga tgtccgctat tccattgaat ggcgtgtgcg acagctacag
    26761 ctgggcagaa gctatcagcc tgttgcgcaa caaccgtgtg gtgatcctct ccgccggtac
    26821 aggtaacccg ttctttacca ccgactcagc agcttgcctg cgtggtatcg aaattgaagc
    26881 cgatgtggtg ctgaaagcaa ccaaagttga cggcgtgttt accgctgatc cggcgaaaga
    26941 tccaaccgca accatgtacg agcaactgac ttacagcgaa gtgctggaaa aagagctgaa
    27001 agtcatggac ctggcggcct tcacgctggc tcgtgaccat aaattaccga ttcgtgtttt
    27061 caatatgaac aaaccgggtg cgctgcgccg tgtggtaatg ggtgaaaaag aagggacttt
    27121 aatcacggaa taattcccgt gatggataaa taagggtaag attccgcgta agtatcgcgg
    27181 gggcgtaagt ctggttataa ggcgttattg ttgcaggcag tttggtcacg gccagcgcgc
    27241 agcaaccgga gcgtacaaaa gtacgtgagg atggcgagca ctgctcgggg ccaaaatggc
    27301 aaataaaata gcctaataat ccagacgatt acccgtaata tgtttaatca gggctatact
    27361 tagcacactt ccactgtgtg tgactgtctg gtctgactga gacaagtttt caaggattcg
    27421 taacgtgatt agcgatatca gaaaagatgc tgaagtacgc atggacaaat gcgtagaagc
    27481 gttcaaaacc caaatcagca aaatacgcac gggtcgtgct tctcccagcc tgctggatgg
    27541 cattgtcgtg gaatattacg gcacgccgac gccgctgcgt cagctggcaa gcgtaacggt
    27601 agaagattcc cgtactctga aaatcaacgt gtttgatcgt tcaatgtctc cggccgttga
    27661 aaaagcgatt atggcctccg atctcggcct gaacccgaac tctgcgggta gcgacatccg
    27721 tgttccgctg ccgccgctga cggaagaacg tcgtaaagat ctgaccaaaa tcgttcgtgg
    27781 tgaagcagaa caagcgcgtg ttgcagtacg taatgtgcgt cgtgacgcga acgacaaagt
    27841 gaaagcactg ttgaaagata aagagatcag cgaagacgac gatcgccgtt ctcaggacga
    27901 tgtacagaaa ctgactgatg ctgcaatcaa gaaaattgaa gcggcgctgg cagacaaaga
    27961 agcagaactg atgcagttct gatttcttga acgacaaaaa cgccgctcag tagatccttg
    28021 cggatcggct ggcggcgttt tgctttttat tctgtctcaa ctctggatgt ttcatgaagc
    28081 aactcaccat tctgggctcg accggctcga ttggttgcag cacgctggac gtggtgcgcc
    28141 ataatcccga acacttccgc gtagttgcgc tggtggcagg caaaaatgtc actcgcatgg
    28201 tagaacagtg cttggaattc tctccccgct atgccgtaat ggacgatgaa gcgagtgcga
    28261 aacttcttaa aacgatgcta cagcaacagg ggagccgcac cgaagtctta agtgggcaac
    28321 aagccgcttg cgatatggca gcgcttgagg aggttgatca ggtgatggca gccattgttg
    28381 gtgctgctgg actgttacct acgcttgctg cgatccgcgc gggtaaaacc attttgctgg
    28441 ccaataaaga atcactggtt acctgcggac gtctgtttat ggacgccgta aagcagagca
    28501 aagcgcaatt gttaccggtc gatagcgaac ataacgctat ttttcagagt ttaccgcaac
    28561 ctatccagca taatctggga tacgctgacc ttgagcaaaa tggcgtggtg tccattttac
    28621 ttaccgggtc tggtggccct ttccgtgaga cgccattgcg cgatttggca acaatgacgc
    28681 ccgatcaagc ttgccgtcat ccgaactggt cgatgggacg taaaatttct gtcgattcgg
    28741 ctaccatgat gaacaaaggt ctggaataca ttgaagcgcg ttggctgttt aacgccagcg
    28801 ccagccagat ggaagtgctg attcacccgc agtcagtgat tcactcaatg gtgcgctatc
    28861 aggacggcag tgttctggcg cagctggggg aaccggatat gcgtacgcca attgcccaca
    28921 ccatggcatg gccgaatcgc gtgaactctg gcgtgaagcc gctcgatttt tgcaaactaa
    28981 gtgcgttgac atttgccgca ccggattatg agcgttatcc atgcctgaaa ctggcgatgg
    29041 aggcgttcga acaaggccag gcagcgacga cagcattgaa tgccgcaaac gaaatcaccg
    29101 ttgctgcttt tcttgcgcaa caaatccgct ttacggatat cgctgcgttg aatttatccg
    29161 tactggaaaa aatggatatg cgcgaaccac aatgtgtgga cgatgtgtta tctgttgatg
    29221 cgaacgcgcg tgaagtcgcc agaaaagagg tgatgcgtct cgcaagctga ggataatccg
    29281 gctacagaga gtcgcgctat ttgttagcgt agggcttcag tgatatagtc tgcgccatct
    29341 gatcgtaagt agttggcttt ataaggtcag gtatgccgtg gttttacacg gctttttttt
    29401 gtataggctt cagtattcct gagtaccgta aaccctgtca gggaataaaa aacgcgtgat
    29461 gttgtctgct actcaaccac ttagcgaaaa attgccagcg catggctgcc gtcatgttgc
    29521 gatcattatg gacggcaatg gtcgctgggc aaaaaagcaa gggaagattc gtgcctttgg
    29581 gcataaagcc ggggcaaaat ccgtccgccg ggctgtctct tttgcggcaa acaacggtat
    29641 tgaggcgtta acgctgtatg cctttagtag tgaaaactgg aaccgaccag cgcaggaagt
    29701 cagtgcgtta atggaactgt ttgtgtgggc gctcgatagc gaagtaaaaa gtctgcaccg
    29761 acataacgtg cgtctgcgta ttattggcga taccagtcgt tttaactcgc gtttgcaaga
    29821 acgtattcgt aaatctgaag cgctaacagc cggaaatacc ggtctgacgc tgaatattgc
    29881 ggcgaactac ggtggacgtt gggatatagt ccagggagtc aggcaactgg ctgaaaaggt
    29941 gcagcaagga aacctgcaac cagatcagat agatgaagag atgttaaacc agcatgtctg
    30001 tatgcatgaa ctggcccctg tagatttagt aattaggact gggggagagc atcgcattag
    30061 taactttttg ctttggcaaa ttgcctatgc cgaactttac tttacagatg ttctctggcc
    30121 cgatttcgat gaacaagact ttgaaggggc gttaaatgcc tttgctaatc gagagcgtcg
    30181 tttcggcggc accgagcccg gtgatgaaac agcctgatgg gggtcgcttt tgctgaagta
    30241 tcgcctgata tctgcttttg tgttaatacc cgtcgtcatc gcggcgttgt ttctgttgcc
    30301 gccggtgggg ttcgccattg taacgctggt ggtctgcatg ctggcagcgt gggaatgggg
    30361 acagcttagc ggttttacca ctcgttcgca gcgagtatgg ttggcggtgt tatgcgggtt
    30421 attgttggcg ctgatgcttt ttctgttgcc ggaatatcac cgaaatattc atcaaccgct
    30481 ggttgaaatc tcactttggg cttcgttggg ttggtggatt gtcgcgctat tgctggtgct
    30541 gttttaccca ggttccgcag caatctggcg taactctaaa acattgcgcc ttatttttgg
    30601 cgtgctaacc attgttccct tcttctgggg catgctggcg ttacgggcct ggcactatga
    30661 cgagaatcat tacagtggcg caatatggct gctctatgtc atgatcctgg tatggggcgc
    30721 tgactccggc gcatatatgt ttggcaaatt gtttggtaaa cataagctgg caccgaaggt
    30781 ttctccgggt aaaacctggc aaggctttat aggtggactc gctactgcag cggtaatctc
    30841 atggggttat ggcatgtggg cgaatctcga cgtcgctccc gtcaccttac tcatttgctc
    30901 tattgtcgca gcgttagcct cagtgctcgg cgatctgacc gagagtatgt ttaagcgtga
    30961 agcaggaatt aaggacagcg gtcatttaat tccaggacac ggtggtattt tagatcgtat
    31021 tgatagcctg acggctgcgg taccggtctt tgcttgcttg ttgttactgg tattcaggac
    31081 gctttaacgg aaggtaatat gctgagtttt ctctgggatt tggcttcgtt catcgttgca
    31141 ctgggtgtac ttatcaccgt gcatgaattt ggtcatttct gggttgcccg gcgttgtggt
    31201 gttcgcgttg agcgtttctc aatagggttt ggtaaggcgc tctggcggcg aactgataag
    31261 ctcggcaccg aatatgttat cgccctgatc ccgttgggcg gttatgtcaa aatgctggat
    31321 gagcgcgcag aaccggtcgt tccggaactc cgccaccatg ccttcaataa taaatctgtc
    31381 ggccaacgag cggcgattat tgccgcaggt ccggttgcaa acttcatttt tgctatcttt
    31441 gcctactggc tggtttttat tattggtgtg cctggcgtac gtccggtggt tggcgaaata
    31501 gcagccaatt cgatagctgc ggaagcacaa attgcaccag gtacggaact aaaagccgta
    31561 gatggtatcg aaacgcctga ttgggatgcc gtgcgtttgc agttggtcga taaaattggc
    31621 gatgaaagca ccaccattac agtagcgcca tttggcagcg accaacggcg ggatgtaaag
    31681 ctcgatttac gtcactgggc gtttgagcct gataaagaag atccggtatc ttcgctgggg
    31741 attcgtcctc gtgggccgca aattgaacct gtactggaaa atgtgcagcc aaactcggcg
    31801 gcaagcaagg caggtttgca agcaggcgac aggatcgtta aagtcgatgg tcagccctta
    31861 acgcagtggg tgacctttgt gatgtttgtc cgggataacc cgggtaaatc cttagcgtta
    31921 gaaatcgaaa ggcaggggag tcccttgtct ttgacattaa tcccggagag taaaccgggt
    31981 aatggtaaag cgattggttt tgtcggtatt gagccgaaag tcattccttt gccagatgag
    32041 tataaagttg tacgccagta tgggccgttc aacgccatcg tcgaagccac ggacaaaacg
    32101 tggcagctga tgaagctgac ggtcagtatg ctgggaaaat tgatcaccgg tgatgtgaaa
    32161 ctgaacaacc tcagtgggcc gatctctatc gccaaggggg ctgggatgac agcggaactc
    32221 ggggtagttt attacctgcc gtttcttgcg cttattagcg tgaacttagg gataattaac
    32281 ctgtttccgt tgcccgtact tgacgggggg catctgctgt tccttgcgat cgaaaagatc
    32341 aagggcggac cggtatccga gcgggttcaa gacttttgtt atcgcattgg ctcgattctg
    32401 ctggtgctgt taatggggct tgcacttttc aatgatttct ctcggttatg agagttagtt
    32461 aggaagaacg cataataacg atggcgatga aaaagttgct catagcgtcg ctgctgttta
    32521 gcagcgccac cgtatacggt gctgaagggt tcgtagtgaa agatattcat ttcgaaggcc
    32581 ttcagcgtgt cgccgttggt gcggccctcc tcagtatgcc ggtgcgtaca ggcgacacgg
    32641 ttaatgatga agatatcagt aataccattc gcgctctgtt tgctaccggc aactttgagg
    32701 atgttcgcgt ccttcgtgat ggtgataccc ttctggttca ggtaaaagaa cgtccgacca
    32761 ttgccagcat tactttctcc ggtaacaaat cggtgaaaga tgacatgctg aagcaaaacc
    32821 tcgaggcttc tggtgtgcgt gtgggcgaat ccctcgatcg caccaccatt gccgatatcg
    32881 agaaaggtct ggaagacttc tactacagcg tcggtaaata tagcgccagc gttaaagctg
    32941 tcgtgacccc gctgccgcgc aaccgtgttg acctaaaact ggtgttccag gaaggtgtgt
    33001 cagctgaaat ccagcaaatt aacattgttg gtaaccatgc tttcaccacc gacgaactga
    33061 tctctcattt ccaactgcgt gacgaagtgc cgtggtggaa tgtggtaggc gatcgtaaat
    33121 accagaaaca gaaactggcg ggcgaccttg aaaccctgcg cagctactat ctggaccgcg
    33181 gttatgcccg tttcaacatc gactctaccc aggtcagtct gacgccagat aaaaaaggta
    33241 tttacgtcac ggtgaacatc accgaaggcg atcagtacaa gctttctggc gttgaagtga
    33301 gcggcaacct tgccgggcac tccgctgaaa ttgagcagct gactaagatc gagccgggcg
    33361 agctgtataa cggcaccaaa gtgaccaaaa tggaagatga cattaaaaag cttctcggtc
    33421 gctatggtta tgcctatccg cgcgtacagt cgatgcctga aattaacgat gccgacaaaa
    33481 ccgttaaatt acgcgtgaac gttgatgcgg gtaaccgttt ctacgtgcgt aagatccgtt
    33541 ttgaaggtaa cgatacctcg aaagatgccg tcctgcgtcg cgaaatgcgt cagatggaag
    33601 gtgcatggtt ggggagcgat ctggtcgatc agggtaagga gcgtctgaat cgtctgggct
    33661 tctttgaaac tgtcgatacc gatacccaac gtgttccggg tagcccggac caggttgatg
    33721 tagtctacaa ggtgaaagaa cgtaacactg gtagcttcaa cttcgggatt ggttacggta
    33781 ctgaaagtgg cgtgagcttc caggctggcg tgcaacagga taactggtta ggtacaggtt
    33841 atgctgttgg tatcaacggg accaaaaacg attaccagac ctatgctgaa ctgtcggtaa
    33901 ccaacccgta cttcaccgta gatggcgtaa gcctcggtgg tcgtctcttc tataatgact
    33961 tccaggcaga tgacgccgac ctgtccgact ataccaacaa gagttatggt acagacgtga
    34021 cgttgggctt cccgattaac gaatataact cgctgcgtgc aggtctgggt tatgtacata
    34081 actccctgtc caacatgcag cctcaggttg cgatgtggcg ttatctgtac tctatgggtg
    34141 aacatccgag cacctctgat caggataaca gcttcaaaac ggacgacttc acgttcaact
    34201 atggttggac ctataacaag cttgaccgtg gttacttccc gacagatggt tcacgtgtca
    34261 acctgaccgg taaagtgacc attcctggat cggataacga atactacaaa gtgacgttag
    34321 acacggcgac ttatgtgccg atcgatgacg atcacaaatg ggttgttctg ggtcgtaccc
    34381 gctggggtta tggtgatggt ttaggcggca aagagatgcc gttctacgag aacttctatg
    34441 ccggtggttc cagcaccgtg cgtggcttcc agtccaatac cattggtccg aaagcagttt
    34501 acttcccgca tcaggccagt aattatgatc cggactatga ttacgaatgt gcgactcagg
    34561 acggcgcgaa agacctgtgt aaatcggatg atgctgtagg cggtaacgcc atggcggttg
    34621 ccagcctcga gttcatcacc ccgacgccgt ttattagcga taagtatgct aactcggttc
    34681 gtacttcatt cttctgggat atgggtaccg tttgggatac aaactgggat tccagccaat
    34741 attctggata tccggactat agtgatccaa gcaatatccg tatgtctgcg ggtatcgcat
    34801 tacaatggat gtccccattg gggccgttgg tgttctccta cgcccagccg ttcaaaaagt
    34861 acgatggaga caaggcagaa cagttccagt ttaacatcgg taaaacctgg taagtgttct
    34921 ccacaaagga atgtagtggt agtgtagcga tgactttagg cgatcaatat aagatcgccg
    34981 ggccacgcaa agaactgcac cctccggtgc aaatgggatg gtaaggagtt tattgtgaaa
    35041 aagtggttat tagctgcagg tcttggttta gcactggcaa cttctgctca ggcggctgac
    35101 aaaattgcaa tcgtcaatat gggcagcctg ttccagcagg tagcgcagaa aaccggtgtt
    35161 tctaacacgc tggaaaatga gttcaaaggc cgtgccagcg aactgcagcg tatggaaacc
    35221 gatctgcagg ctaaaatgaa aaaactgcag tccatgaaag cgggcagcga tcgcaccaaa
    35281 ctggaaaaag acgtgatggc tcagcgccag acttttgctc agaaagcgca ggcttttgag
    35341 caggatcgcg cacgtcgttc caacgaagaa cgcggcaaac tggttactcg tatccagact
    35401 gctgtgaaat ccgttgccaa cagccaggat atcgatctgg ttgttgatgc aaacgccgtt
    35461 gcttacaaca gcagcgatgt aaaagacatc actgccgacg tactgaaaca ggttaaataa
    35521 gtaatgcctt caattcgact ggctgattta gcgcagcagt tggatgcaga actacacggt
    35581 gatggcgata tcgtcatcac cggcgttgcg tccatgcaat ctgcacaaac aggtcacatt
    35641 acgttcatgg ttaacccaaa ataccgtgag catttaggct tgtgccaggc gtccgcggtt
    35701 gtcatgaccc aggacgatct tcctttcgcg aaaagtgccg cgctggtagt gaagaatccc
    35761 tacctgactt acgcgcgcat ggcgcaaatt ttagatacca cgccgcagcc cgcgcagaac
    35821 attgcaccca gtgcggtgat cgacgcgacg gcgaagctgg gtaacaacgt atcgattggc
    35881 gctaacgcgg tgattgagtc cggcgttgaa ctgggcgata acgtgattat cggtgccggt
    35941 tgcttcgtag gtaaaaacag caaaatcggt gcaggttcgc gtctctgggc gaacgtaacc
    36001 atttaccatg agatccagat cggtcagaat tgcctgatcc agtccggaac agtggtaggc
    36061 gcagacggct ttggttatgc caacgatcgt ggtaactggg tgaagatccc acagattggt
    36121 cgcgtaatta ttggcgatcg cgtggagatc ggtgcctgca caaccatcga tcgcggcgcg
    36181 ctggatgaca ctgttattgg caatggcgtg atcattgata accagtgcca gattgcacat
    36241 aacgtcgtga ttggcgacaa tacggcggtt gccggtggcg tcattatggc gggcagcctg
    36301 aaaattggtc gttactgcat gatcggcgga gccagcgtaa tcaacgggca tatggaaata
    36361 tgcgacaaag tgacggttac gggcatgggt atggtgatgc gtcccatcac tgaaccaggc
    36421 gtctattcct caggcattcc gctgcaaccc aacaaagtct ggcgcaaaac cgctgcactg
    36481 gtgatgaaca ttgatgacat gagcaagcgt ctgaaatcgc ttgagcgcaa ggttaatcaa
    36541 caagactaac gttccatctt ttgttcgcca aactttacgg cctgtctcat tcttacgatt
    36601 gcggcaggcc gtgttattat tgtcgtttct tatattttga caggaagagt atcttgacta
    36661 ctaacactca tactctgcag attgaagaga ttttagaact tctgccgcac cgtttcccgt
    36721 tcttactggt ggatcgcgtg ctggattttg aagaaggtcg ttttctgcgc gcagtaaaaa
    36781 atgtctctgt caatgagcca ttcttccagg gccatttccc tggaaaaccg attttcccgg
    36841 gtgtgctgat tctggaagca atggcacagg caacaggtat tctggcgttt aaaagcgtag
    36901 gaaaactgga accgggtgag ctgtactact tcgctggtat tgacgaagcg cgcttcaagc
    36961 gcccggtcgt gcctggcgat caaatgatca tggaagtcac tttcgaaaaa acgcgccgcg
    37021 gcctgacccg ttttaaaggg gttgctctgg tcgatggtaa agtagtttgc gaagcaacga
    37081 tgatgtgtgc tcgtagccgg gaggcctgat acgtgattga taaatccgcc tttgtgcatc
    37141 caaccgccat tgtggaagag ggcgcgtcaa ttggcgcgaa cgcacacatt ggtccttttt
    37201 gtatcgttgg accccatgtc gaaattggtg agggtaccgt actgaaatct cacgttgtcg
    37261 tgaatggtca tactaaaatt ggccgcgata atgagattta tcagtttgcc tccatcggcg
    37321 aagttaacca ggatttgaaa tatgctggcg aaccgacccg tgtggaaatc ggcgatcgta
    37381 accgcattcg cgaaagcgtc accattcatc gtggcacagt ccagggcggt ggattgacga
    37441 aggtgggcag cgacaactta ctgatgatca acgcgcacat tgcgcacgat tgtacggtag
    37501 gtaaccgctg tattctcgcc aacaacgcaa cgctggcggg tcacgtatcg gttgacgact
    37561 tcgcgatcat cggcggcatg accgcagtcc atcagttctg catcattggt gcgcatgtga
    37621 tggttggcgg ctgctccggt gtggcgcagg acgtccctcc ttatgtcatt gcgcagggta
    37681 accacgcaac gccgttcggt gtcaatatcg aagggctgaa gcgccgcgga ttcagccgtg
    37741 aggcgattac cgctatccgc aatgcgtata agctaattta tcgtagcggt aaaacgctcg
    37801 atgaagtgaa accggaaatt gctgaactgg cggaaacata tccggaagtg aaagccttta
    37861 ccgatttctt tgcacgctca acgcgcggtc tgattcgtta atgactgacc agcgtccatt
    37921 aacgattgcc ctggtcgccg gagaaacctc cggcgatatc ctgggggccg gtttaatccg
    37981 cgctctgaaa gaacgtgtgc ccaacgcccg ctttgttggt gttgccgggc cacgaatgca
    38041 ggctgaaggc tgcgaagcct ggtacgaaat ggaagaactg gcggtgatgg gcattgttga
    38101 agtgctcggt cgtctgcgtc gcttactgca tattcgtgcc gatctgacaa agcgttttgg
    38161 cgaactgaag ccagatgttt ttgttggtat tgatgcgccc gacttcaata ttactcttga
    38221 aggtaacctc aaaaagcagg gtatcaaaac cattcattac gtcagtccat ccgtctgggc
    38281 gtggcgacag aaacgcgttt tcaaaatagg cagagccacc gatctggtgc tcgcatttct
    38341 gcctttcgaa aaagcgtttt atgacaaata caatgtaccg tgccgcttta tcggtcatac
    38401 catggctgat gccatgccgt tagatccaga taaaaatggt gcccgtgatg tgctggggat
    38461 cccttacgat gcccactgtc tggcattgtt gccgggcagc cgtggtgcag aagtcgaaat
    38521 gcttagtgcc gatttcttga aaactgccca gcttttgcgc cagacatatc cggatctcga
    38581 aatcgtggtg ccgctggtga atgccaaacg ccgcgagcag tttgaacgca tcaaagctga
    38641 agtcgcgcca gacctgtcgg ttcatttgct ggatggaatg ggccgtgagg cgatggtcgc
    38701 cagcgatgcg gcactactgg cgtcggggac ggcagccctg gagtgtatgc tggcgaaatg
    38761 cccgatggtg gtgggatatc gcatgaagcc ttttaccttc tggttggcga agcggctggt
    38821 gaaaactgat tatgtctcgc tgccaaatct gctggcgggc agagagttag tcaaagagtt
    38881 attgcaggaa gagtgtgagc cgcaaaaact ggctgcggcg ctgttaccgc tgttggcgaa
    38941 cgggaaaacc agccacgcga tgcacgatac cttccgtgaa ctgcatcagc agatccgctg
    39001 caatgccgat gagcaggcgg cacaagccgt tctggagtta gcacaatgat cgaatttgtt
    39061 tatccgcaca cgcagctggt tgcgggtgtg gatgaagtcg gacgcgggcc gttagttggc
    39121 gcggtcgtca ccgctgcggt gatccttgac ccggcgcgcc cgattgccgg gctgaatgat
    39181 tccaaaaagc tgagcgaaaa acgccgtctg gcgctctatg aagagatcaa agagaaagcg
    39241 ttgagctgga gtctgggccg cgcggaaccc cacgaaatcg acgagctgaa cattcttcat
    39301 gcgaccatgc tggcgatgca gcgtgccgtc gctgggctgc atattgcgcc ggaatatgtg
    39361 ttgattgatg gtaaccgctg cccgaaatta ccgatgcctg cgatggctgt ggtgaaaggg
    39421 gatagccgcg taccagaaat cagtgccgcg tctatcctgg cgaaagtgac gcgtgacgcc
    39481 gaaatggcgg cgctggatat tgttttcccg caatatggtt ttgcccaaca taaagggtat
    39541 ccaaccgctt ttcatctgga aaaactggct gaatatggcg cgactgaaca tcatcgtcgc
    39601 agctttgggc ctgtcaaacg cgcactggga cttgcgtcct gattcttgtg tcgagattaa
    39661 gtaaaccgga atctgaagat gtctgaacca cgtttcgtac acctgcgggt gcacagcgac
    39721 tactcgatga tcgatggctt ggccaaaacc gcaccgctgg taaaaaaggc ggcggcgttg
    39781 ggtatgccag cactggcgat caccgatttc accaaccttt gcggtctggt gaagttctac
    39841 ggagcgggac atggcgcagg gattaagcct atcgtcgggg cagattttaa tgtccagtgc
    39901 gacctgctgg gtgatgagtt aacccacctg acggtactgg cggcgaacaa taccggctat
    39961 cagaatctgg cgttgctgat ctcaaaagcg tatcagcgcg ggtacggtgc cgccgggccg
    40021 atcatcgatc gcgactggct tatcgaatta aacgaagggt tgatccttct ttccggcggg
    40081 cgcatgggcg acgtcggacg cagtcttttg cgtggtaaca gcgcgctggt agatgagtgt
    40141 gtcgcgtttt atgaagaaca cttcccggat cgctattttc tcgagctgat ccgcaccggc
    40201 aggccggatg aagaaagcta tctgcacgcg gcggtggaac tggcggaagc gcgcggtttg
    40261 cccgtcgtgg cgactaacga tgtgcgcttt atcgacagca gcgactttga cgcacacgaa
    40321 atccgcgtcg cgatccacga cggctttacc ctcgacgatc ctaaacgccc gcgtaactat
    40381 tcgccgcagc aatatatgcg tagcgaagag gagatgtgcg agctgtttgc cgacatccca
    40441 gaagcccttg ccaacaccgt tgagatcgcc aaacgctgta acgtaactgt gcgtcttggt
    40501 gaatacttcc tgccgcagtt cccgaccggg gacatgagca ccgaagatta tctggtcaag
    40561 cgtgcaaaag aaggcctgga agagcgtctg acatttttat ttcctgacga ggaagaacgt
    40621 cttaaacgcc gcccggaata tgacgaacgt ctggagactg aacttcaggt tatcaaccag
    40681 atgggcttcc cgggctactt cctcatcgtt atggaattta tccagtggtc gaaagataac
    40741 ggcgtaccgg tagggccagg ccgtggctcc ggtgcgggtt cactggtagc ctacgcgctg
    40801 aaaatcaccg acctcgatcc gctggaattt gacctgctgt tcgaacgttt ccttaacccg
    40861 gaacgtgtct ccatgcctga cttcgacgtt gacttctgta tggagaaacg cgatcaggtt
    40921 atcgaacacg tggcggacat gtacggtcgt gatgcggtat cgcagattat caccttcggt
    40981 acgatggcgg cgaaagcggt gatccgcgac gtaggccgcg tgctggggca tccgtacggc
    41041 tttgtcgacc gtatctcgaa actgatcccg cccgatccgg ggatgacgct ggcgaaagcg
    41101 tttgaagccg agccgcagct gccggaaatc tacgaagcgg atgaagaagt taaggcgctg
    41161 atcgacatgg cgcgcaaact ggaaggggtc acccgtaacg ccggtaagca cgccggtggg
    41221 gtggttatcg cgccgaccaa aattaccgat tttgcgccgc tttattgcga tgaagagggc
    41281 aaacatccgg tcacccagtt tgataaaagc gacgttgaat acgccggact ggtgaagttc
    41341 gacttccttg gtttgcgtac gctcaccatc attaactggg cgctggagat gatcaacaag
    41401 cggcgggcga agaatggcga gccaccgctg gatatcgccg cgatcccgct ggatgacaag
    41461 aaaagcttcg acatgctgca acgctcggaa accacggcgg tattccagct tgaatcgcgc
    41521 ggcatgaagg acctgatcaa gcgtctgcaa cctgactgct tcgaagatat gatcgcactg
    41581 gtggcactgt tccgccctgg tccgttgcag tcagggatgg tggataactt tatcgaccgt
    41641 aaacatggtc gtgaagagat ctcctatccg gacgtgcagt ggcagcatga aagcctgaaa
    41701 tcggtactgg agccaaccta cggcattatc ttgtatcagg aacaggtcat gcagattgcc
    41761 caggtgcttt ctggttatac cctcggtggc gcggatatgc tgcgccgtgc gatgggtaag
    41821 aaaaagccgg aagagatggc taagcagcgt tctgtatttg ctgaaggtgc agaaaagaac
    41881 ggaatcaacg ccgaactggc gatgaaaatc ttcgacctgg tggagaaatt cgcgggttac
    41941 ggatttaaca aatcgcactc tgcggcctat gctttggtgt catatcaaac gttatggctg
    42001 aaagcgc
//
LOCUS       DADLSB010000016        41546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.016, whole genome shotgun sequence.
ACCESSION   DADLSB010000016 DADLSB010000000
VERSION     DADLSB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.016"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            13..89
                     /locus_tag="JN331_000879"
     tRNA            13..89
                     /locus_tag="JN331_000879"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47..49,aa:Met,seq:cat)
     gene            138..212
                     /locus_tag="JN331_000880"
     tRNA            138..212
                     /locus_tag="JN331_000880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:170..172,aa:Gln,seq:ctg)
     gene            250..324
                     /locus_tag="JN331_000881"
     tRNA            250..324
                     /locus_tag="JN331_000881"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:282..284,aa:Gln,seq:ctg)
     gene            complement(478..1653)
                     /gene="ubiF"
                     /locus_tag="JN331_000882"
     CDS             complement(478..1653)
                     /gene="ubiF"
                     /locus_tag="JN331_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY7259092.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVIPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1799..3223
                     /gene="miaB"
                     /locus_tag="JN331_000883"
     CDS             1799..3223
                     /gene="miaB"
                     /locus_tag="JN331_000883"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY7259093.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3337..4416
                     /locus_tag="JN331_000884"
     CDS             3337..4416
                     /locus_tag="JN331_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY7259094.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYQAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            4413..4880
                     /gene="ybeY"
                     /locus_tag="JN331_000885"
     CDS             4413..4880
                     /gene="ybeY"
                     /locus_tag="JN331_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY7259095.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4970..5848
                     /gene="corC"
                     /locus_tag="JN331_000886"
     CDS             4970..5848
                     /gene="corC"
                     /locus_tag="JN331_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY7259096.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5873..7411
                     /gene="lnt"
                     /locus_tag="JN331_000887"
     CDS             5873..7411
                     /gene="lnt"
                     /locus_tag="JN331_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY7259097.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7808..8716
                     /gene="gltI"
                     /locus_tag="JN331_000888"
     CDS             7808..8716
                     /gene="gltI"
                     /locus_tag="JN331_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAY7259098.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8886..9626
                     /gene="gltJ"
                     /locus_tag="JN331_000889"
     CDS             8886..9626
                     /gene="gltJ"
                     /locus_tag="JN331_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY7259099.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9626..10300
                     /gene="gltK"
                     /locus_tag="JN331_000890"
     CDS             9626..10300
                     /gene="gltK"
                     /locus_tag="JN331_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY7259100.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10300..11025
                     /gene="gltL"
                     /locus_tag="JN331_000891"
     CDS             10300..11025
                     /gene="gltL"
                     /locus_tag="JN331_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY7259101.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            11143..12078
                     /gene="rihA"
                     /locus_tag="JN331_000892"
     CDS             11143..12078
                     /gene="rihA"
                     /locus_tag="JN331_000892"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY7259102.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            12162..13832
                     /locus_tag="JN331_000893"
     CDS             12162..13832
                     /locus_tag="JN331_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY7259103.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(13892..15343)
                     /gene="djlC"
                     /locus_tag="JN331_000894"
     CDS             complement(13892..15343)
                     /gene="djlC"
                     /locus_tag="JN331_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY7259104.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15340..16047)
                     /locus_tag="JN331_000895"
                     /pseudo
     CDS             complement(15340..16047)
                     /locus_tag="JN331_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            16149..16703
                     /locus_tag="JN331_000896"
     CDS             16149..16703
                     /locus_tag="JN331_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7259105.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16713..18140)
                     /locus_tag="JN331_000897"
     CDS             complement(16713..18140)
                     /locus_tag="JN331_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY7259106.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(18137..18844)
                     /locus_tag="JN331_000898"
     CDS             complement(18137..18844)
                     /locus_tag="JN331_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7259107.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHLSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            19008..19988
                     /locus_tag="JN331_000899"
     CDS             19008..19988
                     /locus_tag="JN331_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY7259108.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWY
                     KQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAG
                     EGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECND
                     ATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(20058..20540)
                     /locus_tag="JN331_000900"
     CDS             complement(20058..20540)
                     /locus_tag="JN331_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY7259109.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20775..23357
                     /gene="leuS"
                     /locus_tag="JN331_000901"
     CDS             20775..23357
                     /gene="leuS"
                     /locus_tag="JN331_000901"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY7259110.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23372..23953
                     /gene="lptE"
                     /locus_tag="JN331_000902"
     CDS             23372..23953
                     /gene="lptE"
                     /locus_tag="JN331_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY7259111.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPINAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            23953..24984
                     /gene="holA"
                     /locus_tag="JN331_000903"
     CDS             23953..24984
                     /gene="holA"
                     /locus_tag="JN331_000903"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY7259112.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24986..25627
                     /gene="nadD"
                     /locus_tag="JN331_000904"
     CDS             24986..25627
                     /gene="nadD"
                     /locus_tag="JN331_000904"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY7259113.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25651..26262
                     /locus_tag="JN331_000905"
                     /pseudo
     CDS             25651..26262
                     /locus_tag="JN331_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            26522..26839
                     /gene="rsfS"
                     /locus_tag="JN331_000906"
                     /gene_synonym="rsfA"
     CDS             26522..26839
                     /gene="rsfS"
                     /locus_tag="JN331_000906"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY7259114.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26843..27310
                     /gene="rlmH"
                     /locus_tag="JN331_000907"
     CDS             26843..27310
                     /gene="rlmH"
                     /locus_tag="JN331_000907"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY7259115.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27341..29242
                     /gene="mrdA"
                     /locus_tag="JN331_000908"
     CDS             27341..29242
                     /gene="mrdA"
                     /locus_tag="JN331_000908"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY7259116.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKIDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKQWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29245..30357
                     /gene="mrdB"
                     /locus_tag="JN331_000909"
                     /gene_synonym="rodA"
     CDS             29245..30357
                     /gene="mrdB"
                     /locus_tag="JN331_000909"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY7259117.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30368..31456
                     /gene="rlpA"
                     /locus_tag="JN331_000910"
     CDS             30368..31456
                     /gene="rlpA"
                     /locus_tag="JN331_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY7259118.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31595..32806
                     /gene="dacA"
                     /locus_tag="JN331_000911"
     CDS             31595..32806
                     /gene="dacA"
                     /locus_tag="JN331_000911"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY7259119.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALDLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32917..33180
                     /locus_tag="JN331_000912"
     CDS             32917..33180
                     /locus_tag="JN331_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY7259120.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33281..33922
                     /gene="lipB"
                     /locus_tag="JN331_000913"
     CDS             33281..33922
                     /gene="lipB"
                     /locus_tag="JN331_000913"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY7259121.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            34181..35134
                     /locus_tag="JN331_000914"
     CDS             34181..35134
                     /locus_tag="JN331_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY7259122.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPNPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            35343..36308
                     /gene="lipA"
                     /locus_tag="JN331_000915"
     CDS             35343..36308
                     /gene="lipA"
                     /locus_tag="JN331_000915"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY7259123.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36409..36612)
                     /gene="tatE"
                     /locus_tag="JN331_000916"
     CDS             complement(36409..36612)
                     /gene="tatE"
                     /locus_tag="JN331_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY7259124.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36741..37529)
                     /locus_tag="JN331_000917"
     CDS             complement(36741..37529)
                     /locus_tag="JN331_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY7259125.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37622..38005
                     /gene="crcB"
                     /locus_tag="JN331_000918"
     CDS             37622..38005
                     /gene="crcB"
                     /locus_tag="JN331_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY7259126.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(38059..38268)
                     /gene="cspE"
                     /locus_tag="JN331_000919"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(38059..38268)
                     /gene="cspE"
                     /locus_tag="JN331_000919"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7259127.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38443..39003)
                     /locus_tag="JN331_000920"
     CDS             complement(38443..39003)
                     /locus_tag="JN331_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY7259128.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39590..40975
                     /gene="dcuC"
                     /locus_tag="JN331_000921"
     CDS             39590..40975
                     /gene="dcuC"
                     /locus_tag="JN331_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7259129.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHNVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(41016..41357)
                     /locus_tag="JN331_000922"
                     /pseudo
     CDS             complement(41016..41357)
                     /locus_tag="JN331_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT        10425 a        10478 c        10356 g        10287 t
ORIGIN      
        1 aagaaataat ctggctacgt agctcagttg gttagagcac atcactcata atgatggggg
       61 cacaggttcg aatcccgtcg tagccaccaa attctgaatg tgtcgaatac gttcggcaaa
      121 ttcaaaaacc aatttgttgg ggtatcgcca agcggtaagg caccggattc tgattccggc
      181 attccgaggt tcaaatcctc gtaccccagc caatttattc aagacgctta catcgtaagt
      241 gcacccagtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag
      301 gttcgaatcc tcgtacccca gccacattaa aaaagctcgc ttcggcgagc tttttgcttt
      361 tctgcgttca ttcaatgtcg aatgcgatgt tgacacgtct tatccttcaa tgtcggatgc
      421 gacgctgccg cgtcttatcc gacctacggt tggcacgcat ccggcaatgt tgtaaggcta
      481 caaccctaac gcatatttca gcgcctgacg tttcaacacg ccagcacgct ctgccgccat
      541 taacccgaga ttacgcacaa aacgcagcgg cggcagatta ttactgaatc cggcataaaa
      601 cagatccata ccactttgca taatgaagtt atccgccatg cgccgcatct ggtaacgctt
      661 gaggacagga taactggccc acgcttcacc gtagctgcgg gcattcacca gaacatcaat
      721 cagggcatcg acatcacgat aaccaagatt caccccctgc cccgccagcg gatggatggt
      781 atgcgcggca tcgcccacca gcgcaagccc cggctgcaca tactgcaacg catgtcggcg
      841 cgtcagcgga aacgcaccag cggcaagcgg tataacgtaa cccagacgcg acgggaaatg
      901 cttcgcgatt tccgcctgga gttgcgccat attcatattc tgcaactggc gaatacgcgc
      961 cggagagtca taccacacca gcgatgccca gttatcaaac aacggcagaa acgcacgcgg
     1021 tccgtccggg gtaaattgct gccaggtgct gtcgccggga tcgttctcac actgtacgct
     1081 aatcaacatg cacgactgcg catactgcca ggcatgaacg ccaattcccg ccatctgccg
     1141 cacctgagaa tttgcgccgt cggcaccaat caccagtttc gcgcggatcg tttcaccgcc
     1201 cttcagttcc agctcctgaa gatcattatc tcgatgtaat gcaatcagcg aggttggcac
     1261 acgtaacgtt actttcggat gcgcttccag cgcctgccac aacgcctgtt gcaggactgt
     1321 gttttccacc atgtagccaa gcaacggcaa ctttaattcg gctgcgtcaa ataccacatg
     1381 tgccgtttcc cactcccagg tttccagtct gcggtaagga tggcaacgca tagcctgtac
     1441 tgcatcccag acccctaacc ctttaagcaa tgataccgaa gccgcgctga tcgccgagat
     1501 ccgcacgtcc ggttggctgt cagcgacaaa cggtgcaggt tctgcgtgct cgatcaccgt
     1561 taccgaaaat ccgtgctgtg ccagccccag cgccagtgcg ccgccgacca ttcctccgcc
     1621 gacaatggca atttccgttg gttgatttgt catggtcaat catcctgtta gcaatatgct
     1681 ttaagtttac aggattttta cccccaaggg ctgataacgc tcatactggt cacaacggca
     1741 gcaaagcatt acactatgcg ccctgcattc ctggctacta tttcgcaaga gcaagtcgat
     1801 gaccaaaaaa ctccatatta aaacctgggg ctgtcagatg aacgagtacg attcatcgaa
     1861 gatggccgat ctgctggatg ccacccacgg ctatcaactg accgacgtgg cggaagaagc
     1921 ggatgtgcta ctgctgaaca cctgctcaat ccgcgagaag gctcaggaaa aagtcttcca
     1981 tcagttgggt cgctggaaac tgttaaaaga gaagaatcca gacctgatta tcggcgtcgg
     2041 tggctgcgtg gcatcgcaag aaggcgagca cattcgccag cgcgcccact atgtcgatat
     2101 tatttttggg ccgcaaacgc tgcaccgtct gccggagatg atcaactccg tgcgcggcga
     2161 ccgcagcccg gttgtagata tcagcttccc ggaaatcgag aagtttgacc gtctgccgga
     2221 accgcgcgcc gaagggccga ccgcgtttgt ctccatcatg gaaggctgca ataaatattg
     2281 cacctactgc gtggtgcctt acacccgtgg tgaagaggta agccgtccgt ctgacgatat
     2341 tctgtttgag attgcccagc ttgcggctca gggcgtgcgt gaagtcaacc tgctcggtca
     2401 gaatgtgaat gcctggcgtg gtgagaacta cgacggcacc accggatcgt ttgccgatct
     2461 gttgcgtctg gttgctgcga tcgacgggat cgatcgtatt cgctttacca ccagccatcc
     2521 gatcgaattc accgacgata tcatcgaagt gtaccgcgac acgcctgagc tggtgagctt
     2581 cctgcatctg ccggtacaga gcggttccga tcgcattctg aacctgatgg gacgtaccca
     2641 tacggcgctg gagtacaaag cgatcatccg taaactgcgt gcggcgcgtc cggatattca
     2701 gatcagttct gacttcattg ttggcttccc tggcgaaacc accgaagact tcgagaaaac
     2761 gatgaagctg attgccgacg tcaatttcga catgagctac agctttatct tctctgcacg
     2821 tccaggaacg cctgccgccg atatggttga tgatgttccg gaagaagaga agaagcagcg
     2881 tctgtatatt ctgcaagaac gtatcaatca gcaagcgatg gcatggagcc gtcggatgct
     2941 cggcaccacc cagcgtattc tggtagaagg gacttcgcgt aagagcatca tggagctttc
     3001 cgggcgtacc gaaaataacc gcgtggtcaa cttcgagggc acaccggata tgatcggtaa
     3061 attcgtcgat gtagaaatta ccgacgtcta cccgaactct ctgcgcggta aagtggtgcg
     3121 aactgaagat gaaatgggtc tgcgcatggc agaaacaccg gagtcggtga ttgcccgcac
     3181 ccgcaaagaa aacgaccttg gcgtgggtta ttatcagccg taattctcag gcctgccgtt
     3241 ccggtgggcc ttgttttttc tctctttatc cccatcttta tcgcaatgct tgcgcctttt
     3301 tcctgtggcg tgaataattt ccttataggc cggtgaatgt tcagcttccg gcggcataca
     3361 gataagagga acggtttgaa catagacact cgcgaaatca ccctggagcc agcagacaat
     3421 gcgcgtctgt tgagcctgtg cggcccgttt gatgacaaca tcaagcagct cgaacgccgt
     3481 ctcggcatcg agatcaatcg ccgcgataac cactttaaac tgaccggccg tccgatttgc
     3541 gtcaccgctg cggcagacat tctgcgtagc ctgtatgtcg atactgcccc gatgcgcggt
     3601 cagattcagg atatcgaacc ggaacagatc caccttgcga ttaaagaagc gcgggtactg
     3661 gagcaaagcg cggagagcgt gccggagtac ggcaaagcgg tcaatatcaa aactaaacgc
     3721 ggcgtaatta agccgcgcac gccaaaccag gcgcagtaca ttgccaatat tctcgaccat
     3781 gacattacct tcggcgttgg cccggcgggt acgggtaaaa cctaccaggc agtggctgcg
     3841 gcagttgatg ccctggagcg tcaggaaatt cgccgtattc tgctgactcg tccggcggtc
     3901 gaagccggtg agaaactggg cttcctgcct ggcgatttaa gccagaaagt agacccgtat
     3961 ctgcgcccac tgtacgacgc gctgtttgaa atgctgggct ttgagaaagt cgagaaactg
     4021 attgagcgca acgttattga agtcgcgccg ctggcctata tgcgtggtcg tacgctgaac
     4081 gacgcgttta tcattctcga tgagagccag aacactacca tcgaacagat gaagatgttc
     4141 ctgacccgta tcggttttaa ctcaaaagcg gttatcaccg gcgacgtcac acagatcgac
     4201 ctgccgcgta atactaaatc aggcttacgt catgccattg aagtgctggc cgatgtcgaa
     4261 gagatcagct ttaacttctt ccacagcgaa gacgtggttc gtcacccggt ggtggcgcgt
     4321 atcgttaacg cttatgaagc ctgggaagaa gcagaacaaa aacgtaaagc ggcgctggca
     4381 gcagaacgca agcgcgaaga acaggaacaa aaatgagtca ggtgatcctc gatttacaac
     4441 tggcatgtga agataattcc gggttaccgg aagagagcca gtttcaaaca tggctgaatg
     4501 cggtgatccc gcagtttcag gaagagtcgg aagtgactat tcgcgtggtc gataccgccg
     4561 aaagccacag tctgaatctg acctatcgcg gtaaggataa gccgaccaac gtgctctcct
     4621 tcccgtttga agtgccgcct ggcatggaga tgtcgctact gggcgatctg gttatctgcc
     4681 gtcaggtggt tgagaaggaa gctcaggagc aaggcaaacc actggaggcg cactgggcgc
     4741 atatggtggt gcacggcagt ctgcatttgt taggttacga tcacattgaa gatgacgaag
     4801 cagaagaaat ggaagccctc gaaacagaga ttatgcttgc tctgggctat gaggatccgt
     4861 acattgccga gaaagaataa tttgccaggc atttgactgg cagacgtccc cgccgttggt
     4921 gattaacgac ggcgttttga ttaactaaca tgagaaccca tacgacgcca tgagcgacga
     4981 caattcacac agtagtgaca cgataagcaa caagaaggga tttttctccc tgttactcag
     5041 ccaacttttc cacggtgaac cgaaaaaccg tgacgaactg ctggcgctga tccgtgattc
     5101 cgggcagaac gaccttatcg acgaagatac gcgcgatatg ctcgaagggg tgatggacat
     5161 cgcagaccaa cgcgttcgcg acatcatgat cccccgctcc cagatgatta ccctgaaacg
     5221 caaccagacg ctggacgaat gccttgatgt catcatcgag tccgcccact cacgtttccc
     5281 ggtgattagc gaagacaaag atcacattga agggattctg atggcgaaag acttgctgcc
     5341 gtttatgcgc agcgatgctg aagccttcag catggacaaa gtgttacgtc aggcggttgt
     5401 cgttcctgaa agtaagcgcg tagaccggat gctgaaagag tttcgctctc agcgttacca
     5461 catggcgatc gttattgacg aattcggtgg ggtttccggt ctggtgacca ttgaagacat
     5521 cctggaactg attgttggtg agattgaaga cgagtatgac gaagaagatg atatcgactt
     5581 ccgtcagctg agtcgtcata cctggaccgt gcgcgcactg gcttccattg aagacttcaa
     5641 cgaagcgttc ggcacccact ttagcgatga agaagtcgac actatcggtg gtctggtgat
     5701 gcaggcgttt gggcatcttc cggcgcgtgg cgaaactatc gacatcgacg gttaccagtt
     5761 caaagtggcg atggccgata gtcggcgtat tattcaggtt catgtcaaaa tcccggatga
     5821 ctcaccccag ccaaagctgg atgaataaaa ccgaaactgg atagataact acatggcttt
     5881 tgcctcatta attgaacgcc agcgcattcg cctgctgctg gcgttattat tcggtgcctg
     5941 cggaacgctg gccttctctc cttacgacgt ctggcctgcg gcgattattt cgctgatggg
     6001 gcttcaggcg ttgaccttta accgtcgccc actccagtct gccgctattg gcttttgctg
     6061 gggatttggc ctctttggca gcggtattaa ctgggtctat gtcagcatcg cgacctttgg
     6121 cgggatgcct ggcccggtta acatcttcct ggtggtgctg ctggcggcgt atttgtcgct
     6181 gtataccgga ctgtttgctg gcgtgctgtc gcgtctgtgg ccgaaaacca cctggctgcg
     6241 cgtggcgatt gccgcccctg ctctctggca agtgaccgag tttctgcgcg gttgggtgct
     6301 gactggcttc ccgtggttac agttcggcta tagccagatt gatggcccgt taaaagggct
     6361 ggcaccgcta atgggcgtgg aagccattaa cttcctgctg atgatggtta gtggcctgct
     6421 ggcactggcg ttggtcaaac gcaactggcg tccgctggtg gtggccgtcg tgctgtttgc
     6481 cctacccttc ccgctgcgtt acatccaatg gtttaccccg caaccggaga aaaccattca
     6541 ggtttcgatg gttcagggcg atattccgca atcgctgaaa tgggacgaag gccagcttct
     6601 taatacgcta aagatttact acaacgcaac ggcaccgctg atgggcaaat catcgttgat
     6661 tatctggccg gagtcggcga taaccgatct ggaaattaat cagcaaccgt tcctcaaagc
     6721 actggacggt gagttgcgtg ataaaggtag ctcgctggta accgggattg tcgacgcgcg
     6781 tctcaataag cagaaccgct acgataccta caacaccatc atcacgctgg gtaaaggtgc
     6841 accgtacagc tacgaatcag ccgatcgcta taacaaaaac catctggtgc cgtttggcga
     6901 gtttgtcccg ctggagtcga ttctgcgtcc gttagcaccg ttctttgatc tgccgatgtc
     6961 gtcgttcagc cgtgggccat atatccagcc gccgctgtcg gcaaatggta ttgagcttac
     7021 tgcggctatt tgctacgaga tcattctcgg cgagcaagtg cgcgataact tccgcccgga
     7081 taccgactac ctgctgacta tctccaacga tgcgtggttt ggtaaatcta ttggtccatg
     7141 gcaacacttc cagatggcgc gaatgcgtgc gctggagctg gcgcgcccac tgttgcgcag
     7201 caccaacaac ggcattacgg cggtgattgg cccgcagggt gagattcagg cgatgatccc
     7261 gcagttcacc cgcgaggtgt taaccactaa cgtgacgccg accaccggac tcacaccata
     7321 cgcacgtacc ggcaactggc cgctgtgggt gctgacggca ctgtttggtt ttgctgctgt
     7381 gttgatgagt ctgcgtcagc gacgtaaata atcccttctt atcgtgccgg aatacatccc
     7441 atatttcggc acgctcaccg gatgcggcat cagtgcatta tccggataac aatattctcc
     7501 tatccaactt tggcacatct attgctttgt tatacaaggc aaagcttgaa ccagcatcag
     7561 tacaacccgg ttgcaccaca gcaatccacc ggtagcacca aaagagtgca ttttaagaat
     7621 tttgcttcag aatggtgcgg tgcacctcgc aaaaacaaac aataaaacat caatatcttt
     7681 acatttagcc aaactaaatg ttacctaaca ggcacaacac tgcacaataa agttgcagac
     7741 gataacaaca caaacactca caacgggtat ccatgcgttc ttaacgcaga agataaagga
     7801 gttggatatg caattacgta aacctgccac agcaatcctc gccctggcgc tttccgcagg
     7861 actggcacag gcagatgacg ccgccccggc agcaggcagt acgctggaca aaatcgccaa
     7921 aaacggtgtg attgtcgtcg gtcaccgtga atcttcagta cctttctctt attacgacaa
     7981 tcagcaaaaa gtggtgggtt actcgcagga ttactccaac gccattgttg aagcagtgaa
     8041 aaagaaactc aacaaaccgg acttgcaggt aaaactgatt ccgattacct cacaaaaccg
     8101 tattccactg ctgcaaaacg gcactttcga ttttgaatgt ggttctacca ccaacaacgt
     8161 cgaacgccaa aaacaggcgg ctttctctga cactattttc gtggtcggta cgcgcctgtt
     8221 gaccaaaaag ggcggcgata tcaaagattt tgccgacctg aaaggcaaag ccgtagtcgt
     8281 cacttccggc actacctctg aagttttgct caacaaactg aatgaagagc aaaaaatgaa
     8341 tatgcgcatc atcagcgcca aagatcacgg tgactctttc cgcaccctgg aaagcggtcg
     8401 tgccgttgcc tttatgatgg atgacgctct gctggccggt gaacgtgcga aagcgaagaa
     8461 accagacaac tgggatatcg tcggcaagcc gcagtctcag gaagcctacg gttgcatgtt
     8521 gcgtaaagac gatccgcagt tcaaaaagct gatggatgac actatcgctc aggtgcagac
     8581 ctccggtgaa gcggaaaaat ggtttgataa atggttcaaa aatccaattc cgccgaaaaa
     8641 cctgaacatg aatttcgaac tgtcagacga aatgaaagca ctgttcaaag aaccgaatga
     8701 caaggcactg aactaattac aagaaccagg ggcggaaatt ccagccctct cgattgttac
     8761 gtagcacgga cagactatac gcctgatggt cgttccccat cgggcctgaa aaccgcaata
     8821 cgctgggtaa caatcttcga gggtagcagt taacgctgct accctctttt ttctggagta
     8881 gatttatgtc tatagactgg aactggggta tttttttaca acaagccccg ttcggcaata
     8941 ccacctatct tggttgggtc tggagtggtt ttcaggtgac gatcgctttg tcgatctgcg
     9001 cctggattat cgctttcctc gtcggttcat ttttcggcat tttacgtacc gttccaaacc
     9061 gttttctctc cggtctgggt acgttgtatg tcgaactgtt ccgcaacgtg ccgttgattg
     9121 tgcaattctt tacctggtat ctggtgatcc cggagctgct gccggagaaa atcggcatgt
     9181 ggtttaaggc cgagctggat cccaatattc agtttttcct ttcatccatg ctctgcctgg
     9241 ggctgtttac cgccgcccgt gtttgcgaac aggtacgcgc cgctattcag tcgctaccgc
     9301 gtgggcagaa gaatgccgcg ctggcgatgg gcctgacgct accgcagact tatcgatacg
     9361 tactgctgcc taatgcttat cgcgttatcg tcccgccgat gacttccgag atgatgaacc
     9421 tggtgaaaaa ctccgccatc gcctcgacca tcggtctggt ggatatggcg gcgcaggcgg
     9481 gtaaattgct cgactactcg gctcacgcct gggagtcgtt taccgccatc acgcttgcgt
     9541 atgtgttgat taacgctttt atcatgctgg tgatgacgct ggttgaacgt aaagttcgcc
     9601 tgcccggcaa catggggggc aaataatgta cgagtttgac tggagttcca ttgttccttc
     9661 cctgccatat ctgctcgacg ggctggtgat caccctgaaa atcaccgtca cggcagtcgt
     9721 gattggtatt ttgtggggta ccatgctggc ggtgatgcgc ttgtccagct tcgcgcctgt
     9781 ttcgtggttt gccaaagcct acgttaacgt attccgctcg attcctttag tcatggtttt
     9841 gctgtggttt tacttgattg tgccgggatt tctgcaaaat gtgctgggat tatcgccaaa
     9901 aaatgatatt cgtttaattt cggcgatggt ggcgttttcg atgtttgaag cggcctacta
     9961 ttcagagatt atccgtgccg gtattcaaag tatttcccgt ggtcagtcga gcgccgcgct
    10021 ggcgttgggg atgactcact ggcagtcgat gaaactgatt attctgccgc aggctttccg
    10081 cgcgatggtg ccgctgctgc tcactcaggg catcgtactg ttccaggata cctcgctggt
    10141 gtatgtgtta tctctggccg atttcttccg taccgcctca accattggtg agcgtgatgg
    10201 tacgcaggtc gagatgatcc tgtttgccgg atttgtttat ttcgttatta gccttagcgc
    10261 gtcgttgttg gtcagctact tgaaaagaag gacagcataa tgattaccct gaaaaatgtt
    10321 tcaaaatggt atggtcactt tcaggtgctg accgactgct caaccgaagt gaaaaaaggc
    10381 gaagtggtgg tggtttgcgg cccgtctggt tccggcaaat caacgctgat taaaaccgtc
    10441 aacggcctcg aaccagtgca gcaaggtgaa atcaccgtcg atggtatcgt ggttaacgac
    10501 aagaaaaccg atctggcaaa gctgcgttcc cgcgtcggga tggtattcca gcatttcgag
    10561 ctgttccctc atctgtcgat tatcgaaaac ctgaccctgg cgcaggtgaa agtgcttaaa
    10621 cgcgataaag cgcccgctcg tgaaaaagca ttgaaattgc tagagcgcgt gggactttcc
    10681 gcacatgcca ataaatttcc ggcgcagctt tccggcggtc agcagcagcg tgtggcaatc
    10741 gctcgcgcat tgtgtatgga tcctattgcg atgctgttcg acgaaccgac atcggcgctg
    10801 gatccggaga tgatcaacga agtgctggac gtgatggtgg aactggcgaa cgaaggaatg
    10861 accatgatgg tggtgaccca cgaaatgggc tttgcccgca aagtggcgaa tcgggtgatc
    10921 tttatggacg agggtaaaat tgtcgaagac tcgccgaaag acgctttctt cgatgatccg
    10981 aaatcggacc gcgcaaaaga cttcctcgcg aaaatcctgc attaatcact ctggcgcgca
    11041 tacccaattg cgcgccatct cacgctttat cgtttcgccc ctcgtatctg ttacatcctg
    11101 gcgttaccct tgtcgcaaag aagcacacaa caaggagcaa caatggcact gccaattctg
    11161 ttagattgcg acccaggtca tgatgacgct atcgcaatag ttctcgccct cgcctcacca
    11221 gagcttgatg tcaaagcaat tacgtcttcc gccggaaacc agacaccaga aaaaacctta
    11281 cgcaatgttc tgcgtatgct gaccttgctt aatcgcaccg atattccggt ggcaggcggc
    11341 gcggtaaaac cgttaatgcg tgagttgatt atcgcggaca atgtgcacgg cgaaagcggt
    11401 ctcgacggcc cggcattacc ggaaccgacg ttcgcaccgc aaaactgtac ggcggtggag
    11461 ctgatggcga aaacgctgcg cgaaagtgcg gaacctgtca cgattgtttc taccggaccg
    11521 caaactaacg ttgccttgct gctcaatagc catccggaac tgcatagcaa aattgcccgt
    11581 atcgtgatta tgggcggcgc aatggggctt ggtaactgga cgcctgcggc tgaatttaat
    11641 atttacgttg acccggaagc ggcagaaatt gtcttccagt cagggatccc ggtggtgatg
    11701 gccggtctgg atgttactca taaagcgcaa atccacgttg aagacaccga gcgtttccgc
    11761 gcgattggca accctgtttc aaccattgtt gccgaactgc tggatttctt cctcgaatat
    11821 cataaagacg aaaaatgggg ctttgtcggc gcaccgctgc atgacccatg caccatcgcc
    11881 tggctgttga aacccgagtt gtttaccact gttgagcgct gggttggcgt ggaaacacag
    11941 gggaaatata cccagggtat gacggttgtt gattattatt atctgactgg caataaaccg
    12001 aatgccactg taattgtcga tgttgatcgt cagggctttg ttgatttact ggctgatcgt
    12061 ctgaaatttt acgcttaaca ctcgtcattg attaagatca ccacgcgtta cctgggtggt
    12121 cttaatctat ttatctcttt ttctttcaag atataaatgc aatggataat gcagaactcg
    12181 ccataggtat cgatctcggc actaccaata gtttaattgc cgtctggaaa gacggtgccg
    12241 cgcaattaat tccaaataag ttcggtgaat atttaacacc atccataatt agcatggatg
    12301 aaaataatca tattttagtc ggaaaaccgg ctgtatcacg gcgtacttcg catccggata
    12361 aaacggcagc gttatttaaa cgtgcaatgg gcagtaatac caactggcgg ttaggcagcg
    12421 acacatttaa cgcgccagaa ctgtcctctt tggtattacg ctcattaaaa gaagatgccg
    12481 aagaatttct gcaacgtccg attaaagatg tggtgatctc cgttccggct tatttcagcg
    12541 atgaacaacg caagcatacc cgtttagcag cggacttagc cgggttaaat gcggtacgct
    12601 taattaatga acccacagca gctgcgatgg cgtatggcct gcatacccaa caaaataccc
    12661 gttcgctggt gtttgatctc ggtggcggca cgtttgacgt tacggtgctt gagtacgcca
    12721 cgccggtgat tgaagttcac gcctccgctg gcgacaactt cctcggtggc gaagatttta
    12781 cccatatgtt ggtcgatgac gttttaaagc gcgcggatgt cacaaggacc acgcttaacg
    12841 agagtgaact ggcagccttg tacgcctgtg tggaagcggc aaaatgtagc aatcaatcgc
    12901 caatgcacat tcgctggcag taccaggatg aaacgcggga atgcgaattt tacgagaacg
    12961 aactggaaga tttgtggttg ccgctgctca atcgcttgcg agtgccgatt gaacaggcgt
    13021 tgcgcgatgc gcgtctgaag ccgagtcaaa tcgacagtct ggtgctggtt ggcggcgcgt
    13081 cacaaatgcc gctggtgcag cgaatcgccg tgcgtctgtt tggcaaatta ccgtatcaaa
    13141 gttacgatcc gagcaccatt gtcgcgctgg gcgcagcaat ccaggccgcc tgccgcttac
    13201 gcagtgaaga tattgaagaa gtgatcctca ctgatatttg cccttactcg ttgggcgttg
    13261 aggttaaccg ccagggcgtt tccggcattt tctcgccgat tattgaacga aacaccactg
    13321 tgcccgtgtc gcgtgtagaa acttattcaa ccatgcaccc ggaacaggat tcaattacgg
    13381 ttaacgtcta tcagggagaa aaccacaaag ttaaaaacaa cattctggtg gaatccttcg
    13441 atgtgccgtt gaagaaaacc ggggcttatc agtcgattga tattcgcttt agttatgata
    13501 tcaacgggtt gcttgaagtt gacgtgcttc tggaagacgg cagcgttaag tccagagtga
    13561 ttaaccacag cccggtaaca ttgagcgcgc agcagattga agagagtcgg acgcggttat
    13621 ccgcattgaa aatttatccg cgcgatatgc tcatcaatcg cacctttaaa gccaaactgg
    13681 aagagttgtg ggcgcgggcg ctgggtgacg agcgagaaga gattggccgg gtgatcaccg
    13741 attttgatgc ggcgctgcag tcaaacgata tggcccgcgt cgatgaggtc aggcggcggg
    13801 cgagcgttta tttagccatt gagacctcat aatcacggct gccggatgcg gcgtaaatgc
    13861 tgcatccggc atcggcgcaa aaaacacatc gtcaactaaa caacatcgcg attttgccga
    13921 gagcataacc tataaccagc accgttaaac tgattttgat aatgctggta atgctctgtg
    13981 gtttttcacc ctcatcattt ttttcttcat ggttttcagg tgttggctca tcgacatctt
    14041 catcctccgg ctcgagtttt actttcgtca acagcatccg ggtgataatg gcctctaatg
    14101 gatgagcatc tccgccttcc agccttgtat taagacgggc cagcatctct tgtttcggaa
    14161 acgcttcaag tactacctgt tccgctaccc gatacaaatc cagtaacggt gaatcttctt
    14221 ctgtctcgct accgactctc gatttccacc acgcccatgc tttgcgtttg aagaataacg
    14281 actcaataaa cgattgacct aacggcgata attttccgct atgtaaaatt tccgcccata
    14341 acagccgcgt ccgggcgctc tcaccaggaa catatttcag cggttgacct tgcgcatcta
    14401 ccatgctccg cgcttcgata accatcacta ataacgcgaa ataatccggg caatgacgct
    14461 tgctccactg caacaacaaa ttatcagctt gtgtagatgg gtaactttcc cagtacgcgc
    14521 cgagatcggc aagcaggctt tccccctcgc cgcagtacaa acgttgtgca cactggtaat
    14581 accgggcatc ttcgctttct ggttcttccg cttgccactg tttggctaca ggaatgagtt
    14641 ccaccatacc tgtttgcacc gagctgtacc agcgcaacag tttgcgatgt aactttgcat
    14701 catcaggaat gaaccacggt ccttccatcg ccaaaaacgc agtgacatag tcaggatggt
    14761 aacggcagca acgttccagc gcgaagtaat attcaacagt ctggttctgt aacgcgacgg
    14821 gatattccag aaggctaagg aaatcgaaac aatcaccagc ctgaatggcc tcaagaaaag
    14881 cctccctttc ttcctcatcc attccttcgc tgtcattaaa cgaatgccag ttcaaacgct
    14941 ctgccaacaa agaagcacaa tttaatgaca gatatcgcgc ttctatagcg attgcacaca
    15001 gcggccagcg taattgatcg acatcctcca tgttccaggt atttaattgc tgaataaatt
    15061 tctgccaggc ggagggctga aaacgatcac tttctgaatc cagtaatgtc ctgaatgcac
    15121 gtagtatttc atgttcagcg gtggcatctt tttcttcatc atctgcttcc tcgacaggat
    15181 ttaccgccag tcgcagcgcc tcttcatagg cctggcgtaa tgctttaaac ccctgcggat
    15241 cggtttcggg atgacacaac ggtaagcgag caagataagc ctggcggata atgtctattt
    15301 gcgtcgtgct ttcaatttcg aggatttgcc aacatgtttt cataggtcgc tcacatcctt
    15361 aagcgttacc gggcattcag gctggacagt taatggctgc cagggaagcg tggcatagaa
    15421 acggtcgctt cccgctgcca gctcttcgaa catccgactc ccggcgctat cgtatttgta
    15481 cagggttagc gcctcgcgta gcgaagcgtt gtcttcgcac tgagaagatt gccagtagag
    15541 acgccctagt gaataggcag aaaaatagtg catccagctg tgatagtaat ggtgagctcg
    15601 tgcatagacg cgcgattgca gtcataaact ctcttcttcc gtcagccagt tgttgagcac
    15661 accgacccgt gacagaaacc ccattcgaac ataatcccag gctttgattc ccccttctcc
    15721 acagcacata gcggtttggg caacgaattg ggtataaatc aaccctctct ccgagccacc
    15781 agcaattaat gctttccact cttcagggga agagcgttgc catgtcagat ataagccagc
    15841 taaatcattg gcatgaccat catcagtcat ctgataaata gtctgaatta aatcatcacg
    15901 attatttatt ccccaggatg atgagagatc gaattctttt atcccggtat acatttttgg
    15961 cgagtcatgc gcgggtttgc aactctgatt aagtatttcc atgggcgcag aaagcgcaaa
    16021 aagaagatat tgttcttcct tattcatttt ttaataatac cttaagtgca ttaactgact
    16081 acttttttat tattgatgag tataaacttg acgctttcaa aataaaaaga aaatcgaagc
    16141 attcacacat gaataaaaaa ttaatgtata tattcgcaat ttttatagtt gcagcaatta
    16201 cctgtattag ccaacccaag aaaacgacgt tgcgtgataa agccatggtg aattatgcct
    16261 ttgattattt aagatcacca ggcagtctac cgttaaccac ggcagccacg gagctttccg
    16321 cgattcatgg tcactcaaca tcgcaatatc gccttggcga attttatctt catggtagcg
    16381 acggtaaacc actggattat acacaggcga gatactggta tgagcaatca gcagaacagg
    16441 aaaatccgcg cgcacaaagc aaactggggt ggatctacct gaaaggcctg ggagtcaaac
    16501 ccgatacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg
    16561 ctcaatatac tttgggtttg atctacagaa atggcactgg tattaatgtt aaccattatg
    16621 aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac
    16681 ttgccggact tcccgcacat taacgtcgac tgtcacccga agagatggag tattttccct
    16741 atcaaaccgg caaagataaa aatgtagaaa ataattttga tgacgcccag acagcccagc
    16801 ccgccatcgt caacgggcat tggctgcggt tgcggctccg gctcgacggt ttcctgaccg
    16861 gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag tggacaacga
    16921 tcttctgcat caaggcggct cacaatcggc attaatgatt cttctgtcgg ttggctggcc
    16981 tgcactaatt gtttggcaac taaatataat ggccataagg gatgttctgt atcacgcgtg
    17041 agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga acgccccagc
    17101 gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg
    17161 ccggggacat aaagtagcgg ttttccctga tcgttaacca gatcacgcgc ttcaatcatc
    17221 atcaccacta atgggtaata atcgacccga tgctgacggc accattgcaa cattaaagca
    17281 tccgcctggg tggagggata ctcgcgccag tagtaacaaa gttctgccag caggctttcg
    17341 ccttctccac aatagacgcg ttgtgcgtat tcataataac gcgcatcttc attatcaggt
    17401 tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg ggattcccag gcggcttgcg
    17461 ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat cactgcgcga
    17521 tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg agcgaacatt
    17581 tggtaaaaat cgatggtgtc attttgcacg gcaacgggca gatgcagaat ggtctggaag
    17641 ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc ttcgtctatt
    17701 tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac cacgcactcg
    17761 aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa ttcatcaatc
    17821 tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga gggcagaaaa
    17881 cgttcgctat cagaggcaag taacgcgcga aaggcgagca gaatttcatg ttctgctacc
    17941 tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg tagcgcctcc
    18001 tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg aaaggacggt
    18061 aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc tatatcgagg
    18121 atcttccagc aatttttcat aagtcgctca tatccttaag cgtttccggg catgtggggt
    18181 agtcagccag tggttgccag gacaaggtag ggtaatacgc atcatcactg gcaaataatt
    18241 gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat tcgcgtaata
    18301 aaggcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta taaccggcaa
    18361 agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc gactggatcc
    18421 atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt aaaaaaccca
    18481 tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct gtgctggcga
    18541 caaattgcgc ataagcctga ccttgctcat ttaattcagc aagatagtca cgccattcac
    18601 atggagaata acgaaaccag cggtgataaa accctgccaa ccgagcggca tgaccgttct
    18661 cgatcattcg gtgaattgtg cgcagcaggt catcacggtc atcaattccc catgaggaag
    18721 aaaggtcaaa aaagcatgta cctaaataca ttttggggga taaatgcgaa ggtaaacagc
    18781 attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc gaccccatgt
    18841 ccatataatt tatccttaaa cattcattta atgaatgtaa actacatttc gagactcagt
    18901 tatttatact tttctgacgc aacataatac gcataaaata aggcttacgc aaaaatggaa
    18961 cacataaaaa tataatatac tttatctaca accataagga aatgataatg atttttacgt
    19021 caagttgctg cgataattta tcaatagatg agattatcga acgtgctgaa aaaggcgatt
    19081 gcgaggctca atatattgtt gggttttatt ataatcgcga tagcgcaatt gactctccgg
    19141 acgacgaaaa ggccttttac tggctgaagc tggccgctga gcaaggtcat tgtgaagcac
    19201 agtattcctt agggcaaaag tataccgagg ataaaagccg tcataaagat aatgagcaag
    19261 ccatcttctg gctgaaaaaa gctgccctac aaggccatac tttcgcttcc aacgcccttg
    19321 gctggatact ggatcgtgga gaagacccca actataaaaa agaagcagtt acctggtatc
    19381 agatagccgc ggagagcgga atgtcttatg cgcaaaataa tcttggatgg atgtacagaa
    19441 atggcaacgg agtcgcacaa gactatacgc tggcattttt ctggtacaaa caagctgcat
    19501 tacaaggcca tagttacgca caagacaatc tggccgatct ttataaagac ggagaaggcg
    19561 ttgctcaaaa caagacactc gccgcattct ggtatttgaa aagcgcacag cagggtaatc
    19621 ggcacgccca gtttcaaatt gcatgggatt ataacgctgg cgaaggggtg gaccaggact
    19681 ataagcaagc gatgtactgg tatctgaagg ctgccgctca ggaaagcgtc ggcgcttacg
    19741 tcaacatcgg ttatatgtat aaacacggac aaggcgttga gaaagattat caggctgcct
    19801 ttgaatggtt tacgaaagcc gctgaatgca atgacgccac tgcctggtat aacctggcca
    19861 ttatgtatca ctacggagaa ggaagacctg tcgatctccg acaggctctc gacctgtatc
    19921 gtaaagttca gtcatccgga accagagatg tcagtcagga aattcgtgag actgaagatt
    19981 tactgtagaa ataaaaacaa gggccgggga atttcattct gcccatattc tctttattgc
    20041 tgtgcgaaat cagactatta cggctcaaac gggcgtctct ggaactggtc ataaccacat
    20101 ttcgggcata gcgtcagcac ttccggtgtg tagatcggga gatggaagtg acatttctcg
    20161 cagaccagat ttcccagccc gaccacttct ccgctgtgat aaaccccatg atgattgagg
    20221 tcctggaaaa cttcgcgcca ttcaagctgc gttttatcgg tgatgtctgc cagctcctgc
    20281 cacaagcttt ctttaatcac ccgcataaag acgctgtcag attcttcttt caggctctct
    20341 tcatagctca tggcgaactc ttccaggtca cgtctgacag ctcgcgtcag ctcatcgatc
    20401 tcggttcgcg ttaactcccc tgtttttatt acgcgctcgc gcgcctgttc caccagtgcg
    20461 tcgatatcac gttcgccatt gcgcaggcgt tcgttcagtg acgcgaccag ttcacggtaa
    20521 tattgagcaa ccttgttcat cgtttcatct cccgggtcgt taactgacta taataataga
    20581 cgttctttgt tccttgcttt gctaatacgc ccacaaactc tgtaaattaa tgtacgaata
    20641 atccgggcat catttcaacg tcgagtgcgc gaaaggctgt tttgacgcgg acgtttgggc
    20701 tatgctatgc ggatctgaaa aaccacctca acgctacatt tgtagccgta ttgaaaacag
    20761 gaccactggc tgccatgcaa gagcaatacc gcccggaaga gatagaatcc aaagtacagc
    20821 ttcattggga tgagaagcgc acatttgaag taaccgaaga cgagagcaaa gagaagtatt
    20881 actgcctgtc tatgcttccc tatccttctg gtcgactaca catgggccac gtacgtaact
    20941 acaccatcgg tgacgtgatc gcccgttacc agcgcatgct gggcaaaaac gttctgcagc
    21001 cgatcggctg ggacgcgttt ggtctgcctg cggaaggcgc ggcggtgaaa aacaacaccg
    21061 ctccggcacc gtggacgtac gacaacatcg cgtatatgaa aaaccagctc aaaatgctgg
    21121 gctttggtta tgactggagc cgcgagctgg caacctgtac gccggaatac taccgttggg
    21181 aacagaaatt cttcaccgag ctgtataaaa aaggcctggt atataaaaag acttctgcgg
    21241 tcaactggtg cccgaacgac cagaccgtac tggcgaacga acaagttatc gacggctgct
    21301 gctggcgctg cgataccaaa gttgaacgta aagagatccc gcagtggttt atcaaaatca
    21361 ctgcttacgc tgacgagctg ctcaacgatc tggataaact ggatcactgg ccagacaccg
    21421 ttaaaaccat gcagcgtaac tggatcggtc gttccgaagg cgtggagatc accttcaacg
    21481 ttaacgacta tgacaacacg ctgaccgttt acactacccg cccggacacc tttatgggtt
    21541 gtacctacct ggcggtagct gcgggtcatc cgctggcgca gaaagcggcg gaaaataatc
    21601 ctgaactggc ggcctttatt gacgaatgcc gtaacaccaa agttgccgaa gctgaaatgg
    21661 cgacgatgga gaaaaaaggc gtcgatactg gctttaaagc tgttcaccca ttaacgggcg
    21721 aagaaattcc cgtttgggca gcaaacttcg tattgatgga gtacggcacg ggcgcagtta
    21781 tggcggttcc gggtcacgac cagcgcgact acgagtttgc ctctaaatac ggcctgaaca
    21841 tcaaaccggt tatcctagcg gctgacggct ctgagccaga tctttctcag caagccctga
    21901 ctgaaaaagg cgtgctgttc aactctggcg agttcaacgg tcttgaccat gaagcggcct
    21961 tcaacgccat cgccgataaa ctgactgcga tgggcgttgg cgagcgtaaa gtgaactacc
    22021 gcctgcgcga ctggggtgtt tctcgtcagc gttactgggg cgcgccgatt ccgatggtga
    22081 cgctggaaga cggtaccgta atgccaaccc cggacgacca gctgccggtg atcctgccgg
    22141 aagatgtggt gatggacggc attaccagcc cgattaaagc agatccggag tgggcaaaaa
    22201 ctaccgttaa cggtatgcca gcgctgcgtg aaaccgacac tttcgacacc tttatggagt
    22261 cctcctggta ctatgcgcgc tacacttgcc cagagtacaa agaaggtatg ctggattccg
    22321 aagcggctaa ctactggctg ccggtggata tctacattgg tggtatcgaa cacgccatta
    22381 tgcacctgct ctacttccgc ttcttccaca aactgatgcg tgatgcaggc atggtgaact
    22441 ctgacgaacc agcgaaacag ttgctgtgtc agggtatggt gctggcagat gccttctact
    22501 atgttggcga aaacggcgaa cgtaactggg tttccccggt tgatgctatc gttgaacgtg
    22561 acgagaaagg ccgtatcgtg aaagcgaaag atgcggcagg ccatgaactg gtttataccg
    22621 gcatgagcaa aatgtccaag tcgaagaaca acggtatcga cccgcaggtg atggttgaac
    22681 gttacggcgc ggacaccgtt cgtctgttta tgatgtttgc ttctccggct gatatgactc
    22741 tcgaatggca ggaatccggt gtggaagggg ctaaccgctt cctgaaacgt gtctggaaac
    22801 tggtgtacga gcacacagca aaaggtgatg ttgcggcact gaacgttgat gcgctgactg
    22861 aagatcagaa agcgctgcgt cgcgatgtgc ataaaactat cgctaaagtg accgatgata
    22921 tcggccgtcg tcagaccttc aacaccgcaa ttgcggcgat tatggagctg atgaacaaac
    22981 tggcgaaagc accgaccgat ggcgagcagg atcgcgctct gatgcaggaa gcactgctgg
    23041 ccgttgtccg tatgctcaac ccgttcaccc cgcacatctg cttcacgctg tggcaggaac
    23101 tcaaaggcga aggcgatatc gacaacgcgc cgtggccggt tgctgacgaa aaagcgatgg
    23161 tggaagactc cacgctggtc gtggtgcagg ttaacggtaa agtccgtgcc aaaatcaccg
    23221 ttccggtgga cgcaacggaa gaacaggttc gcgaacgtgc tggccaggaa catctggtag
    23281 caaaatatct tgatggcgtt actgtacgta aagtgattta cgtaccaggt aaactcctca
    23341 atctggtcgt tggctaagcg cgggaggaag cgtgcgatat ctggcaacat tgttgttatc
    23401 tctggcggtg ttaatcaccg ccgggtgtgg ctggcatctg cgtgacacca cgcaggttcc
    23461 ttccactatg aaggtcatga tcctggactc aggcgatccg aacgggccat taagccgtgc
    23521 ggtgcgtaac cagttacgtc tgaatggtgt cgagttgctt gataaagaaa ccacgcgtaa
    23581 ggacgttcca tccctgcgtt tgggtaaagt gagcatcgcg aaagataccg catcggtatt
    23641 ccgtaacggt caaacagcag agtatcagat gatcatgacg gtgaatgcga ccgtgttgat
    23701 ccccggccgt gatatctacc cgattaacgc caaagtcttc cgttcattct tcgataaccc
    23761 gcaaatggcg ttagcgaaag ataacgaaca agacatgatc gtaaaagaga tgtacgaccg
    23821 tgctgccgaa cagctgattc gtaagctgcc aagcatccgt gctgcggata ttcgttccga
    23881 cgaagaacag acgtcgacca caacggatac tccggcaacg cctgcacgcg tctccaccac
    23941 gctgggtaac tgatgattcg gttgtacccg gaacaactcc gcgcgcagct caatgaaggg
    24001 ctgcgcgcgg cgtatctttt acttggtaac gatcctctgt tattgcagga aagccaggac
    24061 gctgttcgcc aggtagctgc ggcacaagga ttcgaagaac accacacttt ttccattgat
    24121 cccaacactg actggaatgc gatcttttcg ttatgccagg ctatgagtct gtttgccagt
    24181 cgacaaacgc tattgctgtt gttaccagaa aacggaccga atgcggcgat caatgaacaa
    24241 cttctcacac tcaccggact tctgcatgac gacctgctgt tgatcgtccg cggtaataaa
    24301 ttaagcaaag cgcaagaaaa tgccgcctgg tttactgctc ttgcgaatcg cagcgtgcag
    24361 gtgacctgtc agacaccgga gcaggctcag cttccccgct gggttgctgt gcgcgcaaaa
    24421 cagctcaact tagaactgga tgacgcggcg aatcaggtgc tctgctactg ttatgaaggt
    24481 aacctgctgg cgctggctca ggcactggag cgtttatcgc tgctctggcc agacggcaaa
    24541 ctgacattac cgcgcgttga acaggcggtg aatgatgccg cgcatttcac cccttttcat
    24601 tgggttgatg ctttgttgat gggaaaaagt aagcgcgcgt tgcatattct tcagcaactg
    24661 cgtctggaag gcagcgagcc ggttattttg ttgcgcacat tacaacgtga actgttgtta
    24721 ctggttaacc tgaaacgcca gtctgcccat acgccactgc gtgcgttgtt tgataagcat
    24781 cgggtatggc agaaccgccg gggcatgatg ggcgaggcgt taaatcgctt aagtcagccg
    24841 cagttacgtc aggccgtgca actcctgaca cgaacggaac tcaccctcaa acaagattac
    24901 ggtcagtcag tgtgggcaga gctggaaggg ttatctcttc tgttgtgcca taaacccctg
    24961 gcggacgtat ttatcgacgg ttgatatgaa atctttacag gctctgtttg gcggcacctt
    25021 tgatccggtg cactatggtc atctaaaacc cgtggaaacg ctggcgaatt tgattggtct
    25081 gacgcgggtc acaatcatcc ctaataatgt tcctccgcat cgtccccagc cggaagcgaa
    25141 cagcgtgcag cgtaaacaca tgcttgaact ggcgattgcc gacaagccat tatttactct
    25201 tgatgaacgc gagctaaagc gcaatgcccc ctcttacact gcgcaaacac tgaaagagtg
    25261 gcggcaggaa caaggaccgg acgtgccgct ggcgtttatt attggtcagg attcattgct
    25321 gacctttccg acctggtacg aatacgaaac gatactcgac aatgcacatt tgatcgtctg
    25381 tcggcgtcca ggttacccac ttgaaatggc gcaaccgcaa taccagcaat ggctggaaga
    25441 tcatttgaca cataacccgg aagatcttca ccttcagcct gccggtaaaa tttatctggc
    25501 tgaaacgccg tggtttaaca tctcggcgac catcatccgc gaacgtttgc aaaacggtga
    25561 atcgtgtgag gatttattgc cggaaccggt attgacttac attaaccaac aaggcttgta
    25621 tcgctgatac ctgtcgttac ggagggcatg atgcgactgt ggttaattcg tcatggcgaa
    25681 acacaagcga atgtcgatgg tctgtacagc ggtcatgcgc ccacacccct gaccgcacgc
    25741 ggtattgagc aagcgcaaaa tctgcataca ctactagatg atgtttcctt tgacctggtt
    25801 ttatgcagtg aactggaacg ggcacagcat accgcgcgac tggttctcag tgaccgccag
    25861 ctccccgttc atatcatccc tgaactcaac gaaatgtttt ttggcgactg ggagatgcga
    25921 catcatcgcg acctcatgca agaagatgcc gaaaactata gcgcgtggtg caatgactgg
    25981 cagcatgcta tccccacgaa cggtgaagga tttcaggcat tttcgcaacg tgtggaacgc
    26041 tttatcgcaa ggcttagtga atatcagcac tatcagaata ttttaatcgt cagccatcag
    26101 ggcgtactga gtctgttaat tgctcgttta attggcatgc ctgccgagtc tatgtggcat
    26161 tttcgcgttg actaggggtg ttggagcgcc attgatatca accaaaaatt cgcgacgcta
    26221 cgcgtcctca atagccgtgc catcggggtc gagaatgcat gacttttctg tttttttacg
    26281 ggtaagccgc aacgaccatt gacagccccg ggcaggctga tattctccgc agccagactt
    26341 tttccgccag acacgacttt gtagaaattg ttttacaaaa atggcgatgc aatctgcggc
    26401 gcggggtggg atgatagccc actttcgaaa gccgattcgg cgacaattgt cccgaaatcg
    26461 cctctggttc aggtatactg acagaccatt tttatctatt tgattcaccc agggggaaaa
    26521 cttgcagggt aaagcactcc aggattttgt tatcgacaaa attgatgacc tcaaaggtca
    26581 ggacatcatc gccttagacg ttcaaggcaa atccagcatc accgactgca tgatcatctg
    26641 tacgggtacg tccagccgtc atgttatgtc cattgctgac cacgttgtgc aggagtctcg
    26701 cgcagcgggc ctgttaccgc tcggcgtaga aggtgaaaac agcgccgact ggattgtcgt
    26761 ggatttgggc gatgtgattg tccatgtcat gcaggaagag agccgtcgcc tgtatgaact
    26821 ggaaaaactc tggagttaat gcgtgaagct gcaacttgtc gccgtgggaa cgaaaatgcc
    26881 ggactgggta caaaccggtt ttaccgagta cctgcgtcgt tttccgaaag atatgccctt
    26941 cgagctgatt gaaattccgg ccggcaaacg cggcaagaat gcggacatca agcgcatact
    27001 cgacaaagag ggtgagcaga tgttggcggc cgcaggcaaa aaccgcattg tcaccctcga
    27061 tattccaggc aagccctggg atacgccgca gttagccgct gagctggaac gctggaagct
    27121 ggatggtcgc gacgtaagtc tactgattgg cgggcctgaa gggttgtcgc ctgcctgtaa
    27181 agcggcggct gagcagagct ggtcgctgtc ggcgcttacc ctcccccatc cgctggttcg
    27241 cgtgctggtc gcagagagtc tgtaccgggc gtggagcatc accaccaacc atccttatca
    27301 ccgtgagtga taagggagct ttgagtagaa aacgcagcgg atgaaactac agaactcttt
    27361 tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg gcgctggtcg cctttttggg
    27421 gattttgctg ctgaccggcg tgcttatcgc caacctgtat aatctgcaaa ttgttcgctt
    27481 taccgactac cagacccgct ctaatgaaaa ccgcattaag ctggtgccta tcgcgcccag
    27541 ccgcggcatt atctacgacc gtaacggtat ccctctggcc ctcaaccgca ctatctacca
    27601 gatagaaatg atgccggaga aaatcgataa cgtgcagcaa acgctggacg ctttgcgcag
    27661 cgtggtagat ctgaccgatg acgatattgc tgcattccga aaagagcgcg cacgttcaca
    27721 ccgtttcacc tctattccgg tgaaaactaa cctgaccgaa gtacaagtag ctcgctttgc
    27781 cgtcaatcag taccgttttc cgggtgtcga agttaaaggc tataaacgtc gttactatcc
    27841 ttacggttcg gcgttgaccc acgtcatcgg ctatgtgtcg aaaatcaacg ataaagacgt
    27901 cgaacgcctg aataatgacg gcaaactggc caactatgcg gcaacgcatg atattggtaa
    27961 gctgggcatt gagcgttact atgaagacgt gctgcacggt cagaccggtt atgaagaggt
    28021 tgaagttaac aaccgtgggc gtgttatccg ccagttaaaa gaagtaccac cgcaagccgg
    28081 acacgatatt tacctgacgc tggatctcaa actccagcaa tatattgaaa cgctgctggc
    28141 gggtagccgc gcagctgtgg ttgttaccga tccgcgtaca ggtggggtgc tggcgctggt
    28201 ttccacgcct agttatgacc caaacctgtt tgttgacggt atctccagca aagattattc
    28261 cgccttgctg aacgacccga atacaccgct ggtgaaccgc gccacacagg gggtttatcc
    28321 tcccgcgtct acagttaaac cctatgtggc ggtttcggca ttaagcgccg gggtgatcac
    28381 gcgcaatacg acgctgtttg acccaggctg gtggcaacta ccagggtcgg aaaaacgtta
    28441 tcgtgactgg aaaaaatggg gccacgggcg tctgaatgtc acaagatcgc tggaagaatc
    28501 tgcggatacc ttcttctatc aggtggccta cgatatgggg atcgatcgcc tctccgaatg
    28561 gatgggtaaa ttcggttatg gtcattacac cggtatcgac ctggcggaag aacgttccgg
    28621 caacatgcct acccgcgaat ggaaacagaa acgctttaaa aaacagtggt atcagggtga
    28681 caccattccg gttggtatcg gccagggtta ctggacagcg accccaatcc agatgagtaa
    28741 ggcactgatg atcctgatta atgacggtat cgtgaaggtt ccgcatttgc tgatgagcac
    28801 cgccgaagac ggcaaacagg tgccatgggt acaaccgcat gaaccgcccg tcggcgatat
    28861 tcattccggt tactgggagc tggcgaaaga cggtatgtac ggtgttgcta accgccctaa
    28921 tggtacggcg cataaatact ttgccagcgc accgtacaaa attgcggcga aatctggtac
    28981 cgctcaggtc ttcggtctga aagcgaacga aacctataat gcgcacaaaa ttgccgagcg
    29041 tttacgtgac cacaaactga tgaccgcctt tgcgccatac aacaatccgc aagtggctgt
    29101 cgccatgatt ctggagaacg gcggcgcggg tccggcggtt ggtacgctga tgcgccagat
    29161 cctcgaccac attatgctgg gtgataacaa caccgatctg cctgcggaaa atccagcggt
    29221 tgccgcagcg gaggaccatt aatcatgacg gataatccga ataaaaaaac attctgggat
    29281 aaagtccatc tcgatcccac aatgctgctg attttactgg cattgctggt ttacagcgcc
    29341 ctggttatct ggagcgccag cggtcaggat attggcatga tggagcgtaa aatcggccaa
    29401 atcgcgatgg gtctggtcat catggtggtg atggcgcaaa ttcctccacg cgtttatgaa
    29461 ggctgggccc cctatctcta tatcatctgt attattttgc tggtggcggt agatgctttc
    29521 ggagccatct ctaaaggcgc tcaacgctgg ctggacctcg gtattgttcg ttttcagccg
    29581 tcggaaattg ccaaaatagc cgtaccactg atggttgcgc gctttatcaa ccgcgacgtt
    29641 tgcccgccat cgttgaagaa cactggcatc gcgctggtgc tgatatttat gcccacgctg
    29701 ctggtggctg cacagcctga cctaggaaca tcaatcctcg ttgcgctttc cggtctgttt
    29761 gtactgttcc tctctggcct tagctggcgt ctgattggcg tcgcagtagt gctggtagcg
    29821 gcgttcattc cgattctgtg gttcttcctg atgcatgatt accagcgcca gcgcgtaatg
    29881 atgctcctgg acccggaatc agacccactc ggcgcgggct atcacattat tcagtctaaa
    29941 attgctattg gctccggcgg attacgcggc aaaggctggc tgcacggcac tcagtcacag
    30001 cttgaatttc tccccgaacg ccatactgac tttatcttcg cggtactggc ggaagagctg
    30061 ggattagtgg gcattctgat tctgctcgct ctctacattc tgctgatcat gcgcgggctg
    30121 tggatagccg ccagagcgca aaccaccttt ggtcgcgtca tggctggtgg cttaatgctg
    30181 atattattcg tttatgtctt cgtaaatatt ggtatggtaa gcggtattct gccggttgta
    30241 ggggttccgc tcccactggt cagttatgga ggatcggcgc taattgtgct gatggctggg
    30301 ttcgggattg taatgtcaat ccacacccac aggaaaatgt tgtcgaaaag cgtgtaagag
    30361 gtgcgcaatg cgtaagcagt ggctcgggat ctgcatcgcg gcaggaatgc tcgcggcatg
    30421 tacaagcgat gatggtcagc aacagacggt aagtgtaccg cagcctgcgg tatgtaacgg
    30481 ccccatagtt gaaattagcg gggcggaccc gcgtttcgaa ccactgaacg cgacggcaaa
    30541 tcaggattac cagcgcgacg gtaaaagcta caaaatcgtg caggatccgt ctcgatttag
    30601 ccaggcggga ctggcggcaa tctatgatgc cgaaccaggc agtaacctga cggcctctgg
    30661 cgaagctttc gatccgacaa agctgacggc ggcccatcca acgcttccga tccccagcta
    30721 cgccagaatc actaacctgg ctaacgggcg aatgatcgtg gtgcgcatta atgatcgcgg
    30781 cccttacggc aacgaccgcg ttatttcgct ttctcgcgcg gcagctgacc gtcttaacac
    30841 gtcaaacaac accaaagttc gtatcgatcc gattattgtc gcccaggatg gttcgctttc
    30901 tggtcctggt atggcttgta ccacagtcgc caaacagact tacgccctgc ctgcacctcc
    30961 cgatttaagc ggtggcgcgg gaacaagttc agtgtctggc ccgcagggtg acattcttcc
    31021 ggtcagtaat tcgacgctaa aaagcgaaga tccgaccggc gcgccggtaa ccagtagcgg
    31081 tttcctcggc gctccaacga ccttagcgcc tggtgtactg gaaggcagcg aaccgacgcc
    31141 tgcgccacag cccgttgtta cagctccgtc gacaacgcct gcaacctcgc ctgcaatggt
    31201 gacaccgcaa gccgcctcgc aaagcgccag cggcaacttt atggtgcaag tcggggccgt
    31261 aagcgatcag gctcgtgcgc aacagtacca acagcaactg ggacagaagt tcggcgtccc
    31321 cggtcgcgta actcaaaatg gcgcggtctg gcggatccag cttggcccat tcgccagcaa
    31381 agccgaagcc agtaccttgc agcaacgttt gcaaaccgaa gcccaattac agtcatttat
    31441 tactaccgcg cagtagcgta aagcaggcat ctgatgtgtc aatttctgta aatgacgtta
    31501 acaaagtcac gcggaaagtc agatgcctgc cggtagtgca tttgctatag tagggcactt
    31561 ttttaattcc atcacggatg tcgtagttca gaccatgaat accatttttt ccgctcgtat
    31621 catgaagcgc ctggcgctca ccacggctct ttgcacagcc tttatctctg ctgcacatgc
    31681 cgatgacctg aatatcaaaa ctatgatccc gggtgtaccg cagatcgatg cggagtccta
    31741 catcctgatt gactataact ccggcaaagt gctcgccgaa cagaacgcag atgtccgccg
    31801 cgatcctgcc agcctgacca aaatgatgac cagttacgtt atcggccagg caatgaaagc
    31861 cggtaaattt aaagaaactg atttagtcac tatcggcaac gacgcatggg ccaccggtaa
    31921 cccggtgttt aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg ttccggtttc
    31981 tcagctgatc cgcggtatta acctgcaatc gggtaacgat gcttgtgtcg ccatggccga
    32041 ttttgccgct ggtagccagg acgcttttgt tggcttgatg aacagctacg ttaacgcact
    32101 ggacctgaaa aatacccact tccagacggt acatggtctg gatgctgatg gtcagtacag
    32161 ctccgcgcga gatatggcgc tgatcggcca ggcattgatc cgtgacgtac cgaatgaata
    32221 ctcgatctat aaagaaaaag aatttacgtt taacggtatt cgccagctga accgtaacgg
    32281 cctgttatgg gataacagcc tgaatgtcga cggcatcaaa accggacaca ctgacaaagc
    32341 aggttacaac cttgttgctt ctgcgactga aggccagatg cgcttgattt ctgcggtgat
    32401 gggtggacgt acttttaaag gccgtgaagc cgaaagtaaa aaactgctaa cctggggctt
    32461 ccgtttcttt gaaaccgtta acccactgaa agtaggtaaa gagttcgcct ctgaaccggt
    32521 ttggtttggt gattctgatc gcgcttcgtt aggggttgat aaagacgtgt acctgaccat
    32581 tccgcgtggt cgcatgaaag atctgaaagc cagctatgtg ctgaacagca gtgaattgca
    32641 tgcgccgctg caaaagaatc aggtcgtcgg aactatcaac ttccagcttg atggcaaaac
    32701 gatcgagcaa cgcccgctgg ttgtgttgca agaaatcccg gaaggtaact tcttcggcaa
    32761 aatcattgat tacattaaat taatgttcca tcactggttt ggttaaaaat tgaacacttg
    32821 aaagtgtaat ttccgtcccc atatactaag catcagtaaa aaaactcccg ccttctggcg
    32881 ggagttgcta tttaattacg ttacgccgga gctgacatga aaaccaaact taacgaactg
    32941 cttgaattcc ctactccttt tacttacaaa gttatggggc aggcgttacc tgagctggtt
    33001 gatcaggtgg ttgaagtggt acagcgccat gcgccaggtg actacacccc aacggtaaaa
    33061 ccaagcagca aaggcaacta ccactcggta tctatcacta tcaacgccac tcatatcgag
    33121 caggttgaaa cactgtatga agaactgggc aaaatcgata ttgtccgcat ggttctgtaa
    33181 ctcgcttctc cgttacccgg ctcctggtcg ggtaactccc ctctcctcgc tgtgatatac
    33241 ttttccccca cttttactca ttctccacgg agatgccgtt ttgtatcagg ataaaattct
    33301 tgtccgccag ctcggtcttc agccttacga gccaatctcc cgggctatgc atgaattcac
    33361 cgatacccgc gatgatagta cccttgatga aatctggctg gtcgagcact atccggtatt
    33421 cacccaaggt caggcaggaa aagcggagca cattttaatg ccgggtgata ttccggtgat
    33481 ccagagcgat cgcggtgggc aggtgactta tcacgggccg ggacaacagg tgatgtatgt
    33541 gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa ctggtgacct tgcttgagca
    33601 aacagtggtg aataccctgg ctgaactggg tatagaagcg catcctcggg ctgacgcgcc
    33661 tggtgtctat gtcggggaaa agaaaatttg ctcactgggt ttacgaattc gacgcggttg
    33721 ttcattccac ggtctggcat taaacgtcaa tatggatctt tcaccatttt tacgtattaa
    33781 tccttgtggg tatgccggaa tggaaatggc taaaatatca caatggaaac ccgaagcgac
    33841 gactaataat attgctccac gtttactgga aaatatttta gcgctactaa acaatccgga
    33901 cttcgaatat attaccgctt aattccatac atcaatggcc taatttacac tgggtcatta
    33961 ctcataatac ctgctaatgc tttacatctc cttcgcgttt gtatattttc tttatcactt
    34021 gcagaaatgc ggtgccacgc cttgctccgt aactcacccc attgattaac ctattgaaat
    34081 agttgattat ggtgagcaaa agcgttccgt tccaattaga cataagatta ttgtaatttt
    34141 ttaaatatta tttttccatg aataaaattg gagtaagtgc gtggatagta ataatcaaat
    34201 tgaaccctgt ttaagcagaa agtcgtcaga aggtaaacca caaatattta caaccttacg
    34261 aaatattgat cttaaccttc tgactatttt tgaagctgta tatgtacata aagggatcgt
    34321 taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta ttcagaaact
    34381 gcgcgttata ttccctaacc cattgtttat tcgcaaaggc cagggtgtca ctcctaccgc
    34441 atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc ttggcgcgct
    34501 ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca ctccctcggt
    34561 cggagccctg gtccttcctg tcatctatcg ggcgattaaa actcactatc cgcagctttt
    34621 actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc aaaccgatct
    34681 catcatcgat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc tgttcaccga
    34741 caatatggtg ttaatttgcc gcgagggaaa tccactactc tctttagaag atgacagaga
    34801 gactatcgac aacgctgcgc atgtacttct gttaccggaa gggcaaaatt tcagcggtct
    34861 gcggcagaga gttcaagaga tgtttccgga ccggcaaatc aatttcacca gctacaacat
    34921 tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc ccagccgttt
    34981 ttataacctg tttagtcgtt gctggccgct ggaaaaactg ccttttccgt ccttaaatga
    35041 ggagcaaata gatttctcta tccactacaa caaattcagc ctgcgtgatc ccatcctgca
    35101 cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag ccagatcaca
    35161 gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg accgctttgg
    35221 ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa tagttgataa
    35281 ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg aacacgcacg
    35341 ctatgagtaa acccattgtg atggaacgcg gtgttaaata ccgcgatgcc gataagatgg
    35401 cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc aagccggaat
    35461 ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa gccgcaatgc
    35521 gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg gcggaatgct
    35581 tcaaccacgg cacagcaacg tttatgatcc tcggcgctat ttgtacccgc cgttgtccgt
    35641 tctgtgatgt tgcccacggt cgcccggtag ctcctgatgc caatgaacca gtgaaactgg
    35701 cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt gaccgtgatg
    35761 acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt cgggaaaaaa
    35821 gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgaatg gatcgtgctc
    35881 tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa aacgtaccgc
    35941 gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag ctgctggaac
    36001 gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg ggtctgggtg
    36061 aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt gtgacgatgt
    36121 taacgctggg gcaatatttg cagccaagcc gccatcacct gccggttcaa cgttacgtta
    36181 gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt acccatgctg
    36241 catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa gggatggaag
    36301 ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct ttttgaagcg
    36361 ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc actctttatg
    36421 agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag catcgtcatc
    36481 attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca gcgtacgtaa
    36541 cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt tggtaatact
    36601 aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc cttaaaaacc
    36661 agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac aaggtgaatc
    36721 aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg gttgtttaag
    36781 atgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc cataatgagt
    36841 gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat ccggctttgc
    36901 ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt atccagcgca
    36961 cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccacgaac ccaggcggca
    37021 ggaagtacca ggatttcagc tccctgtaaa gcctgtgcca gcgccagctc tggaaagcgt
    37081 aagtcataac aggtcatcag accgaccttc atcccttcca cctccagtaa cggagctatt
    37141 tcattaccag catcaacatg gcgtgattcc tgaatggcaa atgcatcata gagatgcagt
    37201 ttggcataac gggcgacgat gtttcctgcc tgaagtgcca ccagcatatt ccatgcgcgc
    37261 cccggcgttg aaggaagatg aatcgtcaga attgtcgtca tcatgttacg tttactttct
    37321 cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac cgatagatct
    37381 gcatcatgat catcgcgcgc cagcaatgct tccggcagga caaacagcga tacgtcgttt
    37441 tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca cacagatgta
    37501 acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg taaaattcgc
    37561 caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc aggattttgc
    37621 agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg tagcgagatg
    37681 gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga cgctggcagc
    37741 aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca ggatgacgaa
    37801 cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc taacaacctt
    37861 ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg gctgggcatt
    37921 actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg cattctggct
    37981 gttttcggcc tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa gcgggttttg
    38041 aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc ctttggcacc
    38101 gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac cattagtctg
    38161 gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa tgaaaccgaa
    38221 tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca aaattacctt
    38281 tacatgaaaa atagacacaa atgctgtgtc ggttatcagt acaccaattg tggtacgctt
    38341 tgtccagtgc aactttacta aaaagtgata aaagtcgcgt tatttttgat gttgcttatt
    38401 ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact gaaagcgcat
    38461 ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca tctgaaaagt
    38521 cactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga tgtaattcca
    38581 gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat tttcatccgc
    38641 cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt tgttccacga
    38701 gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc aacgcgacag
    38761 gccaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt tgtcgtaagc
    38821 gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag gctgttgcca
    38881 ggtttgtgca atattttctc taaacgttgt catcctctca tctgcgttag caaaaacttt
    38941 accaacgctg attaactgaa taaaaacaaa ggaaaagata gcgacatatt tactcacgtt
    39001 catttgtgac cataaaacat ttatcaaaaa tctactacta gcatagcaaa gctacaatta
    39061 acataacctt aatagaccca acataaagaa taatctgaat agctgctcta tttatttccg
    39121 cttgttcaag ttaaatattt ccgcgcatcg attaaagatg atgcgtcgtt ttctgcgatg
    39181 ggaatagtca aaaaagaaaa accaagtctt ttttgatgac aaaatgcaat caaggaaaaa
    39241 ttattttatt ttttaacgag atacaacaat catcttaacg aagtatataa tatatatcgt
    39301 caacccatgc tcatattaga ttattttttg agcgcacagc cgcagctaac accattgcaa
    39361 ttaacaaatt tgcatcaatc gccatcaatt tacacacatt attatgtgat aattgccaac
    39421 cgctaaatat gcgttttgtt atctatgtat aaaaacagca acttcaatgt cttaatggca
    39481 gtttttcttg attttaatca gcattcatcg ccaatttatt gggcatattt tttccttaag
    39541 ctttaggaat tttttattat ttactttggg cctggagaca ggaaaaatta tgctgacatt
    39601 cattgaactc cttattgggg ttgtggttat tgtgggtgta gctcgctaca tcattaaagg
    39661 gtattccgcc actggtgtgt tatttgtcgg tggcctgtta ttgctgatta tcagtgccat
    39721 tatggggcac aatgtgttac cgtccagcca ggcttcaaca ggctacagcg ccacggatat
    39781 cgttgaatac gttaaaatat tactaatgag ccgcggcggc gacctcggca tgatgattat
    39841 gatgctgtgt ggctttgccg cttacatgac ccatatcggc gcgaatgata tggtggtcaa
    39901 gctggcgtca aaaccattgc agtatattaa ctccccttac ctgctgatga ttgccgccta
    39961 ttttgttgct tgtctgatgt cactggccgt ctcttccgca accggtctgg gtgttttgct
    40021 gatggcaacc ctgtttccgg tgatggtaaa cgttggtatc agtcgtggcg cagcagctgc
    40081 catttgtgcc tccccggcgg cgattattct cgcaccgact tcaggggatg tggtgctggc
    40141 ggcgcaggct tccgaaatgt cgctgattga cttcgccttc aaaacgacgc tgcctatctc
    40201 aattgctgca attatcggca tggcgatcgc ccacttcttc tggcaacgtt atctggataa
    40261 aaaagagcac atctctcatg aaatgttaga tgtcagtgaa atcaccacca ctgcccccgc
    40321 gttttatgcc attttgccgt tcacgccgat catcggtgta ctgatttttg acggtaaatg
    40381 gggtccgcaa ttacacatca tcactattct ggtgatttgt atgctgattg cctccattct
    40441 ggagttcctc cgcagcttta atacccagaa agttttctct ggtctggaag tggcttatcg
    40501 cgggatggca gatgcgtttg ctaacgtggt gatgctgctg gttgccgctg gggtattcgc
    40561 tcaggggctt agcaccatcg gctttattca aagtctgatt tctatcgcta cctcgtttgg
    40621 ttcggcgagt atcatcctga tgctggtatt ggtgattctg accatgctgg cggcagtcac
    40681 gaccggttca ggcaatgcgc cgttttatgc gtttgttgag atgatcccga aactggcgca
    40741 ctcttccggc attaacccgg cgtatttgac tatcccgatg ctgcaggcgt caaacctggg
    40801 tcgtacccta tcacccgttt ctggcgtagt cgttgcggtt gccgggatgg cgaaaatctc
    40861 accatttgaa gtcgtaaaac gcacctcggt gccggtgctt gttgggctag tgattgttat
    40921 cgttgctaca gagctgatgg tgccaggaac ggcagcagcg gtcacaggca agtaaatagt
    40981 aatgccggag gcaagtttcc tccggcttta tcatgtcacc cactgtggta tatgcgttgt
    41041 ggtctgccaa ctttgccgtg aacaatttca gcaataatca gatggcggct ggcgcaatat
    41101 tcaagataac gcctggcagt ggtgcggctg atggtcagtg cctgcgccac cgtttctgcc
    41161 gtatgttgca cacaaggctc tttaaacagt tttcgcaccg cgtttagcgt caaggggtca
    41221 atgccggtcg gcagttcgtc tttgggttca ccgcgagcat aggcattaaa catctcatca
    41281 atttgcttct ggctggcgct atcaatactt tccagcatat gtttacgctg gcggaaacgg
    41341 gttagcgttt gccccagtgt attgattcac ttgaagtacg aaaaaaccgg gagaacattg
    41401 gattattcgg gatctgatgg gattagattt ggtggggctt gcaagcctgt agtacaaatt
    41461 ttaggcgtta accaatgaaa cgcgaaagat agtaaaaaat tgcttttgtt tcattgaaaa
    41521 aacgaaaaac aaaaacacta taaatc
//
LOCUS       DADLSB010000017        40654 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.017, whole genome shotgun sequence.
ACCESSION   DADLSB010000017 DADLSB010000000
VERSION     DADLSB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.017"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(163..954)
                     /gene="cpoB"
                     /locus_tag="JN331_000923"
     CDS             complement(163..954)
                     /gene="cpoB"
                     /locus_tag="JN331_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY7259130.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNATIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(964..1485)
                     /gene="pal"
                     /locus_tag="JN331_000924"
     CDS             complement(964..1485)
                     /gene="pal"
                     /locus_tag="JN331_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY7259131.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1520..2812)
                     /gene="tolB"
                     /locus_tag="JN331_000925"
     CDS             complement(1520..2812)
                     /gene="tolB"
                     /locus_tag="JN331_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY7259132.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGLGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2945..4186)
                     /gene="tolA"
                     /locus_tag="JN331_000926"
     CDS             complement(2945..4186)
                     /gene="tolA"
                     /locus_tag="JN331_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY7259133.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4251..4679)
                     /gene="tolR"
                     /locus_tag="JN331_000927"
     CDS             complement(4251..4679)
                     /gene="tolR"
                     /locus_tag="JN331_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY7259134.1"
                     /translation="MARARGRGCRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4683..5375)
                     /gene="tolQ"
                     /locus_tag="JN331_000928"
     CDS             complement(4683..5375)
                     /gene="tolQ"
                     /locus_tag="JN331_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY7259135.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5372..5776)
                     /gene="ybgC"
                     /locus_tag="JN331_000929"
     CDS             complement(5372..5776)
                     /gene="ybgC"
                     /locus_tag="JN331_000929"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY7259136.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5926..6219)
                     /gene="ybgE"
                     /locus_tag="JN331_000930"
     CDS             complement(5926..6219)
                     /gene="ybgE"
                     /locus_tag="JN331_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY7259137.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6219..6332)
                     /gene="cydX"
                     /locus_tag="JN331_000931"
     CDS             complement(6219..6332)
                     /gene="cydX"
                     /locus_tag="JN331_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY7259138.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6347..7486)
                     /gene="cydB"
                     /locus_tag="JN331_000932"
     CDS             complement(6347..7486)
                     /gene="cydB"
                     /locus_tag="JN331_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7259139.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7502..9070)
                     /gene="cydA"
                     /locus_tag="JN331_000933"
     CDS             complement(7502..9070)
                     /gene="cydA"
                     /locus_tag="JN331_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY7259140.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(9919..12552)
                     /gene="mngB"
                     /locus_tag="JN331_000934"
     CDS             complement(9919..12552)
                     /gene="mngB"
                     /locus_tag="JN331_000934"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAY7259141.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKSENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIDSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12570..14547)
                     /gene="mngA"
                     /locus_tag="JN331_000935"
                     /pseudo
     CDS             complement(12570..14547)
                     /gene="mngA"
                     /locus_tag="JN331_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
     gene            14657..15379
                     /gene="mngR"
                     /locus_tag="JN331_000936"
     CDS             14657..15379
                     /gene="mngR"
                     /locus_tag="JN331_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY7259142.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15483..16352)
                     /gene="sucD"
                     /locus_tag="JN331_000937"
     CDS             complement(15483..16352)
                     /gene="sucD"
                     /locus_tag="JN331_000937"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY7259143.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPEGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16352..17518)
                     /gene="sucC"
                     /locus_tag="JN331_000938"
     CDS             complement(16352..17518)
                     /gene="sucC"
                     /locus_tag="JN331_000938"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY7259144.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMCDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17612..18829)
                     /gene="odhB"
                     /locus_tag="JN331_000939"
     CDS             complement(17612..18829)
                     /gene="odhB"
                     /locus_tag="JN331_000939"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY7259145.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(18844..21645)
                     /gene="sucA"
                     /locus_tag="JN331_000940"
     CDS             complement(18844..21645)
                     /gene="sucA"
                     /locus_tag="JN331_000940"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY7259146.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVVTLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSCLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(21946..22662)
                     /locus_tag="JN331_000941"
     CDS             complement(21946..22662)
                     /locus_tag="JN331_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY7259147.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22678..24444)
                     /gene="sdhA"
                     /locus_tag="JN331_000942"
     CDS             complement(22678..24444)
                     /gene="sdhA"
                     /locus_tag="JN331_000942"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY7259148.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24444..24791)
                     /gene="sdhD"
                     /locus_tag="JN331_000943"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24444..24791)
                     /gene="sdhD"
                     /locus_tag="JN331_000943"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY7259149.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24785..25189)
                     /gene="sdhC"
                     /locus_tag="JN331_000944"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24785..25189)
                     /gene="sdhC"
                     /locus_tag="JN331_000944"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7259150.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25570..25677
                     /locus_tag="JN331_000945"
     CDS             25570..25677
                     /locus_tag="JN331_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259151.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            25883..27166
                     /gene="gltA"
                     /locus_tag="JN331_000946"
     CDS             25883..27166
                     /gene="gltA"
                     /locus_tag="JN331_000946"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY7259152.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27556..28122
                     /locus_tag="JN331_000947"
     CDS             27556..28122
                     /locus_tag="JN331_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7259153.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28173..30524
                     /locus_tag="JN331_000948"
     CDS             28173..30524
                     /locus_tag="JN331_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7259154.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNFNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAEDVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEG
                     HAWLSGVAENQKVTVVWGDSQHCSLHLPEHMEDTANRLILPCH"
     gene            30539..31270
                     /locus_tag="JN331_000949"
     CDS             30539..31270
                     /locus_tag="JN331_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7259155.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVETKK"
     gene            31267..32328
                     /locus_tag="JN331_000950"
     CDS             31267..32328
                     /locus_tag="JN331_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7259156.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            32435..33526
                     /locus_tag="JN331_000951"
     CDS             32435..33526
                     /locus_tag="JN331_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY7259157.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(33523..34314)
                     /gene="nei"
                     /locus_tag="JN331_000952"
     CDS             complement(33523..34314)
                     /gene="nei"
                     /locus_tag="JN331_000952"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY7259158.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34350..35084)
                     /gene="pxpA"
                     /locus_tag="JN331_000953"
     CDS             complement(34350..35084)
                     /gene="pxpA"
                     /locus_tag="JN331_000953"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY7259159.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35074..36006)
                     /gene="pxpC"
                     /locus_tag="JN331_000954"
     CDS             complement(35074..36006)
                     /gene="pxpC"
                     /locus_tag="JN331_000954"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY7259160.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLFHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(36000..36656)
                     /gene="pxpB"
                     /locus_tag="JN331_000955"
     CDS             complement(36000..36656)
                     /gene="pxpB"
                     /locus_tag="JN331_000955"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY7259161.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDSARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(36679..37422)
                     /gene="ybgI"
                     /locus_tag="JN331_000956"
     CDS             complement(36679..37422)
                     /gene="ybgI"
                     /locus_tag="JN331_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY7259162.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            37693..39174
                     /gene="dtpD"
                     /locus_tag="JN331_000957"
     CDS             37693..39174
                     /gene="dtpD"
                     /locus_tag="JN331_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY7259163.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPDVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39324..>40654)
                     /gene="phrB"
                     /locus_tag="JN331_000958"
     CDS             complement(39324..>40654)
                     /gene="phrB"
                     /locus_tag="JN331_000958"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY7259164.1"
                     /translation="VLALYIATPRQWETHNMSPRQAELINAQLNGLQIALAEKGIPLL
                     FREVDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVDVERALRNVVCEGFDDS
                     VILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPKVRSSGSIKPAPSITLN
                     YPRQSFDTAHFPVEEKAAIAQLRQFCENGAGEYEQQRDFPAVEGTSRLSASLATGGLS
                     PRQCLHRLLAEQPQALDGGAGSVWLSELIWREFYRHLMTYYPSLCKHRPFIAWTDRVQ
                     WQSNPAHLQAWQKGKTGYPIIDAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGE
                     RYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPITQGEKFDREGEFIRRWLPE
                     LRDVPGKAVHEPWKWAQKAGVKLDYPQPIVEHKEARIQTLAAYEAARKGK"
BASE COUNT         9820 a        11003 c         9853 g         9978 t
ORIGIN      
        1 tttttcgttg ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt
       61 tcagacttga gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg
      121 acacgaccag aaataatgcg acttctggtc gtgtgttatg cattacatcg cgttcagacg
      181 tttttgtgcc tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac
      241 ggctttcgct tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc
      301 atcagcagcc tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc
      361 atcatctttt ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag
      421 gtaagttgaa tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg
      481 gcgggattta tcctgcacca gcgcaatagt tgcattgtaa tccgtgtttg catcgccgct
      541 tttcaccggc gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc
      601 cgcaccgctt tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc
      661 gatctgcaac aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc
      721 ctgaatctga ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag
      781 ttgggttaaa agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc
      841 gaccgagcct gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc
      901 tataccaacc agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt
      961 ctcttagtaa accagtaccg cacgacggtt tttggagtat gccgcttcgt catgacccag
     1021 tactgcaggt ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt
     1081 accctgcagg tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc
     1141 cggagtacca cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg
     1201 caggaagttt gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc
     1261 cagatcgaag taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc
     1321 ttcggaagac atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat
     1381 gccttcgctg ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac
     1441 aggcagagca atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt
     1501 actattcaat taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat
     1561 cagttgccgg aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc
     1621 ccatcccctg agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt
     1681 catctaggaa cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa
     1741 tgtgctgctg cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat
     1801 ccgcatcctg gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat
     1861 tcactttata aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctat
     1921 ccgggaacca ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac
     1981 cagaagccaa atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca
     2041 gtttgctgcc gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct
     2101 gacgtacagc gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg
     2161 tcacataagc cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg
     2221 aacggtgaac gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga
     2281 actgaccgcc gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc
     2341 cggtcagttt ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct
     2401 tgttcacttt gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa
     2461 gttgataagc aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag
     2521 cgtcaatgcc cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct
     2581 gtggcagacg agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa
     2641 cgatgccgcc aatatcttca ggtgccgcac caagccccgc ccactggaaa ggaacaacac
     2701 caataggacg accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa
     2761 ctgatgccca cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc
     2821 ccttatctgg accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca
     2881 aaactaccag aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc
     2941 gcgattacgg tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg
     3001 gtggtttcgg gatcttagca agtttagctg ctgccaacgc agcctgacaa agtgcgggat
     3061 cgccaccttc aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg
     3121 tacaggtttt gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa
     3181 tctgcccggc atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac
     3241 tcccggcagg cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac
     3301 cagagcttag ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt
     3361 tttcagctgc tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg
     3421 ctttcttatc agctgctgcc ttatcagcag ccgctttttc tgctgctttt ttgtcggctg
     3481 cagctttctc tgctgccgct ttcttatcag ctgcagcctt ttcagcagcc gctttcttct
     3541 cagcttctgc cttggctttt tcagcagctt cagttgccgc tttctttctt gcttcagctg
     3601 cagctgcttc tgccgcttcc gctttcttct tcagtgccgc agcggctacc tcggcttttt
     3661 tctgcgcttc ggctgcggct ttggcggctt ctgcttctgc ttttttcttc gcgtctgcag
     3721 ccgctttctt cgctgcttct tccgcagctt tagcatctgc cgccgctttc gctgccgcca
     3781 cttcagcttg cttctgcttt aactcggcct gttttgcggc ttcttcagcc tgctttttct
     3841 gctcctgagc cgctaaccgc tctttctcaa gttgcttcag gcgttcctgt tcagccgctt
     3901 gtttctcacg cagttcttca gcagcctgct gttccttcat cttgcgctgt tcatcagaac
     3961 gcttcgcgct tgattcctgg ctttgcatgc gtttgtactg ctcaactacc gcacctgaat
     4021 caaccatgac agcgtcgatg gacgaaccac cgccgcctcc ggctgaagct tctatattct
     4081 catcgaacga actccagatc agcgccgcaa ataagatgac atgcagcact gctgaaataa
     4141 ttatcgcccg cttgagcttg tcgttttgtt cggttgcctt tgacactctc ggtttccaaa
     4201 aactgttcgc ctgttacccg ctctctttca agcaagggaa acgcagatgt ttagataggc
     4261 tgcgtcatta aaccaaccga tttcacaccc gcactatgta acaagttcag tgctttaatt
     4321 atttcatcgt aaggcacatc ttttgcgcca ccgatcagaa agaccgtttt cgggttggcc
     4381 ttgaaacggc tggacacttc cgccaccacc tgctctggtg gtaaacgctc taggcgatct
     4441 ttctcaacca ccacggtgta ctgaccaata ccagacactt caacaatcac tggcggatta
     4501 tcgttactgc tcaccgcctg tgattcagta gcgtctggca gatcgacctc cacgctctgg
     4561 gtgatgatgg gcgctgtcgc cataaagatc agcaacagca ccagcagtac gtccagcaac
     4621 ggtacaatgt tgatttcgga cttgagatcg cgacaacctc gtccacgcgc tctggccatg
     4681 gcttacccct tgttgctctc gctaacggta aacgcctggc ggtgcagaat cgcggtaaac
     4741 tcttccataa agttgtcgta attcagttcc agtttgttta cgcgctggtt gagacggtta
     4801 taagccatta ccgccggaat tgcggcgaac agaccaattg ccgtcgcaat caacgcttct
     4861 gcgataccgg gcgcaaccat ttgcagtgtt gcttgtttta ccgccccgag ggcgataaag
     4921 gcgtgcatga tcccccagac cgtaccaaac agaccaatat acgggctgat ggagccaacc
     4981 gtaccgagga acggaatgtg cgtttccaga ttttcaagtt cacggttcat ggagatacgc
     5041 atagcacgcg acgccccttc cactacggct tccggcgcat ggctattggc acgatgcagg
     5101 cgcacaaact ctttgaaccc gctgtaaaag atttgttccg aaccagtcag attatcccgt
     5161 ttcccctgac tctcttgata gaggcgagag agttcgattc cagaccagaa tttatcttca
     5221 aacgcttcgg cttcacgcgc cgctgcgtta agaatacggg ttcgctggat aataatggcc
     5281 caggatgcga ttgaaaaacc aatcaaaatc aacatgataa gtttaaccag aaggctagcc
     5341 ttcaggaaca aatcaaggat attcatgtca gtcactgctt aaactccgcg acaatagact
     5401 tgggaagcgc acgaggcttc attttgagtg ggtcaacgca aacaaccaga acctctgctt
     5461 cattcagcaa agtattctcg gcgttgacaa tacgttgcgt gaaaaccaaa gaggtgccac
     5521 gcattgatgt tatttcagtc tgtatttcga gcatatcgtc gagccgcgca ggtgcgtaat
     5581 attccaccgt cattttacgt accacaaagg caacgcgttc agccatcagc gcctgctgac
     5641 tgaagtgatg atgacgcagc atctctgtgc gtgctctttc ataaaaagcg acgtaactgg
     5701 cgtggtacac cacaccaccg gcatcggtat cttcatagta gacgcgaacc ggccatcgaa
     5761 acagcgttgt attcacttta catcccggta atgcaacaaa agttagagct tttaaacgct
     5821 gctactatac gcgcgcgatt gaggtttggg aatgagggca agttaaggga gcgtaaaaat
     5881 ttatgggcct ggagcggccc ataatttttt aaagagaatt ctgatttaaa agaagaagaa
     5941 aatcagcccg acaatgagaa caatatcggc aagcaatggg caaaaaatcc cttgccaaag
     6001 aaccttctgc ggacgaaagc ccacgccgtg aatcacacca gcacagacgg cccacatcag
     6061 caataaacca tgccagattt ccagttcact ggtcttcgcg gcaaaacgag atgggtccca
     6121 aaacatacat cctgctaaca gaagcgccat cacgaaggaa agcgcccgca gggggcgctt
     6181 gtccattacc gcatacaagg tcgcgataat cttactcatc agatgtcttc ttgaccggct
     6241 ttgcctgatt cgacgtgttc aagcgccagc gcggtgatta ccccaaacga acaggcaaga
     6301 agcgttccca gaatccatgc gaaataccac atttttagct ccttacttag tacagagagt
     6361 gggtgttacg ttcaatatct tctttggtga tacgaccgaa cattttccag taacaccagg
     6421 cggtgtagag cagaatgatc ggtaccagaa ccaccgcaac ccaggtcatg acgttaagcg
     6481 tcagctggct ggaagttgca tcccacattg tcagacttgc gttcatcatg gtgctggacg
     6541 gcatcacaaa cgggaacatt gcgataccgg ctgtcaggat gatgcaggcc agcgtcaggg
     6601 aggagaacac aaacgcccac gcggctttat ccatacgtgc agtcaggatg gtcagcagcg
     6661 gcagaaccac acccagtgcc ggaatagccc acagaattgg cgtgttgttg aagttaacca
     6721 gccatgcgcc agcttcacga accacttctt tattcagtgg gttagaggct gcgtaatggt
     6781 ccattgtcga tttcacgaca taaccatcga taccgtacat cacccatacg ccagccagtg
     6841 cgaaacagac cagtgtcacc agcgcagcca cctgagccgt tgcacgggta cgcaggtgca
     6901 gttcgcccac ggtacgcatt tgcagatagg ttgcgccctg agtaatgatc atccctacgc
     6961 tcaccacgcc tgccagcagg ccgaacgggt taagcaactg gaagaagtta ccggtgtagt
     7021 acagacgcag atattcatca acgttgaacg gtacgccctg caacaggtta ccgaacgcta
     7081 caccaattac cagcggcgga acgaagctac caatgaagat gccccagtcc cacatgttac
     7141 gccagcgggt ttcttcaatc ttggagcggt agtcaaaacc gaccggacgg aagaacaaag
     7201 acgccagcac gaggatcatc gccacataga agccggagaa cgcagcggca tagaccatcg
     7261 gccaggcagc aaagagtgcg ccgcccgcgg tgatcagcca aacctggtta ccgtcccagt
     7321 gtggtgcaat ggagttaatc ataattcgac gctcggtgtc gttacgaccg aggaaacggg
     7381 tgagcatgcc cacccccatg tcgaaaccgt cagtgactgc aaaaccaatc agcagaacgc
     7441 caaccagcag ccaccagata aaacgcaata cttcataatc gatcatttga cgactcctgt
     7501 cttagcgtgc cggctgagta gtcgtggaag actgctcaaa gtgatagcga ccggttttca
     7561 ggctgcttgg gccgaggcgt gcaaacttga acattaagaa caattctgcc accaggaaca
     7621 gggtatacag gccgcaaatc agcaccattg agaagatgag atcgcctgcg gtcagtgacg
     7681 aattcgccac agctgtcggc agcacttcac cgatagccca cggttggcgg ccatattcag
     7741 ccacgaacca gcccgcttct acagcaatcc acggcagcgg aataccgtac agcgcggcgc
     7801 gcagaagcca ttttttctcg ccaatgcggt tgcggatgac actccagaaa gagagcgcga
     7861 tgattgccag cagcaggaag ccacacgcca ccatgatacg gaacgcaaag tacagcggcg
     7921 ctacacgcgg gatggagtct ttggttgcct gttgaatctg cgcttcagtc gcatcagcca
     7981 cgtttggcgt atagcgtttc agcagcagac cgtaaccgag gtctttcttc atgctattga
     8041 actggtcacg aaccgcctgg tcggtagaac cagaacgcag ttgttcgagc agagagtacg
     8101 ccttcatccc gttacgaatg cgttcttcat gctgcaccat cagctctttc aggccgataa
     8161 ccggggtatc cacggaacgc gttgcaatga tgcccagtgc gtaagggatc tgaatcgcaa
     8221 atttgttcgt ctcctcttcc tgatcaggaa tgccgaacag agtaaaggca gcaggcgcag
     8281 gttgcgtttc ccactcggct tcaatagcag ccagtttggt tttctgcacg tcgcccattt
     8341 cgtagccgga ttcatcaccc agaacaataa cagacagaac agcagccata ccgaagctgg
     8401 cagcgatagc aaaggagcgt ttagcgaagg cgaagtcacg acctttcagc atataccatg
     8461 cgctgatacc gaggatgaac atcgcgccag tcacataacc agacgctaca gtgtgaacga
     8521 atttcacctg agcaaccggg ttaagcacca gctcggagaa gctcaccatc tccatacgca
     8581 tagtttcaaa gttgaaatcg gacgcgattg ggttttgcat ccagccgttc gcaaccagaa
     8641 tccacagtgc ggacaggttt gaaccgagcg ccaccagcca ggtgacacac atatgctgaa
     8701 ctttacccag acgatcccaa ccgaagaaga acagacctac aaaggtggat tcgaggaaga
     8761 aggccatcag accttcgatt gccagcggcg caccgaagat atcccctaca tagtgggaat
     8821 agtaagacca gttagtcccg aactggaact ccatggtcag accggtagcc acacccagag
     8881 cgaagttgat accaaacaac ttgccccaga acttggtcat atctttataa atctgtttgc
     8941 cggagaggac gtagaccgtt tccataatgg ccaggaggaa cgccataccg agcgtcagtg
     9001 gcacaaaaag gaagtggtac atcgcggtca aggcaaactg taagcgcgac agttcgacta
     9061 tatctaacat catgactcct tgctcatcgc atgaagactc cgagagtgaa ccccgttaga
     9121 aagggtcaca cgcatgcccc aatagaaatt tgtttgaaca ctttcatcat accatgtgaa
     9181 tgcacacaaa aacggcgtaa agaaaggttg caaacatgtt aataaaaact caaattgatc
     9241 ccacgtatat attacgccgc aaaatcctta caataaacag gtttttgttg aagcaaattt
     9301 gcatttttcg acggtgatca atttatagct aaattgccgc ctttcagcca atttgatcga
     9361 gaacaattta tctctttttg atgcccattt ccaagactta tacattgata aatatcaatt
     9421 tctacaaatt atgttaatca tgtttcaata aggtaacttt agtgacattt atgtttaaaa
     9481 tgtgtgaatt ataaggctga aattagtttc ggttggcaat taaagataaa aagagacgaa
     9541 gcaaaaacag cacggttgac cttaattctt tttttggtca ctggctaata cgacgcagag
     9601 tttcaggatt attaataaag gcataaatct cccccccgcc gatattaaca tttaattaac
     9661 ttataattat attataatta actcacccat ggttattatt ttgttaaaaa taaagccaca
     9721 tatacgtggc ttgagagtga agataaacgc aaaattacct gatgcgctac gcttgccagt
     9781 gtctacatca tctctgcaat atattgaatt tgcctgcttt tgtagaccga ataaagcgtt
     9841 catgccgtat ccggcatgaa caaagcgccc tttgtcaaca atctggccgc gcatatgtgg
     9901 cttcggtttg attgctattc aggcaagccg gtaactgaac gtccgtgact ggccgggtaa
     9961 aaaaggcccc gatagatttg aaccttgatt ttcctcggtg gtaatgtgtt catccatcat
    10021 cgtttctgag caagaaatca cctcgcgact gaaagcaaca gtcgcatcac aggttgctga
    10081 ttcagccgga ttaaacagcc gtaaaatcac ttcttgtcgg tcttcagctt tcttaagtgc
    10141 gcttatcagg catcccactg gtggcatttt caacaaacta taactttccg gcacgttgaa
    10201 tccggctttg ttgagcttca tcgcatccca tgggattttg ttgtaacact gtactggagt
    10261 cagccatgct cgcgcctgct gagctacacc agcggcggtt ggcgtaccgg tataactcaa
    10321 taaacttagg cgacaagaaa gcagaccacg tagttgtgag tccgggactg gcattttaat
    10381 tcccgaaggc cgcctaggcc ttaaaagcag atcttctttc cccagtaagc ccacgccgcg
    10441 cagcaacgta atggcaaagg ttttcttctc ttcaccgatg acttcaaatt cacgtagccc
    10501 ttcgctaaag acagccatgc cgttacgccc ttcctgtaag gctgcatagt tgagcatatt
    10561 ccataccgga accggcgctt ctttccagcc ttcttgctgc cagttgttca ttgcactgtc
    10621 gttcacgggg cgcgttagcg aaccaaactg ggtatctgcc agaacactat cggtgttaaa
    10681 agatgtaggg atcaggacgc gaaggcgatg atcgtcagcc tggttatcaa ggttgatatc
    10741 cacatcaatg cgcctgttgt tatgactaag agtgacaacc aactcgacac ctactctgcc
    10801 agtggattgt cttgcgctgc gttgttgcag attacgcggc accgccattt catagcggat
    10861 aacagccctg ctctgccagg cctcatgaat aatctcgcat tgcggtttcg cattcgccga
    10921 agtaattacc cactcttctt ttgcgggtga atagtcatat tcatcaccat catcagagct
    10981 ttcttcaatt tgcaacaccc ggtcatagcg cacaccgcta tctttatcta ccagttgtag
    11041 cgttccatcc tcattgagcg caatttgcca gaaagcattt tccagtatcc cttcagcgtc
    11101 actttttgcc gcaatcacgt tgccaggctg attcgcttcg atataaagcg tgcgatagcc
    11161 catagaaggg acaatctggt tgatctgtat atcaaactcc ataaagggat cgtaattacc
    11221 gtaatgaact atttgccgat cgattaggcc tggatcgatc tcacgggcat ggcgaataaa
    11281 atacggtaca ggctgaccgc gatcgtctcg tagattaaac tggctggcgc gcagccgcac
    11341 agtggtgttg atcacttctt cccgcggcca gggcatcagg ttaaacagga cgagtttgtc
    11401 ggcgtcgctc tgcggcatgt tgtcggcgat tttacgcata tagaaacgca gcagattatc
    11461 cgccatgtct tcagccagtt cgaagcgggc gacgatttcg cgatgaactt tgtcactaca
    11521 gcagcagccg atgctgtcgt gggcatgatt ttttaagatc tctttccaca ttttctccag
    11581 caagccgtgg tggtattcaa aacccaacgt ccaggccagc gttgccagcg gttccagcag
    11641 attaacaatc ttattttcaa tacgtgcgtg ggcaactttg atatccatgc gcgtggaatc
    11701 gatggtgcga tgcacgcgca tatatttgcc atcaataaat tcccctttca gggttgccag
    11761 attatctcgc tgcgcttcga tcttctcaaa tacctcttca aagcggctca tcacaaactt
    11821 tcgttgagga taaatttcgc gcagtttctc catcacttcg aagatatttt gctgcaatgg
    11881 catctgatca tgcccattcg gcagcaaaat ctctttggtt acagacgctt tttccagcac
    11941 gtcaaaataa ctgtcgaggc gtttgcgtaa tccgttttcg tccgcaggta agtacttacc
    12001 gatggcataa cccaacggca tcacctgcgc cgtcacttcg ctaccgtcac tgctttgcca
    12061 caaaaactcg gttttatccg taccgtggcg ctcagaacat ccacgccaga acatggtgca
    12121 ggtaatgcca aatccattgt agatatgcgg cagttgcccg gacatgccaa aggaatcggg
    12181 taaataacct attttcatcg gctcgccaaa cgcgaggcag tcacgcattc cgtacatcag
    12241 attacggacg atggattccg cagaaacaat cgtggtatcg gtctgggtat accaggggcc
    12301 gataatcaac ttgccggctt ctaccagttt cttcacgcgg tctttgtttt ccgatttcac
    12361 cgcgaaataa tcttcgagga tcgccgtttg cccgtcgagt acgtaatatt tgtattcgtt
    12421 gtcctgttcc agtcggcaca ggatctcttc catattattg accagcagaa tgcgtgactc
    12481 ttcggtggtg aaataccact cccgatccca gtgcatatgc ggggtgatgt gaacgcgaga
    12541 taccgctttc atcgtcgttt cctttttgtt tatggcatta cgccatcagt cagatagttg
    12601 ccatgcttaa ccgcgtgacg ccgccagatc aggagaattg cagtcgagat tgcagccccc
    12661 accaatgccg caccaaacca gccaattgcc gccataacac cgcccgcgcc attatcatga
    12721 agtaaaaaga gcgagaaaat gccagcaccg ggtgtcgaaa ggccgatatt catcgcaccg
    12781 acaatagcgc ccgttaccat agagcccagc acaaacgaac caataacccg cagcggatct
    12841 tcaatcgcca tcgggatcgc cccttcggta atacctgcca gccctaacag ccaggtggat
    12901 ttcccggtct caatttcaaa ctctttaaac aggcgcggtg cgagcatcgt ggaagcggtt
    12961 acggtaaatg ccgaaaccat tttgacggag gcgaaaatgg catacgggcc gtaaacgccg
    13021 ttcgccattg cgcccaagca gaatgcataa gcggctttat tcactggccc tccaaggtca
    13081 aaggaacaca taaaaccgag aatggcaccc agcaacagcg cgttacttcc tgacagaccg
    13141 ttcagctagg cggtaagcga gttattgatc caggcgacag gttcccccac cacaaacagc
    13201 atcagactgc ccgctcccaa tgtaccgagc accgggtaga gataaaaagt caggaagcca
    13261 ttgaatttac tgctaagacg caagtgattt ttcatccagc gtatcaagta accggctatc
    13321 aatccgccaa cgaccgcgcc gagaaacccg gagccgatca tgttggcggc aagtccagcc
    13381 gcaaagcctg gcgctaacgc ccggtttatc tgccagcgaa taggcggtat aggctgccag
    13441 caccggtacc atcaaaattc cgagcagccc gccgcccagt ttgcggtaca tccacagcca
    13501 ggagttttct tcattaaaca gatcttgtag cccgaagatt tgcgacagta ataccgcgac
    13561 cgccagcacc gtcccccccg cgacaatcaa cggtacggca aaagagatcc cgctcaacag
    13621 tgcctgtttc agctccgttt tgacgctttt caccggttgc gtatcttgct gtacggtacg
    13681 cgtctcatcg ccacgctcga gggtaagcga ttgttggatc aacgcttctg catggcgaat
    13741 cggctcggca acagacacag aaagcgcagg aatgccgtta aaacgctcac tctccttgat
    13801 ggcgacttca gccgcaaaaa tacaggcagt tgcactattg agttgatccg ccgttaaacg
    13861 cccttcaata ccgttagcac cttgcttttc aacgtagaca ttcacgccga gtttgcgtcc
    13921 ggctttttcc agatactccg cagccatgta ggtgtgagca atacccgccg ggcaggccgt
    13981 tacgcaaacg atagttggcg cgttggaaaa agaggcagaa ggttgcgtgc ctcccttgtc
    14041 atccagcgcc gagagcaatt catcaggcgt cgtcgccgac tgtatacgcg cccgaatctc
    14101 atcatccgcc aggcgcgtgg tcagcgctgt cagcagttgc atgtgcgtag tacccgcttc
    14161 attgggggga atcgccagca acaccactaa atcaaccgct tccgggccat ccacgccttc
    14221 ccactgaagc ggctcgctga gtgtggcgac cgcaaacgcc gcttctttta ccgcagcagt
    14281 tttgccatgc ggcacagcca acccttcacc taaggcggtc gggccaaggc tttcacggcg
    14341 atacacttct ttcagaaatt gctcagtact ggaaattttc cccagagcag caagacgttg
    14401 agtcaacgcg tgaatggcct cttcacggct ggtaaagcgc gcattcagac acaacgcatc
    14461 gcggtgggtc agagtcgtca gattcatcat gatcctcact tgatcgtttt tatagtcgcg
    14521 ccagtgtgcc cgataaaaca acaccattgc gtgatcaatt tcacttgatt gaaattattt
    14581 acattgtatt tgtattattt acaggatgaa tttatcgggc ataatggcta aattcgtatt
    14641 aatgagatgc taaataatgg gacacaaacc cttataccga cagattgctg atcgcattcg
    14701 tgagcaaatt gcgcgtggcg agcttaaacc tggcgatgcg ttacctaccg aatcggcatt
    14761 acaaacagag tttggcgtca gccgggttac ggtgcgtcag gctttgcgcc agttagtcga
    14821 gcaacagatc ctcgaaagca ttcagggcag cgggacttac gtcaaagaag agcgggtcaa
    14881 ttacgatatt tttcagttaa ccagttttga tgaaaaactg tcggaccgtc acgtcgatac
    14941 acacagtgaa gttctgatat ttgaagtgat tccggctgac gattttcttc agcaacagct
    15001 acaaatcacc gtgcaggatc gtgtgtggca tgtgaagcgc gtgcgctatc gcaagcaaaa
    15061 gccaatggcg ctggaagaaa cctggatgcc gcttgcgttg ttcccggatc tcacctggca
    15121 ggtcatggaa aattcgaaat atcactttat tgaagaagtg aagaagatgg ttatcgatcg
    15181 tagcgaacag gaaatcattc ctctgatgcc aaccgaagag atgagccgtc tgcttaatat
    15241 cagccagaca aagccgattc ttgaaaaagt atcgcgtgga tatttagttg atggtcgggt
    15301 gtttgaatat agccgcaacg cttttaatac cgatgactat aaatttaccc tgatagcgca
    15361 gcgaaaatca tcgcgataag cacaaaaaag gccatcatac gatggccaac catgtcgaaa
    15421 ccgacagccc ttcgccattc ctgtagaggg aagcggcgag ggctatttct tattacagat
    15481 atttatttca gaacagtttt cagtgcttca ccgatatccg ccaggctgcg aacggttttc
    15541 acgcctgcgg cttccagagc agcgaatttc tcatccgcag tccctttccc accggcaatg
    15601 atcgcacccg cgtggcccat acgtttgcct tccggcgcag tcacaccagc gatgtaaccc
    15661 acaactggct tggtaacatg ctctttgatg tacgctgccg cttcttcttc agcgctaccg
    15721 ccgatctcac cgatcatcac gatcgcttcg gtctgcggat ctttttcgaa catttcgaga
    15781 atgtcgataa agttagagcc agggatcggg tcaccgccga taccgacaca ggtcgactga
    15841 ccgaaaccgt aatccgtggt ctgtttaacc gcttcatagg tcagtgtacc ggaacgggaa
    15901 acgataccca ctttacccgg tttgtgaatg tgacccggct ggataccgat tttgcattca
    15961 cccggggtga taacgcctgg gcagttcggg ccgatcatac gaacgcctgc ttcgtccagt
    16021 ttcactttca cggtcagcat atccagcgtc gggatgcctt cagtgatggt gataatcagt
    16081 ttgatgcctg catcgatagc ttccagaatg gagtctttgc agaacggtgc tggtacgtaa
    16141 ataacagacg cggaagcgcc agtagcagca acagcttcac gcacggtgtt gaacaccggc
    16201 aggccgaggt gggtagtgcc gcctttacct ggagttacgc caccaaccat tttagtgccg
    16261 tatgcaatgg cctgttctga gtggaaagtc ccctggctac cggtaaagcc ctggcagata
    16321 accttggtgt ttttatcaat taaaatggac attatttccc ctccactgcg gcaacaacct
    16381 gctgagctgc atccgtcaga ccttttgctg caataatatt caggccgctg tcagccagtt
    16441 tcttcgcgcc gagttcggcg ttgttacctt ccagacgtac cacgaccggt acgttaacac
    16501 ccacttccgc taccgcgccg atgataccgt cagcgatcag gtcgcaacga acgataccgc
    16561 cgaagatgtt aaccagaacg gctttcactt tgtcgtcaga gaggatgatt ttgaacgctt
    16621 cggttacacg ttctttggtt gcgccgccgc caacgtccag gaagttagcc ggttcgccgc
    16681 cgtgcagttt aactatgtcc atcgtaccca tcgccagacc tgcgccgtta accatacaac
    16741 cgatgttacc gtccagcgca acgtagttca gttcccactg tgcagcctgt gcttcacgcg
    16801 gatcttcctg cgactggtca cacatttcgc gcagatcagg ctggcggaac agggcgttgc
    16861 catcagcgcc cagtttgccg tcgaggcaaa tcagatcgcc ctgtttggtg atgaccagcg
    16921 ggttgatttc gatcagcgcc aggtcgcgct ccaggaaaat ggtcgccagg cccatgaaga
    16981 ttttggtgaa ctgctgaacc agtttacctt ccagacccag tttgaacgcc agctcgcgtc
    17041 cctgatacgg catcgggcca gtcagcggat caagcgcaac tttatggatc aggtgcggag
    17101 tttcttccgc cactttttcg atttccacgc cgccttcggt ggaggccata aagaccacgc
    17161 gacgggaact acggtctaca acggcaccga gatacagctc tttagcgata tcggtcgccg
    17221 cttcaaccag aatctggtta accggttggc cattggcatc tgtttgatac gttaccagac
    17281 gcttgcccag ccagttttct gcaaaagcac ggatgtcttc tttgctgttt acaactttca
    17341 caccgcccgc tttaccgcgg ccaccagcgt gaacctgaca tttcactacc cacggaccgg
    17401 caccgatttt tgaagcggct tcttctgctt cgcgcggagt agtacaggca taacccaccg
    17461 gtgctggtaa gccatagcgg gcaaacagtt gttttgcctg atattcatgt aagttcatgt
    17521 gttctgtcca tccttcagta atcgttatct tttaaaccgt agaccgacac cctatccacg
    17581 tcggtctaca gtgcaggtga agcttaaact actacacgtc cagcagcaga cgcgtcggat
    17641 cttccagcaa ctctttgatc gttaccagga agcccacgga ttcgcgacca tcgatcagac
    17701 ggtgatcgta ggacagcgcc aggtacatca tcggcaggat ctcaacctga ccattcaccg
    17761 ccatcggacg atctttgata gcgtgcatac ccagaattgc gctctgcggc gggttgatga
    17821 tcggcgtaga catcagggaa ccgaacacac caccgttggt gatggtgaag ttaccaccgg
    17881 tcagatcttc aaccgtcagc ttgccatcac ggcctttgac tgccagctct ttgattttct
    17941 tctcgatgtc tgccatgccg agggtatcga catcacgcag aaccggcgtc accaggccgc
    18001 gcggcgtaga aaccgccatg ctgacgtcga aatagttgtg gtaaaccacg tcatcgccgt
    18061 cgatagacgc gttcacttcc gggtaacgtt tcagggcttc aaccaccgct ttcacgtaga
    18121 aggacataaa gcccagacgg atgccgtggc gtttttcaaa cgcttcaccg tactgcttac
    18181 gcagatccat aatcggcttc atgttgactt cgttgaacgt ggtcagcatg gcggtggagt
    18241 ttttcgcttc cagcagacgc tctgccacac gcttacgcag gcgagtcatc gggacacgtt
    18301 tttcactacg tgcagccaga gccggttgcg ccgccggagc agccgctgcc ggagcagact
    18361 ctttcgccgg ggctttcgcc agatgttttt ccacatcttc acgagtcaga cgaccaccca
    18421 caccggtgcc tttaatggcg ctggcgtcga gattgtgttc agccagcagg cgacggatcg
    18481 ccgggcttaa cgcatcgttg ttttgctctt ccagagacgc ctgctggcgt tgcgccggag
    18541 tggacgcttt ctcttcagat ttggcgctgg tttctttacc ggcgctgttg ccttcacgca
    18601 ggcgaccaag gatctgacga gacgttaccg ttgtaccttc atcttccaga accgcatcca
    18661 gaatgccgtc tgctgatgcc ggtacttcca gtaccacttt gtcagtttcg atttctacca
    18721 gcacttcatc acgtacgact gcgtcgccgg gttttttatg ccaggttgcg acggtggcat
    18781 cggctacgga ttcaggcagg tcagggacca gaatatctac gctactcatt gtgtatcctt
    18841 tatttattcg acgttcagcg cgtcattaac cagatcttgt tgctgtttct ggtgaacgga
    18901 catataccct accgccggag aggcggaggc cgggcggcct gcataacgca gagaagcccc
    18961 aaacggaatc acttcacgga aatgatgctg gctgcagtac catgcgccct ggttgagcgg
    19021 ctcttcctgg caccagacaa aatccttgac gtgagcaaac tgctgcaaca cttcctgcat
    19081 cgctttatgc gggaacgggt agagttgctc gatacgcaca atggcgacat cgtgttgatt
    19141 gttcttacga cgctgttcca gcaggtcgta ataaacctta ccagaacaca tcactacgcg
    19201 cttcacgccc ttcggatcaa gctcgtcgat ttcaccgatg gctggcagga aggtgccgtt
    19261 cgccagttct tcgaggcagg aaaccgccag cggatgacgc agcagggatt tcggcgacat
    19321 cacgaccagc ggacgacgca tcccgcgcag cgcctgacga cgcagcatgt ggtaaacctg
    19381 tgccggggta gacggtacgc aaacctgcat gttttgctca gcacaaagtt gcagataacg
    19441 ttccagacgc gcggaggagt gctccggccc ctgcccttcg taaccgtgcg gcagcaacat
    19501 caccagacca cacatccggc cccatttctg ctcgccagag gagatgaact ggtcgataac
    19561 cacctgcgca ccgttggcga agtcaccgaa ctgcgcttcc cagatggtca gggtgcgtgg
    19621 ttctgcggtg gcataaccat attcaaacgc cagcacggct tcttctgaca gtacggagtc
    19681 ccagacacgg aacgcgccct gaccgttatg gatgtgttgc agcggcgtgt aagtggaacc
    19741 gttagactgg ttgtggatca ccgcgtggcg gtggaagaag gtaccgcgac cggagtcttc
    19801 acccgacagg cgaaccggaa tgccttcatc aaccagcgtg gcgtaagcga ggttttccgc
    19861 accgccccag tcgaacagtt tctcaccggc tgccatcgcc tggcgatcgc cataaatctt
    19921 ggcaacgcga gactgcattt caactgcttc tggtaccgta ctgatacgtt tagccagttc
    19981 ctgcaggcgc ttcatctcaa ctttgttcgg gtactcttcg tcccattcgt ggttgaggta
    20041 cggcgaccag gtgaaagagt gcatgttcat cggacgccac tctgccacta cgcaatcgcc
    20101 agcatccagc gcatcgcggt acaggttaac catctcggtg gcatcttcca gcgtcaccac
    20161 tttttcctgc tccagcttgt cagcgtagat tttgcgcggt gtcggatgtt ttttgatttt
    20221 ctgatacatc agtggctggg ttgcgctcgg ctcgtcggct tcgttgtggc cgtgacggcg
    20281 gtagcatacc aggtcgatga agacatcacg tttaaaggtg ttacggaaat cgagcgccag
    20341 acgggtcaca aaggcaacgg cttccggatc atccgcgtta acgtggaaaa tcggtgcctg
    20401 aaccatctta ccgatatcag tacagtacgg tgtagaacgg gcatccagcg ggttagaggt
    20461 ggtgaaacca acctggttgt tgataacgat acgtaccgta ccgccaactt cataaccacg
    20521 cgctttcgac atgttcagag tttcctgaac cacgccctgc ccggtcactg cggcgtcacc
    20581 gtggatggtg attggcagca ctttgttgct gctcggctca tcaagtctgt ccagacgagc
    20641 acgaacagag ccgataacga ccgggcttac aatctcaagg tgagacgggt taaacgccag
    20701 cgccaggtgc accaggccgc catcggtctg gaagtcagac gagaagccca tgtggtattt
    20761 cacgtcgcct gtgccgaggt gttctttatg tttaccggcg aactcgtcga acaagtcttg
    20821 cggtttttta cccagcacgt tcaccagcac gttcagacga ccacggtgcg ccatcccgag
    20881 aaccacttcg cgggtgccgc tgttgccagc gtggcggatc atctctttaa gcatcgggat
    20941 taacgcgtca ccgccttcca gcgagaagcg ttttgcgcca gggaattttg cgccgaggta
    21001 acgctcaagg ccttcagcgg cggtcagttc gcttaagaag cgttttttct cttcgctatt
    21061 gaaagtcgcg cgaccagact cgatacgctg ttggatccag cgtttttctt cggtgctggt
    21121 gatgtgcata tactcggcac caatcgggcc gcagtaggtt tgcttgaggg cggaaataag
    21181 ctcgccgagt ttcatggttt ctttgccgct ggcaaatgaa ccgacgttga aggtttcctg
    21241 gaagtctgct tcggtcagat cgtggaaaga cggatccaga tcggccactt tatcttgctg
    21301 ccacagtccc agcggatcta gattcgcatg ctggtgacca cggaagcggt atgcgttaat
    21361 gagctgcagg actttaacct gcttcacatt ggtatcaggg tcggagatcg ttgaagagta
    21421 acgtgaagcg tctttcgcca ggcggcggaa atattcacgc gtttgagagt ggaattgatc
    21481 cggtttgact cccgtaccag gtaactgctg gaacgtcgaa cgccagttag cgtcaaccga
    21541 gtcaggatcg gttaagaagt cttcatagag ctgttctatc cagctctggt ttgcgccaga
    21601 gaggtaagaa gagtccaacc aggctttcaa agcgctgttc tgcatcgtga tcccttaagc
    21661 atctttttta tgcttacttc gccgtggata ctaccacgca cagtgatgtg cgtgccgggg
    21721 ttcacttgcg agctcttgtc tacgtagtgg ctcgcgaagg aacctttaaa aactgtctta
    21781 gtgtcagggg cagtttttaa agatttcctg catttctttg ttacgcctga tgcgctgcgc
    21841 ttatcaggcc tgcgggtaat gacttgtagg ccggataagg cgttcacgcc gcatccggca
    21901 ctggttgcct gatgcaacgc ttgcgcgtct tatcaggcct acagtttacg cattacgttg
    21961 caacaacatc gacttgatat ggccgatggc gcgcgtcggg ttcagcccct tcggacatac
    22021 actgacgcag ttcatgatgc tgtgacagcg gaatacgctg aatgcatcgc tcaaaccgtc
    22081 gaggcggctg tcagtctcgg tatcacggct atcgatcagg aaacgatatg ccgctaacaa
    22141 gcctgccggg ccgataaact tatcgggatt ccaccagaaa gacggacaag aggttgaaca
    22201 acatgcgcag agaatacatt catacagccc gtcgagtttt tcgcgctgct ctggcatctg
    22261 taaatgctcg cgagctggcg gattttgtcc attattcaac aggtaaggct taattttctc
    22321 atattgcgca tagaattgtc ccatgtctac caccaaatcg cggatcaccg gtaaacctgg
    22381 cagcgggcga atcacaatct tcttgcccgg ctggttgagt gccgaaatcg gggtaataca
    22441 ggccagacca ttcttgccgt tcatgttcag accgtcggaa ccgcacacac cttcacggca
    22501 ggagcggcgg aacgacaggc tgggatcttt ctctttcagc tggataagcg catccagcag
    22561 catcatgtca cgaccttctt ccgcttccag ggtgtaatcc tgcatacgcg gagcatcatc
    22621 aacatccggg ttatagcgat aaattgaaaa ctcgagtctc attttcctgt ctccgcatta
    22681 gtaagtacga atcttcggcg ggaatgccgg gcgcagtttc ggttccatgt tgacgcttcg
    22741 gcgcgtcatg gattccgact caggcagata cagggagtgg cacagccagt tttcatcatc
    22801 acgatccggg aagtcgaagc ggctatgtgc gccacggctt tcggtacgga agttggcaga
    22861 aacagccgtt gcatacgccg tttccatcag gttatccagt tccaggcact caacgcgctg
    22921 ggtgttgaac tcgctggaag tgtcatccag acgggcattt ttcagacgct cgcggatcac
    22981 tttcaactgc tcaagccctt tcgccatcgc atcaccttca cggaagaccg agaagttatg
    23041 ctgcatacat tcttgcagcg ctttacggat cgccaccgga tcttcaccgt tacgattatt
    23101 gttccagcgg ttcaggcgat ccagagacgc ttcaacatca gactcgctgg catcgcgcag
    23161 tgcgccctgc tcggcgatag actcttgcag atgcagacct gccgcgcgac caaagaccac
    23221 caggtccagc agcgagttgc cgcccagacg gttagcgccg tgtaccgata cacaagcgat
    23281 ttcaccaacg gcaaacagtc ccggaacaac cacatcttcg cctttctcat tcacagtcag
    23341 cgcctgaccg gtaactttgg tcggaatacc gcccatcatg tagtgacagg ttgggataac
    23401 cggaatcggc tctttcaccg gatcgacgtg agcgaaggta cgggaaagct ccaggatacc
    23461 cggcagacgg gattcgagaa cttctttacc caggtgatcg agtttcagtt tcgcgtgtgg
    23521 cccccacgga ccatcacagc cgcgaccttc acggatttca atcatgatgg aacgcgccac
    23581 cacatcacgg cccgccaggt ctttggcgtt cggcgcataa cgctccataa agcgttcgcc
    23641 atgtttgttc agcagataac cgccttcacc acggcaacct tcggtgacca gtacgcccgc
    23701 accggcaatg ccggtcgggt ggaattgcca catttccata tcctgcaccg gtacgcctgc
    23761 gcggatagcc atgccgacac cgtcgccagt gttaatgtgg gcgttagtgg tggactgata
    23821 aatacgccct gccccgccag tcgccagcac ggtagcgcgg gctttgaaat aaaccacttc
    23881 accggtttcg atgcacagtg cggtacaacc caccaccgcg ccatcctggt ttttcaccag
    23941 atccagcgca taccactcgg agaaaatggt ggtgtggttt ttcaggttct gctgataaag
    24001 cgtgtgcaac agtgcgtgac cggtacggtc agcagccgcc gcagtgcgtg ccgcctgctc
    24061 gccgccgaag tttttcgact gaccgccaaa cggacgttga tagatacgac catcatcgag
    24121 acgcgagaac ggcaggccca tgtgttcgag ttccagaatc gcttccggcc cggttttaca
    24181 catatattca atcgcgtcct ggtcaccgat atagtccgac ccttttacgg tgtcgtacat
    24241 atgccattcc cagttatctt catgggtatt acccagcgca acggtaatgc cgccttgcgc
    24301 agaaacggta tgggaacggg tcgggaagac tttagagagc agcgcacagg tctggccgct
    24361 ctgggaaatt tgcagcgccg cgcgcatacc tgcgccaccg gcaccaatca caactgcatc
    24421 aaattctctg actggcaatt tcatcacaca ccccacacca caacgaatcc ataaatcacg
    24481 taaaccacca gtgcaacgac aatcaccagt tgcagcatca ggcgcaaggc cagcggttta
    24541 acgtagtcgg tcaacacctg ccacatgccg atccaggcat gaatcaagat agaaaacagc
    24601 gccagcaggg tgaacacttt ggtgaacgca gaggcgaaga aaccgatcca gacttcatat
    24661 gtcagttcgc cactggtggc gaaaaaaccg accatataaa tgatgtagag cgtcaggacg
    24721 atagcggtag cgcgaacgag gatgaaatca tgtacgccat tgcgtcctaa tgcggaggcg
    24781 ttgcttacca tacgaggact cctgcgagaa gtgaaagcac gacagtaata acaaaggaga
    24841 ttttggcgga gcgtttaccc gcttcgaatg tttcttccag atagccaaaa tccatcatca
    24901 tgtggcgaat acctacgacg acgtgatacg ccagagcggt aaggatgccc cacatgataa
    24961 atttgacgaa gaagctgccc ataatcgcgg aagcttgctc gaaaccttca ggggaagaga
    25021 ggctggtacc cagaagccac agcaggatgc ccactgcaac aaaggtgatc acaccggaaa
    25081 cgcgatggag aatggacgct atcgccgtga tggggaaccg gatggtctgt aggtccagat
    25141 taacaggtct ttgttttttc acatttctta tcatgaataa cgcccacatg ctgttcttat
    25201 tattccctgg ggactacggg cacagaggtt aactttctgt tacctggaga cgtcgggatt
    25261 tccttcctcc ggtctgcttg cgggtcagac agcgtccttt ctataactgc gcgtcatgca
    25321 aaacactgct tccagatgcg aaaacgacac gttacaacgc tgggtggctc gggattgcag
    25381 ggtgttccgg agacctggcg gcagtatagg ctgttcacaa aatcattaca attaacctac
    25441 atatagtttg tcgggtttta tcctgaacag tgatccaggt cacgataaca acatttattt
    25501 aatttttaat catctaattt gacaatcatt caacaaaatt gttacaaaca ttaccaggaa
    25561 aagcatataa tgcgtaaaag ttatgaagtc ggtatttcac ctaagattaa cttatgtaac
    25621 agtgtggaag tattgaccaa ttcattcggg acagttatta gtggtagaca agtttaataa
    25681 ttcggattgc taagtacttg attcgccatt tattcgtcat caatggattc tttacctgca
    25741 agcgcccaga gctctgtacc caggttttcc cctctttcac agagcggcga gccaaataac
    25801 aaacgggtaa agccaggttg atgtgcgaag gcaaatttaa gttccggcag tcttacgcaa
    25861 taaggcgcta aggagacctt aaatggctga tacaaaagca aaactcaccc tcaacgggga
    25921 cacagctgtt gaactggatg tgctgaaagg cacgctgggt caagatgtta ttgatatccg
    25981 tactctcggt tcaaaaggtg tgttcacctt tgacccaggc ttcacttcaa ccgcatcctg
    26041 cgaatctaaa attactttta ttgatggtga tgaaggtatt ttgctgcacc gcggtttccc
    26101 gatcgatcag ctggcgaccg attctaacta cctggaagtt tgttacatcc tgctgaatgg
    26161 tgaaaaaccg actcaggaac agtatgacga atttaaaact acggtgaccc gtcataccat
    26221 gatccacgag cagattaccc gtctgttcca cgctttccgt cgcgactcac atccaatggc
    26281 agtcatgtgt ggtattaccg gcgcgctggc ggcgttctat cacgactcgc tggatgttaa
    26341 caatcctcgt catcgtgaaa ttgccgcgtt ccgcctgctg tcgaaaatgc cgaccatggc
    26401 cgcgatgtgt tacaagtatt ccattggtca gccatttgtt tatccgcgca acgatctctc
    26461 ctatgccggt aacttcctga atatgatgtt ctccacgccg tgcgaaccgt atgaagttaa
    26521 tccgattctg gaacgtgcta tggaccgtat tctgatcctg cacgctgacc atgaacagaa
    26581 cgcctctacc tccaccgtgc gtaccgctgg ctcttcgggt gcgaacccgt ttgcctgtat
    26641 cgcagcaggt attgcttcac tgtggggacc tgcgcacggt ggtgctaacg aagcggcgct
    26701 gaaaatgctg gaagaaatta gctccgttaa acacattccg gaatttgttc gtcgtgcgaa
    26761 agataaaaat gattctttcc gcctgatggg cttcggtcac cgcgtgtaca aaaattacga
    26821 cccgcgcgcc accgtaatgc gtgaaacctg ccatgaagtt ctgaaagagc tgggcaccaa
    26881 agatgacctg ctggaagtgg ctatggagct ggaaaacatc gcgctgaacg acccgtactt
    26941 tatcgagaag aaactgtacc cgaacgtcga tttctactct ggtatcatcc tgaaagcgat
    27001 gggtattccg tcttccatgt tcaccgtcat tttcgcaatg gcacgtaccg ttggctggat
    27061 cgcccactgg agcgaaatgc acagtgacgg tatgaagatt gcccgtccgc gtcagctgta
    27121 tacaggatat gaaaaacgcg actttaaaag cgatatcaag cgttaatggt tgattgctaa
    27181 gttgtaaata ttttaacccg ccgttcaaat ggcgggttaa tttttatatg cctaaacaca
    27241 aaaaattgta aaaataaaat ccactatcgg acattcatat attctaaaaa aaatatatcg
    27301 gtataaatta taaacaaatc gatgattttt aatcatctat cttcctctca gttacatgaa
    27361 taaaccacca aatatagcgc acccctataa tttccaatac tttattcatc aaacatttaa
    27421 ttcataccat caattaatta cttttacatt taataaaatt aataatattt ttaagaaaaa
    27481 ctaaaaacat tcgacaatca aataagatat tcttgcaccg atatactttt ttcttttctg
    27541 ttaaaggact cagttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
    27601 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
    27661 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
    27721 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
    27781 ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
    27841 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
    27901 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
    27961 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
    28021 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
    28081 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
    28141 tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt tatcgactct
    28201 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaatctga
    28261 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
    28321 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaatg
    28381 ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac
    28441 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
    28501 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
    28561 tgaatattag tattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
    28621 ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct
    28681 accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca gccggaatca
    28741 acacgggggc atggcgctta cgcagtgatt atcagttgaa tcatactgat agcgatgata
    28801 accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta ccgcaattag
    28861 gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac ggtttttctt
    28921 ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta cgcggctacg
    28981 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
    29041 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
    29101 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
    29161 aggtttcagc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
    29221 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
    29281 atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cagcggcctg ctgctttctg
    29341 gtgatgacta ccattctgcc gcaatgggta ttgggcaaaa tatgctgtgg cttggtgcgc
    29401 tgtcgtttga tgtcacgtgg gccagtagcc attttgatac tcagcaggac gagcggggct
    29461 taagctaccg ttttaattac agcaaacaag tggatgccac taacagcacg atttccctcg
    29521 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg gatcacaaat
    29581 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
    29641 ttaccccact aaacttcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
    29701 acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag
    29761 atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
    29821 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
    29881 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
    29941 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc tgacaatgga gcccaggtga
    30001 gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt acctatgccg
    30061 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
    30121 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
    30181 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
    30241 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggaagacgtt aacgtcatca
    30301 ttcgcaacgc cagcggtcag tttcctcccc tcggagcgga tatccgccag gatgacagcg
    30361 gcattagcgt ggggatggtt ggcgaggaag gacatgcctg gttaagcgga gtcgctgaaa
    30421 atcaaaaggt taccgtggtc tggggtgata gccagcattg ctcgctccat cttcctgaac
    30481 atatggaaga caccgcaaat cgcctgattt taccttgtca ttaattaagg aaatgaaaat
    30541 gacatttatg aaaggactac cccttttgtt gttggttgcc agtctgtgca gccacgctgc
    30601 actacaaccc gatcgcactc gtattgtgtt taacgctaat gataaagcta ccagtctgcg
    30661 ggtggataat cgtagcgata aactccccta cctcgcttat tcctggctcg aaaatgaaaa
    30721 aggggagaaa agcgatgatc ttctggtcgc cctgccgccg atacaacgtc tggaaccgaa
    30781 agcaacaacg caggtacgga tagtaaagca agcctcgacc acaaaattac cgggcgatcg
    30841 cgaaacgcta tttttctaca atatgcgtga aattcctccc gccccggaaa aaaacagcga
    30901 ccatgccgtt ctgcaagtcg ccattcagag tcgtatcaag gtgttctggc gaccggctgc
    30961 attacgcaag aaagcaggag aaaaggtcga actgcagtta caggtcagcc agcagggcaa
    31021 ccaactgaca ctgaaaaatc ctaccgcgta ttatctgacc attgcctacc tgggacgaaa
    31081 tgaaaaaggc gttctccccg gcttcaaaac cgtgatggtt gcgcctttta gtacggtcaa
    31141 cacaaacacc ggaaattata gcggcagcca gttttatctc ggttatatgg atgattacgg
    31201 cgcgttacgc atgacgacgc tcaactgcag cggacaatgc catttacaag ccgtggagac
    31261 gaagaaatga gtgctggcaa gggagtgttg ctcgtcatct gtctgttatt tctgccgttg
    31321 aagtctgcgc ttgccctgaa ctgctacttt ggttcatccg gtggctcggt agaaaaatca
    31381 gaagcgattc aaccttttgc tgtaccaggc aatgccaaac tcggcgataa gatctgggaa
    31441 tctgacgata ttaaaattcc cgtctattgt gacaacaata ccaacggcaa ctttgaaagc
    31501 gaacatgtct acgcatgggt aaacccctac ccgggggtgc aggatcgtta ttatcaactc
    31561 ggagtaactt acaatggcgt tgattatgac gccaaccagg gaaaaagccg catcgacact
    31621 aaccagtgta tcgacagtaa gaacattgac atttataccc ctgagcagat catcgcgatg
    31681 ggatggcaga acaaaatttg ctcgggtgat cccgctaata ttcacatgtc gcgtaccttt
    31741 ctcgctcgca tgcggttata cgtcaaaata cgagaaatgc cgccgcatga ttatcaaagt
    31801 acgcttagcg actatatcgt cgtgcaattt gacggtgccg gtagcgttaa cgaagaccct
    31861 actgcccaaa acctgaaata tcatattact ggtctggaaa acattcgcgt gctggattgc
    31921 agcgtcaatt tttccatttc accggaaaca caggtgattg attttggtaa atttaatttg
    31981 ctggatattc gtcgccacac aatgtcgaaa acattcagta ttaagacgac aaaatcacaa
    32041 aatgaccaat gcaccgacgg atttaaagtc agttcctcct tttataccga agaaacgttg
    32101 gttgaagaag ataaagcatt gttgattggt aatggtctga agctacgttt gctggatgaa
    32161 aacgcctcgc cttatacctt caataaatat gctgaatacg ccgatttcac cagcgacatg
    32221 ttggtctacg aaaaaaccta tacagctgaa ctttcgtcca ttgcaggtac ccccatcgag
    32281 gctgggccct tcgatacagt ggtgcttttt aaaattaact ataactgatt tgccatgatg
    32341 ccctttgcag ccggaaaggg catctctttc agaaaattgc tgattttccc cttttccttg
    32401 attgctgtta ttttttccta gcccggcttt ctttatgctg tatcgataca ccaaagagaa
    32461 aataatgagg gagcgaagga tgccagtttt gcagtgggga atgttatgtg tgctatcact
    32521 tctcctttct attggtttcc tcgcggtaca tctcccggcg gcgctattac ttgggccgat
    32581 gatcgccggg atcatcttca gtatgcgcgg aattactctg caacttcccc gctccgcttt
    32641 tctcgccgcg caggccattc ttggctgcat gattgcgcaa aacctcactg gttcgattct
    32701 caccacgctg gccgccaact ggccgatcgt gcttgctatt ttactggtga cgctgctttc
    32761 cagcgccatc gtgggttggt tattggtgcg ctatagctca ctgcctggaa ataccggtgc
    32821 ctggggttct tctccaggcg gcgcagcggc aatggtcgct atggcgcaag attacggcgc
    32881 agatattcgc ctggtggcgt ttatgcaata tctgcgagtg ctgttcgtcg cgggggccgc
    32941 tgttctggtt acccgcatga tgctgggtga taacgctgaa gcggttaacc agcaaatcgt
    33001 ctggttcccg cctgtgagca ttaatctcct gcttacgatt ttgctcgcag tcgttgccgg
    33061 tacggcggga tgcatgctgc gtcttccttc cggcacgatg ctcatcccga tgctggcggg
    33121 cgcagtgctc cagtctggtc agctcatcac catcgaacta ccggaatggc tgctggcgat
    33181 ggcgtatatg gcaattggct ggcggattgg tcttggtttc gataaacaaa tcttactgcg
    33241 ggcactacgc ccgctaccgc aaatcctgct gtcgattttt gctctgctgg ctatttgtgc
    33301 aggtatggcg tggggactga cccggtttat gcatattgat tttatgactg cctacctcgc
    33361 caccagtccc ggcgggcttg ataccgtggc ggtcatcgcc gcagggagca atgccgatat
    33421 ggcgcttatt atggcgatgc aaaccctgcg cctgttcagt attttgctga cggggccagc
    33481 cattgcgcgg tttatttcaa cctatgcgcc gaagcggtcg gcctagtgct ggcagcctgg
    33541 acaccagtaa aacgggcgcg aggacaacgt ggttttctca atgatgccgc cacagcgttc
    33601 gcacagttcg ccatcacgat gaaaaacctt aaagcgaaac agcgccccat gatgcttatt
    33661 ctcatccacc tgcccccgcg tagcgtaaga aaaccgagga atctccagta acgcgtgtgc
    33721 gagtgcatcc agttgcgccg cattgagatc tttcgcttta tgatttccag tcaacccaac
    33781 ctgccagagg atctccaccc gcaaataatt gccaagccca gccagaaacg cctgatcgag
    33841 cagtaatcct gcaaactgac ggttacgaaa gcgcggcgac aataaccgtt cctttaccac
    33901 ctccggcgtc agattcggat ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt
    33961 ggtcagttgt tccggcctca acatctcaat atccgaggcg ctataaagca gaatggtttt
    34021 gtcagccgtt tgcagtttta cccgcaatac tcgcgtggtc tgcggctctt cgccggtatc
    34081 aaccacgcgc cagacgccgt aaagctgatt atggctgtag agcgttaaac cgttgggaaa
    34141 atgagttaac aacgccttac cacgcgtttc cacatgggta acgtgttgac cgataagttg
    34201 tgattgataa gtttttaact gcgggaaggc aaaccagaca tcagttagtg gtttgccttt
    34261 gatcgccgcc tccaggttat ccgctgcacg gcggatctcc gggccttcag gcatggtgat
    34321 atcctttttt cattctttat taagcggggt tatgctgcga caacaatccc ctttttggca
    34381 aatgtagctc gtaatcggcg ggcgaacgcc agagcgtgct cgccgtcgcc atgcaggcag
    34441 accgtttgcg ccgcgaccgt tgcccattcg ccggtgatac ttttgactct gccgtgttgc
    34501 accatctcca gcgtttgcgc caacgcctgt tcttcgtttt caatcaacgc gcctggctga
    34561 cttcgcggca ccagcgagcc gtcagcctga taaccgcgat cggcaaacac ttcctcgcgc
    34621 gttgtcaggc catattgctt gcctgcacga atcagctcgc ttcctgccag cccaacgaga
    34681 accaatgccg gatcgcaagc gtataccgct ctggcgatgg cgtctgccag ttgtgcttct
    34741 ttcgccgcct ggttgtacaa catgccgtgc ggtttgacat gacgcattac gccgccttgc
    34801 gcacgggcaa tcgttgccag cgcgccaatt tgatacagcg tctgggcgta aacggtttcc
    34861 ggcggcagct gcatggcgct gcgaccaaaa ttttccctgt cgggaaaact cgggtgagcg
    34921 ccaatcgcga caccattttt tattgcttca cgcacgcaag cctgcatggt ttgcgcatcg
    34981 cctgcatgaa atccacaagc aatattggca gaggaaacca gcgttaatag ctctgcgtcg
    35041 ctggcgcagc cttcgcccag atcggcgttc aggtcaattt tcattgtgca gccgccacgc
    35101 taattgttca aaataacgtt gctgatcttg ccgcgctttc agtgcctctt ccagtgaaca
    35161 ctggacaaaa tgaatcggct gaccgagcgg aatttgcgcc agatggtaca tatcagcctc
    35221 aatgatacag gcaatacgtg gataaccacc ggtggtctgt gcgtcgttca tcaacacaat
    35281 cggctgcccg ttatgcggca cctgcaccac gcccggtaac aaaccgtgaa ataacagttc
    35341 gcgatcggtg atgcgtttta aaatttgccc ctgtaagcga tagcccattc ggttactttg
    35401 cgagctaagc tgccagggcg aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg
    35461 atattccggc cccggcaagg cgcgaatgcg gttgccccac agcagctgtt taacgccctg
    35521 cgcttccata aaatcacgct tcgatttgcc aatcgggagt cggtcaccat ccttcagtaa
    35581 acggccttcc agcccgccaa tccccacttt gagatcggtg ctgcatgacc ccattaccgg
    35641 cggaacatca ataccacccg caaccgccag ataactgcgc atcccgtgct gcgggcgttt
    35701 aagcgttaaa cgctggcctg ctttcatcgg caatcgccag ccggtccaga cggcgttatc
    35761 atccaaccgc gcttcgcaac ctgcacccgt cagggcaaac cacccatcag tttcgaactc
    35821 aactgttaac tgaccgagcg tgatctccag cgcgggggca ttggcgtcat tacccaccag
    35881 caggttagca atgcgcagcg caggcatatc cagtgcgcca cagtggctga tacccgactg
    35941 gcgaaaaccg tgacgaccgc catcctgcac agtggtgtac atgcccgcac gaataatctt
    36001 cagcatattc cctccttctg cggtacaaat cgcacgctgt ctcccggacg taataagatg
    36061 ggttcgtcac gcgccgaatc aaacaggctg agtgaggtat gaccaatcaa ctgccagcca
    36121 cccggcgttg ccagcggata aacaccagtc tgcggcccgc cgatcccgac agaacctgcc
    36181 ggaacgagca agcgcggttc agcgcgccgt ggcgtgtgta gttgttccgg caaactcccg
    36241 agatacggga agcccggttg aaaacctaaa aaccagacca cgtattccac ggaggagtgc
    36301 aattcaacaa cctgtttttc gctcaacccg caatacgccg cgaccaccgc caaatccggt
    36361 ccgcctgcac caccgtaaac caccggaatt tcaataaagc gagactcagg ttccagggcc
    36421 tcgctctcct cccaccagcg ttgcaaacgc tctatggcat ccagcgccag cgactcagga
    36481 ttacgcaaaa tcaccgtgat attgttcatg ccgggaatgg cttcaaccac attcggcata
    36541 tccaccagac gctgcgccag tcgccagatc cgtttctggc tagccagcgt caccggcggt
    36601 tccagttcca gcactaccgc cgtttcacct atcagataac aacgcgctcg ttgcactttc
    36661 gatccctctg attattcgtt atgcaggatt aggaatatca ataaaggtca catcaagatc
    36721 ggtattttca ttcagccact cgctcaatgc gcgaatacca ccgcgttcag tggcatggtg
    36781 acctgcagca taaaaatgca atccctgctc gcgggctgaa tgaatggtct gttcagaaac
    36841 ttcgccagta ataaaagcat ccacgccaaa acgcgcggcg ctatcgataa aactttgccc
    36901 gccgcccgtg caccaggcga cgcgctggac cacctcaggt ccggtatcgc cacaccataa
    36961 cggcttacgt cccagacgcg cttcaatcca ggaggctagt tccattcccg gcacaggcat
    37021 ggtcagctct ccccacggca ccaacggctc aatttcgccc atgaccgtga tccccagtaa
    37081 cgccgccagc tgtgcgttat tgcccagctc aggatgtgcg tcaagcggca aatgccagcc
    37141 atacaggttg atatcattcg ccagcaacgt ttttaaacgg ttacgcttca ttccgcgaat
    37201 gaccggagac tctcctttcc agaagtagcc atgatgcaca atgaccgcat ccgcgcccag
    37261 acgcactgcc tcatcgagca aagcctggct ggcggtgaca ccggtaacaa ttttttgcac
    37321 cgtctctttg ccttccacct gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt
    37381 caatttttcg ttaatcagtt gttccagttc ggtgtttttc atcttcgcgc cttccgtatt
    37441 ctgatagcta caccgttaac atagcgttac ctcgcctgtt cgtcgatagc caatcttttg
    37501 acatattaag aaacaaaccc gacgccaaaa gacgcaaatt gtttatatca tcacgcttca
    37561 gctaatgtat atttatgcaa tcagacagca tattgcataa ataatcagat ctgtatcaaa
    37621 gttttaactt taaggaaaca caagcacgcc gcgcaactat tacgtgacgt tgccgtcaat
    37681 tgtggaatta ttatgaataa acacgcatca caaccgcgcg ctatttacta tgtcgttgcg
    37741 ctgcaaatct gggaatattt tagcttttac ggcatgcgtg ccctgctgat tctctatctc
    37801 accaatcaac taaaatacaa cgatactcac gcctacgagt tatttagcgc ctactgttcg
    37861 ctggtgtatg tcacgccaat cctcggtggc tttttggcgg ataaagttct cggcaatcgc
    37921 atggcggtga tgctgggggc gttgttgatg gcgatcggtc atgtggtgct gggtgccagt
    37981 gagatccatc cgtcattcct ctatctgtcc ctggcgatta tcgtctgcgg ctatggcctg
    38041 tttaaatcta acgtaagctg tctgctcggc gagctgtatg agccaaccga tccgcgtcgt
    38101 gatggcggtt tctcgctgat gtatgcggcg ggtaacgtgg ggtctattat cgcacctatc
    38161 gcctgtggtt acgcccagga agagtacagc tgggcgatgg gctttggcct ggcggcggtt
    38221 ggcatgatcg cgggtctggt cattttctta tgtggcaatc gtcatttcac tcatacccgc
    38281 ggcgttaaca aaaaagtact gcgtgcgaca aactttctcc tgccgaactg gggatggctg
    38341 ctggttctgc tggtggcaac gcctgcactg attaccgtgc tgttctggaa agagtggtcg
    38401 gtatacgcct taattgtcgc gactattatt ggcctgggtg tactggcaaa aatttatcgc
    38461 aaagcagaaa accagaaaca gcggaaggag ctggggctga ttgtgacgct caccttcttc
    38521 agtatgttgt tctgggcctt cgcacaacag ggcggtagct cgattagcct ttatatcgac
    38581 cgcttcgtta accgcgatat gtttggttat accgttccga ccgcgatgtt ccagtcgatt
    38641 aatgccttcg cagttatgct gtgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc
    38701 gcaggtaatc gtaccgtgcg catctgggga aaatttgctc ttggccttgg cctgatgagc
    38761 gccggattct gcattctgac cttaagcgcc cgctggtccg caatgtatgg tcactcttct
    38821 ctgccactga tggtgttagg cctcgcggtg atgggctttg cggaactgtt tatcgacccg
    38881 gttgccatgt cgcaaattac gcgcattgaa attccggacg tgaccggcgt attaaccggc
    38941 atctacatgc tgctttctgg cgcgatcgcg aactatctgg cgggcgtgat tgccgatcag
    39001 acatcgcagg cttcgtttga tgcttccggg gcgatcaact actccatcaa tgcatatatt
    39061 gaagtatttg atcaaattac ctggggcgca ctggcgtgtg taggactggt actgatgatt
    39121 tggctgtatc aggcgctgaa attcagaaac cgcgcgctgg cgctggagtc ataataggcc
    39181 agcaccgatg cctgatgcga cgcttgacgc gtcttatcag gcctacgtat ttcctgcaat
    39241 ttattgaatt tgcacaaatt tgtaggctgg attaggcgtt cacgccgcat ccggcatctg
    39301 ggctcgattg cctgacgcgt ctgttatttc cccttccgcg ccgcctcata cgctgccaac
    39361 gtttgtattc tcgcctcttt gtgctcgact atcggttgcg gataatccag cttcacacct
    39421 gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc
    39481 ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt tctcgccctg agttatcgga
    39541 ttgaaaatac gaaaatacgg cgcagcatca gtacccgtag aagccgccca ctgccagcca
    39601 ccgttattag ctgccaaatc accatcaatc agctgcgaca tgaaatatcg ctcgccttcg
    39661 cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg tagccggtta
    39721 tgcatccagc cagtgctgtt aagctgacgc atagcggcat caataatcgg gtatcccgtt
    39781 ttgcctttct gccaggcctg taaatgtgcg ggattgcttt gccactgcac acgatccgtc
    39841 caggcaataa atggacgatg tttacacaac gaggggtaat acgtcatcag atgacggtag
    39901 aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc
    39961 ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc cgttgccaag
    40021 ctggcggaca aacggctggt gccttccact gccggaaaat ctcgttgttg ctcatactct
    40081 ccagcaccgt tctcgcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg
    40141 aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg cgcgggcttt
    40201 atcgatccgc tactacgaac ttttggtgca gcgacgcact ccggcatccc ttcccgcagc
    40261 cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg attgccggtc
    40321 atcaccgccc ccggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc
    40381 agcgctcttt caacgtccac atcccgcgct cgctcattca cctcatactg atagttataa
    40441 aaaagatgag taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca
    40501 aagtcatcca cttcacggaa caataaagga ataccttttt ccgccagcgc gatttgtagc
    40561 ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtctcccac
    40621 tggcgtggtg tagcgatata cagcgccagc acgc
//
LOCUS       DADLSB010000018        40184 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.018, whole genome shotgun sequence.
ACCESSION   DADLSB010000018 DADLSB010000000
VERSION     DADLSB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.018"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(1..523)
                     /locus_tag="JN331_000959"
                     /pseudo
     CDS             complement(1..523)
                     /locus_tag="JN331_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
     gene            complement(982..1068)
                     /locus_tag="JN331_000960"
     tRNA            complement(982..1068)
                     /locus_tag="JN331_000960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Leu,seq:gag)
     gene            complement(1083..1415)
                     /gene="secG"
                     /locus_tag="JN331_000961"
     CDS             complement(1083..1415)
                     /gene="secG"
                     /locus_tag="JN331_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY7259165.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLTTLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1643..2980)
                     /gene="glmM"
                     /locus_tag="JN331_000962"
     CDS             complement(1643..2980)
                     /gene="glmM"
                     /locus_tag="JN331_000962"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY7259166.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2973..3821)
                     /gene="folP"
                     /locus_tag="JN331_000963"
     CDS             complement(2973..3821)
                     /gene="folP"
                     /locus_tag="JN331_000963"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY7259167.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3911..5845)
                     /gene="ftsH"
                     /locus_tag="JN331_000964"
     CDS             complement(3911..5845)
                     /gene="ftsH"
                     /locus_tag="JN331_000964"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY7259168.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMPEQLGDK"
     gene            complement(5945..6574)
                     /gene="rlmE"
                     /locus_tag="JN331_000965"
     CDS             complement(5945..6574)
                     /gene="rlmE"
                     /locus_tag="JN331_000965"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY7259169.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6700..6993
                     /gene="yhbY"
                     /locus_tag="JN331_000966"
     CDS             6700..6993
                     /gene="yhbY"
                     /locus_tag="JN331_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY7259170.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(7149..7625)
                     /gene="greA"
                     /locus_tag="JN331_000967"
     CDS             complement(7149..7625)
                     /gene="greA"
                     /locus_tag="JN331_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY7259171.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7873..9306
                     /gene="dacB"
                     /locus_tag="JN331_000968"
     CDS             7873..9306
                     /gene="dacB"
                     /locus_tag="JN331_000968"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7259172.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9492..10652)
                     /gene="cgtA"
                     /locus_tag="JN331_000969"
     CDS             complement(9492..10652)
                     /gene="cgtA"
                     /locus_tag="JN331_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY7259173.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDGEDWDEDDEEGVEFIYKR"
     gene            complement(10668..11633)
                     /locus_tag="JN331_000970"
     CDS             complement(10668..11633)
                     /locus_tag="JN331_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7259174.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11760..12017)
                     /gene="rpmA"
                     /locus_tag="JN331_000971"
     CDS             complement(11760..12017)
                     /gene="rpmA"
                     /locus_tag="JN331_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY7259175.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(12038..12349)
                     /gene="rplU"
                     /locus_tag="JN331_000972"
     CDS             complement(12038..12349)
                     /gene="rplU"
                     /locus_tag="JN331_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY7259176.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12608..13579
                     /gene="ispB"
                     /locus_tag="JN331_000973"
     CDS             12608..13579
                     /gene="ispB"
                     /locus_tag="JN331_000973"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY7259177.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13808..14086
                     /gene="sfsB"
                     /locus_tag="JN331_000974"
     CDS             13808..14086
                     /gene="sfsB"
                     /locus_tag="JN331_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY7259178.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(14134..15393)
                     /gene="murA"
                     /locus_tag="JN331_000975"
     CDS             complement(14134..15393)
                     /gene="murA"
                     /locus_tag="JN331_000975"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY7259179.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(15448..15702)
                     /gene="ibaG"
                     /locus_tag="JN331_000976"
     CDS             complement(15448..15702)
                     /gene="ibaG"
                     /locus_tag="JN331_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY7259180.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15862..16155)
                     /gene="mlaB"
                     /locus_tag="JN331_000977"
     CDS             complement(15862..16155)
                     /gene="mlaB"
                     /locus_tag="JN331_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY7259181.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(16155..16790)
                     /gene="mlaC"
                     /locus_tag="JN331_000978"
     CDS             complement(16155..16790)
                     /gene="mlaC"
                     /locus_tag="JN331_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY7259182.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QVYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16809..17360)
                     /gene="mlaD"
                     /locus_tag="JN331_000979"
     CDS             complement(16809..17360)
                     /gene="mlaD"
                     /locus_tag="JN331_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY7259183.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17365..18147)
                     /gene="mlaE"
                     /locus_tag="JN331_000980"
     CDS             complement(17365..18147)
                     /gene="mlaE"
                     /locus_tag="JN331_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY7259184.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(18155..18964)
                     /gene="mlaF"
                     /locus_tag="JN331_000981"
     CDS             complement(18155..18964)
                     /gene="mlaF"
                     /locus_tag="JN331_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY7259185.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            19174..20151
                     /locus_tag="JN331_000982"
     CDS             19174..20151
                     /locus_tag="JN331_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY7259186.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            20165..21151
                     /gene="kdsD"
                     /locus_tag="JN331_000983"
     CDS             20165..21151
                     /gene="kdsD"
                     /locus_tag="JN331_000983"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY7259187.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRNALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            21172..21738
                     /gene="kdsC"
                     /locus_tag="JN331_000984"
     CDS             21172..21738
                     /gene="kdsC"
                     /locus_tag="JN331_000984"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY7259188.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21735..22310
                     /gene="lptC"
                     /locus_tag="JN331_000985"
     CDS             21735..22310
                     /gene="lptC"
                     /locus_tag="JN331_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY7259189.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            22279..22836
                     /gene="lptA"
                     /locus_tag="JN331_000986"
     CDS             22279..22836
                     /gene="lptA"
                     /locus_tag="JN331_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY7259190.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22843..23568
                     /gene="lptB"
                     /locus_tag="JN331_000987"
     CDS             22843..23568
                     /gene="lptB"
                     /locus_tag="JN331_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY7259191.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23616..25049
                     /gene="rpoN"
                     /locus_tag="JN331_000988"
     CDS             23616..25049
                     /gene="rpoN"
                     /locus_tag="JN331_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY7259192.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            25072..25359
                     /gene="hpf"
                     /locus_tag="JN331_000989"
     CDS             25072..25359
                     /gene="hpf"
                     /locus_tag="JN331_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY7259193.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25477..25968
                     /gene="ptsN"
                     /locus_tag="JN331_000990"
     CDS             25477..25968
                     /gene="ptsN"
                     /locus_tag="JN331_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY7259194.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTESTP
                     DEA"
     gene            26014..26868
                     /gene="rapZ"
                     /locus_tag="JN331_000991"
     CDS             26014..26868
                     /gene="rapZ"
                     /locus_tag="JN331_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY7259195.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26865..27137
                     /gene="npr"
                     /locus_tag="JN331_000992"
     CDS             26865..27137
                     /gene="npr"
                     /locus_tag="JN331_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY7259196.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27350..27982
                     /gene="yrbL"
                     /locus_tag="JN331_000993"
     CDS             27350..27982
                     /gene="yrbL"
                     /locus_tag="JN331_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY7259197.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27979..28707)
                     /gene="mtgA"
                     /locus_tag="JN331_000994"
     CDS             complement(27979..28707)
                     /gene="mtgA"
                     /locus_tag="JN331_000994"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY7259198.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGSEPFMQQHQLD"
     gene            complement(28704..29357)
                     /gene="elbB"
                     /locus_tag="JN331_000995"
     CDS             complement(28704..29357)
                     /gene="elbB"
                     /locus_tag="JN331_000995"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY7259199.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKELAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29587..31923)
                     /gene="arcB"
                     /locus_tag="JN331_000996"
     CDS             complement(29587..31923)
                     /gene="arcB"
                     /locus_tag="JN331_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY7259200.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(32019..32948)
                     /locus_tag="JN331_000997"
     CDS             complement(32019..32948)
                     /locus_tag="JN331_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY7259201.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGIRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            33623..38083
                     /gene="gltB"
                     /locus_tag="JN331_000998"
     CDS             33623..38083
                     /gene="gltB"
                     /locus_tag="JN331_000998"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY7259202.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDMTFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            38096..39514
                     /locus_tag="JN331_000999"
     CDS             38096..39514
                     /locus_tag="JN331_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7259203.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            39698..40177
                     /locus_tag="JN331_001000"
     CDS             39698..40177
                     /locus_tag="JN331_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY7259204.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT         9961 a        10847 c         9922 g         9454 t
ORIGIN      
        1 tggactgaac gcttttttat tttttgcatc tttataagca aattgacaag cggaaaataa
       61 actgcccgaa atgacaatca gcaataatat tcctgtcact ttttttgtcg gcaaggaaac
      121 atcatatttt attattactg ccaaaaaaag aagggataaa aaagcaaaag cacatagata
      181 agggatatac atccccagca ttttcacaac ttcatccgga tcactctgca gcacactaat
      241 cgcaaatcca tcattgaaag ttgtaccaaa ggtacaccat gaatagaggc taatactcat
      301 gtctgccgca gtcagtaaca caaatggtgc ggcaacaagc aggcgaagcg gaagggatga
      361 tatacgttta acaaccagga tggtcaggac gaagaacagc aatcgctgaa ccatatgcgc
      421 accggaagag gcgactaagt tcgtaatacc aaaaacaata acacaaagat acaacaacca
      481 atgagattta aaatcttcta gcaaatttgt tgaatactgt catccagaaa tccaagttca
      541 caaccaatat aaacaattat caacaatgtt caattttata tcagacatgt ttttcaactc
      601 tgatccatat cagaccgaaa cactgttagt tttttctttt taaatgatgc gaccattgcg
      661 ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac attacgcaga acgtagccat
      721 cagtagttat actgacagga gcaggatggt ttatcgagaa gtaaacatat ccgtattcgt
      781 accggaactg gagttgacac aataaagtgc caattatgtc agcagtaggg aaaagtttat
      841 aactaaggcg taccggcacc atcgtttcaa ggtaccagct acgagtaaag caactggacg
      901 agatacagat acctgacaac cattcatcag accaggacca aaacgaaaag agacgctttt
      961 cagcgtctct tttctggaat ttggtaccga ggacgggact tgaacccgta agccctattg
     1021 ggcactacca cctcaaggta gcgtgtctac caattccacc acctcggcac ggatactact
     1081 ttttagttcg ggatatcgct ggtcggctta gccggagcag ctggctgagt ttgttcggtt
     1141 ttcgccggtg cactcagatt ttcccattcg ctacctctat tggttttgtt gctattgatg
     1201 ttacccagca ccagactgat gatgaagaat aacgttgtca gcagcgccgt catgcgggtc
     1261 atgaagttac cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc tccgaaggag
     1321 gctcccatat cagcgccttt accttgctgc agcatgatca gaccaacaag gccaattgcc
     1381 acaataagga aaactactaa aagagcttcg tacataatca acctgttcct tgcggagttg
     1441 ccgcgtacca atgcttcaac caataaagcg ggagttttta atctttccca ctgaagcggg
     1501 tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa ttttatcgca ttgtatcaac
     1561 tgcggaaaaa aacagcaaaa ccgatgtgtt cgccgagaaa aaaggcgaca atcgccgcct
     1621 ttttagccag ttatctaacg ctttaaacgg cttttactgc atcggcgatg cggtgtgcaa
     1681 attcagtcac ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac ggttcggtgc
     1741 cggatttacg cagcaacacg cgtccacggt tgcccagcgc agcttcaacc tctgcggtca
     1801 cggctttaac tgactcatgc tcaagcggat cgccgctacc tgcggtgtaa cgtacgttaa
     1861 ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc atatggttac
     1921 gtgccatcgc cgccagcacc tgcaagccag caacgatgcc gtcaccggta gtggttttat
     1981 ccagcaggat cacatgaccg gaattctctg caccgatacg ccagcctttc tcctgcattt
     2041 tttccagtac gtagcggtca cccactttcg cgcgcgcaaa tggaattcct aactgtttca
     2101 gcgccagttc aagccccatg ttgctcatca atgtacccac agcgccacca cgcagctggc
     2161 cctgacgaag accttcacgc gcgatgatat acatgatctg atcgccatcg actttattgc
     2221 cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc gaaggcaata ccgagatccg
     2281 ctttttcagc cagcacacga gcctggagcg cgcgaacgtc ggtagccccc acttcggcat
     2341 tgatgtttac accgtttggc tcacaaccga tagcgataac gttcgccccc agttcgcgca
     2401 gcacgttcgg cgcgatgtga taagtcgcac cgtttgcaca atccaccaca atcttcagtt
     2461 cactgaggct aagttcgttc gggaacgtgg ctttgcaaaa ctcgatatag cgacccgcgg
     2521 catcaacgat acggctggct ttacccagtt ctgccgaatc aacgcagctg atctcctttt
     2581 ccatttccgc ttcgatggcc tcttctaccg catccggcag tttagtgccg tcgatagaga
     2641 agaatttaat cccattatcg tagaacgggt tatgcgatgc agatatcaca attccggcct
     2701 ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt tggcatcggg ccagtgaaga
     2761 gtgcggaaag gcccgctgcc gccagacccg cttccagtgc tgactccagc atatagccag
     2821 aaatacgcgt gtctttacca ataataatct tacgggagcc gtggcgcgcc agcactttac
     2881 ccgcggccca acccagctta agcacaaaat caggtgtgat cggcgcatcc cctacacgac
     2941 cacgaatccc atcggtaccg aaatatttac gattactcat agcgtttgtt ttcctttgca
     3001 gacagagtgg cttccaccac ccgcatcgct tctacggttt ctttgacgtc atgaacacga
     3061 atgatgtgcg cgccttgcat tgcggcaatg accgcacagg ccagactacc gctcaggcgc
     3121 tcggacggcc ccacgttcag cagctgccca atcatcgatt ttcgtgacat acccaccaac
     3181 agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata gttatgggag
     3241 agatttttac cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc gatacccgcc
     3301 tgctcgcaac gtgctatttg ctcaataaag tagcgattca cttctgcaaa gacatcgtca
     3361 tacttcggag cttcctgcat ggtttttgga tttccctgca tatgcatcag acaaaccggt
     3421 aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg aaagggagcg gatatcatta
     3481 ataatgtgag cgccaacttt cgctgactca cggatgactt ctggtttgga tgtatcgacc
     3541 gagatccaga cttcgaagcg ttgagcaatt gcctcaacca caggaataac acgttgcaac
     3601 tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg actcgccacc aacgtcaatg
     3661 atcgtcgcgc cagcattgat catcagattc gcatgtttca ccgcatctat cagcgagtta
     3721 tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga ggatccccat tacgtgagga
     3781 tggctaaggt ccagtgaagt accctgggca aagagtttca tgatgttatc cctggtatga
     3841 attgataaga aaaaaccccg gagcacgccc cggggttttc ggtacaaata cagtcatctg
     3901 atgcgggaac ttacttgtcg cctaactgct ctggcatggt gttacccggg ttcggcgtac
     3961 gcggttcatc aaccggacga ggagcctttg gactaccatt gtcgccagaa ttgttagaag
     4021 cgcctggttc ttcccagccc gctggcggac gtacatcgcg acgtgccatc aggtcatcaa
     4081 tctgcggtgc gtcgatagtc tcatatttca tgagagcatc tttcatcgca tgcagaatat
     4141 ccatattgtc ggtcagaagc tgacgcgcac gattatagtt acgctcaatc agtgctttca
     4201 cttcctggtc gatgatacgt gcagtttcat cggacatatg tttcgctttc gctacgctac
     4261 ggccgaggaa tacttcacct tcttcttccg cgtacagcag tggacccaat ttctcagaga
     4321 agccccactg agtcaccatg ttacgtgcca ggttggtcgc aactttaata tcgttggacg
     4381 caccggtaga aacatgttcc ggcccgtaga tgatctcttc tgccagacga ccaccgtaca
     4441 gcgtagaaat ctggctttcc agtttctgac ggctggcgct gattgcgtcg ccctcaggca
     4501 agaagaaagt cacacccagc gcacgaccgc gtgggataat cgtcacttta tgcaccggat
     4561 cgtgttccgg caccaggcga ccgataatcg catgacccgc ttcgtggtaa gccgttgatt
     4621 ctttctgcgc ttccgtcatc accatggagc gacgttccgc acccatcatg attttgtctt
     4681 tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt gccacgagca gcgaacagtg
     4741 ccgcttcgtt caccaggttc gctaggtcag caccggagaa accaggagta ccacgggcaa
     4801 tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg catgtgaact ttcaggatct
     4861 gctcacgacc gcgaacatct ggcaagccga ccacaacctg gcggtcgaaa cggccaggac
     4921 gcagcagggc cgggtcgaga acgtccggac ggttagtcgc ggcgataacg atgatacctt
     4981 cgttaccttc gaagccatcc atctcaacca gcatctggtt cagagtctgt tcacgttcat
     5041 cgtgaccacc gcccagacca gcgccacgct ggcggcctac ggcgtcgatt tcatcgataa
     5101 agatgatgca cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca cgggatgcac
     5161 ccacaccgac gaacatttct acgaagtcag aaccggagat agtaaagaac ggaactttcg
     5221 cttcgcctgc aatcgctttc gccagcaacg ttttaccggt acccggagga ccgaccatca
     5281 ggacgccttt cgggatctta ccgccgagtt tctggaagcg gctcggctcg cgcagatact
     5341 ctaccagttc agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca gcaaaggtcg
     5401 ttttgatctg atcttctgtc agcatgcgcg ctttgctctt accaaacgac atggcacctt
     5461 tgccaccgcc gccctgcatt tgacgcatga agaagatcca gacaccaatc agcaacagca
     5521 tcgggaacca ggagatgaag atagaagcca gcaggcttgg ttcttcaggc ggttcaccga
     5581 caaccttgac gttcttggtc aacaggttat ccagtaattt cggatcctga accggaatgt
     5641 aagtggtata acggttacta tctttcttgg taacgttgat ttcacgtccg ttgatacgcg
     5701 cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc accttacggc
     5761 cattagactc gctgggccca aagctctgga atactgacat cagcacaacg gcaatgacca
     5821 gccagagtat taggtttttc gccatgtcac tcaagggatt aacctcttat tacaactgtg
     5881 ttaaaaacag cgtcaggata ctctatatcc agcatctttc aaactttcgt ctgaaatctc
     5941 ccggttaggg tttacgcccg gtcgctacaa tatacacttc tcgcgaacgt gcacgagaag
     6001 agtccggctt acgaactttg accttcgtaa acagggagcg aatttccctt agatactcat
     6061 cgaaaccttc gccctggaac accttcacta caaaactgcc acctggcgct aatacatcac
     6121 gacacatttc tagcgccagt tccaccagat acatggcacg ggggatatcc accgccggtg
     6181 ttccgctcat gtttggtgcc atatcagaca tgacaacctg gactttgctg tcgccaacgc
     6241 gctccagcag tgctttcatc accagttcat cacgaaaatc gccctgaaga aagtccacac
     6301 caacgatagg atccataggt aaaagatcgc aagcgatgat gcggcctttg ccgccaattt
     6361 gggtgaccac atattgtgac caaccacccg gagcagcacc aaggtcgaca accgtcattc
     6421 ccggtttaaa gagtttgtca ctttgctgta tttcatcaag tttaaaccag gcacgggaac
     6481 gtaatccctt tttctgtgcc tgttgaacat atttatcgct aaagtgttcc tgaagccagc
     6541 ggctggagct ggcagaacgc ttcttacctg tcatttaact ttcccatggg gataactcat
     6601 cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga tatatgggag atggcggtag
     6661 aatgacccgt tttcaatccc aacgtaagca aaaaatacga tgaatctgag tactaaacaa
     6721 aaacagcacc tgaaaggtct ggcacatccg ctcaagccag ttgttctgct tggcagtaat
     6781 ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt tagagcacca tgaactcatc
     6841 aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct tgatcgtgga agctatcgtg
     6901 cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct ttatcgccca
     6961 actaaagaac gtaaaatctc gctgccacgc taagattatc ctaaagttac acacatttgc
     7021 tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc tgttcgttga
     7081 taaaaggccg cagagcggcc tttttccttt ctttacaata catcaacatc ttgagtattg
     7141 ggtaattctt acaggtattc caccttaatt acttcaaatt ctacttcgcc gcccggcgtt
     7201 ttgatgacca caacatcatc ttcttctttg ccgatcaggc cacgagcaat aggcgagttt
     7261 acagaaatca ggttttgttt aaagtcagct tcgtcatcgc caacgatgcg ataagtctgt
     7321 tcttcgtcag aatccagatt cagcaccgtt acggtagcac caaaaataac gcgcccattg
     7381 ttgggcattt tggtgacatc aatcacctgc gcgtttgaca gcttggcttc gatgtctttg
     7441 atacggcctt cgcagaagcc ctgctgttca cgagctgcgt ggtattcggc gttttctttc
     7501 aggtcgccat gctcacgcgc ttccgcgata gcagcaatga tttcaggacg gcgcacagat
     7561 ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc gtaaggtcat cggaatagct
     7621 tgcatttgtt atacctcttg aatattcctg atggggcaag tcttcaccca ccccggctgt
     7681 tcggcctgcc cggcataact cctcaccggg accagaagca aaaaaatacc gacccgggta
     7741 caagtcccag gtcagctaca attcacattt tgatggtcat tttaccctga agttcccgaa
     7801 gggtcatcgt ttactttata gggcgttgcg ccgtagtatg acggctcgat tccaggttgt
     7861 tagcgcgaga ttatgcgatt ttccagattt atcatcggat tgaccagctg tatagcgttc
     7921 agtgttcagg ccgcgaatgt tgatgagtac gttactcaac tccccgcagg tgccaacctt
     7981 gccctgatgg tgcaaaaagt cggtgcgtcg gcccccgcta ttgattacca cagtcagcag
     8041 atggcgctgc ccgccagtac ccagaaagtg attactgcgc tggcggcgtt gattcaactc
     8101 ggccccgatt ttcgtttcac cacgacgctt gaaaccaaag gcaatgtgga aaacggcgta
     8161 cttaagggtg acttagtggc gcgatttggt gccgatccga cgttaaaacg tcaggatatt
     8221 cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc aaatcgatgg caatgtgttg
     8281 atagatacct ccattttcgc cagccacgat aaagcccccg gctggccatg gaatgacatg
     8341 acacaatgct ttagcgctcc gcctgctgcc gccatagttg accgcaactg tttctccgtc
     8401 tcgctctaca gtgccccaaa gcctggtgat atggctttta tacgcgtggc atcttattac
     8461 cccgttacga tgttcagcca ggtacgcacc ctcccccgtg gttctgccga agcgcaatac
     8521 tgcgaactgg atgtggtgcc aggcgacctg aaccgcttta cgctgacggg atgcctgcca
     8581 caacgttctg agccgctccc gttggctttt gccgtgcagg atggagccag ctatgccggt
     8641 gcaattctga aagatgagtt aaaacaggcg ggtatcacct ggagcggaac actgctgcgc
     8701 cagactcagg ttaacgaacc tggaacggta gttgccagta aacagtcggc cccgctgcac
     8761 gatctgctta agattatgct gaaaaagtcg gacaacatga tcgccgatac ggttttccgc
     8821 atgatcggcc atgcgcgctt caatgtgcct ggaacatggc gggctgggtc ggacgccgtg
     8881 cgtcagatcc tgcgccagca agccggtgtc gatattggta acaccattat tgccgatggt
     8941 tcagggcttt cgcggcataa cctgattgcc cccgccacca tgatgcaggt gctgcaatac
     9001 attgcccaac acgacaatga acttaacttt atctccatgc tgccgcttgc gggctatgac
     9061 ggctctttgc agtaccgtgc aggtctgcat caggcgggcg tggatggcaa agtctcagcg
     9121 aaaaccggtt cgttgcaggg ggtatataac ctggcgggat tcattaccac agcgagcggg
     9181 caacgaatgg catttgtaca atatctttct ggctatgcag tagaacccgc ggatcagcgt
     9241 aatcgccgta ttccgttagt gcgttttgaa agccgtttgt acaaagatat ttatcagaac
     9301 aattagtcaa aaagaaaccc cggcgcatgg ccggggtttc agattattga caaagtgcgc
     9361 tttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaatcg tgcaaattca
     9421 atatattgca atcatcttgt aggcctgata agtgtagcgc atcaggctta tttggcattg
     9481 gtcattagtg attaacgctt gtaaatgaac tcaacgcctt cttcgtcgtc ttcgtcccag
     9541 tcttcaccat cctcttcagc aatctcttca agctgctgac ggtgataatc atcccacatg
     9601 aattcgactt tctctggctg tttcgcttct tcagcctgca cgaccgggtt ttcaatgata
     9661 aaggtcatca catcccagca gagatctttc acgcccagtc cgctcgccgc agagatcaga
     9721 taatatttat cttcccagcc cagcgcttca gcgatcgctt tcgctttctc ttcggcttct
     9781 gccttatcca gcagatcgat cttgttgaag actaaccaac gcggtttcgc cgccagatcc
     9841 tggctgtatt tttccagctc gctgataata atacgcgcgt tttcaaccgg atcggtgccg
     9901 tcaatcggat cgatatcgat gaggtgcaac aggacgcggc aacgttccag gtgcttcagg
     9961 aagcgaatgc ccagacctgc gccttccgca gcgccttcaa tcagtcctgg aatatcggca
    10021 acaacgaagc tcttttcgtt gtccattcgt accacaccca gacttggcac cagagtggta
    10081 aacggataat ccgccacttt cggtttagcc gccgataccg cacgaataaa ggtcgattta
    10141 cccgcgtttg gcatccccaa cataccgacg tcagccagca gcatcagctc cagcagcagc
    10201 tcacgcttat cgcccggcgt accgttagtt ttctgccgtg gtgtacggtt aactgacgat
    10261 ttgaaacggg tattgcccag accgtgccag ccgcccttag caaccagcag acgctgaccg
    10321 tgtttagtca tatcgcccat ggtttcacca gtaccctggt cgattacacg cgtacctacc
    10381 ggcactttaa tcgtcacgtc tttaccgcgc ttaccggtac aatcgcggct tgcgccgttc
    10441 tgaccgcgct ctgcacggaa agatttttca aaacgataat cgataagcgt gttcaggttc
    10501 tcgtcggctt ccatccatac gtcaccacca tcaccgccgt cgccgccatc cgggccgcct
    10561 ttcggaatat acttttcgcg gcggaagctc acgcaaccat taccgccatc acctgcaacg
    10621 accagaatcg atgcttcatc aacaaacttc attttattct ccgtaaatca ttcgcctgcg
    10681 cggggttgcg ataccaccgt tgtatgctta cgtaatccgc cccaaatacg atgaccaatg
    10741 gcggaataca tcgcgcccgc aaccacgaca aacgcaccga gataacctaa aaggtttaac
    10801 atcggtctgg cgaagaaatc gggccaggcc agtgataaaa gatctgaaaa aaacagcgta
    10861 aacagtgggg tgagcgtgat gatcgcgctc acctgcgctg cctgccagcg agccatcgct
    10921 tccgccaggg cgccatatcc taccaaggta ttcagtccgc aaaaaattaa acatgcgagc
    10981 tgccagtggc taagctgcgc tatcactcca ggctttgcca gagggaagag cgcgattgta
    11041 cataaagtgt acagtaaaaa caggatctgc ggtgaggcca gccgacgcaa taaaaccttt
    11101 tgcgccacgc cataactcac ccaaaccgtc gccgcaccga ccccaaagat aactccccag
    11161 gtgtaatcgg tgagctttgt aaatatctcg accagactgg tgttaaaaaa catcaccaga
    11221 ccgctcagga gcatcaatgc ccctacaacc tgagtgctgc gcattttctc tttcaggata
    11281 aatacgctgg caaccatcat gccaactggc gagagttgcc caatcacctg cgaagcggtc
    11341 ggactcaggt attgcaagga tgagctgaac aggatgaagt tcccaaacag cccggcggtc
    11401 gccactgcca acaaaatcaa ccagcgtggc ttacgaaaca cgcgtaatgg cggcaacctc
    11461 ttcttcaccg caagaatggc accaaggcca atactcgcca tcaagaaacg gtaaaacacg
    11521 attgtcggag gttccatcac ctccagcacc tgcttcattg cgattggcaa cgccccccag
    11581 caaattgctg tggtaagcgc caaaagaatg ccaatgcctg cctgctgctt cataccgtat
    11641 tccctgcaaa gcgcatttgc ggattaacgt cgcaccatgc gcatttttac cggttatcga
    11701 atgtaaaaag ccccgcaacg tgttgcgggg ctttcatccg ttaccgggac gcgaaaaact
    11761 tattcagctt cgatgctgat aaatttacgg tttttcgggc ctttaacttc gaatttcact
    11821 ttaccgtctg ctttagcaaa cagagtgtgg tcacgaccgc aacctacgtt agcgccagcg
    11881 tggaatttgg taccacgttg acgaacgatg atgctacccg ccagaacgga ttcgccaccg
    11941 aaacgcttaa cgcccaggcg tttagcttct gaatcgcgac cgttacgtgt ggagccgcca
    12001 gcctttttat gtgccatttg aaatctctcc tcaggtctta ggcgctgatg ccagtaattt
    12061 tcacatcagt gaaccactga cgatggccct gctgcttacg atagtgttta cgacgacgaa
    12121 acttaacgat tttaactttc tcgccacgac cgtgagcaac aacttcagct ttgattacgc
    12181 cgccatcaac gaaaggaacg ccgattttga cttcttcacc gtttgcgatc atcagcactt
    12241 cagcgaactc aacagtttcg ccagttgcga tgtccagctt ttccaggcga acggtctgac
    12301 cttcgcttac tcggtgttgt ttaccaccac tttggaaaac cgcgtacata taaaactccg
    12361 cttctgcgca caccttttca atgattcaga gtgcgctata aatattcaca atagggcgcg
    12421 aatattacgc aaaacgcacg cctttgacaa gtgctacagt caatacacga agaaaaaaaa
    12481 cacaacttgt acggtaacgt ttatctgtgc cattttttca gtacaattac cctatattcc
    12541 taaccataaa ccctaagttg cctttgttca cagtaaggta atcggggcga aaagcccggc
    12601 ttttgcgatg aatttagaaa aaatcaatga gttaaccgcg caagatatgg cgggtgttaa
    12661 tgcggcaatc cttgagcagc ttaattccga cgtccaactg atcaatcagt taggctatta
    12721 catcgtcagc ggcggcggta aacgtattcg tccgatgatt gctgtactgg ctgcacgagc
    12781 tgttggctat gagggaaatg cgcatgtcac cattgctgcc ctgatcgagt ttatccacac
    12841 ggcgactctg ctacacgacg acgttgtgga tgaatcagat atgcgcaggg gtaaagctac
    12901 cgccaacgcc gcatttggca atgccgccag cgtgctggta ggcgatttta tttatacccg
    12961 cgcgttccag atgatgacca gcctcggttc actcaaagtg ctggaagtca tgtcagaagc
    13021 tgtaaacgtc atcgcagaag gtgaagttct gcaactgatg aacgttaacg atccggacat
    13081 cactgaagaa aactacatgc gcgttatcta tagcaaaacc gcgcgtctgt ttgaggctgc
    13141 cgcgcagtgt tccgggattc tggctggctg tacgccggag gaggagaaag gcctgcagga
    13201 ttatgggcgc tatctcggca ctgctttcca gttgatcgac gatttactcg attacaatgc
    13261 cgatggcgaa cagttaggta aaaacgtcgg cgacgatctg aacgaaggta aaccgacgct
    13321 gccgctgctg catgcgatgc atcatggcac accagaacag gcacagatga tccgtaccgc
    13381 catcgaacag ggtaacggtc gccatcttct ggaaccggtt ctggaagcaa tgaacgcttg
    13441 tggatctctt gaatggacgc gtcagcgcgc cgaggaagaa gcagacaaag ccatcgcagc
    13501 gttacaggtg ctcccggaca ccccttggcg agaagcactc atcggcctcg cgcacatcgc
    13561 tgttcaacgc gatcgttaat cccctccctt catcccgcgc agtgcgcggg atgattccag
    13621 taaattccat aaaaacacta attcagcgct ataacacctg aaaaaaaagg gcgatgaatc
    13681 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat aggccatcag
    13741 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    13801 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    13861 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    13921 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    13981 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    14041 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    14101 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    14161 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    14221 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    14281 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    14341 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    14401 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    14461 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    14521 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    14581 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    14641 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    14701 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    14761 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    14821 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    14881 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    14941 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    15001 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    15061 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    15121 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    15181 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    15241 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    15301 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    15361 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    15421 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    15481 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    15541 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    15601 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    15661 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    15721 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    15781 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    15841 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    15901 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    15961 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    16021 gctaagatcg atgcaggtaa tccccttcac cgcttcctca cgcatttccc aaagcggtag
    16081 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    16141 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    16201 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    16261 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    16321 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    16381 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    16441 taggtttgac cgtgatacat cgccagcgcc tgaccgtaaa cctgcttcag gtactcacgg
    16501 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    16561 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    16621 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    16681 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    16741 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    16801 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    16861 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    16921 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    16981 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    17041 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    17101 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    17161 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    17221 tcgaacgtcg cataaagcgt gtaggtcggt tcagtacgta tggacgtcac gttcgccgcc
    17281 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    17341 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    17401 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    17461 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    17521 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    17581 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    17641 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    17701 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    17761 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    17821 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    17881 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    17941 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    18001 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    18061 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    18121 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    18181 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    18241 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    18301 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    18361 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    18421 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    18481 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    18541 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    18601 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    18661 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    18721 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    18781 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    18841 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    18901 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    18961 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    19021 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    19081 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    19141 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    19201 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    19261 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    19321 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    19381 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    19441 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    19501 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    19561 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    19621 gctgaacgtc aggggactga cagcctgacc agagagcagc ttgcagagct gccgcgtgac
    19681 ggcggattgc ccgtcgcgtt tttatggctc ggcattgcgc ttatcatcat gccagtggcc
    19741 acgcggatgg tggttgataa cgccacggtg ctggcgaatt actttgccat cagcgagttg
    19801 acgatgggtc tgacggcaat tgctatcgga accagtctgc cggaactggc aaccgcaata
    19861 gcgggggttc gcaaaggtga aaacgacatt gctgtcggaa atatcattgg cgcaaacatt
    19921 tttaatattg tcatcgtgtt gggtttaccc gcgctgataa cgccaggaga gattgatcca
    19981 ctggcgtaca gtcgtgacta cagcgtgatg ttgctggtga gcattatttt tgcgttgctg
    20041 tgctggcggc gctccccgca accgggccgt ggtgtagggg tattattaac tggcggattt
    20101 atcgtatggc tggcgatgtt gtactggtta tcgccaatac tcgttgaata actggaaacg
    20161 cattatgtcg cacgtagagt tacaaccggg ttttgacttt cagcaagcag gtaaagaagt
    20221 cctggcgatt gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc agaatttcac
    20281 gcttgcctgt gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa
    20341 atcggggcac atcgggcgca aaatggccgc aacgtttgcc agcaccggta caccttcatt
    20401 tttcgtccat cctggtgaag ccgcgcatgg tgatttaggc atggtcaccc cacaggatgt
    20461 ggtgattgct atctctaact caggtgaatc cagcgaaatc acggccttaa ttccagtgct
    20521 taagcgtctt cacgtaccgt taatctgcat caccggtcgc ccggagagca gcatggcgcg
    20581 cgccgcagat gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc
    20641 accgaccagc agcaccaccg ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt
    20701 aaaagcacgc ggctttactg ctgaagattt tgcgctctca cacccaggcg gcgcactggg
    20761 tcgtaaactt ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga tcccgcatgt
    20821 taagaaaacg gccagtctgc gtaacgcatt gctggaagtt acccgcaaaa atcttggtat
    20881 gactgtcatt tgcgatgaca atatgatgat tgaaggcatc tttaccgacg gtgatttacg
    20941 ccgtgtcttc gatatgggcg tggatgttcg tcagttaagt attgccgatg tgatgacgcc
    21001 ggggggaata cgtgtacgcc ctggcattct ggccgttgag gcactgaact taatgcagtc
    21061 ccgccatatc acctccgtga tggttgccga tggcgaccat ttactcggtg tgttacatat
    21121 gcatgattta ctgcgtgcag gcgtagtgta aagattcaag gataaacaac aatgagcaaa
    21181 gcaggtgcgt cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat ggcaaaagca
    21241 gagaacattc gtctgctgat cctcgacgtt gatggcgtac tgtcagatgg cctgatttat
    21301 atgggcaata acggcgaaga gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt
    21361 tgtgcgctca cctctgatat tgaagtcgct atcattaccg ggcgaaaggc taaactggta
    21421 gaagatcgtt gtgccacatt ggggatcact cacttgtatc aggggcagtc aaacaaactg
    21481 atcgccttta gcgatttgct ggaaaaattg gcgattgccc cggaaaatgt ggcttatgtt
    21541 ggcgatgatc tcatcgactg gccggtaatg gaaaaagtgg gtttaagcgt cgccgtggcc
    21601 gatgcgcatc cactgttgat cccgcgcgcc gattacgtga cgcgcattgc tggcggtcgt
    21661 ggcgcagtgc gcgaagtttg cgacttatta ctcctggcgc agggcaaact ggatgaagcc
    21721 aaagggcaat cgatatgagt aaagccagac gttgggttat cattgtgcta tcactggcgg
    21781 ttctggtgat gatcggcatt aatatggccg aaaaagacga taccgcccag gtggtcgtca
    21841 acaacaatga tcccacctat aaaagcgagc atacggacac gctcgtctat aacccagaag
    21901 gggcactaag ctatcgattg attgctcaac acgttgaata ttattccgat caggccgttt
    21961 cgtggtttac gcagccggta cttaccacgt ttgataagga taaaatcccg acatggtccg
    22021 taaaagcaga taaagccaag ctgaccaatg accggatgct ctatttatat ggacacgttg
    22081 aagtcaacgc acttgtgcca gactctcaac ttcgcagaat cacgacggat aacgcgcaga
    22141 tcaatctggt gacacaggat gttacctctg aagacctcgt cacgttatac ggaacaacat
    22201 ttaactccag cggtctgaaa atgcgcggca acttacgcag caagaacgcc gagctgattg
    22261 aaaaggttag aacatcctat gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt
    22321 gccagctcac ttctggccgc cagcattccg gcatttgccg taaccggaga cactgatcag
    22381 ccgatccaca ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt ggttaccttt
    22441 accggtaatg tcatcgtcac ccagggcacc atcaaaatta atgccgacaa agtggtcgtt
    22501 acccgtccgg gcggcgaaca aggtaaagaa gtgattgacg gatacggtaa accggcaacg
    22561 ttctaccaga tgcaggacaa cggtaaaccc gttgaaggtc acgcttccca gatgcactac
    22621 gaactggcaa aagattttgt cgttctgacg ggtaatgctt atctgcagca ggtcgatagc
    22681 aacattaagg gcgataagat cacttacctg gtgaaagagc agaaaatgca ggctttcagc
    22741 gacaaaggca agcgcgtaac caccgttctg gtgccgtcgc agctgcagga caaaaacaac
    22801 aaaggccaga ccccggcaca gaagaagggt aattaattcg ttatggcaac attaactgca
    22861 aagaaccttg caaaagccta taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc
    22921 aactccgggg aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac caccactttc
    22981 tacatggttg taggcattgt gccgcgcgat gcgggcaaca tcattattga tgatgacgat
    23041 atcagtctgc tgcctctgca tgcacgcgcg cgccgcggta tcggctatct gccacaggaa
    23101 gcctccattt tccgtcgcct cagcgtttac gataacctga tggcggtact gcaaattcgt
    23161 gacgacttgt ctgctgaaca acgtgaagac cgcgcgaacg agctgatgga agagtttcac
    23221 attgagcacc tgcgtgacag catggggcag tcactctccg ggggtgaacg tcgccgtgta
    23281 gaaattgccc gcgcactggc tgcgaatccg aaatttattc tgctcgacga accgtttgcc
    23341 ggggttgacc cgatctcggt tatcgacatt aaacgcatca ttgaacacct gcgcgacagc
    23401 ggcctgggcg tgctgatcac tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc
    23461 gcttatatcg tcagtcaggg gcatttgatc gcccacggca cgcctacaga aatcttacaa
    23521 gacgaacacg ttaagcgtgt ataccttggg gaagacttca gactctgata gggtagaagt
    23581 ttgcgacgtt ttagcaggag agtacgattc tgaacatgaa gcaaggtttg caactcaggc
    23641 ttagccaaca actggcgatg acgccacagc tccaacaggc aattcgtctg ttgcagttgt
    23701 cgacgctgga acttcagcag gagctacagc aggcgctgga gagtaatccg ctgcttgagc
    23761 aaatcgacac tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa acgctggaca
    23821 ccgccgacgc gctcgaacaa aaagagatgc cggaagagct gccgctcgat gccagttggg
    23881 acaccattta caccgctggt acaccatccg gcaccagcgg tgactacatt gacgacgagc
    23941 tgccggtcta ccagggcgaa acgacgcaga ccttgcagga ttacctgatg tggcaggtcg
    24001 agctgacacc gttttccgac actgaccgcg ctattgctac ctctatcgtc gatgccgttg
    24061 atgaaaccgg ttatctgact gtcccgctgg aagatattct cgaaagtatg ggcgatgaag
    24121 agatcgacat cgacgaggtt gaagccgtcc ttaagcggat ccaacggttt gatccggtcg
    24181 gtgtagcggc aaaagatctg cgtgactgcc tgctaatcca actctcccaa ttcgataaga
    24241 ccacgccgtg gctggaagag gccagattga tcattagcga tcatctcgat ctgttagcca
    24301 atcacgactt ccgcacttta atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag
    24361 ccgtcaatct gatccagtcg ctcgatccgc gccccgggca gtcgatccag actggcgaac
    24421 ctgagtatgt cattccagat gtgctggtgc gtaagcataa cggtcactgg acggtagaac
    24481 tcaacagtga cagcattccg cgtctgcaaa tcaaccagca ctacgcctcg atgtgcaata
    24541 acgcgcgcaa cgatggtgac agccagttta tccgcagcaa tctgcaggat gccaaatggt
    24601 tgatcaagag tctggaaagc cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg
    24661 aacagcagca agccttcttt gagcaaggtg aagaatatat gaaaccgatg gtactggccg
    24721 atatcgccca ggctgtcgaa atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat
    24781 acctgcatag tccacgaggc atttttgaac tgaagtattt cttttccagt cacgtcaata
    24841 ccgagggcgg cggcgaagct tcctccacgg cgattcgtgc actggtgaag aaattaatcg
    24901 cggcggaaaa cccagcgaaa ccgttgagcg acagcaagtt aacctctttg ctgtcggaac
    24961 aaggtatcat ggtggcacgc cgcactgttg cgaagtaccg agagtcttta tccattccgc
    25021 cgtcaaacca gcgtaaacag ctcgtttgac ccaaccgata aggaagacac tatgcagctc
    25081 aacattaccg gaaataacgt cgagatcacc gaggcactgc gcgaatttgt tacagctaaa
    25141 tttgccaaac ttgagcaata ttttgaccga atcaaccagg tctatgttgt tctgaaagtg
    25201 gagaaagtca cccacacctc agatgcaaca ctgcatgtaa acggtggtga aattcatgcc
    25261 agcgcggaag gtcaggacat gtacgctgcc attgatggct taattgataa gctggcacgc
    25321 cagttgacca aacataaaga taaactgaaa caacactaat tgtccgggca attagcatgt
    25381 gcatggcggc ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct ccgagcctgt
    25441 tccactgttt gagtgggcag gttcttaggt gaaattatga caaataatga tacaactcta
    25501 cagcttagca gtgttcttaa cagggaatgt acgcgaagcc gcgtccactg tcagagcaaa
    25561 aaacgcgccc tggaaatcat cagcgagctg gcggcgaaac aacttagcct gccgcctcag
    25621 gtggtttttg aagctatcct gacgcgggaa aaaatgggca gtaccggtat cggcaatggt
    25681 attgccattc cgcatggcaa acttgaagaa gatactctgc gtgccgttgg cgttttcgtt
    25741 cagctcgaaa cgcctatagc tttcgatgcc atcgacaacc aaccggtgga cttacttttt
    25801 gccctgctgg tgccggcaga ccaaactaaa acgcacctgc atactctgtc gctggtggcg
    25861 aaacgtctgg cggacaaaac catctgccgc cgtttacgcg cagcccagag cgatgaagag
    25921 ctgtatcaaa tcattacgga taccgaaagt actccggatg aagcgtagtt attcggtaat
    25981 gtctctttta gacgttgtga ggagaaacag tacatggtac tgatgatcgt cagcggacgt
    26041 tcaggttcag gtaaatctgt cgccctgcgt gcgctggaag atatgggttt ttactgcgtg
    26101 gataaccttc ccgtagtgtt gttacccgat ctggctcgaa ctctggccga tcgcgagatt
    26161 tctgccgccg tcagcattga tgttcgtaat atgccggagt caccagaaat attcgaacag
    26221 gcgatgagta acctgcctga cgctttctca ccgcaactac tgttcctgga tgccgaccgt
    26281 aataccttaa ttcgtcgtta cagtgacacg cgccgactgc atccgctttc cagcaaaaac
    26341 ctgtcgctgg aaagtgctat cgacaaagaa agcgatttgc tggagcctct gcgttcgcga
    26401 gcggatctga ttgtcgatac ctcagaaatg tccgttcacg agctggctga aatgctgcgt
    26461 acccgtctgc tgggtaaacg tgaacgcgaa ctgaccatgg tctttgagtc tttcggcttc
    26521 aaacacggta tccctatcga tgcagattac gtctttgacg tgcgcttctt gccgaacccg
    26581 cactgggatc cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc cgcgttcctc
    26641 gaccgccaca cagaagtaca caattttatc taccagacgc gaagctatct tgagctatgg
    26701 ttacctatgc tggaaaccaa caaccgtagc tacctgacgg tcgccattgg ttgtaccggc
    26761 gggaagcacc gttcggtgta tattgcagag caactggcag actacttccg ctcgcgcggt
    26821 aaaaacgtcc agtcacgcca tcgtacgctg gaaaaacgta aaccatgacc gtcaagcaaa
    26881 ctgttgaaat cacaaacaag ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat
    26941 taatgcaggg ttttgacgct gaagtgctgt tacgtaatga cgaaggcacc gaggctgaag
    27001 ccaacagcgt tattgcgctg ctgatgttgg attctgccaa aggacggcag attgaagttg
    27061 aagcgaccgg tccacaggaa gaggaagcac tggccgccgt tatcgccctc tttaattctg
    27121 gttttgatga agattaatct tcatcacttt gacatacaac tcccttcaag ctcccccgac
    27181 aataagaaaa tcacgtactg aaatcgttct caatcaacgt catttgtaca ttttgtgcgc
    27241 ttttcacttt cagaagaacc ttaagaaaac cttaagaggc attgtttagg ttttgtttaa
    27301 gttaatcgat catactggag atcgtcagaa aatatttcca ggagatggca tgattcgctt
    27361 atctgaacaa agtcccctgg gcaccggaag acatcggaaa tgttatgcgc atccggaaga
    27421 tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata aagagatccg
    27481 ccgtgagtta aagtactacg cgcatcttgg tcgccggtta aaagactgga gtggaatacc
    27541 gcgctatcac ggtaccgttg agacggattg cggaaccggt tatgtctacg atgtgattgc
    27601 cgattttgac ggcaagcctt ccattacgct gaccgaattt gctgaacaat gccgttacga
    27661 agaagacatt gcacaactgc gccagctact gaaacagctg aagcgttatt tgcaggataa
    27721 ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc
    27781 agaggttatc ccggtcgtct gcgacaacat tggtgaaagc acattgattc cactggcgac
    27841 ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta tggaaacgat ttatcgccca
    27901 accggcgctg gctattgccc tgcagaaaga tctgcaacct cgtgagagca aaacgctggc
    27961 cctcacttcg cgcgaagctt aatccagctg gtgttgttgc ataaacggct cactgcctaa
    28021 ctgatacatc tgccgtaaaa tccacgcctg acggctacgc acgtagcccg atggcgagga
    28081 gactttgaaa cgaagtggat taggtaatac agctgccagt aatgcagctt ccgaccgggt
    28141 aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc caaacacacc
    28201 gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata cgctttttgc tccagactgt
    28261 ttctatcccc agcgttaatc cagcttccag cccttttcgt acccagctac gcccatccca
    28321 taaaaagaga tttttggccg tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc
    28381 attgcgctcg ttgtgcgcca gggctttctc aatggaagcg acatcaaagc cccagtgctc
    28441 aggaaatttc tgatcttctg cggcaatcac cgccagtccc atccacggcg agatttgatc
    28501 catactgacc cagtcagaat gtgccacgta acgaaaattg ccatgcagcc aggcgctgac
    28561 ctgtcgctcg accattaccg ctgagaaggg aacaggcgca acgctaaaca acgcgatgcc
    28621 cccgccccag aaaacggcga ggacaaccat taaccgcaaa agaaaacggc gaacgaaact
    28681 aaacaccgtt aagcggcttt tactcattca gccagaacca gcacgcggga aaccagctta
    28741 tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca tatatgctgg ggtggtgaca
    28801 atcttattgt cttcatcaac cacgatatca tcgacaggac acggcacatg ctccgcgccc
    28861 atctcttcca gcacttctgc ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa
    28921 tcgaaaattt tcggcagcat cgccggggca atacacataa aaccaagcgg ttttccggcc
    28981 tgatgcatcg cttgtgccag ctcctttaat tcacggtcaa cggtgcattc gctaccaaga
    29041 ctggcaaaat tgcttaaatt cttcgccgcg ccaaaccccc ccggcacaat caacgcatcc
    29101 agttcagcgg catcggcctg ggccagagga cggatttcac cgcgcgttat tcgtgccgct
    29161 tcaatcagca cattgcgcgt ttccgtcatc gcttcgccag ttaaatggtt gataacatca
    29221 acctgctgct tatccggtgc aaagcagacc gcctgcgcac cgctgcgtga aatagctaac
    29281 aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga cgccgcatcc gctcagaatt
    29341 acgccaattt tcttcattgt gatcatcctt tcgcaattga ctgaaacaca ttaatttttt
    29401 taataaaaat ggtacgcatc acacatttaa ctgattcatg taacaaatca tttaagtttt
    29461 gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc ccttgtaact catcataata
    29521 atttacggcg cagccaagat ttccctggtg ttggcgcagt attcgcgcac cccggtctag
    29581 ccggggtcat tttttagtgg cttttgccac ccacgctttc agcacttcta cgtcgtgacg
    29641 ccactcttct ttcatctctt caatccattc accgacgtta tcttcccagg cgggaaggtc
    29701 aggagactga atttgctgac ccagctgttg cagatggcgt aaccccactg accccgccgc
    29761 acctttaatt ttatgtcctt cctcaacaat gcctttttta tcctgcgccg tcagattcga
    29821 ctccagcacg ctgacatagc ccggcatcat cttctcaaac accgctaacc cgtcggtgat
    29881 cagcttcggt cctacaagtt cgagatactg ttccagcatg ggaatatcga gcaatgcttc
    29941 tgatttactg ttctcttctg tcgtcaccgt actctcctca tcatcctggg tatcccagaa
    30001 tttcttgatc atcgcggtta gcgccggaac cgaaagcggc ttactcagca catcatccat
    30061 tccagcattg aggtactctt gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg
    30121 cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga gagatatcca gcccggtcat
    30181 atctggcaac tgaatatcca gcaacaccag gtcgtattcg cccggtttaa acatctccag
    30241 cgccgccttg ccggtcatgg cgacatcaac gctgttacct aatttttcca gcacagaacg
    30301 cgcgacaatc acgttcagtt caatgtcttc caccagcagc acattcagcg ccggtaaagg
    30361 catatcgtct tcatcaaacg catcatcgac ctcttctgcc accgacggtg cgtggatcgt
    30421 caacgtaaag gttgaacctt tgccctgttc gctggtaacc gtaatatcgc cacccatatt
    30481 tttcgccaga cgacgagaaa cggccagacc aataccggtg ccggtggcag gtttaccgcc
    30541 atgactgtct ttcacctggt aatacatggc gaagatttta tccagctcat cctgcggaat
    30601 gccgatacca gaatcttcca cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg
    30661 cacggtaacc tggccttgct gggtgaattt gacggcgtta ctgatgaggt tccacaggat
    30721 ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt aatggcagcg taggctccag
    30781 gttaaagcgc aatccttttt gttgcgcctg caatgcggag agattttcca gatcggcaag
    30841 gaagctggtg aaatcaaccg gctgattatc aagctggacc ttgcgccgtt ccatcttatc
    30901 catgtcgata atatcgttaa agatattccc cagcgtgacg gccgaaacat ggatagtttt
    30961 gagatatttt tcctgctcgg cggtgagttc ggtatccagc agaatgcggc tcaggccgac
    31021 gataccgttc agcggtgtac gcaattcgtg actgatggtg gaaataaacg tcgttttgtc
    31081 acggctggcg cgttcaagcg catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa
    31141 gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac ggcactttac ggatttcaaa
    31201 gcaggctttg cgcccgtccg ggtaatccag ccactgttca taggtcagtg acacattatg
    31261 acggaacact ttttcatcgg tttcaatgac ttttgcggcg gcttccggtg agtaaacatc
    31321 agcaggtttc aggtgaacca gttgtttttc gctttttccg gtcagcagct ccatcgcgcg
    31381 gttacagccg gaaaactctt tatcttcgtt acgataaaaa accaggtcgg gtgaagcatc
    31441 aaggaaggaa cgtaagaatg aggattgctg ctcgagctga atttgtgtct cttcgcgctc
    31501 tttgatttca attttcagtt ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg
    31561 aacggcaatt tcctgattta gctgggcaat attatctttt aactgaacgt tgaggctcaa
    31621 atcgcgctcg cgcatctcct ccagtttttg caccagccgt gacagacgtt gtcgtgactc
    31681 ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc
    31741 aaagaagata gaacgaataa catcaatgct ttcgacctga ccatgcagca ccatggttac
    31801 cgccatttgt accacaatgg caagaacgac gagggccagc gccagcaaca ttgagaagcg
    31861 caccagacct aacttcatca tcaggtcaac ataatactgc gccagcagac gaatttgctt
    31921 cattagggaa ttccttcacg acaacctgcc ccaataatac ccaattctga cagttacgtt
    31981 ataaattgtg tgagaaatgc ggaattcccc tcaacgacct actccgttgg aggtagccac
    32041 ggacgtccca gcgccgatcc ctgtacgcca tgttcattca gatagcgatc cagctcgacc
    32101 attcccgtcc atcgattttc gcaccacagc ggagcaagca gcgtcgggcg acgggcgctg
    32161 gcggaaatgc ggtggtagat cacttccggt ggcgtatggc gaatcatttc tccggcagtg
    32221 agcgtataat cctccagttc aataccgttc aaacgccccg cttcccaggc tttcgccata
    32281 atgctgcctt tcacaatatg cagcggatgc agctttatgc cgtccacgcc ggtttcaacc
    32341 actcgctcca gcgtttgcaa acattcagcc tgcccttcgc caggcaggcc gacaattaag
    32401 tgggaacaca ccttcagccc gcgctgacgt gccagctggg ttgtacgctg ataacaggca
    32461 aaatcatggc cgcggttgat gcgatgcagt gttttgtcgt gggcggtttg tagccccagc
    32521 tccagccaca cttcgtaacc ctggtcctta tattcgcaaa gcagatccag caccgcatcc
    32581 ggcacgcagt ccgggcggat accaacacac aaaccgacaa tattggcctg gctcaccgcc
    32641 tgctgataca tagaacgcag cacctgaact tccgcaaagg tgctggtata cgcctgaaag
    32701 taggccagat agcgtttagc gcggttaact aaattcgcct ggtgcgccag ttgctcggca
    32761 atggaacgat gctgctgcgc ttcatcggca aacgaggcaa cattacagaa tgtgcagcca
    32821 ccacgcccga tggtaccgtc acggttaggg cagctaaaac cgccgtgcag cgtcagctta
    32881 tgcacctttt gcccataacg acgggtgaga tcaccaccaa acatattgac taatttctgt
    32941 aactgcataa tctgatagac cgcgccttga aaagaggcca aagcctgcca tttttagctc
    33001 aattcggcga tgacctggat caatcgtccc ggcctgcttt tatcaactgc ataatcaatc
    33061 aaaattaccg aaatttcatg cataatcaca taaatcactt ttgcttatct tgtgtcagat
    33121 ttttttatct ccttatggat tttaggcaaa aacagtagca tgaaacgtca ttaccaatta
    33181 aggcagtata aaatgctggt tttctcgtca gttcaaggca ggataagggt taacacgcct
    33241 ttatgacagt caggaattga ctgtttctct aacgactctc cttttagcct taaagataaa
    33301 atctatttta atttcagcta tttaataaag aattttgcgc taaagcacat ttctgtacca
    33361 ataagcttgc catttgacct gtatcagctt tcccgataag ttggaaatcc gctggaagct
    33421 ttctggatga gcagcctgct catcatattt atgcagtaat tgagatcccc tcttcaccgt
    33481 attaaccgat gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa
    33541 accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca
    33601 gagcctaggg gaggttcacg atatgttgta cgataaatcc cttgagaggg ataactgtgg
    33661 tttcggcctg atcgcccaca tagaaggcga acctagccac aaggtagtgc gtactgcaat
    33721 acacgcactg gcccgcatgc agcaccgtgg cgcgattctc gccgatggta aaaccggcga
    33781 cggttgcggc ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg
    33841 cggctggcgt ttagcaaaaa actacgctgt cgggatgctc ttcctgaata aagatcctga
    33901 actcgccgct gccgcacgcc gcatcgttga agaagaactg caacgcgaaa ccttgtcgat
    33961 tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc
    34021 tctgccacgc attgagcaaa tttttgtgaa cgccccggca ggctggcgtc cacgcgatat
    34081 ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga
    34141 cttctacgtc tgtagcctgt cgaatctggt gaacatctat aaaggtctgt gtatgccggc
    34201 ggatctgccg cgcttttatc tggatcttgc ggacctgcgt ctggaatcgg ccatttgcct
    34261 gttccaccag cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc aaccgttccg
    34321 ctatctggcg cataacggtg aaatcaacac catcaccggt aaccgccaat gggcgcgtgc
    34381 gcgtacctat aaattccaga cgccgctgat ccctgacctg cacgacgccg cgccattcgt
    34441 caacgaaacc ggttctgact ccagttccat ggataacatg ctggaactgc tgctggcagg
    34501 cgggatggat atcatccgcg ccatgcgcct gttagtgcca cccgcctggc agaacaaccc
    34561 ggatatggac ccggaactgc gtgccttctt tgactttaac tccatgcata tggagccgtg
    34621 ggatggtccg gcgggcatcg tgatgtccga tggtcgcttc gctgcctgta acctcgaccg
    34681 taacggtctg cgtccggcac gctacgtcat taccaaagac aagctcatca cctgcgcctc
    34741 tgaagtcggt atctgggatt accagcctga cgaagtggtc gaaaaaggcc gcgtcggacc
    34801 aggcgaactg atggtgatcg acactcgcag tgggcgtatt ctgcactcgg cagaaaccga
    34861 tgacgatctg aaaagccgcc acccttataa agagtggatg gagaaaaacg tccgccgact
    34921 ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg acgacgacac
    34981 gcttgccagc taccagaaac agtttaacta cagcgcggaa gagctggact ccgtaattcg
    35041 cgtactgggc gaaaacggtc aggaagcggt cggttcgatg ggcgatgata ccccattcgc
    35101 cgtgctctcc agtcagccgc gcattattta cgactacttc cgccagcagt ttgcccaggt
    35161 gactaacccg ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat
    35221 cggtcgtgaa atgaacgtct tttgcgaagc agagggccag gcgcaccgtt taagctttaa
    35281 atcgccgatt ctgctctact ccgatttcaa acagctcacg acgatgaaag aggagcacta
    35341 ccgcgcagat acgctggata tgacctttga cgtcactaaa accacgctcg aagcgacagt
    35401 caaagagctg tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgt tgctggtgct
    35461 ctccgaccgg aatatcgcta aagatcgcct gccggttccg gctccgatgg cggttggcgc
    35521 gatccagacc cgtctggtcg atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac
    35581 cgccagcgcc cgcgatccgc accacttcgc cgtgttgctg ggtttcggcg cgacggctat
    35641 ttatccgtac cttgcctatg aaacgctggg ccgcctggta gacacccacg cgattgccaa
    35701 agattatcgt accgtgatgc tcaactaccg taacggcatc aacaaaggct tgtacaaaat
    35761 catgtccaaa atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc
    35821 ggtcggtcta cacgatgatg tagtgggcct gtgcttccag ggcgcggtca gccgcattgg
    35881 tggggcaagc tttgaagact tccagcagga tctgctgaat ctgtcgaaac gtgcctggct
    35941 ggcgcgcaag ccgattagcc agggcggcct gctgaaatac gtccacggcg gcgaatacca
    36001 cgcctacaac ccggacgtgg tgcgcacgct gcaacaggcg gtacaaagcg gcgagtacag
    36061 cgactatcag gaatacgcga agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct
    36121 gctggcaatt acgccgggtg aaaacgcggt caacattgct gatgttgaac cggcaagcga
    36181 actgtttaaa cgctttgata ccgccgcgat gtctatcggc gcgttaagcc cggaagccca
    36241 cgaggcgctg gcggaagcga tgaatagcat cggcggtaac tcgaactccg gtgaaggcgg
    36301 cgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc atcaagcagg tggcttctgg
    36361 tcgcttcggc gttactccgg cgtatctggt caatgccgac gtcattcaga ttaaagtcgc
    36421 ccagggcgcg aagccgggcg aaggcggtca gttgccgggc gacaaagtca ctccttacat
    36481 cgccaaactg cgctattcgg tgcccggagt gacgctgatc tccccgccgc cgcaccacga
    36541 tatctactct atcgaagact tagcgcagct tattttcgac ctcaagcagg ttaacccgaa
    36601 agcgatgatc tccgttaagc tggtgtccga accaggcgtt ggcaccatcg cgactggcgt
    36661 ggcgaaagct tatgcggact tgatcaccat cgcgggctat gacggcggca ccggcgcaag
    36721 cccgctgtcg tcagtgaaat atgcaggctg cccgtgggaa ctcggtctgg tggaaaccca
    36781 acaggcgctg gttgccaacg gcttgcgtca taaaattcgt ttgcaggttg atggcggcct
    36841 gaaaacgggt gtcgatatca tcaaagcggc gattctcggc gcagaaagct tcggcttcgg
    36901 cactggccca atggtggcgc tcggctgtaa atatctgcgt atttgccacc tgaacaactg
    36961 cgccacgggt gtagcaactc aggatgacaa actgcgtaag aatcactatc acggcctgcc
    37021 gttcaaggtg actaattact ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca
    37081 gctgggagta acgcgtctgg tggatctgat tggtcgcacc gacctgctga aagagctgga
    37141 cggtttcacc gccaaacagc agaaactggc gctgtcgaag ctgctggaga ctgccgagcc
    37201 acatgcaggt aagtcactct actgcactga aaacaacccg ccgtttgata acggcctact
    37261 gaacgcgcag ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt
    37321 ctggttcgat atccgcaaca ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc
    37381 ccagacgcac ggcgatcagg ggctggcagc cgatcctatc aaagcgtact tcaacggcac
    37441 cgcaggccag agtttcggtg tgtggaacgc gggcggcgtg gaactgtacc tgaccgggga
    37501 tgccaacgac tatgtcggta aaggcatggc gggcggctta atcgccattc gtcctccggt
    37561 tggttccgcc ttccgcagcc atgaagcaag cattatcggc aacacctgcc tgtatggcgc
    37621 gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc
    37681 cggtgctatc accgtggtag aaggcattgg cgacaacggc tgtgaatata tgacgggcgg
    37741 tattgtctgt attctgggta aaaccggcgt taacttcggc gcgggcatga ccggcggctt
    37801 cgcttacgtt ctcgatgaaa gcggtgattt ccgcaaacgc gttaacccgg aactagtcga
    37861 agtcttaagc gtcgacgatc tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga
    37921 gcatgttcag cataccggtt ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt
    37981 cgccacgaaa ttcgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca
    38041 ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag taaggggtag caacaatgag
    38101 tcagaatgtt tatcaattta tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa
    38161 gatccgtaaa attgagtttg ttgaaattta cgagccgttt tccgaaggcc aggccaaagc
    38221 gcaggctgac cgctgtctgt cgtgcggcaa cccatactgc gagtggaaat gcccggtaca
    38281 caactacatc ccaaactggc tgaagctcgc caacgagggg cgtatttttg aagcggcaga
    38341 actgtcgcat cagaccaaca cactgccgga agtatgcgga cgtgtctgcc cgcaagaccg
    38401 tctgtgcgaa ggttcctgca ccctgaacga tgagttcggt gcggtgacca tcggcaacat
    38461 tgagcgttat atcaatgata aagcgttcga aatgggctgg cgtccggata tgtccggcgt
    38521 gaagcaaacc ggtaaaaaag tggcgattat cggtgcaggc ccggcaggtc tggcgtgtgc
    38581 ggatgtcctg acgcgtaacg gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg
    38641 cgggctgctg accttcggta ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg
    38701 ccgtgaaatc ttcaccggca tgggtattga attcaaactc aataccgaag tgggccgcga
    38761 cgtacagctg gacgatctgc tgagtgatta cgatgccgtg ttccttggcg ttgggactta
    38821 tcagtcaatg cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc
    38881 gttccttatc gccaacacca aacagttaat gggctttggt gaaacccgcg acgaaccgtt
    38941 cgtcagcatg gaaggcaaac gcgtggtagt acttggcggt ggcgacactg cgatggactg
    39001 cgtgcgcacg tctgtacgcc agggggcaaa gcacgttacc tgtgcctatc gtcgtgatga
    39061 agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt
    39121 caaattcaac gtccagccac tggggattga agtgaacggt aacggcaaag tcagcggcgt
    39181 aaaaatggta cgtaccgaaa tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat
    39241 cgttgcaggt tccgaacata tcgttccggc agatgcggtg atcatggcgt ttggtttccg
    39301 tccacacaac atggaatggc tggcaaaaca cagcgtcgag ctggattcac aaggccgcat
    39361 catcgctccg gaaggcaacg acaacgcttt ccagaccagc aacccgaaaa tctttgctgg
    39421 cggcgatatc gtccgtggtt ccgatctggt cgttaccgcc atcgccgaag gtcgcaaagc
    39481 ggcagacggc attatgaact ggctggaagt ttaagctctg tcccagcgcc gcctcctcag
    39541 aaaagagtcg gcgctgaaat tctcctttct ttctggaaat cgcctcgcat aatctgaaga
    39601 aaaccaatgt aatacgctca tctggattgt taccggtaga tgcccggcgt atagctgatt
    39661 ccattattca acgcagcgcc atataaaggg acagataatg gaatcgctct cagagagaac
    39721 ctcaacaggc taccagcaaa tccacgacgg cattattcat ctggtcgata gcgcccgaac
    39781 ggaaacggta cgtagcgtta acgcgctgat gaccgcgacg tactgggaaa ttggccgacg
    39841 gattgtcgaa tttgaacaag gtggtgaggc cagagcagct tatggtgcac agttgattga
    39901 tcggctatcg aaggatttaa gtcgacggta taaacgtggg gtctctgcaa aaaacttacg
    39961 ccaaatgagg cttttttacc tcttttttca acatgttgaa attcgccaga cagtgtctgg
    40021 cgaattaaca ccattgggaa ttccgcagac accatctgca gaattcccat cggcaaagat
    40081 ttggcagacg ctgtctgcca aattgagagg ctgtttgata tttccacagc ttttgtattg
    40141 catctcctat tttcggtttg tatattcatg caaataaacg aagg
//
LOCUS       DADLSB010000019        39385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.019, whole genome shotgun sequence.
ACCESSION   DADLSB010000019 DADLSB010000000
VERSION     DADLSB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.019"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(71..1324)
                     /locus_tag="JN331_001001"
     CDS             complement(71..1324)
                     /locus_tag="JN331_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY7259205.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1557..2888
                     /gene="argA"
                     /locus_tag="JN331_001002"
     CDS             1557..2888
                     /gene="argA"
                     /locus_tag="JN331_001002"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY7259206.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2950..4776)
                     /gene="recD"
                     /locus_tag="JN331_001003"
     CDS             complement(2950..4776)
                     /gene="recD"
                     /locus_tag="JN331_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY7259207.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMFEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4776..8318)
                     /gene="recB"
                     /locus_tag="JN331_001004"
     CDS             complement(4776..8318)
                     /gene="recB"
                     /locus_tag="JN331_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY7259208.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="JN331_001005"
     CDS             complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="JN331_001005"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY7259209.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKDGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AIITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11375..14743)
                     /gene="recC"
                     /locus_tag="JN331_001006"
     CDS             complement(11375..14743)
                     /gene="recC"
                     /locus_tag="JN331_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY7259210.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKTLVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14756..15079)
                     /locus_tag="JN331_001007"
     CDS             complement(14756..15079)
                     /locus_tag="JN331_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY7259211.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15064..15471)
                     /locus_tag="JN331_001008"
     CDS             complement(15064..15471)
                     /locus_tag="JN331_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY7259212.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHCWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15468..16031)
                     /locus_tag="JN331_001009"
     CDS             complement(15468..16031)
                     /locus_tag="JN331_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7259213.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16022..16492)
                     /locus_tag="JN331_001010"
     CDS             complement(16022..16492)
                     /locus_tag="JN331_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7259214.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLYQQGETEGCL"
     gene            complement(16676..17470)
                     /gene="thyA"
                     /locus_tag="JN331_001011"
     CDS             complement(16676..17470)
                     /gene="thyA"
                     /locus_tag="JN331_001011"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY7259215.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17477..18352)
                     /gene="lgt"
                     /locus_tag="JN331_001012"
     CDS             complement(17477..18352)
                     /gene="lgt"
                     /locus_tag="JN331_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY7259216.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18503..20749)
                     /gene="ptsP"
                     /locus_tag="JN331_001013"
     CDS             complement(18503..20749)
                     /gene="ptsP"
                     /locus_tag="JN331_001013"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7259217.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20762..21292)
                     /gene="rppH"
                     /locus_tag="JN331_001014"
     CDS             complement(20762..21292)
                     /gene="rppH"
                     /locus_tag="JN331_001014"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY7259218.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            21977..22666
                     /gene="mutH"
                     /locus_tag="JN331_001015"
     CDS             21977..22666
                     /gene="mutH"
                     /locus_tag="JN331_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY7259219.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22735..23448
                     /locus_tag="JN331_001016"
     CDS             22735..23448
                     /locus_tag="JN331_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7259220.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23586..23804
                     /gene="ygdR"
                     /locus_tag="JN331_001017"
     CDS             23586..23804
                     /gene="ygdR"
                     /locus_tag="JN331_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY7259221.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23912..24952
                     /locus_tag="JN331_001018"
     CDS             23912..24952
                     /locus_tag="JN331_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY7259222.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24985..26178)
                     /gene="lplT"
                     /locus_tag="JN331_001019"
     CDS             complement(24985..26178)
                     /gene="lplT"
                     /locus_tag="JN331_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY7259223.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26171..28330)
                     /gene="aas"
                     /locus_tag="JN331_001020"
     CDS             complement(26171..28330)
                     /gene="aas"
                     /locus_tag="JN331_001020"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY7259224.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSCQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28915..29946
                     /gene="galR"
                     /locus_tag="JN331_001021"
     CDS             28915..29946
                     /gene="galR"
                     /locus_tag="JN331_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY7259225.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29953..31215)
                     /gene="lysA"
                     /locus_tag="JN331_001022"
     CDS             complement(29953..31215)
                     /gene="lysA"
                     /locus_tag="JN331_001022"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY7259226.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31337..32272
                     /gene="lysR"
                     /locus_tag="JN331_001023"
     CDS             31337..32272
                     /gene="lysR"
                     /locus_tag="JN331_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY7259227.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32259..32951)
                     /gene="ygeA"
                     /locus_tag="JN331_001024"
     CDS             complement(32259..32951)
                     /gene="ygeA"
                     /locus_tag="JN331_001024"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY7259228.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33080..34498)
                     /locus_tag="JN331_001025"
     CDS             complement(33080..34498)
                     /locus_tag="JN331_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7259229.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34790..35551)
                     /gene="kduD"
                     /locus_tag="JN331_001026"
     CDS             complement(34790..35551)
                     /gene="kduD"
                     /locus_tag="JN331_001026"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY7259230.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35581..36417)
                     /gene="kduI"
                     /locus_tag="JN331_001027"
     CDS             complement(35581..36417)
                     /gene="kduI"
                     /locus_tag="JN331_001027"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY7259231.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(36704..37878)
                     /locus_tag="JN331_001028"
                     /pseudo
     CDS             complement(36704..37878)
                     /locus_tag="JN331_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
     gene            38133..39362
                     /locus_tag="JN331_001029"
     CDS             38133..39362
                     /locus_tag="JN331_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7259232.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLCIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPSSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNHA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         9705 a        10703 c         9627 g         9350 t
ORIGIN      
        1 attgaacacc ctaacgggtg tttttttgtt tctgatctcc cataaaaaag cgccattcag
       61 cgccttttta tcatcccctt ctcgccagcg ttgccccatc ggcaaaatac gctttaatcc
      121 ccgcaagaat agactccgca acttcctgct ggaaagtcgc cgttttcagt ttacgctctt
      181 cctcaacgtt actgataaac gccgtttcga ccagaatgga gggaatatct ggtgccttta
      241 gtacggcaaa cccggcctgt tcaacttgat ttttatgcag cttgttgatt tttccgagct
      301 tattcagtac cgctttacca aacttaaggc tgtcagcaat agtcagcgac tgcaccatat
      361 cgaacatggt gtggtcgaca tagcggtcac cgcttttgct cacgccacca atcaagtccg
      421 aggcgttctg ggtttgtgcc agatattttg ccgcagtact ggttgcgcct ttggttgaga
      481 gcgcaaacac cgaggaaccg ctcggctgac gactggtaaa ggcgtcggca tggatagaga
      541 caaacaggtc agcacgctgt ttctgggctt ttgctacgcg cacttgcaac ggaatgaaga
      601 tgtcttcatt gcgcgtcatg tacaccttca tattgccctc tttctcgatc agagagcgca
      661 aacggcgagc tatttgcaat accacatctt tttcgcgcgt tttgtatttc cccaccgcac
      721 cggagtcttc gccaccgtgg ccagggtcaa gcataatgac aatcggacga tcgcgccctg
      781 ctttacccgg ttgtggacca ctttgcgctg gcggcacctg cttttcgagg tcgcctttgt
      841 tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg gccggataga
      901 ggtccatcac cagacgctct ttaaacccgg cgaccggcgc aagggcaaac agctgcggtt
      961 ttacgttttg ctttaattca aaaaccatgc gtacggtttg cgggtcaaat tgcccgacgc
     1021 gcgccgactt gatgaacggg tcgtctgcac gaatttgtgc cgccatcccc ttgagtaccg
     1081 agttcaggtt tacatcttcg atatccacca ccacgcgttc agggttactc aacgcgaact
     1141 gcttatattt cagctgacga tttgattcta ccgtcacgcg ggtgtagctg gacgcaggcc
     1201 agacgcgcac cgccacgacc tggctaaccg cagccaggct gacctgactt acgctcaata
     1261 gccacatagc gcccgcgcct tgcagtaaac gacgacggct gattgcagtg ttggatcctg
     1321 acatgcctct cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga ctaatttgac
     1381 cggaaacttt agcgaaagac gcataatctg tcatctaata aacggtaaac attcttttta
     1441 tattcacggc attactgata aaaaagtcgc tctcgcataa aatttacact tgcaccctgc
     1501 gaaaaaacag aataaaaata caataatttc gaataatcat gcaaaggggg tgtgccgtgg
     1561 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
     1621 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     1681 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
     1741 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     1801 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
     1861 gaacattgca actggatatt actgctcgcc tgtcgatgag tctcaataac acgccgctgc
     1921 agggcgcgca tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg
     1981 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc
     2041 atcgtcaact ggaaagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     2101 gcgagagctt taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag
     2161 ctgaaaagat gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg
     2221 tctccgaact tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg
     2281 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
     2341 tgcgccgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     2401 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
     2461 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     2521 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     2581 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
     2641 aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc
     2701 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     2761 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
     2821 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     2881 tagggtaagc gatgaaaatc accggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
     2941 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     3001 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac gacgacgcgc
     3061 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
     3121 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     3181 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     3241 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
     3301 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
     3361 cggatgacga tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc
     3421 agccacgcca aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg
     3481 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc
     3541 aagagcttcc tcaaacatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
     3601 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     3661 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     3721 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt
     3781 caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat
     3841 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
     3901 cactcgcgca tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
     3961 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
     4021 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
     4081 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
     4141 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
     4201 ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg
     4261 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca
     4321 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
     4381 aacctcaatg gcatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
     4441 catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct
     4501 gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
     4561 gacacaggtc gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa
     4621 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
     4681 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
     4741 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
     4801 gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
     4861 tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga
     4921 agatcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa
     4981 agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg
     5041 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
     5101 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
     5161 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
     5221 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
     5281 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
     5341 cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
     5401 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
     5461 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
     5521 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
     5581 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
     5641 agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
     5701 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
     5761 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
     5821 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
     5881 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
     5941 tcgacctccg cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac
     6001 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
     6061 cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt
     6121 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
     6181 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc
     6241 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
     6301 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
     6361 cgccagattt gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca
     6421 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
     6481 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
     6541 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
     6601 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg
     6661 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
     6721 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
     6781 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
     6841 cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
     6901 atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
     6961 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcgtgaac ttcactacgc
     7021 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
     7081 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
     7141 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
     7201 gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
     7261 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
     7321 gccaatgcgc gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
     7381 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
     7441 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
     7501 gcccaggcgc tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga
     7561 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
     7621 tttaccgtat caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg
     7681 ggcggcggtg ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgcgcagc
     7741 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
     7801 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca
     7861 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
     7921 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
     7981 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg
     8041 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
     8101 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacgatcagc
     8161 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
     8221 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
     8281 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
     8341 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
     8401 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
     8461 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
     8521 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
     8581 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattatcg cctgctggat
     8641 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
     8701 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
     8761 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
     8821 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
     8881 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
     8941 gctgttaccg tctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
     9001 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
     9061 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
     9121 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
     9181 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
     9241 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
     9301 ctgctcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
     9361 cagacgctgg gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa
     9421 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
     9481 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
     9541 aatcaggctg acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac
     9601 gcgcagattc gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat
     9661 cagcgagaaa tcatcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc
     9721 ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc
     9781 atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc
     9841 attctgcggc gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc
     9901 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
     9961 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    10021 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    10081 aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcggtta aagacgcaga
    10141 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    10201 aactaatccc tgcttttgca gccagtcaga aagtgtacct gggctgcgat tgccaatcag
    10261 ataggtaatc aattcatcgg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    10321 ctcaacgcgc aacactttac gcggcagcgc agggacgtaa tgaatgataa tgcccttttg
    10381 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    10441 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    10501 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    10561 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    10621 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    10681 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    10741 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    10801 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    10861 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    10921 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    10981 gggcactacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    11041 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    11101 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aatggtgccc agagggcaac
    11161 aaacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    11221 ttattttctg gacagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    11281 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    11341 taagcggata gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg
    11401 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    11461 gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    11521 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    11581 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    11641 atggcgcgga cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    11701 gttcggctgc aagcggcgga aaacgccact caccgtcttt gcgtagaaaa aggcgacttt
    11761 cgccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    11821 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    11881 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    11941 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    12001 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    12061 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    12121 gtccttccag aataaatggc tcggtgtctg ggatttcgct gtcttcagta cggaagttca
    12181 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    12241 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    12301 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    12361 ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    12421 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    12481 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    12541 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    12601 ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    12661 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    12721 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga
    12781 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    12841 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    12901 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    12961 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    13021 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    13081 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    13141 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    13201 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    13261 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    13321 ccagcagcgc cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctga
    13381 taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca
    13441 ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat aaacggactg tagctgtcga
    13501 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg
    13561 ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaatg
    13621 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    13681 tgttcacacc cgcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    13741 gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag
    13801 aaaggagata aatgtagtcg cgcccaagct tcccccatga ggccagcagc gggttgccga
    13861 catcctgttc accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg
    13921 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag
    13981 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    14041 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    14101 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    14161 cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg ttgtccgagt tcatgggtat
    14221 attccaccag cgtcttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    14281 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    14341 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    14401 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    14461 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    14521 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    14581 caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    14641 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    14701 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    14761 gacgattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    14821 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    14881 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt
    14941 actggtttct gctggcgaga ctgttcatta atgttcgctg gatacccgat aacgcagtga
    15001 caatcatcac catcagcacc atcgcaacca ttacctccgg caggctaaag ccttgttgat
    15061 tcttcaggga agctgacata atgccccctc tttcagcgga caaaaatcgc tccagccgcg
    15121 tggcgaaaaa acaatttttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc
    15181 ataaccggca atcaataagg cttcattatc tgccagtaaa cgcaaacaaa cctgggcatc
    15241 ggttccagcg tactgcgagc actgaactgc tggctgcgtc tgccagcagt gcatttttcc
    15301 ccacgccagc gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac
    15361 gcgagaggca aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaac
    15421 cagcagcatc aggaccagag ccagtgacga aacacctttt tcgcggttca caggttgaat
    15481 cctgtcacgc tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc
    15541 atattaaccg tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg
    15601 tcgataatga tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc
    15661 gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcggactct
    15721 tttaccggtt cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag
    15781 tcaccctgtc cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc
    15841 ctctggagat gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagtgccagc
    15901 ttacgagtgc tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc
    15961 aacaatacgc tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct
    16021 tttacaggca tccttctgtt tctccttgct gataaagccg gagtcttccc cacggagaaa
    16081 ccaccagcca ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat
    16141 tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc
    16201 gtggcacaaa gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca
    16261 caaggcacca taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt
    16321 tggcatcttc acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccaaagcc
    16381 gttgcgactg ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa
    16441 tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag
    16501 tatggagcga ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg
    16561 actatttacg ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaac
    16621 ggagggtaaa aaaaccgacg cacactggcg tcggctctgg caggatgttt cgtaattaga
    16681 tagccaccgg cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga
    16741 aacggtagtc gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg
    16801 gttcgcggct taattgcaga tgagtttgat ccatatggtt gctgtacagg tgcgtatcgc
    16861 caccggtcca gacaaaatca cccacttcca gatcgcactg ctgcgccatc atatgcacca
    16921 gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa gacgtcacag gagcgctgat
    16981 aaagctggca agagagtttg ccgtctgcca catagaactg gaagaatgca tggcacggtg
    17041 ccagcgccat tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgcgaat
    17101 ccgggtcgtt tttcagctgg ttcagtaccg tagtaatctg gtcaatatga cgaccatctg
    17161 gcgttggcca ggcgcgccac tgtttaccat acactggccc gaggtcgcca ttttcatcgg
    17221 cccattcgtc ccagatggtg acattgtttt cgtgtagata agcaatgtta gtatcgccct
    17281 gcaggaacca cagcagttcg tggatgatgg aacgcaggtg gcaacgtttg gttgtcacca
    17341 gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg
    17401 ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat
    17461 actgtttcat ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca
    17521 tcatgatcac acccgcgaca atcatcggga tggaaagaat ttgccccatg ctgatgtact
    17581 gcacccaggc accggtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa
    17641 acgcgccgta gccaatcagg aacaagcctg agacagctcc cattgggcgc ggtttacgaa
    17701 tatacaggtt gaggataata aacagcacca caccttccag cagcagttcg taaagctgtg
    17761 atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc gggttggttt
    17821 gcagcagcaa aatatcttct gtacgggagc ctgggaacag catggcaaac gggaagttcg
    17881 ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc cagacgcccg gcaccaagac
    17941 caaacggaat gagtggtgcg ataaaatcag agacctggaa gaaggaacgt ttagtacggc
    18001 gggcgaagat aatcatcacc acgataacgc caatcaggcc gccgtggaaa gacatgccgc
    18061 cgtcccagac acggaacaga tacagcggat cggccataaa ctgcgggaaa ttgtagaaca
    18121 gaacataacc aatacgtccc ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt
    18181 caacttcatt tttggtccag ccactgcccg gacgattcgc ccgacgtgtt gccagccaca
    18241 ttgcaaaaat gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta
    18301 ttgagaaaat gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc
    18361 acaagttctt gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca
    18421 ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta
    18481 aaagatatgt atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccgcgac
    18541 gctccataaa ggctgcaacc tgatgacgaa cttcggtcgc cagttgcgct tccagactac
    18601 gctgcgcaag attttctgct tcggcaaaat caatgcgccg cagcaggtat tttacccgcg
    18661 ctacagaacg tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc
    18721 acatgggatc gcccgccatt tcaccgcaca aacggagatc gataccatgt atttccgctt
    18781 cccgggcgat catcgccaga gctcgtaaca ttgcaggatg aagactgtcg taaatgttcg
    18841 ccacccgggt attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa
    18901 cagagatgaa atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt
    18961 ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac
    19021 gtccggcacg ttcaatcagg cggcgtgctt cgtcaacttc atcgaggctg gtgaccatcg
    19081 gcaacagaat attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga
    19141 tcaagaagat ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc aggcatggat
    19201 tctcttcgct gatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca
    19261 aggtgacggg tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt
    19321 cttccgacgg gaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc
    19381 catcaatacg gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga
    19441 cttttatacg ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac
    19501 ggctaagctc aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg
    19561 gatcgaccag caattcaccg cgatagccat caacgatcag cgtcctacga tgcagcaccg
    19621 aaggctgaat atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag
    19681 catgggagtt ggctgcgcca tctcgcacga caacaccgac taagcgatcc tggggcagct
    19741 cagcaagagt tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt
    19801 tcggcccttg attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat
    19861 cgccagcccg ctctttgaga tagttatcgc ttagcgcggc aaactgttca gcaaattttt
    19921 caatgaccgt ttttaccgcc cactctgcca ccgagccttt atcaacctcg gcaaacaatt
    19981 cgcgacgcag ccgggtgtcc gaaagcaggt gcgagtaaag atcgaaaata gccgccgttt
    20041 ctttttgtgc tccggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt
    20101 ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca
    20161 cctgttccat taaaggtaac gtggcatcct gccagccttc ggcaatcgcc acaccaggtg
    20221 ctgccggtaa tgcgcggatt cgcgtctggc gatattgccc aaacagggca gtcaactgcg
    20281 actgagaaag aatagctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt
    20341 cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt
    20401 gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttacggag gggatgtatt
    20461 tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca
    20521 ggccgacgat cccttcatca aacgcgagcg ttacagtgcg accgcgtggt tttttcagcc
    20581 cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga
    20641 cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc
    20701 gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga
    20761 cttaacctct tttacgtcga taagcagatg cgttttgtgg ttttggcgta ttttcctgca
    20821 gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg
    20881 acaccacctg tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg
    20941 tactgctggt ttgcatattg atttctgcat cgccgcttac cagctgcaag agaaaccatt
    21001 tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt
    21061 tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta
    21121 cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc
    21181 cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc
    21241 gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac
    21301 ctcaaaataa agctttatat acgaatgatt gtttcatact ccaggaagac ggtaaaccac
    21361 tctctgcagg gcattacaca ctaataacaa ttgaataact cacagttatg tgcagagtta
    21421 taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga
    21481 agaactacgg aattactacg ggaaaacccg gagcattctg aataagcccg atatagacat
    21541 tttaaaatat tcattattag tcaatgcatt acatgtaatt tgagtaatga aaaagagtaa
    21601 ttcgtgaccc aggtcacacc tctcatttac gggttggcga aagatcaacc aatgccgtat
    21661 ttatccacag aatgagccac taagttaagc actgaaccac taaaaactgg agtttcgtcg
    21721 cacgtcaagg ctgtaaatga aaacagtagt ggaggttttt cacagttatc ccagctttct
    21781 gtggataaca tggtgtaaga tcctgtttat tttcagtgac cagatttgga aaacctattg
    21841 cagtgttgcg caactcgatt accggcagcc taaaaagcaa tataaatcag agaattgaac
    21901 aacacatgtg gaaaaagtta cactgcgaat attcggcaca taattgctgt ttgtttttta
    21961 accaaggtat catgacatgt cccaacctcg cccactgctc tctcctcccg aaactgaaga
    22021 acagttgtta gcgcaagcac agcaactttc tggttataca ttgggagaac tggcggcact
    22081 tgccgggctg gttacgccag agaatttaaa acgcgataag ggctggattg gcgtgttact
    22141 ggagatctgg ctaggtgcca gcgcagggag taaacctgag caagattttg ctgctctggg
    22201 cgtggaactt aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa caacattcgt
    22261 ttgtgttgcc ccgttaacgg gtaatagcgg ggtgacctgg gaaaccagcc atgtgcgcca
    22321 caagctcaaa cgcgtactgt ggataccggt tgaaggcgag cgcagcatcc cgctggcgca
    22381 gcgtcgcgta ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc gacagctacg
    22441 cgaagactgg gaagaattaa tggatatgat tgttctcggt caggttgagc ggatcaccgc
    22501 tcgtcacggg gaatatttac agataagacc gaaagcagcg aatgcgaaag cgcttaccga
    22561 agccattggt gcccggggcg aacggattct gacgctgcca cgcggctttt atttgaagaa
    22621 gaatttcacc agtgcactac tggcccgtca ttttctgatc cagtagccat cgctttgacc
    22681 tgccgctttc cgggcatata attaccgctt catttttttg gcagggcttt ttagatgtta
    22741 tttgcatgga taaccgatcc taacgcctgg cttgcgctcg gtacgctgac gctgctggag
    22801 atcgttcttg ggatcgacaa tattattttc ctttctctgg tggtggcaaa gcttcccaca
    22861 gcacaacgtg ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat gcgtctggcg
    22921 ctgctggcat caatctcttg ggttacgcgc ctgacaaatc cgctttttac aatattcagt
    22981 caggaaattt ctgcccgtga tttgattctg cttctgggtg gcttgttcct tatctggaaa
    23041 gccagcaagg aaatccacga atccattgaa ggtgaagaag aagggctgaa aacacgcgtt
    23101 tcatcattcc tcggcgctat cgtgcagatt atgctgctgg atattatctt cagcctcgac
    23161 tcggtgatta ccgctgtggg tctgtcagat cacctgttta ttatgatggc agccgtggtg
    23221 attgccgtag gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt cgaacgccat
    23281 ccttcggtaa aaatgctggc gctctctttc ctgattctgg tgggctttac cctgattctg
    23341 gaaagtttcg acatccacgt accgaaaggt tacatctact tcgcgatgtt cttctctatt
    23401 gcggttgaaa gcctcaactt gattcgcaac aaaaagaatc cgctctgata ctccgtacgc
    23461 tctcctgcag gagggcgtac tcactccttt taccctcaca gtttaagatt ttcctgcttt
    23521 caagatatat aacgtcggtt tataaacaga ctattatcat aggtaggcca aatgaggata
    23581 accggatgaa aaaatgggca gtaataattt ctgcagtcgg actggcgttt gctgtttccg
    23641 ggtgttccag tgattacgtc atggcgacca aagatggccg tatgattttg accgatggaa
    23701 aacctgaaat tgatgatgat accgggctgg tgagttatca cgatcagcaa ggtaacgcga
    23761 tgcaaattaa ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata aggtcagcat
    23821 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
    23881 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
    23941 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
    24001 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
    24061 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
    24121 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
    24181 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
    24241 ccgcgaagcg ctgcatgaca gcctcaagcg cctacagact gattacctcg atctttatca
    24301 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
    24361 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
    24421 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
    24481 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
    24541 gttaaaccgc agttttgaag taggtctggc agaagtcagc cagtatgaag gggtcgaact
    24601 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
    24661 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
    24721 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggccttg atcctgctca
    24781 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
    24841 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
    24901 attagctgaa attgaagcgg tacatcaggt ttatacttat cctgcaccat aaagattaac
    24961 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
    25021 tgccagcgca aatagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
    25081 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
    25141 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
    25201 gagcggaacg acaaaaaagc cccccagcac gccaatcagc atcaacaagg cataggctgg
    25261 cagcagctcg tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa tcccggctgg
    25321 catacagcgt gacacggttt ccagcgtaac cagcttcgct gccgcacctg cgccaaccac
    25381 gataccaatc gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatgcccag
    25441 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
    25501 actggtgccc accagcgaaa aacgcgtttc accattgcac catagcgagg tgcaggcatt
    25561 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
    25621 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
    25681 ggcgaccagg acatgccagt cagccagcac accaccggct acggaaccga gtaaaatcgc
    25741 cgctatggta gaagcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa
    25801 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    25861 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    25921 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    25981 cgccaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    26041 atagaactgc gctttcagta acgccagagt ggcaaacagt agcgcgttat cgccaaacgc
    26101 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
    26161 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
    26221 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
    26281 ctgcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
    26341 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
    26401 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    26461 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
    26521 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
    26581 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    26641 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
    26701 ggcgtgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    26761 cgttgataga aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc
    26821 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
    26881 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    26941 tcgaggtacc gaacaacacg gtgcaactgc ggtcatacac caagtccggc acaatgcggt
    27001 aatgcagcgg gcttggataa aggaacactt ctgcacctgt aagcagtggc gtaaacaggc
    27061 ctaccgtcag cccaaaggag tgaaacagcg gtaatgccga cataaagcga tcgttggtgg
    27121 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
    27181 cgacgccttt tggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
    27241 gctgctgttt tacctgcgcc aggcgcggca tcagcaaatg agcgaagatc catactttgt
    27301 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    27361 gctccggcag atgccagagt ttgcctttat cgagaaactg gcaggaagtg aagatggttt
    27421 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
    27481 ttgcgggaat gcggcgacgc gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    27541 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    27601 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
    27661 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagagttt
    27721 cacgcgggcg caccgccata cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    27781 ttttgcgacg gtcacgggca cgcggcgcat ccggcatcgc cacctgcgtt ggaggcaaaa
    27841 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    27901 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    27961 caaaacccgc gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag
    28021 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag
    28081 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    28141 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
    28201 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
    28261 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    28321 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    28381 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    28441 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    28501 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
    28561 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
    28621 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
    28681 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
    28741 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc ttttttgtct
    28801 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccacccct tgaaccaacg
    28861 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
    28921 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
    28981 aataattcac ccaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct
    29041 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
    29101 ggtctgatcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
    29161 caggtggctt atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa
    29221 aaagagcgtc aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
    29281 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
    29341 ctgatcaacc gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac
    29401 ggtgcctggc tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg
    29461 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
    29521 gctgaaagtg gtattccggc caatgatcgg ctggtgacat ttggcgaacc agacgaaagc
    29581 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
    29641 tgttataacg attcaatggc ggcgggcgcg atgggcgttc tcaatgataa tggtattgat
    29701 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
    29761 ccgcgcctga ccaccgtgcg ttacccaatc gtaacgatgg cgacccaggc tgccgaactg
    29821 gctttggcac tggcggataa tcgccctctc ccggaaatta ctaatgtatt tagtccgacg
    29881 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
    29941 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
    30001 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctattgta
    30061 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc
    30121 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
    30181 gcccgattca cataacggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
    30241 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
    30301 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
    30361 ccgcacctga gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc
    30421 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
    30481 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
    30541 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc
    30601 acacacctgc tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
    30661 tagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
    30721 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
    30781 cggattaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
    30841 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
    30901 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
    30961 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
    31021 cattaagcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacttcaaa
    31081 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
    31141 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
    31201 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
    31261 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga
    31321 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
    31381 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
    31441 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg
    31501 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
    31561 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
    31621 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
    31681 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
    31741 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
    31801 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
    31861 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaattacat
    31921 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
    31981 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
    32041 ggcgggggta ggtgtttcgg tggttaaccc gctcaccgca ctagattatg cggcaagcgg
    32101 tttagtggtg cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
    32161 gcaccgcccg tcatcagcgc tggttcaggc gtttagtggg catttacaag cggggttacc
    32221 gaaactagtc acttctcttg acgctatttt gtcgtcagct acgacagcat aaaagcgaca
    32281 gcatcctcgg catggatcgc cgcggtatca aacacaggca aaacactgcg ctcttctggc
    32341 accagtaaac caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg
    32401 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
    32461 tcttcaaaaa taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg
    32521 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
    32581 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
    32641 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
    32701 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
    32761 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
    32821 tcgacgctat gtagcagcat ttgcgcagag tgaagcccac caagccgctg tttaatgcct
    32881 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
    32941 attgttttca ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag
    33001 acaaacgcct caacggccag gtgcccaatc tctattaacg aaaaaagggc cggatgtaca
    33061 gcacatccgg cccgtgaaat cagacgccga tatttctcaa cttctcgcct gccatcagtt
    33121 tgcgttcgat atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtaatgc
    33181 ccacaaacgc aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctat
    33241 caagcagtgt caggaaggtc gcgccgataa tcatattcga cacccagttt gtcgtcgtcg
    33301 aacaggtaat accgaaatcg cggcatttca gcggctgaat ttcagagcac aggatccaca
    33361 ccactggcgc ggcgctcatc gcataaccgg caatacacat catcgtcatg ccaacagaga
    33421 gccaagacaa gccactggaa gccgtaccgt tatcaaactg catcaggcaa tagcccagca
    33481 ccagagtgcc taacgccatc acgctgaaac caattttcag agccggttta cgacctgctt
    33541 tatctaccgt aaagaccgca atgaaggtcg cgaacataaa ggtcagtccc acgaccagag
    33601 tcgcaatcat ctgttgttct gtggtcgtaa agcccgccat tttgaagata cgcggcgcgt
    33661 agtacatgat gatgttcata ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa
    33721 acacagcacg acggacgtta cggttgatct taaacagtgc ccaaccgccc tgttttaact
    33781 tcaggctttc acgaatttcg ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca
    33841 tacgcaacac ttcttccgcc tcaatatgac gccccttttc cgccagccag cgcgggctat
    33901 ttggcaggaa gaccaccaga ataatcagca gaactgctgg taaagcaaga acccccaaca
    33961 ttgcgcgcca gttaccgcta taactgaacg ctgtatcgga taaaaacgcc agcacgatgc
    34021 cgagtgtgac catcaactgg tacatactga tcatcttacc gcgaacgttt tcacttgcca
    34081 tttcagaaag atacagagga gcggtgtaag acgcgatccc gacagcaatg cccagcacca
    34141 cacgagcggc gattaacatc tctacgctgg tcgcaaaagc ggaccctata gaaccgagta
    34201 caaacaggat ggcccccgcc atcaggctgt atttacgccc caggcggaac gacagccaac
    34261 cattaaacag cgcaccaatt gctgcgccga gcatcatgct actaaccacc cattcctgca
    34321 aacgactggt cagcacaaag tgatcggtaa tgaacggcaa cgctccggcg attacgccga
    34381 tatcaagacc aaataacaat cctgcgaccg cagcagctac cgaaacaaac atattcatac
    34441 gccgcgtatc acgcaaagaa cgtggcgtta aagcagattc cgtattgata gtaaccattt
    34501 tttcctgcca gcagagagta agacatagtg aaaaaatacg tgaacaattc acacaggtgt
    34561 caggtcggaa acagcataaa tatggattaa attgctgcga catgccgtta tgtgatggat
    34621 attccaattt taaaattaag ttgaattatt gagattatta ttaaccacct aattttaaag
    34681 cagataaaat tcataaagtt cattaattga taattgatat ggattatgat gatctagaga
    34741 tataaaaaac cctgccatgc ggcagggtca taaaagtaag aggaatgaat taacgcgcca
    34801 gccaaccgcc atccacagca atggtataac catttacata atctgaagcg ctggaggcaa
    34861 gaaacactac cggccccatc aggtcactcg gcagtcccca acggccagct ggaatgcgat
    34921 cgagaatttc cgcgctacgt tgttcatctg cccgcagttg ttgggtattg ttggtcgcca
    34981 tgtaacccgg agctatcgca ttaacattaa tgttgtgttt agcccattcg tttgccatca
    35041 atcgcgtcac acccatcacg ccgctttttg atgcggtata agaaggcaca cggatcccgc
    35101 cctggaagga gagcattgac gcgatattga taatcttgcc gccattgcct tgcgcgataa
    35161 agtgtttcgc cgctgcctga gacatgaaga atacgctctt gatattcagg ttcatgacat
    35221 cgtcccagtc tttttcgctg aactcgagag cgtcttcgcg gcgaatcaat ccggcgttat
    35281 tcaccaggat atcaatatga ccaaattcag ctaccgcgcg atccagcagt ccaggaatgc
    35341 catcaatctt tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct
    35401 gctcgatggt ttcagtcggt tcaacgatgt taatgccaac aatgtcacag cccgcttgcg
    35461 ccagccccaa cgccatcccc tggcccagcc cagtatcaca accagtgacg accgcaactt
    35521 taccttggag agaaaatgca cttaaaatca taacaacacc ttattcgttt atgcccacaa
    35581 ctagcgcaaa tctttaacgg ccacatggtc catatcatta aagacctggt tttcaccgac
    35641 catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctcgg
    35701 ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat
    35761 catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg
    35821 ctcgtgggtg tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc
    35881 catactcaat tgacaggttt ccagtacatc cgggacaaaa tatttgttaa tcgtgcgacg
    35941 gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt
    36001 ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aacttcgccg gagtggcggt
    36061 atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg
    36121 accgatttca tagcattgac catcgacagt aatcgtaccg gcaccgccaa tattgataac
    36181 acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc
    36241 aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct
    36301 gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag
    36361 cccttgggta tccagcgttt ttgcgtgcgc accgtggatg ctctgtctta cgtccacatc
    36421 aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt
    36481 tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt
    36541 ttcattttta agttgaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa
    36601 tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag
    36661 attaatgacg gcagatattt atctgtaagg aggaaatgca gagctactcg tcacgttcaa
    36721 tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat
    36781 tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg
    36841 ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca
    36901 tcttgccaac cgaaagcgcc tgtgcagcaa ccgcttcatt agcctcgata agatcgacat
    36961 cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc
    37021 ccatcaatgc ccaccaacgc tggcaaatgc gcggatgcgg gccagcacgg gtaaattcaa
    37081 cgctcgagct ttggcttcgc tcatcatcat taccgcagct gcgccatcgt ttatggatga
    37141 tgcattcccc gcggtcacag aaccgagact atcaaatgaa ggatttaaac gggctaaacc
    37201 ttctgcactg gcgtcagtgc gtggctgttc atcggtatca acaaccaacg tctgaccgtt
    37261 actttggctc attaccggga cgatctcatc tttaaatcgt ccggcgtcaa tcgccgctcg
    37321 cgctttttgt tgcgagctaa gtgcgtaagc atcctgcaac tgacggctga tgccatattc
    37381 gcgagccaga ttttcggcgg tgacaccaat atgataatca ttgaaggcat cccacaaccc
    37441 atcatgcacc agactgtcaa ccaactggct attgccaagc tgtgcaccgg tgcggctatc
    37501 agtcagaaca tgtggtgcgc ggctcatgtt ttcctggcca ccggcgatga caatatcagc
    37561 ctcgccacac tgtatcgcct gagtagccag atgcagtgct ttaagaccgg aaccgcaaac
    37621 gtcattaatc gtgattgcag aaacgctgtt aggaagacca cctttaatag ccgattgcct
    37681 tgccggattc tgccctgccc ctgcagtcaa cacctgacca agaattactt catccaccgc
    37741 atatgcagga acgccggtac gttctattaa cgctttcacg accagactac caagttccac
    37801 ggcagaatga cccgctaacg caccacgaaa gcagccgata ggtgtccgta acgccccgac
    37861 aatcacaacg tctttcatca caacctcgca ccaattaaca gtgcaatagt aaatgattgt
    37921 taattacagt tatcttaatt gtttaaaaaa agtgattttt atcacaaagg aaatatgcct
    37981 gagcagtagt cagagtcata actggcacgt aaggtttgca accactaacc caccaataga
    38041 tgggtagata gggcgttaat ctcccatact taacttggtt tatggtaaat tgctctccat
    38101 tttgtttaat ttgtagatga tacgttcaga taatgtctaa tatttggtct aaagaagaaa
    38161 ctctgtggag tttcgcgctc tacggcacag ccgttggtgc aggcacgcta ttccttccta
    38221 ttcagttagg ttcggcaggg gctgtggtcc tgtttattac tgctctggtc gcctggcctt
    38281 taacatattg gccacataaa gccttatgcc agttcatcct ctcatcaaaa acatcagcag
    38341 gtgaagggat aacgggcgcg gtaacacact actatggcaa gaagattggt aatctgatta
    38401 ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat atatgcagtg gcaattacca
    38461 actcacttac ggaacagctg gcaaagcata tggttattga tctttgcatc cgtatgttgg
    38521 tgagtctggg tgttgtatta attctgaatc tcatttttct gatgggacgt catgccacta
    38581 ttcgggtaat gggatttttg gtattcccat tgattgccta tttcttattt ctttccattt
    38641 accttgtcgg tagttggcaa cctgatctat taacaaccca ggtagagttc aatcagaata
    38701 cccttcacca gatatggata tcgattcccg tgatggtttt cgcctttagc catacgccca
    38761 ttatttctac gtttgccata gacagacgtg aaaaatatgg cgaacacgct atggataaat
    38821 gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat aagtgtactg ttctttgtct
    38881 ttagctgcct gctttctatt ccatcttcgt atattgaagc ggctaaagaa gaaggggtca
    38941 ccattttatc ggcgctttct atgctgccga acgccccagc atggttgtca atttccggga
    39001 ttattgtcgc agtagttgcg atgtcgaaat cattcctagg tacgtacttt ggcgttattg
    39061 aaggtgccac agaggtcgtc aaaacaacac tacagcaggt tggtgtaaag aaaagtcgtg
    39121 catttaacca cgcactatca attatgttgg tatcgctgat taccttcatt gtttgttgca
    39181 ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg cccgctcatt gccatgatac
    39241 ttttcatcat gcctacgctg tcaacgtatc tcatcccggc gcttaaaccc tggcgttcca
    39301 tcggaaatct gattacgctg atcgtgggta tcctgtgcgt atcggtaatg ttctttagct
    39361 aatatccgtt gtggatggtt caaaa
//
LOCUS       DADLSB010000020        38023 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.020, whole genome shotgun sequence.
ACCESSION   DADLSB010000020 DADLSB010000000
VERSION     DADLSB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.020"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(21..1019)
                     /gene="fbp"
                     /locus_tag="JN331_001030"
     CDS             complement(21..1019)
                     /gene="fbp"
                     /locus_tag="JN331_001030"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY7259233.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1195..2568
                     /gene="mpl"
                     /locus_tag="JN331_001031"
     CDS             1195..2568
                     /gene="mpl"
                     /locus_tag="JN331_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY7259234.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2651..3202)
                     /locus_tag="JN331_001032"
     CDS             complement(2651..3202)
                     /locus_tag="JN331_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY7259235.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3296..4648
                     /gene="pmbA"
                     /locus_tag="JN331_001033"
     CDS             3296..4648
                     /gene="pmbA"
                     /locus_tag="JN331_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY7259236.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKVPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4831..5217
                     /gene="cybC"
                     /locus_tag="JN331_001034"
     CDS             4831..5217
                     /gene="cybC"
                     /locus_tag="JN331_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY7259237.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLADNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5262..5726)
                     /gene="nrdG"
                     /locus_tag="JN331_001035"
     CDS             complement(5262..5726)
                     /gene="nrdG"
                     /locus_tag="JN331_001035"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY7259238.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5884..8022)
                     /gene="nrdD"
                     /locus_tag="JN331_001036"
     CDS             complement(5884..8022)
                     /gene="nrdD"
                     /locus_tag="JN331_001036"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY7259239.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFAGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8416..10071)
                     /gene="treC"
                     /locus_tag="JN331_001037"
     CDS             complement(8416..10071)
                     /gene="treC"
                     /locus_tag="JN331_001037"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY7259240.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPCFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNGAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10121..11542)
                     /gene="treB"
                     /locus_tag="JN331_001038"
     CDS             complement(10121..11542)
                     /gene="treB"
                     /locus_tag="JN331_001038"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAY7259241.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11661..12608)
                     /gene="treR"
                     /locus_tag="JN331_001039"
     CDS             complement(11661..12608)
                     /gene="treR"
                     /locus_tag="JN331_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY7259242.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12793..12846
                     /gene="mgtL"
                     /locus_tag="JN331_001040"
     CDS             12793..12846
                     /gene="mgtL"
                     /locus_tag="JN331_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY7259243.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12987..15683
                     /gene="mgtA"
                     /locus_tag="JN331_001041"
     CDS             12987..15683
                     /gene="mgtA"
                     /locus_tag="JN331_001041"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY7259244.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15889..16275)
                     /gene="ridA"
                     /locus_tag="JN331_001042"
     CDS             complement(15889..16275)
                     /gene="ridA"
                     /locus_tag="JN331_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY7259245.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16348..16809)
                     /gene="pyrI"
                     /locus_tag="JN331_001043"
     CDS             complement(16348..16809)
                     /gene="pyrI"
                     /locus_tag="JN331_001043"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY7259246.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16822..17757)
                     /gene="pyrB"
                     /locus_tag="JN331_001044"
     CDS             complement(16822..17757)
                     /gene="pyrB"
                     /locus_tag="JN331_001044"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY7259247.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17761..17895)
                     /gene="pyrL"
                     /locus_tag="JN331_001045"
     CDS             complement(17761..17895)
                     /gene="pyrL"
                     /locus_tag="JN331_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY7259248.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18176..18571)
                     /locus_tag="JN331_001046"
     CDS             complement(18176..18571)
                     /locus_tag="JN331_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7259249.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18703..19416)
                     /gene="bdcA"
                     /locus_tag="JN331_001047"
     CDS             complement(18703..19416)
                     /gene="bdcA"
                     /locus_tag="JN331_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7259250.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGTLDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPIRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19487..20080
                     /locus_tag="JN331_001048"
     CDS             19487..20080
                     /locus_tag="JN331_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7259251.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20225..20677
                     /locus_tag="JN331_001049"
     CDS             20225..20677
                     /locus_tag="JN331_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7259252.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20800..21617
                     /locus_tag="JN331_001050"
                     /pseudo
     CDS             20800..21617
                     /locus_tag="JN331_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21732..22517
                     /locus_tag="JN331_001051"
     CDS             21732..22517
                     /locus_tag="JN331_001051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259253.1"
                     /translation="MINLSHILIDSLDDAKVNLTPVIHSMLVTFLELPYNNDVKILEW
                     CFNKSMQYFDDSAKIEHACSVINHINFLCDQSKVAETLFFNLDKEPYKNSPELQELIW
                     KKLVVYVNDFNLSNREKTYLIQRIFNNVESLFNKVPVSILVNDILLNDFFMKNTEMIN
                     WYFPRLLKSYEGEKNYFDNLKYDINDEKSNKEILKNQPDNVIKEKLNNEFKLRFRMMQ
                     TIFKSKVNVSPYIDQQRLNTLNPPENLRMAIEKFGWKKKTITA"
     gene            complement(22570..23583)
                     /gene="argF"
                     /locus_tag="JN331_001052"
     CDS             complement(22570..23583)
                     /gene="argF"
                     /locus_tag="JN331_001052"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY7259254.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
     gene            23745..24161
                     /gene="rraB"
                     /locus_tag="JN331_001053"
     CDS             23745..24161
                     /gene="rraB"
                     /locus_tag="JN331_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY7259255.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(24207..24710)
                     /locus_tag="JN331_001054"
     CDS             complement(24207..24710)
                     /locus_tag="JN331_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7259256.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            24903..26099
                     /locus_tag="JN331_001055"
     CDS             24903..26099
                     /locus_tag="JN331_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY7259257.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMK
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            complement(26153..29008)
                     /gene="valS"
                     /locus_tag="JN331_001056"
     CDS             complement(26153..29008)
                     /gene="valS"
                     /locus_tag="JN331_001056"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY7259258.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENHESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(29008..29451)
                     /gene="holC"
                     /locus_tag="JN331_001057"
     CDS             complement(29008..29451)
                     /gene="holC"
                     /locus_tag="JN331_001057"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY7259259.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(29585..31096)
                     /gene="pepA"
                     /locus_tag="JN331_001058"
     CDS             complement(29585..31096)
                     /gene="pepA"
                     /locus_tag="JN331_001058"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY7259260.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRARFNGEE"
     gene            31363..32463
                     /gene="lptF"
                     /locus_tag="JN331_001059"
     CDS             31363..32463
                     /gene="lptF"
                     /locus_tag="JN331_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7259261.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            32463..33545
                     /gene="lptG"
                     /locus_tag="JN331_001060"
     CDS             32463..33545
                     /gene="lptG"
                     /locus_tag="JN331_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7259262.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(33664..35166)
                     /locus_tag="JN331_001061"
     CDS             complement(33664..35166)
                     /locus_tag="JN331_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY7259263.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(35295..36314)
                     /gene="ahr"
                     /locus_tag="JN331_001062"
     CDS             complement(35295..36314)
                     /gene="ahr"
                     /locus_tag="JN331_001062"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY7259264.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            36510..36594
                     /locus_tag="JN331_001063"
     tRNA            36510..36594
                     /locus_tag="JN331_001063"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36544..36546,aa:Leu,seq:caa)
     gene            36793..38023
                     /locus_tag="JN331_001064"
                     /pseudo
     CDS             36793..38023
                     /locus_tag="JN331_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         9137 a         9445 c         9796 g         9645 t
ORIGIN      
        1 gtgtggagag gcgcaggaga ttacgcgtcc gggaactcac ggataaagcg ttcgacatct
       61 tcaaccatat ggtcgttgcc gacaaagaac gaacggcgct ggtgcagagt ttccgggatg
      121 atatccagaa tacgctcttt gccatcgctc gctttaccgc ccgcttgttc cgccaggaat
      181 gccatcgggt tgcactcata aagcaaacgc agtttgccgt ccgggtggct ggcggtgctt
      241 gggtagagat aaataccgcc tttcagcagg ttacggtgga aatccgcaac cagtgaaccg
      301 atataacgcg aggtatacgg gcgattggtg gatttatctt cttcctggca gaatttaatg
      361 tacttcttca ccccgttcgg aaacttaatg tagtttcctt cgttgatgga gtaggttttg
      421 cctttctccg ggaagcgcat ccgttcctgg cacaggcaga aaacgcccag cgaaggatcg
      481 taagtaaagg cgtgaacacc gcatccggtg gtgtaaacca gcatggtaga ggagccgtat
      541 accacgtaac ctgccgcaac ctgtttgtta ccaggctgga ggaaatcttc ttccgttacc
      601 ggcgtgccaa caggcgtaac gcggcggtag atggagaaaa tggtaccgac agagacgtta
      661 acatcgatgt tggacgagcc atccaggggg tccatcagca ccacgtattt tgcgtgttca
      721 cagccttcaa agacgacaat ctcatcttct tcttcagagg caatccccgc aacgatatcg
      781 cgtgctttca gtgcggcttt cagtttttca ttagcgaaca agtcgagttt ttgctgaacc
      841 tcgccctgca cgttctcagc accgctggca cccaggatat caaccagtcc tgctttgttg
      901 atatcgcgat ggataatctt ggcgcccagt tttattgccg acagcaaagc agtgagctca
      961 ccggtagcat gagaaaactc gtgctgcttt tcgacaataa attcacctaa cgttttcata
     1021 aaactttccc tgcaatgttt atggagtaaa gcgaccgcaa caatcttaac aaataatctc
     1081 aatgttgcgc tcaggtgaat cgcgccagca aattgcggat tatcctgaaa tgcgtttctc
     1141 acttgcccga catatgcgta aaatgagcgg cagattaaaa aaggatagtg acgtatgcgc
     1201 attcatattt taggaatttg tggcacattt atgggcggtc tggcgatgct ggcgcgccag
     1261 ttaggccatg aagtaacggg ttcggacgcc aatgtgtatc cgccgatgag caccttactt
     1321 gagaagcaag gcattgagct gattcagggt tacgatgcca gccagctcga gccgcagccg
     1381 gatctggtga ttattggcaa cgccatgacc cgtggaaatc cgtgtgtgga agcggtactg
     1441 gaaaaaaaca tcccttatat gtcaggtcca cagtggctgc acgattttgt gctgcgcgac
     1501 cgctgggtgc tggccgttgc cggtacacac ggcaaaacca ccaccgcggg aatggcgacc
     1561 tggattctgg aacagtgtgg ttacaaaccg ggatttgtga tcggcggtgt gccggggaac
     1621 tttgaggttt cggcgcgtct gggcgaaagc gacttctttg ttatcgaagc ggatgagtac
     1681 gactgcgcct tcttcgacaa acgctctaaa tttgtccatt actgcccgcg tacgctgatc
     1741 ctcaacaacc ttgagttcga tcacgccgat atctttgacg acctgaaagc gatccagaaa
     1801 cagttccacc atctggtgcg tatcgttccg gggcagggcc gtattatctg gccggaaaat
     1861 gacatcaacc tgaaacagac catggcgatg ggctgctgga gcgagcagga gctggtgggt
     1921 gagcagggtc actggcaggc gaaaaagctg accaccgatg cttccgaatg ggaagttttg
     1981 ctggatggcg aaaaagtggg cgaagtgaaa tggtcgctgg taggcgaaca taatatgcac
     2041 aatggcctga tggcgattgc agcggctcgc catgttggtg tagcgccggc agatgccgct
     2101 aacgcgctgg gttcgtttat taatgctcgt cgccgtctgg agttgcgtgg tgaagcgaat
     2161 ggcgtcacgg tatatgacga ttttgcccat cacccgacgg cgattctggc aacgctggcg
     2221 gcgctgcgtg gcaaagttgg tggtacggcg cgcattattg ctgtgctgga accgcgctcg
     2281 aataccatga aaatggggat ctgcaaagac gatctggcac cttcattagg tcgtgccgat
     2341 gaagtcttcc tgctgcaacc ggcgcatatt ccgtggcagg tggcagaagt ggcagaagcc
     2401 tgcgttcagc ctgcacactg gagtggcgat gtggatacgc tggcagatat ggtggtgaaa
     2461 accgctcagc ctggcgacca tattctggtg atgagcaacg gcggttttgg tgggatccat
     2521 cagaaactgc tggatggtct ggcgaagaag gcggaagctg cgcagtaatt cggcttcagc
     2581 ctgatatagc attgccggat aaggcgttta cgccgcatcc ggcaaaccat actcacatca
     2641 acaacgaaaa ttacccttca ttctccgcca gttcgcgcag atactggaaa atctggcgtg
     2701 cggatttcgg cggcttattc ccttctttct ctttcttcgc gttgcggatc agtgtacgca
     2761 gttgctgacg atcggcatcg ggccacagat tcaatacttc agcgatggcg tcatcacctt
     2821 gatcgatcag acgatcgcgc aggttttcca gtttatggaa cagcaccacc tgctggttgt
     2881 gacggttttt cagcttatcc agcgcctgac gaataggctc tacgtcgcgc tggcgtagca
     2941 tcttaccgat gagctgcaac tggcggcggc gaccttccat cttaatacgc tgggcgagct
     3001 caatagccgg gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt ttccccagat
     3061 caacaatttc cgcgccaagg cgttttagct cctcggcatc acgtttaatt tcacttttac
     3121 tgacccagat aatttcatcg tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc
     3181 agtcttcggg ctgcttagtc atctcaggct ccttaaaaaa agaggctaat gttaccagtt
     3241 aagatgcgca ctgaaaaacg gttctctgtt agacttcaga gaaactctct acattatggc
     3301 acttgcaatg aaagtaatct ctcaagttga agcgcagcgc aagattctgg aagaagcagt
     3361 ttccactgcg ctggagttgg cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa
     3421 gaccaccggc attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg aattcaatag
     3481 tgatggcgcg ctggggatca ctgtttatca ccagaaccgt aaaggtagcg catcgtctac
     3541 cgatttaagc ccgcaggcca ttgcccgcac cgtacaggcg gcgctggata ttgcccgtta
     3601 tacctcgcca gatccctgtg ccggcgtggc agacaaagag ctgctggcct ttgacgcacc
     3661 gaatctcgac ttgttccacc ctgcggaagt ttccccggat gaagccattg aactggcggc
     3721 ccgcgcagaa caggcggcat tgcaggcgga caaacgcatc accaataccg aaggtggcag
     3781 ctttaacagc cactacggtg tcaaagtttt tggcaacagc cacggcatgt tgcagggtta
     3841 ctgctcaacg cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg gcgatatgga
     3901 gcgtgattac gcctacacca ttggccgcgc gatgagcgat ctgcaaacgc cagagtgggt
     3961 cggggccgac tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac tctccaccat
     4021 gaaagtgccg gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc atctggtggg
     4081 ggcgatagcg ggtggctcgg tttaccgtaa atctaccttt ctgctggatt cgctgggtaa
     4141 acaaattctg ccggactggc tgaccattga agagcatccg catctgctga aagggctggc
     4201 gtcgacgcca ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg
     4261 catcctgact cagtggctgc tgaccagcta ctcggcgcgg aaactggggc tgaaaagcac
     4321 cggacatgcg ggcggtattc acaactggcg gattgccgga caagggctaa gcttcgaaca
     4381 aatgctcaaa gagatgggca ccgggctggt ggtgacggag ctgatgggcc agggcgtgag
     4441 tgccattacc ggtgattatt cccgtggtgc agcgggcttc tgggtagaga atggcgaaat
     4501 tcagtatccc gttagtgaaa tcaccatcgc aggtaattta aaagatatgt ggcgcaatat
     4561 tgtcaccgtc ggtaacgata ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt
     4621 gccggagatg aaaatcgccg ggcagtaaaa aactggcgcg acctgccgcg ctataaacta
     4681 aaaaattaca caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag
     4741 cgtactcgca gtacgtgacc cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg
     4801 atgaagtgta aataaaaaag gaagtgagca atgcgtaaaa gcctgttagc tattcttgca
     4861 gtctcctcgt tggtattcag ttctgcgtcg tttgccgctg atcttgcaga caatatggaa
     4921 accctcaacg acaatttaaa agtgatcgaa aaagcggata acgcggcgca agtcaaagac
     4981 gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc gccgaagctc
     5041 gaagataaat caccggacag cccggaaatg aaagatttcc gccacggttt cgacattctg
     5101 gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa agaagcgcag
     5161 gctgctgcag agcaactgaa aacgacccgc aacgcctatc accagaagta tcgttaattc
     5221 ctcatttccc tgttgcctgc actcaggtaa cagggaactt atcatcgcaa atgatgcacc
     5281 acctgattgc tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg cacaaatttg
     5341 ccgtcgacca gcacgttaat caaatcaaca acctgcattt gcgcagcgtt gagttcgtcg
     5401 agtttatagc ctgtccacac ccagatgtct ttacccggac actcggcgcg gatgcgtttt
     5461 accagtttca gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc ggagagggaa
     5521 atcccctggc gtttgatacg agtgtcattc agatctttaa tgatcttgtc ttccattgct
     5581 ttggtaaatg gctgaccaga atttacccgc catgtgcttt tgttatagca accggggcat
     5641 tcatgaacac acccggagac aaacagggtg cagcgagtgc cggggccgtt gacgatgtcg
     5701 acaggatagt actgatgata attcattatt tcgcctggct tatcagtccg cgtgtaggct
     5761 gcatccggta atcaatgcct gatgcgacgc tgtcgcgtct tatcaggcct acagccgttg
     5821 ccaaatgtag gccggataag gcgtttacgc cgcatccggc atttgcttag cgcagagtga
     5881 agattaacct atctgcccat tccccaaatg tttaacgcgg cgcttaactt cttcctgctt
     5941 accagcgtta aacggacgtg catccgggct acctaaatat ccgcacacgc ggcgagttac
     6001 cgacacacgg gaggcgtcat ggttaccaca tttcgggcaa gtgaagcctt tgctggtgca
     6061 ctcgaactca ccggtaaagc cacactcgta gcactcatca atcggtgtat tggtgccgta
     6121 atacggtaca tgctgatagc tgtaatccca gacatcttcc agcgctttca ggttgtgctg
     6181 aatgtttgga tactcgccgt agcaaatgaa accaccgttc gccagcggcg ggtaaggcgc
     6241 ttcaaagtcg atcttgtcgt acgggttcac cttcttctcc acatcaaggt ggaaactgtt
     6301 ggtgtagtaa cctttatcgg tcacgcccgg caccacgcca aactcagcag tatcgagacg
     6361 gcagaagcgg tcgcacaggt tttcactcgg cgtgctgtag agactgaaac cataacccgt
     6421 ttcttctttc cactgatcca ctgcctgacg cagacgttca acaatggcga tacctttcgc
     6481 gcgaagcttc tcgttgtcgt aaacatgctc gccggcgaac agcgcgttaa tggtttcgtg
     6541 gatgccgatg taacccagcg aaatagacgc acgaccgttt ttgaagattt cagaaacatc
     6601 atcgtcagca ttcagacgta cgccacaagc accttccata tagaggatcg gggcgacgcg
     6661 cgctttcacg ccttcgagac gagcgatacg ggtcatcagt gccttacgtg ccagcaccag
     6721 acgttcatcc agcagcttcc agaaggtggc ttcatcgcct tttgcttcca gagcaatacg
     6781 cggcaggttc aggctgatca cgccgaggtt gttacgacca tcgtggatct gctcgccgtt
     6841 ttcattttcc cacacgccga ggaagctgcg gcagcccatc ggggttttaa acgaaccggt
     6901 gactttcact acctgatcgt agttcaggat atccggatac atgcgcttgc ttgcgcactc
     6961 cagcgccagc tgtttgatgt cgtagttcgg atcgcctttt ttatggttca ggccatcgcg
     7021 aatcgcaaac accagtttcg ggaacaccgc agttttacgg tttttaccga ggcctgcgat
     7081 acggttacgc aggatcgatt cctgaatcag gcgcgattcc cagctggtgc ccagaccaaa
     7141 accaaaggtt acaaacggcg tctgaccgtt ggcggtgtgc agggtgttta cttcgtactc
     7201 cagcgactgg aaggcatcgt agcactcttt gatggtacga gagttagcgt agccttcggc
     7261 gtccgggatg ttccactctt ctgcggtctt gcgatgtttg ttgtagctgg cggtaacaaa
     7321 cggcgccagc acttcatcga tacggttaat ggtggtgccg ccataaatat ggctggcgac
     7381 ctgagcaata atctgcgcag ttaccgcggt tgccgtagag atcgacttcg gcggttcaat
     7441 ctcggcgttc cccattttaa agccctgggt cagcatgcct ttcaggtcga tcaacatgca
     7501 gttgaacatc gggaagaacg gcgagtaatc gagatcgtga tagtgaatat cgccacgctc
     7561 atgtgcctgc accacgtcac gcggcagcag gtgctggcgt gcatagtgtt tagccacgat
     7621 cccggccagc aggtcgcgct gggttggaat caccttgctg tctttgttgg cgttttcgtt
     7681 gagtaacgag gcgttggtct gctcgaccag accacggatc tcctggttca ggcgaccgcg
     7741 tttttcacgt tcaatgtcgc gatcgtgacg gtactcgatg taagcacgag ccagttgttt
     7801 gtatggaccc gacatcagct ggttttcaac tgcggtctgg atctcgttga tatccacctg
     7861 gttgcggccc tgcatctgct cgctgacaac cgcggcaaca gtggcgcaat aatcggcatc
     7921 atcgacttcc gctgctttag ctgcacgcag aatcgcttct ttgatgcgct ctgatttaaa
     7981 cggcactttg cagccgtctc gtttcatcac atgcggtgtc atgatcgctc catattttta
     8041 agaacaggtt atccacagaa attgggaaag gcgttttccg gttttttcgt ttcgttttcc
     8101 ggtgctttcc gcaatcgcca tccgcttatc cacaacgccg cacctttttc gtggggagct
     8161 gtagtagcaa ttatagtcga ttaatacaac atattgggtt gggacgcatt ttaaagtcta
     8221 tatatagtgc tttgcatcaa ggatgtttta actttttttg atgtagctca aagtaaaaag
     8281 cagagcgtac ggatgacggg cgctacagcg ttatgtaaat tttttaatga atttgctggt
     8341 tgaaaaatca acaaaaacaa catgctgaca gacaaaaccc cggaatgacc ggcattatcg
     8401 gggcttaggg aagatttact tctgtaacca ccagacagcc tcaaaaggcc gtaaattcat
     8461 ggcacagggt tgtggtgagg cttcttcgta gttatgcatc acaagctgcc agttgccgcg
     8521 catttgccct ggctgccagg gttggatctc acggctaagg ttggcaatga ccagcaaggt
     8581 ttgccccttc cattcacggc gatagcacca caatacaggg ctgtttggca gcagatcctg
     8641 gtaattgccc catgtcagga tggcttcttg cttacgcagt gcgattaact tttggtaggt
     8701 gtaaaacaca gaggagtcat cggccagcgc ggcttctacg ttgatttgtt gatagttatc
     8761 gcctaggcca atccacggtt cgccagccgt aaacccggca ttatcgccgt tgctccattg
     8821 catgggcgtg cggctgttgt cacgggattt actggcgagg attgccaata actcgtcggc
     8881 atcacgacca tcgttgcgca gctcggcaaa catattgagg ctctccacgt cgcgatagtc
     8941 agtaatgcgc gtgaaatgcg ggttggtcat gccaatctct tcgccctggt agatatacgg
     9001 cgttccctgc atgccatgca gcaccatcgc cagcattttt gccgcaggca cgcggtattc
     9061 accttcatca ccaaagcgag aaacaatgcg cggctgatcg tggttacacc agaacaaggc
     9121 attccatgcc ccgttgtgca ttccttgttg ccagtggcgg aacaatgttt tcaacgccac
     9181 aaagtcaggt ttagccagcg tccatttttc accaccggga taatcgacct tcaggtgatg
     9241 aaaattaaag gtcatcgaca attcactgcc tgtcagtgcc gcgtatcgct ggcaatgctc
     9301 aaggctggtg gaggacattt cacctacggt cattaacccg cgtggcgtaa acacatcgcg
     9361 gttcatctcg tgcaaaaact cgtgtgctcg tggcccgtcg gtgtagaagc gacgcccgtc
     9421 gccatccagg tcttcaggga aacacgggtc tttggagatc agattcacca catccaggcg
     9481 caacccgtcg accccacgat cggcccagaa ttcacagact tttttcagct ctgcgcgtac
     9541 cgctggattc tcccagttga gatccgcctg ttctggtgca aagagatgca aataatactg
     9601 ttcgctttcc gcatgccagc gccacgcact accgccaaat tttgaacgcc agttgttcgg
     9661 tggcgtttct ggttcgccat cgcgccagat ataaaactgg cggtaagggc tttctttgtt
     9721 cagcgcctcg cgaaaccagg catgttgggt agaggtatgg ttaaacacca tatcgagaat
     9781 gatacgaatc ccgcgcgatt ttgcctgcgt caccagttcg tcaaaatcgt ccagcgtgcc
     9841 gtaggtggga tcaatcgccg tatagttcgc tacatcgtaa ccgttatcga cctggggaga
     9901 gacataaaag ggggttagcc agatggcatc aacgcccagt ttatgcagat agtccaggcg
     9961 ttggataacg ccacgtaaat cgccggtgcc gctacccgtg gtgtcctgaa aactctttgg
    10021 ataaatctgg tagataacgc cgttttgcca ccagtgggga agattagtca ttacgttatt
    10081 cctgcaaatg cgagggggcg caattgcgcc ccgaagaaaa ttaaacaatg tccagcgtgc
    10141 ccaggcggta tttccgctga tagataaacg aggtgagtac aatcgggatg atgatggcga
    10201 tagccattgc cagcgcaaat acctgccagt agctcggttg aatcgagaga attcccggca
    10261 ggccccctac gccgatacca ttcgccataa cgccgttcag gccgcatagc aatcctgcca
    10321 gaccagaacc aatcatcgcg cacagcatcg ggaagcgata tttcaggttg atgccgtaca
    10381 ttgcaggctc agtgacccca agccaggcgg agatagcggc aggcacggag atctcgcgtt
    10441 cattgtgctt gcggctggaa atgataatgc ctatcacggc ggagccctga gcgatattcg
    10501 acagcgcaat cagcggccac actggcgtgc cacccatgct ttgaatcatc tgcaaatcaa
    10561 tagcaagggt ggtctggtgt acgccggtga tcaccagcgg cgcgtacagg aagccaaaca
    10621 atgccgcacc aatcggagca aagctgccgg tcatcaggtg acgtaccgca aaggcaacgc
    10681 catcgccaat catgcgacca aacggaccaa tcagcgcatg ggcgaggaac accgcgagga
    10741 tcagcgaaca gacgggcacc accaccagat agaggtaatc cggtacgatg cgtttaaggc
    10801 gagtttcaat aacgcccagc gccagcccgg ctaacagtgc cgggatcacc tgcgcctgat
    10861 agcccacttt ggcgatgctg aacatgccaa agtcccacac ttccggcagc tgctgcccga
    10921 gcagataagc gttcatcagc tggggggaaa ccagtgtcac gccaagcacg ataccaagga
    10981 tcggcgtgcc gcccattttt ttcaccgctg accagcaaat cccgaccggc aggtagaaga
    11041 aaatcgcttc accgatcaac cacagaaaat cgtagatcgt ttgcagggaa gggtacattt
    11101 gcgccagcgt ctgaccgttg ctcatgggca aatcgccgat cacattgcga aaaccgagga
    11161 tcaaaccgcc gctaatcaac gcgggcagca acgggaagaa gatctccgcg aaatgagaga
    11221 tcaactgctc atgccatttc atattttgcc gggcggcttt ttttacctgc tctttatcaa
    11281 cctgcgcctg tccggttgac gctattagtg cttgatagta atcacccacg ttggtgccaa
    11341 tcaccacctg aaattgcccg gcattggtga aacagccttt caccatgggg agttgctcaa
    11401 tttctttcgg tctggcattg gccggttggt tgaggacaaa gcgcaggcga gtaatacagt
    11461 ggctcaccgt cgcaatattg ccgcgcccgc cgaccagttc aatcaaccga tcgatatccg
    11521 tttggtttat tttgctcatc ataaagcccc atggcagatg acatttttgg ttggctgcag
    11581 aatatattgc gcggaaaaat ttaaaaacgg gaacgttccc gaaacgcagc gaagatcaca
    11641 atttatcgtt caggaaacga tcaggacagg gtggcgggga tgatgatttg ttgcggttcg
    11701 ctgcgcccgg ttacctgtgc gatcaattgg caggccgcct ggcgtccagc ttcggcgtag
    11761 ccaggatcta cggtgacgat ctccggatga aggaatttca ttaacggcgt attacctacg
    11821 ctcgccagtt gcaaggtgtc gatgcgttgc tcttgcaggt atttacttgc gccaagagcc
    11881 agcgtgtcgg ttgcgcacag taacgcggta gtttcaggcg taatcacttt tgctacgttt
    11941 tcatagcctt gcttcatagc aagccctggc agagcggcaa cgggatgcag tttatgcgct
    12001 ttgcagaacg ccaggtaggc ttcgtgacgt cgcttcccgg tcgtcacgtc actatgcggc
    12061 acgccgagat aactgatatt gcgatgcccc tggtcataga gccgttgcat cagaatcttg
    12121 attgcccctt cgtcgtcata acagaccgaa gcaaagcctt ttgcgtcacg cgccagcaga
    12181 accagcgatg actgccagtg ggctaacatt tcttcggtta tgccggtaaa accgaacagc
    12241 actacgccgt cgatattacg ccgtttcagc acccccaaat gttcggcaac taattgtggg
    12301 gaaaactgat tttccatcat gattggatcg taaccttgtt catagaacgc tggcagcatg
    12361 gtttgaacgg cgagattttc tgacaacgaa tccagacggg taacaatgat ggcgaccact
    12421 ttatcgcttt gcccacgcat agcgcgcgca gagcgggaag gggaaaatcc atgctgattc
    12481 atcactgctt caacacgctc gcgcgtgcgc tgactcacgc cgctttcgtt attcagcacc
    12541 cgggaaaccg tagatttccc cacgccgctt aagcgcgcga tgtctttgat ggtcagccga
    12601 ttttgcatcc tgttgtccca taacgtgttg tttaattatt tgagcctaac tttacccgtg
    12661 cattcagcaa tgggtaaagt ctggtttatc gttggtttag ttgccagcag gtattatatc
    12721 gccataaatg ctacgaatat tattggattc tccttattat ttgtggcgct tttttcactt
    12781 accggaggtt atatggaacc tgatcccacg cctctccctc gacggagatt aaaacttttc
    12841 cggtaagccc gtcttttcac ggcgttaccg gatgcgtaag gccgtgacgt tttaatgtcc
    12901 ctgctcagct ttattacctt caggtaaggc ttcgccacgc ctgaagacat ttctgtactg
    12961 tttcagacag tgcggaggga ctccttatgt ttaaagaaat ttttacccgg ttcattcgcc
    13021 atttaccttc ccgtctggtt catcgcgatc cattgcctgg cgcgcagcag acagtgaata
    13081 cggcggtccc gccgtcctta agtgcgcatt gcctgaaaat ggcggtgatg cccgaagaag
    13141 aattgtggaa aacgttcgac acccatccgg aaggattaaa tcaggcggaa gtggaatctg
    13201 cccgcgaaca acatggtgat aataaattac ccgcacaaca accgtcgccg tggtgggtac
    13261 atttatgggt ctgctatcgc aaccccttta atattttgct caccattctt ggtgctattt
    13321 cttacgccac ggaagattta tttgccgcag gtgttatcgc gctaatggtc gctatttcta
    13381 cgttgctgaa ctttattcag gaagctcgtt ccaccaaagc ggcagatgcc ctgaaagcga
    13441 tggtcagtaa taccgcgacg gtactgcgag taattaacga caaaggcgaa aatggctggc
    13501 tggagatccc gatagaccag ctggtgcccg gcgatattat aaaactggcg gcgggggata
    13561 tgatcccggc agatttacgt atcttgcagg cgcgggatct gttcgtcgct caggcgtcgt
    13621 taaccggtga gtctctgccc gtggaaaaag ccgctactac tcgccagccg gagcacagca
    13681 atccgctgga gtgcgacacc ctgtgtttta tgggcaccac cgtggtgagc ggcacggcac
    13741 aagcaatggt gattgctaca ggagccaaca cctggtttgg tcaactggcg gggcgtgtta
    13801 gtgagcagga aagcgagccg aatgcctttc agcaagggat cagccgcgtt agtatgctgc
    13861 tgattcgctt tatgctggtg atggctccgg tggtgttgct tatcaacggt tacaccaaag
    13921 gcgactggtg ggaagcggca ctgtttgcgc tttcggtcgc ggtaggctta acgccggaaa
    13981 tgttgccgat gattgttacc tcgacgctgg cgcgcggcgc agtaaagctg tcgaaacaga
    14041 aagtgatcgt caaacatctg gatgccattc agaactttgg cgcaatggat attctgtgca
    14101 ctgataaaac cggcaccctg acgcaggaca aaattgtgct ggagaatcat accgatatct
    14161 ccggtaaaac cagcgaacgc gtgctgcata gcgcgtggtt gaacagtcat taccagaccg
    14221 gacttaaaaa tctgcttgat accgcggtgc tcgaaggtac ggatgaagag tcagcgcgtt
    14281 cgctggccag ccgttggcaa aaaattgatg agattccgtt tgatttcgag cgtcgtcgga
    14341 tgtcggtggt agtggcagaa aataccgaac accatcagct ggtttgcaaa ggtgcattgc
    14401 aggaaatcct caatgtgtgt tcgcaggtgc gtcacaatgg cgagattgta ccgctcgatg
    14461 acaccatgct gcgtaagatt aagcgggtta ctgatacgct gaatcgtcag gggctgcgcg
    14521 tggttgcggt ggcgacgaaa tacctgccag cgcgtgaagg agattaccag cgggcggatg
    14581 aatccgacct gatcctcgaa ggatatattg ctttccttga tccgccaaaa gagacaaccg
    14641 ctccggcact gaaggcatta aaagcgagtg gaattaccgt aaaaatcctt actggcgaca
    14701 gtgagttagt tgcagcgaaa gtgtgccatg aagtggggct ggatgcggga gaggtggtca
    14761 ttggtagtga tattgaaacg ctatctgacg atgaactggc aaatctcgca cagcgcacca
    14821 cgctgtttgc ccgcctgacg ccgatgcata aagaacgcat tgtgacctta ctgaagcgcg
    14881 aagggcatgt ggttggcttt atgggcgatg gtattaatga tgcgcccgcc ttacgcgctg
    14941 cggatatcgg catttctgtg gacggcgcgg tagatattgc ccgtgaagcg gctgatatca
    15001 tcctgctgga aaaaagcctg atggtgctgg aagagggggt tattgaggga cgtcgcactt
    15061 tcgccaacat gctgaaatac atcaaaatga cggcgagttc taacttcggt aatgtgttca
    15121 gcgtgctggt ggcgagtgct ttcttgccct tcctgccgat gttgccgtta cacttgctta
    15181 ttcagaacct gctgtacgat gtgtcacagg tggcgatccc gtttgataac gtcgacgacg
    15241 agcaaattca aaagccgcag cgttggaatc cggcggatct ggggcgcttt atggtcttct
    15301 tcggaccgat cagctcgatt ttcgatattc tgacgttttg cttgatgtgg tgggtattcc
    15361 atgccaacac gccggaaacg caaacgctgt tccagtcggg ctggtttgtg gtgggattac
    15421 tgtcgcaaac gctgattgtg catatgatcc gtacccgccg tgtgccgttt attcagagct
    15481 gtgcatcgtg gccgttaatg atcatgaccg tgatcgtgat gattgtcggg atcgcattgc
    15541 cgttttcacc gctggccagt tatctgcagc tgcaggcgct gccgttaagc tatttcccgt
    15601 ggctggttgc gattctggca gggtatatga cgttaaccca gttggtgaaa gggttctata
    15661 gccgtcgtta cggctggcag taaagaataa actgggcatg gtggcccgga ttcagattgc
    15721 agacaaagtg agctttgttc atgccggatg cggcgtaaac gcccaatccg gcctacaaat
    15781 tcaataaatt gcaggaatca cgtaggcctg ataagcgtag cgcatcgggc gattttgctt
    15841 ttgtcatcag acttaatccg ggcatgatag cccggatttc catcaagatt agcgacgaac
    15901 agcgattgct tcgatctcaa tcttcacgtc tttcggcaga cgggcaactt caacgcaaga
    15961 acgtgccggg aaagtggcgt tgtgttcggt gaagaaggct tcgtaagtgg cgtttacggt
    16021 tgcgaagtcg ttcagatctt tcacgaacac agtggtttta acgatgtcgc ccactttcag
    16081 gccagcggct tcgacgattg cttttacgtt atccagcgac tgacgtgcct gtgcagcgac
    16141 gtctgccggt acttcaccag ttttcggatt taccgggatc tgaccggagg tgatgatcat
    16201 attgcccaga tcaacgccct gtacgtaagg accgatagct gccggtgcat tttccgtcgc
    16261 gatagttttg ctcatgattt ctcctttatt acagcggtaa aaagtctggc tcattatagg
    16321 gagccagact tttattacca accgcaatta attggccagc accacattat gggaaaactc
    16381 tttttcacag tatttgcatt taagcgcgat gtcattggcg cgttttcgca cggcaaagct
    16441 ggatgaaacc ggttcggcat ggctgataca gttgctgttc gggcagacca gcacattgtc
    16501 gatgcgctcc ggcagacttg ggcgcgattt acccaccact tcatagttgt cgatacggtt
    16561 aaccgtggct tgcggtgcat acaatgccag ttggtctact tgatcttcac tcaaaaaggt
    16621 attttcgatt ttgatcagat ctttgcggcc catctcgcca gaaggcaaat tcagaccaat
    16681 ggtgatgcgc tgatctgttt cggtcaactt gaacagactc aacagcttaa aaccgatctg
    16741 ggcggggata tggtcaatta ccgtgccgcg tttaatagct tcaacctgca atttattatc
    16801 gtgtgtcatc tctatttccc cttacagtac cagatcgcga ttcagaacca gtgccagtaa
    16861 cgcctggcga gcgaaaattc cgttgcctgc ttgctggaag taccaggcgt gaggcgtttt
    16921 atccacatcc gtcgcaatct catcaacacg cggcagcgga tgcagcactt tcatattggc
    16981 tttggcgttg tggagatcgc tggcgcgaag aacaaactgc gctttcacgt tggcgtactc
    17041 ggacgggtcc agacgctctt tttgcacgcg ggtcatgtac aggatgtcta cttccgccat
    17101 cacttcttca atagagctgt gcagactcca ggcgatccct ttttcatcga gcatatccag
    17161 aatgtattgc ggcattgcca gcgcatccgg cgcgataaag taaaaacggt tgccgtcgaa
    17221 cttcgctagc gcctgggtca gggagtgaac ggtgcggcca tatttcaggt caccaaccat
    17281 tgcgacgtgg agattgtcca gacgcccctg ggtttcctga atggtgaata agtccagcaa
    17341 ggtttgcgtc ggatgttggt tggagccatc accggcattc agtaccggta cattgccaga
    17401 aaattcggtg gccaggcgcg ccgcaccttc ctgcggatga cgcatcacta tcgcatcgac
    17461 gtaagtgctg ataaccgaaa tggtatcggc cagcgtttcg ccttttttac ccagtgatgt
    17521 attggcgctg tcggagaagc ccaccacgct ggcacccagg cggtgcatgg aagtttcgaa
    17581 agagaggcgg gtacgggtag aggcttcgaa gaaacagctg gcaatgactt tgtgcttcaa
    17641 cagctctggt tgcgggtttg ctttcagttt cgccgctgtc gccagcacca gattaaggtc
    17701 atcgcgacta aggtcgttta tggaaatgat atgtttctga tatagcggat tagccatctt
    17761 ttatctcctg acgcctaggc aaaaaaaagc ccctcgattg aggggctggg aatgggtgat
    17821 caacgggaag aaaaacggca ggccagcgtc ttttttcaga cgcggtaaga caaaatgtcg
    17881 aacacactga accatacatc ctcccggcaa attgtccggc attatactca tagccagaag
    17941 cggtgcaagc atttgatgca atattttgtc agcgcaaacg gtttatttga attaaaagtc
    18001 aaaatatatg catttttatt ttctatgatt ctggagggga agtgtcgttc ggtatcaata
    18061 attgcagaca tttcctgctt ttttcttttt cattttcacg caatcagaaa tgcattttat
    18121 tccattcatc cgacttatag ggcggagggt gaaaacgaac ggctaacact attgcttata
    18181 gctcagggat acgcgctatc actttaattt caaaatcaaa gcctgccagc caggtaacac
    18241 ccaccgccgt ccagtttgga taaggtgggg cgctaaatat ttcatttttc accgtcatga
    18301 tgtcttcaaa ttgtttttcc ggatcggtat gaaagctcgt aacatcaatg atatcgtcaa
    18361 aagtgcatcc cgcagctgcc agggtcgcat gcaaattatc aaaggccagt ctgacttgtt
    18421 gctgaaaatc gggttctggt gttccgtcct ctcgacttcc tacttgcccg gaaacaaaca
    18481 gcaaatcgcc ggaacgaata gccgcagaat aacgatgctc agcatacagt gaatgtcggc
    18541 cagcagggaa aacagcggtt ctttctacca tttggttatc ctcaagattt acgacatgaa
    18601 cagaagattt atatttaccg ggagccgctt ttagggggac gacgttagta aacaaaaccc
    18661 agacatcatg gataatggct gggcttaatt gagcgtagtc ggttatgcgc caaacgcgcc
    18721 atcaatggta tgcatcgcgc cggtaacaaa actggcttct ggccctgcta accatgcgac
    18781 cataccagcg acctcttccg gttgcccatg tcttttgatg gccataaaac tatgcaacat
    18841 atcgcgtatt ggcccgttgg cgggattaac gtcggtatca attggtcctg gctggacgac
    18901 gttgatggtg atccctcgcg gtccaaaatc acgggccagc ccgcgcgcca tgccttgcag
    18961 ggcagattta ctggcggcat aagcagccat gcctgcaaca ggcatacgat cgccattcac
    19021 ggagccgatg attaagatgc gcccgccttc gggcatctgc cgggcggctt caacagaggc
    19081 atgataagga gcatgaatat tgattttgaa aaggcgatca atatcgtcgg catttaattc
    19141 cagggcatcg ccaaagacgc caatacctgc atttaccacc aggatatcca atgtgccgct
    19201 cttacgaacg acatcaataa cagcgtctct gtcagcacta tctgtgaata ctgctgtcgc
    19261 tccagtctct tgtgccagac gttcagcggc atctttcgac cccgcatagg tgaatcgtac
    19321 attggcccca tcggtgacga aacgacgtac gatagcggca ccgattccac gactgccacc
    19381 gaggatgaga actgtcttac cggtaaaagc gcccataagg actccttgat ttattatgta
    19441 acatgcatta caaaactgtt ttaactttct gtcaacaagt tttgtaatgg tcactaaaaa
    19501 acaatctcgc gttccaggtc gtcccagacg tttcgctcct gagcaggcag tctctgcggc
    19561 aaaagtgctt tttcaccaaa aaggtttcga tgcagtaagt gtctctgaag ttactgatta
    19621 tcttggtatt aaccccccga gcctctacgc ggcttttggc agtaaggctg ggttatttag
    19681 ccgtgtactc aatgaatatg tcggtacgga agctattccg cttgccgata ttcttcgtga
    19741 tgatcgtccg gtaggcgagt gcctggttga ggtattaaaa gaagcggcgc gcagatatag
    19801 ccaaaacggc ggctgcgctg gctgtatggt tcttgaaggt attcatagtc atgatccaca
    19861 agcgcgtgat attgccattc aatattatca cgccgcagaa acgaccattt atgactatat
    19921 cgccaggcgg catccacaaa gcgcacaatg tgtgactgat tttatgagta ccgtgatgtc
    19981 agggctttct gcgaaggcac gggaggggca ctccatcgaa caactctgtg caacagctgc
    20041 cctggcgggg gaagcgataa aaactcttct caaggagtga tgctggcctt gatccgaaag
    20101 gcgggaacgc gcctgccgat aagttgtgat aagacaataa ttcacgcgtt aaggctggcg
    20161 gaattgatca tcttttcgta taacgataga aatgaaacat tgttttaatt aaggagtgga
    20221 aaagatgatc atcggaaata ttcataatct tcagccgtgg ctaccccagg agttacgcca
    20281 ggcgattgag catatcaaag cacacgttac ggcagaaacg ccaaagggca agcacgatat
    20341 cgaaggcaat cgtctgtttt atcttatctc ggaagatatg accgagccgt acgaagctcg
    20401 ccgtgcggag taccatgccc gctatcttga cattcagatt gtgttaaaag gtcaggaagg
    20461 catgaccttc agcacgcaac ctgcaggcgc gccggatacc gactggttag ctgataaaga
    20521 catcgcattt ttgccggaag gcgttgatga gaaaacagtt atcctaaatg aaggtgattt
    20581 tgttgtgttt tatccggggg aagtgcataa accgctgtgc gcagtgggcg cgccagccca
    20641 ggttcgcaaa gcagtagtga agatgctgat ggcgtgatga cttttcgccg taaataactc
    20701 caggtttacg gcgagtttgt gaaaagagcg ttttttgata tttttttgtg agtaaaattt
    20761 gtaatgctta gacgttctta ttaactcaag gagttcgtca tgagcaaaat atcagatttg
    20821 aattattctc aacacattac attagccgac aattttaaac aaaaaaatga agccttagat
    20881 acctggtatg tgggaatgaa tgattttgcc cgaattgccg gggggcagaa tagcagaagc
    20941 aatattcttt ctcctagagc atttttggag tttttggcaa agatatttac cctgggttat
    21001 gtggatttta gcaaacgctc caacgaagcg ggtagaaata tgatggctca tattgagttc
    21061 tcatcttatt ctaaagtact gatggcaatg aaaaaatgaa gttttacatg aataatcctg
    21121 aaggggagcg agcggattta tctaaggtga agacagaaat tacacttgcc tcagcttcta
    21181 ctaaagggat tcgtgaagga catacagtca ttatatttaa acaatctgat ggttcgacta
    21241 atcgttatga agggaagtct tttgaaagaa aagatgatag ttcattacac ctgattacta
    21301 acaaggttct ggcgtgttac caacgcgaag ctaacaagaa aatagcgcgt ctattaaata
    21361 atcatcagaa gttaaataat ctacagaagt taaataatat acaggagtta aatgattcgc
    21421 aggagttaaa taattcacag aagttaaata attcgcagga gttaaatgat tctcaggagt
    21481 taaataactc gcaggtgagt tgtaaaggtt cagttgattc tacgattacg gatttattag
    21541 aaaaagcatt gaataaagcc ttattagcaa taaggaacga acatctgcta ttaatgcctc
    21601 atgtatgtga tgaatagatt tcatacttac tgggcgaaaa tggtatactt gaagaaatag
    21661 ataagctcta cgaattaaat gatcacggaa ttgataatga caaagaaggt aacaatgaaa
    21721 ttaatgacat catgattaac ctgtctcata ttcttattga ttccttagat gatgcaaagg
    21781 ttaaccttac accggtcatc cattcgatgt tggtgacttt tttagaattg ccatataata
    21841 atgatgtaaa aatactggag tggtgtttta ataaaagcat gcaatatttt gatgattctg
    21901 caaagataga gcatgcatgc tccgtaataa atcatattaa ttttctctgc gatcagtcta
    21961 aagtagctga gacattattt ttcaatctcg ataaagaacc ctataaaaat agccctgaat
    22021 tacaggagtt gatttggaaa aagttggttg tatatgtcaa tgattttaac ttaagcaatc
    22081 gagaaaaaac atatttaata caaaggatat ttaataatgt tgagtcgcta tttaataaag
    22141 tacctgtcag cattttagtt aatgatattc ttctgaatga tttttttatg aaaaacactg
    22201 agatgattaa ctggtacttt cctcggttac ttaagagtta tgagggcgaa aaaaattatt
    22261 ttgataattt aaaatatgat ataaatgatg agaaatctaa taaagaaatt ttgaagaatc
    22321 aaccagataa tgttatcaaa gaaaaactga ataatgaatt caaacttaga tttagaatga
    22381 tgcaaactat ctttaaatcg aaggttaatg tatcgccata tattgaccaa cagcgtttaa
    22441 atacactaaa tcctccggaa aatttacgta tggcaataga aaaatttggc tggaagaaaa
    22501 aaactatcac tgcataaaat aatttgatgc cgggatgttt ttgtatttcc cggcatcttt
    22561 atagagatat taattattta atttactgag cgtcgcgacc atcaccgctt tgatggtatg
    22621 catgcggttt tccgcctgat caaatacaat gctggcggca gattcgaaga cctcatcagt
    22681 cacttccatt ccgccatgta ggccaaactc ttccgccatt tttttgccaa gcgtcgtttg
    22741 gtcgtcatga aacgcgggca ggcagtggag gaatttgacc tccgggttac cggtcaactg
    22801 catcatcttg ctgttcacct gataatcacg cagtaatgca atgcgttcag cccatttttc
    22861 ttttgcttca cccatcgaca cccatacatc ggtatagata aagtcagcac cttcaactcc
    22921 tttagctaca tcttccgtca gcgtaatatt cccgccattt tgttgtgcca gggcgcggca
    22981 ttccgtaacc agcgcagctt ctggccagca tgcctgtggc gcgaccagac gcaaatccag
    23041 accggtaagc gccgcagctt cgagcatcga attgcccatg ttgttacgtg cgtcacctgc
    23101 atagaccagc gtcatttcgt tgaatgcttt accgggtaaa tgctcctgca tggtgaggag
    23161 atccgccagc aactgcgtgg ggtgaaactc attggtcagg ccgttccata ccggcacgcc
    23221 agcgtattcc gccagtgttt cgacaatctc ctgaccatag ccgcgatact gaataccgtc
    23281 atacatgcga ccaagcacgc gggcagtgtc tttaatcgac tctttatgac caatctgact
    23341 accgcttggg ccaagataag taacgcgagc accctggtca tatgcggcaa cttcgaaaga
    23401 gcatcgggta cgagtcgagt ctttttcgaa gatgagcgcg atgtttttac cagtgagttt
    23461 ggcttcttct ttaccgcttt tcttatcagc tttcagcttc gcggctaact gcagcaggct
    23521 gttgagttca gctggcgtga aatcgagtaa tttcaggaaa tgcttatgat aaaacccgga
    23581 catagatccc tcctgtggct aacgcctcaa tgaattaaaa ttcaatttat atgtatgatt
    23641 attcatttgc aagtctaaag cataaatctt tgtcacaaag gtggaggcaa tgtcagtggt
    23701 gtgtgacaat aagagtatcg gcaggacatt aagaggaatg agccatggca aacccggaac
    23761 aactggaaga acagcgtgaa gaaacacgtt tgattattga agaattactg gaagatggca
    23821 gcgatccgga cgcgctgtac accatcgaac accatctttc cgcagacgat ctggaaactc
    23881 tggaaaaagc ggcagttgaa gcgtttaaac tcggttacga agtgaccgat ccagaagagc
    23941 tggaagttga agatggtgat atcgtgattt gctgcgacat cctcagcgag tgcgcgttga
    24001 atgccgatct gatcgatgcc caggttgaac aactgatgac gctggcggag aaatttgacg
    24061 ttgagtacga cggttggggc acttactttg aagatcctaa cggcgaagat ggcgacgatg
    24121 aagattttgt cgatgaagac gatgacgggg ttcgccacta attaattgat taaaatgccc
    24181 gatagcacat actagcgggc atttttttag agttcgcgta gcatccgcac ttcacaatcg
    24241 acatggcccg tgcagccaag cgcatagtcg atatgctcaa agcccaaatg ctcataaagc
    24301 gcaatggctt cctttaaaaa agcggtcgtt tccagatagc agcgtttgaa gcccatctct
    24361 cgtgcctgct ccatcgccat taaggccagt tttttcgcta gccctttacc gcgaatagcg
    24421 ggaagaaagt acattttctg cagttcgcaa atgtccgctt cactacctgt taatggcgca
    24481 atcccgccgc cgccgaccac ttcgccattg tattcaacca cccaataagc atggccaggc
    24541 tggctgtaca cttgatacag ttcgtcgaga ttcggatcgg caacggtgta gcctttatcg
    24601 gcggtaagac catactctgc gtaaacctga cggatgacgc tggcgatagc aggattatcc
    24661 tgcagcgtca ggcgacgcat tacaggtgat tgcggcgcaa tgttattcat agtttaaccc
    24721 aataaaaata aagtaattat acatttgttt aataccactc tcttcttacg tgcgcaagcg
    24781 tcttataaag caataaagtt tttgccagcc tattactgat aagagtattg taacgtggca
    24841 gaacatttta ctgtctgtat ctataaatta tatcaatgga ttttttaatt caggggattt
    24901 ttatggctca agttattaat gaaatggatg ttccgtccca ttcttttgtt tttcatggta
    24961 caggtgagag atattttctt atttgtgtgg tgaatgtgtt gttaacgatt ataacgctag
    25021 gtatctattt accatgggca ttaatgaaat gtaagcgtta tctttatgct aatatggaag
    25081 ttaacggaca acgattttct tatggaatta ccggtgggaa tgtttttgtt agttgtcttg
    25141 tttttgtttt tttctatttc gcaatcttaa tgacagtgtc agcagatatg ccgcttgttg
    25201 gctgtgtttt gactttgtta ctgttggttt tgcttatatt tatggcagca aaaggactgc
    25261 gttatcaggc cttaatgacc agtctcaacg gcgtaagatt tagttttaat tgctctttga
    25321 aagggttctg gtgggtaacc tttttcttgc cgattttaat ggccattggg atggggactg
    25381 ttttctttat ctcgacaaag atgctacatg ccaatagttc aagtagtgtt attatatctg
    25441 tggttctgat ggcaatagtt ggtattgttt ccattggtat ttttaatggt actttatata
    25501 gcctggtaat gagttttctc tggagcaata ccagtttcgg tatacatcgt ttcaaggtga
    25561 aattagatac tgcgtattgt ataaaatatg ccatcctcgc atttttagct ttattacctt
    25621 ttctcgctgt tgctggttat attatcttcg atcaaatatt aaatgagtat gatagttctg
    25681 ggtatgcaaa tgatgatatt gagaatttac agcaatttat ggaaatgaaa cgtaaaatga
    25741 taatcgcgca gttaatttat tattttggga ttgctgttag caccagttat ttaacggtgt
    25801 cgttgcgaaa tcattttatg agcaacctgt cattgaatga tgggcgtatt cgttttcgct
    25861 caactttaac gtaccacggt atgctttatc gcatgtgtgc gttggtggtg atatccggga
    25921 ttacgggtgg tctggcttac ccactgctga aaatatggat gattgactgg caggcaaaaa
    25981 atacgtattt gctgggcgat ttggatgacc ttcctttaat caataaagaa gaacaaccag
    26041 ataaaggctt cttagccagt atttctcggg gagttatgcc ttctttacca tttctgtaag
    26101 agataaaaaa ggccggagca tgctccggcc ttcgttttca tcactgtgtt gattacagtg
    26161 cggcgataac agcctgctgt tcaatcagct tggctttcgc ttctgcataa ccttccagct
    26221 tctcacgctc tttcgcgata accgcttccg gtgcgcgggc gacaaagcct tcatttgcca
    26281 gcttgttctc gatgcggctg atttcacctt cgatcttcgc cacttctttc gccaggcgcg
    26341 ccagctcatc ttctttgttg atgaggccag ccatcgggat cagcagctct gcgccgtcga
    26401 tgatcttggt aacggaaacc ggacctttgt catcggcagg cagcacggtg atgctttcca
    26461 gacgcgccag cgtttgcagg aagccacggt tttcatttac gcgacgttct gcatccgcgc
    26521 tgcaaccacg cagcagcagc tccagcggtt tgcccggcgc gatgttcatt tctgcacgga
    26581 tgttacgtac agcaacgatc gcctgtttca gccactcggt gtcggccagt gcggcttcat
    26641 caacctgaga tgcatcatac tgcgggaacg gctgcagcat gatggtgtcg gcagtgatac
    26701 cgcaaagtac tttcacacgc tgccagatgg tttcggtgat gaacggaatg atcggatgcg
    26761 cgaggcgcag cagaccttcc agtacagtca ccagcgtatg gcgagtaccg cgcagttcgg
    26821 cttctgttcc accgttcatt accggcttgg tcagctccag ataccagtcg cagaactggt
    26881 tccaggtgaa ctcatacaaa atgcctgcgg cgatatcgaa gcggaagctg tccagcgctt
    26941 cgcggtacgc tttgatagtc tggttgaact ccgccagaat ccagcggtcc gccagcgata
    27001 gcgtcatttc gccgccgttg aagccgcaat cctgaccttc tgtgttcatc agcacaaagc
    27061 ggctggcgtt ccacagcttg ttacagaagt tacggtagcc ttccagacgc ttcatatccc
    27121 agttgatgtc acgaccggta gacgccagcg ccgccagggt gaagcgcagg gcatcggtgc
    27181 cgtgcggctc aatgccgttc gggaactgtt tctcggtgcg cttacggatt ttgtccgcca
    27241 gctgtggctg catcatattg ccggtacgtt tttccagcag ttctggcagc gaaataccgt
    27301 caaccatatc cagcgggtcg ataacgttac ccttggattt ggacatcttc tgaccttcgt
    27361 cgtcacgaat cagaccggtc atgtaaacag tgtggaacgg cacctgcggt ttgccatttt
    27421 catctttgat gaagtgcatg gtcatcatga tcatgcgggc aatccagaag aagatgatgt
    27481 cgaaaccaga taccatcacg ctggttgggt ggaactgacg cagggcgtcg gtattttccg
    27541 gccagccaag ggtagagaag gtccacagtg cagaagagaa ccaggtgtcg agaacgtctt
    27601 cgtcctgacg cagggcaacg ttggcaccga ggttattttc cttgcgcact tcttcttcgt
    27661 tgcggccaac ataaacatta cccgcttcgt cataccatgc cgggatacgg tgaccccacc
    27721 acagctgacg agagatacac cagtcctgaa tatcgcgcat ccaggagaag tacatgtttt
    27781 cgtactgctt cggtacgaac tgaatgtcgc cgttctcaac cgcttcaact gccggtttcg
    27841 ccagtacatc ggcacgcacg taccactggt cggtcagcat cggttcgata accacgccgc
    27901 cacggtcgcc gtaaggaacg gtcaggtcgt gcggtttaat ttcttccagc aggccgagtg
    27961 catcaaccgc agcaacgact gctttacgtg cagcaaaacg ctcgagtttc tggaactctg
    28021 ccgggatctc gctggaataa acgtcagatt cgttaccttt ggtatcgaac acctgggcgc
    28081 tttcacggat atcaccgtca aaggtcagga tgttgatcat cggcagggcg tgacgtttac
    28141 cgacttcata gtcgttaaag tcgtgcgccg gggtgatctt cacgcagccg gtgccttttt
    28201 ccatgtcggc gtgttcgtcg ccaacgatcg gaatacgacg gttaaccagc ggcagaatga
    28261 catatttgcc aatcagatct ttgtaacgcg gatcttccgg gttaacagct acaccggtat
    28321 cgcccagcag ggtttccggg cgagtggtcg caaccaccag ataatcttta ccgtctgcgg
    28381 ttttcgcgcc gtcagccagc ggatagcgga tgtgccacat cgaacctttc gattcgtggt
    28441 tttccacttc caggtcagaa atagcggtac gcagtttcgg gtcccagttt accaggcgct
    28501 tgccacggta aatcaggtct tctttataca gacgaacgaa aacttctttc accgcattgg
    28561 acaggccttc gtccatggtg aagcgttcac gctcccagtc gacggagttg ccgagacggc
    28621 gcatctgacg ggtaatggtg ccgccagatt ccgctttcca ttcccagatt ttgtcgatga
    28681 aagcttcgcg gccgtagtcg tgacgggttt taccttcttc tgcggcaatc ttgcgctcaa
    28741 cgaccatctg ggtagcgatc ccggcgtggt cagtaccgac ctgccacagg gtgtttttgc
    28801 cctgcatgcg ctgatagcgg atcatggtat ccatgatggt ttgctggaag gcgtgaccca
    28861 tatgcaaact gccggtgacg ttcggcggcg ggatcatgat gcagaaactt tcctggcttt
    28921 catcgccatt aggcttaaag tagccctgct tttcccagtg ctcgtaaagc ggctgttcga
    28981 tatcttgtgg gttgtatgtc ttttccatta tttccaggtt gccgtattca ggttgaaacc
    29041 agccacgcgg taggctttat agcgttcgcg cgccagttgt ttcagagaat cttcataagg
    29101 aacgaagtct accacttctg tgaaagcggt ggcaaaatct gcaaagcttg ttcgcagact
    29161 aatcaatata tcgcgcgggc tgctgctgcg tttttgcggc caggcgatct ccaccggcgc
    29221 accgccgcgc ggtccttctc ccgctaaatt atgcggaaca aagctttctg ccggacgcgc
    29281 ccacagggct tcatccagcc ggtaagcctg cttttcatct tcacaggcga tgagtacgcg
    29341 cttaccgctg cgccaacgtt ctgcggcaat ttcacacacc agttgctcaa cggcgcttaa
    29401 gccatcgacg gtggtgtcat tgtccagaag gtagaacgtt gcgtttttca tatatggggc
    29461 ttcttgtggt ggatttaaat attgctgcat tcctcaccct gaaagatgag caatgaattt
    29521 ctattgcgct cgatcggttc cctctccctt agggagaggg ttagggtgag ggtaaaacgc
    29581 aaacttactc ttcgccgtta aaccgagcgc ggtttaacag gaactgtgcc agcaacgcta
    29641 ccggacgacc ggttgcgcct tttgctttac cagaacgcca ggcggttcct gcaatatcca
    29701 ggtgcgccca gttatactta cgggtaaagc gtgacaggaa gcaacctgcg gtaatcgccc
    29761 caccaggacg accgccaatg ttcgccatat cggcaaaatt ggactccagc tgttcctgat
    29821 actcgtcacc cagcggtaag cgccatgcgc ggtcaccgga ttgttcagac gcggcaatca
    29881 gttcatgggc cagcggatta tggttcgcca tcagaccagt aatatgatga cccagcgcga
    29941 tcacgcaggc accggtcagc gtcgccacgt caatcactgc ttccggctca aaacgttcaa
    30001 cgtaagttaa cacgtcgcac agtaccaggc ggccttcggc atcggtgttc agcacttcaa
    30061 cggtttgacc ggacatggtg gttaacacat cgcccggacg ataggcacgc ccgccaggca
    30121 tgttttcaca gcctgccagc acgccaataa cgttaatcgg cagttgcagc tccgcgacca
    30181 tacgcatcac gccgtaaacc gccgccgcgc cgcacatatc gtacttcatc tcatccatgc
    30241 cttctgaagg cttgatggag ataccgccgg agtcgaaggt taaaccttta cccaccagca
    30301 cgattgggcg tgcatcttcc gacgcgttgc ctttgtactc aatcaccgac atcagcgatt
    30361 cgttttgtga accctgaccg accgccagat aagagtgcat tcccagctct ttcatctgct
    30421 gttcgccgat aacgcgggtg atgacattct tgctgtagct gtcagccagc tggcgcgcct
    30481 gagaggcgag ataagcggcg ttacagatat tcggcggcat attaccgaga tctttcgctg
    30541 cttttatccc ggcggcaatc gccagaccgt gctggatcgc gcgctcaccg ctggtcagtt
    30601 cacggcgggt cggcacgttg aacaccattt tacgcagcgg acgacgcggt tcgctcttat
    30661 tcgttttcaa ctgatcgaaa ctgtagagcg tctcttttgc tgtctcgaca gcctgacgca
    30721 ctttccagta gttgttacgg cctttaacgt gcagttcagt cagaaagcag accgcttcca
    30781 tcgagccagt atcattcagc gtattaatgg ttttctgaat aacctgcttg tactgacgct
    30841 catccagctc acgttctttg ccgcaaccaa taaggagaat tcgctcggaa agtacattcg
    30901 gaacatggtg cagcaacaat gtctgccccg gttttccttc cagttcgccc cggcgtagca
    30961 gggcgctgat gtacccatcg ctgattttat cgagctgttc tgcaatcgga gaaaggcgac
    31021 gtggttcgaa gacgcccacg acgatgcagg cactccgctg tttctccggg ctaccgcttt
    31081 ttacactaaa ctccatgcac tacgctcctg aatcttaaag acaacggcgg tggctacaga
    31141 tagaattgca agctttcgta actcatgtcc gctgttgcga tgacttcgtg ttaatcttaa
    31201 cgttattacg gcattggcac gtcagaacaa aatctgagag gtgaatccgt tgagtataat
    31261 tatcttagcg acgatttcga cgactcaaga gaataaatga cgtttaagcc atgaaacaag
    31321 ctaaaatcct gcaaaagacg agtttttacg ggcgtattta aagtgataat cataagatat
    31381 ctggtgcggg agacgctcaa aagccagctg gcgatactct tcatcttgct tttgatcttc
    31441 ttctgtcaaa agttagtgag gatcctcggc gcagcggttg acggcgatat tccggcgaat
    31501 ctggtgctct cccttctcgg gttgggcgtg ccggaaatgg cgcagcttat cctgccatta
    31561 agcctgttcc tcgggctgct gatgacgctg ggcaaactgt ataccgaaag tgaaattacg
    31621 gtaatgcatg cctgcggctt gagcaaagcg gttctggtga aagcggcaat gatccttgcg
    31681 gtattcacgg caatcgtcgc ggcggttaac gtgatgtggg cgggaccgtg gtcatcgcgt
    31741 catcaggatg aagtgttagc agaagcgaaa gcgaaccctg gcatggcggc gctggcgcaa
    31801 gggcaattcc agcaagcgac taatggcagc tcggtgctgt tcatcgaaag cgttgacggc
    31861 agcgatttca aagatgtgtt cctcgcacaa attcgaccaa aaggtaatgc acgtccttct
    31921 gtggtggtgg ccgattccgg acatttaacc cagctgcgcg acggctccca ggtcgtcact
    31981 ctcaaccagg gaacgcgctt cgaaggcact gcactgttac gtgatttccg cattacggat
    32041 ttccaggatt atcaggcgat cattggtcac caggcggtgg cgctcgaccc gaacgatacc
    32101 gaccagatgg acatgcgcac attgtggaac actgacaccg atcgtgctcg cgcagaactg
    32161 aactggcgta tcacgttggt attcaccgtg tttatgatgg cacttatggt tgtaccgttg
    32221 agcgtggtta acccgcgtca gggacgcgta ctgtcgatgc tgccagccat gctgctgtat
    32281 ctacttttct tcctgatcca gacctccctg aaatcgaacg gcggtaaagg taagctggac
    32341 ccgacactgt ggatgtggac cgttaacctg atttatctgg ctttggcgat tgttctcaac
    32401 ctttgggaca ccgtgccggt ccgccgcctg cgcgccagtt tttcgcgtaa aggagcggtg
    32461 tgatgcaacc ttttggcgta cttgaccgct atatcggtaa aactattttc accaccatca
    32521 tgatgacgct gttcatgctg gtgtcgctgt cgggcattat caaatttgtc gatcagctga
    32581 aaaaagccgg gcaggggagt tacgacgcgt taggcgcagg aatgtatacc ttgctgagcg
    32641 tgccgaaaga tgtgcagatc ttcttcccga tggcggctct tcttggggcg ttgcttggtc
    32701 ttgggatgct ggcgcagcgc agcgaactgg tggtgatgca ggcttctggt tttacccgta
    32761 tgcaggtagc gctgtcggtg atgaaaaccg ccattccact ggtcttgctg acgatggcga
    32821 ttggcgaatg ggtcgcgccg cagggcgaac agatggcgcg taactaccgt gcgcaggcga
    32881 tgtacggcgg ctcgttgctc tctacccagc aaggtttatg ggcgaaagat ggcaacaact
    32941 tcgtctacat tgagcgggtt aaaggtgacg aggagttagg tggcatcagt atttatgcct
    33001 ttaacgagaa tcgtcgtctg caatccgtac gttatgccgc taccgcgaag tttgacccgg
    33061 aacataaagt ctggcgtctg tcgcaggttg atgaatctga tctgaccaat ccgaaacaga
    33121 ttaccggttc gcagacggtg agcggcacct ggaaaaccga cctcacgcca gacaaactgg
    33181 gcgtggtggc gctggacccg gatgcactct ccatcagtgg tttgcacaac tacgtgaagt
    33241 atctgaagtc gagcggtcag gatgccggac gttatcagct caacatgtgg agcaaaatct
    33301 tccagccgct atccgtggcg gtgatgatgc tgatggcgct gtcgtttatc tttggcccac
    33361 tgcgtagcgt accgatgggc gtgcgtgtgg tcacaggtat cagcttcggt tttgtcttct
    33421 acgtactgga ccagatcttc ggcccgctga cgttggttta cggcatcccg ccgatcatcg
    33481 gcgcactgtt gccaagtgcc agcttcttct taatcagcct gtggctgtta atgagaaaat
    33541 cgtaatctaa caagggagcg aaagctccct ttattattgt tgacaaagtc aaatacgagt
    33601 tcgaacggtc ccctcgcccc tttggggaga gggttagggt gaggggcaca gatcacgtct
    33661 gacttatctt tttctccccc ccagcaaact ccccaacatc ccgcgtacaa tctgattcgt
    33721 cacctggcga gcggcgcttt tcgccattgt ttgcaccaca ccatctttct tcccgccgcg
    33781 tggtccggta gtgccaaaca aaatatcctt caatccacca agaataccgt catccaccgc
    33841 cacctctttc cctttcacgg ggggattatt ttgctgctcg gtactggcct gaaagccttt
    33901 ttgcagcatc tcataggcgg actcgcggtc cacctcatct tcatatttgc catacaccgg
    33961 ggagtgatta atcaggccat tacgctcatc ttccgtcacc ggccccattc gcgaacaagg
    34021 tgcgatcacc atcgcccgtt ccaccacaga aggacttcct tttgcatcga gaaacgagat
    34081 taacgcctcg ccggtcccca gttcctggat tgccttttcg gtatcaaatg ccggattggc
    34141 ccgcatggtt tgcgccgctg tcttcactgc tttctgatct tttggcgtaa aagcccgcaa
    34201 agcgtgctga acacgattac cgagctggcc gagcacatta tccggaatat cagacgggtt
    34261 ttgcgaaacg aaccagacgc ccacgccttt tgagcgaata agccttatca cctgctcaat
    34321 cttatccagc agtacctgcg gtgcatcgtt aaacagcaga tgtgcctcgt cgaagaaaaa
    34381 caccagtttt ggtttctcca gatcgcccgc ttccggcaat tgttcataca actctgaaag
    34441 catccacagc aggctggcgg cgtacagttt cggcatctga taaagtttct cggcgctgag
    34501 gatattgata acgcctttac cgttggcatc ggtgcgcatc cagtctttga tatccagcat
    34561 cggctcacca aagaagtgtg ctgcgccttg ctgttccagc gacagtaatc cgcgctggat
    34621 ggcaccaacc gatgcgctac tgatatttcc gtactgattc tggaaggatt tggcgttatc
    34681 gccgatgtac tgggtaatcg cccgcaaatc tttaaagtcg agcagcaaca atccctgatc
    34741 gtccgcaatg cggaagataa tattcagcac gccagattgc acatcgttga gattcaacag
    34801 tcgcgccagc aacagcggcc ccagatccga aaccgtcgcc cgcaccgtgt ggcctttctc
    34861 gccaaagata tcccacacca ccaccggatt ggcatgcggt tgccagtcat tgacgccgat
    34921 atttttaagc cttgtgagca gtttttccga cgccgttcct gcctgcgcga taccggtcag
    34981 atcgcctttc acatcagcca taaacaccgg cacgccgatt tccgacaatg actctgccag
    35041 tttttgcagc gtaacggttt tacccgtccc cgttgcgcca gtaatcagcc cgtgacggtt
    35101 agccattccc ggcagtaaaa acagttctgt gtccggcgtg cgggcaatta acaggggttc
    35161 gctcatgaga tttcctccag tttcctgcct ggagtatagg caacggctgt gcactcttca
    35221 ccgctaaatt cagcagattg cgagacgcct tccaaagaga tttaagtgcc gggcgacagc
    35281 acccggcaaa gggatcagaa gtcggctttc aggactactc ggtagcgcgc tttaccatcg
    35341 cgtacatgct gaatggcgtc gttaattttc gacatcggga acagttcggt tgtcggcgca
    35401 accttgctgc gggcggcaaa gcgcatcagc tttcgcagct cataaggcgt gccggtagca
    35461 gaaccagaga tgctgcggtc gcccgcaatt aacgtaaagg ccggaacaga cagcggcgtg
    35521 agaaccgcac cgaccgtatg gaaattaccg ccataggtca gcgcctcaaa ataaggctgc
    35581 cagtcgaggc tgacgttcac agtattgata atgagatcaa actgccccga cagggctttc
    35641 agtgcctgcg gatcgcggct attcaccact ttatcggcac ccatcgccag tacttcctgc
    35701 tctttcgccg gattagaact aaaggccgtc acctcgcatc ccattgcgtg cagaagtttt
    35761 atagcgatat gccccagccc gccaatacca attaccccaa cgcggctggt agcagtgata
    35821 tggtgcatca acagtggttt aaagaccgtg ataccgccgc ataacagcgg cccggcggac
    35881 tcgatatcaa tattttctgg cagtggaatc acccattgcc agtccgcacg caacttctcg
    35941 gcaaagccac cgcgattcat aatcgtcggc accgcacctt gctcgcagtt gatctgatta
    36001 ccgctaatac aggcgtcgca gtgaccacag ctacgcgccg tccagccaat cccgacacgc
    36061 tgaccgacct gcaaaccttt atcctgcgcg gcgctcccga gtgccaccac gcgcccaatc
    36121 acctcatgcc cggcaaccag cggatattgt gaaaatcccc attcgttatc gatcatcgac
    36181 agatcggaat gacaaatccc gcagtaatcc acctgcactt caacatcttg tggcttcagc
    36241 tcaccgggat cgtactcata aacttccagt tcgccgcccg cttcttttgc ggcatagctt
    36301 tttatcatcg acatttttgg gtccttctct ggtgttgttt gggaagtgta gagcatggca
    36361 gggcggggtg cctggagtgt gacaaaggtt acatatcgct gagtgtaatg ctgaaaattt
    36421 cagcacttag cgaggtgggt gcaagctggc gcttgcatgg tggcgtgcga caggtataat
    36481 ccacaacgtt ttccgcatac ctcttcagtg ccgaagtggc gaaatcggta gacgcagttg
    36541 attcaaaatc aaccgtagaa atacgtgccg gttcgagtcc ggccttcggc accatcggaa
    36601 catcaataga cgtcaacgga cgtctttttt tgtgcctaaa atccagtatc cgcaaggctt
    36661 tcctcgcttt ttcactcaac ctaagtcaac ctgattcaat ctacatcaac ttactgatgc
    36721 gggtacaact gcgggtattt cccggttcga taatgtttgt acccacacag accccttgaa
    36781 aggatactca tcatggctct gagtgatgtg aaggttcgtt cggctaagcc tgaagcaaaa
    36841 gcctataagc tgaccgacgg tgaaggcatg gttttgctgg ttcaccctaa cggctccaaa
    36901 tactggcggc tacgttatcg ctttggcggc aaagagaaga tgctggcatt ggggaaatac
    36961 cccgaagtgt cgttggcgga tgccagggca cgccgggatg aggctcgtaa gcttttagct
    37021 aatggtgtcg atcccagtga aaacaagaaa gctgttaagg tagaacagga acaagaggcg
    37081 ataacgtttg aagtagtcgc cagagactgg cacgccagca atcagaagtg gtcggcatcg
    37141 catagcgctc gtgttttgaa aagtctggag gataatctct ttactgctat cggtaagcgg
    37201 aacattgcgg aactgaagac gcgggatctt cttgtaccca ttaaagcagt cgagtcatcc
    37261 gggcggctcg aagttgccgc ccgtttacag cagcgtacta ccgcgattat gcgctttgcc
    37321 gttcagagcg gcttaatcga ctacaacccc gcgcaagaga ttgcaggtgc ggttgctacg
    37381 gcgaaaagac agcaccgtgc agctcttgaa ctgaaccgta tacctgaatt acttcatcgc
    37441 atagatcact attctggcag accgttaact cgactggcgg ttgaactcac tttattggtt
    37501 ttcatccgtt cgagcgagct gcgttttgcc cgctggtcag aagtagattt tgaaacggct
    37561 atgtggacaa tcccaggtga gcgtgagcaa ctggaaggag tcaagcattc tcagcgtggc
    37621 tcgaagatgc ggacaccaca ccttgtacct ttgtcgcggc aggccctaag tattttggaa
    37681 aagatcaaaa gcatgagcgg aaatcgagag ctgatttttg tgggcgatca cgatccacgt
    37741 aagccgatga gtgagaatac ggtgaacaag gctttgcgcg taatggggta tgacacgaag
    37801 gtcgaggttt gtgggcatgg cttcaggacg atggcttgta gttcgttgat tgagtcggga
    37861 ttatggtcga gggatgcggt agagcggcag atgagtcacc aggagcgtag ctctgtgcga
    37921 gcagcttaca tccataaggc ggaacatctt ggggagcgta ggttgatgtt tgaagtggca
    37981 cactgaattt ggccacctga acagaggtga tatgctcacc tca
//
LOCUS       DADLSB010000021        37171 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.021, whole genome shotgun sequence.
ACCESSION   DADLSB010000021 DADLSB010000000
VERSION     DADLSB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.021"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..790
                     /locus_tag="JN331_001065"
     CDS             <1..790
                     /locus_tag="JN331_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7259265.1"
                     /translation="EHADKTGREAVMFSSFLRAQVSLAWVIGPPLAYALAMGFSFTVM
                     YLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDTLLLFVICTLMWGSNS
                     LYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFAKRLGKRFLMRVAAVG
                     GVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLMPGQAGSATTLYTNTS
                     RVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKDV"
     gene            complement(787..1041)
                     /locus_tag="JN331_001066"
     CDS             complement(787..1041)
                     /locus_tag="JN331_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY7259266.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            1196..1768
                     /gene="yeiP"
                     /locus_tag="JN331_001067"
     CDS             1196..1768
                     /gene="yeiP"
                     /locus_tag="JN331_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY7259267.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1991..3457
                     /locus_tag="JN331_001068"
                     /pseudo
     CDS             1991..3457
                     /locus_tag="JN331_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            3575..4561
                     /locus_tag="JN331_001069"
     CDS             3575..4561
                     /locus_tag="JN331_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY7259268.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            4600..5313
                     /gene="lpxT"
                     /locus_tag="JN331_001070"
     CDS             4600..5313
                     /gene="lpxT"
                     /locus_tag="JN331_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY7259269.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALTIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            5725..6291
                     /gene="mepS"
                     /locus_tag="JN331_001071"
     CDS             5725..6291
                     /gene="mepS"
                     /locus_tag="JN331_001071"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY7259270.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            6472..8028
                     /locus_tag="JN331_001072"
     CDS             6472..8028
                     /locus_tag="JN331_001072"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7259271.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            8110..9924
                     /locus_tag="JN331_001073"
     CDS             8110..9924
                     /locus_tag="JN331_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7259272.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDCLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9925..11019
                     /locus_tag="JN331_001074"
     CDS             9925..11019
                     /locus_tag="JN331_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY7259273.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     DNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            11019..12044
                     /locus_tag="JN331_001075"
     CDS             11019..12044
                     /locus_tag="JN331_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7259274.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            12046..13635
                     /gene="yejF"
                     /locus_tag="JN331_001076"
     CDS             12046..13635
                     /gene="yejF"
                     /locus_tag="JN331_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY7259275.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(13639..13983)
                     /locus_tag="JN331_001077"
     CDS             complement(13639..13983)
                     /locus_tag="JN331_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259276.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(14316..15506)
                     /gene="bcr"
                     /locus_tag="JN331_001078"
     CDS             complement(14316..15506)
                     /gene="bcr"
                     /locus_tag="JN331_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY7259277.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(15534..16229)
                     /gene="rsuA"
                     /locus_tag="JN331_001079"
     CDS             complement(15534..16229)
                     /gene="rsuA"
                     /locus_tag="JN331_001079"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY7259278.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            16379..18139
                     /locus_tag="JN331_001080"
     CDS             16379..18139
                     /locus_tag="JN331_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY7259279.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKENHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            18264..18548
                     /gene="rplY"
                     /locus_tag="JN331_001081"
     CDS             18264..18548
                     /gene="rplY"
                     /locus_tag="JN331_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY7259280.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(18687..19694)
                     /gene="yejK"
                     /locus_tag="JN331_001082"
     CDS             complement(18687..19694)
                     /gene="yejK"
                     /locus_tag="JN331_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY7259281.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19876..20103
                     /locus_tag="JN331_001083"
     CDS             19876..20103
                     /locus_tag="JN331_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY7259282.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            20123..21883
                     /gene="pbgA"
                     /locus_tag="JN331_001084"
     CDS             20123..21883
                     /gene="pbgA"
                     /locus_tag="JN331_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY7259283.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21958..22034
                     /locus_tag="JN331_001085"
     tRNA            21958..22034
                     /locus_tag="JN331_001085"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21992..21994,aa:Pro,seq:ggg)
     gene            complement(22137..24728)
                     /gene="yejO"
                     /locus_tag="JN331_001086"
     CDS             complement(22137..24728)
                     /gene="yejO"
                     /locus_tag="JN331_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY7259284.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYDETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGNITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGNMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNLVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            25048..25695
                     /gene="narP"
                     /locus_tag="JN331_001087"
     CDS             25048..25695
                     /gene="narP"
                     /locus_tag="JN331_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY7259285.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     ILHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25906..26958)
                     /locus_tag="JN331_001088"
     CDS             complement(25906..26958)
                     /locus_tag="JN331_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY7259286.1"
                     /translation="MRFLLGVLMLMIFGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26955..27512)
                     /gene="dsbE"
                     /locus_tag="JN331_001089"
     CDS             complement(26955..27512)
                     /gene="dsbE"
                     /locus_tag="JN331_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY7259287.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(27509..29452)
                     /locus_tag="JN331_001090"
     CDS             complement(27509..29452)
                     /locus_tag="JN331_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7259288.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFTIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(29449..29928)
                     /gene="ccmE"
                     /locus_tag="JN331_001091"
     CDS             complement(29449..29928)
                     /gene="ccmE"
                     /locus_tag="JN331_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY7259289.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29925..30134)
                     /gene="ccmD"
                     /locus_tag="JN331_001092"
     CDS             complement(29925..30134)
                     /gene="ccmD"
                     /locus_tag="JN331_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY7259290.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(30131..30868)
                     /gene="ccmC"
                     /locus_tag="JN331_001093"
     CDS             complement(30131..30868)
                     /gene="ccmC"
                     /locus_tag="JN331_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY7259291.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGLA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30910..31572)
                     /gene="ccmB"
                     /locus_tag="JN331_001094"
     CDS             complement(30910..31572)
                     /gene="ccmB"
                     /locus_tag="JN331_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY7259292.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(31569..32186)
                     /gene="ccmA"
                     /locus_tag="JN331_001095"
     CDS             complement(31569..32186)
                     /gene="ccmA"
                     /locus_tag="JN331_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY7259293.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(32205..32807)
                     /gene="napC"
                     /locus_tag="JN331_001096"
     CDS             complement(32205..32807)
                     /gene="napC"
                     /locus_tag="JN331_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY7259294.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32817..33266)
                     /gene="napB"
                     /locus_tag="JN331_001097"
     CDS             complement(32817..33266)
                     /gene="napB"
                     /locus_tag="JN331_001097"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY7259295.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(33263..34126)
                     /gene="napH"
                     /locus_tag="JN331_001098"
     CDS             complement(33263..34126)
                     /gene="napH"
                     /locus_tag="JN331_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY7259296.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(34113..34808)
                     /gene="napG"
                     /locus_tag="JN331_001099"
     CDS             complement(34113..34808)
                     /gene="napG"
                     /locus_tag="JN331_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY7259297.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34815..>37171)
                     /gene="napA"
                     /locus_tag="JN331_001100"
     CDS             complement(34815..>37171)
                     /gene="napA"
                     /locus_tag="JN331_001100"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY7259298.1"
                     /translation="PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLP
                     KIMYGKDRLTQPLLRMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMF
                     GSGQWTIWEGYAASKLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIE
                     QADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQ
                     SDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDA
                     SEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHT
                     RGVWANNLVYNLHLLTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNE
                     KHRDICEKKWNIPSGTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEER
                     MPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAP
                     GEAKSDLWQLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELA
                     EDQLNDESRELGFYLQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQW
                     RYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWH
                     TGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNR
                     PPQGLVYMPFFDAAQLVNKLTLDATDPLSKETDFKKCAVKLEKV"
BASE COUNT         9012 a        10041 c         9210 g         8908 t
ORIGIN      
        1 tgaacatgcc gacaaaaccg gacgtgaggc ggtgatgttc agctcttttt tacgcgctca
       61 ggtttcactg gcatgggtca ttggcccacc gctggcttat gcgttagcga tgggtttcag
      121 ctttacggta atgtatctga gcgcagcggt agcgtttatt gtttgcggcg tgatggtgtg
      181 gctgttttta ccgtcgatgc aaaaagagct tccgctggcg accggcacgg ttgaagcgcc
      241 gcgccgtaac cgtcgcgata cgctactgct gtttgtcatt tgtacattga tgtggggctc
      301 gaacagcctg tacatcatca acatgccgct atttattatc aacgaactgc atcttcccga
      361 gaaactggcc ggtgtgatga tggggaccgc cgccgggctg gaaatcccga cgatgttgat
      421 tgccggatat ttcgccaaac gtctgggtaa gcgtttctta atgcgcgttg ctgccgtggg
      481 tggcgtctgt ttttacgcag gaatgctgat ggcgcattca cctgccattc tgttgggctt
      541 gcagctgcta aatgctattt ttattggcat tctgggcggc atcgggatgc tctattttca
      601 ggatttgatg cccggtcagg caggttcagc caccacgctc tataccaaca cgtcgcgcgt
      661 gggctggatc atcgcaggat cagtggcggg catcgtcgcc gagatctgga attatcacgc
      721 tgtgttctgg tttgcgatgg tgatgattat cgccactctg ttttgcttac tgcggattaa
      781 agatgtttaa ggcgcggtca gcatttccag atcgataagc caggtcatgg cctgctgacg
      841 cgagttaccg cacatttcag cgctggcttg taagcctgca cagactttcg gtcgcagtgg
      901 cgaggtgaaa attttacagc gttgctgttc atcaagctga atgcagggcg tattggcggg
      961 cttgccatct ggcataccgg gaatagggct ggaaattgaa ggggcagtgc aacacgcccc
     1021 gcaacccgga cggcattcca tacgttgttc tcttaaggtt aaccaccgga ttgatgagcg
     1081 cgcacagtac cacctttttt gcaccagcaa aagtgtgaat accacttgcc cgaaaggccc
     1141 gtcgcgagta ctttgtcgcg atatttttga cattttcgac tacaggaatt tttcgatgcc
     1201 aagagcgaac gaaattaaaa aaggtatggt actgaattac aacggcaaac tgctgctggc
     1261 taaggatatt gatattcagt cgcccactgc ccgcggtgcc gctacgctgt acaaaatgcg
     1321 tttttctgat gtccgtaccg ggctgaaagt agaagagcgt ttcaaaggtg atgatatcgt
     1381 tgacaccgtg acgctgaccc gccgttacgt tgacttctcc tatgtcgatg gcaacgaata
     1441 tgtctttatg gataaagaag actatacccc gtataccttc accaaagatc agattgaaga
     1501 agagttgctg tttatgccag agggcggcat gccggacatg caggtgctga cctgggacgg
     1561 tcaactgctg gcgcttgagc tgccgcagac cgttgatctg gaaatcgttg aaaccgcacc
     1621 aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg gcgacattga gcactggtct
     1681 ggtgattcag gtaccagaat acttaagccc gggcgaaaaa attcgtatcc atatcgaaga
     1741 acgccgttat atggggcgtg ctgactaact tcagccgcat gcagaaaagg gatagctcag
     1801 gctgtccctt ttttaattta ttataccaat cttctatttt gcgcttcctg aacaccttac
     1861 gccctcaatt ttcactcgtt gattgatcgc cctcacactt catcgcatta acaatccaga
     1921 ccaatttcaa ttgctgtcat ataactttac actgtcattg ttaatttatc gttactaaga
     1981 cgtgactcct atgaagacaa ttgcctccgt tacgctcccg catcatgtac acgctccacg
     2041 ctacgatcgc cagcagttgc aatcacgtat cgttcatttt ggctttggcg cctttcaccg
     2101 cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc cagggcggcg actgggggat
     2161 ctgtgaaatc agcttgttca gcggtgatca actgatgagc cagctccgcg cacagaacca
     2221 tttatatacc gtgctggaga aaggtgcgga cggcaatcag gtgataattg tcggtgccgt
     2281 tcacgaatgc cttaatgcaa aactggattc cttagcggca attattgaga aattttgcga
     2341 gccacaggtg gcaattgttt ccctgacgat taccgaaaaa ggctattgta ttgacccggc
     2401 caccggtgca ctcgacacca gtaatccgcg gattattcac gatctacaaa cccctgaaga
     2461 acctcactcc gcaccgggta ttctcgtcga agcactgaaa cgccgccgtg agcgcggcct
     2521 tacaccgttt accgtgctct cctgcgacaa tattcccgac aatggccatg tggtgaaaaa
     2581 cgcggtgctg agaatggcag aaaaacgttc gccagaactc gccgggtgga taaaagaaca
     2641 cgtcagtttt ccgggaacca tggtcgaccg cattgttccg gctgcaaccg acgaatcact
     2701 ggcagaaatc agccagcatc tgggggtaaa tgatccctgc gcgattagct gcgaaccgtt
     2761 tatccagtgg gtggttgaag ataacttcat cgctgggcgt cctgcctagg aagtcgcagg
     2821 tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg aaactgcgta tgcttaatgg
     2881 cagccactct tttctcgctt atctgggtta cctctcagga ttcgcccata tcagtgattg
     2941 catgcaggat cgcgtatttc gctatgctgc cagaacatta atgctggatg agcaagcgcc
     3001 gacactgcaa attaaagatg tcgatttaac acaatatgcg gataagttaa ttgcacgttt
     3061 tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg atggatggca gccagaaatt
     3121 accgcaacgt atgctggcag gtattcgcat acatctgggg cgtgaaacgg actggtcgtt
     3181 gctggcatta ggcgttgctg gctggatgcg ttacatcagc ggcgttgatg atgccggaaa
     3241 tgccattgat gttcgcgatc cgcttagcga taaaattcgc gaacttgttg cgggcagcag
     3301 cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa gttttcggtg atgatctgcc
     3361 agataacccg cattttgtgc aggccatcga acaagcctgg caacaaatcg tacaattcgg
     3421 cgcacatcag gcgctattaa acaccctcaa aatttaacga tttctgcggt taaagcggat
     3481 gaagctcacc ttcgtccgct ctccccttct cttttctgcc ttttttagcc aagattaacg
     3541 ctcagttaac ttaccagagt attgcggagc cgttgtgacc aggaccaacc tcatcaccgg
     3601 ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg ttagcccata aagatcccaa
     3661 cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc ggaattgatg gtgctttgct
     3721 cgccgatagc ggcgcattgc tgaaagagat ccccggcggc tgcatgtgct gcgttaatgg
     3781 tttacccatg caggtagggt tgaatacctt actgcgtcag ggaaaaccag accgcttgtt
     3841 gatagagccg accgggctgg gccatccgaa acagatcctc gatctgttaa ccgcaccagt
     3901 ctatgaaccg tggatagatc tgcgcgccac cttgtgcatt ctcgatccac gcctgctgct
     3961 ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag ctggctgccg cagacatcat
     4021 tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag caagcgctac agcgttggtg
     4081 gcagcaaaat ggtggcgatc gacaattaat tcacagtgag catgggaaag ttgacggtca
     4141 tcttctggat ttgccgcgtc gcaatttagc cgagttgccc gccagcgccg cgcattctca
     4201 tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg ccagagcatc aacgctggcg
     4261 tcgcagtctg aacagcgggc aaggatatca ggcctgcggc tggatattcg acgctgatac
     4321 agtattcgac accattggca ttctggaatg ggcgcgactt gcaccggtgg aacgcgtcaa
     4381 aggcgtgctg cgtattcccg aagggctggt acgaatcaac cgtcagggcg atgacctgca
     4441 cattgaaacg caaaacgttg cgccaccgga cagccgtatt gagctgattt ccagcagcga
     4501 agctgactgg aatgccttgc agagcgcgct gttgaagctt cgtttagcga ctaccgcgta
     4561 aggttgcctg cgtttttcag taagataatt agagaaaata tgattaaaaa tttgccgcaa
     4621 atagtgttgt tgaatattgt cggccccgcg ctgtttcttt cctggtatat tcccgttaat
     4681 catggattct ggttgccgat tgatgcggat attttttatt tctttaatca gaaactggtc
     4741 gaaagtaagg cctttttatg gctggttgca ttgaccaaca atcgcgcctt cgacggttgt
     4801 tcactgctgg cgatgggtat gttgatgctg agtttctggc tgaaagaaaa cgcccctggc
     4861 agacgacgta tcgtgattat gggtctggtc atgctattaa ctgcagtggt attaaaccag
     4921 ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa cattgacttt taccgatatt
     4981 aaccgcgtca gcgaactgct ctctgtcccc acgaaagatg cctcacgaga tagctttccc
     5041 ggcgatcacg gcatgatgct gcttattttt tcggcattca tgtggcgtta tttcggcaaa
     5101 gttgcaggcc ttatcgccct tactattttt gtggtttttg catttcccag agtaatgatt
     5161 ggcgcacact ggtttactga catcattgtc ggttcgatga ccgtgatatt gatcggtttg
     5221 ccctgggtgt tgctgacgcc attaagtgat cgattaatca ccttttttga caaatcacta
     5281 ccaggaaaaa acaaacattt ccaaaacaaa taactcacag taattaacat catcagggtt
     5341 atttttatag tgaggataat cctgatgatg cgcaccgtgc tttcatctat cgaacgcaaa
     5401 aatcattctc taagtaaatg aatggattgc atgcgtttca ctcaattgta ctttaattga
     5461 ccaaccccgc ttattaactt tctgtatcac tttttcttat aaaaaatcat gtaaaaccgc
     5521 tcgccaagac cgcaccaatc gggtaatctc gaactcgttt tgcctcggcg gtagattatc
     5581 ctcacagcat ataattttgt gcgttagtcc acaaatttgg ccttaaggaa ttgtttcaac
     5641 atgcccaggt aattagcctc gtgtcgcttg gcattttttt ataacgatat ttgtcgttaa
     5701 ggacttcaag ggaaaacaaa caacatggtc aaatctcaac cgattttgag atatatcttg
     5761 cgcgggattc ccgcgattgc agtagcggtt ctgctttctg catgtagtgc aaataacacc
     5821 gcaaagaata tgcatcctga gacacgtgca gtgggtagtg aaacatcatc actgcaagct
     5881 tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg taaaatcgcg aattatggat
     5941 cagtatgctg actggaaagg cgtacgttat cgtctgggcg gcagcactaa aaaaggtatc
     6001 gattgttctg gtttcgtaca gcgtacattc cgtgagcaat ttggcttaga acttccgcgt
     6061 tcgacttacg aacagcagga aatgggtaaa tctgtctccc gcagtaattt gcgtacgggt
     6121 gatttagttc tgttccgtgc cggttcaacg ggacgccatg tcggtattta tatcggcaac
     6181 aatcagtttg tccatgcttc caccagcagt ggtgttatta tttccagcat gaatgaaccg
     6241 tactggaaga agcgttacaa cgaagcacgc cgggttctca gccgcagcta ataaaccgtt
     6301 tggatgcaat cccttggcta tcctgacgag ttaactgaaa gcactgctta ggcagtgctt
     6361 ttttgttttc attcatcaga gaaaatgatg tttccgcgtc ttgatccagg ctatagtccg
     6421 gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa ttaacggaat catgttcata
     6481 cgcgctccca attctggacg taagctcctg cttacctgca ttgtagcagg cgtgatgatt
     6541 gcgatactgg ttagctgcct tcagttttta gtggcctggc ataagcacga agtcaaatac
     6601 gacacactga ttatcgacgt acaaaagtat ctcgatacct attttgccga cctgaaatcc
     6661 actactgacc ggcttcagcc gctgacctta gatacctgcc agcaggctaa ccccgaactg
     6721 accgctcgcg cggcgtttag catgaatgtc cgtacgtttg tgctggtgaa agataaaaaa
     6781 acattctgtt catctgcgac tggtgagatg gacattccac tcaatgaatt gattccggcg
     6841 ctcgacatta ataaaaacgt cgatatggcg atcttacccg gcacgccgat ggtgccgaac
     6901 aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga aaaatagcgg tgtgtttgcc
     6961 gctctgaatc tcaacctgac gccttctctc ttttatagtt cacggcagga agattacgat
     7021 ggcgtcgccc tcattattgg caatactgcg ctatctacct tttcttcacg tttgatgaac
     7081 gttaatgaat taaccgacat gccggtccgt gaaactaaaa ttgcgggcat tcctctgacc
     7141 gttcggcttt atgcggatga ctggacatgg aacgatgtgt ggtacgcatt tttactgggc
     7201 ggcatgagtg gaactgtcgt tggcctgctt tgctattacc tgatgagcgt acgtatgcgc
     7261 cccggcagag aaatcatgac cgccatcaag cgcgaacaat tttacgtggc gtatcaaccg
     7321 gtggtggata cacaagcttt gcgggtaacg ggcctggaag tactgctacg ctggcggcat
     7381 cctgtagcgg gagaaattcc cccggatgcc ttcattaact ttgccgaatc gcaaaagatg
     7441 attgtgccgc tgactcagca cctgtttgag ttaattgccc gcgatgccgc agaattagaa
     7501 aaagtactgc cggtaggcgt caaatttggc attaacattg cgccggacca tctgcacagc
     7561 gaaagcttta aagcagatat ccagaaactg ctcacttccc tgcccgcaca ccatttccag
     7621 attgtgctgg aaattaccga gcgcgatatg ctgaaagagc aagaagccac acaactcttc
     7681 gcctggctgc actcggtcgg cgtagaaatt gctattgatg acttcggcac cgggcacagc
     7741 gcgcttatct atcttgagcg ttttacgctc gattatctga aaattgaccg tggatttatc
     7801 aacgccatcg gtacggaaac gatcacttca cccgtacttg acgcggtgct gacgctggcg
     7861 aaacgtctca atatgctgac agttactgaa ggggtcgaaa cgccggaaca agcgcgatgg
     7921 ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga ttagtcgccc gttaccgctg
     7981 gacgattttg ttcgctggct gaagaaaccg tatacgccgc agtggtaagg tgtgcttacg
     8041 tcccttatta ttcatagtga aagcatgccg gattgcggct aatgatgagt aaaaggaaat
     8101 ccgttgcaga tgattgtgcg catactgctg ctgtttatcg ctctgttcac cttcggtgcg
     8161 caggcgcagg ctatcaagga aagctatgcc tttgccgtgc tgggcgaacc ccggtacgcg
     8221 tttaatttca accattttga ttatgtgaac cccgccgcgc caaaaggtgg gcagataacg
     8281 ttgtcagccc tcggtacctt cgataatttc aaccgctatg cactgcgcgg caacccgggc
     8341 gcacgcaccg agcagctgta cgacacgtta tttacgactt ccgatgacga accaggcagt
     8401 tattacccgc tgattgctga aagtgcacgc tatgctgacg attattcctg ggtggaggtc
     8461 gctattaatc cgcgcgcccg ttttcatgat ggttctccca ttactgcccg cgatgtagag
     8521 tttacttttc aaaaatttct gaccgaaggc gtgccgcaat ttcgtctggt ctacaaaggc
     8581 accaccgtca aagccattgc gccgttaacc gtgcgcattg agttagctaa acccggcaaa
     8641 gaagatatgt tgagtctgtt ttcgctgccg gtatttccag aaaagtactg gaaggatcac
     8701 aaacttagcg acccgctcgc cacgcctccg cttgccagtg gtccgtacca cgttacgtcc
     8761 tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag attactgggc agcaaactta
     8821 ccggtaaacc gtggacgctg gaatttcgac accattcgct acgattatta cctcgatgat
     8881 aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt tgcgtatgga aaacgacgct
     8941 aaaaactggg ccacgcgtta taccggtaaa aatttcgata aaaaatacat catcaaagat
     9001 gagcaaaaga acgaatcagc ccaggatacg cgctggctgg cgtttaatat ccaacgtgcg
     9061 gtattcagcg atcgccgggt gcgggaagca atcactctcg cctttgactt tgaatggatg
     9121 aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca gttattttca aaataccgaa
     9181 tacgcggcca gaaattaccc cgacgccgcg gagctggtgc ttctggcacc aatgaaaaaa
     9241 gatctaccgc cagaagtctt cacacaaatc taccagccgc cggtatctaa aggcgatggc
     9301 tacgatcgtg acaacctgtt aaaagccgac aaacttctca acgaagcggg ctgggtgctg
     9361 aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc tcagctttga attattgctt
     9421 cccgcaagca gcaatagtca gtgggtattg ccgttccagc acagcctgca acggctgggt
     9481 atcaatatgg acattcgcaa ggtggataac tcgcaaatca ctaaccgcat gcgcagtcgc
     9541 gactatgaca tgatgccgcg cctatggcgg gcgatgccgt ggcccagttc cgatttacag
     9601 atttcctggt catcggaata tatcaattcc acttataatg cccccggcgt gcaaagcccg
     9661 gttatcgact cgctgatcaa ccaaattatt gccgcgcagg gaaataaaga aaaattactg
     9721 ccgttggggc gagcactgga tcgcgtatta acgtggaatt attacatgct gccaatgtgg
     9781 tacatggcgg aagactgtct cgcctggtgg gataaattct cccaacccgc tgtacgccct
     9841 gtttacagcc tgggtatcga tacctggtgg tatgacgtta ataaagcggc caaactgccg
     9901 tcagccagac aacagggaga gtagatgggc gcttacctga ttcgccgtct gttgctggtg
     9961 atcccaacgt tatgggctat tatcactatc aactttttca ttgtgcaaat tgcgcctggc
    10021 ggtccggtcg atcaggccat cgccgccatt gagtttgata atgccggagt attacccggc
    10081 gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg gtgtcggcaa tatcagcgac
    10141 agtaattacc gtggcggacg cggattagat ccagaagtga tcgctgagat cactcatcgc
    10201 tacggtttcg ataagccgat ccacgaacgt tacttcaaaa tgctctggga ctacatccgc
    10261 tttgattttg gtgatagcct gtttcgcagc gcctcggtgc tgacgctgat taaagacagt
    10321 ctgccggttt ccatcaccct cggattgtgg agcacgctga ttatctatct ggtgtcgatt
    10381 ccgttaggca ttcgcaaagc tgtttataat gggagtcgct ttgacgtctg gagtagcgca
    10441 tttatcatca tcggctacgc cattccggcc tttttgtttg ccatcctgct gattgtcttc
    10501 ttcgcgggcg gcagctattt cgacctgttc cctctacgcg gcctggtttc cgctaacttt
    10561 gattcgctgc cgtggtatca gaaaatcacc gattatctgt ggcatatcac gctaccggtg
    10621 ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc tgaccaaaaa ctcattcctt
    10681 gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga aaggggtaag tgagaaaaac
    10741 attctctgga aacatatgtt ccgcaacgcc atgctgctgg tgattgccgg ttttccggcg
    10801 acgtttatca gcatgttttt taccggctcg ctgctgattg aggtgatgtt ttcactcaat
    10861 ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg attatcctgt aatgtttggt
    10921 accttgtata ttttcaccct gattggcctg ctgctgaata ttgtcagtga tatcagctat
    10981 acgctggttg atccgcgtat agattttgag ggacgttaat gtcgcgactc agccccgtca
    11041 atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg ctactggtcg ttatggattt
    11101 tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat cgctaacgat aaaccgttgc
    11161 tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa aaactacagc gaaagcgatt
    11221 ttggcggccc gctggcaagt caggctgatt atcaggaccc gtggctgaaa caacggctgg
    11281 aaaataacgg ctgggtactg tgggcaccga ttcgctttgg tgctaccagt atcaactttg
    11341 ctaccgataa gcccttccct tctccaccct cccggcaaaa ctggctggga acggatgcaa
    11401 acggcggcga tgtgctggca cgcattctct atggcacgcg gatctcggtt ctgtttggcc
    11461 tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc gggggcgcta caaggctatt
    11521 acggcggtaa agtcgatctc tgggggcaac gctttattga agtatggtcg gggatgccga
    11581 cgctgttttt gattatttta ctttccagcg tcgtacagcc taacttctgg tggctgctgg
    11641 caattactgt cttgtttggc tggatgagtc tggtcggcgt ggtgcgggcg gagtttttac
    11701 gtactcgtaa tttcgactac attcgtgcgg cacaggcgct tggcgtcagc gatcgcagta
    11761 tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac cctcaccttt ttaccgttta
    11821 ttttatgtag ttcgataacc accctgacct cgctcgattt cctcggcttc ggtctaccgc
    11881 tcggttcacc gtcactcggt gaactgctgt tacaagggaa aaataacctt caggccccgt
    11941 ggcttgggat caccgccttc ttgtcggtgg cgatattatt gtctttgctg atctttattg
    12001 gtgaagccgt ccgcgacgca tttgatccta ataaggcggt gtagcatgac gcaaactctg
    12061 ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc aaaccgtacg tacagtagtc
    12121 aatgatgttt cactacagat tgaggctggc gaaacgctgg cgctggtggg tgagtcaggt
    12181 tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc tcccttcccc gccggttgaa
    12241 tatctctccg gcgatattcg ttttcatggc gaatcgctgc ttcatgccag cgatcaaacg
    12301 ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc aggaaccgat ggtgtcgtta
    12361 aatccattgc ataccctgga aaaacagctt tatgaagtgc tttcactcca ccgcgggatg
    12421 cgtcgggaag cggctcgtgg cgaaattctt aactgccttg atcgcgtcgg tatccgccag
    12481 gcggcaaaac ggctgacaga ttatccgcat cagctctccg gcggcgaacg ccagcgggtg
    12541 atgattgcga tggcgctgtt aacgcgaccg gaattattaa ttgccgatga accgaccacc
    12601 gcactggacg tctctgtcca agcgcagatt ttacagctgt tgcgcgaact gcaaggcgag
    12661 ctgaatatgg gcatgctgtt tattactcat aacctcagca ttgtcagaaa actggcccac
    12721 cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa ataacgccgc tacgctattt
    12781 gcctcaccca ctcatcctta cacacaaaag ctactcaaca gtgaaccgtc aggcgatccg
    12841 gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg aacagcttca ggttgccttc
    12901 cccattcgca aagggatttt gaagcgcatt gtggatcata atgtggtggt gaaaaacatc
    12961 agttttacgc tacgggcggg tgaaacactg ggtttagtgg gcgagtctgg ttccgggaaa
    13021 agcacgacgg gactggcgct gctgcgactg attacctctc agggcagcat cgtctttgac
    13081 ggtcagccac tgcaagattt aaatcgccgc cagctgttac ctactcgtca tcgtattcag
    13141 gtggtatttc aggatccaaa ctcctcgctc aacccacgac tcaacgtttt gcagattatt
    13201 gaggaaggct tacgggttca ccagccgacg ctttctgccg cacaacgcga acaacaagtg
    13261 atagccgtga tgcatgaagt gggattagat cctgaaacac gccaccgtta tccggcggag
    13321 ttctctggtg gtcagcgaca acgtattgcg attgccaggg cattaattct taagccctcg
    13381 ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa cggtacaggc gcaaatattg
    13441 acgcttttga aatcattgca acaaaagcat caactggcct atttgtttat cagccacgat
    13501 ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac tgcgacaagg ggaagtagtg
    13561 gaacaaggac cgtgcgcgcg cgtgtttgcc acaccgcagc aggagtatac gcgtcagtta
    13621 ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa aagggttcgg caattgccac
    13681 accaaaattt ttcaatcggc atgttgcagc aaactcgtcc tggcgattta caaacaggca
    13741 cggctcacct tcacactcca gcaccgaaca tggaacttcg atgtcgctta acgcctggct
    13801 tagtttatac atcaccgacc atgcattatc accatccggg ctaagcaatt taagcgccac
    13861 caggctgttg tcaccgcacg gtccattttg cggaatcggt tcaaccttcg aacctgcgaa
    13921 accagcagac cagcgatagt tctgcggcag tcgatggacg attgagagtt gtaatgaagt
    13981 cacttatttt ccccggaagt acattcactt cacaatttgt ttacatcaat tttaacacat
    14041 catcaacaat ccgtctttat acagatcgta aagattgatg cctcgcctgc gtggcctcta
    14101 tggctctgat ttaagtaata tctgcgggcg tggtcacgtt tgcggggcta tatgtacccg
    14161 tttctgcgat ctaactcaac ctttttaact acaatgatgt gactttttac ataaattgat
    14221 tttacataaa ataaacatat atcggggaaa tgatagattt gtggttgaca tgcatcaaca
    14281 aaggaagcct tttaccttcc tcgttgtgca atagatcacc gttttttcgg ccgactggcg
    14341 tacagacaga agagaatgga gctggttgcg cagaatgcaa ttgaccaaat catcggccat
    14401 gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa ttgcccctat gccaaaacgg
    14461 aaggttcctg ccagcgaaga tgccgttccc gccatatggg gaaactcatc aagaatgacc
    14521 gccatcgcat tggatgacac catcgacacg cagcccacaa acgccgcaac gccaaccacc
    14581 agcgaccaga atcccagccc cagcagcgca ctgatgacca tccacgctgc cataataaat
    14641 tgtatccaca accccgagcg gaacatattt aacgcgccaa tgcggcggac gaagcggctg
    14701 ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg caaagtaata accaaagttt
    14761 tccggcgcga cgtggttaat ttcaatataa acaaacggtc cggcgcttaa gaatgagaac
    14821 atcccggcaa agctgaaacc actggcaagc atgtagctca ggacacgttt atggcggaac
    14881 agcgccgcaa agttaccaat agtggtacga atgtgaaatg gctgacgacg ctccggtggt
    14941 aaggtttctt taatcaggaa gaaaatcatt gccgaagcca gaatcgccgc taatgccagg
    15001 atccagaaga tgtaatgcca gctcagccac accagcaccc agccgccaac tatcggtgcc
    15061 atcagcagtg caatggtcgt caccagcatg acaaacgaca tcatccgcga gaactcttct
    15121 ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc tggccgcagc cgcagccagc
    15181 ccgtggaaga aacgcatcac aatcagctga tcgatggttt gtgccaacgc acacgccacc
    15241 gcggcggcgg caaacaccag cgtcccgcca agaaccaccg gcttacgccc gaagctgtct
    15301 gccatcggcc cgtagattaa ctgccccaac gcaaagccca gaatataagt actgagggtc
    15361 atctgcgtac tgcccgccga tacgccaaac tgcgctgaaa ttaccggtag cgcgggcaga
    15421 tacatatcaa tcgacagcgg catcaacatg gccagcaggc caaggataaa aacaatagca
    15481 aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa agtcattgaa aagttagacg
    15541 acgctggcaa tttcttcttc agttaacgga cgatattcac cgggggctaa atcagcatcc
    15601 agcgtaatac cgccaatacg ttcacgatgc agctcaacca cgtggttacc cacggcggcg
    15661 aacatgcgtt tcacctgatg ataacgccct tcgctgatgg tcagacgaac ctgcgttggg
    15721 gtaatcactt caagcaccgc cggcttagtg agatcttttt cgttatgcag ctgcacgcct
    15781 ttagcaaatt gctctgccgt atcgtcagct acaggtgatt ccagtgtcac cagataggtc
    15841 ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc actgaccatc atcagtcatc
    15901 agcaccagac cggtggtatc gatatccaga cgtcctgcgg cgtgcagttt ccacgctacc
    15961 ggttcatcca gaaaatagag caccgtcggg tgatccgggt cgtccgtgga gcaaacatag
    16021 ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt gctgcgccag cgggttgcca
    16081 tcgtaagcga catcatgttc agggagcagt ttgaacgctg cattacgaac gatttcgcca
    16141 tcgacggtga cacgattgcc gcggatttca cgcccggcaa tagcacggct aacgccgagt
    16201 tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt tgcctgtaaa aaacggaggt
    16261 cgggtaatga cccgaaaatc tgaactgttg tctgcccagt atagcggtct aataacttcc
    16321 ctcaagggaa aaagaaccgt ggcatactat tagcagaata atctacctac gcgaaaccat
    16381 gatttttaca ctccgcccat atcagcaaga agccgtggat gccacgctca accattttcg
    16441 tcgtcataaa acccctgccg ttatcgtgct gcccaccggc gcaggtaaaa gcctggtgat
    16501 agcggaactg gcacggctgg cgcgtggtcg cgtactggtg ctggcacacg ttaaagaact
    16561 ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg gaagccgata tttttgccgc
    16621 cgggctaaag cgcaaagaga accacggtaa agtggtattt ggcagcgttc agtcggtcgc
    16681 ccgtaatctt gatgcctttc agggtgaatt ttcgctgttg attgtcgatg aatgccaccg
    16741 tattggtgac gatgaagaga gccagtatca gcaaatcctc actcacctga ctaaagtgaa
    16801 tccccactta cgcctgctgg ggctgactgc cacgcctttt cgattgggca aaggctggat
    16861 ctaccagttc cattatcacg gcatggtacg cggcgatgag aaagcccttt tccgtgactg
    16921 catttatgag ctgccgctgc gttatatgat taaacacggc tatctgacgc cgccagaacg
    16981 actggatatg ccagtagtgc aatacgattt cagccgcttg caggcacaga gtaacgggct
    17041 gttcagcgaa gctgatctca accgtgagct gaaaaaacag caacgcatta cgccacatat
    17101 catcagccag atcatggagt tcgccgaaaa gcgcaaaggg gtaatgattt tcgccgccac
    17161 cgttgaacac gcaaaagaga ttgtgggatt actgcctgcc gaagatgctg cactgattac
    17221 tggcgacacc cccggcgctg agcgcgatgt gttaattgaa gattttaaag cccagcgttt
    17281 tcgctatctg gtcaacgtcg cagtactgac caccggattt gacgccccgc acgtcgatct
    17341 tatcgccatt ctgcgcccta ccgaatcagt gagtctttac caacaaattg tcgggcgcgg
    17401 tctgcgtctc gctccgggca agactgattg tttaattctt gattatgcgg gtaatcctca
    17461 cgatctctac gcgccggaag ttggtacacc gaaaggcaaa agtgacaacg ttccggtaca
    17521 ggttttctgc cctgcctgcg gttttgccaa caccttttgg gggaaaacga ccgccgacgg
    17581 gacattgatt gaacactttg gtcgccgctg tcagggatgg tttgaagatg acgacggtca
    17641 tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc ccgcaatgta acgcagaaaa
    17701 tgatattgcc gctcgccgct gccgcgaatg tgacacggta ctggttgatc cggatgatat
    17761 gttaaaagcg gcgctacgac tgaaagatgc gctggtatta cgctgtagcg gcatgtcttt
    17821 gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc acctattacg atgaagacgg
    17881 cgcggatgtg agtgagcgtt tccgtctgca aacgcccgcc cagcgtaccg ccttcgagca
    17941 gctttttatc cgcccgcata cgcgcacacc gggcatccct ctgcgctgga tcaccgccgc
    18001 cgatatcctc gcccagcaag ccttactgcg acatccggat tttgtcgtcg cccgcatgaa
    18061 aggtcagtac tggcaagtgc gtgaaaaagt gttcgattac gaaggtcgtt ttcgtcgggc
    18121 gcacgaatta cgcggttaat ggtagttttg attgatgtat aaggcgattg agtatagaat
    18181 ctcgcccgct tttgcatacg caaagcagat cacttacttg ttgctgggtc gcctgtagca
    18241 ggattaattt aagagagaaa gaaatgttta ctatcaacgc agaagtacgt aaagagcagg
    18301 gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt cccggcaatc atctacggtg
    18361 gcaaagaagc accgctggct atcgagctgg atcacgacaa agtcatgaac atgcaagcta
    18421 aagctgaatt ctacagcgaa gttctgacca tcgttgttga cggtaaagaa atcaaagtta
    18481 aagctcagga cgtacagcgt cacccgtaca aaccgaagct gcagcacatc gacttcgttc
    18541 gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg gcggggtttt ttgtatctgc
    18601 agattatgcc tgattacggt attgctattt tttgcaggcc agataaggcg ttcacgccgc
    18661 atctggcatt cagaacacaa aaccgattaa ttgccgccag acgtccggcg ctgcaattgg
    18721 tcgcgcaaat tcggcggcgt gcctttaatg gtcaaggtat cggttgctgg atcccagaag
    18781 atgcgttcac ctaacagcat cgcatcaaag ttaatagtca agccaccgcc gctaccggca
    18841 aacttcgtca actgacgcag tgtgctacgg tctgccggga agctctcttc cagttcgtag
    18901 cctttttcag ccgcaaactc cgtgaagctc acttcgctta cgcctgccag ctctttcgac
    18961 agcgattcca gttcgatctc ttcgcctgcc tgcagctgct cattacagta gctataaacc
    19021 tgctggcgca cgttctgtcg ctctgcttta tccagttgtg cttcggcagt gaaatcatca
    19081 accgcttgca acagcccacg gttttgcgct ttcgcgttca atccttcgct ggccccgagg
    19141 aaatccataa agaaatccgc cactttgcgc cctactcgcc ctttcaggaa agtgagatag
    19201 cgggtggact ctggattggt ttcccattcg gttaaatcga tacgcgcaac aatatccgca
    19261 tgattgatat caagataatg ggttgggttg atgtcgagat tttcgttaac gcgcatactg
    19321 ctcaggttgc tcagaactgc caccagcaaa tactccaccg ccagataacg gtagtggcaa
    19381 aacagcacga atccaccgtc agcaaaagga tatttcgcta attcgtcacg caggcgaccg
    19441 gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct gacgctgtaa gcgcagcgtc
    19501 tgcgccagtt cactctcttc gctaaacaag ccgtaagctt tatttttggc gctatagacc
    19561 cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg gttccagcaa tgaatcgcgc
    19621 aacaccagct caaggttctg ctcatcacgc ttgataagct ggtgcagagc aatctggttg
    19681 atatccagac tcatgataaa ctctccttta agaccgggcg gtattcaacc accgccggtt
    19741 aagcgacgca ataaaagatg gcggtcattt tacagaaggc agcgtaatga gaatgattta
    19801 acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat atgttgccct ttcatcaact
    19861 aactgatttc gatttatgcc acaaatttcc cgctacagta atgaacaggt tgaacaattg
    19921 ctcgcagagc tgctcaacgt actggaaaaa cataaggctc cgaccgacct ttccctgatg
    19981 gtgttaggca acatggtcac taaccttatc aacaccagca ttgccccggc ccaacgccag
    20041 gcaattgcca actcttttgc ccgcgcctta cagtcctcta tcaacgaaga caaagcgcac
    20101 taagggaaac agataacagg ttatggtaac tcatcgtcag cgctaccgtg aaaaagtctc
    20161 ccagatggtc agttgggggc actggtttgc actgttcaat attctgcttt cgctcgtcat
    20221 tggcagccgt tacctgttta tcgccgactg gccgacaacg cttgctggtc gcatttattc
    20281 ctacgtaagc attatcggcc atttcagctt cctggtgttc gccacctact tgctgatcct
    20341 cttcccgctg acctttatcg tcggctccca gaggctgatg aggtttttgt ccgtcattct
    20401 ggcgacggcg ggaatgacgc tattactgat cgatagcgaa gtctttactc gtttccatct
    20461 ccatcttaat cccatcgtct ggcagttggt tatcaaccca gacgaaaatg agatggcgcg
    20521 cgactggcag ctgatgttca tcagcgtgcc ggttatttta ttgcttgaac tggtgtttgc
    20581 gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt cgacgcttcg cgcgcccgct
    20641 ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg gtgtatatct gggccgatgc
    20701 caacttctat cgcccgatca ccatgcagcg cgctaacctg ccgctttcgt acccgatgac
    20761 ggcgcgacgt ttccttgaga agcatggcct gcttgatgcg caggagtatc aacgccgtct
    20821 tattgagcaa ggtaatccag acgccgtttc cgtacagtat ccgttaagcg aactgcgcta
    20881 tcgcgatatg ggcaccgggc agaatgtgct gttgattact gtcgatggcc tgaactactc
    20941 acgcttcgag aagcagatgc ctgcgctggc aggttttgct gagcaaaata tttcgttcac
    21001 gcgccatatg agctccggca acactacaga caacggcatc tttggcctgt tctatggcat
    21061 ctcgccgagc tatatggacg gcattctgtc gacccgtacg cctgcggcat taattactgc
    21121 gcttaatcag caaggctatc agctggggtt attctcatca gatggcttta ccagcccgct
    21181 gtatcgccag gcattgttgt cagatttctc gatgccgagc gtgcgcaccc aatctgacga
    21241 tcagaccgcc acgcagtgga tcaactggct gggccgctac gcacaagaag ataaccgctg
    21301 gttctcgtgg gtttctttca atggcactaa cattgacgac agcaatcagc aggcatttgc
    21361 acggaaatat agccgggcgg caggcaatgt cgatgaccag atcaaccgcg tgctcaatgc
    21421 actgcgtgat tctggcaaac tggacaatac ggtggtgatt atcactgccg gtcggggtat
    21481 tccactgagc gaagaggaag aaacctttga ctggtcccac ggtcatctgc aggtgccatt
    21541 agtgattcac tggccaggca cgccggcgca gcgtattaat gctctgactg atcataccga
    21601 tctgatgacg acgctgatgc aacgcctgct gcatgtcagc acacctgcca gcgaatattc
    21661 acaaggtcag gatttgttca accctcaacg ccgtcattac tgggttactg ctgcggataa
    21721 cgatacgctg gcaattacca ccccgaaaaa gacgctggtg ctgaacaata acggtaaata
    21781 ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa aaaccacagt taagtttgtt
    21841 attgcaagtg ctgacagacg agaagcgttt tatcgctaac tgattaatta taaatcagtt
    21901 agcgaaatat cttacttgca atcggtgtgg aaaacggtag tattagcagc cacgagtcgg
    21961 cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg ggggtcggag gttcaaatcc
    22021 tctcgtgccg accaaaaatc ccaagaaaaa accaaccctt acggttggtt tttttatatc
    22081 tgcaattaat tcgataaaca gaccgtgaca catcacagcc tgtttatttt ctgttatcag
    22141 aacgtccaga ccacacccgc ctgagtattc cacggcgatt ctacccctgc gccattgcca
    22201 tagctgactg acaaatgacc gcttaacgtc ggggtaaacg atgaccttat cccagcctga
    22261 taaacgccac tggttccagc cacatcatta ttaaatttgc catcgtcatt aactttcacc
    22321 aggttagaat cggcgtattc ctgacgcacg gcggctttca gccagggttc cagcgtcgta
    22381 ccgttttgca ggtccatgtg atagcttacc gccgttcccg cttcagcgcg taatatccgg
    22441 gtatttccca catccgcgcg catgccgttt gataacgtgt agtcctgacc atctgtggta
    22501 aagccggtaa aggccagata gggtctaaca ctccacaatc cgtcaaccca acggaacccg
    22561 ctctcaacat gagcacccgc gccgttacta ttgtaatcgc caaacgctgt tgccccatta
    22621 ctcatattgc catggatggt gttggcaaaa cggtcaactt tcaccacccc atcaacatag
    22681 gcaccgttct gatgctccca accggcataa gcccccaggg tatagctatc gatattacct
    22741 tttccgccgc gatcaaaacc aatatcagaa tgagagtaac caaagaacaa gccgcgaatt
    22801 gtactgcttt cttcacggga gaaacggcta tcgataccga gcgtcaggcc cgtcaatgtt
    22861 tgctcaaaac cagctcccgc atcagtggtc acgttgttgc gggtgttaat tgccgaactc
    22921 cacatcaccg tatcgtaact aacgcctttt acgctaccaa gacgctcacg cacggtgtcc
    22981 agttctgcat caaataccag cggttgtgcg gctgccatgt tcagcacatc agtggttgaa
    23041 ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat ggttgccatt atccagcaag
    23101 gtatattcat acgtaccgat atcaacaacg cctccggcat tgcccaacgt aaatgcagca
    23161 tcaccgccgc ccgttgttac cagtgtaagg ctatctcctg ctgccgggct ggcaccggtg
    23221 tccgtcacga atattttgaa atcacctgtt gcctgaccgg tgacgttgag ctgatcgctc
    23281 tgatgattgg ccatatcggt acgcatataa aaattgccgt ttcccgacag ggtattggtg
    23341 gtcaacgtaa tgaaattttc cgcacttgcc gtcacagatg aacgagtcac tggctcagcc
    23401 atcataatca catttgcgcc ggacatcgca aggttggcta agttgaggcg agtgttttgg
    23461 ttcgtcgtag tgatatcggc aatgatggtc catgcgctat tttcgagcga aacgtccgct
    23521 ttgctggtat ccacggcacc atgcgttcta aaagaagcgc cctcagaaac cactacctta
    23581 cccaccaaca tagcgggtgc ataatttatt tgcggcttca tgacctcaag aatgccatca
    23641 ttccctctga ctgacacgtt aaccattgtg ccagcccaga cattagcgcg gccattgttg
    23701 attaccatat tttcagagat ggcttttgag gaatacgtga tatttccatt ctggtctgat
    23761 cgaccgagat catataccgc atttgcctgc actttcgtac cagtatcctt tcccagcgat
    23821 tgcatagtgg catgctcatc aaggatagtg tcaatggcct gcgtgtcctc cataacaatg
    23881 agcccggaac catcatccag ttcataattt tttgacacac catcctttat actaaattgg
    23941 cctttactgt ttgtaccact cacttccagc gcattcgttg agacaattaa tttcccgcct
    24001 gcttttttat caacgccagt tgcagtcccg ccatccataa cctccagtaa gccgccacta
    24061 tccacagtag tattataagc gaagtcattt tcttcaactc gcaaacttcc gccgttttcc
    24121 aacaacatat tattagcaat accattcttg atatcgaact gaccgagacg gtttgttccg
    24181 aatacctcca tggcccgcgt ggttgtttta atagcaccgc ctgctttctg atctaccgca
    24241 agcgcaaatc ccccctcttt aacctccagt agtcctccct tttcgatagc ggcaaaaacg
    24301 gtccccccat ctttttcaac gattaagcgc ccgcctttaa tcgtggtatc attcgctggg
    24361 ccataaacct gtagcacacc attattgagg gtagtctggt ttgccgcgcc acccatttga
    24421 acaatttgat aaccatcatt taatactgag tattctgcgg taccattcat ttcaatgtat
    24481 tgataccctc cgttaatttc agtattacta cttacaccaa attcttttat atgctgttct
    24541 ccgccaggat tgattttcgt attattggcg gtcccataaa ccagctggat atctttttcc
    24601 aggataacac catcaactgt ctcatcatac tcaactgttg atgccaatgc tatgggtgaa
    24661 tataacgctg tagccaccag aacagatatt gcggaacgac aaagagaaac agaaccagat
    24721 tgatgcattg agctttcatc ctatgaaatt aattgcagtt aaaagcattg ggtacagaaa
    24781 atacccatag ctccataccc ggagtcagtt tttaaaaact gtttaaagaa atgcacaagt
    24841 attgtgattg attttttagt tgtttttctt gatgagaagc tgatgcaaaa ttccgtcttt
    24901 ataatgaaaa tgatgccaaa gcgaacgaca aggttgtagt tttcactaca tgtccataca
    24961 taaaatgggg taacattcac gcgcctggta gcgttaccaa cgctacgctc aaacataatg
    25021 attctaataa aacctcagga gactactatg cctgaagcaa cgccttttca ggtgatgatt
    25081 gtggatgatc atccacttat gcgacgcggt gttcgtcagt tactggagct tgatcctggc
    25141 tttgaagtgg tcgccgaagc gggcgacggc gcgagcgcga tcgatctggc gaatagactg
    25201 gatatcgacg tgatcttgct ggatctcaat atgaaaggta tgagtggcct ggataccctc
    25261 aatgccttgc gcagggatgg cgttaccgcg caaattatta tcctgaccgt atccgatgcc
    25321 tccagcgatg tctttgcgct gatagacgca ggcgcagacg gttatctgtt gaaagacagc
    25381 gacccggaag tattgctgga agcgattcgt accggagcga aaggcagcaa agtctttagc
    25441 gaacgcgtca atcagtactt acgtgaacgt gaaatgtttg gcgcggaaga agatcccttc
    25501 agcgtgctga cggagcgcga gctggatatt ctgcacgagc tggcacaggg gctgtcaaat
    25561 aaacagattg catcggtgtt gaatatttcc gagcagacag taaaagtaca tattcgcaat
    25621 ctgctgcgta aactcaatgt ccgctcacgc gtggcggcca ccattctgtt cctgcaacaa
    25681 cgcggggcac aataaaaata gcccgatgga tttatcatcg ggctgagatt tatgacaaac
    25741 gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg gatcgatcaa tttattgaat
    25801 ttacacaatt ttgtaggccg gataaggcgt tcacgccgca tccggcataa actaagcgca
    25861 ctttgtcatc agtctagccc gatggcatca ccatcgggcc tctttttatt tactctcctg
    25921 cggcgacaaa tgttgcatcg cctgcgcgat actacgttca atcaccgcac ggcgagtatc
    25981 gttggcaggt aagagtttca acatcatctc ccacgcggca acggcttcgc caaatcgctg
    26041 ctgctcaaag gcattaaacg catacatgct tagcacacgg atattgctat ggtccgttct
    26101 caccagctga cgtagcagtt caccgccgag gcggttgtcg ttgggatcag atgaacgagt
    26161 caacgcttca gcgtatccca gtgcggcatc gctgttcttc ggatcgaggc gatacgcagt
    26221 agcgtatgca tcggtggcga tactggcgtt acccagcgcc atgccaacgc ggcccaacat
    26281 aatccagcct tctatatctc ccggattttt ttgcagttga gtacgcatcc ccagcgcaag
    26341 gcgcgacatc tcttcttcgt tgagcggatc ggctttcggg tccagcgccc tgtccagcaa
    26401 cgccggagcc tgtgccgtgg cctgctgcca gattttcacc tgctgataat tgccagtctg
    26461 gtagtagctg acgccagcca caattaacgc caccacaata cccggcagat aaacaacata
    26521 tccggcacgc ttaccttccg gcacgctttg ttcaggaaac gcttccggca ccacgcgtac
    26581 ccgacgccgc gagcgggcgt atatgaccca accgccaatg ccaatagcca ctactggcag
    26641 cacccacagc agcacggtca gcggcgttaa cggcggatcg taagtgacga agttgccgta
    26701 acgcgccacc atataatcga caatctcttt tttactttta ccttcctgca tcagttcata
    26761 cactttctga cgcaggtcgg tggcaatcat cgagttggaa tcggcaatgc tgttgttctg
    26821 gcatttcggg cagcgcagtt cttcagtgag ctgacggaac tgttgttcct gtgcttcatc
    26881 tttaaactgc aacacgtcga tggtcgccag cgctgagccg aagatcatca gcatcagcac
    26941 gcccaataaa aacctcattg tgcggcctcc ttactgtatt tctcccacag cggcttgatc
    27001 tcttcttccc agacgcgagg attgagatcg cccgcatggc gatagcgaat gataccgttg
    27061 ccgtcaataa ggaaggtttc tggcgcgcca tagacaccga gatccagccc taacatgccg
    27121 tcgccatcaa acaggcttag cgcgtaagga ttgcccagct ctttcagcca gctgattgcc
    27181 ttctggcgat cgtctttata gttcatgccg accacgcgaa tgccctgcgc tgaaagctga
    27241 ttcagatatt gatgttccgc acggcaggtc ggacaccaag tcgcccagac gttaagcagt
    27301 actggtttgc cctgagtcag cacatccgcc tgataaaact gccccggatt gtccagtgat
    27361 tcgagacgaa acttcggcac aggcttgcca atgagcgccg attccagatt ggtcggatca
    27421 tccccttcgg cattacgcgc cagctgccac agcagcgccg cggcaatcgc caggaagata
    27481 atcaacggaa ttaacaatac tttgcgcttc atacggcctc cggcgcagtt ttttgcggac
    27541 tcacgcgctt acgatagcga ggatcaaaca gacacagcag tccgcccaac gccatcatca
    27601 gcccgcccgc ccaaatccag cgaacaaatg gtttgtagta aagacgcacg gcccacgcgc
    27661 cgttttccag ctcttcaccg agcgcggcgt acaggtcacg cgtgatgccg ccgtcaattg
    27721 ccgcttcggt catcatcgac ccggcagtgt tgtaataacg tttttccgca tacagcaccg
    27781 tttccggttt accctctcgc gttacgccaa tagtcgccac accgccgcgc cagttcgggc
    27841 cagtcacctc tttgacatca cggaaggtga agcgatattc atgaatatcg acgctatcgc
    27901 cggacttcat gcgcacatca cgctcaacgc tataattctg gctaaaggca atgccaacaa
    27961 ttgtcactgc cagccccagg tgagccgcca ccatccccca ataactgaag gtggttttcg
    28021 tgccgcgtga aatacgcagc gcagcttccg cagttgccag caccgcaatc cagcaggcca
    28081 ttgccaggcc gagcaccgtc atcgccacaa ctttgctttc gaacagccac ggcaacagca
    28141 gagacagcac cagcgtagag atgaaggcga taatcaataa attgcggatt ttacgtgggc
    28201 gatcgcgccc ccagcgcacc agaggaccga caccaagcag tagcgcaaac ggcaccatca
    28261 gccaggtaaa catggtgttg aagaacggtt cgccaatcga aatactgccc agtccaagtt
    28321 gcttgtgcac cagcggcagc agcgtcccca gcaacaccac cagcatcgcg gcgaccagca
    28381 aaacgttgtt cgctaacagc aaagattccc gcgaccacag cgcattgttt acgcgtgagc
    28441 gaactttgtg tccacgcgcg gcaaacagca gcagcgaacc gccaatcacc agcaccataa
    28501 aggcgaggat aaacataccg cgcgccggat cagacgcaaa cgcgtgtacc gataccagca
    28561 cgccggaacg cacgaggaaa gtccccaaca gacacaacga gaaggcactg attgccagca
    28621 gtaatgtcca cgctttgaag ctggcgcgtt gttcagtgac cgccagtgag tgcatcagtg
    28681 cagtccccac cagccacggc ataaacgagg cgttttctac cggatcccag aaccaccagc
    28741 caccccagcc gagttcgtaa taggcccatg cggaaccgag cacaatgccg agcgtcagga
    28801 aaatccacgc cgccagcgtc cacgggcgag taaaacgcgc ataagtgctg tccagacgcc
    28861 cgctcagcaa agaagcaatg gcaaaagcaa acgccaccga gaaacccacg taccccatat
    28921 aaagcagagg cggatggaag atcagccccg gatcctgtaa cagcgggtta agatcgcgcc
    28981 cttcaatcgt gaagttcggc agcgtgcgtg agaacgggtt agaggtaaag agaatgaaca
    29041 gcaaaaagcc gacgctgacc atccccatta tcgccagtac acgggccaca atatccagcg
    29101 gaatacgctg actaaaaatc gccaccgcaa aggtccagcc gctcatcagc agcacccaca
    29161 gcagaagcga accttcatgc gcgccccagg tagccgccac gcgataccac accggaagct
    29221 gggtattgga gttgctggca acataggtga cggtaaagtc gttgaccacg aaagcattga
    29281 ccagcaccag aaatgcgcca gccacagaca taaacagcag ccaggcaaac aagcgggaag
    29341 acgccatcat gcgcgcatct ccgcgcgcca cgccccatag cggatacacg gacagcagca
    29401 gcgcaatccc taacgccaag cacagcagcc cgtttccaat ttccggcatc atgatgctgg
    29461 gtccttataa acactcgccg ggcgacggtg gttagcttcc atcgctttct caacttctgg
    29521 cggcgtgtag ttttcgtcgt gtttcgccag cacttctttc gcgaggatat gattgccttt
    29581 ttccagctcg ccctgcacca caacgccctg cccttcacgg aacagatccg gcaaaatgcc
    29641 ttcgtaagag acatccactg agccttcagc atcgtaaatg gtgaaggtca ctttcagcga
    29701 attgggatcg cgctgcacgc tacccggcat caccatcccg ccaacgcgca gacgctgacc
    29761 gacttccggc atctgctgag tttcacgctt gccgtagaga atttcccccg gcgtataaaa
    29821 gagatcgata ttcgagcgca gcgcatatag caccagaccg atagtcagcg ccagccctgc
    29881 caacacggca caggcaatcc acaagcggtt tttacggcga atattcatgc agcctcctgc
    29941 tgttgcgcag cacgtaaacg cgcctcacgc gcccgctgtt gcgccacacc acgcagaatt
    30001 gcgcgatgtt gcatcaccga gtgcacgacc aaaaccacca gcggaataac ggtcatcacc
    30061 accgccagcc agacaaaaaa ggcgtaaccg cccattgcga aaaattcatt ccaggaagca
    30121 aatgcagggg tcatttacgg cctcttttca gtatcagttc actcacccac ggacggcgtt
    30181 tttccatcag caaaatcaaa ttacgcatcc gcatcagcgt cagcgtggca gacaggagca
    30241 ggaagccaaa aatcgaccag cgcagcggcg aacgcatcgc cggatcgata ctttgctgca
    30301 tccgcgttga tccctgatgc agggtgttcc accactccac ggagtaatga ataatcggca
    30361 gattcaccac gccaatcagc accaggatac ctgccgcacg gcccgccaga cggcggtcgt
    30421 cgaaggcgtg ccacagggca atcacaccca catacaaaaa cagcagcacc agttcagaag
    30481 tcagacgtgc atcccatacc caccaggtgc cccacatcgg ttttccccat gcagagccgg
    30541 taaccagggc aataaaggta aacacggcac caatgggggc catcgccgcc accgccaggt
    30601 tggccatttt catctgccag acaaggccaa taaacgccgc caccgccatt gatgcataaa
    30661 tgcccatcga ccagatcgcc gcaggcacat gcaggtagat aatgcggtag ctatttccct
    30721 gctgataatc agccggagcc aagccgaatc cccagatcca gccgacggta agcacgacca
    30781 cactggcaat tgccagccac ggtataaacc agccacagat ttgatacagc cgtggtggga
    30841 tcgccagttg atgcagtgtt ttccacatag tttcgatacc agactcgaac aaaaatcagt
    30901 aatccagcgt tattgaatgc tgattcgtaa cgctgccgcc gtcgcaaaag gacttaatgt
    30961 cgcggtgcct gccagcaacg cgcctaaaat tgccagatac ccatcaacgg gcaaatgcat
    31021 agaagccgcg tccatcgcgg cggtggcaaa gatgagtaat gggatagtca gcggtaacac
    31081 cagtatgctg agcagcacac caccgcgctt aagtcccact gtcagcgcca cgcccggtgc
    31141 accgagaaag ccaagcgtag gcgttcccag cagcagcgtc agcgccatca cttgccagcc
    31201 ataaacatcc attcccagta gcattgctac cagtggcgaa aggatgagta acggcagacc
    31261 ggttaccatc cagtgcgcca tcaccttcgc cagcacaacg gcgggcaagg gtaacggcaa
    31321 caacatcaat tgttcaagac tgccgtcctg caaatcgtca cggaacagtc gttccagcgc
    31381 cagcaaggat gaaagcagcg cagcaaccca gataatgccc ggtgcaatac gcgccagcag
    31441 ttgcggctcc ggaccgatac tgagcggaaa aagggtaatt acaatcagga agaaccacag
    31501 cgggttggcg atttcggcgc tatggcgaaa cgctacgcgc agttcaagac ggaaaatacg
    31561 ccagaacatc atgcggccct cgtttgcgtc agtgaaatgc ggcgaatttt actttcagca
    31621 acgttgagcg gctggtgggt agtcagaatc acaatccccc cctgctccgt atgctgcgcc
    31681 atacgctggg tcagacgatc gacgccgtta acgtcaatcg cggtaaaagg ctcgtcgagg
    31741 atccataatg tggcacgggt caaccacaga cgcgctaaag cgacgcggcg ttgttgcccg
    31801 gcagaaagtt gatttaccgg aatatcttcg aatccggcaa gcccggcctg cgccagggct
    31861 tccagacatt gtgcggcatc gccatcgcga tgataaaagt gcagattttc taacgccgtc
    31921 agccgggttt tgatccccgg ctgatggcct atccataaca ggttttgatg gtagctgtcg
    31981 cgtacctgat gcaagggctg cccttgccag agaacctcgc cagcgtcagg gcgagacaac
    32041 cccgtcagca aacggagaag cgttgtcttc cccgcgccgt tgctaccggt gatttgtacc
    32101 cactctcctg cgttcagcgt aaatgacaag ccactaaata aggttcgttc atcccgctca
    32161 caaagtaact ctctggcttc aagcataccc acgcaataac cctgttaaaa acctggctcg
    32221 acttcacgca tatcgggcag cttgtgcgcg atccctttat ggcaatcaat acaggtttgc
    32281 ccatctttca ccgcctggtc atgcatcttc gcggcaaccg atttctgggc ggttgtatcc
    32341 atatactcga agttgtgaca gttacggcac tcctgcgagt tattgtcctt catgcgccgc
    32401 cactcattct gtgccatcgt cagacgatga gcttcaaatt tctgcggcgt gtcaataacg
    32461 ccaaaaattt taccatacag ctctttactt gctttgagct tgcgtatcat cttcggcaca
    32521 aactcgtgcg gaacgtgaca atccggacag gtcgcacgga cgccgctacg gttgttgtag
    32581 tgcacggaat ccatgtattc ctgatacacc gtgttgcgca tttcgtggca gctaatgcag
    32641 aactcttcgg tattggcttt ttccatcccg gtgttaaagc ccccccagaa gacgatgccg
    32701 ccaacaaaac cgatcaacag cagcgtcccc agcgccagat ggctgggggt acgccaccat
    32761 ttccacaggc gcttaatcag accaggctta cggtcagaat ttcccataat aacctcttat
    32821 ttcccgtaac cttttgatgg ggtaaaggta ttccccacga ttggcgcggt atcggcctgc
    32881 ggtacgtgac attgcagaca gaaataacga cgcggagcca cttccgcgcc cactttgccg
    32941 tcgctgtcca taaagtgagt aggactgata cgcggcgcgc cagtggtgcg atagctttcg
    33001 acaccgtggc actgcaagca gcgattggta ttggtcgtta cctgataacc ttcaacgcta
    33061 tgcgggatca ttggcggctg attcacatag ttcagcggca tccgatcctg ctcttttggc
    33121 atccgaatgg ccccttcctg cgtcccggat acttccggcg actggctaaa atcgacgcca
    33181 ttagccgccc aaaccgcgcc gcttaccacc agggccagca tcgccgtcca ttgacacagc
    33241 gctttcttca ggtcatggct tttcatgatt tcgctcccga actccatcgt gtagttattg
    33301 taaatacatc ctcagaacag acatccacgc agcgaccgca cgtcatgcaa tcgcggctgg
    33361 tgacctgcac cgggctttgt tcatccagca ccggggcacg tagcacatgc ggttccgggc
    33421 aaacatgaaa acaatccata cagcggttac atttctgccg atcgctggcc gcaacggtaa
    33481 tcacaccttt gctacccagc acgccataca gcgcgccaac cggacaaatg tgcccgcacc
    33541 agccgtgttc aacgaccagt agatcaaata aaaacagcgc gagaataagc agcgcgccgc
    33601 tgccgaagcc cattaccagg ctacgcccca tcagagaaac ggggttgatc cattcccaaa
    33661 tgagcgtgcc agttaaggct gatcccacca gaatgaccac caacagcacg tagcgaatgt
    33721 ggcgagggat cgtcgcagac tgattgaggt caaagcgcct gcgtaaccag ttcgctaagt
    33781 cggtaatcgg gttcagcggg cagacccagc tgcaaaataa tcgctttccc gccagggcgt
    33841 agagcacggt gataatcacc gcaccggtca acgccaccgt ggcaggcaga tgaccactgg
    33901 caagactttg cagcgtcatc agcggatcgg tcagcggaac ggtgtcgaat aacaggctac
    33961 tgctgtagtt gccgtgcaag atccacacac caaaccatgg accgctcaaa aacatcccca
    34021 gcacgaagaa ctgacaaagg cgacgtaaca ccagccaacg gtgactgcgc caccagcctt
    34081 ttttctccag cgcctcgcgc ccggcgtcac gtttacgatt tgccattgtt cccctccagc
    34141 cagccgaagc ggtaatggtg acctaactcc cctttcgcca gtgacagcgg taacaccttg
    34201 attgccggtt gttccagcac gcatactttt tcgcacttac cgcaaccggt acaggcgtcg
    34261 ctgtgaaccg tcggcagaaa gcgggcgtgc ttaccggtac gcgtgttgcg ctccagctcc
    34321 aaggtgatgg cctcatcaat tttcgggcat tcgcgataac aaacatcgca gcgcagcccc
    34381 tgaaagttga gacagttttc ctggtccacc agtaccgcca gccccatccg cgcgtcgtcg
    34441 atcgattcaa tctcacgatc cagcgcaccg cttgggcaca ctttggcgca cggaatgtcc
    34501 tcacacattt cgcaaggaat atcccgtgcg acaaaatatg gcgtgcccgc cgacagacca
    34561 gaggccagcg tcgccagttt taaggtgtcg taagggcaag cctgaacaca ctgaccgcaa
    34621 cgcacacagg cactggcaaa ggcgttctcg tttatggctc cgggcgggcg caaccgcacg
    34681 ccagatgcgc gtgcggtttg ctgttgtaac cccagcgcca cacccacggc agccagcccg
    34741 cctgctgtgc gaacgacatc gcgcagaaag cggcggcgac cattttgagg tttcgctgac
    34801 cgggacataa tggcttacac cttctccagt ttgaccgcgc acttcttgaa gtccgtctct
    34861 ttcgagagcg gatcggtcgc atccagcgtc agtttgttaa ccagctgtgc ggcgtcgaag
    34921 aacggcatgt acaccaggcc ctgtggcgga cggttacgac cgcgcgtttc aacaatcgag
    34981 atcacttcgc cacggcgaga aaccactttc actttgtcgc cacggcgcag atcgcgcgct
    35041 ttcgcatcca gcgggtgaat aaacaagacc gcttccggga aggcgcggtg cagttccggc
    35101 acacggcgag tcatactgcc ggtgtgccag tgctccagaa cgcgtccggt agagagccac
    35161 aggtcgtact cttcatccgg tgcttccgcc gccggttcga acggcagtgc gaagatcacc
    35221 gctttgccat ccggtttacc gtagaactta tagccttcgc ccgctttcac gtacgggtcg
    35281 ttaccttcgc tgtaacgcca ctgtgtttct ttaccattca ccaccggcca gcgcagaccg
    35341 cgcgctttgt ggtagtcatc gaacggtgcc agatcgtgac cgtgaccgcg accaaaccat
    35401 gcgtactctt cgaacagccc tttttgcaga tagaagccca gctcgcggga ttcatcgttc
    35461 agctgatctt ccgccagttc ggataccggg aatttgctca cttcgggggt ggcatacaga
    35521 acttcgtaca gcgttttgcc acgcagttcc ggtttcttcg ccagcagctc ttccggccat
    35581 acttcttcag ttttgaagcg gcgggagaac tggactaact gccagagatc cgatttcgct
    35641 tcgcctggcg cctgtacctg ctgacgccag aactgagtac ggcgttcggc gttaccgtaa
    35701 gcgccctctt tctctaccca cattgcggtc ggcaggatca agtcggcggc cagcgcactg
    35761 actgtcggat acggatcgga gacgatgatg aagttgcgcg gatcgcgcca gcccggcata
    35821 cgctcttcat tgatgttcgg cccggcctgc atgttgttgg tacacatggt ccagtaaaca
    35881 ttcagcttgc cgtctttcag cgcacggtct tgtgccaccg catgaagacc gattttcgcc
    35941 ggaatggtgc cgctcgggat attccacttc ttctcacaga tatcacgatg tttctcgtta
    36001 gttaccacca tgtccgcagg cagacggtga gcaaaggtgc ccacttcacg cgcagtacca
    36061 cacgcagaag gttgcccggt cagggagaac ggaccgcaac ccggctggga aattttgccg
    36121 gtcagcaggt gcaggttgta gaccaggttg ttggcccaca caccacgggt atgctggttg
    36181 aagcccatcg tccagtagga gatgactttc ttgttcggat cggcatacag ctgcgccagt
    36241 tgttctaact ggtctttcgg cacgccggtc atttcagcgg ttttttccag cgtatactca
    36301 gcaacgaagg ctttgtaatc ttcaaagctc atcggttcgg aggcgtcaga acccggattc
    36361 ttcgctgctt tttccagcgg atgggtcgga cgtaaaccgt agccgatgtc cgtcgcccct
    36421 ttgcgcaggt taacgtgctt gctgaagaag tcctgattta tcgcattgtt ttgaatgata
    36481 tagttggcga tgtagttcag gatcaccagg tcagattgcg gcgtaaagat gatgccgtta
    36541 tccgccagct cgaagctacg atgctggtag gtagaaagca ccgccacggt gacgttctgg
    36601 ttagagagac gacggttagt aatgcgtgac cagaggatcg ggtgcatctc cgccatgtta
    36661 gcgccccaca gcacaaacgc gtcagcctgc tcgatgtcgt catagcagcc catcggctca
    36721 tccataccaa aggtacgcat aaagccaact actgccgacg ccatacagtg acgtgcgttc
    36781 gggtcgatgt tgttcgaacg gaagcccgct ttgaacagct tggacgcggc ataaccttcc
    36841 cagatagtcc actgaccaga accgaacata ccgatcgatt ccggcccttt ttctttcagg
    36901 gcggttttga atttctcttc catcacatcg aaagcctgat cccaggtgat tggggtaaat
    36961 tcgccttctt tgtcatattt accgtttttc atacgcagca gcggctgcgt caaacggtct
    37021 ttaccgtaca tgatcttggg caggaaatag cccttaatgc agttcaggcc acggttaacc
    37081 ggtgcgtccg ggtcgccctg acaggccacc acacgcccct gctgcgttcc gaccagaacg
    37141 ccgcaaccag taccgcagaa acggcacggc g
//
LOCUS       DADLSB010000022        36323 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.022, whole genome shotgun sequence.
ACCESSION   DADLSB010000022 DADLSB010000000
VERSION     DADLSB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.022"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(29..208)
                     /locus_tag="JN331_001101"
     CDS             complement(29..208)
                     /locus_tag="JN331_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY7259299.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(292..1176)
                     /gene="yhdJ"
                     /locus_tag="JN331_001102"
     CDS             complement(292..1176)
                     /gene="yhdJ"
                     /locus_tag="JN331_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7259300.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1262..1558)
                     /gene="fis"
                     /locus_tag="JN331_001103"
     CDS             complement(1262..1558)
                     /gene="fis"
                     /locus_tag="JN331_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY7259301.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1584..2549)
                     /gene="dusB"
                     /locus_tag="JN331_001104"
     CDS             complement(1584..2549)
                     /gene="dusB"
                     /locus_tag="JN331_001104"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY7259302.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2878..3759)
                     /gene="prmA"
                     /locus_tag="JN331_001105"
     CDS             complement(2878..3759)
                     /gene="prmA"
                     /locus_tag="JN331_001105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY7259303.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLYDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRNNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3771..5222)
                     /gene="panF"
                     /locus_tag="JN331_001106"
     CDS             complement(3771..5222)
                     /gene="panF"
                     /locus_tag="JN331_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY7259304.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5212..5454)
                     /locus_tag="JN331_001107"
     CDS             complement(5212..5454)
                     /locus_tag="JN331_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY7259305.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5563..6912)
                     /gene="accC"
                     /locus_tag="JN331_001108"
     CDS             complement(5563..6912)
                     /gene="accC"
                     /locus_tag="JN331_001108"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY7259306.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6923..7393)
                     /gene="accB"
                     /locus_tag="JN331_001109"
                     /gene_synonym="fabE"
     CDS             complement(6923..7393)
                     /gene="accB"
                     /locus_tag="JN331_001109"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY7259307.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8371..9345)
                     /locus_tag="JN331_001110"
     CDS             complement(8371..9345)
                     /locus_tag="JN331_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7259308.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9497..11437
                     /gene="csrD"
                     /locus_tag="JN331_001111"
     CDS             9497..11437
                     /gene="csrD"
                     /locus_tag="JN331_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY7259309.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSIPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNARGQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11742..12785
                     /locus_tag="JN331_001112"
     CDS             11742..12785
                     /locus_tag="JN331_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY7259310.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12851..13954
                     /gene="mreC"
                     /locus_tag="JN331_001113"
     CDS             12851..13954
                     /gene="mreC"
                     /locus_tag="JN331_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY7259311.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13954..14442
                     /gene="mreD"
                     /locus_tag="JN331_001114"
     CDS             13954..14442
                     /gene="mreD"
                     /locus_tag="JN331_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY7259312.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14451..15044
                     /gene="yhdE"
                     /locus_tag="JN331_001115"
     CDS             14451..15044
                     /gene="yhdE"
                     /locus_tag="JN331_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY7259313.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15034..16503
                     /gene="rng"
                     /locus_tag="JN331_001116"
     CDS             15034..16503
                     /gene="rng"
                     /locus_tag="JN331_001116"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY7259314.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16571..20371
                     /locus_tag="JN331_001117"
     CDS             16571..20371
                     /locus_tag="JN331_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY7259315.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20527..21972
                     /gene="tldD"
                     /locus_tag="JN331_001118"
     CDS             20527..21972
                     /gene="tldD"
                     /locus_tag="JN331_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY7259316.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22100..23029)
                     /gene="aaeR"
                     /locus_tag="JN331_001119"
     CDS             complement(22100..23029)
                     /gene="aaeR"
                     /locus_tag="JN331_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY7259317.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23212..23415
                     /locus_tag="JN331_001120"
     CDS             23212..23415
                     /locus_tag="JN331_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY7259318.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23423..24355
                     /gene="aaeA"
                     /locus_tag="JN331_001121"
     CDS             23423..24355
                     /gene="aaeA"
                     /locus_tag="JN331_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY7259319.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24361..26328
                     /gene="aaeB"
                     /locus_tag="JN331_001122"
     CDS             24361..26328
                     /gene="aaeB"
                     /locus_tag="JN331_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY7259320.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPRDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26420..26692
                     /locus_tag="JN331_001123"
     CDS             26420..26692
                     /locus_tag="JN331_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259321.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26748..27011)
                     /gene="yhcN"
                     /locus_tag="JN331_001124"
     CDS             complement(26748..27011)
                     /gene="yhcN"
                     /locus_tag="JN331_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY7259322.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27376..27846)
                     /gene="argR"
                     /locus_tag="JN331_001125"
     CDS             complement(27376..27846)
                     /gene="argR"
                     /locus_tag="JN331_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY7259323.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28281..29219
                     /gene="mdh"
                     /locus_tag="JN331_001126"
     CDS             28281..29219
                     /gene="mdh"
                     /locus_tag="JN331_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY7259324.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29281..30348)
                     /gene="degS"
                     /locus_tag="JN331_001127"
     CDS             complement(29281..30348)
                     /gene="degS"
                     /locus_tag="JN331_001127"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY7259325.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30438..31805)
                     /gene="degQ"
                     /locus_tag="JN331_001128"
     CDS             complement(30438..31805)
                     /gene="degQ"
                     /locus_tag="JN331_001128"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY7259326.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31959..32357)
                     /locus_tag="JN331_001129"
     CDS             complement(31959..32357)
                     /locus_tag="JN331_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY7259327.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32551..33678
                     /locus_tag="JN331_001130"
     CDS             32551..33678
                     /locus_tag="JN331_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY7259328.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33897..34325
                     /gene="rplM"
                     /locus_tag="JN331_001131"
     CDS             33897..34325
                     /gene="rplM"
                     /locus_tag="JN331_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY7259329.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34341..34733
                     /gene="rpsI"
                     /locus_tag="JN331_001132"
     CDS             34341..34733
                     /gene="rpsI"
                     /locus_tag="JN331_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY7259330.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35136..35774
                     /gene="sspA"
                     /locus_tag="JN331_001133"
     CDS             35136..35774
                     /gene="sspA"
                     /locus_tag="JN331_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY7259331.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35780..36277
                     /gene="sspB"
                     /locus_tag="JN331_001134"
     CDS             35780..36277
                     /gene="sspB"
                     /locus_tag="JN331_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY7259332.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
BASE COUNT         8739 a         9055 c         9593 g         8936 t
ORIGIN      
        1 aaacgaatga attaagtgtc atgtaaattc acattctgtc ctgaaaattc agttcgtcac
       61 agttaaccag ttttagtgga ttaactgagt tcatattacg gcatttgtct gtttctgttg
      121 tcagcagttc aatccactgc atcagtaaag tatcaaacag aaaaacgaag acagcgaaaa
      181 cgacgagcca ccaatacttg cgaatcatta cctgagcctg tgcatgttta accaagtctg
      241 gcaggaaata tacacgaaat gaataatctg aaaaccaggc ttacacttcc tttactttgc
      301 aatgagatcg gggtcaactt cgcttaaccg gcttcgtttt gacaggttgc cggtctttct
      361 ttttttcact ttcgccagtt cttccgcgga gtaatgcgac gcgacatcca gccgtcgaag
      421 ccccattttg atgtactcgc tgttgatctc aataccaatg aattttcgtc cgctggcgat
      481 ggctacggca ccggtagtaa agctaccggc aaacgggtcg agaacgatat cgcctgggtt
      541 ggaagaggcg agaataatgc gtttcagtaa ggctttcggt ttttgcgtcg ggtggttctc
      601 atattcatcc atcaaataac gcacgcgcgg aaaatcccag acgttacccg gtactttttg
      661 atgattgtat ggctgtggag gatttttgcg ataatcgatc aacgcgcgct gcgatccggt
      721 tttggcttct accagaatag catcaccgtt gaatgtgtag ttctttgcgt ctttcaccat
      781 catcaggatg ggttcgtaca tggagccgta gtgttttttc gcctgtactc cagaactgtc
      841 atatgaccag acgatgcgac ttttgatggt aaaaagcttg cggcactgga gatcgataaa
      901 gggcatgttt tccgtactgt tcataatgta catgctgccc tgctttttca gaacgcggtg
      961 gcactctgca atcacttcaa acagccagtc gataaacaga tcttctttcc aggcttcgat
     1021 cagaccatca aaatttttac cgatgttata cggtgggtcg gcaaagagca gatcgacgct
     1081 ttcagtaggt agctttttaa gttcggcaag ggcatcaccg tgaataatgg tcttagcttc
     1141 attaccaaac cgggtcggtt cacatcctgt tctcatggtc actccctttg tgacacctat
     1201 aaaaaaggcg cttccccatg ccgagtagcg cctttttaat caagcattta gctaacctga
     1261 attagttcat gccgtatttt ttcaattttt tacgcagcgt accacggttg atgcccatca
     1321 tcagcgcagc acgggtctgg ttaccacggg tgtattgcat caccatgtcc aacaggggct
     1381 gttctacttc agccagtacc agctcataga ggtcattcac atcctgacca ttcagttgag
     1441 caaaatagtt cttcagtgcc tgtttaaccg agtcacgcag gggtttttgg gttacctgat
     1501 cctgagagtt aacggtagaa acggtcagta cgtcagaatt tacgcgttgt tcgaacatag
     1561 ttctgtcagc tctttatttc tgtttacgca aaattttcga agtatgcctc caacgcctcc
     1621 agctgttcgc tggcatcctc aatggcgttg aatgtgcgcc gaaactggtc atttggagcg
     1681 tgttcctgga gataccagga aacgtgttta cgtgcaattc ggtacccttt tgccggacca
     1741 taaaagtcat gcagttcccg aacgtgcgcg caaagcaagc gcttaacctc tgccaaaggc
     1801 agtgggggca gcaactcccc agtgtccaga taatgctgga tttcccgaaa gatccagggt
     1861 cttccctgag ctgcgcggcc tatcatcagg gcatccgccc ctgtatagtc gagcacagct
     1921 ctggctttaa gcgggtcagt aatgtcgcca ttcgcgataa ccggaatgga aactttctgc
     1981 ttaactgccc gaatactgtc gtactcagct tctccattga acaaacaggc gcgtgtacgg
     2041 ccatgaatgg tcagagcctg aatgccacag tcttcagcca gttgggcaat ctcttcgcag
     2101 ttacggtgtt ccggtgccca gccggtgcga atcttcaggg taacaggaac gtccactgca
     2161 ttgacgacct cggtaaggat cgatttaacg acatccgggt actgcaagag ggctgaacct
     2221 gcgagcttgc gattcacttt tttagccggg caacccatat tgatatcaat aatctgggca
     2281 ccgctttcca cgttaatacg tgctgcatct gccatttctt tcggagcgct accagcaatt
     2341 tgcacggtgc gaataccggg ttcatcaatg tgcaccatcc gtaaacgaga tttgtcgctt
     2401 tcccaaacct gtgggttaga agacatcatc tcggatactg tcaatccggc tcccatctcg
     2461 tagcacaacg tccgaaaagg tctgtctgta atgccagcca tgggcgctgc gatcaggcga
     2521 tttctgagct gatattgtcc gatgcgcatg agttaagaaa tgaccatact gtgactgcaa
     2581 ggcggcgtat attacgcatt ttttgcacga gatgaaaggc caaactttga ccaatcctct
     2641 gagatggatc aaagaattgc atttaaaatg agcgtggtgc gataattact cataaaaatc
     2701 atcatattag aaaatagtga ctaaaattta cactcaaaga aatttgagta agttctcaat
     2761 ttttctttat gaatgaaaat tttggcacgc aaatttgcgt aaataatcgg caaatttacg
     2821 tgccttttgt gagcttgctc gcacttcgcc ccgcgtcacc ctacgccgat gcgaaggtta
     2881 attcttacga ccggtaatac ggcaccactc ttctttttcc acgaccgggt ccagtgcgaa
     2941 gctatcggca taagcttcac aaacgctctc tgcctggctt gccagaatac cggaaaggcc
     3001 cagcaaaccg cctgaaaccg gcaggacgct gattaacggt gccagttcac gtaatgggcc
     3061 tgcaaggatg ttagcgacca ccacgtcggc tttcatttct tctggctgat ctttcggtaa
     3121 gtagagttcc agacggtcag aaacgccatt acgttcggcg ttattgcggc tggcctgaat
     3181 cgcctgcgga tcgatatcaa taccaatggc ttttgctgca cccagtttca gcgccgcgat
     3241 cgccagaatg ccggaaccac agccaaagtc gatgactgtt ttaccggtta aatcgaggct
     3301 gtcgagccat tgcaggcaca gagaggtggt tggatgggta cccgtaccaa acgccagccc
     3361 tggatctaac atcacgttga cggcgttttc gtccggcaca tcacgccagc tagggcagat
     3421 ccacagtcgt tcaccaaagc gcatcgggtg gaaattatcc atccattcgc gctcccagtc
     3481 tttatcttct agttgttcga ttttatgcgc gaagcctgcg ccgagcagcg gatggttttc
     3541 cagaatcgcc accacgtcgt tcatatcggt ttcagcgtcg tacagaccaa tcacatcggt
     3601 gtcgccccac aggcgcgttt cgcccggcag cggctcaaat actggcgtat cgtgggtatc
     3661 ctgaaaagtg atagaaacgg cacccgcttc catcagcgca tcgctaagat cttccgcgtt
     3721 cgcgccggtg gtgttcagtt tcagttggat ccaaggcatg gcaaaactct ttatttatca
     3781 gtagtcaaaa cggtagcttg tgggacggat gtaccgaaac ggtttccgac caggaaagcc
     3841 agcaaactta gtagtaacga gggcacgata gggtggaagc ccaggtactg aatattcagc
     3901 gtcgcgagta cggcatacag cacgccgcca acgatcatcg cacttagcgc gcctttggcg
     3961 ttggcgcgtt cccagtaaag acccagcacc agcggccaca ggaaaacggc ttccagccca
     4021 ccgaaggcca gcaaattcag ccagatgatc atttctggcg gcttccaggc ggcaagcagc
     4081 agcaacgcgc cgagaactaa cgtaattacc gccgacatcc gcttcagacg cgtctcgttt
     4141 tgcatttgat ccggacggat attcagatag agatctttaa tgatcgtagc ggaactttgc
     4201 agcagttggg cgttaattgt cgacatgatc gcagccatag gtgcagccag aaagatcccg
     4261 gcagcaaaag gtggcagcac ttttaccatt aacgtcggga tcaccagatc cggtacggtg
     4321 agatcgggga tcactgcacg acctaacgct ccggccaggt gcataccgaa catcaggatt
     4381 gcgactacaa tcgtaccgat gatgatcccc cggtgtactg ctttgctgtc tttataagag
     4441 atacagcgca ccgcagtatg cggcaggcca atcacgccaa agcacaccag tacccagaac
     4501 gacgtcataa aggcaggcga cagaatatcg tcagcgcctt gtggcgtaac cagttgcgga
     4561 tcgatggttt gcaaggtctg tactgcgttg cttaagccgc cagcggcatg taccacgcca
     4621 ataagcagca caacggtgcc aatcagcatc acaagccctt gcatggtgtc gttcagcacg
     4681 ctggcgcgaa agccaccaaa ggcggtatat aacgcaatgc tgataccaaa aatcagcagc
     4741 ccggtttcat aaggaatacc cgccgcggtt tccagcaggc gcgcaccgcc gataaactgc
     4801 acggtcatcg caccaacgaa cgcaaccagc aaactcaaac tcgccagcca caccagaaga
     4861 cgactctggt agcgggcaaa cagcatatcg ttcagcgtca ctgcattgta gcggcgcgca
     4921 agaatcgcaa acttcttgcc gagaataccg agtgaaagcc agacagcagg aagctgaatc
     4981 atcgccagca atacccagcc cagcccgtat ttataagcag ctcctggccc gccgataaac
     5041 gaactggcac tgatataggt cgcggtgagc gtcatcgcca gcacaatacc gcccatagag
     5101 cggctgccga ggaaatactc attaaggaag gtgccggtgc tccgtttacg catcgcataa
     5161 accgagatac cgaacaccac caccagatag gcgaccagcg gtagaattac ttcaagctgc
     5221 atcgtcatcc tccagtggga tatcgcgata gataaatttc accatcgccc agcacagtcc
     5281 aataaacagt agcggcgtca ggatgcaggc catctcaaac cagcgcggaa agccggtaaa
     5341 accgggggca acgccaggta agtaagcgtc tactaaccaa actgccagat acaaaagggt
     5401 cagccccagc gcccagcgcg cctctttatg ggcctgaaca aaacgagtgt ccattttttg
     5461 tccctgatgg gtgaagaaag cgaggattgt accttatggg ggttgtcgat ccccagtaat
     5521 aaaaaaggcc ggaaaatccg gccttttgac gcttttgcag tcttattttt cctgaagacc
     5581 gagttttttc tccagatagt ggatgttagt gccaccatgc tggaagttct cgtcattcat
     5641 gatgcggatc tgcagatcaa cgttggtttt gataccgtcg atgatcagct cctgcagcgc
     5701 attcttcatg cgggcaatcg ccacgtcacg gttttcaccg tagcaaatca gcttaccgat
     5761 cattgagtca tagtacggcg gtacggtgta gcccgcgtag atatgagact cccaacgtac
     5821 gccaaaaccg ccaggtgcgt ggaaacgggt gattttgcct gggcttggca ggaaggtgtt
     5881 cggatcttcg gcgttgatac gacattccac cgcatggccg cgaacgtgaa cttcttcttg
     5941 cttgatcgac agcggttgac cggcagcgat acgcagctgt tctttgatca ggtcaacgcc
     6001 ggtgatcatt tctgtaaccg ggtgttctac ctgaatacgg gtgttcattt cgatgaaata
     6061 gaactcgccg ttttcgaaca ggaactcgaa agtacctgca ccgcgatagc cgatatcaac
     6121 acacgcttta gcgcaacgtt cgccgatgta gcgacgcagt tccggggtaa tgcccggtgc
     6181 tggtgcttct tcgaccactt tctggtggcg gcgctgcatg gagcagtcac gttccgccag
     6241 atagatagcg ttgccctgac cgtcagccag tacctgaatc tcgacgtggc gaggattttc
     6301 caggtatttc tccatgtaaa ccatatcgtt gctgaaagca gctttcgctt ccgcacgggt
     6361 catggagatg gattgtgcca gttcagcgtc gccgcgcact acgcgcatac cgcgaccgcc
     6421 gccgccgccg gaggctttga taatcaccgg ataaccaatg cgtttagcaa tggcgcggtt
     6481 tttatccata tcgtcgccca gcgggccgtc agaaccaggt acgcaaggaa cgcccgcttt
     6541 tttcatcgcc gcgattgcgg atactttgtc gcccatcagg cgaatggttt ctgctttcgg
     6601 gccgatgaag ataaagccgg agcgttcaac ctgctcggca aagttggcgt tctcggagag
     6661 gaagccgtaa cccggatgga ttgctactgc gccggtgatt tcagcggcgc tgatgattgc
     6721 cgggatgttc agataacttt ttactgacgg agcagggcca atacagaccg tttcatctgc
     6781 cagtaatacg tgttttagat cgcgatccgc gctggagtgc acagcgacag tcttgatgcc
     6841 cagttcttta caggcacgaa gaatacgcaa tgcaatctcg ccgcggttgg caataacaat
     6901 tttatccagc atgttcgcct cgttactcga tgacgaccag cggctcgtca aattctaccg
     6961 gttgtccact ttcgaccaga attgctttca cggtaccgga tttgtccgct tcgatctggt
     7021 tcatcatttt catggcttca acgatgcaca gggtatcgcc cacgttgact ttctgaccca
     7081 cttcgatgaa cgcttttgcg tccgggcttg gggtgcggta gaaagtacca accatcgggg
     7141 aacgtacgat gtgaccactg atttccgctg ctgctggcgc ttccatggaa ggaacggtcg
     7201 ccggagcggc tgcgttagat tgagctggct gctgcatcat tggtgcagcg taagcttgtt
     7261 gcatcacagg gaaacttgcg gcaggagctg cacggctaat gcgtactgac tcttcgcctt
     7321 cagaaatttc cagttcggag atgcctgatt cttcaaccag ctcgatcagt tttttaatct
     7381 tacgaatatc catgagtggg ttccgtactc tttgtttagt gtgattgtga caggcgtttc
     7441 accgccgtct gtaaagcgta tgccccgaca gctgtatgca tagcgataaa ttccagcagg
     7501 ccgggacgcc ggtctatttt gccccagagt gtcaatgtta gacttgacgg acattgtgca
     7561 gcgatctgct gctatcctcc ggcaaaaaac aaaatatacc ttcgttgcgc caatgtcacc
     7621 ttttccgcag cgcaaaaact gcgtcaggga ggacgaggtc gcacattata acgatttcgt
     7681 agcaattggc agctaaatac tggtcttatc agggaagata atcaacagct aacatgtaaa
     7741 taaccttcaa caccgtgtaa tttgtaacaa gccgcacaat tcacgaaatt agcgccatca
     7801 tcgacggaac ttcttataac gtaaggtgaa aagctgaaaa gccagccctg cgaagatgag
     7861 gggctgcaat gagataatct tcacagacca caaataatgt agggatgcca ggatcgcgac
     7921 aagatagacg acattacgca gcggttgctg gggattaccc aattttcact gcatcgtctg
     7981 gatagatgtg aatgctaaag cacgcaaaat caaccaactg acaattctca gcattaaata
     8041 ggttcgagtg gttaatttgt agtcaaataa aatcagatta ttcaccccat ggtaccagaa
     8101 atcagtaaca ggttaagcgt agtgtcgccc aggcaaagca ccataaacct aacaggcgac
     8161 ggaggcgtat caataatggc tgtttagagg agtatcaatg cgcatcaatt tgtggtcagt
     8221 cgaaatcatg actttccagt ttggatgatg ttgtacaact tcctgaccaa ggtgttcgac
     8281 aaaagctgtc cggctaaccg cttttcccgt taaccgattg aataatgatg tcaaaatgag
     8341 ttccctctct tattattcct tgctaaatgg ttagttaacc ttcaccagcg tgcgaccctg
     8401 gatctggtta ttaatgatgg cctcggcaaa gttcggtgcc tctgacagag atatctcttt
     8461 tgccgcctgg gtatagaatg attccggtaa atcggcgacc agtcgctgcc aagcttgtgc
     8521 gcggcgttct ggtggcgtca ttactgaatc caccccttgc aaacggacat tacgcagaat
     8581 aaatggcatg accgtggttg gcagagtaaa accacccgcc agaccacagg ccgccacgca
     8641 gccgccgtaa ttcatttgcg ctagcacttt tgccagcact ttgtcgccaa cagtgtcaat
     8701 tgccccagcc cagacttgtt tttccagagg acgggattcg gcaaactcat cacgagggag
     8761 aatacggctg gcacctaaac ttttcagata ttcatgggta ctttcgcgac cggaaacggc
     8821 aacgacctga taacccaact tatgcagcag cgccacggcg gtactgccga cgccaccact
     8881 ggcacccgtc acgacaatct ccccgtcctg cgggcgaaca ccggcatctt ccagcgccat
     8941 cacacacagc atggcggtaa aaccggcagt accgataatc attgctttac gcgcgtccag
     9001 cccttgcggc atggcaacca gccagtcacc tttcactcgc gcctgctccg ccagcccacc
     9061 ccagtggttt tcaccaacgc cccagccagt gagtaacacc tcctgaccgg catgaaaacg
     9121 cggatcttcg ctggtgcgta cagttccggc aaaatcgatc ccaggaatca tcggaaaatt
     9181 acggatgatt tttcccttac cggtaatcgc cagcgcatct ttatagttca ggctcgacca
     9241 gtgaacatcg accgtgacat cgccctccgg caggcgactt tcgtccagag tctgtactga
     9301 tgcgagagtt ttgccgtcct gctgttctaa aagtaacgcc tgcataagtg gtcctcatgt
     9361 gcatgatgga ttggaaaata atttctgaag actatactcg ctaatggaaa agcaaagccg
     9421 atgaagcgca ataaattgcg agataaatct gatttgctag tatgcccgct tcctcactat
     9481 cggagttaac acaaggatga gattaacgac gaaattttcg gcctttgtta cgctgctcac
     9541 cgggttaaca atttttgtga ctttgctggg ctgttcgcta agtttctaca acgccattca
     9601 gtataagttt agtcatcgcg ttcaggcggt ggcgacggcg attgataccc accttgtgtc
     9661 gaatgacttc agcgcattaa ggccacaaat taccgaatta atggtgtcag cagatatcgt
     9721 tcgtgtagac ctgctgcatg gtgataaaca ggtttatacc ctggccagaa atggtagtta
     9781 tcgtccggtt ggcaccagcg atctgtttcg tgaactgagc attccgttga taaagcatcc
     9841 ggggatgtcg ttgcgtctgg tttatcagga tccgatgggc aactatttcc attcgttgat
     9901 gaccaccgcg ccgctcacgg gggcgattgg ctttatcatt cttatgctct ttctggcggt
     9961 acgctggtta caacggcaac ttgccgggca agaattgctg gaaacccggg ctactcgtat
    10021 cttaaacggc gagcgtggct ctaatgtgtt gggaaccatc tatgaatggc cgcccagaac
    10081 cagcagtgcg ctggatacgc tgcttcgtga aattcagaac gcacgcggac aacacagccg
    10141 tcttgatacg ctgatccgct cttatgccgc acaggacgtg aaaaccggcc tcaataaccg
    10201 actctttttc gataatcagt tagcaacgtt actggaagat caggagaaag taggtaccca
    10261 cgggatcgtg atgatgattc gtctgccgga tttcaatatg ttgagcgata cctgggggca
    10321 cagccaggtt gaagaacagt tcttcactct gacgaatctg ctgtcgacat ttatgatgcg
    10381 ctaccctggc gcactgctgg cgcgttacca ccgcagtgat tttgctgcgc tgttaccgca
    10441 ccggacgtta aaagaggcag agagcatagc cagtcagtta atcaaagccg tcgatacctt
    10501 gccgaacaat aaaatgctcg atcgcgacga tatgatccac attggtatct gtgcctggcg
    10561 tagtggtcag gataccgagc aggtaatgga acatgcagag tctgccacgc gtaatgcggg
    10621 attgcagggt ggcaatagct gggctattta tgatgactcg ttgcctgaaa aaggacgcgg
    10681 taatgttcgc tggcgtacgc ttatcgagca aatgctcagt cgcggcggcc cgcgccttta
    10741 tcaaaaaccg gcggttactc gcgaaggtca ggttcatcat cgcgaactca tgtgccgcat
    10801 cttcgatggt aatgaagagg ttagctcggc ggagtatatg ccgatggtct tgcagtttgg
    10861 cttatcggaa gagtatgacc gtctgcaaat cagccgtctt attccactat tgcgttactg
    10921 gccagaggaa aatctggcga ttcaggttac cgttgagtcg ctgattcgtc cgcgttttca
    10981 gcgttggctg cgcgatacgt taatgcaatg tgaaaaatca caacgaaaac gcataattat
    11041 tgaacttgca gaggccgatg taggtcaaca tatcagtcgt ttacaacctg ttattcgttt
    11101 agtgaatgct ttaggggtac gggtagccgt caaccaggct ggtttgacgc tggtaagcac
    11161 cagttggatc aaagaactta atgttgagtt actcaagctc catccggggc tggtcaggaa
    11221 cattgagaag cgaacggaga accagctgct ggttcaaagc ctggtggaag cctgctccgg
    11281 gaccagcacc cacgtttacg ccaccggcgt gcgttcgcga agcgagtggc agaccctgat
    11341 tcagcgcggt gttacaggcg ggcaagggga ttttttcgcg tcctcacagc cacttgatac
    11401 taacgtgaaa aaatattcac aaagatactc ggtttaacct gccgtttaat ccgttttcac
    11461 gtagaataac gcgcgctgcg tctcatggga gtgtgcttgt ctgctcgcca gattgttgca
    11521 gcacatatgc agatgaatga ccttacgcgg ttgcaaacag gcgaggaatg ctgctgatgc
    11581 attaagcctt tctggactca ggcagagatt tgtaacaaag gaaacgaact gcactaattt
    11641 tcaccgtagc agatgatttt tgcgccttgt cgctgctgcg tgtggttggt aaagtaagcg
    11701 gattttcttt tccgccccag ctttcaggat tatcccttag tatgttgaaa aaatttcgtg
    11761 gcatgttttc caatgacttg tccattgacc tgggtactgc gaataccctc atttatgtaa
    11821 aaggacaagg catcgtattg aatgagcctt ccgtggtggc cattcgtcag gatcgtgccg
    11881 gttcaccgaa aagcgtagct gcagtaggtc atgacgcgaa gcagatgctg ggccgtacgc
    11941 cgggcaatat tgctgccatt cgcccaatga aagacggcgt tatcgccgac ttcttcgtga
    12001 ctgaaaaaat gctccagcac ttcatcaaac aagtgcacag caacagcttt atgcgtccaa
    12061 gcccgcgcgt tctggtttgt gtaccggttg gcgcgaccca ggttgaacgc cgcgcaattc
    12121 gtgaatccgc gcagggcgct ggtgcccgtg aagtcttcct gattgaagaa ccgatggctg
    12181 ccgcaattgg tgctggcctg ccggtttctg aagcgaccgg ttctatggtg gttgatatcg
    12241 gtggtggtac cactgaagtt gctgttatct ccttgaacgg cgtggtttac tcctcttccg
    12301 tgcgcattgg tggtgatcgt ttcgacgaag ctatcatcaa ctatgtgcgt cgtaattacg
    12361 gttctctgat cggtgaagca accgcagaac gtatcaagca cgaaatcggt tcggcttacc
    12421 cgggcgatga agtccgtgaa atcgaagttc gtggccgtaa cctggcagaa ggtgttccac
    12481 gcggttttac cctgaactcc aacgaaatcc tcgaagcact gcaggaaccg ctgaccggta
    12541 ttgtgagcgc ggtaatggtt gcactggaac agtgcccgcc ggaactggct tccgacatct
    12601 ccgagcgcgg catggtgctc accggtggtg gcgcactgct gcgtaacctt gaccgtttgt
    12661 taatggaaga aaccggcatt ccagtcgttg ttgctgaaga cccgctgacc tgtgtggcgc
    12721 gcggtggcgg caaagcgctg gaaatgatcg acatgcacgg cggcgacctg ttcagcgaag
    12781 agtaatcgga tgcaggcagg ggaagcgtct gtttaccctg cctggtctga tacgagaata
    12841 cgcataactt atgaagccaa tttttagccg tggcccgtcg ctacagattc gccttattct
    12901 ggcggtgctg gtggcgctcg gcattattat tgccgacagc cgcctgggga cgttcagtca
    12961 aatccgtact tatatggata ccgccgtcag tcctttctac tttgtttcca atgctcctcg
    13021 tgaattgctg gatggcatat cgcagacgct ggcctcgcgt gaccaactag aacttgaaaa
    13081 ccgggcgtta cgtcaggaac tgttgctgaa aaacagtgaa ctgctgatgc ttggacaata
    13141 caaacaggag aacgcgcgtc tgcgcgagct gctgggttcc ccgctgcgtc aggatgagca
    13201 gaaaatggtg actcaggtta tctccacggt taacgatcct tatagcgatc aagttgttat
    13261 cgataaaggt agcgttaatg gcgtttatga aggccagccg gtcatcagcg acaaaggtgt
    13321 tgttggtcag gtggtggccg tcgctaaact gaccagtcgc gtgctgctga tttgtgatgc
    13381 gacccacgcg ctgccaatcc aggtgctgcg caacgatatc cgcgtaattg cagccggtaa
    13441 cggttgtacg gatgatttgc agcttgagca tctgccagcg aatacggata ttcgtgttgg
    13501 tgatgtgctg gtgacctccg gtctgggcgg tcgtttcccg gaaggctatc cggtcgcggt
    13561 tgtctcttcc gtaaaactcg atacccagcg cgcttatact gtgatccagg cgcgtccgac
    13621 tgcaggactg caacgtttgc gttatctgct gctgctgtgg ggcgcggatc gtaacggcgc
    13681 taacccgatg acgccggaag aggtgcatcg tgttgctaat gaacgtctga tgcagatgat
    13741 gccgcaggta ttgccttcgc cagacgcgat ggggccaaag ttacctgaac cggcaacggg
    13801 gatcgctcag ccgactccgc agcaaccggc gacaggaaat gcagctactg cgcctgttgc
    13861 gccgacacag cccgctgcta atcgctctcc acaaagggct acgccgccgc aaagtggtgc
    13921 tcaaccgcct gcgcgtgcgc cgggagggca atagtggcga gctatcgtag ccagggacgc
    13981 tgggtaatct ggctctcttt cctcattgcg ctgttgctgc aaatcatgcc ctggccggat
    14041 aacctgattg ttttccggcc aaactgggtg ttacttatct tgttgtattg gatcctggcc
    14101 ttgccccatc gcgtaaatgt gggcacaggt tttgtgatgg gtgccatact ggatctgatc
    14161 agcggctcga tgcttggcgt acgcgtattg gcgatgagca tcattgctta cctggtggcg
    14221 ctgaaatacc agcttttccg caacctcgca ttatggcagc aggcgctggt cgtcatgttg
    14281 ctttcgctgg tggtggatat tattgttttc tgggcagagt ttttagtgat taacgtctct
    14341 ttcagaccgg aagtgttctg gagtagtgta gtcaatgggg tgctctggcc gtggattttc
    14401 ttgctgatgc gtaaagtccg tcagcagttt gcagtgcaat aaaggtttct atgacttctc
    14461 tgtatttagc ttccggttct ccgcgtcgtc aggagttact tgcgcaactt ggcgtgacct
    14521 ttgaacgtat tgttacgggc attgaggagc agcgtcagcc gcaggagagc gcgcagcagt
    14581 atgttgtgcg tctggcacgc gagaaagcac aggcgggtgt cgcgcaaacg gcgcaggatc
    14641 tcccggtgct gggtgcggat attatcgtta tcctgaacgg agaagtgctc gagaaaccgc
    14701 gcgacgcaga gcatgcggcg cagatgttgc gcaaattatc gggtcagacc catcaggtga
    14761 tgacggcagt ggcgttggcc gacagccagc acattctcga ttgcctggtg gtcaccgatg
    14821 tgactttcag aacgttaaca gacgaagaca tcgcgggcta tgtcgccagc ggtgaaccgt
    14881 tagataaagc aggtgcatac ggtattcagg ggctgggtgg ctgttttgtc aggaagataa
    14941 atggcagcta tcacgccgta gtcggcttac cgctggttga aacgtatgaa ttattaagta
    15001 attttaacgc actgcgtgag aaaagggata aacatgacgg ctgaattgtt agtaaacgta
    15061 acgccttcgg aaacgcgagt ggcgtatatt gatggcggta ttctgcagga aattcatatt
    15121 gaacgtgagg cgcgacgcgg aatagtaggc aatatctaca agggtcgtgt aagtcgtgta
    15181 cttccgggta tgcaggcggc ttttgtagat attgggctgg ataaagccgc gtttcttcat
    15241 gcatccgaca tcatgccgca taccgaatgt gtggcgggtg aagaacaaaa gcaattcacg
    15301 gtgcgcgaca tctcggaact ggttcgtcag gggcaagatc tgatggtgca ggtggtgaaa
    15361 gatccgcttg gcactaaagg tgcgcgcctg accaccgata tcacgctgcc ttctcgctat
    15421 ctggtattta tgccaggggc ttctcacgtt ggggtttccc aacgaattga aagcgaatca
    15481 gaacgtgaac gcctgaaaaa agtggtcgca gagtattgcg acgagcaggg cgggtttatc
    15541 atccgcaccg cagcggaagg ggttggcgag gctgaactgg cctccgatgc cgcttatctg
    15601 aaacgcgtct ggaccaaagt tatggagcgt aaaaagcgcc cgcagacccg ttatcagctg
    15661 tacggcgaac tggcgctggc gcagcgtgtt ctgcgtgatt ttgccgacgc cgaactggac
    15721 cgcattcgcg ttgactcacg cctgacttac gaagcattgc tggagtttac ctcggagtac
    15781 attcccgaga tgaccagcaa gctggagcat tacaccggac gccagccgat tttcgatctc
    15841 tttgatgtcg aaaacgaaat ccagcgagcg ttggaacgca aagtagaact gaaatccggt
    15901 ggttatctga ttatcgacca gaccgaagcg atgaccaccg tggacatcaa taccggagcg
    15961 tttgtcggtc atcgcaatct ggacgacact attttcaata ccaatattga agcgacgcag
    16021 gctatcgctc gccagttacg gttgcgtaat ctgggcggga ttatcattat tgatttcatc
    16081 gatatgaata atgaagatca ccgccgccgc gtgctgcatt cgctggagca ggcgttgagc
    16141 aaagaccggg tgaaaaccag cgttaatggt ttttcggcgc tggggctggt ggagatgacg
    16201 cgtaaacgca cccgcgaaag cattgagcac gtactgtgta acgaatgccc aacctgccac
    16261 ggtcgcggaa cggtgaaaac cgtggaaacg gtatgctatg aaatcatgcg cgagattgtt
    16321 cgtgtccacc atgcttacga ctccgaccgt ttcctggtct atgcttctcc ggcagtagcc
    16381 gaagccttga aaggcgaaga gtcacactcg ctggcggaag tggaaatttt cgttggcaaa
    16441 caggttaaag tacaaattga accgctctat aaccaggagc agtttgacgt cgtaatgatg
    16501 taaacagatg ctgggccgcc atccggcaaa gggtttttga gtcacatttt tagcagacaa
    16561 ggagtgacgg gtgaggcgat tgccggggat tttactgctt actggagccg cgctcgttgt
    16621 gatcgctgcc ctgctggtta gcggcctgcg tattgcttta ccgcatcttg acgcctggcg
    16681 tccggaaatc ctcaacaaaa tagaatccgc gactggcatg ccggtagaag ccagtcagct
    16741 ctcagccagc tggcagaatt ttggcccgac gcttgaagca cacgacatcc gtgcagaact
    16801 aaaagatggc ggcgaatttt cggttaaacg cgttactctg gcgctggatg tctggcagag
    16861 cctgttacat atgcgctggc agtttcgcga cctcactttc tggcagctgc gctttcgcac
    16921 caacactcct atcaccagcg gtggtagcga tgacagtctg gaagccagtc acatcagcga
    16981 tctgtttctt cgtcaatttg accatttcga tctccgcgac agtgaagtca gttttctgac
    17041 gccatccggt cagcgcgccg agctggcgat cccacaactc acctggctga acgatccacg
    17101 tcgacaccgt gcggaaggcc tggtaagcct ctccagcctt accggacagc acggcgtgat
    17161 gcaggtgcgc atggatttgc gcgatgatga ggggttgtta agcaatggtc gcgtctggct
    17221 acaggcggat gacatcgacc tgaagccgtg gctcggtaaa tggatgcagg acaatattgc
    17281 tctggaaacg gcacagttct cccttgaagg ctggatgacg atcgacaaag gcgatgtaac
    17341 cggcggtgac gtctggctga aacagggcgg tgccagctgg ttgggcgaga agcaaacgca
    17401 tacgctgtcg gtggataatc tgaccgcgca tattacgcgt gaaaatccgg gctggcagtt
    17461 ctctattccc gatacacgga tcacgatgga cggcaaaccc tggccgagcg gagcattgac
    17521 gctggcctgg ataccggaac aggacgttgg cggcaaagac aataaacgca gtgacgaact
    17581 ccggattcgc gctagtaatc tggagctggc aggcctggag ggcatacgcc cgctggccgc
    17641 gaaactttca cctgcactgg gtgatgtttg gcgctccaca caaccgagcg gcaagattaa
    17701 cactctggcg ctggatatcc cgcttcaggc ggcagacaag acccgttttc aggcatcgtg
    17761 gagcgatctg gcctggaagc aatggaaatt attaccgggt gcggaacact tctccgggac
    17821 gctttccggc agcgttgaaa atggtttgct taccgcgtcg atgaagcagg caaagatgcc
    17881 ttacgaaacg gtattccgtg cgccactgga aatcgccgac ggccaggcaa ctataagctg
    17941 gctgaacaat gacaaaggtt tccagctgga tgggcgtaat attgacgtta aagccaaagc
    18001 cgtccatgcg cgcggcggtt ttcgttacct gcaacctgct aacgatgaac cctggctggg
    18061 tattctggct ggcatcagta ccgatgatgg ttcacaagcc tggcgctatt tcccggaaaa
    18121 cttgatgggt aaagacctgg ttgattattt aagtggtgcg attcagggcg gtgaagcgga
    18181 taacgcgacg ctggtttatg gtggcaatcc gcaactcttc ccctataaac acaacgaagg
    18241 tcagtttgaa gtgctggtgc cgctgcgcaa cgcgaagttt gccttccagc cggactggcc
    18301 tgcattaact aaccttgata ttgaactgga ctttattaac gacggtttat ggatgaaaac
    18361 cgatggcgtt aatctgggcg gcgtgcgcgc gagtaatctc accgcagtga tccctgacta
    18421 ctcaaaagaa aaactgctga ttgacgctga cattaaaggt ccgggtaaag ccgttggccc
    18481 ttactttgat gagacaccgc tgaaagattc tctgggtgcg accctgcaag aactccagct
    18541 cgacggcgat gtgaatgctc gcttacatct tgatatcccg ctgaacggcg aactggtaac
    18601 cgcgaaaggt gaagtgacgc tgcgtaataa cagtctgttt atcaaaccac tcgacagcac
    18661 cctgaaaaat ttgagcggta aattcagctt tatcaatggc gatctgcaaa gtgaaccact
    18721 gacagcaagc tggtttaatc agccgttgaa cgtggatttt tccaccaaag aaggggcaaa
    18781 agcctaccag gttgcggtga atctcaacgg taactggcaa ccggcgaaaa ccggcgttct
    18841 gcctgaagcg gtgaacgaag cattgagtgg cagcgtggcg tgggatggta aagtgggcat
    18901 tgatctgcct tatcatgctg gcgcgaccta taacgtagag ctgaacggcg atctgaataa
    18961 tgtgagtagt cacttacctt caccgttagc caaacctgcg ggtgaaccac tagcggtaaa
    19021 cgttaaggtt gatggcaatc tcaacagctt tgaattaacc ggacaggctg gtgcggataa
    19081 ccatttcaat agccgctggt tgcttggtca aaagctgacg ctcgatcgtg ctatttgggc
    19141 ggcagacagt aaaacgctcc cgccgttgcc ggaacaaagt ggcgttgaac ttaatatgcc
    19201 gccgatgaat ggtgccgagt ggctggccct gttccagaaa ggcgctgcgg agagtgtcgg
    19261 tggtgcagcg agtttcccac aacacataac gttacgtacg cctatgttgt cactgggaaa
    19321 tcagcaatgg aataacctga gtattgtttc gcaaccgacg gcaaatggca ccctggttga
    19381 ggcgcaaggg cgtgaaatca acgccacgct agcgatgcgt aataacgcgc cgtggctggc
    19441 gaatatcaaa tatctttatt acaacccgag cgtggcgaaa actcgtggtg attcaacgcc
    19501 gtcatcacct ttcccgacaa cggagcgcat taacttccgt ggctggccgg acgcacaaat
    19561 acgatgcaca gagtgctggt tctgggggca aaaattcggt cgcattgaca gtgatatcac
    19621 catttctggc gatacgttaa cgctgaccaa tggactgatt gatactggtt tctcgcggct
    19681 tactgccgat ggtgaatggg ttaataatcc ggggaatgaa cgtacctcgc tgaaaggaaa
    19741 actgcgtggg cagaaaattg atgccgccgc agaatttttt ggtgtcacga cgcccatacg
    19801 ccagtcgtca tttaatgtgg attacgattt acactggcgc aaagcaccct ggcagccaga
    19861 tgaagcgacg ttgaatggca tcattcatac tcaactgggt aaaggcgaaa ttaccgaaat
    19921 caataccgga catgccgggc aattgctgcg cttattgagc gtagatgccc tgatgcgtaa
    19981 gctgcgtttt gatttcagag acacttttgg cgaagggttc tattttgact ccattcgcag
    20041 caccgcgtgg attaaagacg gcgttatgca caccgacgac acgctggtgg atggcctgga
    20101 ggcggatatc gccatgaaag ggtcggtaaa tctggtacgt cgcgacctga atatggaagc
    20161 ggttgtcgca ccagagattt ctgcgacggt gggcgtggct gcggcttttg cggttaaccc
    20221 cattgttggc gcggcagtgt ttgccgccag taaagtgctg gggccgctgt ggagcaaagt
    20281 ctccattttg cgctatcaca tttcgggtcc gctggacgat ccgcaaatca acgaagtgtt
    20341 gcgccaaccg cgtaaagaaa aagcgcaatg atttgacgag ggcgcgtaat tgccccaatc
    20401 tcatatgata atcgttgcca aaggccaacg agccggaaca taactgtagg ccggataggg
    20461 cgttcacgcc gcatccggca gccgtaaaaa atcctctact gcagtaacta acgagtagca
    20521 aaaacgatga gtctgaacct ggtaagtgaa caattgctag cggcgaacgg cctgaaacat
    20581 caggacttgt tcgcgatcct cggtcaactg gccgaacgtc gccttgatta tggcgatctc
    20641 tattttcagt cgagctatca cgaatcctgg gttttagaag accgcattat taaagatggt
    20701 tcttacaaca tcgatcaggg cgttggcgtg cgtgcaatca gcggtgaaaa aaccggattt
    20761 gcttacgctg accaaatcag cctgctggcg ctggaacaga gtgcgcaagc ggcgcgcacc
    20821 atcgtccgtg atagtggtga tggcaaagta cagacgctgg gcgcggtaga gcatagcccg
    20881 ttgtatacct cggtagatcc gctgcaaagc atgagccgtg aagagaagct ggatatcctg
    20941 cgtcgcgtcg ataaggttgc tcgcgaagcg gacaagcgcg tacaggaagt gactgccagc
    21001 ctcagtggtg tctatgaatt aattctggtt gcggccaccg acggcacgct agcggcggat
    21061 gtccgtccac tggtgcgtct ttccgtgagc gttctggtcg aagaagatgg caaacgcgaa
    21121 cgcggtgcca gtggcggcgg cggtcgtttt ggttatgagt tcttccttgc cgatcttgac
    21181 ggcgaagtcc gtgcggatgc atgggcaaaa gaagcagtgc gtatggcgct ggtcaatctt
    21241 tctgccgttg ctgcaccagc gggcaccatg ccggtagtac ttggcgcagg ttggccgggc
    21301 gtgctgttgc atgaagcggt aggtcacggt ctggaaggcg acttcaaccg ccgtggcact
    21361 tcagtattta gtggacaggt cggggagctg gtggcttcag aactgtgtac cgtggttgat
    21421 gacggcacga tggtcgatcg ccgtggttcg gtggcgattg atgacgaagg tacgccaggc
    21481 cagtacaacg tgctgattga gaacggcatt ctgaaaggct acatgcagga taaactcaac
    21541 gcgcgtttga tggggatgac gccgactggc aacggtcgcc gtgaatccta cgcccatctg
    21601 cccatgccgc gtatgaccaa cacctatatg ctgccgggta aatcgacccc gcaagaaatt
    21661 attgaatccg ttgagtacgg tatctatgca ccgaactttg gtggcggtca ggtggatatc
    21721 acctccggca aattcgtttt ctccacttca gaagcatatc tgattgaaaa cggtaaagta
    21781 acgaagccgg tgaaaggcgc aacgttgatt ggttccggta tcgaaaccat gcagcagatt
    21841 tcgatggttg gcaacgacct gaaactggat aacggcgtgg gtgtctgcgg taaagaaggg
    21901 caaagtttgc cggttggcgt gggccagcca acgttgaaag tcgataacct gactgttggc
    21961 ggtactgcgt aataatttca ttattttcag aaggataatt aatctttcta cgtgcacgaa
    22021 cggtcccctc gctcctctgg ggttagggtg aggggaaccc gttggcacag gtttgtagaa
    22081 cgtaacagta caatatgaat tacttctctt tcccgcgccc gtgcatctcc tgaaacaatt
    22141 taccgacctc aacaaaataa tccgtcagcg agttgatcac gacctgtacc ttcagcggca
    22201 gcttatcttt ttcggtatat aacgcataaa ccgggcgtgg atctgactga taacgcggca
    22261 gcaggatctc cagctcccca cgattgatct cgttgatcac ccacatcagc ggcacgtagg
    22321 cgatcccggc acccgccgtt agccagcgca ccagcgtcat cggatcatta gtcacaaatc
    22381 tcccttgcgg gatcaggcga gtcgagatcc cttccggtgc aatcagttca aattcattgt
    22441 cgggccgcac gctgtattca agccatgaat ggctacttaa atcggcgggt ttttccggta
    22501 tgccgtattg tgtgagatag cttttcgcgg cgcacaccac cattggcatc gcgccaagac
    22561 ggcgggaaaa caggctggaa tcctgcaacg cgccgacgcg gatcaccaca tccagaccgt
    22621 cggcaatcag gtcgggggct ggaattccgg taaccagatt ggcgctcaaa cctgggtatt
    22681 ctttcagcat tttggctgtc agcccggcga gaacattttg tgccatagtt gaagaacagc
    22741 caatgcgtag cgtcccgatg ggggtgttat tgaaggcata cagttgctca tgaacatcct
    22801 gcacttcatg aagcatacga cggcagccct ggtagtaaat tctaccggct tcggtcaggc
    22861 caatgctgcg tgtgctacgg tttaacagct ttacctgcaa ctcatcttcc agttttgaca
    22921 ccgtctgact gatggacgaa acgctcatct gtagctgtct ggcggcggcg gtaaaagagc
    22981 caaattcaac tactttggca aacaccgaca tgcgttttag tcgttccatt attcactctg
    23041 acttaaaagt gatttagatc acataatata gataacagca taacagttac gctaatatat
    23101 taaatatcaa tctacagcaa tgttgctctc gcccggcttt ccatgccctt ctctgcggcg
    23161 acagatgctg aaaataataa cgcctgctct ctctatacaa ccaaggtcaa catgagtctg
    23221 tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga tattttttga attgctttta
    23281 tcactggcga ttttctggct ggtgcgccgg gtacttgtgc caacaggtat ctacgacttt
    23341 gtctggcatc cggcgttgtt caacaccgcg ctctattgct gcttgtttta tttgatatcg
    23401 cgactgttcg tttgaggttg aagtgaaaac actaataaga aaattctccc gtacggccat
    23461 cacggtcgta ttagtcattc tggccttcat cgcaattttt aatgcctggg tctattacac
    23521 cgaatccccc tggacgcgtg acgcgcgctt tagcgctgac gtcgttgcga tcgcgccgga
    23581 cgtttctgga ctcattaccc aggtgaatgt tcatgataac cagctggtga aaaaaggaca
    23641 gatactgttc accatcgacc agccgcgcta tcaaaaggcg cttgaggaag cgcaagccga
    23701 tgttgcttat tatcaggtac tggcacagga gaaacgccag gaggccggac gtcgtaaccg
    23761 tctcggtgtg caggcgatgt ctcgcgaaga gatcgaccag gctaacaacg tactacaaac
    23821 ggttctgcat cagttagcga aagcgcaggc gacccgcgat ctggcaaaac tggatcttga
    23881 acgcacggtg atccgagcgc cagcagatgg ctgggtgacc aacctcaacg tctataccgg
    23941 tgagtttatt actcgaggat caacggcggt tgcgctggtg aaacagaact ccttctatgt
    24001 actggcctat atggaagaaa ctaagctgga aggggtgcgt ccggggtatc gtgcagagat
    24061 cacgccgctt ggcagtaaca aagtgctgaa agggactgtt gatagtgttg ccgctggggt
    24121 caccaacgcc agcagcacgc gtgacgacaa agggatggcg accatcgact ctaaccttga
    24181 atgggtgcgt ttggcgcaac gtgttccggt tcgtattcgt ctcgacaacc agcaagagaa
    24241 catctggcct gcgggcacca ctgctacagt ggtggtcact ggcaaacaag atcgcgacga
    24301 aagccaggat tcgttcttcc gtaaaatggc ccatcgcctg cgtgagtttg gttaatcacg
    24361 atgggtattt tctccattgc taaccaacat attcgctttg cggtaaaact ggcgaccgcc
    24421 attgtactgg cgctgtttgt tggctttcac ttccagctgg aaacgccacg ctgggcggta
    24481 ctgacagcgg cgattgttgc cgccggtccg gcctttgctg cgggaggtga accgtattct
    24541 ggcgctattc gctatcgtgg ctttttgcgc atcatcggca catttattgg ctgtattgcc
    24601 ggactggtga tcatcattgc gatgatccgc gcaccattat tgatgattct ggtgtgctgt
    24661 atctgggccg gtttttgtac ctggatatcc tcgctggtac gaatagaaaa ctcgtacgcg
    24721 tgggggctgg ccggttatac cgcgctgatc attgtgatca ccattcagcc ggaaccattg
    24781 cttacgccgc agtttgccgt cgaacgttgt agcgagatcg ttatcggtat tgtgtgtgcg
    24841 attatggcgg atttgctctt ttctccgcga tcgatcaaac aagaagtaga tcgagagctg
    24901 gaaagtttgc tggtcgcgca atatcaatta atgcaactct gtatcaagca tggcgatggt
    24961 gaagttgtcg ataaagcctg gggcgacctg gtgcgacgca ccacggcact acaaggcatg
    25021 cgcagcaacc tgaatatgga atcttcccgc tgggcgcggg ccaatcgacg tttaaaagcg
    25081 atcaatacgc tatcgctgac gctgattacc caatcctgcg aaacttatct tattcagaat
    25141 acgcgcccgg aattgatcac tgatactttc cgcgaatttt ttgacacgcc ggtagaaacc
    25201 gcgcaggacg tccacaagca gctcaaacgc ctgcggagag ttatcgcctg gaccggggaa
    25261 cgggaaacgc ctgtcaccat ttatagctgg gtcgcggcgg caacgcgtta tcagcttctc
    25321 aagcgcggcg ttatcagtaa cacaaaaatc aacgccaccg aagaagagat cctgcaaggc
    25381 gaaccggaag taaaagtaga gtcagccgaa cgtcatcatg caatggttaa cttctggcga
    25441 accacacttt cctgcattct gggcacgctt ttctggctgt ggacgggctg gacttccggc
    25501 agtggtgcaa tggtgatgat tgcggtcgtg acgtcactgg caatgcgttt gccgaatcca
    25561 cgcatggtgg cgatcgactt tatctatggg acgctggccg cgctgccgtt agggctgctc
    25621 tactttttgg tgattatccc taatacccaa cagagcatgt tgctgctgtg tattagcctg
    25681 gcagtgctgg gattcttcct cggtatagaa gtacagaaac ggcgactggg ctcgatgggg
    25741 gcactggcca gcaccataaa tattatcgtg ctggataacc cgatgacttt ccatttcagt
    25801 cagtttctcg acagcgcatt agggcaaatc gtcggctgtg tgctcgcgtt caccgttatt
    25861 ttgctggtgc gggataaatc gcgcgacagg actggacgtg tactgcttaa tcagtttgtt
    25921 tctgccgctg tttccgcgat gactaccaat gtggcacgtc gtaaagagaa ccacctcccg
    25981 gcactttatc agcagctgtt tttgctgatg aataagttcc caagggattt gccgaaattt
    26041 cgcctggcgc tgacgatgat tatcgcgcac cagcgcctgc gtgatgcgcc gatcccggtt
    26101 aacgaggatt tatcggcgtt tcaccgacaa atgcgccgca cagcagacca tgtgatatct
    26161 gcccgtagcg atgataaacg tcgtcggtac tttggccagt tgctggaaga actggaaatc
    26221 taccaggaaa agctacgcat ctggcaagcg ccaccgcagg tgacggaacc ggttcatcgg
    26281 ctggcgggga tgctccataa gtatcaacat gcgttgaccg atagttaagt caaaaccgac
    26341 gccaaaagcg tcggtttttt catggctata cttagcgatg cacggcagaa ctcgccgcga
    26401 aacgtgacgg tggcaacaga tgaatattta tgcctttgat tttgatgaga ttgagagtca
    26461 ggaggatttt tatcgtgact ttagccaaac ctttggtctg gctaaagata aggtacgcga
    26521 tctcgactca ctatgggatg tgttaatgaa cgatgtcctg ccgctaccac ttgagattga
    26581 atttgttcat ctgggagaga aaacgcgtcg ccgttttggc gcgttaatat tgctgtttga
    26641 tgaggcagag gaagagctgg aagggcattt gcgttttaat gttcgtcatt agcgcaaaaa
    26701 aaagcccccg aaccgggggc aatatcgtcg gacaagacga tgagggttta tttgtacagt
    26761 tcagccgtag cgtgccaggt gtcaccgcta cgagcttcag taatctggta ggccgttgcg
    26821 cctttctctt ccgctttttt gttcagcatt tcacgtatat ccattggcga agacgccaca
    26881 ccacttacgg atacggtccc gattgcttca cgattttgtg cttgtgcagc atcaatggag
    26941 tcggcagcga atgcaccgaa agagagaaca gaaagtacgc ttaatgcagc tacagtggtt
    27001 ttgattttca tgatttttac ctcgacataa tcttttagct gggcctttgt ttcgtgaccc
    27061 atatcacaaa atcaagtata cactaattat tgaactaatt aataccacgc taactgtttc
    27121 agttgatact aagtgttaaa aatgtgaatt ttaataacaa aaaagtataa aaaacacggt
    27181 tatttattat taattatagt attttcaatt agataatgaa ttttgcataa tgtgcttttt
    27241 ttaccgtttg tattcatttt gtgaatgatg tgtcgcggta aaacgcacta ttcgttaaat
    27301 gtatcgcagg agaaagaagg ggaatgagag ggataagcaa cattttcccc gccgccagaa
    27361 gcgacggggc agagattaaa gctcctggtc gaacagctct aaaatcgctt cgtacaggtc
    27421 tttgacggtg aaaccgttag caggggtggt aaagatggtg tcatcgccag cgatggtgcc
    27481 cagaatacct tctgctttgc ccagtgagtc cagcaggcga gcaattaact gcgccgcgcc
    27541 agggctggta tgaatcacga caactgcatc gttgtagtcg atatccagca ccagattctt
    27601 caatggactg gaggtggttg gtacacccag ttcagctggc aggcagtaaa ccatttccat
    27661 tttggcattg cgtgtacgta cagcaccaaa cttggtcaac atccgcgaga ctttagactg
    27721 attaatattg tcaaagcctt gctcctgcaa cgcggcgacg atttcgccct gggagctaaa
    27781 tttctcttct ttaagtaatg ctttaaatgc tttaactagt tcttcttgct tagccgagct
    27841 tcgcataagt cacccgatat ggtggttgat acaacattat tgtgcataca gatgaatttt
    27901 tatgcaaaca gtcagccctg aagaaggctg aaataatgtt atgaaagagc gggattttat
    27961 caaatttcgt tattgagaaa catgcctgcg tcacggcatg caaattctgt ttaaaagtaa
    28021 attaattgtt atcaaattga tgttgttttg gctgaacggt agggtatatt gtcaccacct
    28081 gttggaatgt tgcgctaatg cataagcgac tgttaattac gtaagttagg ttcctgatta
    28141 cggcaattaa atgcataaac gctaaacttg cgtgactaca cattcttgag atgtggtcat
    28201 tgtaaacggc aattttgtgg attaaggtcg cggcagcgga gcaacatatc ttagtttatc
    28261 aatataataa ggagtttagg atgaaagtcg cagtcctcgg cgctgctggc ggtattggcc
    28321 aggcgcttgc actactgtta aaaacccaac tgccttcagg ttcagaactc tctctgtatg
    28381 atatcgctcc agtgactccc ggtgtggccg tcgatctgag ccatatccct actgctgtga
    28441 aaatcaaagg tttttctggt gaagatgcga ctccggcgct ggaaggcgca gatgtcgttc
    28501 ttatctctgc aggcgtagcg cgtaaaccgg gtatggatcg ttccgacctg tttaacgtta
    28561 acgccggcat cgtgaaaaac ctggtacagc aagttgcgaa aacctgcccg aaagcgtgca
    28621 ttggtattat cactaacccg gttaacacca cagttgcaat tgctgctgaa gtgctgaaaa
    28681 aagccggtgt ttatgacaaa aacaaactgt tcggcgttac cacgctggat atcattcgtt
    28741 ccaacacctt tgttgcggaa ctgaaaggca aacagccagg cgaagttgaa gtgccggtta
    28801 ttggcggtca ctctggtgtt accattctgc cgctgctgtc acaggttcct ggcgttagtt
    28861 ttaccgagca ggaagtggct gatctgacca aacgtatcca gaacgcgggt actgaggtgg
    28921 ttgaagcgaa agccggtggc gggtctgcaa ccctgtctat gggccaggca gctgcacgtt
    28981 ttggtctgtc tctggttcgt gcactgcagg gcgaacaagg cgttgtcgaa tgtgcctacg
    29041 ttgaaggcga cggtcagtac gcccgtttct tctctcaacc gctgctgctg ggtaaaaacg
    29101 gcgtggaaga gcgtaaatct atcggtaccc tgagcgcatt tgaacagaac gcgctggaag
    29161 gtatgctgga tacgctcaag aaagatatcg ccctgggcga agagttcgtt aataagtaat
    29221 tgattagcga ataataaaaa accggagcac agactccggt ttttgttttg agcactcgac
    29281 ttaattggtt gccggatatt cctgaatggt gacctgcagc gttaactgct tatcatcacg
    29341 catcactaca accgggatca ccgaaccagg gcgaatttcc gccacctgat ccatcgtctc
    29401 cagagcagag atggccggtt tgttatccac cgaaataatc agatcgttga cctgaatacc
    29461 cgcattcgcc gctgggccgt caggtgacac ttcattaacc acgatccctt gcagttgatc
    29521 tataccaccg ctctgcgcgt gcaatggtgc gatctcgcgt ccgccgatac caatgtagcc
    29581 gcggatcacg cgaccatcgc ggatcagctt atccataatt ttggttgcta actggaaagg
    29641 aatcgcaaag ccgatacctt ccggcgtttc gccatcgtta ctcttatcaa acgacagcgt
    29701 gttaatgccc atcagttctc ccagcgagtt caccagcgcg ccgccagagt taccgtggtt
    29761 aatggaagca tcggtttgta ggaagttttg ccgcccggtc gggttcagac cgattcgacc
    29821 cgtggcacta ataatccctt gggtaatggt ctgcccgagg ttgtacgggt taccgatcgc
    29881 cagtactacg tcgccaatgt gcggtacgcg acgtgcatta attggaatgg taggtaaacc
    29941 gccagtggca ttaattttaa gtaccgccag atcggttaga gagtcagatc ccaccagcaa
    30001 tgcttcaaat acacgtccat cctgtaaggc gacgatgatc tgatcggcgt cgttgatgac
    30061 gtgtttattg gtgatgatat aaccgcgttg atccatgatt acaccggatc ccagggtgcg
    30121 gatctcaagc tggttgtgag agttggtgtt caaaccacgg ttgtaaacgt taaccaccgc
    30181 tggcgcggcg cggcgaaccg ccagattata gctggcaggc gtctcatcgg tactgtcaaa
    30241 ttgcggagtg gaaagcgggt taaggctgcg cagcgaaggc atggcaacca gcagaatagc
    30301 gccgacaatt aatccaatcg caacggaacg taagagcttc acaaacatga tggaggcgtc
    30361 attaaaaaag ggaacggcag cagcatacca cgagttaacc ggacatcaca cgtcagcctg
    30421 atgcccggtt tacgacatta acgcatcagc agatagatgc tttcattgcc gcgtacaatt
    30481 tgcagggcga tgatggccgg ttttgccgcc agcactttac gcatttcagc aatcgagttc
    30541 acccgatcgc ggttgacgcc aatgatcaca tcgtcttttt gcaagccagc ctgagcagct
    30601 gggcttcctt tgacaacttc atctatctta atacctttgc cgccatcttt tagctgacca
    30661 tcgctcaacg ttgcaccttc cagcgctggc gtgatcattt cagcgctggc cgacgaagag
    30721 gtgctggtat caagcgtcac ttctacttcc agtggtttgc cgttacgcag caggccaagc
    30781 ttcactttcg tgcccggctc tgtagtcgcg atacgagagc gcaactcagc aaagctattc
    30841 agcggtttgc cgttgaggct ggtaataata tcgcccgctt tgacgcccgc tttcgctgag
    30901 ccagaacttg gcaacacttc gctgacaaac gcgccacgct gcacgtcaag gttgaaggct
    30961 ttggcgatat cggcactcat ctcggtgcct ttgatgccta acaaaccgcg tttgatttca
    31021 ccaaagtcga taagctgctg cgccagtgtt cgcgccatat tactggggat ggcaaatcca
    31081 atcccgacgc tcccgccgcc aggcgcaagg attgcagtgt tgatgccaat taactcaccg
    31141 ttaaggttta atagtgcacc gccggagtta ccgcggttaa tggaagcatc tgtctggata
    31201 aagttttcca gaccttcaag attcaacccg ctgcggccta atgcggaaac aatgccagag
    31261 gtggcggttt gcccaaggcc aaatgggtta ccgaccgcta cggcaaaatc accgacgcgc
    31321 aatttatcgg agtcggcaat agcgatttgc gttaatttgc tcgggttttg aatttgtaac
    31381 agggcgatat cgctctggtc atcgctacca atcagttttg catcaaactc gcgcccatca
    31441 ttgagctgaa tactgatttt ctgtgcctga ttaatcacat ggttgttggt cagcacatag
    31501 cctttactgg cgttgatgat gacaccggaa cctaaacctt cgaagggttg tgcaggttga
    31561 tccggtaaat catcaccaaa aaactttttg aattcttccg ggattttctg tccctgactg
    31621 gccgttcctt ccacccgtac gctcaccact gccggaagca ctttttccag cattggagcc
    31681 agactgggga gaggggcctg atcggcaacc tggcctggaa tcgacgcgac ggcctgaaat
    31741 gacgccgaga gagttaaccc ggcacttaac gctaatgcac tcaacagctg ggtttgtttt
    31801 ttcattattc ctgctctcgt acctgaatga taagaaaaga gattcaaaac agtttgatgt
    31861 tattgaattt tcagacggct aacaatgaga caccagatta aatgatagtc ggggagggag
    31921 agaagaggag ggcgcaatgg ctgcgcccga aaaataaatt agtcgcgctt cgcgccagta
    31981 cgcagcaggc cggatgcgcc ttcagaatag tcgcgaggca tctgcaccgg tgcctgatcg
    32041 ttgctggctt cagactctgc cagacgatta cggaacgggt ttgcttcagc agacagttcc
    32101 ggcagcaggc tgctggagct ttttgccatg tgctgataca gctggcgata gtcgtgcgcc
    32161 atggtatcca gtaattccgc gctgcgggca aagtggctaa ccagctcttc gcgatactcg
    32221 tccagttcag ctttattctt ttccagttcg tactgcaacg cctgttgctg gcgtagttta
    32281 cgattaccaa aacgcatggc cacagcacca ataatgatgc cgacgactaa cccaattagc
    32341 gcatattccc aggtcatgaa catctcccgt tgtcttttgt ttccgtaggg tgttggcttc
    32401 aggctccatg cctgcggctg attatgccac tataaccgtt aattccacag aagtggaatc
    32461 ccgactgcat atcgcgtagt gtagaacggc ctttttttcg tcaaccgtga acaacggcgc
    32521 accgattatt caaggaataa caataagatc atgcaaagcg ttaccccaac atcgcaatac
    32581 ctgaaggcgc tcaatgaagg cagccatcaa cccgacgacg ttcaaaaaga ggccgtcagc
    32641 cgcctggaaa ttatttatca agaactcatc aatagcaggc caccagcccc caggacgagt
    32701 gggctaatgg cgcgggtcgg taagctgtgg ggtaaacgcg aagacacaaa gcatacgcca
    32761 gtgcgtggct tatatatgtg gggcggtgta ggacgcggga aaacctggct gatggacctt
    32821 ttctatcaaa gcctgccggg agagcggaaa cagcgcctgc actttcaccg ttttatgctg
    32881 cgggtgcacg aagagctaac tgccttacag gggcagaccg atccgctgga aattattgcc
    32941 gatcgcttta aagccgaaac tgacgtgctc tgttttgacg aattttttgt ttctgatatt
    33001 accgacgcca tgctacttgg cggtctgatg aaagccctgt tcacccgcgg tattaccctg
    33061 gtagcgacgt caaatattcc gccggacgaa ctttatcgaa atggcctgca acgtgcgcgt
    33121 tttctgcctg caatcgatgc cattaaacag cattgtgatg taatgaacgt ggacgctggt
    33181 gttgattatc gactgcgtac actcactcag gcgcatctgt ggctttcgcc actcaacgat
    33241 gaaacccggg cgcagatgga taaactatgg ttggcgctgg cgggggtgaa acgagaaaat
    33301 tcaccgacgt tagaaatcaa ccatcggcca ttggcgacaa tgggcgtcga gaaccagacg
    33361 ctggcggttt cttttactac gctgtgcgtc gacgcccgca gtcagcatga ctatattgcg
    33421 ctctcacgcc tgtttcacac agtaatgttg tttgatgtgc cagtcatgac gcggttgatg
    33481 gagagcgaag cgcggcgctt tattgcgctg gtggatgagt tttacgagcg ccatgtcaaa
    33541 ttagtggtga gtgcagaagt gccgctgtat gaaatttatc agggtgagcg gctgaagttt
    33601 gagttccagc gttgcctgtc acgtctgcaa gagatgcaaa gcgaagagta tctgaagcgc
    33661 gagcatttgg cgggttaaaa cctgccacaa atcacaaaaa ggggtcgatc tttgaccccg
    33721 acttctctat aatcctgcga ccccacgtta caagaaagtt tttttcccaa aactttttgt
    33781 gtgctggcat aggctattcg aaggggtagg tttgccggac tttgtcgtgt gaacctcaac
    33841 aattgaagac gtttgggtgt tcaccaacgt gtaactattt attgggtaag cttttaatga
    33901 aaacttttac agctaaacca gaaaccgtaa aacgcgactg gtatgttgtt gacgcgaccg
    33961 gtaaaactct gggccgtctg gctactgaac tggctcgtcg cctgcgcggt aagcacaaag
    34021 cggaatatac tccgcacgta gataccggtg attacatcat cgttctgaac gctgacaaag
    34081 ttgctgtaac cggcaacaag cgtactgaca aagtgtacta tcaccacacc ggccacatcg
    34141 gtggtatcaa acaagcgacc tttgaagaga tgattgctcg ccgtcctgag cgtgtgattg
    34201 aaatcgcggt taaaggcatg ttgccaaaag gcccgctggg tcgtgctatg ttccgtaaac
    34261 tgaaagttta cgcgggtaac gagcacaacc acgcggcaca gcaaccgcaa gttcttgaca
    34321 tctaatcggg attataggca atggctgaaa atcaatacta cggcactggt cgccgcaaaa
    34381 gttccgcagc tcgcgttttc atcaaaccgg gcaacggtaa aatcgtaatc aaccaacgtt
    34441 ctctggaaca gtacttcggt cgtgaaactg cccgcatggt agttcgtcag ccgctggaac
    34501 tggtcgacat ggttgagaaa ctggacctgt acatcaccgt taaaggtggt ggtatctctg
    34561 gtcaggctgg tgcgatccgt cacggtatca cccgcgctct gatggaatac gacgagtccc
    34621 tgcgttctga actgcgtaaa gctggcttcg ttactcgtga cgctcgtcag gttgaacgta
    34681 agaaagtcgg tctgcgtaaa gcacgtcgtc gtccgcagtt ctccaaacgt taattggctt
    34741 ctgctccggc agaaaacaat ttgcgaaaaa acccgcttcg gcgggttttt ttatggctaa
    34801 attctgaatc agcgtaaaaa atgggaagtt gctttttgct gccgtctgaa agacaggtaa
    34861 aacaaaaacc acatcgccaa taagggtcta agtcaactat ttcagactaa agcgcatctc
    34921 tttttcccca tttccggcat ccactcacca caaaggtcgc aaaatctggt aaactatcat
    34981 ccaattttct gcccaaatgt cgggtattgc tcattttttg tttgattttc gaacaaagag
    35041 agtcgttcct tattgggtaa cacaacttct gactggccac ctggccacct ggtggctggt
    35101 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35161 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35221 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35281 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35341 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35401 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35461 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35521 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35581 tcctgagcga tgagttcagt ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35641 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35701 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35761 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35821 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35881 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35941 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    36001 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36061 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36121 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36181 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36241 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36301 gttttgtctt tttaatgact taa
//
LOCUS       DADLSB010000023        35831 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.023, whole genome shotgun sequence.
ACCESSION   DADLSB010000023 DADLSB010000000
VERSION     DADLSB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.023"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..510)
                     /locus_tag="JN331_001135"
     CDS             complement(<1..510)
                     /locus_tag="JN331_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7259333.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMMA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFM"
     gene            667..1707
                     /gene="gpr"
                     /locus_tag="JN331_001136"
     CDS             667..1707
                     /gene="gpr"
                     /locus_tag="JN331_001136"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY7259334.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1747..2241)
                     /locus_tag="JN331_001137"
     CDS             complement(1747..2241)
                     /locus_tag="JN331_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY7259335.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2432..3316
                     /gene="yghA"
                     /locus_tag="JN331_001138"
     CDS             2432..3316
                     /gene="yghA"
                     /locus_tag="JN331_001138"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7259336.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3588..4013)
                     /gene="exbD"
                     /locus_tag="JN331_001139"
     CDS             complement(3588..4013)
                     /gene="exbD"
                     /locus_tag="JN331_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY7259337.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(4020..4754)
                     /locus_tag="JN331_001140"
     CDS             complement(4020..4754)
                     /locus_tag="JN331_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY7259338.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            5006..6193
                     /gene="metC"
                     /locus_tag="JN331_001141"
     CDS             5006..6193
                     /gene="metC"
                     /locus_tag="JN331_001141"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY7259339.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6333..6992
                     /locus_tag="JN331_001142"
     CDS             6333..6992
                     /locus_tag="JN331_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7259340.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(7032..7931)
                     /locus_tag="JN331_001143"
     CDS             complement(7032..7931)
                     /locus_tag="JN331_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7259341.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGYKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8125..9288
                     /gene="yqhD"
                     /locus_tag="JN331_001144"
     CDS             8125..9288
                     /gene="yqhD"
                     /locus_tag="JN331_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY7259342.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHFSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9393..10220
                     /gene="dkgA"
                     /locus_tag="JN331_001145"
     CDS             9393..10220
                     /gene="dkgA"
                     /locus_tag="JN331_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY7259343.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            10420..11346
                     /locus_tag="JN331_001146"
     CDS             10420..11346
                     /locus_tag="JN331_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY7259344.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11397..11654
                     /gene="yqhH"
                     /locus_tag="JN331_001147"
     CDS             11397..11654
                     /gene="yqhH"
                     /locus_tag="JN331_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY7259345.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(11697..13916)
                     /locus_tag="JN331_001148"
     CDS             complement(11697..13916)
                     /locus_tag="JN331_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY7259346.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKVSSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(14027..15439)
                     /gene="ftsP"
                     /locus_tag="JN331_001149"
     CDS             complement(14027..15439)
                     /gene="ftsP"
                     /locus_tag="JN331_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY7259347.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(15514..16251)
                     /gene="plsC"
                     /locus_tag="JN331_001150"
                     /gene_synonym="parF"
     CDS             complement(15514..16251)
                     /gene="plsC"
                     /locus_tag="JN331_001150"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY7259348.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(16485..18743)
                     /gene="parC"
                     /locus_tag="JN331_001151"
     CDS             complement(16485..18743)
                     /gene="parC"
                     /locus_tag="JN331_001151"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY7259349.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAAHG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(19289..19771)
                     /locus_tag="JN331_001152"
     CDS             complement(19289..19771)
                     /locus_tag="JN331_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7259350.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(19824..20216)
                     /gene="ygiW"
                     /locus_tag="JN331_001153"
     CDS             complement(19824..20216)
                     /gene="ygiW"
                     /locus_tag="JN331_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY7259351.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            20368..21027
                     /gene="qseB"
                     /locus_tag="JN331_001154"
     CDS             20368..21027
                     /gene="qseB"
                     /locus_tag="JN331_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY7259352.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            21024..22373
                     /gene="qseC"
                     /locus_tag="JN331_001155"
     CDS             21024..22373
                     /gene="qseC"
                     /locus_tag="JN331_001155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY7259353.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(22419..22751)
                     /locus_tag="JN331_001156"
     CDS             complement(22419..22751)
                     /locus_tag="JN331_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY7259354.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            23070..23651
                     /gene="mdaB"
                     /locus_tag="JN331_001157"
     CDS             23070..23651
                     /gene="mdaB"
                     /locus_tag="JN331_001157"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY7259355.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            23682..23996
                     /locus_tag="JN331_001158"
     CDS             23682..23996
                     /locus_tag="JN331_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7259356.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(24044..25936)
                     /gene="parE"
                     /locus_tag="JN331_001159"
     CDS             complement(24044..25936)
                     /gene="parE"
                     /locus_tag="JN331_001159"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY7259357.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPCFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAALVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(25965..26546)
                     /gene="yqiA"
                     /locus_tag="JN331_001160"
     CDS             complement(25965..26546)
                     /gene="yqiA"
                     /locus_tag="JN331_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY7259358.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQVVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(26546..27373)
                     /gene="cpdA"
                     /locus_tag="JN331_001161"
     CDS             complement(26546..27373)
                     /gene="cpdA"
                     /locus_tag="JN331_001161"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY7259359.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(27398..27820)
                     /locus_tag="JN331_001162"
     CDS             complement(27398..27820)
                     /locus_tag="JN331_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY7259360.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(27821..28450)
                     /gene="nudF"
                     /locus_tag="JN331_001163"
     CDS             complement(27821..28450)
                     /gene="nudF"
                     /locus_tag="JN331_001163"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY7259361.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            28655..30136
                     /gene="tolC"
                     /locus_tag="JN331_001164"
     CDS             28655..30136
                     /gene="tolC"
                     /locus_tag="JN331_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY7259362.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQMKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            30284..30955
                     /locus_tag="JN331_001165"
     CDS             30284..30955
                     /locus_tag="JN331_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY7259363.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            30961..32121
                     /locus_tag="JN331_001166"
     CDS             30961..32121
                     /locus_tag="JN331_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7259364.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTNLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(32159..32974)
                     /gene="ygiD"
                     /locus_tag="JN331_001167"
     CDS             complement(32159..32974)
                     /gene="ygiD"
                     /locus_tag="JN331_001167"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY7259365.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADITMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            33090..33863
                     /gene="zupT"
                     /locus_tag="JN331_001168"
     CDS             33090..33863
                     /gene="zupT"
                     /locus_tag="JN331_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY7259366.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(33921..34091)
                     /locus_tag="JN331_001169"
     CDS             complement(33921..34091)
                     /locus_tag="JN331_001169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259367.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(34352..35005)
                     /gene="ribB"
                     /locus_tag="JN331_001170"
     CDS             complement(34352..35005)
                     /gene="ribB"
                     /locus_tag="JN331_001170"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY7259368.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(35109..35257)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            35319..35669
                     /gene="ubiK"
                     /locus_tag="JN331_001171"
     CDS             35319..35669
                     /gene="ubiK"
                     /locus_tag="JN331_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY7259369.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
BASE COUNT         9011 a         9450 c         8903 g         8467 t
ORIGIN      
        1 cataaactca attgcggcaa agaaatcatt catcagtttg gttggatcaa cctgctgttg
       61 cagctcacga cctttatcat catttcccgg ataacctcca acggaactta atccgtcagg
      121 tgccagggcg atataccccg ccttcgccac tcgccgtgcc acatcttcga tatacggatt
      181 cagtccacga ttctcatgca ccaccaccac ggctggcgtt ttgccgctca tttttgcggg
      241 tttcaccaga taaccccgta cctcgccgtg accatttggc gaaggatacg tgatgtactc
      301 agcaacaatc tccgggtcgg taaactctac ctgagtcgcc agcgcataat ttggcttgag
      361 caaatcaaac aacgctaatg ccatcatccc gcccaccgca tacttcgccg caaggttgag
      421 gaactcacgt ttcgaaattt tcccgtgagc gtaatagtcg tagtaatcca acaactcttg
      481 tgggaaatct ttggcggtta aacgcggcat cgttacattc ctcaactggt gtttttttaa
      541 gcaaagcata agcgcgtgtt tttgcccagt ttttcgtcac tctgtgagcc agactacggg
      601 atacgcgctg gcgaatcgct aaactagaaa cattgtttcg aaattgaacg gtggaaagga
      661 gaggtcatgg tctggttagc gaatcccgaa cgttacgggc agatgcagta ccgctattgc
      721 ggaaaaagtg gtttacgcct gcccgcgtta tcgctcggtt tatggcacaa tttcggtcac
      781 gttaacgcgc tggaatcaca gcgtgcaatc ctgcgtaaag cgtttgattt aggcattacg
      841 cactttgatt tagccaacaa ttacgggccg cctccaggaa gcgcagaaga gaactttggt
      901 cgcctgctgc gcgaggattt tgccgcttat cgcgatgaac tgattatctc taccaaggct
      961 ggctacgata tgtggcccgg cccgtacggc tctggcggtt cacgtaaata cctgctcgcc
     1021 agcctcgacc aaagcctgaa gcgtatgggg cttgagtatg tcgatatctt ttactctcat
     1081 cgcgtcgatg aaaatacgcc gatggaagaa accgcctctg cgctggctca tgcggtacaa
     1141 agcggtaagg cgctgtatgt cgggatctcc tcttactcgc cagagcggac gcaaaaaatg
     1201 gtcgagttgc tgcacgagtg gaaaattccg cttttaattc atcaaccttc gtacaattta
     1261 ctgaaccgct gggtggataa aagcggcctg ctggataccc tgcaaaataa cggcgtgggc
     1321 tgtattgcct ttactcctct ggctcaggga ttgctgaccg gaaaatatct caacggcatt
     1381 ccgcaagatt cacggatgca tcgtgaaggg aataaagttc gtggtctgac gccgaaaatg
     1441 cttaccgaag ccaacctcaa cagcctgcgc ttattgaatg aaatggcaca gcagcgtgga
     1501 caatcaatgg cgcaaatggc gttaagctgg ttgctgaaag atgatcgcgt gacgtcggta
     1561 ttgattggtg ccagccgcgc ggagcaacta gaggagaacg tgcaggcgct gaataatctg
     1621 acatttagca ccgaggagct ggcgcagatt gatcagcata tcgccgatgg cgagctgaat
     1681 ctgtggcagg cgtcgtccga taaatgacct gttaataacg ggcgcgcgaa ccgcgcccga
     1741 atcagatcag tgattatgac gagtcagtcg gtcaagatag cccatcacaa atgcagaaag
     1801 cacaaacgtc agatggataa tgacgtacca catcagtttg ttatccggga catttttcgc
     1861 atccataaag acgcgcagta agtgaatgga agaaattgcc acaatcgacg ctgctacttt
     1921 gtttttcagc gacgttgcgt ccattttccc cagccagttc agcttctctt tgttctcgga
     1981 gatatccagc tgtgagacga aattctcata accggaaaac atcaccatca ccagcaaacc
     2041 gccaaccagc gtcatatcca ccagcgacag caacacgagg atcaaatctg attccgccat
     2101 cgagaagata ttcggcagta cgtgaataat ctcctggaag aacttcagcg ccagggcaac
     2161 taacgccagc gaaaggccaa agtacacggg ggcaagcagc cagcgagaag catacattgc
     2221 attttcaaga aaacgttcca tacattcctg tcgtgtagtt aaaacgggca acagtatatc
     2281 gcaattgagt aaacagcacg caacagcaaa tcaacatata cgttaaacct ttgtcgcata
     2341 attctgaggt ttagggatct tcctggttcc cttatcaacg catcgagagc gtctaccctt
     2401 aaaggacgtt gaacctgaag ggagaaaaac gatgtctcat ttaaaagacc cgaccacgca
     2461 gtattacact ggtgaatatc ccaaacagaa acaaccgacg ccaggcatcc aggcgaagat
     2521 gacaccgata ccggattgcg gcgagaaaac ctatgttggt agcggtcgcc tgaaagatcg
     2581 taaagcactg gtgacagggg gcgattccgg aataggtcgc gctgccgcca tcgcttacgc
     2641 gcgtgaaggg gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag acgctcagga
     2701 tgtgaaaaag atcattgaag aatgcggacg caaagccgtt ctgctgccag gcgatttaag
     2761 cgatgagaaa ttcgcccgtt cgctggttca cgaagcgcac aaggcgttag gcgggctgga
     2821 tattatggcg ctggtcgccg ggaaacaggt tgccattccg gatattgcag acctcaccag
     2881 cgaacagttt caaaagacct ttgccattaa cgttttcgcg ctgttctggc taacccagga
     2941 agcgatcccc ctgctaccga aaggtgcaag tatcatcacc acttcgtcaa tccaggcata
     3001 ccagccaagt ccgcatttac tggactatgc ggctacgaag gcggcgattc tgaactacag
     3061 ccgtggcctg gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg tcgcgccagg
     3121 ccctatctgg acggcactgc aaatttccgg cggacaaacg caggataaga tcccgcagtt
     3181 tggtcagaaa acgcctatga agcgtgcggg gcaaccggcg gaactggccc ctgtatatgt
     3241 ttatctggca agtcaggagt cgagctacgt caccgcagaa gtgcacggcg tgtgcggcgg
     3301 cgagcattta ggttaaaaaa atgcccggtt gtgaaaagca accgggcatc attgtgaatt
     3361 cactcttacc tgatgtggcg aaaacgcctt atccgcctac aaacgcatga agattcaaca
     3421 tgttgcaata gattttgtag gcctgataag cgtagcgcat caggcattga gcacctaatg
     3481 ccggatgcgt cgtgaacgcc ttatccggcc tacaaacgca cgaagattca acatgttgca
     3541 atagattttg taggcctgat gagcgtagcg catcaggcaa ttctacttta cttcgctttg
     3601 gcggtttctt cgccgaccag acctatcttc aggtaacccg cctgatgcag cgtatccatt
     3661 accttcatca acgtctcgta atcgacggtt ttgtccgctc ggaagaagat ggtggtgtct
     3721 ttcttgcctt cggttaacgc attcaacgcc gtaatcattg tttcatcggt gaccggatcg
     3781 ttaccgataa acatcgagtt gtctgccttc accgacagat aaaccggttt ttccgggcgc
     3841 ggctgcggcg tgctggtaga agcaggcaag ttcaccttca catctaccgt cgctaacggt
     3901 gccgccacca taaagataat cagcagaacc aacatcacgt cgataaacgg cgtcacgttg
     3961 atatcatgca tttcgccgtt atcgtcgagg ttttcgttaa gatgcattgc catcggacat
     4021 tatcctgcgc gtaatttttg tgcgacacga accggatgcg cagcggcgct ggcttccaga
     4081 tccaggtcac ggctttgcag caacaatacc tgcgctgcaa catcacccag catcgcttta
     4141 aagccgccaa tctggcgtgc aaatacgtta tagataacga ccgcaggaat cgctgccacg
     4201 aggccgattg ccgttgctaa cagagcttct gcgatacccg gcgcaacgac tgccaggtta
     4261 gtggtctgcg tttgcgcgat accaataaag ctgttcatga tgccccagac cgtaccaaac
     4321 agaccaacga acggagaaat cgcgccgatg gttgccagat agccgttacc gcggcccatt
     4381 tgacgaccca ctgcggcgac ccgacgctcc agacggaagc tggtacgttc tttaatacct
     4441 tcgttatcgt cgctgccttc tgacagttcc agctcattct gcgcttcatt gagcaaatgc
     4501 aggcttaagc ttttgctacc aaaatcagcg gcgatatcgt tggcctggtt taaggaacgt
     4561 gcttcagcca gcagttgctg ctcgcgctta aggcgacgct tctgattgaa gaactctacg
     4621 ctcttactga agaagattgc ccaggtgact acggaggcca aaataagccc aatcatcacg
     4681 cacttaacga caatatcggc gtgctgatac ataccccaga cggaaaggtc cgtctgcatt
     4741 aaattattac ccactgtgta tctccaggac gcaagtcaca aaatctgcgc ataataatat
     4801 caaaacgacg tcgaattgat agtcgttctc attactattt gcatactgcc gtacctttgc
     4861 tttcttttcc ttgcgtttac gcagtaaaaa agtcaccagc acgccatttg cgaaaatttt
     4921 ctgctttatg ccaattcttc aggatgcgcc cgcgaatatt catgctagtt tagacatcca
     4981 gacgtataaa aacaggaatc ccgacatggc ggacaaaaag cttgatactc aactggtgaa
     5041 tgcaggacgc agcaaaaaat acactcttgg cgcggtaaat agcgtgattc agcgcgcttc
     5101 ttcgctggta tttgagagta tggaagccaa aaagcacgcg acgcgcaatc gcgccaatgg
     5161 cgagttgttc tatggacggc gcggaacgtt aacccatttt tccttacaac aagcgatgtg
     5221 tgaactggaa ggtggcgcag gctgcgcgct atttccctgc ggggcggcag cggttgctaa
     5281 ttcgattctt gcttttgtcg aacagggcga tcatgtgttg atgaccaaca ccgcctatga
     5341 accgagtcag gatttctgta gcaaaatcct cagcaaactg ggcgtaacga catcgtggtt
     5401 tgatccgctg attggtgccg atatcgttaa gcatctgcag ccaaacacta aaatcgtgtt
     5461 tctggaatcg ccgggttcca tcactatgga agtccacgac gttccggcga ttgttgccgc
     5521 cgtgcgcagt gtggtgccgg atgccatcat tatgatcgac aacacctggg cagccggtgt
     5581 gctgtttaag gcgctggatt ttggcatcga tgtttctatt caggccgcca ccaaatatct
     5641 ggttgggcat tcagatgcga tgattggcac tgccgtgtgc aatgcccgtt gctgggagca
     5701 gctacgggaa aatgcctatc tgatgggcca gatggtcgat gccgataccg cctatataac
     5761 cagccgtggc ctgcgcacat taggtgtgcg tttgcgtcaa catcatgaaa gcagtctgaa
     5821 agtggccgaa tggctggcag aacatccgca agttgcgcga gttaaccacc ctgccctgcc
     5881 tggcagtaaa ggacacgaat tctggaaacg agactttaca ggcagcagcg ggctattttc
     5941 ctttgtgctt aagaaaaaac tcaatgatga agagctggcg aactatctgg ataacttcag
     6001 tttattcagc atggcctact cgtggggcgg gtatgaatcg ttgatcctgg caaatcaacc
     6061 agaacatatc gccgccattc gcccacaagg cgagatcgat tttagcggga ccttgattcg
     6121 cctgcatatt ggtctggaag atgtcgacga tctgattgcc gatctggacg ccggttttgc
     6181 acggattgta taacattgcc acttttggac aattttgcag acattttatt gtgaaaagtc
     6241 ttaaattgtt gcgtccggga tcaaggcgtc ccggacgatt caggagtaca ataggcagat
     6301 aaaggcttaa acgctgttcc acaggaaagt ccatggctgt tattcaagat atcatcgctg
     6361 cgctctggca acacgacttt gccgcgctgg cggatcctca tattgttagc gttgtttact
     6421 ttgtcatgtt tgccacgctg tttttagaaa acggcctgct gcccgcctca tttttgccag
     6481 gcgacagctt gttgatactg gcaggcgcat tgattgccca aggggttatg gattttctgc
     6541 ctacgattgc gattctgacc gccgcagcaa gtctgggctg ctggctaagt tatattcagg
     6601 ggcgctggtt agggaatacc aaaacggtga aaggctggct ggcacagctt cctgctaaat
     6661 atcaccagcg cgccacctgc atgtttgacc gccacggtct gctggcgctg ctggctggac
     6721 gttttcttgc atttgtccgt acgctgctgc caaccatggc gggaatttcc ggtctgccaa
     6781 accgccgctt ccagtttttc aactggttaa gtggattgct gtgggtcagc gtggtaacca
     6841 gttttggcta tgccttaagt atgattccgt tcgttaaacg ccatgaagat caggtaatga
     6901 cgttcctgat gatcctgcca attgccttgt taaccgctgg cttgttaggc acgctgtttg
     6961 tggtgattaa aaaaaaatac tgtaacgcct gacgattttc cccgttcccg gctgctgtac
     7021 cgggaacgta tttaattccc ctgcatcgcc cgcattcttg ccgcatcttc ccccggcgtc
     7081 acaccgaagt aacgtttaaa ctcacggcta aattgcgatg cgctttcata gccgacgcgc
     7141 atcgctgctg cgctggcctt catgccgtca tggatgatca tcatccgcgc cttatgcaga
     7201 cggtaattct tcaaatactg caacggcgag gtgctggtga cagacttaaa attatggtgg
     7261 aacgccgata cgctcatgtt ggcttctgcc gccagttgct cgacgctcag gttttcggtg
     7321 tatttattct caattcgttt cagcacgcgg ctaatcagac tgaagtgagt ctggcgactg
     7381 accagcgcca gtaacgcgcc gccgcaaggt ccggtcagca cgtagtacag aatttcgcgg
     7441 atgatctgtt tgccgagaat acgcgcatcc agtggtcgct ccatcacgtc gagtaaccgc
     7501 tccgccgcgc ataaaatctc ttctgataac gtggcggagt taatcccgct ggctgccatc
     7561 gacggctgga aatgctcatc ttcgccaatg tccatcaaca gttcctgtaa ctgcaaaata
     7621 tcgacattga gacgcaaccc tgccagcggc acctctgacg tggcataggt ttcgcactca
     7681 aacggcaacg gcaccgtcag cagcaggtat tcattggcat cataacgaaa cacgcgttca
     7741 ttgatataac cgattttata cccggaaaag agaattatga tgccaggctc gtacatcacc
     7801 ggtgtacgtg cgaaaggcgt ctcgccatac aacaaacgca catcgggcaa cagttctgac
     7861 aaactatttt ctttattttt cagtttatta actttatccg ccagcaagcg acaaatctct
     7921 tcacgtttca tatcgcgtaa tttcttagga ataatgcggc aatttgattg tgcgcaattt
     7981 tgtagcattt ctccagcact ctggagaaat aggcaagaca ttggcagaaa tgagcattga
     8041 gagccagggc gctggcgatc acaatgaaaa acatcaggca gatcgttctc tgccctcata
     8101 ttggcccagc aaagggagca agtaatgaac aactttaatc tgcacacccc aacccgcatt
     8161 ctgtttggta aaggcgcaat cgctggttta cgcgaacaaa ttcctcacga tgctcgcgta
     8221 ttgattacct acggtggcgg cagcgtgaaa aaaaccggtg ttctcgatca agttctggat
     8281 gccctgaaag gcatggacgt gctggaattt ggcggtattg agccaaaccc ggcttatgaa
     8341 acgctgatga acgccgtgaa actggttcgc gaacagaaag tgactttcct gctggcggtt
     8401 ggcggcggtt ctgtactgga cggcaccaaa tttatcgccg cagcggctaa ctatccggaa
     8461 aatatcgatc cgtggcacat tctgcaaacg ggcggtaaag agattaaaag cgccatcccg
     8521 atgggctgtg tgctgacgct gccagcaacc ggttcagaat ccaacgcagg cgcggtgatc
     8581 tcccgtaaaa ccacaggcga caagcaggcg ttccattctg cacatgttca gccggtattt
     8641 gccgtgctcg atccggttta tacctacacc ctgccgccgc gtcaggtggc taacggcgta
     8701 gtggacgcct ttgtacacac cgtggaacag tatgttacca aaccggttga tgccaaaatt
     8761 caggaccgtt tcgcagaagg cattttgctg acgctaatcg aagatggtcc gaaagccctg
     8821 aaagagccag aaaactacga tgtgcgcgcc aacgtcatgt gggcggcgac tcaggcgctg
     8881 aacggtttga ttggcgctgg cgtaccgcag gactgggcaa cgcatatgct gggccacgaa
     8941 ctgactgcga tgcacggtct ggatcacgcg caaacactgg ctatcgtcct gcctgcactg
     9001 tggaatgaaa aacgcgagac caagcgcgct aagctgctgc aatatgctga acgcgtctgg
     9061 aacatcactg aaggttccga cgatgagcgt attgacgccg cgattgccgc aacccgcaat
     9121 ttctttgagc aattaggcgt gccgacccac ttctccgact acggtctgga cggcagctcc
     9181 atcccggctt tgctgaaaaa actggaagag cacggcatga cccaactggg cgaaaatcat
     9241 gacattacgt tggatgtcag ccgccgtata tacgaagccg cccgctaagc tttttacgcc
     9301 tcaaactttc gttttcgggc atttcgtcca gacttaagtt cacaacacct caccggagcc
     9361 tgctccggtg agttcatata aaggaggaac gtatggctaa tccaaccgtt attaagctac
     9421 aggatggcaa tgtcatgccc cagctgggac tgggcgtctg gcaagcaagt aatgaggaag
     9481 taatcaccgc cattcaaaaa gcgttagaag tgggttatcg ctcgattgat accgccgcgg
     9541 cctacaagaa cgaagaaggt gtcggcaaag ccctgaaaaa tgcctcagtc aacagagaag
     9601 aactgttcat caccactaag ctgtggaacg acgaccacaa gcgcccccgc gaagccctgc
     9661 tcgacagcct gaaaaaactc cagcttgatt atatcgacct ctacttaatg cactggcccg
     9721 ttcccgctat cgaccattat gtcgaagcat ggaaaggcat gatcgaattg caaaaagagg
     9781 gattaatcaa aagcatcggc gtgtgcaact tccagatcca tcacctgcaa cgcctgattg
     9841 atgaaactgg cgtgacgcct gtgataaacc agatcgaact tcatccgctg atgcaacaac
     9901 gccagctaca cgcctggaac gcgacacaca aaatccagac cgaatcctgg agcccattag
     9961 cgcaaggagg gaaaggcgtt ttcgatcaga aagtcattcg cgatctggca gataaatacg
    10021 gcaaaacccc ggcgcagatt gttatccgct ggcatctgga tagcggcctg gtggtgatcc
    10081 cgaaatcggt cacaccttca cgtattgccg aaaactttga tgtctgggat ttccgtctcg
    10141 acaaagacga actcggcaaa attgcaaaac tcgatcaggg caagcgtctc ggtcccgatc
    10201 ctgaccagtt cggcggctaa cgtgcaaatt ctcccggtgg cggtaatgtt ccgctaccgg
    10261 acttttcaga aatcatttat tcccctcgcg tcccgcccgt tgttactctt ccttgttcag
    10321 gaatgccaaa tataaggaca tcatcatgca gagccggaag ctcttaaaag aacaactcat
    10381 ctatatctgg gataaacgca acggagaggt gaaaaacaga tgaaaataat acttctgttt
    10441 ttagcagccc tggcaagttt taccgtacac gcgcagcctc cttcacagac cgtagaacaa
    10501 acagtccggc agatttatca gaactataaa tcagatgcca gtacgcctta tttcggtgaa
    10561 accggagagc gggcgataac ttcagcgcgt attcaacagg cgcttaccct gaacgacaat
    10621 cttacactac cgggcaatat tggctggctg gattatgatc cggtttgtga ttgtcaggat
    10681 tttggcgatc tggtgttaga aagcgtagcg ataacccaaa ctgacgtcga tcatgccgat
    10741 gccgttgtgc gctttcgtat ctttaaagat gataaagaaa agaccacaca gacactgaaa
    10801 atggtggcgg aaaatggtcg ttgggtcatt gatgatattg tcagcaatca tggcagcgtc
    10861 ttacaagcag ttaatagcga gaatgaaaaa acgctggccg ctttagcttc gttgcagaaa
    10921 gaacagccag aatcctttgt tgctgaactc tttgaacata ttgctgatta tagctggccg
    10981 tggacgtggg tggtttccga ctcttaccgc caggcggtta atgccttcta taaaaccacc
    11041 ttcaagacgg ccaataatcc cgatgaagat atgcaaatag aacggcaatt tatttacgac
    11101 aatccgatct gttttggcga agagtcgcta ttttcacgcg ttgatgaaat tcgagtcctg
    11161 gagaaaaccg ccgattccgc ccgcattcat gttcgtttta cgctgaccaa tggcaacaat
    11221 gaagagcaag agctggtttt acagcggcgc gaaggtaagt gggaaatcgc tgattttatc
    11281 cgcccgaaca gcggcagcct acttaagcag attgaggcaa aaaccgccgc cagattaaag
    11341 caatgagctg aattaaataa caattagccg gaacaataaa taaaagggaa cactatatga
    11401 aaacgatttt caccgtggga gctgttgttc tggcaacctg cttgctcagt ggctgcgtca
    11461 atgagcaaaa ggtcaatcaa ctggcgagca atgtacaaac attaaatgcc aaaatcgccc
    11521 ggcttgagca ggatatgaaa gcactacgcc cacaaatcta tgctgccaaa tccgaagcta
    11581 acagagccaa tacgcgtctt gatgcacagg actattttga ttgcctgcgc tgcttgcgta
    11641 tgtacgcaga atgataaaaa aatccccggc agcatgtcag ttgccgggga ttttttttaa
    11701 cgtccaaccg ccgctttagg gcgtttcttc gcaccagcat tcaccggacg agattgcgta
    11761 gacgacactt tttttgccgt agcaggcgtc tgacgctgag tcgccatcgg cgtatgttta
    11821 gtcaacgccg gacgggtatt gcggttctgg cgacgagctt cacgcatctc ttcaatggtt
    11881 ggcgcaggca ctaagcaatc gcgacggctg ccaatcagat gctttttgcc catcgcttcc
    11941 agcgcctggc ggattaacgg ccagtttgcc ggatcgtggt aacgcaacaa cgctttatgc
    12001 aaacgacgct gtttgtcgcc cttcggtacg aagacgtctt cgctcttata accaatcttc
    12061 gccagcgggt ttttcccggt gtaatacatg gtggttgagt tcgccagcgg cgatgggtag
    12121 aagttctgta cctggtcgag acggaaacga tgctttttca gccacagcgc caaattcacc
    12181 atatcttcat cacgcgtacc ggggtgcgca gagatgaaat acgggatcag atactgctct
    12241 ttacctgcct gtttcgagta ggtatcgaac agctctttaa agcggtcata gctgcccatg
    12301 cccggcttca tcatcttcga caacggccct tcttcggtat gttccggagc aatcttcaga
    12361 taaccgccga cgtgatgggt cgccaactct ttgatatagc gcggatcttc cacggcgatg
    12421 tcataacgta cgcctgaggc gatgaggatt tttttaatgc ctttcagatc acgcgcacgg
    12481 cgatagaggt tgatcgtcgg ttcgtggtta gtgtccatgt gcggacaaat atccggataa
    12541 acgcacgaca aacggcgaca agtttgttca gcgcgtggcg atttgcagcg caacatatac
    12601 atgttggcag ttggcccacc gagatcggaa atcacgcccg taaaacctgg aacggtgtcg
    12661 cggatcgctt cgatctcatt aatgatcgaa tcttccgaac ggctctgaat aatgcgccct
    12721 tcgtgctcgg taatagaaca gaaagaacag ccgccaaagc agccgcgcat aatgttgacc
    12781 gaaaaacgga tcatttcgta agccggaata cgggcattgc cataggccgg atgtggcacg
    12841 cgcttgtacg gcagcgcaaa aacgctgtcc atctcttcgg tagaaagcgg gatagcaggc
    12901 gggttgatcc acacatagcg gtcaccgtgt ttttgcatca atgcgcgggc gcagcctggg
    12961 ttggtttcgt ggtgcagaat gcgcgaagca tgggcgtaca gcactttatc gcccttcact
    13021 ttctcgaaag aaggcagcaa tacgtaggtt ttttcccacg gtttctggcg cggtggctgc
    13081 acgattacag ctttcgcttc ctgctttttc ggcgcaacag gtttgttatc cgcacacggc
    13141 aaatcttcgc catacggatg cgggattggg tcgattttcc ctggggtatc aagacgggtg
    13201 gaatccacac cgctccagcc aggcagcgcc tcttttacga taattgcggt attacgcaca
    13261 tcgcggattt cactaatcgg ctcgcccatc gccagacgat gcgctacttc caccagcgga
    13321 cgttcaccgt taccaaacat cagcatgtcg gctttcgaat ctaccagaac ggaacggcgc
    13381 acggtatcgg accagtaatc ataatgcgcg gtacggcgca ggctagcctc aataccgccg
    13441 aggatcaccg gcacatcttt ccacgcctct ttacaacgct gggtataaac cagcgtggcg
    13501 cgatccggtc gcttacccgc gacattatct ggcgtgtagg catcatcatg acgtaaacgg
    13561 cgatcggcgg tatagcggtt gatcatcgaa tccatgttgc cagcagtaac accaaaaaac
    13621 agattcggtt tacccagacg cataaagtcg tctttgctgc tccagtccgg ctgggcgatg
    13681 atcccgacgc gaaagccctg cgcttccagc atacgaccgc aaatcgccat cccgaagctt
    13741 gggtgatcga catacgcgtc gccagtaacc aaaatgatgt cgcagctatc ccagccaagt
    13801 tgatccatct cttcacgaga catcggcaaa aacggtgccg gtccaaaaca ggcggcccag
    13861 tactgcggcc aggagaacag gtcgcgatcc ggttggatca gggagataga gctcattttg
    13921 cttccagaaa tgataaaaaa ataatcaaag gccggggatt ataagccgga acgaaagaga
    13981 aatcgaaagg tattccatac tcgccctcct cgggcgagta tgaagattac ggtaccggat
    14041 tgaccaacag ttgcccaatc gagccacggt ccgccatttc cagcgtctga ctgttgaagt
    14101 agaacgggaa gtgcgcccag gaaggctgac cgaaataaac aagcagctcc acttgtccgt
    14161 caacccaaac ggtatctttc cagcctctgt cttccgggaa cggcatcgcg ccattcacgt
    14221 tacggatctg gaacattacg ccttcaatat ggaacgcttg cggctcgtcc gcgcgtaccg
    14281 tccagcgttc ccacgttcct tgctgcgcgg tgacatcaat acggttgacg tcccacagct
    14341 gtccattaat acccgggtca tcacccagac tgatatcgcg actgcgaatt ggcgaaccag
    14401 ccatgatttc agttggcagc aagcgcatcg gaagactgtc tgtgaccagc ggcagaaggc
    14461 cggttgggcg cagcgttagc accaaggtag aaaccagaat actggatggc tcaaagaagc
    14521 cgcgaatacg atcaacaatg ctcgccgctt cgccacaggt gatcgacact tcatcgccgt
    14581 tgctcatatc caccagaatc tcgcggcgct cgcccggtgc cagcgaaagt tgcttcaccg
    14641 atacaggagc aggcaggaat ccctgatcgc cagaaatcac atgtaacggg cgaccatcgc
    14701 tcatctgtag ttgatagcga cgagagttcg acgcgttcag cagtcgcaag cgcacccagc
    14761 cacgcgagac ttcaacgtac gggctttgta caccgttaac cagcagcgta tcaccaacaa
    14821 agccgccgct tcccggttcg ttgtattctg gcgtaccaaa gttatccagc cgtttatcct
    14881 ggataatgac cggaaaatca tccacaccat aatggttggg gataggcagc gacttgctga
    14941 cttcatcttc caccagccac attccggcaa ggccgttata gacctgctga gccgtgcggt
    15001 tgggagtatt ggcgtgatac cacagagtag ctgcgttctg gcgaataggc agtacgggtg
    15061 cccagtcagc gtttggcgac atcatccgtg ccggaccgcc catcagcggg cctggtacct
    15121 gtagcccggc caccgtcatt gagacatttt ctgtcaggcg gttgctgtaa ataagcttaa
    15181 catcgtcgcc cttccagacg cggatagtcg gccccaggta acgaccattg attccccaga
    15241 ccgacgcgcg tgtccctggc gtaaatgacc agtgcgcacg ttgtacagtc ataaacagcg
    15301 gttgcccacg gcgagattca agtagcggcg gaacgggtag cggttgctgt tgcccggctg
    15361 cgctggcctt caggggaaca gcgcctgcac aaagtgcaat ccccgatgcc tgaatgaact
    15421 gacgccgact gagtgacata tttgctccat gtaaaactgg ctaaagtaat accgtgcagg
    15481 aaagtgttcc ccgcaaaaat acagattgcc gacttaaact tttccggcgg cttcgcgttc
    15541 tgcgacttct ttatcgagct cggcgatttt ttgttccatt atcgaacgac aatgggcagc
    15601 cagctcacga acctgatctt tgccatactg actgacgtca attggcggca gcatttcgac
    15661 aatcaccaga ccgttgtgca gtcgattaag attaatctta ttcgaagttg tagagacgca
    15721 cacgggaata atcgggacgc ccgccgcaat tgccgcgtga aatgctccag tcttgaacgg
    15781 tagcaggccg cgaccacggc tgcgggttcc ttccgggaac atccagatgg aaatacggcg
    15841 ttttttgaaa tgattcacga cttccgcaat ggtgccgtga gctttagtgc gattgtttct
    15901 gtcgatcaat aagttgccgg ttaaccagta caactgcccg aagaagggga tccacagcaa
    15961 gctcttttta cctaccgtca ccgtcggcgg ttgcacaatg ttcgatgctg tcaccatgtc
    16021 atagttgttc tggtggttag cgatatagat agcattgccg tagctttcag catctgcagg
    16081 tttgcggcac tcaactttca ggccgaacag tggcgcaaga cggccaaaca tatgcccaaa
    16141 ggtggccaca tgtttcgggt tacgcgggct gaaaaggcag taaatggagc cgaatacaca
    16201 gactaagatg ctgtaaatca cggtaataat aagacgaaag atatatagca taacaccctc
    16261 tgaaggtctg agagactggc attgtacgtc acctgaaatc aggttagggc attgttaaaa
    16321 gccgctctct ggaaaagcgt attgttaatc gcaacgcatg aataaacaac ggtttcaagg
    16381 gaatttttat cgtttccggg ggttgaatac gctgccggat gacgacttaa cgtttcatcc
    16441 ggcgttcctt gctagcggga ggaaacagcg ccctccccgg catattactc ttcgctatca
    16501 ccgctgctgg cacggcgagg agagtcgatc tcaacacgat cgatacgctg caaaccgcgc
    16561 atcaacgtac cgcggcgtcc acgttcgcca gtgactttct gtagctcttc cggacgcagt
    16621 ttaattttgc gtttcccaac atgaatggtc agcgtgcttt gcggcggcag aacgtacagt
    16681 tgcgccagac catcctcgcc atgcgcggct tctgccgatg gaatgttgat aatcttgttg
    16741 cctttgccct tcgacagctg cggcagatcg cttaccggga acatcaacat acggcctgcc
    16801 tgagtgattg ccagcagcat atcggaagca tcttcaatca ccaccggcgg cataacatgg
    16861 gcattttccg gtaaggtgat caaagcctta cccgcacggt tacgcgccac cagatcgtta
    16921 aaggtgcaga cgaaaccgta acccgcatcg gaagccatca gcagtttctg atcgtcgctt
    16981 tccatcagca tatggtcaac ggtcgcccca ggcggcaacg ttaatttgcc ggtgagtggt
    17041 tcgccctgac cacgcgccga cggcagcgta atcgggtcga tggcatagct acgaccggtg
    17101 gaatcaacaa acactaccgg ttggttactc ttacctttca ccgccgcttt gaagctatca
    17161 cccgctttat aattcaggcc cggcgcgtcg atatcatggc ctttagcgct gcgtacccag
    17221 cccatctgcg acagcacaat ggtgacaggt tcagacggca gcatgtcgtg ctcgctcatc
    17281 gctttcgctt cttcgcgttc ctgcaacggc gaacgacgat cgtcaccgta ggcttgcgcg
    17341 tctgcctgca gttctttctt cagcaggtta ttcattttac gctcggaagc caaaatgccc
    17401 tgcaactggt cgcgctcttt ttccagctca ctctgctcac cgcgaatctt catctcttcc
    17461 agtttggcaa gatgacgcag tttcagttcg aggatcgctt ccgcctgggt ttccgtaagg
    17521 ccaaaccgcg acatcagcgc cggtttcggt tcatcttcgt tacgaatgat ctcaatcact
    17581 tcgtcgatgt tgagaaacgc caccagcaaa ccttccagga tatgcaggcg cttgaggact
    17641 ttctccagac gatagttcag tcggcggcgc acggtatcgc gacggaacac cagccattcg
    17701 gagaggattt ccagcaggtt tttcaccgcc ggacgaccat ccagaccgat catattgagg
    17761 ttaatgcgat agctcttttc cagatcggtg gtagcgaaga ggtggttcat cacctgatcc
    17821 atatccacgc ggttggaacg cggcacaatc accagacggg tcgggttctc gtggtcagat
    17881 tcatcgcgca gatcgtcaac catcggcagc tttttgttgc gcatttgcgc agcaatttgc
    17941 tccagtacgc gcgcacctga aacctgatgc ggtaatgcgc tgataaccac cgcgccatct
    18001 tctttcttcc acaccgcgcg catacgcact gaaccacgtc cgttctcgta gattttacga
    18061 atctcggcgc gcgaagtgat aatttccgct tcagtcggat aatccggccc ctgcacgata
    18121 tccagcagct gatcgagcgt ggttttcggc tggtcgatta atgcgattgc cgcctgagct
    18181 acttcacgca ggttatgcgg tggaatatcg gtcgccatgc cgacggcaat accggtggtg
    18241 ccgttaagca aaatgtttgg cagacgggca ggtagcattt tcggctcctg caaagtgccg
    18301 tcgaagtttg gcacccagtc agccgtcccc tgccccagct cgctcaatag cagctcggaa
    18361 tatttcgaca accgggattc ggtgtaacgc attgccgcga acgatttcgg atcgtccgga
    18421 gcgccccagt tcccctgacc atcaaccagc ggataacggt aagagaacgg ctgcgccatc
    18481 aggaccatcg cttcataaca ggcgctatcg ccgtgcggat ggtatttacc cagtacgtca
    18541 ccgacggtac gggccgattt tttaaatttg gcgctggcat tcaggcccag ttcagacatc
    18601 gcatacacaa tgcggcgctg aacaggtttc agaccatcac caataaacgg caacgcacgg
    18661 tccatgatca cgtacatgga gtagtttaag taggcgtttt ccgtaaattc atgtagcgca
    18721 aggcgctctg ccatatcgct cattaattct gattcctcaa cttattcgcc agccttcaaa
    18781 ggggcaatat tgccggagat actacattat ctgccgcgtc gagtcacagg agatcagcgt
    18841 gaatagcgcc gccggacgtg ctccgacggc gataaaggca tcaatgtgcc ttgatataca
    18901 actctttgct gtagacttga tcggcataga gggcagcaca aggttgcgcc catgccacac
    18961 cactcggcat gggggtccgc tccgggcgat tcgagaagtt gaggttatgc gagtccaggg
    19021 aagcctaaag catcaaagta gtcgactgta agtatacggt tcagcccagt ttagctgtgt
    19081 ctccaccagt gccccgcgcc tcctgccata agtgcggctt gctcatggat acccccgaga
    19141 cggcttacgg cgcaccggat aataaatttc gcgtatacat ggtattcctt accggagaaa
    19201 ttataagccc aacaacaata cgcctgtgac attgttcgct caaacttatc gcgagtgatt
    19261 tggctcacac tccggtaaag attccccatc acgccaacgg cacataaaca tccgtttcca
    19321 gtcgctgctc tgtcacccct tcggcaagat tggtgtagtg aaacagaatc ggtgctttac
    19381 gcattttctc gccgcttgca ggcagccagt ggcgaataat gccccatatc gtgtgtgaaa
    19441 tatcgtccag ctcgccaacg tggcgggcca cggcataacg tccaccggta agttcaccat
    19501 tgctcacacc ataacgatta tcgggaatcg gttcgctaac gctgccgcag atatcaaagc
    19561 gaaacgcttc cggtgcggtg gtggcagggt cgtcccaggc gacgccaaaa gtctggcttt
    19621 ggttaacagg agaaagcccc gtctctttac gccacatgat aaatttcgcc acactgtagt
    19681 tgagcaactc agggctacag cggtgcggca acatcgccac agggattgat gggaaatcga
    19741 taatgtttac atccagtgtc aggtttgtca ttttgcctcc ctgcgaaatt attcccggga
    19801 acgcgtcccg ggagcggtaa caattacgga tttactttgc ggatctgttt gacgtcaatt
    19861 tcaacagaat tccagtcttt atcgacttca ccctgaatct caaccgtatc tttcggcgtc
    19921 accgtcacac cgttccagcg tttgtgatcg atatcaacat tgatagtacc gctggcatct
    19981 ttaaacacgt agagatcgtc agagatgcgt tcaacgatat tgccgcgcag ggttacccag
    20041 gtgtcgtcac gcagggattt tgcgctttct acagtcgtta cgctgccgtt cggcccctgg
    20101 aatcctccgg cctgactttg cgttgccgat gggccagaaa aaccgccctg ctctgctgcc
    20161 atcaccggtg cactgcacag ggccattact gcgattactg ctgcgaattt tttcatgttt
    20221 attactccct ttaatgtctg tttccgagca tttaacaaga tagtccttaa caacttctta
    20281 agggaaaaaa ataaaattta atgctgtaca gagcgcgtta caacacggtt tactggcagc
    20341 aaatacggtt atcgcaggga tgaaaaaatg cgaattttac tgatagaaga tgacatgctg
    20401 attggcgacg gcatcaaaac tggccttagt aaaatgggtt ttagtgtcga ctggtttaca
    20461 caaggtcgtc agggaaaaga ggcgctttat agcgcacctt atgatgcggt gatcctggat
    20521 ttaaccttac caggcatgga tggtcgcgat attttgcgcg aatggcgaga gaaaggtcag
    20581 cgtgagccgg tactgaacct gaccgcgcgt gatgcgttag cggaacgtgt agaagggctg
    20641 cgtctgggag ctgacgatta tctgtgtaaa ccttttgcgt tgatagaagt cgccgccagg
    20701 ctggaggctc tgatgcgccg aaccaacggc caggccagca acgagctgcg ccacggtaac
    20761 gtcatgctcg accccggcaa acgtatcgcc acgctggctg gcgaaccctt aacactgaaa
    20821 ccaaaagaat ttgccctgct ggaattactg atgcgtaacg ctggtcgggt actgccgcgc
    20881 aaactgattg aagagaaact gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa
    20941 gtgcatgtgc atcatctgcg acgcaaactc ggtagtgatt ttattcgtac cgtgcatggt
    21001 attggctaca cattaggtga gaaatgaaat ttacccaacg tcttagtctg cgcgtcaggc
    21061 tgacgctaat ctttttaatt ctggcctcgg tgacctggct gctttccagc tttgtcgcct
    21121 ggaaacaaac aacggataac gtcgatgaat tgttcgacac ccaactgatg ctgtttgcca
    21181 aacggttaag tacgctcgat ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc
    21241 caaataaatt aaaacacggt catgttgatg acgatgcgct gacctttgcc atctttaccc
    21301 acgacggcag aatggtcctt aatgatggcg ataacggaga agatattccc tatagctatc
    21361 aacgggaagg ttttgctgac gggcaactgg tcggtgaaga cgatccttgg cgttttgtct
    21421 ggatgacctc acctgatggc aaatatcgca tcgttgttgg ccaggaatgg gaataccgtg
    21481 aagacatggc gctggcgatt gttgccgggc aattgatccc gtggctggtc gcactgccga
    21541 ttatgttaat catcatgatg gtactactgg gtcgtgaact cgcgccgctg aacaaactgg
    21601 cgctggcact acgtatgcgt gaccctgact cggaaaaacc actaaacgcg actggcgtac
    21661 ccagcgaagt gcggccactg gttgagtcgc taaatcaact gttcgcccgc acacatgcga
    21721 tgatggttcg agaacgacgc tttacctccg atgcagctca cgaactacgt agcccgttaa
    21781 cagcgctgaa agtgcaaacc gaagttgcgc agctctctga cgatgatccg caggcgcgga
    21841 aaaaagcact gctccaatta cattccggga tcgatcgcgc tactcgtctg gttgatcaac
    21901 tgctcacgct atcgcggctg gactcactgg ataaccttca ggacgtcgcg gagatcccgc
    21961 ttgaagatct cctacaatcg tcggtgatgg atatttacca cacggcgcag caggcgaaaa
    22021 ttgacgtgcg actgacactc aatgcccacg gcatcaaacg caccggacaa ccgctattgc
    22081 taagtttgtt ggtgcgaaat ttgctggata acgccgtgcg ctacagtcca cagggcagcg
    22141 tgatagacgt cacgctgaat gctgataatt tcatcgtgag ggataacggc cccggtgtga
    22201 caccagaggc actggcgcga attggcgaac gcttctatcg cccacccgga caaaccgcta
    22261 ccggcagtgg acttgggcta tcgattgtcc agcgaatcgc caagctgcat gacatgaatg
    22321 ttgagtttgg gaatgcggaa caaggtggat ttgaggcgaa ggtaagctgg taacttctct
    22381 gtctatgaga gccgttaaaa cgactctcat agattttact aatagcaaaa tataaaccgt
    22441 ccccaaaaaa gccaccaacc acaaaccaga caaataacga gcgagaatac atatagccaa
    22501 aactcgaaca tttcccgcat aaagagtttt cttaagataa gaataataag tggcgtaaga
    22561 agaaaaaatg ctgcaatcat tgcttgcaat atacgatctt cgcctgaaac ggaaggtata
    22621 ctgcaaatat tgcctcccga ctcaagcatc cagtcatact tattgaaagc aaaaaaagaa
    22681 tctattaacc aataagtaag cattatatat agcaatgctt caaatattgt tttaattttc
    22741 tgctttaaca tatatcagta cgctcataaa aatccattta taattatcaa catgacacgt
    22801 tgatattttc atcttttact tattcaaagc catattttca atccccaaaa aaaccgaacc
    22861 aaacgtgtaa attattccgg tgcgagtaag aattatgagg aatggctatc agtattgtca
    22921 ttttcagaaa atatttatcc tgcatcggtg agtcagagct agatcagact tttgctaaat
    22981 tcgcaaaaga ctttgcacat tttgctaatt tcaccgtacc gctctgtgac gtactatagt
    23041 cgacaaacgt ctcaccttga ggttaaaaaa tgagcaacat cctgattatc aacggcgcga
    23101 aaaaattcgc ccactccaat ggtcaactga acgacaccct gaccgaagtc gcggatggca
    23161 cactgcgcga cctcgggcat gatgtccgca tcgttcgcgc cgacagcgac tacaatgtca
    23221 aagcggaagt acaaaacttt ctctgggctg atgtggtgat ctggcagatg ccaggctggt
    23281 ggatgggcgc gccgtggaca gtgaaaaaat atattgatga tgtattcacc gaaggtcacg
    23341 ggactctgta tgccagcgat ggtcgtaccc gcaaagatcc gtcgaaaaaa tacggttccg
    23401 gcggcctggt acagggcaaa aaatatatgc tttctctaac ctggaacgca ccaatggaag
    23461 ccttcaccga aaaagatcag ttcttccacg gcgttggcgt tgacggtgtg tatctgccgt
    23521 tccataaagc aaaccaattc ctcggtatgg aaccgctgcc gacatttatc gctaatgacg
    23581 tgataaaaat gcctgatgtt ccccgctata ctgaagaata tcgcaagcat cttgtggaaa
    23641 tttttggtta actagagctc aggctttaga aggagttaac catgcttacc gtaatcgcag
    23701 aaatccgtac tcgtcctggt caacatcacc gtcaggcggt attggatcag tttgctaaaa
    23761 tcgttccaac cgtactgaaa gaagaaggtt gccacggcta tgcgccaatg gtggattgcg
    23821 ctgctggcgt gagtttccag tctatggcac cggattctat cgtgatgatt gagcagtggg
    23881 aaagcatcgc gcaccttgaa gcgcatctgc aaaccccgca catgaaggcg tacagcgaag
    23941 ccgtaaaagg cgacgtgctg gagatgaata tccgtattct gcagccaggg atttaatcct
    24001 gccttgtttg cccggccatc ctgaccgggc aatgttcttt cctttaaacc tcaatctccg
    24061 ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc cgagcgtttc ttcgccagca
    24121 gcatatccat catcgcgtca gtacgctgat cgtcttcatc atcgatagtc aactgcacca
    24181 gacggcgagt gttcggatca agcgtggttt cacgcaattg catcgggttc atttccccca
    24241 gacctttaaa acgctggacg ttcggcttgc ctttcttgcg ttttaattgc tcaagtacgc
    24301 cctctttctc ttcttccgtc agcgcgtaat aaacctcttt cccgagatca atacggtaga
    24361 gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa cgcgcggaag tgttttacga
    24421 acaaagcgca gagcagcgtg gcaatgtgca gaccatcaga gtccgcatcc gcgaggatac
    24481 agattttgcc ataacgaagc tggctcagat cgtcgctgtc aggatcgata ccgatcgcta
    24541 ccgaaatatc atgcacttcc tgcgaggcca gcacttcgtc agaagaaact tcccaggtgt
    24601 taaggatctt acctttcagc ggcatgatcg cctgatattc acgatcgcgc gcctgcttgg
    24661 cagatccgcc tgcggagtca ccttccacga ggaacagttc ggtacggtta aggtcctgcg
    24721 cggtacaatc agccagtttg ccaggcagcg ccgggccgct ggtcagcttt ttacgcacca
    24781 cttttttagc cgcacgcata cgacgctggg cgctggaaat cgccatctct gccagcaact
    24841 ccgccgcctg aacgttctgg ttcagccaca ggatgaaggc atctttcacc acgcccgaaa
    24901 ctaatgccgc acactgacgc gaagagagac gctctttggt ctgcccggca aactgcggat
    24961 cctgcatttt aaccgacaga acataggcgc agcgatccca gatatcttcc gccgacagct
    25021 tcacgccgcg cggcagaatg ttgcggtatt cacagaactc acgcatcgcg tccaacaggc
    25081 cctgacgcag accattaaca tgggtaccgc cctgcatcgt tgggataagg ttgacgtagc
    25141 tttcggtcag cagttcaccg ccttccggca gccacagtag cgcccagtcc acagcttcag
    25201 tatcgccagc gaaattaccg ataaacggtt tttctggcag cgtcggtaaa ccgtttaccg
    25261 cttccgccag gtaatcattc agaccgtcct gatagcacca gcgctgttcg gtgttgttga
    25321 tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac tgctttggcc ttcagcacat
    25381 gcgtcaggcg tgaaacagaa aaacacgggc tgtcaaagaa ggtttcatcc ggccagaagt
    25441 gcacgctggt accggtattg cgtttaccgc aagtgccgac aacctgtaaa tcctgcacct
    25501 tttcgccatt ttcaaaggcg atgttataaa cctgaccatc gcggcgcacg ttaacttcta
    25561 cgcgcttcga caaggcgtta actaccgaaa tccccacgcc atgcaggccg ccagagaact
    25621 ggtaattttt gttagagaat ttaccgcctg catgcagacg gcaaagaatc agttcaaccg
    25681 ccggtacacc ttcttccggg tgaatatcca ccggcatccc gcgcccatcg tcaatcactt
    25741 ctaacgactg gtcagcatgt aagattacat ccacgcgttt tgcgtgaccc gccagtgctt
    25801 catcgacact gttatcaata acttcttgcc ccaaatggtt agggcgagtg gtatcggtat
    25861 acatccccgg acggcggcga accggctcaa gcccggtgag tacctcaatg gcatcagcgt
    25921 tataagtttg cgtcatggtt taagttagca attcgagttg atcgtcagag atggtgcaga
    25981 ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc ccgtgaatgc gtggttgccg
    26041 ccttctatga cagtctggcg gcaggaagcg tagtacgcca ccacctggcg gtaatccagc
    26101 acttcatctc ccgtctgttg cagcagccag atcaaatccg gcgcttccag cgggtcaatc
    26161 tgcatgactt tgagatcgta aatatggcgt gactctagca catattgctg cccggtgtag
    26221 gggttctcgt tctgaccgag atagtccgtc agcaattcaa acgggcgtac cgccgggttt
    26281 accaccactg cgggcagcat aaaacattgt gacaaccagg tggcgtaata tccccccagt
    26341 gacgaaccga caatacccag cgaatcgccg ccatgttcca ggacaatgga ttccagcagt
    26401 tctgccgcgt cggaaggata cggcggcaac tgcggaatga tcatctcaac gtcagggtga
    26461 tgttccgcca gccagttttt taacaagctc gcttttgcag agcgcggcga gctgttgaaa
    26521 ccgtgtaaat aaagaagcgt agacatcagt agccttctga agcggtatca ggttggaaac
    26581 gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc atcagcatgt aactcgagag
    26641 tacgccagcc gggcgcgata gtatccagcg taaagttgga acagtgcggt ttaaactgca
    26701 cacaggtcga cggcgttgcc agcaggcggc gaccgttcca gtcgagatcc agctcctgat
    26761 gaatatgacc gcacagcaag tatttgacgt gcggaaactt cgccagcacg gtatccagtt
    26821 cgcccgcgtt acgcagactg tgttgatcga gccaactaca acccgcaggt agcggatgat
    26881 gatgcagcag cagcaacgta tggcgttctg gcgcatcggc cagtttacgt tccagccact
    26941 caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa cacctggcta tccagcaaca
    27001 ggatttgcca ttgctcacca ataaacaccc gcttcgccgg ggagataccc gcatcctgta
    27061 acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg cagccagacg cagggcgcac
    27121 gaaaacttgc gatgccttca gcgaaatgct gataggccgc agaggattga tcctgcgcta
    27181 aatcacctgt cgcgacaatc aggtcgaatt cgtgctggtg tggacgaatc gcctccagca
    27241 ccgcctggta gctttcccag gtgtttaccc ctaacagggc ttcgtgcttt tgtgcaaaca
    27301 ggtgagtatc ggtaatttgt aaaatccttc ctctggcctc accagccaga ggaagggtta
    27361 acaggctttc caaatggtgt ccttaggttt cacgacgcta gtaaaccgga atcgccatcg
    27421 ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa ctggttaatt tgatgctttt
    27481 cgtcgcgttg atgcaacttt ttattaggat aatcataccg cgctttgaag cgaaaaatct
    27541 gctggcttga acacacttca gccaccatcg cgtcatgata cagacgcacc gtcattgacg
    27601 gaaggctcca gtaactgatc gcgggcgcag tctgttctat tgtcaccagg gtagtgtatc
    27661 gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc cacctgatag cttacagttt
    27721 cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg tgaaaagttc atctcgcaca
    27781 ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat ttatgcccac tcatttttta
    27841 acgcttgatg atgcagctgc agccattgca aagcgatgac cgacgctgcg ttgtcgattt
    27901 tcccctcttc tacccactgg tatgcctgtt cccggcttac cacatgaacg cgaatatctt
    27961 cgttttcatc agccagacca tgaataccgc ttgcggtcgt ggcgtccact tcgcccacca
    28021 taattgacga acgctcactg gtgccccccg ggcttgccag gaaacttaac accggtttgg
    28081 tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg acgggcaaca tcttccacac
    28141 tttcaccctc ttcaatcatc ccggcaacca tctccagtag ccagggggtt tcgctggtgt
    28201 cgtacgcggc aatccgaatc tgctcaatca gcacaacttc atcacgcact gggtcaaagg
    28261 gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg ccgtacctca tgactcattt
    28321 gcccgttgaa tagacgatga cgaaatctat aaagatctaa tgaaaaaaag ccgcgataaa
    28381 gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa tgtaacgggc aggttgtctg
    28441 gcttaagcat tgttaatgtc ctggcactaa tagtgaatta aatgtgaatt tcagcgacgt
    28501 ttgactgccg tttgagcagt catatgttaa attgaggcac attaacgccc tatggcacgt
    28561 aacgccaacc ttttgcggta gcggcttctg ctagaatccg caataatttt acagtttgat
    28621 cgcgctaaat actgcttcac cacaaggaat gcaaatgaag aaattgctcc ccattcttat
    28681 cggcctgagc ctttctgggt tcagttcgtt gagccaggcc gagaacctga tgcaagttta
    28741 tcagcaagca cgccttagta acccggaatt gcgtaagtct gccgccgatc gtgatgctgc
    28801 ctttgaaaaa attaatgaag cgcgcagtcc attactgcca cagctaggtt taggtgcaga
    28861 ttacacctat agcaacggct accgcgacgc gaacggcatc aactcgaacg cgaccagtgc
    28921 gtccctgcag ttaactcaat ccatttttga tatgtcgaaa tggcgtgcgt taacgctgca
    28981 ggaaaaagca gcagggattc aggacgtcac atatcagacc gatcagcaaa ccttgatcct
    29041 caacaccgcg accgcttatt tcaacgtgtt gaatgctatt gacgttcttt cctatacaca
    29101 ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc acccaacgtt ttaacgtggg
    29161 cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag tacgataccg tgctggcgaa
    29221 cgaagtgacc gcacgtaata accttgataa cgcggtagag cagctgcgcc agatcaccgg
    29281 taactactat ccggaactgg cggcgctgaa tgtcgaaaac tttaaaaccg acaaaccaca
    29341 gccggttaac gcgctgctga aagaagccga aaaacgcaac ctgtcgctgt tacaggcacg
    29401 cttgagccag gacctggcgc gcgagcaaat tcgccaggcg caggatggtc acttaccgac
    29461 tctggattta acggcttcta ccgggatttc tgacacctct tatagcggtt cgaaaacccg
    29521 tggtgccgct ggtacccagt atgacgatag caatatgggc cagaacaaag ttggcctgag
    29581 cttctcgctg ccgatttatc agggcggaat ggttaactcg cagatgaaac aggcacagta
    29641 caactttgtt ggtgccagtg agcaactgga aagcgcacat cgtagcgtcg tgcaaaccgt
    29701 acgttcctcc ttcaacaaca ttaatgcttc tatcagtagt attaacgcct acaaacaagc
    29761 cgtagtttcc gcgcaaagct cattagacgc gatggaagcg ggctactcgg tcggtacgcg
    29821 taccattgtt gatgtgttgg atgcgaccac cacgctgtac aacgccaagc aagagctggc
    29881 gaatgcgcgt tataactacc tgattaatca gctgaatatt aagtcagccc tgggtacgtt
    29941 gaacgagcag gatttgctgg cactgaacaa tgcgctgagc aaaccggttt ccactaatcc
    30001 ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt gctgatggtt atgcgcctga
    30061 tagcccggca cccgtcgttc agcaaacatc cgcacgcact accaccagta acggtcataa
    30121 ccctttccgt aactgatgac gacgacgggg cttcggcccc gtctgaacgt aaggcaacgt
    30181 aaagatacgg gttatctgcc gcattcttcc cccttctcgc ttcaatttcg accagccatc
    30241 ctctattctg atgggtattt accactggtc ccggaagaca aaaatgaaac ggacaaaatc
    30301 catacgccac gcatcgttcc gcaaaaactg gagcgcacgc catctgacac cagtcgctct
    30361 cgcggttgcc actgttttta tgctggctgg ctgtgaaaag agtgatgaaa cagtgtctct
    30421 ctatcaaaat gctgacgact gttcagctgc aaacccaggc aaaagcgccg aatgtaccac
    30481 cgcgtacaac aatgcgctga aagaagccga acgtactgcg ccgaaatacg ccacccgtga
    30541 agactgtgtt gctgaatttg gtgaaggtca gtgtcagcaa gcaccagccc aggctggcat
    30601 ggcaccagaa aaccaggcgc aggctcagca atccagcggg agtttctgga tgccgctgat
    30661 ggccggttac atgatggggc gtctgatggg cggcggcgcg ggatttgcac agcagccgct
    30721 gttctcctcg aaaaacccgg ccagtccggc ttacggtaaa tataccgacg cgacgggtaa
    30781 aaactatggc gcagcccagc caggccgcac catgaccgta ccgaagacgg caatggcacc
    30841 aaaaccggcg accaccacta ccgttacccg tggcggtttt ggtgaatctg ttgccaaaca
    30901 aagcactatg cagcgtagcg caaccggcac ctcttctcgt tcaatgggtg gctgataccg
    30961 atggaaagag tcagtattac cgagcgcccg gactggcgtg agaaagccca cgaatacggt
    31021 ttcaattttc acaccatgta cggcgagccg tactggtgtg aagatgctta ctacaagttg
    31081 accctcgccc aggttgaaaa gctggaagaa gtcaccgccg aactgcacca gatgtgcctg
    31141 aaagtggtgg aaaaagtgat cgccagcgat gagctgatga ccaaattccg cataccaaaa
    31201 cacacctgga gttttgtgcg tcaatcatgg ctgactcacc agccatcgct ttattcgcgt
    31261 cttgatctgg cgtgggatgg cactggcgaa cctaaacttc tggaaaataa cgccgatacg
    31321 ccaacgtcac tatacgaggc ggcgttcttt cagtggatct ggctggaaga tcagcttaac
    31381 gccggtaact tgccggaggg cagcgaccag tttaacagtc tgcaagaaaa actgatcgat
    31441 cgcttcgttg agctgcgtga acagtatggc ttccagttgc tgcatctcac ctgctgtcgc
    31501 gacacggtgg aagatcgcgg aaccattcag tatttgcagg actgcgcaac cgaagctgaa
    31561 atcgccaccg agttcctcta catcgacgag ataggtttag gcgaaaaagg ccagttcacg
    31621 aatttacagg atcaggtgat ttctaacctg ttcaagcttt atccgtggga atttatgctg
    31681 cgcgaaatgt tctccaccaa gctggaagat gctggcgtac gctggctgga accggcgtgg
    31741 aagagcatta tctccaacaa ggcacttcta ccgctactgt gggagatgtt cccgaatcac
    31801 ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc cgcaaatgga aaaatatgtg
    31861 gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga tcattgagaa cggcaaaacc
    31921 attgaagcag cggaaggtcc gtatggcgaa gaagggatga ttgttcagca attccacccg
    31981 ttaccgaaat tcggcgacag ctatatgctg attggtagct ggctggtgaa cgatcaacct
    32041 gccggaattg gcattcgtga agaccgtgcg ttgatcaccc aagatatgtc tcggttttat
    32101 ccacatattt ttgttgaata agccacgata ccggatggca ctcgccatcc ggtaattgtt
    32161 agcctatctg caccgacagc atacttaaac tgcccatttc tataccatcg accggaatgg
    32221 taattggctc ctgcccatcc cacacaccta acacatacaa caacggcaga tagtgctctg
    32281 gcgttgggtt cgataacgcg ccaccttcat ggtcgagata attcaccaga ggatgctgct
    32341 ctaccggccc ttgccacgtc agattcgctt tcacatactc attaaacgac gtcgcccacg
    32401 gatacggtga actatcaccg tgccacttca ctgtgcgcag gttatgcacc acgttaccgc
    32461 tggcgaccaa cattattcct tcatcacgca gcgccgcgag tttgcgcccc atttcgaaat
    32521 gccaggcggc aggtttgcta ctgtcgatac tcaactgcac catagtgata tcggcgtcag
    32581 gatacatctt aatcagcacg ccccacgagc cgtggtcaaa gccccaggct tctttatcca
    32641 gcgtcaccgg gatcggcgct aacagctcaa ccagacgctg tgccagcgca ggcgaacccg
    32701 gagcaggata atgcgtatcg tacagcgcct gcgggaagcc accaaagtca tgaatcgtgg
    32761 gcggcgtctc catcgcggtc actcctgttc cacgggtaaa ccagtgagcc gaaaccacca
    32821 caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg ccagctgcgg gtatacaaat
    32881 tatcttccag cacgttcatc ggactaccgt gacctaaaaa caatgctggc atacgtgttg
    32941 aagacatgat gatatcctta actaaaggtg tcattttgat atcctcacaa tacgcttgtt
    33001 cggcggagta agaacccgga taacaatgat gatgatcatc agttattttg acgatctgcc
    33061 tgaaggtgaa gatttataag gagttgtcga tgtcagtacc tctcattctg accatactgg
    33121 cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct cggacaaaaa ccctcgaacc
    33181 gcttactggc gttttcgcta ggttttgcgg cggggatcat gttgctcatc tcattaatgg
    33241 aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc ggtgttgggt tatggaatgt
    33301 ttatcttcgg tctgcttggc tactttggcc tggaccgcat gttgccgcat gcgcatccgc
    33361 aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc gatcaagcgc acagccattc
    33421 tgctcactct cggcatcagt ctgcataact tcccggaagg gattgccacc tttgtcacgg
    33481 cgagcagcaa cctggagctg ggatttggca tcgcactggc cgtcgcgttg cacaatatcc
    33541 ctgaaggtct ggcagtggca ggcccggttt atgcggcaac ggggtctaaa cgcaccgcga
    33601 ttctgtgggc ggggatttct ggactagcag aaattcttgg tggtgtgctg gcgtggttaa
    33661 tcctcggtag catgatttcc ccggtggtca tggcggcaat catggcggcg gttgcaggaa
    33721 ttatggtggc gctctcggtt gatgaattaa tgccgctcgc caaagagatt gaccctaata
    33781 ataaccccag ctatggcgta ctatgtggaa tgtcagtgat gggattcagt ttagtgctgc
    33841 tacaaacggc gggaattggt taaaaacaac atcagaaatt gtccagcaca tggacaactt
    33901 ctgccagctt cacgaaagcc ttattctacc ttttcggctg tctcttctct cgtactgttt
    33961 aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct ggcgaaacgc ccgacaataa
    34021 cgtttcaaat gcaggaaata acccaccaca accagagcaa tcaatacaat ccagtaccag
    34081 gcgataaaca tccttttacc cttaatattt aacaatacta aacatttagc gcataaattt
    34141 cacatatcct ttttcggata tattcatatg gtcgcataat aacggaacaa ttatatgatt
    34201 ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt ttctcataga aagagaagcg
    34261 gaattaacat cttgcatagc agggcaaaat aaaaaccgaa gccagctaca acttcggttt
    34321 cttgattaag gcagtaatta agcagcggtt ttcagctggc tttacgctca tgtgcctgac
    34381 ggtatgccac caggtcttca atagtcacga gcgccatatt gtgtttattg gcaaactcaa
    34441 tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt cagctcacac agcacaccag
    34501 ccggtttaaa gccggccagc gtcatcaaat caatagtcgc ttcagtatga ccgccacgcg
    34561 tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg gcccgggcga ttcagatcag
    34621 aaggttttgc gccatcggca atcgctgcgc gaacggtcgt aatacgatca gcggcagaaa
    34681 caccggtagt cacaccttca gccgcttcaa tggtcacggt aaaaccggtg ccataggcgc
    34741 tggtgttatt ttctaccatc attggcagat caagttgttt acggcgatct tcagtgatgc
    34801 acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc catctgctca acggtcatgg
    34861 tttctgccgg gaagatcata tcgccttcgt tttcacggtc ttcatcatca agcaccatta
    34921 caccgcgtcc ttcacgcagc gcagccagtg cattttcaac acgttcgaaa ggcgtaccaa
    34981 aagaggaaag tagcgtctga ttcatggtaa aaaaacctca ctaaaattat ggttaccaga
    35041 atcagggcag tcttaggagt ggcggcatat agccaaaata acgtgagcgg gtccatgccc
    35101 gacagaatcg ttactctctc ccatccggac tctaaccgtc ggccccggaa ttacaccgga
    35161 tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc tttcaactga gttgatttac
    35221 cgccggtggg gaatttcgcc ccgccctgag aataagcgga ttcactataa cgctaatgat
    35281 tagcggcagc aacgcatagc ttcacataat tctggtttat gacttaccct tatcgcacta
    35341 caatggtact caacacctat cagtacaggg aaactacaat gattgacccg aaaaaaattg
    35401 agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg aatcagggag ttcggggaag
    35461 atgtggagaa aaaaatccgc caaaccctac aagctcagct gacgcgcctc gaccttgtaa
    35521 gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg gactcgtgag aaactggcgc
    35581 tgcttgaaca aagaatcagc gaactggaaa atcgcagtac tgagatcaaa aagcagccag
    35641 atccagagac tctcccccca acgctgtaaa catttgtctt taatgcactg taaattatga
    35701 attaatcctc taatttacag tgttttctat tacattatcg cttaatccca ttcagagtat
    35761 catcacaaaa atgaatgatt gcaattgaaa atataattgt tatgtattct ttaagtttta
    35821 cttaatcgat a
//
LOCUS       DADLSB010000024        34769 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.024, whole genome shotgun sequence.
ACCESSION   DADLSB010000024 DADLSB010000000
VERSION     DADLSB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.024"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(18..93)
                     /locus_tag="JN331_001172"
     tRNA            complement(18..93)
                     /locus_tag="JN331_001172"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="JN331_001173"
     tRNA            complement(152..228)
                     /locus_tag="JN331_001173"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="JN331_001174"
     CDS             complement(331..1716)
                     /locus_tag="JN331_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7259370.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JN331_001175"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JN331_001175"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY7259371.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JN331_001176"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JN331_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY7259372.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVHFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JN331_001177"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JN331_001177"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY7259373.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEQ
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JN331_001178"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JN331_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY7259374.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="JN331_001179"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="JN331_001179"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAY7259375.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JN331_001180"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JN331_001180"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY7259376.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JN331_001181"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JN331_001181"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7259377.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIRKILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQCFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JN331_001182"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JN331_001182"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7259378.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQGERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JN331_001183"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JN331_001183"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY7259379.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JN331_001184"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JN331_001184"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY7259380.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JN331_001185"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JN331_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY7259381.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JN331_001186"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JN331_001186"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY7259382.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JN331_001187"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JN331_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY7259383.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JN331_001188"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JN331_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY7259384.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JN331_001189"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JN331_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY7259385.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="JN331_001190"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="JN331_001190"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY7259386.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="JN331_001191"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="JN331_001191"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY7259387.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JN331_001192"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JN331_001192"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY7259388.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="JN331_001193"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="JN331_001193"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY7259389.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="JN331_001194"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="JN331_001194"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY7259390.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGAYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     CKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23957..24847
                     /gene="ilvY"
                     /locus_tag="JN331_001195"
     CDS             23957..24847
                     /gene="ilvY"
                     /locus_tag="JN331_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY7259391.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24844..26388)
                     /gene="ilvA"
                     /locus_tag="JN331_001196"
     CDS             complement(24844..26388)
                     /gene="ilvA"
                     /locus_tag="JN331_001196"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY7259392.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26391..28241)
                     /gene="ilvD"
                     /locus_tag="JN331_001197"
     CDS             complement(26391..28241)
                     /gene="ilvD"
                     /locus_tag="JN331_001197"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY7259393.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(28306..29235)
                     /gene="ilvE"
                     /locus_tag="JN331_001198"
     CDS             complement(28306..29235)
                     /gene="ilvE"
                     /locus_tag="JN331_001198"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY7259394.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSLSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29255..29518)
                     /gene="ilvM"
                     /locus_tag="JN331_001199"
     CDS             complement(29255..29518)
                     /gene="ilvM"
                     /locus_tag="JN331_001199"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY7259395.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29515..31161)
                     /locus_tag="JN331_001200"
     CDS             complement(29515..31161)
                     /locus_tag="JN331_001200"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY7259396.1"
                     /translation="MNGAQWVVHALREQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMVEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31164..31214)
                     /locus_tag="JN331_001201"
     CDS             complement(31164..31214)
                     /locus_tag="JN331_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259397.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31300..31398)
                     /gene="ilvL"
                     /locus_tag="JN331_001202"
     CDS             complement(31300..31398)
                     /gene="ilvL"
                     /locus_tag="JN331_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY7259398.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31750..33270
                     /locus_tag="JN331_001203"
                     /pseudo
     CDS             31750..33270
                     /locus_tag="JN331_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33295..33633)
                     /gene="maoP"
                     /locus_tag="JN331_001204"
     CDS             complement(33295..33633)
                     /gene="maoP"
                     /locus_tag="JN331_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY7259399.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33752..34591
                     /gene="hdfR"
                     /locus_tag="JN331_001205"
     CDS             33752..34591
                     /gene="hdfR"
                     /locus_tag="JN331_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY7259400.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34688..34763)
                     /locus_tag="JN331_001206"
     tRNA            complement(34688..34763)
                     /locus_tag="JN331_001206"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34728..34730),aa:Trp,seq:cca)
BASE COUNT         8475 a         9516 c         8782 g         7996 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgcg
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgctgccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg aaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagtgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagct gttctccccg cacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggtcca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgca
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaagcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gcactgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa ttttgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataatacct ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtgcgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgtgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgccttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cggggtcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagctctgt gcaaaaatta accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcaacagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggt gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactggcgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gggctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatggt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccacgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacgggc gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc aggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 gccatcatac cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtgggatca gttcttcgta agcacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtgatg ttgtgcttct tattgttgcg tttgcgagat tgaattcact atatgacagg
    23881 aaatttattg cggaaattga tatattcaca acgtcatatt gcaatttctg caacgtcaac
    23941 attgagggct gcgccattgg atttacgcga tctgaaaacc ttcctgcatc tggcggaaag
    24001 ccgccatttt ggccgcagcg cgcgggcgat gcacgttagc ccgtccacgc tctcacggca
    24061 gattcagcgc ctggaagaag atctcggcca gccgctgttt gtgcgcgata accgcacggt
    24121 gacgctgact gaagcgggcg aagaactgcg cgttttcgcc cagcaaacgc tgttgcagta
    24181 tcagcagctg cgccacacca tcgaccagca agggccgtcg ctctatggcg aattacatat
    24241 cttctgctcg gtgaccgccg cttacagcca tctgccgccg attctggatc gcttccgcgc
    24301 ggaacacccg tcggtggaga tcaaactcac taccggtgat gcggcagatg caatggaaaa
    24361 agtcgtcaca ggtgaagctg atctggcgat tgccgggaag ccagagactc tgcccggcgc
    24421 ggtggcgttt tcgatgctgg agaatctggc agtggtgttg attgcacccg cattgccctg
    24481 cccggtgcgt aatcaggttt ctgtcgaaaa gccggactgg tcaacggtac cgtttatcat
    24541 ggccgatcag gggccggtgc gccgccgcat tgagttgtgg ttccgacgca ataaaatcag
    24601 taacccgatg atttacgcca cggttggcgg gcatgaagcg atggtatcga tggtggcgct
    24661 cggctgtggc gtggcattgt tgccggaagt ggtgctggaa aacagccccg aaccggtgcg
    24721 taaccgcgtg atgattttag agcgcagcga tgagaaaacg ccgtttgagc tgggcgtgtg
    24781 tgcgcagaaa aagcggctac atgagccgct aattgaggca ttctggaaga ttttgccgaa
    24841 ccattaaccc gccaaaaaga acctgaacgc cgggttattg gtttcgtcgt ggcaatcgta
    24901 gcccagctca ttcagccggg tttcgaaatc cggttcatgg tcgccaagtt cgaacgccgc
    24961 cagtacgcgc ccgtagtcgg tgccatggct gcgatagtgg aacaaagaaa tgttccagtg
    25021 cgtacccagc gtgttgagga agcgcagcag cgcgcccggt gattccggga attcgaagct
    25081 gtagaggcgt tcctgcaacg gatgtgacgg acgcccgccg accatatagc gcacatgcaa
    25141 cttcgccatt tcgtcgtcgg agagatccac cacgctgtag ccgccgtcgt tgagcatctg
    25201 caaaatttct ttgcgctctt cgaggccgcg gctcaggcgc acgccgacaa agatgcaggc
    25261 gtttttggca tcggcaaaac ggtagttgaa ctcggtgacc gatcgcccgc caagcagttg
    25321 gcagaatttg aggaagctgc ctttttcttc cggaatggtc accgccaaca acgcttcacg
    25381 ctgttcgccc agttcgcagc gttccgagac gtagcgcagg ccgtggaagt tcacgttggc
    25441 accggaaaga atatgcgcca gccgttcgcc gcgaatgttg tgctgggcga tatatttttt
    25501 cattcccgcc agcgccagcg cgccagaggg ttccgccacc gcacgcacat cttcgaataa
    25561 atccttcatc gccgcacaga tcgcatcgct atcgacggtg atgatgtcgt cgagatactc
    25621 ctggcataaa cggaaggttt cgtcaccgat gcgttttacc gcgacacctt cggcgaacag
    25681 tcccacgcgt ggcagatcaa ccggatgacc cgcatccagc gctgctttca ggcaggcgga
    25741 gtcttccgct tctacggcga tcactttgat ttgcggcatc agttgtttga tcagcaccgc
    25801 tacgcccgcc gccagaccgc cgccgccgac tggcacaaat acgcggtcga gatgggcgtc
    25861 ctgctggagc agttccagcg ccagcgtgcc ttgcccggca atcaccatcg gatgatcgaa
    25921 cggcggcacc caggtgaacc cctgctgctg tgacagttcg atcgctttag ctttcgcttc
    25981 gtcaaagttc gcgccatgaa gcagcacttc gccgccgaat ccacgtaccg catcgacttt
    26041 gatgtcggcg gtggcggttg gcataacgat cagggccttc acgcctaacc gcgcagaaga
    26101 aaacgcgacg ccctgcgcgt ggttacccgc agaagcagtg atcacgccgt gcgctttttg
    26161 ttcttccgtc aggcccgcca tcatggcgta tgcgccgcgc agcttaaagc tgtgcactgg
    26221 ctggcgatct tcgcgcttca ccagaatgac gttatcaaga cgcgacgaca gtttttccat
    26281 tttttgtagc ggcgtaacct gcgccgcctc gtaaaccggc gcgcgcagca ctgctcttaa
    26341 atattcggca ccttccggag caccggacag gggttgcgag tcagccatta ttaacccccc
    26401 agtttcgatt tatcgcgcac cgcacctttg tcggcgctgg ttgccaggct ggcgtaggcg
    26461 cgcagggcaa aggaaacctg acgttcacgg tttttcggcg tccaggcttt gtcaccccga
    26521 gcttcctgcg cttcacgacg cgccgccagt tcggcatcgc ttacctgtaa ctgaatgcca
    26581 cggttcggga tgtcgatagc gatcaggtca ccatcttcaa tcagaccaat gctgccgccg
    26641 cttgccgctt ccggtgagac gtggccgatg gaaagaccag aggtgccgcc agagaaacga
    26701 ccgtcggtga tcagcgcaca ggctttaccg agacccattg atttcaggaa gctggttggg
    26761 tagagcattt cctgcatgcc agggccgcct ttcgggcctt catagcggat caccaccaca
    26821 tcgcccgcga caactttgcc accgaggatc gcttctaccg cgtcgtcctg gctttcgtac
    26881 actttcgccg ggccggtgaa tttgaggatg ctgtcatcga cgcccgcggt tttcacgatg
    26941 cagccgtttt cagcaaaatt accgtagagc accgccaggc cgccgtcttt gctgtaggcg
    27001 tgttccagcg agcggataca gccgtttgcg cgatcgtcat cgagagaatc ccaacggcaa
    27061 tcctgcgaga atgcctgtgt agtacgaatg cccgcagggc ctgcgcggaa catatttttt
    27121 accgcgtcat cctgggtcag cataacgtcg tattgttcca gagtttgcgg caacgtcagg
    27181 ccaagtacgt ttttcacatc acggttcagt aaccccgcgc gatccagttc accgagaata
    27241 ccgataacac caccagcacg gtgaacatct tccatatggt atttttgggt gctcggcgca
    27301 actttgcata actgcgggac tttacgcgaa agtttgtcga tatcactcat ggtgaagtcg
    27361 atttccgctt cctgcgccgc cgccagcagg tgaagtaccg tgttggtaga gccgcccatc
    27421 gcgatatcca gcgtcatggc attttcaaac gccgctttgc tagcaatatt gcgcggcagt
    27481 gcactttcgt cgttttgctc gtagtaacgt ttggtcaatt caacaatgcg tttaccggca
    27541 ttgaggaaca actgcttacg gtccgcgtgg gttgccagca gcgagccgtt gcccggctgc
    27601 gacaggccca gcgcttcggt caggcagttc attgagttag cggtaaacat cccggagcag
    27661 gagccgcagg tcggacacgc ggaacgttca acctgatcgc tctgggagtc agaaactttc
    27721 gggtctgcgc cctggatcat cgcatcaacc agatcgagtt tgattatctg atcggacagt
    27781 ttggttttcc cggcctccat cgggccgccg gaaacaaaga tcaccggaat attcaggcgc
    27841 agggaagcca tcagcatccc cggggtgatt ttgtcgcagt tagagatgca gaccatggcg
    27901 tcggcgcagt gggcgttgac catatactca acggaatcag cgatcagttc gcgagatgga
    27961 agtgaataaa gcatcccccc gtggcccatg gcaatcccat catccaccgc aatggtgttg
    28021 aactctttgg caacgccgcc agccgcttca atttgttcgg cgaccagttt accgagatcg
    28081 cgcagatgga cgtgacccgg tacaaattgg gtgaacgagt tcacaaccgc gataatcggc
    28141 ttgccaaaat cggcgtcggt cattccggtg gcgcgccaca gcgcacgagc acccgccata
    28201 ttacgaccat gagtggtggt ggcggaacgg tacttaggca tactttattt actcccagtg
    28261 tctgtctcgt aaatgggacg gtgcgtgccg tcccattttt tgtatttatt gattaacttg
    28321 atctaaccag ccccatttat cttcggtttc gccagtgaag aggccgaaga aggcttgctg
    28381 aatgcgtttg gtaaccgggc cacaacggcc ttcgccaatc tgaataccgt ctacgctgcg
    28441 cactggcgtg atttctgccg ccgtaccgga cataaagact tcatccgcca ggtacaggga
    28501 ttcgcgcgac agcacctgct cacgcacttc aattcccagc tctttcgcca gtttgatgat
    28561 ggcatcacgg gtaatacccg gcagcgcgga ggaggtgaac ggtggggtga acagcacacc
    28621 atctttcact tcaaacaggt tttcgcctgc gccttcagag atataaccgt tcacatccag
    28681 cgcgatacct tcctgataac cgtggcggcg cgcttcgcta cccaccagca gggaagagag
    28741 atagttacca ccggcttttg ccgcagttgg gatggtgttt ggtgctgcgc ggttccagga
    28801 ggaaaccatc gcatcgatcc cctgctccag cgcttctgcg cccagatacg ctccccacgg
    28861 gaaagcggcg ataatcacat cggttgagta tcccgctggc gggtttactc ccatgccaac
    28921 atcaccgacg aagatcagcg gacggatata ggcgctggtg agattgtttt tgcggatcac
    28981 gtcacgacaa gcttccatca gctcatcaat gctcagtgaa accgggaagc gatagatttt
    29041 ggcggagtca tgcagacgct gcatatgctc acgatggcgg aatacaaccg gtcctttgtg
    29101 tgagtcgtag caacggatgc cttcaaaaac cgaggtgcca tagtgcagcg cgtgcgacat
    29161 cacatgcacc ttcgcgtctt cccagcgaac catctcccca ttgaaccaaa tgtaatcagc
    29221 tttcttcgtg gtcattttta tattcctttt gcgctcaggc gcggatttgt tgtgatgtgg
    29281 ttgtgctctg gcagatggca acgtgtgcga cgtccaccag tttatttaac tgactaaaca
    29341 gtaagtcgac cgaccgtggg ctggcaacgg tcaattcgat atttatattt tgtgcatcgc
    29401 tggcggcagc catattcatt gagcagacgt ggaaaccacg atgacgcacc acgcgtaaaa
    29461 cacgttctaa ggtttccgga ttgaagcgag ccgatacatt gacctgatgt tgcatcatga
    29521 taatttctcc aacatttctg aattactggc acctggcggc accagcggcc agacgttctc
    29581 aagttcgtcg attgagacat gaagcaggta tggcccatca ctgttcagca tggtgtcgag
    29641 tgccgcttca acctggtctt tacgggtgat gtgttggcca gggatgccga aggcgctggc
    29701 taacatgagg aaatcggggt tatcggtaag ggtggtttcg ctgtatcgtt cctgaaaaaa
    29761 cagttgctgc cattgtcgaa ccatccctaa ccgttggtta tcgagtaaga cgattttcaa
    29821 cggtaactgc ttgcgtttta cggtgcccag ctcttgcaca ttcatcatga aagagccgtc
    29881 accggagata cagacaacgg tatcgttcgg tcgcgcgact tgtgcgccaa ctgccgccgg
    29941 taaaccaaaa cccatggtac ctaagccgct ggaggtgatg aaattttccg ggcgagtgtg
    30001 ggcgatgtgc tgcgcagccc acatctggtg ctgccccaca tctgtggtca cgacgcaatc
    30061 cgcaggttta cgatccgaca gttgttttaa caacaacgga gcgtagatag cgtcaccggg
    30121 atggtcgtaa cgccaggaat gttcatcacg cagctgcgcg cagtgttgct gccagtcatt
    30181 gatatttaac ggctgctgta atgctggtaa cagagcattt aaatcacctt gtaatgccac
    30241 atgtgcctga cgcagcttgt tcatttctgc cgggtcgata tccatatgga taacactggc
    30301 gtgtggtgcg aaggtgttca gtttgccggt cacccggtca tcaaaacgtg cacccacggc
    30361 gatcagcagg tcacactcct gcaccgcgaa gtttgccgct ttggttccgt gcatccccag
    30421 catgcccaga tagtacggat aatctgcttc tactgcgccc agccctttca gcgtacaggt
    30481 ggcaggcatt tttgtggcag cgagaaattc acgcaaagcc ggaactgcct gcgccatccc
    30541 cacgccaccg ccaacgtaca gcatcggttt ttgcgctttt gccagcatct ggcgcgcttg
    30601 ctcgacttcg gcatgtggga aagtcacttc gttttcaacg gtggtgaacc acggttccag
    30661 atcgccgctg gctaactgga tatcttttgg gatatcgacc agaaccggac caggacgacc
    30721 tgagctggca acgtcgaatg cttcaaccat gatgcgcggc aactcttcca gagactgcac
    30781 cagaaagctg tgcttggtac aggctaacga caatcccagg acatccactt cctgaaatgc
    30841 gtcggtgccg ataaacggtg cggacacctg accagtgatg gcaacgacag ggatggaatc
    30901 taacagtgcg tccgcaagcc cggttatcag gttggttgcg cccggaccag acgtggcgat
    30961 acatacgcca gttttaccgg tagcacgggc ataaccgata gccgccattg ccgcaccctg
    31021 ctcatgtcgg cacagcaagt gctccacgcc gccgtcatac aatgcatcgt aaaccggcat
    31081 aattgcgcca cccggataac cgaaaacggt gttcacaccc tgttcccgca acgcatgtac
    31141 cacccactgt gcgccattca tagttagttc cccgtcctga atcttgagaa acagaatttt
    31201 gtgctgttat tcattgtctg ctcctcggtt atgtttttaa ggtcaaaaaa acccccggac
    31261 ctttcggtgc gggggtctta gttcgttaag gcttgatctc taagcctttc ctcgtccaag
    31321 tgcagccccg cacggtggga taataatcac caccacgcta atcacgacca ggctaatcac
    31381 tcgtagaagg gctgtcattt tgtcttttct tgcatcttgt tcgaaggaat gcctaaagag
    31441 ttaccataga ttttgcgaat agcacaagat atttttgggc ctatttttat gatggcttta
    31501 ataatttcaa tagaaattgt tgttttttaa aaagaaaata gtgagtgaaa aaattcaatt
    31561 tcagtataaa tgttgtcccc ttcctcgtag ttcatccttt gtccgctgca tttttgcgag
    31621 catcattcca ccgccagaac aactcaactt cacttgcaca aataaacagg aatcagccac
    31681 taaaattcac gaattagcgg gctacagcag cgatatctta tgcacataat ggtgactcaa
    31741 ggagggttta tgtcactgtc aattgttcat acccgcgcag ccctggaagt aaatgcgccc
    31801 ccaatcactg ttgaggtaca tatcagtaaa ggtctacccg gcttaacgat ggtgggctta
    31861 ccagaaacaa cggtgaaaga agctcgcgat cgcgtgcgca gcgccattat caatagcgga
    31921 tatgaatatc cggcgaaaaa aatcaccatc aaccttgctc cagccgatct gccaaaagaa
    31981 gggggacgat atgatttacc tatcgccatt gcgttgctgg cggcctcaga acagcttaca
    32041 gccaataagt tagatgaata tgaattagtc ggagaactag cgcttacagg cgctctgcgt
    32101 ggcgttcccg gtgcaatctc cagtgcaact gaagctatta agtcgggcag aaaaattatc
    32161 gtcgcgaaag ataacgaaga tgaagtgggg ctaattaacg gtgaaggatg cctgatagcc
    32221 gatcatctgc aggctgtctg tgcgtttctg gaaggtaaga acactctcga acgcccgaaa
    32281 ccaactgatg cagtatcccg ggcgctacaa catgatctca gtgatgttgt cggtcaggag
    32341 caaggaaaac gaggactgga aattaccgcc gctggtgggc acaacctttt actgattggg
    32401 ccgccgggaa caggtaaaac aatgctcgcc agccgtatta atggcctgtt gccagattta
    32461 agcaatgaag aggcactgga gagcgctgcg atattaagtc tggtaaatgc tgaatcagta
    32521 caaaaacaat ggcggcagcg cccgttccgc tcacctcatc acagtgcgtc gttaactgcg
    32581 atggtgggcg gtggtgcaat tccagggcca ggtgaaattt cgttggcgca taacggcgtg
    32641 ctttttcttg atgagctacc tgaatttgaa cggcgtacac tggatgcctt gcgagagccg
    32701 attgaatccg ggcagatcca tctttcacgc acacgagcaa aaataaccta tccagcccgt
    32761 ttccagcttg ttgcggcgat gaatcccagc cctaccggac attatcaggg aaaccataac
    32821 cgctgcacgc cagaacagac attacgttat ctcaaccggc tctcggggcc ctttctcgac
    32881 cgcttcgatc tctcactgga gatcccatta ccaccccccg gcattttgag taaaacggta
    32941 gtgccgggag aaagcagcgc caccgttaaa caacgcgtaa tggccgccag agagcgccaa
    33001 tttaagcggc agaataaact gaatgcctgg ctggatagtc cggaaatacg ccaattctgc
    33061 aaacttgaga gcaaagatgc gatgtggttg gaagaaacgc tgatccatct ggggttatcg
    33121 attcgtgcct agcagcggtt actgaaagtt gcacgaacca ttgctgatat tgatcagtct
    33181 gacattatca cacgtcagca tttgcaggag gcagttagct atcgtgcgat tgaccgtttg
    33241 ctcatccatc tgcaaaaact actgacataa aaaaagggca tttcgccctt tttattaatc
    33301 gtcagaatcg gtgtagtctt cagcaccttc aacctgcggt ttaccgccgg aaagggtgtg
    33361 aaaacgtttt ggacgcttaa tacgcgtcat atacttggac cacacgcgtt ctgcttctgt
    33421 cactggctca cgttcgccac ggcatactgc tacgaagagt ttctcttcct cggtaaccgg
    33481 ctcgcgtttg ccaagatcca actcattgaa ggcataacca tgacgctcaa gcagttgtgc
    33541 ctctttgatg gtgaaatcac catgacgaga gaatccacgt ggataatgtt tattgtcgaa
    33601 atatcgatta gtcgtcgtaa agctttccgc catcctgcac gctcctaatt ctttgaccga
    33661 gctagttatg gcgcggagta ttagttacgc ttgacagagt gtaaaacaaa acatttaaat
    33721 cataacgaca aataattttg cggagagcac tgtggatacg gaattgttaa aaactttcct
    33781 ggaagttagc cgaacgcgtc actttggtcg agcggctgaa tcgctctatc tgacccagtc
    33841 agcagtgagc tttcgaatca gacaactgga aaatcaactg ggtgtgaacc ttttcacccg
    33901 ccacagaaac aatatccgtt taaccgctgc cggtgaaaaa ctactgcctt atgcagaaac
    33961 gctcatgagc acctggcagg ccgcccgcaa ggaggtggcg catacctcac gacataacga
    34021 gttttctatc ggtgccagcg cctcgttgtg ggaatgtatg cttaatcagt ggttgggacg
    34081 cttgtatcaa aatcaggatg cccatacagg cttacagttc gaagcgcgaa ttgcccaacg
    34141 gcagtctctg gtaaaacagc tgcatgaacg ccagcttgat cttcttatta ccactgaagc
    34201 gcccaaaatg gacgaattta gtagtcagtt gctggggtat ttcactttag cgctttatac
    34261 cagtgcccct tctaaactaa agggagatct taactatctg cgactggagt gggggccaga
    34321 ttttcaacag catgaggcag gtttgatcgg tgctgacgaa gttcccattc tgacaaccag
    34381 ttctgctgaa ctggcacagc aacagattgc gatgcttaat ggttgcacct ggctacccgt
    34441 cagctgggcg cgtaaaaaag gcggcctgca taccgttgtc gatagcacaa cactttcacg
    34501 gccgctttat gccatatggc tgcaaaatag cgataaaaat gcgttgattc gcgatctttt
    34561 gaaaattaac gtgctggatg aagtgtatta atctgaatgg ctggcaagga tgccggtaga
    34621 aggatttact tcggagagga ttatttcaga taaaaaaaaa tccttagctt tcgctaagga
    34681 tgatttctgg caggggcgga gagactcgaa ctcccaacac ccggttttgg agaccggtgc
    34741 tctaccaatt gaactacgcc cctaattag
//
LOCUS       DADLSB010000025        34363 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.025, whole genome shotgun sequence.
ACCESSION   DADLSB010000025 DADLSB010000000
VERSION     DADLSB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.025"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..329)
                     /locus_tag="JN331_001207"
     CDS             complement(<1..329)
                     /locus_tag="JN331_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259401.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYLQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
     gene            699..1649
                     /gene="corA"
                     /locus_tag="JN331_001208"
     CDS             699..1649
                     /gene="corA"
                     /locus_tag="JN331_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY7259402.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1690..2070)
                     /locus_tag="JN331_001209"
     CDS             complement(1690..2070)
                     /locus_tag="JN331_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY7259403.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     TYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2084..2500)
                     /locus_tag="JN331_001210"
                     /pseudo
     CDS             complement(2084..2500)
                     /locus_tag="JN331_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="JN331_001211"
     CDS             complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="JN331_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY7259404.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3501..3968)
                     /locus_tag="JN331_001212"
     CDS             complement(3501..3968)
                     /locus_tag="JN331_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY7259405.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASVLDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4133..5002
                     /gene="pldA"
                     /locus_tag="JN331_001213"
     CDS             4133..5002
                     /gene="pldA"
                     /locus_tag="JN331_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY7259406.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5135..6964
                     /gene="recQ"
                     /locus_tag="JN331_001214"
     CDS             5135..6964
                     /gene="recQ"
                     /locus_tag="JN331_001214"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY7259407.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7028..7648
                     /gene="rhtC"
                     /locus_tag="JN331_001215"
     CDS             7028..7648
                     /gene="rhtC"
                     /locus_tag="JN331_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAY7259408.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7710..8330)
                     /gene="rhtB"
                     /locus_tag="JN331_001216"
     CDS             complement(7710..8330)
                     /gene="rhtB"
                     /locus_tag="JN331_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY7259409.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8441..9463
                     /gene="pldB"
                     /locus_tag="JN331_001217"
     CDS             8441..9463
                     /gene="pldB"
                     /locus_tag="JN331_001217"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY7259410.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNIRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9471..10271
                     /gene="yigL"
                     /locus_tag="JN331_001218"
     CDS             9471..10271
                     /gene="yigL"
                     /locus_tag="JN331_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY7259411.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMCFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10347..11246
                     /gene="bioP"
                     /locus_tag="JN331_001219"
     CDS             10347..11246
                     /gene="bioP"
                     /locus_tag="JN331_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY7259412.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11134..12087)
                     /gene="metR"
                     /locus_tag="JN331_001220"
     CDS             complement(11134..12087)
                     /gene="metR"
                     /locus_tag="JN331_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY7259413.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNCPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12323..14584
                     /gene="metE"
                     /locus_tag="JN331_001221"
     CDS             12323..14584
                     /gene="metE"
                     /locus_tag="JN331_001221"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY7259414.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14623..15438)
                     /locus_tag="JN331_001222"
     CDS             complement(14623..15438)
                     /locus_tag="JN331_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7259415.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYSGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15700..16461
                     /gene="udp"
                     /locus_tag="JN331_001223"
     CDS             15700..16461
                     /gene="udp"
                     /locus_tag="JN331_001223"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY7259416.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16602..18029
                     /gene="rmuC"
                     /locus_tag="JN331_001224"
     CDS             16602..18029
                     /gene="rmuC"
                     /locus_tag="JN331_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY7259417.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18124..18879
                     /gene="ubiE"
                     /locus_tag="JN331_001225"
     CDS             18124..18879
                     /gene="ubiE"
                     /locus_tag="JN331_001225"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY7259418.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18893..19498
                     /gene="ubiJ"
                     /locus_tag="JN331_001226"
     CDS             18893..19498
                     /gene="ubiJ"
                     /locus_tag="JN331_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY7259419.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19495..21135
                     /gene="ubiB"
                     /locus_tag="JN331_001227"
     CDS             19495..21135
                     /gene="ubiB"
                     /locus_tag="JN331_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY7259420.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21214..21483
                     /gene="tatA"
                     /locus_tag="JN331_001228"
     CDS             21214..21483
                     /gene="tatA"
                     /locus_tag="JN331_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY7259421.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21487..21987
                     /gene="tatB"
                     /locus_tag="JN331_001229"
     CDS             21487..21987
                     /gene="tatB"
                     /locus_tag="JN331_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY7259422.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPEKASDE
                     AHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKTAAPSP
                     SSSDKP"
     gene            21990..22766
                     /gene="tatC"
                     /locus_tag="JN331_001230"
     CDS             21990..22766
                     /gene="tatC"
                     /locus_tag="JN331_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY7259423.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22808..23590
                     /gene="tatD"
                     /locus_tag="JN331_001231"
     CDS             22808..23590
                     /gene="tatD"
                     /locus_tag="JN331_001231"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY7259424.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23587..24075)
                     /gene="rfaH"
                     /locus_tag="JN331_001232"
     CDS             complement(23587..24075)
                     /gene="rfaH"
                     /locus_tag="JN331_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY7259425.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24242..25735
                     /gene="ubiD"
                     /locus_tag="JN331_001233"
     CDS             24242..25735
                     /gene="ubiD"
                     /locus_tag="JN331_001233"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY7259426.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25781..26482
                     /gene="fre"
                     /locus_tag="JN331_001234"
     CDS             25781..26482
                     /gene="fre"
                     /locus_tag="JN331_001234"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY7259427.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEADWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26674..27837)
                     /gene="fadA"
                     /locus_tag="JN331_001235"
     CDS             complement(26674..27837)
                     /gene="fadA"
                     /locus_tag="JN331_001235"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY7259428.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWSCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGMGQGIATVFERV"
     gene            complement(27847..30036)
                     /gene="fadB"
                     /locus_tag="JN331_001236"
     CDS             complement(27847..30036)
                     /gene="fadB"
                     /locus_tag="JN331_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY7259429.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30226..31557
                     /gene="pepQ"
                     /locus_tag="JN331_001237"
     CDS             30226..31557
                     /gene="pepQ"
                     /locus_tag="JN331_001237"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY7259430.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31557..32171
                     /locus_tag="JN331_001238"
     CDS             31557..32171
                     /locus_tag="JN331_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY7259431.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32210..33661
                     /gene="trkH"
                     /locus_tag="JN331_001239"
     CDS             32210..33661
                     /gene="trkH"
                     /locus_tag="JN331_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY7259432.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAIDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33673..34218
                     /gene="hemG"
                     /locus_tag="JN331_001240"
     CDS             33673..34218
                     /gene="hemG"
                     /locus_tag="JN331_001240"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY7259433.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8075 a         8696 c         9290 g         8302 t
ORIGIN      
        1 tgctgaccgt tttcgatgta caaaccgagc ggcgcatagc tttcatcata gatgccgccg
       61 ttcatcgcca tctgcacctg accctgacta ttaatatccg ccagcagagc atgtaacgtt
      121 ccccacgctt cgccattggc tttttgcaag tacattttca cccgctctgt ttgaggatta
      181 acggtatacg cctgtacggt cagcgtcgga tctgagagtg cgcaatcatc agcggcaaca
      241 gcaaacaagg gaagtaaggt gagggtgaga aaaatccgtt tgagattcaa ggtgatcatt
      301 cctttaccaa tgagtagccg atgcgccatt ataggtcctg gatgtgggat ttttttatcc
      361 tgttagcgac cttgacgagt accaaaaagc gcgaagttca actattgttc tgtggtgttc
      421 tgttgcgtgt tgacggcaaa attttgctgg cgtaacatgc gcgcacgatc actctaagag
      481 gacattcgcc ttggacacac ccagtagata ctggctcact atcctgtcat ccaggatcaa
      541 ctcctaaggc tatccctttt tgctgatagc cttagcggtt gtcagcgacc tcaatttttc
      601 ccgtcgcgct gagtcaggct gtttaatggt ctgaaaccca atttgtttct gtgtgcccac
      661 cgaactgtcc gatattttaa gcattgggag tcccggtcat gctgagcgca tttcaactgg
      721 aaaataaccg actgacccgg ctggaagtcg aagagtcaca accccttgta aatgcagtat
      781 ggattgatct tgtcgaaccg gacgacgacg agcgactgcg cgtacaatct gaacttggcc
      841 agagcctggc aacccgcccg gaactggaag acatcgaagc atcggcacgt ttctttgaag
      901 acgacgacgg cctgcatatt cactccttct tcttctttga agatgcggaa gatcacgccg
      961 gtaactccac tgtggcattt accatccgtg atggtcgtct gtttactctg cgtgagcgtg
     1021 aactgcccgc ttttcgtctg tatcgtatgc gtgcccgtag ccagtcgatg gtagacggta
     1081 acgcctacga gttgctgctg gatctgttcg aaaccaaaat cgaacagttg gcagatgaaa
     1141 ttgaaaatat ctatagcgac ctggagcagt tgagccgggt gattatggaa gggcatcagg
     1201 gcgatgagta cgacgaggcg ctctccacgc tggcggaact ggaagatatc ggctggaaag
     1261 ttcgcctgtg tctgatggat acccagcgcg ctctcaactt cctagtgcgt aaagcgcgtt
     1321 taccgggtgg gcaactggag caggcgcgtg aaatcctgcg cgatatcgaa tccctgctgc
     1381 cgcataacga atccctgttc cagaaggtga acttcctgat gcaggcggca atgggtttta
     1441 tcaacatcga gcagaaccgc atcatcaaaa ttttctcggt ggtatccgtc gtgttcctgc
     1501 cgccaacgct ggttgcttcc agctacggga tgaactttga gtttatgcca gaactgaagt
     1561 ggagtttcgg ctaccctggc gcgattatct ttatgatcct cgcgggcctg gcaccgtatc
     1621 tgtactttaa gcggaagaac tggttgtaaa aagaaggcgg tggcttagcc aggctaagcc
     1681 tcctgttatt caaaggctcc aggtatttaa cccttttacc tctttctcat aaaaccattt
     1741 gttcgtgtta acagcaacat aggttgctac ggcaattccc agaatgttaa cgccaattaa
     1801 tgcaccaaca aatagagata gaatgccaag taatagaact ataattgctt ttttccataa
     1861 gcccagaaca aataaataga tccaggaaca gaagaaagca atgaaattca tttgaatagt
     1921 taatcgttgt cttactttta atgctttaaa tgctgcttta tattctggtg ttgctcccca
     1981 gaatccagga aagccatgtt gatcataaaa attaaatcgg tatttccatt tttcacttaa
     2041 tgaatcatcg ttcatatatt ccttactcat aaatactcca taactattgt tttgatgaat
     2101 cagtaggtgc aagcattagc atactgaaag tggaaaaata acaaatcaaa aaaatcatcg
     2161 aaccattgcc tgaacaggca aaatcttcgg ctatcattgt gatgatagag atgatatata
     2221 ctgctaatgt accaaaaaca taaatttata tatatatgaa accactatca cggagtcgtt
     2281 ggcaattcat gttgatgacg agataatgga gtgcgatggt agagactata acaagaaaac
     2341 ctgcttcccc atcgttaaaa aagataataa gaaaggcaaa aatgaaattt attaaaataa
     2401 atgaaaatat atatcggcgt ctggaaatct taccgttaga tgttgggata aacatacgta
     2461 acataaattt tacatccttg tgtgagtctc ctgtcagcat ggcaatatgc ccactctcat
     2521 acaagagtcg gcatattttt tcagaatata tttatttttt atttggacgt tctacgctgc
     2581 gtataaatcg cgtccatcac aaaaattgcc agcgccaccc aaataaaggt gaaagtcacc
     2641 atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa catcagcgtc
     2701 gggccaatgt actgaaaaaa gcctaacgtt gagagacgca ggcgcgtggc agcggcagta
     2761 aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa attcagcgac
     2821 atcgggtttt gccccatatg gctggttgaa ctgtcggcaa tagcaaacag gtaaatcgcc
     2881 gccacgggca gcagccacat ggtttcaatt aacatgccgg tttgtgcttc taccgcaatc
     2941 ttcttgcgca ccaggccgta gaaggcaaaa ctaaatgcca gccccagcgc gataataggt
     3001 agcgaaccaa aagtccacag ctggaccaac acgccacata tcgccagaat caccgccagc
     3061 cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc
     3121 gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac cgcccagata
     3181 aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa aattttctgt
     3241 ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat cagcaccacc
     3301 ataaagaaaa acgaccagat cacgcgatgc gtcaggatct catcggcggg cacgtagtaa
     3361 atcagcttga agtatgctgg cgcaataccc caaataaaat aagcggcaag agcgagtaat
     3421 acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt ggtaacagca
     3481 gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact ggcaatataa
     3541 agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac
     3601 aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc aacgcgaaga
     3661 tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat ggtttcgtgg
     3721 cgggttaagg tacttcccac gcacaccaga ccggcggcga catccagcac cgacgcaatg
     3781 actccgccgt gcaaaatgct ttgcgcccag ttacccacca tcatcggctg atttttaaag
     3841 gccagctgtg cgaactcttt ttcgtaacgc tccagttcca tccccaatgc gcggttaaat
     3901 ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc ggtcagtacg
     3961 gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat gcttctttgt
     4021 tgttggtttc tactttagga agggataact aacggcaatg gagttaagta tgtaaaatag
     4081 cccgcagaaa aacattcacc ttatcaataa ttcgttacgg agaacacgac cgatgcggac
     4141 tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg cacaagaggc
     4201 aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg ccaatatgct
     4261 gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc tcatctacac
     4321 ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg aaaatgcgcg
     4381 taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg ggattttagg
     4441 cccaaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag
     4501 cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc
     4561 caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt ataaccacga
     4621 ctctaacggg cgttcagacc cgacctcccg cagctggaac cgcctttata ctcgcctgat
     4681 ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg gtaatactga
     4741 cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg gctatcacct
     4801 cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct acggcggcgc
     4861 ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc aggtttacag
     4921 cggctatggc gaatcgctca tcgactataa cttcaaccag acccgtgtcg gtgtaggggt
     4981 tatgctaaac gacttgtttt gatgaacggt tgagtggttg gcaaatctgg aatccagcat
     5041 ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata
     5101 gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag tgttgaatct
     5161 ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg
     5221 ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggttg ttatgcccac
     5281 tggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg gccttaccgt
     5341 ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactac aggccaacgg
     5401 cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg aagtgatgac
     5461 aggctgccgc accgggcaaa ttcgcttact gtatatcgcc ccggaacgcc tgatgctgga
     5521 taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgtcg atgaagcgca
     5581 ctgtatctca caatggggcc acgatttccg cccggaatat gccgcgctcg gtcagttgcg
     5641 ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg acaccacgcg
     5701 ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga
     5761 ccgtccgaat attcgctaca tgctgatgga gaaattcaaa ccgctcgatc agttgatgcg
     5821 ctacgtgcag gaacagcgcg gtaagtctgg cattatctac tgcaacagcc gggcgaaagt
     5881 agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct atcatgccgg
     5941 gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg atctgcaaat
     6001 tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc gcttcgtggt
     6061 ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc gcgccgggcg
     6121 tgatggcctg cctgcggaag cgatgctgtt ttacgatccg gctgatatgg cgtggctgcg
     6181 ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgagcgcc acaaactcaa
     6241 tgcgatgggc gcgtttgccg aagcgcaaac ttgccgtcgt ctggtattgc tgaactattt
     6301 tggcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca
     6361 gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg tgaatcagcg
     6421 gtttgggatg ggttatgtgg tggaagtgat tcgtggtgct aataaccagc gtatccgcga
     6481 ctatggtcat gacaaactga aagtctatgg tatgggccgt gataaaagcc atgaacattg
     6541 ggtgagcgtg atccgccagc tgattcatct cggcctggtg acgcaaaata ttgcccagca
     6601 ttctgcccta caactgacag aggccgcgcg cccggtgctg cgcggcgaat cctctttgca
     6661 acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg
     6721 caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga
     6781 aagtaatgtc ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga tggctgaaca
     6841 gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc gcaagctgga
     6901 acgctttggt aaaccgttta tggcgctgat tcgtgcgcat gtcgatggcg acgacgaaga
     6961 gtagtcagca gcataaaaaa gtgccagtat gaagactccg taaacgtttc ccccgcgagt
     7021 caaatgtatg ttgatgttat ttctcaccgt cgccatggtg cacattgtgg cgcttatgag
     7081 ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc gtaaagaagc
     7141 gatgatgggc gtgctgggca ttacctgcgg cgtaatggtt tgggctggga ttgcgttgct
     7201 tggcctgcat ttgattatcg aaaaaatggc ctggctgcat acgctgatta tggtgggcgg
     7261 tggcctgtat ctctgctgga tgggttacca gatgctacgc ggtgcactga aaaaagaggc
     7321 tgtatctgcg cctgcaccac aggttgagct ggcgaaaagc gggcgcagtt tcctgaaagg
     7381 cttactgacc aatctcgcca atccgaaagc gattatctat tttggttcgg tattctcatt
     7441 gtttgtcggc gataacgttg gcactaccga gcgctggggc attttcgcgt tgattattgt
     7501 cgaaacactg gcgtggttta ccgtcgttgc cagtctgttt gccctgccgc aaatgcgccg
     7561 tggttatcaa cgtctggcga agtggattga tggttttgcc ggggcgttat ttgccggatt
     7621 tggcattcat ttgattattt cgcggtgatg ccagacgcgt cttcagagta agtcggataa
     7681 ggcgtttacg ccgcatccga cattattttt cacgcatgcc tcgccgatgc taacagcgct
     7741 cccaccagca taaacaacga gccgaaaatc ttattcagcg ccttcatctg ctttggtcct
     7801 ttaatccata gagcaatcct ttgagcaagg gtggcgtaac cgatcatcac aataatatcg
     7861 accacaatag tggtgacgcc gagcacgata tactgcatca gttgcggctg ttgcggcatg
     7921 atgaattgcg gaaatagcgc cgccagaaac acaatacttt tgggattggt gagattcaca
     7981 aaaactgcgc gctggaacaa atgtcgacgc gattgagtag aggccagcga tttaagatca
     8041 attgcgccag cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc cgcgcctgcc
     8101 cactttaaca cttcaaacgc aatcactgag cgggaaaata gcgtccccaa ccccacgcca
     8161 accagcacaa tatgaatcgc cagtccggtc tgaagcccag caatagacgc caccgcgccg
     8221 cgataaccgt ggttgagcga ggtggtcata gtgttgattg caccagagcc tggcgacagg
     8281 cttaaaatga tcgatgtcag caggtaggca aaccaccatt ctaaggtcat gatgaactcc
     8341 cggtgtgtct atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc aggcacgctt
     8401 aaaaaaacga ttttacgtgg tttaagaggc agattacccg atgtttcagc agcaaaaaga
     8461 ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg ggaccgctga ctgatttctg
     8521 gcgccagcgt gatgaagcag agtttactgg tgtggatgac attccggtgc gctttgtccg
     8581 ttttcgcgca cagcaccatg accgggtggt agtcatctgc ccggggcgta ttgagagcta
     8641 cgtaaaatat gcggaactgg cctacgacct gttccatttg gggtttgatg tcttaatcat
     8701 cgaccatcgc gggcagggac gttccggtcg cctgttagcc gatccgcatc tcgggcatgt
     8761 taatcgcttt aatgattatg ttgatgatct ggcggcattc tggcagcagg aggttcagcc
     8821 cggtccgtgg cgtaaacgct atatactggc acattcgatg ggcggtgcga tctccacatt
     8881 atttctgcaa cgccatccag gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg
     8941 gatcgtgatt cgtatgccgt catttatggc acggcagatc ctcaactggg ccgaagcgca
     9001 tccacgtttc cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc
     9061 tatcaacgta ctgacccaca gcagacagcg atatcgacgt aacatacgct tctatgctga
     9121 tgacccaacg attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa gtattctggc
     9181 tggcgaacag gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc tcttacaagc
     9241 tgaagaggaa cgcgtggtgg ataaccgcat gcacgaccgt ttttgtgaac tccgcaccgc
     9301 cgcgggccat cctgtcgaag gaggacggcc gttggtaata aaaggtgctt accatgagat
     9361 cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac gccatcgttg attttttcaa
     9421 caggcataac tcacccagcg gaaaccgctc tacagaggtt taaatttctt atgtaccagg
     9481 ttgttgcgtc tgatttagat ggcacgttac tttctcccga ccatacgtta tccccttacg
     9541 ccaaagaaac tctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc
     9601 gtcaccacgt tgatgtgggg caaattcgcg ataatctgga gattaagtct tacatgatta
     9661 cctccaatgg tgcgcgcgtt cacgatctgg atggcaatct gatttttgct cataacctgg
     9721 atcgcgacat tgccagcgat ctgtttggcg tagtcaacga caatccggac atcattacta
     9781 acgtttatcg cgacgacgaa tggtttatga atcgccatcg cccggaagag atgtgctttt
     9841 ttaaagaagc ggtgttcaaa tatgcgctgt atgagccggg attactggag ccggaaggcg
     9901 tcagcaaagt gtttttcacc tgcgattccc atgaacaact gctgccgctg gagcaggcga
     9961 ttaacgctcg ctggggcgat cgcgtcaacg tcagtttctc taccttaacc tgtctggaag
    10021 tgatggcggg cggcgtgtcg aaaggccatg cgctggaagc agtggcgaag aaactgggct
    10081 acagcctgaa ggattgtatt gcgtttggtg acgggatgaa cgacgccgaa atgctgtcga
    10141 tggcggggaa aggctgcatt atgggcagtg cgcaccagcg tctgaaagat ctgcatccgg
    10201 agctggaagt tattggtact aatgccgacg acgcggtgcc gcattatctg cgtaaactct
    10261 atttatcgta atcgttcttt atttggtcag ttgtcaacct gatacttcgc tacaatggat
    10321 actcgttaat caaagagttt tccattgtgg cgctacttat catcaccacg attctgtggg
    10381 ccttctcctt tagcttttat ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg
    10441 tgctggtgcg cgttggcctg gcggcactcg tttttctgcc gtttctgcgt acccgtggca
    10501 atagcctgaa aacggtcggc ctgtatatgc tggtgggcgc gatgcagctc ggcgtgatgt
    10561 atatgctgag tttccgcgct tatctctacc tgacggtctc cgaattgttg ctgttcaccg
    10621 tactgacgcc gctctacatc acgctgattt atgacatcat gagtaagcgc cgtctgcgct
    10681 ggggctatgc ttttagcgcc ttgctggcgg tgattggtgc cgggattatt cgctatgatc
    10741 aggtcaccga ccatttctgg actggcttgt tgctggtgca actctccaat atcacttttg
    10801 ccatcggcat ggtgggttac aaacgcctga tggaaactcg cccgatgccg cagcataacg
    10861 cctttgcgtg gttctatctt ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg
    10921 gaaatgcgca gaaaatgccg caaaccacgt tgcaatgggg cattctggtg tttcttggcg
    10981 tggtggcttc cgggattggc tactttatgt ggaactacgg cgcgacgcag gtggacgccg
    11041 gaacgctggg cattatgaat aatatgcacg ttccggcagg gctgctggta aacctggcta
    11101 tctggcacca acagccgcac tggccaacgt ttattacagg cgcgctggtg atcctggcct
    11161 cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca gatgatcgca
    11221 ggcgtgattg cgcgctgagc gaataaacgc ttccgtaact ggctgacgct gctcgccatc
    11281 gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc agggttttgg tcaccaccag
    11341 accctggcgc tcaaaactct ctactaccca atgcggtagc gcggcaatac ccatccgcgc
    11401 ggcaaccatt tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc
    11461 tgccggctga agaaaatgcc gccagacatc cagtcgacta cgctgcaccg gataaattaa
    11521 tagcgtctcg ctggcgagat cttccggtgt aatacgcgtt tttgccgcca gtggatggtc
    11581 aggtgctaac accaggcgta cttcataatc gaacatcggc gaataatgca ggccactgcg
    11641 cggcaggata tcggaggtca tcaccagatc cagctcgccc tgttgcaacg cgggctgcgg
    11701 gtcaaacgtc acgcccgatt taaaatccat ctctacctgc gggcagttct tatggaaatt
    11761 ttctaaagcg ggtgtcagcc actgaataca gctatggcac tcaatggcaa tgcgcagacg
    11821 cgtctgctgc ggttcattgc aggcttgcag tgcctggctg atttgcggca gcacctggtt
    11881 tgccagttgc aacaggattt ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac
    11941 aaatagccgg aagccaaggc gttgttccag atcgctaaac tggtgagaca gggcggattg
    12001 cgtctgatgc aacgtcgccg cagcggctgc gagcgagccg cagttccgca acgcttgtag
    12061 cgttttcagg tgttttactt cgatcatgaa agtccttcac ttcggcatga ataatttgcg
    12121 cttgaggaat atacagtaac cgccaattat ggatgtgtaa acatctggac ggctaaaatc
    12181 cttcgtcttt taaatttatg gtgcgttggc tgcgtttctc caccccggtc acttacttca
    12241 gtaagctccc ggcgatgaat aaacttgccg ccttccctaa attcaaaatc cataggattt
    12301 acatataatt agaggaagaa aaatgacaat attgaatcac accctcggtt tccctcgcgt
    12361 tggcctgcgt cgcgagctga aaaaagcgca agaaagttat tgggcgggga actccacgcg
    12421 tgaagaactg ctgacagtag ggcgtgaact gcgtgctcgt cactgggatc aacaaaagca
    12481 agcgggtatc gacctgctgc cggtgggcga ttttgcctgg tacgatcatg tactgaccac
    12541 cagtttgctg ctgggtaatg ttccgccacg tcatcagaac aaagacggtt cggtagatat
    12601 cgacaccctg ttccgtattg gtcgtggacg tgcgccgact ggcgaacctg cggcggcagc
    12661 ggaaatgacc aaatggttta acaccaacta tcactacatg gtgccggagt tcgttaaagg
    12721 ccaacagttc aaactgacct ggacgcagct gctggaggaa gtggacgagg cgctggcgct
    12781 gggccacaac gttaagcctg tgctgctggg gccggttact tacctgtggc tggggaaagt
    12841 gaaaggtgaa cagtttgacc gccttagcct gctgaacgac attctgccgg tttatcagca
    12901 agtgctggca gaactggcga aacgcggcat cgagtgggta cagattgatg aacccgcgct
    12961 ggtactggaa ctaccacagg cgtggctgga cgcatacaaa cccgcttacg acgcgctcca
    13021 gggacaggtg aaactgctgc tgaccaccta ttttgaaggc gtaacgccaa atctcgacac
    13081 gattactgcg ctgcctgttc aggggctgca tgtggacctt gtacatggta aagatgacgt
    13141 tgttgaactg cacaagcgcc tgccttctga ctggctgctg tctgcgggtc tgatcaatgg
    13201 tcgtaacgtc tggcgcgccg atcttactga gaaatatgcg caaattaagg acattgtcgg
    13261 caaacgtgat ttgtgggtgg catcttcctg ctcgttgctg cacagcccca tcgacctgag
    13321 cgtggaaacg cgtcttgatg cagaagtgaa aagctggttt gccttcgccc tacaaaaatg
    13381 ccatgaactg gcactgctgc gcgatgcgct gaacagtggt gacacggcag ctctggcaga
    13441 gtggagcgcc ccaattcagg cgcgtcgtca ctctactcgt gtacataatc cggcggtaga
    13501 aaagcgtctg gcggcgatca ccgcccagga cagccagcgt gcgaatgtct atgaagtgcg
    13561 tgctgaagcc cagcgtgcgc gttttaaact gccagcgtgg ccgaccacca cgattggttc
    13621 cttcccgcaa accacggaaa ttcgtaccct gcgtctggat ttcaaaaagg gcaatctcga
    13681 cgccaacaac taccgcacgg gcattgcgga acatatcaag caggccattg ttgagcagga
    13741 acgtttggga ctggatgtgc tggtacatgg cgaggccgag cgtaatgaca tggtggaata
    13801 ctttggcgag cacctcgacg gatttgtctt tacgcaaaac ggttgggtac agagctacgg
    13861 ttcccgctgc gtgaagccac cgattgtcat tggtgacatt agccgcccgg caccgattac
    13921 cgtggagtgg gcgaagtatg cgcaatcgct gaccgacaaa ccggtgaaag ggatgctgac
    13981 ggggccggtg accatactct gctggtcgtt cccgcgtgaa gatgtcagcc gtgaaaccat
    14041 cgccaaacag attgcgctgg cgctgcgtga tgaagtggcc gatctggaag ccgctggaat
    14101 tggcatcatc cagattgacg aaccggcgct gcgcgaaggt ttaccgctgc gtcgtagcga
    14161 ctgggatgcg tatctccagt ggggcgtaga ggccttccgt atcaacgccg ccgtggcgaa
    14221 agatgacaca caaatccaca ctcacatgtg ttattgcgag ttcaacgaca tcatggattc
    14281 gattgcggcg ctggacgcag acgtcatcac catcgaaacc tcgcgttccg acatggagtt
    14341 gctggagtcg tttgaagagt ttgattatcc aaatgaaatc ggtcctggcg tctatgacat
    14401 tcactcgcca aacgtaccga gcgtggaatg gattgaagcc ttgctgaaga aagcggcaaa
    14461 acgcattccg gcagagcgcc tgtgggtcaa cccggactgt ggcctgaaaa cgcgcggctg
    14521 gccagaaacc cgcgcggcac tggcgaacat ggtgcaggcg gcgcagaatt tgcgtcgggg
    14581 gtaaatccaa accgggtggt aataccaccc ggtcttttct cattacagcg acttcttccc
    14641 accatactgc ttaaaccatt ccagcatacg ctgccagcca tctttcgcag attcggcatg
    14701 atagctcggg cgataatcag cgttgaatgc atgcccggca tccgggtaca cgataatctc
    14761 tgctttcgca ttagcagccc gcagcgcctg gcgcatcgtt tcaacgctct cctgcggaat
    14821 gctgttatcc tgaccactat ataagccgag aaccggcgcg ttaagatcgg ttgcgatatc
    14881 aacaggttgt ttcggtgaat tcagcgactt gtcgcccgtc agtttgccgt accacgccac
    14941 tgcggctttt agctgtggat tatgcgcggc atacagccag gtgatacgtc caccccagca
    15001 gaatccggtg atcattaaac gatgaacatc gccgccgttg cgggaagccc aactggcgac
    15061 atgatcgaga tcggccagca cctgcgagtc aggcactttt gctaccagac cgctaagcaa
    15121 cgtggggata tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt caggtgcgat
    15181 agccagatac ccctccagcg ccagacggcg acaaatgtcg cggatatgtt catgcacgcc
    15241 aaaaatttcc tgcactacaa tgaccactgg cagtgggcca tcgctttgct ttggtctggc
    15301 atggtaagca ggcatgttat ccccttgtga agggatagag gtgaagcccg ccacaatcgc
    15361 gtcgtccggg gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg caaatccaga
    15421 ttgttgtgtt gttgccatgg tattctccgt acccttataa aaatgttgcg caatgttaac
    15481 tatagtcagc atgcaacaaa tcacattgcc tgaatcggct catcttttat gcagtcctgc
    15541 agaatgaaga gtgatttatg tgatatgaat cacttttggt gggtaaattt atgcaacgca
    15601 tttgcgtcat ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt
    15661 tgcttcttct gactaaaccg attcacagag gagttgtata tgtccaagtc tgatgttttt
    15721 catctcggcc tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt ccctggcgac
    15781 ccggatcgtg tggaaaagat cgccgcgctg atggataagc cggttaagct ggcatctcac
    15841 cgcgaattca ctacctggcg tgcagagctg gatggtaaac ctgttatcgt ctgctctacc
    15901 ggtatcggcg gcccgtctac ctctattgct gttgaagagc tggcacagct gggcattcgc
    15961 accttcctgc gtatcggtac aacgggcgct attcagccgc atattaatgt gggtgatgtc
    16021 ctggttacca cggcgtctgt ccgtctggat ggcgcgagcc tgcacttcgc accgctggaa
    16081 ttcccggctg tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc gaaatctatt
    16141 ggcgcgacaa ctcacgttgg cgtgacagct tcttctgata ccttctaccc aggtcaggaa
    16201 cgttacgata cttactctgg tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg
    16261 caggcgatgg gcgtaatgaa ctatgaaatg gaatctgcaa ctctgctgac catgtgtgca
    16321 agtcagggcc tgcgtgccgg tatggtagcg ggtgttatcg ttaaccgcac ccagcaagag
    16381 atcccgaatg ctgagacgat gaaacaaacc gaaagccatg cggtgaaaat cgtggtggaa
    16441 gcggcgcgtc gtctgctgta attctcttct cctgtctgaa ggccgacgcg ttcggccttt
    16501 tgtatttttg cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt gtacagcaca
    16561 attctatttt gtgcgggtaa gttgttgcgt caggaggcgt tgtggatttc tcaatcatgg
    16621 tttacgcagt tattgcgttg gtgggtgtgg caattggctg gctgtttgcc agttatcaac
    16681 atgcgcagca aaaagccgag caattagctg aacgtgaaga gatggtcgcg gagttaagcg
    16741 cggcaaaaca acaaattacc caaagcgagc actggcgtgc agagtgtgaa ttgctcaata
    16801 acgaagtgcg cagcctgcaa agtattaaca cctctctgga ggccgatctg cgtgaagtaa
    16861 ccacgcggat ggaagccgca cagcaacatg ctgacgataa aattcgccag atgattaaca
    16921 gcgagcagcg cctcagtgag cagtttgaaa acctcgccaa ccgtattttt gagcacagca
    16981 atcgccgggt tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg ccgctacgtg
    17041 aacaactgga cggtttccgc cgtcaggttc aggacagctt cggtaaagaa gcacaagaac
    17101 gccataccct gacccacgaa attcgcaatc tccagcaact caacgcgcaa atggcccagg
    17161 aagcgatcaa cctgacgcgc gcgctgaaag gcgacaataa aacccagggc aactggggcg
    17221 aggtagtatt gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat gaatatgaaa
    17281 cccaggtcag catcgaaaat gacgcccgct cgcggatgca gccggatgtc atcgtgcggc
    17341 tgccgcaggg aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc tatgaacgct
    17401 attttaacgc cgaagacgac tacacccgcg aaagcgcgct acaggaacat atcgcgtcgg
    17461 tgcgtaacca tatccgtttg ctgggacgca aagattatca acagctgccg gggctgcgaa
    17521 ctctggatta cgtgctgatg tttattcccg ttgaacccgc ttttttactg gcgcttgacc
    17581 gccagccgga gctgatcacc gaagcgttga aaaacaatat catgctggtt agcccgacta
    17641 cgctgctggt ggcgttgcgt actattgcca acctgtggcg ttatgagcat caaagccgta
    17701 acgcccagca aatcgccgat cgtgccagca agctgtacga caagatgcgt ttgttcatcg
    17761 atgacatgtc cgcgattggt caaagtctcg acaaagcgca ggataactac cgccaggcaa
    17821 tgaaaaaact ctcttcaggt cgcggaaatg tgctggcgca ggcagaagcg tttcgcggtt
    17881 taggagtaga aattaaacgc gagattaatc cggatttggc tgaacaggcg gtgagccagg
    17941 atgaagagta tcgacttcgg tcggttccgg agcagccgaa tgatgaagct tatcaacgcg
    18001 atgatgaata taatcagcag tcgcgctagc ccattagggg tagttaagcc gggtagaaat
    18061 ctagggcatc gacgcccaat ctgttacact tctggaacaa ttttttgatg agcaggcatt
    18121 gagatggtgg ataagtcaca agaaacgacg cactttggtt ttcagaccgt cgcgaaggaa
    18181 caaaaagcgg atatggtcgc ccacgttttc cattccgtgg catcaaaata cgatgtcatg
    18241 aatgatttga tgtcatttgg tattcatcgt ttgtggaagc gattcacgat tgattgcagc
    18301 ggcgtacgcc gtgggcagac cgtgctggat ctggctggtg gcaccggcga cctgacagcg
    18361 aaattctccc gcctggtcgg agaaactggc aaagtggtcc ttgctgatat caatgaatcc
    18421 atgctcaaaa tgggacgcga gaagctgcgt aatatcggtg tgattggcaa cgttgagtat
    18481 gttcaggcga acgctgaggc gctgccgttc ccggataaca cctttgattg catcaccatt
    18541 tcgtttggtc tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat gtatcgcgtg
    18601 ctgaaacccg gcggccgcct gctggtgctt gagttctcga agccaattat cgagccgctg
    18661 agcaaagcct atgatgcata ctccttccat gtgctgccgc gtattggctc actggtcgcg
    18721 aacgacgccg acagctaccg ttatctggca gaatccatcc gtatgcatcc cgatcaggat
    18781 accctgaaag ccatgatgca ggatgccgga ttcgaaagtg tcgactacta caatctgacg
    18841 gcaggggttg tggcgctgca tcgtggttat aagttctgac aggagaccgg aaatgccttt
    18901 taaaccttta gtgacggcag gaattgaaag tctgctcaac accttcctgt atcgctcacc
    18961 cgcgctgaaa acagcccgct cgcgtctgct gggtaaagta ttgcgcgtgg aggtaaaagg
    19021 cttttcgacg tcattgattc tggtgttcag cgaacgccag gttgatgtac tgggcgaatg
    19081 ggcaggcgat gctgactgca ccgttatcgc ctacgccagt gtgttgccga aacttcgcga
    19141 tcgccagcag cttaccgcac tgattcgcag tggtgagctg gaagtgcagg gcgatattca
    19201 ggtggtgcaa aacttcgttg cgctggcaga tctggcagag ttcgaccctg cggaactgct
    19261 ggccccttat accggtgata tcgccgctga aggaatcagc aaagccatgc gcggaggcgc
    19321 aaagttcctg catcacggca ttaagcgcca gcaacgttat gtggcggaag ccattactga
    19381 agagtggcgt atggcacccg gtccgcttga agtggcctgg tttgcggaag agacggctgc
    19441 cgtcgagcgt gctgttgatg ccctgaccaa acggctggaa aaactggagg ctaaatgacg
    19501 ccaggtgaag tacggcgcct atatttcatc attcgcactt ttttaagcta cggacttgat
    19561 gaactgatcc ccaaaatgcg tatcaccctg ccgctacggc tatggcgata ctcattattc
    19621 tggatgccaa atcggcataa agacaaactt ttaggtgagc gactacgact ggccctgcaa
    19681 gaactggggc cggtttggat caagttcggg caaatgttat caacccgccg cgatcttttt
    19741 ccaccgcata ttgccgatca gctggcgtta ttgcaggaca aagttgctcc gtttgatggc
    19801 aagctggcga agcagcagat tgaagctgca atgggcggct tgccggtaga agcgtggttt
    19861 gacgattttg aaatcaagcc gctggcttct gcttctatcg cccaggttca taccgcgcga
    19921 ttgaaatcga atggtaaaga ggtggtgatt aaagtcatcc gcccggatat tttgccggtc
    19981 attaaagcgg atctgaaact tatctaccgt ctggctcgct gggtgccgcg tttgctgccg
    20041 gatggtcgcc gtctgcgccc aaccgaagtg gtgcgcgagt acgaaaagac cttgattgat
    20101 gaactgaatt tgctgcggga atctgccaac gccattcagc ttcggcgcaa ttttgaagac
    20161 agcccgatgc tctacatccc ggaagtttac cctgactatt gtagtgaagg gatgatggtg
    20221 atggagcgca tttacggcat tccggtgtct gatgttgcgg cgctggagaa aaacggcact
    20281 aacatgaaat tgctggcgga acgcggcgtg caggtgttct tcactcaggt ctttcgcgac
    20341 agctttttcc atgccgatat gcaccctggc aacatcttcg taagctatga acacccggaa
    20401 aacccgaaat atatcggcat tgattgcggg attgttggct cgctaaacaa agaagataaa
    20461 cgctatctgg cagaaaactt tatcgccttc tttaatcgcg actatcgcaa agtggcagag
    20521 ctacacgtcg attctggctg ggtgccacca gataccaacg ttgaagagtt cgaatttgcc
    20581 attcgtacgg tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc gtttggacat
    20641 gtactgttaa atctgtttaa tacggcgcgt cgcttcaata tggaagtgca gccgcaactg
    20701 gtgttactcc agaaaaccct gctctatgtc gaaggggtag gacgccagct ttatccgcag
    20761 ctcgatttat ggaaaacagc gaagcctttc ctggagtcgt ggattaaaga tcaggtcggt
    20821 attcctgccc tggtgagagc atttaaagaa aaagcgccgt tctgggtcga aaaaatgcca
    20881 gaactgcctg aactggttta cgacagtttg cgccagggca agtatttaca gcacagtgtt
    20941 gataagattg cccgcgagct tcagtcaaat catgtacgtc agggacaatc gcgttatttt
    21001 ctcggaattg gcgctacgtt agtattaagt ggcacattct tgttggtcag ccgacctgag
    21061 tgggggctga tgcccggctg gttaatggca ggtggtctga tcgcctggtt tgtcggttgg
    21121 cgcaaaacac gctgattttt tcatcgctca aggcgggccg tgtaacgtat aatgcggctt
    21181 tgtttaatca tcatctacca cagaggaaca tgtatgggtg gtatcagtat ttggcagtta
    21241 ttgattattg ccgtcatcgt tgtactgctt tttggcacca aaaagctcgg ctccatcggt
    21301 tccgatcttg gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga tgaaccaaag
    21361 caggataaaa ccagtcagga tgctgatttt actgcgaaaa ctatcgccga taagcaggcg
    21421 gatacgaatc aggaacaggc taaaacagaa gacgcgaagc gccacgataa agagcaggtg
    21481 taatccgtgt ttgatatcgg ttttagcgaa ctgctattgg tgttcatcat cggcctcgtc
    21541 gttctggggc cgcaacgact gcctgtggcg gtaaaaacgg tagcgggctg gattcgcgcg
    21601 ttgcgttcac tggcgacaac ggtgcagaac gaactgaccc aggagtttca ggacagtctg
    21661 aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg aactgaaagc gtcgatggat
    21721 gaactacgcc aggccgcgga gtcgatgaag cgttcctacg ttgcaaacga tcctgaaaag
    21781 gcgagcgatg aagcgcacac catccataac ccggtggtga aagataatga agctgcgcat
    21841 gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt cgccggaaca gaagccagaa
    21901 accacgccag agccggtggt aaaacctgct gcggacgctg aaccgaaaac cgctgcccct
    21961 tccccttcgt cgagtgataa accgtaaaca tgtctgtaga agatactcaa ccgcttatca
    22021 cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat tatcgcggtg atcgtgatat
    22081 tcctgtgtct ggtctatttc gccaatgaca tctatcacct ggtatccgcg ccattgatca
    22141 agcagttgcc gcaaggttca acgatgatcg ccaccgacgt ggcctcgccg ttctttacgc
    22201 cgatcaagct gacctttatg gtgtcgctga ttctgtcagc gccggtgatt ctctatcagg
    22261 tgtgggcatt tatcgcccca gcgctgtata agcatgaacg tcgcctggtg gtgccgctgc
    22321 tggtttccag ctctctgctg ttttatatcg gcatggcatt cgcctacttt gtggtctttc
    22381 cgctggcatt tggcttcctt gccaataccg cgccggaagg ggtgcaggta tccaccgaca
    22441 tcgccagcta tttaagcttc gttatggcgc tgtttatggc gtttggtgtc tcctttgaag
    22501 tgccggtggc aattgtgctg ctgtgctgga tggggattac ctcgccagaa gacttacgca
    22561 aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg gatgttgctg acgccgccgg
    22621 atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg tctgtttgaa atcggtgtct
    22681 tcttctcacg cttttacgtt ggtaaagggc gaaatcggga agaggaaaac gacgctgaag
    22741 cagaaagcga aaaaactgaa gaataaattc aaccgcccgt cagggcggtt gtcatatgga
    22801 gtacaggatg tttgatatcg gcgttaattt gaccagttcg caatttgcga aagaccgtga
    22861 tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg ctactcatca ccggcactaa
    22921 cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag tattcgtcct gttggtcaac
    22981 ggcgggcgta catcctcacg acagcagcca gtggcaagct gcgactgaag aagcgattat
    23041 tgagctggcc gcgcagccag aagtggtggc gattggtgaa tgtggtctcg actttaaccg
    23101 caacttttcg acgccggaag agcaggaacg cgcttttgtt gctcagctac gcattgccgc
    23161 agaattaaac atgccggtat ttatgcactg tcgcgatgcc cacgagcggt ttatgacatt
    23221 gctggagccg tggctggata aactgcctgg tgcggttctt cattgcttta ccggcacacg
    23281 cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc ggcattaccg gttgggtttg
    23341 cgatgaacga cgcgggctgg agctgcggga attgttgccg ttgattccgg cggagaaatt
    23401 gctgatcgaa actgatgcgc cgtatctgct cccgcgcgat ctcacgccaa agccatcatc
    23461 ccggcgcaac gagccagccc atctgcccca tattttgcaa cgtattgcgc actggcgtgg
    23521 agaagatgcc gcatggctgg ctgccaccac ggatgccaat gtcaaaacac tgtttgggat
    23581 tgcgttttag agtttgcgga actcggtgtt cttcacactg tgcttaatct ctttattaat
    23641 aagattaagc aatagcatgg agcgagcctc accatcgggt tcggtgaaaa tggcctgaaa
    23701 gccttcgaac gcgccttcgg taataatcac cttatcaccc ggataagggg gtgccggatc
    23761 gacaatgtct ttcggtttat ataccgatag ctgatgaatc accgccgatg ggactatcgc
    23821 tggcgacgcg ccaaagcgca caaagtggct gacgccacgc gtagcgctga tggtcgtggt
    23881 atgaatcact tctggatcaa attccacaaa caggtagtta gggaataacg gttcactgac
    23941 tgcggtacgt ttaccccgta cgattttttc cagggtgatc atcggagcga ggcaattaac
    24001 cgcctgtctc tcaaggtgtt cctgggcacg ttgaagttgc ccgcgcttgc agtacagtaa
    24061 ataccaggat tgcataatga ctcttatccg tttaatcggg gcgcaaggat agcaaaagct
    24121 ttacgctaag ttaattatat tccccggttt gcgttatacc gtcagagttc acgctaattt
    24181 aacaaattta cagcattgca aagatgaacg ccgtataatg ggcgcagatt aagaggctac
    24241 aatggacgcc atgaaatata acgatttacg cgacttcctg acgctgcttg aacagcaggg
    24301 tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg gaaatcactg aaattgctga
    24361 ccgcactctg cgtgctggtg ggcctgcgct gttgttcgaa aaccctaaag gctactcaat
    24421 gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg gcgatgggca tggggcagga
    24481 agatgtttcg gcgctgcgtg aagttggtaa attattggcg tttctgaaag agccggagcc
    24541 gccaaaaggt ttccgcgacc tgtttgataa actgccgcag tttaagcagg tgttaaacat
    24601 gccgacaaag cgactgcgtg gtgcgccctg ccaacaaaaa atcgtctctg gcgatgacgt
    24661 cgatctcaat cgcattccca ttatgacctg ctggccggaa gatgccgcgc cgctgattac
    24721 ctgggggctg accgtaacgc gcggcccgca taaagagcgg cagaatctgg gcatttatcg
    24781 ccagcagctg attggtaaaa acaaactgat tatgcgctgg ctgtcgcatc gcggcggcgc
    24841 gctggattat caggagtggt gtgcggcgca tccgggcgaa cgtttcccgg tttctgtggc
    24901 gctgggtgcc gatcctgcca cgattctcgg tgcagtcacc cccgttccgg atacgctttc
    24961 agagtatgcg tttgccggat tgctacgcgg caccaaaacc gaagtagtaa agtgtatttc
    25021 caatgacctc gaagtgccag ccagtgccga aatcgtcctg gaagggtata tcgatcctgg
    25081 tgagatggcg ccggaagggc cgtatggtga ccacacaggt tactataatg aagtcgatag
    25141 tttcccggtg tttaccgtga cgcatattac ccagcgtgaa gatgcgattt accattccac
    25201 ctataccggg cgtccgccag atgagcccgc ggtactgggc gtggcgttga acgaagtgtt
    25261 tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat ttttacctgc cgccggaagg
    25321 ctgttcttat cgtctggcgg tagtgacgat caaaaaacag tacgccggac acgcgaagcg
    25381 cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg tacactaaat ttgtgatcgt
    25441 ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg atttgggcga ttaccacccg
    25501 tatggacccg gcgcgggata ctgttctggt agaaaatacg cctattgatt atctggattt
    25561 tgcctcgcct gtctccgggc tgggttcaaa aatggggctg gatgccacga ataaatggcc
    25621 gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa gatccagatg ttgtcgcgca
    25681 tattgacgcc atctgggatg aactggctat ttttaacaac ggtaaaagcg cctgatgcgc
    25741 gtttgttttg ccctatttat cgatccgaca gagaaagcgc atgacaacct taagctgtaa
    25801 agtgacctcg gtagaagcta tcacggatac cgtatatcgt gtccgcatcg tgccagacgc
    25861 ggccttttct tttcgtgctg gtcagtattt gatggtagtg atggatgagc gcgacaaacg
    25921 tccgttctca atggcctcga cgccggatga aaaagggttt atcgagctgc atattggcgc
    25981 ttctgaaatc aacctttacg cgaaagcagt catggaccgc atcctcaaag atcatcaaat
    26041 cgtggtcgac attccccacg gagaagcgtg gctgcgcgat gatgaagagc gtccgatgat
    26101 tttgattgcg ggcggcaccg ggttctctta tgcccgctcg attttgctga cagcgttggc
    26161 gcgtaaccca aaccgtgata tcaccattta ctggggcggg cgtgaagagc agcatctgta
    26221 tgatctctgc gagcttgagg cgctttcgtt aaagcaccct ggtctgcaag tggtgccggt
    26281 ggttgaacaa ccggaagcag actggcgtgg gcgtactggt accgtgttaa cggcggtatt
    26341 gcaggatcat ggtacgctgg cagagcatga tatctatatt gccggacgtt ttgagatggc
    26401 gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg cgggaagatc gcctgtttgg
    26461 cgatgcgttt gcatttatct gagatataaa aaaacccgcc cctgacaggc gggaagaacg
    26521 gcaactaaaa ctttattcag tggcatttag atctatgtcg catccggcaa ttggtgcacg
    26581 atgcctgatg cgacgctggc gcgtcttatc aggcctacat tggtgccgga tcggtagacc
    26641 ggataaggcg ttcacgccgc atccggcaag tggttaaacc cgctcaaaca ccgtcgcaat
    26701 accctgaccc ataccgatac acatcgtcgc cagaccaaac tgaacgtctt tgcgttccat
    26761 cagattcagc agcgtggtgc tgatacgcgc accggaacaa cccagcggat gacccagcgc
    26821 gatcgcacca ccattgaggt tgatcttctc gtcaatctgc tccattagcc cgagatcttt
    26881 aatgcacggc aggatctgcg cggcaaaggc ttcgttcatc tcaaacacgc cgatatcgct
    26941 ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc ggaaccgggc cgtaacccat
    27001 aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca cgagcgcgcg gcttaagacc
    27061 taattcatgg gcgcggcttt cactcatcac cagcatggca gctgcgccat cggaaagtgc
    27121 agaagatgtg cccgccgtta ccgtaccgtt tactggatca aacgccggac gcagcgtggc
    27181 gagggcttcc acggtggttt ccgggcgaat cacttcgtcg taattaaact gcttcaggac
    27241 gccgtcggca tcgtgaccac cagttgggat gatttcattt ttaaatgcgg ccgactgcgt
    27301 ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca tcctgcattt cacggctgat
    27361 accgtgcata cgcgccagca tttctgccgt taagcccatc atgcccgccg ctttggcgac
    27421 attgcggctc aggccggggt gaaaatcgac gccgtgactc atcggcacat ggcccatatg
    27481 ctccacgccg ccaaccagac atgcctgcgc atcgccagtc atgatcattc gtgctgcgtc
    27541 atgcagtgcc tgcatggatg aaccacacaa gcgattgacg gtaaccgccg ggacagagtg
    27601 cggcacttct gccagcagcg ccgcgttacg ggcgatgtta aaaccctgct ccagcgtctg
    27661 ttgcacacaa ctccagtaaa tatcgtcgag ggccgccgct tccagcgccg ggttacgcgc
    27721 cagcaggcta cgcattaaat gagcggagag atcttccgca cgcacgttac gaaaagcacc
    27781 gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca atgacaacct gttccattgt
    27841 gactccttaa gccgttttca ggtcgccaac cggacgggct ggctcaaccg gaggatagta
    27901 cggttcgtta tgacgcgctt tattacgcag accttccggc acttcataca gcgggccgag
    27961 gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta ccgagggtgt ccagccagcg
    28021 gaacgcgccg ccgtggaacg gagggaagcc caggccgtag accagcgcca tatccgcttc
    28081 cgccggagtg gcgataatgc cctcttccag acagcgcacc acttcgttga ccatcgggat
    28141 catcatgcgg gcgataatct cttcttcgct gaaatcgcgc ttcggttggc tcacttctgc
    28201 cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc ttacctttgc tgtcttcttt
    28261 ataacgccag aaaccgaggc cgttcttctg accaaagcgg ttggcatcaa acagcgcgtc
    28321 gatggcatcg cggtaatctt tctgcatccg ctgcgggaag cctgctgcca tgacagcctg
    28381 agcgtgatgc gcggtatcaa tgcccacaac gtccagcaga tatgccgggc ccatcggcca
    28441 gccaaactgt ttttccatca ctttgtcgat cttgcggaaa tccgcgccgt cgcgcagcag
    28501 ctggctgaaa ccggcgaaat acgggaacag cacgcggtta acaaagaagc cggggcagtc
    28561 gttaaccaca atcggcgtct tgcccatctt gctcgcccag gcgacaactt tcgcgatggt
    28621 ttcgtcggag cttttctcgc cgcgaataat ttctaccaac ggcattcggt ggaccgggtt
    28681 aaagaagtgc atcccgcaga agttttccgg gcgttccagc gcgttggcca gttcgctgat
    28741 aggaatggtt gaagtgttag acgccagcac ggtatcctgg cgtacttttt gttcggtttc
    28801 tgccagtacg gcttttttca ctttcgggtt ttcaacaacc gcttctacca caatatccac
    28861 gcggtcaaat ccggcgtagt cgagcgttgg gtggatcgtg gagatcacgc cagccagttt
    28921 cagaccatcg atcttgccgc gctcaagctg cttgttcagc agtttcgcgg cttcggtcat
    28981 gccgagggtt aacgacttgt cgttgatatc tttcatgaca accggcacgc ctttccacgc
    29041 ggactggtaa gcgataccgc cacccatgat gcctgcgccc agcacagcag cctgtttcgg
    29101 ggtttcaacg tctttggtga gtttcttcgc tttgcctttt acatattgat cgttaaggaa
    29161 aatgccgacc agtgcgcgag cttcgttggt atgcgccagc gggacaaaac ttttgttttc
    29221 cagatttaag gcttcttcac gaccaaaacg ggccgcagct tcaatggttt ttactgcggt
    29281 gatgggggcc ggataatgtt tccccgccgt ttgcgcgacc attcctttag cgatggtgaa
    29341 gctcatggtg gcttcaatct tgctcagttt tagcggttcc agcttcggct gacgtttcgc
    29401 tttccagtcg aggtcgccgt taatggcctg gcgtaaaatc gccatcgccc cttcaatcag
    29461 tttttctgct ttgactacgc catccaccag accgattttc agcgcctgat ccgcgccgac
    29521 atctttaccg gcggcaatga tttccagtgc actgtcagcg cccagcatac gtggcatacg
    29581 tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt tccggcagac cgatgcgcag
    29641 atccggcgtc gccagacgat aatcggtcgc cagcacgcat tcgcagccac cacccagcgc
    29701 atagccgttg acggcagcaa tggtcggcac cggcagatct tccaggcgat taaacacgct
    29761 attggcaaag tgcagccact gacttaactg ttcttcagga acgaggaaca gggacaaaaa
    29821 ttcggtgata tcagcaccga cgataaaggc tgctttgttc gaacgcagca gcagcccttt
    29881 tagatctgat tgctgttcca gcacgccgat ggcctcgccg aggctggcga cggtcgcagt
    29941 gtcgagttta ttaactgaac ctggggcatc aaataccagt tcggcaatgc catcttccag
    30001 ccagtcaagg tacagggtgt cgcctttgta aagcatgtca gtctcctgaa tccgcaaggt
    30061 gatctggtcg taccagatgc atcggagtgt gtgttttgtg ttaaaaatat gcaaataaaa
    30121 gattaaagaa atgccgatct gatcacgctc ggcagaaatc acgctctgga tgaacgatgt
    30181 gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt aaaaaatgga atcactggcc
    30241 tcgctctata aaaatcatat agctacctta caagaacgga ctcgcgatgc gctggcgcgc
    30301 ttcaagctgg atgcattact tattcactcc ggcgagctgt tcaacgtttt tctcgacgat
    30361 catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt gggtgccggt aactcaggtg
    30421 ccaaactgct ggttgctggt ggatggcgtg aacaagccga aactgtggtt ctatctgccg
    30481 gttgattact ggcacaacgt cgaaccgctg ccgacctcct tctggactga agatgtggaa
    30541 gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc tgcctgctgc gcgcggcaat
    30601 atcggttata tcggtccggt gccggaacgt gcgctgcaac tgggtattga ggccagcaac
    30661 atcaacccga aaggggtgat cgactacctg cattactacc gctccttcaa aaccgagtac
    30721 gaactggcct gtatgcgtga agcgcagaaa atggcggtca atggtcatcg tgcggcagaa
    30781 gaagcgttcc gttctggcat gagcgagttc gatatcaata ttgcctatct gaccgcgacc
    30841 ggtcatcgtg ataccgacgt accttacagc aacattgtgg cgcttaacga acacgctgcg
    30901 gtgttgcatt acaccaaact ggaccaccag gcaccggaag agatgcgcag cttcctgctg
    30961 gatgccgggg ccgaatataa cggctatgcg gctgacctga cccgtacctg gtcggcaaaa
    31021 agcgacaacg actacgcaca gctggtgaaa gacgtaaatg atgaacaact ggcgctgatc
    31081 gccaccatga aagcaggcgt cagctatgtg gattaccaca tccagttcca tcagcgcatc
    31141 gccaaactgc tgcgtaaaca tcaaatcatc accgatatga gtgaagaagc gatggtcgaa
    31201 aacgatctca ccggaccgtt tatgccgcac ggtattggcc atccgctggg cctgcaggtg
    31261 catgacgtcg ccggttttat gcaggatgat agcggtacac acctcgcggc accggcaaaa
    31321 tatccgtacc tgcgctgcac ccgtattctc cagccgggca tggtgttaac catcgaaccg
    31381 ggtatctact tcattgaatc gcttctggca ccgtggcgtg aagggcagtt cagcaagcac
    31441 ttcaactggc agaaaattga agcactgaaa ccgttcggcg gcattcgtat cgaagacaac
    31501 gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg atctgaaact ggcgtgatgg
    31561 aaagctggtt aattcctgcg gcaccggtca cggtcgttga agagatcaaa aagagccgct
    31621 tcataacgat gttggcgcat accgatggcg ttgaggcggc gaaagcgttt gttgaatcgg
    31681 tgcgggcaga acaccccgat gcccgccacc attgcgtggc gtgggtggcg ggtgcgccgg
    31741 atgattctca acagctgggt ttctctgacg acggggagcc ggcgggaacg gcaggtaaac
    31801 cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat taccgctgtg gtagtgcgct
    31861 actacggcgg catattgctt ggcaccggtg ggttagtgaa agcgtatggc ggcggcgtga
    31921 atcaggcgct gcgccagcta acgacccaac gcaagacgcc attaaccgaa tatactttgc
    31981 aatgtgaata tagtcagtta accggcattg aagcgttgct ggggcagtgt gacggcaaaa
    32041 ttatcaacag tgattatcag gcattcgttc tgctgcgggt ggcgcttccg gcggcgaaag
    32101 tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg ttcattgcaa ttgttagcga
    32161 ttgaagaata atccccactt cgttttgcag aactaaggaa gcggcagaga tgcattttcg
    32221 cgccattacc cgaatcgttg gactactggt catcttattt tcagggacca tgattatccc
    32281 tgggctggta gcactcatct accgggatgg agcgggccgc gcttttaccc agaccttttt
    32341 tgtcgccctc gccattggct ctatgctgtg gtggccgaac cgcaaagaga agggcgaact
    32401 gaaatcccgt gaggggtttc tgatagtggt gctgttttgg accgtgctgg gtagtgtcgg
    32461 tgcgctccct tttatcttct cggaaagccc gaacctcacg attaccgatg cgttttttga
    32521 atctttctct ggcctgacca ccaccggggc cactacgctg gtggggctgg attcgctccc
    32581 tcatgccatc ctcttttatc gccagatgct gcaatggttt ggcgggatgg ggatcatcgt
    32641 gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg atgcagctct atcgcgcaga
    32701 aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga attgcggaaa cggcgaaaac
    32761 cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg ctggcgttgt ggtttgccgg
    32821 aatggatgcc attgatgcca tcggccatag ctttgcgact atcgctattg gcggcttctc
    32881 gacacatgat gccagtatcg gttatttcga cagtccgact attaacacta tcattgctat
    32941 cttcctgctg atctccggct gtaactacgg tctgcacttt tcactgttaa gtgggcgtag
    33001 tctgaaggtt tattggcgcg atccggaatt tcgcatgttt atcggcgtac agtttacgct
    33061 ggtggttatt tgtacactcg tactgtggtt tcataatgtc tacagttcgg cgctgatgac
    33121 aattaaccag gcgtttttcc aggtggtatc gatggcgaca accgccgggt ttacgactga
    33181 cagcattgcc cgctggccgc tctttttgcc ggtactgctt ttatgttcag cttttatcgg
    33241 cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc cgcatcctgc tgctgtttaa
    33301 gcaggggaac cgtgagctga aacgactggt gcatccgaac gccgtctata gcattaagct
    33361 ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt tggggatttt tctccgccta
    33421 tgcattggtg tttattgtca gtatgctggc gattatcgcc acgggcgtgg atgatttttc
    33481 tgcctttgcc tcagttgttg cgacattgaa taacctgggg ccagggcttg gcgtggttgc
    33541 tgataacttt accagtatga acccggtggc taaatggatc ctgattgcca acatgctgtt
    33601 tggtcgtctc gaggtcttta cattgctggt gctctttacc ccgactttct ggcgtgaatg
    33661 atggagtaat acgtgaaaac attaattctt ttctcaacaa gggacggaca aacgcgcgag
    33721 attgcctcct acctggcttc ggaactgaaa gaactgggga tccagacgga tgtcgccaat
    33781 gtgcaccgca ttgaagaacc acaatgggaa aactatgacc gtgtggtcat tggtgcttct
    33841 attcgctatg gtcactacca ctctgcgttc caggaatttg tcaaaaaaca tgcgacgcga
    33901 ctgaattcga tgccgagcgc cttttattcc gtaaacctgg tggcgcgcaa accggagaag
    33961 cgtactccac agaccaacag ctacgcgcgg aagtttctga tgaactcgca atggcgtccc
    34021 gatcgctgcg cggtcattgc cggggcgctg cgttacccac gttatcgctg gtacgaccgt
    34081 tttatgctca agctgattat gaagatgtca ggcggtgaaa cggatacgcg caaagaagtt
    34141 gtctataccg attgggagca ggtggcgaat ttcgcccgag aaatcgccca tttaaccgac
    34201 aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc ggtcagaaaa ttattttaaa
    34261 tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca cggaacaacg
    34321 gcaaacacgc cgacgggtca gcggggttct cctgagaatt ccg
//
LOCUS       DADLSB010000026        33852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.026, whole genome shotgun sequence.
ACCESSION   DADLSB010000026 DADLSB010000000
VERSION     DADLSB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.026"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(17..868)
                     /gene="pdxK"
                     /locus_tag="JN331_001241"
                     /gene_synonym="thiJ"
     CDS             complement(17..868)
                     /gene="pdxK"
                     /locus_tag="JN331_001241"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY7259434.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHEIDELI
                     LPPLAEA"
     gene            973..1347
                     /locus_tag="JN331_001242"
     CDS             973..1347
                     /locus_tag="JN331_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY7259435.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQMDKTVPAEGN"
     gene            1380..2103
                     /locus_tag="JN331_001243"
                     /pseudo
     CDS             1380..2103
                     /locus_tag="JN331_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(2307..3218)
                     /gene="cysM"
                     /locus_tag="JN331_001244"
     CDS             complement(2307..3218)
                     /gene="cysM"
                     /locus_tag="JN331_001244"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY7259436.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMLELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(3352..4449)
                     /gene="cysA"
                     /locus_tag="JN331_001245"
     CDS             complement(3352..4449)
                     /gene="cysA"
                     /locus_tag="JN331_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY7259437.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(4439..5314)
                     /gene="cysW"
                     /locus_tag="JN331_001246"
     CDS             complement(4439..5314)
                     /gene="cysW"
                     /locus_tag="JN331_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY7259438.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(5314..6147)
                     /gene="cysT"
                     /locus_tag="JN331_001247"
     CDS             complement(5314..6147)
                     /gene="cysT"
                     /locus_tag="JN331_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY7259439.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYIWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGLEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(6147..7163)
                     /locus_tag="JN331_001248"
     CDS             complement(6147..7163)
                     /locus_tag="JN331_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7259440.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(7467..8258)
                     /gene="ucpA"
                     /locus_tag="JN331_001249"
     CDS             complement(7467..8258)
                     /gene="ucpA"
                     /locus_tag="JN331_001249"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY7259441.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(8387..9244)
                     /gene="murR"
                     /locus_tag="JN331_001250"
     CDS             complement(8387..9244)
                     /gene="murR"
                     /locus_tag="JN331_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY7259442.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            9408..10304
                     /gene="murQ"
                     /locus_tag="JN331_001251"
     CDS             9408..10304
                     /gene="murQ"
                     /locus_tag="JN331_001251"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY7259443.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTELMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            10308..11732
                     /gene="murP"
                     /locus_tag="JN331_001252"
     CDS             10308..11732
                     /gene="murP"
                     /locus_tag="JN331_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY7259444.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(11912..12811)
                     /gene="yfeX"
                     /locus_tag="JN331_001253"
     CDS             complement(11912..12811)
                     /gene="yfeX"
                     /locus_tag="JN331_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY7259445.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVTVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12907..13482)
                     /locus_tag="JN331_001254"
     CDS             complement(12907..13482)
                     /locus_tag="JN331_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY7259446.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13543..13992)
                     /locus_tag="JN331_001255"
     CDS             complement(13543..13992)
                     /locus_tag="JN331_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY7259447.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(13979..14404)
                     /locus_tag="JN331_001256"
     CDS             complement(13979..14404)
                     /locus_tag="JN331_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY7259448.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14618..15487
                     /gene="amiA"
                     /locus_tag="JN331_001257"
     CDS             14618..15487
                     /gene="amiA"
                     /locus_tag="JN331_001257"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY7259449.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVINYF
                     HWFDNQKAHSKKR"
     gene            15491..16390
                     /gene="hemF"
                     /locus_tag="JN331_001258"
     CDS             15491..16390
                     /gene="hemF"
                     /locus_tag="JN331_001258"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY7259450.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16396..17448)
                     /gene="eutR"
                     /locus_tag="JN331_001259"
     CDS             complement(16396..17448)
                     /gene="eutR"
                     /locus_tag="JN331_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAY7259451.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17494..17993)
                     /gene="eutK"
                     /locus_tag="JN331_001260"
                     /pseudo
     CDS             complement(17494..17993)
                     /gene="eutK"
                     /locus_tag="JN331_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
     gene            complement(18006..18665)
                     /gene="eutL"
                     /locus_tag="JN331_001261"
     CDS             complement(18006..18665)
                     /gene="eutL"
                     /locus_tag="JN331_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY7259452.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPQHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18675..19562)
                     /locus_tag="JN331_001262"
     CDS             complement(18675..19562)
                     /locus_tag="JN331_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY7259453.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19583..20944)
                     /locus_tag="JN331_001263"
     CDS             complement(19583..20944)
                     /locus_tag="JN331_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAY7259454.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSSVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            21132..22327
                     /locus_tag="JN331_001264"
                     /pseudo
     CDS             21132..22327
                     /locus_tag="JN331_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416937.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            22573..23160
                     /locus_tag="JN331_001265"
     CDS             22573..23160
                     /locus_tag="JN331_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259455.1"
                     /translation="MNEIKKPGKAATSSGRYEKQAKGEFLMSDMLAVEQETNNDVRQY
                     LNKINELRNKAPKNEETKHEEHTPDHYEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLY
                     ERWEKEHTVNDEAVLRALCYFAGTGKNSQLGWCRVGRGTIDKRARLSKNTVKKCLDRL
                     VHHFKLVERTEGYIPGSAERECNEYQLLFKPYNMK"
     gene            24610..24801
                     /locus_tag="JN331_001266"
     CDS             24610..24801
                     /locus_tag="JN331_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7259456.1"
                     /translation="MNDSELMTVNEVAALFGVTRRTIFRWMNKIKGWPVPVSPIDSRI
                     KFIRSEILEFYKNKGARHQ"
     gene            24941..25204
                     /locus_tag="JN331_001267"
     CDS             24941..25204
                     /locus_tag="JN331_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259457.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFQEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKARLLFELSRRFGVSISTFK"
     gene            25222..25764
                     /locus_tag="JN331_001268"
     CDS             25222..25764
                     /locus_tag="JN331_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259458.1"
                     /translation="MDAFDVLTNKGDELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIEIIKPQIPQIVADAIKAAGLEKRIAGLKTNVEKMEMHKQSGAPTPDEYFSPD
                     AEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     AADPEAEQRKRVFNAIGIRY"
     gene            25861..26190
                     /locus_tag="JN331_001269"
     CDS             25861..26190
                     /locus_tag="JN331_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259459.1"
                     /translation="MFGNYISTSPEKIIMLALRIMQGIAKTLAEHVLDLKHSPLSKQA
                     MKRQTLRLWAEYSLGTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGID
                     INDGTGD"
     gene            26229..26435
                     /locus_tag="JN331_001270"
     CDS             26229..26435
                     /locus_tag="JN331_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259460.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            26894..27232
                     /locus_tag="JN331_001271"
     CDS             26894..27232
                     /locus_tag="JN331_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7259461.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            27213..27491
                     /locus_tag="JN331_001272"
     CDS             27213..27491
                     /locus_tag="JN331_001272"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259462.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDEKTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            27484..28944
                     /locus_tag="JN331_001273"
     CDS             27484..28944
                     /locus_tag="JN331_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7259463.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            28966..29787
                     /gene="murI"
                     /locus_tag="JN331_001274"
     CDS             28966..29787
                     /gene="murI"
                     /locus_tag="JN331_001274"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7259464.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(29906..30600)
                     /locus_tag="JN331_001275"
     CDS             complement(join(29906..30354,30354..30600))
                     /locus_tag="JN331_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7259465.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(31124..32527)
                     /gene="eutA"
                     /locus_tag="JN331_001276"
     CDS             complement(31124..32527)
                     /gene="eutA"
                     /locus_tag="JN331_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY7259466.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGVAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(32524..33750)
                     /gene="eutH"
                     /locus_tag="JN331_001277"
     CDS             complement(32524..33750)
                     /gene="eutH"
                     /locus_tag="JN331_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAY7259467.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMR
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
BASE COUNT         8561 a         8828 c         8299 g         8164 t
ORIGIN      
        1 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgatctcat
       61 gctgctgggt gtagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
      121 ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
      181 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
      241 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
      301 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
      361 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccctgc gccagcggca
      421 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
      481 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
      541 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
      601 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt
      661 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
      721 gcacggcaaa gacattcaag ccgttctgtt ttatagcagg caccgcaatg ctgttgccta
      781 cgcttccgta cactacctgc gactgcacgg cgacgatatc cgcctgcagc gccctactct
      841 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
      901 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctattac aaaacaagga
      961 taaggtaatt cagtgaagaa aatcatttgt ctggccatta cgctgctaat gacactcccc
     1021 gcttacgcga agttaactgt tcatgaagaa gcccgcatca acgccatgct ggagggatta
     1081 gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg cgatgaagcg
     1141 gtttcccacc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
     1201 tttattgata aggtagcttc gtcgtcatcg attaccggga agccgtatat cgtgaagatc
     1261 cccggtaaga gcgatgagaa cgcacagcct tttttacatg cgttaattgc gcagatggat
     1321 aaaacggtgc ctgcggaagg aaattaatcc gctttggtaa ggcatttaca ggaggtaaca
     1381 tgaaaaaatg ctttatttgt cacgatgaaa aatcgaataa attttggtgg atagattacg
     1441 aaggggatag tttagctgtc aactatggca aggtaggtag tattggtaaa ttccatacaa
     1501 aagaattcga taatgaagaa cagtgtctga aagaagccag taaattgatt gccgcaaaaa
     1561 tgaaaaaagg atatcaagaa actccagact ttaacttcat ggatctctac tattttgatg
     1621 atgaagagat tgggttacat cttaaaacgt cacacccaaa cttccagtgc cattttactg
     1681 atccacttta tatgtgttgc tgggatgaag aatctccttt tggtagcgat gaaggtgctg
     1741 atgctctaaa cgaacttgaa aatagcctcc gttctggact gtgctgattt ccctcaaatg
     1801 ttaattgaaa ctatgtgggg tatgaaatac atcgctatgg acagtattct tgaagaggat
     1861 gtgcgtgcgc aattactagc cgatgaaatg agctctatcc agagcaatat gattacctac
     1921 gcgactgcat tcggtcagat caaagtcatg ggtaaaatct cccataaact taaaaagatg
     1981 ggactcaatg cactagcgcg tcatcagctt accgcaaaaa ttcttcaatg gggtgacggt
     2041 caggactcac caatacttca aaaaatgatt gatgacctta ctgcttttcc acacgaaaat
     2101 taaaaactgc atttgtcggc agcaacaact gccgacaaat cagattattg acaaagtgca
     2161 ctttgtttat gccggatgcg gcgtaaacgc ctgatccggc ataaaaatat tgctaattca
     2221 atatattgca gaaacattgt aggcctgata agcggagcgc atcaggcagt tttgcgtttg
     2281 tcatcagtct ccaatgctat taatccttaa atccctgccc cctgactaaa atgctcttcc
     2341 ccaaacactc cggtggagag gtagcgatcg ccacgatcgc agatgatcgc taccaccacc
     2401 gcaccagggt tagcttttgc cacccgcagt gctccagcaa ccgcgccgcc tgagctgacg
     2461 ccacagaata ttccttcccg caccgccagt tcgagcatgg tgttttccgc atcgcgctga
     2521 tgaatatcca gcacctcatc caccagcgaa gcgttgaaaa tccccggcag atattccgca
     2581 ggccagcggc gaatgcccgg aatactgctg ccttcttccg gttgcaggcc aacaatggtc
     2641 accggtttgg attgttcgcg cataaagcgt gaaacgccgg tgatagtgcc ggtcgtcccc
     2701 atgctggaga caaaatgagt gatgcgcccg ccggtttgct gccagatttc cggcccggtg
     2761 gtggtgtaat gcgcataagg gttatcggga ttattgaact gatcgagcag ctttccttcg
     2821 ccacgattcg ccatctccag cgccagatcg cgcgcacctt ccatgccctg ctctttggtg
     2881 acaagaatca gttccgcacc ataagcacgc atcgccgcac ggcgttcctg gctcatgttg
     2941 tcgggcatca gcaatttcat gcgatagcct ttcagcgcgg caatcattgc cagcgcaatg
     3001 ccggtgttac cactggtggc ttcgattaag acatcacccg gtttaatttc cccgcgcttt
     3061 tccgcctcga cgatcatcga aagtgccgca cgatctttca ccgaacctgc cgggttattg
     3121 ccttccagtt ttaaccacac ttcactgccg ttatccggcc ccattcgctg caacttcacc
     3181 agaggcgtat tgcctattgt ttgttctaat gtactcacga tctctatcca tacgtggtgt
     3241 tgcctgatgc gacgcttgcg cgtcttatca ggcctacagg ttacaaacct tgccattaaa
     3301 aagcccggat cgcgggaagc gcctccgggc gtttaacatt cactcaacct atcaggcgct
     3361 ttgtgcgaga gcaagttcct catcgcgggt ttcaatacgc tcgtcgccgt tatacagccg
     3421 cgcatgttgc agaccaacga ataaacgctc gccacgctgc ggggcatcgt cgccatgcat
     3481 cacgaccgtc agcggttcgt tgtaccaccc cagcggctgc accactaatt gggtgtagtg
     3541 acctttcggg ctggcttcca gtacctgtac cggcagcggc gaatcgagac tggtacggcg
     3601 gctgatatcc acttcccacg ggcgcaggaa gagatccacc ggaccctgat acgcaggtgt
     3661 gtagcccagc ggccagcgat gcgcgccaac atggaactgc ccgccgcgaa tggttccctg
     3721 caggcggttc acttcgccca taaattcgag tacaaaacgg gtcgctggtt cgcgccatac
     3781 ctgattcggc gcatcagcct gttcaatatt cccctgactc atcaccacta cacgatcggc
     3841 tacttcggtc gcctcttcct ggtcgtgggt cacaaacacg ctggtgaatt tcagctcttc
     3901 atgcagttga cgcagccagc gacgcagctc tttacgcacc tgcgcatcca gcgcgccaaa
     3961 cggttcatca agcagcagaa tttgcggttc tacagccagc gcgcgcgcca gcgccacacg
     4021 ctgtttctgg ccgccggaaa gctgcgccgg ataacgatcc gccagatgcg caagctggac
     4081 catttccagc aattttgtca ctttcgcttt gatggctgcg gcattcgggc gctcgcgacg
     4141 cggcagcacc gtcaggccaa aagtgatatt gtcgaatacc gtcatatggc ggaacagcgc
     4201 gtaatgctgg aacacgaaac cgactttacg atcgcgcgcg tgcaggcgac tcacgtcggt
     4261 gccgtggaag cgaatatgcc cgctggtttg atgctccagc ccggcgataa tccgcagcag
     4321 cgtggttttc ccggaaccgg acggccccag caacgcgacc atctgaccag aaggaatatc
     4381 cagtgagata tcgttcagca cctgggtgcg accaaaagac ttcttgatat tggcaatctc
     4441 aatgctcatg atgctcctcc tgctgcgcgc gtttttcctg attctccagg cgccactgca
     4501 acatactctt taaaaacagg gtgataatcg ccatcagcgt caacagcgcg gcggcggtaa
     4561 aggagccgac ggtgttgtag tcctgctcca acaattcaat ctgtaacggc agcgaaagtg
     4621 tttcgccgcg aatcgagccg gaaaccaccg acactgcacc aaactcgcca atcgcgcggg
     4681 cgttggtcag caccacgccg taaagcagcg cccagcgaat attcggcaat gtgacgcgac
     4741 ggaacatctg ccagccggac gcgccaagca aaatcgccgc ttcgtcttcc tggctgccct
     4801 ggcttaacat caccggcacc agctcgcgca ccacaaacgg acacgtcaca aagatggtga
     4861 ccagcaccat tccgggccag gagaacataa tttgcagatt atgttcatcc agccagccgc
     4921 cgagcgggcc gttggagccg tagaacagca gatacaccag accggcaacc accggcgata
     4981 cggcaaacgg aatgtccagt agcgtcagca gtaactggcg tcccgggaag ttaaagcgcg
     5041 tcaccagcca ggccagcaga atgccgaaca ccaggtttac cggtacggca atcagcgcga
     5101 tcatcaccgt cagccagatg gcgtgcagca tgtccggatc ggccagattc tgtaaaaccg
     5161 gcatcagccc cttgctgaat gcctgcacga agatgtaaat catcggcacc agcaggatga
     5221 acgccgaaac cagcatcccg atgccaatca gaaaccattt gccccagtta atcgggcgcg
     5281 cgtcataacg cttcaattgg gtaacttccg ccattaatga cctaccacac gccgaccaaa
     5341 gcgactttgc agagtgttaa ttgagaacag cagcagcaga gatgccgcga ggatcaccga
     5401 agcaatcgcg ctcgctgccg ggtaatcaaa ctcctgtaag cgcacaaaaa tcatcagcga
     5461 cgtcacttcc gtcttccacg cgatatttcc ggcgataaaa atcaccgcgc caaattcacc
     5521 aagactacgg gtaaacgaca gcgccacgcc cgccaccagc gccggagaaa gctccggcag
     5581 caccactttg cagaaactct gccagcgcgt tgcaccaagc gtttccgccg cttcttcata
     5641 ttccaggcct aactcttcca gcaccggctg cacggtacgc accacaaacg gaatgctggt
     5701 aaaggccatc gccaccgcaa taccaagcca gatataggtg actttgatat caaacttcgc
     5761 cagccattca ccgtaaaaac cgtttacgga aaagagcgag gccagcgtta aaccggcgac
     5821 tgccgttggc agcgcaaagg gtaaatccat cagcgcatca agcagcgtgc ggcccggaaa
     5881 gcgataacgt gttaaaatcc acgccatcag cagaccgaaa acgccgttaa aaatcgatgc
     5941 cacaaacgcc gacagcagcg tcactttgta ggccgcgact acctgcgggt tggtgatcac
     6001 ctcccagtac tgcgcccagc tcatctcaga aagctgcatc accagcgcag agagcggcag
     6061 cagcaatatc aggcacacaa acagcagact ggtgccaagg cttaaagtaa agcccggcag
     6121 cacgcgtctt gaagaaactg caaacattac ttacgccccg ccgctaacag cttgtctaac
     6181 tcgccgccgc tggtgaagtg ggttttcatc acttccggcc atgagccaaa tttgtcttcc
     6241 acgcggaaca gttcggtctg cgggaattta tctttcagtt tgtccatgac ttccgggtta
     6301 ttcacacggt agtaatagtc ggtgatgatg gtctgcgcct gtgggctgta gagccagttc
     6361 aggtaggctt ttgcggcttt ttccgtaccg ttggcctgca catttttatc gacccacgcc
     6421 accgggaatt ccgccagaat attggttttc gggatcacca cttcaaagcc ctgtgcttca
     6481 tactgtttac ggatgttgtt cacttccgat tcaaagctaa tcagcacatc gcccaagcca
     6541 cgctcggcaa aagtggtggt tgcgccacga ccgccggtat cgaacacttc aacgtttttc
     6601 aggaactggg tcataaactg ttcggttttg gctttatcgc caccgtcagc tttatccgct
     6661 gcgccccatg ccgccagata ggtataacgc gcgttacccg acgttttcgg gttcgggaaa
     6721 atcagcttca cgtcggagcg caccaggtcg ttccagtcgt ggatattctt cgggttgccc
     6781 ttacgcacca ggaagcccat ggtggagtag aacggcgagc tattattcgg caggcgcgac
     6841 tgccagtcag ccgggatcag cttgcctttg tcgtgcagga tttgtacgtc ggtcacctgg
     6901 ttataagtga caacgtctgc ttttaagccc tgcaaaatcg ccagcgcctg ttttgatgac
     6961 ccggcatgag attgttttat cgtcagtttg tcgccgccgt tatcttttgc ccattgctgt
     7021 tcaaacggcg gattcagggc ggcaaacagc tcgcgggaga cgtcataaga actgttcagc
     7081 agttccgttg cctgtacatg gcccgccagc agcagagaag cgaccagcgc gagtgagttc
     7141 tttttcagta agttaacggc cattgcgcac ccttataaat ttaatgactt tctaatagcc
     7201 atcatattta taacggacgt gaaaggagta acggttttat ataccgtttg gtgatttgga
     7261 agttaaaaag ggaataagaa cttggttcaa caggcgtaat tgttgcaggc agtttggaca
     7321 cggacagcgc ggagaaaccg gagcgtacac atagtacgtg aggatttcga gcactgcccg
     7381 gggccaaaat gacaaatgaa atagcctggt gaacttagtt caagacgaaa tcctccccac
     7441 acatgcaggg aggaaacaga ggtgaatcag ataccgacgc taaccgtctc cggcagtgtg
     7501 ctgccgccat caatcacatt ctgtgtaccg gttaaatagc tggattcatc cgatgcgagg
     7561 aaggccgcca gttcgccgac ttccagcgga tcggcgaggc gacacagcgg gattgctttt
     7621 gccatttcag tcagcaccga ttctggatct tccgggttcg actggcgggc aatgctttcc
     7681 gccattggcg tgcggacgta tcccgggcag atggcgttaa cgcgaatacc ggattgcgcg
     7741 tactccaccg ccagcgattt agtcaggcca acaatcgccg ctttcgttaa ggcatacgcc
     7801 gtttcgccag gatcggccac catatcacca gtgactgaag acatcatcac aatgcgacca
     7861 tctttgcgcg caatcatctc cggcagcacc gccttcgtga cgttccatac gcctttaata
     7921 ttgatatcaa tatggaaatc gcgatcttca tcgctcatat cgaggaaact gcccagacga
     7981 caaacgcctg cgttattcac caggatatca atgcgccctt ctttttcctt cgcgcgtttg
     8041 atagctgcgg ctaccgacgc cgggtcacgc acatcggcga caaccgccgt acagcgatga
     8101 ccacgaccac acagttcgtc cgctagcttt tcgatctcag gggagatatc cagcaagatt
     8161 aggttcgcgc catgacgtgc aaaagttctg gcaattcctt cgccgattcc ctgcaatgcg
     8221 cccgtaatca gtgctgtctt gcccgtgagt ttacccattt taatgctctc cttgttaata
     8281 cagagcgtta cactccattc cctctgaaaa tacaggataa acaaaatatt gctgcggatc
     8341 agctcactaa ttcaggaaaa tctcaatcca gtcgctgctc tatgcatcat ttcaggcgtt
     8401 gtgttagctc actgctgcgc tgaatcattt tcaatgacgc cacatcattg agttgcacaa
     8461 gaccaacaaa cagtaaatcg gtaacagaat tttgcgccgt gcgggtagac atcgacgagc
     8521 tacgccattc ggtttcaccg gaaacggtat cgagcgtgaa atgcgccagc cgccgcagcg
     8581 gagagtcagc cagagaagta atggcaatca ccgttgcccc ctgctttcgt gccgcttctg
     8641 cgcacaagac aatctctttt ttactgccgc tgtaagagat agcgatttgt acatcgcctt
     8701 ttttcaacgc ttgggaaacg gtggcttgta cgtgcgtgtc ggcttcgcag gccacgcgat
     8761 agccaatttt catcagtttg aacgacagat cgcgccccac cagcgccgat ccgcccaggc
     8821 ccgttatctg gataaacggt gctttactga tgacctcaat gattttttgc agccgcgcgt
     8881 agtcgaacaa cgcacaggtt tgctccagcg ccagctcttt ttcgcgattc agtttgcggg
     8941 caatcacttc cagcgaatca tcactggtaa tcgaactgtg caggtgcagc gccgtggcat
     9001 tcgttttctc ccggctggca ctgtattcgc caattaacgc catacgcaat tcagtaaatc
     9061 cttgcgcgcc gagcttctgg gcaaatttca caatgctcga ctggctgata ccgagctgtt
     9121 tcgccatctg ccgtgaagag acggatttca gctcgctcac tctggcccgc agaaagtcag
     9181 caattttttg ctcgttcccg gtaaattcac tttccgcatt gcgtattttc gtcaggtaca
     9241 acatcttcca ctctcctggg ctggcagcaa aaattccgct taagttctta tgacgctctt
     9301 cacactctgc gagtgtagac tcaatgattc ctttaagtct gtcaattcgg gatatatgat
     9361 tccatacatc tgcaattatt tgatcgtaaa tagtaaggtc accaccgatg caactagaaa
     9421 agatgattac tgaaggctcg aacaccgcct cggctgaaat tgaccgcgta tcgacgctgg
     9481 aaatgtgccg gattatcaac gatgaagata aaaccgtacc gcttgccgtt gagcgcgtcc
     9541 tgccggatat cgccgcggcg atcgatgtta tccacgccca ggtcagcggc ggcggtcgtc
     9601 tgatttatct cggtgcggga acatccggtc gtctggggat tctggatgcc agcgaatgtc
     9661 cgcccaccta cggcgtgaaa ccgggtctgg tggttggttt gattgctgga ggcgaatatg
     9721 ccattcagca cgcggtggaa ggcgctgaag atagccggga aggtggtgtt aacgatctga
     9781 aaaatattaa tttaacggca caggatgtgg tggttggcat tgctgccagc ggtcgcacgc
     9841 cgtatgtgat tgccggactg gaatacgcac gccagctcgg ctgccgcaca gtgggaattt
     9901 cctgtaatcc ggggagcgcc gtttcaacca ccgctgagtt tgccattaca ccgattgtag
     9961 gtgccgaagt tgttaccggt tcttcgcgga tgaaagcagg tacagcgcag aaactggtgc
    10021 tcaatatgct ttccaccgag ctgatgatta aatccggcaa agtgttcggc aacctgatgg
    10081 tcgatgtggt cgccaccaac gaaaaactgc atgtgcgcca ggtcaatatc gttaaaaacg
    10141 ccaccggatg taatgccgaa caagcggaag cggcgttaat tgcctgcgag cgcaactgta
    10201 aaacggccat tgtgatggtg ctgaaaaatc tcgatgccgc agaagctaaa aaacgcctgg
    10261 atcaacacgg cggctttatt cgtcaggttt tagacaagga ataacccatg gccaaagaga
    10321 tcagcagtga acttctgaac accattctta cccgtgtcgg cggaccggga aatatcgcca
    10381 gttgtggtaa ctgtatgacg cgcctgcgtc tgggtgtaca tgacagttca ctggttgatc
    10441 ccaatatcaa aacgctggaa ggcgtgaagg gcgtcatttt gaccagcgac caggtgcagg
    10501 tcgtttttgg gcctggtaaa gcgcatcgtg ccgcaaaagc gatgagcgag ctgctgggcg
    10561 aggcaccggt acaggatgcc gcagaaatcg ccgcccagaa caaacgtcag ttaaaagcca
    10621 aacaaacctc cggcgtgcaa caatttctcg ccaaattcgc caccatcttc acgccgctga
    10681 tccccggttt tattgccgcc ggtctgctgc tggggatagc gacgttaatt gccacggtga
    10741 tgcacgttcc ggcagatgct cagggcacgc tacccgacgc gctgaatttt atgaaggtgt
    10801 tcagcaaagg tttgttcacc ttcctggtga ttctggtggg ctataacgcc gcccaggcat
    10861 ttggcggcac gggcgtcaat ggcgcaatta ttgccgcact ctttttgctc ggttataacc
    10921 ccgctgcaac caccggttac tacgccggtt ttcacgattt ctttggtctg cccatcgatc
    10981 cgcgcggcaa tattatcggc gtgctgattg ccgcgtgggc ctgcgcccgc attgaaggca
    11041 tggtgcgccg ctttatgccg gacgatctcg acatgctgct gacctcgtta atcaccctgc
    11101 tgatcaccgc tacgcttgcg tacctgatca ttatgccgct gggtggctgg ttattcgaag
    11161 gtatgtcatg gctgtttatg cacctgaaca gtaatccgtt cggttgtgcg gttttagccg
    11221 ggctgttcct gatcgccgtg gtatttggcg tgcatcaggg ctttattcct gtttacctcg
    11281 cgttaatgga cagccaggga ttcaacagct tatttcccat cctttcaatg gcaggcgcgg
    11341 gccaggtggg cgcggcgctg gcactctact ggcgggcgca accgcacagt gcgctacgca
    11401 gtcaggtacg cggggcgatt attcccggcc tgctgggcgt tggtgaaccg ctgatttacg
    11461 gtgtcaccct gccccgcatg aagccgtttg ttaccgcctg tttaggcggc gcggcgggcg
    11521 gtttgtttat cggattgata gcctggtggg gtctgccgat gggcttaaac agcgcctttg
    11581 ggccgtctgg tctggtggcg ctgccgctga tgacttccgc acaaggcatc cttccggcaa
    11641 tggcggttta tgctggcggg attctggtgg catgggtttg cgggtttatt ttcaccacgc
    11701 tctttggctg ccgtaacgtc aatctggact gatattatga aacggacaat gctctatctt
    11761 tctctgctgg ctgttagttg tagcgtcagc gccgcaaaat accctgttct gacagaaggt
    11821 agttgatcaa gtatatgcgg cgttaaagca gtaataaaaa ggccagtcag taatgttact
    11881 gcctggcctt taatcaatga atcagaaacg cttacagcgc catcaacttg tccagcgacg
    11941 gtgcgaaata atagccgccg gttaccggtt tggtgaaacg caacatcgca tcacgtttgc
    12001 catcggtatc gccaaacatg ctcagcagtt gctgctcaat gttatgcaga cgcgcgcagt
    12061 aggcgcagaa gtacagaccg tgagtgccac tggcggtgcc gtacggcagg ctctggcgaa
    12121 caatcttcag ccctttgcca tcttctttca gatcaacgcg ggtgaggtga gaggtttccg
    12181 gacgttcgtc gccgtcaatc tcttcgttgg cctctttggt gcgcccgatc atcatctcct
    12241 gatcgtgaac gctcatccgg ttgagctgct tcaggttgtg ctcccaacgc tgcacaaaca
    12301 catagctgcc gccggcatcc acgccgtctt tgataaccgt cacttcgcgg cgcgtctctt
    12361 cacccgccgg gttttctgta ccgtcaacaa agccgctcag gtcacgctct tccacccaac
    12421 ggaagccgtg gatctcttct ttcacttcaa tgcagtcacc aaaggcttcc atcgccgcct
    12481 gagcgacaga gaagtttacg tcgtgacgca gagaaagaat gtggatcaac acatcgaact
    12541 gggtcgtcgg cgcaaggcct ttaccgtagc ccggaaaatc tttcagctct tctgccccaa
    12601 cgccgccgct cagagcgcgc caggtgttgt taccaaaggc aaccaccgca ccaagatgcg
    12661 cgtccgggaa tttcgcttca aaagttgcca gtttgtcggc aaatgtttta ctggccgcac
    12721 gcagggcgtc aacttcccct ttcacgttgg cttcgatcca aatcgccgcg cggcaatgtt
    12781 ctggcaaaat gccactctga acctgagaca ttgttcctcc tgaaaataag aatgccacgc
    12841 aatccgtggc attgttgagc gctattttac gtgttttttc gcgacggcgg tttgttcaga
    12901 cgcaaattaa cgccgccaga taattttact gactttccag tttttcaggg tatcgtccga
    12961 aggcattaac ccttcaggac cacgccattc cccgctgaac tggtagctaa tatgttgact
    13021 gccttcggct ttacattcga cggcacgatt atcatcacca tcagcttttt ggcaattgcc
    13081 aaatgctttg ctgtaaaggt cgctaaacgg tgtaccgatt ttaacaccgg tgtcagcagg
    13141 aatatcgcta tccagcacgt caatgcgact gatcgtgcct tgatcgccgt taatcaccat
    13201 cgccacgttg tcgcctttca tcacttcaaa aaagcgcacc acgttgccgt tcgcggtttt
    13261 cattccgctg cgcaggcgat aatcgccatc aagcgcatca gcaatggctt gttcttgcag
    13321 tggtgtggac gccgttaatt cacccacgcc ctgctcgctc actttggtgg acgatccaaa
    13381 ccagttccac gggttagcgg cagaccagtt aactgacgac atcgtggaac agccggtcag
    13441 catcaacggc atagcgcata acattaaacg cagcgatttc atgtcacttc ctttggttat
    13501 tcaataacgt tgcttggagt gcaaattcac caaaaagtgc cgttattctt tttcttcatt
    13561 aaaacaagcg cgtaaacgtc gattggtcag cagccagatt aacgccacaa tatccgccac
    13621 taccagcgcc agaccgatac cgctaacgga ttcaccgttc agccacagcc acggttgcca
    13681 gcaaagtaaa accacctgcg ccaacagcaa cagaaaatag agcacatgcc aggtgcgagg
    13741 aaacgaagcc cgccgaccgc tcaacaaaaa cgccagcacc gccggaatac cgggaatcag
    13801 ccccagccag aaattatcgt gatcgggata aaacagattt agcagcgcag taccctgctc
    13861 gcgcgacgca ccggcaatga caaacagcac ccaggttcgc gcctgaagca atagcacaag
    13921 ccagaagagc aagggtaaac gcaggcgtcc gtgcgcatca taatggacag gatggaactc
    13981 agtactcttc atcttcaatc aaacgcttac ccagactcag cacgtcggcg tgttcatatc
    14041 ccaggcgttc atacattccg agcaccatgt cgttatcttc cggcacgttg atctgaattt
    14101 tcgggcagcc gcgagcaatc agctttttct ccagccggtt aagcaacgca ttggcaattc
    14161 cacgcccacg aaactctgga tgcacgccaa gataataagc agacccgcga tgcccgtcat
    14221 aaccgcccat caccgttccg accacttcac cgtttacctc agcgaccaaa aacaaactga
    14281 cgtcatggtt catcttacgc tcgatgtcca tttccggatc gttccacgga cgcagcaagt
    14341 cgcaacgctc ccaaagggtg atgacctctt cgaaatcttc ctggcgaaat acgcgtatct
    14401 ccatggtatt cgttaccttt ttgcgggtta aaaggctgat tatggcgtga acggtcgaat
    14461 tagccaatat ctgacgaaaa tcggttgaaa aagtggcata atggggagtt gtcaactatt
    14521 gaaatgaaaa gtaaaacaat tctcaacagc aaaccgtcgt aacggattac gcgatacgat
    14581 ataacatctg gaactttatt attacaactc aggccgtatg agcactttta aaccactaaa
    14641 aacactcact tcgcgccgcc aggtgctgaa agccggttta gctgccctga cgttgtcagg
    14701 aatgtcgcaa gccatcgcca aagacgaacc tttaaaaacc agcaacggac acagcaagcc
    14761 gaaagccaaa aaatctggcg gcaaacgtgt cgttgttctc gatccaggcc acggcggaat
    14821 tgataccgga gcgatcggac gcaacggttc gaaagaaaaa catgtggtgc tggcgattgc
    14881 taaaaacgtc cgttccattt tgcgtaatca tgggattgat gcgcgtttaa cgcgttctgg
    14941 cgatacgttt atcccacttt acgatcgcgt tgaaatcgcc cataaacatg gcgcagatct
    15001 gtttatgtca attcatgccg atggctttac caacccgaaa gctgccggtg cttcggtatt
    15061 tgccctctct aaccgtgggg caagtagcgc aatggcgaaa tacctgtctg aacgcgaaaa
    15121 ccgcgccgat gaagttgccg gtaaaaaggc gactgacaag gatcacctat tgcagcaagt
    15181 actgtttgat ctggtgcaaa ccgatactat taaaaacagc ctgacgcttg gctcgcatat
    15241 tctgaaaaag attaagccgg tgcataaact gcacagccgc aacaccgaac aagccgcgtt
    15301 tgtggtgttg aaatcgccgt cggttccttc ggtgctggtg gaaacctcgt ttatcaccaa
    15361 cccggaagaa gagcggctgt taggcacggc ggcgtttcgt cagaaaatcg ccacagcgat
    15421 tgctgaaggc gtgatcaatt atttccactg gttcgacaac cagaaagcac attcgaaaaa
    15481 gcgataagtt atgaaacccg acgcacacca ggttaaacag tttctgctca accttcagga
    15541 tacgatttgt cagcagctga ccgccgtcga tggcgcagaa tttgtcgaag atagttggca
    15601 gcgcgaagct ggcggcggcg ggcgcagtcg ggtgttgcgt aatggtggtg ttttcgaaca
    15661 ggcgggcgtc aacttttctc acgtccacgg tgaggcgatg cctgcttccg ccaccgctca
    15721 tcgcccggaa cttgccgggc gcagtttcga ggcgatgggc gtttcactgg tagtgcatcc
    15781 gcataacccg tatgttccca ccagccacgc gaatgtgcgt ttttttattg ccgaaaaacc
    15841 gggtgcagag cccgtttggt ggtttggcgg cggcttcgat ttaacccctt tctatggttt
    15901 tgaagaagac gccattcact ggcaccgcac cgcccgtgac ctgtgcctgc catttggtga
    15961 agacgtttat ccccgttaca aaaagtggtg cgacgattac ttctacctca aacatcgcaa
    16021 cgaacagcgc ggcattggcg ggctgttctt tgatgatctg aacacgccag atttcgacca
    16081 ctgttttgcc tttatgcagg cggtaggcaa aggctacacc gacgcttatt taccaattgt
    16141 agagcgacgt aaagcgatgg cctacggcga gcgcgagcgc aattttcagc tctaccgtcg
    16201 cggtcgttat gtcgagttca atctggtctg ggatcgcggc acgctgtttg gcctgcaaac
    16261 tggcgggcgc accgagtcga tcctgatgtc aatgccgcca ctggtacgct gggagtatga
    16321 ttatcagcca aaagatggca gcccagaagc ggcgttaagt gagtttatta aggtcaggga
    16381 ttgggtgtaa ctccctcacc cccattcccg catccgctga tgcaacgtca acgacggctt
    16441 ctcggcaaac agctgctggt aatccgtggc aaattgcccc agatgccaga atccccactg
    16501 catggcggcg tcttttaccg ttgtgctttg cgaccacggg cttatcagtt cgcggcgtac
    16561 ggcgttcagg cgaatgcgtt tcagccacgc gtttgggcca atgcctaaaa tagcgtgaaa
    16621 cgcgttttgt agcgtgcggc gactaacatg cagttgatta cacaaatcca gcaccgtcac
    16681 cggttcggac atgttttcca gcacatattc acgggcgcgg gaaagcaacc gacggtaact
    16741 ctgatgactg atgctttccg ccgtcaccat tggttgcgct tcttccagca tcgcccccat
    16801 cgccattagc aaattatccc ccagcacttt tcgcactgca ggctgatgga gattttccgg
    16861 gttctcgcaa aacgtcgcca gcgcctgttg gacaaagccc cacagcgcgg ctttatgctg
    16921 ctctttcact tccagcgccg actggctacg cagcatatgt aatacccgat ccggattgtg
    16981 caaaaagtta gcctgacggg tgatgacatc ttcagacagc actacgccca ggatcgtata
    17041 atcgtccggc gtacttaatt caaactcggt tccaccgggg cgcgtcgcta tttctgcgct
    17101 tcccagacat tgcgaaccga taaatccctg ctcaccgcgc gtcgccggaa tgccaaacca
    17161 gaacgagttc ggccagacca ggcacgactg gcgcagcgcc agaccggtgt attcacgaaa
    17221 aacctgaata tcatcgagta gaatttccgt aaattcacca tgaaacttgc ccggatgcag
    17281 ctgatcgtaa atctgctgcc aggcggtaat cgtcaaagca tgttcataga catccgttgt
    17341 ctgtcgttga tgaacattat ccacttcgac cttcggcgtg agcttcaggt tttcgggtaa
    17401 gggttcatga taaagatggt gcaaattggc tgtacgggtc tttttcatga tgttaatgcc
    17461 gggtgttgta ggacacccgg cacctccgac aggttaatgg ggcttgagac gataacgact
    17521 actgcgttta cgtaacgtcc cggcagaaaa gagctgttcg agcgcatttc tggcttttcc
    17581 agcggccagc caaagtgggc ggccacttct cctgccgtca ttccctgaca tactgatgtt
    17641 aacagcgcca acaactcgtc agcagattcc gcgacgataa ctggcgcgtc gggttccttt
    17701 acgggttgct tcggctggcg gttaaagcca gtaaccagcc actgggtgtc atcgtctggc
    17761 cgaccgatct ccttacggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg
    17821 tccagcgccg cacgacacgc cgtcagatcg ccttccacca ccagcgttaa gcgaccaggg
    17881 tcaagcactt cgtgactgag cagacgaacg ttagctgctt tgagcatggc atccgctgca
    17941 tctatcgcgg cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac
    18001 ctccgttacg cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaggcg
    18061 ttacaggctg ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt
    18121 tcagacggtg gcgggacgta ggtcaccagc tgtacgtcgg cagatttcaa cgcggcatca
    18181 atgccgtagg tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaagcgtg
    18241 atcccggcgg ttgatgaaag ataagaaccg gtacgcgaaa ccacatgcgc caggaaggcg
    18301 gtgttttctg cgtcattggc ccactggaaa gctgcgccat tttcaatatg tgcaaccatc
    18361 gcatccagac cggcacgcac ttccgccggg tttggcccac caagcataat cagcacttca
    18421 ccggcagtcg gtgacgggcc gtgtgccgct cccgcataca gcgagcgacc atataccact
    18481 tcgaccatcg cttgtttggt cgcttcgtca gccgcaatgt aggttacgtc gtcagaatcg
    18541 gcagaaatca gcccgaggct acgaatatgc tgcggcaatt ttagttcacg cgcaaattca
    18601 gcgttaacgg aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa atctaaagct
    18661 ggcatgatgc ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt
    18721 ttcgccaaat ccacaatgac ggctgcggct tcaaccggcg gcgtgccgcc ctggtgaatg
    18781 ttagagatac aggtgcgatc ggcctcaacg gtggtcgcca tacgcggcga gtaaacggcg
    18841 tagcaggaga ggctttctga ctgccctaac cccggacgtt cccccaccag caggatcacc
    18901 actttcgcgc cgaggatctc accgatctga tcttcaatct tcacgcgacc ataacgaaca
    18961 aagaatggcg tgccgacttt cagcccggcc tgtttcaggc ccgccatcag cggcggcagg
    19021 atctcttcgt agttgacggt gatggcatca gttgacaggc catcagaaat aacgacctgt
    19081 acgtccggat tagcaacaca ctgtgctttc agcgcttcaa cagcttctgc acacaggcgg
    19141 cggcccatat ccgggcgagt caggtagaga tttttgtcgc taatttcaga gcgtacttcc
    19201 agcaagccct gcgctttcac ccactcttcc ggcacttctt tcagtacggt atctttcgaa
    19261 cgagagtgat cggccaggaa acgcagcagc gcctgagtac gtggacgcgg accggcacga
    19321 ccggtacaaa cgcgggccac ggtgctgcgg cgcagttctg ttaatacgtc tgcgcgatgc
    19381 gggttttcaa caccaatcca cgcttttgct tcagcggaac ctaaatccag cgcacagctt
    19441 tccgaggtca ccggtgccgc acagttggtg gtggcgcact ttgcttctga cggcgcgggg
    19501 gccgcttgtc ccattgacgc catcacgctg cgtacaattt cttcaatctg tttttgatcc
    19561 atgatgtgtt atctccgcgt catcagaaga acagtgacgg atcgcccgcc cgtttggtca
    19621 ggcgaccgtt tgccataatg cccatgcttt ccagccagcg ttcaaactct ggtgacgggc
    19681 gcaggttgag taactgacgc acagtggcgg tatcgtggaa tgcggtggtc tggtagttga
    19741 gcatgatgtc atcacccagc ggcatcccca tgatgtagtt gcagcctgcg gtggcgagca
    19801 ggatcatcag gttttcgttg aggttctggt cagagtcagc gtggttggtg tagcagcagt
    19861 cacagcccat agagatgccg ctcagcttgc ccataaagtg atcttctaag ccagcacgga
    19921 taatctggcg gtcgttgtag agatactccg gcccaataaa gccgaccacg gtgttgacga
    19981 taaacggatc gtaatgacgc gccagcccat agttacgtgc ttccatcgtc acctggtcag
    20041 cgccgaagtt agcgccagcg gatagcgcag agccttgtcc ggtttcgaag tagaggcagt
    20101 tttccccggc gatacgattg aactctgcgc ccactgcgcg cgcttcgtcg agcatcgcca
    20161 gttccacgcc aaactctttc agcccttttt cgctgccaca gatactctgg aaaatcagtc
    20221 cgccaggcgc gccgcgacga atcgcttcga tctgggtggt gacgtgcgcc agtacgcagc
    20281 cctgagttgg gatgttgaat ttgtcgatca cgccataaat ggtatccagc acgctgctta
    20341 agttttccac gtcgtcagtt accgggttaa cgccgatcac cgcatcgccc accccgaagg
    20401 aaagcccttc atagatttgc gcagcgatac tttgcacgtc gtcacgggta tcgttcggct
    20461 gcaaacgggc gctaaaggtg cccggaatac cgatggtggt attggccttt ttgattaccg
    20521 gcattttctt cgcgccgtag atcaggtccg cgttggagca aatcttcgct accgccgcga
    20581 ccacttccga ggtcagccct ttgcgggtaa aggcaatgtc gtccacgctg gtttcatcgc
    20641 tcagcacata ctcacgcagt tcgctgatgc tccagttttt aatctggttg taggccgttt
    20701 cgttaacgtc gtcctgaatc agccgcgtca cgcagtcatc ttcataggca atcaccggat
    20761 tattgcggat gtccgctacg gtcatttccg acaacacctg ctttgccgcc acgcgctcct
    20821 gtgagcttgc cgctgcaacg cccgccagca catcccccga acgcagttcg ttggctttag
    20881 ccagcacctc ttttacatcc ttaaactgat atacattgcc gaacaatgtg gtctttagtt
    20941 tcataagtcg ttccctcagg aagaagtgat gagtaacatt tcttccagca ttcgaacaat
    21001 tctatttcat cgttagccct tgtcctgtaa ggtgttaccc ttaatacgcc cgttacttat
    21061 tctgccggac acaaccgaac gcgccggata catgagcggg gttatcgagt cgttagatcg
    21121 aggtatccgg tttgagcaag acaccactga cagcaaaggc catagaggcc gcccagccgc
    21181 aggacaagcc ctacaaactc actgattcaa tgacgcctgg ccttttcttg ctggtacatc
    21241 ccaacggcag caaatactgg cgattccggt attggataga caagaaggag cggttacagg
    21301 ctattggggt gtatccgctg attagcctca aggaagcccg caaacgcgcc acagagagcc
    21361 gtttactgat agcccaggga attgacccaa tggatttggc acgcaaggag aaagccattg
    21421 atgctcttaa tgctatcgcc agttttaaag ctgtagcaga agactggatg gctaccaagg
    21481 tgagcggatg gagtgagagc tatacaaagc aggtcagatc ggcactagag aaagatgttt
    21541 accccgtgct aggtaagcgg tcgatagtgg atataacggc ccgtgacgtg ctggcgttac
    21601 tacagaaaaa ggagcgcacc gcaccggaac aagccaggaa attacgccag cgtatcgggg
    21661 agatcttcaa atttgccgtt atcactgaac tggttaaccg gaatccggtt gcagatctgg
    21721 atacggcact gaaagccaga cgcccaggcc ataacgcgtg gataccgatt aatgaaattc
    21781 cggccttcta caaagccctt gagagagctg ggagcgttca gattcagacg gcaatacttt
    21841 tgctttgagg acggcagaat tgcgcctgat gcgctgggaa tgggtagatc tgcaatcggc
    21901 aacaatcaca ttgcccgctg aagtcatgaa ggcccgccga ccgcatgttg tcccgctatc
    21961 ccggcaggcg gtcgagctat tacaggacca gtttacccgc agcggataca gtgctttcgt
    22021 ctttcctggc cgattcatgg acaagccact ttcagccagt gccatcctta aagccctgga
    22081 gcgtatcgga tacaagtcga tagccacagg ccacggctgg cggaccacct tttcaacggc
    22141 gcttaatgag tccggcaggt actcaccaga ctggatcgaa atccaactgg cccacgttcc
    22201 gaaaggcgtg cgcggtgttt ataaccaggc ggcctacctg aagcaacgac gggccatgat
    22261 gcaggattat agcgactgta ttgattcaat actggctggt gacggcaatc cgcttgagcc
    22321 ggaatgaata attaagcggg tttccgacct actccaccgg agcagactag ataaatactg
    22381 gaccccttca gaataaatat tcacctgccg gattcgtccg gcttttgttt ataaatcatc
    22441 caatcgtgaa attattagaa ttaaacttac atactagttt actagccaga ttaggctact
    22501 agtatataac atcaataaaa tcatgaggtt agtcaaaagt tgccgtcagg ggtcatgcgg
    22561 gcgtaacatt agatcaacga aataaaaaag cccggaaagg cggcaaccag ttccgggcgg
    22621 tatgagaaac aagctaaagg agaatttctc atgtctgata tgctagcagt agaacaagaa
    22681 acaaacaatg atgttcgcca atatttaaac aaaattaacg agttacggaa taaagcaccc
    22741 aaaaacgaag agactaaaca cgaagaacac acgccagatc attacgaggc aaaacagcag
    22801 gagcaagcct ggcgtggcaa cctgcgctac ctggacacat taaaccgact tgatgaggta
    22861 ttgcctcgca aactgtacga aaggtgggag aaagaacata cagtcaacga cgaggccgtg
    22921 ctacgcgccc tttgctactt tgctggtact ggcaaaaact cacagttggg atggtgcagg
    22981 gttggcaggg gaacaatcga caagcgagcc aggctatcaa aaaacacggt taaaaaatgc
    23041 ctcgatcgtc tggtccacca cttcaaactg gttgagcgta ctgaaggcta tatacccgga
    23101 tcggcagaaa gggaatgcaa cgagtaccag ctattattca agccgtacaa catgaaataa
    23161 cggaatcagg cgggcaatgt tcccgccttt tctttgcctt aattccatga attcatgggt
    23221 gggtcaaaag ttgccgttag ggtaggtcaa tttgtgcctt taggtgggtc aaaagttgcc
    23281 gagtaagaat ttcactatta agaattctag gcgacgggag ttttttagcc tcccggtcgg
    23341 tcacagagag tcgcctagga ttaaaacaga agaatttatt gccgcgcagc gctggatgaa
    23401 tgcaatcatc aataccgctt tggcgcgtca tccttcccag caagctggaa aggcccattc
    23461 actcacgcac aacctgcaaa ccagaccaac agaccatcat ggcgattcgt tcatgggtag
    23521 cgcgggtata tctcaaaggg tgatttcttc ttggtggacc ttcggaagac ttcgagcaat
    23581 tgccagctta aagacagctt agcagtgatg gttatcatca acagctttct gagctaccgc
    23641 acagacacac agcagcagta agcaaaccga agggaatgca aagcaaaagg attcgccaca
    23701 ctgcaagacc aatggttttt gtcacgtata cagaccaggc aagcaacagc agcaaagcag
    23761 cgtatagcca ggtgcagata gtaccatcac acaacgcaca gcaggctgag gtgaacactc
    23821 aagcaacttt atccccgcca ccgaccaccg caaactatcc tgaccgtgcc aaattcgaac
    23881 gtaatgccac ctggaacgcg atggagacac ataagggcta cgcttgctta gctgaaaact
    23941 tcacagcgtc acagcgctat ttagacgcat tagagtcgat gcgatttcat taaatttttt
    24001 agccatccag aaatcggcct gattctgagc cgattgccag ccggactact gaccagatcg
    24061 gaccaatcat ccaaggttgg atgaatgaaa tcacacgcag atcctgccag gctatttcat
    24121 cccaccacac agaacgctga gatctgatat taccgccgca ccattccacg ccagacgaat
    24181 caaaacaggc ttacccttcc aagtggatag ctaaaaatta aggctgtcag aatgcgttat
    24241 attgcgttta aacggcatcg aattttaatg tgcgcaatgg tgccagattg caggcaaaaa
    24301 aaatcccgcc ggagaaagcg gggaagtgaa atgaatcgtt gtagttatag atcacatttt
    24361 gagtaagggt aaccagcccc tgagtaatat tatccctgca ttacgtgaga tgcgcaatta
    24421 ttttttgcta attaaaagtt ttgtaaacat tacagaatat cccctttcac tatatggtaa
    24481 aagatgtgaa acagggacag gaggaatata atattaagta tcaacagtaa tgttggcctt
    24541 taatttcatt cagggcgcgg tgagggtttt ctccatattt tcctcaccgc tactttttga
    24601 ggtgagaaca tgaatgatag cgaattaatg acggttaacg aggtagcagc tttattcggc
    24661 gttacccgtc ggacaatatt ccggtggatg aacaagatta aaggctggcc cgtcccggtc
    24721 agcccgattg actcaagaat taagtttatc cgatctgaga ttttggaatt ctataaaaac
    24781 aaaggcgctc gccaccaatg aaaacagcta ctgagattat caaataacct attgatttaa
    24841 aaagctagct accgcaattt ctgaaggaat aggcagcatg aaaatcaaag caaacacaat
    24901 taaagaaata acatcaatca aagtaaaagg agcagatgcc atgcattcat tagacagtta
    24961 ttttcagcgc acgacagccc ctaaatcagc cgcccaggaa cgccgtgagg aattccagga
    25021 aaaagtgatg cgttctgctg attatattgc ggacaagttc gtggaaacgg ttcggccact
    25081 tgttgatgaa gtagctgata aacttcagtc tgaaatgccg gaagacatgg aaggcactgc
    25141 aaaggcgcga ctcctttttg aattaagtcg tcgtttcggc gtgtcaataa gcacttttaa
    25201 ataatcggag gaggaaagac aatggacgca tttgatgtac tgacaaacaa aggcgatgaa
    25261 ctggtagcac agcgtgataa aaccgccaac gaaggggaac gcgcactgtt aaataagcag
    25321 attaaagcga tccgtatggc ccagtttaaa ttaatcagca atgaagttat cgaaataatt
    25381 aaaccgcaga tcccacaaat cgttgctgac gctattaaag ccgccggact ggagaaacgc
    25441 attgccgggc tgaaaacgaa cgtagagaaa atggagatgc acaaacagag cggtgctccg
    25501 actccggatg agtacttctc accggatgct gagtttatgg cgcaggttga ggaacgtctg
    25561 ggtagctgtc tcaccgagga acagcgtcgc tattttgatg gagtggatag ttcggcaggt
    25621 attgacctga actcgtattt cggtcgtgaa attgagcatt ttgatgccgc cccgtctatg
    25681 ccggagccag aaccagaaat ggccgccgat cctgaagctg agcaacggaa acgcgtattc
    25741 aatgcgatag ggattcggta ctgatcggat aaaagaaagc cgggaattac cccggctgac
    25801 ttaacaatgt gaccaatgaa caaatcggac aagacaaaca ggataagcag tctcgaaaca
    25861 atgttcggga actatattag cacaagtccg gagaaaatca tcatgttggc actacgcatc
    25921 atgcagggca tcgctaagac cctcgcagaa catgtcttag acctgaaaca ttcgccactg
    25981 agcaaacagg ccatgaagcg ccaaacgctt cgtctgtggg ctgaatattc gcttggaact
    26041 atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga ggaaatggag
    26101 ttcatcaggc gtttaattct cattcgcaga gacattcata gccagttaca cagcgtggga
    26161 atcgatatta atgatgggac gggcgattaa ttttcggaca agtactatgc gcgcgcgagg
    26221 agctgaacat gaagcaagat attgcagaca ggctggagat actcgaaggc cagcgggcag
    26281 aagcgaaaca actacgtaag caggccagaa gagcgcacag gaacaacgaa gcagagttac
    26341 taaccaaata catcagtttc accaattact gcatctacga gtgttacaag gaagatgcag
    26401 aggactggct ggattcattg ccagaacagt actgagcaga ataacaccac gtcatgttgt
    26461 tctgaggtga gcatatcacc tctgttcagg tggccaaatt tagtgtgcca cttcagcatc
    26521 accgttgaaa tcacaataac gatttttttg taaatatgct ctcacatatg taggaataga
    26581 ccaggttggc attgaaacac atctaccatg accataataa agtaattttt tgatatgttt
    26641 atggttttat attaattaaa atatatctaa aattgatagt tttaaaattg caaaatccag
    26701 attgtgtttg taatgtttaa tctgattgta ttgagtggcg tttattatga aaaataaatc
    26761 aggtaattct tgttatagag aagcagtcga tattcatagc tcacttgagc tttcatttat
    26821 attgcataaa tatcatttgc gtgtagatga gaagaaatct cgaaatgtat tcagcgccat
    26881 acgtgatgag ctcatccttg atggggattc acgacaaaac ttagctactt tctgtcaaac
    26941 atggcttgat gatgaaatcc gtgagttgct taatttagct attgataaaa acatgattta
    27001 taaagatgaa tatccacaaa tggcggaacc tgaaggtcgc tgtgtgcgta tgctttccga
    27061 ttcgtggagt ttccccgatt cgcgacacac cttaggttgc tcaaccattg gttcatctga
    27121 agctgctatg ctcggccgct tggcgttaaa atggcagtgg tgtaaaaaac gcgaagtaca
    27181 aggtaaatca accgaacctg atttgcgatc ctgtgcaaat atgctggcat aaattctgcc
    27241 gttactgtga tgtgaaattg tgtgaaattc cacttgatga tcatcacctg ataatgaatg
    27301 ctaaagaggt tttgaagcat gtcgatgaaa aaactattgg tgtcgttacg actttaggta
    27361 ttacttattt ttgtaaagca gattgcgtta gtgtttttaa gataagtgtt aaagttaata
    27421 ttagttttta tatttacatc gtaattatat gtttctattt taaaaatatt cgagagtata
    27481 aaaatgtcta atgctaatat cgcacccaaa agtaagaaaa tgacagtggg gacactcgct
    27541 atcatgaata taactgctgt agtaagtcta cgatttatac cttctgaagc agaatacggc
    27601 ttaggtgcca ttttttacta tacctttgct gcaattatgt ttttagtacc tgttgccttt
    27661 gttgcggctg agctagctac aacatatcca caaaaagggg gggcatttcg atggatagga
    27721 gaagcatttg gggctcgctg gggttttgta ggaatgctta tgacctggct acaagtcatc
    27781 ccctactttc caacagtact gacatttggt gcagtctcgg ttgcatttat tgatgttgat
    27841 atgggggtgg cagaatcaat tgccagtaat aaatggtata ttttattttt cgtactattt
    27901 gtttactggg gagctatttg tgttgcatta cgtggcgttg gtattttctc gcaggtatct
    27961 aagtggtgcg gtattatagg gacaattatt ccagctatta tccttgttat tcttggtttc
    28021 tcctatctgt ggttcagcgg taaaccagcg gtgatcccat taggttgggg tgatcttttc
    28081 cctaatatag ctaacttcca gaatattgtt cttgccgcca gcatatttct tgcttatgca
    28141 gggatggaaa tgaatgcggt ccatgttaac gatttagata atccaactaa aaaataccca
    28201 attgcaatta ccatcgcttc tataggtact attgcaatat tcctgctcag tactttaggg
    28261 gtcgccttta ttattcctac agataaaatt agtctaactc aaagtttgtt ggttgcctac
    28321 gatatgctgt tcgaatgggc tggagtgcca tggcttggct ctgtgatggc atttatgcta
    28381 gcaattggcg tactgggtgg ggtggttact tggattgcag ggcctaatac aggtgtatta
    28441 gcgatcgcga aagctggtta tttacctaag tttttccaaa aaaccaatcg tcatggaatg
    28501 gggcaccatc taatgtttgt acaaggtata attgtctcag ttctttctgt tacttttgtg
    28561 ataatgcctt cagttcaagc agcttttcag attttatcac agttaacagt tatgctctat
    28621 ttagttatgt atatgctcat gttcgctagt gctatctatc tacgacattc tcaacctgat
    28681 acaaaacgcc catatcgagc ccctgcttta tttttctggg caacacttgg attcttaggg
    28741 tcttttctgg cttttgtatt aagttttatt ccgcccgagc aaatcccaac tggtagtcca
    28801 accagctatg ttctttattt gttagtatta gttgccctat ttttattaat tccaatatta
    28861 atttacgcct tccgaaaatc tgactggaaa gatccaaact ctgatttcga accattttct
    28921 tgggaaaaaa gaactagtaa ttaaattttc cataagctac aaaagatgag gttaaatatg
    28981 tttagtgctg aatctccaat tggtgtaata gactcaggtg taggtggatt gacatcggta
    29041 aaggaaataa ttaatttgct tcctggtgaa gatattatct attgtggaga taatatgaat
    29101 gcaccttatg gcaaccgttc tgccgatgat atcatcagtt tgactaaaaa aatgctgact
    29161 tttttacagt caaggaatgt caaactagtt gctgtcgcat gtaatactat atcctcaact
    29221 ttggaatccg aagaatacag cggatatgct aagagctttc cgtttcctat cttatccatc
    29281 attgaacctg cagtagaaga tgtgattcgc cagcaatata aaaatgttgg tattatcgct
    29341 accgagttta ctattaaaac ggggtgtcac aaagagctaa taaaaaaatt gaactcaaca
    29401 ataaatgtat ttggagaacc aagcaaaaat cttgcaatgt taattgagga aggtaatctt
    29461 aatgctcctg caatcttgaa tgacatcaaa aaacatgtta accatttaat ttcccttcat
    29521 ccagtaaatg aaattattct cggctgtact cactatccaa ttgttcagaa tttttttgaa
    29581 gcagttgcac cggatataaa atttattaat cctgcacatg atcaggctat ctctattaaa
    29641 aatcacttgg gacagctaaa tttattaaac tcctcaaata taggaacttt gcaaataaat
    29701 actagtggaa gtatggagat ctacaaaacg gtactagggg aattgagcat tacgaaacct
    29761 catacattca gtatcaggca gttttaaatt tatccggtgc accgtaaaac cccgtccttc
    29821 tgaactgcac cccaaaagtt ggacaccaac tgagtaaggt gcattttatg gacaaaccca
    29881 gttacatact gggtagtgca tccaattagt agaacatgtg tttttcgata aacgctccga
    29941 tcactttttc gtggatctca actgagcgtg agaagcagat tgttttacga cccaaccgct
    30001 taatgcgggt gcgtagcgtc agattattac gctcaatgcg ttgggtgaat attttgccgg
    30061 tcagatgctt attcttcggc acctcccggc catagctgcc ccagtcatcg ctggtcagca
    30121 tgccgatgtt gaagggtgta agcagtgcca gtagctcccg gcacgtttga tcggttcggg
    30181 gaccaaaagt gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac ccgagccagt
    30241 gttgccgggc tttactgcca acgtagctcc attgctcatc aagctcgcag ataaacgcca
    30301 catcagcatg ggcaacaggc gaagaagtta ttcgctttgg cgcgagtttt ttaaagtccg
    30361 gatgacggtg ttactaccaa ttttcagtgt cctggcggta tcgcgaaccc cggcaccatt
    30421 aaaggccatt tcagttatca gctctttcat acccggctta cgtgcttgat aagtataagt
    30481 gaactgaaac acacggtggc agtcacggca gcgaagtctg tcacggcctt tagggttctg
    30541 accatggcgg taaacctgag ctgactgaca acggggacaa tgaatattaa cgctggtcat
    30601 gagataacct caaaagcccg tattatacat cagattcaac taattagatg catcacccat
    30661 ccagcattat aaaacatact aacaataact aaattataat aatcacaact tacaatcaaa
    30721 taataaatgc ataagattaa caatgatttt ttctggttat atttatctaa aaaagcagaa
    30781 tattatgtaa cttattattt tctaagcgaa aatttattgt gcggtcagac taacaaactt
    30841 gcgtaccacc cgaagaacat gcgaagattt aagtattctt tcttttttgg tttttatttt
    30901 tcattttctc ttttcttctc tttccggttc tcttcttttc tggttttggt tttttccttt
    30961 ttatttttat tctttcttca gggttatgct aaaacaccaa aacatttttc accacaaaag
    31021 catccaatca tcttgccaga ccaatcacct taaccggata cactgccgga tataatccca
    31081 ctaacaccca gccaccaagc accatacctg ataactaccg tattcaggaa ggaaatgcga
    31141 gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag aggcgtacca atgtcgatat
    31201 agtcccccgc gcggacaatc acttcgtcaa tgactgccaa cgggagttgt tgtagctgtg
    31261 ggcgcaacaa catgcccaga gctttaccaa agtcctgccc ggccaccacc agcaggggat
    31321 gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt gatgaccgtc agtaccgcgg
    31381 cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta cgcgtcagtt ttgggatcaa
    31441 gatccagctg aagcagcgcc tgttgccagg cactcaccag atccgtttca tcaatcggga
    31501 tcgccaccgg caaattgcgc agcggcagtt gtacgccctc cagccagatt gtgctgccag
    31561 agagcgaaag ggtatgtgca cccgcgccaa tcaccgtggc gcgtacggtt tgcgccggaa
    31621 actgcacatt catctcacgc aggcgcggat ggtcatgcag cgccgttgcc agaagtgggc
    31681 caatatcggc aaaacagaac gggtcggcgg gctggtggcg ataacattcg cccacgcctc
    31741 cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag caaaccggtt tgcatcaatg
    31801 cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac aatcagcgcc gccatccgcc
    31861 gggtaacctg cacaagctgc gcgccggtca gcgaacgggc gtcagtgcct gcaccgaagc
    31921 actcatccac aatcatctgc cccggtttat gagcgtaaac cacgcgcccc tggctgtcgg
    31981 tttccagcag gcgaccaccg acgttgagac aggcagtgcc gctgattttt ccggcatcga
    32041 acagggcgta gttcgcggtg ccaccgccga tatcgatatt cagtacccga cacagccgtt
    32101 gttcagaaag ggtttgcgcc ccggctccgt gaccggcgat cacggattcg aggtgcggcc
    32161 cggcgctggc gacgacaaaa tcgcccagcg actgagagag cgccatcacc gccgggcgag
    32221 cattgcgggt tttcgcgctt tcaccggtga tgatgatggc accagaatca acgctttccg
    32281 gcgcaacgcc cgcagcctga tattgctcta aaatcagcga tttcagttcc gcttctttta
    32341 aaccaccctg tttatcgaca ggggtaaaga acaccgggct ttgccagcta atttcgcgtt
    32401 taatgaattc gtagcgcggc acctgcgaca ccgccgcacg gttaaccagc tccaggtggg
    32461 agaaaatcac ctgggtggtg gtggtgccga tatcgatacc gacgctcaat agctggcgag
    32521 tgttcacgat tgtgcctccg cttcggtttt agtcgcggtc gcgtcttctt ttggcaccag
    32581 catcatcgcc acgccaatcg ccgttacgcc gccgatcaac ttgccgacaa tcatcgggaa
    32641 gatcatggcg ttcatgttgg cagcggcgaa gcctaagtgg tcgcccaggg cgaaagcagc
    32701 ggaaacggcg aaggcgcagt tgatgacttt gccgcgggta tccatctgcc tcatcatgcc
    32761 gaacatcggg atgttgttgg caagcgttgc caccatgccg gctgccgcga tgttgttcat
    32821 attcagcact ttaccgacgc tcatcagcgg tttttcaaac cagcgggtca gcagcagcac
    32881 cattggatac gcccctaaca gaacgcagga gatagaaccg ataacttcaa tggcgcgcat
    32941 cacttcaccg ggtttatcgc caggggccat aaagataggg tccagaccgg ggatcagttc
    33001 ccagccaagc agaaacttaa ctaccgcggc agcaagaccg agggtgatca atgcaacgag
    33061 gaatttggcg aagatctgga agccgttgat cattttttcc gggatgaatt tcagccccag
    33121 cgccaccagt accgcgacga taagcaccgg gatcatgttc atcaggatga gcgcaaaggt
    33181 gaattccacc ggctggccgt tgatctgtac accggagtac atagccacca ggccaccggc
    33241 gatacagcca attggaatgg tcacaatgcc cgccagcacg ccgagcgcca gataacgacg
    33301 atcagaaggt tcgataatgc cgagcgccac cggaatggaa aacacaatcg ttggccccat
    33361 catcgacccg agaattaacc cagagtatag ccacgctgct acgtcgccgc ccgccagctc
    33421 tttggcgagg aagaagccgc ccatatcgca cgccagcagt gttccggcga acattgacgg
    33481 gtttgcaccg agcatttcgt aaaccgggat aattaccggc ccgagtacgt gagccagtac
    33541 cggtgccagc gcggtcatac cgaccatcgc cagacccaga gcgcccattg ccataaagcc
    33601 ttcttcgaac tgaccgcctg atccttcgat acttttaccg aacttaccga ggaaacgagc
    33661 agaaccgccg aactgcgaca ggatcctgtc tacggcagct atcagcataa agaacatcat
    33721 gatgtacatg atgatttcgt taattcccat cgcctttact ccctgttagt tgttatttat
    33781 tggcggatgc ggcgtaaacg ccttatccgc cctacatgtg caatcccgaa ggccggatca
    33841 gacgcggcaa gc
//
LOCUS       DADLSB010000027        33432 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.027, whole genome shotgun sequence.
ACCESSION   DADLSB010000027 DADLSB010000000
VERSION     DADLSB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.027"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1327..2106
                     /gene="ipaJ"
                     /locus_tag="JN331_001278"
     CDS             1327..2106
                     /gene="ipaJ"
                     /locus_tag="JN331_001278"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY7259468.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3052..3981)
                     /locus_tag="JN331_001279"
     CDS             complement(3052..3981)
                     /locus_tag="JN331_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY7259469.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4198..4434)
                     /locus_tag="JN331_001280"
     CDS             complement(4198..4434)
                     /locus_tag="JN331_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7259470.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4438..6339)
                     /gene="ipaA"
                     /locus_tag="JN331_001281"
     CDS             complement(4438..6339)
                     /gene="ipaA"
                     /locus_tag="JN331_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY7259471.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSLGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQPDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6348..7346)
                     /gene="ipaD"
                     /locus_tag="JN331_001282"
     CDS             complement(6348..7346)
                     /gene="ipaD"
                     /locus_tag="JN331_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY7259472.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDTLSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKNALEELKKKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7397..8488)
                     /gene="ipaC"
                     /locus_tag="JN331_001283"
     CDS             complement(7397..8488)
                     /gene="ipaC"
                     /locus_tag="JN331_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY7259473.1"
                     /translation="MEIQNTKSTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIAHLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSKNSTASQIAGNIRA"
     gene            complement(8508..10250)
                     /gene="ipaB"
                     /locus_tag="JN331_001284"
     CDS             complement(8508..10250)
                     /gene="ipaB"
                     /locus_tag="JN331_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY7259474.1"
                     /translation="MHNVSTTTTGLSLAKILTSTELGDNTIQAANDAANKLFSLTIAD
                     LTANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KSFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10256..10723)
                     /gene="ipgC"
                     /locus_tag="JN331_001285"
     CDS             complement(10256..10723)
                     /gene="ipgC"
                     /locus_tag="JN331_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY7259475.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10781..11407)
                     /gene="ipgB"
                     /locus_tag="JN331_001286"
     CDS             complement(10781..11407)
                     /gene="ipgB"
                     /locus_tag="JN331_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY7259476.1"
                     /translation="MQILNKILPQVEFAIPRPSFNSLSYNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDNVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11423..11812)
                     /gene="ipgA"
                     /locus_tag="JN331_001287"
     CDS             complement(11423..11812)
                     /gene="ipgA"
                     /locus_tag="JN331_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY7259477.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11825..13309)
                     /gene="icsB"
                     /locus_tag="JN331_001288"
     CDS             complement(11825..13309)
                     /gene="icsB"
                     /locus_tag="JN331_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY7259478.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEE
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13623..15239
                     /locus_tag="JN331_001289"
     CDS             13623..15239
                     /locus_tag="JN331_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY7259479.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNPKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTKYLNNKNWEPINKNINY
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESQHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRRELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKGLNSKRGEPTKLLIRNSDGLLQEVNVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKDIINKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHDTGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15249..15611
                     /gene="ipgE"
                     /locus_tag="JN331_001290"
     CDS             15249..15611
                     /gene="ipgE"
                     /locus_tag="JN331_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY7259480.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15611..16069
                     /gene="ipgF"
                     /locus_tag="JN331_001291"
     CDS             15611..16069
                     /gene="ipgF"
                     /locus_tag="JN331_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY7259481.1"
                     /translation="MSRFVFILLFFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESR
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16078..17193
                     /gene="mxiG"
                     /locus_tag="JN331_001292"
     CDS             16078..17193
                     /gene="mxiG"
                     /locus_tag="JN331_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY7259482.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICEANKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17201..17452
                     /gene="mxiH"
                     /locus_tag="JN331_001293"
     CDS             17201..17452
                     /gene="mxiH"
                     /locus_tag="JN331_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY7259483.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGELTLALDALAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17465..17758
                     /gene="mxiI"
                     /locus_tag="JN331_001294"
     CDS             17465..17758
                     /gene="mxiI"
                     /locus_tag="JN331_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY7259484.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVGNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17764..18489
                     /locus_tag="JN331_001295"
     CDS             17764..18489
                     /locus_tag="JN331_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY7259485.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVTLLVWAFKTGWFKRNKI"
     gene            18486..19013
                     /locus_tag="JN331_001296"
     CDS             18486..19013
                     /locus_tag="JN331_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY7259486.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18955..19650
                     /locus_tag="JN331_001297"
     CDS             18955..19650
                     /locus_tag="JN331_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY7259487.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIINTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19643..20050
                     /locus_tag="JN331_001298"
     CDS             19643..20050
                     /locus_tag="JN331_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY7259488.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFDAGREADDSTW"
     gene            20034..20462
                     /gene="mxiM"
                     /locus_tag="JN331_001299"
     CDS             20034..20462
                     /gene="mxiM"
                     /locus_tag="JN331_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY7259489.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20592..21224
                     /gene="mxiE"
                     /locus_tag="JN331_001300"
     CDS             20592..21224
                     /gene="mxiE"
                     /locus_tag="JN331_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY7259490.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21211..22911
                     /gene="mxiD"
                     /locus_tag="JN331_001301"
     CDS             21211..22911
                     /gene="mxiD"
                     /locus_tag="JN331_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY7259491.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22930..23997
                     /gene="mxiC"
                     /locus_tag="JN331_001302"
     CDS             22930..23997
                     /gene="mxiC"
                     /locus_tag="JN331_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY7259492.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24010..26070
                     /gene="mxiA"
                     /locus_tag="JN331_001303"
                     /gene_synonym="virH"
     CDS             24010..26070
                     /gene="mxiA"
                     /locus_tag="JN331_001303"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY7259493.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26083..26484
                     /gene="spaK"
                     /locus_tag="JN331_001304"
     CDS             26083..26484
                     /gene="spaK"
                     /locus_tag="JN331_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY7259494.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26488..27780
                     /locus_tag="JN331_001305"
     CDS             26488..27780
                     /locus_tag="JN331_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY7259495.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27873..28211
                     /gene="spaM"
                     /locus_tag="JN331_001306"
     CDS             27873..28211
                     /gene="spaM"
                     /locus_tag="JN331_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY7259496.1"
                     /translation="MEALDKRIIYFQQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28198..29076
                     /gene="spa32"
                     /locus_tag="JN331_001307"
     CDS             28198..29076
                     /gene="spa32"
                     /locus_tag="JN331_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY7259497.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29070..29951
                     /gene="spaO"
                     /locus_tag="JN331_001308"
     CDS             29070..29951
                     /gene="spaO"
                     /locus_tag="JN331_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY7259498.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29956..30606
                     /gene="spaP"
                     /locus_tag="JN331_001309"
     CDS             29956..30606
                     /gene="spaP"
                     /locus_tag="JN331_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY7259499.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30621..30881
                     /gene="spaQ"
                     /locus_tag="JN331_001310"
     CDS             30621..30881
                     /gene="spaQ"
                     /locus_tag="JN331_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY7259500.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30881..31651
                     /gene="spaR"
                     /locus_tag="JN331_001311"
     CDS             30881..31651
                     /gene="spaR"
                     /locus_tag="JN331_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY7259501.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31658..32686
                     /gene="spaS"
                     /locus_tag="JN331_001312"
     CDS             31658..32686
                     /gene="spaS"
                     /locus_tag="JN331_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY7259502.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32699..32941
                     /locus_tag="JN331_001313"
     CDS             32699..32941
                     /locus_tag="JN331_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259503.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10438 a         4840 c         6688 g        11466 t
ORIGIN      
        1 ttaccccctg gatctgattt caggcgttgg gtgtggatca ctattgcacc gttcgtgaca
       61 cttgttgact gcctgtgcgg catctggtat caaggtggcg tgaagccggt tcaggttctc
      121 atgcggaacg ttggaaccta tatccccccg ataccaaagg cgaaatgtaa gtgacaaaat
      181 gcaatgaaca cgttctccgt taactgagga gcatggtgat aaataaaggt aaatgcagaa
      241 ggtgtagagt ctcgcagatt tctgacatca tagagcctaa cattagatgc ataacgctac
      301 agattgtaga atctctacag gtgaaatata cactggttgg cgtttatgtc gcaaaacatg
      361 tcattcaggt tcatctcacc aataaggata tgagtgaagt ggaggataag tgagtctgct
      421 gtcagagttt tctggtgtat gtcagtaact gcgaaccatg tctgcttgat atgggagctt
      481 atggaggctc tctgtacaag gttagggagc taacaaagca gggataaaaa gtccgcctga
      541 tgcaggcacg gttcgttaaa tcttttgcca ttggtaacaa aatggtgcga tgaatgttca
      601 ggctatcttt atcttgatgg tggttcagct gtttggtaaa gaaatcgctg taacaataga
      661 agaactgcag gcagtactgg tcccacatcg tttgctcagt caactggtga agttccgtag
      721 cgcgtaaatt aatgctttgc atgaaacgtt actggagtgg atgaaagaat acctgcatat
      781 atcattactg cgttggatta ccagtacaac agattgaatg aactggacat gttgatagac
      841 ggcattgaaa agcagcttat aagcatagtg cggcagagtt aattggagag cacttcgcct
      901 tctgagtatt aagtaagcat ggtggtacct gcggatacta tctattcact gtgctcggtc
      961 ggcagcctta ctgatcaagg agttggggga gttgataaca aagttgaaaa aacagctctc
     1021 aaccaaggtg gttattgtaa caatagcaaa taagctggtg tgacctgtat cacaataacc
     1081 gattatggac gtaaatacga caaaaatatg tcagtatcag gcggtattaa ttgctgatgc
     1141 tgatggacat taactcttaa taaaagggtg gatgctgaaa ggttgttatg gtgacggacg
     1201 tgatgacgac ggaaaagagt atgaggtttt ggatttaaag ttcgttcggg gaataaggac
     1261 ccggtcagct aattctcata gtagttcgca ttgctggatg caatactgtg ctgcaattac
     1321 tcgtcgatgt cggaacaacg gaagccttgc aaacggggat gtattcatac aggtgttatg
     1381 ttatatgggg ttttattgca gggggctata ccaagagaat atatgatatc tcatcagact
     1441 gatgtgaggg taaatgagaa tagagtaaac gaacaaggtt gttttttggc gaggaagcag
     1501 atgtatgaca attcttgtgg agctgccagt ttactctgtg ctgcaaagga attaggggtt
     1561 gataaaatac cacaatacaa agggtctatg tccgaaatga caaggaagag ttctcttgat
     1621 ttggataatc gttgtgagcg ggatttatac ttaataacta gtggtaatta taatccgaga
     1681 atacacaaag ataatatcgc ggatgccggt tattctatgc ctgataagat agtcatggct
     1741 acgaggcttc ttggattgaa tgcttatgtc gttgaagaaa gtaacatttt ttcgcaagta
     1801 attagtttta tttatcccga tgcaagggac ttattaattg gcatgggatg taatatagta
     1861 catcaacgag atgtgttatc ttctaaccaa agagttttgg aagctgttgc tgtatccttt
     1921 attggagttc ctgtcggcct ccactgggtg ttgtgtagac ctgatggaag ctatatggat
     1981 ccagctgtag gagaaaatta ttcttgtttt tcaactatgg aattaggtgc aagaagaagt
     2041 aattctaact ttataggata tactaaaata ggtatttcca tagttataac taatgaggct
     2101 ttgtaaattg tcgtttttta tctgtgaagg agcttgaata ttttggggcg gggggcataa
     2161 caaatgatat tactgagtac aaatttagct atattgggcg gattagttgt gtgggaagac
     2221 gaaatgtttc tcttgaaata agtgaactcc ctcaaggaat gattgtgtgc ttaaatattt
     2281 aagtgctggt aagtttgaga aggctgacat attttgcaga gcaaacagtg attgacttgg
     2341 atgatatttt tattttagtc actcgttgta attggagtat gctttatttt atttgaaaat
     2401 agatgtattt tatacccgca atttaaactg taagccctat gaggggtatg gatattataa
     2461 gttactcttt gactaagcat aacttttggg gttagaaaaa taaatctctt gttaataatt
     2521 aatatgattt taaaatcaaa cttgtttata attataaaat aatgtaagtc ggtcatttta
     2581 tgggaagatt aggagaataa aacagtaatt atatttgaga aatctgctaa tatattctta
     2641 aagctttttt gatactgtga acgaagtctg ttaatggtgg attttttgtt tatcaccatg
     2701 taaaaaaatt gagtagtaaa agcgttgact gctaatcctc gctgttttta ttgaaacgat
     2761 taaaaatacg ctgaatattt tcggccagtc actcatcttc acataaaatt ttgaccatca
     2821 tgccgcatcc cttagttgca caattcattt tcaatgatat gaatattact aatttcacca
     2881 gataatgttc tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt
     2941 gcgtggggat tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc
     3001 aacgaagatt tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga
     3061 cgatagatgg cgagaaatta tatcccgaat agcttcatct atttctctct gaactgtttt
     3121 gtttatttta gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat
     3181 gtaagtccgt ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt
     3241 tctgtttttg cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa
     3301 atttgtatca aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc
     3361 attagccttt tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag
     3421 ttctgacgcg attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc
     3481 acgagtgact ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc
     3541 tgatactttc agaaaattaa gaccaatacc aagttctcgg atgctatgct cttttgctgt
     3601 ttgtatatcg tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag
     3661 aacttcaaga tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat
     3721 ttcaattcta ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt
     3781 ttttatgata tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt
     3841 ttggttgacg aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga
     3901 aactttatta ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa
     3961 gtcgttgcac aaatccacca tatcacaccc tgtttattca tattgattta tattgacaaa
     4021 agttaaatgc agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt
     4081 aaaaattact attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa
     4141 tattcttgtg gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta
     4201 tattgatttt gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc
     4261 taatgcagat tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat
     4321 cagagaaaga gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct
     4381 gtacgctata ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta
     4441 atccttattg atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt
     4501 atatatatta ttgttttcag ctgtaatatc attgttattt tttaaatcag atatatcatc
     4561 tgtaagtaat tctgcagagg tatcgtcaat ttttgacaat accttgctga gggcgctaga
     4621 tacttctttt gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc
     4681 tgttacgtag tttattgtat ttgcatctgt aacttttaat ttgttgttta ttctgtgttc
     4741 tgttgtggat tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga
     4801 tttagttcca atgagagata taagattact tagggagttt gttacttttt ttgaagcttc
     4861 aaatatcgtt tcccgtgtta aatatgtgtc tccatttctt gataaaaaat caggctgaag
     4921 tacttttgaa tcagtgtttg agttgaattt attgatttgc gaagctactc tatgagaaag
     4981 agaagcgcta cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg
     5041 gtggagaggg ggggtggttg aagagttctg tatgttcaga ggcctgtgtc ccagagaaag
     5101 agagttattt gcagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc
     5161 cgaattatct ggagtatggt tgaaaacctt ttctaaagct gaattatttg caaatgtctg
     5221 gacagtatct gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg
     5281 tggatttagt aatgagtttc cgttacggga taatagatcg tttgtctgag aagtaggaga
     5341 ctgtgcatca ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac
     5401 tctattatcg taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt
     5461 tttctcattg gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag
     5521 gccatcttgg ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct
     5581 caacagctca ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa
     5641 tcctttacta tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc
     5701 ctgtgcgtca actaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa
     5761 ttcagcaaga atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc
     5821 caatgataaa gcttcaccag atgtgtgttg ttgtgctaat agtgcaagtc tgtgctcaat
     5881 gttttgtcgt aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc
     5941 ttgggataat ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc
     6001 cttttcgttt tcaaaattaa gaccattttg atgtactaag gagtacaaaa catttgaaaa
     6061 ttcgagaatc tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac
     6121 atcttctttc cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc
     6181 aaagttttct ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat
     6241 atcggatatt ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata
     6301 taagaatgtt ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga
     6361 aaaagtttat ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta
     6421 tcaaaaatac tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt
     6481 gtttcatctt cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca
     6541 acctcgccat ttccacctag attatctaag ctttttaaca tattgtctat tggggtcatg
     6601 tttatattga caacatatcc cccgtttttt tcagatacct tgccgattgt tccacctaat
     6661 tctgtaagcc atttatttgc ttgttcctga ctaacagtat tatttgctgg atatagcggt
     6721 ttatctttat attttttctt gagttcttcc aatgcgtttt taagcgagtt gacttgtaat
     6781 ttcacggagt ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca
     6841 gcgctaaaat cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga
     6901 tactgttcat taatatcatt gatggagttt gcaattttag cccacagttc tctatgagat
     6961 atcatttgat ctccatcaag ctcggcttct ttcggggctg aatgtaataa ttctcttgcg
     7021 tctttattaa ttggatattc gttcctggaa agagtatcca atagttgagc ggatttattt
     7081 acatccatgc taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac
     7141 gttttttgtg aaagctcttt ctttaatgcc tgatttgttg ttctgatatt atgaagggtg
     7201 tcgttgagca tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa
     7261 ttaactgttt cggttgatga accgttggta ttgtttggac tgaatgatga ggtggaaata
     7321 ctattagtca gagttgttat attcatggtt atttccttac aggaagagcc atatattatt
     7381 atggctcttg gatatattaa gctcgaatgt taccagcaat ctgactggct gtcgaattct
     7441 ttgattgggt gatgctgtca attatattca ataatttttg tattaattga tttgtcgctt
     7501 gggatgcttc tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga
     7561 ttagttgttc ttcttcttca agagcagatg cataacgccc tcctgatgtg gatatattac
     7621 cagcaactgc tgatgtgtcc attgttgcac ggccaatgtt ttgtttttgc tgcgcagaaa
     7681 gggtattgag ctcgtaagtt cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg
     7741 gagaactaag agaagtttta tgttcagttg acagggatat attatctggc gccagtttat
     7801 ttttacctaa aaattttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt
     7861 gtttatttaa ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa
     7921 ggttctttct taaggctcct ttttggtcgc taatccctga atgcgttttt ttggcaccga
     7981 tacccgttat acctacttgt gtgactgctc cagtaatgct tgatgatagg gctgccaggc
     8041 cttgccgaac aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt
     8101 gagagcccaa tttagtttct gcagtgcgga gagcagaaag tagaacggct acactaataa
     8161 ttaaagaaac agcattagaa gaaagactgg aaatatccag agtgttctct ggagtaaggg
     8221 tgtgactcat ctctgaaaga tctttcatct ttttgtcagt aaggtgagca atgatttctg
     8281 aatcatgctg aacctgctct gataacttta aaagttgatc atcatttaat gtggttgtta
     8341 atacgggatt tttaccgaca ttaagtggaa tatgcgctgc aatctgctga taattttgtg
     8401 atttttgtgt ttcggaagaa ctttgagttt gttttgtaga tatatctgta tataaaatct
     8461 gggttgattt tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt
     8521 tgttgcaaaa ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct
     8581 aatagatctg caattacttc ctgcaattgg ccgaattttt ctattgcttc actgatatat
     8641 tttgacagtt gttcaacttg atatttcgat aatgtcaggt ctgctagatt tgtcgacgcg
     8701 ctgttctgga aaacagcaga agcgacactt cctcccgctt gtgtggcaga gttaacactt
     8761 tctcctaata aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca
     8821 tcacccgctg caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc
     8881 agttgagaag aaaaactctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt
     8941 attttgccaa tattctctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga
     9001 acgactgctg caactaggac aagagcgcct gcgattgccc ccagaataga gccaatcatt
     9061 ttggcttttt tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg
     9121 agtttgatta agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag
     9181 gaattgccgg tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct
     9241 gccagtgcta gagaggctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa
     9301 agtgccccaa gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt
     9361 acttcagcag catactcatc agattttctc tccatttcag tttttcttga ttcttggaga
     9421 gactggaata gagccagatc attttttaaa gattcttcat tattttttcc aactagttga
     9481 ataaaggttg ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt
     9541 gatagctgtt cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg
     9601 agttgcattg atttatctgt aagtttgcta tgaattgaca gggttttctg ctcaatcaat
     9661 gtcctgtctt taactgctgc gtcttttttg atagtgagtt gtatttcttc ccggcttaat
     9721 tttttctttt ctggtgaatc tggggcgagt tcggataatc ttgtttgaat ttggttaatt
     9781 ttattttcta ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca
     9841 tagtctctgg ttagtccttc agtttcagat agaagagtat taattttatc ggagaattct
     9901 aggttttttt gctgtcttgc ctgttgctga gacttccaag cagtaatttt atttgttaat
     9961 gcagttaaag atttttcacc gagtatttga ataaggtttc caattaaaag cgttagttgg
    10021 gaacttgcat ttaatgactt tggtgcttta agttcaggga ttaatatatt tgaagttgag
    10081 tgtgcattag ttgtattaat atttttgtta gcagtaagat cagcaattgt aagagaaaat
    10141 aatttgttag ctgcatcatt tgcagcctgg atagtattgt ctccaagctc agtggaagtc
    10201 aatattttgg caagagacaa accagtggtt gtggtactta cattatgcat aataattact
    10261 ccttgatatc ctgaattgcg tccaagtatg attgtgcttt tatttttaat ttttcatcat
    10321 tgctgtgttg aattacgagt tcgaagcact ctttggcttt caagggggct ttcaaccgaa
    10381 gctggcattg tccagtatgg aataccggtg tatagtcatt ttttcctaat gcaaaagcga
    10441 cagcataaag gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc
    10501 ccataatgta gtctacattg taaaagtcgt atatacataa aaatctgaag aaaacttcag
    10561 cttcctctat tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca
    10621 tatcatcagg aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta
    10681 ctgcagtaga gatgctttca ttttcagtga tatttaaaga cataaggtct cctttttgtc
    10741 tgctttactt taaaaagtat aagatttaat ataaacagat ttaatttgta ttgctttgac
    10801 ggtatacggc ctttagaacc tctgcgctta tttctatttg taaaactttt gttgattcat
    10861 taaatatttt atcgtagacg gaactggata ctagatttgt aagatccttc atttgtgtgt
    10921 taaacaaatc catgtcactg ctatagcgtt ttaaaatctt tgctctgagg ccagatgagg
    10981 caatctcgtt tagtgctgta tgactgatag atgtttgtgc tgatttttca tcaagtttta
    11041 tatccgggaa ttttttttca atagctgcaa atatgttttt tttgttatct gaggtcaaaa
    11101 caactccatt gtgtatggcc atttcatcta tacttctgtt tatcattgaa gagacataag
    11161 aggttcgctc ttggctcatc caacaaaata gttgattccc cgaattacta tcttttattt
    11221 tgtctatgac gttgtccctg attttattta tattcaccgg gcatccaaaa ttcttctgag
    11281 gaaagcgttg ttttaagtta aaaacagata atattttctt tacaagctta ttgtaactta
    11341 acgaattaaa tgatgggcga ggaatcgcga attcaacctg tggaagtatt ttgtttagaa
    11401 tttgcatgat accccctata tgttagttca cttctgaagt gatgtttgca atggttagtt
    11461 taatacaaaa taaaaattca tacaaagatt caatgagttc atcaggtgaa agatattttt
    11521 tgtagtgttt cttggtcaaa acacattgat gagtatcagg gagaagacag atagaagaac
    11581 cataatctct actcaaagag tagttaagga tgagtatgtg ttctggatta ataatatttt
    11641 ctgtaaaaag ttcatgttca ttaagaagtc cggtcaaaaa gatgtattca tcattatctg
    11701 ttatacaaac aggtatttgt tcttcgagca atataaaggc ttgattttta tcgttaaaat
    11761 caagctttgc acctgttatt tcataaagtt tatcatatag tttgcgacac atttctatgg
    11821 ccttctatat attagaatga gagttattca ataaaagctt attgatctgt aataagtcgt
    11881 gaatgtaaag tttggttcca agatctggcg atttaagaga attgtaataa tcaatagttt
    11941 ttattgactt ttcgataatt tcttcttttt tatctgttgc tccggttttt aattcgttta
    12001 atacacgaga catattgctg ggtgctgatt ttactaagta tgagtggcaa gcgttgatga
    12061 ccgcatcaaa gtcttctgaa gatacatttt cagtaggatg tttgtaaagt gaaattgata
    12121 aaggcttgaa tgtttttggg gtgtggataa ggttaatttt attgaaactt ttaagatagc
    12181 tacgtcgtaa ttgagtcaat ctggaaagat caaaatcaga ttcaatatac ttcattatct
    12241 cgttataaga ctgattgaga ctttcaatgc gttgcctaac tttatttgca tacgcgtata
    12301 cattgttagg tgtagcaacc aatttaacgt cagggagtgg aaagtatatt tcagaatttc
    12361 ctgctttaag aacattaagt gccataccag cacagttttc agatttactg attaatttat
    12421 aatttgccaa ttgtctgaaa gcatctgctt tcttttggca tataaatttt gacatgtttg
    12481 tttcatcaag gccaaaaagg ttatagctga ttttaccatc ctttgtttta ttatcacctg
    12541 ataatggaat gtatacttta tcagcactga ccccccaata accatagggg tctttttttt
    12601 gattagctct gggaaaaaat atttttttgt tgttattttg atcttgtttt gaatttaata
    12661 agtttctaac ctcttggcct gcatcaagtc tttcggctgt ttggtctgaa atcatattta
    12721 gtttgtcttg tttataggta tcgacactgt aacctgagcg tttttcaaaa aggtagttta
    12781 tttctgtatc ttttgtaaca ttcccgccgg gataccagct gagataggta tgagttggtt
    12841 cctgtagtaa attgtttttt atactgatac tggtatgccc taaatgggtt atgcctctat
    12901 ttccccagca acgaaaaact aaatgtcttt caagattttt ggctgtaccg atgccatgaa
    12961 aacgctctgc ggtattcaac attaatttga tgtctttacc tgtaaaagaa tgttgaattg
    13021 aatttttgac tgcttcatag gcggcaaacg cactttctgc agatttttcg ctagctaatg
    13081 tcttgaggaa aagcaataag gatttttgat tcgctagtcg ataatttttt agttgttcat
    13141 ttaatatctg tgaatttatc tttgcattaa tagtgcttaa tgttctgaaa aagagatcct
    13201 ttaggttgaa tttctgtgct accaatatag gtccatgctc tgtatcttca acgcgtatag
    13261 gcccttttgt attgcttgcg tcaatgaaat tgctaatttt gagaatcata ctttattaac
    13321 tctccattac gtggtgattg tggggatgta aaatgtattt gatatataat atattcaatt
    13381 aataaattta gaaacttgag cctgttaaca taatcaaatt ttcttttgtt gtacataata
    13441 tgtacctcgt gagcatatgt agtgctcgtt tcatcatgaa atcccacaag ttaaagtgtc
    13501 tgatatatca ggctcggagt gttatagaaa aagagagaac cctgttgaat aagtatcttt
    13561 tttgttgtaa aacgaggtac aaaactgcaa tcaatgcccc atttatgacg gaggataatt
    13621 aaatgcacat aactaatttg ggattgcatc aggtttcatt tcaaagcgga gattcctata
    13681 aaggcgcaga agaaaccgga aacccaaaag gtgtaagcgt gatttcatat caaagagtta
    13741 aaaatggaga gagaaataag ggggtcgaag cgcttaatcg attatattta cagaaccaaa
    13801 caagcttaac agggaaaggc cttttgtttg cgcgtgacag agcagccgtt ttttacgagg
    13861 caataaagtt ggcaggtggt gatacgtcaa aaattaaagc catgatggaa caattagata
    13921 cctataaact tggtgaagtt gataaaagaa atattaatga gcttaataaa gtaataagtg
    13981 aagagataag agcacagcta ggcattaaaa ataaaaagga attgcaatcg gaaataaaac
    14041 aaatatttac taaatattta aataataaga attgggaacc gatcaacaaa aatattaatt
    14101 atcatgggaa aaattatggt tttcaattaa cacccgcctc tcatatgaag atagggaata
    14161 aaaatatatt tgtcaaagag tataatggaa aaggaatttg ttgtgcttct acgagagaga
    14221 gtcagcatat tgcgaatatg tggctttcaa aagtggtgga tgacgagggg aaggaaattt
    14281 tttcgggtat cagacatgga gttatatcag cctatggatt gaaaaaaaac tcatctgaaa
    14341 gagctgttgc tgctcgtaat aaagctgagg agttagcaag cgcagcatta tatagcagac
    14401 gagaattatt atcacaggct ttgtctggta aaacagtaga tttaaagatt gtttcgactt
    14461 ctcttctgac gccgaccagt ttaactgggg gagaggagag tatgttaaaa gatcaggtaa
    14521 aggcattaaa agggttaaac tctaaaaggg gggaaccaac gaaattatta attcggaata
    14581 gtgatggtct tctgcaagaa gtaaacgtta atctaaaagt ggtgacattt aactttggtg
    14641 taaatgaatt agcgctaaaa atgggcttag gctggaggaa tgttgacaag cttaatggtg
    14701 aatcaatatg ttctttgttg ggggataatt tcctcaaaaa tggtgtgatt ggaggttggg
    14761 ctgcagaagc tatagaaaaa aatccaccat gtaagaatga tgtgatatat ctggctaacc
    14821 agataaaaga tattataaac aagaagttac aaaaaaatga taatggagag ccgtataaat
    14881 tgtcacaaag aatggctcta ttggcttata ctataggtgc tgtaccttgc tggaattgta
    14941 agagtgggaa ggacagaaca ggtatgcagg atgctgaaat taaaagagaa ataataagaa
    15001 aacatgatac agggcagttt tctcaattaa atagtaaatt atcctcagaa gagaaaagat
    15061 tattttctac tattctaatg aatagtggta atatggaaat ccaagagatg aatacgggtg
    15121 tgcctggaaa taaagttatg aaaaaattac cgctatcttc gttagagctg tcttatgctg
    15181 aaagaatagg ggaccccaaa atatggaata tggtgaaagg gtattcgtca tttgtataag
    15241 aggaatatat ggaagattta gcagatgtta tttgccgagc cttgggtatc ccttcaattg
    15301 ataccgatga tcaagcaatc atgcttgatg atgatgtgtt aatatatatt gaaaaagaag
    15361 gagattcaat aaatttatta tgcccttttt gtgcattgcc tgaaaatata aatgatctta
    15421 tatatgcgtt gagtctaaat tactcagaaa agatatgttt agctactgat gatgaaggtg
    15481 gaagtctaat tgcgcgatta gatcttaccg ggataaatga attcgaggat atatatgtta
    15541 atacagaata ttatatttcg cgtgtgcgtt ggctgaagga tgaatttgcg cgaagaatga
    15601 aggggtatta atgtcccgtt tcgtatttat tttgctgttt tttatcccat atttagggag
    15661 agctgattgt tgggataagg ctggtgaaag gtacaacatt ccatcgagtt tgttaaaagc
    15721 gattgcggaa aaagaatcca gatttaataa atctgcagtc aatgttaata acaatggaag
    15781 taaagattat ggtataatgc aaataaatga ctttcattct aaaagactta gagaaatggg
    15841 atatactgag gaaatgctaa ttagtcatcc atgtctttct gtacattatg cagcaaagtt
    15901 attaaatgag tttatgctga tgtatggaag agggtgggag gctgttggtg cctataatgc
    15961 aggaacatct ccgaaaaaga aaaaagaaag attaaagtac gcagaggata tctataaacg
    16021 ctatttgcgg atagcagcag agagcaagca gaataatcga aggatataag aggatttatg
    16081 tctgaggcaa agaactcaaa tcttgcacca ttcaggttat tagtgaaact cacaaacggt
    16141 gtcggggatg agtttccgtt atattatggg aataacttaa ttgttctcgg acggacgata
    16201 gaaacacttg agtttggaaa cgataatttc ccagaaaata ttattccagt aacagatagt
    16261 aaatcggatg gtataattta tttggccata agcaaagata atatctgtca atttagtgat
    16321 gaaaaaggtg aacaaattga tatcaattca caatttaact catttgaata tgacggagtt
    16381 tcttttcaac ttaaaaatat gatggaggat aaatcgagag ttcatatact aaacggaatg
    16441 tataataaac cctcggtgtt ttttttcttt gctgttattg ttgtgttaat tataattttt
    16501 tccctctctt taaagaaaga tgaagtaaaa gaaattgctg aaattataga tgataagaga
    16561 tatggaatag tcaacacagg acagtgcaat tatattcttg ccgaaacaca aaatgatgcg
    16621 gtatgggcat ctgtagcctt aaataaaacc ggatttacca aatgcagata tattctggtg
    16681 tccaacaaag aaataaatag aattcaacaa tatatcaatc aacgattccc atatatcaat
    16741 ttgtatgtgt tgaatttagt gagtgataaa gcggaattac tggtattttt aagtaaagaa
    16801 agaaatagtt caaaagatac cgaacttgag aaattgaaaa atgctctgat tgttgaattt
    16861 ccgtatatca aaaatattaa attcaactat cttagcgatc ataatgcaag ggaggatgct
    16921 aaaggtattt tcacaaaggt aaatgtccag tataaagaga tttgtgaagc taataaagtt
    16981 acatattctg tcagagaaga actgacagat gaaaagcttg agctcattaa tagactgatt
    17041 tctgatcata aaaatgtata tggagatcag tatattgagt tttctgtact tttgatagat
    17101 gatgatttta aaggtaaatc atatcttaac agcaaagaca gttatgtaat gttgaatgat
    17161 aaacactggt tttttttaga taaaaataag tgaggataaa atgagtgtta cagtaccgga
    17221 taaagattgg actctgagtt cattatctga aacttttgat gatggaactc aaacattaca
    17281 aggtgaacta acattggcac tagatgcatt agctaaaaat ccttcgaatc cacagttgct
    17341 ggctgaatac caaagtaaat tatctgaata tacattatat aggaacgcgc aatctaatac
    17401 tgtgaaagtg attaaggatg ttgatgctgc aattattcaa aacttcagat aatagggagc
    17461 attcatgaat tacatttatc cagtcaatca ggttgatatt atcaaagcca gtgattttca
    17521 atctcaagag atatcaagtc tggaagacgt cgtgtcggct aaatatagtg atattaagat
    17581 ggatacagat attcaagtat cacaaataat ggagatggta ggcaatccag aatcattaaa
    17641 tccagaatct ttggccaagt tacagacgac gctctcaaat tattcaatag gagtatcatt
    17701 agctggcacg ttagcaagaa aaacagtttc ggctgttgaa actttattaa agtcttaatt
    17761 tatatgatta ggtataaagg ttttatttta ttcttgttgc tgatgttgat tggatgtgag
    17821 caacgtgaag agttaatttc taatttatct caaagacagg caaatgaaat aatatctgtg
    17881 ctagaacgcc ataatattac tgctagaaaa gttgatggag gtaaacaggg gatctcggta
    17941 caagtcgaaa aggggacatt tgcatcggca gttgatttga tgcgcatgta cgatttgcca
    18001 aatccggaga gagttgatat ctcacaaatg tttcctacag attcattagt gtcttctcca
    18061 agagctgaaa aggcccgttt atatagtgct attgagcaac ggctggaaca gtctttagtt
    18121 tctattggtg gtgttatttc ggcaaaaata catgttagct atgatcttga agaaaaaaat
    18181 atatcttcaa aaccgatgca tatatcagta atcgctatat atgactcacc gaaagagtct
    18241 gaactattag ttagtaatat taagcgattt ttgaaaaaca ccttttctga tgttaagtat
    18301 gaaaatatat ctgtcatatt aactccgaaa gaagaatatg tttatacaaa tgtacaacct
    18361 gttaaggaaa ttaaatcgga atttttaaca aatgaagtaa tatatgtatt tctcgggatg
    18421 gctgtactag ttgtcactct tttggtatgg gcattcaaaa cagggtggtt caagagaaac
    18481 aaaatatgat aagaatggat ggaatttata aaaaatatct ttcaataatt tttgatccag
    18541 cgttctatat aaatagaaat cggttgaatt tgccttctga actgttagaa aatggcgtaa
    18601 tcagaagtga gattaataat ctcataatta ataaatatga tctaaattgc gatattgaac
    18661 ctttaagcgg ggtaaccgct atgtttgttg ccaactggaa tttacttcca gctgttgctt
    18721 attttatcgg tagtcaagag tctcggttaa ttaatcatag tgagatggtt attagttatt
    18781 atgggggcaa aatcagtaag caaggggagg ccgctattcg aagtggtttt tggcatctta
    18841 ttgcgtggaa ggaaaatata tctgttggta tatatgaaag aataaactta ttatttaatc
    18901 caattgcttt ggagggaaat tatacgcctg tagaaagaaa tttgtctcga ttaaatgaag
    18961 gtatgcaata tgcaaaaagg cactttaccg gtatccagac atcatgccta tgatggtgtt
    19021 gttataaaga gaattgaaaa ggaattatgt aaaaccataa aagatagaga cactgagagt
    19081 aagaagaaag caatttgtgt tataaaggat gctacgaaaa aagctgaatc actgcgtata
    19141 gatgctgtat gtgatggtta tcaaattggt atacagactg cttttgagca tattattgac
    19201 tatatttgtg aatggaagtt aaagcaaaat gaaaacagaa gaaatattga ggactatata
    19261 actagtttgc tttcagaaaa tttacatgat gagaggatca ttaacacttt attggagcag
    19321 tggcttagtt cgttgcgtaa tacagtaaca gaattaaaag tggttttgcc taagtgtaat
    19381 ttagcactga ggaaaaagtt agaacttgat ctccataaat atcgctcaga tgtcaagatt
    19441 attttaaaat attctgaagg gaataattat atattctgta gtggtaatca agtagtggag
    19501 tttagtcctc aggatgtaat atcaggtgtt aaaatagaac ttgcggaaaa attaacaaaa
    19561 aatgataaaa aatatttcaa agagttagca cataagaaac taagacaaat tgcagaagac
    19621 ttgttaaaag agaatcctgt taatgattaa tcaaataaat gcaagcaatg ctctgcagca
    19681 aagattaaat agtgaagaat ttgttaattt aaatgagaga ttatcatctt cgcaatcatt
    19741 tgatgaggat ataatctatg aaataatgca atatttctca cagagtgaat taaattccat
    19801 agataatgat gaactgcaca ataagattga acaattattc aattcacgtt ttccttatct
    19861 tacagctgca caaaaaagtt cacttcttaa taaactcata gatgcaaatc aatatgtcga
    19921 tttgcatgag ggattttatg catcattgtc catatataac aatattgatt tctatattaa
    19981 gactacaacc ttcgattcat taattagtgt ctttgacgca gggagagagg cagatgattc
    20041 gacatggtag taataagttg aaaatattta ttttaagtat attgctatta acactgagtg
    20101 ggtgtgcttt aaagtcatca tctaattctg aaaaagaatg gcatattgtt cctgtaagta
    20161 aggattattt ttctattcca aatgatttat tatggtcgtt taatacaacc aataaaagta
    20221 taaatgttta ctctaaatgt attagtggta aggcggttta tagttttaat gcaggtaaat
    20281 tcatgggcaa ctttaatgtt aaggaagtag atgggtgctt catggatgca caaaagatag
    20341 ctatagataa actattttct atgctgaaag acggggttgt tttaaaaggt aataagataa
    20401 atgataccat ccttatagag aaggatgggg aagttaaatt aaaattaatt cgagggatat
    20461 aattgtattg tgagtaaata taaaggtcta aatacaagta atatgtttta catttactct
    20521 agtggacatg aaccagttaa cgttgagctt gtaaaagata aagaacgtaa cataattgag
    20581 ctggctccag catggaaggg tttttttttg tccgaaatca aaatataaaa ttcagtgata
    20641 atgtgaacta tcattatcgc tttaacatta atagttgtgc aaaatttctt gctttttggg
    20701 actatttcag tggtgctcta gtagagcata gccatgctga aaaatgtatt catttctatc
    20761 atgagaatga tcttcgggat agttgtaata ccgaatcgat gcttgataag ttaatgttaa
    20821 gattcatctt ttcatctgac cagaatgtaa gtaatgcact ggctatgata cgaatgactg
    20881 agtcatatca tttggttctt tatcttctga gaactattga gaaagaaaag gaggtaagaa
    20941 taaagagtct gacagagcat tatggtgtgt cggaggctta ttttagatct ttatgtagaa
    21001 aagctttagg ggcaaaagtc aaagaacaac tgaacacttg gcggttagta aatggtttat
    21061 tagatgtatt tttgcacaat caaacaatca cttctgctgc catgaataat gggtatgcat
    21121 caacatccca tttttcaaat gagataaaaa cacgactagg cttttctgca agagaattat
    21181 cgaatattac ttttttagtg aaaaaaataa atgaaaaaat ttaatattaa atctttgact
    21241 ctcttgattg tattgttacc attgatagtt aatgctaata atattgactc tcatttatta
    21301 gaacaaaatg acatagcgaa atatgttgct caaagtgata cggtaggatc tttttttgaa
    21361 cgtttttctg cgttattaaa ttatcctatt gttgttagca aacaagctgc aaaaaagaga
    21421 atatctgggg agtttgattt atctaatcca gaagaaatgc ttgaaaaact gacattactt
    21481 gtgggactca tatggtataa agatggaaat gcattataca tatatgactc tggagaatta
    21541 ataagcaaag ttattttgct ggagaatata tcacttaatt atcttattca gtatctgaag
    21601 gatgctaacc tttatgatca cagatatcct ataagaggaa acattagtga taaaacattc
    21661 tatatatctg gtcctccggc attggttgaa ctggtagcta atacagcaac tttgcttgat
    21721 aagcaggttt cttctattgg gacagataaa gttaattttg gggttataaa attaaaaaat
    21781 acatttgtta gtgacagaac ttataatatg agaggggagg atattgtcat cccaggggtg
    21841 gctactgttg tagaacgttt attaaataat ggtaaggctc tgagtaacag acaagccaaa
    21901 aatgatccga tgccaccctt taatattaca cagaaagtat ctgaggatag taatgacttt
    21961 tcatttagtt cagttaccaa tagttctata ttagaggatg tatcacttat tgcatatcca
    22021 gaaaccaatt ctatattagt caaaggtaat gatcagcaaa tacagattat tcgcgatatt
    22081 atcactcagt tagatgtagc caaacgacat atagaattat cattatggat aatcgatatt
    22141 gataaatctg aattaaataa ccttggcgtt aattggcaag gaacggcttc atttggtgat
    22201 tcgtttggtg cgtcatttaa tatgagctca tcagcatcga taagcacttt ggacggcaat
    22261 aagtttattg cttcagttat ggcattaaat cagaaaaaga aagcaaatgt agtttcaagg
    22321 ccggtgattt tgacacaaga gaatattccg gctatatttg ataataacag gactttttat
    22381 gtgtcattag ttggtgagcg caattcttcg ttggagcatg ttacgtatgg aacattgata
    22441 aatgttatac ctcgcttttc atctcgggga caaattgaga tgtctttaac gatagaggat
    22501 ggaacaggaa atagtcagtc aaattacaat tataataatg aaaatacatc tgtccttcca
    22561 gaagttggaa ggaccaaaat cagtacaata gcgagagttc cacaaggcaa aagtttgttg
    22621 ataggtggtt atacgcatga aacgaactct aacgaaatag tatctattcc ttttctctcc
    22681 tctattcctg taatcggtaa tgtgttcaag tataaaacaa gtaatatcag taatattgta
    22741 agggtttttc tgattcaacc ccgtgagata aaggagtcaa gttattataa tactgcagaa
    22801 tataagagcc tcatatctga aagagaaata caaaagacta cacaaataat cccatctgaa
    22861 actactttgc tggaagacga aaaatcattg gtttcatact taaattacta actataaagt
    22921 aggtgatgta tgcttgatgt taaaaataca ggagttttta gctctgcatt cattgataag
    22981 ttgaatgcaa tgacaaattc agatgatgga gatgagactg ctgatgcaga gcttgattct
    23041 ggcttggcta atagcaagta tattgactca tctgatgaga tggcttccgc tctttcgtca
    23101 tttataaaca gaagagacct tgagaaactg aaaggaacaa atagtgatag tcaggaacgt
    23161 attttagatg gggaagaaga tgaaattaat cacaagattt ttgatttaaa gagaacttta
    23221 aaagataacc ttcctctaga tcaggatttt atagacagac taaagagata ttttaaagat
    23281 ccaagtgatc aagtcttagc attaagggaa cttttgaatg aaaaagatct tactgctgaa
    23341 caagtcgaat cattaactaa aattattaat gagataatat caggtagtga aaaaagtgtt
    23401 aatgctggaa taaattcagc tatacaggct aaattatttg gcaacaaaat gaaacttgaa
    23461 ccacagcttt tgcgtgcatg ttatcgtggt tttatcatgg ggaacatatc aacaacagat
    23521 cagtatatag aatggcttgg taattttggt tttaatcaca gacatacaat tgtgaatttt
    23581 gtagagcagt cactgattgt agacatggat tctgagaaac cgagctgtaa tgcttatgag
    23641 tttggttttg tgttatctaa attaattgca attaagatga ttagaacttc agatgtaatt
    23701 tttatgaaga aactggaatc ctcaagcttg ctaaaagatg gcagtttaag tgcagagcag
    23761 ctattgctaa ctttattata tatttttcaa tatccaagtg aaagtgagca aattcttact
    23821 tctgttatag acgtatcacg agccagtcat gaggattctg tagtgtatca aacatatcta
    23881 tcttctgtta atgaaagtcc tcatgatata tttaaaagtg aaagtgaaag agaaattgcg
    23941 atcaatattc tacgagagct tgtcacaagt gcatacaaga aagagctttc tagataacag
    24001 gagatgaaag tgatccagtc ttttcttaag caagtaagta ctaagcctga attaattata
    24061 ttagtgctta tggttatgat cattgctatg ttgatcatac cattacctac ataccttgtt
    24121 gattttttaa tagggcttaa tattgtactt gctattctcg tttttatggg atcattttat
    24181 attgaaagaa tattgagttt ttctacattc ccttcggttt tgttgataac gacattattt
    24241 cgtctcgctt tatcaataag tactagtcga ctaattttag ttgatgcgga tgcgggtaaa
    24301 attattacta catttggcca gtttgtcatt ggtgatagtc tggctgtagg ttttgttatt
    24361 ttttcaatag taactgttgt gcagttcatt gttataacaa aaggatctga aagggttgcg
    24421 gaggtagctg ctcgcttttc tcttgacggt atgccgggga aacaaatgag cattgatgcg
    24481 gatttaaagg ctggaattat tgatgccgca ggagctaaag aaagacgcag tattctggaa
    24541 cgtgagagtc aattgtatgg ttcatttgat ggagcaatga agttcattaa aggtgatgca
    24601 attgctggca tcattattat ttttgtcaat ttaataggag gtatttctgt tggtatgagt
    24661 caacatggaa tgtctctatc tggtgcatta tctacttata ccatactaac tattggtgac
    24721 ggattggtct ctcagattcc tgctctgtta atttctataa gtgcaggatt tatcgttaca
    24781 agagttaatg gtgatagcga taatatggga cgtaacataa tgtcccagat atttgggaat
    24841 ccttttgttc tcattgttac atcagctctt gccttggcga tagggatgtt gccaggtttt
    24901 ccgttttttg tttttttcct gatagcagtt actttgacgg ctttattcta ttataaaaag
    24961 gtcgtagaaa aagaaaaaag tctgtctgag tctgattcta gtggctatac tggtacattt
    25021 gatattgata atagccatga ctcatctttg gcaatgatag aaaatctgga tgctattagt
    25081 tcagaaaccg ttcctttaat tttattattt gccgaaaata agataagtgc aaatgatatg
    25141 gaaggtctta ttgaaaggat aagaagtcag ttctttattg attatggtgt caggcttcca
    25201 actattttat atagaacaag caatgagctt aaggtcgatg atattgtttt gttaataaat
    25261 gaggtgcgtg ccgatagttt taatatatac tttgacaaag tgtgtattac agatgaaaat
    25321 ggagatatag atgctctagg tattcctgtt gtttcaactt catataatga acgtgttatt
    25381 tcttgggtag acgtttcata tacagaaaat ctaactaata ttgatgctaa aattaaaagt
    25441 gctcaagatg aattttatca ccagttgtca caagctttat taaacaacat aaatgagatt
    25501 tttggtatac aagaaacaaa aaatatgtta gatcagtttg aaaatcggta tcctgatcta
    25561 ttaaaggaag tcttccgaca tgtgactata cagagaattt ctgaggtatt acaaagattg
    25621 cttggagaaa atatttctgt tcgcaattta aaacttatta tggagtcttt ggcgctttgg
    25681 gctcccagag aaaaagatgt tataacatta gttgaacatg tccgtgcatc actttctagg
    25741 tatatttgta gtaaaatagc tgtttctggt gagattaaag ttgtgatgct ttccggatat
    25801 attgaggatg caataagaaa agggataagg caaacctctg gtggctcttt cttgaatatg
    25861 gatatagagg tttcggatga ggtaatggaa actttagcac atgctttgag agaattgaga
    25921 aatgcaaaaa aaaatttcgt tcttttggta tcagtagata tacgtaggtt tgttaaaaga
    25981 cttatagata acagatttaa gagtatactc gttatatcgt atgctgagat tgatgaagca
    26041 tataccatta atgtattaaa gactatttag tgaggtttaa atatgagtaa cattaattta
    26101 gttcaattag ttagagatag tcttttcacg attggttgtc ctccatcaat tattacagat
    26161 ttagacagcc attcagcgat taccatttct ttggatagta tgcctgctat taatatagct
    26221 cttgtcaatg agcaggttat gttatgggct aattttgatg caccaagtga tgtaaaactt
    26281 cagtcatctg cttataacat attaaattta atgttaatga actttagtta tagcattaat
    26341 gagctggtag agcttcatcg ttctgatgag tacttacagt taagagttgt tattaaagat
    26401 gactacgttc acgatgggat tgtttttgca gagattctgc atgagtttta tcagaggatg
    26461 gaaatcttaa atgaggtctt ataatcaatg agctatataa aattgctcac tcaattatct
    26521 tttcctaata gaatctcggg gccaatcttg gaaacaagtc ttagcgatgt ttcgattggt
    26581 gagatttgta acattcaggc tggaattgaa agtaatgaaa ttattgcaag agctcaggtt
    26641 gtaggatttc atgatgaaaa aacaatatta agcttgattg gaaattctcg tggactttca
    26701 cggcaaacgt tgattaagcc cactgcccag tttcttcata cgcaagtcgg ccgtggatta
    26761 ttgggagcag tagtcaatcc tttaggggag gttactgata agtttgctgt tacggataac
    26821 agtgaaattc tttatcgacc tgtagataat gctcctccgc tatatagtga aagggctgca
    26881 attgagaagc cttttttaac aggtgttaag gttattgatt ctttactcac gtgtggtgaa
    26941 ggacagcgaa tggggatttt tgcgtcagct ggttgtggca aaacttttct catgaatatg
    27001 ctcattgaac atagtggtgc tgatatatat gttattgggt taattggtga gcgaggtcga
    27061 gaggttacag aaacggttga ttatttgaaa aactctgaga aaaaaagcag gtgtgtttta
    27121 gtatatgcaa cttcggatta ctcttcggtt gatcgttgta atgctgcata tatagccact
    27181 gctatagccg aattttttag gactgaagga cataaagtag cgctttttat tgattcatta
    27241 acaaggtatg ccagagcatt acgtgatgtg gccttagcag ctggagaatc acctgccaga
    27301 agaggctatc cggtttcggt ttttgatagc ttacccagac ttcttgaaag gccaggaaag
    27361 ttaaaggcag gtggctctat tactgcattt tacactgttc ttttggagga tgatgatttt
    27421 gctgatccat tagctgaaga ggtaagatcc attttagatg gacatatata tttgagcaga
    27481 aatctagccc aaaaaggaca atttcctgca attgattcct taaaaagtat aagcagggta
    27541 tttacacagg ttgttgatga aaaacatcgt attatggccg ctgcatttcg ggagctactt
    27601 tctgaaatag aagagctaag gacaattatt gactttggtg aatacaaacc gggggagaat
    27661 gcctctcagg ataaaatcta caacaaaata tctgttgttg agagttttct gaagcaagat
    27721 tatcgactcg gttttactta tgagcagaca atggagctta ttggtgaaac aattagataa
    27781 ggttttaaaa atcaaagata aatatcaacg gtctgttaaa ttaattgaag cacacatatt
    27841 aacactaaaa aaaaaactct ttgtatcgag atgtggaggc attagataaa aggattatat
    27901 acttccagca actggagaat gacttggaac ctgtaggcgc tcagagtgtt agtcagcttt
    27961 tcaatacaag acgtaagatc gctatagtaa aaaagcatat tatccaatat cagtctgaac
    28021 gaattcttct aaagggaaga attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt
    28081 ctaaaagaaa actgcttcat aaagagagta aaatatgtaa acggattggc ttgattaaaa
    28141 gaaataactt tgccaaacag ttgatacttg atgagttatc acaagaggat atgaagtatg
    28201 gcattagata atataaacct aaattttagt agtgacaaac agatagaaaa atgtgagaaa
    28261 ctatcttcaa tagataatat tgatagtctc gttttgaaga agaagaggaa ggttgaaatt
    28321 cctgagtact ctttaattgc atctaactat tttactattg ataagcactt tgaacataag
    28381 catgataaag gagaaattta tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga
    28441 gcgacatatt ctgatattcc agaatcaatg gccattaaag aaaatatttt gataccagat
    28501 caagatatca aagcaaggga aaaaataaat atcggcgata tgagggggat cttttcatat
    28561 aataagagtg gaaatgcaga caagaacttc gaaagaagtc atacttcttc tgtaaaccct
    28621 gataatctgc tagaatctga taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg
    28681 tctattgata agaatattgc tgacatcatt tctttactaa atggaagtgt tgctaaatca
    28741 tttgagctgc ctgtaatgaa taaaaatact gcagacataa ccccatccat gtcattgcaa
    28801 gaaaaatcaa tagttgaaaa tgataaaaat gtttttcaaa aaaatagtga aatgacttac
    28861 cactttaaac agtggggggc tggacattct gttagtattt cagtggagtc tggttctttt
    28921 gttctaaaac cgtcagatca atttgtagga aataaacttg acttaatttt gaaacaagat
    28981 gctgagggta attacagatt tgatagcagt caacataata aggggaataa aaataatagt
    29041 acaggatata atgaacagag tgaagaagaa tgctaagaat taaacatttt gacgctaacg
    29101 aaaaactaca gattttatat gcaaagcaac tctgcgagcg tttttcgatt cagacattca
    29161 aaaataaatt tacaggcagt gaaagtttag tcactcttac ttccgtgtgt ggggattggg
    29221 taattcgtat tgatacatta tcttttttga aaaaaaaata cgaggtattt tcaggatttt
    29281 ctacacaaga atctttactg catttatcaa aatgtgtctt tatagagtcg tcatctgtat
    29341 tttcgattcc agaactgtct gataagatta ctttccggat cacgaatgaa atccagtatg
    29401 caactactgg gagtcattta tgctgttttt cctcttcttt aggtattatt tattttgaca
    29461 agatgccggt attacgtaat caagtttctc ttgacttatt gcatcatctt ttagagtttt
    29521 gcttaggttc atctaatgta aggctggcta ctttaaaaag aattcgtact ggtgatataa
    29581 tcatagttca gaaactttat aatttattat tgtgtaatca agttattatt ggggattata
    29641 ttgtgaatga taataatgag gcaaaaatta atctgtcaga aagtaatggt gagtcagatc
    29701 acacagaagt ttctttggca ttattcaatt atgatgatat caatgtaaaa gtggacttta
    29761 tccttttaga aaaaaagatg acaatcaatg aactaaaaat gtatgtagaa aacgaattat
    29821 tcaagtttcc cgatgacata gttaaacatg taaatattaa agtaaatggt tctttggttg
    29881 ggcatgggga acttgtttct attgaggatg gttatggtat cgagattagt tcttggatgg
    29941 taaaggagta atctcatgct gagtgatatg tccctcatcg ctacactttc tttttttacg
    30001 ctattaccat ttttagttgc tgctggtact tgttatataa agttttctat tgtttttgta
    30061 atggtacgaa atgcacttgg tttgcagcag gttccatcaa atatgactct caatggtatt
    30121 gcattgatta tggctttgtt cgtgatgaaa cccattattg aagctggtta tgaaaattat
    30181 ctaaatggcc cgcaaaaatt tgatacaata tcagatattg tccgtttttc tgattctggc
    30241 ttgattgaat acaagcaata cttgaaaaag catacagatc ttgagttggc acgttttttc
    30301 caaaggtctg aagaagaaaa tgctgattta aagagtgcag agaataatga ttattcattg
    30361 ttttccctct tgcctgcata tgcgctaagt gaaattaaag atgcgttcaa aataggtttt
    30421 tatttatatt tgccatttat tgttgttgat cttgttattt caagcatttt acttgcactt
    30481 ggtatgatga tgatgagtcc aatcacaata tcagtaccaa taaaacttgt attatttgtt
    30541 gcgcttgatg ggtgggggat cctgtctaag gccttgattg aacaatatat caatgttcct
    30601 gcttagatga tggaggtaat gtgtctgata tagtttatat gggtaataag gctctttatt
    30661 taatccttat cttttcctta tggccagtag gtatagctac ggttattgga ttaactattg
    30721 gtttattaca gacagtgact caacttcaag agcagacact tccttttggt ataaagctta
    30781 taggtgtctc aatatctttg ctacttcttt ctggatggta tggtgaggtt ttattgtctt
    30841 tttgtcatga aataatgttt ttaattaaga gtggggtttg atggacattt caagctggtt
    30901 cgaaagtatt catgtgtttt taatactcct gaacggcgtt ttttttagat tggctccatt
    30961 atttttcttt cttccatttt taaataacgg tataatttct ccatctatta gaatacctgt
    31021 gatttttctt gttgcatcag gattaattac ttctggtaag gtagacatag gttcttctgt
    31081 ttttgaacat gtttatttcc ttatgttcaa ggaaataatt gttggcctcc ttctctcttt
    31141 ttgcttgtct cttccctttt ggatatttca tgctgttggt agcattattg acaaccagcg
    31201 tggggcaacg cttagtagtt caattgatcc tgccaatggt gttgatacgt ctgagttggc
    31261 aaaatttttc aatctttttt ctgcagttgt atttctatac agtggtggaa tggtctttat
    31321 tttagaatcc atacaattgt cttataatat atgcccgtta ttttctcaat gttctttccg
    31381 tgtctcaaat atcttaacat ttctgacttt attggcaagt caggctgtta ttttagccag
    31441 tcctgttatg atagtattgt tactatcaga agtattactt ggtgtattat cgagatttgc
    31501 tccgcagatg aatgcttttt ccgtatcatt aactattaaa agtttacttg caatatttat
    31561 tattttcatc tgttcttcta ctatttactt ttctaaagtt caatttttcc tcggtgaaca
    31621 taaatttttc acaaatctat ttgttagata aaatattatg gcaaataaaa cagaaaagcc
    31681 gacacctaaa aaactaaagg atgccgcaaa aaaaggacag tcatttaaat ttaaggattt
    31741 aacgactgtt gttattattc tggtagggac atttactata atatcattct tttccttaag
    31801 tgatgtaatg ctcttataca gatatgtaat aattaatgac ttcgaaatta atgagggtaa
    31861 atactttttt gcagtggtta ttgtcttttt taagataatt ggcttcccac tttttttctg
    31921 tgttctttcg gctgtgttgc caacattggt tcaaacaaag tttgttcttg cgactaaagc
    31981 tatcaagatt gatttttcag tattaaaccc tgttaaaggg ttaaaaaaaa tatttagtat
    32041 aaagacaata aaagaatttt tcaaaagcat tctgcttctt attattctag cattaacaac
    32101 ctatttcttt tggattaatg accgaaaaat aattttttct caggtgtttt ctagtgttga
    32161 tggcttatat cttatttggg gggggctgtt taaggatata atattatttt tcttggcatt
    32221 ttctattttt gttattattc ttgactttgt gattgagttc attttataca tgaaagatat
    32281 gatgatggat aaacaggaga taaaaagaga atatatagag caagagggac actttgagac
    32341 aaagtcgaga aggcgtgagt tgcatatcga gattctttca gagcagacta aatctgatat
    32401 acgtaattca aaattagtgg taatgaaccc gactcatatt gcaattggta tttattttaa
    32461 tccagaaata gcgcctgcac cttttatttc tctcattgaa actaaccagt gtgccttggc
    32521 tgtcagaaaa tatgcaaatg aagttggtat accaactgtg cgtgatgtga aattagctag
    32581 aaaactatat aaaacacata caaaatatag ttttgttgat tttgaacact tggatgaagt
    32641 cctacgtctt attgtttggc ttgagcaggt tgaaaacact cattaataag taaaggagat
    32701 gtgttatatg ggagttaatt tctgtaataa aataggtatt gatcagtctg aatttgaaat
    32761 agaatcttcc atcattaact ccattgctaa tgaggtattg aacccaatat ctttccttag
    32821 caacaaggat ataataaatg ttttactcag aaagatttca tctgaatgtg acctagttag
    32881 aaaagacatt tatcgttgtg ctctggagtt ggtcgttgaa aaaacacctg atgatttata
    32941 atcattcata ataactcttt tgctgatata aatatttttg ttgaagatat ttatgtagtc
    33001 acaggatcaa ttgtagaaaa ttgttatgtt tttgttagga actgcactaa ataatatatg
    33061 ataagacagc aaaaacgact gaccataatc cttcttttat taggagttga taagagagac
    33121 tattcatcct gtaatgttaa aactctgtta tatagtattc gcgattatgc aaagtctgta
    33181 aatgatcatg agatactaac cgagagtaat cgtttattat ctcattgcat ttcagatagt
    33241 aacggagctt tctttaaaag tagtaaatac gttcccctta agtacttacg taaaaggaga
    33301 atagcacgaa agataccaaa tgattagcac tttgctagta agcttgctgg taaactatat
    33361 tgaccgttgg gtttgttttg agactgtaat ttaaattgtg taattgcctg tttttgatat
    33421 gtttactcca ac
//
LOCUS       DADLSB010000028        33214 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.028, whole genome shotgun sequence.
ACCESSION   DADLSB010000028 DADLSB010000000
VERSION     DADLSB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.028"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..559
                     /gene="mdfA"
                     /locus_tag="JN331_001314"
     CDS             <1..559
                     /gene="mdfA"
                     /locus_tag="JN331_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY7259504.1"
                     /translation="LALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALI
                     AGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGI
                     GLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNL
                     VNGILWLSLMVIFLKDKQMGNSHEG"
     gene            complement(600..884)
                     /locus_tag="JN331_001315"
     CDS             complement(600..884)
                     /locus_tag="JN331_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259505.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(970..1785)
                     /gene="ybjI"
                     /locus_tag="JN331_001316"
     CDS             complement(970..1785)
                     /gene="ybjI"
                     /locus_tag="JN331_001316"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY7259506.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(1785..2993)
                     /locus_tag="JN331_001317"
                     /pseudo
     CDS             complement(1785..2993)
                     /locus_tag="JN331_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3077..3613
                     /locus_tag="JN331_001318"
     CDS             3077..3613
                     /locus_tag="JN331_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7259507.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(3788..5473)
                     /locus_tag="JN331_001319"
     CDS             complement(3788..5473)
                     /locus_tag="JN331_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY7259508.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            5743..6120
                     /locus_tag="JN331_001320"
     CDS             5743..6120
                     /locus_tag="JN331_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259509.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(6150..6407)
                     /gene="grxA"
                     /locus_tag="JN331_001321"
     CDS             complement(6150..6407)
                     /gene="grxA"
                     /locus_tag="JN331_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY7259510.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            6567..6854
                     /locus_tag="JN331_001322"
     CDS             6567..6854
                     /locus_tag="JN331_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY7259511.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            6838..7560
                     /gene="nfsA"
                     /locus_tag="JN331_001323"
     CDS             6838..7560
                     /gene="nfsA"
                     /locus_tag="JN331_001323"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY7259512.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            7621..8523
                     /gene="rimK"
                     /locus_tag="JN331_001324"
     CDS             7621..8523
                     /gene="rimK"
                     /locus_tag="JN331_001324"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY7259513.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERWAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            8611..9087
                     /locus_tag="JN331_001325"
     CDS             8611..9087
                     /locus_tag="JN331_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY7259514.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            9439..10551
                     /gene="potF"
                     /locus_tag="JN331_001326"
     CDS             9439..10551
                     /gene="potF"
                     /locus_tag="JN331_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY7259515.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            10646..11779
                     /gene="potG"
                     /locus_tag="JN331_001327"
     CDS             10646..11779
                     /gene="potG"
                     /locus_tag="JN331_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY7259516.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            11789..12742
                     /gene="potH"
                     /locus_tag="JN331_001328"
     CDS             11789..12742
                     /gene="potH"
                     /locus_tag="JN331_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY7259517.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            12739..13584
                     /gene="potI"
                     /locus_tag="JN331_001329"
     CDS             12739..13584
                     /gene="potI"
                     /locus_tag="JN331_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY7259518.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            13644..14132
                     /locus_tag="JN331_001330"
     CDS             13644..14132
                     /locus_tag="JN331_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY7259519.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            14173..15300
                     /gene="rlmC"
                     /locus_tag="JN331_001331"
     CDS             14173..15300
                     /gene="rlmC"
                     /locus_tag="JN331_001331"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY7259520.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(15475..16206)
                     /gene="artJ"
                     /locus_tag="JN331_001332"
     CDS             complement(15475..16206)
                     /gene="artJ"
                     /locus_tag="JN331_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7259521.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(16498..17166)
                     /gene="artM"
                     /locus_tag="JN331_001333"
     CDS             complement(16498..17166)
                     /gene="artM"
                     /locus_tag="JN331_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY7259522.1"
                     /translation="MFEYLPELMKGLHTSLTLTFASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTQAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(17166..17882)
                     /gene="artQ"
                     /locus_tag="JN331_001334"
     CDS             complement(17166..17882)
                     /gene="artQ"
                     /locus_tag="JN331_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY7259523.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSYGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(17889..18620)
                     /gene="artJ"
                     /locus_tag="JN331_001335"
     CDS             complement(17889..18620)
                     /gene="artJ"
                     /locus_tag="JN331_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7259524.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(18638..19366)
                     /gene="artP"
                     /locus_tag="JN331_001336"
     CDS             complement(18638..19366)
                     /gene="artP"
                     /locus_tag="JN331_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY7259525.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCSVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(19584..20099)
                     /locus_tag="JN331_001337"
     CDS             complement(19584..20099)
                     /locus_tag="JN331_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7259526.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            20748..22517
                     /locus_tag="JN331_001338"
     CDS             20748..22517
                     /locus_tag="JN331_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7259527.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            22728..23051
                     /locus_tag="JN331_001339"
     CDS             22728..23051
                     /locus_tag="JN331_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY7259528.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            23048..23878
                     /locus_tag="JN331_001340"
                     /pseudo
     CDS             23048..23878
                     /locus_tag="JN331_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(23875..24888)
                     /locus_tag="JN331_001341"
     CDS             complement(23875..24888)
                     /locus_tag="JN331_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7259529.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(24987..26417)
                     /locus_tag="JN331_001342"
     CDS             complement(24987..26417)
                     /locus_tag="JN331_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7259530.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(26428..27429)
                     /gene="ltaE"
                     /locus_tag="JN331_001343"
     CDS             complement(26428..27429)
                     /gene="ltaE"
                     /locus_tag="JN331_001343"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY7259531.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSRILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(27466..29184)
                     /gene="poxB"
                     /locus_tag="JN331_001344"
     CDS             complement(27466..29184)
                     /gene="poxB"
                     /locus_tag="JN331_001344"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY7259532.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTKFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(29317..30285)
                     /gene="hcr"
                     /locus_tag="JN331_001345"
     CDS             complement(29317..30285)
                     /gene="hcr"
                     /locus_tag="JN331_001345"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY7259533.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(30297..31949)
                     /gene="hcp"
                     /locus_tag="JN331_001346"
                     /gene_synonym="priS"
     CDS             complement(30297..31949)
                     /gene="hcp"
                     /locus_tag="JN331_001346"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY7259534.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(32093..32992)
                     /gene="lysO"
                     /locus_tag="JN331_001347"
     CDS             complement(32093..32992)
                     /gene="lysO"
                     /locus_tag="JN331_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY7259535.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8258 a         8228 c         8529 g         8199 t
ORIGIN      
        1 gctggcgctg ggattcgtta gcctgccatt gctggcgtgg atcgcccagt cgccgattat
       61 catcattacc ggggagcagt tgagcagcta tgaatatggc ttgctgcaag tgcctatttt
      121 cggggcgtta attgcgggta acttgctgtt agcgcgtctg acctcgcgcc gcaccgtacg
      181 ttcgctgatt attatgggcg gctggccgat tatgattggt ctgttggtcg ctgctgcggc
      241 aacggttatc tcatcgcacg cgtatttatg gatgaccgcc gggttaagta tttatgcttt
      301 cggtattggt ctggcgaatg cgggactggt gcgattaacc ctgtttgcca gcgatatgag
      361 taaaggtacg gtttctgcgg cgatgggaat gctgcaaatg ctgatcttta ccgtcggtat
      421 tgaaatcagc aaacatgcct ggctgaacgg gggcaacgga ctgtttaatc tcttcaacct
      481 tgtcaacgga attttgtggc tgtcgctgat ggttatcttt ttaaaagata aacagatggg
      541 aaattctcac gaagggtaaa aaaatgcctg attgatttgt gcgatcaggc attctcgaat
      601 taatggtgat ggtcgtcaat ctggtgttcg ataaccatcc cttcacctac gctggcaaga
      661 tggcgaacat aaggatgcgg gcggtaagcc ggagctggcg caggagccac atagacggtt
      721 tgcggcacag tcgcgacgct gaccgcttgt ggaacgctga ctgaatcagg aaccacaacc
      781 actttgtatc cactcggcac atcgacggtg acactttgcg ccatcgccac accagtaaag
      841 cccattaaaa gcgcgcccaa cagtagacaa tttttcataa tcatttcgcc tgaaataatg
      901 cagcgggtta atttccgcga attatgcagc cttcattcag gcattgtgtt gccgggcgtg
      961 ctgccgtttt tattggtcaa atggcgcttc gtgtttaaga actttatcga tcacatccag
     1021 tacgccttca cggttattgg agcctgcccg gtattttgcc gctgcgacga ccgcgctgcc
     1081 ggcattttcc attgcaaaac taaagcctgc ctgacgcagc atctcaatat cgttaccgcc
     1141 atcgccaaag accaccactt cgctgtcgtc tattccccat aatttctgca gttggcgaag
     1201 gccattggct ttatgtacgc cggggataat cagatcgatg ctgccgttgc cggtgtggac
     1261 tggcaccata atatcgccga tggcctcatg taatgctttt tgtacttgtg gaatcagttc
     1321 atcggaaaga ttcaggccaa acttaaagaa aatatcttct aagttgtcaa agttatcgac
     1381 gtattccaga cggtgataat acatttccgc caccgttttc atggcatcgt catatttttt
     1441 gagtgtatag gcgctatttt ttccgcaggc aataatttcc acttccgggc gcgtcagcaa
     1501 atgttccacg acagtagcaa acgcatcctt cgacagctcg ccattaaaaa catctttgcc
     1561 ttcactcact acccagccgc cgttttcagc cacaaaggca atttcattcg caatttcagg
     1621 gaagaaagag atcaactgat aatattgatt cccgctggcg accacaaagc gaatgccttg
     1681 tgctttcatt tgctgatact gagccataaa ccgctcacgg ttataggttt tttgatcgct
     1741 taagaaagta ccatccatgt ctaccgcaat taatttaatg ctcatcaact attctccatc
     1801 gccgtctgcg ttttggtatc gggtttggcg acggctttcg cgacaatagc cgcgagaata
     1861 accagcgcca gtacaaccag cattgcacta cgtaatccat aatgttcgcc gagatagccc
     1921 agcagcggcg gcccgacgag gaaagccaga taaccggtcg ttgctaccac actgacgcgg
     1981 gttggtgcat cggggccggt atcgctggcg gcagaaatgg tcagcgggaa gcccagcgag
     2041 gcacccagtc cccaaagtac aacagacact cctgcgaccc aggcgctatc gacaaaaata
     2101 atcagcccaa tacccaacgc ccccattagt gcactggccc gaaccacggc aacgcgactg
     2161 taacggtcga tgaaccaacc gccggtgaag cgtccaacgg tcatccctag ggtaaaaccg
     2221 gcataaatca gtgagccgga agtggggcta aaaccgtgac catcaaccat taataagggt
     2281 aactagtcgt tggcagaacc ttcggcaaag gccatcgcca gcactacaac accaatcaac
     2341 agcaactgga tatcgcgata aaaaggaatg cctttttcgc catgctgggt gccatcggca
     2401 gcatttttgc ccgtaccgtc agggattgcc tgaatggcga tataaatagg cgcgatacct
     2461 accagcgccg ccaataaaat gtgcaccgtt gccggaacgc caaaggccgt cagtgccatc
     2521 ccgacaccag cgcctgccag cgtgcccagg ctataaaaac cgtgcatcat cggcaaaacc
     2581 gttttattca tttctcgctc aacggcggca ccttcaacgt ttatcgccac ttccgcagaa
     2641 ccaaaacttg ccccaaagac gccgagacca acggcaaaga gcaggggcga tgtcagccag
     2701 agtgccagac ttaatatcat catcccgatc aatgcgcagg acatcgtgac caggatgaca
     2761 ttacgtgtcc caaagcgttt cactaaccac gccgagcaga gaataccgct catcgaaccg
     2821 atcgacagac caaagagaac accgcccatt tcagcgatcg agacagagag aatatcgcgg
     2881 atagcaggcg tacgggttgc ccaggacgcc attaacaggc ctggcaaaaa gaagaacata
     2941 aacagcgccc aggttcggcg tttcaatgca ttacgtgaag aatttacggt catagatcac
     3001 gtcaaaataa gaagagggaa gacaacatta gcaaggttgt gtacatttgt acacaattgc
     3061 agacagagga aatgacatgc gtcgcgctaa cgatccgcaa cggcgagaaa aaattatcca
     3121 ggccacgctg gaggcggtga aactttacgg aatacatgct gttacgcacc gcaaaattgc
     3181 tacccttgcc ggggtaccgt tgggatcgat gacctactat ttttcaggaa ttgatgagtt
     3241 gttgctggag gcgttcagca gttttactga gattatgtcc cggcaatatc aggcattttt
     3301 tagcgatgtt agtgatgctc aaggcgcatg ccaggctatc accgatatga tctacagctc
     3361 acaggttgca acgccggata atatggagct gatgtaccag ctctacgcgc tggctagccg
     3421 aaaaccgcta ttaaaaacgg taatgcaaaa ctggatgcag cgcagtcagc aaacgctcga
     3481 acaatggttt gaacccggaa ccgcccgcgc gcttgatgcg tttattgagg ggatgacgct
     3541 gcattttgtc accgaccgta agccgctatc gcgcgaggag attttgagga tggttgagag
     3601 ggttgcaggg tagtagataa attttagata acaaaaaacc catcaacctt gaaccgaaat
     3661 ggcggggttg atgggctcca caaaatgggg acatcaaaga aaagcagtgg cactaattaa
     3721 gactgatgcc ctgcggaaaa gttctgcggt tgtgcaaaaa aatttcattt tcagggcaac
     3781 ttcagtttta tcctaatcct ggccatacca tgacgatgat tgtccctgcc agcgtcagca
     3841 ggacgttggc gattgcgtag gtgcccgcat agcccagcgc agggatgtta ctgcgagctg
     3901 tatcactgat gatctccatt gccggcgcgc aggtgcgtgc gcccatcatt gcgccgaaca
     3961 acagtgcgcg gttcattcgc aatacataag caccgaacaa gaaacagata accacgggca
     4021 caagactgac aattaatccg gcaatcaaca tctgaccgcc aatcgcgccc aggccgttat
     4081 taataccgct accggcgctc agaccaacgc ctgccataaa caccatcaag ccgaactctt
     4141 tcaccatgct taatgcacct tgcggaatgt aaccgaaggt cgggtggtta gcacgcataa
     4201 agcccagcat aattccggcg aataacaacc cggcagcgtt ccccatgccg aaactgaatg
     4261 tgctgaactg gaaggtgatc atcccgatca tcagcccaat aacaaagaag gcgcaaaatg
     4321 ccagcaggtc agtgacctgg ctgtgaatcg agataaagcc gatgcgatcg gcgatggttt
     4381 ttacgcgacg ggcatcaccg ctgacttgta aaacgtcacc tttgttaagt acgacgttgt
     4441 catctatcgg catctcaatc tggctacgaa tgacgcggtt aaggaagcaa ccgtgatcgg
     4501 tcaacttcag ttgtgcgaga cgtttaccta cagcgttatg gtttttaacg accacttctt
     4561 cagtgacgat acgcatgtcg agaaggtcac gatcgaaaac ttctttaccg ttacggaagc
     4621 tgggatcgag tcgggcatgg gcgtcgggat agcctaccaa cgctatttca tcgcccattt
     4681 gtagcacggc atcaccgtct ggatttgcca gaatcccgtt acgtcgaata cgttcaatgt
     4741 agcagccggt ttgtcgataa atacccagtt cacgcagatt tttgccgtcg gtccaggcca
     4801 ccagctccgg gccgacgcga taggcgcgga tcaccggtaa ataaacctta cggttggcat
     4861 cagtgtccag gccacgttcg cgggcgattt gctgggcgct ggtctgtaag tcctgatgct
     4921 gcaatttcgg caagtaacgc gcaccaacaa tcaaactcac cagaccgatt aaataggtta
     4981 aggcataccc gaggctcaga ttatccagtg ccagtgagag ctgcctgctt tccatgccgg
     5041 aatgacgcag tgtatcgcca gcaccgacca gaaccggtgt cgacgtcata gagcctgcta
     5101 acataccggc cgtcaggcca atatcccagc caaacagctt acctaaccct aaggcgatca
     5161 ccagcgcact gccaaccatc accagtgcta acattaggta atttttccca tcgcgaaaaa
     5221 aaatggaaaa aaagttcggt ccggcttcga ccccgacgca gaaaataaac agcataaagc
     5281 caagattaag cgcatcggtg ttaatgctga aatgttgttg gcctaataac agcgatacga
     5341 ctaaaacgcc aatggaatta cccagttgga tcgaaccaag tcgtaacttt ccgagacata
     5401 gcccaagcgc gaggaccaca aataataaca gaatgtaatt cccatttaac aattcggcga
     5461 cgtttatatt cacggaggct aacttcttgt ttactagtaa gctgttgaaa gaaatggtaa
     5521 tttacgataa tgttttttac cagaattcag ggcgcagatt cattcagcgc acctaaacga
     5581 tagtaaagta acaatatatt ttactagtgt aatcacatta ggtatcaacg gctatatgaa
     5641 ttgcgttggc ctatattagc atggaatgcg aagcggcttt atcttactga acgccacact
     5701 ggcgaaaaat gtgttcgata gacgcagtgt caggaggaac gagtgaaaca taaacaacgt
     5761 tgggcggggg caatctgctg ttttgtcctc ttcattgtgg tgtgcctttt tctggcgacg
     5821 cacatgaaag gcgcttttcg ggctgccggg catcctgaaa tcggcttgct gtttttcatt
     5881 cttcctggag cagttgccag tttcttttca cagcgtagag aagtcctgaa acctctattt
     5941 ggcgcaatgc tggcggcacc ctgttcaatg ctcattatgc ggctgttttt ttcaccgacg
     6001 cgctcattct ggcaagagct ggcatggtta ctaagcgcgg tgttctggtg tgcgctgggg
     6061 gcactgtgtt tcttatttat cagtagtttg tttaaaccac agcacagaaa aaatcagtaa
     6121 agccctcaac gcgagggctt gtcagacgat caggcgtcca gattttcttt cacccatgca
     6181 gcaaaatcgg tatagccgcc gatatgttgc tgatcgacaa aaatctgcgg cacggtttct
     6241 acgggtttac ctgccttttg ttgtagatct tctttagtga tcccttccgc acgaatatct
     6301 acatactgat actgaaaatc atcgcgttca ttgctcaatt tctcagccag atcttttgca
     6361 cgcacacagt aagggcaacc cgaacgacca aaaataacgg tttgcattat ttctctcctc
     6421 atagatttat gcctgtaatg atcacgctaa aatgtattcg ctgaaagtag gtttaacctg
     6481 ttgcattaat tgctaaaagc tataactgtt aaacacaata cagtgaaaag ttttagactg
     6541 aaggctcact ttgcagaggg aagcgtatgc gcgcgatcgg taaattgcct aaaggcgtgt
     6601 tgatactgga atttatcgga atgatgctac tggcggtggc gctgctgtcg gtaagcgact
     6661 ccctgtcgct gcctgagcca ttttctcggc cagaagtgca gattctgatg atttttctcg
     6721 gtgttttgct catgcttccc gctgcggtgg tggttattct tcaggtggca aaacgtcttg
     6781 ccccacagct gatgaaccgt ccaccgcaat attcacgttc agaaagagaa aaagataatg
     6841 acgccaacca ttgaacttat ttgtggccat cgctccattc gccatttcac tgatgaaccc
     6901 atttccgaag cgcagtgtga ggcgattatt aacagcgccc gtgcgacgtc cagttccagt
     6961 tttttgcagt gtagtagcat tattcgcatt accgacaaag cattacgtga agaactggtg
     7021 acgctgaccg gcgggcaaaa acacgtagcg caagcggcgg agttctgggt gttctgtgcc
     7081 gactttaacc gccatttaca gatctgtccg gatgctcagc tcggcctggc ggaacaactg
     7141 ttgctcggtg tcgttgatac ggcaatgatg gcgcagaatg cattaaccgc agcggaatcg
     7201 ctgggattgg gcggggtata tatcggcggc ctgcgcaata atattgaagc ggtgacgaaa
     7261 ctgctgaaat taccgcagca tgttctgccg ctgtttgggc tgtgccttgg ctggcctgcg
     7321 gataatccgg atctcaagcc gcgtttaccg gcctccattt tggtgcatga aaacagctat
     7381 caaccgctgg ataaagacgc actggcgcag tatgacgagc aactggcgga atattacctc
     7441 acccgtggca gcaataatcg ccgggatacc tggagcgatc atatccgccg aacaatcatt
     7501 aaagaaagcc gcccatttat tctggattat ttgcacaaac agggttgggc gacgcgctaa
     7561 aaccgccacg tcgatgtatg atacgcgggc ttttgaccag gtctgacaga gaggtgcagg
     7621 gtgaaaattg ccatattgtc ccgggatgga acgctctatt cgtgtaagcg gctgcgtgaa
     7681 gccgctatac agcgcggtca cctggttgaa attcttgatc cgctttcttg ctacatgaac
     7741 ataaatcctg cggcgtcttc tattcactac aaaggccgca agttacccca ttttgacgca
     7801 gtgatcccgc gtattggcac cgccattacc ttttatggga cggcggcact gcgccagttc
     7861 gagatgctgg ggagctatcc gctcaatgag tcggtcgcca ttgcccgggc gcgtgacaaa
     7921 ttgcgttcca tgcaactgct ggcgcgtcag ggcatcgacc tgcctgtcac gggcattgcg
     7981 cattcgccgg atgataccag cgatttaatc gacatggtcg gtggtgcgcc gctggtggtc
     8041 aagttggttg aaggcacgca gggaattggc gtcgtgctgg cggagactcg tcaggcggcg
     8101 gaaagcgtga ttgacgcttt ccgcggtctg aacgcgcata ttctggtgca ggaatatatc
     8161 aaagaggcgc aagggtgcga tatccgctgt ctggttgttg gtgatgaagt ggtcgctgcg
     8221 attgaacggt gggcgaaaga gggctatttt cgttccaatt tgcatcgtgg cggcgcggca
     8281 agcgtcgcca gtatcacacc acaggagcgt gaaatcgcga taaaagccgc gcgaacgatg
     8341 gcgctggacg ttgctggtgt ggatattctg cgtgctaatc gcgggccgtt ggtgatggaa
     8401 gtgaatgcgt cgccggggct ggaaggaata gaaaaaacca ccggtatcga tatcgcgggt
     8461 aaaatgatcc gctggatcga acgccacgct acgacagaat attgcctgaa aacgggtggt
     8521 tagtcgcaat cacattactg atcatggttt tgcctgcgct ttttgcgtaa gctgtgccgg
     8581 tctttttatc gaaagaggtt gtacaaaatt atgacatcgc tggtcgttcc tggtctggat
     8641 acgctgcgtc aatggctcga tgacctgggg atgagttttt ttgaatgtga taactgtcag
     8701 gctctgcatc tgccccatat gcagaatttc gacggtgtct ttgatgccaa aatcgatctg
     8761 atcgataaca cgatcctgtt ttctgccatg gcggaagtcc gaccttcagc cgtattgccg
     8821 ctggcggcgg atttatctgc catcaatgcc agttcgctga ccgtgaaagc atttcttgat
     8881 atgcaggatg ataatctgcc aaagctggtg gtttgccagt ctttatccgt tatgcagggc
     8941 gtaacctatg agcagtttgc atggttcgtg cgtcagagcg aagagcagat ttcgatggtc
     9001 attcttgaag ctaatgccca tcaactgctg ttaccgactg acgatgaagg gcaaaacaac
     9061 gttaccgaaa actattttct ccactgataa ttcctttcga gcacgcagtc gctgccgcag
     9121 tgactgcgtg ctgcaaaatt atctgctgtt tttaaccttt tcttaaagat tatttcactt
     9181 ctcttgtgtc gatttggctt tatcacatag agcaaatacg cataaaaatt tgttaaatac
     9241 cgttttttaa tccgagctat agtctcaaac cctggctaaa gttattcttg cgatactttt
     9301 atatagcgag cagtgctggc cgggagagag ttctcttttc ttacaccgcg ccgataaaaa
     9361 aatatgcacg tttattgcat atctttcagt gtgacaactt ttgttcgttt gttaacgaac
     9421 tttcagaagg aaagagatgt gaccgcctta aataaaaaat ggctatcggg tctggttgcg
     9481 ggtgctctga tggccgtctc tgtcggcacg ctcgcggctg aacaaaaaac actccacatt
     9541 tataactggt ctgattatat cgcccctgac acggtggcca attttgaaaa agaaaccggt
     9601 attaaagtcg tctacgatgt tttcgactct aacgaagtac tggaaggcaa attaatggcg
     9661 ggtagcaccg gctttgactt ggtggttcca tctgccagct ttcttgaacg ccagttgact
     9721 gcgggagttt tccagccgct ggacaaaagc aaattgccgg agtggaagaa tctcgacccg
     9781 gagctgctga agctggtcgc caaacacgat cccgacaata aatttgctat gccctatatg
     9841 tgggcgacga ccgggattgg ctataacgtt gataaagtta aagctgtgct gggcgaaaac
     9901 gcgcccgtcg atagctggga cttgatcctc aaacctgaaa atctggaaaa actgaaaagc
     9961 tgcggtgtct ctttcctgga tgcgccagaa gaagtttttg ctaccgtgtt gaattatctc
    10021 ggcaaagatc ccaacagcac taaagcggat gattacaccg gaccggcaac cgatctgctg
    10081 ttaaagctgc gcccgaacat tcgttatttc cattcatctc aatacattaa cgacctggca
    10141 aacggcgata tttgcgtcgc tatcggctgg gcaggtgatg tctggcaggc gtcaaaccgc
    10201 gcgaaggaag cgaagaatgg agtgaatgtc tcgttctcga ttccaaaaga aggggcgatg
    10261 gcgttctttg atgtattcgc catgcctgcg gatgccaaaa acaaagacga agcctatcag
    10321 ttcctgaatt acctgctgcg cccggatgta gtagcgcata tttccgacca tgtgttctat
    10381 gccaacgcca ataaagcagc cacgccgctg gtgagtgcgg aagtccgtga gaacccgggt
    10441 atttatccgc ctgcggatgt tcgtgcgaag ctgttcactc tgaaagtgca ggacccgaaa
    10501 atcgaccgtg tgcgcacccg cgcgtggacc aaagtgaaga gcggaaaata atccgcagcc
    10561 ttagatgccg ggcgggcgca ccacacccgc cggcaattcg caccatcatg gtgcgcttgc
    10621 acacattcaa tgccggagag cagccgtgaa tgacgctatc cctcgcccgc aggcgaaaac
    10681 ccgtaaggcg ctgacgccgc tattagaaat ccgcaacctc accaaatcct acgatggtca
    10741 acatgcggtg gatgatgtca gcctgaccat ctacaaaggt gaaatcttcg cgctgctggg
    10801 cgcatccggc tgtggcaagt ccacgctgct gcgtatgctg gcaggtttcg aacaaccttc
    10861 tgccggacag ataatgcttg atggcgtcga tttgtcacag gttccgcctt acctgcgccc
    10921 catcaatatg atgtttcagt cttacgcgct gtttccgcat atgaccgtgg aacagaacat
    10981 cgcttttggc ctgaaacagg acaaactacc gaaagcggaa atttccagtc gggtcaatga
    11041 gatgctcggc ctggtgcaca tgcaggagtt cgccaaacgt aaaccgcatc agctttctgg
    11101 tggtcagcga cagcgtgtgg ccctggctcg aagccttgcc aagcgcccga aactattact
    11161 gctcgacgag ccgatgggcg cgctggataa aaagctgcgt gaccggatgc agcttgaagt
    11221 ggtggatatt ctggagcgcg tcggtgtgac ttgtgtgatg gtcacccacg atcaggaaga
    11281 ggcgatgacc atggcggggc gcatcgccat tatgaatcgt gggaaatttg tccagattgg
    11341 cgaaccggaa gagatctacg agcatccgac tacccgctat agcgccgaat ttattggctc
    11401 ggtaaacgtc tttgaaggcg tactcaaaga gcgtcaggaa gatggcctgg tgcttgattc
    11461 gccggggctg gtgcatccac tgaaagtcga cgcggatgcc tcggtggtcg ataacgtgcc
    11521 ggtacatgtg gcgctgcgcc cggaaaaaat catgctttgc gaagagccgc ccgccaatgg
    11581 ttgtaacttc gcggtggggg aggtgataca cattgcctat ctcggcgatc tttcggtgta
    11641 tcacgttcgt ctgaaaagtg ggcagatgat tagcgcccag ctacaaaacg cccatcgtca
    11701 tcgtaaaggg ttaccgacct ggggcgacga agtgcgtttg tgctgggaag tggacagctg
    11761 tgtggtgctg acggtttaag gagcaaagat gaatacactt gaacctgctg cccagtcgaa
    11821 accgccgggc ggatttaagc tgtggttgtc gcagctgcaa atgaagcatg ggcgcaaact
    11881 ggtcattgcg ttgccatata tctggttgat cttgctgttt ctgctgccat ttctgattgt
    11941 ctttaaaata agcctggcgg agatggcgcg cgctattcca ccttataccg aactgatgga
    12001 gtgggctgac gggcaactgt ccatcactct taatctcggt aattttctgc aactgaccga
    12061 cgatccgctc tatttcgatg cttatctcca gtcgctacag gtggctgcga tttcgacatt
    12121 ttgctgttta ctgatcggct atccgctggc gtgggcggtg gcgcacagta agccttcgac
    12181 ccgtaatatt ttattactac tggtgatcct gccgtcgtgg acctcgtttc tgatccgcgt
    12241 ttatgcctgg atgggaatat taaaaaacaa cggtgtgctg aataattttc tgctgtggct
    12301 gggggttatc gatcaaccgc tgaccattct tcataccaat ctggcagttt atattggcat
    12361 tgtttacgcc tacgtgccgt ttatggtgct gccgatttat accgcgttga ttcgtattga
    12421 ttattcgctg gtggaagcag cgctggatct cggtgcacga ccgctgaaaa cgttctttac
    12481 tgtgatcgtg ccgctgacta aaggtgggat tattgccgga tcgatgctgg tgtttatccc
    12541 ggctgtgggc gagtttgtga tcccggaact gctcggtggc ccggacagca tcatgatcgg
    12601 gcgcgtgcta tggcaagagt ttttcaataa ccgcgactgg ccggtggcct cggcggtagc
    12661 gatcatcatg ttgctgctgc taattgtgcc gataatgtgg ttccacaaac accagcaaaa
    12721 aagcgtggga gaacacggat gaataattta ccggtagttc gttcgccctg gcggattgtg
    12781 attttgctgc tgggctttac ctttctctac gcgccaatgc tgatgctggt tatctattcg
    12841 tttaacagct cgaagctggt gacggtgtgg gccggctggt caacgcgctg gtatggtgag
    12901 ttattgcgcg atgacgcgat gatgagtgcg gttggtttaa gcctgacaat tgctgcctgt
    12961 gcggcaacgg cggcggcgat cctcgggact attgcggcgg tggtgctggt gcgctttggc
    13021 aggtttcgcg gatcaaatgg ctttgccttt atgatcaccg cgccgctggt aatgccagat
    13081 gtcatcacgg gcttgtcgct gttgttatta ttcgtcgcgc ttgctcatgc cattggctgg
    13141 cctgcggacc gcggtatgct taccatctgg ctggcgcatg tcactttctg tacggcttat
    13201 gtggcggtcg ttatttcgtc gcgtctgcgg gaactggatc gctcgataga agaagccgcg
    13261 atggatctcg gtgcgacgcc gctgaaagtg tttttcgtca ttacgctacc gatgatcatg
    13321 cccgcgatca tttctggctg gttactggct tttactttgt cgcttgatga tctggtgatc
    13381 gccagctttg tttccgggcc gggagccacc acgttaccga tgctggtctt ttccagcgtg
    13441 cggatggggg tgaatccgga aatcaacgcc ttggcaacat tgatcctcgg cgcggtcgga
    13501 attgtcggat ttatcgcctg gtatctgatg gcgcgcgcag aaaaacagcg gatacgcgac
    13561 atccaacgtg caagacgtgg ctgaagacac taaaatttgc caacctggct acataatgcc
    13621 gcgcatgtcg cggcattgtt ttcatggaag acgaaacgtt gggatttttt aagaaaacat
    13681 cttcatctca tgctcgcctg aatgtgcctg cgctggtgca ggtggcggcg ctcgccatta
    13741 ttatgatccg tggcctcgac gtgctgatga ttttcaatac gctgggcgtg cgcggtattg
    13801 gcgagttcat tcatcgcagc gtacaaacct ggagtttaac gctggtcttt ttaagcagtc
    13861 tggtgctggt tttcattgag atctggtgtg cgttttcact ggtgaaaggg cgtcgctggg
    13921 cgcgctggct atatctgctg acacaaatca ccgccgcaag ttacttgtgg gcggcttcgc
    13981 tggggtacgg ttatccggag ctgttcagca ttcccggtga atcaaaacgt gaaatcttcc
    14041 atagcctgat gctgcagaag ctgccggata tgctcatcct gatgctgctg ttcgttccct
    14101 cgaccagtcg gcggttcttc cagttgcaat aatgtgtata atcgtcgccc ctgatgatgt
    14161 gaaggtcaat gtatgcagtg cgcactttac gacgcgggtc gctgtcgttc ctgtcagtgg
    14221 ataacgcagc cgattccaga gcaactctcc gctaaaaccg ccgatcttaa aaatctgctc
    14281 gccgattttc cggttgagga atggtgcgcg ccggtgtcag gcccggaaca agggtttcgt
    14341 aataaagcca aaatggtggt gagtggtagc gttgaaaaac cactgctcgg tatgctgcat
    14401 cgcgatggca cgccggaaga cctttgtgac tgcccgcttt atccagcctc atttgcgccc
    14461 gtttttgcgg cgctaaaacc gtttatcgcc cgagcggggt taacgcccta caacgtggcg
    14521 cgtaagcgtg gcgaactgaa atacattctg ctgactgaaa gccagagtga tggcggcatg
    14581 atgctgcgct ttgtactgcg ttctgatacc aagctggcgc aactgcgtaa ggcgctgccg
    14641 tggttacacg aacaactacc gcagctgaaa gttattaccg tcaatattca gccggtacat
    14701 atggcgatta tggaagggga gacggagatc tacctgaccg aacaacaggc actggcggag
    14761 cgttttaatg acgtaccgct gtggatccgt ccgcaaagtt tcttccagac taatccggcg
    14821 gtcgccagcc agttgtacgc caccgcgcgc gactgggtac gacagctgcc ggttaaacat
    14881 atgtgggatc tgttctgcgg tgtggggggc tttggtttac actgcgcgac gcctgacatg
    14941 cagttaaccg ggatcgaaat tgcgccagag gccattgcct gtgcaaagca gtcagccgct
    15001 gaactgggct taactcgttt acaatttcag gcgctggact ccactcagtt tgccactgct
    15061 cagggggagg tgccggagct ggtgctggtt aacccgccgc gccgcggcat tggtaaaccg
    15121 ctgtgtgatt atctttcaac gatggcaccg cgttttatta tctactccag ctgtaacgcc
    15181 caaaccatgg cgaaagatat ccgcgaactg ccaggttacc gcatcgaacg ggtacagctt
    15241 tttgatatgt tcccgcacac cgcgcactat gaagtgctga cgctgctggt gaagcaataa
    15301 aaaagccaca ggtgcggctt cagattgctg atcaggtgcg ctttgttcat gccggatgcg
    15361 gcgtgaacgc cttatccggc ctacaaaatc atgctaaatc aatacattgc agagttgatg
    15421 tgggccggat aagcgcagcg catcaggcaa tcttactttt gtcatcagtc tcaattactg
    15481 cggaaaccac tggtcattga ttttctgata tgtaccgtca gctttaattg ctgccagcgc
    15541 gttattcagt ttttccagca gagctttgtt atccggacgt acagcgatac ccaggccggt
    15601 gccgaagtat tgcggatcgg tcactttttc agtcgcaacg cccagttgcg gattggtttt
    15661 cagccattcg tttaccaccg cggtgtcacc aaatacccca tcaatacgac catttttcag
    15721 atcgataaag gcattctgat aactgtcata ggagacagtt ttcacttccg ggtgctgatc
    15781 ctgaatatat ttctggtgcg tcgtaccgtt ttccatccca atgcgtttgc ctttcaggtc
    15841 ggcaaacgtt ttgtaggtat cttttttggc aatcacgacg gctgagtttt catagtaggg
    15901 tgtggtaaac gaaacctgct tgctacgctc cggggtgata tccataccgg agattacggc
    15961 gtcatatttt ctgaatttca gtgacgggat caggctgtcg aacgcgtgat tagtaaaagt
    16021 acattctgcc tgcatttgtt tgcacaaggc ttttgccaga tcgatatcaa agccgacaat
    16081 ctcattatta gcacctatag attcaaaggg tggataggtg gctgaaacgc caaaattgat
    16141 tttctctgcg gcagaagcac cgaaagtaaa ggaagcaagt aaagcggcaa gaactaactt
    16201 tttcatgatg gaactcccgt ctgtcaatct tatgattttt ggccgtgtct gcggcatggg
    16261 ataacaatgc caccaagtga atttatatgc aataaacatg attaaataat ttaaatgaaa
    16321 taaaaaaaga cggataactt agtgagttgt ccgtcttcat tataagaatt tatgcactat
    16381 gtaggccgga taaggcgtcc acgccgcatc cggcacaggc accgtgctga tgtctgatgc
    16441 gacgctggcg cgtcttatca gacctacaaa cccccccggc gaatgtacgc agctacatta
    16501 attccgccgt tcgaatgcca gtgctttgcg ctcgatcaga cgcatcatca gcgtcagcag
    16561 gccgttgacg accaggtaaa taatccctgc cgcaccgaac accattacat cgtaggtgcg
    16621 tccgtacaac aactggctgt atcccatcac ttccatcagc gtaatggtgt aagccagaga
    16681 ggtacttttg aacaccagca ccacttcgtt ggaataagaa gagagcgagc gtttaaaggc
    16741 atacggcagc aggatcgcct gcgtatcttt tttgctcatt cccagggcgc tacaggactg
    16801 ccactgacct tctgggatcg cacgaattgc accgtaaaac agctgcgtgg tatacgccgc
    16861 actgttcagc gataacgcaa tcagcgcaca taaccacggt tctgacaaca aatgccacag
    16921 tgccggatac tcctgcaacg tcggaaactg gcccggcccg taataaatca ggaagatctg
    16981 caccagcagc ggcgtaccgg taaacagcgt gatataaccc cgcaccagcc acaccagcac
    17041 cggcgttttc agcgtcagga tgatggtaaa aatcaatgcc agaatcagtg ccacaatcag
    17101 cgaggcaaag gttagcgtca ggctggtgtg cagccctttc atcagctcgg gtaaatactc
    17161 aaacattagc tgggcctccg ctcaaaacgt gtcgcgcgca ggtcaatgcg tttgagaatg
    17221 tactgactga gcagggtgat caccaggtaa atcgccgccg ccacaatgta ccaggtaaat
    17281 ggttcctggg tacgagtagc aatgcttttg gtttgtaaca ttaaatcatt cacactaatc
    17341 aaactgacca gcgcggtatc tttcagcagc accagccact ggttaccgag gccaggcagc
    17401 gcatgacgcc acatctgcgg catcaccaga cggaaaaaga tagccgattt cgacagcccc
    17461 agcgcctgac cagattccca ctggcccacc ggtacagctt tcaacgcgcc gcgcagcgtt
    17521 tgcgaggcat aggcggcata cagcagtgac agagcgatga caccacaaag gaacggactc
    17581 acgtcgaagt tctcaatgtc catctgcacc gggatctgca cgaacccaag attgatagtg
    17641 aagccatacg aaagcgtcag cagtaactgc gaggagccaa aatagataaa cagcactacc
    17701 agaatttctg gcaggccacg cagaatggtt accagcgctg aacctgccca cgcgacagga
    17761 cgccatttcg ctgactccca taccgcaaag aacatcgcca gcgccagccc gacaatcaat
    17821 gcacaaacgg caaggccgac ggtcatcccg gcggcgcttg ctaaaggaaa aaattcattc
    17881 atcaggaatt acttctggaa ccatttgttg tagatggttt cgtaagtgcc atctttcttc
    17941 actttttcca gcgcagtgtt gagtttctgc tgcagttcag tgttgccctg acgtaccgcg
    18001 atgccgaggc cagtaccgaa ataatcttta tcggtcactt tatcgcccac tgccgccagt
    18061 tttgggttat ctttcagcca ctcagtgacc actgcggtgt caccaaatac gctgtcgata
    18121 cgcccgtttt gcagatccag ttttgcgttc tggtagctgt catacggaac ggtggtgatt
    18181 tccgggtgct tatccataat gaacttctgg tgtgtcgtcc cgttctgaac gccgactttt
    18241 ttgcctttca gctgatcaac actggtgtat ttgccttgct gacccacaaa cagggcagag
    18301 ttgtcatagt acggggtggt aaacagtacc tgcttttcac gctccggagt gatatccatg
    18361 cccgccatca ctgcttctac gcgacggaat ttcaggcttg ggatcaggct gtcaaacgcc
    18421 tggttagaga aagtacaggt tgcatcaatc tctttacaca gtgcctgtgc caggtcgacg
    18481 tcaaaaccaa cgatctggtt gtttgcatca atcgattcaa acggaggata ggaggcttcg
    18541 gtagcaaaac gaatggtttc ggcagctgtg gcggaaagac taaaacctgc aattaacgcg
    18601 gcaatcagaa cttttttcat tgttgttatc ccgaatctta gtgagagaga tagtttttaa
    18661 atgcttcggt ttgcggctcg gtaaagcagc tcgcgtcgcc ttgttctacg atatgaccat
    18721 tttccatata caccactcgg ctggcggttt tacgcgccac ttcaacttcg tgggtgacga
    18781 tcacctgggt aatattcgtt tctgccagct cacgaatgat gctgacgatt tgtgccgtaa
    18841 tttccgggtc cagtgcggcg gtcggttcat cgaacagcag tacctgcggt tccatcatca
    18901 acgcacgggc aatagcaaca cgctgctgct gaccaccaga aagatgcagc gggtaacgat
    18961 cgctataagg tttgagacgc agacgttcca gcagtttttc tgcacgggcc agcgcctgat
    19021 ctttactcaa ccccagtaca ctgcagggcg cttcaatcag gttttgctgc acggtcagat
    19081 gcggccacag gttgtattgc tgaaacacca tgccaacgtt acgacgcaaa tcgcgaatcg
    19141 ctttgtcaga gggtgttttg gtgaaatcga aatggttgcc tgcaatgttg agcgtaccgg
    19201 agcgcggcat ctcaagcaga ttgagtacac gcagcagcga gcttttaccc gcgccgctgg
    19261 ggccaagtaa caccagcgtt tcgccctgtg ggcaatccag cgtgatatcg aacagcgcct
    19321 gatgcgcgcc gtagaagcaa ttaatgccgt ttaattgaat actcattgac actcgtatac
    19381 tggcagtctg atagctattg aggtcgaaga tagtaccttt gacagaataa ttatgcaata
    19441 tttatgcttt aaaagttaaa agcaaagcgc attattcaat aaacatagca caaaataacg
    19501 ggggcggtgg tcggtgagca taaatgtcgg cattcctcac gaaatgccgg ataatttacg
    19561 gggtttattg gttgatcaag gcgttagcga ttctcgatgg actgacggag cgtacccgcc
    19621 gtggcatgaa cgctaccgcc taagtagcgc acatcgtcga tgacccaaca ctggccttcc
    19681 tgaatcatta acacttcatc ctgccagccc tggtcaccct gtttgagatc cacccgcaat
    19741 ggaatgttac gggcatcacg attagggata gtcgatgcac tggcaacgtg ggcgctgtct
    19801 ggcaaggtgg ttcgactgga gaacggatcg ctgcttaaca attcacgatg actattatcg
    19861 cggctggcat cgctaagttg ggttgccagt ttgtcactca aataggggcg cagggcggta
    19921 atgtcattgc tgcgatgttg aatgcggtag tcgtagaatt gttgagccac actgtcaggc
    19981 cctccctgga cacaggaacc ggtgcggggg ccgttatctt tataagctgg cgtgacggtg
    20041 gtacaggcac tcagcagcag tgcacatgga ataagtagag atagtgaacg gtagcgcata
    20101 atcatttcct tattagcttg ctgtctgcgt cacactacga ccaaacgcta tcagattatt
    20161 taatacattg tttttaatac aattctgaaa caatctgaac acctgattgg gtttgagtcc
    20221 attgctggct acccactcaa ggttgctcgt agctgactta cttaaattac ccaaacaata
    20281 gacagtgtaa accatttgtg aggttgagcg tgttatatgg ctgaaatgat catttaaggt
    20341 aaatatgata gttctgaaat ggtggtataa atcctcctgt agaggttaag agcatctgca
    20401 agaagccagt ttcacattta taacgcgtga taaatgaatg cggttattgt ctaatcatta
    20461 caaaattttt cacatgcgat aagagatcct gcgattaaga tgattgccaa aattacgttg
    20521 tatttttgaa aatgagcgaa atttgatagt cccagctttg actctcgata atattattta
    20581 tgctacaatt gtgggtaaag aatttaagct aatgaatttg agtgaagaat aaattaaatt
    20641 gcttcagtat tttgttaatt aatatgaata caaaaggcgt tgtgctttta taattataaa
    20701 gcgcgaggag ttcggtggat tatcgttttt cttgggtgat ttttaatatg ataaacaata
    20761 tatctgatca agcatcatca tttccaggca cacaactaaa tcaaagtgat aatttcctgg
    20821 attcactgag ggaatttttc gcgatattaa attccagtcg taagggtgag ttgtctacat
    20881 gggataccat ctacttgcat ctgatattag caataaatgc tgacagtgat ctcataaaaa
    20941 atgatgtgtt gctcgcggaa aatattccat cagcgaatta ccaatttaat acatttttta
    21001 gcaatgcttt cgagattgat gttaaaaaat atctgggtaa atcagaagat aacgaagttg
    21061 aaataaaagc gggtaatgaa agaatctcta ttggaatacg taatgtatcg aatggaaaac
    21121 ttgaaagaca acagtttctt ttcccactgg actatgagaa taaattacaa gaacaattag
    21181 ataagtattt tacgattgaa tcgcacccat tattatacag gtataccatt ggtagtaaga
    21241 ttgcgaatgt gatttttgaa aagttataca gtcgtattga ctttaacaaa gaacaatata
    21301 tatcctttat aaaggacgca tttattcatt tttatgatta tagcaggcga tatgctatta
    21361 gtgagaatat cgataaagac gctgtaacaa acaatatcgc attgatgagc actttttatg
    21421 acagtgataa tacgtcaggt gaggttttaa ataatgattt tacagaagaa gagtcatttg
    21481 aaactgcgtt agatgtagag catgcaatag tgcttggatt tgctgatgat aattttgaaa
    21541 caaaacctgt tcattatcag gatttattaa cacgatttag tgcctttcaa gataccgtgt
    21601 ttaatctgtt tcctgaaatg cactcttcgc attaccacga tatttgtagc gtaagtgtgg
    21661 atatgacaaa gggaacgcaa tgcatgattc acttgatggt gaatgaagaa gttttcatgt
    21721 ctctgcctgt tccagttgcc acgatggtac gtgaagatgc aagtaattta gtcaatctta
    21781 aaacactact caatgatggt tgtttcatta aatatagtca ttttaatgac gtggcattaa
    21841 taaagcaaaa cataagtaat ctctatttat cacacactgt tgtgaatgaa agcatattga
    21901 aaaaatgttg ttttgaaaac ggcagtttag gtgatgttaa aataacaaat agcaatgtaa
    21961 taaacagtgc attcaaaaat atttcgttta gatctgttaa aattaataac gtaaatactc
    22021 attctttaaa atttattaac tgcaattttt tcaatgttga tatgataaga gttaatctca
    22081 gcaaatgtct ttttcatgaa tgtagtatgc atggtgtcaa gataaaaccc tggttgcctg
    22141 taaaatggac aaaagaatta ataagtgatt atttgtatgg ctgtttacta tctctgtact
    22201 ctatctgtgc aagggatata tataatatga acgccgggaa taacgtgaag gttgctgcgg
    22261 atgctttcct tgaaattatt ttctctctta aaaataaata ttgtattaaa ctattaagcg
    22321 ctcaggatcg ggcgtttata tacgagtttg caagaatgat cttcgcttat attaatgaca
    22381 aaagcattga gatcttatta ctttcttgtt ttgcggcggc tgaccaaaaa gcaatccagc
    22441 ggtatagacc tcagtcacaa gatggggaag actttaggag tcatttacaa tataagttgc
    22501 ctctttccgc tcattaatgt tgttggtgca acataataag acaggtaagc ggtaccctat
    22561 aatgaaacca atatcgggtt atgcaacgga taatcgtaaa gacttaagtg ggtgtttttg
    22621 acacaatgag atgatttcct aaactgatca aaaccttctt cccaacatgg catatcagtc
    22681 cgataatgct ttagagatcg aaggcttaac aaacaaggag atcgatcatg caattttcta
    22741 caaccccaac tctggaaggc cagaccatcg ttgaatattg cggtgtggtg accggcgaag
    22801 cgattttagg tgccaatatt ttccgtgatt tctttgccgg tatccgcgat atcgttggcg
    22861 gacgttccgg tgcgtatgaa aaagaactgc gtaaagcacg ggagatcgcc tttgaggaat
    22921 taggctccca ggcgcgggcg ctgggggccg atgccgtagt tggtattgat atcgactacg
    22981 aaacggtcgg gcaaaacggc agtatgctga tggttagcgt cagcggtacg gcggtgaaaa
    23041 cgcgtcgatg agaagagtct tctggctggt cgctgccgct ctgttactgg cagggggtgc
    23101 aggcgaaaaa ggcattgtcg agaaagaggg atatcagctt gatacccgac accaggcgca
    23161 ggcggcgtat ccgcgcatta aagtgctggt gatccactac accgcagatg attttgatag
    23221 ctcgctggcg acactgactg ataagcaggt cagctcgcat tatctgatcc ctgcggtgcc
    23281 accgcgatac aacggtaaac cgcgcatctg gcaactggtg ccggaacaag aactggcctg
    23341 gcatgcgggg attagcgcct ggcgcggggc aacgcgcctt aacgacacct ctattggcat
    23401 tgagctggaa aaccgtggct ggcaaaaatc atccggagtg aaatattttg ccccgtttga
    23461 acaggcacag attcaggcgc ttattccgct ggcgaaagat attattgccc gttatcacat
    23521 caagccggaa aacgtagtgg cacatgcgga tatcgcaccg cagcgcaaag acgatccggg
    23581 gccattattt ccctagcagc aactggcgca gcaggggatt ggtgcctggc cggatgcgca
    23641 gcgggtgaac ttttaccttg ccgggcgcgc gccgcacaca tcggtagata ccgcgtcatt
    23701 gctggagctt ttggcgcgct acggttatga cgtaaaacct gatatgacac cgcgcgagca
    23761 gcggcgcgtg attatggcat tccagatgca tttccgcccg acgttatata acggcgaagc
    23821 ggatgcagaa actcaggcga ttgccgaagc attgctggag aaatacgggc aggattagcg
    23881 cggcagtttt ccgtggtcgc gtagccaggc ggcagttttc tcgatgcctt catccagggt
    23941 gatgaccggc tgataaccta actcttcctg cgcacgcgta atatccagcg taaagtcaaa
    24001 attcaacttg gagacgccgt agtgggtcag cggcggctct tttgctgact tgcggcctaa
    24061 acgctccatg ctgcgggcga tcatatccag catcgggtaa gggacggaac gaatacgaca
    24121 gtcaatattc aactcgtcga tcagcttctg cacgatgctg cgcagtgtgc gatgctcgcc
    24181 gttggtgatg ttgtacacac gcccggaagg tagcttatcg caggcttcct ggcttgccag
    24241 ccacattgcg tgcacggcat tttcatagta ggtcatatcg accagcgcac tgccgccatg
    24301 cggtaacaga atactgccgt agtggtgcat catatgcgcc agacggggaa taaagacttt
    24361 atcgtgcggt ccgaacagac tttgtgggcg cagaatagta aagcgcgttt gtggattcgc
    24421 ctgcgaaagc atattgatca cttcttcgct ggctgctttg ctgcgggcaa actcgttggc
    24481 gaagcggtga gggcgaaaat cttctttaat atcgcgatgg tggtgataat cgaagtacag
    24541 ggagggggaa gagatatgaa taaagttacg cacaccccag gcgacagccc attcacccag
    24601 gcggcgagtg gcgcgaacgt tagccagatc gaaagcctgt tgtgtccccc agggtgaggt
    24661 aaagctggag cagtgccaca gcgtatcaat gcccgcgagc atcactttag cttgtgatga
    24721 aaccagctcg gtcagatccg tcggaacaaa ctctgcgccc attttttcca gcaatttacc
    24781 cattgcttcg ttgcgaccgc tcgcacgcac gctgatgcct ttctggcata aaaactctac
    24841 cgcgtttcga cctaagccgc tggtggcgcc ggtaaccagt accttcatat caatccactg
    24901 ttgttgagaa aatgacgtgc gcattcttcc gtgatttccc ccatgatgca atgggaaaca
    24961 tgaaagaata acgcaggttt tgtcgattaa tctgtgcttt gttctgccag tctggcgatt
    25021 tgttttgcca ttccgcgaaa aataaacaga tgcgcgggga tcatcaataa ccagtaaaac
    25081 agccccggca taccgtgcgg atgccagaaa gcgcggacat cgatagtacg atagtcacct
    25141 ttatcttcca gggtaaaaca cagtcgtccc agccccggcg ctttcatgcc aaataacaac
    25201 gtaagttgct ttttcggttc aacgacaatc actttccagc tatccaccgc atcgccggtc
    25261 tgtaaatatt cgcgctccgg gcggcctttc gccagattat gaccgatcgc gcggtccatc
    25321 aacgcccgtg tctgccacaa aatattgcca aagaaataac gctctttacc gccgatttgg
    25381 ttcaccacca gccataaagc cgcaaggctg gcggacgttt taacggtaaa ccccgcctgt
    25441 ttggcgaaat aaccgtactc cggtcgccag cgggcaaagg cctgagcgtc gtagccccag
    25501 tcgctggagt tgaccagttt ttcttcctct ttcaacgtgc tacgtaccgc gtcatcgaaa
    25561 gcgatcagcc gttgtgggat gagtgcacgt agcgcggtat catctgccag cagatcgtgt
    25621 ttcagcccct gaatcaacgc cctggcggtg gtgggcggta cggaagtaat cacattgaga
    25681 aaccacaccg aaatccagcg ggtggggagg gggatgggga tcaaccagcg gcgcttaccg
    25741 ctcaccgcca taaaatgttc aaactgttgc tgataactga gcacctctgg tccggcggct
    25801 tcgaagatgc ggtgttcgtt ggctggatgg tctaacagcg ccaccagata gtgcagcaag
    25861 ttttccagcg cgatgggcgt ggtgcgtgaa cgtacccagc gtggcggcgt taacactggc
    25921 aggttgtaga ccatatcgcg catgacttcg aacgccgctg aacctgcgcc aacgataatt
    25981 ccggcccgaa gttcggtcac tggtacattc gcttcacgaa gaatgtccgc cgtagcctga
    26041 cgagcacgca gatgatccga ctgctcatgt ggcggggcct gcaacgaact gagaaagatt
    26101 aattgcttaa ctggtacttc acgtagcgca tcgcggacgt tgagagccac ttggcgctcc
    26161 tgagcgataa aatcgccgcc ttcgcccatg ctgtgcacca gaaaatagac ggtatcgata
    26221 tcctgcaaca gggccggaag gttatccggc cagctgagat cgactttatg gcaactgacg
    26281 tttgccagtt gcagctttgc aagcctgtcg acatgacgtg ccgccgccag gatctgatgc
    26341 ccttgctggc tgagtgtgcg caccagatgc tgaccaatgt agccactggc accgagaact
    26401 aaaatgcgtt gcggcacgtc tctctcctta acgcgccagg aatgcgcgcc agtgggcggc
    26461 gacttccgcc agttgttcgc gcgagacgtc aagatgcgtc accaggcgga caatcggcga
    26521 ggcgttaatc agcacgtttc tcgctttcat gtattcgcct aacgcggcag cattttcttc
    26581 cccgacgcga acaaacagca tattagtgtc ctgacgcatc acatccgcgc ctgcttcacg
    26641 cagttgctcc gccatccagg cggcgttgtc gtgatcttcc tgcaaccgtg cgacgttatt
    26701 tttcagggca tacatcccgg cggcagccag aatcctggac tgacgcatcc cgccaccggc
    26761 cattttccgc cagcggatgg cacgtttaat gtaatcacga ttaccgacga gtaatgaacc
    26821 gactggcgtc ccaagacctt tcgacaggca aatggtgaac gaatcacaat attgcgtgat
    26881 ctctttcagt tcgcagccgt aagccaccac ggcattaaag atgcgcgcac cgtcaacatg
    26941 cagcgccaga ttgcgctcgc gggtaaattc ccatgcttct ttcaggtatt cacgcggcag
    27001 cactttgccg ttgtgggtgt tttccagact gagtaatttg gtgcgggcga aatggatatc
    27061 gtcgggtttg attttcatcg ccactttatc cagcggtagc gtgccgtcgg cagccgcgtc
    27121 gatgggttgc ggctgaatac tgcccagcac cgctgcgcca ccggcttcaa acagatagtt
    27181 atgcgcggcc tgaccgacaa tatactcttc gccacgttcg cagtgactga gcagagcgac
    27241 caagttggcc tgagtgccgg taggcagaaa aatggcggct tctttaccgg aaagctctgc
    27301 tgcatagtcc tgcagagcat taacggtagg gtcgtctccg taaacgtcgt ccccaaccgg
    27361 ggcggccatc atcgcttcga gcatggcgcg gctcggtcgg gtaacggtat cactgcgtaa
    27421 atcaatcatg gcatgtcctt attatgacgg gaaatgccac cctttttacc ttagccagtt
    27481 cgttttcgcc agttcgatca cttcatcgcc gcgtccgctg ataattgcgc gcaacatata
    27541 caggctaaaa cctttggcct gttcgagttt gatctgcggt ggaatggcta attcttcttt
    27601 ggcgaccacc acatccacca acaccggacc gtcgatagag aaggcgcgtt gcagggcttc
    27661 atcaacttca gaggcttttt ctacacggat acccgtaatg ccgcacgctt cggcaatgcg
    27721 ggcaaatttg gtgtcgtgca gctcagtacc gtctgtcagg taacctccgg ccttcatctc
    27781 catcgccaca aagcccagca cgctgttatt aaagacgata attttcaccg gcagtttcat
    27841 ctgcactact gagaggaaat cgcccatcaa catgctaaaa ccgccatcgc cgcacatggc
    27901 gaccacctga cgttccggct cggttgcctg cgcacccaac gcctgcggca tggcgttagc
    27961 catcgaaccg tggttaaacg aacctaacag gcgacgcttg ccgttcattt tcagataacg
    28021 tgccgcccat accgttggcg tgccgacgtc acaggtaaag atggcgtcat cggcggcaaa
    28081 atgactaatt tgctgcgcca gatattgcgg gtgaatggct ttctcgctcg gtttagctaa
    28141 atcgtccagc cctttgcggg catcgcggta atcttccagc gctttatcca gaaacttgcg
    28201 atcggttttt tcttccacca gtggcagcaa tgcgcgcagg gttgatttga tatcgccgac
    28261 cagcgccata tctaccttgc tatgcgcgcc gatgctggct ggattgatat caatctgaat
    28321 aattttggca tcggtcgggt agaacgcacg gtaggggaac tgagtaccga gtagcactaa
    28381 cgtgtcggcg ttcatcatgg tatggaaacc tgacgagaat ccgattaacc cggtcattcc
    28441 aacatcatac ggattatcgt attcgacatg ctctttaccg cgtagggcat gaacaatagg
    28501 tgctttaatt ttcccggcaa actcaactaa ctctttatgc gcccccgcgc agccgctgcc
    28561 acacatcagg gcgatattgc tggaataacg cagcagttgc gccagtttgc gtaactcttc
    28621 ttcttccggc gtcacgactg gctgtggcgc atgataccag tgcgtggttg ccccttctgg
    28681 cgcaggtttt aacgccacgt cgcccggtaa cacaacaacg gaaacgccac ggttaagcac
    28741 cgctttgcgc atagcaattg ccagcacttg tgggatctgc tccgggctgg aaaccagctc
    28801 gcaatagtga ctacattcgc ggaatagctc ttgtgggtgg gtttcctgga aatagccgct
    28861 gccaatttcg ctggagggaa tatgagcggc aatcgccagt accggaacgt tattgcggtg
    28921 gcaatcgaat aggccgttga tcaagtgcag gttgcctggg ccgcacgatc cggcacagac
    28981 cgccagttct cctgaaagtt gtgcttcagc gccagcggca aacgccgcca cttcttcatg
    29041 gcgggtggac atccactcga tggtgcccat gcgattaaga ctgtcgctaa gaccgttcag
    29101 agagtcgccc gtgactcccc agatgcgttt cacgcctgcc gattcgagtg ttttggcgat
    29161 ataagctgca accgtttgtt tcatggttct ccatctcctg aatgtgataa cggtaacaag
    29221 tttagttcat ctgacggagg gggaagggat gggagagaaa ggaggcacta acggttaaat
    29281 agcccgatga aaggaatatc atcgggcata aggcgattat gcgagaacca aatccccctg
    29341 cggatggcag gagcaggcca gtacgtaacc ttcagcgatt tcggcgtcgg tcagcgtcat
    29401 tgtactgctc accgtatatt caccggaaac cacttttgtc ttacagcagc cgcaaacacc
    29461 cgcacggcag gcagcgacaa ccggaacgtt attgctttcc agcgcctcca gtagcgtggt
    29521 gccaaccggg gcgtaaaatt ctcgtgccgg ttgcagtttg gtgaatttca gaccgctggt
    29581 cgccgcttct gctactgggg tgaagaattt ctctttaaag aaacgcgtca cgccgagcgc
    29641 tttcacttcc tgctctaccc aatccatata cggagccggg ccgcaggtca tcacggtacg
    29701 tgaagctaag tcaggtacac ctgccagcag ttcgcgagtg agacgaccag cgataaagcc
    29761 ttcggtaacg ttattttctg ccaccagcgt taccggatag ttacgccact catcggcgaa
    29821 aataacatcc tgcggcgtac gcacgttgta gatcacccgc acatcggcct gtggacggtt
    29881 cttcgcaagc cagcgacgca tcgacataat cggcgtgacg ccgcagcctg ccgccagcaa
    29941 caggaattta tcttctgctt tatcgtcgca ggtaaattcc cccatcgcgt ccgaaagcca
    30001 gagataatca ccgcgtttta catcgcgcgt cagccactgg gagccgacac cgtcatcaat
    30061 ccgccgcacg gtcagggtga tatattcact cacgcctggc gtggaggaaa tggtgtaagc
    30121 acgcagcgtt tccgctgagt ttcgcacgct gaccagtgca tattgcccgg cgcgatatgg
    30181 gtagtgatcg tggcaaatca gggaaatcgt ccacacatcc ggcgtttctt gcgtaatgtg
    30241 atgaacctgc atccgccacg ggcattgatt cgttggcatc gtcatcgaca aactccttac
    30301 gcgctcaaca gttgcttcat gtcttcttca acagtggtga tagaacgcag gccgaatttc
    30361 tcgttcagca ccgccagcag gtctggtgtc aggaaaccag gtgcagtcgg gccggtgacg
    30421 atatttttca cgcccagaga aagcagcgtc agcagaatga caatcgcttt ctgttcaaac
    30481 caggagagca ccagcgacag cggcagatcg ttcacaccac agcccagttt ctctgccaga
    30541 gtgacagcca gaataattgc tgagtaagca tcgttacatt gacctgcatc taccagacgc
    30601 ggcagacctt cgatatcgcc aaactcaagt ttgttaaagc gatatttacc acaggcgagg
    30661 gtcaggatca ggcagtcatc cggcacgctg gtggcgaaat cggtgaagta gtggcgctcg
    30721 ccgcgtgcgc cgtcacagcc accaagcagg aagatatgac gcagtttttc acggctaacc
    30781 agatcaatca gcgtatcggc tgcaccaagc agcgtctggc gaccaaagcc cacggtgatc
    30841 aggtgaggaa tttcgctgta cgggaagccc gccatttgtt gcgcctgggc gatgaccgca
    30901 gagaaatctt cgccatccag atgacgcacg ccaggccagc caacgatgct gcgggtccag
    30961 atacgatcgt cataagcgcc tacggttggg tcgatgatgc agttcgaggt catcacgatg
    31021 gggccaggga aacgagcgaa ctccacttgc tgattctgcc agccgctgcc gtagttaccg
    31081 accagatgct tgaatttacg cagctccggg tagccatgcg caggcagcat ttcgccgtgg
    31141 gtgtagacat taacgcccgt gccttcggtc tgttccagca ggttgtagag atctttgaga
    31201 tcgtgaccgg aaatcagaat gcatttaccc gccgtcgctt tgacgttgac ctgggttggc
    31261 gtcgggtgac cgtatttacc ggtttcgcct gcatccagaa tgctcatcac tttgaagttc
    31321 atctggccga tttccattga acactcaaga agcgcgttca tatcggcagg ccaggtcccc
    31381 agccacgcca tgattttatg gtactgggca taaatatcgt tgtcgtattg accgagaaca
    31441 tgcgcgtgtt ccatataggc cgccgcacct ttcaggccat acaggcacag cagacgcagg
    31501 ccgagaatgt tttcgccaat cgccgcttta tctttgttag gggtaaattc tgctgcctga
    31561 cgttgcagct cgccgagatc atcgctcacc agttgcaggt cagccatcgg gttatcgacg
    31621 cgcgcgttgg catctacagc caggcattgt gctttcagcg cctcgcgcaa ggcaatcgct
    31681 tcacgagcgt agccgacaat acgcggagaa tcgaagttaa cgttggtcag ggttgagaaa
    31741 aaggcacgtg gcgcgaagct gtcaacatcg tggttgatga tgccgtattc acgcgctttt
    31801 accgcccagg cagaaagccc ttgcagcgcc gcgatgagta aatcctgaag gtcagaagtt
    31861 tccgccgttt taccacacat cccctgcgcg tatgagcagc cgtttcctgc cggagtacgg
    31921 atagtttgtt cacattgcac acaaaacatg atcacacctt ttaaagttat atttaatata
    31981 catgtttaag gttaagacgc ttaacgcggg gataaaaggg atttttcatg caactttaag
    32041 ggagattgat ttagcgcaat tttggcggca gggatctacc gccagagagg tattacgcag
    32101 agaaaaaggc gatgaggatc ggcactaaca ggctaagaat aaaaccgtga acaattgccg
    32161 ccgggaccat atccagcccg ccagtacgtt gaagaacggg cagggtgaaa tccattgatg
    32221 tggcaccgca taagcccagt gcagtagagc ggctgcggcg aatcagccca gggatcaaca
    32281 taatagcaat cagttcacgg gccagatcat taaaaaacgc cgcgctcccg attaccggac
    32341 caaaagattc ggtcaataaa ataccggaaa gagaatacca gccgaaaccg gaggccattg
    32401 ccagcgcggt attgatgggg agatcaagaa taaaggcgtt aattaaacca ccaattaatg
    32461 aactgacaac caccaccacg gcgacaatca ttccccggcg attaaggaca atctgcttta
    32521 aggtcatgcc attattgcgc aactgaatac caacgaggaa aagtagcaaa attaacgtgt
    32581 attcactggc ttcggtcgcg tgttgtaaga aagccagtcc actcagacca atggcaaaac
    32641 caatcactac tacgccgcac agttttagcg actccagcgc catcgcaata cgcgacggga
    32701 gtttttcttg ctgatggtgg ttgcgccacg gcaggcctcg ctccagccac atcagggcgg
    32761 caatattaca cagtaaaata acggtaatac tgacggcaga ataatgcaga atcgccaaca
    32821 ggttactggc gaggttatcg agaaacgcca gactgatacc cataaaaaag agaataaggt
    32881 aaaccatcca gcttaatagc tgattaataa cttttaacgc agcttgttgg cgaagcggaa
    32941 tgaggtaacc cacaatcagg ggaaccagaa tgattaacag cccagaaaac atgaaaatcc
    33001 agtccttgca aagaagaagt cgaaatgcgc gatgacacac tactgaaagc ggaaggacga
    33061 gtaaagttgc aattaaaagg aaatgttatg catcagaggg ggatttttaa tcctatctga
    33121 gtgtttagca ttgttgcgct cctttcatgt gcaatgccat ataaagccac tggcggaggg
    33181 agcgcgcagg gggcagccag tcgccgccgc cccc
//
LOCUS       DADLSB010000029        31501 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.029, whole genome shotgun sequence.
ACCESSION   DADLSB010000029 DADLSB010000000
VERSION     DADLSB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.029"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..128)
                     /locus_tag="JN331_001348"
     CDS             complement(<1..128)
                     /locus_tag="JN331_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7259536.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLL"
     gene            341..1804
                     /gene="bepA"
                     /locus_tag="JN331_001349"
     CDS             341..1804
                     /gene="bepA"
                     /locus_tag="JN331_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY7259537.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKSYTKM"
     gene            1825..2184
                     /gene="arsC"
                     /locus_tag="JN331_001350"
     CDS             1825..2184
                     /gene="arsC"
                     /locus_tag="JN331_001350"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY7259538.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2317..3063)
                     /gene="hda"
                     /locus_tag="JN331_001351"
     CDS             complement(2317..3063)
                     /gene="hda"
                     /locus_tag="JN331_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY7259539.1"
                     /translation="MVNFSRFCEILVEVSLNTSAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(3113..4402)
                     /gene="uraA"
                     /locus_tag="JN331_001352"
     CDS             complement(3113..4402)
                     /gene="uraA"
                     /locus_tag="JN331_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY7259540.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(4488..5114)
                     /gene="upp"
                     /locus_tag="JN331_001353"
     CDS             complement(4488..5114)
                     /gene="upp"
                     /locus_tag="JN331_001353"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY7259541.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            5438..6475
                     /gene="purM"
                     /locus_tag="JN331_001354"
     CDS             5438..6475
                     /gene="purM"
                     /locus_tag="JN331_001354"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY7259542.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKCHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            6475..7113
                     /gene="purN"
                     /locus_tag="JN331_001355"
     CDS             6475..7113
                     /gene="purN"
                     /locus_tag="JN331_001355"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY7259543.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            7285..9351
                     /gene="ppk1"
                     /locus_tag="JN331_001356"
     CDS             7285..9351
                     /gene="ppk1"
                     /locus_tag="JN331_001356"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY7259544.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            9356..10897
                     /gene="ppx"
                     /locus_tag="JN331_001357"
     CDS             9356..10897
                     /gene="ppx"
                     /locus_tag="JN331_001357"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY7259545.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEDAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(10936..13179)
                     /gene="pdeF"
                     /locus_tag="JN331_001358"
     CDS             complement(10936..13179)
                     /gene="pdeF"
                     /locus_tag="JN331_001358"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY7259546.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSLIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(13361..13513)
                     /locus_tag="JN331_001359"
     CDS             complement(13361..13513)
                     /locus_tag="JN331_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259547.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            13531..13722
                     /locus_tag="JN331_001360"
     CDS             13531..13722
                     /locus_tag="JN331_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY7259548.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            14033..14551
                     /locus_tag="JN331_001361"
     CDS             14033..14551
                     /locus_tag="JN331_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7259549.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            14567..15106
                     /locus_tag="JN331_001362"
     CDS             14567..15106
                     /locus_tag="JN331_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259550.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15201..16778)
                     /gene="guaA"
                     /locus_tag="JN331_001363"
     CDS             complement(15201..16778)
                     /gene="guaA"
                     /locus_tag="JN331_001363"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY7259551.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16847..18313)
                     /gene="guaB"
                     /locus_tag="JN331_001364"
     CDS             complement(16847..18313)
                     /gene="guaB"
                     /locus_tag="JN331_001364"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY7259552.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            18475..19845
                     /gene="xseA"
                     /locus_tag="JN331_001365"
     CDS             18475..19845
                     /gene="xseA"
                     /locus_tag="JN331_001365"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY7259553.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(19842..20057)
                     /locus_tag="JN331_001366"
     CDS             complement(19842..20057)
                     /locus_tag="JN331_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259554.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(20127..21599)
                     /gene="der"
                     /locus_tag="JN331_001367"
     CDS             complement(20127..21599)
                     /gene="der"
                     /locus_tag="JN331_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY7259555.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(21717..22895)
                     /gene="bamB"
                     /locus_tag="JN331_001368"
     CDS             complement(21717..22895)
                     /gene="bamB"
                     /locus_tag="JN331_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY7259556.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(22906..23526)
                     /locus_tag="JN331_001369"
     CDS             complement(22906..23526)
                     /locus_tag="JN331_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY7259557.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(23544..24818)
                     /gene="hisS"
                     /locus_tag="JN331_001370"
     CDS             complement(23544..24818)
                     /gene="hisS"
                     /locus_tag="JN331_001370"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY7259558.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKQLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(24929..26047)
                     /gene="ispG"
                     /locus_tag="JN331_001371"
                     /gene_synonym="gcpE"
     CDS             complement(24929..26047)
                     /gene="ispG"
                     /locus_tag="JN331_001371"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY7259559.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(26074..27087)
                     /gene="rodZ"
                     /locus_tag="JN331_001372"
     CDS             complement(26074..27087)
                     /gene="rodZ"
                     /locus_tag="JN331_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY7259560.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(27372..28526)
                     /gene="trmG/rlmN"
                     /locus_tag="JN331_001373"
     CDS             complement(27372..28526)
                     /gene="trmG/rlmN"
                     /locus_tag="JN331_001373"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY7259561.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(28676..29107)
                     /gene="ndk"
                     /locus_tag="JN331_001374"
     CDS             complement(28676..29107)
                     /gene="ndk"
                     /locus_tag="JN331_001374"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY7259562.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(29256..31379)
                     /gene="pbpC"
                     /locus_tag="JN331_001375"
                     /pseudo
     CDS             complement(29256..31379)
                     /gene="pbpC"
                     /locus_tag="JN331_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708358.1"
                     /note="penicillin-binding protein 1C; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
BASE COUNT         7933 a         8369 c         7788 g         7411 t
ORIGIN      
        1 agcaacggag caagcaggcc actaaagaaa aagataatgc caaatccggc aactaaaata
       61 accagcaagg caatcgcttc cgggtcgcta aaacggcggc gataccattg catcaacatt
      121 tcgagcataa agaccttccc tgaacctcaa gagcgggatt gcgatccgca attgtatcga
      181 aatgtcacaa aaaagacttc gctttttatg acggattcag gaaactgaaa agtcatttga
      241 gtgggctaat cttcgccgtt acactcaaag gcggcgaggt gggaacgata tttcacagta
      301 tcggtcaaat gactatcttc agaaatacag gatagaggtt atgttcaggc agttgaaaaa
      361 aaacctggtt gcaaccctca ttgctgctat gaccattggt caggtagccc cggcgtttgc
      421 cgacagcgca gacaccttgc cggatatggg aacctccgca ggaagcacgc tttccattgg
      481 tcaggaaatg cagatgggcg actattatgt ccgccagcta cgcggcagcg cgccgttaat
      541 taatgacccg ctgttaacgc aatatattaa ttcgctgggg atgcgtctgg tttcgcatgc
      601 caattcggtt aagacaccgt ttcatttttt tctgatcaac aacgacgaaa ttaacgcctt
      661 tgctttcttt ggcggcaacg tggtgctgca ctctgccctg ttccgttatt ccgataacga
      721 aagtcaactg gcttcagtta tggcgcacga aatctcccac gtcacccaac gtcacctggc
      781 gcgagcgatg gaagatcaac aacgcagcgc gccgctgacc tgggtcggcg cgttaggttc
      841 tattttactg gcgatggcca gtccgcaggc ggggatggcg gcgctgaccg gtacactggc
      901 gggaacgcgc caggggatga tcagtttcac ccagcaaaat gaacaggaag cggaccgcat
      961 tggtattcag gtgctgcaac gctcgggatt cgatccacag gcgatgccga ccttcctgga
     1021 aaaattgctc gatcaggcgc gttactcctc gcgcccgccg gaaattttat tgactcaccc
     1081 gttgccggaa agtcgtctgg cagatgcccg caaccgtgct aatcagatgc gcccgatggt
     1141 ggtgcagtcg tcggaagatt tctatctggc gaaagcgcgc acactgggga tgtataattc
     1201 cggacgtaac cagctcacca gtgatttgct ggatgaatgg gcgaaaggaa acgttcgtca
     1261 gcaacgagcg gcgcaatatg gtcgtgcttt acaggcgatg gaagccaata aatacgacga
     1321 ggcgcgtaaa acgctgcaac cgttactggc ggcagaacct ggcaacgcat ggtatctcga
     1381 tctggctacc gatatcgatc ttgggcaaaa caaagccaat gaggcaatca atcgcctgaa
     1441 aaatgcccgc gatttacgca ccaatccggt gttgcagctc aacctggcga acgcttatct
     1501 gcaaggcggt caaccacaag aagcggccaa tattctgaat cgctacacct ttaataataa
     1561 agatgacagc aacggctggg atttgctggc acaggcggaa gccgcgctaa ataaccgcga
     1621 tcaggagctg gctgcgcgag cagaaggtta tgcgctcgcc ggacgactcg atcaggccat
     1681 ttcgctgttg agtagcgcca gttcgcaggt gaaattaggc agcctgcaac aagcgcgtta
     1741 cgatgcgcgc atcgaccagt tgcgccagct gcaggaacgc tttaagtctt ataccaagat
     1801 gtaattcgca ttaaaggaag aacaatgacc aaacaggtaa aaatctacca taacccacgc
     1861 tgttcaaaga gccgggaaac gctgaacctg ctgaaagaaa acggcgtgga gccagaagtg
     1921 gtgctctacc ttgagacacc cgccgatgcg gcaacgctgc gcgatttgct gaaaatgctg
     1981 gggatgaata gcgcccgtga attgatgcgc cagaaagagg atctttataa agaactaaac
     2041 ctggctgaca gttcgcttag cgaagaggcg ctgattcagg caatggtgga taatccgaag
     2101 ctgatggagc gcccgattgt ggtcgcgaat ggcaaagcgc ggattggtcg tccaccggag
     2161 caggtactgg aaatcgtggg ttaagatgtg ctccatcgct agttgaagca cattgccgga
     2221 tgcgacgctt gccacgtctt atccggccta cgaatactat ggattaactt cgcgtaggtc
     2281 aggcgttcgc gccgcatccg acaataaaca ccttatctac aacttcagaa tttctttcac
     2341 aaacggaatg gtcagcttac gttgcgcggt aatcgacgca cgatccaact gatccaacgt
     2401 cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc ccacatcttc
     2461 cggcagttca aaaccacgca aacgcgcgcg taactgtagc gcctgcaact tatcttcatc
     2521 agaaagtggc tgcaatttgt agatctgccc ccagtcgaga cgcgacgcga gatccggtaa
     2581 tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaaccgtg ttttgcccga
     2641 ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat cgcctgcaat
     2701 acactcaatg ttgtcgatac agaccagcga caaatgctcc ataccgtcga gcacttccgg
     2761 aacaaaccag gtgcgtttat ccagcgggac atagcccacc gcatcgccac gctgcgacaa
     2821 ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg cccagagata
     2881 gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcggccagta aagaggagtt
     2941 atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg gcaaagagag
     3001 ctgtgccgat gtgttcagag atacctcaac caggatttca caaaatcgcg agaagtttac
     3061 cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta cattatttgt
     3121 ctgttatatc cgcgtcttct gcgtccagca ccacttcttc agggcgcagc acactgatca
     3181 gtttgaaaat caaacttaag ccgataccga cgatggtcgc cagcgccata ccttttaact
     3241 ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat gatcaaaatc acggaagtca
     3301 ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc gatcaaaaca cgaataccgg
     3361 aagcaccaat gacaccataa agcagcagcg aaacgccgcc catcaccggc aatgggatca
     3421 tctggatagc ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa atcgccgccc
     3481 cgccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg ttttctccgt
     3541 aagtggtatt tggcgtagag ccaaagaagc cggagatcac ggtcgacagg ccgttagcaa
     3601 acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata ttagccgtta
     3661 ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggcaga atagtcagaa
     3721 tggcaaacca ctcgaagcgc ggcgtataga gggttggcag tgcaaaccag tgagcattaa
     3781 taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtacccc accagcacgc
     3841 caattaaaat cgggataatt gccaggaaac cacgaaacag cacggaacct aaaaccgtga
     3901 ccgccagggt ggtaatagag atgatgatgg ttttggagtt tggcgtttgc ccttcagccg
     3961 ggagtaaacc cgccataccg gcagctacgc ccgccagctc cagaccgatg acggcaacga
     4021 ttgcgcccat tgccgcaggt ggaaacagca cgtccaacca gccggtcccc gctttcttca
     4081 cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc agcgcgactt
     4141 cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg gaaccaagat
     4201 aagccggaat tttcccttta cagatgaaga gatacagcag cgttccaata ccgttaaata
     4261 acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt gcaccaaaca
     4321 tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt ctttcactca
     4381 ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata ggcgctttac
     4441 tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta tttcgtacca
     4501 aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc gttcagtccc
     4561 tggtcaatcg atgcagtata cagttcgacg tccgggtgcg ctttttccag cgcagcgata
     4621 ccttctggcg cagcgaccag caccagaact ttgatgctgc tgcagcccgc ttttttcagc
     4681 aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc
     4741 atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc cagcgtttct
     4801 tcattacggt acataccgac aacgctgatg cgcgcgctcg gaacgttttc cagcacaccg
     4861 tccatcatac caagacccgc acgcagaatt ggcacaacgg taattttctt acctttgatc
     4921 tggtcgattt ctaccgggcc gttccagcct tcgatagtta ctttttccgt ttcgaggtcg
     4981 gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg aaagcgcttg
     5041 gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg gtgtttgact
     5101 tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaatc gacggattat
     5161 acctcctttc ttcaaggcgg caatattatt ttcgttgact ttagtcaaaa tgataacagt
     5221 ttgagataaa gttattttat attcaaatgg ttatgaaaga agattattcc atccgaaaac
     5281 taacctttgc cctggcacaa gtcttctttc gccgcgcgcc cggggaaaag acgtgcaaaa
     5341 aggttgtgta aaacagtctc gcaaacgttt gctttccctg ttagaattgc gccgaatttt
     5401 atttttctac cgcaagtaac gcgtggggac ccaagcagtg accgataaaa cctctcttag
     5461 ctacaaagat gccggtgttg atattgacgc gggtaatgct ctggttggaa gaatcaaagg
     5521 cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt ctgggcggct tcggtgcact
     5581 gtgtgcattg ccgcaaaaat atcgtgaacc cgtgctggtt tccggcactg acggcgtagg
     5641 taccaagctg cgtctggcaa tggacttaaa atgtcacgac accattggta ttgatctggt
     5701 cgccatgtgc gttaatgacc tggtggtgca aggtgcggaa ccgctgtttt tcctcgacta
     5761 ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg gtgatcagcg gcatcgcgga
     5821 aggttgtctg caatcgggct gttctctggt gggtggcgaa acggcagaaa tgccggggat
     5881 gtatcacggt gaagattacg atgtcgcggg tttctgcgtg ggcgtggtag aaaaatcaga
     5941 aatcatcgac ggctctaaag tcagcgacgg cgatgtgctg attgcactcg gttccagcgg
     6001 tccgcactcg aacggctatt cgctggtgcg caaaattctt gaagtcagcg gttgtgatcc
     6061 gcaaaccacc gaacttgatg gtaagccatt agccgatcat ctgctggcac cgacccgcat
     6121 ttacgtgaag tcagtgctgg agttgattga aaaggtcgat gtgcatgcca ttgcgcacct
     6181 gaccggcggc ggcttctggg aaaacattcc gcgcgtattg ccagataata cccaggcagt
     6241 gattgatgaa tcttcctggc agtggccgga agtgttcaac tggctgcaaa cggcaggtaa
     6301 cgttgagcgc catgaaatgt atcgcacctt caactgcggc gtcgggatga ttatcgccct
     6361 gcctgctccg gaagtggaca aagccctcgc cctgctaaat gccaacggtg aaaacgcgtg
     6421 gaaaatcggt atcatcaaag cctctgattc cgaacaacgc gtggttatcg aataatgaat
     6481 attgtggtgc ttatttccgg caacggaagt aatttacagg caattattga cgcctgtaaa
     6541 accaacaaaa ttaaaggcac cgtacgggca gttttcagca ataaggccga cgcgttcggc
     6601 cttgaacgcg cccgccaggc gggtattgca acgcatacgc tcatcgccag cgcgtttgac
     6661 agtcgtgaag cctatgaccg ggagttgatt catgaaatcg acatgtacgc acccgatgtg
     6721 gtcgtgctgg ctggttttat gcgcattctc agcccggcgt ttgtctccca ctatgccggg
     6781 cgtttgctga acattcaccc ttctctgctg ccgaaatatc ccggattaca cacccatcgt
     6841 caggcgctgg aaaatggcga tgaagagcac ggtacatcgg tgcatttcgt caccgatgaa
     6901 ctggacggtg gcccggttat tttacaggcg aaagtcccgg tatttgctgg tgatacggaa
     6961 gatgacatca ccgcccgcgt gcaaacccag gaacacgcta tttatccact ggtgattagc
     7021 tggtttgccg atggtcgtct gaaaatgcac gaaaacgccg cgtggctgga tggtcaacgt
     7081 ctgccgccgc agggctacgc tgccgacgag taatgccccc gtagttaaag cgccagctct
     7141 gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt tttccccctt
     7201 ttctggcata gttggacatc tgccaatatt gctcgccata atatccaggc agtgtcccgt
     7261 gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc tatacatcga aaaagagctc
     7321 agttggttat cgttcaatga acgcgtgctt caggaagcgg cggacaaatc taacccgctg
     7381 attgaaagga tgcgtttcct ggggatctat tccaataacc ttgatgagtt ctataaagtc
     7441 cgcttcgctg aactgaagcg acgcatcatt attagcgaag aacaaggctc caacgctcat
     7501 tcccgccatt tactgggcaa aattcagtcc cgggtgctga aagccgatca ggaattcgac
     7561 ggcctctaca acgagctatt gctggagatg gcgcgcaacc agatcttcct gattaatgaa
     7621 cgccagctct ccgtcaatca acaaaactgg ctgcgtcatt attttaagca gtatctgcgt
     7681 cagcacatta cgccgatttt aatcaatcct gacactgact tagtgcagtt cctgaaagat
     7741 gattacacct atctggcggt ggaaattatc cgtagcgata gcatccgtta cgcgctgctg
     7801 gagatcccat cagataaagt gccgcgcttt gtgaatttac cgccagaagc gccgcgtcga
     7861 cgcaagccga tgattcttct ggataacatt ctgcgttact gccttgatga tattttcaaa
     7921 ggcttctttg attatgacgc gctgaatgcc tattcaatga agatgacccg cgatgccgaa
     7981 tacgatttag tgcatgagat ggaagccagc ctgatggagt tgatgtcttc cagtctcaag
     8041 cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc caatgcgctg
     8101 gttgaagtgt tacgcgaaaa actgactatt tcccgctacg actccatcgt ccccggcggt
     8161 cgttatcata attttaaaga ctttattaat ttccccaatg tcggcaaagc caatctggtg
     8221 aacaaaccac tgccgcgttt acgccatatt tggtttgata aagcccagtt ccgcaatggt
     8281 tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc cttatcacac ctttgagcat
     8341 gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg tactggcgat taaaattaac
     8401 atttaccgcg tggcgaaaga ttcacgcatc atcgactcga tgatccacgc cgcacataac
     8461 ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt tcgacgaaga agccaacatt
     8521 cactgggcga agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc gccggggctg
     8581 aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt ggtccgttac
     8641 gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac tgactattcg
     8701 ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc gggtatttaa ctttattgaa
     8761 aacccatacc gcccggtgac atttgattat ttaatggtgt caccgcaaaa ctctcgccgt
     8821 ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct gcccagtggt
     8881 atcaccctga agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct gtatgcggcc
     8941 tccagctccg gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct gatccccaat
     9001 ctggaaggca ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta ccttgaacat
     9061 gaccgggttt atatttttga aaatggcggc gataaaaagg tctacctttc ttccgccgac
     9121 tggatgacgc gcaatattga ttatcgtatt gaagtggcga caccgctgct cgatccgcgc
     9181 ctgaagcagc gggtgctgga catcatcgac atattgttca gcgatacggt caaagcacgt
     9241 tatatcgata aagaactcag taatcgctac gttccccgcg gcaatcgccg caaagtacgg
     9301 gcgcagttgg cgatttacga ctacatcaaa tcactcgaac aacctgaata accctatgcc
     9361 aatacacgat aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg gctcaaacag
     9421 ttttcacatg gtcatagccc gtgtggtaga tggtgccatg cagattattg gccgcctgaa
     9481 acagcgggtg catctggcgg acggcctggg gccagataat atgttgagtg aagatgcaat
     9541 gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt tttctcctgc
     9601 cagcgtctgt atagtcggca cccatacgct gcgtcaggcg ctgaacgcca ctgactttct
     9661 gaaacgcgcg gaaaaggtca ttccctaccc gattgaaatt atttccggta atgaagaagc
     9721 ccgtctgatt tttatgggcg tggaacatac ccaaccggaa aaaggtcgca aactggttat
     9781 tgatattggc ggcggatcta cggaactggt gattggtgaa aatttcgaac ctattctcgt
     9841 tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt tattttcctg gcggggtcat
     9901 caataaagag aattttcagc gcgctcgcat ggcggcagca caaaaactgg aaactttaac
     9961 ctggcaattc cgtattcagg gctggaacgt tgcaatgggc gcttccggta ccataaaagc
    10021 cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg ataattaccc cggaacgtct
    10081 ggaaaaactg gtaaaagaag ttttacgtca ccgtaatttc gcatcgctga gtttaccggg
    10141 tctttccgaa gagcggaaaa cagtcttcgt tcctggactg gcgattttat gtggtgtgtt
    10201 tgatgcttta gccatccgtg aactgcgcct ttctgacggg gcgttacgcg aaggcgtact
    10261 gtatgaaatg gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg ccagcagcct
    10321 cgccaaccag tatcacatag acagcgaaca ggcccgacga gtgctggata ccactatgca
    10381 aatgtacgaa cagtggcggg aacagcaacc gaagctggcg catccgcaac tggaggcgct
    10441 actgcgatgg gccgccatgc tgcatgaggt cgggttgaat atcaaccaca gcggtttgca
    10501 tcgccactcc gcttatattc tgcaaaacag tgacttgccg ggttttaatc aggaacagca
    10561 gctgatgatg gcgacactgg tgcgctatca ccgtaaagcg attaagctcg acgatctgcc
    10621 gcgctttacc ttgtttaaga agaaacagtt cctgccactg atacagctat tgcgccttgg
    10681 cgtattacta aacaatcaac gtcaggcaac caccacaccg ccaacattga cactgattac
    10741 tgatgacagt cactggacac tgcgtttccc gcatgactgg tttagtcaga atgcgctggt
    10801 actgcttgat ctggaaaagg agcaagaata ctgggaaggc gtggctggct ggcggttgaa
    10861 aattgaagaa gaaagtaccc cagaaatcgc cgcttaatgc tttgcgggcc gacgagaaat
    10921 gtcggcccgc attattcagg cactttcgcg aatgggttcg atttcattca gcgtatcaat
    10981 taacggttgc ggcttaccaa taagataacc ctgcatataa tcgatcccca aagagagcac
    11041 cgcctcgcgg atctcttcgt tttcaacgta ctctgccact acccgcattt tcttcattcg
    11101 cgccagatgg caaatcgacg ccacaatctg ataatccaga ctatttgaca caatattgcg
    11161 gataaagctg ccgtcaattt taagcagatc ggcattcaca tttttaagcc gtgcatagct
    11221 ggcgtagccg gtgccgaaat catcaatcgc aatctggcag cctaattcct gaagatgctg
    11281 caaggtgatt tgcgcctgct taacattggt cagagcatta ctttcggtga cttcaaaaat
    11341 aagttgccac gcttcaatct gatatttagc cagcaactga ctgacttcaa caggaaaacg
    11401 agcctgacat accgaggttg gagacagatt aatagcaaaa cggtgagcgg gcatcttcgc
    11461 tctgttttca gccataaatt gcagcgtatg ctcaatgacc cacatgtcga tactcgacga
    11521 taaaccaaat tcgtgcgcga ccggcaagaa gctatcgggg ccgatcagtt catcattctc
    11581 acctttcatg cgcagaagaa tttcatggta aacatcacca cgcataccgg taatcggctg
    11641 ggccatcagg aaaaaatggt tgtgttccag cgcctgctgt agtcgattca tcatcgcgac
    11701 tttatctttc aattcgcgtt gcaaatacat tgccccgcaa cgctgcatat tttccggggc
    11761 gttggtcacg atggaaagtt cggcgaccgt atttagctct cccagcagca ggtagatatg
    11821 attcactggc gagcgcacat agcagtaact gacgccaatc tgcggttgca tcggcatccc
    11881 atcccaaaag aaacgaaatt gcttgagatg actatccagt gcggtaatgc gctcctggtg
    11941 cgattctgta ttcagtcgca gcgcgagatc gttacccgaa agctgataaa catcttcacc
    12001 cggttccagc aagggtgaca gccagtgaga aagtttttgc ttgtattgaa tccgcagcat
    12061 gatgccatag ttcttaacca gcatttccat gccagggatg cgtaaataac aaagcgcaga
    12121 ccaaggggca tcacgcaacg cgcgattcag ggcgcgaaca tttggcagat gaaccaccgg
    12181 gtccacatac gcaagccgct gaatgcgtct gactaccgct cgctgacgag ttgccagtac
    12241 agccatataa ttgacaatca aagagaatac cagataactg gaggaggtta tggtcagctg
    12301 cgtggtatag ccaggataaa tgggaatgta attttgatag ctgtggatgc tgatcatcaa
    12361 cacgaccgcc cagagcagcg aaatcagctt ataaccatag cgcattgctc cccacatcat
    12421 caggggcagc aataatgaca aggtataatt ggtgctaaaa attgtgcttt tttcatttaa
    12481 cggcatgcat aacagcaaca ataaagcacc taatgccagt agccagagcg cgaactcttt
    12541 tttggtgact ttggcatcaa cctgctgttt taattgcgaa taatagctac gtaaataaaa
    12601 tggatttcgc actacccgaa tgatgaagta gcacagcggg acaccgatca gattacccac
    12661 cagtaaggcc tgataattga ttaaagtccc gaggttaaaa ggcatgacac cgaccagatt
    12721 ttctctgctc gccagtaatc ctacaaatgc agcaaactgg aaaagtatca gaaacagcgt
    12781 tgcaggaaac actatctgcc agaatatacg ctgggaaatt aaacgggtat caccatgtga
    12841 aacgttgtta cgccggggag taaagaccct gtaaccgccc caacagagaa tgataatgat
    12901 aataaaatta gccgttaatg agagcgtttc gtaaaaaccc agctctgcat atttgcggac
    12961 aaaaatccct aaggtgatcc ctggcaatgc cgcccagctg aaaaacatca tcatgctgat
    13021 catcagtgcc aaaggcagat aaaagagaaa aacctcaccg gaagaaatat gcgcgaaagt
    13081 attaatgtgg gcgaaaatgg gcaagattaa agaaggcagg aacagcggaa gcccccacca
    13141 tttatcacgt atttttatat aagttgcatt cagtttcata gatgctcagc agaatccccc
    13201 acatcctgaa ggaggtgtat tcagacaggc atcccacctg acttcgaatg atgattattc
    13261 atcactatag agagcattga ttctaagtgt catatgaaag ttccaattga tatatatcaa
    13321 acaaaataac cctgattaat gaattattac gtttatcatg ttaattcatc attattacat
    13381 catcattgta aataattaaa ttaacttcca taacattaaa atatgtatcc actgacgctt
    13441 ttttacataa cgaagaattg accattttgt cctgttgtgc cttaatgtaa gtaccgtcca
    13501 cagcgtggga catacttcaa ggaacctttt gtgagtcagg caactagtat gcgaaaacga
    13561 caccgattta acagtcgcat gacccgtatc gtactgctca tcagctttat cttcttcttt
    13621 ggccgtttta tctactcgtc cgtcggtgcc tggcagcacc atcagagcaa aaaagaagct
    13681 cagcaatcca cactctccgt cgaatcaccg gtacaacgtt agcggttacc ttctccactt
    13741 tcacagaaca taacggcact tcgctgtcgg atgcttttgc tctttgggat tatcaaagcg
    13801 gcagatattc tttcatctta aattttacgt ctttgtcctg actgatgttt atcctgtttg
    13861 gctgcgaaat aaatataaaa ttaatatata tgttgtaatg atatattttt ataaattatt
    13921 ccctgcgtga attttaataa atttaatcta tccctttata cgcaatacat ttactttcct
    13981 cttttgatga tcttaaatgt cttatttttc gtaatgtgta taacaaggaa tagtgatgaa
    14041 atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca ttcactctgg caggatgcca
    14101 gtcaaatgct gacgatcatg ccgccgatgt ttatcaaacc gatcaactga ataccaaaca
    14161 agaaactaaa accgttaata ttatttccat tcttcccgca aaagttgccg tagacaactc
    14221 ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt ggcgcagtcg ctggcggtgt
    14281 tatcggccac aacgtcggtt ctggcagcaa ttccggaacg acggcagggg cagttggcgg
    14341 cggagctgta ggcgcggcag cgggttctat ggtgaatgat aaaaccttag tggaaggtgt
    14401 ttctttaaca tataaggaag gcaccaaagt gtatacctcc acccaggtgg gtaaagagtg
    14461 ccagtttacg acaggtttag ccgttgttat taccacaacg tataacgaaa cgcgtattca
    14521 gccaaatacc aaatgtcctg aaaagagcta ataatcagga ggagtcatga agaaagtttt
    14581 tctttgcgcc atcttagcct ccttaagcta tccggctata gcctcatcat tgcaggatca
    14641 actctctgct gtcgcagaag cggaacagca aggtaaaaat gaagagcaaa ggcagcatga
    14701 cgaatgggtc gcggagcgca acagggaaat ccagcaagag aagcaacgtc gcgcaaatgc
    14761 ccaggccgcc gcgaacaaaa gagcggcaac ggcagcggca aataagaaag ctcgtcagga
    14821 taaactggac gccgaagcca ctgcggacaa aaaacgcgat caaagttatg aagatgagct
    14881 acgcagctta gagattcaga aacaaaaact ggcgctggcg aaagaagaag cccgcgttaa
    14941 gcgcgaaaac gaatttatcg atcaggaact gaagcacaaa gctgcgcaaa ccgatgtggt
    15001 gcaatctgaa gctgacgcaa acagaaatat gactgaaggc ggtcgcgatc tgatgaaaag
    15061 cgtgggcaaa gcagaagaga acaaatcgga cagctggttt aattaatcga tgttagtaac
    15121 ttcaagccta tgattcttga agataaaaaa ccctctgtag taacggaggg ttttgttcat
    15181 tcatagtgca gggatcaaaa tcattcccac tcaatggtag ctggcggctt gccgctgatg
    15241 tcatacacca cgcgggaaat accgttcact tcattgataa tgcggttgga aacgcgaccg
    15301 aggaaatcgt acggcagatg cgcccagtgt gcggtcataa agtcgatggt ttcgacagca
    15361 cgcagagaga caacccagtc atacttacga ccatcgccca ttacgccaac ggaacgtact
    15421 ggcaggaaga cggtgaacgc ctggctgact ttgtcgtaca ggtccgcttt acgcagttct
    15481 tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt cacttcaccc
    15541 agaacacgaa cgccaaggcc tggtcccggg aacggatgac ggtacagcat gtcgtacggc
    15601 aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
    15661 accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg
    15721 tgtgctttac cggttgcaga agccgcagat tcgataacgt cagggtagat ggtgccctgc
    15781 gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaatacttc aacgaaaacg
    15841 cgcccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc tgacaggaag
    15901 cgatcttctg ccggtacgtg aacaatgtta agaccaaagt gatcgccaaa catatccaga
    15961 acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca agtcaggttt
    16021 ttaccgatag cacggtgcag cagcattgcg gttacggagg aatccacacc accagagagg
    16081 ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc gagctacagc atcgtcgata
    16141 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
    16201 atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
    16261 ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac ggtgacgaag
    16321 tcggacggga tagcggtaac tttatcgccg tggctcatcc agacatcgag cagcggttta
    16381 ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca
    16441 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
    16501 gccatggtct gcatgccata gcaaacgccg aatatcggta cgcctgcttc aaagacatac
    16561 tgcggcgcac gcggactgtt ttcttcagta gtactttccg ggccgccgga aagaataatg
    16621 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca cagttcgcag
    16681 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtcc
    16741 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
    16801 gaaacagata atataaatcg cccgacataa agtcgggcga agagaatcag gagcccagac
    16861 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatacccg
    16921 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
    16981 tcagacccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
    17041 ctttataagc tacgcgacct tcgatacctt ccggcaccag tttgtcggca gcgttatcgc
    17101 tctggaaata acggtcagag gaacctttgg acatcgcgcc cagggaaccc ataccacggt
    17161 aagatttata agaacggccc tggtagagtt cgatttcacc cggagattct tcagtacccg
    17221 ccagcatgga acctaccatc accgcgcttg cgccagcggc gatagctttg gcgatgtcgc
    17281 cggagaagcg aataccacca tctgcgataa ccggaatacc ggtgccttcc agggcttcaa
    17341 ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc agtaacgata cgagtcgtac
    17401 agatagaacc agggccgata ccgactttaa ccgcgctgca accagcttct gccaaagcgc
    17461 gtgcacctgc agctgttgcc acgttgccgc cgatgatttg cagatccgga tatttagcac
    17521 gggtttcacg gatacgctgc aaaacgcctt ctgagtgacc gtgggaggag tcgatcagca
    17581 gaacgtcaac gccagcggca accagcgcgt caacacgctc ttcgttaccc gcacctgcgc
    17641 caactgcagc accaacacgc agacggcctt gctcgtcttt acaggcgttc ggtttacgtt
    17701 ccgctttctg gaagtctttc acggtgatca tgccgatcag gtggaattcg tcatcaacca
    17761 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcac
    17821 cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggttca
    17881 ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc caccagttcg ttttcttcgg
    17941 taacgaccgg atagcccgca aaaccgttac gctcggtcag ttctttcact tcgcgcagcg
    18001 tcgtggttgg cagaacagtc tgcggatcag tcaccacacc agattcgtgt tttttcacac
    18061 ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag ccgataccgc
    18121 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
    18181 gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca gcagtattcg
    18241 gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
    18301 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
    18361 tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct atccccgaag
    18421 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
    18481 ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg
    18541 cttgagcatg agatgggaca ggtttggatc agcggcgaaa tctctaattt cacacaaccg
    18601 gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg
    18661 ttccgcaaca gcaaccgccg ggtcaccttc cgtccgcagc atgggcaaca agttttagtt
    18721 cgcgccaata ttacgctcta cgagccgcgc ggcgactacc agataatcgt tgagagtatg
    18781 cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc gaagttgcag
    18841 gctgaaggtt tgttcgatca gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt
    18901 ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt
    18961 cgcgatcctt ctctaccggt gatcatctac cccacctccg ttcagggcga tgacgcgccg
    19021 gggcaaattg ttcgcgccat tgaactggcg aatcagcgca atgagtgcga cgtgttgatc
    19081 gttgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga acgcgtagcg
    19141 cgggcgattt ttgccagccg cattccggtc gtcagcgccg tcgggcatga gacggatgtg
    19201 accattgccg attttgttgc cgatctgcgt gcgccaacgc cgtctgccgc cgctgaagta
    19261 gtgagccgta atcagcaaga gttactgcgc caggtgcaat cggcccgtca acggctggag
    19321 atggcgatgg attattatct cgccaaccgt acgcgtcgct ttacgcagat tcatcatcgc
    19381 ttgcagcagc agcatccgca gctccggctg gcacgccagc aaaccatgct tgaacgcctg
    19441 caaaaacgga tgagctttgc gctggaaaat cagcttaagc gtgccgggca acagcagcag
    19501 cgattaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg cacgcaaacg
    19561 cgcattcagc aactggaata tcgtttagca gaaatcctgc gcgcacagct tagcgccacg
    19621 cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact gtcaacgctg
    19681 gcgcgtggat acagcgttac tactgctact gacggcaatg tactgaaaaa agtgaagcaa
    19741 gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg gctggataga aagtgaagta
    19801 aaaaacatcc agccggtaaa aaaatcgcgt aaaaaggttc attaagccag cacgaattca
    19861 acgcgttttt tcgatatcaa gccatgcccg tgctgacaga agtaatctac tgcaccgcag
    19921 gcttttaaca cctgcaacgg ttgatggcag tctggacaaa gagctttcat ttcaataaat
    19981 tccccgcagg atgggcaacg cgcatggccg ttatcctgat caagcacatg ctgacatttt
    20041 ggacaatgca gttccatatg aattcctccg gtagccattc cctctacatt catagaggga
    20101 atggcagata aaatacttac ggataattat ttatttttct tgatgtgctt catcagacgc
    20161 ttacgtttac gcatctgggt tggcgtcagg gtgttacgct tgttcgcata cgggttttcc
    20221 ccttctttga actgaatacg aatcggcgat cccattacgt ccagcgattt gcggaagtag
    20281 ttcatcaagt agcgcttgta ggaatcaggc aggtctttca cctgattacc gtgaatcacc
    20341 acaatcggcg ggttataacc accagcgtgg gcatatttca gcttcacacg acgaccgcgt
    20401 accagcggcg gttggtgatc ttcaacagcc atcgtcatga tgcgcgtcag catagaggtc
    20461 cccacacgac gggtggagct gtcatacgct tcacgtactg attcaaacaa gttaccaaca
    20521 ccactgccgt gcaaggcaga gataaagtgc acacgagcaa aatcgataaa gcccagacgg
    20581 aagtccagcg tttctttcac ctgctctttc acttcctggc tcaggccatc ccacttattc
    20641 accacaatga caagtgagcg cccactattg agaataaagc ccagcagcga gagatcctga
    20701 tcggaaatac cttcgcgcgc atcaatcact aacatcacca cgttggcgtc ttcaatggcc
    20761 tgcaacgttt tgattacgga gaatttctct acagcatcgg tgattttgcc gcgtttacgt
    20821 acgccagcgg tgtcaatgag cacatactca cgtccatcgc gttccattgg gatgtagatg
    20881 ctgtcacgcg tcgtgccagg catgtcgtaa acaacaacgc gctcttctcc aagaatacgg
    20941 ttagtgagtg tagacttacc tacgttcgga cgacccacaa tcgccagttt gatcggcaga
    21001 ctttgcgggt cgaagtcgtc ttcctcttct tcttcgccgt tctcttccgc ttcaaattgc
    21061 gcccagtatt cagcgtcttc gtcgacttcc tcttgcggtg cgagatcttc catccacggc
    21121 agcagcacat gctccagcag acttaatacg ccacgaccgt gagacgcggc gatcgggtag
    21181 atttcaccta aaccaagcga gtagaaatca accactgcct gatcgggatc cagaccgtca
    21241 gttttgtttg ccaccaagaa ggtcggtttt tcacgggagc gcagatgttt ggcaatcgct
    21301 tcatctgccg gcatcaggcc cgcgcgcgca tccaccataa acagtacgac gtccgcttct
    21361 tcaatcgcca gcagcgactg ttccgccatg cgggtttcta caccgtcttc tgtgccatca
    21421 atcccgccgg tatcaataca gataaactca cggccttcaa tttccgcacg accgtactta
    21481 cggtcacgag tcagacccgg gaaatccgca accagcgcat ctcgggtgcg agttagacgg
    21541 ttaaataacg tggattttcc tacgttaggg cgcccgacaa gcgcgaccac aggtaccatg
    21601 tttaaagcct catttttata aatcatcaga caacgcacgc tatattcgcg tcgttgttaa
    21661 aaacaggaaa acggcccctg tccaggagcc gttttcaaag tgaacgacag agacgattaa
    21721 cgtgtaatag agtacacggt tccgtctttt gcctggatca gcagtttgcc gtcagcggca
    21781 accggttcag tctggaaacc ggaactatca actttttgct gggcaacgaa acgaccatct
    21841 tcgacgttaa tccagtgcag ataaccttca ctgtcaccga ccaccaggtt gccattatac
    21901 agcaccggag aagtcagcag gcgatgcagc agatcgcttt gtgcccacag cgtaacgccg
    21961 ccatcaatgg tcaacgccat cacccggtca ttttgatcga ccagatagat gcgattgcca
    22021 tcgacgatga aatcattcac cgaacccagt tcgcgtttcc acataatctg accactgcgc
    22081 agatcaagcg ccgtcaggtt accattatag gccagcgcga aaacaacgcc gttaacaacg
    22141 acgggagtcg tgtcaacatc gctcagacgg tcaatttcgg tagaaccggt cgcctgggaa
    22201 atacgctgct gccaaatcat ctggccctgt tccatcagca ctgcgctgac gcgaccatta
    22261 tcgccaccca cgacggccgc accaaaagcc gttgccggcg cagactcgcc acgcaaagag
    22321 agcgaaggca tatcgaggtt aactgtccat ttgacagcgc cgtcagcttc gttcagcgct
    22381 tgtaactgac cgttactggt gtggattaac accagaccgt cgctgaccac cgggcgcgaa
    22441 agtgcttcac ccgcgacttt agtttgccat gccacagtac catcgctggt attcagcgcg
    22501 taaacctgcg ccttttcgct gccaatgtag acatgcccac cagacacggt cacaccgcca
    22561 gaaagtaatg caggctcttt agagaaccag ccatctttct cggccaggct gacagaccag
    22621 atttctttgc catcatccgc attcagcgct tttaccaaac cagcgcggtc cgctgcatag
    22681 acaacgttgt ccgccagtgc cggatgaaga ttggaataga agttgccaat gccgctacca
    22741 acggaagtgc tccacgccgt ggtcggcgta aactggtttt caacggttgg caatggggac
    22801 atctttacca catcttcttc gctgttaaac agcgaacagc cgcttaaaag ggtaacggaa
    22861 agcagtcctg gcagcagtaa tttacgcaat tgcatcgggt ccctctcaga tggacaaatt
    22921 attaattttc atctgcatca tttcgctcag tgccggagta acatcgcttt tcacgcctgc
    22981 ttcccatgca ctacgcgcac cttgcttatc acctttgctc agcaatgctt caccacgcag
    23041 gtcggcaaca atggcagccc acccttcacc tttgatggta tcaagggttt tcagcgcggc
    23101 atcagcctgc ttgagctgta cctgaacgcg agcaagacgc agatttatca cggctttgag
    23161 attttcatcg ctcgtgtctg ccagcccctg ttgtaactgg gcggcagctt tctctagttc
    23221 atttttgtca acaaattgct gcgcaagttc caaagaagcc agcgcaccat aagtattttt
    23281 attttcagca gcaaattttt ccgccgccgg gatgctatcc ggtttgcctt cgctcactgc
    23341 ggtaaccgca ttttgatagg caagagaagc ggagcgtgca gaatcaacct gatggctgtt
    23401 ccagtagcgc cagccaatca gtgcgccaac gcccaaaatc accccaacag ccagtgcttt
    23461 gccattttca gcaaaaaagc gtttaaccgc ttctacctgg tcgttttcgt tctcgtaaat
    23521 ttccacgctg tccttctcct tccttaaccc agtaacgtgc gcaaatgcgc ggctacgcta
    23581 tcctgcgcaa ctgccgtttg ctcaccagag cgcaaatcct tcactactgc tgtgccgtta
    23641 gccacttcag actcacccag caccacagca acgcgggcac cccatttatc agcacgggca
    23701 aactgtttct taaagttgcc gccgccgtgg ttggtcatca atttcacgcc cagtaattca
    23761 tcacgcagac gctcagctaa tgccatagcc gcagattgtg tatcagcacc tgaagccacc
    23821 aggtatatat cgacaacagg atcggcttta aattccggat taacggcctg tactaacaat
    23881 acaagacgtt cgaggcccat cgcaaaaccg acagccggtg ttgcacgacc gcccagttgt
    23941 tccacaagac cgtcataacg accgcctgca cacacggtgc cctgggagcc gagactgtta
    24001 gtcacccact cgaaaacggt acggttatag taatccagac cacgcaccag acgctggttt
    24061 acggtgtaag cgatccccgc gctctccagc tgtttgcaca gaccggcaaa atgctcacga
    24121 gattcctcgt ccagataatc acctaatgcc ggagcgtcgt tgagaagcgc ctgcacttcc
    24181 ggattttttg aatccagcac gcgcagcggg ttagtgtaca tgcggcgttt gcagtcttcg
    24241 tccagctttt ctttatgctg ctcaaggaat gccaccagcg catcgcggta attggcgcgt
    24301 gcttccagcg aaccaataga attcagctca agagttacgt gttcgaagat acccagcgca
    24361 cgccaccagc gggcagtgag cataatcagt tcagcgtcga tatccggacc ttgcagaccg
    24421 aaaacttcgc agcccaactg atggaactga cgataacgcc ctttctgcgg acgctcgtga
    24481 cggaacatcg gcccgatata ccacagacgc tgttcctgat tgtacagaag accatgctcg
    24541 atgccggcgc gtacacagcc cgccgtccct tcagggcgca gagtcaggct gtcgccattg
    24601 cgatcctcaa aggtgtacat ctctttttca accacgtcgg tgacttcacc aatcgcacgt
    24661 ttgaatagcg gggtctgctc tacaatcggc aagcggattt cactgtaacc gtagctgccg
    24721 agcacgtttt tcagtgtgcc ttcaatgcgc tgccagatgg ccgtttcgcc aggcaggtaa
    24781 tcgttcatgc cgcgaatggc ttgaatgttt tttgccacgt ttattctctt tctgaatata
    24841 aaaatgaacc ctcaacgctt ccctcaatgt ttcgggagcc atgcgggttc aatcatacac
    24901 gggaagcgag gcgcttccca tcacgttatt atttttcaac ctgctgaacg tcaattcgac
    24961 gcgcttcgtc cagctgactg gctttcgcac gaatgcgtgc ttccagctgg tcgatcatat
    25021 cgttgttgtc cagacggtct ttgcgcacgc catcttcata gaggccgctt ttcttgttgc
    25081 cgccggtgac gccgagtgta gaaaccagcg cctcacctgg gccattcacc acgcagccga
    25141 taatcgaaac gtccatcgga gtgatgatat cttccaggcg ttgctccagc gcgttaaccg
    25201 taccgataac atcaaattcc tgacgcgaac aggtcgggca ggcgatgaag ttaatccctc
    25261 gcgaacggat acgcagcgat ttcaaaatat cgaaaccgac tttgatctct tcgaccggat
    25321 cggccgccag cgatacgcgc agcgtgtcgc cgatgccttc cgacagcagc agacctaaac
    25381 caatggcgga ttttactgcc ccgctgcgtg caccaccggc ttcggtgatc cccagatgca
    25441 gcggctgatc gatctgtttt gccagcaaac gataagactc aacagcgagg aagacgtcag
    25501 acgctttcac gctgactttg aactgatcga agttcaggcg atcgagatga tcaacatgac
    25561 gcatggcgga ttccagcaac gcctgcggcg tcggttcgcc atacttttct tgcagatctt
    25621 tttccagcga tccggcgtta acgccaatac ggatcggaat gtttttatcg cgcgcacagt
    25681 caaccaccat gcgaatacgc tcttcattac cgatattacc agggttaata cgcagacaat
    25741 cgacgccgta ttccgctact ttcagcgcaa tgcgatagtc gaagtggatg tcagccacca
    25801 gcggcacgtt aacccgctgt ttgatgagtt tgaacgcttc tgccgcgtcc atcgtcggta
    25861 cggagacacg gacgatatca gcgccaacgc gttccagcgc cttgatttga ttgaccgttg
    25921 cttcgacatc tgtcgtacgc gtattggtca tggactgtac ggcgatggga gcaccatcgc
    25981 caatcggcac attcccaacg taaatacgtg ttgattttct acgttgaatt ggagcctggt
    26041 tatgcatgaa aaatctcccg cgttacccgt ctgttactgc gccggtgatt gttcggcatt
    26101 gagggtcaga cgcgcaacct ggttagttct gataaaacga ctcagatcga caggtttccc
    26161 ttgatactgg atctgtactg cggctggcgc accaattttc agtttatacg gtgcctggcc
    26221 ggttaaattc aaattaccgt ctttacgctg catcccgcta aacaattttt taccggtagc
    26281 atcagtgacc tccagccagc aatcggcagt aaagttcatc accagcgcat tcggatcagc
    26341 cgccggcgtg gtcacgccag cctgatcggt tggcaacggc gcagcaccat ctggcgttgt
    26401 tgctgccgtt ggtaccgggg tcgcggcggt atcaacattt gcctgcgaag gcgaaacaac
    26461 cgcattctgt tgcggatcaa cagctggtgc tggcgcagtt acggcaggtg tttgcgtgtt
    26521 ggttgcggta gtatccacag acgctggcgg cgtggacgtt gtagccgggt ctgtagttgt
    26581 cgacgtattt aacggaacac tctgcccctg ctcgctatta ctgctcagtt ccgccgaaga
    26641 ttgatcggcc atagtggtga tctcttcctg ctgagctttg tggtcttgcc accaccaggc
    26701 accgctcagg ccgataacca caaacaacac cagccaggtg aaggtcatca gccagccgtc
    26761 gcgttttttg cggcgtttac cgagggaaaa actctgcatt ggcgcaactt ttgcagcccg
    26821 aagtggagcc tgcttttcca gccctggcag cagttcttct tctggaatat gtaccagacg
    26881 cgcataagag cggatatatc cgcgcaggaa tgttgaagca agatcggcgg gtgccttatc
    26941 ttcttcaatg tcgcgtaccg tggaaacctt caggcaaagt cgctcggcaa cggcctgctg
    27001 actaagtcct agttgttcgc gagcattacg caggcgagcg ccggtagtaa gtgcttcatt
    27061 ttggtcgtgc gtggcttcag tattcattcg ctgcaggtac gtttaaatga gaattgagat
    27121 gccggtgaac catcatgctc acccacatcg cgaaacatcc gttaagttaa ccgttatcat
    27181 acagtataag accgtctgtg tgctcgtgac aaagcccaca ttcactgagg ctaacttact
    27241 gttgcatcgt tacatactgc cttaaagtca gcaaaaacgc accgttaata ttgaccagac
    27301 aattgcaact taatacttca ttcataaaag attacgccac ggtacataaa gtaaccgtgg
    27361 cgtaatggct atcagaccgc tttaatatcg atggcttcac cctgcatccg tttacgcaga
    27421 gtacgtttcg tacggtcgat aacatcgccc gccaactgac cacaggcagc atcgatatca
    27481 tcaccacgcg ttttacgcac aatagtggtg aaaccgtagc tcatcagcac ttttgagaaa
    27541 cggtcgatac ggctgttcga actgcgtcca tacggcgcgc ccgggaacgg gttccacggg
    27601 atcaggttga tcttacacgg cgtatctttc agcagttccg ccagttggtg cgcgtgttca
    27661 gtgccgtcgt taacgtggtc aagcatcacg tattcaatag tgactcggcc ctgattggcg
    27721 ttggatttct ccagataacg gcgcaccgca gcaaggaacg tttcgatatt gtactttttg
    27781 ttgatcggca caatttcgtc acgaatttcg tcgttcggcg cgtgcaggga aattgccagt
    27841 gcaacgtcga tcatatcgcc cagtttatcc agcgccggaa ctacaccgga agtggaaagc
    27901 gtcacgcgac gtttagacag gccaaaaccg aaatcatcaa gcatgatttc catcgccgga
    27961 acgacgttgt tcaggttgag cagcggctcg cccatgccca tcatcacgac gttagtgatt
    28021 gggcgctgac cggtgacttt tgctgcgccg acgattttcg ccgcacgcca cacctggcca
    28081 ataatttccg atacccgcag gttgcggtta aagccctgct gggcggtgga acagaattta
    28141 cactccagcg cacaccccac ctgcgaagag acgcagagcg tggcacggtc gtcttccggg
    28201 atatacaccg tttcgacgcg ctgatcgcca acggcgatcg cccatttaat ggtgccgtcg
    28261 gatgaacgct gttcttcaac cacttccggt gcgcggattt ccgccacctc tttcagtttg
    28321 ccgcgcaaca ctttgttgat gtcggtcatc tcatcaaagt tgtcgcagca atagtgatac
    28381 atccacttca tcacctgatc ggcgcggaag gttttttcac ctaaatcttt aaaaaactcc
    28441 cgcatctgct gacggttgag atccagcagg ttgatttttc catctttcgt ggtgacgttt
    28501 tcaggtgtga ctaattgttc agacatatgc tattccggcc tcgttattac acgttatggc
    28561 ccctggaggg ttgaaaaaag aaacgccccg gtgagcggct gctcgtccgg gggcgctgca
    28621 ttgtacaaat tctggcgcac ggatgccacg tttgcacgcg gcatttacga aattattaac
    28681 gggtgcgcgg gcacacttcg ccttcgccga agaaataagc gatttcgcga gcggcagatt
    28741 cgacggaatc agaaccgtgg gtaccgtttt cggtcaggct gtcagcgtaa tcagcgcgca
    28801 gagtaccagc cagtgcgttt gccggattgg tcgcgcccag cagatcgcgg tgacgctgaa
    28861 cggcgttttc accttccagc acggaaacca cgatcgggcc agaggtcatg aattcaacca
    28921 gaccatcaaa gaacggtttt ccatcgtgtt cagcataaaa gccacgtgcc tgttcaacgg
    28981 tcaggtgcag cattttggtg ccgacaattt tgaaccctgc agcttcaaag cgcgcaaaga
    29041 tattaccaat gacgtttttt gctaccgcgt tcggtttgat gatggaaaaa gtacgttcaa
    29101 tagccatttt tacctctgta aattgttctg ttgttgtctg taccagcgta cgagatggcg
    29161 cggattataa tgagcaacag ggccgttgac tattgatgaa ggtaacattt ttttaaaata
    29221 aaatgagttt tagcaacaat cagggacaaa acagactatt gcatgacaaa tttcactgtc
    29281 gcgatttgcc ccacatcatc catcaccagc aattgataat cacctttatc cgtcaaatgc
    29341 agggtcacgt tgcgcccgcg ttcagttaac ggttcgccat tcagaaacca ccagcgttca
    29401 cctgcccctc cacttgattg caacggcaaa gtcgcttccg ctgcgcccgg taaacgttta
    29461 ataatcgcgc catcgcgaac gcctgtcagc tgcagaggga gctgggcatc gtgaccgtat
    29521 ggcgggcaaa ttgtcgaggc cggtggtaag cgcacagcgc gacgttctga tgcgggcagc
    29581 cagggttcca gcggtagcgg ccagacattt accatttctt gcttcgcctg cgggcagtca
    29641 gcggcgacgc gctgcccttt gtcatccagc cagatgggga agcgaatgcc attaatccct
    29701 tcctgctctg acagtaacag cgttggcggc tggctgccat ccagcagcca ggttgccagg
    29761 cggcggcgac agttaccgtc accttccggc aaagactgtc cgcccggcca gcagataacg
    29821 ccacgagtga ctgagttcgg gcgcgggtct tccggcagat tcgcactgcg cgacagtaag
    29881 atattattga cctgattcaa caatggtacg gcactggcaa agccaaactg accaacaacg
    29941 ggcgtgccgt caggtctgcc agtccagatc ccaatgacat agcgagcgtt aaccccaatc
    30001 gcccaggcgt cacgatagcc atagctggtg cccgttttcc atgccagtgg ggcgacgcgc
    30061 ggcaaggcac tatccggcaa gggttgcgct tcatcagcca taatccggcg aatgatccac
    30121 gccgcccccg acgacattaa aggccgttca agcagcggat cgtcaggctg taagcgcaat
    30181 ttgcctgcct tgccgtggcg agcaaacgcg gtatacgctg ccgccatatc ttccagtttt
    30241 gcaccagcgc cgccgagaat gagtgaaaga ttcggcgcag cgccgttggg caaatataac
    30301 agcaatccaa cattgcgtaa ctttgccgca aaccgtttcg gtccataggc ttccagcacc
    30361 tgcacagcag gtaagttcag cgagcgcacc agcgcctcgc tcatgttgat cgggccatga
    30421 aaaccgctat caaagttacc tagtcgataa tcaccggtgc gccgggggac gtcttgcagc
    30481 agtgatgccg ggtggatcaa gccttcatcc agcgccagac cataaacaaa cggtttgagc
    30541 accgatcctg gcgatcggat cgcattgacc atatcaacat gaccaaagcg tgaatcatcg
    30601 ttgagatcaa ccgatcccac ccagccgcga acacgcatat cggtatgatt aaccacgatc
    30661 atcgccagtg agctgcgcgg tggcaaccgc cctttccagt tttgcgccag ttcttccaga
    30721 cgtcgttgaa gaccggcatc cagcgtagta acgattttgt cgcttttgct tttaccgagc
    30781 atcatgcgcg aaaacagcgg tgccagttgc ggcatttgtc ggggtgccag ccagatgggt
    30841 tcttcccttg actcttttac ctgctcacgg gaccacacac cttgcgcagc catccgttcg
    30901 agcactttat tacgcgtatt acgcgcggct tcggcacgct ccggccaacg atccgggcgc
    30961 agtcggctgg gcgcttgcgg taacaccgcc agcattgccg cctaggaata gcttaaattc
    31021 gcaggcgatt ttccgagata agcccaactt gccgcaccga tcccctgcaa cgtaccgcca
    31081 aacggagcgc ggttaagata caagatcaga atttcacgct tagacaaatg ccactccagt
    31141 tgcaacgcgc gccagagctg gagaattttg ccaccaaatg ttttggggtg aggatcaagc
    31201 agacgagcaa cctgcatagt gagcgtgctg ccaccggaaa taacccgtcc cgaagtgaga
    31261 tcttgccatg ctgcgcgcgc caccgagaat ggattaaccc ccggatgctt ccagaaccag
    31321 cgatcttcat aattgatcag cgcttcaagg taacgtgccc ctattaagtt gtcaagcatg
    31381 ttatgacccc tgcggggtat aaaaagtccc gtcgcgcatc atggcgaaca gaacgtcgca
    31441 gcgtcgtctc gccagggcga taagcacctg attgtgtcgt tttccctgac tcattttgcg
    31501 g
//
LOCUS       DADLSB010000030        31367 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.030, whole genome shotgun sequence.
ACCESSION   DADLSB010000030 DADLSB010000000
VERSION     DADLSB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.030"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(69..1013)
                     /gene="nikB"
                     /locus_tag="JN331_001376"
     CDS             complement(69..1013)
                     /gene="nikB"
                     /locus_tag="JN331_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY7259563.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(1013..2587)
                     /gene="nikA"
                     /locus_tag="JN331_001377"
     CDS             complement(1013..2587)
                     /gene="nikA"
                     /locus_tag="JN331_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY7259564.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADN
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(2698..3285)
                     /gene="acpT"
                     /locus_tag="JN331_001378"
     CDS             complement(2698..3285)
                     /gene="acpT"
                     /locus_tag="JN331_001378"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY7259565.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(3340..4389)
                     /locus_tag="JN331_001379"
     CDS             complement(3340..4389)
                     /locus_tag="JN331_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7259566.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RNLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            4488..5738
                     /locus_tag="JN331_001380"
     CDS             4488..5738
                     /locus_tag="JN331_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7259567.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPEHVPGAASSS"
     gene            complement(5742..6299)
                     /gene="dcrB"
                     /locus_tag="JN331_001381"
     CDS             complement(5742..6299)
                     /gene="dcrB"
                     /locus_tag="JN331_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY7259568.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(6372..7037)
                     /locus_tag="JN331_001382"
     CDS             complement(6372..7037)
                     /locus_tag="JN331_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY7259569.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQRFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            7258..7503
                     /gene="tusA"
                     /locus_tag="JN331_001383"
     CDS             7258..7503
                     /gene="tusA"
                     /locus_tag="JN331_001383"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY7259570.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(7605..9803)
                     /gene="zntA"
                     /locus_tag="JN331_001384"
     CDS             complement(7605..9803)
                     /gene="zntA"
                     /locus_tag="JN331_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY7259571.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTVNALRLLRRR"
     gene            complement(9877..10503)
                     /locus_tag="JN331_001385"
     CDS             complement(9877..10503)
                     /locus_tag="JN331_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY7259572.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            10644..11003
                     /locus_tag="JN331_001386"
     CDS             10644..11003
                     /locus_tag="JN331_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY7259573.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(11006..11275)
                     /locus_tag="JN331_001387"
     CDS             complement(11006..11275)
                     /locus_tag="JN331_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY7259574.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(11265..11861)
                     /gene="rsmD"
                     /locus_tag="JN331_001388"
     CDS             complement(11265..11861)
                     /gene="rsmD"
                     /locus_tag="JN331_001388"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY7259575.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            12011..13507
                     /gene="ftsY"
                     /locus_tag="JN331_001389"
     CDS             12011..13507
                     /gene="ftsY"
                     /locus_tag="JN331_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY7259576.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            13510..14178
                     /gene="ftsE"
                     /locus_tag="JN331_001390"
     CDS             13510..14178
                     /gene="ftsE"
                     /locus_tag="JN331_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY7259577.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            14171..15229
                     /gene="ftsX"
                     /locus_tag="JN331_001391"
     CDS             14171..15229
                     /gene="ftsX"
                     /locus_tag="JN331_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY7259578.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            15474..16328
                     /gene="rpoH"
                     /locus_tag="JN331_001392"
     CDS             15474..16328
                     /gene="rpoH"
                     /locus_tag="JN331_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY7259579.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            16599..17688
                     /locus_tag="JN331_001393"
                     /pseudo
     CDS             16599..17688
                     /locus_tag="JN331_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
     gene            complement(17887..18270)
                     /gene="panM"
                     /locus_tag="JN331_001394"
     CDS             complement(17887..18270)
                     /gene="panM"
                     /locus_tag="JN331_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY7259580.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTALQGGWEKR"
     gene            18694..19803
                     /gene="livk"
                     /locus_tag="JN331_001395"
     CDS             18694..19803
                     /gene="livk"
                     /locus_tag="JN331_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY7259581.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLLNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            19851..20777
                     /gene="livH"
                     /locus_tag="JN331_001396"
     CDS             19851..20777
                     /gene="livH"
                     /locus_tag="JN331_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY7259582.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            20774..22051
                     /locus_tag="JN331_001397"
     CDS             20774..22051
                     /locus_tag="JN331_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY7259583.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGRDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            22048..22815
                     /gene="livG"
                     /locus_tag="JN331_001398"
     CDS             22048..22815
                     /gene="livG"
                     /locus_tag="JN331_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY7259584.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            22817..23530
                     /gene="livF"
                     /locus_tag="JN331_001399"
     CDS             22817..23530
                     /gene="livF"
                     /locus_tag="JN331_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY7259585.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            23927..25243
                     /gene="ugpB"
                     /locus_tag="JN331_001400"
     CDS             23927..25243
                     /gene="ugpB"
                     /locus_tag="JN331_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY7259586.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNLGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            25341..26228
                     /gene="ugpA"
                     /locus_tag="JN331_001401"
     CDS             25341..26228
                     /gene="ugpA"
                     /locus_tag="JN331_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY7259587.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            26225..27070
                     /gene="ugpE"
                     /locus_tag="JN331_001402"
     CDS             26225..27070
                     /gene="ugpE"
                     /locus_tag="JN331_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY7259588.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            27150..28037
                     /gene="ugpC"
                     /locus_tag="JN331_001403"
     CDS             27150..28037
                     /gene="ugpC"
                     /locus_tag="JN331_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY7259589.1"
                     /translation="MADGEFIVMVGPPGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMNVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            28059..28802
                     /gene="ugpQ"
                     /locus_tag="JN331_001404"
     CDS             28059..28802
                     /gene="ugpQ"
                     /locus_tag="JN331_001404"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7259590.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(28789..29229)
                     /locus_tag="JN331_001405"
     CDS             complement(28789..29229)
                     /locus_tag="JN331_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY7259591.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            29349..31091
                     /gene="ggt"
                     /locus_tag="JN331_001406"
     CDS             29349..31091
                     /gene="ggt"
                     /locus_tag="JN331_001406"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY7259592.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAAPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(31150..>31367)
                     /locus_tag="JN331_001407"
     CDS             complement(31150..>31367)
                     /locus_tag="JN331_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7259593.1"
                     /translation="NRGVASALMREMIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFG
                     FEIEGTGKKYALRNGEYVDAYYMARVK"
BASE COUNT         7306 a         8101 c         8748 g         7212 t
ORIGIN      
        1 aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc gggaagagag
       61 gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag cgcggcgttg
      121 agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag cgtaaagcac
      181 tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc gacgccaggc
      241 caggcaaaga tgttttcgat aatcatcgtc ccgccaatca attcgccaat gtgcatcccc
      301 acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc ggtctgtttg
      361 tcattcaggc cgcgtagccg cgcccaggtg acgtgacgct gaccggcgac gtccagcata
      421 ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcgatgga aaccgcaggt
      481 aaaatgatgt gctgccagcc gccgtagccc atcgcgggta gccattgcag atacaccgaa
      541 aacgccatca ctagcaaaaa tgccagccag aagttgggca tcgacacgcc aagaaacgcg
      601 ataaagcgca cggcgaaatc cggcaaacga tcgcggtggc gcgccgccca gataccgagc
      661 ggtacggaag tgagcagaat taataccagc gctgcacctg caagttccag cgtggcgggc
      721 aggaagttca gcatatcgtc cagtaccggg cgttggctgg cgaatgagat accaaagtca
      781 agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg atccagcccg
      841 agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga taaacgcaga
      901 taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat caccgaggcg
      961 gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa cattaaggtt
     1021 tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag gggatattac
     1081 ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga taaaccgcct
     1141 cgtcatgcag acgggtcaga atgtcgcgat acagcgcctg acgttgcgtt tcgtcatggg
     1201 tcgccagcac ttcgccgatc tctttatcaa tcagcgggtt gtcggctaat ccttgctgtg
     1261 cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt ggatcgtatg
     1321 gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga cgagcataga
     1381 tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc atatcagcct
     1441 gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac agttcaatgc
     1501 gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc caaccggctt
     1561 tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg ccgagattag
     1621 cgtaaggcac agaaggggca aacagggtgt cggcgacctg ctgggtgcca tataacgcgt
     1681 tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact gccagctcgt
     1741 tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc tgtgacagtt
     1801 gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt aataaccctt
     1861 cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg gtagtcgggt
     1921 ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag tagttttcgt
     1981 tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac ggtccggtgc
     2041 caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag ggagcgataa
     2101 aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg cttttcaggg
     2161 taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt gccagctcca
     2221 gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc gccgcctcgg
     2281 catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag gtccaggttt
     2341 taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa ccgtctgcct
     2401 gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta ggcgtgtaaa
     2461 ggtgcgggtt tagtggcccg acattcaccg gccaggcggt ggtgatttca tctggtgcag
     2521 cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg cggagtgtgg
     2581 agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga cgattttaag
     2641 tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt gggcggatca
     2701 gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg tgggggtgca
     2761 gatcgcaagg cttaaatttt ctagctgaca atggctgact gacagcgagg agtgataggt
     2821 gctatctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga tggcttcttt
     2881 gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg cgtccatttc
     2941 agcgtgttcc ccgaggctga acacggcgtt cgccagccag cgccagttgg cgcgcgggcg
     3001 aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc aacagcagag cgatatcgtc
     3061 accgctatgg cttaagttga accatagcgg cgtttccggc gcgaatgcag gtttgccttg
     3121 ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg cacgccccgc
     3181 cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg gtggcagtgg
     3241 agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag ggccaacgtt
     3301 taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta accgactttt
     3361 cggtctgccc ggccccagta aaatcgccag tttgctgcca cctttcgtcg tttccatcca
     3421 gattttacac acgctggtta atggcaccga aagtagcatc cctaccgggc cgagcagcca
     3481 tccccagatt aacaatgaaa gaaataccac catggtggac atccccaggc gatggcccat
     3541 catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca atgcgccgac
     3601 cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg gtacggcgga
     3661 aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg cccacatcag
     3721 cgcaaactgt acgcccatta gcgccagccc cagccagacg atgacacctg tccataaact
     3781 gagtagcgtc tttaacgcca gataatgcga aacgccttta agtgcgcggt gtaatcctgc
     3841 gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt ggcgcacttc
     3901 aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg ccccggaaag
     3961 cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc gctgcagcat
     4021 tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt taaaaaggtt
     4081 gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa acgatgccgc
     4141 cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa tcgtaatggc
     4201 aacggggcgt tgtactcccc gacgaataaa ccaggtaacg agcgggttga ggacgatggc
     4261 aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa tgcccgcgag
     4321 gatcactacc agcgaggcca acttgagcag aatgtgcatg cccgttttat cgggttgagg
     4381 ggtttccatt ggggcttcct tgtgactttt tgtgttaagt gtagcgggag tcacgccagc
     4441 aatattctga ttcttactgc tgattttcac gtcagcacat ggtaaaaatg aaacactgtt
     4501 gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta aacggattgc
     4561 gcctgaattt gcgcattgtc tccattgtca tgtttaactt cgccagctac ctcaccatcg
     4621 ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt agcgccttct
     4681 gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgc cctcatgccg
     4741 gacgttacgc cgatttgctg ggacccaaaa agattgtcgt cttcggttta tgcggctgct
     4801 ttttgagcgg tctggggtat ctgacggcag gattaaccgc cagtctgcct gtcatcagcc
     4861 tgttattact ttgcctgggg cgcgtcatcc ttgggattgg gcaaagtttt gccggaacgg
     4921 gatcgaccct atggggcgtt ggagtggttg gctcgctgca tatcgggcgg gtgatttcgt
     4981 ggaacggcat tgtcacttac ggggcgatgg cgatgggtgc gccgttaggc gtcgtgtttt
     5041 atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg ctggtggcca
     5101 ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaaccg ctgccgtttc
     5161 gcgcggtgct tgggcgcgtc tggctgtacg gtatggcgct ggcactggct tccgccggat
     5221 ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg gacggtgcgg
     5281 ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta ttccctaacg
     5341 gcattaaccg tatcggtggc ttaaacgtag cgatgatttg ctttagcgtt gagataatcg
     5401 gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaaatcggc gtcttactgg
     5461 cgggggccgg gttttcgctg gtgttcccgg cattgggtgt agtggcggta aaagcggttc
     5521 cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat ttatcgcttg
     5581 gcgtgactgg accactggct gggctggtga tgagctgggc gggcgtaccg gtgatttatc
     5641 tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta aaaaaacggc
     5701 ctccggaaca cgtccctggg gccgcctcat catcttaaaa cttactgaat aaccagcgta
     5761 ttaatgatgt tttctgcggt ggtttgcgct ttttgctgat cgtcagctgg cagcgtaatt
     5821 tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta cgccgtctgg
     5881 cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag ctcaatggct
     5941 ttattggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc cagacgcttc
     6001 gccaacaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac tgctttctgc
     6061 ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt accgctctgg
     6121 tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt gacgggattc
     6181 ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc tttatcatca
     6241 caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag attgcgcatt
     6301 gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat cacaactctg
     6361 ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc cagtcttttc
     6421 aacagcatat tgagtaatac gccatacatt ggcaggaaga aaacgatact gattaacact
     6481 ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa ggcatccggg
     6541 ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc gaacagtgtg
     6601 gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa aacgtgcacg
     6661 tcgaggattt gccccagcgc gtaggccatg aaactggcgg tggcgatacg ggcgacaaac
     6721 aggttgaagt gggcgagtgc gccgaatctc tgccaggaac ccatatagaa tagcgacgag
     6781 atgacgtagg agattaataa cgcagggatc attaccgcga agataatgcg tcgggccagc
     6841 ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagaa aaataaacgg aaagctaaac
     6901 gcgccccagg tggtatggaa accaaaaatg gagacgggaa gctgaaccag atagttactg
     6961 gaggtgatca ccagaagatg aaataacgat aaccagaaca acgccttata gcgttgagtt
     7021 tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg gaacccaata
     7081 cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc gagccgccgc
     7141 atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt taacctagtt
     7201 tgcttacgga ctcacagggc gtaaactagc gccgtttttt taagtgatga gaagaaaatg
     7261 accgatctct tttccagccc tgaccacaca ctcgacgcgc ttggcctgcg ctgcccggaa
     7321 ccggtgatga tggtgcgcaa aaccgtgcgc aatatgcagc ctggcgaaac gttgctgatt
     7381 atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt tatggaacac
     7441 gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg taaaggcggt
     7501 tgataggggc tgattggctt cgatgccgcc ttttcctcac cccctaaccc tctccccaga
     7561 ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct cctgcgcaac
     7621 aaccttaacg cattcaccgt caccagcacc gtcgcacccg tatctgccag cactgccagc
     7681 cacaacccgg tcatccctaa aagcgtggtg acgaggaaga tccctttcag tcccagcgca
     7741 atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat catttgcacc
     7801 aggccgcgca ggtggttatg ggttaatgct gcgtcggcgg tttccagcgc cacgtctgtg
     7861 ccgctaccca tagcaatccc gatggcggca gctttcatcg ctggcgcgtc gttaatacca
     7921 tcaccgacca tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt gactttatct
     7981 tccggcaata ggcccgcttt aaactccagc cccagctccc cggcaattgc cgccgctgcg
     8041 cgtggattat cgccggtgag gatcactcct ttaaccccca gcgcgttcag ttcactgatg
     8101 gcagttgcag cgtcggcgcg cagggtatcc tgcaatgcaa tgatacccag cacgtcatca
     8161 ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat caaaccagca
     8221 aatgcatcag cgggatgttt cccggcagcg catatcaaca cgcgctcacc attaacctgc
     8281 gcttcaatgc cagacccgac cagtgcccgc tgtgattcgg cggtgggaat ggcgagttca
     8341 gcaacctgtg cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc ttgctcgacc
     8401 gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg aatcgcggta
     8461 acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacttg ggtaacacga
     8521 cctagttgtt ccagcgccgc accgccttta attaacgccc cacgacgcgc tgctgctgcc
     8581 agcccggagg ttatcgccgc aggcgttgag ataactaacg cacacgggca accaatcagc
     8641 agcagcgtca gacctttata aatccactcc tgccagctgg cggcaaacag cagcggcggc
     8701 actagcgtta ccagcagggc gacggccata atcgctggcg tataaatacg gctgaaacgg
     8761 tcgataaacc gctcaatggg ggcgcggcgc tcttcggctt cttcgatcag tttcagaatc
     8821 cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag acggtctacg
     8881 ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat ggattctccg
     8941 gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc ggcaggcaaa
     9001 cgcccacctg cggcgacttc aatcacatcg ccaggccgca ggctgttaat cgccacctct
     9061 tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat taacgcgcta
     9121 acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat caaaaacagc
     9181 agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat agcggctacg
     9241 ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa tgcctgacga
     9301 gcaatcgggt acagcccaac tagcgtggtc gcgataaacg ccagttgccc gaacggatga
     9361 ttgaactgct ccagacccca gctgattgcc atcatcacga taagcgtaat cagcggcaga
     9421 ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg cagggaatag
     9481 cccgcttttt gcaccgcaga ttcaacctgg gcacggatgt cattgtcggc atcaaccacc
     9541 agtttttcgg tggcgaacaa cacctgcacc tgattcacgc ctgcaagctg gcgcacggca
     9601 ttttctacct tgcgcgcaca ggcggcgcag tccatgccgc tgactttcca gctatagcgg
     9661 gtgccggaga cgttttcaga gagcgttggc gagctggagc atgcgccgtc gcagcaacag
     9721 tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg ggctttcttg
     9781 ccgtgattgt caggtgtcga catggcatcc tccggttaag ttttttctca ttaaccgaag
     9841 gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagtg agcggacgat
     9901 gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac ggaagcgtcg
     9961 gcgatagtgg ctccccagcc agacaaagtt actgataaac agcaacgaag cgccaacaaa
    10021 cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctgcca gccacaccat
    10081 caccagcgtc atgccgataa acgtgcagat aggccaacgg tactcttcca gccgcgtcca
    10141 gataatcgcc agtagcagcg cacccagaac cagcaacacc agtggcagag gccagaagaa
    10201 agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga ggaaaaacgc
    10261 gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc ccagcagtga
    10321 ggcgcacagt cccgccagca ccagatagct aatggcgtcg aacatcggtg cttgccaggc
    10381 cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc aggttggccc
    10441 acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa acgaccaaag
    10501 catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg tcttgcttcc
    10561 agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat gcttaattcc
    10621 gcattttccg atgagggata aagatgagta aaccacctct tttctttatt gttatcattg
    10681 gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa aaagctgata
    10741 atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg gaaaaaccga
    10801 ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt atgcgctatg
    10861 aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc gacgcccagc
    10921 agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga acgcgctttg
    10981 ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc tgccagacca
    11041 gcagttcaaa cacgccgaaa aggaaaatcc gcaccttttc ggcggtggtc atctgcgggc
    11101 catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc agcacggtaa
    11161 aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg gaagggatgc accagattga
    11221 ggattaaaaa tccccaaacg cagagcatta acaaacgacc aatattaatc agcatcactt
    11281 tctccttgtg cttcgtgttg atacagccga taagccacct gacccgccac tttttcccga
    11341 tgtaatgacc agtttgccgg aacagtgggc agaccgtttt cgacttcgct ttcgacataa
    11401 atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt ctcttctaac
    11461 aagccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc tttttgcgcc
    11521 aggaatgaca ttgcgttgct gttcaccacg cgtgcattgc ctgcttttag tgtcgccaga
    11581 ttcttaatta actgctgaga aaccacgcga tccatctcaa tcaacgttgc ccccgcagcg
    11641 tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc cagacattgg
    11701 gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg gtcggtggtg
    11761 gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg cccgccaata
    11821 atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg ctcaatccgc
    11881 cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga ctatttcatc
    11941 atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac agcgaggagt
    12001 gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg gctttggtca
    12061 aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg ttgtagaaga
    12121 aatcgttcag gcgcaagagc ctgtgaaggc ttctgaacaa gccgttgaag agcagccgca
    12181 ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag tcactgaaca
    12241 ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt gttgcacagc cggaaccggt
    12301 cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt tgccggaaga
    12361 cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa ccgtagagat
    12421 tgtcgaagcg gcagaagaag aagcggctaa agaagaaatc accgacgaag agctggaagc
    12481 acaggcgctg gctgccgaag cggcagaaga ggcggtgatg gtagttcctc cggcagaaga
    12541 agagcagccg gttgaagaaa ttgcccagga gcaagaaaaa ccgaccaaag aaggcttctt
    12601 cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg gatttatcag
    12661 cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgaa gagctggaag aacagctgtt
    12721 gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga cggaaggcgc
    12781 atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag aagagatggg
    12841 cgagattctg gcgaaagtcg atgagccgct gaatgttgaa gataaaacgc cgttcgtgat
    12901 cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc tggcgcgtca
    12961 gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc gtgcagctgc
    13021 ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg cccagcatac
    13081 cggtgcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag cgcgtaatat
    13141 cgacgtcctg attgccgata ccgccggacg tctgcagaac aaatcgcatc tgatggaaga
    13201 gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc atgaagttat
    13261 gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac tgttccacga
    13321 agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga aaggcggggt
    13381 aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg tcggcgaacg
    13441 tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt ttgcccgaga
    13501 ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg gagacaggcg
    13561 ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac cggtcattcc
    13621 ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc cagcgccggg
    13681 aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt tccgtttctg
    13741 cgtcgccaga ttggcatgat tttccaggat caccatctac tgatggaccg tactgtctac
    13801 gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat tcgtcgccgg
    13861 gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt ccctattcag
    13921 ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa taagcccgcg
    13981 gtactgctgg cggacgaacc gaccggtaac ctggacgacg cgttgtcgga agggattcta
    14041 cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac gcacgacatt
    14101 aacctgattt cacggcgttc ctatcgcatg ctcaccctga gcgatggtca cttgcatgga
    14161 ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg gtgggcgtct
    14221 ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg caccgaaacg
    14281 cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg aacaggtgcg
    14341 ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca cgtttttaac
    14401 ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg tgtacaaaaa
    14461 cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt acttgcaaaa
    14521 aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg agcaaggcgt
    14581 ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta actggtctgg
    14641 ttttggtggt gcgctggata tgctggaaga aaacccgctt ccggcagtgg cggtggtgat
    14701 cccgaaactc gattttcagg ggacggaatc actcaatacg ctgcgtgatc gtatcacgca
    14761 gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc tggcggcgtt
    14821 gaccgggctg gttgggcgcg tttcggcgat gatcggcgtg ttgatggtgg cggcagtgtt
    14881 cctcgtcatc ggcaacagcg tacgtctgag catctttgct cgccgtgact ccatcaacgt
    14941 gcagaaactg attggtgcga cggatggttt catccttcgc ccgttcctgt acggcggtgc
    15001 gctgctggga ttttctggcg cattgttgtc attaattttg tcagaaattc tggtactgcg
    15061 tttgtcgtca gcggtggcgg aagtggcaca ggttttcgga acgaagtttg atatcaatgg
    15121 tttatcgttc gatgaatgcc tgctattgct gctggtatgc tcgatgattg gctgggtggc
    15181 agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat aaaagcgtgt
    15241 tatactcttt ccctgcaatg ggttccgtag cagggaaaga gaccccgttg tctcttcccg
    15301 gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc attgaacttg
    15361 tggataaaat cacggtctga taaaacagtg aatgataacc tcgttgctct taagctctgg
    15421 cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt tgaatgactg
    15481 acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac atccgggcag
    15541 ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa aagctgcatt
    15601 accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg tttgttgttc
    15661 atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt caggaaggta
    15721 acatcggcct gatgaaagca gtgcgccgtt ttaacccgga agtgggtgtg cgcctggtct
    15781 ccttcgccgt ccactggatc aaagcagaga tccacgaata cgttctgcgg aactggcgta
    15841 tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg cgtaaaacca
    15901 agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt gaactgggcg
    15961 taaccagcaa agacgtacgt gagatggaat cacgtatggc ggcacaggac atgacctttg
    16021 acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc tatctgcagg
    16081 ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag caggcggcaa
    16141 accgtctgac cgacgcgatg catggtctgg acgaacgcag ccaggacatc atccgtgcgc
    16201 gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt tacggtgttt
    16261 ccgctgaacg tgtgcgccaa ctggaaaaga acgcgatgaa aaaacttcgc gccgctattg
    16321 aagcgtaatt tccgctatta agcagagaac cctggatgag agtccggggt ttttgttttt
    16381 tgggcctcta caataatcaa ttccccctcc ggcaaaacgc caatccccac gcagattgtt
    16441 aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt tagcagagta
    16501 tgctgctaaa gcacgggtag tcatgcataa aacgaaataa agtgctgaac aacaacatca
    16561 caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag ggtaaagcgt
    16621 tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa gatattaaag
    16681 tcgcggtcgt gggtgcaatg tccggtccgg ttgcgcagta cggtgaccag gagtttaccg
    16741 gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc aacaaactgc
    16801 aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg gcgaacaaag
    16861 tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca acgcagcctg
    16921 cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca accgcgccgg
    16981 agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac tccgaccagg
    17041 ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt gctatcgttc
    17101 acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc ctgaagaaag
    17161 gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat ttctcaacgc
    17221 tggtggcgcg tctgaaaaaa gagaatatcg acttcgttta ctacggcggt tatcacccgg
    17281 aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactgga aggtgtggct
    17341 aacgtttcgc tgtctaacat tgcgggcgaa tcagcggaag ggctgctggt gaccaagccg
    17401 aagaactacg atcaggttcc ggcgaacaaa cccattgttg atgcgatcaa agcgaaaaaa
    17461 caggacccaa gcggcgcatt cgtctggacc acctacgccg cgctgcaatc tttgcaggcg
    17521 ggcctgaatc agtctgacga tccggctgaa atcgccaaat acctaaaagc gaactccgtg
    17581 gataccgtga tggggccact gacctgggat gagaaaggcg atctaaaggg ctttgagttc
    17641 ggcgtatttg actggcacgc caacggcacg gcgaccgatg cgaagtaatt gttaatcggc
    17701 aactttgggt tgccgccaaa ttgctaatat cgaatacgtt gcttcatgcc ggatgcggcg
    17761 taaacgcctt atccggccta caagatccaa agaaatcagt aaattgcagc acacattata
    17821 ggcctgataa gcgtagcgca tcaggcaatt tctcgttggt catcactttt gaaatcaact
    17881 tgacgattaa cgcttctccc agccgccctg cagtgccgta aatcccagtg cctgcataaa
    17941 cgccgtcatc acaccgcgat cttccacgcc tgcatccgcc atccaccagc atgaaacgcc
    18001 aggattgtta cgcaaaacct cttccagcag atattgcccc acaccgcgac ggcgggtgac
    18061 ttcccgcacg cgtagagaat ccagtgctcc ctcggtgccg cttaaggtta cccgcacggc
    18121 cgcaagtaga cgctcgttaa aacgcgcggc gtagatacgg tggttatcgt caacctgtaa
    18181 agaggaaggg gaatactccg gccagatctt ttgcaggtca atccggtcct ggtcgctaaa
    18241 gttttctaat cgaatgatgg tcagcttcat gggtaacccg tgtaaatcac aaaagtggaa
    18301 ccagtgtagc gaaataattt aatcggaggc tttctctttt ttatttcttt tggcaggtga
    18361 ttaatttttt aacagcaata attacaaagt taaaacatta gagaatgaaa aatgtccagc
    18421 ataatcccct gaatgatagt gaattattcc gccctttgtg ccgttatttt atgctgacaa
    18481 aggcactttt ttctgtttat ctatcaataa attcagaata ttatctgttc ttaatcgact
    18541 gaaaaatagg gattttaatc gctatcatca caaaatactg cgctaacccc ttaatcagac
    18601 aggcaaaaac agtgcagtat aaaaaaagaa cagtctgatt tgttaacaca taaaaacaaa
    18661 gcaacacaac atcacgaatg gggatttttg actatgaaac ggaatgcgaa aactatcatc
    18721 gcagggatga ttgcactggc aatttcacac accgctatgg ctgacgatat taaagtcgcc
    18781 gttgtcggcg cgatgtccgg cccgattgcc cagtggggcg atatggaatt taacggcgcg
    18841 cgtcaggcga ttaaagacat taatgccaaa gggggaatta agggcgacaa gctggttggc
    18901 gtggaatatg acgacgcctg cgacccgaaa caagccgttg cggtcgccaa caaaatcgtt
    18961 aacgacggca ttaaatacgt tattggtcat ctgtgttctt cctctaccca gccagcgtcg
    19021 gatatctacg aagacgaagg tattctgatg atctcgccgg gagcgaccaa cccggagctg
    19081 acccaacgcg gttatcaaca cattatgcgt actgccgggc tggactcttc ccaggggcct
    19141 acggcggcaa aatacattct tgagacggtg aagccccagc gcatcgccat cattcacgac
    19201 aaacaacagt atggcgaagg gctggcgcgt tcggtgcagg acgggctgaa agcggctaac
    19261 gccaacgtcg tcttcttcga cggtattacc gccggggaga aagatttctc cgcgctgatc
    19321 gcccgcctga aaaaagaaaa tatcgacttc gtttactacg gcggttacta cccggaaatg
    19381 gggcagatgc tgcgccaggc ccgttccgtt ggcctgaaaa cccagtttat ggggccggaa
    19441 ggtgtcggta acgcatcgct gttgaacatt gccggtgatg ccgccgaagg catgttggtc
    19501 actatgccaa aacgctatga ccaggatccg gcaaaccagg gcatcgttga tgcgctgaaa
    19561 gcagacaaga aagatccgtc cgggccttat gtctggatca cttacgcggc agtgcaatct
    19621 ctggcaactg cccttgagcg taccggcagc gatgagccgc tggcgctggt gaaagattta
    19681 aaagctaacg gtgcaaacac cgtgattggg ccgctgaact gggatgaaaa aggcgatctt
    19741 aagggatttg attttggtgt cttccagtgg cacgccgacg gttcatccac ggcagccaag
    19801 tgatcatccc accgcccgta aaatgcgggc gggtttagaa aggttacctt atgtctgagc
    19861 agtttttgta tttcttgcag cagatgttta acggcgtcac gctgggcagt acctacgcgc
    19921 tgatagccat cggctacacc atggtttacg gcattatcgg catgatcaac ttcgcccacg
    19981 gcgaggttta tatgattggc agctacgtct catttatgat catcgccgcg ctgatgatga
    20041 tgggcattga taccggctgg ctgctggtgg cggcgggatt cgtcggcgca atcgtcatcg
    20101 ccagcgccta cggctggagt atcgaacggg tagcttaccg cccggtgcgt aactctaagc
    20161 gcctgattgc gcttatttcc gcaatcggta tgtccatctt cctgcaaaac tacgtcagcc
    20221 tgaccgaagg ttcgcgcgac gtggcgctgc cgagcctgtt taacggtcag tgggtggtgg
    20281 gccatagcga aaacttctct gcctctatta ccaccatgca ggcggtgatc tggattgtca
    20341 ccttcctcgc catgctggcg ctgacgattt tcattcgcta ttcccgtatg gggcgcgcct
    20401 gtcgtgcctg cgcggaagat ctgaaaatgg cgagcctgct tggcatcaac accgaccggg
    20461 tgattgcttt gacctttgtt attggcgcgg cgatggcggc agtggctggc gtactgctcg
    20521 gtcagttcta ctgcgtcatt aacccctata tcggctttat ggccgggatg aaagccttta
    20581 ccgctgcggt gctcggtggg attggcagca ttccaggggc gatgattggc gggctgatcc
    20641 tggggattgc ggaggcgctc tcttctgcct atctgagtac ggaatataaa gatgtggtct
    20701 cattcgccct gctgattctg gtgctgctgg tgatgccgac cggtattctg ggtcgcccgg
    20761 aggtagagaa agtatgaaac cgatgcatat tgcaatggca ctgctctctg ccgcgatgtt
    20821 ctttgtcctg gcgggcgtct ttatgggcgt acaactggag ctggatggca ccaaactggt
    20881 ggtcgacacg gcttcagaca tccgttggca gtggatgttt atcggcacgg cggtggtctt
    20941 tttcttccag cttttgcgac cggctttcca gaaagggttg aaaagcgttt ccgggccgaa
    21001 gtttattctg cccgcgattg atgggtccac ggtgaagcaa aagctgttcc tcgtggcgct
    21061 gctggtgctt gtggtggcgt ggccgtttat ggtgtcacgt gggacggtgg atatcgccac
    21121 tctgaccatg atctacatta tcctcggtct cgggctgaat gtggttgttg gtctttctgg
    21181 tctgctggtg ctggggtacg gcggttttta cgccatcggc gcttacactt ttgcgctgct
    21241 caatcactat tacggtctgg ggttctggac ctgtttgccg attgccggac tgatggcagc
    21301 ggcggcgggt ttcctgctcg gttttccggt gctgcgtctg cgcggtgact atctggcgat
    21361 cgttaccctt ggtttcggcg aaattgtgcg catattgctg ctcaataaca ctgaaatcac
    21421 cggcggcccg aacggaatca gtcagatccc gaaaccgaca ctcttcggac tcgagttcag
    21481 ccgtaccgct cgtgaaggcg gcagggacac gttcagtaat ttctttggcc tgaaatacga
    21541 tccgtccgat cgcgttatct tcctctacct ggtggcgttg ctgctggtgg tgttaagcct
    21601 gtttgtcatt aaccgcctgc tgcggatgcc gctgggccgc gcgtgggaag cgttgcgtga
    21661 agacgagatc gcctgtcgtt cgctgggctt aagcccgcgc cgtatcaagc tgaccgcttt
    21721 taccatcagc gccgcatttg ccggttttgc cggaacgctg tttgcggcgc gtcagggctt
    21781 tgtcagcccg gaatccttca cctttgctga atcggccttt gtgctggcga tagtggtgct
    21841 cggcggtatg ggctcgcaat ttgcagtgat tctggcggct gttctgctgg tggtgtcgcg
    21901 cgaattgatg cgtgatttca acgaatacag catgttaatg ctcggtggtt tgatggtgct
    21961 gatgatgatc tggcgtccgc aggggttgct gcccatgacg cgcccgcaac tgaagctgaa
    22021 aaacggcgca gcgaaaggag agcaggcatg agtcagccat tattatctgt taacggtctg
    22081 atgatgcgct tcggcggcct gctggcggtg aacaacgtca atcttgaact gtacccgcag
    22141 gaaatcgtct cgttaatcgg ccctaacggt gccggaaaaa ccacggtttt taactgcctg
    22201 accggtttct acaaacccac cggcggcacc attttactgc gcgatcagca tcttgaaggt
    22261 ttaccggggc agcaaattgc ccgcatgggc gtggtgcgca ccttccagca tgtgcgtctg
    22321 ttccgtgaaa tgacggtaat tgaaaacctg ctggtggcgc agcatcagca actgaaaacc
    22381 gggctgttct ctggcctgtt gaaaacgcct tctttccgtc gcgcccagag cgaagcgctc
    22441 gaccgcgccg ccacctggct tgagcgcatt ggcttgctgg aacacgccaa ccgtcaggcg
    22501 agtaacctgg cctatggtga ccaacgccgt ctggagattg cccgctgcat ggtgacgcag
    22561 ccggagattt taatgctcga cgaaccggcg gcaggtctta acccaaaaga aaccaaagag
    22621 ctggatgagc tgattgccga actgcgcaat catcacaaca ccactatctt gttgattgaa
    22681 cacgatatga agctggtgat ggggatttca gaccgaattt atgtggtcaa tcaggggacg
    22741 ccactggcaa acggtacgcc ggagcaaatc cgtaataacc cggacgtgat ccgtgcctat
    22801 ttaggtgagg cataagatgg aaaaagtcat gttgtccttt gacaaagtca gcgcccacta
    22861 cggcaaaatc caggcgctgc atgaggtgag cctgcatatc aatcagggcg agattgtcac
    22921 tctcattggc gcgaacgggg cggggaaaac caccttgctc ggcacgttat gcggcgatcc
    22981 gcgcgccacc agcgggcgaa ttgtgtttga tgataaagac attaccgact ggcagacggc
    23041 gaaaatcatg cgcgaagcgg tagcgattgt cccggaaggg cgtcgcgtct tctcgcggat
    23101 gacggtggaa gagaacctgg cgatgggcgg cttctttgcc gaacgtgacc agtttcagga
    23161 gcgcataaag tgggtgtatg aactgtttcc acgtctgcat gagcgccgtg ttcagcgggc
    23221 gggcaccatg tccggcggtg aacagcagat gctggcgatt ggtcgcgcgc tgatgagcaa
    23281 tccgcgtttg ttgttacttg atgagccatc gctcggtctt gcgccgatta tcatccagca
    23341 aattttcgac accatcgagc agctgcgcga gcaggggatg actatcttcc tcgtcgagca
    23401 gaacgccaac caggcgctaa agctggcgga tcgcggctac gtgctggaaa acggccatgt
    23461 ggtgctctcc gataccggcg acgcgctgct ggcgaacgaa gcagtgagaa gtgcgtattt
    23521 aggcgggtaa taacacgttg attgataagg agtcaaaaga ctcctttgag acaggtgaca
    23581 aatgtaaaat tgcctgatgc gctgcgctta tcaggcctac tgggtgagtt gcaatatatt
    23641 gaatttgcac gatcttgtag gccggataag gcgtttacgc cgcatccggc atgaaacgat
    23701 gagtaatctg tagagtttga ttcagacctt ctatttttcc gcttatccgt gccccatctc
    23761 ccattttccc tcacccacgc cgtcaccgcc ttgtcatctt tctgacacct tactatctta
    23821 caaatgtaac aaaaaagtta tttttctgta attcgagcat gtcatgttac cccgcgagca
    23881 taaaacgcgt gaattcgcgc attcggtaca acaagagaga taaacgatga aaccgttaca
    23941 ttatacagct tcagcactgg cgctcggact ggcgttaatg gggaatgcac aggcagtgac
    24001 gaccattccg ttctggcatt ctatggaagg ggaactgggt aaagaggtgg attctctggc
    24061 ccaacgtttt aacgccgaaa atccggatta caaaattgta ccgacctata aaggcaacta
    24121 cgaacagaat ttaagcgcgg ggattgccgc atttcgtacc ggcaacgctc cggctatttt
    24181 gcaggtttat gaagttggca ccgccaccat gatggcgtcg aaagccatta aaccggtgta
    24241 tgacgtgttt aaagaggcag ggattcagtt cgatgagtcg cagtttgtgc cgacggtttc
    24301 cggttactac tccgacagca aaaccggcca cttactctcc cagccgttca acagctcgac
    24361 tcccgttctc tattacaaca aagacgcctt caagaaagcg gggttagacc cggaacagcc
    24421 gccgaaaacc tggcaggatc tggcggacta ttccgcaaaa ctgaaagcct ccggtattaa
    24481 gtgcggctac gccagcggct ggcagggctg gatccaactg gaaaacttta gcgcctggaa
    24541 cggtctgccg tttgccagca aaaacaacgg ctttgacggc acggacgcgg tgctggagtt
    24601 caacaagccg gagcaggtga aacacatcgc catgctcgag gagatgaaca agaagggcga
    24661 cttcagctac gtcggtcgta aggatgaatc caccgagaag ttctataacg gtgattgcgc
    24721 gatgaccacc gcctcttccg gttctcttgc caacattcgc gagtacgcca aatttaacta
    24781 cggcgtaggt atgatgcctt acgacgccga tgcgaaagat gcgccacaaa acgccattat
    24841 cggcggagcc agcctgtggg tgatgcaggg caaagataaa gaaacgtata ccggcgtggc
    24901 gaagtttctc gacttcctcg cgaagccaga aaacgctgct gagtggcatc agaaaaccgg
    24961 ttatctgcca attaccaaag cggcgtatga cctgacccgt gagcagggct tttacgagaa
    25021 aaacctaggt gcggataccg cgacgcgtca gatgctgaac aagccgccgt tgccgttcac
    25081 caaagggctt cgtctgggca acatgccgca gatccgcgtg attgtggatg aagagcttga
    25141 gagcgtgtgg accggtaaga agacgccaca gcaggcgctg gataccgcag ttgagcgtgg
    25201 gaaccagtta ctgcgccgct ttgagaaatc gacgaagtct taatcagtgt aatgccggat
    25261 gcgtttcgct tatctgacct ggcatcgcgt gtaggccgga taagcgaagc gcatccggca
    25321 cagttcagga attaaccgta atgtcatcat cccgtccggt gttccgctcg cgctggctgc
    25381 cttatctgct ggtcgcgccg cagctcatca tcaccgttat cttttttatc tggcctgcgg
    25441 gcgaagcgtt gtggtactcg ctacaaagcg tcgatccgtt tggtttctcc agccagtttg
    25501 tcggtctgga taacttcgtc acgctgtttc atgacagcta ttatctcgac tccttctgga
    25561 cgacgataaa attcagcacc tttgtcaccg tcagcggttt gctggtatcg ctgttctttg
    25621 cggcgctggt ggagtacatc gtgcgcggca gtcgtttcta ccaaacctta atgttgctgc
    25681 cgtatgccgt ggctcccgcc gttgccgccg tattgtggat cttcctgttt aaccccggtc
    25741 gcgggctgat cacccatttt ctcgctgagt tcggctacga ctggaaccac gcgcaaaata
    25801 gcggtcaggc gatgtttctg gtggtgtttg cctcggtgtg gaagcaaatc agctacaact
    25861 ttctgttctt ctatgccgcg ctgcaatcca ttccccgttc gttgatcgaa gccgcagcca
    25921 tcgacggtgc agggccgatt cgccgcttct ttaagattgc gctgccgctt atcgccccgg
    25981 tgagtttctt cctgctggta gtgaacctgg tgtatgcctt cttcgacacc ttcccggtga
    26041 tcgacgccgc cacgtccggc gggccggttc aggccaccac gacgctgatt tataagatct
    26101 accgcgaagg ttttaccgga ctggatctgg cttcgtctgc cgcacagtcg gtggtgttga
    26161 tgttcctcgt catcgtgctg acggtggtgc agttccgcta tgttgaaagc aaggtgcgtt
    26221 accaatgatt gagaaccgtc cgtggctgac gatattcagc cataccatgc tgatcctcgg
    26281 gatcgcggtg atcctcttcc cgctgtacgt ggcgtttgtc gcggcgacgc tggataaaca
    26341 ggccgtctac gccgcgccga tgacgctcat ccccggcacg catctgctgg aaaacatcca
    26401 caacatctgg gtgaacgggg taggcacaaa tagcgcgccg ttctggcgga tgttgcttaa
    26461 cagctttgtg atggcgttca gcattacgct cggcaaaatt actgtctcga tgctctcggc
    26521 atttgccatt gtctggtttc gttttccgct acgtaacctc ttcttctgga tgatttttat
    26581 caccctgatg ctgccggttg aagtacgtat cttcccgacg gtggaagtca tcgccaacct
    26641 gaagatgctc gacagctacg ccggtttaac gctgccgctg atggcctcgg cgaccgccac
    26701 gtttctgttt cgccagttct ttatgacgct gccggatgaa ctggtggaag cggcgcggat
    26761 cgacggtgcg tcaccaatgc gcttcttttg cgacatcgtt tttccgctct ccaaaactaa
    26821 tctggcggcg ctgtttgtga tcacctttat ttacggctgg aaccagtatt tgtggccgtt
    26881 gttgattatt accgacgtgg atctcggcac caccgtggca gggatcaaag ggatgatcgc
    26941 cactggcgaa ggcaccacgg aatggaactc agtgatggcg gcgatgttgt taaccctgat
    27001 cccaccagta gtgattgttt tagtgatgca gcgtgccttc gtgcgcggcc tggtcgatag
    27061 tgagaaataa gatggcagga ctgaaattac aggcagtaac caaaagctgg gatggcaaaa
    27121 cctaggccat taaaccgctg acccttgatg tggcggatgg cgaatttatc gtgatggtcg
    27181 ggccgcctgg ctgcgggaaa tcgacgctgc tgcgtatggt tgccgggctg gagcgggtga
    27241 ccgaaggcga tatctggatc aacgaccagc gcgtgaccga aatggagccg aaagatcgcg
    27301 ggattgcgat ggtgttccag aattacgcgc tttatccgca tatgaacgtc gaagaaaaca
    27361 tggcgtgggg gctgaaaatt cgcggcatgg gcaagcagca aatagccgag cgcgttaaag
    27421 aagcggcgcg cattctggag ctggacggtc tgctcaaacg tcgcccgcgc gagctttccg
    27481 gcggtcagcg ccagcgtgtg gcgatgggac gcgcgattgt gcgcgatccg gcggtgttct
    27541 tgtttgatga gccactctct aacctcgatg ccaagctgcg cgtacagatg cgtcttgaac
    27601 tgcaacagct gcaccgtcgc ctgaaaacga cttcactcta cgttactcac gatcaggttg
    27661 aggcgatgac gctcgcccag cgagtaatgg tgatgaacgg cggcgttgcc gaacagattg
    27721 gcacgccggt ggaagtctac gaaaagcccg ccagcctgtt tgtggcgagt tttatcggca
    27781 gcccggcgat gaacctgctg acaggccgca tgaataatga aggcacgcat ttcgaactgg
    27841 acggcggtat cgtgctaccg ctaaacggtg gctaccgtca gtatgccggg cgtaaaatga
    27901 ccctcggcat tcgcccggaa catattgcgc taagctcgca agcagaagct ggtggtgcga
    27961 ctggcgcatc aggagcgccc gacggcaggc agcacgctgt ggctgcatct ggcggaaaat
    28021 cagctgcatc tttttgatgg tgaaacagga caacgagtat gagtaactgg ccttatcccc
    28081 gcatcgtcgc tcatcgtggc ggcggtaagc tggccccgga aaacaccctg gcggcaatcg
    28141 acgtcggggc aaaatacggt cataagatga tcgaatttga cgcgaagtta tcgaaagatg
    28201 gcgagatctt cctgctccat gacgacaatc tcgaacgcac cagcaacggc tggggcgtcg
    28261 cgggtgaact gaactggcag gatttactgc gcgtggatgc gggcagttgg tacagcaaag
    28321 cgtttaaagg tgagccgctg ccgttgcttt cgcaggtggc ggaacgctgt cgcgaacacg
    28381 ggatgatggc gaatatcgaa atcaaaccca ccactggcac tggaccatta acgggcaaaa
    28441 tggtggcgct ggcggcacgc caactgtggg ccggtatgac gccgccgctg ctgtcatcgt
    28501 ttgagattga tgctttagaa gctgcacaac aggcggcacc ggaactgccg cgtggtttat
    28561 tgcttgatga gtggcgcgac gactggcgcg aactgaccgc gcggctgggc tgcgtctcta
    28621 ttcatctcaa tcataagtta ctcgataaag cgcgagtgat gcagttgaaa gacgccggat
    28681 tacggattct ggtttatacc gtcaataaac cccagcgcgc ggcagagttg ctgcgctggg
    28741 gcgtggattg catctgtacc gatgcgattg acgtgattgg tccgaacttt acggcccaat
    28801 agttttcaac ggaatgtcag gctgcggcgt gctgggctga ttcagcatgt cgccgtttct
    28861 cctctccggc agcatatgct gctgattcaa cgaactatcc ggattacggt tgctgtttaa
    28921 catcccgccg ttggtgttgg gcagcatttg ctgccgggcg ggatttcgct cccccggctg
    28981 tgactgcaac acccgctgag aattattgtt tatctggttt tctaaatgct gctgttgcaa
    29041 ctgagtttgc gttttcagtt gctgattcag catccctttt tgctggattt gctgagtctg
    29101 catctgggtt tgcatccgct gctggctggg tatctgatac cccggctggt tagggttgtt
    29161 cagagtatta atgggctgtg caaagccgac aaacggcagg agtgccgtaa gaatcagaag
    29221 tcgtttcatc gcgtatcctc ctctgaagat atcctttaag tttactcgct tcccgacaaa
    29281 acgatgatta attcagagtt atataccagg cttagctggg gttgcccctt aatctctgga
    29341 gaataacgat gataaaaccg acgtttttac gccgggtggc cattgctgct ctgctctcag
    29401 gaagttgttt tagcgccgcc gccgcgcctc ctgcggcgcc cgtctcgtat ggtgtggagg
    29461 aagatgtctt ccacccggta cgcgcgaaac agggaatggt agcgtctgtg gacgccactg
    29521 ccactcaggt gggggtggat atcctcaagg agggcgggaa tgccgttgat gccgccgtgg
    29581 cggtgggcta cgcgctggcg gtaacgcatc cgcaggcagg gaatctgggc ggtggtggtt
    29641 ttatgttaat ccgctcgaaa aatggcaata ccacggctat cgatttccgc gaaatggcac
    29701 ccgccaaagc gacccgcgat atgttcctcg atgatcaggg caacccggac agcaaaaaat
    29761 cactcacttc gcatctggct tccggcacac cgggtacggt agcaggtttc tcgctggcgc
    29821 tggataaata cggcaccatg ccgctgaaca aagtcgtgca gcccgcgttt aaactggcac
    29881 gcgatggttt tatcgttaac gacgcgctgg ctgacgatct caaaacctac ggtagcgaag
    29941 tgttgccgaa tcacgaaaac agtaaagcta tcttctggaa agagggcgag ccgctgaaaa
    30001 agggcgacac gctggtgcag gcgaacctgg caaagagcct ggagatgatt gctgaaaacg
    30061 gcccggacga attctataaa ggcacgattg cggaacagat cgcccaggag atgcagaaaa
    30121 acggtggctt gatcactaaa gaagatttag cggcctataa agcggtcgaa cgcactccga
    30181 taagcggcga ttatcgcggg tatcaggttt actccatgcc accgccatcc tccggcggga
    30241 tccatatcgt acaaatcctc aatattctgg aaaacttcga tatgaagaaa tacggctttg
    30301 gcagcgccga tgcgatgcaa atcatggcag aagcggagaa atacgcctac gccgaccgct
    30361 cggaatatct tggcgacccg gattttgtca aagtcccgtg gcaggcactg accaataaag
    30421 cctatgccaa atccattgcc gatcaaattg atatcaacaa agcgaagccg tccagtgaga
    30481 ttcgccctgg caagcttgcg ccttatgaga gtaatcaaac tacccattac tctgtagtgg
    30541 ataaagacgg taacgcggtg gcggtgacct atacgctgaa caccaccttc ggtacgggta
    30601 ttgtcgcggg cgagagcggt attctgctta ataaccagat ggatgatttc tccgccaaac
    30661 cgggcgtacc gaacgtttac gggctggtgg gcggtgatgc caacgccgtc gggccgaaca
    30721 aacgcccgct gtcgtcgatg tcgccgacca ttgtggtgaa agacggtaaa acctggctgg
    30781 ttaccggtag cccaggcggt agccggatta tcaccacggt gctgcaaatg gtggtgaata
    30841 gcatcgatta tggcatgaac gtcgccgaag cgaccaatgc gccgcgtttc caccatcagt
    30901 ggttgccgga cgagctgcgt gtcgaaaaag ggtttagccc ggatacgctc aagctgctgg
    30961 aagcaaaagg tcagaaagtg gcgctgaaag aggcgatggg cagcacacaa agcattatgg
    31021 ttgggccgga cggtgagttg tacggcgcat ccgacccgcg ctcggtggat gatttaacgg
    31081 cggggtatta atatcttaag ccctcccggt aggtgagggc ttaaatgaca aacttagatt
    31141 gcaaattctc tatttcaccc gcgccatata atacgcatcg acatattcac cattacgcaa
    31201 tgcgtacttc ttaccggtcc cttcaatctc aaagccgaat tttttataga ccttaattgc
    31261 tggcgcgtta tcgacaaaca cggttagttc aatgcgatct acccgtaacc agttgtcgca
    31321 catttcaatc atctctcgca tcagggcgct ggcgacgccg cggttct
//
LOCUS       DADLSB010000031        29649 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.031, whole genome shotgun sequence.
ACCESSION   DADLSB010000031 DADLSB010000000
VERSION     DADLSB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.031"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(93..1064)
                     /gene="secF"
                     /locus_tag="JN331_001408"
     CDS             complement(93..1064)
                     /gene="secF"
                     /locus_tag="JN331_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY7259594.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFCKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(1075..2922)
                     /gene="secD"
                     /locus_tag="JN331_001409"
     CDS             complement(1075..2922)
                     /gene="secD"
                     /locus_tag="JN331_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY7259595.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(2950..3282)
                     /gene="yajC"
                     /locus_tag="JN331_001410"
     CDS             complement(2950..3282)
                     /gene="yajC"
                     /locus_tag="JN331_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY7259596.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(3305..4432)
                     /gene="tgt"
                     /locus_tag="JN331_001411"
     CDS             complement(3305..4432)
                     /gene="tgt"
                     /locus_tag="JN331_001411"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY7259597.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(4487..5557)
                     /gene="queA"
                     /locus_tag="JN331_001412"
     CDS             complement(4487..5557)
                     /gene="queA"
                     /locus_tag="JN331_001412"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY7259598.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIGRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            5650..6231
                     /gene="acpH"
                     /locus_tag="JN331_001413"
     CDS             5650..6231
                     /gene="acpH"
                     /locus_tag="JN331_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HAY7259599.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(6236..8053)
                     /gene="malZ"
                     /locus_tag="JN331_001414"
     CDS             complement(6236..8053)
                     /gene="malZ"
                     /locus_tag="JN331_001414"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY7259600.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDRILALPAISATVWMN"
     gene            complement(8209..9582)
                     /gene="proY"
                     /locus_tag="JN331_001415"
     CDS             complement(8209..9582)
                     /gene="proY"
                     /locus_tag="JN331_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY7259601.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGVGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(9658..10977)
                     /gene="brnQ"
                     /locus_tag="JN331_001416"
     CDS             complement(9658..10977)
                     /gene="brnQ"
                     /locus_tag="JN331_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY7259602.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(11384..12679)
                     /gene="phoR"
                     /locus_tag="JN331_001417"
     CDS             complement(11384..12679)
                     /gene="phoR"
                     /locus_tag="JN331_001417"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY7259603.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(12737..13426)
                     /gene="phoB"
                     /locus_tag="JN331_001418"
     CDS             complement(12737..13426)
                     /gene="phoB"
                     /locus_tag="JN331_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY7259604.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            13616..14818
                     /gene="sbcD"
                     /locus_tag="JN331_001419"
     CDS             13616..14818
                     /gene="sbcD"
                     /locus_tag="JN331_001419"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY7259605.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            14815..17958
                     /gene="sbcC"
                     /locus_tag="JN331_001420"
     CDS             14815..17958
                     /gene="sbcC"
                     /locus_tag="JN331_001420"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY7259606.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            18084..19268
                     /gene="araJ"
                     /locus_tag="JN331_001421"
     CDS             18084..19268
                     /gene="araJ"
                     /locus_tag="JN331_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY7259607.1"
                     /translation="MKKVILSLALGTFGLVMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(19411..20319)
                     /gene="mak"
                     /locus_tag="JN331_001422"
     CDS             complement(19411..20319)
                     /gene="mak"
                     /locus_tag="JN331_001422"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY7259608.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            20444..21355
                     /gene="rdgC"
                     /locus_tag="JN331_001423"
     CDS             20444..21355
                     /gene="rdgC"
                     /locus_tag="JN331_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY7259609.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(21433..21888)
                     /locus_tag="JN331_001424"
     CDS             complement(21433..21888)
                     /locus_tag="JN331_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259610.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRLIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(21885..22220)
                     /locus_tag="JN331_001425"
                     /pseudo
     CDS             complement(21885..22220)
                     /locus_tag="JN331_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(22375..22659)
                     /gene="ppnP"
                     /locus_tag="JN331_001426"
     CDS             complement(22375..22659)
                     /gene="ppnP"
                     /locus_tag="JN331_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY7259611.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(22731..23408)
                     /locus_tag="JN331_001427"
     CDS             complement(22731..23408)
                     /locus_tag="JN331_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY7259612.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIYAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(23666..23857)
                     /gene="yaiA"
                     /locus_tag="JN331_001428"
     CDS             complement(23666..23857)
                     /gene="yaiA"
                     /locus_tag="JN331_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY7259613.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRVDHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(23907..24431)
                     /gene="aroL"
                     /locus_tag="JN331_001429"
     CDS             complement(23907..24431)
                     /gene="aroL"
                     /locus_tag="JN331_001429"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY7259614.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(24614..25072)
                     /locus_tag="JN331_001430"
     CDS             complement(24614..25072)
                     /locus_tag="JN331_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY7259615.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            25192..26001
                     /gene="proC"
                     /locus_tag="JN331_001431"
     CDS             25192..26001
                     /gene="proC"
                     /locus_tag="JN331_001431"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY7259616.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(26018..27133)
                     /gene="adrA"
                     /locus_tag="JN331_001432"
                     /gene_synonym="dgcC"
     CDS             complement(26018..27133)
                     /gene="adrA"
                     /locus_tag="JN331_001432"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY7259617.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(27235..27555)
                     /gene="psiF"
                     /locus_tag="JN331_001433"
                     /pseudo
     CDS             complement(27235..27555)
                     /gene="psiF"
                     /locus_tag="JN331_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
     gene            complement(27674..29089)
                     /gene="phoA"
                     /locus_tag="JN331_001434"
                     /pseudo
     CDS             complement(27674..29089)
                     /gene="phoA"
                     /locus_tag="JN331_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(29190..29450)
                     /gene="iraP"
                     /locus_tag="JN331_001435"
     CDS             complement(29190..29450)
                     /gene="iraP"
                     /locus_tag="JN331_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY7259618.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGVLYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         7413 a         8003 c         7309 g         6924 t
ORIGIN      
        1 gtttcccaag atgcggggat tttcgcaatc tccatacatc aaaaaaaatc ccgatcttct
       61 gaccgggatt ttcaacatca acgggaactt gattaaggca gaattgacgg ctgatccgcc
      121 ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag tttcagagcc
      181 aacgcagatg ctacgtagat ggaagatgca gtgccgatgg aaacaccgat aagcatggtc
      241 agcgagaagc cttccagtac cggaccaccg aagagataca gcatcaggat aaccatcaag
      301 gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac gttaaagatt
      361 tcgtaaggcg taccgcgacg gatcttgcag aagttttcac gaattcggtc cgataccacg
      421 atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat ggtcaggtca
      481 atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg cgccagcgca
      541 ataaccaccc ctgccgccag tcgccactca aagcggaaac ctacgtacac gaggatagac
      601 agcagcgctg ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc cacgctcgga
      661 ccgacgaact caatacgctt cactgctgca ttctgattgg tggattcgtt aatcaccttc
      721 agaacttggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg catacggacc
      781 atgatgtcgt ggctgctacc aaagttttgc agcattggct cttcaaaacc ggctttttgc
      841 aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc gataaccgta
      901 ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac gatagcagcg
      961 attaacagca gaccagagat gccgaaagcc cagtagtccc agcgcataaa gtcatagact
     1021 ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca ctcctcagat
     1081 tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac gcgtaccgac
     1141 aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga accctttaat
     1201 tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga tgttcgcatc
     1261 gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa cagtacgtcc
     1321 gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga ccgctaccgc
     1381 aagggttaag acgatacccg cgatacctgg catactcagt gttgcgcctg gcaacagcga
     1441 cataataccg acgatcaaga tcaagttggc aatcagagca ctggtcgcaa tcagaccaaa
     1501 cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa ggcaagcctc
     1561 cagcccctgc tcaatgttct gcatacccag ggtcgggcca attgtacgtt cttcaacaat
     1621 ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gacagctgac gggcttcgtt
     1681 cgggttgttg atgccggtga tacggaagct gttacccaga cgagactgga tgttggcgat
     1741 gttaatcacc tcttcctgtt tcaccagaac cgcacgacca tttgcatctt tcttaccgct
     1801 gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct tggtgaagtt
     1861 agacataatg ttgccaccag cgctatcgag cgagatgtta acctgcggct ggttgtattc
     1921 gtcctggctg gaagtggagt cggtgatatg gtcaccagtc agaattacgc gtttgtacag
     1981 cacaaccggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta cgcgaccgga
     2041 tgccgcagcg gcctgatcaa cattggtgtt taccagacgg aattccagcg ttgccgtcgc
     2101 gcccagaatc tctttcgcac gcgcagtgtc ctgaatacca ggcagttcaa caacgatacg
     2161 gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta cacgattacg
     2221 caggatatta atgttctgct gcaccgcata ttcacgcgct tcactcagac gagcatcgct
     2281 cattaccgca cgcagctggt tgctgccctg gctgctaatc accaggtccg gatggcgctt
     2341 gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtga tgctcaggcc
     2401 gtagttgttt tctttacgaa cagtggtata cgggatgccc ttttcgcgca ggtcactgcg
     2461 taggctatcg atattttgtt cctggagttt gccaagcgcg gtgtccatat ccacttccat
     2521 caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag catgaatagc
     2581 tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt tgtcacccat
     2641 aacgcccatt aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga agcgcgcaag
     2701 aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt gtaacgtttt
     2761 ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag tgatctgaac
     2821 agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga caatcaccac
     2881 gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc ccttagggaa
     2941 aaattttaat tacagcgcct tcatggtgcc tttcggcagg acggcagcta cgaagtcacg
     3001 tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt tttccgctac
     3061 tttggttacg cgaccaacca ggccaccgtt cgtcagaact tcatcacctt tggcaatgga
     3121 gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga tcatgaaata
     3181 gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac cttgcgccgg
     3241 tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc cctcattatt
     3301 aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa aaatcagtta
     3361 cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc aaacgctggt
     3421 agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg caacggtcga
     3481 gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac tcaggatcga
     3541 gtgggccagt atcgctctta tatttcgcat tgcggatttt caccacgcca tcggtcacga
     3601 acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata tcgataccgc
     3661 gacgtacgcc ttcaaccagg tcttccggtt taccaacgcc catcaggtaa cgcggtttgt
     3721 ctgccggaat ttgcggacac acatgctcca gaatacggtg catatctgct ttcggctcac
     3781 ccacagccag accgccgaca gcgtagccat caaagccgat atctaccaga cctttaacag
     3841 atatatcacg taaatcttcg taaatactgc cctgaattat accaaataac gcatttttgt
     3901 taccaagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac atctccatag
     3961 agcgttttgc gtaatcccag tcagcaggat acggcgtaca ctcatcaaag atcatgacga
     4021 tatccgaacc aagatcgtac tgaatctcca tcgacttttc cgggtcgagg aaaatcgggt
     4081 cgccgttgat cgggttacgg aagtgaacgc cctgttcggt gattttacga atatcaccaa
     4141 ggctgaagac ctggaagccg ccggagtcgg taagaatcgg tcctttccac tgcataaaat
     4201 cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg gcgcagccac aggtggaagg
     4261 tgttgccgag gataatttgc gcgccagtgg cttcaacttc ttccggcgtc atccctttta
     4321 cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc cactacgcca cgatcaaaga
     4381 ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc attttttctc
     4441 cactacgtca gaaaaacagt ccaacgttta aaccagcgcc gcggaattac tccccgacgc
     4501 gctcattaat tgcctgcgga ttgtacgtga taaacatcgc atcaccgtaa ctaaaaaagc
     4561 gatatttctc ttctaccgcc gctttatagg cattcatggt gtgttgataa ccggcaaagg
     4621 ccgaaaccag cataatcagc gtcgactctg gcaagtggaa gttcgtcacc agcgcatcga
     4681 ccactttgta ctggaagccc ggatagataa agatttgggt atcgtcaaag aacggttcga
     4741 taagatcgtt ttttgccgcc tgtgccgcgc tttccagcga acgtactgaa gtggtgccaa
     4801 ccgcaatcac ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg
     4861 gtacttcagc gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct
     4921 ggaaggtgcc cgcaccaacg tgcaacgtca caaacgccat ctccacgcct ttggcgcgta
     4981 atttttccag caaaggctcg tcaaaatgca gaccagcggt cggggctgca accgcgcccg
     5041 gtttttcgct ataaacggtt tgataaagtt cgcggtcagc gtcttcgtcc ggacgaccga
     5101 tatacggcgg cagcggcata tggccgatgc tgttgaggat atccagtacc gagcgttcat
     5161 cattaaattc gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg ttgatacttt
     5221 cgtcatcgcc cagcaacagt tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg
     5281 caagaatgcg tttgtcgtcg agcatccgct caaccagcac ttcaatcttg ccgccgctgg
     5341 ctttacgccc aaacagacgc gccgggatca cgcgggtatt attaaaaacc agaagatcgc
     5401 cggggttgag cttatcgagt aaatcggtga aagtaccgtg cgtcagcgcg cccgtcggcc
     5461 cgtccagcga cagtaaacga cagctactgc gttcaggcat gggatagtgg gcaatcaggg
     5521 attcgggcaa ttcaaaggag aaatcggtaa cgcgcatgac actgactcag actaaaataa
     5581 gagtcgggta gtctagtgcc ggggcgctct ccctgcaacc tttacccccc tttggataga
     5641 atataatcga tgaatttttt agctcacctg catttagccc atctcgcgga aagctcgctt
     5701 tctggcaatt tactggctga tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac
     5761 gtcgtggctg gcattcatat gcatcgacgt atcgacgtat tgactgacaa tctgccggaa
     5821 gtccgcgaag cacgggagtg gtttcgtagt gaaacgcgcc gcgttgcgcc tattacgctg
     5881 gatgtcatgt gggatcactt tctttcccgc cactggtcgc agctgtcgcc ggactttccg
     5941 ctacaggaat ttgtctgtta tgcccgcgag caagtgatga cgattttgcc ggactcaccg
     6001 ccacgtttta tcaatctgaa caattacttg tggtcagagc agtggctggt gcgctatcgc
     6061 gatatggatt tcatccagaa cgtgttaaac ggcatggcaa gccgccgccc acgtctggat
     6121 gccctgcgtg actcctggta cgatttagac gctcattatg acgccctcga aacccgcttc
     6181 tggcagtttt atccgcggat gatggcgcag gcgtcacaca aggcgttata aaacgttagt
     6241 tcatccatac cgtagccgaa atggcaggca aagccagaat cctgtcagtc agttgcccat
     6301 gcccttcttt gcattgccat tgcacggcat tgagaaacgg tgacgcgggt agcaccactt
     6361 cacaggcctc gccacggttg attgccacca gtacacgttg ctgattcagc acgcggacaa
     6421 ataccactac gttatcttcc gcatacagca cctgacagcc gccacgacgc agcgcctgac
     6481 ttttcttacg cagcacaatc attcgccggt acagcgcgaa taacgccgta tcctgctttt
     6541 ccacctgcca ggggaacggt ttacggcaaa acggatcgtt tttaccatcc agtcccactt
     6601 catcaccgta ataaatgcac ggtacaccag gccaggtgaa cagccagacc accgccagcg
     6661 gcaggcgcgc aatatcccga ccgagcagcg ttttaaatcg cgcagtatcg tggctatcga
     6721 gctgattaaa catacgtaat tgttgttgat gagaaagccc tgcgcggtaa ttatccatcc
     6781 aggccataca ggtttgggcg tcaatttgct gcggatcata agagatgtcg gtattggcaa
     6841 gaaatcccca caacgggaat gtgaagccac gatagttcat ggcagcatct tccacatcgg
     6901 cctgtaacca ttgccgtgca tcgccaaaat gttcgccgac aatatacgcg tccggctggg
     6961 tttctttcgc cgcttcggtg atcccggcaa cgtgctgcat attattgcgc gccccacccg
     7021 cctcccccag catatgcacc acatccagcc gccagccgtc catattccac ggcgctttca
     7081 gccagtggcg gacaatactg tcttccccgc gataaatttc attcaccaga ctttccgact
     7141 gataatccag cttcggcaag ctggcatagc caagccagtc gagcgccgtg ccatcatcac
     7201 taaacgagta ccagtcgcgc cagggcgatt cagagttgtg acaagctccc cccgtgccaa
     7261 gattgtgcct gtcaaaccag gcatgggaat cgccactgtg gttaaacacg ccgtccagca
     7321 ccagccgcat tcccagctgc tgcgtattgt gtcgcaaacg cagcaacacc ccatcaccgc
     7381 caaactgcgg atcgacatgg cgataatcct cggtatcgta tttatgtacg ctgggagctt
     7441 taaacaccgg attgagatac agcgctgtca cgccaagctt tttcagatac ggcagttttt
     7501 cgcttatccc gtccagatcg ccgccataga acgttgatcc gcccgcctgc gccgtgaccg
     7561 gttcatccca gtcacgcaag atgatctctt gtccggctgc atgatggtaa tagacatgat
     7621 cctgttcagc ttcacgagga agactacgcg caaaacgatc agggaagatc tgataaaaaa
     7681 tctgatccgc agcccattgt gggccgatat ccggtacatc gacggcaaac tgctccagtc
     7741 gtgccggcgg cattcggctg aagccctgcg gtgtaaacca acgctggcga tcgtgccaca
     7801 gcagtttgaa actgtaacgc cgccggggtt gtccgctgga gagatcaatc gccgcacgcc
     7861 atgcggtgac gcctggctgc ggctgactgc gctgcttatg cattggtact gacatttctt
     7921 cgttatcgtg ttctgtacgc agcataatgc gctgcggtgg gtcttcgccc gtcagccaca
     7981 gtgtaatgag cagttgatct ttgctttgtt taacaaatgg gggcaccggc aggtgccatg
     8041 catttaacat cataattccc ctgtgatgaa aatctgatca ccttgccata gcctaatgca
     8101 ggatcactca tcatctgaag gatatttggg ggaggaggac gggggcggat aactttcgga
     8161 atagccggat caggatttcc tcctgaaccg gcttacagaa cgcaagtatt attggttttc
     8221 agccagctga cgatcgtggc ggcgtttaaa catccagcca atcaacagca caacaatcca
     8281 cgcgaaaccg acatacagcg agatacgcgt atccgggtga taaccaatca acccgataat
     8341 aaagagcagg aaaatcagcc cgccgatggt cgttgctacc ccacccggca ctttaaattt
     8401 cagcgcctta acttcttctg gcggcaaacg gcgacggaag gcgatttgcg acagcaggat
     8461 cataatccac acccataccg tggcgaaggt tgccagcgaa gcgatcacca ggaagacgtt
     8521 ttccggcatg atgtagttca gatacaccgc aaacagcagc gcggtagtca tcaccagcac
     8581 cgtaacccac ggaataccgc gacgcgacgt tttgctgaaa attttcggtg cgctgccctg
     8641 ctctgccata ccgtggagca tacggcctac gccaaatacg tcactgttaa ttgccgacag
     8701 cgaagcggtc agcacaacaa agttaagaat gctggcggca aaggtaatgc ccatatgctg
     8761 gaacgtcagc acgaacgggc taccggcagt gccaacctga ttccacgggt agatagacat
     8821 aatgacgaac agcgtaccga cgtagaacac cagaatacgc atcggcacgg agttaatcgc
     8881 acgcggaatc gatttctcag gatctttcgc ttcaccggcg gtaatcccga taatttcgat
     8941 cccaccgtaa gcaaacatca ccatttgcaa cgacattacc atgccaagcc agccgttact
     9001 gaagaagccg ccgttgctcc acaggttatg aataccggtc ggttgcccgc cgttgccaac
     9061 cccccagatg atgatgccga aaccggcgac aatcatgatg atgatggtgg cgactttaaa
     9121 gaacgagaac cagaattcca gctcaccgaa gaccttcacg ctcatcaggt ttacggcgca
     9181 aatgatcagc accacgctca gtacccaaat ccagtgcggc accgtcggga acaagacacc
     9241 catatagata ccaaaagcgg tcacatcggc gatggcgaca ataaggattt caaagcagta
     9301 ggtccatccg gtaatgtaac ctgccagcgg gccaaggttt tcctgcgcat aacgcgagaa
     9361 agagctggcg gccgggttat gtaccgacat ttcccccagc gcacgcataa tgatatacgc
     9421 cgcgatacca ccgataatat aggccaacaa cacgctcgga ccggccattt tgatggcgtc
     9481 tgccgaaccg taaaacagcc cggtgccaat tgctgaaccc agtgccataa agcgtatgtg
     9541 gcgggtactt agcccacgct ttagcttatt cttactttcc atcaattcct aacccatcaa
     9601 cacaataaaa aaccacgggg catcgcagcc ccgtggttaa aacaaatgtt cagtgattta
     9661 gtgagcgctg gaggtcacct gacgacctgc cgcgcgatcc cagataatgg ccagaaccac
     9721 catcaccact gttggcatta accacgccag accttgttcg gccagcggta aacgctgcgc
     9781 ccaggacggt aagatatcgc tgaatgcaga tgctttgatc ccgtcgagaa taccaaaaag
     9841 caggctgata aacatcggcg gagcaatcac gcgggacgaa ttatgccacc atgagcgtgt
     9901 aaaacttaat acaaccagtg cgatacacgg cggataaata gcggtcagca ccggtacgga
     9961 gatctggatc agctggctta agccgaggtt agacaccacc atcgagaagc cgccgaggat
    10021 aaacaccagc gtacgataag agagcggtac gtactgggca aagaattctg cacaagcaca
    10081 ggtcaggcca accgccgtga ccagacaggc gatgaagatt aacgccgcca gcaggaagct
    10141 accgccgccg ccaaaggtgt gctgaacgta agcatgcaga atagcagcgc cgtttgcaga
    10201 ctgatcgacc agcgacgcgc tgtctgaacc cagacagaac agcgccaggt acagcaaagt
    10261 cagaccaaca cccgccatca ggccagccca gacggtataa cgggtcagca gacgcgcttc
    10321 ggtaacgcca cgagaacgcg ccgcgttaac aataacgata ccaaacacca ttgcgcccag
    10381 cgtatccatg gtcagatagc cgttaacgaa gccgttagaa aacgcagcgt tttgataagc
    10441 ctcagtcgcc gtgctgatag aacccgccgg ccagacaatc gcggcaacag acaggatgac
    10501 cagcgcgata attttcagcg gcgcaaggaa gttgcccacg gtatccagca gcttgcccgg
    10561 atagagcgaa accagaataa cgatagcgaa atagaccagg ctgtagataa acagcggcag
    10621 cgcggaatca cccgtcagcg gcgcaatccc cacttcaaag gaaacggtag ctgtacgcgg
    10681 cgtagcgaaa agcggcccca ccgccaggta acaaaccgtt gccagcagta cgccagcgac
    10741 tttaccgatt ggcgtgctga ggctgtcaac accgccgcca acttttgcca gcgccactac
    10801 cgttaatacc ggcaggccaa cggcagtaat gaggaagccg aatgccgcag tccagacgtg
    10861 ttcgcctgcc tgtaagccga ccattggagg gaaaataatg ttacctgcgc cgacgaacaa
    10921 cgcaaatgtc ataaagccca gagcgatgat atcgcgcgat cttaattgat gggtcataaa
    10981 tcatactgcc tgtggatgtg gtgttgtaac gttaaatttt tgtgccaatc cctggggatg
    11041 atacagcggt atatttaaac aattttagcg ggaaatactc ccaaccaggc gcggcgaaga
    11101 atagtttttc gatttaccgc gttaatgcag tcagtatgac agcacctgaa gcgcaattta
    11161 aacgcttata acgttttaaa gcaaggaggg atctaaaaac cagaggtata tagcgataag
    11221 aaatcaccaa ccagtataaa acactatttc cgtgaaaaag acatggctaa tcatgcgaac
    11281 aaaaaatcac caaccgttgt aaaatctaac gcccgtattt tgactgcaaa gaaaataagc
    11341 cagcatcggc tggcttatgg caataaaaga tgacaaaggc ggattaatcg ctgtttttgg
    11401 caattaaacg ttccgggata acaaaactga aacgtgttcc ttttcctact gtactctcaa
    11461 tattcaggcg actttcgtga tgattcaccg catgtttcac gatcgctaac cctaatccgc
    11521 taccgccggt ttgccgggaa cgcgctttat caacgcgata aaaacgctcg gtcaggcgcg
    11581 gaatatgctc cggtgcaatg cccggtccgt tatcttcaac gctaaattcg gcaccgtgcg
    11641 gcactcgctg ccagcgtacg gtgatatgcg tgccttccgg cgtatgattc acggcgttat
    11701 agaccaggtt cgaaatcgca ctgcgtagct gatcttcgtt gccagacacc ttgaggccgt
    11761 tatctatctc aaaggtaaat gtctgttttt tctgactcag agtctgagcc tcgcgctcaa
    11821 caacgcgcag catcatcggc acatcaacct tttcattgag caattgcgtc ggcgcggctt
    11881 caattttcga cagcgttagc aactgcttca ccagcccttc catccgctgg gtctgctcgc
    11941 gcatggtgtg caacgctttt tcgcgtaccg cgccttccag cggctgctca tccatcatct
    12001 ccaggtaacc ctgtaacacg gtcaatggcg tacgtaactc atggctcacg ttggcaaaaa
    12061 agttacgccg cgccccttcc agttgatgca tttgcgtgac atcacgcgcc accatcagca
    12121 actgtttgtg ggtataaggc atgacgcgaa tttccagatg ccgcccggtg ttgagcacca
    12181 gattgagcgg gcgagaaaaa tcacgcgttt tcagatattg cgtaaactcc gggtaacgca
    12241 gtaggttaag gatgttctgc ccgttatctt ccggccagcg caaaccaaga atttgttgcg
    12301 ccagaccgtt acaccagaaa ataccgccct cttccgtggt cagcaccacc gcgtcgggca
    12361 gcgactccgc gccgctacga aagcgtttaa tcagattgcc cagttcacgg cggcgttttt
    12421 tatttcgcag ctgcatctgg tgtaagccgt atagtagcgg ttcccagcta ccacgccccg
    12481 gtggcggggt catactgcga tccacccaca gccaccatga aaggcgcaat aaattccaga
    12541 aatgccagat aagcagtcct gttaccgatg ccagcaaaaa ccagggcagg taaccaaaaa
    12601 atgcacccag gatgaaagcc gggaggcagc aaagtagcag ctccagcacc agccttttcc
    12661 acgacagccg ttccagcacg taagatactc cagttaagaa atcataagcc ctgctctgcg
    12721 tccgatgagc aaggcgttaa aagcgggttg aaaaacgata tcctgtaccg cgcacggtct
    12781 gcaccatgcg gtcatgcccg ccgggctcca gtgctttacg caggcgacga atgtggacat
    12841 cgaccgtgcg gtcttccaca tacacgttag ttccccagac gtggtttaac agctgctcgc
    12901 ggctgtacac gcgctcagga tgcgtcataa aaaagtgcag cagtttaaat tctgtcggcc
    12961 ccatctccag cggctcttcg cccgccatca ctcggtgaga tgtcgggtcg agacttaatc
    13021 cctgcatctc aatcacctct tccaccgcca ttggcgaaat acggcgcatt accgctttga
    13081 ttcgcgccac cagctccttc ggcgaaaatg gcttggtgat atagtcatcc gcgccggttt
    13141 caaggccgcg cacgcgatct tcttcttccc ctctggcggt caacatcacc actggaatat
    13201 cccgggtcat cgactcgcgc ttgaggtgtt tgatgaactg gataccggag ccgccaggta
    13261 acatccagtc aaggagaatt aaatccggcc agggttcatt cagttgattc acagcactgt
    13321 cataatcttc cgcttcgacc ggctgaaagc cattttgttc gagcacgaag cagaccattt
    13381 cgcgaattgg agcttcatct tctacgacca gaatacgtct cgccatgatt tgccctgttg
    13441 taataaatag gttgcgatca ttaatgcgac gtcattatgc gtcagattta tgacagattt
    13501 atgaaaagct cgtcgcacat atcttcaggt tattgatttc cgtggcgcag aaaaaagcaa
    13561 atggcacatc tgtttgggta taatcgcgac catgcttttt cgccagggaa ccgttatgcg
    13621 catccttcac acctcagact ggcatctcgg ccagaacttc tacagtaaaa gccgcgaagc
    13681 tgaacatcag gcttttcttg actggctgct ggagacagca caaacccatc aggtggatgc
    13741 gattattgtt gctggtgatg ttttcgatac cggctcgccg cccagttacg cccgcacgtt
    13801 atacaaccgt tttgttgtca atttacagca aactggctgt catctggtgg tactggcagg
    13861 aaaccatgac tcggtcgcca cgctgaatga atcgcgcgat atcatggcgt tcctcaatac
    13921 aaccgtggtc gccagcgccg gacatgcgcc gcaaatcttg cctcgtcgcg acgggacgcc
    13981 aggcgcagtg ctgtgcccca ttccgttttt acgcccgcgt gacattattt ccagccaggc
    14041 ggggcttaac ggtattgaaa aacagcagca tttactggca gcgattaccg attattacca
    14101 acaacactat gccgatgcct gcaaactgcg cggcgatcag cctctgccca tcatcgccac
    14161 gggacattta acgaccgtgg gtgccagtaa aagtgacgcc gtgcgtgaca tttatattgg
    14221 cacgctggac gcgtttccgg cacaaaactt tccaccagcc gactacatcg cgctcgggca
    14281 tattcaccgc gcacagatta ttggcggcat ggaacatgtt cgctattgcg gttcccccat
    14341 tccactgagt tttgatgaat gcggtaagag taaatatgtc catctggtga cattttcaaa
    14401 cggcaaatta gagagcgtgg aaaacctgaa cgtaccggta actcaaccca tggcggtgct
    14461 gaaaggcgat ctggcgtcga ttaccgcaca gctggaacag tggcgcgatg tatcgcagga
    14521 gccacctgtc tggctggata tcgaaatcac tactgatgag tatctgcatg atattcagcg
    14581 caaaatccag gcattaaccg aatcattgcc tgtcgaagta ttgctggtac gtcggagtcg
    14641 tgaacagcgc gagcgtgtgt tagccagcca acagcgtgaa accctcagcg aactcagcgt
    14701 cgaagaggtg ttcaatcgcc gtctggcact ggaagaactg gatgaatcgc agcagcaacg
    14761 tctgcagcat cttttcacca cgacgttgca taccctcgcc ggagaacacg aagcatgaaa
    14821 attctcagcc tgcgcctgaa aaacctgaac tcattaaaag gcgaatggaa gattgatttc
    14881 acccgcgagc cgttcgccag caacgggctg tttgccatta ccggcccaac cggtgcgggg
    14941 aaaaccaccc tgctggacgc catttgtctg gcgctgtatc acgaaactcc gcgtctctct
    15001 aacgtttcac aatcgcaaaa tgatctcatg acccgcgata ccgctgaatg tctggcggag
    15061 gtggagtttg aagtgaaagg tgaagcgtat cgcgcgttct ggagccagaa tcgggcgcgt
    15121 aaccagccgg acggtaattt gcaggtgcct cgcgtagagc tggcgcgctg cgccgacggc
    15181 aaaattctcg ccgacaaagt gaaagataag ctggaactga cagcgacgtt aaccgggctg
    15241 gattacgggc gcttcacccg ttcgatgctg ctttcgcagg ggcaatttgc tgccttcctg
    15301 aatgccaaac ccaaagaacg cgcggaattg ctcgaggagt taaccggcac tgaaatctac
    15361 gggcaaatct cggcgatggt ttttgagcag cacaaatcgg cccgcactga gctggagaag
    15421 ctgcaagcgc aggccagcgg cgtcacgttg ctcacgccgg aacaagtgca atcgctgaca
    15481 gcgagtttgc aggtacttac tgacgaagaa aaacagttaa ttaccgcgca gcagcaagaa
    15541 caacaatcgc taaactggtt aacgcgtcag gacgaattgc agcaagaagc cagccgccgt
    15601 cagcaggcct tgcaacaggc gttagccgaa gaagaaaaag cgcaacctca actggcggcg
    15661 cttagtctgg cacaaccggc acgaaatctt cgtccacact gggaacgcat cgcagaacac
    15721 agcgcggcgc tggcgcatat tcgccagcag attgaagaag taaatactcg cttacagagc
    15781 acaatggcgc ttcgcgcgag cattcgccac cacgcggcga agcagtcagc agaattacag
    15841 cagcagcaac aaagcctgaa tacctggtta caggaacacg accgcttccg tcagtggaac
    15901 aacgaactgg cgggttggcg tgcgcagttc tcccaacaaa ccagcgatcg cgagcatctg
    15961 cggcaatggc agcaacagtt aacccatgct gagcaaaaac ttaatgcgct tgcggcgatc
    16021 acgttgacgt taaccgccga tgaagttgct accgccctgg cgcaacatgc cgagcaacgc
    16081 ccactgcgtc agcgcctggt cgcgctgcat gggcagattg ttccccaaca aaaacgtctg
    16141 gcgcagttaa tggtcactat ccagaatgtc actctggaac agacgcaacg taatgtcgca
    16201 cttaacgaaa tgcgccagcg ttataaagaa aagacgcagc aacttgccga tgtgaaaacc
    16261 atttgcgagc aggaagcgcg catcaaaacg ctggaagccc agcgtgcaca gttacaggcg
    16321 ggtcagcctt gtccactttg tggttccacc agccacccgg cggtcgaggc gtatcaggcg
    16381 ctggagcctg gtgttaatca gtctcgatta ctggcgctgg aaaacgaagt taaaaagctc
    16441 ggcgaagaag gtgcggcgct gcgcgggcaa ctggatgcat taacgaagca gcttcagcgc
    16501 gatgaaaacg aagcgcaaag cctccgacaa gatgagcaag cacttactca acaatggcaa
    16561 gccgtcacgg ccagcctcaa tatcaccttg cagccacagg acgatattca accgtggctg
    16621 gatgcacaag atgagcacga acgccagctg cggttactca gccaacgaca tgaattacaa
    16681 gggcagattg ccgcgcataa tcagcaaatt atccagtatc aacagcaaat tgaacaacgt
    16741 cagcagcagc tcttaacggc attggcgggt tatgcactga cattgccaca ggaagatgaa
    16801 gaagagagct ggttggcgac acgtcagcaa gaagcgcaga gctggcagca acgccagaac
    16861 gaattaacca cgctgcaaaa ccgtattcag cagctgacgc cgattctgga aacgttgccg
    16921 caaagtgatg atctcccgca cagcgaagaa actgtggcgc tggataactg gcggcaggtt
    16981 catgaacaat gtctcgcatt acacagccag cagcagacgt tacagcaaca ggatgttctg
    17041 gcggcgcaaa gtctgcaaaa agcccaagcg cagtttgaca ccgcgctaca ggccagcgtc
    17101 tttgacgatc agcaggcgtt ccttgcggcg ctaatggatg aacaaacact aacgcagctg
    17161 gaacagctca agcagaatct ggaaaaccag cgccgtcagg cgcaaacgct ggtcactcag
    17221 acagcagaaa cgctggcaca gcatcaacaa caccgacctg acgggttggc tctcactgtg
    17281 acggtggagc agattcagca agagttagcg caaactcacc aaaagttgcg tgaaaacacc
    17341 acgagtcaag gcgagattcg ccagcagctg aagcaggatg cagataaccg tcagcaacaa
    17401 caaaccttaa tgcagcaaat tgctcaaatg acgcagcagg ttgaggactg gggatatctg
    17461 aattcgctaa taggttccaa agagggcgat aaattccgca agtttgccca ggggctgacg
    17521 ctggataatt tagtccatct cgctaatcag caacttaccc ggctgcacgg gcgctatctg
    17581 ttacagcgca aagccagcga ggcgctggaa gtcgaggttg ttgatacctg gcaggcagat
    17641 gcggtacgcg atacccgtac cctttccggc ggcgaaagtt tcctcgttag tctggcgctg
    17701 gcgctggcgc tttcggatct ggtcagccat aaaacacgta ttgactcgct gttccttgat
    17761 gaaggttttg gcacgctgga tagcgaaacg ctggataccg cccttgatgc gctggatgcc
    17821 ctgaacgcca gtggcaaaac catcggtgtg attagccacg tagaagcgat gaaagagcgt
    17881 attccggtgc agatcaaagt gaaaaagatc aacggcctgg gctacagcaa actggaaagt
    17941 acgtttgcag tgaaataact attcagcagg ataatgaata cagaggggcg aattatctct
    18001 tagccttgct ggtcgttatc ctgcaagcta tcactttatt ggctacggtg attggtagcc
    18061 gttctggtgg ttgtgatggt ggtatgaaaa aagtcatttt atctttggct ctgggcacgt
    18121 ttggtttggt gatggccgaa tttggcatta tgggcgtgct cacggagctg gcgcataacg
    18181 taggaatttc gattcctgct gccgggcata tgatctcgta ttatgcactg ggggtggtgg
    18241 tcggtgcgcc aatcatcgca ctcttttcca gccgctactc actcaaacat atcttgttgt
    18301 ttctggtggc gttgtgcgtc attggcaacg ccatgttcac gctctcttcg tcttacctga
    18361 tgctcgccat tggtcggctg gtatccggct ttccgcatgg cgcatttttt ggcgtcggag
    18421 cgatcgtgtt atcaaaaatt atcaaacccg gaaaagtcac cgccgccgtg gcggggatgg
    18481 tttccgggat gacagtcgcc aatttgctgg gcattccgct gggaacgtat ttaagtcagg
    18541 aatttagctg gcgttacacc tttttattga tcgctgtttt taatattgcg gtgatggcat
    18601 cggtctattt ttgggtgcca gatattcgcg acgaggcgaa aggaaatctg cgcgaacaat
    18661 ttcacttttt gcgcagcccg gccccgtggt taattttcgc cgccacgatg tttggcaacg
    18721 caggtgtgtt tgcctggttc agctacgtaa agccatacat gatgtttatt tccggttttt
    18781 cggaaacggc gatgaccttt attatgatgt tagttgggct agggatggtg ctgggaaata
    18841 tgctaagtgg caggatttca ggacgttatt caccactgcg cattgcagca gtgactgact
    18901 ttatcattgt actggcactg ctgatgctct ttttctgcgg cggcatgaaa acaacgtcgc
    18961 ttatttttgc ttttatttgt tgcgcgggat tatttgccct ttcagcacct ctgcaaatat
    19021 tgttactgca aaatgccaaa ggcggagagt tattaggtgc cgcaggtggg caaatagcgt
    19081 ttaacctcgg tagcgccgtc ggcgcatatt gcggaggtat gatgctgacg ctggggctgg
    19141 catataatta cgtggcgctg cctgccgccc tgctttcgtt tgctgcgatg tcatcgttgc
    19201 tgctgtatgg tcgctataag cgccagcaag cggcggatag tccggtgctg gcgaaaccac
    19261 tggggtagat tactgtcacg gtgcgcgttg cttattgccg gatgcggcgt gaacgcctta
    19321 tccgccctac gcggttctgg cacattttgt aggcctgata agacgcggca agcgtcgcat
    19381 caggcatgat gcgccaattg cctacgtttt ttactcttgt ggccataacc acgcagcgcc
    19441 gcgtacgccg ctggaatcac cgtgcttcgc cttacgcacc ggcgtttcac attcgccgcc
    19501 gaagacaaat tgtttaatca actgcccaac cgtttgatat aaacggtcta cattgctcat
    19561 cccgcccccc aggacaatca catccggatc gagaatattc acgacatgtg ccagcgattt
    19621 tgccagccgc agctcgtagc gacgcaatgc cagttccgct accggatcgc tttcttcaac
    19681 caggcggata atttcactgc ctttcagcgc atgtccgctc aaacgacgat aatccgtcgc
    19741 gaatcccgtg cccgaaataa aggtttcaat acaaccttgt ttaccgcaat aacaagggac
    19801 ttcctcgcga taacgcagtt cgtcttcgtc catccacggt agcggattgt gtccccactc
    19861 acctgccgtg ccattgccgc cgatatgcgt ccgcccattg aatgccacgc ccgcgccgca
    19921 tcccgtgccg ataatcacgg caaataccgt ctgcgctccc gctgccgcgc catctactgc
    19981 ttctgaaacc gccagacagt tggcatcatt tgccagccgc acttcccgct gcaacctcgc
    20041 gcttaagtct ttatcgaatg gctgaccgtt gagccaggtt gaattggcat tcttcaccac
    20101 accggtgtag ggcgaaatcg aacctggaat gcccatacct accgttccgc gctgccctgt
    20161 cgcctgctcc gccatatcaa ccaacgtggc gatcgtttca atagtctgcc ggtaatcatc
    20221 acgcggcgtg ggcagacgat ggcggtacaa ctgctcccct gcatcgccca gtgcaatcac
    20281 ttcagttttg gtgccgccta aatcgatacc tatacgcact gtactctcct tatttttttc
    20341 aatatcaata gcgtagagac ggacaaccgg attggcaatg caaggccgcc gacaattcgt
    20401 tatcatgccc gctaaattta acgacaaggc cgtggaaatt atcatgctgt ggttcaaaaa
    20461 tttaatggtt taccgtctta gccgcgagat ttcgctgcgt gcagaagaga tggaaaaaca
    20521 gctagcctcg atggcattta ccccatgcgg cagccaggac atggcgaaga tgggctgggt
    20581 tcctccgatg ggatcgcaca gcgatgcgtt aacgcacgtt gccaatggtc aaattgttat
    20641 ctgcgcgcgc aaagaagaaa aaatcctccc gtctccggtg attaaacagg cgctggaagc
    20701 gaaaatcgcc aaactggaag cggaacaggc gcgtaagctg aagaaaaccg aaaaagattc
    20761 gctgaaagac gaagtgctgc actctctgct gccgcgtgct ttcagccgtt ttagccagac
    20821 aatgatgtgg ctcgacacgg ttaacggttt gattatggtg gactgcgcca gtgccaaaaa
    20881 agcggaagat acgctggcat tactgcgtaa aagcctgggg tcgttaccgg ttgtaccatt
    20941 gagcatggaa aacccgattg aactgacgct gaccgaatgg gttcgctccg gtagtgcggc
    21001 acagggcttc cagctgcttg atgaagccga gctgaaatcg ttgctggaag atggcggcgt
    21061 gatccgcgcg aagaaacaag atctgaccag cgaagagatc accaatcaca ttgaagccgg
    21121 aaaagtggtg actaaactgg cgctcgactg gcagcagcgc attcagtttg tgatgtgcga
    21181 cgatggttcg ctcaagcgtc ttaagttctg cgacgagctg cgcgatcaaa acgaagatat
    21241 cgaccgtgaa gatttcgccc agcgttttga tgccgatttc atcctgatga ctggtgaact
    21301 ggcagcgtta attcaaaacc tgattgaagg attaggtggc gaagcacaac gttaattgct
    21361 gattttcctt taatgccgga tgcgacgcct gccgcgtctt atccggcgta cgaagccaca
    21421 ccaggcatat aattattcgc tacggcgagc aataattttt agcgcagcaa tattatgcgt
    21481 tttacgccgt aactgatcta atcgcctcat ccctattagc ctgtcagcag cacgcagaat
    21541 attcactaac ccccatttat cgctggtaaa ttgttttgtt aacagctccg cttttggggt
    21601 atcaatatat tcacgcaata aatcagaaat tgaacaaata tccgtcaggt aaggatacca
    21661 gcaagtttca ccgtgaaaat tacgcactcc agcgttacct gcaataaagt ctttcgggta
    21721 atcccagatc acgtttttat ccaacgtgat ccaataacgc ggtaaatcgg tacttccacc
    21781 attttggctg cgcatgggat aacgggtaca gtgaatttgc aggttgatcg ttggcgtcag
    21841 taagtcatag atctcccgct gtaacttgct ccatggacgt tgtgtcattg tttttcctca
    21901 agccagtgac gcgcttcact gcgaattttc cgcgttgccc cggttttcac taaatcacgt
    21961 aactgcgaaa gatcgggttg ttcgacaaat aagagtaatg atggttcctc ttttaaccac
    22021 gccgttttca catcggcagc cagctgtgga ttattaatag ccagcgaccg atcctgaggc
    22081 tcagtcaatg tcgttaacaa cgacgccggg gtatgtgcgt tgcgtaatgc cgtttggcgc
    22141 aaatgatcat tgcgactttg cagcaatgca cgccaccatt catcatctga tttatctttt
    22201 atcgtagcca gttccgcaaa aaatacctgg agatcttcac tgatatttat atcaaccttg
    22261 atgaattatg aatatatcgg aaattgaaaa aatcgcagca aaggaaatat tattatgggt
    22321 taaagagagg atatttcaga aaaatgccct cccgttcaag gggaaggcga ggaattacag
    22381 atagcggcac agataagagg tgggttcggc aacttgaaga tgaaactcac tgtgaccggg
    22441 aacattaaac accgaaccgg cttcatacac ttgccagtcg gtcgcgtcag gcagtaacac
    22501 attcagcgcg ccactgatta ccgtcatctc ttccggctca gcggtgctga aggtgtattc
    22561 gccttcaacc ataacaccca cgctggcgcg accagtgctg ctgctggaaa agccgatcga
    22621 tttcactttg ccggaaaagt actcattact ttgaagcata aactggccct gtctggtagt
    22681 tgaacatcta cttcactata ggggccagag gcgctggctg tcacgcaaaa ttacaccagt
    22741 aattccgcag ccagccgtgc aatcaatacg ttagagagca agacaggaac atcgagctgt
    22801 ttttgcagta aatcacgatg acgttggtga aatcctaaac aatccagcat gatgacatca
    22861 gccccttttg ccagtaattc tttcccagca taaatgattt tttgttctga atcatgaatg
    22921 gggttaccca atgaaaatac cggcgatttc tgcaaaattt gccatttttg cgcctgaacg
    22981 ggcagcatct cctcaaccgg aacgataacc cccacctgat gatcttcaac aatagaggaa
    23041 accagtggag gcaatattcg cgacggctca agaaagatcg tattacgcgc agtcatacta
    23101 ctaatgtttg ctgtactcat taataaaatg acgtcataac cctgattatc gagcacttca
    23161 accacacctt gcagatctcg ctccacttta cgacgcgaaa catgggccag ctggttgtca
    23221 tttaataatg tgagaatggt gtcttcgcct gcttctggcg cgtactccgc catcacttct
    23281 tcacgactta acttccccag caggctatga tgggaaatat tatcttcgtc aatgtattcc
    23341 gtcaggagcg gcaaaacctc ctgcataggt acaatgccga tggtcaggat cgccaacgac
    23401 gcactcattg ttcatccacc ttacttttct ttcgtcgtta attaccgggc aagtgtgaag
    23461 caccactgct gacacttact tgtgctacaa atgacaaaaa gcgtagcagc agacgtgcac
    23521 ggcataatgt aaagattcca aatgattcca gtaatggaat gttattgttt aatattctaa
    23581 ttattagaaa aacatgaatt atgaaaaatg tgacgcagat cccttgtagt ggcactcaga
    23641 atcccttccg agcagtctga tgcggtcact ctttgtcagg gtcctcatag cgttttttcg
    23701 catccatcgc ttcctgagcc gtcggatgtt cactgatcaa cgagtctggt ttaggatgat
    23761 cgactctgag ttggtaccag gttaccgtct gtcctggctt tcctttttca atcgtcacta
    23821 tatatgcttc acgaggataa ggcggtttcg ttggcatcgt tcttccttat ttcacgggat
    23881 gaacgttaag tataggcgct ctaaaatcaa caattgatcg tctgtgccag ggcgctacga
    23941 atttcagaaa tcacctggct gggttcgttt gttgcgtcga tgataatatg cgcaacttcg
    24001 cgatatagcg catcgcgttc ttccagcact tcctgaactt cttcgctcag cggttttccc
    24061 gttaaggttg gccgtaaatc ttcttccggt gcagcttgca gtcggttaac caggactgat
    24121 actggcgcac acaaataaac cacgatcccg ttattttgca tgaagtgacg attaaattcc
    24181 gtcagaataa tgccgccgcc tgtagcgata acggtggatg gcgcagttac cgcttccagc
    24241 gccgccgttt ctctggcgcg aaatcccgcc cactcttccc tttcgacgat ctccgcgacc
    24301 gtcatattga gctgtgattg caaccactga tcggtatcga caaaccgacg gttaagcgaa
    24361 tcggcaaggg ccattccgac cgttgtttta ccacagcccc gaggcccgat cagaaaaaga
    24421 ggttgtgtca tcgtgggttt tccccaatag gtcgcaatgc ggcgaaagcc ggtgtcatga
    24481 gaatagcgat cataccatca aactagtaca atttcgattg taaagaaaaa attccactta
    24541 aagtgaaaat ctcaatacat cccttactat accaataaat attcaagaat gaagtgtaaa
    24601 taataaatta catttagcca cgactacgtt gcacttccag ccaccacttc tccagctccg
    24661 cggcaaaggc ctggcggtca cgttgtgaaa ggctatctgg tccgccggtc tggatcccac
    24721 tggcacgtaa ggtatccata aaatcgcgca tcgtcaggcg ctcacgaatg gtcgctggcg
    24781 tgtaacgttc gccgcgcgga ttaagcgccg ctgcgccttt ctcgatggct tcagcagcca
    24841 aaggtatatc tgcggtgatc accaaatcgc ccgcttcaca ctgccggaca atttcgttat
    24901 cggcaacgtc gaaacctgcc gcgacgcgca gcgtacgaat aaatcgcgat ggcggcacgc
    24961 gtaaactctg gtttgctacc agtaccagcg gcatctgcat acgttccgcc gcgcgataca
    25021 aaatctcttt aattacattg ggacacgcgt cggcatccac ccaaattgtc ataaagtcat
    25081 cctttgttgg gtaatcctct attgtgtcgc gcttttgcct tccggcatag ttctgtttat
    25141 gcttctgcca gcgattatca aaacaatgaa tttcacggca ggagtgaggc aatggaaaag
    25201 aaaatcggtt ttattggctg cggcaatatg ggaaaagcca ttctcggcgg tctgattgcc
    25261 agcggtcagg tgcttccagg gcaaatctgg gtatacaccc cctccccgga taaagtcgcc
    25321 gccctgcatg accagttcgg catcaacgcc gcagaatcgg cgcaagaagt ggcgcaaatc
    25381 gccgacatca tttttgctgc cgttaaacct ggcatcatga ttaaagtgct tagcgaaatc
    25441 acctccagcc tgaataaaga ctctctggtc gtttctattg ctgcaggtgt cacgctcgac
    25501 cagcttgccc gcgcgctggg ccatgaccgg aaaattatcc gcgccatgcc gaacactccc
    25561 gcgctggtta atgccgggat gacctccgta acgccaaacg cgctggtaac cccagaagat
    25621 accgctgatg tgctgaatat tttccgctgc tttggcgaag cggaagtaat tgctgagccg
    25681 atgatccacc cggtggtcgg tgtgagcggt tcttcgccag cctacgtatt tatgtttatc
    25741 gaagcgatgg ccgacgccgc cgtgctgggc gggatgccac gcgcccaggc gtataaattt
    25801 gccgctcagg cggtaatggg ttccgcaaaa atggtgctgg aaacgggaga acatccgggg
    25861 gcactgaaag atatggtctg ctcaccggga ggcaccacca ttgaagcggt acgcgtactg
    25921 gaagagaaag gcttccgtgc cgcagtgatc gaagcgatga cgaagtgtat ggaaaaatca
    25981 gaaaaactca gcaaatcctg atgactttcg ccggacgtca ggccgccact tcggtgcggt
    26041 tacgtccggc tttctttgct ttgtaaagcg ccaaatctgc cgatttcaac cactcacgat
    26101 agtgactcat ttgtgggttc agcggcgcaa cccccacact aatccgtaaa gttacctgtg
    26161 gcgtattcgg caagcgtaac gtgtttagcc cttcacgcac ccgtaacatg gcggtaatgg
    26221 cgctctcagc tggcgtaccg gacatgatta ctgcaaactc atcgccgcca aaccgaccaa
    26281 tcacatcgct accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat
    26341 cgcccacatc atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca
    26401 gtaacgttgc atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt
    26461 cccaatgacg tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc
    26521 tgcgtttatg ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag
    26581 gataaacgac aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca
    26641 cggtaatgcc cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg
    26701 cgacaaacag acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag
    26761 cggtggaagg cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat
    26821 cggtttttaa gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc
    26881 aagctaaatg cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg
    26941 gcgggtgcga aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac
    27001 ggggtagtct gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag
    27061 ttaaaggagg ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta
    27121 tttttgggaa cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag
    27181 tatgaaattt cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc
    27241 ggctttcttg aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg
    27301 cgttgcttga ttattacatt cgcgcatctt ttgctactgt ggcgtcaaac ttttttcacc
    27361 aggcgcagac ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt
    27421 caacgcctgc gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa
    27481 ggttctctcg gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag
    27541 taatgttatt ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt
    27601 gatctgccat taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact
    27661 gccgggcgcg gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct
    27721 ggtcggtcag tccaacaaca ttggcggcat gcgggccata cgccgcaata cgcaactgac
    27781 tgccggtatg ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc
    27841 catctttagt atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct
    27901 ggctggcgtg ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga
    27961 attccagcgc ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag
    28021 gattcgcagc gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt
    28081 tctcattttt actcaacaat tcaatggctt tgtcggtcat ctgtgccagg gttggtacac
    28141 tgtcattacg ttgcggatta ggcgtatagg tgactgcggg cttatcgata ttgccgtggt
    28201 acgttgcttt cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca
    28261 ggggtttttg ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact
    28321 gataaccacg cgcctgtgcc tattcacgca gcgtttttcc ctgccattca ccagcggttg
    28381 ccgtttcagc aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca
    28441 gctgttcggt aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac
    28501 tggtcgcgct cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg
    28561 tggcatcctg caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca
    28621 tttccagaat cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg
    28681 ttttgacacc ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt
    28741 tgccagtttt tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga
    28801 tacctttaaa aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat
    28861 cccccatccc atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat
    28921 cacgcagagc ggcggtctga tcacccgtta agcggcgagc accgccgggt gcagtaatat
    28981 cgccctgagc agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag
    29041 gggtaaacag taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc
    29101 catgtacaaa tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact
    29161 ttatgacagc tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt
    29221 acgtaatcgc gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg
    29281 tacagcaccc cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga
    29341 agcattgcag tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct
    29401 tcttcttttt gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt
    29461 ccttatccaa agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt
    29521 attattttta tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa
    29581 acgtttctct tatttaaata attacccaca caaaatatag gcttttaatt atcaaccttt
    29641 attataaat
//
LOCUS       DADLSB010000032        29129 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.032, whole genome shotgun sequence.
ACCESSION   DADLSB010000032 DADLSB010000000
VERSION     DADLSB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.032"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(1..846)
                     /gene="gsiB"
                     /locus_tag="JN331_001436"
                     /pseudo
     CDS             complement(1..846)
                     /gene="gsiB"
                     /locus_tag="JN331_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
     gene            complement(866..2737)
                     /gene="gsiA"
                     /locus_tag="JN331_001437"
     CDS             complement(866..2737)
                     /gene="gsiA"
                     /locus_tag="JN331_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY7259619.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQDPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEYAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCEVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(2724..3689)
                     /gene="iaaA"
                     /locus_tag="JN331_001438"
     CDS             complement(2724..3689)
                     /gene="iaaA"
                     /locus_tag="JN331_001438"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7259620.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            3893..5128
                     /gene="moeA"
                     /locus_tag="JN331_001439"
     CDS             3893..5128
                     /gene="moeA"
                     /locus_tag="JN331_001439"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY7259621.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            5128..5877
                     /gene="moeB"
                     /locus_tag="JN331_001440"
     CDS             5128..5877
                     /gene="moeB"
                     /locus_tag="JN331_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY7259622.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(6056..6718)
                     /gene="fsa"
                     /locus_tag="JN331_001441"
     CDS             complement(6056..6718)
                     /gene="fsa"
                     /locus_tag="JN331_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7259623.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            6849..7748
                     /locus_tag="JN331_001442"
     CDS             6849..7748
                     /locus_tag="JN331_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY7259624.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLPAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            7754..10186
                     /locus_tag="JN331_001443"
     CDS             7754..10186
                     /locus_tag="JN331_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY7259625.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            10332..11147
                     /gene="ybiV"
                     /locus_tag="JN331_001444"
     CDS             10332..11147
                     /gene="ybiV"
                     /locus_tag="JN331_001444"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY7259626.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            11299..12564
                     /locus_tag="JN331_001445"
     CDS             11299..12564
                     /locus_tag="JN331_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY7259627.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPNRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(12805..14397)
                     /locus_tag="JN331_001446"
     CDS             complement(12805..14397)
                     /locus_tag="JN331_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY7259628.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            14616..15536
                     /gene="ldtB"
                     /locus_tag="JN331_001447"
     CDS             14616..15536
                     /gene="ldtB"
                     /locus_tag="JN331_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7259629.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDLVVLDEAIKNRSGMPVRLN"
     gene            complement(15594..16712)
                     /locus_tag="JN331_001448"
     CDS             complement(15594..16712)
                     /locus_tag="JN331_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY7259630.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAVGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(16709..17176)
                     /locus_tag="JN331_001449"
     CDS             complement(16709..17176)
                     /locus_tag="JN331_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY7259631.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            17362..17490
                     /gene="mntS"
                     /locus_tag="JN331_001450"
     CDS             17362..17490
                     /gene="mntS"
                     /locus_tag="JN331_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY7259632.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            17762..19348
                     /locus_tag="JN331_001451"
     CDS             17762..19348
                     /locus_tag="JN331_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7259633.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETSFVQSKETSCYL
                     YTMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQV
                     PFMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITN
                     FQLQKVDYNHLGTDIFDPKP"
     gene            complement(19396..19911)
                     /gene="ompX"
                     /locus_tag="JN331_001452"
     CDS             complement(19396..19911)
                     /gene="ompX"
                     /locus_tag="JN331_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY7259634.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(19964..20029)
                     /locus_tag="JN331_001453"
     CDS             complement(19964..20029)
                     /locus_tag="JN331_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259635.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            20264..21151
                     /gene="rhtA"
                     /locus_tag="JN331_001454"
     CDS             20264..21151
                     /gene="rhtA"
                     /locus_tag="JN331_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY7259636.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            21450..21953
                     /gene="dps"
                     /locus_tag="JN331_001455"
                     /gene_synonym="pexB"
     CDS             21450..21953
                     /gene="dps"
                     /locus_tag="JN331_001455"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY7259637.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            22357..23103
                     /gene="glnH"
                     /locus_tag="JN331_001456"
     CDS             22357..23103
                     /gene="glnH"
                     /locus_tag="JN331_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY7259638.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            23242..23901
                     /gene="glnP"
                     /locus_tag="JN331_001457"
     CDS             23242..23901
                     /gene="glnP"
                     /locus_tag="JN331_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY7259639.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            23898..24620
                     /gene="glnQ"
                     /locus_tag="JN331_001458"
     CDS             23898..24620
                     /gene="glnQ"
                     /locus_tag="JN331_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY7259640.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVIQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            24881..27106
                     /gene="ybiO"
                     /locus_tag="JN331_001459"
     CDS             24881..27106
                     /gene="ybiO"
                     /locus_tag="JN331_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY7259641.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRVPVQTYQVLPAPGAPPAEPLPPGEPT
                     L"
     gene            complement(27103..28029)
                     /gene="rlmF"
                     /locus_tag="JN331_001460"
     CDS             complement(27103..28029)
                     /gene="rlmF"
                     /locus_tag="JN331_001460"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY7259642.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            28305..28565
                     /locus_tag="JN331_001461"
     CDS             28305..28565
                     /locus_tag="JN331_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7259643.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            28830..>29129
                     /locus_tag="JN331_001462"
     CDS             28830..>29129
                     /locus_tag="JN331_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY7259644.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         7018 a         7767 c         7305 g         7039 t
ORIGIN      
        1 ttatcgaacg gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg
       61 gccatcaact cgatattttt gtttttctcc agcagtgtgg cctgctcgta gggaatgggg
      121 aaagcaaact gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc
      181 ggacgccagg ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt
      241 ttcaccttca caaaatcggt ctgattccag gtatccagtt catactggtc cggttcccac
      301 cggatgaaaa ccaatctcct tgccatattt ttccagcgct gtcggtgaaa tcatcgcggt
      361 cgccggatgg gcaagaatat taataaacgc tgagaacggc tgtttgaggg taatctttac
      421 cgttgtcgga tcgatcgcct ccgttttggc aatattctta tacaggttat agcgtttaag
      481 atgattcgcc ggatcgctgg cccggtccag attcgctttt accgccactg cgttgaaatc
      541 ggtgccatcc tggaatttaa ttccttcccg caatttcacg gtgtaagtaa tgccgtcatc
      601 ggaaacggta taactctccg ccagcacgtt tttcagtttc atctctttat ccagaccgaa
      661 cagcccctag taaaacgatt tcgctacggc ctgagataac gtgtcattcg cgtcatacgg
      721 atcgagcgtg gtgaaattcg atcctaccgc caccaccaca tctttggcag cgaatgcaca
      781 agatgccatc aacgctgtcg caatgcccag cgccactaac ccactacggt gtacagctct
      841 tgccatttta ttctccgcct gaatgttatc tacgcatgaa tgcgtattct gattgtggtt
      901 gtgcgacgta atgccccggc ccgacgcatt gcaacgagac ggctgccacc tcttcgccac
      961 gcagatgaat attgctggga agatcgtccg acagcagtac acgctgcggt cgttgtcggg
     1021 acggttcagc gaccggaact gccgccagta atttacgcgt ataaggatgc tgcgggtttt
     1081 cgaagacctc gcaccgtgaa ccaatttcaa caatttgccc gagatacatc accgccacac
     1141 gatgactaat ccgctctacc acagccatat cgtgggagat aaacagatac gcaatgccga
     1201 aatcacgctg gagatcgagc aacaagttga taatctgccc gcgaatagaa acatccagcg
     1261 ccgaaacggc ttcgtcggca atgatcactt ttggattcaa tgccaacgcg cgagcaatgc
     1321 agatgcgctg gcgctgaccg ccggaaaact catgcgggta acgccaggca tattcaggta
     1381 acaggcccac gcgctccagc aaccacgcaa cgcgtgcagc cgcgtcttta cctggcaata
     1441 aaccgtgtac acgcagcggt tcgataatcg aatcaccgat ggtctgacgt gggtccagcg
     1501 aagcgtaagg gtcctgaaaa ataaactgaa tatcccggcg caatgcctga agtttgccag
     1561 gtgacaaggt atcgattcgc tgaccgttaa agataatttc gccgccctgc gattcgacca
     1621 ggcgcagcaa cgcccgcccg gtagtggatt taccgctgcc agactcgccc accagcgata
     1681 gcgtttcgcc aggccagaga tcaaaactga ctttctcaac ggcatgcact tcccgcgtta
     1741 cgcgattcaa caaaccgctg cgcaaaggga aacgggtgac aagattacgc acccgtaaaa
     1801 caggttcgcc atccaccacc gttttctgct cgatgggggg gtcctgtttc gctggatgtt
     1861 caagcgatat caatgggaaa cgtcggggat aatctaaccc tttcatcgca ccaagttgcg
     1921 gaacagcagc taacagcgca cgggtgtaag gatgttgcgg tgcatgaaaa atctgttcga
     1981 cggtacccgt ttccaccgcc tcgccctgat acatcaccag tacccgatcg gcaatctctg
     2041 ccaccacgcc catatcgtga gtgataaaga taacgcccat cgacatctct ttttgcaata
     2101 ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc cagcgcggtg gttggctcat
     2161 cggcaatcag caccgccggg cggcatgaca gcgccatcgc aatcatcact cgctggcgca
     2221 tcccgccaga gagttgatgc gaataacgtg aaagaatggt ttgtgcctcc ggaatgcgta
     2281 cctgatccag catccgcttc gcctcgacca tcgcttcttc acgactggcg ttctgatgca
     2341 gacgaattga ttcggcaatc tgttcaccca cagtaaatac tgggttcagc gatgtcatcg
     2401 gctcctgaaa tatcatcgcc atatccgcac cgcgcacatg gcgcatttgt gcagcgctct
     2461 gctcgctaag ttcaatcaca tcacgactgc gccgccgcaa cagcatttta tcgcactgta
     2521 ctaaaccgcc cgcctgttcc aacagacgca tcaatgccag cgcagtcact gacttaccgg
     2581 agccggattc gcctacaatt gccagcgtct caccgcgttg cagactaaaa gagagattgc
     2641 ggaccgcagc tattttctgc tggtcctgca taaaggcaat attaagattt tcaaccgcca
     2701 gcacattacc ggcatcaagt tcatcactgt gtggcaacgg tgtccccttt ttcacggtag
     2761 ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc gatacattcc ttcggtgtta
     2821 aacggtagcg cgacattccc ttcatggtcg atagcgatta agccaccgct accgccaagc
     2881 gcagggagtt tttccattac tacccgctcg caggcttccg cgagacttaa tccgccgtaa
     2941 tccattaacg cggcgatgtc atatgccgcc agcgcgcgga tgaagacttc gcctgtgccg
     3001 gtacaagaaa ccgccacact ggcgttattg gcgtagcatc cggcacccac taagggacta
     3061 tcgccaactc gtccgggtaa tttattggtc attccgcctg tggacgtggc tgccgccaga
     3121 ttgccgtcta aatccaacgc cacggccccc acggtgccca ttttctgttt ttcatccagt
     3181 ggcgcaccgc tatggtcgag gactgttgcc ccttcctcgc gcgctgccag tagttgttca
     3241 taacgcaaag gcgtggagaa aatctccggt gagacgcgct ccatgccatg agcaaacgca
     3301 aaattttctg ccccttcgcc aatcatcatc acatgcgggc tttgctccat caccagccgg
     3361 gcggcaagaa ccggattacg cagatgacta acgcccgcca ccgcaccggc tttcagggtg
     3421 ttaccatcca tcacacaggc gtccagttca tgggtttcat cacgcgtaaa gacagcgcca
     3481 attccggcgt taaacagtgg acactcttcc agcagacgca ccgcttccgt caccacatcc
     3541 agcgcacttt cgccggcttc cagcattttc tgcccggttt caacaatggc agacaacgcc
     3601 tcgatgtagc gtaattcctg ttgcagactc atctgcgcgc ggctaattgc acctgcgcca
     3661 ccatgaattg caatgactgc tttgcccata gtattcgtcc attaagggtg gctcgccact
     3721 tttgctataa atatcagaat ttttatgaat tactcattca atttttgaat atagaaagat
     3781 ggatatggca tgtaaaggca ggagccaacg ctacgcccga ggtggcatac agtctatggc
     3841 gtttttctga cataataggc aaattcgatt ttgcctccgc aggagtgttt tcatggaatt
     3901 taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag atgctttctc gcgttacccc
     3961 actgaccacc caggaaacgc tgccactggt tcagtgtttt ggtcgtattc tggcgagcga
     4021 tgtcgtttcg ccactggatg tcccggggtt tgataactcc gctatggacg gctacgcggt
     4081 gcgtttagcc gatattgcct ccgggcaacc gctgcccgtt gccggtaaat cctttgccgg
     4141 tcagccatac catggtgaat ggcctgcggg tacctgcatt cgtattatga ccggtgcgcc
     4201 ggtgccggaa ggctgcgaag cggtggtgat gcaggagcag actgaacaaa cggacaatgg
     4261 cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt cgccgtcgcg gtgaagatat
     4321 ctctgcaggt gcggttgttt tcccggcggg aactcgcctg actaccgcag agctgccagt
     4381 aattgcttca ctggggattg ccgaagttcc ggtgattcgt aaagtgcgtg tagcgctttt
     4441 ttctaccggt gatgaactcc agttgcccgg tcagccgctg ggcgacggcc aaatctacga
     4501 caccaaccgt ctcgccgtac acctgatgtt agaacagctg ggttgcgagg taatcaactt
     4561 agggattatc cgcgacgatc cacatgccct gcgcgccgca tttattgaag ccgacagcca
     4621 ggcggatgta gtgatcagtt ccggcggtgt ttcagtgggt gaagcggatt acaccaaaac
     4681 gattcttgaa gagctggggg agatcgcctt ctggaagctg gcgattaaac caggtaaacc
     4741 gttcgcgttc ggtaaactca gcaatagctg gttctgcggc ctgccgggca acccggtttc
     4801 agcgacgctg accttctatc aactggtaca gcctttgctg gcaaaactaa gcggcaacac
     4861 cgccagcggc ctgcccgcgc gccagcgcgt acgcacagcg tcccgcctga aaaaaacgcc
     4921 aggacgtctt gatttccagc gcggcgtgct gcaacgcaac gccgacggtg aacttgaagt
     4981 gacgaccacc ggacatcagg gttcacatat atttagctcc tttagtctcg gcaactgctt
     5041 tatcgtgctg gaacgcgatc gcggcaatgt ggacgtgggc gaatgggtgg aagtagaacc
     5101 gtttaacgcg ttgttcggag gcctgtaatg gcggaactca gcgatcagga gatgctgcgc
     5161 tacaaccggc aaatcattct gcgcggtttt gattttgacg gtcaggaggc gctaaaagat
     5221 tctcgtgtgc tggtagtcgg cctcggtgga ctcggctgcg cagcttcgca gtatctggca
     5281 agcgccggtg tcggcaacct gacgctgctc gacttcgaca cggtttcgct ctcgaatctg
     5341 caacgccaga cactgcacag tgatgccacg gtcgggcaac cgaaggtgga atccgcccgt
     5401 gacgccctga cgcggatcaa cccacatatc gcgattacgc cagtcaatgc actgctagat
     5461 gacgcagaac ttgcagcatt gattgctgaa cacgatctgg tgctcgactg tacggataac
     5521 gttgcggtac gtaatcaact gaacgcaggc tgttttgccg cgaaggtgcc gctggtttcc
     5581 ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca cttatcagga cggcgaaccg
     5641 tgctatcgct gccttagccg tttgtttggc gagaacgcgt taacctgcgt agaagcaggc
     5701 gtgatggcac cgttgatcgg cgtaattggt tcgttgcagg caatggaagc gatcaaactg
     5761 ctggcaggtt atggcaaacc ggccagcggg aaaatcgtca tgtacgatgc gatgacctgt
     5821 cagtttcgcg aaatgaaact gatgcgcaat ccggggtgtg aggtgtgcgg gcagtaattg
     5881 ccacctccag gcctgataag acgcgacaag cgtcgcatca ggcatttgct ccagacgcca
     5941 gatgcggcgt gaacgcttta tctggcctac ggttcgggca cagatttcca agcgcggcag
     6001 gcatcggtgc actccgccac gtcatcagca atttatgagg atgcagaacg tccggttaaa
     6061 tcgacgttct gccaaacgct ccctgccagt cctgctcaaa cttcgccaca gcggcttcca
     6121 ccgccggata gctaatcatc tgttgtgcca catccagtgg cagagtaatt gattcacatc
     6181 ctgccagtaa gcagtccagc gcctgacgcg gggttttgaa actcgctgcc agcactttcg
     6241 cctgcggcgc atgcattttc aataactggt gtaagtcggt cacagtctga atgccgctac
     6301 cgccctgagc atcaatacga ttaacgtaag gcgcaacata ttccgcacct gccagcgccg
     6361 acagcagccc ttgtgccgcg ccatataccg cggttcccag cgtcggaatc ccttccgctt
     6421 ttaacatctt aatcgctgcc agcccctcgg ctgtcaccgg aactttcacc acgatatccg
     6481 caataataga acgcagctta agcgcgtcat tcaccatccc ttcagcagtg gtagccatta
     6541 cctgggcaaa cagacgcccc tgaccgccca tcgcttcatg aagttgcgga agcacaacat
     6601 ccagcggttt tttacccgcg gcgataatgc ttgggttagt ggtcacaccc gccagcggaa
     6661 aaatacgtga cagcgccttc accgcaacaa cgtctgaagt atccagatac agttccataa
     6721 ccatcctcaa aatgtacttt ttgtctgaat gtagactatc acgggaaaac tctctgaaca
     6781 gttgacgcac atcaatctaa ctttcattcg aaagtatttt aatgtttgaa cgaaacagag
     6841 aggcaactat gattttcaat attcagcgct actcgaccca tgatggcccc ggtatccgca
     6901 cggtcgtatt tcttaaaggc tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc
     6961 gcgcccgtac gcaggatctg ctgtatgacg cacgactgtg tctggaaggc tgcgagctgt
     7021 gcgctaaggc cgcgccggaa gtgattgagc gcgcgctgaa tggtttgctt attcatcggg
     7081 aaaagttaac cccggagcat ctgacggcgt taaccgactg ctgtccgaca caggcgttaa
     7141 ccgtgtgtgg tgaagtgaaa agcgttgagg agatcatggc gaccgttctg cgcgataaac
     7201 cgttttacga tcgcagcggc ggcggcttaa cgctttcggg tggtgaaccc ttcatgcagc
     7261 cggaaatggc gacggcgctg ctgcaagcct gccacgaggc aggcattcat actgcggtag
     7321 aaacctgtct gcatgtgccg tggaaatata tcgccccttc tctgccctat atcgatctgt
     7381 ttcttgccga tttaaaacac gttgccgacg cgccgtttaa acagtggacc gacggtaacg
     7441 ccgccagggt gctggataac ctgaaaaaac tccccgcagc gggcaaaaaa atcattatcc
     7501 gcgtgccgct gattcagggc tttaatgccg acgaaacctc tgtaaaagcc attaccgatt
     7561 ttgccgccga cgagctgcac gttggcgaaa ttcattttct gccctaccac acgctgggca
     7621 tcaacaaata tcacttactt aatctgccct atgacgcccc ggaaaaaccg cttgatgcgc
     7681 cagagctgct cgattttgcc cagcagtatg cctgccagaa agggttaacc gcgaccttac
     7741 gaggataaca accatgacca cactgaaact ggacacgctc agcgaccgca ttaaagcgca
     7801 caaaaatgcg ctggtgcata ttgtgaaacc gccagtctgt accgagcgcg cgcagcacta
     7861 taccgagatg tatcaacaac atctcgataa gccgatcccg gtacgtcgcg cgctggcact
     7921 ggcgcatcac ctggcgaatc gcaccatctg gatcaaacac gatgagttga tcattggcaa
     7981 ccaggcaagc gaagttcgcg ccgcgccgat cttcccggaa tatactgtct cgtggatcga
     8041 aaaagagatt gatgatctgg cagatcgtcc cggtgctggc tttgcggtga gcgaagagaa
     8101 caaacgtgtt ctgcatgaag tgtgcccgtg gtggcgcggt cagacggtac aggatcgctg
     8161 ctatggcatg tttaccgatg agcaaaaagg tctgctggcg accggcatca ttaaagcgga
     8221 aggcaatatg acctccggcg atgcgcacct ggcggtcaat ttcccgctgc tgctggaaaa
     8281 agggcttgat ggtctgcgtg agaaagtagc ggaacgtcgc tcgcgcatca atctgacggt
     8341 gctggaagat ttgcacggcg aacagttcct gaaagcgatt gatatcgtgc tggtggcagt
     8401 cagtgaacac attgaacgtt tcgctgccct ggcgcgtgca atggccgcga ccgaaacccg
     8461 cgaaagccgt cgcgatgaac tgctggcgat ggcagaaaac tgcgatctta tcgcccacca
     8521 gccgccgcag actttctggc aggcgctgca actgtgttac ttcatccagt tgattttgca
     8581 gattgaatct aacggtcact cggtgtcgtt tggtcgtatg gatcagtatc tctacccgta
     8641 ctatcgccgc gacgttgaac tgaaccagac gctggatcgc gaacacgcca tcgaattgct
     8701 gcatagctgc tggttgaaat tgctggaagt gaacaaaatc cgctccggct cccactcaaa
     8761 agcctctgcg ggaagtccgc tgtatcagaa cgtcaccatt ggcggacaaa atctggttga
     8821 tggtcaacca atggacgcgg tgaatccact ctcttacgcg atcctcgaat cctgcggtcg
     8881 cctgcgttcc actcagccta acctcagcgt gcgttaccat gcaggaatga gtaacgattt
     8941 cctcgacgcc tgcgtacagg tgatccgctg cggcttcggg atgccggcat tcaacaacga
     9001 cgaaatcgtg atcccggaat ttattaaact cggtattgaa ccgcaggacg cttatgacta
     9061 cgcagcgatt ggttgtatag aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg
     9121 catgagcttt atcaacttcg cccgcgtgat gctggcggcg ctggaaggcg ggcgtgatgc
     9181 caccagcggc aaagtgttcc tgccacaaga aaaagcgttg tcggcaggta acttcaacaa
     9241 cttcgatgaa gtgatggacg cgtgggatac gcaaatccgt tactacacgc gtaaatcaat
     9301 cgaaatcgaa tatgtcgtcg acaccatgct ggaagagaac gtgcacgata ttctctgctc
     9361 ggcgctggtg gatgactgta ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata
     9421 tgactgggtt tctggcctgc aggtcggtat tgccaacctc ggcaacagcc tggcggcagt
     9481 gaagaaactg gtgtttgaac agggcgcgat tggtcagcaa cagctggctg ccgcactggc
     9541 ggatgacttc gatggcctga ctcatgagca actgcgtcag cggctgatta acggtgcgcc
     9601 gaagtacggc aacgacgatg ataccgtcga tacgctgctg gctcgcgctt atcagaccta
     9661 tatcgacgaa ctgaaacagt accataaccc acgctacggt cgtggtccgg ttggcggcaa
     9721 ctattacgcg ggtacgtcgt ctatctccgc taacgtaccg tttggcgcgc aaactatggc
     9781 aacgccggac gggcgtaaag cccacacccc gctggcagaa ggcgctagtc cggcctctgg
     9841 tactgaccat ctcggcccta ctgcggtcat tggctcagtg ggtaaactgc ctacggcagc
     9901 gattctcggc ggcgtgttgc tcaaccagaa actgaatccg gcaacgctgg agaacgaatc
     9961 tgacaagcag aaactgatga tcctgctgcg taccttcttt gaagtgcata aaggctggca
    10021 tattcagtac aacatcgttt cccgcgaaac gctgctggag gcgaaaaaac atccggatca
    10081 gtaccgcgat ctggtagtac gtgtcgcggg ttattccgcc ttcttcaccg cgctctctcc
    10141 agacgctcag gacgatatca tcgcccgtac tgaacatatg ctgtaatctc ttcggcccgg
    10201 ctgcggcgtc gccgggctaa atcattcaca tcatcaattt catccttact ttcattcgaa
    10261 atataatttg tgctctgcgt cacattgtta ttacactgtt acggtttcat tttcgagcca
    10321 ggagcacacc tatgagcgta aaagttatcg tcacagatat ggacggtact tttcttaacg
    10381 acgccaaaac gtacaaccaa ccacgtttta tggcgcaata tcaggaactg aaaaagcgcg
    10441 gcattaagtt cgttgtcgcc agcggtaatc agtattacca gcttatttcg ttctttcctg
    10501 agctaaagga tgagatctct tttgtcgcgg aaaacggcgc actggtttac gaacatggca
    10561 agcaactgtt ccacggcgaa ctgacccgac atgaatcgcg gattgttatt ggcgagttgc
    10621 taaaagataa gcaactcaat tttgtcgcct gcggtctgca aagtgcatat gtcagcgaaa
    10681 atgcccccga agcatttgtc gcactgatgg caaaacacta ccatcgcctg aaacctgtaa
    10741 aagattatca ggagattgac gacgtactgt tcaagttttc gctcaacctg ccggatgaac
    10801 aaatcccgtt agtgatcgac aaactacaca tagcgctgga tggcattatg aaacccgtca
    10861 ccagtggttt tggctttatc gacctgatta ttcccggtct acataaagca aacggtattt
    10921 cgcggttact gaaacgctgg gatctgtcac cgcaaaatgt ggtagcgatt ggcgacagcg
    10981 gtaacgatgc ggagatgctg aaaatggcgc gttattcctt tgcgatgggc aatgctgcgg
    11041 aaaacattaa acaaatcgcc cgttacgcta ccgatgataa taatcatgaa ggcgcgctga
    11101 atgtgattca ggctgtgctg gataacacat ccccttttaa cagctgacct ctctaccgga
    11161 gccatgcgct ccggttttcc cttcttttta catcctgtac atcacatcaa atcattaaac
    11221 ttgcattaac ttatttataa cttgtattgt cacatgcaaa tcagcatagt ttcgaatgaa
    11281 agttaatgcg aggtggttat ggcttctact tttaccagcg acacattgcc tgccgatcac
    11341 aaagcagcta tccgtcagat gaagcacgcg ctgcgggcgc agcttggcga tgtccagcag
    11401 atctttaatc agctaagcga tgacattgcc acgcgagtgg ctgaaatcaa cgcactcaaa
    11461 gcacagggcg atgccgtctg gccggtgctg tcttatgccg atatcaaagc aggtcatgtt
    11521 actgcagagc agcgcgaaca gattaaacgt cgcggttgtg cggtgataaa aggccatttc
    11581 ccccgcgaac aagcgctagg ctgggatcag tcgatgctgg actatctgga ccgcaaccgc
    11641 tttgacgagg tctacaaagg ccccggcgat aatttcttcg ggacgctcag cgcttcacgt
    11701 cccgagattt accccatcta ctggtcgcag gcgcaaatgc aggcccgcca gagtgaagaa
    11761 atggcgaatg cgcagtcgtt tctcaatcgt ctgtggacat ttgaaagtga tggaaagcaa
    11821 tggtttaacc cggatgtgag cgtcatctac cctaaccgta tccgccgcct tccgcccgga
    11881 acgacctcca aaggtcttgg agcgcatacc gactccgggg cgctggaacg ctggctgctt
    11941 ccagcatatc agcacgtttt cgccaacgtc tttaatggca atctggcgaa atacgatccc
    12001 tggcatgcgg cacatcgtac ggaagttgaa gagtacacgg tggacaacac taccaaatgt
    12061 tccgtgtttc ggacattcca gggctggact gcgctctcgg atatgctgcc tggtcagggg
    12121 ttgctgcacg tcgtgcccat tcctgaagcc atggcgtacg tactgttacg tccgctgctt
    12181 gatgatgtgc cggaggatga actgtgcggc gtagcgcccg gaagagtgtt gccggtatca
    12241 gagcaatggc atccactgtt aattgaggcg ttaaccagca ttccaaaact cgaggccgga
    12301 gactccgtct ggtggcactg cgacgtcatc cattccgttg cccccgttga aaatcaacag
    12361 ggttggggca acgtgatgta cattcctgcg gcaccgatgt gcgagaaaaa tcttgcctac
    12421 gcgcacaagg tgaaggccgc actggaaaaa ggcgcatcgc cgggcgactt cccgcgcgag
    12481 gactatgaaa caaactggga aggacgcttt acgcttgccg acctcaacat tcacggtaag
    12541 cgagcgttgg gcatggatgt ttgagagtta atggaagtga attgatgtgg gattaagaaa
    12601 atcgattcga cgttactcag attgctgaca agtgcgcttt cttaatgcct gatgcggcgt
    12661 taacgcctta tccggcctac aaagtcctgc agattcaata tattacaggt actgcgtagg
    12721 cctgataagc gtagcgcatc aggctatttt gcatttgtca tcagccgtcg tcaccccgac
    12781 agtatcgggg tgacggataa tcacttactc gatcccttta ctacgcaggt aatcttcgta
    12841 attaccgcta aagtcgatca cgcgttccgg ggtgatttcc agaatgcggg tcgccaggga
    12901 gcttacgaac tcacggtcgt gtgaaacaaa gatcagcgtg ccctgataca gttccagcgc
    12961 catgttcagt gactcgatgg attccatatc caggtggttg gtcggttcgt ccatgatcag
    13021 aatgttcggc ttctgcatca ttaacttacc aaacagcatc cgcccttttt ccccaccgga
    13081 aagcacttta gctggctttt tgatgtcgtc ctggctgaac agcaaacgac cgagaatgct
    13141 gcgtaccgcc tgctcgtcat cgccttcctg cttccactga ctcatccatt cgaacacggt
    13201 cagatcattt tcaaactcat attcgtggtc ctgggcgtag taaccaatgc gcgcgttttc
    13261 agaccatttt acggtgccgc tgtccggttg cagatcgccc accagcgttt tcagcagcgt
    13321 tgatttaccg acgccgttgg tacccagtac cgcaagtttt tcaccgacct ccagcagcag
    13381 gttgagattt ttaaacagtg gaccgttatc aaaccctttg gtcagacctt ccacttccag
    13441 cgcgttacgg aacagtttct tatcctgttc aaaacggatg aacgggttct gacggctgga
    13501 ggctttcacc tcttccagtt tgattttatc aatctggcgc gcgcgcgaag ttgcctggcg
    13561 agatttcgag gcgttggcgc taaagcggct aacgaaagat tgcaactcag caatctgcgc
    13621 tttcttcttg gcgttatcgg ccagcagacg ttcacgcgcc tgggtcgccg ccgtcatgta
    13681 ctcatcgtag ttacccggat aaacgcgcag ctcgccgtaa tccagatccg ccatgtgggt
    13741 acagaccatg ttaaggaagt gacggtcgtg cgagatgatg atcatggtgc tgtcacgctc
    13801 gttcagcacc tgttccagcc agcgaatggt gtcgatgtcg aggttgttgg tcggttcgtc
    13861 gagcaggaga atatccggct cagcaaacag cgcctgcgcc agaagcacac gcagcttcca
    13921 gccaggagca acttcactca tcgggccgta gtgctgttcc actggaattc ccacgccaag
    13981 caacagttca ccggcgcgag cttccgcaga gtaaccgtcc atttcgccgt atttcacttc
    14041 cagatcggcc actttatagc cgtcttcttc actcatttcc ggcaaagcat agatgcggtc
    14101 gcgctcctgc ttcacttccc acaactcttt atgccccatg atcaccgtat ccagcacagt
    14161 gaactcttca aaggcaaact gatcctgacg cagcttacca atgcgctcgt tgggatcgag
    14221 ggaaacgtta cccagcgtcg gctcaaggtc gccgccgaga atcttcataa aggtggattt
    14281 accactaccg ttcgcgccaa tcaggccgta acggttgccg ccgccgaatt tgacggaaat
    14341 gttttcaaat aacggcttac tgccgaactg catggtgacg ttactggaaa ctaacacagg
    14401 cgtatcctga aaagagatat gacaaaccgc gcattatgcc ataagtctga aagaagatcc
    14461 tgctcctcgc ttaaggtcta cactcaagcc aaaggcaaaa aatgtgattt cgtacacatc
    14521 tgatttcact gtgagctgga atgaacttat aatgcgcttc caatactcta atattctcaa
    14581 cccaatggcc tgccaggcac aaaatctcgc ttaacatgaa tatgaaattg aaaacattat
    14641 tcgcagcggc cttcgctgtt gtcggctttt gcagtaccgc ctctgcggta acttatcctc
    14701 tgccaaccga cgggagtcgc ctggttggtc agaatcaggt gatcaccatt cctgaaggta
    14761 acactcagcc gctggagtat tttgccgcgg agtaccagat ggggctttcc aatatgatgg
    14821 aagcgaaccc gggtgtggat accttcctgc cgaaaggcgg tactgtactg aacattccgc
    14881 agcagctgat cctgccggat accgttcatg aaggcatcgt cattaacagt gcagagatgc
    14941 gtctgtatta ctatccgaaa gggaccaaca ccgttatcgt gctgccgatc ggcattggtc
    15001 agttaggcaa agatacgcct atcaactgga ccaccaaagt tgagcataag aaagcaggcc
    15061 cgacctggac gccgaccgcc aaaatgcacg cagagtaccg cgctgcgggc gaaccgctac
    15121 cggctgtcgt tccggcaggt ccggataacc cgatgggtct gtatgcactc tacatcggtc
    15181 gcctgtatgc tatccatggc accaacgcca acttcggtat cggcctgcgt gtaagtcatg
    15241 gttgtgtgcg tctgcgtaac gaagacatca aattcctgtt cgagaaagta ccggtcggta
    15301 cccgcgtaca gtttattgat gagccggtaa aagcgaccac cgagccagac ggcagccgtt
    15361 atattgaagt ccataacccg ctgtctacca ccgaagccca gtttgaaggt caggaaatcg
    15421 tgccaattac cctgacgaag agcgtgcaga cagtgaccgg tcagccagat gttgacctgg
    15481 ttgttcttga tgaagcgatc aaaaatcgtt ccgggatgcc ggttcgcctg aattaatcgt
    15541 caaaacgtaa aacaaaggcg gactcataat ccgccttttt tatttgccag accttaccag
    15601 gccgggatca taactaacaa aacgtatccc accagcgccg cccacaacag catcggtatt
    15661 gaatagagat ggaaacgcca ccagatgcgg cgatcgttcg ccatacgtag cgcaatcaaa
    15721 tttgccagcg atcctggtaa taaaccaaag ccccccacgt ttaccgccca tgccagtaat
    15781 aaagacggag gtacataatt gagcagcaaa atggtactcg gcacgttact gatcacctgc
    15841 gataaaccga ctgccgttaa ccataacccg ggttcagata aatgactcac gttacccaac
    15901 acgccttgca acgctggaag ctgggtcagt aaatggacgt cgataaacat cgccataaac
    15961 accagcagca acgtccagtc cacactcaac accacgcgac gcgccagcag cacaaagccc
    16021 accgccacaa tcaccagtcc ccacagctct tgtttgaact ccagcgccgt cagaaagacg
    16081 atatacagcc ccagacaatt ccacaccagc cgcggtttcc actccggtgt ttgcaccccc
    16141 gtatggtatt gcagcgcctt tccagggaaa caacaccagc acaacagcag gagtgtcagc
    16201 atcattgcgc cagccagcgg tgccatttgg gcaataaatc cggcaaacga aagaccagaa
    16261 cgtccccaga taagaatatt ttgcgggttg ccaattggcg tcagtagcga accagcgttg
    16321 actgccagcg cctcaaaaat aatcagccga ttaaccggga tctcacacag tctttttagc
    16381 gtgatagtca gcggaacaac aataaacagc gcgacatcgt tggtcagaaa ggtagaaagc
    16441 agcgccgccg ccagcaccat aaacatcgcc agccgacgct ccgtagcaaa gcggcgcacc
    16501 attttgcgcc ccagcacatc aaaataaccg cttaactcca cacctttggt cagcagcatc
    16561 aggccgctta aggtgatgat ggtgtgccag tcgatagcag caggccagga tttcggtgca
    16621 aagggcacga aaaagcttaa tccgatacca acaagaatta ataactgaaa aaaacgatcg
    16681 ccttgcagcg tgcgtaaaaa aggcaggctc atttggcacc gtgtttctgg gtaaacaaac
    16741 gaaaagcgtc gagcgtctct tcactaacat ggtgctccat gccttccgcg tcgcgacggg
    16801 cgatttccgg actgacgccc aacaccaaca agaaattttc gactatctga tgtcgctcgc
    16861 ggctttcctg cgccagcttc tctccttctg ccgttaaaaa cacgccacgc caggggatca
    16921 tttcaatcag ccccatggtt gccagccgct taagcatttt agccaccgtc ggttgcgaaa
    16981 ctcccagacg agcagccatg tccacctgac gagcttcccc cacttccctg atcaagtcag
    17041 aaatcagctc aacgtaatca tcaataagct cgcgccgatg cgcctctctg acctggcgga
    17101 acccttcaac gtgctcttcc acgttcacta actgcgtcac tttttttgct gttggcgtac
    17161 ctgcgcgacg actcattgtg cttcctcatt atggtgacgc atacatagcg ttctcatttc
    17221 gtgcgcacat tgtaaaccag agttgcgaag gtacaaaaaa ttaacgtttt agcaatagct
    17281 atataatata gcctgtgcta tatctgtatg taatgcaatc atccctcaag gatcgacggg
    17341 attagcaagt caggaggtct tatgaatgag ttcaagaggt gtatgcgcgt gtttagtcat
    17401 tctcccttta aagtacggtt aatgctgctc tctatgttgt gcgatatggt caacaacaaa
    17461 ccgcagcaag ataaaccttc agataaatag cggcgtcgcg gtacgccgct tcactcctgc
    17521 ttttatgcag gcataacgcg ttttggtctg aaaaaaccca ctttttgtcg gatttgcaat
    17581 ccccttcgca aaagatttgt tcgtcagtag ttgacctgaa cggcggctcg ctctatcttc
    17641 ttgcagccct gcgtatattg cggctcgcgg atgcggaccc ctttcccctc ttcacgcact
    17701 cttgcaggta ttgacccttg acgccagggt aagcacatgg cgtttgttac gatagtggca
    17761 tatgaattta accctcaaag aatcgcttgt tacccgtagc cgggtattta gcccgtggac
    17821 tgcgttctac tttttacagt cgctattaat taacctcggc ttaggttacc ccttcagttt
    17881 gctctacacc gctgcgttta cggctatttt gcttttgcta tggcgaacat tgcctcgcgt
    17941 acaaaaagtt ctggtcggtg tcagttcgct ggtggcggct tgttatttcc cttttgctca
    18001 ggcctacggc gcgcctaatt tcaatacatt gctggcattg cactccacca atatggaaga
    18061 gtcgaccgaa atcctgacga tttttccgtg gtacagctac ctggtcggct tatttatttt
    18121 tgcgctcggc gtaatagcaa tcaggcgaaa aaaagagaat gaaaaagcgc gctggaatac
    18181 cttcgacagc ctgtgtctgg tattcagtgt ggcgacattt tttgttgctc ccgtgcaaaa
    18241 cctggcctgg ggtggcgtat ttaaactgaa agatactggc tatccggtat ttcgttttgc
    18301 taaggatgtc atcgtcaata ataacgaggt gattgaagag caagaacgga tggcaaaact
    18361 ttccggaatg aaagatactt ggacggtcac tgccgttaag ccgaagtatc agacctatgt
    18421 ggtagtgatc ggtgaaagcg cgcgtcgcga tgccctcggt gcctttggcg gtcactggga
    18481 caataccccg tttgccagca gcgttaacgg tttgatattt gctgactaca ttgccgccag
    18541 tggctccacg cagaaatcgc ttggcttaac gctcaatcgc gtagtcgatg gcaaaccaca
    18601 gtttcaggat aactttgtca ccctggcaaa tcgcgcgggc ttccagacct ggtggttttc
    18661 caaccagggc caaatcggcg aatacgatac tgctatcgcc agcatcgcca aacgtgcaga
    18721 tgaagtgtac ttcctgaaag aaggtaattt tgaagcagat aaaaacacga aagacgaagc
    18781 gttactggat atgaccgctc aagtgctggc gcaagagcac tcgcaaccgc agctgattgt
    18841 tctgcatctg atgggctcgc atccgcaggc ctgcgacagg acacaaggca aatacgaaac
    18901 ctcctttgtg caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga
    18961 tttactgcgc aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta
    19021 cttttctgac cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca
    19081 tgatgataaa tatcagcaaa atttccaggt gccttttatg gtcatttcca gcggcgataa
    19141 agcgcatcgg gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca
    19201 gtggacggga attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa
    19261 agccgggccg atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg
    19321 aaccgatatt ttcgacccga aaccttaaaa acaaaaatcc gccccgagag gcggattttt
    19381 atatcaccaa agtgattaga agcggtaacc aacaccggca atccaggtgc ctacgtcaac
    19441 gctacgaata cggctctgct cgtaagagaa gtccagagca acgttttcca tcgggttgaa
    19501 ctgcagacca gcaccgtagg agaaaccgta gtcgctggtg tcgtgtttgt aggtcgggta
    19561 ttcagtggtc tggaatttac cataacccac acccactaca ccgtagatgc ttgcccagtc
    19621 gttaatgcgg taagccggac cagcagtgat gccatagtac tggtttttgt tgtagtcacc
    19681 agagcttgca gtacggcttt tctcggtgta agtgaaagaa ccgatcacac ccagcgggct
    19741 gttgtcttct tcatagcggt atttcaggtt gaaaccgccc attttgttca tttggccctg
    19801 agcgtcgctc tgtgcgtaac cgccagttac ggtagaagtc gcagctacgg aagtacctgc
    19861 ggtgaaagcc agaactgcgg ccagtgctga aagacatgca atttttttca taaccacctc
    19921 aaatgtgatt caaataagtc ctaagtttta aatatatcaa aaattaatgg gaaactcttc
    19981 gcgatttgtg atgtctaacg ggccatttca tgtaacagaa cgtttccata caccgctatc
    20041 catctaaatt taaatcactt tttcagagaa ctgcgtaagt attacgcatg ttttccctgt
    20101 cattcatcca gattattcct aatcaccaga ctaatgattc catcaatcct ggcgcatttt
    20161 agtcaaaacg ggggaaaatt ttttcaacaa atgctcaacc agcattgggt atatccagta
    20221 cactccacgc tttacttaag tctagatatt tgtgggagaa aggatgcctg gttcattacg
    20281 taaaatgccg gtctggttac caatagtcat attgctcgtt gccatggcgt ctattcaggg
    20341 tggagcctcg ttagctaagt cactttttcc tctggtgggc gcaccgggtg tcactgcgct
    20401 gcgtctggca ttaggaacgc tgatcctcat cgcgttcttt aagccatggc gactgcgctt
    20461 tgccaaagag caacggttac cgctgttgtt ttacggcgtt tcgctgggtg ggatgaatta
    20521 tcttttttat ctttctattc agacagtacc gctgggtatt gcggtggcgc tggagttcac
    20581 cggaccactg gcggtggcgc tgttctcttc tcgtcgcccg gtagatttcg tctgggttgt
    20641 gctggcggtt cttggtctgt ggttcctgct accgctgggg caagacgttt cccatgtcga
    20701 tttaaccggc tgtgcgctgg cactgggggc cggggcttgt tgggctattt acattttaag
    20761 tgggcaacgc gcaggagcgg aacatggccc tgcgacggtg gcaattggtt cgttgattgc
    20821 agcgttaatt ttcgtgccaa ttggagcgct tcaggctggt gaagcactct ggcactggtc
    20881 ggttattcca ttgggtctgg ctgtcgctat tctctcgacc gctctgcctt attcgctgga
    20941 aatgattgcc ctcacccgtt tgccaacacg gacatttggt acgctgatga gcatggaacc
    21001 ggcgctggct gccgtttccg ggatgatttt cctcggagaa acactgacac ccatacagct
    21061 gctggcgctc ggcgctatca tcgccgcttc aatggggtct acgctgacag tacgcaaaga
    21121 gagcaaaata aaagaattag acattaatta aatttacatt tctgtatggt tatgcataac
    21181 catgcagaat ttctcgctac ttttcctcta caccgtcttt atatatcgaa ttatgcaaaa
    21241 gcatatttat tccgaaaatt cctggcgagc agataaataa gaattgttct tatcaatata
    21301 tctaactcat tgaatcttta ttagttttgt ttttcacgct tgttaccact attagtgtga
    21361 taggaacagc cagaatagcg gaacacatag ccggtgctat acttagtctc gttaattact
    21421 gggacataac atcaagagga tatgaaatta tgagtaccgc taaattagtt aaatcaaaag
    21481 cgaccaatct gctttatacc cgcaacgatg tctccgacag cgagaaaaaa gcaacagtag
    21541 agttgctgaa tcgccaagtt atccagttta ttgatctttc tttgattacc aaacaagcgc
    21601 actggaacat gcgcggcgct aacttcattg ccgtacatga aatgctggat ggcttccgca
    21661 ccgcactgat cgatcatctg gataccatgg cagaacgtgc cgtgcagctg ggcggtgtag
    21721 ctctggggac cactcaagtt atcaacagca aaaccccgct gaaaagttac ccgctggaca
    21781 tccacaacgt tcaggatcac ctgaaagaac tggctgaccg ttacgcaatc gtcgctaatg
    21841 acgtacgcaa agcgattggc gaagcgaaag atgacgacac cgcagatatc ctgaccgccg
    21901 cgtctcgcga cctggataaa ttcctgtggt ttatcgagtc taacatcgaa taaatccatc
    21961 gctgatggtg cagaacttta gtacccgata aaagcggctt cctgacagga ggccgttttg
    22021 ttttgcagcc cacctcaacg cacttattta gtgcatccat ctgctatctc cagctgatta
    22081 agtaaatttt ttgtatccac atcatcacac aatcgttaca taaagattgt tttttcatca
    22141 ggttttacgc taaataatca ctgtgttgag tgcacaattt tagcgcacca gattggtgcc
    22201 ccagaatggt gcatcttcag ggtattgccc tataaatcgt gcatcacgtt tttgccgcat
    22261 ctcgaaaaat caatgagttg caaaactggc acgatttttt catatatgtg aatgtcacgc
    22321 aggggatcgt cccgtggata gaaaaaagga aatgctatga agtctgtatt aaaagtttca
    22381 ctggctgcac tgaccctggc ttttgcggtt tcttctcatg ccgcggataa aaaattagtt
    22441 gtcgcgacgg ataccgcctt cgttccgttt gaatttaaac agggcgataa atatgtgggc
    22501 tttgacgttg atctgtgggc tgccatcgct aaagagctga agctggatta cgaactgaag
    22561 ccgatggatt tcagtgggat cattccggca ctgcaaacca aaaacgtcga tctggcgctg
    22621 gcgggcatta ccatcaccga cgagcgtaaa aaagcgatcg atttctctga cggctactac
    22681 aaaagcggcc tgttagtgat ggtgaaagct aacaataacg atgtgaaaag cgtgaaagat
    22741 ctcgacggga aagtggttgc tgtgaagagc ggtactggct ccgttgatta cgcgaaagca
    22801 aacatcaaaa ctaaagatct gcgtcagttc ccgaacatcg ataacgccta tatggaactg
    22861 ggcaccaacc gcgcagacgc cgttctgcac gatacgccaa acattctgta cttcatcaaa
    22921 accgccggta acggtcagtt caaagcggta ggtgactctc tggaagcgca gcaatacggt
    22981 attgcgttcc cgaaaggtag cgacgagctg cgtgacaaag tcaacggcgc gttgaaaacc
    23041 ctgcgcgaga acggaactta caacgaaatc tacaaaaaat ggttcggtac tgaaccgaaa
    23101 taataacgct acacctgtaa agcgcactgg cagttccctc tcccccctgg ggagaggatt
    23161 agggtgaggg gcgcaaaccc gctccggggc cattaattac cctgaatttg attatttaca
    23221 ccacggtaac aggaacaaca tatgcagttt gactggagtg ccatctggcc tgccattccg
    23281 cttctgattg aaggtgccaa aatgaccctg tggatttcgg tcctcggtct ggctggcggt
    23341 ctggtaatcg gattgctggc aggttttgca cgcaccttcg gtggttggat agccaaccac
    23401 gtcgcgctgg tctttattga agtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt
    23461 atctatttcg ccctgccgat ggcgtttaac gacttacgca tcgacccatt tactgcggcg
    23521 gtggtcacca tcatgatcaa ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg
    23581 ctgtctatcc acaaaggttt tcgtgaagca ggactggcgc tcggtctttc acgttgggaa
    23641 accattcgct acgtcatttt accgctggca ctgcgtcgta tgctgccgcc gctgggtaac
    23701 cagtggatca tcagcattaa agacacctcg ctgtttattg ttatcggcgt ggcggaactg
    23761 acccgtcagg ggcaagaaat tattgccggt aacttccgcg cccttgagat ctggagcgcc
    23821 gtggcggtgt tctatctgat tattaccctg gtgctgagct ttattctgcg tcgtctggaa
    23881 agaaggatga aaatcctgtg attgaattta aaaacgtctc caagcacttt ggcccaaccc
    23941 aggtgctgca caatatcgat ttgaacattg cccagggcga agtcgtggtg attatcgggc
    24001 cgtccggttc cggtaaatcg accctgctgc gctgcatcaa caaactggaa gaaatcacct
    24061 ccggcgatct gattgtcgat ggcctgaagg ttaacgatcc gaaagttgac gagcgcctga
    24121 ttcgccagga agcaggtatg gtgttccagc agttttacct cttcccgcat ctgacggcgc
    24181 tggaaaacgt catgtttggt ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa
    24241 aactggcgcg tgagctgctg gcgaaagtcg gtctggcaga acgtgcacat cactaccctt
    24301 ccgaactttc tggtggtcaa cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc
    24361 cgaaaatgat gctgtttgat gaaccgactt ccgctcttga cccggaactg cgccatgaag
    24421 tgctgaaggt tatacaggat ctggctgaag aagggatgac gatggtgatc gtgacccacg
    24481 aaatcggttt tgccgagaaa gtagcttcgc ggctgatctt tatcgacaaa ggccggattg
    24541 cggaagatgg caatccgcag gtgttgatca agaacccgcc gagccagcgc ttgcaggaat
    24601 ttttgcagca cgtctcttaa taagacacat tgcctgatgc gctacgctta tcaggcctac
    24661 aggatatctg gcaacttatt aaaattgcat gaacttgtag gacggataag gcgttcacgc
    24721 cgcatccggc aaaaaagccc gcacgttgtc agcaacctgc ttaatatccc ttcctccctt
    24781 tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc ccccagcatc
    24841 tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga tcctgttcat
    24901 cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg ttacaaccac
    24961 aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac aaaaaaaagc
    25021 ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag agttgatcga
    25081 ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga tcgtgccgcc
    25141 gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc gccattatgg
    25201 tgaagccctt tccgcccgct tcgggcaact ttatcgcaat atcaccggct ccccgcataa
    25261 gccatttaat ccacaaacct tcagcaatgc gctgagccat ttttcaatgt tagcggtatt
    25321 agtgtttggt ttttactggc tgattcgcct gtgcgcactg ccgctgtatc gcaaaatggg
    25381 tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc cggcgatgat
    25441 tattggggcg tttattatcg acctgctgtt actggccctg acattatttg tcggccaagt
    25501 attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa gtttgtttct
    25561 caacgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt tttgcccaaa
    25621 cgtggcggag ctgcgcccgt tcacgattca ggacgagagc gcccgttact ggagtcgtcg
    25681 cctgagctgg ttaagcagcc tgattggcta tggcctgatt gtggccgtgc cgattatctc
    25741 taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc tgtgcatgac
    25801 cgtctgggcg ttgtacctga tcttccgaaa taaaaaagag attacccagc atttgctcaa
    25861 ctttgcggag cattcgctgg cttttttcag cctgtttatc cgcgcctttg cgctggtgtg
    25921 gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt tcgatccggg
    25981 caacagtctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta ttggtatcgc
    26041 agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa accatcaccc tctcgccaca
    26101 tactcagcgt aactatccgg agctacaaaa acggctgaat ggctggcttt cggcggcgct
    26161 gaaaacggcg cgtattctga ccgtctgcgt ggcagtaatg ttgctgttga gcgcatgggg
    26221 attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg tagatatcct
    26281 gatccgcatc gcactcattc ttttcttctc ggcggttggc tggacggtgc tcgccagttt
    26341 gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg cccgtacgcg
    26401 taccctgctg acgctgttcc gtaacgcgct ggcggtgatt atcagtacca tcaccatcat
    26461 gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg ccggggcatt
    26521 aggtctggct atctcgtttg gttcgcaaac gctggtaaaa gatattatca ccggggtatt
    26581 tattcagttt gaaaacggca tgaacactgg agatttggtg actatcgggc cgttgaccgg
    26641 cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc caggataccg gggcgtatca
    26701 catcattccg tggtcttcga taaccacctt tgctaacttc gtccgcggta ttggttcggt
    26761 agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc aggcactgaa
    26821 agatgcggta acggaattga tggaaaacga agaaattcgc gggctgatta ttggtgaacc
    26881 gaactttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg tttcgttcac
    26941 cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg tgaaaaaaca
    27001 tttcgacctg gcgggcgttc gcgtgccagt gcagacttat caggtgctgc ctgctccggg
    27061 cgctcccccg gctgaaccgc tgccgccggg ggagccaacg ctttaacgtt ggcgattaac
    27121 aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac tctgcttttg
    27181 cccctgggcc atctcttttt ttaccacctt caccgcgccc acgtcggtca gagcacgata
    27241 caacggcggt aagttttcac cacgagatac cagcgaggta aaccacatca cctgcttcgc
    27301 gaagcctttg ctctcttcaa tcatcttttt gataaagttg acttcaccgc cttcacacca
    27361 caactcctgt tgctgaccac caaagttcag tgcatcgtct ttgttcagcc ccaggttacg
    27421 gcgtttacgc tcactacctg cccgcgccgc agcggcggaa tcgtggaatg gcgggttaca
    27481 caaggtcgcg tcgtattgct cgtttttgtg gatgatgccg ttaaaaatcg ccccactctc
    27541 tttttgccga cgcagacgaa tggcgcggtt aagccccgga ttagcactga taatcgcctg
    27601 cgcactgctt aacgcctggc tgctggtttc actaccggta aaacgccagc catattcatg
    27661 tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa tgctggcatt
    27721 cgctggaatt gttccgcttg cttcagccag taaatcggca aggtgatgaa tataatccgc
    27781 ccggcccggt actggtgggc agagaaaacc gtcggggata tcccagttcg ctacggcgta
    27841 aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc agcggattgg caaagtctac
    27901 gctttgctcc cctgcgggtg taagcgtgag gaattgcctg agttcaggat tgacctgaca
    27961 aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc ccggtttctg
    28021 ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac gcgacagggt
    28081 aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt ttatggcaat
    28141 tcatcataaa aatcagcatt atgcatgtca ttgatttgtc ttattttgta aaaatatatc
    28201 gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca tttgcatcgc gcaggcgacc
    28261 tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa aagtgcctca
    28321 cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc gcgcaaccga
    28381 tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca actggcgcat
    28441 caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgaacaa ggcgcgaaag
    28501 gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca accatctaca
    28561 aataacccca ccaacgtaac tgcattcgcc tctggatgca gttttcgcct gttttgtaat
    28621 caaaaaacat caaatatgaa cgcaatgtaa ataaatgtat ttctttttcg cgcaatgggt
    28681 gatagaaaat cgcgtcaagt gataatgctt atcaaaatta ttatcacttg tgcgaacact
    28741 atcacgggat taacagtggc atcgcatccg cagagatgct ttctcgtggc agtgaaaatt
    28801 tcaacatata agaaaaagtc acctgcaaaa tggaaaacaa tcgcaatttc cctgccagac
    28861 aatttcattc gctcacgttc tttgccggtc tttgtattgg tattacgcct gtggcacagg
    28921 cactcgccgc cgaagggcaa gctaacgcgg atgacacgct ggttgtcgaa gcatcaacgc
    28981 cttcgcttta tgcgccacaa caatctgccg atccgaaatt ctcgcgtccg gtagcggata
    29041 ctacccgcac gatgacggtg atttctgaac aagtgattaa agatcagggc gcaaccaacc
    29101 ttaccgacgc gctcaaaaac gtccccggc
//
LOCUS       DADLSB010000033        28961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.033, whole genome shotgun sequence.
ACCESSION   DADLSB010000033 DADLSB010000000
VERSION     DADLSB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.033"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..268)
                     /locus_tag="JN331_001463"
     CDS             complement(<1..268)
                     /locus_tag="JN331_001463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259645.1"
                     /translation="MKVQILNNNCEVVWSYDIAAHVDQSGDSWANGKHQIMAGVVFSL
                     RRALEQAEVFPSGPEWKWPFSICPNSESTFQKIGQKVALEEHQPT"
     gene            complement(462..1136)
                     /locus_tag="JN331_001464"
     CDS             complement(462..1136)
                     /locus_tag="JN331_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HAY7259646.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            1227..1427
                     /locus_tag="JN331_001465"
     CDS             1227..1427
                     /locus_tag="JN331_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7259647.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            1471..2022
                     /locus_tag="JN331_001466"
     CDS             1471..2022
                     /locus_tag="JN331_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259648.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            2019..2855
                     /locus_tag="JN331_001467"
     CDS             2019..2855
                     /locus_tag="JN331_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAY7259649.1"
                     /translation="MNSLTANNRLSQQLVVSVAEHLLLRHECRLPNHLAVSNHRELYL
                     TVGGELCRNLTAGFVTEEGFMSMLFVGSQKHSALSIFAKTTRMSALVLCGNSGVILLS
                     VKDHQHIDSAIPGRYTVQAPYKAGAGIGVLEMLSAIYDAPASFLSSASAHTQIMVGWT
                     GALKGAPVSDNAGYANPVQFTTSEIGVSGGEGNSLLSEAAIMATVPALTRLNDEDLHK
                     LSYVTTALRALRKVTLSDPQAHQVLVETLLNLQAERIRLAHKANFHIHRLLNISGGHR
                     HA"
     gene            2860..3084
                     /locus_tag="JN331_001468"
     CDS             2860..3084
                     /locus_tag="JN331_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259650.1"
                     /translation="MILNICRLLQRKKTSIPTVGQWYTTPAGHVLRVSLVDRECQKVV
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            3081..3898
                     /locus_tag="JN331_001469"
                     /pseudo
     CDS             3081..3898
                     /locus_tag="JN331_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3895..4389
                     /locus_tag="JN331_001470"
     CDS             3895..4389
                     /locus_tag="JN331_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY7259651.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQSVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLFRRAATVWMEAFRESHSQPERNNFLAHRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            4389..5042
                     /locus_tag="JN331_001471"
                     /pseudo
     CDS             4389..5042
                     /locus_tag="JN331_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
     gene            5039..5365
                     /locus_tag="JN331_001472"
     CDS             5039..5365
                     /locus_tag="JN331_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAY7259652.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     KRGLKV"
     gene            5362..5751
                     /locus_tag="JN331_001473"
     CDS             5362..5751
                     /locus_tag="JN331_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7259653.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            5771..6580
                     /locus_tag="JN331_001474"
     CDS             5771..6580
                     /locus_tag="JN331_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7259654.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            6624..7577
                     /locus_tag="JN331_001475"
                     /pseudo
     CDS             6624..7577
                     /locus_tag="JN331_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328142.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            7591..8400
                     /locus_tag="JN331_001476"
     CDS             7591..8400
                     /locus_tag="JN331_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7259655.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQLIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARHHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTRQMIFAEDGDMMLAFFKKYG"
     gene            8494..8751
                     /locus_tag="JN331_001477"
     CDS             8494..8751
                     /locus_tag="JN331_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259656.1"
                     /translation="MQGEKQQPYFFNPGMTVEQLEDWLEQQKLHLSRYNRLVKEKAEL
                     EERLSDISVEIERVSAGGFNGKLSFPWESSSLLRNHQQGSV"
     gene            8831..9217
                     /locus_tag="JN331_001478"
     CDS             8831..9217
                     /locus_tag="JN331_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259657.1"
                     /translation="MSDPISGTGLAGGALTGASVYGLLTGTDYGVVFGAFAGAVFYIA
                     TAADLSASRRLAYFIVSYIAGILCSGLVGSKLAHLTGYSDKPLDAIGAVIVSALTVKI
                     LTFLNNQDIGSLVALITRRGGSGGAK"
     gene            9204..9485
                     /locus_tag="JN331_001479"
     CDS             9204..9485
                     /locus_tag="JN331_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAY7259658.1"
                     /translation="MELNDPTATINALLCACVVITLMFYRRGDSRHRPWVSRLAWLIT
                     VTYSAVPLAYLCGIYPHSSWPIIVANTIFLSVLVAVRGNVARLVDHLRH"
     gene            9485..10102
                     /locus_tag="JN331_001480"
     CDS             9485..10102
                     /locus_tag="JN331_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HAY7259659.1"
                     /translation="MNQQLFQKAAGISAGLAARWFPHIDAAMKEFGITAVNDQAMFIA
                     QVGHESAGFTSLVESFNYSVDGLKKTFSKRLTPYQCEMLGRVDGKQVAHQPQIANLVY
                     GDRMGNNSQGDGWKYRGRGLLQITGRENYAKCGAALKLDLISTPELLTQEKHAARSAA
                     WYFTLRGCLMYSGDVVRVTQIINGGQNGLADRNSRYNKARAALLV"
     gene            10099..10629
                     /locus_tag="JN331_001481"
     CDS             10099..10629
                     /locus_tag="JN331_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HAY7259660.1"
                     /translation="MTAVFAFVQARWKTIIVLLMLAGAFLAGNIWSERGWQKKWADHN
                     SMESSQEANAQTAARWIEQGRIIARDEAVKDAQAQAAKSAATAAGLSATVSQLRTEAT
                     KLAARLDAAKHTADLAAAVRSKTTDAGMFADMLGDIAAEAKRYAGIADERYTAGMTCE
                     RIYDSLRESNNAIPHN"
     gene            10696..10971
                     /locus_tag="JN331_001482"
     CDS             10696..10971
                     /locus_tag="JN331_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259661.1"
                     /translation="MSKQGYAVSGHSDGLADILSIVIVLAVEHWIIASLIVGLIMYLV
                     GASINENAQTNAGAIASGIIGVCGQFIFGFGVLASIIGIVKFIWTHT"
     gene            11036..11233
                     /locus_tag="JN331_001483"
     CDS             11036..11233
                     /locus_tag="JN331_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016150493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259662.1"
                     /translation="MTVVLTAKQIEDLAAFAKEDGQPQYTITTGTIPEFEADDGEIIP
                     EYTGLIAYSESLEHGVLQLDD"
     gene            11297..11647
                     /locus_tag="JN331_001484"
     CDS             11297..11647
                     /locus_tag="JN331_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7259663.1"
                     /translation="MPPRTPKACRVRGCRNTTTDPSGYCENHKGEGWKSYKPGQSRQQ
                     RGYGTKWEVIRERILKRDKGLCQNHLRQAIVKQASCVDHIKAKAHGGTDEDSNLESLC
                     WSCHAAKTARERLK"
     gene            11774..12268
                     /locus_tag="JN331_001485"
     CDS             11774..12268
                     /locus_tag="JN331_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7259664.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            12265..13998
                     /locus_tag="JN331_001486"
     CDS             12265..13998
                     /locus_tag="JN331_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7259665.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     SRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDAVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIIQNYTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKTIPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            14010..14192
                     /locus_tag="JN331_001487"
     CDS             14010..14192
                     /locus_tag="JN331_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259666.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPVGFVVAGALCLFWSWL
                     VARYLDRTQQSVGGGK"
     gene            14192..15433
                     /locus_tag="JN331_001488"
     CDS             14192..15433
                     /locus_tag="JN331_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7259667.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLTESVGMLPCNLYHLNGGLKQRSTGERLHKLISSHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLT
                     QEDIWHVRTLTLDGLVGLNPVAYAREAILLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGPDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKLGIYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            15411..16061
                     /locus_tag="JN331_001489"
     CDS             15411..16061
                     /locus_tag="JN331_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY7259668.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSVLNALKNLNF"
     gene            16076..17281
                     /locus_tag="JN331_001490"
     CDS             16076..17281
                     /locus_tag="JN331_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7259669.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIYGNPQATQKMLDDTFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATTVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTCILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            17332..17532
                     /locus_tag="JN331_001491"
     CDS             17332..17532
                     /locus_tag="JN331_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259670.1"
                     /translation="MILKQDLKWSPDGMRVEVIQAGEYDDGALPARVQEIALQAGLAE
                     RGISAKSSKAAKEKKATTSKEG"
     gene            17535..17858
                     /locus_tag="JN331_001492"
     CDS             17535..17858
                     /locus_tag="JN331_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7259671.1"
                     /translation="MLLTMEEIKAQLRLDEDFDTDDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            17855..18265
                     /locus_tag="JN331_001493"
                     /pseudo
     CDS             17855..18265
                     /locus_tag="JN331_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
     gene            18240..18746
                     /locus_tag="JN331_001494"
     CDS             18240..18746
                     /locus_tag="JN331_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259672.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            18743..19303
                     /locus_tag="JN331_001495"
     CDS             18743..19303
                     /locus_tag="JN331_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259673.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            19312..19482
                     /locus_tag="JN331_001496"
     CDS             19312..19482
                     /locus_tag="JN331_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY7259674.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            19466..20962
                     /locus_tag="JN331_001497"
     CDS             19466..20962
                     /locus_tag="JN331_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7259675.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNSAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATV
                     TLTVNGAATETGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPACPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLFHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            20962..21318
                     /locus_tag="JN331_001498"
     CDS             20962..21318
                     /locus_tag="JN331_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7259676.1"
                     /translation="MARIGGTCYFKMDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            21318..21587
                     /locus_tag="JN331_001499"
     CDS             21318..21587
                     /locus_tag="JN331_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAY7259677.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            21729..23564
                     /locus_tag="JN331_001500"
     CDS             21729..23564
                     /locus_tag="JN331_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7259678.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGSPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILILGLMGVGSAISIVSRIMSMTPIGMIATAIAMAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINHWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPVVDLAQSNNASGAIGPNPFMIN
                     KATAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            23625..24953
                     /locus_tag="JN331_001501"
     CDS             23625..24953
                     /locus_tag="JN331_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HAY7259679.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVIGNASIMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSDVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVGQATTPAQSTCWPSATHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAETWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            24950..26029
                     /locus_tag="JN331_001502"
     CDS             24950..26029
                     /locus_tag="JN331_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAY7259680.1"
                     /translation="MNDNVTLRVNGREWNGWTLVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATTVRYDARSVITGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            26029..26577
                     /locus_tag="JN331_001503"
     CDS             26029..26577
                     /locus_tag="JN331_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HAY7259681.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     IEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPKARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMGA"
     gene            26577..27002
                     /locus_tag="JN331_001504"
     CDS             26577..27002
                     /locus_tag="JN331_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7259682.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            26989..28047
                     /locus_tag="JN331_001505"
     CDS             26989..28047
                     /locus_tag="JN331_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAY7259683.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFVRLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRLEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPITCSTTGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYLQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            28038..28622
                     /locus_tag="JN331_001506"
     CDS             28038..28622
                     /locus_tag="JN331_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HAY7259684.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAQSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRQDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNSTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            28626..>28961
                     /locus_tag="JN331_001507"
     CDS             28626..>28961
                     /locus_tag="JN331_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259685.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTLATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGAE"
BASE COUNT         6900 a         7036 c         8437 g         6588 t
ORIGIN      
        1 cagttggctg atgctcttcg agtgcgactt tctgaccaat tttctgaaat gtactctccg
       61 aatttggaca aatagaaaaa ggccatttcc attcagggcc tgatggaaag acttcagcct
      121 gttctaaagc acggcgtaaa gagaatacaa ctccagccat aatctgatgt ttcccattgg
      181 cccagctatc gccgctctga tctacatggg cggctatgtc gtatgaccaa acgacttcac
      241 agttattgtt taaaatctgg actttcattt catacacctg ctttaacatg agtacctagt
      301 ggcacaacat gactcaacga atcatcctgg acttcatatg ccccaggcgg ctacttcgtg
      361 ggcgtcctgc ctgtttgttg tttctcttgg gtacattatg tatctcatgg gtacattgtc
      421 aagtataaaa aaacctgccg aagcaggttc ataaacattg attaggcttt gattttgtat
      481 cttcttggtt ttcctgagaa aatcacagtt ccaattatag agcaattacc gttgatctta
      541 atgtaaggct caggccagtt tgggtttaac gctttgagat aacgctgtgt cccatcttct
      601 atcaaccttt tgaaggtggt ttcacctgta tcgtgcatca atgcaataac gtcgtcaccg
      661 tggcaggcag gtacttcagg atcgacaaaa atcatgtctc ccgggcggta ctcatcaatc
      721 attgaatcac ctatcacccg caagatataa gtcatttccc cacagggtac agggcaggga
      781 tacgtttctg ctgtgctcaa atcaacctca gaatatccaa cttctttcca tgctccggcc
      841 tgtacccatg atatgacagg gactaatgtg atttgtttat tagtgattga aacatcaggt
      901 ttttttgtga tgttcgttgt ctggtgttct tgatcgagcc atccgacagg caggtcgaaa
      961 catttttcga tgtgccgtgc catgctgtca ccgatatttt tagtagcgcc atcccccata
     1021 aacctgctgg tttgggttgg ctcgcgatca atcatggtgg caaaggatga atttccgcca
     1081 acaccatctc tcagttttct ggcgttagac cgccggatgt catggattgt tttcataacg
     1141 aaattaaaac ctttgtaccg ataaggtaca agtatcttga gggttcattt caatcatgta
     1201 atatgtatac cggaggtaca tattgtatga aagcgtattg ggactcttta accaaagaac
     1261 agcagggcga gttggccgga aaagttggct caacacctgg ctacttacgg ctggttttca
     1321 atggttataa aaaagccagt tttgtgctgg cgaaaaaact tgagcaatgc acgtcaggtg
     1381 caattacgaa atctgactta agaccggata tctatccgaa agattaacag aacaccttca
     1441 atttttaacc acagaacgat gaggctaacc gtgggtaagt atcactggaa agtagaaaaa
     1501 cagcctgagt ggtacgtgaa agctgtcaga aaaactatcg cagcgttgcc gggtggttac
     1561 gctgaagcag ctgactggct ggatgtaaca gagaacgcat tatttaaccg ccttcgtgcc
     1621 gatggcgatc agattttccc gctgggatgg gcaatggttt tacagcgcgc ggctggcact
     1681 cactacattg cggatgctgt cgcacagtct gctggtgggg tgttcgtatc gcttcctgaa
     1741 attgaggaag tagagaacgc cgatataaac cagcgcctgc tggaagtcat cgaacagatc
     1801 gggaattact caaagcagat tcgttcggca atcgaagatg gggtcgtgga gccacacgag
     1861 cagacagcaa ttaatgatga gttgtatctg tcaatttcga agctccagga gcatgcagca
     1921 ctggtctaca aaatcttttg cgctccagaa aagagtgacg cccgcgagtg tgcagctccg
     1981 ggcgtcgtgg cgttttgtgt ctgtggagaa actaacgcat gaacagttta acggcaaata
     2041 accgtttgtc gcaacagctg gtggtcagtg tcgctgaaca cctgttgtta cggcatgaat
     2101 gcagattacc aaatcacctg gctgtaagta accacagaga actttacctg actgtggggg
     2161 gcgagttgtg caggaactta accgctggtt tcgtgacgga agagggcttt atgtccatgt
     2221 tattcgttgg gagccagaaa cacagcgcgt tatctatctt cgcaaagact acccgcatga
     2281 gtgctttagt cctttgtgga aattcaggcg tgattttgtt gagtgtgaag gaccaccagc
     2341 acattgattc tgccattccg ggacgttaca ctgttcaggc accttataaa gcgggtgccg
     2401 ggattggcgt cctggaaatg ttatcggcga tatatgacgc gccagcgtct tttttatcgt
     2461 ctgcgtctgc gcacacccaa attatggtgg gctggacggg ggcactgaaa ggtgcgccgg
     2521 tttccgataa cgccggttac gccaaccccg ttcagttcac caccagcgaa attggcgttt
     2581 ccggtggtga aggtaattca ctgttatcgg aggctgccat catggctact gtcccagccc
     2641 tcactcgtct gaatgatgaa gacttacata aactcagtta tgtaacaact gcactacgtg
     2701 ctctgcgcaa ggtaactctt tcggatccgc aggcgcatca ggttctggta gaaacccttc
     2761 ttaacttgca ggctgaacgt atccgtttgg cgcataaggc taattttcat attcaccgtc
     2821 tcctgaatat cagcggaggg catcgtcatg cttaatccgt tgatcctcaa tatttgccgt
     2881 ttgcttcagc gtaaaaaaac atcaattcct acagttgggc agtggtacac cacgcctgca
     2941 gggcatgttc tacgtgttag cctggttgac cgtgaatgtc agaaggtggt ttgtgaaccg
     3001 ctgggccgta attaccgcgt cagtatgccg cttatagcct ttcgctccgg aaaaaacatg
     3061 aagcatctcg gaggtgcagc atgagtatgg agctgatggt taaagcgatg aaaattcgag
     3121 tgggtaatcc attgcgaaaa ctggttctga tcaagctggc tgataatgcc agcgatcagg
     3181 gtgagtgctg gcccagctac cagcatattg ctgaccagtg cgagattagc aaacgttctg
     3241 tgatgaatca tattgcggcc ctttgtgagt ccgggctggt aaaaaaagtc acccggaaag
     3301 gtgaaaaagg taactcaagt aatatctatc tccttcatct tgatggtgca ggagattcac
     3361 taggggtagt gcaaataatt tactatctgg tgcagcaaat tcactaggta gtgcaggagt
     3421 tgcaccaggg ggtagtgcag gagattcacc cagaaccagt cactcttttg aaccagtcaa
     3481 agaaccagtc aatgacccaa tagctgttgg tgcatctgct gatgagtctg tgcgagttcg
     3541 ttcaaaccga ccggaatact ctccggagtt tgagcaggca tggctggcct atcccaaacg
     3601 tgctggtggc aattcaaaat ctgcagcctt caaagcctgg aaagcccgtt tgaatgaggg
     3661 ggtaaacccc gaaaccatgc tggaaggtgt gaaacgctac gcgggctggg tatctgcgat
     3721 gggtaacagc ggcacacaat ttgtgaaaca ggctgtcacg ttctttggtc cggatcgtca
     3781 tttcgaagaa tcctgggaag ttcctgcagt atctgcagcc agacgcgagg acccgtactt
     3841 caaagccagt tacgacaacg tggactacag ccagatcccg gcaggattca gggggtgatt
     3901 atgagtcttt tgaatgaagt tcagaaattc attgaagccc atccggggtg tacttccgga
     3961 gacattgcgg atgcttttgc tggttactca cggcagcgcg ttctgcagtc agcaagcaag
     4021 ttacgtcaga gtgggcgtgt ggctcaccgt tgtgaaggag atacacgcag acatttcccg
     4081 cgcctgactg agagagcgca ggagccggaa ccacaatctg ttcgtgaaac cagacctgtg
     4141 cgcaatttct atgtcggcac taacgacccc cgggtgattt tgtgcctgac ccgccaggct
     4201 gaagaactgg agtccagggg cttattccgt cgagctgcaa cggtgtggat ggaggcattc
     4261 cgtgaaagcc actcccagcc agaacgaaac aattttctgg cgcatcgtga gcggtgctta
     4321 cggaaaagca gcaagcgcgc tgcatcgggt gaagagtggt atctgtcagg gaattacgtg
     4381 ggggcttaat gagtaataaa tattgccagg cgctagtgga gctgcggaac aaaccagccc
     4441 atgaactgaa ggaagtgggc gatcagtggc gcacgccgga caacattttc ttgggaatta
     4501 acaccctgtt tggcccgttt gttctggatc tgtttactga tggtgataac gccaaatgtg
     4561 ccgcttatta cactgcggaa gacaacgcgc tggcgcatga ctggtcagaa cgtcttgcgg
     4621 agcttaaagg tgctgccttt ggtaatcccc cgtacagccg cgccagtcag catgaggggc
     4681 aatacatcac cggcatgcgt tacatcatga aacatgccag tgccatgcgt gataaaggtg
     4741 ggcgctatgt tttcctgatc aaagctgcca ccagcgaagt ttggtggccg gaagatgcag
     4801 accatattgc ttttattcgc gggcgtattg gttttgaact gccagcctag tttatcccga
     4861 aggacgagaa gcaggtgccg acaggcgctt tcttcgctgg tgctattgct gttttcgaca
     4921 agacctggaa gggaccggca atcagctaca tcgggcgcga tgaacttgag gcatgtggtg
     4981 aggcctttct ggcgcaggtt cgccagcagg cggaaaaact ggtcagggag atggcggcat
     5041 gacgacatta actcaatgcc agcagcaggt gctggatatg ctgatttctt accagaaaga
     5101 acgtggcttc ccgccaacca atcaggaggt ggcaaccatg ctgggatacc gttcagtgaa
     5161 tgcagcggtg gagcatcttc gcgcactgga gaaaaaaggc gtcatcacga taaagcgtgg
     5221 cgtggcccgg ggcatcacgc ttcataccgc ggtgaaggac gacgacagcg aggcggtcgg
     5281 gattatccgc tcactgcttg ccggtgagga aaacgccagg ctgcgtgcaa cccactggtt
     5341 acataagagg ggcctgaaag tatgaagctg atcctgcctt tcccgcccag cgtgaacacg
     5401 tactggcgac accccaacaa aggggcgttt gcaggtaaga gcctgataag cgcggcgggg
     5461 cgaaaatttc agagcgcggc gtgcgcagca atagttgaac agttacgtcg tctgccgaaa
     5521 ccaacgtcgg cacctgcttc agtggagatc gtgttgtttc ctccggataa ccggatccgc
     5581 gatctggaca actataacaa ggcgctgttt gacgccttga cccacgcggg ggtgtgggaa
     5641 gacgacagtc aggtgaaaag aatgctggtg gagtggggac cggttatccc gaaagggaag
     5701 gtcgagatca ctatcagtaa gtatgagaaa ccggcgggtg cagccgcctg attaagagga
     5761 gaaacgaagt atgaataatc tgatggtcat tgatggtatt gaagttcgtc gtgatgctta
     5821 tgggcgttac agcctgaacg atctgcatcg cgcagcagta gcatctggtg caaatgccag
     5881 aaccaaggag ccaggaaagt ttctttccag ccaacaaact gttgagcttg ttcatgaatt
     5941 gaccaacacc cagaatttgg gtgttgaccc ggtgagtgtg attcatgggg gaaatgaacg
     6001 gggaacgtat gtctgcaagg aactggtgta tgcctatgca atgtggatca gcccgtcatt
     6061 ccatctgaag gtgatccgta ctttcgatat ggtaaccagc gcaccggaaa aattatccgg
     6121 gcaggctgct gacaagatgc aggctggtgt gattctgctg gactttatgc gtcgggaatt
     6181 aaacctgtct aactcatctg tgcttggggc ttgtcagaaa ctccaggagg ctgttggctt
     6241 accgaatctg gctccgcgct atgcaattga tgctcctgcc gatgcacccg atggctcaag
     6301 tcgccctacg ctgtcactga gtgcactgct gaagcagtat ggtatccgcc tgacggctaa
     6361 tcaggcatat caccagatgg tgaagctggg gatcgtcgag cagcgcgaac gatacagccg
     6421 taccgcgatt aacaacatca aaaaattctg gtcgctgaca gcgaaaggct gcatgttcgg
     6481 caagaacatc accagtcccg caaatccgcg cgagacgcag ccgcatttct tcgaatcccg
     6541 attccctgag ctgttaaagc tgctcgatac cgttcattga ggtgaccgtg agagcattac
     6601 tgacccctga aattgccccg cgtatgggga tcgtattgtt cagacccggt tcagagctga
     6661 tgcccctgtt tatgcagggg cgtgtcctgc tggagcctga gccggaacgt tattcatctt
     6721 ttgccagtgg tgccgttccg gcggcatcac aaccgctggc ggatgatcct gctgttcggg
     6781 ccgtgttccg caatgaggca gtgatccgtc gtgctggtgg cgtggaatgt cttgaaagct
     6841 ggttacttcg tgaaaaaggc tgctagtggc ctcattccga ctggcacagc gagaacatga
     6901 caacaatgcg acacgctccg ggcgcaatcc gtctgtgctg gcactgcgat aaccagctgc
     6961 gcgatcagtt cacggaacgg ctggaatcaa tggcaacgga taactgtgcc cgctgggtgt
     7021 tgtctgttgt gcgtcgggat ctcggttttg atgacagtca cgttgtgaca atgccggaac
     7081 tgtgctggtg gctggttcgt aatgatctgg cggatgcctt accggaaagc gcagcccgta
     7141 aggcactgag attaccgaat cctgttgtgc cgtctgtcac ccgggaaagt gaccttgtgc
     7201 cttcggttcc tgccaccagc atcatccagg ataaggcgaa aaaggtgctg gcgctgaaag
     7261 tggatccgga gtcgccggag tcttttatgt tacgcccaaa acgtcgccgc tgggttaatg
     7321 aaaagtacac gcgctgggtt aagacacagc cgtgcgcatg ttgtggaaag ccagccgacg
     7381 atcctcatca cctgattggt catggtcagg gtggaatggg tacaaaagcg catgacctct
     7441 ttgtgttgcc tttgtgcaga aagcatcacg acgagctgca tgcggatacc gtggcatttg
     7501 aagagaagta tggctccctg ctggagctga tatttcgttt tatcgatcgt gcgctggcaa
     7561 taggcgtact ggcgtaagtg gagaacgagc atgaaccttg aagccttacc aaaatattac
     7621 tccccaaaat ctccaaaact gagcgatgac gcaccggcga caggctcggg tggtttaacg
     7681 attacggatg tgatggctgc gcaggggatg gtgcagtcga aagcaccgct tggatttgcc
     7741 ttattcctgg caaaagttgg tgttcaggat cctcagtttg cgattgaagg tctgctcaat
     7801 tacgcgatgg cactggataa tccgacattg aataaattga gtgaagaaac ccggttacag
     7861 ctcatccctt accttgtgaa ttttgccttt gctgattatt ccaggtctgc ggcaagtaag
     7921 gctcgctgtg agcattgtgc tggtactgga tttcataatg tattgcgcga agtggtgaaa
     7981 cactccagaa gcggtgaatc tgttatcaag gaggagtggg tgaaggaact atgtcagcat
     8041 tgtcatggta agggagaagt cagcacagcg tgcagagggt gtaagggtaa aggtattgtc
     8101 ctggatgaaa aaaggacccg gcttcatggc acgcctgttt ataagatttg tgggcgttgc
     8161 aatggaaacc ggtttagccg tttgccaacc acactggcgc ggcatcatgt ccagaagctg
     8221 gtaccggacc tgacggatta tcagtggtac aaaggatatg cagatgtcat tgataaactg
     8281 gttacaaagt gctggcagga agaagcatat gcagagacac aattgagaaa agtgacaaga
     8341 caaatgattt tcgccgaaga tggcgacatg atgcttgcat ttttcaaaaa atatggttag
     8401 gattctccta acgatgggct ttgtatgtct gccgttaacg aaatcataac aaacctcgct
     8461 tcggcggggt ttttgctttt ctggaggtca ataatgcagg gcgaaaagca gcagccatat
     8521 ttttttaacc ctggtatgac tgttgaacag cttgaagact ggctggagca gcaaaagctt
     8581 catctaagcc gctataaccg tctggtaaaa gaaaaagcag agcttgaaga acggctcagt
     8641 gatatttctg tggaaattga acgagtgtct gctggtggtt ttaacggaaa gttgagtttc
     8701 ccctgggagt caagttcgct tctgagaaat catcaacagg gtagtgtttg actgaaataa
     8761 taaatagact gtcattaaga tcccttcccc tcatatctga gaggaccaac agcaattaag
     8821 agggggctaa atgtccgatc cgatttccgg tactgggctg gctggtggtg ccctgacggg
     8881 tgccagtgtt tatggactgc tgaccggaac tgattacggc gttgtatttg gcgcatttgc
     8941 aggggctgta ttctacatag caacagcagc agatctgagt gcatcgcgcc gactggcata
     9001 ttttatcgtg tcatatattg ccgggatcct ttgctctggg ttggttggct ccaagctggc
     9061 gcacttgacc ggatacagtg ataaacctct ggatgctatt ggtgccgtaa tcgtctctgc
     9121 tttaaccgtt aaaatcctga cgttcctgaa taatcaggat atcggctcgc tggtggcgct
     9181 cataacgcgc cggggaggtt caggtggagc taaatgaccc gacagcaact ataaatgcgc
     9241 tgttatgtgc ttgtgttgtt attactctga tgttttatcg tcgtggtgat tcgcggcatc
     9301 gtccttgggt ttcacgttta gcctggctga ttactgttac atacagtgct gttccgttgg
     9361 cctatctctg tgggatttat ccccattcct catggcccat tatcgtggcg aacactattt
     9421 ttctttccgt gctggtggcc gtcagaggca acgttgcacg tctggttgat catctgaggc
     9481 actaatgaac caacaattat ttcaaaaggc ggctggtatt agcgccgggc tggctgcgcg
     9541 ctggtttccg cacattgatg ctgcaatgaa agagtttgga atcacagcag ttaacgatca
     9601 ggccatgttc attgcacaag ttgggcatga atctgctggt tttacctcgc tggtggaaag
     9661 cttcaactac tcggtagacg ggctgaagaa aacctttagt aaacgcctga cgccttatca
     9721 gtgtgaaatg ctggggcgtg tcgatggtaa gcaggtagcc caccagccgc aaatagccaa
     9781 tctggtttat ggtgaccgca tggggaataa cagccagggt gacggctgga aatatcgcgg
     9841 tcgtggcctg cttcaaatca ccggccgcga gaactacgcc aaatgcggtg cggcgctgaa
     9901 gttggatctg atcagcacac cagagttgct gacacaggag aaacatgcag cccgttctgc
     9961 tgcatggtat ttcacattac gtggttgtct gatgtattca ggtgatgttg tccgtgtaac
    10021 gcagatcatc aacggtggcc agaatggact ggctgacaga aatagtcgtt ataacaaagc
    10081 gcgggcggcg ttgctggtat gacagcggtc tttgcttttg ttcaggcgcg gtggaaaaca
    10141 atcattgttt tgctgatgtt ggctggtgca tttcttgccg ggaacatctg gagtgagcgg
    10201 ggctggcaaa agaagtgggc tgaccacaat agcatggaat cttcacagga agcgaacgcg
    10261 cagactgccg cacgctggat tgaacaaggg cgcataattg cccgtgatga ggctgtaaaa
    10321 gatgcacaag cacaagccgc taaatctgct gccactgctg ctggcctgtc tgccactgtt
    10381 agccagttgc gtaccgaagc aacaaagctt gccgcacgcc tggacgccgc aaagcacacc
    10441 gctgatcttg ccgctgccgt ccgaagcaaa acaaccgacg ccggaatgtt cgccgacatg
    10501 ctcggagata ttgcagcaga agctaaacgt tatgctggaa tcgctgacga acgctacacc
    10561 gccgggatga cgtgtgaacg tatttacgac tcgttgagag agtcaaataa cgctatcccc
    10621 cacaattgat tttttatcat attcgaacga ctatgaaggg tgtaaacaat tataatccag
    10681 tgatgaattt ttcaaatgtc aaagcaagga tatgcagtga gtggtcatag tgatggttta
    10741 gcagatattt tgagcattgt aattgtatta gcagtggaac attggattat cgcatctctc
    10801 atagttggtt tgattatgta tctagtgggg gcgagcataa acgaaaatgc tcagacaaat
    10861 gccggtgcca ttgcatcggg aatcataggg gtatgtggtc aatttatttt tggttttggc
    10921 gttctggcta gcattattgg gattgttaaa ttcatttgga cccatacctg acagcatcac
    10981 tttagtcaat catagcctcg caatagcggg gctttttaac aactgaggta tgagcatgac
    11041 agtagttctt acagctaagc agattgaaga cctagcagca ttcgcgaaag aagatggtca
    11101 gccccaatac accatcacca ctggtacaat cccggaattc gaagcggatg atggcgagat
    11161 tatccctgaa tacacagggc tgatcgctta ctcagagtca cttgagcacg gtgtattgca
    11221 actcgacgat tagtggcatt cagcaggcat tcattaagtg tctgcgataa tgctatacgt
    11281 tttttgggga gtgaatatgc caccgcgcac accaaaggct tgccgtgttc gcggctgtcg
    11341 caacaccaca acagacccat ctggctactg tgaaaatcac aaaggtgaag gctggaagtc
    11401 ctacaagcct ggtcaatcac ggcagcagcg aggatacgga acaaagtggg aagtcatccg
    11461 ggagcggata cttaagcgcg acaaagggct gtgtcagaac catctacggc aggcgatagt
    11521 gaagcaggcg tcctgcgtgg accacatcaa ggcgaaggcc cacggcggta ctgatgaaga
    11581 cagcaacctt gaaagcctgt gctggtcgtg tcacgcagcg aagaccgcgc gtgagcggct
    11641 caagtgataa ttaatgtcat catcagactg gggaggggga ggtcaaatct ctgtgaccgc
    11701 gcgccttccg gactgcccgc cccatcgttt ttttataccc gcgaaaaatg aaatttaacc
    11761 aggagtgccg catatggctg gaacggcggg gcgttccggg cgtcgcccca agccaacggc
    11821 gcgcaaggcg ctggccggaa accccggcaa gcgagccctg aataaagatg aacctgtttt
    11881 tacgcccatc aaaggtgttg agccaccgga gtggttcgct gaagaagatc tccctctcgc
    11941 tacgatcatg tggcaactga caactaaaga actctgcggt cagggcctgc tgtgcgtgac
    12001 tgacctcgcg gtgcttgagc ggtggtgcgt ggcctacgag ttctggcgac gtgccgtgaa
    12061 aaatattgcc agacagggca acaccatcac cggtgcaatg ggcggtatgg tcaaaaatcc
    12121 ggagctgacc gccaaaaaag aacaggagtc cgagatgagc agtacggggg caatgctcgg
    12181 actcgacccc agcagccgcc agcgtctgat tggcctggcg gggcagaaga aagccactaa
    12241 cccgtttctg aaaatcatcg aatcatgagc cggaaatctt accccaacgt aaatgctgcc
    12301 aatcagtatg cccgtgatgt cgtgcgcgga aagattgtgg cctgccagtt tgtgattcag
    12361 gcctgccagc gccatcttga tgacctgatg gcggaaaaaa gtaagtcgtt tcgttaccgc
    12421 ttcgacaagg acctggctga acgggccgcg aaatttattc agctgttgcc acacaccaag
    12481 ggtgagtggg cattcaaacg gatgcccatc acgctggagc cgtggcagct ctttgtgatc
    12541 tgctgcgcgt ttggctgggt caataaaggc tcccggctgc gccgcttccg tgaggtgtat
    12601 accgaaatcc cccgtaagaa cggcaaatcg gcaatctctg ccggtgtcgc cctgtattgt
    12661 tttgcctgtg ataacgagtt cggcgcggaa gtgtattccg gtgccacgac ggagaagcag
    12721 gcatgggaag tctttcgtcc ggcacgactg atgtgtaaac gcacccccat gctgacggaa
    12781 gcgttcggga ttgaggttaa cgcctcaaac atgaatcgtc cggaggatgg cgcgcggttt
    12841 gaaccgctga tcggtaaccc cggtgatggt tcatcacccc actgtgcggt ggtggatgaa
    12901 tatcacgagc acgccaccga tgcgctttac accacgatgc ttaccgggat gggggcgcga
    12961 cgtcagccac tgatgtgggc tatcaccacc gccgggtaca acattgaggg gccgtgctac
    13021 gacaagcggc gggaagtcat cgagatgctc aacggctcgg tgcctaacga tgaactgttc
    13081 gggatcatct ataccgttga tgaaggtgac gactggaccg acccgcaggt gctggaaaaa
    13141 gccaatccaa atattggcgt gtcggtttat cgcgaatttt tgttaagtca gcagcagcgt
    13201 gcgaaaaata acgcccgtct ggcaaacgtc tttaaaacaa aacacctcaa tatctgggtg
    13261 tcggcgcgtt cggcgtattt caacctggtg agctggcaga gctgcgagga taaatcactg
    13321 acccttgagc agttcgaggg gcagccgtgc attctggcct ttgacctggc gcgtaagctg
    13381 gatatgaaca gcatggcgcg actttatacc cgcgagattg acggtaaaac gcattactac
    13441 agtgtggccc cgcgtttctg ggtaccgtat gacgcggtgt atagcgtcga gaaaaatgaa
    13501 gatcgccgga cagccgaacg ctttcagaaa tgggtggaaa tgggcgtcct gaccgttacc
    13561 gatggtgcag aggtggatta tcgctacatc ctcgaagagg ccaaagcggc gaacaaaatc
    13621 agcccggtca gcgagtcacc catcgacccc ttcggggcga ccgggctgtc acatgacctt
    13681 gctgatgaag acctgaaccc cgtcaccatc attcagaact acaccaacat gtccgacccg
    13741 atgaaagagc tggaagcggc aattgaatcg gggcgctttc atcatgatgg caatcccatc
    13801 atgacctggt gtatcggcaa cgtggtcgga aaaaccattc cgggtaacga tgatgtggtg
    13861 aagcccgtca aagagcaggc ggaaaacaaa atcgatggtg cagttgcgct gattatggcg
    13921 gttggcagag ccatgctgta cgagaaagaa gacacgctgt ctgaccacat tgagtcctac
    13981 gggatccgct cgctttaact gaggtaatta tgatcatgct gattctcgcg cctctggtag
    14041 gcgtgctggg ggcgcttttg ctggcgtatg gtgcctggct gatttatccc ccggtgggtt
    14101 ttgttgttgc cggggcgttg tgcctgttct ggtcgtggct ggtggcgcga tatctcgacc
    14161 gtacacagca gtctgtcggc ggaggtaaat agtgttcttt tcgggattat ttcaacgaaa
    14221 aagtgacgca ccggtgacca cgccagcaga gctggcggat gctatcgggt tgtcctacga
    14281 cacctatacc ggaaagcaga tcagcagtca gcgggccatg cgactgacgg cggttttttc
    14341 ctgtgttagg gtgctgacgg agtcggtcgg gatgttgccc tgcaacctgt atcacctgaa
    14401 tggcggtctg aagcagagat ccactggcga acgtctgcat aagctgatct cctcgcatcc
    14461 taatggctat atgacgccgc aggagttctg ggagctggtg gtcacctgtc tgtgcctgcg
    14521 gggcaacttt tacgcctaca aagtgaaagc atttggtgaa gtggctgaac tgctgcccgt
    14581 cgatcccggc tgtgtggtac cgaagcttaa cagtagctgg gagccggtct atcaggtcac
    14641 attcccggac ggctcaacgg atgtgctgac ccaggaggat atctggcatg tgcgcacgct
    14701 gacgctggac ggtctggtgg gactgaatcc cgtcgcctat gcccgcgagg caatattgct
    14761 ggcagcagcg actgaagaac acggtgccag actgttcagc aatggcgcgg tgacgtccgg
    14821 tgtgttgcgt acagaacaga cgctgtcgga tcaggcttat gagcgcctga agaaagattt
    14881 tgaggagcgt cacaccgggc ttggcaatgc tcaccgcccg atgatccttg agatggggcc
    14941 ggactggaag tcgatggcat tgaacgccga ggacagccag ttcctggaaa cccgcaagtt
    15001 tcagcttgaa gaaatctgtc gtctgttccg ggtgccattg catatggtgc agaacaccga
    15061 tcgcgccacc ttcaacaata tcgaagagct ggggctggga tttatcaact attcactggt
    15121 gccgtatctg acccgcattg agcagcggat caacaccgga ctggtacgaa aaagtaagct
    15181 gggcatttat tacgccaaat ttaacgccgg ggcgttactg cgcggggata tgaagtcccg
    15241 ttttgaagcc tacgccaccg ggatcaactg gggaatttac tctcccaatg actgccgcga
    15301 cctggaagat atgaatccgc gtcccggtgg tgatgtctat ctcacaccga tgaacatgac
    15361 cacgaaaccc tccgatggca gtaaagccgg taagcagaag gataacgcca atgcagacga
    15421 aacaacgtct tgatgtaccg ctgagtctga aatctgtcag tgactccggt gagtttgaag
    15481 ggtatggctc cgtctttggt gtaaaggaca gccacgatga tgtggtgatg tccggggcat
    15541 ttgctgcttc cctgcgggcg tggagtgaca gaaaagcgtt acctgcgctg ctctggcagc
    15601 accgcatgga tgaacccatc ggtgtttata ccgaaatgaa ggaagacgat gtcgggcttt
    15661 acgtcagggg acggttgctc attgatgatg atcccctcgc aaaacgcgca catgcacaca
    15721 tgaaggccgg ttcgttaacc ggcctttcta ttgggtacgt cctgaaagac tgggaatacg
    15781 accggagtaa agaagccttt ctgctgaaag aaatcgacct ctgggaagtc agtctggtga
    15841 cgttcccgtc taacgacgag gcgcggatca gcgacgtcaa gaacgcgctg gcccgcggtg
    15901 aaatccccga acagaaaaaa atcgaaagag tcctgcgtga tgtcggactc tcccgtaccc
    15961 aggccaaagc attcatggcc gggggctatg gcgcactgtc cctgcgcgac gctgaggatg
    16021 tgggctctgt actgaatgca ctgaaaaatc tgaacttcta atcaggagaa atacgatggc
    16081 ggttgatatt aaagatgtgg aacaggtcgc gcaggagctg cagcagaagt ttgacgactt
    16141 caaagcaaag aacgacaagc gcgtggatgc gattgagcag gaaaaaggca aacttgccgg
    16201 gcaggtggaa accctgaacg ggaaactcag cgagctggaa aacctcaaaa gcgatcttga
    16261 aaaagagctg cttgagctga aacgtccggc aggtggtgcg caaaataaac tggccaccga
    16321 gcataaagaa gcgtttgtgg gcttcctgcg taaaggccgt gaagatggtc tgcgcgatct
    16381 ggagcgcaag gcattacagg tgggcaccga tgaagacggc ggctatgccg tgccggaagc
    16441 actggatcgc aacattctca ccctgctgaa agatgaagtg gtgatgcgcc aggaagccac
    16501 ggtgatcacc gttggcggtt ccgactacaa aaaactggtg aatctgggcg gcacggcttc
    16561 cggatgggtt ggcgagactg acgcgcgctc ccagactgcc acctcaaaac tgggactgat
    16621 tgaacctttc atgggggaaa tctacggtaa cccgcaggcc actcagaaaa tgctggatga
    16681 taccttcttc aacgtggagg cctggatcaa cagcgagctg gcgaccgaat ttgccgaaca
    16741 ggaagaaatt gcctttacca ccggtgatgg caccaagaag ccaaaagggt tcctggcgta
    16801 tgaatccacc gatgaaaccg ataaggttcg tgcgttcggt aaacttcagc atattgtatc
    16861 cggtgaagcg acgacggtga ccgcagacgc cattatcaaa ctgatttaca cgctgcgtaa
    16921 ggcacaccgc actggcgcga agttcatgat gaacaacaac agcctgtttg ccatccgtct
    16981 gctgaaagac accgagggta actatctgtg gcgtccgggg ctggagctgg ggcaaccgtc
    17041 ctctctggcg ggttacggta tcgctgaaaa cgaacagatg ccggatatcg ccgctgatgc
    17101 gaaagctatt gcatttggta acttcaagcg gggttacacc atcgttgacc gtatcggtac
    17161 ctgcattctg cgtgatccgt acaccaataa accgtttgtc ggtttttata caaccaagcg
    17221 caccggcggc atgctggtcg attcgcaggc catcaaactg ctgaagattg cagcggcgta
    17281 atcactcagg ggcgcggaac cgcgccccct gttctgacag gtgaaagaat catgatcctg
    17341 aaacaagatc tgaaatggtc accggacggt atgcgtgttg aggtcattca ggccggtgag
    17401 tatgacgacg gggcgcttcc tgcccgggtg caggagattg cacttcaggc cgggttagca
    17461 gagcgcggaa tcagtgcaaa aagcagtaaa gcggcaaaag agaaaaaagc cacgaccagt
    17521 aaagagggct gagtatgctt ctgacaatgg aagagattaa agcccaactc cggctggatg
    17581 aggatttcga tactgatgac cgccatctgc aactgctggc atgtgcggca caaaagcgga
    17641 cggaaacgta tctgaaccgg aagctctatg caccggatga aaccattccg gacagcgatc
    17701 cggacgggct gcacctgccg gatgatattc gtctggggat gctgatgctt atcagccatt
    17761 tttacgaaaa ccgctcgtcg gttacggaag tggagaaact cgacatgccg cagagttttg
    17821 gctggcttgt cggcccgtac aggtactttc cgcaatgaaa attcgtcagg cgcagaccag
    17881 cgcaacctac attctgccgg accccggtga actgaataaa cgcgtcctga tccgcctgcg
    17941 ggtggatatg cccgcggata actttggcgt ggagcctcaa tacccggtta cgttccggac
    18001 atgggcgaag gttatccaga ccagtgccac cacctggcag taaaccgcgc agaccgggga
    18061 cgccatcacc cattacatca ccattcgtta ccgccggggg atcaccgctg attatgaggt
    18121 ggtctgcggt gacagtgtgt accgggtgaa acgtcagcgc gatctgaacg gggcgcggcg
    18181 ctttctgctg ctggagtgta cggagctggg cgaatgtagg cagagtcacg gaggcaacaa
    18241 tgacgacttc ctttttgcac gttgattttc agcagcccgc ggagatgcgc tttaaccgcg
    18301 cccgtgtccg gcgggcgttt gtcacgattg gtcagcgtca tatgcgtgat gcccgtcggc
    18361 tggtgatgcg ccgtgcgcgg tcggcaccgg gtgaaaaccc cggttatcag accggacgcc
    18421 tggctcgttc gattggttac atggtaccca gagccagtaa acatcgccct ggttttatgg
    18481 cacgtatagc ccctaaccag cgtaatggag agggaaaccg ccgtatcacc ggtgattttt
    18541 atccggcttt tttgttctat ggcgtgaggc gaggggcaaa gcgtcgtcgc agccatcatc
    18601 gtggtgcatc cggtggcagc ggctggcgac tggctccacg taataacttc atggtggaaa
    18661 cgcttgaaaa gaaccgcagc tggacacgct attttctggc gcgggaattg cgtaaatcac
    18721 tgaagccgga gcgacgacac agatgaaact gacgcctgtt attgctgcac tgcgtgcccg
    18781 ctgtccgtat tttgaaaacc gggttgcagg cgcggcccag ttcaaaaatc tgccggaggt
    18841 cggaaagctg aaactcccgg cggcatatgt tgtaccgggt gatgattctc cgggagaaaa
    18901 caaaagccag accgactact ggcaggagct gaaagagggt ttctccgtgg ttgtcatact
    18961 gagtaacggg cgtgatgagc gcggtcagtt tgcctcgtat gatgtggtgg acgatgtccg
    19021 gcagatgctc tttaaggctc tgctgggctg gaacccggag gcgtgcggta acccgattac
    19081 ctatgacggc ggcacgctgc tggatctgaa tcgtcatgag ctgatttatc agttcgattt
    19141 ttcggtcatc agcgagctga ctgaagacga tacccgccag caggatgatc tgaacagtct
    19201 ggatgaactg caaacgctgg cgattgatgt tgattatctc gagcccggta acgggcctga
    19261 cggcgatatc gaacatcaca ccgaaataac ccttccttcc tgaggatcct catgtttgtc
    19321 aaacctgtta aagggcggtc agttcctgac cctgcccgcg gcgacctttt gcccgccgaa
    19381 gggcgaaatg ttgacgagaa caactactgg ctgcgccgtg aagcagcggg tgatatccgg
    19441 cgcgtgaata aaaaggtgaa taccgatgac gataagcttt aacaccattc cgtcgaatac
    19501 gctggttccg ttgttttatg cggaaatgga taactcggcg gcgaatactg cacaggacag
    19561 cggagcatcg ctgctgattg gtcatgccaa taacggtgca gagattgttg ccaacagtct
    19621 ggtactaatg ccgtcggcag actatgcacg ccagatttgt ggtgcgggaa gtcagctggc
    19681 gcgtatggtc gaggcttatc gccagaccga cccgtttggt gagctgtatg tgattgccgt
    19741 tccggaagcc acaggcgcgg cggcaacggt tacgctgacg gtgaacgggg cggcaaccga
    19801 aaccggcacg gtgaatgttt atgtgggacg tacccgcgtg caggcaccgg tgaccaacgg
    19861 cgataacgtc acgacgattg ccagcagtat ccaggatgcc atcaatgccg ttccggccct
    19921 gccgtttacg gcctcatctt cggcaggcgt ggtcacactg accgcgcgtc ataaggggct
    19981 ttgcgggaat gaaattcctg tcagcctcaa ttactacggc tttggtgggg gcgaagtgct
    20041 gccagcgggc gtacagattg ccgtggcgac ggggaccgcc ggaacgggcg ctccggttct
    20101 caccggtgcg gtggctgcaa tggcggatga gccgtttgat tatatcggtc tgccgttcaa
    20161 cgacacggcc tccgttaaca cgctggtgac cgagatgaac gataccagcg gtcgctggag
    20221 ctatgcgcgt cagctgtatg gtcatgtgta tacggcaaag atcggcacgc tgtcagaact
    20281 ggtgaccgca ggtgaccagt ttaaccagca gcacatcacc ctggcggggt acgaaaaaga
    20341 gacccagacg cctgccgacg agctggcggc aagccgtacc gcccgcgcag cggtgtttat
    20401 ccgcaacgat ccggcatgtc ccacgcagac cggtgagctg gtgggtatgc tgcctgcgcc
    20461 gaaggggaaa cggttcacga tgaccgaaca gcagaccctg ctgtttcatg gcgtggcaac
    20521 ggcgtatgtc gaaagcgggg tgctgcgcat tcagcgtgat gtcaccacgt acaggaaaaa
    20581 tgcttacggg gttgcggata acagctacct cgacagcgag acgctgcata ccagtgcgta
    20641 tgttctgcgc aaactgaaat ccgtcattac cagtaagtac gggcgtcaca agcttgccag
    20701 cgacggtacc cgctttggtc ccggtcaggc gattgtcacc ccggcggtga tcaaagggga
    20761 actgctggca acctaccgtc agcttgagcg tgcggggatc gtggaaaact acgaactgtt
    20821 taagcagtac ctggttgtgg agcgtgatgc cagcgatccg aaccgcctga acacgctgtt
    20881 cccgcctgac tatgttaacc agttgcgtgt ctttgccgtg gttaaccagt tccgtcttca
    20941 gtattcagag gagtctgcat aatggcccgt atcgggggaa cctgttattt caaaatggac
    21001 ggtcagcagc tatcgctgac cggcggcatt gaggtgccca tgaacaggac ggtcaatgat
    21061 gacatcatcg gcctggacgg ttcagtggac cgcaaggaaa ctcaccgtgc gccttatgtc
    21121 aaagggacct tcaaggtgcc gaagaatttt ccggtgagca aaatcacctc gtctgatgag
    21181 atgaccatca ctgccgagct ggcgaacggt caggtctatg tactgtcgtc tgcctggctg
    21241 cacggcgaag cgaaccataa tgccgaagaa ggcacggtcg atcttgagtt ccacggtgaa
    21301 gaaggggatt accagtaatg aaagagcttg agttaaagaa accgattacc gctcatggcg
    21361 agacactctc cgtactggag tttgatgagc ccaccgggaa ggatgtccgc gagctggggt
    21421 atccctacca gatgaatcag gatgagtccg tcagacttct ggcgcatgtg gtgtcgaaat
    21481 atattgtgcg gctggcgaaa gtgccgcaaa gctctgtcga ccagatgtct ccggcagacc
    21541 tgaatgcagc ggcgtggctt gtggctggtt ttttcctcca ggcctgacgg ctgaatacct
    21601 tactgatcgc ttctttgatt gcgccagcta ctggcgcatt aatcctttcg aattgctgaa
    21661 tatgccgatc agtgaaattc ccttactggt cagtcaggca aacaggatag agcaggagaa
    21721 acgcacacat ggctgaattt gagcttaagg cgttgatcac cggtgtcgac aggctttctc
    21781 ccgcgctgtc gaaaatgcaa aagaaaatcc ggggatttaa acgccaggcg gaagaagcgt
    21841 cacagggtgg gctggcgctt ggtggcggac tggcagcggg tctgacgctt tccctgaaat
    21901 cttatgccga tcaggaaaac gccgccaccg ggctgaaagt cgccatgatg gatgcgaacg
    21961 gcgaggttgg aaagagcttt caggacatca ataaactggc tattggcctg ggtaaccagc
    22021 tacccggtac aacggctgat ttccagaaca tgatgcagat gctggtgcgt caggggagcc
    22081 cggcagaaaa cattcttggc ggtgtgggta aagcgacagc ttatcttgcg gtacaactga
    22141 aaaaaacacc ggaagcggct gctgagtttg ctgcaaagat gcaggatgct accggaacgg
    22201 cctcagaaga catgatgggg ctgttcgaca caattcagaa ggcgttttat ctgggcgttg
    22261 acgacaccaa catgctgtca ttcttcacta aaaccagctc tgttctgaag atggtgaata
    22321 aggacggtct tcaggctgca cagagtcttg cccccatcag tgtcatgatg gatcagatgg
    22381 ggatgaacgg ggagtcggca ggtaatgccc tgcgaaaagt tatccagtcc ggattaagcg
    22441 ttaagaaaat cagggacgtc aataaaatca tggcccgcca gaaactcggg gtacagctcg
    22501 attttactga tggcaaaggg agttttggcg gccttgataa catgttcagg caactggcaa
    22561 agctgcgaaa actgaccgac gttaagcgaa ccggtgtact taaggcaata tttggtgatg
    22621 atgccgaaac ccttcaggtg gtcaatgcgc tgatcgataa aggaaaggat ggctacgatc
    22681 agatccagca gaagatgaat aaacaggcca gcctgaataa acgtgttcag gctcagcttg
    22741 gtacgctgtc caacctgtgg gaggcaatga cggggaccgc aactaacggc cttgcggcta
    22801 ttggcggcgc attttctggt gacgctaaga acatcacgca gtggctgggg gagttggggg
    22861 agaaattcac gaagtttgcg gatgaaaatc cccgggttat tcgcggcgtc gtcgggcttg
    22921 ctgccggtct tgcgattctg atactgggat tgatgggcgt gggcagtgcc atcagcattg
    22981 tcagcaggat catgtcgatg acgccgattg gcatgattgc gacggcgata gccatggctg
    23041 cgggattaat tatcactaac tgggatgttg tcggacctta tttcaagaag ctctgggaaa
    23101 ccattggtcc ttattttgag gctggctggg aactccttaa gaaagttttt gcctggtcgc
    23161 cgctggggat ggtgatcaat cactgggggc cggttgttaa gtggtttcag gatatgtggg
    23221 acaagctgaa gccaattatt gagtggttta ccgacagttc cggagacacg gtcgatgcca
    23281 ttaactctgc gcagtggggc gcgggtgctt atgatgctta tgggacggga ataccggcac
    23341 ggggatacac accttatccg gtggtggatc tggctcagtc aaacaacgcc tccggtgcca
    23401 taggcccgaa tcccttcatg attaataaag ctactgcgcc aaaagttgat ggtgagatca
    23461 aggtctcttt tgtgaattcg cctccgggta tgcgggttat ggaaacgcga tccagcggtt
    23521 ttgatgtcag ccatgatgtt ggctatacgc gctttggcag gtaatgaaaa attaatctgt
    23581 taatgagtcc cacaccggtg ggatttttta tgtacggagt ttatatgacg tggaaagaca
    23641 gacttcagga cgcgtcattt cgcggtgtgc cgtttaaggt tgaagaagaa agtgcgggaa
    23701 ccggtcgtcg tgtggaaacg cacgaatacc cgaaccgcga caaaccctat accgaagacc
    23761 tggggaaaat cactttccgc ccgtccatca cggcttatgt ggtgggcgat gactgctttg
    23821 accagcgcga tcgcctgatt gacgcgctga ataaacccgg tcccggcacg cttgtccatc
    23881 cgacatacgg cgagctgaaa gtctgtgttg acggagaagt tcgggtcagc acatcgaaga
    23941 gtgaagggcg tattgtccgc tttgacctga agtttgtcga agcgggagaa ctctcttacc
    24001 ccacatcagg tgcggcgacg gcgcagacgc tgatgtcatc ctgttctgca ctggatgact
    24061 gcatcagtga cagcttcagc ggtttcagta tcgatggtgt ggcggatttc gtgcagaacg
    24121 acgttatcgg taatgccagc ataatgctgg ggtatgtttc tgatgcgatg aaagtggtgg
    24181 attctgccgt atcggatgcc gccaggctgt tgcaggggga tatctcggta cttctgccgc
    24241 cgccatcgtc aggcaaaaat ttcgttgagc aggtgcagaa aatgtggcgt accgggaaac
    24301 gcctttatgg taacgccagc gacctggtca ccatgatcaa aacgctttcc gatgtcagcc
    24361 tcggcagcga tctgcaaccg cgcggcgtct ggaaaacgga cagtaaaacc accgccacgg
    24421 cgacgcagca gcgtaacgtg gttgccagca cccttcgtac gaccgcaatc agcgaagcgg
    24481 cgtatgccgt tacccgattg cctgcgccaa caacttccgc ggtgatgcag aatgccgcag
    24541 tggggcaggc aacaacaccc gcgcagagca cttgctggcc ttccgccacg catccggcac
    24601 tgaacaatgc accggcggtg aaaaacacgg ttgacctgcc gacgtgggaa gaactgactg
    24661 acattcgcga cacactgaat acggcaattg ataaggagtt gtcccgtaca accagcgatg
    24721 cgctgtttct ggcgctgcgc cgggtgaaag cagatctgaa tgcggatatc aacacgcgcc
    24781 ttgaacagtc tgcacggatc attcagcgca caccggatga ggttttaccc gcgctggtgc
    24841 tggcggagac ctggtttgat aacgcggcgc gtgacgcgga cattatccgg cgtaatgcca
    24901 ttacgcatcc cggctttgtg ccggtgatcc ctctgaaggt gccagtgcaa tgaacgacaa
    24961 tgtcacgcta cgggtaaatg gccgggagtg gaatggctgg acattggtgc gcatcggtgc
    25021 cggtattgaa cggctggcgc gggatttcag tgtggagatc acccgccagt ggccgggaga
    25081 tgagggtatt accacgcttc agccgcgcat taaaaacggt tcaaaagtgg aggtgctgat
    25141 tggtgatgag ctggtgatca ccggctgggt ggaggcgacg accgttcgtt acgatgcccg
    25201 ttcggtcatc accggtattg ccggacgcag tctgaccgct gacctgattg actgtgcagc
    25261 cgaaccgaca cagtttaacg gacgatcgct ggtacagatt gcgcaggcgc ttgctgcgcc
    25321 tttcggcatt gaggtggtga acagcggtgc gccgtcgggt gttattcctg atgttcagcc
    25381 tgatcacggt gaaacggtga ttgaggtaat caacaaaata ctcggtcagc agcaggcact
    25441 ggcttacgac gacccgcacg gcaggctggt gattggcggt attggctcaa cgcgggcaca
    25501 tactgcgctg gtactcgggg aaaacatcct ttcctgcgat acggagaaga gtatccggga
    25561 gcgattttct gtttaccagg tggcggggca gcgtgccgga aatgacgatg atttcggtga
    25621 ggccaccacc accgcgctgc gggcccgtac agaggacgca tttattgccc gttaccgtcc
    25681 gatgtatatc aggcagacag ggcaggctac gggggcaggc tgtattgcgc gtgctgactt
    25741 tgaagcccgg caacgggcgg cgcggacgga tgaaaccacc tatgtggtgc agggctggcg
    25801 acagggtaac ggtacgctgt ggcagcccaa ccagcgggtg attgtctttg atccggtctg
    25861 tggtttcgac aataccgaac tgcttgtttc ggaagtcacg tttactcagg accagaacgg
    25921 caccctgacg gaaatccgtg tcggcccgcc tgatgcttat ctgcctgaac ccgaagcccc
    25981 cggcgcgcgg aaaaagaaaa aagccagagt acaggaggac ccgttctgat gaggacgatt
    26041 gaagccatgc agcgacaact cctcggcctg attgggcggg cagtggtgaa aagcatcagc
    26101 gccgccacga aatgtcagac cgtggatgtg tccctgattg ccggtgaacc caaagccggg
    26161 attgaacatc ttgaacccta cggttttacc gcaagggcaa acagcggtgc ggaagcggtg
    26221 gtgttgtttc cggatggcga ccgttctcat gcggtggttg ttacggtgtc ggaccggcgc
    26281 taccgcctga aagggctgca gacgggtgag gtggctgtct atgacgatca ggggcagtct
    26341 gtgacgctga cccgggaggg gatcgtggtg gacggtgcag gtaaaacgat cacgtttcgc
    26401 aatgcgccta aggcacgttt tgaaatggac ctggaagtga caggacaggt gaaagacctg
    26461 tgcgactccg gcggcaccac catgtcagcg atgcggcttg cctataacgg gcatcgtcac
    26521 agagagaacg gtcagggcag taacaccgac aaacctgata aagcgatggg ggcatgatgg
    26581 aactgtggct gacggtgaac ggtaaacgca cctgcgccag cgcaccgctg gatccgctga
    26641 cccgcgccgt ggtgatttcc ctgtttacct ggcggcgggc ggagcctgat gacaacgccg
    26701 acgtcccgat gggatggtgg ggggatacct ggcctgcggt acagaatgac cgttacggct
    26761 cccgactgtg gctgcttcag cgcagcaaac tgaccaatca gctggtgcag acggtaaggg
    26821 ggtatatccg cgaatgcctg caatggatga ttgatgacgg cgtggtgtcc cgtattgatc
    26881 tggatatccg ccgcaccggg attaatgaac tgggtaacag tatcactctc tggcgtcgtg
    26941 acggaccggt aatgatttct tttgatgatc tgtggagtgc gataacgcat ggcggacagt
    27001 gaatttcagc gcccgacgct ggcagaaaat atcagtatgc tccgtaacga tttattcgtc
    27061 aggctggacg tcagcgacac gctccggcgc atggatgaag acgtgcgggc aaaggtgtat
    27121 gcggcggcgc tgcatacggt ctacgggtac atcgattatc tggcaatgaa tatgctgcct
    27181 gacctgtgcg atgagtcctg gctggcgcga catgctgcga tgaaacggtg tccgcgcaag
    27241 ggggccacgg ctgccagcgg gtatatgcgc ttggaaggtg tcagcgatgg cctgaaggtg
    27301 actgccggga gcgtgattca gcgcgatgac ctggttcagt acacggcaac tgccgatgca
    27361 accagctccg gtggtgtcct gcgtgtgccg atcacttgct caactacagg cgcggtcggt
    27421 aacgctgacg acggtacggc attaatcctg gtcacgccgg tgaatggtct gccgtcttcc
    27481 ggtgttgcag ataccctgac tggcggattc gatactgaag atctggaaac gtggcgcgcc
    27541 cgggtcattg agcggtatta ctggacgccg cagggcgggg ctgacgggga ctatgtcgtc
    27601 tgggctaaag aagtgcccgg cattacccgt gcatggacat accgtcactg gatgggaacg
    27661 ggaactgttg gtgtgatgat tgccagcagt gacctgatta accccattcc ggaagaatca
    27721 acggaaacgg cggcaagaca acatatcggg ccactggccc cggtggcagg ctctgatttg
    27781 tatgtgttca ggccggtggc acatacggtg gattttcata ttcgcgtgac gccggacaca
    27841 ccggaaatac gggctgccat caccgcggag ttgcgttcgt tcctgctgcg tgatggttat
    27901 ctgcagggag aactgaaggt atcgcgtatc agtgaggcga tttccggtgc gaacggggaa
    27961 tacagccatc agttgcttgc accggcagac aatatctcca ttgcaaaaaa tgaactggcg
    28021 gttctgggga cgatttcatg gacgtgacaa acgatgatta catccgtctg ttgtcggcac
    28081 tgctgccgcc cggtccggcg tggtcagcca gcgatccggc gattgccggt gcggcacagt
    28141 cattaacccg cgttcatcag cgtgcggatg ccctgatgcg ggagctggat ccgcgcacca
    28201 ccaccgaact gataaatcgc tgggagcgtc tgtgcggcct gccggatgaa tgtattcccg
    28261 caggaacaca gacccttcgc cagcgtcagc aacgactgga tgcgaaggtt aacttggcgg
    28321 gcggcatcaa tgaggatttt taccttgcac agcttgctgc cctgggcaga caagacgcca
    28381 ccatcacgcg atacgacaaa agcacgttca cctgctcatc ggcctgtact gacgcggtga
    28441 atgcgccgga atggcggtat tactggcagg tcaacatgcc agccgccacc aacagcacct
    28501 ggatgacatg tggcgatccc tgtgattccg cgctgcgtat ctggggcgac accgtcgtcg
    28561 aatgtgtgct taacaaactc tgcccttcgc atacctacgt aatttttaaa tatccggagt
    28621 aatccatgca tcgtatagac acgaaaaccg cgcagaagga taagttcggc gcgggtaaga
    28681 acggttttac ccgtggtaac ccccagactg gcacgcttgc caccgatctg gatgatgact
    28741 actttgacat gttgcaggag gagctttgca gcgtggtgga ggcatccggt gccagcctgg
    28801 agaaggggcg gcacgatcag ttgcttaccg cacttcgcgc gctgctgtta agccgcaaga
    28861 atccgtttgg cgatatcaaa tcggatggca cggtgaaaac ggctctcgaa aaccttggtt
    28921 tgggagaagc agctaaaagg aatgtaggta caggggccga a
//
LOCUS       DADLSB010000034        28461 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.034, whole genome shotgun sequence.
ACCESSION   DADLSB010000034 DADLSB010000000
VERSION     DADLSB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.034"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..241)
                     /locus_tag="JN331_001508"
     CDS             complement(<1..241)
                     /locus_tag="JN331_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY7259686.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPL"
     gene            551..2323
                     /gene="aspS"
                     /locus_tag="JN331_001509"
     CDS             551..2323
                     /gene="aspS"
                     /locus_tag="JN331_001509"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY7259687.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2441..2893
                     /gene="nudB"
                     /locus_tag="JN331_001510"
     CDS             2441..2893
                     /gene="nudB"
                     /locus_tag="JN331_001510"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY7259688.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAQGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2922..3662
                     /locus_tag="JN331_001511"
     CDS             2922..3662
                     /locus_tag="JN331_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7259689.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3697..4218
                     /gene="ruvC"
                     /locus_tag="JN331_001512"
     CDS             3697..4218
                     /gene="ruvC"
                     /locus_tag="JN331_001512"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY7259690.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQAGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4220..4822)
                     /locus_tag="JN331_001513"
     CDS             complement(4220..4822)
                     /locus_tag="JN331_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAY7259691.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSLQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4893..4958
                     /locus_tag="JN331_001514"
     CDS             4893..4958
                     /locus_tag="JN331_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259692.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5097..5708
                     /gene="ruvA"
                     /locus_tag="JN331_001515"
     CDS             5097..5708
                     /gene="ruvA"
                     /locus_tag="JN331_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY7259693.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            5717..6727
                     /gene="ruvB"
                     /locus_tag="JN331_001516"
     CDS             5717..6727
                     /gene="ruvB"
                     /locus_tag="JN331_001516"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY7259694.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6874..7659)
                     /gene="znuB"
                     /locus_tag="JN331_001517"
     CDS             complement(6874..7659)
                     /gene="znuB"
                     /locus_tag="JN331_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY7259695.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7656..8411)
                     /gene="znuC"
                     /locus_tag="JN331_001518"
     CDS             complement(7656..8411)
                     /gene="znuC"
                     /locus_tag="JN331_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY7259696.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8490..9422
                     /gene="znuA"
                     /locus_tag="JN331_001519"
     CDS             8490..9422
                     /gene="znuA"
                     /locus_tag="JN331_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY7259697.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9438..10760
                     /gene="mepM"
                     /locus_tag="JN331_001520"
     CDS             9438..10760
                     /gene="mepM"
                     /locus_tag="JN331_001520"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY7259698.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10880..11851
                     /gene="lpxM"
                     /locus_tag="JN331_001521"
                     /gene_synonym="msbB"
     CDS             10880..11851
                     /gene="lpxM"
                     /locus_tag="JN331_001521"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7259699.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11982..13424)
                     /gene="pyk"
                     /locus_tag="JN331_001522"
     CDS             complement(11982..13424)
                     /gene="pyk"
                     /locus_tag="JN331_001522"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY7259700.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13552..14421)
                     /locus_tag="JN331_001523"
     CDS             complement(13552..14421)
                     /locus_tag="JN331_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7259701.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14758..16233
                     /gene="zwf"
                     /locus_tag="JN331_001524"
     CDS             14758..16233
                     /gene="zwf"
                     /locus_tag="JN331_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY7259702.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16468..18279
                     /gene="edd"
                     /locus_tag="JN331_001525"
     CDS             16468..18279
                     /gene="edd"
                     /locus_tag="JN331_001525"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY7259703.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18316..18957
                     /locus_tag="JN331_001526"
     CDS             18316..18957
                     /locus_tag="JN331_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY7259704.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(19013..20191)
                     /gene="purT"
                     /locus_tag="JN331_001527"
     CDS             complement(19013..20191)
                     /gene="purT"
                     /locus_tag="JN331_001527"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY7259705.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20326..20616
                     /gene="yebG"
                     /locus_tag="JN331_001528"
     CDS             20326..20616
                     /gene="yebG"
                     /locus_tag="JN331_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HAY7259706.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            20646..21038
                     /gene="yebF"
                     /locus_tag="JN331_001529"
     CDS             20646..21038
                     /gene="yebF"
                     /locus_tag="JN331_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY7259707.1"
                     /translation="MLSPESLCYGEKHEKRGAFLGRLLVSACASVFAANNETSKSVTF
                     PKCEGLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVP
                     LTVRGKSADIHYQVSVDCKAGMAEYQRR"
     gene            21365..22024
                     /locus_tag="JN331_001530"
     CDS             21365..22024
                     /locus_tag="JN331_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY7259708.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQVIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22233..24293
                     /gene="ptrB"
                     /locus_tag="JN331_001531"
                     /pseudo
     CDS             22233..24293
                     /gene="ptrB"
                     /locus_tag="JN331_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
     gene            complement(24290..24952)
                     /gene="exoX"
                     /locus_tag="JN331_001532"
     CDS             complement(24290..24952)
                     /gene="exoX"
                     /locus_tag="JN331_001532"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY7259709.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24976..25632)
                     /locus_tag="JN331_001533"
     CDS             complement(24976..25632)
                     /locus_tag="JN331_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY7259710.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIPGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(25734..25964)
                     /locus_tag="JN331_001534"
     CDS             complement(25734..25964)
                     /locus_tag="JN331_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY7259711.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26103..26477
                     /gene="yobA"
                     /locus_tag="JN331_001535"
     CDS             26103..26477
                     /gene="yobA"
                     /locus_tag="JN331_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY7259712.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26481..27353
                     /gene="copD"
                     /locus_tag="JN331_001536"
     CDS             26481..27353
                     /gene="copD"
                     /locus_tag="JN331_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HAY7259713.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLMIRRLLTKRSL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            27366..27707
                     /locus_tag="JN331_001537"
     CDS             27366..27707
                     /locus_tag="JN331_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY7259714.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28100..>28461)
                     /locus_tag="JN331_001538"
     CDS             complement(28100..>28461)
                     /locus_tag="JN331_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7259715.1"
                     /translation="IAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGIRKNSPDG
                     SIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQRLLGHTSE
                     KTTKMYLDEREKTYLLL"
BASE COUNT         7200 a         7018 c         7392 g         6851 t
ORIGIN      
        1 taaggggtac gaataaagta acgggggcat caaccggctg ttttaatgct tcggcgtaat
       61 cggcagacca gccaactcgc accagaaaca cgggctgacc gctggcgcga aatttcgccg
      121 ccagcttccc ggcgcgatta actacctcat cggcagtatg tggaccaccg gcaaaaggta
      181 agatgccttc ttgtaaatcg atcaccacca acgcagtggt tttagcatta agttcaagca
      241 tgctgactcc tgaaaaaaca aaatcgtgcc tcacacctta atgcgattag ccgatggcgg
      301 aacgacaaat tttgttaatt tttatgagtt cgggcaacac ctttatagca aactggcgta
      361 gagtctgatt tcaggcccga tataaagtgg taacgaggcg ttccagcgcg ggccaggctc
      421 ttacggtgcg gcagaatttc cagtataata gccgcctttt ttcattcagt tgtgacatac
      481 agctaacgct gcgacttggt cacctgcgca gcagggggca tccgccggca gctaagttaa
      541 gggatatctc atgcgtacag aatattgtgg acagctccgt ttgtcccacg tggggcagca
      601 ggtgactctg tgtggttggg tcaaccgtcg tcgtgatctt ggtagcctga tcttcatcga
      661 tatgcgcgac cgcgaaggta tcgtgcaggt atttttcgat ccggatcgtg cggacgcgtt
      721 aaagctggcc tctgaactgc gtaatgagtt ctgcattcag gttacgggca ccgtacgtgc
      781 gcgtgacgaa aaaaatatta accgcgatat ggcgaccggc gaaattgaag tgctggcgtc
      841 ctcgctgact atcatcaacc gcgcagatgt tctgccgctt gactctaacc acgtcaacac
      901 cgaagaagcg cgtctgaaat accgctacct cgacctgcgt cgcccggaaa tggcgcagcg
      961 cctgaaaacc cgcgctaaaa tcaccagcct ggtgcgccgt tttatggatg accacggctt
     1021 cctcgacatc gaaactccga tgctgaccaa agccacgccg gaaggcgcgc gtgactacct
     1081 ggtgccttct cgtgtgcaca aaggtaaatt ctacgcactg ccgcaatccc cacagttgtt
     1141 caaacagctg ctgatgatgt ccggttttga ccgttactat cagatcgtta aatgcttccg
     1201 tgacgaagac ctgcgcgctg atcgtcagcc tgaatttact cagatcgatg tggaaacttc
     1261 tttcatgacc gcgccgcaag tgcgtgaagt gatggaagcg ctggtgcgtc atttgtggct
     1321 ggaagtgaag ggcgtggatc tgggcgattt cccggtaatg acctttgcgg aagcagaacg
     1381 ccgttatggt tctgataaac cggatctgcg taacccgatg gaactgaccg acgttgccga
     1441 tctgctgaaa tctgttgagt ttgcagtatt tgcggggccg gcgaacgatc cgaaaggccg
     1501 tgttgccgct ctacgtgttc cgggtggcgc atcgctgacc cgtaagcaga tcgacgaata
     1561 cggtaacttc gttaaaatct acggcgcgaa aggtctggct tacatcaaag ttaacgaacg
     1621 cgcgaaaggt ctggaaggta tcaacagccc ggtagcgaag ttccttaatg cagaaatcat
     1681 cgaagccatc ctggatcgta ctgccgcgca agatggcgat atgattttct tcggtgccga
     1741 caacaagaaa attgttgccg acgggatggg cgcactgcgc ctgaaagtgg gtaaagacct
     1801 tggtctgacc gacgaaagca aatgggcacc gctgtgggtt atcgacttcc cgatgtttga
     1861 agacgacggt gaaggcggcc tgacggcaat gcaccatccg ttcacctcac cgaaagacat
     1921 gactgctgca gaactgaaag ctgcaccgga aaatgcggtg gcgaacgctt acgatatggt
     1981 catcaatggt tacgaagtgg gcggtggttc agtacgtatc cataatggtg atatgcagca
     2041 gacggtgttt ggtattctgg gtatcaacga agaggaacag cgcgagaaat tcggcttcct
     2101 gctcgacgcc ctgaaatacg gtactccgcc gcacgcaggt ctggcattcg gtcttgaccg
     2161 tctgaccatg ctgctgactg gcaccgacaa tatccgtgac gttatcgcct tcccgaaaac
     2221 cacggcggca gcgtgtctga tgactgaagc accgagcttt gctaacccga ctgcactggc
     2281 tgagctgagc attcaggttg tgaagaaggc tgagaataac tgatatgact caaatacacg
     2341 aaatcattcg cgttgcatca aggcggcaac tgagtgaact cccatgagca tagataacta
     2401 tgtgaatggg atgagcgaag gcagtcaacg aagaggcagc gtgaaggata aagtgtataa
     2461 gcgtcccgtt tcgatcttag tggtcatcta tgcacaagat acgaaacggg tgctgatgtt
     2521 gcagcggcgt gacgatcccg atttctggca gtcggtaacc ggcagcgtgg aagagggtga
     2581 aaccgcgccg caagctgcca tgcgcgaagt aaaggaagag gtcaccattg atgttgtcgc
     2641 tgaacaactg accttaattg actgtcagcg cacggtagag tttgaaattt tttcacattt
     2701 acgtcatcgc tatgcgcagg gcgtgacgcg taatacggaa tcatggttct gtcttgcgct
     2761 tccgcacgag cggcagatcg ttttcactga acatctggct tacaagtggc ttgatgcgcc
     2821 tgcggcggcg gcgctcacta agtcctggag caaccggcag gcgattgaac agtttgtaat
     2881 taacgctgcc tgaacaggca gggctatctg gagatatttt tatggcaggt catagtaaat
     2941 gggccaacac cagacatcgt aaagctgcgc aggatgctaa gcgcggtaaa atcttcacta
     3001 aaatcattcg tgagctggta accgcggcta agctgggcgg tggcgatccg gacgctaacc
     3061 cgcgtctgcg tgcggcggtg gataaagcac tgtctaacaa catgacccgt gacacactga
     3121 accgcgcaat tgcacgtggt gtgggcggtg atgatgatgc aaacatggaa accatcatct
     3181 acgaaggtta cggtcctggc ggcacggcaa tcatgattga atgtctgtct gacaaccgca
     3241 accgtaccgt tgctgaagtg cgtcacgcat tcagcaaatg tggcggtaac ctcggcactg
     3301 acggttccgt agcttatctg ttcagcaaaa aaggcgtgat ctccttcgag aaaggcgacg
     3361 aagacaccat catggaagca gctctggaag caggtgctga agacgttgtg acatatgatg
     3421 acggcgcgat tgatgtctac actgcatggg aagaaatggg taaagtgcgc gacgctctgg
     3481 aagcggcagg tctgaaagca gacagcgcgg aagtttccat gatcccgtcc accaaagctg
     3541 atatggatgc agaaaccgcg ccgaaactga tgcgtctgat cgatatgctg gaagattgcg
     3601 acgacgtgca ggaagtttac cataacggtg aaatctctga tgaggttgca gcgactctct
     3661 gatgatgcct gctaaacagc aaaacggaga cgcgtgatgg ctattattct cggcattgac
     3721 ccgggttcgc gcgtgaccgg ctacggcgtc atccgccagg caggtaggca actgtcctac
     3781 ctgggtagcg gatgcatacg caccaaagtg gatgatttac cgtctcgtct gaagctcatc
     3841 tatgcgggcg tgacggaaat catcacccag ttccagcctg attatttcgc cattgaacaa
     3901 gtctttatgg cgaagaacgc tgactcagcc ctgaaactgg gccaggcgcg cggcgtggcg
     3961 attgttgcgg cggtgaatca ggaattgccg gtatttgaat acgcggcacg tcaggtaaag
     4021 caaacggtgg taggtatggg tagtgccgaa aaaagccagg tgcagcatat ggtccgcacc
     4081 ttgctgaaac tgcccgctaa tccacaggcg gatgccgccg atgcgctggc gattgccatc
     4141 acccactgcc acgttagtca gaatgcgatg cagatgagcg aatcgcggct gaacctggcg
     4201 agagggcgac tgcgttaagt tataccgcct cggtcagttc cggctgaggc gtttcaactc
     4261 cctccgcgtg aatcaacacc aaacccggat gatgccacaa actggctggc gtgacggttt
     4321 tacgctccca cggaatagaa cctaagaacc agaatttcca gaaagtgagt tttgcattcg
     4381 gattgctatt taacagttgt ccaaacgtct ctatttcacc caccggaata catagcgcct
     4441 ctttataaat atcaaagaca aattttgaac agaactggag cgaagattcg tatttaaaac
     4501 ccgtgtggta aagtttgcgt agccgggagg gaacctgttc aacaattcgt tgtttttgtt
     4561 gttctgtcag cccagcgtct aatcgcttta tagcatagcg ttgattagac gaacgtttaa
     4621 taaaacgaga tagcgtggtg atggttgaaa ggggaacccg gctttctgca accaggaagt
     4681 cttcaccgtt atgaccgata atgatcccga cgtgattact ccagcaattt gatgcagctg
     4741 atatttgacc aaataaggca gcacctatac atgtaaagac gatatctcca atttcatatt
     4801 cagcagggta attaatattc acgttgtcat tccattgaaa tagatacgaa tatacgtaat
     4861 agcataaaaa aattattttt tcaggagtcg caatgtatat ttttattacg catttcttca
     4921 ctgaatatgt aatattcaaa tatttgcttc caatataacc tgttgaataa attatactgt
     4981 gccatttttc agatcatcgg gacacctcgc aagttttctt catccttcgc tggatatcta
     5041 tccagcattt ttttatcata cagcattatc tttgattcat tacgcaggag cgtcatgtga
     5101 taggcagact cagaggcatc atcattgaaa aacaaccccc gctggtgtta attgaagtgg
     5161 gcggcgtagg ctatgaagtg catatgccga tgacctgttt ttatgaactc cctgaagcgg
     5221 gtcaggaagc gatcgttttc acccactttg tggtgcgtga agacgcgcaa ctgctgtacg
     5281 gttttaacaa taaacaagaa cgcacgttgt tcaaagagtt gatcaaaacc aacggcgtcg
     5341 gcccgaagtt ggccctggcg atcctctccg gaatgtcagc gcagcagttc gttaatgccg
     5401 ttgagcgtga agaagtgggg gcactggtga aactgccggg tattggcaaa aaaaccgccg
     5461 aacgcttgat tgttgaaatg aaagaccgat ttaaaggttt gcatggcgat ctctttacgc
     5521 cagccgccga cctggtactc acgtctcctg ccagcccggc gaccgacgat gctgaacaag
     5581 aagcagttgc cgcgctggtg gcgctaggct ataaaccaca agaagcaagc cgcatggtga
     5641 gcaaaatcgc tcgccctgac gccagcagtg aaacattaat tcgcgaagcc ctacgcgccg
     5701 cgttatgagg taaaggatga ttgaagcaga ccgtctgatt tctgccggta ccactttgcc
     5761 ggaagatgta gcagatcgcg ccattcgccc taaattactg gaagagtatg ttggtcagcc
     5821 gcaggttcgt tcacagatgg agattttcat caaagcagcg aaactgcgcg gcgatgccct
     5881 cgatcatttg ttgatttttg gtcctccggg gttgggtaaa actacgcttg ccaacattgt
     5941 cgccaatgaa atgggcgtta atttacgcac gacttctggt ccggtgctgg aaaaggcggg
     6001 cgatttggct gcgatgctca ctaaccttga accgcatgac gtgctgttta ttgatgagat
     6061 ccaccgttta tcgccagttg ttgaagaagt gctgtacccg gcaatggaag actaccaact
     6121 ggatatcatg attggtgaag gtccggcggc acgctccatt aaaattgatt tgccgccgtt
     6181 taccctgatt ggcgcaacca cgcgcgcagg ttcgctgaca tcaccgttgc gcgaccgttt
     6241 tggtattgtg caacgtctgg agttttatca ggtgccggat ctgcaatata tcgtcagtcg
     6301 cagtgcacgc tttatggggc ttgagatgag tgatgacggt gcgctggaag tagctcgtcg
     6361 cgctcgcggt acgccacgta ttgccaaccg tctgctgcgt cgagtgcgtg atttcgccga
     6421 agtgaagcac gatggcacca tctcggcaga tatcgctgct caggcgctgg atatgttgaa
     6481 tgtcgatgct gaaggtttcg attatatgga ccgcaaattg ttgctggcgg taatcgataa
     6541 gttctttggt gggccggtag gtctggataa cctggcggca gccattggcg aagaacgtga
     6601 aaccattgaa gatgtactgg aaccttattt gattcagcaa ggctttttgc agcgtacacc
     6661 gcgcgggcgt atggcgacga cgcgggcgtg gaatcacttt ggcataacgc cgccagaaat
     6721 gccgtaagtc ggattggtcg ctcgcgccgc atccgacact ggcagtgtgt ctgatgcgac
     6781 actgttcgcg tcttatcagg ccagccagag atagcgaacc gtaggtcgga taaggcgctc
     6841 gcgccgcatc cgacaaatgt gttcagcgat agattagctg gcctgctttt tcatcatact
     6901 gataataaat agcagtgccg cacatagcac caccgacgga cccgccggcg tatcgtaaaa
     6961 cgcggaaaag gttaaaccgc cagttactgc caccatcccc accaaaacag cgacaccagc
     7021 catctgttcc ggcgtacggg caaagcgacg cgcagtagct gcaggaataa tcagcaacga
     7081 agtaataatc aacgcaccga caaatttcat cgctacacca atcgtcaatg ccgtcaccag
     7141 catcaacaac aatttcactt gctgtaattt cacaccatca acaaacgcca gatccgggct
     7201 aatcgtcatc gacagcaaat tgcgccattg ccagaacaaa atagccacca cgatgaccac
     7261 gccaatcgca atagagatga gatcttctgg cgtcactgcc agtaaatcac cgaacaggta
     7321 agccatcaaa tcaacacgaa tgttagacat cagactaacg accactaggc ccagcgacag
     7381 ggcactgtgc gccataatcc ctaataacgt gtcgatcgcc agctgtggac gcttctccag
     7441 ccataccaga ccgcccgcca gcagcagcgt aacggcaatc accgcataga atggattcac
     7501 gtccagcaac aaaccaaacg cgacaccaag taatgaggca tgagccagcg tatcaccgaa
     7561 ataagacata cgacgccaga ctacaaacga acccagcgga cccgcggcac aggcgagcat
     7621 gatcccggct aaccaaccgg gaaataataa ttcaatcatg agcgatcatt tccccgacgc
     7681 aaaacaattc gtccctgtaa atcgtgacga tgattatgat gatggcgata gatacccagt
     7741 tgttcagcac cacgaggacc aaacattgaa ataaactccg gatgcaggga aacaacttcc
     7801 ggtgtgccgg aacaacaaat gtggtgattc aggcacagca cttcatcggt ttttgccatc
     7861 accagatgca gatcgtgaga aaccattaaa acgccacaat ccagttcacg acgcaactgg
     7921 tcaataaggt catataacgc cacttggcca tttacatcca cgccttgagt gggttcatcc
     7981 agcactaata attgcgggca atttaacaat gctcgcgcta atagtacacg ctgcgtttcg
     8041 ccaccagaga gcttttgcat cggtgcgtta atcaggtgcc cggcctggac acgtttcagt
     8101 gcaggcaaaa tatcttcttt atgtgtacca gggcgtaagc gtaaaaaacg gtttacggtc
     8161 agtggcaacg tggtgtcgag atacagcttc tgcggtacat agccgatgcg cagttttccg
     8221 ttgcgcttga taaccccttc atcgggtgtt accagcccga gcactacccg taccagtgtc
     8281 gacttacctg cgccatttgg cccaagtaaa gtcaaaattt ttccaggttt aagttccagc
     8341 gacacatcag agaggacgcg gcgttggcca aaagaaaccg agacattttc cagggaaacc
     8401 agacttgtca tgttaatttt agtcttgcag tagtcatgaa atgttataat atcacacttc
     8461 tcatattcat tacgatgatt ggtcgcatta tgttacataa aaaaacgctt cttttcgcag
     8521 cattatccgc cgctctctgg ggaggtgcaa cacaggccgc agatgccgcc gttgtcgctt
     8581 cgcttaaacc cgttgggttc atcgcttctg ccattgctga tggtgtaaca gaaacggagg
     8641 ttttactgcc tgacggcgct tcagaacatg attattcact gcgtccatcg gatgtaaaac
     8701 gcttacagaa cgcggactta gtcgtttggg ttggcccgga gatggaagcg tttatgcaaa
     8761 aaccggtaag caaattacca gaagcgaagc aggtaacgat tgcgcagctt gaagatgtga
     8821 aaccgctact gatgaaaagt attcacggcg atgatgatga tcacgaccac gcggaaaaaa
     8881 gtgacgaaga tcaccatcac ggcgatttca acatgcatct ttggctttcc ccagagatag
     8941 cgcgggctac agcggttgca atccatggaa aattagtgga acttatgccg caaagtcgag
     9001 ccaaacttga cgccaacctg aaggattttg aggcacaatt ggcctcaacc gaaacgcagg
     9061 ttggtaacga gctcgcgccg ctcaagggga aaggttattt cgtttttcac gatgcttacg
     9121 gctatttcga aaaacagttc ggactgacac cgcttggtca ttttaccgtt aaccctgaga
     9181 ttcaacctgg cgcgcagcgt ttacatgaaa taagaacaca gttggttgag caaaaagcaa
     9241 cctgcgtttt tgctgagcca cagttcaggc cagcggtcgt tgagagcgtc gcacgaggga
     9301 catccgttcg tatgggaacg ttggatcccc tggggacgaa tatcaaactg ggtaaaacaa
     9361 gctattcaga attcctgagt caattagcca accagtatgc gagctgcctg aaaggagatt
     9421 aatgaggaag tgattacgtg caacagatag cccgctctgt cgccctggcg tttaataatt
     9481 taccgcgacc acaccgcgtt atgttggggt cgctcaccgt tcttactctg gccgtcgctg
     9541 tctggcggcc ctatgtttat caccgtgatg ccacgccaat tgtcaaaacc attgagctgg
     9601 aacagaacga aattcgttcg ctcttacctg aagccagtga gccgattgat caagctgcac
     9661 aagaagatga agccattccc caggacgaac tggatgacaa aatcgccggt gaagcgggcg
     9721 tgcatgaata tgttgtttcc actggcgata cgctaagcag cattctcaat cagtatggta
     9781 ttgatatggg tgatatcacc caactggctg ctgccgacaa agaattgcgt aacctgaaaa
     9841 tcggtcaaca actctcctgg acattaaccg cggacggcga actgcagcgc ctcacctggg
     9901 aagtgtctcg tcgtgaaacc cgaacctatg accgtactgc cgctaacggt tttaaaatga
     9961 ccagcgaaat gcagcaagga gagtgggtta acaatctgct gaaaggtacc gtcggaggga
    10021 gctttgttgc cagcgccaga aacgccggtt taaccagcgc cgaagtgagc gcagtgatta
    10081 aagccatgca gtggcaaatg gatttccgca aactgaaaaa aggcgatgaa tttgcggtat
    10141 taatgtctcg agaaatgctt gatggtaaac gtgagcaaag ccagctgctg ggcgtacgtt
    10201 tgcgttcaga aggtaaagat tattacgcaa tccgcgctga ggatggcaaa ttctacgatc
    10261 gtaacggtac tggtctggcg aaaggattct tgcgattccc gacggcgaaa cagttccgta
    10321 tctcgtctaa ctttaacccg cgtcgaacta atccggtgac aggtcgcgtt gcaccacaca
    10381 gaggtgttga tttcgccatg ccacaaggta cgccagtgct ttcagtgggt gacggtgaag
    10441 tggtggttgc caaacgcagt ggcgcagcag gttattatgt ggctattcgt catggtcgca
    10501 gctacaccac gcgttatatg cacttgcgca agattctggt gaaaccggga cagaaggtga
    10561 aacgtggcga ccgtatcgcg ctttccggta ataccggacg ttcaactggg ccgcatctgc
    10621 actatgaagt atggataaac cagcaggccg taaacccgct gacggcaaaa ctgccgcgta
    10681 ccgaagggct gaccggctcc gatcgtcgcg aattcctggc gcaggccaaa gagattgtgc
    10741 cgcagctacg gtttgattaa ttaacatcca ttcgcagccg gtacgcagtc agtaccggct
    10801 ttttttattt ggtgcggggc aagttgcgcc gctacactat caccagattg atttttgcct
    10861 tatccgaaac tggaaaagca tggaaacgaa aaaaaataat agcgaataca ttcctgagtt
    10921 tgataaatcc tttcgccacc cgcgctactg gggagcatgg ctgggtgtag cagcgatggc
    10981 gggtatcgct ttaacgccgc caaagttccg tgatcccatt ctggcacggc tgggacgttt
    11041 tgccggacga ctgggaaaaa gctcacgccg tcgtgcgtta atcaatctgt cgctctgctt
    11101 tccagaacgt agtgaagctg aacgcgaagc gattgttgat gagatgtttg ccaccgcgcc
    11161 gcaagcgatg gcaatgatgg ctgagttggc aatacgcggg ccggagaaaa ttcagccgcg
    11221 cgttggctgg caagggctgg agatcatcga agagatgcgg cgtaataacg agaaagttat
    11281 ctttctggtg ccgcacggtt gggccgtcga tattcctgcc atgctgatgg cctcgcaagg
    11341 gcagaaaatg gcagcgatgt tccataatca gggcaacccg gtttttgatt atgtctggaa
    11401 cacggtgcgt cgtcgctttg gcggtcgtct gcatgcgaga aatgacggta ttaaaccatt
    11461 catccagtcg gtacgtcagg ggtactgggg atattattta cccgatcagg atcatggccc
    11521 agagcacagc gaatttgtgg atttctttgc cacctataaa gcgacgttgc ccgcgattgg
    11581 tcgtttgatg aaagtatgcc gtgcgcgcgt tgtaccgctg tttccgattt atgatggcaa
    11641 gacgcatcgt ctgacgattc aggtgcgccc accgatggat gatctgttag aggcggatga
    11701 tcatacgatt gagcggcgga tgaatgaaga agtcgagatt tttgttggtc cgcgaccaga
    11761 acaatacacc tggatactaa aattgctgaa aactcgcaaa ccgggcgaaa tccagccgta
    11821 taagcgcaaa gatctttatc ccatcaaata aaaaagcctc tcgcgaggag aggccttcgc
    11881 ctgatgataa gttcaagttt gcttcagaat attcgaaatc tgttgaacta tcattgaact
    11941 ataggccgga tgtggcgttt tcgccgcatc cggcaacgta cttactctac cgttaaaata
    12001 cgcgtggtat tagtagaacc cacggtactc atcacgtcgc cctgggtgac aatcaccagg
    12061 tcaccagaca tcaagtaacc tttatcgcgc agcagattaa ccgcttcgct ggcagctgct
    12121 acgccgtcat tagcgctatc aaagtgcacc ggcgtaacgc cacgatagag agcagtcagg
    12181 ttcagcgtac gttcatggcg cgacatggcg aaaattggta gaccagagct gatacgggag
    12241 gtcatcagcg cggtacgacc cgattcggtc atggtaatga tcgccgtaac gcccttcagg
    12301 tggttagctg cgtacattgc cgacatggca atagcttctt ccacattgtc gaactgaacg
    12361 tccagacggt gtttagaaac gttgatgctc gggatttttt ccgcacccag gcaaacgcgc
    12421 gccatggctg caacagtttc tgacggatac tgcccagcgg cagtttctgc agacagcatc
    12481 acagcgtcag taccatccag aacggcgttt gctacgtcca tgacttctgc acgcgtcggc
    12541 atcgggttag taatcattga ctccatcatc tgggtcgccg tgattaccgc tcggtttagc
    12601 tgacgcgcac gacggatcaa cgctttctga atgccgacca gttccgggtc gccaatttcc
    12661 acaccgaggt cgccacgtgc aaccattacc acgtcagagg cgaggatgat gtcatccatt
    12721 gcatcctggc tgcaaacggc ttccgcacgt tcaaccttgg caacaatttt cgcatcacat
    12781 cctgcatcgc gtgccaggcg acgggcatag ttcagatctt cgccacagcg tgggaaggag
    12841 acagccaggt aatctacgcc aatcaacgcc gcagtcttaa tgtctgcttt gtctttttcg
    12901 gtcagcgctt cagccgacaa accgccgcca agtttgttga tacctttatt gttggagagg
    12961 ggaccaccga cggtgacttc ggtgaacact ttcatgccct gaacttccag tacttttaac
    13021 tggacgcgac catcgtccag cagcaggatg tcaccaggca cgacgtcagc aggcaggcct
    13081 ttgtagtcga taccgacttt ttctttgtcg ccttcacctt tacctaggtt ggcgtcgagc
    13141 aggaatttat ccccaatatt gaggaaaact ttgccttctt taaaggtgga tacacggatt
    13201 ttgggcccct ggaggtcacc cagaatagcc acatgacgcc ccagttttgc ggcaatctca
    13261 cgaactttat ccgcgcgcat tttgtgatct tcaggcgagc cgtgagaaaa gttcatacgt
    13321 acaacgttgg cacccgccgc gataactttt tcaagattat tatcgcgatc tgttgctggg
    13381 cctaacgtgg taacgatttt tgttctgcga agccttttgg acatgtaata ctccgttgac
    13441 tgaaacaacc aggtgttgct tgaacatgaa atccgaatga aataacgccg cgatggtgat
    13501 aaattaatct cattcggatg tcgtataaga ttaggacagt gacagtcgct tttagcgatc
    13561 gtcacttaaa ttaagtaact gcttatcaaa acgcgattcc ttcagcgctt ccttgacccg
    13621 cttcaagtta tctctgaatt ttgcaccgcg tcgcaaagta aatcctgtcg ccagcacatc
    13681 tatcacggtc agctgtgcaa gtcgagaaac catgggcata taaatgtcag tatcttccgg
    13741 tacgtcgagg gtaattgcca gcgttgcttc ccgggcgagc ggggtacccg cagaggtgag
    13801 ggcaatcacc atggcgtcgt tttcgcgtgc cagctgcgcc agctcgacca gattttttgt
    13861 tcttcctgtg tgagaaatca gcaccaccac gtctccgtcg ctacaattca tacaactcat
    13921 gcgttgcagc acgatatcat cggagtacac caccggaaca ttaaaacgaa agaacttatt
    13981 catcgcatcg tgggcaacgg cggcagaaga gcctaatccg aaaaaggcga ttttttttgc
    14041 ctgagtgagc aagtcgacgg cgcggttgat ggcagattta tccagtgaat gacggacatg
    14101 atcgagcgtt gccattgcgg actcaaatat tttccccgtg tatgattcaa cgctgtcatc
    14161 ttcattgacg ttgcgattaa catagggagt gccattcgcc agactttgtg ccagatgaag
    14221 tttaaaatca ggaaaaccgc gcgtgtccat gctgcgacag aaacgattca ccgtcggttc
    14281 gctaacattg gcttccagcg ccagagcagc aatactcgaa tggatcgcgt tatcgggcga
    14341 agccagaatg acctcggcaa ctttgcgctc tgatttgctc aaatgttcca gctgagactg
    14401 gattttttcc agcatattca tgatgtaaag agactcacgg gtaatgacga tttccgcact
    14461 gaaagaaatc gaaatgcagt tttgtcagat attacgcctg tgtgccgtgt taatgacaaa
    14521 agcagataaa aaagttgtta tttttttcat aacatgatca gtgtcagatt tttaccgaat
    14581 ggaaaacgat gattttttta tcagttttgc cgcactttgc gcgcttttcc cgtaatcgca
    14641 cgggtggata agcgtttaca gttttcgcaa gctcgtaaaa gcagtacagt gcaccgtaag
    14701 aaaattacaa gtataccctg gcttaagtac cgggttagtt aacttaagga gaatgacatg
    14761 gcggtaacgc aaacagccca ggcctgtgac ctggtcattt tcggcgcgaa aggcgacctt
    14821 gcgcgtcgta aattgctgcc ttccctgtat caactggaaa aagccggtca gctcaacccg
    14881 gacacccgga ttatcggcgt agggcgtgct gactgggata aagcggcata taccaaagtt
    14941 gtccgcgagg cgctcgaaac tttcatgaaa gaaaccattg atgaaggttt atgggacacc
    15001 ctgagtgcac gtctggattt ttgtaatctc gatgtcaatg acactgctgc attcaaccgt
    15061 ctcggcgcga tgctggatca aaaaaatcgt atcaccatta actactttgc catgccgccc
    15121 agcacttttg gcgcaatttg caaagggctt ggcgaggcaa aactgaatgc taaaccggca
    15181 cgcgtagtca tggagaaacc gctggggacg tcgctggcga cctcgcagga aatcaatgat
    15241 caggttggcg aatacttcga ggagtgccag gtttaccgta tcgaccacta tcttggtaaa
    15301 gaaacggtgc tgaacctgtt ggcgctgcgt tttgctaact ccctgtttgt gaataactgg
    15361 gacaatcgca ccattgatca tgttgagatt accgtggcag aagaagtggg gatcgaaggg
    15421 cgctggggct attttgataa agccggtcag atgcgtgaca tgattcagaa ccacctgcta
    15481 caaattctct gcatgattgc gatgtctccg ccatctgacc tgagcgcaga cagcatccgc
    15541 gatgaaaaag tgaaagtact gaagtccctg cgccgcatcg accgctccaa cgtacgcgaa
    15601 aaaaccgtac gcgggcaata tactgcgggc tttgcccagg gcaaaaaagt gccgggatat
    15661 ctggaagaag agggcgcgaa caaaagcagc aatacagaaa ccttcgtggc gatccgcgtc
    15721 gacattgata actggcgctg ggccggtgtg ccattctacc tgcgtactgg taaacgtctg
    15781 ccgaccaaat gttctgaagt cgtggtctat ttcaaaacac ctgaactgaa tctgtttaaa
    15841 gagtcgtggc aggatctgcc gcagaataaa ctgactatcc gtctacaacc tgatgaaggc
    15901 gtggatatcc aggtactgaa taaagttcct ggccttgacc acaaacataa cctgcaaatc
    15961 accaagctgg atctgagcta ttcagaaacc tttaatcaga cgcatctggc ggatgcctat
    16021 gaacgtttgc tgctggaaac catgcgtggt attcaggcac tgtttgtacg tcgcgacgaa
    16081 gtggaagaag cctggaaatg ggtagactcc attactgagg cgtgggcgat ggacaatgat
    16141 gcgccgaaac cgtatcaggc cggaacctgg ggacccgttg cctcggtggc gatgattacc
    16201 cgtgatggtc gttcctggaa tgagtttgag taatatctgt gcttatcctt tatggttatt
    16261 ttaccggtaa catgatcttg cacagattgt agaacaattt ttacactttc aggcctcgtg
    16321 cggattcacc cacgaggctt tttttattac actgactgaa acgtttttgc cctatgagct
    16381 ccggttacag gcgtttcact cataaatcct ctgaatgaaa cgcgttgtga atcatcctgc
    16441 tctgacaact caatttcagg agcctttatg aatccacaat tgttacgcgt aacaaatcga
    16501 atcattgaac gttcgcgcga gactcgctct gcttatctcg cccggataga acaagcgaaa
    16561 acgtcgaccg ttcatcgttc gcagttggca tgcggtaacc tggcacacgg tttcgctgcc
    16621 tgccagccag aagacaaagc ctctttgaaa agcatgttgc gtaacaatat cgccatcatc
    16681 acctcctata acgacatgct ctccgcgcac cagccttatg aacactatcc agaaatcatt
    16741 cgtaaagccc tgcatgaagc gaatgcggtt ggtcaggttg cgggcggtgt tccggcgatg
    16801 tgtgatggtg tcacccaggg gcaggatgga atggaattgt cgctgctaag ccgcgaagtg
    16861 atagcgatgt ctgcggcggt ggggctgtcc cataacatgt ttgatggtgc tctgttcctc
    16921 ggtgtgtgcg acaagattgt cccgggtctg acgatggcag ccctgtcgtt tggtcatttg
    16981 cctgcggtgt ttgtgccgtc tggaccgatg gcaagcggtt tgccaaataa agaaaaagtg
    17041 cgtattcgcc agctttatgc cgaaggtaaa gtggaccgca tggccttact ggagtcagaa
    17101 gccgcgtctt accatgcgcc gggaacatgt actttctacg gtactgccaa cactaaccag
    17161 atggtggtgg aatttatggg gatgcagttg ccaggctctt cttttgttca tccggattct
    17221 ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg ttacacgcat gaccggtaat
    17281 ggtaatgaat ggatgccgat cggtaagatg atcgatgaga aagtggtggt gaacggtatc
    17341 gttgcactgc tggcgaccgg tggttccact aaccacacca tgcacctggt ggcgatggcg
    17401 cgcgcggccg gtattcagat taactgggat gacttctctg acctttctga tgttgtaccg
    17461 ctgatggcac gtctctaccc gaacggtccg gccgatatta accacttcca ggcggcaggt
    17521 ggcgtaccgg ttctggtgcg tgaactgctc aaagctggcc tgctgcatga agatgtcaat
    17581 acggtggcag gctttggtct gtctcgttat acccttgaac catggctgaa taatggtgaa
    17641 ctggactggc gggaaggggc ggaaaaatca ctcgacagca atgtgatcgc ttccttcgaa
    17701 caacctttct ctcatcatgg tgggacaaaa gtgttaagcg gtaacctggg ccgtgcggtt
    17761 atgaaaacct ctgccgtgcc ggttgagaac caggtgattg aagcgccagc ggttgttttt
    17821 gaaagccagc atgacgttat gccggccttt gaagcgggtt tgctggaccg cgattgtgtc
    17881 gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc cagaattaca taaactcatg
    17941 ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg cgttagttac cgatggacga
    18001 ctctccggcg cttcaggtaa agtgccgtca gctatccacg taacaccaga agcctacgat
    18061 ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc gtgtgaatgg acagacaggc
    18121 gaactgacgc tgctggtaga cgaagcggaa ctggctgctc gcgaaccgca cattcctgac
    18181 ctgagcgcgt cacgcgtggg aacaggacgt gaattattca gcgccttgcg tgaaaaactg
    18241 tccggtgccg aacagggcgc aacctgtatc actttttaag acgacaaatt tgtaatcagg
    18301 cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag aatcaatcct gaccaccggc
    18361 ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg cggtgccgat ggcaaaagcg
    18421 ttggttgctg gtggggtgcg cgttctggaa gtgactctgc gtaccgagtg tgcagttgac
    18481 gctatccgtg ctatcgccaa agaagtgcct gaagcgattg tgggtgccgg tacggtgctg
    18541 aatccacagc agctggcaga agtcactgaa gcgggtgcac agttcgcaat tagcccgggt
    18601 ctgaccgagc cgctgctgaa agctgctacc gaagggacta ttcctctgat tccggggatc
    18661 agcactgttt ccgaactgat gctgggtatg gactacggtt tgaaagagtt caaattcttc
    18721 ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga tcgcgggtcc gttctcccag
    18781 gtccgtttct gcccgacggg tggtatttct ccggctaact accgtgacta cctggcactg
    18841 aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg cagatgcgct ggaagcgggc
    18901 gattacgacc gcattactaa gctggcgcgt gaagctgtag aaggcgctaa gctgtaaaag
    18961 tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt tttttgccgg gtttaaccct
    19021 gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat ggcgtcaaca acactctctg
    19081 aagtagccag taccaccccc agacgacggc tgccatcaat ttccggctta ccaaataaac
    19141 gaatctgcaa atctgcgcct acggcattct gcacattatc aaacgtgaca ttctgactgg
    19201 tcagttgtgg cagaataacg gcagaagctg caggaccata ctgacggatc ccgccaacag
    19261 gaagtccgag gaaggcacgt acatgcaggg caaactctga gagatcttga gaaattaacg
    19321 tcaccatccc ggtatcatgt ggacgagggg agacctcact gaaaatcacc tcatcaccac
    19381 agacaaacag ctcgacacca aacagaccat aaccgcccag tgccagcacc actttacggg
    19441 caatctcctg cgcacgttca agggcaagcg ggctcatttg ctgtggttgc caggattcac
    19501 ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca gaaatggacg ccatccaccg
    19561 cgctgacggt tagcagggta atttcgaagt caaacttaac gacgccttca acaattacgc
    19621 ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca tgcctgagca agttgctctg
    19681 cagagcgaat aaacgtctgc cccttgccgg aagagctcat caccggtttt acaatgcagg
    19741 gatagccaat ggcggcaacc gcctcgcgga aaaggctttc gctatcggca aaacgataag
    19801 tggaagtggg cagctgcagc tcttctgccg ccaggcgacg gataccctcg cgattcatcg
    19861 ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc ttcctcttca agttggatca
    19921 gcatatcggt ggcaatagct tcgatctccg gcacgatata atgtggtttt tccagttcaa
    19981 ccacacggcg taatgcatca ccatcaagca tattaatgac atgggagcga tgcgcgacat
    20041 gcatggctgg tgcgtcggca tagcgatcga cggcaatcac ctctacgccg agacgctgac
    20101 actcgattgc cacttcttta cccagttcac cggagcctaa taacatcacg cgagttgctg
    20161 ccggacgcag cgcagtgcct aataacgtca taacgaatat cccctgatta attccgcgcg
    20221 cagtataaac gaaaacgttt gcgtgtgtct ttattgctga tgctgatttc tcaaccgaaa
    20281 agaaatatac tgatataaaa tcacagttat tatgagaggc tgcaaatggc ggttgaagtt
    20341 aaatacgtag tcattcgtga gggcgaagag aaaatgtcgt ttaccagcaa aaaggaagcc
    20401 gatgcttatg acaaaatgct tgatacggcg gatctgcttg acacctggct gacaaattct
    20461 ccagtgcaaa tggaagacga gcaacgtgaa gccctttcgc tatggctggc agaacaaaaa
    20521 gatgtgctga gcaccattct gaaaaccggc aaattaccgt ccccacaggt cgtcggtgct
    20581 gaatccgaag aggaagatgc ctcacacgcg gcctgaagca acttgcgctc ctgacaattt
    20641 gtaccatgct ttctcctgaa tcattgtgct atggagaaaa acatgaaaaa agaggggcgt
    20701 ttttagggcg gttgttggtt tctgcctgcg catcagtttt cgctgccaat aatgaaacca
    20761 gcaagtcggt cactttccca aagtgtgaag gtctggatgc tgccggaatt gccgcgagcg
    20821 taaaacgtga ttatcaacaa aatcgcgtgg cgcgctgggc agatgatcaa aaaattgtcg
    20881 gtcaggccga tcccgtggcc tgggtcagtt tgcaggacat tcagggtaaa gatgataaat
    20941 ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat tcattaccag gtcagcgtgg
    21001 actgcaaagc gggaatggcg gaatatcagc ggcgttaatg gcttctgata attcgattga
    21061 ccgttggcgg atgcggcgcg agtgccttgt ccgccccaga acgcaccaca atcgtaggcc
    21121 tgagaagacg caacagcgtc gcatcgggca atcagcatca aacaatgcca ctcaaaacga
    21181 caaactactt tcacctaaaa tacccttatc acctgatata cagtaaatac cttacctggc
    21241 gcgaatttct ttgaataacg gcctaccgcc ccgcttattt gcgcatcttt tgtctcgata
    21301 tacattttct gacatcctca tccgctatgc tacaggcact aaaaatggtt attgaggagc
    21361 aaatatggct aactggttaa atcaactgca atccctgctt gggcaaagca gttcttctac
    21421 ctcctcgtct gcggatcagg gattgggcaa actgttagtg ccaggcgcat taggcggact
    21481 ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt cttacgaaat atggcaccaa
    21541 cgcgttactg gttggcggcg gagcggtagc gggtacggtg ctgtggaata aatacaaaga
    21601 taaaattcgc gcggcgcatc aggacgaacc gcagtttggc gcgcaaagta cgccgctgga
    21661 tgagcgtacg gaacgtttga tccttgcgct ggtctttgcc gctaaaagtg atggtcatat
    21721 tgatgccaat gaacgtgcgg caatcgacca gcaattgcgt gaagccggcg tggaagagca
    21781 ggggcgtgta ctcattgagc aggtaatcga acaaccgctg gatccacaac gcctggctac
    21841 cggggtccgc aatgaagaag aggcgctgga aatctatttc ctgagctgcg cggctattga
    21901 tattgaccat tttatggaac gcagttacct gaatgcactg ggtgacgcgc tgaaaatccc
    21961 ccaggatgtt cgtgaaggca ttgaacgcga tctcgaacag caaaaacgca ctttggcgga
    22021 ataattccgc tctctggata gcctgaagtt gactatttcg ggctatccag aaccgcacag
    22081 ccatttgaac aggctgattt tctctgtctg cataacattc gatatccgcg ccattacctg
    22141 tcatcatcta agcaatgacg accctgtttc gcttgcatcc ccggtgagtt ttgccaccct
    22201 tataagatgt ttcaaccaga aagaacaata acatgctacc aaaagccgcc cgcattcccc
    22261 acgccatgac gcttcatggc gatacgcgca tcgataatta ctactggctg cgggacgata
    22321 cgcgttctca gccagaagtc ctggactacc tgcaacaaga aaatagttac ggtcatcggg
    22381 tgatggcctc acaacaagcc ttgcaggatc gcatcttaaa ggaaatcatc gaccgcattc
    22441 cgcaaagaga agtttctgcg ccctacataa aaaatggcta ccgctatcgg catatttatg
    22501 aaccaggctg tgaatatgct atctaccagc gtcaatcggc attcagcgaa gagtgggatg
    22561 agtgggaaac attgctcgat gccaacaagc gcgcagctca tagtgagttt tattcgatgg
    22621 gcggaatggc gattacgccc gataacacca ttatggcgct ggcagaagat tttctttccc
    22681 gacgccagta cggcattcgt tttcgtaatc tggaaacagg taactggtac ccggaactgc
    22741 tggataacgt tgaacccagc tttgtctggg caaatgactc ctggactttc tactatgttc
    22801 gcaagcatcc ggtgacgctg ctgccttatc aggtctggcg tcacgccatc ggtacgccag
    22861 catcgcaaga tacactgatc tacgaagaaa aagacgatac ctattatgtc agcctgcata
    22921 aaacgacgtc gaagcactat gtcgtcattc atctggccag cgccaccacc agtgaagttc
    22981 gcctgctgga cgcggaaatg gccgatgccg agccgtttgt ttttctgccg cgccgcaaag
    23041 atcacgaata cagccttgat cactaccagc atcgttttta tctgcgttcc aatcgccacg
    23101 gcaaaaactt tggcttatac cgtacccgta tgcgtgatga gcaacagtgg gaagagttaa
    23161 ttccgccacg cgaaaacatc atgctggaag ggtttacgct gtttaccgac tggctggtgg
    23221 ttgaagagcg tcagcgcggg ttaaccagtt tgcgccaaat taaccgcaag acccgggaag
    23281 tcattggcat tgcctttgat gatccggcct atgtgacctg gattgcctac aatccagaac
    23341 ctgaaaccgc gcgattgcgt tatggttatt cttccatgac cacaccagac actttgtttg
    23401 aactggatat ggataccggt gagcgtcgtg tattaaaaca aacagaagtg cccggttttg
    23461 atgcggcgaa ttaccgcagt gaacacctgt ggatagtcgc ccgtgatggc gtcgaagttc
    23521 cggtttcgct ggtctatcat cgcaaacatt ttcgcaaagg acacaacccg ttgctggtgt
    23581 atggctatgg ttcttacggc gcaagtattg atgccgattt cagttttagc cgcttgagtt
    23641 tgttagatcg tggctttgtc tacgccattg tccatgttcg cggcggtggt gagctggggc
    23701 aacaatggta cgaagacggt aaatttctga agaagaaaaa tacgtttaat gattatcttg
    23761 atgcctgcga tgcattgtta aaactgggct atggctctcc ttcgctctgt tatgcgatgg
    23821 gcggaagtgc ggggggcatg ttgatgggcg ttgcaattaa ttaacgcccg gaattattcc
    23881 acggcgttat cgcccaggta ccgtttgttg atgttgtaac aacgatgctt gatgaatcaa
    23941 ttcctcttac cactggtgag tttgaagagt gggggaatcc gcagggtccg caatattacg
    24001 agtacatgaa aagctacagc ccgtatgaca acgtcaccgc acaggcttat ccgcatttac
    24061 tggtaacgac cggtttacac gattctcagg tgcaatattg ggaaccggca aaatgggtcg
    24121 ctaaattgcg cgagctgaaa accgatgacc atcttttatt gctctgtacc gacatggact
    24181 caggccatgg cggtaaatct ggccgcttta aatcgtacga aggcgtagcg atggaatatg
    24241 cttttctggt cgcgctggcg cagggaacat tacccgctac gcctgcggat taagtatttt
    24301 ccagataatg tttcagtgtt aaacgcagct ccgggctcat gctgtccagg ttattaaata
    24361 accagcgcag atagcccgga tcgcgttcgg caacgtcgga aaccgctttg ccacggtatt
    24421 tgccaaaggt gaaggtcgtc atcaacgacg gacgtccggt gatatcggcc atctgttctg
    24481 ccgtccagcc ggaggtgttc ataatatcga taagcaacgc ggcggtgata taacagtcat
    24541 acaacgcgcg gtgatgatgc aggcccggcg gtgtctgtac attgagcttg cgtgttttat
    24601 ataacgccat attgctgtac ttgagccctg gccacaaacg acgggccagt ttcatagtgc
    24661 aaatccactc accgggcatc tcaggcagta cgcggcggtc aaagctggcg ttgtgcgcga
    24721 cataccattc actaccgtaa tagtgtggga tcacatcttc aatccacggt ttatcggcga
    24781 ccatggcttc ggtgatgcga tgaatcgcca tcgcttgtgg actaatagga cgatcggggc
    24841 gcaccaggag gctcatgggg ttgacgattt ttccgtcaat gacatcaaca gaggcaatct
    24901 caacgatccc tccctgcaaa ccgcaggttt ctgtatcgat aatgcgcaac atggtatgct
    24961 ccaggccgaa aaagcctagc gtaatggaat gatatcgcct tgccaaccct gtgatgaacg
    25021 ctgaccgact aacaataatg aaccacgatc ggcgcgaacg atgagacgac catattcatc
    25081 ccatagtgca ctgttaccac gggcatttgc catcagcacg gcaatcgagt actgatgtga
    25141 aaaaaattgt aagcgacggg ccgacgccag caggtcgggt tctcccagac attgccctgt
    25201 cgtaaacagt gaacaggtcg ggtccatatc cataccttgc ggttgttcat cgaccaccgt
    25261 gatggttctg gaacgacggc ccagacatgc accatgactc tgatggtaga tcccaggcgt
    25321 ttttcgccac ggtgcgaaca ccgcaattcc aggaataaag cgatcgttat attcaacggg
    25381 aaggccggca atgatggtca ttcgccaggt cgttgcggca taacaaagcg ggtctaacag
    25441 tgaaagatcg ggtggggcag gaagggcacg tcgcgaataa tcacacccca gtaaagaaag
    25501 cgatggaaaa actaacagct gacattgttg cctggcggcg gccctcacaa actccagatg
    25561 atgagctacc tgttcggtga gtgaggtttt acgaggctca tattgcgctg ctgcaacttt
    25621 ccagaacgac ataatgactt ccttttcaaa atgactgcaa attatggggg cggtttagaa
    25681 aatactcata atttagcagg cgttatgtaa gaaagtgtaa ctctgtataa gttttattta
    25741 agtttgggct cgtaaggtaa acgtgacaga ttgaccgaag ccaaacggtg ggcaataagc
    25801 cgctcgcgaa accagctgcg caaatgttct ggctgttcac gttcaaccgc ttcagcgatc
    25861 accggcatat tgtagcgttc tttaaaagcc accccggccg ccgccaaatc gacattcact
    25921 ttatccattt ctgtttgatc cagtttagcc agattcttca gcattcttaa tctcctcatc
    25981 attcgcgggc aaaagttact aagagtgagc ctggatggca agccggaatt gatgatcctc
    26041 gactcagaca ttagacaagg ttatgctctg taaaaaagtt attacaaaaa ggaatagata
    26101 atatggcttc aactgcacgc tcccttcgct acgcgctggc aatcctgacc acttcgttgg
    26161 tgacaccttc cgtctgggca catgcgcatt taacgcatca gtatcctgcg gcaaacgcgc
    26221 aagtgacagc tgccccgcag gcaatcactt taaacttctc ggaaggcgtt gaaacaggat
    26281 tcagtggtgc aaaaatcacg gggccaaaaa acgaaaatat taaaacatta ccagcgaagc
    26341 ggaatgagca ggaccaaaag caactgattg ttccactggc cgattcgctg aaacccggaa
    26401 cctacaccgt tgactggcat gttgtttcgg tggacgggca taaaacgaaa ggacactaca
    26461 cctttagcgt gaaataaatc atgctggcgt ttacctggat cgcgctgcga tttatccatt
    26521 tcacctcgtt gatgctggtc tttggctttg caatgtatgg tgcctggctg gcccccttaa
    26581 tgattcgtcg cttgctgacg aagcgttctc tgcgtttaca acagcacgct gccgtctgga
    26641 gtttgatcag tgccactgca atgctcgccg ttcagggcgg gttgatggga acgggatgga
    26701 cagatgtatt ttcgccaaac atttggcagg ctgttttgca aacgcagttt ggtggcgtct
    26761 ggttatggca aattgttctc gccctcgtaa cgttgatcgt cgcccttatg caaccgcgga
    26821 atatgccacg tttgctgttt atgctcacta ccgcgcaatt tattctgctg gcgggagtgg
    26881 ggcatgcgac gctgaatgaa ggggtaacag cgaaaatcca tcagactaat cacgcgattc
    26941 acctgatttg tgcggcggcg tggtttgggg gattactgcc ggtgctctgg tgtatgcaac
    27001 tcatcaaagg ccgttggcga caccaggcta ttcaggcgct gatgcgtttt tcctggtgcg
    27061 ggcattttgc ggtgatcggc gtactggcaa gcggcgtgct taatgcattg ttgattaccg
    27121 gatttccccc cacgcttacg acttactggg gccaactctt attgctcaaa gccatcttgg
    27181 tgatgattat ggtggtcatt gcgctggcta atcgttatgt tcttgtaccg cgcatgaggc
    27241 aggatgaaga tcgtgcggca ccctggtttg tgtggatgac taaactcgaa tgggcgatag
    27301 gtgcggtagt gctggtgatc atcagcctgc ttgcgactct cgaacctttt tgatggatta
    27361 cgaaaatgat gaaaaaaagt attctggcgt ttctgttact caccagttct gcagcggcgc
    27421 tggcggctcc acaggtgata accgtgagtc gttttgaagt gggtaaagac aaatgggcgt
    27481 ttaatcgcga agaggtaatg ctgacttgcc gaccgggtaa tgctttgtat gtcatcaacc
    27541 caagtaccct cgtgcagtat cctttaaacg atatcgcaca aaaggaagtt gccagtggga
    27601 agaccaatgc ccaacccatt tcggtgattc agattgatga tcctaacaat cccggcgaaa
    27661 aaatgagtct ggcaccgttt atagaacgag ctgaaaaact ctgttaatta cctaaaatag
    27721 ccttttgatt tccaataaaa aaaccgcctc agttctttca ccagaacggg cggtttttaa
    27781 catttaagct gatgaccacc gcgcttttta ttgaccattt tgcacgcaaa ctggaaaacc
    27841 tggcgtcgtc atctattctt aaagggcaag gcaactaagc ctgcattaat gccaactttt
    27901 agcgcacggc tctctcccaa gagccatttc cctggaccga atacaggaat cgtattcggt
    27961 ctttttttaa ttgcatttga aatcaattag ttgcaaatgt ctcccacgaa attccccgaa
    28021 atttactcga atttctgtat tccggtcttt ttggttatat cacaaccaaa atacatttaa
    28081 caatccattt acgttaaaat cagagcagta agtacgtttt ttctctctca tcaagataca
    28141 tttttgttgt cttctccgat gtgtggccaa gtagacgctg agcaaattct tctccacatg
    28201 tttctttgta caatcgtcca gccaggcttc tgatctcgtg aaaagttggt gggttttcac
    28261 tgaactggat acctgtgaat tttctggctg cgacaaattt ttttgtcagg ccatccgggt
    28321 gaatgctgcc gtcagggctg ttttttctaa tcccggcact gattagataa tctccccggc
    28381 ttaccaggcg gcatcgttca actactgtac caagccgtag accagcgaca ggaaggctga
    28441 gtgacagggg gatagcaatc a
//
LOCUS       DADLSB010000035        27696 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.035, whole genome shotgun sequence.
ACCESSION   DADLSB010000035 DADLSB010000000
VERSION     DADLSB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.035"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..613
                     /locus_tag="JN331_001539"
     CDS             <1..613
                     /locus_tag="JN331_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7259716.1"
                     /translation="MDIHSYHCEGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQ
                     ELTEVAELITSPEAIKNNLAVAFLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHAS
                     LQQRWSVADMAATIPCSEAWLRRLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGE
                     VADTLNFFDSFHFSKAFKHKFGYAPSAVLKNTDQHPTDASPHN"
     gene            complement(610..1424)
                     /locus_tag="JN331_001540"
                     /pseudo
     CDS             complement(610..1424)
                     /locus_tag="JN331_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(1424..3040)
                     /locus_tag="JN331_001541"
     CDS             complement(1424..3040)
                     /locus_tag="JN331_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY7259717.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3335..4009
                     /locus_tag="JN331_001542"
     CDS             3335..4009
                     /locus_tag="JN331_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7259718.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(4048..5709)
                     /locus_tag="JN331_001543"
     CDS             complement(4048..5709)
                     /locus_tag="JN331_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY7259719.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQQVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(5905..6333)
                     /gene="ibpB"
                     /locus_tag="JN331_001544"
     CDS             complement(5905..6333)
                     /gene="ibpB"
                     /locus_tag="JN331_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY7259720.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6445..6858)
                     /gene="ibpA"
                     /locus_tag="JN331_001545"
     CDS             complement(6445..6858)
                     /gene="ibpA"
                     /locus_tag="JN331_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY7259721.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            7163..7495
                     /locus_tag="JN331_001546"
     CDS             7163..7495
                     /locus_tag="JN331_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY7259722.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7497..8747)
                     /locus_tag="JN331_001547"
     CDS             complement(7497..8747)
                     /locus_tag="JN331_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY7259723.1"
                     /translation="MMAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWH
                     YDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVM
                     HQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            8812..9912
                     /gene="cbrA"
                     /locus_tag="JN331_001548"
     CDS             8812..9912
                     /gene="cbrA"
                     /locus_tag="JN331_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAY7259724.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSDAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(9873..11210)
                     /locus_tag="JN331_001549"
     CDS             complement(9873..11210)
                     /locus_tag="JN331_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7259725.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11285..12433)
                     /gene="dgoD"
                     /locus_tag="JN331_001550"
     CDS             complement(11285..12433)
                     /gene="dgoD"
                     /locus_tag="JN331_001550"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAY7259726.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPFGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(12430..13047)
                     /locus_tag="JN331_001551"
     CDS             complement(12430..13047)
                     /locus_tag="JN331_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY7259727.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13031..13909)
                     /locus_tag="JN331_001552"
     CDS             complement(13031..13909)
                     /locus_tag="JN331_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY7259728.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(13906..14595)
                     /gene="dgoR"
                     /locus_tag="JN331_001553"
     CDS             complement(13906..14595)
                     /gene="dgoR"
                     /locus_tag="JN331_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY7259729.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14873..15529
                     /locus_tag="JN331_001554"
     CDS             14873..15529
                     /locus_tag="JN331_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259730.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15575..16388)
                     /gene="yidA"
                     /locus_tag="JN331_001555"
                     /pseudo
     CDS             complement(15575..16388)
                     /gene="yidA"
                     /locus_tag="JN331_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
     gene            complement(16502..16909)
                     /locus_tag="JN331_001556"
     CDS             complement(16502..16909)
                     /locus_tag="JN331_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY7259731.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(17130..19544)
                     /gene="gyrB"
                     /locus_tag="JN331_001557"
     CDS             complement(17130..19544)
                     /gene="gyrB"
                     /locus_tag="JN331_001557"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY7259732.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19573..20646)
                     /gene="recF"
                     /locus_tag="JN331_001558"
     CDS             complement(19573..20646)
                     /gene="recF"
                     /locus_tag="JN331_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY7259733.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPEFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20646..21746)
                     /gene="dnaN"
                     /locus_tag="JN331_001559"
     CDS             complement(20646..21746)
                     /gene="dnaN"
                     /locus_tag="JN331_001559"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY7259734.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21751..23154)
                     /gene="dnaA"
                     /locus_tag="JN331_001560"
     CDS             complement(21751..23154)
                     /gene="dnaA"
                     /locus_tag="JN331_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY7259735.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23451..23531
                     /locus_tag="JN331_001561"
                     /pseudo
     CDS             23451..23531
                     /locus_tag="JN331_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23761..23901
                     /gene="rpmH"
                     /locus_tag="JN331_001562"
     CDS             23761..23901
                     /gene="rpmH"
                     /locus_tag="JN331_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY7259736.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23918..24277
                     /gene="rnpA"
                     /locus_tag="JN331_001563"
     CDS             23918..24277
                     /gene="rnpA"
                     /locus_tag="JN331_001563"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY7259737.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24241..24498
                     /gene="yidD"
                     /locus_tag="JN331_001564"
     CDS             24241..24498
                     /gene="yidD"
                     /locus_tag="JN331_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY7259738.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24501..26147
                     /gene="yidC"
                     /locus_tag="JN331_001565"
     CDS             24501..26147
                     /gene="yidC"
                     /locus_tag="JN331_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY7259739.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26253..27617
                     /gene="mnmE"
                     /locus_tag="JN331_001566"
     CDS             26253..27617
                     /gene="mnmE"
                     /locus_tag="JN331_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY7259740.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEACQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6699 a         7465 c         7050 g         6482 t
ORIGIN      
        1 aatggatatt cactcttatc actgtgaagg tttagtctgg gaacagtact ggatggaatt
       61 tacccccacc agtatgatgg atattcccgt tggtcagcaa agcgttattt ataatggcga
      121 aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag aagcaataaa
      181 aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgta
      241 cgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca
      301 tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga
      361 agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct
      421 cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact cggttggcga
      481 agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaaacataa
      541 atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa cggatgccag
      601 tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca actcatccag
      661 aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg cctgccacag
      721 gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg ccacttgctg
      781 actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt caggccgtag
      841 tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat ccggctttca
      901 ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag acgacgcgag
      961 gcttctgcga caattgctgc cgggttggag tagttgagca tccaggcatt tggtgaatat
     1021 ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt
     1081 ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat tttttcatcc
     1141 agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca tgacaaaatc aacgtcgctg
     1201 aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt ttctttaagg
     1261 atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc ataaaatttc
     1321 agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat gcctggcgta
     1381 aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact gcctccgtaa
     1441 tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt tcacgcaatt
     1501 gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag acgccgtgcg
     1561 ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc agtgcaatac
     1621 gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc agggcttgca
     1681 ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt tggcctcgcg
     1741 aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt cccggcgtgc
     1801 acatgttgaa ctgcagaatc agggtgcgga aaaccacgaa gtacagcagg gtaaagcaca
     1861 acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc gggatccagt
     1921 tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca ccaaagagat
     1981 acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc aacggtgaaa
     2041 tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg gttaaggttg
     2101 cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg aagtacatcg
     2161 ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca aaaccgcctt
     2221 ccgggaacaa cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc
     2281 agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt ccgtagatga
     2341 agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa
     2401 gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt acttttggcc
     2461 agccgagcaa tgtcagccag gcacagggga tcatcaccag gaaggcgata atcaccacat
     2521 aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac agacggttat
     2581 gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta tcgagggttt
     2641 taatcccggc catcattgtc agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc
     2701 cgaagtagct tccccaggtc attcccatcg cattgatgaa atagttccag gtcaggaaac
     2761 tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca atgggtaaac
     2821 cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct tcaataatgt
     2881 gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca aacatcgggt
     2941 tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc agcactggcg
     3001 tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc tcttttctca
     3061 attctgtaag taggaagcct gattggttat gcggcaggcc tggagcgagg ataggaagat
     3121 gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc ttttcgttgg
     3181 gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg tacacagcat
     3241 cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt gtgggcgcat
     3301 tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca ttgcggagcg
     3361 gttaagaatt cgacttaatt ccgcggattt cacgctaaac agccttcttc ccggtgaaaa
     3421 aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca ttgacctgct
     3481 ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga
     3541 tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac ggcagggaaa
     3601 aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg ccattgccag
     3661 ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga
     3721 agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc gtaacctttc
     3781 gctgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat
     3841 tggcggtaat tatgaaagcc tgacgccggt gctcgccgat agactgctgg cgcggcaaat
     3901 gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg
     3961 ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg tggagtacca
     4021 tttacggcga ctccacccgg aaaagagtta accgatactc cagaagagca ccgccagtaa
     4081 ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt aagagagcgc
     4141 cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat
     4201 ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag
     4261 aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag
     4321 cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac caacgaccga
     4381 gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg gcataaacca
     4441 gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc
     4501 cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa tagaacctaa
     4561 caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg
     4621 ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccaggtt
     4681 gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg
     4741 gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt
     4801 tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt
     4861 agacagcgag gtatcgacct cctgaccaat cacctgttgc gcattatgta aatccgctgg
     4921 ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg aaggtacttt
     4981 tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac
     5041 atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat
     5101 cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct cgacattgac
     5161 gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc cgaatggata
     5221 cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac ccaggtcgcg
     5281 caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg agaaaatccc
     5341 cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc
     5401 gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc
     5461 aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca ggccaaattc
     5521 ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc
     5581 gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac
     5641 gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg ttaatgctat
     5701 atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt atggctgaat
     5761 cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc
     5821 ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc cccaccagaa
     5881 tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat
     5941 acgctgcgct gcgatgggtt caggctcatt acgaattaaa tcaatatgca gtaaaccgtt
     6001 tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca ggctaaatgg
     6061 ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct ccggcgtgcc
     6121 ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac ggaaacctgc
     6181 cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa
     6241 gctctgactt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg
     6301 acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt
     6361 aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag
     6421 gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt ttttcgcttc
     6481 cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag ccccacgaac
     6541 atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga tgccctggta
     6601 cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc
     6661 ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta
     6721 atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg
     6781 gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga
     6841 taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta agaccttcca
     6901 caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga ctacgatgta
     6961 aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt gcagtttatg
     7021 gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg
     7081 cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag gccaacaccc
     7141 acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca tgatatgcag
     7201 cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg ggaaagaagg
     7261 aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa ccaactgggg
     7321 cactaagtcg ctggcgattc tcgatatgcc gtttaccgct gtgatggaca cgcttttact
     7381 gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa aaagcgaagc
     7441 caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata actgatttag
     7501 cgatcagttt cggtgatcca cagctggcca ccttccatcc acgccagcca ttgaccatcc
     7561 ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt taaatactca
     7621 acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa gcccaaccat
     7681 tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg ggttaacttg
     7741 cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt atcatcacgc
     7801 attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg cgggacgttg
     7861 actaaccccg gataagcccg atgatgggta aaggttaaac gtcgctgcac gacgccacgc
     7921 ggtggcgcgg gtagggttgt ttccgttcca cttaacggcg catcacctgc cgatttccag
     7981 ctagcttcat cttgcggtaa ctcaacgata aatagctccg gcactttctc gccctttggc
     8041 gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta cccatccttc ttcataagca
     8101 cgattgattt catcactgcc aggctgcggc gtgggcgtgg ttttactcac cagcacacac
     8161 cagtggctac cgctgtattc acgcggatgt tgcttttgta cgttgaccgg gccaaacggt
     8221 gcagcaaccc cgacgtttcg caaatccagc gccggatcga gttgatgcat tacatggtcg
     8281 ttataggtaa agctccccct ttcaccgttc gggctaaaga catgcacatg gctgccaccg
     8341 cgcagcgctc ctggcgtata cggcgcggta atatccattg catcgagatt gctcaccttg
     8401 ccgccttcag caatgactcc gcgtcgatga tggaagtcgt aatgccatgt ttcatcagga
     8461 ttttcagggc cgtgaataaa gacatatttc tctgacttcg gatgaacggt caccacgccg
     8521 acgtgtgcgc cctgtgacgc gcgatagata acctcgacct cgcctgtatg gatattcaca
     8581 cgctcaatgg tctcgccggt aaacgacgcc ccagaaggac gcacgtcaaa taccagccac
     8641 tggctgtcgg gcgtccaggt attggtattg gtgagcaggt gattacgggg agcaaaggtt
     8701 atctgtttca tggcgcggtc ctgatacaaa acggttcccg ccatcatact tcacaaggac
     8761 ttcactttca gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat
     8821 ttcgacgtgg cgattattgg cctcggcccg gcagggtcgg cgttggcacg aaagttagcc
     8881 ggcaaaatgc aggtgatcgc gctggataaa aagcaccagt gcggtactga aggtttcagc
     8941 aaaccttgcg gcggtctgct ggcaccggac gcgcagcgat cttttattcg cgatggactg
     9001 acgcttcctg tcgatgtgat cgccaatcca cagattttca gcgtcaaaac tgtcgacgtc
     9061 gccgcatcgc tcacgcgtaa ctaccagcga agctatatca atattaatcg ccatgctttc
     9121 gacttgtgga tgaaatcgct gatccccgcc agcgttgagg tttatcacga tagcctgtgc
     9181 cgaaaaatct ggcgtgagga tgataaatgg catgtcattt ttcgtgcaga cggctgggag
     9241 caacatatta ccgcccgcta tctggtcggt gccgatggcg caaactcgat ggtgcggcga
     9301 catctctacc cggatcatca aatccgtaaa tatgtcgcta tccagcagtg gttcgcagag
     9361 aaacatccgg tgccgttcta ctcctgcatc tttgataatg cgataactga ctgttactca
     9421 tggagtatca gcaaagatgg ttattttatc tttggcggtg cctatccaat gaaagacggt
     9481 cagacgcgtt tcacgacgct gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa
     9541 gcggtgaaaa gtgagaaatg cacggtgttg tttccctcac gctggcagga ttttgtctgt
     9601 ggtaaggaca acgcctttct gattggtgaa gcggcgggat ttatcagcgc cagctcgctg
     9661 gaggggatta gctatgcgct ggatagcgca gagattctgc gtgcggtgtt cctgaaacag
     9721 ccggagaaga gcgacgccgc ttactggcgc gccacacgca aactgcgttt aaaactcttc
     9781 ggcaagatag taaaaagccg atgcctgacc gcaccggctt taagaaagtg gattatgcgc
     9841 agtggtgtgg cgcatattcc acagttgaaa gattatccaa cgcgcttcac atcacccacc
     9901 agcaggatgt aagagagcgc gccaatcaac gcgacggcgg agatataaac cagtgcaggc
     9961 gcgaaaccgt aaccctgcgc caggtacccc accaccagcg gaacggtgat gccgcccaga
    10021 ccaccggcga agttaaacac gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa
    10081 gagaccagcg accaggtaat cgaagcaaaa ccgttgccga agaatgccag cgccatcagg
    10141 cacataatca tcatcggatc gttggtgtag ttagcgccca taatgcaggt ggagatcagc
    10201 aagccgcaga taatcggcgt tttacgcgca aagcccagtg aaaagccctt acgtaccagc
    10261 agatccgcga cccagccaga gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg
    10321 gtcataaagc ctgctttcag cgccgtgatt cctttttcct gggttaaata gttcgggaac
    10381 caggttaaga aaaaccacag tgcagaagcc accgcaaatt gcccaagata aacgccgatc
    10441 agtttacgat ggaacaccag tttccagtct ttggctgtta acggctgacg cgcctctttc
    10501 ttcaccggcg catcaccatc caccagaccg ccgccatcac gaatgtaatc cagttcagct
    10561 ttgctgatac ctttggtcag gcgcggcggc tgataaacct taaaccaaat cagcgaccag
    10621 ataatgccga taccaccagt gacaatgaac acccagtgcc agctcaacat ctcctgaatc
    10681 cagatcagca gcggagtcaa aaacgccaga ccgacaaact gaccagacgt ataaaaacca
    10741 acggcggaag cgcgttcatg ttccgggaac cagctggtca ccatccggtt attggtcggg
    10801 aaagcgggcg cttcgaaaat accggttatc gcacgcagac caattaatga cattaatccc
    10861 gtggcaaagc cctggaataa agtcgccact gaccaaccaa atatcgcaat aaaataagtc
    10921 acgcgagaac ctacgcgatc taaaaaccaa ccgccgggga tctggcatag cgtataaagc
    10981 caagcgaagg ccgaaaatac atagcccatt tccgctttcg taatgccgaa ctcttcctga
    11041 atatgggcgg aagccacggc caggttagcg cggtcaacat aacaaatgac caccgtaata
    11101 aagatcatca ccagcgtcag ataacgccga cgccccggct ttgctgcatt aacgggaata
    11161 tccatagcga gctttctcca gattttgggc atagcgaagc cgctcaccat gccctgtaat
    11221 ttacagaggg ttatattttt gtattgctgt tttagtgccc gatgaggggc ttacgtggca
    11281 ggaattacca ctctgctacg ctgttatctt catgacgcca gagcggatta cgccagtccg
    11341 gggcattttt actgaactca ataactttag cttcgtcgat ttccacacct aagcccggtt
    11401 tcgttaacgg tttaaagaag ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt
    11461 cgagtaactc cgcgcctttg ttgtaatgaa tgcccatact ttgttcctga agtaccgcgt
    11521 tataggaaac aaagtcgata tgcaggcaag ccgccagtgc aatcggtccg aacggacagt
    11581 gcggcgcaag ggtcacatca taggcttctg ccattccagc aattttgtag cattcggtaa
    11641 taccgcctgc atgggagaga tccggttgca gaatcgaaat accgcctgcc tccagcacgc
    11701 gtttaaaatc gaaacgtgag aacatgcgct cacccgccgc cagtggaata tgcgtttgtg
    11761 ccgccaattt cgggtagtat tcggcttgtt ccgccagcac cggctcttca ataaacagcg
    11821 ggcgatacgg ctccagttct ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt
    11881 ggaaatcaag accaaactca atctgattgc caaaagcttc acgaatttgt gccacggtgt
    11941 tgactgccgc atctaccgcg cgggagttat caattagccc cagttcttca caaccgttca
    12001 gtttgaaggt atcgaagccg atttcgcgca gggttttaat gccgtcgata acatccgccg
    12061 gacgatcgcc gccgacccaa ctgtaggctt taattttgtc gcgaaccagg ccgcccatca
    12121 gttgccagac tggcgcattc agcactttgc ctttgatatc ccataacgcc tggtcgatcc
    12181 cggcgatggc gctcatcagg attggaccgc cacgataaaa tccggcgcga tacatcactt
    12241 gccataagtc gttgatgcgc gaaggatcct gaccaatcaa atagtcaccc agctcgtgaa
    12301 ctgccgcttc caccgtacgg gcgcggcctt caatcacggg ctcgccccaa ccgaccacgc
    12361 cttcatcggt ttcaattttc aggaacatcc agcggggagg taaacgatac gtggtaattt
    12421 tggtgatttt cattgcactg cctctcgata cgccttaaca aatgctgctg cctgctgcgc
    12481 ggtgcgttct acggactgcc cggcgcgata gagatcgctg cctaagcccg ccccaacaca
    12541 acctgcgtct atccactgcg ccaggttttc tggcgtcacg ccgccaacgg caaagactgc
    12601 gatgtccggt ggtaataccg cttttaacgc tttgatgtat tgcggaccaa aagccgatga
    12661 cggaaatatt ttcagcgcct gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt
    12721 cgcgcagccg gggcaaacgg tcatgccgta gcccaccgcg cggcggatca cttcactatg
    12781 gatattgggc gtaacgatga gctgacagcc catcctggcg agcgcatcga cctgttcagg
    12841 tttcagtacc gtacctgcgc caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg
    12901 aatgctctgc tcccattgtg gggaattcag cgggatttca accgcgtcga acccggcgtc
    12961 aatcaccgcg ccaacatgcg ccagcgcctc gtcgggcgta atgccgcgca aaatggcgat
    13021 cagcgggagt ttagtttgcc actgcatgag cgatgctcct tataccagcc tgaaatgccg
    13081 tgtcgcccgc caccgccgcc acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg
    13141 cggtcagcga tgttccggcg acaagggtga tggcgtgttg atgggtcaca tagtcgcgca
    13201 tactggcaac ctctgcgcca atcaacaaac cagagagaaa ttcgctgacc tgttcgcggg
    13261 gaagtgttcc cagcacatgc gaggcgcgaa cttcaaaaag ctgcggcaat atggcgggcg
    13321 cattaaggcc gcgctcaagg ccagccgcga aggcatcggc agagttttcc tgcggcggca
    13381 aacctgcgcc aatcaatgag tgatttaaca gtaaatgatg taattcaccg gtcatcacgg
    13441 tgcgaaaatc gttgatttgc tggctatcgg cctgcaccca tttgcaatgc gtaccgggca
    13501 taacataaag agaggaagga gccagagctc gcgcgccgat caattgtgtt tcttcgccgc
    13561 gcatcacatt gtggttatcg tcatgagaga cgcataatcc gggaataatc cagatattgt
    13621 cgccaacaga cgttaattgt tcgccaatag acgaaaaacg ggcaggaaca gataaatacg
    13681 gagcaacttt ccagccgaca ttgctgccga ccattcctgc catcaccact ggcgtatttt
    13741 cttcacgcca gtcggtcgtg acttctgcta acaccgcagc cggagatttt ccgttcaggc
    13801 gcgtgacgcc tgcttctgat tgcctgctct ccaggcagtg gtcgccctga taaagccagg
    13861 cgcgcagatt ggtcgatccc cagtcaattg cgatgtagcg agctgtcatg tgatttcctt
    13921 taaccttcgt gtcgagctgg caatcatggt aagcgccgcc tgctctgccg catcgccgtc
    13981 ctgatgccgt atcgcatcga acagcgcctt atgttcctgg agcgtttgcg gcatgttggc
    14041 ctcatcgccc atccaggttc gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct
    14101 aagttgctgt aacaccgggt tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc
    14161 cgcttcgtta aacgcttcgc ggtcctgatt gttggcaatc atctcgttca gcgccgattc
    14221 aatctgcgcc agatcgctgg aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc
    14281 caccagattt cgcacttcgc tcatggcact gataagccgt gggtcgtagt cgttttccag
    14341 cacccattgc agtacgtcag tgtcgaggta attccactgg ttacgcggtg ccacaaacgc
    14401 cccgcgataa cgtttcattt caatcagccg cttcgccatc agcgaacgga acacctcacg
    14461 gatgatgttg cgcgaggttg caaactcttc acagagctcc gcctcagccg gaagtggcga
    14521 gccaggtacg tatttgccgt gaacgatctg tttacccagc gtaatgacaa tgcgatcggt
    14581 tttattgaga gtcatggaga gtccttgtgc tctgtatgtt cttctctact ttaccccgat
    14641 cgatgcataa cgcggcaact ttgtagtacc agcgtgatga cgttcgcgtt tgccgtgtgt
    14701 gtaatgtggt acaaacttat gttgttgtac tacaatttag atcaaaaaaa gaacaatgcg
    14761 taaaaaaatg acatgcgtcg ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag
    14821 ggaaagaaga gtatgctttc aacggcttag ctactcgttt aaaggattaa tcatgaagtt
    14881 gaattttaag ggatttttta aggctgccgg tttattccca ctggcgctga tgctttcagg
    14941 ctgtatctcg tatgctctgg tttcccatac cgcaaagggt agttcaggaa agtatcaatc
    15001 gcagtcagac accatcactg ggctatcgca ggcaaaagat agtaatggaa caaaaggcta
    15061 tgtttttgta ggggaatcgc tggattacct tatcactgat ggtgccgatg acatcgttaa
    15121 gatgctcaat gatccagcac ttaaccggca caatattcag gttgccgatg acgcaagatt
    15181 tgttttaaat gcggggaaaa agaaatttac cggcacaata tcgctttact actactggaa
    15241 taacgaagaa gaaaaggcac tggcaacgca ttatggtttt gcctgtggtg ttcaacactg
    15301 taccaggtca ctggaaaacc taaaaggcac aatccatgag aaaaataaaa acatggatta
    15361 ctcaaaggtg atggcgttct accatccatt taaagtgcga ttttatgaat actattcacc
    15421 cagaggcatt ccggatggtg tttccgcagc attactgcca gtgactgtta cgctggacat
    15481 cattactgca ccgctgcaat ttctggttgt atatgcagta aaccaataat cagtaagcgg
    15541 gcaaacgcgt ttatgctgtt tgcccgccca cagattaatt cagcacatac ttctcaatag
    15601 caaacgccac gccatcttca atggttggat tttgtgacaa agttcgccac ttctttcacc
    15661 gaaggaatag cgttatccat cgccacaccg acgcctgcat attcaatcat tgcgatgtcg
    15721 ttttcctgat cgccaatcgc catgatttct tccggtttaa tacctaacac gtcggccagt
    15781 gatttcacac ccgtaccttt gttaacgcgt ttatcgagga tttcgaggaa gtacggcgca
    15841 cttttcagca cggtatattt ctctttcact tcctgcggaa tacgcgcgat agcctggtcg
    15901 aggatggcgg gttcatcaat catcatcact ttcaggaact gggtattggg gtccattttc
    15961 tccgcttcgc agaacaccag cggaattgtg gcaacgaagg attcatgcac cgtgtagtag
    16021 ctgatatcgc ggttagcggt gtacagcgtg gtgcggtcca gggcgtggaa atgagaaccg
    16081 acttcgcgag agagtttttc caggaaacga tagtcgtcat agctgagagc ggtttgcgcc
    16141 acggtgctac catcagcggc cttctgtacc agcgcgccgt tataagtaat gcagtagtcg
    16201 cccggctgtt ccatatgcag ctctttcagg tagttgtgca cacctgcata cggacgaccc
    16261 gtcgttagca cgacattcac gccacgggcg cgagctgcgg caatcgcatt tttaacggcg
    16321 ggtgaaatgg tgtgatcggg cagcagaagg gtgccatcca tatcgatagc aatgagtttg
    16381 atagccatga gttcccaagg tagattagtt cctgacccat gctaacgcga ttccgctcaa
    16441 aaatcagtac aacacccgag ggaaaagggg atgcaacgcg ggttgcatgc tccccatttg
    16501 cttagcggaa gagtttccct ttcagcagtt ccatgcctgc ggaaagcaga tcgttattgg
    16561 cttgtgctga cacttcacct tgcggtgaga gcgcatcaat aatcttcggc aattgctctg
    16621 ccagtaaact ggaagccgta ctggtatcca cgccaagttt ttgcccgaga tcggacaccg
    16681 catttgtgcc gagcgccgat tccagttgct cgccgctaac tgattgattt ctctgttgat
    16741 tactcaacca ggttgagaga atggccccta agccgccact ttgcagtttt tccagcagca
    16801 cctgaatgcc accctgctcc tcaacccaac ttaaaatcgc ctgatatttc cccgcatcgc
    16861 ctttcagaaa ggcaccgaca acttcatcaa aaagccccat gataatcacc tgtaaggcgt
    16921 tacgtgttga cccaaaaagt atagatctgc gggatgataa ttgcggattg cagaaataaa
    16981 aagggcggag atgatctccg cccttttctt atagcttctt gccggatgcg gcgtgaacgc
    17041 cttatccggc ctacaaaatc aatatattgc aggagtctcg taggactaat aagcgtagcg
    17101 catcaggcac gctcgcatgg ttaacgccat taaatatcaa tattcgccgc tttcagggcg
    17161 ttctcttcaa taaacgcacg acgcggttct acggcgtcgc ccatcagcgt ggtgaacaac
    17221 tggtcagcgg caatcgcatc cttaacggta acgcgcagca tacgacggct ttccgggtcc
    17281 atggtggttt cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg
    17341 gagaggccac gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc
    17401 ggctgacgac gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc
    17461 tcacccagcg tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga
    17521 tagtcagtat ccacaccgtg ggtacgcacg cgaacaatcg gctcgaacag gttttgttcg
    17581 gcattggtgt ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg
    17641 ctgaccagcg cgttcaccca gcgggtaacg gtctgctcat cagaaaggtc agcttccgtc
    17701 aacgtcggct gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg
    17761 ttgatcattt tctgcgtcgc gttgtactca gataccagtt tctctaacgc ttcgccagcc
    17821 aatgccggtg cactggcgtt ggtgtgcagc gttgcgccgt ccagcgcgat agagatctgg
    17881 tactgatcca tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg
    17941 tacagcggcg gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga
    18001 tagaagaagg tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg
    18061 atgatgatgc tgtgataacg tagtttgtcc gggttgtact cgtcacgacc gataccacag
    18121 ccaagcgcgg tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct
    18181 ttctcgacgt taaggatttt acccttcagc ggcagaatcg cttggttctt gcggttacgc
    18241 ccctgcttcg cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc
    18301 ggatcgcgtt cctggcagtc tgccagtttg cccggcaggc cagctaagtc gagcgcacct
    18361 ttacggcggg tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata
    18421 attttgccaa ccacgatttt cgcatcagtt gggttttcca gcaggtattc cgccagcagt
    18481 tcgttcatct gctgttcaac cgccgatttc acctcagaag aaaccagttt gtctttggtc
    18541 tgggaggaga atttcgggtc cggcactttt acggaaacga ccgcaatcag gccttcacgc
    18601 gcatcgtcac cggtggcgct gactttggct tttttgctgt agccttcttt gtccatgtag
    18661 gcattcaggg tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc
    18721 tgcggaatgt tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcagc
    18781 gccacttcaa cgccaatacc gtctttttcg gtggagaagt agaagatatt tgggtggatc
    18841 ggcgttttgt tcttgttcag atattcaacg aacgctttga tgccgccttc atagtggaag
    18901 tggtcttctt tgccgtcacg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac
    18961 gacaactcac gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag
    19021 gtttcgaggc tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc
    19081 gccagcggag cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc
    19141 tgaataacca gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc
    19201 agaccgccgg acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc
    19261 atgatcactt ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc
    19321 ccgtcatcct gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag
    19381 tgacccgcga gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga
    19441 ccggtgccgt catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc
    19501 cctttcagga ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca
    19561 tttatacttg ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc
    19621 gtccgacatg tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt
    19681 cgcttttaag cggctggcaa gcaacccgcg acgctcatca tcaagctcag aggcaaaatc
    19741 atctatcagg tagagacacc gccgcccgct ttcacgagtg aggaactctc cttgcgccag
    19801 acgtaaggcg cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc
    19861 accgtcggcg cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg
    19921 gcgatcgcgt tcaaaattac gttccagcac ctcagcgtac tctgtctctt tctcccagcc
    19981 gcgctggaaa gagaaagtca gagaaaactc agggagaaat tgcttacagg tatcagccat
    20041 atcggccgcg ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg
    20101 gatcagttct ttatcccacg ggcgtagctg ttcgtaacga gtcacctggc gcagcgccgc
    20161 attgcgctgc ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatt caggttcgtt
    20221 gtgaaagcat ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt
    20281 aaacccttct ggcgttatca actgcatcgg catcaggtgc gccagttccg cgactttatg
    20341 cccgtccgta ccgtcaatac ggactttgct gtcgccctgt ttgtctttgg ttaagccaat
    20401 cgccgtctcg cgctcttcgc cctgtaatcg cccgtggaga acaaatgcct cctgctcatg
    20461 gcgaatgacg cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat
    20521 ggcttccagc acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg
    20581 agataaagcg agatccgcgg tttcaatatt gcggaaatcg cggatcaaca agcgggtgag
    20641 ggacattaca gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca
    20701 atctgcacgc tggaaaccga atcggtcagc atcatgcgga cgttttcgca tttcagcgcg
    20761 ttcagaacat ccagcacata gctgacgttg aagccgattt ccatctccgc accgctatag
    20821 gtaacgtcga ggatctcttc cgcttcttcc tgttccgggt tgttggcagt aattttcagc
    20881 tggttttcgc tgacatacag gcgcacaccg cggaatttct cgttagagag aatcgccgcg
    20941 cgagcaaacg cctgcttgag cagatcgcag cctgcttcca gatgtttgtc cgggttcttc
    21001 ggcagaacgc gacggtaatc cgggaagcga ccatccacca gtttcgaggt gaaaataaag
    21061 tcgccaacgt gggcacgaat gttattgctg ccaatctgca cacgcagcag attgtcgccg
    21121 ccgtcgagca tacgcatcag ttcaatcacg cctttacgcg gtacgatcac cgaatggctt
    21181 ggcaaagatt gaccaattgg cattgaacag accgccagac ggtggccgtc ggttgccacg
    21241 gtgcgcagtt cttcaccttc ggtttcaaac agcataccgt ttaagtaata gcgaacatcc
    21301 tgatgcgcca tcgaaaactg ggttgcttca atcagacgct tcatcgtcgc ctgcggcagg
    21361 gtaaattcga cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta
    21421 gacagcgaaa aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgcacg
    21481 gcaatttccg cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg
    21541 gtcgtcgctc ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg
    21601 agatcagtac cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga
    21661 atcggtagcg taggacgacc acctaacgga ccgctcacct gttgtagcgg ttttaataaa
    21721 tgctcacgtt ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga
    21781 aaaatcttct ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg
    21841 aagcaccgtc gtgtggtcac gcccaccaaa cgcatcgcca atctctggca gactgtggtt
    21901 agtcagctct ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg
    21961 acgcttggaa aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat
    22021 attgtcgatg gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagcg cctcacgcac
    22081 gaagtcgatg gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc
    22141 cccttccagc tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcacc
    22201 cggcaaacga atgtcatttt cgtcggcctt tttcatcagg atcgccacac gggtttccag
    22261 ctctggcggt tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc
    22321 aacgccgttg atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc
    22381 cagcagggcg ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa
    22441 ctgaatatcg tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat
    22501 cgcgttgttt tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac
    22561 cactttggca ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt
    22621 tttacccaga cccgtgccgc cataaaggaa caacgggtta taggcaccgc cagggttatc
    22681 cgccacctgg cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc
    22741 aaacgtgtgt ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt
    22801 atcccaacct gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc
    22861 aggggccgcg acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc
    22921 gacttcaaaa cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat
    22981 attattaagg tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc
    23041 cagcgtgtta tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc
    23101 tggtaactca tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga
    23161 ctccactcga acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac
    23221 gtcgaaaaga ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat
    23281 caagatcctg caaaacgctc gggaccgcgg atcatagcct aaactgcgca agagatcttc
    23341 tgtttctcac agattttctc tatttatcca caggactttc cagaactcgc taagtgtaaa
    23401 cgatcctgcc gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag
    23461 acaaaaattg gcttaattga tctaataaag atccaggacg atccttgcgc tttacccatc
    23521 agcccgtata atcctccacc cggcgcgcca tgctggtttc cactggtgtg aggtcgtaca
    23581 ttttccctgc gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccggt
    23641 gtgtacaatt attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc
    23701 atttttcggc ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc
    23761 atgaaacgca cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct
    23821 cgtatggcta ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct
    23881 cgtctgaccg tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag
    23941 ggagttacgc ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc
    24001 tggcacgccg caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg
    24061 tcttacagtc gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct
    24121 gacgcgtgaa agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt
    24181 ggcgaaaaaa ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt
    24241 atggcgccgc cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa
    24301 cgcctgatta gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac
    24361 ggaattgagg cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc
    24421 gtattaaaat gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt
    24481 gataccagag aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt
    24541 tcgtgtcttt catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc
    24601 aacagaccac gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg
    24661 ccagtggcca ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca
    24721 cccgtggtgg tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta
    24781 cccagccgtt ccagctgttg gaaacttcac cgcagtttat ttatcaggca cagagcggtc
    24841 tgaccggtcg tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg
    24901 aaaaagacgc ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata
    24961 ccgacgcggc aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg
    25021 tcaacgtcaa ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tcctcgtttg
    25081 gtcagttgaa gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg
    25141 cactgcacac cttccgtggc gcggcgtact ccacgcctga cgagaagtat gagaaataca
    25201 agttcgatac cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg
    25261 cgatgctgca acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact
    25321 tctataccgc taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac
    25381 tggttcagcc tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc
    25441 aggacaaaat ggcagctgtt gctccgcacc tagatctgac cgttgattac ggttggttgt
    25501 ggttcatctc tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact
    25561 ggggcttctc cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca
    25621 aagcgcagta cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc
    25681 gtgagcgtct gggcgatgac aaacagcgta tcagtcagga aatgatggcg ctgtacaaag
    25741 ctgagaaggt taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc
    25801 tggcgttgta ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt
    25861 ggatccacga cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa
    25921 cgatgttctt cattcagaag atgtcgccga ccactgtgac cgacccgatg cagcagaaga
    25981 tcatgacctt tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg
    26041 tactgtacta tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg
    26101 gtctggaaaa acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt
    26161 cgctaaaata agggcggtca gttgaccgcc ttttttcttt tcgtagggcg gataagcacc
    26221 gcgcatccgc cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc
    26281 aggccacgcc tccgggacgt ggcggcgttg gcatcctgcg catctccggc ttcaaagccc
    26341 gtgaagttgc cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc
    26401 cgtttaaaga cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc
    26461 cgaactcgtt caccggcgaa gatgtgctgg aactgcaagg tcatggcggt ccggtgatcc
    26521 tcgacctgct gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg
    26581 agttttccga acgcgcgttt cttaacgata aacttgactt agcccaggcc gaggcgattg
    26641 ccgatcttat cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag
    26701 gcgcattctc cgcacgggtt aatcatctgg tagaagccct cacccacttg cgcatttacg
    26761 tcgaagcggc aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg
    26821 aagcccagct taatgacgtg atggccgatc tcgatgcagt gcgtgctgaa gcatgtcagg
    26881 gtagtttgtt gcgcgaaggg atgaaagtag tgattgccgg acgtcctaat gccggtaaat
    26941 cgagcctgtt aaacgcgctg gcgggccgtg aagcggcaat cgtaaccgat atcgccggaa
    27001 ccacgcgtga cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg
    27061 acactgccgg gctacgtgag gccagtgacg aagtggaacg tattggtatc gagcgcgcgt
    27121 ggcaggaaat tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acgacagacg
    27181 ccgttgatcc ggcagagatc tggccggaat ttatcgcccg tctgcccgcg aaactgccga
    27241 tcaccgtggt acgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga
    27301 acggtcacgc gttaattcgt ctctcggcaa ggactggtga aggcgtggac gtgctgcgta
    27361 accatctcaa acagagcatg ggctttgaca ccaatatgga aggcggcttc ctggcgcgcc
    27421 gtcgccactt acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac
    27481 tgttgggagc ctgggcaggt gaactgctgg cggaagagtt gcgcctggca cagcagaact
    27541 taagcgaaat caccggggaa tttacttcag acgacctgct ggggcggatt ttctccagct
    27601 tctgtattgg taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg
    27661 cttcattgtt agcactcctg ttatgcctca accctt
//
LOCUS       DADLSB010000036        27598 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.036, whole genome shotgun sequence.
ACCESSION   DADLSB010000036 DADLSB010000000
VERSION     DADLSB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.036"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..674)
                     /locus_tag="JN331_001567"
     CDS             complement(<1..674)
                     /locus_tag="JN331_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAY7259741.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGGNNFAAQGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYR"
     gene            complement(729..1670)
                     /locus_tag="JN331_001568"
     CDS             complement(729..1670)
                     /locus_tag="JN331_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7259742.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            complement(1701..2675)
                     /locus_tag="JN331_001569"
     CDS             complement(1701..2675)
                     /locus_tag="JN331_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7259743.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            complement(2672..4192)
                     /locus_tag="JN331_001570"
     CDS             complement(2672..4192)
                     /locus_tag="JN331_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7259744.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDDPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            4306..6570
                     /locus_tag="JN331_001571"
     CDS             4306..6570
                     /locus_tag="JN331_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY7259745.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDNILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LADFNRELRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSGQRV
                     EQSYPDLRSGERLIMIFALLAVVITAFAGWYIYRNLGSNLTAISRAMSRLAHGEADVT
                     FPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEALLHS
                     QKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNTNSPDAPRIQRAQQAAERGALLTQRL
                     LAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLTIEIEAQSPAWSAWIDVSQLENA
                     IINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGSGMSQAVKSQV
                     FEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAAKNL
                     SPAAVEQTAVNGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAASAE
                     IDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLRKPF
                     TRAQLAQVLGQIEG"
     gene            6651..7283
                     /locus_tag="JN331_001572"
     CDS             6651..7283
                     /locus_tag="JN331_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7259746.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIACESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            7352..7681
                     /locus_tag="JN331_001573"
     CDS             7352..7681
                     /locus_tag="JN331_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259747.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(7828..8586)
                     /gene="phnP"
                     /locus_tag="JN331_001574"
     CDS             complement(7828..8586)
                     /gene="phnP"
                     /locus_tag="JN331_001574"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAY7259748.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(8588..9022)
                     /gene="phnO"
                     /locus_tag="JN331_001575"
     CDS             complement(8588..9022)
                     /gene="phnO"
                     /locus_tag="JN331_001575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY7259749.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            complement(9009..9566)
                     /gene="phnN"
                     /locus_tag="JN331_001576"
     CDS             complement(9009..9566)
                     /gene="phnN"
                     /locus_tag="JN331_001576"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY7259750.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            complement(9566..10645)
                     /gene="phnM"
                     /locus_tag="JN331_001577"
     CDS             complement(9566..10645)
                     /gene="phnM"
                     /locus_tag="JN331_001577"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY7259751.1"
                     /translation="MTHLYAPGKGFSDVSFDLWPGEVLGIVGKSGSGKTTLLKSISAR
                     LTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSAGGNIGERLMA
                     TGARHYGDIRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLMDHSPGQRQFANREKYR
                     EYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAALCRARQIALASHDDATHAHV
                     AESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLL
                     DILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQNPAQALNLQDRGVIGEGKRA
                     DLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            complement(10672..11517)
                     /gene="phnJ"
                     /locus_tag="JN331_001578"
     CDS             complement(10672..11517)
                     /gene="phnJ"
                     /locus_tag="JN331_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY7259752.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(11510..12574)
                     /gene="phnI"
                     /locus_tag="JN331_001579"
     CDS             complement(11510..12574)
                     /gene="phnI"
                     /locus_tag="JN331_001579"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY7259753.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(12574..13158)
                     /gene="phnH"
                     /locus_tag="JN331_001580"
     CDS             complement(12574..13158)
                     /gene="phnH"
                     /locus_tag="JN331_001580"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY7259754.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLKALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(13155..13607)
                     /gene="phnG"
                     /locus_tag="JN331_001581"
     CDS             complement(13155..13607)
                     /gene="phnG"
                     /locus_tag="JN331_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY7259755.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(13608..14333)
                     /gene="phnF"
                     /locus_tag="JN331_001582"
     CDS             complement(13608..14333)
                     /gene="phnF"
                     /locus_tag="JN331_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY7259756.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(14354..15132)
                     /gene="phnE"
                     /locus_tag="JN331_001583"
                     /pseudo
     CDS             complement(14354..15132)
                     /gene="phnE"
                     /locus_tag="JN331_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
     gene            complement(15238..16254)
                     /gene="phnD"
                     /locus_tag="JN331_001584"
     CDS             complement(15238..16254)
                     /gene="phnD"
                     /locus_tag="JN331_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7259757.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(16279..17067)
                     /gene="phnC"
                     /locus_tag="JN331_001585"
     CDS             complement(16279..17067)
                     /gene="phnC"
                     /locus_tag="JN331_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY7259758.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALPGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(17200..17643)
                     /gene="yjdN"
                     /locus_tag="JN331_001586"
     CDS             complement(17200..17643)
                     /gene="yjdN"
                     /locus_tag="JN331_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY7259759.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(17901..18236)
                     /locus_tag="JN331_001587"
     CDS             complement(17901..18236)
                     /locus_tag="JN331_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY7259760.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            18637..20865
                     /gene="crfC"
                     /locus_tag="JN331_001588"
     CDS             18637..20865
                     /gene="crfC"
                     /locus_tag="JN331_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY7259761.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            20862..21740
                     /locus_tag="JN331_001589"
     CDS             20862..21740
                     /locus_tag="JN331_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259762.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            22004..23506
                     /gene="proP"
                     /locus_tag="JN331_001590"
     CDS             22004..23506
                     /gene="proP"
                     /locus_tag="JN331_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY7259763.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            23618..23707
                     /gene="pmrR"
                     /locus_tag="JN331_001591"
     CDS             23618..23707
                     /gene="pmrR"
                     /locus_tag="JN331_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY7259764.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(23683..24783)
                     /gene="pmrB"
                     /locus_tag="JN331_001592"
     CDS             complement(23683..24783)
                     /gene="pmrB"
                     /locus_tag="JN331_001592"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY7259765.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(24784..25452)
                     /gene="basR"
                     /locus_tag="JN331_001593"
     CDS             complement(24784..25452)
                     /gene="basR"
                     /locus_tag="JN331_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY7259766.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTMRGFGYMLVA
                     NEEN"
     gene            complement(25449..27092)
                     /gene="eptA"
                     /locus_tag="JN331_001594"
     CDS             complement(25449..27092)
                     /gene="eptA"
                     /locus_tag="JN331_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY7259767.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISIRLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(27196..>27598)
                     /locus_tag="JN331_001595"
     CDS             complement(27196..>27598)
                     /locus_tag="JN331_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7259768.1"
                     /translation="IFARVNKAGTPVAGLIIVGILMTIFQLSSISPNATKEFGLVSSV
                     SVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTTIAFLYCIWAVVGSGAKEVMWSF
                     VTLMVITAMYALNYNRLHKNPYPLDAPISKD"
BASE COUNT         5999 a         7388 c         7833 g         6378 t
ORIGIN      
        1 tggcggtatt cgttacacaa caccagctgg gccggttcgt cttcgtcaat caggttgtag
       61 cggtccagcg gttgtaaaaa gtggttatcg tggtcgtcgt cattgacaga agagacttcg
      121 ccaaccagaa catcgccaaa accggtttcc gcccaaaagc tgtgatacag gccgggcggc
      181 aggcagatac tttcgccggg ggacaggcgt aactgactgc ctgcgacatg agtctggcgg
      241 cagccgtcga tcaccaccgt aacgtcggta tcaactgttt gctcgttgct atcggcattc
      301 cacagttcaa caatcaaatt gccgccgccg cgattgatga tgtcctcacg cttacgccag
      361 tgaaaatgca ttggcgtgac ctgcgcatcg cgcacatgca tgatcttctc ggcgtaacat
      421 ttcacatacg gcattccttt tgctgagccg ttacgcaggg taaacagcgt cagcccctgt
      481 gctgcaaagt tgttaccacc aaaagcggta acatcccagc cgagtttgag atcaaaaacc
      541 tcctcccacg cggcggtgtc gagctgctgc cattgagcag gagagaaact ggcaaagggc
      601 ggcagatgta cgtcatgttg cgaaaagaat tgccgtgtat ggccgagaat gttgtttata
      661 gcggagcgtt tcatcgaaag cgttctcctt agcggtggcc gggcagcatc gccgcccggc
      721 gtgtggactt atttcaccgt ccagcctttg taattgccga cgttgttttt atcgatcatc
      781 gtcaccggga tcagcaccgg ctctttcggc gcaggtttgc cctgcaaaat gtcataaccg
      841 atctcaaccg ctttcgctgc catcacctgt ggatcttgcg ccggagtagc gacaaacaac
      901 gagttctcac gcttgagcgc ctcttcggcg tccgggcttc catccacgcc gacaataaag
      961 aactcgttac gttgcgcctg ttttgctgcc agatcggcac cgatcgctgt cggatcgtta
     1021 atcgcaaata cgccgtcgat cttcggattt gccgccagca gcccggtcat cacctccagc
     1081 ccgccctcac gactgccttt ggcgttcttg ttgtcagaga gcaccttgat gtcaggatgg
     1141 cgtttaaatt cggtctggca gccttcgacg cggttttgca cggcggacac cggcggtccg
     1201 ttgatgatca ccacgttgcc tttgcctttc aggcgatcgg tgatgtattt acaggccatt
     1261 tcgcctgcct gggtgttatc ggaagtgatg gtggcatcgg caccttctgc cgccacgtca
     1321 accgccacca ccacgatccc ggcgtctttt gcccgtttca ccgccgggcc gatccctttt
     1381 gaatcggcag cgttaaggat gatcatatcg acgttagcgg cgataaagtt gtctatctgc
     1441 gagacctgct gacccagatc gtagccgctg gaaaccagcg tcactttgac gttatcgccc
     1501 gccagtttgc gtgcttccag ctccgcgcct ttggtgatct gtacgaagaa cgggttcgcc
     1561 aggtcaccta ccgttacgcc tatcgatttc agatctttcg cctgggcgaa cggagctgcg
     1621 gccagcagcg cgcctgcaca gagcgcggtc actaagggct tcaatctcat gctgtattcc
     1681 tcatgtgtag ggtattgttg ttatgcactt tgatgatgtc gggtacggta tttgtcgata
     1741 agcaccgcaa tgatgatcac cgcccctttg atcaccagct gccagaagta agagacgccc
     1801 attaaggtca tgccattgtt gagcgtggcg ataattagcg cccccaccag cgtgccggtg
     1861 atggtgccga tgccgccgac aaaactggtg ccgccgagga tcaccgccgc aatggcatcc
     1921 agttcgtaac ccatacctaa gttgccgttg gcgctgtaca gccgcgaggt gctcataatc
     1981 ccgccaagcc ctgaaagcag gccgctcatg ccataaacaa acagcagcac cagccagacc
     2041 ttaatgccgg taagacgcgc cgcctgcata ttgccaccga cggcgtagat atgaacgcca
     2101 agcgtggtgc gacgcagaat aaaccagcac agcacgatca ccgccagcgc aatcaccacc
     2161 agccacggga ccggcccgag atagttattg cctatccact cgaaattaat gttggagttg
     2221 ataaccgtgg tgccatcagc cagcagatac gccgccccgc gcagcgccgt ataggtgcca
     2281 agcgtgacga taaacggcgg caatccggca aaggccacca gcgcgccgtt gaacagcccc
     2341 agcagcaggc cgagcatcag cgccgccggg atagacaaca tggcgaactc cgggctaagg
     2401 gagacaacca ttgccgccac cgccgtggtg ccgaggattg aacctacaga aagatcgatc
     2461 ccaccggtca aaatgatgaa ggtcatcccc gccgccagca caatgttgat cgacgcctgg
     2521 cgggtaatat ttagcaggtt gctttcggta aaaaagtttg gcgcgataaa gccaaatacc
     2581 gctacgatca ggatcaaaat gggcaaaatg ccgaccgttt gcatcagatc gctcatcagc
     2641 atttttttgg cagaggtttt tgcagaggtc gtcatgaggt caccgcctgg gaatgggcgt
     2701 cgttgacgcc agtcgctaag gtcataatgt tttcctgggt gatgtttttg ccatttagct
     2761 caccggcaat gctgccttcg cgcatcacat ggacgcgatc gctcattccc actatctccg
     2821 gcagttcgct ggagatcatc aggatcgcca cgcccttgcg cgccatctcg ttcatgatcc
     2881 ggtagatctc actttttgcg ccaacgtcca cgccgcgggt gggatcatcg agcagtaaaa
     2941 tgcgcgggcc aatcgccacc cagcgggaga tcaacagttt ttgctgattg ccgccggaaa
     3001 gcccgcccgc gcgtacctgg gcatgaggca cgcgaatgtt gagcaactta atggcgtcat
     3061 cggaaatggt ttgcgctttt ttgcgattga gcattcccca gctggcatcg cgctccaggg
     3121 ttgccatagt gatgttttcg actgccgcca tttcaaggaa taagccttgt tctttgcggt
     3181 tttcggtgag aaaaccaatg ccaagatcga tggcttcgcg cggggagtgg atcaccaccg
     3241 gttcgccatc gacttcaatc attccgcctg ttgctttccg cacgccgaaa attaactgcg
     3301 ccagttcgga acgtccggct cccaccagcc cggcgaggcc aacaatttcg ccggaaagca
     3361 cctgcaggca gctcggctgg actttgccgc cgtcggtcag gtggtgaaca ttcaggcggg
     3421 ctttaccgag cgggatatcg cgctctttat tgaacagatc gctcagtggc ctgccgacca
     3481 tcatccgcac cagctccccg gcattgaggt tatcgcgggt caggctgcca acgtattgcc
     3541 cgtcgcgcag cacgctgacg cgatcggaaa gctcatacac ttccgccata cggtggctga
     3601 tgtagataat cgccatccct tcatcgcgca gccgcataat cagttcaaaa agacggtgag
     3661 tttcacggga ggagagggca gcggtgggtt cgtccatcac cagaatgcgg ctgttgcgat
     3721 gtagcgcgcg ggcgatttcg acctgctgtt gttcggcaat ggtcagcgtc atgaccttat
     3781 cgctggcgtt aaactgcgcg ccgaggcggt cgatcactct ttgcgcctgc accagcatct
     3841 cttttcgctg caccagcccg ccgtgggaaa gctcgctgcc gaggaaaata ttttctgcca
     3901 ccgtcaaatt gggtgccagc tgcatctcct gataaatcag cgtaataccg gcgcttaagg
     3961 catcttttgg cgtgcgaatg tgaaagggct tgccgtctat caggatctcg ccgctggtgg
     4021 cggtatacgc ccccgccagc accttcatca gcgtgctttt tcccgcgccg ttttccccca
     4081 tcagggcatg gatctcgcca gggtagaccg tcaaatccac ccctttgagc gcataaaatt
     4141 tgccgaaggc tttggcaata ttgcgcatct caagaaccgg cgtgctgttc atggtggact
     4201 cctgcgaggt tgtcgatatc agcactccat cacaggaaag taaatgcaaa atgtcagaag
     4261 ccgttcatat ttgtcatatt gcgatttttc ttcacggagc gtgatatggc gcaccccgga
     4321 cgtcactttt ttgccagcgc gcgcgggcga ttgctgcttt tgaatttgct ggtggtggcg
     4381 gtgacgttga tggtcagcgg cgtggcggta atgggctttc gtcatgccag ccagatgcaa
     4441 gagctggtgc agcagcaaac tgtagatgat atgaccggca gcctgaatct ggcgcgcgac
     4501 acggcaaacg tggcaacggc ggcagtgcga ttgtcgcagg tggtgggagc actggaatat
     4561 aaaggcgaag ccgagcggtt gcaggagaca cagcgggcat taaaaagctc gcttgcacaa
     4621 ctggcgaacg cgcctttagc gcagcaggaa gccgggctgg tgacgcgcat catcacgcgt
     4681 agcaacgaac tgcaaaccag cgtcggcgga atgctggaac gcgggcaaag gcggcatctg
     4741 gagcgtaacg cgctgttaag ttcgctgtat caaaacttaa gttatctgcg gcatctgcaa
     4801 aaagtcactc acgcgcaaga taatattttg cttaacgaga tgaaccgact gattgttgcc
     4861 gccattgcca cccccgcgcc gcaggcgatt attcatcaac tggtcggagt gatgtctgcc
     4921 ttgccaactc acagcgatac gccattggtt aatacgctac tcgctgattt taatcgtgaa
     4981 ctgcgtaaac tcgcgccact ttcggcggct ctggagcaaa gcgatctggc aatcagctgg
     5041 tatatgtttc acatcaaagc gctggtggcg atccttaatg gcgacatcaa ccgttacgcc
     5101 gaacaggtgg cgacgctctc tggtcagcgg gtggagcaaa gttacccgga tttgcgctcc
     5161 ggcgagaggc tgatcatgat tttcgccctg ttagcggtgg tgatcaccgc gtttgcaggt
     5221 tggtatatct atcgcaatct cggctctaac ctgacggcga tttcccgcgc gatgagccgc
     5281 ctggcgcacg gcgaagcgga cgtcactttt ccggcgttac agcggcgcga tgagttgggt
     5341 gaactggcgc gcgcctttaa cgtttttgcc cgcaataccg catcacttga acgaacgtca
     5401 cggctgttga aagagaaaac cacgcagatg gagattgccc aaaccgagcg gcaggggctg
     5461 gaagaggcgc tattgcacag ccagaagtta aaagcggtcg ggcaactgac gggcgggctg
     5521 gcccatgatt ttaataatct gctggcggta attattggta gcctggaact ggtgaacacg
     5581 aactcacccg acgcgccgcg aatacagcgg gcgcagcaag ccgccgaacg tggcgcgcta
     5641 ctgacccagc gattgttggc attttcccgc aagcagtcct tacatccgca agccgttgaa
     5701 ctaaaaacac tgctggaaga gttaagcgaa ttaatgcgcc actccttacc ggcgacgctg
     5761 acgattgaga ttgaagcgca gtctccggcg tggtccgcgt ggattgacgt cagccaactg
     5821 gaaaacgcca tcatcaacct ggtgatgaac gcccgcgacg cgatggaagg gcagacggga
     5881 attatcaaaa ttcgtacctg gaatcagcgt gttacccgca gcagtgggca gcgtcaggac
     5941 atggtggcac tggaggtcat cgatcatggc agcggtatgt cgcaggcggt gaaaagccag
     6001 gtgttcgagc cgttctttac caccaaacag acggggagcg gcagcgggct gggcttgtcg
     6061 atggtctatg gtttcgtgcg ccagtccggc gggcgcgtgg aaattgaaag cgcgccgggg
     6121 caggggacaa cggtcagatt acagcttccc cgcgcgctga cggcggcgaa aaacctatcg
     6181 ccagcggcgg tagaacagac agctgttaat ggcgataaac tggtgttagt gctggaagat
     6241 gaagccgcgg tgcgccagac catctgcgag cagttgcacc tgctgggcta tttgacactg
     6301 gaagcgagca gcggcgaaca ggcgctggac ctgctggcgg catcagcaga aatcgatatc
     6361 tttatcagcg acttaatgct acctggcggc atgagcggcg cagaagtggt caatgcggcc
     6421 cgcaaacttt acccgcacct gacgctgcta ctcatcagcg gtcaggattt acgccccagc
     6481 cataaccccg cgctgccgga cgtggcgctg ctgcgcaaac cctttacccg cgcgcagctg
     6541 gcgcaggtgc tggggcaaat tgagggatga ttttcagaat ttatcaagag gctcgaagca
     6601 aacgtgctgc tattgtgagc aattctgttc tgaattgaag tgatttacat atgatcgtga
     6661 tgccaaccac ctatagccct atgacggttg ctcgttcttt tcgcgtggtg gaagagatcg
     6721 agctttctgg acgtctgtta caggtaattt atgacaacca gacgccaaga gcggccatca
     6781 ttgaagcgga cattgaagtt tttgcaggcg tgccgcgtca tcgggtactt gccgctctcg
     6841 atctgcagcg taaaaaagag ttcggcagcg atatcattgc cgttgaacgc tggtgggaag
     6901 actcggatct ggtacaggtg gaaggtattt gtgtctctcc aggtgagcgc gataagggtc
     6961 tggcaactcg gctgtatgaa gcgctaatcc tgcaatgcgg tatcacgcta atcagcgatt
     7021 ttgatcaata tgatggcggg aagatgctct ggcagaagat cgcgtgcgag tccgatcaga
     7081 tttccgtgtt tgtattagac actgaacaag gggcattttg gccctacgat ggcacgaaag
     7141 tgatctacga tggcggctgc attccggaag agcgtatctg gagtatttca ccggatcaaa
     7201 aatgtcatgg cattgtattg gtggcggaaa ataaacagcg agctaaccag ctaatggaag
     7261 agtctgcgcc ttaccgaatt tgagattact ccgctactcc ggtcattcac actcaactaa
     7321 cgttaagcca gtccatcgca gagactggct tatgaaacct tactccacgc tttttctttt
     7381 caccctgctc acgctgtcca ccgttccggc ccaggccgat attatcgacg atactattgg
     7441 taatattcag caggcgatta atgacgctta taactctgac cgtgggcgag actatgaaga
     7501 ttcgcgcgac gacggctggc agcgtgaagt gagcgacgat cggcgcagac aatatgacga
     7561 ccgccgccgc cagtttgaag atcgccgcag gcagctggac gatcgccagc gccagcttga
     7621 tcaggagcgt cggcagctag aagatgaaga gcggagaatg gaagatgagt atgggcgatg
     7681 aggttgggta tggagcaggc atcgcccctc accccaatgg ggagagggag aaaaccagcg
     7741 caatattcaa tatcgagcac aatcgctccc ctcgcccctc tggggagagg gttagggtga
     7801 gggggccagc gcgcacattc cctctcatca cgccacccca atctccatcc catcaaaccc
     7861 cgcctcaaac cccgacggca gtgcgttttc tatcagccac gcatcaaact ggtggctgat
     7921 atgggtcaga atcacccgtg gcgagcggat aacctgattc agcgcaagca cggtatttaa
     7981 atcacagtga ttacgcggcg catccgcgcg cggcgggtgg ctgcaatcaa tcaccattac
     8041 ctgtggctga ttattgcgta aaaatttcag cgttttttcc ggcaggccag cggtgtcaga
     8101 tagccacgcc acccggctat gtgccgtttc cagcagataa ccgaaggtca gttttgagtg
     8161 gttaagcggc agcggcgtga cctgcaaccc ctgcaaatca aacaccacaa acggttccac
     8221 tgtgtggctg aaatcaagca ggcccggatg tttaaacaga tcgtcgcaac cctggtcatc
     8281 cggcgggccg tagatcggga tcggatcgcc cacgccccag cgcagcggaa acagcccctg
     8341 ggcgtgatcc atatgataat gcgtcagcaa aaactgctgg aacgatccgg gcgaccagcg
     8401 atcggcgaga tcgtgcagcc cggcgtcgat cagggtgatt gcgtcgttaa acttcactac
     8461 gccgctgcat ggctggcggc gatactgcgg tgagcgccgc gctcggtcgc aggccgcgca
     8521 ctcgcagccc catgccggaa cgccctgtgc gccgccagtg ccggtgagcg tgagggtcag
     8581 gctcatgtta cagcaccttg gtgaagcgga agtgactctg ctcgtagcct tctcgcagat
     8641 agaaacggtg cgcgtcgtgg cgtttcacgt tggtcgaaag cgcggtcatt tcggccccgg
     8701 cctggcgggc ttcttcttct gcccatgcca gtaatttact gccgacgttc agaccgcgcg
     8761 cctgcggcat caccaccagt tcctgaattt cgccgatcca gttgacatga tgcagatgaa
     8821 actgcaaatg caggccgatc atgctgacaa cttcgccatc cagcagcgcc agatggtagc
     8881 gcatgtttgg gtcgcgcagg ttggcgttaa agcccacgcg aaacgcatgg tggtcaaact
     8941 ccgcctgttt tagctcacaa atcagcgcgt aaactgcgtc ggtgtcgtac tgcgtagccg
     9001 ggcgaagctc acaagcaggc atggtgtttc tccttctgat gaatcagcgt cagcagcttg
     9061 tcgaccgact ggcgcaggct gccgtcatta ttgagcgtaa gacaatcctg aggagtatag
     9121 cgggcagcgc gtgctagtcg ggcgttaatt tcactggcat tttcacgacc acggttttcg
     9181 aggcgctggc gcaggatctc cggcgaaacc tgtaagcaaa tgggcagcag cgccgattga
     9241 tagcgcgccc gcgcctgcgg cagatgggcg cgtgagccgt tgaccagcac gtcgaatccg
     9301 gcgtgcagcc agagatcaat ctcaacgccg acgccataat acagactgtt agcgtgccag
     9361 cttaaggcca acagattttg ccccgcgcgg gtaaaaaact cctgctcgct cagggcgata
     9421 tggttttcac ttccggcgct ggcgtcgcgc gtaatgtagc gatgcgccac cagtaactga
     9481 gtttgttccc gcaggcggat ttccgccagc aggctgtctt tcccggagcc ggacggcccc
     9541 attaaccaaa tcagttttcc catcatcaga acaccctttt accctgacgc cagacgtggt
     9601 cgatatgaat atgattgtcc ttgcgatgcg ccagcaccag gtccgcgcgt ttgccctcgc
     9661 caatcacccc gcgatcctga agattaagcg cctgcgccgg attttgggtc actaacttca
     9721 ccgcctgcgg cagcgtaaag cggttgctct ggtcatcggc gacgcgaaat gccgcatcca
     9781 gcaggctggc ggggtagtag tcggaagaga ggatatccag caggccaagc tgcgccagtt
     9841 cactggccgc cacgttgccg gagtgcgaac cgccgcgcac gatattcggc gcgcccatca
     9901 gtacgttcat gccatgcttg cgcgaggctt ccgccgcttc aaacgtggtg ggaaattcgg
     9961 cgatcacgct gccaagcagg tgggattcgg caacgtgggc gtgggtggcg tcatcatggc
    10021 tggcgagcgc aatttgtcgc gcgcggcaca gggcggcgat ggattcgcga ttcggctgcg
    10081 accagcgtgc ggcaagcgcc agttgctctt cttcgtactg ctgcatctgc gcatcggtga
    10141 gcgagtattt gccctgataa tattcgcgat acttctcgcg gttggcgaac tggcgctggc
    10201 ccggcgagtg gtccatcagc gtcaccggct cgcgctgcac cagtttttca aacagcggca
    10261 gcgtggtgtg atgcggcagt tcgcagcgca ggtgcagacg gtgctcggcg cggatgtcgc
    10321 cgtaatgacg ggccccggtc gccatcagcc gctcgccgat attgccgcct gccgagacct
    10381 ggcgacgcag gccgtcgagt ggatgctgat gcaccacgcc ccattcggtg cgcagcaggc
    10441 gacggcggtc ggcctcgctc atcgcataca acgaacgatt ctcgtagcga atctccccct
    10501 gctgcggcgt caggcgcgcg gagattgatt tcagcagcgt ggtcttcccg gagccggatt
    10561 tcccgacgat gcccagcact tcccccggcc ataaatcaaa agagacatcg ctaaagcctt
    10621 tgcccggcgc gtaaaggtgg gtcaggttat tgaccgaaag taacggttga ttcattggtt
    10681 ttttgcctcg ctctgttggc ggcaataatc ggtatcggag cagacaaaca tgcggtttcc
    10741 ggcgtcatcc agcaccactt cgtcgagata gctgtgggtc gatccgcaga tggcgcatgg
    10801 ctcatcccac tgctgaacgg tgaacgggtg atcgtcgaaa tcgagacttt ccacgcgggt
    10861 aaacggcggc accgcataga tgcgcttctc gcgccctgcg ccgaacagct gcagggcggg
    10921 catcatgtcc attttcgggt tatcgaattt cgggatcggc gacgggtcca tcacgtagcg
    10981 cccgttcacc ttcaccggat aagcataggt ggtggcgata tgaccgaagc gggcgatatc
    11041 ttcatacagt ttcacctgca tcacgccgta ctcctccagc gcgtgcatag tgcgggtttc
    11101 cgtttcgcgc ggctcgataa agcgcagcgg ttcggggatc ggcacctgga aaataactat
    11161 ctgatcttcg gtcagcggcg tttcggggat gcgatggcgc gtctggataa gcgtcgcatc
    11221 gtccgtacgt tcagtggtgt tcaccccggt cacgcgcttg aagaagttgc gaatcgacac
    11281 ggcgttagtg gtgtcgtctg cgccctggtc aatcaccttc aacacgtcgc tttcgccaat
    11341 cacgctggcg gtgagctgaa tgccgccggt tccccagccg tagggcatcg gcatctcgcg
    11401 cccgccaaac ggcacctgat aaccggggat agccaccgct tttaagatgg cgcggcggat
    11461 catgcgtttg gtctgctcgt cgaggtaggc aaagttgtag ccgctcagat tagccatggt
    11521 tctgctcctg ttgcagacgt ttgagtagct ccagttcggc ctggaaatcg acgtagtggg
    11581 ggagtttgag gtgcgagaca aagcctgcgg cttcgacgtt gtcggcatgc gccagcacga
    11641 actcttcatc ctgcgccggg cctgtcgcgt gctcgccgta ttccggggct tgcaacgcac
    11701 ggtcaaccag cgccattgcc atcgctttgc gctcgctcat gccgaatacc agcccgtagc
    11761 cgcgcgtgaa gtgcggcggc tcatccggcg ggtcgataaa accgttgacc atttcacact
    11821 cggtcatcag cagttcgccg acgtttaccg caaatcccag ctcttccggc acaatcgaca
    11881 cgtcgatgta accactgcgg atctcgcccg cgaacgggtg attgcgtccg taaccacgct
    11941 gggtggagta ggccagcgcc agcaaatagc cttcgtcccc gcgcattaat tgttgcaggc
    12001 gggaggagcg tgagcagggg taaaccggcg gcgtgcgggt gatgtcatcc ggctgtgcgc
    12061 cgctatcttc ttcaaacttc gccagcccct gacgcgccag caggctgaaa acgtgcggcg
    12121 acggctgttg ttcgctgtcg gcggtggtca gcgtcggcgc ttcaccgttt gccagcaggg
    12181 tgaaatcgag caggcgatgg gtgtagtcgt aggttgggcc aagcagctgg ccgccgggaa
    12241 tgtctttata aaccgccgaa atacgccgtt cgagacgcat ctcggtggtg tcgagcggct
    12301 cgcttaccgc cagtttcgcc agcgtggtgc ggtaggcgcg cagcaggaaa attgcttcaa
    12361 cgttatcgcc gctggcctgt ttcagcgcca gcgccgccag ttcgcggtcg gcaacgccgc
    12421 cttcggtcat cacgcgatcc accgcgagat taagctgctg ttcaatttgg gcgacgctca
    12481 gttcgggcaa atcggtatcg ccccggcgtc ggctctcttg cagggcgtgg gcggcgtcga
    12541 tcgccttttc gccccctttc acggcaacgt acatcagcac acctccacat gagtggttcg
    12601 cggaatagcc agcaggcgtt cgccgcaggt caggatcagg tcgatgccga gcgggaacgg
    12661 gtgcgggcgc tcggtgagtt cgtgcagaat gcactccggc agctgcggag cgatcattcg
    12721 ttcttcggca atacccgcgc cggtgagacg caacatgcgc ccgccgctca ggctggcgac
    12781 ctgtaaaatc agcgtcgcgc ccgcttccgg cgcaacggcg gtgccggtgg aaagggcttt
    12841 gagctgtttg ctggaaattc cgtcatccgt caccgcgaag gtcgcctgtt ccggctggct
    12901 gaccagcggc gcgttggtat gaaaacgcag gctctgattg acgatatcgt tactcaatgg
    12961 cgcggcaagc cacaccggcg tgtcgttatc agccagcgtc agcagcacgc tggtggtggc
    13021 gatattcagc ggctgccagc cgcgcttgag ctgatgcagg gcgacaatca cgcccggctc
    13081 gcttatggcc tttaacaggc gacgaaaact gtgctgggca tcctgcacgg gaagcataaa
    13141 agcggtttcc agggtcatgc gttgtctccg cgaaccatcg taaagaagtc gacccggctg
    13201 gcgttcactt cggcctggcg tgcggcaatg cgtgccatac ggtcagcgtc cagcggggca
    13261 ataagggttt ctgataagtt ttgaaagtgg cgagattgct gcatcagcgc atcaatcagc
    13321 gcgcagcgtt cggtgtgctg tttatcacgc cccagcaccc agctgtagcc gagggtgccg
    13381 tcagtcaggc gtacggcggc gcgggtcagc gtggcgtcgc cggcaaaaaa gcgttcgccg
    13441 gtgccgccca ttcgcgcctg aatctgtacc aggccggttt cggcggtgcg gatcacttca
    13501 tagtcggcgg tgatgttcag cgcgttcagg cgggctgcca gttcagccgg ttggctgtgc
    13561 gccagtacgg acatccagtg ctggcgggtc gcggtatctg cgtgcattca gtgctccata
    13621 gtgaattcaa tcatgtcggc gcgcgtcagg ctgacggagt actccgccgg gctgctttca
    13681 ccgtcacggt ggttaagggt gcgcacgcac agcagcggcg acatattcgg gatttcaagg
    13741 cgctggcact ctttggcctg ggcgcggcgg gcgctgatcc gcgtctggct gcggcgcagc
    13801 gcaattccgg tttgctcgcg cagaaaatcg tgcagcgaac cgctgtcgaa gcgttgcagc
    13861 gtcggccaaa gggtgaggtc cgcaaagtag tggtcgatta aacagagcgc gacgccgttg
    13921 acccggcgca gggtgcgcaa atggataacg ttctcgccct cggtaatccc cagcgcgtca
    13981 gcaacgtgac cggacgcggg gcgcaatacc gacagcagtt tttcgctggt gggatggctg
    14041 ccctggtcca gcaggttctg gctaaaacgc gcctgggcgt tgagcgggta atcgaacggg
    14101 cgcatcagca ccagcacgcc gacgccctga cggcgctgta cccagccttt ttccaccagt
    14161 tggtcgatgg cgcggcgcag ggtgtggcgg ttcacctcaa agcgcgccgc cagttgctgc
    14221 tcggcgggaa gatagtcgcc gcagcggtag tgttgacgaa gctcctgctc aagttttgcg
    14281 gctatctcct gatagcgtgt tgggtagctg gtcggatgtg tagacaagtg catagatatc
    14341 aatgcctcgc ttatcagata aagtgcttac gcaaccgttg agagaggaaa tccagcaggc
    14401 tgaccgtgac gatgataagc accatcaggg cgcaggtttg ctggaactgg aaaccgcgaa
    14461 tcgcttccca cagggtgacg ccgatcccgc ccgcgccgac catgccgacg acggtcgccg
    14521 agcggacgtt ggattcgaag cgatgaggga gtaggagatc agcagcggca tcacctgcgg
    14581 cagcacgccg taaaggatct cttcgagttt gttggcgccg gtggcgcgaa tgccttccac
    14641 cgggccgggt tcgatcgctt ccaccgcttc ggaaagcagc ttggagagca cgccggtggt
    14701 gtggataaac agcgccagca cgccagcgaa cgggccgagg ccgacggcga ccacgaacag
    14761 catggcgaag accatttcgt taatggcgcg gcaggcgtcc atcaggcggc gaacgggctg
    14821 gtaaacccac cacggcacca agttttcggc gctcatcaga ccgaagggga tggagagaac
    14881 cactgccagc gcggtgcccc agacggcgat ttgcagcgtg acggccattt cggtgaggta
    14941 atcctgccac tggctgaaat cgggcgggaa gaagtcggca gcgaaggttg ccatgttgcc
    15001 gccgtcttta atcagcgtaa gcggggccat ttccgcgccc tgccacgaga cgaccagcac
    15061 ggcgagaaca acggcccagc tcagaagcga gaaccagcta cgcttgggtg gggcgatggt
    15121 gatggtttgc atgtttggct ccggtctgta ggccggataa ggtgcttgca ccgcatccgg
    15181 catcaacgcc tgcacattgt cggatgcgac gccggggcgt cttatccgac ctacgattta
    15241 ctgcaccgct ttactcaccg aactcatcgc gcttaacgcg ttgttcaggc ggtccaggtc
    15301 atccagctgc gcctgaatct cggtggtttt cgccagcttg tcctgctcat tcagcccttt
    15361 attgcctttc acgctttgta tctctttaaa cagtgcgagc tggcgaatcg gcaccagttg
    15421 caggtcgctg gaagcgcgga acggcgccca gcccaggcgt tccagcaccg ctttctcttc
    15481 cggcgttttt ccgtagttca taaagaagtc gtagatcttg tctttggtag tttcggacag
    15541 attcttgcgc cagacgatcg gatcgcctag gatcagcggc gatttccaga tcaccttcag
    15601 ctctttaagc ttctccggcg cggaggtttt cagcttgtcg aggttttcgg tgttgttggt
    15661 ggcaacatcc acctgcttgt tggcgacggc cagcgcgttg gtttcatgcc cggcgttgac
    15721 ggtgcgcttg aagtcgctgg cggagatatt gtttttagcg aagacgtagt aaccggggac
    15781 gaggaagcca gaggtggagt taggatcgcc attgccgaag gtgagatctt tccgcttcgc
    15841 cagcagatcg ttcaggttgt tgatcgggct gtctttgttg acgatcaaca cgctccagta
    15901 acccggcgat ccatccgccg cgaccgtctg ggcgaagacc tggccattgg cgcgatccac
    15961 cgcttccatc gccgacaggt tgccgtacca ggcaatatcc actttattga agcgcatccc
    16021 ctggataatg cccgcgtagt ccggggcaaa gaaggcgttg accttcacgc ccagtttctt
    16081 ctccatatcc tgtaaaaacg gcgtccattg cggtttcagg ttttgctgtg attccgttga
    16141 aataatgccg aaattcagcg ccttttcctg ctcttcggcg tgtgccgggg ttaacagggt
    16201 gctgaggctg aacatgctgg tgaaggccag cgaggcaatt atcttagcgt tcattcgttt
    16261 tcctcaatga tggggatatc aggcagcttt cgcgttctct tcgatgcggt taatgctgcg
    16321 gtagagatgg tcaaaacgtt cgttatcaaa cagttggctg ctgccgtcgt agaagacgtg
    16381 cccctgacgc agggcgacga tgcgttcgca gtagcgcagg gcgtaatcca cctgatgcag
    16441 cgtgacgacc acggtgatgc cgtcgttctg gttgatgtcg cgcagggtgt ccatcacgat
    16501 gcgcgctgat tccgggtcca gcgaggcgat gggttcatcg gccagaatca ctttcgcctg
    16561 ctgcatcagc gcacgggcaa tcgccacacg ttgctgctgg ccgccggaga gggtggaaac
    16621 gcgctgatgg gcaaaatgca ccatgccaac gcgggtcagc gcctgtaacg cgcgctgttt
    16681 ctgctcgccg gtgaaccagc taaaacaggt gcgccagaac ggcgtgctgc cgagcgcgcc
    16741 aatcagcacg ttctccagta cgctcaggcg gttcaccagg ttgaattgtt gaaagatgta
    16801 gccggtgttg gcgcggcttt tgcggatatc gcgggccagg cggccttcgc gctggacagt
    16861 gcggcccagt agctcgatat ggctaccggc ggatttatcg ccggtaatca aaccgcttaa
    16921 atgacgtaaa agggtggatt tgccggaacc cgacggccca ggcagagcca ccatttcact
    16981 gtgatgaatg ttcagatcaa ccgcatgcag cgcttgatgc tgattgaagg ttttggcgag
    17041 cttctcgaca cggataatcg tttgcatgat gcagcctccc taaaaaagtg gctccatcgt
    17101 ggcggattat tgtgacgatt tggttaatta aaagtgacta acagatgaag agttaacggg
    17161 aattcgatga cagtgcggga gggcgggcct cccggcgggt tattgcgttg gttgttgttt
    17221 gacgacatta atcatccacg gtacgccaaa tttatcggtg actttgccaa agccatgcgc
    17281 ccagaaagtt tcctgccagg ccatttcgat ttttccgtta gcggcaaggt tatcaaacca
    17341 gcgttttcct tcttcgacct gttgcgaatc gagcaccagc gtaaagccgg agtagctggc
    17401 ttttcctgac ggtatagcat cgctcatcat gatgtcgctt ccggcaatgc gcacgttggc
    17461 atgagcgatg gcggtatcgg gaaattgcat tccggaaggg cagttctcgg cgctgtcctg
    17521 cgctgatttc ggcatttcgc cgaagctgat tttatagagc agttccgcgc ccaacgtacg
    17581 ttgataatag gcaatcgcgt cggaacagtt accggcaaaa gagaggtagg gacttaacgg
    17641 catgatggtg acctcagtta agagaagcca gttaagtgta gttcgaaatt tatacagatg
    17701 agaggctgcc tgataagacg cgcaagcgtc gcatcaggca gtcggcactg ttgccggatg
    17761 cggcgtaaac gccttatccg gcctacggag ggtgcgggaa tttgtaggcc tgataagacg
    17821 cgcaagcgtc gcatcaggca gtcggcatcg ttgccggatg cggcgcgagc gccttatccg
    17881 gcttatcgat cacaatacaa tcagttcttt ttcacaaact cagatttcag tttcatcgga
    17941 ccaaaaccgt cgattttgca atcgatgtta tggtcgcctt caaccaggcg gatgtttttc
    18001 actttggtgc caattttcag catcgaagag ctacctttca ccttcagatc tttaatgatg
    18061 gtaacgctgt cgccgtcagc cagcagattg ccgttagcat ctttaacgat cagctcgtcg
    18121 ctttcctgtg caggttctgc gtcgttccat tcgtaggcac attccgggca gatgtacatg
    18181 ccgttatctt cgtaagtgta ttcggagttg cattttgggc agtgtggtaa tgacatgtgg
    18241 atttcctcaa aagtcagcag ggcaaaagcg ccaaaaaacg gcagattgcc gaaaaaggcc
    18301 gcaattatac acaaaatccc tgagtttgtc gggggtactg ctgattttag acgacgatcg
    18361 ttttcatttg aaaggaaacg caaaaagaaa ataatctttt tgcctctatt tattatttaa
    18421 gataatgtta atgctctact tgttcttttt tttattcaca tgatattttt atctttctga
    18481 cgttttaaac gcatcagaag acgttattta ttctgataaa aaaaacacac taaatataaa
    18541 attatttctt gcagtaatta taaaagccga aaacagaact caaaaacaat ctggctaaat
    18601 aaaagtaaca aaatttgctt taaggaagaa ttttctatgt acacacagac cctgtatgag
    18661 ttaagtcagg aggctgaacg cctgttacag ctttctcgcc aacagttgca gttactggaa
    18721 aaaatgcctc tctctgtacc cggagacgac gcgccacaac tggctttacc ctggagtcag
    18781 cctaatatcg ccgaacgtca cgcgatgctg aataatgagt tgcgtaaaat ttcccgactg
    18841 gaaatggtgc tggcaattgt cggtaccatg aaagcaggga aatcaaccac cattaatgcc
    18901 attgttggta cggaggttct gcctaatcgt aatcgcccaa tgactgcgct gccgacgctt
    18961 attcgccata cgcccgggca aaaggaaccg gtactgcatt tttcacatgt cgcgccaatc
    19021 gattgtttaa ttcaacaatt acaacagcgc ctgcgtgatt gcgatattaa gcatctgacc
    19081 gatgtgctgg aaatagataa agatatgcgt gcgcttatgc agcggatcga aaatggcgtc
    19141 gctttcgaaa aatattatct gggtgcccag cctatttttc attgtctgaa aagtttgaat
    19201 gatttagtgc gactggcgaa ggcgctggac gtcgattttc ctttttctgc ttacgccgcc
    19261 attgagcata ttcccgtgat tgaagtggag tttgtccatc tggcggggct ggaaagttat
    19321 cccggtcagt tgacgttact ggataccccc gggccaaatg aagccgggca accgcatctg
    19381 caaaaaatgc ttaaccagca gctggcacgc gcctcggcgg tactggcggt gctggattat
    19441 acgcaactga aatcgatctc cgatgaagag gtccgtgagg cgattttggc ggtggggcaa
    19501 tcggtgccgc tgtatgtgct ggtcaataag ttcgatcaac aggatcgtaa cagtgacgac
    19561 gccgaccagg tgcgggcact gatttccggg acgctgatga aaggctgtat tacgccacag
    19621 cagatattcc cggtgtcgtc gatgtggggc tacctggcga atcgggcgcg ctatgagtta
    19681 gccaacaacg gtaagttacc agcgccagag caacaacgct gggtggaaga ttttgcccat
    19741 gccgcgctcg gcaggcgctg gcgtcatgcc gatctggcgg acctcgaaca tattcgtcat
    19801 gctgccgatc agttgtggga agattcgctg ttcgcccagc caattcaggc gttgcttcat
    19861 gccgcttacg ctaacgcctc attgtatgct ctgcgatctg ctgcgcataa actgttgaat
    19921 tacgcgcagc aggcgcggga atacctggat tttcgtgcgc acgggttaaa cgtcgcttgt
    19981 gaacaattgc ggcaaaatat ccatcaggtc gaagaaagtt tgcagctatt gcaactcaat
    20041 caggcgcagg tgagcggcga gattaaacat gaaatcgagc tggccctgac ctccgccaac
    20101 cactttctgc gtcaacagca agatgcggtg aatgcgcagt tagccgcctt gtttcaggat
    20161 gattcggagc cgttaagcga gattcgtacc cgctgtgaga cactgttaca gacggcgcag
    20221 aacaccatca gtcgcgactt tacgctgcgt tttgccgagc tggaatccac cctttgccgg
    20281 gtgttaaccg atgttattcg ccccattgag caacaagtca aaatggaatt gagcgagtca
    20341 gggtttcgtc ctgggtttca ttttcctgtt tttcacggcg tagttcccca cttcaacact
    20401 cgccagctgt tcagtgaagt catttcgcgc caggaagcaa cggacgagca gagcacgcgt
    20461 ttaggcgttg tgcgtgagac tttttcgcgc tggttgaatc agcccgactg gggacgggga
    20521 aatgagaaat ccccgacaga aacggttgat tacagtgtgt tgcaacgagc attaagcgca
    20581 gaagtcgatc tctattgcca acaaatggct aaagttctgg cagagcaggt cgatgaatct
    20641 gttacggcag gcatgaatac ttttttcgct gagttcgctt catgtttgac agaattacag
    20701 acgcgtttac gcgaaagtct ggctctgcgt caacaaaatg aatcggtggt caggctgatg
    20761 cagcagcaat tgcagcagac tgtgatgact cacagctgga tttacaccga cgctcagctg
    20821 ttacgcgatg atattcaaac acttttcacg gcagaacgat attgaccaag acgttactgg
    20881 acggccccgg tcgcgtgctg gagtcggttc atccgcgctt tttagtggat ctggcgcagg
    20941 gcgatgatgc ccgccatcct caagcccatc agcagcagtt tcgtgaacga ctgatgcagg
    21001 aacttctttc gcgtgtgcag cttcagacat ggacgaacgg cggcatgtta aatgcgccgc
    21061 ttagcctgcg actgacaatg gtggaaaaac tggcgtcgat gctggatccc ggtcatctgg
    21121 cgctgacgca gatcgcgcag catctggcgc tgctgcaaaa aatggatcac cgccagcact
    21181 ctgctttccc ggagctcccc cagcaaattg ccgccttgta tgagtggttt tcagcccgtt
    21241 gtcgctggaa ggaaaaggcg ttaacgcaac gaggcctact ggtgcaggca ggtgatcaga
    21301 gcgagcaaat ttttacccgc tggcgtgctg gggcgtataa cgcctggtcg ttgcctgggc
    21361 gctgttttat cgttctggag gagttgcgct ggggtgcatt tggcgatgcc tgccgtctgg
    21421 gaagcccgca agcggtggtg ttgttgctgg gtgatttgcg cgagaaagcg acacaacatc
    21481 tggcagagag tatcaatgcg gcaccgacca cgcgtcacta ttaccatcag tggtttgcct
    21541 cttcgaccgt tccgacgggc ggggatcatg ctgatttttt aagttggctg ggaaagtgga
    21601 ccacggcaga taaacaaccc gtttgctggt cagtgaccca acgctggcaa actgtcgcgc
    21661 tggggatgcc acgactctgt tcagcgcagc gtttggtggg ggcaatggtc gaggaaatct
    21721 tctctgtaaa tttggcgtaa ataatcagtt acatcaatga gtcctaaacg aaatccatgt
    21781 gtgaagttga tcacaaattt aaacactggt agggtaaaaa ggtcattaac tgcccaattc
    21841 aggcgtcaac tggtttgatt gtacattcct taaccggagg gtgtaagcaa acccgctacg
    21901 cttgttacag agattgcatc ctgcaattcc cgctcccctt ttgcggccgt cgcgctgatt
    21961 tttctggcgt ttgcggaaat gggccaactc tgcgaggaaa gctatgctga aaaggaaaaa
    22021 agtaaaaccg attacccttc gtgatgtcac cattattgat gacggtaaac tgcgtaaagc
    22081 cattaccgca gcatcactgg gtaatgcaat ggaatggttt gattttggtg tttatggttt
    22141 tgttgcttac gcattaggta aagttttttt cccgggggct gaccccagcg tgcagatggt
    22201 tgctgcactt gccactttct ccgttccctt tctgattcga ccgcttggcg gactcttctt
    22261 tggtatgttg ggcgataaat atggtcgcca gaagatcctc gctatcacta ttgtgattat
    22321 gtcgatcagt acgttctgta ttggcttaat accgtcctac gacacgattg gtatttgggc
    22381 accgattctg ctgttgatct gtaagatggc acaaggtttc tcggtcggcg gtgaatatac
    22441 tggggcgtcg atatttgttg cggaatactc ccctgaccgt aaacgtggct ttatgggcag
    22501 ctggctggac ttcggttcta ttgccgggtt tgtgctgggt gcgggcgtgg tggtgttaat
    22561 ttcgaccatt gtcggcgaag cgaacttcct cgactggggc tggcgtattc cgttctttat
    22621 tgctctgccg ttagggatta tcgggcttta cctgcgccat gcgctggaag aaactccggc
    22681 gttccagcag catgttgata aactggaaca gggcgaccgc gaaggtttgc aggatggccc
    22741 gaaagtctcg tttaaagaga ttgccactaa atactggcgc agcctgttga catgtattgg
    22801 tctggtaatt gccaccaacg tgacttacta catgttgctg acctatatgc cgagttattt
    22861 gtcgcataac ctgcattact ccgaagacca cggggtgctg attattatcg ccattatgat
    22921 cggtatgctg tttgtccagc cggtgatggg cttgctaagt gaccgttttg gccgtcgtcc
    22981 gtttgtgcta cttggtagtg ttgcactgtt tgtgttggcg atcccggcgt ttattctgat
    23041 taacagtaac gtcatcggcc tgatttttgc cgggttactg atgctggcgg tgatccttaa
    23101 ctgctttatg ggcgttatgg cttctacctt gccagcgatg ttcccgacgc atatccgtta
    23161 cagcgcgctg gcggcggcat ttaatatttc ggtgctggtt gccggtctga cgccaacact
    23221 ggcggcctgg ctggtcgaaa gctcgcagaa tctgatgatg cctgcctatt acctgatggt
    23281 agtggcggtg attggtttaa tcaccggcgt aaccatgaaa gagacggcaa atcgtccgtt
    23341 gaaaggtgcg acaccggcgg cgtcagatat acaggaagcg aaggaaattc tcgtcgagca
    23401 ttacgataat atcgagcaga aaatcgataa tattgaccac gagattgccg atttgcaggc
    23461 gaaacgtacc cgcctggtgc agcaacatcc gcgaattgat gaataagctg aaacggatgg
    23521 cctgatgtga cgctgtctta tcaggccaat tgaactctta aggttcactt aatctctgac
    23581 gcgcatactc tcctccaggt taacggagga gagtgcaatg aaaaaccgtg tttatgaaag
    23641 tttaactacc gtgttcagcg tgctggtggt cagcagcttt ctttatatct ggtttgccac
    23701 gttctgatct ttcttcagcc gtacccaggc ccgcgtgccg gaagtctctt gccggttttg
    23761 caggaaaaac tgcccgtgat gcaactgtgt aatgcggctg acaatactta accccagacc
    23821 aatcccgcca taacggctgt ccatacgtac aaacgcttta ctcaactccc cgcatttact
    23881 ctcatcaata cctggtcctt catcttcaac tgccatgacc gctccgccat cttcttgcag
    23941 cttaatcata atgttgctgc cttgcgggct gtaacgatgg gcgttttcta ccaagtttcg
    24001 caataacatc cgcagcaggg ttgcatcacc ctgaacggtg atgtcggcgg cgctctctgg
    24061 caatagcagg gtttgctgtc gctggtcgag catggtactg agttcgtcat acgaggggag
    24121 aatgacatct tccagcagtt ttacatgttg ataattaccg gaagaaaatg actgtccggc
    24181 acgcgccagt tgcagcagct gggagacgct ctccatcatc tgatcaagcc gtgccactaa
    24241 cggtgctaca tcaatgtgat gcgttttcgc cagcagttcc agatgcaaac gcacccccgc
    24301 cagtggcgtt cgcagttcgt gcgcgacgtc agcggtaaac aacctttcgt tatccagcgt
    24361 gctggtcagg cgactgacca gatcgttcaa cgccgaaacc accgcttcga tttcgagggt
    24421 ggcgctgtga atggcaatgg gcgttaagtt gtcggcggtg cgcgcttcca gctctttttg
    24481 cagctccgcc agcgggcggg tgatgcggcg taccgcctga tagcagataa atagcgtcag
    24541 gctgaccata aagacgccgg ggacaatcag gctggcgacc gcctcgcgga tctcacgcat
    24601 gatgtggcga tcgttgttgc gattgtcgcg tagcgcctgc tcaaacagct gaatctgctc
    24661 ggtactttca tgccatagcc agaagacgct gatcagctca aacaccaaca aaatggcccc
    24721 gatggtcaat atcagccgtt ggcgcagcga tattggtcgg cgcagaaaac gcatcagatt
    24781 caattagttt tcctcattcg cgaccagcat atagccaaag ccgcgcatgg tgcggatacg
    24841 ggctttgccc actttgtcgc gcagattgtg gatatgcact tccagggtgt tggtcgaggg
    24901 ttcattgtcc cagttataga tgtcgttgta gagaatttcc cgatgtaccg gactgcctgc
    24961 tttgagcatt aaccgtgaca gcagagcata ttctttgggc gtcagaatca actcttcacc
    25021 gcccatccat acctgacggc gacccatatt cagcgtcaga ttgccaacaa tcagctcact
    25081 ttcgccctga ttattatggc gtcgtagcag ggcgcggata cgggcgtgta actcttccag
    25141 cgcaaaaggc ttcaccagat agtcatcggc accgacatcc agcccggcga ttttgtcggt
    25201 cagcgtatcg cgagcggtga ggatcagtac cggcagggtg tattttttct gccggatacg
    25261 ggcgagaaaa tgcagtccat cttcgtcggg taaccctaaa tccagtacca ccaggctgta
    25321 atgaccgtcc tcaaggcttt gttccgccat ccgcgcggtt gtcacgccat cgcacgcgta
    25381 gccttcggtt tgcgccgcca gaatcagtcc ctgcaataac agcgtatcgt cttcaacaat
    25441 cagaattttc atttactcac tctcctgcaa gtttgcagaa tatcatccgc ggcctggtaa
    25501 tacttcgtct caacgccagt taatcccaat agcgtggaga ataaattgtc ttgtgaatag
    25561 tgttgcgttt gcgcctgttt ttgcaggcag ttctggtcaa cctgataccg cttttgataa
    25621 tcctccgaca gccacagcag cattggcacc tgtttttggc tatccggggc gatggcataa
    25681 ggcagaccgt gcagatagat gccattttca cctaacgatt caccgtggtc agaaagataa
    25741 accaggctgg tggtaaattt atcctgatgt tctttaagca gattaatcgc tttatcaaca
    25801 atatagtcga cgtaaaccag cgtgttgtcg taagtgttca ccagttgctc tttggtacag
    25861 gtctggatct cattggtgtc gcaggttggg gtaaatttcc tgaactgcgg cggatagcgg
    25921 ttgtaatagg tcggaccgtg gctgccgatg gtgtgtaaga caatcacgcc atcaccctgc
    25981 aggttattga tgtactcttc cagcccgtgg aacagcactt cgtcatagca ttcgccgttg
    26041 atgcactgat caggcagatt cagcgcggtg acgttctggt gaggcacgcg gtcgcaggca
    26101 cctttacagc cgccatcgtt gtcattccac agcacgttga tgcccgctcg ctgaatgata
    26161 tccagcacgc cttcctggtg ctgtgccagc tcttctttgt agtgctcacg cggcatatcc
    26221 gagaacatgc acggtactga aactgccgtt gccgtgccgc aagatgcggt attagggaaa
    26281 tagaccacgt tatctttcgc cagccgcggg ttagtttcac gcgggtagcc gttgagggag
    26341 aagttctccg cccgcgaggt ttcgccgaca atcaggatgg tcaaattttt acgtttttcg
    26401 ttctgcatta acgggttgcg gtgcgcgtct tcaccaattc gcaccagcgg cagatttgcc
    26461 agtcgctgat gggagtacca tgaccagctg gcaacaatgc tgttagaggg gcttaaggat
    26521 ttcaccagct ctttgttatt gcggaacaac gaggcgtagt ctttataaaa cagtgcggcg
    26581 accagcaaaa tcagcagtac agaaaccaga atattggctc cacggaaaag aacactgcgc
    26641 agacgcgagg tggcaggttt gatttttagc cagcaggcaa tcagcgcagc aagcacgccg
    26701 ctgaatccca gcgttaataa catttgcggt gtcatcagcg cataactttc tgctggagtg
    26761 gtatcaataa tattggcaat catcgagcgg tcgatgacga tgccgtaagt cattatgaaa
    26821 tattgtgcag ccgcgccaac cagaataaac aggcaggcca gtggtcgatt aagccataag
    26881 aaagagctta gtgtcaggac aatattaatc acgctgaaag cgacgaccgg catcgacaag
    26941 aaaaccagta cgttatgcag cgaatccagc ggcagcgcct gcaacacctg tttaaaaaag
    27001 gcaatattca ggcggataga gatataaaaa gcggccaaca atagccaggc gagtaaattc
    27061 aaagagggtc tttttagtag gcgcttcaac atcacggtgt ttccatcgaa caaagtgcgc
    27121 atatgctcgc aaagcaaaat taagccaacc ttaagttctt aaggttggct tttatgtttg
    27181 ctggattaag gggaattaat ctttgcttat tggtgcatct aagggatacg ggtttttatg
    27241 tagccggttg taattcagcg catacatggc ggtgatgacc atcagggtga caaatgacca
    27301 cataacctct ttcgccccgg accccaccac ggcccaaatg cagtagagga aggcaatggt
    27361 agtaacagcc agatatgccg ggcgtgcttt accaaagtga ccgtggccga gcagcagtaa
    27421 cgccgcacag gtgtaaagat atggcaccag tgtaaagatg accgagacgg aagaaaccag
    27481 accgaactct ttggtcgcgt ttggtgaaat gctgctgagc tggaagatgg tcatcaaaat
    27541 accgacgata atcagccccg ccactggcgt acccgcttta tttacacggg caaaaatc
//
LOCUS       DADLSB010000037        26913 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.037, whole genome shotgun sequence.
ACCESSION   DADLSB010000037 DADLSB010000000
VERSION     DADLSB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.037"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            126..449
                     /gene="iraM"
                     /locus_tag="JN331_001596"
     CDS             126..449
                     /gene="iraM"
                     /locus_tag="JN331_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7259769.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIQIC
                     PYGLK"
     gene            complement(552..1802)
                     /gene="icd"
                     /locus_tag="JN331_001597"
     CDS             complement(552..1802)
                     /gene="icd"
                     /locus_tag="JN331_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY7259770.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1974..2627
                     /gene="rluE"
                     /locus_tag="JN331_001598"
     CDS             1974..2627
                     /gene="rluE"
                     /locus_tag="JN331_001598"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY7259771.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTKRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGTELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2637..3098
                     /gene="nudJ"
                     /locus_tag="JN331_001599"
     CDS             2637..3098
                     /gene="nudJ"
                     /locus_tag="JN331_001599"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY7259772.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3152..4258
                     /gene="mnmA"
                     /locus_tag="JN331_001600"
     CDS             3152..4258
                     /gene="mnmA"
                     /locus_tag="JN331_001600"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY7259773.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4294..4935
                     /gene="hflD"
                     /locus_tag="JN331_001601"
     CDS             4294..4935
                     /gene="hflD"
                     /locus_tag="JN331_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY7259774.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4939..6309
                     /gene="purB"
                     /locus_tag="JN331_001602"
     CDS             4939..6309
                     /gene="purB"
                     /locus_tag="JN331_001602"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY7259775.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKNIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6478..7149
                     /gene="phoP"
                     /locus_tag="JN331_001603"
     CDS             6478..7149
                     /gene="phoP"
                     /locus_tag="JN331_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY7259776.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7149..8609
                     /gene="phoQ"
                     /locus_tag="JN331_001604"
     CDS             7149..8609
                     /gene="phoQ"
                     /locus_tag="JN331_001604"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY7259777.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAEESMLGGARMEVIFGRQHSAPKDE"
     gene            8685..9806
                     /locus_tag="JN331_001605"
     CDS             8685..9806
                     /locus_tag="JN331_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY7259778.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9855..11081)
                     /gene="pepT"
                     /locus_tag="JN331_001606"
     CDS             complement(9855..11081)
                     /gene="pepT"
                     /locus_tag="JN331_001606"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY7259779.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEALSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11331..12467
                     /gene="potA"
                     /locus_tag="JN331_001607"
     CDS             11331..12467
                     /gene="potA"
                     /locus_tag="JN331_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY7259780.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12451..13308
                     /gene="potB"
                     /locus_tag="JN331_001608"
     CDS             12451..13308
                     /gene="potB"
                     /locus_tag="JN331_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY7259781.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13305..14099
                     /gene="potC"
                     /locus_tag="JN331_001609"
     CDS             13305..14099
                     /gene="potC"
                     /locus_tag="JN331_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY7259782.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            14096..15142
                     /gene="potD"
                     /locus_tag="JN331_001610"
     CDS             14096..15142
                     /gene="potD"
                     /locus_tag="JN331_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY7259783.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(15298..16119)
                     /gene="cobB"
                     /locus_tag="JN331_001611"
     CDS             complement(15298..16119)
                     /gene="cobB"
                     /locus_tag="JN331_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY7259784.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16135..17046)
                     /gene="nagK"
                     /locus_tag="JN331_001612"
     CDS             complement(16135..17046)
                     /gene="nagK"
                     /locus_tag="JN331_001612"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY7259785.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17075..18319)
                     /gene="lolE"
                     /locus_tag="JN331_001613"
     CDS             complement(17075..18319)
                     /gene="lolE"
                     /locus_tag="JN331_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY7259786.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18319..19020)
                     /gene="lolD"
                     /locus_tag="JN331_001614"
     CDS             complement(18319..19020)
                     /gene="lolD"
                     /locus_tag="JN331_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY7259787.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19013..20212)
                     /gene="lolC"
                     /locus_tag="JN331_001615"
     CDS             complement(19013..20212)
                     /gene="lolC"
                     /locus_tag="JN331_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY7259788.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPVQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20474..21547
                     /locus_tag="JN331_001616"
     CDS             20474..21547
                     /locus_tag="JN331_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7259789.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21675..25121
                     /gene="mfd"
                     /locus_tag="JN331_001617"
     CDS             21675..25121
                     /gene="mfd"
                     /locus_tag="JN331_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY7259790.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25264..26226
                     /gene="ldtC"
                     /locus_tag="JN331_001618"
     CDS             25264..26226
                     /gene="ldtC"
                     /locus_tag="JN331_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAY7259791.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPIGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(26309..26566)
                     /gene="bhsA"
                     /locus_tag="JN331_001619"
     CDS             complement(26309..26566)
                     /gene="bhsA"
                     /locus_tag="JN331_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY7259792.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            26808..>26913
                     /locus_tag="JN331_001620"
     CDS             26808..>26913
                     /locus_tag="JN331_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7259793.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQH"
BASE COUNT         6621 a         6946 c         6793 g         6553 t
ORIGIN      
        1 tttgttttat ataagcaatc gttttaatta atttattttt tgaaggggta atatactcat
       61 atgcaaaata aagaaatgaa catccaaatg aactatatta aataccgtgg gataagacat
      121 aacaaatgaa gtgggtagta attgacacgg taattcaacc tacatgcggc atatcttttt
      181 cagccatatg gggtgatatg aaaatgatca tctggtatca atctactata tttcttcctc
      241 ctggaaggat atttacaccg gttaagcttg gtattatcct taaggaaaaa gaatatccta
      301 ttactattta taacattgca ccattcaaca agaatttatg gagtttactc aaaagcagtc
      361 aagagtgtcc tccaggagaa agcgaaataa caaataaatg tttgcataag agttgtatta
      421 tacaaatatg cccatatggg ctcaagtaat aggttttata taaatatgct cgatatgcta
      481 ttaataatat ccacataaat aaaacaacgg gcgtgttata cgcccgttta aatatttaac
      541 acatgtagtg attacatatt cttgatgatc gcatcaccaa actctgaaca tttcagcagt
      601 ttagcgcctt ccatcagacg ctcgaagtca taggttacgg tcttggcgtt gatcgcgcct
      661 tccatacctt taacaatcag gtctgcggct tcagtccagc ccatatggcg taacatcatc
      721 tctgcggaga gaataataga gccagggttt actttgtcct gaccggcgta tttcggcgca
      781 gtaccgtggg tggcttcaaa cagggcgcat tcgtcaccga tgtttgctcc aggggcgata
      841 ccgataccgc caacctgcgc tgccagagcg tcagaaatgt agtcaccgtt caggttcata
      901 caggcgataa catcatattc agccggacgc agcaggatct gttgcaggaa tgcatcagca
      961 atcacgtctt taatgacgat ctctttgccg gtattcgggt ttttaacttt cagccacggg
     1021 ccgccgtcga tcagttcacc gccaaattct tcacgcgcca gctggtagcc ccagtcttta
     1081 aacgcgcctt cggtgaactt catgatgttg cctttgtgca ccagggtcac agagtcacga
     1141 tcgttagcaa ttgcgtattc gatcgctgca cgaaccagac gtttggtgcc ttcttcagaa
     1201 cacggcttaa taccgatacc gcaatgttcc gggaagcgaa ttttcttcac gcccatctct
     1261 tcacgcagga atttaatcac tttctcggcg tcggcagagt cagctttcca ttcgataccc
     1321 gcataaatgt cttccgagtt ttcacggaag ataaccatat cggtcagttc agggtgttta
     1381 accgggcttg gagtgccctg atagtaacgt accggacgca ggcagatgta gagatccagt
     1441 tcctgacgca gggcaacgtt gagagaacga ataccgccac caaccggagt ggtcagcggg
     1501 cctttaatgg caacgcgata ttcacgaatc agatcaaggg tttcagcagg cagccagaca
     1561 tcctgaccat aaacctgtgt ggatttttca ccggtgtaaa tttccatcca ggagatttta
     1621 cgctcgcctt tataggcttt ctcgactgca gcgtcgacca ctttcagcat ggctggggtt
     1681 acatctacac cgattccatc accttcaatg taagggataa tcggattttc aggaacgttg
     1741 agtttgccgt tttgcagggt gatcttcttg ccttgtgccg gaacaactac tttactttcc
     1801 attcacctct ccttcgagcg ctactggttt gctcgtctgc caccacgttg catatgcgtt
     1861 tgcgtcctgc gatacggatg ctttagagca attttttgtt aatgatttgt aattggcttg
     1921 tcaatactac cctattgttt gccgtcatga aagatgcgcg ttattaggct ataatgcggc
     1981 aattcataat ctctgaaaat accatgcaaa aaacttcttt tagaaatcac caggttaagc
     2041 gattcagctc gcaacgttct accaagcgta aacatgaaaa ccagcccacg cgtgtgatcc
     2101 tcttcaataa accctacgat gttcttccgc agttcaccga tgaagccgga cgcaaaacat
     2161 taaaagaatt catcccggtt cagggcgttt atgcagcagg tcgccttgac cgcgatagcg
     2221 aagggttgct ggtgctgacc aataacggcg cgttgcaggc gcgtttaacc cagccgggta
     2281 aacgcaccgg aaaaatctat tatgtgcagg tggaaggtat tcccacacaa gacgcacttg
     2341 aagccttgcg caatggcgta accttaaatg atggccctac cctgcccgca ggcacggagc
     2401 tggttgacga acccgcgtgg ttatggccgc ggaatccacc aattcgtgaa cgcaaaagta
     2461 ttcccaccag ctggctgaag atcaccttat atgaaggacg taatcgccag gtgcgccgca
     2521 tgaccgccca tgttggtttc cccacgctgc gactgattcg ctatgcgatg ggagattact
     2581 ctttggataa tcttgccaat ggcgaatggc gagaagtgac agattaagga tgcaaaatgt
     2641 ttaaaccgca cgttaccgtt gcttgcgtgg tgcacgcaga aggcaaattt ttagtcgttg
     2701 aagagacgat taatggtaaa gcgttatgga accaacctgc cgggcatctg gaagccgatg
     2761 aaaccttagt ggaagccgcc gcccgtgaac tgtgggaaga aaccggcatc agcgcgcagc
     2821 cgcaacactt tattcgtatg catcagtgga ttgcgccaga taaaacgccg tttttgcgtt
     2881 tcctctttgc cattgagctt gagcaaatat gcccgaccca gcctcatgac agcgatatcg
     2941 actgctgccg ttgggtcagc gccgaagaaa ttttacaggc gtcaaatctt cgttcgccgc
     3001 tggtggcgga aagtattcgt tgttatcaaa gcgggcaacg ttatccgctg gagatgattg
     3061 gcgattttaa ctggcctttt acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc
     3121 cgccttgaag ttcaatgtcg tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt
     3181 cggcatgtcc ggcggtgtcg attcctccgt ttctgcctgg ctgttgcaac aacagggata
     3241 tcaggtcgaa ggcctgttta tgaagaactg ggaagaagac gacggtgagg aatattgcac
     3301 agcggcagcg gatctggctg atgcccaggc ggtctgcgac aagctcggca ttgaactgca
     3361 caccgttaac tttgctgccg agtactggga caacgtcttc gaactgttcc ttgccgaata
     3421 taaagccggt cgcacgccga atccggatat tctgtgcaac aaggagatca aatttaaagc
     3481 cttcctcgaa tttgccgccg aagatttagg tgccgattat atcgctaccg gtcattacgt
     3541 acgtcgggcc gatgtcgatg gcaagagccg cctgctgcgt ggtctggaca gcaataaaga
     3601 ccagagctac ttcctttata cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt
     3661 cggcgaactg gaaaaaccgc aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc
     3721 gaagaaaaaa gactctaccg gcatttgctt cattggcgaa cgtaaattcc gcgagttctt
     3781 gggccgttat ctcccggcgc aaccgggcaa aatcattacc gtcgatggcg atgaaattgg
     3841 tgagcaccag gggctgatgt atcacactct cggtcagcgt aaaggtctgg gtatcggtgg
     3901 caccaaagaa ggcaccgaag aaccgtggta tgtggtggac aaagacgtcg aaaacaacat
     3961 tctggttgtc gctcagggcc atgaacaccc gcggctgatg tctgtcgggt tgattgccca
     4021 gcagctgcac tgggtcgatc gcgaaccatt caccggcact atgcgttgca cggtaaaaac
     4081 ccgctatcgc cagaccgaca ttccgtgcac cgtcaaggcg ctggacgacg atcgcattaa
     4141 agtgattttc gatgagccgg ttgccgccgt gacgccgggc cagtctgccg tcttctataa
     4201 cggtgaagtg tgcctcggtg gcggtattat tgagcagcgt ctgccgctgc cggtctgatt
     4261 attatcttta cttaaaacaa ggaagcagtg aacgtggcaa agaattacta tgacatcacc
     4321 ctcgccctgg ccggtatttg tcagtcggca cgcctggtgc aacaactcgc tcaccagggg
     4381 cattgtgatg ccgatgcgct acacgtctca ctcaacagta ttattgatat gaaccccagc
     4441 tcgacgctgg cggtttttgg cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg
     4501 ctcggcgtgc tcaatgccag cagttgccag ggcttaaacg ccgaattaac ccgctacaca
     4561 ctcagcttga tggtgcttga gcgcaaactc tcctcagcga aaggcgcgct cgacactctg
     4621 ggcaaccgga tcaacggcct gcaacgccag ctcgaacact tcgatttaca gtccgaaacg
     4681 ctgatgagcg cgatggctgc tatctatgtt gatgtgatca gcccgcttgg cccgcgcatt
     4741 caggtcaccg gttcccctgc tgtactgcaa agcccacaag tgcaggcgaa agttcgcgca
     4801 accctgctgg caggcattcg cgccgccgtg ctctggcacc aggtcggcgg cggacgtcta
     4861 caactgatgt tttctcgtaa tcgcctgacc actcaggcaa aacaaattct tgctcattta
     4921 accccggagt tgtgatctat ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga
     4981 cgctacggcg ataaagtcag cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa
     5041 ttccgtgtac aagttgaagt acgttggctg caaaaactgg ccgcgcacgc agcgatcaag
     5101 gaagttcctg cttttgctgc cgacgcaatc ggttaccttg atgcaattgt cgccaatttc
     5161 agcgaagaag atgccgcacg catcaaaaac atcgagcgta ccactaacca cgacgttaaa
     5221 gcggttgagt atttcctgaa agaaaaagtg gcggagatcc cggaactgca cgcggtttct
     5281 gaattcatcc actttgcctg tacttcggaa gatatcaata acctctccca cgcattaatg
     5341 ctgaaaaccg cgcgtgatga agtgatcctg ccgtactggc gtcaactgat tgatggcatt
     5401 aaagatctcg ccgttcagta ccgcgatatc ccgctgctgt ctcgtaccca cggtcagcca
     5461 gccacgccgt caaccatcgg taaagagatg gcaaacgtcg cctaccgtat ggaacgccag
     5521 caccgccagc ttaaccaggt ggagatcctc ggcaaaatca acggcgcggt cggtaactat
     5581 aacgcccaca tcgccgctta cccggaagtt gactggcatc agttcagcga agagttcgtc
     5641 acctcgctgg gtattcaatg gaacccgtac actacccaga ttgaaccgca cgactacatt
     5701 gccgaactgt ttgattgcgt tgcgcgcttc aacaccattc tgatcgactt tgaccgtgac
     5761 gtctggggtt atatcgccct taaccacttc aaacagaaaa ccattgctgg tgagattggt
     5821 tcttccacca tgccgcataa agttaacccg atcgacttcg aaaactccga agggaatctg
     5881 ggcctttcca acgcggtatt gcagcatctg gcaagcaaac tgccggtttc ccgctggcag
     5941 cgtgacctga ccgactccac cgtgctgcgt aacctcggcg tgggtatcgg ttatgcgctg
     6001 attgcgtatc aatccaccct gaaaggcgtg agcaaactgg aagtgaaccg tgaccatctg
     6061 ctggatgagc tggatcacaa ctgggaagtg ctggcagaac caatccagac agttatgcgt
     6121 cgctatggca tcgaaaaacc gtacgagaag ctgaaagagc ttactcgcgg taagcgcgtt
     6181 gacgccgaag gcatgaagca gtttatcgat ggtctggcgt tgccagaaga agagaaagcc
     6241 cgcctgaaag cgatgacgcc ggctaactat attggtcgag ctatcacgat ggttgatgag
     6301 ctgaaataag cctcaaatca gtgccggatg gcgatgctgt ccggcctgct tattaagatt
     6361 atccactttt tcttttttca ctttacctcc cctccccgct ggtttattta atgtttaccc
     6421 ccataaacac ataatcgcgt tacactattt taataattaa gacagggaga aataaaaatg
     6481 cgcgtactgg ttgttgaaga caatgcgttg ttacgtcacc accttaaagt tcagattcag
     6541 gatgctggtc atcaggtcga tgacgcagaa gatgccaaag aagccgatta ttatctcaat
     6601 gaacatttac cggatattgc gattgtcgat ctcggattgc ctgacgagga cggtctgtca
     6661 ctgattcgcc gctggcgtag caacgatgtt tcactgccga ttctggtatt aaccgcccgt
     6721 gaaagctggc aggacaaagt cgaagtatta agtgccggtg ctgatgatta cgtgactaaa
     6781 ccgtttcata ttgaagaggt gatggcgcga atgcaggcat taatgcggcg taatagcggt
     6841 ctggcttcac aggtcatttc gctccccccg tttcaggttg atctctctcg ccgtgaatta
     6901 tctattaatg acgaagtgat caaactgacc gcgttcgaat acaccattat ggaaacgttg
     6961 atacgcaata atggcaaagt ggtcagcaaa gattcgttaa tgctacaact ctatccggat
     7021 gcggagctgc gggaaagcca taccattgat gtactgatgg gacgtctgcg caaaaaaatt
     7081 caggcacaat atccgcaaga agtcattacc accgttcgcg gccagggcta cctgttcgaa
     7141 ttgcgctgat gaaaaaatta ctgcgtcttt ttttcccgct ctcgctgcgg gtacgttttc
     7201 tgttggcaac ggcagcggta gtactggtgc tttcgcttgc ctacggaatg gtcgcgctga
     7261 tcggttatag cgtcagtttc gataaaacta cgtttcggct gttacgtggc gagagcaatc
     7321 tgttctatac ccttgcgaag tgggaaaaca ataagttgca tgtcgagtta cccgaaaata
     7381 tcgacaagca aagccccacc atgacgctaa tttatgatga gaacgggcag cttttatggg
     7441 cgcaacgtga cgtgccctgg ctgatgaaaa tgatccagcc tgactggctg aaatcgaatg
     7501 gttttcatga aattgaagcg gatgttaacg ataccagcct cttgctgagt ggagatcatt
     7561 cgatacagca acagttgcag gaagtgcggg aagatgatga cgacgcggag atgacccact
     7621 cggtggcggt aaacgtctac ccggcaacat cgcggatgcc aaaattaacc attgtggtgg
     7681 tggataccat tccggtggag ctaaaaagtt cctatatggt ctggagctgg tttatctatg
     7741 tgctctcagc caatctgctg ttagtgatcc cgctgctgtg ggtcgccgcc tggtggagtt
     7801 tacgccccat cgaagccctg gcaaaagaag tccgcgaact ggaagaacat aaccgcgaat
     7861 tgctcaatcc agccacaacg cgagaactga ccagtctggt acgaaacctg aaccgattgt
     7921 taaaaagtga acgcgaacgt tacgacaaat accgtacgac gctcaccgac ctgacccata
     7981 gtctgaaaac gccactggcg gtgctgcaaa gtacgctgcg ttctctgcgt agtgaaaaga
     8041 tgagcgtcag tgatgctgag ccggtaatgc tggagcaaat cagccgcatt tcacagcaaa
     8101 ttggctacta cctgcatcgt gccagtatgc gcggcgggac attgctcagc cgcgagctgc
     8161 atccggtcgc cccactgctg gacaatctca cctcagcgct gaacaaagtg tatcaacgca
     8221 aaggggtcaa tatctctctc gatatttcgc cagagatcag ctttgtcggt gagcagaacg
     8281 attttgtcga ggtgatgggc aacgtgctgg ataatgcctg taaatattgc ctcgagtttg
     8341 tcgaaatttc tgcaaggcaa accgacgagc atctctatat tgtggtcgag gatgatggcc
     8401 ccggtattcc attaagcaag cgagaggtca ttttcgaccg tggtcaacgg gttgatactt
     8461 tacgccctgg gcaaggtgta gggctggcgg tagcccgcga aatcaccgag caatatgagg
     8521 gtaaaatcgt cgccgaagag agcatgctgg gcggtgcgcg gatggaggtg atttttggtc
     8581 gccagcattc tgcgccgaaa gatgaataaa tatgtccata cttcacgcat tacgttaagc
     8641 atccgttata atcggttgca gataccagcc tgtggatgct taacatggaa taccaactca
     8701 ctcttaactg gcccgatttt cttgaacgtc actggcagaa acgcccggtg gtgttaaaac
     8761 gcggctttaa taattttatt gacccgatct ctccagacga gttggcgggt ctggcgatgg
     8821 aaagcgaagt tgacagtcga ctggtcagtc accaggatgg caaatggcag gtcagccacg
     8881 gcccgttcga aagctacgat catctcggtg aaaccaactg gtcattactg gtacaggcag
     8941 taaaccactg gcatgagccg accgccgcgc tgatgcgacc gttccgtgaa ctaccggact
     9001 ggcgtattga tgatctgatg atttcttttt ctgtacctgg cggcggcgtc ggcccgcatc
     9061 tcgatcagta cgacgtgttt atcattcagg gtaccggacg tcgtcgctgg cgagtgggcg
     9121 aaaagctgca aatgaaacag cactgcccac atccggatct gttacaggtc gatccgttcg
     9181 aagccatcat cgatgaagag ctggagcctg gtgatattct ttatattccg ccaggattcc
     9241 cgcatgaagg ctacgcgctg gaaaatgcga tgaactattc cgtgggtttt cgcgcgccaa
     9301 atacgcggga attaattagc ggatttgccg attatgtgct gcaacgtgaa ctgggcggca
     9361 actactacag cgatccggat gttccacctc gcgctcatcc tgcggatgtt ctgccgcaag
     9421 agatggataa actgcgtgag atgatgctcg aattgatcaa ccagccggaa cactttaagc
     9481 aatggtttgg cgagtttata tcccagtcac gtcatgaact ggatatcgcg ccgccggaac
     9541 cgccttatca gccagatgaa atctacgatg cgctgaaaca aggtgatgtg ctggtgcgcc
     9601 tgggtggtct gcgcgtattg cgcattggcg acgacgtgta tgccaatggt gagaagatcg
     9661 attccccgca ccgtccggca ctggatgcac tcgccagcaa cattgcgctg actgcggaga
     9721 attttggcga tgcgctggaa gatccgtcat tcctcgcgat gctcgcggcg ctggtcaata
     9781 gcgggtattg gttcttcgaa gggtaagttt ggatttaagc cggatgcagc atcacatgcc
     9841 gcatcccttt tcgcttactt ccgttgcgcc gttaactcgg caatacggac gatcacctgc
     9901 accgcttttt ccataccttc cagagtcaca aactcatgct taccatgata gttgtaaccg
     9961 ccagtgaaca ggttcgggca aggtaatccc ataaacgaca actgcgcgcc gtcggtaccg
    10021 ccgcggatcg gtttcagttc cggttcaata tcgcagtcac gcatcgcctg ctgggcgata
    10081 tcgagaatat gcggatgctc aaccactttc tcgcgcatat tgtagtaact gtcttcaatc
    10141 accaattcaa tgtagcaatc aggatgtaac cctttgccca cttttttggc gatctccatc
    10201 attttacgtt tacgcgcttc aaactgttta cggtcgaaat cacggatgat gtagtgcata
    10261 tcggcccgtt caacggtgcc tttcatgctc gccaggtgat agaaaccttc atagccttct
    10321 gtcatttccg ggctttcatc cgccggaact tccgcatgaa tacgtgccgc cagcgacagc
    10381 gcatttacca tcactccttt cgccgtgccc ggatgaacat tgttaccgac aattttgata
    10441 ttgaccgatg cggcgttgaa gttttcaaac tccagttcgc ctacgccacc accgtcaaca
    10501 gtgtaagccc agcgggcatc gaaggcatca acatcaaaat gtttcgcccc tttgcccact
    10561 tcttcatccg gggtaaaggc gacgcgaata tcaccatgcg gaatgttttt ctgttgcaat
    10621 accgccagcg cggtcatgat ttctgcaata cctgctttgt catcggcacc taacaaggtt
    10681 ttaccatcgg tggtaatcaa cgtctgaccc agtagctgat gcagcaccgg gaacataacc
    10741 ggtgataaag cttcatcgcc gatacccagc gcaatatcgc caccgcgata gttttcaaca
    10801 atttgcggat taacattttt gccgctgcaa tccggtgagg tatccacatg agaaataaag
    10861 ccaatcgccg ggatatcgcc agggacgtta gccggtaacg tcgccatcaa agtgcccttc
    10921 tcacttaagg tcacattgat aagccccatt tcttcgagct gctctttcag cagatgcaat
    10981 aacttccatt ggccttccgt gctgggaacc tgtctcaccc ctgcttttga ttgggtatcc
    11041 agagacacgt agttcaaaaa tcgctcaagt agtttatcca tgtagtcacc ctcacttttt
    11101 gtgacaacat tattaagaag caagaaaaga caaatattgc gtcaggtcac ttttacccct
    11161 gcaagcggga atatttatca gcattacctt aatgaatata aagctaagcc agtaattcac
    11221 aacaaagatt ggcgattcaa gcggtttgcc gtagaattac cgccctcatt aagagtcacc
    11281 aaggtggtta accacaaacc ccgcatcggt aagccatccg ttgcgtttac atgggacaga
    11341 gtaaaaaatt gaataaacaa ccgagttcgc tttcaccgct ggtgcaattg gcgggaattc
    11401 gcaaatgctt tgatggtaaa gaggtcattc cccagctgga tctgactatc aacaatggcg
    11461 agttcctcac gctgcttggc ccttctggct gcggtaaaac aaccgttctt cgcctgattg
    11521 caggtctgga aactgttgat tccggacgca tcatgctgga taacgaggac atcacccacg
    11581 ttccggcgga aaaccgctat gtgaacactg ttttccaaag ctatgcactt ttcccccaca
    11641 tgaccgtgtt cgaaaatgtg gcctttgggt tgcgcatgca aaaaaccccc gctgctgaaa
    11701 ttacgccccg cgtgatggaa gccctgcgga tggtgcagct ggaaaccttc gctcaacgta
    11761 aaccgcatca gctctctggt ggtcaacagc aacgcgtcgc cattgctcgc gcggtggtta
    11821 acaagcctcg tctgttgttg ctggatgagt cgctctcagc gctggattac aaactgcgta
    11881 agcaaatgca gaacgagctg aaagcgttac agcgtaagct tggcattaca ttcgttttcg
    11941 tgactcacga ccaggaagaa gcactcacca tgtcggacag gattgtggtg atgcgcgatg
    12001 gtcgcattga gcaagacggc acgccgcgtg aaatctacga agagccgaaa aacctgtttg
    12061 ttgccgggtt cattggcgaa attaatatgt ttaacgccac ggtgatcgag cgactcgacg
    12121 agcagcgcgt acgcgccaac gttgaaggcc gcgaatgtaa tatctacgtt aacttcgccg
    12181 ttgaaccggg gcaaaaactg catgttctgc tgcgcccgga agacttacgt gttgaagaga
    12241 ttaacgacga caaccacgct gaagggctga ttggttacgt tcgtgagcgt aactacaaag
    12301 gcatgacgct ggagtcggtt gttgaactgg aaaatggcaa gatggtgatg gtcagcgaat
    12361 tcttcaatga agacgatcct gactttgacc actctctcga ccaaaaaatg gccattaatt
    12421 gggtagaaag ctgggaggtc gtactggctg atgaagaaca caagtaagtt ccagaatgta
    12481 gtgattgtca ctattgtcgg ttggcttgtg ttgtttgtct ttctgcccaa cctgatgatc
    12541 attggcacca gctttttgac ccgcgacgac gccagtttcg tcaaaatggt ctttacgctg
    12601 gataactaca cgcgtctgct cgatccgctc tattttgaag tgctattgca ctcgctgaat
    12661 atggcgctga tcgccaccct cgcctgcctg gtgctgggct atccatttgc ctggtttctg
    12721 gcgaagctgc cacacaaggt gcgtccgctg ctgctgtttc tgctgattgt tccgttctgg
    12781 accaactcat tgattcgtat ctacgggctg aaaattttcc tcagcaccaa aggctatctc
    12841 aacgagtttt tgctctggct gggcgttatc gacacaccaa tccgtatcat gttcacgccc
    12901 agtgcggtga ttatcggtct ggtttacatt ctgctgccgt ttatggtgat gccgctgtac
    12961 tccagtatcg aaaaactgga taagccgttg cttgaggcgg cgcgcgatct cggtgccagc
    13021 aagttacaga cttttatccg catcattatt ccactgacga tgccggggat tattgccgga
    13081 tgtctgctgg tgatgctgcc agcgatgggc ctgttctatg tgtccgacct gatgggcggt
    13141 gcgaaaaacc tgctgatcgg taacgtcatc aaggtacagt tcctcaatat tcgtgactgg
    13201 ccgtttggtg cagccaccag cattacgctg actatcgtaa tgggcctgat gttgctggtt
    13261 tactggcgcg cttctcgttt gctgaataag aaggtggaac tcgaatgatc ggtcgactgc
    13321 ttcgcggcgg ttttatgacc gctatctacg cgtacctgta tatcccaatc attattttga
    13381 ttgtgaactc ctttaacagc tcgcgctttg gcatcaactg gcagggtttt accaccaaat
    13441 ggtatagcct gctgatgaac aacgacagcc tgttacaggc agcgcagcat tcactaacaa
    13501 tggcggtgtt ttcggcgacg tttgctacgc ttatcggttc actgacggca gttgcgctgt
    13561 accgttatcg ctttcgcggt aagccgttcg ttagcggaat gctgtttgtg gtaatgatgt
    13621 caccagatat cgtgatggca atttcgctgc tggtgctgtt tatgctgctg ggtattcagc
    13681 ttggcttctg gtcgctgctg ttctcgcata tcaccttctg cctgccattt gtggtggtga
    13741 cggtgtattc gcgcctgaaa ggttttgacg tgcggatgct ggaagcggcg aaagatctcg
    13801 gtgccagcga atttaccatt ctgcgaaaaa tcattctgcc actggcaatg ccagcggtgg
    13861 cggcgggctg ggtgttaagc tttaccctgt cgatggacga tgtggtagtt tcttcgttcg
    13921 tcaccggacc aagttatgaa attctgccgt taaaaattta ttcgatggtc aaagtcggcg
    13981 tatcgccgga agttaacgcg ctggcaacca tattactggt gctgtcgctg gtgatggtta
    14041 ttgccagcca gcttattgct cgtgataaaa cgaaaggtaa cacaggggac gttaaatgaa
    14101 aaaatggtca cgccacctgc tcgcggcggg tgctctggca ctgggcatga gcgccgctca
    14161 cgccgatgac aacaacacgc tgtatttcta caactggacc gagtacgtgc cgccaggact
    14221 gcttgaacag ttcaccaaag aaaccggtat taaggttatc tattcgactt acgagtcgaa
    14281 cgaaaccatg tacgccaagc tgaaaacgta caaagacggt gcctatgacc tagtggttcc
    14341 ttcaacctat tacgtcgata aaatgcgtaa agaggggatg atccagaaga tcgacaagtc
    14401 gaagttaacg aacttcagca atctcgatcc agacatgctc aacaagcctt ttgacccgaa
    14461 taacgactac tccattccgt atatctgggg tgcgacggcg attggtgtta acggtgatgc
    14521 ggtggatccg aaatctgtca ccagctgggc cgatctgtgg aagccagagt acaaaggcag
    14581 cctgctgttg accgacgatg cccgtgaagt gttccagatg gcgctgcgta agctgggcta
    14641 ctccggtaac accaccgatc cgaaagagat tgaagctgca tataacgagc tgaaaaaact
    14701 gatgccaaac gtcgcagcgt ttaactccga taacccggct aacccgtaca tggaaggcga
    14761 agttaacctc ggcatgatct ggaacggttc tgctttcgtt gcacgccagg cgggtacgcc
    14821 tattgacgtg gtgtggccga aagaaggcgg catattctgg atggacagcc tggcgatccc
    14881 ggcaaatgcc aaaaacaaag aaggcgcgct gaaattgatc aacttcctgc tgcgcccgga
    14941 tgtggcaaaa caggttgctg aaactatcgg ttatccaacg ccaaaccttg cggcgcgtaa
    15001 gctgttaagt ccagaagtgg caaacgataa gacgctctac ccggatgctg aaaccattaa
    15061 aaatggcgaa tggcagaatg acgttggcgc agccagcagc atttatgaag agtattatca
    15121 gaagctgaaa gcaggacgtt aattcagcaa gcgggcggtg gtattaccgc ccgtttttta
    15181 gttagcaagg atgtggctaa tgagtcagga cagtaaagtt ttatcccgct ctttcgtcgg
    15241 tattgtggtc cgcttgtagg cctgataagc gtagcgcatc aggcaatgct tcctgcttta
    15301 taagcccttc agcaactttt caacaaattc tggcaccacc tggcttgccg ggccgtaata
    15361 tttctcggca aattcattac caacctgact cggttcaaga ttcagttcaa cggtgtgagc
    15421 accatgtagt ttggcttcgt gtacaaaccc agccgccgga tagacatgcc cggaagtacc
    15481 aatggcaata aagacatccg ccatcgacaa cgccatataa atttcatcca tgccgagtgg
    15541 catttcgcca aaccaaacga cgtgcgggcg caagggtgcc gggaactggc aacaatggca
    15601 tttatcttct ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt gcgaacaacg
    15661 aactttcagc aattccccat gcatatgaat gacattggta ttacctgcgc gttcatgcag
    15721 gttgtctata ttctgcgtta ccagcaaaaa gcgatcgccg agagcgtctt gcagtttagc
    15781 cagcgcaaga tgcgcggcgt taggctgaat ttctggctgc tgcagctgtc gacgacgggc
    15841 gttataaaac gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg ttgccacatc
    15901 ttcaacccga tgttcttccc acaggccatc tgcggcgcga aaggtacgaa tacctgattc
    15961 cgcagaaatt cctgcccctg tcagtacgag tactcttggt ttttccattg cttccggcac
    16021 cactttatct ctgaaaaaaa tacgctgacg caaacgctcg cgcaggcggc gtttattttt
    16081 acgaaaacga cttaaccgat gaccccgacg cgacagcata gcaacctctg ttgtttaatc
    16141 ggttagatgt aggaaggccg caccacgcat tcctcccgca tcaccgtggc gcgcgcgttc
    16201 aatgcgcgga acacgagcta caggtaagag atgacaaggc aacctgtccg ccagttgcgt
    16261 tgtgattgcc gggaaattcg ataagccacc accaatgacg accaggtcag ggtcaacaat
    16321 ggtcaggata tttcccagag aaaccgctaa taaatccaga taacgctcaa cgtgcgccct
    16381 tgcctgctca tcgccttgat cataaagcgc aataatttcg ggagcctgca acggttgatg
    16441 ataatagtgt tgatacagcc acgcaaaacc gcgaccagac agataatttt caatgcagcc
    16501 atgctgacca cagccgcagc ggcgtaacgg gaaatccaac cccatcatgg ttaacgcatc
    16561 aaccggcaga cgcatatggc caaactcgcc ggtaatgtag cttttgccag taatcggttt
    16621 gccgttgaaa atcagcccgc cgccaacgcc ggtgccgaga attaacccca tcaccagtgg
    16681 atattgcgta aattcatcat cccaggcttc tgaaagggca aaacagttgg catcgttatc
    16741 aaggcgtaca tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt tgccgctggc
    16801 agcggggaca ttagcggcat acagcttacc atcttctgtt tccggcattc ccggaatacc
    16861 gatgccgaca gaacctttac agccaaaacg ttggtcagct tcagctacca gctcacacac
    16921 tgcatctaaa aatgcgtcat agctgtcacg cggtgtcggc acccgctttt cccactgcaa
    16981 ctgccgaccg ctatcaaaca cgccaagcgc aatttttgtt ccaccaatat caaacccgta
    17041 atacattgcc gctccttgtt ttaatgtact gcctttactg gccgctaagg actcgcgcag
    17101 ggtcaatatt actggcgcgc cgcgccggat accaacttgc caaaagactc agcaacaatg
    17161 ctgtgaccag tacgtagaag acgtccagcc agtgcaattc cgatggcagg aagtcaataa
    17221 aatagatatc gctggagagg aactgatgac cgatcagctt ttcaatccac tcaataatcg
    17281 gggtaagttg cagtgaaaca actacgccga taaccacccc acacaggctg ccgaacagcc
    17341 ctgccagcaa tccataccag acaaagatgg cgcgaattaa accatctttc gcccccagcg
    17401 ttcttaatac tgcgatatcg ccactcttgt ctttcaccgc catcactaag gtggagacga
    17461 tgttgaaaca ggccacgcca atcaccagta ccatcgccag atacataatg gcgcggatca
    17521 tctggatatc gcgatacata tagccgtaag taccaatcca acttttaata taaacatagc
    17581 tgttggtcac ttcacccgca tcgcgtacca gcttattggc gttgaaaaca tccgtcattt
    17641 taagggcaat acctgacacg ctggaaccca tatcaagata ttgttgggca tccgccagcg
    17701 ggatcatggc aaaactgtga tcgagttgac cactcaactg caaaataccg gcaatgtgca
    17761 aacgcacacg ttttggctgc atcagtttat gctcgggatt cgagttgggg atcatgatcg
    17821 acacccaatc gccctgcttc actttcagcg catccgccac gcctttgccg atgataattt
    17881 gctgttcgcc cgctttaaaa ttgcgccacg catccccctg aacaaacgag ggtaatgcgc
    17941 tcagacgctg ttcctgttgt gggttaacgc ccttcacctg gattgcgcgc agattcgctc
    18001 cactttccac cagaccggtg aaattgatat acggcgcagc ggcggcaata cccggcactt
    18061 tctgcacgtt atccagtgct tcctgccagt tagtccatgg ctgatttacc gcttcgattt
    18121 cgccatgcgg caccaccgcc agaatgcggt tattcagttc gcgttcaaag ccgttcatcg
    18181 cgcttaagcc gacgatcaat accgccacgc cgagggcaat gccaatggta gaaatgacag
    18241 agattagcga caccatgccg ccgcgtcgcc gtccgcgact aaaacgcagg ccaatcaata
    18301 acgataaagg catcgccatt actccgcccc catcaggctc agttccgccg tcagacgtcc
    18361 atcacgcatt tccagttggc ggctcatacg tttcgccagt tgcaggtcgt gagtgaccac
    18421 caggaaagcg gtgccctgca agcgattcaa ctccccaagc aactggaaga tgctgtcggc
    18481 gttacgcgca tcgaggttac cggtaggttc atccgccagt accagacgcg gattattgac
    18541 cagcgcacgg gcaatcgcta cacgctggcg ttcgccgcca gaaagttcag atgggcggtg
    18601 attcgcacga tgctccagcc ccaccgcttt taacatctcc agtgcacggc tgttgatttc
    18661 agcgggcttt ttcttgccaa tcagcagtgg catagccacg ttttccaggg cagtaaaatc
    18721 cggcagcagg tggtgaaact gataaataaa gcccagcttc tggttgcgca gttctgcttt
    18781 cgccgctgaa gacagtttgc tcatcggctg accgttaaag atcacatcgc cggaggttgg
    18841 cgtatccagc ccgcctaaca ggtgcagcaa ggtactttta ccggaaccag agctaccgac
    18901 aattgccatc atttcacctt cgcccacgct gaaactgaca ttgtgcagca catcggtttg
    18961 cacactgcct tcctgatagc gtttgcacag gttgtcgcat tgcaacagga tcttattcat
    19021 aacgtaaagc ctcagcgggt tgtgtggcgg cagcgcgcca tgaagggtaa agcgtagaca
    19081 gcagcgcgat agccatcgcc accagcgcaa taacaatgac ctgtaaaggt tcgatagcca
    19141 ccggcagcgc cgcgccatca agcaggacgc cgattatcgg catcagatta tttaactggc
    19201 tggcaagcag tgcgccaagc gccgctccga ggattgcacc gataatcccg gcgctggccc
    19261 cttgcaccat aaagaccatc atgatttgtc gcggagttaa gccttgcgtt tgcaggatcg
    19321 cgacttcgcc ctgcttctcc atcaccatca gccccagcga ggtaataatg ttaaacgccg
    19381 caacagcgac aatcaggctc agcagtaagc ccatcatatt tttttccatg cgtacggcct
    19441 ggaacagctc gcctttacgg tcacgccagt cctgccattt gctgccttca ggcagttttt
    19501 gctgacttaa agagtcaact ttcagcggct catccagcca caaacgccag ccggtaatat
    19561 tggctgccgg ataacgcatc agacgcgagg catcctcaat attcaccagc atttcatagc
    19621 catcgacttc actgttggcg gcgaaagtgc caatcacatt gaacaggcgc tgacttggaa
    19681 tacgccccat cggcgtgaac tggctggcag atggcaccat cacgcggatt tgatcaccgc
    19741 gattgacgcc caactgtgag gcaagttgtt cgccgaggat gacattatat ttccccggct
    19801 cgaggtcagt ttgtttcaca ttgaccagat acggcgttag tggatctttt tgcaccgggt
    19861 cgataccgag catcaccccg accgccacgc tgcgcgcgct ttgcagtacc acatcaccgg
    19921 tagtaatagg tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc gggagttgct
    19981 gcgggttaag agagccatgc tcagaagaga gaattgcctg tggcatcagg ccaaggatgt
    20041 tgttttgcag ctcgcgctca aagccgttca tcactgacaa tactgtgacc agcgccatca
    20101 ccccgagggt aatgccgatg gtagaaagcc aggagacgaa acgaccgaag cgatccgctg
    20161 cacgcccacg catgtaacgc aggccaataa atagagcgac aggttggtac atgaaatccg
    20221 tctggttgct gtagcaaaga cacgagtata taagcgaaag caagatgggt aaacgtctga
    20281 actgtaagtg atcgtcaggg ctaatctgaa aagcccccgt aaatcaatcg gctaaattgc
    20341 catgttttat catcaatctt gccgcctgat cttaatctga aaaatcatcc aatacagaca
    20401 gatacccatt acacctcggg tcataaccag caggatcaac cgcatgtaaa ttaaaaaaga
    20461 gcgtgttgtc ctgatgaaac aaaaagagct atggattaac cagatcaaag ggttatgtat
    20521 ttgtctggtg gtgatttatc actcggtcat taccttttat ccgcatatga ccacttttca
    20581 gcatccgtta tcggaagtcc tgagcaaatg ctggatctat ttcaatcttt accttgcccc
    20641 ctttcgtatg ccggtttttt tctttatatc cggctatttg attcgccgct atatcgacag
    20701 cgtaccgtgg ggaaattgtc tcgataaacg gatctggaac atcttctggg tgctggcgct
    20761 ttggggcgtg gtgcagtggc tggcgttaag tgcgctgaac cagtggctgg cacctgagcg
    20821 cgatttaagc aatgcctcca atgccgctta tgccgattcc accggtgagt tcctgcacgg
    20881 gatgatcacc gccagcacca gcttatggta tctgtatgcg ttaattgtct atttcgtggt
    20941 atgtaaaatt tttagccgtc tggcgctgcc actattcgcc ttgtttgtac tgctgagtgt
    21001 ggcggttaat ttcgttccca cgccgtggtg gggaatgaac agtgtgatac gcaatttgcc
    21061 ttattacagc cttggcgcat ggtttggcgc aacaataatg acctgtgtta aagaggtgcc
    21121 gttgcgccgc catctgctga tggcttcttt gcttaccgtt ctggcggtcg gtgcctggtt
    21181 gtttactatc tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga agctgtttta
    21241 tcagtacgag caacgattcg gtatgcgctc caccagcctg ctgaatgtga ttggctccaa
    21301 caccattgct atctacacca cccatcgcat tctggttgaa atattcagct taactctgct
    21361 tgcgcaaatg aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc tgctggttta
    21421 tccctttgtc agtttgttca tctgtactgt tgcgggcttg ctggtaagaa aactttcaca
    21481 gcgtgcattc agcgatctgt tgttctcccc gccttcactg cccgctgccg tcagttactc
    21541 tcgttaaacc atcgccccag ccggggcgat tcaatttgct aaaccatgtc gattacggat
    21601 aatgaacagc attgcttatc agagatatgc ccccatatgt tgaggcatat cctaacgaga
    21661 atctgacaac cgttatgcct gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc
    21721 agcgtctgct gggcgagtta accggcgcag cctgtgcaac gctggtagcg gaaattgccg
    21781 aacgtcacgc cggtccggtg gtactcattg caccagatat gcaaaatgct ctgcgtttgc
    21841 atgatgaaat cagccagttc accgatcaga tggtgatgaa tctggcggac tgggaaactc
    21901 ttccctacga cagtttttcg cctcatcagg acattatctc ctcgcgcctt tccacccttt
    21961 accagctacc gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc
    22021 gcgtttgccc acacagtttt ctccacggtc atgcgctggt gatgaaaaaa ggtcagcgcc
    22081 tgtcacgaga tgcattacga acccaactgg acagcgccgg ttatcgccat gttgaccagg
    22141 tgatggagca cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc ccgatgggga
    22201 gtgagctgcc ttatcgtctt gatttctttg atgatgaaat cgacagcctg cgggtgtttg
    22261 acgtcgacag ccagcgcacg ctggaggaag tagaagcgat caatctgctg cccgcgcacg
    22321 aatttccgac cgataaagcg gcaattgaac tgttccgcag ccagtggcgc gataccttcg
    22381 aagtgaagcg cgatccggaa catatttacc agcaagtgag taaaggcaca ttacctgccg
    22441 ggatcgagta ctggcagcca ttgttcttca gcgaaccact gccgccgctg ttcagttatt
    22501 tccctgccaa taccttgctc gtgaatactg gcgatctgga aaccagtgcc gaacgtttcc
    22561 aggctgacac gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc ccgctgttgc
    22621 caccacaatc gctctggctg cgggtggacg agctcttctc agagctgaaa aactggcccc
    22681 gagtgcagct aaaaactgaa catttaccga caaaagccgc gaatgccaat ttaggtttcc
    22741 agaaactgcc agacctggcc gttcaggcgc aacaaaaagc gccgctggat gcgctgcgta
    22801 agttcctcga gactttcgac ggtccggtgg tgttctcggt agaaagtgaa ggtcgccgtg
    22861 aagcgctggg tgaactgctc gcacgaatta aaattgctcc gcaacgcatt atgcgtcttg
    22921 atgaagccag cgaccgtggg cgttatctga tgattggcgc tgccgaacat ggttttgtcg
    22981 ataaggtgcg taatctggcg ctgatctgcg aaagcgatct gctcggtgaa cgtgttgccc
    23041 gccgtcgtca ggattctcgc cgcaccatca accccgatac actgatccgt aaccttgcgg
    23101 agctgcatat tggtcagccg gtggtccatc tggagcacgg cgtcggtcgc tatgccggaa
    23161 tgaccacgct ggaagctggc ggcattactg gcgagtacct gatgctcacc tatgccaacg
    23221 acgccaaact gtatgttccg gtgtcgtcac tgcatctgat tagccgttac gcaggtggcg
    23281 cggaagaaaa cgccccgctg cataaacttg gcggcgatgc gtggtcacgc gcgcggcaga
    23341 aagcggcgga aaaagtgcgt gatgtggcgg cggaattgct ggatatctac gcgcaacgcg
    23401 ccgccaaaga gggcttcgcg tttaaacacg atcgtgagca gtatcagttg ttctgcgaca
    23461 gcttcccgtt tgaaaccacg ccggatcagg cgcaggccat taatgcggta cttagcgaca
    23521 tgtgtcagcc gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag
    23581 aagtggcgat gcgcgccgct ttcctggcag tagataacca caagcaggta gcggtgttgg
    23641 tgcctaccac ccttcttgcg cagcagcatt acgacaactt ccgcgaccgt ttcgccaact
    23701 ggccggtacg tatcgaaatg atctcccgtt tccgcagcgc caaagagcag acgcaaatcc
    23761 ttgcggaagt ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa ctgctgcaaa
    23821 gtgacgtcaa gtttaaagat ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg
    23881 tgcgtcataa agagcgcatt aaagcgatgc gcgcgaacgt ggatattctg acgcttactg
    23941 caacaccgat cccacgtacg ctgaatatgg caatgagcgg aatgcgtgac ctgtcgatta
    24001 tcgccacgcc gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag tatgacagcc
    24061 tggtggtccg ggaggcgatc ctgcgtgaaa ttttgcgcgg aggacaggtt tattatctct
    24121 acaatgatgt ggaaaacatt cagaaagccg ccgaacggct ggcagaactg gtgcctgaag
    24181 cacggattgc catcggtcac gggcagatgc gcgagcgcga actggaacgg gtgatgaatg
    24241 atttccatca tcaacgtttc aacgtgctgg tttgtaccac cattatcgaa accgggatcg
    24301 acatcccgac agccaacacc attatcattg aacgtgcgga tcacttcggt ctggcgcagc
    24361 tgcaccagtt acgcggtcgc gtcggacgtt cacatcatca ggcatatgca tggctgctga
    24421 cgccgcatcc aaaagcgatg actaccgatg cacaaaaacg tcttgaagcg attgcctcgc
    24481 tggaagatct cggggcaggt tttgcgctgg caacgcacga tctggagatt cgcggcgcgg
    24541 gtgaactgct tggcgaagaa caaagcggct caatggaaac catcggtttc tcgctgtata
    24601 tggagttgct ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg tcgctggaag
    24661 atctcaccag ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt
    24721 tcatccctga cgtgaacacg cgtctgtcgt tctacaaacg tattgccagc gccaaaacgg
    24781 aaaacgaact ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg ctgccagatc
    24841 cggcgcgtac cctgctggat attgcccgtc tgcgccagca agcgcagaaa ctggggatca
    24901 ggaagctgga aggtaatgag aaaggcgggg tgatcgaatt tgccgagaag aatcacgtta
    24961 atccggcctg gttgattggt ttgctgcaaa aacagccgca gcattaccgc cttgatggtc
    25021 cgacacgcct gaaatttatt caggatttga gtgagcggaa aacgcgtatc gaatgggtac
    25081 gccagtttat gcgtgaactg gaagagaacg cgatcgctta atgtaaatcg ggggcgtttc
    25141 tgcgccccca tctgcaacat ttacaaattc tttgcacttc cctgcactat ccgacactgt
    25201 caccatccat aattcaggct tatctgttta ttacaataac cttatattta ttatggattt
    25261 ttggtgatga tcaaaacgcg tttttctcgc tggctaacgt tttttacgtt cgccgctgcc
    25321 gtggcgctgg cgctaccggc aaaagccaac acctggccgc tgccgccagc gagcagtcgt
    25381 ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct ggaagccatc
    25441 gccaaaaaat ataacgtcgg ctttctcgct ctgttacagg ctaaccccgg cgttgatcct
    25501 tacgtaccgc gcgcgggcag cgtgttaacg atcccgttgc aaaccctact tccagacgcg
    25561 ccgcgcgaag gcattgtgat caacattgcg gagctgcgtc tctattacta cccgccgggt
    25621 aaaaattcgg taaccgtgta tccaataggt attggtcagt taggtggtga cacgctgaca
    25681 ccgacaatgg tgaccacagt ttcagacaaa cgtgcaaacc caacctggac gccaacggca
    25741 aacatccgcg cccgttataa agcacaggga attgagttgc ctgcggtagt gccggctgga
    25801 ccggataacc caataggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg
    25861 cttcacggta cgaacgccga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt
    25921 ctgcgggatg acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat
    25981 atcatcaaca ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta
    26041 catcagccgc tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat
    26101 agcgcaatgc aatcatttaa agatgcagca caaactgacg ctgaagtgat gcaacatgtg
    26161 atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact
    26221 ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga aaaaatcagc
    26281 agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg
    26341 tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt
    26401 gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg atagtaccga
    26461 ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagttg gcaaatgaca
    26521 tggagcttaa aatcgccgca gcgatgaggg tttttacgtt tttcataata gtggccttat
    26581 gcagatgaat gacgtcgcaa gaaattactt aacgatcgat aactaaatcg atagatgtga
    26641 tctggatcac atacaagata ttttttataa caatcattaa ttaatttaaa aatctttaaa
    26701 tttcagttaa atagaaagca attattttta tgggatctgg actggtggac agaggtgatt
    26761 atttttataa taacctttca ttcacagtca ccaggtacaa cggcatcatg gcaactgact
    26821 caacacaatg tgtaaaaaaa agccgtggcc gcccaaaagt gttcgacagg gatgccgcgc
    26881 ttgataaggc catgaaattg ttctggcaac acg
//
LOCUS       DADLSB010000038        26847 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.038, whole genome shotgun sequence.
ACCESSION   DADLSB010000038 DADLSB010000000
VERSION     DADLSB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.038"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(33..1220)
                     /gene="eutG"
                     /locus_tag="JN331_001621"
     CDS             complement(33..1220)
                     /gene="eutG"
                     /locus_tag="JN331_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HAY7259794.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQLRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSERKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDSD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(1210..2046)
                     /gene="eutJ"
                     /locus_tag="JN331_001622"
     CDS             complement(1210..2046)
                     /gene="eutJ"
                     /locus_tag="JN331_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HAY7259795.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVM
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(2057..3460)
                     /locus_tag="JN331_001623"
     CDS             complement(2057..3460)
                     /locus_tag="JN331_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HAY7259796.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECRSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(3472..3759)
                     /gene="eutN"
                     /locus_tag="JN331_001624"
     CDS             complement(3472..3759)
                     /gene="eutN"
                     /locus_tag="JN331_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HAY7259797.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(3866..4159)
                     /gene="eutM"
                     /locus_tag="JN331_001625"
     CDS             complement(3866..4159)
                     /gene="eutM"
                     /locus_tag="JN331_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAY7259798.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(4198..5214)
                     /gene="pta"
                     /locus_tag="JN331_001626"
     CDS             complement(4198..5214)
                     /gene="pta"
                     /locus_tag="JN331_001626"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7259799.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLAHAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGYKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRTLTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEMNRESSLQ
                     TLVE"
     gene            complement(5211..6014)
                     /gene="eutT"
                     /locus_tag="JN331_001627"
     CDS             complement(5211..6014)
                     /gene="eutT"
                     /locus_tag="JN331_001627"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HAY7259800.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQLVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLGHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(6011..6712)
                     /gene="eutQ"
                     /locus_tag="JN331_001628"
     CDS             complement(6011..6712)
                     /gene="eutQ"
                     /locus_tag="JN331_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HAY7259801.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(6687..7166)
                     /gene="eutP"
                     /locus_tag="JN331_001629"
     CDS             complement(6687..7166)
                     /gene="eutP"
                     /locus_tag="JN331_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY7259802.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(7179..7514)
                     /gene="eutS"
                     /locus_tag="JN331_001630"
     CDS             complement(7179..7514)
                     /gene="eutS"
                     /locus_tag="JN331_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY7259803.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(7807..10086)
                     /gene="maeB"
                     /locus_tag="JN331_001631"
     CDS             complement(7807..10086)
                     /gene="maeB"
                     /locus_tag="JN331_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY7259804.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            10375..11325
                     /gene="tal"
                     /locus_tag="JN331_001632"
                     /pseudo
     CDS             10375..11325
                     /gene="tal"
                     /locus_tag="JN331_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
     gene            11345..13348
                     /gene="tkt"
                     /locus_tag="JN331_001633"
     CDS             11345..13348
                     /gene="tkt"
                     /locus_tag="JN331_001633"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7259805.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     NPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(13451..14494)
                     /locus_tag="JN331_001634"
     CDS             complement(13451..14494)
                     /locus_tag="JN331_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY7259806.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSSELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(14620..15195)
                     /gene="nudK"
                     /locus_tag="JN331_001635"
     CDS             complement(14620..15195)
                     /gene="nudK"
                     /locus_tag="JN331_001635"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY7259807.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(15263..17242)
                     /gene="aegA"
                     /locus_tag="JN331_001636"
     CDS             complement(15263..17242)
                     /gene="aegA"
                     /locus_tag="JN331_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY7259808.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            17448..19160
                     /gene="narQ"
                     /locus_tag="JN331_001637"
     CDS             17448..19160
                     /gene="narQ"
                     /locus_tag="JN331_001637"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HAY7259809.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGKPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAISCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEGKGNT
                     PAEV"
     gene            19362..22475
                     /gene="acrD"
                     /locus_tag="JN331_001638"
     CDS             19362..22475
                     /gene="acrD"
                     /locus_tag="JN331_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY7259810.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGID
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(22574..22633)
                     /gene="ypfM"
                     /locus_tag="JN331_001639"
     CDS             complement(22574..22633)
                     /gene="ypfM"
                     /locus_tag="JN331_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY7259811.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            23014..23370
                     /locus_tag="JN331_001640"
     CDS             23014..23370
                     /locus_tag="JN331_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY7259812.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23374..24501
                     /gene="dapE"
                     /locus_tag="JN331_001641"
     CDS             23374..24501
                     /gene="dapE"
                     /locus_tag="JN331_001641"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY7259813.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            24529..24729
                     /locus_tag="JN331_001642"
     CDS             24529..24729
                     /locus_tag="JN331_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY7259814.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(24839..25537)
                     /gene="ypfH"
                     /locus_tag="JN331_001643"
     CDS             complement(24839..25537)
                     /gene="ypfH"
                     /locus_tag="JN331_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7259815.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(25611..>26847)
                     /locus_tag="JN331_001644"
     CDS             complement(25611..>26847)
                     /locus_tag="JN331_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7259816.1"
                     /translation="VDEAAAIPAPLLHQLVLRFPRTLLTTTVQGYEGTGRGFLLKFCA
                     RFPHLHRFELQQPIRWAQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWRSE
                     PETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAGENEIAGALWLVDEGGLSQE
                     LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIA
                     GALQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDA
                     GKQLAEREHYRLRRDAQALAQWNGEMLPVDPLNDAILSDDDWLELAGFAFAHRPLLTS
                     LGCLLRLLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLVRQREEAAQALFAL
                     NEVRTERLRDRITQWQFFH"
BASE COUNT         6189 a         7386 c         6960 g         6312 t
ORIGIN      
        1 ggataagacg cggcaagcgt cgcatccggc atttattgcg ccgctgcgta caggccgaca
       61 atctgctcca ggctggcggt acgcgggtta ctgcgcagac aaatatcttc cagcgcggcc
      121 tgcgcccatg cgccgtaatg cgcagatgtc gcaccaacat cgcccagtcg tttaccaatc
      181 ccaacttcag caatcagctc acttaccgcg ttaatagcgt cactatcgtc ggatttttta
      241 gttcgcagcg cccgaccaat ctgactaaac cgatcacgac aaaccatccg gttaaactcc
      301 atcaccgttg gcagcaacat agcgttcgcg agaccatgtg gaatatgcag cgccgcgccc
      361 ggctgatgcg ccatcgcgtg gcacaaccca agacccgcac tggaaaacgc cattcccgcc
      421 atacatgaag ccagcaacat gctctcgcgc gcggcaaggt cgtgaccgta gcccaccgct
      481 ttcggcagcg atttgccaat catcgcaatg gcaccaatcg ccagactgtc ggtaaacggt
      541 gtagcgttca gggcgctgta tgcttcaatg gcatgggtta acgcatcaat gccggtcatc
      601 gccgtgacat gcgacggcac accttcggtc aatgcggcgt cgaggatcgc cacatccggc
      661 atcagcgagg catgggctaa cacctgcttg cgctcgctca ccgcgtcgat aatcaccgtt
      721 acattggtgg tttcagagcc ggttccggcg gtagttggaa tggcaatcag cggcaagcgc
      781 agttgcagaa cgctggtttc tgacatctct gccagcgtgc tatccgggtt cgtcaccagc
      841 aacgccacgg ctttcgccgc atccagcacc gagccgccgc caaatgcgat caccccgtca
      901 caacctgact cacgcaactg cgccacggct gcacacacgt cggtaatgca cggttcgccc
      961 accggacatg gccagagcgt catggcgata cctttaacgg ccaggctacg cgtcagcccg
     1021 gcggtcatcc ctgcctgatg caaaaagctg tctgccatca cgaacagatg tttcagccca
     1081 cgcgtttgcg cttgctgtcc gcaactgctc accgagcccg gaccgcaaag cgtcaccggt
     1141 ggaacgctaa atgtttttac ccgttgcaga ttcagggtat cgaacgcctg aaagagcgcg
     1201 gtctgcaatt cattttgcat atagcccctc cgctttctct cgcccactac tggcgatcgc
     1261 cagcggggtc ataaacaaac tgtgctgcgg taaatgcacc tgtaacgccg ggaattgttt
     1321 gcgaaacagt tctgccacgc ccggttgcat acaggagccg cccgccagcc ataaatcagt
     1381 aatgccctgc ccttcaatat gccgggcgac aatgtccgcc attttttcgt acaccggttt
     1441 caccgcaggc caaatctctt caccgtgacc gcgcttgtac tgctccgcct cttccagcga
     1501 aatacggcga tttccggcga gagtcagaga gatgtgatgt ccgccggtgg cttcatccgc
     1561 cgagtacatc actttgccct ttttcacgat ggcaatgccg gtagtgccac cgccgatatc
     1621 cactacaccg gcgttatcca gttgcagtag atccgcgact gcggttggct catccagcac
     1681 atggctcact tccagcccgg cagattccag cacgttgatg gaaatacgcg ggtccgtacc
     1741 tggaggaaat gaagtcgccg catggctaaa acgacgaccg aattgctgct cgagcgtgtc
     1801 gagatggcga cgaacaatgg tgaccgcgcc gaagaaatcc cagacgatgc cgtcgcggac
     1861 cacatcggcc cagtcgaggc ataccgccac cggctgaccg tcgcggtcga caaccatcga
     1921 caccacatcg caggtgccca gatccacccc cagccacagc ggggattccg tcgcggcagg
     1981 cgtctggtta cacagcgttg ccgccgtttg cagacgtggg gtgagccatt gttcgtcgtg
     2041 cgccatcggt tactccttaa acaatgcgaa acgcatcgac taatacacag cgacgcagac
     2101 ggacaaacgt acgcgcgctg gttacccctt caccggttgg cgtggtgatg gtcatggtgg
     2161 tccagccttc cccgcccagt cccagcccgg caatgcacgg tccgttctta acgaaaatgc
     2221 tggtatcaat ggcattcgcc atctggttca tgttctcgat attgcgcgag tgcattgccg
     2281 ccgtgtggtg gcaaccgccc tccagtttca ccgccagcgc aatggcatcc gccacgttgg
     2341 cgacgcgcac gacgggcagc accggcatca tcagttcggt cacggcaaac ggatgttctg
     2401 cagtggtttc cacaaacagc aaacgcgttt cttgcggcac gttcaggccg attgccgccg
     2461 cgattttcgc cgcatcgcga ccaacccagt cacggctgac ggtgcctttt ccgcgctcgt
     2521 cgatattttt cagcaacacc ggttgcagct gctgcgcctg ttctgcggtc agtttcaccg
     2581 cgtgctggcc ttccatcaga cgcatcagtt catcggctac gctatcaaca acaatcagta
     2641 ccttttcgtc ggcacaaatg atgttgttat cgaaagacgc gcctttgacg atggactgag
     2701 cagcacgggc gaggtcagcg gtttcatcca ccactaccgg cgggttgcca gcgcctgcgg
     2761 caatcagacg tttattggtg tgtttacgcg ccgcttctac taccgcttcg ccgccggtca
     2821 ccaccagcag gccgataccc ggaaacttga acaagcgttg cgcggtttcg atatccggat
     2881 ttgccacagt aaccagtaag ttttccggcc cacctgcggc aacaatcgcc tggttgagca
     2941 gcgtaatcgc ccgctgggag acttttttcg ccgccggatg cggggcaaaa atgacgctgt
     3001 tgcccgcggc aatcaggctg atggcgttgt taattacggt tgccgccggg ttagtggaag
     3061 gcgtcaccga agccaccacg ccccagggtg cgttttcaat tagggtcagg ccgttgtcgc
     3121 cagtcagcac ttgcggagag cggcactcaa cgcctggtgt gccgcgcgcc tgagcgacgt
     3181 tttttgcaaa tttatcttca acgcgcccca tgccggtttc actgacggca agttccgcta
     3241 aatctctggc gtgtttttcg cctgcttcac gaatggcagc aatggctaac tggcgcattg
     3301 ccacgctttt taacccttgc tgggcgactt tggctgccgc aacggcgtca tccagggacg
     3361 cgaaaacgcc catctcatga acagcggcgg gcggcgtgtc actgctttgc attttcagca
     3421 gtaccgcttt caccacctgt tcaatatcct gttgattcat gatgttctgc cttatttgtg
     3481 gaaaattacc tgaccgccag acaccacctc atcgacgatg ccaatcacgc acagatcgac
     3541 cggtgacgtt tcgcttttat gcgcctggcg ggcggaactg ccactcacca gcaacaccca
     3601 ctccccggtt cccgcgccaa tattgtcgat ggcgacggca cattgcccgt caggattacc
     3661 ttgtggatca atcatttcca ccatcagcaa tttgtcatgc gccagtccgt gatggcgtac
     3721 ggtacaaaca atttgtccag tgacgactgc cagtttcata cccgcctccg tggcgtattt
     3781 caggtaaaag ctccccctac cctccgcaga aggtaaaatg aaaaaggaga gagcgtgacg
     3841 cccgaatcga cgtcacacag ggtgattaca ggttgctgct atcgcctttc aggccgatcg
     3901 ggaacacttc ttccaggtcg ccgtgcggac gcgggataac gtgtacagat accagctcgc
     3961 cgatacgctg tgccgctgcg gcaccggcat cggtcgccgc tttacaggct gcaacatcac
     4021 cacgaaccat ggcagtacac aggccgccgc caatctgctt aacaccaacc agcttgacgc
     4081 gcgcagcttt caccatcgcg tcagaagcct caatcagtgc aaccaggccc cgggtttcga
     4141 tcattcctaa tgcttccatt gtgctttcct ctttatcagg gtccagaacg ggaccgttca
     4201 ttcaaccagt gtttgtaaac tgctttcgcg gttcatttct gtctgacgcg gcacagctgc
     4261 caccagcgcc agctcgataa tttcctgcac gctacaacca cgagagagat cgtgcatcgg
     4321 cgcggcaagt ccttgtatca atggcccgac ggcacgatat ccgccgagtc gttgtgcgat
     4381 tttgtagcca atatttccgg cttccagcga cggaaaaacc atcacattgg ctttgccctg
     4441 tagcgggctg gcaggcgctt tttgcgccgc cacttccggc acgaaggcgg cgtcaaactg
     4501 caactcgcca tcaaccacca gctttggtgc gcgctcacgg acgatttctg tcgcctgctg
     4561 gacgttggca acgcaggggt gacgggcgct accgttactg gaaaacgaca gcatcgccac
     4621 gcgcggctct tctccggtga gggtgcgcca ggtttcggca ctggcaagcg cgatatccgc
     4681 cagttgcgcc gccgtcggct gtggcaccac gctgcaatcg gcaaagccca acgccggacc
     4741 gctgtactgt ggcagcatca ggaaaatgga tgagagcgtt ttatagcctg gctgcaagcc
     4801 gataatgcgt aatccggcac gcagcacatt cgccgtggaa gagaggttgc ccgcgataca
     4861 gacatccgct ttaccggcgc tgaccattgc tgcggcgaac atcagcggat cggtaagttt
     4921 ttccagcgca tccggcggcg ttttttcgcc cgcgtgggcc agccagcgat gagcaaattc
     4981 ttcccgcatt gcgaggttgc catgcggatc aatcacctgt agcccgtcca tcgccacgcc
     5041 gtgactgagt gcaaactgac gaagttcaaa cggattagcg accagaatgg gcgttgccag
     5101 accttgttga tgtaaatatt gcgcagcttt cagcacacgt tgatctaacg catccggaaa
     5161 aaccactctg gcgggcgctc gcaacgccag ttcacgacaa cgttcaataa tcattgcgtt
     5221 tcccccagtc gttgttggat ctgcttcacc gttagcggcg gcttcgttac gctcaggatc
     5281 atcattacgt agaccgtgct cgaaaggcgg ttcagcgcct gcaagatgtc cgggcgtaat
     5341 acttcaaaac tgcgggtgat aaacacctgc gccgctacgg tttcggtttc acgcactttg
     5401 gtgcgtagca gattgagcaa cgccgcatcg cgtccgtggc tggcttccgg caccagatga
     5461 tcgtgaccaa gataacgcaa cggctgatga gaaagccggt gcagatcttc gtcagaaagc
     5521 ccgacaatcg cctggcagcc cagcggttcc cccagcgcat cggcgcgcat aatgttgcca
     5581 agacgcgaac ggatatccgc cagccacggc tgccacggtt ccgccagttc aatttgcagc
     5641 cacactgcca gcgcaatggt gctgtcgaga accgcgcgaa aacccagacg cggatcgctt
     5701 ttggcgacca ttttttccgc cgacaggtga gtcagcgtat ccggcttttt tgccaccagc
     5761 tggcggcaca gttcgcagca cgcctgcgga tgttcatcgc tactagtcag cccatgaacg
     5821 ggctgcggct gctgctgttc atcgtcaaca aacaggcggc cctgctcgtc aataaacttg
     5881 atgcgcagat ggcggctttc cagcaactcc cgggcagagg gcgtcaggcg actgtccgca
     5941 gggagatgga tctctgcgcc ttcgctgagc gtatggttcg ctcttagcca tgcttcggtg
     6001 atgaaatctt tcatagggat tgccagttag ccggccaggc gacatacagg aatttcacgc
     6061 tggatgtcgt accaaattcg atgctggagc ctttcgggat aaacatcacg tcgcctgctt
     6121 tggcgatcat ggtttcgcct tcgtggcgaa catgcagctc cccttccagc accatgtcga
     6181 tttcgtcgta gttcagggtc cacgggaaga atgcgttctc ccactgcata aaaccggcgg
     6241 ccatgctgct gccgtcgtct ccggtcacaa gatcagtcaa gcccacgcaa tgcggctctg
     6301 cgccgtcgaa acgaccaaac ttgacgctgc tgccatcaat cactttgatg ccgcctttgc
     6361 cggtcaccga tttgaagctc ggctgcattg cgccctgctc cagcgactgt ttttccttca
     6421 tcactttttc catcagctgt gcgaccaggc tttcggtaaa ctggccttcc ggcagctggg
     6481 caatgatggt ttcgcggatg cgctggcttt cggttttgtc ggctggcacg ctggcgggca
     6541 cggacgccgt taccgggatc gattcatcac attcggtgat ggtaaaaccc agtagatccg
     6601 ccacttcacg ggcttccggg gtaataatgc tggcgcgcag aaccacggac attgcctgtt
     6661 cgccgcgcgc gtgtgcttca cgaatatcat tcgctgtgat gagttttttc acctgcttcc
     6721 tcctgtttgg tgagagatgc cagataatcc accagttgct gcacgctttg cgggtcgtgg
     6781 ctgtttagct cgaaaatcgg ctcttcgaaa cccgtctcaa gcaataactt tcgcgttgcg
     6841 gcgacgtcag catctggcat gtccgtttta ctgatgacgg cgatttgccg tttgctgacg
     6901 ccaatatcca gcaacccggc aggcaaccga ctttccgggt catttgcgcc atggacatag
     6961 atcagcatgt ctacatcctg tagcgtggta attaaggcgt gataccagcg gggatggcta
     7021 aaatattcac ccggcgtatc aatatcgccc ttatcattaa attccacggc ctgtgttttt
     7081 ctggcgaggg tataattccc ctgcaacgca ttaaataacg ttgtttttcc tgccccgacg
     7141 ctacccacaa acgcaattcg tttcatgaat gcctcgtatt aacttttggt catttcacaa
     7201 agcgtataat ttaataagcg acctaaaccg ctgacggttt gtgataacgc ttcttctact
     7261 gcgcccaccg agccataaat caccaatgca ccgctgaagc ggtcgagaaa accgatatgc
     7321 acatcagcgg ctttcagcgc taaatcaccg gcgatcatcg ccgtttcgcc gggagttagc
     7381 gtcataatgc caattgcgcc cgcatccgga acgccgattt tcttcgcaag ttcctcaccg
     7441 ggatgcgcga tgagatgcgc cagtgtgacc tgtttgccgg gcacaaattc ctgaatgatg
     7501 cgttctttat ccatgagtcg ccgcctgtga aaaactttcg tgtatcgtga caatttttcg
     7561 tcagagtaaa taacaaaatt cggcaagcgc gtttaaaagg tgaggtagat cactaagaat
     7621 ggagagatat ttactgttat gaataaactt tcatatgcga cgcatgaata taaataaaat
     7681 aatatcgaga tattttatat actgattttt ttattattcg caaactcagt aataagcttt
     7741 gctaatatat ttcaggaaaa ccgcttaccc tgaatattca gggtaagcgt gagagttaag
     7801 aatgaattac agcggttggg tttgcgcttc aaccacggcc agcgccacca tattgacgat
     7861 acgacgcacc gatgcgatcg gcgttaacac gtgaaccggt ttcgccacgc ccatcagcac
     7921 cggaccgaca gtcacacctt ccgagctgga aacgcgcagt aagttgtaac taatgcgggc
     7981 agcttccatg ttcggcatca ccagaatatt ggcagaacct ttcaaagggc tgtccggcat
     8041 acggtcgttg cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac catcaatcat
     8101 cagatctggc gcacgatccc tgaccagttc cagcgcctga cgcattttgc tcgacgacgg
     8161 gcagtcagaa gaaccaaagt tggagtgcga caacagagca acgcgcggct caataccaaa
     8221 acgacggaca gtttctgccg ccatcaaggt gatctccgcc agctcttctg cgtctggttc
     8281 gtcattgacg taggtatcgg caataaaggt gttaccactc ggcagcagca gcgcgttcat
     8341 tgcacctgcg gtgtgaacgc catcgcgata accaaagaca tttttcacca cgctaaaatg
     8401 ttcatgataa tcgcccaccg taccgcagat cattgcatcg gcttccccac gctgaaccat
     8461 gatcgcgccg atcactgtcg ggttactgat cagcgcacgc tgcgcctgtt cctgagtgac
     8521 accgcgacgc ttcatgatct ggaagtattc ggtccagtac tctttaaagc gcggatcgga
     8581 ttcgttattg acgatttcaa aatcaacgcc cgctttgatc tgcaagccca gtttctgaat
     8641 gcgcatttcg atcacgttcg gacgaccgat aaggatcggt ttcgccagtc ccagcgttac
     8701 cagttcctga gtcgcatgca gaacgcgcgc ctcttcccct tccggcagaa ctacgcgctt
     8761 cggcgctttg cgagcctggg agaaaatcgg cttcataaac aggttggttt tgtaaacgaa
     8821 ctcagtcagc ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga
     8881 ctccatcgcc gcttttgcga ccgcaggagc gatcttaacg atcaagcgcg gatcaaacgg
     8941 ttttggaatg atgtattccg gaccaaagct cagatcctga acgccatacg ctgaagccac
     9001 cacttcactc tgttctgcat gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat
     9061 ctcttcgttg atggcggttg cgccaacgtc cagcgcgcca cggaagatga acgggaagca
     9121 aagaacgttg ttcacctggt tcgggtagtc ggaacggccg gtacagatga tggcatccgg
     9181 acgcacttct ttcgccagcg gcggcagaat ttccggttcc gggttcgcca gcgccaggat
     9241 cattggtgcc cgagccattt tcttcaccat ttcctgggtc agcactttcg ggccggaaca
     9301 gcccaggaaa atatccgcac cttcaatcac atcatcgagg gtacgtttgc cgtcatccac
     9361 caccgcatat gcggctttgg tttccgccat atttggctca cggccctgat agataacgcc
     9421 ttttgaatcg caaaccacga tgttatgttt ttgcagcccc agcgctacca gcaggttcat
     9481 acaggcgatt gctgcggcac ccgcgccgga aaccaccatc cgcacgtcgg agatgttttt
     9541 ctccaccacg cgcaagccgt tgaggatggc ggcagtgctg atgattgccg tgccgtgctg
     9601 atcgtcgtgg aataccggaa tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca
     9661 ttctggcgct ttaatgtctt cgaggttgat gccgccgaag gtcggttcga gcgcggcgac
     9721 gacttcaata aatttgtccg ggtcgagttc atcaacttca atgtcaaata catcaatccc
     9781 agcgaatttc ttaaatagaa cgcccttgcc ttccatcacc ggtttgcctg ccagcgcgcc
     9841 aatgttgcct aaccccagca ccgccgtacc gttagagatc accgcaacca ggttaccgcg
     9901 cgcggtatat ttgtaggctt ttagcgggtc tttttcgatt tcaagacaag gtgcggcaac
     9961 gcctggtgag taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac
    10021 ctggattttc cctggaactg gaaattcatg gaaatcaagt gcactttgtt ttaactggtc
    10081 atccatttgg ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg caacagcccc
    10141 tgcccacaac gtggcgcaca ttattaccct gccggagtct acagactttg agcaagtcca
    10201 aactctcacc attaatataa tgttttggta ataatcctat aacactgatg ttacctgctt
    10261 aatccagcaa taccatgcct gtctgctatg cttttttgat gcgtttcgcg aaatttctca
    10321 gaagtgtgaa ttaacgcact catctaacac tttacttttc aaggagtatt tcctatgaac
    10381 gagttagacg gcatcaaaca gttcaccact gtcgtggcag acagcggcga tattgagtcc
    10441 attcgccatt atcatcccca ggatgccacc accaatcctt cgctgttact caaggctgcc
    10501 ggattatcac aatatgagca tttaatagac gatgctatcg cctagggtaa aaaaaatggc
    10561 aagacccagg aacaacaggt ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct
    10621 gaaattctca aaatcgtacc cggttgcgtg tcaacagaag ttgatgcacg cctctctttt
    10681 gataaagaaa agagtattga gaaggcgcgc catctggtgg acttgtatca gcaacaaggc
    10741 gttgagaaat cacgcatttt gatcaaactg gcttcgacct gggaaggaat tcgcgcggcc
    10801 gaagagctgg aaaaagaagg tattaactgc aacctgacgc tgctgttttc ttttgcccag
    10861 gcgcgggcct gtgcggaagc aggcgttttt ctgatttcgc cgtttgtagg gcgtatttat
    10921 gactggtatc aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga tccgggcgtt
    10981 aaatcggtgc gcaatatcta cgactactat aagcaacacc actatgaaac cattgtgatg
    11041 ggcgcgagct tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga tcgactgact
    11101 atcgcaccga atttgttgaa ggagctgcag gaaaaagttt cgccagtggt acgtaaatta
    11161 atcccgcctt ctcagacgtt cccacgccca gcacccatga gcgaagcgga gttccgttgg
    11221 gagcacaatc aggatgcgat ggcggtagaa aaactgtctg aaggcattcg tctgttcgct
    11281 gttgatcaac gcaaactgga agatcttctt gccgccaaac tataaaccag ccacggagtg
    11341 ttatatgtcc cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg atgcggtaca
    11401 aaaagccaat tctggtcatc ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct
    11461 gtggaacgat tttcttaaac ataatcctac cgacccaacc tggtatgatc gcgaccgctt
    11521 tattctttcc aacggtcacg cgtcgatgct gctctacagt ttgctacatc tgaccggtta
    11581 cgacctgccg ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa ccccaggcca
    11641 cccggagatt ggctatacgc caggcgttga aaccaccacc ggcccgcttg gacaaggttt
    11701 ggcgaacgcc gtcgggctgg cgatagcaga gcgtacactg gcggcgcagt ttaaccagcc
    11761 agaccatgag atcgtcgatc acttcaccta tgtgtttatg ggcgacggct gcctgatgga
    11821 aggtatttcc cacgaagtct gttcgctggc aggcacgctg ggactgggca agctgattgg
    11881 tttttacgat cacaacggta tttccatcga cggtgaaaca gaaggctggt ttaccgacga
    11941 tacggcaaaa cgttttgaag cctatcactg gcatgtgatc catgaaatcg acggtcacaa
    12001 tccgcaggcg gtgaaggaag cgatccttga agcgcaaagc gtgaaagata agccgtcgct
    12061 gattatctgc cgtacggtga ttggctttgg ttcgccgaat aaagcaggta aggaagaggc
    12121 gcacggcgca ccactggggg aagaagaagt ggcgctggca cggcaaaaac tgggctggca
    12181 ccatccgcca tttgagatcc ctaaagagat ttatcacgcc tgggatgccc gtgaaaaagg
    12241 cgaaaaagcg cagcagagct ggaatgagaa gtttgccgcc tataaaaagg ctcatccgca
    12301 actggcagaa gagtttaccc gacggatgag cggtggttta ccgaaggact gggagaaaac
    12361 gactcagaaa tatatcaatg agttacaggc aaatccggcg aaaatcgcta cccgtaaggc
    12421 ttcgcaaaat acgcttaacg cttacgggcc gatgctgcct gagttgctcg gcggttcggc
    12481 ggatctggct cccagcaacc tgaccatctg gaaaggttct gtttcgctga aggaagatcc
    12541 ggcgggcaac tacattcact acggggtgcg tgaatttggc atgaccgcta tcgccaacgg
    12601 catcgcgcac cacggcggct ttgtgccgta taccgcgacg ttcctgatgt ttgttgaata
    12661 cgcccgtaac gccgcgcgga tggcggcact gatgaaagcg cggcagatta tggtttatac
    12721 ccacgactca attggcctgg gcgaagatgg tccgacgcac caggctgttg agcaactggc
    12781 cagcctgcgc ttaacgccaa atttcagcac ctggcgaccg tgcgatcagg tggaagcggc
    12841 ggtgggctgg aagctggcgg ttgagcgcca caacggaccg acggcactga tcctctcaag
    12901 gcagaatctg gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg ctcgtggcgg
    12961 ttatgtgctg aaagacagcg gcggtaagcc agatattatt ctgattgcca ccggttcaga
    13021 gatggaaatt accctgcaag cggcagagaa attagcagga gaaggtcgca atgtacgcgt
    13081 agtttccctg ccctcgaccg atattttcga cgcccaagat gaagaatatc gggagtcggt
    13141 gttgccttct aacgttgcgg ctcgcgtggc ggtggaagca ggtattgccg attactggta
    13201 caagtatgtt ggtctgaaag gggcaattgt cgggatgacg ggttacgggg aatctgctcc
    13261 ggcggataag ctgttcccgt tctttggctt taccgccgag aatattgtgg caaaagcgca
    13321 taaggtgctg ggagtgaaag gtgcctgatg gtgatcgccg gatgctgtat gttcccctca
    13381 ccctaaccct ctccccagaa gggcgagggg acggtccgtg cgctttttag ggccgcatct
    13441 gcgattttgt ttaacgggtg atccacaacg tgggccaggc gtctggccca tgccagttat
    13501 cgcaggtggg ttctgcggcg taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc
    13561 gcgcctgaat gccacaatcg cttaatccgc gccctttcgc taaggtcacc agttcactcg
    13621 atttctcatc aaatgttgcg ttcatcagtt ccagttcatt cgtctcctga ccgttgttga
    13681 acggcaaacg caaccgaacc gggcgcgagg ctagcggctt tttacgcgac acaatccatg
    13741 ccaaatcaat ggtgttatag gcccctgcct cacagctaat catcatcagt gctttatcat
    13801 cggtcagcgc agtgacattc acctcacgac gtaacggatc aagcgagcag cgcagaccat
    13861 tcatccgcca gtttccataa tccagcaaat cgttacgttc ttcgagtgag agtggcgtcg
    13921 gcgttgggtt aaccaccgcg acctctttca gcgcaggcgc gggcggtacg ctgagaggcg
    13981 gttcgtcccc tttcttgatc cacgcggttt cactgccaac gcgcttttgc tgagcatcaa
    14041 taaacaacaa cgccgctttt aagccgctca gagaaatggt ctgattgccg tcgcgtaagg
    14101 tgatcgcctt cccttcctga attatctgca aaaacgcggt gatggttgcc gtatcatcgg
    14161 ttactaataa ccatggtgaa atccgccact tgtcaccact taatgccaac ggctcaccat
    14221 ctaatagcag ccgtggcgct atctcccctt cagacgcatc cggcgacttc aacccaccgc
    14281 gctcaatacg taaaacggca tcggtatgcg ccccagcgct gcggctcagg gtcatcacca
    14341 gtccattatg atcgcccgta ttgcgcgcca cacaaaaatt ttgattatta caggtgacct
    14401 gccagtcgga aaacgcccgt tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag
    14461 cgaaaaaaaa gagaaggaaa atgcgatagc gcatggacgg tacgacccca gaagttcaaa
    14521 caaacagcac aagtcgtatc ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga
    14581 ttgagccaat aataaacaac gaaatattat tgttattttt cagtccatta aatgtgacat
    14641 ttgcaaatag ttaagcaata acaccgtctt accgtcacgt atctcgccgg ttttgatcat
    14701 ctccagcgcc tggctgaacg gcagctcgag cacttcaata tcttcatctt caacaccgcc
    14761 accggcgtta gcgcgctgat tgtcactgta ttcggcgata aaaaagtgga ttagctcagt
    14821 cacaccgcca ggcgacatat acagttcaaa taatttgcgc acttcgccca cttcatagcc
    14881 cgtctcttca atcgcttctt tacgaatgca cacttccggt tcgtcgttat ccagcagccc
    14941 ggcgcaggtt tcaatcagct gcccgctttc attgccatta acccaggtag cgacacggaa
    15001 ctgacgaatc agaaccacgc tctttttctt cgcgttgtac aggaggatcg tcgcaccatt
    15061 accgcgatcg taaacttcac gtttatggcg gataacttcg ccatctttgc gcgtgagatc
    15121 gtaagtaatg ttgtgcaggg tgaaatagtt atcggagaga attttgtctt taatgagggt
    15181 gatttgttgc gtcataccga ctccacagcg cgaaatgaac aattatatta cgctgtgaag
    15241 tcgggtttgt ctgcgcaggc tatcagtgag atttgactga ttttaccccc agccagtcaa
    15301 taatcccctg tgccgcatga cgtccttctg ccattgcggt aaccaccaga tccgcaccac
    15361 gcacggcgtc gccaccggcg aagattttcg gattggtggt ctggtaacgg tactggcttt
    15421 ccacatccgc gatgatgcgg ccccatttgt ctaccgttac accgtgcgac tccagccacg
    15481 gcatcccgtg tggattgaag ccaaacgcca taatcaccgc atcggctggc atgacaaatt
    15541 cactgccctc aatcggtaca ggtcgccgac gcccctgggc atccggctcg ccaagacgcg
    15601 tgcgcaggaa acgaattccg caaacgtgac cttgctcatt cagctcaagc gccaccggct
    15661 ggacgttaaa ttcgaagttg gccccctctt cgcgggcgtt cttcacctct ttcttcgagc
    15721 ctggcatgtt agcttcatca cgacgataag cgcaggtgac gttactcgcg ccgtggcgca
    15781 gcgcagtacg cacacagtcc atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc
    15841 cggcggtatt gataaacggc tcttccggta gctcttcgag ccccatcacc tgtttagtgt
    15901 tggcaatgag gaacggcagc gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg
    15961 ctttcatgga acggtaagtg cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg
    16021 aatccaaaga gacatcttta cccacttcac aattgagttc gaagtgaatc cccatcgcgc
    16081 tgaagatttc ccgacggcgt gccagcaggg atttatccag tttgaaagaa ggaatgccga
    16141 aagtgagcaa gccaccgatt tctggatggc gatcgtacac cgtcaccgcc acgccattgc
    16201 gggtcagaac atccgcacag gccagccctg ccggacctgc cccgataatc gccacccgct
    16261 tgtccacttt ggtgacatga cttaagtcag gacgccaacc tttcgccaac gcctgatctg
    16321 aaatatagcg ttcaatgttg ccgatagtta ccgcgccgtg ctcatcgcga atagtacagg
    16381 caccttcaca caaacggtct tgcggacaaa cgcgtccggt aatttccggc agggtgttgg
    16441 tctggtgaga aagctcgacg gcggcgtcga tgtttccggc tttcaccagt tcaatccact
    16501 gcggaatatg gttatgcagc gggcaggtcc attcacaaac gctgtgctcg ccgaacttaa
    16561 ggcagcgcga ggcttcccgt tgtgcctggt cggcgcgaaa tggcagataa atttcatcaa
    16621 aaccggtttt gcgcgcttca atcgccagtt tatccggctc gccacgcgcg ggcgttgcct
    16681 gcatttgttc gactttactc attaccggca tttcttgtgc agcggtactg gcatgccacg
    16741 gttgatgctc ctgacgtgcg gtgcgcaagc ggcgggattt cgccatgccg gagagtgcga
    16801 cgtcagtgac cagttgcagc gcgtccgccg ggcaattctc gacacaggca ggaccgtttt
    16861 cgcgccctgc acaaaggtcg catttatgcg ccgtggcttt tacttttcct gccgcgacgg
    16921 gtgtcaggac gatttgcatc gtaccaaacg gacaggccac cacgcaggat ttacagccaa
    16981 tacacttttg ctgattgacc tgaatgctgt catcaacgtg gctgattgcg ccattagggc
    17041 agctacgggc gcagggcgca tcttcacaat ggtgacaggt cactgcacta cgttgctgct
    17101 gatgtttgat aaccgtaatt cggggatgaa aatggtgttg gctcaggaca tgttgctcat
    17161 cattgtgagc catgacacag gcgatttcac aagcatgaca acccagacat tgctgactgt
    17221 tggccataat aaaacgattc ataacgacct tcttttttgg ttgtaaaaac cttattcttt
    17281 atatgagtgt tgttattacc cgacttacag gggaaccggc aatgttcatg tgcccagaat
    17341 aagtaactat ttcgctataa actgtggcag atcaaataat cccatcactg actaaattgc
    17401 gtttcagcga actggaacat taatgatttt ttgtggagaa gacgcgtgtg attgttaaac
    17461 gacccgtctc ggccagtctg gcccgggcct ttttttacat tgtgctgctg tcgattcttt
    17521 ccacgggtat cgctctgcta actctggcga gcagtttgcg cgacgctgag gctatcaata
    17581 ttgccggatc gctgcgtatg cagagttacc gcctgggcta cgacttgcaa agtggcagtc
    17641 cacaactcaa tgcacatcgc cagttgtttc agcaggcact gcattcaccg gtattaacca
    17701 acctcaacgt ctggtatgtg ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca
    17761 actggctgga gatgaataat cggctcagca agggcgattt gccgtggtat caggccaata
    17821 ttaataatta tgttaatcag atagacctgt tcgtgctggc tttacagcac tatgctgaac
    17881 gcaaaatgct gctggtggtg gcgatttccc tggctggcgg tatcggtatt ttcacgctgg
    17941 tcttttttac tttgcgccgc atacgccatc aggtggttgc cccgctgaat cagctggtta
    18001 ccgccagtca gcgtattgaa cacgggcagt tcgactcgcc gccgctggat accagtctgc
    18061 cgaatgagct tggtctgctt gcaaaaacct ttaaccaaat gtcgagcgag ctgcataaat
    18121 tgtacctttc gctggaggcg tcagtagaag aaaagacccg cgatctccac gaggccaagc
    18181 gtcgtctgga ggtgttgtat cagtgttccc aggcgctgaa caccagccag attgatgtgc
    18241 attgtttccg ccatattttg cagattgttc gcgacaatga agcagctgaa tatctggagc
    18301 tgaacgttgg ggataactgg cggattagcg aagggaagcc aaaccctgag ctgccaatgc
    18361 agattttacc ggtgacaatg caagagacgg tttacggcga actgcactgg caaaatagtc
    18421 acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc gatgctggga cgcggtttgt
    18481 actttaatca ggcgcagaag cattttcagc agttattgtt gatggaagaa cgtgcgacca
    18541 tcgcccgcga attgcacgac tcgctggctc aggtgctttc ttacttacgt atccagttga
    18601 cgttactgaa gcgttcgata ccggaagata acgccactgc acaaagtatc atggccgatt
    18661 tttcccaggc gttgaatgat gcttatcggc agttacgcga gctgttgact actttccgcc
    18721 tgacgctgca gcaggcggat ctcccctccg cgttgaggga aatgctggat acgttacaaa
    18781 atcaaaccag cgctaaactg accctcgact gccgtctgcc aaccctggca ctggatgcgc
    18841 aaatgcaggt gcatttgttg caaattattc gcgaagcggt gctgaatgcg atgaagcacg
    18901 ccaacgccag cgaaatcgcc atcagttgcg tcaccgcgcc ggacggcaat cacacggttt
    18961 atatccgtga taacgggatt ggtatcggtg aaccgaaaga acccgaaggt cattatggac
    19021 tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac gctgactttt tcacaacctt
    19081 ccggcggcgg cacgttagtg agtattagct ttcgctccgc agagggggag gggaagggga
    19141 acacgccggc tgaggtgtaa ataattaccc tcacaccggc cctctccctt taaaggcgag
    19201 agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt cagcaatctc atgataattt
    19261 acattaactc ctttttttct ccacgattgg ctcgtacctt gccgctacag tgaagcaagt
    19321 caagcctaca acaatacgca gaaacacgag gtcctctttt aatggcgaat ttctttattg
    19381 atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg tctgacaggt accctggcga
    19441 ttttttcatt gcccgttgaa caataccccg atctcgcgcc accgaatgtg cgagtgaccg
    19501 ctaactatcc cggcgcatcg gcccagacgc tggaaaacac cgtgacccag gttatcgagc
    19561 aaaatatgac cggcctcgat aatctgatgt atatgtcatc tcagagcagt ggcaccggtc
    19621 aggcatctgt cactttaagt tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag
    19681 tacaaaacca gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg
    19741 tgacggtgcg taaaaccggc gataccaaca ttctgaccat tgccttcgtc tctaccgatg
    19801 gctcgatgga taaacaggat attgctgatt atgttgccag taatattcag gacccgttaa
    19861 gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc gcaatattcc atgcgtatct
    19921 ggctggaccc ggcgaaactc aacagtttcc agatgacggc taaagatgtc actgatgcca
    19981 ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg tggtacacct tccgtcgata
    20041 agcaggcgct caacgccacc attaacgccc agtcactgct gcaaacacca gaacagttcc
    20101 gcgatatcac cttgcgggtg aatcaggacg gctcagaggt aaggctgggc gatgtcgcca
    20161 ccgtcgaaat gggggcggag aaatacgatt atcttagccg cttcaatggt aagccagcct
    20221 ccgggttggg ggtaaaactg gcctccggcg caaacgaaat ggcgacagcg gagctggtgc
    20281 tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg actggaatac aaggtggcgt
    20341 atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag
    20401 ctatcgctct ggttttcctc gttatgtatc tgttcctgca aaacttccgc gccacgctga
    20461 tacccactat cgccgtgccg gtggtgttga tgggaacctt ctccgtactt tacgccttcg
    20521 gttacagcgt caacacctta accatgttcg cgatggtgct ggcgatcggt ctgctggtgg
    20581 atgacgccat cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa ggactcactc
    20641 ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg ggcactggtc gggattgcga
    20701 tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct
    20761 atcgccagtt ctctattacc attgttgcgg cgatggtgct gtcagtactg gtagcgatga
    20821 tcctcactcc ggctctgtgt gccacgctac ttaagccact gaaaaaaggt gagcatcacg
    20881 ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg
    20941 aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc
    21001 tgcttggcgg catggtgttc ctgttcctgc gtttgccgac gtcgttctta ccgctggaag
    21061 accggggcat gtttactacc tcggtacaat tgcccagcgg ttcaacccaa caacagaccc
    21121 tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca tgaaaaagac aacatcatgt
    21181 cggtgtttgc caccgttggt tctggccctg ggggtaacgg gcaaaacgtg gcgagaatgt
    21241 ttatccgcct gaaagactgg agcgaacgcg acagtaagac cggcacctcg tttgccatta
    21301 tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc tcgcgttatc gccagcagcc
    21361 caccagcaat tagcggtctt ggtagttctg caggttttga tatggagttg caggaccacg
    21421 ctggagcggg tcacgatgcg ctgatggcag cacgtaatca gttgctggcg ctggcggcgg
    21481 aaaacccgga gctaacccgt gtgcgccata acggcctcga tgacagtccg cagttgcaga
    21541 ttgatatcga ccagcgtaaa gctcaggcgc tgggcattga tatcgacgat attaacgaca
    21601 cactgcaaac cgcctggggt tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga
    21661 agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct gccagatgac atcaatctct
    21721 ggtatgtccg aaataaagat ggcggcatgg tgcccttctc tgctttcgcg acctcacgct
    21781 gggaaacagg ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt gagattgttg
    21841 gggaagccgc accgggggtc agtaccggta cggcgatgga tattatggaa tcgttagtga
    21901 agcagctgcc aaacggcttt ggtctggagt ggacagcgat gtcgtatcag gagcggcttt
    21961 ccggcgcgca ggctccggcg ctgtacgcca tttccttgct ggtggtattc ctgtgtctgg
    22021 cggcattgta tgaaagctgg tcggtaccgt tctcggtaat gctggtcgtg ccgctggggg
    22081 taatcggcgc gctgctggca acctggatgc gcgggctgga aaacgacgtt tacttccagg
    22141 tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg
    22201 ctaacgagat gaaccaaaaa ggccacgacc tgtttgaagc gacgctccac gcctgccgtc
    22261 agcgtttacg cccgattctg atgacctcgc tggcatttat cttcggcgta ttgccaatgg
    22321 caaccagcac gggtgccggt tccggtggtc agcatgcggt gggtactggc gtaatgggcg
    22381 ggatgatttc agctactatt ctggctattt acttcgtgcc gctgttcttt gtgctggtgc
    22441 gccgccgctt cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg acatgccaat
    22501 gtgtcgcctt tttcaacttt ccgattaaga acctgctcag cgggttcttg ctgtttatac
    22561 tttgtctcag gaattactta cgaagcataa cttcgataaa gtctttccag ttccccagtt
    22621 cacgttcaat cataacaacc tctcttataa ttatgggtat tctacggaaa caatataccg
    22681 tggtgaagct aatttactcg attgctgcga tgactacctc cgggggacaa accttatgta
    22741 aatactatgg tcctgcggtg ataatttgta tgtgatacac agcaacattt cgagatattc
    22801 atacggcatc taatactgat ttaattctgg ttaaaataca gacagataac aagatgaata
    22861 ttcttaatgt ttacgttaaa aatgtttaat attatttaat agttgttaat ttgaatactt
    22921 cgataatgtt atattttctg ataatcattt gcaggcaaaa tgttttcacc cttaaatgag
    22981 tatttattct cataaatcga aaaaggattc attatggtta cactttacgg catcaaaaat
    23041 tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca ataacatcga ctatcgtttt
    23101 catgattacc gcgtcgatgg gctggacagc gaattattga acggttttat caacgaatta
    23161 ggctgggaag cgttactcaa cacccgtggt acaacctggc gtaaactgga cgaaactacc
    23221 cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa tgactgaaat gcctgcaatt
    23281 atcaaacgtc cattgctctg cgcgcctggt aagcctatgc tgctgggttt cagtgattcc
    23341 agttatcagc aatttttcca tgaggtgtag tctatgtcgt gcccggttat tgagctgaca
    23401 caacagctta ttcgccgccc ttccctgagt cctgatgacg caggatgtca ggctttgttg
    23461 attgaacgtt tgcaggcgat cggttttacc gttgaacgca tggattttgc cgatacgcag
    23521 aatttttggg catggcgtgg gcaaggtgaa acgttagcct ttgccgggca taccgacgtg
    23581 gtgccgcctg gcgacgccga tcgttggatc aatccgccgt ttgaacccac cattcgtgac
    23641 ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct cgctggcggc gatggtggta
    23701 gcagcagaac gttttgtcgc acaacatccc aaccatacgg ggcgactggc atttctgatc
    23761 acttctgatg aagaagccag tgcccacaac ggtacggtaa aagtcgtcga ggcgttaatg
    23821 gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac cgtcgagtat cgaagtggta
    23881 ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct gcaacctcac cattcatggc
    23941 gttcaggggc atgttgccta cccacatctg gctgacaatc cggtacatcg cgcagcacct
    24001 ttccttaatg aattagtggc tattgagtgg gatcagggca atgaattctt cccggcgacc
    24061 agtatgcaga ttgccaatat tcaggcggga acgggcagta acaatgttat tccgggtgaa
    24121 ctgtttgtgc agtttaactt ccgcttcagc accgaactga ctgatgagat gatcaaagcg
    24181 caggtacttg ccctgcttga aaaacatcaa ctgcgctata cggtggattg gtggctttcc
    24241 gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg cggtcgttaa cgcggttgag
    24301 cactataatg aaattaaacc gcagctactg accacaggcg gaacgtccga cgggcgcttt
    24361 attgctcgca tgggggcgca ggtggtggaa ctcgggccgg tcaatgccac tattcataaa
    24421 attaatgaat gtgtgaacgc tgccgacctg cagctacttg cccgtatgta tcaacgtatc
    24481 atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg aaataagcat ggactggctg
    24541 gctaaatatt ggtggattct ggtgattgtc tttttggtag gcgtcctgct gaacgtgatt
    24601 aaagatctca agcgcgtcga ccataagaaa tttctcgcca acaagccgga gcttcccccg
    24661 catcgtgatt tcaacgataa gtgggacgat gacgacgact ggccgaaaaa ggatcaaccg
    24721 aagaagtaag ctgagtgctc aaccgtaggc cggataaggc gttaacgccg catccggcaa
    24781 tggtgaacga tgcctgatgc gacgctcacg cgtcttatca ggccaggtaa tctgtctttt
    24841 acatcatttc aatcacatca tcatcgccag gcttaccgcc acttagcgct tcgtcgaaat
    24901 aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa ttgcatactg cgattgtcga
    24961 tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc accaccggca ctgattaacg
    25021 cttcctgcgc cgcaactgca tgcgccagat cgataaccgg atcttcacca ccatgaatca
    25081 agtgaattgt cgtggctgta gacgcggtct ccggcaggct cgcatagcgt ccgttaaagg
    25141 caatgacgcg cgaagcaagg cccggttcgg ctttaatgct ctccagcgcc ataatcgcgc
    25201 cttgtgagaa accaattagc gccgtggcgt tagcccctac tccgctctgt ttctgccagt
    25261 agcgtaccgt ttcaataaac gtcggcatga tggcatccac acgcgcctgg cgattatctt
    25321 ccgtaatacc ctgcactgaa aaccactgac gccccgccgg attaccgctc ggctccgcgc
    25381 caccgacact caccaccagc gcatcaggaa acagcggtgc aaaccagctg cctatttccc
    25441 ccattgccac cgggttatcc ccgacaccgt ggaaaagcag taacaactgt tgtgcaggtt
    25501 tatccgggct ttgaacaaca aaatggtcat gtttcatggc gaactcctta actgatgtca
    25561 ttaattttac gccgctgact cacaatgacc atgggagaaa attgaaggag tcagtgaaaa
    25621 aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc gaacctcatt aagtgcgaac
    25681 agcgcctgcg cggcctcttc ccgctggcga accagtaaca tcttgcggcc tgaaagttta
    25741 agtgtggtac ataactgcgc gtcactggcg tttttctgta aacgcccacg cagcgccggt
    25801 aatgccagtt cactggtttg tagcagacgc aataagcaac ctaacgatgt taatagcgga
    25861 cgatgagcga aagcaaaacc ggccagttca agccagtcgt cgtcagaaag gatggcatcg
    25921 tttagtggat caacaggaag catttcgcca ttccactgcg cgagagcttg cgcatcgcga
    25981 cgtaaacggt aatgctcacg ttcagccagc tgtttacccg catcactcat cggtaacagc
    26041 gccatcgccg tatagcaacc gctgctggct tcccgatgat tacccatccg caccagcaca
    26101 aaaccgcagc gttgccagaa acgccataac tccccggtgt aaccaaaact caccgaaaga
    26161 tagtcgaggt catgcgtata ttgcaaagct ccagcaataa gttgccgccc tgtgccttcc
    26221 cgctgacgag ccggatgaac tgctatccgg ctaacccgcc ttccgcgcaa tgtcgccgcc
    26281 agtggattgc tgccgtgcgc cgccagcgac tgggccacca gattaccccg cgggcgacga
    26341 aaacctgccc ataccgcctg actgagttct tgagataatc cgccctcatc caccagccac
    26401 aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa aatgttgccc tggtgcatcc
    26461 atcatccggc gtaaatccag cggcgaggtc cggtagtgcg caccagacaa taactgataa
    26521 acctttaacg gcgtttctgg ctcgcttcgc cataacgttt gttcaaatgc ggaaatgacg
    26581 atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa aaaccagtgc ctcactaacc
    26641 attttttcca gcgggcatcc ctgtgcccag cggatcggct gttgcagttc aaaacggtgt
    26701 aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac gtccggtacc ttcgtagccc
    26761 tgcaccgtag tggttaacaa cgttcgagga aaacgcaata ccagttgatg caacaacggc
    26821 gcaggtatgg ctgcggcttc gtcgacc
//
LOCUS       DADLSB010000039        26624 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.039, whole genome shotgun sequence.
ACCESSION   DADLSB010000039 DADLSB010000000
VERSION     DADLSB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.039"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..375)
                     /locus_tag="JN331_001645"
     CDS             complement(<1..375)
                     /locus_tag="JN331_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259817.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINKFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQ"
     gene            899..2827
                     /gene="thrS"
                     /locus_tag="JN331_001646"
     CDS             899..2827
                     /gene="thrS"
                     /locus_tag="JN331_001646"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY7259818.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2831..3373
                     /gene="infC"
                     /locus_tag="JN331_001647"
     CDS             2831..3373
                     /gene="infC"
                     /locus_tag="JN331_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY7259819.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3470..3667
                     /gene="rpmI"
                     /locus_tag="JN331_001648"
     CDS             3470..3667
                     /gene="rpmI"
                     /locus_tag="JN331_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY7259820.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3720..4076
                     /gene="rplT"
                     /locus_tag="JN331_001649"
     CDS             3720..4076
                     /gene="rplT"
                     /locus_tag="JN331_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY7259821.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            4199..4243
                     /locus_tag="JN331_001650"
     CDS             4199..4243
                     /locus_tag="JN331_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY7259822.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4526..5509
                     /gene="pheS"
                     /locus_tag="JN331_001651"
     CDS             4526..5509
                     /gene="pheS"
                     /locus_tag="JN331_001651"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY7259823.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5524..7911
                     /gene="pheT"
                     /locus_tag="JN331_001652"
     CDS             5524..7911
                     /gene="pheT"
                     /locus_tag="JN331_001652"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY7259824.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQTSLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7916..8215
                     /gene="ihfA"
                     /locus_tag="JN331_001653"
     CDS             7916..8215
                     /gene="ihfA"
                     /locus_tag="JN331_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY7259825.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8316..9296
                     /gene="btuC"
                     /locus_tag="JN331_001654"
     CDS             8316..9296
                     /gene="btuC"
                     /locus_tag="JN331_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY7259826.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9359..9910
                     /gene="btuE"
                     /locus_tag="JN331_001655"
     CDS             9359..9910
                     /gene="btuE"
                     /locus_tag="JN331_001655"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY7259827.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9910..10659
                     /gene="btuD"
                     /locus_tag="JN331_001656"
     CDS             9910..10659
                     /gene="btuD"
                     /locus_tag="JN331_001656"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY7259828.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSVLDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10737..11201
                     /locus_tag="JN331_001657"
     CDS             10737..11201
                     /locus_tag="JN331_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7259829.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11448..12161
                     /gene="ydiV"
                     /locus_tag="JN331_001658"
     CDS             11448..12161
                     /gene="ydiV"
                     /locus_tag="JN331_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAY7259830.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12225..13661
                     /locus_tag="JN331_001659"
     CDS             12225..13661
                     /locus_tag="JN331_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY7259831.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13665..13856)
                     /gene="hemP"
                     /locus_tag="JN331_001660"
     CDS             complement(13665..13856)
                     /gene="hemP"
                     /locus_tag="JN331_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY7259832.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13988..15034)
                     /gene="aroH"
                     /locus_tag="JN331_001661"
     CDS             complement(13988..15034)
                     /gene="aroH"
                     /locus_tag="JN331_001661"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY7259833.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15191..16024)
                     /gene="ppsR"
                     /locus_tag="JN331_001662"
     CDS             complement(15191..16024)
                     /gene="ppsR"
                     /locus_tag="JN331_001662"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY7259834.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16357..18735
                     /gene="ppsA"
                     /locus_tag="JN331_001663"
     CDS             16357..18735
                     /gene="ppsA"
                     /locus_tag="JN331_001663"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY7259835.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18792..20492)
                     /gene="fadK"
                     /locus_tag="JN331_001664"
                     /pseudo
     CDS             complement(18792..20492)
                     /gene="fadK"
                     /locus_tag="JN331_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20495..20788)
                     /locus_tag="JN331_001665"
     CDS             complement(20495..20788)
                     /locus_tag="JN331_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7259836.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20785..22074)
                     /locus_tag="JN331_001666"
     CDS             complement(20785..22074)
                     /locus_tag="JN331_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7259837.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22130..23068)
                     /locus_tag="JN331_001667"
     CDS             complement(22130..23068)
                     /locus_tag="JN331_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY7259838.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYDPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23088..23852)
                     /locus_tag="JN331_001668"
     CDS             complement(23088..23852)
                     /locus_tag="JN331_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY7259839.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24168..25079
                     /locus_tag="JN331_001669"
     CDS             24168..25079
                     /locus_tag="JN331_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7259840.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLINTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(25122..26273)
                     /locus_tag="JN331_001670"
     CDS             complement(25122..26273)
                     /locus_tag="JN331_001670"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7259841.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYSRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(26287..>26624)
                     /locus_tag="JN331_001671"
     CDS             complement(26287..>26624)
                     /locus_tag="JN331_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259842.1"
                     /translation="GSLKTEITDGKLNIVQEGRVKKFIRELPEITFSGKIALERGLDV
                     RYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPELKLMDERLFIDAAM
                     GFVLPEAAH"
BASE COUNT         6580 a         6438 c         6932 g         6674 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag agcagtggaa taacaatttt gtgatatttt
      181 tcgacagttt gcggtaaatt cctgagtaac acagtatgac agcatcgaac ttaagtcaga
      241 ggtatcaagt agggtgatga gctggcgcgc aaatttatta ataggctctg atgaaggtgt
      301 tggtatgtcg ctatcgatgt gcaatctatg aggagactta taatgagttc tgataatgat
      361 atcgttttga ctcataatct attcgcctta tatatatctg gaggttttga ggatgatgat
      421 taattattat cgccagaacg aaatgtgcac gaaaaattgt tataagaatt gtgtcacttt
      481 tatcattgtg atatgaccac ttcgtcatta atctcatgag cgtttaaaat actaccgcca
      541 aatcacgcaa cgaactttgc tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa
      601 cctttcagac gcaccgtgat gttatcagtg gttctttaag cgttttgctg gtgtactcac
      661 tacaaacgaa ttgcgaatcg aatcaatgtg aaacggaaag gtacaatctc ccttttttta
      721 gtgttaacat cgttcaaccg ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct
      781 gattgcgaac caatttagca tttgttggct agatggtttc gcaatgaact gttaataaac
      841 aaatttttct ttgtatgtga tctttcgtgt gggtcaccac tgcaaataag gatataaaat
      901 gcctgttata actcttcctg atggcagcca acgccattac gaccacgctg taagccccat
      961 ggatgttgcg ctggacattg gtccaggtct ggcgaaagcc tgtatcgcag ggcgcgttaa
     1021 tggcgaactg gttgatgctt gcgatctgat tgaaaacgac gcacaactgt cgatcattac
     1081 cgccaaagac gaagaaggtc tggagatcat tcgtcactcc tgtgcgcacc tgttagggca
     1141 tgcgattaaa caactttggc cgcataccaa aatggcaatc ggcccggtta ttgacaacgg
     1201 tttttattac gacgttgatc ttgaccgcac gttaacccag gaagatgtcg aagcactcga
     1261 gaagcggatg catgagcttg ctgagaaaaa ctacgacgtc attaagaaga aagtcagctg
     1321 gcacgaagcg cgtgaaactt tcgccaaccg tggggagagc tacaaagtct ccattcttga
     1381 cgaaaacatc gcccatgatg acaagccagg tctgtacttc catgaagaat atgtcgatat
     1441 gtgccgcggt ccgcacgtac cgaacatgcg tttctgccat catttcaaac taatgaaaac
     1501 ggcaggggct tactggcgtg gcgacagcaa caacaaaatg ttgcaacgta tttacggtac
     1561 ggcgtgggca gacaaaaaag cacttaacgc ttacctgcag cgcctggaag aagccgcgaa
     1621 acgcgaccac cgtaaaatcg gtaaacagct cgacctgtac catatgcagg aagaagcgcc
     1681 gggtatggta ttctggcaca acgacggctg gaccatcttc cgtgaactgg aagtgtttgt
     1741 tcgttctaaa ctgaaagagt accagtatca ggaagttaaa ggtccgttca tgatggaccg
     1801 tgtcctgtgg gaaaaaaccg gtcactggga caactacaaa gatgcaatgt tcaccacatc
     1861 ttctgagaac cgtgaatact gcattaagcc gatgaactgc ccgggtcacg tacaaatttt
     1921 caaccagggg ctgaagtctt atcgcgatct gccgctgcgt atggccgagt ttggtagctg
     1981 ccaccgtaac gagccgtcag gttcgctgca tggcctgatg cgcgtgcgtg gatttaccca
     2041 ggatgacgcg catatcttct gtactgaaga acaaattcgc gatgaagtta acggatgtat
     2101 ccgtttagtc tatgatatgt acagcacttt tggcttcgag aagatcgtcg tcaaactctc
     2161 cactcgtcct gaaaaacgta ttggcagcga cgaaatgtgg gatcgtgctg aggcggacct
     2221 ggcggttgcg ctggaagaaa acaacatccc gtttgaatat caactgggtg aaggcgcttt
     2281 ctacggtccg aaaattgaat ttaccctgta tgactgcctc gatcgtgcat ggcagtgcgg
     2341 tacagtacag ctggacttct ccttgccgtc tcgtctgagc gcctcctatg tgggcgaaga
     2401 caacgagcgt aaggtaccgg taatgattca ccgcgcaatt ctggggtcga tggagcgttt
     2461 catcggtatc ctgaccgaag aattcgctgg tttcttcccg acctggcttg cgccagttca
     2521 ggttgttatc atgaatatta ccgattcaca gtctgaatac gttaacgaat tgacgcaaaa
     2581 actatcaaat gcgggcattc gtgttaaagc agacttgaga aatgagaaga ttggctttaa
     2641 aatccgcgag cacactttgc gtcgcgtccc atatatgctg gtctgtggtg ataaagaggt
     2701 ggaatcaggc aaagttgccg ttcgcacccg ccgtggtaaa gacctgggaa gcatggacgt
     2761 aaatgaagtg atcgagaagc tgcaacaaga gattcgcagc cgcagtctta aacaattgga
     2821 ggaataaggt attaaaggcg gaaaacgagt tcaaacggcg cgccctaacc gtatcaatgg
     2881 cgaaattcgc gcccaggaag ttcgcttaac aggtctggaa ggcgagcagc ttggtattgt
     2941 gagtctgaga gaagctctgg agaaagcaga agaagccgga gtagacttag tcgagatcag
     3001 ccctaacgcc gagccgccgg tttgtcgtat aatggattac ggcaaattcc tctatgaaaa
     3061 gagcaagtct tctaaggaac agaagaaaaa gcaaaaagtt atccaggtta aggaaattaa
     3121 attccgtcct ggtacagatg aaggcgacta tcaggtaaaa ctccgcagcc tgattcgctt
     3181 tctcgaagag ggtgataaag ccaaaatcac gctgcgtttc cgcggtcgtg agatggcgca
     3241 ccagcaaatc ggtatggaag tgcttaatcg cgtgaaagac gatttgcaag aactggcagt
     3301 agtcgaatcc ttcccaacga agatcgaagg ccgccagatg atcatggtgc tcgctcctaa
     3361 gaagaaacag taaggccttc aagtagcact gtctgtggag cctttgggct tcacaggcat
     3421 tgttcgcctt tgtttcgttt attaacaatg cgaagtggaa gttattaaaa tgccaaaaat
     3481 taagaccgta cgcggtgctg ctaagcgctt caaaaaaacc ggtaaaggtg gttttaagca
     3541 caagcacgct aacctgcgtc acattctgac taaaaaagcg accaaacgta aacgtcacct
     3601 gcgtccgaaa gccatggttt ccaaaggcga tctgggcctg gtaatcgcgt gcctgccgta
     3661 cgcataagcc gttaacgttt ttaacttttt aattagaata tagatacagg agagcacata
     3721 tggctcgcgt aaaacgtggt gttattgcac gcgcacgtca caagaaaatt ttgaaacaag
     3781 ctaaaggcta ctacggtgcg cgttctcgcg tataccgcgt tgccttccag gctgttatca
     3841 aagctggtca gtatgcttac cgtgaccgtc gtcaacgtaa gcgtcagttc cgtcaactgt
     3901 ggattgcgcg tatcaacgca gcagcacgtc agaacggtat ttcttacagc aaattcatca
     3961 atggcctgaa aaaagcctct gttgaaatcg accgtaagat cctggctgat atcgcagtat
     4021 tcgacaaagt agcgttcacc gctctggttg aaaaagcgaa agcagctctg gcataagcca
     4081 gttgaaagag ggagctagtc tccctctttt cgtttcaacg ccatcaaaac attgactttt
     4141 atcgccgtag ccttttcaat aaaggtcttt tgaagagtac caaaaggtaa cgcaagcaat
     4201 gaatgctgct attttccgct tcttttttta ctttagcacc tgaatccagg aggctagcgc
     4261 gtgagaagag aaacggaaaa cagcgcctga aagcctccca gtggaggctt tttttgtatg
     4321 cgcgttttga aaaataaaaa gccagtctat caggcgtaat tgtcgcagcc agtctggaca
     4381 cggccagtgc ggagaaaccg gagcgtactt aagtacgtga ggatttcgag cactgcccgg
     4441 gaccaaaatg gcaagtaaaa tagcctgatg ggataggctc taagtccaac gaaccagtgt
     4501 caccactgac acaatgagga aaaccatgtc acatctcgca gaactggttg ccagtgcgaa
     4561 ggcggccatt agccaggcgt cagatgttgc cgcgttagat aatgtgcgcg tcgaatattt
     4621 gggtaaaaaa gggcacttaa cccttcagat gacgaccctg cgtgagctgc cgccagaaga
     4681 gcgtccggca gctggtgcgg ttatcaacga agcgaaagag caggttcagc aggcgctgaa
     4741 tgcgcgtaaa gcggaactgg aaagcgctgc actgaatgcg cgtctggcgg cggaaacgat
     4801 tgatgtctct ctgccaggtc gtcgcattga aaacggcggt ctgcatccgg ttacccgtac
     4861 catcgaccgt atcgaaagtt tcttcggtga gcttggcttt accgtggcaa ccgggccgga
     4921 aatcgaagac gattatcata acttcgatgc tctgaacatt cctggtcacc acccggcgcg
     4981 cgctgaccac gacactttct ggtttgacgc tacccgcctg ctgcgtaccc agacctctgg
     5041 cgtacagatc cgcaccatga aagcccagca gccaccgatt cgtatcatcg cgcctggccg
     5101 tgtttatcgt aacgactacg accagactca cacgccgatg ttccatcaga tggaaggtct
     5161 gattgttgat accaacatca gctttaccaa cctgaaaggc acgctgcacg acttcctgcg
     5221 taacttcttt gaggaagatt tgcagattcg cttccgtcct tcctacttcc cgtttaccga
     5281 gccttctgca gaagttgacg tcatgggtaa aaacggtaaa tggctggaag tgctgggctg
     5341 cgggatggtg catccgaacg tgctgcgtaa cgttggcatc gacccggaag tttactctgg
     5401 tttcgccttc gggatgggga tggagcgtct gactatgttg cgttacggcg tcaccgacct
     5461 gcgttcattc ttcgaaaacg atctgcgttt cctcaaacag tttaaataag gcaggaatag
     5521 attatgaaat tcagtgaact gtggttacgc gaatgggtga acccggcgat tgatagcgat
     5581 acgctggcga atcaaatcac tatggcgggc ctggaagttg acggtgtaga accggttgcc
     5641 ggtagcttcc acggcgtggt cgttggtgaa gtggttgagt gtgcgcagca tccgaacgct
     5701 gacaaactgc gtgtgacaaa agtaaatgtc ggcggcgatc gcctgctgga tatcgtctgc
     5761 ggtgcgccaa actgccgtca gggcctgcgt gtggcggtag ctaccattgg tgctgttctg
     5821 ccgggtgatt tcaaaattaa agcggcgaaa ctgcgtggcg aaccgtctga agggatgctg
     5881 tgctcctttt ctgagctggg aatttctgac gatcacagtg gcattatcga actgcctgcg
     5941 gatgcgccga ttggcaccga catccgcgaa tacctgaaac tcgatgataa caccatcgaa
     6001 atcagcgtga caccaaaccg tgccgactgc ttaggtatca ttggtgttgc gcgtgacgtt
     6061 gccgtgctga atcagctgcc gctggttgaa ccggaaatcg ttccggttgg tgcgaccatc
     6121 gacgacacgt tgccgattac agtcgaagcg ccggaagcct gcccgcgcta tcttggccgt
     6181 gtggtaaaag gcattaacgt taaagcgcca actccgctgt ggatgaaaga aaaactgcgt
     6241 cgttgcggga tccgttctat cgatgcagtt gttgacgtca ccaactatgt actgctcgaa
     6301 ctgggccagc cgatgcacgc tttcgataaa gatcgcattg aaggcggcat tgtggtgcgg
     6361 atggcaaaag agggcgaaac gctggtgctg cttgatggca ctgaagcgaa gctgaatgct
     6421 gacactctgg tcatcgccga ccacaacaag gcgctggcga tgggcggcat ctttggtggc
     6481 gaacactctg gcgtgaatga cgaaacgcaa aacgtcttgc tggaatgcgc tttcttcagc
     6541 ccgctgtcta tcaccggtcg tgctcgtcgt catggcctgc atactgatgc gtctcaccgt
     6601 tatgagcgtg gcgttgatcc ggcactgcag cacaaagcga tggaacgtgc gacccgtctg
     6661 ctgatcgaca tctgcggtgg tgaggctggc ccggtaattg atatcaccaa cgaagcaacg
     6721 ctgccgaagc gtgcaaccat cactctacgt cgtagcaaac tggatcgcct gataggccat
     6781 catattgctg atgagcaggt aactgacatt ctgcgtcgtc tcggctgcga agtgaccgaa
     6841 ggcaaagacg aatggcaggc agttgcgccg agctggcgtt tcgatatgga gattgaagaa
     6901 gatctggtcg aagaagtcgc gcgtgtttac ggctacaaca acatcccgga tgagccggta
     6961 cagacaagcc tgattatggg tactcaccgt gaagctgacc tgtcgctcaa gcgcgtgaaa
     7021 acgctgctca acgacaaagg ctatcaggaa gtgatcacct acagcttcgt tgatccgaaa
     7081 gtgcagcaga tgatccatcc aggcgttgaa gccttactgc tggcaagccc gatctctgtt
     7141 gaaatgtcag caatgcgtct ttctctgtgg accggcctgc tggcaaccgt ggtgtacaac
     7201 cagaaccgtc agcagaaccg tgtgcgcatt ttcgaaagcg gtctgcgttt cgtaccagat
     7261 actcaggcac cgttgggtat tcgtcaggat ctgatgttag ccggtgtgat ttgcggtaac
     7321 cgttacgaag agcactggaa cctggcaaaa gagaccgttg atttctatga tttgaaaggc
     7381 gaccttgaat ccgttctcga cctgaccggt aaactgaatg aggttgagtt ccgtgcagaa
     7441 gcgaatccgg cactgcatcc ggggcaatcc gcagcgattt atctgaaagg tgaacgtatt
     7501 ggttttgttg gagttgttca tcctgaactg gaacgtaaac tggatcttaa cggtcgcact
     7561 ctggtgttcg aactggagtg gaacaagctc gcagaccgcg tggtgcctca ggcgcgcgag
     7621 atttctcgct tcccggcgaa ccgtcgtgac atcgcggtgg tggtcgcaga aaacgttccc
     7681 gcagcggata ttttatccga atgtaagaaa gttggcgtaa atcaggtagt tggcgtaaac
     7741 ttatttgacg tgtaccgcgg taagggtgtt gcggaggggt ataagagcct cgccataagc
     7801 ctgatcctgc aagataccag ccgtacactc gaagaagagg agattgccgc taccgtcgcc
     7861 aaatgtgtag aggcattaaa agagcgattc caggcatcat tgagggattg aacctatggc
     7921 gcttacaaaa gctgaaatgt cagaatatct gtttgataag cttgggctta gcaagcggga
     7981 tgccaaagaa ctggttgaac tgtttttcga agagatccgt cgcgctctgg aaaacggcga
     8041 acaggtgaaa ctctctggtt ttggtaactt cgatctgcgt gataagaatc aacgcccggg
     8101 acgtaacccg aaaacgggcg aggatattcc cattacagca cggcgcgtgg tgaccttcag
     8161 acccgggcag aagttaaaaa gccgggtcga aaacgcttcg cccaaagacg agtaatctga
     8221 tctaactaaa aaggccgcta tgcggccttt tttcttttca ctgtcgaaga gtcaccgtaa
     8281 aatcaacgcc atgacacttc agcagaacgg ataccatgct gacacttgcc cgccaacaac
     8341 agcgacaaaa tattcgctgg ttattatgcc tgtcagtttt gatgctgctg gcgcttctct
     8401 taagcctttg cgccggtgaa caatggattt cgccaggtga ctggtttact cctcgtggcg
     8461 aactgttcgt ctggcaaatt cgcctgccac gtacgctggc tgtattgctg gttggtgcgg
     8521 cgctggctat atccggcgct gtaatgcagg cgttgtttga aaatcctctg gcagaacctg
     8581 gactacttgg cgtctctaac ggcgcaggcg tggggcttat cgccgcggta ttgcttgggc
     8641 aagggcaact ccccaactgg gcgctagggc tgtgtgcgat tgctggcgcg cttatcatca
     8701 ctttaatact cttacgtttc gcccgtcgtc atctttcgac cagtcggtta ttgctggctg
     8761 gcgttgcatt agggattatc tgcagcgcac taatgacgtg ggctatctac ttttccacct
     8821 ctgttgattt acgtcagctg atgtactgga tgatgggcgg ttttggcggc gtagactggc
     8881 ggcaaagctg gctgatgctg gcattgatcc ccgtgttgtt gtggatctgt tgtcagtcca
     8941 ggccgatgaa tatgttagca cttggcgaga tctcggcgcg gcaactgggt ttacccctgt
     9001 ggttctggcg caatgtgctg gtggcagcga ccggctggat ggttggcgtc agtgtggcgc
     9061 tggcgggtgc tatcggcttt attggtctgg tgatcccaca tattcttcgg ttgtgtggtt
     9121 taaccgatca tcgcgtatta cttcccggct gcgcgctggc aggggcgagc gcattgctgc
     9181 tggccgatat tgtagcgcgc ctggcattag ctgccgcaga gctgcctatt ggcgtggtca
     9241 ccgcaacgtt gggtgcgccg gtgtttatct ggttattgtt aaaagcagga cgttagccgc
     9301 aaaaagacgg tctatgatta aaagctaaga tttttactga ccacacccag gagaaacgat
     9361 gcaagattcc attctgacga ccgtagtgaa agatatcgac ggtgaagtga ccacgctgga
     9421 gaagtacgcc ggtaatgtgc tgttaattgt caatgtcgcc tcaaagtgtg gcttaacgcc
     9481 gcaatatgag cagttggaga atattcaaaa agcctgggcc gatcgaggtt ttgtggtgct
     9541 gggattcccg tgcaaccagt ttctggaaca agaaccgggc agcgatgaag agattaaaac
     9601 ttactgtacc accacatggg gggtgacgtt cccgatgttc agtaagattg aagttaatgg
     9661 cgaaggacgc catccgctgt atcaaaaatt gattgccgca gcgccgaccg cagtcgcgcc
     9721 ggaagagagc ggattctatg cccgtatggt cagcaaaggc cgtgcaccgc tgtacccgga
     9781 tgatatttta tggaattttg aaaaattcct ggttggcagg gacggaaaag tcatccagcg
     9841 tttttccccg gatatgacgc cggaagatcc cattgtgatg gaaagcatta aactggcgtt
     9901 ggcaaaataa tgtctattgt gatgcagtta caggatgttg cggaatctac ccgcctgggg
     9961 ccgctttcag gcgaggttcg ggctggggaa atcctgcacc tggtggggcc gaatggcgcg
    10021 ggtaagagta ccttactggc gcgaatggcc ggaatgacca gcggtaaggg aagcattcag
    10081 ttcgcggggc aaccactgga agcatggtcc gcaacaaaac tcgcgctgca tcgcgcctat
    10141 ctttcacaac agcagacgcc gccgtttgca atgccggtct ggcactacct gacactgcat
    10201 cagcacgata aaacgcgtac cgaactactg aatgatgtcg caggggcgct ggctcttgat
    10261 gacaaactcg gtcgtagcac caatcaactt tccggcggtg aatggcaacg cgtacgtctt
    10321 gctgcggtgg tgttgcaaat cacaccacaa gccaatcccg caggccaatt gctgcttctt
    10381 gatgagccga tgaacagtct tgatgttgcg caacaaagtg tgttagacaa aattctgagc
    10441 gcgctgtgtc agcaaggact ggcgattgtg atgagcagtc acgatctcaa ccacacgttg
    10501 cgtcatgcgc atcgggcgtg gttgctaaaa ggtggaaaaa tgctggccag tggacgcaga
    10561 gaagaggtgc tcacgccgcc aaatctggcg caggcctatg ggatgaattt tcgccgtctg
    10621 gatatcgaag gtcacagaat gctgatttcg accatctgat ttcattagta tcttgaaaat
    10681 cgggtaatta cgacgctaaa ttaatgcaag aataaaaaac agaggaaccg tacggaatgc
    10741 gtttctgcct tattttgatc acagcactgt ttctggccgg gtgtagccac cataaagcac
    10801 cgccgccaaa tgccagactt tctgattcga ttaccgttat tgccggtttg aacgaccagc
    10861 tacaaagctg gcatggcacg ccgtatcgtt atggtggcat gacgcggcgc ggtgtggact
    10921 gttcgggatt tgtggttgtg acgatgcgcg atcgtttcga tttgcatctg ccccgtgaaa
    10981 ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga cgagttgctg cctggtgacc
    11041 tggtcttttt caaaacgggt tccggacaaa atggtttgca tgtaggtatt tacgatacca
    11101 acaaccaatt tatccacgcc tctaccagca agggagtgat gcgttcctca cttgataatg
    11161 tctattggca gaaaaatttc tggcaggcga gacgaatcta gtatgtcgaa aaattgacgg
    11221 cgatgataca tcgccgtcaa tttactcctg aatacagtag tattttgcag acaaaaattc
    11281 cgtaaatgga ttattcgttt ataatcagga taatctaaaa aaaggccccc tgaaaaagtg
    11341 gttaacatgg aacaaatgaa agttactcct tctgtaattg ttatgtctta tggcctcatc
    11401 ccttttagcc ggatactgaa aaacatcctt cgagagggac ggttaccatg aagatttttt
    11461 tggagaatct ttatcattct gattgctatt tcctgccgat aagagataac cagcaactcc
    11521 ttgtcggcgt tgagttaatt acccatttct ccagtgaaga tggcacggtt cgtatcccga
    11581 cgagtcgggt tatagcgcaa cttaccgagg agcaacattg gcaattattt tcagagcagt
    11641 tggaattact aaaatcatgc caacactttt ttattcaaca taaactcttt gcctggctta
    11701 atttaacgcc acaagtggct acattattat tagacaggga taattacgca ggcgaactat
    11761 taaaatatcc gtttattgaa ctgttgatta atgagaatta cccgcatctc aatgaaggaa
    11821 aagataatcg cgatcttttg tccttatcgc aaatgtatcc ccttgtgtta ggcaatttgg
    11881 gagcaggtaa tagcacaatg aaagcggttt ttgatggtct tttcacccgc gtaatgctgg
    11941 agaaaggttt tattcagcaa caaatcacgc atcgctcctt cgaaccgttt attcgtgcga
    12001 tccaggcgca aatttcccct tgttgcaatt gcatcatcgc ggggggcatt gatacgccag
    12061 aaatattggc acagattacc cccttcgatt tccatgctct ccagggatgc ctgtggcctg
    12121 ccgttcctat aaatcagata acgacgttgg ttcagcgata accctcctgt ttgctggtgt
    12181 ttaagacgag agtaaccgtc tacactatca aacagatagg atctatgacc ctgtctttta
    12241 ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc actttcccct acgcctttaa
    12301 ataatgcccg gctgatttgg cataataccg aactcgctaa cacgctgagt attccatcgt
    12361 cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac cctactgcct ggtatgtcac
    12421 cactggcaca ggtttacagt ggtcatcagt tcggcgtctg ggcgggccaa ctgggtgatg
    12481 ggcgcggcat tttacttggc gaacaactgc ttgctgatgg cactacaatg gactggcatc
    12541 tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga tggacgggcg gttttacgtt
    12601 cgacgatacg agaaagtctc gccagtgagg cgatgcatta tctgggcatt ccgacgaccc
    12661 gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg ggaaacggtg gagccaggcg
    12721 cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg tcatttcgaa catttttact
    12781 atcgccgcga gccggaaaag gttcgtcagt tggctgactt tgccattcgt cattactggt
    12841 cacatcttga agacgatgag gacaaatacc gtctctggtt taacgatgtt gtcgcacgta
    12901 ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc acatggggtg atgaataccg
    12961 acaacatgtc gctgctggga ctgacgcttg attacgggcc gtttggtttt cttgatgatt
    13021 acgaacccgg ttttatttgt aatcactcgg atcatcaagg gcgttatagc tttgataatc
    13081 aacctgctgt cgcgttgtgg aatttacagc gtctggcgca gacattgtca ccatttgttg
    13141 ccgtagatgc cctgaatgag gccctggaca gctatcagca ggttttgttg acgcattatg
    13201 gacaacggat gcggcagaaa ctgggcttca tgacggagca aaaagaggat aacgcgctac
    13261 tgaatgaatt attcagtctg atggcgcgag agcgcagcga ttatacccgc acattccgca
    13321 tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct acgtgatgag tttattgatc
    13381 gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg tttgcaacaa gacgaggtta
    13441 gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc agctctggta ttgcgcaact
    13501 ggttggcgca acgggcgatt gaagcggcag aaaagggtga tatgatggaa ttgcaccgcc
    13561 tgcatgaggc gttgcgaaat cctttcagcg acagagatga tgactatgtc agtcgtccac
    13621 ctgactgggg taaacggctg gaagtcagct gctcgagtta aaggctactt agttaacagc
    13681 agcttgccag cctgagtttt acggagcaga tattcttgcc cgtcatgatc gataatcagt
    13741 ttgccatccg gacctaacag tgtctggctg gtaattcgcc ggatgggctg cggggaagcg
    13801 gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc tatccgtata acgcataaaa
    13861 attaataata attgatataa caatgattct tattatcaat attatggcag gccagagcaa
    13921 gcagtaatgt gttgagaaga caacaagttg cgcagaaatg cccatcccat caggaatggg
    13981 cacgcattca gaagcgggta tctaccgcag aggcgagttt ttcgaccagg cgttcggtat
    14041 cttcccagcc cagacacggg tcggtaatgg attgaccgta agtgagcggc tgaccgccga
    14101 cgattttttg cgttccttcg cgcaggaaac tttccgccat aattccagca atctccgtag
    14161 agccattgcg gatttgctga caaatatcct cacaaacttc taactggcga cggtgctgct
    14221 tctggcagtt accgtggctg aaatccacca ccagatgttc aggtaaatca aactcgtgca
    14281 gcgtatcgca ggctgcggcg atatcatcgg catgataatt cggttttttg ccgccacgca
    14341 taataatgtg gccatacggg ttgccgctgg tctgatagat ggtcatctga ccatttttgt
    14401 ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat agcatccacg gcaatccgcg
    14461 tgttgccatc ggtaccattt ttaaaaccta ccggacagga gagtgctgaa gccatttcgc
    14521 ggtggatctg actttcggta gtacgagcgc caatcgcgcc ccaactgatt aaatcagcaa
    14581 taaactgacc ggtcaccata tcgaggaact cagtcgcggt agggacaccc agctcattta
    14641 cctgtaaaag taatttgcgc gccagctcca gaccgtgatt tacccgatag ctgccgttta
    14701 aatccggatc ggagattagc cctttccagc cgacaacagt tcgtggtttt tcaaaatagg
    14761 tgcgcattac gatttccagc cgtgactggt actggttgcg cagcgactgc agacgggtgg
    14821 cgtactccat tgcagcggtg agatcgtgga tcgagcaggg gccaataatg accaacagtc
    14881 gcttatcttc accattcagt attttttcaa ttctgcggcg ggagtcggtg acatgggtgg
    14941 cgacgccagg cgttacggga taccgtagcg cgagttcggc gggcgttacc aggctctcaa
    15001 tacgcgcagt acggagttcg tcagttctgt tcattacaag tctcagaaat ttgttgcttc
    15061 tacggcagga ctaccggaaa gtgatgcgca tcacgataaa ccaatcgcag ctgatttcaa
    15121 gtgcgaggtg tgtcacacct cgccgggagg ttagcatgat aacaaaaaaa caaaccaatg
    15181 cactagttct ctagtacatt cggcgactaa ggcccatgat atcgaggatc ttggtggcaa
    15241 tctcttctac cgaataattg gtactgttaa tccacgggat ctgattttta cggtacaagg
    15301 cttccacttc cgcgacttcc atcctgcact gacgaagcga ggcatagcga ctgttctccc
    15361 gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat agtcaggccg aacaatttat
    15421 gctgaagcgg tttgagcgac gcgggtagca ccagattatc catatcgtcg gcaataaagg
    15481 ggtagtttgc cgcgcggata ccaaattgca ttgccagata cagactggtg ggggttttac
    15541 cacagcgaga aacaccgagc aggatcacct gagcctgatc cagattgcgc aacgaaatgc
    15601 cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg cgcatcatat ttattgagat
    15661 tattagggtt aaggccatgg gtacgatgag caatcggcgt tggatccagt ttcatctctt
    15721 gttgtagcgg ggcaaccagc gcctgaacga tatcctggca aaagccttca ctttgcaaga
    15781 tgatggcgcg aatctccggc aacacgatgg agtagaagac cagcgggcgc acgcctgtct
    15841 ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc acggctctca ttttcgacaa
    15901 acggcagcgt gatgctgctg atagtgacgg gaaattgtga cattactgcg tgtcctaata
    15961 cctccgcagt tattgccgta ccatcagaaa tataaaaaac gtggcgatca acagcattat
    16021 ccattttgtt cttcccgtga tgcagacata attctatgaa agcataaatt aaaaacgcac
    16081 agaagcgtag gacgtaatgt ctggtttata aaatgaacct tcaattttat tttttatgaa
    16141 aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa attgtcgaat
    16201 agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat atttgcttga
    16261 acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca gaaatgtgtt
    16321 tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg ctcgtcaccg
    16381 ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg gggcaaaaat
    16441 gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt tccgaatggt
    16501 ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt aaaccagcgc
    16561 atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc gaaagcgggc
    16621 gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga aaacgccata
    16681 cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc ggtacgctcc
    16741 tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga aaccttcctc
    16801 aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc ttctctgttt
    16861 aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg cgtggcgctc
    16921 tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt gatgttctcc
    16981 attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg gggccttggg
    17041 gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa accgacactg
    17101 gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat ccgcatggtt
    17161 tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc gcaggaacag
    17221 cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca ggccgtacaa
    17281 attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg ccacaccggc
    17341 aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca ggtcatggag
    17401 cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat cggtcatcgc
    17461 atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg catcgaacct
    17521 ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat gaagaaagca
    17581 tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat cgctcgtgaa
    17641 ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa agacggtgag
    17701 aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga gttgctggaa
    17761 tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa agtgatgatg
    17821 aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga aggcgtgggc
    17881 cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg cgcactgctt
    17941 gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat gaaaggtttt
    18001 gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac gctgggtgcc
    18061 gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa cgaatatgcc
    18121 aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct cggcttccgt
    18181 ggcgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga gtgtgaagca
    18241 gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat cccgttcgtg
    18301 cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg gctgaaacgt
    18361 ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc cttgctggcc
    18421 gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat gacgcagctg
    18481 gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga gcgcaacgat
    18541 gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg caaatatgtc
    18601 gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt gatggaagag
    18661 gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt aagcctggct
    18721 gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta tgtgatcccc
    18781 gaagatgaaa cttattcaat ctcttcacag atatcctgcg ttaatcgccg tataatatct
    18841 tttcttaaca aaaacttttg tattttacct gaggcagtgc gcggtagttt ttcgattacc
    18901 acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa agccaccatc
    18961 tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca tgatcgttca
    19021 cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg atgctgcaat
    19081 aaaatatctt ccacttcacg gctgctaata tattcgccgc cgcggacaat aatatctttt
    19141 ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc gccgctgtaa
    19201 taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa ataccccata
    19261 aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa ggtcttgcgt
    19321 gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt atgcataaag
    19381 cgcgacaaag aatcatcgag attcaccacc gcatgcggcg aactttctgt ggaaccataa
    19441 acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt ggggattgtg
    19501 gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt ctctagtaaa
    19561 ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg ctgctgctca
    19621 agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg agcgccaatt
    19681 aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg cgcaggcatc
    19741 ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc actggcgaga
    19801 atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga ggtaaacagc
    19861 accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat attgtcggcg
    19921 ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac gccgacaatt
    19981 tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg acgcgtttgt
    20041 ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac ccacaccagt
    20101 tctgcttccc gctaggaagg caacagcggc acggaaaccg caccgatttt caggcaggca
    20161 agatggataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg atcgcctgat
    20221 tcaatgccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc gagcgcgcta
    20281 taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat cgcacgagcg
    20341 gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg acgatacgcc
    20401 gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg aaacagaaca
    20461 tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa tcaataccga
    20521 aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc acccgacagg
    20581 tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca tcctgcttat
    20641 aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg ggattttccg
    20701 tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct aatttgatgt
    20761 cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc catctttcag
    20821 caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta ccggctggtt
    20881 tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc gtgggtattg
    20941 gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct gcatatcacg
    21001 catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg cggagaaatc
    21061 cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg caatggctaa
    21121 atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt caccaacgat
    21181 catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg gcaccatatg
    21241 cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg ggtgttgttt
    21301 aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac ccaatccaca
    21361 aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc ccatcaggcc
    21421 gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa tattaaagcg
    21481 atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag cgtaatgatg
    21541 cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa cgccatcagc
    21601 cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag tgaccttgtt
    21661 tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc ccgcctgctc
    21721 ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag atgcgtgttg
    21781 cggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt cggttaagaa
    21841 ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa agcctggaat
    21901 gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac atccggcact
    21961 gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca gtgcggcaac
    22021 gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg acatgcgtta
    22081 tgctcccttc ccgctgcgtt tacagcggga gatgtggttg aaaggggaat tagcggctca
    22141 actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg tagtcggcat
    22201 agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt acgccgttgc
    22261 cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga tcggatttca
    22321 gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg ccttcggcaa
    22381 ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg accattttca
    22441 ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct ttgctcaggt
    22501 ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga cgcggagcaa
    22561 cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac ggttcctgaa
    22621 caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga gcgaacgcta
    22681 acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc accgccgtgg
    22741 catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga gctttaccac
    22801 gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg gcagcaatgc
    22861 tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca taaatacatt
    22921 ttggatcata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg gcatacacct
    22981 gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga ttatcgctaa
    23041 agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct
    23101 ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc gagaatgatg
    23161 tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag tggcgcgctc
    23221 tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc cgcgccgaga
    23281 atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca gagcacggct
    23341 ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat caccagtgta
    23401 ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag aatttctccg
    23461 accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa
    23521 tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag aggcagtgca
    23581 tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga tagcacgtct
    23641 ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc tatctcatcg
    23701 tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg gctgattttg
    23761 gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc ctgttcttca
    23821 ggcaccagct taaagcaggt tattattttc atggtttctc ctgtgagtcg catacggaac
    23881 catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt gcggggtttt
    23941 cgacggagca agctgttttc cttgccaaca atcttgcaaa aaaaatgcga tggctaaaac
    24001 aaaagtgcaa tttgcggcaa tatagttatt ccatgaccgt acaatcttgc attcagtgta
    24061 aataatcttg tttagctctc gctaaacgcg gttgtataac cgcaatacac gccgtatttt
    24121 atccggcata tattgttttt gcttgctcat ggaataggga ggttgtcatg tatcaacgct
    24181 gttttgataa tgccagtgaa acgctgtttg tcgccggtaa aacgccacgg ctttcacgtt
    24241 ttgcatttag cgatgatcca aaatgggagt ctggacatca cgttcatgac aatgaaaccg
    24301 agctgattta cgtcaagaaa ggggttgcaa ggtttaccat cgattcttcg ttatatgtcg
    24361 cacatgcgga tgacattgtg gtgatagaac gcggcaggct gcatgcggtg gcctctgacg
    24421 ttaacgatcc ggcaacgacg tgtacctgtg cgctgtacgg ctttcagttt cagggggctg
    24481 aggaaaatca gttactgcaa ccgcattctt gtccggtaat tgccgcgggg cagggaaaag
    24541 aagtcataaa aaccttattt aatgagctaa gtgtgatttt gccgcaaagt aaaaatagcc
    24601 aaacatcttc gttatgggat gcatttgcct atacattagc aattctttac tacgaaaact
    24661 ttaaaaatgc ttatcgttcg gagcaggggt atattaaaaa agatgttctg ataaaagata
    24721 ttcttttcta tctgaataat aattatcgcg aaaaaatcac tttagaacag ttatcgaaaa
    24781 aatttcgtgc cagcgtcagt tatatttgcc atgaatttac caaagagtat cgtatttccc
    24841 ctattaacta tgttattcaa cggcgtatga cggaagcaaa atggtcactc attaatactg
    24901 aattatcaca ggcagagatc tcctggcgtg tgggttatga aaatgtcgat cactttgcca
    24961 aactgttttt gcgccatgtc ggttgttcgc ccagcgatta ccgtaggcaa tttaaaaatt
    25021 gttttgcgga acaagaaatc ttatctgaat ttcctcaacc ggtaagtctt gccggataaa
    25081 tctggcggcg cactttgtta aaaagctgcg ccgcctgcag attatttgtt ctgatagtct
    25141 ttcaggatct gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc gccgatacgt
    25201 tcacaacgga catcacgcca gaagcgggag acgcgcgcct catcggtata gcccagaccg
    25261 cccatgattt gaatcgcatc atcaatgact tccattgcgg tacgtgcaca gtacagtttt
    25321 gccagcgccg cgctggtgcg cagtgattga tgctgatcgg cttgccatgc cactttcagc
    25381 accatgttgc gcatgttgtc gatcttaatc gccatcagcg ccagtttttc ctggatcatc
    25441 tggttatgcc caatgggttt accaaaagcg atacgctggt tggcatagcg agcggcatct
    25501 tcaaaggcac actcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca
    25561 aagttgtaca tcacattgag gaaacccatt ccttcttcgc ccaccatgtc gctctcttca
    25621 acttccacgt tgtcgagata gacttcgcag gtgctgagca tatgccagcc gattttatgc
    25681 agcgggttaa tcttaatgcc gggcttactg gagtcgaccc accacagggt gaaggctttt
    25741 ttgggatctt tcggttgcgg atcgcgcgcc aacaccagca tatacgggta ctctttcgca
    25801 ccggtaataa aggttttctg tccgttgatg taaaccttgc cgtttttacg cgtgtaagtg
    25861 gtagtggcac tgttgttgtc tgagcctgcg cctggctccg tcaacgccag ggcataggcg
    25921 ggatcacccg tttccagtgt gctttctgcc gttttacgta gctgctctgc agaaccgaaa
    25981 cggcgcatac tgtgaataca ttgaccgttg gtaatcaaaa atgccggagc accgcatttt
    26041 gacacttcca tcagcgccag catctgggta acgtaatccg cagggatacc gccgaactct
    26101 tccggcacac caagcatgga aataccgtta tccgccagtg cccgcataaa ctcacgcggg
    26161 tatgttccgt tttgatcgca ggtgcgggaa tactcttccg gaaagttagt cgtaatcaac
    26221 tcgcgaatac tggccagcag cagttcttgt tcttcagtta aagaaaaatc tatcgtgata
    26281 ctcctgttaa tgagccgctt caggcaggac aaaacccatc gccgcatcga taaataatct
    26341 ttcgtccatc agtttgagtt ctggcgaaat cactggggtg aaatccattt tgtcgagaat
    26401 atctttttgt aaatcgacgc caggagcgat ttcgattaaa tgcaggccgt cttctttcag
    26461 cgtgaataca gcacgttcgg tgatgtaacg aacatccagt ccccgttcga gcgcgatttt
    26521 tccgctgaaa gtaatttccg gtagttcccg aataaatttc ttcacccgtc cttcctggac
    26581 gatatttaat ttgccgtcgg taatttctgt ttttaaactg cccg
//
LOCUS       DADLSB010000040        26364 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.040, whole genome shotgun sequence.
ACCESSION   DADLSB010000040 DADLSB010000000
VERSION     DADLSB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.040"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..427
                     /locus_tag="JN331_001672"
     CDS             <1..427
                     /locus_tag="JN331_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7259843.1"
                     /translation="VNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(459..1295)
                     /locus_tag="JN331_001673"
     CDS             complement(459..1295)
                     /locus_tag="JN331_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase YeiG"
                     /protein_id="HAY7259844.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            1553..2221
                     /gene="folE"
                     /locus_tag="JN331_001674"
     CDS             1553..2221
                     /gene="folE"
                     /locus_tag="JN331_001674"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY7259845.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            2238..3395
                     /locus_tag="JN331_001675"
     CDS             2238..3395
                     /locus_tag="JN331_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY7259846.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            3537..4577
                     /gene="galS"
                     /locus_tag="JN331_001676"
     CDS             3537..4577
                     /gene="galS"
                     /locus_tag="JN331_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY7259847.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            4857..5855
                     /gene="mglB"
                     /locus_tag="JN331_001677"
                     /pseudo
     CDS             4857..5855
                     /gene="mglB"
                     /locus_tag="JN331_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
     gene            5916..7436
                     /gene="mglA"
                     /locus_tag="JN331_001678"
     CDS             5916..7436
                     /gene="mglA"
                     /locus_tag="JN331_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY7259848.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            7452..8462
                     /gene="mglC"
                     /locus_tag="JN331_001679"
     CDS             7452..8462
                     /gene="mglC"
                     /locus_tag="JN331_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY7259849.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8533..9768)
                     /gene="preA"
                     /locus_tag="JN331_001680"
     CDS             complement(8533..9768)
                     /gene="preA"
                     /locus_tag="JN331_001680"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY7259850.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(9762..11000)
                     /locus_tag="JN331_001681"
     CDS             complement(9762..11000)
                     /locus_tag="JN331_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7259851.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(11194..11433)
                     /locus_tag="JN331_001682"
     CDS             complement(11194..11433)
                     /locus_tag="JN331_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY7259852.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(11436..12155)
                     /gene="sanA"
                     /locus_tag="JN331_001683"
     CDS             complement(11436..12155)
                     /gene="sanA"
                     /locus_tag="JN331_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY7259853.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(12305..13189)
                     /gene="cdd"
                     /locus_tag="JN331_001684"
     CDS             complement(12305..13189)
                     /gene="cdd"
                     /locus_tag="JN331_001684"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY7259854.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(13319..14014)
                     /locus_tag="JN331_001685"
     CDS             complement(13319..14014)
                     /locus_tag="JN331_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY7259855.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(14011..14409)
                     /locus_tag="JN331_001686"
     CDS             complement(14011..14409)
                     /locus_tag="JN331_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY7259856.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYILIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            14648..15595
                     /gene="dusC"
                     /locus_tag="JN331_001687"
                     /pseudo
     CDS             14648..15595
                     /gene="dusC"
                     /locus_tag="JN331_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
     gene            complement(15966..16049)
                     /gene="yohP"
                     /locus_tag="JN331_001688"
     CDS             complement(15966..16049)
                     /gene="yohP"
                     /locus_tag="JN331_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY7259857.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            16183..17709
                     /gene="mdtQ"
                     /locus_tag="JN331_001689"
     CDS             16183..17709
                     /gene="mdtQ"
                     /locus_tag="JN331_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY7259858.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDGVETDINASKTRQQ
                     LNDVAGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR
                     "
     gene            17762..18523
                     /locus_tag="JN331_001690"
                     /pseudo
     CDS             17762..18523
                     /locus_tag="JN331_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(18640..19218)
                     /locus_tag="JN331_001691"
     CDS             complement(18640..19218)
                     /locus_tag="JN331_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7259859.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            19388..19975
                     /locus_tag="JN331_001692"
     CDS             19388..19975
                     /locus_tag="JN331_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY7259860.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVQEVLLALTVILWGYGYRLF"
     gene            20149..21081
                     /gene="pbpG"
                     /locus_tag="JN331_001693"
     CDS             20149..21081
                     /gene="pbpG"
                     /locus_tag="JN331_001693"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY7259861.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(21119..22834)
                     /gene="dld"
                     /locus_tag="JN331_001694"
     CDS             complement(21119..22834)
                     /gene="dld"
                     /locus_tag="JN331_001694"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7259862.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            23030..25327
                     /gene="bglX"
                     /locus_tag="JN331_001695"
     CDS             23030..25327
                     /gene="bglX"
                     /locus_tag="JN331_001695"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY7259863.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            25579..>26364
                     /gene="osmF"
                     /locus_tag="JN331_001696"
     CDS             25579..>26364
                     /gene="osmF"
                     /locus_tag="JN331_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY7259864.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQ"
BASE COUNT         6509 a         6589 c         6948 g         6318 t
ORIGIN      
        1 cgttaacaaa gctcgtattc agggcgtgga aaccgaactg aaaattccgt tcaacgatga
       61 atggaaactg tcgatcaact acacctacaa cgatggtcgt gatgtcagca acggcgaaaa
      121 caaaccgcta tccgatctgc cgttccatac tgctaacggt acgctggact ggaaaccgct
      181 ggcgctggaa gactggtcat tctatgtttc tggtcactat accgggcaga aacgcgccga
      241 cagcgcgacg gctaaaacac cgggcggtta taccatctgg aataccggcg cggcctggca
      301 ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag
      361 tcgtgacgac tacagctata acgaagacgg acgtcgttac tttatggcag tggattatcg
      421 cttctgatga gaagatgccc ggcgaaccgg gcggactttc acttcagtaa atactgtgca
      481 tggaagcgca ggtgatcctc tataaaagag gcgatgaagt agtaactgtg atcatatccc
      541 ggctgaatac gcagcgtcat cggccacgct ttctggcgcg cggcttctgc caataccgca
      601 ggttgcaact ggtcggcaag aaactgatca ttatcgccct gatcgataag cgtcgggatc
      661 gcatcctgcg cgttactggc atacatcagc gcgcaactat cccattccag ccatgcattt
      721 ttgtcctcac ctaaataggt gctaaacgct ttgattcccc acgggacgct gcacggattc
      781 acaatgggcg caaaggccga aacgctggtg tatttacccg gatttttcag cgccataatc
      841 agcgcaccgt gaccacccat tgagtgaccg ctaatggcgc agcggtcgct gacattaaat
      901 tgcgactgaa ccagcgccgg taattcatcg cgcagataat catacatccg gtaatgcgtc
      961 gcccacggcg gttgcgtggc attaagataa aagcctgcgc cctggcctaa atcgtagcca
     1021 tcgtcgttgg caaccttttc gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc
     1081 agttccgccg ctacccgctg ggcaccagcc ttggtggtga agttctcgtc attgcaggtt
     1141 aatccggaaa gccagtacag cactggtggc ggggtgtgat cacgaggggg tgggagaaag
     1201 atactgaacg tcatcgggca gtttaaggta ctggagtcgt gtctccagcg ttgctgccag
     1261 ccttcaaaac agcggtgctc ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg
     1321 caatttcttc atcataccgt ttattcaatg tgctgtgagt aactttcact tccgtatttg
     1381 cataacgatg ttttaacatc tgctgatgaa aggcagcggc aattacaata attatcgctg
     1441 tgaatactgg attatgtgcg ccgcctcacg cacaataatc aagctgtaaa tcagcttaat
     1501 aactttgccc ccacgcaggg cggaggcgtc acacctgcag gagaaatcat aaatgccatc
     1561 actcagtaaa gaagcggccc tggttcatga agcgttagtt gcgagaggac tggaaacacc
     1621 gctgcgcccg cccgtgcatg aaatggataa cgaaacgcgc aaaagcctta ttgctggtca
     1681 tatgaccgaa atcatgcagc tgctgaatct cgacctggct gatgacagtt tgatggaaac
     1741 gccgcatcgc atcgctaaaa tgtatgtcga tgaaattttc tccggtctgg attacgccaa
     1801 tttcccgaaa atcaccctca ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg
     1861 cgatatcact ctgaccagta cctgtgaaca ccattttgtt accatcgatg gcaaagcgac
     1921 ggtggcttat atcccgaaag attcggtgat cggtctgtca aaaattaacc gtatcgtgca
     1981 gttctttgcc cagcgtccgc aggtgcagga acgtctgacg cagcaaattc ttattgcgct
     2041 acaaacgctg ctgggcacca ataacgtggc tgtctcgatc gacgcggtgc attactgcgt
     2101 gaaggcgcgt ggcatccgcg atgcaaccag tgccacgaca acgacctctc ttggtggatt
     2161 gttcaaatcc agtcagaata cgcgccacga gtttctgcgc gctgtgcgcc atcacaactg
     2221 attaaaaggc aggaaccatg gagcgcaacg tcacgctcga ttttgttcgc ggcgtcgcca
     2281 ttctggggat cctgctatta aacatcagcg cctttgggct accaaaggcg gcttatctca
     2341 atcccgcctg gtacgatgct attacgccgc aggatgcatg gacctgggca tttctcgatc
     2401 tcatcgggca ggtgaaattc ctcacgcttt ttgcgctgct gtttggtgct ggcctgcaaa
     2461 tgttgctgcc ccgtggcaga cgctggatcc agtcgcggtt aacgctgtta gtcttgttgg
     2521 gctttattca cggtttattg ttctgggacg gcgatattct gctggcttac gggctggtgg
     2581 gcttaatctg ctggcggctg gtgcgcgatg cgccatcggt aaaaagcctg tttaataccg
     2641 gcgtcatgct ttatctggtg gggcttggcg ttttgctgtt attggggctg atttccgaca
     2701 gccagaccag ccgcgcctgg acgccggatg catcggctat tttgtatgaa aaatactgga
     2761 agcttcacgg cggcgttgaa gcgatcagta atcgtgccga tggtgttggc aacagtttac
     2821 tggcactggg cgcacagtat ggctggcaac tggcagggat gatgctcatt ggtgcggcgc
     2881 tgatgcgcag cggctggctg aaagggcagt tcagcttacg tcactatcgt cgtactggtt
     2941 ttgtgctggt ggcgattggg gtgaccatta accttcctgc catcgccctg caatggcagc
     3001 tggactgggc gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc
     3061 cgtttcaggc gattggctat gcgtcgctgt tttatggctt ctggccgcaa ttgagccgct
     3121 ttaagctggt gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac tatctattgc
     3181 aaacgctgat ttgtaccacg cttttttacc acctcggttt gtttatgcat tttgaccgcc
     3241 tggagctgct ggcgtttgtt attccggtat ggctggcgaa tatcctcttc tctgttatct
     3301 ggctgcgtta cttccgccag gggccggtgg aatggctctg gcgtcagtta actttgcgtg
     3361 ctgccggacc ggcaatatct aaaacatcaa gataacgatc tggatcacat tcgttaacaa
     3421 aacggctgta accgtttcca ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg
     3481 acaaaatagc cgccagcaag cagtcattta ctgcaatctc ataacaggta gtgaatatga
     3541 tcaccattcg tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt tcccgggtgc
     3601 tcaataacag cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg
     3661 agctggatta tcggccaaac gccaatgccc aggcgctggc aactcaggtt agcgacacca
     3721 ttggcgtggt ggtgatggac gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg
     3781 atctggtcgc ccagcagcat cagaaatacg tgctaatcgg caatagctat catgaagcgg
     3841 aaaaagagcg tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc
     3901 actcaaaagc attgagtgac gatgaactgg cacaatttat ggataacatt cccggtatgg
     3961 tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg gataatctca
     4021 gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt attggttatc
     4081 tttcttccag ccacggcatt gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt
     4141 tgaaagagca ggatattatt ccgccggaaa gctggattgg cactggtacg ccggacatgc
     4201 cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa tctacaactt accgctgtat
     4261 ttgcttataa cgacaatatg gccgctggcg cactgacagc attaaaagat aatggcattg
     4321 cgattccgtt acatctctca atcatcggtt tcgatgatat tcccatcgcc cgttacaccg
     4381 acccgcaatt aacgaccgtg cgttatccca ttgcttcaat ggctaaatta gccaccgaac
     4441 tggccttgca gggggcagca ggcaatattg atcctcgtgc cagccactgt tttatgccga
     4501 cgttagtgcg tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc actaactcaa
     4561 caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc ctaacaatgt
     4621 gatagctatg attgcaccgt tttaacgttg taacccgtat gtaacagtga ataatcactt
     4681 tcaccgaggt aatagcgtca taacaataat taaagccgtt ttctggagcg ttaccgggca
     4741 tggaagaacg aattttaaaa agtgagcttc ggcgttcagt aacacttcat taactctact
     4801 gccccgccga gcatttatct caagcactac cctgcataag aaaaaccgga gataccatga
     4861 ataagaaggt gttaaccctg tctgctgtga tggccagcat gttattcggt gccgctgcac
     4921 acgctgctga tactcgcatt ggtgtaacaa tctataagta cgacgataac tttatgtctg
     4981 tagtgcgcaa ggctattgag caagatgcga aagccgcgcc agatgttcag ctgctgatga
     5041 atgattctca gaatgaccag tccaagcaga acgatcagat cgacgtattg ctggcgaaag
     5101 gggtgaaggc actggcaatc aacctggttg acccggcagc tgcgggtacg gtgattgaga
     5161 aagcgcgtgg gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc
     5221 tggatagcta cgacaaagcc tactacgttg gcactgactc caaagagtcc ggcattattc
     5281 agggcgattt gattgctaaa cactgggcgg cgaatcaggg ttgggatctg aacaaagacg
     5341 gtcagattca gttcgtactg ctgaaaggtg aaccgggcca tccggatgca gaagcacgta
     5401 ccacttacgt gattaaagaa ttgaacgata aaggcatcaa aactgaacag ttacagttag
     5461 ataccgcaat gtaggacacc gctcaggcga aagataagat ggacgcctgg ctgtctggcc
     5521 cgaacgccaa caaaatcgaa gtggttatcg ccaacaacga tgcgatggca atgggcgcgg
     5581 tagaagcact gaaagcacac aacaagtcca gcattccggt gtttggcgtc gatgcgctgc
     5641 cagaagcgct ggcgctggtg aaatccggtg cactggcggg cacagtactg aacgatgcta
     5701 acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc aaaggtgcgg
     5761 ctgatggcac caactggaaa atcgacaaca aagtggtccg cgtaccttat gttggcgtag
     5821 ataaagacaa cttggctgaa ttcagcaaga aataagactg attgggcgca gtcgtttact
     5881 gcgcccttat tgtatggcca acaaggtaaa attatatggt cagctcaacg actccgtctt
     5941 ccggggaata cttgttggaa atgagcggta tcaacaagtc ctttcctggt gttaaggcac
     6001 ttgataacgt taatttaaaa gtccggccac attctatcca tgcattaatg ggggaaaacg
     6061 gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca
     6121 ccattttatt ccagggtaaa gagatcgatt tccattccgc caaagaggca ctggaaaatg
     6181 gtatttcgat ggtacaccag gagttaaacc tggtattaca acgttcggtg atggataaca
     6241 tgtggctggg gcgatatccc accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg
     6301 aaaccaaagc gatttttgat gaactggata ttgatatcga tccgcgtgcg cgcgtcggca
     6361 cattatccgt ttcgcaaatg cagatgattg aaatcgccaa agcgttttcc tataacgcga
     6421 aaattgtgat tatggatgaa ccgacttctt cgttaaccga aaaagaggtc aatcatctgt
     6481 tcactattat tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa
     6541 tggaagaaat cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg
     6601 ccaccgaacc gctggcagga ctgacgatgg acaagatcat cgccatgatg gttgggcgtt
     6661 ctcttaatca gcgtttccct gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc
     6721 gtaacctaac gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag
     6781 gggagatcct cggtattgcc gggctggtgg gggcgaaacg taccgatatt gttgagacgt
     6841 tatttggtat tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa cagatcaata
     6901 accataatgc caacgaagcc ataaaccacg gatttgcact ggtaactgag gagcgccgct
     6961 caacgggaat ttatgcctat ctggatattg gttttaactc gttaatttcc aatattcgca
     7021 actacaaaaa taaagttggt ttactggata actcgcggat gaaaagcgat acccagtggg
     7081 tgattgattc gatgcgggta aaaacgccgg gtcatcggac gcaaattggt tcgctctccg
     7141 gtggtaatca gcaaaaggtg attattggtc gctggctact aacgcaacca gaaatattaa
     7201 tgctcgatga accgacgcgc ggtattgatg tcggggcgaa gtttgaaatt tatcagttaa
     7261 ttgccgaact ggcgaagaaa ggcaagggga ttattattat ctcctctgaa atgcctgagt
     7321 tgttagggat aacagaccgt attctggtca tgagcaatgg tctcgtttcc ggaattgtcg
     7381 atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt
     7441 aggggcttcc catgagtgcg ttaaataaga aaagttttct tacttacctg aaagagggcg
     7501 gtatttatgt cgttctttta gttttgctgg cgattattat tttccaggac ccaacatttt
     7561 taagtctgtt gaacttaagt aatattctca cccagtcatc ggtgcgtatt attatcgcgc
     7621 tcggtgtggc agggttaatt gtcacccagg ggaccgatct ttctgctggt cgtcaggtag
     7681 ggctggcggc agtggtggct gcgacattat tgcagtccat ggataacgcc aacaaagtgt
     7741 tcccggaaat ggcgacgatg ccgattgcgc tggttattct gattgtctgt gccattggtg
     7801 cggtgatcgg tttgatcaac ggtctgatta tcgcttatct caacgtgacg ccgttcatta
     7861 ccacgctcgg cacgatgatc atcgtctatg gcatcaactc gctctattac gactttgtcg
     7921 gggcgtcgcc aatttctggt tttgacagtg gcttctctac ctttgctcag ggctttgtcg
     7981 cgctggggag tttccgtctc tcttacatca ccttctacgc gttgattgcg gtggcgttcg
     8041 tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat ttttgccatt ggcggtaacc
     8101 cggaagcggc aaaagtatct ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt
     8161 tgtctggcgt gttctatgcc tttggcggga tgttagaagc cggacgtatc ggctctgcca
     8221 ccaacaacct cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggtg
     8281 tatcgttcag cggcggtgtg gggacggtga ttggcgtggt gaccggggta attattttta
     8341 ccgtcatcaa ctatggcctg acgtatatcg gcgtaaaccc atactggcag tacatcatca
     8401 aaggggcgat tattatcttc gccgtagcgc tggattcact gaaatacgcg cgtaagaagt
     8461 gatgtgattg cggatattgc cggatgcgct gcatccggca atcggtgcca aatgccggat
     8521 gcgtctctgg ttttacaacg ttaccgggtg ttctttctcg cctttcttaa acttcacttc
     8581 cccgagctca atacaaccca ccgggcagac gtgaccacaa agcagacaac ccacacattt
     8641 ctcggtatta caatgcggtg tgcgggtttt ctcgctccat tccatcgcct ggtgaccgcc
     8701 gtcgtaacag gaaatataac agcgtccaca gccaacacat ttgtcaagat tgatacgggg
     8761 atagacaata taactgcggt ctaaatcttc cgccgggaca atattgttat tcgccagacc
     8821 taccatctcc tgcaacgaat caaatccctg atcggcgaga taatgcgata acccgctcgc
     8881 catatcttct acgatgcgat agccgtactg cataatgccg gtggtgacct gtaacgttgc
     8941 tgcgccgagc aataaaaact ccgccgcatc ttcccaggtt tcaatgccgc cgataccgct
     9001 gattgggaaa tcgcgcagtt ctggatgggt gcgcatttgc tgaatgaagc gcagggcgat
     9061 cggttttacc gctttaccgg aatatccgga aatactcgat ttaccgttaa cgatcggcat
     9121 accgattttc tgattaagat cgatattggt gatggattta acggtgttaa ttgccgcaat
     9181 gccatctgcg ccgccgcgct tcgccgccag cgccacttcg cacatatcac cgatattcgg
     9241 cgtcatcttc gccagcattg gcagcgtgga accccgtttc actgcccgac aatatttttc
     9301 taccagctcc gggctttgcc cgacatcgct acccatcgca tgagaagtca tttgcggaca
     9361 ggagaagtta cactcgatca tatccgcgcc agcttcttgc accaggcgcg ccagctcctc
     9421 ccattgctgc tcattttccc ccatgatcga agcgatcaat actttgtccg ggtaatcttc
     9481 cttcagccga cgcagggcgg tcagattctc ttccaacgga tgttcagcaa tctgctccat
     9541 atttttgaag ccgataaaac cggtatcttc tttcaccaga tgatcaaaac gcggcgagac
     9601 ttcgttggcg ataaaaaagc cgatcgtttt aaacaccaca ccgccccaac ctgtgtcgta
     9661 ggctttggca cacatctcat agcagttgcc taccggcgaa gaagagaggc agaacgggtt
     9721 gggaaagttc acgccgcaaa aagtaatcga aagatctttc gttaacatga gcaagctccc
     9781 tctaaatagt gatgaatcgc ctcggcggct tctttcccgg ttttcacggc atagaccacg
     9841 gttttgtcac cctcaacaat atcgccagca gcaaagactt gcggatcgcg ggtctgataa
     9901 ttttgtgttt tgatggtgtt acgctgctgc tctaactccg caaaggcatc cagtctggca
     9961 tgctgaccga cggcgagaat aattttatct gccgccatcg tcagttcgcc tgataaccgt
    10021 acatgcttaa acgtgacttt attgccttcc acggctactg gcgtgaatcc atcgatgatc
    10081 gaaacgccca gctcccgggc gctggcaaac tctttttcgc ttgccggaaa ctcatctaac
    10141 tcttcacgtg ctacgcaagt taccgcctga cagccgagaa ctttcagcgt gctggctacg
    10201 tccatcgcga catcaccgcc gccgataatt aatgcgcttt gtggaatgct gatatcgcct
    10261 tgcgcctgtc gtacacgttg caggaagtcg acggcaatct caacgtcgct atgctcaaac
    10321 agcggtagcc cggaaccgct tgataacccc acggtgacca gtaccgcgcg gttttccgct
    10381 ttcagttgct ccagggtgag tgttttaccg atttcgttat tgcacttaat ggtcacgccc
    10441 attttttcaa tacgggcgat ctctgcatcc agcactgatt gcggtaaccg gaattgcgga
    10501 ataccgttac gcaaccagcc accggggtgc gcttctttct cataaatcgt gacgtcataa
    10561 ccctggtttg tcagtgtcac actggcctgc aatccggcag gacctgcgcc aataatcgcg
    10621 actttgccga gcgttttagt accgggctga taaatttcca tcccggtttg ttgttcaaaa
    10681 tcagtaacaa aacgctgtaa gcggccgata tcaatgggag catcaacacc ggcacgggta
    10741 caaccgcttt ggcataattt ctccgtcggg cacactctgg cacaaacggc accgagggca
    10801 ttattttcgc gaattgtctc ggcagcgcct ttaaaattac gaaagtagat tgagcgaata
    10861 aatttccccg gatcggtctg tgccgggcaa gcctgactac agggagcgtc gtgacataat
    10921 aaacagcgag aggcttcttt aatcgccagt aacggcgtaa aagccggagt gagttcatcc
    10981 agataatttt gttgcggcat gtgatcttcc ttaatgctat gagtaattat ggcgaacgct
    11041 aaataaatat tgcgtgctga tattaaggct atgcgcgtgg ggaaatttaa actgtgaaag
    11101 gattcacaaa tagctgcggt tcagaatgtt atatccgcag cttactgagt gaagtaataa
    11161 ttattctcaa taattaacag aaaagagaaa atattaacga tagaaaataa gataaacaat
    11221 catgccgata gataaaattc cgaagccaat atatgccacc atataagcga agaagagtcc
    11281 gggatttttt gctcgccaga catacaccgg ttccggtgca tttttaaccc gtttatcaat
    11341 cgcgcctgcg cgccagcctt tccatgcttc gcggaaacaa aatatcggga taaggaaaaa
    11401 aacggcaacg acaaaaaact gctgtacatc cataactact ttccttgttt cttttgtaat
    11461 tcaagtaact gttcgggtgt aacggcggga tacccctgcg catcttccgg cacctggtgc
    11521 atagccggaa tagggaccag cggccctaaa aaacgcggtt cacgtttaaa aatataaagg
    11581 tcagccagcg caccgaaacg ggcggcaaat tcgcgaatac gtactgacag catatctttc
    11641 ggtgacggta cggcataaca ctgagcctga atccccatat gcagcgcaat aaataatgct
    11701 cgctcacagt ggaaacgttg ggtgataata atgaaatcat tagtatcgaa aactttgcgt
    11761 gtacgcacga tggaatccag cgtacgaaag cctgcgtaat cgagaacaat atctgatggg
    11821 tcgacaccag cagcgattaa atctttgcgc atggtcatcg gctcgttata actttgcaat
    11881 gcgttatcgc cgctcagtaa tagataattt accttaccgc tgttataggc attaatcgct
    11941 ccttgaatgc ggtagcgata atactgatta ataacgccgg tacgataata ttttgctgtg
    12001 ccgagcacca cgccgacctg gcggtagggg agatcctgca attcgtcgta gatataaggc
    12061 gcggttttcc agctcatcca gcgatcgagg ccgagcacag tcaacagcag caagccgatc
    12121 aggactaaca ggctgaggaa cacgcgcttt aacatgcagg agactcgagc taaggaaaat
    12181 ttccatcagg ctactttacc cgctggtttt gcgcaagaaa cggcaaactg tttgccggga
    12241 atttcgacac caggagaggg ctggcaagcc ggacaaggcg ttcacgccgc atccggcacc
    12301 aggcttaagc gagaagcacg cggtcgatac tgtgacagcc gagagctttc agcgttgcgg
    12361 aggtggcatc ccactgaatc aacggcgcat cggctttttc tgccagaacc gcgcgctgga
    12421 tatccgggta atcataaccc ttgagattca acagaattaa cgccccctgc aacggcggca
    12481 gagtcgggtt gaatgcggcg ttttcagcgt agctgccgct gaaaatacga ccgtctttac
    12541 attccagcgc gacaccgctt ggcgacttac tgtaaggcat gtgcgaacgg tttgccgctg
    12601 caatcgccgc ctgagaaagc gcatcaccag tcagcgcata gccgtgatcc tgctcgtcca
    12661 tcagcagcgt tttaatctcc agatctttcg gcccaaaggc atccggcaga tagtcacgca
    12721 gcgcgtgtgc ctcgcggccc ggcaaatgaa tacgcagatc cagaccgctg ttcagttcat
    12781 tcataaactg acggcagtga ccacaaggcg tatagttaac ggtgatggct gcaagcgctt
    12841 tttcaccact caaccaggcg tggctgatcg cgctttgttc ggcatgaacg gtttgctgca
    12901 ttgttgcacc aataaattcc atattggcac caaaatacca ggttccgctc acaccgcgcg
    12961 caattgcgcc aacattaaaa ttcgacaatg gcgtacgcgc acaggccgcc gccagcggaa
    13021 gtagtgcgaa tgccagcgcg tcttcgtcca gccccgttgc gctcttcagc gatgagactt
    13081 gctccccggt caacaaagcg gggaagtact tgtctgccag aataggttcc agtgcagatt
    13141 gcaagttatc cgcaagttgg gcaaaagcgg tttgaaaacg tggatgcatg gcgttgcctc
    13201 ataatcatgt aatgggataa cagtttacga acccgttatg gctttatatg tgatctgaat
    13261 ctcattaatt atgcaacacg tgaaacttac atcaaaaatg cgcgacgtat cgcaaatttt
    13321 agcccattac tgccagaata atcgggaaaa ggaacggtgc gatcagcgaa gtaattatcc
    13381 cgcataacac cagcgctagc gaactaaatg caccttcctg ataatccagc tcggcgcagc
    13441 gcgccgtacc gagggcgtgc gaggcagtcc ccattgccag accgcgcgca gctttggtac
    13501 gaatacgcat cgcattaagc aatgtatggc caaataccgc gccgaggatg ccgacgaaaa
    13561 tcacgcaaac tgcgctgatt gccggaatac cgccaatact gccgccaacc gccattgcaa
    13621 taggcgtggt gactgatttc ggcaggattg acgcggcgat ttccggtgaa gcgcccatca
    13681 acaatgccac ggaagtcccc gtcaccatcg caaccacgct gccgataaaa cagatggtga
    13741 tgatcgattt ccagcgcgcg cggatctggt gtagctgctc atataaagga taggccagcg
    13801 cgacgaccgc cggttgcagc agatcgttta gcacttcgct acctttaaag taactgtcgt
    13861 aagagatgcc ggtcagcatt aaaaaaggaa tgatcaccac cattgctacc agcaacgggt
    13921 taagcagggg aaatttatac cgtgccgcca gtttgcgggc ggcaaaaaag acaatcaacg
    13981 tcagcggtaa tgaccaccag atattcgcca tcattcttct gatcctttct gacctacgac
    14041 tttacgttca ccgtgtacca gttgtgaact ccagctcacc accagaaaaa ccaccagcgt
    14101 actgacagca caggaaacca ctaccgggcc aaactgcgcg cggagcaaat caaaatattg
    14161 catgacgcct acgccaatcg gcacaaacaa tagcgccata tagcgaatca gtatgtagca
    14221 ccccggattg acccatttcg ccggaagaat ttgcaaggcc agcaggacaa acaggatcag
    14281 catcccgatg atgctgcccg gaatggttac cggtagcagg gaagcaatga aaatgcctgc
    14341 atacaggcag gcataaatca ggacgaaagc gcgtaaatat tgccagataa tgttcagtgt
    14401 cttactcata atcaacaccc cagatgagat ggattcatca tacaattaat tcgtaaaacg
    14461 tgctaccgat cacattaaga aatcagcgct tattttttcg ctacccggta ttacgcaatg
    14521 agcagcgaac tcaggcatac atcaagcata gagagggatt gcagtttgca gcaatccctc
    14581 tttgcagacc tttaatccgc tgaccatagg cgctaacata gcgcctcatt tttttgcggg
    14641 tgatgatatg cgtgtgttac tggcaccgat ggagggagtg cttgactctc tggtgcgtga
    14701 attgctgacc gaagttaacg actacgatct gtgcatcacc gagtttgtcc gcgtggtgga
    14761 tcaactgctg ccggtaaaag tctttcatcg catttgccct gagctacaaa acgccagccg
    14821 gacaccatct ggtacgctgg tgcgcgtgca gttgttaggt cagttcccac aatggctggc
    14881 agagaacgcc gcccgtgcgg tggagttagg ttcctggggc gtggatctca attgcggctg
    14941 cccgtcgaaa acggttaacg gtagcggcgg cggggcgacg ttactcaaag atcctgaact
    15001 catctactag ggggcaaaag cgatgcgtga agctgtaccg gcgcatttgc ctgtcagcgt
    15061 gaaagtgcgt ctgggctggg acagcagtga gaagaaattt gaaatcgccg atgcggttca
    15121 acaggctggc gctacggagc tggtggtgca tgggcggacg aaagagcagg gttaccgcgc
    15181 ggagcatatt gactggcagg cgattggcga gattcgccag cggctgaata ttccggtgat
    15241 tgccaacggt gaaatctggg actggcagag cgcgcaacaa tgcatggcga tcagcggctg
    15301 cgacgcagtg atgattggtc gcggggcgct caatattccc aacctgagcc gggtggtaaa
    15361 atataacgaa ccgcgaatgc cgtggccgga ggtggttgct ttgctgcaaa aatatacccg
    15421 tctggaaaag cagggcgata ccgggttata tcacgttgcg cggattaaac agtggttgag
    15481 ttatttgcgt aaagaatacg atgaagcaac ggaattattt cagcatgttc gggtgttgaa
    15541 taactcccct gatattgcaa gggctattca ggaaattgat atcgagaaac tctaaaaacc
    15601 atagtgatgt ttttgacaat gtgttagctc tttatcaata ggccgcacgg ttgtttggtg
    15661 gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag cccacatgga tgtgggctga
    15721 gggcgcgttt tacagggatg ttacctcgcg cccgacccgg tagccgtaag ggataagccg
    15781 agggcaccgc gcagcggcga ttttgttcgc cagagcccgg gggtgcaggg ggcggcggcg
    15841 attggtcgcc ccctgcgcgc tccttgcgcc agtgattata tgaagcaatg cacatgacag
    15901 gagcgcaaca ggatgagaga ctgaaatatt ctcgttattt tcggatctga tgacattaat
    15961 taattttaaa atatcatctt aaatacgcca gtcaccacca gtagcccaat caggaaaata
    16021 atcaatacag cccagagtat aattttcatt ggcgttcctt tatttgtgag tgctgaataa
    16081 gtataggaaa aaacgaagcc ctttgccgtt atccataaac gattaagata aatcggaaga
    16141 tatcatttac ttagtctata tttttctggg tatgtcgttt tattgctact gatgtattgc
    16201 cacgcgaaac taaaaaatat atcgcaacac acggtaatct ccgcgcaccg tttcttacct
    16261 gattattccc ccatgaatcg tgattccttt tatccagcca tcgcctgttt tccgctgtta
    16321 ctgatgctgg ccgggtgtgc gcctatgcat gaaacccgcc aggcgttaag ccagcaaacg
    16381 cccgctgcac aagttgacac cgcattaccc acggcgctga aaaatggttg gccagacagc
    16441 caatggtggc tggagtatca cgataatcag ctcacttcat taattaacaa tgcactacag
    16501 aacgcaccgg atatgcaggt ggctgaacag cgcattcagc tagctgaagc ccaggcgaag
    16561 gcagttgcca ctcaggatgg tccgcagatc gacttttcgg cggatatgga gcggcaaaaa
    16621 atgtcggcag aagggctaat ggggccgttt gctctgaacg atccggccgc aggtacgacc
    16681 ggtccgtggt acaccaacgg aacttttggc ttaacggcgg gctggcatct cgatatctgg
    16741 ggaaagaatc gggcggaggt tactgcccgc ctgggtacgg ttaaagcacg ggcggcggaa
    16801 cgcgagcaaa cccgccaatt gctggctggc agcgtagccc gcctgtactg ggagtggcaa
    16861 acccaggcgg cgttaaacac ggtcttgcag caaatagaaa aagagcagaa caccattatc
    16921 gcgaccgatc gccagctata tcagaacggg attacttctt cagttgacgg tgtggaaacc
    16981 gatattaatg ccagcaaaac ccggcagcag ctcaacgatg tcgcggggaa aatgaaaatt
    17041 attgaggcac ggttaaatgc actcacaaat caccagacta agtcattgaa gcttaaaccg
    17101 gtcgcgttgc cgaaagtggc aagccagctt cctgatgaac tggggtactc cttactggcc
    17161 cggcgggcag atttgcaggc ggcgcactgg tacgttgagt catcgttaag cactattgat
    17221 gcggcaaaag cggcatttta tcctgacatc aacctgatgg ccttcctgca acaggatgcg
    17281 ttgcacttaa gcgatctgtt ccgtcattcc gcgcagcaaa tgggcgttac ggcaggcctg
    17341 acgctaccta ttttcgatag tggtcgtctt aacgccaatc tcgatatcgc aaaagccgaa
    17401 agcaacttgt ctatcgccag ctacaacaaa gcggtggttg aagcagtgaa tgacgtggcg
    17461 cgggcagcca gtcaggttca gacactggcg gagaaaaacc agcatcaggc gcaaattgag
    17521 cgcgatgcct tgcgtgtggt aggtcttgcg caggcccgct ttaacgcggg catcattgct
    17581 ggttcccgcg tcagcgaagc cagaatcccg gcgctgcgtg agcgggccaa tggcctgtta
    17641 ttgcaagggc agtggctgga tgcctccatt caactcaccg gtgcgctggg cgggggttac
    17701 aaacgctgat gatatgcgcc ttctatactt aacgtttatt cagcgttaag tggagaactc
    17761 gatggcatag gtcgcgataa ttaccgcttc cgattcgggg atcggcaaag agtgcgcgtt
    17821 attactggcg cagcaggggt ttgatattgg cattacctgg cactcagatg aagaaggggc
    17881 aaaagatacc gcgcgtgagg tggttaccca cggcgtacgt gcggagatcg tgcaactgga
    17941 tctcggcaat ctgccagaag gtgcgcaggc gctggagaca ctcatccagc gatttgggcg
    18001 tattgatgtg ctggtgaata atgcgggtgc aatgaccaaa gcgccgtttc ttgatatggc
    18061 ttttgatgag tggcgcaaga tttttaccgt tgatgttgat ggtgcatttt tatgctcgca
    18121 aattgcggct cgtcagatgg tgaaataagg gcagggcggt cgcatcatca acattacgtc
    18181 ggtacatgaa catacgccgc tgccggatgc cagcgcctac acagccgcta aacatgcgct
    18241 cggcgggtta accaaaacga tggcgctgga gttggtcagg cataagattt tggtgaacgc
    18301 agtcgcgtct ggggcgatag ccacgccaat gaacggcatg gatgatagcg acgtgaagcc
    18361 cgacgcggag ccttcgattc ctctacggcg ttttggcgca acgcatgaga ttgccagctt
    18421 ggtggtgtgg ctttgttcgg agggcgcaaa ttacacgaca gggcagtcgt tgatagtgga
    18481 tggcggcttt atgttggcga atccgcagtt caacccagaa tagcgcagtg aggtgcgtgg
    18541 atatttcaat gggttaggcc agataagaca ccagcgtcgc atcaggcatg gaacacccgg
    18601 tgccggatgt ggagtgaatg cttatctgtg gcaactgctt tacgcctgat tatccggctt
    18661 tttcttcccg cgccgtttca gccaccagcg cacgcccacc accagaacca cgaccagaat
    18721 caaccagacc cagtgcttca aatgctggtc gagattgtgc aaccacggcg caatcacctg
    18781 accaccagcg taaccaatag tggtaaaaat caacgcccag gcaaatgcgc cgagaatatt
    18841 cagcggcaga aagattttcg gcggcagctg gctggcacca atcagcgtcg ggccaatcac
    18901 ccgaaagcca tacataaagc gcgtaccaat gacaaacaga tacggatggc gttggataag
    18961 tttctgcgcc cgctcaattt tatcctgatg tttcgagaaa cggcgtaaca gcttgccgcc
    19021 aaaccgccgc ccgcacagat agagcacctg gtcgccaatc atgccaccaa gcgccacaga
    19081 aagtaccacc agtgggaact ttaatagccc ctgatgtgcc gcaacgcctc ccagcaaagt
    19141 cacggtttca ccttccgcca ggctaccgat aaccagcgcg gcataaccat attgtgagat
    19201 gagtgtattg agatccatag gccagtctgt ctccctttgg ttgcctgtaa gcatacaccc
    19261 tgtgaatcat tcacgctgaa aaaggacata catcagacgg tgcaaattac atggatgaac
    19321 aaaaaataat cacaagctgg cttatacttg aactgtcagg ttcatgaact cacaacaagg
    19381 gggcggtatg agccatgtct ggggactgtt ttcccatccc gatcgtgaaa tgcaggtgat
    19441 taatcgcgaa aacgaaacga tttctcacca ttacacccac cacgttttgc tgatggcggc
    19501 gattccggtg atttgcgcct tcattggcac tacacagatt ggctggaatt ttggcgatgg
    19561 cactatcctg aagttatcct ggtttaccgg actggcgctg gctgtcttgt tttacggcgt
    19621 tatgctggct ggggttgcgg tgatggggcg ggtcatctgg tggatggcgc gcaattaccc
    19681 gcagcgtccg tctcttgcgc actgcatggt cttcgcgggc tacgttgcaa ctccgctgtt
    19741 tttaagcggt ctggtggcgc tttatccact ggtctggttg tgtgcgctgg tcggcacggt
    19801 ggcgctgttt tacaccggct acctgcttta ccttggtatt ccgagcttcc tgaatatcaa
    19861 caaagaggaa ggccttagct tctccagttc gacgctggcg attggcgtgc tggtacagga
    19921 agtgctgctc gccctaaccg ttatactatg gggctacggt tatcgtttgt tctagaaagt
    19981 tctttgacgt tgcattgctg gcgtaaatgt cagcaatgca gcatttactc acgattctcc
    20041 tttgacgatc gcactttttg ctcgttatga tggcgtcgct agcctcagta aatccttact
    20101 ccggcggtgc gcaacccgtg cgcgtgaacc actatctgaa tgctcatcat gccgaaattt
    20161 cgagtttctt tatttagcct ggccctgatg ctggctgtgc cttttgcacc gcaggccgtt
    20221 gcgaaaacgg cagccgctac caccgcttca caaccggaaa ttgcctccgg tagcgcgatg
    20281 attgttgatc tgaataccaa caaagtgatc tattcgaacc acccggatct ggtgcgtccg
    20341 attgcgtcta tcagcaaatt aatgaccgcg atggttgtgc tggatgcacg actgccgctg
    20401 gatgaaaaac taaaagtgga tatcagccag acgccggaga tgaaaggggt ctattcgcgc
    20461 gtacgactga atagcgaaat cagccgtaaa gatatgctgt tgctggcgct gatgtcttca
    20521 gaaaaccgcg cggcggcaag ccttgcgcac cattatcccg gtggttacaa agcctttatt
    20581 aaggcaatga atgcgaaagc gaaatcgctc ggaatgaaca acacgcgctt tgttgaacct
    20641 accggattgt cggtgcataa cgtttcaact gcccgtgact taaccaaact gctcattgcc
    20701 agcaaacaat atccgttgat cgggcagtta agtactaccc gggaagatat ggcaaccttc
    20761 tctaacccga cgtatacgct gccgttccgc aatactaatc atctggtgta tcgcgataac
    20821 tggaatattc agttaaccaa aaccggcttt accaatgcgg cgggccattg tctggtgatg
    20881 cgtacggtta tcaataataa accggtggcg ctggtagtga tggacgcgtt tggcaaatat
    20941 acccattttg ccgatgccag ccgcctgcgt acctggattg aaaccggtaa agtgatgcct
    21001 gtgccggcag cagcgttgag ctataaaaaa caaaaagccg cccaaatggc ggcggcgggg
    21061 cagacggcac agaacgatta agtgaattcg gatggcgata ctctgccatc cgtaattttt
    21121 actccacttc ctgccagttt ttccgtttac tggttttacc gatccccgga ttcatgctat
    21181 tggtcggatc gttctcgcga tagaacttct gcaacgtctc cggtgcttta tacaaatgac
    21241 cgacgttatg ctcggcaggg tactgcgcgc cgcgctgctg tagcagttcc agcatctgtt
    21301 cttttaacgc atgcacatcc acgccttttt tcactatgta atcctgatgg aagacatagc
    21361 acataaaatg gccgtaatag agcttgtgca ccagctggct gtcgatctcc ggcggtaaat
    21421 gctcatacca ctcggtgtcg ttacgccgca gagcgatatc cagtgccaga atgtcttcga
    21481 cttcatcgga atgcaccgcc tgataacgaa ttgctgcgcc cgcagcggcg aaacggtgta
    21541 aaaacgcttt gctaccttcc tccggcgtac agacaaagaa atcgccctcg gcctgtttga
    21601 aataatccac cagccacgat ttggcttcac cgacgccatc gcctgccatt tttaacagca
    21661 gatgatgctc gtatttatcg cgccagtttt tcatgcgcgg cggtaaatgg ctggggaaca
    21721 ggtgaccgaa tttttgcatt gcacggtcag taaaatgcgg acggaagaat ttcactttct
    21781 ccagcatcgc atcggtgcgt cccttgagat taaagaagaa cggcatcttg tcggtgccta
    21841 acttatcaat catcaggaag gtgtctttgc cgtatttttc cgcaatatcg tagatatccc
    21901 ggtgcatata ttccccggca accggcagat tttcgaagtt agccagaata tgacggcgga
    21961 tttcggtcag cacttccggc tggttggtgc cgatataaaa cacctgctga tttttttccg
    22021 cttcgaaggt atcaagacgt accgcaaaga cggccagctt cccggcgcaa ccagaagatt
    22081 caaataaccg atccggatcg gcgttataac gtgcgggcgt gtcggcttca atatcacgaa
    22141 cgcggtggac atagtcataa tcgtgggcgt gacgaccatc gtgacgcaca tcgtcatctt
    22201 tgatgcgatc gtcatccagc ttgctaagga tctgctccgg cgtttcgccc agatcaatcc
    22261 ccagatggtt caccagcgtc agtttgccat cttcatctat acgcgcgaat aacgacattt
    22321 cggtatacgc cgggccacgt tgcaccagcg agccgccgga gttgttacaa ataccgccga
    22381 tgaccgatgc gcctatacac gatgatccaa tcaccgaatg cggttcgcgt cccagcggtt
    22441 tgagggcttt ttccagcgaa tagagcgtgg tgcccggata ggccagcacc tgttcgccct
    22501 tgccgagaac gtgcagcttg tcgagacgca gggtgctgat gatcacgata tcgcgatcat
    22561 agtcgttacc gtttggcgtc gatccttcgg tcaggcctgt attggcagcc tgcatcagaa
    22621 taattttgtc ggcggtgacg caggctttca gcacccgcca caattccagt agtgagccag
    22681 ggaaaacgac agccagcgcg tcgccctgac cagaacggaa gcccttgcga tagcgggccg
    22741 tttttgcggg atcggtgagc aggtgtgaat gaccgaccag acgggcaagt tcattcaaaa
    22801 aggctttatt atcagttgtt gtcatggaag acattttcca ctccttgtgg tggcgaaaaa
    22861 atatcacaaa ctagaatagc gccttgtatg acaattcagc gagtgaatat tttgaaaatt
    22921 cagacaataa actgagcatt ctttttctct ctccatcatg cttatggcaa actacggttt
    22981 ttgcaccatt ttcgctgcca cgtcgggcaa caaaggaaga aaaatccata tgaaatggct
    23041 atgttcagta ggaatcgcgg tgagtctggt cctgcagcca gcactggcgg atgatttatt
    23101 cggtaaccat ccattaacgc ctgaagcgcg ggatgcgttc gtcaccgatc tgctcaagaa
    23161 aatgacggtt gatgagaaaa ttggtcagct gcgcttaatc agcgtaggcc cggataatcc
    23221 gaaagaggcg atccgcgaga tgatcaaaga cggtcaggtt ggggcgattt tcaacaccgt
    23281 aacccgtcag gatatccgcg ccatgcagga tcaggtgatg gaattaagcc gcctgaaaat
    23341 tcctcttttc tttgcttacg acgtgctgca cggtcagcgc acggtgttcc cgattagcct
    23401 cggtctggcc tcgtctttta acctcgatgc ggtgaaaacg gtcgggcgtg tctctgctta
    23461 tgaagcggca gatgatggcc tgaatatgac ctgggcaccg atggtcgatg tctcgcgcga
    23521 tccgcgctgg ggacgtgctt ccgaaggttt tggcgaagat acgtatctca cctcaataat
    23581 gggtaaaacc atggtggaag cgatgcaggg taaaagcccg gcagatcgtt actcggtgat
    23641 gaccagcgtc aaacactttg ccgcatacgg cgcggtagaa ggcggtaaag agtacaacac
    23701 cgtcgatatg agtccgcagc gcctgtttaa tgattatatg ccgccgtaca aagcggggct
    23761 ggacgcaggc agcggcgcgg tgatggtggc gctgaactcg ctgaacggca cgccagccac
    23821 ctccgattcc tggctgctga aagatgttct gcgcgaccag tggggcttta aaggcatcac
    23881 cgtttccgat cacggtgcaa tcaaagagct gattaaacat ggcacggcgg cagacccgga
    23941 agatgcggtg cgcgtggcgc tgaaatccgg aatcaacatg agtatgagcg acgagtacta
    24001 ctcgaagtat ctgcctgggt tgattaaatc cggcaaagtg accatggcag agctggacga
    24061 tgctgcccgc catgtactga acgttaaata tgatatgggg ttgtttaacg acccatacag
    24121 ccatttgggg ccgaaagagt ctgacccggt ggataccaat gccgaaagcc gcctgcaccg
    24181 taaagaagcg cgtgaagtgg cgcgcgaaag cctggtgttg ctgaaaaacc gtctcgaaac
    24241 gttaccgctg aaaaaatcgg ccaccattgc ggtggttggg ccactggcgg acagtaaacg
    24301 tgacgtgatg ggcagctggt ccgcagccgg tgttgccgat caatccgtga ccgtactgac
    24361 cgggattaaa aattccgtcg gtgaaaacgg taaagtgctg tatgccaaag gggcgaacgt
    24421 taccagtgac aaaggcatta tcgatttcct gaatcagtat gaagaagcgg tcaaagtcga
    24481 tccgcgttcg ccgcaagaga tgattgatga agcggtgcag acggcgaaac aatctgatgt
    24541 ggtggtggct gtagtcggtg aagcacaggg gatggcgcac gaagcctcca gccggaccga
    24601 tatcactatt ccgcaaagcc aacgtgactt gattgcggcg ctgaaagcca ccggtaaacc
    24661 gctggtgctg gtgctgatga acgggcgtcc gctggcgctg gtgaaagaag atcagcaggc
    24721 tgatgcgatt ctggaaactt ggtttgcggg gactgaaggc ggtaatgcaa ttgccgatgt
    24781 attgtttggc gattacaacc cgtccggcaa gctgccaatg tccttcccgc gttctgtcgg
    24841 gcagatcccg gtgtactaca gccatctgaa caccggtcgt ccgtataatg ccgacaagcc
    24901 gaacaaatac acttcgcgtt attttgatga agctaacggg gcgctttatc cgttcggcta
    24961 tggtctgagc tataccactt tcaccgtctc tgatgtgaaa ctttctgcgc cgaccatgaa
    25021 gcgtgacggc aaagtgacgg ccagcgtgca ggtgacgaac accggtaagc gcgagggggc
    25081 cacggtagtg cagatgtact tgcaggatgt gacggcttcc atgagtcgcc ctgtgaaaca
    25141 gctgaaaggc tttgagaaaa tcaccctgaa accgggcgaa actcagactg tcagcttccc
    25201 gatcgatatt gaggcgctga agttctggaa tcaacagatg aaatatgacg ccgagcctgg
    25261 caagttcaat gtatttatcg gcactgattc cgcacgcgtt aagaaaggcg agtttgagtt
    25321 gctgtaatgc tttagtaagg gcgcgacgtt tgcgcccttt gcttgatgtt catatcttaa
    25381 cttgctgaat tttcgccctt tgtaggccgg ataaggcgct cgcgccgcat ccggcaggaa
    25441 acgccggacc gaatatgcct cttaagtatt tatctgattc tgcaactgaa tccttccgct
    25501 caggctaact ctgtcattat caactatgct tttctcttaa ttcgctgaaa acaacagtaa
    25561 aagaatgagg aaagcatcat gccactctta aagctctggg caggttcact ggttatgttg
    25621 gcagccgtga gcctgccgct acaggcggct tcccccgtta aagtcggttc aaaaatcgat
    25681 accgaaggcg cgctgctcgg caacatcatc ttgcaggtac tcgaaagcca cggcgtaccg
    25741 acggtgaata aagtgcaact cggaacgact cctgtggtgc gcggggcgat tacttccggt
    25801 gaactggata tctatccgga atataccggc aatggcgctt tcttctttaa agatgaaaac
    25861 gatgcagcgt ggaaaaatgc cgggcagggc tacgagaaag tcaaaaagct cgatgcggag
    25921 cacaacaagt taatctggct gacgcccgcg cctgcaaata acacctggac tatcgccgtg
    25981 cgtcaggatg tggcagagaa aaacaaactc acttcgcttg ccgacctgag tcgttatctg
    26041 caagagggcg gcaccttcaa actggcagcc tcggcagagt ttatcgaacg cgccgatgcg
    26101 ttacccgcgt ttgaaaaagc ctatggcttt aagctcggtc aggatcagtt gctgtcactg
    26161 gctggtggcg acacggcggt gacgattaaa gccgctgccc agcaaacctc tggcgttaat
    26221 gccgcaatgg cttacggcac tgacggtccg gtcgcggcgc tggggctgca aaccttaagc
    26281 gatccgcaag gcgtgcaacc tatctacgcg cctgcaccag tggtgcgtga atcggtgttg
    26341 aaggagtatc cgcaaatggc acag
//
LOCUS       DADLSB010000041        26311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.041, whole genome shotgun sequence.
ACCESSION   DADLSB010000041 DADLSB010000000
VERSION     DADLSB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.041"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1371
                     /locus_tag="JN331_001697"
     CDS             <1..1371
                     /locus_tag="JN331_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7259865.1"
                     /translation="DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQ
                     EARQRYISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTEL
                     AKLKYQTAQGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNE
                     AELLGYGQGERLRECMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYL
                     SERLKDQETFYAESDAQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNI
                     TEMLNGNKVEWRSWASSVLQEISKVLMNAAIVNGIRTAANSMSGAGGFIGSIGSWLGG
                     AVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLG
                     VRAVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRD
                     GGMLSG"
     gene            1364..1705
                     /locus_tag="JN331_001698"
     CDS             1364..1705
                     /locus_tag="JN331_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7259866.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            1705..2403
                     /locus_tag="JN331_001699"
     CDS             1705..2403
                     /locus_tag="JN331_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY7259867.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQSGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQLSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYMGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            2414..3157
                     /locus_tag="JN331_001700"
     CDS             2414..3157
                     /locus_tag="JN331_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7259868.1"
                     /translation="MTQTESAILAHTRRCAPAESCGFVISTPEGERYLPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMVVT
                     GFYRVPLSSAQPGDILLCCFGASVANHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWNTSAFTGIYNDLAAASACM"
     gene            3055..3735
                     /locus_tag="JN331_001701"
     CDS             3055..3735
                     /locus_tag="JN331_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY7259869.1"
                     /translation="MATTNAFCLASPPLEHICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRLQMNEGWYQIRIAGEDTAPEVVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSAMAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            3976..7444
                     /locus_tag="JN331_001702"
                     /pseudo
     CDS             3976..7444
                     /locus_tag="JN331_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            7513..8136
                     /locus_tag="JN331_001703"
     CDS             7513..8136
                     /locus_tag="JN331_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY7259870.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTVHLGGIVALSYASVKKSDTMKTGENTFHYESLHG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVEMVVIDLAYEGSGSGDWRTDGFIVGIGYKF"
     gene            8201..11014
                     /locus_tag="JN331_001704"
     CDS             8201..11014
                     /locus_tag="JN331_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7259871.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSASEAATRATDAADSARAANTSAG
                     QAATSAQEAFSSAGTASAKASEASKSAAAAESSKSAAATSAGAAKTSETNAAASVTAA
                     EKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASETRAESSKTAAAS
                     SASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAA
                     TRAETAAKRAEDIASAVALEDANTTKKGIVQLSSATNSTSETLAATPKAVKAANDNAN
                     GRVPSNRKVNGKALTADITLTPKDIGTLNSVTISFSGGAGWFKLATVTMPQASSIVYI
                     ALIGGAGYNVVSPQQAGISELVLRAGNGNPKGITGALWKRTAVGLTNFAWINTSGDTY
                     DIYVEIGNYATSVNIHWDCTTNASVSIYTSPTYSASKPSSVTDGVVYTMYSSHQKPTP
                     SDIGALPTTGGTISGPLSVTDGITGALKGNADTATKLAAAPKINGVKFDGSADINLTP
                     ENIGAFARRSTGAYADSDGAVPWNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKA
                     HYRNGGLFYRSSRDGYGFEEDRAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQG
                     QTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASST
                     DLGTKTTSSFDYGTKSSNATGDHNHSRGTMEITGTLGYFRSDSGNFYTASEAFTLGGS
                     APAHGFTGSQFTYGVPVNFNASRTWSGATSTTGNHAHSVPIGAHAHSVAIGSHGHTIT
                     VNAAGNAENTVKNIAFNYIVRLA"
     gene            11014..11598
                     /locus_tag="JN331_001705"
     CDS             11014..11598
                     /locus_tag="JN331_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7259872.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPLDIPAGFVAVFNSDESSWHLFEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIRDAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(11714..11962)
                     /locus_tag="JN331_001706"
     CDS             complement(11714..11962)
                     /locus_tag="JN331_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7259873.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADAWFVH"
     gene            complement(12024..13121)
                     /locus_tag="JN331_001707"
                     /pseudo
     CDS             complement(12024..13121)
                     /locus_tag="JN331_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            13210..14247
                     /gene="dusA"
                     /locus_tag="JN331_001708"
     CDS             13210..14247
                     /gene="dusA"
                     /locus_tag="JN331_001708"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY7259874.1"
                     /translation="MHDNHETQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFLGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            14381..14623
                     /gene="pspG"
                     /locus_tag="JN331_001709"
     CDS             14381..14623
                     /gene="pspG"
                     /locus_tag="JN331_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY7259875.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(14789..15772)
                     /locus_tag="JN331_001710"
     CDS             complement(14789..15772)
                     /locus_tag="JN331_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY7259876.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            15855..17270
                     /locus_tag="JN331_001711"
     CDS             15855..17270
                     /locus_tag="JN331_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY7259877.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            17323..18402
                     /gene="alr"
                     /locus_tag="JN331_001712"
     CDS             17323..18402
                     /gene="alr"
                     /locus_tag="JN331_001712"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY7259878.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRGAMKYVD"
     gene            18655..19848
                     /gene="tyrB"
                     /locus_tag="JN331_001713"
     CDS             18655..19848
                     /gene="tyrB"
                     /locus_tag="JN331_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY7259879.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(20071..20613)
                     /locus_tag="JN331_001714"
     CDS             complement(20071..20613)
                     /locus_tag="JN331_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259880.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FSYAKDFFLYIETQLKIAKDFCRPREEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
     gene            20976..21689
                     /locus_tag="JN331_001715"
     CDS             20976..21689
                     /locus_tag="JN331_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY7259881.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            21800..22216
                     /locus_tag="JN331_001716"
     CDS             21800..22216
                     /locus_tag="JN331_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAY7259882.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            22220..22576
                     /locus_tag="JN331_001717"
     CDS             22220..22576
                     /locus_tag="JN331_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7259883.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(22611..25433)
                     /gene="uvrA"
                     /locus_tag="JN331_001718"
     CDS             complement(22611..25433)
                     /gene="uvrA"
                     /locus_tag="JN331_001718"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY7259884.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTSGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            25687..26223
                     /gene="ssb1"
                     /locus_tag="JN331_001719"
     CDS             25687..26223
                     /gene="ssb1"
                     /locus_tag="JN331_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY7259885.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
BASE COUNT         6381 a         6467 c         7282 g         6181 t
ORIGIN      
        1 gataaacagc agagtgccct gacaaaaaca gagcgtgagc tggccctgtc cagactgaaa
       61 ggggaagaaa aagagcgtgt ccgactgggg tatgcggcgg atgacctcgg ttttgtgggt
      121 gatccgtatc aggaggcgag acaacgttat atcagtaatg ccctggaagc ctggcgcaat
      181 aacgaggcga ataaacccaa atcccggggt ggaaaatcag agacggaaaa agcggaagac
      241 agtttttccc ggctgctgaa gcagcagaaa gagcaattgg cacttgcggg gcagaataca
      301 gagctggcga agctgaaata ccagactgcg cagggcgaac tgaaaaccct gacggagatg
      361 cagaagcagg aactgctgcg caatgcggcc ctgattgacc agcaaaaaat ccgggagcag
      421 ttgcggtccc gggaagagac cctgaagaat gataatgtgg ctgcgcgtgc atcaaatgaa
      481 gcggaactgc tggggtacgg gcagggagag cggctccgtg aatgcatgcg ggagttgcag
      541 cagatccgcg acagtttccg ccagaaggat gcggaccttc agtctcagta tcagaccggg
      601 gatatcagtg aggattttta cagacaggct ctggcacaga acgcgcagta tctgagtgaa
      661 cgtcttaaag accaggaaac tttttatgcc gaatcggatg cgcagcgtgc ggactggcag
      721 aaagggctgc aggaggggtt cagtaactgg gtggataatg cgtccgatta cgcctcacag
      781 gcagcacagc ttgcgacgga gggtatctca gggatggtga ataacatcac ggagatgctg
      841 aacggaaata aagtggaatg gcgcagctgg gcctcatcag tgctgcagga aatatcaaaa
      901 gttcttatga atgccgcgat tgtcaacggg atcaggacgg cggcaaacag tatgtccgga
      961 gcgggaggat ttatcggcag tattggtagc tggctgggcg gtgcggtggc caatgcaaaa
     1021 ggcggcgtgt atacctcggc aaacctgagt gcgtacagca acagtattgt ggatacgccc
     1081 acgtactttg cctttgcaaa aggggcggga ctgatggggg aggccggtcc tgaagccatt
     1141 atgcccctga cccgggcggc ggatggctcg ctgggtgtgc gagcggtggg cagtatgaac
     1201 ggcagtgcag gtctggtgta ttccccggtc taccatatcg ccattcagaa tgacgggact
     1261 aatggccaga tagggccgga agctgcgggc agccttgtgc aactgattga ccagcgggtg
     1321 caggcggtga tgctgtccat gcgacgtgac ggaggaatgc tgagtggctg agataaaaac
     1381 gctgcatctg gtcccgcgtg aagggatgca ggtgagtgag aaaccgtcgg tggcgagggt
     1441 acggtttggt gacggttatg aacagcgccg cccgacggga cttaatgccc gactgaagac
     1501 gtttcaggcg gtgttccggg tgacggatga ggcgacccgg cgatggctgg aagagttttt
     1561 atcgtggcat ggtggttacc gtgccttttt gtggcgaccg ccgaaacata accggacggt
     1621 gagggtggta tgccgggagt ggagcgtcac agataacgcc aggtacagtg atttcagttg
     1681 tacgattgag caggtggtga actgatgcag gatattcacg aagaaagtct gaacgagtcg
     1741 gttaaatcag agcagtcacc gcgggtggta ctctgggaaa tcgacctgac ggtacagagc
     1801 ggtgagcggt attttttctg taatgagctg aatgaaaaag gggagccggt cacctggcag
     1861 gggcgtaagt atgaggcgta cccgattgat ggcagcggct ttgaaatgaa cgggaaaggc
     1921 agcagtgccc gcccgtcgct gacggtgtcc aatctgtttg gccttgtcac cgggatggcg
     1981 gaggatttgc agagtctggt gggtgccacg gtggtccgtc gccgggtgta tgcgcgtttt
     2041 ctggatgcgg tgaattttgt ggcgggcaat ccggaagcgg acccggaaca ggaactgagc
     2101 gaccgctggg tggtggagca gttatcagag ctgacggcca tgacggcctc gtttgtgctg
     2161 gcgacaccga cggagacgga cggggcgctg tttcccggtc gcatcatgct ggcgaacacc
     2221 tgtatgtgga cctaccgctc tgatgagtgt ggttacatgg gcggggctgt ggcggatgag
     2281 ttcgataaac ccaccacgga tatccgtaag gacagatgca gcaagtgcat gcgcgggtgt
     2341 gaactgcgca ggaatgtcgg caattttggc ggtttccttt ccattaataa actttcgcag
     2401 taaatcccgg tttatgacac agactgaatc agcgattctg gcacacaccc ggcgatgtgc
     2461 gcctgcggag tcttgcggct tcgtgataag cacgccggag ggggagcggt atctgccgtg
     2521 tgtgaatatc tccgcagagc cggaggcgta ttttcgtatc gcaccggaag actggctgcg
     2581 ggcagagatg cagggggaga ttgtggcact ggtccacagt catcccggtg agctgccctg
     2641 gctgagcgag gctgaccggc ggctgcagat aaaaagcgca ctgccctggt ggctggtctg
     2701 ccggggggaa attcataaat tccgctgtgt gccacatctg acaggacggc gctttgagca
     2761 cggggtgacg gactgttaca cgctgttccg ggatgcatac catctggcgg gaattgatat
     2821 gccggatttt catcgcgagg atgactggtg gcgcaacggc cagaacctgt acctggacaa
     2881 tatggtggtc accggctttt accgggtgcc cctgtcctct gcacagccgg gcgatatcct
     2941 gctgtgctgc ttcggcgcat cggtggctaa tcatgccgcc atttactgcg gcaacggtga
     3001 actgcttcac catattcctg aacaactgag taaacgggag aggtattccg aaaaatggca
     3061 acgacgaacg cattctgtct ggcgtcaccg ccactggaac acatctgcct tcacggggat
     3121 ttacaacgat ttggccgccg cctcagcctg tatgtgaaca cggcagcgga agccattcgc
     3181 gccctgtcga tgcagatgcc gggctttcgc cttcagatga acgaaggctg gtaccagata
     3241 cgtattgccg gtgaagacac ggcaccggag gtggtgtacg cccgccttca cgaacagctg
     3301 ggtgagggaa cggtcatcca cattgtgccg cgactggccg gggccggaaa gggtggactg
     3361 cagattgtgt tgggggcggc agccatcgtg ggctctttct tcactgccgg ggcatcaatg
     3421 gcgttatggg gttcagccat ggcagccggt ggtttttctg ccaccacgat gctgttttca
     3481 cttggagcca gcatgattct gggcggtgtg gcccagatgc tggccccgaa ggcaaaaaca
     3541 ccggattacc gcgcaacgga taacggcaga cagaacacgt acttttcctc gctggataac
     3601 atgattgccc aggggaaccc gatgccggtg ccttacgggg aaatgctggt tggctcccgc
     3661 cgtatatccc aggacatcag cacccgtgat gaaggcgggg gcggaaaggt cgtggttatc
     3721 gggcggcagg gataaaacat aaaaaaatcc cgcagtgatc gcggagctgc ggggacagac
     3781 aaatgaagat caatgttaag gagttgtttt tgttactcgg gcaaaaaaac actaacgcag
     3841 cgaaattata agcgccacag tcagtgtgtg aaaatgtgaa gatattcaga aattttattc
     3901 cgtcatgacg caggcacccg gtgaggtgcc tgttgttttt gtgagtgaac aattatcacg
     3961 gtaagaggtg atgtaatggg caaaggtggc ggcagggcgc acacaccgcg tgaggcgaaa
     4021 gacaatctca aatccacgca gatgatgagc gtgattgatg cgattggtga gggaccgata
     4081 gaaggcccgg tgaaaggcct gcagagtatt ctggtgaaca aaaccccgct gacggacacg
     4141 gacggtaatc ccgtgataca cggtgtgacc gcggtctggc gtgccgggga gcaggagcag
     4201 acaccaccgg aaggctttga gtcctctggc tctgaaactg tactgggtgt cgaagtgacc
     4261 agggcaaaac cggtaacacg caccattacg tcagcgaaca ttgaccgcct gcgggtgacc
     4321 ttcggggtgc agtcactggt ggagaccacg tcaaagggtg accgtaatcc gtcctctgtc
     4381 cgtctgctga ttcagttaca gcgtaacggt aactgggtga cggaaaagga tgtcaccatt
     4441 aacggcaaga ccacctcaca gttccttgcg tcggtgattc tggataatct gcctccccgc
     4501 ccctttaaca tccggatggt cagggagacg gcggacagca ccacggacca gctgcagaac
     4561 agaacgctgt ggtcgtcata caccgaaatc atcgatgtga aacagtgcta cccgaacacg
     4621 gccattgtgg ggatgcaggt ggatgcggag cagtttggtg gtcagcagat gacggtgaac
     4681 taccatatcc gcggtcgcat catccaggtg ccgtcaaact atgacccgga aaaacgcacg
     4741 tacagtggta tctgggacgg cagtctgaaa ccggcataca gcaacaatcc ggcctggtgt
     4801 ctgtgggaca tgctgactca cccgcgctac ggcatgggaa aacgtctggg ggcggcggat
     4861 gtggacaagt gggcgctgta tgccatcggg cagtactgcg accagatggt gccggatggc
     4921 ttcgggggca ccgagccgcg gatgaccttc aatgcgtacc tggcacaaca gcgtaaggcg
     4981 tgggatgttc tcagtgattt ctgctcggcg atgcgatgta tgccggtatg gaacggtcag
     5041 acgctgacgt tcgttcagga ccgcccgtcg gatgtggtgt ggccgtacac cagcagcgat
     5101 gtggtggtgg atgataacgg cgtggggttc cgctacagct tcagtgccct gaaggaccgg
     5161 cacacggcgg tggaggtgaa ttacaccgac ccgcagaacg gctggcagac ctccacggaa
     5221 ctggtggaag acccggaagc catactgcgc tacgggcgca acctgctgaa gatggatgcg
     5281 ttcggctgca ccagtcgcgg tcaggcccac cgtgccggac tgtgggtgat aaagaccgga
     5341 ctgctggaaa cgcagacggt ggatttcacg ctcgggtcac aggggctgcg tcacacaccc
     5401 ggtgacatta ttgaaatctg tgataacgac tatgccggga ccatgaccgg cggacgtgtc
     5461 ctgtccatcg atgccgccac ccgcaccctg acgctggacc gtgaggtgac actgccggag
     5521 accggtgccg ccacggtgaa cctgattaac ggcagcggta agccggtgag tgtggacatc
     5581 accgcacacc ccgcgccgga ccggatacag gtcagtaccc tgccggatgg cgtggagaca
     5641 tacggtgtgt ggggactctc cctgccgtca ttgcgccgtc gcctgttccg ctgtgtctcc
     5701 gtccgggaaa acacggacgg cacctttgcc atcacggcag tgcagcatgt gccggagaaa
     5761 gaagccatcg tggataacgg ggcgcacttt gacggcgacc agagcgagcg tcatccctcc
     5821 ggcagtgcag cacctgacgg tggaggtgag cgcagctgac ggccagtatc tggcgcaggt
     5881 gaaatgggac acgccgcggg tggtgaaggg cgtgcgcttc agtctgcgac tgaccagcgg
     5941 aagcggagaa ggcagccatc tggtgaccac cgccatcact gcggatacag agcatcgttt
     6001 cagtggtctg ccgctcgggg aatacaccct gacagtcagg gcaattaaca gttatggcca
     6061 gcagggcgaa ccggccacca ccaccttccg gattaacgcg ccagcaaaac ccgccaccat
     6121 tgagctgacg ccggggtatt ttcagataac ggcggtccca cgtcttgcgg tgtatgaccc
     6181 gacggtacag tttgaattct ggttctcaga aaaacgcatc acgaacacag cacaggtgga
     6241 aaaatctgcc cgttatctgg ggaccggcag tcagtggact gtctccggcc cgcacattaa
     6301 gcccgggaag gatttctggt tttatgtacg cagcgttaac ctagtgggga aatctgcttt
     6361 tgttgaagcc agtggacggg ccagcaatga tgcagaaggg tatctggggc tgtttcggga
     6421 aaaaatagga aaactgcatc tggctcaggg gctgtgggag ctgatagaca acagccagct
     6481 tgcggatgag atggcggaga tgaagaccac catcaccgaa acccgcaatg aaatcacaca
     6541 gacggtcagt aaaacgctgg aagaccagag cgccaccatt cagcagatac agcgcgtgca
     6601 gaaggacaca aatgatgacc tggctgcgct gtacatgctg aaggttcaaa aaacgaaaga
     6661 cggcattccc tatgtggccg ggattggtgc agggattgag gatactgatg gccagccact
     6721 gagcaacata ctgctgctgg ctgaccgtat cgcgatgata aatccggaga acggcaacag
     6781 cacgccgtta tttgtggcgc aggggaatca gctgttcatg aacgacgtgt tcctgaagcg
     6841 actgtttgcg gtgagtatca cgtcatccgg caaccccccg acgttttccc tgacgccgga
     6901 agggaagctg acagccagga acgcggatat cagcggagca attaccgcga ataccggcac
     6961 gctcaataat gtcaccatta acgagaactg tgtcatcaga gggaaactgt ctgcaaacca
     7021 gattgaaggc gacctggtga agacggtggg gaaagccttt ccccggaata acagttatgc
     7081 cagcgggacg gtaactgtca cagtttacga tgaccaggga ttcgaccggc agattatcat
     7141 tcccccggtg ctgtttcgcg gggcgaaaca ccagaatttc aacagcccga atcagcagtc
     7201 gtactggtat tccacctgta agctgcaggt gctgaagaac ggggttgaga ttttccatga
     7261 accggcaacg gatgtcagcc gggtgttctc atcggtgata gatatgccgg cagggcaggg
     7321 tcatgtcacc ctgacgttta atgtgtcgtc ggccggtgcg aacaactgga cgccgacaac
     7381 gtacatcagt gatttactgg ttgtggtcat gaaaaaatcc acggcaggga tcagtatcag
     7441 ctgacggttt attaacccgg acgggcacct caggaggtgc cttttttatt gactgaaaac
     7501 aaagaggtaa tcatgcggca tttatacgca acgatattat tgtttactac cctgctggca
     7561 ggaattgcct ttcctgcaca ggctgaaagc ggacacggtg cattttccgt gggatatgct
     7621 caggttcacc cgggcggcgt accggcattg tccggtaccg gtgcgcgtgc aggtgattta
     7681 aaagggatta atgtgaaata ccgttatgag ttcacggttc acctgggcgg cattgtcgcg
     7741 ctgagttatg catcggtgaa gaaaagtgac acgatgaaga cgggtgaaaa taccttccat
     7801 tatgaaagcc tgcacggtcg ttatgtcagt ctgatggccg gccctgtctg gcagctcagt
     7861 gagcgggtca gtctctatgg catggccggg atggcgtaca cccgctggtc tgacagtgtt
     7921 caggattacc ggcgtgatga agtaaaaccg gggtatgtga aggagaccac caccgccagt
     7981 gatggtcata ctgcgcgtca tctgtcgccg gcctggaatg ccgggattca gttcagtccc
     8041 gtagagatgg tggttattga ccttgcttat gaaggttccg gcagtggcga ctggcgcact
     8101 gacggtttca tcgtgggtat cggttataaa ttctgattag ccaggtaaca cagtgttatg
     8161 acagcccgcc ggttcaggcg ggcttttttg tggggtgaat atggcagtaa agatttcagg
     8221 tgtactgaaa gacggcacag gaaaaccggt acagaactgc acaatccagc tgaaagcaaa
     8281 acgtaacagc accacggtgg tggtgaacac gctggcatct gaaaatccgg atgaagccgg
     8341 gcgttacagc atggacgttg agtacggtca gtacagcgtt attctgttgg tggaagggtt
     8401 cccgccgtca catgccggga ccatcaccgt gtatgaagat tctcaacccg gtacgctgaa
     8461 tgattttctt ggtgccatga ctgaggatga tgtccgtccg gaggcactgc gccgttttga
     8521 actgatggtg gaagaggtgg cgcgtaacgc gtccgcagta gcacagaaca cggcagccgc
     8581 gaagaagtca gccggcgatg ccggcacatc tgccagtgag gcggcaaccc gtgcgactga
     8641 tgctgcagat tcagcacgtg cagccaacac gtcagccgga caggccgcga cgtcggctca
     8701 ggaggctttt tccagcgcag gaacggcatc agcaaaagcc tctgaggcat cgaaaagtgc
     8761 tgctgctgca gagtcctcaa aaagcgcggc ggccaccagt gccggtgcag cgaaaacgtc
     8821 agaaacgaat gcggcagcca gcgttaccgc cgctgaaaaa tcggcagaaa gcgccgcatc
     8881 gtccgcttca acagccacaa cgaaggctgg cgaagccact gaacaggcca gcgcagcagc
     8941 gaggtctgct tccgcagcga agacatccga aacgaacgcg aaagcgtcgg aaacccgtgc
     9001 agaatcctca aaaacggctg ccgcatcatc cgccagttcg gcggcgtcat cggcatcatc
     9061 tgcgtctgct tcaaaagatg aggcgaccag acaggcgtca gcagcgaagg gcagcgccac
     9121 gacggcatcc acaaaggcga cagaggctgc tggcagtgcg acagcggcag cacagagcaa
     9181 aagtacggca gaatctgcag caacgcgcgc tgagacagcg gcaaaacggg cagaggatat
     9241 tgcatccgcc gtggcgcttg aggatgcgaa cacgacgaaa aaggggatag tccagctcag
     9301 tagtgcaacc aacagcacat ctgaaacact tgccgcgaca ccgaaggcag ttaaagcagc
     9361 gaatgacaat gcgaatggca gggtaccatc taaccgaaaa gttaacggga aagcactgac
     9421 tgcggatata acattaacac cgaaagatat tggcacttta aattcagtaa cgatctcttt
     9481 ctctggcggg gcagggtggt tcaaactggc aacggttacc atgccacaag cgagttccat
     9541 cgtttacatc gcattgattg gtggcgctgg ttacaacgtc gtctccccac agcaggcagg
     9601 catttcagaa ctggttctac gagcaggcaa tggaaacccc aaagggatta ccggagcttt
     9661 gtggaagcgt acagccgtcg gattaacgaa tttcgcctgg atcaacacat ccggcgatac
     9721 atatgatatt tacgttgaga ttggcaatta tgcgacgagt gtaaatatcc attgggattg
     9781 tactacaaat gcgtcagttt ctatttatac ctcgccaaca tattcagcga gtaagccttc
     9841 cagcgttacc gatggtgttg tttatacgat gtatagctca catcagaaac ctacaccatc
     9901 agatattgga gcgctgccaa cgactggagg gactatttca ggtccgttgt ctgttactga
     9961 tgggatcacc ggggcactga aggggaacgc cgataccgcg acgaaacttg cggcagcccc
    10021 aaaaattaac ggtgttaagt ttgatggctc ggcggatatt aacctcacgc cggaaaatat
    10081 tggtgcattt gcccgacgtt cgacgggggc ttatgcggat tcggatggag ccgttccctg
    10141 gaatgccgaa tcaggcgctt acaatgtcac ccgctctggc gacagctata ttctggttaa
    10201 cttctatacc ggagtcggaa gttgccggac cttgcagatg aaggcgcatt acagaaatgg
    10261 tggtctgttc taccgttctt caagagacgg ttatggtttt gaggaagaca gggcagaagt
    10321 ttatacctcg aaaaatcttc caccagaaag ctacccagtc ggcgcaccaa tcccgtggcc
    10381 atcagatacc gttccgtctg gttatgccct gatgcagggg cagacttttg acaaatctgc
    10441 ctacccgaaa cttgcagccg cttatccgtc aggcgtgatc cctgatatgc gtggctggac
    10501 gattaagggc aaacctgcca gtggtcgtgc cgtattgtct caggaacagg acggcattaa
    10561 atcgcacacc cacagcgcca gtgcatccag tacggatttg gggacgaaaa ccacgtcgtc
    10621 gtttgattat ggtactaagt cttcaaatgc cactggtgac cataaccaca gccgaggcac
    10681 tatggaaatt accggtactc tcggttactt cagaagtgat agcggtaatt tctatacagc
    10741 aagtgaagca tttacactgg gtggctctgc gccagcccat ggatttacgg gctctcaatt
    10801 tacttatggt gttcctgtaa actttaacgc ttccagaacc tggtctggtg ccacaagcac
    10861 tacaggtaac catgcgcact ctgttccaat tggtgctcat gcgcactccg ttgcgattgg
    10921 ttcacatgga cacaccatca ccgttaacgc tgctggtaac gcggaaaaca ccgtcaaaaa
    10981 catcgcattt aactatattg tgaggcttgc ataatggcat tcagaatgag tgaacaacca
    11041 cggaccataa aaatttataa tctgctggcc ggaactaatg aatttattgg tgaaggtgac
    11101 gcatatattc cgcctcatac aggtctgcct gcaaacagta ccgatattgc accgttagat
    11161 attccggcag gcttcgtggc tgttttcaac agtgatgagt catcgtggca tctctttgaa
    11221 gaccatcggg gtaaaacggt ttatgacgtg gcttccggcg acgcgttatt tatttctgaa
    11281 ctcggtccgt taccggaaaa tgttacctgg ttatcgccgg aaggggagtt tcagaagtgg
    11341 aacggcacag cctgggtgaa ggatacggaa gcagaaaaac tgttccggat ccgggatgcg
    11401 gaagaaacaa aaaacaacct gatgcaggta gccagtgagc atattgcgcc gcttcaggat
    11461 gctgcagatc tggaaattgc aacggaggaa gaaacctcat tgctggaagc ctggaaaaag
    11521 tatcgggtgt tgctgaaccg tgttgataca tcaactgcac ctgatattga gtggcctacg
    11581 aaccctgtca gggagtaatc attgggatta tgccgcagca cgtcttaagc aagaacgtgc
    11641 tgcggttgga tggtgaactt tcgatagtgc gagtattgaa tgatttccag ccgttaccga
    11701 ttttacgtgt taattagtga acaaaccacg cgtcagcaga ctcccaggta tctttcagag
    11761 tttcctgaac aaaagtttta gctgaatctt tatcggcggt gcgcgtaaca gaaaggccat
    11821 cgttgctggt agcttttacg atcacctcta catcgtcata acgtttactg atgcgtcggg
    11881 ttaattcttc ctttaacgca tccacagcac cggttggcat tttagtcatt ttttctttgg
    11941 ctatgcagat ttcaatacgc ataaaagtcc ctctatactg tgtttgtata cagtattatt
    12001 tttaactgta tggataaaca gtgtcaagag atcttatttc tgctcctttg gagctcttca
    12061 aaacgattat gtaaagattt cggatacagt tcggtatata cctgccatag cacgtttaat
    12121 gaacgatgcc ctgtaacctg ggctacttcc tcaatactaa aaccagcctc aaataagcga
    12181 cttgcccctt ctctacgcaa atcatggtat cgcagatcct taatacctaa tttgcttctt
    12241 accctctgaa atcccgcagt aacagaagtg ctgttatatg gaaaaatgaa ttccgatttt
    12301 tggggctgtc gttggacgat atcccaggct tccccaagca aggctacttt catgtggttg
    12361 ccttcctttt tgcgtggatc tttcctgtct cttacgagta tagatttttg ttcctggtcg
    12421 agatcttctc atcgtaaccg gcatacttct ccgattcgca tacaggacca cacagaaaat
    12481 ttgaggatat caacgaacgg aatttttgag catttatgag tagatcgttg ttgaaggcct
    12541 tcaatgagca tgtccagttc atcagatgct ggtctacgat tacgacggtt tgatttacca
    12601 atcaaaccaa gtttaagtag gtatggacga gcacttttcg ccgggtttga tgtgtaatta
    12661 attccgtata caggtttggc cgcatccaga acactgccaa gataactaac atcgtggctg
    12721 actgttgctg gacttgcgcc agcgttgttt cttagcctgc aatgttcaat tacgtcattt
    12781 tctgtcagtt cagatagttt gatcgcggag atgtcactat ccataagcag ttccagcaca
    12841 tatcttttag tacggcctgc tttacctccg gcatttgggt catttaaata tttgtgtagt
    12901 aagtcacgga ctgtaagtcc gtcaactgca tttgatgatg gaattccata tagatctaat
    12961 tccatcactt tctgtgtgcc ccatgttttg gcatgagcat gtttagggaa tgttttgctt
    13021 tccctgtaag tgataacacc tttttctttg ataatcacat tacagcgata gcgtggtgtg
    13081 ccatcggatt ttagtcgttt ctctatgtta tagtacgcca ttacacgacc tcgttatttc
    13141 gggttcccat aaaacgtggg aacctgtgcg ggaacctgac gcgagaaaaa tagcctgaaa
    13201 tgttcaaaaa tgcacgataa tcatgaaaca caaaaaatta atcaaaccag cgtgatgcct
    13261 gaaaaaactg gtgtttactg gaattctcgg tttagcgttg caccaatgct cgactggacg
    13321 gacagacatt gccgctattt cttgcgtctg ctttcccgca atacgttgct ttataccgaa
    13381 atggtgacca caggggcgat tattcacggt aaaggtgatt atctggcgta cagtgaagaa
    13441 gaacatccgg tagcgttgca actgggcggt agcgatccgg cggcgctggc gcagtgtgca
    13501 aagctggcag aagcgcgcgg atatgatgag atcaacctga atgtcggctg cccgtctgac
    13561 cgggtgcaga acggcatgtt tggtgcgtgt ctgatgggta atgcgcagct ggttgccgac
    13621 tgcgtgaaag cgatgcgcga tgtggtgtcg attccggtga cggtgaaaac gcgtattggc
    13681 atcgacgacc aggacagcta tgaatttctc tgcgatttca tcaataccgt ttccggcaaa
    13741 ggcgagtgtg agatgttcat catccacgca cgtaaagcct ggctttcggg gttaagcccg
    13801 aaagaaaacc gtgaaatccc gccgctcgat tatccgcgtg tgtatcaact gaagcgtgac
    13861 tttccgcatc tgacaatgtc gattaacggt ggtatcaagt cgctggaaga agctaaagcg
    13921 catttgcaac atatggatgg cgtgatggtc gggcgcgagg cgtatcagaa tccgggtatt
    13981 ctggcggcgg tagaccggga gatctttggt tcctcggata ccgatgccga tccggtggca
    14041 gtagtgcgtg ccatgtatcc gtacattgag catgaactca gccaggggac gtatctcggc
    14101 catattatcc ggcatatgct gggcttgttc ctgggtattc ctggcgcgcg gcagtggcgg
    14161 cgttatttaa gtgaaaatgc ccataaagcg ggtgctgaca ttaacgtgct ggaacacgcg
    14221 ctcaaactgg tggcggataa gcgttaactt ttcaccaaaa agtagtcaaa ttcaccacgc
    14281 cctgcgcacc gtcgcggggc gttttgctgt taaatcaata gattattttt ggcatgattc
    14341 ttgtaatgcc agcaagagat ttcatatttg ggagagcatc atgctggaac tactttttgt
    14401 gattggcttt tttgtcatgc tgatggtcac cggcgtttcg ttgctgggca ttatcgccgc
    14461 gctggttgtg gcgacggcca ttatgttcct cggcggtatg ctggcattga tgattaagtt
    14521 gctgccgtgg ttactactgg cgattgcggt ggtgtgggtt attaaggcga ttaaagcacc
    14581 aaaaatgccg aaatatcagc gttatgaccg ctggcgttac taagggattg tgcggatgat
    14641 cacaacctaa ggttttatcc ttagaacaaa ataggaattg ataatcaaat ctgtcactat
    14701 tgcgcctcta acagattcat cgtgctgtac cctacataca gccgaactat aaaaagaaag
    14761 ggcttcccag gtggaagccc tatttctttt atggaatcag caggctggaa ccttgcgtcg
    14821 cccggctttc cagaatctca tgcgcacgct gcgcatcctt cagcggatat ttctgctgct
    14881 cggcgacatc gaccttaatc acaccgctgg caatcaaaga gaacagttca ttactggcct
    14941 cggttaattc ctcccgcgtg gtgatatagc cttgcaggga agggcgtgtc acatacaacg
    15001 agcctttttg attgagaatg cctaagttca caccggtaac cgcacctgat gagttgccaa
    15061 aactgaccat taagccgcgg cgttgcaggc aatccagcga ccgttcccag gtgtctctgc
    15121 ccacggaatc gtacactacg cgcactttct taccgccggt gatctctttc aatcgctcga
    15181 ccagattctc ttcacgatag ttaataacct gccacgcgcc ggcttttagc gcgctctgcg
    15241 ctttttgcgc ggttcctacg gtgccgatga gtttcgcgcc cagggctttt gcccactggc
    15301 aggcaattaa gccaacgccg ccagccgctg cgtggaacag gaactgttca tcgggtttaa
    15361 tttcataggt tttgcgcagc agataataaa ccgttaagcc tttcaggaag gatgccgcag
    15421 cttgctcaaa agaaattgcc gcaggcagaa tcgccgcttt atccgcatta atgttatgca
    15481 cagagctgta agcgcctaac gccgactgcg catagactac atgatcgcca gccttaatat
    15541 gctttacacc actgccgact ttactcacga tgcctgccgc ttcggtacct aatccgctgg
    15601 gtagcgatgg cggcgggtaa aggccgctgc ggatatacgt gtcgataaaa ttgatgccga
    15661 tggctttatt ttcgacctgg atttcattct ccgccggatc ggcaggagtg aactctacgg
    15721 cttgaagtac ttccgggcca ccgtgcttgt gaaattcaat tcgtgttgcc atgtgtcctc
    15781 cagaacgtat cgtcagggtc tgcttcatat ggtaaagttt cgacccattc tttatctcgg
    15841 taactccatt cactatggca ggaaataaac ccttcaacaa acagcaggct gaaccccgcg
    15901 aacgcgatcc acaagttgcc gggctgaaag tgcctccgca ctcgatcgaa gcggagcagt
    15961 cggtgttggg cggtttaatg ctggataacg aacgctggga tgatgtagcc gagcgtgtgg
    16021 tagcagacga tttttacacc cgcccacacc gtcatatctt tactgaaatg gcgcgtttgc
    16081 aggaaagcgg tagtcctatc gatctgatta cccttgcgga atcgttggaa cgccaggggc
    16141 aactcgatag cgtcggtggt tttgcttatc tggcagagct gtcaaaaaat acgccaagtg
    16201 cggcgaacat cagtgcttat gcggacatcg tgcgtgaacg tgccgttgtc cgtgagatga
    16261 tctcggttgc gaatgagatt gctgaagccg gttttgatcc gcaggggcgt accagcgaag
    16321 atctgctgga ccttgctgaa tcccgcgtct ttaaaattgc cgaaagtcgt gcaaacaaag
    16381 acgaagggcc gaagaacatc gccgatgtgc tcgacgcaac cgtggcgcgt attgagcagt
    16441 tgtttcagca gccacacgat ggcgttaccg gggtaaacac cggttatgac gatctcaaca
    16501 aaaaaaccgc tggcttgcag ccgtcggatt tgatcatcgt cgccgcgcgt ccgtcgatgg
    16561 gtaaaacaac atttgcgatg aacctcgtcg aaaacgcggc aatgttgcag gataaaccag
    16621 tacttatctt ctcgctggag atgccatcag aacagatcat gatgcgttct ctggcgtcgc
    16681 tgtcgcgcgt tgaccagact aaaatccgta ccgggcagct cgatgatgaa gactgggcgc
    16741 gcatttccgg caccatgggt attttgctcg aaaaacgcaa tatctatatc gatgactctt
    16801 ccggcctgac gccaacggaa gtgcgttccc gcgcacgccg tattgcccgt gaacacggcg
    16861 gcatcgggct tatcatgatc gactacctgc aactgatgcg cgtaccggcg ctttccgata
    16921 accgtacgct ggaaattgca gaaatctccc gctcgctgaa agcactggcg aaagaactga
    16981 acgtgccggt ggtggcgctg tcccagttga accgttctct ggaacaacgt gccgacaaac
    17041 gcccggtcaa ctccgacctg cgtgaatctg gctctatcga gcaggatgcg gacttgatca
    17101 tgtttatcta tcgtgatgag gtgtatcacg aaaacagtga tttaaaaggc atcgcggaaa
    17161 ttattatcgg taaacaacgt aacggcccaa tcgggacggt acgcctgacc tttaacggtc
    17221 aatggtcgcg cttcgacaac tatgcggggc cgcagtacga cgacgaataa taattatttt
    17281 atgaattagg taattaaagc aaacacttat caaggaacac aaatgcaagc ggcaactgtt
    17341 gtgattaacc gccgcgctct gcgacacaac ctgcaacgtc ttcgtgaact ggcacctgcc
    17401 agtaaaatgg ttgcggtggt gaaagcgaac gcttatggtc acggtcttct tgagaccgcg
    17461 cgaacgctcc ccgatgctga cgcctttggc gtagcccgtc tcgaagaagc tctgcgactg
    17521 cgtgcggggg gaatcaccaa acctgtactg ttactcgaag gcttttttga tgccagagat
    17581 ctgccgacga tttctgcgca acattttcat accgccgtgc ataacgaaga acagctggct
    17641 gcgctggaag aggctagcct ggacgagccg gttaccgtct ggatgaaact cgataccggt
    17701 atgcaccgtc tgggcgtaag gccggaacag gctgaggcgt tttatcatcg cctgacccag
    17761 tgcaaaaacg ttcgtcagcc ggtgaatatc gtcagccatt ttgcgcgcgc ggatgaacca
    17821 aaatgtggcg caaccgagaa acaactcgct atctttaata ccttttgcga aggcaaacct
    17881 ggtcaacgtt ccattgccgc gtcgggtggc attctgctgt ggccacagtc gcattttgac
    17941 tgggtgcgcc cgggcatcat tctttatggc gtctcgccgc tggaagatcg ctccaccggt
    18001 gccgattttg gctgtcagcc agtgatgtca ctaacctcca gcctgattgc cgtgcgtgag
    18061 cataaagccg gagagcctgt tggttatggt ggaacctggg taagcgaacg tgatacccgt
    18121 cttggcgtag tcgcgatggg ctatggcgat ggttatccgc gcgccgcgcc gtccggtacg
    18181 ccagtgctgg tgaacggtcg cgaagtaccg attgtcgggc gcgtggcgat ggatatgatc
    18241 tgcgtagact taggtccaca ggcgcaggac aaagccgggg atccggtcat tttatggggc
    18301 gaaggtttgc ccgtagaacg tatcgctgaa atgacgaaag taagcgctta cgaacttatt
    18361 acgcgcctga cttcaagggg cgcgatgaaa tacgtggatt aatcgttctg taatatttga
    18421 ttgtctgtgc cggatgcggc gtgaatgcct tatccggcca ataaaatcct aaaaattcaa
    18481 taagttgatg ttctttcatg ctcttataaa ggtcgtgcct ctggcggatg tacgtttatc
    18541 attagtctca ctctgttgct aattgccgtt cgctcctgaa catccactcg atcttcgcct
    18601 tcttccggtt tattgtgttt taaccacctg cccgtaaacc tggagaacca tcgcgtgttt
    18661 caaaaagttg acgcctacgc tggcgacccg attcttacgc ttatggagcg ttttaaagaa
    18721 gaccctcgca gcgacaaagt gaatttaagt atcggtctgt actacaacga agacggaatt
    18781 attccacaat tgaaagccgt ggcggaggcg gaagcgcgcc tgaatgcgca gccccatggc
    18841 gcttcgcttt atttaccgat ggaagggctt aacagctatc gccatgccat tgcgccgctg
    18901 ctgtttggtg cggaccatcc ggtactgcaa caacagcgcg tagcaaccat tcaaaccctt
    18961 ggcggctcag gggcattgaa agtgggcgcg gatttcctga aacgctactt cccggaatca
    19021 ggcgtctggg tcagcgatcc tacttgggaa aaccacgtag caatattcgc cggggctgga
    19081 ttcgaagtga gtacttaccc ctggtatgac gaagtgacta acagcgtgcg ctttaatgac
    19141 ctgttggcga cgctgaaaac attacctgcc cgcagtattg tgttgctgca tccatgttgc
    19201 cacaacccaa cgggtgccga tctcactaat gatcagtggg atgcggtgat tgaaattctc
    19261 aaagcccgcg agcttatccc attcctcgat attgcctatc aaggatttag tgccggtatg
    19321 gaagaggatg cctacgctat tcgcgccatt gccagcgctg gattacccgc tctggtgagc
    19381 aattcgttct cgaaaatttt ctccctttac ggcgagcgcg tcggcggact ttctgttctg
    19441 tgtgaagatg ccgaagccgc tggccgcgta ctggggcaat tgaaagcaac agttcgccgc
    19501 aactactcca gcccgccgaa ttttggtgcg caggtggtgg ctgcagtgct gaatgacgag
    19561 gcattgaaag ccagctggct ggcggaagta gaagagatgc gtactcgcat tctggcaatg
    19621 cgtcaggaat tggtgaaggt attaagcaca gagatgccag aacgcaattt cgattatctg
    19681 cttaatcagc gcggcatgtt cagttatacc ggtttaagtg ccgctcaggt tgaccgacta
    19741 cgtgaagaat ttggtgtcta tctcatcgcc agcggtcgca tgtgtgtcgc cgggttaaat
    19801 acggcaaatg tacaacgtgt ggcaaaggcg tttgctgcgg tgatgtaatg caggaaagca
    19861 ggctggagtt acccagcctg cagtgaaatt aaactgtcgt cgctttcact atttctttgt
    19921 agatgatttt tttgatgccg ttgttctacg tgagagataa taaaacgttg tcagttcttt
    19981 tattgttaag tttatcccaa ttatctggaa ttcctttccc tattttttcg tgtggagtaa
    20041 taatttctgc acatctaacc attattaaat ttaataataa gtatcgggtt tgttacgata
    20101 gaatatttct atatcctcaa gaatatgctt tgccttattg cctgtgactt tctgagattc
    20161 aattatagta tcgttaaatt ctaatgttaa agagaactct ttttttccgc tttgtgtaag
    20221 tttaacctta tattgattac caatatcaaa taaaaaggtc ctgtaatgag aataatcacc
    20281 tttaacctcc tcggcaaaac agcaaagcgt gcaaatatac ataaagtctt tcttacccct
    20341 gattttgtta actaaatcaa taaatgcgtg aaaaacttta cttgcaatag aacttgatac
    20401 ttcttctcta ggtctacaaa aatcttttgc aatttttaac tgtgtttcta tgtataaaaa
    20461 gaaatctttg gcataagaga aatctttggc ataagagata tctttattat ttagtgcatc
    20521 tagctcaaca ggtaaagatg tcgttttgtt gctattcaca tatgatatat tcatatcatc
    20581 atctccatcg cgagtaataa aattaatcac cattgtaggg tagggggctg gtcaatcaga
    20641 aatcatcttt ataaacttcg attgtttttg taatgctgta tcattaagtt cattaaatcg
    20701 tacagcagat aaatgttcta tcaaatttcg ctcatttgcc gaggattcat cataataaac
    20761 gtaaaattaa tgtatcctta catcgagtaa taaacatttt ttatacaaaa aaagacagga
    20821 acgtatttac tgggttaaat ataatcatcc tgcttttcat cacaaaaacc gcagataatc
    20881 cttcctttcc ccggcagctg gcgttatggt cagatggttt ttgcaacaaa tctcacaata
    20941 aaaagtttca acatactgac tatttaggga aaaatatgcg caagatcaca caggcaatca
    21001 gtgccgtttg cttattgttc gctctaaaca gttccgctgt tgccctggcc tcatctcctt
    21061 caccgcttaa ccctgggact aacgttgcca ggcttgctga acaggcacca attcattggg
    21121 tttcggtcgc acaaattgaa aatagcctcg cagggcgtcc gccaatggcg gtggggtttg
    21181 atatcgatga cacggtactt ttttccagtc cggggttctg gcgcggcaaa aaaaccttct
    21241 cgccagaaag cgaagattat ctgaaaaatc ctgtgttctg ggaaaaaatg aataatggct
    21301 gggatgaatt cagcattcca aaagaggtcg ctcgccagct gattgatatg catgtacgcc
    21361 gcggtgacgc gatcttcttt gtgactggtc gtagcccgac gaaaacagaa acggtttcaa
    21421 aaacgctggc ggataatttt catattcctg ccaccaacat gaatccggta atctttgcgg
    21481 gcgataaacc agggcaaaat acaaaatcgc aatggctgca ggataaaaat atccgaattt
    21541 tttatggcga ttccgataac gatattaccg ccgcacgcga tgtcggcgct cgtggtatcc
    21601 gcattctgcg cgcctccaac tctacctaca aacccttgcc acaagcgggt gcgtttggtg
    21661 aagaggtgat cgtcaattca gaatactgac agagcgggag agagtgatgc tctcccgaat
    21721 gctgtttttt taatcacacc tttatccttt cgctgtcttg ctgcaaactg attaagagag
    21781 ttttatcaag gagcagcaca tgtggtatca aaagacgctc acgcttagcg ccaaatctcg
    21841 tgggtttcat ctggtaacgg atgaaattct gaatcagctg gctgatatgc cgcgcgttaa
    21901 catcggctta ctgcatctgt tgctgcaaca tacctccgcc tctctgacac ttaatgagaa
    21961 ctgcgatccc accgtacgcc acgacatgga gtgttttttc ctccgcaccg ttcccgacaa
    22021 cggaaattat gagcatgact atgagggagc agacgatatg ccttctcata tcaaatcctc
    22081 aatgctggga acatcgcttg tattgccggt acataaaggg cgtattcaga ccggcacttg
    22141 gcaaggcatc tggctgggag aacatcgcat ccacggcgga tcgcgtcgca tcatcgcgac
    22201 actacaaggg gagtaaaaaa tgaccatttc ggagttgcta caatattgca tggcaaaacc
    22261 aggcgcagaa cagagcgtgc ataacgactg gaaagcgacg cagatcaaag tggaagatgt
    22321 actgtttgcg atggtgaaag aagtagaaaa tcgcccagct gtttcgctga aaaccagccc
    22381 ggagctggcg gagctgctac gtcagcagca cagcgatgtg cgtcctagcc gccatctgaa
    22441 taaagcgcac tggagcaccg tgtatctcga cggttcgctg ccggattcgc aaatctatta
    22501 tctggtggat gcgtcttatc agcaggcagt gaatttacta ccggaagaaa agcgtaaatt
    22561 gctggtacaa ctctgaaagg aaaaggccgc tcagaaagcg gccttaacga ttacagcatc
    22621 ggcttgagga agcgtgccgt atgcgaagct tcgcactccg cgacggtttc tggcgtaccg
    22681 gagacgagga tttcgccgcc gccactgccg ccttctggtc ccaggtcgac aatccagtca
    22741 gcggttttga tcacgtcgag attgtgctca atcaccacaa tggtattgcc ctgatcgcgc
    22801 agtttatgca gcacgtcgag gagttgctga atatcggcga agtgcagacc gctggtcggc
    22861 tcgtcgagaa tatacagcgt ctgcccggtg ccgcgttttg acagctcacg cgccagcttc
    22921 acgcgctggg cttcaccacc agaaagtgtg gttgcggact gccccaggcg aatgtacgtc
    22981 agaccaacgt ccatcaacgt ttgcagctta cgcgccaggg ctggcaccgc atcaaagaac
    23041 tcacgcgcct cttcgatggt catatccagc acttcgtgga tggttttgcc tttgtactta
    23101 atttccagcg tttcacggtt atagcgttta cctttgcact gatcgcacgg cacgtagata
    23161 tccggcagga agtgcatctc cactttgatc acaccgtcgc cctgacaggc ttcgcagcgc
    23221 ccgccacgga cgttaaagct gaaacgtcct ggcgtataac cacgcgcacg ggattccggt
    23281 acgcccgcaa acagttcgcg cacaggcgta aacacgccgg tataggtcgc cgggttagaa
    23341 cgtggagtac gaccaattgg gctttggtcg atatcgatca ctttatcgaa atgctccagt
    23401 ccctgaatat cgcgatacgg tgccggttcg gcgatggtcg caccattcaa ctggcgttgg
    23461 gcaatcggga acagtgtgtc gttaatcagc gtcgatttac cggaacctga aacccctgtg
    23521 atgcaggtaa acagaccgac tggcagcgtg agcgtcacgt ccttcaggtt gttgccgcgt
    23581 gcgcctgtca gcttcagcac tttttctgga ttcgccggaa cgcgtttctt cggcacttca
    23641 atcttgcgtt taccgctcat gtactgcccg gtcaacgatt caggcaccgc cataatcgct
    23701 tccagcggac cttctgcgac cacttcaccg ccgtgaacac ccgcaccagg accgatatcg
    23761 atcacatggt cagcggcgcg aattgcgtct tcgtcatgct ccaccacaat cacggtatta
    23821 ccgagatcgc gcagatggat aagcgtaccc aacaggcgct cgttatcgcg ctggtgcagg
    23881 ccgatagacg gctcgtccag cacgtacata acgccaacca ggcccgcacc aatctggctc
    23941 gccagacgga tacgctgggc ttcaccgccg gaaagtgttt ctgccgagcg ggaaagtgtc
    24001 aggtaattca ggccgacgtt tacgaggaat ttcaggcgat cgccgatctc tttaagaatt
    24061 ttttccgcaa tcttcgcccg ctgaccagcg agtttgagat tgttgaagaa ttccatcgca
    24121 tgaccgatgc tcatgtcgga gatagcaggc agcggcgtat tctcgacata aacgtggcgc
    24181 gcttcccgac gcagacgcgt cccttcgcag ctggcgcatg ggcgattgct gataaacttg
    24241 gctaattctt cacgtaccgc actggattct gtctctttat aacggcgctc catattgtgc
    24301 agcacgcctt cgaacggatg acgacggatg gaggtatcgc cacgatcgtt catgtatttg
    24361 aattcaatgt tttctttgcc agaaccgtac aacaccactt tatgcacgtt cgcgctcagg
    24421 ctgccccacg gcgcttcgac gtcgaactta tagtgatctg ccagcgattt cagcatctgg
    24481 aagtaataga agttgcggcg atcccagcca cggatcgcac cgccagccag tgacagctcg
    24541 gggttttgga tcacgcggtc aggatcgaaa tattgctgta cgccaaggcc gtcacaggtc
    24601 gggcaggcac ctgccgggtt gttaaacgaa aacagtcgcg gctccagttc acgcatactg
    24661 tagccgcaaa ttgggcaggc gaagttggcg gagaacagca gctcttccgc tttcgggtcg
    24721 tccatatccg ccactaccgc tgtaccaccg gaaagttcca gcgcggtttc aaacgactcc
    24781 gcaagacgtt gggtaagatc gtcacgcacc ttgaagcgat caaccaccac ttcaatggta
    24841 tgtttctttt gcagttccag tttcggcgga tcggaaagat cgcagacttc gccatcaata
    24901 cgagcacgga tgtaaccctg gcttgccagg ttctccagcg ttttggtgtg ttcgcctttg
    24961 cgctctttaa tgattggcgc aagcagcatc agacgcttgc cttccggctg cgacagcacg
    25021 ttatccacca tctggctgac ggtttgcgcc gccagcggga cgtcgtggtc cggacagcgc
    25081 ggttcgccga cgcgggcgaa caacaaacgc aaatagtcgt ggatttcggt gattgtcccc
    25141 accgtagaac gcgggttatg agacgtcgat ttctgctcaa ttgagatggc aggagaaagc
    25201 ccctcaatat gatcgacgtc cggcttttcc atcagtgaca gaaactgccg cgcgtaggcg
    25261 gaaagggatt caacgtaacg gcgctgccct tcggcatata aggtgtcgaa agcgagcgag
    25321 gatttgccag aacccgaaag cccggtcacg acaatgagct tgtcgcgggg aataacgagg
    25381 ttgatgtttt tgagattatg ggtgcgggcg ccccgaactt cgatcttatc cattcacctt
    25441 tcccggatta aacgcttttt tgcccggtgg catggtgcta ccggcgatca caaacggtta
    25501 attatgacac aaattgacct gaatgaatat acagtattgg aatgcattat ccggagtgtt
    25561 gtgtaacaat gtctggccag gtttgtttcc cggaaccgag gtcacaacat agtaaaagcg
    25621 ctattggtaa tggtacaatc gcgcgtttac acttattcag aacgattttt ttcaggagac
    25681 acgaacatgg ccagcagagg cgtaaacaag gttattctcg ttggtaatct gggtcaggac
    25741 ccggaagtac gctacatgcc aaatggtggc gcagttgcca acattacgct ggctacttcc
    25801 gaatcctggc gtgataaagc gaccggcgag atgaaagaac agactgaatg gcaccgcgtt
    25861 gtgctgttcg gcaaactggc agaagtggcg agcgaatatc tgcgtaaagg ttctcaggtt
    25921 tatatcgaag gtcagctgcg tacccgtaaa tggaccgatc aatccggtca ggatcgctac
    25981 accacagaag tcgtggtgaa cgttggcggc accatgcaga tgctgggtgg tcgtcagggt
    26041 ggtggcgctc cggcaggtgg caatatcggt ggtggtcagc cgcagggcgg ttggggtcag
    26101 cctcagcagc cgcagggtgg caatcagttc agcggcggcg cgcagtctcg cccgcagcag
    26161 tccgctccgg cagcgccgtc taacgagccg ccgatggact ttgatgatga cattccgttc
    26221 tgatttgtca ttaaaacaat aggttatatt gttttaaggt ggatgattaa agcatctgcc
    26281 agccataaaa aagaagcctc cgttatggag g
//
LOCUS       DADLSB010000042        26151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.042, whole genome shotgun sequence.
ACCESSION   DADLSB010000042 DADLSB010000000
VERSION     DADLSB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.042"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            214..408
                     /gene="bfd"
                     /locus_tag="JN331_001720"
     CDS             214..408
                     /gene="bfd"
                     /locus_tag="JN331_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY7259886.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            481..957
                     /gene="bfr"
                     /locus_tag="JN331_001721"
     CDS             481..957
                     /gene="bfr"
                     /locus_tag="JN331_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY7259887.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(954..1421)
                     /locus_tag="JN331_001722"
     CDS             complement(954..1421)
                     /locus_tag="JN331_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY7259888.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1800..2111
                     /gene="rpsJ"
                     /locus_tag="JN331_001723"
     CDS             1800..2111
                     /gene="rpsJ"
                     /locus_tag="JN331_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY7259889.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2144..2773
                     /gene="rplC"
                     /locus_tag="JN331_001724"
     CDS             2144..2773
                     /gene="rplC"
                     /locus_tag="JN331_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY7259890.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2784..3389
                     /gene="rplD"
                     /locus_tag="JN331_001725"
     CDS             2784..3389
                     /gene="rplD"
                     /locus_tag="JN331_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY7259891.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3386..3688
                     /gene="rplW"
                     /locus_tag="JN331_001726"
     CDS             3386..3688
                     /gene="rplW"
                     /locus_tag="JN331_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY7259892.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3706..4527
                     /gene="rplB"
                     /locus_tag="JN331_001727"
     CDS             3706..4527
                     /gene="rplB"
                     /locus_tag="JN331_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY7259893.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKLGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4544..4822
                     /gene="rpsS"
                     /locus_tag="JN331_001728"
     CDS             4544..4822
                     /gene="rpsS"
                     /locus_tag="JN331_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY7259894.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4837..5169
                     /gene="rplV"
                     /locus_tag="JN331_001729"
     CDS             4837..5169
                     /gene="rplV"
                     /locus_tag="JN331_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY7259895.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5187..5888
                     /gene="rpsC"
                     /locus_tag="JN331_001730"
     CDS             5187..5888
                     /gene="rpsC"
                     /locus_tag="JN331_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY7259896.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5901..6311
                     /gene="rplP"
                     /locus_tag="JN331_001731"
     CDS             5901..6311
                     /gene="rplP"
                     /locus_tag="JN331_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY7259897.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6311..6502
                     /gene="rpmC"
                     /locus_tag="JN331_001732"
     CDS             6311..6502
                     /gene="rpmC"
                     /locus_tag="JN331_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY7259898.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6502..6756
                     /gene="rpsQ"
                     /locus_tag="JN331_001733"
     CDS             6502..6756
                     /gene="rpsQ"
                     /locus_tag="JN331_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY7259899.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6921..7292
                     /gene="rplN"
                     /locus_tag="JN331_001734"
     CDS             6921..7292
                     /gene="rplN"
                     /locus_tag="JN331_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY7259900.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7303..7617
                     /gene="rplX"
                     /locus_tag="JN331_001735"
     CDS             7303..7617
                     /gene="rplX"
                     /locus_tag="JN331_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY7259901.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7632..8171
                     /gene="rplE"
                     /locus_tag="JN331_001736"
     CDS             7632..8171
                     /gene="rplE"
                     /locus_tag="JN331_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY7259902.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8186..8491
                     /gene="rpsN"
                     /locus_tag="JN331_001737"
     CDS             8186..8491
                     /gene="rpsN"
                     /locus_tag="JN331_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY7259903.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8525..8917
                     /gene="rpsH"
                     /locus_tag="JN331_001738"
     CDS             8525..8917
                     /gene="rpsH"
                     /locus_tag="JN331_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY7259904.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8930..9463
                     /gene="rplF"
                     /locus_tag="JN331_001739"
     CDS             8930..9463
                     /gene="rplF"
                     /locus_tag="JN331_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY7259905.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9473..9826
                     /gene="rplR"
                     /locus_tag="JN331_001740"
     CDS             9473..9826
                     /gene="rplR"
                     /locus_tag="JN331_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY7259906.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9841..10344
                     /gene="rpsE"
                     /locus_tag="JN331_001741"
     CDS             9841..10344
                     /gene="rpsE"
                     /locus_tag="JN331_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY7259907.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10348..10527
                     /gene="rpmD"
                     /locus_tag="JN331_001742"
     CDS             10348..10527
                     /gene="rpmD"
                     /locus_tag="JN331_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY7259908.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10531..10965
                     /gene="rplO"
                     /locus_tag="JN331_001743"
     CDS             10531..10965
                     /gene="rplO"
                     /locus_tag="JN331_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY7259909.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10973..12304
                     /gene="secY"
                     /locus_tag="JN331_001744"
     CDS             10973..12304
                     /gene="secY"
                     /locus_tag="JN331_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY7259910.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12336..12452
                     /gene="rpmJ"
                     /locus_tag="JN331_001745"
     CDS             12336..12452
                     /gene="rpmJ"
                     /locus_tag="JN331_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY7259911.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12599..12955
                     /gene="rpsM"
                     /locus_tag="JN331_001746"
     CDS             12599..12955
                     /gene="rpsM"
                     /locus_tag="JN331_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY7259912.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12972..13361
                     /gene="rpsK"
                     /locus_tag="JN331_001747"
     CDS             12972..13361
                     /gene="rpsK"
                     /locus_tag="JN331_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY7259913.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13395..14015
                     /gene="rpsD"
                     /locus_tag="JN331_001748"
     CDS             13395..14015
                     /gene="rpsD"
                     /locus_tag="JN331_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY7259914.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14041..15030
                     /gene="rpoA"
                     /locus_tag="JN331_001749"
     CDS             14041..15030
                     /gene="rpoA"
                     /locus_tag="JN331_001749"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY7259915.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15071..15454
                     /gene="rplQ"
                     /locus_tag="JN331_001750"
     CDS             15071..15454
                     /gene="rplQ"
                     /locus_tag="JN331_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY7259916.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15561..15929
                     /locus_tag="JN331_001751"
     CDS             15561..15929
                     /locus_tag="JN331_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HAY7259917.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15940..16365
                     /gene="zntR"
                     /locus_tag="JN331_001752"
     CDS             15940..16365
                     /gene="zntR"
                     /locus_tag="JN331_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HAY7259918.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16421..16639
                     /locus_tag="JN331_001753"
     CDS             16421..16639
                     /locus_tag="JN331_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HAY7259919.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16636..17046)
                     /gene="mscL"
                     /locus_tag="JN331_001754"
     CDS             complement(16636..17046)
                     /gene="mscL"
                     /locus_tag="JN331_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAY7259920.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGGIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17176..18552)
                     /gene="trkA"
                     /locus_tag="JN331_001755"
     CDS             complement(17176..18552)
                     /gene="trkA"
                     /locus_tag="JN331_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY7259921.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18574..19863)
                     /gene="rsmB"
                     /locus_tag="JN331_001756"
     CDS             complement(18574..19863)
                     /gene="rsmB"
                     /locus_tag="JN331_001756"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY7259922.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(19909..20856)
                     /gene="fmt"
                     /locus_tag="JN331_001757"
     CDS             complement(19909..20856)
                     /gene="fmt"
                     /locus_tag="JN331_001757"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY7259923.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20871..21380)
                     /gene="def"
                     /locus_tag="JN331_001758"
     CDS             complement(20871..21380)
                     /gene="def"
                     /locus_tag="JN331_001758"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY7259924.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21511..22635
                     /gene="dprA"
                     /locus_tag="JN331_001759"
     CDS             21511..22635
                     /gene="dprA"
                     /locus_tag="JN331_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY7259925.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGLLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22607..23080
                     /locus_tag="JN331_001760"
     CDS             22607..23080
                     /locus_tag="JN331_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY7259926.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23109..23651
                     /locus_tag="JN331_001761"
     CDS             23109..23651
                     /locus_tag="JN331_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY7259927.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23656..24228
                     /gene="tsaC"
                     /locus_tag="JN331_001762"
     CDS             23656..24228
                     /gene="tsaC"
                     /locus_tag="JN331_001762"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY7259928.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24233..25051
                     /gene="aroE"
                     /locus_tag="JN331_001763"
     CDS             24233..25051
                     /gene="aroE"
                     /locus_tag="JN331_001763"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY7259929.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEEVAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25048..25305
                     /locus_tag="JN331_001764"
     CDS             25048..25305
                     /locus_tag="JN331_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY7259930.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25281..25835)
                     /locus_tag="JN331_001765"
     CDS             complement(25281..25835)
                     /locus_tag="JN331_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY7259931.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6720 a         6195 c         6783 g         6453 t
ORIGIN      
        1 cgcgggcgtt gttgctaaag ttctgggcta attacacgtt aattagtttt gaattgaaaa
       61 gggcgcttcg gcgccctttt tgctgcccgt aatttgagct cgaaatattt agtcgtaatt
      121 ttgctcgctg aactatggcg caaataaaac tgtgctattg taatcataac cattctcatt
      181 tacactttgc gcagaatttg aacgggagca atcatgtacg tttgtttgtg taatggtgta
      241 agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc cgcaatcttt ccaacaattg
      301 cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca ttcgcgccgc gcgcgaagtg
      361 atgcaggacg agttgatgca gatgccagaa tttaaagaga ttgcctgaag cccacgctta
      421 ttttttgaca tcccgctagc cccatctacg ctctaatagt ggaagcggag ggactataaa
      481 atgaaaggtg atgttaaaat cataaattat ctcaataaac tattgggaaa tgagcttgtc
      541 gcaatcaatc agtattttct ccatgcccgg atgtttaaaa actggggcct gatgcgtctc
      601 aatgacgtcg aataccatga atccatcgat gagatgaaac acgctgataa atatatcgag
      661 cgtattttgt ttttagaagg aatccccaac ttacaggatt taggcaaact ggggattggt
      721 gaagatgtcg aggagatgct gcaatctgac ctgcgattag aactggaagg cgcgaaggat
      781 ctgcgtgagg ctatcgcata tgccgacagc gttcatgact atgttagccg cgatatgatg
      841 atcgagatcc ttgctgatga agaaggtcat atcgactggc tcgaaactga actggatctc
      901 atcggtaaaa tcggcctaca aaattacctg caatcacaaa ttaaagttaa agattaacgg
      961 cctatcagca aactatccca gcctataacg aaacccgcag ccgccagaaa tggcccaaaa
     1021 ggcagcgggt tttttaatga ttcttttcct ctcatgagca gaccaataac tacggctccg
     1081 caagcgaatg aggcagcaag gaaaaccagt cgtggtaaga aggcccatga atgccaggcg
     1141 ccaagggcgg cgagaaactt cacatcacca tagcctaatc cttctttatg tcgcagtatg
     1201 cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa caattgcccc ccataacgca
     1261 tcggctaaac catcaggatg acaaacttga tagaacaaaa gacctgacca gagtaacggg
     1321 cacgtaaatc tgtcaggtaa caagccatgc tttgcatccc agaaaaaaag tagggctgat
     1381 aaacaagcgt acaaaattag aaagggaagg gtagtgtcca taaaacatcc tctgtaattg
     1441 gagtatcacc atcgaataac tccggatgct cagcaacaga gagagcgctg atttgttaac
     1501 ctgattccgg tgaaaaacgc tatcttcaca accttacaaa tttcgtggaa tgcagctcgc
     1561 aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt caggctcaga tttgcgtata
     1621 atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga acctcagtta gccgaatttg
     1681 gctacctaac aatgctccca atcggggagc tacgtaagaa cggttacact ctcccatcaa
     1741 tcgtaatggg tctgaggagt aatcattttc gtttataaaa taattggagc tctggtctca
     1801 tgcagaacca aagaatccgt atccgcctga aagcgtttga tcatcgtctg atcgatcaag
     1861 caaccgcgga aatcgtcgag actgccaagc gcactggtgc gcaggtccgt ggtccgatcc
     1921 cgctgccgac acgcaaagag cgcttcactg ttctgatctc cccgcacgtc aacaaagacg
     1981 cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt tgacatcgtt gagccaaccg
     2041 agaaaaccgt tgatgctctg atgcgtctgg atctggctgc cggtgtagac gtgcagatca
     2101 gcctgggtta atcaggtcat tgagcgattg agaggttgaa acaatgattg gtttagtcgg
     2161 taaaaaagtg ggtatgaccc gtatcttcac agaagacggc gtttctatcc cagtaaccgt
     2221 aatcgaagtt gaagcaaacc gcgttactca ggttaaagac ctggctaacg atggctaccg
     2281 tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt gtgaccaagc ctgaagctgg
     2341 ccacttcgct aaagctggcg tagaagctgg ccgtggtctg tgggaattcc gcctggctga
     2401 aggcgaagag ttcactgtag gtcagagcat tagcgttgaa ctgtttgctg acgttaaaaa
     2461 agttgacgta actggcacct ctaaaggtaa aggtttcgca ggtaccgtta agcgctggaa
     2521 cttccgtacc caggacgcta ctcacggtaa ctccttgtct caccgcgttc cgggttctat
     2581 cggtcagaac cagactccgg gcaaagtgtt caaaggcaag aaaatggcag gtcagatggg
     2641 taacgaacgt gtaaccgttc agagccttga cgtagtacgc gttgacgctg agcgcaacct
     2701 gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc gacctgatcg ttaaaccagc
     2761 tgtgaaggcg taaggagata gcaatggaat tagtattgaa agacgcgcag agcgcgctga
     2821 ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc gctggttcac caggttgttg
     2881 ttgcttatgc agctggtgct cgtcagggta ctcgtgctca gaagactcgt gctgaagtaa
     2941 ctggttccgg taaaaaaccg tggcgccaga aaggcaccgg ccgtgcgcgt tctggttcta
     3001 tcaagagccc gatctggcgt tctggtggcg tgacctttgc tgctcgtccg caggaccaca
     3061 gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa aagcatcctg tccgaactgg
     3121 tacgtcagga tcgtctgatc gttgtcgaga agttctctgt agaagcgccg aaaactaagc
     3181 tgctggcaca gaaactgaaa gacatggctc tggaagatgt gctgatcatc accggtgagc
     3241 tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa ggttgacgta cgcgatgcaa
     3301 ctggtatcga cccggttagc ctgatcgcct tcgacaaagt cgtaatgact gctgatgctg
     3361 ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa cgtctgctga aggtgctgcg
     3421 tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa aaatccaaca ccatcgtact
     3481 caaagttgcc aaagacgcga ccaaagcaga aatcaaagct gctgtgcaga aactgtttga
     3541 agtcgaagtc gaagtcgtta acaccctggt agttaaaggg aaagttaaac gtcacggaca
     3601 gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc accctgaaag aaggccagaa
     3661 tctggacttc gttggcggcg ctgagtaagt cggaggagta atacaatggc agttgttaaa
     3721 tgtaaaccga catctccggg tcgtcgccac gtagttaaag tggttaaccc tgagctgcac
     3781 aagggcaaac cttttgctcc gttgctggaa aaaaacagca aatccggtgg tcgtaacaac
     3841 aatggccgta tcaccactcg tcatatcggt ggtggccaca agcaggctta ccgtattgtt
     3901 gacttcaaac gcaacaaaga cggtatcccg gcagttgttg aacgtcttga gtacgatccg
     3961 aaccgttccg cgaacatcgc gctggttctg tacaaagacg gtgaacgccg ttacatcctg
     4021 gcccctaaag gcctgaaagc tggcgaccag attcagtctg gcgttgatgc tgcaatcaaa
     4081 ctaggtaaca ccctgccgat gcgcaacatc ccggttggtt ctactgttca taacgtagaa
     4141 atgaaaccag gtaaaggcgg tcagctggca cgttccgctg gtacttacgt tcagatcgtt
     4201 gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg gtgaaatgcg taaagtagaa
     4261 gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg agcatatgct gcgcgttctg
     4321 ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga ccgttcgcgg taccgcgatg
     4381 aacccggtag accacccaca tggtggtggt gaaggtcgta actttggtaa gcacccggta
     4441 actccgtggg gcgttcagac caaaggtaag aagacccgca gcaacaagcg tactgataaa
     4501 ttcatcgtac gtcgccgtag caaataattt tagaggataa gccatgccac gttctctcaa
     4561 gaaaggtcct tttattgacc tgcacttgct gaagaaggta gagaaagcgg tggaaagcgg
     4621 agacaagaag cccctgcgca cttggtcccg tcgttcaacg atctttccta acatgatcgg
     4681 tttgaccatc gctgtccata atggtcgtca gcacgttccg gtatttgtaa ccgacgaaat
     4741 ggtcggtcac aaactgggtg aattcgcacc gactcgtact tatcgcggcc acgctgctga
     4801 taaaaaagcg aagaagaaat aaggtaggag gaagagatgg aaactatcgc taaacatcgc
     4861 catgctcgtt cttctgctca gaaggttcgc cttgttgctg acctgattcg cggtaagaaa
     4921 gtgtcgcagg ctctggatat tttgacctac accaacaaga aagcggctgt actggtcaag
     4981 aaagttctgg aatctgccat tgctaacgct gaacacaacg atggcgctga cattgacgat
     5041 ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca tgaagcgcat tatgccgcgt
     5101 gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc acatcactgt ggttgtgtcc
     5161 gatcgctgag actctggaga ctagcaatgg gtcagaaagt acatcctaat ggtattcgcc
     5221 tgggtattgt aaaaccatgg aactctacct ggtttgcgaa caccaaagaa ttcgctgaca
     5281 acctggacag cgattttaaa gtacgtcagt acctgactaa ggaactggct aaagcgtccg
     5341 tatctcgtat cgttatcgag cgtccggcta agagcatccg tgtaaccatt cacactgctc
     5401 gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga aaaactgcgt aaggtcgtag
     5461 cggacatcgc tggcgttcct gcacagatca acatcgccga agttcgtaag cctgaactgg
     5521 acgcaaaact ggttgctgac agcatcactt ctcagctgga acgtcgcgtt atgttccgtc
     5581 gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg cgctaaaggt attaaagttg
     5641 aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac cgaatggtac cgcgaaggtc
     5701 gcgtaccgct gcacactctg cgtgctgaca tcgactacaa cacctctgaa gcgcacacca
     5761 cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg cgagatcctg ggtggtatgg
     5821 ctgctgttga acaaccggaa aaaccggctg ctcagcctaa aaagcagcag cgtaaaggcc
     5881 gtaaataagg agcgtcgctg atgttacaac caaagcgtac aaaattccgt aaaatgcaca
     5941 aaggccgtaa ccgcggtctg gcgcagggta cggatgttag cttcggcagc ttcggtctga
     6001 aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga agcagcacgt cgtgctatga
     6061 cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt gttcccggac aaaccgatca
     6121 ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg taacgtggag tattgggttg
     6181 ccttgattca gccgggtaaa gtcctgtatg aaatggacgg tgttccggaa gagctggccc
     6241 gtgaagcatt caagctggca gcagcgaaac tgccgattaa aaccaccttt gtaactaaga
     6301 cggtgatgta atgaaagcaa aagagctgcg tgagaagagc attgaagagc tgaacaccga
     6361 gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg caggctgcaa gtggccagct
     6421 gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc gcacgcgtta agactttact
     6481 gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta ctctgcaagg tcgcgttgtt
     6541 agcgacaaaa tggagaaatc cattgttgtt gctatcgaac gttttgtgaa acacccgatc
     6601 tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac atgacgagaa caacgaatgc
     6661 ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc tgtccaagac taaatcctgg
     6721 acgctggttc gcgttgtaga gaaagctgtt ctgtaataca gtacactctc tcgatacgaa
     6781 taaacggctc agaaatgagc cgtttatttt ttctacccat atccttgaag cggtgttata
     6841 atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt ttcgggtctc agtagtagtt
     6901 gacattagcg gagcactaaa atgatccaag aacagactat gctgaacgtc gccgacaact
     6961 ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg ctcgcaccgt cgctacgcag
     7021 gcgtaggcga catcatcaag atcaccatca aagaagcaat tccgcgtggt aaggtcaaaa
     7081 aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa gggtgttcgt cgcccggacg
     7141 gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct gaacaacaac agcgagcagc
     7201 ctatcggtac gcgtattttt gggccggtaa ctcgtgagct tcgtagtgag aagttcatga
     7261 aaattatctc tctggcacca gaagtactct aaggagcgaa tcatggcagc gaaaatccgt
     7321 cgtgatgacg aagttatcgt gttaaccggt aaagataaag gtaaacgcgg taaagttaag
     7381 aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca acctggttaa gaaacatcag
     7441 aagccggttc cggccctgaa ccaaccgggt ggcatcgttg aaaaagaagc cgctattcag
     7501 gtttccaacg tagcaatctt caatgcggca accggcaagg ctgaccgtgt aggctttaga
     7561 ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca gcgaaactat caagtaattt
     7621 ggagtagtac gatggcgaaa ctgcatgatt actacaaaga cgaagtagtt aaaaaactca
     7681 tgactgagtt taactacaat tctgtcatgc aagtccctcg ggtcgagaag atcaccctga
     7741 acatgggtgt tggtgaagcg atcgctgaca aaaaactgct ggataacgca gcagcagacc
     7801 tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc acgcaaatct gttgcaggct
     7861 tcaaaatccg tcagggctat ccgatcggct gtaaagtaac tctgcgtggc gaacgcatgt
     7921 gggagttctt tgagcgcctg atcactattg ctgtacctcg tatccgtgac ttccgtggcc
     7981 tgtccgctaa gtctttcgac ggtcgtggta actacagcat gggtgtccgt gagcagatca
     8041 tcttcccaga aatcgactac gataaagtcg accgcgttcg tggtttggat attaccatta
     8101 ccactactgc gaaatctgac gaagaaggcc gcgctctgct ggctgccttt gacttcccgt
     8161 tccgcaagta aggtagggtt actaaatggc taagcaatca atgaaagcac gcgaagtaaa
     8221 acgcgtagct ttagctgata aatacttcgc gaaacgcgct gaactgaaag cgatcatctc
     8281 tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt ctcaagctgc agactctgcc
     8341 gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt caaacaggtc gtccgcatgg
     8401 tttcctgcgg aagttcgggt tgagccgtat taaggtccgt gaagccgcta tgcgcggtga
     8461 aatcccgggt ctgaaaaagg ctagctggta attgtcacca attgaatcac gggagtaaag
     8521 acagatgagc atgcaagatc cgatcgcgga tatgctgacc cgtatccgta acggtcaggc
     8581 cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg aaagtggcaa tcgccaacgt
     8641 gctgaaggaa gaaggtttta ttgaagattt taaagttgaa ggcgacacca agcctgaact
     8701 ggaacttact ctgaagtatt tccagggcaa agctgttgta gaaagcattc agcgtgtcag
     8761 ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg ccgaaagtta tggcgggtct
     8821 gggtatcgca gttgtttcta cctctaaagg tgttatgact gatcgtgcag cgcgccaggc
     8881 tggtcttggt ggcgaaatta tctgctacgt agcctaatcg gaggaaaaaa tgtctcgtgt
     8941 tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta aaaatcaacg gtcaggttat
     9001 tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc aacgatgctg ttgaagttaa
     9061 acatgcagat aataccctga ccttcggtcc gcgtgatggt tacgcagacg gttgggcaca
     9121 ggctggtacc gcgcgtgccc tgctgaactc aatggttatc ggtgttaccg aaggcttcac
     9181 taagaagctg cagctggttg gtgtaggtta ccgtgcagcg gttaaaggca atgtgattaa
     9241 cctgtctctg ggtttctctc atcctgttga ccatcagctg cctgcgggta tcactgctga
     9301 atgtccgact cagactgaaa tcgtgctgaa aggcgctgat aagcaggtga tcggccaggt
     9361 tgcagcggat ctgcgcgcct accgtcgtcc tgagccttac aaaggcaagg gtgttcgtta
     9421 cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag taaggtaaca ctatggataa
     9481 gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc aagctccagg agctgggcgc
     9541 aactcgcctg gtggtacatc gtaccccgcg tcacatttac gcacaggtaa ttgcaccgaa
     9601 cggttctgaa gttctggtag ctgcttctac tgtagaaaaa gctatcgctg aacaactgaa
     9661 gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa gctgtcgctg aacgcgctct
     9721 ggaaaaaggc atcaaagatg tatcctttga ccgttccggg ttccaatatc atggtcgtgt
     9781 ccaggcactg gcagatgctg cccgtgaagc tggccttcag ttctaaggta gaggtgtaag
     9841 atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa agctgatcgc ggtaaaccgc
     9901 gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca cagctctgac tgtagttggc
     9961 gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc gtgaagttcc agcagcgatc
    10021 cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg tcgcgctgaa taacggcact
    10081 ctacaacacc ctgttaaagg tgttcacacg ggttctcgcg tattcatgca gccggcttcc
    10141 gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg ttctggaagt cgctggggtt
    10201 cataacgttc tggctaaagc atatggttcc accaacccga tcaacgtggt tcgtgcaact
    10261 attgatggcc tggaaaatat gaattctcca gaaatggtcg ctgccaagcg tggtaaatcc
    10321 gttgaagaaa ttctggggaa ataaaccatg gcaaagacta ttaaaattac tcaaacccgc
    10381 agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc ttggcctggg tctgcgtcgt
    10441 attggtcaca ccgtagagcg cgaggatact cctgctattc gcggtatgat caacgcggtt
    10501 tccttcatgg ttaaagttga ggagtaagag atgcgtttaa atactctgtc tccggccgaa
    10561 ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg gttctggcct cggtaaaacc
    10621 ggtggtcgtg gtcacaaagg tcagaagtct cgttctggcg gtggcgtacg tcgcggtttc
    10681 gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat tcggcttcac ttctcgtaaa
    10741 gcagcgatta cagccgaagt tcgtctgtct gacctggcta aagtagaagg cggtgttgta
    10801 gacctgaaca cgctgaaagc ggctaacatt atcggtatcc agatcgagtt cgcgaaagtg
    10861 atcctggctg gcgaagtaac gactccggta actgttcgtg gcctgcgtgt tactaaaggc
    10921 gctcgtgctg ctatcgaagc tgctggcggt aaaatcgagg aataagtagc agatggctaa
    10981 acaaccggga ttagattttc aaagtgccaa aggtggctta ggcgagctga aacgcagact
    11041 gctgtttgtt atcggtgcgc tgattgtgtt ccgtattggc tcttttattc cgatccctgg
    11101 tattgatgcc gctgtacttg ccaaactgct tgagcaacag cgaggcacca tcattgagat
    11161 gtttaacatg ttctctggtg gtgctctcag ccgtgcttct atctttgctc tggggatcat
    11221 gccgtatatt tcggcgtcga tcattatcca gctgctgacg gtggttcacc caacgttggc
    11281 agaaattaag aaagaagggg agtctggtcg tcgtaagatc agccagtaca cccgttacgg
    11341 tactctggtg ctggcaatat tccagtcgat cggtattgct accggtctgc cgaatatgcc
    11401 tggtatgcaa ggcctggtga ttaacccggg ctttgcattc tacttcaccg ctgttgtaag
    11461 tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa cagatcactg aacgaggtat
    11521 cggcaacggt atttcaatca ttatcttcgc cggtattgtc gcgggactcc cgccagccat
    11581 tgcccatact atcgagcaag cgcgtcaagg cgacctgcac ttcctcgtgt tgctgttggt
    11641 tgcagtatta gtatttgcag tgacgttctt tgttgtgttt gttgagcgtg gtcaacgccg
    11701 cattgtggta aactacgcga aacgtcagca aggtcgtcgt gtctatgctg cacagagcac
    11761 acatttaccg ctgaaagtga atatggcggg ggtaatcccg gcaatcttcg cttccagtat
    11821 tattctgttc ccggcgacca tcgcgtcatg gttcgggggc ggtactggtt ggaactggct
    11881 gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt tatgtgttac tctatgcgtc
    11941 tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt ttcaacccgc gtgaaacagc
    12001 agataacctg aagaagtccg gtgcatttgt accaggaatt cgtccgggag agcaaacggc
    12061 gaagtatatc gataaagtaa tgacccgcct gaccctggtt ggtgcgctgt atattacctt
    12121 tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa gtaccgttct acttcggtgg
    12181 gacctcactg cttatcgttg ttgtcgtgat tatggacttt atggctcaag tgcaaactct
    12241 gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg aacctgaaag gctacggccg
    12301 ataattggtc gcccgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga
    12361 aattatgccg taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg
    12421 ccgagccgaa gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt
    12481 gggttgagct ggctagatta gccagccaat cttttgtatg tctgtgcgtt tccatttgag
    12541 tatcctgaaa acgggctttt cagcatggaa cgtacatatt aaatagtagg agtgcatagt
    12601 ggcccgtata gcaggcatta acattcctga tcataagcat gccgtaatcg cattaacttc
    12661 gatttatggc gtcggcaaga cccgttctaa agccatcctg gctgcagcgg gtatcgctga
    12721 agatgttaag atcagtgagc tgtctgaagg acaaatcgac acgctgcgtg acgaagttgc
    12781 caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc atgagcatca agcgcctgat
    12841 ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt ctcccggttc gcggtcagcg
    12901 taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa ccgatcaaga aataatcggg
    12961 gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa acgtgtaaga aaacaagtct
    13021 ctgacggcgt ggctcatatc catgcttctt tcaacaacac catcgtgact atcactgatc
    13081 gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc cggtttccgt ggttctcgca
    13141 aatccactcc gtttgcagct caggttgcag cagagcgttg cgctgacgcc gtgaaagaat
    13201 acggcatcaa gaatctggaa gttatggtta aaggtccggg tccaggccgc gaatctacta
    13261 ttcgtgctct gaacgccgca ggtttccgca tcactaacat tactgatgtg actccgatcc
    13321 ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata acgcctcgtt ttccaggttt
    13381 gttggagaaa gaaaatggca agatatttgg gtcctaagct caagctgagc cgtcgtgagg
    13441 gcaccgactt attccttaag tctggcgttc gcgcgatcga taccaagtgt aaaattgaac
    13501 aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc tgactatggt gtgcagttgc
    13561 gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga gcgtcagttc cgtaactact
    13621 acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa cctgttggct ctgctggaag
    13681 gtcgtctgga caacgttgta taccgtatgg gcttcggtgc cactcgtgca gaagcacgtc
    13741 agctggttag ccataaagca attatggtaa acggtcgtgt tgttaacatc gcttcttatc
    13801 aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc gaagaagcag tctcgcgtga
    13861 aagccgctct ggagctggct gagcagcgtg aaaagccaac ttggctggaa gttgatgctg
    13921 gcaagatgga aggtacgttt aagcgtaagc cggagcgttc tgatctgtct gcggacatta
    13981 acgaacacct gatcgtcgag ctttactcca agtaaagctt agtaccaaag agaggacaca
    14041 atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg ttgatatcga gcaagtgagt
    14101 tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg gctttggcca tactctgggt
    14161 aacgcactgc gccgtattct gctctcatcg atgccgggtt gcgcggtgac cgaggttgag
    14221 attgatggtg tactacatga gtacagcacc aaagaaggcg ttcaggaaga tatcctggaa
    14281 atcctgctca acctgaaagg gctggcggtg agagttcagg gcaaagatga agttattctt
    14341 accttgaata aatctggcat tggccctgtg actgcagccg atatcaccca cgacggtgat
    14401 gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga ccgatgagaa cgcgtctatt
    14461 agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc cggcttctac ccgaattcat
    14521 tcggaagaag atgagcgccc aatcggccgt ctgctggtcg acgcatgcta cagccctgtg
    14581 gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac agcgtaccga cctagacaag
    14641 ctggtcatcg aaatggaaac caacggcaca atcgatcctg aagaggcgat tcgtcgtgcg
    14701 gcaaccattc tggctgaaca actggaagct ttcgttgact tacgtgatgt acgtcagcct
    14761 gaagtgaaag aagagaaacc agagttcgat ccgatcctgc tgcgccctgt tgacgatctg
    14821 gaattgactg tccgctctgc taactgcctt aaagcagaag ctatccacta tatcggtgat
    14881 ctggtacagc gtaccgaggt tgagctcctt aaaacgccta accttggtaa aaaatctctt
    14941 actgagatta aagacgtgct ggcttcccgt ggactgtctc tgggcatgcg cctggaaaac
    15001 tggccaccgg caagcatcgc tgacgagtaa ccggatcaca ggttaaggtt ttactgagaa
    15061 ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg aaccgcaaca gcagccatcg
    15121 ccaggctatg ttccgcaata tggcaggttc actggttcgt catgaaatca tcaagacgac
    15181 tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg ctgattactc ttgccaagac
    15241 tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact cgtgataacg agatcgtggc
    15301 aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt gccggtggtt acactcgtat
    15361 tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg gcttacatcg agctggttga
    15421 tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa gcaacgtaaa aaaacccgcc
    15481 ccggcgggtt tttttatacc cgtagtatcc ccacttatct acaatagctg tactcttttt
    15541 gttcatcccc tggagtattt atgtggttac ttgaccagtg ggcagagcgc catatagcag
    15601 aagcgcaagc gaaaggtgag tttgataacc tggcaggtag cggcgaacca ttgatactgg
    15661 atgatgattc tcacgtgcca ccggaattac gtgcggggta tcgcttgctg aagaatgccg
    15721 gttgcttacc gccagaactt gagcaacgga gagaagcaat tcagcttctg gatattctca
    15781 aaggtatccg tcacgatgat ccgcaatatc aagaggttag ccgtcgattg tcattactgg
    15841 aattgaagct gcgacaagct ggattgagta ccgatttttt acgcggcgat tatgctgaca
    15901 agttgttgga caaaatcaac gataactagt ggagtatgta tgtatcgcat tggtgagctg
    15961 gcaaaaatgg cggaagtaac acctgacacg attcgttatt acgaaaaaca gcagatgatg
    16021 gagcatgaag tgcgtactga aggcgggttt cgcctgtata ccgaaagcga tctccagcga
    16081 ttgaaattta tccgccatgc cagacaacta ggtttcagtc tggagtcgat ccgcgagttg
    16141 ctgtcgatcc gcatcgatcc tgaacaccat acctgtcagg agtcaaaagg cattgtgcag
    16201 gaaagattgc aggaagtcga agcacggata gccgagttgc agagtatgca gcgttccttg
    16261 caacgcctta acgatgcctg ttgtgggacc gcccatagca gtgtttattg ctcgattctt
    16321 gaagctcttg aacaaggggc gagtggcgtt aagagtggct gttgattttt tgcactgaca
    16381 ggattacact cgcgccgtta aataaccaac tggagttttt atgagccgat atcagcatac
    16441 taaagggcag ataaaggata atgcgataga agcattacta catgatccct tattccgaca
    16501 gcgagtagag aaaaataaga aagggaaagg cagttacatg cgaaaaggaa aacatggcaa
    16561 tcgggggaac tgggaggcca gtggcaagaa agtaaatcac ttttttacca ctggtcttct
    16621 gctttcaggc gcttgttaag agcggttatt ctgctctttc agcaaatcac gaatttctgc
    16681 cagtaatact tcttctttag ttggtgcagg tgcggcagct ggttcttctt ttttccgatt
    16741 cagtttgttg attagcttaa tcgccataaa gatggcaaag gccacaatca gaaaatcaaa
    16801 gacgttttga atgaagacac cgtaatgcat cacaacagca gggatacccc cctgcgcatc
    16861 gcgtagcgtg acagcaaact gtttaaagtc gatcccgcca attaataagc ccagaggtgg
    16921 catgatgata tcggcaacca gtgaagatac aatcttcccg aatgccgcac cgataatgac
    16981 acccaccgcc aaatccacca cgttcccgcg cattgcaaat tcgcgaaatt ctttaataat
    17041 gctcatgtta ttctccctat gaagccgaca accataagtc taacaaatgt taagccattt
    17101 tcctgccagg aatgaattta attaatcctt gataatcaaa gggaaataat aaggcgtcat
    17161 tagacgcctt attaattaca agaagaaagg gcttggctgg aagagtcttt cgacgtcggt
    17221 aataaacttt ttatctgtga ggaacataat tacgtgatcg ccttgctcaa tgcgcagatt
    17281 gtcattggca atcatcacgt cgtttccacg taccaccgct ccaataatcg ttcctggcgg
    17341 tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag gtgctttcat caccgtgagc
    17401 aacggcttca atagcttctg ctacgccgcg gcgcaatgag gaaacaccaa caatatctgc
    17461 ttttcgcaca tggctaagca acgcagaaat agttgcttgt tgtggtgaaa ttgcaatatc
    17521 gatcacgctc ccctgaacca gatccacata agcgcgacgc tggatcaata ccatcacctt
    17581 tttcgcaccc atacgtttgg caagcatggc ggacatgata ttggcctcgt catcgttggt
    17641 gacggcaata aacagatcaa cttgatcgat atgttcttcg gccagcagtt cttgatccga
    17701 cgcatcacca aaaaagacga tcgtattctg caacttttcc gccagttcgg cagcgcgctg
    17761 ctgattacgt tcgatgagtt taacgctgta atctttttcc agacgacgcg ccagccctgc
    17821 accgatatta ccgccaccaa ccagcatgat ccgcttatac ggtttttcca gtcgctgtaa
    17881 ttcactcatc accgcgcgga tatgctgtga agcggcaata aagaacactt catcaccagc
    17941 ttcaacaatg gtcgaacctt gtggacgaat ggggcgatcg tggcggaaaa ttgctgccac
    18001 acgagtatcg atatgtggca tatgttcgcg catggtcgaa agtgcattac caatcagcgg
    18061 gccgccataa taggctttaa ctacagcgag gctgacttta ccctcagcga agttcaccac
    18121 ctgcaatgcg cctggatact caatcagtcg gtaaatatta tcgataacca actgctctgg
    18181 tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat agcttatcgg catcgcgtac
    18241 gtagtctggt gagcggatgc gagcgatgcg attaggggtg ttgaaaagtg agtaggctac
    18301 ctggcaggca accatattgg tttcatctga actggttaca gcaaccagca tatcggcgtc
    18361 gtcggcacct gcctcccgca atacgcgtgg atgagagcca tgcccctgca cgactcgcag
    18421 gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg gtatcgacaa cagtaatatc
    18481 gttgttctcg ccaaccaggt tttccgccag tgtgccgcca acctggccgg cacccagaat
    18541 gataattttc atcagtcgcg acccgttatc tcatcacttt ttgattagct tagcgtaaaa
    18601 gaagccgtcg ccctcttcgg caccaggctg attttgttta cccggttgct ctggtgttcc
    18661 tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag gctttaatct gcaggctatt
    18721 ctcttccggt aacaccgaac aggtggcata gaccagggtt ccaccggatt ttaaatgcgg
    18781 ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt tccgggatat cgcgatcgcg
    18841 acgtaaccat ttaatatctg gatggcgacg aatcaccccc gttgctgaac aaggcgcatc
    18901 taataaaatg cgatcaaact gttgctcgcc acaccattgg gaagggtaac ggccatcacc
    18961 ttgtttcacg gttgccttca taccaaggcg ttttaaattg tcgtaaacgc gagagaggcg
    19021 ctgttcgtca atatcaaccg caacaacctg cgcttctggt gccacctcaa ggatatgcgt
    19081 tgttttaccg ccgggggcgg cacaaagatc caagatgtgt tcaccgtttt gtggcgcaag
    19141 ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta acccatccgt cttcaaaacc
    19201 aggtagcgca tgaacaggtg caggtgtttc cagccgtaca gcatcagggt aatccgcatg
    19261 cgggaaacct ttcattcctg cttcatccag caatgcaagc cagctgtcgc gggaatgatg
    19321 cgtacgatta atacgcagcc acattggcgg acgctggtta ttggcttcga cgatggattg
    19381 ccactgctct ggatacgctt tttgcagacg cttcagcaac caggaaggat gcagataacg
    19441 tgcatcacgg gtattaaact cggctaataa ctcttcttgc cgacgctgga actggcgtaa
    19501 tacaccgtta atcaaccctt taagttgcgg acgcttaatt gcgatagcgc cttcaaccgt
    19561 ttcagccagc gcagcatgag gtggaatgcg ggtataaagc agttgataca aaccaaccat
    19621 aatcaggtaa tgcacagtgc gctgtttgcc ggtcatcgga cgggccatta acttattaat
    19681 cagccagtct aactgcgaaa gcgtacgcag tacgccaaag cacaactctt gaagaagtgc
    19741 tttgtcttta tcggaaactt tttgctggag cggtggcaga atgttgctta atgattgccc
    19801 ttgctcgacg acttgttcaa cggcctgggc cgccatgcta cgtaaattac gttgtttttt
    19861 cataagtata aaaatgctcg gcaagaccgg gcttagaaga gtggactatc agaccagacg
    19921 gttgcccgga acaaaccatt cccgacgaga gttcaggagg tcttgcgcgc tcatcgcttt
    19981 cttacccgca ggttgtaacg agagcaggtt caggatgcca tcaccagtcg caacctgaat
    20041 gccttgtttg ttggcttcaa ggatcgttcc tggtgcagcg ttggttgccg tatcaatgac
    20101 cgacgctttc cagactttaa ccggctgtcc ttcaatttcc agccagctca ttggccatgg
    20161 gttgaaagcg cgaatgcagc gttcaagctg tgctgccgaa agtgaccagt caatacgcgc
    20221 ttcttcttta ctcaacttct cggcgtaagt gacaagagtt tcgtcctgaa cttctggttt
    20281 cgccgtgccg tctgccaatt gtttcaacgt ggtgataagc ccttgtgggc caagctctgc
    20341 cagcttgtcg tacagcgtac cactggtatc ttctgcagta atcgggcagg agagcttata
    20401 gagcatgtca ccggtgtcta aaccaacatc catttgcata atggttacac cagtttctgc
    20461 atcacccgcc catagtgagc gttggattgg tgcagcaccg cgccagcgtg gcagcagtga
    20521 accatgaacg ttgatacagc caagacgcgg catctccagc acagctttag gcagaattaa
    20581 accataggcg acgacgacca taacatcagc ctgcaaatcg gcgaccagtt gctggttttc
    20641 ttgtggacgc agggaaacag gttgaaaaac gggcagacct tcttcctcag ccagaacttt
    20701 aatcgggctg ggcatcagtt ttttaccgcg tcctgccggt cggtctggct gggtgaacac
    20761 gccaacgacg ttatgaccag aagacaacag cgcgtcgaga tgacgcgctg caaagtcagg
    20821 tgtacccgca aaaataatac gtagtgattc tgacacgtta gttcttatcc ttaagcccgg
    20881 gctttcagac gatccagttt ttcaactttc tgacgaattc gttgttgttt cagcggtgac
    20941 agataatcca taaacagttt gccaaccagg tgatccatct catgctgaat acagatagct
    21001 aacagaccct ctgcttccag ttcaaatggt ttaccgtcgc ggtcaagggc gcgaatttta
    21061 actttctctg cgcgcggcac taaagcacgt tgttcaggga tcgacaggca accttcttca
    21121 atgcctgttt cgccgctttt ttctaaaagc tccggattga ttaacaccag ccgttcgtca
    21181 cggttttccg aaacatcaat aacaatgata cgttgatgga tatcaacctg ggttgccgcc
    21241 aggccaatac cttcttctgc gtacatcgtc tcgaacatat catcgacgat acgctgaatt
    21301 tctgcattca cttcttctac cggtttagca actttgcgaa gccgctcgtc cggaatatgt
    21361 aacacttgca aaactgacat aaatctccag agatgtgttc aggagttaga aagattattt
    21421 cttctattct agacaaatcc ccctctgatt gacagcatca ctgaccaatc gcaaagattg
    21481 ctaaggcttg cttatggcag ggagataagg atggtcgata cagaaatttg gctgcgttta
    21541 atgagcatca gcagcttgta cggcgatgat atggtccgta tcgctcactg gctggcaaaa
    21601 cagtcgcata ttgatgcggt tgtattgcag caaacagggc ttacattgcg gcaggcacaa
    21661 cgctttcttt catttccacg aaagagtatc gaaagctcac tttgttggtt ggagcaaccc
    21721 aaccatcatt taatccctgc ggacagcgaa ttttatcctc ctcaacttca ggcgacgaca
    21781 gattaccccg gcgcactgtt tgttgaagga gaactgcacg cgctgcattc atttcagctt
    21841 gccgtagtgg ggagtcgggc gcattcatgg tatggcgagc gatgggggcg attattttgc
    21901 gaaactctgg cgacgtgtgg agtgacaatt acgagtggac tggcgcgtgg aatcgatggt
    21961 gtggtgcata aagcggcctt acaggtaaat ggcgtcagca ttgctgtatt ggggaatgga
    22021 cttaatacca ttcatccgcg tcgccatgcc cgactggctg ccagtctgtt tgaacagggt
    22081 ggcgctctcg tctcggaatt tcccctcgat gttccacccc ttgcttacaa tttcccacga
    22141 agaaatcgca ttatcagtgg tctaagtaaa ggtgtactgg tggtggaagc ggctttgcgc
    22201 agtggtttgc tggtgacagc acgttgtgcg cttgagcagg ggcgtgaagt ttttgccttg
    22261 ccaggtccaa tagggaatcc gggaagcgaa gggcctcact ggttaataaa acaaggtgcg
    22321 attcttgtga cggaaccgga agaaattctg gaaaacttgc aatttggatt gcactggttg
    22381 ccagacgccc ctgaaaattc attttattca ccagatcagc aagacgtggc attgccattt
    22441 cctgagctcc tggctaacgt aggagatgag gtaacacctg ttgacgtcgt cgctgaacgt
    22501 gccggccaac ctgtgccaga ggtagttact caactactcg aactggagtt agcaggatgg
    22561 atcgcagctg tacccggcgg ctatgtccga ttgaggaggg catgccatgt tcgacgtact
    22621 aatgtatttg tttgaaacct atattcacac tgaagctgag ttgcgtgtgg atcaagacaa
    22681 acttgaacag gatcttaccg acgcaggttt tgagcgagaa gatatctaca atgccctgct
    22741 atggctggaa aaacttgctg attatcagga agggttggca gaaccgatgc aactggcctc
    22801 ggatcctctc tccatgcgta tttatacacc ggaagagtgt gaacgactgg atgccagctg
    22861 ccgtgggttt ctgcttttcc ttgagcagat tcaggtgctc aaccttgaaa ctcgtgaaat
    22921 ggtgatagag cgagtgctcg cgctggataa cgcagagttc gaactggatg atctgaaatg
    22981 ggtgatcctg atggtgttgt tcaacatccc gggctgcgaa aatgcgtacc agcaaatgga
    23041 agaattactc tttgaagtga atgaaggtat gctgcattaa tcatttcttt aattcagcat
    23101 aagttgttat ggcaaaatca gcactgttca cggtgcgtaa taatgagtcc tgcccaaagt
    23161 gcggggctga actggttatt cgatccggaa aacacggtcc gtttcttgga tgctcacagt
    23221 atccggcgtg tgactacgtc cgtcctctga aatcttcagc ggatggacat attgtcaaag
    23281 ttctggaggg gcaggtttgc cccgtatgtg gcgcaaatct ggtattacgc cagggacgct
    23341 ttggtatgtt tattggttgc agtaactacc ctgaatgcga acataccgaa cttatcgata
    23401 aaccggacga aacagcaatt acatgccccc aatgtcggac gggccatctg gtccagcgcc
    23461 gctcccgtta tggcaaaaca tttcactcct gtgatcgcta cccggagtgt caatttgcca
    23521 ttaacttcaa accaatagct ggagaatgcc ctgagtgtca ttatccgcta ctcatcgaaa
    23581 agaaaaccgc gcagggtgta aaacactttt gtgccagtaa acaatgtgga aagccggttt
    23641 cggcggaata ataacgtgaa taataacctg caaggagacg ctatcgcagc tgcgatagat
    23701 gtcctcaatg aaaaacgtgt catcgcctat ccaacggaag ccgttttcgg tgtcgggtgc
    23761 gatcctgata gcgaaacagc agtgatgaga ctgttggagt taaaacagcg tccggttgat
    23821 aaggggctga ttttaatcgc ggcaaattac gagcagctta aaccctatat tgatgacacc
    23881 atgttgactg acgtgcagcg tgaaaccatt ttttcctgct ggccaggtcc tgtcaccttt
    23941 gtctttcccg cgcctgcgac aacaccgcgc tggttgacgg gccgctttga ttcgcttgct
    24001 gtacgagtca ccgaccatcc gttggtggtt gctttgtgcc aggcttatgg taaaccgctg
    24061 gtttctacca gtgccaactt gagtggattg ccaccttgtc gaacagtaga cgaagttcgc
    24121 gcacaatttg gcgcggcgtt cccggttgtg cctggtgaaa cgggggggcg tttaaatcct
    24181 tcagaaatcc gcgatgccct gacgggtgaa ctgtttcgac aggggtaaca taatggaaac
    24241 ctatgctgtt tttggtaatc cgatagccca cagcaaatcg ccattcattc atcagcaatt
    24301 tgctcagcaa ctgaatattg aacatcccta tgggcgcgtg ttggcaccca tcaatgattt
    24361 catcaacaca ctgaacgctt tctttcgtgc tggtggtaaa ggtgcgaatg tgacggtgcc
    24421 ttttaaagaa gaggcttttg ccagagcgga tgagcttact gaacgggcag cgttggctgg
    24481 tgcagttaat accctcaagc ggttagaaga tggacgcctg ctgggtgaca ataccgatgg
    24541 tgtaggcttg ttaagcgatc tggaacgtct gtcttttatc cgccctggtt tacgtattct
    24601 gcttatcggc gctggtggag catctcgcgg cgtactactg ccacttcttt ccctggactg
    24661 tgcggtgaca atcactaatc ggacggtatc ccgcgcggaa gaggtggcta aattgtttgc
    24721 gcacactggc agtattcagg cgttgggtat ggacgaactg gaaggtcatg agtttgatct
    24781 cattattaat gcaacatcca gtggcatcag tggtgatatt ccggcgatcc cgtcatcgct
    24841 cattcatcca ggcatttatt gctatgacat gttctatcag aaaggaaaaa ctccttttct
    24901 ggcatggtgt gagcagcgag gctcaaagcg taatgctgat ggtttaggaa tgctggtggc
    24961 acaggcggct catgcctttc ttctctggca cggtgttctg cctgacgtag aaccagttat
    25021 aaagcaattg caggaggaat tgtccgcgtg aatcaggcca tccagtttcc ggacagggaa
    25081 gagtgggacg agaataaaaa atgtgtatgt tttcccgctc tcgtgaatgg tatgcaactg
    25141 acatgcgcga tctctggcga gagtctggcg tatcgcttta ctggagatac gccagaacag
    25201 tggttagcga gttttcgtca gcatcgctgg gacctggaag aagaagcgga aaacttaatt
    25261 caggaacaaa gtgaagatga tcaaggctgg gtctggttac cctgatccag atattcgtcc
    25321 ttccatttca cgtaattatt cgcggaatag cgtaacccag ccttctcttc atcacttaac
    25381 gggcggatct gtttgacggg gctaccgaga tacagatatc cgctctccag ccgtttattt
    25441 tgtgggacca gactacccgc accaatcatc acatcatctt ctactattgc gccatcaagt
    25501 aaaattgagc ccatcccaac caaaactcga ttgccaatgg tgcagccgtg gagcatcacc
    25561 ttgtgaccaa cagtgacatc ttcgccaatg gttaatgggt tgccatctgg gttgtacgag
    25621 gatttatgag tgacatgcaa catactgcca tcctggatat tggtgcgtgc tccgatctgt
    25681 acataatgta catctccacg aatcacaacg agcggccaga tccccacatc atcagccaga
    25741 cgaacgtcac caatcacgac actgctatcg tcgatcatta cgcgctgacc gatttgtgga
    25801 aaaagatcgc ggtatgggcg taaaacatca gacatactta cctcagcaat aaatgattta
    25861 ctaatgactt tgggggcatt attggccttg tgcaagtctt ttagtatgca aaaaagcacc
    25921 gttttgtgtg cgattgcagc aaaaagggtg aaaaaacaac aaacagaaaa aaagatcaaa
    25981 aaaatacttg tgcaaaatat tgggatccct ataatgcgcc tccgttgaga cgacaacgtg
    26041 aaacacttca caggatggtc ggaacaacga agagaaaaaa tcctgaaatt cagggttgac
    26101 tctgaaagag gaaagcgtaa tatacgccac ctcgcgacag tgagctgaaa g
//
LOCUS       DADLSB010000043        25652 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.043, whole genome shotgun sequence.
ACCESSION   DADLSB010000043 DADLSB010000000
VERSION     DADLSB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.043"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..602)
                     /locus_tag="JN331_001766"
     CDS             complement(<1..602)
                     /locus_tag="JN331_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7259932.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALVVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1050..2021
                     /locus_tag="JN331_001767"
     CDS             1050..2021
                     /locus_tag="JN331_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7259933.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            2070..3083
                     /gene="yhjD"
                     /locus_tag="JN331_001768"
     CDS             2070..3083
                     /gene="yhjD"
                     /locus_tag="JN331_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY7259934.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3494..4816
                     /locus_tag="JN331_001769"
     CDS             3494..4816
                     /locus_tag="JN331_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY7259935.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4998..7058)
                     /locus_tag="JN331_001770"
     CDS             complement(4998..7058)
                     /locus_tag="JN331_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7259936.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLTPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="JN331_001771"
     CDS             complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="JN331_001771"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY7259937.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQLLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            7908..9056
                     /locus_tag="JN331_001772"
     CDS             7908..9056
                     /locus_tag="JN331_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7259938.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKGADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9152..10648)
                     /locus_tag="JN331_001773"
     CDS             complement(9152..10648)
                     /locus_tag="JN331_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7259939.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADSLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10869..12155)
                     /locus_tag="JN331_001774"
     CDS             complement(10869..12155)
                     /locus_tag="JN331_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY7259940.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVEVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(12339..14327)
                     /gene="hmsP"
                     /locus_tag="JN331_001775"
     CDS             complement(12339..14327)
                     /gene="hmsP"
                     /locus_tag="JN331_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY7259941.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVNAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(14409..17882)
                     /gene="bcsC"
                     /locus_tag="JN331_001776"
                     /pseudo
     CDS             complement(14409..17882)
                     /gene="bcsC"
                     /locus_tag="JN331_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            complement(17864..18970)
                     /gene="bcsZ"
                     /locus_tag="JN331_001777"
     CDS             complement(17864..18970)
                     /gene="bcsZ"
                     /locus_tag="JN331_001777"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY7259942.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTGPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQDWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(18977..21316)
                     /gene="bcsB"
                     /locus_tag="JN331_001778"
     CDS             complement(18977..21316)
                     /gene="bcsB"
                     /locus_tag="JN331_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY7259943.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNKFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLSTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(21327..23929)
                     /gene="bcsA"
                     /locus_tag="JN331_001779"
                     /pseudo
     CDS             complement(21327..23929)
                     /gene="bcsA"
                     /locus_tag="JN331_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            complement(23926..24678)
                     /gene="bcsQ"
                     /locus_tag="JN331_001780"
     CDS             complement(23926..24678)
                     /gene="bcsQ"
                     /locus_tag="JN331_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY7259944.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKANGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(24690..24878)
                     /locus_tag="JN331_001781"
     CDS             complement(24690..24878)
                     /locus_tag="JN331_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259945.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            25151..>25652
                     /locus_tag="JN331_001782"
     CDS             25151..>25652
                     /locus_tag="JN331_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7259946.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRS"
BASE COUNT         6015 a         7101 c         6460 g         6076 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccaccaac gcaaatgtct cgacggttga atcgcgaggc
      301 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga ttttcattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
      781 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
      841 aaattccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
      901 gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg
      961 gattcacctt tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     1021 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat
     1081 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     1141 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg
     1201 ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     1261 agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     1321 aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg
     1381 ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt
     1441 tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     1501 gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     1561 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg
     1621 ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac
     1681 acgcaatagt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     1741 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     1801 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     1861 gcgaagccct gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc
     1921 tctccctttc gctggtttac ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     1981 tgcagtggct ggctggcgta atgaaagagc acctggactg accgactata ctttttaaga
     2041 atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     2101 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     2161 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     2221 ttgcgcacct tattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     2281 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     2341 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     2401 tgcaaaacat cagcgatccg acactagccg ccacgttgaa aaacaccatc aacaccgccg
     2461 ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcactttat tccggcatca
     2521 actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct
     2581 cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcactgattg
     2641 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     2701 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcgat
     2761 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     2821 ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     2881 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc
     2941 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     3001 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     3061 tgccagggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat
     3121 acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg
     3181 ctataatgcg cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg
     3241 ggagcaaaca atttcactcc aactcataac cccagcatat aaatccagtt ggtaactttt
     3301 atttaacctg aaaccagttt tatccactat ttataaaatt atgtgaagca tttcatagaa
     3361 gaaaaatcac tggcctaaac attatcccct ttttgcctgg tttttgacca tttccgcgat
     3421 ttgttacaca ttgaaatatc acttttgctg tgcgtaatat ggctattcgt tagccaaaaa
     3481 ataagaaaag attatgcaag caacagccac aacactcgac cacgaacaag aatacacgcc
     3541 gatcaactcg cgtaataaag tccttgtcgc ctctctcatt ggcacggcca ttgagttctt
     3601 cgacttttac atttacgcca ctgcggccgt tattgtgttt ccgcatatct tcttcccgca
     3661 gggcgatcca acggcagcaa cgctacagtc gctcgccacc ttcgccatcg ccttcgtcgc
     3721 gcgccccatt ggctctgccg tttttggtca ttttggcgat cgcgttgggc gtaaagcgac
     3781 gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc tgctgccggg
     3841 ctatgccacg attggtattt tcgccccact gctgctggcg ctggctcgat ttggtcaggg
     3901 tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc
     3961 gcgcaaacgt gcactgtatg gctccttccc acagctaggc gcaccgattg gcttcttctt
     4021 tgccaacggc actttcttgc tgctttcctg gctactgacc gatgagcagt ttatgagctg
     4081 gggctggcgt gtgccattta tcttctcggc ggtgctggtc attatcggcc tgtatgttcg
     4141 cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa
     4201 gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg gtacgttcat
     4261 tatgctggca acctatacgc tgttttacat catgacggtc tactctatga ccttcagtac
     4321 cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat
     4381 ggcagttatt ggttttggcg tgatggtgcc agtcgccgga ttactggctg atgcctttgg
     4441 tcgccgtaaa agcatggtga tcatcaccac gctgatcatc ctgttcgcgc tgttcgcctt
     4501 taacccgctg ctcggttctg gcaacccgat tctggttttt gccttcctgc tgctggggtt
     4561 aagtctgatg gggctgacct tcgggccgat gggtgcgctg ttaccagagc tgtttccgac
     4621 agaagtgcgt tacaccggag catcgttctc ttacaacgta tcgtcgattc tcggggcttc
     4681 cgttgcgccg tatatcgcag cctggttgca ggctaactac gggttaggtg cggtggggtt
     4741 atatctggcg gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc atgagacgcg
     4801 acatcagtcg ttgtaataag tgtgaaaagc ctgatgcgct acgcttatca ggactgaatt
     4861 tccatacaat atattgaatt cacaaggatt tgtaggccgg ataaggcgtt cacgccgcat
     4921 ccggcatgaa caaagcacac tttgccaaaa atctaaatct gtttaattgc cggatgtcag
     4981 acatccggca attatcatca cttcttcatc tgcgacaata tcgtccgaca ctgattagcc
     5041 tccccttcgg aaggtgagat cagtgccagt aacgccgccg ccggtgttac cagcgttgcc
     5101 agtgccgcag caacagcacc acgggcaatc agcggtccgg ctttcacccc agcctgcgga
     5161 tttttaaacg tcccacgcac atacagcggc gaacgcagtg tgataatccg aatcccttta
     5221 ctctccggat caatagtcaa atccagctgt tccgaagcaa aacttgccgt gccggtaaca
     5281 ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg ggcgcgccac gccgttggca
     5341 atattcagat tcgccgccgc gcagttcacc cgcacctcat cgtcaccaaa tatcgcaccg
     5401 acaatgtagt tgccgacatt cagcccaaca atctccatca ggttgcggct caccagcccg
     5461 tcattcatca acagtttcag gttgccgtta ctgttgccta aaagcgccgc caccgagtta
     5521 ccgctaccgc gtagttccgc gtcaccgttc atttccccca gcgtcttctg catcagttcc
     5581 acatcgggca tcagttcttt cagtttcagt cgacgagcct gaatatctgc ccgcccctgc
     5641 atcggctttt tatcgccttc cagatgaata ttcgccgcaa tgctgccgcc cgccatgcca
     5701 aatttcagcg gttgcaggcg caggtcagca tttttgagga tgatatgagt agaaagatcg
     5761 ctaatcggca ggctactgcc atgctcaatg cgccgccctt tgaagcgaac atcggcatcc
     5821 ataacgtccc atttatcggt ttcgaagcgg tcataaggca gcactttgcc cgcaggctga
     5881 acgctttttt cgcccttctt ctgttcagac cgtttcgact tttctgcccc tttcccggaa
     5941 tcaacgccaa tcaacggtcc caggtccgcc agccgcaatt gccgcgactc gacatcacct
     6001 tccagttttg gtcgtggctt tccggtggtg tagaccagag aaccgtggat atcgctatcg
     6061 ccaattcgcc cattaaaacc gcgataatca aagaccgacg atttttcagt gtcgattttc
     6121 gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt
     6181 tcatagagat cacccagtga atcgccagaa aatttaagcc gtaaatcgac accgcccatc
     6241 ttcattgggt cattcacgac gccatcaaaa gcaacacggg tattacctga acggaaatca
     6301 gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc tatttttccc
     6361 gtacccgtga gcggttcacc gttataacgt ccctgcgcct tcaggccaaa aatgtaatcg
     6421 cccacctttt ctttatccgc tttacctttc gatccagtaa cttcgctgaa tggcagcggc
     6481 ttgcctaacg gatcaacaaa aatttccaga tccgctttgc ttactttgtc atcaatggcg
     6541 atccgccctt gatcgaaaag aatattatcc agccgaaacg accatgccga cggctttgca
     6601 ttcgcgtctt tgttgtcatc gttggcaaga ttaaacgtcc agttattgtt cttttcagag
     6661 aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca gaccgttttg
     6721 gtcagcagcg ccagcggtgt cagcgttgcc tctactcgcg gcaaatgcac catcgtgact
     6781 tcgggaatat ccggtggatt accaagaatg atgtcttctg catgtacatg aggccacggc
     6841 acccagctgc gccagccagt ttcttgtttt tgccgctccc acaccacgcc cagatcgcca
     6901 cggatagcga acggacgatt caactccgca gagactttct ggttgatggt cggtttgagt
     6961 cgattccagt caaaagttgc aatcaaaatg atcgccacga caatcaacaa caagaaagcc
     7021 cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct catattcttc
     7081 ctgtgccagt cctaaagata gtccagcccg gcggaaaatg aggcagctta tagcgccaga
     7141 accgccgtat tcagcgtttc catcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg
     7201 aatgcgggcg agttctgaac atcacgccac tcttccggtg tttctacgcc ctcgacaatc
     7261 accccgcgac aatagcgatt cattagatgt aaaagctgag aaaagagtgt gcggccttcc
     7321 ggcgactgac gcagcatcac aaacagttct cgcgcgattt tgatgtagtc ataacgcact
     7381 tcacttagtg cagagaaatt tgccatcccg gtaccaaaat catccagcca cagcgggcca
     7441 aattcacaca tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc caccagttcg
     7501 aaacgcagcc agggaagacg ctcaatctgg cgcaggattt ttggttgctg acgcagggcg
     7561 atgagcgtag ggccatcaat attgaccgat gccagcaggc cgtgctctac aaagaagtcg
     7621 gctttttgcg ccagcaaatc aatctgctct ttcacaacct ccatccgatg gctgacggtg
     7681 atttcagtaa aatagcgatc cggcggcagg agttgcgaag ggttcaaggg atgcgtgacc
     7741 accgttaata gctccacggc cattaaccgc ccgcatgttt gatagatcgg ctgccaggtg
     7801 taagcacgct cacactgcaa ccaaaaacgc cgttcctgca agctctcgat gcttgcttca
     7861 gggttgctta ttcgctggat aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg
     7921 atgctgcccg gactcgacaa agattatcgg cgcgtaaggg cagaacttta ccgcctggcc
     7981 ggggtaaatt tcctcactcc acaacggaat ctttcaggaa tacgttctgg ctcaaaaaaa
     8041 taatggaaca ctgttttaat atggttgacc agcaaaccac cacagcgcaa actaacgcta
     8101 attttttaca gatcaggttc acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca
     8161 tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc gataccctga
     8221 acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa
     8281 cggcgctggg aacggacagt tttagccagc agatgctgga cgcctggcac ggcgagaacg
     8341 ttgatacttc cctgacccaa cggatggaaa accgtcttcc gggcctttac tacattgaaa
     8401 ccgacagcac cggcgaacgt acgttctact actggcgtaa cgaagccgcc gccaaattct
     8461 ggctggcgag cgagcagtct gcggcgattt gcgaagagct ggcgaatttc gattatctct
     8521 acctgagcgg gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag ctgctttccc
     8581 tgctgcgcga atgccgcgcc aaaggcggaa aagtgatttt cgacaataac tatcgtccgc
     8641 gcctgtgggc cagcaaagaa gagacacagc aggtgtacca gcaaatgctg gaatgcacgg
     8701 atatcgcctt cctgacgctg gacgacgaag acgcgctgtg gggtcaacag ccggtggaag
     8761 acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg
     8821 attcttgcct ggtgtccatt gctggtgaag cgttagttga tgttccggcg gtgaaactgc
     8881 cgaaagaaaa agtgatcgat accaccgcag ctggcgactc tttcagcgcc ggttatctgg
     8941 cggtacgttt gacgggcggc agtgcggaaa acgcggcgaa acgcgggcac ctgaccgcaa
     9001 gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca gcgtaaacgc
     9061 agcatatgca aaaggctgga taaagtgtta cccctctatc cagctcatcc ataaatacct
     9121 gatggttgtg ctaactcatc accatcaggc attactgtgc aggtggaata tccgtcactt
     9181 caggatgtac gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat ggggccatga
     9241 tttgatccca ggccgcctgc aacgctttca tgttaaattc cggctcgcct ttcggctgca
     9301 gcagtattaa cgccatatca ttcgacaact gctgacgcag atcctgattt aacatctcca
     9361 cagtcaggct gttaaggaaa tcctgccgca gtttctgata ctgctccggc gcgatatcga
     9421 cgacctgatt ttgcaacgaa cgcatccgct ggcccatcag aatatcggta tcagcccgtg
     9481 cataggcggc aaacagtttc tgcagctcca gtttcttttg cgccactaac gcattgaact
     9541 cttcttccgg cagaccttta tcgcgaacct tcgccagttc acgcgccacc agattaaggt
     9601 tgctgttcag cttgtcgttt ggtgattcga tgttgatggc acactgcgca cgcagataca
     9661 gcacacggca gtcaaatcca agaccgatgt ctttgctgtc actggcgctt aacgcttgct
     9721 gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg ccagtagcgc agcagtgcgg
     9781 ctgattcacg aatcggctgc cacggcgtat cccacatgat agataaccgg tcctgacgca
     9841 ccgcgtcagt cataatactc accgcttccg cacgcagcgg agaaagcgtc ggcaccggag
     9901 ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt gttgatttgg tcgacaaccg
     9961 agcgcgcatc cacgtttccc accaccagta gcgtcattgc atccggggtg taccatttct
    10021 gatagaaatc tttaattttt tccgcttcaa cgggttgttt cagcgaatcg gcaggatcgt
    10081 gacctaacaa ggttgaccct ttcagacgat agcgccacca gccctcttta gtatcggcag
    10141 gccaggttgc caccatgtcc tgactttgca gcgcgtggtt gatggtttct ggtgtgatgg
    10201 tcaatttgcc tgtggcattt gccagataag agagcgcttc tttcagcaaa tcgttacggt
    10261 tattgggcaa actcagatta aacagcgtgg tgtcataaga gacaattacc ggcggcatcg
    10321 ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg tcaaggccac
    10381 cgctttgcgt tagcgcaata cgagggatgg cgtgactgta accgctctgt tgtgtacttt
    10441 cggcgagcga accggtatta accagcaggc gaatttcaac acgatcgctg ggacgctggg
    10501 gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc catgctggat
    10561 caggctggag cgcatctgcc tgcacatagc cagcagtggc catcatcagc aaaccgcccg
    10621 ctaaaagtcg aatttttatg ccctgcatgt gaacccctga tcaactatcc tggtaataaa
    10681 aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa
    10741 gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc
    10801 aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag
    10861 tggtgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca
    10921 tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg
    10981 ccgttaccga ccaggttagt tagcgcacga gcttctgaca taaagcggtc gataccgagg
    11041 atcagcgcca gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg
    11101 ataaagccac tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac
    11161 agcgtgattt ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc
    11221 gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc
    11281 agccccacca ccgatttacg gcagccgagt ttctccatct tgtcgagcat acgcggcagc
    11341 gccgactcgg aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata
    11401 aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac
    11461 aggatacagg taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg
    11521 ccgtatttac cgatggtaaa cgccattgcc ccgaacgcac caataggtgc cagacgcatg
    11581 atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt
    11641 tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc
    11701 tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg
    11761 aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa cctctaccgc tttcgcatca
    11821 agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga
    11881 ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta
    11941 cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg
    12001 acaggagcga tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc
    12061 tcgcctattt caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc
    12121 tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag
    12181 gtcttgtaag gttatggggg tacctgcggc cttaaaataa cacccagaca acatcacaga
    12241 aatgtacctg gatcataatt gaaacaaaaa ggttaaaaag tttgagctgg ctcgcacaaa
    12301 ccagcacttt ttaaagtttt gtaatcagtt tggggtagct acttttcttc caggtaactc
    12361 tcttcgaaga tttcaatagg gagtgggcga gcaaaaagga agccctgggc aataccaacg
    12421 cccgcttttg ccagccagtc tcgttgtgct tcagtctcca cgccttcggc aatcatttgt
    12481 aagttcaggc tctgcgccag catgataatt gcagcaatca tgctgctatc ttccggcaag
    12541 ccttcaacaa acattttgtc gattttcagt acgtcgattg gcaacgattt catatgctgc
    12601 agctgacgca gccctgcgta gcccatgccg aaatcatcca gcgccacccg aactccggca
    12661 ttgcgcagcg gacggaggat tgccaccgca gcatgagggt cgtcaatacg tcggctttct
    12721 gtcacttcca gaatcagtgt tcccggctga atgcgatagc gggttaacag ttccagcata
    12781 tccgccacca tattcgggtg catcagttgc agcgcagaga ggtttaccga caagggcagc
    12841 ataatgccgc gctcttgcca ggctgcaagc aatcgacagg actcttccag cacccagtga
    12901 ccgacggtaa ccatcagccc acagcactca atgcgatcga ttaagccatc cggcaggtcc
    12961 caactgccat ccggttgctg gatacgcagt aacacttccg cattgaccag tttaccgctg
    13021 gtcatctcga cctgtggctg taaccaaatt gcaaactgat gattttccag tgcattaagg
    13081 atatcgctct cttccgtcaa ccgcttctgg gctgcttcca tctgctgcgg atcaaagaac
    13141 tgaatctgat tcttgccttt atggcgagcg gtaaatgccg cagaaatagc gcgactgtaa
    13201 agctgttcgg cggtgagatc gccgtagaac atcgccacgc caatgctaca gtgcggacgg
    13261 agttgaatac gttcaatcgg caggcgctcg ctcatgatag tgagtacttg ctgacccaaa
    13321 gtgattgcgt gccacggttc ctgtacaccg ttggcaatga cagcaaagtc ataaccgcta
    13381 atctgcgcga ggatcatacg tggcgacagt accgatttga gtttttccac cagcgtcagc
    13441 agcagaattt ctcgttgcgc ctctttcagc acgcccgcag tatcacgcag ggtttcacag
    13501 gtgatgatca tcagcgcggt ggtttgttta cgcgcaacaa cctgctccag catctccatc
    13561 agcaaggctt tgttcggcaa atccgacacc gggaagcgca tcgcattttc gttctgttct
    13621 tcataatggc gctgcagcaa ttgctggttg aggttgtaac tgcgcaccaa cataccgatt
    13681 tcatcgtcct gatgcagacg cggtaatgcc agttggtgac caacaagctc ctgggctggg
    13741 atggcgttaa gttcgcgagc aatattgcgt aacggatgca aaatcaggcg gttaatgcac
    13801 cagctgatgg cgacggtcag gataagcgac aaaagtaagt aaatggtcac taacgttgag
    13861 agagtgctca tcacgaactt atacatacgg aaggaatccg cctgtagcac cagataggct
    13921 attggctgcg gatttgccgg acgttcgagc gagtagacgc ccagcgagat ttgaaccggt
    13981 agctcaaaca ggcgagtaac cattaccggc accggacgct ctggaataaa acttttacgc
    14041 agcgcctgga actggttagg cagcactaca tcggcacggc tgaccacgcc tgccggttta
    14101 atgctggcaa gaatagcttc cgcttcggga atatcgcctt tcaaaatagc tgaagataag
    14161 ggttcgcgga cagagcgagc aatgctttcc agttgcgtag ccgtgttata gcgattctgc
    14221 tggaccagat ggaacagcaa aacggtgcaa aaaataaaaa cgaacaccag gacaacggct
    14281 gccaccattg ccatctgctt gattgttaac gagcgactta cgcgcaaatt gtctcccccc
    14341 ggaaatccca gattgccgcc cgagtatacc cgatcgcagc ggcattaaga gaggcgctat
    14401 ctgaaaactt accagtcggc gtaaggtatc agcggctgcg gcggtaaatc catgtcaccc
    14461 tgccatccgg cggcggaata acgtacgtag agcagggaat ggctgggtgc gtaatctttc
    14521 gcctgctgga tatcaattgc cgtgccaaca aaccagttgg aagtaacacg tcgttcaagt
    14581 aatgcccgcg ccgtgtagcc gaagccctga ctgctgccgc catcgttgga ttgccgcgca
    14641 gcttcttcct gccagtcggt cgggatcaga ttcatcagcg gataacgcgg catggttttg
    14701 gtgcgtgaat gcgaccacga gccagacgcc cccagctccc acgaccagtt ttccgtgcgc
    14761 tcccgccaca tcaccggtat ggcaaacgac aggtattcct gcggactgta gtaaccgccc
    14821 tgaccgagtg agtagccact cagatctttg tcgtaatgcc agatcatgtt gttcaggccg
    14881 attgtgacgc ggcgattgtt ctggttaatg accttatagt aatagcccgt catccagcgc
    14941 acgcgccagt tatcttcgac atttttaccg gttaactggt cgccactaag cgatgcccag
    15001 acgccgtttg cttcaccttt atcgtagctc agacttagcc ccacaccgtc ggcacgtacg
    15061 ccaccccatt ttttcccggt attgctcggg gagtcttttt gcccaccaaa ggccagcaaa
    15121 gaactggaga tgggccgacg gtgggcgtta acggtgtaac ccagcggccc gatatcatcg
    15181 ctgtaactga tgccgccgac cacatccacc acgttgaagc ccatcggcgt ggtaccgata
    15241 tcccagctcc agacgtcatt tcgccagccg accgccacgc tggcaccgga atccgactgg
    15301 ctgcggttgc cgctacagtc ctgtaatgta caggtgcccc agttgtcatc ccatttgcca
    15361 tcggcattag tggagaaact gccgacgttc atattgacga aatcactgcg aaagaacatc
    15421 cgcccgtcag aatacggcgc atccacctgc aacatggtag tgtgcgcttt cagatcggag
    15481 taaccaccgg tgccgctcga accccagtaa tcgtgctcaa gggtgacgtt aagatcctgc
    15541 tggcgataga ggtccgccgc atcgctgcgc acgccgcgtt tcagccagtc atctttctcg
    15601 tcgttacggg tcagtcgggt aaaggtgtcg ttatcctgcg gacgcgtcgt agtcacaccg
    15661 gatgcgacca tggcgtcttt gtaggtttcc agcgcctgct ttggatcgcc cgcctgcgct
    15721 tcaaacttcg caccgtcgcg cagcaccatc gcgctttcca tcgacggtgg ctgagatttt
    15781 gcctgcggga tcaacttatt aaacgtctgc tgcgccgctg cggtatctcc aagctgcgcc
    15841 tgtgccagcg ccacgcgccg ctgagtgttc agcgaggtgt tatcggtggc aggcagtttc
    15901 gccagctggc tacgtgccgc cgctttgtca ccggcagcaa tatccacttc cgtcagacca
    15961 agaatggcat cggcgttagt tggctcccgc gtcaggacat tctgatatgc agtgcgggcg
    16021 gcggtgtaat cacgtcgttg ttacgcccag tcagccagcg tgaggtcaat acgcgtggaa
    16081 ggtggttgct gacgcagcat cgcttccgct tcagcctctt tgccgctttc tcgcaggcga
    16141 ttagcggttt ccagcacctg atcgctttgc agtcgattaa ccagctcctg aatattgctg
    16201 ttccactgcg cgcgcggcag gctgttgata tgcgccagcg ccgctctgtc ctggttgtga
    16261 cctgagaggt acagcccgta agcgtaaacc tgctccgggt cgttcggctt ctgctgcgcc
    16321 agattgcgca ttaacgtatc ggcctggctg cgttgtccgg cctgccagag atcctgcgaa
    16381 agtcggtaag taatccatac gctgccgggg tccagcgcca gtcgttgccg ctgaagtgct
    16441 gccgcctgcg cccatttgcc ctggttttcc agtacctctg cctgctgtgc cagacggtcg
    16501 ttttgcaggc tgcgttcgat atcatcaatg ctgcgccgct ggctggcaga gagcgaggcg
    16561 ataaacgctt cagctttttc tggcgactgc tggcggtaaa tattcgccag cccgcgcacg
    16621 gcattagtgt tgccgctgtc catccgcagg gtctgctgat aatagcgttc agcggcgggg
    16681 taatctttgc gcgccatcgc cacatcgccc agccccagca ccgcatagct gtcggtgtta
    16741 tcgacattac gtgcctgctg gaacaaccgt tctgcccggt caggattatt ggctttcagc
    16801 gcagcatcac cctgctggat cgccagccag tagcggttta ctttcagcag actgttccat
    16861 ttgtcgttgt tgctgctgtg cgggtccagt gcgagggctt tttccagatt cgccactgca
    16921 ttggcgcgat cgcctttctg agaatacgcc tggcccagcg cccccagagc ttcactgtct
    16981 ttcgggttcg cccgcaccgc ctgttgtagt tcgggaatgg ctttacccgc cataccagag
    17041 tccaccgccg ctaaaccttg cgcacgagcg cggaaagcag gatcggccag ctgtttttgc
    17101 tgttctgcca gttgcgattg cgcagccgcc acgctatcgc catcactaaa gatcgagaga
    17161 tattttttca gcgccgacac actggcatca ctgacgggca tgtctttaat ctgcccgtac
    17221 cagattttag aggccccttc gcgcccggcg ttcgatttcg ccatctgttc caggacggca
    17281 aaaccttcgt cacggcgatc gctactaaac agcaatagcg ccagattgtt ttgcaggccc
    17341 gtattgcccg gtgcatccgc attgatgcgt tttaactgat taatcgcttc gccacggcga
    17401 gccggaattt tcgccaccgt actccagtac tcgacagcaa tgtcaccttc cggcggcgca
    17461 ccgttgaaca gtttgttgta actcgccaca gcttcttccg catgaccggt agtcgcctgc
    17521 aatcttgcct gttgcagtgc ctgacgacca tccggcgtgg aaagtagcat cgtagtccgc
    17581 gacgatttat acgcatttga actcggcgct aactgcgaca gccgatcgag ctgtttttgc
    17641 gcgccatcaa tatcgccctg acgtaacaaa gaacggaaac gggcggcaac gacgtccggg
    17701 ttattcggat caataagttc cagccgatat aacgactgtt gcaccagatc ttcacgatgg
    17761 gtcgcttcgc ctaaccgaac ttgctccagc aactgttgct gagcggttgg tgctgcctcg
    17821 accatcggca tgacggccag accgagggaa agcgtgaata tgtttagtgt gaatttgcgc
    17881 attcctggcc ccagtcaggt aataactcac cttttgtcga gaagcggaaa cgatgttgat
    17941 cccagtcttg tccaaacagg gtcagcacat agttgtaata ggtatcgctg ccgggaaagt
    18001 tatcggccac gcgctggcgc tgaactgcct gcgcatcgcg gttttgtaaa aaaggcagca
    18061 tggcggcaga aaaaccgacc ggtcctttac cctgcgcttt ccccgtagcc acatccactt
    18121 tttccggcgg ataaccgttt ttctcagtga atgtcgccat cggtttaaac cggttgagca
    18181 tccgcgcttt ttgcggatcg ctgtcaggca tcatgcctac ccacatgtaa acgcggatag
    18241 cgtcgtagct gctgatcaat gttttttcgg cttttagctg ccagcctttg tctttctcat
    18301 agcgcaccca gtctggcgaa aagcctttcg gggcggtttc cagcaataaa cgttgattgg
    18361 tttcgcgcag cgtggtccac ggcgcgccaa agcgggtgaa atactgcgcc agcgtcggcg
    18421 gcatgtagct ggggttaaaa cgccagctgt tatcctcagc aaaacccact ttgcccggta
    18481 acaacatgga acccagccca ggcaccgtca ccacttcctc ccgcgcgata cgttttagca
    18541 acgcgctgcc gatgtcggta taacgctgct ctttccacaa acgccccgcc tccagcaacg
    18601 accaggccat ccagacatca ccatcggagg ccgaattgct gtccagcact tcccacttac
    18661 tgttctcttt cttgccccac agccaggcgg gcaaacgttc ttttaaagaa ccctgagcga
    18721 gattgttctg cgtccagtcg agaatattat cgaaagctgc acggtcgtta gccgccaggg
    18781 caaagaacat accgtaactt tgcccttcgg aggtggtgat tttgcgcgcg tcgctggggt
    18841 cgatgacgcg cccttcctga ctgatgtaat cctttttaaa ctgctcccag gcaggcccgg
    18901 tacaggctgc ctgaacacta aaggcagcca acaacagcat cgtcacgatt ccactacgca
    18961 acacattcat cttcaattac tcgttatccg ggttaagacg acgacgacta ataattcgca
    19021 gcagacgcca cagtacccat gccagcaata tcacactgat agccgccagc accgccagca
    19081 gaatcggatg gtttgccagc gcataccaca agcgctcgaa ccacggcaga tgacctacgt
    19141 aataaacgtc gccaacacgt aggctgttga taccggactc gcggatcacc gcgaccgaac
    19201 cgaacatggt ggcgcgtttg ccgctatcgt tcaccgcatc gttaagcatt tcataaccgc
    19261 gtgggctatc tgccaacagc gcaatcacgc tgcgctggtc gttatacggc gactggaagc
    19321 caatcaccgc cgccatcgca ccggaagagg tcagcgttga ctgggtttct gccgcgcgat
    19381 cgctctcgtc cggcacaatg ccggggaacg gggtctggcg catcggtgtt ttcacccagc
    19441 tttcggtcgc ctgcaccaat aggtcgatct gcttatcgtc tttcagtttg tccgggatac
    19501 caccgatgat catgatgtcg gcatctttgc cctgaatggt gctgccatca tcggtcaccg
    19561 tcagattaat cgccgggaag cccgtctgtg cgccgataaa accaacagta ctcagcaacg
    19621 tttccatctg tgcttcgtta ggcgctttcg gcatcacggt gatggtttgc gacagatccg
    19681 ccatccggct gaatgggaag cccgcgttag caaaggcgcg tagatccggc atcgggatga
    19741 agtggtaata cttcgagaag tcgatggtgg aatcgtcacc aatcaccaca tgattctgca
    19801 ccggctggaa ggtaatacag ttatctaccg aaccgcctgg catcgggttc atatactcaa
    19861 agtcgaagcg cagctggttg gtcgcgccca gtttcagcgc cggaatagag acatctgttt
    19921 tgccatccag caaaccttgt aataccggaa tacgcagcag cagacggttc gcctcctgtt
    19981 tgctgctcag gttgaaggat tgcaggaact tgttattcag gctgatatcc atccgcgaac
    20041 tgtctttcac cggcggcatg gtgtagcggt aattaatatc catatcaatg ccggtactgc
    20101 gcatcaggta gagatccggc ggcaggttta gcgaaacgtt aatcgctgct ggctcaaggc
    20161 cgctggattg taactgttct tcataggttt tcagttcgcc aaatgtgacc ggacgatcgg
    20221 tacgtaccca gttcggcgca tcgtacggct tacgcggtag cagcggtttc acttcattca
    20281 ctaccacgct ttcaccacgg aacagaatgt taccctgagc gatacctttc gctgcctgca
    20341 acaggtcttt gtcgtcacga ccaaacacca ccagcagttt gacgtaagga ttctgcggat
    20401 ggttaatcat ctcaatcacc ggggctttta ccgccggatg atcgcgcagg aagtccggcc
    20461 gtttgtcgtt ggttgcaaag acaatggcat tgcgatccgg caattggtta tagagcaccg
    20521 ggaagttctg cccacgccag ccagaacgcg aaccaaacca cgaggcgaca atggcagatg
    20581 cttgttgcag cccaacatcc ggcgcacccg caaagaccat cggcaaggtg ttagtacggt
    20641 tatcgcgcgg gtcaaagaat ggcaccggga agtgtgacag gtcattcttc acattcaggg
    20701 tctgataggt cagatccagt ccactgctcc gcccaacatc cagccaaagc gtggtgctgg
    20761 ccgggttttc gcacacgtcc tgataatggc cgacaaactc cagccgcaca cggttgaagt
    20821 cggtaataaa cagtggatta atgggcattt gcgccagcgt ttttttaccc aactgttctt
    20881 tggtcactgg cagcacgccc atcagctcat cattgagata aacctttaac tgcgactgga
    20941 caggcagtaa cgatggcgat ggcgtgtatt cgaggttgag catcgccttc gtcaccactt
    21001 catcgctgcg cataccaaac tcaatgctgc cgttcgggtt aataccacgt agcaccatgc
    21061 tgcccggcgg cggcgcgatt tgcgcaaagg tcagcttcac atcacgcgaa ggaccgttct
    21121 gcgccacgat gggggcatca gctccctgct cgcctgacat cacttgcccc acctgcggat
    21181 tttgttccgt ctgggcggct acagctggct cagcattgat cagtggttgc gttgctggcg
    21241 tcgcctgcgt catgaaagag gggaacgcac tcatccccat agccactgca caaatccaga
    21301 atagttttct tttcatcgcg ttatcatcat tgttgagcca aagcctgatc cgatggttgt
    21361 gccgtttcgc tccgctccgg gcggcgcgga ataaacgata caacccagga aaccagagaa
    21421 gtcagcacac ggaatatgcc cttcaccgaa gaaggcgcaa actccgccag atggcggtag
    21481 ccacggaagc cgagcttcag aatatccagc agactttcca gcggcttatc ttccgggtag
    21541 ctgtcctgcc agagcgccca tgtatccgca cgggcaaacg tacactgcac aaaatcgata
    21601 tgttgctggg tggtgagcgg cattaatttc agcccaactt cattacccat cacgcgcgcc
    21661 acctgggtcg ggaagacgta ttcctgctga ccgcgtttaa gcaacagatt cactttctgc
    21721 ccttccagaa tctgcgcctg accgttgatc ttgatcccca aaccaccgtc ggagaaatcc
    21781 tgaacggtac acgagaagag gtgaccatct tcgcgggcaa ttgccgcggg catcgtcatc
    21841 tccacgcggt gcgatcggcg tacctgtttg ctttctaccg ataccgcaac tgcgccgcca
    21901 agaacaatca ggttgtagaa cacccacacc atactgacga ccacggtgag catctcggtt
    21961 ggcgggccat agaagtagcg ccagatgcct accgcaacgc ccaccaggtt gagtagaaca
    22021 aggaagatgt agggccgcga gatcacccag tcgacgtact cttcttccac cagtccacct
    22081 ttggcggtga cgttaaattt gcctttgtgc gggttaatca gcgccaccag cgtcggtggt
    22141 gcgatatacc acgccagcac cgtttcgtag atttcactcc agaaagagtg gcgatggatc
    22201 ttcgagttgg tcaggctggc atggatcata tgcggcagca cgaatagggc gatcatcaac
    22261 gctggcgcat agatgatgta ggcatgaagc agcaggaacg ccagcggcgc agtcaggaag
    22321 atcagccgtg gaatgcccga caagaagtgg aacatggcgt tgacgtagca cagccgctga
    22381 gcaaacttca gtcctttacc ggtgagcggg ttatcgagac ggaagatttg taccatcccg
    22441 cgcgcccagc gaatacgctg accgatatgc gccgacagac tttcggtcgc cagccccgcc
    22501 gcctgcggaa tacgcatata cgcggaggta tagccacgac ggtgcaaacg cagagaagta
    22561 tgcgcatctt cagtcacggt ttcgacagca atgccgccaa tttcatccag cggcttacga
    22621 cgaatcaccg cacaggaacc gcagaagaaa gtggcgtccc acatatcgtt gccatcctgc
    22681 accagaccat agaacagcgt gccttcgttc ggcgttttac gaaaacgtcc caggttgcgt
    22741 tcaaacgggt ccggcgagaa gaagtggtgt ggagtctgca tcatcgccag ctgtttctct
    22801 ttcaggaacc agcccatggt catttgcagg aacgatcgcg ttggtacgtg gtcgcagtcg
    22861 aaaatcgaca cgaactcgcc tttggcatat ttcagcgcat tgttgatgtt gcccgctttc
    22921 gcatgttcat gagtggtgcg ggcgatatat ttcaccccca cgttttgcgc aaactggcga
    22981 aactcttccc tgccgccgtc atcgaggatc cagatgttca gtttgtcttt cggccagtcg
    23041 atacccagcg aggcgtaaat ggtatttttc accacattga gatcttcgtt gtaagtcggg
    23101 acaaagatat ccactgacgg ccacagcgac atatccttcg gcaatggcac cggctgacga
    23161 ttcagcggcc atactacctg gaagtagccg agcaccagca caatccacgc gtacgtttca
    23221 gcgaagagca gaataagccc gcacaccagg ctgaccggat cgtcccagtt cagcgtagag
    23281 gtgtaacgcc accagatata acggcaagaa acggtcagcg acagcacaat caacattagc
    23341 gccgagaagc gccccggcat ccgccgtacg atcagcgcta ccccccacag cagcatcagg
    23401 aagataaact gcgccagcgg gttaaacggc tgagtaacgc agattaacgc cagaatcagc
    23461 gagaaggtga cgatgatacc gaggatcaac cgccgcgccc cggcactcaa atgaccgagc
    23521 tcttttttct catccagatg ctgtgtttta tggctaacgc gctcaggcag ctcgttcatc
    23581 cattgatggt aacgtccacg aatattttgc agacctgaaa atgcccgctg cgcggtttcg
    23641 gcgtttcttt gcgcgatgca ccgatcaata accagcatgt ttgaatgaga taacggaccg
    23701 ggtccagcgg acgcgaggcg ttgatatgcg tatacaggtt tttatgttct gcgcgaatac
    23761 gctgccagcg cgggtgctcc agcggaataa aaatccaggc caggatcatc cagaaacagc
    23821 cgagcgtcgc gctgaaagcc gacgcaccgt gacgacgata atcgcgataa cgcccgataa
    23881 gccgcgcgtt gaccggcggg ataagcaacc accgggtcag gatactcatg atgcactccc
    23941 gactggcgtt ttcagcccgg agtagttcaa caggcaccag ttcgccagcg tcagtatctc
    24001 ttcagccgcc agcgcatcac tgcgatattc acctactggt tgcttagccg ccaggcattc
    24061 agccatcgct tcatcacgat gaatgagcat cggcagtaat cggcgctggc tttgcaacca
    24121 aagctggtaa atatcgtcct gaacctgact gccaatacgg aagtcattaa tcaaaatatg
    24181 tgcgccatcc ggcagcgctt gctgatgcag tcggatatgg cagttggcat ccacattgac
    24241 gattgccagc gagtgctcgc acaaactcag cagctggtgg gttatctgcg aggcatcacg
    24301 cggtaagtcg attaaaatcc actggtaacg cccgttggct tttagttgct gtaagccgga
    24361 gcaaatatcg ctcagccggg tttgccagtg ctgtggattt tcttgttctt caatggataa
    24421 ctgaccaaaa ggcagcaaat cgagctgcga ggtgtagcgc aacccagcgt cacgccagtc
    24481 ctggccatcc agcatcgctc tggcccagcc ctgacggtgg gtaaaatcaa cattaaatga
    24541 caggcgcaac aagttgtccg ggcaggcatc gaccaccagg acattttctc ccaacatttg
    24601 taatgaccag gctaatgcgg cggtgatggt tgttgtcccc acgcctcccc gcaccccctg
    24661 caatcccagt acggccattc accaatcccc tacttttgtt gcgcaaactc tgccagcaac
    24721 ggccagcgtt ttaatgccgc ggccaactgt tcgcgttggg aaatatcggc ataatcaata
    24781 tcaggcagtg aaaatgcctg ctttaacgcc acaatatcat tctggaagat atagcctatc
    24841 gcgggatcag gcagagtatc tggttcattg ttattcattt tgcttgatcc ctatgaaata
    24901 accgtaccgt tcatgagaca attctaaccg tgagcatttc gccggtttat tgtttacggc
    24961 gagacgggaa tttgctcact ttttgacttt ccgttacgca acagaaaaag ttaaaaacga
    25021 aaagactaaa atcacacgtc ttgtgacctt ctaaattgat tcacatgcta aatctgataa
    25081 gttttaattt caatggtagg tttatttctt agctttcgct agtaaactga taatcagaca
    25141 aaatggtgac ataagggaca ttgtggaccc tgtattctct atcggtatct catcattatg
    25201 ggatgagctg cgacatatgc cagcaggcgg cgtctggtgg tttaacgtcg atcgccatga
    25261 agatgctatc agtctggcga atcaaacaat tgcatcccag gctgaaaccg cacacgtcgc
    25321 ggtcattagc atggacagcg atccggcgaa aatctttcaa ttagatgatt ctcaagggcc
    25381 ggaaaaaata aaattatttt caatgctaaa tcatgaaaaa ggtctatact atttggcccg
    25441 tgatttgcag tgttctattg atccccataa ttaccttttt attcttgttt gcgcaaataa
    25501 cgcatggcaa aacattcctg ccgagcggct tcgctcatgg ttggataaaa tgaataaatg
    25561 gagcaggtta aaccattgtt cgcttttggt aattaatccc ggaaataata acgataaaca
    25621 attttcattg ttgcttgagg aataccgttc ac
//
LOCUS       DADLSB010000044        25500 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.044, whole genome shotgun sequence.
ACCESSION   DADLSB010000044 DADLSB010000000
VERSION     DADLSB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.044"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..531
                     /locus_tag="JN331_001783"
     CDS             <1..531
                     /locus_tag="JN331_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7259947.1"
                     /translation="EKLQQAVSKLHIGDGLEKGVTIGPLIDEKAVAKVEEYIADALEK
                     GARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLAPLFRFKDEADVIAQAN
                     DTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVAPFGGIKASGLGREGSK
                     YGIEDYLEIKYMCIGL"
     gene            545..1825
                     /gene="gabT"
                     /locus_tag="JN331_001784"
     CDS             545..1825
                     /gene="gabT"
                     /locus_tag="JN331_001784"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HAY7259948.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTRHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            2158..3498
                     /gene="gabP"
                     /locus_tag="JN331_001785"
     CDS             2158..3498
                     /gene="gabP"
                     /locus_tag="JN331_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAY7259949.1"
                     /translation="MLSIAGVIGASLFVGSSVAIAEAGPAVLLAYLFAGLLVVMIMRM
                     LAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFWVLVIPLEANIAAMILHSWVP
                     GIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKVIAILAFIFLGAVAISGIYPY
                     AEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAEIVTIAAAESDTPEKHIVRAT
                     NSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSVLELLNIPHAKLIMDCVILLS
                     VTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYY
                     APAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIG
                     FITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            3519..4181
                     /gene="csiR"
                     /locus_tag="JN331_001786"
     CDS             3519..4181
                     /gene="csiR"
                     /locus_tag="JN331_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAY7259950.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(4182..4631)
                     /gene="kbp"
                     /locus_tag="JN331_001787"
     CDS             complement(4182..4631)
                     /gene="kbp"
                     /locus_tag="JN331_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY7259951.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVLEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(4715..4873)
                     /locus_tag="JN331_001788"
     CDS             complement(4715..4873)
                     /locus_tag="JN331_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY7259952.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            5056..5355
                     /locus_tag="JN331_001789"
     CDS             5056..5355
                     /locus_tag="JN331_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7259953.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            5365..5889
                     /gene="ygaP"
                     /locus_tag="JN331_001790"
     CDS             5365..5889
                     /gene="ygaP"
                     /locus_tag="JN331_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY7259954.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(5936..6340)
                     /gene="stpA"
                     /locus_tag="JN331_001791"
     CDS             complement(5936..6340)
                     /gene="stpA"
                     /locus_tag="JN331_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY7259955.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKFLDDFLI"
     gene            7008..7457
                     /gene="alaE"
                     /locus_tag="JN331_001792"
     CDS             7008..7457
                     /gene="alaE"
                     /locus_tag="JN331_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY7259956.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(7494..7838)
                     /locus_tag="JN331_001793"
     CDS             complement(7494..7838)
                     /locus_tag="JN331_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY7259957.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            7990..8319
                     /locus_tag="JN331_001794"
     CDS             7990..8319
                     /locus_tag="JN331_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7259958.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            8567..8812
                     /gene="nrdH"
                     /locus_tag="JN331_001795"
     CDS             8567..8812
                     /gene="nrdH"
                     /locus_tag="JN331_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY7259959.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPQAASA"
     gene            8809..9219
                     /gene="nrdI"
                     /locus_tag="JN331_001796"
     CDS             8809..9219
                     /gene="nrdI"
                     /locus_tag="JN331_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY7259960.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            9192..11336
                     /gene="nrdE"
                     /locus_tag="JN331_001797"
     CDS             9192..11336
                     /gene="nrdE"
                     /locus_tag="JN331_001797"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY7259961.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            11346..12305
                     /gene="nrdF"
                     /locus_tag="JN331_001798"
     CDS             11346..12305
                     /gene="nrdF"
                     /locus_tag="JN331_001798"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY7259962.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            12660..13862
                     /locus_tag="JN331_001799"
     CDS             12660..13862
                     /locus_tag="JN331_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY7259963.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            13855..14919
                     /gene="proW"
                     /locus_tag="JN331_001800"
     CDS             13855..14919
                     /gene="proW"
                     /locus_tag="JN331_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY7259964.1"
                     /translation="MADQNNPWDTTPAAGSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            14977..15969
                     /gene="proX"
                     /locus_tag="JN331_001801"
     CDS             14977..15969
                     /gene="proX"
                     /locus_tag="JN331_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY7259965.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KSGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            16161..17345
                     /locus_tag="JN331_001802"
     CDS             16161..17345
                     /locus_tag="JN331_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7259966.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCMTFANFSILWTSMAFLLAAPPFNYSDGDIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGATGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            17469..18206
                     /locus_tag="JN331_001803"
     CDS             17469..18206
                     /locus_tag="JN331_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY7259967.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            18196..18531
                     /gene="ygaH"
                     /locus_tag="JN331_001804"
     CDS             18196..18531
                     /gene="ygaH"
                     /locus_tag="JN331_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY7259968.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(18550..18651)
                     /locus_tag="JN331_001805"
     CDS             complement(18550..18651)
                     /locus_tag="JN331_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7259969.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            18622..19152
                     /gene="emrR"
                     /locus_tag="JN331_001806"
     CDS             18622..19152
                     /gene="emrR"
                     /locus_tag="JN331_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY7259970.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            19279..20451
                     /gene="emrA"
                     /locus_tag="JN331_001807"
                     /pseudo
     CDS             19279..20451
                     /gene="emrA"
                     /locus_tag="JN331_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            20468..22006
                     /gene="emrB"
                     /locus_tag="JN331_001808"
     CDS             20468..22006
                     /gene="emrB"
                     /locus_tag="JN331_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY7259971.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRLAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(22070..22585)
                     /gene="luxS"
                     /locus_tag="JN331_001809"
     CDS             complement(22070..22585)
                     /gene="luxS"
                     /locus_tag="JN331_001809"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY7259972.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(22735..24291)
                     /gene="gshA"
                     /locus_tag="JN331_001810"
     CDS             complement(22735..24291)
                     /gene="gshA"
                     /locus_tag="JN331_001810"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7259973.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(24364..24792)
                     /locus_tag="JN331_001811"
     CDS             complement(24364..24792)
                     /locus_tag="JN331_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7259974.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(24789..25355)
                     /gene="yqaB"
                     /locus_tag="JN331_001812"
     CDS             complement(24789..25355)
                     /gene="yqaB"
                     /locus_tag="JN331_001812"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY7259975.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         6064 a         6524 c         6527 g         6385 t
ORIGIN      
        1 gaaaaattac agcaggcggt gagcaaactg cacatcggcg acgggctgga gaaaggcgtc
       61 accatcgggc cgctgatcga tgaaaaagcg gtagcaaaag tggaagagta tattgccgat
      121 gcgctggaga aaggtgcgcg tgtggtttgc ggcggtaaag cgcacgaacg cggcggcaac
      181 ttcttccagc caaccattct ggtggacgtt ccggccaacg ccaaagtgtc gaaagaagag
      241 acgttcggcc ccctcgcccc actgtttcgt tttaaagatg aagccgatgt gattgcacaa
      301 gccaatgaca ccgagtttgg tcttgccgcc tatttctacg cccgtgattt aagccgcgtc
      361 ttccgcgtcg gcgaggcgct ggagtacggc atcgtcggca tcaataccgg cattatttcc
      421 aatgaagtgg ccccattcgg cggcatcaaa gcctcgggtc tgggtcgtga aggttcgaag
      481 tatggcatcg aagattactt agaaatcaaa tatatgtgca tcggtcttta actggagaat
      541 gcgaatgaac agcaataaag agttaatgca gcgccgcagt caggcgattc cccgtggcgt
      601 tgggcaaatt cacccgattt tcgctgaccg cgcggaaaac tgccgggtgt gggacgttga
      661 aggccgtgag tatcttgatt tcgcgggcgg gattgcggtg ctcaatacca ggcacctgca
      721 tccgaaggtg gtggccgcgg tggaagcgca gttgaaaaaa ctgtcgcaca cctgcttcca
      781 ggtgctggct tacgagccgt atctggagct gtgcgagatt atgaatcaga aggtgccggg
      841 cgatttcgcc aagaaaacgc tgctggttac gaccggttcc gaagcggtgg aaaacgcggt
      901 aaaaatcgcc cgcgccgcca ccaaacgtag cggcaccatc gcttttagcg gcgcgtatca
      961 cgggcgcacg cattacacgc tggcgctgac cggcaaggtg aatccgtact ctgcgggcat
     1021 ggggctgatg ccgggtcatg tttatcgcgc gctttatcct tgcccgctgc acggcataag
     1081 cgaggatgac gctatcgcca gcatccaccg gatcttcaaa aatgatgccg cgccggaaga
     1141 tatcgccgcc atcatgattg agccggttca gggcgaaggc ggtttctacg ccgcgtcgcc
     1201 agcctttatg cagcgtttac gcgctctgtg tgacgagcac gggatcatgc tgattgccga
     1261 tgaagtgcag agcggcgcgg ggcgtaccgg cacgctgttt gcgatggagc agatgggcgt
     1321 ggcgccggat cttaccacct ttgcgaaatc gatcgcaggc ggtttcccgc tggcgggcgt
     1381 caccgggcgc gcggaagtga tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc
     1441 cggtaacccg attgcctgcg tggctgcgct ggaagtgttg aaggtgttcg agcaggaaaa
     1501 tctgctgcaa aaagccaacg atctggggca gaagctaaaa gacggattgt tggcgatagc
     1561 cgaaaaacac ccggagatcg gcgacgtacg cgggctggga gcgatgattg ccatcgagct
     1621 gtttgaagat ggcgatcaca gcaaaccgga cgcaaaactc accgccgaga tcgtggcacg
     1681 cgcccgcgat aagggcctga ttcttctctc ctgcggcccg tattacaacg tgctgcgcat
     1741 ccttgtaccg ctcaccattg aagacgctca gatccgtcag ggtctggaga ttatcggtca
     1801 gtgttttgat gaggcgaagc agtagcaccg cccccatgca ggaggcgacg ctgcgcgtct
     1861 tgtccggcct acgagaatcc aagtcggata aggcgtttac gccgcatccg acaatctgta
     1921 cgtgaacagg aataaatcta tgtcggccgg ataaggcgga gccgctctcc ggcaaaaaga
     1981 atcaataaca attaaacgcg tgacccgacg cgggaaatgt cggggcgctc tcccaagtga
     2041 cacactttcg agaggattca ggatggggca atcatcgcaa ccacacgagt taggcggcgg
     2101 gctgaaatca cgtcacgtca cgtcacgtca cgtcacgtca cgtcacgtca cgtcaccatg
     2161 ttgtctattg ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt cgccatcgcc
     2221 gaagcgggcc cggcggtatt actggcctat ctgttcgccg gattactggt ggttatgatt
     2281 atgcggatgc tggcggaaat ggcagttgcc acgcccgata ccggttcgtt ttccacctat
     2341 gccgataaag ccattggccg ctgggcgggc tataccatcg gctggttgta ctggtggttt
     2401 tgggtactgg ttatcccact ggaagccaac atcgccgcca tgatcctgca ctcgtgggtt
     2461 ccaggcattc ccatctggtt attttccctc gtcattaccc tcgccttaac tggcagtaat
     2521 ttattaagcg ttaaaaacta cggcgaattt gagttctggc tggcgctgtg caaagtcatc
     2581 gctatcctgg cctttatttt ccttggtgca gtcgcaatta gcggtattta cccttatgcc
     2641 gaagtgagcg ggatctcaag attgtgggat agcggcggct ttatgcccaa cggtttcggt
     2701 gcggtattaa gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga aattgtcacc
     2761 attgccgccg cggaatccga cacgccggaa aaacatattg tccgcgccac caactcggtt
     2821 atctggcgta tttctatctt ctatttgtgc tctatttttg tcgtcgtggc attaataccg
     2881 tggaatatgc cggggctgaa agccgttgga tcttatcgct cggttctgga attgctcaat
     2941 attccccatg cgaaattaat catggactgt gtgatattac tttccgtaac cagttgcctg
     3001 aactcggccc tgtataccgc ctcaaggatg ctctactcct taagccgtcg cggtgatgct
     3061 cccgcggtaa tgggtaaaat caaccgcagt aaaaccccgt acgtggcggt gttactctcc
     3121 accggcgcgg cattcttaac ggtggtggtg aactattacg cgcctgcgaa ggtatttaaa
     3181 tttctgatcg acagctccgg cgctatcgcc ctgctggttt atttagtcat cgccgtttca
     3241 cagttgcgga tgcgcaaaat tctgcgagca gaaggaagcg aaattcgctt gcgaatgtgg
     3301 ctttatccct ggctcacctg gctggtcatc ggctttatta cctttgtgtt ggtagtgatg
     3361 ctattccgtc cggcgcaaca gttagaagtg atctccaccg gcttattagc gatagggatt
     3421 atctgtaccg tgccaattat ggctcgctgg aaaaagctgg tattgtggca aaaaacaccc
     3481 gttcataata cgcgctgata atgatcagga gtcacaccat gaccattacg tctctggatg
     3541 gctatcgctg gctgaagaac gatattattc gcggtaattt tcaaccggat gaaaaattac
     3601 ggatgagttt actgacatcg cgttatgcac ttggcgttgg gccgttgcgg gaagcacttt
     3661 ctcaactggt ggcggaacgg ctggtcacgg tggtgaatca aaaagggtat cgggtggcgt
     3721 ctatgtcaga gcaggagctg ctcgatattt tcgacgcccg cgccaatatg gaagcgatgc
     3781 tggtcagtct ggcgattgcc cgcggtggcg atgaatggga ggcagacgtt ctcgcaaaag
     3841 cgcatctgct cagtaagctg gaagcctgtg acgccagcga gaaaatgctt gatgagtggg
     3901 atctgcgtca tcaggcgttt catacagcaa ttgtggcggg ctgtggttct cactatttgc
     3961 tgcaaatgcg tgaacggttg tttgatctgg cggcgcgtta tcgatttatc tggctgcggc
     4021 gaacggtgct ttcggtggaa atgctggagg ataaacacga tcagcaccag accctgactg
     4081 cggcggtact ggcgcgagat acagcgcgcg ccagtgagtt aatgcgccag catttactga
     4141 cgccaattcc cattatccag caggcgatgg ctggcaatta actactcttc cggaatacgc
     4201 aacacttgcc ccggataaat tttatccggg ctttttagca tcggtttatt cgcttcgaag
     4261 attttattgt acagattagc gttaccgtag acctgtttgg aaatggcact cagagtgtcg
     4321 ccagacttaa cggtataaaa ctggctggca gtggctggtg tcgccgtttt cacctgatca
     4381 tcaacactgg caataccgga aatattcccc accgcaacaa ggattttctc cttcgcctcc
     4441 tgactcaggc cgtcaccagt gaccgtcgct ttgccgtcgg caatttgaat gttcacttta
     4501 tcggcatcag gtataccggt tttgctcaga tgctccagca ccttcttcgc ctgatcgtct
     4561 ttatcgtgct gacctgtaac cgcgtcccag agtttttctc cggcatcttt cacaaaattg
     4621 aacagaccca tagctacctc attcgttaac ggaaacactc agaaagtgta gcagaggaac
     4681 gcggcgcgtt aacgccgcgg gggtgagcac aggtttaatc gcgggtttgc acccagaacg
     4741 cgtgaatcaa accggggata tacccaagca gtgtcagcag aatattaata atgaacgccc
     4801 aaccgaaccc tttaccgagc agcacgccga gcggcggcag aatgatggtg atgacgattc
     4861 tccagaaacc catatgtact ccctataaga aaattactca ttgtttaaaa agagatttta
     4921 tctcttaagc gtagtaattt tagcggagcc tgccagtttc agcttacgct tttactctca
     4981 tttacgctgc tgaggctggt atatgttgca tttttagttt agagtttact taatttagaa
     5041 aatacttaaa taaatatgac tgaactcgcg caattacagg ccagtaccga acaggcagcg
     5101 gccttattga aagcaatgag ccaccctaaa cggttgctga ttctgtgcat gcttagcggt
     5161 tcccccggca ccagcgcggg agagctgacg cgcattaccg gactgagcgc ctctgcgaca
     5221 tcacagcatc tcgctcgtat gcgggacgaa gggcttatca acagccaacg ggatgcccaa
     5281 cgcattctat attccattaa aaatgaggcg gtaaatgcca ttatcgccac cctgaaaaat
     5341 gtctattgtc cgtaaggagt cactatggct ttgacaacca tttcgccgca tgatgcacaa
     5401 gaattaatcg cacgcggcgc aaagttaatc gatattcgtg acgctgatga atatcttcgt
     5461 gaacatattc ctgaagcaga tctggctcca ttatccgtgc tggaacagtc aggtcttcca
     5521 cctaaattac gtcgcgagca aattatattc cactgccagg caggtaaacg caccagtaat
     5581 aacgccgata aattagcagc gattgccgcc cccgcagaaa tatttttact cgaagatggg
     5641 attgatggct ggaaaagagc tggattgcca gtagcggtaa ataaatctca acccttgccg
     5701 ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat taatcggcgt tgtactgggt
     5761 tataccgtaa atagcggttt cttcttatta agtggctttg ttggtgccgg gttactgttt
     5821 gcaggaatca gcggtttttg tggaatggca aggttgttag ataagatgcc gtggaaccaa
     5881 cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac atccggcctc agtaattaga
     5941 tcaggaaatc gtcgagaaat ttaccttctg ccagcgcctg agcaattggc ttcggtgtac
     6001 ggccctgacc ggtccaggtt ttagtttcac cgttaacatc agtgaattta tatttcgccg
     6061 gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc agagctatta cccaataact
     6121 cttccgggtt aattccgtca gctttcatca gctccagcca ggtgctaatt ttttcctgac
     6181 gctcagccag ttcacgctgc tgctgttctt cttcttcacg tctttcttta gtgacaaccc
     6241 tgaatttttc gagcatttct tcaagaacgt caatggagaa ttcgcgagcc atcgcacgga
     6301 gggtgcgaat gttatttaaa ctttgtaaca ttacggacat aataaagaaa accttttaac
     6361 gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat cacttattat atttccacaa
     6421 aatttaatct atttacagtc ctgcgcgaga ttatttcagc tgttattttc tgaaactctc
     6481 agaataattt caagattata tattagccat tttgatgagt aatttaggtt attggtgaac
     6541 agagtggcgc tctctgctcg ctaactccgc aagcctgaca aaaccgaagt gaataaaaat
     6601 gcaatataac agaataaaag aaaacacccc tcactggtgc tttctgctgt taatgtacat
     6661 tttctggcat caaaaatgct gtaaatgacc cggtcacgac aaacaccgaa ttctctacgc
     6721 ttatagcgtt acctcacccc caaactttca ttagtgaaat taaaggcaag gccatatcca
     6781 attgatttaa ttgaaaataa atcatatcag catgtaaaaa tccacaatgt acaaaaaacg
     6841 agccgttacg gaatatttta tctacaaaaa ctgactaaat aaaaattttt cactaattga
     6901 ttagtcatag ccagcgatat acgctatgcg aaaatgcaga ttgcaatgag atccactgct
     6961 ttcatctcca ttaacatccc attacgcttt tattaaggag cattagcatg ttctcaccgc
     7021 agtcacgctt gcgtcatgca gttgcagata cgttcgcgat ggttgtttac tgttctgtcg
     7081 tgaacatgtg tattgaagtt ttcctctccg gaatgagctt cgaacagtct ttttattcca
     7141 gattggtagc gattccggtg aacatcttaa ttgcatggcc atacggtatg taccgcgatc
     7201 tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg gataaaaaat ctggctgata
     7261 tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc gatcttgtta gtggtgggcg
     7321 cagactggca tcagattatg gcggcggtca gttcaaacat cgttgtttcg atgttgatgg
     7381 gggcggttta tggctacttc ctcgattatt gccgccgact gtttaaagtc agccgttacc
     7441 agcaggtaaa agcctgactc ttcttttcgc gactggcatc gccagtcgcg tctttaactg
     7501 gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa cacgcgaact aaagccatgc
     7561 gggataccgt aatgctcgtg tcgctcgcct gctaaataga gcggaaattg ctggtaatga
     7621 tcgcaagttt taatatcgga agcgggttca gccagtgttg aactgcgttc ttttaatgtt
     7681 ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc gattaacata gacataattt
     7741 tccccacggc ggtaaccata cgctttctgg gttaccacat cgacagtaaa accgactttt
     7801 tcaagtacgc gcgccacctc gtctggtcgt aaatacatat ttgatcctcg ttattttcaa
     7861 ccgcgggcct accttacctg attgcgcatt cacaacgctt tcagaaaagt ccataaagcc
     7921 gcgatgaacc tcagttaaga aaattatggt ctacactgaa aattacatcg aattctaatg
     7981 ggggatcata tgtttaaccg accgaaccgc aacgacgttg atgatggcgt gcaggatatc
     8041 cagaatgatg tcaatcaatt agctgacagc ctcgaatctg tattgaagtc ctggggcagc
     8101 gacgccaaag gggaagctga agccgcacgc agtaaagctc aggcgttgct gaaagaaacc
     8161 agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg cgcgcgatgc cgttggctgc
     8221 gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg gtacagcagc tgcggtaggt
     8281 atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt gcaaaaatga taataaatac
     8341 gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt gcctggtgaa tcacaaaaat
     8401 ccccctcccc cgtcacgctc atatctaggg taatttcgat cactatttgc tatatattgt
     8461 gtggttgaat cttttttcaa ctacatctag tatctctgta tcaacagaga gacaacccga
     8521 cgcgtatcat cgcgccgtat cttcatttta aacggaaata cgaatcatgc gcataactat
     8581 ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa cgggcgatgg aaaaccgggg
     8641 ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa gcagcggaag cgttgcgtgc
     8701 tcagggcttt cgtcagttgc cggtagtgat tgctggcgat cttagctggt ctggtttccg
     8761 tccggacatg attaaccgtc tgcatccagc gccacaagcg gccagtgcat gagccagctc
     8821 gtctacttct ccagcagctc cgaaaacacg cagcgtttta tcgaacgttt aggtctgccc
     8881 gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg tagacgagcc ttacatcctg
     8941 atcgtgccct cttacggcgg cggcggtacg gctggcgcgg tgccacgaca ggtaattcgc
     9001 tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg ttattgcttc tggtaatcgc
     9061 aactttggtg aggcgtatgg ccgcgccgga gatgtgattg cccggaaatg cggcgtgccg
     9121 tggctgtacc gttttgaact catgggtacg caaagcgata tcgaaaacgt tcgtaaagga
     9181 gtaaccgaat tttggcaacg acaaccgcag aatgcctgac gcaggaaacg atggattacc
     9241 acgcgctgaa tgcgatgctt aatctttacg atagcgcagg tcgcattcag ttcgataaag
     9301 accgccaggc cgttgacgcc tttattgcga cgcatgtgcg tccgaacagt gtgaccttca
     9361 gtagccagca gcagcgcctg aactggctgg tcaacgaagg ttactatgat gaaagcgttc
     9421 ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc ccacgcacac accagcggtt
     9481 ttcgtttcca gacattcctc ggggcatgga agttttacac cagctatacg ttgaagacat
     9541 tcgacggtaa acgttatctg gaagattttg ccgatcgagt cacgatggtg gcgctgacgc
     9601 tggcacaagg cgatgagacg ctggcgttgc aactgaccga tgaaatgctg tcaggacgct
     9661 ttcagccagc cacgccaaca ttcctcaact gcggtaagca gcagcgcggc gaactggttt
     9721 cctgtttttt gctgcgtatt gaagacaata tggagtcgat tggtcgggcg gtaaattccg
     9781 cactgcagct gtcgaaacgc ggcggcggcg tagcattttt gctgtcgaat ctgcgggaag
     9841 cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg cgtaattccg gtgatgaaaa
     9901 tgttggaaga cgcattttcc tatgccaacc aactcggcgc gcgtcagggg gcaggtgcag
     9961 tctatttaca tgctcatcat cccgatattc tgcgttttct cgacacgaaa cgggaaaatg
    10021 ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt ggtgatcccg gatatcactt
    10081 tccatctggc aaaagagaat gcgcagatgg cgctgttttc gccctatgac gttgagcgag
    10141 tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca ctatgacgaa ctggttgccg
    10201 atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt cttccagcga ctggcagaaa
    10261 tccagtttga gtccggctat ccctacatca tgtatgaaga cacagtaaac cgtgctaacc
    10321 ctatcgccgg gcgcataaat atgagtaatc tctgctcaga aattttgcag gttaacagcg
    10381 cctcagagta tgacgagaat ctcgactatg cccgcacagg ccatgatatt tcctgcaatt
    10441 taggttcgtt gaatattgcg cacaccatgg attcccccga tttttcccgc acggtagaga
    10501 ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca tatccgcagc gtgccgtcca
    10561 tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg gcagatgaat ttacatggtt
    10621 atctggcgag ggaaggcatc gcttatggtt cgccggaagc cctggatttc accaatctct
    10681 atttctatac catcacctgg cacgcactgc gtacctcgat gttgctggcg cgcgaacgcg
    10741 gtgaaacctt cgccgggttc aaacagtcac gctatgccag tggtgaatat ttcagccaat
    10801 atctgcaagg taactggcag ccgaaaacgg cgaaagttgg cgaactgttt gcccgcagcg
    10861 gtattacgtt acctacccgt gagatgtggg cgcaactgcg cgacgacgtg atgcgctacg
    10921 gcatatacaa ccagaatctc caggcggtgc cgccaaccgg gtctatctct tatatcaacc
    10981 atgctacgtc gagtattcat ccgattgtgg cgaaagtaga gatacgcaaa gagggcaaaa
    11041 caggacgcgt ttactaccct gccccgttta tgactaacga aaatctggcg ctgtatcagg
    11101 acgcttacga aattggcgca gaaaagatca tcgacaccta cgcggaagcg actcgccatg
    11161 tcgatcaggg gctgtcgctg acgctgtttt tccccgatac cgccaccact cgcgatatca
    11221 acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac gctctattac atccgcctgc
    11281 gtcagatggc gctggaaggc actgaaattg aaggctgcgt ctcctgtgca ctttaaggaa
    11341 tatctatgaa actctcacgt atcagcgcca tcaactggaa caagatatct gacgataaag
    11401 atctggaggt gtggaatcgc ctgaccagca atttctggct accagaaaag gtgccgctgt
    11461 cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga acaacaactg acgatgcgcg
    11521 tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt tatcggcgcg ccttcgctga
    11581 tgcccgatgc gctcacgcct catgaagagg cggtattatc gaatatcagc tttatggaag
    11641 cggttcatgc ccgctcttac agttcgattt tctcgacgct gtgccagacc aaagatgtcg
    11701 atgccgccta cgcctggagt gaagaaaacg caccgttgca gcgaaaagct cagattattc
    11761 agcaacatta tcgcggtgat gatccgctga aaaagaaaat cgccagtgtg tttcttgaat
    11821 cttttttgtt ctattccggt ttctggctgc cgatgtattt ttccagccgc ggaaagctga
    11881 ccaataccgc ggacctgatc cgtctgatta tccgcgatga agcagtccac ggttactaca
    11941 taggctataa atatcagaaa aacatggaaa agatatctct gggacaacgt gaagagttga
    12001 agagtttcgc cttcgatttg ttgctggaac tctacgacaa cgagttgcaa tacaccgatg
    12061 agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc gtttctctgt tacaacacca
    12121 ataaggcttt gatgaatctg ggctacgaac cgttatttcc cgcagaaatg gcggaagtga
    12181 atccggcaat cctcgccgcg ctttcgccga atgccgatga aaatcacgat ttcttttccg
    12241 gttcaggctc ctcttatgtg atggggaaag cggttgaaac agaagatgaa gactggaatt
    12301 tctgagggtg ttattttcaa aaatatcact acccgcagca gggaaataat tcccgccaaa
    12361 tagcttttta tcacgcaaat attctgtagt gatctacact gatactctgt tgcattattc
    12421 gcctgaaact acaatattca ggcgtttttt cgctatctat gacaaaaaat atcaactttt
    12481 ctcgatttgc tctcagccct tatatcacgg aaaattccgg cgatttgctc gcatcaatat
    12541 tcatgccaca tttgccctca ggggttgcct cagattctca gtatgttagg gtagaaaaaa
    12601 gtgactattt ccattgggta atatatcgac ataaacaaat aaaggaatca ttctattgca
    12661 tggccattaa attagaaatt aaaaatcttt ataaaatatt tggtgagcat ccacagcgag
    12721 cgttcaaata tatcgaacaa ggactttcaa aagaacaaat tctggaaaaa actgggctat
    12781 cacttggcgt aaaagacgcc agtctggcca ttgaagaagg cgagatattt gtcatcatgg
    12841 gattatccgg ctcgggtaaa tccacaatgg tacgccttct caatcgcctg attgaaccca
    12901 cccgcgggca agtgctgatt gatggtgtgg atattgccaa aatatccgac gccgaactcc
    12961 gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc ctttgcctta atgccgcata
    13021 tgaccgtgct ggacaatact gcgttcggta tggaattggc cggaattaat gccgaagaac
    13081 gccgggaaaa agcccttgat gcactgcgtc aggtcgggct ggaaaattat gcccacagct
    13141 acccggatga actctctggc gggatgcgtc aacgtgtggg attagcccgc gcgttagcga
    13201 ttaatccgga tatattatta atggacgaag ccttctcggc gctcgatcca ttaattcgca
    13261 ccgagatgca ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc attgtcttta
    13321 tttcccacga tcttgatgaa gccatgcgta ttggcgaccg aattgccatt atgcaaaatg
    13381 gtgaagtggt acaggtcggc acaccggatg aaattctcaa taatccggcg aatgattatg
    13441 tccgtacctt cttccgtggc gttgatatta gtcaggtgtt cagtgcgaaa gatattgccc
    13501 gccggacacc gaatggctta attcgtaaaa ctcctggctt cggcccacgt tcggcactga
    13561 aattattgca ggatgaagat cgcgaatatg gctacgttat cgaacgcggt aataagtttg
    13621 tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa ggtcttgatg
    13681 cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc gagttgctct
    13741 ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa cagtatgtcg
    13801 gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta aataatggct
    13861 gatcaaaata atccgtggga taccacgcca gcggcgggca gtgccgcgca atccgcagac
    13921 gcctggggta caccgacgac tgcaccggct gacggcggtg gtgctgactg gctgaccagt
    13981 acgcctgcgc caaacgtcga gcattttaat attctcgatc cgttccataa aacgctgatc
    14041 ccgctcgaca gttgggtcac tgaagggatc gactgggttg ttacccattt ccgtcccgtc
    14101 ttccagggcg tgcgcgttcc ggttgattat atcctcaacg gtttccagca attgctgctg
    14161 ggtatgccag cgccagtggc gattatcgtt ttcgctctca tcgcctggca gatttccggg
    14221 gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca tcggcgcaat cggtgcctgg
    14281 tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc tgctgttctg tatcgtcatc
    14341 ggtttgccgt tggggatatg gctggcgaga agtccgcgag cggcgaaaat tattcgtcca
    14401 ctgcttgatg ccatgcagac cacgccagcg tttgtttatc tggtgccaat cgtcatgcta
    14461 tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca tctttgcgct gccgccgatt
    14521 atccgtctga ccattctggg gattaaccag gttccggcgg atctgattga agcctcgcgc
    14581 tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc agttaccgct ggcgatgccg
    14641 accattatgg cgggcgttaa ccagacgctg atgctggccc tttctatggt ggtcattgcc
    14701 tcgatgattg ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg tcgtctggat
    14761 atggggcttg ccaccgttgg cggcgtcggg attgtgatcc tcgccattat cctcgatcgc
    14821 ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca accgtcgctg gtacaccact
    14881 ggccctgttg gtctgctgac ccgcccattc attaagtaac tctgcacttg cccggtgacg
    14941 ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc gacatagcgt actttttgcg
    15001 acagcgtttg ccacgcttat ctctacacaa acttttgctg ccgatctgcc gggcaaaggc
    15061 attactgtta atccagttca gagcaccatc actgaagaaa ccttccagac gctgctggtc
    15121 agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac ccagcgaagt ggattacaac
    15181 gttggctaca cctcgcttgc ttccggcgat gcaaccttca ccgccgtgaa ctggacgcca
    15241 ctccatgaca acatgtacga agctgccggt ggcgataaga aattttatcg tgaaggggta
    15301 tttgttaacg gcgcagcaca gggttacctg atcgataaga aaaccgccga ccaatacaaa
    15361 atcaccaaca tcgcacaact gaaagatccg aagatcgcca aactgttcga taccaacggc
    15421 gacggtaaag cggatttaac cggttgtaac cctggctggg gctgcgaagg tgcgatcaac
    15481 caccagcttg ccgcgtatga actgaccaac accgtgacgc ataatcaggg gaactacgca
    15541 gcgatgatgg ccgacaccat cagtcgctac aaagagggca aaccggtgtt ttattacacc
    15601 tggacgccgt actgggtgag taatgagctg aagtcaggga aagatgtggt ctggttgcag
    15661 gtgccgttct ccgcactgcc gggcgataaa aatgccgata ccaaactgcc gaatggcgcg
    15721 aattacggct tcccggtcag caccatgcat atcgttgcca acaaagcctg gaccgagaaa
    15781 aatccggcgg cagccaaact gtttgccatt atgcagttgc cagtggccga tattaacgcc
    15841 cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg atattcaggg ccacgttgat
    15901 ggctggatca aagcccacca gcagcagttc gatggctggg tgaatgaggc gctggcagcg
    15961 cagaagtaat ttttattcgg gcggataagg cgttttcgcc tcatccgccg ctctgtacac
    16021 aatgcctgat gcgacgctga cgcgtcctat catgcctaca aaccacatcg cacatttcaa
    16081 caagctatct ttcatcccat attcatcaac atccgctatt attgatttcc agcttaatca
    16141 tcacctgatg aacaaaaata atgactaaac ctaatcatga gcttagcccg gcgctgatcg
    16201 tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa ctattacgcc cagccattgc
    16261 tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc ggcaggcttt attgttaccg
    16321 ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt tcccctcggt gatatgtttg
    16381 aacgccgccg cctgattgtc tcgatgacct tactggcagc gggcggtatg ttaatcactg
    16441 ccagcagtca gtcactggcg atgatgatcc tcggtacggc attaaccggt ttattctcag
    16501 tcgtggcaca aattctggtt ccgctggcag cgacgctggc ttcaccggat aaacgcggca
    16561 aagtggttgg caccattatg agcggtctgc tgttggggat attgctggca cgaaccgttg
    16621 ccgggttact ggcaaacctc ggcggctggc gcaccgtctt ttgggttgct tcggtgttaa
    16681 tggcactgat ggcgctggca ttatggcgtg gtctgccaca aatgaaatca gaaacccacc
    16741 tcaactaccc acagttgttg ggttccgttt tcagcatgtt catcagcgat aagatcctgc
    16801 gcacccgcgc gttgctgggc tgcatgacct ttgccaactt cagcattctc tggacctcaa
    16861 tggccttttt gcttgccgct ccacctttta actacagcga tggtgacatt ggtctgtttg
    16921 ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc gggcggtttt gccgacaagg
    16981 gcaaatcgca ccacaccaca actttcggtc tgcttctgct gttactttca tggctggcaa
    17041 tctggtttgg tcacacttcc gtactggcgt tgattatcgg cattctggtg ctggacctca
    17101 ccgtacaggg cgtgcatatc actaaccaga cggtaattta tcgaatacat cctgatgcgc
    17161 gtaatcgcct gaccgcaggt tacatgacca gctactttat tggcggtgcc accggttcgc
    17221 taatttcagc ctcagcctgg caacatggcg gttgggctgg cgtttgtctg gctggcgcga
    17281 cgattgccct ggttaactta ctggtctggt ggcgaggttt tcatcgtcag gaagccgcaa
    17341 attaagcaat cagcaaccgt tttcgtggct ttacacttat aagggtgtta agaagcccat
    17401 cagtctgata aggttaagat attcattcag tctatttata atattaacaa tcgttaagcg
    17461 tacactctat ggaaagccct actccacagc ctgctcctgg ttcggcgacc ttcatggaag
    17521 gatgcaaaga cagtttaccg attgttatta gttatattcc ggtggccttt gcgttcggtc
    17581 tgaatgcgac ccgtctggga ttctctcctc tcgaaagcgt ttttttctcc tgcatcattt
    17641 atgcaggcgc gagccagttc gtcattaccg cgatgctggc agccgggagt agtttgtggg
    17701 ttgctgcact gaccgtcatg gcaatggatg ttcgccatgt tttgtatggc ccgtcactgc
    17761 gtagccgtat tattcagcgt ctgcaaaaat cgaaaaccgc cctatgggcg tttggcctga
    17821 cggatgaggt ttttgccgcc gccaccgcaa aactggtacg caataatcgc cgctggagcg
    17881 agaactggat gatcggcatt gccttcagtt catggtcatc gtgggtattt ggtacggtaa
    17941 taggggcatt ctccggcagc ggcttgctgc aaggttatcc cgccgttgaa gctgcattag
    18001 gttttatgct tccggcactc tttatgagtt tcctgctcgc ctctttccag cgcaaacaat
    18061 ctctttgcgt taccgcagct ttagttggtg cccttgcagg cgtaacgcta ttttctattc
    18121 ccgtcgccat tctggcaggc attgtctgtg gctgcctcac tgcgttaatc caggcattct
    18181 ggcaaggagc gcccgatgag ctatgaggtt ctgctgcttg ggttactggt tggcgcggcg
    18241 aattattgct tccgctattt gccgctgcgc ctgcgtgtgg gtaatgcccg cccaaccaaa
    18301 cgtggcgcgg taggtatttt gctcgacacc attggcatcg cctcgatatg cgctctgctg
    18361 gttgtctcta ccgcaccaga agtgatgcac gatacacgcc gtttcgtgcc cacgctggtc
    18421 ggcttcgcgg tactgggtgc cagtttctat aaaacacgca gcattatcat cccaacactg
    18481 cttagtgcgc tggcctatgg gctcgcctgg aaagtgatgg cgattatata acccacaaga
    18541 atcatttttc taaaacaata catttacttt atttgtcact gtcgttacta tatcggctga
    18601 aattaatgag gtcataccca aatggatagt tcgtttacgc ccattgaaca aatgctaaaa
    18661 tttcgcgcca gccgccacga agattttcct tatcaggaga tccttctgac tcgtctttgc
    18721 atgcacatgc aaagcaagct gctggagaac cgcaataaaa tgctgaaggc tcaggggatt
    18781 aacgagacgt tgtttatggc gttgattacg ctggagtctc aggaaaacca cagtattcag
    18841 ccttctgaat taagttgtgc tcttggatca tcccgtacca acgcgacgcg tattgccgat
    18901 gaactggaaa aacgcggttg gatcgaacgt cgtgaaagcg ataacgatcg ccgctgcctg
    18961 catctgcaat taacggaaaa aggtcacgag tttttgcgcg aggttttacc accgcagcat
    19021 aactgcctgc atcaactctg gtccgcgctc agcacaacag aaaaagatca gctcgagcaa
    19081 atcacccgca aattgctctc ccgtctcgac cagatggaac aagacggtgt ggttctcgaa
    19141 gcgatgagct aacgcgtcat ctcgctcaaa aatccagatt tataaaagaa aaaatgaccg
    19201 gccagcatcg caacatgctg gcctttttgg caagcaggtc ggctcagccg atgagttaag
    19261 aagatcgtgg agaacaatat gagcgcaaat gcggagactc aaaccccgca gcaaccggta
    19321 aagaagagcg gcaaacgtaa gcgtctgctc ctccttctca ccttgctctt tataattatt
    19381 gccgtagcga tagggattta ttggtttttg gtactgcgtc atttcgaaga aaccgatgac
    19441 gcatacgtgg cagggaatca gattcaaatt atgtctcagg tgtctggcag cgtgacgaaa
    19501 gtctgggccg ataacaccga ttttgtaaaa gaaggcgatg ttctggtcac tctcgacccg
    19561 acagatgctc gccaggcgtt tgaaaaagcc aaaactgcac tggcttccag cgttcgccaa
    19621 acccaccagc tgatgattaa cagcaaacag ttgcaggcga atattgaggt gcaaaaaatc
    19681 gccctcgcga aagcacaaag cgactacaac cgccgtgtgc cgctgggcaa tgccaacctg
    19741 attggtcgcg aagagctgca acacgcccgc gacgccgtca ccagtgccca ggcgcaactg
    19801 gacgtcgcta ttcaacaata caatgccaat caggcgatga ttctggggac taaactggaa
    19861 gatcagcctg ccgtgcaaca ggctgccacc gaagtacgta acgcctagct ggcgctggag
    19921 cgtacccgta ttgtcagtcc gatgaccggt tatgtttccc gtcgtgccgt gcaaccgggt
    19981 gcgcaaatta gcccaacgac gccgctgatg gcggtcgttc cagccaccaa tatgtgggtg
    20041 gatgccaact ttaaagagac gcagattgcc aatatgcgca tcggtcagcc ggtcaccatc
    20101 accaccgata tttacggcga tgatgtgaaa tacaccggta aagtggttgg tctggatatg
    20161 ggcacaggta gcgcgttctc actgcttccg gcgcaaaatg cgaccggtaa ctggatcaaa
    20221 gtcgttcagc gtctacctgt gcgtatcgaa ctggaccaga aacagctgga gcaatacccg
    20281 ctgcgcattg gtttgtccac gctggtgagc gtcaatacca ctaaccgtga cggtcaggta
    20341 ctggcaaata aagtacgttc aacaccggtc gcggtaagta ccgcgcgtga aatcagcctg
    20401 gcacctgtca ataaactgat cgacgatatc gtaaaagcta acgctggcta atccagaggt
    20461 gcgtgtgatg caacagcaaa aaccgctgga aggcgcgcaa ctggtcatta tgacgattgc
    20521 gctgtcactg gcgacattca tgcaggtgct ggactccacc attgctaacg tggcgatccc
    20581 cactatcgcc gggaatctgg gctcatcgct cagccaggga acgtgggtaa tcacttcttt
    20641 cggggtggcg aatgccatct cgatcccgct taccggctgg ctggcaaagc gcgtcgggga
    20701 agtgaaactg ttcctttggt ccaccatcgc ctttgctatt gcgtcgtggg cgtgtggtgt
    20761 ctccagcagc ctgaatatgc tgatcttctt ccgcgtgatt caggggattg tcgccgggcc
    20821 gttgatcccg ctttcgcaaa gtctattgct gaataactac ccgccagcca aacgctcgat
    20881 cgcgctggcg ttgtggtcga tgacggtgat tgtcgcgcca atttgcggcc cgatcctcgg
    20941 cggttatatc agcgataatt accactgggg ctggatattc ttcatcaacg tgccgattgg
    21001 cgtggcggtg gtgttgatga cactgcaaac tctgcgcgga cgtgaaaccc gcaccgaacg
    21061 gcggcggatt gatgccgtgg ggctggcact gctggttatt ggtatcggca gcctgcagat
    21121 tatgctcgac cgcggtaaag agctggactg tttttcatca caggaaatta tcattcttac
    21181 cgtggtggcg gtggtggcta tctgcttcct gattgtctgg gagctgaccg acgataaccc
    21241 gatagtcgat ctgtcgttgt ttaagtcgcg caacttcacc atcggctgct tgtgtatcag
    21301 cctcgcgtat atgctctact tcggcgctat tgttctgctg ccgcagttgt tgcaggaggt
    21361 ctacggttac acggcgacct gggcaggttt ggcctctgcg ccggtaggga ttattccggt
    21421 gattctgtcg ccgattatcg gccgcctcgc gcataaactg gatatgcggc ggctggtaac
    21481 cttcagcttt attatgtatg ccgtctgctt ctactggcgt gcctatacct ttgaaccagg
    21541 tatggatttt ggcgcgtcgg cctggccgca gtttatccag gggtttgcgg tggcctgctt
    21601 ctttatgccg ctgaccacca ttacgctgtc tggtttgcca ccggaacgac tggcggcggc
    21661 atcgagcctc tctaacttta cgcgaacgct ggcggggtct atcggcacgt cgataaccac
    21721 gaccatgtgg accaaccgcg agtcgatgca ccatgcgcag ttgactgagt cggtaaaccc
    21781 gttcaacccg aatgcccagg cgatgtacag tcaactggaa gggcttggga tgacgcaaca
    21841 gcaggcatca ggctggattg cccagcagat caccaatcag gggctgatta tttccgccaa
    21901 tgagatcttc tggatgtcag ccgggatatt cctcgtactg ctggggctgg tgtggtttgc
    21961 taaaccgcca tttggcgcag gtggcggcgg aggcggtgcg cactaagtac aactaagcca
    22021 gttcatttga actggctttt ttcaattaat tgtgaagata gtttactgac tagatgtgca
    22081 gttcctgcaa cttctctttc ggcagtgcca gttcttcgtt gctgttgatg cgtacgtcac
    22141 gttccagaat gctacgcgca atatcctgcg cttcctgcaa cgagtgcatc tggtaagtgc
    22201 cacactggta gacgttcagt tccgggatct gattctgatc ctgcactttc agcacgtctt
    22261 ccattgccgc tttccaggca tcagcaacac gctgctcatc tggcgtacca atcagactca
    22321 tataaaaacc ggtgcggcag cccattggcg agatatcgat aatctctaca ccattaccgt
    22381 taagatggtt acgcataaaa ccagcaaaca ggtgctccag ggtatggatc cctctttctg
    22441 gcatcacttc tttgttcggc acgcagaagc gcagatcgaa cacggtgatt gcgtcgccat
    22501 gcggggtgtt cattgttttc gccacccgaa ctgcaggcgc ttccatccgg gtatgatcga
    22561 ctgtgaagct atctaacaac ggcatttagc cacctccggt aattttttta aaaattttct
    22621 gaactctttc ttcccaggcg agtctgagta tatgaaagac gcgcatttgt tatcatcatc
    22681 cctgaattca gagatgaaat tttggccact cacgagtggc ctttttcttt tctgtcaggc
    22741 gtgtttttcc agccacaccg caaacggttc ggtatcagcg gtttccattt cctgctgacg
    22801 gcgttcagat gcctcgcgct cggctacaaa atcctcttcc cgcagaattt ccagcggctc
    22861 ttcacgcagc agattacggt aggcttctgc aaacgctttg cctgttccgc caataccagt
    22921 atcaatcata gaccttaaga tacgggcaga gaaagtcaga tcgggattat cgaagcaggc
    22981 aaccagttca tcacacactt tctgatacgc ttcgccgccg ttaatgctat ccagcgtttg
    23041 cgcgacgcgt ttcagatcgc ggaacagatc tttacccacc tgcagtaacg ggaactgcgc
    23101 ggtttcgcag ccgataccca gcgtcaggcc cggtttgcga ccttcaagga tcacccggtt
    23161 ccagttaacg cgcgtacagg caagttcgct actgctcatt tccggtgcat cagccagcgc
    23221 acaccagacc ataaacaggt cgaggaatcg cacctgctgt tcatctacac caatcggcga
    23281 gaacgggttg atgtccagcg aacgcacttc aatatattca atgccgccac gtaacagcgc
    23341 atcagaaggt gactcgccgc tgcgggtaac gcgttttgga cgaatcggcg catacagttc
    23401 gttttcaatc tgcagcacgt tgctattgat ttgcagcctc ttaccgtctt tctcaatacc
    23461 aatcttcgcg tactcttccg atggcgtttt gattgcctgt ttaaggcccg ctacgtactc
    23521 gtaaagatcg ttgaaggtaa taccaagatt gctttgcgat ttattggtat agccgagatc
    23581 gctcaaacga agagaggtcg catacggcag gtaatacata ccgcactcgg ttttctcaaa
    23641 cggcagcgac gttggttttc cttgcaggaa agaagaacaa atcgccggag atgcaccaaa
    23701 cagataagga atgacccaac cgaaacgata gtaattgcgg ataacgcgga aatagcccgc
    23761 agaaattttc tctttggcat cagcgcccga gatatcaccg cacttcgctt gccagaatgc
    23821 cattggcaaa gagaaattgt agtgcacgcc ggaaatggtt tgcatcagcg cgccgtagcg
    23881 atttttcagc ccttcacgat acagcgtttt aaagcgtccg gtgttagaag tgccgtactg
    23941 tgccagttcg atgtcctgac cttctgcgat gtagcatggc atacttaacg gccacatccg
    24001 ctcatcgccc atattgcgcg ccgtataacg atgcagatcg cgcataaagg tcagcatatg
    24061 ttcaatatca ccatccactg gtgtaatgaa ttccagcaat gcttccgcaa aatcggtagt
    24121 aatccatttg tgcgtcagtg cggaacctaa tgcttcagga tgacctgttg ttgccagtgt
    24181 gccatcagca ttaacacgca aagtttcgcg ctccagccca cgctgtatcc cctttaacgc
    24241 ctgaggatgt ttttccagcc aggccagcgc ctgtgatacg tccgggatca aattgacctc
    24301 ccgcctgtca aaatcgtttt aattagcata actgtaatgg tgaccatatg tgcaggccta
    24361 caattagtgc caccacatca tgccctgaac ggtcgctgct gcaactgcaa catagcgtaa
    24421 cgctttacca aggcacaaaa aaaagattac tggcccccag gagatgcgca tccatcccgc
    24481 taacagacac agtaaatcgc caaccacggg catccagctt aataatagcg tgactgcacc
    24541 atagcgtttc agccagccgg tagctttctc ttgccagcgc gatgttttac gcaatggaaa
    24601 gaaacgccca aggataacgt tagttaaccc tccaaggcta ttacccattg ttgctgttaa
    24661 aactaaaacc cagggatgac tgatcccgga aagcaacatt gccaccagca cgacttcgga
    24721 gttgccgggt aatagtgtag cgctgagaaa actactggca aacaacgaga aaagcgataa
    24781 cgcttcactc acagcaagcg aacatccaca gcgtccatgc ctgctgcacg ggccgcctga
    24841 ataccgaaat cggcatcttc aaagaccaca cactgcgtcg gttgcacgcc catacgctgc
    24901 gcgcacaaca aaaatgtgtc tggcgcgggt ttatggtgtt tgacgtgatc agcagcgacg
    24961 acggcgtcaa aataacggcg taatcccagg tgcgccagca atgcctcagc gatggcgctt
    25021 tcactccccg ttcctacagc cattgggcga cgaccatgcc aacttttcac cacttcaaca
    25081 agaggaagcg gctcgacact atccagcagc atacttctta ctgcttctgt tttttcacgc
    25141 gctaacgcat gcgggtcgag atcggcctga ttcagctcaa taattgcctg agcaatacgc
    25201 caggtgggcg atccattaag cgcaatcatc gcctgaatat cgtactgaag accgtagtgc
    25261 cctaatactt cgcgccacgc tttacggtgc gtaggctccg tatccaggat tgtgccatcc
    25321 atatcaaaaa ttaaacctgc ataacgctcg tacatggtct tctcgcaagt cgaaaaatat
    25381 gacgctactt tagcgtaatt gcttgatttt gtcgctgatg gcgaaggaga agaaaattca
    25441 ggaaatggat aaagtagtta tttagaaatg agatattttt gaaggaaatt ttttggagaa
//
LOCUS       DADLSB010000045        24961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.045, whole genome shotgun sequence.
ACCESSION   DADLSB010000045 DADLSB010000000
VERSION     DADLSB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.045"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(572..1348)
                     /gene="sseB"
                     /locus_tag="JN331_001813"
     CDS             complement(572..1348)
                     /gene="sseB"
                     /locus_tag="JN331_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY7259976.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGAAAQGE
                     AVVEDSVLDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSGQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1490..2773)
                     /gene="pepB"
                     /locus_tag="JN331_001814"
     CDS             complement(1490..2773)
                     /gene="pepB"
                     /locus_tag="JN331_001814"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY7259977.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2832..3032)
                     /gene="iscX"
                     /locus_tag="JN331_001815"
     CDS             complement(2832..3032)
                     /gene="iscX"
                     /locus_tag="JN331_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY7259978.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3044..3379)
                     /gene="fdx"
                     /locus_tag="JN331_001816"
     CDS             complement(3044..3379)
                     /gene="fdx"
                     /locus_tag="JN331_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY7259979.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3381..5231)
                     /gene="hscA"
                     /locus_tag="JN331_001817"
     CDS             complement(3381..5231)
                     /gene="hscA"
                     /locus_tag="JN331_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY7259980.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKTLAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5248..5763)
                     /gene="hscB"
                     /locus_tag="JN331_001818"
     CDS             complement(5248..5763)
                     /gene="hscB"
                     /locus_tag="JN331_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY7259981.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5859..6182)
                     /gene="iscA"
                     /locus_tag="JN331_001819"
     CDS             complement(5859..6182)
                     /gene="iscA"
                     /locus_tag="JN331_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY7259982.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6199..6585)
                     /gene="iscU"
                     /locus_tag="JN331_001820"
     CDS             complement(6199..6585)
                     /gene="iscU"
                     /locus_tag="JN331_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY7259983.1"
                     /translation="MAYSEKVIDHCENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6613..7827)
                     /gene="iscS"
                     /locus_tag="JN331_001821"
     CDS             complement(6613..7827)
                     /gene="iscS"
                     /locus_tag="JN331_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY7259984.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7939..8427)
                     /gene="iscR"
                     /locus_tag="JN331_001822"
     CDS             complement(7939..8427)
                     /gene="iscR"
                     /locus_tag="JN331_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY7259985.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8697..9437)
                     /gene="trmJ"
                     /locus_tag="JN331_001823"
     CDS             complement(8697..9437)
                     /gene="trmJ"
                     /locus_tag="JN331_001823"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY7259986.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9556..10359
                     /gene="suhB"
                     /locus_tag="JN331_001824"
     CDS             9556..10359
                     /gene="suhB"
                     /locus_tag="JN331_001824"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY7259987.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            10504..11358
                     /locus_tag="JN331_001825"
     CDS             10504..11358
                     /locus_tag="JN331_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7259988.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     APWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11549..12829
                     /gene="csiE"
                     /locus_tag="JN331_001826"
     CDS             11549..12829
                     /gene="csiE"
                     /locus_tag="JN331_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY7259989.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12821..13960)
                     /locus_tag="JN331_001827"
     CDS             complement(12821..13960)
                     /locus_tag="JN331_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY7259990.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGCEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYPYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14120..15010)
                     /gene="hcaR"
                     /locus_tag="JN331_001828"
     CDS             complement(14120..15010)
                     /gene="hcaR"
                     /locus_tag="JN331_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY7259991.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSTEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            15146..16507
                     /locus_tag="JN331_001829"
     CDS             15146..16507
                     /locus_tag="JN331_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY7259992.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWVLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16504..17022
                     /locus_tag="JN331_001830"
     CDS             16504..17022
                     /locus_tag="JN331_001830"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY7259993.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17022..17342
                     /locus_tag="JN331_001831"
     CDS             17022..17342
                     /locus_tag="JN331_001831"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY7259994.1"
                     /translation="MNRIYACPVEDVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17339..18151
                     /gene="hcaB"
                     /locus_tag="JN331_001832"
     CDS             17339..18151
                     /gene="hcaB"
                     /locus_tag="JN331_001832"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HAY7259995.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTCSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALEQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18161..19363
                     /locus_tag="JN331_001833"
     CDS             18161..19363
                     /locus_tag="JN331_001833"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAY7259996.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIAIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            19460..19882
                     /locus_tag="JN331_001834"
     CDS             19460..19882
                     /locus_tag="JN331_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7259997.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19930..20802)
                     /locus_tag="JN331_001835"
     CDS             complement(19930..20802)
                     /locus_tag="JN331_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY7259998.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGNKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20814..21896)
                     /locus_tag="JN331_001836"
     CDS             complement(20814..21896)
                     /locus_tag="JN331_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY7259999.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAKGRGAKRIIGVDMLPER
                     LAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTADWGR
                     VVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAITHRF
                     SLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21929..22927)
                     /locus_tag="JN331_001837"
     CDS             complement(21929..22927)
                     /locus_tag="JN331_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7260000.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVLID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIK
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22952..24463)
                     /locus_tag="JN331_001838"
     CDS             complement(22952..24463)
                     /locus_tag="JN331_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7260001.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     KGASKSTLIRKLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24486..>24961)
                     /locus_tag="JN331_001839"
     CDS             complement(24486..>24961)
                     /locus_tag="JN331_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7260002.1"
                     /translation="AFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKL
                     IISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAV
                     VDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
BASE COUNT         5881 a         6980 c         6229 g         5871 t
ORIGIN      
        1 tcagacaaat ataaacaaat cctgctcaaa attaaaaatt ctaaccggta aaagatatta
       61 cttaaacatg taaatttact ttcctttaaa aaacaaaaaa ccgccaaaat caggcggtta
      121 tttgttgctg gtccggttcg cggcctttcc agcaggttgt attaccgtag taatgcaagc
      181 gcgtctcagc ggagacaata ctcgccagta actctctttt tgtcaagcaa aagagagtta
      241 ttattgttct gttagtgtat tatccactgc ggccctttcc gccgtctcgc aagcgggcgc
      301 tggcttaagg aaaggatgtt ccgcgaccgt tagagcaagc gcgtctcagc gcttgctatc
      361 gcggcaatat cgccagtggt gctgtcgtga tgcggtcttc acatggaccg cacaatgaag
      421 atacggtgct tctgtatcgt acttattgtt tctggtgcgc tgttttccca agtaaataat
      481 aaccggagtc tctccggcga caatttactg gtggttaaca accttcagag cagcaagtaa
      541 gcccgaatgc cgccctttag ggcggcatat tttagattat ccggttttgt ttaaagtcac
      601 gcaaaaaacc accccagcga cgttcataga atggcgcaat atgttcggta ataaagtggc
      661 taattccttt ttcccctttt ttcacctgac aaatatcgat tggttcatcg ccaggtaatg
      721 tatcggtcgc tacacttccc gtcgcctgaa taatttcttc gatatcacca tcggcttcaa
      781 tgccaataag taaattaggc tgtgcctctt cgttctcttt aattgaacaa ataaaagcac
      841 gcttcaccgg cttaatggtt ttaaataagg tggtgagtga atcaatcatt tgtgctggcg
      901 gctctgcaac ttccgataat atcagcgatt caccgccttc caggatttcc tggccgctca
      961 gcggatttcc ctcttcacca atcaacaaac tgatttcacg cggcataaat tctttaccgg
     1021 tcggcagttt ggcattaagg aagagcgttt cgccaagggt catctcaaac aatgtgcgaa
     1081 cgggcattac gacaaatgcc tgttcgtctt caaccgcctg ttgaagcgct tctaacgagg
     1141 tgaaaaaagg aatgacgctg gtgccgtctt ctttttccca gtgctgtaaa tcaagcacac
     1201 tatcttcaac cacagcctcg ccctgcgccg ccgcaccagg cacccagacg gtggattcca
     1261 gtagagtacg gaaaaaggcc gggcggtgcg ccggttcggt tgctgctttt tccagcaggt
     1321 cttcaagttc gttttttgtt tcggacataa gaaccacaat tcattcaacg ttcggggcgc
     1381 aaatgcctga tgcgctacgc ttatcaggcc tacaagggat tcgcaatttg ttgaatttgc
     1441 agaatttgta ggccggataa ggcgtttacg ccgcatccgg catattagtt tacgccgtta
     1501 acagattagc tatcgtgcgc acaccaagtc ccgtagcgcc cgcagaccac tgttcaaccg
     1561 gcgctttacg gtaagtcgcc gagcagtcga tatgcagcca gccttgctga tagttctcaa
     1621 caaagtgcga caggaagccc gccgccgtgc tcgcgcctgc cggatacgcc gcgcttccgg
     1681 tattgttcag ttcggcaaag ttagacggca gctggctgcg gtggaattcc gccagcggca
     1741 gacgccagaa tggttcgttc tcttgtgagg cactcgccag taaacgaccg gcaagcgcat
     1801 cgtcaaaact gaacagcgcg tgataatcat tacccagcgc agttttcgcc gccccggtga
     1861 gggtcgccgc atcaatgatc atttccggtt tctgcgcact ggcatcaatc agaccatcgg
     1921 caagcaccag acgcccttcg gcatcagtgt tcatcacttc aactttttta ccgttgcgat
     1981 aggtgatgat atcgcccagc ttgaacgcat tgccgctaat caggttatcc gcacagcaga
     2041 ggaacagctt cacgcgcttg ttcagtccgc gcgtaatggc aaatgccagc gccccggtaa
     2101 ccgttgccgc gccgcccatg tccgacttca tcgagtccat aaacgcagtc tgtttgatgc
     2161 tgtagccgcc ggagtcaaaa gtgatacctt tacctaccag gcacgcgtac accggtgctt
     2221 ctttatcgcc agttgggttg taatccagcg ccagcaatac cggagaacgt tctgaaccgc
     2281 gcccgacagt gtgcagcccc atataacctt gctcacgcag atcttcgcct ttggtgatcc
     2341 gataagtcac acgatcgccc gcgacgttgc tgatcagatc aacagcacgc tgtgccagtt
     2401 gcgatggtcc caattcttct gccggtgcgt tgatggtgtc acgcacccag tcgatgatca
     2461 tcaggcggtt atccagttcc tggcgctggg catcgtccag atccggccac tccactttac
     2521 gcgtgccttt cgggcctttg taaccttgcc agaatgccca gcagcgatcc gcatcccagc
     2581 cttcaccgct taactgaaca tgcttgatgc ccagaccgtc aatcttgcgg gccgcacgct
     2641 ggatcagccc cagatcgtct gccccgttca aatgcagggt aatgccgtca ttattaatgc
     2701 tgtaagttgc tttttctccc cagcgcgcgt cggcaggttg ggtagagagg gtaatcttca
     2761 tcgcttctgt cattttagtt atccttattc gtttaacagc aaaacgggct acccccaggc
     2821 agcccgttca ttcactcggc ttcgtccagc cagactaaca aaatcgcttc gaggattttc
     2881 tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata
     2941 tcggtgaatc gaaccgtttt cggatcaaga tcgggatacg catcgtacag tgcttcgcca
     3001 atttcgcggc tatcggtcca cttaagtccc atactaacct ctgttaatgc tcacgcgcat
     3061 ggttgatagt gtaacgcggg atttcgacca ctaaatcttc gtcggtgacg cgcgcctggc
     3121 aacttaaacg gctttccggc tccagtcccc aggctttgtc cagcatgtcg tcttcctgct
     3181 ctgagctttc cggcagtgag tcaaaacctt cacgaacgat gcagtggcag gtggtgcaag
     3241 cacaggattt ttcacaggcg tgttcaatct cgataccgtt acgcagcgct gcgtcgagaa
     3301 tggtttcacc gctattagct tccagaacag cgccatcagg gcagaggtcc tgatgaggca
     3361 aaataacaat ctttggcata ttaaacctcg tccacggaat ggcctttcag cgcacgacga
     3421 accgactggt ccatgcggcg agcggcgaaa tcctgggttt gtttgtctac gtttttaatc
     3481 gcttgttcga tggcgtcaac atcatcgccc tgcgccactt cactcaggtg agcggcagca
     3541 ttgtcaatga cctgacgctc tgcggcgctt aacagcgcgg catcagcagc cagcgcgccg
     3601 tgcagacttt ccagcacacg cgccgcttct actttttgtt ctgccagcat acgggctttt
     3661 acgtcctgct cggcatagct cattgagtct ttgatcatcg aagcgatttc gctatcggtc
     3721 agaccgtaag acggtttgac ctgaatagac gcctcaacgc cggtggattt ctccatcgcc
     3781 gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg cgcaccgcca
     3841 gccggtagcg ccggaatacc acgcagcgca aaacgcgcca gtgagcggca gtcctgcacc
     3901 agttcgcgct caccctgcat tacatggata gacatcgccg tctgaccatc tttaaaggtg
     3961 gtgaaatcct gagcgcgggc caccggaata gtggtattac gcggaatcac tttctccacc
     4021 aggccgccca tcgtttcgag gcccagcgac agtgggatca catcaagcag cagcatttcg
     4081 ctgtctggct tgttacccac cagaatatcc gcctgaatcg ccgcgccaat agcgacgact
     4141 ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa attcgcctac ccgttcacgc
     4201 accagcggca cgcgagtaga accgcccacc atcaccactt ccagcacttc gtcagcttct
     4261 acacccgcgt ctttcagcgc gcgacgacaa gccagtaagg ttcgttttac cagtggcgcg
     4321 atcagttcat tgaattgttc acggctgatt tcgccctgcc agcccgcaac gttaacggtc
     4381 acggagtccg catcgctcag cgcgattttg gctgcaatgg cggcatccag cagttcacgc
     4441 tgaacgcggt tatcgctacg atcaggaatg tccgcctgct cgcgaatgta atccgccagc
     4501 agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc cggttgccag cacttcaaac
     4561 acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc cgccaccgag gtcataaaca
     4621 gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg
     4681 ttaagtaagc gcagaacatg caaccccgcc agacgcgccg cgtctttggt gccctgacgc
     4741 tgggcatcgt caaagtacgc cggaacggtg ataactacac catccagctc gcctgccagg
     4801 gcttcagttg cccgcgccgc cagtgttttg aggatgtccg cagaaacgcg caccgggttc
     4861 agtagccccg ccgccgtttc aatcatcggc aggccgtttt cgctggcctg gaattgataa
     4921 ggcagatgcg gatagcgttg ctggatatca gccagcgagc gccccatcag gcgtttaaca
     4981 gaactaatgg tgttggcggt gtcaagcgct gcgttagtac gcgcgtcata acccaccgaa
     5041 tgcccttgct gttgatagtg aacaacagat ggcagcaggt gacggccttc atggtcggct
     5101 aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg agttggttgt gcccaggtca
     5161 ataccagccg ccagacgacg ctgatgcggc gcagcactca aaccaggttc actaatttgt
     5221 aataaggcca tgtttagctt ccagaaatta aaaatcgagc agtttttctt cgagttgttc
     5281 ggcactgctc cgcagtttat cgagaaaacg cagcttacgc acggtatccg ccgccgcgtc
     5341 ccacgcctcg ttgtctaact gttcaaccat caactgatgg cgggtatcaa acatcttttt
     5401 cacacgtttg ataaagcttt ccagccgcgc ttcatctttc gcctgttcga tctcgtccag
     5461 ctcttcgcgc agctccaact gttccatcag gaacgcggtg tcgcgcacag tatgctgctc
     5521 gctggcgaga tcaaaaccgt gtaaagaaag caaatattcc gcgcgcatta acggatgacg
     5581 cagcgtttgc caggcctggt taatggttgc agattgctgt acggcggcga gttgttccgc
     5641 ctggcttccg ctggcgaatt tatcaggatg atactgacgt tgtagatcct gaaaacgcag
     5701 gctcagtgcc tgggtatcga gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc
     5761 cataacattc tcagggctgc gatccgccgc gataacggcg aatcaggtaa aacaaacccc
     5821 acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg
     5881 caaccacact catctttaac gttcgggttg gtgaatttaa acccttcatt caggccttct
     5941 tttacgaagt ccagctgcgt accgtccaga aattgcaggc ttttgccatc gaccacgact
     6001 ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac aaattccagt
     6061 acataagcca tacctgaaca tccggaggtt ctcacgccca gacgcaggcc aaaccctttg
     6121 ccgcggttag ccagaaaggt atttactcgc gctgctgcac tgtcgctcag tgtaatcgac
     6181 ataaccaaac ctcaactctt attttgcttc acgtttgctt ttatagtccg caatggcggc
     6241 tttgatcgcg tcttctgcca gaatagaaca gtgaattttc accggcggca gttcaagttc
     6301 ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg agagacttcc ctttcaccca
     6361 ttcggtgacc agggagctgg aagcgatagc ggaaccgcag ccgtaagttt taaaacgcgc
     6421 gtcttcaatg ataccttcat cgttgacttt aatctgcaac ttcattacgt cgccacaggc
     6481 cggtgcaccc accatgccgc tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt
     6541 acgcggattc tcgcaatggt cgataacttt ttcgctgtaa gccattataa attctcctga
     6601 ttccgatacc gattaatgat gagcccattc gatgctgttc agatccacgc cctgcttgta
     6661 catttcccac agcggagaaa ggtcacgcag acgaccgatg gatttacgaa ctaactcgat
     6721 ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa gagaaacgga tagagctatg
     6781 tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga ggcttgctga
     6841 cgtacaggcg gaacctgaag aaactgcgag gtctttcagc gccataatca gcgactcacc
     6901 ttcaacgtag ttgaagctga cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt
     6961 caggtaaact tcttcgatat ctttgatgcc gttccacaga cggttacgca ggccgcgcag
     7021 acgttccatc tcggtcgcca tctcttcttt tgcgatgcga taggcttcgc ccattccgac
     7081 gatctggtga acaggcagag tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat
     7141 ttgcgcttcg atgcgtacac gcggtttacg acgcacatac agcgcaccga tacctttcgg
     7201 gccatagatt ttgtgaccgg agaaagacat caggtcaact ttcaactggc tcaggtcgat
     7261 tggcagtttg cccacgctct gggttgcatc aacgtgatag ataatgccac gagcacggca
     7321 catttcgccg atagccgcga tatcctgcac cacgccgatt tcgttattta cgtgcatgat
     7381 ggaaacaagg atggtgtcgt cacgcatcgc cgcttcgagt tctttcaggt cgataatacc
     7441 gttacgctgc ggtgccaggt aggtgacttc gaaaccttcg cgctccagct gacggcaagt
     7501 atccagtacc gctttgtgtt cggttttgct ggtgatgatg tgcttgcctt ttttctgata
     7561 aaagttggct gcacctttga tcgccaggtt gtcagattcg gttgcaccag aggtaaagac
     7621 gatttcacgc ggatcagcgc cgaccagatc ggcaatctga ttacgggcga tatctaccgc
     7681 ttcttcagcc tgccagccga aacggtgaga acgagaggcc gggttaccaa aggttccgtc
     7741 catcgtcatg aactgcatca ttttctcggc aacacgcggg tccaccggcg tggttgcgga
     7801 gtagtcgaga taaatcggta atttcattgc tctataaact ccgtacatca ctcaatgcaa
     7861 ggaatcaggc taccggctgg atgtacgacc gtgtttacgg agtatttagc actccggcct
     7921 gattctgaat tctttttatt aagcgcgtaa cttaacgtcg atcgcgtctt gtgtgcgggt
     7981 gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct ggttattaac
     8041 cagttcgcct aaagtaatgt tgttgagaaa accggtgaga cggtcgctca aatcacgcca
     8101 cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg cctttaccct gacaacgggt
     8161 ggcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc tgctggcatc
     8221 tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac catttttacg
     8281 cagacgggaa aacagttgtt ccagataaga aagggaaatt ccctgacgtt cggaaatatc
     8341 agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg cggtcacggc
     8401 atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc ccctgcccgg
     8461 ggttttttat tgtaaaagtg ggggtattgc atagcagggt caagtctgac attcccgagt
     8521 aaattggtca actatttact tgactgattt agtcgggtat ttaaccttca gtgccatttt
     8581 tttatcgtgg cgtttgtacc tgttgtcgga tgcggtgtga acaccttatc cgacctacga
     8641 ttctgtacat tgtaggcctg ataagacgcg ttaagcgtcg catcaggcaa ccactgttat
     8701 tcggccttgt tacctttatt ctgctgctca atagaagcca gaatcccgcg caggatattc
     8761 aattcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt catcacctgc
     8821 cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag atgaccataa
     8881 aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac ctgctcgcca
     8941 ttttcctgag ttgccagcca ggccatacgc acttcatagg caataacctg aaccgccatt
     9001 gccaggttca gcgaactgta ttccgggttt gccgcaatcg cgacatgata atggcatttc
     9061 tgcaactctt cattggtcaa gccgacgcgc tcacgaccaa acaccagcgc caccggggta
     9121 tttgccgctt cagcgacgct tttcagaccg cattcgcgcg ggtcgagcat cggccacggc
     9181 agcgtgcggg agcgtgcgct ggtgcccacc accaggctac aaccggctaa cgcttcgtcg
     9241 agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccctgctgc cagggcaatc
     9301 gcctgggagt cgggtttcac cagtggatta accagccaca gattggttaa tcccattgtt
     9361 ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac cagcacaatt
     9421 cgaatatttt gcagcattgt ctttcttcat ctaaagatta ttcacgcatc ttatcataaa
     9481 acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt ctttaacatc
     9541 cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg
     9601 ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc
     9661 agtaacgatt tcgtgaccaa cgtagataaa gctgccgaag cggtgattat cgacacgatt
     9721 cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact tgaaggtact
     9781 gatcaggatg ttcaatgggt tatcgatcca ctggatggta ctaccaactt tatcaaacgt
     9841 ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag gccgcaccga agttgctgtg
     9901 gtatacgatc ctatgcgtaa cgaactgttc accgccactc gcggtcaggg cgcacagctg
     9961 aacggctacc gtctgcgcgg tagcaccgct cgcgatctcg acggtaccat tctggcgacc
    10021 ggcttcccgt tcaaagcaaa acagtacgcc actacctaca tcaacatcgt cggcaagctg
    10081 ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtc
    10141 gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc gtccgtggga tttcgcggca
    10201 ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg acttcaccgg tggtcataac
    10261 tatatgctga ccggtaacat cgttgctggt aacccgcgtg ttgtgaaagc catactggcg
    10321 aacatgcgtg acgagttaag cgacgctctg aagcgttaat gactcaggcg ggtgatatca
    10381 ctcacccgcc ctcgcctttc aggcgctatt ccgaaatact tcctcaccgc tttactttct
    10441 ttcgtcactc tcccaccatc tttccccgat taatggatga taaagaagtc gcaaggaaag
    10501 gttatggcac tgccagtgaa caaacgcgtt cccaaaattc tgtttattct ctttgttgtt
    10561 gccttctgcg tttatttagt gccgcgcgtt gccatcaact tcttctatta tcctgacgat
    10621 aaaatttacg gtcccgcccc ctggtcggcg gaatccgtcg aatttacggc taaggacggt
    10681 actcgtctgc aaggctggtt tattccttct tcgacgggcc ctgctgacaa cgccatcgca
    10741 accatcattc atgctcacgg caatgccgga aatatgtccg cccactggcc gctggtcagt
    10801 tggttacctg agcgtaattt caacgttttt atgtttgatt atcgcgggtt tggtaaatca
    10861 aaaggcacgc cgtcccaggc cggattgctg gacgatacgc aaagcgccat caatgtggtg
    10921 cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag tattggcggg
    10981 gcgaatattc ttgctgttat tggtcagggt gatcgtgaag gcatacgtgc ggtgatcctc
    11041 gactccacat ttgcctctta tgcaaccatc gccaaccaga tgatccccgg cagtggctac
    11101 ttacttgatg agagttacag cggcgaaaat tacatcgcca gcgtcagccc gatcccgctt
    11161 ttactcattc acggtaaagc cgatcacgtt atcccatggc agcacagcga aaagttgtat
    11221 agcctggcaa aagagccaaa acggttgatc ctaatcccgg atggcgaaca cattgatgct
    11281 ttttccgatc gtcacggcaa tgtttatcgc gaacagatgg tggactttat ccttagcgcg
    11341 ttgaatccgc agaactaacc catgatcgct agcacgataa tcattcacaa aaccacctta
    11401 agacatgcta atccactggt cagaacagtt taaaatgaga aaaattctgt gacgcttgcc
    11461 aacatttctg atgattagca ttcccttcgc catttccctg agcaaacttt agctattctt
    11521 atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac catctgtcct
    11581 ttcggctccc cagcgccgct gccagatctt gctgacgctc tttcagccgg ggttgaccgc
    11641 caccatggca accttcagcg agcttaatgg tgtggatgat gatattgcca gtcttgatat
    11701 cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc acactgacga ctggttatga
    11761 cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat ttcactggct
    11821 acgacgtggt ttccgtctgt gtccgtcatt tattaccagc caattcaccc ccgccctgaa
    11881 gagtgaactg aagcggcgcg gaattgcgcg taacttttac gacgatacca atctacaagc
    11941 gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaatcgcgcg atattcattt
    12001 cctgtgtctg tatctgcaat attgtttgct gcagcaccac gctggaatta cgcctcagtt
    12061 taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat
    12121 tggacgccac tggcagcgtc gggcgctcca gcatgtaccg cctgatgagc cactgtttat
    12181 ggcattactt ttttccatgt tgcgggttcc cgatccactg cgggatgcgc atcagcggga
    12241 cagacaattg cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt
    12301 tcgtttttat gatgaacagg ggttatgtga tcagctttat atccacctcg cccaggcgtt
    12361 aaatcgcagt ttgtttgcca tcggtattga taataccctg ccggaagagt tcgccagact
    12421 gtatccacgc ctggtacgca ccacccgcgc ggcgctggcc ggatttgaaa gtgaatacgg
    12481 cgtccatctt tctgatgagg aaagtggtct ggtcgcggtg attttcggtg cctggctaat
    12541 gcaggaaaac gatctgcatg aaaaacagat tattctactg accgggaatg atagcgagcg
    12601 agaagcgcag attgagcagc agttacgcga actaacgtta ctgccgctca acattaagca
    12661 tatgtcggta aaggcatttt tgcagacagg cgctccgcgt ggcgcggcac tgattattgc
    12721 gccttatacc atgccgttac cgctcttttc accaccgctg atctatacgg acctgacgct
    12781 gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag ggaacaactt
    12841 tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat tacccagaac acgccgtggc
    12901 ccagatatgg atacaggaaa ccggcgaaaa cggtcatgat agcgatactg ccgcccatcg
    12961 cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgca accctggcga gcagcaatat
    13021 aacgcatggc ggccaggtgg cagaccgtga aggtgccgca atgcagaatt tgcaccacta
    13081 tcaaccacgg caacgccgta gttgctccca taatgcccca gcgcactacg ccgcaaatcg
    13141 ccgagatcaa cagcatatcg cgagcactac aacggcggaa aagtttatta ctcagcgcaa
    13201 agataatgac ttccgccacc acgcccagcg accacaaata ccccaccgcc gaggccgagt
    13261 agccagctgc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgt gccccctgca
    13321 ataaacaaac gcaggccaga aagcgccagt tctggcgaac cagcgccaac cacgcagacc
    13381 aaccggtgct ctcctgctgg cggcttgccc cttgtggctg aatcgtcgga cggatgagaa
    13441 agccgagcag catggatgcc acgcccaacg tcaacagcac gaggatcacc cgataatcaa
    13501 acatagtgac cagtttgccc gtcagcgccg agccaatgac aaacgccacc gagccccaca
    13561 gtcgcacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc agtgcatcgg
    13621 tcaacggtac cagcggtgag aaaaagaggt taaagccaat catcaccagc atcagccacg
    13681 ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tggcagcagt gccagcacgc
    13741 gcaaggcgga aatcaggcgg gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga
    13801 gaaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc gttaaaccaa
    13861 tccctttaag ccagacgctc cagaaaggta aaaaaatgcc gtaactaaaa aagtatgtga
    13921 aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg
    13981 cgttagtctg gggttaatcg aatgttggtg caagttgaaa aataagaata ttcgagcctg
    14041 cttcccgatg tctgtaacgc cagttcacaa gcactaaaaa actgcgatcc gcgacgcatg
    14101 ttgcaaaaat aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc acaatggcga
    14161 tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt aaagcaatgg
    14221 aaggcacatt tccggcaatg ggacgaaaaa caacctgccc ggtattaaaa ttattcatat
    14281 aaccgggtat caaagtgacg cccagcccca tgcccaccag attcatggtc accagaatat
    14341 tcgttgccac ctggacgata tttggctggc tgttttcttg cgcaaaccac gctttaacga
    14401 tcggcgcaag cgaaccggaa tacgccggat cggtactgac gaaattcacg ccatccagtt
    14461 gcgcggcggt gatctctttt tcatgtgcta aagggtgatc aaccggtaac accaccacta
    14521 atggttcgtc aaaaagctcc agataatcaa tctccgtgct ataaacagga tggcgcatta
    14581 agccgacgtc gagttcacca cggcgaattt tttcctcctg ttgcgtggtg attaaactca
    14641 ccagctcaat caaggtgtct ggctgtctga gacgaaacat cggtaatact tttggcaata
    14701 aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc tgaacaattt
    14761 tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg tcctggagaa
    14821 aacattctcc cgccgccgtc aacgcgactt tgcgcttatc cctcaccagt aacggaacac
    14881 cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag gtatgcagtt
    14941 tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag cgtaaatgcc
    15001 gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag aaattaacta
    15061 tttcacatat tagcaaccaa ccagcaacat ccttatggca caaaaataga aggtcaatac
    15121 atcttatctt tcaggattaa aaaatatgac cacaccctca gatttgaaca tttaccaact
    15181 gattgacacc caaaacggtc gggtcactcc gcgtatttat accgacccgg acatttacca
    15241 actggagctt gagcgtattt tcggtcgttg ctggctattt ctcgcccacg aaagccagat
    15301 cccaaaaccc ggtgatttct ttaacaccta catgggagaa gatgcagttg tcgtggtgcg
    15361 ccagaaagac ggcagcatca aggcgtttct caaccaatgc cgccaccggg ccatgcgtgt
    15421 gagttatgca gattgtggca acactcgcgc ctttacctgc ccgtatcacg gctggtctta
    15481 tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc cacaagggtt
    15541 gtgtaaatcc cactgggtgc taaacgaagt tccttgtgtg gagagttata aaggactgat
    15601 ttttggcaac tgggatacca gcgcaccggg cctgcatgat tacctgggtg acattgcctg
    15661 gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg gcggcgtaca
    15721 aaagtgggtg atcaactgta actggaaatt cccggcagag cagttcgcca gtgaccagta
    15781 tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag atgatggcag
    15841 cgataagcgc ctcggtgatg gacaaaccgc ccgcccggtg tgggaaaccg ccaaagatgc
    15901 gctgcaattt ggtcaggacg gtcacggtag cggtttcttc tttactgaaa aaccggatgc
    15961 taatgtctgg gtcgatggcg cagtttcaag ctattaccgc gaaacctatg ccgaagcaga
    16021 acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata tattccccac
    16081 gctttcatgg ctaaacggca ctgccacact ccgcgtctgg catccgcgcg gccctgatca
    16141 agttgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag ttaaagccgc
    16201 ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc aggatgactc
    16261 ggagaactgg tgtgaaatcc agaaattgct taaaggccac cgcgcccgca acagcaaact
    16321 gtgtctggaa atggggcttg gtcaggaaaa gcgccgcgac gacggcattc ctggcattac
    16381 taactatatc ttttcagaaa ctgccgctcg tggaatgtac caacgctggg ccgatcttct
    16441 gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc aggaggtgat
    16501 gaaatgagtg cgcaagtttc actggagtta catcaccgca ttagccagtt tctctttcac
    16561 gaagccagtc tgctggatga ctggaaattt cgtgactggt tggcacagct cgacgaagag
    16621 attcgttaca ccatgcgcac cacggttaac gcgcaaacac gcgaccgtcg caaaggcgtc
    16681 cagccgccga cgacctggat tttcaatgac accaaagacc agctggagcg gcgaatcgcc
    16741 cgtctggaaa cgggcatggc ctgggcagaa gagccgccgt cacgcactcg tcacttaatc
    16801 agcaactgcc aggtaagcga aaccgacatc ccaaacgtat ttgccgtgag ggtgaattat
    16861 ctactttatc gggcacaaaa agagcgcgat gaaacattct atgttggaac gcgtttcgac
    16921 aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat cgtcctagat
    16981 caagcggtga tcacttccca taacctgagt gtactgttct gatgaatcga atttatgcgt
    17041 gtcccgttga ggatgtgccg gagggtgagg ctctccggat agatacctcg cccgtcatcg
    17101 ccttgttcaa cgtgggcggc gagttttatg ccattaacga tcgttgcagc catggtaatg
    17161 cgtcgatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg ctacacgccg
    17221 ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat ccgctcacca
    17281 cttatccagt acacgttgaa ggtggtgaca ttttcatcga cttaccggag gcgcagccat
    17341 gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat tagggctggc
    17401 gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac tgtcggcggc
    17461 aaaagtcgcc agtctgcgtc agcgatttgg cgaacatatt ctggcggtgg aaggtaacgt
    17521 gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgacttgtt ccggcaagct
    17581 ggattgtttt atcggcaatg caggcatctg ggatcacaat gcctcactgg ttaatactcc
    17641 cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaacgtac tcggttacct
    17701 gctgggcgca aaagcctgcg ctccggcgtt aatcgccagt gaaggcagca tgattttcac
    17761 actgtcaaat gccgcctggt atcctggcgg cggtggcccg ctgtacaccg ccagtaaaca
    17821 tgccgcaacc ggacttattc gccaactggc ttatgaactg gcaccgaaag tgcgggtgaa
    17881 tggcgtcggc ccgtgtggta tggccagcga cctgcgcggc ccacaggcgc tcgagcaaag
    17941 tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt gccgccattt taccgctgca
    18001 atttttcccg caaccggcgg attttacggg gccgtatgtg atgttggcat cgcggcgcaa
    18061 taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcga ttcgcggcat
    18121 tcgccacgta gcggctgggc tggatcttta aggaagcacg atgaaagaaa aaacgatcat
    18181 tattgtcggt ggcgggcaag cggcagcaat ggctgcggcc tcgctacgcc agcaagggtt
    18241 caccggtgag ctgcatctgt tttccgatga gcgacatctt ccttatgaac gacctccact
    18301 ctcgaaatcc atgttgctgg aagattcccc gcagttacag caggtgttac ccgctaactg
    18361 gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacgc tgggtcgcga
    18421 cacacgagag ttagtgttaa ccaacggcga aagctggcac tgggatcagc tttttatagc
    18481 aaccggcgcg gcagctcgac cgctgccgtt gcttgatgca ctgggagaac gctgctttac
    18541 cctacgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg aacggtcagt
    18601 cgtgattgtc ggtgccggaa ctattggtct ggaactggct gccagcgcca cgcagcgcag
    18661 atgtaaggtg acagtgattg aactggcggc aaccgtcatg ggccgtaatg caccaccgcc
    18721 cgtgcaacgc tatcttttac agcgccatca gcaggctggt gtgcgcattc tgctcaataa
    18781 tgccattgaa catgtggtcg atggtgaaaa agtagaactg acgctgcaaa gtggggaaac
    18841 gcttcaggct gatgtggtga tttacggtat tggtatcagc gccaacgagc aactggctcg
    18901 cgaggccaac cttgatactg ccaatggcat tgccattgat gaggcttgcc gcacctgcga
    18961 tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt cttgataatg gtgcactaca
    19021 ccgctgcgaa agctgggaaa acgccaataa ccaggcgcaa attgccgctg ccgcaatgtt
    19081 agggctaccg ctaccgctac tgccgccgcc gtggttctgg agcgatcagt acagtgataa
    19141 cttacagttt attggcgata tgcgtggcga tgactggctt tgtcgtggca acccggaaac
    19201 tcagaaggcg atttggttta atctgcaaaa cggcgtgctt atcggtgcgg tcacgctgaa
    19261 tcaggggcgt gagattcgcc caattcgcaa atggatccag agcggcaaaa cgtttgatgc
    19321 gaaactgctg atagatgaga acatcgcgct taaatcactg taaccaggat aattagcgaa
    19381 tatctcaatg cctggggcgt ggcgaggtgc aagagtgtgt attacgttta aatcacatta
    19441 tcttgcaaag ggattggtta tgaacacact acgttatttt gattttggag ctgcccgccc
    19501 cgttttgtta ttaattgccc gtatcgccgt ggtcttaatt ttcattattt ttggttttcc
    19561 caaaatgatg ggctttgacg gtacggtcca atatatggcc tcgttgggcg cgccaatgcc
    19621 gatgctggca gcgattattg cggtagttat ggaagtgccc gccgcgatat taatcgtgct
    19681 tggctttttc acccgtccgc tggcggtgct gtttattttc tacacgctgg gtacggcggt
    19741 gattggtcac cattactggg atatgaccgg cgatgcggtt gggccaaata tgattaattt
    19801 ctggaagaat gtcagtatcg ctggcgcgtt cttgctattg gcaattaccg ggccgggggc
    19861 aatttctctc gatcggcgtt aggaaagatg ccggatgcgg cgtgaacgcc ttatccggca
    19921 ttttataaat tacagccact ctacccgcaa cgacatctct gaggttgttg attcccctgg
    19981 tgctagcgca atgagatcac ccccttccgg gcgatgatga tcatccggcg catgactcat
    20041 cggttccaga cagaagaaat caaacgcata tcctttatca aacgcagggt cggaaacaaa
    20101 gatgaaataa cacggtgcag gtggcgtcgt ttccatgatg atggcataac cttcctgcgg
    20161 ctgctcgatg cgggcctgac cattccatcc ggcaaaacca ttgttcaccc actgacgcgg
    20221 caacggcgcg agctggttaa aatctagttc ctgcggtagc tgctcgcaaa actcacccgc
    20281 cagccactgc tcccgttcca gccagtaacc gctcgcctgc gcctgaatcc gcgtttgtgg
    20341 tgacaacgga aaataaggat gccagccggt accaaatggc agcgtctctg ccccttgatt
    20401 ggtgacagag agcgtcaccg tcagcgtatc cgccgttaaa tgaaacgcct gacttacccg
    20461 atagtgatag acaccgctgc gatgttcata caccagacac aaactatcat cgctgtgcga
    20521 gacacattgc cactcgccca gccagccatc gccgtgtaga tagtgcgcat cccactcaac
    20581 attcggttgc agttgatact cacgcccctg ccagacaaaa cggttgccgc tcacccggtt
    20641 tgcaaatggc accagcggaa aacatgaggc atcggttgcc acaccgcttt tgttaccagg
    20701 acgtaagagc ggcgtcgtat cgcgccagaa gccttcgatt acgccgccct ggtcagaaac
    20761 gtccagcttt agcgacccgt gggataaggt atagatggtc atccgctaac tccttaatcc
    20821 gggaagttaa tcacaacttt cccgcatttg ccgctcgcca tcagcacata ggcatcacct
    20881 gcctgttcta gcgagaagcg atgggtaatg gcgttacgcg gccacagctt ccagtcagtc
    20941 aaatcgtggg cgcatttttc catatggaac agactggtca cccaggagcc gataatccgc
    21001 cgttgatggt gcatcagatc agcgctgacc tcgaattcca cttttccggt ttcaccaatg
    21061 taaaccaccc gcccccagtc agcagtggat tgcagtgcca gcaagcgacc tgcggcatta
    21121 ccggaacaat cgagcgcaac atccgcgcca ccgtgggtaa gttcagcaat tatctgcggc
    21181 agaccttcgg tggttgctaa atagccgtga tccatcaccc ctaactgttt tgccatcgcc
    21241 agacgttccg gcagcatatc aacgccgatg atccgttttg caccgcgacc tttcgccatc
    21301 atgccgaccg gccccagacc gaccaccagc acgttatcgc tgccggaaac ttcgccgcgc
    21361 aaaatccctt cataggccgt gccaacgccg caactgataa acgcaccatc ttcgtagctc
    21421 agcgcatccg gcaggaggat cagatctttt tcttccgcca gcagatattc ggcatgaccg
    21481 ccgtcacgct gccagccgta agccgctttt cctttgccag tacaagagat aggaaagccg
    21541 cggcggcagt tcgggcaaaa accacagcca gaaatgtgat acaccagcac gcggtcgccc
    21601 tctttaaaat ggcggcagcc ttgccccatc gccacaatct gcccgcacgg ttcatgaccg
    21661 ttgataaatc cctggtataa cggtttatcg ggtgccgccg ccgtggcacg gtgttgatga
    21721 tagatatagt ggacatcgct tccgcaaatc ccggaggatt tcattttgat cagtacctgg
    21781 ttaatccccg gcgtcggcac cgcaacttcc cgcagatcga cggttgaatt tcctggtaaa
    21841 taagctgcca gcatcgtttt cataaatccc tcattaacaa tacgattaat tttcatccct
    21901 gccgcacccg cgccaggggc gttaaggttt agcgtttcgc tttactgctt cgctgggtca
    21961 gcaagatatt cgccagcacc gccaccacga tgatgacgcc gcgtaccacc tgctggaaaa
    22021 aggagttaat accgagcagc accagaccgt taccgattag cgtaatcacc agcacaccaa
    22081 gcaatgtacc gaacagggaa ccgcgaccgc cggaaagtgc cgtaccgccg accacgaccg
    22141 cggcgatgac gtcaaactcc agaccgtttg cggcacctgc gttaccggaa ccgaggcgtg
    22201 ccgccaacaa aatgccggtc accgccgcta ataatcccga aagggtaaag ataagaatgc
    22261 gcactcgacg aactttaata ccgcacaact gcgccgccgt ggcattaccg ccaacggcaa
    22321 aaaccgagcg cccgaaggcg gtttttcggc taatgaacac aaacagcgca aacaacacca
    22381 tcatgatcag cgcggatact ggcacaccga gaaattgtcc gcccagccag tccagcacct
    22441 cgttttcgtc aatcagcact ggcagtgcgt tggtcataaa cagccccatt ccgcgcaggg
    22501 cgctccacag ccccagtgtg gcaacgaaac ttggtacgtt aaacacgccg cgcagcaccc
    22561 cggcgagcgt tcccatcagc gcgcctaaca gcaacaccag cagacaagca atcgccaacg
    22621 gaacttcaaa ttgcagcaaa aatgccaggc acaccgagac aaaagccacc atcggcccaa
    22681 cgctgacatc aatttcaccg gagataataa tcagcgtcat cgcccaggcg gcaatcccaa
    22741 tggtggcggc atcgcgcagc acgttcatct ggttattcaa tgagataaag ccaggcgcgt
    22801 tcagggagaa gaccagataa agaatggcaa tcaccaccag caaaccgatc tcattaatat
    22861 ggcgactgac aaattgtttg agcgagacgc tcttgccctg cggcaatggt aatgacgaag
    22921 cagacatctt caggttcctc ggtaaaattc atcagtgcac agacagaatg gcggacatca
    22981 gctcatccac attgaccggc gagtgaaact cctgcgagaa cgtgccgtgc tgtaataaca
    23041 ggatgcggtc acacaccagc ggcaactcct ccacttcact ggagataaac accacgcttt
    23101 ttccttcggc agccagctca cggacaatac ggtaaatctg ctgtttggct tcgatatcga
    23161 cgccgcgcgt tggctcgtcg agcaacaaaa tctggctggc ggcatagacc caacgaccga
    23221 tcaccacttt ttgctgattg ccaccagaaa gcgtgccgat gggtgtttcg ctactggcag
    23281 ccttgaccgt catccgctgc atcacctctt cggtcaggcg gcggatggtg gaccattgca
    23341 gcacaccgtt ggtgctgatt ttttgccgat tggtcagcac tgtattttcg tcaacgccca
    23401 gccagggaat gatccccgct tctttgcggt tttctggcgt atagccaatg ccgcgtttca
    23461 gcatgtcgcc gtaatcgggg cgcatgattt tctcgccgtt gataacaatt tcgccctgtt
    23521 catacgtctc cagcccaaca atcgccttca gcaattcact gcgccctgcc cccagcaggc
    23581 cagcaatgcc aagcacttcg ccacgacgta gcgtaaagct gatatcctcc agcttgggct
    23641 tatggcgtaa cgcacggact tccagtacgg cctgatccac aatttcctga ggggcaaccg
    23701 gcgcaatatc aacgtgatca cgccctagca ttagcgacac aatatgatgc gtggaggtgt
    23761 tttcgagcat cacatcgccc gccacctgac catcgcgcat aacggtggca caggaggcaa
    23821 tgcggcggat ttcttccatc cggtggctga cataaatcac cgccacgccc agtgccgaca
    23881 tctttttcac cgcactgatc accagttcaa cttctgcact ggcaagcgag ctggtgggtt
    23941 catcaagaat gaccacgcgc ggctcgccct tcatcacccg tgcaatttcc accagttgct
    24001 tttgcgccgg gcttagcgtt gaaacaagtt gttcaggact aacgtcaacg cccagcgcct
    24061 gtaagcaacg ttgggcatcc tgcgccattt gcaggtaatc aatcatgccg ttgcggcgag
    24121 gccattgacc aaggcagagg ttttctgcca ccgtcagccc ttccaccaga cttaattcct
    24181 gataaaccgc acgaaccccc agttcagcgg cacggcgagt caacgtagct tcgtcacctt
    24241 ccagtcgcgt ctcgccaatc cagatatcac cgctatccgg gcgttcgctg ccggtaagct
    24301 ttcgaatgag agtcgatttg ctcgcgcctt ttttacctaa cagcgcacga acttcgcctt
    24361 tattgagcgt gaagttaacg ttatccagcg caacgacgcc ggggtaacgc ttatttcctg
    24421 ccaccacttt tgctaccggg actgcctctg ttgccgtgaa catagggacc tctgcgaatc
    24481 agcgattagg gcagaccatc aacgtgcgtt gccagccact gtttaccatc ttccgttttg
    24541 gtatagagat cgattggcac ctgaatcact ttttcaccgt cggcttgttt attgataacc
    24601 ttcaatgttt gcgcgaaaac agcattgccc attttcttac cggaaatatc cactaccgct
    24661 ttcagcacct gattgttttc cagctcctga gcaatttcgg ttgtcatatc cgaaccgaaa
    24721 acagcaattt ttccggcctg attttgatta cgtaccgctt ttaccgcgcc gagtgtcgca
    24781 ccgcccgact cccccataat ggcgttgaga tccggcgtgg agataatcag tttttcacca
    24841 acggaaatcg ctttatctaa aacagtccct tcctgattag cgacaatttg cgcgccggga
    24901 acgcgggatt ttaatacttc ttcaaatcct ttacgtcgct gcacacaaac ttcaaaggct
    24961 t
//
LOCUS       DADLSB010000046        24799 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.046, whole genome shotgun sequence.
ACCESSION   DADLSB010000046 DADLSB010000000
VERSION     DADLSB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.046"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(181..4797)
                     /locus_tag="JN331_001840"
                     /pseudo
     CDS             complement(181..4797)
                     /locus_tag="JN331_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4890..5537)
                     /gene="rnt"
                     /locus_tag="JN331_001841"
     CDS             complement(4890..5537)
                     /gene="rnt"
                     /locus_tag="JN331_001841"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY7260003.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5640..6047)
                     /gene="gloA"
                     /locus_tag="JN331_001842"
     CDS             complement(5640..6047)
                     /gene="gloA"
                     /locus_tag="JN331_001842"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY7260004.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6128..7213)
                     /gene="nemA"
                     /locus_tag="JN331_001843"
     CDS             complement(6128..7213)
                     /gene="nemA"
                     /locus_tag="JN331_001843"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY7260005.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISDSAQTKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQLWHTG
                     RISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIVNDF
                     RQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGDSVENRARLVLEVVDAG
                     IEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGE
                     PYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQ
                     LKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7250..7849)
                     /locus_tag="JN331_001844"
     CDS             complement(7250..7849)
                     /locus_tag="JN331_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7260006.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSSEPLENALAHVKNIIATPAV"
     gene            7952..8191
                     /locus_tag="JN331_001845"
     CDS             7952..8191
                     /locus_tag="JN331_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY7260007.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8240..9136
                     /locus_tag="JN331_001846"
     CDS             8240..9136
                     /locus_tag="JN331_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY7260008.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9217..9738
                     /gene="sodC"
                     /locus_tag="JN331_001847"
     CDS             9217..9738
                     /gene="sodC"
                     /locus_tag="JN331_001847"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY7260009.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9739..11751)
                     /locus_tag="JN331_001848"
                     /pseudo
     CDS             complement(9739..11751)
                     /locus_tag="JN331_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11751..12608)
                     /locus_tag="JN331_001849"
     CDS             complement(11751..12608)
                     /locus_tag="JN331_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7260010.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNVAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12611..12847)
                     /locus_tag="JN331_001850"
     CDS             complement(12611..12847)
                     /locus_tag="JN331_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY7260011.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13048..13482
                     /gene="slyA"
                     /locus_tag="JN331_001851"
     CDS             13048..13482
                     /gene="slyA"
                     /locus_tag="JN331_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY7260012.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13529..13996)
                     /gene="slyB"
                     /locus_tag="JN331_001852"
     CDS             complement(13529..13996)
                     /gene="slyB"
                     /locus_tag="JN331_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY7260013.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14270..15379
                     /gene="anmK"
                     /locus_tag="JN331_001853"
     CDS             14270..15379
                     /gene="anmK"
                     /locus_tag="JN331_001853"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY7260014.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            15477..15806
                     /gene="mliC"
                     /locus_tag="JN331_001854"
     CDS             15477..15806
                     /gene="mliC"
                     /locus_tag="JN331_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY7260015.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15865..16521
                     /gene="pdxH"
                     /locus_tag="JN331_001855"
     CDS             15865..16521
                     /gene="pdxH"
                     /locus_tag="JN331_001855"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY7260016.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16650..17924
                     /gene="tyrS"
                     /locus_tag="JN331_001856"
     CDS             16650..17924
                     /gene="tyrS"
                     /locus_tag="JN331_001856"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY7260017.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            17986..18846
                     /gene="pdxY"
                     /locus_tag="JN331_001857"
     CDS             17986..18846
                     /gene="pdxY"
                     /locus_tag="JN331_001857"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY7260018.1"
                     /translation="MKNILAIHSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(18890..19495)
                     /gene="gstA"
                     /locus_tag="JN331_001858"
     CDS             complement(18890..19495)
                     /gene="gstA"
                     /locus_tag="JN331_001858"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY7260019.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19601..21103)
                     /gene="dtpA"
                     /locus_tag="JN331_001859"
     CDS             complement(19601..21103)
                     /gene="dtpA"
                     /locus_tag="JN331_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY7260020.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21714..22349)
                     /gene="nth"
                     /locus_tag="JN331_001860"
     CDS             complement(21714..22349)
                     /gene="nth"
                     /locus_tag="JN331_001860"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY7260021.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22349..23044)
                     /locus_tag="JN331_001861"
     CDS             complement(22349..23044)
                     /locus_tag="JN331_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY7260022.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(23048..23668)
                     /gene="rsxG"
                     /locus_tag="JN331_001862"
     CDS             complement(23048..23668)
                     /gene="rsxG"
                     /locus_tag="JN331_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY7260023.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23672..24730)
                     /gene="rsxD"
                     /locus_tag="JN331_001863"
     CDS             complement(23672..24730)
                     /gene="rsxD"
                     /locus_tag="JN331_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY7260024.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         6150 a         6415 c         6463 g         5771 t
ORIGIN      
        1 atacagcgaa cgccgatgta aatggcgctc gtaacatttt agcggcgggg cacgccgttc
       61 ttgcctgtgg agagatggtg cagtcaggcc gctcgttgaa gcaggaaccc accgaaatga
      121 ttcaggcgac agcttgaacg tagcagggaa ccacgtcctt cagggcgtgg aggatgtcaa
      181 ctatccccaa tccagccctt gtggcgaact tgaaaatccc gcctcacgca gcagcgtaaa
      241 catcggcgtt tgccggaccg gaagatcatt cacttctgtt agcgtaaagc gcagccttgg
      301 ttcgcgacgc agtgcggtgg tcagcgcctg gaaaatctct ggggcgagta attcctcctt
      361 ttcctgccag accagcattt ttttgccgcc ttgcgccaga tagagtaaca atttgccgcc
      421 agaaacgacc accagcgcac ccgcccgacg cgttggtacc agcgttgccg gatgtgcagg
      481 ccaaggcaga agatttcccc acacattagc cggatcgttg gcggagagcg ccactggtgt
      541 atagtggcgt gtttgcgtgg cttgtgtcgc cagatcgcgc aatcggtcaa tagtcagacg
      601 ttcagcgaat tgcgcgccac ccagaccttc tacaaaacga cctcgcataa ttcgcccgga
      661 gtcttccata cttcgacaaa gtgtttgcat cgatggaaac ccgccaggga tattttcggc
      721 tatcaccgcc tgacgactga tgatgccgta gcggtcgagc atattttccg ccagcgctag
      781 catcctttcg gtatcgttta gtggctccac ctgcaataac gaccagcgtc cagccagatt
      841 tggtgtgttg taagataccc gcggcgagac gggttgtgca tagacaggac gcccacgacg
      901 agcccggtga ctgcggcgag ttgaggtgcg tgcgttggaa ctgctgcggg tgagggcgcg
      961 taacggtgcc caaatatcgc tggtaatgac gccttgccag accagcgccc ataacgcctc
     1021 ttgcagtgcc gaggaatcaa ccgattcgcc gattttatcg cgtatccgtt ggctgatttg
     1081 ttgtgcaaac caggctcctc cgtcagccag aacagcgatt atcgcttgct gcagcgccga
     1141 acgattcgcc tgatccgctt cggcgggagt gaacgattct gcggcatatt tctgtagatg
     1201 cagtgccacc aggccgtcat cttcacccag ctttttttgt cccgaccaga taaccgctcc
     1261 ggttgccagt aattcatcga gcatttctgg cgaatagtcg cgtacgcggg caggcaggat
     1321 ctggctttcc cagagtgagg cgggtaaacc gactccggca agctgttcga tcacccgcat
     1381 cacgccatct acgccctcat aaacgcctgg ctatgttgag gcaaagagcg ccgggctacc
     1441 atcggtggcg ggtaatacgc cctgacgttc caacagcaat cgcgcatagg tcgtggctgc
     1501 aacgggacgc gtcgcttctc tggcggcttg cagcgagcgc aaacgcagac gacgaaatac
     1561 ttcatcgctc acccagatgt cttgttgcag attcatcacc agaccctgtt cacgcagttg
     1621 ctgaagctgc tcttcggcaa tggcaatacc gagactaaac tcatgagcca gttgttcagc
     1681 cgtgaccaga gcatgagccc ggagatagcg cagaaagagg tcgcgaagcg ggtaactgac
     1741 tctatgtaaa taaatctctg gcaatgactc tggtagtcgt acgccgaggg catcacgcag
     1801 cctggcggca tcatccatac aggcaagacg ctcctgtccg ctaatcatcg ctgggaagat
     1861 tcgttttact gcaagaagat tttccagata cgacgcaacc tcttcactgc tgcctgtatg
     1921 ccgttgcgcc aggtcttcaa cggtcattgg ccccagttcg cgcagcaggt cgaacaatcc
     1981 ttcttcacct ttcgctcttc tgccaggagc cagtcgttgc aactcttctt ccacctggcg
     2041 aatgacctgc gggtcgagta attcccccgg atcgacctgt ccgagtagat tgcgcagtaa
     2101 ctcgctgtcc agcgacagta cggatgcccg gcgctctgcc agcggggcgt cgctctggta
     2161 cataaattcc gcgacatagc cgaacaataa acttgtggcg aaaggcgagg gcgtagtggt
     2221 cgttacatcg gatatttgaa tttcgccacc gttcagacga cgcatcaaac gttccagtgc
     2281 gggaagatca taaacatctt gcagacattc gcgtagggtt tcgagaatga ccggaaaatc
     2341 cggatatccc tgagcgattt ccagcaactg actggcgcgc agccgttgtt gccaaagcgg
     2401 ggtgcgatgg cccggcgtgc ggcccggcat taataatgcc cgcgcggcgc attcgcgaaa
     2461 acgggcggcg aaaagtgccg agctgcctac cgcctcgcgg acaatttgca gcaacttttc
     2521 tggttcaaac aaaaaaatcg cggcgtcggg caatttacca tcagtgtcag gaatgcgcgc
     2581 aacaatgccg tcatcactgg cgaccacaga cgcgtcagcg ccccataggg catgtattcg
     2641 cccggcaatc gccagcgccc agggttcatg cacccgtctt ccatagggag agtgcaaaat
     2701 aatacgccag tcgccaattt catcacggca ccgttcgagg accagatggc gactccccgg
     2761 aacgatgccc gtcgcgttgc gttgctcgtc aatcaacccc tgaatattgg cgttcgtatt
     2821 ttcttctgcc agccacgggg gaatagtccc ggaaaagaac gcgccatccg ccagtaaatg
     2881 aagaaaatcg ccgatcatct cgcctaattc agccggacgt ccgttacctt caccacgcca
     2941 gaaggggagc cgggcagaac gacccggagc aggagtcaca atcacctgat cgcgggtgat
     3001 ttgctggatc cgccatgagg tagcgccgag agtgataatg tcgttcaccc gcgactcata
     3061 taccatctcc tcatccagtt cacccacccg ccgcgaaccg gccttttctt caccttcggg
     3121 taataacacg ctatacatgc cacgatccgg aatggtgccg ccgctggtaa ccgccaacaa
     3181 ttgagcgcca ggtcgggcgg tcaatatccc ggtctccctg ttccagacca gtttggggcg
     3241 aaaagcagaa aaatcgccag agggatagcg cccggaaagc atatccagcg tggcgtcaaa
     3301 aacacgtctt ggcagatctt tccacggtgc ggcacggcgt acgcgggagt accattcgtc
     3361 tacctgtaat gcatccatcg ccgcggcggc aacggtttgc tgcgcaagga cgtcgagagg
     3421 attatgcggt ggtgtcaggt tttccagcct gcctgcgaac atacactcta caatgactgc
     3481 ggaatcgact aaatcacgcc gggtacgggg gaaaaacagc cctttagata caccgccaac
     3541 ctgatgtccg gcgcgaccaa tgcgttgtaa cccactggca acagaaagcg gcgttgccac
     3601 ctgaatcacc agatccaccg cgcccatatc aatccccagt tcaagactgg aggttgcgac
     3661 cacgcagcgt aattcacccg atttcagcgc ctgttcggtg attgctcgtt gttctttaga
     3721 gacggagccg tggtgtgagc gggcaataaa aacgtcgcta ctttgtacac ggttagaggt
     3781 tgcgccggag gtcgactcga aatgggccac atcaacggcg atagacgggg aacgctgtaa
     3841 gcgtgcggcg taaagctcat ttaatcgtgc cgtcagtttt tccgccagcc cgcgtgaatt
     3901 agtaaagaca atggtcgagc gatggcgcaa cacttcatca aggatacccg tttcaatata
     3961 tggccagatg gagccttccc ggccggcatg gctgtcttcg ccggtgccgc tggcgaccga
     4021 tgagacatca tccatattgg cgaccggtac gacaattcgt atctgcggat ggcgcattgc
     4081 gggcgggttg actaccgtaa ccgggcgatc gccaccaaga aatgctgcca catcgctggc
     4141 tgagcgcaca gtggcagaaa ggccaattcg ctgtgctgag gtgtggagca gcgcatcgag
     4201 ccgctccaga cttaacgcca gatgcgcacc acgtttactg cccgctaccg cgtggacttc
     4261 atcaataatt accgtttcga cgccgcgtag cgtttcgcgc gcgcgggagg tcagcatcag
     4321 atagagtgat tcgggtgtgg tgatcagaat atccggcgga ttacgggtga gtttgctgcg
     4381 ttcctgtgca ggcgtatcgc cagtacggat ccctacgcga agattgactt ccgtttcgcc
     4441 gcgccgccgc cgttcatcgg caataccctt caacgggatc tgcaagttgc gctgaacgtc
     4501 ggtgcccagg gcttttatcg gtgaaatata gaggatgcgt gaggttttac gcttatgcgc
     4561 ctcgcgggta tcttcgccgc cttcgcggaa gagccgatcg agggcgtaga gaaatgctgc
     4621 cagcgttttc ccggagccag tcggtgcaat caccagcgca tgttcgcctc gcgccgccac
     4681 atgccaggtt tgcgactgga cagcggtcgg ctgtttaaag gcgcgaagaa accagtcgcg
     4741 ggtcgccggt gaaaacacgt ccggcaggag cgatgaaggg tctggattat ctgccattaa
     4801 cgctattccc cgtgaatatc atgcttgatg cgtggaatag cctcactata gatgcctgaa
     4861 tcattgcatg tcatcaggca tcgactcgat tacacctctt cggcggcagg tagcggccag
     4921 cctcccagac gtttccagcg gttgacgatt tcacaaaaca gcacagcagt gcgttcggtg
     4981 tcgtacagcg cggagtgcgc ctgggtgctg tcgaagtcca tgccagcggt ctggcaagcc
     5041 tttgacaata cggtttgtcc gagtgccagc ccggccagtg cagcagtgtc aaaagtggcg
     5101 aaagggtgga acgggttacg tttcagcgag gcgcgttctg cggcggccat cataaagctg
     5161 tgatcaaaat tggcgttgtg cgccaccata atggcgcggt tacagccgct cgctttaata
     5221 cctttacgta caactttaaa aatttcgtgc agcgcctcgt attcgctgac cgcgccgcga
     5281 tcgggatcgt tcgggtcaat gccgttgaag gcgagggctt ctggttgcaa atttgcgccg
     5341 acaaatggtt cgacgtggaa atgtaatgtg gtgtccggca tcagccagcc ttgttcatcc
     5401 attttcaggg tgatggcggc aatctcaagc agcgcatcgg ttttggcgtt aaatccggct
     5461 gtttcaacat cgatcacaac aggataaaaa ccacgaaaac ggtcgcacag accggtaagt
     5521 tgagcgttat cggacatctg attctcttaa tacggataaa ttgcagcgcg cattatgaca
     5581 aatattactt gtcgatgcag taaagatgcg ggcgcgatga gttcacgccc ggcaggagat
     5641 tagttgccca gaccgcgacc ggcgtctttc tcttcgatta actcaatttt gtaaccgtcc
     5701 ggatcttcca caaacgcgat aaccgtagta ccgcctttta ccggacccgc ttcacgggtc
     5761 acgttacccc cgttttgacg gattttttcg cacgcttcag cggcgttatc tacgctaagc
     5821 gcgatgtgac cataagcagt gccgagttcg tatttatcca cgccccagtt gtaggtcagt
     5881 tcaatcaccg cttcttcggt ttccgggccg tagccaacaa acgccagtga gtatttgtat
     5941 tccgggtttt cgctggtacg cagcagtttc atgcccagca ctttggtata aaaatcgatg
     6001 gagcgttgca aatcgccaac gcgcagcatg gtatgaagaa gacgcatctt tttatcctca
     6061 aaatggctaa cagattcaaa atgttgtttt agtatagcgg cggctttacg ccgctcgcaa
     6121 tgttggatta caacgtcggg taatcggtat agccttccgc gccgccaccg tagaaacttt
     6181 cggcacgctg tgggttaagc tcagctttga gctgcaagcg ggcgaccaga tccgggttcg
     6241 caatccagtc acgaccaaat gccaccgcat caattaaccc tttgccgatc agcgtttcag
     6301 ctttttctac tgtgtatgca cctgcgccga taatcggacc gtggaaacgg gcgcgtactt
     6361 tttcgcggaa cgcatcagta tacggttcac cccccgccca atctggttct gacatatgca
     6421 gataagcaat gccgcgttta cccagttgtt caatcagata cagtgcatcg gcttcttcat
     6481 tcgggccgtt atctgtgttc tggaaagtac cgattggcga aacgcgaatg ccaatgcgat
     6541 cggcacccca ttcttcaatc ccggcatcga ctacttccag taccaaacgt gcgcgatttt
     6601 ccacgctgtc gccgtactga tcggtacgat ggtttgaaga aggggaaagg aactgatgca
     6661 gcaaataacc gtgagcagag tggagctcta ccagatcaaa accggcttca cgcgcgttag
     6721 caatggcctg acggaaatca ttgacgatac ccggaatctc ttccagttca agcgcacgcg
     6781 gcatggatgt ttcaacgcgg atcgcctgac cattttcatc gcgcagagaa gtacgtgttc
     6841 ccgcgctaag tgctgaaggc gctaccggtg cctgaccgcc aggttgcagg ctggcgtgag
     6901 aaatgcgtcc ggtgtgccac agctgcaagg ccatatgacc attttcagca tgaacgccag
     6961 cggtgatttt tttccatgcg gcaatttgct ccggactatg gatgccaggc gcacctgcat
     7021 atccttttgt ctgggcagaa tcactaataa tcaaaccggc actggcacgt tggcgatagt
     7081 attccgccat caacggggta ggaatgtcac ccggttcaat actgcgcaga cgcgtcagcg
     7141 gtgccataaa aatacggttt gccgccgtga tcgcgcccac tttcagtggg gaatacagtt
     7201 tttcagatga cataaaggct cccggtcgtc tggtgataaa aaatgcctgc taaacggcag
     7261 gcgtcgcaat aatgtttttt acatgggcca gcgcgttttc cagtggctcg gaactgcgcg
     7321 aaattttggc ctgcagattc gcgccaagcc atagtgcgta aagcacttgt gcctgttgta
     7381 gcggttcgcc acaaaaggtt aaacaatggt tctcacggcc attttccaga gcctgcgaga
     7441 gcagggcgat cacgccgcga gcacctttat ccatcgcgct gcgcatatct tctgacagat
     7501 cgcacacttc ggcagagagt tttactgtca ggcaaccact gatggttcca tgttggcaaa
     7561 actggttcag tgtttgctgg taataagcca gtatgcggtc gcggtagtta ccttcgccgg
     7621 attgcagcaa ctcagtcagt cgctggtgat atgcggcgta atgacgctca agcatggcaa
     7681 cgccaaacgc ttctttagag cgaaagtagt gatagaagga ccctttcggc acttcagcgg
     7741 tttttagtaa ttcgcttagc cccatcccgg tgaatccacg ttgcaggcaa agttgctcgc
     7801 ccgtcgccaa gagatgttcg cgagtatcat gttcggtgtg tttgttcatg cgttggagtg
     7861 tagtagacca gtcggtctag tgcaagcaaa gttgctactt tggacattaa cagcttcaat
     7921 aatggtaata atcttatctg aggagggcga ggtggcggag caattagagt tctttcccgt
     7981 ccagagcccg tgccggggaa tttgccagtc tgacgaacgc gggttttgtc gcggctgttt
     8041 tcgtagtcgg gatgagcgtt ttaactggaa taaaatgagc gatggcgaaa aacaagaggt
     8101 gctgcgcctt tgtcggcagc gattaatgcg caaattacgc gcaaataagc cagcgtcatc
     8161 agacgaaccg gagcaaccat cactcttttg aacctaatct tgcgtatact cctctcatat
     8221 ctgataagag gaagcgatta tggttcagcg tattactatt gcgccgcaag gcccggagtt
     8281 ttcccgtttt gtgatgggct actggcgatt gatggactgg aatatgtccg cccgccagct
     8341 ggtcagtttt attgaagagc atctggatct cggcgtgacc accgtggacc atgctgatat
     8401 ttatggtggc tatcagtgcg aagcggcgtt tggcgaggca ctgaaactgg cacctcacct
     8461 gcgtgaacgg atggaaatcg tcagtaaatg cggtatcgcg acgaccgcgc gtgaagaaaa
     8521 cgtcattggt cattacatca ctgaccgcaa tcacatcatt aagagcgccg aacagtcgct
     8581 aattaatctc gcgaccgatc atctggatct gctgttaatc caccgaccag acccgttaat
     8641 ggatgccgat gaagtggcgg acgcgttcaa acatctgcat cagagcggca aagtgcgtca
     8701 ttttggcgta tcgaacttta cgcctgcgca atttgccctg ttgcaatcac gtctgccgtt
     8761 tacccttgcc actaatcagg tggaaatatc cccggtgcat cagccgttac tgctggatgg
     8821 cacgctcgac caactacaac aactgcgtgt tcgtccgatg gcgtggtcct gccttggtgg
     8881 tggtcgtctg tttaatgatg attatttcca gccgctgcgt gatgaactgg ctgtggtggc
     8941 ggaggagtta aacgcgggct cgattgaaca ggtggtttac gcctgggtat tacgtttacc
     9001 gtcgcagccg ctgccaatta tcggctcagg taaaattgag cgcgtacggg cagctgtcga
     9061 agcagaaaca ctgaaaatga cccgtcaaca atggtttcgt atccgtaaag ctgcactggg
     9121 gtatgacgta ccgtaagcac ttttaggaat agccgccgtt caaaaatgtg tcactggttt
     9181 acacttattc aggaatgcac aatgaacgga ggtcctatga aacgttttag tctggccatt
     9241 ctggcgctgg ttgttgcgac cggcgcacaa gctgccagtg aaaaagtcga gatgaacctc
     9301 gtcacgtcgc aaggggttgg gcagtcaatt ggtagcgtca ccattactga aaccgataaa
     9361 ggtctggagt tttcacccga tctgaaagca ttaccccccg gtgaacatgg cttccatatt
     9421 catgccaaag gaagctgcca gccagccacc aaagatggca aagccagcgc cgcggaatcc
     9481 gcaggcgggc atcttgatcc acaaaatacc ggtaaacatg aagggccaga aggtaccggg
     9541 catttaggcg atctgcctgc actggtcgtc aataatgacg gcaaagctac cgatgccgtc
     9601 atcgcgcctc gtctgaaatc actggatgaa atcaaagaca aggcgctgat ggtccacgtt
     9661 ggcggcgata atatgtccga tcaacctaaa ccgctgggcg gtggcggtga acgctatgcc
     9721 tgtggtgtaa ttaagtaatt acgaggccag cgtaccttgc ggtggtgctt gctcaagttg
     9781 cgaaagcgag cagtacagcc gccagacaat tgccgccagc tcacgggcgg caggttgatg
     9841 atgacgggca aggctgtcgc aaatccgctg taattcttca agtgtggccg ccagtgattt
     9901 ttgctgaacg ccacgctcac tcatcactcc ccgcaacagt gaaatacagt tatcccgtac
     9961 tcgcgataac ggatcggaac gcgattccca gtcgcgcaat tgccagacaa catgagaaca
    10021 gttcagcagc actacacccc agcgtaataa ccaacggcga gccagcgcat cctggctgtt
    10081 actcaactga ctgatgtgat gataagtgag cgattcaaat tcgctttcac tcagtgttgg
    10141 atggcggctt agctgatcga caaaatcccg gcgcagcgcg cgaatatggc ggcggctttt
    10201 acgagcatcc gatcctggac gcagaatggc gaacgctagc caagccagcg caacgccaac
    10261 gatttttgcc agattatcgt taagaaaatc agcaaaatca tacaccgggg gattagtgac
    10321 agcgataaac gaacccataa aaacaatcag ttgcccccac agtgcggcaa atttgggcat
    10381 ctgcaattta agaagctgca ttgtcgccag cagtggaaag agaaacagta aaaattgcca
    10441 cagatcgcta atctggacca tcaggccaaa tttgaccaca aagctgaata gcgaaagtaa
    10501 caccagcgtg cgcatcagca gtgacaacga cttaaacggt gctgcgacgg cggagtagag
    10561 tacgcagcta attgctgcca gcgttaatgc attggcaccg gcatcccatt gcgatgcaat
    10621 actccatgcg ccaatcatca tcaacgtaca aaatgtacgc agcccgctcc acatagcttc
    10681 ggcgttgtcg gtatgacgag ccagagcgga tgtccgtgcg agtctggcat gatcatctac
    10741 accgctttgc agacgatgta attcctgact actttgcaga taaaggcggc aaaaataacg
    10801 tagtcgctgc cagaaggcga cgtgccggta gtcggcgacg ttggtcgggc gtagcggggc
    10861 gataatacgt gcgacggtgt aagcatctgt ttgcgaactg gcgagcgccg tcagcaattg
    10921 ttcgagaatt tctcgcgtgg cacttggcgg tgagggccag ttgagcaaca tacgtcgcag
    10981 gctggagatg acactggtca tacgtaattg ctggtggagt agcgcattaa ggcgcgcgtt
    11041 ttgctggcga aaacgatagt ggctccagaa agcctggata cgcagcaaat tcatggtcag
    11101 tatctgccca atcaccccct catgtgctgc acgaatggca tcgtttgttt caggctgcca
    11161 gagtaaactg gcatgttcca gtaatcgggc gtgcatgttt ttcaatgcag ttaaaagggc
    11221 agtagcatcg gaactgctag gcaggatcat catcatcatg ccgccgcaca aaataccgac
    11281 tatcacctcg caaacgcgcg cctgagcgat atcccacagc tggctggcct cagtaatatt
    11341 aaccatcgga aaggcgatga tggcagccgt gtagcccgcc agttgaaatg catacgcgac
    11401 gttattcgtg aagtgcgcac aggcctaggt acaaaagcca agccacgccg acatgctcaa
    11461 tagaaaaaac cacggctcat tgagcgtatg tccggcaaga agcagtgccg caatggctcc
    11521 cagtaaactg ccagcgatgc gtccgaggct tttgctgatg acaccgccaa cggtgggaaa
    11581 gctaaccact gcagccgagg tcatcgccca atagggttca tccagattca aataataggc
    11641 aaccgtcagc gccagacaca tggcaatggt attgcgtaac gcataacgcc attgcgccag
    11701 cgtggccctg aaccagggca aattgcgcaa ggaccatgac gatgcgttca ttattgacct
    11761 acggcaatgc tacaggtggt gcccgacacc agcgtgacat cgcctggaac tttatccagc
    11821 gcaaagcgga cgggaacgcg ttgggcgaga cgaacccagg ggacgttagg tttcacatcc
    11881 ggaataaggc ttgagtcact ttcaacgctc tgatcataaa tcgcccgacc gatactggaa
    11941 acgtgaccct gtaacgtttt gttatcacta tagagggtaa tttgtgctgg tgcaccttcg
    12001 cggatatggc gcaacttggt ttcttcaaaa taaccaatga cgtaaaacga gtggctgtcg
    12061 acaagggcaa acagcggttt cccggtgtca gcgtaatcgc cgatgcgggt agtgaggtta
    12121 gtcacccatc cactcaccgg ggcgcgaatt tccgtttgcg ccagttgcca ttgtgtctgt
    12181 ttcagcgtcg cctgtgcgac attaacactg gcctgcatcg ctttaacatt gaggttagcg
    12241 gtatcgagtt cttcggcaaa aataaaattt tgtgagagat gacgacgacg attcgcttcg
    12301 ttattggctt ttgcgaggtc agattgcgct ttcgccagct gagcctgtgc gttcagctcg
    12361 gcgatctgaa agggcgtttt atcgatcgtg agcagaagtt cccccgcgtt aaccagttgg
    12421 ttgtctttta tattcagctc aacaatacgt cccgacacct gtggggtgat agaaacctgt
    12481 tctgcgcgta ttttcccgtc gcgtgtccac ggagattgca tgtaataatt ccacagccac
    12541 catccggcaa gaaccgcaac tatcgctaca atgattgttg agaaatactt aattgtttta
    12601 attgacatag ttaccacgca atcagtattg ccagagcaag gcaaacgcaa atcgcaaaca
    12661 gcgataaatc cattaacaag ggatgccaga tgtcaccggc gtagatccag ccacgaagca
    12721 ggcggtgtac gacaagccag atgacgaatc caaacgcgaa tgctttgaaa aacggaggaa
    12781 aatagacgga cgcaccgaac accagatctt gtaagggcaa tcctgttgca ttgagcataa
    12841 acttcacaaa gtagattcct ttacgaccga atatattgcg ttagattaat aaatattctt
    12901 taagtgcgaa aaatttacgc gcaatttctg aaaaatatgt ctgacggtaa ccaaatgcag
    12961 caatacattt gttttagcaa tacaattgct gcacactatt ctaaaagccg cataatatct
    13021 tagcaagcta attataagga gatgaaattg gaatcgccac taggttctga tctggcacgg
    13081 ttggtgcgca tatggcgtgc tctgatagac catcgcctga aaccgctgga gttaacacaa
    13141 acccattggg ttacgttaca caatatccat cagttacctc cagaccagtc gcaaattcaa
    13201 ctggcaaaag cgattggcat cgagcagcca tcactggtcc gtactctgga ccaactggaa
    13261 gaaaaagggt taatttcgcg tcaaacttgt gccagcgatc gtcgggctaa acgtattaaa
    13321 ctgacggaaa aggcagagcc actgatcagc gaaatggaag ctgttattaa caaaacccgc
    13381 gcggaaatat tacatggcat ctccgcagag gaactggagc aactgattac gctcatcgca
    13441 aaacttgagc ataatatcat tgagttacag gccaaagggt gaaatgaagg gggcgtgtgg
    13501 tcagtaacct gaccacacgc aagcttattt agcgcggaga aacggtcacc tgactgccat
    13561 tgctggccag tacgacacgt tggcccggag agaaacgagt gttgccttgt ttctgtacca
    13621 ccatgatggt attaccatcg tctttacgaa tttccagctc gacaccctgc gttttgttca
    13681 ttgcactctg tacgccctga ccagctacgc cacctgcaac agcgcctgct gcagtagcca
    13741 gagaacgccc ggttccgcca ccaacagtat tccccaggaa accaccaaga acagcaccgc
    13801 caattgcacc gataacgttg gaatcatcac cgccctgaat ctgtaccgga cgtacgttaa
    13861 cgatggtgcc atagctgaca ttctgtactt gtttcgcttc agaagcggta taaacatccc
    13921 ctgacagggt gtcgttatta acacaaccga caagagacag acctaccatt gaaacaacca
    13981 atacgcgttt aatcattgaa aaatctcctg ttcaccatga aacgccacgc aagcatccct
    14041 catggtcaaa gtatatggca tatgtgaatc catggttaca tgttctacca aatatgagca
    14101 aatcctaatg aaagtaggat caaccaatta taaacaaaac attcatgatg attattgatt
    14161 tgaagataat gttttactac caacggtaaa aatcggtcat tccgcatggt attcacgaaa
    14221 agtttatgtt tgagtggtgc gcaaacgtca gattgaaaaa ggatgaacta tgaaatcggg
    14281 ccgctttatt ggcgttatgt caggcaccag ccttgatggt gttgatgttg tgttggcgac
    14341 aattgatgaa caccgggtcg cacagctggc aagcttgagc tggccgatcc cggtgtctct
    14401 gaaacaggcc gtactggata tttgccaggg ccagcagctt acactttcgc agtttggaca
    14461 gcttgatact caactcgggc gactttttgc tgatgcggtc aatgccttgc ttaaggaaca
    14521 aaacctgcag gcgagagata tagttgcgat cggctgtcac ggtcaaaccg tctggcatga
    14581 accgacgggc gtggcaccac acactttaca gattggcgat aacaatcaaa ttgtggcacg
    14641 caccggaatt acggttgtcg gtgattttcg ccgtcgcgat attgccttgg gaggacaagg
    14701 cgcaccgttg gtacctgcgt tccatcatgc cttgctggct cacccaaccg agcgacgaat
    14761 ggtgctcaat attggcggca tcgccaatct gtcactgctc attcctgggc agccggttgg
    14821 cggctacgat accggtcctg gtaacatgct gatggatgcc tggatctggc gtcaggcagg
    14881 taaaccttac gataaagatg ccgagtgggc acgggcgggt aaagttattc tcccactgct
    14941 gcaaaatatg ctcagcgacc cgtatttctc gcaacctgca ccgaaaagca ccggacgcga
    15001 atactttaac tacggctggc tggagcgcca tttacgccat tttccgggtg ttgatccccg
    15061 tgatgttcag gccacactgg cagaactcac cgccgtgacc atttctgaac aagttttgtt
    15121 gagcggtggc tgcgaacgat tgatggtatg tggtggaggt agtcgtaatc cgctactcat
    15181 ggcgcgtctg gcggcattac tgctaggcac agaagtcacc accaccgatg ccgttggcat
    15241 tagtggcgat gacatggaag cattggcttt cgcctggctt gcctggcgga cgctggcggg
    15301 attaccagga aatctgcctt ccgtcactgg cgcaagccag gagacggtac tgggggctat
    15361 tttccccgcc aactcgtgat atcagaatta actgaatttt ttcttatcgt gtctgccgtt
    15421 acacttggaa gattaaggag ggtatttgcc ctccggacta ggaaagtctt cgggatatga
    15481 ctatgaaaaa actgttaatt atcattttgc cggtccttct ctccggttgt agcgccttta
    15541 atcaactggt tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt
    15601 tgacggtcaa actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac
    15661 tgcatctcaa acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt
    15721 tctggtcgaa aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact
    15781 gtcagttaca aaatccacag cgttgagatt tttccaaggg cggcgcacaa tagcgtcacc
    15841 cactgacaat ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat
    15901 ctgcgccgtg aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta
    15961 accctttttg aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg
    16021 atggtggtcg ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa
    16081 cattacgacg aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa
    16141 attgaaaata atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg
    16201 atggtgatcg gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc
    16261 cgcccgcgtg atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc
    16321 cgcggtatcc ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg
    16381 ccattgccga gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag
    16441 ggcggtgagc atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag
    16501 attgatcgtc ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg
    16561 attctggcac tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag
    16621 gtgcagtcgt tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc
    16681 aagagcgggg gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc
    16741 aaggcccgat cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc
    16801 atcttgttcc attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc
    16861 tggtaggcgg cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc
    16921 tgaacaccga agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt
    16981 tcctcgattt cgactgcgga gaaaactctg ctatcgcggc gaacaactat gactggttcg
    17041 gcaatatgaa tgtgctgacc ttcctgcgcg atataggcaa acacttctcc gttaaccaga
    17101 tgatcaacaa agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca
    17161 ctgagttttc ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg
    17221 atgtggtgct gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc
    17281 tgacccgtcg tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag
    17341 cagacggcac caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa
    17401 ccagcccgta caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct
    17461 tcctgaagtt cttcaccttt atgagcattg aagagatcaa cgccctggaa gaagaagata
    17521 aaaacagcgg taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg
    17581 ttcacggtga agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt
    17641 ctttgagtgc gctgagtgaa gcggacttcg aacagttggc gcaggacggc gtaccgatgg
    17701 ttgagatgga aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt
    17761 cccgtggtca ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac
    17821 agtccgatcc tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac
    17881 tgcgtcgcgg caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag
    17941 ggggagtgag aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc
    18001 gctatccact ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg
    18061 cgccgcctgg gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa
    18121 tacggcaaat ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc
    18181 attgccgcca ttgataaatt acacacctgt gatgccgtct taagtggcta tctgggatcg
    18241 gcggagcagg gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag
    18301 gcgaaatatt tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg
    18361 ggtgtcgcag agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat
    18421 ctggttgagc tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg
    18481 gcagcgcgcg aactcattgc gcaagggcca caaattgtgt tggttaaaca cctggcgcga
    18541 gctggctaca gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agcctggcat
    18601 atcagccgtc cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg
    18661 agcggtttac tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat
    18721 gtgaccgctg cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg
    18781 caagtggtgg ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag
    18841 ctctgaaata tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc
    18901 cttccgctga cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg
    18961 caatgtgctc taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa
    19021 acagataggc atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg
    19081 cctcgttcac atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt
    19141 ccggtgtatc tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat
    19201 tcagccattc gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt
    19261 cggggacgct gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat
    19321 cgtccagcag caatgcaggc acctgcccct tagggttaac ggcaaagtaa tcgtcaccgt
    19381 tttcgagacg ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac
    19441 gcagggtgat atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta
    19501 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct
    19561 aacgaaaagt tagcggcttg taaaagagtt tccctgaatt ttacgctaca gcagctttcg
    19621 ccgctttgtc tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca
    19681 tcagtactgc aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt
    19741 ccagtgacat cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac
    19801 caccaatcag gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca
    19861 gacgctgcgg aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc
    19921 cgatgctctg caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag
    19981 acgcgaattt cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga
    20041 ttgcaaactt ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa
    20101 tcggactacc gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg
    20161 ccagacccag aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca
    20221 tctggctgta cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca
    20281 ttttacgacg cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac
    20341 cgaaggcaac aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg
    20401 caacagcgat cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg
    20461 gttcaaagtc tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa
    20521 cgatagtgat cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg
    20581 cggccagcca cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt
    20641 acatggtgaa tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag
    20701 aagacgggtt agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa
    20761 caataccggc gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag
    20821 cgccgagcat aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag
    20881 cgaccagacc ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag
    20941 acatacccag ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa
    21001 aacgttccca taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt
    21061 tcaaactgac gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta
    21121 catcccatat taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt
    21181 tatagtggga ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt
    21241 tgtaataatc tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc
    21301 cagcgttaaa tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat
    21361 gatggcttga cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt
    21421 tctgaaaaat agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa
    21481 atcattttaa cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca
    21541 cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc
    21601 aaacggatta cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt
    21661 aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg
    21721 tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg
    21781 cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac
    21841 tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca
    21901 aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg
    21961 aatgcagtgt ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc
    22021 gcagcacgat cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg
    22081 atgatatttt ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct
    22141 tcaacgccca gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc
    22201 gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca
    22261 aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg
    22321 aggatctcca gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg
    22381 cacgttcggc agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc
    22441 ctgccagcat cagtcccaag ccaataaatg cacctggtgg tagcatcgcc agcaggaaag
    22501 gggagtcggt gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat
    22561 ctgcaccgtc aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca
    22621 tggcgcaggt tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt
    22681 ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa
    22741 taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag
    22801 cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc
    22861 agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc
    22921 ctaaacccag agcgttagtg gcagtggacg tgaccgccaa tagcggacaa aggccgagca
    22981 gttggaccag tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc
    23041 tcacgcttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt
    23101 acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga
    23161 actggtcgaa atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga
    23221 ttttttttcc cgcaaaatgg gtgatccagt cagaaaggcg caattcgatt ttatcgccaa
    23281 gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg
    23341 ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg
    23401 cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact
    23461 ctggcgcagt taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca
    23521 gcacctgatc aaataacgcc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg
    23581 tcatctggtt gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa
    23641 tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg
    23701 gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca
    23761 cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc
    23821 cgaacatcag ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga
    23881 atgcgccgag catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg
    23941 gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa
    24001 tatgccagcg aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat
    24061 ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct
    24121 gttcaaccga atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac
    24181 taatgccatc aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc
    24241 tgaaaataac ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg
    24301 gtaaccagct ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg
    24361 ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa
    24421 acaccgtacc cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca
    24481 ataagcctgt cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta
    24541 gtttgagtac gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga
    24601 gagtacccca accaaaaaac cacagttgcg ctgcgattcc tggcacggct gcgagcaaca
    24661 ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc
    24721 tgaataccat ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc
    24781 gggcaatagc cgcggcaac
//
LOCUS       DADLSB010000047        24589 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.047, whole genome shotgun sequence.
ACCESSION   DADLSB010000047 DADLSB010000000
VERSION     DADLSB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.047"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(58..1083)
                     /gene="tdh"
                     /locus_tag="JN331_001864"
     CDS             complement(58..1083)
                     /gene="tdh"
                     /locus_tag="JN331_001864"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY7260025.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1093..2289)
                     /gene="kbl"
                     /locus_tag="JN331_001865"
     CDS             complement(1093..2289)
                     /gene="kbl"
                     /locus_tag="JN331_001865"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY7260026.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2564..3421)
                     /gene="yibB"
                     /locus_tag="JN331_001866"
     CDS             complement(2564..3421)
                     /gene="yibB"
                     /locus_tag="JN331_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY7260027.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3725..4657
                     /gene="rfaD"
                     /locus_tag="JN331_001867"
     CDS             3725..4657
                     /gene="rfaD"
                     /locus_tag="JN331_001867"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY7260028.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4667..5713
                     /gene="rfaF"
                     /locus_tag="JN331_001868"
     CDS             4667..5713
                     /gene="rfaF"
                     /locus_tag="JN331_001868"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY7260029.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5717..6709
                     /gene="rfaC"
                     /locus_tag="JN331_001869"
     CDS             5717..6709
                     /gene="rfaC"
                     /locus_tag="JN331_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY7260030.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGVAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPRLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6706..7914
                     /gene="rfaL"
                     /locus_tag="JN331_001870"
     CDS             6706..7914
                     /gene="rfaL"
                     /locus_tag="JN331_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY7260031.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(7950..9092)
                     /locus_tag="JN331_001871"
     CDS             complement(7950..9092)
                     /locus_tag="JN331_001871"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY7260032.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9101..10114)
                     /locus_tag="JN331_001872"
     CDS             complement(9101..10114)
                     /locus_tag="JN331_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAY7260033.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKELPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDEAIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10139..10846)
                     /gene="rfaY"
                     /locus_tag="JN331_001873"
     CDS             complement(10139..10846)
                     /gene="rfaY"
                     /locus_tag="JN331_001873"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY7260034.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAAKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10872..11879)
                     /gene="waaO"
                     /locus_tag="JN331_001874"
                     /gene_synonym="rfaI"
     CDS             complement(10872..11879)
                     /gene="waaO"
                     /locus_tag="JN331_001874"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY7260035.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNLRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(11922..12719)
                     /gene="rfaP"
                     /locus_tag="JN331_001875"
     CDS             complement(11922..12719)
                     /gene="rfaP"
                     /locus_tag="JN331_001875"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY7260036.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12712..13836)
                     /locus_tag="JN331_001876"
     CDS             complement(12712..13836)
                     /locus_tag="JN331_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY7260037.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQSKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13833..14891)
                     /gene="rfaQ"
                     /locus_tag="JN331_001877"
     CDS             complement(13833..14891)
                     /gene="rfaQ"
                     /locus_tag="JN331_001877"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY7260038.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYLDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQDGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            15304..16581
                     /gene="waaA"
                     /locus_tag="JN331_001878"
     CDS             15304..16581
                     /gene="waaA"
                     /locus_tag="JN331_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY7260039.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            16589..17068
                     /gene="coaD"
                     /locus_tag="JN331_001879"
     CDS             16589..17068
                     /gene="coaD"
                     /locus_tag="JN331_001879"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY7260040.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(17107..17916)
                     /gene="mutM"
                     /locus_tag="JN331_001880"
     CDS             complement(17107..17916)
                     /gene="mutM"
                     /locus_tag="JN331_001880"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY7260041.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLLLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(18014..18181)
                     /gene="rpmG"
                     /locus_tag="JN331_001881"
     CDS             complement(18014..18181)
                     /gene="rpmG"
                     /locus_tag="JN331_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY7260042.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(18202..18438)
                     /gene="rpmB"
                     /locus_tag="JN331_001882"
     CDS             complement(18202..18438)
                     /gene="rpmB"
                     /locus_tag="JN331_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY7260043.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(18655..19323)
                     /locus_tag="JN331_001883"
     CDS             complement(18655..19323)
                     /locus_tag="JN331_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAY7260044.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            19495..20715
                     /gene="coaBC"
                     /locus_tag="JN331_001884"
     CDS             19495..20715
                     /gene="coaBC"
                     /locus_tag="JN331_001884"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY7260045.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            20693..21151
                     /gene="dut"
                     /locus_tag="JN331_001885"
     CDS             20693..21151
                     /gene="dut"
                     /locus_tag="JN331_001885"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY7260046.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     QGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            21258..21854
                     /gene="slmA"
                     /locus_tag="JN331_001886"
     CDS             21258..21854
                     /gene="slmA"
                     /locus_tag="JN331_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY7260047.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(21891..22532)
                     /gene="pyrE"
                     /locus_tag="JN331_001887"
     CDS             complement(21891..22532)
                     /gene="pyrE"
                     /locus_tag="JN331_001887"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY7260048.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22598..23314)
                     /gene="rph"
                     /locus_tag="JN331_001888"
     CDS             complement(22598..23314)
                     /gene="rph"
                     /locus_tag="JN331_001888"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY7260049.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            23441..24304
                     /locus_tag="JN331_001889"
     CDS             23441..24304
                     /locus_tag="JN331_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY7260050.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEIMQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
BASE COUNT         6556 a         5963 c         5461 g         6609 t
ORIGIN      
        1 acataattaa cagattatcc tcagataaaa gggctggaat accagccctt gttcgtgtta
       61 atcccagctc agaataactt tcccggactg gcccgaacgc atagcgtcaa agcccttctg
      121 gaaatcatcg atagagaaac gatgggtaat gatcggcgag agatcgaggc cagactgaat
      181 cagcgccgcc atcttgtacc aggtttcaaa catctcacga ccgtaaatgc ctttaatgaa
      241 caagccttta aagatcactt tggtccagtc gatagacata tcagacggtg gaatacccag
      301 catcgcaata cgaccgccgt ggttcatggt atcgagcatg gtacgaaacg ctggcggcgc
      361 gccggacatt tccagaccga catcaaagcc ttcggtcatg cccagttcag ccatcacatc
      421 attaaggttt tctttcgcga cgttaaccgc acgggtgata cccattttgc gcgccagctc
      481 aaggcggtat tcgttaacat cggtgatcac cacattgcgt gcaccaacgt gtttcgccac
      541 cgccgcagcc ataataccta tcggacctgc accagaaacc agcacatctt cgcccaccag
      601 atcgaacgac agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc
      661 gtcggagata ttgtcgggga ttttgaaagc gttgaacgcc ggaatcacca gatattcggc
      721 gaagcagccc ggacggttaa caccgacacc aatggtgttg cggcacagat gggtacgacc
      781 gccacgacag ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc
      841 gattttgaag cctttcactt cctgaccaat acctaccact tcaccgacat attcatggcc
      901 cacgaccatc ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac
      961 gtcagtcccg cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc
     1021 cggtacaggg acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt
     1081 catctcacat cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac
     1141 tgcacgcgta atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg
     1201 acctttcgga acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc
     1261 tcgggcaaat ttctgcgcca ctaccgcatc accaagcatg accggaataa tggcgtgatc
     1321 ggctcccgcc agggtaaagc ccgccgccga catttgctca cggaactgac gcgcgttcgc
     1381 ccacagacgg tcacgcaact cgctacccgc ttcaaccatc tccagcactt tgatggaagc
     1441 ggcgacaatc gctggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag
     1501 ccactcaacc acttctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt
     1561 accgagcgtg ccggtgatga tgtcgacacg ccccatcaca tcgcagtatt catgggaacc
     1621 acgaccattt tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca ccagggcatc
     1681 gtatttatct gccagatcgc aaacgccctt cagattggca atcacgccgt ccatcgagaa
     1741 cacaccatcg gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg
     1801 agcttcaagc tcctgcatat cgttgttggc atagcgatag cgtttagctt tacacagacg
     1861 cacaccatca ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcgcc
     1921 cagcagcgtt tcaaacaggc caccgttagc atcaaagcag gaagagtaga gaatcgcatc
     1981 ttccatcccc aggaatgccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc
     2041 gcaaataaaa cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgctgc
     2101 cgcaatcaga tccggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac
     2161 gtggcttcca tcagccacgg tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc
     2221 tttaaacaac ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc
     2281 tccacgcatt gcgattctcc agacagggca aattccagca catattaccc aaacttatag
     2341 gtcgcgacga gatgatgcgt taacacttct gcaaaattca ggataaataa cgtgtatctt
     2401 gatgtctctc aacacctaca gatagtagaa aaactgtaca gttttggctg agcataatga
     2461 tgagtgattt tactgcattg atataaaaag aaaaaatgat gccaaagcat catttttcct
     2521 atctcagttg gcatcaaaac gctacccact aatggaggga aacctatttt cttgaaagga
     2581 aaattctcag cgcctgcatt tttgccccta aagtgttact cctgaagcag cgaaacaaat
     2641 cgaaccattt tcctctcccc agatagttta ggttgaagag atcggaacgt ttgtaatagc
     2701 acatgacaaa aatgccctgg tcatcgtcca caatgttatt gtttagtgtt attttttgac
     2761 tttccagaac taatttatag aattctttcc atttatgctg tgaaccaaca attgcgcccc
     2821 caataatata tacatgattg ccaatcatga aatcaaaagc ctgttgttga gatgtaacag
     2881 taagcccttt ttttatagtg aaaagatgca tcttattttc atcaaatggg aaatcccaaa
     2941 ttttcaatcc tcgagttaca tttggcttac ggcaatagcc aaaatctatc cacgcaacaa
     3001 gaggcgtttt cactaaaccc atgttgatag ctttatttac aaaataggcc ttaaggttac
     3061 ataccaaaac atactctggt gaccagtact ctgggttttt taattgtcga ggctcaagtc
     3121 tatttgtaaa cgactcatct ttttgaattt tttcgatacg acttctgata tatctaaatt
     3181 tcttttttat atcgataaca ataaccgttg ttggttttcc gttgcgaatc gcttcaatcc
     3241 ttggtttcaa atcgggagaa gtgaaaataa tcatctcatt ttcaagtgcg gccagacgct
     3301 caaagtaact aaaataaaca tcaactgaac gagctagttt ttcacggaac cctttattag
     3361 cagtccaatc ccctctacca atgtcaaaat atgcagtgat aattgtggtg gatgatttca
     3421 ttactaataa gaccattaat ataaaaaaag aattatatca taaatattaa cagaatgtat
     3481 actgtatatt atccattcaa atgattccgt tatttatcat acatcggaat attgatacta
     3541 aagcactatt cacatgcaaa accaacatcc gccatgaagg actagctaaa acccaaacta
     3601 gtttgttgca attagcatcc ttgcacctct atgtaaaggg ctgaagggat tcggatgtga
     3661 tggtatgatt acagacattc gtgtctgaga ttgtctctga ctccataatt cgaaggttac
     3721 agttatgatc atcgttaccg gcggcgcggg ctttatcggc agcaacatcg ttaaggcctt
     3781 gaatgataaa ggcatcaccg atattctggt ggtggacaac ctgaaagacg gcaccaagtt
     3841 tgtgaacctg gtggatctgg atatcgcgga ctatatggat aaggaagact tcctgatcca
     3901 gattatggct ggcgaagagt tcggtgatgt cgaagcgatt ttccacgaag gcgcatgctc
     3961 ttccaccacc gagtgggatg gcaagtatat gatggataac aactatcaat actccaaaga
     4021 gctgctgcac tactgtctgg agcgcgaaat cccgttcctg tatgcctctt ccgcagccac
     4081 ctacggcgga cgcacctccg actttattga atcccgcgag tacgaaaaac cgttgaatgt
     4141 ctacggttac tcaaaattcc tgtttgatga atatgttcgt caaatcctgc cagaagcgaa
     4201 ctcgcagatt gttggcttcc gctatttcaa cgtttatgga ccgcgtgaag gccataaagg
     4261 cagcatggcg agcgtcgctt tccatctcaa cacccagctt aataacggtg aatcaccgaa
     4321 gctgttcgaa ggtagcgaga acttcaaacg tgacttcgtc tatgtgggcg acgtggcaga
     4381 tgtaaacctg tggttcctgg aaaatggcgt ttccggtatc ttcaacctcg gcaccggtcg
     4441 tgcagaatcc ttccaggcag tagcagatgc tacgctggct tatcacaaga aaggccagat
     4501 cgaatacatt ccgttcccgg ataagctgaa aggccgctac caggcgttca ctcaggcaga
     4561 tctgacaaat ctgcgcgcgg cgggttacga caaaccgttc aaaaccgttg ctgaaggtgt
     4621 aacggaatac atggcctggc tgaatcgcga cgcataagag ctctgcatga aaatactggt
     4681 gatcggcccg tcttgggttg gcgacatgat gatgtcgcaa agtctctatc gcacgctcca
     4741 ggcgcgctat ccccaggcga taatcgatgt gatggcaccg gcatggtgcc gtccattatt
     4801 atcgcggatg ccggaagtta acgaagctat ccctatgcct ctcggtcacg gagcgctgga
     4861 aatcggcgaa cgccgcaaac tgggtcatag cctgcgtgaa aagcgctacg accgcgccta
     4921 cgtcttacca aactccttca aatctgcatt agtgcctttc ttcgcgggta ttcctcatcg
     4981 caccggctgg cgcggcgaga tgcgctacgg tttactcaac gatgtacgcg tgctcgataa
     5041 agaagcctgg ccgctaatgg tggaacgcta tgtcgcgctg gcctatgaca aaggcattat
     5101 gcgtaccgca caagatctgc cgcagccatt gttatggccg cagttgcagg tgagcgaagg
     5161 tgaaaaatca tatacctgta atcaattttc gctttcatca gaacgtccga tgattggctt
     5221 ttgcccgggt gcggagtttg gtccggcaaa acgctggcca cactaccact atgcggaact
     5281 ggcaaagcag ctgattgatg aaggttatca ggtggttctg tttggctctg cgaaagatca
     5341 tgaagcgggc aatgagattc ttgccgcttt gaataccgag cagcaggcat ggtgtcggaa
     5401 cctggcgggg gaaacacagc ttgatcaagc ggttatcctg attgcagcct gtaaagccat
     5461 tgtcactaac gattctggcc tgatgcacgt tgcggcggcg ctcaatcgtc cgctggttgc
     5521 cctgtatggt ccaagtagcc cggacttcac accgccgcta tcccataaag cgcgcgtgat
     5581 ccgtctgatt accggctatc acaaagtgcg taaaggtgac gctgcggagg gttatcacca
     5641 gagcttgatc gacattactc cccagcgcgt actggaagaa ctcaacgcgc tattgttaca
     5701 agaggaagcc tgacggatgc gggttttgat cgttaaaaca tcgtcgatgg gcgatgttct
     5761 ccatacgttg cccgcactca ctgatgccca gcaggcaatc ccagggatta agtttgactg
     5821 ggtggtggaa gaaggggtcg cacagattcc ttcctggcac gctgccgttg agcgagttat
     5881 tcctgtggca atacgtcgct ggcgtaaagc ctggttctcg gcccccataa aagctgaacg
     5941 caaagcgttt cgtgaagcgc tacaagcaga gaactatgac gcagttatcg acgctcaggg
     6001 gctggtaaaa agcgcggcac tggtgacgcg tctggcgcat ggcgtaaagc atggattgga
     6061 ctggcaaacc gctcgcgaac ctttagccag cctgttttac aattgtaagc atcatattgc
     6121 aaaacagcag cacgccgtag aacgcacccg cgaactgttt gccaaaagtt tgggctatag
     6181 caaaccgcaa acccagggcg attatgctat cgcacagcat tttctgacga acctgcctac
     6241 agatgctggc gaatatgccg tatttcttca tgcgacgacc cgtgatgata aacactggcc
     6301 ggaagaacac tggcgagaat tgattggttt actggctgat tcaggaatac ggattaaact
     6361 tccgtggggc gcgccgcatg aggaagaacg ggcgaaacga ctggcggaag gatttgctta
     6421 tgttgaagta ttgccgaaga tgagtctgga aggcgttgcc cgcgtgctgg ccggggctaa
     6481 atttgtagtg tcggtggata cggggttaag ccatttaacg gcggcactgg atagacccaa
     6541 tatcacggtt tatggaccaa ccgatccgag attaattggt gggtatggga agaatcaagt
     6601 agagtgtcgt tctacaagta tgtctcttgc agatttgcca gctcaaaccg tttttcaaaa
     6661 tttaaatctt gaaataataa ccaataagtt gacatcggag ataagatgac ctcaacatta
     6721 tttttctctc tcgagaaaaa aaactggata gcgtactgga acagagctct cgtattctta
     6781 ttcattacca cctatttttt gggtgggatt acaaggtata aacatcttat tgttattctt
     6841 atgacaataa cgacaatcgt ctatctctgc aaacggccaa aacactatct ctcactattt
     6901 aaaacatttc tttttggtag tgttgccata ttaactattg ctgcattgct gtcacttctt
     6961 caatcccctg atgcaggtgc tagcatgaag gaaattttca aagctattat tgagaatact
     7021 ttactatgca caatagcaat accggtcata ttgagagacg agaaaagaga agatgtcgaa
     7081 aaaatcgttt tcttctcatt tattagtgcg ttgggcttac gctgtttttc tgaattgatt
     7141 acctattata aggactatca acaagggata atgccattcg cagattatag acaccgtagc
     7201 atttctgact cgatggtctt tttattccct gcattgttaa atctctggct tatcaaatca
     7261 gcaaaatacc gcatttcttt tgtggttcta agcgttattt ttatttttct gatattagga
     7321 actttatcca gaggggcctg gctttccgtg ttagtcattg gattaatatg gattctgatg
     7381 tttaaacaat ggaagttact attagtagga gtaatggttt ccatcattgc attgtcggtt
     7441 attttcacac ataaggagat gaccgcaaag ctaacgtata aacttcaaca aactaatagt
     7501 tcttatcgct atgcaaatgg tactcaaggc agcgcactcg atctaatatt agaaaatcct
     7561 gttattggtt atggttacgg taacgttgca tataaagatg tctataataa acgtgtcatt
     7621 gattatccag aatggacctt tagacaatca atagggccac ataattttgc gctattcatc
     7681 tggtttggca ctggtttatt agggctggta agtcttatga tgctatactg tgcaatattg
     7741 aaagagtgta taaaaaatgg cgtcaagaat aaatatcgct caccatataa tgcatattat
     7801 ataatcttac tatcttttat aggttatttt gttatccgtg gaaacgtaga acaaattgaa
     7861 ccaaatttat taggcgttta cgccggctta ttattagcga tgaaaaacaa gtaaaaaata
     7921 aaaaaggctg cataatgcgg cctttttatt tattcaaacc aattatgaat aacctcttcg
     7981 aatctttgag ttacgccatc ccaactgtag tgatcaaaca caaagtcctg accttgcttt
     8041 gcaactgctg ttaattcagg attcgctaac gtttttaaaa tatcgctgct tatggagtct
     8101 gcagtcatag gttcttttaa atggaatccc gtagtatttt ctttgacaaa ttccgtcatc
     8161 cccccacgcg tactcactaa aacaggctta ccagcaccca tagcttcaat tgccaccatg
     8221 caaaatggtt cctggaactg ggaaggaatg acgactaaat ctgccagtgg gtaataacaa
     8281 tgcatctttt caggcggtat gcttcctaac ataatgcaac gatccttcaa acgttttgca
     8341 atttccctga cattgcgttg atatgcacct ttgtcacttt tactcatttc tgtataatca
     8401 ccaatcacaa ctaattttaa atttttgtga gcagcggcca atttttcgaa tgattgcatc
     8461 aacagcaata tgcctttgtc agggacgata cgcccagcat aaaaaataat tttctcttca
     8521 ggggagatat taagttcgct tctagtgatc ggctgaaaat tagattgata ggtttctaaa
     8581 tcaattccat ttggtacaat ctcaatatct gcatttgcta aataagattg gtaatacttt
     8641 ttcaagtata aactagggac tatcatttta acattttgct caagcaattt tggttcaaat
     8701 gcattatgca tatgtataac cactcttgcc tgaggagctc gcttacgaat ttgagtgtat
     8761 agcttcatgc tattatgaac tacaataaca ctttctttcg ttataggaaa atcatgtgca
     8821 atattcaata ttctctgtga atatggaaga ggatcaagtc ttgtccattt ttgaaataac
     8881 cgcttatata tgcgactgaa tccaacccta tgaatactac aattatcatt aacaaaggtg
     8941 taatttgagt atcctggatt tttgatacaa acaatacgat ttggaaaatt ggtacgctga
     9001 gcaacctgat acatccacgt ttcaacagca gccgctccgc gaggaggaat tgaaaaaata
     9061 ggagtaaccg taaatattat tttatcaacc atctcgtata ttaacctttc ataacattat
     9121 atttaattgc tgtaaaaaca ccatcgagaa attttttctg gtaaagcaag tgtttatatt
     9181 tttctttgtg ctcatgtttt ttaacagctt ttttataagg tatatttctc catggtgata
     9241 tattataaat attacgaaaa taatctgcag aggcgtagtt cgcccaatca tgccatggct
     9301 tagttatacc agtataatgt atgaagacag tgtcatcatt aataaaccgg gtgtaatatt
     9361 cactattttt ttcttcaaat tctgacttta tagtataaat acaattatat tttctcggta
     9421 atattttagc atgatgtaga aacattaagt ttaaaatatc ttgatccggg tactttaatt
     9481 tttttataat tgcctcatct gataatagat caaagaattt ttccgttagt ctttgtttta
     9541 accattcccg taaattaata tacattacac cagagttaaa atagctaccg ttaaaatcct
     9601 cattacacaa acgctcagcg cttttacttt gcatagcatc tacatcaatt accactgccc
     9661 catattcatc tttaaactct aatgctatta actcgttcaa tgaaccctta cagacgatat
     9721 cagcatcaag atataataat gaatccaatc gcgcagaaaa ataatcaaat gaaagcaaac
     9781 gataataaat agatactggc cagatatttg attgaggtag ctccttcaat ggctcagaat
     9841 caatatgata taatttaatt actgtttgat acttcgttgc taattgtgat aataattcaa
     9901 tatactcttt gtctaagtaa tcagcgatta tgtgaaaggt aaatttgttt tgtttattat
     9961 tttcaagaat tgaagcaatt gaaatcgcag ccccaacctg gtagttctca tcaatacccc
    10021 atgagacatt aaacgtttcc cgttcatcaa gtttaacggg gcgcttgtcc agttcaatta
    10081 tatctttaaa ttgagtaaga tgtttaaaat ccaatttaat tacccttcat cataatcatt
    10141 atttgctctt caatcctagt ttgactttta catccctgat tactttttta attttctttt
    10201 tgaatataag atatgtatag ccaaagtctt tcaattcatt ttttatatta tagtgccgtt
    10261 caagatcaat gcgatctttt gcttttaaaa cagcggttgt ttttttacct gaaaggtcaa
    10321 ttagtcttaa acctttttcg gatactataa aatttccttt atgaggatcg ccagacacca
    10381 ttccatgctg atgcaactct tttatcgatt ctgataattg atccttcaga tcttcagaaa
    10441 tttccaaata ctcatttaac ccgacacctt caatatactc aatgagcata atataataat
    10501 gagcgaaatt taatgtttta cgttcagcta gcaaaaaata gtcattaatt gattggatgc
    10561 cttcaccacg tacccgatcg gtttgataaa ttaaattttc ataataatct ttttttatgc
    10621 aagattttaa gaaacgttcg gtcattttat gctttgccgc ataaacttta agtacaagag
    10681 gtccacgagc agtgtcaata agaatgactt tagtatcatc aatagaacga aaaactttta
    10741 atgttttaac cctgcaagtt aaaaagtcat ttagaacttg ctcataaaag ggatcattat
    10801 cttttatgaa aactttcaac ccatttatcg ttttattata tatcataatg aattatttta
    10861 accttaaatc tttagaagca tttttcttta taatacttta aataattaaa aatgcctgcc
    10921 atataatgct tttgtttaaa tttatgtttt gcacaatacc gatactgatt gctatttaca
    10981 ggtttaagta aatcttcttt actccacgga gaagctgctt tggcgatcaa aaaactacgt
    11041 gaaactggat aatcagccca ctcatgccag ggttttgtag gtcccacata gtgtataaac
    11101 acggtgtcat cattgactgg attatcaact ttgtctttta attcatagtt aatactatat
    11161 cgggtattat atttctccga aataaacttc actttaccat tcaataatac attcagtaca
    11221 tcttgatcaa ggtgggtgat tttacttacc caatctgggt cacgcaacat ttcaatagct
    11281 tttgacgaaa tgttatttag attccactca tcaatattta tcagtaaaaa accagcattg
    11341 aaatatccag aagctaactg tggtgtagtt aataccgagg ctcgattttg ccaccattca
    11401 atatctcttt cagctacaac tgcggcaatt tcattagtag aaaattgata atctaagagt
    11461 tctttaatac tacccttgca agcaatatct gcatcaagat atagtatttt ttcatgttta
    11521 tgatagaaat aatctgcaat tataaatcga aaatatgttg cgtaagtcca gtttttcgtg
    11581 cttggtaatg acttcagctt atcacaattg ataacataaa tattaattct taaattatat
    11641 tgttttgcta aatcagaaaa atataatttg tctttatcac tgatataatc tgtgaaaaca
    11701 tgaaattcac aagagatttc tctattgttt aatagaatag atgcgatggc tacaccacat
    11761 ccaaaaagaa aatttctgtc aataccaaat gctatatcaa gagtattttt agatcttata
    11821 ttttgataat gaaaattata ctcaaaaagg atgatgtcac tatcattgag ttgagacata
    11881 tttattctgt atccaaaatt ttggcaaaaa taagttacgg gttacaacga ttttcgaatc
    11941 gttctttccc tgatttttgt ggcttttgct tctgcttgcg acagcagtcc ttgttcctgc
    12001 ttgagaatgt ctttaagcgg ggcggcaaaa tacaccttca taaagcgcca gatatcccgc
    12061 tgagtcaggc caatattcat tgaagaaaaa taaagcccta taagatcttt atcgcgccaa
    12121 cgacgtggaa cgcgcgtgcg aagctgcgcc cggtgtaggt caattaccga aatttttaac
    12181 tcctcttcct taccggaaaa aggcaagtgc agcaggaaat gacagatata acagtcacga
    12241 tggttaatgc ccgcagcatg catatcgcgc accatcgtcg cgacacgctt aataagcata
    12301 cgctttacgc gaacatctgg tgggttggtc gcccagtcag cacagtaatc ttccagactg
    12361 atggttggtg tcagatcttc ggtaataata aacgaggttc tggtcagcgg attcatgcct
    12421 ttttcgccga atgccacccc atacatagta tcaacgccga catcccgcag tcgatgaatc
    12481 gcattccatt cgcggtctgc gcctaatact ggcatccgca atgagagtaa atttttgatt
    12541 atctctttca gggtcgtgcc gcgatgccat ttgagaaaat agctttttcc cgccatttca
    12601 aagcgcagag tacggcgagt ttccagttca cgaaatacct caccctgcaa ggttttaact
    12661 tcctcaaaag gatctttgcc gcgccataac gtggcaaacg gctctttaag ttcaaccatc
    12721 cagaccaccc gttatgatat ccgccgcttt ctctggcaga ctgtataaat cttgtgtatc
    12781 agcataatgt cgcgcatttt ccgcccaagc ctggcgcaat gaagattgcg ttaacgcttt
    12841 gcgtaaaatc tcattcaatg tttcctggcg gaatggctca gcaatagcct cgccgcaatt
    12901 agcgtcgaca atataatgcg catagccaca aacggcagtt gttagtaccg gtaatcctgc
    12961 agtaatcgct tccagcagca caattcccgc cgcttcctgg taggcaggat gcagtaataa
    13021 atccgccgcc gccattaatt ccgagacatc gttgcgcccc gagaagaagt gaacattact
    13081 gcgcacgccg cgtttttctg ccagtgcctc aaattttcgc ggtttatcct gcccaacaac
    13141 atatagcaat gtgttgtggc gcagcgaatc cggtaacgaa gcaagtgctt caatggaacg
    13201 atcgacacct ttacgcgtga agtctgaacc gacctgcaac aataaatatt gttgttcggt
    13261 tattccattc ttcttacgga agatttcacg gctattggct ggctgctggc tatatttacg
    13321 atcaggataa atccccggtg gcagaatatg aaaacgctcc gcttcagtct gataatgttt
    13381 ctggaaatcg gcgatttgct tatctgtcag catcagcagc tgtgtcggct tgctctgttc
    13441 gaaggttgcc cgctcaaagg cggcataatg gcgataacgt gacgtcaggc gatagaaaaa
    13501 gcctttttcc tgcgctactt tctcggcata acaaacatca gcggcataat aaacgtccag
    13561 ccccggcatt ttgttgaatc caacgacttt atcgacggga tgttcgcgta aatgtttttg
    13621 cacccaggca aaatactccg cattgcgccc gtgattggta tgcgatttaa ccggcacttt
    13681 gatcagttca aatacatcag ggcattcgcc ttcccacgac tgggtataaa cccgaacatg
    13741 atgacctcgg gcggcgactg tctgagcaat acgcataaaa tcgcgctgca aaccgccaaa
    13801 gggaaaatat ttatataaac aaaaagcaac gatcataacg acgtacctgt cgtggaggag
    13861 ggcagtaatt tatcgacagc agcaatgaca tctgccgccg gaataaccga aagatacatc
    13921 tcatttcggt cacgctgatc gcgcgttggc atttcccggt aatctcccgc ccagaattga
    13981 atcatgttat ttgaccaggg acgccagaaa atatgatctg ttgcgccaaa cagcgatatc
    14041 agcggcgtat taactgcagc ggcaatatgc gccggtgcgg aatcaacgcc aataaacagc
    14101 tgcgcatgat cgattaacgc accaagttcc gggaaggtca cctttccagc cagcgccgtt
    14161 actggtggcg tctggcatcc ctgcgcaatt tcattgacgc aggccagatc gtctttatct
    14221 gggccggacg tcagaacgac ttcataacca cgagcatgta aggcatcaat cacagcggaa
    14281 aacttggcgt tgtcccagca tttgaagatt tgccgcgccg taggttggat aaccacataa
    14341 ttttgtccaa caccagcgtg atcaagttcg cgacgcatac gtttccagct tgcaggcggg
    14401 taactcatgg ttgtctgctt caccaacgaa tcaagtccca atggtgtcag cacggataag
    14461 ttactttcca ccacatttcc atcctgcaac ggcaccaaat gggtgaaact tttacgccaa
    14521 aaagcagact gccgatgatg ataatcctgg gaaattttca cacgagcatt taataagcga
    14581 accagtatag caaccatcca ttgatcggta agattgacga taaggtcata cttattggca
    14641 cgtaatacct tgatgagatg aaaaaagttg gcaatttttt ctgaggcttt tgctttttta
    14701 ttttttatgc cgtagagcgc gttaatctct ggattttcag acaggatcgg gatggtgtcc
    14761 tgataaagca gcacatcgat ttttgcgtca aggtaatttt ttttcagcga actaatgacg
    14821 ggagtagtta ataacatatc cccatgaaaa cgcatcttaa tgagcaaaat tcttcgaaat
    14881 ggcttatcca caattaactc tttttgttga gattatccga ttaaggtgaa gcagaaaaag
    14941 cacgctaccg ccccaggctc aacagctacc tgaatactga taccgcactg atattgacgc
    15001 gctagtgtat cacttctttc gcggcaatcc gacccgaatg atttttaagc acattgttga
    15061 ttttcaataa agaaatcaat caaataaaat cattagcaac catattcttt cactgttgct
    15121 ctgcgattcc ttcctgcacg tacagcagaa atcaatgaat cgcataaaaa tacaaaaaaa
    15181 gtatagatgt tcagcaagaa ttggcgggta aattgctatt taagtcaaaa acgggaaaag
    15241 taatggtaaa gccacagcta aatacataga atccccagca catccataag tcagctattt
    15301 actatgctcg aattgcttta caccgccctt ctctacctta ttcagccgct gatctggata
    15361 cggctctggg tgcgcggacg taaggctccg gcctatcgaa aacgctgggg tgaacgttac
    15421 ggtttttacc gccatccgct aaaaccaggc ggcattatgc tgcactccgt ctccgtcggt
    15481 gaaactctgg cggcgatccc gttggtgcgc gcgctgcgtc atcgttatcc tgatttaccg
    15541 attaccgtta caaccatgac gccaaccggt tcggagcgcg tacaatcggc tttcgggaag
    15601 gatgttcagc acgtttatct gccgtatgac ctgcccgatg cgctcaaccg tttcctgaat
    15661 aaagtcgacc ccaaactggt gttgattatg gaaaccgaac tatggcctaa cctgattgcg
    15721 gcgctacata aacgtaaaat tccgctggtg atcgctaacg cgcgactctc tgcccgctcg
    15781 gccgcaggtt atgccaaact gggtaaattc gtccgccgct tgctgcgtcg tattacgctg
    15841 attgctgcgc aaaatgaaga agatggtgca cgttttgtgg cgctgggcgc aaaaaataac
    15901 caggtaaccg ttaccggtag cctgaaattc gatatttctg taactccgca gttggctgct
    15961 aaagccgtga cgctacgccg ccaatgggca ccacaccgcc cggtatggat tgccaccagc
    16021 actcacgaag gtgaagagag cgtggtcatt gccgcacatc aggcattgtt acagcaattc
    16081 ccgaatttat tgctcatcct ggtaccccgt catccagaac gcttcccgga tgcgattaac
    16141 cttgtccgcc aggctggact aagctatatc acacgctctt caggggaagt cccctccacc
    16201 agcacgcagg ttgtggttgg cgatacgatg ggcgagttga tgttactata cggcattgcc
    16261 gatctcgcct ttgttggcgg ttcactggtt gaacgtggtg ggcataatcc gctggaagct
    16321 gccgcacacg ctattccggt attgatgggg ccgcatactt ttaactttaa agacatttgc
    16381 gcgcggctgg agcaggcaag cgggcttatt accgttaccg atgccactac gcttgcaaaa
    16441 gaggtttcct ctttactcac cgacgccgtt taccgtagtt tctatggccg tcatgccgtt
    16501 gaagtactgt atcaaaacca gggcgcgcta cagcgtctgc ttcaactgct ggaaccttac
    16561 ctgccaccga aaacgcattg aggttgttat gcaaaaacgg gcgatttatc cgggtacttt
    16621 cgatcccatt accaatggtc atatcgatat cgtgacgcgc gccacgcaga tgttcgatca
    16681 cgttattctg gcgattgccg ccagccccag taaaaaaccg atgtttaccc tggaagagcg
    16741 tgtggcactg gcacagcagg caaccgcgca tctggggaac gtggaagtgg tcgggtttag
    16801 tgatttgatg gcgaacttcg cccgtaatca acacgctacg gtgctgattc gtggcctgcg
    16861 tgcggtggcg gattttgaat atgaaatgca gctggcgcat atgaatcgcc acttaatgcc
    16921 ggaactggaa agtgtgtttc tgatgccgtc gaaagagtgg tcgtttatct cttcatcgtt
    16981 ggtgaaagag gtggcgcgcc atcagggcga tgtcacccat ttcctgccgg agaatgtcca
    17041 tcaggcgctg atggcaaaat tagcgtagcg tttatgccgg atggtatgcc atccggctcg
    17101 catgaattac ttctggcact gccgacaata aaacgttgcc cgctgcgcat gtttagtcgc
    17161 cacaatcggc gtaccgcaca cccgacacgg ctcacctttt cgcccgtaaa cctgcaattc
    17221 ctgcgcgaaa tagcccggtt taccatcact ttgcagaaaa tctttcagcg ttgtaccacc
    17281 ctgctcaatc gaacgcagca acaccgcttt aatcacccga gctaacaatt cacactctgc
    17341 cagcaacagt gatgacgcca gccgatccgg atggatcccc gccgcaaaca gtgattcgct
    17401 ggcatagata ttccctaccc ctaccaccag cttgttatcc atcagccacg gtttaatcgc
    17461 cgtttttttc ttcgcgcact tctgatgcag atactcacca ttgaaatcgt cgctaagcgg
    17521 ctccggtcca agatgggcca gcacattatg cccttccagc tctttggtcc acagccaggc
    17581 accaaagcgg cgcggatcgg tgtagcgcag cactttaccg ttgctcatca ccaaatccac
    17641 atggtcatgc ttttcagggg gaagttcttc tggaaggatg cgcaggctgc cagacatccc
    17701 caaatgaatg ataatccagc cctcaggcag ctccagcagc agatatttag cccgccgctg
    17761 cacgctaagc actggttgat cgcttaatcg gtagatctct tctgaaaccg gccagcgcaa
    17821 gcgtccgttg cgcaccaccg catgaagaat ggttgcacca acgagatgcg gttctatgcc
    17881 gcgacggctg gtttcaactt cgggtaattc aggcatagca tctccaggaa tgaacagatg
    17941 caatcaatat gggggcaagc agataacaaa aaaccccgcc ggagcgaggt ttttcgttac
    18001 atcaaagcga gaattatttg attttcgctt ctttgtagat cacgtgctga cgaacaactg
    18061 gatcgaattt tttcagttcc agtttttccg gcttagtacg tttgttcttc gtagtggtat
    18121 agaagtgacc agtaccagca gaagaaacca gcttgatttt ctcacgaata cctttagcca
    18181 tgatttattt cctctaagta cttagtactt ttcgccacgg gcacgcagtt cagccagaac
    18241 tgtatcgatg ccttttttat cgattacacg cataccttta gcagatacgc gcagggtgac
    18301 aaaacgcttc tcgctctcaa cccagaaacg gtgagagtgc aggttcggca ggaaacggcg
    18361 tttagtcgcg ttcagtgcgt gggaacggtt gttaccggtc accggacgct tgccagtaac
    18421 ttggcagact cgggacatgt ctattctcca aaaatcaaat tagctcgagc ttcgtatggg
    18481 gtattggcgc ctcgtcaggc tttacagccc ggtcatcgca gttctatgtg aactctcgat
    18541 tgccaggccc aaatgccaaa cccgagattc tcaaaggtgg cgtagtatac gctgactcag
    18601 cgatgtgctc aagtcccgaa cagacaaaga tcccgaagga tcgcgcatag cgggttaaat
    18661 ccagccgcgt tcggcaaaag aaacatactc tccacgccca atcacgatat ggtcgagcac
    18721 gcgtaaatcc atgaactgac aactctttat tatccgttca gtaatgagtt tatccgcttt
    18781 actgggttca gcacaacccg aagggtgatt atgtgcaagg atcagcgccg aggcgtttat
    18841 ttttatcgct tcgcggataa tttcccgagg atggacttca acatggttta gcgtgccgga
    18901 aaaaagacgg ctatgcgtta taacccggtg ttgtgagtcg agaaagatca ccataaagat
    18961 ctcccgctcc tcacccgtga gctggctttg taaaaattcc cgcgtcatct ccgggctgag
    19021 taaagggctt tcttcacgca tccgcacgtt gtagtaacgt cgcgccagtt cagcaatccc
    19081 ctttaactgg gcaaatttcg ctacgccaat tccatgaaca ccactaaatt gttcatattc
    19141 agaggttaac aagccataaa gagagccgaa attctccagc atctcttttg ccagggttaa
    19201 tacatcttta ccgcgcgttc cggtacgcag aaatagcgcc agcagctcga catccgttaa
    19261 ggcgctaata ccaaacttca gcattttttc gcgcggcatc aacagctgtg cattgttttt
    19321 caccttcacc tcctttgtgg tgctcgcatc ctgtcatagc cgttccgtta aatcgacggc
    19381 cagttttcaa tcctggaaag agcctcgcaa agtgaatctc aggtgaaggc acgacaacaa
    19441 aagtattgtg ataaaatcgc caacttctgg tgtcacccaa caggaaaaat catcatgagc
    19501 ctggccggta aaaaaatcgt tctcggcgtt agcggcggta ttgctgccta taaaacccct
    19561 gaactggtgc gtcgtttgcg cgatcgcggg gccgacgtcc gcgtagccat gaccgaagcg
    19621 gcaaaagcct ttatcacccc acttagcttg caggcggttt ctggttatcc cgtttccgac
    19681 agtctgcttg acccggcagc cgaagccgct atgggccata ttgagctggg taaatgggct
    19741 gatttagtga ttctcgcccc tgccacggca gatttgattg cccgtgttgc tgccggaatg
    19801 gcgaatgacc tggtatcgac gatttgtctg gctacacctg cgcctgtagc cgtgctcccc
    19861 gccatgaacc agcagatgta ccgtgccgct gccacgcagc ataatttaga ggtgcttgct
    19921 tcccgtggtt tgctcatctg ggggccagac agtggcagtc aggcttgtgg tgatatcggt
    19981 cctgggcgaa tgctcgatcc gttaaccatt gtggatatgg cggtggcgca tttttcgccc
    20041 gtcaacgacc tgaaacatct gaacattatg attaccgccg gtccgacgcg tgaaccgctc
    20101 gatccggtgc gttatatctc taatcacagc tccggcaaga tgggttttgc tatcgccgcc
    20161 gctgctgccc gtcgtggcgc gaacgtcacg ctggtatcag gtccagtttc actaccgacg
    20221 ccaccgtttg ttaaacgtgt tgatgtgatg accgcgctgg aaatggaagc cgccgtgaat
    20281 gcttctgtac agcagcaaaa tatttttatc ggttgcgccg ccgtggcgga ttatcgcgca
    20341 gctaccgtgg ccccagaaaa aatcaaaaag caggccacgc agggtgatga attaacaata
    20401 aaaatggtta aaaaccccga tatcgtcgca ggcgttgccg cactaaaaga ccatcgaccc
    20461 tacgtcgttg gatttgccgc cgaaacaaat aatgtggaag aatacgcccg gcaaaaacgt
    20521 atccgtaaaa accttgatct gatctgcgcg aacgatgttt cccagccaac tcaaggattt
    20581 aacagcgaca acaacgcatt acaccttttc tggcaggacg gagataaagt cttaccgctt
    20641 gagcgcaaag agctccttgg ccaattatta ctcgacgaga tcgtgacccg ttatgatgaa
    20701 aaaaatcgac gttaagattc tggacccgcg cgttgggaag gaatttccgc tcccgactta
    20761 tgccacctct ggctctgccg gacttgacct gcgtgcctgt ctcgacgacg ccgtagaact
    20821 ggctcagggt gacactacgc tggttccgac cgggctggcg attcatattg ccgatccttc
    20881 actggcggca atgatgctgc cgcgctccgg attgggacat aagcacggta tcgtgcttgg
    20941 taacctggta ggattgatcg attccgacta tcagggccag ttgatgattt ccgtgtggaa
    21001 ccgtggtcag gacagcttca ccattcaacc tggcgaacgc atcgcccaga tgatttttgt
    21061 tccggtagta caggctgaat ttaatctggt ggaagatttc gacgccaccg accgcggtga
    21121 aggcggcttt ggtcactctg gtcgtcagta acacatacgc atccgaataa cgtcataaca
    21181 tagccgcaaa catttcgttt gcggtcatag cgtgggtgcc gcctggcaag tgcttatttt
    21241 caggggtatt ttgtaacatg gcagaaaaac aaactgcgaa aaggaaccgt cgcgaggaaa
    21301 tacttcagtc tctggcgctg atgctggaat ccagcgatgg aagccaacgt atcacgacgg
    21361 caaaactggc cgcctctgtc ggcgtttccg aagcggcact gtatcgccac ttccccagta
    21421 agacccgcat gttcgatagc ctgattgagt ttatcgaaga tagcctgatt actcgcatca
    21481 acctgattct gaaagatgag aaagacacca cagcgcgcct gcgtctgatt gtgttgctgc
    21541 ttctcggttt tggtgagcgt aatcctggcc tgacccgcat cctcactggt catgcgctaa
    21601 tgtttgaaca ggatcgcctg caagggcgca tcaaccagct gttcgagcgt attgaagcgc
    21661 agctgcgcca ggtattgcgt gaaaagagaa tgcgtgaggg tgaaggttac accaccgatg
    21721 aaaccctgct ggcaagccag atcctggcct tctgtgaagg tatgctgtca cgttttgtcc
    21781 gcagcgaatt taaataccgc ccgacggatg attttgacgc ccgctggccg ctaattgccg
    21841 cgcagttgca gtaatatgac gccggatgac ttttcatccg gcgagtttct ttaaacgcca
    21901 aactcttcgc gataggcctt aaccgccgcc agatgttccg ccatttccgg cttctcttcc
    21961 agataggcaa tcagatcttt cagggtgatg atagagatca ctttgcagtt gtaatcacgc
    22021 tcaacttcct gaatcgccga aatctcgccg cgcccgcgtt cctgacgatc gagcgaaatc
    22081 aacacgccag caagcgtcgc gccattggcc tgaataatct ccatcgactc gcgaatcgcc
    22141 gttccggcgg tgatcacatc atctaccagc attacgcgtc cttgtaacgc gctaccaacc
    22201 agattgccgc cttcaccgtg gtcttttgct tctttgcggt taaagcagta cggcaggtcc
    22261 aagtcgtgat gctccgccag tgccacagcg gttgtggtgg caatcgggat ccctttgtaa
    22321 gcagggccaa acagcagatc gaactcaatg ccggaatcca ccaacgcttc agcgtaaaaa
    22381 cggcctaaca gtgccagatc gcgcccggta ttaaacagcc cggcgttgaa gaaatagggg
    22441 cttttgcgcc cggatttcag cgtaaactcg ccaaacttta acacctgctt gctaagcgca
    22501 aattcaataa actggcgctg atatggtttc atgccttcgc tcctcatctt acttttctac
    22561 agacaaaaaa aaggcgactc atcagtcgcc ttaaaaatca gtttgccagc gccgccttct
    22621 gcgtcgctac aatggattcg attccccctc gggccagcgc caacaaggtg agtagctctt
    22681 catgggtgaa cggctcgcct tctgccgtcc cctgcacttc aatgatgcgc ccgtcttcgg
    22741 tcatcactac gttcatgtcg gtctctgcgg ctgagtcttc aacgtattcc agatcgcaaa
    22801 ttgcttcacc gtttacaata ccgacagaaa ctgcggctac catccctttc atcgggttgg
    22861 ttttcagctt gccgttttcc accagcttct gtagcgcatc tgccagcgcc acgcaggcac
    22921 ccgtaatcga cgcggtacga gtgccaccat cagcctgaag cacgtcgcag tccagcgtaa
    22981 tggtgaactc acccagcgct ttcaaatcta ctgccgcgcg aagagcacgg gcgatcagac
    23041 gctggatttc cattgtgcgt ccaccctgct tacctttcgc cgcttcacga gcgttacggg
    23101 tgtgggtaga acgtggcagc atgccgtact ctgcggtgat ccagccctgg ccctgacctt
    23161 tcaggaagcg cggcacgcct tcttcaatag aggcggtaca caatactttg gtatcgccaa
    23221 attcgaccag caccgagcct tctgcatgtt ttgtatagtt acgagtcagg gtaacgggac
    23281 gcacctgatt attgctacgg cctgctggac gcatattgaa atctccggct tgaaacaaat
    23341 gtggctgcgc attatacgga cttccggcgg ttattcctat cctgacaagg catcgatggc
    23401 tataatcctt ccacctctcc ttttataaac aggaacgtct atgatccgca gtatgaccgc
    23461 ctacgcccgg cgtgaaatca agggtgaatg ggggagcgca acctgggaaa tgcgctcggt
    23521 aaaccagcgt tatctggaaa cttactttcg tctgccggag cagttccgta gccttgaacc
    23581 tgtcgttcgc gagcgtattc gttctcgcct gacgcgcggt aaagtggaat gtaccctgcg
    23641 ctatgagcca gatgttagcg cacaaggtga gctgatcctc aatgaaaaac tggctaaaca
    23701 gctggtaact gccgcgaact gggtaaaaat gcagagtgac gaaggggaaa tcaacccggt
    23761 tgatattcta cgctggccgg gcgtgatggc agcccaggag caggatctcg acgcgattgc
    23821 cgctgaaatt ctcgcggcgt tggatggtac gctggacgac tttatcgtcg cgcgcgaaac
    23881 cgaaggtcag gcactgaaag cattgatcga gcagcgtctg gaaggcgtca ccgccgaagt
    23941 ggtcaaagtc cgcgcccata tgccggaaat tatgcaatgg cagcgtgagc gtctggtcgc
    24001 gaagctggaa gatgctcagg ttcagttgga aaacaaccgt ctggagcagg agttggttct
    24061 gctggcacaa cgaattgacg ttgccgaaga gctggaccgc ctcgaagcgc atgttaaaga
    24121 gacgtacaac attctgaaga aaaaagaagc ggttggtcgt cgtctggact ttatgatgca
    24181 ggagttcaac cgtgagtcga acactcttgc gtcgaaatct atcaatgccg aagtgacaaa
    24241 ctccgccatc gagctgaaag tgttgattga gcagatgcgc gagcagattc agaatattga
    24301 gtaactcgta acaccaattc tgacttttca gccttcacaa atgccgccca ctcaaacaga
    24361 gcggcatttt tcttccccgc aacttcaatt ctgttttgcg tgcctgctcc agattttgcg
    24421 atgttttttt gcccagcaca ctgagaacgt gagatactca caactgtata taaatacagt
    24481 tacagattta ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc
    24541 aaccttttga agagataaaa ctgattaatg caaactgaac cgccccggg
//
LOCUS       DADLSB010000048        24321 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.048, whole genome shotgun sequence.
ACCESSION   DADLSB010000048 DADLSB010000000
VERSION     DADLSB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.048"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1582
                     /locus_tag="JN331_001890"
     CDS             <1..1582
                     /locus_tag="JN331_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7260051.1"
                     /translation="LAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAA
                     GRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGAISVD
                     ATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNK
                     DNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSS
                     KDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQIYMS
                     NSTTFDDQGFASNNTGLSGPVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVN
                     GSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTT
                     NRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKP
                     WFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLS
                     CTITFGKEIDESRNYICQ"
     gene            1598..2631
                     /locus_tag="JN331_001891"
                     /pseudo
     CDS             1598..2631
                     /locus_tag="JN331_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(2713..3051)
                     /gene="rcnB"
                     /locus_tag="JN331_001892"
     CDS             complement(2713..3051)
                     /gene="rcnB"
                     /locus_tag="JN331_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY7260052.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3270..4118)
                     /locus_tag="JN331_001893"
     CDS             complement(3270..4118)
                     /locus_tag="JN331_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAY7260053.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HARDHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLFTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            4240..4512
                     /gene="rcnR"
                     /locus_tag="JN331_001894"
     CDS             4240..4512
                     /gene="rcnR"
                     /locus_tag="JN331_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY7260054.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      4604..4700
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4735..5524
                     /gene="thiM"
                     /locus_tag="JN331_001895"
                     /pseudo
     CDS             4735..5524
                     /gene="thiM"
                     /locus_tag="JN331_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
     gene            5521..6321
                     /gene="thiD"
                     /locus_tag="JN331_001896"
     CDS             5521..6321
                     /gene="thiD"
                     /locus_tag="JN331_001896"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY7260055.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6386..7204
                     /locus_tag="JN331_001897"
     CDS             6386..7204
                     /locus_tag="JN331_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY7260056.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            7256..8002
                     /locus_tag="JN331_001898"
     CDS             7256..8002
                     /locus_tag="JN331_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7260057.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(7976..8941)
                     /locus_tag="JN331_001899"
     CDS             complement(7976..8941)
                     /locus_tag="JN331_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7260058.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(8938..9941)
                     /locus_tag="JN331_001900"
                     /pseudo
     CDS             complement(8938..9941)
                     /locus_tag="JN331_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(9938..11215)
                     /locus_tag="JN331_001901"
     CDS             complement(9938..11215)
                     /locus_tag="JN331_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7260059.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPNTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYTYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11472..12524
                     /gene="fbaB"
                     /locus_tag="JN331_001902"
     CDS             11472..12524
                     /gene="fbaB"
                     /locus_tag="JN331_001902"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY7260060.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            12832..13679
                     /gene="gatY"
                     /locus_tag="JN331_001903"
                     /pseudo
     CDS             12832..13679
                     /gene="gatY"
                     /locus_tag="JN331_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
     gene            13708..14970
                     /gene="gatZ"
                     /locus_tag="JN331_001904"
                     /pseudo
     CDS             13708..14970
                     /gene="gatZ"
                     /locus_tag="JN331_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            14980..15432
                     /gene="gatA"
                     /locus_tag="JN331_001905"
     CDS             14980..15432
                     /gene="gatA"
                     /locus_tag="JN331_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY7260061.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15463..15747
                     /gene="gatB"
                     /locus_tag="JN331_001906"
     CDS             15463..15747
                     /gene="gatB"
                     /locus_tag="JN331_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY7260062.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            15751..17106
                     /locus_tag="JN331_001907"
     CDS             15751..17106
                     /locus_tag="JN331_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAY7260063.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            17172..18212
                     /gene="gatD"
                     /locus_tag="JN331_001908"
     CDS             17172..18212
                     /gene="gatD"
                     /locus_tag="JN331_001908"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7260064.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18312..19091
                     /locus_tag="JN331_001909"
     CDS             18312..19091
                     /locus_tag="JN331_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7260065.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19173..20072)
                     /gene="yegS"
                     /locus_tag="JN331_001910"
     CDS             complement(19173..20072)
                     /gene="yegS"
                     /locus_tag="JN331_001910"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY7260066.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMPTGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            20478..20795
                     /locus_tag="JN331_001911"
     CDS             20478..20795
                     /locus_tag="JN331_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260067.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(21137..22498)
                     /locus_tag="JN331_001912"
     CDS             complement(21137..22498)
                     /locus_tag="JN331_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY7260068.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(22645..22977)
                     /locus_tag="JN331_001913"
     CDS             complement(22645..22977)
                     /locus_tag="JN331_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY7260069.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(23168..23890)
                     /gene="baeR"
                     /locus_tag="JN331_001914"
     CDS             complement(23168..23890)
                     /gene="baeR"
                     /locus_tag="JN331_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY7260070.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAVSYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(23887..24282)
                     /locus_tag="JN331_001915"
     CDS             complement(23887..24282)
                     /locus_tag="JN331_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein kinase
                     BaeS"
                     /protein_id="HAY7260071.1"
                     /translation="MQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAE
                     QHDKTVRLTFADSAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHN
                     GRIIAAHSPFGGVSITVELPLERDLQREV"
BASE COUNT         6229 a         5738 c         6250 g         6104 t
ORIGIN      
        1 gttggccggt ggtggagcag atcttgatgt tagcgtgaaa gaggcggacg gctcggtaac
       61 cacctatctg gtgccttatg cagcggtgcc aaatatgctg caacccggcg tgtcgaaata
      121 tgattttgcg gcgggccgta gccatattga aggggcgagc aagcaaagtg attttgtcca
      181 ggcaggttat cagtatggtt ttaataactt attgacgctg tatggcggca cgatggttgc
      241 taataattac tatgcgttta ccctcggaac gggttggaac acgcgcattg gcgcaatttc
      301 cgtcgatgcc acgaagtcgc atagtaaaca agacaacggc gatgtgtttg acgggcaaag
      361 ttatcaaatt gcctacaaca aatttgtgag ccaaacgtcg acgcgttttg gtctggcggc
      421 ctggcgttat tcgtcgcgtg attaccggac atttaacgat cacgtttggg caaacaataa
      481 agataattat cgccgtgatg aaaacgatat ctatgacatt gccgattatt accagaacga
      541 ttttggccgt aaaaatagct tttctgccaa tatgagccag tcattgccag aaggctgggg
      601 ttctgtgtcc ttaagtacgt tatggcgaga ttactggggg cgtagcggca gcagtaagga
      661 ttatcagttg agttattcca ataactggcg gcggataagc tataccctcg cggcaagcca
      721 ggcgtatggc gagaatcatc atgaagagaa acgttttaat atttttatat caattccctg
      781 tgattggggt gatgacgtta cgacgcctcg tcggcaaata tatatgtcta actcaacgac
      841 gtttgatgat cagggttttg cctcaaataa tacgggatta tcaggaccag tagggagtcg
      901 ggatcagttc aattatggtg tcaacctgag tcatcaacat cagggaaatg aaacgacagc
      961 tggggcgaat ttgacctgga acgcgccggt tgcgacagtg aatggcagtt atagtcagtc
     1021 gagtacttat cgacagactg gagccagtgt ttcagggggc attgtcgcct ggtcgggtgg
     1081 cgttaatctg gcgaaccgtc tttccgaaac gtttgctgtg atgaatgcgc caggaattaa
     1141 agatgcttat gtcaatgggc aaaaatatcg cacaacaaac cgtaatggag tggtggtata
     1201 cgacggaatg acaccttatc gggaaaatca cctgatgctg gatgtgtcgc aaagcgatag
     1261 cgaagcagaa ttacgtggca accggaaaat tgccgcccct tatcgcggcg cggttgtact
     1321 ggttaatttt gataccgatc agcgcaagcc ctggtttata aaagcgttaa gagcggatgg
     1381 tcaaccatta acgtttggtt atgaagtcaa tgatatccat ggtcataata ttggcgttgt
     1441 cggccaggga agccagttat ttattcgcac caatgaaata ccgccatcgg ttaatgtagc
     1501 aattgataag caacaaggac tttcatgcac aatcaccttc ggtaaagaga ttgatgaaag
     1561 tagaaattat atttgccagt aatagtcagg tggttctatg gaaattcgca taatgctatt
     1621 tatattaatg atgatggtta tgcctgtaag ctatgcggca tgttatagtg agttatctgt
     1681 tcagcacaat ttggttgttc aggggaattt tgcacttact caaacacaaa tagcgacata
     1741 tgagcataat tttaatgatt cgtcatgcgt gagtacaaat actatcaccc ctatgagccc
     1801 atcggatatt attgttggac tttataacga taccattaaa ttaaatttac attttgaatg
     1861 gaccaataaa aacaacatca cgttgtcaaa taatcagagc agtttcacca gtggttattc
     1921 ggtaacggtg acacctgcgg ccagtaatgc aaaagtgaat atttctgcgg ggggcggtgg
     1981 ttcagtgatg attaatggtg ttgcgacatt atccagtgct tcatcatcga cacgcgggag
     2041 tgccgcagta caatttctac tgtgtttatt aggtggcaag tcatgggatg catgtgtaaa
     2101 tagctacaga aatgcattgg cacaaaatgc aggtgtctat tccttcaatc tgacattgtc
     2161 atacaacccg ataaccacaa cctgcaaaca ggacgattta ttaattactt tagacagtat
     2221 tcccgtttca caattaccag ccacaggtaa caaagcaaca ataaatagta aaaaaaggga
     2281 tattattctg cgttgtaaaa atttattagg tcaacaaaat caaacatcac ggaaaatgca
     2341 ggtgtattta tcaagttctg acttgttaac caacagcaac acaatactga aaggtgcgga
     2401 agataatgcg taggatttat tcttgaaagt aatggttcgc cagtcacact tttaaatatc
     2461 actaacagca gtaaaggata cacaaattta aaagaaattg cggcaaagtc aaaacttaca
     2521 gatacaacgg tttcaattcc gataacagcc agttactacg tctacgatac aaacaaagtt
     2581 aaatctggcg cactggaggc aaccgcatta atcaacgtga aatacgacta ataacaagca
     2641 agacgagcaa gtggctaata ataaaaaata acaaggtgtc tgcatttccc cctcatgatg
     2701 agggggcttt ttttagcgat gataaaaaat ctcaccgtcg taggctttaa tgattttacc
     2761 gtcggtgtcg ctgatcaaca cgtacgcgcc acccatatag gtccagtgcg tcccggcatc
     2821 aggcgcgggc aggttacgta actgccactg cttaatgttg tactcatcgg tgcggtacat
     2881 ctctgggacg gtatcacctg gtttgaaatg cttaaagtca gcaatgaaac tacttaactc
     2941 atacttagaa attcccgagg tattggtcga acccgcggtg gctggtgcgg cccaggcggc
     3001 actggtaacc agcaaaagca cacccagcaa catcttattt ttaatggtca taaactctcc
     3061 actttgggga tctgtaatat cagtcgcata tctttcctgc catacaatat aactgacaag
     3121 cgatattttg ttagtgatcc catgatttcg taaaaagtgt aaatgaaagg atgccctaaa
     3181 agcatcctta gcgagcagag agtcagcgga aatagctgat ttaagaatca tcctgggctg
     3241 aagataacat gcaagcctaa aggattgagt tatcgcatta tgcccatgaa gccatgtacg
     3301 cccatataca caccgactaa gccaatcaac agactggaaa aatagggggc gcgtttagcg
     3361 agagtgttaa atccgctcca gcgttttgcg acctgctgaa cgctgattgc tgcgccaacg
     3421 cctacggtga aaagcgttaa cgccaggcca atgctgaaac tgacgaccag tgttgcgccc
     3481 agtgtcaggg ctttcaactg aatgcatatc aacagcacgg taattgctgc cgggcagggg
     3541 ataaggccac cggttaagcc aaataacaaa atttgccagt tgatgacctc tctaccatca
     3601 aagcgtcgtt taatgtcatt ggcatggtct cgtgcatggg catcctgata ctcgccatgt
     3661 tcgtgatggt gatgatgtcc ttggtcgtgg tgatgttcgt ggtcttgatg atgttcgtga
     3721 tcgtgatgat ggtgatgatg ctcataatca tgcccgtgca tattctccag ccagttgcgt
     3781 tcgccgcgcc aggtacgcca gaacatccag aacgcggtgc caataatgat cactgcggaa
     3841 atcagctgga gccacggttc tgctgattga gcagtaaagc gcttactgat caccatcccg
     3901 ccaaaggcaa tcaaccagac caccgcggta tgcgaaatgg ttgctgccag tccgagcatc
     3961 accgcctgtt taatggtgcc tttgatggcg atgataaacg ccgccatcat cgtttttgag
     4021 tgccctggtt ccaggccatg aagcgcacca agtaagatgg cgctggggat gaagaaccag
     4081 gcgtttcctt gctgaagaag agttgtaaat tcggtcatga taataattct tagtattaat
     4141 tcggcaatct gattctactc ccccccagta cctgatacta ctcccccagt agattaatag
     4201 tgctatgatt tttcatattc ttgttaacca ggtgttgcca tgtctcatac aatccgtgat
     4261 aaacagaaac tgaaagcgcg tgccagtaag attcagggcc aggtcgtggc gctcaagaaa
     4321 atgctcgacg agccgcacga atgcgctgca gttttacaac agattgctgc tatccgtgga
     4381 gcggtaaacg gtctgatgcg ggaagtgatt aaaggtcatc tgacggaaca catcgttcac
     4441 cagggggatg agctaaaacg tgaagaagat ctggatgtcg ttctgaaggt gctggattca
     4501 tatatcaaat aatttattaa cgcgattgta aaactgccgt ttttcctcgt ttacaacggg
     4561 tgcgctggac attaccatcc tcctctgcga tttatcatcg caaccaaacg actcggggtg
     4621 cccttctgcg tgaaggctga gaaatacccg tatcacctga tctggataat gccagcgtag
     4681 ggaagtcacg gaccaccagg tcattgcttc ttcacgttat ggcaggagca aactatgcaa
     4741 gtcgacctgc tgagttcagc gcaatctgcg cacgcgttac acctttttca ccaacattcc
     4801 cctcttgtgc actgcatgac caatgatgtg gtgcaaacct ttaccgccaa taccttgctg
     4861 gcgctcggtg catcgccagc gatggttatc gaaaccgaag aggccagtca gtttgcggct
     4921 atcgccagtg ccttgttgat taacgttggc acactgacgc agccacgcgc acaggcgatg
     4981 cgtgctgccg ttgagcaagc aaaaagctct caaacacgct ggacgcttga tccagtagcg
     5041 gttggtgcgc tcgattatcg ccgccatttt tgtcatgaac ttttatcttt taaaacggca
     5101 gcgatacgtg gtaatgcttc ggaaatcatg gcattagctg gcattgctaa tggcggacgg
     5161 ggagtggata ccactgacgc cgcagctaac gcgatacccg ctgcacaaac actggcacgg
     5221 gaaactggcg caatacgtcg tggtcactgg cgaggtggat tatgttaccg atggacatcg
     5281 tatcattggt attcacggtg gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc
     5341 attatcggcg gttgtcgctg cctgctgtgc gttaccaggc gatacgctgg aaaatgtcgc
     5401 atctgcctgt cactggatga aacaagccgg agaacgcgca gtcgccagaa gcgaggggcc
     5461 aggcagtttt gttccacatt tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc
     5521 atgaaacgaa ttaacgctct gacgattgcc ggtactgatc cgagtggtgg tgcggggatt
     5581 caggccgatc ttaaaacctt ctcggcactt ggcgcttatg gttgctcagt tattactgca
     5641 ctggtggcgc aaaatacccg tggcgtacag tcggtgtatc gcattgagcc tgattttgtc
     5701 gccgcccagc tcgattcggt gttcagcgat gtgcgaatcg ataccactaa aatcggtatg
     5761 ttggcggaaa ccgatattgt tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa
     5821 aacgtggtac tcgacaccgt tatgctggca aaaagcggcg acccgctgct ttcaccttcg
     5881 gcggttgcta cgctgcgcag tcgattattg ccacaggttt cattaataac gccaaacttg
     5941 cccgaagctg ccgccttgct cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa
     6001 caagggcgat cgctgttggc gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg
     6061 gatgatgagc aaagcccgga ctggctgttt acccgcgagg gtgaacaacg gtttaccgca
     6121 ccgcgcatta tgaccaaaaa cacccacggc actggttgta cactctctgc ggcgttggct
     6181 gcactacgcc cgcgccatac aaactgggct gacaccgtac aggaggcaaa aagctggctt
     6241 tcatcggcgt tagcccaggc cgacacgctg gaagttggtc acggtattgg tccggttcac
     6301 cacttccacg cctggtggtg agcaccgtac tatagctgct ttggttattg tcagcctttg
     6361 gcactgacat tcagggatga aaccaatgca gctaagaatt accagtcgaa aaaaactcac
     6421 cgctctatta tgcgctcttg ggctaatttc catcgttgca atttatccgc gtcagacggt
     6481 gaattttttc tattcgacag cagttcagat tacagactac atccacttct acggttatcg
     6541 cccggttaaa tctttcgcta ttcgcattcc tgccagttac accattcacg gattagatgt
     6601 ttcgcgctgg caggagcgta tcgactggca gcgggtggca aaaatgcgcg acaacgatat
     6661 ccgcttacag tttgctttta ttaaggctac ggaaggcgaa aagctggtgg acccctattt
     6721 ttcgcgtaac tggcaattaa gccgcgaaaa tggcctgctg cgcggggcgt atcattattt
     6781 ctccccgtcg gtatccgctt cagttcaggc gcgattattt ctgcaaacgg tggatttctc
     6841 acaaggcgat ttccctgccg tgctggacgt agaagaacgg ggaaaattat cggcaaaaga
     6901 attacgcaag cgggtaagtc agtggctaaa aatggtcgaa aaacgcacgg gaaaaaagcc
     6961 gattatttac tcaggagccg ttttttatca caccaatctg gcgggctatt tcaatgaata
     7021 tccgtggtgg gtggctcact attatcaacg tcgtccggac aatgacggca tggcctggcg
     7081 cttctggcag cattccgacc gtggacaggt agatggcatc aatggtccgg tggattttaa
     7141 tgtgtttaat ggcacggtgg atgagttgca ggcattcgtt gatgggatta aagaaacgcc
     7201 ttaaataaga agcagtgata tactcccacc agactccctg gcaggaaaaa gacaaatgga
     7261 acaagcgcat acccagctga ttgcccaact gaacgagcgt attttagcgg cggataacac
     7321 gccgctttat attaagtttg ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga
     7381 gcatggcaat attttgcccg gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat
     7441 tacggtgcgc aaggcgatgc aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg
     7501 ttacggcacg cagatcaaca acatcttcga atactcgttg aaagaagcgc gaggtttttc
     7561 tcagcaggtg gtattgcgcg ggaaaaagcc cgatacgcta tgggttaata agcgtgtcgt
     7621 gaaatgtccc gaagaagtcg cgcagcagct ggcggtcgaa gcgggaagtg atgtcttttt
     7681 gcttaagcgt attcgctatg tcgatgaaga agcggtatcg attgaggaat cgtgggttcc
     7741 ggcgcattta attcatgatg ttgatgccat cggaatttct ctttatgact atttccgcag
     7801 ccagcacatt tacccacaac gtacgcgttc ccgcgttagc gcccggatgc cggatgccga
     7861 gtttcagtca catattcagt tagatagcaa aataccggtg ctggtgatca agcaagtggc
     7921 gcttgaccaa cagcaacggc ctattgagta cagcattagc cactgtcgca gcgatctata
     7981 cgtttttgtg tgcgaggagt agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc
     8041 cgacaaccca cgacgccact gcattgccca gtaatacggc atccgccagt ggcagaccag
     8101 aggccagccc ggcaagtacg ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa
     8161 cttgcgttgg aaatgccgga atgcagccag aagcgtcgtt gctgaaatac catgcgcctt
     8221 ctttatcgag acgaacgatc aacggcgcgg caaatttctc ctgccattgc ttgccaagtg
     8281 ttgttatctc tgcggataaa gcaaaacgtt cggcggcaat ctcagcctct tgacgattga
     8341 gtgacactaa aggtcgacag gccatgatcc gcgccagtaa tgcgtccggg atatcgccaa
     8401 tacgtgggcc aaaatcgata aacggcgtca cgtcttgcag cttttccagc cattccacta
     8461 acaattcgcc gcagggcgag gccagttgat aaccggaaaa atagagcagg ttgccaggcg
     8521 caacggttaa tcgtgccaac cattggcgat tccattgatt ttccacaccg ctgaatgaca
     8581 taaaagtgcg ttcgccatcc ggctcaacca gtgccagaca ccagccgtta tcgctttcgg
     8641 cgttatcgat caggctgatt aacccctctt tcgccatccg gttgcgaata atctccgccc
     8701 acacgccctg accgagcggc aaggcattac ccgcttcgat gccgaggcgc tttaacgcca
     8761 cggcaatatt cagtgcgcag ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc
     8821 acccacgcca ggggagggca taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga
     8881 ccatcaccgg ctgacgcgtg gttaattcag gcagcagtgt gtgtaatcga gcgccgctca
     8941 taccgcctcc cgttgttgac gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag
     9001 ctgatttacc gcatccagct ccgcctttaa cgcaggatcg acagcgttaa tgccatgcaa
     9061 cgcgccgcaa atcgccgtcg ccatagcacc tatggtgtct gtgtcgccgc caagattggc
     9121 gcacaggacg gcgcagcggt ttgggtcggt ttgtgccagt tcaactagcg caatggcgca
     9181 cggaacggac tcaatagtgc tggtgcctgc gccaacgact tggtaaagct gttcgctggc
     9241 ggattcggtg ccgtcggcat tacgcacaat tttcagcgca atctccagac gtgccgccag
     9301 tgaggcgctg aatgtggtgg tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaatga
     9361 atcaacaatc gctgaccagc gttctccgtc aatggcacga gaaatcgccc atgcgatgac
     9421 taccgcgcct gcaaccgcca gatcggattt atgggtcggg ctggacgcca gcgccacatc
     9481 atcaataaag gaatcaacat cacgcgctgg aagcaaacaa cctaatggcg agacgcgcat
     9541 cgctgcgccg ttggttacgc cgttgttttc cagttcagca acgggtttac cgtcgcgaat
     9601 ggcgttaagc gcaatcttcg aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg
     9661 caacgcccag tcgagaatat tacgcccaat cagattcgga tcgatcttgc cttcacattc
     9721 cagtaacgca tccgccagac acaggccatc gaggtatcgt cggtgaattc ggcgcggtta
     9781 aaataacagg ccgcgttatt ctcctttggt ccaggcagaa aacggtcaat ccagccaaag
     9841 tgtgctttaa cgcggctgcg tggccaaagc tcggagggca tccccatcgc atcccctaac
     9901 gcctgcccat aaagagcacc gagaatacgt tctgttttca tttaacttcc ccttgtgtca
     9961 acgcaatatc tcgatcatcg accttgatag ccgtaatttc gttgtcggat tcgcggaaaa
    10021 aaatcatgaa cagcacggcg ataatggcaa tcatcaccgc gccgaaagtc cacatcccgg
    10081 accagttgaa agtcagtccg tttaccggtt cctgataagc gaacatcttt tccatcatca
    10141 caccgccaag acgatagccg agcaaactgc cgaagccttg gcagcagagc gtgatcagcc
    10201 cctgcgcagc ggtacgcata tgcacggggg cttttttatc gacataggtg taagcggtaa
    10261 cgtagtaaaa atcgtaactt acgccgtgaa gcaaaatacc gaggaacagt aacgcgtagg
    10321 tgaaatattc atccgcacta ccgtaaataa agaagccata gcagatcgca gcggtgacca
    10381 gaccaagcaa taataccttt ttgataccaa agcgtttagt gaaaaacggc aatgccagca
    10441 taaagaagat ttcagagaac tggccgagcg tcatccagcc ggtggcgttt ttcatgccaa
    10501 cttcggtcag ataaccgttg gcaaagatgt aatagaacgc cagtggcatc gcaaacagga
    10561 atgaacagaa gaaaaagacg aggaagtttt tatcgcgcag caggatcagc gcatccaggc
    10621 cgagcatgac tttaatgtcc attttgccgg tgctttttgg cggcgtgttg ggcaggaaaa
    10681 acgcaaacac accgagcaga gcagaacttc cggcggtaat tagcagcggg atgttagtcg
    10741 gtgagatatc ggcatacccc agcatttgcg gcaagaaacc acatgccaga ccggaggcaa
    10801 tccagccgat agtgcccatc acacgaatgc gtgggaaatc acgttccaca tccggcacgt
    10861 tggcaaaagc gatgctgtta gtcagcgcaa tggtcggcat ataggttagc gagtaggcca
    10921 gcagtaacgg aaagaacccg gcaaaagtgg tctgttgcgc agcgaaatac atcagcacag
    10981 caccagcgaa catcaatacc gccagcactt tctgcgccga gaaaaagcgg tcagtgatgg
    11041 agccaaccag aattggcgac aggatcgccg caatggctgt gcaggcatac gaccagccaa
    11101 tttctccggc actaaaaccg cttttactta accacaacca caatggcaca aaccacgcgc
    11161 cccagataaa ccattcaaca aacatcatga acgacagctt tgctgttgtt ttcatctttt
    11221 aattccttca tgacaggtaa tgtaaggtac gcgatgacaa taccacctaa taatgccttt
    11281 tgaatacctt tgcggtgaaa acttgaccgc gctaacattt tttctgatga atcgagccaa
    11341 cagaaaacgc tgaaaaaaca tccaaaagat ggaaaaactc gcccgtaacg accaggatta
    11401 tgattgcccg caaaaggcgg gccaggacaa tccatagccg atatccaatc ggaatttacg
    11461 ggagcatagt aatgacagat attgcgcagt tgcttggcaa agacgccgac aaccttttac
    11521 agcaccgttg tatgactatt ccttctgacc agctttatct ccccggacat gactacgtag
    11581 accgcgtgat gattgacaat aatcgcccgc cagcggtgtt acgtaatatg cagacgttgt
    11641 ataacaccgg gcgtctggct ggcacaggat atctttctat tctgccggtt gaccagggcg
    11701 ttgagcactc tgccggagct tcatttgctg ctaacccgct ctactttgac ccgaaaaaca
    11761 ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc atcaacttac ggcgtgttgg
    11821 tgtcggtatc gcggcgctat gcgcatcgca ttccatttct cgtcaaactt aatcacaacg
    11881 agacgctaag ttacccgaat acctacgatc aaacgctgta tgccagcgtg gagcaggcct
    11941 tcaacatggg cgcggtggcg gttggtgcga ctatctattt tggctcggaa gagtcacgtc
    12001 gccagattga agaaatttct gcggcctttg aacgcgcgca cgagctgggc atggtgacag
    12061 tgctgtgggc ctatttgcga aactccgcct ttaagaaaga tggcgttgat taccatgttt
    12121 ccgccgacct gactggtcag gcgaaccatc tggcggcaac cataggtgca gatatcgtca
    12181 aacaaaaaat ggcggaaaat aacggcggct ataaagcgat taattacggt tataccgacg
    12241 atcgcgtgta cagcaagtta accagcgaaa acccgattga tctggtgcgt tatcagttag
    12301 ctaactgcta tatgggccgg gctgggttga taaactccgg cggtgctgca ggcggtgaaa
    12361 ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa acgcgcaggc ggaatggggc
    12421 tgattcttgg acgtaaggcg ttcaagaaat cgatggctga cggcgtgaaa ctgattaatg
    12481 ccgtgcagga cgtttatctc gatagcaaaa ttactattgc ctgatacgcc tcatacgaac
    12541 agcaccaccc cgtaggacgg ataaggcgtt tacactgcat ccggcaacct atgcctgatg
    12601 cgacgcggaa gcgtcttatc atgcctacat agcactgcca cgtatgttta caccgcatcc
    12661 ggcataaaaa cacgcgcact ttgctacggc ttccctatcg ggaggccgtt tttttgcctt
    12721 tcactcctcg aacaattttc atattgtcgt ttttgtgatc gttatctcga catttaaaag
    12781 caaataattt cattatattt tgaaatcgaa agcaaacgac aagatatgaa aatgtacgtg
    12841 gtatcgacaa agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc
    12901 aatattcaca atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg
    12961 ccggtcatca tcgccggaac gcctggcaca tttactcatg ctggtacaga aaatctgttg
    13021 gcgctggtca gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat
    13081 cacacgaaat ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt
    13141 gacgcctcgc atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt
    13201 tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc aacttggcgg ccaggaagat
    13261 gatgtgcaag tcaatgaagc cgatgcgttg tacaccaacc ccgcacaggc gcgtgaattt
    13321 gccgagggat tgattccctg gcggtcgcca tcggcacggc tcacggtatg tatgccagcg
    13381 caccggtgct tgatttttct cgactggaga acattcgcca gtgggtgaac ttaccgctgg
    13441 tgctgcatgg cgcgtcagga ttatcgacta aggatattca gcaaaccatc aaactgggga
    13501 tatgcaaaat caacgttgca acggagctga aaaatgcctt ctcgcagtcg ttaaaaaatt
    13561 acctgaccga gcaccccgaa gcgaccgatc cccgggatta tttgcagtcg gctaaatccg
    13621 caatgcgcga tgtggtgagc aaagtgattg ccgattgtgg ctgcgagggc agggcataac
    13681 gcacctgcca tttaacaagg aaaaaacatg aaaacgttaa ttgcccggca taaagctggt
    13741 gaacatatcg gcatatgttc agtctgttct gcccatccgt tggttatcga agcggcgctg
    13801 gcatttgatc gcaacagcac gcgcaaagtg ctgattgaag caacgtcaaa ctaggtcaat
    13861 caatttggcg gttataccgg aatgacaccg gcagactttc gcgaatttgt ttttacgatt
    13921 gccgataaag ttgggtttgc acgcgaacgc attattctcg gcggcgatca tctggggcca
    13981 aactgctggc agcaagaaaa tgcggatgcg gcgatggaaa aatccgtcga gctggtaaaa
    14041 gcatatgttc gtgctggctt cagtaaaatt catcttgatg cgtcaatgtc ctgcgcgggg
    14101 gatcccatac cgttagcacc agaaacggtt gcggaacgag ctgctgtgct ttgctttgct
    14161 gcggaaagtg tggcgacaga ttgccagcgt gagcaactga gctatgtcat tggcactgaa
    14221 gttccggttc cgggcggtga ggccagcgcc attcagtcag tacacatcac ccatgttgaa
    14281 gatgccgcca atactttacg tacgcatcaa aaggccttta ttgcccgtgg gctgacagag
    14341 gcgttaacac gtgtgattgc catcgtggtg cagccgggtg tggaatttga tcacagcaat
    14401 attatccatt atcagccgca ggaagcgcag gcgctggcgc aatggataga aaacacccga
    14461 atggtttatg aagcacattc taccgattac cagacccgga cggcttattg ggaattagtc
    14521 cgcgatcact ttgcaatatt gaaagtcggt cccgcattaa cctttgcttt acgcgaggcg
    14581 atatttgcac tggcacaaat tgagcaggaa cttatcgccc ccgaaaatcg cagcggttgc
    14641 ctggcggtaa ttgaagaagt gatgctcgac gaaccgcaat actggaaaaa atattatcgc
    14701 acgggtttta acgattcatt actggatatt cgttacagcc tgtcggatcg tattcgttat
    14761 tactggccgc atagtcggat taaaaatagc gttgaaacga tgatggtgaa tctgcaaggc
    14821 gtggacatcc cactgggcat gattagtcag tatcttccca aacaatttga acgcattcag
    14881 tccggggaat tatcagcaat gccgcatcag cttattatgg ataaaattta tgatgttttg
    14941 cgcgcctatc gctacggctg tgcggaataa ggacggtata tgactaacct gtttgttcgt
    15001 agcggaattt cttttgtcga tcgtagcgaa gttttaacct atatcggtaa tgagatgctc
    15061 gcgaaaggtg tggttcatga tacctggcca caggcattaa ttgccagaga agcagaattc
    15121 cctaccggga taatgcttga gcagcacgcc attgcaatac cgcattgtga ggcgattcat
    15181 gctaagtcgt cagccattta tctgttaagg ccaacaaata aagttcattt tcagcaagcg
    15241 gatgatgata acgacgtggc ggtatcgttg gttattgcgt tgattgtgga aaatccgcag
    15301 cagcaattga aacttttacg ctgtttattt ggcaagttac aacagcccga tatcgtcgag
    15361 acactaatta ctcttcctga aactcagtta aaggaatact tcacaaagta tgttttagat
    15421 tcagacgaat aaatccctct gtaacaataa aaaggactat ttatgaaacg caagattatt
    15481 gtcgcttgcg gaggcgcggt tgcgacctct acgatggcgg cggaagaaat taaagagttg
    15541 tgtcagagtc ataatattcc tgttgaatta atccagtgtc gggttaatga aatagaaacc
    15601 tatatggatg gtgtgcattt gatatgtacc actgccaggg tggatcgtag ttttggcgat
    15661 attccgttag ttcacggcat gccttttgtt tctggtgtcg gtatcgaagc attacaaaat
    15721 aaaattctga ctatcttaca ggggtgacct atgttttcag aagtcatgcg ttatattctc
    15781 gacctcggcc ctacagtgat gctgccggtt gtcatcatta ttttttctaa aatattaggc
    15841 atgaaggcag gcgattgctt taaagcgggt ctgcatatcg ggattggctt tgttggcatt
    15901 ggccttgtga ttggcttaat gctggattcc attggtccgg cggcgaaagc gatggcggaa
    15961 aatttcgacc tgaatctgca tgtggtcgat gtcggctggc cgggctcttc accaatgacc
    16021 tgggcgtcgc aaattgcgct ggtggcgatt ccgattgcga tattggttaa cgtggcgatg
    16081 ttactgaccc gtatgacgcg ggtggtaaat gttgatatct ggaatatctg gcatatgacc
    16141 ttcaccggcg cgttgctgca cctggcaacc ggttcatgga tgatagggat ggcgggtgtg
    16201 gtaattcacg cggcgtttgt ttataagcta ggcgactggt ttgcccgcga tacccgaaat
    16261 ttctttgagc tggaaggtat tgctattccg cacggtacgt cggcgtatat ggggccgatt
    16321 gcggtgctgg tcgatgctat catcgagaaa atcccaggcg ttaaccgaat taaatttagc
    16381 gccgacgata ttcagcgcaa atttggccct tttggcgaac ctgtcaccgt gggttttgtg
    16441 atggggctga ttatcggcat cctcgcgggt tacgatgtca aaggtgtatt gcagctggcg
    16501 gtaaaaacgg cggcagttat gctgctgatg ccacggatga ttaaacccat catggatggt
    16561 ttaacgccca tcgctaagca ggcccgtagt cgtttacagg cgaagttcgg cggtcaggag
    16621 ttcctgattg gcctggatcc cgcattactg ctggggcata cggcggtggt atcggcaagc
    16681 ctgattttta ttccgctcac cattttaatt gctgtttgtg ttccgggtaa tcaggtactg
    16741 ccgtttggcg atcttgccac aatcggcttc ttcgtggcga tggcggtcgc ggtgcatcgt
    16801 ggaaatctgt tccgcacctt aatctcgggt gtcatcatta tgagcatcac cctgtggatc
    16861 gcgacgcaaa ctattggttt gcacacccaa ctggcggcta atgctggggc gttaaaagcc
    16921 gggggtatgg tggcttcaat ggatcagggc ggttctccca ttacctggtt actgattcag
    16981 gttttctccc cgcaaaatat tcccggtttc attattatcg gcgcaattta tctgaccggt
    17041 attttcatga cctggcgtag agcgcgtggt tttactaaac aagagaaagc cgttctcgca
    17101 gaataatttt tacctgaggg ggaggttatc ccccttaaca atcaggagtt tttatgaaat
    17161 caggagtttt tatgaaatca gtagtgaatg atactgatgg tatcgtgcgc gttgcaaaaa
    17221 gcgtcattcc tgaaattaaa catcaggatg aggtgcgggt aaaaattgcc agctcgggct
    17281 tatgtggttc cgatttaccc aggatattta aaaatggtgc acattattat ccaataacgt
    17341 taggccatga atttagcggc tatattgatg cggtgggatc cggtgttgat gatttacacc
    17401 ctggcgatgc ggttgcctgt gtgccgttat taccctgttt tacttgtcca gagtgtttga
    17461 aagggtttta ttcccagtgc gcaaaatatg attttattgg ctcgcggcgt gatggtggat
    17521 ttgctgaata tattgtcgtt aagcgaaaaa atgtctttgc tctacccacg gatatgccta
    17581 ttgaggatgg ggcttttatt gagccgatta ccgttggcct gcatgctttt catttagcgc
    17641 aaggttgtga gaataaaaac gttattatta ttggtgccgg aaccattggc ctgctggcta
    17701 ttcagtgcgc tgtcgcgctg ggagcaaaga gtgtgacggc gatcgacatt agctcagaaa
    17761 aactggcact ggcaaaatct ttcggtgcga tgcaaacatt taacagtagc gaaatgagcg
    17821 cgccgcaaat gcagagcgtt ttacgcgaac tgcgctttaa tcagcttatc ctcgagacgg
    17881 ctggcgtacc gcaaactgtc gaactggcgg tagagattgc cggtcctcat gcccaactgg
    17941 cgctggtggg cacgttgcat caggatctgc atttaacatc ggcaacgttt ggcaaaatat
    18001 tgcgtaaaga gctgacggtt atcggcagtt ggatgaacta ttccagccct tggccggggc
    18061 aggagtggga aacggcgagc cggttgctga cagaacgtaa gttaagcctg gatccattaa
    18121 tcgctcaccg tggaagcttt gaaagcttca cccaggcggt gcgtgacatc gctcgtaatg
    18181 ccatgccggg caaagtgttg ctcattccat gaaaccgcgg gccagcgtga tgctggcccg
    18241 gtattgtgca aaacagatca ttcaccaatg gtcccccttc gtttacacta gccacaattg
    18301 aatatgggta aatgacgatg aattcattcg agcgaaggaa taagatcatc caattagtga
    18361 atgaacaggg aaccgtgctt gttcaggatc tggcgggagt atttgctgcc tcggaagcga
    18421 caatccgtgc cgatttgcgc tttctcgaac aaaaaggcgt ggttacgcgc tttcatggcg
    18481 gtgcggcgaa aataatgtcc ggtaatagtg aaaccgagac ccaggaagtc gggtttaaag
    18541 agcgatttca gctcgccagc gcgccaaaaa acagaatagc gcaggcggca gtcaaaatga
    18601 tccatgaagg gatgaccgtt attctcgaca gcggaagtac cacaatgctt atcgccgaag
    18661 gattaatgac cgctaaaaat atcacggtga ttaccaacag tctcccggca gcgtttgccc
    18721 tttccgaaaa taaagacatt actctggtcg tctgtggtgg cacggtccgc cataaaacgc
    18781 gctcgatgca tggttccatt gccgagcgtt cattgcagga tattaatgcc gatttaatgt
    18841 ttgtcggtgc tgatggtatt gatgcggtta atggtattac gacctttaat gaaggttatt
    18901 cgattagcgg ggcgatggtc accgccgcaa ataaagtgat tgccgttctc gattcatcga
    18961 aatttaatcg tcgcggtttt aatcaggtac tgccaatcga aaaaattgac attattgtta
    19021 ccgatgatgc tgtgtcagag gtggataaat tggcattgca gaagacacgg gtaaaattaa
    19081 ttacggtgta gtattgttct gtatgccgga taaggtgttt acaccgcatc cggcattacg
    19141 gattctccct ggttcaagag agaatagttt gattaacgca ataatggaca atctggtggt
    19201 aatcgacaac gcaacgccgc cggaagtatt tcaatatgga agttttgccc actcaacggt
    19261 tcgccatcaa gattaaaagt gatttcgtgt ggggcttgta tatcaaacca cgacgaagcg
    19321 ccttcgataa tattcgggtt atcttcgtca gattttaagg ttgatacgag agccggaaga
    19381 atttcatcgc cggtaaaaat gcgcagttgc agcaagccat cgttaattaa cgcgttcggg
    19441 cacaattgtt gaccgccacc ggcctgacgc ccgttaccaa taccaatgac cagggcgtca
    19501 ccttgccagt gaaagttttc accgcggatt tcacaacggt ccggttgcag agtatccatg
    19561 cgcattaagc catgaatgat gtaagagacg ccacccagcg cggcttttaa tttttccggc
    19621 gtttctgtgg taatacgcgt cccaaatccg cctgtcggca tattaataaa acaggtttgt
    19681 ttgttgacct gcgccatatc tatcgcaatg gcattaccgg caattgccag tttcagcgcc
    19741 ttatccagtg cctcaggaat ccctacactg gtggcaaaat cattggcggt tcctaatggc
    19801 aaaattccca gcgcgggtat gtcatccccc tcacactgaa tcaacgccgt agaaacttca
    19861 ttaatggtgc catcgccacc accggcaatc accgttgcga cgcccaactt ccgggcctcc
    19921 tctacaaatc gtgcggcatc acctttctcc caggtgaccc gcacatggat cgtcattcct
    19981 tcctcacgca acagcataat tgcttcgcgc aagggtagat tgtcagtact tttgccatta
    20041 agaatcagta agctggcggg aaattctgcc atgatcgtat gtgcctttat gattggtctg
    20101 tagatagtgt agagcagaaa acaaaaggtg gagtcagaac aggatgaaag tcggaggata
    20161 aaaagtcagt tcaagtatgg aggattcagt ggttaccacc aggagcttgc aatggggaca
    20221 gcgaatacta acggctgcca ccgataaatt tcaaaaaaga gcatatacct aatattcaac
    20281 taaacagtgg catcttcaat ataatatatt aaagccccca tggagttacc ctgaagggcc
    20341 tcaatgtccg taattcctac ttatgtagga aatgttgtac agaacattta ttataatcct
    20401 attcaaatat aataatcatg ccattattat atttaaacac tagagagtgt cgttggtatt
    20461 taatggggga aggtgagatg aaaaagatag ctgctatatc attaattagt atttttctta
    20521 tatctggttg tgctgtgcat aatgatgaaa caagtatcgg taaatttggt cttgcatata
    20581 aaagtaatat tcagcgtaaa ctcgataacc aatactacac cgaagccgaa gcttctttag
    20641 ccaggggcag aatatctggt gcagaaaata tagtaaaaaa tgatgcagcc catttctgtg
    20701 ttactcaggg caaaaaaatg cagatagttg acctgaagac agaaggtgca ggattacatg
    20761 gcgtcgctcg tctgacattc aaatgtggag agtgagaata ttttttggta agcgtcaaac
    20821 atgcgcgttc tggctgtgcg tagccggaac ctgggcgagc acgataccgt tacgtgaaag
    20881 gcattgtgct atgaaggggg attctatcga tgtggtcaat ggaagacggt tatcagggat
    20941 caatccttct ttacagctta tgtacaaaaa ataagcccgt gtaagggaga ttacacaggc
    21001 taaggaggtg gttcctggta cagctagcat tttatgggtt atgtttttca gcgaaacgga
    21061 tgataacctt aataaatgca gctgtatgtg atcggtttct aagaattttc catccgggaa
    21121 aaataatcga aattaatcac ttaccgtggg ggttacgcgt ggtttccccg gagaaattac
    21181 gcattagcag cgcgtaattt agctcaagat cctgcgggac cgggagccac acagtataac
    21241 catcgcctgg tgcgaccggc atagcttcgc ctttggcgtt ttccatgtgc tcaagggtaa
    21301 agttaatgtt gccttgcggc gtcatcagct caagactgtc gccaacggag aatttatttt
    21361 tcaccgctac cgctgcgagg tcccccttgc gctcaccggt aaactcacca acaaactgct
    21421 ggcggtcaga aactgaataa ccgtattcgt agttctgata atcgtcgtga gtatgacgac
    21481 gcaggaaacc ttcggtatag ccacgatgcg ccagaccttc cagagtttcc agcaggctgg
    21541 tatcgaacgg ttttcccgca gcggcgtcat cgatagcttt gcggtaaacc tgtgcggtgc
    21601 gtgcgcaata gtagaaagat ttggtacgac cttcgatttt cagcgaatgc acgcccattt
    21661 tggtcaggcg ttctacatgg gcgatggcgc gcagatcttt cgagttcatg atgtaagtgc
    21721 cgtgctcatc ttcaaacgcg gtcatatact cgcccggacg ctgggcttct tcgatcataa
    21781 acactttgtc ggttggcgcg ccgataccca gcgtcggctc aacattttgc accggaatcg
    21841 gctcgtactt gtgtacgatg ttgccaacgt catcttcttt cccttcctgg acgttgtact
    21901 cccagcggca ggcgttggtg caggtgccct ggttcgggtc gcgcttgttg atatagccag
    21961 agagcaggca gcgaccggag taggccatgc acagtgcgcc gtgaacgaag atctcgatct
    22021 ccatatccgg cacctgatta tggatctctt caatctcttc cagcgacagc tcgcgagaga
    22081 ggatcacgcg ggtcagtccc atttgctgcc agaatttcac cgtcgcccag ttcacggcgt
    22141 tagcctgcac cgagaggtgg atcggcattt cagggaagtg ctcacgcacc agcataatca
    22201 gccctggatc ggacataatc agcgcatccg gccccatttc caccaccggt ttcaggtcac
    22261 ggataaaggt tttcagcttg gcgttgtgtg gtgcaatgtt gaccacgaca taaaactttt
    22321 tccccagcgc gtgggcttca ttgatgccga gctgaagatt ttcgtggttg aattcgttgt
    22381 tgcgcacacg tagggagtaa cgcggctggc ccgcataaac agcatctgcg ccataagcga
    22441 aagcgtaacg catatttttc agcgttcccg ccggggaaag gagttccggt ttaaacataa
    22501 ttctctcgtt ctgatgacag gtcagatccg cttcacctga tgaagcggtt aggggaggcc
    22561 cccactttaa gggcgggcat tgtagcgctg taggggggat ctggtaaagc gccggacgcc
    22621 aacaacccac gcccggcatc gtaattacgt attgtctttc accgtctggc ttgtgccatt
    22681 ggctttaacg gaggcaattc cggtttcacg agattgcgcg gtggcgtaca tctggctgga
    22741 gccgataatt tgatgattag cggctttcag attgaaatag aatttgccgt tgcttgccgt
    22801 ttttttctca tagcgctctt cttgcgggct gttgctacgc accgacgcga tgcccttttc
    22861 cgcagaggct tttgaggtat acagctcgct ggtgaggata gtctcaccat tgcccgcttt
    22921 tagcacaaac cggaactgat tatcactgct cttactgagt tcaaaccaac cagccataat
    22981 gactccttga ttgaaattgc ttatcgaaaa cagcatctaa aggtgctgtt tactgattaa
    23041 agtatgagcg attttctgat gcctgccagg gcggagagat aatgtcagtt tgctgaccat
    23101 gcgtggaata atgttgccgg atcgggtctg aacgccatct ccggctaaca aagtaatgtc
    23161 gctaaaacta aacgatgcgg caggcgtcgg cttcccagcg gtaaccaacg ccgtaaacgg
    23221 cgcggataaa tgactgttcg gcgtcgagag attccagctt gcggcgcaga tttttaatgt
    23281 ggctgtcaat ggtgcggtcg gttactacgc ggtagtcgtc ataaagatga ttgagcaatt
    23341 gctcgcggga gaacactttt cctggttcgt gagagagcgt tttaagcaga cgaaattccg
    23401 caggcgtcag gtcaagcatt ttaccgcgcc atgaagcctg aaaacgacct tcgtcgataa
    23461 tcaacgggct ttcggcatcc tgttgctgaa gttcgcgttg cggtttacaa cggcgcaaaa
    23521 tggttttgac acgcgctacc acttcccgtg ggctgtacgg cttgcagatg tagtcatcgg
    23581 cgccaatctc cagccccagc aggcggtcga tctcttcgat ttttgccgtc accatcacga
    23641 tcggaacatc ggaaaaacga cgaatttccc ggcataatgt caggccatcg gtgccaggga
    23701 gcatcagatc caacaggatc agatccggtg gtgtctggcg cacatacgcc agtacctgat
    23761 cgccgtggct gataagcgtc ggcgcatagc tcacagcacg cagataatca atgagcaact
    23821 gccccagctt cggttcatct tccacgatca aaatacgcgg tgtgttttcg tcgattggta
    23881 actcggtcat acttctctct gtaaatcccg ttccagcggt aactctactg taatgcttac
    23941 cccgccaaaa ggcgaatggg cggcaataat gcggccatta tgggcttcaa caatgttcag
    24001 gcaaatcgcc agccccagcc cggaaccgcc gctggcgcgg ttgcgggaac cttcggtgcg
    24061 ataaaaacgt tcaaacaatt tttgtagctg atcgtcactg acacctggcg cactgtcggc
    24121 aaaggtcagg cgcaccgttt tgtcgtgctg ctcggcagag attttcaggc tgccgccgct
    24181 gtcagtgtag cgcaggctgt tttccagtaa gttattgaat aactgcatta aacggtcgcg
    24241 atcgccaaat acggtgatac tgtctggcag ggaaaattgc agtttcaggc cacggctggc
    24301 gaatcgttcg cgaaatgcgc c
//
LOCUS       DADLSB010000049        23859 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.049, whole genome shotgun sequence.
ACCESSION   DADLSB010000049 DADLSB010000000
VERSION     DADLSB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.049"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1095
                     /locus_tag="JN331_001916"
     CDS             <1..1095
                     /locus_tag="JN331_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7260072.1"
                     /translation="VASPAQASVSDLTGDQPAAQPVPVSAPATSTAAVSAPANPSAEL
                     KIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEELLKSNTPLNVLALNQPENIENR
                     VNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSALGDRVANAFAQEWQKLGGGTV
                     LQQKFGSTSELRAGVNGGSGIALTGSPITPRATTDSGMTTNNPTLQTTPTDDQFTNNG
                     GRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFRLEMEGLQY
                     SEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQVQGFEINGN
                     TGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            1053..1448
                     /locus_tag="JN331_001917"
     CDS             1053..1448
                     /locus_tag="JN331_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY7260073.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            1468..2058
                     /gene="diaA"
                     /locus_tag="JN331_001918"
     CDS             1468..2058
                     /gene="diaA"
                     /locus_tag="JN331_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY7260074.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            2068..2643
                     /gene="yraP"
                     /locus_tag="JN331_001919"
     CDS             2068..2643
                     /gene="yraP"
                     /locus_tag="JN331_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY7260075.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(2757..3797)
                     /locus_tag="JN331_001920"
     CDS             complement(2757..3797)
                     /locus_tag="JN331_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7260076.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVTMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(3870..4505)
                     /locus_tag="JN331_001921"
     CDS             complement(3870..4505)
                     /locus_tag="JN331_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY7260077.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            4633..5151
                     /gene="yhbO"
                     /locus_tag="JN331_001922"
     CDS             4633..5151
                     /gene="yhbO"
                     /locus_tag="JN331_001922"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY7260078.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLATSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(5131..5563)
                     /locus_tag="JN331_001923"
                     /pseudo
     CDS             complement(5131..5563)
                     /locus_tag="JN331_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
     gene            5614..5916
                     /gene="yhbQ"
                     /locus_tag="JN331_001924"
     CDS             5614..5916
                     /gene="yhbQ"
                     /locus_tag="JN331_001924"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY7260079.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(5903..6406)
                     /locus_tag="JN331_001925"
     CDS             complement(5903..6406)
                     /locus_tag="JN331_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7260080.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(6400..6924)
                     /locus_tag="JN331_001926"
     CDS             complement(6400..6924)
                     /locus_tag="JN331_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY7260081.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            7133..8128
                     /locus_tag="JN331_001927"
     CDS             7133..8128
                     /locus_tag="JN331_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7260082.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDDYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            8137..9015
                     /locus_tag="JN331_001928"
     CDS             8137..9015
                     /locus_tag="JN331_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7260083.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            9096..10103
                     /locus_tag="JN331_001929"
     CDS             9096..10103
                     /locus_tag="JN331_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HAY7260084.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(10221..11465)
                     /gene="mtr"
                     /locus_tag="JN331_001930"
     CDS             complement(10221..11465)
                     /gene="mtr"
                     /locus_tag="JN331_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY7260085.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(11619..13508)
                     /locus_tag="JN331_001931"
     CDS             complement(11619..13508)
                     /locus_tag="JN331_001931"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY7260086.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGECREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(13501..13581)
                     /gene="yrbN"
                     /locus_tag="JN331_001932"
     CDS             complement(13501..13581)
                     /gene="yrbN"
                     /locus_tag="JN331_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY7260087.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(13688..14572)
                     /gene="nlpI"
                     /locus_tag="JN331_001933"
     CDS             complement(13688..14572)
                     /gene="nlpI"
                     /locus_tag="JN331_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY7260088.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(14681..16816)
                     /gene="pnp"
                     /locus_tag="JN331_001934"
     CDS             complement(14681..16816)
                     /gene="pnp"
                     /locus_tag="JN331_001934"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY7260089.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGCV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(17063..17332)
                     /gene="rpsO"
                     /locus_tag="JN331_001935"
     CDS             complement(17063..17332)
                     /gene="rpsO"
                     /locus_tag="JN331_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY7260090.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(17481..18425)
                     /gene="truB"
                     /locus_tag="JN331_001936"
     CDS             complement(17481..18425)
                     /gene="truB"
                     /locus_tag="JN331_001936"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY7260091.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDSLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LCELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(18425..18826)
                     /gene="rbfA"
                     /locus_tag="JN331_001937"
     CDS             complement(18425..18826)
                     /gene="rbfA"
                     /locus_tag="JN331_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY7260092.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(18990..21662)
                     /gene="infB"
                     /locus_tag="JN331_001938"
     CDS             complement(18990..21662)
                     /gene="infB"
                     /locus_tag="JN331_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY7260093.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNILGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(21687..23174)
                     /gene="nusA"
                     /locus_tag="JN331_001939"
     CDS             complement(21687..23174)
                     /gene="nusA"
                     /locus_tag="JN331_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY7260094.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(23202..23654)
                     /gene="rimP"
                     /locus_tag="JN331_001940"
     CDS             complement(23202..23654)
                     /gene="rimP"
                     /locus_tag="JN331_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY7260095.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         5628 a         6661 c         6001 g         5569 t
ORIGIN      
        1 gttgccagcc cggcacaagc ctcggttagc gatctgaccg gtgatcagcc tgcggcccag
       61 ccggtgcctg taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca
      121 tccgcagagc tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag
      181 gttcagcagg atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag
      241 ttgctgaaga gcaacacccc gctgaacgta ctggcactga accagccgga gaatatcgaa
      301 aaccgcgtca atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg
      361 cgtcatattc gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagtgcattg
      421 ggcgatcgcg tagccaatgc gtttgcgcaa gagtggcaga aactgggcgg cggcaccgtt
      481 ctgcaacaaa aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt
      541 attgctttaa cgggtagccc gattactccc agagcgacaa ccgactccgg catgacgacc
      601 aacaatccaa cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt
      661 gtcgatgcgg tctacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc
      721 gccatgcgta acggtagcca gagcggtgca acgctgtacg ccagttcccg cagcgcacaa
      781 ggcaccgcag gcccggattt ccgactggag atggaaggct tgcagtacag cgaaatcccg
      841 atgctggcag gcggtaatct gccgttaatg cagcaggcgc tcagcgcggt gaacaacgat
      901 tattcgctgg ctcgcatgta tgcgatgggc gttgatgcct ggtcgttggc aaatcatttc
      961 tcacaaatgc gccaggttca gggttttgaa atcaacggta ataccggaag tctgacggct
     1021 aacccggatt gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta
     1081 gtccccgcca gttaaccacc aaacagaccg gcgatgcgtg ggaagcacaa gcgcgtcgct
     1141 ggctggaagg caaaggactg cggtttatcg ccgctaacgt gaacgagcgt ggcggcgaga
     1201 tcgatctgat aatgcgtgaa ggcctgacca ccgtttttgt cgaggtacgc tatcgccgct
     1261 ctgcgcttta tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac
     1321 agactgcccg cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt
     1381 tcgatgtggt agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc
     1441 actcataatt aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa
     1501 agcattcaaa ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc
     1561 atgacgctgg ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact
     1621 tccgctgcca atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg
     1681 cccagcttac ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac
     1741 gatcgcttac atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat
     1801 gtattgttag ccatttccac ccgtggcaac agccgcgata ttgttaaagc agttgaagcc
     1861 gccgttacgc gtgatatgac cattgtggca ttgaccggct atgacggcgg cgaacttgca
     1921 ggtttgttag ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt
     1981 caggaaatgc atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc
     2041 cctcaccagg atgattaagg agaatacatg aaggcattat cgccaatcgc agtccttatt
     2101 tccgcgctgc tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt
     2161 accaaagccg caactgaccc acgcagtgtc ggcacccagg tggacgatgg taccctggaa
     2221 gtgcgcgtga acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat
     2281 gtcacggcct atcagggcaa agtgctgctg gttgggcagt caccaaatgc tgaactttcg
     2341 gctcgcgcca aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt
     2401 cgtcagggcc agccgattgg tctgggcgaa gcatctaacg atacgtggat caccaccaaa
     2461 gtgcgttcgc agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc
     2521 gaaaacggtg aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca
     2581 gatattgcca gccgggtgag cggcgtaaag cgggtaacca cagcgtttac gtttattaaa
     2641 tagcagcccc ttgtaatgcc tgatgcgacg cttgccgcgt cttatcatgc ctacaacgat
     2701 gcaaaccgta ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag
     2761 aacaacagcg ccagcccgcc gacaatcaca ccagacactg ccaccatcgc ccctgtcagc
     2821 cataaggctt tcgccgggaa cgctttgcgc agcataatca gtgacggcaa actcactgcc
     2881 gggagcgtca tcaacaatgc cagcgccgga gcggttccca tacctgccag catcatcgtt
     2941 tgtacaatcg gaatttctgc tgccgtggga atgacaaaca agcatcctgc taccgccatc
     3001 gtcaccaccc acatcaggct gttatcgaca gcaccatcgg catgggggaa taaccagacg
     3061 cgtgcggcac ccaacaccag tactgcaagg atgtaaaccg ggatcgtact ccagaaaagt
     3121 gtccatagcg ccctgcccca gcggctaaaa aaaccgctct gtgcttccgg tatgtcaatt
     3181 tcgaccggtg cctgcgtttg cggtgtttca cgcacccatt tttgcaccag cgtcgcaatc
     3241 agcaacacca tcaccagccc ggccaccaga cgaatcgccg caaaacccca gccgaggaca
     3301 aaccccataa acaccagcgt cgccgggttt aacaccggat tgcccatcca gaatgccagc
     3361 gcaccgccca tcgatacctg ttggcgacgc attcccgccg cgaccggagc cgcacagcag
     3421 gtacacatca tgcccggcag cgaaaacagc gttcccagca gcgtgccgcg aaagcgcgat
     3481 tgcccaagcg tacgcaacaa ccagtcacgc gggatcagca cctgaatcaa cgaaccgagg
     3541 atcaccccca gcaccgccgc tttccatact gcgaggaaat agatcatcgc gtaatccaac
     3601 gccgcttgcc acggattagc atccgcctgc gcaaggatag atttaccgat actgtgggtt
     3661 tcggctgcag taaacgcttt gccgtagtaa ggttgccatt tcacatacca gagaccggca
     3721 atgacaacga gaaagaaaag cgcgggtttc caccactgaa tgggcgttgc cgcctgagat
     3781 gaagactgac cagtcatagc attccccagg aataagttat gataattgag cgcgtgaata
     3841 ttacgctcac tatcaattct tgggaataat tattcagctc ttttgcgtaa ttctgaagag
     3901 cttaaaatcg tcacgccttc atgctcaggc cgcatcgcct ctgccagcat gacccgcgcc
     3961 acgtctctcg catcaatgga tttccagtta cctggtaaca aacggaacag cggcgcaaaa
     4021 agcgtttcgt tcatccgctg tttgctacga tcgcccagta acatcgacgg gcgagcaatg
     4081 gtcaatttcg gccagttctg ggcaattaat gcttcttcca tctccccttt gacgcggtta
     4141 tagaaaaacg gcgagtgggc attggcaccc atagcactga ccaccagcat atgctgcgcg
     4201 cccagtcgcc gcccggttaa tgcggtatcc actaccagcg tgtaatcggc atgaataaac
     4261 gcctctttgc tccccgcttc tcgccgcgtg gtgccgagac aacaaaacac aatgtcgatg
     4321 ggttcggtga cctgcgccaa cgcgtcactc agttgcggat catggggatt aaacaccccg
     4381 ggcatatcgc ccaacggacg tcgcgtcggc gcagcaatgg cgttaacttt cggttcatta
     4441 atcaacatcc gcagcaggtg accgcccacc agccccgttg cgcctgtaat cagtacctga
     4501 ctcatcttcg ctcctttaca gaattgtctg ccttgcgctc cccggctcat gcaccagtct
     4561 taatagacca tgaggtaatt atccccgatt gtggaaaatt cgcctcatcc aatgcaacaa
     4621 cgcggaggaa gcatgagtaa gaaaattgcc gttttaatca ctgatgaatt tgaagattca
     4681 gaatttactt cacccgcaga cgagttccgt aaagccggac acgaagtgat tactattgaa
     4741 aaacaagcgg gtaaaacggt gaaaggcaaa aaaggagaag ccagcgtgac catcgataaa
     4801 tccatcgatg aagtgacgcc agcggagttt gatgccctgt tgctaccggg tggtcattca
     4861 ccggattatc tgcgtggcga caaccgtttt gtcaccttta cccgtgattt tgtgaatagc
     4921 ggcaaacctg ttttcgccat ctgtcacggt ccgcagctgc tgatcagcgc cgatgtgatt
     4981 cgggggcgca aactgaccgc agttaaaccg atcattattg atgttaaaaa tgcgggcgtg
     5041 gaattttacg atcaggaagt cgtggttgat aaagatcagc tggctaccag ccggacaccg
     5101 gacgatctgc cagcgtttaa ccgcgaggcg ttacgcctgc tcggtgcctg agtcgcgtag
     5161 ccaaatcatt tttttaccaa agcccagcgt gttgtcggtg aatttgattt catcgagccg
     5221 gatttcccac accggggccg acagcattct ggcaaccgga aagcgacgat tgtacgcctt
     5281 gcgcgcgagg tcgctttcct caccttccag cctgcggatc tcacctttaa actgcacacc
     5341 gcgaattaac gctaccgttt tcggctgacc gtttaccgtt agcctgcggc ccactcatct
     5401 gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc gactttctgc gcatcaaaaa
     5461 gataaaaggc attagcgcac cagagttccc cttcctgctg cacacaccag gtgacaacat
     5521 gttgttttgc cagccaacgg ctgatggcga tgagtgtttc cattgctgtt ctcttttata
     5581 ctgtgggcct gaccttaaca tgcgatgcag acgatgacac cctggtttct ttacttgatc
     5641 cgtaccgccg acaataagct ttataccggg atcaccacgg atgtcgaacg ccgctatcag
     5701 cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact gacgctggcg
     5761 ttttctgcgc cagtgggcga ccgttcgctg gcactacggg cggaatatcg cgttaagcaa
     5821 ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg cagggtttgc agaactgttg
     5881 agcagcctgc aaaccccgga gattaaaagc gattgaaatg ctcgtgatac tcaaccagac
     5941 cggttacgcc attcagcgcg tcatccgcta aacgatgtac ctggaaggcg ctttcagtgc
     6001 ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc aaagccgaaa cgactgtaca
     6061 gcgccggatc gcccagcgtc accactgcgg catagccgaa ctcattaagc gaatcgagtc
     6121 cttcatagac cagttggcgt gccagcccct gtccacggta tttttcatca accgccaacg
     6181 gtgccatgcc gacccattgc aggtcttcgc cctgcacatc aaccggacta aatgccacat
     6241 agccaatgac ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga aagccatctt
     6301 cacgcagatc gtgaaccagc ttcgcttccg catcactttc gaatgagcga cgcagcaggg
     6361 catcaatacc cggcgcatca atgggaattt ctactcgaat tagcatggtt cacctaccga
     6421 tgtctgtttg gtttcaggcg cggttttcat tcccgcctca acaaaatccg ccagttgcag
     6481 cagcatcatg cgtaaggctt tcggcatttg ctccagttca atggcgtcca tcaggttttt
     6541 cacatacacc cccagctccg tatcgccttc aatcaccagc cgacgctgga agaagagcgt
     6601 atccggatct tgtttacgcg ccgcaatcat cagcagatcg ctggcgtcgg cactaaaact
     6661 cacatcagct tgcgcgttct ggctgaccac cagtttgcca ttcaccaccg aggtaaacca
     6721 ttgcaggtca atatcacgca catgaatact taaccagcgg ccttcaagaa actccagctc
     6781 gccatcatcc agcgcctgac ggaattgcca gcttaagacc tgctcaagaa cctggcgttt
     6841 tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga gatggcccca aatgcacaat
     6901 acgggaacgc agtttatcca acacgagttt tactccctgt ttcaacaatc atcctatttt
     6961 gccatatcag aaaaataaca tagcggtata aatcaacaat tccatatgaa attgctgcta
     7021 ccaccaatac aactttaact gccttaaatc aaaaattgtc gcagcaaggt taactaaaat
     7081 cccagttcgt taacattttt acgttttgat agcgcaacct tcaggaaaaa ttatggagct
     7141 gctctgccct gccggaaatc tcccggcgct taaggcggcc atcgaaaacg gcgcagatgc
     7201 tgtttatatc gggctaaaag atgataccaa tgcccgtcac ttcgccggcc ttaactttac
     7261 cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa catcgccgca aacttcacat
     7321 cgcgattaac acttttgcgc atccggacga ttacgcccgt tggcagcgtg ccgtggatat
     7381 ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc tggagtacgc
     7441 cgccgagcgt tacccgcata ttgaacgtca tgtgtcagtg caggcttcgg cgaccaatga
     7501 agaggcgatt aacttttatc atcgccattt tgacgttgcc cgcgtggtgc tgccgcgcgt
     7561 gttgtcgatt catcaggtga aacagctggc acgggttact cctgtcccgc tggaagtgtt
     7621 tgctttcggc agcctgtgca ttatgtcgga aggccgttgc tatctgtcgt cgtatctgac
     7681 gggtgagtcg cccaacactg tgggtgcgtg ttctccggcc cgtttcgtgc gctggcaaca
     7741 aacgccgcag gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt atcaggacgg
     7801 cgaaaacgca ggttatccga cgctgtgtaa agggcgttat ctggtggacg gcgagcgcta
     7861 tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa ctgctgccgg agttaatggc
     7921 tgcgaatatt gcttcggtga aaattgaagg ccgccagcgt agcccggcgt atgtcagcca
     7981 ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag gccgatccgc aaaacttcgt
     8041 accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc gaaggcacgc aaaccaccct
     8101 tggcgcgtat caccgtaaat ggcagtgaga agagcaatga aatattcctt agggccagtg
     8161 ctgtggtact ggccaaaaga gacgctggaa gatttttatc agcaggccgc cgccagcagc
     8221 gccgacgtga tttatcttgg tgaagcggta tgcagcaagc gccgggcaac caaagttggc
     8281 gactgtctgg agatggcaaa atcgctcgcc ggaagtggca agcagattgt gctctccacg
     8341 ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga aacgctatgt tgagaacggt
     8401 gagtttctga ttgaagccag cgatctcggc gtggtgaata tgtgcgctga acgcaaactg
     8461 ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg tgacgctgaa aatattgctc
     8521 aaacagggca tgatgcgctg gtgtatgccg gtggagcttt cccgcgactg gctggtgaat
     8581 ctgcttaatc agtgcgatga gctgggcatt cgcaaccagt ttgaagtgga agtcctgagc
     8641 tacggtcatc taccgctggc ctactccgcc cgctgcttta ccgcgcgttc ggaagaccgc
     8701 ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga acgggcgcaa cgtgctgtcg
     8761 caggaaaacc aacaagtgtt tgtactcaat ggcattcaga ccatgagcgg ctacgtttac
     8821 aacctcggta acgagctggc atccatgcag ggattggttg atgtagtccg cctgtcaccg
     8881 cagggcactg acactttcgc gatgctcgac gccttccgcg ctaatgaaaa tggcgctgtg
     8941 ccactgccgt tgacggcaaa cagcgactgt aacggctact ggcggcggct ggcaggactg
     9001 gagctgcaag cctaagtaaa tagctcactt tgttaacaac tttaactatt ctttaatgca
     9061 gtattaaaga ttaatcggta acaaagtgag ctgttatgac tgataaaacc attgcgtttt
     9121 cgctactcga tctggccccc atacccgaag gctcttcagc gcgggaagcg ttctcccact
     9181 ctctcgatct cgcccgtctg gctgaaaagc gcggctatca tcgctactgg ctggcagaac
     9241 accacaatat gaccggcatt gccagtgctg caacatcggt attgattggc tatctggcgg
     9301 cgaataccac cacgctgcat ctggggtctg gcggcgtgat gttgcctaac cactcaccgt
     9361 tggtcattgc cgaacagttc ggcacactca atacgctcta tccggggcga atcgatttgg
     9421 ggctgggtcg tgctccgggt agtgaccaac ggacaatgat ggcgctacgt cgtcatatga
     9481 gcggcgatat tgataatttc ccccgcgatg tcgcggagct ggtggactgg tttgacgccc
     9541 gcgatcccaa tccgcatgtg cgcccggtac caggctatgg cgagaaaatc cccgtgtggt
     9601 tgttaggctc cagcctttac agtgcacaac tggcggcgca gcttggtctg ccatttgcgt
     9661 ttgcctcaca cttcgcgccg gatatgttgt tccaggcgct gcatctttat cgcagtaact
     9721 tcaaaccgtc ggctcggctg gaaaaaccat acgcgatggt gtgcatcaat attatcgccg
     9781 ccgacagcaa ccgcgacgct gaatttctgt ttacctcaat gcagcaagcc tttgtgaagc
     9841 tgcgccgtgg cgaaaccggg caactgccgc cgccgattca aaatatggat cagttctggt
     9901 caccgtctga gcagtatggc gtgcagcagg cgctgagtat gtcgttggta ggcgataaag
     9961 cgaaagtgcg tcatggtttg cagtcgatcc tgcgcgaaac cgacgctgat gagattatgg
    10021 ttaacgggca gattttcgac caccaggcgc gcctgcattc gtttgagctg gcgatggatg
    10081 ttaaggaaga gttgttggga tagtgtgtct taacgcggga agccttatcc gagctggcaa
    10141 cgctgtccta cgtaggcctg ataagcgaag cgcatcaggc attgtgtagg cagcagaaat
    10201 gtcggataag gcaccgctga ttactgatac accggcagta aattaaagct cgataaaata
    10261 tgcaccagtg cgttgccgac gccaaacacc agaatcagcg caatcatcgg cttgccaccc
    10321 cagacgcgga atttcgggct gccaaagcgt ttacgcgatg cgcgggccag cagtgccgga
    10381 acaattgccg cccagatggt agccgctaaa ccagcataac caatggcata caggaatccg
    10441 ttcgggaaca gcagaccacc cacaacaggc ggggcaaagg tcagcaatgc cgttttcaag
    10501 cggcccacag ccgagtcgtc gaaaccaaac agatctgcca gatagtcaaa caaacccagc
    10561 gttacgccaa ggaacgaact cgctaccgca aagtttgaga acacgaccag cagaaggtcc
    10621 aggctacggc tgttcagcac gccgcttaat gcttgtacca gcacatcaat attaccgccc
    10681 ttctcggcga tgccgataaa ctccggacga gggatgttgc ccattgtcgc cagcaaccag
    10741 atggtataca gcgccagtgc catcagcgta ccgtacacca ggcatttcac gatggttttc
    10801 ggatctttgc cgtaatactt catcaggctt ggcacgttac cgtgataacc aaacgacgcc
    10861 aggcagaacg gcagggtcat cagcaaatac ggcgcataag atgcattgct ttcggcaacg
    10921 ttgaacaatg tcgcaggctg cacatgcccc agcaggctac cgaaggtgag gaagaaggta
    10981 atgactttcg cccccagcac aatcgccgtc atgcggctga cggctttagt actcaaccac
    11041 accacaaacg ctaccagcag tgcaaaacca aaacccgccg cccgtgccgg gacgtttagc
    11101 gacatctctg cgaaggtgtg atgcagaatc gaaccactgg cagaaatata ggcataggtc
    11161 aggatataga gcacaaaggc aatggaaatg ccgttgacca cgttccagcc tttgcccagc
    11221 aaatctttgg tgatggtgtc aaaactcgaa ccgattcggt aattcaggtt agcttccaga
    11281 atcatcaagc cggaatgcag catacagaac caggtaaaga tcagcgctgc cattgaccag
    11341 aaaaaccacg ccccggacat gaccactggc agagaaaaca tccctgcgcc aataatggtg
    11401 ccgccgataa tcaccacgcc gccaagcagc gacggtgacg tttgggtggt ggttagtgtt
    11461 gccatgaggg cttctctcca gtgaaaaata gtgcgactgc gttgttatgc attgcactgt
    11521 accagtacat gagtacaaaa gacagaaaaa aagccccgat ggttaaaatc ggggctgtat
    11581 atattatttt acagattgtg ttcgctgttc agcgatgatt acgcatcacc accgaaacga
    11641 cgacgaccgg tagagtcatc acgacgcggt gcgcggcctt cacgacgttc gccgctaaaa
    11701 cgacgaccat caccacgacc accttcacgg cattcaccgc tgaagttacg accgccttca
    11761 cgacgctcgc caccgaaacc acgaccaccg ccacgacgct caccgccagt atgcggctgt
    11821 gcatcgccca gtaactgcat gttcatcggc ttgttgagaa tgcgagtgcg cgtaaagtgt
    11881 tgcagcactt cacccggcat acctttcggc agttcgatgg tggagtgaga agcaaacagc
    11941 ttgatgttac cgatgtaacg gctgctgatg tcgccttcgt tagcaatcgc gccaacgata
    12001 tgacgaactt caacaccatc atcgcggccc acttcaatgc ggtacagctg catatcgcca
    12061 acatcacgac gttcacgacg cggacgatct tcacggtcgc cacgcgggcc acggtcgtta
    12121 cgatcgcgcg gaccacggtc atcacggtca cggaattcac gtttcggacg catcggcgca
    12181 tctggcggaa caatcagagt acgttcaccc tgtgccattt tcagcagtgc cgcagccaga
    12241 gtttcgagat ccagctcttc accttctgca gtcggctgaa ttttgctcag cagtgcgcgg
    12301 tattgatcca gatcgctgct ttccagctgc tgctgtactt tagcggcgaa tttttccaga
    12361 cggcgtttgc ccagcagttc tgcgttcggc agttctactt ccggaatagt cagcttcata
    12421 gtacgttcaa tgttgcgcag cagacgacgc tcgcggttct caacgaacag cagcgcgcgg
    12481 ccagcacgac ccgcacgacc ggtacgaccg atacggtgaa cgtaagactc agaatccatc
    12541 gggatatcgt agttaaccac caggctgata cgctcaacgt ccaggccacg ggccgcaacg
    12601 tcggtcgcaa tcaggatgtc cagacgaccg tctttaaggc gttccagtgt ctgctcacgc
    12661 agcgcctggt tcatgtcacc gttcagcgcg gcgctattgt agccgttacg ctcaagagct
    12721 tcagccactt ccagagtcgc gtttttggta cgaacgaaga taatcgccgc atcaaaatct
    12781 tccgcttcca ggaaacgtac cagtgcttcg tttttacgca taccccacac agtccagtag
    12841 ctctggctga tgtcaggacg ggtagtcacg ctggactgaa tacgcacttc ctgcggctct
    12901 ttcataaagc ggcgggtaat gcgacgaatc gcttccggca tggttgcaga gaacagagcg
    12961 gtctgatgac cttccgggat ctgcgccata atggtttcaa cgtcttcgat gaagcccatg
    13021 cgcagcattt cgtcagcttc atccagaacc agaccgctca gtttagagag gtccagagtg
    13081 ccacgtttca ggtggtccag cagacggccc ggagtaccga caacgatctg cggcccctga
    13141 cgcagggcgc gtaattgcac gtcataacgc tggccgccgt acagggcaac cacatttacg
    13201 ccgcgcatgt gtttagagaa atccgtcatt gcttcagcaa cctgtaccgc cagttcgcgg
    13261 gtcggtgcca gcaccagaat ctgtggtgct ttcagctcag gatcaagatt ctgcagcaga
    13321 ggtaaagaga atgctgcagt ttttccgctc cccgtctggg ccatacccag aacgtcgcgg
    13381 ccattcagca gatgtggaat acactctgcc tgaattggag atggtttttc gtaacccaga
    13441 tcgttaaggg cttcaaggat aggagccttc aggcccagat ctgcaaaagt ggtttcgaat
    13501 tcagccatgt agtacgtgtg cctcaaaatt aatggcggcc agtctacata actcatcatg
    13561 aaattgatca gcaattttca ttgaaaagtg tgaaccggct caaagtaggt gtattaacga
    13621 acaacaacgc cctcacccgt taaggtgatg gcaatcaaaa aagattacgg gctgatgtgt
    13681 acgtcagcta ttgctggtcc gattctgcca ggtcatcttg gtcctggccc aggagcgata
    13741 attccaacaa tgcgtatcgg tgctcaacaa agttatgaac gttgttggca accgccagtt
    13801 tgaacagtgc cgtggcgctg tccaaatccc ccagacttag gtagtactta cctaaataga
    13861 agttggtttc actgagatgc tcagcgagcg aggtgttatc cgttgcgtcc gccttgagcc
    13921 tttccattaa cgtttgttcg ctaatgttgc ccaggtagaa ctcgacaatg ttccatcccc
    13981 actgttcctt atccgatttt tcgaagtgct gtttcaacac ttctttagcc tgcttctcat
    14041 cgagcttctg ctcggcgaga taaagccaca gactacggaa aggatcattg ggatcgtctt
    14101 gataaaacgc cagcagatca tcttgcgcta acttgtcacg accgccgtaa tataatgcga
    14161 tcccgcgatt caagtgcgcg tagttgtaag ttggatcaag ctcaagtaca gaatcaaacg
    14221 cttcataggc agcatcaaaa ttgcctgcct gcgttaaata tatgcctaag taattgaata
    14281 cttcaggcat atccggtcgg attgccagcg cttgcgaaaa atcgttacgc gctaatgccc
    14341 tcagaccgag actatcatac aacactccgc gctcatataa aagctgtgcg cgttcgtcat
    14401 cggttaaagc ccgactggca aggatttgtt ccatacgtgc cagaatcact tcctgctgta
    14461 aagtcggttg caatggtacc gcgaggactt cacttttacg ccaggaagta ttactgcatc
    14521 ctgcaagcgt aagtgctgtc gcaacgaaac accagcgcaa aaaaggcttc atttcccact
    14581 cccgaagacc acggttgaat gaacgtcctg ttcccggttg ctaacaaggc gtcctgcccg
    14641 gttaaaagcc ccccgccgca gcggagggca aatggcaacc ttactcgccc tgttcagcag
    14701 ccggagcttc cggtgctgca gcaggttgag actgctcagt cgcttcttta atgctcagac
    14761 ggatacggcc ctggcgatca acttccagaa ctttcaccgg tacttcctga cccatctgca
    14821 ggtaatcggt cactttctca acgcgtttgt cagcgatttg agagatgtgt accagacctt
    14881 ctttaccgcc gccgatggca acaaatgcgc caaagtcaac gatacgggtc actttaccag
    14941 tgtagacgca gcccacttcg atttctgcag tgatctcttc gatacgacga atagcatgtt
    15001 tcgctttctc gccgtcggtc gctgcaatct tcacagtacc gtcatcttcg atttcgatgg
    15061 tggtgccagt ttcttcggtc agggcacgga taacagagcc gcctttaccg ataacgtctt
    15121 tgatcttgtc cgggttgatc ttgatggtat ggatacgcgg tgcgaactca gagatatcgc
    15181 cacgcggcgc gttgatcgcc tgttccatta cgcccaggat atgcagacgc gcacctttag
    15241 cctggttcag cgcaacctgc atgatctctt tggtgatacc ttcaattttg atatccatct
    15301 gcaatgcaga gataccctcg cgggaacccg caactttgaa gtccatatcg cccaggtgat
    15361 cttcgtcgcc caaaatgtca gacagtacaa cgtagttgtc gccttctttc accagaccca
    15421 ttgcgatacc cgcaacggca gctttgatcg gcacacctgc gtccatcagc gccagagacg
    15481 cgccgcacac ggaagccata gaagaggaac cgttggattc agtgatttca gacacaacac
    15541 gtacggtgta cgggaatttg tccatatccg gcatgactgc cagcacgccg cgcttcgcca
    15601 gacgaccgtg accaatttca cgacgcttcg gagaaccgac catgccggtt tcgcctacgg
    15661 agtacggagg gaagttgtag tggaacagga aggtatcggt acgttcgccc atcagttcat
    15721 caagaacctg cgcgtcacga gcagtaccca gcgttgcggt aaccagcgcc tgcgtttcac
    15781 cacgggtgaa cagcgcagaa ccgtgagtac gcggcagcac gccagtacgc acatccagac
    15841 cacggatcat atctttttca cgaccgtcga tacgcggttc gcctgccagt acgcggctac
    15901 gaacaacgtt tttctcgatc gcgtgcagaa tttcacccag ttcgttttcg tccagggttt
    15961 cgtcttcagc aagcagcgtc gcgatggttt cagatttgat gacatcaacc tgcgcataac
    16021 gctcttgttt gtcggtgatg cggtaagcat cgctcaggcg agcttcagcc agtgcagcaa
    16081 cgcgcgcgtt cagcgcttcg tttaccggct ccggctgcca gtcccaacgt ggtttaccgg
    16141 cttctttcac cagttcattg atgttctgaa taacaacctg ctgttgttca tgaccgaaca
    16201 ctactgcgcc cagcatctgg tcttcgctca gcagttcagc ttcagattca accatcagta
    16261 cagctgcttc agtaccggca acaaccagat ccagtttgct ctctttcagc tcgtcctgag
    16321 tcgggttcag tacgtactgg tcattgatgt aacctacgcg agcagcacca atcggaccat
    16381 tgaacggaat accagacaga gacagtgctg cggaagcacc aatcatcgcg acgatatccg
    16441 ggttaacttg cgggttaaca gaaaccacgg tggcgataac ctgaacttcg ttgacgaagc
    16501 cttccgggaa cagcgggcga atcgggcggt caatcagacg cgcgatcagg gtttcgcctt
    16561 cgcttgggcg gccttcacga cggaagaagc tacccgggat acgaccagca gcgtaggtac
    16621 gctcctgata gttaacggtc agtgggaaga agtcctgacc tggtttggct tttttctggc
    16681 caacaacggt aacaaatacc gcggtgtcat ccatgctaac cataacagcg gcagtagcct
    16741 gacgagccat catgccggtt tccagagtca cggtgtgttg gccgtactgg aatttacgaa
    16801 cgatcggatt aagcaatgta atatcctttc tctttcttag acagtacctt acggcactgg
    16861 tgttaatacc cgatcttctg cgcatcctcg cgactaatga caaccctaac ccagctctat
    16921 gtgggtaaag cctctcatta gccgcgcgaa cctctgcaac ggaagatcat tcatagcaac
    16981 aatacattag tttccagtga attgctgccg tcagcttgaa aaaaggggcc actcaggccc
    17041 ccttttctga aactcgcaag aattagcgac gcagacccag gcgctcgatg agctgggtgt
    17101 aacgtgctac gtctttacgt ttcaggtagt cgagcagttt acgacgctga gaaaccatgc
    17161 gcagcagacc acgacggctg tggtgatctt ttttgtgctc tgcaaagtgg ccctgcaggt
    17221 ggttgatctg tgcagtcagc agtgctacct gaacttcagt agaaccggtg tcgtttgcgt
    17281 cacgaccaaa ctcagaaacg attttagctg ttgcttcagt acttagagac attttaaaac
    17341 tccaaagtat atagaatgaa aggacgccga tctctaattc agcgatccca gtgtacgtta
    17401 cgcaaagtgt taaacaattt acgcgacgtt aagcggcagt attctactcg tagcgacctg
    17461 ttatcgcaag acggttaaca ttacgccggg tattcaacca ccaggcgacg aggcgcaacg
    17521 cggccttcat cgtcaatttc gcccataccg ataaatttgc cgttctcacc ttctgtaacg
    17581 cgaaccagtc cttccagcgg cgcaccggat gtacgaactg ggttaccatt tttgaagtaa
    17641 acagaagacg ttaacggaag attcaccacc gggtagtccg aagctggact gtccattggc
    17701 atcagtaatg gatcaagtaa ctccgcagcc ggaatatcct gctgttcggc ttgttcgacc
    17761 agttcacaca gatgttccag ggtcaccatc cgttcaaccg gatatttact taccgccaga
    17821 cgacgcaggt aaataacatg cgcgccacaa ccgagttttt caccaaggtc atcaatgatg
    17881 gtgcggatat aagtgccttt tgagcagtga atttccagct ccagctcatt gccttcgtgg
    17941 cgaataaaca gcaattcata aacggtaatc gggcgcgctt cacgcggtac ttcaatgccc
    18001 tgacgcgcat attcgtacag ttttttaccc tgatatttca gtgctgaata catcgaaggg
    18061 atctgttcga tatcgccacg gaaagtatcc agcgccgccg ccagttgctc tgcgctaaag
    18121 gttaccggac gttcttcaac gatttgtccg tcggcatcag aagtatcggt acgctgtcca
    18181 agacgcgcaa tgacccgata gcgtttgtcg gagtccagca gatactggga aaacttcgtc
    18241 gcttccccga ggcaaattgg caacatgcca gttgccagcg agtccagcgc accggtatgc
    18301 ccggcacggt tggcgttata aatccgtttc actttttgca gcgcatcatt gctggacata
    18361 ccctgcggtt tatccagcaa caaaacgccg ttaatgtcgc gaccgcgacg acgaggacga
    18421 ctcattagtc ctccttgctg tcgtccgggt taacacgacg ttcttcgtca tgtttgacca
    18481 cgctggtcac caggtttgac atgcgcatcc cttcaaccag agagttgtcg tagaagaagg
    18541 tcagttccgg cacgatacgc aggcgcatcg ctttccccag caggctgcgg atgaaaccag
    18601 aagcttcttg caacgctttg atgcccgctt taaccgcgtc ttcatctttg tcgttgagga
    18661 acgtcacata tactttggca tacgccaggt cgcgagacat ttcgacaccg gaaacggtgg
    18721 tcatcatgcc caggcgagga tctttaattt cacgctgcag gatgagagcg atctcttttt
    18781 gcatttcctg cgctacgcgc tgcgggcgac caaattcttt cgccataata aattctcctg
    18841 acaaaaaagg ggctgttagc ccctttttaa aattaatttc aggtggaagg gctgttcacg
    18901 ttggcctgat aagacgcgcc agcgtcacat caggcaatcc atgccggatg cggcgtaaac
    18961 gccttatccc gcatggaacc ctaaaaacct taagcgatgg tacgttggat ctcgatgatt
    19021 tcgaatactt cgatcacatc gccagtgcgg acgtcgttgt agttcttaac gccgatacca
    19081 cattccatac cgttacggac ttcgttaacg tcatctttga agcggcgcag ggactccagc
    19141 tcgccttcgt agataaccac gttgtcacgc agaacgcgga tcgggttgtg acgtttaacc
    19201 acaccttcgg taaccataca gcctgcgatg gcaccaaatt tcggcgattt gaacacgtca
    19261 cgaacttccg ccagaccgat aatctgctgt ttcagttccg gagacagcat accgctcatc
    19321 gccgctttca cttcgtcaat caggttatag atgacggagt agtaacgcag atccaggctt
    19381 tccgcttcaa tcactttacg tgcagaggca tcagcacgta cgttgaagcc aaccaggatg
    19441 gcgttggacg ccgcagccag ggtggcgtcg gtttcggtga taccacctac gccagaaccg
    19501 atgatcttca ctttaacttc gtcagtagac agtttcagca aggagtcgga gatcgcttcc
    19561 acagaaccct gtacgtctgc cttcaggacg atgttcactt cgtgaacttc gccttcggtc
    19621 atgttggcga acatgttctc gagtttagat ttctgctgac gcgccagttt aacttcgcgg
    19681 aatttaccct gacgatacag tgcaacttca cgcgctttct tctcgtcacg tacaacggta
    19741 acttcatcac ccgcagccgg tacgccggac aggccgagga tttccaccgg aatggacgga
    19801 cccgcttcca gcacttcctg acccagttcg ttacgcatcg cacgaacacg accgtattcg
    19861 aagccacaca gaacgatatc gcccttgtgc agagtacctt cacgtaccag aacggtagca
    19921 accggaccac gacctttatc gaggaaggat tcgataaccg caccgctcgc cataccttta
    19981 cgtaccgctt tcagctccag aacttccgcc tgcagcagga tagcgtccag cagttcatcg
    20041 ataccagtac ccgctttcgc agacacatgt acgaactggc tttcaccgcc ccactcttcc
    20101 ggcaggatgc cgtactggga gagttcgttc ttaacgcgat ccggatcggc ttctggttta
    20161 tcgatcttgt tcactgcaac gaccaccgga acctgcgccg ctttcgcgtg ctggattgct
    20221 tcgatcgtct gcggcattac gccgtcgtcg gcagcaacaa ccaggactac gatgtccgtt
    20281 gcctgcgcac cacgagcacg cattgaagta aacgcggcgt gccccggggt gtccaggaag
    20341 gtgatcatgc cgttttcagt ttcaacgtgg tatgcaccaa tgtgctgggt aatgccgccc
    20401 gcttcgccag aggccacttt cgttgaacga atgtagtcca gcagagaggt tttaccgtgg
    20461 tcaacgtgac ccatgatggt cacaaccggc gcgcgcggtt cagccgcagc acccgtgtca
    20521 cggtcgctca ttaccgcctc ttccagctcg ttttcacgac gcaggataac tttatggccc
    20581 atctcttcag caaccagctg tgcggtttcc tgatcgataa cctggttgat ggttgccatt
    20641 gcgcccagtt tcatcatcgc tttgatgacc tgagagcctt taaccgccat cttgttcgcc
    20701 agttcgccaa cggtgatagt ttcgccgatc acaacgtcac ggttaacggc ctgagcaggc
    20761 ttctggaagc cttgctgcag cgaagaacct ttacgttttc cgcctttacc gccacgtact
    20821 gctgcgcgtg cttcttcacg atcagctttt gattcagcgt gtttgttgcc tttcttcgga
    20881 cgcgctgctt tcgcgttacg accacggcca cggccgcctt cgacttcacg atcgctttcg
    20941 tcttctgcct ggcgagcatg ttgagaagta gtgacgtgat aatcgctgga atcttcagtc
    21001 ggttccgcgt tatcagtcca tttgttttct tccgccatac gacgtgcttc ttcagcaacg
    21061 cgacgtgctt cttcttcgag tttacgacgc gcttcttctt cagctttacg cttgagctct
    21121 gcagcttcct gctcacggcg ggctttttca gcctgggcgt ttttagtcat atcgtcttgt
    21181 tgattgctca ctttgtcttt ttccgcagct tcacgtttcg cttgttcagc agcttcacgc
    21241 ttagcttgtt ctgcggcctc acgttcagct ttttgttgcg cctcgcgttt agccgattct
    21301 tctgcctcac gacgggcttg ctcttccgct tcacgctgcg cttgctcttc cgctgcaagg
    21361 cgttcagcct cttgcggatc gcgtttcaca aaggtgcgtt tcttgcggac ttcgatttgt
    21421 accgatttgc tttttccacc ggtaccaaga atgttaaggg tgctacgtgt tttacgttgc
    21481 agcgtcaatt tgtccgggcc tgaatttttc tgattcaggt ggtcaatcaa agtctgtttc
    21541 tcttgtgcag acacagagtc gtcagcagac ttccggatac ctgcatcagc aaattgctgt
    21601 accaggcgtt ccacggaggt ctgtcgctct gcggccagcg ttttaatcgt tacatctgtc
    21661 atgctgttcc ttcctgctac agtttattac gcttcgtcac cgaaccagca aatattacgg
    21721 gcagccataa tcagtgctcc ggctttttcg tcggtcaacc cttcgatatc agccagatca
    21781 tcaatgccct gttcggcgag atcttccagc gtacaaacgc cacgggcggc cagtttgaat
    21841 gccaaatcac gatctacccc ttcaaggttc agcagatcgt cagccggttt gttatcaccg
    21901 aggctttctt cctgggcctg tgcaatggtg gccagtgcat ttttagcacg ctcgcgcagt
    21961 gcttcaacgg tcggctcatc aaggccttcg atttccaaca gctctttcat cggcacatag
    22021 gccagttctt ccagcgtcga gaaaccttct tctaccagaa cagtcgcgaa gtcttcgtcg
    22081 atgtcgagat atttggtgaa ggtgtcgatc gctgcgtgcg cttccgcctg atgcttagcc
    22141 tgcaggtcgt caacggtcat tacgttgagt tcccaaccgc tcagctgcga agccagacgc
    22201 acgttctgac cgttacggcc aatcgcctgc gccaggttac cggcttcaac ggcgatatcc
    22261 atggtgtgtt tatcttcatc caccacgata gaagcaacgt ctgccggtgc cattgcgtta
    22321 atcacaaact gcgccgggtt atcatcccac agaacgatat cgatacgctc gccgcccagt
    22381 tcagtagaaa ccgcctgaac acgcgcgcca cgcataccta cgcaagcacc taccggatcg
    22441 atacgtttat cgttggtttt caccgcgatt ttcgcacgag aacccgggtc gcgagccgct
    22501 gctttaattt caatcacttc ttcgccgatt tccggcactt caatacggaa cagttcgatc
    22561 agcatttccg gcttggaacg agtgacgaac agttgcgcgc cacgcgcttc cgggcgaacg
    22621 gaataaagca cgccacgaac gcggtcgcca gggcggaagt tttcacgcgg cagcatatct
    22681 tcgcgcagga tcacggcttc agcgttgttg cccagatcca gagagatgtt gtcgcggttt
    22741 acttttttca ccacgccggt gatgatttca ccttcgtgtt cacggaactg atcaaccacc
    22801 atcgcacgtt cggcttcacg cactttctgc acgataacct gttttgccgt ctgggtggtg
    22861 atacggtcaa aggtaacaga ctcaatctga tcttcaacgt aatcgcccag gttcaggctt
    22921 tcatcttcat aacgtgcggc ttcaagggtg atttccttgg tcggctgggt gacttcatca
    22981 acaactaacc agcgacggaa ggtgtcaaaa tcaccgcttt tgcgatcgat ctgtacacgg
    23041 acgtcgatct cttgctcata ttttttcttt gttgctgtcg ccagcgcgct ttccaatgct
    23101 tcgaaaatct tctcgcgagg tagcgccttt tcattggata cggcttcaac tacagccaaa
    23161 atttctttgt tcatcgcggg cttttcacct catccagact attaaaagtg gggaaccagg
    23221 ttcgccttct ggatattact cagcgcgaac acttcatctt taccttcgac ggtaactgtg
    23281 atcatttcac cgtctaccgc tttgataacg ccctgccatt tacgacggtt ttgtaccgcc
    23341 atacggagaa ccagagtcac ctcttctccg acaaaacggg cgtagtgttc agccgtgaac
    23401 agtgggcgat cgagacccgg tgaggagact tccaggttat aagcaacggt gatgggatct
    23461 tcaacatcca gcacagcact tacctggtgg ctcacatcag cacaatcatc aacattgatg
    23521 ccatcttcac tatcaatata gatgcgcagt gtggatgtgc gaccgcgaat aaattcgatg
    23581 cctaccagtt caaaacccag ggcctcaact ggcgcagtaa tcatctctgt taatttttgc
    23641 tctaatgtgg acaagcccac ccccaagaca taaaaaaagg gcctaaagcc cagttattct
    23701 gtagtcagat aacaaaaaac cccgataaat cggggcttta tataactgaa ccctataacc
    23761 gcaactgcgg tctggagcac tttccagaag gattttttca aatcccacta cgaaggccga
    23821 agtcttcaca gtatatttga aaaaggactc taagggaaa
//
LOCUS       DADLSB010000050        23087 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.050, whole genome shotgun sequence.
ACCESSION   DADLSB010000050 DADLSB010000000
VERSION     DADLSB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.050"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(260..877)
                     /gene="tdk"
                     /locus_tag="JN331_001941"
     CDS             complement(260..877)
                     /gene="tdk"
                     /locus_tag="JN331_001941"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY7260096.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1482..1895
                     /gene="hns"
                     /locus_tag="JN331_001942"
     CDS             1482..1895
                     /gene="hns"
                     /locus_tag="JN331_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY7260097.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2039..2947)
                     /gene="galU"
                     /locus_tag="JN331_001943"
     CDS             complement(2039..2947)
                     /gene="galU"
                     /locus_tag="JN331_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY7260098.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3149..4162)
                     /gene="rssB"
                     /locus_tag="JN331_001944"
     CDS             complement(3149..4162)
                     /gene="rssB"
                     /locus_tag="JN331_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY7260099.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4254..5159)
                     /gene="rssA"
                     /locus_tag="JN331_001945"
     CDS             complement(4254..5159)
                     /gene="rssA"
                     /locus_tag="JN331_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY7260100.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5272..5730
                     /locus_tag="JN331_001946"
     CDS             5272..5730
                     /locus_tag="JN331_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY7260101.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5780..6622
                     /gene="purU"
                     /locus_tag="JN331_001947"
     CDS             5780..6622
                     /gene="purU"
                     /locus_tag="JN331_001947"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY7260102.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6782..6866
                     /locus_tag="JN331_001948"
     tRNA            6782..6866
                     /locus_tag="JN331_001948"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6816..6818,aa:Tyr,seq:gta)
     gene            6913..7044
                     /locus_tag="JN331_001949"
     ncRNA           6913..7044
                     /ncRNA_class="other"
                     /locus_tag="JN331_001949"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7228..7905)
                     /gene="narI"
                     /locus_tag="JN331_001950"
     CDS             complement(7228..7905)
                     /gene="narI"
                     /locus_tag="JN331_001950"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY7260103.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7905..8615)
                     /gene="narJ"
                     /locus_tag="JN331_001951"
     CDS             complement(7905..8615)
                     /gene="narJ"
                     /locus_tag="JN331_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY7260104.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDVVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8612..10150)
                     /gene="narH"
                     /locus_tag="JN331_001952"
     CDS             complement(8612..10150)
                     /gene="narH"
                     /locus_tag="JN331_001952"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7260105.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10147..13890)
                     /locus_tag="JN331_001953"
     CDS             complement(10147..13890)
                     /locus_tag="JN331_001953"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7260106.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPIADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNSVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYVH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14283..15674)
                     /gene="narK"
                     /locus_tag="JN331_001954"
     CDS             complement(14283..15674)
                     /gene="narK"
                     /locus_tag="JN331_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY7260107.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16013..17809
                     /gene="narX"
                     /locus_tag="JN331_001955"
     CDS             16013..17809
                     /gene="narX"
                     /locus_tag="JN331_001955"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY7260108.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17802..18452
                     /gene="narL"
                     /locus_tag="JN331_001956"
     CDS             17802..18452
                     /gene="narL"
                     /locus_tag="JN331_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY7260109.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18453..19847)
                     /gene="ychO"
                     /locus_tag="JN331_001957"
     CDS             complement(18453..19847)
                     /gene="ychO"
                     /locus_tag="JN331_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY7260110.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20032..20385
                     /gene="ychN"
                     /locus_tag="JN331_001958"
     CDS             20032..20385
                     /gene="ychN"
                     /locus_tag="JN331_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY7260111.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20429..21124)
                     /locus_tag="JN331_001959"
     CDS             complement(20429..21124)
                     /locus_tag="JN331_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7260112.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21282..21512)
                     /gene="chaB"
                     /locus_tag="JN331_001960"
     CDS             complement(21282..21512)
                     /gene="chaB"
                     /locus_tag="JN331_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY7260113.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21782..22882
                     /gene="chaA"
                     /locus_tag="JN331_001961"
     CDS             21782..22882
                     /gene="chaA"
                     /locus_tag="JN331_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY7260114.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYSVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5784 a         6024 c         5552 g         5727 t
ORIGIN      
        1 ccttctgggg ccgttttttt gtttacccaa agcaacccga cagcattagc cttcatcata
       61 tttgcgacga tgtataacgc ctaaacacag ggatattgta ctttacaggt cacaagtcaa
      121 cgtcggtgct taagagccct gtgaggcgta tagcggcgtt aaaaaactgc cgagaagggt
      181 atatagcccg gaagaagtgc gtaaaacgaa ctgacaggat aaaagtgccc tgctcaccct
      241 gtcagtaaag aaattcttat taatcgtggc gatgcctttc ctgaatagcc gttaatgagc
      301 cgacttgtaa cgcctcttta tagtgtttac ggcatacaga aacgtatcgt tcattaccac
      361 caattaccac ctgctcacct tcgttataag gtctgcctgc ttgatcaaga cgcagcacca
      421 tgcttgcttt acggccacaa aaacagatgg tttttaattc aaccagtttg tcggaccatg
      481 ccagtaagta ttggctgcca ataaataatt cacctcgaaa atcggtacgt aaaccataac
      541 aaagtacggg tatatcgagt tgatcgacaa cctccgataa ttcatatact tgttgtctgg
      601 ttaaaaactg gcattcatca accagtacgc aatgaattgc ctgctgttca tgttccgcac
      661 gaatctcatc aaataatgat gaattttggt taaataattt tgcaggcgat gacaaaccta
      721 tacgcgaact gactttcccg gcaccaaagc gatcatcaat ttctgccgta tatacgacag
      781 tgcgcatgcc gcgttcctgg taattgtatg aagattgcaa caatgctgta gacttacccg
      841 cattcattgc ggaatagtag aaatatagct gtgccatcag ccacaggccc tcaatgatat
      901 gaaataaagt tggctggagt ttatcataat tcgtcagttt ttcagctcaa cagtatgcac
      961 agaagtgggt atatgcgttc tcccttacga agccttgcat aatccttctg aactatcatt
     1021 acaactgcct cgcttgttat aagcgggtaa atgactgctg gtaactattc acaatcttta
     1081 acctgttgcg caagtaatag ccctctgttg acctccaaga gatagtgcaa tactaagtcc
     1141 atgctcttat tgcgactgtt ctacttttca tcattcgctt aatagggaat tctcgtaaac
     1201 acaactaata cagaagactg aaaggtcgtc agcctacgat aatctcccca taaaatgtga
     1261 catgaatcag gaagttttaa cctcacgtgc tgcgaaatca tcggtgtaaa tagggctata
     1321 tgccgcgtct tttctggcta attttatgaa aagatattta ttggcggcac aaaataaaga
     1381 acaattttga attccttaca ttcctggcta ttgcacaact gaatttaagg ctctattatt
     1441 acctcaacaa accaccccaa tataagtttg agattactac aatgagcgaa gcacttaaaa
     1501 ttctgaacaa catccgtact cttcgtgcgc aggcaagaga atgtacactt gaaacgctgg
     1561 aagaaatgct ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa gaaagcgcgg
     1621 ctgctgctga agttgaagag cgcactcgta agctgcagca atatcgcgaa atgctgatcg
     1681 ctgacggtat tgacccgaac gaactgctga atagccttgc tgccgttaaa tctggcacca
     1741 aagctaaacg tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac ggcgaaacta
     1801 aaacctggac tggccaaggc cgtactccag ctgtaatcaa aaaagcaatg gatgagcaag
     1861 gtaaatccct cgacgatttc ctgatcaagc aataatcttt tgtagattgc acttgcttaa
     1921 aatcccgcca gcggcgggat tttttattgt ccggtttaag acaatttaat aagaataagc
     1981 tataaaaaaa cggcgtcgat tgctcaacgc cgtttcgtgg ataacaccga tacggatgtt
     2041 acttcttaat gcccatctct tcttcaagcc aggctttaaa ttccgtgcca agggtgttat
     2101 gacgaatacc gtattcaacg aaggcctgca tgtaacctaa tttattaccg cagtcatggc
     2161 tcttcccttt catatgatag gcttccaccg tttctttttc gatcagcata tcaattgcgt
     2221 cggtgagctg aatttcatca ccagctcccg gaggggtttt tgccagcaac ggccaaatat
     2281 ccgcgctaag tacgtaacga cccacaatag cgagattaga cggcgcaaca tccgctttcg
     2341 gtttttctac cacaccaacc atcggtacgc tttcacccgg cgctaattca acgcctttgc
     2401 aatccacaac gccatatgcg gtcacatcag caaccggttc aaccatgatc tggctatgac
     2461 ccgtttcatc aaagcggcgg atcatctctg ctaggttatc ctgtgacaaa tcggattcat
     2521 attcatccag aataacatca ggcaaaataa cagctaccgg ttcatcaccc actaccgggt
     2581 gagcacacaa taccgcgtgt cccaggcctt tcgccagacc ctgacgaact tgcataatag
     2641 tcacgtgcgg tggacaaata gactgcactt catcaagcag ttgacgtttt acacgttttt
     2701 ccagcattgc ttccagttca aaactggtat caaagtggtt ttcaatagag tttttagatg
     2761 agtgtgtaac cagcacaatt tcagtaatgc cagccgcaat acattcattc acgacgtatt
     2821 gaattaatgg cttatcgaca agtggcagca tctctttcgg gatggctttc gtcgccggca
     2881 acatcctggt tcctaatccc gcaacgggga taacggcttt tttgactttc gtattaatgg
     2941 cagccattta aattctcctg gactgttcgt gttttgaacg tgttcatatt tctgtatcgc
     3001 atcccagtat atcagcaccc cgaaggggct aagttctgaa aaggaggcga gatagcataa
     3061 ttaagacaat tacttatacg aatggcgacg atagtaccac cagccgacat tagcaggtaa
     3121 tgcaaattta gcccgcgtta tcgtttgctc attctgcaga caacatcaag cgcagtcgac
     3181 caccggttcc ccatatttgg cattgccagg catcgcatcg ctggctcaat tgattcaaat
     3241 aagcgttacc taaagtgcct aacggaacac cattactgat ttgcacctgg tgttcgccgg
     3301 tatttaacgt cgcattcaga cccgcagaaa ccagaatgag atttttcagt tcgcgatgat
     3361 aatagccaac taatagcgga aactgccccg gcagattggc ctgacgaagt aaatggttta
     3421 cctgcttcaa taacgcgccc aactctggca accgttgatt ttggtgtgca agctgttcct
     3481 gtaataatcc gttaaacaat gcgcgtaata acaaggcagc aagtacgcca ttatgtccag
     3541 ctcgggtgac atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg gcaatatcaa
     3601 gcaccaggcc gggtttgtcc gcggcaacca attgacggta attaacccgg caatgggaaa
     3661 tcacctgctg aaccggcggt tgtagttcct gtaataattt cgccgctgcg gcagggttat
     3721 caaccattgc atcccagtcg cgaaaaagcc tttcctcttc ctcaacgcgc gaattaaaca
     3781 tgctgggata gagacaggca aaaaccatct cgcgcaagcg attcagatct ttaactggtt
     3841 tcagcaaaac atcttcaacg cccagacgta acgctttggc aatatctgcc atattttcag
     3901 tggcagatat caccagaact ggggtctggt cgcctctgtt acgtatatgc tccagcagtt
     3961 taagcccgtt cattcgtggc atcgcgatat cacatatcat caggtctgga gtgaaacctc
     4021 ccagcaactc aagggcatcc accccatcag ccgccagtac cgttgtcgct cccaatgagg
     4081 aaaaccatga atccagaagc gagcgaaata cctgctcatc ttcaacaatg agaatctgtt
     4141 ttccgaccaa tggctgcgtc atgttctctc ccctgactgg ctttactcaa tagtggcatg
     4201 ctattgccac ctgcgcctgt cagaattaac ttaagtgtag ataaaaattc tggtcaaatg
     4261 ttggtgcgta ccaacggcaa aagttcgtcc attttccttt ccactgccag ctgtccggcc
     4321 gcaatggcag cgtgcgcgcg atggaaatca agcgtagata tttgcgggca aacaggttga
     4381 atcagaatat cgggcggatc acctgccatg cggttccttt taaggcggtt ctccaacacc
     4441 tggatagacg tggtcataat ctctgttgcc gttggcgctg tcacagcccg acgtgtcgtt
     4501 atgctgccta acctttcttt cagacgcgca tgccacggta gagaatcacc attctcgcta
     4561 ttttcttcac tgacattaaa ggagagcaga tcttgttgca tcaaatgagc atcgtgctgc
     4621 aggtcgaccg ctatcacaat atcagccccc aatgcacgcg taagagaaat aggaattggg
     4681 ttaactactg ccccatcaac cagccagtag ccgttatgtg caacgggtgc catgagtcct
     4741 ggaatactgc atgatgcgcg aatagcaaga tggagatcgc cttcagtaaa ccataattca
     4801 cgccccgtac ttaaattggt ggcaacagcc gcaaagcgac gggaacaatt ttcgatctct
     4861 gtttccggca ttatttcgcg atattgattg aagacacgct cgccgcgcag taacccaccg
     4921 cgctgccagg agaggtccat caggcgtaaa acgtcccaat aactgaaaga ggttacccaa
     4981 tcttccagcg cagataatcg atcgcatgca taggccgcgc ccaccagcga accaattgaa
     5041 catcctgcaa cgatatcaat ttcaataccc acttttttta gcgcattaat aacgccaata
     5101 tgcgaccaac ctctcgccgc gccagatccc agcgccagcc ctatttttat ctttctcatt
     5161 attcccgcaa gattcccctg gaatgcaatc gtagccacat cgctactgct cggttaacat
     5221 agtgctactc aggcggttaa tacgccgtta ttgtttccag ggagattatt tgtgtctcag
     5281 ctttgtccct gtggtagtgc tgtcgagtat agcctatgtt gccaccctta tgtgtctggt
     5341 gaaaaggttg cacctgatcc agaacatctc atgcgttcgc gttactgcgc ttttgtgatg
     5401 caagacgctg attatttaat aaagacctgg catccctctt gtggagcagc ggcgttacgt
     5461 gccgaattga tgaccggatt tgcgcatact gaatggttgg gattgaccgt atttgaacat
     5521 tgttggcagg acgccgacaa tatcggattt gtcagttttg ttgctcgatt taccgaaggt
     5581 ggcaaaacag gtgcaataat tgaacgttcg cgatttttga aagaaaacgg tcaatggtac
     5641 tatattgacg gtacacgtcc gcagtttggt cgcaacgatc cctgcccttg tggttcaggt
     5701 aaaaaattta aaaagtgctg cggccaataa tggttgacgg tacggtttag caaacactct
     5761 caacaaggtt tttccagcaa tgcattcact ccaacgtaaa gttctgcgta ctatttgtcc
     5821 ggaccaaaaa ggtctgatcg cacgtattac caatatttgc tacaagcacg agttaaatat
     5881 cgtacagaac aatgaatttg ttgatcaccg taccgggcgc ttttttatgc gcacggaact
     5941 ggaagggatt tttaatgatt ccaccctgct ggcggatctc gatagcgcat tgccagaagg
     6001 ctccgtgcgt gagctgaatc ctgccggtcg tcgccggata gtgattctgg tcactaaaga
     6061 agcgcattgc cttggcgatt tgttgatgaa agccaattac ggcggtctgg atgtcgaaat
     6121 cgcggcagtt attggtaacc acgatacttt acgttctctg gttgagcgtt ttgatattcc
     6181 gtttgagctg gtaagccatg aagggttaag ccgcaacgag cacgatcaaa agatggcgga
     6241 cgccattgat gcttatcaac ctgactatgt ggtgctggcg aagtatatgc gggtattaac
     6301 gccggaattt gtggcacgct tcccgaataa aatcatcaat attcaccatt cattcctgcc
     6361 tgcgtttatc ggcgcacgtc cttatcacca ggcctatgaa cgtggtgtga agattattgg
     6421 cgcaaccgct cactatgtga atgacaatct ggacgaaggc ccaatcatca tgcaggacgt
     6481 tattcatgtc gatcatacct acacagctga agatatgatg cgcgcaggtc gtgacgtcga
     6541 gaaaaacgtc ttaagtcgtg cgctatacaa agtactggcg caacgcgtct ttgtttacgg
     6601 taatcggacg attattcttt aatcgccagc aaaaataact ggttaccttt aatccgttac
     6661 ggatgaaaat tacgcaacca gttcattttt ctcaacgtaa cactttacag cggcgcgtca
     6721 tttgatatga tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag cattaccccg
     6781 tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga
     6841 aggttcgaat ccttccccca ccaccatcac tttcaaaagc cccggaattc tcaaacgaat
     6901 ctgcaatcaa atattctgcc caagcgggga aggatgagaa gcttcgacca aggttcgact
     6961 tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag cgcgcagcgc
     7021 tgagtaatcc ttcccccacc accatcgctt ccttgaataa tcaaaacctg ttactccgag
     7081 ctaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc tgtaggtcgg
     7141 ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa ccccgccgaa
     7201 gcagggtcta tgtgaactaa aattcgttta gtgacgagcg cgcaccagct ggtacttacg
     7261 tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg ggaacagcag
     7321 gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac catcgaggtg
     7381 ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca gtttcatcat
     7441 ctcgctaccg tccatatgct gagcggagaa cggaatggtc agcaggccca gcgcgcactg
     7501 gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac gcacgcgtgg
     7561 gctgaacata cgacgtttca gcaaaagcac gccgccaatc agacacagca cgccgctggc
     7621 accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg cttcatacat
     7681 ccagtgcggc gtcaacatac cgaagaagtg accgacaaaa atccccagaa taccgatatg
     7741 gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg acgccgcgcg
     7801 ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga ccgccccggc
     7861 tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg ctgtcctccg
     7921 gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg atgagcagtg
     7981 attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc ttcccagaca
     8041 acatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc cgccactttg
     8101 tcgctgtcga tagcggtatt ggccaatttc agcagcagat caaacatcac ggcataacgg
     8161 ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat atctttcaaa
     8221 ccttccacgg cttcgctttg cggcagctgc gacaggtact ccagatacag cggcagatgg
     8281 tccggtaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc cagcaggtcc
     8341 accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag caacagtgac
     8401 gtggcgcggc cacggtcgaa cagttcgctg tactgggcct gggcatcgag cggatccatc
     8461 gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cttctttcga cagatttttc
     8521 gacgcagcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc atccggatat
     8581 tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt ttgctggtca
     8641 catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct gagccgtggc
     8701 agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc cgtgccagtt
     8761 cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc agataacggt
     8821 acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg gtatcaactt
     8881 taccgtcaac agtttcagca cgtttgtaat gacgcatcgc cagcatacgt ttcagcgcac
     8941 gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt accggaatac
     9001 gcaagctttc gacgtctggc agaattccgt tactgcccag ctcgcccgcg tctgctgcag
     9061 actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga tattctggat
     9121 gcagcggcag cgccagtttc cattccatcg ccattttata aaccggcgac tgctgtgcag
     9181 cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact ttcggatcgt
     9241 tcggatcgag gaacacgtcc agctgacgct ggtagagatc tttctcgttc tcggtgctgg
     9301 ctgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg atacgaccga
     9361 cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag cagaagatgc
     9421 acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg catccggtga
     9481 tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgcca tcttcttcac
     9541 gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg cagtgttcgc
     9601 acaggcgcgg caaatacatc ataaaggtgt tttcgaactg gctatacatc gccttctgga
     9661 tgttgtcgaa gttcttgtct ttcgccagtt tatcaaactc accgcccagg tcatcttccc
     9721 agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa cgcggacggg
     9781 caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgataa tcaaaatcga
     9841 acggctcgta ataatcgtcg atccccggca gatgcgggtt agcaaagatt ttacccagca
     9901 gcatggctcg gttacccatg cgcggttgca gtttgccatt gattttacgg atccagccgc
     9961 ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctggcccggc ttggtttcca
    10021 cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt ttacaggtaa
    10081 ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg acttgtgaac
    10141 gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta accagtcaat
    10201 gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg tgccgtagta
    10261 gttaaagccg tatgccagat ggacatagcc gccgatcata tgcgtcggtt tcggcgtgat
    10321 acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac caggcaggtt
    10381 aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac gctggctgac
    10441 aaccgcacgg gcagtcagtg caccgttgct gttgaagact tcaatccagt cgttatcggc
    10501 gatacccaga tctttggcat cggcttcact caaccagacc accggaccac cgcgacctaa
    10561 agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct ggtgcggcgt
    10621 caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca cttctttcac
    10681 cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat cacgcatcca
    10741 ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga tcagctcgtg
    10801 aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag accaggtcgg
    10861 gctggagata attttgcgcg gctgtgcctg aatatcgcgg aagcggatct tctcgtcttc
    10921 tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg cagcccaggc
    10981 tttcacggct acctgaccgt tggtttccgg tgccagcgtc aggatcatct ctgccgcatc
    11041 aattgcggtg ttcagcatcg gctggccttt tgccggacct tccgctttgg tgtagttgag
    11101 cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac cgccgttacc
    11161 gattttctcc atcagcgggc cgatagaggt aaagcgttcg taagttgccg gataatcgcg
    11221 ctctacgacc ataatgtgcg gtgcagtttt gcctgggatc aggtcgcatt cgcctttttt
    11281 ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcgtgct ggataggcag
    11341 cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga atttcttcgc
    11401 gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga ccgccgcaga
    11461 cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt cgtaccaggt
    11521 cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga agtccagcgt
    11581 aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt cttccggctt
    11641 cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc ccagcaggta
    11701 cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac gccagatgaa
    11761 caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac ggatggaacc
    11821 ctctttcagg gatttcaccg tatagtccac cgaattcatc ccggcttttt ccgcttcgcc
    11881 agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc ccatgcgttc
    11941 cgcacggacg ttaaagtcga tcaagtgtcc ggtatagcgg gatttgtccg ctatcggtga
    12001 cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt tatagaaata
    12061 agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg ccagcggctg
    12121 ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc cgcccccgct
    12181 ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccacgatagt tcatatcgag
    12241 gtgataccag tggttcagcc ccgcaccgac gataatcatc gaacgaccgt gcgttttatc
    12301 agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa cgccggtaat
    12361 ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg cacagttaac
    12421 gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa cggtggtcac
    12481 cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt gcagcagcac
    12541 gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt acgggaagcc
    12601 tacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg tttcttcgcc
    12661 agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc ggaagccaat
    12721 agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt tccattccgg
    12781 attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac gacctgcagc
    12841 gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt agcgacgcac
    12901 atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca ttacgtggcc
    12961 catcgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca gatcgcacag
    13021 tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt tgtaacgcac
    13081 ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac cccaggcgat
    13141 gatgtagctg gagttatacc agtcagcaga ttccggtacg tcagtttgct cgccccaggt
    13201 ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc aagtaccgcc
    13261 aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg ccggaattgg
    13321 cgagaaacca gcaacacgat ccgggccgta gtttttgatg gtgtaaacgt tagatgcggc
    13381 gatcagttcg ttcacctcct gccaagaaga acgaacaaat ccaccgcgtc cacgcgcctg
    13441 cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct caaccggatc
    13501 gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca tcatcgggta
    13561 tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg ggcagccgcg
    13621 aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct gggtttccca
    13681 ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc agtttacccc
    13741 gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat cctcccagtc
    13801 acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct tctgtttgaa
    13861 gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc ctgtcggccc
    13921 tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa cagcgtggga
    13981 attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa gatacccctt
    14041 taggataatt aacagttaca gcataacatg ctgtaaatta agatgtttta tacctgtagt
    14101 tatttaaact attaagattt ttatgcaaat gggtattaag gagtattccc catgggtaac
    14161 atattaatgg ctcttaatga aatccggcga aataagccat ataacactaa tatgttgtaa
    14221 ctaaatgacg cataaaaaaa gcgcggcatc atgccgcgca aaggataatc aaatattaag
    14281 atttattttt tagaatgccg accatatacc gcccaggtaa tcactacgca ggcgatatag
    14341 aaaatcaaaa atactttcat tgcacctact ggcgaacccg ttaatgccag cgagctacca
    14401 aacgctttcg ggataaagaa gccaccaatc gcgccaatcg cagagatgaa acccagcgcc
    14461 gctgccgtgt cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc ttctgctttc
    14521 acgcgatcca ttgtcagttt acggaagatc actgaaatca tctggaaagt ggaaccacta
    14581 cccagcccag ctgtcaggaa cagcgccagg aagaccgcga agaacgccat gaagcttccg
    14641 ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc catcagaata
    14701 aagttcacca gcgtgacacg agttccgccc agacggtcag ataatgcacc acctgcagaa
    14761 cgcgccagcg caccaataaa cggcccgaag aaagcgtatt gcagaatctg aacatccggg
    14821 aactgcgttt ttgacagcat cgcaaagccc gcggagaagc cgatgaagga gccgaaggtt
    14881 gccagataca gcaggctcat aatccacaga tgaccccgtt tgagtaccgg caactgctcc
    14941 ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa accacgccgc aatggtgaag
    15001 atggcaagga acggcaccca tatccaggac gcattcgcca gatacagctc agtcccatcc
    15061 ggctgtttga cgccctggct accaaatact gcgaaaatcg acagtgatac caccagcgga
    15121 gcaaccaact gcatgacgct gacgcccatg tttcccagac caccattcag acccagcgcg
    15181 ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg ccatactgga tgcgaagttc
    15241 gcgccagcaa agccgcatag cagagagatg atgatgaaga cgctataagg cgtggaggta
    15301 tcctgcacgg caaaacccag ccagacgcaa ggaataatca gaataccggt gctgaacgcc
    15361 gtccagcgac gaccaccgaa gataggaacc ataaaggagt atggaacacg taataacgcg
    15421 ccagaaaccg aaggcagcgc agtcaacata aatagctgat cggtcgtaaa attaaaaccg
    15481 actttcggta agttcaccgc aacagcgctg aacaacatcc atacgcaaaa cgccagcagc
    15541 agacagggaa cggaaatcca caggttgcgg ctggcaatac gttgaccgcg ttgttgccag
    15601 aacgcaggat cttccggtcg ccaatctgtt atgacagctc cagtagccct ttcggaggca
    15661 gatgagtgac tcatagacac ctctgatact cgtttcgatg tctgccacct tagtgtctgg
    15721 agctaaaggc aatttgatgt aaatcaaacg ataagataac tttatctatc attgatattt
    15781 atcattaccc atagtgagta cagtgatttc ataaaaatta tgagattttt cacggtgctg
    15841 tagaatccct acccttaccg atgtaaagcg actaaccaca cggcaaataa ggagtaactc
    15901 tttccgggta tgggtatact tcagccaata gccgagaata ctgccattcc agaatatatc
    15961 gtcacattca ttaaggttat tgctcattta aagcctgaag gaaggggttt acatgcttaa
    16021 acgttgtctc tctccgctca ccttggttaa tcaggttgcg cttattgtgt tgctttctac
    16081 tgctattgga ctggcaggga tggcggtttc tggctggctg gtgcaaggcg ttcagggcag
    16141 cgcccatgcg atcaacaaag cgggatcgct gcgcatgcaa agttaccgtc tgttggcggc
    16201 agtgccatta agcgagaaag acaagccctt aattaaagag atggaacaaa cggcatttag
    16261 cgccgagttg actcgagcag cagaacgaga cggacaactg gcgcaattac agggtttaca
    16321 agattactgg cgtaatgaac tgatccctgc gctgatgcgt gcacaaaacc gagaaacggt
    16381 gtcagcggat gtcagccagt ttgttgccgg gcttgatcag ctggtatctg gttttgaccg
    16441 caccacggaa atgcgtattg agacagtggt actggtccat cgggtaatgg cggtatttat
    16501 ggcactttta ctggtgttca ctattatctg gttgcgggcg cgactgctac aaccgtggcg
    16561 gcaactgctg gcaatggcga gtgccgtcag ccatcgcgat tttacccaac gcgcaaacat
    16621 cagcgggcgc aacgaaatgg cgatgcttgg aactgcgttg aacaatatgt ctgcagaact
    16681 ggccgaaagt tatgccgtac ttgagcagcg ggttcaggag aaaaccgccg ggctggagca
    16741 taaaaatcag atcctctctt ttttatggca ggctaaccgc cgtttgcatt cccgcgcccc
    16801 gctgtgtgaa cgcctgtcac cggtactcaa cggcttacag aatttaaccc tgctacgtga
    16861 tatcgaactg cgggtgtatg acactgatga tgaagagaat catcaggagt ttacctgcca
    16921 gccagatatg acttgtgatg ataaaggctg ccagctctgc ccgcgcggcg tattacccgt
    16981 tggtgatcgc ggcacgaccc tgaagtggcg gctggctgac tctcatacgc agtacggtat
    17041 tttgctggcg accctgccgc aggggcgtca tcttagccat gatcaacaac aactggtgga
    17101 taccctggtt gaacaactta ccgccacgct ggcgctggat cgccatcagg aacgtcagca
    17161 acagttgatc gtgatggaag agcgtgccac cattgcgcgc gaactgcatg attctattgc
    17221 ccaatctctc tcttgcatga agatgcaggt gagttgttta cagatgcagg gcgatgcgct
    17281 gccagaaagc agccgcgaac tgttaagtca gatccgtaac gaactgaatg catcctgggc
    17341 gcagttgcgt gaattgctca ccacattccg cttgcagctc accgagcctg gattacgtcc
    17401 ggcgctggaa gcgagttgcg aagagtacag cgccaaattt ggcttcccgg tgaagctgga
    17461 ttatcaattg ccgcctcgtc tggtgccttc acatcaggca atccacttgt tgcaaattgc
    17521 ccgtgaggca ttaagtaacg ccctcaaaca ttcgcaagcg agtgaggtcg tggtgacggt
    17581 ggcgcaaaac gataatcagg tcaaactgac cgtccaggat aacggctgcg gcgtgcctga
    17641 aaatgccatc cgcagcaatc actacggcat gataataatg cgcgaccgtg cgcaaagttt
    17701 acgaggcgat tgccgcgtcc gccgtcgtga atcaggtggc accgaagtgg tggtcacctt
    17761 tattcccgaa aaaactttca cagacgtcca aggagatacc catgagtaat caggaaccgg
    17821 ctactatcct gctgattgac gatcacccga tgctgcgaac tggcgtaaaa cagcttatca
    17881 gtatggcacc agatatcacc gtggttggcg aagcgagtaa tggcgaacag ggtattgaac
    17941 tggcggaatc tcttgatccc gatctgatcc tgttagatct caatatgccc ggcatgaacg
    18001 gtctggaaac gctggataaa ctgcgcgaaa agtccctctc agggcgcatt gtggtattca
    18061 gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg gatggctatc
    18121 tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt gcatcaggca gctgctggcg
    18181 aaatggtatt aagcgaagca ttaacgcctg ttctggccgc cagcttgcgc gctaaccgtg
    18241 ccactactga gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt ctcaagctga
    18301 ttgcccaggg tttgccgaac aagatgattg cccgccgcct ggatatcacc gaaagcacag
    18361 taaaagtgca cgtcaagcac atgctgaaga aaatgaagct caagtctcgc gtggaagcag
    18421 cggtatgggt gcatcaggag cgcattttct gattacggtt cccagcgcag ttcgtcgttt
    18481 gacaatgcat cgaacggttc gacaagcgtt agcgtgatct cattggagga gacacgctgc
    18541 ccctggttat cttccaccac cacagacaat cgccagtgat tgcttgcccc ttccccgttc
    18601 tgccagtcag gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc gcccggcgtc
    18661 aaacttaata tctgcgtatc gccctgccaa atcagttgcc gaataccgta acgactgcgg
    18721 atttgtaatt tcagcggcac tgtttcgcca ggttttagat cccacggcgg tgtcgccaga
    18781 aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg gtagattatt tcgctgcgga
    18841 ttgtcatagc gactaccacg taacgactga ctttcggcaa cctcgcccgc agaaagttgt
    18901 tttttgagcg gtacaccaaa gcggtaatta agattcagcc cgaggttatt ttgattttcg
    18961 ccactttccc cctgtttatg ctgggccgtc acagtgacta atggcacagg ggtgtaattt
    19021 aatcccagac tcaacgcgac gggattgtga taacccgtac cagagttaaa caaatcaaca
    19081 cgatcaccaa aatactgttc taggctgaca ctggtattga ggtgttgata gaacggcatg
    19141 cgcatccgag ctgtcaggtc gtacccgcgc gccatccgtt gttcctgcgt ggctgtctgt
    19201 tcatgccatg cagcaaacgg ctgataaaag tttgccgata atcgcaaata ttcgccccac
    19261 gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc ataaaaagtg
    19321 ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac attgctcacc
    19381 aacccattat cctgctgagt aagaccaagc tggctccagg tgagataacg atcattatct
    19441 tgtaacggca caaaccagct tccacgactg ccggtgaaat gtccttcgtt atccactttg
    19501 acgtcaacac tggcatttcc ccacggtgat agccaggact ctacgtgctg attaacctgt
    19561 tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc gccagtatcc
    19621 agcccgttat cattcatact ggtttcgccg aaatctttca caatttcagc aaagtgtttt
    19681 tccccatcat gattttcggg agccatgcct aaatccggca gaccatcatt gttattatca
    19741 aagggatttg ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc gcctgctaca
    19801 agcaagagta aataaaacgg gataatgcga ggaacgaaac ggctcaaaac gtcggtaact
    19861 tccggtaata atgaaaaacc atagccttaa agaataacga ttactggcaa cttttgtcgt
    19921 attttctcgc gctttcagga aaatccttaa aaatgtgacc gattggtcag acgctgcaga
    19981 agcagtaaga tagaagatga gtgtctaatt ccctgaatac aggagtaaat aatgcaaaaa
    20041 atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa cagcttgcgg
    20101 ctggccattg cgttacgaga gcaggagagc aatctggatc tgcgtctgtt cctgatgtct
    20161 gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa cattcagcaa
    20221 atgctggaga tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac ctgtaccgac
    20281 gggcgcggga ttagcacact tcctctgatt gatggggtgg aaatcggtac tcttgtggaa
    20341 ctggcgcaat ggacgctgtc agccgataaa gtgctcacat tttaatgttt aagggggcgt
    20401 ctgacgcccc actatgaggg aagtttactc aggcgaatcc cggccttaac acaccatccg
    20461 gataattctc cgccagtagt ttttttaccg ataccagcaa atcattcagc ccatcgtcct
    20521 gcattcccag tttgataagc tcctgttcca gtgaaaacag gtattgtgca ttggttccca
    20581 gcggaccgct cgccgccgca atcaacggcg cgatgacctg agcgcgagtg tcagattcat
    20641 attctggatg tcgcgggtcc ataataaaca caatggcgtt tactgtgcgt ccatcatcaa
    20701 gatcaagctg acaccaggtt ggcagataac agccggtaat catctcgcgc ttccacaaca
    20761 gggttagttc ctgctccagc gtctcttctg gtagtcgata ggcgacgcct gtggtgcgtc
    20821 cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc ccggcggtca
    20881 ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc ggtgcatgat tcggtaaact
    20941 ccagtgccgg attccacatc aacgatccat aaccgaagat ccacaccggt ccctcatcag
    21001 gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac cataagagtg
    21061 attcttcaat ggcaccaaat gccgttttac aatcggcatt catcaagaaa tcacgcgtta
    21121 tcaccttaca cctccgccac tgcatacttc cctgcgacaa ctttttggtg tcttccggac
    21181 atttttaccg ctcattttga aaccactaac gggacaatat gcaatccacg gaccacttgc
    21241 aaggcaacac cgcacgaata gctttaacta agctaccggt tttacgattt tttatgccat
    21301 ttatcatcat cccctttggc atattcatgc ttcacagcag cccaggccac tttatgcgcg
    21361 gtttcttcgc gactggcgtc atcacgccga tcttctttat ctttatattg atcccatgcg
    21421 ctgttgaacg cttctttata gatatcctgg gcatgagacg gtagaacgtg ctttacgctt
    21481 tccggcagat cgcttttcgt tttatacggc atcgttgact ccttttacaa agttgaacat
    21541 taagtgtggt caacaatatc tactcttgcc aaaacataag aattttcatg tcagactgtt
    21601 cttaagaaac gctaaaacaa acattgtcta aacgtaatac ttatttttat gccccaatga
    21661 gtaaaaaatc gacagcgggg taaaaattcg taaaatgtta ccgatatcca gacactttct
    21721 gttatttttc ctacctattt tttacatttt cgataaattc atctgcaaag ggataaccat
    21781 aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag gagacttcgc ttattttccc
    21841 ggttctggcg ctggtagtgc tgttcctgtg gggaagtagc cagacactac cagtggtcat
    21901 tgccatcaat cttcttgcgc ttattggtat tttaagtagc gcctttagtg ttgtccgtca
    21961 tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc ttagcctttc
    22021 agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg acgccgcgcc
    22081 aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg ggctggttgg
    22141 cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc tgtttggtat
    22201 caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat ttccaatggc
    22261 tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa tttctgcggc
    22321 aatgtatagc gtatttttgc tgatccagac caaaacgcat caaagtttgt ttgtctacga
    22381 gcacgaagat gacagtgatg acgacgaccc gcaccacggt aaaccgtctg cccatagcag
    22441 cctgtggcat gctatctggt tgattatcca tctgattgcc gttattgcag tcaccaaaat
    22501 gaacgccagc tcgctggaga cattgctcga cagcatgaat gcccctgttg cctttactgg
    22561 cttcctggtg gcactgttga ttctgtcgcc ggaaggttta ggtgcgttaa aagcagtgtt
    22621 gaacaaccag gttcagcgcg cgatgaatct gttctttggt tcagtgttag caacaatttc
    22681 gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat tgcagtttgc
    22741 acttggtgcg ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc atatctcctt
    22801 ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt ttgccgccta
    22861 tttgatgacg atatttgcct gacagttata tctcctccga tgacggaaat cgggagagat
    22921 aagataaatt cctgcttatt gtatttcttc atactacctg gacagctcgt taatcagaat
    22981 tgcgagcctg taccacaacc tgcacgaaac cactgcaaca gctgttgaaa agccggaatg
    23041 tactctttat tgcaaacatt tcagggatga agtgaagatg aattgag
//
LOCUS       DADLSB010000051        22903 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.051, whole genome shotgun sequence.
ACCESSION   DADLSB010000051 DADLSB010000000
VERSION     DADLSB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.051"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            106..327
                     /locus_tag="JN331_001962"
     CDS             106..327
                     /locus_tag="JN331_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260115.1"
                     /translation="MTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQEGMHEQAIKIA
                     LRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(368..838)
                     /locus_tag="JN331_001963"
     CDS             complement(368..838)
                     /locus_tag="JN331_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7260116.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(893..2095)
                     /locus_tag="JN331_001964"
     CDS             complement(893..2095)
                     /locus_tag="JN331_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY7260117.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPPEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGNEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(2182..2724)
                     /locus_tag="JN331_001965"
     CDS             complement(2182..2724)
                     /locus_tag="JN331_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260118.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            3003..3428
                     /gene="nudI"
                     /locus_tag="JN331_001966"
     CDS             3003..3428
                     /gene="nudI"
                     /locus_tag="JN331_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY7260119.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(3467..4069)
                     /locus_tag="JN331_001967"
     CDS             complement(3467..4069)
                     /locus_tag="JN331_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY7260120.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSEGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            4377..5516
                     /gene="arnB"
                     /locus_tag="JN331_001968"
     CDS             4377..5516
                     /gene="arnB"
                     /locus_tag="JN331_001968"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY7260121.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            5520..6488
                     /gene="arnC"
                     /locus_tag="JN331_001969"
     CDS             5520..6488
                     /gene="arnC"
                     /locus_tag="JN331_001969"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY7260122.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSTHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            6488..8470
                     /gene="arnA"
                     /locus_tag="JN331_001970"
     CDS             6488..8470
                     /gene="arnA"
                     /locus_tag="JN331_001970"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY7260123.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHTARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            8467..9357
                     /locus_tag="JN331_001971"
     CDS             8467..9357
                     /locus_tag="JN331_001971"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY7260124.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            9357..11009
                     /gene="arnT"
                     /locus_tag="JN331_001972"
     CDS             9357..11009
                     /gene="arnT"
                     /locus_tag="JN331_001972"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY7260125.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            11006..11341
                     /gene="arnE"
                     /locus_tag="JN331_001973"
     CDS             11006..11341
                     /gene="arnE"
                     /locus_tag="JN331_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY7260126.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            11341..11727
                     /gene="arnF"
                     /locus_tag="JN331_001974"
     CDS             11341..11727
                     /gene="arnF"
                     /locus_tag="JN331_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY7260127.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(11721..11993)
                     /gene="pmrD"
                     /locus_tag="JN331_001975"
     CDS             complement(11721..11993)
                     /gene="pmrD"
                     /locus_tag="JN331_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY7260128.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(12098..13453)
                     /gene="menE"
                     /locus_tag="JN331_001976"
     CDS             complement(12098..13453)
                     /gene="menE"
                     /locus_tag="JN331_001976"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY7260129.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(13450..14412)
                     /gene="menC"
                     /locus_tag="JN331_001977"
     CDS             complement(13450..14412)
                     /gene="menC"
                     /locus_tag="JN331_001977"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY7260130.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(14412..15269)
                     /gene="menB"
                     /locus_tag="JN331_001978"
     CDS             complement(14412..15269)
                     /gene="menB"
                     /locus_tag="JN331_001978"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY7260131.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(15284..16042)
                     /gene="menH"
                     /locus_tag="JN331_001979"
     CDS             complement(15284..16042)
                     /gene="menH"
                     /locus_tag="JN331_001979"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY7260132.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(16039..17709)
                     /gene="menD"
                     /locus_tag="JN331_001980"
     CDS             complement(16039..17709)
                     /gene="menD"
                     /locus_tag="JN331_001980"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY7260133.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFGSHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     VGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSCLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(17798..19093)
                     /gene="menF"
                     /locus_tag="JN331_001981"
     CDS             complement(17798..19093)
                     /gene="menF"
                     /locus_tag="JN331_001981"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY7260134.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAALGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAVLRTLLQME"
     gene            complement(19172..19477)
                     /gene="elaB"
                     /locus_tag="JN331_001982"
     CDS             complement(19172..19477)
                     /gene="elaB"
                     /locus_tag="JN331_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY7260135.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(19532..19993)
                     /locus_tag="JN331_001983"
     CDS             complement(19532..19993)
                     /locus_tag="JN331_001983"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7260136.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            20058..20975
                     /gene="rbn"
                     /locus_tag="JN331_001984"
     CDS             20058..20975
                     /gene="rbn"
                     /locus_tag="JN331_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY7260137.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            21163..22382
                     /gene="elaD"
                     /locus_tag="JN331_001985"
                     /pseudo
     CDS             21163..22382
                     /gene="elaD"
                     /locus_tag="JN331_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         5672 a         6027 c         5589 g         5615 t
ORIGIN      
        1 ttgcgcatgc tggagcaggg ctttgaacgt gagattgtgc tggcggcaac ccaactcact
       61 gatgctgata ttccgaactg ttattaaaag gagagggaga gattgatgac tattgctgaa
      121 cggctacggc aggaaggaca tcaaattggc tggcaagaag gtaaattagt aggtttgcag
      181 caaggtaaat tagaaggttt gcaggaaggt atgcatgaac aagccattaa aattgctttg
      241 cgcatgctgg aacagggcat tgatcgtgac caggtgctcg cggccaccca gctaagcgaa
      301 gccgatctgg cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc
      361 tttgatatca ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca
      421 gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg
      481 ctccccacgc caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt
      541 tacccgcagc acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc
      601 cggcgttatc cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat
      661 caatcccttc gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct
      721 gcactaacag acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc
      781 gcgcagcccg tgccacactg gccccaacac cacgctcacc gtagggaggg ttggacaaat
      841 tttgccggag ggggaatgtg ccggtgcagt aaaccggcac agagtggcga aatcagacgg
      901 ataaagtcat ggactcaaca acctcaatcc agccatgctc actagcgatg tcctggccgt
      961 ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca cacttcctga cggatagcca
     1021 ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa agtgccgtct ggcgtggcta
     1081 gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc cagtgccagc ccggcaaaat
     1141 ggttggcccg ccgttctgta atccagtgcg cagtttgcgc cagggtttct tcctgtgaag
     1201 gaaccacttc acacgccagc aatggagcac ctgcgcgaga aagttgcaaa gccaataaac
     1261 caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg gttttgcaat tcgcgactga
     1321 tctgcgcggg cagtccttcg ttgccttcga aaatcacgct ctgtccggca acacgtttaa
     1381 cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc cggtccggtg agtttcagtt
     1441 cgatgatcgg cattgaggag cgatagccca ttgttacgcc cggcggcagt tgtagagtgt
     1501 ccaggctttg tgccagatcg ctttccgaac gaccaaaggt agttaaacgc agacaaaccg
     1561 gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag gatttcgtgc tcgaccatca
     1621 ccttaaattc tggcggtacg ccgggagtaa agaacatcag gcaacgatta agctgcacgg
     1681 caaaaccaca ggcggtgcct accgggttat tgataaattc agcactggca ggcagctccg
     1741 cttgtttacg gttgctcggt gccattactc gtccacgttc gtggaaatag cgttccatct
     1801 ctttgagcca ggcttcatgc agcaccaggc cttcaccttt tgctgtcgca gcggcgagtg
     1861 cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac gatcagcaca tcggcgtgct
     1921 gactacgttc gcgaagaatg gtgactaagt catcaaggtt atcccccacc gtattgcggc
     1981 gagataatgg cagcccctga tgaaagaaaa aatcggccag ccaggcagcg ttagtgtcaa
     2041 cgatttgccc gtataacact tcatccccgg tggatagcat ttccactttt aacatggtta
     2101 tctcctgctt ttgttatcga atcactataa cgcaaaggga gataaggcgt agtaagaaac
     2161 tgaatgccgc gccggaaggg atcagaaact ggcgttaacc ccaacatacg ggccgtcagc
     2221 cacggcgttg tcgcggttac cgtctttacc cgacagattc aggtagcgat aacccgcctc
     2281 aatactgact ggacgcataa tggtgtaacg cgcgccagca ttcgcttcct cataactttg
     2341 aataccgctg gagagcgaat ccggagagta gtaatactcg ccaaacaaac ggaagctgtt
     2401 gccaattttc cactgcaaac cacctcctac cgctgcggca taaccttcat cgccgtaatt
     2461 cgggttggtg tacacgcctt ttccgccaac ggtcgccatt aacggcccga gaggcagatt
     2521 caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg ttatgtgtcc agttaccgct
     2581 taaagccagg cccgtcgatt cagtcccaaa accgacacca atattggtgt attctttacc
     2641 cgcctggccg ctgatgctca ttgcattgac cgatgcagaa accaacagca ttccggccag
     2701 acctgctagc gcaatttttt tcattatccc ctttcccaca agcaattaat caaaattccc
     2761 aaagctgcgg gattgtactg cctggcacag aggattcaat gagcgaaaaa agggatctag
     2821 tcacttattg taactaaata cttcttaaat aattaaacgc cgaaaaaata aacacacttt
     2881 accgatacac acaaaaataa taataagtgc agttatcatt gcaaaagaga ttactgtcac
     2941 tttcacatat ttctgagtta ccgtattctc atggtttctt tttatttata aggaataccc
     3001 ctgtgcgaca acggactatt gtatgccctt tgattcaaaa tgatggtgct tatttgctgt
     3061 gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg ggcgctttcg ggtggcggcg
     3121 tggagtctgg cgaacgaatt gaagaggcac tacgccgcga aattcgcgaa gaactgggag
     3181 aacagctgct tttgacagaa atcacgccgt ggaccttcag cgatgatatt cgcaccaaga
     3241 catatgcaga tggtcgcaag gaagagattt atatgattta cctgattttt gactgcgttt
     3301 ctgccaaccg agaagtgaaa ataaacgaag agtttcagga ctacgcgtgg gtaaaacctg
     3361 aagatctggt gcattatgat ttaaatgttg ccacccgaaa aacgttacgt ttgaaaggtc
     3421 ttctttaaca atgatgacag ccatcactgg ctgtcagatt gaaaatttag tggttaacga
     3481 attccccatc cagataaact ttgccttttt ccacatgcat gactaaacca tccagataat
     3541 caggtttaaa tgtcgcatca cgcttatttt tagcaatata tgtcaggcaa tgattatggg
     3601 tgaaaataac gatattttta tcaggcgctt tgctttgtaa gtccttaatt gcactataaa
     3661 tctcattacc gcactgaaga agtcgtttat ctaccgtcag ctttttaccc tcggaaaacc
     3721 aggtcgccga ctgaatggtc cggacggtat tactggaata aagatcgaaa tcagggatgt
     3781 cagcgctaaa agcgttgccc agttcacggg catcctgggt acctttaacc gtaatacctg
     3841 ttttatctga caagcattga ttggttgaac gatcgcaacg ttcagcatgg cgaaacaaaa
     3901 cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt attgtcgata cgtggcaaac
     3961 cattgctact ccaggcggca tgagtaccca gtccggcaat tgcagcgagc gccagtaaaa
     4021 tgatgaaata tttttttgac ttcaatgatg agcggcaaaa agctaacacg taaactccac
     4081 ctatagacaa gcgcaaccag acaattaccg tgaaattgag cgacatttct ggcgataatt
     4141 agcagttggt gtaatattaa aaatcctacg atgtcggcaa aatgcctcaa atttttgcca
     4201 aatgcaaagc ctaaataaga aaaaatataa aaatttcaat atttacgtct aatattagtt
     4261 tcttaaggtt aagttaatat tctatcctta aaatttcgct ccaaatggca aaatatacac
     4321 aacactcttt atagcaaata taagtggaca ggtattcaat ggcggaagga aaagcaatgt
     4381 cagaattttt gcctttttcg cgaccagcaa tgggcgtgga ggaactcgct gcagttaaag
     4441 aggttctcga atccggttgg atcacaaccg gtccgaagaa tcaggcgctt gagcaagcct
     4501 tttgccagtt gacgggaaat cagcatgcca tcgcggtcag ttcagccacc gccggaatgc
     4561 atatcacgct aatggcgttg gaaattggca agggcgatga agtgattacg ccttccctga
     4621 cctgggtttc aaccctcaat atgatttcct tgctgggtgc aacgccggta atggtggatg
     4681 tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga atcagccatt acgccacgca
     4741 ctaaagccat cattccggtg cattatgccg gtgcgccagc agatattgac gccattcgcg
     4801 ccattggcga acgttacggc atcgcagtta tcgaagatgc tgcccatgcc gtcggtacgt
     4861 attacaaagg gcgacatatt ggcgcaaaag gtaccgctat tttttcattt catgccatta
     4921 aaaatattac ctgtgctgaa ggtggcctga ttgtaactga taatgaaaac cttgcccgcc
     4981 agctacggat gctgaaattt cacggtctgg gtgtcgatgc ctatgacaga caaacctggg
     5041 gccgtgcacc gcaggctgaa gtcttaacac cgggctataa gtacaatctg accgatatta
     5101 acgccgcgat tgccctgaca cagttagtca aattagagca cctcaacacc cgtcggcgcg
     5161 aaattgccca gcaatatcag caagcactgg cagctctccc ctttcagcca ttaagccttc
     5221 ccgcctggcc gcacgttcac gcctggcatc tgtttattat tcgtgtcgat gaacaacgtt
     5281 gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga aagaggcatt ggtaccgggt
     5341 tacatttccg cgccgctcac acacaaaaat attatcgcga gcgttttccc acgctgtcgt
     5401 taccgaatac cgaatggaat agcgaacgca tctgttcgtt gccgctgttc ccggatatga
     5461 ctaccgccga tgccgaccgc gtcatcacag cccttcagca actcgcagga caataagcca
     5521 tgtttgaaat ccaccctgtt aagaaagtct cggtggttat tcccgtttat aacgagcagg
     5581 aaagcttacc ggaattaatc aggcgcacca ccacagcctg tgaatcgttg gggaaagagt
     5641 atgagatcct gctgattgat gacggcagta gcgataattc cacgcatatg ctggtcgaag
     5701 cctcacaagc ggagaacagc catattgtgt ctattttgct taaccgcaat tacgggcaac
     5761 attcagcgat tatggcggga ttcagtcacg ttactggcga cttaattatt acccttgatg
     5821 ccgatctcca gaatccgcca gaagaaatcc cccgcctggt ggcaaaagcc gatgaaggtt
     5881 acgacgtggt agggactgta cgccagaacc gccaggacag ctggtttcgt aaaacagctt
     5941 cgaagatgat taaccggctt attcagcgca ccactggcaa agcgatgggt gactacggtt
     6001 gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat gttgcactgc catgaacgca
     6061 gcacctttat cccgattctg gcgaatatct tcgcccgccg tgcaattgaa attccagtac
     6121 atcatgccga gcgtgagttt ggtgaatcca aatacagttt tatgcgcctg attaatttga
     6181 tgtacgacct ggtgacctgc cttaccacaa cgccgctacg tatgctgagt ctgctcggca
     6241 gcattattgc gattggaggt tttagcattg cggtgctgct ggtgatttta cgcctgacct
     6301 tcggaccaca atgggcggca gaaggcgtct ttatgctatt tgccgtgctg tttactttta
     6361 ttggcgctca gtttatcggc atgggattac tcggtgaata tatcggcagg atctacaccg
     6421 atgtccgcgc ccgcccccgc tattttgttc agcaagttat ccgtccatcc agcaaggaaa
     6481 atgaataatg aaaaccgtcg tttttgccta ccacgatatg ggatgcctcg gtattgaagc
     6541 tctgctggct gccggttacg aaattagcgc catttttacc cataccgata atcccggtga
     6601 aaaagccttt tatggttcgg tggctcatct ggcggcggaa agaggcattc cggtttatgc
     6661 gccggataac gttaatcatc cgctgtgggt ggaacgcatt gcccaactgt caccagaggt
     6721 gattttctct ttttattatc gccatcttat ttgcgacgag attttgcagc tcgctcccgc
     6781 aggtgcattt aatctgcacg gttcgctgtt accaaaatat cgtggtcgcg cgccgctgaa
     6841 ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca ttgcaccgaa tggtaaaacg
     6901 tgccgatgct ggggccattg tggcgcaact gcgcgttgcc attgcgccag acgatatcgc
     6961 tattacgctg catcataaat tgtgccatac cgcgcgccag ctactggaac agacattacc
     7021 cgccattaaa cacggtaata ttctggaaat cgcccagcgc gaaaacgaag ccacctgttt
     7081 tggtcgcaga acgccggatg acagtttcct tgaatggcat aaaccggcat ccgtactgca
     7141 caacatggta cgtgccgttg ccgatccgtg gccgggtgcc ttcagctatg ttggcaatca
     7201 gaaattcacc gtctggtcgt cgcgtgttca tcctcatgcc agcaaagcac agccggggag
     7261 cgtgatttct gttgcgccac tgctgattgc ctgtggcgat ggcgcgctgg aaatcgtcac
     7321 cggacaggcg ggcgacggca ttactatgca gggctcgcaa ttagcgcaga tgctgggcct
     7381 ggtgcaaggt tcacgcttga atagccagcc tgcctgcacc gcccgacgcc gtacccgggt
     7441 actcatcctc ggggtgaatg gctttattgg caaccatctg acagaacgcc tgctgcgcga
     7501 agatcattat gaagtttacg gtctggatat tggcagcgat gcgataagcc gttttctgaa
     7561 tcatccacat tttcactttg tcgaaggcga tatcagtatt cattccgaat ggattgagta
     7621 tcacgtcaaa aaatgtgatg tcgtcttgcc gctggtggcg atagccacgc cgattgaata
     7681 tacccgcaac ccgctgcgcg tatttgaact cgattttgaa gagaatctgc gcattatccg
     7741 ctactgcgtt aagtaccgta agcgaatcat cttcccgtca acttcagaag tttatgggat
     7801 gtgtagcgat aaatacttcg atgaggacca ttctaattta atcgtcggcc cggtgaataa
     7861 accacgctgg atttattcgg tgtcaaaaca attacttgat cgggtgatct gggcctatgg
     7921 cgaaaaagag ggtttacagt tcaccctctt ccgcccgttt aactggatgg gaccacgact
     7981 ggataacctt aatgcagcgc gaattggcag ctcccgcgct attacgcaac tcattctcaa
     8041 tctggtagaa ggttcaccaa ttaagctaat tgatggcgga aaacaaaaac gctgctttac
     8101 tgatatccgg gacggtatcg aggcgttgta ccgcattatc gaaaatgcgg gaaatcgctg
     8161 cgacggcgaa attatcaaca ttggcaatcc tgagaacgaa gcgagcattg aggaactggg
     8221 cgagatgctg ctggcgagct tcgaaaaaca tccgctgcgc catcatttcc caccgtttgc
     8281 gggctttcgc gttgtcgaaa gtagcagcta ctacggcaaa ggatatcagg acgtagagca
     8341 tcgtaaaccg agcatccgca atgcccaccg ctgcctggac tgggagccga aaattgatat
     8401 gcaggaaacc atcgacgaaa cgctggattt cttcctgcgc accgttgatc ttacggataa
     8461 accatcatga ccaaagtagg cttacgcatt gatgtcgata cctttcgtgg cacccgtgaa
     8521 ggcgtgccgc gtctgctgga aatcttgagt aagcataata ttcaggccag catttttttc
     8581 agcgtcggcc cggacaatat gggccgccat ctctggcgac tggtgaagcc acagtttttg
     8641 tggaagatgc tgcgctcaaa cgcggcatcg ctttatggct gggatatttt actggcaggt
     8701 acggcctggc caggtaaaga gattggtcat gccaatgccg atatcattcg tgaagcggct
     8761 aaacatcacg aagtcggcct gcacgcctgg gatcaccatg cctggcaagc ccgtagcggt
     8821 aactgggatc ggcaaacaat gatcgacgat attgcacgcg gtcttcgcac tctggaagag
     8881 attatcggtc aaccggtaac ctgttctgcc gctgcgggct ggcgtgccga ccagaaggtg
     8941 atcgaagcaa aagaagcgtt ccatttgcgc tacaacagcg attgtcgtgg ggccatgccg
     9001 ttccgtccac tgctcgaatc aggaaaccct ggcactgcgc aaattccggt gaccttaccc
     9061 acctgggatg aagtgattgg tcgggatgtg aaagcagaag attttaacgg ttggttactc
     9121 aaccgcatcc tgcgagataa aggcacgccg gtttatacca ttcatgcaga agttgaaggc
     9181 tgcgcttatc agcataattt tgtggatctc ctcaaacgcg cagctcagga aggcgtgaca
     9241 ttttgccctt taagcgaact gttatcagag acgttgccgc tcggacaagt tgttcgcgga
     9301 aatattgccg gacgtgaagg ctggctgggt tgccaacaaa ttgcgggtag tcgctgatga
     9361 aatcggtacg ttaccttatc ggcatcttcg cgtttattgc ctgctattac ctgttaccga
     9421 tcagcacgcg tctgctctgg caacccgatg aaacgcgtta tgcggaaatc agtcgagaaa
     9481 tgctggcatc cggcgactgg attgtgcccc atctgttagg gctacgttat ttcgaaaaac
     9541 ccattgccgg atactggatt aacagcattg ggcaatggct atttggcgcg aataactttg
     9601 gtgtgcgggc aggcgttatc tttgcgaccc tgttaactgc cgcgctggtg acctggttta
     9661 ctctgcgctt atggcgcgat aaacgtctgg ctctactcgc cacagtaatt tatctctcat
     9721 tgtttattgt ctatgccatc ggcacttatg ccgtgctcga tccgtttatt gcattctggc
     9781 tggtggcggg aatgtgcagc ttctggctgg caatgcaggc acagacgtgg aaaggcaaaa
     9841 gcgcaggatt tttactgctg ggaatcacct gcggcatggg ggtgatgacc aaaggttttc
     9901 tcgcccttgc cgtgccggta ttaagcgtgc tgccatgggt agcgacgcaa aaacgctgga
     9961 aagatctctt tatttacggc tggctggcgg ttatcagttg cgtactgacg gttctcccct
    10021 ggggactggc gatagcgcag cgggagcctg acttctggca ctattttttc tgggttgagc
    10081 atattcaacg ctttgcacta gatgatgccc aacatagagc tccgttctgg tactacgtgc
    10141 cggtcatcat tgccggtagc ctgccgtggc tgggattact ccccggtgca ctgtacacag
    10201 gctggaaaaa ccgcaagcat tccgcaaccg tctatttgtt gagctggacg ataatgccgc
    10261 tgctgttttt ctccgtcgct aaaggcaaat tgcccaccta tattctttct tgctttgcac
    10321 ctctggcaat gctgttggcg cattacgctt tgctggcagc aaaaaataat cctctggcgc
    10381 tgcggattaa tggctggatt aacatcgctt ttggcgtcac tggcattatt gccacatttg
    10441 tgatctcccc ctggggacca atgaacacgc cggtgtggca aaccttcgag agctataaag
    10501 tcttttgtgc ctggtcgatt ttttcgctat gggcattttt cggctggtac accttaacaa
    10561 acgtcgaaaa gacctggtct ttcgccgcgc tttgcccgct ggggctggcg ttgctggtag
    10621 gattttcaat tcctgacaga gtcatggaag gaaaacatcc gcaatttttt gtcgagatga
    10681 cacaagaatc acttcagcca agccgctata ttcttactga tagcgtcggt gttgccgcag
    10741 gtctggcatg gagcctgcaa cgcgatgaca tcatcatgta tcgccagaca ggtgagttga
    10801 aatacggcct taattatccg gatgcgaaag ggagatttgt cagcggtgat gagttcgcaa
    10861 actggcttaa tcaacatcgt caggagggga ttattacact cgtgctttcg gttgaccgcg
    10921 atgaagatat caacagtctc gccattccgc ccgcagatgc catcgatcgt caggagcgtc
    10981 tggtgctgat tcagtatcgt cccaaatgat ctggctaaca ttagtctttg ccagcttgct
    11041 tagcgttgcc gggcagttgt gtcagaaaca ggcaacctgc tttgtggcga taaacaaacg
    11101 gcgcaaacat atcgtgctgt ggctgggact ggcgctggct tgtcttggtc ttgccatggt
    11161 gctctggctg ctggtcttgc agaacgtacc ggtaggcatt gcctacccga tgttaagtct
    11221 gaattttgtc tgggtgacgc tggctgcagt aaaactgtgg cacgaaccgg tatcgccgcg
    11281 tcactggtgt ggggtggcgt tcattattgg tggcattgtg atcctcggga gtacggtgta
    11341 atgggcctga tatggggatt attcagcgtc attattgcct cagttgcgca attaagcctg
    11401 ggttttgcgg caagtcatct gccgccgatg acgcacttat gggattttat cgcggctctg
    11461 ttagcttttg gcctggatgc ccggatactg ttactcgggc tgctgggtta tctgctttcc
    11521 gttttttgtt ggtataaaac gctacataaa cttgcgctaa gcaaagccta tgccttgctc
    11581 agtatgagtt atgtgctggt atggattgct tcaatggtac ttcccggctg ggaagggact
    11641 ttttcgctga aagcactact gggagtagcc tgtattatga gcgggttgat gctgattttc
    11701 ctgcccacga caaaacaacg ttactgagtt ttccctgccg ctgtacactg ccgttcccac
    11761 tcatcggggc tataactgct tgccgagagg actttcaccg tatgcagctt ttcacgatta
    11821 atacaataaa gcgcattctg caaaggcgag agtaaatccc ccactttcac ggcgaagtca
    11881 ctcttcactt cggcgatcat ttttatcgcg ccgccagcat cgcagagcat aagtacatgt
    11941 ctgttatctt gtttattgca gcacgatttt ttgtgccagc cattccattg cattatcctg
    12001 tttgctaaga gttttcacat caatttacag attaacagat aaactcttta tcaacggcaa
    12061 tggaatggca gaaatgagaa gtgaaacgga gggttaatta ttgctgacgt tgcacccact
    12121 cttttagcgc ctgacgtgaa attttaatac cgccgttttt cagctccggc ggtaaagtca
    12181 gccagcgcac cggttgttga aaacgagcca gcttatcttt cacccattcg ccaagatcaa
    12241 cggtttgctg gtcatactcc atcaccgcta ccggccgatg accaaactcc ttgtcggcaa
    12301 cgggaacgat aaacacctgc aaaaccgcag gatgtgcagc aataacacgc tcgacttctt
    12361 ccggctgaat accctcaccg ccactgaaga acagattgtc taaacgtccg acaatggtca
    12421 gcttgccatt atgcatctca ccgcgatcgc gcgtagcgta ccagccttcg tcattaacca
    12481 gtgaaaccag ttgcccgtta cgccagtaac cttctgccat actggcagcc cgcagccaca
    12541 cttcattatt aacgattttc acttcccgac ccggcagcgg caaaccaacg tctgccaggc
    12601 cgtcggcttc tttcgcacat accgtggagg caaactcggt cagaccatag ccgcaaaagc
    12661 aacgaatccc ctgctcgcgc gcctgttccg tcaactcgac cgggatagcc gcgccgccaa
    12721 gtaacaccgc tttcagtgaa acggaactgc ggttaaccag caaacgccag agttgtgttg
    12781 gcaccagtga agcgtgagta cagcctgcca gcatttgctc caatggctgt ttatcacgca
    12841 ccgtcatccg cgcaccagcg tataaccagc gccacataat tccctgaccg gagacgtgaa
    12901 acagcggtaa agagagcaac cagtcatcgt gatcgccaaa cggaatcagc gataacacac
    12961 cttgcgcact ggcaagatgg gcctgataag tatggacagc ggctttcggc aaaccggtag
    13021 aacccgaggt caacgtcatt gagcacagac gcgtcggctg ccacgcagcg gcatgtacgc
    13081 cttcaaccag ctgaatgtgc agcgacgtta atgccggaaa cgtgttttcc ccatccggca
    13141 ccagcgcaaa ttgcagcgtc agattgggca gcaattcttc aagcaacggt tgcggcagct
    13201 gagggttcac gggcaacacc cgcgccccgc attgcagtaa cgccagccag gcgagcagcg
    13261 tttgcggcgt attccacgcc cgcaacatca cgccgctgcc ctcaaccaca ccctgcgccg
    13321 caaagcccgt agctaactca tcgacgcgag cacaaagctc gcgccagttg agttgctcgt
    13381 cattaagacg taaggcgatg gcttctcccc gcacttgccg ccagtgacgc cacggccagt
    13441 cagagaagat cataacaact gctccagtac atcagcgtcc actaacggca gtgggctccc
    13501 cggccagcga cgaacctgct gcgcctgcat cagatccagc gtgtccagtc ccggaatggt
    13561 gtctggcgtt aaccaggcag caatccgcgc cagttgcgtt agacctaagc tcgactcaat
    13621 ggaagaactg atcaccgccg tcagccccag cgcatgcgcc gcctgtacct gctcgcgtac
    13681 tttttccaga ctgcccgtga gcgtgggttt gataactacc gcgcgcacgc cctcttcagc
    13741 cacaaaggca aaatccggct cgcgcaggct ttcatcccag gcaatggcaa tgccggtttc
    13801 acgggcaaac gctcgcgaat catcgcgggt tttgcacggc tcttcgagaa acgcgatgcg
    13861 gtggcgataa tcagggttaa catatttggc aaactgctga cctttcagcg gtgtccaggc
    13921 gcgatttgcg tcaagacgca aatgcagatc cggaattgcc tccagcaaca gattcaccac
    13981 catgccgtcg cgcaccgctt cgtacaatcc cactttgacc ttcgccactt tctcgcccgg
    14041 catatcggca agtttgagga tcagatcgtc cggatcgcca ttacacagcg gtgccgcacg
    14101 gtagttggct gcttgcggca acgtatctgt cagttctgcc aatgcacagc ttacgccaaa
    14161 ggccacggaa ggcatctgcg gtagctcgca atcgcctgcc agccagttat ttacccaggc
    14221 aagcagcacg ctttgcgcct cttcccaggt ttcctggctg aagcccggca gtggggagat
    14281 ctccccccac ccttcgcgct cgccctcacg caggcaaacg tacagcccgt cgcgggtttt
    14341 taaccgcctg tcgcgcagaa ccacccccgc gtccatgggg atctgccagc ggtatacctg
    14401 cgcgctacgc attacggatt ccgtttaaat ttgctgaaat caggctgacg tttctggttg
    14461 aaagcgttgc gaccttcctg accttcttcc gtcatgtaga acagcatggt ggcgttgccc
    14521 gccagctcct gcagccccgc ctgcccgtca cagtcggcgt tcagcgcagc tttcaggcag
    14581 cgcagcgcca tcgggctgtt ttgcagcatt tcgcggcacc aacggacggt ttctttttcc
    14641 agatccgcca gcggtaccac ggtgttgaca aggcccatat ccagcgcctg ttttgcgtcg
    14701 tactgacggc acaggaacca gatttctcgc gcttttttct gcccgacgat acgagccatg
    14761 taggaagcgc cccagccgcc gtcgaaggaa ccgactttcg ggccagtctg accgaagatg
    14821 gcattatctg ctgcgatagt caggtcgcac atcatgtgca gaacgtgacc accgccgatg
    14881 gagtagccag ccaccatcgc gacaaccggt ttcggacagg tacggatctg acgctggaag
    14941 tccagcacgt tcaggtgatg tacgccggaa tcatctttat agccgccgta atcaccacgc
    15001 actttctggt caccaccgga gcagaacgct ttatcgcctg cgccagtcag aatgatcacg
    15061 ccgatattgt cgtcataacg cgcatctgcc agtgcctgga tcatctcttt taccgtcaga
    15121 ggacggaagg cattgcgcac ctgcggacga ttaatggtga tttttgcgat accgtcggtg
    15181 gatttttcat aacgaatgtc ctcgaaacct tcggagcagt cgtgccattc aaccggtgcg
    15241 taaggcattg cttcatcagg ataaatcata ttgtgtcctt tagtcagaaa cgcaagatct
    15301 gcgccagact tgcgattacg ccagcgggat tttcccgatg cgcgttatgt ccggcgcgag
    15361 gaatgacatg gcagtcggca gccagttccg ccgccagggc gcggaatttg ctgtcacgtt
    15421 caccacataa ataataaaac gcaaatgtgc gggcgctaag gttagcacgt aaatcaggct
    15481 ggacggcgag agaagtcgcc tccagcatgg cggcaagggt tgcgccatta ttgttgctgc
    15541 gcagcgccac cagctcccgg cgttgatcgt cattgagcga ggcaaaaaca ggctgttgat
    15601 accagtcggc aaataccgcc gttaacggtt ctgtgcgaaa acgctgcgcc cattggcgat
    15661 cggaacgctg acgttccgca cgttgttcag cattttgcag ccccggatgc ccgccttcga
    15721 caataacccc acaaagcccc gccagcccct ggcaagccgc catcatcgcc acccgtccgc
    15781 caagcgagta ccccaccagc cagaagtcaa ggatattgta actaaccaag gttttacgca
    15841 gtaagtcggt gacatcatca aatccatcga cgctaatcgc cgccgaacca ccatgacctg
    15901 ggagatcaac atacaaccgt gagtagtcgg caaacgcctc gcccacttct tgccattcgt
    15961 ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg taaacctggt tttccgtgtt
    16021 ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc agaagttgct ggagcgtttg
    16081 cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg gtggttggcg tgcgccaggc
    16141 gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga cgatgatatt tcagctcgaa
    16201 catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc agatagaaac gctcacgctc
    16261 gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg ttgttgttca ccacaattaa
    16321 taccagcggc gcagaaacct gacgcaataa cgccagcgcg ttgagatcgt aaagtgcgga
    16381 gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc cgctgaacgc ctgcggcggt
    16441 cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg tacaccgggt aacctgccgg
    16501 aagttgcgaa agcgcatcaa tcagacgtac caccaggctg ttaccaacaa acaattgccc
    16561 ctgttcaggc agatagtcgc tgatacgatg cgccagttgc gcttcgccaa acgcatcacg
    16621 gcgggcaata accgcctgca ttgcctgttc cgccaggcgc gggatttcaa cgcaccaggg
    16681 ctggcgtttt tctgccggat gcagctccag ccagtcggca atattggcaa ttaagcgacg
    16741 tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca tcaacaatcc agtactcttc
    16801 tggttcacag cttgcctgcc attgcaggag ccgtttcccc gtcaggcagc ttcccagttg
    16861 caccacaatt tgcgcctgct gcagctcgct ggtcgctttg gcattgccta accaaagatc
    16921 ggcacacggc agcggctgcc cggtttgtga cagcacatcg ccaatcagcg gccagccaag
    16981 agtttgcgcc cacagggcaa cttttttgcc ctcttccgca ctcatgcgcc cggcaaccac
    17041 cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt ttttcacttt ccagacgagg
    17101 cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca cccagacgct gttgccagct
    17161 aagcccggta tcgtccattt cgccatacag cggttcagca aacgggcagt tgatatggac
    17221 tccccccaca tgaagcgtac cgagagcgtg gtcgatggta gaaaccagcc aacgtgcggg
    17281 gatatcctgg gtcgggcgcg gcaatgaaat actgtgcgtg gggtgagagc cgaacattcc
    17341 cggctggcga attgcctgat tcgcgccgca gtcaattagc tccggcgggc gatcggcggt
    17401 taagagaatc agtttttctc cggttaaccc ggcttcaatc agtgccggat agagatttgc
    17461 caccgccgtg ccggaggtca caatcaccgc caccggctgc ttgctgactt tcgccagccc
    17521 cagcgccaga tgccccaacc cacgctcatc gaaatgggtg tggtgaatga atgcggaatt
    17581 ctccgccgcc gctaacgtta acggtgtaga acgcgagcct ggggcgatac agatgtgtct
    17641 gacgccgtga cgcgttaatg cttccagaat gaccgccgcc cagcgtcggt taaatgcgct
    17701 tactgacatg agtttgtccg gtatcaatat tgcggctaag tataaggagc tacaaaaata
    17761 gattattgat atgaatcggt aatgatgcga ctcattacta ttccatttgt aataaagtac
    17821 gcagcactgc cgctttgttg tcgatttcct gccactcttg ctcggggtcg gaaccacgga
    17881 caatgcccgc gccagcatat aatcgcacga cattgccgct aatttttgct gagcgcaggg
    17941 aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc agaaccggcg taccattctc
    18001 gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag atcgcgcggt aagccagcaa
    18061 ctgctgccgt tggctgcaac tgatgtaaac agatcacatc atccgctttg ttgagtgaag
    18121 tccagataca gcggcgaaga tgctgcactt tacgcagacg cagtacctgc ggcggtaaaa
    18181 catccagcgt ctgggtatcg gcctgtaatc gttggcagat atcttccacc accagcatgt
    18241 tctcgcgctg gtttttatca tccgccatca gccactctcc taactgctgc gcctgcttat
    18301 catcaggatg atttgctact gttcccgcca gcgcttcagt acgcagcgct ttgtcacgcc
    18361 gccgccataa ccgttccggt gaagagccaa gaaaagcatt ttcgccatca aaggccatgt
    18421 aaaaatggta gcaattcaga ttcagtcgac gactggcagc catcatcgcc gccgcgttga
    18481 ccggacttgc gaaatgcagg tcagttgccc gagcgagcac cactttgtcg agctcacctt
    18541 cggcgatggt tttcgttgcc agttcgatta attgcgtcca gcccgttttg tccggccagt
    18601 gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg cttgatactc accagtgtgg
    18661 cgataaattc ttttgcctga atcgcatcgt gctgaaggga gctttcgctg aatagcgtca
    18721 gccgcagcgt ggctttaccg ccacagcgtc gccattccag gcggggtaaa agtaaattgc
    18781 cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa gtcggcgtgt tccgggtgct
    18841 ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg ggtaatcgcg cccagggcgg
    18901 cagcttcttc atcaccatta cgttgttgcc agtagaattg cgggtacgtt tgctgactgg
    18961 ccagccagct caaggcatca aaagcgtctt tgagagggaa aggaatatcg ataacccgaa
    19021 tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag tagattttcc agcgccgtag
    19081 taagtgattg cacgcggacc tctccctgtt aaaagcctca cattatacgg ggtactacaa
    19141 aaaaaatgca gtaccccggt gtagggaggt tttaacggcg tgccagcaac agtcctagta
    19201 ccagcccgac ggccgcgccc acaccaattc cttgccaggg tttttcgtgg acgtagtcat
    19261 cagcacgata aacagcctgc ttcgcccgat agtaataact gtctgaagcc tggctaaccc
    19321 gttttttcac atcatccagc gctttttctg cacgcgcttt cagctcaaca tatttctgat
    19381 cggcgggatc gccagaggag cggagcacct cttccagtgt ttcactaagc agcgtcaggt
    19441 cgtcatcgat acgtgtatca ccaaactgat tagacattct cgttctccat ttgcgtaaaa
    19501 cctgtcacta actatagaca agagttgttg attacgcctg aattacctcg cgtgccatcc
    19561 caatgtgtgg gataccatct tcttcataga cctccgtcac agggataaaa ccgaaactct
    19621 ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac aggcttatca ggccagtgat
    19681 gcgtacatgt ttcgagtgtt ttactcatca gctgctgccc cactttttca ccacgcaacg
    19741 cttcactgac aatcacccga cctataacga ccggctcaag atcatcatca cttttcagaa
    19801 tcctcgcata cgccaccagt tcatcatttt tccaaccgag aatatggcga ttatcccccg
    19861 tcaaatcatc accatcgata tcctgataag ggcaattctg ctcgacaaca aacaccgcgc
    19921 aacgcagttg taataaggca taaagctggg aaaccgataa ttcagagtga tgcagatctt
    19981 gccattcaat cataatgctc tccttttaga tgtatgcctt atactagccg gaatattttt
    20041 tgaaacgtga tgaactcatg gaattaattt ttttaggtac ttcagccggt gtgccaaccc
    20101 gcacgcgcaa tgtcacggca atattgctta acctgcaaca tccgacccag agcggacttt
    20161 ggttgtttga ctgcggtgaa ggcacccagc atcagctact gcataccgcc tttaaccctg
    20221 gaaaactgga caagattttt atcagtcacc ttcatggcga tcatcttttt ggtttacccg
    20281 gcttgctgtg cagtcgttct atgtcaggca ttatccaacc cttaacgatt tatggtccgc
    20341 aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag cggctcatgg accgattatc
    20401 cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga tgatggcctg cgcaaagtaa
    20461 ccgcttatcc gctggaacac ccactggaat gttatggcta tcgtattgaa gaacatgata
    20521 aaccgggtgc attaaatgcc caggcattaa aagctgctgg cgtgccgcct ggcccactgt
    20581 ttcaggaatt aaaagcgggc aaaacaatca cgctggaaga tggaaggcag attaacggcg
    20641 cagattactt agctgctcca gtgccaggta aagcgctcgc tattttcggc gataccggcc
    20701 cctgcgatgc cgcacttgac ctggctaaag gtgtcgatgt catggtgcac gaagcgacgc
    20761 tggatataac catggaagcc aaagccaata gtcgcggcca tagctctaca cgccaggctg
    20821 cgacactagc ccgtgaggct ggagtcggca agctaatcat tacccacgtc agctcgcgct
    20881 atgatgacaa aggttgtcaa cacctgttac gtgaatgcag gtcaattttc ccggcgactg
    20941 aactggcgaa tgatttcacc gtgtttaacg tttaactctg cttattatta aaaacagggt
    21001 gaaacttgcc ctgttatagc aacccgcgct acatatatcc acccacacag gcatattgag
    21061 cgatttttga tatttattca tctccttacg cactatattc agagcaataa gttgcctctt
    21121 ttcatcttga ttcagcacaa cctttgcaac taaaaggagc ccatgatggt tacagttgtc
    21181 agcaattatt gtcaattatc tcaaacccaa ctcagtcaga catttgcaga aaaatttact
    21241 gtgaccgagg aattactgca gtctttaaaa aaaacagcgt tatccggaga tgaagaaagt
    21301 attgagttac tgcataatat tgcgttaggt tatgatgaat ttggaaaaaa agctgaagat
    21361 attctttacc atattgttag aaacccaaca aatgagaccc tatcgattat ccgacttatt
    21421 aaaaattcct gtttaaaatt atataatttg gcacataccg caaccaactc ccaccttaaa
    21481 ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc cccttcgaaa
    21541 ttaatgacaa ttatcggtaa tgaaattcct cttatatctg aaaaacagtc gctttcaaag
    21601 gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg ggataaaaat
    21661 cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc taatgcaaaa
    21721 aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag aactcacctg
    21781 gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga agtccagtta
    21841 ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat agtaaataaa
    21901 aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca ctaggccatg
    21961 ataaagaaaa ttcacttaag cgtgtcatta atagttttaa ttctgaactc atgggagaaa
    22021 tgtcgaataa taatataaaa gttcatttaa atgaatcaga gataatattt ttacatgccg
    22081 atcttcagca atacttaagc caaagttgcg gtgcgtttgt gtgcatggca gcccaggaag
    22141 tgattgaaca aagggaaagc aattctgaca gcgcccccta tacattatta aaaaactatg
    22201 ctgacagatt taaaaaatat tcagcagaag agcagtacga aattgatttt caacatcgac
    22261 tggtaaacag aaattgttat ttagataaat atggcgatgc acgaatcaat gcttcttata
    22321 cacaattaga aataaaacac tcacaaccca aaaatagagc atccggcaaa agagtgagtt
    22381 aacatcattg ttaatcaatt cgaccagtca gtaaatatgc tgactggtcg aacataattt
    22441 gctaaaaagt catcactgac tgatgtcagt cccaccatct gccagttcaa tctacgggta
    22501 tttccagcgt atcgcggcac gcgattgctg gtctgttgat gtagtggatt gtagttgctg
    22561 acttttctta ccctcagatt tctgccccac ttgtcaattc aatctgccaa taaataacat
    22621 aattatattt ttattcctca cagaaattcc tttttaaaaa tagcgaaccc actgtaaaaa
    22681 acactttttg tatacctcta ccaagcgtaa taaaataaac gctcataatt cattataaat
    22741 aataatgaaa taacgccttt tattgataat ttacatatga aaaaaatttt ccttttgtta
    22801 tattatttct gttttcactc cctctgatta tttttaccaa ccctgggtca ctgcattatc
    22861 gccaacgccc cgacacgtaa gtcctgagca attagaaaaa aca
//
LOCUS       DADLSB010000052        22770 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.052, whole genome shotgun sequence.
ACCESSION   DADLSB010000052 DADLSB010000000
VERSION     DADLSB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.052"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            262..645
                     /gene="secE"
                     /locus_tag="JN331_001986"
     CDS             262..645
                     /gene="secE"
                     /locus_tag="JN331_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY7260138.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            647..1192
                     /gene="nusG"
                     /locus_tag="JN331_001987"
     CDS             647..1192
                     /gene="nusG"
                     /locus_tag="JN331_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY7260139.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1351..1779
                     /gene="rplK"
                     /locus_tag="JN331_001988"
     CDS             1351..1779
                     /gene="rplK"
                     /locus_tag="JN331_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY7260140.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1783..2487
                     /gene="rplA"
                     /locus_tag="JN331_001989"
     CDS             1783..2487
                     /gene="rplA"
                     /locus_tag="JN331_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY7260141.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2900..3397
                     /gene="rplJ"
                     /locus_tag="JN331_001990"
     CDS             2900..3397
                     /gene="rplJ"
                     /locus_tag="JN331_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY7260142.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3464..3829
                     /gene="rplL"
                     /locus_tag="JN331_001991"
     CDS             3464..3829
                     /gene="rplL"
                     /locus_tag="JN331_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY7260143.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4149..8177
                     /gene="rpoB"
                     /locus_tag="JN331_001992"
     CDS             4149..8177
                     /gene="rpoB"
                     /locus_tag="JN331_001992"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY7260144.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8254..12477
                     /gene="rpoC"
                     /locus_tag="JN331_001993"
     CDS             8254..12477
                     /gene="rpoC"
                     /locus_tag="JN331_001993"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY7260145.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12719..13852)
                     /gene="thiH"
                     /locus_tag="JN331_001994"
     CDS             complement(12719..13852)
                     /gene="thiH"
                     /locus_tag="JN331_001994"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY7260146.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13849..14694)
                     /gene="thiG"
                     /locus_tag="JN331_001995"
     CDS             complement(13849..14694)
                     /gene="thiG"
                     /locus_tag="JN331_001995"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY7260147.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIADKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14621..14821)
                     /gene="thiS"
                     /locus_tag="JN331_001996"
     CDS             complement(14621..14821)
                     /gene="thiS"
                     /locus_tag="JN331_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY7260148.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14805..15560)
                     /gene="thiF"
                     /locus_tag="JN331_001997"
     CDS             complement(14805..15560)
                     /gene="thiF"
                     /locus_tag="JN331_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY7260149.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15553..16188)
                     /gene="thiE"
                     /locus_tag="JN331_001998"
     CDS             complement(15553..16188)
                     /gene="thiE"
                     /locus_tag="JN331_001998"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY7260150.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16188..18083)
                     /gene="thiC"
                     /locus_tag="JN331_001999"
     CDS             complement(16188..18083)
                     /gene="thiC"
                     /locus_tag="JN331_001999"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY7260151.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18164..18264)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18317..18793)
                     /gene="rsd"
                     /locus_tag="JN331_002000"
     CDS             complement(18317..18793)
                     /gene="rsd"
                     /locus_tag="JN331_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY7260152.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18888..19661
                     /gene="nudC"
                     /locus_tag="JN331_002001"
     CDS             18888..19661
                     /gene="nudC"
                     /locus_tag="JN331_002001"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY7260153.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19701..20765
                     /gene="hemE"
                     /locus_tag="JN331_002002"
     CDS             19701..20765
                     /gene="hemE"
                     /locus_tag="JN331_002002"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY7260154.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20922..21593
                     /gene="nfi"
                     /locus_tag="JN331_002003"
     CDS             20922..21593
                     /gene="nfi"
                     /locus_tag="JN331_002003"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY7260155.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21636..22226
                     /locus_tag="JN331_002004"
     CDS             21636..22226
                     /locus_tag="JN331_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY7260156.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22413..22685
                     /gene="hupA"
                     /locus_tag="JN331_002005"
     CDS             22413..22685
                     /gene="hupA"
                     /locus_tag="JN331_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY7260157.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22698..>22770
                     /locus_tag="JN331_002006"
     CDS             22698..>22770
                     /locus_tag="JN331_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY7260158.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAG"
BASE COUNT         5421 a         5874 c         6233 g         5242 t
ORIGIN      
        1 gcgcgggcgt tgttgctaaa gttctgagct aattgccgat aacatttgac gcaatgcgca
       61 ctaaaagggc atcatttgat gccctttttg cacgctttcg taccagaacc tggctcatca
      121 gtgattttct ttgtcataat cattgctgag acaggctctg ttgagggcgt ataatccgaa
      181 aagctaatac gcgtttcgat ttggtttgcc tcgcgatcgc ggggtgaaaa tgtttgtaga
      241 aaacttctga caggttggtt tatgagtgcg aataccgaag ctcaaggaag cgggcgcggc
      301 ctggaagcga tgaagtgggt cgttgtggtg gcattgctcc tggtggcgat tgtcggcaac
      361 tacctttatc gcgacattat gctgccgctg cgtgcgctgg ccgtagtaat tctgattgct
      421 gcagcgggtg gtgtcgcgct gttaacgaca aaaggtaaag ctaccgttgc ttttgcccgt
      481 gaagcgcgta ccgaagtccg taaggtcatt tggccgactc gccaggaaac attgcacacc
      541 acgctgattg tggctgcggt taccgcagta atgtcactga tcctgtgggg actggatggt
      601 attctggttc gcctggtatc ctttatcact ggcctgaggt tctgagatgt ctgaagctcc
      661 taaaaagcgc tggtacgtcg ttcaggcgtt ttccggtttt gaaggccgcg tagcaacgtc
      721 gctgcgtgag catatcaaat tacacaacat ggaagatttg tttggtgaag tcatggtacc
      781 aaccgaagaa gtggttgaaa tccgtggcgg tcagcgtcgc aaaagcgaac gtaaattctt
      841 ccctggctac gtcctcgttc agatggtgat gaacgacgcg agctggcact tggtgcgcag
      901 cgtaccgcgt gtgatgggct tcatcggcgg tacttccgat cgtcctgcgc caatcagcga
      961 taaagaagtc gatgcgatta tgaaccgcct gcagcaggtt ggtgataagc cgcgtccgaa
     1021 aacgctgttt gaaccgggtg aaatggtccg tgttaatgat ggtccgttcg ctgacttcaa
     1081 cggtgttgtt gaagaagtgg attacgagaa atctcgtctg aaagtgtctg tttctatctt
     1141 cggtcgtgcg accccggtag agctggactt cagccaggtt gaaaaagcct aacccagcga
     1201 tcaaaaaagc ggcgatttaa tcgttgcaca aggcgtgaga ttggaataca atttcgcgcc
     1261 ttttgttttt atgggccttg cccgtaaaac gattttttat atcacgggga gcctctcaga
     1321 ggcgttatta cccaacttga ggaatttata atggctaaga aagtacaagc ctatgtcaag
     1381 ctgcaggttg cagctggtat ggctaacccg agtccgccag taggtccggc tctgggtcag
     1441 cagggcgtaa acatcatgga attctgcaaa gcgttcaacg caaaaactga ttccatcgaa
     1501 aaaggtctgc cgattccggt agtaatcacc gtttacgctg accgttcttt cactttcgtt
     1561 accaagaccc cgccggcagc agttctgctg aaaaaagcgg ctggtatcaa gtctggttcc
     1621 ggtaagccga acaaagacaa agtgggtaaa atttcccgcg ctcagctgca ggaaatcgcg
     1681 cagaccaaag ctgccgacat gactggtgcc gacattgaag caatgactcg ctccatcgaa
     1741 ggtactgcac gttccatggg cctggtagtg gaggactaag aaatggctaa actgaccaag
     1801 cgcatgcgtg ttatccgcga gaaagttgat gcaaccaaac agtacgacat caacgaagct
     1861 atcgcactgc tgaaagagct ggcgactgct aaattcgtag aaagcgtgga cgtagctgtt
     1921 aacctcggca tcgacgctcg taaatctgac cagaacgtac gtggtgcaac tgtactgccg
     1981 cacggtactg gccgttccgt tcgcgtagcc gtatttaccc aaggtgcaaa cgctgaagct
     2041 gctaaagctg caggcgcaga actggtaggt atggaagatc tggctgacca gatcaagaaa
     2101 ggcgaaatga actttgacgt tgttattgct tctccggatg caatgcgcgt tgttggccag
     2161 ctgggccagg ttctgggtcc gcgcggcctg atgccaaacc cgaaagtggg tactgtaaca
     2221 ccgaacgttg ctgaagcggt taaaaacgct aaagctggcc aggttcgtta ccgtaacgac
     2281 aaaaacggca tcatccacac caccatcggt aaagtggact ttgacgctga caaactgaaa
     2341 gaaaacctgg aagctctgct ggttgcgctg aaaaaagcaa aaccgactca ggcgaaaggc
     2401 gtgtacatca agaaagttag catctccacc accatgggtg caggtgttgc agttgaccag
     2461 gctggcctga gcgcttctgt aaactaatgc ctttacgtgg gaggtgattt tgtctacaat
     2521 cttaccccca cgtataatgc ttaatgcaga cgtatatccg agatattcgg gttgtggcaa
     2581 ggcggcaact gagtgagtcg ccagtcacat agctaactat gtgactggtg cgaatgaagg
     2641 aagccaacgc cgccacaagt tgaatagcga cggatagaaa agatttgttc gttggagcct
     2701 ggcctatcca ggcctccgtc gaagaccgca ggagtttcgc aagaaactta atcccctgcg
     2761 tagacggtga cagaacgcta agattattct tttatattct ggcttgtttc tgctcaccgt
     2821 aattaagacg ctctctccgt ttggaggagt gaagtgagtt ccagagattt tctctggcaa
     2881 acatccagga gcaaagctaa tggctttaaa tcttcaagac aaacaagcga ttgttgctga
     2941 agtcagcgaa gtagccaaag gcgcgctgtc tgcagtagtt gcggattccc gtggcgtaac
     3001 tgtagataaa atgactgaac tgcgtaaagc aggtcgcgaa gctggcgtat acatgcgtgt
     3061 tgttcgtaac accctgctgc gccgtgctgt tgaaggtact ccgttcgagt gcctgaaaga
     3121 cgcgtttgtt ggtccgaccc tgattgcata ctctatggaa cacccgggcg ctgctgctcg
     3181 tctgttcaaa gagttcgcga aagcgaatgc aaaatttgag gtcaaagccg ctgcctttga
     3241 aggtgagctg atcccggcgt ctcagatcga ccgcctggca actctgccga cctacgaaga
     3301 agcaattgca cgcctgatgg caaccatgaa agaagcttcg gctggcaaac tggttcgtac
     3361 tctggctgct gtacgcgatg cgaaagaagc tgcttaatcg cagttatctt tttaacgcat
     3421 tcgcttacgt ataaacttat tctgatattc aggaacaatt taaatgtcta tcactaaaga
     3481 tcaaatcatt gaagcagttg cagctatgtc tgtaatggac gttgtagaac tgatctctgc
     3541 aatggaagaa aaattcggtg tttccgctgc tgctgctgta gctgtagctg ctggcccggt
     3601 tgaagctgct gaagaaaaaa ctgaattcga cgtaattctg aaagctgctg gcgctaacaa
     3661 agttgctgtt atcaaagcag tacgtggcgc aactggcctg ggtctgaaag aagctaaaga
     3721 cctggtagaa tctgcaccgg ctgctctgaa agaaggcgtg agcaaagacg acgcagaagc
     3781 actgaaaaaa gctctggaag aagctggcgc tgaagttgaa gttaaataag ccaacccttc
     3841 cggttgcagc ctgagaaatc aggctgatgg ctggtgactt tttagtcacc agcctttttg
     3901 cgctgtaagg cgccagtagc gtttcacact gtttgactac tgctgtgcct ttcaatgctt
     3961 gtttctatcg acgacttaat atactgcgac aggacgtccg ttctgtgtaa atcgcaatga
     4021 aatggtttaa gcgtgatagc aacaggcatt gcggaaagtg ttccattttc cggtcaacaa
     4081 aatagtgttg cacaaactgt ccgctcaatg gacagatggg tcgacttgtc agcgagctga
     4141 ggaaccctat ggtttactcc tataccgaga aaaaacgtat tcgtaaggat tttggtaaac
     4201 gtccacaagt tctggatgta ccttatctcc tttctatcca gcttgactcg tttcagaaat
     4261 ttatcgagca agatcctgaa gggcagtatg gtctggaagc tgctttccgt tccgtattcc
     4321 cgattcagag ctacagcggt aattccgagc tgcaatacgt cagctaccgc cttggcgaac
     4381 cggtgtttga cgtccaggaa tgtcaaatcc gtggcgtgac ctattccgca ccgctgcgcg
     4441 ttaaactgcg tctggtgatc tatgagcgcg aagcgccgga aggcaccgta aaagacatta
     4501 aagaacaaga agtctacatg ggcgaaattc cgctcatgac agacaacggt acctttgtta
     4561 tcaacggtac tgagcgtgtt atcgtttccc agctgcaccg tagtccgggc gtcttctttg
     4621 actccgacaa aggtaaaacc cactcttcgg gtaaagtgct gtataacgcg cgcatcatcc
     4681 cttaccgtgg ttcctggctg gacttcgaat tcgatccgaa ggacaacctg ttcgtacgta
     4741 tcgaccgtcg ccgtaaactg cctgcgacca tcattctgcg cgccctgaac tacaccacag
     4801 agcagatcct cgacctgttc tttgaaaaag ttatctttga aatccgtgat aacaagctgc
     4861 agatggaact ggtgccggaa cgcctgcgtg gtgaaaccgc atcttttgac atcgaagcta
     4921 acggtaaagt gtacgtagaa aaaggccgcc gtatcactgc gcgccacatt cgccagctgg
     4981 aaaaagacga cgtcaaactg atcgaagtcc cggttgagta catcgcaggt aaagtggttg
     5041 ctaaagacta tattgatgag tctaccggcg agctgatctg cgcagcgaac atggagctga
     5101 gcctggatct gctggctaag ctgagccagt ctggtcacaa gcgtatcgaa acgctgttca
     5161 ccaacgatct ggatcacggc ccatatatct ctgaaacctt acgtgtcgac ccaactaacg
     5221 accgtctgag cgcactggta gaaatctacc gcatgatgcg ccctggcgag ccgccgactc
     5281 gtgaagcagc tgaaagcctg ttcgagaacc tgttcttctc cgaagaccgt tatgacttgt
     5341 ctgcggttgg tcgtatgaag ttcaaccgtt ctctgctgcg cgaagaaatc gaaggttccg
     5401 gtattctgag caaagacgac atcattgatg ttatgaaaaa gctcatcgat atccgtaacg
     5461 gtaaaggcga agtcgatgat atcgaccacc tcggcaaccg tcgtatccgt tccgttggcg
     5521 aaatggcgga aaaccagttc cgcgttggcc tggtacgtgt agagcgtgcg gtgaaagagc
     5581 gtctgtctct gggcgatctg gataccctga tgcctcagga tatgatcaac gccaagccga
     5641 tttccgcagc agtgaaagag ttcttcggtt ccagccagct gtctcagttt atggaccaga
     5701 acaacccgct gtctgagatt acgcacaaac gtcgtatctc cgcactcggc ccaggcggtc
     5761 tgacccgtga acgtgcaggc ttcgaagttc gagacgtaca cccgactcac tacggtcgcg
     5821 tatgtccaat cgaaacccct gaaggtccga acatcggtct gatcaactct ctgtccgtgt
     5881 acgcacagac taacgaatac ggcttccttg agactccgta tcgtaaagtg accgacggtg
     5941 ttgtaactga cgaaattcac tacctgtctg ctatcgaaga aggcaactac gttatcgccc
     6001 aggcgaactc caacctggat gaagaaggcc acttcgtaga agacctggta acttgccgta
     6061 gcaaaggcga atccagcttg ttcagccgcg accaggttga ctacatggac gtatccaccc
     6121 agcaggtggt atccgtcggt gcgtccctga tcccgttcct ggaacacgat gacgccaacc
     6181 gtgcattgat gggtgcgaac atgcaacgtc aggccgttcc gactctgcgc gctgataagc
     6241 cgctggttgg tactggtatg gaacgtgctg ttgccgttga ctccggtgta actgcggtag
     6301 ctaaacgtgg tggtgtcgtt cagtacgtgg atgcttcccg tatcgttatc aaagttaacg
     6361 aagacgagat gtatccgggt gaagcaggta tcgacatcta caacctgacc aaatacaccc
     6421 gttctaacca gaacacctgt atcaaccaga tgccgtgtgt gtctctgggt gaaccggttg
     6481 aacgtggcga cgtgctggca gacggtccgt ccaccgacct cggtgaactg gcgcttggtc
     6541 agaacatgcg cgtagcgttc atgccgtgga atggttacaa cttcgaagac tccatcctcg
     6601 tatccgagcg tgttgttcag gaagaccgtt tcaccaccat ccacattcag gaactggcgt
     6661 gtgtgtcccg tgacaccaag ctgggtccgg aagagatcac cgctgacatc ccgaacgtgg
     6721 gtgaagctgc gctctccaaa ctggatgaat ccggtatcgt ttacattggt gcggaagtga
     6781 ccggtggcga cattctggtt ggtaaggtaa cgccgaaagg tgaaactcag ctgaccccag
     6841 aagaaaaact gctgcgtgcg atcttcggtg agaaagcctc tgacgttaaa gactcttctc
     6901 tgcgcgtacc aaacggtgta tccggtacgg ttatcgacgt tcaggtcttt actcgcgatg
     6961 gcgtagaaaa agacaaacgt gcgctggaaa tcgaagaaat gcagctcaaa caggcgaaga
     7021 aagacctgtc tgaagaactg cagatcctcg aagcgggtct gttcagccgt atccgtgctg
     7081 tgctggtagc cggtggcgtt gaagctgaga agctcgacaa actgccgcgc gatcgctggc
     7141 tggagctggg cctgacagac gaagagaaac aaaatcagct ggaacagctg gctgagcagt
     7201 atgacgaact gaaacacgag ttcgagaaga aactcgaagc gaaacgccgc aaaatcaccc
     7261 agggcgacga tctggcaccg ggcgtgctga agattgttaa ggtatatctg gcggttaaac
     7321 gccgtatcca gcctggtgac aagatggcag gtcgtcacgg taacaagggt gtaatttcta
     7381 agatcaaccc gatcgaagat atgccttacg atgaaaacgg tacgccggta gacatcgtac
     7441 tgaacccgct gggcgtaccg tctcgtatga acatcggtca gatcctcgaa acccacctgg
     7501 gtatggctgc gaaaggtatc ggcgacaaga tcaacgccat gctgaaacag cagcaagaag
     7561 tcgcgaaact gcgcgaattc atccagcgtg cgtacgatct gggcgctgac gttcgtcaga
     7621 aagttgacct gagtaccttc agcgatgaag aagttatgcg tctggctgaa aacctgcgca
     7681 aaggtatgcc aatcgcaacg ccggtgttcg acggtgcgaa agaagcagaa attaaagagc
     7741 tgctgaaact tggcgacctg ccgacttccg gtcagatccg cctgtacgat ggtcgcactg
     7801 gtgaacagtt cgagcgtccg gtaaccgttg gttacatgta catgctgaaa ctgaaccacc
     7861 tggtcgacga caagatgcac gcgcgttcca ccggttctta cagcctggtt actcagcagc
     7921 cgctgggtgg taaggcacag ttcggtggtc agcgtttcgg ggagatggaa gtgtgggcgc
     7981 tggaagcata cggcgcagca tacaccctgc aggaaatgct cactgttaag tctgatgacg
     8041 tgaacggtcg taccaagatg tataaaaaca tcgtggacgg caaccatcag atggagccgg
     8101 gcatgccaga atccttcaac gtattgttga aagagattcg ttcgctgggt atcaacatcg
     8161 aactggaaga cgagtaattc tcgctcaaac aggtcactgc tgtcggggta aaacccggca
     8221 gcggattgtg ctaactccga cgggagcaaa tccgtgaaag atttattaaa gtttctgaaa
     8281 gcgcagacta aaaccgaaga gtttgatgcg atcaaaattg ctctggcttc gccagacatg
     8341 atccgttcat ggtctttcgg tgaagttaaa aagccggaaa ccatcaacta ccgtacgttc
     8401 aaaccagaac gtgacggcct tttctgcgcc cgtatctttg ggccggtaaa agattacgag
     8461 tgcctgtgcg gtaagtacaa gcgcctgaaa caccgtggcg tcatctgtga gaagtgcggc
     8521 gttgaagtga cccagactaa agtacgccgt gagcgtatgg gccacatcga actggcttcc
     8581 ccgactgcgc acatctggtt cctgaaatcg ctgccgtccc gtatcggtct gctgctcgat
     8641 atgccgctgc gcgatatcga acgcgtactg tactttgaat cctatgtggt tatcgaaggc
     8701 ggtatgacca acctggaacg tcagcagatc ctgactgaag agcagtatct ggacgcgctg
     8761 gaagagttcg gtgacgaatt cgacgcgaag atgggtgcgg aagcaatcca ggctctgctg
     8821 aagagcatgg atctggagca agagtgcgaa cagctgcgtg aagagctgaa cgaaaccaac
     8881 tccgaaacca agcgtaaaaa gctgaccaag cgtatcaaac tgctggaagc gttcgttcag
     8941 tctggtaaca aaccagagtg gatgatcctg accgttctgc cggtactgcc gccagatctg
     9001 cgtccgctgg ttccgctgga tggtggtcgt ttcgcgactt ctgacctgaa cgatctgtat
     9061 cgtcgcgtca ttaaccgtaa caaccgtctg aaacgtctgc tggatctggc tgcgccggac
     9121 atcatcgtac gtaacgaaaa acgtatgctg caggaagcgg tagacgccct gctggataac
     9181 ggtcgtcgcg gtcgtgcgat caccggttct aacaagcgtc ctctgaaatc tttggccgac
     9241 atgatcaaag gtaaacaggg tcgtttccgt cagaacctgc tcggtaagcg tgttgactac
     9301 tccggtcgtt ctgtaatcac cgtaggtcca tacctgcgtc tgcatcagtg cggtctgccg
     9361 aagaaaatgg cgctggagct gttcaaaccg ttcatctacg gcaagctgga actgcgtggt
     9421 cttgctacca ccattaaagc tgcgaagaaa atggttgagc gcgaagaagc tgtcgtttgg
     9481 gatatcctgg acgaagttat ccgcgaacac ccggtactgc tgaaccgtgc accgactctg
     9541 caccgtctgg gtatccaggc atttgaaccg gtactgatcg aaggtaaagc tatccagctg
     9601 cacccgctgg tttgtgcggc atataacgcc gacttcgatg gtgaccagat ggctgttcac
     9661 gtaccgctga cgctggaagc ccagctggaa gcgcgtgcgc tgatgatgtc taccaacaac
     9721 atcctgtccc cggcgaacgg cgaaccaatc atcgttccgt ctcaggacgt tgtactgggt
     9781 ctgtactaca tgacccgtga ctgtgttaac gccaaaggcg aaggcatggt gctgactggc
     9841 ccgaaagaag cagaacgtct gtatcgctct ggtctggctt ctctgcatgc gcgcgttaaa
     9901 gtgcgtatca ccgagtatga aaaagatgct aacggtgaat tagtagcgaa aaccagcctg
     9961 aaagacacga ctgttggccg tgccattctg tggatgattg taccgaaagg tctgccttac
    10021 tccatcgtca accaggcgct gggtaaaaaa gcaatctcca aaatgctgaa cacctgctac
    10081 cgcattctcg gtctgaaacc gaccgttatt tttgcggacc agatcatgta caccggcttc
    10141 gcctatgcag cgcgttctgg tgcatctgtt ggcatcgatg acatggtcat cccggagaag
    10201 aaacacgaaa tcatctccga ggcagaagca gaagttgctg aaattcagga gcagttccag
    10261 tctggtctgg taactgcggg cgaacgctac aacaaagtta tcgatatctg ggctgcggcg
    10321 aacgatcgtg tatccaaagc gatgatggat aacctgcaaa ctgaaaccgt tattaaccgt
    10381 gacggtcagg aagagaagca ggtttccttc aacagcatct acatgatggc cgactccggt
    10441 gcgcgtggtt ctgcggcaca gattcgtcag cttgctggta tgcgtggtct gatggcgaag
    10501 ccggatggct ccatcatcga aacgccaatc accgcgaact tccgtgaagg tctgaacgta
    10561 ctccagtact tcatctccac ccacggtgct cgtaaaggtc tggcggatac cgcactgaaa
    10621 actgcgaact ccggttacct gactcgtcgt ctggttgacg tggcgcagga cctagtggtt
    10681 accgaagacg attgtggtac ccatgaaggt atcatgatga ctccggttat cgagggtggt
    10741 gacgttaaag agccgctgcg cgatcgcgta ctgggtcgtg taactgctga agacgttctg
    10801 aagccgggta ctgctgatat cctcgttccg cgcaacacgc tgctgcacga acagtggtgt
    10861 gacctgctgg aagagaactc tgtcgacgcg gtaaaagtac gttctgttgt atcttgtgac
    10921 accgactttg gtgtatgtgc gcactgctac ggtcgtgacc tggcgcgtgg ccacatcatc
    10981 aacaaaggtg aagcaatcgg tgttatcgcg gcacagtcca tcggtgaacc gggtacacag
    11041 ctgaccatgc gtacgttcca catcggtggt gcggcatctc gtgcggctgc tgaatccagc
    11101 atccaggtga aaaacaaagg tagcatcaag ctcagcaacg tgaagtcggt tgtgaactcc
    11161 agcggtaaac tggttatcac ttcccgtaac accgaactga aactgatcga cgaattcggt
    11221 cgtaccaaag aaagctacaa agtgccttac ggtgcggtac tggcgaaagg cgatggcgaa
    11281 caggttgctg gcggcgaaac cgttgcaaac tgggacccgc acaccatgcc ggttatcacc
    11341 gaagtaagcg gttttgtacg ctttactgac atgatcgacg gccagaccat tactcgtcag
    11401 accgacgaac tgaccggtct gtcttcgctg gtggttctgg attccgcaga acgtaccgca
    11461 ggtggtaaag atctgcgtcc ggcactgaaa atcgttgatg ctcagggtaa cgacgttctg
    11521 atcccaggta ccgatatgcc tgcgcagtac ttcctgccgg gtaaagcgat tgttcagctg
    11581 gaagatggcg tacagatcag ctctggtgac accctggcgc gtattccgca ggaatccggc
    11641 ggtaccaagg acatcaccgg tggtctgccg cgcgttgcgg acctgttcga agctcgtcgt
    11701 ccgaaagagc cggcaatcct ggctgaaatc agcggtatcg tttccttcgg taaagaaacc
    11761 aaaggtaaac gtcgtctggt tatcaccccg gtagacggta gcgatccgta cgaagagatg
    11821 attccgaaat ggcgtcagct caacgtgttc gaaggtgaac gtgtagaacg tggtgacgta
    11881 atttccgacg gtccggaagc gccgcacgac attctgcgtc tgcgtggtgt tcatgctgtg
    11941 actcgttaca tcgttaacga agtacaggac gtataccgtc tgcagggcgt taagattaac
    12001 gataaacaca tcgaagttat cgttcgtcag atgctgcgta aagctaccat cgttaacgcg
    12061 ggcagctccg acttcctgga aggcgaacag gttgaatact ctcgcgtcaa gatcgcaaac
    12121 cgcgaactgg aagcgaacgg caaagtgggt gcaacttact cccgcgatct gctgggtatc
    12181 accaaagcgt ctctggcaac cgagtccttc atctccgcgg catcgttcca ggagaccact
    12241 cgcgtgctga ccgaagcagc cgttgcgggc aaacgcgacg aactgcgcgg cctgaaagag
    12301 aacgttatcg tgggtcgtct gatcccggca ggtaccggtt acgcgtacca ccaggatcgt
    12361 atgcgtcgcc gtgctgcggg tgaagctccg gctgcaccgc aggtgactgc agaagacgca
    12421 tctgccagcc tggcagaact gctgaacgca ggtctgggcg gttctgataa cgagtaatcg
    12481 ttaatccgca aataacgtaa aaacccgctt cggcgggttt ttttatgggt gttattcaga
    12541 gggcaaattc ttcacggaaa atattaagaa aggcagggcg aaacgaggaa gaagcttttc
    12601 gccctggaca acattcctgc tgacggcact accataaaaa aacacaacgc tttcaacaac
    12661 cttgcgtttt tcatcttccg tagcggctcg gaataaattt acgttcttac attccgtctc
    12721 atagtctttg cgaggcgcgt cccagatagc tgtcccagtc tttccatacc ggctgcaaac
    12781 cctgagcggt taacgcggca gcaaccgctt ccggtctgcg atcgtcgtgc ggtgagaact
    12841 gttccaactc ggggtgatta tcggcatagc cacctggctg cgttttcgag aaggcgctga
    12901 cgttattgat cgccagcgga ataacgcgat cgcgaaacca cggtgattcc cgcgtggaga
    12961 gtgacagttc aatctccggt gcaagcagtc ggaaggcgca gatggtttgc actaactggc
    13021 gttcatccat aatcgacgca ggctcaatgc cgccagtaca cgggcgcagg cgcggaaagg
    13081 agacagagta acggctttgc cagtaatgct gttgcagcca tagcaaatgt tcggcaacca
    13141 tatagcagtc aacgcgccag ttgtcggaaa ggccaattag cgcgccgagg cctatcttat
    13201 caatccccgc acgccccagc cgatccggcg tttccagccg ccagaagaag tcctgttttt
    13261 tgcctttcaa atgatggcgg gtataagtcg cctcgtgata tgtctcctga taaaccatca
    13321 ccccatccag accaagttgc tttaactcgg cgtattccgt ctccgccagc ggttgcactt
    13381 ccatttgtag tgaagagaac tgttcacgca gggcggggag atgacgacga aagtaatcca
    13441 tccccacttt cgcctgatgt tcaccagtga ctaatagcag atgttcaaag cccatctccc
    13501 gtatagcggc gctttccctg gcaatatccg cttcatccag cgttttgcgc ttgatgcgat
    13561 tactcatgga aaatccgcag tacgtgcagt cgttagcgca aagattggaa agataaagcg
    13621 ggacgtagaa actaactgtg ttgccaaatc gctgacgggt cagacgctgc gcctgttggg
    13681 ccagttgttc cagatagcca ctggcggcag gcgataacag cgccatcatg tcgtcgcggg
    13741 tgagttgcga ggcatttagc gcccgctcta cgtcagcagc cgttttgccg ttgatacgca
    13801 ggcggatgtc gtcccagtcc agttgtcgcc agcgattgct gaaggttttc atgccgatgc
    13861 ctccagaaat ccggtcagcg ggctggtggc atgagcaaaa taactgcggc tgcccggtcc
    13921 ggactgacgt gccagcaggc ctgcttctac cgccagacga aatgccttcg ccatgttgac
    13981 gggatcgtcc gcgacggcaa tcgccgtatt cactaacacc gcgtcggccc ccatttccag
    14041 cgcctgcgcg gcatggctgg gaacgccgat gccagcatcg acaaccaccg gaactgtggc
    14101 ctgctggata ataatctcca gcatggcacg ggtttccagt ccctgattcg agccaatcgg
    14161 cgcgccgagc ggcatcaccg ctgcacagcc gacttcttcc agacgtttac acaataccgg
    14221 atcggccccg cagtaaggca gcacgacaaa tccctgttgt accagcgttt cggcggcttt
    14281 cagggtttcg atgggatcgg gcaacagcca gcgggcgtca gggtgaatct ccaattttag
    14341 ccagtttgtg cctaacgctt cacgtgccag atgggcggcg aaaatggctt cttccgctgt
    14401 tttcgccccg gaagtattcg gcagcagggc cacacccgcc gcgataagcg gttcgaggat
    14461 agcgtcgttg tgctggcgca agtcgacacg tttcatcgcc agtgtcacca gctggctgcc
    14521 ggaagcgcgg atcgcctcca ccatcagttg tgaagaagcg aatttccctg tgccggtaaa
    14581 cagatgtgaa tcaaacgttt tgtccgcaat acgtaacatc tcaaccccct gcaataacct
    14641 gaaaaagcag gatctggtcg ccatcctgca cgatatgttg cgtccactgc tcacgcggga
    14701 cgatttgctg attaatcgcc agagccgcgc ccgcttgtcg ttggtcgagt tgctccagta
    14761 gttcgtgaac agtttgcccg gcggcgcact gcatcggttg atcgttaaac aggatctgca
    14821 ttgcttcctc cgcataccgg gcaaccactg gcgcggcgca acgccaggct gcgccactgg
    14881 ctcgacttac cgtcgaacag tcggagttct cccgcaggcg tctctatacc gcttaataac
    14941 ttaatggctt ccagtgcctg caaagtgccc atcaccccga ccaccgggcc aaccacgccc
    15001 gccgtgcggc agttgcgttc tggctgctgg ttatctggcc acaggcagcg gtaacacccc
    15061 tgctcccagg gcggtgtcag taccatcaac tgaccgccaa atccgaccgc gctggcggtg
    15121 ataagcggcg tgttgagtgc cacgcaggcg gcattaatct cctggcgagt cgccatattg
    15181 tcggtacagt cgagcaccac atcggcttgt gcaaccgcat ctttcaacgc ctcacccgtt
    15241 aaccgttgtt gtaatgctgt cagttgaatg tcgggattca actgtgtcag tcgctgttgg
    15301 ctgacctgcg atttcgggct gttgatatct tcagtggtaa agaggatttg tcgttgcaga
    15361 ttgcttaaat gcacatcgtc gtcatctgcc agtaccagcg tcccgacgcc agcccctgcc
    15421 aggtatagcg cagcaggtgt acccagcccg ccaagaccaa taatcagcac ctggctgtcg
    15481 agcagttttt gctgcccgtc aagagcgata tcgtcgagca gaatttggcg gctataacgc
    15541 ataaagtcac ggtcattcat cgccaactcc tgcaatttcc agcaactgtg ccgttgccaa
    15601 acgccagtct gcggcttgag taatggcgct gacgacggcg atactgccga cacccgttgc
    15661 tatcaccgca ggcgcgcgtg ccagactgat accgccaatc gccacggtgg gataatccgc
    15721 caggcgctca acatgccgtg ccaactgttc cagcccctgc ggtgcagaag gcatctgttt
    15781 ggtttgcgtc gggaacacat gtcccagcgc gatataagag gggcgtgctg ccagcgcgac
    15841 gtcgatttcc atatcgtcat gtgtcgaaac gcccagccgc aggcctgccg cgcggatggc
    15901 attgagatcg gtggcttgca aatcttcctg ccccaaatgg acgccatacg cctgatgctt
    15961 gatcgcgagc cgccagtaat cgttgataaa caatcgcgcg ttatagcggc ggcccagcgc
    16021 aattgccgcc acgacatcgg cttccacctc ttcatcgcgc cgatctttga tgcgtagctg
    16081 gagagtacgt acgcctgcat ccaacagacg ttcgatccac tgtacgctgt ccaccaccgg
    16141 gtacagtcct aaacgaaaag gtacaggagg aaaatcaggc tgatacatca cgcttcctcc
    16201 ttatgcaggt agatttctcc gcctctggca cggaagttct ccgacatatc cgccattccc
    16261 acttcaatgg tttgcgcggc ggcgtaatcg cgtacttcct ggctgatttt catcgagcag
    16321 aattttggcc cacacatgga gcaaaaatgg gcgactttgc cggactcttg gggcagggtt
    16381 tcatcgtgat aagcgcgggc ggtaaacggg tcgagggcca gattaaactg gtcttcccag
    16441 cgaaattcga agcgggcttt cgacatggcg ttatcgcgaa tttgcgcgcc cggatgccct
    16501 tttgccaggt cagcagcgtg ggcggcaatc ttataagtga taagcccctg cttaacatct
    16561 tctttattgg gcagacccag atgctctttt ggcgttacgt aacagagcat cgcgcagcca
    16621 aaccagccaa tcatcgccgc accaatcccc gacgtgaagt ggtcatagcc cggtgcaata
    16681 tcggtagtta gcggccccag agtgtaaaac ggcgcttcgt ggcagtgctc taactcctcg
    16741 gtcatattgc ggcggatcat ctgcatcggc acatggcctg ggccttcaat catcacctgt
    16801 acgtcatatt cccaggcgat tttggtcagt tcgcccagcg tatgcaattc ggcaaactgc
    16861 gcttcatcgt tggcgtcctg aatagaaccg gggcgcagac cgtcgcccag cgacagcgaa
    16921 acgtcataag cggcacagat ttcacaaatt tcgcggaagt gttgatagag gaaattttcc
    16981 tgatgatggg agaggcacca tttcgccata atcgaaccgc cgcgagagac gataccggtc
    17041 aggcgtttcg cggtcatcgg cacatagcgc agcaggacgc ccgcatggat agtgaagtaa
    17101 tccacacctt gctcggcctg ttccagcagc gtgtcgcgga acgcttccca ggtaagatct
    17161 tcggcgatcc cgttaacctt ctccagcgcc tggtagatcg gcactgtacc gatcggcacc
    17221 ggactgttac gcaaaatcca ctcgcgggtt tcgtgaatat agcgaccggt ggagagatcc
    17281 atcaccgtat ccgctcccca gcgcgtggac cataccagct tttccacttc ttcttcgatg
    17341 gaagaggtga ccgccgagtt gccgatattg gcgttaactt ttaccaggaa gttacggcca
    17401 ataatcatcg gctccgattc cggatgatta atgttggccg ggataatcgc acgtccggca
    17461 gcaacttcat cacggacaaa ttccgcagtg atattttccg gcagatgtgc gccaaagctc
    17521 attcccggat gctggtggcg taaaacctcg ctacggatgc gctcgcggcc catattctcg
    17581 cggatggcga tgaattccat ttccggcgtg atgatgccct ggcgggcgta gtgcagttgg
    17641 gtgacacggc gtcctgtttt ggcgcgtttt ggcgttaata agccgctaaa acgtagctcg
    17701 tcgagaccat catctgccag ccgcgttctg gtgtaatcgg aactgcgcac ggtaagttct
    17761 tcggtatcgg cgcgcgcatc gatccacggc tggcgtagtt ttgccagccc ttgctgcacg
    17821 ttaatggcga tctgcggatc accatacggg ccggaggtgt cgtagaccgg aatcgcttcg
    17881 ttttcttcgt actgcggctg ttctttgcta ccgccaatta gcgtcgggct aagctggatc
    17941 tcacgcatcg gcacgcgcac gccggggtgt gtgccagtga tataaatgcg ttttgagttg
    18001 ggaaaggcgg tgccttccag ggtgtcgata aaatgttggg cccgggcgcg ttgttcgcgg
    18061 cgggtcagtt ttgttgcaga catagctcat tccaaaaagt taaggacgtg gcttgtcaga
    18121 cgacggatga agcaagagac gatcgccgca aaggcgatgc gatagcagat taactcttgt
    18181 tcccttcgca ggtattagcc tgatcaggtt ccgcggatcc cgaataaacg gtctcagcca
    18241 gttaaggcac tccgacaaga aattagcccc cacaaacggg gcgttgaatg taaattacgc
    18301 gttaacagcg cagaactcaa gcaggatgtt tgacgcgggc ggcgtcaagc accagcagaa
    18361 tcagcttatc ttccagcaca aagcgcgctt ccagcgcctc gccgatgtca gataaaacct
    18421 gttgaaactc aaggtaatta tcatgatcga tggcggtttc caggctggaa tcgtagtaat
    18481 ccataatctg ttgggtattg gcttcgagtt gcggccaaat cttcgcggcg cgcgcgagtt
    18541 gcccgttgcc ttccagctta tgaagaatgc gctcataaat actgaaatgt ccggcagaca
    18601 agtaatcgac caggctctga caaaaatcat caagggcttt ttcgtttagc ctcatgtacg
    18661 attctttgcc aggcttaatg ccaaccagat tgtagtaagc cacgagcaga tgcttacgta
    18721 catgtagcca gcgatcaacc agtttgttac ttcctctgac gcgctccgtc aggttatcga
    18781 gctggttaag catgattgac tccgcaagtt tgtattcaaa aactgctcag tgagaaatgt
    18841 aaaaaccatg ttaaacatgc cagtgatgca aaggtagtgc aagagctatg gatcgtataa
    18901 ttgaaaaatt agatcacggc tggtgggtcg tcagccatga acaaaaatta tggttgccga
    18961 agggagaatt gccatatggc gaagcggcaa atttcgatct tgtgggtcag cgcgcactac
    19021 aaatcggcga atggcaggga gaacctgttt ggttaataca acagcagcgg cgtcacgata
    19081 tggggtcggt acgtcaggtc attgatctcg atgttgggct gtttcaactg gccggacgtg
    19141 gcgtgcaact ggcggagttt taccgatcgc ataaatactg tggttactgc gggcatgaaa
    19201 tgtatccgag caaaaccgaa tgggcgatgc tgtgcagcca ttgccgtgag cgttactacc
    19261 cgcaaatcgc cccctgcatt attgttgcca tccgtcgcga tgattcgatc ctcctcgccc
    19321 agcatacccg ccatcgtaac ggtgtccata cagtacttgc cggattcgtc gaagtgggcg
    19381 aaaccctcga gcaggcagtc gcgcgggaag tgatggaaga gagcggaatt aaagttaaaa
    19441 acttgcgtta cgtgacttct cagccgtggc cgtttcctca gtctttaatg accgcgttta
    19501 tggcggaata tgacagcggc gacatcgtga tcgacccgaa agaattgctc gaggcgaact
    19561 ggtatcgcta tgacgatttg ccgttactcc cgccgcccgg caccgtagcg cgccgtctga
    19621 tagaagatac tgtggcgatg tgtcgggcag agtatgagtg atgatacact gaccgcctga
    19681 cgcactaagg aacagccaaa atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc
    19741 tgcgccagcc cgttgatgtc actccagtat ggatgatgcg ccaggcgggt cgctatctac
    19801 cggaatataa agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc aaaaacgccg
    19861 agctggcgtg cgaagtgact ttgcaaccgc tgcgtcgcta cccgctggat gcggcgatcc
    19921 tcttttccga tatcctcacc gtgccggacg cgatggggtt agggctctat tttgaagccg
    19981 gagaaggtcc gcgttttacc tcgccagtca cctgcaaagc cgacgtcgat aaactgccaa
    20041 ttccggaccc ggaagatgag ctgggttacg tgatgaacgc ggtgcgtacc attcgtcgcg
    20101 aactgaaagg cgaagtgccg ctgattggtt tttccggcag cccgtggacg ctggcaacct
    20161 acatggtgga aggcggcagc agcaaagcct tcaccgtgat caaaaaaatg atgtatgccg
    20221 atccgcaggc gctgcacgct ctgctcgata aactggcgaa aagcgtcact ttgtatctga
    20281 atgcgcagat taaagccggt gctcaggcag tgatgatttt cgacacctgg ggcggcgtgc
    20341 ttaccgggcg cgattatcaa cagttctcgc tctattacat gcataaaatt gttgatggtt
    20401 tactgcgtga aaacgacggt cgccgcgtgc cagtcacgct gtttaccaaa ggcggcggac
    20461 agtggctgga agccatggct gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg
    20521 acatcgccga tgcgcgccgc cgtgtgggca ataaagtcgc gttgcagggt aatatggatc
    20581 cgtcgatgct gtacgcgccg cctgcccgca ttgaagaaga agtagcgact atacttgcag
    20641 gtttcggtca cggcgaaggt catgtcttta accttggtca cggcattcat caggatgtgc
    20701 cgccagaaca tgctggcgtg ttcgtggagg cagtgcatcg actgtctgaa cagtatcacc
    20761 gctaagagcc tgatccatca ggctatttta tttgccattt tgaggccggg cagtgctcaa
    20821 aatcctcacg tactacgtgt acgctccggt ttttccgcgc tgtccggcct caaactgcct
    20881 tcaacaatta cgcctgttga accaggctct aaggagcgat tatggatctc gcgtcattac
    20941 gcgctcaaca aatcgaactg gcttcttctg tgatctgcga ggatcgactc gataaagatc
    21001 caccggatct gatcgccgga gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag
    21061 cggcgatggt gctgctgaaa tatccctcgc ttgagctggt ggagtataaa gttgcccgca
    21121 tcgccaccac catgccttac attccaggtt ttctttcctt ccgcgaatat cctgcgctgc
    21181 tggcagcgtg ggagatgctg tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga
    21241 tctcgcatcc tcgccgtctt ggcgtcgcca gccattttgg cttattggtg gatgtgccga
    21301 ccattggcgt ggcgaaaaaa cggctctgcg gtaaattcga accgctctcc agcgaaccgg
    21361 gcgcgctggc cccactgatg gataaaggcg agcagctggc ctgggtctgg cgcagcaaag
    21421 cgcgctgtaa cccgttgttt atcgctaccg gccatcgggt cagcgtggac agcgcgctgg
    21481 cgtgggtaca acgctgcatg aaaggctatc gtctgccgga gccaacgcgc tgggcggacg
    21541 cggtggcctc ggaacgtccg gcgttcgtgc gctatacagc aaatcagccc taattcaggt
    21601 aaactgcgga taatttccgt atttgagaac tcaacatgtt acaaaaccca attcatctgc
    21661 gtctggagcg cctggaaagc tggcagcatg tcactttcat ggcttgctta tgcgaacgca
    21721 tgtaccccaa ttacgccatg ttctgccagc aaaccggttt tggtgatggg caaatttacc
    21781 gtcgtattct cgatctcatc tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg
    21841 acagccaact ggagaaattt gaagaagcga ttccttcagc cgacgatttc gatctgtacg
    21901 gcgtttatcc ggcaatcgat gcctgcgtgg cgttaagtga actggtccat tcgcgtttga
    21961 gtggtgaaac gctcgaacac gcggtggaag tgagtaagac ctccatcacg accgttgcga
    22021 tgctggaaat gactcaggct ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag
    22081 ctgtagagca agaatgggac attcagtggg aaatattccg acttttagct gagtgcgaag
    22141 aacgcgatat cgagctgata aaaggcctta gggcagacct gcgtgagtcg ggtgagagca
    22201 atattggtat aatttttcag caataagacc agaaaacgtg atttaacgcc tgatttgtcg
    22261 tacctggagt cttccctttc gccccccgtc tggtctacat ttggggggcg aaaaaaagtg
    22321 gctatcggtg cgtgtatgca ggagagtgct attctggcat ttccgtcgca ctcgatgctt
    22381 agcaagcgat aaacacattg taaggataac ttatgaacaa gactcaactg attgatgtaa
    22441 ttgcagagaa agcagaactg tccaaaaccc aggctaaagc tgctctggag tccactctgg
    22501 ctgcaattac tgagtctctg aaagaaggcg atgctgtaca actggttggt ttcggtacct
    22561 tcaaagtgaa ccaccgcgct gagcgtactg gccgcaaccc gcagaccggt aaagaaatca
    22621 aaattgccgc agctaacgta ccggcatttg tttctggcaa ggcactgaaa gacgcagtta
    22681 agtaagattg cgtggcagtg aacagtttta acgaaggggt ggtttcaccc cttttgtctt
    22741 tctggcgtcg atcattgatg ctggcaggtg
//
LOCUS       DADLSB010000053        22176 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.053, whole genome shotgun sequence.
ACCESSION   DADLSB010000053 DADLSB010000000
VERSION     DADLSB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.053"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            139..1320
                     /gene="nimT"
                     /locus_tag="JN331_002007"
     CDS             139..1320
                     /gene="nimT"
                     /locus_tag="JN331_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HAY7260159.1"
                     /translation="MTCSTSLSGKNRIVLIAGILIIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            1375..1722
                     /locus_tag="JN331_002008"
     CDS             1375..1722
                     /locus_tag="JN331_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY7260160.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(1744..1998)
                     /gene="yoaF"
                     /locus_tag="JN331_002009"
     CDS             complement(1744..1998)
                     /gene="yoaF"
                     /locus_tag="JN331_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY7260161.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            2181..2738
                     /gene="dgcP"
                     /locus_tag="JN331_002010"
                     /pseudo
     CDS             2181..2738
                     /gene="dgcP"
                     /locus_tag="JN331_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
     gene            2782..3747
                     /gene="yeaX"
                     /locus_tag="JN331_002011"
     CDS             2782..3747
                     /gene="yeaX"
                     /locus_tag="JN331_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY7260162.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(3801..4916)
                     /gene="rnd"
                     /locus_tag="JN331_002012"
     CDS             complement(3801..4916)
                     /gene="rnd"
                     /locus_tag="JN331_002012"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY7260163.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(4998..6683)
                     /gene="fadD"
                     /locus_tag="JN331_002013"
     CDS             complement(4998..6683)
                     /gene="fadD"
                     /locus_tag="JN331_002013"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY7260164.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEVKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(6888..7469)
                     /gene="yeaY"
                     /locus_tag="JN331_002014"
     CDS             complement(6888..7469)
                     /gene="yeaY"
                     /locus_tag="JN331_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY7260165.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTLQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(7509..8204)
                     /gene="tsaB"
                     /locus_tag="JN331_002015"
     CDS             complement(7509..8204)
                     /gene="tsaB"
                     /locus_tag="JN331_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY7260166.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(8262..10172)
                     /locus_tag="JN331_002016"
     CDS             complement(8262..10172)
                     /locus_tag="JN331_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY7260167.1"
                     /translation="MTDDFAPDGQLAKAIPGFKSREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQRALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            10304..10648
                     /locus_tag="JN331_002017"
     CDS             10304..10648
                     /locus_tag="JN331_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7260168.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11010..11369
                     /locus_tag="JN331_002018"
     CDS             11010..11369
                     /locus_tag="JN331_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY7260169.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11489..11668)
                     /locus_tag="JN331_002019"
     CDS             complement(11489..11668)
                     /locus_tag="JN331_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY7260170.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            11742..13103
                     /gene="pabB"
                     /locus_tag="JN331_002020"
     CDS             11742..13103
                     /gene="pabB"
                     /locus_tag="JN331_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY7260171.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            13107..13685
                     /locus_tag="JN331_002021"
     CDS             13107..13685
                     /locus_tag="JN331_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY7260172.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            13869..15233
                     /gene="sdaA"
                     /locus_tag="JN331_002022"
     CDS             13869..15233
                     /gene="sdaA"
                     /locus_tag="JN331_002022"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7260173.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            15364..16962
                     /locus_tag="JN331_002023"
     CDS             15364..16962
                     /locus_tag="JN331_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7260174.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(16966..18516)
                     /gene="yoaE"
                     /locus_tag="JN331_002024"
     CDS             complement(16966..18516)
                     /gene="yoaE"
                     /locus_tag="JN331_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY7260175.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            18979..19950
                     /gene="manX"
                     /locus_tag="JN331_002025"
     CDS             18979..19950
                     /gene="manX"
                     /locus_tag="JN331_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY7260176.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            20013..20813
                     /locus_tag="JN331_002026"
     CDS             20013..20813
                     /locus_tag="JN331_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7260177.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            20826..21677
                     /locus_tag="JN331_002027"
     CDS             20826..21677
                     /locus_tag="JN331_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY7260178.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            21732..>22176
                     /locus_tag="JN331_002028"
     CDS             21732..>22176
                     /locus_tag="JN331_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY7260179.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         5353 a         5756 c         5572 g         5495 t
ORIGIN      
        1 tcggtcgggt tcatagccat tgagattcaa cctgtgcatc attttgtccg aacttagcga
       61 taatttgtca ttttagcttg attcaacata acaataaaaa cggtaaggta cagcctcgtt
      121 tgtaacaatg agaagcatat gacctgttca acttcattaa gcggcaaaaa caggattgtc
      181 cttatcgctg gcattctgat aattgccaca acattacgcg tcacctttac cggcgcagcg
      241 ccgttactgg atacgattcg ttccgcttac tcgctgacga cagcgcaaac cggcttatta
      301 accaccctgc cattattggc ctttgcgcta atctcacctc tggctgcccc ggtggcgcga
      361 cgttttggta tggaacgtag cctgtttgcc gcgttacttt tgatctgtgc aggtatcgca
      421 attcgttctc tcccttcgcc ttacttatta tttggcggta cagcggtcat tggcggtggg
      481 attgcattag gcaatgtctt actgccagga ttaattaaac gcgatttccc tcattccgtc
      541 gccagactta ccggcgcata ttccctgaca atgggagctg cagcggcact gggatcggct
      601 atggtcgtgc cgctggcttt gaacggtttt ggctggcaag gcgcgttgtt catgctgatg
      661 tgttttcctc tgctggctct ttttttatgg ctgccacagt ggcgaagtca gcaacatgca
      721 aatttgagta cctcacgcgc cttacatact cggggtatct ggcgttcgcc acttgcctgg
      781 caggtaacat tgtttcttgg gatcaactca ctggtctatt acgtgattat tggctggctt
      841 ccggcgatcc tcatcagtca cggctatagc gaagcacagg cgggttcact gcatggtttg
      901 ctgcaactag ccacagcagc tcccggtttg ctgatcccac ttttcttaca tcatgtgaaa
      961 gaccagcgtg gtattgcagc gttcgttgcc ttgatgtgcg cagtgggcgc ggttgggctc
     1021 tgctttatgc cagcgcacgc gatcacctgg actctgcttt tcggttttgg ttccggcgca
     1081 acaatgatac tggggttgac gttcattggt cttcgggcaa gttctgcgca tcaggcggcg
     1141 gcactctcgg ggatggcaca atccgtcggg tatttgctgg cagcctgtgg gccgccgcta
     1201 atgggtaaaa tacacgatgc taacggtaac tggtccgtac cactgatggg tgttgcaata
     1261 ctttcactac tgatggcgat tttcggactt tgcgccggga gagacaaaga aattcgctaa
     1321 tatccggtgc tatagtgacg tgacaaatct tgcgtgaaag ggagaacaaa cacgatgaat
     1381 attcagtgca aacgcgttta tgatccggct gaacagagcg atggttatcg cgtactggtc
     1441 gaccgcctct ggccgcgcgg tatcaaaaaa accgatttag cccttgatga gtgggataaa
     1501 gaaatcacgc cgtcaacgga actgcgcaaa gcctttcacg gcgaagtcgt cgattttgca
     1561 acctttcgcg agcaatatcg cgcagaactg gcgcaacacg agcaagaagg aaagcggctg
     1621 gcggacatcg ccaaaaaaca gccgctgacc ctgctctacg cagcaaaaaa caccacgcag
     1681 aaccatgcgc tggtgctggc cgactggcta cgtagcttgt gattttagta cggcatccgg
     1741 cggttatttt tcaccagccg gatggtcccg tcgccacaat gcccattcat caatcgtttc
     1801 accgcccggt aatttgcaat tgttgctgac cccttgcgct gtctgcactg gaatgagcgt
     1861 cccgcccttc tgctggcaat agaccgacgc cggatttgcc ataccaatct gcggcggttt
     1921 aggtgcttct ggctgagaag gggttgaaca accagccagg accagcaagc aaggcagaac
     1981 aaaactgata attttcattt attgatctca catatttatc caagcttaga gtatcgcggt
     2041 atcgttttgt tttgcagcac tatttttatt acattcactc aaaacatatt acgtcttgtt
     2101 tcatctttgt tgatgatgtt ttatcatgcc tgcaaagatt aaataatcag cacttacccg
     2161 ccgtatcctg gagttgttcc gtgtcagatc agattatcgc ccgcgtctcg caatcccttg
     2221 ccaaagaaca gtcactggaa agtctggtcc gacagcttct ggagatgctg gaaatggtca
     2281 ctgatatgga atcaacctac ctgaccaaag tggatgtcga agcgcgcctg cagcatatta
     2341 tgtttgcccg taacagccag aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt
     2401 actcgttatg caaacgcgcc attgatgaaa actgcttttt cagcgatgaa gtccctgacc
     2461 gttggggcga ctgtattgcg gcacgcaatc ttggcatcac cacatttctg agcacgccaa
     2521 ttcacttacc ggatggatca ttctatggca cgctttgcgc cgccagcagt gagaagcgcc
     2581 agtggagtga acgcgcggaa caggttttac agttattcgc cggactgatt gcacaatata
     2641 ttcaaaaaga ggcactggtt gaacagctgc acgaagccaa tgctgcgctg attgcgcaat
     2701 cgtataccga ctcgttaacc gggctaccga atcggcggcg gtattttccg ccgctgggct
     2761 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagccag
     2821 gttgaacccc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
     2881 ctgcctgcgt tcaccggagg aagtcacatc ctagtgcaga tgagcgatgg tgataaccag
     2941 tacagcaatg cgtattcact actgagttca ccgcataaca cctctagtta tcagattgcc
     3001 gttcgactgg aagaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
     3061 ggcgatcggt taacgatctc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
     3121 aagcatctgt ttatcgcggg cggtattggt atcacccctt tcctgtcgca catggcagag
     3181 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
     3241 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattca
     3301 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
     3361 gtttatacct gtggccccga ggcgctaatt gaagcggtaa gaagtgaagc tgcgcgtctg
     3421 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
     3481 gcatttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
     3541 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
     3601 gtatgcggaa cctgcgaaac ggcaatactg gaaggtgaag ctgaccatcg ggatcagtat
     3661 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
     3721 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
     3781 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
     3841 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
     3901 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
     3961 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
     4021 taaccaggca tgtccatcag gttaagcatc ggctgcggta aggcatcttc cggcaatgtc
     4081 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
     4141 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
     4201 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct ctcgcgcctt gcgcagtcgc
     4261 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaactgcca ggcattggtg
     4321 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
     4381 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
     4441 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
     4501 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
     4561 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
     4621 gtgtcaatca agggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
     4681 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
     4741 cagtcggtga tcccgagagg atcaattagc gccagatgct cgccatcgaa aagttgaatc
     4801 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatccag ggctatcgcc
     4861 ggaaaggcac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
     4921 taattcaaat tgttttctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
     4981 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
     5041 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
     5101 ttcggtactt tgtatcccgt gagctgacgg cggcaaaaag tcaccagtga ctcttcggta
     5161 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
     5221 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
     5281 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
     5341 aggaatcctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
     5401 atctcatctg tagcatccgg acgctgccag taacccagca tcacctgcgg tcctttgaca
     5461 caaagctcac ccggttgacc cggtggtact tcgttgtcgt cgtcatccac cagtttgact
     5521 tccgtcgatg gcaccggcaa accgatgcta ccgctatgat aatcaatatc atacgggtta
     5581 acgctcacca gcggcgcgca ctcggtcagg ccataacctt ccagcagata ctgtccggtc
     5641 agtttcaccc agcgttctgc caccacttgc tgaacaggca tcccaccgcc tgcggaaaga
     5701 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
     5761 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
     5821 atatcacgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
     5881 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
     5941 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
     6001 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
     6061 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
     6121 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
     6181 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
     6241 attacgtgct gaacggcggt tttatcaacc actttttcca gagtgtgggc aaagttagac
     6301 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
     6361 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa tagcgccacc
     6421 ggatattgca ataagttagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
     6481 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatc
     6541 acctccccca tattcacaaa cgcaggctga tcggcgtagc gcgcgaccga ctgctcaaac
     6601 atatctacca gagattgata acggtcaggg ttgatctccg tcggaacgtc cgcgggataa
     6661 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
     6721 accccaaatg atatacatgc tgttaacata atattaactc atcataccag cttgataatt
     6781 acccaacgaa aaggttgcga agcgcgtcac tatttatttt tatctttacc gtaagaatgc
     6841 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caagcaatta ttcggttaca
     6901 actgtttgta ctctcgcggg gccgggatta taccagcccc atccgccgta gccatagggc
     6961 cagccacgac cgccataaaa ccatggatca atcggctgag gcggcataat cacctgcagg
     7021 gttaaatgcc aacgtttgta acccgttact tgcatcacca taaatttata gggcgtattg
     7081 ccgattttgc cgtcaaccgc accggtgatt ggcccgacta ccgtaaccag ttgtccacgg
     7141 aaatccaccg ggtccagaaa accgttcaca tcggcataaa tgcgaccgcg agaaggttct
     7201 cccagcgtcg gcctggctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
     7261 ccttgctggt tttgtaccgc aaccactttg ccaccaaagc gtgcctcctg accaacgtac
     7321 agctgcggcg cactcatcac ccgaactaaa tcttgttgcg gcgtgggact gctgccttta
     7381 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
     7441 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
     7501 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg caaataaacc
     7561 ggctcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca agcaatcggc
     7621 agcatatctt ctgcagcggg gagtaacact tcgccatcgc gcaaaaccag cccgctctct
     7681 ttaccgagat ccggccaggc ttgccagccc gtccctaccg tcacccattc gccggaaagc
     7741 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgtgc
     7801 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
     7861 atggctgaca gcacgcgtgt tgcgccgttt ttgcgccacg ccccttgcgc catcgtcatt
     7921 agcgtggaga cgccaatcat cggtaattcc gcgccaagcg ccagcccttg cgcgatgcca
     7981 ataccaatgc gcacgccggt aaagctaccg gggccgcgcc cgtaagccag agcgttaata
     8041 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
     8101 gtatgttcac gagggcaaag ctcaaaatga gcgttgacag taccgtcgtt ccacagggcg
     8161 acagagcagg cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
     8221 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
     8281 gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg
     8341 agaaacgtcg cgccgtaagg acgcatcacc agccgatagt cacaaatcac cagcacgcca
     8401 cgatcgtcgg cgtcgcgaat cagtcgacct accccctgtt tgagagtaat gacggcatct
     8461 ggtagttgca cttcatcgaa cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct
     8521 tttaacagtg gatcatccgg cgaggtaaac ggcaatttgt cgataattac caatgacaat
     8581 gtatcgccac gcacgtccac cccttcccag aaactactgg ttgccacaag aagcgcatta
     8641 ccggcactga caaactgctg caacagttgg cctttactgg tttccccctg caacaaaacg
     8701 ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc gtgcgaggta
     8761 caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat tgccgctaac
     8821 tggcgagcag aacctggctg gttggtttgc ggcagattgc gcggcacaca gagtaacgcc
     8881 tggcggctgt aatcaaacgg gcttggcaat agcaacgact ccgcctgttc tatcccaagc
     8941 cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt gaagatccag
     9001 ctaccgggct tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgaga
     9061 gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg ctgattgatc
     9121 tcttttagcc gcttcagccg tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc
     9181 aaggcggaac gccccagtga cagtttcgcc acgtcataac aaagttccaa ggtgtcatcg
     9241 agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag gttaccgcga
     9301 taacctggct caccgagttg cagacgaaaa tcctgcgcac tctgggcaag acgatcagcg
     9361 cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt
     9421 gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct ggcaatgtcc
     9481 ggtagctggt gggcttcgtc gaagatcatg acgtccgctt cagggatcaa ctcgccaaat
     9541 ccactctctt taaccaccat atccgccaga aagagatgat ggtttaccac caccacatcg
     9601 gcgtccatcg cttttttacg tgctttgacc acaaagcaat ctttatacat cgggcagtcg
     9661 ctgccaagac agttgtcgtt ggtgctggtg accagcggcc acgcctgtga atcttccgcc
     9721 acgctgacgc aggtgctgat atcaccatcg actgtttgat tagaccagga gcgcagcagg
     9781 atcacatcgc ttaagatttg taccggcaga tcgccccccg ccagcgcccg ctgttcgaga
     9841 cgttcgaggc agaggtagtt tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc
     9901 aatgcctttg agactgttgg caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag
     9961 ccggtcgaga taatgacttt ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt
    10021 ttgcccgtac cggttcctgc ttccaccacc agcggctggc ctttttctat cgcctgggtg
    10081 acggctaccg ccatctgtcg ctgtggttct cgcgacttaa agcctggtat cgctttcgcc
    10141 agctgaccgt ctggtgcaaa atcgtccgtc acactacccc ctgttgattt gaacagggat
    10201 tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcaacctc acattgtggc
    10261 agtctttgca gcacgaaaat ggaagtataa tgaggacaaa atgatgacta tcgttcgtat
    10321 cgatgctgaa gcccgctggt ctgatgtagt aatccacaac aacacgctct actacactgg
    10381 tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacacgc tggcacagat
    10441 tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg ccaccatttt
    10501 cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc
    10561 gggtcatgcg ccggtgcgct gcacggtaca agcgggtttg atgaacccga agtataaagt
    10621 tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt cagacgataa
    10681 tttatcgata atactggtcg gttttacata aatcgaccag agaataagat attactgttg
    10741 atgcttctgc taattcatta tttatattta taatttcaat tttatctata aaactaacta
    10801 tataaataaa cataaccatg ccgttgtgtt cgccctttga ttagcaactc tggttttctt
    10861 tcttagctga acaatctggt tatagcgtgc gggctgaccg ttaattttct ccatcttact
    10921 ggataacaca ccatgagctg ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg
    10981 ccatatcgtt ataatccctt tgatattaga tgcaaattaa ggtcatatat agccttatcg
    11041 ataacatggt taattttaag gataagaata tgcctgctgt aatagataaa gccctggatt
    11101 tcattggtgc catggatgta tcagcgccaa caccaagttc gatgaatgaa agcacggcga
    11161 agggaatctt taaatattta aaagaactgg gagtacccgc gagtgccgct gatattacgg
    11221 cgcgagctga ccaggaaggc tggaaccctg ggttcacgga aaaaatggtt ggatgggcaa
    11281 aaaaaatgga gacaggtgaa cgctctgtga ttaaaaatcc tgaatacttt tcaacatata
    11341 tgcaggaaga actaaaagca ctggtctgag ttaaatttat atcagcataa atgggtcagg
    11401 acgcttttaa tcacatataa aaagcgtcct ttttctcaca atcaaatgat catcggcttc
    11461 tataacgaaa tcgatacgga ttagtgtgtt actcgtcttc atcctcaaaa cgcgccacga
    11521 ttcgctcgcc ggagtggttg gcacgcagct cttctgccac cagcgcgatt gcctggccgc
    11581 tgctcatccc ttgtgccatc agttcctgga tccgctcgac agctttttgc tgctgttcat
    11641 gggtgagtga aggtaaacct gcaaacattg ttaactcctg ctaaattgtt ggcgctaatt
    11701 atttcatgct acccggcaca tagccagtag agtcaggact gatgaagacg ttatctcccg
    11761 ctgtgattac tttaccctgg cgtcaggacg ccgctgaatt ttatttctcc cgcttaagcc
    11821 acctgccgtg ggcgatgctt ttacactccg gctatgccga tcatccgtat agccgctttg
    11881 atattgtggt cgccgatccg atttgcactt taaccacttt aggtaaagaa accgttgtta
    11941 gtgaaagcga aaaacgcaca acgaccactg atgacccgct acaggtgctc cagcaggtgc
    12001 tggatcgcgc agacattcgc ccaacgcata acgaagattt gccatttcag ggcggcgcac
    12061 tggggttgtt tggctacgat ctgggccgcc gttttgagtc actgccagaa attgcggaac
    12121 aagatatcgt tctgccggat atggcagtgg gtatctacga ttgggcgctc attgtcgacc
    12181 accagcgtca tacagtttct ttgctgagtc ataatgatgt caatgcccgt cgggcctggc
    12241 tggaaagcca gcaattctcg ccgcaggaag atttcacgct cacttccgac tggcaatcca
    12301 atatgacccg cgagcagtac ggcgaaaaat ttcgccaggt acaggaatat ctgcacagcg
    12361 gtgattgcta tcaggtgaat ctcgcccagc gttttcatgc gacctattct ggcgatgaat
    12421 ggcaggcatt ccttcagctt aatcaggcca accgcgcgcc atttagcgct tttttacgtc
    12481 ttgaacaggg tgcaatttta agcctttcgc cagagcggtt tattctttgt gataatagtg
    12541 aaatccagac ccgcccgatt aaaggcacgc taccacgcct gcccgatcct caggaagata
    12601 gcaaacaagc agaaaaactg gcgaactcag cgaaagatcg tgccgaaaat ctgatgattg
    12661 tcgatttaat gcgtaatgat atcggtcgtg ttgccgtagc cggttcggta aaagtaccag
    12721 agctcttcgt ggtggaaccc ttccctgccg tgcatcatct ggtcagcact ataacggcgc
    12781 gactaccaga acagttacac gccagcgatc tgctgcgcgc agcttttcct ggtggctcaa
    12841 taaccggggc tccgaaagta cgggctatgg aaattatcga cgaactggaa ccgcagcgac
    12901 gtaatgcctg gtgcggcagc attggctatt tgagcttttg cggcaacatg gataccagca
    12961 ttactatccg cacgctgact gccattaacg gacaaatata ctgctctgcg ggcggtggaa
    13021 ttgtcgccga tagccaggaa gaagcggaat atcaggaaac ttttgataaa gttaataaga
    13081 tattacgcca actggagaag taagacgtgg aataccgtag cctgacgctt gatgattttt
    13141 tatctcgctt tcaacttttg cgcccgcaaa ttaaccggga aaccctaaat catcgtcagg
    13201 ctgccgtgtt aatccccatc gtccgtcgac cgcaaccggg gttgttgctg actcagcgtt
    13261 caattcatct gcgtaaacac gctggacaag tggcattccc tggaggtgca gtcgatgaca
    13321 cggacgcatc agttatcgcc gccgcgctgc gcgaagctga agaagaggtc gctataccgc
    13381 cttccgccgt tgaagttatc ggcgtgctgc cgcccgtcga tagcgtaact ggctatcagg
    13441 taaccccagt ggtcggcatt atcccgcccg atctgccgta tcgcgccagt gaagatgaag
    13501 tctcggcggt gtttgaaatg ccgctcgccc aggcattaca tctgggtcgt tatcaccctt
    13561 tagatatcta ccgccgtggc gattcacatc gggtatggct gtcctggtac gaacagtatt
    13621 ttgtatgggg aatgaccgca ggcataattc gtgagctggc gctgcaaatt ggtgtgaacc
    13681 cctgactata cttatcttta catctacaaa acactacttg agacaatcat cgcaatatta
    13741 gttaaatcgc ggtttttgat tagtttaatt catgtgaata gttaagccag tcgccgcgtt
    13801 ccctcttaca ctatgcgctg ttattagttc gttactggaa gtccagtcac cttgtcagga
    13861 gtattatcgt gattagtcta ttcgacatgt ttaaggtggg gattggtccc tcatcttccc
    13921 ataccgtagg gcctatgaag gcgggtaaac agttcgtcga tgatctggtc gaaaaaggct
    13981 tactggatag cgttactcgc gttgccgtgg acgtttatgg ttcactgtcg ctgacgggta
    14041 aaggccacca caccgatatc gccattatta tgggtcttgc aggtaacgaa cctgccaccg
    14101 tggatatcga cagtattccc ggttttattc gcgacgtaaa agagcgcgaa cgtctgctgc
    14161 tggcacaggg acggcatgaa gtggatttcc cgcgcgacaa cgggatgcgt tttcataacg
    14221 gcaacctgcc gctgcatgaa aacggtatgc aaatccacgc ctataacggc gatgaagtcg
    14281 tctacagcaa aacttattat tccatcggcg gcggttttat cgtcgatgaa gaacactttg
    14341 gtcaggatgc tgccaacgaa gtgagcgtgc cgtatccgtt caaatctgcc accgaactgc
    14401 tcgcgtactg taatgaaacc ggctattcgc tgtctggtct cgctatgcag aacgaactgg
    14461 cgctgcacag caagaaagag atcgacgagt atttcgcgca tgtctggcaa accatgcagg
    14521 catgtatcga tcgcgggatg aacaccgaag gtgtactgcc aggcccgctg cgcgtgccac
    14581 gtcgtgcgtc tgccctgcgc cggatgttag tttccagcga taaactgtct aacgatccga
    14641 tgaatgtcat tgactgggta aacatgtttg cgctggcagt taacgaagaa aacgccgccg
    14701 gtggtcgtgt ggtaactgcg ccaaccaacg gtgcctgcgg tatcgttccg gcagtgctgg
    14761 cttactatga ccactttatt gaatcggtca gcccagacat ctatacccgt tactttatgg
    14821 cagcgggcgc gattggtgca ttgtataaaa tgaacgcctc tatttccggt gcggaagttg
    14881 gttgccaggg cgaagtgggt gttgcctgtt caatggctgc tgcgggtctt gcagaactgc
    14941 tgggcggtag cccggaacag gtttgcgtgg cggcggaaat tggcatggaa cacaaccttg
    15001 gtttaacctg cgacccggtt gcagggcagg ttcaggtgcc gtgcattgag cgtaatgcca
    15061 ttgcctctgt gaaggcgatt aacgccgcac ggatggctct gcgccgcacc agtgcaccgc
    15121 gcgtctcgct ggataaggtc atcgaaacga tgtacgaaac cggtaaggac atgaacgcca
    15181 aataccgcga aacctcacgc ggtggtctgg caatcaaagt ccagtgtgac taatacttct
    15241 tactcgccca tctgcaacgg atgggcgaat ttatacccgc tttctcgtct gctgtaatat
    15301 tccccactac acttccactg ttgcgtcagg cgtttgtcgc catacgctta cagggtggcc
    15361 cgcatgcaaa aagcacaacg gatcattaaa acctatcgcc gtaatcgaat gattgtttgt
    15421 acgatttgcg cactcgttac gctcgcttcg accctgagcg tgcgatttat ttcacagcgt
    15481 aacttaaatc aacaacgggt agtacaattc gccaatcacg ctgtagagga attagataaa
    15541 gtactgcttc ccctacaggc aggtagcgaa gtcttgcttc cgctgattgg tctgccctgc
    15601 tctgtcgccc atttgccatt acgtaaacag gcggcaaaac tccaaactgt gcgatccatt
    15661 ggcctggtgc aagacggcac actttattgc tccagcattt ttggttatcg caatgtgccc
    15721 gtcgtggaca ttctggctga acttcctgca ccgcaaccac ttttacgcct gacgatcgac
    15781 cgtgccctga ttaaaggcag tccggttttg attcaatgga cgcctgcagc gggcagtagc
    15841 aatgctgggg tcatggagat gattaacatc gacttactga cggcaatgct ccttgagcca
    15901 caactgccgc aaatcagtag cgccagcctg acggtggaca aacggcattt gctctatggt
    15961 aatgggctgg tagattccct tccgcaacct gaagacaatg aaaactatca ggtttcttcg
    16021 caaagctttc cttttaccat taacgtaaat ggtccggggg ctacggcgct ggcatggcac
    16081 tatcttccaa cacaattacc gctggcggtg ctgctaagtt tactggtggg ctatatcgcc
    16141 tggctggcga ccgcttaccg gatgagcttt tcccgcgaaa ttaatctggg cctggcgcaa
    16201 catgagttcg aattgttctg tcagcctttg cttaatgcgc gcagccagca atgtattggt
    16261 gtagagattt tgctgcgctg gaataatccg cgtcagggct ggatttcacc ggatgtgttt
    16321 attcctatcg cggaagaaca tcatttaatt gtgccactga cccgctatgt gatggcagaa
    16381 accattcgtc agcgccatgt tttcccgatg agtagtcagt ttcatgttgg cattaacgtc
    16441 gcacccagcc attttcgccg tggtgtgctg ataaaagatc tcaatcagta ctggtttagc
    16501 gctcacccga ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt actggatgtt
    16561 gattatcgga ttgcccgcga gctgcatcgt aaaaacgtca aactggcgat tgatgacttc
    16621 ggcaccggca acagctcgtt ttcctggctt gaaacattac gtcctgacgt gctgaaaatt
    16681 gataagtcat ttaccgcagc tataggttct gacgcggtta actcgacggt gaccgatatc
    16741 atcatcgcgc tggggcaaag actgaatatt gaactggtgg cggagggcgt ggaaacgcaa
    16801 gaacaggcga agtatttgcg ccgtcatggc gtgcatattt tgcaagggta tttgtacgca
    16861 cagccgatgc cgctacgtga ttttcccaaa tggctggcgg gcagccaacc gccgcccgcc
    16921 cggcataatg gacatatcac gcccattatg tcgttacgtt aacgcttact catcttcatc
    16981 gtgcgccggt tgctctttaa caatgcgaac cagatcaaca cgataatcat tggcttcaag
    17041 gatggtgata tgcagtggcc ctacatcaat cacatcgccc acacggggaa tgtgaccatt
    17101 tgccgagatc acgaggcccg cgaccgtcgc gatatcatcg tcatcggcaa ggtgctcaac
    17161 atcaagcgcc tgctgcaagg catgcaaatc tgtaccgcct tttaccagcc agccgttacc
    17221 atcggtaatg atttccggcg tttcgtcagc gtccgggaat tcacccgcaa tggcttccag
    17281 cacatccagc ggcgtgacca agccttgtac cacaccaaac tcgttggtca cgataacaaa
    17341 gctcccgcga gcgcgacgca gcacgcccag caggttgatc ggatcgaggg tttccgggac
    17401 gataatcgcc ggagacgccg aagcaatcgc cgccacatca acgccctctt ccagcgccac
    17461 cagcagttct ttagcacgta cgataccgat gatttcatcc agttcaccgc gacataccgg
    17521 gaacagactg tgcggtgaag agagcagttg ctcgcggatt tcatcgaccc cgagattagc
    17581 gtcaacccag cttatttcac cgcgcggcgt catgatcccg cgcagagaac gcgacgccag
    17641 cgtcagtacg ccgttaatca tgtaccgttc ttcttcggca aatgcacctt ccgggatcgg
    17701 catcgggtta tcggcatcgt gctgaacatt ggcctgacgt ttcccgccca tcaaacgcag
    17761 gatggcatcg gcagtacgcg ctcgcagcgg caaagtcgac tggtgacgaa taaagttgcg
    17821 acgcgcaatc tggttaaaca cttcgataat gatcgagaag ccaatcgcgg catacaggta
    17881 acctttcgga atgtggaaac cgaaaccttc tgccaccaga ctcagaccaa tcattaacag
    17941 gaagctcaga cagagcacca ccaccgtggg gtgctggtta acgaatcgcg tcagcggttt
    18001 cgatgccagc aacataaccg ccatcgcaat cactaccgcc gccatcatca ccggcagatg
    18061 gttaaccatc cctactgcag taattaccgc atccaacgag aagacggcgt caaggatgac
    18121 aatctgtgtg acgaccaccc agaaactggc gtagccttta ccgtggccgg aatcatgatc
    18181 gcggttttcc agccgttcat gcagttcggt tgttgctttg aacagcaaga atatcccccc
    18241 gaacaacata atcagatcgc gtccggagaa ggagaaatcc atgacggtaa atagcggttt
    18301 ggtcagcgtg accatccatg aaatcagcga cagcagcccc agacgcataa tcagcgccag
    18361 tgataacccc agcaaacgcg ctttatcgcg ttgttttggc ggcagtttgt cagcaagaat
    18421 ggcgatgaag accaggttat cgatacccag cacaatttcg agaacaacaa gcgtgagtag
    18481 ccccgcccaa attgaggggt ccattaagaa ttccatgaca agctcctgct taaggaatag
    18541 ctattcgacg ccagaaataa tgcaggcgta acgacaaaat gcaaacgaaa ggtgcggcat
    18601 agagtgccag aaaggcaggc gttaaaaggc ctgatgctga aatgacgtcg gtgacgatcc
    18661 atactgcggg ctactgccct atactccatg gttgttaaac gggagttaaa catatcagag
    18721 acgcctctga tttggcaaag atttaccttc ctttgcaaac gaatgtgaca aggatatttt
    18781 acctttcgaa atttctgata atcgaaagtt aaattacgga tcttcatcac ataaaataat
    18841 tttttcgata tctaaaataa atcgcgaaac gcaggggttt ttggttgtag cccttatctg
    18901 aatcgattcg attgtggacg acgattcaaa aatacatctg gcacgttgag gtgttaacga
    18961 taataaagga ggtagcaagt gaccattgct attgttatag gcacacatgg ttgggctgca
    19021 gagcagttgc ttaaaacggc agaaatgctg ttaggcgagc aggaaaacgt cggctggatc
    19081 gatttcgttc caggtgaaaa tgccgaaacg ctgattgaaa agtacaacgc tcagttggca
    19141 aaactcgaca ccactaaagg cgtgctgttt ctcgttgata catggggagg cagcccgttc
    19201 aatgctgcca gccgcattgt cgtcgacaaa gagcattatg aagtcattgc aggcgttaac
    19261 attccaatgc tcgtggaaac gttaatggcc cgtgatgatg acccgagctt tgatgaactg
    19321 gttgcgctgg cagtagaaac aggccgtgaa ggcgtgaaag cactgaaagc caaaccggtt
    19381 gaaaaagccg cgccagcacc cgctgccgca gcaccaaaag cggctccaac tccggcaaaa
    19441 ccaatggggc caaacgacta catggttatt ggccttgcgc gtatcgacga ccgtctgatt
    19501 cacggtcagg tcgccacccg ctggaccaaa gaaaccaatg tctcccgtat tattgttgtt
    19561 agtgatgaag tggctgcgga taccgttcgt aagacactgc tcacccaggt tgcacctccg
    19621 ggcgtaacag cacacgtagt tgatgttgcc aaaatgattc gcgtctacaa caacccgaaa
    19681 tatgctggcg aacgcgtaat gctgttattt accaacccaa cagatgtaga gcgtctcgtt
    19741 gaaggcggcg tgaaaatcac ctctgttaac gtcggtggta tggcattccg tcagggtaaa
    19801 acccaggtga ataacgcggt ttcggttgat gaaaaagata tcgaggcgtt caaaaaactg
    19861 aatgcacgcg gtattgagct ggaagtccgt aaggtttcca ccgatccgaa actgaaaatg
    19921 atggatctga tcagcaaaat cgataagtaa cgtattgtgt tgattatcat tcagttttca
    19981 cacttaagtc ttacgtaaac aggagaagta caatggagat taccactctt caaattgtgc
    20041 tggtatttat cgtagcctgt atcgcaggta tgggatcaat cctcgatgaa tttcagtttc
    20101 accgtccgct aatcgcgtgt accctggtgg gtatcgttct tggggatatg aaaaccggta
    20161 ttattatcgg tggtacgctg gaaatgatcg cgctgggctg gatgaacatc ggtgctgcag
    20221 ttgcgcctga cgccgctctg gcttctatca tttctaccat tctggttatc gcaggtcatc
    20281 agagcattgg tgcaggtatc gcactggcaa tccctctggc cgctgcgggc caggtactga
    20341 ccatcatcgt tcgtactatt accgttgctt tccagcacgc tgcggataag gctgctgata
    20401 acggcaacct gacagcaatt tcctggatcc acgtttcttc tctgttcctg caagcaatgc
    20461 gtgtagctat tccggccgtc atcgttgcgc tgtctgttgg taccagcgaa gtacagaaca
    20521 tgctgaatgc gattccggaa gtggtgacca atggtctgaa tatcgccggg ggcatgatcg
    20581 tggtggttgg ttatgcgatg gttatcaaca tgatgcgtgc tggctacctg atgccgttct
    20641 tctacctcgg cttcgtaacc gcagcattca ccaactttaa cctggttgct ctgggtgtga
    20701 ttggtactgt tatggcagtg ctctacatcc aacttagccc gaaatacaac cgcgtagccg
    20761 gtgcgcctgc tcaggcagct ggtaacaacg atctcgataa cgaactggac taacaggtga
    20821 gcgaaatggt tgatacaact caaactacca ccgagaaaaa actcactcaa agtgatattc
    20881 gtggcgtatt cctgcgttct aacctcttcc agggttcatg gaacttcgaa cgtatgcagg
    20941 cactgggttt ctgcttctct atggtaccgg caattcgtcg cctctaccct gagaacaacg
    21001 aagctcgtaa acaagctatt cgccgtcacc tggagttctt taacacccag ccgttcgtgg
    21061 ctgcaccgat tctcggcgta accctggcgc tggaagaaca gcgtgctaat ggcgcagaga
    21121 tcgatgacgg tgctatcaac ggtatcaaag tcggtttgat ggggccgctg gctggtgtag
    21181 gcgacccgat cttctgggga accgtacgtc cggtatttgc agcactgggt gccggtatcg
    21241 cgatgagcgg cagcctgtta ggtccgctgc tgttcttcat cctgtttaac ctggtgcgtc
    21301 tggcaacccg ttactacggc gtagcgtatg gttactccaa aggtatcgat atcgttaaag
    21361 atatgggtgg tggcttcctg caaaaactga cggaaggggc gtctatcctc ggcctgtttg
    21421 tcatgggggc attggttaac aagtggacac atgtcaacat cccgctggtg gtttcgacca
    21481 tcaccgggca ggatggacag acccgtgtca ccaccgtgca aactattctt gaccagttaa
    21541 tgccaggcct tgtaccactg ctgctgacat ttgcttgtat gtggctgctg cgcaaaaaag
    21601 ttaacccgct gtggatcatc gttggcttct tcgtcatcgg tatcgctggt tacgcttgcg
    21661 gcctgctggg actgtaagac tgttgtacac taccggggcc ttttggcccc gtttttttat
    21721 ctggaggatt aatgacaatc acggacctgg tactgattct tttcatcgcc gcactcctgg
    21781 ccttcgcgat ctacgatcag ttcatcatgc cccgccgtaa cggccccacc ctgctggcaa
    21841 ttcctttgct ccggcgtggt cgcatcgata gcgttatctt cgtcggattg attgtcattc
    21901 ttatctataa caacgtcacg aatcatggcg cgctaataac aacatggtta ttaagtgcac
    21961 tggctctgat gggtttttat atattctgga tccgcgttcc gaagatcatc tttaaacaaa
    22021 aaggtttttt cttcgccaat gtctggattg aatatagccg aatcaaagcg atgaacttgt
    22081 cggaagatgg cgtgttggtg atgcaattag aacagcgtcg gctgttaatc cgcgttcgaa
    22141 atatcgacga tctggaaaaa atttataagc ttctcg
//
LOCUS       DADLSB010000054        21738 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.054, whole genome shotgun sequence.
ACCESSION   DADLSB010000054 DADLSB010000000
VERSION     DADLSB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.054"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..798)
                     /locus_tag="JN331_002029"
     CDS             complement(<1..798)
                     /locus_tag="JN331_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7260180.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHG
                     AQLLDRLLEPGDIVGFSWGRAVSALVENLPQAGQSQQLICVPIIGGPSGKLESRYHVN
                     TLTYSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSP
                     AIRDGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEK"
     gene            1146..2018
                     /gene="rluF"
                     /locus_tag="JN331_002030"
     CDS             1146..2018
                     /gene="rluF"
                     /locus_tag="JN331_002030"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY7260181.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2151..2423)
                     /locus_tag="JN331_002031"
     CDS             complement(2151..2423)
                     /locus_tag="JN331_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY7260182.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLNNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(2676..4025)
                     /gene="lysC"
                     /locus_tag="JN331_002032"
     CDS             complement(2676..4025)
                     /gene="lysC"
                     /locus_tag="JN331_002032"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY7260183.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQAVQKLHSNLFE"
     gene            4550..6199
                     /gene="pgi"
                     /locus_tag="JN331_002033"
     CDS             4550..6199
                     /gene="pgi"
                     /locus_tag="JN331_002033"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY7260184.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQGLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            6661..6903
                     /locus_tag="JN331_002034"
     CDS             6661..6903
                     /locus_tag="JN331_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260185.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            7017..7655
                     /locus_tag="JN331_002035"
     CDS             7017..7655
                     /locus_tag="JN331_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7260186.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            7652..8389
                     /locus_tag="JN331_002036"
     CDS             7652..8389
                     /locus_tag="JN331_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7260187.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRVPQ"
     gene            8389..10485
                     /locus_tag="JN331_002037"
                     /pseudo
     CDS             8389..10485
                     /locus_tag="JN331_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(10532..10810)
                     /locus_tag="JN331_002038"
     CDS             complement(10532..10810)
                     /locus_tag="JN331_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260188.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            10811..10954
                     /locus_tag="JN331_002039"
     CDS             10811..10954
                     /locus_tag="JN331_002039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260189.1"
                     /translation="MFQRIIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            11080..11490
                     /gene="psiE"
                     /locus_tag="JN331_002040"
     CDS             11080..11490
                     /gene="psiE"
                     /locus_tag="JN331_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY7260190.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(11534..13009)
                     /gene="xylE"
                     /locus_tag="JN331_002041"
     CDS             complement(11534..13009)
                     /gene="xylE"
                     /locus_tag="JN331_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HAY7260191.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(13381..14271)
                     /gene="malG"
                     /locus_tag="JN331_002042"
     CDS             complement(13381..14271)
                     /gene="malG"
                     /locus_tag="JN331_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY7260192.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(14286..15830)
                     /gene="malF"
                     /locus_tag="JN331_002043"
     CDS             complement(14286..15830)
                     /gene="malF"
                     /locus_tag="JN331_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAY7260193.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(15984..17174)
                     /gene="malE"
                     /locus_tag="JN331_002044"
     CDS             complement(15984..17174)
                     /gene="malE"
                     /locus_tag="JN331_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY7260194.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            17539..18654
                     /gene="malK"
                     /locus_tag="JN331_002045"
     CDS             17539..18654
                     /gene="malK"
                     /locus_tag="JN331_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY7260195.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            18726..20066
                     /gene="lamB"
                     /locus_tag="JN331_002046"
     CDS             18726..20066
                     /gene="lamB"
                     /locus_tag="JN331_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY7260196.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            20218..21138
                     /gene="malM"
                     /locus_tag="JN331_002047"
     CDS             20218..21138
                     /gene="malM"
                     /locus_tag="JN331_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY7260197.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPSLGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            21367..>21738
                     /locus_tag="JN331_002048"
     CDS             21367..>21738
                     /locus_tag="JN331_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260198.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDN
                     FIYISDIFLNIKQRNEKNHAINMLREVSISNDTISVKFYRNEKIECACNFLMDKDAQG
                     YIDLSDFDLTNCHFKGDVISKV"
BASE COUNT         5455 a         5539 c         5587 g         5157 t
ORIGIN      
        1 tttttcgctc atattcgttt caaccattgc gccgtgaata tcaaaaaagc gcgagcaaat
       61 atcgccagca acctggcggg cattcaggtc gtcactctct tcaccaccat aaaacgcatg
      121 ccagttagcg ccgtcgcgaa tggccggtga gccaattccc accagggcga catccagatt
      181 atcccagtag gcagagatgg ttttaaagtg ctgagactgc atgatcccat tacgaattaa
      241 tgggttatcc agcagagccg gaaaatccgc gagatgcgat tcccctttca gcttcgctgc
      301 cgcgctgtag gttaatgtgt ttacatgata gcggctttcg agtttaccgg acggtccgcc
      361 aataattggt acgcagatta actgctgcga ttgccccgcc tgcggcaagt tttcaaccag
      421 tgcgctcacc gcgcgacccc aggaaaaacc gacaatatcg ccaggctcca gcaagcgatc
      481 cagcagttgc gcgccgtgta accccatcat cgccagttga gtctcttcat cctcatcatt
      541 tcccgacacc accacaacgt ctttcaggcc aaacttttgc ttcagttgct gctccagcca
      601 gagattttcg ttgtagtcat agtagtcata gttgatggcg atggtgacca ttccctgatc
      661 gcggcctcgt ttaagcaagc ggctgatggt ggtgcggtaa atacccagtt cgcgcgcgat
      721 ttgcgcctgc gtcatatcct gttcgtaata gagttgggca atcttcacaa tcaaacggat
      781 atcgtcactg ttttccattg ccgcctcctg gttctgcaca tttgtgcaaa tacgctttct
      841 caccgcttga tctgatataa ccccatcatg agggcctttc aaagcgattt ttcactcttt
      901 gcatggggag aaatagcgct ttctcttgca catttgtttc tctgcgtagt agtcatcagc
      961 acaggtgtgc agcgattcgt aatatgttga ttatccttat tttgccaggc aaatttgcat
     1021 cgtacttccg gagacacttt tgtgataaaa ggcgtggcag atcacattta atttcgacgt
     1081 tgccgcataa ccctgttttt ccccgctaag aggtacggta tattccgcgt tttacaggag
     1141 aatttatgct gcccgactca tcagtccgtt taaataaata catcagcgaa agcggaattt
     1201 gctcacgccg cgaagcggat cgctatatcg agcaaggcaa tgtgttcctt aatggcaagc
     1261 gagccaccat tggcgatcag gtgaaacccg gcgacattgt gaaagtaaac ggtcagttga
     1321 ttgagcctcg ggaagccgaa gatttggtac ttatcgccct gaacaagccc gtcggtattg
     1381 tcagcaccac cgaagatggc gagcgcgata acattgtcga tttcgttaac cacagcaaac
     1441 gcgtgttccc gattggccgc ctggataaag actcccaggg gctgattttc ctcaccaatc
     1501 acggcgatct ggtgaataag atcctccgtg cgggtaacga tcatgagaaa gagtatctgg
     1561 tgacggtcga taaaccgatt accgatgaat ttattcgtgg tatgggggcg ggggtgccga
     1621 tcctcgggac ggtgacgaaa aagtgcaaag tcaaaaaaga agcgccgttt gtcttccgca
     1681 ttaccctagt gcaggggctg aaccgtcaga tccggcgcat gtgcgagcat ttcggctatg
     1741 aagtgaaaaa gctggaacgt acgcgcatta tgaacgtcag cttaagcggc attccgctgg
     1801 gggagtggcg cgatttaacc gacgatgagt taatcgacct ctttaagctc atcgaaaatt
     1861 cctcttccga ggcaaaacct aaagcgaagg ccaaaccgaa aacagcgggc atcaaacgtc
     1921 cagtcgttaa gatggaaaaa acggcggaaa aaggcggtcg cccggcgtcc aacggtaagc
     1981 gttttacctc gccggggcgt aaaaagaagg gacgctgatt aacgcctcag actgatgaaa
     2041 acgtccaaaa agagctcgga cagtcccctc gccccttcgg ggagagggtt agggtgaggg
     2101 gaagattgtg tccacacaga cgagtgaaaa tctcaggcgt tgattgttca ttagcgcctg
     2161 ccgcgcgtcg acgtatttgc tgaccagcta aatgacgctt caggatccgg tttataagcc
     2221 tgctttttct tcaattggcg ggcttttttc gcttcagcct cacgctcgac cattaattta
     2281 tcgatgtact ctttcttaat gctgttggtt tcggagttgt tcagtacgcg accatgcgca
     2341 atccgcgccc gatccagcag tgttttaagt tcacgctgct cgcgttcagt catctctttt
     2401 tgggtaatac gggggagtgc catgatggtg ccctcagtga gccagagagt tcagtgtacg
     2461 ggaattcgtt tgcgagcaga actgataata ttcattttgc cttctcttaa ctatttatct
     2521 ctttcgataa ttaattagag atgcagataa aaaaattaaa ggcaattatt ctccgataaa
     2581 gacgttggtt aacagagtac aggctcgttt attgatgagc atagtgacaa gaaaatcaat
     2641 acggcccgaa atatagcttc caggccatac agtatttact caaacaaatt actatgcagt
     2701 ttttgcaccg cctgctcggc atcttcgccg ggcaccagga agcacaggtt atggctggat
     2761 gcgccgtaac aaatcatgcg aatgttgaac ggttccagta cgccgaatac ctctttgcca
     2821 acgccgcagg cttttgacag gtcattgcca atcaacgcga ccagcgccag accttcttcc
     2881 acctccaccc ggcacagtgc ggaaagctcc atcagcagag attgcgtcag caacgtatcg
     2941 ccagtggagg ttgaaccggt ggtatcaagg gttaatgcca cgctcacttc tgacgtggtg
     3001 attaagtcta ccgaaatatt atgccgcgcg aggatgccga aaacttccgc gaggaagccg
     3061 cgagaatgca gcatattcag gctgtgcaaa gtgagcagag tctgattgcg acgaagcgcc
     3121 agcgcgcgga acagcggcgg attttcagtt ttattgcaca ccagcgtacc acctgcgcgt
     3181 gggtctttgc tggagccgac aaagaccggg atatcgctgc gtactgcggg tagcaacgtt
     3241 gccggatgca gtacttttgc accaaaagtt gccatctctg ccgcttcggc aaacgcgatt
     3301 tcatcaatgc gttttgctgc ggaaactacg cgtggatcgg tggtgtagat gcccgggaca
     3361 tcggtccaga tatcaacacg agatgcgtgt aaagcctccg ccagcaaggc tgccgtataa
     3421 tcgctgcctc cacggccaag cgtcgttgta cgacctttat tttcgctacc gataaatccc
     3481 tgggtgatca ctaagccttc attgaggcgt gggagcagct gcagcgcggc cagttccgcc
     3541 agcgcggcta catctggctc tgcacgacca aatcggtcgt tggtacgcat cactttacgt
     3601 acatcaaacc actgtgcctg aacatcgcgt tcgcgcagga tttcgacaaa cagcagggtc
     3661 gacatcagct cgccgtggct gaccagctca tctgtcagcg ccggagacgt tgccagcgcc
     3721 gccgcttctg ccagaacagt aatgttctcc agcagacgtt caatctcttc acggataacg
     3781 ttcgggtaac gcagacgttc cagaatggca aactggatgt tgcgaatagc gtcgagtttt
     3841 tcgaatcgct cgccaggttc cagcccttcc gctaaagcga ccagcagatt agtgatacca
     3901 gcagaagccg agaggactac taaacgcacg ttggcatcag aaagcacaat atcagcgctg
     3961 cggttcatgg cgtcaaaatc agctacgctg gtaccgccaa atttggagac aacaatttca
     4021 gacataacta cctcgtgtca ggggatccat tttcagcctt ggcacaaggg aagagcggaa
     4081 gacgggtggg cgcagagcga tacttcgcta ctattttcac ccagaagtgc tccaccactt
     4141 gcgaaacgcc cgactgcgaa cgcttctggt gacaacccag gggattcagc ccctgtagcc
     4201 gatgatgaac gtggccagcc gttcaatcac ctcggcgatg caccccctca ggtgttatca
     4261 caggactggc tcctccaaca ccgttacttg ggcaacgcgc ctcttctggc ctgcgctagc
     4321 gcaggtagta catttataaa taaagggtga gcggggcggt tgtcaacgac ggggtcatgc
     4381 ggatttttca tccactcctg gcggtcagta gttcagctaa taaatgcttc actgcgctaa
     4441 gggtttacac tcaacattac actaacggca ctaaaaccat cacatttttc tgtgactggc
     4501 gctacaatct tccaaagtca caattctcaa aatcagaaga gtattgctaa tgaaaaacat
     4561 caatccaacg cagaccgctg cctggcaggc actacagaaa cacttcgatg aaatgaaaga
     4621 cgttacgatc gccgatcttt ttgctaaaga cggcgatcgt ttttctaagt tctccgcaac
     4681 cttcgacgat cagatgctgg tggattactc caaaaaccgc atcactgaag agacgctggc
     4741 gaaattacag ggtctggcga aagagtgcga tctggcgggc gcgattaagt cgatgttctc
     4801 tggcgagaag atcaaccgca ctgaaaaccg cgccgtgctg cacgtagcgc tgcgtaatcg
     4861 tagcaatacc ccgattctgg ttgatggcaa agatgttatg ccagaagtca acgcggtgct
     4921 ggagaagatg aaaaccttct cggaagcgat tatctccggt gagtggaagg gttataccgg
     4981 caaagcaatc actgacgtag tgaacatcgg gatcggcggt tctgacctcg gcccgtacat
     5041 ggtgaccgaa gcactgcgtc cgtacaaaaa ccacctgaac atgcactttg tttctaacgt
     5101 cgatgggact cacatcgcgg aagtgctgaa aaaagtaaac ccggaaacca cgctgttcct
     5161 ggtagcatct aaaactttca ccactcagga aaccatgacc aacgcccata gcgcgcgtga
     5221 ctggttcctg aaagcggcag gtgatgaaaa acacgttgcg aaacattttg cggcgctctc
     5281 aaccaatgcc aaagccgttg gcgagtttgg tattgatact gccaacatgt tcgagttctg
     5341 ggactgggtc ggcggccgtt actctttgtg gtcagcgatt ggcctgtcga ttgttctctc
     5401 catcggcttt gataacttcg ttgaactgct ttctggcgca cacgcaatgg acaagcattt
     5461 ctccaccacg cctgccgaga aaaacctgcc tgtcctgctg gcgctgattg gcatctggta
     5521 caacaatttc tttggtgcgg aaactgaagc gattctgccg tatgaccagt atatgcaccg
     5581 tttcgcggcg tacttccagc agggcaatat ggagtccaac ggtaagtatg ttgaccgtaa
     5641 cggtaacgtt gtggattacc agactggccc gattatctgg ggtgaaccag gcactaacgg
     5701 tcagcacgcg ttctaccagc tgatccacca gggaaccaaa atggtaccgt gcgatttcat
     5761 cgccccggct atcacccata acccgctctc tgatcaccac cagaaactgc tgtctaactt
     5821 cttcgcccag accgaagcgc tggcgtttgg taaatcccgc gaagtggttg agcaggaata
     5881 tcgcgatcag ggtaaagatc cggcaacgct tgactacgtg gtgccgttca aagtgttcga
     5941 aggtaaccgc ccgaccaact ccatcctgct gcgcgaaatc actccgttca gcctgggtgc
     6001 gttgattgcg ctgtatgagc acaaaatctt tactcagggc gtgatcctga acatcttcac
     6061 cttcgaccag tggggcgtgg agctgggtaa acaactggca aaccgtattc tgccagagct
     6121 gaaagatgat aaagaaatca gcagccacga tagctcgacc aatggtctga ttaaccgcta
     6181 taaagcgtgg cgcggttaat catcatgcgt gcctgatgcc gatatgtagg ccggataagg
     6241 cgtttacgcc gcatccggca accggagcct gatgccaata tcgacattaa tgcatagtat
     6301 gaggagttaa ataaccttaa aaacaatgcc gacaataagt cggcattgtt ttatcagata
     6361 aatccccttg tctgtgattt aacggaaatc ataccgtgag gttaatccta aaatagattt
     6421 ttaatcgttg tttatttcgg agaatacgca gattaattac ttttgttttt attttaagtt
     6481 tatgattttt attgttattt aaatataagt tgaaacttat atttgatatt cattccaatt
     6541 atcctaaaac tccatcgcta attccccgcg ccgtaattcg catgctttag ttgtgtatac
     6601 tcgatcccgc ccgaaatgtt tttgggtaaa tctccattca ttcaatgaag ggaaattgtt
     6661 atgaaaaaag ttctgtatgg catttttgcc atatctgcgc ttgcggcgac ttctgcgtgg
     6721 gctgcacctg tacaggtggg cgaagcggca gagtcggcag caacgtcggt ttcggcgggg
     6781 agttcctccg cgaccagcgt cagcaccgta agctcggcgg tgggtgtcgc gctcgcggca
     6841 accggtggcg gtgatggttc taataccggg accaccacaa ccacgaccac cagtacccag
     6901 taataaagta tgtatcccca aaataatttg agtcattgca tctgtggctt gaagtatgaa
     6961 gggatttacc ataaccacac tccggtgtgg ttattctgcc cctctggaga agagtcgtga
     7021 agcgacctgc actcattctt atctgcctgc tattacaggc ctgttcagcc acgactaaag
     7081 agctgggcaa ttcactgtgg gacagtctgt tcggcacgcc aggcgtacag ctgacggacg
     7141 atgatattca aaatatgccc tacgccagcc agtacatgca gcttaatggc gggccgcaat
     7201 tgtttgtggt gctggccttc gctgaagacg gacaacaaaa atgggtcact caggatcaag
     7261 ccactcttgt cacacaacat ggtcgtctgg tgaagacttt gcttggcggc gacaacctga
     7321 ttgaagtgaa taaccttgcc gccgacccgc tgattaaacc cgcacaaatt gttgatggtg
     7381 caacatggac gcgcacgatg ggctggaccg agtaccagca ggtacgctac gccaccgcac
     7441 gctcagtctt caaatgggat ggcaccgata ccgtcaaggt cggcagcgat gaaaccctgg
     7501 ttcgcgtgct ggacgaagaa gtctccaccg accaggcgcg ctggcataac cgctactgga
     7561 tcgacagcga agggcaaatt cgccagtcgg aacagtatct cggcgcggat tatttcccgg
     7621 tgaaaaccac tctcatcaag gcggcaaaac aatgattaaa caaactattg tcgcgttgct
     7681 tttgagtgtg ggagcgtcat cggtcttcgc ggcaggaacc gtcaaggtgt tcagcaatgg
     7741 cagcagtgag gccaaaacgc taacgggcgc agagcattta atcgatctgg tagggcaacc
     7801 tcgactggca aacagctggt ggcctggtgc ggtgattagc gaagagctgg caacggcggc
     7861 agcattgcgt cagcagcagg cgttgctgac acggctggca gaacagggcg cagattccag
     7921 caccgacgat gccgccgcca ttaacgcctt acgccagcaa atccaggcat tgaaagtgac
     7981 aggcaggcaa aaaatcaatc ttgatcccga tatcgtccgc gttgccgaac gcggtaaccc
     8041 gccgttgcag ggcaactaca cgctgtgggt cggaccaccg ccgtccacgg tcacgttgtt
     8101 cgggcttatc agccatcctg gcaatcagcc attcactcca ggtcgcgacg tggcgagcta
     8161 tctctctgac cagagcctgc tcagcggtgc ggatcgcagc tacgcgtggg tcgtttaccc
     8221 ggacggacgc acgcaaaaag cgccggtggc ttactggaac aagcgtcacg tagagccgat
     8281 gcccggcagc attatttatg ttggcctcgc agactccgtc tggagtgaga cgcctgatgc
     8341 ccttaacgcc gacattcttc agactctgac gcagcgggta ccccaataat gaaaaaaaga
     8401 catctgctta gcttactggc gctgggcatt agcacagctt gctacggcga aacatatcct
     8461 gcgcccattg gtccgtcgca gtcggatttc ggtggcgtag gattattaca aactcccacc
     8521 gcgcgtatgg cacgggaagg ggagttgagt ctgaactatc gcgataacga tcagtaccgt
     8581 tattactcag cttcagtgca actcttcccg tggctggaaa caacgctgcg ctacaccgac
     8641 gtgcgcaccc ggcagtacag cagcgtcgaa gcgttctctg gcgatcaaac gtataaagat
     8701 aaagccttcg atctcaaact gcgtttgtgg gaagagagtt actggctgcc gcaagtggcg
     8761 gttggtgcgc gggatatcgg cggtacgggg ctgtttgatg cggaatatct tgttgccagc
     8821 aaagcctggg ggccgttcga ttttacgctc ggcctgggct ggggatatct gggcaccagc
     8881 ggtaatgtaa aaaatccgct ctgttcagcc agtgataaat attgctatcg cgataacagc
     8941 tacaaacagg cgggatctat cgacggtagc cagatgttcc acggtcctgc ctcactgttt
     9001 ggcggcgtgg aataccagac gccttggcaa ccgctgcgtc tgaaactgga gtatgaaggt
     9061 aataattatc agcaggattt tgccgggaag ctggagcaaa aaagtaagtt taacgtcggg
     9121 gcgatttatc gcgttatcga ttgggccgac gttaacctta gctatgaacg tggcaacacc
     9181 tttatgtttg gcgtcacgct gcgcaccaac tttaacgatc tgcgcccgtc ctacatcgat
     9241 aacgcccgcc cgcaatatca accgcagccg caggatgcca ttttgcagca ttcggtggtg
     9301 gcgaatcagt taacgttgca gaaatacaac gccggacttg ccgatccgca gatccaggcg
     9361 aaaggcgata cgctgtatgt taccggcgag caggtgaaat atcgtgattc gcgcgaaggg
     9421 atcatccgtg ctaatcggat cgtgatgaac gatctgccgg atgggatcaa aacgatccgc
     9481 attacggaaa atcgccttaa catgccgcaa gtgacgacgg aaaccgatgt cgccagcctg
     9541 aaaaatcatc tcgccggaga gccgttgggc cacgaaacga cgctggcgca caaacgcgtc
     9601 gagccagtgg ttccgaagtc caccgagcag ggctggtata tcgacaaatc acgctttgat
     9661 ttccatatcg atccggtgct gaaccagtcg gtcggtggcc cggaaaactt ttacatgtat
     9721 cagctgggcg tgatgggaac ggcagatttg tggctgacgg accatctact gaccaccggc
     9781 agcctgtttg caaatcttgc caacaactac gacaagttta actacaccaa tccaccgcag
     9841 gactcgcact taccgcgcgt tcgtacccat gtgcgcgagt atgtgcagaa cgatgtctat
     9901 gtgaataacc tgcaagccaa ctacttccag catctgggca acggcttcta cggtcaggtc
     9961 tatggaggtt atctcgaaac catgtttggc ggtgcggggg ccgaagtgtt gtatcgtccg
    10021 ctggacagta actgggcgtt tggtctggat gccaactacg ttaaatagcg cgactggcgt
    10081 agtgcaaaag atatgatgaa attcaccgac tacagcgtga aaaccggaca tctgaccgcc
    10141 tactggacgc catctttcgc ccaggacgtg ttggtgaaag ccagcgtcgg gcagtatctg
    10201 gcaggggata aaggcggcac gctggagatc gctaaacgct ttgacagcgg cgtggtggtg
    10261 ggtggctatg ccacgatcac caacgtttcg aaagaggagt acggcgaagg ggacttcacc
    10321 aaaggcgtgt atgtctcggt accgttggat ctcttctcgt ctggcccgac acgcagccgt
    10381 gcggcgattg gctggacgcc gctgacgcgt gacggtggtc agcaacttgg gcgtaagttc
    10441 cagttgtatg acatgaccag cgaccgtagc gtaaatttcc gctaagtcat gggaaaggtg
    10501 ccagttttcg cactcactgg caccctcatt cttaatgacc tgttgagcca tcaggaacgc
    10561 agttcacccc cggtttacga tctggtgtgt aaaggcgaaa acattctgtc ttcctgtccg
    10621 tcgatatcgg ttgcaggtct ggttgcctga gcgactgggc catcattggg ctggagacat
    10681 tgctttgcgg taggtcggct ttatcagcag taccgagcgg accagcataa gcaggaagaa
    10741 cagagactga taacatcaaa gcagcaaaat aaggcttcat ttttaccacc tttatcaggt
    10801 tacgtttcat ttgttccaga ggatcattgt cgatttttcg cgcattgctg gtggctggga
    10861 atcacctgaa tgggtgattt ttgaattacc ggctttggtg cggtttgtct tgccggatgc
    10921 gccgccaggc gcggcttatc cggcctacgg gtaggtatat ccggctttgg tggaggcgcg
    10981 ctccaaatcc aggttgaaca aaacatacac aaaaaatata gatctccgtc acatttttgc
    11041 gttatacagg aagctcgcca ctgtgaagga ggtactgcta tgacgtcact ctctcgtccg
    11101 cgcgtggagt ttatctccac cattttgcag accgtactca atcttggcct gctgtgcctc
    11161 ggcctgattt tggttgtctt cctcggcaaa gaaacggtgc atctggctga tgtgctgttc
    11221 gcgccagaac aaaccagcaa atatgagctg gtagaaggac tggtggttta ctttctctat
    11281 ttcgaattta tcgcgctgat tgtgaagtac tttcagtccg gttttcactt cccgctacgt
    11341 tactttgtct atattgggat caccgccatt gtgcgcttga tcatcgtcga tcataaatcg
    11401 ccactggatg tgttgatcta ctcggctgcg atcctgttac tggtgatcac cctgtggctg
    11461 tgtaattcga aacggctgaa gcgggagtaa aaagacagca cgccgaaatg gcgcggcgtg
    11521 ctggacagga agattacagc gtagcagttt gttgtgtttt cttcgtttcc ggttcccaga
    11581 gcgcttccag ctcctcaagg gttttacctt tggtttccgg gacaaatttc cacataaaca
    11641 gtgctgccag aacgcccata caaccgtaaa tccagtagga gaaaccgttg tggaaatggg
    11701 ccaccagcca ggagtttttg tccatcatcg ggaaggtcca ggagacgaag tagttcgcca
    11761 gccactgggc cgccaccgcg attgccagcg ctttaccacg aatagcattc gggaagattt
    11821 ccgacagcag tacccagcat accggacccc aggacatggc aaaggcggca acatagaaca
    11881 gcatcgacag tagcgccaca atacccgatg cctgagtgta aaacgcggta ccgaggctaa
    11941 acataccgat tgccattccg agtgcgccga taatttgcag tggcttacga ccaaatttat
    12001 ccacagtcat aattgccagt acggtaaagg tgaggttgat aactccgaca ataatggtct
    12061 gcaacagcgc gatatccgtg ctggccccca gcgttttgaa cacttccggc gcgtagtaca
    12121 gcaccacatt gatgccgaca aattgctgga agatggagag cataacgccg attacaatca
    12181 cgcccacgcc aaacatcagc agacgaccac cggttttgcg gccatgatcc agggagtgtt
    12241 taatttcctg tactgcctga gttgcaagcg tgttgcccat aattttgcgc aggatacttt
    12301 ccgcctgttc ttgcttgccg cgtgacatca gccagcgagg actttctggc acggtataca
    12361 gcagcattaa gaacagcagt gcagggatac attccgaggc aaacatataa cgccagccgt
    12421 cagtattcag ccagctggca tcaccggaac gggcaataaa atagtttacg cagtaaacta
    12481 aaagttgccc gaaaataatc gcaaactggt taaatgaaac cagttttccg cgaatatgag
    12541 ctggagccag ttccgcaata tacattggtg aaagcattga ggctaaacca acgccaatac
    12601 cgccaataat acgataaata acaaattccg ggacataacc tgccagataa ataggtactg
    12661 tgttgtccgg gtttatagag gtaaaaccaa gttctggcca ggcagaacct acaccagaaa
    12721 taaaaaacag gacagcagaa atcttaagtg aatcacgacg gccgaagcgg ttactgcaat
    12781 aaccaccgag ggcaccgccg atgatgcaac caatcagagc gctggctacg caaaatccta
    12841 acagggagtt ggcagcggat tcacttaagt tttgtggagc aacaaagacg gtattgagtg
    12901 actcaacagt accggaaata acggcggtgt cgtagccaaa taataaacca cctaatgtag
    12961 cgactaaggt aatcgaaaat atataactgg aattatactg ggtattcatt cagacctgcc
    13021 ttagaccatt ctgatctttt atcagtgcat tgatgttgga attgtcaggc agttaaggca
    13081 attatcattt tttacaatgc gtttacgtaa tgtcttttct gtgatcttaa ttgtgataat
    13141 tatcaaaaat tgatgaaaac aatactattg ccgtgactca gagcacgaaa gagaattatc
    13201 gtaagtggga aaacaaataa cgtaaaaata ataagttcta tgatgggaat attaaccggc
    13261 gaacgattca gattgcagac gaaagaaaaa aaggcgctcc gtggagcgcc gaataatagt
    13321 cacaagttgg gataacgtaa gttgagggtg cagcggcata acattggcag aacaacatct
    13381 ttaacctttc acaccacctg ccgtcaggcc gttgaccagc cagcgttgag ccagcaagaa
    13441 gacgatggtg atcggtaatg cagacatcac ggcagcggcg gcaaagtcac cccacaggta
    13501 gttttgcggg ttgaggtatt gctgcatccc cacggccagg gtgtagctgt ttacgtcacg
    13561 cagtaacagc gacgcgaccg gaacttcagt aatggcagcg ataaacgaca ggatgaatac
    13621 caccgccaga atcggtactg acagcggcaa caggacaagg cggaaggcct gccacggtgt
    13681 cgcaccatcc agcgcagccg cttcttccag cgaactgtcg atggtttcga aatagccttt
    13741 gatggtccag acatgcagcg caatcccacc cagatacgcg aaaattacgc cgccgtgagt
    13801 attcaggcca atgaatggaa tgtactcacc cagacgatca aacaacgcat acaacgcgac
    13861 cagtgaaagt actgccggga acatctggaa aatcagcatt cctttcagca gcgtcgcttt
    13921 gcctggaaag cgcatacggg cgaaagcgta ggcgcaggtg gtggagagcg ccacaatgcc
    13981 aatcgcggaa atcccagcga cctttaccga gttccacagc cacagcagta ccgggaatgg
    14041 cggtggcgta atgcgaccat cagcctgttc aacgctaaaa cctaacgcca gtttccagtg
    14101 atcccaggag atttgctccg ggatcaggct accggtcgcg aagtttccct gacgcagcga
    14161 gatagcgacg accatcagca gcgggaacat aatcgccgcg ataaaaagta gcagtagcag
    14221 gtgagtaata aacaaacgtg ctttttgcga tttcggttgg accattgcca tttttgttat
    14281 ctcccttaat caaacttcat tcgcgtggct ttcaggttca ctatcgccag cgcacccacc
    14341 agcaggaaga tcagcgtggc aattgctgcc gccagaccga agtcctgacc cccgccgcct
    14401 tcaaaagcga tacggtaggt gtagttaaca agcaggtcgg tataaccggc tggcgtggtc
    14461 gtgccaagac gatccgggcc gccgttggtt aacagctgaa tcagcacgaa gttgttaaag
    14521 ttaaaggcga agctggcgat catcagcggc gtcagcggtt taatcagcag cggcagcgta
    14581 atcttaaaga agttctggaa cggacctgcg ccatccattg ctgaggcttc atacaaatcg
    14641 tccggaatcg ctttcagcaa gcccatgcag aggatcatca tgtacggata acccagccag
    14701 gtattgacga taatcagcat cgtgcgagcg gtggtcggat cgctgaacca ggcgggcttc
    14761 acgccaaaca gcgcgctcaa catcatgttg atttcaccga agctctggtt aaacaatcct
    14821 ttgaaaatca agattgaaat gaacgatggc accgcgtagg gcagaatcag caggacgcga
    14881 tagaccgctt tgccgcgcaa cgcttcccac tgcaccagac atgccagaac catgccgacc
    14941 gccaccgtta aaaagacagt gatcagcgag aacaccacgg tccagacgaa aatggcgagg
    15001 aacggtttct gaatgccttc gtcggtaaag acgcgggtaa agtttttcca gccggtggtc
    15061 acggtgtaac cggggcttag cttttcatcc ccccagttgc cgtcggcggt aatggactgg
    15121 taaaagccaa tttggttatt cggacgatat ttcacgccac tctgattatt cgtcaacgtg
    15181 ccgtcaccgt cgagtgtgta gagcggctgc gtgccagaaa actggcgcag ggagctcatc
    15241 atcactttgt tgccatccgg cagaatggcg gtaatgtcac tcagcgcctg acgattctgg
    15301 gtaatcacgc gcagattcgc gcgttcgcct tcgggctggg cggtcgtttc tttcagttgc
    15361 agtttttgct cgccgccaaa tttaaacgcg tcggagaggt aatttttgcc ggtttcgccg
    15421 tcgctgagcg ccagttgcca ctcatcgccc gccgggtaaa gaccaaagtt ataggttttg
    15481 cctgcttgcc aggagcgatc taacaacact tcctgcgcac gttcaaaagt cagctggtta
    15541 gtgctgctgt agttggtgaa ggcaatggcg atggtgcaga ccagagggaa gaggacgaat
    15601 aatcccattc cagccattcc cgggtaaaca tagcgccagg cgtaggcttt acgattggcg
    15661 aaaatataca gccccgctga actcaatatc agcgtggtaa tggcgaacag gtattcccct
    15721 tgtgcgtaca ttaaaacaac aaggtaaccc accagcaggc cgagcagacc tagcactgac
    15781 catttcagcg cgtcgctttg ccaccaatgt ttctttttaa tgacatccat ggggttcttc
    15841 ctcattccag gacggataag gctttcacgc cttatccgac aacaactgcc tgatgcgaca
    15901 ctgatgcgtc ttatcaggcc tacatgcgtt tcagttttgt aggccggaca aggcgttcac
    15961 gccgcatccg gcatttcaca gcattacttg gtgatacgag tctgcgcgtc tttcagggct
    16021 tcatcgacag tctgacgacc gctggcggcg ttgatcaccg cagtacgtac ggcataccag
    16081 aaagcggaca tctgcgggat gttcggcatg atttcacctt tctgggcgtt ttccatggtg
    16141 gcggcaatac gtggatcttt cgccaactct tcctcgtaag acttcagcgc tacggcaccc
    16201 agcggtttgt ctttattaac cgcttccaga ccttcatcag tcagcagata gttttcgagg
    16261 aactctttcg ccagctcttt gttcggactg gcggcgttaa taccggcgct cagcacgcca
    16321 acgaacggtt tagatggttg acccttgaag gtcggcagta ccgttacacc ataattcact
    16381 ttgctggtgt cgatgttgga ccatgcccac gggccgttga tggtcatcgc tgtttcgcct
    16441 ttattaaagg cagcttctgc gatggagtaa tcggtgtctg cattcatgtg tttgttttta
    16501 atcaggtcaa ccaggaaggt cagacccgct ttcgcgccag cgttatccac gcccacgtct
    16561 ttaatgtcgt acttgccgtt ttcatactta aacgcataac ccccgtcagc agcaatcagc
    16621 ggccaggtga agtacggttc ttgcaggttg aacatcagcg cgctcttacc tttcgctttc
    16681 agttctttat ccagcgccgg gatctcttcc caggtttttg gcgggttcgg cagcagatct
    16741 ttgttataaa tcagcgataa cgcttcaaca gcgatcgggt aagcaatcag cttgccgttg
    16801 taacgtacgg catcccatgt aaacggatac agcttgtcct ggaacgcttt gtccggggtg
    16861 atttcagcca acaggccaga ttgagcgtag ccaccaaagc ggtcgtgcgc ccagaagata
    16921 atgtcagggc catcgccagt tgccgcaacc tgagggaatt tctcttccag tttatccgga
    16981 tgctcaacgg tgactttaat tccggtatct ttctcgaatt tcttaccgac ttcagcgaga
    17041 ccgttatagc ctttatcgcc gttaatccag attaccagtt taccttcttc gattttggcg
    17101 agagccgagg cggaaaacat catcgtcgtt aatgcggata atgcgaggat gcgtgcacct
    17161 gtttttattt tcataatcta tggtccttgt tggtgaagtg ctcgtgaaaa cacctaaacg
    17221 gactctagtt tctttatacg gcaacctctt tccatcctcc ttgcccctac gccccaccgt
    17281 tgctttgtgt gatctctgtt acagaattgg cggtaatgtg gggatgcgca cataaaatcg
    17341 ccacgatttt tgcaagcaac atcacgaaat tccttacatg acctcggttt agttcacaga
    17401 agccgcgttc tcatcctccc gcctcctccc ccataaaaaa gccagggggt ggaggattta
    17461 agccatctcc tgatgacgca tagtcagccc atcatgaatg ttgctgtcga tgacaggttg
    17521 ttacaaaggg agaagggcat ggcgagcgta cagctgcaaa atgtaacgaa agcctggggc
    17581 gaggtcgtgg tatcgaaaga tatcaatctc gatatccatg aaggtgaatt cgtggtgttt
    17641 gtcggaccgt ctggctgcgg taaatcgact ttactgcgca tgattgccgg gcttgagacg
    17701 atcaccagcg gcgacctgtt catcggtgag aaacggatga atgacactcc gccagcagaa
    17761 cgcggcgttg gtatggtgtt tcagtcttac gcgctctatc cccacctgtc agtagcagaa
    17821 aacatgtcat ttggcctgaa actggctggc gcaaaaaaag aggtgattaa ccaacgcgtc
    17881 aaccaggtgg cggaagtgct gcaactggcg catttgctgg atcgcaaacc gaaagcgctc
    17941 tccggtggtc agcgtcagcg cgtggcgatt ggccgtacgc tggtggccga gccaagcgta
    18001 tttttgctcg atgaaccgct ctccaacctc gatgctgcac tgcgtgtgca aatgcgtatc
    18061 gaaatctccc gtctgcataa acgcctgggc cgcacaatga tttacgtcac ccacgatcag
    18121 gtcgaagcga tgacgctggc cgacaaaatc gtggtgctgg acgccggtcg cgtggcacag
    18181 gttgggaaac cgctggaact gtaccactat ccggcagacc gttttgtcgc cggatttatc
    18241 ggttcgccaa agatgaactt cctgccggta aaagtgaccg ccaccgcaat cgatcaagtg
    18301 caggtggagc tgccgatgcc aaatcgtcaa caagtctggc tgcctgttga aagccgtgat
    18361 gtccaggttg gagccaatat gtcgctgggt attcgcccgg aacatctact gccgagtgat
    18421 atcgctgacg tcatccttga gggtgaagtt caggtcgtcg agcaactcgg caacgaaacc
    18481 caaatccata tccagatccc ttccattcgt caaaacctgg tgtaccgcca gaacgacgtg
    18541 gtgttggtag aagaaggtgc cacattcgct atcggcctgc cgccagagcg ttgccatctg
    18601 ttccgtgagg atggcactgc atgtcgtcga ctgcataagg agccgggcgt ttaagcaccc
    18661 cacaaaacac acaaagcctg tcacaggtga tgtgaaaaaa gaaaagcaat gactcaggag
    18721 atagaatgat gattactctg cgcaaacttc ctctggcggt tgccgtcgca gcgggcgtaa
    18781 tgtctgctca ggcaatggct gttgatttcc acggctatgc acgttccggt attggctgga
    18841 caggtagcgg cggtgaacaa cagtgtttcc agactaccgg tgctcaaagt aaataccgtc
    18901 ttggcaacga atgtgaaact tatgctgaat taaaattggg tcaggaagtg tggaaagagg
    18961 gcgataagag cttctatttc gacactaacg tggcctattc cgtcgcgcaa cagaatgact
    19021 gggaagctac cgatccggcc ttccgtgaag caaacgtgca gggaaaaaac ctgatcgaat
    19081 ggctgccagg ttccaccatc tgggcaggta agcgcttcta ccaacgtcat gacgttcata
    19141 tgatcgactt ctactactgg gatatttctg gtcctggtgc cggtctggaa aacatcgatg
    19201 ttggcttcgg taaactctct ctggcagcaa cccgctcctc tgaagctggt ggttcttcct
    19261 cttttgccag caacaatatt tatgactata ccaacgaaac cgcgaacgac gttttcgatg
    19321 tgcgtttagc gcagatggaa atcaacccgg gcggcacatt agaactgggt gtcgactacg
    19381 gtcgtgccaa cctgcgtgat aactatcgtc tggttgatgg cgcatcgaaa gacggctggt
    19441 tattcactgc tgaacatact cagagtgtcc tgaagggctt taacaagttt gttgttcagt
    19501 acgctactga ctcgatgacc tcgcagggta aaggtctgtc gcagggttct ggcgtcgcgt
    19561 ttgataacga aaaatttgcc tacaatatca acaacaacgg tcacatgctg cgtatcctcg
    19621 accacggtgc gatctccatg ggcgacaact gggacatgat gtacgtgggt atgtaccagg
    19681 atatcaactg ggataacgac aacggcacca agtggtggac cgttggtatt cgcccgatgt
    19741 acaagtggac gccaatcatg agcaccgtga tggaaatcgg ctacgacaac gtcgaatccc
    19801 agcgcaccgg cgacaagaac aatcagtaca aaattaccct cgcacaacaa tggcaggctg
    19861 gcgacagcat ctggtcacgc ccggctattc gtgtcttcgc aacctacgcc aagtgggatg
    19921 agaaatgggg ttacgactac aacggcgata gcaaggttaa cccgaactac ggcaaagccg
    19981 ttcctgctga tttcaacggc ggcagcttcg gtcgtggcga cagcgacgag tggaccttcg
    20041 gtgcccagat ggaaatctgg tggtaatagc aaaacctggg ccggataagg cgtttacgcc
    20101 gcatccggca taaaaacagg ttgtcattat ctgaaagggg cgaaagcccc tctgattatc
    20161 gggtttagcg cgctattgcc tggctaccgc tgagctccag attttgaggt gaaaacaatg
    20221 aaaatgaata aaagtctcat cgccctctgt ttatcagcag ggttactggc aagcgcgcct
    20281 ggaattagcc ttgccgatgt taactacgta ccgcaaaaca ccagcgacgc gccagccatt
    20341 ccatctgccg cgctgcaaca actcacctgg acaccggtcg atcaatctaa aacccagacc
    20401 acccaactgg cgaccggcgg ccaacaactg aacgttcccg gcatcagtgg tccggttgct
    20461 gcgtacagcg tcccggcaaa cattggcgaa ctgaccctga cgctgaccag cgaagtgaac
    20521 aaacaaacca gcgtttttgc gccgaacgtg ctgattcttg atcagaacat gaccccatca
    20581 gccttcttcc ccagcagtta tttcacctac caggaaccag gcgtgatgag tgcagatcgg
    20641 ctggaaggcg ttatgcgcct gacaccgtcg ttggggcagc aaaaacttta tgttctggtc
    20701 tttaccacgg aaaaagatct ccagcagacg acccaactgc tcgacccggc taaagcctat
    20761 gccaaaggcg tcggtaactc gatcccggat attcccgatc cggttgctcg tcataccagc
    20821 gatggcttac tgaaactgaa agtcaaaacg aactccagct ccagcgtgtt ggtaggacca
    20881 ctatttggtt cttccgctcc agctccggtt acggtaggca acacggcggc accagctgtg
    20941 gctgcacctg ctccggtgcc ggtgaagaaa agcgagccga tgctcaacga cacggaaagt
    21001 tattttaata ccgcgatcaa aaacgctgtc gcgaaaggtg atgttgataa ggcgttaaaa
    21061 ctgcttgatg aagctgaacg cctgggatcg acatctgccc gttccacctt tatcagcagt
    21121 gtaaaaggca aggggtaatt acgccccaca gtgctgattt tgcaacaact ggtgcgtctc
    21181 ctggcgcacc tttttttatg cttccttcct gggatatgag cgatttttta tagtacttca
    21241 cttcttcttc actaagaatg tccattattt caatgcatta tcagagattc ttttcctttc
    21301 gccggtagtg tctggacgct caggctactt ttccaggtta ttttatttct gtattacaga
    21361 ggttttatga taggtcatat cacaaattct ggcggcaata actctttgat gaaacatgat
    21421 gtggtgcaag gaaataatat agtagatctt gatttactac gtaatttcaa tggggtgaca
    21481 ggtttaaaca gagataactt tatttatatc agtgatatat ttttaaatat aaaacaacgg
    21541 aacgaaaaaa atcatgcaat aaatatgtta cgtgaagttt caattagtaa tgatactata
    21601 agtgtaaaat tctacagaaa tgaaaaaata gaatgcgctt gtaattttct gatggataaa
    21661 gatgcgcagg ggtatatcga cctgtctgat tttgatttaa caaattgtca ttttaaaggt
    21721 gacgttattt ctaaggtg
//
LOCUS       DADLSB010000055        21686 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.055, whole genome shotgun sequence.
ACCESSION   DADLSB010000055 DADLSB010000000
VERSION     DADLSB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.055"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(15..1010)
                     /gene="yjfF"
                     /locus_tag="JN331_002049"
     CDS             complement(15..1010)
                     /gene="yjfF"
                     /locus_tag="JN331_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY7260199.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(997..2019)
                     /locus_tag="JN331_002050"
     CDS             complement(997..2019)
                     /locus_tag="JN331_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7260200.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2033..3535)
                     /locus_tag="JN331_002051"
     CDS             complement(2033..3535)
                     /locus_tag="JN331_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7260201.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQFGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3675..4631)
                     /gene="ytfQ"
                     /locus_tag="JN331_002052"
     CDS             complement(3675..4631)
                     /gene="ytfQ"
                     /locus_tag="JN331_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HAY7260202.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            4941..5471
                     /gene="ppa"
                     /locus_tag="JN331_002053"
     CDS             4941..5471
                     /gene="ppa"
                     /locus_tag="JN331_002053"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY7260203.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="JN331_002054"
     CDS             complement(5851..6192)
                     /locus_tag="JN331_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7260204.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="JN331_002055"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="JN331_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY7260205.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="JN331_002056"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="JN331_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY7260206.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12539
                     /gene="msrA"
                     /locus_tag="JN331_002057"
     CDS             11910..12539
                     /gene="msrA"
                     /locus_tag="JN331_002057"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY7260207.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGVCLPPEA"
     gene            12862..14205
                     /locus_tag="JN331_002058"
     CDS             12862..14205
                     /locus_tag="JN331_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7260208.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14267..14473)
                     /locus_tag="JN331_002059"
     CDS             complement(14267..14473)
                     /locus_tag="JN331_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY7260209.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14798..15352
                     /locus_tag="JN331_002060"
     CDS             14798..15352
                     /locus_tag="JN331_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7260210.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15415..16353)
                     /locus_tag="JN331_002061"
     CDS             complement(15415..16353)
                     /locus_tag="JN331_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260211.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16565..17305)
                     /gene="cysQ"
                     /locus_tag="JN331_002062"
     CDS             complement(16565..17305)
                     /gene="cysQ"
                     /locus_tag="JN331_002062"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY7260212.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17495..19433
                     /gene="cpdB"
                     /locus_tag="JN331_002063"
                     /pseudo
     CDS             17495..19433
                     /gene="cpdB"
                     /locus_tag="JN331_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
     gene            complement(19559..19939)
                     /locus_tag="JN331_002064"
     CDS             complement(19559..19939)
                     /locus_tag="JN331_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7260213.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYSVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20028..20888
                     /gene="qorB"
                     /locus_tag="JN331_002065"
     CDS             20028..20888
                     /gene="qorB"
                     /locus_tag="JN331_002065"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7260214.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            20997..>21686
                     /locus_tag="JN331_002066"
     CDS             20997..>21686
                     /locus_tag="JN331_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7260215.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDCARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRP"
BASE COUNT         5224 a         5812 c         5340 g         5310 t
ORIGIN      
        1 acggagaggc atttttatcg ctgcgcaatg ttgactcttg tcactggcga actctgacga
       61 ttctcccaca gcaccgttaa cccacgctgt aatgctataa aaataaacaa caaaataccg
      121 atggcgattt tcgtccacca ggaactcagc gtgccgtcaa agttgatgta agtctggatc
      181 agtccctgaa ttgccacgcc aaaaagcgtc ccaagcaccg tcccgacgcc accacttaac
      241 aacgtaccgc caatcaccac cgacgcaatg gcgtccagtt ccacacctac gcccgccagt
      301 gcatatccgg cttgggtata aatcgagaag acaatccccg ccagtgttgc cagtccggtg
      361 gagagcatat aaatgcgaat agtggtgctg cgagtagaaa tccccatcag gttcgccgac
      421 gtcgcgttgc cgccaatagc gtacacctga ttgccaaaac gggtacgatg cgcaaggaaa
      481 ataccgataa ccaccaccgc cagcatcaac agccccatcg cactaaggcg accgccgccg
      541 gggattttcc acgcaaggct tgagagcgtg tcataaatgg gatggtttat cggaatcgac
      601 tcttccgaaa cgagatagct gacgccgcgc aaaaagaaca tccccgcaag cgtaatgata
      661 aatgccggga tcttcagggc gtcgatcaga agccccataa atgcgccgaa ggcacagccc
      721 atcaccagca ctagcggaaa cgccagcagc ggcgagaggc cgaaatcgcc aaccactttt
      781 gccagaaaca cgccagtaaa ggcgatcacc gaaccgacgg agagatcgat accgccagag
      841 aggatcacaa aggtcatgcc aacggcgatt atcccaagaa aggcgttatc ggtcaggata
      901 ttgcagatca cccttgtgga agcaaaaccg ggaaactggg tcaggcagta gagatagccc
      961 agcacaaaga cgccgatggt gatcatcagc ggcaagttac gttttatcat ggctacgtac
     1021 tcctttaatc agactgataa agcgttgcga ctgaacaatc agcacacaaa gcaccaccac
     1081 cgctttcacc acctggttca tctccggcgg aaagcccgaa agcaaaattc cggtgttcat
     1141 cccctgaata atcagcgccc ccactaccga gagcagcagg ttaaagcgcc cgcccatcag
     1201 cgatccgccg ccaatcacca ccgcgagaat ggcgtccagc tccagccata atccggcgtt
     1261 gttggcatcg gcaccgcgaa tatccgccgc cacgataatg cccgcaatcg ccgcacatag
     1321 cccgctcaac acgtaggtga gcatgacgat gattcgcgtg tttaccccgg cattttttgc
     1381 cgcccgaatg ttgataccaa cggcttcgat aaacatcccc agcgccgttt tgcgggtcaa
     1441 cagccagaac aggagaagcg tcagcaccgc gataatgacc ggcgttggca ggaacaacag
     1501 cgatccgctg ccaaaccatg agagatccgg cgagttaaac gtgacgatct gcccggcggt
     1561 gatcagttgc gccacgccgc gcccggcgac catcaggatc agggtggcga caaacggctg
     1621 aattttgagg atcgctacca gtatgccgtt ccacaatccc gccaggatgc cggtgcccag
     1681 ggcgcttaac aaaacaatcg gcaggctgaa tcccgcgacc gtcatcgcag ccgttgtggc
     1741 tccggcgata gccatcaccg cccccacaga gagatcgata ccaccggtgg cgatcaccag
     1801 cgtcatacca atcgccagta acgcaacggg agctgcacgg ttaagaatgt cgatagggct
     1861 accgaacaaa cgcccatcct gaagcaccac ctgccagaaa tgcggggcta ccaggctatc
     1921 gaccagcagc accagcaaca gtgccgccag ctgcggcatc cccgtcggcc agtgaaaacg
     1981 cctttttggc ggcgtagtgt cagggagaga ttgaggcatc acactgttct ccttacgccg
     2041 caatggcgtt catgatcgcc ggaacggaaa gctgtgccag cgggatctcc gccacctgtt
     2101 tgcgatcgcg catgatgatc acccggtcgg catagcccac cagctcttcc agttcggagg
     2161 agatcaccag cagcgccaga ccgtcagcac acagcgtttc aatcaggcgg atgatctcgg
     2221 cgtgggcacc aacatcaatg ccacgggttg gctcatcaag gatcagaaat tgcggtcggg
     2281 tcagtagcca acgtgaaagc agcacttttt gctgattgcc accggagaga aattcaatcg
     2341 gttgttcagt tgaaggtgtg cgaatgccaa actggcggat aaagcgttcg gcaatctctt
     2401 gctgctcttt gcgggaaatg ggtcgtagcc agccgcgctg ggcctggaga gcgaggatga
     2461 tattttcccg caccgaggcg gcggcgatga tgccatcggt tttcctgtct tccgggcaga
     2521 atccgatgcc cagtactgaa gcctgatgtg gcgatcgcag ggtttgcggt ttgcctttga
     2581 tcaacgccgt gccgctgtca gcaggtttga taccgaagat cacttcggcg gtttcggtac
     2641 gtccggatcc cagcaagcca gccagaccga cgatctcgcc ggggcgcact tcgagatcaa
     2701 acggtgctat cgttcctttt ttgccgtaat ttttgaacgc ggcaacgggt ttgtcgctca
     2761 acaatgttcg cccggcacgc tgtagcgcgt gggtatccag ctcgcgcccc agcatcattt
     2821 ttaccagttc gatctgcggt agctcgcgcg tttcccgaca gcctacgaaa ctgccgttgc
     2881 gtaagacggt gatccgatcg ctgacctgat agacctgatc gagaaagtga gtgacaaaga
     2941 tcaggctgac gccgcgatcg cgcaactgac gcatcaggcc aaataatagc tccacttcct
     3001 gggtgtcgag gctggcggtg ggttcatcga ggatcagcac tttggcggag agatcgatag
     3061 cccggcaaat ggcgacgatt tgctgcatcg ccaccgaaaa acggttaagc ggttcgcgca
     3121 cgtcgaggga gaaaccgtaa gatgccatca gttcggtggc gcgcttttcc atctctttac
     3181 ggcgtagaag gccgaaacgt ttgggttcgc gacctatgaa tagattatca gcaaccgaca
     3241 tattgggtag caggttgact tcctgataga cggtgccgat accgagttgt tgtgcatggg
     3301 cggtattttt cggtgagata gcctggcctt ccagccagat ggtgccgcga tcggcgtggt
     3361 atacaccagt taatgctttg attagcgttg attttcccgc cccgttttca ccaagcagcg
     3421 ccatgatttc gccacggcgc aggctgaaat caacgttgtc taacgctttg acgccgggga
     3481 aaaatttact taatccttcg gtgcggagga tctcctggtg ttggtcggtc gtcatggttt
     3541 cccccggtgt aaagcgtgct cccagtaggc atgataagac gcggtaagcg tcgcatcagg
     3601 cttcagtgca ccatcgccgg atgcggcgcg agcgccttat ccggcctacg aatcggcatc
     3661 aggcatagca accctcaata ccccatattt ttcttcttct ctaactcttc ttttgcggta
     3721 tcaggcaggt aaagtgtgga tttggttaac gtcagctttt caggcatggt gccgtctttt
     3781 ttgtatttct ccagcgcatc gaaagcgggt cccgccatgt tcggcgtcag ttcaacactg
     3841 gcgttcgctt cgccatccat catcgctttg tagatgtccg gtacaccgtc aatggaaccc
     3901 gtgaggatat ctttgcccgg tttcaggccc gcttctttaa ttgcctgaat tgcaccaatc
     3961 accatgtcgt cgttatgggc gtaaaccatg cagatgtttt tgccgttgtt ttccgctttg
     4021 ataaagctct ccatgacttc tttgccttta ctgcgggtga agtcacctga ctgcgagcgg
     4081 ataattttga tatttggcgc attcttaatg gcttcggcaa agcctttctt acggtcaatg
     4141 gcgacgctgg ccccaacggt gccctgcagc tccaccacgt tgcatggttt gccattcact
     4201 tcttttacca gccagtcacc gatcaactta ccttcgagga tgttgtcggc agtaacggtg
     4261 gtcatataga gagatttgtc tttcacatcg atggaacggt cgagcaagaa gaccgggatt
     4321 tcggcatctt tcgcctcttt taataccggt tcccaacccg tcgcaaccac tggagcgata
     4381 aagatcgcat ccaccccttg tgcaacgaag gaacgtaccg ctttaatctg gttttcctgc
     4441 ttttgctgac catcggcaat tttcaacgta attccgcgtt tttcggcctc acttttcgcc
     4501 acattggttt ctgcggcgcg ccagccggat tccgatccga cctgcgaaaa tcctacggtt
     4561 aatggagcgg ccaacgccat agacgacatg gctgccgaga ctgcagagac tacaagtaag
     4621 cgtttccaca tagggttgtc ctcgtcgggg taattattat tggtgaaaag atgttcgcga
     4681 aaaaactata gacaattcgt tatgtaacgg attgcgttac atcacacttc aaaaaagtaa
     4741 ataaaacgtt aatcacaagt ttgtaatcgc tttcatctca ctatgaaaaa tgcggctacg
     4801 gttatggatt ttcctgctct gtataccgtc ttaaaactgg cgaaaaagga aaatgaagac
     4861 gaaaacaagc aaagacattc ggcgcgagtt ggctataata ctcggcactt gtttgccaca
     4921 tatttttaaa ggaaacagac atgagcttac tcaacgtccc tgcgggtaaa gatctgccgg
     4981 aagacatcta cgttgttatt gagatcccgg ctaacgcaga tccgatcaaa tacgaaatcg
     5041 acaaagagag cggcgcactg ttcgttgacc gcttcatgtc caccgcgatg ttctatccgt
     5101 gcaactacgg ttacatcaac cacaccctgt ctctggacgg tgacccggtt gacgtactgg
     5161 tcccgactcc gtacccgctg cagcctggtt ctgtgatccg ttgccgtccg gttggcgttc
     5221 tgaaaatgac cgacgaagcc ggtgaagatg cgaaactggt tgcggttccg cacagcaagc
     5281 tgagcaaaga atacgatcac attaaagacg taaacgatct gcctgaactg ctgaaagcgc
     5341 aaatcgctca cttcttcgag cactacaaag acctcgaaaa aggcaagtgg gtgaaagttg
     5401 aaggttggga aaacgcagaa gccgctaaag ctgaaatcgt tgcttccttc gagcgcgcaa
     5461 agaataaata agttcttcta gcgcaataac cctgaacgcc gggcttcggt tagtaagggt
     5521 ttttttatgc ccgcgataaa taaactctct attccaccat cattattctc agcggttgca
     5581 aggcttgaac ggtaagaaca agcaaacccg accaccattt tgctgttcat agccacttgc
     5641 tggaagttag ccgacctcac tcatactcac cgttcaaagc gtgcccccgt tttacccctg
     5701 cacatttgtg aacgccagca gagtttttta tgcgattgca gatatggaaa gcggctcgga
     5761 gagaaaagca aaagatggaa aagttacaaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggtggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcatcac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc tacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgaccgccag atgcagatta cccgctttta ccgccacgcc tggctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccggcgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgttct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtcgccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccag cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttaaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcgtctg tctgccgccg gaagcatagc
    12541 gttacgggta caaatgtagt ttgttgataa agtgctcttt gtctatgcct gatgcggctt
    12601 aaacgactta ccaggcctac aaaatcgtcc aaattcaata tattgcaagg actgcgtagg
    12661 cctgatcggc atagcgcatc aggcagtatt gcatttatca gcagtctgaa ttttaaccct
    12721 ctggcgactt tacagtacct tacgctatac tagccactga aaatgccggt tcactttctt
    12781 cgaatcggct ttcaatgtgt atttcacaca aattaatcaa ctttcccttc cgaggatctg
    12841 gcctgaaagg tcggataaga tatgttaaac agtattttag tcatactctg cttgatcgct
    12901 gtaagtgcgt tcttctcgat gtccgagatc tcgcttgccg cctcacgcaa aatcaaactt
    12961 aaactgctgg ctgatgaagg caatataaat gcccaacgcg ttctgaatat gcaggaaaat
    13021 cccggcatgt tctttaccgt ggtccaaatc ggtctgaacg cagtggcgat tctcggcggt
    13081 atcgtcggtg atgcggcatt ttctccagct tttcacagcc tgttctcccg ctatatgtcg
    13141 gcagagctct ctgagcaact gagctttatt ctctctttct cgttagtgac tggcatgttt
    13201 atcctgtttg cggatttaac cccgaaacgc atcggtatga ttgcgccaga agcggtggct
    13261 ttgcgtatca tcaacccgat gcgcttctgc ctgtacgttt gcaccccgct ggtgtggttc
    13321 ttcaacggcc tggcgaacat gatcttccgt attttcaaac tgccaatggt acgtaaagat
    13381 gacatcactt ctgatgacat ctacgcggta gtggaagccg gtgcgctggc gggcgtgtta
    13441 cgtaaacagg aacacgagct gattgaaaac gtctttgagc tggaatcccg taccgttccg
    13501 tcttcaatga caccgcgtga aaacgtgatt tggtttgatc tccacgaaga tgagcaaagc
    13561 ctgaagaata aggtggcgga acatccgcac tctaagttcc tcgtctgtaa tgaagatatt
    13621 gaccacatca tcggttatgt cgattctaaa gacctgctga accgcgtgct ggctaaccaa
    13681 agcctggcac tgaacagcgg cgtacaaatt cgcaacacgc tgattgtgcc ggatacgtta
    13741 accctttcag aggcgttgga aagttttaaa accgcaggtg aagacttcgc ggtgatcatg
    13801 aacgagtacg cgctggtggt ggggatcatc accctcaacg acgtgatgac cacgctgatg
    13861 ggcgatctgg tcggtcaggg gctggaagag cagattgtcg cccgtgatga gaactcatgg
    13921 ctgattgacg gcggcacccc aattgacgac gtcatgcgcg tgctggatat tgacgagttc
    13981 ccgcagtcgg gcaactacga aaccatcggc ggctttatga tgtttatgct gcgtaagatc
    14041 ccgaaacgca ccgattcggt gaaattcgcc ggctataaat ttgaagtggt ggatatcgat
    14101 aactaccgca tcgaccagct gctggtgacc cggatcgaca gcaaggccac cgccctttcg
    14161 ccaaaactgc ctgacgctaa agataaagaa gaaagcgtcg cgtaaccccc agaaacatca
    14221 acggctcctg aatcaggagc cgtcttatta ctgcatagca ctttggttaa gccatctctg
    14281 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    14341 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    14401 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    14461 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    14521 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    14581 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    14641 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    14701 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    14761 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    14821 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa tcggtcagcg
    14881 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    14941 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    15001 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    15061 gttaccgcac gatcgttacc agaccaccac cattgttaac accgacgacg cgattccggg
    15121 ttccggcatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    15181 gtttattgtc gatagcaatg gtgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    15241 cgctgtagtg gtgctggata aagacggtcg cgtgcaatgg gccaaagacg gggcgctcac
    15301 accggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaata
    15361 acataaattc tggaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    15421 ctctgagttc ttacaccgaa ctaatactgg taacgctagt tgctcttttt taaacgcctc
    15481 aatggtcgat ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    15541 attcgcactt actatcgcat ccacttgttc ggctaattcg acttgttggc aacctgcatt
    15601 aaccagaaaa ttataaataa gagagtaatc ttcatcctcc aaaacaacag aggaaccatt
    15661 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    15721 ggcaaaatta tcatcaatta ttttttcgta aatataccat ccatcgcatt ctttaattcc
    15781 accgcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta catcctccaa
    15841 taaggcataa ccgaaagata cgtcaccaaa cgcctggcga atataacctt taatcctttt
    15901 tggtatgtca gactgttttt tctgcaaaat atcacaggca tacaatcttc tgttttgttc
    15961 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    16021 aatttcttcg tattcaaacc cactgcctcg caatgtcatt gtatattcag caccgttatc
    16081 tttaaggctg aaactgataa gaaaattatc catggcatta acagtttcat tttttgatga
    16141 aagcacttga taactatttt tatcaatctg tgttgcgcaa cgaaaggact cccgcaaagt
    16201 taacagacta tcataattcg aaacccaatc ttttttctta aatgcatgct gaatattatt
    16261 aaaaaaattg tttgaatgtg tttttgtatg cgatgacttt acaaataact ccaccgcacc
    16321 aataaaatta ttgtgtctga tcctgggtaa catatggcta ccttatttta tttggttatt
    16381 ttatgtcgcc attaaatatc tcattataga agtaaactat caaaaaacgc tcattattaa
    16441 aatgtaactt attagcaatt gaatttttgc actttaaata caatctcttt ttatttataa
    16501 ctttttaggt tgggataaat caggatggca gaatcaggaa atacactgtt tctgccatct
    16561 gaatttagta aatggacact ctgaaccccg gattcaggaa cgactcgcgc ggagtgtaat
    16621 ccagcggttt accctgccag tcgtgaacgt gcgctccggc agctgcagcc acagcatgtc
    16681 cagcggcggt gtcccaaata ttcgttggtc cgaagcgcgg gtacagctgc gcctgtcctt
    16741 ccgccaccag gcagaatttc agcgaagagc cgatggacgt ggtctgatgt tcgccaagct
    16801 gttgcagata ctctttcagc tccgcatctg catgggaacg gctgataacc accagcggcg
    16861 ggcgcgcatc gcggacctga atcagcttgc gcacaccgca ctcttctttc caggccttgc
    16921 cttctgccgc gctgtacatt acgttcatta ccggcgcata caccacacct aaaatcggtt
    16981 tgccatggtc aatgagcgca atgttaacgg tgaattcgcc attacgttta ataaactctt
    17041 tagtaccatc cagcgggtct accagccagt aacgctgcca gtgctgacgg acttcccaac
    17101 cgggaggatc ttcttcagaa aggaccggaa tatccggtgc cagcgtacgt aaaccgtcca
    17161 tgataacggt gtgagcggca atatccgctg ccgttaccgg agaattgtcc gctttgctga
    17221 cgacgtccat cggtttcgtc ccgtcgtaga cctgcataat ggcatcgcct gcattccgtg
    17281 caagctggca tacttgatct aacatttctc cacctcgtct ctgtgagcgg tgttaactta
    17341 ttgttttact tataccctat cgttaatgaa tgcgccaact gtgatagtgt catcattttc
    17401 aaagcgtaaa attgtggcat tcttcactgt tctataagta aggcgtttat tctccctttt
    17461 ctttcgtatt cccgatgata aaaggatgtc cctgatgatt aagtttagcg caacgctcct
    17521 ggccacgctg attgccgcca gtgtgaatgc agcgacggtc gatctgcgta tcatggaaac
    17581 cactgatctg catagcaaca tgatggattt cgattattac aaagacaccg ccacggaaaa
    17641 attcggactg gtacgtacgg caagcctgat taacgatgcc cgcaatgaag tgaaaaacag
    17701 cgtactggtc gataacggcg atttgattca ggggagtccg ctggccgatt acatgtcggc
    17761 gaaaggatta aaagcaggtg atgttcatcc ggtttataag gcgctgaata cgctggatta
    17821 tacggtcggt acactcggca atcatgaatt taactacggt ctggattacc tgaaaaatgc
    17881 gctggcggga gcgaaattcc cttatgtaaa tgccaacgtc attgacgcca gaaccaaaca
    17941 gccaatgttt acaccgtatt taattaaaga taccgaagtg gtcgataaag acggaaaaaa
    18001 acagacgctg aagattggct atattggcgt cgtgccgccg caaatcatgg gctgggataa
    18061 agctaattta tccggaaaag tgacggtgaa tgatattacc gaaaccgtgc gcaaatacgt
    18121 gcctgaaatg cgcgagaaag gtgccgatgt cgttgtcgtt ctggcgcatt ccgggctgtc
    18181 tgccgaataa agtaatggcg gaaaactcag tttattacct cagtgaaatt ccgggcgtta
    18241 acgccattat gtttggccat gctcacgccg ttttcccggg taaagatttt gctgatatcg
    18301 aaggggctga tatcaccaaa ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt
    18361 ggggcgatca tcttggggtg gtcgacttac aactcagtaa tgacagcggt aaatggcagg
    18421 tgacgcaggc gaaagcggaa gcacggccga tttacgacat cgccaataaa aaatccctcg
    18481 cggcggaaga cagcaagctg gtagaaacac tcaaagccga tcacgatgcc acacgccagt
    18541 tcgtcagcaa gccaatcggt aaatctgccg acaatatgta tagctatctg gcgctggtgc
    18601 aggacgatcc gaccgtgcaa gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca
    18661 ttcagggcga tccggatctg gcaaaactgc cggtgctttc agctgccgca ccgtttaaag
    18721 ttggtggtcg caaaaatgac ccggcaagct atgtggaggt ggaaaaaggc cagttgacct
    18781 tccgtaatgc cgccgatctt tatctctacc ccaatacgct gattgtagtg aaagccagcg
    18841 gtaaagaggt gaaagagtgg ctggagtgct ccgccggaca gtttaaccag attgctccta
    18901 acagcacgaa accacagtca ctcatcaact gggatggttt ccgcacttat aactttgatg
    18961 ttattgatgg tgtgaattat cagattgatg ttacccaacc cgcccgctat gacggcgagt
    19021 gccagatgat taatgccaat gcagagcgga ttaagaacct gacctttaat ggcaagccga
    19081 ttgatccgaa cgctatgttc ctggttgcca ccaataacta tcgcgcttac ggcggcaaat
    19141 ttgccggtac gggcgacagc catatcgctt ttgcttcacc ggatgagaac cgctcggtgc
    19201 tggcagcgtg gattgctgat gagtcgaaac gtgcggggga aattcatccg gcggcagata
    19261 acaactggcg tttagcaccg atagctgccg ataagaaact ggatatccgt ttcgaaactt
    19321 ccccgtcaga taaagccgca gcgtttatta aagagaaagg gcagtatccg atgaataaag
    19381 tcgcgaccga tgatatcggg tttgcgattt atcaggtgga tttgagtaag taaaacactt
    19441 ctttttcggc ctataaatca accgcatccg gcatttattg gcggatgcga tgctgccgta
    19501 tcttatccac cctacaagcc atgaaccgta gaccggataa gcgcagcgca tccggcagtt
    19561 atgccgcacg ttcatcccgc accgccaaca cctcgggcaa attcaactca atccagtccg
    19621 ccagtgcagc aaccttttcg ctcacctgct cgcccagcgg cgtgaggcta tattccacat
    19681 gcggcggcac caccgaatac gcgatacggt taagaaaacc atcctgttct aacgcctgta
    19741 acgactgcgc aagcatcttt tcactcaccc caccgatttt gcgccgcagg tcgctaaagc
    19801 gatgagtacc ttcgcgtagc gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt
    19861 tcaacacctc gcgcgacggg cactgttccg caaagagatt accctctttc agttgttgtg
    19921 acaggctaac ctgactcatt tcatacttac ctttttgtat gtacttacta aaagtaagtt
    19981 taggtgttag cgtatttaaa cacaagacaa aacgatggag acttcccatg atcgctatta
    20041 ctggtgccac tggccaactt ggtcactata ttattgaatc cttgatgaaa acggttcctg
    20101 ccagccaaat agtggctatc gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag
    20161 gcattaccgt gcgtcaggct gactacagcg atgaagccgc actgacatct gcacttcagg
    20221 gagtggaaaa actactgctg atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc
    20281 gtaatgttat taatgccgca aaggcggctg gcgtgaaatt tatcgcttat accagcctgc
    20341 tacatgcaga tacctccccg ctcggcctcg ccgatgagca catcgagacg gagaaaatgc
    20401 tggctgattc tggcatcgtt tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc
    20461 tcgccagcgc cccggcagca ctggaacacg gcgtatttat cggtgcggcg ggcgatggca
    20521 aaatcgcctc agcaacgcgg gcagattatg cgacagctgc ggcacgcgtg attagcgaag
    20581 ccggtcacga aggcaaggtt tacgaactgg cgggcgatag tgcctggacg ttgacacagt
    20641 tagcggcaga gctgaccaaa cagagcggca aacaggttac ctatcaaaat ctgagcgaag
    20701 ccgatttcgc cgcggcactg aaaagcgtcg gactgcccga cggactggcg gatatgctgg
    20761 cggattctga cgttggcgca tcgaaaggtg gtctgtttga taacagcaaa acgcttagca
    20821 aattgattgg ccgcccaacg acaacgttag ccgaaagcgt aagccatctt tttaatgtta
    20881 ataactagtt aattaaagtg gcatcctccc gcatcctctc tgataatgac gggatgccgg
    20941 gagcaatcat gtctgcttcc tgaacgtttc ttctgacaga ccaatggatg ccagtaatga
    21001 ttagcggcgt gctgtacgcc ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc
    21061 cgttgatcgt gccggaatac ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag
    21121 ggttaattgc gctacccatt gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc
    21181 gggactggct taccgccttg atgctcacta tgatgggcaa cctcatttat tacttctgcc
    21241 ttgccagtgc cattcaacgt actggcgcgc ctgtttccac gatgattatc ggcaccctgc
    21301 cggtggtgat tcctgtcttt gccaatctgc tttatagcca gcgcgacggc aaactcgcgt
    21361 ggggaaaact cgccccggca ctgatttgta ttggcatcgg cctggcgagt gtgaatattg
    21421 ctgagttaaa ccacggactc cccgattttg actgtgcacg ttatacctct ggcatcgtgc
    21481 tagcgttagt ttccgtggtc tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc
    21541 gggaaaatcc cgacaaacat ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc
    21601 cagtttctct catcggctat ctcgtcgcct gttactggct gaatatacaa acgccggact
    21661 tctccttacc ttttggcccc cgtccg
//
LOCUS       DADLSB010000056        21395 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.056, whole genome shotgun sequence.
ACCESSION   DADLSB010000056 DADLSB010000000
VERSION     DADLSB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.056"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            59..931
                     /locus_tag="JN331_002067"
     CDS             59..931
                     /locus_tag="JN331_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY7260216.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(991..2289)
                     /gene="eno"
                     /locus_tag="JN331_002068"
     CDS             complement(991..2289)
                     /gene="eno"
                     /locus_tag="JN331_002068"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY7260217.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2377..4014)
                     /gene="pyrG"
                     /locus_tag="JN331_002069"
     CDS             complement(2377..4014)
                     /gene="pyrG"
                     /locus_tag="JN331_002069"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY7260218.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4242..5033)
                     /gene="mazG"
                     /locus_tag="JN331_002070"
                     /pseudo
     CDS             complement(4242..5033)
                     /gene="mazG"
                     /locus_tag="JN331_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
     gene            complement(5129..7363)
                     /locus_tag="JN331_002071"
     CDS             complement(5129..7363)
                     /locus_tag="JN331_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAY7260219.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7411..8712)
                     /gene="rlmD"
                     /locus_tag="JN331_002072"
     CDS             complement(7411..8712)
                     /gene="rlmD"
                     /locus_tag="JN331_002072"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY7260220.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLGALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLQEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8769..11525
                     /gene="barA"
                     /locus_tag="JN331_002073"
     CDS             8769..11525
                     /gene="barA"
                     /locus_tag="JN331_002073"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY7260221.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11757..13097)
                     /gene="gudD"
                     /locus_tag="JN331_002074"
     CDS             complement(11757..13097)
                     /gene="gudD"
                     /locus_tag="JN331_002074"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7260222.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13118..14458)
                     /locus_tag="JN331_002075"
     CDS             complement(13118..14458)
                     /locus_tag="JN331_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7260223.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14460..15812)
                     /locus_tag="JN331_002076"
     CDS             complement(14460..15812)
                     /locus_tag="JN331_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7260224.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKSVAGQ
                     "
     gene            complement(16246..16695)
                     /locus_tag="JN331_002077"
     CDS             complement(16246..16695)
                     /locus_tag="JN331_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY7260225.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16713..17495)
                     /gene="truC"
                     /locus_tag="JN331_002078"
     CDS             complement(16713..17495)
                     /gene="truC"
                     /locus_tag="JN331_002078"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY7260226.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17495..17824)
                     /locus_tag="JN331_002079"
     CDS             complement(17495..17824)
                     /locus_tag="JN331_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY7260227.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18446..18991)
                     /gene="syd"
                     /locus_tag="JN331_002080"
     CDS             complement(18446..18991)
                     /gene="syd"
                     /locus_tag="JN331_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY7260228.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19059..19907
                     /gene="queF"
                     /locus_tag="JN331_002081"
     CDS             19059..19907
                     /gene="queF"
                     /locus_tag="JN331_002081"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY7260229.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20019..21383
                     /gene="ppnN"
                     /locus_tag="JN331_002082"
     CDS             20019..21383
                     /gene="ppnN"
                     /locus_tag="JN331_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY7260230.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5257 a         5701 c         5104 g         5333 t
ORIGIN      
        1 tctaaaatta ccgatgccgc cgtcaattta tttatccaga tttaatgaga aatttataat
       61 gcgctatttc atactaatgt tcactttcgt atgttccttt gttgcagccc aaccaactat
      121 tgtcccacaa ttacagcaac aagttacggg tcttacgagt agcttaaact cacaagaaaa
      181 gaaagaactg acgcacaagt tagaatctat tttcaataat acccaagtgc aaattgctgt
      241 attaattgtt tccacaacca aagatgagac aattgaacaa tatgctacaa gagtttttga
      301 caattggcgt ttaggagatg ccaaacgtaa tgatgggata ctgatcgttg ttgcctggtc
      361 ggatcgcact gtccgcatcc aggtaggtta tgggctggaa gaaaaggtaa ccgatgctct
      421 ggctggagat atcatccgta gcaacatgat acccgcattt aaacaacaaa aattagctaa
      481 gggattagag ttagctataa tcgctttgaa taaccaactc acttcacaac accaatatcc
      541 ggctaatcct tcagaaagtg aatcaacgtc ttccactgat cattattact ttgctatttt
      601 ttgggtattt gcagtgatgt tcttcccttt ctggtttttt catcaaggca gtaatttttg
      661 tcgcgcatgt aaaagtggag tctgtatttc agcaatccat cttttagatt tattcctgtt
      721 ctccgataaa actttttcca ttgctgtatt ttccttcttt tttactttca ccatatttat
      781 ggtctttacc tgtttatgtg tacttcagaa aagagcatct ggtagaagtt atcattcaga
      841 caacagcggt tccgctggag gctcaagtgg cggcggattt agcggcgggg gcggttcttc
      901 tggcggtggc ggcgcatccg gccgctggta actccaccat aaaaaatgcc agcccgaagg
      961 ctggcattat caaatcagat aaagtcagac ttatgcctgg cctttgatct ctttacgacc
     1021 gttgtacggt gctttttcgc ccagagcttc ttcgatacga atcagctggt tgtatttagc
     1081 aacacggtca gaacggctca tagaaccagt tttgatctgg cctgcagcag taccaacagc
     1141 cagatcagcg atggtagcat cttcagtttc accagaacgg tgagagataa ctgcagtgta
     1201 gccagcatct ttcgccatct tgattgcagc cagagtttcg gtcagagaac cgatctggtt
     1261 gaatttgatc aggatggagt tagcgatacc tttttcgata ccttctttca ggatcttggt
     1321 gttggttacg aacaggtcgt caccaaccag ctggattttg tcgcccagaa ctttggtctg
     1381 gtatgcgaaa ccgtcccagt cagattcgtc cagaccgtct tcgatagaaa cgatcgggta
     1441 ctgtttggtc agttcttcca ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc
     1501 ttcgccagcc agaacatatt taccatcttt gtagaattca gaagctgcgc agtccatcgc
     1561 cagagtgatg tctttgccca gttcataacc agcagcttta acagcttcag cgataacagc
     1621 cagagcttca gcgttggaac ccaggttcgg cgcatagcca ccttcgtcac caacagcagt
     1681 gttcatgcct ttcgctttca gaacttttgc caggtgatgg aaaacttcag aacccatgcg
     1741 gatggcttct ttcacagttt tcgcgccaac cggctgaatc atgaattcct ggatatcaac
     1801 gttgttgtca gcgtgctcac caccgttgat gatgttcatc atcggaaccg gcatggagta
     1861 tttgcccgga gtgccgttca gttcagcgat gtgctcgtac agcggcatac ctttagcagc
     1921 tgcagcagct ttggcgttag ccagagatac agccaggatt gcgttcgcgc cgaatttgga
     1981 tttgttttcg gtgccgtcca ggtcgatcat gatcttgtca atgccagcct gatctttagc
     2041 atctttgcca atcagcgcct gagcgatcgg gccgtttacc gcagcaacag ctttggttac
     2101 gcctttaccc aggaaacggg atttgtcgcc atcgcgcagt tccagagctt cacgggaacc
     2161 agtagaagca cctgacggag cagctgccat accgacgaaa ccaccctcca gatgtacttc
     2221 ggcttcaaca gtcgggttac cacgggagtc gatgatttca cgaccgatga tttttacgat
     2281 tttggacatt aggttttcct caagtcacta gttaaactga aactccagac aaacaacgcg
     2341 tacccagggt acgcgttgcc gttctaactt ttttacttac ttcgcctgac gtttctggaa
     2401 ctcgctggcg gctttcacaa agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt
     2461 aaactccgga tggaactggc aagccacgaa ccacgggtga ttcggaactt cgatgatctc
     2521 gaccaactga tcatccccgg aacggcccgc aacgcgcaga cctgcatctt caatctgttt
     2581 caacagcatg ttgttgactt cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt
     2641 gtatagctgg cgaaccaggc tatcgtcaac caactggcac tgctgtgcgc cgagacgcat
     2701 ggtaccgccg agatcgctct tctcgctacg aacttcaacg ttgccgtttt catcgcgcca
     2761 ctcggtaatc agcgccacaa ccgggtactt acagtctggc acaaattctg tagagttggc
     2821 gttctccatg ttggcaacat ggcgagcgta atcaattaac gccacctgca tacccaggca
     2881 aatgcccaga taaggaatat tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc
     2941 tacgccacga tagccgaaac cgccaggtac gaggattgcg tccagacctt taaggatttc
     3001 aacgccgcgc gtttcaacat cttgtgaatc gatcagtttg atgttgacgc tgacacgatt
     3061 cttcagccca ccgtgtttca gtgcttcgat cactgattta taagcatccg gcagttcaat
     3121 gtacttgccg accataccga tggtgacttc acttaccgga ttcgcttctt cgaagataac
     3181 ctgttcccat tcggacagat ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata
     3241 atcgtccagc ccctgagatt tcaacaggcc cggaatttta tagatggaat cgacgtcttt
     3301 cagagaaata accgcttttt ccggaacatt acagaacaat gcaatcttcg cacgttcgtt
     3361 cgccggtaca gcgcgatctg aacgacaaat caggatgtca ggctggatac cgatggagag
     3421 cagctctttt acagagtgct gagtcggttt ggttttgact tcaccagacg ctgccatgta
     3481 cggcaccagc gtcaggtgca taaacagagt gtgctcacgg ccaatttcaa cagccatctg
     3541 gcgaatagct tcgaggaacg gcaaggattc gatatcacct actgtaccgc cgatttctac
     3601 cagtactacg tcatgacctt cgccaccttc cagcacgcgc tctttgattg cgttagtgat
     3661 gtgcggaata acctgcacgg ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac
     3721 gtcagagtag atacgacccg tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa
     3781 acgctcgtag tgccccaggt ccaggtcggt ttcagcgccg tcttcagtaa cgaacacttc
     3841 cccgtgttgg attgggctca tagtacctgg atcgacgttg atgtaaggat ccagtttcat
     3901 gatggtcaca ttgaggccac gggcttcaag aatggctgcg agggaggctg cggcaatgcc
     3961 tttacccaga gaggatacga ccccgccggt cacaaaaata tagttcgttg tcatgctgaa
     4021 cctgagaagt taggttgaaa agacgatgga ataaccaaga cgggaaagca gtatacccga
     4081 acatgacctg tgccacaaac tttcattatc cctcctcttc gccagcgcac tattgaaatc
     4141 aggagtgaga aaatagcccc tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca
     4201 tttaaaaaat gacacaaatg gcgcttgacc gcgtaattcc cttagagatc aatttcctgc
     4261 cgttttacct gttgccagac ttcttccatt gtttcgaggt caacacctgt catttccagt
     4321 ccacgcgcgg caacaatacg ctccacttcg cgaaaacgac gctcgaattt ttcgttcgct
     4381 ttttgcaatg cgatttctgc tttcgtccct aaatggcgag ccagattaac cgtggcaaac
     4441 agcaggtccc ccatttcctc ctccagttta gcctggtcga caacagcctg ccgcgcctcg
     4501 tacatcacct cgtcgatctc ttcgtagact ttatcaacca ccgggccaag cgtcgtccag
     4561 tcgaagccaa cgttggcgca acgtttctgg attttttgcg cccgcattaa agccggtaaa
     4621 ctacgaggaa tatcgtccag cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg
     4681 atttgctccc aacgggcaag cacttcacta ctgttttcgg cagaactatc agcaaaaaca
     4741 tgcggatggc gacgctctaa tttatcgcta atagcagcgc aaatatcatt aaagtcaaag
     4801 cgcccttctt cctgagccat ttgcgcgtaa aacaccacct agaatagcag atcgcccagt
     4861 tcaccgcgaa gatcgtcaaa atcttcacgg gcgatggcgt ccagcacttc gtaggtttct
     4921 tcaagggtgt aaggcgcaat ggtggcaaat gtctgctctt tatcccacgg gcagccgttt
     4981 tccggatcgc gcaggcgctg cataatagtg agcaaacggt cgatttgatt cattgaattg
     5041 tcctgaaaat tgcgggtctg tcaggtgtaa taccttcgag taaaagatat cgctggatac
     5101 ggctgacgcc atatccagct atgtaagact aactcccgtg caaccgacgc gcgtcgataa
     5161 catccggcac ctggttgagt ttacccagca cgcgccccag cacttgcagg ttgtaaatct
     5221 caatggtcat gtcgatggtc gccagttgct gtttggtgtc gctacggctg gcaacgccaa
     5281 gcacgttcac cttctcgttg gcgagaatgg tcgtgatatc acgtaacaac ccactacgat
     5341 cattagccac cacgcggacc accagcgaat atccggcgga gtagctctca ccccataccg
     5401 cgtcaacaat gcgttctggc gcatgggagc gcagttccgc cagttgttcg caatcggcgc
     5461 ggtgtactga aataccgcgt ccctgggtaa tgaagccgac aatctcatct ccaggaatcg
     5521 gctggcagca gcgcgcgatg tggtgcatca ggttaccaac accttcaacc accacacgac
     5581 cgttatcttt actgcggttt tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg
     5641 cggcgtcctg ctcttcggca ctcggcttat taaattgcga ttgcaggaag ttcaccatct
     5701 gattgagacg gatatccccg ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt
     5761 tgtaacgcgg cagcagatgt ttttctgctt ctttcaggct gatccccaga tgttccagct
     5821 cgtcgtcaag gatttgccgc ccagccagaa tgtttttgtc acggtcctgt ttacggaacc
     5881 aggcgtgaat tttcgaacgc ccacggctgg ttgtgacgta accgaggttt gggtttaacc
     5941 agtcacggct ggggttcggc tgtttctggg tgataatttc aatctggtcg cccatttgca
     6001 gctggtaggt gaacggcaca atgcgcccgc caatttttgc cccgatgcag cggtgtccga
     6061 catcactgtg gatgtggtaa gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga
     6121 catcaccttt cggcgtaaag acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt
     6181 cgagcatttc gccggaatca gccatctctt cctgccacgc aatcagttta cgcagccagg
     6241 caatccggtc ttcatgtccc gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc
     6301 agtgcgcagc aacacccaac tccgcatctt catgcatctg tttggtgcgg atttggatct
     6361 caacggtttt tccacccggc cccagaacca cggtatgaat agactgataa ccgtttggtt
     6421 tcgggttagc gacgtaatcg tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta
     6481 tccccagtgc ggcataacaa tcctgtaaac gctcggcgac aatacgtacc gcacgcacat
     6541 caaacagctc atcgaaggcg aggttctttt tctgcatttt gcgccagatg ctgtagatgt
     6601 gtttcggacg accatacact tcagctttaa cgccttcagc tttcatctca gcgcgcagat
     6661 ggccgacaaa ctcttcgata tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg
     6721 cgatgcgttt gtattcggtc gggtggagat aacggaagca gtaatcttcc agctcccatt
     6781 tcagttgccc aatcccaaga cggttcgcca acggcgcgta gatattggtg cactcttttg
     6841 ccgccagtac acgttcatct tccggcgcat cttttacttc gcgcagatga gcaatacgct
     6901 ccgccagttt gatgactacg cagcgaaaat catcgaccat cgccaataac atccggcgaa
     6961 cgttatcgac ctgttcggag gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg
     7021 ccgccatatc acgcacgccg tgaataaggt taacaaccga cttaccgacg ctctcacgca
     7081 gcacatcttc gctgactacg ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg
     7141 tgtcaatgtc catacttaat gtcgagagga tctccaccat ctcaacacca cgccacaata
     7201 acagactggc atccggatgc ccctgcgtct gttgcagaca atacgcccag gtttcggcta
     7261 agcactcaca cgacttctgg ctggtaatac ccagacttgc gatccatttt tccggatcaa
     7321 attcaccagc cttattgata tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg
     7381 accagacctg ccgaaatcgg caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga
     7441 ttccagatgt cccgtgtgtg ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc
     7501 tgcttttaat aacgcttcgc tatcccgagc cagcgttgca gggttacagg atacataaac
     7561 tatacgaata ggttccagtt ttataatttg ctgcataaca cctgcggcac ctgctcgcgc
     7621 cgggtccagc aacactttat cgaagccgtt tttcgcccac ggctgctttg tgacatcttc
     7681 ttgaagattt tcgtgataaa acgtcacatt ctgtaagcca ttaagacgcg cattctgctg
     7741 gcctttttcc accagcgccg gaacaccttc tacaccgacc acactggcag cttgtgtcgc
     7801 caatggcagt gtaaagttgc ccataccgca gaacagatcc agtacgcgat cttcaggttg
     7861 cacatccagc cattccaacg cacgcgctac cattttttgg ttcacacccg cattgacctg
     7921 aataaaatcg cgcgggctaa aagttaagcg caacccgttt gagtcatacc agggcatctc
     7981 accagagacg gtttcgagta tctcactatc gggggcgaga tacagatcca ggccttcaga
     8041 atgcgaaaag cgttccagtt tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa
     8101 aatcatcagc gtgccgctgg ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc
     8161 ttgtaagctg cccagacatg ccctgacttt gggcagcaat gctccaagtt ggggcgctaa
     8221 aatggggcat tgtttgacgt cgacaatgtc actggagccc gctttgcgaa accccatctg
     8281 aagttgctgt gttttcggta agtagttcag acttaaacgc gcgcggcggc gatagcccca
     8341 gggaacatcg gcgatcactt cagagacatc gtgtttcatt aatcgggcga gtgccgcact
     8401 tttgcttcgc tgctgtaaat ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc
     8461 aaaatgagga cagcgtggcg tttcgcgttc cgggctatcg cttaaccggc gtacgacttt
     8521 agcgcgggca tactgttttt tatcttcagt aacagcaact tccgcgtttt cctgcggcaa
     8581 taatccgggg ataaataacg ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc
     8641 gaggtcgttg actgaaacgg ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta
     8701 gaattgcgcc attggcgaga ctttctcaat ttaacagtgt gaccttaatt gtcccataac
     8761 ggaactccat gaccaactac agcctgcgcg cacgcatgat gattctgatc ctggcaccga
     8821 ccgtccttat tggtttattg ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc
     8881 agcgtcaact ggaagatgcc ggtgctagca ttattgagcc gcttgcagtt tctactgaat
     8941 atggcatgag cctgcaaaat cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc
     9001 gccattccga tattgttcgc gcgatttcgg tttatgatga aaataaccga ctctttgtca
     9061 cctccaattt tcatcttgat ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc
     9121 gccagctcac tgtcactcgt gacggcgata ttatgatcct ccgcacgccg attatttctg
     9181 agagttactc ccccgacgaa tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg
     9241 gatatattgc gcttgagctg gatcttaaat cggttcgctt gcagcaatat aaagagatct
     9301 ttatttcctg cgtgatgatg ctgttttgta tcggtattgc gcttattttt ggctggcgct
     9361 taatgcgcga tgtaaccggt ccgattcgca acatggtgaa taccgtcgac cgcatccgtc
     9421 gcgggcaact cgacagccgg gtggaaggat ttatgctcgg cgagctggat atgctgaaaa
     9481 acggtatcaa ctcgatggca atgtcgctgg ctgcttatca cgaagagatg cagcacaata
     9541 tcgaccaagc gacgtccgat ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg
     9601 agttggatct ggcgaaaaag cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg
     9661 caaatatgtc acacgagctg cgtacaccac tgaatggtgt tattggcttt acccgcctga
     9721 cgctgaaaac agaattaaca ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg
     9781 caaataattt gctggcaatt attaatgatg ttctcgactt ctcgaaactg gaagcaggta
     9841 agctgattct ggaaagtatt ccattcccac tacgcagcac gctggatgaa gtcgttactc
     9901 tgctggcaca ttcttctcac gataaagggt tggaactgac gctcaatatt aaaagcgacg
     9961 tgcctgataa cgtgatcggc gacccactgc gattacagca aatcatcact aacctagtgg
    10021 ggaatgcaat taaattcacc gagaatggca acattgatat tctggtagaa aaacgtgcgc
    10081 tgagtaatac caaagtgcag attgaagtgc agattcggga taccggcatt ggtattcctg
    10141 aacgcgatca atcgcgctta ttccaggcct tccgacaggc tgatgccagt atttcccgcc
    10201 gtcatggtgg caccgggctg gggctggtga ttacacaaaa actggttaat gaaatgggcg
    10261 gcgatatttc gttccatagc cagccgaatc gcggttcaac tttctggttc cacattaatc
    10321 tcgatctgaa cccgaacatt attatcgaag ggccatccat ccagtgcctc gcaggtaagc
    10381 gcctggccta tgtcgaacca aactccgcag cagcgcaatg cacgctggat attttaagtg
    10441 aaacgccgct ggaagtggtt tatagcccaa cgttctccgc gctgcctccc gcgcattacg
    10501 acatgatgtt gttaggcatc gcggtgacct tccgcgagcc gctaacaatg caacatgagc
    10561 gattagcgaa agcggtatcg atgaccgatt tcctgatgct ggcacttcct tgccatgcac
    10621 aagtcaatgc tgaaaaactc aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa
    10681 cacctacgcg cctgttgcct gccctgacgg aattttgtca tcacaaacaa aacacgcttt
    10741 tgcctgtaac cgatgaaagt aagctggcaa tgacagtcat ggcggttgat gacaaccccg
    10801 ctaacctgaa acttatcggc gcattgctgg aagatatggt gcaacatgtg gaactttgcg
    10861 atagcgggca tcaggcagtt gaacgggcga aacagatgcc gttcgatttg atcttaatgg
    10921 atattcaaat gccggacatg gatggcattc gggcctgcga actcatccac caactcccgc
    10981 atcagcgaca aacgccggtt atcgcggtaa cggcgcatgc aatggccggg caaaaagaga
    11041 agctgcttgg cgcagggatg agcgattatc tggcgaaacc gattgaagaa gagcgattgc
    11101 ataatttgtt gttgcgctac aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg
    11161 aagtcaacga aattgtggtg aacccgaatg cgaccctcga ctggcaactg gcactacgcc
    11221 aggcagcagg aaaaaccgat ttagcgcgcg atatgctgca aatgttactc gatttcctgc
    11281 ctgaagttcg caacaaagtt gaggaacagc tggttggaga aaacccggaa ggcctggttg
    11341 atttgattca taaactgcat ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc
    11401 tctgccagct gatcgaacaa cagctacgta gcggtactaa agaagaagat ttggagccgg
    11461 agctgctgga actgttggac gagatggata atgtcgcgcg cgaagccagc aaaattctcg
    11521 ggtaacggac gtcttgtcga gtagcaatcg gctttttatg acgctggttt cagattggtg
    11581 acaaagtgcc tgtgtttatg ccggatgcgg cgttaacgcc ttatcctgcc tacaaaacca
    11641 attaaattca atgaattaaa aaatgatgta gacctgatac atagcacaac aggctatttt
    11701 gcgtttagca tcagtctcaa accggctcca gatagagccg gttttggttt tctgtcttaa
    11761 cgcaccatgc acgggcgctt gttatcgaac gtccagccag gaatcagata ctgcattccc
    11821 atcgcatcgt cacgcgcgcc aagcccgtgt ttctgataca gctcatgggc tttcatcact
    11881 tgatccatat cgatttctac acccagcccc ggtttttctg gcacctgtac cagcccgcct
    11941 ttgatctcaa acggttcttt ggtcaggcgc tgattacctt cctgccagat ccagtgcgta
    12001 tcaatagcag taattttacc cggtgcagcg gcggcaacat gggtaaacat cgccagggaa
    12061 atatcgaagt ggttgtttga gtgtgaaccc caggtcaggc caaattcatg gcacatttgc
    12121 gccacacgta ccgaaccttg catagtccag aaatgcggat ccgccagcgg gatatcaacg
    12181 gattgtaacg agagcgtatg gcccatctgc cgccagtcgg tggcgatcat attggttgca
    12241 gtcggcaggc cagtcgcgcg acggaactca gccatcactt cacgcccgga gaaaccttgc
    12301 tccgcaccac acggatcttc tgcataagcc agcgaacctt tcaggtattt accaatttta
    12361 attgcttcgt tcagcgacca ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag
    12421 cgttgcgcca gtgccacaat agactcggct tcttcttccc cggccagtac accgcctttc
    12481 agtttgaaat cgttgaagcc atatttttca tacgccgctt ccgccaggcg caccaccgca
    12541 tccggcgtca tcgcttcttc atgacgcagg cgataccagt cgcatgagtc atccggctgg
    12601 ctttgatacg gcagaggtgt ggctttgcga ttaccgacga agaacagata accgagcatt
    12661 tcgacttcgc tacgctgttg accatcgccc agcagcgatg ccacgtttac ccccagatgc
    12721 tgccccagca gatccagcat tgccgcttct atcccggtaa ctacatgaat agtggtacgc
    12781 aaatcaaatg tctgcaaacc gcgcccacta gcatcacgat cggcaaaagt attacgcacc
    12841 agcgtcagaa cgtttttgta ttcacccagc gttttaccta ccaccagcgg aatcgcatct
    12901 tccagcgttt tacggatttt ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa
    12961 ttatctttga taatcacaat attacgcgtg aagaacggtg cgtgtgcacc actcagattc
    13021 atcagcatac tgtcatgacc cgccaccggg atgacctgca tttcagtaac aacaggcgtc
    13081 gtaaattgag aactcatatt tatgtccttt ttcagaatca atgacggccg aaaacgggac
    13141 gtttacggtc aaaggtccag ccggggatca ggtactgcat cggacctgcg tcgttacgcg
    13201 caccgcaagg cagacgttta taggcctcat gtgccttttg tacctgttcc cagtccagtt
    13261 ccacgcccag accgggcgca tcaggaacgg caatttttcc gtttttaatc tccagcggat
    13321 ttttggtcag gcgacaatcg ccctcctgcc aaatccagtg ggtatcgata gcggtaggat
    13381 tacccggtgc cgccgcgccc acatgggtaa acatcgccag agagatatcg aaatggttgt
    13441 tggaatggca gccccaggtc agcccccagt cgtcgcaaag ctgcgccacg cggactgcac
    13501 cggaaagcgt ccagaagtgc ggatcggcaa gtggaatatc taccgcattg agcatcaccg
    13561 catgacccat ttcgcgccag ttggtggcga tcatgttagt cgcgacgggc aagccggtcg
    13621 cccgtcgaaa ctccgccatc acttcacgtc cggagaagcc ctgttctgcg ccgcatggat
    13681 cttcggcata ggtaagaaca tcattcagcc ctttgcataa agaaatggct tcatcaagca
    13741 gccatgcacc gttgggatca acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac
    13801 gaacagtgtc gatttcttgc tcgccaggta acaccccgcc cttaagtttg aaatctttaa
    13861 agccatagcg atcctgtgag gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt
    13921 tctgatggcg caactgatac cactcatggt tgcccggcgt attttccaga taaggaagat
    13981 cggttttggt ccgatcaccg atataaaaca gataaccgag gacggtaata gtctcgcgtt
    14041 gcttgcctgg ccccaacagt tcgcagaccg gaacattcag cgccttacct agcaggtcaa
    14101 gcaaggcggc ttccagcgcc gccacggcat taacgcgcaa ttcaaaagtc caggcacctt
    14161 tgccgaaggt atcaaaatcg gctgcctgat tacctttatg cacctgctga accactttat
    14221 tcaggcgcgc aacttcctgg cccagaacca tcggaatagc atcgaccagt gtttgataaa
    14281 tcacctctcc gcccggcgct tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca
    14341 cgatattgcg ggtgaagtat gcgttatgcg caccaccaat attgagcaac atgctgtcat
    14401 gtccggcgac cggaatgact ttcatatcag taataacagg actggattgt gtcgccatct
    14461 tattgccccg cgacagattt cagctcgata cgcttgatat cgcccaccag caccaggtag
    14521 ctcagtaccg cgattagggc atgaacacca acataaatca gcgccccatt aaacgagcca
    14581 gtcgtgccaa cgatataacc aattgcgatt ggcgtgacga tgccagaaat gttaccgaac
    14641 atgttgaaca ggccaccgga aagaccgctg atctcttttg gcgcggtatc tgccattact
    14701 gcccaaccca gcgccccgat gcccttaccg aagaaggcca gcgccataaa gccgatgatc
    14761 atccactcaa cgttgacgta gttgcagaac accatcacca tcgacagcaa catgcccatt
    14821 acgatcggtg ttttacgcgc aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa
    14881 ataattccac ccagcacacc gccgataaaa ccgcaaaccg ccggaacgga agccacaaag
    14941 cccgctttca gaatcgacat cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg
    15001 aaaaagtaag tcagggcgtt gatacagtac tgaccgatat aaacgccgat catcatccgt
    15061 gacccgagca gctgtttgat ctgcccccac ttcacgctga acggcacttt aactttggtg
    15121 ttttgctgat ccatattgat cagcgcgcca cccgcggcga tatactccag ctctttctga
    15181 tttacccccg gatgttgatt tggctcgtgg atgactttca accagataaa gctgatgaca
    15241 atgcccagac cgcccataaa gaagaagacg tgtgaccagc ccacttcatg cgtcagccag
    15301 cccataatcg gcgcgaagat caccgttgcg aagtattgag cggagttaaa aatcgacacc
    15361 gccgttcccc tttcctgcgc cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa
    15421 gatggcgctt cagcaagccc gaccaggaag cgcagagtaa acagggcaac gataatgccg
    15481 aatccactaa agatatcgac gaagccttgc agcaaggtaa acatcgacca gataaagatc
    15541 gaccagaagt agacgcgttt tgaaccaaaa cgatccagca accaaccacc agggatctgc
    15601 ccgataacat aagcccatga gaaggcagag aacacatagc ccattcccac gggatcaagg
    15661 ccgatatctt tggccatttc cgaaccggca atagaaagcg tagctcggtc gccgtagttg
    15721 aaggatgtga cgataaacaa catcaccact atccagtaac gagcattcgt gcgtttttcc
    15781 acactgctcg cagcctgact taaagaactc attgttgcac tcctgaaaat tcgcgttagc
    15841 cacgctcact ctggactgcg acatcgccag gaaatcagag gtgacgtagg gtgttttttg
    15901 ccgtttttat aggtcgttcg ccgaatacgg cgcgtgttta tatctggcaa tagcagtata
    15961 aaaagcgcgc catagcggct caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc
    16021 cgattttggc attagcccta gacggtggaa tccacttcac ggaaatgaaa acaagaacaa
    16081 gaaaggaagg gtaaaaacga agaaataaag aagagtatga aatggatcgc ttgactctag
    16141 gcaaacgcca gtaaaaatcc gcgctatgaa gcagttttta ctggcatttg cctgaaaaga
    16201 ttcgattcag caccgctaaa acgacattta ccgctcgctg acatatcagg acaacagcgt
    16261 gccccactgt tcgacccacg gatttgattc cgtttccggt tccgggttct cactggcgtc
    16321 aatcaacagc atttcgccaa cccgctgagc gctctgttcc tgcaacaagg catcgaactg
    16381 tttgccgcca ttgcaaaaat ttacataact gctgtcgccg agcgcaatca cgccataacg
    16441 cagattcggc tggaagccca gactatcttt gattccctga aagagcggca caatgctatc
    16501 aggaaggtcg ccctgcccgg tcgtggacgt aaccaccaga acatacttat cctgataggg
    16561 cagccagtcg cttaattcag gatcttcaaa taccgttgct ttgtggccct gtgcggtcag
    16621 aattgcttct gcttcttccg ccactaacag cgaatttccg tacatggtgc cgacaaaaat
    16681 accaatttcc gccataccgt tttctccctg gattaggaac ttatctctcc atcctgaccc
    16741 gatggcgcac tgaactcaac cctttcattt tcaggaagca gaccgcgcca gccaaattgt
    16801 gataacgcct gcatccaggt gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta
    16861 aaaggatgcg tcaatgacaa ctgactggca tgtagcatta accgctggag gccaaaatgc
    16921 tcagcaccgc tgcgattctg gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga
    16981 cgcaaatggg caagatgtcg gcgaagctga tgtttgcgtc cggttttcgg ctccagttcc
    17041 accaggccgt agcgcgtggt cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc
    17101 agaccgcgat aatgcgtcac tgctggctgc gggcctttat cttcgcgggc aaatttatca
    17161 gcgattttgt ccagttcttc caccagtgga taatccaaca ccgcttcttc catcaaccag
    17221 ccgcgcacaa tcgcatggta acgtttctgg atttggtgct gttcaaactg ttgtgccagc
    17281 agccgtccgg cctcgctgga taatcccatc aacaacacac cagaagtggg tcggtccaga
    17341 cgatgagcag taaaaacatg ctgtcctatc tggtcacgca cggtttgcat gaccactact
    17401 ttctcgtcgc gatccagcca gctacggtga accagccagc cggagggttt atttaccgca
    17461 accagccatt catcctgata gagtatttcc agcattagct cgcatcatcc gcaaaaagag
    17521 catccagttt ttccaactca gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg
    17581 ccatttcata atagggtgca acagcaaaag cgcccggtaa cggctggttg ttatttaaca
    17641 aatcgtgcat tcgcgggatc agcccccact gcaaccactc cagtggttcc atggtgtcca
    17701 taaagaacgg ttgggtacta ttaaattgat gcggctgggg ttcatcgttt cgccagtgct
    17761 gatgttcacg cagtaacgct tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg
    17821 tcataaagca acctcaataa gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga
    17881 gcactgtatt cacagtgctc ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc
    17941 ctgctcatcc ttgacaactt ttcctctggc cttgcggcca atcgttcatc ctgaactatt
    18001 gcttcctgct cacaccaccc cgatgtgata cttcatcctg aagtgtccct ggccttcctg
    18061 acccaccgaa tcatcctgac cggttctcat tctccatcct ggaggtgtcc tttaacgcgt
    18121 cctgcgtcat cctcttcgct tcatccagaa gcctttccct gaaacaccat cctggtgtgt
    18181 cctgcagaag tgtcatcatc ctgatgttca cttcgttgtc tgactccctg tcgacgaaga
    18241 tagaatcgtc tttttcgcga agtcttacaa ggcgcttaca gacaatgcct taaggaaaat
    18301 ttcatatgaa agttaagaat taacaatcaa cattttgatt ttaaatgaaa ataacgattc
    18361 ttatcttttt aagtattcgc ttatttcctg gcgatctcct acagagtctg taagagatct
    18421 ctcacaacca gatggggtat ttggattaca gaagaggctt aagttgatta aggaattccg
    18481 caagatttga ggctaaatgc gtccgtttac gtgttcccag cgtctctttg cacacttcac
    18541 cggagagatt acacaccgag atgacctcta actcttcttc cagcgtcgcg ataaatagcg
    18601 tgggcggcag ttttagcctt ttctgagtca ccagatgacc aatcagattt tcctgcaccc
    18661 gacggaagtc gtcttcactc caggtttgca gcaatgtcag tttgatatcg ccaaactgcg
    18721 cgtgcatatc cccggcaaac tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa
    18781 tatcaaaagc gcgttcaacc gcgtttacat tttgttcccc ggtaaacggc tggggttgcc
    18841 agtatacggc atcttcggta gttgaaataa tgcacggtga aggaacaccg tacagttcct
    18901 cacttaacgg ccagctttta tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat
    18961 ctttcagggc ttgtgcggtc aaatcgtcca ctaatttctc tcttcactta aaccagatac
    19021 acttgtcgtt tagtttatct ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc
    19081 aggcacttgc gggcctgact cttggaaaat caaccgatta ccgggatacc tatgacgcca
    19141 gcctactgca aggcgttcca cgcagcctga atcgcgaccc gctgggtctg aaagcggata
    19201 acctgccttt tcacggtacg gatatctgga cgctgtatga actttcctgg ctgaatgcga
    19261 aaggtttgcc gcaggtcgct gtcggtcatg ttgaacttga ttacaccagc gtaaatctga
    19321 ttgagtcgaa gagttttaag ctctatctca acagttttaa ccagacgcgt tttaataact
    19381 gggatgaggt gcgccagacg ctggagcgcg acttaagcac ttgcgctcag ggtaagatta
    19441 gcgtggcgtt atatcgtctt gatgaactgg aaggccagcc gataggtcat ttcaatggca
    19501 cttgcattga tgaccaggat atcactatcg ataactatga attcactact gactatctgg
    19561 agaatgccac cagcggtgaa aaagtagtgg aagagacgct cgtcagccac ctgctgaaat
    19621 caaactgcct gatcacccat caaccagatt ggggttcgat ccaaattcag tatcgtggac
    19681 gccaaattga cagagaaaaa ctgctgcgtt acctggtctc attccgtcat cacaacgagt
    19741 tccacgaaca gtgcgtggaa cgcatcttta atgacctgtt acgcttctgc cagccagaga
    19801 aattgagcgt ttacgcacgt tatacccgtc gtggcggtct ggacattaac ccgtggcgca
    19861 gtaatagcga ttttgtccca tcgaccacaa gactggttcg gcaataaatt ttttctcaat
    19921 tttgcgtgct ggattcacgc agaaggttgt gaaaggtcat caggcagggc tattgtaatc
    19981 aaagggaatg acgatattcg tcccataagg agtttttctt gattacacat attagcccgc
    20041 ttggctccat ggatatgttg tcgcagctgg aagtggatat gcttaaacgc accgccagca
    20101 gcgacctcta tcaactgttt cgcaactgtt cacttgccgt actgaactcc ggtagtttga
    20161 ccgataacag caaagaattg ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc
    20221 gtgaacgcgg cgtaaagctg gaactgatta atcccccgga agaggctttt gtcgatgggc
    20281 gaattattcg cgctttgcag gccaacttgt tcgcggttct gcgagacatt ctcttcgttt
    20341 acgggcaaat ccataatacc gttcgttttc ccaacctgaa tctcgacaac tccgtccaca
    20401 tcactaacct ggtcttttcc atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc
    20461 caaatatggt ggtctgctgg ggcggtcact caattaacga aaacgagtat ttgtatgccc
    20521 gtcgcgtcgg gaaccagctg ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag
    20581 gagcgatgga agcgccgatg aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag
    20641 acagtcgttt tattggtatg acagagccgt cgattatcgc cgctgaaccg cctaacccgc
    20701 tggtcaacga attgatcatc atgccggata tcgaaaaacg tctggaagcg tttgtccgta
    20761 tcgctcacgg catcattatc ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt
    20821 tgctgggaat tttaatgaac ccggccaaca aagatcaggt tttaccattg atcctcaccg
    20881 gcccgaaaga gagcgccgac tacttccgcg tactggacga gtttgtcgta catacgctgg
    20941 gcgaaaacgc gcgccgccat taccgcatca tcattgatga cgccgctgaa gtcgctcgtc
    21001 agatgaaaaa atcgatgccg ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca
    21061 gctttaactg gtcaatgcgc attgcgccag atttgcaaat gccgtttgag ccgtctcacg
    21121 agaatatggc taatctgaag ctttacccgg atcaacctgt tgaagtgctg gctgccgacc
    21181 tgcgccgtgc gttctccggt attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca
    21241 ttgaagagtt tggtccttac aaaatcaacg gcgataaaga gattatgcgt cgtatggacg
    21301 acctgctaca gggttttgtt gcccagcatc gtatgaagtt gccaggctca gcctacatcc
    21361 cttgctacga aatctgcacg taatctcccc tcccc
//
LOCUS       DADLSB010000057        21264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.057, whole genome shotgun sequence.
ACCESSION   DADLSB010000057 DADLSB010000000
VERSION     DADLSB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.057"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            14..922
                     /locus_tag="JN331_002083"
     CDS             14..922
                     /locus_tag="JN331_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260231.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1161..1220
                     /locus_tag="JN331_002084"
     CDS             1161..1220
                     /locus_tag="JN331_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7260232.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1537..1596
                     /gene="ibsE"
                     /locus_tag="JN331_002085"
     CDS             1537..1596
                     /gene="ibsE"
                     /locus_tag="JN331_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY7260233.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1716..3149)
                     /gene="hldE"
                     /locus_tag="JN331_002086"
     CDS             complement(1716..3149)
                     /gene="hldE"
                     /locus_tag="JN331_002086"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY7260234.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3197..6037)
                     /gene="glnE"
                     /locus_tag="JN331_002087"
     CDS             complement(3197..6037)
                     /gene="glnE"
                     /locus_tag="JN331_002087"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY7260235.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDVAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SRLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVSARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6060..7361)
                     /locus_tag="JN331_002088"
     CDS             complement(6060..7361)
                     /locus_tag="JN331_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY7260236.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVMAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7603..8223
                     /locus_tag="JN331_002089"
     CDS             7603..8223
                     /locus_tag="JN331_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY7260237.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8287..9525
                     /gene="cca"
                     /locus_tag="JN331_002090"
     CDS             8287..9525
                     /gene="cca"
                     /locus_tag="JN331_002090"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY7260238.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9688..10509)
                     /gene="bacA"
                     /locus_tag="JN331_002091"
     CDS             complement(9688..10509)
                     /gene="bacA"
                     /locus_tag="JN331_002091"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7260239.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10600..10968)
                     /gene="folB"
                     /locus_tag="JN331_002092"
     CDS             complement(10600..10968)
                     /gene="folB"
                     /locus_tag="JN331_002092"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY7260240.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11073..11690
                     /gene="plsY"
                     /locus_tag="JN331_002093"
     CDS             11073..11690
                     /gene="plsY"
                     /locus_tag="JN331_002093"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY7260241.1"
                     /translation="MSAIALGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11703..12635)
                     /gene="ttdR"
                     /locus_tag="JN331_002094"
                     /gene_synonym="dan"
     CDS             complement(11703..12635)
                     /gene="ttdR"
                     /locus_tag="JN331_002094"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HAY7260242.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            12842..13685
                     /gene="ttdA"
                     /locus_tag="JN331_002095"
                     /pseudo
     CDS             12842..13685
                     /gene="ttdA"
                     /locus_tag="JN331_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13682..14287
                     /gene="ttdB"
                     /locus_tag="JN331_002096"
     CDS             13682..14287
                     /gene="ttdB"
                     /locus_tag="JN331_002096"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY7260243.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14335..15798
                     /locus_tag="JN331_002097"
     CDS             14335..15798
                     /locus_tag="JN331_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY7260244.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLLIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JN331_002098"
     CDS             complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JN331_002098"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY7260245.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17092..17307
                     /gene="rpsU"
                     /locus_tag="JN331_002099"
     CDS             17092..17307
                     /gene="rpsU"
                     /locus_tag="JN331_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY7260246.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17418..19163
                     /gene="dnaG"
                     /locus_tag="JN331_002100"
     CDS             17418..19163
                     /gene="dnaG"
                     /locus_tag="JN331_002100"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7260247.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19358..21199
                     /gene="rpoD"
                     /locus_tag="JN331_002101"
     CDS             19358..21199
                     /gene="rpoD"
                     /locus_tag="JN331_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY7260248.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5118 a         5881 c         5465 g         4800 t
ORIGIN      
        1 acaagaagcg tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca
       61 gaatgcacgt attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat
      121 tctggctgaa cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag
      181 aaaggttgag gctgaacgtg aagttcgtat taaagagatc gaacagcagc aggtcaccga
      241 aatcgccaac cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc
      301 agaagcgcgt gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac
      361 cactcgccag accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca
      421 ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc
      481 agagatgcag gcggcggcta tcgttgagtt agccgaagct acacgtaaaa agggtctggc
      541 ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac
      601 cagccttaaa ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt
      661 tgagccgatg aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg
      721 cagcgctgcg ggtgatgcaa acacgggtaa tgtggggggc ggaaacctag cggaacaagc
      781 attatcagcc gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga
      841 aattggcgtt tcaggcggtt cactggcggc attgacttca cccttaacct caacaactcc
      901 cgtcgccgaa aacgtagaat aaattttcag ctatgaacgt ccctagcaca aaaagccggg
      961 gattttttat atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg
     1021 cgtgaccgct cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct
     1081 catcaacaat cggggagtca gcaaagggct gaaacgggaa agcccctccc gaggaagggg
     1141 ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttactcg
     1201 taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt
     1261 tcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct
     1321 ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg
     1381 acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg
     1441 cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc
     1501 cctcccgagg aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg
     1561 gttgtgttgt tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat
     1621 cctcccttac ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc
     1681 ctgctacgaa gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc
     1741 ttcttgatga tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg
     1801 ccgttggccc agacttcttt acttccggcg atctcttctg gtttatagtc gccccccttc
     1861 accagtaaat ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac
     1921 accacccagt cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta
     1981 acggggcggg aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc
     2041 aagcggtcac ccagtttgcg ggcatttgcc agataggaga cgtgtccggc gtgcaggatg
     2101 tcaaagacgc cattggtcat caccactttt tcaccgcgtt tacgcgctgc cgctacggcc
     2161 agcttcagtt cctcttcggt catcacgcca aagccggtat ctgcgcgccc acgcactgca
     2221 ttttccagct cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc
     2281 gccgcattgg caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc
     2341 aggacgccaa tcaccgtgtc gcctgcaccg gtaacgtcat acacttcctg cgcctgggtt
     2401 ggcatatgca gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc
     2461 actaacagag ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct
     2521 tcttcggtct tacatttacc gacaacagct tcaaattccg agagattcgg tgtcagcagc
     2581 gtagcgccgc ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc
     2641 gctttacgcg ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca
     2701 gaaagcacca gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc
     2761 ggatcaacac cttcgaaacc ttcttcaaaa tccagacgga tcagctgttg gttgcgggaa
     2821 agtacccgta atttggtgat ggtcggatgc gtcggtacag aaacgaagtc gcatttgacg
     2881 ttgacgtcgg ccagagattt actcagcgcg cgcgctgcat cgtcaatgcc cgtcaacccg
     2941 accaggcgtg cattagcacc gagagaagcg atattcatcg ccacgttagc cgcgccgccc
     3001 ggacgttctt cgatggtatt cactttaacc acgggcaccg gcgcttccgg cgagatacga
     3061 ctggtggggc cgtaccagta acgatccagc atcacatcac caaccaccat cactcctgca
     3121 cgttcaaact ctggcagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc
     3181 gcgataatac catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagat
     3241 cacgctctgc ggtgaagcaa tcctccgaca catggcccgg caattcctgt aatgccagat
     3301 gatgaagttc atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct tcctgctctt
     3361 ccataatgtc gttttgcgcc agcagctcca gaatacgcac gttgtctgac cagcgcgtta
     3421 acttcggttt ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg
     3481 taattccgcc ttcatcggct ttgatatcaa agcgattacg atgtttattg ccgagatgag
     3541 cgcgcatttt ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tctcgcggca
     3601 gtgtcataat ctcacgacgc accgcgtcaa agtgcgcagt gagctgcgga tcgccgtaca
     3661 ctacacgcgc actcaccagc gcctgatgtt cccacgtcca ggcctcgttt ttctgataat
     3721 cggcaaatgc ttctgcggat gtcaccagca ttcccgccgc cccggacgga cgcagtcgag
     3781 catccacttc atacaaaatg ccggaagagg tacgcgtact gaacagatgc ataatgcgtt
     3841 gcgccagacg cagataaaac tgccgcccgt cgatttcccg ctcgccgtca gtcatcgcat
     3901 ccattgggca atcatggagg aagataaggt caagatcgga actgtagcct aactcccagc
     3961 cgcccagctt gccgtagccg accaccgcaa aaccgcgccc ttcacgttcg ttgaggtgat
     4021 ttggcttacc gtagcgggcc accatttgac cccacgcctg ctgaacgacg gcatctatca
     4081 tagcttctgc cagccaggtt aagtgatcgc tcactttcat taccggtagc gtaccggcga
     4141 tatccgctgc cgcaatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct
     4201 gttgttcttc gtcatcttcc ggcacgcgca gcaaatactg gcgcagttca tcacggtagg
     4261 catcggtcgc cgtcggctgg taaagggtat ttggatcgag caattcatcc agcaataatg
     4321 gataacgcgc tagccggctg gcaatcatcg gcgatgcggc acacagagaa atcaaatgtt
     4381 taagcgccgc agggaattca ctgagcaatt ctaaataggt tgtgcgggta acaatcccca
     4441 ccagcaaggc ggtaatgcgc gacagcgtga cagcaacgtc ttcacgcgca cagacatcac
     4501 ttagcagatg tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atagtacgct
     4561 tatccagctc tttgcggaaa tcagcaatca gcgttaggac ctgtttgcga tcatcctcgc
     4621 taagatgcgc cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac
     4681 gccactgttc cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat
     4741 taaacacccg gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt
     4801 cagcaaagtc catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgttt
     4861 gggtttgttc gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatatg
     4921 ccactcgcaa ttgttcagca tcgttttcag aaagcagatg cagcgcggca atggcgctga
     4981 gcgttggcag taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga
     5041 acacctgaac gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg
     5101 tcaaaccacg tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa
     5161 tcacgctgaa atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt
     5221 tagcatagac gccttcgcta tcgcccataa tccgcgcttt gaccatcgcg taacgctccc
     5281 agtcgcgtcc ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc
     5341 cactttcgcc aaacggacgc agtcgcatat ccacgcgata gacgaagcca tcctgcgttg
     5401 gttgatccag cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca
     5461 gttcccggcg tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat
     5521 cagaggagaa attcagttcc ccgccgccca gcttacccat gcctaagatc agcagcggtt
     5581 gcgcttcgcc ctgcgcattg cacggcgttc cccactcgcg gcagcaggcg tcatacagcc
     5641 agtcacgcgc cgcaacaatc agcgtctccg ccagataact gagctgctgc aatatgctct
     5701 cttcagtaac cagtgccagc gtttgcgccc atgcgatgcg caccataatg cgccgccgga
     5761 atagccgcag ctcgcgcatt aacccggctt cgtcgctcac attactgagc gcctcctgca
     5821 accacacgct gtaatgttgc cattcgtcag cctgtggcgg ttggctttcc agttccgtca
     5881 gccactctgg atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact
     5941 tcgcctgtgc actaagtgat tcctcggcta aaagctctgg cagccgctca acaacggtct
     6001 gccagtactg ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat
     6061 taacgttttc cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt
     6121 tcaatttcaa tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc
     6181 caggcttgcg cgacgacagg atcatagtaa cccgccagca acagtattga gtcaatatca
     6241 cgcatcaggc gtggcaactg gtcacggtag cgatcgccta acggctggca gaaaacgctt
     6301 ttcagttcag cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggagtcgcta
     6361 attttgccct gcgctttggc atctaaaaac ggctgccatg ctttgcttac cagccattcg
     6421 gtcaacgcca gcttcgccat tgccgtttcg gtagagtaga ccgccatcac ggcagaaacc
     6481 gcagaagcga tggtcgcttc gcattgagtc agcaaatcac gtaagtgagt gctcgcttta
     6541 cgcggcacaa tgccaccgaa cagcatcagt gtatgacgga ctaggctaat ggctgccagc
     6601 acctgctctt tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc
     6661 gctaacacca gctcgagcgc cgcttccagc ccctgttcca catcggcttt tgccgcaaca
     6721 tgcaaaatgg tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca
     6781 cgcgccgctt tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc
     6841 agtttcagca ccgcgcgcgt gtcgccgcta agcagctcca gttccagctc gcagataggc
     6901 tcagcaaatt cacccgcttt cacttccccc tggtcgagag cgatttcaat tcggctgcca
     6961 tcgatctcta ctagccattt ttcgcgataa aaatcggtgc taaacagcgg ctgcacgcgg
     7021 gaagcgagat cggcaggcaa ttcgccgttc ggccagattt ccgtcggtaa ctgcgccagg
     7081 tcgagcgtcg gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc
     7141 actcttcctg caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag
     7201 cccatatcgt gcctacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc
     7261 acggggtcat ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca
     7321 ctgtgattaa caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta
     7381 tgtcacataa gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta
     7441 ttttgcgcca aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg
     7501 caagacctga cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc
     7561 cactactatc gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg
     7621 atcggattaa ctttactcgc acttagcgcg actgccgtct cacacgctga agaaacgcgc
     7681 tatgtttctg acgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc
     7741 gtgggcacgg ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat
     7801 tatgcccagg tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc
     7861 actgagccaa gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc
     7921 gataaactca ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa
     7981 gtggcgcaga gcgacagcgt gatcaacggg ttaaaagaag aaaatcagaa actgaaaaac
     8041 gagctgattg tcgcgcagaa aaaggtcgat gccgccagcg tacagctgga tgacaaacag
     8101 cgcaccatca tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg
     8161 ctcggtctgg tactgccgca cctgatccca agccgcaaac gcaaagatcg ctggatgaac
     8221 taaatcgcct tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat
     8281 gatggcgtga agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctatcg
     8341 gtcaaagaca gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc
     8401 taccagcagg taggccgcga ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat
     8461 gcgctggcac gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc
     8521 gcaccggatg tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg
     8581 gcccaggacg ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat
     8641 cgtctgttgc gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt actgcgcgtg
     8701 gcgcgttttg ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg
     8761 ctgatgcgcg agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg
     8821 aaagagacgg aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc
     8881 gattgcggcg cacttcgcgt tttattcccg gaaattgacg cactgtttgg cgttccggca
     8941 cctgccaggt ggcatccgga aatcgacacg ggtattcata ccttaatgac gctctcaatg
     9001 gcggcgatgc tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt
     9061 aaaggactga cgccgccaga actttggccg cgtcatcatg ggcatggccc ggcgggtgtt
     9121 aagttagtgg aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc
     9181 agactggtgg ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaacc
     9241 atcgtcaaat tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg
     9301 gcgctgacca gcgaggctga cgtgcgcggc agaaccggtt ttgaatcagc ggactacccg
     9361 caaggccgtt ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc
     9421 gttgaagcgg gatttaaagg cgtggagatt cgcgaggagc tgacccgacg gcggattgcg
     9481 gcggtagcca gctggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg
     9541 ttagcacgaa ccgtcccctc gccccttcgg ggagagggtt agggtgaggg gaacagacca
     9601 gcaccggtgc gaacaatcac ccccaccccg gccctctccc tacagagcga gggagaaaac
     9661 ccggcctgct cccgaacctt ttctcaatca aaagaacacg acatacaccg cagccgccac
     9721 aataaagcga taaatggcga acgggataaa cgaaatgcgc ttaatcaatt gcaggaaggt
     9781 tttaatcgct atcagcgcca ccacaaaagc ggtgataaac ccaacggcaa acatcgggat
     9841 atcgccggtt gtcaggaagc cccagctttt gtagagatcg agcgccgttg cgcccatcat
     9901 catcggtacc gccagcagga acgagaactc ggaagcagcg taacggctca cgcccatcag
     9961 catcccacct gaaatggtcg ccccggaacg ggagaaaccc ggccacagcg ccagacactg
    10021 gaaacagcca atcataaacg cctgacgata ggtcatatca tcaagacccg gcgcacgcgg
    10081 ctctttcggc ttcaggcatt cggcggcaat cagcaacaaa ccgccaacga ccagcgcata
    10141 catcacattt atcgggttaa acaatgactt aatcgtgtcg tggaataata gccccagtac
    10201 caccgccgga atcatcccca gcaaaatgtg gatcagcgtt aaacgacctt tgctttcacc
    10261 ttcgtgctgc aacgggcggc caaagtggat gccaatcagg ccaaacagac gccgccagaa
    10321 catcactact accgccagaa ttgatcctaa ctggatcaca acttcaaagg ttttcgccgt
    10381 gtcgccctca aaccccaaca agtgaccgac aataatcata tggcccgtgc tggataccgg
    10441 cagaaattct gtcaatcctt cgaccacacc caatattgcc gctatcagca gcgagtgcat
    10501 atcgctcatc aataaacccc taaattatta aaatgtaccg cttgtccgaa ctactgcagt
    10561 atgaccaggt tataaccgtt tggtttaaca gctgtaaaat taattatttt ctttcagatt
    10621 attgccacgc tcaatgatta cgccaacatt cgccgcccgc gccactgcgc ctggcttgct
    10681 gagtttgata cgcacccacg gcgagttgaa gcgtgctaac agcagctccg ccacctcttc
    10741 agccacgcgt tccaccagcg caaaacgcgc cccctcgacg tggctgacca ccgtttctgc
    10801 aatgtcagcg taactgaggc aatccgccac atcatcactt ttcgccgctt tacggttatc
    10861 ccatgccatt tcgatatcga acactaactt ctgttcgatg gtctgttccc agtcgtaaac
    10921 accaatagtg gtgattaccg aaagttgctc tataaataca atatccatca cgtcctgcct
    10981 gctttttggc taaccggata ccacttccgg cgaaatgtgc gtattatcca cagattcatc
    11041 gttgaacacg aattttcaaa acggaacagc ttatgagtgc aatcgcgctt ggaatgatcc
    11101 tcatcgcgta cctctgcggc tccatttcca gtgccattct ggtttgccgc ttgtgtgggc
    11161 tgcccgatcc gcgaaccagc ggctccggca atccaggcgc aaccaatgtg ttacgtatcg
    11221 gtggcaaggg agcagccgta gcagtactga ttttcgacgt tctgaaagga atgttgcctg
    11281 tctggggcgc gtatgaatta ggtgtcagcc ccttctggct aggcttaatt gccatcgccg
    11341 cctgtcttgg acacatctgg cccgttttct tcggttttaa aggaggaaaa ggcgttgcta
    11401 ccgcttttgg agcaatcgca ccaattggct gggatctcac cggagtaatg gcgggaacct
    11461 ggttactgac cgtgctattg agcggatact cgtcgctggg agcgattgtc agtgcactga
    11521 ttgctccgtt ttatgtctgg tggtttaagc cacaattcac cttcccggtt tcgatgctct
    11581 cttgcctgat cctgctgcgt catcatgaca acatccagcg tctgtggcgt cgtcaggaga
    11641 caaaaatctg gacgaaattc aaaagaaagc gcgaaaagga tcccgagtga tttctggtgg
    11701 atctacatga cctgatagcc ttcatcgggc ttgcccagcc gttgctggca ccatgccgcc
    11761 agaaattcca cgcagacacg taatttcatg ctgcgataga gcggctcccg gtaaacagcc
    11821 cagatattgg cgctttgtgc atactccggc aatacttgca ccagtttgcc actctccaga
    11881 aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag cgcccattgc
    11941 aacactatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga ttttttctcc
    12001 tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt caccagacag
    12061 tcatgacgac ttaattcctg taatgattgt ggctgcggat atttttgcag atactcaggt
    12121 gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata atcaggaatt
    12181 tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat ttgccgatcg
    12241 aacaattcaa aatgcacctg taactcagga taattgcgca taagttcggt aatggctggc
    12301 gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatccc ctccgggcgc
    12361 gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat ttccagcgca
    12421 tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga gcggttcagc
    12481 agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtt
    12541 tgcccaagcg tcgctgctgc agcactaaaa ctcccgctgt ggacgatttc taccagtacc
    12601 tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca gttgtgataa
    12661 gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt cattcttatt
    12721 ttcgcgttga ttcacgaact gttaattaca tattcacggt ggcaaaaaat ataaaaccac
    12781 atttttagtg gtagtttgtg gcggtgaatt tttccagaca aatacaaaaa ctggagttgc
    12841 catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacagaga ttgtcgctaa
    12901 ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac taaaacagct
    12961 aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt tcgacaacat
    13021 gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga ttatgttctt
    13081 cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac tcaaacaagc
    13141 cgtggaatag gcaaccgtca aagcgccact acgtcacaat gcggtagaaa tttttgacga
    13201 agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg acatcatccc
    13261 cgacaatgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca cgctacctgg
    13321 ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtggtgaaat tcgtcttcga
    13381 aaatatctcc accctcgccg taaacgcctg tccaccggta ctggtgggcg tgggcatcgc
    13441 cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc attttgcgcc cgattggcag
    13501 ccgccatccc aatccaaaag cggcagaact ggagctacgc ctggaagaag gactcaaccg
    13561 tctggggatt ggtccacaag ggctgaccgg caacagttca gtgatgggcg catcgtcgcg
    13621 gcacgctgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt
    13681 tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcgg
    13741 cgatgtgatc tacctgaccg gtacgctggt gacctgccgc gacgtttgtc accgccgttt
    13801 gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg
    13861 ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc tccgtcggcc cgacaaccag
    13921 tatgcgtatg gaaagttttg aacgtgaatt tattgagcag accggcgtaa aactggtggt
    13981 tggcaaaggt ggtatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca
    14041 tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtagaag agattgaaga
    14101 agtgcactgg acagaactcg ggatgccgga gtcactgtgg gtctgccggg tcaaagagtt
    14161 cggcccgctg attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa
    14221 gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat
    14281 caaataaccc tcccggagag gctcacccct ctcctttttc gcaggcataa cacgatgaaa
    14341 ccttccactg aatggtggcg atatcttgcg ccgctggcgg tcatcgccat tattgctcta
    14401 cttccggttc ccgcagggct ggagaaccat acctggctct actttgccgt ttttactggc
    14461 gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat ggtgggtatc
    14521 tccatcatcg ccatactctc tccctggctg ctgttcagcc ccgaacagct cgcccagcca
    14581 ggctttaagt tcaccgccaa atccctctcc tgggcagttt ctggtttttc taattcggtt
    14641 atctggctca ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac cgggcttgga
    14701 cgccgcattg cgctgattct ggtgaaaaag atggggcatc gcacgctgtt tctcggctat
    14761 gcggtgatgt tctccgagct gatcctggca cctgtaacac cgtccaactc ggcgcgtggt
    14821 gcgggtatta tctatcccat catccgtaac ctgccaccgc tctatcaatc acaaccaaac
    14881 gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt tgccgactgc
    14941 gtgaccagcg ccattttcct gacggcgatg gcacctaact tgctgttaat tggactgatg
    15001 aaaagcgcat ctcacgccac gctgagttgg ggcgactggt tcctcgggat gttgccgctc
    15061 agcattttac tggttctgct ggttccctgg ctggcttacg tgctgtaccc gccggtactg
    15121 aagtctggtg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat gggcccgctc
    15181 tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct gtggattttc
    15241 ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcgct gatgctgctt
    15301 ctgctcatta tcagttggga cgacattgtc agtaataaag cggcatggaa cgttttcttc
    15361 tggctggcct cgcttatcac cctcgcgacc ggactcaaca ataccggttt tattagctgg
    15421 tttggcaaac tgttagcagg cagcttaagt ggttattcgc cgacgatggt gatggtgacg
    15481 ttgattgtgg tgttttatct actgcgctac tttttcgcca gcgccacggc gtatacctcc
    15541 gccctcgcac cgatgatgat cgccgccgcg ctggcgatgc cggaaatccc gctgccggtg
    15601 ttctgcctga tggttggcgc ggcaattggt ctggggagca ttcttacacc atacgccacc
    15661 ggacccagtc cgatttacta cggtagtggt tatctgccaa cggcggatta ctggcgactg
    15721 ggggcgattt ttgggctgat attcctcgtg ttgctggtga ttaccggctt actgtggatg
    15781 cccgtggtgt tgctttaagt gagggcggtg ccggaaattg ctctccggca cccaatagtt
    15841 ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc acgctaacgc cgagatccgc
    15901 cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc cgttatcagt
    15961 acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt tttttcatca tttcagccag
    16021 cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc atgaccagtc gcttaaagcc
    16081 cgtctgatcc aacgctcgct tacacttaat catcaacgta tcgaccaccg catcttcaaa
    16141 ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg ttgtcacgaa tcgtgttcgc
    16201 cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg tcatcggacg
    16261 cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta acggtccgcc
    16321 aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccgg cggcatcatc
    16381 gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta actgcgtatg
    16441 accgccggac accagcagcg cgacaaacgg aaattccggc gggttatctt ccagcatcgg
    16501 cgctaacaga tgcccttcca tatggtgtac cgggattgcc ggaacgttcc aggcaaacgc
    16561 cagagaacgc cccacggttg cgccaaccag cagtgcgccg actaatccag ggcctgcggt
    16621 ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg ccgcctggat
    16681 caacggtacg gttttacgca catgatcgcg ggaggccagt tcaggcacga cgccgccgta
    16741 gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt tttcatcgtc
    16801 gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac gcatgacttg
    16861 ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt
    16921 tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa
    16981 ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata
    17041 agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta
    17101 attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc
    17161 gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc
    17221 gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa
    17281 aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga
    17341 gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt
    17401 gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg
    17461 cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga
    17521 atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg
    17581 agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga
    17641 tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga gctggcagca atgcacaatc
    17701 ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa
    17761 cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg
    17821 ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc
    17881 gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca
    17941 atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac
    18001 gcagttacga tcgtttccgc gaacgggtga tgttccccat tcgcgataaa cgcggtcggg
    18061 tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg
    18121 aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata
    18181 acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc
    18241 aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac
    18301 aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc
    18361 gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc
    18421 tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta
    18481 aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt ctgtttaaca
    18541 gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg
    18601 cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat
    18661 taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg
    18721 cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta
    18781 tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg
    18841 atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc
    18901 agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca
    18961 cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct
    19021 tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa
    19081 tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa
    19141 accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc
    19201 cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt
    19261 ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc
    19321 aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca
    19381 gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa
    19441 tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat
    19501 caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc
    19561 tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga
    19621 atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt
    19681 tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa
    19741 ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta
    19801 cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc
    19861 gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga
    19921 agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga
    19981 tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta
    20041 cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga
    20101 gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta
    20161 cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat
    20221 gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg
    20281 caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga
    20341 aaaactgcac gatgtctctg aagaagtgca tcgcgccctg caaaaactgc agcagattga
    20401 agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg
    20461 tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat
    20521 ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg
    20581 taacatcggc ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc
    20641 cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg
    20701 caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg
    20761 ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct
    20821 gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga
    20881 aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct
    20941 cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct
    21001 ggctggcctg accgcgcgtg aagcaaaagt tctgcgtatg cgtttcggta tcgatatgaa
    21061 caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg
    21121 tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg
    21181 tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag
    21241 tctctctgca caaacgccac cttt
//
LOCUS       DADLSB010000058        21035 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.058, whole genome shotgun sequence.
ACCESSION   DADLSB010000058 DADLSB010000000
VERSION     DADLSB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.058"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..584)
                     /locus_tag="JN331_002102"
     CDS             complement(<1..584)
                     /locus_tag="JN331_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7260249.1"
                     /translation="MNTTNGYNLALQRQLVDSKINDISDLKQKLEFSKIGSASDGMSV
                     TSTVEECEKHGKYTSYEKYLTISGKRITSSKSECPQCLEEKIRKKEIECEQAEQRARQ
                     SKIEYLLNSLNIPERFKNCTLQNYEPVNDDAKRVLKVCQAYASKWPERLQKGGGLVMC
                     GKPGTGKNHLALAIARHAIIEHQSSVIFTTALKIA"
     gene            complement(587..1453)
                     /locus_tag="JN331_002103"
     CDS             complement(587..1453)
                     /locus_tag="JN331_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260250.1"
                     /translation="MARSRNIKPGFFTNDELAECSPYARLLFAGLWTIADKEGRLDDR
                     PKKIKAMVLPFDDVDCNDLLQQLHSHKFINRYQVNGGSYIQINNWKKHQNPHCKEAAS
                     EIPEPVENNNSTMQVQCKDDKDEEKEDVESLQFIENNEAPEKHHASTVQEPVENSLNP
                     ADSLNLIPDSLSNTQAADATCEGGGADVHKISSRYAFEGNIVRLNHKDYEAWKRLYPN
                     IDLNYELERLDIEFSHEKPKNWFITASQKLSYQNKQALSRPVRKVANGLKAENFAAKD
                     YGQTEMPSWAQE"
     gene            complement(1465..2265)
                     /locus_tag="JN331_002104"
     CDS             complement(1465..2265)
                     /locus_tag="JN331_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7260251.1"
                     /translation="MAKKPTPDQIKKIRSGITKKIRFEVFKRDGFKCQYCGSSAPDVI
                     LHVDHINPVSKGGDNDMMNLITSCDSCNGGKSDKLLSDNSIMEKQRQQLQELNTKREQ
                     LEMMIKWRDGLKSLKDDVVDIVATKIDDCIAPFTVNDNGRKSIKRWLRIYKLEEILDA
                     IEIAADKKLTQEITHELTGEFFEYIPRIAATKRKPPKEQRILYIRGILKNRIYINQNH
                     VMSYLKAWLSYDLDLDELTEFAKTVPNWTTFKEWVSERIREAQKELPY"
     gene            complement(2287..2586)
                     /locus_tag="JN331_002105"
     CDS             complement(2287..2586)
                     /locus_tag="JN331_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260252.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSEQIQMEF"
     gene            complement(2725..2949)
                     /locus_tag="JN331_002106"
     CDS             complement(2725..2949)
                     /locus_tag="JN331_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7260253.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGQVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            3094..3768
                     /locus_tag="JN331_002107"
     CDS             3094..3768
                     /locus_tag="JN331_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7260254.1"
                     /translation="MRSAGVSQSQLADMVGISQPAIQKMSSGKTNGSRKMVELANALK
                     VRPEWLSSGIGEMRDGTHEEPSNIRESSLKAVVWEDIKKNDDEFVALPLLNVSLSAGS
                     GSCEIEESSEFSLVFRKYYLKKMGVTESSAKLVRVTGQSMEPTLHDGDVVGVNTQDTT
                     IRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEFHKTVRIIGRVF
                     WSSHSW"
     gene            3806..4147
                     /locus_tag="JN331_002108"
     CDS             3806..4147
                     /locus_tag="JN331_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HAY7260255.1"
                     /translation="MAFTDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRADPDCCFFG"
     gene            4679..4870
                     /locus_tag="JN331_002109"
     CDS             4679..4870
                     /locus_tag="JN331_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260256.1"
                     /translation="MAPKKKAVADVQVGVKTFELPIPLRGDLIVTIGNLPRDLTKDEA
                     KRISLIVESFAMIDGGTKE"
     gene            5675..6058
                     /locus_tag="JN331_002110"
     CDS             5675..6058
                     /locus_tag="JN331_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7260257.1"
                     /translation="MTRRTQFKGNSRSRRRERLKAKALANGVLTREEAISSEVLHRPT
                     LSRAQIQAKGTHETPERIEDAKPIKFMAQDVIWQQKEYRRNLERAAIVYANEFGHKQP
                     ETGVCLPNVALYAAGYRKSKQLTAR"
     gene            6120..6743
                     /locus_tag="JN331_002111"
     CDS             6120..6743
                     /locus_tag="JN331_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260258.1"
                     /translation="MSSIRLTTRIKEVIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEVDQLSSRLMAISKELKKLGCSFYSCDVLSTSIYLAVSGRSVGWYSYG
                     KDSNGEDIWLPTPTRDKCMFDAGHEITKRFDEICALQQKLEARKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASTALPALRVEDLNKMIGLPSEAA"
     gene            6849..6989
                     /locus_tag="JN331_002112"
     CDS             6849..6989
                     /locus_tag="JN331_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HAY7260259.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDVLNQPGVPK
                     NG"
     gene            6982..7095
                     /locus_tag="JN331_002113"
     CDS             6982..7095
                     /locus_tag="JN331_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HAY7260260.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVEAYKKWRSK"
     gene            7092..7280
                     /locus_tag="JN331_002114"
     CDS             7092..7280
                     /locus_tag="JN331_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260261.1"
                     /translation="MIPVELAKTPELSRLKREYHIAEARYWRKAGDKSKKQLCLWQAQ
                     RERMNEREFLSSPSELPF"
     gene            7289..7945
                     /locus_tag="JN331_002115"
     CDS             7289..7945
                     /locus_tag="JN331_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7260262.1"
                     /translation="MGTATLILGESGTGKSTSLRNLPPEKTLIIQCINKPLPFPSSDW
                     VKISKENNNGNIYRTDNTENIVRALINAPHEIVVIDDYQAVMVNELMGKCNERGYDKF
                     TSIGKNAWDVFRAAGEGADTRRVYILAHTQTDDFGYTRMKTVGKLVDSTLVPEGYFTI
                     VLRTHVQNGKYSFSTQTNGSDCCKSPVGMFDSQFIDNDLYEVDKKICSYYGFEKNETS
                     "
     gene            7932..8537
                     /locus_tag="JN331_002116"
     CDS             7932..8537
                     /locus_tag="JN331_002116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260263.1"
                     /translation="MRRLEVSGERFGNLVVIGDAGMRGTQRLVNCVCDCGVVCVKQLG
                     NLRSGHTRSCGCSRSSTTKARKNKHGMYGTPTYKSWSSMLTRCFNERNSKYKHYGGRG
                     ITVCERWLDFKLFFADMGKRPDGTTLGRIDNNGNYEPSNCEWQKSIEQARNKSNTVLF
                     KFNGVLATISEHCEKTGLNPSTVRSRIYTYHWSVDKALSKG"
     gene            8542..9129
                     /locus_tag="JN331_002117"
     CDS             8542..9129
                     /locus_tag="JN331_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAY7260264.1"
                     /translation="MSNVIFTYNEEAALTAGQGGFINETGAHIITITEAELKQSEKGA
                     KFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGANVVHAMMGCVGIKQLTQHMVSASK
                     FVAPEFHGKKIGLVLQKVLTTNKKTGADSYQMEIRIPFIAQTGQTLKEKSEGKQPETI
                     ANMVASLKDKDNRAKNVSQNHADDYGYSQNDYPPF"
     gene            9153..9449
                     /locus_tag="JN331_002118"
     CDS             9153..9449
                     /locus_tag="JN331_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HAY7260265.1"
                     /translation="MPAPLYGADDPRNCSGSSKAEVLENIKNNFDAFLALTPETKAER
                     MYRRDIQLALKQEKDRTNETAMRPLRKATIDKFPEYIDPRLRNYRSRYGAISND"
     gene            9460..9624
                     /locus_tag="JN331_002119"
     CDS             9460..9624
                     /locus_tag="JN331_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY7260266.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFGSVNDNKY
                     LNAMWRSGKK"
     gene            9645..9974
                     /locus_tag="JN331_002120"
     CDS             9645..9974
                     /locus_tag="JN331_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001476141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HAY7260267.1"
                     /translation="MDGFLKGKCIPRDLKVNETNAEYLVRKFDEVRAEARNEGINYTA
                     SRLAAAFNHGFINKSLREVFDVTRMILSAKEELANEQHPIDGLSGGYAEKSLEEWAEQ
                     IRKGGNQ"
     gene            9971..10474
                     /locus_tag="JN331_002121"
     CDS             9971..10474
                     /locus_tag="JN331_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7260268.1"
                     /translation="MSKIDYQALREAAEQATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNLQYIKSRDQEN
                     EEIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAQTVT
                     VKEVGDA"
     gene            10547..11161
                     /locus_tag="JN331_002122"
                     /pseudo
     CDS             10547..11161
                     /locus_tag="JN331_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550159.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            11286..12068
                     /locus_tag="JN331_002123"
     CDS             11286..12068
                     /locus_tag="JN331_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000993708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7260269.1"
                     /translation="MNTQISVETLSPITYKQTNVITSELLAQLYGTDVVRIRQNHNEN
                     KSRFVEGVHFYKVVGDELRALKHKVALNYFVKIAPNARHLILWTERGAARHAKMLETD
                     QAWDVFEKLEDGYFNQREKVANHPQPVPQPESSLDRARAMEIALNVADRLVNMFPNLS
                     SESKQAAVAGVFNPIVGRDVVPLPAISEHYYSATEVGQRYGISANKVGRIANTYMLKT
                     EKYGKWFIDKSAHNDKQVETFRYNEAGVHKIEELIEGGRKAA"
     gene            12394..13050
                     /locus_tag="JN331_002124"
     CDS             12394..13050
                     /locus_tag="JN331_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7260270.1"
                     /translation="MATQISVESLSPITYNHIPVITTELLARLYGTETNNIKVNYTRN
                     AGRFVCGKHYFKLEGGELREFKNKVTHSNLVAPRAKHLILWTERGAARHAKMLETDQA
                     WEVFEKLEDCYFSPGKAEQHPQIQQQFTDEEIILLCYMQVQMENALDICKRLYPILKE
                     LNSSYTSKLYDIAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF
                     "
     gene            13135..13353
                     /locus_tag="JN331_002125"
     CDS             13135..13353
                     /locus_tag="JN331_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7260271.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            13331..14401
                     /locus_tag="JN331_002126"
     CDS             13331..14401
                     /locus_tag="JN331_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7260272.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            14536..15819
                     /locus_tag="JN331_002127"
     CDS             14536..15819
                     /locus_tag="JN331_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY7260273.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(15960..18140)
                     /locus_tag="JN331_002128"
     CDS             complement(15960..18140)
                     /locus_tag="JN331_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY7260274.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(18323..19756)
                     /locus_tag="JN331_002129"
                     /pseudo
     CDS             complement(18323..19756)
                     /locus_tag="JN331_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
     gene            complement(19832..20884)
                     /locus_tag="JN331_002130"
     CDS             complement(19832..20884)
                     /locus_tag="JN331_002130"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY7260275.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT         6029 a         4801 c         4930 g         5275 t
ORIGIN      
        1 gcaattttca acgcagtggt aaatatcaca gagctttgat gctcgataat ggcgtgccta
       61 gcgatagcca gtgcaagatg gttctttcca gttccaggct taccacacat gaccagtccg
      121 ccaccttttt gaaggcgttc aggccactta ctggcatatg cctggcatac cttaagaact
      181 cgctttgcat catcgttaac aggctcataa ttctgaagag tgcaattttt gaacctctct
      241 ggaatattga gagaattcaa caagtattca atttttgatt gtcttgctct ttgttctgcc
      301 tgctcacatt caatctcttt cttacgaatt ttctcctcaa ggcactgtgg gcactcagat
      361 ttacttgaag taattctttt tcctgagatg gtcagatatt tctcatagga agtatattta
      421 ccgtgtttct cgcactcttc cactgtgctg gtaactgaca ttccatctga tgctgatcca
      481 attttgctaa actcaagttt ctgtttcaga tcagaaatgt cattgatttt tgagtctaca
      541 agttgccttt gcagtgccag attgtaacca tttgtcgtat tcatattcac tcctgcgccc
      601 atgaaggcat ttcagtttgt ccgtaatctt tggcggcaaa gttttcagcc ttaagtccat
      661 tagcaacctt gcgtaccggt ctggataacg cctgcttgtt ctggtagctg agtttctggc
      721 tggcagtgat aaaccagttc tttggcttct catggctaaa ttcgatatcc agcctttcca
      781 gttcgtaatt caggtcaatg ttcggataca ggcgcttcca tgcttcgtag tccttgtggt
      841 ttaaccgaac gatatttcct tcgaaggcat agcgacttga aattttatgg acatcagccc
      901 cacccccttc acaagtcgcg tcagcggctt gggtgttaga aagggaatca ggaatcaggt
      961 taagggaatc agcaggattt aaactgttct ctactggttc ttgcactgta cttgcatggt
     1021 gcttttctgg tgcttcattg ttttcaatga attgcagtga ttctacatcc tccttctctt
     1081 catctttatc gtccttgcac tgtacttgca tggtgctgtt attgttctct actggttctg
     1141 gtatctcact ggcagcttct ttgcagtgag ggttctggtg ctttttccag ttattgattt
     1201 ggatatatga gccgccattt acctgatatc ggttgatgaa tttgtgactg tgaagttgct
     1261 gaagcagatc attgcagtcc acatcatcga aaggcagtac cattgcttta attttctttg
     1321 ggcgatcatc caaccgccct tctttgtcag caatggtcca taacccggca aaaaggagac
     1381 gggcatatgg agaacactca gctaattcat cattggtgaa aaagcctggt ttgatatttc
     1441 ttgaacgtgc cattttgtcc acctttagta cgggagttct ttctgagctt cacgaatgcg
     1501 ttcagatacc cactccttga acgttgtcca gttaggtact gttttcgcaa actcagtaag
     1561 ttcatcgaga tctaagtcgt atgaaagcca tgccttgaga taactcatta catggttttg
     1621 attgatatat atgcggtttt taaggattcc tctgatgtat agaatccttt gttcttttgg
     1681 cggttttctt tttgtcgctg ctatgcgagg gatgtactca aagaattcac cagtaagctc
     1741 atgggttatt tcttgggtga gttttttgtc tgcggcaatc tcaatcgcat cgaggatttc
     1801 ttcaagctta tatattcgaa gccatctctt gatagatttt cgcccattgt cattaacagt
     1861 aaatggcgca atgcagtcgt caattttggt agcgacaata tcaacaacat cgtctttcag
     1921 gctcttaaga ccatctcgcc acttgatcat catctccagt tgctctcgct tggtattaag
     1981 ttcctggagt tgctgccttt gcttttccat tattgagtta tcgctgagca gtttgtcgct
     2041 tttcccgcca ttacaactat cgcaagaagt aataaggttc atcatgtcgt tatcgccacc
     2101 cttgctaact gggttgatat ggtcgacatg aaggatgacg tctggcgcag aactcccaca
     2161 gtactggcat ttgaagccat cgcgcttgaa aacctcaaac cgtattttct tggtaatgcc
     2221 ggaacgaatc ttttttatct ggtctggtgt aggttttttt gccataatta actccgtaga
     2281 aagcagttaa aattccattt gtatttgttc agaacgctcg gtcttgcaca ccgggcgttt
     2341 tttattggtg agtccatcaa gcgcatactt aaaagccctg ctaatcggac tgatgtctga
     2401 tgccattccg aaagcacaca agaccgaagc aataaaccgc cagtcggtac gactgatctt
     2461 cgattcatga cagccaatca tctttgccag accgcgctgg gtaagcgttg acaggttgat
     2521 gagtaaatct gtttctgcac gatcaacgtc gcgctgtgat agtttgctgt aacttgtttg
     2581 ttccatttct taagatttcc aatagtgaat agttagttga aagctatgcg tggaaacgca
     2641 tatggcctta gttggtcaga tatcttgggg ctcgctttgt cagcgacgta ggacgaatgc
     2701 ccattgtgaa aatagcggtg ttacttatgc tgcctgattc ggttttggaa acaggtgtgg
     2761 taaatctggg cgaatttcgt aagccttgat ctgccctcca gtggcgttaa cgatggcggt
     2821 aactttctct ggagaaacct gcccgccttt cagccatttg tgtactgctg gctgcgttac
     2881 accacacttg tcggcaagcc gcttttgact accgacaatc ttcaaggctc gttgaattac
     2941 taaattcatg agcatacctc tttcagccat tacttataac caaggataac ccaagttata
     3001 aaagatagca ataaccttta ttattttact ttggataacc gtagttatag atttgtggat
     3061 atgaaaacat tcgcagaaag actaaatgca gccatgagat cggcaggggt gtcgcaatca
     3121 cagcttgcgg acatggttgg aatatctcag ccagccatac agaagatgtc atccggtaaa
     3181 acaaacggat ctcgcaagat ggttgagtta gctaatgctt taaaagtacg ccctgaatgg
     3241 cttagttctg gtattggtga aatgagggat ggtacacatg aagaaccatc caatatccgt
     3301 gagtcatctt taaaagctgt ggtatgggaa gacattaaaa aaaacgatga cgagtttgtc
     3361 gcattgcctc ttcttaacgt ttcgctttcg gctggaagcg gtagctgcga gatagaagaa
     3421 tcatcagagt tctctttggt tttcagaaag tactatctga aaaagatggg ggtaactgaa
     3481 agttctgcca agctggtaag ggtaacaggt caaagcatgg aaccaactct tcacgatggc
     3541 gatgttgttg gtgttaacac acaagatacc accatcagag atggtaaaac ctacgctatt
     3601 tgccagtctg atttgttgcg agtaaaaaca ttaatcgcca ctcctacatc ggtgataatc
     3661 agatcaataa atcgcgaaga gtacccggat gaagtaatgg atagagatga atttcataaa
     3721 accgtaagga ttattggcag agtattctgg tcatcgcata gctggtaacc agtggcctga
     3781 agaaacgttt gggtgaggag gatagatggc gttcactgac cttgaatatc aagcggtcaa
     3841 aaaagaagtt caccaattca ttgaaagcat aaggccgcct gaacatatcc gcaatgaact
     3901 ggatattgtt tatagcatca atgaccaaac gatagatatc ggcgaacagc gccccgtgtg
     3961 gcagggcaac ccaggtgaaa caaacatcct gccatcagca agaatcaagt acatacgttc
     4021 tctggataga tggaaaatct attggatgcg gaaggatatg aaatggcatc agtacagtac
     4081 tgaactttcg ctgactgatg cgcttgagct tgtgcgtgct gacccggatt gctgcttctt
     4141 cggatgagtg aagagacatt ttggtgatgg atagtcgcag aagtgcggcc tttaatgtaa
     4201 tcgaagaaaa gtaatccttg taaaacttga tttgactgta ttgtcacaaa gaaagaaatc
     4261 aacgatcgtt tcttgggtct tgatttgttt tgacgatcaa attagtattg cctcatcaac
     4321 tgtatgaggc ttttttatgc aaaccaaaag ctacaccatc aaagcatttc aggacttctt
     4381 acagatgctt cctgagcttg gtatcgtcaa tgatgctaca gcccgcaatc tccgtgactc
     4441 ttccctgcgc cttcttactg ttcttgggtc ggatttcacc gatgccgata tccgtgatta
     4501 tagcgttgcc aacttagcaa gttcgtacgc agactcagca gaaagcaaac cctctgaatc
     4561 atctttgcag gcatacaaaa gccggatgca aagtgctatt gataaattta tctcctacca
     4621 gaatggtgag attaatgtgt cagttggtga tgtaaataaa aaaaaggagc gtaaaaagat
     4681 ggcacctaag aagaaggcgg tagctgacgt acaggttggt gtaaaaacat ttgaactgcc
     4741 gattccattg cgcggagatc ttatcgtcac cattggcaac ctgcctcgcg acctgacaaa
     4801 agatgaagcc aagcgtatca gtctgattgt ggaatctttt gcgatgattg atggcggaac
     4861 aaaagaataa agccccgagg gaacggggct ttttgggtcg gaaatatatc cagtatttcc
     4921 gaagtgtatg gaggattacc taccgctaca cccttggcaa ccgcaagcca aggatagcgg
     4981 ttctcccaac aaactacaac ctgttcgggt aaacaggctg tatacaaaaa caggagaaaa
     5041 gctatggctc acgatgttaa tcgcgatgac ttcatctacg aacacgacaa gccggtttat
     5101 gtgcgcagtt actgccgcat acgtttcggt gagctcgaac atgttcgtca gcacttccgc
     5161 tcttatccga gctgctgaaa ataactaaat ctcaaacccg gcttccgcgc cgggttttct
     5221 ttgcctcgcg atcgtccccc atatttaaca gccgcaaatg cggtaaacca cgaaccaaca
     5281 cgccagcaat acagcgcccc ccttccaata ccgaccaatc cgcaacattt acaaaaataa
     5341 aataccttta ttatccatca cttataactt atttatcaga atccataaat taagttattg
     5401 accaccgcta taacctaggt tatctttaag ccatcagcag gacgcactac tcaccagggc
     5461 ggtgaatata caacgattcg aatatgaatc tacggcgctg acaaagcgca ataaccaaag
     5521 tgaactttgg ggtgtggtga agggttcatg gacgggaata tgtcgcacgt aaagcggcga
     5581 ggcctgcggg actattgccg aattgaagta ggccgaaaca ggtcaaaatg ggtctcccac
     5641 ctaccacacc accaaagttc atcaggaggt ctatatgaca cgcagaactc agttcaaagg
     5701 caattcacgt tctcgtcgtc gtgagcgttt aaaggcaaag gcattagcta acggcgtgct
     5761 gacccgcgaa gaagcaataa gttcagaagt attacaccgc cctactctaa gcagagcaca
     5821 gattcaggct aaaggtactc acgaaacgcc tgagcgcata gaagacgcta agccaattaa
     5881 gttcatggca caggacgtga tctggcaaca gaaagaatac agacgcaatc tggagcgagc
     5941 ggccattgtg tacgcgaatg agtttggaca taagcaacca gaaactggtg tatgtcttcc
     6001 aaacgtagct ctttacgcgg caggctacag gaaatccaaa cagctgacag caagatgact
     6061 tgtgttggtc gccagaaaat gaaatcaggc agcaaaccac ttatttgaga ggaattaata
     6121 tgtcatcaat ccgcttaaca acgagaataa aagaagtaat agcacgcaac gctttaatta
     6181 agtccggggt gttcactgaa cttgaagaag taacaaagct aaagaaccag cttgcacttg
     6241 atgccagagt tattgcgttt ggcgggaaga agaaaactga ggaagtggat cagttatcat
     6301 ccaggttaat ggctataagt aaagaactta aaaaactggg atgttcattt tactcatgcg
     6361 atgttctttc tacttcaatt tatctggccg tatctggcag aagtgttggc tggtattcat
     6421 acggaaaaga cagcaacggc gaagatatat ggctccctac tccgaccaga gataaatgca
     6481 tgtttgacgc agggcacgaa ataacaaaaa ggtttgatga aatctgcgca ttgcaacaaa
     6541 aacttgaagc caggaaaaag gatatcgaat caaatgtatg ggctgcttta aactcagtca
     6601 caacagttaa gcgacttatt gaagtttggc ctgaaagcaa agaattgcta ccaaaagaag
     6661 cagataaagc aagtacagca cttcctgctt tacgggtaga agatttgaat aagatgattg
     6721 gacttccttc cgaggccgca tagtcggcct ttatttttag cataaacaac agaataaaca
     6781 ctgcactgtg tattcattcc aaggagtgaa tacacggagc aatttaaagc gtaaataagg
     6841 agcacaccat gcaatatgcc attgcagggt ggcctgttgc tggctgccct tccgaatctt
     6901 tacttgaacg aatcacccgt aaattacgtg acggatggaa acgactcatc gacgtactta
     6961 atcagccagg agttcccaaa aatggataaa acacttatgg ctatccagac taaattcact
     7021 atcgccactt ttattggcga tgaaaagatg tttcgtgaag ccgtcgaagc ctataaaaaa
     7081 tggaggtcaa aatgattccg gtagagctgg cgaaaactcc agagttaagt cgattaaaaa
     7141 gagaatatca cattgctgag gctcgttact ggcgtaaagc gggagataaa tcaaagaaac
     7201 aactttgttt atggcaggca caaagagagc gcatgaatga gcgcgagttt ctttcctccc
     7261 catccgaatt accattctga ggcaaattat gggaactgcg acattaatac tcggtgaatc
     7321 tggtaccgga aaatcaacca gtttaagaaa cttaccacca gaaaaaacat taatcataca
     7381 gtgcataaac aaaccgctac cattcccatc aagtgattgg gttaaaataa gcaaggaaaa
     7441 caataacggg aacatataca gaacagataa tactgaaaat attgtaaggg cactaattaa
     7501 tgctccgcat gaaattgtag ttattgatga ttatcaggct gtgatggtta acgaactaat
     7561 gggtaaatgc aatgagagag gatatgacaa atttacctcg atagggaaaa acgcatggga
     7621 tgtgttcagg gctgctggtg aaggggcaga cactcggaga gtttatattc tggcacacac
     7681 gcaaactgat gattttggct atacacgaat gaaaaccgtc ggaaaactag tcgattcgac
     7741 acttgttcct gaaggttatt tcactatcgt gttaagaact cacgtacaga acggaaaata
     7801 ctcattttcc acccagacaa acgggagtga ttgttgtaaa agtccggttg ggatgtttga
     7861 tagtcagttt attgataatg atttgtacga agtagataaa aaaatatgct cttactatgg
     7921 gtttgaaaaa aatgagacgt cttgaggtat ccggcgagag atttggcaat ttagttgtta
     7981 ttggtgatgc aggaatgcgt ggtactcaaa ggttggtaaa ctgtgtgtgt gactgcggag
     8041 tagtttgtgt taaacagctt ggcaacctcc gttcagggca cacaagaagt tgtggttgct
     8101 cacgctcatc tacaacaaaa gcaagaaaaa acaaacatgg catgtatgga acgccaacat
     8161 acaagtcatg gtcttcaatg ttaacaagat gcttcaatga aagaaatagt aaatacaaac
     8221 actatggagg aagaggaata actgtttgtg aacggtggct ggactttaaa ttatttttcg
     8281 ctgatatggg aaaaagacca gacggaacta cattgggaag aattgataac aatggtaatt
     8341 acgaaccatc aaattgcgaa tggcagaaaa gtatcgagca agcaagaaat aagagtaata
     8401 ccgtattatt taaattcaat ggagtgctgg caacaatttc agagcactgc gaaaaaactg
     8461 gattaaaccc aagcacggta agaagcagga tttacacata tcattggtct gtagacaagg
     8521 ccttatcaaa aggataaaag catgagcaac gtgattttta cttataacga agaagcagca
     8581 ctgaccgcag ggcaaggtgg ttttattaac gaaactggcg ctcatatcat taccattact
     8641 gaagcagaac taaagcaatc agagaaagga gccaaattta ttgagttttc tggcgaatcc
     8701 gacgacggac gtaaaatcca atatcttagc gtttgtgttc agaaaaatga cggaacggaa
     8761 aacaagtttg gtgcaaacgt cgttcatgcc atgatggggt gtgttgggat taagcagtta
     8821 acgcaacata tggtttccgc cagtaaattt gttgctcctg aatttcatgg aaagaaaatc
     8881 gggttagtgc tccagaaagt attaaccaca aacaaaaaga ctggcgcaga cagctaccag
     8941 atggaaatac gcatcccgtt tattgcacaa acaggtcaaa cccttaaaga aaagtcggaa
     9001 ggcaagcaac cagaaactat cgccaacatg gttgccagcc tcaaagataa agacaatcgc
     9061 gctaaaaacg taagccagaa tcatgcagat gattatggtt acagccagaa cgattaccct
     9121 cctttctgat tactgaaaat aaggctccca ttatgccagc gcctctgtat ggtgcggatg
     9181 acccgcgcaa ctgctccggt agctccaagg cggaggtgct ggaaaatatc aaaaacaatt
     9241 tcgacgcgtt tcttgctctg acaccagaaa caaaagcaga acggatgtac cgacgcgata
     9301 tacaactcgc gctaaaacag gagaaggacc gaacaaacga aacagcaatg agaccgttgc
     9361 gaaaagcgac aatagacaaa ttccctgaat atatcgaccc gcgcctgcgt aattaccgct
     9421 cacgctatgg cgctatcagt aatgactgag gaatttacca tgagaggact tgcatacaat
     9481 cccggcattc ttccggcaga aatgattatt cgccaacgcg taaagccaat gccatcgaga
     9541 gaggaattgc ttaagcgaaa tagtttcggt tctgttaatg acaacaaata tctgaatgcg
     9601 atgtggcgga gtgggaagaa atgaaacaaa tgtcactaat tgagatggat ggatttctga
     9661 aaggtaaatg catcccacga gatttaaagg ttaacgaaac aaacgctgaa tatctggtgc
     9721 gtaaattcga tgaagtacgt gctgaggctc gcaacgaggg tattaactat accgcaagcc
     9781 gtcttgctgc tgcgttcaat cacggattta tcaataagtc tttgcgtgaa gttttcgacg
     9841 ttacacgcat gattttgtca gcgaaagaag agttggctaa tgaacagcat ccgattgatg
     9901 gcctgtctgg tggatatgcg gagaaatccc tagaagaatg ggcggaacag attcgcaaag
     9961 gaggcaacca gtgagcaaga ttgactatca ggcactgcgt gaggcggcag agcaggcaac
    10021 taaagggagc tacatcgtag ggcatacatc tgttaatcgg catggcaatt taacaggagt
    10081 ttttgtttgt caaaaatgga aaggagaacc cggtggtgtg attgcggaat gtcatgttaa
    10141 ctgcctggtt gaaacagatg ctcaggctta tgcaaacgct gaatttattg ctgcttttaa
    10201 tccaaatgtt gcgctggcac tactggatga acgggaaaga aacctgcaat acatcaaaag
    10261 ccgcgatcag gagaacgagg aaattgcgct aacggtaggg aagttgcgtg ttgagctgga
    10321 agcagcagag aacaacctta ttgatagtga atgccatgtt gctgaactgg aagaagcgct
    10381 acgcgataag caggcgttac ttgaagcctc agaaaagcgc atagcagaac tggaggcaca
    10441 gacagttacc gttaaagagg ttggagatgc ttgatagttt tgcgctcgtt gcactggttg
    10501 gcattgtagc gttgatttgg gtgacatccg ggaatcggag gcataagtga acgacaaaga
    10561 actgattaaa gaaatcaaag agcgtatcag cagtttggat gtgcgagaca atgttgaacg
    10621 ccgtgcttat gaaattgctc tggcctcgct gacagcagag ccgataggtg ctttccacat
    10681 tgcagaacag caggtcgatg gcacaagtga ttacatcaag gatggggatt ggcctattga
    10741 taatgggata attgaagtat acgccgcccc tccagcgcca atagtaccgg aagaaaaacc
    10801 aatgcctaat cctcttagca tgtacgcggt tgatgctgtt gccgctattg cagaggtgag
    10861 aggctggaac gcctgtcgtg ccgctatgct tcagagttaa gataattgca ttaaggatgc
    10921 ctggataagc tgtagtgagc gaatgccaga aatgggagag cgacaatgct atgtgttagc
    10981 agctgacttt aaaaacaact acccaccaaa catccccaac actcaggtcg gcgtatatgg
    11041 cgactggttt aatgatggca atcccacttg ggatgacggt gatggcgaag acctgtatct
    11101 caaagaggta acccactgga tgcagctacc agaaccgccg caggaggtta accgtggcta
    11161 acctgcaact tgccgtaaaa ggtgaataac aatcctcgca ttcgcggaga tttcttttat
    11221 ctgaactcgc tacggcgggt tcaccgtcaa gctttcttca atgcaaaagc caatgtgagg
    11281 acgctatgaa tactcaaatt tctgtcgaaa cactttcacc aatcacctac aaacaaacca
    11341 acgtcatcac cagcgaacta ttggcgcagt tgtacggaac ggatgttgtg cgcatccggc
    11401 aaaaccataa tgaaaacaag tcgcgctttg ttgagggggt acacttctac aaggtcgttg
    11461 gtgacgagct gcgcgcgcta aaacacaaag tagctttaaa ctactttgtg aaaattgccc
    11521 caaatgcacg ccacctcatc ctctggacag agcgcggagc cgcccgtcac gcgaagatgc
    11581 tcgaaacgga tcaggcatgg gacgtgttcg aaaaactgga ggacggctac ttcaatcagc
    11641 gcgaaaaggt tgccaaccat cctcaaccag taccgcaacc agaaagtagc ctagaccgtg
    11701 cgcgagccat ggaaatagct ttgaacgtgg cggatcgact ggtgaatatg ttcccgaatc
    11761 tgtcctcaga atcaaagcag gcagcagtag ctggagtgtt caacccgatt gttggtcgcg
    11821 atgttgtacc tctgccagct atctcagagc actactactc tgctacggaa gtcggccagc
    11881 gctacggtat ttcagccaac aaggtaggac gcatagccaa cacctacatg ctcaaaacag
    11941 aaaagtacgg gaagtggttc atcgacaaat cggcgcacaa tgacaagcag gttgaaacat
    12001 ttcggtacaa cgaggctgga gtgcacaaaa tagaggaact catcgaagga gggcgaaagg
    12061 ctgcatgatt ttgacaagat agttttcccc aaatgtgggg aaaagcccga atggcgcggc
    12121 ttacagcaag ataaggccta catgatttga caacaccgca ttaacggggc tatattccgc
    12181 ttcatggtgc tcaaaacacc tttgcgatag cggaaaccgc cccgtcagtt atgcggattt
    12241 ttttatgtct acgacattga gaattcaatg gtcgggcgtg cggctaataa aacacccgca
    12301 agggaaatat gcccgccgtc tatcgccggt tttgagcgcc cgaccaccct ctcaaaaggg
    12361 gtaaattcaa aatatgcgat aggacataaa accatggcta ctcaaatttc tgttgaatcc
    12421 ctttccccaa ttacctataa ccacatcccc gtcatcacta ccgaactact ggcacgactt
    12481 tacggcactg agaccaacaa cattaaagtg aactatacgc gcaatgccgg gcgttttgtt
    12541 tgtggtaagc actactttaa actggagggt ggtgaattgc gggaatttaa gaacaaggtt
    12601 actcacagta acttagttgc accgcgagca aagcacctca tcctctggac agaacgaggc
    12661 gcagcccgcc acgcaaaaat gctcgaaacc gatcaggcgt gggaagtgtt cgaaaaactg
    12721 gaagactgct atttcagccc gggcaaagcg gaacagcacc cgcagatcca gcagcaattc
    12781 acagacgaag aaatcatcct cctttgctat atgcaggtac agatggagaa tgcgctggac
    12841 atctgcaaac gtctgtaccc gatattgaag gaactgaact catcatacac gagcaagctg
    12901 tatgacatcg cgtttgaaac tttttacacg gtgacgaaaa acagagatgt actgctaagg
    12961 gaggcgacac gacttgacca agcaagcgcc cttttcgaac gggcaaaacc aatgctgaaa
    13021 agccttcggg cgagacaatt cgaattttaa tcatcaaagg agcttcggct ccttttttgt
    13081 tggagaaaat aaaccaatac tcgctccctt gcgagtaatt gcggagactt tgcgatgtac
    13141 ttgacacttc aggagtggaa cgcacgccag cgacgcccaa ggagccttga aacagttcgt
    13201 cgatgggtac gcgagtgcag gatattccct cctccggtta aggatggaag agagtatttg
    13261 ttccacgaat cagcggtaaa ggttgactta aatcgaccag taacaggtag ccttttgaag
    13321 aggatcagaa atgggaagaa ggcgaagtca tgagcgccgg gatttacccc ctaaccttta
    13381 tataagaaac aatggatatt actgctacag ggacccaagg acgggtaaag agtttggatt
    13441 aggccgagac aggcgaatcg caatcactga agctatacag gccaacattg agttattttc
    13501 aggacacaaa cacaagcctc tgacagcgag aatcaacagt gataattctg ttacgttaca
    13561 ttcatggctt gatcgctacg aaaaaatcct cgccagcaga ggaatcaagc agaagacact
    13621 cataaattac atgagcaaaa ttaaagcaat aaggaggggg ctgcctgatg ctccacttga
    13681 agacatcacc acaaaagaaa ttgcggcaat gctcaatgga tacatagacg agggcaaggc
    13741 ggcatcagcc aagttaatca gatcaacact gagcgatgca ttccgagagg caatagctga
    13801 aggccatata acaacaaacc cggtcgctgc cactcgcgca gcaaaatcag aggtaaggag
    13861 atcaagactt acggctgacg aatacctgaa aatttatcaa gcagcagaat catcaccatg
    13921 ttggctcaga cttgcaatgg aactggctgt tgttaccggg caacgagttg gtgatttatg
    13981 cgaaatgaag tggtctgata tcgtagatgg atatctttat gtcgagcaaa gcaaaacagg
    14041 cgtaaaaatt gccatcccta caacattgca tgttgatgct ctcgggatat caatgaagga
    14101 aacacttgat aaatgcaaag agattcttgg cggagaaacc ataattgcat ctactcgtcg
    14161 tgaaccgctt tcatccggca cagtatcaag gtattttatg cgcgcacgaa aagcatcagg
    14221 tctttccttc gaaggggatc cacctacctt tcacgagttg cgcagtttgt ctgcaagact
    14281 ctatgagaag cagataagcg ataagtttgc tcaacatctt ctcgggcaca agtcggacac
    14341 catggcatca cagtatcgtg atgacagagg cagggagtgg gacaaaattg aaatcaaata
    14401 atgattttat tttgactgat aatgacctgt tcgttgcaac aaattgataa gcaatgcttt
    14461 tttataatgc caacttagta taaaaaagca ggcttcaacg gattcatttt tctatttcat
    14521 agcccggagc aacctgtgaa cacattttca gtttcccgtc tggcgctggc attggctttt
    14581 ggcgtgacgc tgaccgcctg tagctcaaca ccacccgatc aacgtccttc tgatcaaact
    14641 gcgcctggta cctcttctcg cccgattctg tcggcaaaag aagcgctgaa tttcgatgcg
    14701 caacactatt ttgcatccct gacaccaggt gctgcagcgt ggaatccgtc cccgataacc
    14761 ctgcctgcgc aacctgactt tgttgtcggc ccggcgggta ctcaaggtgt aacgcatacc
    14821 acgattcagg cggcggtaga tgcggcaatt atcaagcgca ccaacaagcg ccagtatatt
    14881 gccgtgatgc ctggtgagta tcagggaacg gtgtatgtcc ctgctgctcc aggtggaatc
    14941 actctgtacg gtacaggtga aaaaccgatt gatgtgaaga ttgggctttc ccttgatggg
    15001 ggcatgagcc ctgccgactg gcgtcacgac gtcaacccgc gcggcaaata tatgccaggt
    15061 aaaccagcgt ggtatatgta cgatagctgc cagagcaaac gcagcgacag tatcggtgtt
    15121 ctgtgttctg cggtcttctg gtcacaaaac aatggactgc aactgcaaaa tctgaccatc
    15181 gaaaacacgc tgggcgatag cgtagatgca ggtaaccatc cggcggtagc actgcgtact
    15241 gacggtgacc aggtacagat taacaacgtt aacattctcg gtcgtcagaa caccttcttt
    15301 gtcaccaaca gcggtgtgca gaaccgtctg gaaaccaacc gtcagccgcg tacgctggtg
    15361 accaacagct atattgaagg ggatgtggat atcgtttctg gtcgcggcgc agtggtgttc
    15421 gataacaccg aattccgcgt ggtgaactca cgtacccagc aagaagcata tgtgtttgca
    15481 ccggctacgc tgtctaacat ctattacggt ttcctcgccg taaacagccg tttcaatgct
    15541 tccggtgatg gcgtggcgca actgggccgc tcgctggatg ttgatgctaa taccaacggt
    15601 caggtggtga tccgtgatag cgccatcaac gaaggtttta acacggctaa accgtgggcc
    15661 gatgcggtga tctctaatcg tccgtttgcg ggtaataccg gcagcgtaga tgataacgac
    15721 gaaatacagc gcaatctgaa tgacactaac tacaaccgca tgtgggaata caataaccgc
    15781 ggcgtgggca gcaaagtggt tgcagaggcg aagaagtaag agctattaac tatttgccgg
    15841 atgcggcgta aacgccttat ccggcctacg gttcgatgcg atttgtaggt cggataagat
    15901 gcgccagcat cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt
    15961 tacatctgac ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct
    16021 tcagccgtca ggctttccat gtaaagcgta atttccgtta ccggcgcatc ttcatggatc
    16081 acataacctt taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa
    16141 atgtaatccc ccacttcaaa ggttggcact tccgccatct gcagcggtaa catcccccag
    16201 ttgatgacgt tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg
    16261 ccaatcacac gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc
    16321 gcatagacca tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc
    16381 tgcttaatgc gcgcaaacac ctctgtcagc tcgctgacat tccccgccag accaatcgga
    16441 tttgaacgat aagaagaggt ttcaccggaa ggaatcagtt cgtcggtggt ggtgacttcg
    16501 tcgaggatct tcgagcacac tttcaggacg atattgtcag tcagcgcacc caattccggc
    16561 cagtctttaa tgttcggtcc gtaaatcagc ggttgctgag ttgccccttt cacaaagccc
    16621 tgataaacac ggtttttata cggcgttaca tcgaaggcgt actccggcac gttgtcccag
    16681 caatcaagtt cgctggcaga ggttaaatag ccaccgtttg ccgcagtcgc agcgatagaa
    16741 cgagcgtcca tcaacgccac cgctgacatc tgcccattgg ctggcttaga gccttcgcgg
    16801 ttcgggaagt tacgcgtggt gtggcgaata ctcaaaccgt tgttgattgg cgtatcgccc
    16861 gcgccaaagc atgggccaca gaatgcggtt ctgatgattg cgcctgcgcc aatcaaatct
    16921 gctaccacac cttttttggc gagatccata aacaccggct gtgatgacgg gtaaaccgcc
    16981 agcgagaagg tgtcattgcc acaggattga ccgcgcagtg cattcgccgc cgcgatgacg
    17041 ttttcgtagt taccgccaga acagcccgcg ataatcccct gctgcacttt caggcgacca
    17101 ttttccactt tatccagcag cgagagtttg gctttaccgt gcgccacgcg ttcggactca
    17161 atttcaatct cacgcagaat gtcggtcagg ttctggttca gtgtgtcgat ttcatacacg
    17221 ttgctcgggt ggaacggcag cgcaatcatt ggtttgatgg cgcttaaatc aacgctgatg
    17281 cagccatcgt agtacgccat cggttgaggg ttaagctggc agtaatcctg gccgcgaccg
    17341 tgcagcgcca gccagttatg cacttcttca tcagtttgcc agacggagct taaacaggtg
    17401 gtttccgtgg tcatcacgtc aacgctgtta cggaaatcgg tagagagcgc gctaacgccc
    17461 ggtccaacga actccatgac tttgtttttg acgtaaccgt ttttgaacac cgcgccaatg
    17521 atagccagcg ccacatcctg tggccccaca tacggcgcag gttttccggt cagatgcacc
    17581 gcaaccacgc ccggatagtc gatatcccag gtgtcattaa gcagctgttt taccaactca
    17641 ccgccaccct caccgactgc cattgtccct aatgcaccgt aacgggtgtg gctgtctgac
    17701 ccgaggatca ttttgccccc gcctgccatc atctcacgca tatattgatg gatgaccgca
    17761 atatgcggag gcacaaaaat accgccataa cgctgagctg ccgataaacc aaaaacatgg
    17821 tcatcaccgt taatggtgcc gccgacggcg cagagtgagt tatggcagtt ggtcagcaca
    17881 tacggcagcg ggaaacgttc cattccggac gctttagcgg tctgtacaat accaacaaag
    17941 gtaatatcgt gagaggctaa tgaatcaaac ttaattttaa gtttatccat atttccggac
    18001 gtattatgag aagagagaat agaccaggca atagtgcctt tttttgcttc ttctttttta
    18061 atttcgccgg tgaaatgttc ttcggcaatt atttcgttat tactggcgag aaacacgcct
    18121 ttttcagata acttgatcat caaatgctcc agatatctgc gcaccgtgaa gtgcgcagaa
    18181 agataataaa tggatatact ctaaataatt cgagttgcag gaaggcgaca agtgagtgaa
    18241 tccccaagag cttacataag taagtgactg gggtgagcga acgcagacgc agcacatgca
    18301 acttgaagta tgacgagtat atttacagca tgttccagcc gatcagcata ttccaccacc
    18361 acacgccgag ggtgatatgc accaggaagg ttaatatcgt cagtaccgca ccgaccaacc
    18421 atcaggattt aatatcgttg taacccacgc caaagatgac cggacctgcc gcgccgccat
    18481 agtgagtaac catgccgcca taggagttgg agaacaacag tgccagcgcg gttaacatta
    18541 acggtgcgcc ggagacgttc gccagcatgg caaatacagg taacatagca acgatatagg
    18601 cactaccgga agcgaagaaa taacgcacga tgatgctgag gaaaataata acgaagaaag
    18661 caacgttacc gtgaccatca aatgccaggt tatttttaaa gacttcagct aaccattcga
    18721 aaaatttaac tttcgataat aaggagctta agccgataat accgccgtac cagattaagg
    18781 tattccagcc gcctttattt ttaaccacgt cttcccaggt aacgataccc agcagcagca
    18841 tggtcgccat aacaacgatt gccacagtgg attcatcaac ccccagagac ttactgaaaa
    18901 tccagcccag tagcgccagc acaaagacac cgagcagcat tttttcgcgg attttcatcg
    18961 gtcccagttc tgcaaggccc gctttagcga tggttttgtt atccaccttt ttaatttctg
    19021 gtggatacat ggtgtaaatt accagcgggg tgaccagcag cataatgatg cccggcaatc
    19081 cggcggccag cgcccatcca ccccagctaa tttgcaggtg cagaatgtcg ttgatcattt
    19141 tcagcgccag aatgttcccc tccattgcgg taaagaacat atagctggtg gttttggtga
    19201 ccatgtaaat ggacatcatc aggtaatgtc cgacacgacg cggacttttt tccggttcgg
    19261 accccaaagc caccgccacg ctgttgatga tcggtaacac aatgccgccc gcacgcgcgg
    19321 tgttagacgg tgttgccgga gccagtacca gatcgaggaa taccgtaacg taacccagac
    19381 ccagcgtggt gttaccgatt ttaccaatca gcagataggc aatacgttta cctaaaccgg
    19441 tggtcacaaa tgcggcgctt aaggtaaacg ccgagaacac cagccaggta gtacctgaag
    19501 agtaaccgct taatacggcg gtggtcttaa acgcaccgtc ggataagtta ccgaccacca
    19561 ccattgaggc agcaacggca attaacagta cgacaggttc cgggaaaggc ttgataacca
    19621 gacccacaat ggccgccagg taaataccaa aaagcaccca cgccagttcg cttaatcctg
    19681 ccggagctgg cataaaacca ataatacatg ggatcgctaa tatcagaatt agcttccata
    19741 acgatttctt attcatttaa aatcctgttc tttctgtatg actcatacag aagtccttta
    19801 ttgattgtcc ttatcctgac aacgaatttt ttcagggaag ataaacttca ccggaaaata
    19861 tttttctggt tgtccgaata acagatgcgc gtaacgtcgt ggcatcctga ccttcattac
    19921 ttaaatgaac gtcgagcgca ccactggggt gttcaatatt gatattgcca tatcctacag
    19981 aagggacgat ttgtcgggtg acggtgcctt ccaatgcaca actactggaa atagcaatag
    20041 caccggttat cgccagcgcg cgatggcaag aatgcggcat aaaataacgc acattaattg
    20101 ccccgccttt ctgcgctgga gaaataagca caggtttagg gataaccata ttactgacat
    20161 cacctaagcc cattgcttta cccgcttgta gccggataga ttcaatgcgg gctaataatg
    20221 ctttgtcggc atccagttcc gccggtaatt cataacctgt ttttcccaga tattcagccg
    20281 gaataatgac gactggcatc gccatatcga tacaggtcac cgggacatcg tcaaaataat
    20341 caatctgatt atcagtcggg aaaacttttc cggttttcgt tccagcggca ttcaggaaag
    20401 tgagcgcaac cggtgcggca gtacccggta cgccgtcaat tctggcgcta ccctcgtact
    20461 cgacaacacc atttggcgtt tgcacatcag cttcgatgaa cgtacccgta ttgacgttgc
    20521 ggatacgtac acgggtaact ggcgaagtcg ctgcaatcaa accattttca atggcgaatg
    20581 cccctacgcc agacagcata ttgccgcagt taggcgtggt atcgacacgt tgctcatgga
    20641 cgataacttg agcaaacaga taatcgacat cagcacgcgg atcgctggaa cggctaataa
    20701 tggcgacttt actggtcagt ggattaccgc cgccaatacc gtcaatttcc agatcgttac
    20761 cggaacccat aattgccatc aatattttat cgcgctgcgt ttgatcttcg ggtaaatgtt
    20821 ccgctaacag gaacgcgccc ctcgaggttc cacctcgcat catcacgcag ggtatttttt
    20881 tcataataat gccctcaatt taaaaacata acttattgcg atatggttac attaaggaca
    20941 aagcatctct aatgacttta gctcaacgat taataacatt ttttcattaa tttaagttgg
    21001 ctaaatgcag ttaaaatttt taacgaccag ccacc
//
LOCUS       DADLSB010000059        20991 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.059, whole genome shotgun sequence.
ACCESSION   DADLSB010000059 DADLSB010000000
VERSION     DADLSB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.059"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..333)
                     /locus_tag="JN331_002131"
     CDS             complement(<1..333)
                     /locus_tag="JN331_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7260276.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRV"
     gene            complement(511..1650)
                     /gene="actP"
                     /locus_tag="JN331_002132"
     CDS             complement(511..1650)
                     /gene="actP"
                     /locus_tag="JN331_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY7260277.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALSLGLGLM
                     FGTAGLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYK
                     DAAGHLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLY
                     ANVFKKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPI
                     ILLSMYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSI
                     SVAFLGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1647..1961)
                     /locus_tag="JN331_002133"
     CDS             complement(1647..1961)
                     /locus_tag="JN331_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY7260278.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2161..4119)
                     /gene="acs"
                     /locus_tag="JN331_002134"
     CDS             complement(2161..4119)
                     /gene="acs"
                     /locus_tag="JN331_002134"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY7260279.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4512..5948
                     /gene="nrfA"
                     /locus_tag="JN331_002135"
     CDS             4512..5948
                     /gene="nrfA"
                     /locus_tag="JN331_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY7260280.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5993..6559
                     /gene="nrfB"
                     /locus_tag="JN331_002136"
     CDS             5993..6559
                     /gene="nrfB"
                     /locus_tag="JN331_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY7260281.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6556..7227
                     /gene="nrfC"
                     /locus_tag="JN331_002137"
     CDS             6556..7227
                     /gene="nrfC"
                     /locus_tag="JN331_002137"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY7260282.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7224..8180
                     /gene="nrfD"
                     /locus_tag="JN331_002138"
     CDS             7224..8180
                     /gene="nrfD"
                     /locus_tag="JN331_002138"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY7260283.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8200..9918
                     /locus_tag="JN331_002139"
     CDS             8200..9918
                     /locus_tag="JN331_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7260284.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9911..10294
                     /gene="nrfF"
                     /locus_tag="JN331_002140"
     CDS             9911..10294
                     /gene="nrfF"
                     /locus_tag="JN331_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY7260285.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10291..10887
                     /gene="nrfG"
                     /locus_tag="JN331_002141"
     CDS             10291..10887
                     /gene="nrfG"
                     /locus_tag="JN331_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY7260286.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11229..12542
                     /gene="gltP"
                     /locus_tag="JN331_002142"
     CDS             11229..12542
                     /gene="gltP"
                     /locus_tag="JN331_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY7260287.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12620..13309)
                     /locus_tag="JN331_002143"
     CDS             complement(12620..13309)
                     /locus_tag="JN331_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7260288.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13403..15550)
                     /gene="fdhF"
                     /locus_tag="JN331_002144"
                     /pseudo
     CDS             complement(13403..15550)
                     /gene="fdhF"
                     /locus_tag="JN331_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(15748..17214)
                     /gene="mdtP"
                     /locus_tag="JN331_002145"
     CDS             complement(15748..17214)
                     /gene="mdtP"
                     /locus_tag="JN331_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY7260289.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTHLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17211..19262)
                     /gene="mdtO"
                     /locus_tag="JN331_002146"
     CDS             complement(17211..19262)
                     /gene="mdtO"
                     /locus_tag="JN331_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAY7260290.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19262..20284)
                     /gene="mdtN"
                     /locus_tag="JN331_002147"
     CDS             complement(19262..20284)
                     /gene="mdtN"
                     /locus_tag="JN331_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY7260291.1"
                     /translation="MESTPKKAPRNKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGRIIS
                     LKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEG
                     KVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIIVDKPDPEMFRIGASAV
                     ANLEPQ"
     gene            complement(20303..20578)
                     /locus_tag="JN331_002148"
     CDS             complement(20303..20578)
                     /locus_tag="JN331_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260292.1"
                     /translation="MPTVLSRMAMHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20787..>20991)
                     /locus_tag="JN331_002149"
     CDS             complement(20787..>20991)
                     /locus_tag="JN331_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallo-beta-lactamase"
                     /protein_id="HAY7260293.1"
                     /translation="RKTLQPQVDASFYISREDLHAVLTGQAKMADLVKAKRAKIIGNG
                     AKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         4719 a         5476 c         5698 g         5098 t
ORIGIN      
        1 aactcttctc gtaattctat cgtcacattt attatttgca tataacgcta tatctataaa
       61 atatttatgt attttcctgt ccgtttcttt cgtgattttt cctgatggta aactatcata
      121 aattcttttc attttctgaa tcaagcctat cactttttca tgatcatcta ttttgaaaag
      181 agagagggct ttattgtcaa ttaacgtgtt tttatgatgt ttgctgtaat tatctgattt
      241 tgttttcttt gtatgtgatg aacttgtgat agagtggtta tcgttaatct ggcaaagagg
      301 gaaaaacata ttatttaaac cattatagcg cataaaatat ccttataatt aacaagaaag
      361 aaaaggcata ttctctgcat tatcattttc tacgactgtc aaaaatcgct cattttttaa
      421 tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat gttggagagg aagagtattc
      481 cccggtcagg cgaccgggga aggggagaga ttaatgtgcg cggccttgct caacgccaaa
      541 gccggtctgg gagcggataa actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc
      601 tgagttatcg gttgccgaga agaaccagat gccgaggaat gccacgctga tagagaacag
      661 cgccgggtat tcatacggga agatggcttt ttcgtgaccg aggatctgta cccaaatagt
      721 cgggccgagg atcatcagca ccactgcggt aatcagcccc agccagccgc ccagcatcgc
      781 gccacgggta gtcagtttcg accagtacat ggaaagcaga atgatcggga agttacagct
      841 cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg ttctgattct caaacagcac
      901 gccgaggata atcgcaatca cgccgaggat cagtacggtg atttttgata cccgcagctc
      961 ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac aagtcatgcg aaaccgccga
     1021 tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata gtggcgaaag caaccgctga
     1081 aataaaaccg aggaataggt tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt
     1141 gttaccacca atcagatggc ccgccgcgtc tttatattcc ggattcgcac caacaagcat
     1201 gatcgcgccg aagccgataa taaaggtcag aatatagaag tagcccataa atccggtggc
     1261 gtagaacacg ctcttacgtg cttcgcgggc atcgctgact gtaaagaagc gcatcagaat
     1321 gtgcggcaag cccgccgtac caaacatcag tcccagcccc agagagagcg cggagatccc
     1381 caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt
     1441 gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta
     1501 ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt
     1561 tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt
     1621 ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta
     1681 ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag
     1741 atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc
     1801 ggcgtgccca gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc
     1861 agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa
     1921 tgcgcattgt cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta
     1981 atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc
     2041 ttgccgaatg taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg
     2101 acgctgtcgc gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg
     2161 ttacgatggc atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc
     2221 ggcaagcgtc gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat
     2281 acggcgcata attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc
     2341 tggcgtcgcc agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc
     2401 tggtgacggt tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat
     2461 attgtgcgga atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga
     2521 ctctatctct gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc
     2581 ggtgatccag taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt
     2641 tttgaaggtg gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc
     2701 ctgaccaggc caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt
     2761 accttcgtta tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc
     2821 tttcagctcg gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca
     2881 ccaggtatcg accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc
     2941 ttccgggtta attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc
     3001 ttcaatcgct ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag
     3061 aatattgacc tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg
     3121 tacgccttca aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact
     3181 gtgtccggta acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc
     3241 aaagacatat ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac
     3301 gccttttggt tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt
     3361 catctcttcc gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc
     3421 gcgcccttcc tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac
     3481 gctggtgacg ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg
     3541 cccggcgcgc acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg
     3601 cccggcaacg gcttccggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg
     3661 ggcgcaggcc agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac
     3721 atcacctttt ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg
     3781 cagctcttta tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc
     3841 ggtgcgatcg ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt
     3901 gccgtcctcg taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt
     3961 ctggtacggt ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac
     4021 gttaatagat tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgc
     4081 gatgttggca ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga
     4141 tgttaataat atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt
     4201 tgggcgacag atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag
     4261 gtcaccaaca aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa
     4321 agaggaagat actgactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag
     4381 acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca tgacagtgta
     4441 ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg
     4501 atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc
     4561 cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa
     4621 ctgtggaagc gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga
     4681 aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca cggctggtga
     4741 tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg
     4801 ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc
     4861 cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag
     4921 acggcgaaga tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca
     4981 acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg
     5041 agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg
     5101 agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat gtggagtatt
     5161 acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa
     5221 atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa
     5281 cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta
     5341 aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac
     5401 tctacaccga ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact
     5461 gccataccca ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta
     5521 acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg
     5581 cgctggatgc aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg
     5641 cccagtggcg ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag
     5701 aaggtctacg gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg
     5761 cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca
     5821 aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg
     5881 acttcatcaa aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa
     5941 gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt
     6001 attacgttcg ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg
     6061 aattaccgct acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg
     6121 taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa
     6181 acatgcttcc gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg
     6241 ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat
     6301 gtacaaggtc ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca
     6361 aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc
     6421 cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg
     6481 cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt
     6541 gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg
     6601 gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg
     6661 cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct
     6721 tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag
     6781 ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc
     6841 gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc
     6901 gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt
     6961 ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt
     7021 cgtaagacca atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag
     7081 gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag
     7141 aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg
     7201 tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg
     7261 tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga
     7321 cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc
     7381 tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct
     7441 tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg
     7501 tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg
     7561 cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga
     7621 tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa acgctgatgc
     7681 tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt
     7741 atccgttcct caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg
     7801 gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg
     7861 aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg
     7921 tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg
     7981 cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga
     8041 ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg
     8101 cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg
     8161 gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa
     8221 gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc
     8281 tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg
     8341 gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc
     8401 gatttctcgg tcatttatgt cgcccaacat agctacagcc tgctgtcgtg ggaactcaaa
     8461 ctggcggcgg tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc
     8521 gcctggagcg cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg
     8581 acgctagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc
     8641 gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg
     8701 caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg
     8761 gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc
     8821 cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc
     8881 ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg
     8941 gaaaacgcct ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg
     9001 acacgccagc gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg
     9061 ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc
     9121 gcactggata acgtccgcgc tgtgccgttg ttcagcctgt ttgcactgat tagccttgcg
     9181 tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg
     9241 ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc
     9301 gtgctggtgg gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc
     9361 gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg
     9421 attgtgctgg cgacgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat
     9481 gctggcgtgc tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc
     9541 agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag
     9601 cgactcgatc tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt
     9661 gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt
     9721 cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg
     9781 ggtgaaaaaa ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc
     9841 tggatctggg ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg
     9901 aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg
     9961 cgctcaggtt gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa
    10021 tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc
    10081 gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga
    10141 agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc
    10201 gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc
    10261 actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg
    10321 ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca
    10381 aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc
    10441 agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca
    10501 caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc
    10561 aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat
    10621 gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc
    10681 gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg
    10741 gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg
    10801 atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca
    10861 aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt cattgagctg
    10921 cataaaaaat aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga
    10981 ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg
    11041 cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc
    11101 gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg
    11161 ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag
    11221 ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc
    11281 tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg
    11341 tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc
    11401 cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg
    11461 ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt
    11521 tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg
    11581 cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg
    11641 gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag
    11701 gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc
    11761 ccgcgacgca tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta
    11821 aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga
    11881 cggtggctaa ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc
    11941 atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt
    12001 taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg tactccactg
    12061 ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg
    12121 cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc
    12181 tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct
    12241 ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg
    12301 ctggcgtgtc gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag
    12361 gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg
    12421 tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca
    12481 aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat
    12541 aattgaagat tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc
    12601 gcgtcttatc aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat
    12661 caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga
    12721 taaaaccttc ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc
    12781 gggaaattgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc
    12841 ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct
    12901 ccagatcttc cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg
    12961 cttgagtatt taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg
    13021 ccgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt
    13081 tgatctgcgc caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc
    13141 aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg
    13201 cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg
    13261 cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac
    13321 gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa
    13381 ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact
    13441 tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc ggctcgacgc
    13501 gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca
    13561 gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac
    13621 gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg
    13681 cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt
    13741 catcagccag cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac
    13801 caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg
    13861 ctacccagtc gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa
    13921 acagataaga agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc
    13981 ccagttcgcc cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact
    14041 catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt
    14101 cactgatgat ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac
    14161 gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg
    14221 gcaaaatgac atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca
    14281 gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat
    14341 cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcaactcgc
    14401 tgatgcgata gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct
    14461 cgcggttagc cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca
    14521 tatcgcaggc accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct
    14581 taccgaggtt accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct
    14641 ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca
    14701 tccgtgccgc ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg
    14761 gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga
    14821 acgctttgtc gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga
    14881 tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc
    14941 gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca
    15001 cgattgggtg ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa
    15061 tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg
    15121 atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg
    15181 caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct
    15241 ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca
    15301 gtgcctcatc tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg
    15361 ttttcaggcg cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct
    15421 tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc
    15481 cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga
    15541 cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt
    15601 atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg gcactattta
    15661 ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg
    15721 cgtgacagcc atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata
    15781 cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat
    15841 cagtaatgac atctcttcgg caagtactgg caaccgggct tcagtggcct gtaagcggct
    15901 ggttaagccg cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac
    15961 gtgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc agatgcgtgc cgttgacggc
    16021 aacgtcacgc accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc
    16081 ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag
    16141 acccgggatg aagttgaact ggcgactggt ttttttgaat aaggtatcca ggtggatggc
    16201 atcaaggccg aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc
    16261 cacctgatct aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc
    16321 gagcaactca taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg
    16381 tttgatctcc ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt
    16441 ttcggtgatt tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg
    16501 cgcgccgtgg aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt
    16561 caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc
    16621 ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt
    16681 ttctgccagc gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc
    16741 ccacaaatca agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc
    16801 gtccatcccc agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac
    16861 acgttggcgg ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa
    16921 atcggcctgc gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc
    16981 acttagcgtc cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg
    17041 cgcctgcggc cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg
    17101 tttgagctgt tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt
    17161 cgtgctgccg agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg
    17221 gctccttgtg cctgttctgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg
    17281 gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag
    17341 gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca
    17401 gcgtatttct ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc
    17461 agcgcctgcg ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga
    17521 ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc
    17581 agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga
    17641 atttgcagat aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta
    17701 cttaacatac ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc
    17761 cagacaaagg tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga
    17821 tcgcgaattt ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg
    17881 aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt agcaatccat
    17941 gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag
    18001 ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa
    18061 cgcagcacca tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc
    18121 agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa
    18181 caggcgagca atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg
    18241 accattgatg gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt
    18301 tgtaacaacg tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt
    18361 tcactgatcc gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg
    18421 ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag
    18481 gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc
    18541 caccatgcgc tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt
    18601 tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc
    18661 aggtggctaa tggcatcatc aagccgatca ttaagcgctt gatgcatttg cgtaatggca
    18721 cgactgggaa accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca
    18781 acgatacacc acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag
    18841 gtttgcccgt aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg
    18901 cgcatcaaaa acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt
    18961 tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc
    19021 acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc
    19081 actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc
    19141 aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac
    19201 tcttcatgaa agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc
    19261 attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag
    19321 gtttatcgac tatgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac
    19381 gagacacttt tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc
    19441 caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg
    19501 cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc
    19561 agtgacgagt gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg
    19621 aggttttgag ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat
    19681 gtagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc agcgcatcga
    19741 cgccgctggc ggctgactgc gcctgtaaca atacggcatt aagatccgct tctgcggcgc
    19801 gctgcgccgt tcttgcacgg tcaacgtctt ctgctgagac aaaaccctct ttcagtaatg
    19861 gctcggtgcg gcgtaatgta tctgtggcct gtttcgcggc ggcacgggct ttttctaccg
    19921 tggcattaac cgagtcggca ccaaactgtt gggcgtcaac gctacgctgg gtgagcataa
    19981 tttgcttatc cagcgccgcg agggaggctt cggctttcgc caggttggct tcgtacgggc
    20041 gcgggtcgat gcggaacagc aaatcgccct gtttcaccgc ctggttgtcg gtgaccgcca
    20101 gttctacaat gcgaccgctg acttccggca ccacatcaat ggtatctgct gacgcgtaag
    20161 cgtcattagt tgatggcgca ctgtctacgc gccagataac gaaaacaagg gcaaccagcg
    20221 ccaacgcaac cactaacaga gcagggaatt tattgcgagg agcttttttc ggcgtacttt
    20281 ccatgagcgc gtctcaaata gattagaaaa atgccagcca gaacagcatg gcgtagagag
    20341 caaaaagggc ggtataaata attccgacaa atgccagatt gatatttgcc cgctgaataa
    20401 ctcgcctcgt gatgagcgtc aaaataaggc tggcaatggc gcagaaaaac cagctgggat
    20461 aataagcgcc gatcaccggg attgccggag ataatgagca tcccgaaacc atgaagacgg
    20521 gaattatcca ggctgttttt ttgaggtgca tcgccatgcg agagagaact gttggcatat
    20581 taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa tatcaatatc cattgaaggc
    20641 gtcagtatgg ctattttctc gatgaataaa aatagcttta tttagccacc gcattcataa
    20701 cttatatatt ttatttatta attggctgta tatattttta aaataaatgc ttttgggggc
    20761 ttcagcgccc ccaaaagcga gtgacattaa tttggggtta cgatattcac ccacaaatcg
    20821 aaattatcca gacaggcgat aatttcttcc agtttcgcgc cattgccaat aattttggct
    20881 ctcttcgctt tcaccagatc cgccattttg gcctgtccgg tcagcacggc gtgcagatct
    20941 tcacggctga tgtagaaaga ggcgtcgact tgcggttgca gtgttttacg g
//
LOCUS       DADLSB010000060        20689 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.060, whole genome shotgun sequence.
ACCESSION   DADLSB010000060 DADLSB010000000
VERSION     DADLSB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.060"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..68)
                     /locus_tag="JN331_002150"
     CDS             complement(<1..68)
                     /locus_tag="JN331_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7260294.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRR"
     gene            76..576
                     /gene="tnpA"
                     /locus_tag="JN331_002151"
     CDS             76..576
                     /gene="tnpA"
                     /locus_tag="JN331_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY7260295.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(615..962)
                     /gene="grxD"
                     /locus_tag="JN331_002152"
     CDS             complement(615..962)
                     /gene="grxD"
                     /locus_tag="JN331_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY7260296.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1297..2124
                     /locus_tag="JN331_002153"
     CDS             1297..2124
                     /locus_tag="JN331_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY7260297.1"
                     /translation="MARINRISITLCALLFTTLHLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEIYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2252..2833
                     /gene="sodB"
                     /locus_tag="JN331_002154"
     CDS             2252..2833
                     /gene="sodB"
                     /locus_tag="JN331_002154"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY7260298.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2979..4148)
                     /locus_tag="JN331_002155"
     CDS             complement(2979..4148)
                     /locus_tag="JN331_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7260299.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGSPLMTLLLSHRARRSALIFLMAIFTPGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFLGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4314..4403)
                     /gene="ynhF"
                     /locus_tag="JN331_002156"
     CDS             complement(4314..4403)
                     /gene="ynhF"
                     /locus_tag="JN331_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY7260300.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4702..5727
                     /gene="purR"
                     /locus_tag="JN331_002157"
     CDS             4702..5727
                     /gene="purR"
                     /locus_tag="JN331_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY7260301.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5724..6656)
                     /locus_tag="JN331_002158"
     CDS             complement(5724..6656)
                     /locus_tag="JN331_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7260302.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6769..7980
                     /locus_tag="JN331_002159"
     CDS             6769..7980
                     /locus_tag="JN331_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY7260303.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8271..9419
                     /gene="cfa"
                     /locus_tag="JN331_002160"
     CDS             8271..9419
                     /gene="cfa"
                     /locus_tag="JN331_002160"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY7260304.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9459..10100)
                     /gene="ribE"
                     /locus_tag="JN331_002161"
     CDS             complement(9459..10100)
                     /gene="ribE"
                     /locus_tag="JN331_002161"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY7260305.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10315..11688
                     /gene="mdtK"
                     /locus_tag="JN331_002162"
     CDS             10315..11688
                     /gene="mdtK"
                     /locus_tag="JN331_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY7260306.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTATRTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11729..12985)
                     /locus_tag="JN331_002163"
     CDS             complement(11729..12985)
                     /locus_tag="JN331_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260307.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13293..13369
                     /locus_tag="JN331_002164"
     tRNA            13293..13369
                     /locus_tag="JN331_002164"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13327..13329,aa:Val,seq:gac)
     gene            13374..13450
                     /locus_tag="JN331_002165"
     tRNA            13374..13450
                     /locus_tag="JN331_002165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13408..13410,aa:Val,seq:gac)
     gene            13558..13863
                     /locus_tag="JN331_002166"
     CDS             13558..13863
                     /locus_tag="JN331_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY7260308.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13989..15530
                     /locus_tag="JN331_002167"
     CDS             13989..15530
                     /locus_tag="JN331_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY7260309.1"
                     /translation="MKKIAIVGAGPTGIYTLFSMLANIASIEIPPINCTYLEWLQKQE
                     ASHLQRYGVKKETLHDRQFLPRILLGEYFRDQFLRLVDQARQQKFAVAVYESCQVTDL
                     QITNAGVMLATNQDLPSETFDLAVIATGHVWPDEEEATRTYFPSPWSGLIEAKVDACN
                     VGIMGTSLSGLDAAMAVAVAVAVAVAVAVAIQHGSFIEDDKQYVVFHRDNASEKLNIT
                     LMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKGEEGLLDRVFRLIVEEIKFADP
                     DWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNLQEVERNKREKHTVPWRYVILR
                     LHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPSESIRRLLALREAGIIHILALG
                     EDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKVKDIPFPGLREQLQKTGDEIPD
                     VGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTACAEIGEAMARAVVKPASRARR
                     RLSFD"
     gene            complement(15542..16354)
                     /locus_tag="JN331_002168"
     CDS             complement(15542..16354)
                     /locus_tag="JN331_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260310.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16358..17143)
                     /locus_tag="JN331_002169"
     CDS             complement(16358..17143)
                     /locus_tag="JN331_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAY7260311.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(17140..17808)
                     /locus_tag="JN331_002170"
     CDS             complement(17140..17808)
                     /locus_tag="JN331_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7260312.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAYIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17872..18510)
                     /locus_tag="JN331_002171"
     CDS             complement(17872..18510)
                     /locus_tag="JN331_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAY7260313.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18523..20625)
                     /locus_tag="JN331_002172"
     CDS             complement(18523..20625)
                     /locus_tag="JN331_002172"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HAY7260314.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPLGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLIPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGKEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKPMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
BASE COUNT         5135 a         5377 c         4858 g         5319 t
ORIGIN      
        1 cgcctcattt cacgctcctg ttgaccaccg ggtcttaact ggaatttaaa tgcctgtaat
       61 cgtttcacag gtcaaatgat actttggtct gtgaaaaaag aaaccgatat ccggcgtgga
      121 aggcattgtg ttttcctgat gcatgttcac ctggtctttg tcaccaaata ccgacgccag
      181 atttttgacc atgacacgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat
      241 tttgcagctg aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac
      301 tatcctccca aactggcgat atccagtctg gtaaacagcc tcaaaggcat atcgaatagg
      361 ttactgcgac gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg
      421 agtcctggct attttgccag tagctgcgaa ggtgcgccaa tatccgtcat ccgccaatac
      481 attgaacaac agcaaacacc tggtcaggtg gaaaaccgcg ccttatatcc ccgccctgaa
      541 ggacgaggtt ttacggcgca ccggataaag gactgacgga tgcccgttcg catccgtcag
      601 tattgcagga cggattattc cgcgtccggc tcttcagact tgtatttagc ggcagtttct
      661 ttgatcagct gctgcagttc gccacgctga tacatttcga tcacgatatc acaaccgccg
      721 accagctcgc cgtcaaccca cagttgcggg aaggtcggcc agttagcata tttcggcagt
      781 tccgcacgaa tgtccggatt ctgcagaata tcaacatagg caaaacgttc gccacatgcg
      841 gcaagcgcct ggactgcctg ggcagagaaa ccgcagctcg gcagtttcgg tgaacctttc
      901 atgtacagca ggatcgggtt ttcagcaatc tggcgttgga ttttttcgat agtggtgctc
      961 attgtcttgc ttcctcaaac ttctttacgg cagtaatctg acattgtagc gggtcagtgc
     1021 ggcatcggaa aataacattt tcatcacgct tttgctattt tatccctttg ctctatttgt
     1081 tgcatttcaa atattcgttt cttctctgat gcgcggcaaa ctggcgtatt tataaccatt
     1141 tttagctttc actgctgcat ttttttgcgc tcgccaacga aacgtatttt ttaacaataa
     1201 aagctattaa ctttctcttc ttctatgcat tagaatcatc aagttttgta aatcagacgc
     1261 aggcatgata gacctgcctt aacagaggga cgctcagtgg cgcggataaa ccgtatttcg
     1321 atcacgctct gtgctttact ttttaccacc ctgcatttaa cgcctatggc ccatgcttca
     1381 aagcaagcca gggagagttc tgctaccact catatcacca aaaaagcaga taaaaagaaa
     1441 agcacggcaa ccaccaaaaa gacccagaaa gccagccaga aaacagcgaa aaaagccgcc
     1501 agtaaaagta cgaccaaaag caaaaccgct tcttccgtta aaaaatcttc cattaccgct
     1561 tctaaaaacg ccaaaactcg cagcaaacac gccgtcaata aaacggcctc agccagcttt
     1621 accgaaaagt gtaccaaacg taagggctat aaatcgcatt gtgtgaaagt caaaaatgcc
     1681 gcgtcaggaa cccttgccga cgcgcacaaa gcgaaggtgc aaaaagccac gaaagtggca
     1741 atgaataaac tgatgcagca aattggtaag ccatatcgtt ggggcggcag ctcaccgcgt
     1801 accggttttg attgcagcgg cctggtttat tacgcttata aagatttggt gaaaattcgt
     1861 attccgcgta cggcgaatga aatatatcac ctgcgtgatg caggacctat cgaacgtagt
     1921 gagctgaaaa acggcgacct ggtctttttc cgtactcagg gacgcggtac agccgatcat
     1981 gtcggcgtgt atgtcggcaa cggcaaattt attcagtcac cgcgcacagg tcaggaaatt
     2041 caaatcactt ctctcagtga agactactgg cagcgccact atgttggcgc tcgtcgggta
     2101 atgaccccaa aaacacttcg ctaaaacttt accctgttgt tacggcaaca gggtaagttc
     2161 atcttttgtc tcacctttta atttgctacc ctatccatac gcacaataag gctattgtac
     2221 gtatgcaaat taataataaa ggagagtagc aatgtcattc gaattacctg cactaccata
     2281 tgctaaagat gctctggcac cgcacatttc tgcggaaact atcgagtatc actacggcaa
     2341 gcatcatcag acttatgtca ctaacctgaa caacctgatt aaaggtaccg cgtttgaagg
     2401 taaatcactg gaagagatta ttcgcagctc tgaaggtggc gtattcaaca acgcagctca
     2461 ggtctggaac catactttct actggaactg cctggcaccg aacgccggtg gcgaaccgac
     2521 tggaaaagtc gctgaagcta tcgccgcatc ttttggcagc tttgccgatt tcaaagcgca
     2581 gtttactgat gcagcgatca aaaactttgg ttctggctgg acctggctgg tgaaaaacag
     2641 cgatggcaaa ctggctatcg tttcaacctc taacgcgggt actccgctga ccaccgatgc
     2701 gactccgctg ctgaccgttg atgtctggga acacgcttat tacatcgact atcgcaatgc
     2761 acgtcctggc tatctggagc acttctgggc gctggtgaac tgggaattcg tagcgaaaaa
     2821 tctcgctgca taataactga tggcaaatgc agcattgcct gatgcgcttc gcttaccatg
     2881 ccaacgcgga tgaggcgtta acagcgcatt cggcaagata aatcgcacgt tgtcagcaac
     2941 tgtaacgcag aaggttatcc ttctgcgttt ttgtttaatt agctgttagc aacgcaaact
     3001 gtttcaggtt gttttctggc tgacataaac accagcaata atgccagtcc cgcgacaatc
     3061 gcccccgtca ccggcacaaa gctgtatccc agcccagcgg aaattaccgc accaccagca
     3121 gctgctccca gcgcatttcc aagattaaag gcaccaatat tgactgatga agacagacct
     3181 ggcgcttcac tggcgacatg catcacgcgc atctgtaacg gaggtacaac cgcaaaggtt
     3241 gctgcgcccc acaccaccat gctaatagct gcgccgaact cattgcgggc caggaacggg
     3301 attgccagca taatcaccat cagcagcaac aaaaagcctt tcaacgtgcc gttaactgaa
     3361 cgatctgcca gtttgccgcc gagatagtta ccgatagaga atccgacacc aatcagcacc
     3421 agcattgccg tgacgaacac cggtgttgcg tgggtaatac tttgcagtac cggagagata
     3481 taggtgtaga gggtaaacat tgcaccagct cccagtaccg tcgtcagcaa tgcagacagc
     3541 acctgcggac gcattaatac cgccagctct tttttcactt caggtcgtgc ccctgtacca
     3601 cctttaggta atgagaagaa cagacttacc attgaaatca ctcccagccc cgccgttgcc
     3661 agaaatgaca tccgccagcc gatggtttca cccaaccagg tcgccgccgg cacgccaccg
     3721 atatttgcca gggttaaccc cataaacata gtggcaactg cgctggcctg tttatgtttt
     3781 ggcaccacgc ttgcggccac gactgaaccc aaacccaaaa atgctccgtg attcaggctg
     3841 gtcaaaatac gtgaaagcat cagggtcata taatccggcg cgatggcgga aagaacgttg
     3901 cctggcgtga aaattgccat caggaaaatc aacgcactgc ggcgggcacg atgagaaagt
     3961 agaagcgtca tcagcggcga gcccaccatt acgccaactg cataggcact gattaacatt
     4021 ccggcagcgg gaatcgagac atccacaccg cgcgcaatga cgggcaacaa gcccattggc
     4081 gagaactccg ttgtcccgat accaaacgcg ccaatcgcca gcgccagcaa cggatagtta
     4141 attttcatgc cttatctcca cctcttcgcg tcattacgcg atattcatta aagtggcgaa
     4201 agcatgacag caatcacaaa aaaatgaaaa taacaaaaag agaaaacact tttgccattt
     4261 tgctaacaaa caggaaggag atgcgaggga gaacgcgctc cctcgagagg aaatcagtgc
     4321 agcgcggcag tcaaacccac ggctacgatc aaaccgagga cgataatcgt tgttaccagt
     4381 gaaaatttaa ggtcggtgct catcaagttt tctccttttt tattaccaca caaaaagtga
     4441 tattacgcat ttttacacac tgtgatgaaa aaatctcccg tcatttataa tgataagtgt
     4501 ttttaccact tccccttttc gtcaagatcg gccaaaattc cacgcttaca ctatttgcgt
     4561 actggccatt gaccccttcc tgacgctccg tgtcgttttt ccggcgtacc gcaacacttt
     4621 tgttgtgcgt aaggtgtgta aaggcaaacg tttaccttgc gattttgcag gagctgaagt
     4681 tagggtctgg agtgaaatgg aatggcaaca ataaaagatg tagcgaaacg agcaaacgtt
     4741 tccactacaa ctgtgtcaca cgtgatcaac aaaacacgtt tcgtcgctga agaaacgcgc
     4801 aacgccgtgt gggcagcgat taaagaatta cactactccc ctagcgcggt ggcgcgtagc
     4861 ctgaaggtta accacaccaa gtctatcggt ttgttggcga ccagcagcga agcggcctat
     4921 tttgccgaga tcattgaagc agttgaaaaa aattgcttcc agaaaggtta caccctgatt
     4981 ctgggtaatg cgtggaacaa tcttgagaaa cagcgggctt atctgtcgat gatggcgcaa
     5041 aaacgcgtcg atggtctgct ggtgatgtgt tctgagtacc cagagccgtt gctggcgatg
     5101 ctggaagagt atcgccatat cccaatggtg gtgatggact ggggtgaagc aaaagctgac
     5161 ttcaccgatg cggtcattga taacgcgttc gaaggcggct acatggccgg gcgttatctg
     5221 attgaacgcg gtcaccgcga aatcggcgtc atccccggcc cgctggaacg taacaccggc
     5281 gcaggccgcc ttgccggttt tatgaaggcg atggaagaag cgatgatcaa ggtgccggaa
     5341 agctggattg tgcagggtga ctttgaacct gaatccggtt atcgcgccat gcagcaaatc
     5401 ctgtcgcagc cgcatcgccc tactgccgtc ttctgtggtg gcgatatcat ggcaatgggc
     5461 gcactttgtg ctgctgatga aatgggcctg cgcgtcccgc aggatgtttc gctgatcggt
     5521 tatgataacg tgcgcaacgc gcgctatttt acgccggcgc tgaccacgat ccatcagcca
     5581 aaagattcgc tgggtgaaac agcgttcaac atgctgttgg atcgtatcgt caacaaacgt
     5641 gaagaaccgc agtccattga agtgcatccg cgtttgattg aacgccgctc cgtggctgac
     5701 ggcccgttcc gcgactatcg tcgttaatca cccgttgcgg gagtctcttc cggctcccgc
     5761 agccactcct tattcagcgt ctcactatcg ccgagatact caagcagcca ggttaacgca
     5821 ggcgacatat cattttgctg ccatgtcaga caacatgccg aatccgggaa ggggttttcc
     5881 agttcaagtg ctacccactt cccctcatta agccacggtt tggcgaaatg tgttggcacc
     5941 atccctatgc ataatcctgc cgagatacag gttgccgatg attcccagtc aggtacgaca
     6001 agtctttttt ggttatccag caaccaggta atacgtttag gtagcgttcg cgaggtgtct
     6061 tcgcgcacca acgacggcca gttgcgcaac gtatcatcgc tgaacgggcc atccatcaac
     6121 gccagcgggt ggtgactggc aacaacgcaa ctccagctta gcatccccat atcccggaag
     6181 gcataacgac cgcctaccgg aatcgcgcgt gttgcgccaa tcgccagttc cacgcgcccg
     6241 tcggaaagcg catcccagac accgttgaac acttcctgaa agacaagaag ttcgacatca
     6301 tcaaaatggc gataaaaatc aacgatcatc tgccgtgtac gttctggcct gacaatatta
     6361 tccactgcga tagctaactg accgcgccag ccgttcgcta tctgctgaca ttgctggcgg
     6421 gtgatctgca tttttttgac aacagagcgc ccttctttga gaaaccacgc tccagcagcg
     6481 gtcagctcca catcacggtg ccgtcgttca aagagcggta ccgccagcca ctcttccagc
     6541 tgacgcacgg tatagctgac cgcagaagga acgcgatgca gctcctgtgc cgcagcgcta
     6601 aaactaccat tacgcgctac cgcatcaaca acttcgagtg aatattctga ccacatagtc
     6661 tgcctgcaaa atttttgaaa ccagtcatca aatattaccg tttcacaaca ctaatttcac
     6721 tccctacact ttgcggcggt gtttaattga gagatttaga gaatatacat gcaacctggg
     6781 aaaagatttt tagtctggct ggcgggtttg agcgtactcg gttttctggc aaccgatatg
     6841 tatctgcctg ctttcgccgc catacaggcc gacctgcaaa cgcctgcgtc tgctgtcagt
     6901 gccagcctta gtctgttcct tgccggtttt gccgcagccc agcttctgtg ggggccactc
     6961 tccgaccgtt atggtcgtaa accggtgtta ttaatcggcc tgacaatttt tgcgttaggt
     7021 agtctgggga tgctgtgggt agaaaacgcc gccacgctgc tggtattgcg ttttgtacag
     7081 gctgtggggg tctgcgccgc ggcggttatc tggcaagcat tagtgacaga ttattatcct
     7141 tcacagaaag tcaaccgtat ttttgcgacc atcatgccgc tggtgggtct gtctccggca
     7201 ttggctcccc tgttaggaag ctggctgctg gtccattttt cctggcaggc gattttcgcc
     7261 accctgtttg ccattaccgt ggtgctgatt ctgcctattt tctggctcaa acccacgacg
     7321 aaggcccgta acaatagtca ggatggtctg acctttaccg acctgctacg ttctaaaacc
     7381 tatcgcggca acgtgctgat atacgcagcc tgttcagcca gtttttttgc atggctgacc
     7441 ggttcaccgt tcatccttag tgaaatgggc tacagcccgg cagttattgg tttaagttat
     7501 gtcccgcaaa ctatcgcgtt tctgattggt ggttatggct gtcgcgccgc actgcagaaa
     7561 tggcaaggca agcagttatt accgtggttg ctggtgctgt ttgctgtcag cgtcattgcg
     7621 acctgggctg cgggcttcat tagccatgtg tcgctggtcg aaatcctgat cccattctgt
     7681 gtgatggcga ttgccaatgg cgcgatctac cctattgttg tcgcccaggc gctgcgtccc
     7741 ttcccacatg caaccggtcg cgccgcagcg ttgcagaaca ctctgcaact gggtctgtgc
     7801 ttcctcgcaa gtctggtagt ttcctggctt attagtatca gcacgccatt gctcaccacc
     7861 accagcgtga tgttatcaac agtagtgctg gtcgcgctgg gttacatgat gcaacgttgt
     7921 gaagaagttg actgcccgaa tcatggcaat gccgaagtcg ctcatagcga atcacactga
     7981 tctatatcga tatacttata cttaggctgc taacaaaatt ttgttgtatg attgaaatta
     8041 gcggcctata ctaatttcga gttgttaaag ctacgataaa tattatgttt ttacggggac
     8101 aggatcgttc ccgactcact atggatggtc atttcggcaa gggttcctcc tttccctctg
     8161 ttctacgtcg gattatagac tcgcggtttt ttctgcgaga tttctcacaa agcccaaaaa
     8221 gcgtctacgc tgttttaagg ttctgatcac cgaccagtga tggagaaact atgagttcat
     8281 cgtgtataga agaagtcagt gtaccggatg acaactggta ccgtatcgcc aacgaattac
     8341 ttagccgtgc cggtatagcc attaacggtt ctgccccggc ggatattcgt gtgaaaaacc
     8401 ccgatttttt taaacgcgtt ctgcaggaag gctctttggg gttaggcgaa agttatatgg
     8461 atggctggtg ggaatgtgac cgactggata tgttttttag caaagtctta cgcgcaggtc
     8521 tcgagaacca actcccccat catttcaaag acacgctgcg tattgccggc gctcgtctct
     8581 tcaatctgca gagtaaaaaa cgtgcctgga tagtcggcaa ggagcattac gatttgggta
     8641 atgacttgtt cagccgcatg cttgatccct tcatgcaata ttcctgcgct tactggaaag
     8701 atgccgataa tctggaatct gcccagcagg cgaagctcaa aatgatttgt gaaaaattgc
     8761 agttaaaacc agggatgcgc gtactggata ttggctgcgg ctggggcgga ctggcgcact
     8821 acatggcatc taactatgac gtaagcgtgg tcggcgtcac catttctgcc gaacagcaaa
     8881 aaatggctca ggaacgctgt gaaggcctgg atgtcaccat tttgctgcaa gattatcgtg
     8941 acctgaacga ccagtttgat cgtattgttt ctgtggggat gttcgagcac gtcggaccga
     9001 aaaattacga tacctatttt gcggtggtgg atcgtaattt gaaaccggaa ggtatattcc
     9061 tgctccatac tatcggttcg aaaaaaaccg atctgaatgt tgatccctgg attaataaat
     9121 atatttttcc gaacggttgc ctgccctctg tacgccagat tgctcaatcc agcgaacccc
     9181 actttgtgat ggaagactgg cataacttcg gtgctgatta cgatactacg ttgatggcgt
     9241 ggtatgaacg attcctcgcc gcatggccag aaattgcgga taactacagt gaacgcttta
     9301 aacggatgtt tacctattat ctgaatgcct gcgcaggtgc tttccgcgcc cgtgatattc
     9361 agctctggca ggtggtgttc tcacgcggtg ttgaaaacgg ccttcgagtc gctcgctaaa
     9421 ggctattcta tcgccccctc ttcgggggcg agttcagatc aggcttctgt acctggttga
     9481 ttcatggcat tttctcgtgc cgccagcaca cgttctaccg tatctaccac tgcctgagtt
     9541 tgtggatcga tttcaatgtt gacgcgtgcg ccaagttttt tcttcccaag agtcgtgcgt
     9601 tccagtgttt ccggaattaa atggacgcaa aaacgcgttg gcgtgacttc gccgacggtc
     9661 aggctaatac cgtcgatgcc aataaatcct ttgtacagga tatatttcat caactgacta
     9721 tcctggactt taaaccagat ctggcgatta ttttccgagg ttaatatttt cgccacttca
     9781 gcggtggtca taatatgacc tgacattaag tgtccgccaa tttcatcact gaatttcgcc
     9841 gcacgctcaa cgtttaccca atcccccact tttaaatcgc caagattggt aatgcgtaac
     9901 gtttctttca tcaggtcaaa actgacatgg ttgccgttaa tttccgtcac ggtcaggcag
     9961 caaccgttat gcgccacgga agcaccggtt tccaggccgt ccagcatgtg gtcgggtaac
    10021 tccaccacat gcgtacgaaa atttggtttc tcgtcaatcg acaccagttt tgcggtgccc
    10081 tgtacaatcc ccgtaaacat acttacaact cctgaaatca gttaagacat tctgttcagc
    10141 acaatagcag gtggaaaacg cccttaccag tgaaggggta agaatggcta ttttttcact
    10201 ggagaattaa taaatcctcg ctacaataga ctgaatttcc cctgcttctt ctttttgctg
    10261 cccattcagg cggcttttta gtctctcata taactacaaa taaaaggtgt tcacgtgcag
    10321 aagtatatca gtgaagcgcg tctgttatta gcattagcaa tcccggtgat tctcgcgcaa
    10381 atcgcccaaa ctgcgatggg ttttgtcgat accgtgatgg cgggcggcta tagtgccacc
    10441 gacatggcgg cggtcgctat cggtacttct atctggcttc cggcgatcct ctttggtcac
    10501 ggactgctgc tggcattaac gccggttatc gcgcaattaa atggttccgg tcgacgtgag
    10561 cgcattgcgc atcaggtgcg acaaggtttc tggctggcag gttttgtttc cgttctcatt
    10621 atgctggtgc tgtggaatgc aggttacatt atccgctcca tggaaaacat cgatccggct
    10681 ctggcggaca aagccgtggg ttatctgcgt gcgttgttgt ggggcgcgcc gggatatctg
    10741 ttcttccagg ttgcccgtaa ccaatgtgaa ggtctggcaa aaaccaagcc gggtatggta
    10801 atgggcttta tcggcctgct ggtgaacatc ccggtgaact atatctttat ttatggtcat
    10861 ttcggtatgc ctgagctcgg tggcgttggt tgtggcgtgg ctactgcggc ggtgtattgg
    10921 gtcatgttcc ttgccatggt ttcttacatt aaacgcgccc gctccatgcg cgatattcgt
    10981 aacgaaaaag gcaccgctaa gcccgatcct gcggttatga aacgactgat tcaactcggt
    11041 ttgccgattg cgctggcact gttctttgaa gtgacactgt ttgccgtcgt ggctctgtta
    11101 gtgtctccgc tcggtattgt tgatgtcgca ggacaccaga ttgccctgaa ctttagttca
    11161 ctgatgtttg tgcttccaat gtcgctggcg gcagcggtaa ctatccgcgt aggttatcgt
    11221 ctgggtcagg gctcaacgct ggatgcgcaa accgctacgc ggaccgggct tatggtgggt
    11281 gtctgtatgg caaccctgac ggccattttc acggtttcac tgcgggagca aatcgccctg
    11341 ttgtacaacg acaatcccga ggttgtaacg ctggctgcgc atttgatgtt gctggcggcg
    11401 gtatatcaga tttctgactc aatccaggtg attggcagtg ggattttgcg tggttataaa
    11461 gatacgcgtt ccattttcta tatcaccttt acagcttact gggtgctggg cttgccaagc
    11521 ggctatattc tggcactgac cgatctggtc gttgaaccta tggggccagc aggcttctgg
    11581 ataggcttta ttatcggcct gacatcggca gccattatga tgatgctgcg tatgcggttc
    11641 ctgcaacgta tgccgtcagc catcattctg caacgagcgt cccgctaata aagacaaggc
    11701 gcaaccttca cgggttgcgc ctgtgttttt acgcaggctg gagcgttgcg ccaatcccgt
    11761 cttcgtctgg ctgtaatttc agagcgttac acagagtttg ccgactggca tctgttatct
    11821 caacgcgtat atccccgtta acttcccctt tcacattgat gaagtcaggt gaaatttctt
    11881 tcgcactggt ttgcaggcaa atcgctccct ctccgcgcag gcgtttcact ttcagttgca
    11941 catgcgttgc cgcaccagtg atcagccaca gttttccgcc attcagattg atgttaccaa
    12001 caatgaaagt gacgctgtca ccttttaccg tactgccgtc gagaatcaac acaccgccct
    12061 gctgtacagt aatcgttgat tgttgcagct ctgcgccata accaataatc attgaagcgc
    12121 cgtccagtaa tgcaacactc ccggtcagtt caaccggtgc gcgatcttcc tctacgacaa
    12181 aattttcgtc cgcgctggtg ggtgacaaca ggatcactgt tccaccttgt attaccgcat
    12241 tttccagcgt accggcatgc acttcagccc gaccccccac attcaccgac aggttttgcg
    12301 tcttaccgga actgtagagc tgaaggccat ccgtccccag acgataaatg gatccattct
    12361 caacaatagt gtcagagtct tcaccaccga gattattcat ggtgccacca gcaagcaacg
    12421 tatcactggc tttcccggcc agcataaaaa cgccgccctg attaatctca caattgctgg
    12481 cttctccact tggatacacc aacaattcac caccttcatc taccacgacc gctgaggttg
    12541 tcccattaac caacaggccg ccctcttgat cgagaatgat tttttccgcg cgatgtcctt
    12601 ccagtacacg caggttaccg ccattttcca gcaacaaacc gcaggcataa ccctgatcaa
    12661 cgctgaattc accttcggga tggcggccat taaccgtagc gagcgtagag agactaattg
    12721 cgcctccctc ggactgaaca atatctgtgg caatggcacc gtcgctgacc gatagcgtgc
    12781 cattgttacc gtactgtgtg catttggcga tcccaccggt tgccactttc aaccagccag
    12841 cccccgtgac cacggtattt tcggctcttc cgccagcttc aacaatcaac tcgccttcag
    12901 taagttgcgt cgcgccaatg acctcgccgg tcttaacaat ttgcacattc ccgtcgctca
    12961 tcaagttttt cgcatcagat cccatgattt attcctttgc tgcatctgtg tgcctttatt
    13021 gctacctaag tgtaaaggct acggaggatt tatccacgac agatttgaga tggtggcaaa
    13081 caactctgtt taaactctga tacacgaatt attgggttgt atcagatgta aatgcgatcc
    13141 tgaataaaaa tcgcccttgc aaatcaacaa aatatagcca atttgcttaa aagctctgca
    13201 aacggtcgaa atagcgcaga aaattacgtt ttgcctcttg ccacctttcc actctgccgc
    13261 taatattcgt ccccgttgtc acctacaacg ttgcgttcat agctcagttg gttagagcac
    13321 caccttgaca tggtgggggt cgttggttcg agtccaattg aacgcaccat cctgcgtccg
    13381 tagctcagtt ggttagagca ccaccttgac atggtggggg tcggtggttc gagtccactc
    13441 ggacgcacca gattttctta atctggtctt ttcctttttc cctctgtttc ttctctgtat
    13501 ccaatacgtt aaaagattta cactgtcttc gtatgcgtta tcagaaggag aatcgctatg
    13561 gcaactttgt tacaacttca ttttgctttt aatggcccat ttggcgacgc aatggctgag
    13621 cagcttaaac cacttgctga gtcgattaat caggaacctg gttttctgtg gaaggtatgg
    13681 acagaaagtg aaaagaacca cgaagccggt gggatctacc ttttcactga tgaaaaaagc
    13741 gcccttgcct atctggaaaa acatactgcc cgactgaaaa atctcggcgt tgaggaagtt
    13801 gtcgccaaag ttttcgatgt caatgagcca cttagtcaaa tcaatcaggc aaaactcgcc
    13861 tgacagaatt taatcaaggg cggttagcgc ccttttcatc cctgtctgaa atttctcaaa
    13921 ttctaaaaat ctcaaccaaa cttatctgat aacactaaat tcgaaagaat gcgtacaggt
    13981 aagtaacaat gaaaaaaatt gctattgtgg gtgccgggcc tacggggatc tacaccttat
    14041 tctcgatgct ggcaaatatt gccagtattg aaataccgcc gattaattgt acgtatctcg
    14101 aatggctaca aaagcaagaa gccagccatc tccagcgtta tggcgttaaa aaagaaacct
    14161 tgcacgatcg tcagttttta ccgcgaattc tgctgggcga atatttccgc gatcaatttt
    14221 tacggttagt agaccaggca cgacagcaaa aatttgcagt ggctgtttat gaatcatgcc
    14281 aggttaccga tctgcaaatt acaaatgctg gcgtcatgct cgctacaaat caggatttac
    14341 ccagcgagac gtttgattta gcggtgattg ccacgggtca cgtctggcct gatgaagaag
    14401 aagcaaccag aacgtatttt cccagcccgt ggtcaggcct gatagaagca aaggtcgatg
    14461 cgtgtaacgt gggtattatg ggaacatcct tgagcggact ggatgcggca atggcagtgg
    14521 cagtggcagt ggcagtggca gtggcagtgg cagtggctat tcagcatggt tcgttcattg
    14581 aagatgataa acaatacgtc gtttttcacc gcgataacgc aagtgaaaag ctaaatatta
    14641 cgttgatgtc gcgcacgggg attttacccg aagccgattt ctattgccct attccctacg
    14701 agcccttaca catcgtcacc gatcaggcat taaatgctga gattcaaaaa ggtgaagagg
    14761 gccttttgga tcgggtattt agattgatag tagaggaaat caagtttgct gatccagact
    14821 ggagccaacg catagcctta gagagcctga atgtcgattc ctttgctcaa gcctggtttg
    14881 ccgagcgcaa acaacgcgac ccatttgact gggcagaaaa aaatctccag gaagtcgaac
    14941 gcaataaacg agaaaaacat actgttccct ggcgttatgt cattctgcgc ctgcatgaag
    15001 ccgtacagga aattgttcct catctgaatg aacacgacca taaacggttc agtaaaggcc
    15061 ttgccagggt tttcatcgat aattatgcgg caatcccttc agagtctatt cgtcgactac
    15121 tggccttacg tgaagcgggg atcattcata ttctcgccct cggtgaagac tacgaaatgg
    15181 aaattaatga gtcgcgcacc gtcctgaaaa cggaagacaa cagctactcg tttgacgttt
    15241 ttattgatgc ccgcggacag cgtccgctta aagtgaaaga tattcctttc cctgggctac
    15301 gcgagcaatt acagaaaaca ggggatgaaa tccctgatgt tggcgaagat tatacgttac
    15361 agcaacccga agatattcgt ggacgcgtag cgttcggcgc gttgccctgg ttgatgcacg
    15421 accagccttt cgttcaggga cttacggcat gtgcagaaat tggtgaggcg atggctcggg
    15481 cggtcgtaaa gcctgcatcc cgtgctcgtc ggcgtctttc gtttgattaa aggtaaagct
    15541 atcagcactc aacaaactga tgctgttcat taagacgata gcggactccg gttcgaatat
    15601 tgttttcacc ttcaatggcg acggcgaagc gaactctttc gccgtcatga tatgccagtg
    15661 cggcgcttcc ccctggcccg agaataattg agtcgaccac gcccgtgctc actaccgttg
    15721 aattttcacc gctagcaatg atgtgtacat tgtcgccgct gttggcaatt ctggcgtttg
    15781 cgccaaagct ggcgatttgc accaggtcac cattactggc gacatgacac cgttcaccca
    15841 atgtacaaac gcgtactcgc attccggtat tcgcaatccg actactatct ccagcgctgc
    15901 ttatccgcga agaattaccg gttacagcaa cccgtgcgcg ttcaccggag ctgccaatgt
    15961 gactgttaaa gccgacactt ccaatccgca cgctataacc catactggca atttgcgcgg
    16021 catacccagc ggttgcgatt cgggcgttat cctcacaaca gccgatgcga gcatgatcgt
    16081 cgctgcgggc aaactgactg caaaatggta tctctccggg gtctgtggcc agtttttgca
    16141 tagaggaaac ttcctgatgc gcgaagtttt catccgccag ccattttttc cacgcatact
    16201 caaccagact ctccgcccag tccgtatatc cttcctcaat cagtgcatga cgaatatcgg
    16261 cataactacc gccgcgtggg aaatggcgca ggaaccagcg atacatcacc gctccgatac
    16321 gccattctcg caggtctgct cgtgtaatga tcattcgtta gtgccggtga tagccatcga
    16381 ccatgctttt aaaggtttcg tgtggcgtac gtcccgtggt gcaaagataa agatgaccga
    16441 agataaaaaa gaggcttata aatgccagag caaaatgcgc ctgcaataac cagtatctta
    16501 cgccaggaaa cacatctccc acggcttgcg gatagagaca caacagcccc gtcaatagta
    16561 acaacggcag caatccatac atgacaccaa cataggcgac ctgctgtaag ggattaaatt
    16621 tagactgtgt tgttgccggg aaaggatgtt cttccccctg cataatgcca aacaaataaa
    16681 atcgcgtttg ttttgccgct cgttccagcc acccctgacg acgaatgcga tagtggtgac
    16741 cattatcccc aacggcattg atcagcacaa agccgagcca gcatgccagt aacaaaaatc
    16801 cgcaaacttc atgcaccgca accagacttt taaccgcagt tgcgcccacc atcgcaaagt
    16861 gatttatcag cccacttgcc agcaacaata caaagagtaa cgcattcgac cagtgccata
    16921 atctgaccgc tttgctgtaa agataaacct tctcaccatg atcggtggct gatttcttca
    16981 cgccacgagc acgaagcaag gcgtgcagcc ccagcaccaa ccacatccca accagcaata
    17041 ctccggcaat gatcaaccac accggccaaa attcgggagt gaactgtggc acatagttcg
    17101 ctaactggct ctgaaactgt tcagcatgtt gcgacgggtt catacatttt cctttttaat
    17161 caaatgttga ccgaaccggc gatacagatg cggttttccg gcaccaggta gctgatattg
    17221 atagtattta ttatcctgca accacgcctg aatttctggg ctatcttcgc gaccaaagat
    17281 caatgcgtgt tccggacagg cgctgacgca aatgggcgga aagccttttg ccagtcggga
    17341 ctcagcgcag aaatcacatt tatctgccac tttagtgaca gggttaaggt agcgaacctg
    17401 gtacggacaa gcgccaatgc aataactgca gccgatgcac tgcgattttt ctacccgcac
    17461 gatcccctgc tcatcacgcc acgatgcccc cgtcgggcat acgtcgatgc acggagcatc
    17521 ttcgcagtgc tgacatgact ggcgaaaaaa gtgatactgc gtttcgttgt cgttatcggt
    17581 gactggaatg tatgctatcg ataaacggct tccctgagcg gggacgtggt tagttttacg
    17641 gcatgcgcgg gcgcaaatat tacagccatt gcaccgtgac tcgtcatgaa tcatggcata
    17701 ccgaacttcc ttttcttgcc tcgtgttcgc taacagagaa gacgcagaac cagtaaaaaa
    17761 gattactgtt cccatcccca gaacaaattt gcgtcgagtg aaggacatct gattatcccc
    17821 taatgcagtg gctgcaactg tccaccctat ccatgacatc tccctgaaat ttcaggggtt
    17881 ctgaaactgc tctacctcga accattcgca taagccataa gccttgccat aggtcataaa
    17941 tcgctcgcta aacaagtaca acaccgccgt cgatgactcc acctgccgta tatcggcata
    18001 ttcaggtgca acatccatcg cggcgatcgc ggcttcaatc tgcgcttcct ggaagtaata
    18061 tggcgcttgc atcagcatgg cgactttgta aggacgagga tacgtctggc actcaaaacg
    18121 aacggcgtgt gcaatggcgc gacaaatgtc ctgctccaca acttgcaaag acatcgccgc
    18181 gtagttttca ctcatggcct gcgttgagta atagtaatca tcctgacttc ccgtcataca
    18241 agcgatatcc gcgtagtctc cgttctgctt aatgctttcc agcaaaactg acagttcctc
    18301 ttctgcaacc gaatagggtg gcgagagaaa cacggtgcgc gccaccagct gcccagccgc
    18361 agaatgctgg cgaatgaact cagccaggat ttcagcaggt tctggttcgg ttaccgtatc
    18421 aacggggtga cgcatcccct gcaaccattg ccgcttcgcc tcgtcaactt cgctgacttt
    18481 cgctaacggc aattcatcct gatggttcat cttacccatt ccttacgcag gcagtagatt
    18541 gtgtgctgcc agatcggcgg caatatcttc cagccccaga cgctgcaatg tttcgcgggt
    18601 tggacaacca agctgtgggt cccagcccat ttctttgtag aacatcgtga gcgaagcatg
    18661 catatcgtca cgatccattt tgtcagtacc ttcagtaaag accgggatct gcggatcctt
    18721 gtcgaaaacc caggaacaga taagatcgtg ttcattacgc atatctttgg tttgcatcgg
    18781 ttttaccgtg taggcacgat gcaacgtaaa gatacgctct gcagctaaat ctaatttttc
    18841 ctgagtcatc tcttcgccgg tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc
    18901 ccggtaatta cggcttttca gcggcgaaac ggtcattggc cagacccagt tgcacagggt
    18961 gacggcgtta tgcaaacaga cccgcaacag cgaccacttt gcatatttaa tttttgcgtc
    19021 attgattggc gtgtagtttt tggtttcatc gtaagcatct tcagaaccaa aaagttcttt
    19081 cgccacttca cgttgcagtt tcaatggcaa gccggaacca ataaagttga tatgggtatg
    19141 cgtcatgcag tcacggttga acatacagtt aacaatggaa ccgacctgcg ctgacgcttc
    19201 attggcatgg tgaaccggat agccaaacgg cgaccagagt ttatttttcg catagcccca
    19261 gtactcttta cccaaattcc agcgttctgc gatggcatat gaaccatcag ccaggtgact
    19321 cagctcaccc acacgatgcg ccagacggta gtaaaaatct ttaatgaagt taacgtcacc
    19381 cgcttccagt tgatcccagc gaatttctgc atactcttca gctggcagaa cacgcttgaa
    19441 cacacctttg ctgtaacaat aagtaaagtc gcgatgcaac tgcccgtagt tacaccatag
    19501 gccgtagtca tcgaacaaat tcagaccaac caggttacca atcacacgac catcgtcttt
    19561 atcttcaaaa tctttcggcc cgttcgggaa gatggtggta tggacaaagt ttgctacaca
    19621 agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt
    19681 catacaacgg atcgggcaag agtggcagcc gctcattttt actgtgtatt tctctgccgc
    19741 cggtcctaag tcaaaaacgg atttataggt acgaaagccg accgtattct gattgcctgg
    19801 cggaatttca cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg
    19861 tgctgtccag cgtgacttgg gatctgaata ctctgcccac gattgtggcg tacttggcac
    19921 gacatggttg ttattcgcac caataagttc agtcatcatg taatcattga gacgcttcat
    19981 ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt
    20041 tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat
    20101 gccagagaga ggaataaggt tttccccagc ctgaccaata gccgccacac aggtttccgg
    20161 actggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc
    20221 ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt
    20281 cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc
    20341 catatgggca tcgacgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac
    20401 gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt
    20461 cgcttcatcg aaaggtttcg tgcctagcgg tacttcgtca tacataattt tgtagccgaa
    20521 gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt
    20581 cgtgagattg actcttaata tattacctgt ccaaccgtta gccatgattt ttttctttgc
    20641 aagattcaca cagtaatatc tttccactcg ataattttta acgcccctg
//
LOCUS       DADLSB010000061        20619 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.061, whole genome shotgun sequence.
ACCESSION   DADLSB010000061 DADLSB010000000
VERSION     DADLSB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.061"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..848
                     /locus_tag="JN331_002173"
     CDS             <1..848
                     /locus_tag="JN331_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7260315.1"
                     /translation="VLVVFVGILLLMAFVVLPIAWQQGIYLIRDMPGMLNKLSDFAAT
                     LPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLLTIAVYLVLVPLMVF
                     FLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVLEMIVVGIATWLGFL
                     LFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGTEFWSCFAVYLIIQA
                     LDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPLATLIKAVIHAWPDG
                     QIAQE"
     gene            complement(886..1734)
                     /locus_tag="JN331_002174"
     CDS             complement(886..1734)
                     /locus_tag="JN331_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HAY7260316.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAILHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(1731..3767)
                     /locus_tag="JN331_002175"
     CDS             complement(1731..3767)
                     /locus_tag="JN331_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAY7260317.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEELLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCTAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVRRCCNLLLQEYA"
     gene            complement(3797..4210)
                     /gene="hyfJ"
                     /locus_tag="JN331_002176"
                     /pseudo
     CDS             complement(3797..4210)
                     /gene="hyfJ"
                     /locus_tag="JN331_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
     gene            complement(4203..4961)
                     /gene="hyfI"
                     /locus_tag="JN331_002177"
     CDS             complement(4203..4961)
                     /gene="hyfI"
                     /locus_tag="JN331_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY7260318.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(4958..5509)
                     /gene="hyfH"
                     /locus_tag="JN331_002178"
     CDS             complement(4958..5509)
                     /gene="hyfH"
                     /locus_tag="JN331_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY7260319.1"
                     /translation="MLKLLKTIMRAGTTTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTV
                     THKADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCP
                     ECKQRATLINDDTDVPLVAKEQL"
     gene            complement(5519..7233)
                     /gene="hyfG"
                     /locus_tag="JN331_002179"
                     /pseudo
     CDS             complement(5519..7233)
                     /gene="hyfG"
                     /locus_tag="JN331_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            complement(7223..8803)
                     /gene="hyfF"
                     /locus_tag="JN331_002180"
     CDS             complement(7223..8803)
                     /gene="hyfF"
                     /locus_tag="JN331_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY7260320.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     ARGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGILKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(8808..9458)
                     /gene="hyfE"
                     /locus_tag="JN331_002181"
     CDS             complement(8808..9458)
                     /gene="hyfE"
                     /locus_tag="JN331_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY7260321.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(9470..10909)
                     /locus_tag="JN331_002182"
     CDS             complement(9470..10909)
                     /locus_tag="JN331_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY7260322.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLLGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(10926..11873)
                     /gene="hyfC"
                     /locus_tag="JN331_002183"
     CDS             complement(10926..11873)
                     /gene="hyfC"
                     /locus_tag="JN331_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY7260323.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(11884..13901)
                     /gene="hyfB"
                     /locus_tag="JN331_002184"
                     /pseudo
     CDS             complement(11884..13901)
                     /gene="hyfB"
                     /locus_tag="JN331_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(13901..14518)
                     /gene="hyfA"
                     /locus_tag="JN331_002185"
     CDS             complement(13901..14518)
                     /gene="hyfA"
                     /locus_tag="JN331_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY7260324.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(14771..15241)
                     /gene="bcp"
                     /locus_tag="JN331_002186"
     CDS             complement(14771..15241)
                     /gene="bcp"
                     /locus_tag="JN331_002186"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY7260325.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(15241..15813)
                     /locus_tag="JN331_002187"
     CDS             complement(15241..15813)
                     /locus_tag="JN331_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY7260326.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKSTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15959..16837
                     /gene="dapA"
                     /locus_tag="JN331_002188"
     CDS             15959..16837
                     /gene="dapA"
                     /locus_tag="JN331_002188"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY7260327.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAACDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16854..17888
                     /gene="bamC"
                     /locus_tag="JN331_002189"
     CDS             16854..17888
                     /gene="bamC"
                     /locus_tag="JN331_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY7260328.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            18101..18814
                     /gene="purC"
                     /locus_tag="JN331_002190"
     CDS             18101..18814
                     /gene="purC"
                     /locus_tag="JN331_002190"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY7260329.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18982..19845
                     /locus_tag="JN331_002191"
     CDS             18982..19845
                     /locus_tag="JN331_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY7260330.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNHLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19860..>20619
                     /locus_tag="JN331_002192"
     CDS             19860..>20619
                     /locus_tag="JN331_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7260331.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASD"
BASE COUNT         5184 a         5474 c         5545 g         4416 t
ORIGIN      
        1 ttgtattggt ggtttttgtc ggtatattgc tactgatggc gttcgtggta ctgcctatcg
       61 cctggcaaca gggcatctac ttaatccgcg atatgccggg gatgctcaat aagctttctg
      121 actttgccgc cacgttgccg cgccgctatc cggcgttaat ggacgcgggc attattgatg
      181 caatggccga aaatatgcgc agtcggatgc tgaccatggg cgattcggtg gtgaaaattt
      241 ccctcgcctc gctggtcggt ttgctgacca tagccgtcta tctggtgctg gtgccattga
      301 tggtcttctt cctgctgaaa gacaaagagc agatgctgaa cgccgttcgc cgggtgctgc
      361 cgcgtaaccg tgggctggca ggacaggtgt ggaaggagat gaatcaacaa atcaccaact
      421 atatccgcgg caaagtgctg gagatgatcg tggtggggat cgccacctgg ctggggttct
      481 tgctcttcgg gctgaactat tcgctgctgc tggcagtgct ggtcggcttc tcggttctta
      541 ttccgtacat tggcgcattt gtggtgacca ttccggtggt tggcgtggcg ctattccagt
      601 ttggcgcagg tactgaattc tggagctgct tcgcggtgta tctgattatt caggcgctgg
      661 acggcaacct gctagtaccg gtgttgttct ccgaagcggt taacctgcat ccgctggtga
      721 ttattttatc ggtggtgatc ttcggtggtt tgtggggatt ctggggcgta ttcttcgcca
      781 ttccattggc gacgctgatc aaagccgtga tccacgcctg gcccgatggg caaatcgcgc
      841 aagaataatc ttcagcccga cgaactgttt cgccgggctg atttttcagg attcctgacg
      901 taaataaata gcccgataac aaatactcac cagcactgca ccgccgataa tattacccag
      961 catcaccggg agcagattgg cggtaataaa atggctgacc gtcagtgccg gaaaattgtc
     1021 tgcgctactg tgcgccagtt gccagaagga ggtaggggcg aaatggcgaa tggcaatggc
     1081 gaacggaatc acaaacaaat tggcgataca gtgctcaaag ccactggcga caaacagggt
     1141 gatgggcaaa atcatggcga cgattttgtc gcataacgaa cgtccgcaat aactcatcca
     1201 cagcgccagg caaaccatca gattgcacat aatgccgagg ctgacagatt cagtaaatgt
     1261 atgatgcatt ttgccctcgg cgcagtgtaa aatcgcgact ccccacatcg cgttttcact
     1321 catcaccagc ccggaaaacc agattaacaa actgaaacag gcaatacctg ccagattacc
     1381 gcaggccacc agaagtgcgt taatcagcca agttcgccaa ctaataacgc cccgactttt
     1441 tgccattacc gtcattaccg acgaggtgaa caggctggtg ccgcaaaccg ccagcaaaat
     1501 aaagccaagt gtaaagcaaa ggccgcccac cagatgggtt aatgcctgtg aagacggggc
     1561 atcggcgata acggaaaggt aaaaagtaaa tccgatcgcc ataaacacgc cagcggacat
     1621 cgccattaaa aagaccgaca ctttactgcg ggcaatttta tgggcggcaa tccgttcagc
     1681 gattgccgct tttctggcgc tcaactgcaa gtcgaaagag agtttgtttc tcatgcatac
     1741 tcctgcaaaa gcagattaca acacctgcga accgagattc ccagacgctg cattcgtgac
     1801 agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc cacggggccc ggcaacaatg
     1861 ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt cttcttcgtc attctctggc
     1921 gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg ctgaatcttc atttagcgtc
     1981 ggcagtaaac ggctttgtcg gacattaaga tgtaaattca gactgttacc gcgagtcagc
     2041 agtaccgctc gctcaatcac gttttccagc tcgcgcacgt tgcccggcca gtcccacgac
     2101 atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg aacgattcat atggcgcgcc
     2161 attttttgcg tgaaatgctt tgctaaaaga gggatatctt ccggacggtc gcgcagcgga
     2221 ggcaattcca gtgggaagac attcaggcga taaaagagat cgctgcgaaa ctggcgatct
     2281 tcaaccattt gccacaaatc acggttggtg gcggcaatga ctctgacatt taccgggatc
     2341 gttctactcc cgccgagacg ctcaatctcc cgttcctgca atacgcgcag cagtttaggc
     2401 tgaagttcta acggcagatc gccaatttca tcgagaaaca acgtgccgcc atcggcaatt
     2461 tcaaaacgac cacgatgggt attaatcgca ccagtaaacg cccctttgtc atgaccgaat
     2521 aactcacttt ccagaagact ggcggggatg gcagtgcagt tgattttgac cagcggcttg
     2581 tcgtgtcgcg ggctaagttg atggatcgct ctggcgatca cctctttgcc ggttccggtt
     2641 tcaccgcaaa tcaacaccgt actgtcgctc ttcgccacaa tatctacctg ctggagcagc
     2701 tcttccattg cctggctttg atagataatg tcaccgacgc ccagattcga aaggagctgc
     2761 tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca aatcggtcat tctacgccag
     2821 gcatcggcat tgccaacggc aatagcgatg cgatcggcta tgtgttgtag tagctggcag
     2881 ttttcctcac taaagagagt ggaagaggta tgcgccagca acaatgcacc cggtgtatgg
     2941 ttgccaaagg taagcggtat aaggagcgca gattcgcagc cgttagatgc aagtaagagt
     3001 aataacgggt cgcgttgcca gagaaacaga tcatctgcac ggtgcgtcaa ggtcggttgt
     3061 cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag gcatattgcg tggcagacac
     3121 gcacaatgtg aggcagaaag atcgctgcac cacaggctga acttctcgtt ctttcgatga
     3181 tcgcccagta ccatactgac tgaagccaga ccgaaaaaat gatggatctc acgagcgacg
     3241 tcagcgatca gatcgtcgag atcaagatgt gacagcactg cattagtgat atccaccagt
     3301 acccgatgat gatcgcgatc tttctccagc aaacgcagcg ccacctgctg ttcttcatca
     3361 ttgagccatg attgcagaac cggggtgata atgcgagcca gccagttaaa cagtgtgcaa
     3421 tcctggggag taaaacggct gccgtgggta cgaataaagc gtagctccgc cattccacta
     3481 ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct cgccctgata ctctgttgtt
     3541 gccgtcacat cgctgccatc atcatggtag cggtaataga agttgctttt gagaacatgc
     3601 agttcaataa gttgtaccgg gctgaacggc ggcggcagcg ggatgaaggc gcgtaataaa
     3661 gacgccttgc cgtgtttctg cgctaaccgc tccgcgagca ttccgtttac cgcttcaatt
     3721 gttattcctt gcggcggggc aaacatcgct tcgtctgaca tagccatcaa cagtccttat
     3781 taatgagaaa tccttctcac gcaatttttc tggccatcag atagatagcc ggttccagca
     3841 caatttcatc caacagatga atgaattgaa tgctacgcgc ttgtcacaca gacgtcgcgt
     3901 catctgccag tggcgcgaat gcccgggtca acagggcggt gtggctggcg tcaataacaa
     3961 tctcaccaaa agtcatcacc cccagcatct tacgtcgggc ttgctcctct tcgaccagtg
     4021 caagccaatc ttcgtattcc gtcagtgggc agcggaaggc gacattcaga caatcaatca
     4081 cgccaacgtg atgaccgata gccagcgagt aatacattac ctgagagctg tgttccggca
     4141 tctcgtcgct actggcgaca aacttttttc gcagcgtcca gaaaacaatt tccccgcact
     4201 cttcagtcat gtaacccccg gagtacgcga aagagttgct gaacgatact attgagacgt
     4261 ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc ctgtaggatc gtcgcttaaa
     4321 tggcgcagga gccgatcgca aatttctcgc ccctgacgat agcccgccag ccgccgcgct
     4381 tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg gccacaacgg ttgcatagtc
     4441 accccggtgg gatcgcgata atccaccgcg tgaatcttct gttgcagcaa accgagcgcc
     4501 acggcgaaac cgtgaatggt ggcggccggt gttggcgggc agccggggat ccaaacatca
     4561 atggggacaa tggtgtcgct gccgccccag acgctgtaga gatcgtggaa aataccgccg
     4621 ccgacaccgc acgcgccgta ggaaacacag attttatgat cgggggcaga ctcatacgcc
     4681 cgaagtgcag gcatacgcat cgcccgggtg actgcgccag taaataacaa aatatcggcg
     4741 tgacgcggtg atgaaacaac cttaatgcca aaacgttctg cgtcgaatac gggggtgatg
     4801 gcagcaaaaa tttcgatttc acaggcgttg cagccgccgc aatcgatgcg ataaacgtaa
     4861 gccgagcgac ggatatcctg tagcaaatgc cgcttcatct tttgcgtttg ctcgtccagc
     4921 gtgatgggct ggctgacata ttgcgtaagc actggactca tagctgctcc ttagccacca
     4981 gcggtacatc tgtatcgtcg ttgatcagcg tcgcgcgttg tttgcactcc gggcagacgc
     5041 tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt ttgttgctgt gccaacaatt
     5101 cagcagccag tgcgacggtt ttttgcgggg caaacgggcg ttcgcaacgg ctgcaacgtt
     5161 gtagatggaa cgtcgcgtgg gtatagagat cggctttatg ggtgacggtc agttcaaagt
     5221 tattggtaag ctggatggct ctggtcgggc acacttcttc acaacgtccg cagtaaatac
     5281 aacgccccag atagagctgc caggtgcgcg aattttgctg gtcgtcggtc tggatagtca
     5341 gcgcatttgc cggacaagca caggcacagg cgcaggcacc gcaggcaata cattggctgg
     5401 gcatcaggtc cggtttcccg cgaaagcctg ggctgacctc cagtggcgcg aagggatatt
     5461 tcaccgtcgt ggttccggcg cgcataatag ttttcagtaa cttcagcatc ttctgtcctt
     5521 acttcagcgg cgaacggtta cgatcaatgc cgtagcgttc gatctcttta tacggcacgg
     5581 tttttgactg gcgcttgcgc acatctacca gcgtcacacg gtcggtacag gagtagcagg
     5641 gatcaaggct accgataatc agcggtgcgt cagaaacggt attgccgcgc agcatgtaac
     5701 gcaacaccgg ccagttggcg taggtggcgg cacgacagcg ccagcggaac aatttttggt
     5761 tatcaccgag catgctccag tgcacgtctt caccgcgtgg cgcttcaaca aagcccagcg
     5821 cgaatgcgtg aggtttatag ctaaagcctt cggtcagcag tggggtatcc ggcatgttgt
     5881 cgagggcaaa ttccagcatt gccagcgaat caaacgtctc tttgacacgg accatcacgc
     5941 gggagaaaac atcgccgccg gtaaaggtaa acagtgtttt tggaatattg ccgtagtcgg
     6001 cgtaggggtg atcaaagcgc aaatcacggg caaaaccact gcgcggatca gcggccctac
     6061 agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag tgcgttgttc
     6121 catattcggc gtagcaagca gcatctctac cagctccgac acgtcggcgc gcatctcgcg
     6181 cactagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa caccaccaat
     6241 cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccatcg tcatcgactt
     6301 ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaaa ccaacaaaat ggcaggagag
     6361 gccaaggttg agcaaatgac tgtgtagccg ctcgacttcc agcagaatcg agcgaatagt
     6421 atgtgctcgt tgcggcacct caatccccag tgcattttca accgagttgg tataggccac
     6481 actgtgggca aaaccgcaaa tcccacacac gcggtccgat aagaaggtca cttcgttgta
     6541 gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca ggcggtaatc
     6601 agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt cataggaggt
     6661 gatatgcagc gggccgacag ggatcacccg cgcatcgctg ttgccctcat tgataaacgc
     6721 atacgtttcg gaatctgtcg tcggttcagg gcgcagtcga taatccatcg catctttgcg
     6781 cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct gatccggcag
     6841 gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga ccgcggcagg
     6901 gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg ccttcaccac
     6961 gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt aatgaccgtt
     7021 aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt gataataaag
     7081 ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct gttcaggcgt
     7141 ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg cgagaatcgc
     7201 ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca gatgcggtgc
     7261 ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc gtccctgaga
     7321 ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata tgcgttccca
     7381 tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag ccgagatcac
     7441 cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgcagccatc cgtaccaggc
     7501 ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg tgattacgtg
     7561 ccagtcctgc ggtaacggtc ataaattcgc taagaaaaat gttgaagggc ggcatccctg
     7621 ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt ttgagtatcc
     7681 cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg gaaccgcaga
     7741 acagcagcgt ttttgccaga ctgtggttta aggtgtgcag cagcgcggca aaaattccca
     7801 gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg ctggagtacg
     7861 ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg gcaaccgaca
     7921 acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggcc tggcaaataa
     7981 tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg gagagcaggg
     8041 cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc atgggaaata
     8101 gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc attaatgttg
     8161 ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt tctgcctgcg
     8221 gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca aaagcgacac
     8281 caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc tgaccgtaaa
     8341 tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac atcacaatca
     8401 ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag aaaccgtagt
     8461 aatcgcacag tgtaacgggt gaaagctcgc cgcgtgccac ttcatgacgc atgtagccaa
     8521 tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac aaaccgccca
     8581 gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca gcggcagttt
     8641 ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt aatacagtca
     8701 ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc gaaaaaagca
     8761 gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta tcccttcagc
     8821 gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct tgccagtaac
     8881 accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac cagttccggt
     8941 gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag gcagtaacca
     9001 aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag cagaaaatga
     9061 cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt cacgggctga
     9121 acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat tgccggaccg
     9181 aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt catgattaac
     9241 ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag ttgctctgcg
     9301 gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg gcaggcgtaa
     9361 aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt cagcatcatc
     9421 agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct tactgcaacc
     9481 aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc aacaccaggc
     9541 gcattgaccc tagcagcggt gcggcatcgg cgacggcctc gctcggtttg cccggcacga
     9601 cgcgaccgaa ccagcgaata aaccaggcga aactggcgac cgattcaatc atcagcagaa
     9661 tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccggcg gcaaacagcg
     9721 ggaatttact gaagaagccg ttgaacggcg gcacaccggt aattgccagc gctgccacgc
     9781 agaaacccac gcctggcaac ggcagggtgt gcaatacgcc acgcagacgc ggcaacaagc
     9841 gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt ttagcgaacg
     9901 cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg aagatggaga
     9961 gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc agcaaccgct
    10021 tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc agtgccatga
    10081 ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg gcgcgggcaa
    10141 aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcgctg atcggtgttg
    10201 gcgcttccat tgcgtccggt agccacgctt gcatcggtag ctgggccgat ttcccccacg
    10261 cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccatgtaac tcgctcatcg
    10321 cgctaagcac aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca
    10381 acgaaccgat atgggtgata agcaacgctt ttagtgctga acgctgcgct ttatcgctct
    10441 ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac aacaactgac
    10501 cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc agcaaaaatg
    10561 cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc agataacccg
    10621 tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc agcgtactca
    10681 cgcgatcaat gaccaggcca aacaatgcca catcccccac gttgaccaac gtcaacgtga
    10741 cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac agcgtggtca
    10801 gcgcggcgaa caaaaccccc cattcggcgg cccgacgttg tggcgaaaac gaaacgacca
    10861 gtgcgccgat aaaaggcagc attaacgtcg tcagagcaag attttccata ttccgtcagt
    10921 gctccttaca gaccggttaa ccagaagacc catgcaagcg cagcaaggct gaagccaagc
    10981 caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag
    11041 gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag
    11101 gcgagagaaa gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc
    11161 atcacgacct gtttcagccc cagcccccat ttcgctagcg ccagcccggc accggaatat
    11221 tcggtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt
    11281 cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc
    11341 gagttccagc ccatcgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc
    11401 agcgccaata ccagcagtga gagaataagc attggttcga ccagaatgcc gagcgtcaac
    11461 tcgcgactgg caccgactcc cgcaaacgga cttccggtat ccagcccgga aagagcaaag
    11521 aaaaaacgaa acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa
    11581 ggggaaacgg taataaatag tggtaaggcc atcgccagca ccagcatgct gctgattaat
    11641 acccacggca tcaggcggaa catcagacct gaagatgtcg gcgcaacttc ctggcgttta
    11701 aacagtttgt ggatatcgcg ataatcctgc cagatccccg gcccgcggcg ggagtgcata
    11761 cgcgcgcgta tctgccggga aatacccgta aaaagtgggg ttagcatcag cagaatcacg
    11821 gcctgtgcta acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct catggtgatt
    11881 tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcgaccacat acaggcaata
    11941 gagacgaaag tcgccgcttt gcagatgctg tatttctttg accagccgtt gggtggcgct
    12001 cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt
    12061 gacgtgcgca agacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa
    12121 taacgcagag aagaccacac gcagcggctg catcacgcca ttgcctgacg gagccatcgc
    12181 gttttctcag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg
    12241 cgaacgacac cacagccagt aaaggccagg cagcaaaggc atcgccagta gtaacagcaa
    12301 cagtaatgaa ggagtgaccc gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc
    12361 gacagtgacg ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag
    12421 ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat
    12481 catcgtattt ggcacttcct gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa
    12541 agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga ctgccagcat
    12601 taccagagca ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa
    12661 cagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa tggctgatat
    12721 cgcgaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc
    12781 cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcgcccgcgc cgagaaatag
    12841 cagccctttg aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaaccac
    12901 ggtgagcagc gggtcgtgca gtgacagacc gtccatcgcc acaccgactg ccagcaaaat
    12961 aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag
    13021 cgcatatagc acgcccagga gcgcggagat tgcgccgatc gccatcacca gaatgcccca
    13081 caacagaggc aaacccgttt gcgccagcag atccatcgct actttcagga tgccgaaaat
    13141 accgattttg accattacgc cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg
    13201 agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcggcaa aaccgaaaaa
    13261 ggccagcaga aacaccgccg acgccagccc cggagaaagt gaaagcgtgc gaaactggca
    13321 aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcataat cagcaccgat
    13381 ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt atcctgcctg
    13441 gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat gaaccaaaaa
    13501 gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaagattcat aaagaagccc
    13561 atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata gagcgaacaa
    13621 accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc cattcgcagg
    13681 gttaaatcgg caaagtcaaa aggggtggca aaatgggcga ctaaagttac accagcatgt
    13741 aattgcgtaa ttccgctgat gatgccaatc acgccgcaca ccagcgatgt gatgccggaa
    13801 agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact ggcaaacaga
    13861 tagaggatca gcgaccaggt taataattgc agggcatcca ttagtgttgc tcctgagtga
    13921 gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc tgtttttctt
    13981 tcatctgacg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc gggcaagcgc
    14041 gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc gcgacggtct
    14101 ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct tcgccgtctt
    14161 tgagtgagcc ttcagcctga aaaacatatt gcgcatgggc attcaccgga cggcttcctg
    14221 aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca atacagaggc
    14281 tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg cagacctgca
    14341 ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgattgtt gacgtcttcg
    14401 ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc gaacaggcag
    14461 cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag cggttcatgt
    14521 cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga tgccattctg
    14581 attaactcaa tctattcaat aagttatgta ttaactggat tctgaacggt acaaaaatga
    14641 tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg aaagcgagaa
    14701 tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag cacggaatgg
    14761 agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg tgattgctgg
    14821 ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg aagctgatgc
    14881 gatgaatacc atcgtaggtt ttgcccatga aggacttttc accccagacg ccgaattgtt
    14941 cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct ttttccgcaa
    15001 aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg cccgcttttt
    15061 tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg
    15121 ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt
    15181 ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc agtggattca
    15241 ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa tactgccttg
    15301 tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg cgtccgcaga
    15361 tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat tttcagcagg
    15421 ttgtgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa
    15481 gcgttcaatt aaatgcgggg agtctgcaac atcgacctga acccagacag atgctggcat
    15541 tggcggacgc ggacgcgccg tcgtgctctt catcacgatt aaaagatcca gttcggcacc
    15601 tttcaacggt aacgttgatt caatcagagt gatggcattc catgaaccgg aaagcagcat
    15661 gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat tacagccgca
    15721 actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac ccaacgcagt
    15781 gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa ggggtgagtt
    15841 gttcttaagg aaagcataaa aaaatcatgc atacaacaat cagaacggtt ctgtctgctt
    15901 gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag gatggcccat
    15961 gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta atgtctgtcg
    16021 ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc
    16081 tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg atgtggtgat
    16141 gatgacgctg gatctggctg atgggcgcat tccggtaatt gccgggaccg gcgctaacgc
    16201 tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg tcggctgcct
    16261 gacggtaact ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc
    16321 catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc gtactggctg
    16381 cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa
    16441 agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt
    16501 tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattgg gcggtcatgg
    16561 ggttatttcc gttacggcta acgtcgcagc gtgtgatatg gcccagatgt gcaaactggc
    16621 agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc cattacacaa
    16681 caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg aactgggtct
    16741 tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg gtcgtgagac
    16801 ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat ttgatggctt
    16861 actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg
    16921 ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa gcctacctgg
    16981 aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg gtgacctccg
    17041 gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg ctggacattc
    17101 gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc acgggcgata
    17161 ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag gtggttagcg
    17221 tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag acactgacca
    17281 ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt cgttatcaaa
    17341 tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgctg aacctggaac
    17401 aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg gagatgatga
    17461 acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct gcgcaaaatc
    17521 gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta ccaatgctgg
    17581 tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg gaaaaagtgg
    17641 gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact tataagccgc
    17701 tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca tccggtgact
    17761 ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc gatccgaaag
    17821 gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag gctgcgttta
    17881 gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatataa tacgcaaacg
    17941 tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg tttgtcggac
    18001 gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac cagacagcaa
    18061 aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc aagctgagtt
    18121 gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt tggtgctcga
    18181 attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg
    18241 tatggtgaac aacaagttca actacttcat tatgagcaaa ctggctgaag cgggtatccc
    18301 gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc tggatatggt
    18361 gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac gtcttggaat
    18421 cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa acgatgccat
    18481 gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga gcaaagagaa
    18541 cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa aactgttcga
    18601 tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca aaggtgaagt
    18661 ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag aaacactgga
    18721 gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag cctatgaagc
    18781 cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat cttgccgtgc
    18841 agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca ttcctccgcc
    18901 atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca tttctataat
    18961 gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga caatgttgaa
    19021 gacaggcgca acagctctgg cggtccgtca atgggcggtc ccggtttccg tctgccgagc
    19081 ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta tggcgttgat
    19141 ttaaccgggt tgatgaccgg gcagccggtt tctcaacaac aatcaacgcg gtcaattagc
    19201 cctaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac ggaagacacc
    19261 tggggacaac agttcgaaaa gatgggtaag acctatcagc aaccgaagct ggtcatgtac
    19321 cgtggaatga cgcgcaccgg ctgcggggcg ggccagtcca ttatggggcc gttctattgc
    19381 ccggcggatg gcacagttta tatcgatctc tccttctatg atgacatgaa agacaaactt
    19441 ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg tcatcatgtg
    19501 cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc gacgcaggcg
    19561 gaagtaaacc acttatctgt gcgtatggaa ctccaggccg actgttttgc cggtgtctgg
    19621 gggcatagta tgcagcagca aggcgttctg gaaactggcg atctggaaga ggcgctgaat
    19681 gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg agtagtacca
    19741 gacagtttca cacatggcac ttctcagcaa cgctacagct ggtttaaacg tggtttcgac
    19801 agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaattttc ggggcaggga
    19861 tggctgaact gactgcgctt tacacattaa cagcgcaaat gaaacgtgaa gggatctgcc
    19921 gcttgctggt gttgagcggg gaagagggtt ggtgttttga tcatgctctt aagttgcgtg
    19981 atgccttacc tggcgactgg ctgtggattt cgccgcagct agatgctgaa aaccactgtt
    20041 caccctcggc gctacaaact ttacttgggc gcgagttccg gcatgcggta ttcgacgccc
    20101 gccacggctt tgatgccgct gcctttgcag cacttagcgg aacgttgaaa gcgggaagct
    20161 ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat gccgactcgc
    20221 tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag catctcaaac
    20281 gcgtacttac ggcggataac gacgctatcc tctggcggca aaaccagccg ttctcgttgg
    20341 cgcattttac tccccgtact gactggcacc ccgcgaccgg cgcaccacaa ccagaacaac
    20401 agcaactctt acagcagcta ctgaccatgc cgccgggcgt ggcagcggta acggctgcgc
    20461 gtgggcgcgg taagtcggcg ttggcagggc aactcatttc tcgtattgcg ggcagtgcga
    20521 ttgtcaccgc gcccgcaaaa gcggcaacgg atgtactggc acaatttgcg ggcgagaagt
    20581 ttcgctttat tgcaccggat gccttgttag ccagcgatg
//
LOCUS       DADLSB010000062        20528 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.062, whole genome shotgun sequence.
ACCESSION   DADLSB010000062 DADLSB010000000
VERSION     DADLSB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.062"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..686)
                     /locus_tag="JN331_002193"
     CDS             complement(<1..686)
                     /locus_tag="JN331_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7260332.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARG"
     gene            860..1573
                     /locus_tag="JN331_002194"
     CDS             860..1573
                     /locus_tag="JN331_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7260333.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            1605..2363
                     /locus_tag="JN331_002195"
     CDS             1605..2363
                     /locus_tag="JN331_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260334.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            2409..3758
                     /locus_tag="JN331_002196"
     CDS             2409..3758
                     /locus_tag="JN331_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY7260335.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3758..4582
                     /locus_tag="JN331_002197"
     CDS             3758..4582
                     /locus_tag="JN331_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260336.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            4594..5153
                     /locus_tag="JN331_002198"
                     /pseudo
     CDS             4594..5153
                     /locus_tag="JN331_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
     gene            5184..6254
                     /gene="lptF"
                     /locus_tag="JN331_002199"
     CDS             5184..6254
                     /gene="lptF"
                     /locus_tag="JN331_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7260337.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYNVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            6251..7242
                     /locus_tag="JN331_002200"
                     /pseudo
     CDS             6251..7242
                     /locus_tag="JN331_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
     gene            complement(7278..8450)
                     /locus_tag="JN331_002201"
     CDS             complement(7278..8450)
                     /locus_tag="JN331_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7260338.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDVIIQAFATLKQ"
     gene            complement(8450..8698)
                     /locus_tag="JN331_002202"
     CDS             complement(8450..8698)
                     /locus_tag="JN331_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7260339.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8730..9626)
                     /locus_tag="JN331_002203"
     CDS             complement(8730..9626)
                     /locus_tag="JN331_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY7260340.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            complement(9641..11328)
                     /locus_tag="JN331_002204"
                     /pseudo
     CDS             complement(9641..11328)
                     /locus_tag="JN331_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            11732..12874
                     /locus_tag="JN331_002205"
     CDS             11732..12874
                     /locus_tag="JN331_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7260341.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQDFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(12998..13768)
                     /gene="glcC"
                     /locus_tag="JN331_002206"
     CDS             complement(12998..13768)
                     /gene="glcC"
                     /locus_tag="JN331_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAY7260342.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            14019..15518
                     /gene="glcD"
                     /locus_tag="JN331_002207"
     CDS             14019..15518
                     /gene="glcD"
                     /locus_tag="JN331_002207"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAY7260343.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVDVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            15518..16570
                     /gene="glcE"
                     /locus_tag="JN331_002208"
     CDS             15518..16570
                     /gene="glcE"
                     /locus_tag="JN331_002208"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY7260344.1"
                     /translation="MLRECDYSQALLEQVNQAISDKMPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLLGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            16581..17804
                     /gene="glcF"
                     /locus_tag="JN331_002209"
     CDS             16581..17804
                     /gene="glcF"
                     /locus_tag="JN331_002209"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAY7260345.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTFKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            17809..18213
                     /locus_tag="JN331_002210"
     CDS             17809..18213
                     /locus_tag="JN331_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAY7260346.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            18235..20406
                     /gene="glcB"
                     /locus_tag="JN331_002211"
     CDS             18235..20406
                     /gene="glcB"
                     /locus_tag="JN331_002211"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY7260347.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
BASE COUNT         4967 a         5109 c         5522 g         4930 t
ORIGIN      
        1 taaccacgcg cggtcataaa atccgtaatc gctttttctg catcaaccaa cacctgttcc
       61 agtggctgat tggcatcgat atcaaccagt tgtgcaccac caaaggttaa ctgtggcgtt
      121 atggcaatct tcctcgccag cgattcccgt ttatggtcgg gtttacgtgc acaggcaact
      181 tcaaggtcaa cattgagttt gatgaccaga tcaggcttat ggctcgccat ccagtgaaac
      241 gctttacgtt cctggcttgc cagccatgag acaaaacgac caccttcaac gttaggtggg
      301 aacaccgtac catcgtaagc gccaggaatt tggtcctgag gataacggtc ggttagaaca
      361 attaacccgc gacgacgaca ggcaagcata tgacgaaagc gcagtaagcg acgggcgaca
      421 aacgctgtaa ttaccagcgc cggaactggt ccaggcaatt tttttgctgt tttcacctga
      481 tttcgttcaa ttgttttatg taaggatttt cccatcaaag gtaattttgt cactgcacga
      541 ccgacatttc cagcctgttt tcctaaatga actctttcgg cagcaccata tttttcgaca
      601 acggtaataa gatgttcaca caccgttgac ttgcctgaac catcgctacc aataacggca
      661 attaatggag gtgttattga ctgcatgaat atatccttta atttaaatat ccattaaaaa
      721 tatttatttg gttaatatgt ttttatgaaa gcgtaattca ggtcaatgtc acaattaacc
      781 attgtcacaa taaggttgaa cggatattct gagcacggac cacgtaatat tcaatacatt
      841 atttactgcc gggtttttcg tgaatcaacg taatatgtca ataattaatt ccacgccagt
      901 gcgtgttatt gccattgtag gatgtgatgg ttcaggtaaa tcgaccctca cggcaagcct
      961 ggtaaatgaa ctggcagcaa gaatgccaac agaacacatt tatctcgggc aatcgtccgg
     1021 gcgaattggc gaatggattt cacagctccc tgttattggc gcaccttgtg ggtgttatct
     1081 gcgaagtaaa gcggcacatg tacacgaaaa gccctcaaca ccgcctggca atattactgc
     1141 gctggttatc tatctgcttt cctgctggcg ggcgtacaag tttcgcaaaa tgttgtgtaa
     1201 aagccagcaa ggctatctgc tcatcaccga ccgctacccg caggttgaag tgccgggttt
     1261 tcgctttgat ggcccgcaat tggcaaaaac cacgggcggt aacggttgga taaaaatgtt
     1321 aaggcagcgc gagttgaagc tgtaccaatg gatggcgtct tatttacccg tattgttgat
     1381 tcgtcttggc attgatgaac aaaccgcgtt tgcgcgtaaa cctgaccacc aactggcagc
     1441 gttacaggaa aaaatcgccg ttacgccgca actgacattc aatggcgcca agatccttga
     1501 actggatggt cggcaaccag ccgatgaaat tatgcaagcg tcactacgcg caattcacgc
     1561 cgcgctttcc tgatcaccaa aatggatagg ggatgattta acgaatggat gcactacaaa
     1621 cacaaactgt taatagtaca accgcgccgc agcccaacta cattccgggg ctgattgcgg
     1681 tggtcgggtg tgatggcacc ggtaaatcca cgctgaccac cgacctggtg aaatcgctgc
     1741 aacaacactg gcaaaccgag cgccgctatc tggggctgct ctccggcgaa gacggcgaca
     1801 aaatcaaacg attgccgttg gttggcgtct ggctggaacg gcgactggcg gccaaatcct
     1861 cgaaaaccca aagcatgaaa accaaatctc cggcgctatg ggcggcggtg attatgtact
     1921 gcttctcgct gcgaagaatg gcgaatctac gcaaggttca gcgactggcg caaagtggcg
     1981 ttctggtggt cagcgatcgc ttcccgcagg ctgaaatttc gggcttttat tatgacggac
     2041 cggggatcgg cgtcgaacgt gcgaccggga aaatcagcat gtttctggcg cagcgcgaac
     2101 ggcgtttata ccaaaaaatg gcgcaatacc gtcctgaatt aatcattcgt ttggggattg
     2161 atattgatac tgccatctcc cgcaagcctg accatgagta tgccgagctg caggacaaaa
     2221 tcggcgtcat gtcgacgatt ggctataacg gcacaaaaat tcttgagatt gattccagag
     2281 cgccctacag cgaagtgctg gaacaggcac agaaggcggt ttctctggtt gccatcgttt
     2341 ctgaccgccg aagtttaact taaggctggc gggatatttt ttactatcaa cgataaggtc
     2401 aggctgaatt ggcgggtttt aacatcaaac attggtttgc agatggcgcg tttcgcacca
     2461 ttattcgcaa tagcgcctgg ttaggctcca gtaatgtcga gagcgccttg ctggggctgt
     2521 tggcgctctc gtgtgccggt aaagggatga cgcccgccat gtttggcgta ctggtgattg
     2581 tgcaatcgta cgccaagtcg atcagcgatt ttattaagtt tcagacatgg cagctggtgg
     2641 ttcagtacgg aacgccagcc ttgaccaaca ataatccgca gcaatttcgc aatgtcgtct
     2701 cattttcctt ctcgctggat atcgtcagcg gcgcggtggc gattgtcggc ggcattgcct
     2761 tactgccatt cctttcccat tcattaggtc tggatgacca aagcttttgg ctggcggcgc
     2821 tgtattgcac gctcattcct tcaatggctt cctccacgcc gaccggcatt ctgcgtgcgg
     2881 tagatcgctt tgatttgatt gctgtacagc aggcgacgaa accttttctg cgcgcagcgg
     2941 ggagcgtcgc agcctggtat tttgactttg gttttgcggg ttttgttatt gcctggtacg
     3001 tgtcgaatct ggttggcggc accatgtact ggtggtttgc cgcgcgcgaa ttacgccgta
     3061 gaaatatcca taacgccttc aaattgaatc tgtttgagtc tgcccgacac attaaaggcg
     3121 cgtggagttt tgtctggtca accaacattg cccactccat ctggtcggcg cgtaactcgt
     3181 gcagcacagt gttggtgggg atcgtgttag gacccgctgc cgccgggtta tttaaaatcg
     3241 ccatgacatt cttcgacgcc gccggaacgc cagcgggttt gctgggtaaa agtttttacc
     3301 cagaggtgat gcgtttagat ccgcgcacca ccagaccgtg gttgttaggt gtgaagtccg
     3361 gtttactggc gggcggaatc ggtattctgg tggcgctcgc ggtgttgatc gtcggcaagc
     3421 cgctcatttc gctggtgttt ggcgttaagt atctcgaagc gtatgacctg attcaggtga
     3481 tgctgggcgc aattgtgatt tcgatgctgg gcttcccgca ggagtcattg ctattgatgg
     3541 cgggaaaaca gcgcgctttt ctcgtggcgc aaaccatcgc ctcaatcggc tacatcgtat
     3601 tgctgttcat gttctgtcat ctgtttggtg tgctgggagc agcgtttgct tacttcggtg
     3661 ggcaatgtct ggacgtggcg ctctcgttaa ttccaacctt aaaggcattt tttcagcgcc
     3721 attccttgct ttataacgca gccggagaga aatcctgatg atgagaaaat atttcccgct
     3781 tgaggcgagc gagcgtctat ttgttgcgat tgaagaagat gatgtcgttg atgcacaggt
     3841 tagtttgccg ccgactattg cactaagttg taccaccgaa atcattcatg ataattacgc
     3901 gttatgcctg caattctggc tcaatggcgt tgatcgtcag gaattactgc gtttagttcg
     3961 caaacaagcc aaaggcgatg agctgactgc tgatgagcgc aaacaattta aatacatgcg
     4021 agcgcgctac aagcacctgc gttttgcgca acggctgtat ttaaagaagc atcaggccgg
     4081 atttttattc ggcaaaacga ccgtttttct ggggcgtttt caggacggct ttcgtaacgg
     4141 taagaaaaat attgtgtcgt attacggcaa cctgttacgg atttatttaa gctctccggt
     4201 ctggtcgttg gtgaactatt cattacgcca tagccagtta gaaagcgtaa gcagttttat
     4261 tgcctatcgg caaaagcaaa tgcataccct gaaagagatt attgccaaac cgcgactaac
     4321 cggcagggaa tttcatgatg tgcgaaaaat catcagccag caggtttctt attacgacac
     4381 cttaagatcg cttgatcctg aaaataaaga ggcgttacag atttcccgtt tcctcgccgc
     4441 cattaatggc ctgatgggag ataaacacga cgacatggtg gcggatgata tggaaaaccg
     4501 ccagtcgtat gatgcaccgt tggcgttgga tagcgatatt cgtcagcgct tagagctgct
     4561 gatttcgcgt tttccgctat agccggagca atgatgtcat ctcgtttgat tattgcgttg
     4621 attatcatgc tcttagcccc gagtgtgcag gcacacaatt tcgtcactgg caaaacggtt
     4681 acccctgttt atatccagga aggcggcgag ctgcttctga atagcgacga cgaaattcac
     4741 taccaaaagt ggaacagcac acaattagcg ggaaaagtcc gcattattca gtatatcgcc
     4801 gggcgtaagt cggcgaagaa aaagaactca ctgttaatta aagcggttga agcggcaaac
     4861 tttccgcagg atcgttttca gcccaccacc attgtgaaca ccgatgatgc catctttggt
     4921 actggctatt ttgttgttgg caaaatcgaa aaaaataaac gcgctatccg tgggcgcaat
     4981 tcgttattga cggcaatgga cagggacgcg ttgcctggcg cttgccggag cagagttcaa
     5041 ccattctggt gctgaataaa gccgggcaaa ttcagtgggc aaaggacggc agcctgacgc
     5101 cggaagaggt agatcatgtc attgcgttgg cgcagaaact gatcaatgaa taaaaaactc
     5161 aaaggattga gggcgttagt gagatgaagc tggttgaaca ctacatcatg cgtggtacgc
     5221 gccgtctggt gctgattatc gtcggttttc tgatctttat tttcgccagc tactccgcac
     5281 agcgttatct gaccgaagcg gcaaatggga cattagctct tgatgttgtg ctggatatcg
     5341 ttttctacaa cgtactgatt gcactggaga tgttgttacc tgttggtctg tatgtgtcag
     5401 ttggcgtgac gctagggcag atgtataccg actcggaaat taccgctatc tctgcggcgg
     5461 ggggcagtcc gggacgcttg tacaaagccg ttctttatct ggcgataccg ctaagtattt
     5521 ttgtcaccct tctgtcgatg tatggtcgtc cgtgggctta tgcgcagatt tatcaactgg
     5581 agcagcagtc gcagtcggag ctggatgttc gccagttgcg ggcaaagaaa tttaacacta
     5641 acgataacgg acgaatgatc ctttcgcaga cggttgatca ggataataat cgcctgactg
     5701 acgcgctgat ttatacttct actgccaatc gaacccgtat tttccgcgcc cgttcggttg
     5761 atgtggttga cccatcaccc gagaaaccga ccgttatgtt gcacaacggc actgcctatc
     5821 ttctcgatca tcaggggcgt gacgacaacg aacagatcta ccgtaatctg caattacatc
     5881 tgaatccgct ggatcaaagc ccgaacgtca aacgcaaagc gaaatcggtc acggagctgg
     5941 cgcgctccgc ctttcctgcc gatcatgccg aactgcaatg gcgacaaagc cgtggcctga
     6001 ccgcattgtt gatggcgctg ctggccattt cattaagtcg ggtaaaaccg cggcaagggc
     6061 gattttcaac gttattgcca ctgacgttgc tgtttattgc cattttttat ggcggcgacg
     6121 tctgccgtac actggtggct aacggcgcga ttcccctcat tcctggtttg tggttagtgc
     6181 ccggactcat gctaatgggc ctgctgatac tggtcgcacg tgacttctct ttgctgcaga
     6241 aattttcccg atgaatgttt tcagtcgcta tttaatccgt catctctttc tcggttttgc
     6301 cgccgctgca gggctattgc tgccgctttt taccaccttc aacctgatta acgaactgga
     6361 tggtgtcagc ccgggcggtt atcgctggac tcaggcggtg ctggtggtgc taatgacctt
     6421 accgccattc gcagcacggg gttttctatc ttccgtattg cactggtggc gctggttgcc
     6481 gggatattgt ggactgtttc gttaggcgcg atagatgagt gggtggcgtc gccattgcag
     6541 caacaggcgt tgcaaatcaa atcgaccgcc accgcgttgg gggaggacga tgacattacc
     6601 ggcaatatgc tgtgggccag gcgtggtaat gaatttgtga cggtgaaatc gctgaacgag
     6661 cagggccagc cggtgggcgt ggagatattt cattatcgcg acgatctctc gcttgaatcc
     6721 tacatttatg cacgcagtgc caccattgaa gacgacaaaa cgtgggtcct gcatggtgtg
     6781 aatcataaaa aatggctgaa tggcaaagaa acgctggaaa cattagataa tcttgcctgg
     6841 caatcggcct tcaccagtat gaatcttgaa gagttatcaa tgccggggaa tactttttct
     6901 gtccgtcagc ttaatcatta catccattat ttgcaggaaa ccgggcaacc cagcagtgaa
     6961 taccgccttg cactgtggga aaaactgggg caacctatcc tgaccctggc gatgattttg
     7021 ctggctgtgc cgtttacctt tagtgccccg cgttcgcctg ggatgggtag ccgtctcgct
     7081 gtaggcgtca tcgtgggctt actcacctgg atcagctatc aaatcatggt taatctggga
     7141 ttgctatttg cgttaagcgc acctgttacc gcgctcggtt taccggtagc gtttgtgttg
     7201 gtggcgttga gcctggtgta ttggtatgac agacaacatt aaacccgccg tggcgcaaac
     7261 accacggcgg gctcacatta ctgcttcaga gtagcaaatg cctgaatgat gacatcgatt
     7321 tgctcacgac tgtgggcagc atttacgcta cagcgcagca gcgtgatgcc tgcaggcgcg
     7381 gcaggcggca ggataagatt cacatacacg cccagcgaga taagctcgcg ccagatgcgt
     7441 aaaccttcct ctttcgaacc gattattact ggcactaccg ggctaatatg tgaacttaac
     7501 tcataaccca gtttttgcag accgtcatac agatggttgg cgttatccat cagcttttgc
     7561 cgcagctctg gttgtgtggc aatagttctt aacgacgagc gcacggtcgc aatgcaggac
     7621 ggagaaggag aggcggtaaa aatatacggg cggctgccgt agcgcagcac ttccatcgct
     7681 tccgaaccga cagcaaaacc gccaatcgag gccagacttt tactgaaagt accaacgata
     7741 atatccacat catcttcaac gccgacagct tcagccagtc cgcgccccgt tgcgcctaac
     7801 acgccaaacg aatgcgcttc atcgacaatc aaataaccac cgagacgacg tttgatatca
     7861 acaatttccg ccagcggcgc aacgtcaccg agcatgctgt agatgccctc aacgatgata
     7921 atcgcctctt tcgcccgttc accgaggcga accatgcgcc gttccagatc tttagcatcg
     7981 ttatggcgga aacgaataat ttccgcgcca ccgagagaac aggcgtcata aatactggcg
     8041 tggctgtcgg catccagtaa caccacggcg ttatggtcgg caagtgcgct gatgacgcct
     8101 aaattagcgg tataaccggt tgaaaaaacg atagcggtag ggcgattgaa aaactcggca
     8161 atctctttct ctaacgccag gtgcggcgca taactgccgt ttgccatacg agaaccggtg
     8221 gtgccggtgc cttgggccgc taacgcggcc tgaccttcgc tgatggcatc atgattaaag
     8281 gttaaaccga gataattatt ggtcccggcc agaatcactt ttttattgcc gatccggcct
     8341 tcggtggcgg aatacacttc atcaatacag gtgccaaacg gtgttaaccc gttgtcacta
     8401 aattgcagac gctcgccagc aaggcgcgca tatttatcgt atagccccat tatttattct
     8461 ccagccatgg aagtagagtt tccattaatt gcgcaggggt tttgacatcc agcagaatat
     8521 taataggaat ggaaatatcg aatctgtctt ccagcatcat taagagatcc atcactttga
     8581 tcgactccag gccaagatcg ttgacgagat cgctgtcggg cttaatttcc acaccgtttt
     8641 cgaccaaatc ctgtaaacag gaaaggatgt aatccattac tatttcacga tttaccatag
     8701 aaacgttaac acacctttaa acaagacaat caaaatagcc cgttgcgtaa tgcgtgttcc
     8761 aggctaatcc cgggaaacca attaatatct tcagaaatac ggttattact tgccgaccag
     8821 tcggcgtggg ttaattcacg aattttcgag cgggtcagca taggttcttt acctgccagg
     8881 cgactcaacg cggtactgat atccgcaagg cctgtgagta ccggcagagg aatgtcaacc
     8941 attcgcacgg aaccacaacg ggctttggcg gcaagctgtc gtacgcgttg ccagtcatag
     9001 ccaccggcga cgccatcgca taattcatag gtttgcgtct gtacagtttc ggcgcttaac
     9061 cactgaccca ctgcttgcgc aaaatcggtg acgtgaagaa aagagagttg tgtatctggc
     9121 gcaccaagtc gtggcagcag gccgcgtagc atccagtcaa acagcggttt taactcttta
     9181 tcgccgggac catacacggc tgtcgggcga aaaacgccca gcgtaatttc gtccgccatt
     9241 gctgccagtc gttgctcggc gacgtatttg gattttgcat accaggagag ctcgggatgg
     9301 cgcgccgcca gcgaagagat aaacagaaaa cgttggcaaa agccgctctc ttttgctgct
     9361 tgcatcaggc gcaggctgcc gtcaacgtta cagcgggtga aaatctcttc tttgtgcccg
     9421 cgcacttgcc cggcgcaatg gaccactacg ctggctccgg caaccagctc gctaagtgaa
     9481 tgagtatctt ccagcgaacc gcgcacccag gtgagattat cattgacgtg agcgcgggca
     9541 gtacgcgtca atgcgcgaac gtgaaagccg cgggcgagca ggttatcgat aatatattta
     9601 ccgataaacc cggtagcgcc cgtcaccgcg acagtttgat tcatgccagg gattcctgca
     9661 cattaagact ggcagcataa gccttctgat aacgtttttt cgcttccgca cgggcaggct
     9721 tgccggagga cgttcgggga atactgtggg gcggcaacag atcgatagcc gcggtcacgc
     9781 caaattcgct ttggatccgt gccgccagcg cgtggataag ctgcccgcga cgttcttcgt
     9841 cgctgatccg acactggatc tgcaaaatga ttttttcctg ggcggtaaca aaagcaatcg
     9901 catcgccaga atgaatttcc ggttcctgtt ccgctatata ttcaatatcc tgcggccaga
     9961 tattacggcc acgaataata atcagatctt taatgcgtcc ggtgacatac agataaccgt
    10021 ccagcagata accgaggtcg ccggtgtcta accagcccgt cgcggcaatc tcgtcttgcg
    10081 aaacctggtc gccaaagtaa ccgctcatca gactgggacc ggagatgcaa atatggccta
    10141 ccacacgttc cgcgaccggc atacctgctt cattgcggat ttcaatacca tgttccggca
    10201 acgctttgcc gcagttgacg aaagtcgata cggcgcgtgt ctctgcaccc ggcgcgacgg
    10261 ctttaccctg atattcgagg atgtcgcgat ccacttcgtt aaccacaacc ccggaggctt
    10321 catcagagaa gctgacagcc agcgcatttt ctgccagtcc gtagcacggc atgaaagttt
    10381 tattgtcaaa gttaacctga cggaaacatt cagcgaattg atggagttgt tctgcggaga
    10441 tcggctcagc accaatacca gcgacgcgcc agcaggaaag atccagttca gcgagatctt
    10501 tttcattcac gcggcgttgg cacaattcat agccaaacgg cggcgcaacg gaaacggtgc
    10561 cgcgattttt actgatcaat ttaagccatt gcaaaggacg catggcaaaa tcctgagtgc
    10621 gcaaataatc tactgaaagt tgcgtggcga cggggtcagg agaaagccga ccagtcccat
    10681 atcatggtag aaaggctgcc aggagacgca gcggtcgcta gggcgtaatt taatgccatc
    10741 gtggcttata gcacgtagat tagccatcac ttcgcgatgg gggataatga cgccacgggg
    10801 aaaacgggtg ctgccggagg tgtactggag gtaggcgata tcgttcggaa ctggacgctg
    10861 gagcgcaaca tcggcttccg gtaatgcctt aaaccaagcg tggcttaaaa catgtaattc
    10921 ggggttgtta tgcgtcgcgg cattgaccag tggcaaccac tcatcaccag tgataatggc
    10981 tgcgggctgg caacttgcta gtaaaccctg gagtttggcg ctccaggaat cccgctgacc
    11041 gacgcccatt ggaatcgcca acgggacggc gactaagcca gcatactgac aggcaaaaaa
    11101 agcctctaca aacccgctac tcgtttcggc aatcagcgcc acgcgttcgc cttttttcag
    11161 gttcagcgat aacaaccgct tcgcaccagc ttcggcacgc gtttttaatg tctgatattc
    11221 cagttgatct tcaagttggc aacgtctgtc ataaaaattc attccggcgc tactcagagc
    11281 ggcgtagtcc aaagcatcaa ccagcgttgg aaaatcggca tagcgcatag gtagggaatg
    11341 cgtaaagatt ttattagaca tatacaccac gaaaaaattg gatatcaaaa ataaaatgac
    11401 aataatgcgg tggattatat aggcataaaa gataaatgag atagtatttt catcggtgtg
    11461 aaaatgaaca ttgatttaaa taatgagttt aagttttttg tttttattat gaatgatatt
    11521 taatttatga atatattaag aagaagcctt gtccggtcgc cttaaatcga caaaaggcat
    11581 tgaggcgttt ctgtgtagcc tctgacctta tcataatcat taattaatac tatttattcg
    11641 tcgtttgtat tcattgagtt gattattaag atgccgctcg ttgctgttgc aacctgtttt
    11701 tcaccattaa ttatttgtta tccacagaga tatggaatgc gatctgctga tgattaaaat
    11761 tgaaaaagta attaataaaa atgaccttaa ggcatttatc gcttttccgt catcacttta
    11821 tcccgacgat ccaaactgga tacccccttt attcattgaa cgcaacgagc atttgtctgc
    11881 gaaaaatccg gggacggacc atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca
    11941 gatagttggg cgtattaccg cgcaaataga taccttgcac cgtgagcgtt acggtgaaga
    12001 taccggtcat ttcggcatga ttgacgccat tgatgatccg caggattttg ccgcgttgtt
    12061 tggcgcagcg gaagcgtggt tgaagtcaca aggtgcaagt aagatcagcg ggcctttcag
    12121 cctgaatatc aatcaggaaa gcggattact gattgaaggt tttgacacac caccctgtgc
    12181 gatgatgcca cacggcaaac cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa
    12241 aggcattgat ttactggcgt ggtggatgca gcgaaccgat ctcactttct ctccggcgct
    12301 aaaaaaactg atggatcagg tgcgcaaaaa ggtgaccatt cgctgcatca atcgtcagcg
    12361 gtttgccgaa gagatgcaga tcctgcgtga gattttcaac tctggctggc agcacaactg
    12421 gggatttgtg ccgtttaccg aacatgaatt cgcgaccatg ggcgaccaac ttaaatatct
    12481 ggtgccggac gatatgatct acatcgctga gattgattct gcaccctgcg cgtttattgt
    12541 cggcttgcct aacatcaacg aggcgattgc cgatctgaac ggatcgctct ttcccttcgg
    12601 ctgggcaaaa ttgctgtggc gtttgaaagt cagcggtgtg cgaactgcgc gagtaccgct
    12661 gatgggcgtg cgcgacgagt atcagttcag ccgcatcggc ccggtgattg cgctgttatt
    12721 gattgaagcc ttacgcgatc cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg
    12781 gatcctcgaa accaatacag gcatgaataa catgctggaa cgcattgggg cggagccgta
    12841 caagcgttac cgattgtatg aaaagcaaat ttaataccag atcctgttta gcgcaggggc
    12901 ggtcattcgg gagagggtat agatgaacag atttaacacc agatcctgtt aagtgcaggg
    12961 gcggtcccct cgccttgggg atagggtaaa actctgctca cattcccacc ttgttcatct
    13021 ccagcggcac cgccgagcga atcaaatggt ggccttccag ctcgatatcg tggagattct
    13081 ttttcacggt ccgtacatga tcgcgcgctg cgcgctgagc gacgtgcggc agccgctgca
    13141 acaccgcgtt gtagatccgc gcatgctggc gatcgatctg ctttttttgc tgtggtcgat
    13201 ggtagagatt atttacgctg gcaaacactg aattaaacat cagatcggtc aatgattgca
    13261 gcgtaaacac cagcacctga ttgtgagaag cctgacaaat ggcgagatgg aaagcgtgat
    13321 ccaactgcgc atgttcgatc agcgaaatct ctttgttgtt ctcactggcg gcgagcattt
    13381 tttcataaca gcgggttatc acaacaaaat cagcctgcgt tcccagcgtt gccgccagcc
    13441 ttgccgattc gccctccagt aatgcgcgaa cgtcgagcag atcgtacagc gttcgcggct
    13501 gcgtactgaa cagatggatc agcgggctgg tgtcctgcac ccgattaagc cgtgcgacac
    13561 gagaatcgcg accctgcgcc gtttcaataa tcccgcgccc gcgcagcacg gtcagccctt
    13621 cacgcagtgc ggagcgtgag aagccgagct tttcacacag tcgacgttcc gagggaagcg
    13681 gctgaccgac cttcagtacg ccgtcgataa ttaaccgttc gatactctct gcaaccactt
    13741 cgcaaatagg gcgacgttca tctttcattc ccggacctcg tgcacaggta ggaccaattt
    13801 ttctgttctt aactcgcaaa acacgcacat cacgtaagtg attatgttac atcaatttaa
    13861 cattaagtta accaagacaa ggtcacagag ctggaaaaaa aatggtctga ccggtagcta
    13921 aagagataga cgaaaacgaa aagcccgctt aataactgtt cacagaagca gcgcgcaaaa
    13981 atcagctgcc acacaacaca acaaagcgaa gcctactcat gagcatcttg tacgaagagc
    14041 gtcttgatgg cgctttaccc gatgtcgacc gcacatcggt actgatggca ctgcgtgagc
    14101 atgtccctgg acttgagatc ctgcataccg atgaggagat cattccttac gagtgtgacg
    14161 ggttgagcgc gtatcgcacg cgtccattac tggttgttct gcctaagcaa atggaacagg
    14221 tgacagcgat tctggctgtc tgccatcgcc tgcgtgtacc ggtggtgacc cgtggtgcag
    14281 gcaccgggct ttctggtggc gcgctgccgc tggaaaaagg tgtgttgttg gtgatggcgc
    14341 gctttaaaga gatcctcgac attaaccccg ttggtcgccg cgcgcgcgtg cagccgggcg
    14401 tacgtaacct ggcgatctcc caggccgttg cgccgcataa tctctattac gcgccggatc
    14461 cttcctcaca aatcgcctgt tcaattggcg gcaatgtggc ggaaaatgcc ggtggcgtcc
    14521 actgcctgaa atatggtctg accgtacata acctgctgaa aattgaagtg caaacgctgg
    14581 acggcgaggc gctgacgctg ggatcggacg cgctggattc acctggtttt gacctgctgg
    14641 cgctgttcac cggatcggaa ggtatgctcg gcgtgaccac cgaagtgacg gtaaaactgc
    14701 tgccgaagcc gcccgtggcg cgggttctgt tagccagctt tgactcggta gaaaaagccg
    14761 gacttgcggt tggtgacatc atcgccaatg gcattatccc cggcgggctg gagatgatgg
    14821 ataacctgtc gatccgcgcg gcggaagatt ttattcatgc cggttatccc gtcgacgccg
    14881 aagcgatttt gttatgcgaa ctggacggcg tggagtctga cgtacaggaa gactgcgagc
    14941 gggttaacga catcttgttg aaagcgggcg cgactgacgt ccgtttggca caggacgaag
    15001 cagagcgcgt acgtttctgg gccggtcgca aaaatgcgtt cccggcggta ggacgtatct
    15061 ccccggatta ctactgcatg gatggcacca tcccgcgtcg cgccctgcct ggcgtactgg
    15121 aaggcattgc ccgtttatcg cagcaatatg atttacgtgt tgccaacgtc tttcatgccg
    15181 gagatggcaa catgcacccg ttaatccttt tcgatgccaa cgaacctggt gaatttgccc
    15241 gcgcggaaga gctgggcggg aagatcctcg aactctgcgt tgacgttggc ggcagcatca
    15301 gtggggaaca tggcatcggg cgagaaaaaa tcaatcaaat gtgcgcccag ttcaacagcg
    15361 atgaaatcac gaccttccat gcggtcaagg cggcgtttga ccccgatggt ttgctgaacc
    15421 ctgggaaaaa cattcccacg ctacaccgct gtgctgaatt tggtgccatg catgtgcatc
    15481 acggtcattt accttttcct gaactggagc gcttctgatg ctacgcgagt gtgattacag
    15541 tcaggcgctg ctggagcagg tgaatcaggc aattagcgat aaaatgccgc tggtgattca
    15601 gggcagcaat agcaaagcct ttttaggtcg cccagtcacc gggcaaacgc tggatgtgcg
    15661 ttgtcatcgc ggcattgtta attacgaccc gaccgagctg gtgataaccg ctcgtgccgg
    15721 aacgccgctg gtggcgattg aagcggcgct ggagagcgcc gggcaaatgc ttccctgcga
    15781 gccgccgcat tatggtgaag aagccacctg gggcgggatg gtcgcctgcg ggctggcggg
    15841 gccgcgtcgc ccgtggagcg gttcggtccg cgattttgtc ctcggcacgc gcatcattac
    15901 cggcactgga aaacatctgc gttttggtgg cgaagtgatg aaaaacgttg ccggatacga
    15961 tctctcacgg ttaatggccg gaagctacgg ttgtcttggc gtgctcactg aaatctcaat
    16021 gaaagtgtta ccgcgaccgc gcgcctctct gagcctgcgt cgggaaatca gcctgcaaga
    16081 agccatgagt gaaatcgccg agtggcaact ccagccatta cccattagtg gcttatgtta
    16141 cttcgacaat gcgttgtgga tccgccttga gggcggcgaa ggatcggtaa aagcagcgcg
    16201 tgaactgctg ggtggcgaag aggttgcggg tcagttctgg cagcaattgc gtgaacaaca
    16261 actgccgttc ttctcgttac taggtacctt atggcgcatt tcattaccca gcgatgcgcc
    16321 gatgatggat ttacccggcg agcaactgat cgactggggc ggggcgttac gctggctgaa
    16381 atcgacagcc gatgacaatc aaatccatcg catcgcccgc aacgctggcg gtcatgcgac
    16441 ccgctttagt gccggagatg gtggctttgc cccgctatcg gctcctttat tccgctatca
    16501 ccagcagctt aaacagcagc tcgacccttg cggcgtgttt aaccccggtc gcatgtacgc
    16561 ggaactttga ggagcaggct atgcaaaccc gattaaccga agagatgcgg cagaacgcgc
    16621 gcgcgctgga agccgacagc atcctgcgcg cctgtgttca ctgcggattt tgtaccgcaa
    16681 cctgcccaac ctatcagctt ctgggcgatg aactggacgg gccgcgcggg cgcatctatc
    16741 tgattaaaca ggtgctggaa ggcaacgaag tcacgtttaa aacacaggag catctcgatc
    16801 gctgcctcac ttgccgcaat tgtgaaacca cctgtccttc tggtgtgcgc tatcacaatt
    16861 tgctggatat cgggcgtgat attgtcgagc agaaagtgaa acgcccactg ccggagcgaa
    16921 tgctgcgcga aggattgcgc caggtagtgc cgcgtccggc ggtcttccgt gcgctgacgc
    16981 aggtagggct ggtgctgcga ccgtttttac cggaacaggt cagagcaaaa ctgcctgctg
    17041 aaacggtgaa agctaaaccg cgtccgccgc tgcgccataa gcgtcgggtt ttaatgctgg
    17101 aaggctgcgc ccagcctacg ctttcgccca acaccaacgc ggcaactgcg cgagtgctgg
    17161 atcgtctggg gatcagcgtc atgtcagcta acgaagcagg ctgttgtggc gcggtggact
    17221 atcatcttaa tgcgcaggag aaagggttgg cacgggcgcg caataatatt gatgcctggt
    17281 ggcccgcgat tgaagcaggt gccgaggcaa ttttgcaaac cgccagcggt tgcggcgcgt
    17341 tcgtcaaaga gtatgggcag atgctgaaaa acgatgcgtt atatgccgat aaagcgcgtc
    17401 aggtcagtga actggcggtc gatttagtcg aacttctgcg cgaggaaccg ctggaaaaac
    17461 tggcaattcg cggcgataaa aaactggcct tccactgccc gtgtacccta caacatgcgc
    17521 aaaagctgaa cggcgaagtg gaaaaagtgt tacttcgtct tggatttacc ttaacggacg
    17581 ttcccgacag ccatctgtgc tgcggttcag cgggaacata tgcgttaacg catcccgatc
    17641 tggcacgcca gctgcgggat aacaaaatga atgcgctgga aagcggcaaa ccggaaatga
    17701 tcgtcaccgc caacattggt tgccagacgc atctggcgag cgccggtcgt acctctgtgc
    17761 gtcactggat tgaaattgta gaacaagccc ttgaaaagga ataaaaaaat gaaaactaaa
    17821 gtcattctta gccagcaaat ggcgagtgca attattgccg caggtcagga agaggcgcag
    17881 aaaaataact ggtctgtttc cattgctgtt gccgatgacg gcggtcatct gctggcgtta
    17941 agtcgcatgg acgattgcgc gccgattgcg gcttacatct cccaggagaa agcgcgtacc
    18001 gccgcgctgg ggcgtcgtga aactaagggc tatgaagaga tggtgaacaa cggacgtacc
    18061 gcgttcgtga ctgcgccgtt actgacgtcg ctggaaggcg gcgtaccggt tgttgttgat
    18121 gggcaaatta ttggtgccgt gggcgtttct ggtttaaccg gagcacagga tgcgcaggtc
    18181 gcgaaagcgg cagcagcggt gttggcgaaa taagcgaaaa cgaggagaga aacaatgagt
    18241 caaaccataa cccagggccg tttacgcatt gacgccaatt ttaaacgttt tgtggatgga
    18301 gaagttttgc cgggcgtgga actggacgct gctgcattct ggcacaatgt tgatgagatc
    18361 gttcacgatc tggcaccaga aaatcgtcag ttgctggcag aacgcgatcg cattcaggcg
    18421 gcgcttgatg agtggcatcg cagcaatccg gggccggtaa aagataaagc ggcctataaa
    18481 tctttccttc gtgaactggg ctacctggtg ccgcaaccgg agcgcgtgac ggtggaaacc
    18541 acgggcattg acagcgaaat caccagtcag gcggggccac agctggtggt tccggcaatg
    18601 aacgcccgct acgcgctgaa cgcggcgaac gctcgctggg gctcactgta cgatgcgtta
    18661 tacggcagcg acatcatccc gcaggaaggg gcgatggtca gtggctacga tccgcagcgc
    18721 ggtgagcagg ttatcgcctg ggttcgacgt ttcctcgatg aatcactacc gctggaaaac
    18781 ggcagctatc aggatgtggt ggcgtttaag gtggtcgata aacaattacg catccagttg
    18841 aaaaatggta aagaaaccac gttacgtact ccagcacagt ttgtcggtta ccgtggcgat
    18901 gccgctgcgc cgacctgcat tttgctgaaa aataacggcc tgcatattga actgcaaatt
    18961 gatgccaacg ggcggattgg caaagacgat ccggcgcaca tcaacgatgt tatcgtcgaa
    19021 gctgctatca gtaccattct cgactgcgaa gattcggtcg cggcggttga tgcggaagat
    19081 aaaatcctgc tgtaccgcaa tctgctgggc ctgatgcagg ggactctgca agagaaaatg
    19141 gagaaaaacg gtcggcaaat cgtacgtaaa ctgaatgacg atcgtcagta caccgccgcc
    19201 gatggctctg aaatttctct gcacggacgc tcgttgctgt ttatccgcaa cgtgggtcat
    19261 ttgatgacca ttcctgtgat ttgggacagc gaaggcaatg aaatcccgga aggtattctt
    19321 gatggcgtca tgactggcgc gattgccctc tatgatttaa aagtgcagaa aaactcgcgc
    19381 actggcagcg tctatattgt gaaaccgaaa atgcacggtc cgcaggaagt ggcgttcgcc
    19441 aacaaactgt ttacccgcat tgagacaatg ctcggtatgg caccgaatac cctgaaaatg
    19501 ggcattatgg atgaagaacg ccggacctcg ctgaacttgc gtagctgtat cgctcaggcg
    19561 cgcaaccgcg tggcgttcat caataccggt ttcctcgacc gtaccggcga tgaaatgcat
    19621 tcggtgatgg aagctggccc gatgctgcgt aaaaaccaga tgaaatcgac gccgtggatc
    19681 aaagcctacg aacgtaataa cgtgctttcc ggtctgttct gtgggctgcg cggtaaagcg
    19741 caaatcggta aaggcatgtg ggcaatgccg gacctgatgg cagacatgta cagccagaag
    19801 ggcgaccaac tgcgtgccgg ggcaaacaca gcctgggttc cgtcaccaac cgctgctacg
    19861 ctccatgcgc tgcactacca ccaaaccaac gtacagagcg tgcaagccaa cattgcccag
    19921 accgagttca atgctgaatt tgaaccgctg ctggacgatc tgctgactat tccggtggct
    19981 gaaaacgcta actggtcggc gcaagagatc caacaagagc tggataacaa cgtgcagggg
    20041 attctggggt acgtggtgcg ttgggtggag caggggattg gttgttcaaa agtgccggat
    20101 attcacaacg tggcgctgat ggaagaccgc gcaacgctgc gtatctccag ccagcatatc
    20161 gccaactggt tacgtcacgg tattctgacc aaagaacagg tgcaggcgtc gctggagaat
    20221 atggcgaaag tggttgatca gcaaaacgct ggcgatccgg cttatcgtcc gatggcgggg
    20281 aatttcgcta actcgtgtgc ttttaaagct gccagcgatt taatcttcct cggcgtgaaa
    20341 cagccaaacg gttataccga accgttatta cacgcctggc gtttacgcga aaaagaaagt
    20401 cattaatagc gtcaaaaaag ccccggcaag ttttattgtc ggggctatga tagtaactgc
    20461 ggtagtacat ttatcccgga aataaccgga gcgattatca gggtttaaca atattaaata
    20521 cccgtagc
//
LOCUS       DADLSB010000063        20521 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.063, whole genome shotgun sequence.
ACCESSION   DADLSB010000063 DADLSB010000000
VERSION     DADLSB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.063"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JN331_002212"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JN331_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY7260348.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JN331_002213"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JN331_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY7260349.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JN331_002214"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JN331_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY7260350.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN331_002215"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN331_002215"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY7260351.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN331_002216"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN331_002216"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY7260352.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGREPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="JN331_002217"
     CDS             4449..4814
                     /locus_tag="JN331_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY7260353.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4964..5482
                     /locus_tag="JN331_002218"
     CDS             4964..5482
                     /locus_tag="JN331_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY7260354.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="JN331_002219"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="JN331_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="JN331_002220"
     CDS             6713..7195
                     /locus_tag="JN331_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY7260355.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JN331_002221"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JN331_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY7260356.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JN331_002222"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JN331_002222"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY7260357.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JN331_002223"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JN331_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY7260358.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JN331_002224"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JN331_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY7260359.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JN331_002225"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JN331_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY7260360.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10864..11730
                     /locus_tag="JN331_002226"
     CDS             10864..11730
                     /locus_tag="JN331_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY7260361.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="JN331_002227"
     CDS             11751..13037
                     /locus_tag="JN331_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7260362.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JN331_002228"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JN331_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY7260363.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="JN331_002229"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="JN331_002229"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY7260364.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="JN331_002230"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="JN331_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY7260365.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="JN331_002231"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="JN331_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY7260366.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="JN331_002232"
     CDS             complement(16985..17275)
                     /locus_tag="JN331_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY7260367.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JN331_002233"
     CDS             complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JN331_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY7260368.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="JN331_002234"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="JN331_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY7260369.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="JN331_002235"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="JN331_002235"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="JN331_002236"
     CDS             19137..20378
                     /locus_tag="JN331_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7260370.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKKRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5299 a         5357 c         4819 g         5046 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcattaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgctaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacaggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggctcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaagcaatt tggtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt tgaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcaaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aggagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cacccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgata tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca ttcccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccaa ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgcta
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcagtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacccggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gagtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgccccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagaaacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DADLSB010000064        20441 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.064, whole genome shotgun sequence.
ACCESSION   DADLSB010000064 DADLSB010000000
VERSION     DADLSB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.064"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..214)
                     /locus_tag="JN331_002237"
     CDS             complement(<1..214)
                     /locus_tag="JN331_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HAY7260371.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKE"
     gene            complement(222..932)
                     /locus_tag="JN331_002238"
     CDS             complement(222..932)
                     /locus_tag="JN331_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY7260372.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1148..2971)
                     /gene="typA"
                     /locus_tag="JN331_002239"
     CDS             complement(1148..2971)
                     /gene="typA"
                     /locus_tag="JN331_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY7260373.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            3344..4753
                     /gene="glnA"
                     /locus_tag="JN331_002240"
     CDS             3344..4753
                     /gene="glnA"
                     /locus_tag="JN331_002240"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY7260374.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEAAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            4926..5975
                     /gene="glnL"
                     /locus_tag="JN331_002241"
     CDS             4926..5975
                     /gene="glnL"
                     /locus_tag="JN331_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY7260375.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            5987..7396
                     /gene="glnG"
                     /locus_tag="JN331_002242"
     CDS             5987..7396
                     /gene="glnG"
                     /locus_tag="JN331_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY7260376.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            7508..7618
                     /gene="yshB"
                     /locus_tag="JN331_002243"
     CDS             7508..7618
                     /gene="yshB"
                     /locus_tag="JN331_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY7260377.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(7847..9220)
                     /gene="hemN"
                     /locus_tag="JN331_002244"
     CDS             complement(7847..9220)
                     /gene="hemN"
                     /locus_tag="JN331_002244"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY7260378.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(9409..9918)
                     /gene="yihI"
                     /locus_tag="JN331_002245"
     CDS             complement(9409..9918)
                     /gene="yihI"
                     /locus_tag="JN331_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY7260379.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            10500..11132
                     /locus_tag="JN331_002246"
     CDS             10500..11132
                     /locus_tag="JN331_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY7260380.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(11514..14300)
                     /gene="polA"
                     /locus_tag="JN331_002247"
     CDS             complement(11514..14300)
                     /gene="polA"
                     /locus_tag="JN331_002247"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY7260381.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            14302..14544
                     /locus_tag="JN331_002248"
     CDS             14302..14544
                     /locus_tag="JN331_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260382.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHAVKNKSIPNN"
     gene            14664..15596
                     /locus_tag="JN331_002249"
     CDS             14664..15596
                     /locus_tag="JN331_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7260383.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(15637..17067)
                     /locus_tag="JN331_002250"
     CDS             complement(15637..17067)
                     /locus_tag="JN331_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7260384.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKNRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(17222..17848)
                     /gene="dsbA"
                     /locus_tag="JN331_002251"
     CDS             complement(17222..17848)
                     /gene="dsbA"
                     /locus_tag="JN331_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY7260385.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(17865..18851)
                     /gene="srkA"
                     /locus_tag="JN331_002252"
     CDS             complement(17865..18851)
                     /gene="srkA"
                     /locus_tag="JN331_002252"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY7260386.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHTGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(18928..19197)
                     /locus_tag="JN331_002253"
     CDS             complement(18928..19197)
                     /locus_tag="JN331_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY7260387.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            19267..19851
                     /gene="mobA"
                     /locus_tag="JN331_002254"
     CDS             19267..19851
                     /gene="mobA"
                     /locus_tag="JN331_002254"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY7260388.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            19833..20360
                     /gene="mobB"
                     /locus_tag="JN331_002255"
     CDS             19833..20360
                     /gene="mobB"
                     /locus_tag="JN331_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY7260389.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         4969 a         5165 c         4951 g         5356 t
ORIGIN      
        1 gttctttatc gtccatatta ccaagagaaa aacgctcgcc atccagaaca tggattttga
       61 cgccacgtag attattttca cttgcgatat caagtaaatc agcaggcaac actctttcca
      121 gccgcatatt taaatgaaag gcataagcat gtaaataaag aggaagagtg tcgacacgtt
      181 gtagaatctt tcttgcgtta tttattgtca ccataaaaca cctacagatt aatgatgagg
      241 tctacggcct caaatcgcca atattcaata tctatctgga caatattgcc ttgctggtcg
      301 gcgcgatgct tttcaattaa gatcgctggc atacccgcag aaccgccaag atacttgctg
      361 atatcgcccg gcattctgac gggtttgaaa atcaattttt ttactaccgt ctctttttgg
      421 tagcactttt cccagactgc agaaaatgag tggttttcaa gttgttcaat aaaacccgga
      481 gcaacttcag gattaatata tgtttcgtga tagaaaactt tatgtccttc cagcgccccc
      541 cagccggtga tgcgatagag ttcagttgat ggcgtcactg caatcagtgg cgcgagcgtc
      601 tcgggaatat cgctggtacg gtttttctcg gtaaaccccc aagaaggctc tcgcccttgc
      661 tcaattgcgg cccgctgaaa gctcgccgac agctccggac tgtaattaaa tcgcggctgg
      721 gtcacgaacc accccttacg atccttacgg aagattttgg attctgcctc gaggttcaac
      781 aacgcctggc gcagcgtcat acgtttaatg cccagcagtt ctcccagctc tctttctgaa
      841 gggagttttc cacccggcgt ggtgatgcca tctttcaacc accgatccag tttctctttt
      901 gcattttcta cggtggattg attctccgcc attgtttaat ccttttgatt taaaccagat
      961 gaagcatctt acgcttacgc caaaaaagaa agcaattaac ggtgtatgac tttgcagata
     1021 attgtgacaa agctctctat tgacgtaatc acagacttat aagggagata ctcgcattgg
     1081 tgtgcgctca cgacaggtaa aaaaaacctg ccatcgctgg caggtttttt atgactaaaa
     1141 aacgaaatta atcgtctttc ggtgcgcggt tggcgcggcg acgatcgttt tccgtcaggt
     1201 gacgtttacg aatacggata gaggtcggag tcacttctac cagttcgtca tcatcgatga
     1261 actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaaca gcttcgtcag
     1321 taccggaagc acgcatgttg gtcagtttct taccggtcag gcagtttaca gtcaggtcgt
     1381 tagagcggct atgaataccg ataatctgac cttcgtaaac ttctgcaccg tgaccgagga
     1441 acagcttacc gcgatcctgc agaccgaaca gcgcgaacgc gacagcttta ccctgaccgt
     1501 tagagatcag tacaccgttc tgacgctgac ccacttcgcc cggacgtacg tcgtcgtagt
     1561 ggctgaaggt ggagtacagc agaccagtac cggaagtcat ggtcatgaac tcagaacgga
     1621 agccaatcag accacggctt gggatcacgt agtcgagacg tacgcggcct ttaccgtctg
     1681 gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcatcaca gaaccctgat
     1741 gctgttcttc aacgtccagc gtcacgtttt catacggctc ttgtttacga ccgtcgattt
     1801 cacggaagat tactttcgga cgggataccg ccagttcgaa accttcacga cgcatgtttt
     1861 cgataagaac agagaggtgc agttcaccac gaccagaaac gcggaacgca tcggcgtctt
     1921 cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga cgatcgagga
     1981 tctgacgaga cgttacgaac ttaccttctt taccgcagaa cggcgaggtg ttaacgcaga
     2041 agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca acgttttgcg
     2101 tgtcgcaaac ggtgtcagaa atgttcagtt cgcccaggcc cgtgatcgcc acgatatcgc
     2161 cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc actttaccga
     2221 ctttcgcgtt acgggttttg ccttcgctat cgatgatagt aacctgctgg ttcggcttca
     2281 ctttaccacg cttgatgcgg ccaatgccga taacgccaac atagctgttg taatcgagct
     2341 gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga acgtggtcaa
     2401 caatcgcctg atacagcggg gtcatgtctt ccgccatatc ttcgtggtcc agacccgcga
     2461 taccgttcag cgcagaagcg taaacgatcg ggaagtccag ctgctcgtcg gtcgcgtcga
     2521 ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg ccagggcggt
     2581 caactttgtt gataacaaca atcggcttca ggccgtaagc aaacgctttt ttggttacga
     2641 agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact gagtctacca
     2701 tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta tcaacgatgt
     2761 tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta atcccacgct
     2821 ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga gagtcgaacg
     2881 taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct acgtgcgcga
     2941 tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta gaaatagcgc
     3001 gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct ccgcaaacaa
     3061 gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta tccaaaggtc
     3121 attgcaccaa tatggtgctt aatgtttcca ttgaagcact atattggtgc aacattcaca
     3181 tcatggtgca gcccttttgc acgatgttaa gcatgataac gccttttagg ggcaatttaa
     3241 aagttggcac agatttcgct ttatcttttt tacggcgaca cggccaaaat aattgcagat
     3301 ttcgttacca cgacgacaat gaccaatcca ggagagttaa agtatgtccg ctgaacacgt
     3361 actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca ccgataccaa
     3421 aggtaaagaa caacacgtca ctatccctgc tcatcaggtg aatgctgaat tcttcgaaga
     3481 aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg aatccgacat
     3541 ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg actccaccct
     3601 gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc gtgacccgcg
     3661 ctctattgcg aagcgcgccg aagattacct gcgttccact ggcattgccg acaccgtact
     3721 gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgtttcggat catctatctc
     3781 cggttcccac gttgctatcg acgatatcga agccgcatgg aactcctcca cccaatacga
     3841 aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg ttccgccggt
     3901 agactctgct caggatattc gttctgaaat gtgtctggtg atggaacaga tgggtctggt
     3961 ggttgaagcc catcaccacg aagtagcgac tgctggtcag aacgaagtgg ctacccgctt
     4021 caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgtcg tgcataacgt
     4081 agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa ccgatgttcg gtgataacgg
     4141 ctccggtatg cactgccaca tgtctctgtc taaaaacggc gttaacctgt tcgcaggcga
     4201 caaatacgca ggtctgtctg agcaggcgct gtactacatt ggcggcgtaa tcaaacacgc
     4261 taaagcgatt aacgccctgg caaacccgac caccaactct tataagcgtc tggtcccggg
     4321 ctacgaagca ccggtaatgc tggcttactc tgcgcgtaac cgttctgcgt ctatccgtat
     4381 tccggtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg atccggcagc
     4441 taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggta tcaagaacaa
     4501 gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccag aagaagcgaa
     4561 agagatccca caggttgcag gctctctgga agaagcactg aacgaactgg atctggaccg
     4621 cgagttcctg aaagccggtg gcgtgttcac ggatgaagcg atcgatgcgt acatcgctct
     4681 gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt ttgagctgta
     4741 ctacagcgtc taatagttga agttgtacta cccggcgcac aacgccggga tttagttgcc
     4801 gtggaaactt tcagcccatc tctgcatggg cttttttctc cgtcaattct ctgatgcttc
     4861 gcgcttttta tccgtaaaaa gctataatgc actaaaatgg tgcaacctgt tcaggagact
     4921 gctttatggc aacaggcacg cagcccgatg ctgggcagat cctcaactcg ctgattaaca
     4981 gtattttgtt aattgatgac aatctggcga tccattacgc caaccccgcc gcgcaacaac
     5041 tgctcgccca aagctcccgc aaattgtttg gtacgccgtt accggaactg ttgagctact
     5101 tctcattaaa tatcgagctg atgcaagaaa gtctggaggc ggggcaaggc tttaccgata
     5161 acgaagtgac gctggtcatc gatggtcgct cgcatatcct ttctgtgacg gcccagcgta
     5221 tgccggacgg catgatcctg ctggagatgg caccgatgga taaccagcgc cgcttaagcc
     5281 aggaacaact acaacatgct caacaagtag ctgcccgtga tttagtgcgc ggcctggcac
     5341 atgagattaa aaatccgctt ggcggtttac gtggcgcggc gcagttactc agcaaagcgt
     5401 tacctgaccc atcactactc gaatatacca aagtgattat cgaacaggcg gaccggctgc
     5461 gaaatttggt cgaccgtctg ttggggccgc agctgcccgg tacgcgcgtt accgaaagta
     5521 ttcacaaagt ggctgaacgc gtggtaacgc tggtgtcgat ggaactgccg gacaacgtgc
     5581 ggttgattcg tgattacgat cccagcctgc cggaactggc gcacgacccg gatcaaattg
     5641 aacaggtctt gctgaatatt gtgcgcaatg cgctacaggc gctggggccg gaaggcggtg
     5701 aaatcattct gcgtacccgc accgcgtttc aactgacctt acacggcgag cgctaccggc
     5761 tggcggcgcg gattgatgtg gaagataacg ggccgggcat tccgcctcat ttgcaggata
     5821 cgctgtttta cccgatggtc agcggccgcg aaggtggcac cgggcttggc ttatccatcg
     5881 cccgtaattt gattgatcag cattcaggca aaattgaatt taccagttgg ccaggtcata
     5941 ccgagttctc ggtttacctg cctatcagga aataaaggtg acgtttatgc aacgagggat
     6001 agtctgggta gtcgatgacg atagttccat ccgttgggtg cttgaacgtg cgctcgctgg
     6061 agcgggttta acctgtacga catttgagaa cggcgcggaa gtactggagg cgctggcgag
     6121 caaaacgccg gatgtgctgc tttcagatat ccgtatgccg ggaatggacg ggctggcgct
     6181 gctcaagcag attaaacagc gccatccaat gcttccggtc atcattatga ccgcacattc
     6241 cgatctggat gctgccgtca gcgcctatca acaaggggcg tttgattatc tgcccaaacc
     6301 gtttgatatc gacgaagcag tggcgctggt tgagcgcgct atcagtcatt accaggaaca
     6361 gcagcagccg cgtaatattc agcttaacgg cccaacgacc gatatcatcg gcgaagcgca
     6421 agccatgcag gacgtgttcc gtattatcgg tcggctttcg cgttcttcta ttagcgtgct
     6481 gattaacggc gaatccggca ccggtaaaga actggtcgct catgccctgc atcgccacag
     6541 tccgcgcacc aaagcgccgt ttatcgcgct gaatatggca gctatcccaa aagatttgat
     6601 cgaatcagaa ctgtttggcc acgagaaagg cgcgtttact ggcgcgaata ccattcgtca
     6661 ggggcgtttt gaacaggccg atggcggcac gttatttctc gacgaaattg gtgatatgcc
     6721 gctggatgtg cagacgcgtt tgctgcgcgt gctggcagac ggtcagtttt atcgcgttgg
     6781 cggctatgcg ccggtgaaag tggatgtgcg gattatcgct gccactcacc agaatcttga
     6841 acagcgggtg caggaaggta agttccgtga ggatctgttc caccgcctga acgttatccg
     6901 cgttcatctg ccgccgctgc gcgaacgtcg ggaagatatt ccccgtctgg cgcgccattt
     6961 tttacaggtt gccgcgcgcg aactgggcgt agaagcgaag ttgctgcatc cggaaaccga
     7021 agctgctctg acgcgtctgg cgtggccagg caacgtgcgc cagctggaaa acacctgccg
     7081 ctggctaacg gtgatggccg ccgggcagga agtgttgatt caggatttgc ccggcgaact
     7141 gtttgaatca acggttgcgg agagtacttc gcaaatgcaa ccggacagtt gggcgacact
     7201 tttagcgcag tgggcagaca gagcgctgcg ttccggtcat caaaatctgc tttccgaagc
     7261 gcagccagag ctggagcgga cgttactgac gaccgcgttg cgacatacgc aggggcataa
     7321 acaggaagcg gcgcggctac tcggctgggg ccgcaacact ctgacgcgta agttaaaaga
     7381 gctggggatg gagtgattca cggcttgtgt gtaaagattg attattgagc gcaaattgct
     7441 ggtattttgc gctttactgt tccgataagt tcagtatgat cttgcccgga aaacggggag
     7501 agtcattatg ctggaatcaa taattaatct ggtatcgagt ggcgcagttg acagccacac
     7561 accgcaaact gccgttgctg ccgtgctgtg tgccgcgatg attgggctgt ttagctgaag
     7621 ctcgttgttt atgccggatg cggagaaagt cgtaggcctg ataagacgcg accagcgtcg
     7681 catcaggcat catgctcaga tgcctgatgc ggcgtaaacg ccttatccgg cctacaaaag
     7741 caataatttc aacaggtttt gttgcccagc gcatcaggca atcttgttct cagcacattg
     7801 atggcgcttt cgttttctac tttgtaaacg aagcgccatt cactctttaa atcacccgtg
     7861 agaactgctg catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc
     7921 ggatcagcaa gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc
     7981 catcttttgc taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca
     8041 aatcccactg tttctcaaga ggggcgtaat ccagacggaa gttgcagatg agcgacttaa
     8101 tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc
     8161 cttgttcatc cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt
     8221 cgccaatcat gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg
     8281 tgtagccctg gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat
     8341 caggacgggc aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga
     8401 tggtttcctg caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt
     8461 tgcgctgagc agcaaaaatg gtcggcagat gcgcgtagtt aaaaacgctc agacgatcgg
     8521 ggttcagctc cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca
     8581 ggccgtaaat caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca
     8641 gtgcaaagat gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga
     8701 agtcctgcac gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat
     8761 ccagttcgat ttcccgcgga tcgacttcga tcgaaatctc cgcatcggta ttgaaatgga
     8821 agttttcgcg cagcagcttc atcagacggc tgatttgcgc tttattcaga tacgtcggcg
     8881 ttccgccgcc ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga
     8941 cgatttcttg ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa
     9001 tcttattgca accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag
     9061 ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt
     9121 ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct
     9181 ggatcagggc cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg
     9241 tctctggcgg ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag
     9301 ccacttttgt cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat
     9361 aaccggaagg gttatcagca gaacgggaag gcccacggcg gcaatccgtt agttgccccg
     9421 cagcagacgc atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata
     9481 agagaggccg agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga
     9541 ttgctcttcg gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg
     9601 ctcatccgtt tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt
     9661 cggtttgtgc tgtttggtga ctttttcagt cactcccaat ggaatagggg ttttactgcc
     9721 aatacgtgga tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc
     9781 tgcgcggctg cccggcgcat gaccacgacg ttttttctga cgcttacggt cacgagcttc
     9841 ctgatccagc tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt
     9901 tgaagatgat ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg
     9961 gcggaatcta acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt
    10021 tcctgactca taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt
    10081 tttccattag ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg
    10141 tttgtcttcc tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt
    10201 cattgttcct gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa
    10261 acaatcaacc gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg
    10321 atttatatag cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact
    10381 atcggcaatg tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg
    10441 ccttttatta tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt
    10501 tgactaattt gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc
    10561 taccttccga taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca
    10621 gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc
    10681 gcacccagct tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg
    10741 ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg
    10801 aatacctcga aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc
    10861 cgctgaaaga tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc
    10921 tggtgctgct gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata
    10981 tggtgcgtga agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt
    11041 tgaagaaaca aggcgtagac aagctgcggc agaaactgga tacctggttt agcgagatgc
    11101 agcctgtaga agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat
    11161 gtggcgtatc cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat
    11221 agcgcatcag gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact
    11281 gactggggcg gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga
    11341 tagaacatct tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga
    11401 aagcactttt tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa
    11461 acgtgacagc ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc
    11521 gcctgatccc agttttcgcc actccccact tccaccagca acggcacatc cagacgggta
    11581 cagttttcca tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact
    11641 tcaaatacca gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt
    11701 aaccacgcat caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt
    11761 ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata
    11821 tccggcagat acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga
    11881 gcacgggtgc gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg
    11941 tacttctgcg cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg
    12001 ccataaatca gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt
    12061 tccagtggca aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg
    12121 aatgcggtca gcaagccttt atcacgcgag agatgcgcca taatgcgcag ttcaatctgc
    12181 gagtagtccg ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga
    12241 cgaccttctt cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt
    12301 cccgttgcag ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc
    12361 atcagcggca gcttgtcggt gtaagtcgac ttcagcttcg ccagaccacg atactccaga
    12421 atcacttttg gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac
    12481 ggcgcgccac ccggcgtttt cttcagcggt ttaataccct gtttttcaaa cagaatggtt
    12541 tgcaactgct tggtggaaga aaggttaaat tcttcacctg caatttcatg cgctttcttt
    12601 tccagctcag ccagacgcaa ggtgagctct tcagaatgat tgtgcagcac tttcggatcg
    12661 atcttcacac cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc
    12721 tcgaagacgt tcaacggccc tttgtgtttt tgcagatccg gccacatttt cagatgcaac
    12781 tgcaaggtga catctgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg
    12841 ttaaaggtca gttgattttt gcctttaccc gcaatctctt caaaagtgat ggttttgtgc
    12901 ttcaaccaac gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg
    12961 taggactcca gcatggtatc aaacgcaatc ccacgcagtt caatgccgta attcgccaga
    13021 ataccgcgat cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc
    13081 ggttttagca actcgagtgc acgctcgtga gagatttgat cgggcgcatc aagataatca
    13141 tgagcaaccg gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg
    13201 ttagcagaga tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt
    13261 tccagcttcg caatccacgc tttcagtgtt tcttcatcaa ggatagtgac gtagttgtca
    13321 taagaaatca ccgttgccgt cacttctggc gcttcatctg caacactggt ttcctgcggc
    13381 ttcgcggctg gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc
    13441 cagcgtttga actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc
    13501 acttccagtt gttcacaggt cagctccagt tcaacatcgg ttttaatcgt cgccaactgg
    13561 tatgagagat aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca
    13621 cggaagctca acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga
    13681 ccttgcagca atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatca
    13741 gaggagtcac ccatcagcgc caggaagtcg atgatcaatt ctggcggcac gccgtactta
    13801 ttcaccacct cttccggtcc gaggatggta ttcgtcatgg tattgataag cgtaatattt
    13861 ggcgtcacca gctgcgccat atctttatcg ccagtgctaa tcagcaccgg acgcccggct
    13921 ttttcggctt cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc
    13981 agcagcggca gtcccatcgc tttaaccatc gcgtgcagag gttcgatttg tgctcgcaga
    14041 tcgtccggca ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt
    14101 tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg
    14161 cgcagcatgt tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc
    14221 gggggaaacg cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga
    14281 ttttggggga tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc
    14341 cacagacagg atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct
    14401 gctggatcgc tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt
    14461 ttaatttatt atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa
    14521 aataaatcca tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgaa
    14581 ttatttttct ccttaaggat catccgttat ttgggtcatt ttctgctaaa atctgcgcct
    14641 tgcgcttctt tagtgcactg tctatggcta accttttgaa taaattcatt atgacgagaa
    14701 tactcgctgc gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt
    14761 gttccgtccc gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt
    14821 ggcgaaaggt ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg
    14881 tattactgca tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta
    14941 agaagaactg gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt
    15001 gcgtgctgtt tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct
    15061 gggtgccatt tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt
    15121 cccgcgctta tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc
    15181 gttcttgtga aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc
    15241 gtttcacgca agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa
    15301 aggcggcagg cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta
    15361 atgttacgct gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta
    15421 aactgacgcg gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg
    15481 actacatcaa tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc
    15541 aggaaaaaga ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt
    15601 tactcgcctc tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg
    15661 agacataaat tcttcttcgc tcatctcctg tccgttaaaa acaactttac ccggtgtata
    15721 acgcagctgc agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacctaa
    15781 tgtcatcatc ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc
    15841 ttcagataaa ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc
    15901 aaaattgagc gatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat
    15961 actgatatcc agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt
    16021 aatggtcggc tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac
    16081 ttcttgcaga gcaacttcat cgtttgctaa ctcagggtgt gcagcaagct gcttctgcat
    16141 cgcaatgtta tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat
    16201 caatgaccac tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta
    16261 ggtcagattg acattgatat aatctttgtc tttaacgcga tccagcctgt tttttgcaac
    16321 gaaagcatcg atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt
    16381 cgactcgctt tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt
    16441 aaaacttttt gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt
    16501 gccttcaata tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc
    16561 accaaaagaa aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc
    16621 accaaacgcg aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag
    16681 cggttgtgtc agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac
    16741 attaccatgc attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac
    16801 aactttctgg cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat
    16861 ttgaaaacgg gaattaaaaa agcctttgtc aaagttttta taacttaacg tcatatcgta
    16921 ggcatgttca cctttctttt tcgcatcgtt aaaatcttta ataaactttt cgataccagg
    16981 ctgaatttgc gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat
    17041 aacacctgta gctgacttac gtatcatata aatattccct gataaaacag caaaactttg
    17101 aatatccact tatgctgaaa taatgtccag cggcaggatg cattatcaga atgtatttat
    17161 atttattaaa taataaaaaa agcccgtgaa tattcacggg ctttatgtaa tttacattga
    17221 attatttttt ctcggacaga tatttcaccg tatcagcata ctgctgaaca aaaacatcca
    17281 tattgctggt atccataccc tgcggattca gctgatattt accgttaaca aacatcgccg
    17341 gaacaccacg caattgcaca tcagctgcgg ctttttcctg ctgagcgacc agagatttca
    17401 ccacgaagct gttccacgcc gcgtcgtact cttcaccttt aatacctgca ttgataaata
    17461 catcgcggat atcagatgct gaacgaatgg tctgggtttt ctgtacgcct tcaaacagcg
    17521 gaacagtcac tttgtcttcc acgcccagcg ccatcgccac agcccatgcc tgagtcagat
    17581 ctttgcccag gtcaccaccc atgaagttga cgtggtattt agtcatcttc acgccttccg
    17641 gcagtttttt cttcacatta tcagaaatat gcagaacttc ttcaaactga tagcagtgcg
    17701 ggcagaagaa agagaaaaac tccagcactt gcggcgcgcc agctaccggt ttttccaggg
    17761 tagtgtactg tttaccatct tcatactgcg ccgccgatgc gctaaacgct aaaactaaac
    17821 cagccagcgc cagccaaatc tttttcatga tctactctct ccgattaata cataggtgtt
    17881 aattgcaaag ggggttcttg tagaactttt gcctgttcta taaaagtcgc cgtctgtcgt
    17941 agccagtaat cttccccggt taaccacggg aaatttttcg ggaacgcggg atcagcccaa
    18001 tgccgcatta gccaggcaag ataataaacc aaacgcatgg cgcgtaaagg ttcaatcagt
    18061 ccgatttcag cggtgtcgaa ctcgctaaat tcttcataag cttcaataat agtttccagt
    18121 tgcatccgct gctcggcttt atcgccattg agcaacatcc acaaatcctg aatagctgga
    18181 ccattacgtg catcatccag atcaacaaac attggaccat cgcgccagag aatattcccg
    18241 gtgtggcagt ctccatgtag ccgcagaacg gtgaaatctt cccgccagtg tgctgtaacg
    18301 gcggcgatca gctcatctgt cgctttcaga aatgccgctt tcaacccgga aggtatcagt
    18361 gtagcgtcct caaacagctt gcgaggctca atgagatatt cattcagacc gatggtcggg
    18421 cgatgaataa aaagctgttt gcgccccgtc tggtgcatac gccctaaata acgcccaacc
    18481 gcttccatct gatcgatatt atcagcttcg aactggcgac caccgacgct tggaaaaaca
    18541 gcgaaataaa atccctgatg attcaataaa gtctgaccgt taaaggccac aggtgctgcg
    18601 accggaactt catcatttac cagctgcaac gcaaattgat gttcttcgag gatttgatcg
    18661 gctgtccaac gttcagggcg ataaaatttg acgacaaaac gtcgacgctc ttcgtcctga
    18721 aattgataga cacggttttc atagctgtta agcggggtaa gaccggaatc cacccggatc
    18781 ccttgctcaa acagagcgtc catgatggta tccgggtgta gtgtctggaa agtaaaagcg
    18841 ctgttgttca tcccatcatc cggaaaacat tacgaatgat gaaggatatc atttcacgat
    18901 gattttggtg gcgccgctta caagctttta ctctttaatt acgccgcgag cgcgtaacag
    18961 cgccgttttg aagtcttctt cataatcttt ctgcaaaccc gggatcaccg catctttcgc
    19021 ggaatcacgc attttcaggt gatagatcag aatgtcatcc gtcaaatccg ccagttcgcc
    19081 gtcaaaacct gactctttcg ccagtttctg caaaaattgc aacaagttaa agtctggctc
    19141 tttttgccag gctggctgga ggagttcaat aacttcattc agacgtttac atttcatagt
    19201 aatgctcctt actcttgaga cagacacgtt agcagggtca atcccacaat aaaagaggcg
    19261 atatcggtga atctgatgac gacgataaca ggcgttgtgc tggcaggcgg taaagccaga
    19321 cgaatgggcg gcgtagataa aggattgctg gacttaaatg gcaaaccgct gtggcaacat
    19381 gtcgctgacg cgcttaagac gcagctctct cacgtcgtgg ttaatgctaa tcgtcatcag
    19441 gaaatctatc aggcaggcgg tctgaaagtg attgaagatt cactggcgga ttacccaggc
    19501 cctctggcag gaatgctttc agtaatgcag caggaagctg gtgagtggtt tttgttttgt
    19561 ccgtgcgata cgccttacat tccccatgat ttagccgccc ggcttaatca tcagcgcaaa
    19621 gatgcgcctg tcgtgtgggt ccatgacggt gaacgcgatc acccgactat tgctctggta
    19681 aaccgcgcta ttgagccttt attactggaa tatctgcaag caggagaacg ccgggtaatg
    19741 gcatttatgc gtctggctgg cggtcatgcg gttgatttca gcgatcataa agatgcattt
    19801 gttaacgtga atacgccaga ggagcttgcc cgatggcagg aaaaacgatg ataccgttac
    19861 tcgcctttgc cgcgtggagt ggtaccggaa aaactacgct tttgaaaaaa ctgatcccgg
    19921 cattatgcgc cagagggatc cgtccggggc tgattaagca tacgcaccat gatatggatg
    19981 ttgataagcc aggcaaagat agctatgagc tgcgcaaggc tggcgcggca caaaccatcg
    20041 ttgccagcca gcaacgatgg gccttgatga cggaaacacc agacgaagaa gagctggatc
    20101 tacattttct cgcaagtcga atggatacct caaagctgga tttgattctg gtcgaagggt
    20161 ttaagcatga agagatcgca aagattgtgc tgtttcgcga tggagccgga catcgacctg
    20221 aagaattagt gatagacagg catgttattg ctgtagccag tgatgtgccg cttaatcttg
    20281 atgtcgcatt actggatatt aatgatgttg aggggctggc tgattttgtg gtggagtgga
    20341 tgcaaaagca gaacggataa gcaagagtag gctggatcag acgcagccag tgtcgcatcc
    20401 gacctttttc tgcaccagcg caaaaaggcc cgactgggcc t
//
LOCUS       DADLSB010000065        20403 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.065, whole genome shotgun sequence.
ACCESSION   DADLSB010000065 DADLSB010000000
VERSION     DADLSB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.065"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(234..929)
                     /gene="aqpZ"
                     /locus_tag="JN331_002256"
     CDS             complement(234..929)
                     /gene="aqpZ"
                     /locus_tag="JN331_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY7260390.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLINIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1354..3012
                     /locus_tag="JN331_002257"
     CDS             1354..3012
                     /locus_tag="JN331_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY7260391.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGFSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(3009..3965)
                     /locus_tag="JN331_002258"
     CDS             complement(3009..3965)
                     /locus_tag="JN331_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY7260392.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4116..5231
                     /gene="macA"
                     /locus_tag="JN331_002259"
     CDS             4116..5231
                     /gene="macA"
                     /locus_tag="JN331_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY7260393.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5228..7175
                     /gene="macB"
                     /locus_tag="JN331_002260"
                     /pseudo
     CDS             5228..7175
                     /gene="macB"
                     /locus_tag="JN331_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
     gene            complement(7248..7472)
                     /gene="cspD"
                     /locus_tag="JN331_002261"
     CDS             complement(7248..7472)
                     /gene="cspD"
                     /locus_tag="JN331_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY7260394.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7795..8115
                     /gene="clpS"
                     /locus_tag="JN331_002262"
     CDS             7795..8115
                     /gene="clpS"
                     /locus_tag="JN331_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY7260395.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8146..10422
                     /gene="clpA"
                     /locus_tag="JN331_002263"
     CDS             8146..10422
                     /gene="clpA"
                     /locus_tag="JN331_002263"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY7260396.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTAALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10825..10912)
                     /locus_tag="JN331_002264"
     tRNA            complement(10825..10912)
                     /locus_tag="JN331_002264"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10876..10878),aa:Ser,seq:gga)
     gene            complement(11166..11384)
                     /gene="infA"
                     /locus_tag="JN331_002265"
     CDS             complement(11166..11384)
                     /gene="infA"
                     /locus_tag="JN331_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY7260397.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11669..12373)
                     /gene="aat"
                     /locus_tag="JN331_002266"
     CDS             complement(11669..12373)
                     /gene="aat"
                     /locus_tag="JN331_002266"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY7260398.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12415..14136)
                     /gene="cydC"
                     /locus_tag="JN331_002267"
     CDS             complement(12415..14136)
                     /gene="cydC"
                     /locus_tag="JN331_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY7260399.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14137..15903)
                     /gene="cydD"
                     /locus_tag="JN331_002268"
     CDS             complement(14137..15903)
                     /gene="cydD"
                     /locus_tag="JN331_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY7260400.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(16026..16991)
                     /gene="trxB"
                     /locus_tag="JN331_002269"
     CDS             complement(16026..16991)
                     /gene="trxB"
                     /locus_tag="JN331_002269"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY7260401.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17536..18030
                     /gene="lrp"
                     /locus_tag="JN331_002270"
     CDS             17536..18030
                     /gene="lrp"
                     /locus_tag="JN331_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY7260402.1"
                     /translation="MVDSKKRSGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18165..>20403
                     /gene="ftsK"
                     /locus_tag="JN331_002271"
     CDS             18165..>20403
                     /gene="ftsK"
                     /locus_tag="JN331_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7260403.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIALFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPV"
BASE COUNT         5158 a         5075 c         5425 g         4745 t
ORIGIN      
        1 gcacccccgg gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt
       61 cggcaaccgg gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat
      121 gtgggctgcc ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc
      181 ggtttgtaat gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca
      241 cgcttttcca gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc
      301 acccagaaga accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg
      361 ctgcgcgccg ggttaacaga agtgttagtc accggaatat tgattaagtg aatcagggtt
      421 aaggccagac caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg
      481 tggatcacta acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg
      541 gaataaccgc ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg
      601 tcaaaacccg ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg
      661 acaacctggg caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat
      721 aaaccaatag tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc
      781 atcgtcagaa cggtcaaacc aaatgccagc gccacgccgg caaaacctat gcctaattcc
      841 gggaagcctg cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca
      901 aaacattcag ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa
      961 aaatatggtc gttttatagg gtcgtcgtaa aaaatgacga cggaaataat gcgcggctat
     1021 tttaaaaacg aaggcgagtc attcaccaga taaataaatc cagtaaattt gatttagggc
     1081 aacagcgggt tgccccatat agtcatttgt ctgattgaca gtgtagtgca agcaaaagat
     1141 ttaatccttt aggcgtaata aaaaataatt tatcatgcta attatttgat tttgttgttt
     1201 ttgcgggctt atcagcaaga gggagtataa cgcgattatt cgctcatttt tcagacaatt
     1261 gccatgctta aatgtgatgt catcacgtat tagcaaggcc tttcccgtta tactgccagc
     1321 gtaaaggata agtcacatat ttctggaggg gatatgattc ttgagcgcgt tgaaattgtg
     1381 ggttttcgcg gtatcaaccg tttgtcgttg atgctcgaac aaaacaacgt cctgattggg
     1441 gagaacgcgt ggggtaaatc cagcttgctg gacgccttaa ccctgctgct atcgccagaa
     1501 tcagatctct accattttga gcgcgacgat ttctggttcc cgccgggaga tatcaacggg
     1561 cgagaacatc atctgcatat tattttgacc ttccgcgaat cgctgccagg ccgacatcgg
     1621 gttcgccgtt atcggccgct ggaagcgtgc tggacgccat gcaccgatgg ctatcaccgt
     1681 attttttatc gtctggaagg ggagagtgcg gaagacggca gcgtgatgac actgcgcagt
     1741 tttctcgata aagacggaca tccgattgat gttgaggata ttaacgatca ggcacgccat
     1801 ctggtgcgtt taatgccggt gctgcgcttg cgtgatgccc gttttatgcg ccgtattcgt
     1861 aacggcacgg tgccaaatgt ccccaatgtg gaagtcaccg cgcgccagct cgatttcctc
     1921 gcccgtgagt tgtcctcaca tccgcaaaat ctctctgatg gacagattcg tcagggactt
     1981 tccgcaatgg tgcagttgct tgagcattat ttctctgagc agggggctgg acaggcgcga
     2041 tatcgtttaa tgcggcggcg ggccagcaat gagcaacgaa gctggcgcta tctggacatc
     2101 atcaaccgga tgattgaccg acctggaggg cgctcgtatc gggttatttt gctcggcctg
     2161 tttgctactt tattgcaggc aaaaggcaca ttgcgactgg ataaagacgc gcgtccgtta
     2221 ttgctaattg aagatccaga aacccgcctg catccgatta tgctttccgt tgcctggcat
     2281 ctgttgaatc ttttgccttt acagcgtatt gctaccacca actctggcga gctgctttcg
     2341 ttaactccag tggaacatgt ttgccgcctg gtgcgtgaat cttctcgtgt tgccgcctgg
     2401 cgtctggggc caagtggctt tagcaccgaa gacagccgcc gcatctcttt tcacattcgt
     2461 tttaatcgtc cgtcatcgct gtttgctcgc tgctggttgc tggtggaagg ggaaacggaa
     2521 acctgggtta tcaatgaact ggcgcgtcag tgcggacatc attttgatgc cgaagggatc
     2581 aaggtcattg agtttgccca gtccgggcta aagccactgg ttaaatttgc ccgccgaatg
     2641 gggattgaat ggcatgtact ggtcgatggc gatgaagcag ggaagaaata tgccgctacg
     2701 gtacgtagcc tgttgaataa cgatcgggaa gccgaacgag aacatttaac ggcgttaccg
     2761 gcgctggata tggaacattt tatgtatcgc cagggatttt ccgatgtgtt ccaccgcgtg
     2821 gcgcaaatcc cggaaaatgt accgatgaat ctacgcaaaa ttatctcgaa agcgatccat
     2881 cgctcttcca aacccgatct tgccattgaa gtggcaatgg aggcaggacg tcgtggtgtg
     2941 gactccgtac caacgctgct gaaaaaaatg ttctcacgcg tgctgtggct ggcgcgcggt
     3001 cgcgcggatt aaccgcgaaa catcgtggcc atttgtggct gaatagcgtc gagcatctca
     3061 tagcgccgac ggtattcagc ccgtttttta ctggcgattt cggcaatctc ttttcgtgct
     3121 atctgtgctg gaaggcggta atggcgttca gcatcacata cgccgccaac cgactcccag
     3181 aacgcgttgt aatcagcatg gatcttgcct tctttatcgc gataacgcag gctgcggtaa
     3241 atatgcgttt cattgctgac ggcaataatc tgctctacct gcaaacgttg ggcaaacaga
     3301 caggccgctt ccatcacgag gcgtttggga aatagcccgt ggcaggcttt cgtcgcattc
     3361 tggatttcct gatgtggaat ttcccatttt gcgccttgca gtccgccaat aaacatcgtt
     3421 cttttcccct gatattcaca cagggtaaac gtgatctctg ccagaggaat accttcgctg
     3481 ttgcggaaca ggattgtgct gtcaccttct ttatccattg agatcatcat ggtcagctca
     3541 agcgtgaact gctcaccgtt tttgccttcc agtttagcca gttgcagccc gggggtattc
     3601 aaatataagc tgaattcttc cgccgacata cacccgcgga gtaacgcata atggtaacgt
     3661 aacgcctcca gcaattgctt acggctaaga ttcgccgcaa ggtaagggcg atgcagacgc
     3721 acaggcagtc gcggctggcg cgttaacaac acattgagat taggccagtg ggaaagttcg
     3781 ttcatccact caacgcttaa acgcggcata atcaacgagc gcagcaaaaa tttctggcga
     3841 aaactacagc gatgccagaa tttacccggc cgacactgtc cacgtgccag actaagaaaa
     3901 agtgacaggc tgctgagaga ttcagatggc gtaaaggtcc gttcagttag ctgcgacata
     3961 ttcatgaaat caatggttat acatgacgtc gatttcacca ttgcgtatct taaccaaaca
     4021 tcaatagtgt gattactaac gtaaatttta gggttttgtt gatatttcgt tgaagttaat
     4081 gacccggatt ggcatatgga gtattcagaa tatttatgaa aaagcggaaa accgtgaaga
     4141 agcgttacgt tattgcgctg gtgatagtca tcgccggact gattacgtta tggagaattc
     4201 ttaacgcacc cgtgccgact tatcagacac tgattgtgcg ccccggtgat ttacagcaaa
     4261 gcgtgctggc gaccggaaag ctggacgcgc tgcgtaaggt tgacgtgggc gcgcaggtca
     4321 gcggtcagtt gaaaacgctg tcggtggcga ttggcgataa agtaaaaaaa gaccagcttt
     4381 taggggtcat tgatcctgaa caggctgaaa accagatcaa ggaggtcgaa gcaacgctga
     4441 tggagctacg cgcgcagcgg cagcaggcgg aagcggagct gaaactggcg cgggtgacgt
     4501 attcccgtca gcaacgtctg gcacaaacgc aagcggtttc actgcaggat ctcgataccg
     4561 ccgcgacgga gatggctgtg aaacaggcgc aaattggcac cattgacgcg caaatcaagc
     4621 gcaatcaggc ctctctcgat acggctaaaa ccaatctcga ttacacccgt atcgttgccc
     4681 cgatggccgg ggaagtgacg caaatcacca ctctgcaagg ccagacggtg attgccgcac
     4741 agcaagcacc gaacattctg acgctggcag atatgagcac tatgctggta aaagcgcagg
     4801 tttctgaagc ggatgtaatc cacctgaagc cggggcaaaa agcctggttt acggtgcttg
     4861 gcgatccact gacgcgctac gaggggcaaa tcaaggatgt gctaccgacg ccggaaaagg
     4921 taaacgacgc tattttctat tacgcccgtt ttgaagtacc caaccccaat ggtttgctgc
     4981 ggctggatat gaccgcgcaa gtgcatattc agctcaccga tgtgaaaaat gtgttgacga
     5041 tccctctgtc ggcgttaggc gatccggttg gcgataatcg ttataaagtc aaattgttgc
     5101 gtaatggtga aacacgcgag cgtgaagtga cgattggcgc acgtaacgat accgatgttg
     5161 agattgtcaa agggctggaa gcgggcgatg aagtggtgat tggtgaggcc aaaccaggag
     5221 ctgcacaatg acgcctttgc tcgaattaaa ggatattcgt cgcagctatc ctgccggtga
     5281 tgagcaggtt gaggtgctga agggcatcag cctcaatatt tatgcgggtg agatggtcgc
     5341 gattgttggc gcttcgggtt ccggtaaatc gaccctgatg aatattctcg gctgcctgga
     5401 taaggccacc agcggcacct atcgcgtagc cggtcaggat attgccacgc tggacgccga
     5461 tgcgctggcg caactgcgcc gcgagcattt cggctttatt ttccagcgtt accatttgct
     5521 ttcgcattta accgccgagc agaacgttga agtacccgcc gtctatgccg gtcttgagcg
     5581 gaaacagcga ctgcttcgtg cccaggagtt gctgcaacgg ctggggctgg aagaccgtac
     5641 agagtattat ccggcacagc tttcgggtgg tcagcaacag cgcgtcagca tcgcgcgggc
     5701 attgatgaac ggcggtcagg taattcttgc cgatgaaccg accggcgcac tggacagcca
     5761 ttctggcgaa gaggtgatgg cgatcctgca tcagctgcgc gatcccgggc atacggtgat
     5821 tatcgtcacc cacgatccgc aggtcgctgc tcaggccgag cgggtgatcg aaattcgcga
     5881 cggcgaaatt gtgcgcaatc ctcccgccat tgaaaaagtg aatgttgctg gcgggacgga
     5941 gcctgttgtc aatacagtgt ctggctggcg gcagtttgtc agcggtttta acgaggcgct
     6001 gacgatggca tggcggacgc tggcagcgaa taaaatgcgt actttactga ccatgctggg
     6061 gattattatc ggtattgcgt cggtggtttc cattgtcgtg gtgggtgacg ccgccaaaca
     6121 aatggtgctg gcggatattc gttctattgg tacgaatact attgatgtct atcccgggaa
     6181 agattttggc gatgacgatc cgcaatatca gcaggcgctg aagtacgacg acttaatcgc
     6241 catccaaaaa caaccgtggg tcgcctcagc cacacctgcc gtctcgcaaa acctgcgcct
     6301 gcgttataac aatgttgatg ttgctgccag tgccaatggc gtgagcggcg attattttaa
     6361 tgtctatggc atgacacttc agtgaaggaa acacctttaa tcaggagcag ctgaacggtc
     6421 gtgcgcaggt cgtggttctc gacagaaata ctcgccgcca gcttttcccc cataaagcag
     6481 atgtggttgg cgaggtgatt ctggtcggca atatgcccgc cagagtcatt ggtgtggcgg
     6541 aagaaaaaca gtcgatgttt ggtagcagta aagtgctgcg tgtctggcta ccttacagca
     6601 cgatgtccgg gcgagttatg ggccagtcgt ggcttaactc cattactgtc agggtgaaag
     6661 aaggatttga cagcgccgag gcggaacagc aactcacgcg tttactttca ctgcgccacg
     6721 gtaagaagga tttctttacc tggaacatgg acggcgtctt gaaaactgtt gaaaagacca
     6781 cacgtacttt acaactgttt ctgacgctgg tggcggtgat ttcgctggtg gtgggcggta
     6841 ttggtgtaat gaatattatg ctggtgtcag tgaccgagcg gacgcgggaa attggcattc
     6901 gcatggctgt aggtgcgcga gcaagcgatg ttttgcaaca gttcctgatc gaagccgtac
     6961 tggtttgcct ggtcggtggc gcgttgggaa taacactgtc actgttaatt gctttcacct
     7021 tgcagctttt cttacccggc tgggagattg gtttttcacc gttggcgctg ctgctggcgt
     7081 ttctctgctc gacggccacc gggattttat ttggctggtt acccgcacga aatgcggcac
     7141 gactggatcc agtagatgct ctggcacgag agtaattttt gagataaaaa tgccagccga
     7201 tcgggctggc attttgcctt taggatgtac acaatgagac agaagagcta tgcgactgcc
     7261 gcttctactt cgacgggcac aataacactg gcgtgattgc cttttggccc ctggtggaca
     7321 tcaaactgaa cggattgtcc agcttttagc gttctgtaac catccatctg aatggtggaa
     7381 taatgagcga aaatatcttc gccgccgcct tcagggcaga tgaaaccaaa ccctttggca
     7441 ttgttgaacc acttaacagt acccttttcc atgcttcgac atccttcgca aatcttatac
     7501 aagtaagatg gaataaaccg gggtcagaga gggggctgtt caaaacctcg ccaactctag
     7561 aaatacaatt tagagaatta gggcgagccg tcaagcattt gacaggggcc agggggcagg
     7621 tatgaatcaa aaatttgaag cagttaacgc tattgacagg aatgtgacag atgtcgctga
     7681 tgccaacgat agatgatagt tatctatcat gtggagtaga ttggtcaggc aaataagctc
     7741 ttgtcagcgg cagggcgttc tgccgataac cgtaaccgaa gatgataact gacaatgggt
     7801 aaaacgaacg actggctgga ctttgatcaa ctggcggaag aaaaagttcg cgacgcgcta
     7861 aaaccgccat ctatgtataa agtgatatta gtcaatgatg attacactcc gatggagttt
     7921 gttattgacg tgttacaaaa attcttttct tatgatgtag aacgtgcaac gcaattgatg
     7981 ctcgctgttc actaccaggg gaaggccatt tgcggagtct ttaccgccga ggttgcagaa
     8041 accaaagtgg cgatggtgaa caagtacgcg agggagaatg agcatccatt gctgtgtacg
     8101 ctagaaaaag cctgaatgca ggcataaaaa ttgggggagg tgcctatgct caatcaagaa
     8161 ctggaactca gtttaaatat ggctttcgcc agagcgcgcg agcaccgtca tgagtttatg
     8221 accgtcgagc acttgttact ggcgctgctc agtaacccat ctgcccggga ggcgctggaa
     8281 gcgtgttctg tggatttggt tgcgctccgt caggaactgg aagcctttat tgaacaaacc
     8341 acacccgttc tgcctgccag tgaagaggag cgcgacacac agccgacgct gagttttcag
     8401 cgtgtactgc aacgtgcggt cttccatgtc cagtcctccg gtcgcaatga ggttaccggt
     8461 gcaaacgttc tggtcgctat cttcagcgaa caggagtcgc aggcggcata tctgttgcgt
     8521 aaacatgaag tcagccgtct cgatgtggtg aactttatct ctcatggcac gcgtaaagac
     8581 gagccgacac agtcttctga tcctggcagc cagccaaaca gcgaagaaca agctggtggg
     8641 gaggaacgta tggagaattt cacgacgaac ctgaaccagc ttgcgcgtgt gggcggaatc
     8701 gacccactaa ttggtcgtga gaaagagctg gagcgtgcta ttcaggttct ctgccgtcgc
     8761 cgtaaaaaca acccgctgct ggtgggggaa tctggtgtcg gtaaaaccgc gattgcagaa
     8821 ggtcttgcct ggcgaattgt tcagggtgat gtgccggaag tgatggctga ctgtacgatt
     8881 tactctctcg atatcggttc tctgttagcg ggcacaaaat atcgcggcga ctttgaaaaa
     8941 cgttttaaag cgttgctcaa gcagctggag caggacacta acagcatcct gtttattgat
     9001 gagatccaca ccattatcgg tgcgggtgca gcgtctggtg gtcaggtcga tgcggctaac
     9061 ctaatcaaac cgttgctctc cagcggtaaa attcgcgtaa ttggttcgac aacctatcag
     9121 gagttcagca acattttcga gaaagaccgt gctctggcgc gtcgcttcca gaaaattgat
     9181 attactgaac cgtcgatcga agaaactgtt caaatcatca atggcctgaa accgaagtat
     9241 gaagcgcacc acgacgtgcg ttataccgca aaagcggtgc gtgcggcggt agagctggcg
     9301 gtgaaataca ttaacgatcg tcatctgccg gataaagcca ttgatgttat cgacgaagcg
     9361 ggcgctcgcg cacgtctgat gccggtaagc aaacgcaaga aaaccgttaa tgtggcggat
     9421 attgagtccg tggtggcccg tattgcacgc attccagaga agagtgtttc tcagagtgac
     9481 cgcgataccc tgaaaaacct cggcgatcgc ctgaaaatgc tggtcttcgg ccaggataaa
     9541 gccattgagg cgctgactga agccattaag atggcgcgtg caggtttagg tcacgaacat
     9601 aaaccggtcg gttcgttcct gtttgccggt cctaccgggg tcgggaaaac agaggtgacg
     9661 gtacagcttt cgaaagcgtt gggcattgag cttctgcgct ttgatatgtc cgagtatatg
     9721 gaacgccata ccgtcagccg tctgattggt gcgcctccgg gatacgttgg ttttgatcag
     9781 ggcggtttgc tgaccgatgc ggtaatcaag catccacatg cggtgctgct gctggacgaa
     9841 atcgagaaag cgcatccgga cgtgttcaat attctgttgc aggtgatgga caacggtacg
     9901 ctgaccgata acaacggacg caaagcggac ttccgtaacg tggtgctggt gatgaccact
     9961 aacgccgggg tacgggaaac tgagcgcaaa tccattggtc ttatccacca ggataacagc
    10021 accgatgcga tggaagagat caagaagatc tttacaccgg aattccgtaa ccgtctcgac
    10081 aacattatct ggttcgatca tctgtcaact gacgtgatcc atcaggtggt ggataaattc
    10141 atcgtcgagt tgcaggttca gctggatcag aaaggtgttt ctctggaagt gagccaggaa
    10201 gcgcgtaact ggctggccga gaaaggttac gaccgggcaa tgggcgcacg tccgatggcg
    10261 cgtgtcatcc aggacaacct gaaaaaaccg ctcgccaacg aactgctgtt tggttcgctg
    10321 gtggacggcg gtcaggtgac ggctgcgctg gataaagaga aaaatgagct gacttacgga
    10381 ttccagagtg cacaaaagca caaggcggaa gcagcgcatt aatctgattg tcaggtaggt
    10441 tggtgaagtc cgtaatctcg tcaggggtta cggacttttt atttatgggg tggaacggtg
    10501 aaaaccctat ttttggaggt gaaggtaagt tgttgataat tagtgctgct ggaaggtaag
    10561 gataaaaaag ggtgctgcag gagaatggga tggttttgct ttattaacaa cgggctaaac
    10621 gtgtagtatt tgagttcact gccgtacagg cagcttagaa atcgacgggg tgcggtaaaa
    10681 cctttgcgaa cgcgttcact gccgtacagg cagcttagaa attcacaggt aacatactcc
    10741 acccgcccac catgttcact gccgtacaga cagataaaat gcgaaaaaaa agctcgcact
    10801 ttcgtacgag ctcttcttta aatatggcgg tgaggggggg attcgaaccc ccgatacgtt
    10861 gccgtataca cactttccag gcgtgctcct tcagccactc ggacacctca ccaaattgtt
    10921 ttgctaccaa acctcatggg tggcaacggg gcgttactat agggagttgg agtaaaacgg
    10981 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaact tgtgcagtat
    11041 atctacatcg agacaagtta tggacttata cttccaaagt acttcataca tatcacaaaa
    11101 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
    11161 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
    11221 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
    11281 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
    11341 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
    11401 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
    11461 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
    11521 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
    11581 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
    11641 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag caccgtggta
    11701 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
    11761 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
    11821 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
    11881 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
    11941 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
    12001 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
    12061 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
    12121 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
    12181 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
    12241 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
    12301 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
    12361 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
    12421 accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
    12481 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
    12541 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
    12601 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
    12661 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
    12721 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
    12781 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
    12841 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
    12901 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
    12961 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
    13021 gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
    13081 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
    13141 acgagtttgg gtatcaggga aggtgacctc cggtttttga tccgttaagt cagagatacg
    13201 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
    13261 ttcaaacgcg gctaacgcac aaaagacaaa cagggcaatt aatgcgccgg gttgagcatt
    13321 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
    13381 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
    13441 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
    13501 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
    13561 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
    13621 cagtaacata acgctgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
    13681 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
    13741 atgatcgagc gtatcaacat ccgccaccac gcggttgagc aattcgccct gacgatagcg
    13801 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
    13861 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
    13921 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
    13981 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
    14041 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
    14101 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
    14161 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
    14221 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
    14281 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
    14341 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
    14401 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
    14461 gtcgccaaca ggtgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
    14521 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
    14581 atcccgcaat tttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
    14641 ccatgattct ggtgataaat cacgtaattc tatcccgttg attcgtaatg atccctgata
    14701 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcggccaac
    14761 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
    14821 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc agcggatcgg tcaatgctaa
    14881 ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
    14941 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
    15001 gaaaaactct ggcgcaagga tcagggccag aaaacctgca gccagcgtca ccccagtatc
    15061 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
    15121 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gtcgtagcac
    15181 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
    15241 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
    15301 taagcgagca agagcgagaa agttacgccg gttagcatcg gcagccccca ttccaaccag
    15361 cgccataaac aacggaatta gcggtgctgt gcccagcaga atgagcgccg cagcccagtt
    15421 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
    15481 cagatagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
    15541 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
    15601 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
    15661 ccagaccacc catgcgcgca atacaaaggt cagaaccagt aacgtaaagg gaagcagcag
    15721 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
    15781 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
    15841 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
    15901 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
    15961 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
    16021 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
    16081 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
    16141 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
    16201 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
    16261 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
    16321 cgcagacgaa caccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
    16381 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
    16441 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
    16501 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
    16561 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
    16621 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
    16681 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
    16741 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
    16801 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
    16861 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
    16921 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
    16981 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
    17041 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
    17101 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
    17161 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
    17221 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
    17281 acagtattct tatatgcgca taaccatgta tgtaaatacc atgtttaccg tgctagtgaa
    17341 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
    17401 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
    17461 gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac
    17521 agagagacaa taataatggt agatagcaag aagcgctctg gcaaagatct cgaccgtatc
    17581 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
    17641 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
    17701 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
    17761 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
    17821 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
    17881 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
    17941 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
    18001 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
    18061 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
    18121 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
    18181 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
    18241 tgctgatcct tattgccctg tttgccgtct ggttgatggc tgccttacta agctttaacc
    18301 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
    18361 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
    18421 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
    18481 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
    18541 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
    18601 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
    18661 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacta
    18721 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
    18781 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
    18841 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgtggc gcgctagcgc
    18901 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
    18961 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
    19021 ctgccgaccc ggacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
    19081 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
    19141 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
    19201 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
    19261 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagtcacaat
    19321 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccgcagcagc
    19381 cgtattatgc acctgcagct gaacagcctg cgcaacagcc gtattatgcc cctgcgccag
    19441 aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccact tttgctccac
    19501 agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag ccgttgtacc
    19561 aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt gtagaagaga
    19621 caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag cgagcccgtg
    19681 aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa gaaccagaac
    19741 cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta gaagccgctg
    19801 ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg ggggctgccg
    19861 caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt ccgcaggtca
    19921 aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca actcgtcgtg
    19981 aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa aaagcccgtg
    20041 aagcccagcg caatcagtat gattctggcg atcagtataa cgatgatgaa atcgatgcga
    20101 tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc tatggcgaac
    20161 agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca gaggctgaac
    20221 tggctcgtca gtttgcgcaa actcaacaac aacgttattc cggcgaacaa ccggctgggg
    20281 cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg ctggatgatg
    20341 gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg caacaaccgg
    20401 ttg
//
LOCUS       DADLSB010000066        20374 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.066, whole genome shotgun sequence.
ACCESSION   DADLSB010000066 DADLSB010000000
VERSION     DADLSB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.066"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(178..2499)
                     /locus_tag="JN331_002272"
     CDS             complement(178..2499)
                     /locus_tag="JN331_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY7260404.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     GTERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLVPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2596..3108)
                     /gene="greB"
                     /locus_tag="JN331_002273"
     CDS             complement(2596..3108)
                     /gene="greB"
                     /locus_tag="JN331_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY7260405.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            3300..4019
                     /gene="ompR"
                     /locus_tag="JN331_002274"
     CDS             3300..4019
                     /gene="ompR"
                     /locus_tag="JN331_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY7260406.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            4016..5368
                     /gene="envZ"
                     /locus_tag="JN331_002275"
     CDS             4016..5368
                     /gene="envZ"
                     /locus_tag="JN331_002275"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY7260407.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5449..7071)
                     /gene="pckA"
                     /locus_tag="JN331_002276"
     CDS             complement(5449..7071)
                     /gene="pckA"
                     /locus_tag="JN331_002276"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY7260408.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7450..9174
                     /locus_tag="JN331_002277"
     CDS             7450..9174
                     /locus_tag="JN331_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY7260409.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWALWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9309..10187)
                     /gene="hslO"
                     /locus_tag="JN331_002278"
     CDS             complement(9309..10187)
                     /gene="hslO"
                     /locus_tag="JN331_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY7260410.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDSKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10212..10613)
                     /gene="hslR"
                     /locus_tag="JN331_002279"
     CDS             complement(10212..10613)
                     /gene="hslR"
                     /locus_tag="JN331_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY7260411.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10624..11292)
                     /gene="yrfG"
                     /locus_tag="JN331_002280"
     CDS             complement(10624..11292)
                     /gene="yrfG"
                     /locus_tag="JN331_002280"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY7260412.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11357..13492)
                     /gene="igaA"
                     /locus_tag="JN331_002281"
     CDS             complement(11357..13492)
                     /gene="igaA"
                     /locus_tag="JN331_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY7260413.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPSPTGASAAPLSLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATGVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13812..14372
                     /gene="nudE"
                     /locus_tag="JN331_002282"
     CDS             13812..14372
                     /gene="nudE"
                     /locus_tag="JN331_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY7260414.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14537..17089)
                     /gene="mrcA"
                     /locus_tag="JN331_002283"
     CDS             complement(14537..17089)
                     /gene="mrcA"
                     /locus_tag="JN331_002283"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY7260415.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITHKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLGQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17209..17988
                     /gene="hofM"
                     /locus_tag="JN331_002284"
     CDS             17209..17988
                     /gene="hofM"
                     /locus_tag="JN331_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY7260416.1"
                     /translation="MAFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            17988..18527
                     /locus_tag="JN331_002285"
     CDS             17988..18527
                     /locus_tag="JN331_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY7260417.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18511..18951
                     /gene="hofO"
                     /locus_tag="JN331_002286"
     CDS             18511..18951
                     /gene="hofO"
                     /locus_tag="JN331_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY7260418.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18941..19345
                     /gene="hofP"
                     /locus_tag="JN331_002287"
     CDS             18941..19345
                     /gene="hofP"
                     /locus_tag="JN331_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY7260419.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNYVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19257..>20374
                     /gene="hofQ"
                     /locus_tag="JN331_002288"
     CDS             19257..>20374
                     /gene="hofQ"
                     /locus_tag="JN331_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY7260420.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRDLGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQ"
BASE COUNT         4614 a         5284 c         5538 g         4938 t
ORIGIN      
        1 ttaattaagg tttcagttgt taatgaagtg ataaatatta acggagtata ttgtgagttt
       61 ggctgtggaa taaaagtgcc ggagatattc tccggcactt ttatgtcagg caaagcgttc
      121 gttgccagat gcggcgtgaa cgccttatcg cgcaggttta ggggccagtc cgggcgttta
      181 acgtttttta cccatcgctg ccgccagcgc atccatcatc gcgctattac cggcaggctg
      241 cgcttcacga ccgcgtggtt tggctgccgg gcggttgttt tgcgggcgtt cattaccgcc
      301 gccgcgacga gagttggttt cgccaggctg ctcgtccaga cgcatagtca gggcgatacg
      361 tttacgctga agatccactt ccagcacttt caccttcaca atgtcgcccg ctttcaccac
      421 ggtatgcgga tcttccacaa acttgttcga caatgaagag atgtgaacca ggccgtcctg
      481 atgcacgcca atatcgacaa acgcgccaaa gttggtgacg ttggtcactg cgccttcgag
      541 gatcataccc ggttgcaggt cgttcattgt ctcgacgcca tcggcaaact gagcggtttt
      601 aaattccgga cgcggatcgc gacccggttt ttccagctct ttgatgatgt cagttactgt
      661 cggcacaccg aatttttcat cagtaaagtc agacgctttc aggttacgca gttcgctgct
      721 gttacccatc agatctttca gtgcctgctg tgttgctgcc agaatgcgtt ccaccaccgg
      781 ataggcttcc ggatgaacgg tagaggcgtc cagcgggtta tcaccgtggt taatgcgcag
      841 gaagcccgcg cactgctcga aggctttcgg caccagacgg ctgactttca gcagttgctg
      901 acggttctgg aactggccgt tctcatcgcg ccaggccacg atgttttgcg ccatcatgcg
      961 cgtcaggccc gccacgcggg ttaatagcgg aacagaagcg gtgttgagat cgacgccaac
     1021 ggcgtttacg cagtcttcta ctactgcatc cagtttgcgg gccagttgcg tctggctgac
     1081 gtcatgctga tactgaccta cgccgataga tttcggatcg attttcacca gctccgccag
     1141 cggatcctgc aaacggcggg cgatagatac cgcaccacgc agcgaaacgt cgagatccgg
     1201 gaactcctgc gctgccagct cggaagctga gtaaaccgac gcgccagctt cgctgacgat
     1261 cactttctgt gcggtcactt tcgggaactg cttctgcacg tcgagataga aacgttcagt
     1321 tccgcgggaa gctgtaccgt tgccgatcgc caccagttca acgttatgtt tttcacacaa
     1381 ggcagcaacg gtcatcgctg cttttgcggc ttgtccggtg tgcgggtaaa tggtatcggt
     1441 cgccaccagt ttgccagtgg catcgaccac cgccactttt accccggtac gcagacccgg
     1501 atcgaggccc atcgttgcac gcagtccggc aggggccgcc atcagcagat cgtgcaggtt
     1561 acgggcaaag acgttgattg cttcatcttc cgcgcgttcg cgcacggtac ccatcagttc
     1621 ggtttccaga tgcatcagca ccttgatgcg ccaggtccag ctcactacgc ctttgcgcca
     1681 gctatccgcc ggggcattgt tcaggcgcag gccaaggtga tccatgatga tttgctcgca
     1741 atagctctct ttgggcggct catcgaactg cggatcggca ttcagcgaaa gctggagtac
     1801 gccttcgtta cgcccacgga acatcgccag cgcgcggtga gaaggcaccg tggacaacgg
     1861 ttcgtgatga tcgaaatagt cgcggaattt cgccccttcc tcttctttac cgctcaccac
     1921 cgtagaaacc aaatgcgcgt tcttccacag ataatcacgc actttcgcca gcagcgcggc
     1981 atcttcggca aaccgttcca tcaggatata gcgcgcgcca tccagcgcgg ctttggtatc
     2041 tgccacgcct ttatcggcat caacatattg tgcagcggcg acttctggcg tgtgtgacgg
     2101 atcgctccac agcaggtcag ccaacggctc aagccctgct tcaatggcga tttgcccgcg
     2161 ggtgcggcgt ttaggtttgt agggcaggta gaggtcttcg agttcggttt tgcttagggt
     2221 ggcgttgatg gccttcgcca gatcatcggt gagtttgcct tgctcggaaa tggatttgag
     2281 gatcgcctga cgtctctctt ccagctcgcg cagatagctc agacgcgttt ccagattacg
     2341 cagctgcgtg tcatccagac cgccggtgat ttccttacga taacgtgcga taaacggcac
     2401 ggtattccct tcgtcaagca ggcgaacggc agcgtcaacc tgttccgggc gcgcctgaat
     2461 ttcacccgca ataatgcggc agaacgaatc attcatcatg gtttgagttc atcttttcgg
     2521 atcaaaaatc aggggatagt tatacggact ggctggcaaa aatgccagcc atcggcagga
     2581 ggttaagact cttccttacg gtttcacgta ctcgatagca ttaacatacc agttcgcttc
     2641 cccggcaggg gtattcacca ccgccagatc gccgacttct tttttcaaca atgcgcgggc
     2701 catcggggaa tcgatagaga tgtaatcttt acggccaaaa atttcatcgt agccgacaat
     2761 acggaaacgg tgagtcacgc catcgtcgtt ttcaatctcc acccacgcgc caaaaaagac
     2821 tttgccttcc tgctgagggg agtaatcgac gattttgaga ttttccaggc atttagtgag
     2881 atagcgcacg cgacggtcga tttcacgcag acgcttttta ttatactgat agtcagcatt
     2941 ttcgctgcgg tcgcccagac ttgcggccca ggtcaccttt tttgtgacct ccgggcgttc
     3001 ttcacgccag agataattaa gctcttgttt gagtttttca tacccttccc gggtaaccag
     3061 gggcgttttc atctcgttga ttccctttgt ctgtttgata atgcgcacat tgggtataac
     3121 gtgatcatat caacagaatc aataatgttt cgccgaataa attgtatact taagctgctg
     3181 tttaatatgc tttgtaacaa tttaggctga aattcatacc agatttagct ggtgacgaac
     3241 gtgagctttt ttaagaatac acgcttacaa attgttgcga acctttggga gtacaaacaa
     3301 tgcaagagaa ctacaagatt ctggtggtcg atgacgacat gcgcctgcgt gcgctgctgg
     3361 aacgttatct caccgaacaa ggcttccagg ttcgaagcgt cgctaatgca gaacagatgg
     3421 atcgcctgct gactcgtgaa tctttccatc ttatggtact ggatttaatg ttacctggtg
     3481 aagatggctt gtcgatttgc cgacgtcttc gtagtcagag caacccgatg ccgatcatta
     3541 tggtgacggc gaaaggggaa gaagtggacc gtatcgtagg cctggagatt ggcgctgacg
     3601 actacattcc aaaaccgttt aacccgcgtg aactgctggc ccgtattcgt gcggtgctgc
     3661 gtcgtcaggc gaacgaactg ccaggcgcac cgtcacagga agaggcggta attgctttcg
     3721 gtaagttcaa acttaacctc ggtacgcgcg aaatgttccg cgaagatgag ccgatgccgc
     3781 tcaccagcgg tgagtttgcg gtactgaagg cactggtcag ccatccgcgt gagccgctct
     3841 cccgcgataa gctgatgaac cttgcccgtg gtcgtgaata ttccgcaatg gaacgctcca
     3901 tcgacgtgca gatttcgcgt ctgcgccgca tggtagaaga agatccagcg catccgcgtt
     3961 acattcagac cgtttggggc ctgggctacg tctttgtacc ggacggctct aaagcatgag
     4021 gcgattgcgc ttctcgccac gaagttcatt tgcccgtacg ttattgctca tcgtcacctt
     4081 gctgttcgcc agcctggtga cgacttatct ggtggtgctg aacttcgcga ttttgccgag
     4141 cctccagcag tttaataaag tcctcgcgta cgaagtgcgt atgttgatga ccgacaaact
     4201 gcaactggag gacggcacgc agttggttgt gcctcccgct ttccgtcggg agatctaccg
     4261 tgagctgggg atctctctct actccaacga ggctgccgaa gaggcaggtc tgcgttgggc
     4321 gcaacactat gaattcttaa gccatcagat ggcacagcaa ctgggcggcc cgacggaagt
     4381 gcgcgttgag gtcaacaaaa gttcgcctgt cgtctggctg aaaacctggc tgtcgcccaa
     4441 tatctgggta cgcgtgccgc tgaccgaaat tcatcagggc gatttctctc cgctgttccg
     4501 ctatacgctg gcgattatgc tattggcgat aggcggggcg tggctgttta ttcgtatcca
     4561 gaaccgaccg ttggtcgatc tcgaacacgc agccttgcag gttggtaaag ggattattcc
     4621 gccgccgctg cgtgaatatg gcgcttcgga ggtgcgttcc gttacccgtg cctttaacca
     4681 tatggcggct ggtgttaagc aactggcgga tgaccgcacg ctgctgatgg cgggggtaag
     4741 tcacgacttg cgcacgccgc tgacgcgtat tcgcctggcg actgagatga tgagcgagca
     4801 ggatggctat ctggcagaat cgatcaataa agatatcgaa gagtgcaacg ccatcattga
     4861 gcagtttatc gactacctgc gcaccgggca ggagatgccg atggaaatgg cggatcttaa
     4921 tgcagtgctc ggtgaggtga ttgctgccga aagtggctat gagcgggaaa ttgaaaccgc
     4981 gctttacccc ggcagcattg aagtgaaaat gcacccgctg tcgatcaaac gcgcggtggc
     5041 gaatatggtg gtcaacgccg cccgttatgg caatggctgg atcaaagtca gcagcggaac
     5101 ggagccgaat cgcgcctggt tccaggtgga agatgacggt ccgggaattg cgccggaaca
     5161 acgtaagcac ctgttccagc cgtttgtccg cggcgacagt gcgcgcacca ttagcggcac
     5221 gggattaggg ctggcaattg tgcagcgtat cgtggataac cataacggga tgctggagct
     5281 tggcaccagc gagcggggcg ggctttccat tcgcgcctgg ctgccagtgc cggtaacgcg
     5341 ggcgcagggc acgacaaaag aagggtaaat aaacgggagg cgaaggtgcc tcccgtttcg
     5401 ttttgctttc tataagatac tggatagata ttctccagct tcaaatcatt acagtttcgg
     5461 accagccgct accagcgcgg cacccgcagg ggtgtcggtg tatttatcga agttgtcgat
     5521 aaacagtttc gccagggttt cggctttttc ctgccactgt tccggagaag cgtaggtgtt
     5581 acgcggatcg agaatcttcg tgtctacgcc cggcagttcg gttgggatcg ccaggttaaa
     5641 catcggcaga gtgaaggttt ctgcattatc cagcgaaccg ttgaggatgg cgtcgataat
     5701 ggcgcgggta tctttaatcg agatacgttt gccagtgccg ttccagccag tgttaaccag
     5761 ataagcctgc gcgcccgccg cctgcatacg tttcaccagc acttctgcgt actgagtcgg
     5821 gtgcagcgac aggaacgccg cgccgaagca agcggagaag gttggcgtcg gttcggtgat
     5881 gccacgctca gtaccggcca gtttggcggt gaagccagag aggaagtgat actgggtttg
     5941 atcggcagtc aggcgagaaa ccggcggcaa cacgccgaaa gcatcagcag tcaggaagat
     6001 aaccttagtc gcgtggcccg ctttggaaac cggcttaacg atgttatcga tgtgatagat
     6061 tggataagaa acgcgggtgt tctcggtttt tgaaccatca tcaaagtcga tagtgccatc
     6121 ttcacgcacg gtgacgtttt ccagcagtgc atcacgacgg atagcgttgt agatttcagg
     6181 ttccgcttct ttcgacagct tgatagtttt tgcgtagcag ccgccttcga aattaaacac
     6241 gccgtcatcg tcccagccgt gttcgtcatc gccaatcagg cggcgtttcg ggtcggtgga
     6301 aagggtggtt ttaccggtgc cggaaaggcc gaagaacacc gcaacatcgc ctttctcgcc
     6361 aacgttggcg gagcagtgca tagaagcgat acctttcagc ggcagcaggt agttcatcat
     6421 cgagaacatc cctttcttca tttcgccgcc gtaccaggtg ccgccaatca gctgcatgcg
     6481 ctcggtcagg ttaaacgcca cgaagttttc ggagttcaga ccctgttctt tccactgcgg
     6541 gttagtgcac ttcgcgccgt tcataacgat aaagtctggt ttgaaacctg ccagctcttc
     6601 atcgctcggg cgaataaaca tgtttttgac aaaatgcgcc tgccaggcca cttcggtgat
     6661 gaaacggacg gaaagacgag tatccgggtt cgcaccacag aaagcgtcga caacgaacag
     6721 acgtttgccg gaaagctgcc tggtcaccag gcctttcaga tgctgccagg tttccggaga
     6781 gagaggtttg ttgtcgttct tacctttgcc tttgtctgcc caccagaaag tatcgcgagt
     6841 ggtatcgtca cggacgatat acttatcttt tggtgaacga ccggtgaaga tcccggtatc
     6901 gacggcaacg gcacccagat tagttaacac cccgcgctca taacctgtca ggctcggatc
     6961 gagctcttcc tgatacagca ggtcgtagct tgggttgtaa acgatatcat gtacgtcact
     7021 gataccataa gcctcgagtt cttgcggggt caaaccattg ttaacgcgca tttcactgct
     7081 ccttagccaa tatgtattgc ctgaatagta aagtcttttt gggggtgtta accgcgacaa
     7141 ggctcataga tttacgtatc tggagaaatt catatttgac aaaaaactcc gtgattcctg
     7201 tcacgaaacg gttgctatta tcgcagaaat agcattttag gtgtggaaaa tgttctcaaa
     7261 aggttaaatc ttggtttata tggcgggaat attgtgagtg gaatcgcatt cctgaatgaa
     7321 attgatgcaa attcaaccgg taaagtatcg attcaccacg ctaacttttt ttgattattt
     7381 tctgaaaaaa gcaaaatact actgcccgga tgcgataatt aacccacctt ttgattctgg
     7441 ataaccaaca tggataacgt tgaactttca ccggcaacac gctgggggat gattgctacc
     7501 ggattacttc aggggctggt ttgctacctg cttatcgcct ggcttgccgg aaaaaatcac
     7561 agttggattg tttatggcgt gcctgcgacc gtcgcgtttt catccgtttt actcttctcc
     7621 gtgatctctt ttaaacagaa gcgcctttgg ggatggctgg cgctggtgtt tattgccacg
     7681 acagggatga gcggttggct gaagtggcaa actgatggca tgaacccctg gagagctgaa
     7741 aaggcgattt gggattttgg ctgctatctg ctgttgatgg caatgttgtt gctaccgtgg
     7801 atacaacaaa gccttcgtat ccgcaatggc agtagccgct atagctactt ttaccagtca
     7861 gtatggcata acgtacttat attactggtg atttttctct ccaatggcct gacgtggctg
     7921 gtgcttttac tgtggggtga gttgtttaaa cttgttggca tcacgttttt caatacgctc
     7981 ttttttgcaa ccgactggtt tatgtatctt acatttggtc tggttaccgc actggcggtg
     8041 atcctcgcgc gtacacagtc acgtttaatc gactctattc aaaagttgtt cacgctaatc
     8101 gccacggggt tgctgccgtt agtatcattg ctaaccctga tgtttatcat caccctgccg
     8161 tttacgggcc tgagcgcgat ttctcgccac atctccgccg ccgggttgct gttaacgctg
     8221 gcctttttgc aattgatctt aatggctatt gtccgcgatc cgcaaaaagc gtcacttccc
     8281 tggacggggc cgttgcgttg cctgattaaa accgctttgc tggttgctcc gctgtatgtg
     8341 ttcgtcgcca cctgggcgtt atggctgcgg gtcgctcagt acggctggac tgtcgaccgc
     8401 ttgcagggcg cgctggcgga gctggtgtta ctggtgtggt cgctggggta ttttgtcagt
     8461 atcgtctggc gtaatgggca aaatcccctt gttcttcagg gcaaagtgaa ccttgcggtt
     8521 tcgttattgg tgttggtgat actggtgctt cttaattcgc cggtgctgga cagtatgcgt
     8581 attagcgtga acagtcatat ggcgcgttat cagagcggca aaaacacgcc agaccaggta
     8641 actatctaca tgctcgaaca gagcggtcgc tatggacgtg cggcgcttga gtcgctgaaa
     8701 agcgatgccg ggtttatgaa agacccgaaa cgcgcgcggg atctgctgat ggcgttagat
     8761 ggagagcaac atcttcagga acaggtatcg gaaaaagtat tagccgaaaa tgtgttaatt
     8821 gcccctggtt ctgttaaacc ggatgcgaca ttctggtcgg ccttaatcca ggatcgctat
     8881 aacgtgatga cctgtattga aaaagacgcc tgcgtactgg tcgagcaaga tctgaatagt
     8941 gatggtcagg cggagcggat cctgtttgct tttaatgatg acagagtcat tgtctatggc
     9001 tttgactcag acagaaaaga atgggacgcg cttgatatga gtttacttcc gaacgaaata
     9061 acgaaagaaa aattactcac agctgcgaag gatggaaaac tggggacgag gccgaaagcg
     9121 tggcgcgatc tgacggtaga tggtgaaacg ctggaaataa acttaagtaa gtgacaacgc
     9181 ttttatcgga tacgacgctg tatctgacca aaataatttg cgatttattt cataggcgct
     9241 ggtaagcgca gcacatcagg ccattaagtc gtaaaatccg gcagagccct ctctgccgga
     9301 catactcatt aatgaacttg cggatctgcc ggagacgcgt tgttgcggat ttcagcaata
     9361 tccatcgcat tgaacagata gtggttaccg cagtaatcac aatgcatgtc aatttcgcca
     9421 tcttccgcca ggatgctatc aacttcttca tcaggcagcg ttttcagcgc atcggcgcaa
     9481 cgttcacgcg agcaggtgca tttgaactcc acatcctgcg gatcgtaaac cgtcacctct
     9541 tcttcgtgat acaaacgcca caacacttcg tttgccggta aggtcagcag ttcttcggtt
     9601 ttgatggttt cggttagcgt cgccaggtgg tcaaagtcgt cctgctgggt attttgcgca
     9661 ggcattacct gcaacaacat accgcctgca gccggtttgc tgtctacgtc gccggtgcga
     9721 ataaacaggc gcgtcggcag ctgttcagaa cgcataaagt aatcttccag gcaggccgcc
     9781 agggtatcac cttccagacc aactacgccc tgatagcgtt cgccttcgct cggggtaatg
     9841 gtgatcacca cgtaaccatt gccgaccagc gttttcaggt cggcattttc tggaatttcg
     9901 ccctgcacgc gcgccacacc gcgcatttgc tggttattgt taccgttaat aaccgccaga
     9961 ttcatcggac cgtcgccctg cagctgtacg gtgatatcac catcaaactt cagcgtagcg
    10021 gttaacaggc tggtcgcaac cagcagttct gccagcacgt ttttaacggg ctgcggataa
    10081 tcgtggttct caaggatctg ttgcagggtt tccgaaacgg ttaccagttc gccgcgaacg
    10141 gcaaaatttt caaacagata gcgatgtaat tggtcatgtt gcggcataat catctctctt
    10201 gcaggtgaca gttattcact gtcgccgtgt ttaaatcgta acaggtcgcg gcgctctttt
    10261 ttgtccgggc gtcggtccgg gtgcggcatg gttaaggcat taagtttacg tgccatcgcc
    10321 attttttcgc gtttctctac actttccgca gtctcttcat acagcaaggc tgcctcgctg
    10381 gcggggcgac gctgttcagt aatcgccttt acaatcaccg tgcgttcgtc atttccctgg
    10441 cgcagagtga gcgtggcatt cagctcgacg attttgctcg gcttgctgcg ctgcccgttg
    10501 taatgcacct taccgccttc aatcatttca cgggccagcg cgcgggtttt ataaaaacgg
    10561 gcagcccata gccatttatc cagtcgaacc tcaacagcag gtttctcttt catggcgtct
    10621 ccttcacatt agcgagggga tcaggcggcg gtagtcattc agtgacggat ggcgttgata
    10681 ctgtttctcg gcaatcccgg aatcaggatt agtcacgccg aggcagtaac gaataccaaa
    10741 ttgcgcggca gcatcgagaa tcgcttcgct gtcatcaata aacagcgttc tttcagcttt
    10801 cagacccgta gcttcggcca ccgcatgcca taaccgctga tcctctttcg gataaccaaa
    10861 tgtgtgggtg gaaagtaata aatcaaggtg tgcgtccaga cccgtatgct caagttttac
    10921 cgccaggttg tgcggatgag cattggtgag caaaattcgc tgcttaccgc tggctttcag
    10981 tgcctcaaga aacggaatgg tatcttcacg cagtacggcg cgcggtccca tctcggtggt
    11041 catcgcacag atatccagac ccagttgctc actccagtaa tcaagacagt accagtttag
    11101 cgtatgctgt acgtcgtgat attgctggcg catatattcc atcgcttcct gtggcgtaac
    11161 cccgtttttc gcgccccatg tttcaggcac cagcttttgc cagaaatagt tatcgaaggc
    11221 gaggtcgagc aacgtgccgt ccatatccag cagaacggta tctacgtcct gccaggcaat
    11281 gttgatatgc atgagggaaa tctccagagt gaagcaattt gcgcgacagg gtagcataac
    11341 ctgccgcgca aacgtgttat tcgataaggc tttctgaagg ggtgatcagt tgcgggttca
    11401 ggcagctttc ataataggcc tgaatctcca tcatgcgagt gcgatgacgc tggtaacgac
    11461 gccaggcctg tacgccattg taaatggcgc tacccagcat cgtcagtagc agcaatgtgg
    11521 tgctgagata gcgccacagg ccggaacgat ccgggatcgg atgaaggcca atatgctgcg
    11581 taccattggc gtcagtgaag atttttgtga cgataccttc ggcgttaaac ggcgtatgca
    11641 tcagcatttg tgccagtttc tggaaagcgt tccactgttc ttgcggcggg tagtcgtaaa
    11701 gtgatgccga aggccagggc tgatcaacaa aatcgctgcc ttcgtcgctg acaatcagga
    11761 atccgccggg ggccggactg tttagtgatt gtgccgctcg cgccgtttca tgcgtgataa
    11821 acggcgcggt ggaggttgcc accaggttat ccagcgattc cgcactcacc gggcgtaata
    11881 acacattcac gccatcgagc ttcccggcgt tggcgcgttt taccagcgcg tcccagtctt
    11941 tactgttgcc gagattgacc agcgcatttt tcagtcgcac acagtcatct ttggcagaac
    12001 ataaatccgc tgtcttcagt acaatgtcgc caaaatcatc aagcaatacc atgccggatt
    12061 tttgaatcgc tgaacgtaat gaggcactga cgcgagattc atcttccggt ttagggtgca
    12121 gctggcgatt aactgcttca gtcaatgccg tcgctttgtt gaccagttca gattctggta
    12181 atggtaatga gcgggcgtca ttccagatga tctgcgagca gtcaaacggt aaaaaaggtg
    12241 aattggtttt cgcgctccag gttccggaag tgcgaatatt acacattccc gtaccgctaa
    12301 tacgcaatgt atcgcctacc cgcacgccag cgtcagccag ttgttttacg ccggtggctt
    12361 caatggtctg cgcgcctttc atccatgaga gagtgaattt cagcggcata tccagcggga
    12421 tccagaataa cagcataaac agcaccagca gcgagcccgc agcgataatc gtactgcgca
    12481 gccagtgctg taacggaaag ttttttactt catcatgcag cgaaagatat cgcccctgac
    12541 gcactacgtg gcggtcgaga tagatatcaa tatcggtttg ttgaccgaga tcctgagcaa
    12601 tgtatggctg ccagtgtgcg ggatagacca ggtcgataat accgagtgaa atattgttga
    12661 tctgttcctg atcgttttcg ccaaacaatc cccaacgacg gggtgtaccg cgcagacaat
    12721 gaatttctcg cagggaggat tttgccgggg gcgcgaatag cccccacaga cctgcgccca
    12781 gcagcagtaa tgcgccgcct gccagccacg gaacaaatac atccggggta atcaggcaga
    12841 aaaagaacat caggaaggag gcgacgatca gcaacgcttc acgcagcccg cgcggacgac
    12901 tcagcgcata ttcttcatgc gtttctttgc gaatattgag tagctcgatt tgctcactct
    12961 cttccccgcg aatcgacgcc tgcgtggacg gaacaggttg caaggcatag ccgcgcgttt
    13021 cctgcatgta ctcctgcagc gtatgaccgt tgagtgaaat aaccaacggc agcgaatcgg
    13081 tatgaatcag ttcaacggta ttctcatcgt tgatgtactg ttcccagaag gggggcaggt
    13141 ggacttctac cgaatcgagg tagtaacgcc atttattcgg atcgtcggta gagatgccgt
    13201 aacgtgtgat agcgtgtgtc agcatcatga cgttgttgct ttcggcattc agcgccagag
    13261 agagcggtgc cgcgctagct cccgttgggc taggcacctg taatacctgc gtcaggctct
    13321 caagataatt ttcaacggcg ctacgttctt caggtgtgag tttacgcgtt tgcgcatccg
    13381 cgaaggcgtt ggtccagggc agctgacgcc gtctggatcg cacttttatc agccatcccg
    13441 caagtagtga gcaggccagc aaagcagcta aaaaaatcac aatggtgctc atgctttccc
    13501 catcttactt agtctgtcag gcgtggtcag tcgcaccgga cacaatacat gattctgcgg
    13561 ttggctatcg gcaagcgtgg agtttaactt gagcatcatt aagcacatca cagtaaccgc
    13621 tgatcatagc aaaccctctg aaggcggaat atcagcaaat tcctggagtt atttcaacct
    13681 attgactttt aatttgattt gaggacgatt atttattaag ttacataaat gtaaataaca
    13741 tgcaattcac attgccgaaa gcgtgtccga tatcgcacaa taaccgctga tttcacagca
    13801 tagatcccac gatgagcaaa tcattacaaa aacccaccat tctgaatgtt gaaactgtag
    13861 cccgttcccg actgtttacc gtcgagagcg tggatctgga gttcagcaat ggcgtgcggc
    13921 gtgtttatga acgaatgcgt ccaaccaacc gggaagcagt gatgattgtg ccgattgtgg
    13981 acgatcacct gatcctgatc cgcgaatacg cagtgggaac tgaatcctac gaattaggtt
    14041 tttcgaaagg attaattgat ccgggtgaaa gcgtctacga agccgctaac cgcgagctaa
    14101 aagaagaggt tggatttggt gcgaacgatc tgactttttt gaagaagctc agcatggcac
    14161 cgtcttactt ttccagcaaa atgaatatcg tggtcgcgca agatctctac ccggaatcgc
    14221 tggaaggcga tgagccagag ccgctaccac aggtgcgctg gccgctggcg catatgatgg
    14281 atttgctgga agaccctgac ttcaacgaag cgcgtaatgt cagcgcgctg ttcctcgtgc
    14341 gcgaatggtt gaaagggcag gggcgagtgt aaatgcgtga agtttgcctg atgcacaacg
    14401 cttatcaggc ttatggggtt cctgcaatat attgattttt cacgattttg ttagccggat
    14461 aacgcgttca cccgcatccg gcataaacaa atgcactttg tcagccaact gaaaaggcgc
    14521 cgaagcgcct ttttaatcag aacaattcct gtgcctcacc attatcgata atggtcgttc
    14581 ccacctcgtg caccgcctgt tgtgtcggct gcgtaccttc gatgaaatac tcttcgcggc
    14641 tgttgccacc attagctaac tgcccggtgc tgcgatcgat attcaccgtc acaatacccg
    14701 gaggcggcgt cagcggctgc tccggcacac cttcaagaac ggctttcata taagcgtccc
    14761 atgcaggctg ggcactcttg gcaccgcctt cgtaacctga gatctgatct ttaatcgctc
    14821 cggaagccgt tgtatgaccg agattacgac ggtgatcatc aaagccaatc cagaccgagg
    14881 tcacaacgcc cggaccgtaa cccgagaacc acgcatcttt cgaactgtta gtggtaccgg
    14941 ttttcccgcc gatatcgcga cgctgcaaat cacgacctgc acgccagccg gtaccctgcc
    15001 agcctggttc accaaagata ttggtgttca aagcactctt aatcaggaat gccagcggag
    15061 tgttgatgac gtgcggtgcg tactcctgcg cgccagtctt cgccactaac gcctgatttg
    15121 cctgccccag ctgcggcatt ggtacagaaa cattctgctg ctcgcgggag atagcgacat
    15181 cttcaacatc gttattttcc agcacgttcg atttctgcgt atcaccgtaa atcactggaa
    15241 tatcgcattc cgggcaggct actttcggtt tcgcttcgaa aatcacgccg ccctgatcgt
    15301 tttcaatttt gctgataaac cacgggtcca ccaggaagcc gccgttcgcc atgaccgcgt
    15361 agccgcgcgc cacctgcatt ggggtgaagg acgctgaacc cagcgccagc gattcggtgt
    15421 ggacaatgtt ttgcgccggg aagccgaagc gttgcagata ttctgcagcg tagtcgacgc
    15481 ccatcgcccg cattgcacgt accatcacca cgtttttcga ctgacccagc ccctgacgta
    15541 agcgaattgg accagcatac tgcggtggtg agttcttcgg ctgccagtca gaaccggcac
    15601 ctgcatccca gcgagaaatt ggcacatcgt tcaacatact tgccagcgtc agacctttat
    15661 ccatcgccgc ggtgtagagg aacggtttga tgttggaacc cacctgacgc agtgcctggg
    15721 tggcgcggtt aaacttgctc tgattgaaat caaagccacc gaccagcgcc ataacggcac
    15781 cattttgcgg attgatcgac accagcgccg agttcacttc cggcacttgt gccagccacc
    15841 atgcatcgcc aacctgacga acccagattt gctgacccgt ttgcagaaca tcggtcactt
    15901 tacgcggcgt cggtccctgc tgagtatccg aacggtaagg acgcgcccag cgaacgcctt
    15961 ccatactcaa tgcgacggtc gacccgtccg ccagcatcgc cgtcgctccc tgaggattgg
    16021 cgctggtgac tgcggcaggc agcagcggac cataggtcgg cagcgccttc agcgtatcgg
    16081 taatcttgtt gttatcccac gccgactcgc ccactttcca cagcacattt gccgggccgc
    16141 gatagccgtg gcgcatgtcg tagtccagca cgttattacg taccgcctgc tgcgcggcct
    16201 gctgcacttt gtgggtgatg gtggtgtaaa tgcgataacc gtcttcatag gcactttcgc
    16261 cataacggtt atacatctcc tggcgcacca tttcgctcag gtacggcgca gagaaagcaa
    16321 tctccggcgc gtgataatta gcgttaatcg cctcagtgcg tgtctgatcg aactgttgct
    16381 gggtgatata cccttcatcc agcatccgcg acagtacgac gttacgccgc gcgacggcac
    16441 gatccattga gtagagcgga ttaaaggtgg aaggcgcttt cggcaacccg gctatcaccg
    16501 ccatttcgtt cagcgtcagt tggtcgaccg ttttaccgaa atagacttgt gccgcagcac
    16561 cgacaccata ggcgcggtaa ccaaggtaaa tcttgttcag ataaagctcg aggatttcgt
    16621 ctttcgtcag caactgttca atgcgaatcg cgaggaagac ttccttaatc ttacgcatca
    16681 gcgtgcgttc tggactgagg aagaaatttc tcgccagctg ctgggtaatg gtactcgccc
    16741 cttgtgacgc gtggccggag aacagcgcca cacttgccgc acggaagatc cccaccgggt
    16801 caacgccgtg atgctcgtag aagcggctgt cttccgtcgc gataaaggct ttcaccatct
    16861 ccggtgggat ttgatccaac gtaaccggaa tacgacgttt ctcaccgtat tgagcaatca
    16921 gctcgccatc ggcgctgtaa atctgcatcg gaatttgcag gcgaacatct tttaatgtcg
    16981 ccacatccgg cagttgtggc tcgatgtagc ggtataggcc ataaatcgag cctgctccca
    17041 gcagaatgca acagactgca aggatcaaaa aatactttac gaacttcact ggaaatttcc
    17101 catttagttt catttgggca gtttataaac aaacgcgcgg tagtataaag gcaagccagg
    17161 cgcattgata tacccgtcag agttacgggt gataaggaga tcatcacaat ggcatttaag
    17221 atctggcaaa ttggtttgca tttacaacag caagaagcgg cagcggttgc gatcgtgcgg
    17281 ggcgcaaaag aatgcttttt gcaacgctgg tggcggttgc cgctggcgca cgacattatc
    17341 aaagatgggc ggattgttga tgcgcagcag ctggctaaaa cgttgttacc gtggagtcgc
    17401 gaactgccgc agcgtcatca cattatgttg gcgtttcccg ccagtcgcac attacagcgg
    17461 tcatttccgc gcccgtcgat gtcccttggt gagcgggagc aaacggcttg gctgtcaggg
    17521 acgatggccc gcgagctgga tatggatccg gactccctgc gcttcgatta cagcgaagac
    17581 tcactcagcc ccgcttataa cgtgactgcc gcgcaaagca aagagctggc aacgctgctt
    17641 acgctggcag aaaggttgcg tgttcatgtt agtgcgatca ccccggatgc cagtgcatta
    17701 cagcgattcc tgcctttttt accttctcat cagcaatgtc tggcctggcg tgataacgaa
    17761 cagtggctgt gggcgacgcg ctatagctgg gggcgcaaac tggcggtggg gatgactagc
    17821 gcgaaggagc tggcggcagc gttatccgtt gatcccgaca gcgtcgcgat atgtggcgaa
    17881 ggcggatttg acccctggga ggccgtttct gttcgtcagc cgccgctacc gccgcctggt
    17941 ggagactttg ccatcgcgct ggggctggcg cttgggaagg cgtactgatg aacccgccaa
    18001 ttaatttttt gccctggcga cagcaacgcc ggaccgcttt tctgcgtttc tggttgctga
    18061 tgttcgttgc gcctctgctg ctggccgtcg ggataacgct aatactgcgt ctgacaggca
    18121 gcgccgaagc gcgcatagac gcagttttgc ttcaggcgga acaacaactc gcccgcagct
    18181 tacagataac gaagccacgt ttgctggagc aacagcagtt acgcgaacag cgttcgcaac
    18241 ggcagcgcca gcgacaattt acccgcgact ggcaatctgc gctggaagca ctggcggcgc
    18301 ttttacccga gcacgcctgg ctgacaacga taagctggca gcagggaacg ctggagatca
    18361 aggggctgac aacaagcatt accgcgttaa acgcactaga aacgtcactc cgccaggatg
    18421 cgtcttttca tctcaatcag cggggagcca cgcagcagga tgcgcaggga cgctggcaat
    18481 ttgagtatca gttaacaagg aaggttagcg atgaacatgt tctttgactg gtggttcgcc
    18541 acatcacccc gcctccgcca gttttgctgg gcattctggt tgctgatgtt agttacgctc
    18601 atttttctat catcgacaca ccatgaagag cgcgacgcat taattcgact acgggcaagt
    18661 catcaccagc agtgggccgc actgtatcgc ctggtagaca ccactccctt cagcgaggaa
    18721 aaaacgctgc ccttttcgcc actggatttt cagttatccg gcgcgcaact ggtttcctgg
    18781 catccatccg cgcagggagg cgagttggcg ttgaaaacgc tgtgggaagc agtgccgtcg
    18841 gcatttacac ggctggcaga gcgcaacgtc agcgtgagcc gtttttcgtt aagcgtggaa
    18901 ggtgatgatc ttttgttcac gctacaactg gagacgccgc atgagggtta aacgctggtt
    18961 gttggcaggt attgcattgt gccttttaac cggtatgcgt gaccctttta aaccgccgga
    19021 agatctatgc cggattagcg aacttagcca gtggcgctat caggggatgg tagggcgagg
    19081 cgagcgcatc atcggtgtaa taaaagacgg gcaaaagaaa tggcgacggg tgcagcaaaa
    19141 ctatgtgctg gaaaacggct ggacaatttt acagctgacg ccagacgtac taacgctggg
    19201 taccgggaca aactgcgaac cgccacaatg gttgtggcaa cggcaaggag atacaaatga
    19261 agcaatggat agccgcacta ctgttgatgc tgatacccgg cgtacaggcg gcaaagccgc
    19321 aaaaagtgac gctgatggtg gatgacgttc cggtagctca ggtgttgcag gcgctggctg
    19381 aacaggagaa gttgaacctg gtcgtgtcgc cagacgtcag cggtacggtg tcgttacatc
    19441 taacagatgt tccctggaag caggcactac aaactgtagt gaaaagcgcc ggactgataa
    19501 cgcggcagga aggcaacatt ctctcagtgc attccattgc ctggcagaat gacaatatcg
    19561 cccgccaaga ggcggagcag gcgcgggcgc aggcaaatct gccgctggaa aatcgcagta
    19621 taaccctgca atacgccgac gcgggagaac tggcgaaagc gggggagaag ctactgagtg
    19681 ccaaagggag tatgaccgtc gataaacgca ccaatcgcct tttgctacga gataacaaaa
    19741 cggcgttaag cgcgcttgaa cagtgggtag cgcaaatgga tctgccggtc gggcaggttg
    19801 agctgtcggc gcatattgtc accattaatg aaaaaagttt gcgtgattta ggcgtgaaat
    19861 ggacgctggc cgatgcgcaa cacgctggtg gcgttgggca agtcaccacg cttggtagcg
    19921 acctctccgt agcgacggcg acaacgcatg tcggttttaa cattgggcgc atcaacggac
    19981 gcttgctgga tcttgagctt tccgcgctcg aacaaaaaca gcagctggat attatcgcca
    20041 gtccgcgtct gctggcctca catcttcagc ctgccagcat taaacagggg agcgaaattc
    20101 catatcaggt ttccagcggg gaaagtggcg cgacgtcggt ggaatttaaa gaggccgtcc
    20161 tggggatgga ggtcacgccc acggtgttac aaaaaggtcg catccggctg aaattacaca
    20221 tcagccagaa cgttccgggg caggtgctac agcaggccga tggcgaagtg ctggcgattg
    20281 ataagcagga gatcgaaacg caggtcgagg taaaaagcgg agaaacgttg gcgctgggcg
    20341 gcatttttac ccgtaaaaat aaatcgggtc agga
//
LOCUS       DADLSB010000067        19971 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.067, whole genome shotgun sequence.
ACCESSION   DADLSB010000067 DADLSB010000000
VERSION     DADLSB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.067"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..758
                     /locus_tag="JN331_002289"
     CDS             <1..758
                     /locus_tag="JN331_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAY7260421.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSEAQHYDFIKKKSIYEKLNSKSSFD
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(827..1783)
                     /gene="yjiA"
                     /locus_tag="JN331_002290"
     CDS             complement(827..1783)
                     /gene="yjiA"
                     /locus_tag="JN331_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY7260422.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQMGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1794..1997)
                     /locus_tag="JN331_002291"
     CDS             complement(1794..1997)
                     /locus_tag="JN331_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7260423.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2047..4197)
                     /gene="btsT"
                     /locus_tag="JN331_002292"
     CDS             complement(2047..4197)
                     /gene="btsT"
                     /locus_tag="JN331_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY7260424.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4590..5102)
                     /locus_tag="JN331_002293"
     CDS             complement(4590..5102)
                     /locus_tag="JN331_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY7260425.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5120..6682)
                     /gene="hpaB"
                     /locus_tag="JN331_002294"
     CDS             complement(5120..6682)
                     /gene="hpaB"
                     /locus_tag="JN331_002294"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HAY7260426.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            complement(6909..7765)
                     /locus_tag="JN331_002295"
                     /pseudo
     CDS             complement(6909..7765)
                     /locus_tag="JN331_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7873..8763)
                     /gene="hpaA"
                     /locus_tag="JN331_002296"
     CDS             complement(7873..8763)
                     /gene="hpaA"
                     /locus_tag="JN331_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY7260427.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8773..10128)
                     /gene="hpaX"
                     /locus_tag="JN331_002297"
     CDS             complement(8773..10128)
                     /gene="hpaX"
                     /locus_tag="JN331_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY7260428.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMAIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            complement(10150..10893)
                     /locus_tag="JN331_002298"
     CDS             complement(10150..10893)
                     /locus_tag="JN331_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY7260429.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGLADLSADMGYAGNPQHPEVQAANEQLAKRYLELGALFVAVGVDTTL
                     LARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(10904..11707)
                     /gene="hpaH"
                     /locus_tag="JN331_002299"
     CDS             complement(10904..11707)
                     /gene="hpaH"
                     /locus_tag="JN331_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY7260430.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11775..12155)
                     /locus_tag="JN331_002300"
     CDS             complement(11775..12155)
                     /locus_tag="JN331_002300"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY7260431.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMEGRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(12165..13016)
                     /gene="hpaD"
                     /locus_tag="JN331_002301"
     CDS             complement(12165..13016)
                     /gene="hpaD"
                     /locus_tag="JN331_002301"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY7260432.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(13018..14484)
                     /gene="hpaE"
                     /locus_tag="JN331_002302"
     CDS             complement(13018..14484)
                     /gene="hpaE"
                     /locus_tag="JN331_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY7260433.1"
                     /translation="MKKVNHWINGKNVADNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTTTGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(14481..15770)
                     /locus_tag="JN331_002303"
     CDS             complement(14481..15770)
                     /locus_tag="JN331_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY7260434.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            16042..16488
                     /gene="hpaR"
                     /locus_tag="JN331_002304"
     CDS             16042..16488
                     /gene="hpaR"
                     /locus_tag="JN331_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY7260435.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAEST
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            16607..18271
                     /gene="tsr"
                     /locus_tag="JN331_002305"
     CDS             16607..18271
                     /gene="tsr"
                     /locus_tag="JN331_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7260436.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPCQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(18320..19681)
                     /locus_tag="JN331_002306"
     CDS             complement(18320..19681)
                     /locus_tag="JN331_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7260437.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSIRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREKLGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
BASE COUNT         4846 a         5088 c         5247 g         4790 t
ORIGIN      
        1 attatactaa gattttagag aatgattttg aacacccttc aatacgacga aaaatagcat
       61 cgctaagtaa tttaggggta gatgagtgga ttcctccggt tatggcattc atgaatcgaa
      121 tggctcgtac agaagatttt aatttagatg atttttctca atttattacc gcctttgaga
      181 aggtatatat gcatgggtgg ttaaaaaaac aaattaaaag ccaaagagaa atggtttgtt
      241 attcagctct ggtagcaata aataatgata tgccttttga ttctgtgata aatcagataa
      301 accaacatgc agataattca ggttttatag ctgctctgga tgaagattta tatgagcctc
      361 gtccgaatca agtgaactta ataaaagcaa ttttgcttcg cttagatatg gagcaacaag
      421 atgagagcgt gattaaaacc tatacgggga gaatcacaat tgagcatatt cttcctcaag
      481 cattagttaa tgaatactgg attaatagat tccaacctca agagcatgtc tactggctgc
      541 ataaaatagg taatttaaca ctcataagtg gttccaaaaa ttctgaagca caacactatg
      601 attttatcaa aaagaaatct atttatgaga aacttaatag taaaagctct tttgatttaa
      661 caaaagatgt ttgtaattct tcagaatggg gattggctga actgaaaatg agacatgaaa
      721 aaatgaaaac gcaattgaag aagctttggt tggtctgaca tatctaaaga gttccactcg
      781 taataatgtt gcggtggaac tctcaaaaag ttgatctttc ccgaacttac ttcctcaatc
      841 ccgcaaacgc cgcccgaatc tcttcctccg gcaactgaat cccgataaac accattgtgc
      901 tatgcggttt ttcatcgccc cacggcctgt cccagtcggc gctgtagagg cgctggacgc
      961 cctggaacag caggcggtta ggttcgccgt caatccacag catccctttg taacgcagca
     1021 gtttatccgc cgattccagc agcaggtttt ccatcacgcg ggaaacttcg ctgatatcta
     1081 ccgggtaatc cagttccacc acaattgacg aaatatcgtt ttgtttatcc gcgataaagt
     1141 ggaaacgcgg tttggtgctg acgacgttct cttccagcat aaaaccgttg gtgttgaaca
     1201 gcaggcccag gtcgatgtcg ccgtgggtga cggtgtagac cggtgcgcgg gcgttgatgc
     1261 gcgccaggcg ttcacgcagt ttctctgctt cgcctgcgac gtcggtttta gtcagcagaa
     1321 tacggtcggc gtagcccatc tgggactggg cgatggtgaa ctggttcatc tgctcatcgg
     1381 catgtaccgc atccaccagc gcaatcacgc cgtccagcag gtagcgctgg cataaaatct
     1441 catgggagaa aaaggtctga ataatcgggc cgggatcggc catgccggtg cattcaatca
     1501 ccagacgatc gaactgaata ttgcccttgt cgagattatc cagcaggtcg agtagtgcgt
     1561 cctccagctc gttggagcgc gaacagcaga tgcagccgtt ggtcagcgtt ttgatctgcg
     1621 tggcgcggtc gccaatcagt tgatcatcaa cggagacttc gccgaattcg ttttcaatca
     1681 cggcaatctt gtagccgtgt tgttcgttaa gaatatggcg cagcagggta gttttgcccg
     1741 cgcctaaaaa accggtgagt agggtaactg caatcgggtt catgctctct ccattaacaa
     1801 cagcgcatac cgcctttacc atcgccgccg tagcgcgcgt tctggcgttc gcggaagaat
     1861 tcttcatagc tcatgtacgg cttgtcggga tggttggtct tcatatgctc gacgtagttg
     1921 tcgtagtctg gaataccaat caacatcttc gccgcctggc cgagatattt ttttgcctgt
     1981 cctaagttac caaacatagt tcactctgat aagaacaaag ccccgccgaa gcggggctaa
     2041 acacggttag tggtgcgaag agatcttcac gccgccttcc gggattggaa cgtacggtgt
     2101 ttctttgtca gtacgtttgt cgctgttacg caccgcaagc caggttttga aaccgtagaa
     2161 gatgatgctg tacaccacaa tcaggaacag aatactcagg cctgcgttgg tgtagttgtt
     2221 cacaacgatg tggttcatgt tggcaatctg ctgcgccgtc aggtcagtac cgttagcaat
     2281 cttctctttg tactggcttg ccatgtagaa gaagccttcc atctgcgggt tggtgctgaa
     2341 cagtttcaga cccagcgccc aggtggtgca gataagcagc catacagccg gaacaacagt
     2401 tacccagatg tattgggtgc gcttcatctt aatcagcaca acggtgccca gtaccagcgc
     2461 tacggctgcc agcatctggt tggagatacc gaacagcggc cacaggctct taacgccgcc
     2521 cagcggatcg accacgccct gatacagcag gtagccccac agacccacac agcccgcagt
     2581 accgatgata ccggcaacca gagagtcggt ttttttcagg aacgggatga agttacccag
     2641 caggtcttgc agcataaagc ggccagaacg ggtacccgca tccagcgcgg tcaggatgaa
     2701 cagggcttcg aacagaatac cgaagtgata ccagaagccc atgtcagcca tcggcagcac
     2761 tttgtggaac acgtgagcga tacctaccgc cagcgtcggc gcaccacctg cacggttcag
     2821 gacagaaggc tcaccaatgt ctttcgcggt ttgcaggatc tgctctggcg aaatcacgaa
     2881 gccccaggag ctgacggtcg ctgccgcgtg tgcggtaacg tctttcagct gcgccatgat
     2941 gatcggcgcg ttctcgccac ccatttcatg caggttaggc atggtgatgc caaggccagc
     3001 aggcggggtg ttcatcgcga agtaaagacc cggttcgatg atggacgctg caaccagcgc
     3061 cataatcgcc acgaaggact ccatcagcat tgcgccgtag ccaatgaaac gcgcgtcggt
     3121 ttcgttagcc agcagtttcg gcgtcgtacc ggaagagatc agcgcgtgga agccagatac
     3181 cgcaccacag gcgatggtga tgaacaggaa cgggaacaga gcgcctttcc acagcgggcc
     3241 agtaccgtca atgtactggg tcatcgcagg cattttcagt tccgggttca gcaccacgat
     3301 acccagcgcc aggccgacga taacgccgat tttcaggaag gttgccagat agtcgcgcgg
     3361 tgcgaggatc agccacaccg gcagcagtgc ggaaacaaac gcatagccaa tcagcgcgaa
     3421 ggtaatggtg gtgtctttaa aggtcagtgc cggaccccag tacggatcgt gagcaatcac
     3481 gccaccgaag tagatagagg caaccagcag cacgatacca ataacagaga cttcacccac
     3541 acgccccgga cggataaagc gcatgtagat acccataaac agcgcaatcg gtacggttga
     3601 gcaaacggtg aagacacccc acggactttc ggccagggct ttaaccacaa tcagcgccag
     3661 aacggcgagg atgatgatca tgattaagaa acagccaaac agcgcgatag tccccggtac
     3721 tggccccatc tcttctttga tcatctcacc gagagatgcg ccgttacggc gagaggagat
     3781 aaacagcacc ataaagtcct gtaccgcacc ggccagcact acccccgcca gcagccacag
     3841 cgtgccaggc aggtagccca tctgcgcggc gagaaccggt cccaccagcg gaccagcacc
     3901 ggcgatagcg gcgaagtggt gaccaaacaa cacgtaacgg ttggtcggaa cgtagttcag
     3961 accgtcgttg ttaataaccg caggcgtcgc gcgcgtgggg tcgagtttca tcaccttctg
     4021 ggcgatgtac agactgtagt agcgatacgc caccagatac accgacacag aggcgaccac
     4081 gatccacagg gcgctgacgt gctccccccg acgtaatgca actaccgcga ggcagaatgc
     4141 accgatgatt ccgagaatca cccagggtat gtgcttgaat agttttttcg tatccatagt
     4201 aaaacctggc attttactcg tcgtcttttg gcttacaggt gcggcagctg cgttcgttca
     4261 cctctgacac ttacttttgt aagctcccgg ggattcactc acttgctgcc ttcctgcaaa
     4321 ccaaaatcca tagagtaatt tgttaatcat aggccgaagc cgttctaggt ttgcgttgcg
     4381 tttgaggagg taaattgacc gctatcctgc agtgatgttg ccagagatac gtgcgcgtaa
     4441 agtaaggtaa ataactgagt ggttatttta gggatgtaag cggtcaggaa tggcggtgag
     4501 cggttgggca agaattattc atcgggtaga tatgtgattg agatcacaat aaaaatgccc
     4561 cttcgacgaa tgcgtgaagg ggctggagct taaatcgcag cttccatttc cagcatcacc
     4621 ggatggaaac ggcgtttaaa gtaaataagt ccgtgacctt ctgcactgag gatgatgttt
     4681 ttaatctcca ccagatacac cagatgtgtg ccaatcgcct gcacatcgcg gatctctcct
     4741 tcaagactgg ccagcgaacc ttttagcacc ggctgcgcca gcggaccttt ttgccagcat
     4801 gagaggctaa aacgctcttc catcgccatg cctgtcatgc ccgcgaagtg gcgcgccatc
     4861 agttcctgct catggttgag gacgttgacg cacaacttgc cgttgccctg aaaaaccggg
     4921 ttcatcgcac tgttggcgtt aatgcacacc atcagcgacg gtggtgtatc cgtgaccgag
     4981 cagacagccg ttgccgtaat cccgcattgt ccggcgtcgc cctcggtggt gataatattt
     5041 accgctgccg acaggctggc catcgcgtca tgaaagcgca ggcgttgttc atctaattgc
     5101 atcttaacct cctgctgcgt tatttcagca gcttatccag catgttgata tcgtcgttgt
     5161 tgtgcaggtg cggcacagtc cagccgttct ggtcgtattc cgacaggcag cgatcaacca
     5221 tcgccatcat cttgtccata ttgccggagc tttgtgcctg gcgcagacat tgcaggcgaa
     5281 tctcatcctg gctaccggag tagttgattt catacagctc gtgacgacca ccaaattcgc
     5341 tgccaatggc gtcccacatc agtttgagga tcttgatgcg ctggacgtga tccataccgt
     5401 tcgaaccgcg cacatacttc gccagatact ggtcgatctg cggattgttc aggtcacggg
     5461 cactggaagg gaggtagatc aggccactgg taacgttgcg ttctataatg tttttgatct
     5521 tcgcgtaggc cattggtgcc agtacgcgat aggtttgcag tgcggcatga tccggtaaat
     5581 aagccccgtt gacccacggc gtcgcttctg aacacatcga gtcactcaat gcccagaagg
     5641 tgttgcgcca cgccaccact tcaccgagat cggcctgcac accacggaac tccagggtgc
     5701 cggtacattc gagtgatttt ttcagcagtg ccgtaatgaa gtcgagtttc actgccaggc
     5761 gcacacaggc ttgcagcgga tacatacggg cgaaaccgcc ttccatcgtc cagcgacggc
     5821 agcgatcaaa atcgcggtag atcagcacgt tttcccatgg gatcagtacg ttatccatca
     5881 ccagaatcgc atcgttctca tcgaagcggc tggagagcgg gtagtcgtat ggcgagccgg
     5941 tagcacccgc gaccatctca taagaggcgc gggagattaa tttcacgcca tcggcatcca
     6001 ttggcgcaac gaacatcagt gcgaagtccg ggttttcgcc catcacttgt gccgagccga
     6061 agccaatcat gttgtagtga gtcagcgccg agttggtggc aaccactttc gcaccgctga
     6121 cgataatccc ggcgtcagtc tctttttcca gcttgatgta aacgtctttc actttatcgg
     6181 tcggcaaatg acgatcgatc ggtgggttaa caatcgcgtg gttaaagtag aggccagttt
     6241 cctgaatacg ggtataccag ttacgggcgt tctgctcgaa ctgaccgtaa aagcccggat
     6301 tcgcgcccag tgcgcaaccg aaagcggctt tgtagtctgg ggtacggccc atccagccat
     6361 agctcaggcg tgaccattca gcgatggcgt cgcgttgctg gcgcaggtcg tcggcacttt
     6421 tcgccacgcg gaagaattta tgggtatagc cgccgctgcc ggtgtcggtg ttccaacaca
     6481 gagagtcctg catctccggt ttgtgcagcg cgtcgtacag ttgggcaaca gacgctgccg
     6541 cattacgaaa tgccggatga gtggtgacgt ctttcactcg ctcgccatag atatagatct
     6601 cgcgaccatc ctgcaggctt ttcagatact cttccccggt gaaaggacgt tgggtactgg
     6661 cgcggaaatc ttctggtttc atgtcgacct ctacagtatt aatcggaata cgattgttaa
     6721 ttttgtgtgt tgtttttgtt atttaattga agcgttgaac gcggttgtcg ggaagagacg
     6781 tttcaggcga acgttggtac ttttcgacag caaggtggag ttaaatagta aactgcgatc
     6841 gtgatcaact tacagtaagg caaatcataa tattccccgc taacaagggc taacggggac
     6901 ggaatttttt atggggcgaa tggaatggta atcgtcatat tttcatgacc aacgacgata
     6961 gggcctgaat agttttctgt gcttcttgct tccatgtttc atccggaacg gtgattggta
     7021 ccaggtggct aaggatgagt tttttcactt ttgcttcatt ggcaattttg cctacatcct
     7081 caatagtcgt atggtgagaa gcaatggctt ttgccagtgt tttaccattg ccaatactgt
     7141 ctgccagctt agcgacggag ggctcatgta cggcctcatg cacaagtata tcagcgcctt
     7201 ctgagaaagt tgccagtggg ggaaagtacg aggtatctcc tgagaagaca attcgcttgc
     7261 cttgtgtgtc gaagcgataa gcaaaagcct caccgtcagg gaaaggagaa tgagggacct
     7321 ttaatgcagt cacggtcacg gtatcatctt tataaactac gccttcacca acatcttgag
     7381 ctgtaacgag tttgcgagga tcgggttttc cttcttcttc catacgcagt gtgatgtccc
     7441 gatcgaagac ctgccaactg gcagcggtca tggctcgggt gcctggcgga ccgaatacat
     7501 gaatagtttt gtcctgcaga ccgctagccc atgcattcat taagactgat ggatagtcca
     7561 gaatatgatc actgtgtaaa tgggtgagta aaatattatc aatctggcgc agtggaatat
     7621 tattttttac cagttgtaag ctggccccgt aaccagcatc aattaactag gttttattgc
     7681 caataataag tgccgttgcc atcggtaaac ggtcagcttt taataaagaa gggccacctt
     7741 tcgttccaag tagtacaatt ttcatttatt gcggttcgga ggcgtaggtg aatgccactg
     7801 gcgcaatcag gcacgccaca aatagtgagc gtaaagatat tttcatgtcg agtctctcag
     7861 gttgataaat ggtcagcgac cacggaattg tgttggtgag caacccatca ttcggttgaa
     7921 aaaacgggcg aaataggcgg gatctttgta gccaagatca agggctatct ggttgatgct
     7981 ttgctcgcta tagagcagaa gccgtctggc ttcccggctc actcgttcga aaatcaatct
     8041 cttcggcgat tgattggcaa aacgtcggca cagctcggta agccgtgatt cactcatacc
     8101 gagcattgtt gcatattcag ggaccattaa atgttggcga taattttcgt caatcagccg
     8161 attaaacttc tggaataaac acatctcgcc gcgcacgctg catgttgaaa tatcatttgg
     8221 tggtgcttca cgcagtagga ttgtaaaaat actttgcgcc agtgattgca atagaatttc
     8281 ccgtcctggc ttattttgtt gaaactccag ggatattatc ggccaataat gatttaatgc
     8341 cgccagtgta tgttccattc cctccagtga cagacagata ccttgtagca ttgcggcatc
     8401 tccactacca ggccagagct tttctaccag gggccaaatt aactcctgac gtacggtcag
     8461 aacatgccca tcgctatcag gatcagtaaa gaaagtatgt ggcaccgatg gtggtgttag
     8521 tacaaaaagc ggtgcgcgca cagaatagaa gtgctcatcc agttgtaaag taatgcgtcc
     8581 agtgaccaga taatggagtt gaaaatatct atcgtgccag tgagggcgca tatcgcgtcc
     8641 aaagaaattc gctaaccggg caaatgtttc ataatgcaca tcttcaccag cgtagcgggc
     8701 atcatattcc cggaccaaat caatattttc ataaccatgt tcaaagatag cttcactgga
     8761 catcatgact ccttattgcg gttttggtgc gataagttca gatgaaagag agtgttcccg
     8821 actccccata gggattcggg tcatgactaa cgcgcccagt accagcaatc ctgctacaaa
     8881 ccacaaacct gagctaaaac tgccggttgc atctttcaaa ataccgataa gtaatggact
     8941 gactgctgaa cctacattgc cgacagcatt gatgacagcc atcgcaacgg cacgcgattg
     9001 caagcttata acttgatccg gtgttgtcca aaagatagcc atcgcggtaa atgatcccat
     9061 tgaggccata atgatgccgc tcagttgaat gaggctgtga tctgtcgcag aagcgagtaa
     9121 ccatccggca gcggcaaaca ggtagggaag aatcgtatgc tttttgcgtt ctttaagacg
     9181 gtcggaacga cgactccacc agatcatgcc aaaaattgtg cagaattggg ggatggccgc
     9241 cagcaaacca atcatgatgt tgctactgcc agtattgaaa ctctgtagga tttgcggcgt
     9301 ccagatatta atcgcactca gagtattggt caggcaaaaa taagcaagtg tatatagcat
     9361 gatggctggg gtcaggactt cacgtaaggt tgagcgtgga acagatgcat gggcaaatgc
     9421 actctcttgt tcacggagaa tcatcgtttt taaagcctgt ttttcgtttt catcaagcca
     9481 ggtcgcctgg tctggtgtgt cgttgagata aaaccaggtg acgacaccaa gaatgaccga
     9541 tggcagacct tccagcaaaa agagccattg ccagcctttt agattccaca gtccatccaa
     9601 ggccagaatg tagcctgaaa ggatcaatcc cagcatcata gtgactggca tcgctatcat
     9661 aaataaggca ttggctctgg cccgatgata tgccgggaac caccaggtca gataaaccaa
     9721 aataccaggt aaaaaacccg cctcagcaat accaaccagc atacgcagta gatagagtgt
     9781 ttcggggctt gttgcaaaca ttgtgcaggt ggaagcaatt ccccagacca ccatgatccc
     9841 agcgatccat cgacgagcgc caattttcgc taacatcata ttactgggga tcccgcacat
     9901 gacgtacgtg acataaaaga gtgtcgcggc gaggccaaac attgtggacg tcaggccaag
     9961 atccttgccc attgtcagtc cggcaaatcc tatattgatg cgatcaagaa acgaaaaaac
    10021 aaacagaata aaaagaaaaa tgattaggcg acgaaataat ttattaatga cacgatgttc
    10081 aatttcaaca gttttattat ctttatattg cagggtagag gtcgtcatga tgcgctccac
    10141 atcgtacgtt tagtagacag agttgttgtt ttgcttcgcc atcgaaatag aagaactatt
    10201 accaaaccgt gctgccagcg cttcggcggc gcgggcgagc agggtggtgt caacgccgac
    10261 ggcgacaaac agcgcgccca gttccagata gcgttttgcc agttgctcat tggcggcctg
    10321 tacttccggg tgctgcggat taccggcata acccatatcg gcgctcagat ccgccaggcc
    10381 gataaacacg ccgtcgaccc cttccacgtc cagaatctgc ggtaagttct tcattgcctc
    10441 acgcgtttcg atctgcacca gcacgcacat ttgatcgttg gctttttgca ggtaatcagg
    10501 aatgcgattc cagcgcgagg cgcgagccag cgcactgccc acaccgcgaa taccggcggg
    10561 gggataacgg gtggcgcgta ccgctttacg ggcttcgtcg gcgttttgta ccatcggcac
    10621 cagtaaggtt tgtgtgccta cgtccagcag ttgtttgatt tgcaccggat cgttccacga
    10681 tgggcgtacc actggctggc tggggtaggg cgcaatcgcc tgtagttggg tgagcacggt
    10741 ttgcacgtta ttcggcgcgt gctcaccgtc gatcaataac cagtcgaatc ctgctccggc
    10801 cagtaactcc gcgctgtagc tgctgctcag ccccagccat aatccaatct gcggacggcc
    10861 tgctttcagc gccgctttaa aactgttttc catcgttctc tccttaaaca aagcggcagc
    10921 taatggagcc catgttgccg taatcgacgt ggaaggtgtc gcccttacgc gccggaaccg
    10981 ggcgggtgaa cgaaccaccg agaatgattt gcccggcttc cagttgtacg tcatacggtg
    11041 cgagtttgtt cgccagccag gccacgccgt ttgccggatg attcagcaca ccagcggcga
    11101 cgccggtttc ttcaatcacg ccattgcgat acatcagggc ggagatccaa cgcagatcca
    11161 gttcgtctgg tttgatcgga cgacctccga ggatcactcc ggcgttggcg gcgttatcgg
    11221 aaatggtgtc gaatactttg cgcgggcgct gggtttccgg atcgatgttg tggcagcgag
    11281 cgtcgatcag ctccagcgcc gggatcacat agtccgtggc gttgtaaacg tcgaacagcg
    11341 tgcagtttgg cccacgcagc ggttttgcca gcacaaaagc cagctccact tcaatgcgcg
    11401 gcacgataaa gcgatcggtc gggatatcgc tgccatcgtg gaagaacatg tcgtccagca
    11461 gtgcaccgta atccggttcg ctgatctgcg agcttgcctg catcgctttc gaggtcagac
    11521 cgattttgtg gcctttcagc gtgcgacctt cggcgatttt caggcgaacc cattcgcgct
    11581 gcacggcgta ggcgtcttcg atggtgatct ccgggtaatc cagcgagatc gcgcggatct
    11641 gttcgcgctg tttttctgcc tgatccagac gctgggcgat cagggtgtgg gtgtgtttat
    11701 cgaacatagc gatatcctgt ggcattgccg gatgcggcag cgccttatca tccggcctgg
    11761 aagctggtgc aaatttattt aaacaacgcg tgtacgttgt tctgtttgaa attcaacgtc
    11821 gggtgcagct ctgcaatctc aaatgaaagc gctaggtaac ggccctccat cagtgaggcg
    11881 aagtgcgttt taatcagatc aaacagcatt tcgcccactt cctgacggct ttccagactg
    11941 cgtccgctgc cgatttttag cgtcatatgc acaaaggcgt aatcgtgctt gccatcagcc
    12001 atttgccagg tatcgatcca gtgcgcccgg ctacgtattc ccccaagcgg aaaaatcccc
    12061 gttgccgcca gtgcagtgtt aacactggca aacagctcag gtaagcgggc ttcttcacga
    12121 atattttcag tacattccac gataaagtgc ggcataaaac gtccttacgc gggcagcggg
    12181 aaaacagcgt taacctgacc ggtgccagag cttgggaaca gctcggtaat aaactccacc
    12241 ttgccgtcgt atttatccca gccgagcatc cccagcagca tcaccgtgtc gtgcatattg
    12301 ccttcgccgt agcagtagtc ggcgtactcc ggcagcatat tgcaaaactc tttgaactgg
    12361 ccttcgcgcc acagcttcac cacacgctcg tccatctggc ggtcgaactc gcgggtgtag
    12421 ctgttcatcc cttcttccgc acgctgatcg tcaataaagc ggtgcgataa cgaaccgctg
    12481 gcaaggaccg ccacggtgcc gtcgtactgt tcgatcgctt tcagaatcgc ttcgcccagc
    12541 ttgcggctgt cggcaaaatc gtgaaccgtg cagaaagccg aaatggagac cactttgaag
    12601 tgcttgtctt cattcatgta gcgcatcggt accagcgtgc cgtattccag tttcaggctg
    12661 ggaatattgt gcgcttttgc ccgcacgccg agctttaagg cttcctcggc aattagctgc
    12721 cccaactccg ggttgccctc gtagttgtag gtcatgtcgc gaataaaatg cggcagctcg
    12781 ttgctggtgt agacgccttc aaaatggtct gcacagttga tgtgataagc actgttgacc
    12841 agccagtgag tatcgaaaac gataatggta tcgacgccca tttcccggca acgcttgctg
    12901 atctctttat gcccgtcgat cgcaccctgg cgacaaccgt ggttttttcc tggtagttca
    12961 gagagataca tcgacggtac gtgcgtgatc ttggcggcta acgctaactt acccatatca
    13021 gactccccat ttcggaattg gatggtcgcc catggaaatg cagacgttct tcatttccgc
    13081 gaacacttcg aagctgtact caccgccttc acgcccggtg ccggaggctt ttacgccgcc
    13141 aaatggctgg cgcaggtcgc gcacgttctg ggtgttgacg aacaccatgc ctgcttcaat
    13201 gccacgcgcc aggcgtaaca ctttgctgac atcctgtgtc cagatgtacg acgcgaggcc
    13261 gtactccacg tcgtttgcca ggcgtaagcc ttcggcttcg tctttaaacg gcagcaggca
    13321 ggcgaccggc ccgaaaatct cttcctgggc aacgcgcata cggttatcta cgtccgccag
    13381 caccgttggg cgcaggaagt tgccgccttt caggtgcgca ggcaggtcgg acggtttatc
    13441 cgggccgccc gccaacaggg ttgcgccttc ttcaatgccg agacggatat agccggagac
    13501 tttttcccag tgttgctgac tgataagcgc cctaacctgg gtattcggat cgttcggatc
    13561 gcccacgcgc agacggttgg cgcgttcggc aaagcgttta acgaattctg gatagatgct
    13621 ctgttgaata aagatgcgcg aaccggcggt gcagcgttcg ccgttgattg agaagatggt
    13681 gaacagggcg gcgtccagcg cgcgctcaat atcggcatct tcaaaaatca gcaccggtga
    13741 tttaccgccc agctccatgg agtatttttt cagcccggcg tttttcatga tgttgcgccc
    13801 ggtggtggta ccgccggtga acgacacggc acgcacgtca tgatgacgga ccagcgcatc
    13861 gcctgcggtt gcgccgtagc cctgtaccac gtttagcacg cccgccggaa taccagcttc
    13921 cagcgccagc tcacccaggc ggtcggcggt cagcggggag agttccgaca tcttcagcac
    13981 cgcggtattg cccagcgcca gacacggcgc gaccttccag gtggcggtca taaacggcac
    14041 gttccacggc gacaccagcg cacaaacgcc taccggttgc accagcgtgt agttgagcat
    14101 cttgtcgtca accggatagg tcttgccgtt catctgctgg cagacttccg cgaaaaattc
    14161 aaagttgtga gaagcgcgtg ggatcaacac atttttggtc tgatggatcg gcaggcccgt
    14221 gtccgcggtt tccatcgcgg cgatctcagg cacgttctgg tcgatcagat cgcccagacg
    14281 gcgcatcagg cgcgcacgct ctttcatcgg cagatcagcc catttcggga acgcctcttt
    14341 cgctgccgct accgcctgat tgatctccgc ttcaccgcca gaggccacat ccgccagcac
    14401 ttcacccgtt gccggattgg tagtctggaa gtagtcgtta tctgcaacat ttttgccgtt
    14461 gatccaatga tttacttttt tcatttcgct gtttcctcac tcacaattcg gttcaccagg
    14521 cggcccacgc cttctacttc caccactact tcatcgccag gcaccacgtc agataagcct
    14581 tttggtgtgc cggtggcgat catgtcgccc ggatttaggg tcataaattc gcttaagtag
    14641 gcgatcagga agggcacgct gaagatcaga tcggcggtgg tgccttgctg gcgtaactcg
    14701 ccgttgacga aggtgcgaag ggtcagatta tgcgggtccg ggatcgcctc tttcggcacg
    14761 atggttgaaa gcatcggcgt cagtccgtcg cggcttttga cccgcaggtt agggcggtag
    14821 tagttttcca gatagtcgcg aatggcgtag tcgttacaca cggtgtagcc cgcgacataa
    14881 tccatggcat cggcttcgct gacgttacgc gcctgcttgc caataactac caccagctcc
    14941 gcttcgtagt gcatgtattc aatattgttc gggcgcacgg aggtctggtt gtcgccagtg
    15001 agggtattcg gcgctttcag gaacaccagc ggctcttccg gtggcttaaa ttccagttcg
    15061 ctggcgtggt cggcgtagtt caggccgagg gcaaacagcg taccgtgcgg gtgtggcagc
    15121 gttgggaagc tcttgcgcgt ggtcacttca cgttcgtcca ctaccggatt ttccagcggc
    15181 gggaaacctt ctgcgagaac acgcacgcga tcgcctggct gtatttccac gcgcgcctgt
    15241 ggcgtgccga gcagaatagc atcgcctgga ttcagcgtgg caaattcgct cagggcgctc
    15301 agcagctgtg cggcgttacg ttgtaaatcg gcggtattcc agtgatcggc aggacgcccg
    15361 ttgatctcgg tatagatagt cagattatcg acattgctga gagccacggt ttcgccaatg
    15421 gggcagaatc catcacgaca ttttgctttg attgccggac ggtaaaagct ctcttccggc
    15481 aggctgacgt cgttagccag cgcatatccg gcgatgtact ctgccgcatc ttcttcacgt
    15541 actttcgtcg ccgttttccc cacaatcaga gcaacagtcg caccgctcag tactttttca
    15601 ccctgtggaa agggaatcgg ttcaccgcaa ccaatcaccg tattgcgcgg tttaataaac
    15661 cagaccgcag ttttaggcgg ggctttgtag ggagattgct ggaacgcttc ctgccaggca
    15721 tcaagttggc tgcgatggtt caacgctacg gcgaagatag tgcctttcat taccactcct
    15781 cggattcgat catcttaatc attaatatgt taatgattac ttttattccc gattgcgtag
    15841 tttggcaaaa gtaagtgata taaatgtgat ctaattaaca aactatttac atttaaagat
    15901 aataaaatat taaatatcag taagttaata aaatatcatc aattttgata aaatgaagaa
    15961 ttgcgggggc agctcagtcc cggtaacgtg atgattaatt gtttctatat taatgatttt
    16021 gaatttttca ggggaaagat aatgcacgac tcactaacca ttgcgttgct ccaggcgcga
    16081 gaagcggcga tgagttattt tcgcccaatc gttaaacggc ataatttgac tgagcaacag
    16141 tggcgcatcg tgcgtattct ggcggaaagc acatcaatgg attttcacga tctggcgtat
    16201 cgcgcctgca ttttgcgccc aagcctgacc gggatcctca cgcggatgga acgcgacggt
    16261 ttagtgttgc gattaaagcc gatcaatgat cagcggaagc tctatatctc gctgaccaaa
    16321 gaggggcagg cgctgtataa ccgcgctcag acacagattg aagaagccta tcggcagatt
    16381 gaagctcagt ttactgccga gaaaatgcaa cagttaaccc atctgttaga ggaatttatt
    16441 gctctgggca attcccgaca agaagacatc ccgggcgata atgaataact ttttagtatc
    16501 tgataaccag cgcctggttt ttccatggat ggcgggttac accttttcat aaagtttttg
    16561 ctttccaggc cgaaaatctt gcatcggtcc acaggaaaga gaaaccatgt taaaacgtat
    16621 caagatcgtg accagcttac tgctggtttt ggccgttttt ggccttttac aactgacatc
    16681 aggcggtctg ttttttaatg ccttaaagaa tgacaaagaa aatttcactg ttttacaaac
    16741 cattcgccag cagcaatcca cgctgaatgg cagctgggtc gcgttgttgc agacgcgtaa
    16801 caccctcaac cgcgcgggta tccgctacat gatggatcag aataatattg gtagcggttc
    16861 aaccgttgct gagctgatgc agagtgccag tatttcgctg aaacaggcgg aaaaaaactg
    16921 ggcggattac gaagcgttgc cgcgtgaccc gtgtcagagc accgccgcag cggcagagat
    16981 caaacgtaat tacgatattt atcacaatgc gctggcggag ctgatccaac tgttaggtgc
    17041 aggcaaaatc aacgagttct ttgatcagcc gacccaggga tatcaggacg gtttcgagaa
    17101 gcagtatgtg gcttacatgg agcaaaacga tcggctctat gatatcgccg tcagcgataa
    17161 caatgcctcc tacagccagg cgatgtggat tctggtgggc gtgatgatcg tcgtactggc
    17221 ggtcatcttc gccgtctggt tcggtattaa agcctcgctg gtagcgccaa tgaatcgcct
    17281 gattgacagc attcgtcata ttgcaggcgg cgatctggtg aaaccgattg aggtggatgg
    17341 ctctaatgag atggggcaac tggcagagag tttgcgccat atgcagggag agctgatgcg
    17401 taccgtcggt gatgtgcgca acggggccaa tgccatctat agcggtgcca gcgaaatcgc
    17461 taccggcaat aatgatctct cttcgcgcac cgagcaacag gccgcttcgc tggaagagac
    17521 ggcagccagc atggagcaac tgaccgcaac ggtgaaacag aacgccgaga atgcgcgcca
    17581 ggccagccat ctggcattaa gtgcttctga aacggcgcaa cgcggcggca aagtggtaga
    17641 taacgtggtg cagactatgc gcgatatctc caccagttcg cagaaaatcg ccgatattat
    17701 cagcgtaatt gacggcatta ccttccagac caatattctg gctttgaacg cggcggttga
    17761 agcagcgcgt gcgggtgagc aaggacgtgg ttttgcggtg gttgcgggag aagtgcgtaa
    17821 tctggcccag cgtagcgccc aggcggctcg tgaaattaaa agcctgattg aagactcggt
    17881 ggggaaagtg gatgttggct ctacgctggt cgaaagcgcc ggggaaacaa tggcggagat
    17941 tgtcagtgct gtgacccgcg tgacggacat tatgggcgaa atagcttctg cttctgatga
    18001 gcagagccgt ggtatcgatc aggttggctt agcggttgct gagatggacc gggtaactca
    18061 acagaacgcc gcgctggtgg aagaatctgc cgctgccgcc gccgcgctgg aagagcaggc
    18121 cagtcgcctg accgaagctg tggcagtgtt ccggattcag caacagcagc aacagcagcg
    18181 tgaaacatcg gctgtggtaa aaaccgtgac gccagctacg ccgcgtaaaa tggcagtggc
    18241 agatagcggg gagaactggg aaacgtttta atcgccataa aaatgcccga taagcaaaat
    18301 gttatcgggc atagggagtt taatctttac gcgggtcgtt gatcggctgg cgaaccagga
    18361 agatgtacgc cagtgctccc gctgcggtga cgcaaccgca aatgattagt gccagacgga
    18421 atgagtgggt tgtatcaaca ataaaaccag taatgatcgg cgcgaaagag gcgcagatga
    18481 agctggcaaa gttctggata ctgcccaccg acgcagtcat gcgagaagca actgcgacgt
    18541 ggatcaagcc ccagcaggat gttccggcaa agtgaataca gaacagtgcc atgccaatca
    18601 gcagaaccgc catcatggat gttgtcgctt gtggtactat cagcgtaaag gcggcagaac
    18661 agaacatccc ggtaataatg cagatcttac ggcttttaat cggagccatt ccccctttga
    18721 ccagccagtc agtaacgtaa ccgttgacca gcatcccggc agccccaaac aggaaaggga
    18781 tagccgccat caaccctgtg ctttttaaat ctaggttata ggctgtttgc aggtaaccag
    18841 gaagccaggc cagatacagc cacgcagtgt agttgatgcc actgaatccg agcatcattc
    18901 cccacattgt gcggttacgg aacaggctgc gccattcggc aaaattgagc ggatctcggc
    18961 gggcattgac gctacctgca ttgagataag cttgttcaac ggcagtcagt tctacgtgct
    19021 cgcggttgcg atagagcata taccagccga tggcgagaaa aatccccagt acaccaatgg
    19081 taataaacat cccgcgccag cccatcacca gcatcattgc cgccagaatc ggtgggctta
    19141 cagcaacgcc aatggtagaa gctgcgttga agaagcccat cgggcgtccg cgctctttga
    19201 tgttgaacca gtcgttaatg acttttacac cgcatgggtt catcggcgct tcaccaatcc
    19261 ccataccgat acgcaccaac acgaactgcg taaagctgtg caccatgcca gacatggcct
    19321 ggaacagtga ccagaagaac atccccagtc ccagcatcag gcgtgggcct ttacgatcca
    19381 atagtgggcc gcaaggaagt tgcgcaatgc cgtaagcgag tgaaaacacg gagagcaaag
    19441 cgccgatttc ggtggcactt aatcccaatt tttcacgaat cgttaaattt gctaccgaca
    19501 gcgaactgcg gtcgagataa ttgattaccg ccgcaaaaaa taataacaac atggcggtgg
    19561 tttgaatgcg tttaattcgg atactgcgtt gaactaatcc atctggtggc acgggaatat
    19621 ctgccgacga agatggagta aatgaagaac gcggatcgag ggtgatattt tctttttcca
    19681 cgccggactc ctgtatttat aattttaatt gtttacgtaa ttgtgcatta cgcaaggttg
    19741 acattaacgg caggttaaag ttttaggact ttactaccgc tcacaaaaac atttttagtc
    19801 gactgattgt gagcataggt aagcgggaat tttccggtat ttatcttttg ataaaaccgt
    19861 gcacccgcta atagtttgct gtttttaatc cgtacttaat gagcataacg cgtgttctca
    19921 ttaatggatc gaatcattga ttgtttagct gaattcaaat ggttacggag c
//
LOCUS       DADLSB010000068        19838 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.068, whole genome shotgun sequence.
ACCESSION   DADLSB010000068 DADLSB010000000
VERSION     DADLSB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.068"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..441
                     /locus_tag="JN331_002307"
     CDS             <1..441
                     /locus_tag="JN331_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HAY7260438.1"
                     /translation="DHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNLTAADGL
                     AVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMAGPQRVC
                     ASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(549..2087)
                     /gene="emrY"
                     /locus_tag="JN331_002308"
     CDS             complement(549..2087)
                     /gene="emrY"
                     /locus_tag="JN331_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY7260439.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAICYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2087..3250)
                     /gene="emrK"
                     /locus_tag="JN331_002309"
     CDS             complement(2087..3250)
                     /gene="emrK"
                     /locus_tag="JN331_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY7260440.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            3666..4280
                     /gene="evgA"
                     /locus_tag="JN331_002310"
     CDS             3666..4280
                     /gene="evgA"
                     /locus_tag="JN331_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY7260441.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            4285..7878
                     /gene="evgS"
                     /locus_tag="JN331_002311"
     CDS             4285..7878
                     /gene="evgS"
                     /locus_tag="JN331_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY7260442.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNNYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPSVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFKIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(7934..9079)
                     /gene="yfdE"
                     /locus_tag="JN331_002312"
     CDS             complement(7934..9079)
                     /gene="yfdE"
                     /locus_tag="JN331_002312"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY7260443.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9153..10097)
                     /gene="yfdV"
                     /locus_tag="JN331_002313"
     CDS             complement(9153..10097)
                     /gene="yfdV"
                     /locus_tag="JN331_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY7260444.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLEPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10167..11861)
                     /gene="oxc"
                     /locus_tag="JN331_002314"
     CDS             complement(10167..11861)
                     /gene="oxc"
                     /locus_tag="JN331_002314"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY7260445.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(11915..13165)
                     /gene="frc"
                     /locus_tag="JN331_002315"
     CDS             complement(11915..13165)
                     /gene="frc"
                     /locus_tag="JN331_002315"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY7260446.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(13678..14301)
                     /locus_tag="JN331_002316"
     CDS             complement(13678..14301)
                     /locus_tag="JN331_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY7260447.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            14598..14873
                     /locus_tag="JN331_002317"
     CDS             14598..14873
                     /locus_tag="JN331_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260448.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(14950..15192)
                     /locus_tag="JN331_002318"
     CDS             complement(14950..15192)
                     /locus_tag="JN331_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY7260449.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            15545..16465
                     /gene="lpxP"
                     /locus_tag="JN331_002319"
     CDS             15545..16465
                     /gene="lpxP"
                     /locus_tag="JN331_002319"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY7260450.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMLGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTIVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            16820..16891
                     /gene="ypdK"
                     /locus_tag="JN331_002320"
     CDS             16820..16891
                     /gene="ypdK"
                     /locus_tag="JN331_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY7260451.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(16956..18194)
                     /gene="alaC"
                     /locus_tag="JN331_002321"
     CDS             complement(16956..18194)
                     /gene="alaC"
                     /locus_tag="JN331_002321"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY7260452.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            18571..>19838
                     /locus_tag="JN331_002322"
     CDS             18571..>19838
                     /locus_tag="JN331_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY7260453.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQ"
BASE COUNT         5628 a         4591 c         4220 g         5399 t
ORIGIN      
        1 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
       61 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
      121 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
      181 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
      241 caggaagaag gtattcgtct tgaaccttca gcactggcgg gtatggccgg acctcagcgc
      301 gtgtgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca attacgtaat
      361 gccactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
      421 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctaggtg
      481 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
      541 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
      601 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
      661 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accatagtat
      721 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
      781 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
      841 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
      901 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
      961 ccctggaaaa actgtggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     1021 gaacgccagt aatagcaaat cgcatacatc aaaaaactga atgtcactaa cacccgcatg
     1081 tctattttat tgccataacg tcctatcaaa ggtgaaatca atagtggcat gatgccgatg
     1141 ggcgcataag caagcccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     1201 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc aggtgatact cacaatacca
     1261 atggtgaaat tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     1321 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     1381 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     1441 ccgagcacta acagggtcag tcctggtaga ttcattttta ccggtgaagt ttcagtttct
     1501 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     1561 aaaaatatcc aaccccagct aaagttatcg caaatatagc cgcccaatat cggcccacat
     1621 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggt
     1681 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaatccc
     1741 tgaacgactc taaaaaatat cagcacatca agattggtcg ataaactaca cattaaggaa
     1801 gacagcgaaa aaaaagtgac tgaaagtaaa aataacctta attcgcctat tctttgtgcc
     1861 agcctgccgg taacagggat cgcaatggca tttgctacgc caaacgaggt gataacccag
     1921 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     1981 atagtggaat ccaacatttg cataaacgtc gctaatgaca atgcaatagt aacgcaccat
     2041 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     2101 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     2161 tactggtata agcaggcatg gaggtcacgg ttgaagccag atcaggcatc tcagcaatgt
     2221 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     2281 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     2341 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     2401 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     2461 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     2521 gacgtgccgg tacgacagcc attaacgatt gtccggaact cactgtttcg ccgacctgaa
     2581 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcacca
     2641 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     2701 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     2761 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     2821 tcgctaaagg cacacgacgg ttataatctt ctaaagattg ttgatactga atacgtgctg
     2881 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     2941 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     3001 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     3061 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     3121 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     3181 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     3241 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     3301 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     3361 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     3421 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
     3481 catcggtata atccctgatt ttattgttga catttcattt atgccgacta tttatatggt
     3541 atacttgtcg aattatctta aaggaagctc agattttctt atttttattg agaaaatgag
     3601 atgacgcctt atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat
     3661 aatctatgaa cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt
     3721 tattgatcaa aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc
     3781 agcgggtgga aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta
     3841 acggtatcca ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg
     3901 tctccgctaa aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt
     3961 tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg
     4021 gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc
     4081 aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca
     4141 aggataataa tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacgtata
     4201 aaagtcgcct gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcgc
     4261 aacgtaacaa aatcggctaa tcacatgaag tttttaccct atttttttct tctctgttgt
     4321 ggtctttggt cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt
     4381 agtaacaacc gtgtcacact tgatccagta cgtctgagca acaaggaatt acgttggtta
     4441 gcgagcaaaa aaaatcttgt gattgcagta cacaagtccc aaacggctac cttgttgcat
     4501 accgattcgc agcaacaggt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga
     4561 gcgttaaata tcaaattaac actccgggaa tacgcagatc atcaaaaagc gatggacgcg
     4621 cttgcagaag gtgaagtcga tatagtgtta tcacatttag ttacttcgcc gcctcttaat
     4681 aatgacattg ctgcaaccaa accattgata attacctttc cggcgctggt aaccaccctt
     4741 cacgactcaa tgcgaccgct tacctcacca aaaccagtaa atattgctcg ggtagcaaat
     4801 taccccccag acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca
     4861 aatttatatc aggcattagc atccgtctca gctgggcaca ataattactt tattggtagt
     4921 aacatcatta ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa
     4981 tattataact cgccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcattctt
     5041 aatgaagtac tcaatagatt tgttgatgct ttaacaaatg aagttcgcta tgaagtatca
     5101 caaaattggc ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa
     5161 catgaaaaac agtggattaa gcagcatccc aatttaaagg tgctggaaaa tccttactcg
     5221 cccccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggacattctt
     5281 aatattatta ccttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
     5341 catgctggaa cacagcttag ccccggagga tgggatataa tacctggcgc tatttatagt
     5401 gaagatcgag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
     5461 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
     5521 ccatattatt atgagctgca ttcgcaatta aaagagatgt atccggaggt tgaatggata
     5581 caggtcgata atgccagcgc tgcatttcac aaggttaagg aaggtgaact tgatgctctg
     5641 gtcgcgacac agctaaattc gcgttacatg atcgatcatt actatcctaa tgaactttat
     5701 cattttctta ttcctagcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
     5761 ccggaactta aggatattat taataaagca ctgaatgcaa ttcccccaag cgaagttctg
     5821 cgcctgacgg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctatat
     5881 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
     5941 tggggattct acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
     6001 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
     6061 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
     6121 tactacaaaa atgcaatgtt accattagaa aacagtgact caccctttaa agatgttttt
     6181 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggta
     6241 tttaaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
     6301 cttcctgcaa gtgacaatgc agtatatatt tgtggttggc aagatattac tgaaacgcgt
     6361 gatctaatta atgcactcga ggtagaaaaa aataaagcga taaaggctac cgtagcaaaa
     6421 agtcagtttc tggcaacgat gagtcacgaa ataagaacac caataagctc tattatgggc
     6481 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
     6541 cttgcctacg ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
     6601 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
     6661 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaagttgc
     6721 agcagtacat ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc
     6781 ttatcaaatt tactgagtaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
     6841 acctccctgg gtcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
     6901 ggaagtggat tatcgcagga agaacaacaa caactgttta aacgctacag ccaaacaagt
     6961 gcaggtcgtc agcaaacagg ttctggttta ggcttaatga tctgcaaaga attaattaaa
     7021 aatatgcagg gcgatttatc attagaaagt catccaggca taggaacaac atttacgatc
     7081 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacaaccc
     7141 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
     7201 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
     7261 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
     7321 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
     7381 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
     7441 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
     7501 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
     7561 aataatacgg cgaatgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
     7621 acgcataaag atctacccgc tgcgtttcat gcactagaag ctggcgataa cagaactttc
     7681 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
     7741 aatattagcc atcagttaga aataacacct gcttcagatg acagtaagcc tgaaattctt
     7801 cagctgctga actctgttaa agaacacatt gcagagctgg accaggagat tgctgttttc
     7861 tgtcagaaaa atgactaaat tgcggctccc acaatgttct aatgtgggag ctatttacca
     7921 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
     7981 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
     8041 gcatcattat tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa
     8101 tggcctcagc cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta
     8161 accaaacttc agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct
     8221 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
     8281 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
     8341 tatcctgagt atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt
     8401 gtggtgactt ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
     8461 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
     8521 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa
     8581 cacgcactgg cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa
     8641 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
     8701 aagcatatat gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt
     8761 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
     8821 atatactttt atcatgatca ttctttaaat caagaaccac actctctttg ccatgattaa
     8881 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
     8941 catgacctgg tggctcaact ttaattaccc ttgcgcccat attataaaga agttgagttc
     9001 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
     9061 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
     9121 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
     9181 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
     9241 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
     9301 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
     9361 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
     9421 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
     9481 gccttcgcaa tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccagc
     9541 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
     9601 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
     9661 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
     9721 gatacggaat caccataaat aggctcgaga actgcaaacc ccaagaatcc aatggtaggt
     9781 gaacccgcaa ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag
     9841 caaccaaacc aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
     9901 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
     9961 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    10021 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    10081 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    10141 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    10201 gtaatatggc cactttcagt tcctgctgca gggtcgatga ccacattaat aatggtcggt
    10261 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    10321 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    10381 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    10441 ttattaaaaa taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc
    10501 ccactgaaac caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg
    10561 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    10621 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    10681 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    10741 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    10801 tgctgcttgt ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt
    10861 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    10921 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    10981 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    11041 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    11101 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    11161 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    11221 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    11281 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    11341 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    11401 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    11461 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    11521 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    11581 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    11641 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    11701 tcatgacgaa aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc
    11761 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    11821 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    11881 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    11941 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    12001 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    12061 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    12121 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    12181 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    12241 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc
    12301 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    12361 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    12421 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    12481 gcgggtattc ttccagataa cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    12541 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    12601 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    12661 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    12721 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    12781 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    12841 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    12901 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    12961 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    13021 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    13081 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    13141 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    13201 ttagcgttta tttaaggcat aacgccctct ataaataatc tgcaaaatat cttcgtagcg
    13261 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gccacttcca
    13321 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tattgattcg
    13381 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    13441 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    13501 taaatgattt gtgatgacac gggattattg cggtttactt tgaggaaaat aaaatgcgtg
    13561 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    13621 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    13681 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    13741 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    13801 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    13861 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    13921 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    13981 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    14041 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    14101 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    14161 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    14221 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    14281 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    14341 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    14401 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgct
    14461 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    14521 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    14581 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    14641 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    14701 ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat
    14761 cacaggcgat tttgtttaag atgagagaag atgttccacc attaaacctc acagaagaaa
    14821 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt
    14881 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    14941 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    15001 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    15061 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    15121 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    15181 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    15241 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    15301 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    15361 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    15421 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    15481 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    15541 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    15601 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    15661 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    15721 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    15781 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    15841 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
    15901 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    15961 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    16021 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgctcggcag
    16081 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    16141 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    16201 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    16261 aacgatagta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga
    16321 aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga
    16381 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    16441 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    16501 cataataacc tcatgatttt tatggctttt ttcttaagtt ttttccttcg ctgaatatct
    16561 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    16621 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    16681 gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacagggct tttaaggtcg
    16741 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    16801 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggttatcgt
    16861 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    16921 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    16981 ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    17041 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    17101 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    17161 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    17221 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    17281 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    17341 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    17401 gcactgacca gcgttttgtt gccgaccata aagccgatac gccagcccgc catgttgtag
    17461 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    17521 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    17581 acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    17641 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    17701 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    17761 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    17821 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    17881 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    17941 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    18001 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    18061 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    18121 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    18181 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    18241 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    18301 aacattttta tcaatggctt taaatgagga actccacttc aaaaacagga aatttttcta
    18361 cgctaacaag aaaattacca ccaattttat taacattata atttcaataa atttaccagt
    18421 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    18481 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    18541 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    18601 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    18661 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    18721 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    18781 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    18841 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    18901 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    18961 aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt gtgtgagacg
    19021 ttgaccatga ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc
    19081 tctaaaatcg gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgcttctg
    19141 gtgcaaagcg ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg
    19201 gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag
    19261 gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat
    19321 catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt
    19381 agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa
    19441 gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc
    19501 gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag
    19561 gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag
    19621 cagctacgtg aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc
    19681 cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc
    19741 gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac
    19801 gatgagcaaa tcgatatcaa aaaagagctg tatcaaat
//
LOCUS       DADLSB010000069        19697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.069, whole genome shotgun sequence.
ACCESSION   DADLSB010000069 DADLSB010000000
VERSION     DADLSB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.069"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="JN331_002323"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="JN331_002323"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY7260454.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1542..2348
                     /gene="tcdA"
                     /locus_tag="JN331_002324"
     CDS             1542..2348
                     /gene="tcdA"
                     /locus_tag="JN331_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY7260455.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2399..2842)
                     /gene="csdE"
                     /locus_tag="JN331_002325"
     CDS             complement(2399..2842)
                     /gene="csdE"
                     /locus_tag="JN331_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY7260456.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2842..4047)
                     /gene="csdA"
                     /locus_tag="JN331_002326"
     CDS             complement(2842..4047)
                     /gene="csdA"
                     /locus_tag="JN331_002326"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY7260457.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4239..4466
                     /locus_tag="JN331_002327"
     CDS             4239..4466
                     /locus_tag="JN331_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY7260458.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4817..5734
                     /locus_tag="JN331_002328"
     CDS             4817..5734
                     /locus_tag="JN331_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY7260459.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5753..6148
                     /locus_tag="JN331_002329"
     CDS             5753..6148
                     /locus_tag="JN331_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY7260460.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6141..7241
                     /gene="rlmM"
                     /locus_tag="JN331_002330"
     CDS             6141..7241
                     /gene="rlmM"
                     /locus_tag="JN331_002330"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY7260461.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7285..8016)
                     /gene="fucR"
                     /locus_tag="JN331_002331"
     CDS             complement(7285..8016)
                     /gene="fucR"
                     /locus_tag="JN331_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY7260462.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8074..8496)
                     /gene="fucU"
                     /locus_tag="JN331_002332"
     CDS             complement(8074..8496)
                     /gene="fucU"
                     /locus_tag="JN331_002332"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY7260463.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8498..9916)
                     /gene="fucK"
                     /locus_tag="JN331_002333"
     CDS             complement(8498..9916)
                     /gene="fucK"
                     /locus_tag="JN331_002333"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HAY7260464.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVNKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAWLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     YELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JN331_002334"
                     /pseudo
     CDS             complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JN331_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JN331_002335"
     CDS             complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JN331_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY7260465.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13696..14343
                     /gene="fucA"
                     /locus_tag="JN331_002336"
     CDS             13696..14343
                     /gene="fucA"
                     /locus_tag="JN331_002336"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY7260466.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14371..15519
                     /gene="fucO"
                     /locus_tag="JN331_002337"
     CDS             14371..15519
                     /gene="fucO"
                     /locus_tag="JN331_002337"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY7260467.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JN331_002338"
     CDS             complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JN331_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY7260468.1"
                     /translation="MRGLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JN331_002339"
     CDS             complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JN331_002339"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY7260469.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17863..19152)
                     /locus_tag="JN331_002340"
     CDS             complement(17863..19152)
                     /locus_tag="JN331_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7260470.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4865 a         4826 c         5196 g         4810 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaaagcc tattttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgccgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgcgg gggcattggg cgataaatac atcctcgctt acagcaactc
      721 tctgatggat aacttcatta tggatgtgca gggtagtggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcattggggc gaaacacaca gtgaagccga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ttggatgtcg ggggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac gggtttgggt
     1261 gctgaaaacg gctccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc
     1321 cgtttttacg gatatgacag ggtgaggggt aacctcacat cctgattatt gactaagtgc
     1381 gcgttgttca taccggatgc ggcgtgaacg ccttatccgg cctacaaatc atgcaaatcc
     1441 aaaatattgc aggagttatg taggcctgat aagcgtagcg catcaggcat ttattcgttt
     1501 agggtgaggg ataacctcgc cctttttaat tctgaggtgc tatgtctgtg gtaattagtg
     1561 atgcatggcg tcagcgtttt ggtggcacag cgcgtctgta tggtgaaaaa gcgttgcaac
     1621 tgtttgctga cgcgcatatt tgtgtggttg gtatcggtgg tgtcggttcc tgggcggcgg
     1681 aagcgctggc gcgcacgggg attggcgcaa tcacgcttat cgatatggat gatgtgtgcg
     1741 tcaccaatac caatcggcaa attcacgctc tgcgcgataa cgtcgggctg gcaaaagcgg
     1801 aagtcatggc ggagcgcatt cgccagatta acccggagtg ccgtgtaacg gtggtggatg
     1861 atttcgtgac gccggataac gtagcgcaat atatgagtgc cggttattcg tacgtgattg
     1921 atgccattga tagtgtacgg cccaaagcgg cgctgattgc ttattgtcgg cgcaataaaa
     1981 tcccgctggt tacgaccggt ggcgcgggtg ggcagattga cccgacgcag attcaggtta
     2041 ccgatctggc gaaaacgatt caggatccgc tggcggcgaa gttgcgcgag cgcctgaaaa
     2101 gcgattttgg cgtagtgaaa aacagtaaag gtaagctcgg cgtggattgc gtgttttcta
     2161 ctgaagcgct ggtgtacccg cagtcagacg gtacggtgtg tgcgatgaaa gccacggcag
     2221 aagggccgaa gcggatggat tgtgcatctg gatttggcgc ggcaacgatg gtgaccgcca
     2281 cctttgggtt tgttgcggtt tctcatgcgc tgaagaagat gatggcgaaa gcggcgcgtc
     2341 agggttaaac aatcaggtcg gatgagacgc gaacgtctta tccgacccgg ttctgtgctt
     2401 aaacctgctt cgtcgcagcg ataatcgcct cgcttaacgc atttaacccc tggctgcgtg
     2461 aggcgctaag ctgcgcacgt aatcccagct catcaaacaa tgccagcggc gactgtgcct
     2521 gtaattcggc ggcggttttc ccctcaacgg cagtcaacaa caccgccaac aggccgcgca
     2581 caatgcgccc ttcgctgtcg ccaaagaagt gcattttgcc gttttcagcc acggtatatc
     2641 ccagccagac gcggttttcg catccggcaa tctctttagc ctgcgctttt aactcgtctg
     2701 gcaaagccgg aagctgtttc cccagcatga tcaactggcg atatttatct tcccattgcg
     2761 acaacggtgc gaaggtattg cgtaacgttt ctgcggttac ggttgtgccg aacggatgtc
     2821 cggcgaattg cgggtttgtc attaatccac caataattcc agcgcgcggt caacggcatt
     2881 caccagcgca tccacatcac tctttgtatt atatggcgca aatgaggcgc gcagtgtgcc
     2941 ggttacgcct aattctgcca gtagcggctg agcgcaatgt tgcccggccc gcaaggcaat
     3001 accgtactcc gccagtagcg tcaccatatc gctgtgatga acgccagcaa aatcaaaggc
     3061 cagcaggctg gaatcctggc agcggaatga acgaaagcct ggacgtttcg ccagcgcttc
     3121 ttctgcaagc gttgctaagc tacggctcca gctttcggcc tggttgatat cgtaatctgc
     3181 cagccattcc agcgccgcgc ttaatcctat gacgccagcg acatttggcg ttcctgcttc
     3241 cagtttccac ggcgcagatt gagtggtgaa accgtcaaaa ctcacttcgt gaatcatttt
     3301 gccgccaccc agccagggcg acatcgcttc cagcagttct gatttgccat acagcacgcc
     3361 gatacccgtc gggccataca gtttgtgacc agagaaagca tagaaatcga tatccagttg
     3421 ctgaacatcc gcggggaaat gcaccgcccc ctgagcacca tcaaccatca ccaccatccc
     3481 ggctgaatga gcaaaggtaa tcgctcgcgc cagatccggg caaccgccag tcacgttcga
     3541 catctgaccc aacgccagaa tccgactacg gggagtaatc agttctggca acaaatcgac
     3601 atccggcagt cgctgcgcat taagcggcaa tttcaccact ttggctctag tttgttgggc
     3661 gaccatcagc caggggacga ggttagcgtg gtgttctgcc acgctgacaa taatctcatc
     3721 gcccggttgc agacgcggac gcgcatagca ttgtgccacc atgttgatgg attcagtggt
     3781 gccgcgcgtc cagacgatag ttttatcatc cggtgcattc agtaattgcg ccactttctc
     3841 tcgtgcagct tcataacgcg cggtcaggcg ttgggcttcg gcaaactggc tgcgatggac
     3901 gtttccggcg ctcaggctgt aaaactgtcg agtggcttca accacggctt caggtttaag
     3961 cgcggtcgcg gcgctgtcga gatagacgcc cgcatcctgt agtgcgggaa actgggcgcg
     4021 aaactgcgcg ggattaaaaa cgttcatggt actcctcggc ttgatgcacc gatcgtgacg
     4081 caaaattcac tttgatacaa ggagagtgct aaccatcaga attatctgga tatcctggcg
     4141 aagccgcgta agtaacatat gctgaatagt gttaggcgtt aattttagcc tgttaatcaa
     4201 tactattaag atttattaat cgctttaagg agaagatgat gaaaaagact gccgcaatta
     4261 tttctgcctg tatgctgact tttgccctga gcgcctgttc cggttcgaac tatgtgatgc
     4321 acaccaatga cggacgtacc atcgtctctg acggcaaacc acagactgat aacgataccg
     4381 gtatgatttc gtataaagac gctaatggca acaaacagca gatcaaccgt actgacgtga
     4441 aagagatggt cgaactggaa cagtaattcg cgatcgcaag gtaaaaaaaa gcaccgcaat
     4501 taggcggtgc tacattaatc actatggaca gacagggtaa atgtacagga agtgaaaaaa
     4561 ggtagctttg ctaccatggt ctgaatcgca gaccaattgc aaacgcaaca acacaacatc
     4621 acaaccgtaa gccaaaagtt caccagaata cgcattccga taaaactttt cgttccggct
     4681 caggaagtgc cgccactata ggtatttgct ggtagaagct caacggacaa tttataatgg
     4741 ctcagattaa aaaaactaat aggttacaca gtgtgatcta attgttaaat tcatttaaca
     4801 tcaaagttta aaagccatgt ctaaacgatt accaccgcta aatgccttac gagtttttga
     4861 tgccgcagca cgccatttaa gtttcactcg cgcagcagaa gagctttttg tgacccaggc
     4921 cgcagtaagt catcaaatca agtctcttga ggattttttg gggctaaaac tgttccgccg
     4981 ccgtaatcgt tcactcctgc tgaccgagga agggcaaagc tatttcctcg atatcaaaga
     5041 gatattttcg caattaaccg aagcgacgcg taaactccag gcccgtagcg ccaagggggc
     5101 gttgacggtc agtttactcc ccagtttcgc cattcattgg ttggttccgc gactttccag
     5161 ctttaattca gcttatccgg gaattgacgt tcgaatccag gcggttgatc gtcaggaaga
     5221 taagctggcg gatgatgttg atgtggcgat attttatggt cggggcaact ggccggggct
     5281 acgggtggaa aaactgtacg ccgaatattt attgccggtg tgttcgccgc tactgctgac
     5341 aggcgaaaaa cccttgaaga ccccggaaga tctggctaaa catacgttat tacatgatgc
     5401 gtcacgccgt gactggcaga catatacccg acagttgggg ttaaatcata tcaacgttca
     5461 gcaagggcca atttttagtc atagcgccat ggtgctgcaa gcggctattc acgggcaggg
     5521 agtggcgctg gcaaataacg tgatggcgca atctgaaatc gaggccggac gtcttgtttg
     5581 cccgtttaat gatgttctgg tcagtaaaaa tgctttttat ctggtttgtc atgacagcca
     5641 ggcagaactg ggtaaaatag ccgcctttcg ccaatggatc ctggcgaaag ccgctgctga
     5701 acaagaaaaa ttccgctttc gttatgaaca ataatttacg tagggtacga ccatgaccag
     5761 ccgttttatg ctgattttcg ccgccattag cggcttcatt tttgtggcac tgggcgcttt
     5821 tggtgcgcat gtgttaagta aaaccatggg ggccgttgag atgggctgga tccaaaccgg
     5881 tctcgaatac caggcgtttc atacgctggc gatcttaggt ctggcggtgg caatgcagcg
     5941 tcgcatcagt atctggtttt actggagtag cgttttcctc gcgttaggca cggtgctgtt
     6001 cagcggcagc ctttattgcc tggcgctgtc ccatctgcgt ttgtgggcgt ttgtcacacc
     6061 ggttggcggc gtgagcttcc ttgcgggctg ggcgttaatg ttagttggtg ctatccgttt
     6121 aaagcgcaag ggcgtaagtc atgaataagg ttgtattgct gtgccgtccg ggctttgaaa
     6181 aagagtgcgc cgcagaaatt accgataaag ccggccagcg ggaaattttc ggttttgccc
     6241 gcgtgaaaga gaatgcgggt tatgtcattt atgaatgtta tcaacctgat gatggcgata
     6301 agttaatccg tgagctgccg ttcagttcat taatttttgc ccgccagtgg tttgtggtgg
     6361 gggaactcct gcagcatttg ccgccagaag atcgtattac ccccattgtc ggcatgttac
     6421 agggtgtagt agagaagggc ggtgaactgc gtgttgaagt tgccgatacc aacgaaagca
     6481 aagagttgct gaaattctgc cgtaagttca ctgttccgct acgcgctgcc ttgcgcgatg
     6541 ctggggtgct ggcgaactat gaaacgccga agcgtccggt tgtgcatgta ttctttattg
     6601 caccaggatg ctgctatacc ggttactcat acagcaacaa taattcgccg ttctatatgg
     6661 gcattccgcg cctgaaattt ccggcagagg cgccgagtcg ttccacgctc aaactggaag
     6721 aggcatttca tgtgtttatt cctgcggatg agtgggatga acgcctggcg aacgggatgt
     6781 gggcggtgga tttaggcgct tgccctggcg gctggaccta ccaactggtg aagcgcaaca
     6841 tgtgggttta ttccgtcgac aacggcccga tggcgcaaag tctgatggat accgggcagg
     6901 tgacgtggct gcgggaagac ggtttcaaat tccgtccgac acgcagcaat atctcctgga
     6961 tggtatgcga tatggttgaa aaaccggcta aagttgcggc attgatggcg cagtggctgg
     7021 ttaatggctg gtgccgtgaa accattttca atctcaaact gccgatgaaa aaacgctacg
     7081 aagaagtatc acacaatctg gcgtatattc aggcacagct tgatgaacat ggcataaatg
     7141 ctcagattca ggcgcggcag ttgtatcacg atcgcgaaga agtgacggtg cacgtccgcc
     7201 gcatctgggc tgcggtgggt ggtcgtcgcg acgagcgata acaggcaagg ccggggcgtc
     7261 gtctccggcc tgctaccctt tccgtcaggc tgttaccaaa gaagttgcaa cctggcgctc
     7321 atcagaaata atgtgtgtta cctcatccag atgcccgatg cgggcttccc ctgaacgatt
     7381 aaatttactt ttatcaatca ataacaacga ttgcgcggca cgttttaata gcatcgattt
     7441 gtaatcagcg ttgatcgcat tggagtccca cagtgcgccg ctgctatcga tcccttcaca
     7501 agaaaaaata aacagatcga tttccagcga tttaagttgg gaaatcagcg agggattgac
     7561 gtaacagcca tatttgcgct caagtgtgcc gccggaactg atcagttgaa tgcgttcgcg
     7621 tttaccgagt tcatggcaaa tcggatggct attggtgaag acctgaatgt tgatgtcagg
     7681 caactggcgt gccagatacc agcaagttga actggcgtct aaggctatca ccatcccttc
     7741 ttcaatccac gcgagcgcct cgcgcgcgat atctgcttta tgcgcataat ggcttttcag
     7801 cctgatgtga aagggatcgc cactgtcttg attttgacgg tggatatatt tagcgcgtcc
     7861 atgattgcgc agaattttac cctgcgtctg taattcattg agatcgcgac gaatggtttc
     7921 tttactgacc tttagctgtt cagagagagc ttccgtggtc aggctggtat ggttcagcag
     7981 caggtcgact atcgcttgct ggcgtgccgc tttcatattt ttcaccccat cattcgataa
     8041 ccgcccgcgt catgcgggcg taccggtatg agattacggt gttacccctt tttttaaaag
     8101 aatattcccg tacttcgctc gttcgcctgt gataacgatc gcaaaggctt tttgcgcccg
     8161 ttcataaaac gcaaaacgat tgatgcggat gatgtcagga cacggggctt gtagtgaaag
     8221 cgcattacgg taacgtcgtt ctacttcagg atcgagagtg tcaccctcta ccgccgccat
     8281 catcaccagc ggcggggcat aactgtccag ctcaaataac gggataatcg cctggagcaa
     8341 gtcgctcacc aacaggccat cagcgcggat cacctgcggt cccatcgaat gggcgggaaa
     8401 gtgagcatcg gaaaaaataa tttcatctcc atgtcccatc tctgccagca ctttcaatag
     8461 ttcgggagaa attaacggcg aaattgtttt cagcatttca cacttcctct ataaattcag
     8521 gttcagtttg cgggtagaaa taacggtact gataatgaat ctgtgcacgg gcttcttccg
     8581 ggctgttaaa ttcccctacg ccataccaac cgaacagcgc agctcctgcg acggtcgttt
     8641 cggcatcgtc gagaactttt accggaatat caagcatatt ggctttaatc tgattccaca
     8701 atgtgttgcg acttcctcca ccgactaaca ataattcata ggctttaaag tgcccgattt
     8761 tttccagcat ctgtagattg cgctgtaatt gcgcagttaa cccttccagc gccgcgcgat
     8821 agaaatgccc ccgcgtggta ttaagcgtca ctccttgcca gccagcgttc tgacacgaca
     8881 ataaatcaca ctgcattttc acaccatccg cgccaggtgc gatcagacga gcttcttcaa
     8941 tcaacatttg ccagggtgtt tcagccgtcc agaacagttt tctcacccat tccagcacgc
     9001 cggatgccag ccattgcata cctgggttat acaaccatgc ctggctatct agttcgcagg
     9061 tggaaccggc gtactgactt aacagcgaag tatcaacctg ggcgctgcga accattaaaa
     9121 tttcccatgt accggaggag agcacgggtt cattttgttc ggcaccagcg ccaaaaaggg
     9181 cgaactgggt atcgtgacct gcggaaatca ccggtatgcc aacgggtaag ccgagcattg
     9241 ctgcggcgct gttctgtagc gtaccaatct gttcacccgc ttccaccaga cgagggaaga
     9301 gtcggcgtgg aataccggtg gcttgtaaaa tttgcggact gaaatcgcgt tgctggatat
     9361 ccagcatctg gctggttccg gccatcgtga tatcagtagt gaattcgccg gttaaacggt
     9421 ggttaatcag cgacgaaatg aagagccagg cgtgcgcgcg ttccagcagt tgtggatgat
     9481 tttctttcaa ccacaccaac ttatataacg tattgaaact aaaggctccg acgccagaaa
     9541 tagcctgcaa ccgctgtgcg gagattaacc gttcaatatt gtccataacc gctgctgttc
     9601 gcggacattt ccagctaatg atcggataga gcagattgcc ttgcttattt accagagcgc
     9661 catccacacc aaaggtggtg acggcgatac cgcggatgtg gcattcagtc agttcactat
     9721 tgatttgccg acagcaatca gcaaagcgtt gcaaaatggc gtctaaagac cactggtgcc
     9781 aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg gcaacaattt
     9841 ttccctgccg attaaccgcg atggccctga cattggtcgc gccacagtcg agtaccagga
     9901 taacttcttg tttcataatc gctcctgcaa tatagccgga taacattgtt tatccggcta
     9961 accactcttg cttgttcagg cccggtagcc ggagcgaccg ggcatcacat cagggagtaa
    10021 tgtattaacg cttgtacaac ggaccgtagt tctggcaagc gcggtaatcc tggccttcaa
    10081 tatccatgcc gtgcgcagcc caggcagaag gacgatacac tttggtctct tcaacgttgt
    10141 gcatacatac cgggatacgc agcatggagg caagagtgat aaagtctgcg ccaacgtggc
    10201 cgatggtcag aactccgtgg ttggcacccc agttcgccat taccgaatac acatccgtaa
    10261 acggcccttt accagtgagg cgcggtgcaa accaggtggt tggctaggtt gagttggtgc
    10321 gtttgttgag gatgtcatgc acatccttcg gcaattccac gctccagcct tccgcgattt
    10381 gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt catggtgaac gggacgccgc
    10441 cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc
    10501 accattcggt agcggcgagg caagcgtcag cctcttgctg agagatttcc cagtgcggct
    10561 tcatcgtcgg gttaccttcg ctgtcgcgtt gtttacagga accgtccagc gcagcagaac
    10621 ctgagttgat caaatggatg atgccgtgtt ctgccagtcc gtccagttta tgccccgtta
    10681 cacgctcaat tgcttctggt gaccagtagg tacgcacatc ggcaaatacc tgagcggtgc
    10741 cggtgagctg gtgacccatt agcattgcca cgccgttaag actgtcgttt tcggtcgcca
    10801 cgacaaaggg ttcgcgcacg ccattccagt caaatgaact gttgaggatc gcttcggcgg
    10861 tgtcaccatt gggatattga tcggtccagt gacgttgccc ctggaagccc gcagcgatgg
    10921 cgttgtagcc aagtgattct tccacgcgac caatatcggc cagtttgctg ttgccttgca
    10981 tcatgtcgcg gatacacatc gccatcagta aactttcgcg cagaactgcg cggctttgct
    11041 cggcattgcg ttgatactgt ttgttatttt catcttcgcc atagcggaag tttttatcag
    11101 cccaggccag tgccatttcc agttcggctt cgtcgtaaat cttctgatcg atacggcgac
    11161 gcagttcggt catatccacc gcctggactt tcattcccag ccaggattca aagaagttgt
    11221 gatcaacaat agaaccggcg atacccatcg aaacgccgcc cagcgacaga tagcttttac
    11281 ctttcatgct ggcgacggcc aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat
    11341 cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg gagaatgccg
    11401 ggatgccttt ctggctgtga gctgccagag ccgctgccag gtaaacagcg ccggggcgtt
    11461 cagtgccgtt aaagccccaa atggctttcg ggcgggttgg atccatgtcg atggtttcac
    11521 tgccatagca ccagcaaggc gttaccgtaa tggtgaggcc tacattctgg ctgctgaatt
    11581 tttcttcgca agcagcggct tcagccatac ccgcgataca ggtatcggaa atgacacact
    11641 cgacggcagc tccgcaggca tggcgcagtt tctcggtcag cagtgcggcc gtagctttcg
    11701 ccatattcat tgtttgttct tcaagcgact cacgaacacc catgcgacga ccgtcaataa
    11761 ccgggcggat accaattttc ggtaagctga tttttttcat tacagattcc tcactttatt
    11821 cggaaaatat gttcagttag ttgccgtttg agaacggaaa cgggcaaaga taaagatgac
    11881 cgcgaagcag agtgcgggga tcagttcagc agtggggatg ttgcccgccg cgtcactgac
    11941 aaaacccatg accggagtga caataccgcc gccaataatg gtcataacga tgaaggacga
    12001 accgtatttg gtgtcctggc cgagattctt aatgcccagc gagaagattg ttgggtactg
    12061 aatcgacata aaggcgctgc ataaagtcag ggctattaag cccacatgac cgccagcgaa
    12121 ggctgagatc aggcacagtg ccatagcgat taatgcgtag gcggccagga ctttgtgtgg
    12181 tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca ataaagaagc acaccatggt
    12241 tccggttaaa tagttagcgg caaagcctgc agtcatacct ggaatttctt ctacagcgta
    12301 gcgaatcaaa tagctccagc aggccgtttg tgcgccgaca tagcagaatt gcgctaatac
    12361 cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc gatgcggaga acgatccttg
    12421 tttggcgtca ctgtgattat cactctgcaa tgccgggaat ttcgtcagca tgatcagcag
    12481 ggcgaccagt aacacgatag ccacgatgat catataaggt gtctgtaccg ataataccag
    12541 gctgtgttta tacgcactca attgctctgg agacatttta tcgagaacgt cttgcgattg
    12601 atgtggcacg ttagacaaaa taagactttg cccaaagaca accgcgataa ttgcgccaaa
    12661 cgagttaaat gtttgcgcaa gatttaagcg gaagtgacta ctgctttccg gccctaatac
    12721 cgtaacaaaa gggtttgcgg cagtttccag acaacctaat ccggctgcaa taataaatag
    12781 gccaactaaa aataaggtgt agttcattat ttctgcagcg ggccagaata atgcagcacc
    12841 caaggcatat aaaaataatc cggtaataat ccctgcttta taactgagtt ttttcatcaa
    12901 tatcccagca gggattggga taatgaaata accaaagtaa aaggccgatt ggatcaggcc
    12961 agcctggaaa tttgtcagcg taaaagcctg ctggaattga ggtaataaaa tgtcgttaag
    13021 gttattggct accgcccaaa gaaaaaacag tgagcacagc agcgcgaatg gaataatgta
    13081 acttctgctt tgccctgcat ctttatctac cgcacggtaa ctctgcgttt gtattgatgt
    13141 gtttcccata gcatcctctt aggttcagaa gcttaagcac gtaatcggtt atgttttcaa
    13201 aagtgcaaaa ccagcgagtt cgggcttcat atcggtgaat atttgcctgt ctgaaatgaa
    13261 ctcatggatt cattttcaaa taaaaaacgc tctgtaatat gacggcggtc acacttatta
    13321 gctggaaggt gactttatgt gactaccatc actttaataa tcgaaatttc atgtccgttt
    13381 cagaaaataa atcataaaaa acacaaacca gatgaccgta ttaattgttt gtgccgaaaa
    13441 cgggcatttc tggcgagggg aaatggaaaa atgaccgttt tgataaatat ttttaagagc
    13501 ggtcattttt aacggggtta taaaaagaga acttgcagtt atttactgtg attagttgag
    13561 ctaggtcaca aaatataagc ttgttaattt ctttcttgtt aatcaggaag aagatggtgg
    13621 gtaattaaac ggctaattca atagtgtgaa aggaacaaca ttattgccct gttttgaatc
    13681 agagagaggt agctaatgga acgaaataaa cttgctcgtc agattattga cacttgcctg
    13741 gaaatgaccc gcctgggact gaaccagggg acagcgggga acgtcagtgt acgttatcag
    13801 gatgggatgc tgattacgcc tacaggcatt ccatatgaaa aactgacgga gtcgcatatt
    13861 gtctttattg atggcaacgg taaacatgag gaaggaaagc tcccctcaag cgaatggcgt
    13921 ttccatatgg cagcctatca aagcagaccg gatgccaacg cggttgttca caatcatgcc
    13981 gttcattgca cggcagtttc cattcttaac cgatcgatcc ccgctattca ctacatgatt
    14041 gcggcggctg gcggtaattc tattccttgc gcgccttatg cgacctttgg aacacgcgaa
    14101 ctttctgaac atgttgcgct ggctctcaaa aatcgtaagg caactttgtt acaacatcat
    14161 gggcttatcg cttgtgaggt gaatctggaa aaagcgttat ggctggcgca tgaagttgaa
    14221 gtgctggcgc aactttacct gacgaccctg gcgattacgg acccggtgcc agtgctgagc
    14281 gatgaagaga ttgccgtagt gctggagaaa ttcaaaacct atgggttacg gattgaagag
    14341 taatttcgta aagcaacaag gagaaggatg atggctaaca gaatgattct gaacgaaacg
    14401 gcatggtttg gtcggggtgc tgttggggct ttaaccgatg aggtgaaacg ccgtggttat
    14461 cagaaggcgc tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt ggcgaaagtg
    14521 accgataaga tggatgctgc agggctggca tgggcgattt acgacggcgt agtgcccaac
    14581 ccaacaatta ctgtcgtcaa agaagggctc ggtgtattcc agaatagcgg cgcggattac
    14641 ctgatcgcta ttggtggtgg ttctccacag gatacttgta aagcaattgg cattatcagc
    14701 aacaacccgg agtttgccga tgtgcgtagc ctggaagggc tttccccgac caataaaccc
    14761 agtgtaccga ttctggcaat ccccaccaca gcaggtactg cggcagaagt gaccattaac
    14821 tacgtgatca ctgacgaaga aaaacggcgc aagtttgttt gcgttgatcc gcatgatatc
    14881 ccgcaggtgg cgtttattga tgctgacatg atggatggta tgcctccggc gctgaaagcc
    14941 gcgacgggtg tcgatgcgct cactcatgct attgaggggt atattacccg tggcgcgtgg
    15001 gcactaaccg atgcactgca cattaaagcg attgaaatca ttgctggggc gctgcgagga
    15061 tcggttgctg gtgataagga tgccggagaa gaaatggcgc tcgggcagta tgttgcgggt
    15121 atgggcttct cgaatgttgg gttagggttg gtgcatggta tggcgcatcc actgggcgcg
    15181 ttttataaca ctccacacgg tgttgcgaac gccatcctgt taccgcatgt catgcgctat
    15241 aacgctgact ttaccggtga gaagtaccgc gatatcgcgc gcgttatggg cgtgaaagtg
    15301 gaaggtatga gcctggaagg ggcgcgtaat gccgctgttg aagcggtgtt tgccctcaac
    15361 cgtgatgtcg gtattccgcc acatttgcgt gatgttggtg tacgcaagga agacatttcg
    15421 gcactggctc aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg tgaagcaacg
    15481 cttgaggata ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg tgataatgcc
    15541 ggatacgatt tgcggcgtat ccggctcgcc gttaccgtac caaccgcaat tgctgtaaat
    15601 tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
    15661 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
    15721 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
    15781 caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct
    15841 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
    15901 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
    15961 tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag cgtggcggct aagtcatcgg
    16021 cttcgttgcc gctggctgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg
    16081 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg atccgctttg taatctggta
    16141 aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg accgcgtggg
    16201 ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg
    16261 aaccctgaac ggcatgaatg cgacggataa gattcagtgc atcgacaata agcaaatgaa
    16321 cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg ccggaacagg
    16381 ctatggctgg ttgagaaaac aggaagaggc ctcgcaaaac gaggcctctg ggagagatta
    16441 atcgcaggca acgatcttca tcgccaggcc gccgcgagag gtttcgcggt acttggcgtt
    16501 catgtcttta cctgtttcgt acatggtttc gataacttta tcgaggcaga cgcgcggttc
    16561 gctggtacgg cgcagtgcca tacgcgcggc gttcaccgct ttcactgctg caatggcgtt
    16621 acgctcaatg cacggtacct gaacctgccc cgcgaccggg tcacaagtca gaccgaggtt
    16681 gtgctccatc gcgatttccg ctgcgatgca aacctgcgcc gggcttgcgc ctaacagttc
    16741 tgccagacca gctgccgcca tagagcacgc cacgccaact tcaccctggc aacccacttc
    16801 ggcaccagaa atcgacgcgt tcatcttata aagagaacca atggcgctgg ctaccagcag
    16861 gtaacgagcc agtgagttag cgttcacttc gcggataaac ttatcgtagt acgccagcac
    16921 agccgggata atcccgcacg caccgttagt cggcgcagtc accacgcgac cgccagcagc
    16981 gttctcttcg ttcactgcca gtgcaaacat gttgatccag tcaacaaccg ccatcgggtc
    17041 agtggtggtt ttatcctggc tgaccagcat ccggcgtagt gccgcagcac ggcgtggaac
    17101 gcgcagtttg ccaggcaaca cgccttcggt ggaaataccg cgctcaatac cgccacgcat
    17161 gacttcccag acgttcgcca ggtgctgttc cagctcttct ttactgtgca gcgccagctc
    17221 gtttttcatc atcaggccag agagtgacag cccggtttcc tgacaatgtt tttgcagatc
    17281 ggctgctgaa ctgtacggat aaggaacttc aaccggtgcg ctatcctgct ggccaaaatg
    17341 ctcttcatca acgataaagc caccgccaat agagtagtaa gtctggctgt aaacgacttt
    17401 atcgcccgcc agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt tgtctgcatg
    17461 gaagttcatg cactgatcaa ccgggaactc cacttcatgc tgaccgtttg ccagcatcag
    17521 gcgaccatga gtattcacat cctgaataaa accggggatg gaatcgatat ccacggtatc
    17581 cggcaggtta cccgctaggc ccataataat ggcgatatct gtgtggtggc ctttaccagt
    17641 cagagagagc gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa gcaggttacg
    17701 ggcaatcaga tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg tatgagaact
    17761 ggaagggcca atgccgattt tgaaaatatc gaatacgcta atcataggaa atacatcgcg
    17821 ttaaaacgga ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga acagagagta
    17881 gaagattgcg gagattgcaa tcagacccat cacgacaacg aatacgttgc tgatgtgacc
    17941 gctgtactta cgcattgccg gtactttctg aattgcgtac atcggcatca ggaacaggat
    18001 catcgcgatg attgggccgc ccagggtttc aatcataccc aggatgctcg ggttcagggt
    18061 ggcaacaatc caggtcgtta ccagcatgaa cagcgcagtg atacggttca gcttgttgat
    18121 ttcgatagac ttacctttac cacgcagaga tttaatcacc ataccgttga agccttcacg
    18181 tgcgcccagg tagtggccga ggaaggattt ggtgatagcg ataatcgcga taatcggagc
    18241 catccacgcg ataaccggtg cgttaaagtg gttagccagg taagacagaa tcgagatgtt
    18301 ctgctcttta gccgcagcca ggtctgccgg agtcaggctc agtacgcagc tgaagacgaa
    18361 gaacattacg gtcagcacca tcatgatgtg tgcgaatgcc aggatcttcg agcatttctg
    18421 ttctgccata tcgccgtact cttcacgctt cgcaacggcg aaagaagaga tgatcggaga
    18481 gtggttgaac gagaacacca ttaccggaat tgccagccac agggtcatcc acagaccgtt
    18541 tccggttgca gatgcagtgt ccagagacag cgtttccagt gctgcgccgt tccactgcgg
    18601 gatcaggtac agagccagca gcatcagtac gccaacaaac gggaatacca gaatactcat
    18661 cgctttaacg atcatctgct caccaaagcg aacgatggtc atcataccca cgatcaggat
    18721 cagcgacaga atcgcacgcg gcggtggcgt catacccagc tggtgagaca tgaagctttc
    18781 aacggtattg gtgattgcca cgctataaac cagcaggatc gggtagatag cgaagaagta
    18841 gagcagggta atcagtttac ctgcgccaat accaaagtgt tcttctacaa cctcggtgat
    18901 gtcttcgccc gggtttttac cagacagtac gaagcgagtc aggccgcggt gagcaaaaaa
    18961 cgtcatcggg aacgcaagga tagccatgat gatcagcggg atcataccgc caacaccggc
    19021 gttgattggc aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc ccagcatcca
    19081 catggtgtct gtcttgcgcc aggcactacg agagtctttc gacgcaatcg tgctggtttg
    19141 agtcgtttcc atctatttct cctggaggaa tgcaaataat tcagctttta gccagttcag
    19201 cccgggaagg tgcctgaccg taaaaatgaa atctgtctat cggcgatggc ttaatcattt
    19261 accgccgtaa taatttgagg gcggaaagat acacacaagt tatgtatcta tcagtgatct
    19321 tgatcccaaa tacaataatc tacttggaag atagattatc ccaacgcttt actctgtaaa
    19381 tcaaaagtaa aagagaattt aagggcaata tttgcccatt aaacgacatc tgacttcttc
    19441 cggggcgcgc aatatatgta ggttgaactg aaaaaatcaa tacttttagt taatcatggc
    19501 gataatttct ttttcatagc gataaaatac ggtttatgaa aatagggaaa cgtttgcgtt
    19561 gcaacataaa ttgtatgaca tattaactgt gatgatattt gtcgcgtgag ggtggtaaga
    19621 aacattcata taacaaatga aatgagttga tgagtaagtg accaaaaacg agaaaacccg
    19681 gcaacaaacg tcaccgg
//
LOCUS       DADLSB010000070        19371 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.070, whole genome shotgun sequence.
ACCESSION   DADLSB010000070 DADLSB010000000
VERSION     DADLSB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.070"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(34..1863)
                     /gene="glmS"
                     /locus_tag="JN331_002341"
     CDS             complement(34..1863)
                     /gene="glmS"
                     /locus_tag="JN331_002341"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY7260471.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2025..3395)
                     /gene="glmU"
                     /locus_tag="JN331_002342"
     CDS             complement(2025..3395)
                     /gene="glmU"
                     /locus_tag="JN331_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY7260472.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3747..4166)
                     /gene="atpC"
                     /locus_tag="JN331_002343"
     CDS             complement(3747..4166)
                     /gene="atpC"
                     /locus_tag="JN331_002343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY7260473.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4187..5569)
                     /gene="atpD"
                     /locus_tag="JN331_002344"
     CDS             complement(4187..5569)
                     /gene="atpD"
                     /locus_tag="JN331_002344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY7260474.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5596..6459)
                     /gene="atpG"
                     /locus_tag="JN331_002345"
     CDS             complement(5596..6459)
                     /gene="atpG"
                     /locus_tag="JN331_002345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY7260475.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6510..8051)
                     /gene="atpA"
                     /locus_tag="JN331_002346"
     CDS             complement(6510..8051)
                     /gene="atpA"
                     /locus_tag="JN331_002346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY7260476.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8064..8597)
                     /gene="atpH"
                     /locus_tag="JN331_002347"
     CDS             complement(8064..8597)
                     /gene="atpH"
                     /locus_tag="JN331_002347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY7260477.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8612..9082)
                     /gene="atpF"
                     /locus_tag="JN331_002348"
     CDS             complement(8612..9082)
                     /gene="atpF"
                     /locus_tag="JN331_002348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY7260478.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9144..9383)
                     /gene="atpE"
                     /locus_tag="JN331_002349"
     CDS             complement(9144..9383)
                     /gene="atpE"
                     /locus_tag="JN331_002349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY7260479.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9430..10245)
                     /gene="atpB"
                     /locus_tag="JN331_002350"
     CDS             complement(9430..10245)
                     /gene="atpB"
                     /locus_tag="JN331_002350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY7260480.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10254..10634)
                     /gene="atpI"
                     /locus_tag="JN331_002351"
     CDS             complement(10254..10634)
                     /gene="atpI"
                     /locus_tag="JN331_002351"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY7260481.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11251..11874)
                     /gene="rsmG"
                     /locus_tag="JN331_002352"
     CDS             complement(11251..11874)
                     /gene="rsmG"
                     /locus_tag="JN331_002352"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY7260482.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11938..13827)
                     /gene="mnmG"
                     /locus_tag="JN331_002353"
     CDS             complement(11938..13827)
                     /gene="mnmG"
                     /locus_tag="JN331_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY7260483.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14206..14649)
                     /gene="mioC"
                     /locus_tag="JN331_002354"
     CDS             complement(14206..14649)
                     /gene="mioC"
                     /locus_tag="JN331_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY7260484.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14739..15197)
                     /gene="asnC"
                     /locus_tag="JN331_002355"
     CDS             complement(14739..15197)
                     /gene="asnC"
                     /locus_tag="JN331_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY7260485.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15349..16341
                     /gene="asnA"
                     /locus_tag="JN331_002356"
     CDS             15349..16341
                     /gene="asnA"
                     /locus_tag="JN331_002356"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY7260486.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16346..17797)
                     /gene="viaA"
                     /locus_tag="JN331_002357"
     CDS             complement(16346..17797)
                     /gene="viaA"
                     /locus_tag="JN331_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAY7260487.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17791..19287)
                     /gene="ravA"
                     /locus_tag="JN331_002358"
     CDS             complement(17791..19287)
                     /gene="ravA"
                     /locus_tag="JN331_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY7260488.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELQEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4619 a         5127 c         4859 g         4766 t
ORIGIN      
        1 cggtcggcag ccctttttta tctatgatag tgattactca accgtaaccg attttgccag
       61 gttacgcggc tggtcaacgt cggtgccttt gatcagcgcg acatggtaag ccagcagctg
      121 cagcggaacg gtgtagaaga tcggtgcaat cacctcttcc acatgcggca tctcgatgat
      181 gtgcatgtta tcgctactta caaaacccgc atcctgatcg gcgaagacat acaattgacc
      241 gccacgcgcg cgaacttctt caatgttgga tttcagtttt tccaacagtt cgttgttcgg
      301 tgcaacgacg ataaccggca tatccgcgtc aatcagcgcc agcgggccgt gtttcagctc
      361 gcctgcagcg taggcttcag cgtgaatgta agagatctct ttcagcttca atgcgccttc
      421 cagcgcgatt gggtactgat cgccacggcc caggaacagc gcgtgatgtt tgtcagagaa
      481 atcttctgcc agagcttcaa tgcgtttatc ctgagacagc atctgctcaa tacggctcgg
      541 cagcgcctgc agaccatgca caatgtcatg ttcaatggag gcatccagac ctttcaggcg
      601 agacagcttc gccaccagca tcaacagcac agttaactga gtggtgaatg ctttagtgga
      661 cgccacgccg atttccgtac ccgcgttggt catcagcgcc agatcggatt cgcgcaccag
      721 agaagaaccc ggaacgttac agattgccag cgaaccaagg taacccagct ctttcgacag
      781 acgcaggccc gccagggtat ccgcggtttc gccagactgt gacaaggtga tcatcaggct
      841 gttacgacgc acggcagatt tacgatagcg gaattcagag gcgatttcga cgtcgcacgg
      901 aatacctgcc agcgattcaa accagtagcg ggaaaccata ccggagttat aagaagtacc
      961 acaggcgagg atctgaatat gctcaacctt cgacagcagt tcgtcggcgt tcggtcccag
     1021 ctcgcttaaa tcaacctgac cgtggctgat gcgcccggta agggtgtttt ttatcgcgtt
     1081 cggctgttcg tagatctctt tctgcatgta gtgacggtaa atacctttat cgcccgcatc
     1141 atattgcaga ttggattcga tatcctgacg ttttacttcc gcgccagttt tatcgaagat
     1201 gtttaccgaa cggcgagtga tttccgcaat atcgccctct tcaaggaaga taaagcgacg
     1261 ggtcaccggc aacagcgcca gctggtcaga agcgataaag ttttcgccca tacccaggcc
     1321 aatcaccagc ggactaccag aacgtgccgc cagcagggta tccgggtgac gggagtccat
     1381 gatcactgta ccgtacgcac cgcgcagctg cgggatagca cgcagaacag cctcacgcag
     1441 agtcccgcct tgtttcagct cccagttcac cagatgggca atcacttcgg tgtcggtttc
     1501 agaaacgaag gtatagccac gcgcttttaa ctcttcacgc agcggttcat ggttttcgat
     1561 gatgccatta tgcaccacca caatgtgttc agaaacatgc ggatgcgcgt tagcttctga
     1621 aggttcaccg tgggtcgccc agcgagtgtg agcaataccg gtgccgccat gcagaggatg
     1681 ttcttccgct gcctgagcca gcatctggac tttaccgagg cgacgcaggc gagtcatatg
     1741 accttccgca tcaacaacgg ccagaccggc agagtcatat ccgcggtatt ccagacgacg
     1801 taaaccttca agaaggattt ctgctacatc acgttgcgcg atcgcgccaa caattccaca
     1861 catagttttt gattccgatt tatatcgttg tcggtcaacc tgtatgcccg ttttttcggg
     1921 cgccccgagc cttgtagaga gtggggttat ttttatagtt actgcttgtg ggagggagat
     1981 tatgttatct cctcatccca gatgaccgga ttacccggcc agaattactt tttctttacc
     2041 ggacgacgcc agccttcttt ctgagtctgc ggcacacggc tgatagctaa tgcattttcg
     2101 ccgacattac gcgtcacagt tgtacccgca gcaatggtcg cgcctttgcc tactgttacc
     2161 ggggccacca gctgagtgtc ggaaccgaca aacacatcgt cgccgataat ggtcttaaat
     2221 ttattcgcac catcgtagtt gcaggtaatg gttcccgcgc cgatgttaac gttatcgcca
     2281 atttccgcat cgcccaggta agtcagatgc ccggctttcg tgcctttacc cagacgcgct
     2341 tttttcatct caacgaagtt accaacgtgt gcaccttcca gcaactcagc accaggacgc
     2401 agacgggcaa acgggccaat agtacaggcc gctgccagat tcgcgtcttc cacaacggta
     2461 tacggactga tttcgcaatc atcgccaatc acgctgtttt taatcacgca accggtgccg
     2521 attttcacgc gatgaccgag agtcacgttg ccctcgatga taacgttagt atcaatttca
     2581 acatcgcgcc cgtgagtaag cgtaccgcgc agatcaaaac gcgccggatc gcgcagcata
     2641 acgcctgcta acagcagttt ttcagcctgt tcggactggt aaacgcgctc cagacgggag
     2701 agttgcaggc ggttattcac accttctact tcgcttaaac gttgcggatg aacggcgacg
     2761 atttcacgcc cttcctgata cgccagcgca ataatgtcgg tgatgtagta ttcgccctga
     2821 gcattattgt tggtcagctt cgccagccag cgtttcatat ctgcgccgtt ggcaatcaga
     2881 atgccggtgt tgatctcctg aatctgacgc tgctcgtcgg tggcatcttt gtgctcaaca
     2941 atgccggtaa ctttgccatt ttcacgggtg atacgtccat aaccggtcgg atcatccagt
     3001 ttcaccgtca gcagaccaat gccaccctgg ggtttagcat cacgcaggcg ctggagtgtt
     3061 tcgacagaga tcagcggcac gtcgccgtag agcattaaaa tgtcttcatc atcggcaaag
     3121 aaaggtgcgg cctgctgcat tgcatgaccc gtacccagct gctctgcctg aagcacccag
     3181 ttaaggttgt cgtctttcag cgcctgttta agcagatcgc cgccgtgacc gtacaccagg
     3241 tgaacgtgcg ctgcgcctaa ttcattcgca gcatcaatga catgctgaac catcgctttc
     3301 ccggcaaggg tatgcagcac tttcggaaga tcggaataca tgcgcgtgcc tttgcctgcg
     3361 gcaaggatca ctacgctcat agcattattc aacatacgcg tcctgactgt aatttgagaa
     3421 cgaatttaaa ccgcttcacc ttgaaaaaac tacatttttt tcatcgtgaa atggacagag
     3481 gataaattgt tcaataacgg attatccccg ctgacgaaag tgccattttc gaccatcgtc
     3541 acgccttttg tctgcagaaa attaaggcag taccttcatt taagcgtcag gtggatgttt
     3601 ttgctcttat ttcgatcaat gaataaacgg gaaaacaggg gtgttttata tcagcaggat
     3661 ctatgtgaac gctcttcagg acggttcaac gcgtaaaaaa aagccagcct gtttccagac
     3721 tggcttttgt gcttttcaag ccggtgttac atcgcttttt tggtcaactc gataacgcgc
     3781 agctgcgcga tcgctttggc cagttccgca gacgcctgag cgtaatctac gtcgccgtga
     3841 gagctgctaa tgtgctcttc agccttacgt ttcgcttcca tggctcgcgc ttcgtcgaga
     3901 tcctggccgc gaattgcggt gtcggccaga acggtcacgt tgccaggctg cacttcaaga
     3961 atgccgccag acagatagat aaactcttcg tgaccgtgct gtttcacgat gcgaatcata
     4021 ccaggcttaa tggcggtgag cagcggtgcg tggccagggt agatccccag ttcaccttcg
     4081 ctacccgtta cctggatttt ctcgaccaga ccagagaaca tttgttgctc tgcgctgacg
     4141 acgtccaggt ggtaagtcat tgccatatca ccctccgatt aaggcgttaa agttttttgg
     4201 ctttttccac agcttcttcg atggaaccga ccatgtagaa cgcctgctcc ggcaggtgat
     4261 cgtattcgcc ttccatgatg cctttaaagc cacggatggt gtctttcagg gagacgtatt
     4321 tacccggaga accggtgaat acttctgcca cgaagaacgg ctgggacagg aagcgctgga
     4381 tcttacgagc acgcgctacc accagtttgt cttcttcaga cagttcatcc atacccagga
     4441 tggcgatgat gtctttcagt tcctgataac gttgcaggat ggactgaacg ccacgcgcgg
     4501 tgtcgtagtg ttcctgacca accaccagcg ggtccagctg acggctggtg gagtccagcg
     4561 ggtcaacggc cgggtagata cccagagacg cgatctgacg gctcagtacc acggttgcgt
     4621 caaggtgcgc aaaggtggtt gccggagacg ggtcagtcaa gtcatccgca ggtacgtata
     4681 ccgcctgtac ggaggtgata gaaccagttt tggtggaggt gatacgttcc tgcagaacgc
     4741 ccatctcttc cgccagggtc ggctgataac ctaccgctga aggcatacgg cccagcagtg
     4801 cggatacttc cgtaccggcc agggtgtaac gatagatgtt gtcaacgaac agcagaacgt
     4861 cacgaccttc gtcacggaat ttctcagcca tggtcagacc ggtcagagca acgcgcagac
     4921 ggtttcccgg cggctcgttc atctggccat acaccaggga tactttgtcg ataacgttgg
     4981 agtcggtcat ttcgtggtag aagtcgttac cctcacgagt acgttcacct acgcccgcaa
     5041 acacagagta accggagtgc tcgatcgcga tgttacgaat gagctccatc atgtttacgg
     5101 ttttacctac acccgcacca ccgaacagac caactttacc gcccttagcg aacggacaca
     5161 tcaggtcgat aactttgata ccggtttcca gcagttcctg agagtttgac agctcttcgt
     5221 aggaaggtgc tgcgcggtga atcgcccaac gctcttcttc accgatctcg cctttcatgt
     5281 cgaccggttc acccagtacg ttcatgatac ggcccagagt cgctttacct accgggactt
     5341 cgatcgggtg ttcgaggtct tttacatcca gaccgcgacg cagaccgtcg gaggaaccca
     5401 ttgcgatggt acgcacgata ccgccgccga gctgctgctg aacttccagc accagacgct
     5461 cattaccatt ttgcacctca agagcatcgt acacgcgcgg tacggcatcc tgagggaatt
     5521 cgacgtcaac tacggcgccg attacctgga caatctttcc agtagccatc ttaaatcctc
     5581 tacgaaataa cctgtttaaa ccgcggcggc ccccgagacg atctcggtga gttcctgagt
     5641 aatgctggcc tgacgagctt tgttgtatac caactgcagc tctttaatca ggctgccgcc
     5701 attgtcggtc gcggctttca tcgccaccat acgggcggcc tgctcgctgg ccaggttttc
     5761 aaccacgccc tgataaacct gagattcgac ataacgacgc agcagggtat ccagcaacgc
     5821 cttcggatcg ggttcgtaca ggtaatccca ggatttatgt ttcagatcat catcatctga
     5881 tgccggtaac ggcagcagct ggctgatggt cggaacctga gacatggtgt taataaattt
     5941 gttgctgaca atgtaaagct tgtccagacg gccttcgtcg taggcctgca acatcacttt
     6001 taccggaccg atcagttcgg acagggaagg gttatccccc atgccggtga cctgggcaac
     6061 aacattgccg cccacggagt tgaagaacga cacgcctttc gagccgatca ttgcgaggtc
     6121 gcattgaacg cctttgtcgg tccaggtctt catttccgcc agcagttttt tgaacaggtt
     6181 aatgttcaaa ccaccgcaca aaccacggtc ggtcgacacc accaggtagc ccacgcgttt
     6241 aacgtcgcgg tcttccaggt aagggtgctt atattccaga ttaccgtgtg caaggtgacc
     6301 aatcactttg cgcatggttt ctgcataagg acggctggcc gccatgcgat cctgcgattt
     6361 acgcattttg gaagcggcga ccatctccat cgctttagtg atcttttgcg tgttctggac
     6421 gcttgcgatc ttactacgta tctcttttgc gccggccatg agcttctcct caatgccttg
     6481 cggcctgccc taaggcaagc cgccagacgt taccaggatt gggttgcttt gaaggaatcg
     6541 aggatgcctt tcagcttgcc ttcgatttcg tcgttgtagc caccggtctg gttgatctct
     6601 tgcatcaacg gagcgtgatc acggtcgacg taagccagca gagcggcttc gaagctgcca
     6661 attttcgaca gttcaacatc cgccaggtaa ccacgttctg ctgcgaacag aaccagagac
     6721 tgctgcgcaa cggacatcgg cgcatactgt ttctgtttca gcagttcggt cactttctga
     6781 ccgtggtcaa gctgcttacg tgttgcatcg tcaaggtcgg atgcaaactg agagaacgct
     6841 gccagttcac gatactgtgc cagagcggta cggataccac cggacagttt tttcatgatc
     6901 ttggtctgtg ctgcaccacc aacacgggat acggaaatac ccgggttaac cgcaggacga
     6961 ataccggcgt tgaacaggtt ggtttccagg aagatctgac catcggtaat ggagattacg
     7021 ttggtcggaa cgaacgcaga aacgtcaccc gcctgagttt cgataatcgg cagtgcggtc
     7081 agagaaccgg ttttcccttt cacttcacct ttggtgaagg cttcaacgta ttcggcgtta
     7141 acacgtgcag cacgctccag cagacgagag tggaggtaga aaacgtcgcc cgggaatgct
     7201 tcacgtcctg gcggacgacg gagcagcagg gagatctgac ggtaagcaac agcctgttta
     7261 gacaggtcat cgtaaatgat cagcgcatct tcaccgcggt cacggaagta ttcgcccatt
     7321 gcgcaaccgg catacggtgc caggtattgc agtgcagcgg attcagacgc ggttgctacc
     7381 acaacgatgg tgttagccag tgcgccgtgc tcttccagtt tacgtaccac gttagaaatg
     7441 gtggacgctt tctggccgat agcgacatag atacatttga taccggaatc gcgctggttg
     7501 atgatggcat cgatagccag tgcggtttta cctgtctgac ggtcaccgat gatcaattca
     7561 cgctgaccac gaccgattgg gatcatggag tcaacggctt tataaccggt ctgtaccggc
     7621 tgatctacgg actgacgttc gataacgccc ggagcgattg cttctacagc agagaagccg
     7681 tcgtgatcca gcggaccttt accgtcgatt ggtgcaccca gagtgttaac cacacggccc
     7741 agcaggccac ggccaaccgg aacttccagg atacggccag tacacttaac tttcatgcct
     7801 tcggcaaggt cagcgtacgg acccataaca accgcaccta cagagtcgcg ctcgaggttc
     7861 agtgcgatag cgtaacggtt acccggcagg gagatcattt caccctgcat acaatcggcc
     7921 aggccgtgaa tgcggataac accgtcactt acagaaacaa tagtaccttc gttgtgagct
     7981 tcactcacaa cattgaactg agcaatgcgc tgcttgatca gttcgctgat ttcggtggaa
     8041 ttcagttgca tgctccagtc cccttaagac tgcaagacgt ctgcaaggcg ctcaagacga
     8101 ccgcgtacgc tgccatcaat gaccatatca cccgctcgga tgataacgcc tgccattaca
     8161 gacttatcga ttttgcaatt cagcttaact ttgcgtgaca gacgtttttc catcgcagca
     8221 gaaattttcg cgagctgttg ttcactcagt gcggcagcgg aaatgacgtc tacctcagcg
     8281 gtagcctcac tcacggcacg aaggtgaata aactgctcca gaacatccgg gagcgcgtta
     8341 agacgaccat tttcagccat tacccgaatc aggttctgac cgttttcgtc cagttgctca
     8401 ccacaaactg cgataaacga ctcggcgagc gtttctggcg caagcgcgcc agagagaagc
     8461 tctgccattt gttcgttttt ggttacctcg gcggcaaacg ccagcatgtc ctgccagcgt
     8521 tctacacttt ggtgttcgac ggcaaagtca aaagctgctt tggcgtaggg gcgagctacc
     8581 gtaataaatt cagacatcag cccctccctc cttacagttc agcgacaagt ttatccacga
     8641 tgtcgctgtt agcagcttca tccacggaac gttcgatgat cttctcggcg ccagcaacag
     8701 ccaggatagc aacttgctta cgcagctctt cacgggcacg tttacgctcg gcttcaattt
     8761 ctgcctgcgc ctgggccacg attttagtac gttcctgttc tgcctcagct ttcgcttcgt
     8821 ccagaatctg cgagcggcgt ttgttcgcct gctcgatgat tacctgggct tccgctttcg
     8881 cttttttcag ctggtcggtc gcgctggcct ttgcaaggtc aaggtcctta tgtgctcgtt
     8941 ctgcggaagc aaggccgtca gcaatttctt tttgacgttt ttcgatggct gccattaatg
     9001 gcggccatac gtacttcatg cagaacagaa cgaacaggac aaacgcgatg gcctggccga
     9061 ggattgttgc gttaagattc acagcacaat gcctctattt agttaacgtt ctgatattgc
     9121 tctttaaata aaagcaacgc ttactacgcg acagcgaaca tcacgtacag acccagacct
     9181 acagcgatca tcgggatagc atccaccaga cccataacga taaagaactg agtacgcagc
     9241 agaggaatca gatcaggttg acgcgctgcg ccttccaaga atttaccccc gaggatgccg
     9301 ataccgatcg cagcaccgat tgccgccaga cccatcatca cagcggcagc catgtacagc
     9361 agatccatat tcaggttttc catgacagtc tccagtttgt ttcagttaaa acgtagtagt
     9421 gttggtaaat taatgttctt cagacgccat cgacagatag acgatcgtca gaaccatgaa
     9481 gatgaaggct tgcagcgtaa tgatcaggat gtggaaaatg gcccacggca cattcaggat
     9541 ccactgtgac caccacggca acagaccagc aatcagaatg aaaatcagct caccggcata
     9601 catgttaccg aacagtcgca aaccgagtga aactggtttg gacagcaggc ttaccccttc
     9661 aaggattaag ttgacaggaa tgaacgccca gtgattgaac ggctgcagcg tcaactcttt
     9721 cgtgaagccg ccgatgcctt tcattttgat gttgtagaac agaatcagga taaatacgcc
     9781 cagtgccata gacagcgtta cgttcacgtc cgcagacgga accacacgca gtgcaggcag
     9841 acccagtaca tgttcagcaa tgtacggcag caggtcgata ggcagtaaat ccatcaggtt
     9901 catcaggaat acccagacga agatcgtcag ggccagcgga gcaatcagct tgcttttgcc
     9961 atggtacatg tctttcacgc taccattaac aaagccgatc accagctcaa tcgcggtctg
    10021 aaacttacct ggcacaccgc tggtcgcctt tttggctacg ctacggaata aaaccaggaa
    10081 caacagaccc agcaccaccg agaagaacat ggagtcaata ttgattgtcc agaaggtggc
    10141 tggggggttt tgtggatcca ccagcgagaa tgtacgcagg tccagctgaa ggttattcag
    10201 gtggtgtcct atgtaatcct gcggcgtcat attttctgaa gccatgatgc cttttaccct
    10261 ttgttgttaa ttacagccgg tgccagtatc tgaaccacca gcaccaaaac ccacgtaacg
    10321 atcagcggca agaataccgc ctttaaaacc gccaacgcca ccaccagtaa caccaacatc
    10381 gccagaactt tgaaagcttc gccaaatgcg aatgtccaag ccacccggcc tttcgctggt
    10441 gtatgcgcct ggtgacgcca ggcaaatatc ataaacaaaa cgttaggcag aaagactgcc
    10501 aggcccccgc ttattgcaga gacgccccag aaggggtctt tgaggctgaa cagcaatcca
    10561 cttgctatca ccaccagtaa ctgaacgagc agaagcttcc gagcaacgtt tcgactcacg
    10621 agcgacacag acatcacgtt tttcactcct gctcccttcg aggtatgccg cgtgtcgtat
    10681 aaaactttct ttaaggctta gagtcaagca tcaaaaagcg gtcaaattat acggtgcgcc
    10741 cccgtgattt caaacaataa gtagccaaaa ggtgaataaa tgtttaaata tttttccagt
    10801 gcatacaatt gcgacttttc cgctaaccct gttcgatcat gaaaaactgt aaataacgcg
    10861 taaacactgg tgataaagcg tgcttcagat cacatattgc gcatgttcgc gcacagcata
    10921 tttatttact tggcaaatga tgcctttgca agtttatgat atttcagtct aaaaacagat
    10981 actgttttaa taaatgacat ttacacaaca aaaaccaccc attgacattt ttaataatgt
    11041 tttaacagcc aatgatggtt cttagcgccg atttttagca gactgatatt ttcactaatg
    11101 acttattttc tgcttaccaa aaaaagccac gttatcttgt tgatgcaaaa gagtgaacgt
    11161 ggcgttaaat gtaaccagtt atatcagtag aaaacctggt tgttgttaac agtctaaccg
    11221 gtcaattttt tatgattttt ttgataaaaa ttaaatttta tttgctttaa tcaccaccag
    11281 atgacgttcg ccatccaggg ctggaacctg aagtttaacc actgattcga cctgatattc
    11341 ttcgggcaac aaagcgattt catcttccgg catttgccct ttcagcgcgt agaaacggcc
    11401 ttgctcacca ggaagatggt ggcaccagct caccatatcg ttcagagagg caaaagcgcg
    11461 gctaattacg ccatcaaatg gcggctctga aggaaactct tctaccctgc tctgtactgg
    11521 ttcaatattc tccagtttaa gctcatgttg cacctgacga aggaaacgca cgcgtttacc
    11581 aaggctatcc aacagagtga aatgggcttc aggacgcacg atagagagtg gaatgcctgg
    11641 cagtcctggt ccggtgccga catcgataaa ccgttcacct tgcagatacg gtgccaccac
    11701 aatgctatcg agaatatggc gtaccagcat ctcattagga tcgcggaccg aagtcaggtt
    11761 gtacgctttg ttccatttat gcagcatatt cacgtaggca ataagctggt ttttctggtg
    11821 atcagtaagc gaaatacctg cgtctttcag cagtaaggag agtttgttga gcacggtgat
    11881 tacctgttct tgatgcgttg cctggtaagc gggtgcttac caggcatttt taatgcgtta
    11941 tgcgctacga cgcagcatac cctgtttttt cagccacacc agcagaatgg agatggccgc
    12001 aggcgtgacg ccagaaatac gcgaagcctg gccgatagag gccggtttgt gatcgttaag
    12061 tttggcgatc acttcgttag aaagaccgga tacctggcgg taatccagtg tcgcgggtag
    12121 cagggtgttc tcgttacgca gctgcttttc gatctcatct tgctggcgcg cgatataacc
    12181 ttcgtattta acctgaatct caacctgttc cgccgcctgt tcgtctgtca acgcaggggc
    12241 aaacggcgtc agcgtggtta atttttcata agtcatttcc ggacgacgca gcagatcttc
    12301 accactggct tcacgggaaa gcggcgcagt caggtgagca ttcacttcgg ctgcagcttc
    12361 cgccgacggg gttacccagg tcgatttcag acgctgacgc tcacgctcga tattctcaag
    12421 tttctcgtta aagcgcgccc aacgttcgtc atccaccagg cccagttcac gaccgatttc
    12481 agtcaaacgc agatccgcat tatcttcgcg cagcatcaga cgatattctg cgcgcgaagt
    12541 aaacatacga tacggttctt tggttcctaa agtgcacagg tcatcaacca gtacgccgag
    12601 atacgcctga gaacgtgccg gagcccaacc ttctttgtca gcagacagac gggcagcgtt
    12661 aagaccggcc agcaaacctt gcgcagcggc ttcttcgtaa ccggtagtgc cgttaatctg
    12721 accagcaaag aacagcccct ggataaactt gctctccagc gtcggtttca ggtcgcgagg
    12781 atcgaagaag tcatactcaa tggcataacc cggacgcacg atcttcgcgt tttccatccc
    12841 ctgcatagag cggacgattt gcatctgcac atcgaacggc aggctggtgg agataccgtt
    12901 cggataaatt tcattagagg tcagtccttc cggttcaagg aagatctgat gctgatttct
    12961 gtctgcgaag cgcatgactt tgtcttcgat cgacgggcag tagcgtgggc cgacaccttc
    13021 gatcacccct gcatacattg ggctacgatc gaggttactg cggatcacat catgggtttt
    13081 ctcgttggta tgagtgatat aacacggcac ctgctgggga tgctgggacg cattgcccat
    13141 aaacgagaat accggcattg ggttatcgcc atgctgttgc gccagcacgc taaagtcgat
    13201 ggttcgagca tcaatacgcg gcggagtacc ggttttcaga cgaccaacgc gcagcggcag
    13261 ttcacgcaaa cggcgagaga gcggaatgga tggcggatcg ccagcacggc caccgctgta
    13321 attatccaga ccgatatgaa ttttgccgtc gaggaacgtc ccaacggtga gcaccacagc
    13381 tttggcacgg aacttcagtc ccatttgggt aacagcaccg accacgcgat cgttttcgac
    13441 aataagatct tcaaccgcct gctggaagat catcaggttc ggttggttct ccagcgccgt
    13501 acgtaccgcc tgacggtagc gcacacgatc cgcctgagct cgggtagcgc gaaccgccgg
    13561 tcctttgctt gcgtttagta tcctaaactg gatacccgct tgatcgatcg ctttcgccat
    13621 cagaccgccg agtgcatcca cttcttttac cagatgtccc ttcccaatac cgccgatcgc
    13681 cgggttgcag ctcatctgcc ccagagtgtc gatattgtgt gtcaaaagca gagtctgttg
    13741 acccatacgc gccgcagcca tcgcggcctc ggtgcctgca tgacccccgc caatgatgat
    13801 gacgtcaaaa ggatccggat aaaacatggt gattgcctcg cataacgcgg tatgaaaatg
    13861 gattgaagcc cgggccgtgg attctactca actttgtcgg cttgagaaag acctgggatc
    13921 ctgggtatta aaaagaagat ctatttattt agagatctgt tctattgtga tctcttatta
    13981 ggatcgcact gccctgtgga taacaaggat ccgactttta agatcaacaa cctggaaagg
    14041 atcattaact gtgaatgatc ggtgatcctg gaccgtataa gctgggatca gaatgagggg
    14101 ttatacacaa ctcaaaaact gaacagcggt tgttctttgg ataactaccg gttgatccaa
    14161 gcttcctgac agagttatcc acagtagatc gcacgatctg tatacttatt tgagtaaatt
    14221 aatccacgat cccagccatt cttctgccgg atcttccgga atgtcgtgat caagaatgtt
    14281 gatcttcagt gtttcgcctg tctgttttgc accggaattt ttgagttctg cctcgagttt
    14341 atcgatagcc ccacaaaagg tgtcatattc acgactgcca ataccgattg cgccaaagcg
    14401 gaccgcagaa agatcgggct tctgttcctg caatgcttca tagaaaggag aaaggttgtc
    14461 cggaatatct ccggcaccgt gggtggagct gataaccagc cagatccccg aggcagataa
    14521 atcttccaac agcggaccgt gcagcgtttc ggtggtaaaa cccgcctctt ccagcttttc
    14581 agccaggtgt tctgctacat attcggcacc gccgagggtg ctgccgctga taagagtgat
    14641 atctgccata aaccgccacc tttattaaga gtggcgtatt gtacgctgtg aacgcgttgg
    14701 gatctacctg tggaaaagta tgggattaaa aaagctgatc agggcttgat ggtacgcatg
    14761 atcgggtttt gcaggacgat caatgtctcg gtggactgaa tttcatcaat tgtttggatc
    14821 ttgttgataa gtacatgctg gagagcgtcg atcgaacggc acataacttt tataaagatg
    14881 ctgtagtggc cggttgtgta ataggcttca gtgacttcat caaggctttc cagctttgcc
    14941 agcgcggaag ggtagtcttt ggcgctcttt aatataatgc cgataaagca gcctacgtca
    15001 taaccgagct gcttcgggct gacatcaata cgcgccccgg taatgatccc cgcctgcttc
    15061 attttctcta ctcgaacgtg aatcgtcccc ggactgacgc caaattgttt cgccagttcg
    15121 gcgtaagcgg tgcgcgcatt gcccattaat gcttccagga tgccacggtc cagattgtcg
    15181 atcagataat tttccatagg attttcttat gcagattgat gattcattct attttagcct
    15241 tctttcttaa tgaatcaaaa gtgtgttagg ctttttattg aatgattatt gcatgtgtgt
    15301 cggtttttgt tgcttaatca taagcaacag gacgcaggag tataaaaaat gaaaaccgct
    15361 tacattgcca aacaacgcca aattagcttc gtgaaatctc acttttctcg ccaactggaa
    15421 gaacgtctgg ggctgatcga agtccaggcg ccgattctta gccgtgtggg ggatggcacg
    15481 caggataact tgtcgggctg tgaaaaagcg gtgcaggtaa aagtgaaagc tctgcctgat
    15541 gcccagttcg aagtggttca ttcactggcg aagtggaaac gtcagacctt agggcaacac
    15601 gacttcagcg cgggcgaagg gctgtacacg cacatgaaag cccttcgccc cgatgaagac
    15661 cgtctttctc cgttgcactc ggtctatgtt gaccagtggg actgggaacg cgtaatgggc
    15721 gacggtgagc gtcaattctc gactctgaaa agcacggtag aggcgatctg ggcgggaatt
    15781 aaagcaaccg aagctgcggt taacgaagag tttggcctgg caccgttcct gccggatcag
    15841 attcacttcg tacacagcca ggagttactg tctcgttatc cggatctcga tgccaaaggg
    15901 cgtgagcggg cgattgcgaa agatcttggc gcggtattcc tcgtcgggat tggcggcaag
    15961 ttgagtgatg gtcatcgtca cgacgtgcgc gcaccggatt atgatgactg gagcaccccg
    16021 tcagagctgg gccatgcggg cctgaacggc gatattctgg tgtggaaccc ggtactggaa
    16081 gatgcgtttg agctttcttc catggggatc cgcgtggatg ccgacacgct gaagcatcag
    16141 ctggcgctga ccggtgatga agatcgtctg cagctggaat ggcatcaggc gctgctgcgc
    16201 ggtgaaatgc cgcagaccat cggcggcggt atcggccagt ctcgtttgac tatgctgctg
    16261 ctgcaactgc cgcatatcgg ccaggttcag tgtggagtat ggccagctgc tgttcgcgag
    16321 agcgtccctt ctctgctgta ataatttatc gccgccagcg tctgagcagg cggcttcgca
    16381 tcccggtatc aaagcgccag atatgatcga aaatgcgcat gatgccgggt ttgccgtgtg
    16441 ccgacatcgc cacggcatga aagcgatgct gatgtacccg ctgcagctct ttcactttac
    16501 tcgtcacgtc gtcaggcaac cgctgagcga taaaatcaga aatcaccacc gcatcggcat
    16561 caaaccattc cctgctttgc aagcgttcca taatggcgcg aaaacaactg gcaagatcgg
    16621 tgccgccacg aaactgctgg cttaaaaaac ggattgcttg ttcgatgcct tgtgggcctg
    16681 aaagctcata acggacgatc tcggtggaaa atagcataat ataacagcgc cggttttcag
    16741 cgagagcaat gcgcatcaaa gccaggcaga acgctttcgc acactgttca ttaaagccgc
    16801 ccattgagcc ggaagtatcc acacagacaa taaacggccc gcgcggctgt tcatcgtaat
    16861 ctttatgtac caccggacgt tcgagcactt tttcacgcca cgactcaccg tgcaggcgat
    16921 aggtgagcaa ctgtttttcc accagccgac ggtaaaactc atactccagt tccgttatcc
    16981 ctagtgtcgc cagttctggc ggcaggagac gtaaaatatc atcgctttgt tgcagaccat
    17041 caacctgctc aggaaccgtc gccggttcgc gcaccatggt gcggaaggtt tccatctgcg
    17101 catcgttgcg cggtattgat ttggcttccc gagaacgccc cagctgctct gctaggcgtt
    17161 tcagttccgg ctgttcgtta agaaattcac cgtatttcac aatcaactga tagtcgccac
    17221 gtttaagctg accggcgctc atatcccaca gacgaccagc tgcggtattg ttatctgcga
    17281 gaatcggttc aagttgtccg ctcagcgtca tgcgttcctg aacttcactt aacagttgtt
    17341 cgcgttcttc ttctaatagc tgttgattta acgtcgttgc ttgcacgatc agacttaaac
    17401 gccaacgctg gagaaaaagc gtgtgtaacg ctgaagttat tgtgctgtta gcatcaacca
    17461 actgccgggc ttgttctgcc catggggaat tcagacgatg cagtaagtcc aggatctgtg
    17521 gtagctgcac aataaactgc ggcgtggaga ggagctggct ttgctgatag cacatcacct
    17581 cttcggtgag ttccggcggg actcgggcat ctttcagccg actgcgcagc gcctcacgcc
    17641 agcggggaac atcatcagtg attgccgcct tcagtcgtgg gaatttttca aagaagaccg
    17701 ccagctgcgg tgaggccagc agtgcgatga tcatctcttc gatcaatccc tcttcgctga
    17761 cggccagcat cacattaagc gtatccagcg ttagcattgt tgtgcctggc gaatctgtgc
    17821 accgacatcc tgtaggctgg cttcaatgcg acctaaccag tcgccaggga taaacagaca
    17881 tttttgctgt tcgctgaata aagcgtgttg cttacgccag tcgctttcaa gctcttccag
    17941 ttgttgtttt atttcacctg gcagaccttc agccgatgaa ccgggaagtg ccagcgtact
    18001 gccttgtaaa cttacatcgc gtacaacaag atgttgggtg ctatcaactt ccagattcag
    18061 tttctgggca aagccgatac cgttcagttt gccgcgaatt tcaccacctt tactcagcca
    18121 ctgttccagc gcgctacgct caaaggagat atgaaccact tccatatcat gcagttttaa
    18181 cggtttttgc agcagcagag tcagagtgga agcagtaacg ttaacaggaa gttgatactg
    18241 ctgacgacgg ctgaaaatgc cgcccagacg aattaacgtt aaggctgttt tatcgctttg
    18301 ttgctgctgg agttgcaggt gacgttgcac aatcgcgccc aggcgggtca acatcccttg
    18361 ctgttgccag gcatgaccgg tcatcaatac atcaatttgt tgttgtatca aattcaggct
    18421 ttgcgcgtca taccacaggc aatctttcag caaaatgaga tcaaccgggg caacagcact
    18481 gcgaccgcta aaaaaggcgc tggcctgcaa taagcggatc gcttttttcc agcgacgatc
    18541 cgagacataa ggcgcatccg gtaatttatc cagttgctgg cgcagcataa aaatcagctc
    18601 aaatacatga tcgggcagcg taatttcacc aatctctttc tgccagcgtt catattcttc
    18661 atctgtgacc tgcaaggcat caggaaccgg attgtcgttt tcatcctgtt gactggtcag
    18721 catggagcgg aaattcgctt tatcttgcac tttatctaac catagacgaa tcagcatgcg
    18781 gtcatataac gcttccagac tgctgtctgc ttcctgcagc tcgttggagg ccgccaccag
    18841 tagacgcatc gggatttttt ctacgtgtgc accgttgcgg aactggcgct cgttaatggc
    18901 ggtgagcaag gtattaagaa ttgccgggcc cgctttccag atctcatcca gaaagacgat
    18961 ttctgcttcc ggcaggtaac cgctggttaa acgttcatag cgcccttcat cttttagcgc
    19021 ctgaatagaa aggggaccaa aaacttcttc cggcgtggag aagcgggtca tcagatattc
    19081 aaacgcgcgg gcattctgaa aggcgaattt taagcgccgg gcgatcaaac ttttggcaat
    19141 acctggcggg ccaaggagga acacactttc accacttaat gccgctaata aacacaagcg
    19201 gatggcgtgg ctacgttcat aaagcccctt ttccaacgaa ctgctcaggc gggaaattct
    19261 ttccgctaat aaatgagggt gagccataat gaagtggcgt cctttcgtca aaagttctgc
    19321 gtaaattgcg agtatagacg tttcttgctg gtggctaaaa tagtctcaaa g
//
LOCUS       DADLSB010000071        19219 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.071, whole genome shotgun sequence.
ACCESSION   DADLSB010000071 DADLSB010000000
VERSION     DADLSB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.071"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            8..2287
                     /locus_tag="JN331_002359"
     CDS             8..2287
                     /locus_tag="JN331_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7260489.1"
                     /translation="MLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFPMV
                     RKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIREAYGPASIFAGSYGWRSN
                     GVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVVGGSEVYQQQTSWPLVLEH
                     SDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKM
                     EWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKNAE
                     WAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGEQKHWMIVTLAAMLGQIGT
                     PGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDKIPVARIVEALENPGGAYQ
                     HNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIV
                     LPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARF
                     TEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPENPDSERFIRFADF
                     CRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGNAEPEQLQVLSAH
                     PAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRVWNSRGQILAGAV
                     ISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLGNGCAGNTALAWL
                     EKYNGPELTLTAFEPPASS"
     gene            complement(2256..2696)
                     /locus_tag="JN331_002360"
     CDS             complement(2256..2696)
                     /locus_tag="JN331_002360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7260490.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2693..3256)
                     /gene="tag"
                     /locus_tag="JN331_002361"
     CDS             complement(2693..3256)
                     /gene="tag"
                     /locus_tag="JN331_002361"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY7260491.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKAPKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3414..4112
                     /locus_tag="JN331_002362"
     CDS             3414..4112
                     /locus_tag="JN331_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7260492.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4341..5549
                     /locus_tag="JN331_002363"
     CDS             4341..5549
                     /locus_tag="JN331_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7260493.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5873..7564
                     /gene="eptB"
                     /locus_tag="JN331_002364"
     CDS             5873..7564
                     /gene="eptB"
                     /locus_tag="JN331_002364"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY7260494.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDNYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7656..7732
                     /locus_tag="JN331_002365"
     tRNA            7656..7732
                     /locus_tag="JN331_002365"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7690..7692,aa:Pro,seq:cgg)
     gene            8476..10083
                     /gene="dppA"
                     /locus_tag="JN331_002366"
     CDS             8476..10083
                     /gene="dppA"
                     /locus_tag="JN331_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY7260495.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVSPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10391..11410
                     /gene="dppB"
                     /locus_tag="JN331_002367"
     CDS             10391..11410
                     /gene="dppB"
                     /locus_tag="JN331_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY7260496.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11420..12322
                     /gene="dppC"
                     /locus_tag="JN331_002368"
     CDS             11420..12322
                     /gene="dppC"
                     /locus_tag="JN331_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY7260497.1"
                     /translation="MSQVTENKVISAPVPMAPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12333..13316
                     /gene="dppD"
                     /locus_tag="JN331_002369"
     CDS             12333..13316
                     /gene="dppD"
                     /locus_tag="JN331_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY7260498.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13313..14317
                     /gene="dppF"
                     /locus_tag="JN331_002370"
     CDS             13313..14317
                     /gene="dppF"
                     /locus_tag="JN331_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY7260499.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14347..15618)
                     /locus_tag="JN331_002371"
     CDS             complement(14347..15618)
                     /locus_tag="JN331_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7260500.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16094..16201
                     /locus_tag="JN331_002372"
     CDS             16094..16201
                     /locus_tag="JN331_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY7260501.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16288..17967)
                     /gene="bcsG"
                     /locus_tag="JN331_002373"
     CDS             complement(16288..17967)
                     /gene="bcsG"
                     /locus_tag="JN331_002373"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY7260502.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17964..18155)
                     /gene="bcsF"
                     /locus_tag="JN331_002374"
     CDS             complement(17964..18155)
                     /gene="bcsF"
                     /locus_tag="JN331_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY7260503.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(18152..>19219)
                     /gene="bcsE"
                     /locus_tag="JN331_002375"
     CDS             complement(18152..>19219)
                     /gene="bcsE"
                     /locus_tag="JN331_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7260504.1"
                     /translation="FGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLV
                     QSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSL
                     QQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPL
                     SRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLP
                     AHGKGVLVALRPVPGIRVEQAQTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALN
                     HIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDG
                     RHWRRIPEPMRLLDDAVERSS"
BASE COUNT         4585 a         4818 c         5163 g         4653 t
ORIGIN      
        1 ggggcccatg ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg gcgcgttagc
       61 cacaggaatg gaaaactcct tgcagagcgc ggttcgcgac caggttcaca gcaatacgcg
      121 ggtacgattt ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc cgcaaggcat
      181 tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc ttattcacca
      241 acaacataaa cgcattcgtg aggcttatgg tccggcatcg atttttgctg gttcctacgg
      301 ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct atatggcgct
      361 ggcaggcggt tataccgggc atctggggga ttattcgacc ggtgcggcac aggcgatcat
      421 gccgtatgtc gtaggtggca gcgaagttta tcaacagcaa accagttggc cgctggtgct
      481 ggaacatagc gatgtcgtgg tgttgtggag tgctaaccca ctcaatacgc tgaaaattgc
      541 gtggaatgca tccgatgagc aggggctttc ttacttttcc gcactgcgtg acagtgggaa
      601 aaagctgatc tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat
      661 ggagtgggtg gcaccgcaca tgggcaccga tgttgcgctg atgctgggga tcgcccatac
      721 gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca caggttatgc
      781 cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gcgaaaaacg ccgaatgggc
      841 ggcagagatt tgtggtgttg gcgcagcgaa aatccgcgag ctggcggcta ttttccacca
      901 aaataccacc atgctgatgg caggctgggg aatgcagcgc caacagtttg gtgagcaaaa
      961 acactggatg atcgtcacgc tggcagcaat gttggggcaa atcggcacac ccggcggcgg
     1021 ttttggtctt tcttaccatt ttgccaatgg tggtaacccc acgcggcgtt ctgcggtgct
     1081 ctcttccatg cagggcagct tgccgggtgg ctgcgatgcg gtggataaaa tccctgttgc
     1141 ccgcattgtt gaagcactgg aaaaccctgg tggcgcatat caacacaacg gtatgaaccg
     1201 acatttcccg gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca
     1261 ggataccaat cgtctgatcc gtgcctggca aaaaccggag ctggtggtga tctctgaatg
     1321 cttctggacg gcggcggcga aacacgcgga tatcgttctg cctgcgacta cctcttttga
     1381 gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaagca
     1441 agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttgagtga
     1501 acgttgggag aagggcggct atgcgcggtt tacggaagga aaaagtcagc tgcaatggct
     1561 ggaaacgttt tataacgttg cccggcagcg cggggcaagc cagcaggttg aattgccgcc
     1621 atttgctgag ttctggcaag ccaaccagtt aattgagatg ccggaaaacc cggacagcga
     1681 gcggtttatt cgcttcgctg atttttgccg cgatccgctg gcgcatccgt taaaaaccgc
     1741 cagcggcaag attgaaatct tctcacagcg tattgccgat tacggttacc cggattgccc
     1801 tgggcatcca atgtggctgg agccggacga atggcagggc aatgccgaac cagaacagtt
     1861 gcaggtactt tctgcccatc cggcgcaccg cctgcacagc cagctgaatt acagttctct
     1921 gcgcgaattg tacgcggtag caaaccgtga gcctgtcacc attcatcctg acgatgctca
     1981 ggcgcgcggc ataaccgaag gggatatggt gcgggtgtgg aactcccgtg ggcagatcct
     2041 tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgcattc acgaaggggc
     2101 atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct
     2161 gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata cggcgctggc
     2221 atggctggaa aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag
     2281 ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa
     2341 ccctgcgcac ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga
     2401 tggggatagc gctgctgcac atactgcatc agcgccttac caataccgcg tctgacggcc
     2461 ttcggtgcga caaacattgc tgccagaaat cggccttcca taatgctgac aaaaccgaga
     2521 agcttaccgt cctcttccca gacccagttt tgcgcgttgg caagataggc atcccgcacc
     2581 agcggaatgc agtcacgcca gtaattcgct tttataaagg gatgccccca ggttgtactt
     2641 tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt
     2701 tatttcccgg atagcaacag cagccaacca catgatcatt caccagccca catgcctgca
     2761 taaaggagta acagattgtg gtgccgacaa acttaaaacc acgttttttc ggtgccttag
     2821 atagggcgtc ggaggcggac gtagatgtgg gaatttcgct caacgttgtg gcttgtgtca
     2881 cctgtggctg atgatttaca aacgaccaga caaagtcggg aaacggttcg ccgttctgtt
     2941 ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttccct cgatggcgga
     3001 taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgct gcgaccttca
     3061 ccggatcgaa ctgatgaaag caggcgcgat agttttcgcg ttttttgagg acggtgatcc
     3121 acgataaccc agcctgctgc ccttcaaggc agatcatttc gaacagtttt ttactgtcag
     3181 tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggtcc tgactcaccc
     3241 agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt
     3301 ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct
     3361 tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca
     3421 taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagcgtggta atcagtgcct
     3481 tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg caacccggac
     3541 attccacaga gcgttacacc tgggatagtg atcatcaacc tgattacaac gatattttgt
     3601 cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa
     3661 ctcctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg
     3721 ttacaaccgg cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg
     3781 agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtagct
     3841 taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata
     3901 accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga
     3961 caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa
     4021 atattgccgc ctatgctgcg ctaactcagg cagaaaatac cactaataat agcgactatc
     4081 tgtatacgat gggggttagc gccagatttt aacgtaacag tcacaattga aaccattgaa
     4141 taacaacagt tgtggcgata gtgggtgcta acttatcaaa taataaattt ggtgaataat
     4201 tgtcgcgtca ttcattcctg aactaaggca tttcattccg ttctgatggc atttcatgcc
     4261 gtttttcccc aggcataaaa tgcacttcgt tatggttgtc ggcagagatt tttccttttt
     4321 attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc tggctgacac
     4381 tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacctgg agcctgttta
     4441 atggcgcgct ttctgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct
     4501 tgttaagtct ggggctggca atttcgtctt ctgttgcggg caaattgcag gaacgttttg
     4561 gcgttaaacg cgtcaccgtg gcttccggca ttttgctggg attaggcttc ttcctgaccg
     4621 cgcattccaa caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg
     4681 atggcgcggg ttacctgctg acgctctcta actgcgtgaa gtggttcccg gagcgtaaag
     4741 gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta
     4801 tcgacacgca gctgctcgaa acggtgggtc tggaaaaaac ctttgtgatt tggggagcga
     4861 ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag
     4921 tgaaaaccag caatggtgtg gtggagaaag attacacgct ggcagagtcg atgcgtaaac
     4981 cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga
     5041 ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tccgcagcca
     5101 atgcagtaac ggttatttcc atcgctaacc tttcaggtcg tctggtgctg ggcattctgt
     5161 ctgacaaaat tgcccgtatc cgtgttatca ccattggtca ggtgatttcg ctggtgggta
     5221 tggcggccct gctgtttgca ccattaaatg cagtgacgtt ctttgcagcg attgcctgcg
     5281 tggcatttaa ctttggcggc actattaccg tctttccatc actggtcagt gagttcttcg
     5341 gcctcaataa cctggcgaaa aactacggtg tgatttacct cggtttcggt atcggtagca
     5401 tttttggttc gattatcgcc tcactgtttg gcggtttcta tgtgaccttc tacgtgattt
     5461 tcgccctgct gattctgtca ttggcgcttt ctacgacgat tcgtcagcca gagcagaaaa
     5521 tgttgcgtga ggcgcatggc tccctttaat gctcagccgc gagctactgg gcttcagctt
     5581 ccgtgtgcat caaacgcatc aacgaaatgt ataaacaatg actcgtaaaa tttgggccag
     5641 ttaactggcc ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt
     5701 tgtaaatttg tgcttcatgc acactctttc cccacacttt ttcgctttgc tgtggtctac
     5761 ttattcgcgc gtgtagattt tacttatctg actacctccg cacttttccc ctgccgggcc
     5821 tgaaaagccg ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt gcatgagata
     5881 catcaaatcg attacacagc agaagctgag ctttttgctt gcaatctata ttggcctttt
     5941 tatgaatggc gcggtttttt atcgccgctt cggcagctat gcgcacgatt ttaccgtctg
     6001 gaaaggcgtt tctgctgttg ttgaactggc cgccaccgta ctggtgacct tctttttact
     6061 acgtcttcta tcgctgtttg gccgccgcag ctggcgtatt ctggcgtcgc tggtggtgct
     6121 cttttccgca ggtgccagct attacatgac cttccttaat gtggtcattg gttatggcat
     6181 catcgcttcc gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt
     6241 tattctctgg ttaatcgccg ttagtgcatt gcctcttatc cttatctgga ataaccgctg
     6301 tcgctacacc ttgctccgac aactgcgaac cccagggcag cgtattcgca gcctggcggt
     6361 cgtcgtactg gcgggtatta tggtttgggc accgattcgt ttgctggata tccagcagaa
     6421 gaaagtggag agggcgaccg gcgttgattt gccgagttat ggcggtgtcg tagcgaactc
     6481 ttatctgcca tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga
     6541 atcttccgat aataattcat tgcttaatcc ggcgaagaag tttacatacc aggctccaca
     6601 aaacgttgat gatacttatg tcgtgtttat catcggtgaa accacgcgtt gggaccatat
     6661 gggtattttc ggctatgagc gtaataccac gccgaaactg gcccaggaga aaaatctggc
     6721 ggcgttccgt ggttactcct gtgataccgc aaccaaactc tcactgcgtt gcatgtttgt
     6781 acgtcagggg ggcgcggaag ataatccgca gcgcacatta aaagaacaga acattttcgc
     6841 ggttctgaag cagttaggat tcagttctga cctctacgct atgcagagcg aaatgtggtt
     6901 ctacagcaac acgatggcgg acaacattgc ttatcgtgag cagattggtg cggagccacg
     6961 taatcgtggc aagccggtag atgatatgtt gctggtagat gaaatgcagc aatcgctagg
     7021 gcgcaacccg gatggtaagc atctgatcat tctgcatacc aaaggttcgc attttaacta
     7081 cacccagcgt tatccgcgta gcttcgcgca gtggaagccg gaatgtattg gtgttgatag
     7141 cggctgtacc aaagcgcaga tgatcaactc ctatgacaac tcggtgacct atgtggatca
     7201 ctttatctcc agcgtgattg atcaggttcg cgataagaaa gcgattgtgt tctacgcagc
     7261 tgaccacggt gagtcaatta atgaacgcga gcacctgcac ggcacgccgc gtgaactggc
     7321 accgccggag cagttccgcg taccgatgat ggtctggatg tcagataact atctggaaaa
     7381 tccggccaat gcgcaggcgt ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg
     7441 ccgtcacgta gagctgtacg acaccatcat gggttgtctt ggctatactt caccggatgg
     7501 tggaattaac gaaaacaaca actggtgtca catcccgcag acaaaagtgg cagcggctaa
     7561 ctaagcggcc tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa gggattgacg
     7621 agggcgtatc tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag cctggtagcg
     7681 cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa
     7741 aagcctgctc aacgagcagg tttttttgca tttgtagctc atgaggatga gaacctccgg
     7801 gggcagggag gctcgactcg acgtaggcct gataagacgc gccagcgtcg catcaggcaa
     7861 cggcaccgca cttattgtcg gatgcggcgc gaccgcctta ttcgaccaac acaccacaca
     7921 ttttcctgcc aactttgtgc cccatatctc actatccatt aacggatttg tgacagaaac
     7981 cattgtcttt ttttatattt gcttaaatct tttttgcgtt acttatggct ggcgctggca
     8041 ttttccgctg tgcgttttag cgttcatggt attaatcgct cagatatcca ccttttcctc
     8101 agattttttt cattaaatgc ataaaactta atcaccaaat gttgctaaat attaagctga
     8161 ttgttctggt tatgttggag tagcacaaat ttctctgctg caaatagctg tttgaagttt
     8221 ttttaatctt tgtttgtgga ttctcaccgt tggtgtaaat cccggttggc tgtattgacg
     8281 ttttcacatt ctgttgacag attgtaggtc acgaggggca ttttatggag gatccgcact
     8341 gttacactga tgttaattag tacggcatcc ccacctcata acgttgaccc gaccgggcaa
     8401 aaaacaaaaa aggtcaggca gcgacaaccc attgcaaagg gttaaaacaa caaacatcac
     8461 aattggagca gaataatgcg tatttccttg aaaaagtcag ggatgctgaa gcttggtctc
     8521 agcctggtgg ctatgaccgt cgcagcaagt gttcaggcta aaactctggt ttattgctca
     8581 gaaggatctc cggaagggtt taacccgcag ctgtttacct ccggcaccac ctatgacgcc
     8641 tcttccgtcc cgctttataa ccgcctggtt gaatttaaaa tcggcaccac cgaagtgagc
     8701 ccgggcctcg ctgaaaagtg ggaagtcagc gaagacggta aaacctatac cttccatctg
     8761 cgtaaaggtg tgaagtggca cgacaataaa gaattcaaac cgacgcgcga aatgaacgcc
     8821 gatgacgtgg tgttctcgtt cgatcgtcag aaaaacgcgc aaaacccgta ccataaagtt
     8881 tctggcggca gctacgaata cttcgaaggt atgggcttgc cggagctgat cagcgaagtg
     8941 aaaaaggtgg acgataacac cgttcagttt gtgctgactc gaccggaagc gccgttcctc
     9001 gctgacctgg caatggactt cgcctctatt ctgtcaaaag aatatgctga cgcgatgatg
     9061 aaagccggta caccggaaaa actggacctc aacccaatcg gaaccggtcc gttccagtta
     9121 cagcagtacc aaaaagattc ccgtatccgc tacaaagcgt ttgatggcta ctggggcacc
     9181 aaaccgcaga tcgatacgct ggtcttctct attacccctg atgcttccgt gcgttacgcg
     9241 aaattgcaga agaatgaatg ccaggtgatg ccgtacccga acccggcaga catcgctcgc
     9301 atgaagcagg ataaatccat caacctgatg gaaatgccgg ggctgaacgt cggctacctc
     9361 tcgtataacg tgcagaaaaa accgctggat gacgtgaaag ttcgccaggc actgacctac
     9421 gcggtgaaca aagacgcgat catcaaagcg gtttatcagg gcgcgggtgt atcagcgaaa
     9481 aacctgatcc cgccaaccat gtggggctat aacgacgacg ttcaggatta cacctacgat
     9541 cctgaaaaag cgaaagcctt gctgaaagaa gcgggtctgg aaaaaggttt ctccatcgac
     9601 ctgtgggcga tgccggtaca acgtccgtat aacccgaacg ctcgccgcat ggcggagatg
     9661 attcaggcag actgggcgaa agtgggcgtg caggccaaaa tcgtcaccta cgaatggggt
     9721 gagtacctca agcgtgcgaa agatggcgaa catcagacgg taatgatggg ctggactggc
     9781 gataacgggg acccggataa cttcttcgcc accctgttca gctgcgccgc ctctgaacaa
     9841 ggttctaact actcaaaatg gtgctacaaa ccgtttgaag atctgattca accggcgcgt
     9901 gctaccgacg accataacaa acgcgttgaa ctgtacaagc aagcgcaggt ggtgatgcac
     9961 gatcaggctc cggcactgat catcgctcac tccaccgtgt ttgaaccggt acgcaaagaa
    10021 gtcaaaggct atgtggttga tccattaggc aaacatcact tcgaaaacgt ctctatcgaa
    10081 taattaaaag ccatacaaga ctgatggcaa aggcaaaact gcctgatgtg ctccgcttat
    10141 caggcctacg aaaattctgc aatgtattga atttgcacga ttttgtaggc cggataaggc
    10201 gttaacgccg catccggcat aaacaaagcg cactttgtca acaatctgta tacccggtgg
    10261 cgctgtgcct ctgcgtgcag cgccatccgg cagcaatact tcattctctg tccgacaagg
    10321 gcgggaattg atttgtgagc aatacagaca cgcagttcca ggctgcgggt cactatagag
    10381 aatccgggtt atgttgcagt ttattctccg acgtttggga ctcgtcatcc ccacgtttat
    10441 cggtattacc cttctcacat ttgcctttgt ccacatgatc ccgggcgatc cggtgatgat
    10501 catggcaggc gaacgtggga tctcccctga gcgtcacgtg cagctgctgg ctgaactcgg
    10561 cttagataaa ccgatgtggc agcagtatct ccattacatt tggggcgtaa tgcacggcga
    10621 tctaggcatt tcaatgaaaa gccgaattcc agtatgggaa gagttcgtgc cgcgctttca
    10681 ggccacgctg gaacttggcg tctgcgcgat gatttttgct acggcagtcg gtattccggt
    10741 tggtgtgctg gctgcggtta aacgcggttc cattttcgat cacactgcag ttggcttggc
    10801 gctgaccggc tactcgatgc cgatcttctg gtggggcatg atgctgatca tgctggtttc
    10861 ggtgcactgg aacctgacgc ccgtctccgg tcgcgtgagc gatatggtgt tcctcgatga
    10921 ctccaatccg ttaaccggtt ttatgctaat cgacaccgcc atctggggtg aagacggcaa
    10981 ctttatcgat gccgtcgccc atatgatctt gcccgccatt gtgctgggta ctattccgct
    11041 ggcggtcatt gtgcgtatga cacgctcctc gatgctggaa gtgctgggcg aggattacat
    11101 ccgcaccgcg cgcgccaaag ggctaacccg catgcgggtg attatcgtcc atgcgctgcg
    11161 taacgcgatg ctgccggtag tgaccgttat cggcctccag gtgggaacat tgctggcggg
    11221 ggcgattctg accgaaacca tcttctcgtg gcccggtctg gggcgctggt tgattgacgc
    11281 actgcaacgc cgcgactatc cggtagtgca gggcggcgta ttgctggtgg cgacgatgat
    11341 tatcctcgtc aacttgctgg tcgatctgct gtacggcgtg gtgaacccgc gtattcgtca
    11401 taagaagtaa ggggacatca tgtcacaggt tactgaaaat aaagtgatta gcgcaccggt
    11461 gccgatggcc ccgttacagg agttctggca ctattttaaa cgcaacaaag gcgcggtcgt
    11521 cgggctggtt tacgtcgcca tcgtgctgtt catcgcgatc tttgccaact ggattgcgcc
    11581 ttataacccg gcggatcagt tccgcgatgc gctgctcgcc ccgccagcct ggcaggaagg
    11641 cggcagtatg gcacacttgc tgggcaccga tgacgtcggt cgtgatgtgc tgtcgcggct
    11701 gatgtacggc gcgcgtttat cgctgctggt tggctgtctg gtggtggtgt tgtcgctgat
    11761 tatgggcgtt attctcggcc tgatcgccgg ttacttcggc ggcctggttg ataacattat
    11821 catgcgcgtg gtcgatatca tgctggcgct gccaagtctg ctgttggcac tggtgctggt
    11881 ggcgattttc ggcccgtcga ttggtaacgc cgcgctggcg ctgaccttcg ttgccttgcc
    11941 gcactatgtg cgcttaaccc gcgccgccgt gctggtggag gtgaatcgcg attacgtcac
    12001 cgcctctcgc gtggcaggtg ccggggcgat gcgccagatg tttattaaca tcttcccgaa
    12061 ctgccttgcg ccgctgattg ttcaggcgtc gctcggtttc tctaacgcca ttctcgatat
    12121 ggctgctctt ggtttcctcg gcatgggggc acagccgcca acgcctgagt ggggcaccat
    12181 gctctccgac gtgttgcagt tcgcgcaaag cgcctggtgg gtcgtgacct tccctggtct
    12241 ggcgatcctg ctgacggtgc tggcatttaa cctgatgggt gacggtctgc gtgacgcgct
    12301 cgatcccaaa ctgaagcagt aagaggttcg agatggcgtt attaaatgta gataaattat
    12361 cggtgcattt cggcgacgaa agcgcaccgt tccgcgccgt agaccgcatc agctacagcg
    12421 taaaacaggg cgaagtggtc gggattgtgg gtgagtccgg ctccggtaag tcggtcagtt
    12481 cactggcgat tatggggctg attgattatc cgggccgcgt aatggcagaa aaactggagt
    12541 ttaacggcca ggatttgcag cgtatctcag aaaaagagcg ccgcaacctg gtgggtgccg
    12601 aagtggcgat gatcttccag gacccgatga ccagccttaa cccgtgctac accgtgggtt
    12661 tccagattat ggaagcgatt aaggtgcatc agggcggcaa caaaagtacc cgccgtcagc
    12721 gggcgattga cctgctgaat caggtcggta ttcccgatcc ggcttcgcgt ctggatgttt
    12781 acccgcatca gctttccggc ggcatgagcc agcgcgtgat gatcgccatg gcgattgcct
    12841 gtcggccaaa actgctgatt gccgatgaac cgaccaccgc gctggacgtg accattcagg
    12901 cgcaaatcat cgaactactg ctggagctac agcagaaaga gaacatggcg ctggtgttaa
    12961 ttacccatga cctggcgctg gtggcggaag ctgcgcataa aatcattgtg atgtatgcag
    13021 gtcaggtagt agaaacgggc gatgcacacg ccatcttcca tgcgccgcgt cacccgtata
    13081 ctcaggcatt gctgcgtgcg ctgccagaat ttgctcagga caaagaacgt ctggcgtcgt
    13141 tgccaggtgt tgttcccggc aagtatgacc gcccgaacgg ctgcctgctt aacccgcgct
    13201 gcccctatgc cactgacaga tgtcgcgctg aagaaccggc gctgaatatg ctcgctgatg
    13261 ggcgtcagtc caaatgccat tacccacttg atgatgccgg gaggcccaca ctatgagtac
    13321 gcaagaggcc acctcgcaac aaccgctgtt gcaggctatc gacctgaaaa aacattatcc
    13381 ggtgaagaaa ggcatgttcg tgccggaacg tctggtgaag gcgctggacg gcgtttcgtt
    13441 taaccttgaa cgtggcaaaa cgctggcagt agtgggcgaa tctggctgcg gtaaatcgac
    13501 cctcggtcgg ttgctgacga tgatcgaaac gcccaccggc ggcgagctgt attatcaggg
    13561 gcaggatctg ctcaagcacg atccgcaggc gcagaagctg cgtcggcaga aaatccagat
    13621 cgtcttccag aatccctacg gttcgctgaa tccgcgtaag aaagttgggc aaattctcga
    13681 agagccgctg cttattaaca ccagcttaag caaagaacag cgtcgggaaa aagccctgtc
    13741 gatgatggca aaagtcggcc tgaaaaccga gcactatgac cgctatccgc acatgttttc
    13801 cggcggacaa cgtcagcgta tcgctattgc ccgtggtctg atgctcgacc cggatgtggt
    13861 gattgccgat gagccggttt ctgcgctgga cgtctcagtg cgtgcgcagg tgctgaatct
    13921 gatgatggat ttgcagcagg agttggggct ttcttatgtc tttatctccc acgacctgtc
    13981 ggttgtggag cacattgctg atgaagtgat ggtgatgtac ctaggccgct gcgtggagaa
    14041 aggaacgaag gaccaaatct tcaataaccc gcgccatccg tacacccagg cgctgctctc
    14101 cgcgacgcca cgcctgaacc cggacgatcg tcgtgaacgc atcaaactca ccggtgaact
    14161 gccaagcccg ctgaatcctc cgccgggttg cgccttcaac gcccgctgtc gtcggcgctt
    14221 cggcccctgc acccagttgc agccgcagct aaaagactac ggcggtcaac tggtagcttg
    14281 ttttgctgtt gatcaggatg aaaatccgca gcgttaactc acaaaaccgg ggccatgcgc
    14341 tccggtttac tcaatcagct tcaacagtgg cgacaagcat agcaaaatgc cgtaaagcag
    14401 aatcagccag gctttaaatc cgcgaagttt ttccagttgt gcgactttat agatcaggaa
    14461 aaacggaatg aggcacgaca caatgccata taacgggctt cccaactgaa agaacaccag
    14521 cactgataca cgaaacgaaa cccaaatcgt caacgtaatg acgataaaag cgcagatcgc
    14581 cagagtcagc atgcgtgagt taattttctt ggtatcaata attcggctta acagattgag
    14641 aataatgcct ttaatagcct cgtggaaacc gagataaatg ccaaagaatg cggtcagtac
    14701 ggcaaagata ttcagcaccg tagaggtgat atgaatgata tgtccaggga tcacctgcgc
    14761 ggccagtgcc agtgccgaga tattttgttc aaaggcagaa acggcttctt cgtggctaat
    14821 tgagaaggta aacgaaaagg caaaaaacag gatcaccgcg atgagcgtga tataactaat
    14881 tcggtgggta cgcagcgcga gccgggttgc cagtacttta tctgcttccc gtttacgata
    14941 ggcaatattc attgggttaa gtacctgaat aaataccgca gaaaagaagc aaaatggaat
    15001 ggtaagcagg acatcgcgga aaaagacgga ggcttgcggg aaggcggtta tattggcgaa
    15061 attccagtgc gggatcatcg caaaaccgaa cacgacaata atccctactt tgaccaccac
    15121 cattggccca gaaatcttaa acagtaatcg ttcaccacca gacgcaatcg ctatcagtac
    15181 ggcgaaaata gcgactttat aaagtagaga ttgtgaaaga tcggcatcgg ttaaaccgaa
    15241 ggttttcagg tacgaggcgc tgtcgaaaac cacggagaga gagtagataa aaatcccgtg
    15301 gataatcatc aaaaagtaga taaccccgag gaaaattccc cagttcttcc ccaggtaatg
    15361 actgataata tcggtgtagt cattacagga atcgctttca gaaagggttt ttaaataaat
    15421 gtcctgcact acccaggtgg caggataagc aatgatcgct gcggtaataa atacccaaat
    15481 tcccttcaag ccaatttgta ctggcatcag cacggttccg gcaccaatcg ccatgcctat
    15541 gcataataaa acccagccga agtcgtagcg tgtaaacggc aatttctgat tgtgtttcga
    15601 tagtgtgttg tgctgcataa agtcctgtcc agaaagaagt cggcctcgca aaaatgaata
    15661 aacccagttt ctcttgcgca acaggtggag aagtcgaata cgcagtatgt aaaaaatgag
    15721 gcaacagatt cactggggag gtcgcgagga ggcgcaattc tacaaaattg cgcatttcac
    15781 taccagtctg gagtcagccc ggatatgaac tgcaactatt gcaaataaag aaattaagct
    15841 ggaaggcatc gcgcaatgag gaagattgcc gggtgctgac agtaaaaagc aatcgtagaa
    15901 tcagcggcaa gccggaaagg ttccggtgag gcgcaatgtt gcgggggctt tatccctggt
    15961 ggcattggtt gctggagaga gaaaaccccc gcacgttgca ggtatgcacc tgacaacatc
    16021 acgggggcta atcttgaacc caaacaactc aagaatagcc gcgaaacgtt ggcattacaa
    16081 cacaggcggc tatatgacgc tcgcagaact gggcatggcc ttctggcatg atttagcggc
    16141 tccggtcatt gctggcattc ttgccagtat gatcgtgaac tggctgaata agcggaagta
    16201 acgtgtcatg cgggcgtcag gctgccgtaa tggcaatttg cgcccggatc aggccgcaga
    16261 ggttaaactc tgcggccttt ttcgtgctta ctgcgggtaa ggcacccagt cgccgccgtt
    16321 caggcgaacg tacggtttat cttggtattg aataactact gcatttgagt tctcggagac
    16381 cggtgctgtt tgtggcaacc cactggtgag ttttttccag tcaacattgt cttcggtgaa
    16441 aatcttgcca tcaagaacgc gaaccaccag atcggagata gccaggaagc tgctcggttg
    16501 gtcaatgaca atcggtgccc cctgatgtgg tgccttcatg ccgaagaatt tcaccccaac
    16561 ggggacgtct gtgatagacg ggctagggat atcacgtagg ccagatacct gcattctgtc
    16621 gcccttcagc gcgccgccgt gttccggcac cacgaccacc atcactttac gacccgattt
    16681 ctccagttca gtaaagaagg cgtccagttc atcaaagaat ttctgcgccc gcgctttgta
    16741 atccgctgtt ttgctgaccc ccggataatg gttgccgtca tgcagtggaa gcgtgttgta
    16801 gaacgtggca ctacggctgt ttttatcttt ttcggtaacg tccagccagc ggttaagcac
    16861 ggcggtatcg tcataaaccg gcgaaccatc aaagcccagc aaaataaccg gcagatttgt
    16921 ttgatccatc aattcagtct gcatgccgcc attttcgcga acttctttca aaaaaccgcc
    16981 gaactggccg ttatgtccca tcatcaggtg ctgggtaaag cccagtttcg aaaggttatc
    17041 aaacagatag cagtcgttat ttgccggttg atacagatta gtgtgcgaag tctgcccgca
    17101 gctggcgcgc agtaaacgga tcgccgccgg gccactgtag gaggtggcgg agttaaagtt
    17161 cttgaactca atatcgaaat gcgaccacag tggatgcgac atcaacccgg cggcttctat
    17221 atccgaccag gaaagcgaac agatgttaat caccagtagt tcaaatggct gagcatcagc
    17281 gggcagcgaa gacgggaagg tcgatttacg tttcgcctcc gcgttataga aattattcag
    17341 ccaggcgtta aggttcgccg ttgttggcgg tgcagtttgt gcgggcatat cacccactac
    17401 cggtgcgcca cccgtcgccg caacggttgc cgctgcgtta ccacccgtcg ttgttacagt
    17461 ggtcgtcggt tgtccggctg gccacaagga gaaacttggt cccgccaggg taagtacgtt
    17521 cagccatagc agtatggcaa ccacaaaaac ggtaatgcga atccattgtg acaggaataa
    17581 ccaggccact aataaaacaa aaatggcccc aatcatctgc cagttaataa agcgtgtgac
    17641 aaggtcgatt aaataatcgg tactgaaccc cgccacctgc gaaccctggc tcattatgct
    17701 ttccgggcca ggcaaccagg tgtcatgcca gaacaaagca aagccgatcg gcagggcaat
    17761 ccagtggcgc aagcgatgca ggctgtaacg cggaatgggc atcagcagaa acgcggcaaa
    17821 caccaaattg aggagcgggt ggaagttaag atatcccgcc cacaacaggc caaacttaac
    17881 cagaaaatag aagttccagc cggaaaggcc gcgccagtat tgccagaggg aagaaggcat
    17941 ggcggtattt tgcgtaaatt gagtcatttt ttggttgccc tggctttttc cgtgcggcgt
    18001 aacgtcccgg ccggtttaac ataacgaggt ttagcaaaga acaaacgtaa ggtgtcgcga
    18061 atgcgtcgca aagagtgccg cgccagatag cccagcggga aaaatatcag tgcgcaaacg
    18121 acaataattt caatgatatc gctgatggtc atcatgatga gcgttccaca gcatcatcta
    18181 acagtcgcat gggttctggt attcgtcgcc agtggcgacc atcgtgctcg gcattgatga
    18241 ccggtttaga actttgcgtt aaaggcagcg gcattcccca ttgttctggg gcaagcaggc
    18301 gcatctgcac cagctcggca ctgatttgat catcttcaaa ccagaccata cggtttgaga
    18361 aaatgtcgcc agtaggcaat gggaaaatat gattcaacgc ggtatccaga tcgttaatcc
    18421 gacagaatga gagaaacagc accagccgat taccgccaat ggtcatgata tcgccggtac
    18481 ggttagggcg acatagcgtc tgggcttgct caacgcggat acccggtacc ggacgtaggg
    18541 caaccagaac gcctttaccg tgggcaggca agagagggtt attcatcatg ttgttgacgg
    18601 cattacagaa cacatcccac ttctggaaac cacgcagttt gagcggctgg gtcattgaca
    18661 gcaaggtagt gatatcttcc ggtacatagc gactaaactt ctgcccttgc acgctttcga
    18721 tcatcgtcag acaacgggag agtggcgcat tccacggaat aaccatattt gcaccgcagg
    18781 ccaataacaa acgttcatcg gtggcgcgca ggctagcggt attttcccgc acgaggattt
    18841 tcatcgcact accgcgctgg cgacgcaggg tatgaatgct gcgggccagt ggctcgattt
    18901 gcgcattttg ctgtaaagaa aagaccaccg tcgccgcctg agcggtacgg gcttcattga
    18961 acaggacttc gttattgttg aacagttgcc agtgttccga tagcggcggc gcaccttcca
    19021 gtacagcaac attactcaga atgcgttttt cgtcgctgcg tggttggatc tccgcctctt
    19081 cgctttgaac taatgtccag ataccatttt gttgctgaac gctaagctgc tgacgggcgc
    19141 tgaccccttt ttcgttgcac cagaaggcaa tatccagcaa atgttggtca ccctgaaaac
    19201 gcaaactggc aagaccaaa
//
LOCUS       DADLSB010000072        18985 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.072, whole genome shotgun sequence.
ACCESSION   DADLSB010000072 DADLSB010000000
VERSION     DADLSB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.072"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..378)
                     /locus_tag="JN331_002376"
     CDS             complement(<1..378)
                     /locus_tag="JN331_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7260505.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQM"
     gene            746..1876
                     /locus_tag="JN331_002377"
     CDS             746..1876
                     /locus_tag="JN331_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY7260506.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            1876..2814
                     /gene="fruK"
                     /locus_tag="JN331_002378"
     CDS             1876..2814
                     /gene="fruK"
                     /locus_tag="JN331_002378"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY7260507.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            2831..4522
                     /gene="fruA"
                     /locus_tag="JN331_002379"
     CDS             2831..4522
                     /gene="fruA"
                     /locus_tag="JN331_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY7260508.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCADMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            4946..5887
                     /locus_tag="JN331_002380"
     CDS             4946..5887
                     /locus_tag="JN331_002380"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY7260509.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGKNGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            5875..6813
                     /locus_tag="JN331_002381"
     CDS             5875..6813
                     /locus_tag="JN331_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY7260510.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            6907..8157
                     /locus_tag="JN331_002382"
     CDS             6907..8157
                     /locus_tag="JN331_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7260511.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAILPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8228..8926)
                     /gene="yeiL"
                     /locus_tag="JN331_002383"
     CDS             complement(8228..8926)
                     /gene="yeiL"
                     /locus_tag="JN331_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY7260512.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKHLSGLA
                     LEMDPENKFSGMMQ"
     gene            9057..9998
                     /gene="rihB"
                     /locus_tag="JN331_002384"
     CDS             9057..9998
                     /gene="rihB"
                     /locus_tag="JN331_002384"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY7260513.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVVGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10098..11348
                     /locus_tag="JN331_002385"
     CDS             10098..11348
                     /locus_tag="JN331_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7260514.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(11404..12493)
                     /locus_tag="JN331_002386"
                     /pseudo
     CDS             complement(11404..12493)
                     /locus_tag="JN331_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(12496..13353)
                     /gene="nfo"
                     /locus_tag="JN331_002387"
     CDS             complement(12496..13353)
                     /gene="nfo"
                     /locus_tag="JN331_002387"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY7260515.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(13427..14476)
                     /locus_tag="JN331_002388"
     CDS             complement(13427..14476)
                     /locus_tag="JN331_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY7260516.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSTLKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            14575..15456
                     /locus_tag="JN331_002389"
     CDS             14575..15456
                     /locus_tag="JN331_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7260517.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGWLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            15662..17131
                     /gene="lysP"
                     /locus_tag="JN331_002390"
     CDS             15662..17131
                     /gene="lysP"
                     /locus_tag="JN331_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY7260518.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            17423..>18985
                     /gene="cirA"
                     /locus_tag="JN331_002391"
     CDS             17423..>18985
                     /gene="cirA"
                     /locus_tag="JN331_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAY7260519.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGIKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYY"
BASE COUNT         4562 a         4546 c         5126 g         4751 t
ORIGIN      
        1 catttgcggg ttagcggtcg agccaaagct gctaagaaag acgccaacga atagcaaaac
       61 aaagtagttg cgattccagg caaaaagggt gcaggccagc acgcctaaca ggcagcaaaa
      121 gacaatcagc gatttgcgat cgccgcgctt atcagagcgc ccagcgagaa actgacttac
      181 cagaatccca atgacagcgc tgccggtaaa gaagaatccc accatcgccg gacgggcatg
      241 tacttcatcg gtaaggaaaa tactgagtgt cggggtttgc agagcacccg caataccggt
      301 gagaaaggca acgattaaaa acgccgtcga ggtcaggtca aacgatttcg cgctggagac
      361 tgcgggggag ttatgcatgc tggtcgatca ctggaagagg aagacgcgga gtttacgctg
      421 cttgtcagaa aataaacaga cagcgaaatc aaaagcgcca cgaataatca aaatgccatt
      481 tcactgttgc tgattggctg ctgacgctgc tgaaatgaaa ttgctgataa cggattttcc
      541 catcagcaat taggaaaaat gacaaaaaat tgtgcagcac atcaaacttt tgctcataac
      601 tttacggctt tccttgcgtg ctgaaacaga agtattatgc tttcttgaaa cgtttcagcg
      661 cgatcttgtc tttaacccta agccaggttg gcgctttttt tctcatagag gctgaatcgt
      721 ttcaattcag caagagagga gaactatgtt ccagttatcc gtacaggaca tccatccggg
      781 cgaaaaggcc ggagacaaag aagaggcgat tcgccaggtc gctgcggcgc tggtgcaggc
      841 cggtaatgta gcagaaggct acgtcaatgg catgctggcg cgcgaacagc aaacctcaac
      901 gttcctcggc aatggtattg ctattccaca cggcactacc gacacccgcg atcaggtgct
      961 gaaaaccggc gttcaggtat ttcagttccc ggaaggcgtc acctggggtg acggtcaggt
     1021 agcgtacgtg gcaatcggta ttgctgccag ctcggataaa catctgggcc tgctacgcca
     1081 gctgacccac gtactgagcg atgattccgt tgctgaacaa ctgaagtcag caacaacagc
     1141 agaagaactt cgcgcattac tgatgggcga aaagcagagt gagcagctga agctcgacaa
     1201 cgaaatgctg acgctggata tcgtcgccag cgatctgctg actcttcagg cgctgaacgc
     1261 tgcgcgtctg aaagaggcga gggcagttga cgccactttc gtcaccaaag ccatcaatga
     1321 acaaccgctg aatctcggac agggtatctg gctgagcgat agcgccgaag gcaatctgcg
     1381 tagtgcgatt gcggtaagcc gtgcggcaaa tgcttttgat gtggacggcg aaacggcagc
     1441 catgctggtg agtgtggcga tgaatgacga tcagcccatc gcggttctta agcgtctcgc
     1501 tgatttgttg ctcgacaata aagctgaccg cttgctgaaa gcggatgcgg caacgttgct
     1561 ggcgctgctg accagcgatg atgcgccgac cgacgacgtg ttaagcgcgg agtttgtggt
     1621 gcgcaatgaa cacggcctgc atgctcgtcc aggtaccatg ctggtcaata ccattaaaca
     1681 atttaacagt gatattaccg tgacaaacct tgatggcacc ggcaaaccgg caaacggacg
     1741 tagtctgatg aaagttgtgg cacttggcgt taagaaaggt catcgcctac gctttaccgc
     1801 ccagggtgca gatgctgaac aggcgctgaa agccattggc gacgctatcg ctgctggtct
     1861 tggggagggc gcataatgag cagacgtgtt gctacgatca cccttaatcc ggcttatgac
     1921 cttgttggtt tctgcccgga aattgaacgc ggcgaagtga acctggtgaa aaccaccggt
     1981 ctgcatgcgg cgggtaaagg catcaacgtg gccaaagtat taaaagacct gggaattgat
     2041 gtcaccgttg gcggcttcct gggtaaagac aatcaggatg gttttcagca actgttcagc
     2101 gagctgggca ttgccaaccg tttccaggtt gtacaggggc gcacccgaat taacgttaag
     2161 ctgacggaaa aagacggcga agtgaccgac ttcaacttct cgggttttga agtcaccccc
     2221 gccgactggg aacgctttgt gactgattct ctgagctggc tcggtcagtt cgatatggtc
     2281 tgtgtcagcg gaagcttacc gtcaggcgtc agcccggaag cgttcaccga ctggatgact
     2341 cgcctgcgta gtcagtgtcc ttgcattatc tttgatagta gccgtgaagc gttagtagca
     2401 ggtttgaaag cggcaccgtg gctggtgaaa cctaaccgcc gcgagttgga aatctgggca
     2461 ggccgtaaac tgcctgaaat gaaagatgtg attgaagctg cacatgcgct acgtgaacaa
     2521 ggtattgcgc atgttgttat ttcactgggt gccgaaggcg cgctttgggt taatgcctcc
     2581 ggcgaatgga tcgctaaacc accgtcagtc gatgtcgtaa gcaccgttgg cgcaggggat
     2641 tctatggttg gtggcctgat ttatggcttg ctgatgcgtg aatccagtga acacacactg
     2701 cgtctggcga cagctgttgc agccctggcg gtaagtcaaa gcaatgtggg tattaccgat
     2761 cgtccgcagt tggccgcaat gatggcgcgc gtcgacttac aaccttttaa ctgacagcag
     2821 gagaggcata atgaaaacgc tgctgattat tgacgctaat ctcggtcagg cacgcgccta
     2881 tatggcgaag accctgctgg gcgcggcggc gcgaaaagca aaactggaaa tcatcgacaa
     2941 tccgaacgac gcagaaatgg cgattgttct cggtgattcc atcccgaatg acagcgcgct
     3001 gaacggtaaa aatgtctggc tgggtgatat ttcccgggca gttgcgcacc ctgagctgtt
     3061 cctgagtgaa gccaaaggcc atgcgaaacc ttacactgcg ccggtcactg cgacagcacc
     3121 ggttgccgcc agcggtccga aacgcgtagt tgcggtgact gcttgcccga ctggcgtagc
     3181 acacaccttt atggcggctg aagccattga aaccgaagcg aaaaaacgtg gctggtgggt
     3241 gaaagttgaa acccgtggtt ctgttggcgc gggtaatgca atcactcccg aggaagtagc
     3301 cgcagcggat ctggtgattg tggcggcaga tatcgaagtg gatctggcga aatttgctgg
     3361 taaaccgatg tatcgtacct ctaccggtct ggcgctgaag aaaaccgcgc aggaactgga
     3421 taaagcggtt gctgaagcaa cgccgtatga accggcgggc aaagctcaaa cggcgaccac
     3481 tgaaggtaag aaagagagtg caggcgctta tcgtcacttg ctaacgggcg tctcttacat
     3541 gctgccgatg gtcgttgctg gtggtctgtg tatcgcgctt tcttttgctt ttggtatcga
     3601 agcgtttaaa gagccgggta cgttggcagc ggcgctgatg cagattggtg gtggttcagc
     3661 ctttgcgctg atggtgccgg tactggcagg ttatattgcc ttctccattg ccgatcgtcc
     3721 gggcctcacg ccgggtctga ttggcggtat gctggcggtc agcaccggtt ctggcttcat
     3781 tggcggtatt attgcgggct tcctggctgg ttacattgcg aagttaatca gtacgcaact
     3841 gaagctgccg cagagtatgg aggcgctgaa accgatcctg atcattccgc taatttccag
     3901 tctggtggtc ggtctggcga tgatctacct gatcggtaaa ccagttgctg gcattctcga
     3961 agggctgact cactggctgc agaccatggg gactgcgaat gcggttctgc tgggggcgat
     4021 cctcggtggc atgatgtgtg ctgacatggg cggtccggta aacaaagcag cgtacgcatt
     4081 cggtgtgggt ctgctgagta ctcaaaccta tggcccgatg gcggcgatta tggcggcagg
     4141 tatggtgcca ccgctggcaa tgggcctggc aacaatggtg gcgcgtcgca aattcgacaa
     4201 agcgcagcag gaaggtggca aagccgctct ggtattggga ctgtgcttca tttcggaagg
     4261 tgcaattccg tttgctgctc gtgatccgat gcgtgtgctg ccgtgctgta tcgtgggtgg
     4321 ggcgctgact ggcgcaatct caatggcgat tggtgcgaaa ctgatggcac cgcacggtgg
     4381 tctgtttgtt ctgctgatcc ctggcgctat tacgccggta ttgggttacc tggtagcaat
     4441 tattgccggt acgctggtgg cgggtttggc ctatgctttc ctgaaacgtc cggaagtgga
     4501 cgcagtagcg aaagcagcgt aataaaaggt gtgttacagg gcagaaattt cctgccctgt
     4561 aacatttgat gagcgcaaag agaatgacca gcttgtgaat gtaattttat gaatttaaat
     4621 ggttttatct ttcagtagaa acttcaaacg ttcatcaaac actaaggtta tttcctcaag
     4681 ggcagattat tttctgccct tttttagtgt tcctgttctg tgcatcccgt cacaaattcc
     4741 tcgcttttgt aattcaggca tttaatcgca ttttgtgagc ctgctcaaat ctgcccattt
     4801 tgcctctctg gcggtaattt actttacgta aaacatgcat actatgagca catgtttaaa
     4861 tatgaacaca tgtttaaacc gtctttgcag tatctatatt cttgtggatt gacagggttg
     4921 gtaatttttt accagaggac gtgctatgcg cgaaaaggat tatgtcgtaa ttataggttc
     4981 ggcgaatatt gatgtcgccg gatattcaca tgaatcatta aattatgcgg attcaaatcc
     5041 aggtaaaata aaatttacgc ctggtggagt agggcgcaat attgcacaaa acctggcgtt
     5101 gctgggtaac aaagcctggc tactgagcgc cgtaggcagt gatttttatg gtcaatcgct
     5161 gctaacgcaa accaatcaat ctggtgttta tgtcgataaa tgcctgattg tgccgggaga
     5221 aaatacgtcg agttatttat cattactcga taataccggt gaaatgctgg ttgctataaa
     5281 tgacatgaat attagcaacg ctattacagc tgaatatctc gcacagcacc gtgaatttat
     5341 tcagagggca aaggtcattg tcgcggactg taatatcagt gaagaggcac tggcatggat
     5401 tctggataat gccgccaacg tacccgtatt tgtcgatccg gtttcagcgt ggaaatgtgt
     5461 caaagtgcgc gaccgcctaa atcagatcca cactctgaag ccaaaccgcc ttgaagcgga
     5521 aaccctgagt gggattgcgc tgtcagggcg tgacgatgtg gcaaaagttg ctgcctggtt
     5581 ccatcaacat ggcctgaacc gactggtatt gagcatgggc ggcgacggcg tttattacag
     5641 cgatatcagc ggtaaaaatg gctggtctgc gccgatcaaa accaatgtta ttaatgttac
     5701 cggagcgggc gatgccatga tggcgggact tgcttcgtgt tgggtagacg gaatgccgtt
     5761 tgccgaatct gttcgtttcg cacagggatg ttcgtcaatg gcgctctcct gtgaatacac
     5821 caataacccc gatttatcga ttgccaacgt tatatcgtta gtggagaacg cagaatgtct
     5881 gaattaaaaa tttcccctga attattacaa atttccccgg aagtgcagga cgctttaaaa
     5941 aacaaaaaac cggttgtggc gctggaatcg accattattt ctcacgggat gccgttccca
     6001 caaaatgccc agaccgcaat tgaagtagaa gaaactattc gtaaacaggg cgcagtacct
     6061 gccactatcg ccattattgg cggcgtgatg aaagtgggtt taagcaaaga aaaaattgaa
     6121 ttactgggtc gtgaagggca taacgtgacc aaagttagtc gtcgcgattt accttttgtt
     6181 gttgccgccg gaaaaaatgg cgcaaccact gtggcttcaa cgatgattat tgcggcgctt
     6241 gccggaatta aagtatttgc caccggggga attggtggtg tgcatcgcgg ggcggaacat
     6301 accttcgata tttctgccga tttgcaagaa ctggcaaata ctaatgtcac cgttgtttgt
     6361 gccggggcga aatctattct cgatttagga ttaaccactg agtatttaga aaccttcggt
     6421 gtgccgttaa ttggctatca gactaaagcg ctgcctgcgt ttttctgccg taccagctcg
     6481 tttgacgtca gcattcgtct cgacagcgcc agtgaaattg cccgtgcaat ggcggtgaaa
     6541 tggcaaagcg ggctgaacgg tggcctcgtg gtagcgaacc cgatcccgga acagtttgcg
     6601 atgccggagg aatctatcaa tgcagccata gatcaagccg tcgccgaagc cgaagagcag
     6661 ggcgttattg gtaaagaaag tacaccgttc ctgctggcgc gcgttgctga gctgaccggc
     6721 ggtgacagcc tgaaatccaa tatccagctg gtgttcaaca acgccattct ggcgagtgaa
     6781 attgccaaag aataccagcg tctcgcgggt taatttctga cgggcagaaa gccgtcgccc
     6841 gtactcatca agcataccaa aacctggcgc tgacgccggg tttactggca tgaagggaaa
     6901 atcattatgg atataatgag aagtgttgtg gggatggtgg tgttactggc aatagcattt
     6961 ctgttgtcag tgaataaaaa gagcatcagt ttgcgcacgg ttggagccgc actgctgctg
     7021 caaatcgcta ttggtggcat catgctctac ttcccaccgg gaaaatgggc agtagaacag
     7081 gcggcattag gcgttcataa agtgatgtct tacagtgatg ccggtagcgc cttcattttt
     7141 ggttcgctgg ttgggccgaa aatggatgtc ctgtttgacg gtgcgggttt tatcttcgcc
     7201 tttcgcgtac ttccggcgat tattttcgtt actgcgctca tcagtctgct gtactacatt
     7261 ggcgtgatgg ggctgctgat tcgcatcctt ggcagcattt tccagaaagc cctcaacatc
     7321 agcaaaatcg aatcttttgt tgcggttacc actattttcc tcgggcaaaa tgagatcccg
     7381 gcgatcgtta aaccgtttat cgatcgcatg aatcgcaacg agttgtttac cgcaatttgt
     7441 agcgggatgg cgtccattgc tggttcgatg atgattggtt atgccggaat gggcgtacca
     7501 attgactacc tgttagcggc atcgctgatg gcgatccccg gcggtatttt gtttgcacgt
     7561 attcttagcc cggcaaccga gccttcgcag gtcacatttg aaaatctgtc gttcagcgaa
     7621 acgccgccaa aaagctttat cgaagcggcg gcgagcggtg cgatgaccgg gctgaaaatc
     7681 gccgctggtg tagcgacggt ggtaatggcg tttgtcgcaa ttattgcgct gatcaacggc
     7741 attatcggcg gaattggcgg ctggtttggt ttcgccaatg cctctctgga aagtattttt
     7801 ggctatgtgc tggcaccgct ggcgtggatc atgggtgtgg actggagtga tgccaacctt
     7861 gctggtagcc tgattgggca gaaactggcg attaacgaat tcgtcgctta cctgagtttc
     7921 tccccatacc tgcaaacggg cggcacgctg gaagtgaaaa ccgtggcgat tatatccttt
     7981 gcgctttgtg gttttgctaa ctttggttct atcggtgttg tcgttggcgc attttcggct
     8041 attttgccaa aacgtgctcc agaaatcgcc cagcttggtt tacgggcgct ggcagcagca
     8101 acgctttcca acctgatgag tgcgactatt gccgggttct ttattggtct ggcgtaatgc
     8161 tcgatttgta gaccggataa aacgtattaa gcgttgcatc cggcaatcgc gctattgcaa
     8221 ataattttta ctgcatcatc ccggagaatt tattctccgg gtccatctcc agcgccagtc
     8281 ctgacaaatg ctttctgttt ttaattagat atcctttctt gcttttaatt aataaaccgt
     8341 cgtgaatgaa ttgcgcgaga acatataaaa gatgtcgata agaaacgcct aaatactctg
     8401 ccgcttgcgt atgcttttcg tgataaagat caccttcttg tgagagtaat ataaatgctg
     8461 ccaggcgatt cactaacgga aatgattgat tctgagttaa agaaacaata ttacgataat
     8521 ttttatgact taaggtgacg caaagttttc gtaaaaatag ggtgtcgttt aataacagcg
     8581 gacggtaatg tttcataggg agcgcaaggc accaacactc ttcaatagcc tgcaccgcac
     8641 gcggttcatg gtctttgtcg attaactcaa tctcgccaat aaaacaggga gcggcaaaga
     8701 aatcgatcag cgacacgcga ccattagcaa gcgtggcgta aagcctggcg cggcctcgcg
     8761 tcagataaaa cagccatgac ggctgttgcc cttcctgcac aatgtagtca cgcgctaaaa
     8821 aatgaaacag ccgcgtatcg gctgaaacat ccgttgaaaa gcaatcctta aacgcagatt
     8881 cactcatcaa ttgctgctta aggtcattat tgtggatttc tttcacgatg tttatccgct
     8941 atgagatttc tcatatttag catacgtacg ctgttgttaa gattcaatca tcggcccggt
     9001 cgaattttct ttgctgtaga aaaaaataac gcaactggaa acagaggaaa taaacaatgg
     9061 aaaagagaaa aattattctg gattgtgatc cgggtcatga tgatgctatt gcaatgatga
     9121 tggcagcgaa acatccggca atagatttat taggcatcac tattgtagtg ggtaatcaga
     9181 cgcttgataa aacattaatt aatggcctga atgtttgcca gaaactggag attaatgttc
     9241 cggtttatgc ggggatgccg caacccatta tgcgtaaaca aatcgttgcc gataatattc
     9301 acggtgaaac cggactggat ggcccggtat tcgagccgct gacccgccag gcagaaagta
     9361 cccatgcggt gaaatatatc atcgataccc tgatggcaag cgatggcgat atcactctgg
     9421 tgccggttgg tccgctttca aatatcgcgg tggcaatgcg tatgcaaccc gcaatcctgc
     9481 ccaaaatccg tgaaattgtg ctaatgggcg gcgcttacgg tacaggcaac ttcaccccat
     9541 ctgccgagtt caatatcttt gccgacccgg aagccgcccg cgtggtgttc acctccggcg
     9601 ttccattagt gatgatgggc ctcgatctta ccaaccagac cgtttgcacc ccggatgtaa
     9661 ttgctcggat ggaaagggta ggcggccctg ccggagagct gttcagcgac atcatgaact
     9721 tcactctcaa aacgcagttc gaatactacg gcctggctgg cggcccggtg catgacgcca
     9781 cctgcattgg ttatctgatc aaccctgatg gcattaaaac ccaggatatg tacgtcgaag
     9841 tggacgtcaa cagcggccct tgctacgggc gtacggtctg cgacgaactg ggcgttcttg
     9901 gcaagcccgc taataccaaa gtcggcatca ctattgatac cgactggttc tggggcttag
     9961 tcgaagagtg cgtgcgcggc tacatcaaaa cccattaagt gttgataaca agccgggaca
    10021 gtatcgcaag atgcaggccc ggctatttct gatgggcaga aagccgtcgc ccaaaatgac
    10081 atgaagagat aatcactatg gatgtcatga gaagtgttct gggaatggtg gtactgctgg
    10141 caattgcgtt tttactgtca gtaaacaaga agaagatcag cctgcgtacc gttggcgcgg
    10201 cgttagtgtt acaggtcgtg attggcggga ttatgctttg gttaccgcca gggcgttggg
    10261 tcgcagaaaa agtcgctttt ggcgtccaca aagtgatggc gtacagcgac gcaggtagcg
    10321 catttatctt cggttctctg gtcggaccga aaatggatac cttatttgat ggcgcaggat
    10381 ttatctttgg tttcagggta ttaccggcaa ttatcttcgt cactgcactg gtgagtattc
    10441 tctactacat cggtgtgatg gggatcttaa ttcgcattct cggcggtatc ttccagaaag
    10501 ctttaaatat cagcaagatc gagtcattcg tcgcggtcac caccattttc ctcgggcaaa
    10561 acgaaattcc ggcgatcgtc aaacccttta tcgatcgtct gaatcgcaat gaattattta
    10621 cagcgatttg tagtggcatg gcctcgattg ctggttcgac aatgattggt tacgccgcac
    10681 tgggcgtacc tgtggaatat ttgctggcgg catcgttaat ggcgatccct ggcgggatct
    10741 tgtttgcccg cctgttaagc ccggctacgg aatcttcgca ggtttctttt aataacctct
    10801 ctttcaccga aacaccgcca aaaagcatta ttgaagccgc tgcgacaggg gcaatgaccg
    10861 ggctgaaaat cgccgccggt gtagcgacag ttgttatggc atttgtcgcc atcattgcgt
    10921 taattaacgg tattatcggc ggcgttggcg gctggtttgg ttttgaacat gcctcgctgg
    10981 agtccatttt aggttacctg ctggccccac ttgcgtgggt aatgggtgtt gactggagtg
    11041 atgcaaatct tgccgggagt ttgattggac agaaactggc aataaatgaa tttgtcgctt
    11101 atctcaattt ctcaccctat ctgcaaacgg ctggcactct ggatgctaaa accgtggcga
    11161 ttatttcctt cgcgttgtgc ggtttcgcta actttggttc tatcggggtg gtggtggggg
    11221 cgttttctgc ggttgcgcca caccgtgcgc cggaaatcgc ccagcttggt ttacgcgcgc
    11281 tggcggcggc gacactttct aatctgatga gtgccaccat tgccgggttc tttattggtt
    11341 tagcgtagtg tgcaatttat gagccggatt agatgcggtc aagcgtcgaa tccggcaaca
    11401 ttgttacacc attggcacta atgacagcgc gttatcggca gacagggtag ggttgtttaa
    11461 gctgccgctg gcgcgtgaaa ttgccgcgca ggccatcgca aaacgggcgc tgtcacggaa
    11521 attgtttccc tccagaaagc tatataccag acccgccata aaaccatcgt cagcaccaaa
    11581 actgtctacc gtcgtatgcg ctggcacagt cagcaaaaat tgttcaccat ccttttcgct
    11641 gaaataaact gactcatcgg gcaaataaac aaacagttgc tgaacacctt gctgatgcaa
    11701 tgcattcact gcggcattac ggtcagcatc gctggtgatc gcctgtcccc ataaaatttc
    11761 cagctccggt aaagtgggtt tcagggtgtg aatatgcgcc agccagtgtt tgattttgcc
    11821 cgctttgaat tctgaaacgg tatcgacaaa caccgggatc tcatcagcga gggtaaagac
    11881 ccattccagc gcctcggctg tcaggttgca atcagccagt accacgcccg catgacgaag
    11941 taaatcgcgc gacccgttca gtagttgcgg tgtcaactgt tccagcagat gggtatcgtt
    12001 aatcgccagc acggtttgat cgtctcgatt ggcaattgcc agatacgtcg atgtgctttg
    12061 accatgcaaa cgaacgcagc cggagacatt cacgccggca cggcgcgttt cttctaggag
    12121 catttcgcca taaaagtcat cgccaatcac tgaaagcaaa tggacgtcac ggcgctaaca
    12181 gcgccagatt gtgggcgata ttgcgtccaa cgccgcctgc cgagcaatga attgtaccgg
    12241 gatgagaagc cgcttgcggg taatggatat ccgccatccc gcgaatatcc atattgattg
    12301 tccccaccac tacgcagtat tcctgctcgg tgagaatgta acctttgcct ttaatccggc
    12361 ctttgcgcat taaatccata atatgcgccg caacgcgcga acgactgatt tgcagcatgt
    12421 ccgcaatttc gttctgctga atcagcgggt tacgccgtaa aattgcaagg atctcctttt
    12481 cccggttatt catcttcagg ctaccgcttt ttcagtttgt tgcgctttca gccaggcgat
    12541 ctcttctgcc cagatatccg ggttgatggt ttcaaggatc agcggaatgc cgtcgaaacg
    12601 gtcgttctgc ataatccagc ggaacgcatc atgaccgata ttgccttcac cgaggctatg
    12661 atggcggtca acgcggctgc caaaggtact tttcgcatcg ttaaggtgca tcccgcgcag
    12721 atacttaaag ccgacaatac gggcaaaatc agcgaacgtt ttttcgcatt cggctggagt
    12781 acgcaaatca tacccggcag cgaaagcatg gcaggtatca atgcagacgc cgacgcggga
    12841 tttatcttcc acgccgtcga taatcgccgc gagatgttcg aatttaaacc ctaagttact
    12901 gccctgaccg gcggtgtttt caatcaccgc tgttacacct tgagttttat ccagcgcaat
    12961 gttgatggat tcggcaatac gcgcaaggca atcctcttct gaaatctgca tcagatggct
    13021 gccagggtgg aagttgagca aagaaagccc cagttgttcg caacgctgca tttcatctat
    13081 aaaggcatcg cgcgattttt ccagagcttc agtgaccgga tgtccgaggt tgatcagata
    13141 actgtcgtgg ggaagaattt gcgccgatgt gtagtgatat ttttcacagg cggctttgaa
    13201 ttcatcgatg gtttgcgtcg tgagcggtgc ggcacgccac tgacgttggt ttttggtgaa
    13261 caaggcaaac gcggttgcgt cgatttcggc ggcgcgaatt gcggcatttg ccagaccgcc
    13321 agcagcacta acgtgcgctc caatgtattt catgcgagga ctcctgttaa acccgctgga
    13381 ggaaaacggt aatgatagcg ggttaacagg agcaagaagt agtggtttat gcgatgacgc
    13441 tttgaatcac atagttaatc gcaccaccac caacaatcag ccaggcaaac agtaccagtg
    13501 ccatcagcag aggtttcgcc ccagcttttt tcagcgtgct gacgtgagtg gtcagaccca
    13561 gcgccgccat cgccattgcc agcaggaagg tatccagcgt taccagcatg ttcaccacgc
    13621 tctgcggtaa caggtggaac gagttaaaga tggcaactac gatgaacaag atggcaaacc
    13681 acggaatagt gattttgctt ttctcgccgc tgttcgcccc agacagctgt ttaacacgcg
    13741 ccgccagcag gatgaggaac ggagccagca tcatcacgcg cagcattttg gaaataactg
    13801 ctgcgttttc tgcatccggg ctgatggcat gacctgccgc caccacctgc gccacttcgt
    13861 gcacagtaga accaatgtag ataccgaaag tttccggact aaaccattga gacatcagcg
    13921 gatatatcgc cgggtagagg aaaatcgcga cggtcccgaa gataacaacg gttgcaacag
    13981 ccacggttac tttactggct tccgctttca ctaccggctc agtcgccagt accgcggcag
    14041 caccacagat actgctaccg gcaccgatca accaactggt gtgcttatcc agaccaaaca
    14101 ctttctgccc caggaagcaa gccagcagga aggtactgga cagcgtcagc acgtcaatga
    14161 tgatcccact gataccgaca tcggcaattt gcgagaacgt cagacggaag ccataaagaa
    14221 tgatacccag acgtaataaa tattgcttgg caaacagcac accaccgtca cagcttttcc
    14281 agatgtgcgg atagatggtg ttgcctaaaa ccatccccaa caagattgcg agggtgaggg
    14341 cactaaaccc ggcacccgca accgcgggaa tggaaccacc ccacagggcg accccggtga
    14401 taactgcact cagggctaac cccggaataa aatgccacag tgtacgatgt tgtttctgta
    14461 aggtgatatt cgtcataacc ctctccttta accgggcata aggttacgac taactggttt
    14521 aaaaataaaa ttgattatat atttataatt aatctttata agtggtaagc gactatgcac
    14581 atcaccctcc ggcagttgga agtttttgca gaagtattga aaagtggatc aaccacccag
    14641 gcgtcggtga tgctggcgtt gtcgcaatca gcagtgagtg cagccttgac tgacctggaa
    14701 gggcagcttg gcgttcaact gtttgatcgc gtggggaaaa gactggttgt taatgaacac
    14761 gggtggctgc tctatccgcg agcgttggca ctgcttgaac aggcggttga aatcgaacaa
    14821 ctgtttcgcg aagacaacgg cgcgattcgt atctatgcca gtagtaccat cggtaactac
    14881 attctgcctg cagttatcgc ccgttatcgc catgattatc cgcagttgcc gattgaactt
    14941 agcgttggga acagccagga cgtgatgcaa gcggtgctgg atttccgcgt tgatattggc
    15001 tttattgaag gaccgtgcca cagcactgaa atcatttctg aaccgtggct ggaagacgag
    15061 ctggtggttt tcgccgcacc gacttcgccg ttggctcgtg gtccggtcac cttagaacag
    15121 ctggccgctg cgccgtggat cctgcgtgaa cgcggttccg gcacgcggga gattgtcgat
    15181 tatctgttgc tgtcacattt accgaagttt gagatggcga tggaattagg taactccgag
    15241 gcaatcaaac atgcggtgcg tcatgggttg ggaattagtt gcctgtcgcg acgtgtgatt
    15301 gaagatcaat tgcaggcagg cacattaagt gaagttgcgg tccctctgcc gcgcctgatg
    15361 cgtacgttgt ggcgtataca tcatcggcaa aaacaccttt ccaacgcgct acggcgcttt
    15421 ctggactatt gcgatcccgc aaatgtgccg cgttaagttg ctgtacaaga acatgctggt
    15481 gctgtgtcga tttcgtgacg cagcgccttc agcatgcatt cgccagaaaa gagattggct
    15541 gctttactta taatccctgg gcgatcatga aggtgtctta taaccgtgta tttctgccgg
    15601 aaggattgcc aatcgtctgc tacaatcgcg ccatcatttt taagatggat agcatttttg
    15661 tatgggttcc gaaactaaaa ctacagaagc gccgggctta cgccgtgaat taaaggcgcg
    15721 tcacctgacg atgattgcca ttggcggttc catcggtaca ggtctttttg ttgcctctgg
    15781 cgcaacgatt tctcaggcag gtccgggcgg ggcattgctc tcgtatatgc tgattggact
    15841 gatggtttac ttcctgatga ccagtctcgg tgaattggct gcatatatgc cggtttccgg
    15901 ttcgtttgcc acttacggtc agaactatgt tgaagaaggc tttggcttcg cgctgggctg
    15961 gaactactgg tacaactggg cggtgactat tgccgttgac ctggttgcag cacagctggt
    16021 catgagctgg tggttcccgg atacaccggg ctggatctgg agtgcgttgt tcctcggcgt
    16081 tatcttcctg ttgaactaca tctctgttcg cggctttggt gaagcggaat actggttctc
    16141 actgatcaaa gtcacgacag ttattgtctt tatcatcgtt ggcgtgctga tgattatcgg
    16201 tatcttcaaa ggcgcgcagc ctgcgggctg gagcaactgg acaatcggcg aagcgccgtt
    16261 tgctggtggt tttgcggcga tgatcggcgt agctatgatt gtcggcttct ctttccaggg
    16321 aaccgagctg atcggtattg ctgcaggcga gtccgaagat ccggcgaaaa acattccacg
    16381 cgcggtacgt caggtattct ggcgaatcct gttgttctat gtgttcgcga tcctgattat
    16441 cagcctgatt attccgtata ccgatccgag cctgctgcgt aacgatgtta aagacatcag
    16501 cgtcagtccg ttcaccctgg tgttccagca cgcgggtctg ctctctgcgg cggcggtgat
    16561 gaacgcagtt attctgacgg cggtgctgtc agcaggtaac tccggtatgt atgcgtctac
    16621 tcgtatgctg tacaccctgg cgtgtgacgg taaagcgccg cgcattttcg ctaaattgtc
    16681 gcgtggtggc gtgccgcgta atgcgctgta tgcgacgacg gtgattgccg gtttgtgctt
    16741 cctgacctcc atgtttggca accagacggt atacctgtgg ctgctgaaca cctccgggat
    16801 gacgggtttt atcgcctggc tggggattgc cattagccac tatcgcttcc gtcgcggtta
    16861 cgtattgcag ggacacgaca ttaacgatct gccgtaccgt tcaggtttct tcccactggg
    16921 gccgatcttc gcattcattc tgtgtctgat tatcactttg ggccagaact acgaagcgtt
    16981 cctgaaagat accattgact ggggcggcgt agcggcaacg tatattggta tcccgctgtt
    17041 cctgattatt tggttcggct acaagctgat taaaggaact cacttcgtac gctacagcga
    17101 aatgaagttc ccgcagaacg ataagaaata agtttcctcc cttccttgct aagccctctc
    17161 aaccgagagg gctttttcaa ttccatttcc ctgacaaatc atgcggatat aaaatttaac
    17221 atttggattg ataattgtta tcgtttgcat tatcgttacg acgcaatcaa aaaaggctga
    17281 caaatcagag gctgttccgg ctttctggga tgatcacctg catacaaata agtccaccgt
    17341 gatgctgccg tacgcaaggg gacgtaaaga agatgtgagc gatacccatt ttattttcgt
    17401 agttacctca tggagatatg gaatgtttag gttgaaccct ttcgtacggg tcgggctgtg
    17461 tttgtccgct atttcttgtg catggcctgt gttagcggtc gatgatgatg gcgaaacgat
    17521 ggttgtcact gcatcttccg tggaacaaaa tcttaaagat gcacccgcca gtatcagcgt
    17581 cattacccag gaagacctgc agcgaaaacc ggtacagaat ctgaaggatg tcctcaaaga
    17641 agtgcctggc gtacaactga cgaacgaagg ggataaccgt aagggcgtta gtattcgtgg
    17701 tctggacagc agctacaccc tgattcttgt cgacggtaaa cgcgttaact cccgcaatgc
    17761 cgtcttccgc cacaatgatt tcgatctgaa ctggatcccg gtcgattcca tcgaacgtat
    17821 tgaagtggtt cgtggcccga tgtcgtcgct atacggctcc gatgcgctcg gcggtgtagt
    17881 gaatatcatc accaaaaaaa tcggtcagaa atggtcgggt accgttaccg tcgataccac
    17941 cattcaggaa catcgcgatc gcggtgatac ctataacggt cagttcttta ccagtggacc
    18001 attaattgat ggtgtgctgg gaataaaagc ttacggcagc ctggcaaaac gtgaaaagga
    18061 tgacccgcaa aactcaacga ccaccgatac cggagaaacg ccgcgtattg aaggattctc
    18121 cagccgcgac ggcaatgtcg aatttgcctg gacaccgaat caaaatcacg attttactgc
    18181 cggatacggt ttcgaccgtc aggatcgtga ttccgactcg ctggacaaaa accgcctgga
    18241 acgccagaac tactccgtca gccataatgg gcgttgggat tacggcacca gcgaactgaa
    18301 atactacggt gagaaagtcg agaacaaaaa ccctggcaac agcagcccga taacttccga
    18361 aagcaatacg gtcgacggca aatacacgtt gccgctgacg gcgattaatc agtttctcac
    18421 ggttggcggt gaatggcgtc acgacaaact tagcgatgcg gtgaacctga ccgggggaac
    18481 cagctccaaa acgtctgcca gccagtacgc gctgtttgtg gaagatgaat ggcggatctt
    18541 cgagccgctg gcgctgacga ccggcgtgcg tatggacgat cacgaaacct acggtgaaca
    18601 ctggagtccg cgtgcctacc tggtttataa cgccaccgac accgtaacgg tgaaaggggg
    18661 ctgggcgacg gcatttaaag caccttctct gttgcaactt agccctgact ggacgagcaa
    18721 ttcctgccgt ggcgcatgta agattgtggg tagcccggat ctgaaaccag aaaccagcga
    18781 aagttgggag ctggggcttt actacatggg tgaagaaggc tggctggaag gggttgaatc
    18841 cagcgttatc gttttccgta acgatgtgaa agatcgtatc agcatcagcc gtacgtctga
    18901 cgtcaacgct gcaccgggct accaaaactt tgttggtttt gagacgggcg ctaacggacg
    18961 gcgcataccg gtatttagct actac
//
LOCUS       DADLSB010000073        18938 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.073, whole genome shotgun sequence.
ACCESSION   DADLSB010000073 DADLSB010000000
VERSION     DADLSB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.073"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            426..1007
                     /locus_tag="JN331_002392"
     CDS             426..1007
                     /locus_tag="JN331_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7260520.1"
                     /translation="MKQQEEHNNKIVLLEKQQAQLKSQLETIQKQQTGIINSTKNLTH
                     VIKSVKDQQNTFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLL
                     STPISNIGFNATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVS
                     PNNLGYLKLAINMENIQFDNLPSAENRQKRSKK"
     gene            1051..5713
                     /locus_tag="JN331_002393"
                     /pseudo
     CDS             1051..5713
                     /locus_tag="JN331_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            6081..7736
                     /gene="lldP"
                     /locus_tag="JN331_002394"
     CDS             6081..7736
                     /gene="lldP"
                     /locus_tag="JN331_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY7260521.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7736..8512
                     /gene="lldR"
                     /locus_tag="JN331_002395"
     CDS             7736..8512
                     /gene="lldR"
                     /locus_tag="JN331_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY7260522.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8509..9699
                     /gene="lldD"
                     /locus_tag="JN331_002396"
     CDS             8509..9699
                     /gene="lldD"
                     /locus_tag="JN331_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY7260523.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9895..10368
                     /gene="trmL"
                     /locus_tag="JN331_002397"
     CDS             9895..10368
                     /gene="trmL"
                     /locus_tag="JN331_002397"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY7260524.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10421..11242)
                     /gene="cysE"
                     /locus_tag="JN331_002398"
     CDS             complement(10421..11242)
                     /gene="cysE"
                     /locus_tag="JN331_002398"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY7260525.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAISIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11322..12341)
                     /gene="gpsA"
                     /locus_tag="JN331_002399"
     CDS             complement(11322..12341)
                     /gene="gpsA"
                     /locus_tag="JN331_002399"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY7260526.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12341..12808)
                     /gene="secB"
                     /locus_tag="JN331_002400"
     CDS             complement(12341..12808)
                     /gene="secB"
                     /locus_tag="JN331_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY7260527.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12871..13122)
                     /gene="grxC"
                     /locus_tag="JN331_002401"
     CDS             complement(12871..13122)
                     /gene="grxC"
                     /locus_tag="JN331_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY7260528.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13264..13695)
                     /locus_tag="JN331_002402"
     CDS             complement(13264..13695)
                     /locus_tag="JN331_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY7260529.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13940..15484
                     /gene="gpmM"
                     /locus_tag="JN331_002403"
                     /gene_synonym="pgmI"
     CDS             13940..15484
                     /gene="gpmM"
                     /locus_tag="JN331_002403"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY7260530.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15494..16777
                     /gene="envC"
                     /locus_tag="JN331_002404"
     CDS             15494..16777
                     /gene="envC"
                     /locus_tag="JN331_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY7260531.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16781..17740
                     /locus_tag="JN331_002405"
     CDS             16781..17740
                     /locus_tag="JN331_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY7260532.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17727..18761)
                     /gene="waaH"
                     /locus_tag="JN331_002406"
     CDS             complement(17727..18761)
                     /gene="waaH"
                     /locus_tag="JN331_002406"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAY7260533.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPIDRLRSTGVLTGPDWLRMGLFSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
BASE COUNT         4796 a         4867 c         4874 g         4401 t
ORIGIN      
        1 tacgtctctt tttttcggat ttaattgtga aattttaatc atcatattca caaaatcaat
       61 tatctgtaaa atttttccca gacatggttt ctttcatatt tgacatttat taataatcat
      121 cattagaatc cacgaaaaat aatatattca tacaaacaca gacatctttt gaaacgaatg
      181 atacattttt atgatgaatg cttaaaacaa tatttaatcg acacacatca ctgtcattaa
      241 tgtaaagcaa catagctcac caataataca atggacaact ctaccggcat tgatcgctgg
      301 tcggaggaaa taaggatttt tacatgaact tgaagaagat ttttttcagt gctgtcactg
      361 tttcagttct ctgcgcatta acgggatgtg actacattga agaaggcaag ccagaaagta
      421 gtttactgaa acaacaagaa gaacataaca ataaaattgt gctacttgaa aaacaacaag
      481 cacagctaaa gagtcaactc gaaactattc aaaaacaaca aactggcata ataaacagta
      541 caaagaattt aactcacgta attaaatccg ttaaagacca acaaaatact tttattttta
      601 ccgagtttaa cccagcaaaa acgaagtatt tcatcctgaa caacggttcg gtggctttag
      661 cgggtcgagt gttatccatt gacgccacag agaatggtag tgttattcat atctcactgg
      721 tcaacttatt aagtacacct atctcaaaca ttggttttaa tgcgacatgg ggtggtgaaa
      781 aacctgtcga tgccaaagag tttgcccggt ggcaacaatt acttttcaac acatcaatga
      841 aatccacatt gaaattatta ccaggtcaat ggcaagacat taatttgacc ctgaagggtg
      901 tatcgcccaa taacctagga tatctgaagt tagccatcaa catggaaaat attcagttcg
      961 acaatcttcc ctctgctgaa aatcggcaga aaagaagcaa aaaataaatg cagttagtac
     1021 gcttataaca aggaatagcg agcatcatta atgaacaaaa tatttaaagt tatctggaac
     1081 cctgcgacag ggaattatac tgttaccagc gaaacggcaa aaagccgtgg caagaaatct
     1141 gggcgcagta agctgttaat ttctgcgctg gttgcgggtg gaatgttgtc gtcgtttggg
     1201 gcattggcga atgccgggaa tgacaacggt cagggtgttg attacggtag tggatcagct
     1261 ggcgacggct gggttgctat aggcaaaggg gcgaaagcaa atacttttat gaacaccagt
     1321 ggttccagta ctgctgtggg ttatgacgct atagctgaag gccaatatag ctctgccatc
     1381 gggtcaaaaa cccatgcgat tggtggtgca tcaatggcct ttggggttag tgcaatatca
     1441 gaaggcgata gaagtatagc actgggtgcc tcttcgtatt cattgggcca atactcaatg
     1501 gccctcggcc gttattcaaa agcattgggt aaattgtcta ttgctatggg ggactcttcc
     1561 aaagcggaag gagcaaacgc cattgccctg gggcgtagca gtgtagctag cggtactgac
     1621 agcctcgcat ttggcagaca atcacttgcc agcgcagcga acgctattgc gataggtgct
     1681 gagaccgaag ccgctgaaaa tgcaactgct attggcaata atgcgaaggc aaaagggact
     1741 aatagcatgg caatggggtt cggaagcctt gccgataaag tcaatactat cgcattagga
     1801 aatggcagcc aggctctggc agataatgca atcgccatag gccagggcaa caaagctgat
     1861 ggcgtggatg ccatcgctct gggtaatggt agccagtcga gaggcttaaa caccattgcc
     1921 ttaggcacag ccagtaatgc aactggtgat aagagtcttg cgcttggtag taatagcagt
     1981 gccaacggta ttaactctgt cgcgctgggc gcagattcca ttgcggattt agacaatacc
     2041 gtctctgtcg gcaatagttc attaaaacgc aagatcgtta atgtgaaaaa tggcgcgatc
     2101 aagtctgaca gttacgatgc cattaatggt tcacagcttt atgccattag cgactcggta
     2161 gcaaaaaggc ttggaggagg ggctgcagta gatgttgatg acggtactgt tacagcacca
     2221 acctacaatt taaaaaatgg tagcaaaaat aacgtagggg ctgcgctcgc tgtacttgat
     2281 gaaaacaccc tgcaatggga ccaaaccaaa ggcaaataca gcgctgctca tggtactagt
     2341 agcccaactg ccagcgtaat caccgatgtt gcggatggca cgatttcagc ctccagtaag
     2401 gatgcggtta acggttccca actgaaagct accaatgacg atgtcgaagc caacaccacc
     2461 aatatcacca acctgacgga ttccgttggt gaccttcagg ctgatgccct gctctggaac
     2521 gaaactaaaa aggcattcag tgcagctcac gaccaggata ccaccagcaa aatcaccaac
     2581 gttaaagatg ccgacctgac ggctgacagc actgatgctg ttaacggctc tcagctgaaa
     2641 accaccaacg atgctgtggc gacgaatacc accaatatcg ccaataacac ttccaatatt
     2701 gccactaaca ccaccaacat ctctaacctg actgagacgg tgactaatct tggtgaggat
     2761 gcgctgaaat gggataagga caatggtgta ttcacggcag ctcatggcac cgagaccacc
     2821 agcaaaataa ccaacgttaa agatggcgac ctgacgactg gcagcaccga tgccgttaac
     2881 ggctctcagc tgaaaaccac caacgatgcc gtggcgacga ataccaccaa tatcgccact
     2941 aacaccacca acatctctaa tctgactgag acggtgacta atcttggtga ggatgcgctg
     3001 aaatgggata aggacaatgg tgtcttcact gcagctcatg gcaacaatac cgccagcaaa
     3061 atcaccaata tcctggacgg cacagtcact gcaaccagtt ccgatgccat taacggtagc
     3121 cagctttatg acttaagcag caatatcgcc acctacttcg gcggcaatgc ttctgtgaat
     3181 actgacggtg tgtttaccgg tccaacctac aaaatcggtg aaacaaatta ttataacgtc
     3241 ggcgatgcac tggctgcgat taactcctca tttagcacgt ctctcggcga tgctctgctt
     3301 tgggatgcca ccgcaggtaa attcagtgcc aaacacggta ctaatggtga cgcaagcgtg
     3361 atcactgatg tcgcagatgg tgaaatttca gactccagtt ctgacgcagt aaacggctca
     3421 caactccacg gcgtgagcag ttatgttgtt gatgcgctgg ggggggggtg ccgaagtcaa
     3481 tgcagacggc accatcactg cgccgacgta caccattgct aatgctgatt acgataatgt
     3541 cggtgatgcc ctgaatgcta tcgataccac tcctgacgac gctctgctct gggatgcgga
     3601 cgccggtgaa aatggtgcat ttagcgccgc tcacggaaaa gataaaactg ccagtgtaat
     3661 cactaacgtc gctaacggtg caatctctgc tgccagcagc gacgcgatta acggctcaca
     3721 actctatacc accaataagt acatcgctga tgcgctgggt ggtgacgcag aagtcaacgc
     3781 tgacggcacc atcaccgcac cgacttacac cattgcgaac gccgagtaca acaacgtcgg
     3841 tgacgccctg gatgcgcttg atgataacgc cctgctgtgg gatgagactg ccaatggcgg
     3901 tgctggagcc tacaatgcca gccatgacgg taaagccagc atcatcacta atgtcgctaa
     3961 tggcagtatt agtgaggaca gtaccgatgc agtgaacggt tctcagttga atgcgacgaa
     4021 tatgatgatt gagcagaaca cccaaattat caatcagctc gctggtaaca ccgacgcaac
     4081 ctatatcgaa gaaaatggcg ctggtatcaa ctacgttcgt actaacgaca acgatttagc
     4141 ctttaacgat gcaagcgcct ctggtgtcgg cgctacagct gtaggttata acgctgtcgc
     4201 gtctggtgcc agcagcgtag ccattggtca gaacagcagc agcaccgttg ataccggtat
     4261 tgcgctgggt agcagctccg tttccagccg tgtgattgcc aaaggttctc gtgacactag
     4321 cgtaacggaa aatggcgtgg tgattggtta tgacaccact gacggcgaac tgctaggtgc
     4381 attgtcaatt ggtgatgacg gtaaataccg ccaaatcatc aacgtagctg atggttcaga
     4441 agcccatgac gccgttacgg ttcgccagtt gcagaacgct attggagcgg tcgccactac
     4501 gccaaccaag tactttcacg ccaactcaac ggcagaagac tcactggccg ttggtgaaga
     4561 ctcgctggca atgggtgcga aaactgtcgt taatggtaat gcaggtattg gtatcggttt
     4621 gaacactctg gttctggctg atgcgatcaa cggcattgct atcggcagta acgcacgcgc
     4681 aaaccatgca aacagtatcg caatgggtaa cggttctcag accacccgtg gtgcacagac
     4741 tggctacacc gcctacaaca tggacgcacc gcagaactct gtaggtgagt tttctgtcgg
     4801 tagtgaagac ggtcaacgtc agatcacaaa cgtcgcagct ggttcagcgg ataccgatgc
     4861 ggttaacgtg ggtcagttga aagtcactga tgagcgcgta gcgcaaaata cccagagcat
     4921 tactaacctg aacaatcagg tcactaatct ggatactcgc gttactaata tcgaaaacgg
     4981 tattggcgac attgtcacca ccggtagcac caagtacttc aagaccaaca ccgatggcgt
     5041 agatgccaac gcccagggta aagatagcgt tgctattggt tctggttcca ttgctgccgc
     5101 tgacaacagc gtcgcactgg gtaccggttc cgttgcagag gaagaaaata caatctctgt
     5161 aggttcttct actaaccaac gccgtatcac caacgtagct gccagtgtta atgccaccga
     5221 tgcggttaac gtgtcgcagc tgaaatcttc tgaagcaggc ggcgtgcgct acgacaccaa
     5281 agctgatggt tctatcgact atagcaatat caccctcggt ggtggcaacg gcagtacgac
     5341 tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac gcggtgaact acgcgcagtt
     5401 gaagcaaagc gcgcaggaaa cgaagcaata caccgatcag cggatggttg agatggataa
     5461 caaactgtct aaaactgaaa gcaagttgag cggtggtatc gcttctgcaa tggcaatgac
     5521 cggtctgccg caggcttata caccgggtgc cagcatggct tctattggtg gcggtactta
     5581 caacggtgaa tcggcagttg ctttaggtgt atcgatggtg agcgccaatg gtcgttgggt
     5641 ctacaaatta caaggtagta ccaatagcca gggtgaatac tccgccgcac tcggtgccgg
     5701 tattcagtgg taatgatcca ttaagttagt gtgactaagg gcaaacgcca aattgcctga
     5761 tgcgttacgc gcatcaggca agataaggcg ctccgggtca acaatctaag ggcaattctc
     5821 tgatgaggat tgcccttttc tttatcagac atctcccccc acaagaattg gccctaccaa
     5881 ttcttcgctt atctgacctc tggttcacaa ttccccaatt aaaactcaca tcaatattgc
     5941 caacacataa catttagtta accattcatt gtcattatcc ctacacaaca caattggcag
     6001 cgccactttt acacaacgtg tgacaaggag atgagcaaca gactcattac acgatgtgcg
     6061 tggactctag gagacctgca atgaatctct ggcaacaaaa ctacgatccc gccgggaata
     6121 tctggctttc cagtctgata gcatcgcttc ccatcctgtt tttcttcttt gcgctgatta
     6181 agctcaaact gaaaggatac gtcgccgcct catggacggt agcaatcgcc cttgccgtgg
     6241 ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc ctcggtggtt tatggcttct
     6301 tctatggttt gtggcccatc gcgtggatca ttattgcagc agtgttcgtc tataagatct
     6361 cggtgaaaac cgggcagttt gacatcattc gctcgtctat tctttcgata acccctgacc
     6421 agcgtctgca aatgctgatc gtcggtttct gtttcggcgc tttccttgaa ggagccgcag
     6481 gctttggcgc accggtagca attaccgccg cattgctggt cggcctgggt tttaaaccgc
     6541 tgtacgccgc cggactgtgc ctgattgtta acaccgcgcc agtggcattt ggcgcgatgg
     6601 gcattccaat cctggttgcc ggacaagtaa caggcatcga cagctttgag attggccaga
     6661 tggtggggcg tcagctaccg tttatgacca ttatcgtgct gttctggatc atggcgatta
     6721 tggacggctg gcgcggtatc aaagagacgt ggcctgcggt cgtggttgca ggcggctcgt
     6781 ttgccatcgc tcagtacctt agctctaact tcattgggcc ggagctgccg gacattatct
     6841 cttcgctggt atcactgctc tgcctgacgc tgttcctcaa acgctggcaa ccagttcgcg
     6901 tattccgctt cggtgacttg ggggcgtcac aggttgatat gacgctggcc cacaccggtt
     6961 acaccgcggg tcaggtgtta cgtgcctgga caccgttcct gttcctgacc gctaccgtaa
     7021 cactgtggag catcccgccg tttaaagccc tgttcgcatc gggtggtgcg ctgtatgagt
     7081 gggtgatcaa cattccggtg ccgtacctcg ataaactggt tgcccgtatg ccaccagtgg
     7141 tcagcgaggc tacagcctat gccgccgtgt ttaagtttga ctggttctct gccaccggca
     7201 ccgccattct gtttgctgca ctgctctcga ttgtctggct gaagatgaaa ccgtctgacg
     7261 ctatcagcac cttcggcagc acgctgaaag aactggctct acccatctac tccatcggca
     7321 tggtgctggc gttcgccttt atctcgaact attccggact gtcatcaaca ctggcactgg
     7381 cactggcgca caccggtcat gcattcacct tcttctcgcc gttcctcggc tggctggggg
     7441 tattcctgac cgggtcggat acctcatcta acgccctgtt cgccgcgctg caagccaccg
     7501 cagcacaaca aattggcgtc tctgatctgt tgctggttgc cgccaatacc accggtggcg
     7561 tcaccggtaa gatgatctcc ccgcaatcta tcgctatcgc ctgtgcggcg gtaggcctgg
     7621 tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca cagcctgatc ttcacctgta
     7681 tggtgggcgt gatcaccacg cttcaggctt atgtcttaac gtggatgatt ccttaatgat
     7741 tgttttaccc agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc tgattgatga
     7801 aaaaaacctg gaagcgggca tgaagttgcc cgctgagcgc caactggcga tgcaactcgg
     7861 cgtatcacgt aattcactac gcgaggcgct ggcaaaactg gtgagcgaag gcgtgctgct
     7921 cagtcgacgc ggcggcggga cgtttattcg ctggcgtcat gacacatggt cggagcaaaa
     7981 catcgtccag ccgctaaaaa cgctgatggc cgatgatccg gattacagtt tcgatattct
     8041 ggaagcccgc tacgccattg aagccagcac cgcatggcat gcggcaatgc gcgccacacc
     8101 tggcgacaaa gaaaagattc agctttgctt tgaagcaacg ctaagtgaag acccggatat
     8161 cgcctcacaa gcggacgttc gttttcatct ggcgattgcc gaagcctcac ataacatcgt
     8221 gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa tcctcagtga agcatagccg
     8281 tcagcggatg tatctggtgc caccggtttt ttcacaactg accgaacaac atcaggctgt
     8341 cattgacgcc atttttgctg gtgatgccga cggggcgcgt aaagcaatga tggcgcacct
     8401 tagttttgtt cacaccacca tgaaacgatt cgatgaagat caggctcgcc acgcacggat
     8461 tacccgcctg cccggtgagc ataatgagca ttcgagggag aaaaacgcat gattatttcc
     8521 gcagccagcg attatcgcgc cgcagcgcaa cgcattctgc cgccgttcct gttccactat
     8581 atggatggtg gtgcatattc tgaatacacg ctgcgccgca acgtggaaga tttgtcagaa
     8641 gtggcactgc gccagcgtat tctgaaaaac atgtccgact taagcctgga aacgacgctg
     8701 tttaatgaga aattgtcgat gccggtagca ctggcaccgg tgggtttgtg tggcatgtat
     8761 gcgcgtcgtg gcgaagttca ggcagccaaa gcggcggacg cgcatggcat tccgtttact
     8821 ctctcgacgg tttccgtttg cccgattgaa gaagtcgcgc cagccatcaa gcgcccaatg
     8881 tggttccagc tttatgtact gcgcgatcgc ggctttatgc gtaacgcgct ggagcgagca
     8941 aaagcagcgg gttgttcgac gctggttttc accgtggata tgccgacacc gggcgcacgc
     9001 taccgtgatg cgcattcagg tatgagcggc ccaaatgcgg caatgcgccg ctacttgcag
     9061 gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga acggtcgtcc acatgattta
     9121 ggtaatatct cagcttatct cggcaaaccg accggactgg aagattacat cggctggctg
     9181 gggaataact tcgatccgtc catctcatgg aaagaccttg agtggatccg cgatttctgg
     9241 gatggcccga tggtgatcaa agggatcctc gatccggaag atgcgcgcga tgcagtacgt
     9301 tttggtgctg atggaattgt ggtttctaac cacggtggcc gccagctgga cggtgtactc
     9361 tcttccgctc gtgcgttgcc cgctattgca gatgcggtga aaggtgatat cgccattctg
     9421 gcggatagcg gaattcgtaa cgggcttgat gtcgtgcgta tgattgcgct cggtgccgac
     9481 accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa cagcgggcca ggcgggtgta
     9541 gctaacctgc taaatctgat cgaaaaagag atgaaagtgg cgatgacgct gactggcgcg
     9601 aaatcgatca gtgaaattac gcaagattcg ctggtgcagg ggctgggtaa agagttgcct
     9661 acggcactgg ctccgatggc gaaagggaat gcggcatagt cgtttgcgcc ccctcaccct
     9721 aaccctctcc ctcagggaga ggggaccgtt cggcgctgta tgtactccct cactctgaaa
     9781 cgacaccgca ctcttttttt ctccctcgcc cctccgggga gggccggggt gaggggaaaa
     9841 ggccgcacgg ttcaccaatt ttctgctatc ctgcccccgc actaaggggg cagtatgcta
     9901 aacatcgtac tttacgaacc agaaattccg ccaaatactg gcaacatcat ccgtctttgc
     9961 gctaataccg gctttcgtct acatatcatc gaaccgatgg gatttgcctg ggacgataag
    10021 cgcctgcgcc gcgcggggct ggactatcac gagtttaccg ccgttacgcg tcatcatgac
    10081 tatcgcgcgt tcctcgaagc agaaaatccc cagcgcctgt ttgccctcac cacgaaaggt
    10141 acgcctgctc acagcgccgt aagctatcag gatggcgatt atctgatgtt cggcccggaa
    10201 acacgcggcc tgccagcgag cattcttgat gccctgcccg ctgaacaaaa aattcgcatt
    10261 ccgatggtgc cggacagccg cagcatgaat ctgtccaatg cggtgtcggt agtagtgtat
    10321 gaagcctggc ggcagctggg atatccggga gcgttgttga gagattagtt actgtatgcc
    10381 ggatagatga ttacatcgca tccggcacga tcacaggaca ttagatccca tccccatact
    10441 caaatgtatg gttaataccg ttgaaatgct ggtccatatc cattgatggc ttatcgctgt
    10501 ctggtttacc gacaatacga gccggaacgc cagcggcggt tgtatgcggc ggcaccggtt
    10561 gcagcaccac ggaacctgcg ccaatcttcg cgccgcgacc aacttcaata ttgccgagga
    10621 ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt cgggtgacgg tcaccaccag
    10681 atttacccgt accgccaagc gtcacagatt gcagaatcga tacgtcgttt tcaattaccg
    10741 ccgtttcacc cacaacgatg cccgtggcgt ggtcgagcat aatgccacgg ccaatttttg
    10801 ctgccgggtg aatatcgacc tggaacgtca cagaaacctg gttttgcaga aagattgcca
    10861 atgcgcgacg cccctgattc cacaaccagt gaccgatgcg ataggcctgc aaggcatgaa
    10921 aacccttcag gtataacaac ggggttgagt atttatcgac tgccgggtcg cgggtacgca
    10981 ccgcctgaat atcacaggcc gcagaggcga tcatttccgg gtcagcggcg taggcttctt
    11041 caaccacttc acggatagaa atagcaggca taattggcga tgacagcttg ttcgccagca
    11101 tgtagctcag tgcactgcca aggttttcgt gcttgagtag cgtcgcgtgg taaaaactgg
    11161 ccagcattgg ctcacagtcc gccagcgttc tggcttcggc tttaatattg ttccagacaa
    11221 tttccagttc ttcacacgac attgcttact ccacacgatg agataatgac cggcccgttc
    11281 tgcgcgggcc gggtcatagc ggtaacaaag gttccctggg gttagtggct gctgcgctcg
    11341 tccttgcgtg cacgacctaa taatgtcaat gctgcctcgc gcgcgttttt tccgcaatat
    11401 aatacttgat aaatttcctc ggttattggc atttcaacgc cgaagcgatg cgccagttcg
    11461 cggacttctt tcgtattgcg gtagccttcc accacctgac caatcttctc ctgcgcgctt
    11521 tgtacatcca tgccctgacc gagcatcatg ccaaaacggc ggttacgcga ctggttgtcg
    11581 gtacaggtaa gcaccagatc gcccagcccc gccatgccca taaaggtggc aggatcggca
    11641 cccagcgccg caccaagacg cgacatttca gccagcccac gggtgatcag cgccgtacgc
    11701 gcattcgcac caaaaccgat accgtcagac atccccgcgc caatggcaat gacgtttttc
    11761 accgcgccgc caagctgcac gccaatgaaa tccgggttgc tgtaaacgcg gaaacttttg
    11821 ccgcagtgca gcagctgctg gagatcatcg gcaaaggtct gatcggtcga ggccagcgaa
    11881 atagctgtcg gtaaacctgc cgccagttct ttcgcaaacg ttgggccaga gataaccgcc
    11941 agcggaattt gatcgcctaa ggcctcacgc gccacgtcct gtaacagacg tccggtttcc
    12001 gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag gacgcatcag cggtttaatc
    12061 tggcgcagca cttcaccaaa gacatggctg ggtacgacga cgagaatatt acgactggct
    12121 gccagcgcag tggcgagatc gctttcaaga tggagcgtat cgggaaaagg cacatcgggg
    12181 agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga tatgttcagg gtcatggccc
    12241 cagaggacaa cctcgtggcc atttcttgcc agggtaatgg caagagcggt gccgtacgag
    12301 ccggcaccga tcacagtcat tgaagcatta cgttggttca tcaggcatcc tgatgttctt
    12361 cagtaccttc gccagcctgc tgctgcaaat agttcatgaa cagcgcatcg aagttaaccg
    12421 gcgcaaggtt cagttgcggg aatgtaccgc gggataccat gctggtgatg cactcacgag
    12481 catacgggaa cagaatgttc gggcagtatg cgcccaggca atgtgccatc tgggtgcctt
    12541 cgatacccgc gatggagaaa ataccgccct gctgaacttc acacaggaac gcggtttctt
    12601 cgcccaagga ggccgttacg gtaacacgca gtaccacttc gtatacgtca tctgccagtt
    12661 gggaagatgc cgtatccaga tcaagtttaa cttctggttg ccaatctttc tggaaaacgt
    12721 gcggcgcgtt cggcgcttcg aaagagatat ccttggtata aatacgttgg atctggaaag
    12781 tcatttcagt gttgttttgt tctgacatgt gtagaaaacc cttaagtgtt gtccttaaat
    12841 actgcgcaat gtaatgccaa cgttcacacg ttatttcagc aggggatcca gtccaccacg
    12901 tgcatccaat gcatacaagt catcacagcc gccaatgtgc tgtgcgtcaa taaaaatctg
    12961 gggaaccgtg gtgcgaccgc tgcgtttgat catctcttca cgcttggcgg cattgccatc
    13021 gatcggcaac tcctggaaac tcacgccctt gctgctcagc agtgcttttg cacgatggca
    13081 atacgggcag gtttctttgg tatagatttc aacattggcc ataatttagc tcctgttttt
    13141 ttaccctgaa atttcatgtt gcagggcggc tgcaaaatgg agtgtcccca gaagcttata
    13201 acaataagtc gctgggggcc taattgcagc taacggcctg catcatgaaa gacgacaggt
    13261 aaattatttg ccgcgcacca gaggcaagtt ttcgccagcc cagccagcaa cgccctcttt
    13321 cagcacgaat acttgcgcaa aaccagcttt cgtcagcgcg tttgcaggct cctggcactg
    13381 catgccagaa ccgtctacca cgataaccgg tttgtctttg tgcttctcaa gctcaccaac
    13441 attgttggct ttgatttcgc tcggcaacag attgatagaa cctgcgatat ggcctttacg
    13501 gaagtcatca cgctgacgta aatccacaac cacagcgtct tctttgttga tcagacgcgt
    13561 agcttcacca cgagtaatca ccttcacttt cgaggtcagg ctcttaaacg tagtcacaag
    13621 aaccgccacc agtaacgcga tccaggcgat actcagtatg ggatgacggc caacaaattg
    13681 cataatttct tgcatggggg gtaacaactc ccgacgtagt gattaaaaaa accaggaaag
    13741 gagtatacct gcgcggtgcg gcaaatacag ccagcgcgtt aactggaatg caattttgcg
    13801 gggcacgacg aaaaaaaagc gcaaatctgg catcggcttg ccgcgagcgg tcgtatattt
    13861 tgatcttcag aggctatttt atcgattcag ctgtagtaaa attacgcaaa ttttgactct
    13921 tgagtatgag gttgtcgcaa tgtcggtttc taaaaaacct atggtactgg tgattctgga
    13981 tggctatggc tatcgtgaag aacaacagga taacgccatt tttagtgcta aaaccccggt
    14041 aatggatgca ctgtgggcca atcgtccgca taccctgatc gacgcttccg gtctggaagt
    14101 cggtctgcct gaccgtcaga tgggtaactc cgaagtaggc cacgttaacc tgggtgccgg
    14161 ccgcatcgtg tatcaggacc tgactcgtct ggacgttgaa atcaaagatc gcgctttctt
    14221 tgctaatcca gtgctggctg gcgcggtaca taaagcgaaa aacgcaggta aagcagtaca
    14281 cattatgggt ctgctctccg caggcggcgt acacagccac gaagatcaca tcatggcgat
    14341 ggtagaactg gcagctgaac gcggcgcaga aaaaatctac ctgcacgcat tccttgacgg
    14401 tcgcgacacc ccgccgcgca gtgctgaatc ctcgctgaaa aaatttgaag agaaatttgc
    14461 tgcgctgggt aaaggccgcg tagcgtccat cattggtcgt tactacgcga tggaccgcga
    14521 taaccgttgg gatcgcgttg aaaaagccta tgacctgctg actctggcgc agggtgagtt
    14581 ccaggccgat actgccgttg ctggtttgca ggcagcttat gctcgcgacg aaaacgacga
    14641 gttcgtgaaa gcgaccgtta ttcgtgcaga aggtcagcca gatgcggcaa tggaagacgg
    14701 cgatgcgctg attttcatga acttccgtgc tgaccgcgcg cgtgaaatca ctcgtgcatt
    14761 cgtgaacgct gatttcgatg gcttcgcgcg taagaaagtg gttaacgtcg atttcgtgat
    14821 gctgaccgaa tacgccgctg acatcaaaac tgcggttgct tacccacccg cttccctggt
    14881 taacaccttc ggcgagtgga tggcgaaaaa cgacaaaact cagttgcgta tttccgaaac
    14941 cgaaaaatat gcccacgtta ctttcttctt caacggtggc gtagaagagt cgttcaaagg
    15001 cgaagatcgc attctgatca actcaccgaa agtggctacc tacgatctgc aaccggaaat
    15061 gagctccgca gagctgaccg aaaaactggt tgcggccatc aagagcggca aatacgacac
    15121 cattatctgt aactatccga acggcgacat ggtaggtcac accggggtga tggaagcggc
    15181 ggttaaagca gttgaagcac tggatcactg cgtggaagaa gtcgcgaaag cggttgagtc
    15241 cgtgggtgga caactgctga tcaccgctga ccacggtaac gctgaacaga tgcgcgatcc
    15301 ggcaacgggt caggcacaca cggcacacac caacctgcca gttccgctga tttacgttgg
    15361 tgataagaac gtgaaagcgg ttgcaggcgg caaactttct gacatcgcgc cgaccatgtt
    15421 gtcgctgatg ggtatggaaa tcccgcaaga gatgactggt aagccgctgt tcatcgtgga
    15481 ataatccctc cccatgaggg gaaaggcgat taataccatg acacgggccg tgaaaccgcg
    15541 caggtttgca atcaggccca tcatctacgc cagcgttctt agcgctggcg tattgttgtg
    15601 cgccttttcc gcccacgcgg atgagcgtga ccaactcaaa tctattcagg ccgatatcgc
    15661 cgcaaaagag cgcgcggtac gccaaaagca acaacaacgc gcaagcctgc tcgcacaatt
    15721 gaaaaagcag gaagaagcga tctctgaagc cacccgtaag ctgcgcgaaa cgcaaaacac
    15781 gctcaatcaa ctcaataaac agattgatga gatgaacgcg tcgattgcca aactggagca
    15841 gcaaaaagcc gcccaggagc gcagcctcgc cgcacaactg gatgccgcat tccgtcaggg
    15901 cgagcatacc ggtattcagc taattctcag cggtgaagaa agccagcgtg gacagcgttt
    15961 acaggcttat ttcggctatc tcaaccaggc gcgacaagaa accattgccc agttgaagca
    16021 aacgcgtgaa gaagtcacca tgcagcgtgc tgaactggaa gagaaacaaa gcgagcaaca
    16081 aacgctgtta tatgaacagc gcgcccaaca ggcgaaactg actcaggctc tgaacgagcg
    16141 taaaaagacg ctggcggggc tggaatcttc catccagcaa ggtcagcagc agctaagtga
    16201 actgcgtgcc aacgaatccc gtctgcgtaa cagcattgcc cgtgcggaag ccgcggcgaa
    16261 agcgcgtgct gaacgagaag cgcgtgaggc ccaggcggtt cgcgagcgcc agaaagaagc
    16321 gacgcgcaaa ggcaccacct acaaaccgac cgaaagcgaa aaatcgctga tgtcccgaac
    16381 tggtggcctg ggtgcgccgc gtggtcaggc attctggccg gttcgcgggc cgacgctgca
    16441 tcgctatggc gagcagctac agggtgaatt acgctggaaa gggatggtta tcggtgcctc
    16501 tgaaggtact gaagttaaag caattgccga cggtcgagtg attctggctg actggctgca
    16561 aggttacggt ctggtggtgg tggttgagca cggtaaaggc gacatgagtc tttacggcta
    16621 taatcagagc gcactggtga gcgttggttc gcaggttcgc gcgggccagc caattgcact
    16681 ggtgggcagc agtggcggtc agggtcggcc ttcactctat ttcgaaattc gccgccaggg
    16741 tcaggcggtc aatccacagc cgtggttggg aagataagtt ttgtttccat ttcgtcgtaa
    16801 cgttcttgca tttgccgctc tgttggcgct ctcctccccc gtacttgctg gcaaacttgc
    16861 catcgtcatt gatgattttg ggtatcgccc gcacaacgaa aaccaggtgc tggcgatgcc
    16921 ttccgctatc tccgtcgctg tattacccga ttcaccgcac gccagagaga tggcgaccaa
    16981 agcgcataac agcggtcacg aagtgttgat tcatctcccg atggcaccgt tgagtaaaca
    17041 gccgctggag aaaaatacgc tacgcccgga gatgagcagc gacgaaattg agcgcattat
    17101 tcgtagtgcg gtcaataacg tgccctatgc cgtggggatc aacaaccaca tgggcagcaa
    17161 gatgacctct aacctgtttg gtatgcagaa agtgatgcag gcgctggagc gttacaatct
    17221 ttacttcctc gacagcgtaa ccatcggtaa tacccaggcg atgcgcgccg cgcaaggtac
    17281 tggcgtgaag gtgatcaaac ggaaggtgtt cctcgacgat tcgcagaacg aagcggacat
    17341 ccgtgtgcaa tttaatcgcg caattgatct ggcgcgtcgc aacggttcaa ccattgccat
    17401 tggacatcct cacccttcaa cggtacgcgt gctacaacag atggtttata acctgccgcc
    17461 agacattacg ctggtgaaag ccagcagctt gctgaatgaa ccgcaggttg atacttctac
    17521 accgccgaaa aacgctgtgc ctgatgcacc gcgtaatccg ttccgtggcg tgaagctgtg
    17581 caaaccgaag aaaccgatag aacctgttta tgctaatcgc ttctttgaag tattaagcga
    17641 aagcatcagc cagagcacgc tgatcgttta cttccagcat cagtggcaag gctggggcaa
    17701 acagcccgaa gcggcgaagt ttaacgctag cgcaaattaa aggcgctacg cgtaatgcga
    17761 tggtgcgacc gcgttttgtc gcgccattgc cataagcgga aagaccacag taacgcctgg
    17821 taaccgacct tcacgctgcg cacattggta atcaggcgct tatacatacc ggaagtaaat
    17881 atctcggcaa tcatccgttg gcgggtaaga atatccggct ctttgcgcac cgcatggcaa
    17941 acacgcaatg cttcgtaagt tatttgctga tgaaattccg gataaatcgt aattttgtcg
    18001 gcataatttc gatttaattt ctccagcagg cgggtaatct taatatagtg acgttgataa
    18061 ttaaggtttt tattcccttg tctatgcaac cgactcactg acgtattatg cagataatat
    18121 ttatataatg attgctcggt atatcgcgca cgcagcgcgt taaacatgaa ttctgttgtc
    18181 cagacaatat cctgatgatg taatccggca ataaatttaa tgttattttt aacaataaca
    18241 tcacggcgat aaacccccat ccagacaacg tgagtccaac ggcgcgaaaa aagccccatc
    18301 cgcagccagt ccgggccggt taatacaccg gttgagcgaa ggcgatcgat ggggatggat
    18361 tgccaagttt ctcccgtttc acgaaaagac cagtcagcgt tgcactgcgc cacgtcgagg
    18421 tcgtcctcta acgccatggt catcagcgtt tcgtacatgg tgggatagac ctcatcgtca
    18481 gcatcgacaa aagcgacata tttgcccgtc gccacctcga tcccacgatt acgcgctacc
    18541 gatgcgccag cattcgcctg atgcaacaaa cgaacgtgcg gatagttttc tgcgtaatgc
    18601 tttgctattt caacagaatt atccgttgaa ccatcgttaa taataatgat ttccagagca
    18661 gtccaggttt gcgtaattaa agattccata caagtgcgga aatcatcgcc cgcattatat
    18721 aacggaataa taacactaag tttattggtg ctgttcatca taaattaccg acgacctctg
    18781 aagttatcct gacgttttac aggctttgta ttaagaaact attaagcctg tgagatatta
    18841 tttttacagg tgagagagtc acttcagggt agagagataa tgagaacagc tatataaaag
    18901 atcagcgcaa ttacgaactc tttattacac tgacagga
//
LOCUS       DADLSB010000074        18564 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.074, whole genome shotgun sequence.
ACCESSION   DADLSB010000074 DADLSB010000000
VERSION     DADLSB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.074"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(193..287)
                     /locus_tag="JN331_002407"
     tRNA            complement(193..287)
                     /locus_tag="JN331_002407"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Sec,seq:tca)
     gene            580..1963
                     /locus_tag="JN331_002408"
                     /pseudo
     CDS             580..1963
                     /locus_tag="JN331_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            1973..4291
                     /gene="yicI"
                     /locus_tag="JN331_002409"
     CDS             1973..4291
                     /gene="yicI"
                     /locus_tag="JN331_002409"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY7260534.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFCFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(4344..6053)
                     /locus_tag="JN331_002410"
     CDS             complement(4344..6053)
                     /locus_tag="JN331_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7260535.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTSLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(6174..7565)
                     /gene="xanP"
                     /locus_tag="JN331_002411"
     CDS             complement(6174..7565)
                     /gene="xanP"
                     /locus_tag="JN331_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY7260536.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7845..9050
                     /gene="gltS"
                     /locus_tag="JN331_002412"
     CDS             7845..9050
                     /gene="gltS"
                     /locus_tag="JN331_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY7260537.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9084..11165)
                     /gene="recG"
                     /locus_tag="JN331_002413"
     CDS             complement(9084..11165)
                     /gene="recG"
                     /locus_tag="JN331_002413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY7260538.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11171..11860)
                     /gene="trmH"
                     /locus_tag="JN331_002414"
     CDS             complement(11171..11860)
                     /gene="trmH"
                     /locus_tag="JN331_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY7260539.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAAQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11867..13975)
                     /gene="spoT"
                     /locus_tag="JN331_002415"
     CDS             complement(11867..13975)
                     /gene="spoT"
                     /locus_tag="JN331_002415"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY7260540.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(13994..14269)
                     /gene="rpoZ"
                     /locus_tag="JN331_002416"
     CDS             complement(13994..14269)
                     /gene="rpoZ"
                     /locus_tag="JN331_002416"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY7260541.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQESAELQAVTAIAEGRR"
     gene            complement(14324..14947)
                     /gene="gmk"
                     /locus_tag="JN331_002417"
     CDS             complement(14324..14947)
                     /gene="gmk"
                     /locus_tag="JN331_002417"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY7260542.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15205..16887
                     /gene="ligB"
                     /locus_tag="JN331_002418"
     CDS             15205..16887
                     /gene="ligB"
                     /locus_tag="JN331_002418"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY7260543.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(16884..17501)
                     /locus_tag="JN331_002419"
     CDS             complement(16884..17501)
                     /locus_tag="JN331_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY7260544.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(17792..>18564)
                     /gene="dinD"
                     /locus_tag="JN331_002420"
     CDS             complement(17792..>18564)
                     /gene="dinD"
                     /locus_tag="JN331_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY7260545.1"
                     /translation="AEQWSARQLGKLLGYSEYRHFIPVLTRAKEACENSGHTIDDHFE
                     EILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVIAAGQTYFTIQTRRQELAD
                     DEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATATDFAIFQNHGYQGLYGGLD
                     QKAIHQLKELKKSQKILDHMGSTELAANLFRATQTEEKLKRDSVNSKQQANTTHFDVG
                     SKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
BASE COUNT         4242 a         4705 c         5011 g         4606 t
ORIGIN      
        1 ccgttgttat gtatcacatt tttcctatta tgggggtgct tgtatttcaa taggaaataa
       61 caccccctaa agtaccccca aaagaaactt gatacggggt gaagtaggtt gacctcagga
      121 tacaaataaa tactgatata caagggatta gaagagattt attgacattg ggagagttca
      181 gaagaggtaa actggcggaa gatcacagga gtcgaacctg cccgggaccg ctggcggccc
      241 caactggatt tgaagtccag ccgcctcacc ggagacgacg atcttccgcg cctcgattgc
      301 tacatggagg cggggcgcat tatagctact tccttgagtt tctacatccc ccagatcagt
      361 tttttccgcc taataaacgc agcgtattat cacgaaagca ttataattca tataaaaccc
      421 atattttaca atgatatatg agcggaatta acgctaccac gatcacataa aacaagaatc
      481 ataaattaat aaccagatat cggaatattc gctctcacag ggatggttta caaaatgcgt
      541 tatcggtctc agcaccccta tagcatcaag gaaaagcaga tgaagagtga agtgttgtcc
      601 gttaaagaga aaattggtta tggcatggga gacgccgcca gccacattat tttcgataac
      661 gtaatgttat atatgatgtt cttttatacc gatatttttg gcattcctgc cggatttgtc
      721 ggaaccatgt ttttggtcgc tcgtgcactg gatgcgattt ccgatccttg catggggttg
      781 ttggccgatc gaacgcgctc tcgctggggt aaatttcgtc cgtgggtact gtttggcgca
      841 ctgccattcg ggatcgtctg tgtactggcc tatagcacgc cagatctcag tatgaacggc
      901 aaaatgatct atgcagcaat tacttacacc ctaacttacc ttactttata ccgtcgtcaa
      961 tatcccttac tgcgcattgg gtggtgtaat caccaatgac ccgactcagc gtatctcgct
     1021 gcaatcctgg cgttttgtgc tggcgaccgc gggaggcatg ctttctactg ttctgatgat
     1081 gccactggtt aatttaattg gcggtgataa taaaccactc ggtttccagg gcggtatcgt
     1141 ggtcctttcc gtggtggcat tcatgatgct ggcattttgt ttcttcacca ctaaagaacg
     1201 cgttgaagca ccacctacaa caacgtctat gcgggaagat ttacgtgata tctggcaaaa
     1261 cgaccagtgg cggattgtcg gtttactaac cattttcaat atcctagcgg tgtgcgtacg
     1321 cggtggggcg atgatgtatt acgtcacatg gattttgggc acgccggaag tgtttgtcgc
     1381 ttttctcacc acttattgcg tgggtaacct gattggttcc gcactggcaa aacctctgac
     1441 cgactggaaa tgtaaagtca ctatcttctg gtggacgaac gccctgctgg cagtgattag
     1501 cctcgcgatg ttctttgttc ccatgcaggc cagcatcact atgtttgtct tcatcttcgt
     1561 gattggtgtg ttgcatcaac tggtgacacc tatccagtgg gtaatgatgt ccgataccgt
     1621 cgactacggc gagtggtgca atggtaaacg cctgaccggg atcagttttg ctggcacgct
     1681 gtttgtgctc aaactggggt tggccttcgg cggcgctctt atcggctgga tgctggctta
     1741 tggcggatat gatgcggcag aaaaagcgca gaacagcgcc acgattagca tcattattgc
     1801 gctattcacg attgttccgg cgatctgtta tttgctgagc gcgattatcg ctaaacgcta
     1861 ctactcactc acgacgcaca atctgaaaac cgttatggaa cagctggctc agggtaaacg
     1921 ccgttgccag caacaattca cctctcaaga agtgcagaac taaggaacgg caatgaaaat
     1981 tagcgatgga aactggttga ttcaacctgg cctcaatttg attcacccgc ttcaggtatt
     2041 cgaggttgaa cagcaggata atgaaatggt ggtctatgct gccccccgtg atgtgcgtga
     2101 acgtacctgg cagcttgata cgcctttatt tacgctgcgc tttttctccc cacaggaagg
     2161 tattgtcggt gtgcggattg agcattttca gggggcgctg aataacggtc ctcattatcc
     2221 gctcaatatt ttgcaggatg tgaaggtcac aatcgaaaac acagaacgtt atgctgagtt
     2281 taaaagtggc aacttaagcg cgcgtgtcag caaaggtgag ttctggtcac tggattttct
     2341 gcgcaacggc gaacgtatta ccggtagtca ggtgaaaaat aatggctacg tgcaggacac
     2401 gaataatcaa cgcaattata tgtttgagcg gcttgatctt ggcgttggcg aaacagttta
     2461 cggtctggga gagcgcttta ctgccctggt gcgcaatggc cagacggtag agacctggaa
     2521 ccgggacggc ggcacaagta ctgaacaggc gtataaaaat atcccgttct acatgactaa
     2581 ccgtggttat ggggtactgg tcaatcatcc ccagtgtgtc tcttttgaag tgggatcgga
     2641 gaaagtctcc aaagtgcagt tcagcgttga gagtgaatat ctcgaatact ttgttatcga
     2701 cggcccgacg ccgaaagcgg tacttgatcg ttatacccgc tttactggtc gtccggcgct
     2761 gccgcccccg tggtccttcg gcctgtggct aaccacttca tttaccacca actacgacga
     2821 agcgacggta aacagcttta tcgatggtat ggcggaacgc aatctgccgc tgcatgtttt
     2881 ccactttgac tgtttctgga tgaaagcctt ccagtggtgc gattttgagt gggacccgct
     2941 gactttccct gacccggaag ggatgatccg ccgcctgaaa gcgaaagggc tgaaaatctg
     3001 cgtctggatt aacccctata tcggtcaaaa atcccccgtc tttaaagagt tacaagagaa
     3061 aggctattta ctcaaacgcc cggacggttc gctatggcag tgggataaat ggcagccagg
     3121 tctggcgatt tatgacttta ccaatccgga tgcctgcaaa tggtacgccg acaaactgaa
     3181 aggtctggtc gcgatgggcg ttgattgctt taagaccgac tttggcgaac gtatcccaac
     3241 tgatgttcag tggtttgacg gttccgatcc gcagaaaatg cataaccatt atgcgtacat
     3301 ctacaacgaa ctggtgtgga acgtgctcaa ggacaccgtt ggtgaggaag aagctgtctt
     3361 gtttgcccgc tcggcctccg tcggtgcgca gaaattcccg gtacactggg gtggcgattg
     3421 ttacgctaac tacgaatcaa tggcggaaag cctgcgcggt ggtttgtcta ttggcctttc
     3481 aggtttttgc ttctggagcc acgatatcgg cggctttgaa aataccgctc cggcgcacgt
     3541 ttacaaacgc tggtgcgcgt ttggtttgct ctccagccat agccgtttac acggtagcaa
     3601 atcttatcgt gtgccgtggg cctacgatga tgagtcctgt gatgtggtgc gcttcttcac
     3661 gcaactgaaa tgccgcatga tgccgtatct gtatcgtgaa gctgcgcgtg cgaacgcgcg
     3721 gggtacgccg atgatgcggg ccatgatgat ggagttcccg gacgatccgg cttgtgatta
     3781 ccttgaccgt caatacatgt taggcgacaa cgtgatggtt gcgccggtgt tcactgaagc
     3841 gggcgatgtg cagttctacc tgccggaagg tcgctggaca cacctgtggc acaacgatga
     3901 actcgacggt agtcgctggc ataaacagca gcacggcttc ctgagtctgc ccgtttatgt
     3961 gcgtgataac actctactgg cgctgggcaa caacgatcaa cgtcccgatt acgtgtggca
     4021 cgaaggcacg gcattccacc tctttaatct gcaagacggg catgaagccg tctgtgaagt
     4081 gcccgctgct gacggatcgg tgatctttac tttaaaagca gcacgtactg gcaacacgat
     4141 tactgtgact ggtgcgggcg aggcgaagaa ctggacgctg tgcttgcgca atgttgtgaa
     4201 agtaaatggt ctgcaaggcg gttcgcaggc tgaaagtgag caggggctgg tggtgacgcc
     4261 tcaagggaat gcgctgacaa ttacgttgta atctattgac ggaatgccgg gtacgtctga
     4321 gtcgtacccg gcgagattga tgtttaccgc accggctccg tcgatgtaac ttcaccgctg
     4381 gaaacgacgc ccgcattcat ggtttgagtc acttgctgct ttgcggcgtt cacggcatgc
     4441 aattgcccgc taaccgtagg tttcagcggg gcattggcct gaatatctcc actggcggta
     4501 agctgaatat tgccgtcgcc agtcaacggt aattcaggcc atccccattg ttgcaaaata
     4561 ttcaccggca caccgcgtcc attcaggctg atatgggtct gacgttgtgg cgtttgtgaa
     4621 acacttgcgg tggcttccag aatgcctttt tcagtaaatg cactcagttc gctgatattc
     4681 accgtgctgc tgttggcggt cagcgccagc gacgggcgac gaacatcaac acgattgaat
     4741 gtcgcggcgg cggcattcag attcgccgag ccactccaga cgccccattt atgatcggta
     4801 accagcgtca ggttggcacc gtaaccatcg agcgaggtga gctgccacgg gaagtcaggg
     4861 tcgatatcaa tgatcagatt gcggctggcg ctaaatctct tcagttgcag gctgtttaac
     4921 caaccgggtg tcgtttccat ccacaactgt tgccagtttt tcggcaaggt atattccagc
     4981 ccggcaattg ccgcatcatc aaggatcaac gttttaccgt cacgcagcca gttccctgac
     5041 gttctgacca tacccccttc ccagcggctg gtgaactggc gcagcgctac gccctgcggg
     5101 gaaaattccg cgtttataat cgggtcaaat aaatgcagcg aaccataaat gaactcgctg
     5161 gcgttcatcg acagtttgcc ttcctgtgtc tgccagtcat ctttactgaa ggtcatgttg
     5221 cgcaagctga gatcgaggtc ggtcaccgcc cagtccggac cttgcagacg agcatcgatg
     5281 acttccaagc gaccaatctg caacgacggg acagagcgta atggcgcaaa gaagtcggtt
     5341 agcgattttt cgctttgtag acggatatcc gccatgcgca gattttccac ttgccagctg
     5401 ccgtcggcgt tacgctgcgc gtttccggtt aatgtcccgc gggcgatgtc ggcacccagg
     5461 ttagtcagcg taacgcgatc gttatcaata ctgccttcaa tcagtacatt ggtggcagga
     5521 acatcattga gcgaaagcga tccggcacta aactgaatct gcgccttcgt acccagcact
     5581 ttaccggctt ctggtgacca cggaaccacg ccgccattta cccgctgcgc gctcagtttc
     5641 cattcgctat tcgggctatt aaacgccata tcacgcagtt gcagacgatc ggctttgaac
     5701 ggtagcggcg cggtctggtc ggtgagattc agcgtcccgt tttccagcag gatggtatcg
     5761 acatggcgtg gttcggtcag ttggcgactg cttagcgcaa tgtcgacact ttttgccacc
     5821 aggggcgcgg gctgaccatc acgaccaaac gtgacattct ccagcacgat atgagatggc
     5881 gcggaaaaac ggtgatccat cgccccgaag gccagatgat agtcgctatt ctcggaaacc
     5941 catgcgctga tatgttctgc tccccagcgg gtttgcagaa gaaaatagag gccagcgatc
     6001 accactaaca gagcgatgag aatgtagaga agcagcttcc caataaattt catggtcttc
     6061 catcccgtga aatgcacata agggagttat gcacgattta cgcgcaatcc tcaaggcggg
     6121 aatggtgaaa gaggtgagac tgcggcggta attatcttac cgccgccagt gaattactgt
     6181 ttttctggtg ggaaaatcag attcagcacg atggcagtaa taccgcccgc ggcgatcccg
     6241 gaggagagca ggtttttcag ccattcaggg gcaaactgca aaatcagcgg ctgctgagac
     6301 acgcccagac caaccgccag cgacagcgcg ataatcagaa tcgcccgacg gttcagcggc
     6361 tcacgagaaa cgatacgcac accggaggcg gcgatggtgc caaacattac aagcgttgcg
     6421 ccgcccagaa ccggttctgg aatgtgttgt acaaaaccgc tcactgccgg gaacagaccc
     6481 agcacgatca acatcagcgc gacgacaaaa ccgacatagc ggctggcaac accagtcaac
     6541 tggatcactc cgttgttttg cccgaagcag gagttcggga aggtgttaaa caccgccgaa
     6601 acaaacgagt tcaggccgtt tgccagcacg ccgcctttca ggcgtttcat gtacagcgga
     6661 ccggacactg gctgttcgga aacgtcagag gtcgccgtga tatcgccaat ggtttccagc
     6721 gaagtgatca taaagaccag catcagcggc agcagcagac tccattcaat gccaagaccg
     6781 taatagagcg gcgttggcac cataatcagt tcttgcgtca tcggttcgtt gctttctggc
     6841 aacatgccca taaaccacgc cagcgcatat ccggccgcca tcgcaattac cagtgaggcc
     6901 acgcgtaagt aagggttacg ttgacggtta agcaggataa ttaaggctaa aaccacgcct
     6961 gccagcagca gatttttcgg tgcgccgaag gtgttatcgc tcatggctgc gtaaccgccg
     7021 ccaatggagg tcagccccac ctgaattagc gacaggccga taatcatcac cacaacgcca
     7081 gaaaccagcg gcgtaataat gcggcgcgcc agatgcagaa cgcgggagat caccatctcg
     7141 gtgcaacttg ccagcatcaa cgtgccgaac aaagccgcca tcatggtagg aacatcagca
     7201 ccaccggttt tcagcgcggt accgcccata atcagcgggg caacaaagtt gaagctggtg
     7261 ccctgaatag acaacagccc ggagccaacc ggaccccagg ccttaatttg aataatcgat
     7321 gccacaccgg aggcaaacag cgacatacta ataatgtgtt gcgtgtcttg tgccggtaaa
     7381 cccagcgcct ggcagattaa tagcgctggc gtgatcaccg caacgaacat cgccagcaga
     7441 tgctgacagg cggcaaacag ggtttgagga agcggcggac gatcttcaag acggtaaatc
     7501 agttcgctgt tttgagtctg cgcaaccggt tgcgcatttt ctgactcgag ggtggaaaca
     7561 gacatcgaaa acaatcccgt ggtagcaaag cgggcatttt agctgaccga aagagaaaag
     7621 caaacgtttg ccacatcctc atctaaatat ttaaatgaga taaatgcact tttttatata
     7681 acttttggta tttttctgct taaaatccat gccatttgtt cattaacagc tgagaatttg
     7741 acccaggaaa gcataactcg atacccgtca tagttcatga agttgccact gcgcgatgaa
     7801 gtatgacgag tatgaaagag tgatgcggat acaaaggagt aactatgttt catctcgata
     7861 ctttagcaac gcttgttgcc gcaacgctga cgttgctgct cgggcgtaag ttggtccatt
     7921 ccgtctcctt tttgaagaaa tacaccatac cggaacctgt tgcgggtggt ttgttggtgg
     7981 cgctggcgct actagtactg aaaaaaagca tgggctggga agtcaacttt gatatgtccc
     8041 tgcgcgatcc gttaatgctg gctttcttcg ccaccattgg cctgaacgcc aacattgcca
     8101 gtttgcgtgc cggtgggcgt gtggttggca tcttcttgat tgtggttgtt ggtctgttgg
     8161 tgatgcaaaa tgccattggc attggtatgg ctagcctgtt agggcttgat ccgctgatgg
     8221 ggctgttggc cggttctatt acgctttccg gcggtcacgg tacgggcgct gcgtggagta
     8281 aattgttcat tgaacgttat ggcttcacca atgcgacgga agtggcgatg gcctgtgcaa
     8341 cgttcggtct ggtgctgggc ggcttgattg gcggtccggt ggcgcgctat ctggtgaaac
     8401 actccaccac gccgaacggt attccggatg accaggaagt cccgacggcg tttgaaaagc
     8461 cggatgtggg acgcatgatc acctcgttgg tgctgattga aactatcgcg ctgattgcta
     8521 tctgcctgac ggtggggaaa attgttgcgc aacttttggc tggcactgct tttgaactgc
     8581 cgaccttcgt ctgtgtactg tttgttggcg tgattctgag caacggtctg tcaataatgg
     8641 gcttttaccg cgtctttgag cgtgcggtat ccgtgctggg taacgtaagc ttgtcgttgt
     8701 tcctggcgat ggcgttgatg gggctgaaac tgtgggagct ggcttcgctg gcgctgccga
     8761 tgctggcgat tctggtggta cagaccatct tcatggcgtt gtatgccatc ttcgttacct
     8821 ggcgcatgat gggcaaaaac tacgatgcgg cagtgctggc tgcgggtcac tgtggttttg
     8881 gcctcggtgc aacgccaacg gcaatcgcca acatgcaggc gatcactgaa cgctttggcc
     8941 cgtcgcacat ggcgtttttg gtggtgccga tggtcggtgc gttctttatc gatatcgtca
     9001 atgcgctggt gattaagctg tatttaatgt tgccgatttt tgccggttaa ccgatgaagc
     9061 ggcggtcttc tcaccgccgc cttttacgca ttcgagtaac gttctgtctc cggcatccag
     9121 cgttctatca aggcttttgc ctgttgtggg taacgttcgt gaatatgtcg cgccaggcgc
     9181 tgaacttccg ggatcatcgc ctgatcgcgc agtaaatccg ccactttaaa ttcagcatta
     9241 cccgtctgac gcgtgcctaa caattcgcca gggccgcgaa tctccagatc tttttgcgca
     9301 atcacaaaac cgtcgttact gtcgcgcagc acttgcaggc gaatttgcgc cgttttagaa
     9361 agcggcgttt tgtagagcag cacgcagtga gaagccaccg cgccacgacc tacgcgcccg
     9421 cgcagctggt gtaactgcgc cagacccaga cgctcagggt tttcgataat catcaggctg
     9481 gcgttaggca catcaacacc gacttcaata acggtagtgg caaccagcag atgtagctca
     9541 ccttgtttaa acgacgccat caccgcctgt ttctcggctg gtttcatccg cccgtgtacc
     9601 aggccaacat tcaattctgg tagcgccagt ttcaactctt cccaggtagc ttccgccgcc
     9661 tgcgcttcca gtaactctga ctcttcaatc aacgtacaaa cccagtacgc ctgacgacct
     9721 tcagttatgc aggcgtggcg cacgcggtca atgatgtcgg tacggcgggt atcaggaata
     9781 gcgaccgtag tcactggcgt gcggcctggc ggcagctcat ctatcaccga ggtatcgaga
     9841 tcggcatacg ccgtcattgc cagcgtgcgg gggatcggcg tggcggtcat gatcaactga
     9901 tgcggatgga agccctgctg ctggcctttc tcccacaatg ccagacgctg atgcacgcca
     9961 aaacgatgct gttcgtcgat aatcaccagc gccaggccgt taaactgcac ctgttcctgg
    10021 aagatggcgt gtgtaccgac aatcatctgc acctgaccgc tggcgatggc ttcctgctga
    10081 gacagtcgcg ctttgccttt ctgcttaccg gcgagccagc ccacttcgat accgagcggt
    10141 gcaaaccagt tgcggaagtt attggcgtgc tgctcggcga gtaattcggt tggtgccatc
    10201 agtgccacct gtttgccgtg ggcaatcgca cgcagcgcgg cgagggcggc gaccagcgtt
    10261 ttaccggaac ctacatcgcc ctgtaccagg cgcatcatcg gcacatccag cgccatatca
    10321 tgctcgatct ccgccactac gcgtgcctgt gcgcccgttg gcttgaacgg taaggcggcg
    10381 aggagtttat ttttcagcgt gtcattggcg ctcagcggct gggcatgaaa acgctgtgct
    10441 ccggcacgta aggctaacat gctgaggttg tgcgccagca gttcttccag aatcagacga
    10501 cgttgcgccg gatgctgccc ggtttccaga tcgctaagct gtagcgtcgg tggcgggcgg
    10561 tgcaaagtgc gcaacgcttc cggtagcgtc atcattcctt gtgacagttc cggcggcagg
    10621 agttcttcaa tggcgcaggt gtcgagcaga tccagcgcct ggtcggttaa tttacgcagc
    10681 gtggcctgct ttacgccttc cgttgttgga taaaccggcg tgagcgtttc ctgtaattct
    10741 ggcgtgctga gatcgccctg cacgcggtat tccgggtgga tcatctccgc accatattta
    10801 ccgcgctttg cttcgccata agccagtaca cggcggcccg tcgccaggct atttttcatt
    10861 gccgcgctga aattgaaaaa gcgcatggtg aggatgccgg aaccgtcgct gatctggcag
    10921 gtcatcatcc gccgaccgcc gaaggagata ttgcagttca gcacttcgcc ttccaccgtg
    10981 gcataaacgc ccggcagtag ttctccgatg gggtagagat gggtgcgatc ttcgtagcgc
    11041 aggggaaggt gtaagagtag atcctgcacg gtatgcaggt tgattttcgc cagtttgtta
    11101 ctaagtgctg cgccaacgcc cgttagggaa ctgagtggga cagcatctaa caggcgacct
    11161 ttcatggcac ttaccctgca gcctgcatag tagcccacca gtcggcatca gcttcgatct
    11221 cgccttgctg attgacgtgg ggataaggca ggcctttgcg ttttgcgact ttcgccagca
    11281 ccggatagcc gccttcaaac aacaggcgtt gttgcgctgc ttccggcaac atgctgtttt
    11341 cacgcaggta catgcctgca ttttgccgct gacgctgggc ttcgtaaaga atgagggctg
    11401 aggcaacgga aacattcagc gactgcacca tgccgatcat cggaatgatg atgtcctgat
    11461 ccgccagggc caatgcttcc tgcgtgatgc ccgttttctc ctgtcccatc aaaatgcagg
    11521 tcgggcgagt gtaatcaatt tcgcggaaat cgacagcatt atcagaaaga tgggttgcca
    11581 gaatctgcat gccctggcct ttgagatgag cgacggcatc gccaatggtg cggtgtgttt
    11641 tcacctgtac ccagctgtta ctacccgccg ctgccgaagc catggtgcgc atgcggctac
    11701 caggccagac ggcgtgaact tcatgtacgc caacggcatc tgcggtacga ataatcgcag
    11761 aaacgttatg aggtttgtgg acctgctcca tgcagacggt cagatcaggc tgccgcctgg
    11821 cgagcatttc gcagatgcgt gcataacgtg ttgggttcat aaaacattaa tttcggtttc
    11881 gggtgacttt aatcacgtct ggcatcacgc ggattttgcg catgatattc gccagatgca
    11941 cacggtcacg agcggtcaga cgaataaagg cgctgtagac gcgaccatct ttctcttccg
    12001 tattcaaact ttgaatattc gaagtcgtgg tgttaattgc cgccgtcagg tttgccagcg
    12061 cgccctgatg attgaacatc tccaccttga tttcggtgat gaactcctgc gccgtctctt
    12121 tatcccattc cacagccata aacttctctg gctctttctg gtagccacgg atattacggc
    12181 aggattcatg gtggatcacc agacctttac cggggctgac gtgggcgata atcgggtcgc
    12241 caggaatagg gcggcagcat ttcgcaaagg tgatcagcac gccatcggca cctttaatgg
    12301 gcagatgtcc gtggctttgg gttgccggtg gaatggaggc gtccccatgt tgcagatttt
    12361 tcgcgaccac cacgctcatt gcgttaccaa gtccgatttc tgccagcaga tcgtcaagcg
    12421 ttgccagctt catgcgatcc agctcgcgct gaatattttc ctgcgggatt tcattcagct
    12481 tacggctacc acccaaagca tggttgagca gacgacggcc caggcttaca gaatcatcac
    12541 gcttgaggtt tttcagcaac tgacgaattt tggcgcgcgc ttttgagcta acgacaaagt
    12601 tcagccacgc ggcattcggg cgagcgcccg gtgcggtaat gatttcaacg gtttgaccgc
    12661 tggtaagcgg ctgcgacagc gggtaaggct ggcggtcaac gcgtgcgccc acgcaggcat
    12721 gaccgatatc ggtatgcact gcataagcga agtcgacggg cgttgcaccg gcaggcagct
    12781 cgacaattcg cccttccggt gtgaaaacgt aaatctcatc cgggaagaga tcggatttaa
    12841 cgctctcgat aaattcaaac gaactaccgg cgctctgttg cagctccagc aggctttgca
    12901 tccagcgctg ggcgcggatt tgcgcggtgg tgctggtttc gccgtgctct ttataagccc
    12961 agtgcgcggc aacacccatc tccgccatct gatccatatc ttcggtacgg atctggacct
    13021 caaccggcac accgtgcggg ccgatcatcg aggtgtgcaa agactgatag ccgttcgctt
    13081 ttggaatggc gatgtagtct ttcacgcgac ccggacgcgg cttgtacagg ctatgcattt
    13141 gcccgagcac gcgataacag gtgtcggaat cattgacgat cacgcggaaa gcgtagatgt
    13201 ccatgattga gtgaaaacgc tgctctttga gcaccatttt gcagtaaatc gaataaagat
    13261 gcttctcgcg accactgacg cggcagggaa ttcccgcttc ctgcaaacgc ccttcgattt
    13321 ccgagaggat tttttggatc atctctttac ggttgccgcg cgcggctttc accacttctt
    13381 tgattacgcg ataacggttg ggatacagcg cctcaaaacc cagctcttcg agttcggttt
    13441 taatgtggtg gatacctaaa cggtgcgcca gcgggctgta aatttcgaga gtttcacggg
    13501 cgatgcggcg acgtttgtcc gggcgaagtg agcccagcgt gcgcatgttg tgggtacggt
    13561 cggcaagttt gatgaggatg acgcggatat cctgcaccat cgccataatc atcttgcgaa
    13621 agttttcggc ctgcgcctct ttcttatcgc ggaacttgag tttatcaagt ttcgacaccc
    13681 cctctaccag ctcggcgacg cttttaccaa aaagctgttc catatcctgg taggtggcgg
    13741 gagtatcttc aatcacgtca tgcagcagcg ccgccatcag cgtttcatag tcgagtttca
    13801 tctcggccag aatgcaggca accgctaccg ggtgcgtgat atagggttca ccgcttgaac
    13861 gtgtttgccc ctcgtgagca tcacgtgcaa cgagatacgc ctgccgcaga cgcttgattt
    13921 ggtcttccgg caggtaggtt tgaatcagtt gattcaggct ttcaaacaga tacaagggcg
    13981 acccgctttg tgattaacga cgaccttcag caatagcggt tacggcttgt aattcagcgg
    14041 attcctgctc ttgctgttcc tggcgttcgc gaacgtcgag gatctggttg ttgatcagac
    14101 cttcttcgat ttcgcgcagc gcgattacag tggttttatc gttttcttcc ggtaccagcg
    14161 gatcctttcc gcctacctgc atctgacgag cgcgacgcgc ggcgaccagt accaggtcaa
    14221 aacggttacc aattttctct acagcgtcct gaacagttac gcgtgccata cttaaaaagc
    14281 tccacaggtg aagaaatgac tgggcatgat actgaaatca ggttcagtct gccaacaatt
    14341 tgctgattaa agcgtcatga cgctgctttt ggcggctcat gcgcagacgt tcggcgcgaa
    14401 taatggtctt caaatcggtc aacgcggtat cgaagtcatc attcacaatc agataatcat
    14461 attcggcgta atggctcatt tctgcaacag cttgcgccat acgctttgca atgacctctt
    14521 cgctgtcctg accgcgaccg cgtagacggc ggtccagttc aattttggac ggcggtaaaa
    14581 taaagatact ccgcgcgtgc ggcatcttct ggcgaatttg ctgcgcgccc tgccagtcga
    14641 tatcgagaaa aacatcgaca ccggtcgcca gtacttgctc aatggcctca cgcgaagtgc
    14701 catagtaatt accaaaaact tctgcgtgtt cgaggaacgc atctctgcta atcatttctt
    14761 taaattcatc atgattaaca aagaaataat gttcaccgtg gacttcacca ggacgcggtt
    14821 ggcgtgtggt gtgtgaaaca gaaacctggg tgtcatacaa cggttgggtt tttaataaag
    14881 cctgaatcag gctggattta cccgcgccac tgggggcaga aacaatataa agcgtgcctt
    14941 gagccatgag tatctttcgt atgtgattag cgaaataaag cctacatacg agcttattat
    15001 acacggcgcg gccatgtgac gtagcccttg ctcacacttt ttgcgcaaag ttgttgcgtt
    15061 ttgcgtagct gtttccgcat ttgcctgtga tttactgcaa taaagacgat tctcaggaag
    15121 cgtcctcgca gactccgttt ttgccactcg gctttgcctt caccacgttg tatatctgcc
    15181 agcctgacta cagcggaggt gaagatgaaa gtatggatgg cgatattaat aagtatcttg
    15241 tgctggcaat catctgtgtg ggcggtctgt ccggcctggt cgccagccag agcacaggaa
    15301 gaaatttccc gcctgcaaca gcaaataaaa cagtgggacg atgactactg gaaggaagga
    15361 aaaagtgagg tggaagacgg tgtttacgat cagttaagcg cccgtcttac gcagtggcaa
    15421 cgctgctttg tgagcgagcc ccgcgacgtg atgatgccgc ctttaaatgg tgcggttatg
    15481 catccggttg cccatacggg tgtacgcaaa atggcggata aaaacgcatt aagtctgtgg
    15541 atgcgagaac gtagcgatct ttgggtgcag ccaaaagttg atggcgttgc ggtaaccctg
    15601 gtttatcggg acgggaaact gaacaaagca atcagtcgcg gtaatggcct gaaaggcgag
    15661 gactggacgc agaaagttag cttaatttcc gctgtgccgc aaaccgttag cgggccttta
    15721 gccaacagta cgcttcaggg ggaaatattt ctccagcgcg aggggcatat ccaacaacaa
    15781 atggggggaa taaatgcccg cgcaaaagtt gctggcctga tgatgcgcca ggggaatagc
    15841 gacacgctaa actcactggc tgtttttgtc tgggcatggc cggatggacc gcagttaatg
    15901 accgatcgtt taaaggagct ggctaccgca ggttttactc taacgcagag gtatacccgt
    15961 gcggttaaaa atgctgatga ggttgcgcgc gtacgcaatg agtggtggaa agcgaaatta
    16021 cccttcgtca ccgatggcgt ggttgtgcga ggggctaagg aacccgaatc ccgtcattgg
    16081 ctaccgggcc aggcggagtg gctggtggcc tggaaatatc aacctgtagc tcaggttgcc
    16141 gaagtgaagg cgattcaatt tgcggtgggt aagagcggta aaatatcggt ggttgcgtca
    16201 ctcgcacctg tcatgctgga tgataaaaaa gtccagcggg tgaatattgg ttccgtcagg
    16261 cgctggcagg agtgggatat tgcgcctggt gatcagatcc tcgtcagcct tgccggtcag
    16321 ggtattcctc gcattgatga tgtggtctgg cgcggtgcag aacgtacaaa accgacacca
    16381 ccagaaaacc gctttaactc gttgacctgc tactttgctt ctgatgtttg tcaggaacag
    16441 ttcatttcac gcttagtctg gctggggtca aaacaggttc tggggctgga tggcattggt
    16501 gaggccggtt ggcgcgcgct gcatcagact catcgctttg agcatatctt ttcctggctt
    16561 ttattaacgc cagagcaatt acagaacacg ccggggatcg cgaaaagtaa aagtgcgcag
    16621 ttatggcatc agtttaatct ggctcgtaac cagcctttta ctcgctgggt gatggcaatg
    16681 ggaataccgc taacccgggc ggcgcttaat gccagtgatg aacggtcctg gtcgcaactt
    16741 ttatttagca cggagcagtt ctggcagcag ctgccgggga ctggatctgg gcgcgccaga
    16801 caggttattg aatggaagga aaatgcgcaa atcaagaagc tgggcagttg gctggctgcc
    16861 cagcagatca caggttttga accttagtga ccttcgtggc tgtagtaaaa gaccggtaat
    16921 cccagcttaa gacgtagcgc cagtaaacga gcaaaaaagc cgaatactag ggtggagata
    16981 atcaccacat catggttaga aacatagtgt tgcagcgcga tgtacagcac ggcggaggca
    17041 aatgagacac cggcatacaa ctctttttga aagaccagcg gaatgcgttt acagaacata
    17101 tcgcgcaaaa cgccgccaaa tacaccggtg gttaccgccg cgacaacggc aataattggg
    17161 ccgtgcccca tatccagtgc aacctgtgcg ccgatgatag aaaagaccac cagtccgagc
    17221 gcatcgagca ccagaaacac tttgcgcagg taaggcatta caggagcaac gatagtggtg
    17281 agtactgcgg cggtagcgac gataatcaca tattccgggt gtttgaccca gccgagcgga
    17341 tagtggccca gcagaatatc gcgcactgac cctccgccaa ttgcggtggc ggttgcaata
    17401 ataattacgc caaatgtatc catgcgccgt cgcccggccg ccagcgcccc cgtcatggct
    17461 tcggcagtaa tgccaaccaa atacaaaatg tgtaacagca tggttctctc cctaaaaatc
    17521 tgtctggcag ggtagcgatt tgtgcgcatt gtcacgattg atatttttta aggtgagagg
    17581 aaattgataa cttttagtta tgtgaaatcg ctattttctg tagcgtagat gcattcttct
    17641 gacttcttgt ctgagaaaat gcataagaaa aaataggtta aaactgaggg gggaaaagaa
    17701 aggtgcaatg tttagccatt gtgttcactg cacctggacc ttggcttacg catcgggtgt
    17761 ctcaatcaac aaaaagagtt tttcgtttct gctatttctt ctctgtaatt tttacactgt
    17821 tttccaactg cttgatgctg acctgcgggg tcggcaactc ctcaggcatg gttccgccaa
    17881 gttcctgaat ggtttgcctc accttgctac ccacgtcaaa gtgtgtggta ttagcttgct
    17941 gttttgaatt cacgctatcc cgcttgagtt tttcttctgt ttgggtagct cgaaagagat
    18001 tagccgccag ttctgttgaa cccatatgat ccaggatctt ctgactcttt ttcagctcct
    18061 tcagttggtg gatggctttc tgatctaatc caccatacag cccctggtaa ccatgattct
    18121 gaaatatggc gaaatcagta gctgtcgcta ccccagcttg ctgtgctgcc tcaaccaatt
    18181 gtttgttatg ttctttcaac tcatttctca gaaacagacg cttttcgtct tcacgaagtt
    18241 gcttaaatgc ttcatcgtca gcaagctcct gccgtcgggt ctggatagta aaataagtct
    18301 gccctgccgc aatgaccggt ttcgcggggt cgccgttttg tactaccagg taacaggcat
    18361 agcgggagag tacgatgtct tttaatgctc ttttggcatt tgagccaatt ttgaccatat
    18421 cgaggatctc ctcgaaatgg tcatcaattg tgtgaccact gttctcacag gcctctttgg
    18481 cgcgcgttaa tacaggtata aagtgacgat actctgaata acccagtagt ttcccaagtt
    18541 gtcttgctga ccattgttct gctc
//
LOCUS       DADLSB010000075        18528 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.075, whole genome shotgun sequence.
ACCESSION   DADLSB010000075 DADLSB010000000
VERSION     DADLSB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.075"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..419)
                     /locus_tag="JN331_002421"
     CDS             complement(<1..419)
                     /locus_tag="JN331_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7260546.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLG"
     gene            526..822
                     /locus_tag="JN331_002422"
     CDS             526..822
                     /locus_tag="JN331_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7260547.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(956..1381)
                     /gene="arsC"
                     /locus_tag="JN331_002423"
     CDS             complement(956..1381)
                     /gene="arsC"
                     /locus_tag="JN331_002423"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY7260548.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1394..2683)
                     /gene="arsB"
                     /locus_tag="JN331_002424"
     CDS             complement(1394..2683)
                     /gene="arsB"
                     /locus_tag="JN331_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY7260549.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLSMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2737..3090)
                     /gene="arsR"
                     /locus_tag="JN331_002425"
     CDS             complement(2737..3090)
                     /gene="arsR"
                     /locus_tag="JN331_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY7260550.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            3833..3916
                     /locus_tag="JN331_002426"
     CDS             3833..3916
                     /locus_tag="JN331_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY7260551.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3970..5322)
                     /gene="gorA"
                     /locus_tag="JN331_002427"
     CDS             complement(3970..5322)
                     /gene="gorA"
                     /locus_tag="JN331_002427"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY7260552.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5394..6236)
                     /locus_tag="JN331_002428"
     CDS             complement(5394..6236)
                     /locus_tag="JN331_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY7260553.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6439..8481
                     /gene="prlC"
                     /locus_tag="JN331_002429"
     CDS             6439..8481
                     /gene="prlC"
                     /locus_tag="JN331_002429"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY7260554.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8489..9241
                     /gene="rsmJ"
                     /locus_tag="JN331_002430"
     CDS             8489..9241
                     /gene="rsmJ"
                     /locus_tag="JN331_002430"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY7260555.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9290..10759)
                     /gene="dtpB"
                     /locus_tag="JN331_002431"
     CDS             complement(9290..10759)
                     /gene="dtpB"
                     /locus_tag="JN331_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HAY7260556.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11077..11511)
                     /gene="uspA"
                     /locus_tag="JN331_002432"
     CDS             complement(11077..11511)
                     /gene="uspA"
                     /locus_tag="JN331_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY7260557.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11902..12237
                     /gene="uspB"
                     /locus_tag="JN331_002433"
     CDS             11902..12237
                     /gene="uspB"
                     /locus_tag="JN331_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY7260558.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12308..13807)
                     /gene="pitA"
                     /locus_tag="JN331_002434"
     CDS             complement(12308..13807)
                     /gene="pitA"
                     /locus_tag="JN331_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY7260559.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14039..15238
                     /locus_tag="JN331_002435"
     CDS             14039..15238
                     /locus_tag="JN331_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7260560.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKIPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSQKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
     gene            16404..17915
                     /locus_tag="JN331_002436"
     CDS             16404..17915
                     /locus_tag="JN331_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HAY7260561.1"
                     /translation="MPLQQFSRLALTLAIGLSIAAGGAALTWYQVAETDLSRTAAAAH
                     EAVSVTERLLDEAQGAIDLVYPLSVQPCTPQLRTELGRLTISHEHIRVINIFNQNLLS
                     CSSWEGADPVREFISAGDNQTLILATDDYISPGVPVMILRKTDNDNAITASIATKWVA
                     DNMTLLNLRRPLSLRVGNTVLTSDNTLVKAIPGGNRHAVYSGLYPFSIEYPGDLSVPL
                     TLYFHDGALSLLLSVLLGAAVATGLWKIKFRRKSRYDEIYEAMRKDEIVPWYQPIMDA
                     STGEIAGVEVLARWVKADGEVIYPGSFITEVERSDLVIPLTRSLMARASRDLLPLTGD
                     GKRWHISFNITQSHIMETGFIAECLTFIDAFTPGSITLTIELTEREPFDNSDEMRCQL
                     KRIHDAGIALALDDFGTGYANMEYLSEIPVDIIKIDRVFVNRIGQGESAEHLLTSLIE
                     MANTLKKEIIAEGVETEAQAAWLYERGVGKLQGFLYSPALPPDKLALFKPKMS"
BASE COUNT         4680 a         4576 c         4786 g         4486 t
ORIGIN      
        1 cccagagaat ctaggataat actggcaaga acggagacgc cggctgcgat aatcgcgtac
       61 aacaaggtga ctttccaacc aaaggtcatc cacattagtc cgacgataat gggattaagt
      121 aatggtgaaa caaataaaaa agtgagagtc ggaccaaacc cggctttcgc tgacaacaat
      181 ccacgtaaca tgggtattgt cgagcaacta cagaacgggg taacggctcc caacagagca
      241 gccaggagat agcctctccc ttttctggcc cccatcatct gctggatttt atggtctggc
      301 accttttgtc ttatcaggct gacaccggca ctaatcacta taaacagcaa cgaaagctcg
      361 acggcgagaa acacaaacat ctctgcggca tcttgcagca tcgcaagcca actactcata
      421 cgtaacctca ttggataaat ctatatttcc agaataatcg aaatgaaatt cagatcaaga
      481 tatttctggt attatcgaaa tatcaaccag acagaggggt gtcatatgca gttggaagaa
      541 gtcgctaaag cgctgaaaga gctaggtcac ccgacccgtt tgttcatatt taaacatctt
      601 gtcaaagccg gggaacaagg gttgccggtc ggtgagttgc aaaagcagct tgggatacct
      661 ggctccacac tcagccacca tatatcagca ctggtatcgg taggactggt caaacaaaac
      721 cgtgagagtc ggacgctgat gtgtgtttcg caatacgcaa tgctggaagc gataattgaa
      781 ttcttacgcg aggagtgctg cgtaaatagc aaaattgcct gatgcgctac gctctacata
      841 tttcatgcca tatattgaat ttgcacggtt ttgtaggtcg gataagacgc gttagcgtcg
      901 catccgacat aaacaaagcg cactttacca actatcggca gtggatatcg ccgctttatt
      961 tcaggcgctt acccgcttca tcaaccactt tctcgccatc ttccttggag aatgcgcctt
     1021 tttgcgcatc tggcagaatt tccagcacca cttctgaagg gcggcacagg cgagttccca
     1081 gcggcgtcac cacaatcggg cgattaatca gaatcgggtg ctgaagcata aagtcgatta
     1141 accgatcgtc agtaaattta tcttccgcaa ggcccagctc ctcatacggt tcgacgtttt
     1201 tacgcagcag cgcgcgtacg gaaatcccca tatcggcaat gagtttgacc agttcatcgc
     1261 gcgttggcgg agtttccaga taatggataa tggtcggttc tgtgccgctg ttgcggatca
     1321 tctccagcgt attacgcgac gtgccgcagg ccgggttgtg ataaatggta atgttgctca
     1381 tatcagtatc tcattacaaa gtgaaagaga gacgtagcgc cagcgcagcc agcgtcacaa
     1441 acagcacagg cagggtcatg ataatccctg tacggaagta atatccccag ctgatagtca
     1501 tattcttctg cgaaagtacg tgcagccaga gcagcgttgc caggctacca atcggggtaa
     1561 ttttcggccc caaatcgcag ccaatcacgt tggcataaac catcgcttct ttgataacgc
     1621 cagatgccgt gctgccatca atggacaacg cgccaaccag taccgtcggc atattgttca
     1681 taatagaaga gaggaaggcg gtgaggaatc cggtgccgag cgtcgcggcc cacaggccgt
     1741 tatccgccag cacgttgagt acgccagaaa gatattccgt taatccggca ttgcgcaggc
     1801 cataaaccac cagatacatg ctgagcgaga agatgacaat ctgccagggg gcaccgcgca
     1861 ggactttacc cgtattaatc gcatgaccgc gtttagcgac gacaaataat atcagtgcgc
     1921 ccacagctgc aatggcgctc accggaatgc cgagcggttc caagacgaaa aatcccacca
     1981 gcagaagcag taaaacaacc cagccagttt tgaacgtagc aggatctttg atcgcttctg
     2041 cgggagattt cagcagcgcc atatcgtagt tctgcggaat atctttgcga aaatagagat
     2101 gtagcatcac cagcgtggca acaatcgcgg cgatatccac cggcaccatc accgaggcgt
     2161 attcgcgaaa gccgaggcca aagaaatcag cggaaacgat attcaccagg ttggagacaa
     2221 taagcggcag gctggcggta tcggcaatga atccggccgc catcacgaac gccagcgtcg
     2281 tgcctttact gaatcctaaa gccagcagca tggcgatgac aatcggcgtc aaaataagcg
     2341 ccgcgccatc attggcaaac agggccgcaa cggcggcacc gagcaggaca atccaggtaa
     2401 acagcaagcg accgcgtcca ttgccccagc gtgaaacgtg cagcgctgcc cattcaaaaa
     2461 agccggactc atccagcagc aggctgatga taatgacggc aataaacgcc gccgtcgcgt
     2521 tccagacgat attccacacc accggaatat cacccggatg aaccacgccc gtaaccaacg
     2581 ccagtaccgc gccgagcgtt gcactccagc cgatgcctaa acctttcggc tgccagataa
     2641 ccaatacgat ggtcaggaca aagatagcgc ctgccagtaa cataatgcct cccggataaa
     2701 acacatgtgc aaatccatat gtgtttagct aaatttttaa ctgcaaatgt tcttactgtc
     2761 tccggaacag ttttgtctgg ccaggttgcg gacaattgcc tgaatctttt cctgttcaca
     2821 tcgccaggct tcatcaataa ttttcgccgc ccatgctgga atatgcggcg ataagcggta
     2881 atgaacccac ttaccttgct tgcggtccag caatagcccg ctttcacgca gcaatgccag
     2941 atggcgggat atcttgggct gcgactgatc gagggcagtg cagagatcgc agacgcataa
     3001 ctctcccagt tcgctaagca gtaaaacgat gcccagacgg gtttcatcag caagaatttt
     3061 gaacaattgg atgggtaaca gaaatgacat attgcgctcc tgattgttgc aggtagtgtc
     3121 tctcttcgaa gcggataagt caaaaacata tatgacttaa cgaatgtgta agtgcagagg
     3181 aaggtaatag gtgtgaattt tgagttggct attcatttga aaggcggttt gaatcaggac
     3241 gtaaaaaaac cgactttgcg tcggtttttt actcttcagc ctgagttggt ggctctggct
     3301 ggagtgagaa tgcccacatc taacggttca atactattag ccagtggcta acattgcaag
     3361 cgttagcgat ttcttcagct ggagaaggga cagtttgcta tacatggtgg tgaaagagga
     3421 tttatatact ttcatcgggt cgctgccgta ccaagccttg tccgaatccg gacgtaaaaa
     3481 aaccgacttc gcgtcggttt ttttactctt cagcccagag ttggtggctc tggctggagt
     3541 gagaatgcca tcatctaacg catcaatgct aataccatac tgaaactatt gcaaggttat
     3601 gctggttatt tagcctaatt acactgtatg attatccagt tagctctgag gcattttcac
     3661 tctggcaatg cgcataaacg ctttcaaagt cctggtcaga agtacgggtg gtgccgttaa
     3721 ctgatgctct ggccggagtg agattgccca catctaacaa tgaggcatgc gccgtgacag
     3781 gcagtggatg agtaagcgga tgcattctca ctccatcgca tggagaaaac gggtgattga
     3841 taaagcaatc atcgttcttg gggcgttaat tgcgctgctg gaactgatcc gctttctgct
     3901 tcagcttctg aactgatagc ggaaacgtaa ttaagggcta agagcacact actcttagcc
     3961 ctttaacatt taacgcatgg tcacgaactc ttctgccgcc gttgggtgaa tggcgacggt
     4021 attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc accgcgaagc cctgcaacat
     4081 ttcgtccata ccaaagccaa tgccgtgaat accgacaatc ttctcttctg gcccaacgca
     4141 caccagcttc atgcggcacg gctggcggtg agtggtgacg gcggtataca tcgcggtgaa
     4201 agaggattta tacactttca cctgatcgtc gccgtactgc tcgcgcgcct gcggttccgt
     4261 taaaccaaca gtaccaatcg gcggatggct gaagaccacg gtcggaatgt tgctgtaatc
     4321 cagatgctca tccggcttgt tattaaacag gcgttcagag agacggcgac ccgctgcaac
     4381 tgccaccggt gtcagttcca ctgcacccgt gttatcgccc accgcgtaaa taccttcaat
     4441 attggtgttt tgatatttat cgacgacgat atagcctttt tcgttggttt taacgccagc
     4501 ggcttcgagg ttgatgttgt cattggcagg ctcgcgacca atcgcccaaa tcaggcaatc
     4561 caccgtttca ctgcgaccat cttccagctc cagcgtcagg ctaccatcgg catttttcac
     4621 taccgctttc gggatggcgt tggtatgcaa ctgtgggcct tcggcgttca tcacttcgac
     4681 cagcgtttca gagatcatcg ggtcgaagct gcgcagcggc gcatgtttac gcacaaacag
     4741 atgcgttttc gcgccgaggc cgttaatcac gccagccagc tcaacggcga tgtaacccgc
     4801 gccaacaacc gccacgcgct ctggcaaagc aggaagggcg aagaagccat cggaatcaat
     4861 accgtattcc acgcctggaa tatccgggtg gctcggacga ccgccggtgg cgatcagaat
     4921 atgatcggcc gtgatggttt cgccgttaac ttccagcgtt ttggcatcaa cgaagcgggc
     4981 aaagcctttg attacatcaa cgttattttt accgagcacg ttttcatagg aagtatgaat
     5041 acggtcgata taggcggtac ggctggcgat caacgtttcc cagttgaact tgttgatggt
     5101 ggtatcgaaa ccataatcag ggccgtacat atggatcgct tcacggattt gcgccgcgtg
     5161 ccacatcact tttttcggca cacagccgac gttcacgcag gtgccgccca gctctttggc
     5221 ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg ttgatggagg cgataccgcc
     5281 gctgccgccg ccgatggcga tgtaatcata gtgtttagtc atgacatatt gtccttatcg
     5341 ttgattaccg cgattgtagc gcgagacgta ataggtgcca gcaatggctg caattactcc
     5401 ggcacgatcc agcttacggt ggcgtgcccg gtacctgccg gaaccagttt gctgtgcagc
     5461 cacgacagca cgttattcat ctgctgttcc agcttccacg gcgggttaat cacaatcatg
     5521 ccggaagcgg tcatgccacg gcgatcgctg tctggcagca ccgccagttc aatttgcaga
     5581 attttgcgaa tgccggtcgc ttccaggtcg tggatcatgc gcttaatttg ctggcgcagc
     5641 accaccggat accacaatgc gtaagtgcca gtggcgaaac gtttgtaacc ttctgctatc
     5701 ccgctgacca ccgcctgata gtcggttttc atttcatacg gcgggtcgat aaggattaaa
     5761 ccacggcgag aaaccggcgg cagtttggcc ttaagttgct ggaaaccgtc ggctttttcg
     5821 acgcgtgcac ggctgtcttt ctgaaatcca gaacgcagca acgggtaatc gctcgggtgc
     5881 agttcggtca gttgcaggct gtcttgttcg cgcagtagct ggcgagcaat caacggcgaa
     5941 ccggggtagt aacgcaactg gccgctacgg ttgaagtgtt ttaccacatt gatgtacgcc
     6001 tccagttctg cgggcaaatc gtcctgctgc cagatacggg cgataccttc gagatattcg
     6061 ccggtacgct cggcatgttc gctacctaac tgataacgcc cggcccctgc gtgggtgtcg
     6121 agatagagaa acggtttatc tttctctttc agcgactcga tgatcaggct ctgaacggta
     6181 tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt ggcgataact gagcatgggt
     6241 aaaggtgttc cggtaagtaa aaattggccc gtattcgggg cgcacaaaag cctatcagga
     6301 cagtataccg aaaagaggcc gccgccgcga aagcgtaacg tttctcattg aaattcacta
     6361 cacttaaccc catgctacat acattatgta aagcgcctgt tgagcgcttc cttaacctct
     6421 ttaaccagga ctgcgctaat gacgaatccg ttactgactc cctttgaatt gcctccgttt
     6481 tctaaaattc tcccggaaca tgtcgttcca gccgtgacta aggcattgaa cgactgccgc
     6541 gaaaatgtgg agcgcgtagt agcgcaaggg gcaccgtaca cctgggaaaa tctctgccag
     6601 ccgttggcgg aagtggacga tgttttgggg cgtatcttct ccccggtcag ccacctgaac
     6661 tcggtgaaaa atagcccgga actgcgtgaa gcgtacgaac aaaccctgcc gctgctgtcg
     6721 gaatacagca cctgggtagg gcaacatgaa gggctgtata aagcgtaccg cgacctgcgc
     6781 gatggcgatc attacgccac gctgaacacg gcgcagaaga aagcggttga taacgccctg
     6841 cgtgattttg aactctctgg cataggtctg ccgaaagaga aacagcagcg ttacggcgaa
     6901 attgctaccc gtctttctga actgggcaac cagtacagca acaacgtcct cgatgcgacg
     6961 atgggctgga ccaaactcgt taccgacgaa gcggggctgg cggggatgcc agaaagcgcg
     7021 ctggctgcgg caaaagccca ggccgaagcg aaagagctgg aaggctacct gctgacgctg
     7081 gatatcccaa gctacctgcc ggtaatgacc tactgcgaca accaggctct gcgcgaagag
     7141 atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc cgaacgccgg taagtgggat
     7201 aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg aactggcgca actgctgggc
     7261 tttgaaaact acgcctttaa atcccttgcc actaaaatgg cagaaaaccc gcagcaggtg
     7321 ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc aaggcgaaaa agagctggag
     7381 caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg agttgcagcc gtgggatatc
     7441 gcttactaca gcgaaaaaca gaaacagcac ctctacagca tcagcgacga gcagctgcgt
     7501 ccgtacttcc cggaaaacaa agcggttaac ggcctgtttg aagtggtgaa acgtatttac
     7561 ggcatcaccg ctaaagagcg taaagatgtt gatgtctggc atccggatgt acgtttcttc
     7621 gaactgtatg acgaaaataa cgaactgcgc ggcagcttct acctcgatct gtatgcccgt
     7681 gaaaacaagc gcggcggggc gtggatggat ggctgcgtag gccagatgcg taaagctgat
     7741 ggttctctgc aaaaaccggt cgcgtatttg acttgtaact tcaaccgccc ggtaaatggt
     7801 aaaccggcgc tgttcactca cgacgaagtg atcaccctgt tccacgagtt cggtcacggc
     7861 ctgcaccata tgctgacccg catcgaaacc gctggtgttt ccggtatcag cggtgtgccg
     7921 tgggatgcgg tcgaactgcc gagtcagttt atggaaaact ggtgctggga gccggaggcg
     7981 ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc tgccgaaaga gttgctggat
     8041 aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta ttctgcgtca gctggagttc
     8101 ggcctgtttg atttccgcct tcatgccgag ttccgcccgg atcagggggc aaaaatcctc
     8161 gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc catctccgtc ctggggccgt
     8221 ttcccgcacg ctttcagcca tattttcgcc ggtggttatg cggcaggtta ctacagctac
     8281 ctgtgggctg acgtactggc ggcagatgct ttctcgcgct ttgaggaaga gggcattttc
     8341 aaccgtgaaa ccgggcagtc gttcctcgac aacattctga gccgtggcgg ttcagaagag
     8401 ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc agctggatgc gatgctggag
     8461 cattacggca ttaagggctg atcattcagt gaaaatctgc ttaattgatg aaacaggcgc
     8521 cggagacggc gccttatctg ttctggcggc ccgctggggg ctggagcacg atgaagacaa
     8581 cctgatggcg ctggtgttaa cgccggaaca tctggaattg cgcaagcgtg atgagccaaa
     8641 acttggcggc atctttgttg attttgttgg cggagcgatg gcgcaccgac gcaaattcgg
     8701 cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt aaaggcgatt atttgccgga
     8761 tgtggtggac gccaccgcag ggctggggcg cgatgccttt gtactggctt cagtcggctg
     8821 ccgcgtgcgg atgctggagc gtaatccagt ggttgccgcg ctactcgacg acggcctggc
     8881 gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag gagcggttgc agttaattca
     8941 cgcctccagc ctgacggcgc taaccgatat taccccacgc ccgcaggtgg tctatctcga
     9001 tccgatgttc ccgcataagc agaaaagcgc gctggtgaaa aaagagatgc gtgtctttca
     9061 gtcgctggtg gggccggatc ttgatgccga tggattactg gagcctgcac gcttgctggc
     9121 gaccaaacgc gtggtggtaa agcgcccgga ctacgcgcca ccgctggcga atgtcgccac
     9181 gccaaacgcg gttgtgacta aagggcatcg ctttgatatc tatgcaggta cgccagtgta
     9241 aaagtaaagc agggtagcga taacgctacc ctgctttctg caagaataat taatggcttt
     9301 ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa cagcatcact actgcaacgc
     9361 ccagcgtgac cagaccaatc ttaccaaaca cgttggtata gacgggcaac gtctcaagcg
     9421 gatcggtaat gttgtccggc accgcggtaa atgttgccac atagccgccc agcaagaacg
     9481 cggcagcctg cgtcaggaac cacatcccga gaataaagcc catcaaatgc tgcggcacca
     9541 gggcagcaat catcgccagg ccaagggcgc taataaacaa ttcacccagg ctctggaata
     9601 aatacaccag cacgataaac catggcgatg tcagcccttg ggcatccgca aaccacattc
     9661 ccgcagcagc agccgtcaaa aagcccagtg agcacataaa catgccgaga gtaaatttca
     9721 tcggcatcga gaggtctttg cctttgttac ccagatgcgt gtaaatgcct gccagtattg
     9781 ggctggcgag taccacccag aacgggttaa gcgcctggaa gctgaccggg ttgatggaaa
     9841 aaccgagaat ttcatgatgc acgttgttga tggcaaagaa gttcagcgat gttggcatct
     9901 gggcgtagag aatgtaaaac accaccgctt cgagcatcag gacaaaggcg acaaacattt
     9961 tattgcgccc ggttttatcc agcttgaatg cctgacgaaa gaagatgatg gtgacgacga
    10021 tggagagaac aatcagcacc agattggcga cttctacgtt gtgcatcagc catgcacata
    10081 cgaagatcat caccacgctg ccaagtaata cgtacaacag tttgctgaag ctcatcggct
    10141 tgaagtcggg ttcagaacca atgtctttca ccattccacg acaggcgatg taaaccagta
    10201 atgcgataat taaccccgcc ccgcacaggt tgtaggtgac tgaataaccg aatctatcag
    10261 cgatcacagg ggccagcgat aacgctatca acgagccgat attgatcgac atatagaaca
    10321 gggtgaatgc gccatcaagc cgcggatctt tcggcggata gcacttcgaa agcaagctgg
    10381 ctgggttagc tttaaacagg ccgttaccga cagcgatagt ccccagggcg atgaaaatca
    10441 ggtcaggctt aagtagcgac atgccggtca tgaagtagcc aatcgccagc acaagtgctc
    10501 caagaacaat ggtgcgtttg gtccccaaca ggtggtcgcc gacatagccg ccaatggaaa
    10561 tgaggccata gaccagtgca gcaaaagcac caaaagtgac aaaagcctgc tcttgcgaga
    10621 atccaagctg tttaacgaag aaaaccgcca gtacgccctg cacgccgtag tagccgaatc
    10681 gctcccataa ctcgacaaaa aagatcatga aaaatgggcg aggttgctgc agcatcccca
    10741 tgggtgttgt tgtattaata tttattaacc ttccaatacc atcctgaaaa gtatgaacgc
    10801 gtaagggata acgcgcgaaa aagtttacat ctgtcagaga ttcttaatat tgatgcggtg
    10861 aatagattaa tacactatta caacagaaaa taaccagctg atcacactaa tgatgggaaa
    10921 tatgatggtg ttaaattttg tattggcagg agattttgat tgcgtacgca gatcgtaggc
    10981 cagataaggg gtttacgctg catcgggtaa caaggcgcgc caaaaagcaa aacgcccgca
    11041 tatgcgggcg ttcaggaaca cgtcgtggag ggatgattat tcttcttcgt cgcgcagcgg
    11101 aacaatcagc atatcaacgt gaacggtgtt gatcagctga cgtgcggaag acatcagttt
    11161 gctccagaag tcctggtggt gaccacaaac caccagatcc atatcgtatt tcttgattgc
    11221 atcgaccaga acctggccca ggtcgccgct gccgctcagg gtttcagtga ttggatagcc
    11281 tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct tcagagatgc gtttctgcat
    11341 atcacccaga ttcacatcaa taagcccggt gtataggtca gagtagttta catctacgtg
    11401 gatcagagaa actttcgcat tgtaggggcg agccatagag actgctttct ctaccagaac
    11461 tttgctttcc ggggagagat cgactgcgat gagaatgtgt ttataagcca tagtgttact
    11521 ccttccataa agttgtcgat gactggccag ctagcgtttc ttgtgccgtt acgtcacccg
    11581 cgtcctgcga actacgtgcg cggggctaac ccagccgtaa agattcagtg ttaagaccat
    11641 ccttacctta tagcgacccg gatgatccgt caatccgcct tgcttacccg ttagataaac
    11701 aaattttcga catgaatata ttgatagtgg ttaaccttct ggaaaaaaaa caacctgatc
    11761 tcctacacta tctatagagc cgctcggatg ttggtcacca caaaagcggt ttctccagga
    11821 cctggtgtta actgattggc agcgtatcgg gagcggtagc cccggggtgg aactccgcgg
    11881 gcaggtcgcc ggggaggagg tatgataagc accgtcgcat tattttgggc tttatgtgtc
    11941 gtttgcattg ttaacatggc gcgctatttc tcatcactac gcgcgttgtt agtggtactg
    12001 cgtaactgcg atccattgct ctatcaatat gttgatggag ggggcttttt tacctcacat
    12061 ggccaaccca acaaacaggt gcgtctcgtt tggtatatct atgcccaacg ttatcgcgat
    12121 catcacgatg atgagtttat tcgccgctgt gagcgggtgc gtcggcagtt tattctgact
    12181 agcgcattgt gtggtctggt ggtggtcagc ctgattgcat tgatgatttg gcattaagca
    12241 cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa ctgacccgct cgttttggtg
    12301 cgtacgatta caggaactgc aaggagagcc agtacagccc gccggaaagt agtaccgcag
    12361 ccggaagggt aaacacccag gccatcagaa tactggttac ggttttacgc tgtaagccgc
    12421 cgccatctac caccatcgtc cccgcgacag aagaggagag tacgtgagtg gtggaaaccg
    12481 gcatcccggt ataactcgcc aggccgattg acactgccgc cgtcatctgg gcagacatcc
    12541 cctgagcgta ggtcatgcct ttcttaccga ttttctcacc gatagtcgtt gccacacgac
    12601 gccagccaat catcgtaccg atacctaacg ccagcgcgac cgccatgatg atccacaccg
    12661 gcgcatactc gatggtgcta agcatgtcgg acttcagttt cttcaacagg cgctgatcgt
    12721 cagcactcac accaggcatc ttcaccactt tgtcgatagt gtcagaaacg cacagcataa
    12781 tgcggcgcat ctggctacgt tgatcaagcg acagcttgtc gtagctttcc acatcggtgg
    12841 tcaacatacc tttcaggcgg ttgagcgcgt taatggtatt cgacggatgg cagtggaact
    12901 ccgcaggttg cgttgcgcca gcttccggag ccggtactaa ctgatcagca ccggtagcct
    12961 gtttgagcag cgcaggatgc tgctcaaagt aagcttcgac gttgttgatg gcatcacggg
    13021 tacgggtgat ttcgtagcca gtggcattca tgttcaccac gaagcctgct ggcgcgacgc
    13081 caatcaatac caacataacc agaccaatgc ctttctgacc atcgttcgcg ccgtgcgaaa
    13141 acgccacgcc gatagcggaa aggatcagcg cgatacgcgt ccagaacggc ggctttttct
    13201 tgccgtcttt cttttcacgc tccgctgggg tcaggtggat acgggcgcgt ttcttggtgc
    13261 cgctccagta gcgacgcagc aagaaaatca gaccgccagc aaacaccagg ccgacaatag
    13321 gggaaacgat cagagaaccg aaaatactta atactttcgg gatattgagt gcatccacca
    13381 ctgacgtccc ggtcatcaac gcattggtta aaccaatccc gatgatcgcg ccaatcagcg
    13441 tatgagagct ggatgcaggt aaaccaaagt accaggtacc caggttccag ataatcgccg
    13501 ccagcaacat agagaacacc atggcaaggc catgagacga tcccatatta agcagcagat
    13561 ccgtcggcag catatgcaca atggcatagg caacactcag accacccagc aaaacaccca
    13621 aaaagttgaa caccgccgcc ataaccacgg cgagctgaga acgcatcgcg cgggtataga
    13681 taacggttgc cacggcgttg gctgtgtcat ggaaaccatt gatggcttcg tagaacagca
    13741 caaaagccag tgcaagcaat aataacagcc cggtatgcaa atccaggcca gcaaacaaat
    13801 gtagcatagg acgttacgcc attttgagga catgaacgcg gcgcattatc agtgactttc
    13861 gcggcgcggg caaagtgaaa tatagacttt ttttgatttg cctcctgtat ggatttcact
    13921 caaaaaataa ttatcttata taattcaggc aaatacttcc ttttagtaat attgatgctg
    13981 gtgcgaccac tgaggaatct ttacaattca cgcccgtttt ttctaagagg agcgcaacgt
    14041 ggaaaggttt gatgccatta ttataggcgc tggtgcggcg ggtatgttct gttctgcgct
    14101 ggcaggtcag gcaggacgcc gggttctgct gatcgataat ggtaaaatac cagggcgcaa
    14161 aatccttatg tctggcggtg ggcgctgcaa ctttaccaac ctttatgtcg aaccaggcgc
    14221 ttatctgagc cagaatccgc atttttgtaa gtctgcactc gcacgtttta cccagtggga
    14281 tttcattgat ctggtcaata aacacggtat cgcctggcac gagaaaacgt tagggcaact
    14341 cttctgcgat gactccgcgc agcagattgt cgacatgctg gtggatgagt gcgagaaggg
    14401 caatgtaacc ttcagattgc gtagcgaagt gctgagtgtg gcgaaggatg aaacaggctt
    14461 cacgcttgat ctgaacggca tgactgtcgg ttgcgaaaag ctggtcatcg cgaccggtgg
    14521 gctgtcaatg ccggggctgg gcgcatcgcc gtttggttat aagattgccg aacaatttgg
    14581 cctcaacgtg ctgccgaccc gcgcgggtct ggtgccattc actctgcata aaccgttgct
    14641 cgaagagtta caggtgctgg cgggcgtggc ggtgccttcc gtgattaccg ctgaaaacgg
    14701 caccgttttc cgtgagaact tactcttcac ccaccgcggc ttatctggac cggcggtgtt
    14761 gcagatttca agctactggc aaccgggtga atttgtcagt atcaatctgc taccggatgt
    14821 ggacctcgaa accttcctga atgagcagcg taacgcacat ccgaatcaaa gcctgaaaaa
    14881 cacactggcg gttcatctac cgaagcggtt ggttgaacgc ttacagcaac tcgggcaaat
    14941 cccggatgtt tcgcaaaaac agctcaacgt gtgtgaccaa caggcactga ttagcacatt
    15001 gaccgactgg cgcgtacaac ccaacggcac tgaaggctat cgcactgccg aagtgacgct
    15061 cggcggcgtg gacaccaacg aactttcttc acggacgatg gaagcgcgca aagtgcctgg
    15121 gctgtacttc atcggcgaag tgatggacgt taccggctgg ctggggggct ataacttcca
    15181 gtgggcgtgg tcgagtgcat gggcttgtgc gcaggatttg gcgtcaattg tgaaataacc
    15241 atgacgtttt cccgtcagcc gtttgtatat gtcgttatta agtcattttg gtacggttct
    15301 gcatccgaca ttctggtgtt aactcgctgt tttgttagcg aattaacgct attgccgttg
    15361 tggttatttt atagtgtatt tattctcaga gaatatatat tactgaaata aaaaacacaa
    15421 agaataacca aaggaaatta tttgaaacgt aaaaggtata tatttttaat ctgtaaaatt
    15481 taaaatcacc ccctctggat tgaatcaact tgctctattt aagacgattg tttttacatt
    15541 ttcaccacct gtatcaggtg gtgttttttt attggcggca atgcctttat cttattttta
    15601 ttacgttgta aatatggcta ttcctaaatg aaatttgatt tgctcagttt atttgtgatg
    15661 tcaggggagt gcttgccatg agagtcgtga tattattatt catcattttt ataccgagtg
    15721 tggcggatgc tttttcattc gatagttcaa taattaatct caaatgtcct aggcgtggaa
    15781 ctatcgaaat aatattacac agatacgaac acacccagga aagctgggga ggggagaatt
    15841 ttgaaaccgg gagtgggtat agtcatgagg gcgcgttatt tatttttgac tttgccaatc
    15901 aggataaaat gatttacaat cgcgcggaga attcgtttgc tttttggtat gaatcttccc
    15961 ggaaattagt taactgtaac ttaataacgc ttaaaagcac atatcctgtt aacctgcctt
    16021 atgttcatgg ctgatgtgat gtgtgtcggg aactaacatc ttcatctttg tatatgacat
    16081 tacgataatg ttaagctggt ctttgtcata aatttaccct tagagatact actcccttct
    16141 gtcaatatta ttcaagccga acgttatact ggcttttgtc ggtcacggat atcttttttt
    16201 cagtgagaat tcaaagtgag ggtatataag aagtcgtatg agtaagtatc tttgttgtgg
    16261 tgggttatta ttttatgggc aaaatattaa gcagcgaaat cttttttttg cccagacgtt
    16321 ttttgtagaa aaaagaattt attagtccgg ctgaaaagca agaactgcta taactaatta
    16381 agcgtaacgg aacggtacaa aacatgccct tacaacaatt ttcccgatta gcgctcactc
    16441 tggcgatcgg cctttcgatt gctgcgggtg gggccgcgct gacctggtat caggtcgctg
    16501 aaacagacct gagcagaaca gccgctgctg cccatgaggc ggtctcggtt actgaacgac
    16561 tgctggacga agcccagggc gcaatcgatc tggtttatcc cctgagtgtg caaccatgta
    16621 ccccacagct cagaaccgaa cttggccggt tgaccattag tcacgaacat atccgggtga
    16681 ttaacatttt taatcagaat cttttgtcct gctcgtcctg ggagggcgca gatcctgtgc
    16741 gggagttcat ctccgctggc gacaaccaga cgctgattct ggcgacagac gattatatat
    16801 cacctggtgt gccggtcatg attctacgca aaacggataa tgacaacgcc ataactgcga
    16861 gtatcgccac taaatgggtg gctgacaata tgacacttct gaatcttcgt aggccgctgt
    16921 cgctgcgcgt agggaatact gttcttacca gcgataacac gctggtgaaa gcgatacctg
    16981 gagggaatag acacgcagtt tattctggcc tgtatccctt ttccatagaa tatcctggcg
    17041 atctttccgt tcctctaacg ctttattttc atgatggtgc gttatcttta ctgctttctg
    17101 tgcttctggg ggccgctgtg gcaaccggcc tgtggaaaat aaaatttcgc cgtaagagcc
    17161 gctatgatga aatctacgag gcaatgagga aagatgagat tgtgccgtgg tatcagccaa
    17221 ttatggatgc ctcaacaggt gaaatagctg gcgttgaagt acttgctcgg tgggtaaaag
    17281 cagatgggga agtcatttat cccggcagtt ttatcactga agtagaacgt tcagatttag
    17341 ttattccatt gactcgtagc ctgatggcgc gggcatcccg ggatttactg cctctgacag
    17401 gcgacggaaa gcgttggcat attagtttca atattacgca gtcacacatt atggaaacgg
    17461 gatttattgc cgagtgcctg acgtttattg atgcttttac accgggcagt atcactctca
    17521 ctatagagct cacagagcga gaaccctttg ataacagcga tgagatgcga tgccagctta
    17581 agcgtattca tgatgcaggc attgcccttg cgctggacga ctttggcacc ggttacgcaa
    17641 atatggaata tcttagtgag atcccagtcg atatcatcaa aattgatcgc gtctttgtga
    17701 acagaatagg tcagggcgaa tctgcagaac acctgctgac cagccttatc gagatggcca
    17761 atacgctgaa gaaagagatt atcgccgaag gtgtggaaac cgaggcgcag gccgcgtggc
    17821 tatatgagcg tggtgttggc aagttacaag gattcctata ttcaccggca ttaccgcccg
    17881 ataagttggc actgtttaaa ccgaaaatgt cgtgagttca acggtacgcg ctatcggaat
    17941 cattgaaccg gtgagggccg ccccctttat tactcagggg gggctttcaa cgttctgaat
    18001 taagatgttt tatgactcac tgagcaggat gtgagttcca acgtaatctc cgctattttt
    18061 actgtttttc atttgctgcc tcatctatcc cttcgccttg cctctgagtt gcgttaaagg
    18121 ctgatgtact gcctttaacc cgttcaatac atagcctttt tgtcaatcat catcccgaag
    18181 agcgttgcac tgtgccttcc atagctgtgg ttgtcctctc aatctgtcag cccatttatg
    18241 gaaaatctta acgaagcaac aatgagatat gtaaaaaaag ggccgattta atcattcatt
    18301 aagacaggtc attttttctt actgtgaggg cggtaagctt ttctgtcggt aacaggttgg
    18361 gagatgctgg tattatgaga tttttctacc gtcctgcggc ggaatatgtg aaaaatagaa
    18421 atgattatta ttattgcatg gttattttta gtttgtgatg gtggagaata atcctattcc
    18481 cgtgaccttg ttttttacat tttatttaac tgcatggtat atgcattt
//
LOCUS       DADLSB010000076        18418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.076, whole genome shotgun sequence.
ACCESSION   DADLSB010000076 DADLSB010000000
VERSION     DADLSB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.076"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..810)
                     /gene="ugpC"
                     /locus_tag="JN331_002437"
     CDS             complement(<1..810)
                     /gene="ugpC"
                     /locus_tag="JN331_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY7260562.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLK"
     gene            complement(824..1483)
                     /gene="pgmB"
                     /locus_tag="JN331_002438"
     CDS             complement(824..1483)
                     /gene="pgmB"
                     /locus_tag="JN331_002438"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY7260563.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1480..3747)
                     /gene="ycjT"
                     /locus_tag="JN331_002439"
     CDS             complement(1480..3747)
                     /gene="ycjT"
                     /locus_tag="JN331_002439"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAY7260564.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGYVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(3744..4799)
                     /locus_tag="JN331_002440"
     CDS             complement(3744..4799)
                     /locus_tag="JN331_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7260565.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCGSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAWEMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQLIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4809..5597)
                     /locus_tag="JN331_002441"
     CDS             complement(4809..5597)
                     /locus_tag="JN331_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY7260566.1"
                     /translation="MKIGTQNQAFFPENIRERFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5615..6667)
                     /locus_tag="JN331_002442"
     CDS             complement(5615..6667)
                     /locus_tag="JN331_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAY7260567.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6698..7540)
                     /locus_tag="JN331_002443"
     CDS             complement(6698..7540)
                     /locus_tag="JN331_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY7260568.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7527..8408)
                     /locus_tag="JN331_002444"
     CDS             complement(7527..8408)
                     /locus_tag="JN331_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY7260569.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8429..9712)
                     /locus_tag="JN331_002445"
     CDS             complement(8429..9712)
                     /locus_tag="JN331_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7260570.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNRQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLASLTITAGQKTEAAEKFVTFMEQADNIADWVMMSPG
                     AALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDVTGS
                     GVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9726..11403)
                     /locus_tag="JN331_002446"
                     /pseudo
     CDS             complement(9726..11403)
                     /locus_tag="JN331_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11615..11962)
                     /gene="pspE"
                     /locus_tag="JN331_002447"
     CDS             complement(11615..11962)
                     /gene="pspE"
                     /locus_tag="JN331_002447"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY7260571.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIVTAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(12005..12226)
                     /gene="pspD"
                     /locus_tag="JN331_002448"
     CDS             complement(12005..12226)
                     /gene="pspD"
                     /locus_tag="JN331_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY7260572.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12235..12594)
                     /gene="pspC"
                     /locus_tag="JN331_002449"
     CDS             complement(12235..12594)
                     /gene="pspC"
                     /locus_tag="JN331_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY7260573.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12594..12818)
                     /gene="pspB"
                     /locus_tag="JN331_002450"
     CDS             complement(12594..12818)
                     /gene="pspB"
                     /locus_tag="JN331_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY7260574.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12872..13540)
                     /gene="pspA"
                     /locus_tag="JN331_002451"
     CDS             complement(12872..13540)
                     /gene="pspA"
                     /locus_tag="JN331_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY7260575.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDKAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13707..14684
                     /gene="pspF"
                     /locus_tag="JN331_002452"
     CDS             13707..14684
                     /gene="pspF"
                     /locus_tag="JN331_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY7260576.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14804..16069)
                     /gene="puuE"
                     /locus_tag="JN331_002453"
     CDS             complement(14804..16069)
                     /gene="puuE"
                     /locus_tag="JN331_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY7260577.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(16107..17387)
                     /locus_tag="JN331_002454"
     CDS             complement(16107..17387)
                     /locus_tag="JN331_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7260578.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(17389..>18418)
                     /locus_tag="JN331_002455"
     CDS             complement(17389..>18418)
                     /locus_tag="JN331_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7260579.1"
                     /translation="VREPVGVIAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSP
                     LSAIRLAGLAKEAGLPDGVLNVVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKD
                     AGDSNMKRVWLEAGGKSANIVFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEES
                     IADEFLALLKQQAQNWQPGHPLDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGR
                     NAGLAAAIGPTIFVDVDPNASLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAV
                     WTRDLSRAHRMSRRLKAGSVFVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELK
                     TIWISLEA"
BASE COUNT         4294 a         5057 c         4648 g         4419 t
ORIGIN      
        1 tttcagaacc gctaatttct cttcgggaat ggttaattta agcgtttccg taacgaattt
       61 atcgccatcg atcgtgccgc gaataaaatt catcgctggt gatccaataa atccggcaac
      121 aaacatattc gcgggttggt tataaacggt tttcggcgca cctacttgct gaacaatccc
      181 gtctttcata atcacaatcc gcgtcgccat ggtcatcgct tcggtctgat cgtgggtcac
      241 gtagatcatg gtggtgttca gtttctgatg cagcttgctg atctctgcgc gcatttgtac
      301 gcgcagcttg gcatcaaggt tagagagcgg ttcatccatt aaaaacacgc ccgcttcgcg
      361 cacaatcgcc cgcccgagcg ccacacgctg gcgttgcccg ccggaaagcg cgcccggctt
      421 gcgtttcagg tactcacgca ggccgagaat ttgcgccgcc cagttcaccc gctcatcaat
      481 cacctctttg gcgatttttt gcatcttcag gccaaacgcc atgttgtcgt aaaccgtcat
      541 atgcggatac aacgcgtagt tctggaacac catcgcgata ttgcgtgctt tggctggaac
      601 gtcattcatt cgtttgccgt cgatcaacag atcgccgccg ctgatctcct caagcccggc
      661 aatcatgcgc agggtggtcg atttaccgca gcccgacggg ccgacaaata cgatgaactc
      721 tttatcggca atttccaggt tgaagtcctt caccacatgc acctggttat cgtagatttt
      781 ttgaatatgt tgtaacgaaa gctgagccat gttgattcct ttgctatacg ttttgccaga
      841 aggccgataa ccgcgaccag gtcagggagt ccgttgaggg caacagtaat tgcgccccgg
      901 ttaagcccgc accgatcccc accgagcgca taccgctggc gttaatggcg tcaatgcccg
      961 cctgcgcatc ttcaatgccg atacatgcct gcggcggcac gcccagcccg gcacaggcag
     1021 cgagaaagat ttccggatcc ggtttcgagt ttttaagttg ggaagcatcc gcgcagaagg
     1081 tgaaaaactc gcgcagctcc agcgccgcta aaatcgtcgg cgcattcagg gagacggaag
     1141 ccagccctac cggaatctgc tgtgcacgga gctctgccag caaattgcga ataccgggca
     1201 gcacagcatt aaccgtcagc tcgcgcagtg agtggacgta aagcagattt ttgcgatacg
     1261 ccagttgcgc ccgctcctgc gggttaaagt cgccctcttt gcccccgtgt tgcagaatgc
     1321 gccgcagaga ctcatcgcgg ctgatccctt ttagagattc gttaaactgc gcatcaatac
     1381 tgatgccaat ttcagcggca atctgctgcc acgcctggaa atgcagatgc gcggtatcgg
     1441 tgattacacc atccagatcg aaaattaccc cttgcagttt cattcatcct cctgatgcgt
     1501 ggtagcggtc ccattgaagg gcaaaataaa atcacccaaa cagaaaacag attcttcagc
     1561 cacggatata agctgaccgt tcaaacgcag tgaaacgggc gcagaagttc gaatcgcaat
     1621 acgctgcgcg tcgagagtga cctgtaattc gcagccctgc cagaacagag ggaaagacaa
     1681 ctgttgccac tgctcaggta acgccggatt tagatgcaat tcaccgtcac gcacgctcac
     1741 cccggcaaaa ccctgaatcg cccccagcca gatagcgcca gttgcggcag catggatacc
     1801 atcatcacaa ctatgcggat cagcaccaag atcgatttca gtcccctcgc gccagaactg
     1861 ataactttgg gtcagcaggc cacagcgtgc agcaacaatg ccgtggattg ctttacttaa
     1921 tgacgagtcg tgaatagtgc gcggttcata aaattgcaga ttggcaagac acgatgccga
     1981 tgagaactgc tctggcagca tgtaattgag catcaccaca tcagcttgtt tgaggatctg
     2041 catctcgttc acttctgcgc gtgaataatc cagcagaatg gtttgcttcc ccgccgccgc
     2101 tttgtatttc gccagattaa tcgccggctt agtcataaac gaatcatcct gcggcaaaac
     2161 accgtcgggc tgaatttctg gcagccgcag ttctttaagg aacatttcgg cacgatgaat
     2221 aaacgcgtcg tcactacagc caaactggcg ggcaatactc agcgcctgct ggacgttgta
     2281 atacaccata tagctggtga aggcgttatt attgacatgt tcggtatatt cgtctggccc
     2341 aataacatca tgaatttcca gacggtcgtt aaccctcacc gcgcggctaa tccagaactt
     2401 tgcagtttcc agaagtagcg ccatgccttc atgcgcaatg aaactttcat cccccgtggt
     2461 ctgccagtat tgaataaccg cccaggcgat atcggccacc agatgatgtt ccgcctgcgc
     2521 cgaggccact ttttgccgca gcccggtgcg aatgttaatg gcggcaaatt ccggcgtctc
     2581 ttcttcgccg ctgcgcgcgc tttcccacgg aaatagcgcg ccctgccagc cgttgcgtcg
     2641 cgctttctcc tgcgcgcctg gcaagttgtg ccagcgataa cgcagtaaac ttcgggcaac
     2701 cgtcggatcg ctaaacagat ggaacggcag caaaaatact tctgtatccc agaaaacgta
     2761 gcctttgtag ccttcgccgg ttaagccttt tgccgcaata ctgctgcgct cgtcgtgagc
     2821 cggcgtcatg atacgcagat gataaagcgc ataatctaac gcttgctgat cgtgcgcatc
     2881 gccgccattt accgtgatac gacgtttctg ccaccattga cgccagtttt ctgttgatgc
     2941 tgcaagatgt tggtcgtaac tctgttgcgc gcacatttca agctggcgaa gcgacacgct
     3001 gccccactcg tctaaagcag cttgcctgtc atcccgccag tcgatccaca ccagtttttg
     3061 caacgtcact gtctcgcctg catgaagctg cgcactggta tgttgcagta aacggcgctc
     3121 tttggcggta tagcattgct gcacatcacc gctcacctgg cagcaacaac tgatggccac
     3181 atcactgcgc ccatcctggg tggtgtagat cccctgcatc aaatgctgac caaacacccg
     3241 cacctgggtt tcgtcgagat gctggcgacc gtggttggtt tgcgtggcgt caatgcctgt
     3301 tgaaatcagc actgacgcgt cggcgtccag tggcgtaata gtgatttcca gcgcaatgag
     3361 cggcagttgg tctgccgaaa caaagcgacg gctggcgatg gtgtaacctg cgccgttgct
     3421 ggtacgccag acaacgctac ggcgtaattc tccactggca aagtcaagtt cacgctgcca
     3481 ggcttcgcgg gataacgaga aaacctcacc atttatggca atctccatcc ccaagatatc
     3541 aggcagattc accagttcgt tgatttcacc ttttcccgcc cgatgataca gccccgccag
     3601 atacatccct cgcgtctggc gcgtgtaatc ttcttcatgg ctggcgcgaa gcccaagata
     3661 gccgttcccc tgcgccatca gcgatgcata cttgttcagg gtatgctggc tgaaatgggg
     3721 ttctgataac gttactggcc tggtcataat tccacacgcg tccctgtttc tgcggattga
     3781 tataacgccg ccaccagttg ctggatgatg tacccctgct cggcgtcggc tatcatcacg
     3841 ggcttgccct gtacgtgatt gataaaggct tccatgctgc gcaaatggcg gttgtcgtct
     3901 gccatttccc gttgcatcag cgtcattaat tcaccgttgt tatcggtgta gatatgtgct
     3961 ggaaacagcg tcgcaccggc tttatcacca caaaagctga cgttcataat caactgttcg
     4021 cggatgttga gtgcaaatga cgtttccagc cacagaatgc cgccgttatg aaattcaatg
     4081 gtgccaaaca gcgaatcttc gacgctgtaa gttgccggat cccactcgcc aaattgacca
     4141 cagctctttt gcgtgccgat cttttgaaaa ctatgcgcat tcacgctttt caccgccgga
     4201 aagcccagca catacatcgc agcatccagc atatgaatgc cgatgtcgat cagcgggcca
     4261 ccaccctgca gttctttatt ggtaaagaca ccccagccgg gaacgccgca gcgacgcagg
     4321 gcgcgagcgg tggtaacgta aatctctccc aaaacgccgt tggtcacctg ttcacgcagc
     4381 tgttgcgtat cgagcgcaaa acgatggtga aagtcgtagg ccagcacctt gcccagtttg
     4441 cgcgcggtat cgcacatttc ccacgcctgt tctggcgtca tggcgggcgg tttttcgcac
     4501 atcacatggc agcctgcttc cagtgccatc agggtatgtt cgtaatgaaa acggttaggt
     4561 gagcagacgc taaccacatc aggtttcacc gccagcagca tggcctgcgg atcgtcccac
     4621 acggatgcat tcccgtattt ttctgccagc gcctgcgcct gggaaaggcg gctgccacag
     4681 acagccacca gttccagatc gttgcgggtg cagtagtacg aagcatgaac cttatccgcc
     4741 acctggcccg cgcctattat cgcgacccgc agcggagagc ttgtcattgc acttttcaca
     4801 aagacctctt agcaggtacg caaccaggcc aacgaatcac ggtacgcctg ggcaggatct
     4861 tctgcccgga tacgcccttc atacaccaca taaccctgat agttatcggc gcgcagctgt
     4921 tcaaacagcg cgtggaaatc gagggtgcca ctgcccggct ggtaacgatg gttatcggca
     4981 atatgcacat gaccgagcaa gtcacggttg tcgtgcagcg cctgcgccag gttatcttct
     5041 tcgatgttca tgtgatagaa atcgccgata atctgcacat gtttaagatt gttttcgacg
     5101 atgtaacgac gagcatcggc gagggtgttg atcatatgat cctgatagcg gtttaacggt
     5161 tcgagataca ccacggttcc ggtacgcgcg gcgacctgtt ccagtacgcg cagggaatca
     5221 ctcaccattt tgcggtcgcc gtccaggcta cgcggcgagg tcatcggcgg taagcggaag
     5281 gtaaacatgc cccacgctgc cggaacgacg atacctttac cccccacttc tgccagcgcc
     5341 tcgagaatgc gttcgatctg ctttaagcca ttaagacgac gctcttcgat aaagtcacca
     5401 atccagccgt catagccgcc gcaggcggtg gtcactggta aaccggtttc tttgatcgcc
     5461 gctttgactt cttcgatgtt gttgaccagc agtttgccgt caatctcaaa accatcgaag
     5521 cccatctctt tgatataacg aaatctctcg cggatatttt ccggaaagaa tgcctgattc
     5581 tgtgtgccga ttttcatttt ttcattcctt tgaattaaaa cgtaacgccc attttgatgc
     5641 tctgttccgg atgctgatcg acatactgca tgtagctttc cgggctgttg gcaaaggtca
     5701 ctaccgggtc gattaaatct tcgcaattga gataaccgtt catcagcagt tcccagcagg
     5761 tttcttcaat acgcttacgg ctccagcgcg gataatccgg gttcggttcg ctgcacgcgc
     5821 gggagaaaac aattttggcg ttattgaaat gcgcttcgcg tccgaggtta aaaccttcag
     5881 caaatggttt ggcaaacgcg acataggaga tggtgccgcc gtaggccagg ccgcgcagcg
     5941 ccgattgcag cgcgtcggcg taaccgctgg tttcgataat cacatcggcc ccctgcttgc
     6001 cggtcagcgt tttgatctct ttgcctacgt cagtgccaat ggggttaagg cagaaatcag
     6061 caccgtggcg acgggcaata tcacagcgat gggcgatagg atcgacgcca attaccaccg
     6121 atgcgccagc gcgtttcgcc agttggatgg caatttgacc gatcgcgcca agccctacca
     6181 ccacgacaaa atcccctacg cgtacgttgg catcgcgcac gccgctcatg gcaaactgcg
     6241 ccgggtcgta acagacggcg tttttccagg agctgccttg cggcattttg cgcagcttgt
     6301 agttattcac tgcgttaatg atgaccgtct cggagagcgg gccgtagccg catacgctgt
     6361 cgcccaccgc gtagtctgta acgtcgctgc cgcactcgat aatgtcgcca accaccatgt
     6421 tgccaagctg gaatttgcca aactcaatgc cgcgcggcgc atctgccgga cgcggggtga
     6481 acatctgcca ctcgccgtta aagtcttcat cgataaacgg gctggcggcg cggaagtcga
     6541 ccacttccgt tccgtgtttc ggtgcgccga aacgcacgcg gatcttcact tcattggcta
     6601 aaatcgcccg atcttcatac tcaaccagcg cagcaacacg cggtgctgtg gctactaact
     6661 ttttcatgta aaactccttg taaagaaact ggccgcttta gcccttcacg ccaccggcgg
     6721 tcaaaccact cttaataaaa cgttccgaaa gcgcatacat aatcaccacc ggcaatgcgg
     6781 tcaccagcga ggccgccatc atccgtcccc agatgtaatc aggcgtactg aacagcgcgt
     6841 tcaggcccac tggtaaggtg aaattgctgg cgctggagag gaaaatcgac gcaaacagat
     6901 agtcgttcca cgccaccata aagcagtaga cgaacaccga tatcagcccg gacatcgcca
     6961 ggggcacggt gatacggaag atgatttgca gacggttgag gccatccatc atcgccgctt
     7021 cttcgatctc atcagggatg gtgtcgaagt aacttttcag cataaacacg gcggtgggca
     7081 gggtttgcgt caccatggta atgatcagcg ccatctcggt gtcgtaaata ccaagcgcgg
     7141 tgatgatttt gaacagaggc accaccagca aaatgccaga gaacatatac accgtgtaaa
     7201 agctggcgtt gatggtcatc cggcctttaa agcgcaggcg ggaaagcgcg taagctccga
     7261 gaatgccgag aaataccgcc accacggagg aaaccaccga caccaccatg ctgttacgga
     7321 agtagtcgac aaacgggaaa atcatcgggt taaaaatgtc gacgtaatgc tccagcgtcc
     7381 actgttgcgg cagcagcgta ggatgcagcg atatcgcctc tttcgcgctt ttgaacgagg
     7441 tcatcagcat cacaaaaaac ggaaacagcg tgatgatgag aaacagcgcc agcccgcagt
     7501 aaaaaccgat acgactgagc gtgcgtttat ttgttgccat tgaggttcac ccgtttcctg
     7561 gtcagcagaa tgacagcgaa aataatgatg aagagcacca ccgagatcgc cgccgctttc
     7621 cctaagtcat taaaggcaaa ggcggttttg tagagataga caccaagaat atcgaccttg
     7681 gttgtcagca gataaacatc agcgaacatg tagaacatcc agatggtgcg cagggtcacc
     7741 accgtcgcca ggaccggcat aattgcgggc agcgtgacga tgcgaaaccg ctgccaggca
     7801 ttagcgccat ccatctctgc cgcttcgtac agcgatttgt cgatggtctg caaaatcgcc
     7861 agaaacgaga taaaggcata cgggaagtag cgccagatgg cgaacagcac caccagcaca
     7921 aagctactgc ccggattatc aaaccacagc ggtgcctgct catagagatg cagaagatcg
     7981 acgccgaggt agttcacaat gccgtagccg ttgttgaaca tgtatttcca ggcaaacacc
     8041 agcgaaatgg acggcgttac gtaggagagg atcaccagcg aacgcgcggt tttgcgcagg
     8101 cggaactcgc ggttgaaaaa catcgccacg gccagcccga gcacggtgct acccgccacc
     8161 accagcgccg tataccagac cgtcatccac agcgagtgcc agaagccggg atcggagaga
     8221 atgcgcacat agttactcac cccaacaaac gttgactcga tgttgggatt gagcggcaga
     8281 cgtaaaaaac tgatttcgat attcgacacc atcggccacg ccaccagacc gcccagcagt
     8341 aataagctgg gcgcgagaag cagcagcgca aacggcatat cggaacgacc tgaaaaaagc
     8401 ctgttcattg cggattcctg tcctgggttt agtgctgttc gatcagttca tccagctttt
     8461 tctggctcgc ttgcagcgtg ccagggagat cggctttacc cacggtgacg ttatgcacca
     8521 tggagctcac cacaccagaa cccgtcacat cgcccatgcg ggtaaagttt ttatccccta
     8581 ctgcgccaaa gacctgaata tttggcagtt caccgattag ctgattcggt agttcaccca
     8641 gcgccttaat aacgtcgttg tctttccagg tggcggtagt gacaaccgct ttattcaccg
     8701 gcagcgcagc acctggcgac atcatcaccc agtcggcaat gttgtccgcc tgctccataa
     8761 aggtgacaaa tttttctgct gcttcggtct tttgtccggc ggtaatggtc agcgaggcca
     8821 acatgccgta gaccgcagag tttttctcgg ttggcaccac gaaaccgacg tttttcgggt
     8881 cgccttcttt aatcacagcc ggaaggatat aggtggagta aatcgccatc ggcgcggtgc
     8941 cgttcataaa ggcgtctttc acttccatga tgtcgttaga acccggcatg gtgttggcag
     9001 caaggtcgcg gtaataggtc agcgcctgca tcatctctgg tgtatcaagg gtgattttgc
     9061 cttcggcgtt aaagacgttg gcctggttgg ataacgcaaa ctgggaaaag gattgttccg
     9121 tcagcacgct ttctgccgta ggcagcgcaa taccgtattt tttattcgcc gggtcattca
     9181 gtttctgtgc aacgtccagc agctgttgcc tgtttttcgg ctcctccagc cccgcttttg
     9241 ccagcacatc tttgcgatac cagatgccgc caatccaggc gctgacagga acaccggtcc
     9301 atgcgctacc atcttcggta cgcacaatac gcagtacgcc atcgtaaaac gcgccttcac
     9361 caacgttgct gatgaccgtg gcaaccgctt tgcgatcgat aagctgctct ttatccataa
     9421 ctttggcgta gtcatggctg gtttcgatca cttccggcag cgagccgcta cgtgaaaggg
     9481 tgatgacttt ggtgttataa gcgtcttctt ccacgggcac ttgtttaacg gtgatacctg
     9541 ggttttcttt ttcaaaacgg gcaatcaatt tgctgataac tgcctgacgc tcctgctcca
     9601 ccgaagaatg cataaattcg atggatacat gggttttatt ttcttcttta cagcctgaaa
     9661 tcagggcgca ggaaaccagt gctgataaca gcacgatttt tgatttaatc atgagatgtt
     9721 cctttttatt taatccacat aacctgccag gggcgcagcg ttatttcatt acccaatata
     9781 tcaacttcac taatcagatc ccgaccatgc agatcagtaa tatttacatg ctgaatattt
     9841 ttactgacat taaacaatcc agtcagacaa ttaccatcag cgttacttct ttgaatacgc
     9901 attacggatg aattaaccgt atcgatggta aaattattat ccggatgaaa ctcgttatgg
     9961 ctgcgacgaa gtgtaattaa acgcgacaac tcatgatata ccgcatgcct taatgtagct
    10021 tcatcgttca gttctcgtgt tatctcttca ctgtgatatt ttttacggtt aatcgcacgg
    10081 ttatatccga gtttttcgac acctgcgtaa tcattacgcg agccaagaat actttgaata
    10141 tatatcgcgg gtacaccggg gaaacttaac aaaatcgcat gggcaaggat aaacctggcg
    10201 caacgttctt catcgctact ctcgcggcgg cttaacgcat ccatataggt gacattcatc
    10261 tcatagggac tgcgcgtgcc gtcgggatta tttttccagt ttactaatgc accttcctgc
    10321 tgtaatgcct cgaccagcgc taatatttcg ctttctggaa gcaagccacg cagtgggttc
    10381 aacccgatgc catcgtgaga ggcgaggaag ttaaaccagg tcgttttact ggagggcaag
    10441 ctcagacttt gcgcccactg gcacagcgcc tcgacgttct gtttttgcac cgcatgtaac
    10501 accagcggcg gcagcgagaa ctggtacacc atatgcgctt cgtcatcacc gttgccaaag
    10561 taagcaatgt tgtctttgtg cggaacattg gtttcggtaa tgatcactgt acctggtgct
    10621 acgtcatcaa taatcgaccg taacagcttg ataatcagat gtgttttttc cagatggatg
    10681 cagcttgttc ccggctcttt ccacataaag ccaacggcat ccaggcggac atactcagca
    10741 cctttctcaa ggtaacagag taaaacatcc accatcgcca gcaacacttc agggctacgg
    10801 taattcaggt caatttgagt cactaaaggt ggtccataaa tggcgcgtgg aattatcatc
    10861 catttgaaac ggcgttaata gcggtaacgc acgcggacgg gtgacggcac tgagatcggt
    10921 tttcggatca accgcaataa agaaatcttc aaaacccgga agctgttgta aatagttttt
    10981 aaaccattca ctttttgcag acatatggtt gcagacaaaa tcaaacatta aatgactgca
    11041 ttcaccgagt tgctgaatat cctgccactc ccccgcttca ctggcgacct gatgataatc
    11101 aattaccgaa aatccatcat cagatgacca tgggtaaaaa ggtaataaat gaacatgtga
    11161 aaaaatgctt tgcagccatt gatggtagaa ctgattaaat atgggtagtg gttttaaatc
    11221 attactgtga aattgatcgg cataggtaat taacacgaca tcactttcat cccagtgttt
    11281 tttacgtcgc tgtgtaatta atcgtttcgc actctcaaga cgcgttacaa gtttctgtaa
    11341 atgagttgcg gtaaatgttc caccgtagat ttcgtccagg taatccgtaa ttttctgttt
    11401 catgttgcct tttcgtggta gcggtcccat gaagcgagag actactccgc agaaaataaa
    11461 ctgtcaattg agcacaaggg ttttagtcac gcaggaaatc attttttacc agggaaaaag
    11521 cgtatttgtg agctgacgca aataatctat agggatgtaa ataaggtgtg acagaaaaaa
    11581 aacggcgcat aagcgccgct catggtgaat tcttttaacc tttgaccttc ggcattgcga
    11641 tgtctttcag gccaccggcg ttctcaacgt gggtatatcc catctcgcta aggatctctt
    11701 ttgcctgccc ggactggcgt ccggcattgc aatacacttt cacggtgtcg tttttatccg
    11761 gaacggcggt gacaatgcgc tctttcactt ccttcagggg aatattgatg gccccctgaa
    11821 cgtgctcttg ctgatactgc tctggaacac gaacatcgat ccagtgttca gcggcgaaaa
    11881 cgggcagtga aaacaccagt gccagagcaa gtaagccttt ttttaaacat gtaagcgtcc
    11941 ttctatggat gaaaattgcc atccattata cgcctgagat ttaacaattt ttgcgaaact
    12001 cccctcacct tttataacgc tgtgccagtt tattagcagc ccgacttaac agcggttcca
    12061 gcgccacagc cagcaacatt ttcagcggtc ggcgagcaac tgattttatc gcccagcccg
    12121 ccacacccgc cgggccatag cgcagtgcgg taagaagtac cagcttgcct gctaatttga
    12181 aaccaggctt caccttttgc ccggcctgtt gccagcgagt attcataact ttcctcacag
    12241 ttgacggaaa cggctacgta acgtgaaagt atcggaagtg acataacgtt ccatctcgcg
    12301 taaacgcgtt tcacttgccg ccagttcacg gtcgacttca tccagcaatt cgctgctgga
    12361 aggtagctgc tcaccaaagg ccatgttgtc cggcattgga tcaagcgcaa atgacaaaat
    12421 gatgtaagca accagggtaa acagcgccag accgaagaaa atcgacagca ccaccaggat
    12481 acgcaccagt tttaccggta catcaaaata gttggcaatc ccggcgcaga cgccgcggac
    12541 catgccctgc tgtggaatac gccataattt tttattgaga ttaatgcccg ccattagcga
    12601 tccctccagt tcggatgttc tgcatcaaga atagattcca gcgcctgaat acgttcgcgc
    12661 atccgttttg cttcatcagc cagttgcgct aatcgctgct gctcactttg cgacaattca
    12721 ctgcgaccag aacgattgct gtaatgcagc cataaccaga tcggtaaaac aaacagcaca
    12781 aaaatggtta acggaatagc cagaaatagc gcgctcataa gtactcctta gacgatgagc
    12841 ggcggcgcgt tcggacgccg cctggatgtt attattgatt gtcttgcttc attttggctt
    12901 ttaattgtgc cagttgttcg ctgattgcat catcggcttt cagttcggca aactgatcgt
    12961 ccagcgattt ttgtttaccg aagctgtggc tttctgcttc cgcttccatc tggtcaatac
    13021 gacgttcgaa agattcgaaa cgagccattg ctttatccag tttgccactg tccagctgac
    13081 gacgcacatc gcgcgacgag tttgccgcct gatgacgtaa catcaatgcc tgctggcgag
    13141 cgcgtgtttc gctcaatttg ttttccagct cgccaatctc tttcttcatg cgtgccagcg
    13201 tatcgtccac cagcgtcact tcatgttcca gggacttaat cagatcggtc agtttctgtt
    13261 tttcaattaa cgctgcacgt gccagatcct ctctctcttt cagcagcgcc agttcggctt
    13321 tttcctgcca ttcaacttca cgcgccgacg cttgttcaat acggcgagtc agctgtttct
    13381 tttctgccaa cgcacgcgcc gaagtagaac gtacttcaac cagtgtatct tccatctcct
    13441 ggatcatcag acgcaccagt ttctgtggat cttccgcttt ctctaacaga gcgttgatgt
    13501 tggcattcac gatgtcggca aagcgagaaa aaatacccat aatgttgtcc tcttgatttc
    13561 tgcgttcagg attgtcctgc tgaactgtta atacaatttg cgtgccaatt ttttatcttt
    13621 ttgatttata aagatctgat tgaagaatca acagcaacat gccaggatga attagcgaat
    13681 tacactaaca agtggcgaat ttcatcatgg ccgaatacaa agataattta cttggtgagg
    13741 cgaacagctt tctcgaagtg ctggaacagg tttcgcatct cgcaccgctg gacaaaccgg
    13801 tgctcatcat cggcgaacgc ggcaccggta aagagttgat tgccagccgc ctgcattatc
    13861 tctcctcccg ttggcaaggg ccgtttattt cccttaactg cgcggcgtta aatgaaaatc
    13921 tgctggattc cgaactgttt ggtcacgaag cgggggcgtt taccggtgcg caaaaacgtc
    13981 atcctgggag atttgaacgt gccgacggcg gtacgctatt tcttgatgaa ctcgctacgg
    14041 caccgatgat ggtgcaggaa aaattattgc gtgtgattga gtacggtgaa ctggagcgcg
    14101 ttggcggtag ccagccgttg caggtgaatg tgcggttggt atgcgcgacg aatgccgatc
    14161 tcccggcgat ggtcaatgaa ggtacttttc gcgctgacct gctcgaccgg ctggctttcg
    14221 atgtagtgca actgccgccc ctgcgcgagc gcgaaagcga cattatgctg atggcagaac
    14281 actttgccat tcagatgtgt cgggaaatca agctgcctct gttcccgggg tttacggagc
    14341 gcgccagaga aacattgctg aattatcgtt ggccgggaaa tattcgtgaa ttgaaaaacg
    14401 tggtggaacg ttcagtgtat cgccacggca ccagcgatta tccgcttgat gacatcatta
    14461 ttgatccctt taaacggcgt ccacctgaag aagccatcgc cgtttcagaa aatacctcgc
    14521 ttccgacgct gccgctggat ttacgcgagt ttcagatgca gcaggaaaaa gagttgctgc
    14581 aactcagttt gcaacagggg aaatataacc agaagcgcgc ggcagaatta ctgggtttaa
    14641 cttatcatca gttccgcgcg ttgttgaaaa agcaccagat ttagcgaagt tgagcaatac
    14701 aacttgccgg atgcggcgtg aatgctttat tcggcataca taatcaatac gtcgtttgca
    14761 ggcctgataa gcgcagcgca tcagacatta ttgcgttggg ctattaatcg ctcagcgcat
    14821 cctgcaaaat tttcattgcc gcatcgaatt gcgcatccgg gatggtcagc ggatacagga
    14881 agcgaatcac gttgccgtat gcgccacagg tcagcaggag cagcccctgc gccagcgcgc
    14941 gttgctggat tttctgtgca atcgccgctg acggctcgcc cgtttgcgga tcgttaaact
    15001 ctgccgcaat catcgacccc aggccgcgta ccgcagcaat ggccggaacg ctttctttgg
    15061 catcaatcaa cgtgtttttg agacgctggc ccagttgatt cgcgcgttcg cagagtgatt
    15121 ctttgtcgat aatattgagc accgcgtgcg cggcagccac cgccagcggg ttcccggcgt
    15181 aggtgccacc caacccgccc ggcgcgggcg cgtccataat attcgcgtta ccgaccacgc
    15241 ccgaaagcgg catcccgccc gcgaggcttt tcgccatcgt cattaagtcc ggcttatcgg
    15301 cgtaatgatc catggcaaac agtttaccgg tacgcgcaaa gccgctttgc acttcatcgg
    15361 caatcatcac aataccgtgc tcgtcgcaca ggcggcgaat ggcggcaacc agctcttttg
    15421 gcgcaacgtt gaaaccgccc tcgccctgca ccggttcgaa aataatggcc gcgacctgct
    15481 tcgcttcgat gtctgattta aacaagcgtt cgatggcgtc gagggagtcc tgtgttgaaa
    15541 tgccgtgtaa atctgacgga taaggtacgt gatacaccga accagggaac gggccgaagc
    15601 cgattttgta cggcgcaact tttccggtca gcgccatggt catatatgta cgaccgtgga
    15661 agccaccgct aaacgcaatc acgccaggtc gtccggtatg ggcgcgagca attttcaccg
    15721 cgttttccac cgcttccgcg ccggtagtga agaatgcggt tttagcctgc ccgctcactg
    15781 gggcaagggc gttaattttc tccgccagcg tgacgtagct ttcgtacggc acaatctgat
    15841 acgccgtgtg ggtaaactgt tgcaattgct gctccaccgc cgcgaccaga tcagggtggc
    15901 gatgcccggt attcagcacc gcaatgcctg cggcgaaatc gatgtattcg ttgccctcta
    15961 cgtccttcag cgtagcgttt tctgccgact gggcgaagaa gttacacatc acgccaaccc
    16021 cgcgtggggt agcagaaaga cgacgctgat ggaattcatt gttgctcatg tttcacacct
    16081 tttgactctt cactgttaat cggaaattaa acgcccagac gatcgcgcag gctgtaataa
    16141 gccgcgccca tcgcggtaaa tggcacgcgc agcgtgcgcc cgccggggaa cgggtaatgc
    16201 ggcagattgg cgaaggcatc gaaacgctcg gcgtcaccgc gcagcagttc agcaatcaaa
    16261 cgcccggcaa gatgggtaca ggtgacgccg tggccgctgt aaccctgcat gtaatagatg
    16321 ttggtatcaa ggcggccgaa ctgcggcatg cgcgacaagg tcagcaggaa gttgcccgtc
    16381 cagcggtaat caattttcac gcccttcaac tgagggaagg tttttagtag tttcggtacc
    16441 acaagacgct caacgtcatc cgggttgcgt gcaccataca ctacaccccc gccgtacagc
    16501 aggcggttgt cggcggtaag acggtagtaa tcaagcagat agttacagtc ttccacacag
    16561 tagtttttcg ggatcagcga acgggctaaa tcttccgaca gccgttcggt ggtgatcacc
    16621 tgcgtgccgc acggcatgct gcgtttcgct agttccggct ctactttatc gcccagatac
    16681 gcattcccgg cgacaatcac atacttcgcc gtcacctgac cattggcagt ccgaaccacg
    16741 gctggcgtgg tgtgctggat ctgcgtcacg gcggaaagct catacacgcg cccgccgttc
    16801 aggcggatgg catcggcttc accaatggcg aggtttagcg ggtgaatatg cccaccgctg
    16861 tgatccagca gcgcaccagt gtagcgatcg ctggcgacct cgcggcgaat agcgttggcg
    16921 tccagcagct ccagctgttt attgccgtag cgttcccagt tctctttctg ctcttcgagt
    16981 gtggcgagtt gtttatcatt catcgccaca aacagaccgc cggggcggta gtcgcagtca
    17041 atctgataac gtttgatacg ttcgcggatg atctcgccac cctcgaacat catgctgccg
    17101 agcatacggg cggtgtccat gccgtagctt ttttcgatca cgtcgatgtc gcggctgtag
    17161 gagttcacaa gctgcccgcc attgcgcccg cttgcgccaa agccgatgcg tgaggcttcg
    17221 agaaccacta cgtcaaagcc cgcttccgcc agatgcagcg cggaggagag cccggtatag
    17281 ccgccgccaa ccacgcaaac gtcgcaggtg attgactcat tcagtgtgtc gaatggtgca
    17341 tatttattcg cactggcggc gtagtaactg ctggtatgtt cggtcatttc aggcctccag
    17401 gcttatccag atggttttca gttcagtgaa tttttcaagg gcatgcaggg atttgtcgcg
    17461 accgttgccg ctctgcttat agccgccaaa cggcacggtc atatcgccgt cgttgtagtt
    17521 attgacgaag acggaaccgg ctttcaggcg tcggctcatg cggtgcgcgc gggagaggtc
    17581 gcgcgtccat accgccgcgc caaggccgta ctggctgtcg ttggcaagct gcagcgcctg
    17641 atcttctgat gtgaaacggg tgaccaccag caccggaccg aaaatctctt cgcggcttaa
    17701 ggacgcattc gggtccacat ccacgaagat ggtcgggccg atggccgctg ccagcccggc
    17761 gttgcgacca tccagcagca gttgcccctt gccttcgcct tcacgaataa aggtgtgtac
    17821 cgagtcagcg tgagcgctgt cgattaaggt gcccatggtg gttgcgggat caagtggatg
    17881 gcccggctgc cagttttgcg cctgctgttt taacagggct aagaattcat cggcgatgct
    17941 ctcttccagt aacaaacgcg ttccggcgat gcacacctgg ccctggttgt agaagatgcc
    18001 tgcggcggtg gcgcttgccg ctttttgcag atccggacaa tcggcgaaaa cgatgttggc
    18061 gcttttgccg cccgcttcca gccagacacg tttcatgttg ctatcacccg cgtctttcag
    18121 cagttgttta cccgtacgcg tggagccggt aaaggcgatg gcatcgatat cgttatgacg
    18181 cgaaagcgcc tgccccgctt catgaccaaa acccgtcacc acgttcaaca caccatccgg
    18241 caagcctgct tctttcgcca gccccgcgag acgaatcgca ctgagcggtg atttttcaga
    18301 cggttttaga atcacgctgt ttcccgccgc cagcgccggg ccgagtttcc agcaagtcag
    18361 caacagcggg aagttccacg gcacgatggc ggcaatcacg ccgaccggtt cacgcacg
//
LOCUS       DADLSB010000077        18309 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.077, whole genome shotgun sequence.
ACCESSION   DADLSB010000077 DADLSB010000000
VERSION     DADLSB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.077"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            263..490
                     /gene="feoA"
                     /locus_tag="JN331_002456"
     CDS             263..490
                     /gene="feoA"
                     /locus_tag="JN331_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY7260580.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            507..2828
                     /gene="feoB"
                     /locus_tag="JN331_002457"
     CDS             507..2828
                     /gene="feoB"
                     /locus_tag="JN331_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY7260581.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2828..3064
                     /gene="feoC"
                     /locus_tag="JN331_002458"
     CDS             2828..3064
                     /gene="feoC"
                     /locus_tag="JN331_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY7260582.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3267..4145
                     /gene="rpnA"
                     /locus_tag="JN331_002459"
     CDS             3267..4145
                     /gene="rpnA"
                     /locus_tag="JN331_002459"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY7260583.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPEYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMLHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            4218..4487
                     /locus_tag="JN331_002460"
     CDS             4218..4487
                     /locus_tag="JN331_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY7260584.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            4481..4846
                     /locus_tag="JN331_002461"
     CDS             4481..4846
                     /locus_tag="JN331_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY7260585.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VTMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(4847..5635)
                     /gene="bioH"
                     /locus_tag="JN331_002462"
     CDS             complement(4847..5635)
                     /gene="bioH"
                     /locus_tag="JN331_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY7260586.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            5673..6356
                     /gene="gntX"
                     /locus_tag="JN331_002463"
     CDS             5673..6356
                     /gene="gntX"
                     /locus_tag="JN331_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY7260587.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRTTRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            6415..6990
                     /gene="nfuA"
                     /locus_tag="JN331_002464"
     CDS             6415..6990
                     /gene="nfuA"
                     /locus_tag="JN331_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY7260588.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            7351..8667
                     /gene="gntT"
                     /locus_tag="JN331_002465"
     CDS             7351..8667
                     /gene="gntT"
                     /locus_tag="JN331_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7260589.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8712..10796)
                     /gene="malQ"
                     /locus_tag="JN331_002466"
     CDS             complement(8712..10796)
                     /gene="malQ"
                     /locus_tag="JN331_002466"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY7260590.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10806..13199)
                     /gene="malP"
                     /locus_tag="JN331_002467"
     CDS             complement(10806..13199)
                     /gene="malP"
                     /locus_tag="JN331_002467"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY7260591.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVLKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13811..16516
                     /gene="malT"
                     /locus_tag="JN331_002468"
     CDS             13811..16516
                     /gene="malT"
                     /locus_tag="JN331_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAY7260592.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELVAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(16559..17575)
                     /gene="rtcA"
                     /locus_tag="JN331_002469"
     CDS             complement(16559..17575)
                     /gene="rtcA"
                     /locus_tag="JN331_002469"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY7260593.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(17579..>18309)
                     /locus_tag="JN331_002470"
     CDS             complement(17579..>18309)
                     /locus_tag="JN331_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY7260594.1"
                     /translation="NHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQ
                     LETLPSRDLAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQ
                     PQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGL
                     GNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDID
                     AVMAAQSDLVEVIYTLRQVVCVKG"
BASE COUNT         4263 a         5008 c         4612 g         4426 t
ORIGIN      
        1 aaacattagc cagtccgggt aattcactat tcgaattata ttttcgctgc gatataacct
       61 tgagccacat caacattgag tcagattatt attcaaacca acattcgcac acattttaag
      121 tattgctgat agaaaccatt ctcattatca ttgtgttgtt gattatttaa tctctccttt
      181 gttggcaaat catctggtct catgtcgcgg tcaaacgccc catgaggtag ttatccagtt
      241 aatgagaaac aagtaggcac ctatgcaata cactccagat actgcgtgga aaatcactgg
      301 cttttcccgt gaaatcagcc cggcatatcg ccaaaaactg ctttctctag gcatgttacc
      361 tggctcctct tttgatgtgg tgcgcgtcgc tccactcggc gaccccattc atatcgaaac
      421 ccgtcgtgtg agcctggtat tacgcaaaaa agatctggcc ttattagaag tggaagcggt
      481 ttcctgttaa tacggtgata acaacaatga aaaaattaac cattggctta attggtaatc
      541 caaattctgg caagacaacc ttatttaacc agctcactgg cgcacgtcag cgtgtaggta
      601 actgggctgg cgttaccgtc gaacgtaaag aagggcaatt ctccaccacc gatcatcagg
      661 tcacgctggt ggacctgccc ggcacctatt ctctgaccac catctcatcg cagacctcgc
      721 tcgatgagca aatcgcctgt cactacattt tgagtggcga cgccgacctg ctgattaacg
      781 tggtggatgc gtctaacctt gagcgtaacc tgtacctgac gctgcaactg ctggaactcg
      841 gcattccctg catcgtggcg ctgaacatgc tcgacattgc cgagaagcaa aatattcgta
      901 ttgaaattga tgctctatcg gcacgtcttg gctgcccggt gatcccgctg gtttcaaccc
      961 gtggtcgcgg tattgaagcg ctcaagctgg cgattgatcg ctataaagct aacgagaatg
     1021 tggaactggt gcattacgca cagccgctac tcaacgaagc agattcactg gcaaaagtga
     1081 tgccttccga catcccgctg aaacaacgtc gctggctggg cctgcaaatg ctggaaggcg
     1141 atatctacag ccgcgcctac gccggtgaag cgtcgcagca tctggatgcc gccctcgccc
     1201 gtctgcgtaa tgaaatggat gatccggcgc tgcacattgc cgatgcgcgt taccagtgca
     1261 ttgctgccat ctgtgatgtg gtaagcaaca ccctgacggc agaacccagc cgtttcacca
     1321 ctgcggtaga taaaatcgtg ctcaaccgtt tcctcggtct gccgattttc ctctttgtga
     1381 tgtacctgat gttcctgctg gctatcaaca tcggcggggc gttacagccg ctgtttgacg
     1441 tcggctccgt ggcgctattt gtgcatggta ttcaatggat tggctacacg ctccacttcc
     1501 cggactggct gactatcttc ctcgcccagg gcctgggtgg cggtattaac accgtgctgc
     1561 cactggtgcc gcagatcggc atgatgtacc tgttcctctc cttccttgag gactccggct
     1621 atatggcgcg tgcagcgttt gtgatggacc gcctgatgca ggcgctgggc ttgccgggga
     1681 aatcctttgt gccgctgatc gtcggtttcg gttgtaacgt accgtcggta atgggcgcac
     1741 gtacgcttga tgcaccgcgt gaacgtctga tgaccatcat gatggcaccg tttatgtcct
     1801 gcggcgcgcg tctggctatc ttcgcagtat tcgcggctgc cttcttcggg cagaacggtg
     1861 ccctggcggt tttctcgctg tatatgctcg gtatcgtgat ggcggtgctg actggcctga
     1921 tgctcaagta caccatcatg cgcggtgaag cgacgccgtt tgtcatggag ctgccggtct
     1981 accatgtacc acacgttaaa agcctgatta tccagacctg gcagcgtctg aagggcttcg
     2041 ttctgcgtgc tggtaaagtg attatcatcg tcagcatttt cctgagcgct ttcaacagct
     2101 tctcgttgag cgggaaaatc gtcgataaca tcaacgactc ggcgctggct tccgtcagcc
     2161 gggtgataac ccctgtcttc aagccgattg gcgtacatga agataactgg caggcaacgg
     2221 ttggcctgtt tacgggcgca atggcaaaag aagtggtggt gggtacgctc aacaccctct
     2281 acaccgcaga aaatattcag gacgaagagt tcaatccggc ggaattcaac ctcggtgaag
     2341 agctgttcag tgcggtagat gaaacctggc agagcctgaa agacaccttc agccttagcg
     2401 tactgatgaa ccccattgaa gctagcaaag gcgacggtga aatgggtacc ggggcgatgg
     2461 gcgtgatgga tcagaaattc ggtagcgcag ctgccgcata cagctacctg attttcgtcc
     2521 tgctgtatgt accatgtatc tcggtgatgg gagccatcgc ccgtgaatca agccgtggct
     2581 ggatgggctt ctccatcctg tgggggctga atatcgctta ctcactggca acattgttct
     2641 atcaggtcgc cagctacagt cagcacccaa cttacagcct ggtgtgcatt ctggcggtaa
     2701 tcctgtttaa catcgtagtt atcggtctgc tgcgccgcgc gcgtagccgg gtggatatcg
     2761 aactgctggc aacccgcaag tcggtaagca gttgctgcgc agccagcacc accggtgatt
     2821 gccattaatg gcttcactta ttcaggtacg tgatttgctg gcgttacggg gccgtatgga
     2881 agcgacccag ataagccaga cattgaacac tccgcagcca atgattaacg ccatgctgca
     2941 acagctggaa agcatgggca aagccgtgcg gattcaggaa gaacctgacg gctgcctctc
     3001 tggcagttgt aaaagctgcc cggaaggcaa agcctgtctg cgcgagtggt gggcgctgcg
     3061 ttaaccatac tccatcgcct gttttgaaaa gcccggtatg cgtttgcatc cgggcttttt
     3121 tgcgtgcggc tttccataaa aatgcaattc ttgcagcgca acgtaagttc ctctgaaagc
     3181 gtctcgcagg gatgaaaact cgctaatgca cagatgtgga gtggcgcgta gagtcgcggc
     3241 attcaaacaa caggtgaagg aacgccatga gtaaaaagca gagttccacc ccacatgatg
     3301 cgctgttcaa actcttttta cgccaaccgg agacggcacg cgattttctt gcgtttcatt
     3361 taccggcacc cattcacgcg ctctgtgata tgaaaaccct caagctggag tcgagcagct
     3421 ttattgatga cgatctgcgt gaaagctatt ccgatgtgct gtggtcggtg aaaacggaac
     3481 aaggaccaga atacatctat tgtctgattg aacatcaaag cacctcaaac aaactgatcg
     3541 catttcgcat gatgcgttac gctattgccg caatgcaaaa tcaccttgat gctggataca
     3601 aaacgttgcc gatggtggtg ccattgttgt tttaccacgg tattgaaagc ccctatccct
     3661 attcgctgtg ttggctggat tgtttcgccg atcccaaact ggcaaggcag ctttatgcct
     3721 ccgcatttcc gctgattgat gtcaccgtca tgcctgatga tgaaatcatg ctgcaccgac
     3781 gtatggcgct gctggagtta attcaaaaac atattcgtca acgcgatctg atggggctgg
     3841 tagagcaaat ggcctgctta ttaagtagtg gatacgctaa tgacagacaa atcaaagggc
     3901 tgtttaatta catactgcaa actggcgacg ctgtacgttt taacgatttt atcgacggcg
     3961 ttgccgaacg ttcaccgaaa cacaaggaga gtttaatgac tattgcggaa agattgcggc
     4021 aggaggggga acaatccaaa gccctgcata tagccaaaat aatgcttgaa tccggagtcc
     4081 ctcttgcaga catcatgcga tttaccgggc tgtcagaaga agagttggct gcggcgagtc
     4141 agtagatact ctaccctgct taagtgctac tttgtgctat cttgcatgta acacaaagta
     4201 gcacttaggg aactgatatg cagacagtga aattacggca acaaggagga gccatgatcg
     4261 ttactatccc acgagatctt gccatcgatc ttggctggtc accgggcaca gagcttaccg
     4321 tggagaaaaa aggcgatagc gttaatttgc gcgccacaga gcacaaaccg cgtgggcgtc
     4381 ttaccgtcgc gcagttgttg agccaaatcg atgaaagcga aattacggag ctaaatcagt
     4441 ccacagaagg atgggctgaa gggaaaaaag gtaatgaagc atggtaaagg cacggacgcc
     4501 acatcgtggt gagatctggt attttaaccc tgatccggtt gccgggcatg aacttcaggg
     4561 gccacattat tgcattgtgg taacggacaa aaaactcaac aatgttttaa aagttactat
     4621 gtgctgcccg atttcaacag gggcaaatgc agcacgttcc acaggggtga cggtgaacgt
     4681 cctcccccgt gatacgcaaa ccggtaacct gcatggcgtt gtactttgtc accagctaaa
     4741 agccgtcgat cttattgccc gtggcgctaa atttcatacc gttgccgatg aaaaattgat
     4801 tagtgaagtt atcagtaaac tggtgaattt aatcgaccca caataattag ctctcactta
     4861 ccaccaacac cctctgcttc aacgccacca gcaggtgaca aaactcgacc ggatgcgaaa
     4921 taaacggcgc atgggccgct ttggcgaaga tatatgattc gctgtgaggc caaagtttat
     4981 ccagcatcgg caccactttg cgcggcacca gaccgtcgag atagccatac aatcgcaaaa
     5041 acggcatgga cacgttttgc agcggctgac ggagatcgac cgttttcagg atttccagcc
     5101 cgccattaag cacgtcaacc tccggcatcg gtaacgccag aacggttttc ttcaacgccc
     5161 gcgcatcctg gcgcgccgtt tcagtcccca tggtttgtaa cgccaggaac cgctccactg
     5221 tacgctgaaa atcatcactg agttgctgct gaaatcccgc cagcacgtcc ggttttatcc
     5281 ccggccactc gtcacgagca ctaaaacaag gtgacgacgc cacggtgacc agcgcctgaa
     5341 cacgctcggg atgggttaac gcaatctggc ttgccaccag cccgcccaga ctccagccta
     5401 accaaatggc tttatcaggt gcctgttgca gcacggcttc ggccatatca gcaagtgaca
     5461 gcgcaccaaa tccccggcta cgcccgaagc cgggcaggtc aacaaggtgc agcgtaaaat
     5521 gcgagctaag ttcctcgtca atgcaacgcc acacttcggc attcagtccc catccgtgca
     5581 gcagcacaag atgaacattc ccctgacctt tggtctgcca ccagatgtta ttcatccgct
     5641 attgttctct tttgacttac aaggatgaac atatgctaac agtaccggga ttatgctggc
     5701 tatgccgaat gccactggcg ttaggtcatt gggggatttg ttcggtctgt tcacgcacca
     5761 cccgcacaga taaaacgtta tgcccacaat gtggattacc cgccacacac tcccatcttc
     5821 cctgcggtcg ctgcctgcaa aaaccgccgc cctggcaaag actggtcacg gttgccgact
     5881 atgcgccgcc gttaagtccg cttatccacc agcttaaatt ttcccggcgc agtgaaatcg
     5941 ccagcgccct gtcacgtctg ttactattgg aagtcttaca cgctcgtcgc accaccgggt
     6001 tgcaattgcc ggatcgcatc atcagcgttc cgttatggca gcggcgtcac tggcgtcggg
     6061 gatttaatca gagcgatttg ctgtgtcagc cgttatcacg ctggttgcac tgccaatggg
     6121 atagcgaagc cgtcacacgt acacgggcca ctgcgaccca gcattttctc agtgcccggc
     6181 tgcgcaagcg caacctgaaa aatgcctttc gtcttgaatt gcccgtgcaa ggtcgccata
     6241 tggtgattgt ggatgatgtc gttaccaccg gaagtaccgt cgcagagatt gcgcagttgc
     6301 ttttacgcaa tggtgcggcg actgtccagg tctggtgcct ttgtcgaacc ttgtagagcc
     6361 tcgatgatgg gcgtattata accaactaaa atagtcaact attaggccat tactatgatc
     6421 cgtatttccg atgctgcaca agcgcacttt gccaaactgc tggcaaatca ggaagaaggg
     6481 acacaaatcc gcgtatttgt gattaaccct ggcacgccta acgctgaatg tggtgtttct
     6541 tattgtccgc cggatgccgt ggaagccacc gacactgccc tgaaatttga cctgctgacc
     6601 gcgtatgttg atgagttaag cgcaccatac ctggaagatg cagagatcga ctttgttacc
     6661 gaccagttgg gttctcagtt aacgctgaaa gccccgaacg ccaaaatgcg taaagtggca
     6721 gacgatgcac cgctgatgga gcgcgtggag tatatgctgc agtcgcagat caacccacag
     6781 cttgctggtc acggtggtcg cgtttcgctg atggaaatca ccgaagacgg ttacgccatt
     6841 ctgcaatttg gcggcggctg taacggttgt tccatggtcg atgtgacgct gaaagaaggg
     6901 atcgagaagc agctgctgaa cgaattcccg gagctgaaag gtgtgcgcga tctcaccgaa
     6961 caccagcgcg gcgaacactc ctactactaa gttatcccct cttttgtaga ttgcccgatg
     7021 cgacgctaaa gcgtcatttc gggcctacgt tgaacatgtg ccagacgttg gcacagcatt
     7081 gctttggcaa atccctccct tcgtttcccc gcgataatat gaccaacctc tcataattta
     7141 aatttacccc gctctggtga ttctcaaacg ccagatgtta cccgtatcat tcacatgggt
     7201 accaaacata ctcctgacat ctgactacaa taattagttt tagtgggtat cagtcgtggt
     7261 gccgcaatat ctctgttccc gattgggata attagagttt gtcgtcagaa aattgacgtt
     7321 acccataaca aatgaaaggc caggtaaatc atgccattag tcattgttgc tatcggtgta
     7381 atcttgttgt tgctcctgat gatccgcttc aaaatgaacg gcttcatcgc tctcgtcctc
     7441 gtggcgcttg ctgttggatt aatgcaagga atgccgctgg ataaagttat tggctccatc
     7501 aaagccggtg tcggcgggac gctcggtagc cttgccctga tcatgggttt tggcgcaatg
     7561 ctgggcaaaa tgctggcaga ctgcggtggc gcacaacgta tcgccaccac gctgattgcc
     7621 aaatttggta aaaagcacat ccagtgggcg gtggtgttaa ccggttttac cgttggtttt
     7681 gccctgttct atgaagtggg cttcgtgctg atgctgccgc tggtgtttac catcgcggct
     7741 tccgcgaata ttccattgct gtatgttggt gtaccaatgg cggctgcact gtctgtgacc
     7801 cacggcttcc tgccaccgca tccgggcccg actgcgattg ccaccatttt caatgccgat
     7861 atgggtaaaa ccctgctgta cggtactatt ctggcaatcc cgaccgtgat tctcgccggt
     7921 ccggtttacg ctcgcgtgtt gaaaggtatc gataagccaa tcccggaagg tctctacagc
     7981 gcgaaaacct tcagcgaaga agagatgccg agctttggcg tcagcgtctg gacctctctg
     8041 gtgccggttg tgctgatggc gatgcgtgcg attgccgaaa tgatcctgcc gaaaggtcac
     8101 gctttcctgc cggtagcgga gttcctcggt gacccggtaa tggcaacgct gattgccgtg
     8161 ctgattgcga tgttcacctt tggtctgaac cgtggtcgtt caatggatca gattaacgac
     8221 acgctggttt cttccatcaa aatcattgcg atgatgctgt tgatcatcgg tggtggcggt
     8281 gcgttcaagc aggtgctggt agacagcggc gtggacaaat acattgcttc catgatgcac
     8341 gaaaccaaca tttctccgct gctgatggcc tggtcgattg ctgccgtact gcgtatcgcg
     8401 ctgggttccg caaccgttgc ggcaatcact gcgggtggta tcgcggcacc gctgattgca
     8461 acgacgggtg ttagcccaga gctgatggtt attgcggtgg gttccggtag tgtgattttc
     8521 tctcatgtga acgatccggg cttctggctg ttcaaagagt actttaacct gactatcggc
     8581 gagaccatca aatcctggtc gatgctggaa accattatct cggtgtgcgg tctggtaggc
     8641 tgtctgctgc tgaatatggt gatttgataa cactgactgc cggatgcatc gcatccggca
     8701 tcaaccgcac tctacttctt cttcgctgca gctctgcgcc gtctgtccaa atccttcagc
     8761 aacttgttca cgccatcatc ggcaaacatc gactcaagcg ttgcggaaag cttgcgtcgc
     8821 cagtttttat actggtaact ggtgccagga atattcaccg gttcggccat atccagccag
     8881 tcttccggct gtagtcctaa cagagcactg ttactgtcgg caatgtagcg ctgcaaacca
     8941 cggttcagcg tcggcgtcat cgacatcaac gatgccttat gcccggcacg ttttggcaga
     9001 caaccatatt tatgcagtgc atccagcagc ccttgcttcg ccagttcgcg atcctgatac
     9061 agaccgcgca gtaccacttc atccggatac agccccaggg ttttgcccag cgttagatcc
     9121 ccgctctccc agtaaccacg cagcgttggc aggtcatgtg tcgccgcaac cgccatcgac
     9181 tgctccggat acgctttcgg tgcacggaac gtcttctcgt ggtcgttttc gaaatagagc
     9241 actttgtaag agtacacacc gctgctgcgc agcttaccga caatctctac cggtacggta
     9301 ccgagatctt caccaatcac catacagcga tgacgtttac tttcgagtgc cagaatcgag
     9361 agcagatcat ccaccggata gtgaacatac gcgccctgat ctgccgtctc gccatacggt
     9421 atccaccaca aacgcagcat cgacatcaca tggtcaattc gtaatgcgcc gcagttttgc
     9481 atattggcac gcaacagctc gataaacggt tcataggcac gcgcggtgat gatatgcggg
     9541 tccattggcg gtaatcccca gttctgcccc aacgggccga ggatatccgg cggcgcgcca
     9601 accgatgctt tcaggcaata tagttcacgg tcacaccagg tttccgcccc accttccgct
     9661 acgccaaccg ccagatcacg atacaagcca atcggcattt catagccctg gcttatctcc
     9721 cagcaggcgg caaactggct gtaagccagc cactgcaacc agagataaaa atcgacgtca
     9781 tcacgatgtt cttcgcagaa ctgacgcact tctggtgaat ccacgttctt atacatctct
     9841 ggccatgcag gccagcccca gcgcatttcg tcctctttca cttgctgggc atgtagcgca
     9901 tcaaaggctg cctgccagaa caggctgtcg ccctgctctg caacaaactg gcgaaacgcg
     9961 gccatctgct catcatcacg ttgcgcgaaa cctttccacg ccattcgtaa tgctgtcatt
    10021 tttagggcgg taaccgtgga gtaatcgacc cagtcggcat cgcgcgcctg ttgcagcgtc
    10081 tgttgcgtgg tcggcaactg ccaccaggcc tgagcctctt cgctaagatg gaaatcttca
    10141 acggcgttaa cgtcgatata aatcacattc agccaacggc gagaagacgg gctgtatggg
    10201 ctggcgctct ccggatttgc cggatagagc gcatgaatcg ggttcaggcc aatgaacgac
    10261 ccgccacgtt ttgccacatc caccagcatc gctttgagat cgccaaaatc cccaataccc
    10321 cagttttttt ccgatcgcag cgtataaagc tgaacgcagg ccccccacag cttttgttta
    10381 ttcagcaaag cctgcggttc gtaacaacgt ttcggggcga caatcacccg gcaatgcgcg
    10441 cgctggtcgt cctgggtgag tgttagcgtg tgataacctt ccggcagctt cgtcggtaga
    10501 ttgaacgctt tgccccccgt tacatggcct ttgtactgcg ttccttcttc ggtggtcagc
    10561 tgccagctat attcgccgct gccctccacc accatcggca tttttttgcc gctggtataa
    10621 accatgacat tcgggactgg cgttaccgcc actttcgtgg cggtacgttg atgcatcgcg
    10681 tcaagcaaac gccgtttggt ttcggcgcta atcgactgcg gtttaccgtg ggcattgatg
    10741 taattggggc taatccccgc cgccagcgcg gcattatcca gacgtttgct ttccatcgag
    10801 cttccttagc gttttgcctg ccagatacga gcctgataat cgcgaataga gcgatccgag
    10861 ctaaacatac cgcagcgggc ggtattgagg atcgccgcgc gagtccaggc ctcctggtcg
    10921 cggtacagca catccacctg cttttgtgcc tcaacatagg ctgcgaaatc cgccatcacc
    10981 agatacggat cgccgccctg tttgccgata ctgtgcagca tctggtcgaa ggcatgctta
    11041 tcgccgtcgc tgtatttacc gctttccagc tctttcaata ctgcgtccag caccttatct
    11101 ttcttccgcc atttcaccgg gtcgtagcct ttggccagaa tagccttcac ttgttccacg
    11161 gtatgaccaa aaataaagat attttcttca ccgactttct cggcgatttc aacgttcgca
    11221 ccatccagcg taccgacagt aagcgcacca ttgagcgcca gtttcatatt gccggtaccg
    11281 gaagcttctt tacctgcagt cgaaatttgt tcggagatat ccgccgccgg gatcagtttt
    11341 tccgccgccg aaacgcaata atccggcagg aacaccacct tcaacttatc gccaaccagt
    11401 ggatcgttgt tgatcacgtc agccactttg ttgatcgcaa agataatatt cttcgccagg
    11461 tagtagcccg gtgccgcttt cgcgccgaag aggaagacgc gcggtacgcg atcagcctgc
    11521 gggttttcac gaatttcttt gtacaacgcc agaatatgca gcagattcag gtgctggcgt
    11581 ttgtactcat gcagacgttt gatctgaata tcgaaaatcg cctgtggatt gatctcaata
    11641 ccggtacgaa ctttcacaaa ctccgccaga cggactttat tcgcctgctt gatctcgcga
    11701 tattgctgac ggaatttcgc atcatcagcg aatttttcca gattgatcag ctgatcgaga
    11761 tcgttagccc actctttttg cagtgattta tccaacagag ccgccagtgc agggttgcac
    11821 tgtttgatcc agcgacgtgg ggtaataccg ttggtgacgt tatggaattt gttcggccat
    11881 agctggtgat attccgggaa cagatctttc aacaccagat ccgagtgcag cgccgcaaca
    11941 ccgttcaccg cgaaaccgcc aaccacacac aggttcgcca tatgcacttg tttatcgtgc
    12001 accaccgcca gtttggccca cactttttca tcgcccggcc aggttttctc caccagcgtt
    12061 ttaaagcgag tattaatttc gttaataatc tgcatatggc gcggtagtaa gcctttcacc
    12121 agtttcacat cccagcgttc cagcgcttct ggcatcaggg tatggttggt gtaagcgaaa
    12181 gttttgctgg tgatggccca ggcgtcatcc cagctcatct ggtgctcatc gatcagcacg
    12241 cgcagcagtt ctggaatcgc gatagttgga tgggtatcgt tcagctgaat aacttcgtaa
    12301 tccgccagtt cgtgcagttt acgccccgcc agatgatggc gacgcaaaat atccgctacc
    12361 gaacaggcac actggaagta ttgctgcatc aggcgcagct ttttaccggc agtatggttg
    12421 tcgtttggat agagaacttt ggtcagtttt tccgcattga tgccttgctg ttcggcgcgc
    12481 aggaaatcac cgtcgttaaa tttagttaga tcaaacggat gcgcatgcgt cgcctgccac
    12541 agacgcagcg gctgcgccac gccattacga tagccgacaa cggggagatc ccacgcttga
    12601 ccggtaatgg taaactccgg ctcccagcgt ccgtctttca tcactttacc gccaatccct
    12661 acctgcacat ccagtgcttc gttgtggcgg aaccacgggt agttactgcg atgccagtca
    12721 tccggcgctt caacctgttt gccatcgaca aaagactggc ggaacaaacc atattgatag
    12781 ttcagaccgt aacccgtcgc agactgaccg acagttgcca ttgagtcgag gaagcacgcc
    12841 gccagacgtc ccagaccacc gttacccagc gccgggtcga tctcttcttc cagcaggtca
    12901 gtcaggttga tgtcataagc cttcaacgaa tcctgtacat cctgatacca gccgagattc
    12961 aacaggttgt tgcccgtcag acgaccaatc aaaaactcca ttgagatgta gttaacatgt
    13021 cgctgattcg ccaccggttt ggcgaatggc tgagcacgca gcatttcggc cagtgcttca
    13081 ctcactgcca gccaccactg gcgaggagtc atttcagccg cagaatttaa gccataacgc
    13141 tgccactgac gtgaaagcgc ttcctgaaat tgcttatcgt taaaaatagg ttgtgacata
    13201 ggagttccac ttttcttaga ttttcaacac aacgttatcg ctagtttgcc aggctcgatg
    13261 ttgaccttcc tcatcctgcg ggggattagg cagggaggag ttgcggggat gagcaaggaa
    13321 atgtgatctc gaccacttaa agctagtgca aaccacagga ttagcagcaa atcaatgcca
    13381 tacagcgcag aaaatctgta tctaagtgca aaaaatggcc gttgcgtatt ttcaaaaagc
    13441 ggaaggtaac tctataaatt aagtaaagga gtgaaacagt ttcacaagta aaatatccag
    13501 tgtgctccat ctcattctta atagatttat taagatcatc tttttagatg gcactttcat
    13561 caggaatgaa gaagaaaccc ttgcttaaat gaatctgatg aacataaggg aaaccagtat
    13621 tcacgctgga tcagcgtcgt tttaggtgag ttgttaataa agatttggaa ttgtgacaca
    13681 gtgcaaattc agacacataa aaaaacgtca tcgcttgcat tagaaaggtt tctggccgac
    13741 cttataacca ttaattacga agcgcaaaaa aaataatatt tcctcatttt ccacagtgaa
    13801 gtgattaact atgctgattc cgtcaaaact aagtcgtccg gttcgactcg accataccgt
    13861 ggttcgtgag cgcctgctgg ctaaactttc cggcgcgaac aacttccggc tggcgctgat
    13921 cacgagtcct gcgggctacg gaaagaccac cctcatttcc cagtgggcgg caggcaaaaa
    13981 cgatatcggc tggtactcgc tggatgaagg tgataaccag caagagcgtt tcgccagcta
    14041 tctcattgcc gccgtgcagc aggcaaccaa cggtcactgt gcgatatgtg agacgatggc
    14101 gcaaaaacgg caatatgcca gcctgacgtc actcttcgcc cagcttttca ttgagctggc
    14161 ggaatggcat agcccacttt atctggtcat cgatgactat catctgatca ctaatccagt
    14221 gatccacgag tcaatgcgct tctttattcg ccatcaacca gaaaatctca ccctggtggt
    14281 gttgtcacgc aaccttccgc aactgggcat tgccaatctg cgtgttcgtg accaactgct
    14341 ggaaattggc agtcagcaac tggcatttac ccatcaggaa gcgaagcagt tttttgattg
    14401 ccgtctgtca tcgccgattg aagccgcaga aagcagtcgg atttgcgatg acgtttccgg
    14461 ttgggcgacg gcactacagc taatcgccct ctccgcccgg cagaataccc actcagccca
    14521 taagtcggca cgccgcctgg cgggaatcaa tgccagccat ctttcggatt atctggtcga
    14581 tgaggttttg gataacgtcg atctcgcaac gcgccatttt ctgttgaaaa gcgccatttt
    14641 gcgctcaatg aacgatgccc tcatcacccg tgtgaccggc gaagaaaacg ggcaaatgcg
    14701 tctcgaagag attgagcgtc aggggctgtt tttacagcgg atggatgata ccggcgagtg
    14761 gttctgctat cacccgctgt ttggcaactt cctgcgccag cgctgccagt gggaactggt
    14821 ggcggagctg ccggaaatcc accgtgccgc cgcagaaagc tggatggccc agggatttcc
    14881 cagcgaagcg attcatcatg cgctggcggc aggcgatgcg ctgatgctgc gcgatattct
    14941 gcttaatcac gcctggagtt tgttcaacca tagcgaactg tcgctgctgg aagagtcgct
    15001 taaggccctg ccgtgggaca gtttgctgga aaatccgcag ttagtgttat tgcaggcgtg
    15061 gctaatgcaa agccaacatc gctatggcga agttaacacc ctgctagccc gtgctgaaca
    15121 tgaaatcaag gacatcagag aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca
    15181 ggtggcgatt aacgatggta atccggatga agcggaacgg ctggcaaaac tggcactgga
    15241 agagctgccg ccgggctggt tctatagccg cattgtggca acctcggtgc tgggtgaagt
    15301 gctgcactgc aaaggcgaat tgacccgctc actggcgcta atgcagcaaa ccgaacagat
    15361 ggcacgccag cacgacgtct ggcactacgc cttatggagt ttaattcagc aaagtgaaat
    15421 tctgtttgcc caagggttcc tgcaaaccgc gtgggaaacg caggaaaaag cgttccagct
    15481 gatcaacgag cagcatctgg aacagctgcc aatgcatgag tttctggtgc gcatccgagc
    15541 acaactgtta tgggcctggg cacggctgga tgaagccgaa gcgtcggcgc gtagcgggat
    15601 tgaagtcttg tcgtcttatc agccacagca acagcttcag tgcctggcaa tgttgattca
    15661 atgctcgctg gcccgtggtg atttagataa cgcccgtagc cagttgaacc gtctggaaaa
    15721 cctgctgggg aatggcaaat atcacagcga ctggatctct aacgccaaca aagtccgggt
    15781 gatttactgg caaatgaccg gcgataaagc cgccgctgcc aactggttgc gtcatacggc
    15841 taaaccggag tttgcgaaca accacttcct gcaaggtcaa tggcgcaaca ttgcccgcgc
    15901 acaaatcttg ctgggcgagt ttgaacctgc ggaaattgtt ctcgaagaac tcaatgaaaa
    15961 tgcccgcagt ctgcggttga tgagcgatct caaccgtaac ctgttgctgc ttaatcaact
    16021 gtactggcag gccggacgta aaagtgacgc ccagcgcgtg ttgctggacg cattaaaact
    16081 ggcgaatcgc accggattta tcagtcattt tgtcatcgaa ggcgaagcga tggcgcaaca
    16141 actgcgccag ctgattcaac ttaatacgct gccggaactg gaacagcatc gcgcgcagcg
    16201 tattctgcga gaaatcaatc aacatcatcg gcataaattc gcccatttcg atgagaattt
    16261 cgttgaacgt ctgctaaatc atccggaagt gccagaacta atccgcacca gcccgttgac
    16321 ccaacgtgaa tggcaggtcc tggggctgat ctactccggt tacagcaatg aacaaattgc
    16381 gggcgaactg gaagtggcgg caaccaccat caagacgcat atccgcaatc tgtaccaaaa
    16441 actcggcgtc gcccaccgtc aggctgccgt gcagcacgcg cagaaactgc tgaagatgat
    16501 gggttatggg gtgtgagttt agccggataa tgcattggat ccggtttaca tctttacatc
    16561 attcaatact cacccgcgtt acgccatctg tttctatcaa accaaaccgc accggcaaga
    16621 aacgctccac caccgcgata ttggtcagca gatggcatga aggatgggcg accgtaaatt
    16681 ctcccgcgcc cgccagcgcc atcggcagta ccagttggtc ggcgagatat tcccccaccg
    16741 ccgctgggct tgccaggtag cgtttcacct ctttcactaa ctgcgcggct accacctcgg
    16801 cactgacgcg cttttcaccg acgacgaaaa agcgttcagt gatattttca ctttcgactt
    16861 ccagcgagac ggtattaccc ggcccctggt cgcgcggcag gttatgaata ttctgttcat
    16921 gcagggaaaa actccccgcc agtgtagcga tttcacgctc agcaacatgg cgcggcacac
    16981 cagctaatag aacttctcca cgcatctgca caatgttccc gcgctcgcca agttgcaagg
    17041 tgttaaacga tgccacgggc gagacttccg ttgccaccac accgcctccg gcaggataaa
    17101 aaccgtgacg taacagcgtg gtttgctgat gaattcctat tttcgccagc agcggctcca
    17161 gcacccggcg gataaaatcc gcaggcggcg ccgacgggtt atcggtgccg ccgctcactt
    17221 caacacgcga aggtccatcg gcaaaccaca gcgcgggcag caccgtttgc agcaccagcg
    17281 tacaacttcc ggcgctaccg atagcaaagc ggtaatcgcc gccgcgcacg gtgccaggcc
    17341 ggaagaccag acgctgcgat cccagctccg cgccttccac cgttgcccta caaatttccg
    17401 cagccgcttt taccgcggtc agatgctggc gcagcagccc cggtttcgcc cgtccggctc
    17461 gaatgccagt gatggtaaat ggctggccgg ttatcatcga caggctcagc gccgagcgca
    17521 ggatctgccc gccgccttcg ccctgtgcgc catccagcgc aatcatcctt ttcatcattt
    17581 atccttttac gcacaccacc tgacgcaggg tgtagataac ttccaccaga tcgctttgtg
    17641 ccgccatcac cgcatcaata tctttatacg ccatcgggat ttcgtcgatc acttcggcat
    17701 ctttacggca ttccacatgc gcggtggcac gaatttggtc ttccacgctg aacagttttt
    17761 tcgctttagt tcggctcatt acccgcccgg caccgtggct gcacgaacag aacgactctt
    17821 catttcccag cccacggacg ataaagcttt ttgctcccat cgaaccggga ataattccat
    17881 attgaccagc acgcgcagac accgcacctt tacgcgtcac gtagatctct tcaccaaagt
    17941 gctgttcttt ttgcacatag ttgtggtgac agttgatctc ttccatcgcc agcgtttgtg
    18001 gctgtctgac cgttttctgc gtaatgctct gcaacgccgt taccacattt tccatcatcg
    18061 catcgcggtt aaggctggcg aaaagctgcg cccaggccac ggctcttagg taatcatcaa
    18121 agtattccgt accttccata aagtacgcca gatcacgcga cggcaacgtc tcaagttggt
    18181 cctgcatctc tttttgcgcc agatcgataa agtaagtccc gatggcgtta ccaattccgc
    18241 gtgaaccgga gtgcagcata atccacacct gctccgactc atcaaggcag atttcaataa
    18301 agtggttac
//
LOCUS       DADLSB010000078        18204 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.078, whole genome shotgun sequence.
ACCESSION   DADLSB010000078 DADLSB010000000
VERSION     DADLSB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.078"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            481..1137
                     /gene="pphA"
                     /locus_tag="JN331_002471"
     CDS             481..1137
                     /gene="pphA"
                     /locus_tag="JN331_002471"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY7260595.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIVLADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1138..1413)
                     /locus_tag="JN331_002472"
     CDS             complement(1138..1413)
                     /locus_tag="JN331_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY7260596.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(1434..1670)
                     /locus_tag="JN331_002473"
     CDS             complement(1434..1670)
                     /locus_tag="JN331_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY7260597.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1788..3227)
                     /gene="rsmF"
                     /locus_tag="JN331_002474"
     CDS             complement(1788..3227)
                     /gene="rsmF"
                     /locus_tag="JN331_002474"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY7260598.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(3307..5940)
                     /gene="yebT"
                     /locus_tag="JN331_002475"
     CDS             complement(3307..5940)
                     /gene="yebT"
                     /locus_tag="JN331_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAY7260599.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDALIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5909..7192)
                     /gene="yebS"
                     /locus_tag="JN331_002476"
     CDS             complement(5909..7192)
                     /gene="yebS"
                     /locus_tag="JN331_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAY7260600.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAE
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7322..7819
                     /locus_tag="JN331_002477"
     CDS             7322..7819
                     /locus_tag="JN331_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY7260601.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7916..8614
                     /gene="proQ"
                     /locus_tag="JN331_002478"
     CDS             7916..8614
                     /gene="proQ"
                     /locus_tag="JN331_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY7260602.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8634..10682
                     /gene="prc"
                     /locus_tag="JN331_002479"
     CDS             8634..10682
                     /gene="prc"
                     /locus_tag="JN331_002479"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY7260603.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQTAPVK
                     "
     gene            10874..11755
                     /gene="htpX"
                     /locus_tag="JN331_002480"
     CDS             10874..11755
                     /gene="htpX"
                     /locus_tag="JN331_002480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY7260604.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11801..13174)
                     /locus_tag="JN331_002481"
     CDS             complement(11801..13174)
                     /locus_tag="JN331_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7260605.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            13351..14142
                     /gene="kdgR"
                     /locus_tag="JN331_002482"
     CDS             13351..14142
                     /gene="kdgR"
                     /locus_tag="JN331_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY7260606.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14285..14524)
                     /locus_tag="JN331_002483"
     CDS             complement(14285..14524)
                     /locus_tag="JN331_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260607.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14683..14826
                     /gene="mgrB"
                     /locus_tag="JN331_002484"
     CDS             14683..14826
                     /gene="mgrB"
                     /locus_tag="JN331_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY7260608.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14901..15188
                     /locus_tag="JN331_002485"
     CDS             14901..15188
                     /locus_tag="JN331_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260609.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            15858..16001
                     /locus_tag="JN331_002486"
     CDS             15858..16001
                     /locus_tag="JN331_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY7260610.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            16014..16223
                     /gene="cspE"
                     /locus_tag="JN331_002487"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             16014..16223
                     /gene="cspE"
                     /locus_tag="JN331_002487"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7260611.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            16389..17198
                     /gene="rlmA"
                     /locus_tag="JN331_002488"
     CDS             16389..17198
                     /gene="rlmA"
                     /locus_tag="JN331_002488"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY7260612.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPEPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(17195..17761)
                     /gene="mntP"
                     /locus_tag="JN331_002489"
     CDS             complement(17195..17761)
                     /gene="mntP"
                     /locus_tag="JN331_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY7260613.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPHRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4822 a         4347 c         4584 g         4451 t
ORIGIN      
        1 cccaaatcgt aaaggaaacc gtagcacgtc gtatgcaaga acgtgccacg gctggctgat
       61 ggatgttcga tagcgcgagt ttgaatgaaa atcagccgga gatgatttta cataattgct
      121 acggaattat tcaatacagg aattgcttgc gtctgcatgg attgacctga aatattcccg
      181 aaaatttctc taaaaaactc gaaaaaaatg gtaactggtt gaatgtatta atatgcaatg
      241 gtacgtgtca gggattaaaa gatgaacgta aatttattca acgcattaat tttaaagggt
      301 tttattgttt gttgacgaaa acaggaatcg tgttcggtct ctttttatct gttaaaagcc
      361 agaagcattt ccttcgctga ctttatagtc aaccataaca cacactctac tgtctgagtc
      421 cagcgttttt taacattctt gttaagatta tgtgatcttt agcgcgggag gaaaatattg
      481 atgaaacagc ctgcgcccgt ttatcagaga attgcgggtc atcaatggcg acatatctgg
      541 ctttctggcg atatacacgg ttgtcttgag cagttgcgcc gcaaattatg gcattgtcgt
      601 tttgatccgt ggcgagattt acttatctca gtgggagacg ttatcgatcg tgggccgcaa
      661 agtttacgtt gtttgcagtt actggaacaa cattgggttc gtgcggtaag aggcaatcat
      721 gaacagatgg cgatggatgc gctggcattc cagcagatgt ctttgtggtt gatgaatggc
      781 ggcgactggt ttattgtgct ggcagataat caacagaaac aagcgaaaac ggcgctggaa
      841 aaatgtcagc atttgccctt tattcttgaa gtacacagtc gcaccggcaa gcatgttatt
      901 gctcatgccg attatccaga tgatgtttat gaatggcaaa aggacgttga tttgcatcag
      961 gtcttgtgga gccgctcgcg attaggtgaa cgcccaaaag ggcagggaat tacaggtgct
     1021 gatcatttct ggtttggtca tacaccgttg cgacatcgcg tggatattgg caacctgcat
     1081 tatattgata ccggtgctgt ctttgggggc gaattgactc ttgtgcaact gcaataatta
     1141 aaaatcacca tactcctgtg ctggtcgcca gaaaccatct ataaaatcct caatcggaaa
     1201 acaaccgcca tggcggatcc gttgatcgct catagaataa agacactgct gttccgtgtt
     1261 gtagacatcc acaacaatat cttcacaacc gccatccagg tagcaaacaa aaagtaccag
     1321 cgcgaacatt tcatccccga agtgtggtgc cgtaccgtta agtttaggag agattttaca
     1381 acgggggaat aaccaggaca aataacccgc catgccgacg ggttattttt ggatcaggca
     1441 agacgcataa tccaggcatc agattgttga tcgtaacggg tacggtaaag gacagagtgt
     1501 tcgccattta gcagggcagg gatactggtt tcagcatccc aggcatccag ttgcatacat
     1561 aaatcggggt cagatttaca aggaatggtt aacgttcgat cacccggcga ttcaccgtgt
     1621 aattcgccgt cgtcgatttc aaaagcgcct atgcgcacac tggttttcat cttcgtgctc
     1681 tccatttagc tgccgattgt ggtacgacca gtccgatcgc acaatttttc agcctagttg
     1741 aggccgccga atgtgccagt caaaaagtgc gctttttttg tgcgctgtca tgcgttactg
     1801 gtaaaaagtt tcccgtcccg caccagttca cgcgggtagc tgtttttcag tcgcgaacca
     1861 atccgtttgg ctaaaccaat cggctgatgc tggaaagtaa ccaacacgtc atccgccact
     1921 ggcgcggctt gcgggtaaac atcgcgcccg cgataccact cctccgcttc ctgcggtgtc
     1981 agttcaaaag cgtttacatt gtcgggggag gcatgggcaa taaccgcttc atgctgccag
     2041 cgataacctt tgttatgcgt ttcggcaagt ttaatcccca agcgagaaaa tcggacttta
     2101 ccgatcaggg cttcaatgcc taccgggaac aaccacagtt ctttgtcgcg ttgccagagt
     2161 cgcaggtttc catcccagtt taagccaaca cttgcagccg cctgacgaat ttgtccggct
     2221 tcgcgatctt tcaccgggct gaacgggaaa ttgcccactt tgtattttgg ggcgggtaag
     2281 gcggggatcg cctgagtttt acgcagacga gcaacgaaga agccttcgca gtcgtaaatt
     2341 tgtgggaaaa catgcaaaaa accttcttcg gtcagcgcct tgtttgcacc agggaagaga
     2401 tcgccaagtg gtaaaaactc tacttcgtcg gggtaagtct ctttcagcca ccggcaaacg
     2461 gcttcgtttt cttcccggtt taaggtacag gtcgagtaaa ccagcgtacc gccaggacgt
     2521 aatgcatgaa aggcgctgtc aatcagttcc cgttgggtag ctgcgatttc ctgattgctt
     2581 tcgggtgacc agttttttag cgcatcggga tctttacgca ccacgccttc gccggagcag
     2641 ggagcgtcca gcaaaatggc atcaaacatt tctggcactg ccgcaccaaa cacgcggcca
     2701 tcaaaatgtg tgagcgcaac attactgatg ccacagcggc tgatattggc atgtaacact
     2761 tttacccgac tggcggaaaa ctcattggca aggattgcac cttcgttatt catccgcgcg
     2821 gcaatttgcg tcgtttttga gcctggcgca gcagcgacat ccatcacccg ctgtggtgca
     2881 ttaccgtcag caaacaaggc ggcgacgggc aacattgaac tggcttcctg aatataaaac
     2941 aggccactta aatgctcggt ggtactaccc aatggcaatg catcttcatt gtcgcgttca
     3001 atccagaaac cttcttcaca ccacggaatt ggcgtaagcg tccagccata aggagcggtt
     3061 aattgcagga aatcagcaac ggagattttc agcgtattaa cgcgaatgct gcggcgcaac
     3121 gggcgctgac aggcggcaag aaaatcatca aatgagagcg tcgaaggcat agcttcgcgc
     3181 atttgtgtca gaaacgcgtc cgggaaataa acggtgtgtt gggccacgag catgtaccac
     3241 tggcaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac gccggagcag
     3301 tgggcattat ttgggaagcg cagttcccca ttcacgccac tctttcggtt cactttcctg
     3361 caacaggaag tgtttgcctt cctgggcttt cggtgcaagt ggcgtacccg gaggcgtggc
     3421 gaaggcgatg ccgccacgga taaattggtt aaaggtgccg gttttcacta cgccgcccgt
     3481 cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt tacgcaccag
     3541 gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca atgtccccag
     3601 cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg taccgatgcc
     3661 taacgaaccg gcttccggcg cttcaacaat aatgcttaaa ccatctaggt aacgcgaatc
     3721 agtaatggtg gcctcttgta attcaaagtc gcgacgagga ttgccacggc ctggttcgac
     3781 gttgatatac ggctggagga tagtatctag atgctcaacg ccagctgccg agatttgcgg
     3841 tgtgaccact gagaagcgcg taccaccgcg agcaaaggtc tggacatatt ccggatagag
     3901 caccgccttt gcctgtactt cattgcgtgc ggtaatcaga tccagcgtct ggatttgccc
     3961 gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg catcgaaagc
     4021 gtgaagcgta atctgcccgc caaccgcacg ggccgctgtt tcggaagcat acagaattcg
     4081 cttgtcgcct ttacgctgac tggcgctggc accactgagg ttatcgaagc taatggcccc
     4141 ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat tcagctgaac
     4201 tttcgccccg ccttctgccc agaacacgct gttgctggtc aaaaggttgc gatattccgg
     4261 tttaatgtgc agatcgatat cgaacgcgtt agctcgcgga cgcacggtaa taacttcacc
     4321 aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca gcgtctctgc
     4381 actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg ctttttccag
     4441 attggcatac agtggatagc tggctttcat ctcgccttta tcgcccggca gaatacgtat
     4501 cccgccgtta atccattctg aggcgctggc accgagaaac tcaacgccat ccagccccac
     4561 cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca gttctcgatg
     4621 ctgaggctcg atggcgacgg taaaggtgac gcctttgctg gtgagtttac gatcgataac
     4681 ctggcctacc tgcacgccgt gaagaatgag cggctgaccc gcatcaatac cgtaactttc
     4741 cggtgctgtc agggtcagtg tcagaacgtc cggctcttgc agcagggctt tttcgcccgg
     4801 cacaacaacg aactctttgc gtggctcgcc atcgccgggc accaactcaa aggtttttcc
     4861 ggtcagcagg gcgctgagat tagcatcgct aagggataat ttcgggttgc gtaattcgat
     4921 gcgggtattt tcacgaagca gggtaacgac gctgggatca acggtcattt ccccagtgac
     4981 tttaccacca ggatttaaat ccagtttagt cagctgtccg acttccagcc cctgatacat
     5041 taacggcgtc gagtcggcgg ttaatcctgc tccactcggc agttccagtt ttattattac
     5101 tccacgctgg ctgtgggcca gatcttcata cagaccaaag gtatcttccg cctcggcagg
     5161 tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg ccagactttc
     5221 cagtttcacc ttcgcgccac tgatactgac gttggcatca acgccggaaa cgttccagaa
     5281 acggctacct tttttcacca gatcggtaaa acgccgctcg atcagggcat caatcaccac
     5341 gccttgcttg ttgggattga tggcatagtc gtagactttt cccaccggga tcttgcggaa
     5401 atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt ggatcatcag
     5461 atcgccattg tccagccgat atttcggttg ggtatcgagc gcgacaaagt gatcctgctc
     5521 tttaccttta cccggcatca tgccgatata gttcccaccg acgagggcgt ccaacccgga
     5581 gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt cgcgcagcgc
     5641 atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat cgtcgctgag
     5701 gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg tacggcccgg
     5761 aacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt cctgataact
     5821 gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc agaaaggtga
     5881 gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt cctgactcat
     5941 gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc aaaattacgg
     6001 cagcgccaaa ataaaactca gccggtccca tagtaaaagc gaggatctga tcgcgattaa
     6061 tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt ccaatccagg
     6121 tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta aaatgaatgc
     6181 tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata ctggcgataa
     6241 acacgattcc cgcaaccgca atgttgctac ttgccagcga cataattccg gaaagaatcg
     6301 tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg ttggctggta
     6361 acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta tgacgacgac
     6421 gcaggcgtag cgggatatgg caacgcgggc agagaccacg ctgatcagga tagccggtaa
     6481 aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg cgctgcggat
     6541 aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc gttaaaatca
     6601 ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa tcctgtacct
     6661 ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac tctttaagtc
     6721 gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca aaccataaat
     6781 atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag accattgatc
     6841 ccgttatcgc atcgccctgt ttggtcattt gccagatgcc ttgcatgacg ttggcgtcaa
     6901 tacgaatgcc taacagccag atatgcaaca gcggttcgcc ccaggcaaac ggcataagca
     6961 acagcatggt gaaggccatt gccgccaggc gcgttagcga ccagtcgcgc ccgtcgcgaa
     7021 tttttgcctg acagcgcgga caataggcac tttgatgaga atttatctcg ggcaggctaa
     7081 acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc tcgccgattg
     7141 ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc atgacgtgcc
     7201 aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt gagcattaac
     7261 gcatttaatg cttattttaa taggctggca gttccaggta agacaaccaa gtgattaaat
     7321 aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc tgatggcggg
     7381 agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg agcgtctcac
     7441 tgacgttaac tgggcaggtt tttatttgct tgaggacgat acactggtac tcggaccatt
     7501 tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg gcacagcggt
     7561 tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc atattgcctg
     7621 tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc agattattgg
     7681 tgttctcgac atcgatagta ccgtcttcgc tcgctttaca gacgaggacg agcaaggctt
     7741 acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca aaaaattctt
     7801 tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg tcattataat
     7861 gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa atttcatgga
     7921 aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg aacgttttcc
     7981 ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa attggtattt ttcaggattt
     8041 ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat ccgctttacg
     8101 tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa cgcgtgtcga
     8161 tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg ctcgcaagca
     8221 gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag cgaaaaaacg
     8281 cgaagctgcc gcagctgctg gtgagaaaga agacgcaccg cgccgcgaac gcaagccacg
     8341 tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc aaaagccggt
     8401 agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc cggtttctga
     8461 catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa acgcgatgga
     8521 tgccaccgta ttagaaatca ccaaagacgg cgtccgcgtc cagctgaatt cgggtatgtc
     8581 tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca ggcatgaaca
     8641 tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag accttcgctg
     8701 tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg cagcatgcga
     8761 cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag ttcgacctcg
     8821 atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat tacagccaca
     8881 atgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag ttaggcgatg
     8941 aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg caaaagcgcc
     9001 gttttgaacg ttaccagtac gctttgtcgg tactggaaaa gccgatggat ttcaccggca
     9061 acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag gctgagttga
     9121 acgcgcagtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg acaggaaaaa
     9181 cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt cgtcgtctgg
     9241 cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg cgtgaaatcg
     9301 atccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact gaaatgagtt
     9361 tgtcactgga aggtattggc gcagtgctgc aaatggatga tgactacacc gttatcaatt
     9421 cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt gacaaaattg
     9481 tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt cttgatgatg
     9541 tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt ttacctgctg
     9601 gtaaagggac caagacccgt actgtaacgt tgacccgtga acgtattcgt ctcgaagacc
     9661 gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg ctggatattc
     9721 cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa ctggaaaaac
     9781 agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg ttgaccgaag
     9841 cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc cgcgataaca
     9901 acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa ggcccgctgg
     9961 tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg gcaatgcagg
    10021 attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc gttcagcaat
    10081 accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca gcgctgggtt
    10141 ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg caacgtaaag
    10201 gcgtaacacc agacatcatc atgccgacgg gtaatgaaga aacggaaacg ggtgagaaat
    10261 tcgaagataa cgcgctgccg tgggatagca tcgatgccgc gacttatgtg aaatcaggag
    10321 atttaactgc ctttgaaccg gagctgctga aggaacataa tgcgcgtatc gcgaaagatc
    10381 ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgaag gacaagcgca
    10441 atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat gatgcgacgc
    10501 gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg aagaagctgg
    10561 atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg gtgaatatcg
    10621 cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaaaccgct cccgtcaagt
    10681 aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag atgccgtaaa
    10741 tcagatgcta caaaatgtaa agttgtgtct ttctggtgag ttacgcacta tccagacttg
    10801 aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt taaactgagg
    10861 taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg ccgtaatggt
    10921 cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc aggggctgat
    10981 gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga tgtccaaatg
    11041 gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg aaagggaacg
    11101 ttggctggtc aatactgtag caacccaggc tcgtcaggcg gggatcgcta tgccgcaagt
    11161 ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc gtgatgcctc
    11221 tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag ccgaggcggt
    11281 aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga cgctgattca
    11341 gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc ttgccgcggg
    11401 ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc tgatctactt
    11461 tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta tcaccatgtg
    11521 gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg ttggtcgcga
    11581 gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag aagcaaccag
    11641 catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt tcatgactca
    11701 cccgccgctg gataaacgta ttgaagctct gcgtacgggt gaatacctga agtaatatga
    11761 agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccctg gctcgtggct
    11821 gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc gcagccatca
    11881 gcgagacgtg cgtaccatta tcaccaaact gatttagcat cagcgccacc agcgccgcgc
    11941 cactactctg acccagtaga cgagccgttc ctaacatgcc actggcccca ccgctacgtt
    12001 cgcgcggagc ggaggtaata atggtgtggt tattgggtga ctggaataag ccaaatccag
    12061 cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg ggcagcagaa
    12121 ccagggaaaa aagccccgca gccatgatga acaaccctaa agcacccagc aatcctgcat
    12181 gtacgcgttc aatcaaatag cctgccagcg gagccatcac catcgttgct aacggccacg
    12241 gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc aggtaaaagg
    12301 gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg caaatagaaa
    12361 gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga agagaaagct
    12421 ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca gcaattaacg
    12481 tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg aaggttaacg
    12541 cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa ccattgggtg
    12601 gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg attaaaaata
    12661 accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg gcagcagaag
    12721 agacggcaac aataaacgag tttatgccca tccctctccc cagaaaacgt tgtggataga
    12781 tcaggcggat aagtgcggta ttaacgctca tcaacgctgc accgccgaaa ccttgtatga
    12841 cacgcgcaag ggtgagcatt tgcagcgaat cagaaagggc gcagaacagt gaagacaaca
    12901 gaaaaacgac cagaccgcat ttataaatac gtcgatagcc aaacatatcg cccagaaacg
    12961 aaaacgagag cagggagatg acaatggcga tttgataggc gttcactacc cagatggaac
    13021 tggctggtgt ggcatgaagg tccgtggcga ttgttggcag ggcgacgttg gcgattgcgc
    13081 cgtcaaggac agccatcgaa ataccaatca caatggttaa tatcgcaccg tatcgctggg
    13141 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    13201 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    13261 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    13321 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    13381 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    13441 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    13501 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    13561 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    13621 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    13681 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    13741 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    13801 ctgctggcat ggcgcgatcg cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    13861 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    13921 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    13981 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    14041 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    14101 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    14161 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    14221 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttatttatt
    14281 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    14341 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    14401 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    14461 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    14521 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    14581 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    14641 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    14701 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    14761 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    14821 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    14881 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    14941 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    15001 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    15061 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    15121 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    15181 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    15241 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    15301 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    15361 tttattagga tgtttacatc agatttgtga ttaagcgtgg tattatttat tagcgaaacg
    15421 tttctctctt gatttttttg ctcactcatc aatttttctt attttaaatt tacaatccct
    15481 ttggggattg acttctcttt agggtaattg atagccgtta actgactgtt ttatgagaaa
    15541 aagtgatata actttttatt cattgcatag cagaaaatgt gatattgcac gcactatgta
    15601 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaactacgcc
    15661 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
    15721 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
    15781 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taaaagattt cgggttaaac
    15841 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
    15901 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
    15961 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
    16021 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
    16081 ctgatggcag caaagatgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
    16141 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
    16201 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
    16261 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
    16321 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
    16381 gagttgtcat gtcgttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
    16441 gctatatctg tccccaacga catcagtttg atatggcgaa agaagggtat gtcaatctgc
    16501 tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
    16561 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
    16621 tgcgggaacg gcttgatgaa aaggccacgg cggtgctgga tattggctgt ggtgaagggt
    16681 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggc ctggatgtct
    16741 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactctt tgtgtcgctt
    16801 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
    16861 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
    16921 cgccggaacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
    16981 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
    17041 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttgcct
    17101 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
    17161 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
    17221 tctggacgcc gatgccgatc agcaccagcc cgccgagaat ttccgctttt ttcccaataa
    17281 ttgagccgat aaagcgacca accatcatcc ctaatgtcga cataatcaag gttgcacaac
    17341 caatggccaa tgcggtcgcg ataatgttga cctgaaggaa agcaagacca acacccacag
    17401 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgccgac
    17461 ggtgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
    17521 cgaggaatat cagcagcaca aacgcaatcc agtgattcca ttcaaggaca aaccggctgg
    17581 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
    17641 ggccggttcg caatgcttca gaaaatttcg gtttatggag gggggcacct ttaccgattg
    17701 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
    17761 tgacaacgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
    17821 catgaacaac aagccgcgat gggttttcgc gttcatgcag gcatagcgat tgatgtgcat
    17881 atggtctcgc ctgactgaaa gtcttcagtc cgtacgtgcc acgttttgca acgagtatgt
    17941 tgacacgtac atttccggaa tctggaaatc ggctactccc caaatgaggg cgcaacctta
    18001 acatattttg agaatataaa acaactgttg gaatatatta tttcattgct ttattgataa
    18061 cgattttcat ttatgtttat atatcaatga aacgttaaaa taaataacaa ataagggaag
    18121 agataatata gcctaagcta tgtctgatta ttaaaaatac actaaacata gctttggcta
    18181 aattcataag ttattgagtt gata
//
LOCUS       DADLSB010000079        17465 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.079, whole genome shotgun sequence.
ACCESSION   DADLSB010000079 DADLSB010000000
VERSION     DADLSB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.079"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..668
                     /locus_tag="JN331_002490"
     CDS             <1..668
                     /locus_tag="JN331_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7260614.1"
                     /translation="EIRAAGSKFAIANQYRALLSDIFKTAIASGLAEEDPASATRPFR
                     TEVKRSRLLIDEYLLIRKIADVQNEWFGLCMDLALVTGQREGDLAAMRWEDIRDGRLY
                     VEQQKTGAKIRISLPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIA
                     RDTSGLKWEGDPPPFHEIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREW
                     KDI"
     gene            904..1722
                     /locus_tag="JN331_002491"
     CDS             904..1722
                     /locus_tag="JN331_002491"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAY7260615.1"
                     /translation="MTTRVIALDLDGTLLTLKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSRHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1723..2781)
                     /gene="modC"
                     /locus_tag="JN331_002492"
     CDS             complement(1723..2781)
                     /gene="modC"
                     /locus_tag="JN331_002492"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY7260616.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2784..3473)
                     /gene="modB"
                     /locus_tag="JN331_002493"
     CDS             complement(2784..3473)
                     /gene="modB"
                     /locus_tag="JN331_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY7260617.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3473..4246)
                     /gene="modA"
                     /locus_tag="JN331_002494"
     CDS             complement(3473..4246)
                     /gene="modA"
                     /locus_tag="JN331_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7260618.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4413..4562)
                     /locus_tag="JN331_002495"
     CDS             complement(4413..4562)
                     /locus_tag="JN331_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY7260619.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4691..5479
                     /gene="modE"
                     /locus_tag="JN331_002496"
     CDS             4691..5479
                     /gene="modE"
                     /locus_tag="JN331_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY7260620.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5547..7019
                     /gene="modF"
                     /locus_tag="JN331_002497"
     CDS             5547..7019
                     /gene="modF"
                     /locus_tag="JN331_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY7260621.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7280..8296
                     /gene="galE"
                     /locus_tag="JN331_002498"
     CDS             7280..8296
                     /gene="galE"
                     /locus_tag="JN331_002498"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY7260622.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8306..9352
                     /gene="galT"
                     /locus_tag="JN331_002499"
     CDS             8306..9352
                     /gene="galT"
                     /locus_tag="JN331_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY7260623.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9356..10504
                     /gene="galK"
                     /locus_tag="JN331_002500"
     CDS             9356..10504
                     /gene="galK"
                     /locus_tag="JN331_002500"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY7260624.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10498..11538
                     /gene="galM"
                     /locus_tag="JN331_002501"
     CDS             10498..11538
                     /gene="galM"
                     /locus_tag="JN331_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY7260625.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLSPGEE
                     YSSLTEYQFIAE"
     gene            11741..12493
                     /gene="gpmA"
                     /locus_tag="JN331_002502"
     CDS             11741..12493
                     /gene="gpmA"
                     /locus_tag="JN331_002502"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY7260626.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12660..13712)
                     /gene="aroG"
                     /locus_tag="JN331_002503"
     CDS             complement(12660..13712)
                     /gene="aroG"
                     /locus_tag="JN331_002503"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY7260627.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            14028..14408
                     /locus_tag="JN331_002504"
     CDS             14028..14408
                     /locus_tag="JN331_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260628.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14522..15457
                     /gene="zitB"
                     /locus_tag="JN331_002505"
     CDS             14522..15457
                     /gene="zitB"
                     /locus_tag="JN331_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY7260629.1"
                     /translation="MAHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGSLA
                     LLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVITIL
                     IVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVLG
                     DLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSL
                     DIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMD
                     HYQIEHATIQMEYQPCHGPDCHLNEGGSGHSHHHH"
     gene            complement(15454..16173)
                     /locus_tag="JN331_002506"
     CDS             complement(15454..16173)
                     /locus_tag="JN331_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY7260630.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16211..17254)
                     /gene="nadA"
                     /locus_tag="JN331_002507"
     CDS             complement(16211..17254)
                     /gene="nadA"
                     /locus_tag="JN331_002507"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY7260631.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFVAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4447 a         4550 c         4351 g         4117 t
ORIGIN      
        1 ttgaaatcag agcagctggg agtaaatttg ctattgctaa ccagtacagg gcgcttttat
       61 cagacatttt taaaacagct atagcttctg gtcttgccga agaagatcca gcttctgcaa
      121 ccaggccatt cagaactgaa gtgaagcgca gtcgactgtt aattgacgag tatctgttaa
      181 tcaggaaaat agcagacgtc cagaacgaat ggttcggcct ttgcatggat cttgcactgg
      241 taacaggaca gcgcgaagga gatctggcgg ctatgagatg ggaggatata agagatggca
      301 ggctatacgt ggaacaacaa aaaacagggg caaaaattcg catttctctc cccaccacga
      361 tttccagatt gaatttaaca cttgccgatg tactggataa cttgaaaaaa ataaacggta
      421 aaaacgaaaa gttactcgga gggaagacag ccaggacaat cgcagcacag ttcaggattg
      481 cccgagacac atccggattg aaatgggaag gtgacccacc gccatttcat gaaatccgaa
      541 gtttatccgg tcgcctgcac tcagcagaaa aaggcagtga tttcactcag gcgctccttg
      601 gacatcgttc atccagcatg acggataaat accgtgacgg acgagggagg gaatggaaag
      661 acatttagaa ttaatttcga atgatttcga aatgccactt gtaattaatt gattataaag
      721 gattaattcc taaagagcat ttgcttcatg aatgctcctt tgcatttagc aactgactgt
      781 aacagctaaa attagtcgct tttggcggtg aatgctgaat taaaacgcag aattttgtcc
      841 gatagtggta tcggtgtacc attcgcgcag acgaaaattc ttaaccttaa tctggaatac
      901 gccatgacca cacgcgtgat tgctctcgac ttagacggca ccttattgac cctgaaaaag
      961 accttgcttc catcatcgat agaagccctg gcccgcgctc gcgaagcagg ctatcaatta
     1021 atcatcgtca caggtcgcca tcacgtcgct attcatcctt tttatcaggc gctggcgctg
     1081 gatacacctg ctatttgctg taatggcacc tatttgtatg attatcatgc aaaaaccgtg
     1141 ctggaagcgg acccaatgcc cgttaataaa gccctgcaac tcattgagat gctgaatgaa
     1201 caccacattc acggtctgat gtatgtcgat gatgcaatgg tctatgagca cccgaccggg
     1261 catgtcattc gcacgtctaa ctgggcgcaa accctgccgc cggaacagcg tccgactttc
     1321 acacaagtcg cttctctggc tgaaacggcg caacaagtta acgccgtatg gaagttcgcc
     1381 ctgacgcacg atgacctgcc gcaattgcag cattttggta agcatgtcga acatgaactg
     1441 ggactggagt gtgaatggtc caggcacgat caggttgata ttgcacacgg cggcaacagc
     1501 aaaggtaaac gtttgacgaa atgggttgag gcgcaaggtt ggtcgatgga aaacgtcgtg
     1561 gcattcggcg ataactttaa tgatatcagt atgctggaag ccgctggtac aggcgtggcg
     1621 atgggcaacg ccgatgacgc ggtaaaagcg cgcgccaaca ttgtgattgg tgataacacc
     1681 accgacagca ttgcccagtt catttatagc cacctgattt aatcaggcgg ttatcgacac
     1741 acttttaatt tgcgcgtaca gccacaggcc aggtttgatc gccagttcat ccctggccca
     1801 cgggctgata cgcgcccaca gcgttttacc gccgacttcc agctccactt ccacttggcc
     1861 gttgtcgtca taactattaa caacttttgc ccgcaatacg ttacgaatgc tggtttgctg
     1921 cggcggttgt aaaaccagag aaacatcgga agcctgaatg cggatacgta gcgcagcttg
     1981 cagcggttcg cccagcttat tgacccacaa atgctgatcg cccagcgcca gcgcggtcat
     2041 cgcgtaatgc ggatgatgct caagcaccgt cactttcaga atgctacttt gttgctcttt
     2101 cggcagccac ggattcatca cgctactgcc ccacacttcc tccagcgcgc caaaggcttt
     2161 cacctgaccg ttttccagta ccatcactct gtctgccaga tggaggatct catccagcga
     2221 atggctgaca tacaacatcg gaatgttgat ttcccgtgtc agccgttgca gataaggcaa
     2281 cagttcgcgt ttacgcggaa tatccagtga cgccagcggt tcatccagca acagcaattc
     2341 cggtgctgtc agcaaagccc gaccaatcgc cacgcgctgt ttttcgcctc cggacaggct
     2401 gcctggtaaa cggtcaagca acggttcaat gcctaaaagc gccaccagct tatcgaactg
     2461 atcgaccata cttttcgaca tgccgtagcg cagattgcca cgcactttgt aatgcgggaa
     2521 cagccgcgca tcctgaaaaa catagccaac gcgacgcttt tccggcgtca ggcagatacc
     2581 tttttcggca tcatttagta cccgcccatt gaggacaatc cgcccttttt gcgggcgcgt
     2641 cagtccactg atggcgttaa tcagcgaagt ttttccggca ccggagacgc caaagatagc
     2701 agtgatgcca ttggcgggca gcgtttcatt aatggtcagg caatggttgc ccaacgtctg
     2761 ggaaaaattc agctccagca tgattagcgc cccgcccgtt cacggctgat tctggccagc
     2821 cattctgaaa tcaacaggga gatcatcgcc agcgcaatag aaataatgca cagtctcgcc
     2881 gcgccacttt ccccgccggg ggtctggatc agggtataca tggcagaagg aatggttcgc
     2941 gtttcgctag gaatgttcga cacaaaggtg atggttgcac caaactcacc gagagaacga
     3001 gcaaaagcca gtaccgtacc aacaataatt cccggtaagg tcagcggtaa cgtgatagta
     3061 aagaaaacgc gccagcgccc ggcacctagt gttcttgcgg cctgttccag tttgacatca
     3121 accccttcca gcgccagacg aattgcccgc accatcagcg gaaacgacat gacggcggca
     3181 gcgagaaccg cgccgcgcca gctaaaggcg aaggtaatac caaaccagtc atacagacgt
     3241 tcaccgataa atccgcgccg tcccatcgaa actaataata agtaaccgac gaccacgggc
     3301 ggtaacacca gcggtagatg cagtacgctg tcgagcagag ctttgcccgg aaacgtgcaa
     3361 cgcaccagta accaggcaaa aaagatccca aacggcaggc taaacagcac agccagggaa
     3421 gaaactttca ggcttaataa aactgcctgc cattctggat cggtcagtat cattacttgg
     3481 ttgtaaatcc gtaacgttta aagatttccg ctgcctgcgg tcccttcaga taatcataaa
     3541 atgctttcac tgtcgcattg ttatgccctt ccacaacagc aaccggatat tccacttttt
     3601 tatgtgaatc ttccgggaag gtggcaacca cttttacccc tttgctggca actgcgtcag
     3661 aaccgtagac aatgcccaga ggcgcttcgt tacgttcgac cagcgccagc gccccacgaa
     3721 catcttccgc tggggccagt ttcggagaga gcgtatccca tgcgcccagt ttttgcagtg
     3781 cttcttttgc ataaatgcca gcgggaacat gttccggatc gccaaccgcc aggcgaccgc
     3841 cattcagcag cgaagtccag ttagttttgc tgtcgatggt gaaatctttc tgctcgctgg
     3901 ctttcggtgc tacaacgacc aggctattgc cgagcagtgt ctgacgcgta gcagtatcga
     3961 tcgctttttt atcaaccgca taatccatcc atttctgatc ggcagaaata aacagatccg
     4021 caggggcacc cgcttcaatc tgacgggcga gagtagatga cgaagcgaaa gaagaaacca
     4081 catccacgcc tttctctttt ttatactgcg tagcaatgtc ctgcattgcg ttagtcagtg
     4141 atgcggcggc gaacaccgtg attttccctt catctgccag tgcattgcca gcaactgcga
     4201 aagagagtgc cgccccggca aacaagttca accatttacg agccattggt aaccccttaa
     4261 tgtaacgttg tgtaggcgac aatataacga ctaactcgga attttcccag cagttattgc
     4321 taacctttgt gaggtagata agaaaaaata tcggcaggaa aagcaggaag ttgagaaaaa
     4381 gaaaatgccc gactaagcgg gcattcaggg aatcaatgat tttgtccggg ctggtctttt
     4441 ttaccaacac cagaaaagat gttgaatact tcaccaagac cgtaaatcag acccaggatg
     4501 atggccatca cgacaggtac catgattacg gcgaatacca gactttttaa taactctaac
     4561 atggtcaact ccagatatag tcatgagact attctaaccg ctaagcacag aaaagcactc
     4621 cccttttgtg cggtcagctt tgcgtggcgt tcgttttccg tcacaataag acttttgcca
     4681 ggacattgtt atgcaggccg aaatccttct cacccttaag ctccaacaaa aattattcgc
     4741 cgacccgcgc cgcatttcgc tactaaaaca cattgcgctt tccggttcca ttagccaggg
     4801 agcgaaagat gccggtatca gctataaaag cgcctgggat gccattaacg agatgaatca
     4861 gttaagtgag catattctgg tcgagcgcgc aacaggcggt aaaggtggcg gaggcgcagt
     4921 actgacccgc tatggtcagc gactgattca gctctatgac ttactggcgc aaatccagca
     4981 aaaagccttt gatgtgttaa gtgacgatga cgccctgccg ctgaacagcc tgctggccgc
     5041 gatctcacgt ttttcactgc aaaccagcgc ccgtaaccag tggttcggta ccatcaccgc
     5101 ccgcgatcat gatgacgttc aacagcatgt tgatgtctta ctggctgacg gaaaaacacg
     5161 cctgaaagtc gcaattaccg cacaaagcgg cgcacgtctg gggctggatg aaggcaaaga
     5221 agtgttgata ttgctaaaag cgccgtgggt aggcattact caggacgaag cggtcgcaca
     5281 aaacgccgac aaccaattac cgggtattat tagtcatatt gagcgcggcg cagagcagtg
     5341 cgaagtatta atggcgctac ccgacgggca aacactgtgc gccacagtgc cggtaaatga
     5401 agcgacttct cttcagcaag ggcagaatgt cacggcctac tttaatgccg acagcgtgat
     5461 tatcgccacg ctgtgctaag cgtgttgaca atttgttatg aaacacgtat ccctgtcagt
     5521 aatcgctgca caaaatggga tataaaatgt catcgttgca aattttgcaa ggcacgtttc
     5581 gtcttagcga cacaaaaacg ctgcaattgc ctcagctaac gttaaacgcg ggtgatagtt
     5641 gggcgtttgt cggttcgaat ggaagtggaa agtcggcgct ggcccgcgcg ctggcggggg
     5701 aacttccgct tttgaaaggt gaacggcaaa gccagttttc ccacatcact cgtctctcct
     5761 tcgagcaatt gcaaaagctc gtcagcgacg aatggcaacg gaataacacc gatatgctcg
     5821 gccctagcga agatgacacc ggacgcacta cggctgagat tattcaggat gaagtaaagg
     5881 atgcaccgcg ttgcatgcaa ctggcgcagc agttcggtat taccgccctc ctcgaccgac
     5941 gctttaaata cctttccact ggcgagacgc gaaaaaccct gctgtgtcag gcgctgatgt
     6001 cggagcctga cttgttgatt cttgatgagc cgttcgatgg cctggatgtt gcctcacgtc
     6061 agcagctggc tgagcgactc gcctcgttac atcagtccgg tattactttg gtactggtgc
     6121 tcaatcgctt cgatgagatc ccggaatttg tccagtttgc tggcgtgctg gcggattgta
     6181 cgttagcgga aactggcgct aaagaggaac tgctccagca agcactcgtc gcgcaacttg
     6241 cgcatagtga acagcttgaa ggtgtgcaac tgccggagcc ggatgaacct tcagcacgtc
     6301 acgccttacc cgccaacgaa tcgcgcattg tgctgaacaa tggcgtggtt tcttataatg
     6361 atcgccccat tcttaataac cttagctggc aggtgaatcc aggcgaacac tggcaaattg
     6421 tcgggccaaa tggcgcggga aaatcgacgt tattaagcct gattactggc gatcatccgc
     6481 aaggttacag caacgatttg acgcttttcg gccgacgtcg cggcagcagc gaaaccatct
     6541 gggacatcaa aaagcatatc ggttacgtca gcagtagttt gcatctggat taccgggtca
     6601 gcactaccgt gcgtaatgtg attctttctg gctattttga ttcgattggc atttatcagg
     6661 ccgtttcgga tcgccagcaa aaactggtgc agcagtggct ggatattctc ggcattgata
     6721 aacacacggc tgacgctccg ttccatagtc tttcctgggg acagcagcgt ctggcgctga
     6781 tcgtccgcgc actggtgaaa catccgacgt tgcttattct cgatgaacca ctacaggggc
     6841 ttgatccgct caatcgccaa cttatccgcc gttttgttga tgtgctgatt agcgaaggtg
     6901 aaacgcaatt gttgtttgtt tcgcaccacg ctgaagatgc gcctgcctgt attacccatc
     6961 gccttgagtt cgtgccggac ggtggactct atcgctatgt gctgacaaaa atatattgag
     7021 tcggtagtgc tgaccttgcc ggaggcggcc taagcgccct ctccggccaa cggttcgacg
     7081 catgcaggca tgaaaccgct tcttttttca gataaaaagc gcaatcattc ataaaccctc
     7141 tgttttataa tcacttaatc gcgcataaaa aacggctaaa ttcttgtgta aacgattcca
     7201 ctaatttatt ccatgtcaca cttttcgcat ctttgttatg ctatggttat ttcataccat
     7261 aagcctaatg gagcgaatta tgagagttct ggttaccggt ggtagcggtt acattggaag
     7321 tcatacctgc gtgcaattac tgcaaaacgg tcatgatgtc atcattcttg ataacctctg
     7381 taacagtaag cgcagcgtac tgcctgttat cgagcgttta ggcggcaaac atccaacgtt
     7441 tgttgaaggc gatattcgta acgaagcgtt gatgaccgag atcctgcacg atcacgctat
     7501 cgacaccgtg atccacttcg ccgggctgaa agccgtcgcc gaatcggtac aaaaaccgct
     7561 ggaatattac gacaacaatg tcaacggcac tctgcgcctg attagcgcca tgcgcgccgc
     7621 taacgtcaaa aactttattt ttagctcctc cgccaccgtt tatggcgatc agccccaaat
     7681 tccatacgtt gaaagcttcc cgaccggcac accgcaaagc ccttatggca aaagcaaatt
     7741 gatggtggaa cagatcctca ccgacctgca aaaagcccag ccggactgga gcattgccct
     7801 gctgcgctac ttcaacccgg ttggcgcgca tccgtcgggc gatatgggcg aagatccgca
     7861 aggcattccg aataacctga tgccatacat cgcccaggtt gctgtaggcc gtcgcgactc
     7921 gctggcaatt tttggtaacg attatccgac cgaagatggt actggcgtac gcgattacat
     7981 ccacgtaatg gatctggcgg acggtcacgt cgtggcgatg gaaaaactgg cgaacaagcc
     8041 aggcgtacac atctacaacc tcggtgctgg cgtaggcagc agcgtgctgg acgtggttaa
     8101 tgccttcagc aaagcctgcg gcaaaccggt taactatcat tttgcaccgc gtcgcgaagg
     8161 cgaccttccg gcctactggg cggacgccag caaagccgac cgtgaactga actggcgcgt
     8221 aacgcgcaca ctcgatgaaa tggcgcagga cacctggcac tggcagtcac gccatccaca
     8281 gggatatccc gattaaggaa cgaccatgac gcaatttaat cccgttgatc atccacatcg
     8341 ccgctacaac ccgctcaccg ggcaatggat tctggtttca ccgcaccgcg ctaagcgccc
     8401 ctggcagggg gcgcaggaaa cgccagccaa acaggtgtta cctgcgcacg atccagattg
     8461 cttcctctgc gcaggtaatg tgcgggtgac aggcgataaa aaccccgatt acaccgggac
     8521 ttacgttttc actaatgact ttgcggcctt gatgtctgac acgccagatg cgccagaaag
     8581 caacgatcct ttgatgcgtt gccagagcgc gcgcggtacc agccgggtga tctgcttttc
     8641 accggatcac agtaaaacgt tgccagaact gagcgttgcg gcattgacgg aaatcgtcaa
     8701 aacctggcag gagcaaaccg cagagctggg aaaaacgtac ccgtgggtgc aggtctttga
     8761 aaacaaaggt gcggcgatgg gctgctctaa cccgcatccg cacggacaga tttgggcaaa
     8821 tagcttcctg cctaacgaag ctgagcgcga agaccgcctg caaaaagaat attttgccga
     8881 acagaaatca ccaatgctgg tggattatgt tcagcgcgag ctggcagacg gtagccgtac
     8941 cgttgtcgaa accgaacact ggttagccgt tgtgccttac tgggctgcct ggccgttcga
     9001 aacgctactg ctgcccaaag cccacgtttt gcggatcacc gatttgaccg acgcccagcg
     9061 cagcgatttg gcgctggcgt tgaaaaagct gaccagtcgt tatgacaacc tcttccagtg
     9121 ctccttcccc tactctatgg gctggcacgg cgcaccattt aatggcgaag agaatcaaca
     9181 ctggcagctg cacgcgcact tttatccgcc tctgctgcgc tccgccaccg tacgtaaatt
     9241 tatggttggt tatgaaatgc tggcagagac ccagcgagac ctgaccgcag aacaggcagc
     9301 agagcgtttg cgcgcagtca gcgatatcca ttttcgcgaa tccggagtgt aagaaatgag
     9361 tctgaaagaa aaaacacaat ctctgtttgc caacgcattt ggctaccctg ccactcacac
     9421 cattcaggcg cctggccgcg tgaatttgat tggtgaacac accgactaca acgacggttt
     9481 cgttctgccc tgcgcgattg attatcaaac cgtgatcagc tgtgcaccac gcgatgaccg
     9541 taaagttcgc gtgatggcag ccgattatga aaatcagctc gacgagtttt ccctcaatgc
     9601 gcccattgtc gcgcatgaaa actatcaatg ggcgaactac gttcgtggcg tggtgaaaca
     9661 tctgcaactg cgtaacaaca gcttcggcgg tgtggacatg gtgatcagcg gcaatgtgcc
     9721 gcagggtgcc gggttaagtt cttccgcttc actggaagtc gcggtcggaa ccgtattgca
     9781 gcagctttat catctgccgc tggacggcgc acaaatcgcg cttaacggtc aggaagcaga
     9841 aaaccagttt gttggctgta actgcgggat catggatcag ctaatttccg cactcggcaa
     9901 gaaagatcat gccttgctga ttgactgccg ctcactgggg actaaagcag tttccatgcc
     9961 gaaaggtgtg gctgtcgtca tcatcaacag taacttcaaa cgtaccctgg ttggcagcga
    10021 atacaacacc cgtcgtgaac agtgcgaaac cggtgcgcgt ttcttccagc agccagctct
    10081 gcgcgatgtc accattgaag agttcaacgc tgttgcacat gagctggacc caatcgtggc
    10141 gaaacgcgtg cgtcatatcc tgactgaaaa cgcccgcacc gttgaagctg ccagcgcgct
    10201 ggagcaaggc gacctgaaac gtatgggcga gttgatggcg gagtctcatg cctctatgcg
    10261 cgatgatttc gaaatcaccg tgccgcaaat tgacactctg gtagaaatcg tcaaagctgt
    10321 gattggcgac aaaggtggcg tacgcatgac cggcggcgga tttggcggct gtatcgtcgc
    10381 gctgatcccg gaagagctgg tgcctgccgt acagcaagct gtcgctgaac aatatgaagc
    10441 aaaaacaggt attaaagaga ctttttacgt ttgtaaacca tcacaaggag caggacagtg
    10501 ctgaacgaaa ctcccgcact ggcacccgat ggtcagccgt accgactgtt aactttgcgt
    10561 aacaacgcag ggatggtagt cacgctgatg gactggggtg cgactttact ttccgcccgt
    10621 attccgcttt ccgatggcag cgtccgcgag gcgctgctcg gctgtgccag tccggaatgc
    10681 tatcaggatc aggctgcgtt tctgggggcc tctattggtc gttatgccaa ccgcatcgcc
    10741 aatagtcgtt atacctttga cggtgaaacc gtgacgcttt cgccaagcca gggcgttaac
    10801 cagctgcacg gcggcccgga agggttcgac aaacgtcgct ggcagattgt gaaccagaac
    10861 gatcgtcagg tgctgtttgc cctgagttca gatgatggcg atcagggctt cccgggtaat
    10921 ctcggcgcga cggtgcaata tcgtctgacc gacgataacc gtatctccat tacttatcgc
    10981 gccacagttg ataaaccttg cccggtgaat atgactaatc acgtctattt caatcttgac
    11041 ggcgagcagt ctgacgtgcg caatcacaag ttgcagattc tggcggacga atatcttccg
    11101 gttgatgaag gcggcattcc gcacgacggc ttgaaatctg tcgccggaac gtcttttgat
    11161 ttccgcagcg ccaaaatcat cgccagtgag tttcttgccg acgacgatca gcgcaaagtg
    11221 aaaggttacg atcacgcatt cttgttacag gccaaaggcg atggcaagaa agtggcggcg
    11281 catgtctggt cagcagatga aaaattgcag ctgaaggtct acaccaccgc tccggctctt
    11341 caattctact ccggcaactt cctcggcggc acaccgtcgc ggggaaccga accttacgcc
    11401 gactggcaag ggctggcgtt ggaaagcgag tttctgccgg acagcccgaa ccaccctgaa
    11461 tggccgcaac cggactgctt cctgagtcct ggcgaagagt attccagcct gacggaatat
    11521 cagtttattg ctgagtaatg ttaagccctc gtcatgaggg ctttatcttc atattgttca
    11581 aatcaccagc aaacaccgac atatttgcaa ctcaatattc acaacaacct tacactgcgc
    11641 cactattttc gctatggtta tgcgtaagca ttgctgttgc ttcgtcacgg caatataatg
    11701 agaattatta tcattaaaag atgatttgag gagtaagtat atggctgtaa ctaagctggt
    11761 tctggttcgt catggcgaaa gtcagtggaa caaagaaaac cgtttcaccg gttggtacga
    11821 cgtggatctg tctgagaaag gcgtaagcga agcaaaagca gcaggtaagc tgctgaaaga
    11881 ggaaggttac agctttgact ttgcttacac ttctgtgctg aaacgcgcta tccataccct
    11941 gtggaatgtg ctggacgaac tggatcaggc atggctgccc gttgagaaat cctggaaact
    12001 gaacgaacgt cactacggtg cgttgcaggg tctgaacaaa gcggaaactg ctgaaaagta
    12061 tggcgacgag caggtgaaac agtggcgtcg tggttttgca gtgactccgc cggaactgac
    12121 taaagatgat gagcgttatc cgggtcacga tccgcgttac gcgaaactga gcgagaaaga
    12181 attgccgctg acggaaagcc tggcgctgac cattgaccgc gtgatccctt actggaatga
    12241 aaccattctg ccgcgtatga agagcggtga gcgcgtaatc atcgccgcac acggtaactc
    12301 cctgcgtgcg ctggtgaaat atcttgataa catgagcgaa gaagagattc ttgagctgaa
    12361 tatcccgact ggcgtgccgc tggtgtatga gttcgacgag aatttcaaac cgctgaaacg
    12421 ctattatctg ggtaatgctg acgagatcgc ggcgaaagca gcagccgttg cgaaccaggg
    12481 taaagcgaag taaacgtcat tcgtttaaaa aatgagaaag ccgactgcaa gtgagtcggc
    12541 ttttttgttt gtcgttgcta acaatggagc acattgcctg atgcgacgct gcgcgtctta
    12601 tcaggcctgt ggtgattcat cggatacgcc actctgacgg cgcatccgac aattaaacct
    12661 tacccgcgac gcgcttttac tgcattcgcc agttgacgta acagagcatc ggtatcttcc
    12721 cagccaatgc aggcatcggt gatgctctta ccgtaggcca gcggctcccc gctatcgagg
    12781 ctctgattgc cttccaccag atggctttcc accatcacgc caataatggc cttttcgcca
    12841 ccggcaatct gctggcaaac gtcagcacaa acatccatct gctttttgaa ttgtttggac
    12901 gaattagcat ggctgaaatc gatcatcacc tgagctggca gacctgcttt gttcagccct
    12961 tctttcactt cagcaacgtg cttcgcgctg tagttaggct ctttaccgcc gcgcagaatg
    13021 atatggcaat cgccgttacc actggtattc acaatcgccg aatgccccca tttagttacg
    13081 gacaggaagc agtgcggcgc accggcggca ttaatggcat cgatagccac tttaatcgta
    13141 ccgtctgtgc catttttaaa acctaccgga caagaaagac cagacgccag ttcgcggtgc
    13201 acctgcgatt ccgtggtacg tgcaccaatt gcgccccagc tcatcaggtc agcgagatac
    13261 tgaggagtga tcatatcgag aaactcaccc gccgctggca gaccgctgtc gttaatatca
    13321 agcagcaatt tacgggctat acgcagaccg tcgttgatct ggaagctgtt atccatatgc
    13381 ggatcgttaa tcaacccttt ccagcccacc gtggtacgcg gcttttcaaa atagacgcgc
    13441 attacgattt ccagctcatc tttcagctct tcacgcagcg ccagcaagcg agtggcatac
    13501 tctttagccg cgacaggatc atgaattgag cacgggccaa tcaccaccaa caggcgatca
    13561 tcattacctt tcaggatctt atggatcgct tttcgggcat gggcaaccgt attcgcggca
    13621 ttttcagttg cggggaattt ttccagcaat gcgacaggag gaagtaactc tttgatttct
    13681 ttgatgcgta aatcgtcgtt ctgataattc atgtctgttc cagtgttgcc atacttatct
    13741 tagtgaatgc aatacttcca atctatatct tccgtcagaa tgtgtaaacg gggttttaca
    13801 ctatgaacgg attaatcctg gaattagcaa gaaaaacgcc agattgtcgc gaaaaacgag
    13861 atctatccta caatttctaa ctgtaactcc tttgtttgtt aattatttca agattctctg
    13921 ctgcgtttca taaactggct gaaatcttaa accaatgcct tatattcacc tgcaaatgca
    13981 ctgttggaag aggttatccg acataacgac cataacagga gcatcctatg aaaatgacaa
    14041 aactggccac attatttctg actgccactc taagccttgc cagcggtgcc gcactggctg
    14101 ccgatagcgg agcgcaaact aataacggcc aggcaaacgc cgcagctgat gcgggccagg
    14161 tagcccctga cgcccgtgaa aatgtcgcgc caaataacgt cgacaataac ggggtaaata
    14221 ccggttctgg cggcacaatg ctgcattcgg atggttcttc aatgaacaat gacggaatga
    14281 ccaaagatga agagcacaag aacaccatgt gcaaagatgg tcgatgtccg gatatcaata
    14341 aaaaggtaca aaccggtgat ggcatcaata atgatgtcga taccaaaacc gacggtacca
    14401 cgcagtaacc ctgcaaaata gccaataatg ggagagcgga gtctctccct tttaattttt
    14461 cacttctaaa agcagtatga tgtcataaag ttaagtgaga ataattaagg gatgactcgt
    14521 catggcgcac tcacacacgt cctcacacct gccagaagat aataatgctc gtcgcttgtt
    14581 gtatgctttc ggcgtgactg ccgggtttat gctggtagaa gtcgttggtg gttttctttc
    14641 tggttctctg gcattgctgg ccgatgcggg tcatatgttg accgatactg ccgccctgct
    14701 tttcgcccta ctcgccgtgc aattttcacg tcgtcctccc accattcgcc acactttcgg
    14761 ttggctaaga ctgaccacgc tcgcggcgtt tgtgaatgcc atcgccctcg tggtaattac
    14821 cattttgatt gtctgggagg cgatagaacg cttccgcacg ccgcgtccgg tcgagggagg
    14881 catgatgatg gcaattgccg tggctgggtt gctggcaaat atactttctt tctggttact
    14941 tcatcacggc agcgaagaga aaaacctcaa cgtacgagca gcggcactgc atgtgctcgg
    15001 ggacctgctg ggttcggttg gggctattat tgccgcgttg attattatct ggactggctg
    15061 gacacctgct gaccccattc tctcgatact ggtgtcgctt ctggttctgc gtagcgcgtg
    15121 gcgactgttg aaagatagcg tgaatgaatt acttgaaggt gcaccggtat cgctggatat
    15181 cgctgaactg aagcgtcgta tgtgccggga aatcccggaa gtccgcaatg tgcaccatgt
    15241 acatgtatgg atggtaggcg agaagccggt gatgacgttg catgtgcagg tgatcccgcc
    15301 acacgatcac gatgccttgt tggatcagat ccaacactac ctgatggatc actatcagat
    15361 tgagcacgcc accattcaga tggaatatca accttgtcat gggccagact gccacctgaa
    15421 tgaagggggg tctggccatt cacatcatca ccattaatgg gacagcgcgc gtgagcctct
    15481 ttcacgtgcg ctgttgatcc acatccggct gccgttgagc gcaataaagg tcaggatgat
    15541 gtactccaga gacatggcgt aaacgccctg aagtgcaaaa ataacgacgc taatcacgtt
    15601 aataatcacc cacaacagcc agttttccac atacttacgc gtcatcagaa tcattgccac
    15661 gatagataac accatcatgc atgaatccca gaacgggaaa gcgtccggtt gcagttcagg
    15721 catcacaacc tgtaatccta atgcttgcat gatcatgact gccacgcggg tcaaaaatgc
    15781 aaacaccgga ttgataaata ccgtcatcag accaatcgaa acaacgcaaa ccgccaacca
    15841 gctgagtgcc ttcggcaatg gcaaccagcg aattttcaac tccgcctcgt tctgactggt
    15901 ttgtcgcgac cacgcatacc agccgtaaat attcgcggca aagaaaaaca cctgtaatag
    15961 caggctggca tacagctgaa tctgaaagaa aataatgcca aacaaggtaa cgttgatcag
    16021 gccaaagaag tagttgctga tcttctccag actggcaagg ccaatacaca gcaacccggc
    16081 gatcgtgcct accgcttcaa tccatgagag atcataaccg cccgccccta ttggtatatg
    16141 taccaggata ttctgcacac taaaaaaatc catctttccc ccaaagcgta acgttacgcc
    16201 ttattattcg ttatccacgt agtgtagccg caaaatccag catacgattg agcggcacca
    16261 gcgccctctc tcgcagcctc tcatcgacat gaacctcgtg attacttcct tcctgttcta
    16321 atgcctctgc gatagcctga aggccattca tggccatcca cggacaatgc gcgcagctgc
    16381 ggcaggttgc gccctcaccc gcggttggtg cttccagtaa ctctttatct ggcaccgcct
    16441 gctgcatttt gtagaaaata ccccgatcgg ttgccacaat aagcctctga tgaggcaatg
    16501 cttttgcagc agcgatcagt tgactggtgg aaccgaccgc atccgccata tcgacaatag
    16561 cttgtggtga ttctggatgc accagtatgg cagcatccgg gtattcttct tgcaagcggg
    16621 ttaacgcctg agtcttaaat tcatcatgca caatgcaggc accctgccag cataaaatgt
    16681 ctccacccgt ctgtttttgc acgtaacgcc ccagatgttt gtcgggtgcc cagatgattt
    16741 tttcacccaa actatcaaga tgatcaataa gttcgacggc aatgcttgaa gttaccaccc
    16801 aatctgcgcg cgcttttacc gcagcagaag tgttggcgta gacgacgaca gtacgatcgg
    16861 gatgggcatc gcaaaatgcg ttaaattctt caacagggca gccgagatcc agtgaacact
    16921 cagcctgaag tgtcggcatc agaattgttt tttccggact gagaattttg gcggtttctc
    16981 ccataaatct caccccagcg actaacaaag tagaagcggg atgctttgca acgaagcgcg
    17041 ccatttccag agaatcagaa atacagccac cagtttcttc tgccagttgt tgaatttcgg
    17101 gatcggtata gtagtgggca accatcaccg cattacgttc ttttagcaga cgttttatct
    17161 tctcgcggta atacgctttt tcatcaatgc ttaacggcgt cggcttcggg gggaaaggat
    17221 aaatcgccgt gtctggatca aacattacgc tcatcttacc atctcgtttt acaggcttaa
    17281 cgttaaaacc gacattagcg taacattcgc tgttatcggt gtaatttgtt ttatatgcta
    17341 aacaagatag tcgaatggtg gggaaaagtc actcgaatta cgcctaattc caccgacgta
    17401 gagttgcttg cacagtaagc tctgacgatg tcatcaggtt tacaaagtga aattgaggat
    17461 gcctg
//
LOCUS       DADLSB010000080        17306 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.080, whole genome shotgun sequence.
ACCESSION   DADLSB010000080 DADLSB010000000
VERSION     DADLSB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.080"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JN331_002508"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JN331_002508"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY7260632.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQVIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JN331_002509"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JN331_002509"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY7260633.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRALRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JN331_002510"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JN331_002510"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY7260634.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEASLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JN331_002511"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JN331_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY7260635.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JN331_002512"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JN331_002512"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY7260636.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JN331_002513"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JN331_002513"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7260637.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JN331_002514"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JN331_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY7260638.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JN331_002515"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JN331_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7260639.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVL
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JN331_002516"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JN331_002516"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY7260640.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JN331_002517"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JN331_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY7260641.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /locus_tag="JN331_002518"
     CDS             complement(14847..15542)
                     /locus_tag="JN331_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7260642.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JN331_002519"
     CDS             complement(15665..16702)
                     /locus_tag="JN331_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7260643.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..>17306
                     /locus_tag="JN331_002520"
     CDS             17035..>17306
                     /locus_tag="JN331_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="YhhY; regulated by the fur regulator; unknown
                     function; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7260644.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRW"
BASE COUNT         4248 a         4740 c         4310 g         4008 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgac ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaaga gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggcg cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcactgtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag actggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcactgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg acttaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgca tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagt cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aagcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caagttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccaa
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac agacgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgcccg cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatttgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acgcgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgaaacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaa
//
LOCUS       DADLSB010000081        17212 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.081, whole genome shotgun sequence.
ACCESSION   DADLSB010000081 DADLSB010000000
VERSION     DADLSB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.081"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(2..94)
                     /locus_tag="JN331_002521"
     tRNA            complement(2..94)
                     /locus_tag="JN331_002521"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="JN331_002522"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="JN331_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY7260645.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JN331_002523"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JN331_002523"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY7260646.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKQEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLGRTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVTEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JN331_002524"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JN331_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY7260647.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JN331_002525"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JN331_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY7260648.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JN331_002526"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JN331_002526"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY7260649.1"
                     /translation="MTDSELMQLSEQVGQTLKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6113..7198)
                     /gene="mltB"
                     /locus_tag="JN331_002527"
     CDS             complement(6113..7198)
                     /gene="mltB"
                     /locus_tag="JN331_002527"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY7260650.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7454..8017
                     /gene="srlA"
                     /locus_tag="JN331_002528"
                     /gene_synonym="gutA"
     CDS             7454..8017
                     /gene="srlA"
                     /locus_tag="JN331_002528"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY7260651.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="JN331_002529"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="JN331_002529"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY7260652.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGIKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="JN331_002530"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="JN331_002530"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY7260653.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="JN331_002531"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="JN331_002531"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY7260654.1"
                     /translation="MNQVAVAIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="JN331_002532"
                     /pseudo
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="JN331_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="JN331_002533"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="JN331_002533"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY7260655.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="JN331_002534"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="JN331_002534"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY7260656.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="JN331_002535"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="JN331_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY7260657.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEMVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="JN331_002536"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="JN331_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY7260658.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLNLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELTSEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="JN331_002537"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="JN331_002537"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY7260659.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..>17212)
                     /locus_tag="JN331_002538"
     CDS             complement(16795..>17212)
                     /locus_tag="JN331_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="carbamoyltransferase; involved in the formation of
                     the cyanate group of hydrogenase; catalyzes the formation
                     of carbamoyladenylate from carbamoylphosphate and then
                     transfers the carbamoyl group to HypE; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY7260660.1"
                     /translation="SYEGEAACALEALAASCDGVTHPVTMPRVDNQLDLATFWQQWLN
                     WLAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLAD
                     FTLLFPQSLPAGDGGLSLGQGVIAAARWLAGEVQNG"
BASE COUNT         4067 a         4735 c         4380 g         4030 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggtt accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt cccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tcctgtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttaccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggtg tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaagcaatt atactgtata
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcactgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgtctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc tcttgtaaat agctactgta
     6121 ctcgcgccag cgccacggct tgtcctaact gccagaccgc cattgcgtaa tgggtgctgt
     6181 ggttgtaacg ggtgatggtg tagaagttcg gcagaccgta ccagtactgg tagccggtgc
     6241 caacatccag acgcagcagg ctggcttgtt gatggttgcc cagcggctgc tgtggcgtta
     6301 aacctgcggc ggcaagctgc gaaatgctgt acttagtttt gaaaccattt ggcaagcctg
     6361 gagcctgacc gtttgccatt atcgcgacct gatcgccttt cacccagccg tgcgctttga
     6421 aatagttcgc cacgctaccg atcgcatcaa ccggatccca caggttgata tgcccgtcgc
     6481 cgctgaaatc taccgcatat tgtttgtaag acgacggcat aaactgtccg tagcccatcg
     6541 ccccggcaaa ggaacctttc agattgagcg gatcgtcctg ctcgtcgcgc gccatcagca
     6601 ggaaggtttc cagctcgcca gagaaatact ccgcgcggcg tgggtagtta aatgacagcg
     6661 ttgccagcgc atcgaggatg cgagttttcc ccatcacgcg cccccagcgg gtttcaacgc
     6721 cgataatccc gacgataatt tccggcggta cgccatacac ctgccacgcg cgattcaacg
     6781 catcttcata ctgattccag aaaaccacac cgttctgcac gttgtccggc gtaataaatt
     6841 ttttgcgata acgcagccat gcgccgttcg gacctgatgg cggtttcacc gatgtggttg
     6901 gtgcctggtt atccatcagc cgcagtaccg aatccagacg cttcgcctgg gagagaattt
     6961 cctgcaactg ctgacgatcg aaaccgtgtt tgttcaccat tttgtcgatg aactgctggg
     7021 cattcgggtt attagcgaaa tcgccgccca tctgcatcac attgtgctgc ggctcaagca
     7081 ggaagccgcc agacggcgtt ccggtggtcg tttcagtctc agtaggtttt ggcttgctgc
     7141 tacaggcggc aagcaacaca aaaaggggaa gcaatgttac ataacgacgc ttgaacatga
     7201 ggggtccatt taacagattc aaccaggggc aagtatggta aagcatcacg ccccgcacaa
     7261 ggaagcggta gtcactgccc gatacggact ttacataact caactcattc ccctcgctat
     7321 acttttattc aaactttcaa attaaaatat ttatctttca ttttgcgatc aaaataacac
     7381 ttttaaacct ttcaatctga ttagattagg ttgccgtttg gtaataaaac aataaatcct
     7441 gaaggagaga acaatgatag aaaccattac tcatggtgca gagtggttta tcgggctgtt
     7501 ccaaaagggc ggagaggtgt ttaccgggat ggtgaccggc attcttccgc tgttgattag
     7561 cctgctggtt atcatgaacg cactgattaa ttttatcggt cagcatcgta ttgaacgttt
     7621 tgctcaacgt tgcgccggta accctgtttc ccgttaccta ctgttaccgt gcattggcac
     7681 gtttgtcttt tgcaatccga tgaccttaag cctgggtcgc tttatgccgg aaaagtacaa
     7741 acccagctac tacgcggcgg cctcttatag ctgccactca atgaatggcc tcttccccca
     7801 tatcaaccct ggcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcataa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctggcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcctgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgcca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcc gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacgcggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc accatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcggtggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcaacctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactga catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccgg tgccagccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac tt
//
LOCUS       DADLSB010000082        16948 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.082, whole genome shotgun sequence.
ACCESSION   DADLSB010000082 DADLSB010000000
VERSION     DADLSB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.082"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(316..1512)
                     /gene="hemY"
                     /locus_tag="JN331_002539"
     CDS             complement(316..1512)
                     /gene="hemY"
                     /locus_tag="JN331_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY7260661.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1515..2738)
                     /gene="hemX"
                     /locus_tag="JN331_002540"
     CDS             complement(1515..2738)
                     /gene="hemX"
                     /locus_tag="JN331_002540"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7260662.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQGDTPAAAPQGE"
     gene            complement(2760..3500)
                     /gene="hemD"
                     /locus_tag="JN331_002541"
     CDS             complement(2760..3500)
                     /gene="hemD"
                     /locus_tag="JN331_002541"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY7260663.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3497..4438)
                     /gene="hemC"
                     /locus_tag="JN331_002542"
     CDS             complement(3497..4438)
                     /gene="hemC"
                     /locus_tag="JN331_002542"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY7260664.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVVLLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4825..7371
                     /gene="cyaA"
                     /locus_tag="JN331_002543"
     CDS             4825..7371
                     /gene="cyaA"
                     /locus_tag="JN331_002543"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY7260665.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7411..7731)
                     /gene="cyaY"
                     /locus_tag="JN331_002544"
     CDS             complement(7411..7731)
                     /gene="cyaY"
                     /locus_tag="JN331_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY7260666.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7779..9092)
                     /locus_tag="JN331_002545"
     CDS             complement(7779..9092)
                     /locus_tag="JN331_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY7260667.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYPNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9089..10554)
                     /locus_tag="JN331_002546"
                     /pseudo
     CDS             complement(9089..10554)
                     /locus_tag="JN331_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            10743..10946
                     /locus_tag="JN331_002547"
     CDS             10743..10946
                     /locus_tag="JN331_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7260668.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            10983..11807
                     /gene="dapF"
                     /locus_tag="JN331_002548"
     CDS             10983..11807
                     /gene="dapF"
                     /locus_tag="JN331_002548"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY7260669.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11804..12511
                     /locus_tag="JN331_002549"
     CDS             11804..12511
                     /locus_tag="JN331_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY7260670.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLVRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12508..13404
                     /gene="xerC"
                     /locus_tag="JN331_002550"
     CDS             12508..13404
                     /gene="xerC"
                     /locus_tag="JN331_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY7260671.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13404..14120
                     /gene="yigB"
                     /locus_tag="JN331_002551"
     CDS             13404..14120
                     /gene="yigB"
                     /locus_tag="JN331_002551"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY7260672.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14204..16366
                     /gene="uvrD"
                     /locus_tag="JN331_002552"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14204..16366
                     /gene="uvrD"
                     /locus_tag="JN331_002552"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY7260673.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16513..16944)
                     /locus_tag="JN331_002553"
     CDS             complement(16513..16944)
                     /locus_tag="JN331_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260674.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3894 a         4418 c         4522 g         4114 t
ORIGIN      
        1 attatctcat aacaagtgtt aagggatgtt atttcccgat tctctgtggc ataataaacg
       61 agtagatgct cattccatct cttatgttcg ccttagtgcc tcataaactc cggaatgacg
      121 cagagccgtt tacggtgctt atcgtccact gacagatgtc gcttatgcct catcagacac
      181 catggacaca acgttgagtg aagcacccac ttgttgtcat acagacctgt tttaacgcct
      241 gctccgtaat aagagcaggc gtttttttat gcatcagaaa ggtcccggag gtgctttcct
      301 ccgggtgaga aggaactact gtggcgggtt attctgcaac gttaacatca aaccgtcgcg
      361 acgcatcgct gcagcttctt ccggcttgtg cagtctgtcc agcgcgtcgg caagccatgc
      421 gtaatcgtag gcgtccggac gttgtttcag cgccgcgcgg aaggcgagcg atgcttcctg
      481 ccattcaccg tgcttcatca gtgactgacc caatgtgctc cacaacagcg ggcgatcacc
      541 gacgtttttg atttgctggc gtagcacttt ttccagctgt tccgggttat tggttttcag
      601 acgcgggatc ggcagcagta ggcgatcgtc atactggcgt ttcaggccgt cgatgataat
      661 ttgctgggca gtatcatgat cgtcacattc aataagatgt tccgccattg ccacctgcaa
      721 cgctacctga tgacgcgttt tccggctttg gtttttccac cagttacgca aaccttcact
      781 accgttatcg gcacgcgcct gatccatcag gccaatccat gcctgttgtt ccagcattgc
      841 acgatgttct tcatcaccaa catgggcttt cgccattgat gggatgatat ccagcagaga
      901 actccatgcc cctgtgcgga tatacgcctg ttccgccaga cgtaatactt caggatggcg
      961 tggcgtaact tccagcagct tatccacgcc gtggcgtgca gcatggtttt cattacgggc
     1021 cagttgcaga cgtacacggg tgatttctac cggaatggtg tcattgccgg ccagctccgc
     1081 tgcgcgttcc agatgttggt tggcgcgtgc ttcatcacca cgttgttgcg cggcttcggc
     1141 agccagtaga tagttcacca ccggttgttc agcgtgatcg gcatttttcg ccatcagctt
     1201 ttcaacttgc tgataatcac cttccgccag tttcagcagc gcctgttcgg tctgcttacg
     1261 cgcacggcga cgcttgcgtc cgacaaacca tccacgggtg tgcgcgccag tgcggaagat
     1321 ccgccgcagt agccactcaa tggcaaacag cactaccatc gccagaatca atatgatcgc
     1381 caggcccgtg acgctggttt cgatattgta gttgtcggtc tggatcagca cataaccctg
     1441 atgaccggca atcatcgggc caaccactat ccccgcaatc agcaaaacaa agagtaataa
     1501 cacttttagc atggttattc tccttgcggc gcggctgccg gagtatcacc ttgcggtgca
     1561 ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg tttagcttcc
     1621 gttgtccccg ctgccggttg tgccagcagg ttacgcacgc gagtctgcat cagtttttcc
     1681 agcatcgcct ggctttgcag ggtttccgga aggtccatcg agatattttg ctggcttaac
     1741 tggtccacct cgtcgaggaa cgctttggtg gtggcatcat cagtatcgta gtaagcacgt
     1801 acccaggtgg agacgttctc cagcgcctgg cgataagtct cttcctgatg acgcggtaca
     1861 gcttgtgctg cgaccagcag gcgagagcga atattttcgc gcagatagat atcctgattt
     1921 ggcgctaaca gcggtacggc ggtgtcatca cgacggcgaa tcgtaatgaa gttgtccata
     1981 aagttctgcc agcttttttg cagattgata cgccattcgc tgatggaact ggaaagctct
     2041 tcaccgtctg aatccatcgg cgaaccatcg ctgtcattat cggccagacg caggttatct
     2101 acctgatttg aaagctgatt aagcttaagg atgatgccgt cataatccac ctgcgatact
     2161 gcagaaaggc tggcgatatc atcggtaatt gcccgacgaa cggtaatcag actcgggtca
     2221 ttcatatccg ccaggctggc gtctgcactt ttcagcaacg ctgcagaggt cgtgacgtcc
     2281 tgatcgctcc acagcttccg tccggcgagt ttcaccagaa aatcggcctg agccagcagc
     2341 caggttttag catcgctgcc ggaaatggtg gcgatctttt gttggacttc atccaactgt
     2401 tttgccagcg tttcttgctg acgattcgcc tgcttaagct gcgcagcttg ttgcttaata
     2461 atgccttcca gctcggcttt ttggctctcc tgggcttttt gcaatgccgt cagttggtta
     2521 gccagggcat cgctggtggc ggtctgattg acggcctgtt gtttacccca gccatacaaa
     2581 ccgacacccg ccgccagagc aatagcgata gccaccgcgc tgagaatcaa tgcagtattg
     2641 ttcttactct ttttttctgt tgcgacaggt tgtgacgtgg tgtccacggc ctccctagtc
     2701 tcttcaacca cggcggaggt tttttcttgt tccgtcatta tggcttcctg ttatgagagt
     2761 tattgtaatg cccgtaaaag cgcatcgttg tcagcgttat cggcgacctt aatgtcttgc
     2821 cagcccagtt cccgggcgag tttcgccaaa cgctcactga cgaccaatag tcgacagtgt
     2881 agtaaccagt gctcacgata ccattgtggg atcagcgacc agagttgctg caacatttca
     2941 ccgctggtaa caacgaccat cgtcacctcg cgggattgcc agcgcatcgc ttcttctgca
     3001 ccatcgtaat ggattgcgca tcgttgataa cattcacaaa aagtgacctc agcaccgcgc
     3061 accgtcaggg tatccccaat tagctcacga ccgccattgc cacgtaatat cagcgcacgt
     3121 ttgcccgcaa tattttgtaa ttcaggtaat tgtagcaaga cttcgctgat ttcccgatcc
     3181 tgtgggtaga gaatcttctg tccgcttacg gtatgtagcg ccagtgcggt ggtgcgtcca
     3241 atggcgaaat aagtaggtag ttggggccat ttacgatctt gctgatgcag ctgtgattgg
     3301 gcaaaagcaa ccgcgtgttg cgagagggca aacaacagat cgctctcccc cagcgctgcc
     3361 agttgatcag caagttgcgg taattgtcga cccggagaaa actcaatcag cggaaaatgc
     3421 caggccacct gccccagtgt gcgcagacgg ctcactaact cttctccagc gggagacggg
     3481 cgggtgacca ggatactcat gccggggcct ctccgttata gacttcagcg aggatctcgc
     3541 gcgcgccgtt attcagtagc tcttctgcca gcgaaatccc catttgttcg gcatcttgcg
     3601 gcgcaccgcg gcgttcaccg cgaataatct gcgaaccgtc cggcgcgccg accagcgcac
     3661 gtagccagat ttcgccatca ataagctcgg cgtagctacc aattggcacc tgacatccgc
     3721 cttcgagacg ggtattcatg gcgcgttctg cggtaacgcg cagtgcagtt tcgtggtgat
     3781 tcagcgcggc aagcagctcg cgagtgcgtg tatcatcaag gcggcattca ataccgactg
     3841 caccttgtcc taccgccgga agagaaatct cgggtggtaa cgcggcgcga atgcgggact
     3901 ccagacctaa acgttttagt ccggctacgg caagaatgat ggcatcgtat tcgccgttat
     3961 ctagtttgct caggcgagtg ccgacgttgc cgcgcaggga gcggataatc agatccgggc
     4021 ggcgttcagc cagttggcac tggcgacgta aactggacgt cccgacgata ctgcctgctg
     4081 gtaacgcatc cagactgtca tagttattgg acacaaaggc atcgcgagga tcttcacgct
     4141 cgcaaatagt gaccagcccc agaccttgcg ggaattcaac cggcacatct ttcattgaat
     4201 gtacggcgat atcagcgcga ttctccagga gcacgacttc cagctctttt acaaataagc
     4261 cttttccgcc tactttcgcc agcggcgtat caagaatcac atcgccgcgc gtcaccatcg
     4321 gtaccagttc aacgaccagg cccggatggc tcgccatcaa cttgtctttg acatagtgtg
     4381 cctgccagag tgcaagtggg ctttggcgtg tggcaattct taaaacattg tctaacatgc
     4441 ttgttaccgt cattatcatc cgtggtccat cctaacatcc ttgccagagt gatgtcagtg
     4501 ttgtggtgaa acgtagacgc ctgcgcaaac cgtaaaatga ggtctggcag tggatcctga
     4561 caggcgtttc acgccgtcgt aataaggaat ttacagagaa taaacggtgc tacacttgta
     4621 tgtagcgcat ctttctttac ggtcaatcag caaggtgtta aattgatcac gttttagacc
     4681 attttttcgt cgtgaaacta aaaaaaccag gcgcgaaaag tggtaacggt tacctttgac
     4741 atacgaaata tcccgaatgc cgcgtgttac cgttgatgtt ggcggaatca cagtcatgac
     4801 gggtagcaaa tcaggcgata cgtcttgtac ctctatattg agactctgaa acagagactg
     4861 gatgccataa atcaattgcg tgtggatcgc gcgcttgctg ctatggggcc tgcattccaa
     4921 caggtctaca gtctactgcc gacattgttg cactatcacc atccgctaat gccgggttac
     4981 cttgatggta acgttcccaa aggcatttgc ctttacacgc ctgatgaaac tcaacgccac
     5041 tacctgaacg agcttgaact gtatcgtgga atgtcagtac aggatccacc gaaaggtgag
     5101 cttccaatta ctggtgtata caccatgggc agcacctcgt ccgtagggca aagttgttcc
     5161 tctgacctgg atatctgggt ctgtcatcaa tcctggctcg atagcgaaga gcgccagtta
     5221 ctacaacgta aatgcagcct gctggaaagc tgggccgcct cgctgggtgt ggaagtcagc
     5281 ttcttcctga ttgatgaaaa ccgcttccgt cataatgaaa gcggcagcct ggggggcgaa
     5341 gattgtggct ccacccagca tatactgctg cttgacgaat tttatcgtac tgccgtgcgt
     5401 ctcgccggta agcgtattct gtggaatatg gtgccgtgcg acgaagaaga gcattacgac
     5461 gactatgtga tgacgcttta cgcgcagggc gtgctgacgc caaatgaatg gctggatctc
     5521 ggtggcttaa gctcgctttc tgctgaagag tactttggtg ccagcctttg gcagctctac
     5581 aagagtatcg attccccata caaagcggtg ctgaaaacac tgctgctgga agcctattcc
     5641 tgggaatacc cgaacccacg tctgctggcg aaagatatca aacagcgttt gcacgacggc
     5701 gagattgtat cgtttggcct cgatccatac tgcatgatgc tggagcgtgt tactgaatac
     5761 ctgacggcga ttgaagattt cacccgtctg gatttagtac gtcgctgctt ctatttaaaa
     5821 gtgtgcgaaa aactcagccg tgaacgcgcc tgtgtaggct ggcgtcgcgc agtgttgagc
     5881 cagttagtga gcgagtgggg ttgggacgaa gctcgtctgg caatgctcga taaccgtgct
     5941 aactggaaga ttgatcaggt gcgtgaggcg cacaacgagc tgctcgacgc gatgatgcag
     6001 agctaccgta atctgatccg ctttgcgcgt cgcaataacc ttagcgtctc cgccagtccg
     6061 caggatatcg gcgtactgac gcgtaaactg tatgccgcgt ttgaagcatt gccaggtaaa
     6121 gtgacgctgg taaacccgca gatttcaccc gatctctcgg aaccgaatct gacctttatt
     6181 tatgtgccgc cgggccgggc taaccgttca ggttggtatc tgtataaccg cgcgccaaat
     6241 attgagtcga tcatcagcca tcagccgctg gaatataacc gttacctgaa taaactggtg
     6301 gcgtgggcat ggtttaacgg cctgctgacc tcgcgcaccc gtttgtatat taaaggtaac
     6361 ggcattgtcg atttgcctaa gttgcaggag atggtcgccg acgtgtcgca ccatttcccg
     6421 ctgcgcttac ctgcaccgac accgaaggcg ctctacagcc cgtgtgagat tcgccatctg
     6481 gcgattatcg ttaacctgga atatgacccg acagctgcgt tccgcaatca ggtggtgcat
     6541 tttgatttcc gtaagctgga tgtcttcagc tttggcgaga atcaaaattg cctggtaggt
     6601 agcgttgacc tgctgtaccg caactcgtgg aacgaagtgc gtacgctgca cttcaacggc
     6661 gagcaatcga tgatcgaagc cctgaaaact attctcggca aaatgcatca ggacgccgca
     6721 ccgccagata gcgtggaagt cttctgttat agccagcatc tgcgcggctt aatccgtact
     6781 cgcgtgcagc aactggtttc tgagtgtatt gaactgcgcc tttccagcac ccgccaggaa
     6841 accgggcgtt tcaaggcgct gcgcgtttct ggtcaaacct ggggcttgtt cttcgaacgc
     6901 ctgaatgtat cggtacagaa gctggagaac gccatcgagt tttatggcgc tatttcgcat
     6961 aacaaactgc acggcttgtc agtgcaggtt gaaaccaatc acgtcaaatt accggcggtg
     7021 gtggacggct ttgccagcga agggatcatc cagttctttt tcgaagaaac gcaagacgag
     7081 aatggcttta atatctacat tctcgacgaa agcaaccggg ttgaggtgta tcaccactgc
     7141 gaaggcagca aagaggagct ggtacgtgac gtcagtcgct tctactcgtc atcgcacgac
     7201 cgcttcacct acggctcaag cttcatcaac ttcaacctgc cgcagttcta tcagattgtg
     7261 aaggttgatg gtcgcgaaca ggtgattccg ttccgaacaa aatcgattgg caacctgccg
     7321 cccgccaatc aggatcacga tacgccgcta ttacagcagt atttttcgtg atgagcgtgc
     7381 cggaaagcga ggcttatccg gcatgcaatc ttagcggaaa ctgactgttt cacccgcctg
     7441 ttgcgtcgcc gcctgttcca gcaaatccca gaaggtttcg ccgctgcgat cgcaaatcca
     7501 ttcatcgcct ttcaggtcaa aatggtagcc gccctgtttg gttgccagcc acacctggtg
     7561 cagcggctcc tggcggttaa taatgatttt gctgccgttt tcaaaggtaa tggtcagtac
     7621 gccgccgttg atttcgcagt cgatatcgct gtcgccatcc ctgtcgtcca gacgctcttc
     7681 aatggtcagc cacagttgat cagccaggcg atgaaattca ctgtcgttca ttgttgtatc
     7741 ctgtttttaa gtgatggcgg cagtatagcg gcatggggtc agggcttcaa agtttgcacc
     7801 tctgctgctg cgttccggca tgattcatcc gtcaccggaa taatgatgtc tctgttttga
     7861 acttcacgta cgttctcctc tctgacacga gttttcgccg ggcaggggga gggcgtaatc
     7921 cttacctctg tatcggtcca tgtataaaca ttgcgatcgt cataagcgaa gcgatcgttt
     7981 ttagttagcg gaccgttaac tggaataatt tgcaagctct ttgaccgtaa ttggcggata
     8041 aagtaagcat gctgtttgcc gctattaaat gagcgatcaa tgaccaggtc tggcgtatcc
     8101 agcgggatta tctcaagctg gtcttcacca aatgtcgata attttgttac atacagccga
     8161 tctttatatt gcgcaatatt gccagtaatc agatggaatt gttcaggttc aatacctggt
     8221 aggtctatcc acttgctcca ctgacggtcg ggcgtgagct gtttacgcca gcgaacgcga
     8281 ctttccccta cttcaaaaga gacgtcatca tctacgggta tcgctttgcc tgataattga
     8341 ccaaagggat aacgatgcag acccttattg tcgcgaaaaa ccagcagtga atgaggtatc
     8401 cagcgaatta tctcaaatga gtctgcattc gccgtaaggt gaacgccatt taaataaata
     8461 ttctgcccac tgcggataag cagatcattg ggatatgaat ttctgtttac actcataaga
     8521 cgtggattaa taccccacca tcttttttct aatacagtaa catcactgct gctcccctga
     8581 cgtcgaccgg acaagtagag atttttacta tccaccagcg tggtagagtt accttcgata
     8641 gcttttagtg tcgccagatc aacacgtgac gcgccgctat tactttcggt gcgctgtccg
     8701 tcaaaataga ggctatattt atcgaccgcg aagcggccca tagcctgaaa gctggcaata
     8761 tcgacagtgg gtgttcccgg tggattgcta acgattttgc cacgccagag aataacgcgg
     8821 ccgtctgtca cccagtggta ttcctcattt tctatgctta tttcatcgga aggtatataa
     8881 cccgttttcc cagagatatt tttcacaagg cgcaaatcgg ccctgctcat ctccggaaac
     8941 tgggcaatga ttttcttatc cccccaataa cctttaccgt aagacataac accgtgatca
     9001 ttgtaatagt cgaaccctat ggcctgtaca gcaggcgtca gaaatgtgtg tcccaacagg
     9061 atgagtgcaa ctgtgattat ttgacccttc attttgtcgc ctcattgcgc ggggcaggaa
     9121 taaattcgcc gtcatcagta atttttccgt ccagatatcc cgttttgtaa tttacgccat
     9181 agccatatcc cgaatgttgt tccttccaga ttaagcgaac ttgtttcgga tcagcggtgc
     9241 ggtatcgcca gagctgcgcg ccatcaaagc tgtaaatgta gcggttatcg cgggcatacc
     9301 atctgcgcaa aaagggatgg tcattaagat gggcttcgcg ttcatccgga gaacggttat
     9361 ccagtaagat cagcggtccg tcagattcaa acacgtgtaa accggaatgg tatggccact
     9421 cttcggcacg cgtaagtaaa tagccgtggc gtttcccggc gttgaaacgt ttgttgataa
     9481 taagattggg atcatcaagt ttcacgatct ccagcttacg gtcaccagac tctgtttcga
     9541 tgatggtata aagcgagtcc tgatactggg cgacagcatc actgatgggt tgaaactgct
     9601 ccggatcgag tccgggtaat tcttcctgtt tacaagcagg tcctttacgc cagaacactt
     9661 tcttttccag cagagaaaaa tccagacagt gctttgttaa atcttcatcc cacgccagat
     9721 tttccttgtc gaggacttta cgctgcagcc cgtttttatc acgccaggtc aagaatgagc
     9781 cgggcatcca gcgcacgacg gaaaaggtgt ccgggtcggc atcaagctgt tcgccgttga
     9841 gcaggatttt ggtccgtgta cgagccaggg tatcccaggg gccaaatggt acggcaatgc
     9901 aggggttaaa cgtggtggaa aactttcctc gctgatccta ggacttctgc gccagcacac
     9961 ggaaactctc ggggtcagcg aggcgatgcc cattgatata caagaaatta gcgtcacgta
    10021 atatcagccc cagtaagtca ggatcccagg gaggccggaa ttccacctga tgcagcgttt
    10081 tgatatccaa gctatttcca ccaccattgt catcggtgcg cttgccttca aaatagagac
    10141 tgtgtttatc tgcggcaaaa tcaccccatg ccttgaacga ggcgacatcg acgggaggtg
    10201 tgccgggagg gttttgcaca acttcacccg cgtagaggat attgcgacca tcgctatgcc
    10261 atgaataatg aggataccag gcctgtgcgc cctcatagag gtagtcggtt gtcaaatcag
    10321 tcagaggcgg ttctttatcc cattcgtcta gtgttgtcgg gtctattaat aaattgggta
    10381 accgacgcaa actcttgtaa ttcacgtcag gtagagaaag atttgtttct ttataagggc
    10441 gcaggtacac cacgcctttt ccctgtttct ggtaaatatt atgagccgac atccccatgc
    10501 cgacgataaa aacaatgaac aagccaacaa acaaaccaca ttgcgatagt gcataaagcc
    10561 atcctggcgc gaggtgttga tcacgaaact gccagcagaa cataaatcct cacgagtaag
    10621 cgttatactc gcagcatttc ctcacttttc agacttcata aagagtcgct aaacgcttgc
    10681 ttttacgcct tctcctgcga tgatagaaag cagaaagcga tgaactttac aggcaatcca
    10741 taatgaaaaa cgtgtttaag gcactcactg tattacttac tctcttcagc ctgacgggct
    10801 gcggtctgaa aggtccgctc tatttcccgc ctgcagataa aaacgcaccg ccgccgacca
    10861 aaccggtaga gacgcaaacg caatccacgg tgccggataa aaacgatcgc gccactggcg
    10921 atggtccatc ccaggtgaat tactaaaagt cagtttctgt acccgcgtga ttggagtaaa
    10981 tgatgcagtt ctcgaaaatg catggccttg gcaacgattt tatggtcgtc gacgcggtaa
    11041 cgcagaatgt ctttttttca ccggagctga ttcgtcgcct ggcggatcgg cacctggggg
    11101 tagggtttga ccagctgctg gtggttgagc cgccgtatga tcctgaactg gattttcact
    11161 atcgcatttt caatgctgat ggcagtgaag tggcgcagtg cggtaacggt gcgcgctgct
    11221 ttgcccgttt tgtgcgtctg aaaggactga ccaataagcg tgatatccgc gtcagcaccg
    11281 ccaacgggcg gatggttctg accgtcaccg atgatgatct ggtccgcgta aatatgggcg
    11341 aacccaactt cgaaccttcc gccgtgccgt ttcgcgctaa caaagcggaa aagacctata
    11401 ttatgcgcgc cgccgagcag acaatcttat gcggcgtggt gtcgatggga aatccgcatt
    11461 gcgtgattca ggtcgatgat gtcgataccg cggcggtaga aacgcttggt cctgttctgg
    11521 aaagtcacga gcgttttccg gagcgcgcca atatcggttt tatgcaagtg gttaagcgcg
    11581 agcatattcg tttacgcgtt tatgagcgtg gggcaggaga aacccaggcc tgcggcagcg
    11641 gcgcgtgtgc ggcggtggcg gtagggattc agcaaggttt gctggacgaa gaagtacgcg
    11701 tggaactccc cggcggtcgt cttgatatcg cctggaaagg tccgggtcac ccgttatata
    11761 tgactggccc ggcggtacat gtctacgacg gatttattca tctatgaagc aaccagggga
    11821 agaactgcag gaaacactca cggagcttga tgaccgggcg gttgtcgatt atctgattaa
    11881 aaatcctgag ttttttatcc gcaatgcgcg cgcagtggaa gcgatacgtg tgccgcatcc
    11941 ggtacgcggc accgtttcgt tggtcgagtg gcacatggcc cgcgcacgta atcacattca
    12001 tgttctggaa gagaacatgg cgttgttgat ggagcaggct atcgccaacg aagggctgtt
    12061 ttatcgcctg ctctacctgc agcgcagtct caccgccgcc agcagtctcg acgatatgct
    12121 ggtgcgcttt caccgctggg cgcgcgatct cggcctggct ggtgcgagtc tgcgcctgtt
    12181 tccagatcgc tggcgcttag gtgcgccgtc gaaccacact catctggcat taagccgtca
    12241 gtctttcgaa ccgctgcgta ttcagcgttt ggggcaggaa cagcactatc ttgggccgct
    12301 taacggaccg gagctgctgg tggtgctacc ggaagcgaaa gcggtgggat cggtggcgat
    12361 gtcgatgctg ggaagcgatg ctgatttggg tgttgtgctg tttactagtc gcgatgccag
    12421 tcactatcaa caagggcagg gaacgcagtt acttcatgaa attgcgctga tgttgccgga
    12481 gcttctggag cgttggattg aacgcgtatg accgatttac acaccgatgt agaacgctac
    12541 ctacgttatc tgagcgtgga gcgtcagctt agcccgataa ccctacttaa ctaccagcgt
    12601 cagcttgagg cgatcatcaa ttttgccagc gaaaacggcc tgcaaagctg gcaacaatgc
    12661 gatgcagcga tggtacgcaa ttttgctgta cgcagtcgcc gtaaagggct gggagcagca
    12721 agtctggcgt tacggctttc tgcgctacgt agcttttttg actggctggt cagccagaac
    12781 gaactcaaag ctaacccggc gaaaggagtt tcggcaccga aagcgccgcg tcatctgccg
    12841 aaaaatatcg acgtcgacga tatgaatcgg ctgctggata ttgatatcaa cgatcccctc
    12901 gctgtacgcg accgtgcaat gctggaagtg atgtacggcg cgggtctgcg tctttccgag
    12961 ctggtggggc tggatatcaa acacctcgac ctggagtccg gcgaagtgtg ggtgatgggg
    13021 aaaggcagca aagagcgccg cctgccgatt ggtcgcaacg ctgtggcgtg gattgagcac
    13081 tggcttgatt tgcgcgacct gtttggtagc gaagacgacg cgctttttct gtcgaaactg
    13141 ggcaagcgta tctccgcgcg taatgtgcag aaacgctttg ccgaatgggg cataaaacaa
    13201 gggctgaata atcacgttca cccacataaa ttacgtcact cgttcgctac gcatatgctg
    13261 gagtcgagcg gcgatcttcg tggtgtgcag gagctactgg gtcatgccaa cctctccacc
    13321 acgcaaatct atactcatct tgattttcaa caccttgcct cggtgtacga tgcggcgcat
    13381 ccacgcgcca aacgggggaa ataatgcgtt tttaccggcc tttggggcgc atctcggcgc
    13441 tcacctttga cctggatgat accctttacg ataaccgtcc ggtgattttg cgcaccgagc
    13501 gagaggcgct tacctttgtg caaaattatc atccggcgct gcgcagcttc cagaatgaag
    13561 atctgcaacg cctgcgccag gcggtacggg aagcggaacc cgagatttat cacgacgtga
    13621 cgcgttggcg ttttcgttcg attgaacaag cgatgctcga cgccgggctg agtgccgaag
    13681 aagccagtgc aggcgcacac gcagcaatga ttaactttgc caaatggcgc agccgaatcg
    13741 acgtcccgca gcaaactcac gacaccttaa aacagctggc gaagaaatgg ccgctggtgg
    13801 cgatcaccaa cggtaacgcc cagccggaac tgtttggttt gggggattat tttgagtttg
    13861 tgctgcgcgc tggcccgcac gggcgctcaa aaccgttcag cgatatgtac tttttggctg
    13921 cggaaaaact caacgtgccg atcggcgaga tcttacatgt tggggacgat ctcaccactg
    13981 acgtgggtgg ggcaattcgc agcggaatgc aggcttgttg gatcagaccg gaaaatggcg
    14041 atctgatgca aacctgggac agccgtttac tgccacatct ggaaatttcc cggttggcat
    14101 ctctgacctc gctgatataa tcagcaaatc tgtatatata cccagctttt tggcggaggg
    14161 cgttgcgctt ctccgcccaa cctattttta cgcggcggtg ccaatggacg tttcttacct
    14221 gctcgacagc cttaatgaca aacagcgcga agcggtggcc gcgccacgca gcaaccttct
    14281 ggtgctggcg ggcgcgggca gtggtaagac gcgcgtactg gtgcatcgta tcgcctggct
    14341 gatgagcgtg gaaaactgct cgccatactc gattatggct gtgacgttta ccaacaaagc
    14401 ggcggcggag atgcgtcatc gtatcggaca actgatgggc accagccagg gcggcatgtg
    14461 ggtaggcacc ttccacgggc tggcgcaccg cctgctgcgt gcgcaccata tggacgccaa
    14521 tctgccgcag gatttccaga tcctcgatag cgaagaccag ctacgcctgc ttaagcgtct
    14581 gatcaaagcc atgaacctcg acgagaagca gtggccgccg cgccaggcaa tgtggtacat
    14641 caacagccag aaagatgaag gcctgcgtcc gcatcatatt caaagctacg gtaatccggt
    14701 ggagcagacc tggcagaagg tgtatcaggc gtatcaggaa gcgtgcgatc gcgcgggcct
    14761 ggtggacttc gccgagctgc tgctgcgcgc tcacgaactg tggcttaaca agccgcatat
    14821 cctacaacac taccgcgaac gttttaccaa tatcctggtg gacgaattcc aggataccaa
    14881 caacattcag tacgcgtgga tccgcctgct ggcgggcgac accggcaaag tgatgatcgt
    14941 cggtgatgac gaccagtcaa tctacggctg gcgcggggcg caggtggaga atattcagcg
    15001 tttccttaat gatttccccg gtgccgaaac tattcgtctg gagcaaaact accgctctac
    15061 cagcaatatt ctgagcgccg ctaacgccct gattgaaaac aataacgggc gtctgggtaa
    15121 aaaactgtgg accgatggcg cggacggtga gcctatttcc ctctattgcg cttttaacga
    15181 actcgatgaa gcgcgttttg tggttaaccg catcaaaacc tggcaggaca acggcggggc
    15241 gcttgccgag tgcgccattc tctaccgcag taacgcccag tcgcgtgtgc tggaagaagc
    15301 actgttgcag gcgagtatgc cgtaccgtat ttacggcggg atgcgcttct tcgaacgcca
    15361 ggaaatcaaa gatgcgctct cgtatctgcg cctgattgcc aaccgtaacg acgacgcggc
    15421 ctttgagcgt gtggtgaata cgccaacgcg gggtattggt gaccggacgc tggacgtggt
    15481 acgtcagaca tcgcgcgatc gccagttaac actctggcag gcatgtcgtg aactgttgca
    15541 ggaaaaagcc ctcgccggac gtgctgccag cgccttacag cggtttatgg aactgatcga
    15601 cgccttagcg caggaaactg ccgatatgcc gctgcatgta cagactgacc gggtaattaa
    15661 agactccggc ctgcgtacca tgtacgagca ggagaagggc gaaaaaggtc agacgcgtat
    15721 cgaaaactta gaggaactgg tgacggcaac gcgccagttc agctacaacg aagaagacga
    15781 agatttaatg ccgctgcagg cgttcctatc ccatgcggcg ctggaagcag gcgaagggca
    15841 ggcggatacc tggcaggatg cggtacaact gatgaccatg cactcggcga aaggtctgga
    15901 gttcccgcag gtgtttatcg tcggtatgga agagggcatg ttcccaagcc agatgtcgct
    15961 ggatgaaggc gggcgtctgg aagaagaacg ccgtctggcc tacgttggcg taacccgcgc
    16021 gatgcagaaa ctgacgctga cctacgcgga aactcgccgt ctgtatggca aagaggttta
    16081 ccatcgcccg tcgcgcttta ttggcgagtt gccggaagag tgtgtggaag aggtgcgcct
    16141 gcgcgccacg gtaagccgcc cggtcagcca tcagcggatg ggtacgccga tggtcgagaa
    16201 cgacagcggc tacaagctcg gccagcgtgt acgccacgct aagtttggtg aaggcaccat
    16261 cgtcaatatg gaaggcagcg gtgaacatag ccggttgcag gtggcattcc agggccaggg
    16321 aatcaaatgg ctggtggcag cttacgcccg gctggagtcg gtgtaacgtt gccggatgcg
    16381 gtgctgcgca ccttatttgg cctaaaaaat cattcagatt caataaattg caacgtcatg
    16441 taggccggat aaggcgttta cgccgcatcc ggcatcagca gcatatttag tatctgacac
    16501 aaagctatca ttctaacctt tccgctcgac ggaaatcatg gtgacaaaag gatagcgttg
    16561 ccacgggatt gccccgcctt tcatatacat atgtgaaatc gtgccatcaa gataaagcag
    16621 ctgctcaacg ttcagtttcg ctttggcata acaggcaaaa tcataaaaat ttgtcgcctg
    16681 ctggctcaac aaaaacacgg cattcccatg tttattaatc ccaacaccgt tacgaatttt
    16741 acgtgaggcg acgttgggat gaatacgcgg attaattaca ccgttttcca tcaacattgg
    16801 tcctgactgc accgcaaact gaatatcttt actggcttta aaagcatcca gacgaacgat
    16861 gccgacttta tctcccgcga cataaaacac gccgccagga cggataaaga aattcccttc
    16921 acctgaagcg agatttaacg ccaccttc
//
LOCUS       DADLSB010000083        16935 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.083, whole genome shotgun sequence.
ACCESSION   DADLSB010000083 DADLSB010000000
VERSION     DADLSB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.083"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            190..1323
                     /gene="fepE"
                     /locus_tag="JN331_002554"
     CDS             190..1323
                     /gene="fepE"
                     /locus_tag="JN331_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY7260675.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(1320..2135)
                     /gene="fepC"
                     /locus_tag="JN331_002555"
     CDS             complement(1320..2135)
                     /gene="fepC"
                     /locus_tag="JN331_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7260676.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(2132..3124)
                     /gene="fepG"
                     /locus_tag="JN331_002556"
     CDS             complement(2132..3124)
                     /gene="fepG"
                     /locus_tag="JN331_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAY7260677.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3121..4125)
                     /gene="fepD"
                     /locus_tag="JN331_002557"
     CDS             complement(3121..4125)
                     /gene="fepD"
                     /locus_tag="JN331_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAY7260678.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4236..5486
                     /gene="entS"
                     /locus_tag="JN331_002558"
     CDS             4236..5486
                     /gene="entS"
                     /locus_tag="JN331_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAY7260679.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            complement(5490..6446)
                     /gene="fepB"
                     /locus_tag="JN331_002559"
     CDS             complement(5490..6446)
                     /gene="fepB"
                     /locus_tag="JN331_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7260680.1"
                     /translation="MRLAPLHRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGAATPNNRVADDQGFLRQWSKVAKERKLQQLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            6737..7912
                     /gene="entC"
                     /locus_tag="JN331_002560"
                     /pseudo
     CDS             6737..7912
                     /gene="entC"
                     /locus_tag="JN331_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            7922..9532
                     /gene="entE"
                     /locus_tag="JN331_002561"
     CDS             7922..9532
                     /gene="entE"
                     /locus_tag="JN331_002561"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAY7260681.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGHLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            9546..10403
                     /gene="entB"
                     /locus_tag="JN331_002562"
     CDS             9546..10403
                     /gene="entB"
                     /locus_tag="JN331_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAY7260682.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            10403..11149
                     /gene="dhbA"
                     /locus_tag="JN331_002563"
     CDS             10403..11149
                     /gene="dhbA"
                     /locus_tag="JN331_002563"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAY7260683.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            11152..11565
                     /gene="entH"
                     /locus_tag="JN331_002564"
     CDS             11152..11565
                     /gene="entH"
                     /locus_tag="JN331_002564"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAY7260684.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            11746..13851
                     /gene="cstA"
                     /locus_tag="JN331_002565"
                     /pseudo
     CDS             11746..13851
                     /gene="cstA"
                     /locus_tag="JN331_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
     gene            14033..14230
                     /locus_tag="JN331_002566"
     CDS             14033..14230
                     /locus_tag="JN331_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7260685.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(14240..15328)
                     /gene="hcxA"
                     /locus_tag="JN331_002567"
     CDS             complement(14240..15328)
                     /gene="hcxA"
                     /locus_tag="JN331_002567"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAY7260686.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            15437..16597
                     /gene="ybdL"
                     /locus_tag="JN331_002568"
     CDS             15437..16597
                     /gene="ybdL"
                     /locus_tag="JN331_002568"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HAY7260687.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            complement(16598..>16935)
                     /locus_tag="JN331_002569"
     CDS             complement(16598..>16935)
                     /locus_tag="JN331_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260688.1"
                     /translation="MEIVDGFHHHEIGKGSSSLKLRLKGYLPVTCLEDTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
BASE COUNT         3741 a         4420 c         4843 g         3931 t
ORIGIN      
        1 tacagaaaat aattatgggt tttttatttg tttgatttat aggtttgatg aatatttctc
       61 ttaaatagag tgaatgttgc attcagtgga aggaatttac aaacttcagc ttcggataat
      121 tctcaccaac tggtaaagtg agcgccgtgg agattttccc ctattggact cattttcagg
      181 ttatacccca tgtcatcatt gaatattaaa cagggaagtg acgctcattt ttctgagtat
      241 cctctggcgt cgcccagtaa taatgaaatt gatttactta atctaatcga agttttatgg
      301 cgggccaaaa aaacggtcat ggcggtcgtt tttgcatttg cctgcgcagg tttactgatc
      361 tctttcatcc tgccgcaaaa atggaccagc tcagccgtta ttactcccgc cgaagccatt
      421 cagtggcagg acctggagaa aacctttacc aaactccgcg ttcttgatct cgatgttaat
      481 atcgaccgtg gcggtgcatt taacctgttt atcaagaagt ttcagtctgt tagcttgctg
      541 gaagagtacc tgcgttcatc accttatgtg atggaccaat taaaagaggc gaaaatcgat
      601 gaactggatt tgcatcgcgc tattgttgca ttgagcgaaa aaatgaaagc ggttgatgac
      661 aatgccagta agaaaaaaga tgaaccgtca ctgtatacct cctggacgct aagttttacc
      721 gcgccaacca gtaaagaggc gcagaccgtt ttgagcgggt atatcgatta tatctctgcg
      781 ttggtggtga aagagtcgat agaaaacgtc cgtaataaac tggagatcaa aacccagttt
      841 gaaaaagaaa aactggctca ggatcgcatt aaaacgaaaa atcaacttga tgcaaacatt
      901 cagcgcctca attattcact cgacattgcc aacgcggcag gaattaaaaa gcccgtatac
      961 agtaatggtc aggccgttaa agatgacccc gatttctcta tttctctcgg cgcagacggt
     1021 attgaacgca aactggaaat tgaaaaagcg gttactgacg ttgcggaact gaacggtgaa
     1081 ttacgtaatc ggcagtatct tgtcgagcaa ttaacaaaag caaatgtcaa cgatgtgaat
     1141 tttacgccgt ttaaatatca gttaagcccg tcattgccag tgaaaaaaga cggtccgggt
     1201 aaggcgatta ttgtgatcct ttccgcgctg atcggcggga tggtggcttg tggtagcgtg
     1261 ttattgcgct atgcgatggc atccagaaaa caggatgcca tgatggcaga ccacttagtt
     1321 taactatttg cggttgaagg tgccgttcgt ccgagcggca ccacaagcgg cgttccggct
     1381 accggatcgt caatgatcat gcatcgcaga ccataaatgc gctcaatcag ttcagcagtg
     1441 acaatctcct tcggcgctcc ctgagcaaca attttccctt cccgcaatgc aatcaaatgg
     1501 ctggcgtaac gacaggcctg attaagatcg tgcagcaccg ccgccagggt atagcctttc
     1561 tcgcggttca gttcgcttaa cagttccagt agatcaatct gatgactgat atccagccag
     1621 gtcgtgggtt cgtcgagcag cataattgcc gtttcctggg ccagcaccat cgcgatccac
     1681 gctcgctggc gttgtccgcc agaaagggta tccacgcttt gatctgccag atgagttatt
     1741 cccgtggcct gcatcgcttt cgttaccgct tcttcatcct ctttgcgcca gcgggtaaac
     1801 agcggttgat gcggataacg tccacgcgcc accagctcct gcacggtgat atcgcccggc
     1861 gtggtagcat tttgcgccaa cagaccaatc cggcgtgcaa cttctttact ggcgtaatgt
     1921 tgaatgtgct cgccatccag ccagacatgc ccgtgagcag gcgtcatcag gcggcttaag
     1981 gtacgcagta acgtggattt accacagcca tttggtccga taattgccgt gaagtgacca
     2041 tcaggaattt ctacagtcag attttccgca acggtatatt tgccatatcc cagggttaac
     2101 tgttcgccgc gcaaacgggc tactgattcg gtcatttttt gcgagactcc tgaattaaca
     2161 agacgataag gtaaataccg ccgaggctga cggtaacgac gccaaccgga agttgatacg
     2221 gcataaacag ttgttgggcg cagagatcgg ccgccagcag taacagcgct ccgcacagcg
     2281 ccgcctgggt cagcccccag cgagcggtgc cgctaatgcg tcgggcaatg tgcggcgcga
     2341 ctaaagcaat aaaggaaatc ggcccggcaa gcgccgttgc cgcagcggta agcaccactg
     2401 caaccagcat cattaacaga cgcgaacgct cgacgctgac gcccagcgcg caggcggtat
     2461 catcgcccat ctccagcaag cgcattcgcc gcaccagcaa ggtggcggca atgagcatca
     2521 atataatgat gggtgcggaa ggcgaggttt ttgcccacgt caggccgttg agcgatccgg
     2581 cattccacaa acctgctgtt agcgccgttt ctaaagacgc tttcagcaac agccaggtat
     2641 taaaggccac cagcatggcg cgaacgccga taccgataat aatcaaccga aaggtgtcga
     2701 taccgttacg ccaggcgagc agccagacca acagcgaagt gataatgccg cccaccattg
     2761 ctgccagcgc gatagccgtc agatcctgac caaacagcac catcgccacc agcacgccgc
     2821 tccacgcccc ggtgttaaag cccattacgt cagggctgcc gagcgggtta cgcatcagcg
     2881 actgaaaaat cgcgccgctg acgcccagcg ccgcgccaat caacagcgcc atcagcacgc
     2941 gtggtaaacg ccattcggtg accaccatcg tcatactgcg cggcgcatcg cccatcaacg
     3001 cggcgaatac ctgcgagatt tccagcgtga cggcaccgct gcgtaatccc cagatacctg
     3061 caaccacaca ggcggaaatc agcagcaaac aggtgatgag taatcggcga gagacgtaaa
     3121 tcatgcacca cctcgcgttt tacgtcgcac gaggaagatc agcaccggtg caccaataaa
     3181 cgcactgacc acagaaacgc gcagttcgcc gggcacaatc acccgcccga tgatatcggc
     3241 aaacagcagc agggcagggg tagcaagtag cgtgacgggc agcgaccagc gatgatcggc
     3301 acctaccagc cagcgcgcca tgtgcggcat catcaggccg ataaaggcaa tcgggccaac
     3361 taccgccgtc gcactaccac aaagcacggt aatcgccagc agaccaatca actgtgtgcg
     3421 cgccacgcga ctgcccagcg ccgtcgcggt gtcactgccg aggctcaaac tgttcagcgc
     3481 gcgactcagt aatagcgcag ttgctccggc gatcagaacc gggatcagca ccacttttaa
     3541 ggtatgtaga ttgcgaatat ccagcgaacc ggcttgccag aaacgcagtt gatcgtagac
     3601 gtcaggatta agcagggcga tgccgctggt cagtccttcc agcaccgccg ccagcgccac
     3661 gcccgccagg gttaaacgca ccggacttaa ctgccctcca ccctgactgc cggtaaaggc
     3721 aacaatcaat gaggccacca gcgccccggc gaaggccatc gccagttgtt cctgtgcgga
     3781 tgagtaacca aacagcgccg cacccagcac aatggcaaag ctggctccgg cgttcacgcc
     3841 aagcaagccg gggtcggcaa gtgggtttcg ggtgagggtt tgcattaacg ccccggcaag
     3901 gccaagcgcg ccgcctgcca gtaaaccggc aagggtacgc ggcagccgcg cgtcgagcac
     3961 gatggtgcag tcggcgctct ggcaggtgcc ggagaaggcc tccagcacta cggaagcggg
     4021 gagtgatttt gccccaatga gcaggcttaa tgccgtcgcg ataatcagta ataacagcaa
     4081 tccgggcacg gcaatggcgc gtgtcacggc aacagaacca gacatacgaa cttccatgat
     4141 aatgaaatta attatcgtta tcgatcttat ttggatatgt tagcatgtgc agcctaagaa
     4201 taggtattta aaatatttga tggcaaggca ttgtaatgaa taaacaatcc tggctgctta
     4261 acctcagcct gttgaaaacg cacccggcgt ttcgcgcagt attcctcgct cgttttatct
     4321 caattgtgtc tctgggtttg ctcggcgtcg cggtgccggt gcagatccag atgatgaccc
     4381 attctacctg gcaggtgggg ctttcggtga cgctgaccgg cggcgcgatg tttgttggcc
     4441 tgatggttgg cggtgtgctg gcggatcgct atgaacgcaa aaaagtgatt ttgctggcgc
     4501 gcggcacctg tggcattggc ttcattggac tgtgccttaa tgcactgctg ccggagccgt
     4561 cattgctggc aatctattta cttggtttat gggatggttt tttcgcatca cttggtgtta
     4621 cggcgctact ggcggcaaca tctgcactgg tagggcgtga aaacttaatg caggccgggg
     4681 cgatcaccat gttgaccgtg cgcctggggt cggtgatttc gcccatgatt ggcggtttat
     4741 tgctggcgac cggcggcgta gcctggaact acgggctggc ggcggcgggc acgtttatta
     4801 ccttgttacc gttattaagc cttccggcgt tgccaccgcc accgcagccg cgtgagcatc
     4861 cgttgaaatc attactggca ggatttcgtt ttctgcttgc cagcccgctg gtgggcggga
     4921 ttgcgctgct gggtggttta ttgacgatgg cgagcgcggt gctggtactg tatccggcgc
     4981 tggctgacaa ctggcagatg tcagcggcac agattggttt tctctacgcg gcgatcccgc
     5041 tcggcgcggc tattggtgcg ttaaccagcg ggaagctggc acatagtgcg cgaccagggt
     5101 tattgatgct gctctccacg ctgggatcgt tcctcgccat tggtctgttt ggcctgatgc
     5161 cgatgtggat tttaggcgtg gtttgtctgg cgctgttcgg ctggctgagc gcggttagtt
     5221 cgttgctgca atatacaatg ctgcaaacgc aaaccccgga agcgatgtta gggcggatta
     5281 acggtttgtg gacggcgcag aacgtgacgg gcgatgccat aggcgcggcg ctgctgggtg
     5341 gtttgggcgc gatgatgaca ccggttgctt ccgcaagcgc gagcggtttt ggtttgttga
     5401 ttatcggcgt gttgttattg ctggtgctgg tggagttgcg acgttttcgc cagacgccgc
     5461 cgcaggtgac agcgtccgac agttaatgct taaaacagcg ccttaagccg ttccagcact
     5521 tgcatggcgc tgtagtaatc caggcggaac gtctcggttc ccagcgcata aacctgcttg
     5581 ttttgtaccg caggcaggtg cgcgagcagt ggattggcat aaatcgcatc ggcatctttc
     5641 tgatcaccag cgaacaggaa tagcgattca ccgtttaacc ccgcagccag attttcccca
     5701 ccaagctgaa tgatgtcatg gcgtttgccc tggctttggc tggcgtttaa ccctgcgggc
     5761 aacttcgcca gcgtaaagcc gagttgttcc agcatctgcc cttgtgctga ttccggcgtc
     5821 cagagattgg cactgtgtgc agcggcggta tagacaatgg cagtgaccgg ctgcggcggt
     5881 aatttgattt gctctttcgc cgctgccagt tgcttatcaa actgtgcaat ccgctctgcc
     5941 gcttgtttct catgcccggt aatttcgcca agctgcgtta acagcgactg ccagcttttg
     6001 tcgtcgtaat tgatgattaa tgtcggggcg atggtggaaa gctgatcata cagcgccagc
     6061 gccgaatccc cgccggttgc gctaattaaa atcagatccg gcatttgcgc ggcaacggct
     6121 tcggcgctcg gttcgccgat atagagctgt tgcagtttgc gttctttcgc caccttgctc
     6181 cactggcgta aaaagccctg gtcatctgcg acgcggttat tcggcgtggc cgcgccgctg
     6241 gcgatcaccg gagcatcaat cgccagcagt gatccggtca gggtgacgct ggtggaaaca
     6301 atacgctgcg gctggctttc cagggtatga gtgccccggc tgtcagtaat ctgacgcggc
     6361 cagtcggcgg cctgaactac ggctattcct gaaagcaaaa gtcctgttaa tagaagggcg
     6421 ttgcggtgga gcggggcgag tctcacaagt cagcttcctg ttattaataa ggttaagggc
     6481 gtaatgacaa attcgacaaa gcgcacaatc cgtccctttg gggagagggg gcaaaacggc
     6541 gcaggacatc acattgcgct tatgcgaatt catcaataat gcttctcatt ttcattgtaa
     6601 ccacaaccag atgcaacccc gagttgcaga ttgcgttacc tcaagagttg acatagtgcg
     6661 cgtttgcttt taggttagcg gccgaaaata taaatgataa tcattattaa agcctttatc
     6721 atttgtgggg gatgatatgg atacgtcact ggctgaggaa gtacagcaga ccatggcaac
     6781 acttgcgccc aatcgctttt tctttatgtc gccgtaccgc agttttacga cgtcaggatg
     6841 tttcgcccgt ttcgatgaac cggctgtgaa cggggattcg cccgacagtc ccttccagca
     6901 aaaactcgcc gcgctgtttg ccgatgccaa agcgcagggc atcaaaaatc cggtgatggt
     6961 cggggcgatt cccttcgatc cacgtcagcc ttcgtcgctg tatattcccg aatcctagca
     7021 gtcgttctcc cgtcaggaaa aacagacctc agcccgctgt tttacccgca gccagtcgct
     7081 gaacgtggtg gaacgccagg caattcctga acaaaccacg tttgaacaga tgggtgcccg
     7141 cgccgccgca cttaccgcca cgccgcaggt cgacaaagtg gtgttgtcac gattgattga
     7201 tatcaccact gacgccgcca ttgatagtgg cgtattgctg gaacggttga ttgcgcaaaa
     7261 cccggttagt tacaacttcc atgtcccgct ggctgatggt ggcgtcctgc tgggggccag
     7321 cccggaactg ctgctacgta aagacggcga gcgttttagc tccattccgt tagccggttc
     7381 tgcgcgtcgt cagccggatg aagtgctcga tcgcgaagcg ggtaatcgtc tgctggcgtc
     7441 agaaaaagat cgccatgaac atgaactggt gactcaggcg atgaaagagg tactgcgcga
     7501 acgcagtagt gagttacacg ttccctcctc tccacaattg attaccacgc cgacgctgtg
     7561 gcatctcgca actccctttg aaggtaaagc gaattcgcaa gaaaacgcac tgactctggc
     7621 ctgtctgctg catccgaccc ccgcgctgag cggtttcccg catcaggccg cgacccaggt
     7681 tattgctgaa ctggagccgt tcgaccgcga actgtttggc ggcattgtgg gttggtgtga
     7741 cagcgaaggt aacggcgaat gggtggtgac catccgctgc gcgaagcagc gggaaaatca
     7801 ggtgcgtctg tttgccggag cggggattgt gcctgcgtcg tcaccgttgg gtgagtggcg
     7861 cgaaacaggc gtcaaacttt ctaccatgtt gaacgttttt ggattgcatt aaggagcgag
     7921 gatgagcatt ccattcaccc gctggccgga agagtttgcc cgtcgctatc gggaaaaagg
     7981 ctactggcag gatttgccac tgaccgacat tctgactcgc cacgctgcga gtgacagcat
     8041 cgcggttatc gacggcgagc ggcagttgag ttatcgggag ctgaatcagg cggcggataa
     8101 cctcgcctgt agtttgcgcc gtcagggcat taaacctggt gaaaccgcgc tggtacaact
     8161 gggtaacgtc gctgaacttt acattacctt tttcgcgctg ctgaaactgg gcgtcgcgcc
     8221 ggtgctggcg ctgttcagcc atcagcgtag tgaactgaac gcctacgcca gccagattga
     8281 accggcgtta ctgattgccg atcgccagca tgcgctattt agcggggatg atttcctcaa
     8341 cacatttgtt gcagagcatt cttccattcg cgtggtgcaa ctgcacaacg acagcggtga
     8401 gcataacttg caggatgcga ttaaccatcc ggcagagggt tttactgcca cgccatcacc
     8461 tgctgatgaa gtggcctatt tccagctttc cggcggcacc accggcacac cgaaactgat
     8521 cccgcgcact cataacgact attactacag cgtgcgtcgt agcgtcgaga tttgtcagtt
     8581 cacacaacag acgcgctacc tgtgcgcgat cccggcggct cataactacg ctatgagttc
     8641 gccaggatcg ctgggcgtct ttcttgccgg aggaacggtt gttctggcgg ccgatcccag
     8701 cgccacgctt tgcttcccgt tgattgcaaa acatcaggtt aacgttaccg cgctggtgcc
     8761 gccggcagtc agcctgtggt tgcaggcgct ggctgaaggc gaaagccggg cgcagcttgc
     8821 ctcgctgaaa ctgttacagg tcggcggcgc acgtctttct gccacgcttg cagcgcgtat
     8881 tcccgctgag attggctgcc agttgcagca ggtgtttggc atggcggaag ggctggtgaa
     8941 ctacacccgt cttgatgata gcgcggagaa aattatccat acccagggtt acccaatgtg
     9001 tccggacgat gaagtgtggg ttgccgatgc cgaaggaaat ccactgccgc aaggggaagt
     9061 tggacacctg atgacgcgcg ggccgtacac cttccgtggc tattacaaaa gtccgcagca
     9121 caatgccagt gcctttgatg ccaacggttt ttactgttcc ggcgatctga tctctattga
     9181 tccagagggt tacatcaccg tgcaggggcg cgagaaagat cagatcaacc gtggcggcga
     9241 gaagatcgct gccgaagata tcgaaaacct gctgctgcgc catccggcag tgatctatgc
     9301 cgcactggtg agcatggaag atgagctgat gggcgaaaaa agctgtgctt atctggtggt
     9361 aaaagagccg ctgcgcgcgg tgcaggtgcg tcgtttcctg cgtgaacagg gtattgccga
     9421 atttaaatta ccggatcgcg tggagtgtgt ggattcactt ccgctgacgg cggtcgggaa
     9481 agtcgataaa aaacaattac gtcagtggct ggcgtcacgc gcatcagcct gaaggagaga
     9541 acacgatggc tattccaaaa ttacaggctt acgcactgcc ggagtctcac gatattccgc
     9601 agaataaagt tgactgggcc tttgaaccgc aacgtgccgc gttgttaatc catgatatgc
     9661 aggactattt tgtcagcttc tggggcgaga actgcccgat gatggagcag gtgatcgcga
     9721 atattgctgc gctgcgcgac tactgcaaac agcacaatat cccggtttat tacaccgccc
     9781 agccgaaaga gcagagcgat gaagatcggg cgctgttgaa tgatatgtgg gggccgggcc
     9841 tgacccgctc gccggaacag caaaaggtgg tggatcgcct gacgccagat gccgacgaca
     9901 cggtgctggt gaagtggcgc tacagcgcgt ttcatcgttc tccgctggag caaatgctca
     9961 aagagagtgg acgtaaccag ctgattatta caggggtata tgcccacatt ggctgtatga
    10021 ccaccgcaac cgacgcattt atgcgcgata ttaaaccgtt tatggtggcg gatgcgctgg
    10081 ctgatttcag ccgtgacgag catttgatgt cgctgaaata tgtggccgga cgttctggcc
    10141 gggtggtgat gaccgaagaa ttactgccag cacctatccc cgccagcaaa gcggcgctgc
    10201 gtgaggtgat tctgccgttg ctggacgagt ccgatgaacc gttcgatgac gacaacctga
    10261 tcgactacgg tctggattcg gtgcgcatga tggcgctggc ggcgcgctgg cgcaaagtgc
    10321 atggtgatat cgactttgtc atgctggcga aaaacccgac catcgacgcc tggtggaagc
    10381 tactctcccg cgaggtgaaa taatggattt cagcggtaaa aatgtctggg taaccggcgc
    10441 aggtaaaggt atcggctacg ccacggcgct ggcgtttgtt gaggcgggag cgaaagttac
    10501 aggttttgat caagcgttca ctcaggagca atatcccttt gcgaccgaag taatggatgt
    10561 tgccgacgcc gggcaggtgg cgcaggtatg tcagcgactg ttagctgaaa cggagcgact
    10621 ggacgtgctg atcaatgcgg cgggaatttt gcgcatgggc gcgaccgatc agctcagtaa
    10681 agaggactgg cagcagactt ttgcggttaa cgtcggcggt gcgtttaacc tgttccagca
    10741 aaccatgaac cagtttcgcc gtcagcgggg cggggcgatt gtcactgtgg cgtccgacgc
    10801 cgcacacacg ccgcgtattg gtatgagtgc ttatggcgca tcgaaagcgg cgctgaaaag
    10861 cctggcgttg agcgtcgggc tggaactggc gggtagcggc gtgcgctgta atgtggtttc
    10921 gcctggctcc accgacaccg atatgcaacg cacgctgtgg gtgagcgatg acgccgaaga
    10981 acagcgtatt cgcggctttg gcgagcagtt taaactcggc attccgctgg ggaaaatcgc
    11041 ccgtccacaa gagatcgcca acacgatttt gttcctcgcc tctgacctcg ccagccatat
    11101 caccctacag gatattgtgg tcgatggcgg ctcaacgctg ggggcataag catgatctgg
    11161 aaacgccatt taacgctcga cgaactgaac gccaccagcg ataacacaat ggtggcgcat
    11221 ctgggaattg tgtatacccg tctgggagat gatgtgctgg aagccgaaat gccggttgat
    11281 acccgtactc atcagccgtt tggcctgctg catggcggcg cgtcggcggc gctggctgaa
    11341 acgctgggat cgatggccgg atttatgatg acccgtgacg ggcagtgtgt ggtgggcacg
    11401 gaacttaacg ccacccatca tcgcccggtg tctgaaggca aggtacgcgg cgtctgccag
    11461 ccgctgcatc ttgggcggca aaatcagagc tgggaaatcg tcgttttcga tgaacagggg
    11521 cggcgttgct gcacttgtcg gctgggtacg gcagttttgg gatgagcccg acagaattaa
    11581 atgagattgc aggaaaactc ggttaacgga gtgatcgagt taacattgtt aagttaaata
    11641 ttggtttcaa ctccgattta catggttacc gtgttgttaa atcgcacaaa gatgttatag
    11701 aaacaaaatg taacatctct atggacacgc acacggataa caactatgaa caaatcaggg
    11761 aaatacctcg tctggacagt gctctctgta atgggagcat ttgctctggg atacattgct
    11821 ttaaatcgtg gggaacagat caacgtgctg tggattgtgg tggcgtcggt ctgtatctat
    11881 ctgatcgctt accgttttta tgggctgtat atcgccaaaa atgtgctggc ggttgacccg
    11941 acgcgtatga cgccagcggt gcgccataac gacgggctgg actatgtgcc gacggacaag
    12001 aaagtgctgt tcggtcacca ttttgcggcc attgccggag cagggccgct ggtggtgccg
    12061 gtgctggcag cgcaaatggg ctacctgccg gggatgatct ggctgctcgc cggggtggtt
    12121 ctcgccggtg cggtgcagga tttcatggtg ctgtttgttt ctacgcgccg tgacggtcgc
    12181 tcgctgggtg agctggtcaa agaagagatg gggccaactg ccggggtgat tgcgctggta
    12241 gcctgcttta tgatcatggt cattatcctt gcagtactgg cgatgatcgt ggtgaaagcc
    12301 ctgactcata gcccgtgggg aacgtatacc gttgcgttca ccattccgct ggcgattttt
    12361 atgggaatct acctgcgcta tctgcgtccg ggccgcattg gcgaagtgtc agtgattggc
    12421 ctggtgttcc tgattttcgc cattatctct ggcggctggg tggcagaaag tccgacctgg
    12481 gcaccgtact ttgactttac cggcgtgcag ctgacctgga tgctggtggg ctacggtttt
    12541 gtggcggcgg tgctgccggt gtggttgctg ctggccccgc gtgactatct ctcgaccttc
    12601 ctgaaaatcg ggactatcgt cggtctggcg gtaggcattt tgattatgcg cccgacgctg
    12661 accatgcctg cgctgaccaa atttgtcgat ggcactggcc cggtatggac cggtaacctg
    12721 ttcccgttcc tgtttatcac catcgcctgt ggtgcggtgt ctggcttcca tgcgctgatc
    12781 tcttccggca ccacgccgaa gatgctggcg aacgaagggc aggcgtgctt tatcggctac
    12841 ggtgggatgt tgatggaatc cttcgtggcg attatggcgc tggtttctgc ctgtatcatc
    12901 gatccgggcg tgtattttgc catgaacagc ccaatggcgg tgctggctcc ggcagggacg
    12961 gcggatgtag tcgcttctgc cgcgcagatg gtgagtagct agggctttag cattacacca
    13021 gatacgctca accagattgc cagcgaagtg ggtgaacagt cgatcatttc ccgtgcgggc
    13081 ggtgcgccga cgttggcggt ggggatggct tacatcctgc acggtgcgct gggtggtatg
    13141 atggatgtgg cgttctggta tcacttcgcc attctgtttg aagctctgtt tattctgacg
    13201 gcggtagatg cgggtacgcg tgctgcgcgc tttatgttgc aggatctgct gggcgtggtg
    13261 tctcctggcc tgaagcggac cgattcactg cctgctaacc tgctggcaac ggcgctgtgc
    13321 gtactggcgt ggggctactt cctccatcag ggcgtggtcg atccgctggg cggtattaac
    13381 actctgtggc cgctgtttgg tattgccaac cagatgctgg cagggatggc gctgatgctc
    13441 tgtgccgtgg tgttgttcaa gatgaaacgt caacgttacg cctgggtggc gctggtacca
    13501 acggcctggc tgctgatttg taccctgacc gctgactggc agaaagcgtt tagcccggat
    13561 gcgaaagtcg gcttcctggc cattgctaac aagttccagg caatgatcga cagcggcaat
    13621 attccgtcgc agtatactga gtcacagctg gcgcaactgg tgttcaacaa ccgtctggat
    13681 gccgggttaa ccatcttctt tatggtggtc gtggtggttc tggcactgtt ctcgattaag
    13741 acggcacttg cggcattgaa agatccgaag ccaacggcga aagaaacgcc gtatgagccg
    13801 atgccggaaa atgtcgagga gatcgtagcg caggcgaaag gcgcgcacta aagtcagagt
    13861 gaggggggat gttggcgaat gttggcatag tgccgagggt tccccctcac cctaaccctc
    13921 tccccgatgg ggcgagggga ctgaccgagt gcgttgatag catttgtagg ccggataaag
    13981 cgttcacgcc gcatccggca ctctttcagc aacatggtta gcggaggcca agatgtttga
    14041 ttcactggca aaagccggaa aatatttagg tcaggcggcg aagctgatga ttggtatgcc
    14101 tgattacgac aactatgtcg aacatatgcg ggttaatcat cccgatcaaa cgccgatgac
    14161 ctacgaggag tttttccgtg agcggcagga cgcgcgctac ggtggaaaag gcggcgcgcg
    14221 ctgctgctaa atttgcacgt caggctttaa acgattccac tttttcgaac gctgcacgca
    14281 acgtatctgg tgtcagcgtg actggcaggt aatgaatgga ctccaccggg cgcaaggtgt
    14341 gggcaatcac tttgtcgatc tccgcctgat tattgatatc cacctccagc tccgccagcg
    14401 tagtcggcag atgaaaacgc tgatacgccc cggttaattg cgccagcaca tcatcctgac
    14461 ccagcaaggc gctttgcacc agaattccgt aagcgacttt ggtgccgtga agaaacttct
    14521 cggtttgcgg caggacagtc agaccgttat gcacggcatg agccgccgcc acacgcgtaa
    14581 aacgatcgcc cagaccaccg accatcccac caccagcaat aatagcatcc accacatcgc
    14641 aaaatgattg cgttaactgc tgattttgct gatcggcaag tgcctgttcg ctactgttta
    14701 acaagacgtc gcgaatggcc tgcgcattat tgatccccag ccgcacggtt agcggcaacg
    14761 tttctggttg cggagccagc accaccgctt cataccattt cgccagcgtg tcaccgatcc
    14821 ccgccagcag atattgttgc ggtgcattga ggataatctc cggttccacc agcaccataa
    14881 aattggcgtc gtcgaaaatc tcataatgca gcgcctgtcc ggcatcgtta taccagacgg
    14941 agagcggtgt ccaggctgcg caggtggcgg cgatcgtcgg agcggcaaca aatggcagac
    15001 cgagacggcg ggcgagggct ttcgcggtgt cgagcagtgc gccgccgccg acgccaatca
    15061 ccacgctgcg atcgtcaccg gactcagccg ccagttgctg tacatcgctt tcgctgcaat
    15121 gaccacgaaa caaaatatgc tttgcccctg gaagtccaaa cgctggcgga agtttggttt
    15181 gcgccgcagc aatggcgcgt ttgccgtaga tccacaccgc gcgagaaagt tgttcatcag
    15241 tgaaaaaatt gtgcaggtga ttgaaacttc ctggatgtga aaagtagtta gccgggccga
    15301 cgaccacgcg gataggattg tgaggcatgt tgtgttgtcc ttattgtttt atttagacat
    15361 ctaaacgtct tgattgccaa atactagcat cgtgttatag tgccttcaac acgcaacttc
    15421 gtcaggtaca ataaaaatga caaataaccc tctgattcca caaagcaaac ttccacaact
    15481 tggcaccact attttcaccc agatgagcgc gctggcgcag caacaccagg cgattaacct
    15541 gtcgcaaggc tttcctgatt ttgatggtcc gcgctattta caggagcggc tggcgtacca
    15601 cgttgcccag ggggcaaacc aatacgcgcc catgaccggc gtgcaggcct tgcgcgaggc
    15661 gattgctcag aaaacggaac gtttgtatgg ctatcaacca gatgccgata gcgatatcac
    15721 cgtaacggca ggggcgacgg aagcgttata cgcggcgatt accgcactgg tgcgcaatgg
    15781 cgatgaagtg atttgttttg atcccagcta tgacagttac gcccccgcca tcgcgctttc
    15841 tgggggaata gtgaagcgta tggcactgca accaccgcat tttcgcgttg actggcagga
    15901 atttgccgca ttattaagcg agcgcaccag actggtgatc ctcaacactc cgcataaccc
    15961 cagtgcaact gtctggcagc aggctgattt cgccgctttg tggcaggcga tcgccgggca
    16021 cgagattttt gtcattagcg atgaagtcta cgagcacatc aacttttcac aacagggcca
    16081 tgccagtgtg ctggcgcatc cgcagctgcg tgagcgggca gtggcggttt cttcatttgg
    16141 caagacctat catatgaccg gctggaaagt gggttattgt gttgcgccag cgcccatcag
    16201 cgccgaaatt cgcaaggtac atcagtatct gaccttttcg gtgaataccc cggcacagct
    16261 ggcgcttgct gatatgctac gtgcagaacc tgagcattat cttgcgttac cggactttta
    16321 tcgccagaag cgcgatattc tggtgaatgc tttaaatgaa agccggctgg agattttacc
    16381 gtgtgaaggt acatactttt tgctggtgga ttacagcgcg gtttctaccc tggatgatgt
    16441 tgagttttgc cagtggctga cgcaggagca cggcgtagcg gcgattccgc tgtcggtgtt
    16501 ttgcgccgat cccttcccac ataaactgat tcgtctctgt tttgccaaga aggaatcgac
    16561 gttgctggca gcagctgaac gcctgcgcca gctttagcta tttaaccgtc caggcgcggg
    16621 aatattgacg gtcggcaaac aactcttgca ggccgttaat ttgcttcagg cgcagcactt
    16681 cgtcgctgtc cattcccagc tctttgccaa ttttattatc gtcccatccc aactggctaa
    16741 gttcacggac gatttccgac atggcggtga tttgatggcg tccacgagcg cgattatgac
    16801 gaatagtcgc ggcgatgcgt tgattacgcg tatcttccag acaggtcacc ggcaaataac
    16861 cttttaagcg taattttaat gatgatgagc ctttaccaat ttcatggtgg tgaaaaccat
    16921 ccacgatttc catgg
//
LOCUS       DADLSB010000084        16896 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.084, whole genome shotgun sequence.
ACCESSION   DADLSB010000084 DADLSB010000000
VERSION     DADLSB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.084"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JN331_002570"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JN331_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7260689.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JN331_002571"
     CDS             2219..2767
                     /locus_tag="JN331_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY7260690.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JN331_002572"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JN331_002572"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY7260691.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JN331_002573"
     CDS             complement(3664..4854)
                     /locus_tag="JN331_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY7260692.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN331_002574"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN331_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY7260693.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JN331_002575"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JN331_002575"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY7260694.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JN331_002576"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JN331_002576"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY7260695.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12379
                     /gene="pepN"
                     /locus_tag="JN331_002577"
     CDS             9767..12379
                     /gene="pepN"
                     /locus_tag="JN331_002577"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY7260696.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="JN331_002578"
     CDS             complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="JN331_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7260697.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGPDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="JN331_002579"
     CDS             complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="JN331_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAY7260698.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
     gene            complement(14153..15298)
                     /gene="ssuD"
                     /locus_tag="JN331_002580"
     CDS             complement(14153..15298)
                     /gene="ssuD"
                     /locus_tag="JN331_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY7260699.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15295..16254)
                     /locus_tag="JN331_002581"
     CDS             complement(15295..16254)
                     /locus_tag="JN331_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7260700.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRTGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="JN331_002582"
     CDS             complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="JN331_002582"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY7260701.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVL
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4217 a         4373 c         4326 g         3980 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgtacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgcgatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cgctcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagccca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgat
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgcaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccaacgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagag ggcgcactgg tcatcagtaa
    10021 tttgccggag cgttttacgc ttaagatcat taatgaaata agcccggcgg cgaatacggc
    10081 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
    10141 ccgccatatt acgtattatc tcgaccgccc ggacgtgctg gcgcgtttta ccaccaaaat
    10201 tattgccgat aaaatcaaat atcccttcct gctttccaat ggtaaccgcg ttgcgcaagg
    10261 cgaactcgaa aacggacgcc attgggtaca gtggcaggac ccgttcccga aaccgtgcta
    10321 cctgtttgcg ctggtggcag gcgactttga tgtactgcgc gataccttta ccacgcgttc
    10381 tggtcgcgaa gtagcactgg agctgtacgt cgatcgcggc aaccttgatc gcgcgccgtg
    10441 ggcgatgacc tcgctgaaaa actccatgaa atgggatgaa gaacgctttg gcctggagta
    10501 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
    10561 taagggtctg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccaccga
    10621 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
    10681 taaccgagtg acctgccgcg actggttcca gctcagtctg aaagaaggtt taaccgtctt
    10741 ccgcgatcag gaattcagct ctgaccttgg ttcccgcgca gttaaccgca tcaataatgt
    10801 gcgcaccatg cgcggattgc agtttgcgga agacgccagc ccgatggcgc acccaatccg
    10861 cccggacatg gtcattgaga tgaacaactt ctacaccctg accgtttacg agaagggcgc
    10921 ggaagtgatc cgtatgatcc acaccctgct gggcgaagaa aacttccaga aagggatgca
    10981 actctatttc gagcgtcatg atggtagcgc agcgacctgt gacgactttg tgcaggcgat
    11041 ggaagatgcg tcgaatgttg atctctccca tttccgccgt tggtacagcc agtccggtac
    11101 gccggttgtg accgtcaaag acgactacaa tccggaaact gagcagtaca ccctgaccat
    11161 cagccagcgc acgccagcca cgccggatca ggcagaaaaa cagccgctgc atattccgtt
    11221 tgccatcgaa ttgtatgaca acgaaggcaa agtgatcccg ttgcagaaag gtggtcatcc
    11281 ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc tttgtttttg ataatgtcta
    11341 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
    11401 gtggagcgat cagcaactga ccttcctgat gcgtcatgcg cgtaatgatt tctcccgttg
    11461 ggatgcggcg caaagtttgc tggcaaccta catcaagctg aacgtcgccc gtcatcagca
    11521 agggcagccg ctgtctctgc cggtacatgt ggctgacgct ttccgcgcgg tgctgctcga
    11581 tgagaagatt gatccggcgc tggcggcaga aatcctgacg ctgccttctg tcaatgaaat
    11641 ggctgaactg ttcgatatca tcgacccgat tgctattgcc gaagtacgcg aagcactcac
    11701 tcgtactctg gcgactgaac tggcggatga gctgctggct atttacaacg cgaattacca
    11761 gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc actctgcgta atgcctgcct
    11821 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcaatacca
    11881 tgaagcaaac aatatgaccg atgcgctggc agcgctttct gcggcggttg ctgcacagct
    11941 gccttgccgt gacgcgctga tgcaggagta cgacgataag tggcatcagg acggtctggt
    12001 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
    12061 gcgtggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgta ttcgttcgtt
    12121 gattggtgcg tttgcgggca gcaacccggc agcgttccat gccgaagatg gcagcggtta
    12181 ccagttcctg gtggaaatgc ttaccgacct caacagccgt aacccgcagg tagcttcacg
    12241 tctgattgaa ccgctgattc gcctgaaacg ttatgatgcc aaacgtcagg agaaaatgcg
    12301 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatcttt acgagaagat
    12361 aactaaagca ctggcttgat acttttgagc caaaatgcct gatgcgacgc ttgacgcgtc
    12421 ttatcaggcc tacaactccg ttgaaattgc tggttttgta ggctggataa ggcgttcacg
    12481 ccgcatcagg cagccgtgcc caactgcctg atgcgatgct tgacgtgtct tatcaggcct
    12541 acaagtccgt tgaaaatgct ggttttgtgc gccacatccg gcatattaac catgacgccg
    12601 aatcagttgt tcagaatgcc cacgccgcat tacccgttgc aatacttccg cttccagctc
    12661 cgctaatcgc accgacccta atcgacgcgg acgggggata tccactgtca aatccggacc
    12721 aatttttccc tcttcaatta acaacacccg gtcagccatt gctaccgctt cgctcacatc
    12781 atgcgtcacc aacagtacgg taaagccgtg ctgctgccaa agtgacacaa tcaaatcctg
    12841 catctcgagt cgcgttaagg cgtccagtgc cccgagcggt tcatcaagca acaataaacc
    12901 cggtcgatga atcaacgctc ttgccagcgc cacacgctgt ttctgcccgc cagaaagtgc
    12961 ggcaggccat tccccggcgc gattctccag tcctaccgca gccaacgccc gacgcgcggc
    13021 atcgcgccac tgacctttaa ggcctaaccc aacgttatca atcaccgatt tccacggcag
    13081 cagacgcgca tcctgaaaca tcattcgcgt atcatcctga atttcagcca gcggtgtggt
    13141 gcccgctaac acatcgcctg cggttggcgt ttccagaccg gccagcaggc gcagcagggt
    13201 acttttacca ccaccgctgc ggcccaccac cgccacaaac tgacctgccg gaatatgtaa
    13261 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
    13321 tggtgtgccc tggttcagac gagcagtatt catatcgtgg cctccttcaa atgataagcc
    13381 gggttccagc gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc aagtttgccg
    13441 agcagggcgt aaagaataat agcgactacc accacgtctg tttgcaaaaa ctctcgcgca
    13501 ttcatcgcca gataaccaat gccggaattg gcagaaatgg tttcggcaac aatcagcgtc
    13561 agccacatca ggcccaacgc aaaacgcacg ccgaccataa ttgagggcag ggcatcgggc
    13621 aggatcacat ggataaacag cggtatgccg gataatccat agctacgtgc catctccacc
    13681 agcccgcgat cgatattacg gatgccatgc caggtgttga tataaatggg gaacagcgta
    13741 ccgagcgcca ccagaaagat tttcgcggat tcatcaatgc caaaccacaa aatcaccagc
    13801 gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa ttgaggtatc cagcagcctt
    13861 tctccccagc gcgacagtcc gctaatcagt cccagaatca ggcccagcga tccgccaatt
    13921 gaaaagccaa tcagcgcccg ccaggaactg atcgccagat gctgccacag ttcgccgctg
    13981 gcggagagcg tccagaacgc cgtcaccact ccttccggtg aaggcaaaat acgcgtcgac
    14041 agccagccaa ccgaggaggc cagttgccac accgccacga tgcccaccgg taaaaaccag
    14101 ggggcaacgc gcaataacca cttcttcact ggcgttgcca tcatcgtgtt ccttagcttt
    14161 gcgcgacttt acgggggata aaatcattcg ccaccgcttc gccttgcgga ttcagcggct
    14221 gcggctgggg aatttccgga atggcgacat ccagatgtgg gaacagcaac tcgccaaccc
    14281 gatacgcttc ttccagatgc gggtagcccg aaagcacaaa gctgtcgatg ccaagcgcgg
    14341 cgtattcgtt gattcgcgcg gcgaccgtag gaccatcgcc caccagcgcc gttccggcac
    14401 cgccgcgcac taagccaacg cccgcccata aattggggct gatctccaga ttgtcgcgct
    14461 tgccgttatg tagcgccgcc attcgctgtt gtcctacgga atccgtccgg gcgaatgcgg
    14521 cttgtgcttt ggcgatagtc tcatcatcaa gatgcgagat taaccgctcg gcggcctgcc
    14581 acgcttcgtc gttagtttca cgaacaatca catgcagacg aataccgaaa cgaattttgc
    14641 gcccatacgc ggcagctttc gcccgcactt gttcgatttt ctctttaaca agttccggcg
    14701 gttcgcccca ggtgaggtag agatcaactt gttctgccgc cagctcctgg gcgacatctg
    14761 acgatccgcc aaagtaaagc ggaggataag gctgttgaat cggcgggaag agcagttttg
    14821 ctccgcgcac atgaatatgt ttaccgttga aattgacggt ttcccccagc aataagcgcc
    14881 gccagacttg ggtaaattcc gccgaggctt cgtagcgctc gctatgatca aggaacacgc
    14941 cgtcgcctgc cagctcttgt ggatcgctgc ctgtgaccag attaaacaac gcacgaccat
    15001 ttgagagacg gtcaagcgtg gcggcctggc gggcggcaac agtaggtgag gttacgctgg
    15061 gacgcagggc gacaagaaac ttcagccgct gcgtcaccgg gatcatcgac gctgcaacca
    15121 gccacgcatc ttcacacgat cgccccgttg ggatcagcac accggtatag cccagacgat
    15181 ccgccgcttg cgcaatttgt tgcagataac cgtgatcaac cgggcgtgaa ccttcttccg
    15241 ttcccagata gtgcccgtca ccgtgggtcg gtaaaaacca gaacatattc agactcataa
    15301 ttgttttcct tccagttgag tgggctgcca gatgcgctgg cgaatatcga cttttttcgg
    15361 caccagacgg ttttcataaa acagatctgc cgtttgctgc tgtaaggcgg caacctcggc
    15421 gttaaccggt ttgatggtgg taggagggcg atgatctaag taagaggcaa tcaccggtgc
    15481 cggtaagccc atcgtttttg ccagtaaagc gatgctttgt tcgcgctggc tgcgggttaa
    15541 cgcatcggcc tcactaaagg ttgccagtac gccctgaata aaagcgccgt ttttttctgc
    15601 atagggacga gctgccagat aaaacgatcc agtttgattg agatcggtgc cgtctttcag
    15661 cacccgcacg ccgccctgta ataacgcagc ggagtagtag ggatcccaga tagcccaggc
    15721 gtcaacgtta ccttgctgga acgcggcgcg ggcatcggca ggcgtcaggt aagtgggctg
    15781 gatatcggtg aatttaagtc cggcctgacg cagtgcacgc agtaaaaggt tgtgtgaact
    15841 ggaacctttc tgaaaggcaa ctttgtgacc tttaagatcg gctacggttt tgatcgggct
    15901 gttttctgcc accagaatca cttcggcttt gggcttcggt ggctcgacgc ccacatatac
    15961 caaatcagcc ccggcagcct gggcaaagat tggcggaata tccccggtac tgccgagatc
    16021 aatactgcca acgtttaacg cttccaacat ttgcggaccc gcggggaact ccacccagga
    16081 gatttttgat tgcggatagc gtttttccag taactggtgg ctttttgcca gcaccatacc
    16141 aatactgcct ttctgatagc ctgtacgtaa cgcttcaggc gaggattctg cagcaaccgc
    16201 aaaagtagag acgctaagca atcccgccag cgccagttta atgatgttac gcatgggcat
    16261 tacctcgcag agacagaagg tcaggaacct gaacatcgcg gcggtgcaat gcctgccaga
    16321 aagtttctag cgcggtatca agatgggttt gcagatttgg cgtgaactgg ggtctgtgat
    16381 ggtaatcaat taattgtgag tcatcggcaa acacgccgtg caggatctcc tgagctttca
    16441 gtgcgcttaa aactggttta agggcataat cgaccgccag cagatgggcc acggtaccgc
    16501 ccgtcgccag cggtagcaac actttgcctt gcaaagcgcg ctctggcagc aggtcgagca
    16561 gggttttcag cgcgccggaa taggcggctt tatacacagg cgtggcgaca atcagcccat
    16621 cagcctgttg cagctgttcg gtgaaggtct tgagtgccgg actatcgaaa cgagcataaa
    16681 gcagatcttc cggggcgaag ttttgcagat tccagtgata aacctctaca tctaggccat
    16741 ttagtttttc ccgcgcatat tccagcaagg agctggagcg agaaggaaag cgaggactac
    16801 ccgccagggt gatgacacgc atactctctc cttataacca attgttcttt ttttgttaac
    16861 attgataaca attcggtcag tctgtcggag agacaa
//
LOCUS       DADLSB010000085        16807 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.085, whole genome shotgun sequence.
ACCESSION   DADLSB010000085 DADLSB010000000
VERSION     DADLSB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.085"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..597
                     /locus_tag="JN331_002583"
     CDS             <1..597
                     /locus_tag="JN331_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7260702.1"
                     /translation="GAKYLEITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAG
                     VIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMT
                     LTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPV
                     RCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(633..1589)
                     /locus_tag="JN331_002584"
     CDS             complement(633..1589)
                     /locus_tag="JN331_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY7260703.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNHVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1589..2830)
                     /gene="clsB"
                     /locus_tag="JN331_002585"
     CDS             complement(1589..2830)
                     /gene="clsB"
                     /locus_tag="JN331_002585"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY7260704.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPDYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2827..3588)
                     /locus_tag="JN331_002586"
     CDS             complement(2827..3588)
                     /locus_tag="JN331_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7260705.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3721..4131
                     /locus_tag="JN331_002587"
     CDS             3721..4131
                     /locus_tag="JN331_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260706.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4093..5199)
                     /locus_tag="JN331_002588"
     CDS             complement(4093..5199)
                     /locus_tag="JN331_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7260707.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5210..6343)
                     /locus_tag="JN331_002589"
     CDS             complement(5210..6343)
                     /locus_tag="JN331_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7260708.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGMPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6336..8072)
                     /locus_tag="JN331_002590"
     CDS             complement(6336..8072)
                     /locus_tag="JN331_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7260709.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8065..9063)
                     /gene="hlyD"
                     /locus_tag="JN331_002591"
     CDS             complement(8065..9063)
                     /gene="hlyD"
                     /locus_tag="JN331_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY7260710.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPMTVQFGDEAGHE"
     gene            complement(9063..9734)
                     /gene="cecR"
                     /locus_tag="JN331_002592"
     CDS             complement(9063..9734)
                     /gene="cecR"
                     /locus_tag="JN331_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY7260711.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9963..11327
                     /gene="rhlE"
                     /locus_tag="JN331_002593"
     CDS             9963..11327
                     /gene="rhlE"
                     /locus_tag="JN331_002593"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HAY7260712.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIVARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11559..12041)
                     /gene="ybiA"
                     /locus_tag="JN331_002594"
                     /pseudo
     CDS             complement(11559..12041)
                     /gene="ybiA"
                     /locus_tag="JN331_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            12161..14311
                     /gene="dinG"
                     /locus_tag="JN331_002595"
     CDS             12161..14311
                     /gene="dinG"
                     /locus_tag="JN331_002595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY7260713.1"
                     /translation="MALTVALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIAKKKEKTKSPRRRRR"
     gene            14339..15301
                     /gene="ybiB"
                     /locus_tag="JN331_002596"
     CDS             14339..15301
                     /gene="ybiB"
                     /locus_tag="JN331_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY7260714.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15442..16527
                     /locus_tag="JN331_002597"
     CDS             15442..16527
                     /locus_tag="JN331_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY7260715.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
BASE COUNT         3926 a         4450 c         4551 g         3880 t
ORIGIN      
        1 ggagctaagt atctcgaaat tacggcaacc aaatatgatt caccaccaat gtatgtcgcc
       61 atagggttat tattactttg tatgtatggc ttaagtaagg atatcaacaa gataaatgcc
      121 gccatcgcgg gcgtaatata tctgttttta ctctctttgg tggcgattgt cgttgcaagt
      181 ttagttcctg tatatgccat tatcatcgtg ttcagcactg cgggcgcgat gtttttaatc
      241 agtatgctgg ccggtttatt atttaatgtt gatcctggtt ctcaccgttt tatcattatg
      301 atgacgttga cagggttggc cctggtaatc atcgtgaatg cggcattaat gagtgaacgg
      361 cccatttggg taataagttg cttaatgatt gtgttatggc caggcattat ctcgcatgga
      421 cgaaataagc tccttgaact ggcaggaaaa tgccatagtg aagagttgtg gagtccggtt
      481 cgttgcgctt ttacaggtgc attaacactc tattactatt ttatcggctt ctttgggata
      541 cttgccgcga tagctataac gcttgtctgg caaaggcata cgcgtttttt tcattagtcg
      601 taaatgcaga tactaaagaa cttaactaaa cttcacatcg ccgcttcatt tttcgcccgt
      661 agcttcttcg cctggctttc cagtagcagg taacagatca gcgccagcag cagcgggata
      721 aagtaataca ggacgcgata agcgagcagg gcggcgataa tgctcccttt cgaggtatgc
      781 tccccagcca gtagtgcgat aaacaccgct tccagcacac cgatccccgc cggaatatgc
      841 acgatgacgc cagcaatact actaaccagt aacacgccca gtacaaagaa atagttcacg
      901 ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac gctggaaatc
      961 agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc tttgatggtc
     1021 atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat catcagtaag
     1081 ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg caactccacc
     1141 acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt ggtggtaata
     1201 ctgagcgagg aaatccgcgt aatagtgctg cccggtaacc ctagtcggga gtacaaacga
     1261 tagcgcatac caatgccgcc aacccaggta ctgagcgtca ggttgaaggc gtagcagata
     1321 aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa gcgggcgagc
     1381 aggtcatagc agccgtaaat cagatagctg acgaccacca gcccgaccgc actaagcagc
     1441 gcaacgtgat tgtagtcgcg gatgaccttc cagacctctt cccagtccac ttttttggcg
     1501 tagaccacca gcaacacaat caccgcgata aaaaacagcc aggtgaggat cttctttgct
     1561 aagcgccagc gcgggtgtga tttactcatc agggttttac ccccgtgttt tcagtttcta
     1621 cccgatcctg cgtttccatt gtcggttgtg cggggggatc aacctgcgtc agacgtggtg
     1681 tgtgtgccgg aagccagcca accagcgccg ggaagtggcg taaaaagtgg aacgccagca
     1741 cgcttttggt caggttccac caggtgcgtt tgggcagcat ggtttcatcc acctgctgac
     1801 aatctgcggc aataatgccg ttcagattat cgcgcagcgt ctggttaaaa tgacgatcgt
     1861 ggatgatgac atttgcttcg agattcagtg acaaactgag cggatcgaga ttactggacc
     1921 ctactgtcgc ccagtgatcg tccatcaatg ccactttgcc gtggagcggg cggcggcggt
     1981 actcaaaaac ctgaacgccg cctttaacca gatagttata cagcaagcgc gcaccgactc
     2041 tgacaatcgg catatccggt tcgccctgaa tgatcagttt gatccgcacc ccgcgccgtg
     2101 ccgctttacg caaggcgtgt aaaaatcgat agtcggggaa gaagtaggcg ttggcgataa
     2161 tcacttcccg ccgcgcctga gtgagcattt tcagataatg gcgttcaata tcatcgcgat
     2221 gttcttcgtt atcgcgccag accagcaata cctgcgcttc tcccggctgg cggttctctt
     2281 ccgctttgtg atgacgtcgc caccagcgtc gtgccgcgct ctgtccaggc aggttttcca
     2341 gctcaaactg gagaatatct tcgacaatcg gcccttcaag gcgtaccgcg taatcctgtt
     2401 tagcctctgg accgtagctg gacatatgct cggcggagta attcagcccg ccaataaagg
     2461 cgatacgcgc gtcgatcacc acaattttgc gatgcatccg gcgaaacaca ttggtgcgca
     2521 taccaaaaag gcgagggcgg ggatcgtagt agcggaacac tacgccagct gccgtcagtt
     2581 cattgacaaa ctcatcgctg agatccggcg aaccgtagcc atccagcaag acttccgctt
     2641 taaccccgcg ttgcgctgct gccagtagtg ccgcatgcag ctgtttgccg acgttatcct
     2701 caaaccagat aaacgtttca agaatgatgc gttcttgtgc ctcgccaatc gccttaaaca
     2761 ccgcgggata atattgctcg ccgttttcca gcaactggat cttattgcct tcgcgccagc
     2821 tacatttcat aaatgaatct ccgcacttaa aggggcatga tcagaaaggt gtcgccatgt
     2881 ccgcagcggc aacgcggttg gcgcgctggc gctggcattt ttgacgtaga tcctgtccag
     2941 tcgtagtaga ggaaattgca ccggaaacgt gcgcgccggg cgtccgtggg cgcgggtaaa
     3001 aatctcatcc agtccggcct gcacttttaa cggatgatta gctttttgcc gccagtcatt
     3061 gaaatcaccc gccaccaata ccggttcgcc gtccggtagc tcattcaccc attcggcgag
     3121 catcgcaagc tgcgcctgac ggtgcgcctc acgcaggccc agatgtacgc acatcacatg
     3181 aatcgctttt ccggtcatcg gcggcacaat gcggcagtag agcacgccgc gcttttccgc
     3241 accatcgacc gaaacatcgc gattctcata atgttcaatg ggataacgcg acagtacggc
     3301 gttgccgtga tgcccttccg ggtatacggc attgcgaccg taggcaaaat cgctccacat
     3361 agtgtcggcg agaaactcgt agtgcgaggt atcgggccag ttttccacat gcagcggatg
     3421 aacttcgtgc gcgcccatca cttcctgcag gcaaacaata tcggcgctga cggtacgcac
     3481 ggcatcgcga agttccggca aaatgaagcg tcggttaaac gcggtaaagc ctttgtgaat
     3541 gttgatggtg agcaccttga acgaaaattg ttgtgtttga tcgggcataa ttttcctgtc
     3601 tttgcctctt atctcattga aatagtgtag tcggcgtcac aaaaaggtgc ggtcttacgg
     3661 aattttccgt aaagttcggt actctgagta agtagagata aattcttcag gagagaagcc
     3721 atgaagtggc aacaacgtgt tcgtgtcgca acgggtctaa gttgctggca gattatgttg
     3781 catttactgg tagtggcgct gctggtggtg ggctggatga gtaagactct ggttcacgtc
     3841 ggcgtgggat tatgcgcact gtattgtgtc acggtagtga tgatgctggt gtttcagcgc
     3901 caccccgagc aacgctggcg tgaggtggca gacgtgctgg aagagctgac cacgacctgg
     3961 tattttggcg cagcgctgat tgtgctatgg ctgttgtccc gcgtactgga aaacaacttt
     4021 ttgctggcaa ttgcagggct ggcgatcctt gctggcccgg cggtagtgtc tttgctggcg
     4081 aaagataaga agttacatca ccttacgtct aaacatcgcg tacgccgctg accctgtcgt
     4141 ggccgttatc accagtagcg gccacaaact attccacaca atatccagac tcgcatcctt
     4201 caaataaatc tgcttggtaa tgtccgtaaa gtggcgaata gggttaatcc acgtcagatt
     4261 ttgcagccac actggcatgt tttccaccgg cgaaacgtaa ccggaaagga gaatggcggg
     4321 catcataaaa acaaacacgc cgataaacgc ctgctgttgt gtcgagcaga gtgatgaaat
     4381 caacagaccg aatcccacca gcgataaacc gtaaatcacc atcgtaaagt agaacagcgc
     4441 tagcgatccg gcgaagggga tttgatacgc ccagatacca atcgccagca caatggtggc
     4501 ctggaaggtg gcgacaatta acgccggtac ggctttgccg atgaagatct gccaggtggt
     4561 gagcggcgaa accagtagct gatcgagcgt accttgttca cgttcgcggg cgacggaaag
     4621 tgaagtgacg atcattacgc cgatagtggt gatcatggcg atcagcgacg gcaccacaaa
     4681 ccatttgtag tcgagattcg ggttatacca gttgcgtacc accagctcgc tgttgttggg
     4741 tttcggtttt ccttccagca gctcctgctg ataatttttg acgatctgtt gcaggtagtt
     4801 ggcggcaatt tgcgcactgt tggagttacg cccgtcgagg atcaactgca acggcgcggt
     4861 ctggaaggta tccagtttgc gcgagaagtc agccgggaaa cgcaccagta gtaacgcctt
     4921 ttgtgtgtcg atggtcgggc ggatctcctg tgggcttttc agcagcagca catgtgtaaa
     4981 ggcgctggcg cgggcaaaac gttgagtaag ttccaccgaa tgctcgccgt tatcttcatc
     5041 gtagatggcg atggtggcgt tagtcacttc cagcgtggcg gcgaacggga acaggatcac
     5101 ctgaattagc acgggtaaaa tcagaatcgc gcgggtttgt ggttcgcgca gcaacgactg
     5161 caactcttta cggattaacg tccataagcg atgaaacatg ctcttctccc taatccagcc
     5221 gacgtttggt tttcagccac gtcaggccga taaacatcac cgccgaagcg atcaaaaaca
     5281 gcacgtttac caccagcacc actggaatat tcccggcgag gaacaggctt tgcagggtgc
     5341 tgacgaaata acgagcggga ataatgtacg tcaccgcgcg gatcaccgcg ggcatactgt
     5401 cgatctgaaa aataaagccg gaaagcataa tcgacggcag aaaagcggcg ttcagggcga
     5461 cctgagcggc attgaactgg ttgcgggtaa tcgtggaaat cagcagcccc attcccaggg
     5521 tactgagtaa aaacaggctg gagataaaaa acagaatcag cagcgaaccg cgatacggca
     5581 tgccgagaat aaacactgac accagcatac acagcaacat cgccagcatc ccgagaaagt
     5641 aataagggat cagcttacac agcagcagtt ccgtgcgggt aatctccgta gagagcagag
     5701 cctccatggt gccgcgttcc cattctcgcg ccaccaccag cgaggtgaga atcgcgccga
     5761 tgaccgtcat gataatggtc accgcacccg ggataataaa gtgctggctt atcgccgccg
     5821 ggttaaacca gtagcgggtt tgtacatcaa taagcggttc aaaagtctgc ccgttgtcct
     5881 ccgctcgctg catttgccag atctgccaga tcccttcgac atacccctgc acaaagttag
     5941 cggtattcgg ctcactgccg tcggtgatca cctgaatcgg tgcggtggcg ttggcgcgct
     6001 ccatctgttc ggcgaaatcc acgggaataa ccaccagacc gcgaattttc cccgactgca
     6061 ttttggcgat cagttcctga cggttatcgc tgatggtggc gtcgatgtag ggcgaaccgg
     6121 tcatggtgtg ggtgaaatcc agcgccgctt cgctacgttg ttccagcaaa atcccgaccc
     6181 gcagcttgct ggagtcgagg ttaatgccgt aaccaaaaat aaacagcagt agcagtggga
     6241 tcactaccgc aatcagccag ctactcggat cgcgaacgat ctgccgcgtc tctttgacgc
     6301 acagcgcccg cacgcgacgc caggaaagga tcgggttact cattgctatg ctccttatcc
     6361 cagtcgtgga tcaactgaat aaaggcttgc tccatggtgg gatcgggttg ctcatcgtta
     6421 gctgactgtg ctttcaaatc gtccggcgta ccgctggcga ttaatttccc acggtacacc
     6481 aggccgatgc ggtcgcaata ttccgcttca tccataaagt gggtggtgac catcaccgtg
     6541 acgcctttct ctaccatgct gttgatgtgc agccaaaatt cacggcgggt gagggggtca
     6601 acgccggaag tcggttcgtc gagaaagaga atatccggtt catgcatcag cgaacaggcc
     6661 agcgccagcc gctgtttaaa acctaatggc agttcatcgg tggcgtgtga ggcaatactt
     6721 ttcaggccaa atgcctcgct catacgggag attttttcgt tctgcgcccg accgcgtaag
     6781 ccatacacac cagagaaaaa gcgtaaattc tgttcgaccg tcaggttgcc gtagagcgaa
     6841 aatttttgcg ccatatagcc gagatgctgg cgcgctttac cggaactctc tttcagatcc
     6901 atccccagca ccagcgcctg gccggaagtc ggcaccagca aaccgcacat catcttaaag
     6961 gtggtcgatt tacccgcgcc gtttggcccc agcaaaccaa aaatctcccc acgtttaacg
     7021 gcaaagttga cgtgatcggt ggcggcaaaa tcaccaaatt tcttggtcag ttctttcgct
     7081 tcgatcaccg tctcgccggg tgtgccttct accgtatgca atattgcacc cagcggcgat
     7141 tctgaggttc cggcaccgcc cagcaaatca ataaacgcat cttcaaaacg cggcgtagtt
     7201 tcgttgatgt tgatttccgg catcccgtcg gcatggcgaa tatcgtctgg tgtggcctct
     7261 tttttgagga tcagacgtac cgatttcccc tgaatcatgc cgtcgctgac ctgcggcagt
     7321 ttcaaggcgc gttgcaacag tttgcggttg ccctcgtgtg gactggtcat cagaaagctg
     7381 cgtccggcca tggtttgtgt cagggctttt ggttctccct gatacagcaa ctcgccttcg
     7441 ttcatcagta acacgtcacg gcactgctcg gcttcgtcga gatacgaggt actccagagg
     7501 attaacatcc cttcgcccgc cagctcatgc accatctgcc acagttcgcg ccgtgagata
     7561 gggtcaacgc cgacgccggg ttcatcgagc agcaacactt tcggttcgcc caccagggta
     7621 caggccaggc cgagtttttg tttcatccca ccggagagct tgcccgccag gcgtccggta
     7681 aacggcccaa gagacgtaaa ctccagcagg cgagcaaaag tttgcttacg tgcctcgccg
     7741 gtgacgctgc gcaaatccgc gtacagattg aggttctcca tcaccgtgag atcttcatac
     7801 agaccaaatt tctgcggcat atagccgagc acggcgtgca gcgcgccgtc gtttttgatc
     7861 ggatcaaagc caatcaccgt ggcactgccg ctgtcgggtt tcagtaatcc cgccaacatc
     7921 cgcatcagcg tggttttacc tgcaccgtcc ggccccacca accccgtcac ataaccggcg
     7981 tgaatggtac aatcgagcgg cgcgacggcg ggcttgtcca tgcccggaaa gcgtttttcc
     8041 aggccgttca gcgtgataac ggcatcattc atgtcctgcc tcgtcaccga attgtaccgt
     8101 cattggcatt ccctggcgta acgcatcatc ggcgtcggtc accacaatac gcaggcgata
     8161 gacgaggtcg gtacgcagat ccggtgtttc gacggttttc ggggtaaatt cagcagtcgg
     8221 cgaaacgaaa ccaatctgcc cgtgatacgg cttgtccggg cgaccatcgg tataaagcag
     8281 cactttgcgc cccggctggg cctggtcaag attccgttca tcaacataag cgcgcaccca
     8341 caccggacgc gttagtgaaa cggtaaacac cgtgccacct tcattgagaa ccgtgcctgg
     8401 ctctaccgcg cgcgttaaca gcgtgccatc agacggtgct atcaacgttg agtcctgtaa
     8461 attcagctcc gcctgcgcca gttgcgcctg cgcctgttcg aggctggctt tcgcctgagc
     8521 gatgtcctgt tcacggttac cggaacggta ctgacgcaat ttatcctgtg ctgatttcag
     8581 cgttgcctgt gcctggtcgc gcgaggagcg ggcattttcc aggtcatttg ccgaaatggt
     8641 gcggcttttc cacagtcctt gctggcggtt atagaagttc tgcgcatagt cataggcggc
     8701 ttgcgcctgt ttcaccgctg cggcggcctg agcgatttct tcatcgcgat acccggcaag
     8761 catcagatca tactgcgcct gtgccaccga gacgcccgct ttcgcctgca tcagggcaat
     8821 ctcatacggc ttgtgatcca gttcgcccag cacctggccc gctttgatag catcaccttc
     8881 gtccaccgcc agcgattcaa cgcgcccccc aacacggaaa ctaagattca ccgtacgaat
     8941 atccacgttg ccatacagcg tcaggccgtt atcctggcgg ctttgatacc accagtagcc
     9001 tccggcaacc acggcggcaa gtaccactac cgccagtccg atcacgacag gttttttcat
     9061 cactacaaac tcctttgcga taatccttgc aaaatcaggt cgatatgaca ggtcaccgtc
     9121 tggttgatca gttcggtctt ttcttcatcg aacgcggtcc agccggtacg taacagaatt
     9181 gtttctttgc caagacggaa cgccagaatc tcaccaatca acgcatgggt atgaaggatc
     9241 atgcgggtgt cattagcgtc gcagccggtc caggcggcaa tcaggcgtgt caggtggctg
     9301 tgtagcggac taatcacctg ttcgtgcacc aggtggtagg ctgccgtggg agagagctgc
     9361 tcacgggaga taaacttgct gaggttgacg gtgtcatcct gggtgagcag cttaatcatg
     9421 ttcctgcagg cgcgaaggat cagttcacgg atggcagccc gatcaggctg tggttgggcg
     9481 aacaagcgtt cggcttcctc ggcatgcgga cggaactgct cgccaataaa atcggcaatc
     9541 cactgggcgc aggcgaggta caaatcttct ttcgaaccga agtagtaggt gatggcagcg
     9601 atattctgcc cggcctgggc ggctatctcg cgggtggtgg cgttcattcc atattcacca
     9661 aactgcgcca gtgcggcagc aatcagctgt tttttcgcct gttcaccctt gattgtcatg
     9721 gcaggattat tcatcgcaca gtccattctt aatcaaatga ttgattaaga ttatgactcc
     9781 atatgggagt tgtccagtat ggctaagaat tttagctacg ccagtcacag ggataattta
     9841 tgcgctgcgt cacaaaaact gctacactcc gctccctcat gacattgtgg tttttgtcat
     9901 tttccttttc agtatctccc tgaaaactac accggtaacg gtcggggcgg ttcggagtag
     9961 ttatgtcttt cgattctttg ggtttaagcc ctgatatcct gcgcgccgtt gccgagcagg
    10021 gttaccgtga acccacccct attcagcagc aggcgatccc tgcggtgctg gaaggccgcg
    10081 acctgatggc tagcgcccag accggcaccg gcaaaacagc gggctttacg ctgccgctgt
    10141 tgcaacacct gatcactcgc cagccgcacg ccaaagggcg tcgtccggta cgtgcgctca
    10201 ttcttacccc gacccgtgaa ctggcggcac agattggcga aaacgtccgt gattacagca
    10261 aatacctgaa cattcgttcg ctggtggtgt ttggcggtgt cagcattaac ccacaaatga
    10321 tgaagttgcg cggcggcgtt gatgtgctgg tggcaacgcc gggacgtttg ctggacctgg
    10381 aacatcaaaa tgcagtgaag ctggatcagg ttgaaatcct cgtcctcgat gaagctgacc
    10441 gcatgctcga catgggcttt atccacgata tccgtcgcgt gttaacaaaa ctgccagcga
    10501 aacgccagaa cctgctgttc tctgcgacct tctctgacga tattaaagcc ctggcggaaa
    10561 aactgttgca caacccgctg gaaatcgaag tggcacgccg caataccgcg tctgatcagg
    10621 tgacccagca cgttcacttt gtcgataaga aacgcaaacg cgaattgctg tcgcacatga
    10681 ttggtaaagg gaactggcag caggtgctgg tgtttacccg taccaaacac ggcgctaacc
    10741 atctggctga gcagctcaat aaagatggca tccgtagtgc ggcgatccac gggaataaat
    10801 cgcaaggtgc gcgtacccgt gcgctggctg attttaaatc gggtgacatt cgcgtactgg
    10861 tggcgaccga tatcgttgcg cgcggtctgg atatcgaaga gttgccgcac gtcgtcaact
    10921 atgaactgcc aaacgtaccg gaagattatg tccaccgtat cgggcgtacc ggtcgtgcgg
    10981 ctgctaccgg tgaagcgttg tcgctggtgt gtgttgatga acacaaactg ctgcgtgata
    11041 tcgaaaaact gctgaaaaaa gagatcccgc gcattgcgat tccgggctat gagccggacc
    11101 cgtcaatcaa agccgaaccg atccagaacg gtcgccagca acgtggcggc ggcggtcgtg
    11161 ggcaaggtgg tggtcgcggt caacagcaac cacgccgtgg ggaaggtggc gcaaaatctg
    11221 caagcgcgaa acctgcagaa aaaccgtctc gccgcctcgg cgatgccaaa ccggcaggcg
    11281 aacaacaacg tcgccgccgt ccgcgtaaac ctgccgctgc gcagtaatct tttatgccgg
    11341 gctatgcccg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc
    11401 aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg
    11461 tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa
    11521 aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt
    11581 tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct
    11641 ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag
    11701 agcacacgca gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt
    11761 tctttaaccg actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg
    11821 gcgaccatgg gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt
    11881 tgtgcctgaa aatagtgttc ggaggtgggc taggtattcc cgtcaacctt gatgggccag
    11941 gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg
    12001 tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt
    12061 ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg
    12121 ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa ccgtcgcgct
    12181 taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc
    12241 ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg
    12301 gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc
    12361 cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc
    12421 gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct
    12481 taaattcact gccgctttcg ggcgtgggcg ctacgtttgt ccgcgtaatc tgacggcgct
    12541 cgccagcact gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc
    12601 aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa
    12661 atgggatggt ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt
    12721 gagtaccgac aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt
    12781 ttttgttact cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt
    12841 gatggcggcg atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct
    12901 ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat
    12961 caccgcgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat
    13021 ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca
    13081 ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc
    13141 tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga
    13201 gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct
    13261 taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt
    13321 acagatgaac cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc
    13381 tctggcgcaa tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg
    13441 gcagctacac ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct
    13501 gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag
    13561 tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc
    13621 gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt
    13681 tgagccttcc atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga
    13741 acaggtggag agcaaaaaac atctcggtat gttggtactg tttgccagcg gacgggcgat
    13801 gcagcgcttt ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca
    13861 gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt
    13921 gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca
    13981 ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg
    14041 ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc
    14101 gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt
    14161 ggttatctac gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt
    14221 accggtattt ccgatagagc aaccggaagt ccctgaaggt atagctaaaa agaaagaaaa
    14281 aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat
    14341 ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga
    14401 ccgggatact gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga
    14461 gttgggcggc gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg
    14521 cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat
    14581 gccgattgtc atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt
    14641 ggcgattctg ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc
    14701 aactcgcgtg ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg
    14761 cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt
    14821 ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc
    14881 acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcactgc gtctttccag
    14941 cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg
    15001 ggcgttacta atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca
    15061 gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg
    15121 tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg
    15181 cctggctggc agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt
    15241 ggctacgggt gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcattttg
    15301 atttttatct cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat
    15361 tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca
    15421 taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac
    15481 agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt
    15541 gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc
    15601 ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc
    15661 gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg
    15721 gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg
    15781 gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg
    15841 gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc
    15901 cacggtcgcg acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat
    15961 aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc
    16021 gcctggcata aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg
    16081 acgaccaatc cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa
    16141 cataaaggct acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt
    16201 aaaacgacgc atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc
    16261 actatcatca tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc
    16321 tttgccgagt gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc
    16381 gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga
    16441 agctggcagg ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag
    16501 gctttctgtc agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca
    16561 agggtaacat aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga
    16621 aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg
    16681 cctgataaga cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct
    16741 tatccgtcct acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt
    16801 ggcggaa
//
LOCUS       DADLSB010000086        16606 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.086, whole genome shotgun sequence.
ACCESSION   DADLSB010000086 DADLSB010000000
VERSION     DADLSB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.086"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1868)
                     /gene="yeaG"
                     /locus_tag="JN331_002598"
     CDS             complement(<1..1868)
                     /gene="yeaG"
                     /locus_tag="JN331_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY7260716.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMME"
     gene            2304..3050
                     /locus_tag="JN331_002599"
     CDS             2304..3050
                     /locus_tag="JN331_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY7260717.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3140..3901
                     /locus_tag="JN331_002600"
     CDS             3140..3901
                     /locus_tag="JN331_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY7260718.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3952..4836)
                     /locus_tag="JN331_002601"
     CDS             complement(3952..4836)
                     /locus_tag="JN331_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY7260719.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4920..5915)
                     /gene="gapA"
                     /locus_tag="JN331_002602"
     CDS             complement(4920..5915)
                     /gene="gapA"
                     /locus_tag="JN331_002602"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY7260720.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            6257..6670
                     /gene="msrB"
                     /locus_tag="JN331_002603"
     CDS             6257..6670
                     /gene="msrB"
                     /locus_tag="JN331_002603"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY7260721.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6712..6984
                     /locus_tag="JN331_002604"
     CDS             6712..6984
                     /locus_tag="JN331_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY7260722.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            7354..8430
                     /locus_tag="JN331_002605"
     CDS             7354..8430
                     /locus_tag="JN331_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7260723.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSSANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            8457..9836
                     /locus_tag="JN331_002606"
     CDS             8457..9836
                     /locus_tag="JN331_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7260724.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLLAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9853..10896
                     /locus_tag="JN331_002607"
     CDS             9853..10896
                     /locus_tag="JN331_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HAY7260725.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            10917..11753
                     /locus_tag="JN331_002608"
     CDS             10917..11753
                     /locus_tag="JN331_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY7260726.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11758..12705
                     /locus_tag="JN331_002609"
     CDS             11758..12705
                     /locus_tag="JN331_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY7260727.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12715..13695
                     /gene="ydjG"
                     /locus_tag="JN331_002610"
     CDS             12715..13695
                     /gene="ydjG"
                     /locus_tag="JN331_002610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY7260728.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKLIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13832..14590
                     /locus_tag="JN331_002611"
     CDS             13832..14590
                     /locus_tag="JN331_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7260729.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14708..16066
                     /locus_tag="JN331_002612"
     CDS             14708..16066
                     /locus_tag="JN331_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7260730.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(16159..>16606)
                     /gene="pncA"
                     /locus_tag="JN331_002613"
     CDS             complement(16159..>16606)
                     /gene="pncA"
                     /locus_tag="JN331_002613"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY7260731.1"
                     /translation="GVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQKEIAAVF
                     HKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFTVLDALQL
                     GYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
BASE COUNT         4261 a         3692 c         4201 g         4452 t
ORIGIN      
        1 ttttccatca tacggtcgac aaagtcgtcg tgtttcttct gctcgtcggt tgacgttttg
       61 gcgttaaacg agataaccgg caacagctcc tcggtattgg agaacatttt cttctcgata
      121 accgtgcgca gtttttcata gctggtccag ttcggattgc gtccgctgtt attcgctctg
      181 gcgcgcagta cgaagttaac aatctcgttg cggaaatctt ttggattact gatccccgcc
      241 ggtttctcga ttttctccag ctcggcgtta agagactcgc ggtcaaacag ctgcccggta
      301 tccggatcgc gatactcctg atcctgaatc cagaaatccg cgtaggtaac ataacggtcg
      361 aaaatgtttt gcccatattc ggaataggat tcaaggtagg ccgtctggat ctctttgccg
      421 ataaactcgg catatttcgg gatcagataa cctttcagga actccagata gcgttctgcc
      481 tgctcttgcg ggaactgctc gcgctcgatc tgctgttcca ggacgtagaa cagatggacc
      541 gggtttgccg ccacttctac atgatcgaag ttgaacacgc gggagaggat cttaaacgca
      601 aaacgcgtcg acagaccgtt catcccttca tcgacaccgg cgtagtcacg atattcctga
      661 tacgacttgg ctttcggatc ggtgtctttc agactttcgc catcataaac ccgcatcttt
      721 gaataaatgc tggagttttc tggctctttc aggcgcgaaa gaatggaaaa acgtgacagt
      781 gtttcgagcg tgccaggggc gcatggggcg tgagtcaatt cactgtgatt aagcaatttc
      841 tcgtagattt tgatctcttc ggaaatgcgc aagcaatacg gcaccttcac gatgtaaaca
      901 cggtcgagga aggcttcgtt gtttttgtta ttacggaaag tgacccattc ggactcgttc
      961 gagtgggcga gaataatccc gttgaacggc agggcggaga taccttccgt cccgttgtag
     1021 ttaccttcct gagtggcggt taacaaggga tgcagcactt ttatcggtgc tttaaacatc
     1081 tcaacgaatt ccatgatccc ctgattggcg cggcacagcg caccggaata gccgtaggcg
     1141 tccgggtcat tctgcgcgta gtgttcgagt ttacgaatat cgactttccc aaccagcgcg
     1201 gagatgtcct ggttgttctc atcaccgggt tccgttttgg cgatagcaat ttgttgcaga
     1261 attgacggcc agaccttcac tacccggaac ttagtgatat cgccaccaaa ttcatgcagg
     1321 cgttttgccg cccatggcga catgatggtg ccgagataac ggcgaggaat gccatactct
     1381 ttttccagaa tctgagcatc ttcctgcgga ttgaaaagac agaacggatg atcgttgacc
     1441 gggctacgct caccgttggc gctcaataca taaatcggta cgagctgcat taatgatttc
     1501 agtcgctcag caagcgatga tttaccccca cccacaggcc ccagcagata caggatttgt
     1561 ttcttctctt ccagcccctg agccgcgtgt ttcagataag agacaatctg ttcaatcgcg
     1621 tcttccatgc cgtaaaactc ttcaaatgcc ggataacgtg caatgacccg gttagaaaag
     1681 agtcgagaaa gtctgggttc ctgggctgta tcgaccatga caggctcacc gatagccatc
     1741 aatagccgct cagccgcgtt ggcataagca ctgcgatctt gccgacaagt ggtaagaaac
     1801 tcctgcagtg tgaactcttc gtccttggca gcttcatatc gctggcgata gtgatcgaat
     1861 atattcatgg tatgccgtcc tttcgttttt tagcacaggt taagagccgt tcatatgaat
     1921 agtagaggct cccggaagag gtaaactgaa cgacgtctct tgctaaaaca cagcaactct
     1981 catgccattt tatcgacgat gagaacaacg tgcccggtga tacaccttca aaattaagcg
     2041 tagatggcaa atggaaaact tgcctgcacg gctttcttaa tttcaatggc atatcaacaa
     2101 ctcatccata aattttgcat aattaatgta aagaccaggc tcgccagtaa cgctaaattc
     2161 atttggctgt aagcgcggtg tcatccgcgg caggaaaatt aaacagttac tttaaaaaat
     2221 gaaaacgtaa aaaggttggg tttcgatgta ttgacgggta aactttgtcg cccgctaaac
     2281 atttgtttaa ggaatgatta attgtgacca aactcaaact tctggcactt ggagtgctta
     2341 tcgcaacgtc tgcaggcgta gcgcacgctg aaggtaaatt ttccctgggc gcaggcgtag
     2401 gtgtcgttga gcacccatat aaagattacg ataccgatgt ttacccagta ccggtaatca
     2461 actatgaagg cgataacttc tggttccgtg gcttaggtgg tggttactac ctgtggaatg
     2521 acgcaacgga taaactttca attaccgctt actggtcgcc gctttacttc aaagcgaaag
     2581 acagtggcga tcaccaaatg cgtcacctgg atgaccgtaa gagcaccatg atggctggtc
     2641 tgtcttatgc tcactttacc cagtacggtt acctgcgtac caccctggct ggcgataccc
     2701 tggataacag caacggcatt gtctgggata tggcctggtt gtatcgttac accaacggtg
     2761 gcctgaccgt gactccgggt attggtgtgc agtggaacag cgaaaaccag aacgaatact
     2821 attatggcgt atcgcgcaaa gagtccgctc gcagcggtct gcgtggctat aacccgaatg
     2881 acagctggag cccttacctt gagctgagcg ccagctacaa cttcctcggc gactggagtg
     2941 tttacggtac cgcgcgctac acccgtctgt ctgatgaagt tactgacagc ccgatggtgg
     3001 ataaatcctg gactggcctg atttctaccg ggatcaccta caaattctga taatgcatct
     3061 ttccagggca ttttgtgcat aaaaacaggg cgtcacgccc tgttttgcat tacggtggtg
     3121 caatacctgg gaggaaacaa tgcaacaaaa aatgattcaa tttagtggcg atgtctcact
     3181 gccagccata gggcagggaa catggtatat gggcgaagat gccagtcagc gcaaaacaga
     3241 agttgctgca ctacgcgcag gcattgaact cggtttaacc ctcattgata ccgccgaaat
     3301 gtatgccgat ggcggtgccg aaaaggtggt tggggaagca ttaaccggtc tgcgagagaa
     3361 ggtctttctc gtctctaaag tctatccgtg gaatgctggc gggcaaaaag cgataaatgc
     3421 atgcgaagcc agtttacgcc gtctcaatac tgattatctc gatctctact tattacactg
     3481 gtctggcagt ttcgcttttg aagagactgt tgctgcgatg gaaaaattga tcgcccaggg
     3541 aaaaatccgt cgctggggcg tttctaacct tggttatgcc gatatgcagg aactctggca
     3601 gctgccgggg ggaaatcagt gtgccactaa tcaggtgctt taccatctcg gttcacgagg
     3661 gattgagtac gatctactcc cctggtgcca gcaacagcag atgccggtga tggcttacag
     3721 tccattagcc caggccgggc ggttgcgcaa tggactgtta aaaaacgcgg tagtcaacga
     3781 aattgcacat gtccaacaaa atgcagctgc gcttgaggtc gaactttcgt cagcggaatt
     3841 agctatgctg gataaggcat atccggcacc aaaaggaaaa actgcgctgg atatggtgtg
     3901 atgttgatac gtgccggatg catcgcaccc ggcacacaac attaacgcaa attaacgttt
     3961 ggcaacgcga atggattgcg ccagatgtgc aggtttctct ttggtgactt tttgcgtttc
     4021 tgaagcgtaa gccgtttcta cacaaacaaa ggtcttgtag ccatcgtccg gcatgtcgcc
     4081 catgctaact gaaagcgccg gtcccgggtt ccagccgaca acgttcagat gatgctggtg
     4141 gcctacggcg ataatacgat tcagcgcttc atcattaatc acgctgcaat cttgcggatt
     4201 cagatacacg cgatcggtac ggtcagggaa ggtctgaata ccgtcggtca gtacatcttc
     4261 tttcgcgtca ttcactttat caatgaagcg atcgcccagc ccactgacgc ttaccttagc
     4321 gatatcaccc acgttaaagt aggtatgcag ggcagaggtg gtttcaaatt cgccatgtga
     4381 ttcaagatcg atttcacagg ttttacccac gcggaaatgc gctaacagcg taaagtcgtg
     4441 cggccagaat tttttcgtct cttcgctttg tgtcaattca aaagtcagcg ctacgccatc
     4501 agcatcttca tgatgtgatt tcagcgtcca cggcaggttg cgggcaaaac cgtgcgcagg
     4561 cagaccttgt tgtgccgccg gaccaaacca cggccagcaa accggtacgc caccgcgaat
     4621 agcgacgcca tttttgaacg gtgtgttgtt gctcaaccac agaacttctt cttcacccgc
     4681 aggtttccac gagagaaggt gtgcgccctg taatgcaaaa gaggctttta cctggggatg
     4741 atcgaccaca atgaggtcca gttcatccag tttacgacgg gagaggacag gggagatttg
     4801 ttcgatgacc ggaagggcaa aaattttctt aatcatgatg cagtccttta acttcatttt
     4861 atcaggtaaa aaaaagagcg accgaagtcg ctctttttag atcacagtgt catctcaact
     4921 tatttggaga tgtgagcgat caggtccaga actttgttgg agtaaccggt ttcgttgtcg
     4981 taccaggata ccagtttcac gaagttgtcg ttcagagcga taccagcttt agcatcgaac
     5041 acggaagtgc aaacttcgcc gttgaaatcg gtagatacta cgtcatcttc ggtgtagccc
     5101 agaacgcctt tcatttcgcc ttcagcagca gctttaacgg cagctttgat ctgctcgtaa
     5161 gttgcagctt tttccagacg aacggtcagg tcaactacgg atacgttcgg ggtcggaacg
     5221 cggaacgcca taccagtcag tttgccattc agttctggca gtactttacc tacagcttta
     5281 gcagcaccgg tagaggacgg gatgatgttc tgggaagcgc cgcggccgcc gcgccagtct
     5341 ttgtgagacg ggccatcaac ggttttctga gtagcggtag tagcgtgaac ggtggtcatc
     5401 agaccttcga tgatgccgaa gttatcgttg ataactttag ccagcggagc caggcagttg
     5461 gtggtgcagg aagcgttgga aacgatgtcc tggccagcat atttgtcgaa gttagcgcct
     5521 ttaacgaaca tcggagtgtt gtctttagac ggaccagtca taaccacttt cttcgcacca
     5581 gcggtgatgt gtttacgagc agtttcgtca gtcaggaaca gaccagttgc ttcagcgaca
     5641 acgtcaacac ccacttcgtc ccatttcagg ttagccggat cacgttcagc ggtaacacgg
     5701 atttttttac cgttaacgat cagatgaccg tctttcactt caacggtacc gtcgaaacgg
     5761 ccgtgagtgg agtcatattt cagcatgtat gccatgtaat cagcgtctaa caggtcgttg
     5821 attgcaacga tctcgatgtc agaacgtttc tgagcagcac ggaaaacaat gcgaccgata
     5881 cggccaaaac cgttgatacc tactttgata gtcatatatt ccaccagcta tttgttagtg
     5941 aataaaaggt tgcctgtaaa attacaaaaa ccttacgcag cgtcaagcgg aatcgtgtca
     6001 atcattgcga caaatcaatc ctgtgcctaa gcattacgcg actgactcgc ctcactcttc
     6061 ctttgggctt gagaccacat ggggtcggcg ccccgaattt taaagggcaa ttacgataaa
     6121 aatgtgattt tcatcacgat ttaggtgcag ccatttcgtc gagatcaagt ttaggacaaa
     6181 attttgccag ttggtgttaa tgttttgtta gaatcagtca cgtaatgtga gcacgattaa
     6241 agtgagatgt gagtaaatgg ctaataaacc ttcggcagaa gaactgaaaa aaaatttgtc
     6301 cgagatgcag ttttacgtga cgcagaatca tgggacagaa ccgccattta cgggtcgttt
     6361 actgcataac aagcgtgacg gcgtatatca ctgtttgatc tgcgatgccc cgctgtttca
     6421 ttccgaaacc aagtatgatt ccggctgtgg ctggcccagt ttctacgaac cggtgagtga
     6481 agaatccatt cgttatatca aagacttgtc acatggaatg cagcgcatag aaattcgttg
     6541 cggtaactgt gatgcccatc tggggcatgt ctttcccgac gggccgcaac caacaggcga
     6601 acgttattgt gttaactctg cttctttacg cttcactgat ggcgaaaacg gcgaagaaat
     6661 caacggttga aaaaacgatt cagcaactta ttccacagga gcggaaaaat tatgaatctt
     6721 gatgacatta tcaacagcat gacgcctgag gtataccagc gtttgtcgac cgccgttgaa
     6781 ctggggaaat ggcctgatgg cgttgcgttg accgaggaac aaaaagagaa ttgcctgcaa
     6841 ctggtgatgc tgtggcaagc ccgccataat accgaagcac agcatatgac aattgacaca
     6901 aatggtcaga tggtcatgaa gagcaaacag cagttaaaag aagattttgg tatctcagca
     6961 aagcctattg ccatgtttaa gtaagtggtt catccccttt ttgccgcggc tatgaagggg
     7021 atggccaaca atgaccccac gactcgtctc aattatctcg acccgacctg gctaagtccc
     7081 agtccgatct cttgcagtcg cgttcacgcc tgaatgactt ctcctgaact taagtctcag
     7141 acctatttgg ccggtaatcc ctctcgaatt tgctcttttt gcgtcccgcc ttccaccaac
     7201 ctgtaacaga cccagccatt ttctgcgtaa ttgtgatcta agttgtaaag cgcaggtcat
     7261 tatctgtgtt atgcagattt aatgttgacc tcaaaaatca tgtgggtaag tatgatggcc
     7321 atctaagcaa aacaaaacac agaggatgac gcaatgaaag cactggctcg gtttggcaag
     7381 gcctttggcg gctacaagat gattcatgtc ccacaaccca tttgtggccc ggaagacgta
     7441 gtgattgaaa ttaaagccgc ggcaatctgc ggcgcagaca tgaagcacta caatgtcgat
     7501 agcggttctg atgagtttaa ctctatccgc ggccatgagt tcgcaggttg tattgcgcag
     7561 gttggtgaaa aagtcaaaga ctggaaagtg gggcaacgcg tcgtgtcgga taacagcggt
     7621 cacgtttgcg gcgtttgtcc ggcctgtgaa cagggtgatt ttctgtgttg tacagaaaaa
     7681 gtaaaccttg gtctggataa caatacctgg ggcggtggtt tttccaaata ttgtctggtt
     7741 cctggtgaaa ttctcaaaat tcatcgtcat gcgttgtggg aaatccctga tggtgttgat
     7801 tatgaggacg cagccgtact tgaccctatc tgtaatgcct ataaatccat cgcgcagcaa
     7861 tcgaaattcc ttcctggtca ggatgttgtc gtcattggca ctggcccact cgggctgttt
     7921 tccgtacaaa tggcgcggat tatgggggcg gtaaatatcg tcgtcgttgg tctgcaagaa
     7981 gatgtggcgg tccgcttccc ggttgcaaaa gaactggggg cgacggcagt agtaaatggt
     8041 tctaccgaag atgtggtggc gcgctgccag caaatttgtg gcaaagataa tctgggactg
     8101 gtgattgaat gctccagtgc caatatcgca ctgaaacaag ccatcgaaat gctccgcccg
     8161 aatggggaag tggtacgcgt tggaatgggc ttcaaacctc ttgatttctc gattaatgac
     8221 attaccgcct ggaataaaag catcattggg catatggcct atgactccac ctcatggcgt
     8281 aacgctatcc ggctattagc cagcggcgct atcaaagtca aaccgatgat cacgcatcgt
     8341 atcggcctgt cgcaatggcg cgaagggttt gatgcgatgg tcgataaaac cgcaatcaaa
     8401 gtgatcatga cttacgactt tgatgaataa ctactctcct gaaggaacgt tatcgcatgg
     8461 aacagataac aaaaccgcat tgtggtgcac ggctggatcg cttaccggat tgccgctggc
     8521 attcgtcaat gtttgccatc gtcgcgtttg gcttgcttgt ctgctggagt aatgccgttg
     8581 gtggcttgat cctcgcgcag ctgaaagcgt tgggctggac agataattcc accacagcca
     8641 cattctcagc aatcacgacc gccggaatgt ttctcggtgc gctggtgggc ggcatcattg
     8701 gcgacaaaac cggtcgcaga aatgcgttca tcctctatga ggccattcat attgcctcga
     8761 tggttgtcgg tgctttctca ccgaatatgg attttcttat tgcctgccgt tttgtgatgg
     8821 gcgttgggct gggagcttta ctggttacac tgcttgctgg tttcaccgaa tatatgcccg
     8881 gtagaaatcg cggaacgtgg tccagtcgtg tttcctttat tggcaactgg tcatatccgc
     8941 tctgctcact gattgcgatg ggactcacgc cgctgattag tgcagagtgg aactggcggg
     9001 tacaactgct tattcctgca atattgtcgc ttatcgctac ggcgctggcc tggcgctact
     9061 ttcctgaatc cccgcgctgg ctggaatcgc gcggacggta tcaggaagcc gagaaagtca
     9121 tgcggagtat agaagaaggc gtcatacgcc agacgggtaa acctttgccg cctgtggtta
     9181 ttgctgatga cggtaaagcg ccacaagcgg tgccgtattc agccttactg acaggagtat
     9241 tactgaaacg cgtgatatta ggctcttgtg tgctgattac catgaacgtt gtgcagtaca
     9301 cactaattaa ctggttgcca acaatattca tgacccaggg gattaattta aaagactcga
     9361 ttgttttaaa taccatgagt atgtttggcg cgccatttgg tatttttatt gccatgctgg
     9421 tgatggataa aattccgcgt aaaacaatgg gtgtggggct attaatcctg attgcggtgc
     9481 tcggatatat ctattcactg caaaccagta tgttgctcat aacgctgatt ggtttcttcc
     9541 tgattacttt cgtctatatg tacgtttgct atgcctcggc agtgtatgtc cctgaaatct
     9601 ggccgacaga ggccaaactc cgtggctccg gtctggcgaa tgcggtaggg cgaatcagtg
     9661 gtattgccgc accttatgcc gttgcagtgc tgctcagtag ttatggtgta acgggagtct
     9721 ttattcttct gggggctgtt tcaattattg tcgcaattgc tatcgccacc attggaattg
     9781 aaaccaaagg tgtctccgtt gaaagtttaa gtattgatgc agtagccaat aaataatacg
     9841 ggtggattca aaatgaaaaa ttcaaaagca atattgcagg tgccgggcac aatgaaaatt
     9901 atttcagcag aaataccagt gcctaaagaa gatgaagttt tgattaaagt agaatatgtc
     9961 ggtatttgtg gttcagatgt acatggtttt gaatcaggcc cgtttattcc gcctaaagac
    10021 ccaaatcaag aaattggcct gggtcatgaa tgcgccggga cggttgtggc tgtgggaagc
    10081 cgcgtgcgca aatttaaacc gggggatcgg gtaaatatcg aacctggcgt tccttgcggt
    10141 cactgtcgtt actgtctgga aggcaaatat aacatctgcc cggacgttga ttttatggcg
    10201 acacaaccca actaccgcgg cgcattaacg cactatctgt gtcatccgga gagctttact
    10261 tacaaactgc ccgacaatat ggacacgatg gaaggggcgc tggtggagcc tgccgcagtc
    10321 gggatgcatg ccgcgatgct ggcagatgtt aaaccgggta agaagataat tattctggga
    10381 gcaggttgta ttggtttgat gacgttgcaa gcgtgcaaat gcctgggcgc aacggaaatt
    10441 gccgtcgttg atgtactgga aaaacgtctg gcaatggcgg aacagcttgg tgcgacagtg
    10501 gttattaatg gcgcaaaaga ggacactatt gcacgctgtc agcaatttac cgaagacatg
    10561 ggcgcagata ttgttttcga aacagcgggt tctgcggtca ccgttaaaca ggcaccttat
    10621 ctggtaatgc gcggcggtaa aattatgatt gttggtactg tacccggcga ttcggcaatc
    10681 aatttcctca aaatcaatcg cgaagtcact atccagacgg tattccgcta tgccaatcgt
    10741 tatccggtca cgattgaagc tatttcttca gggcgattcg atgtgaaatc gatggtgacg
    10801 catatttacg attatcggga tgtacaacag gcatttgaag agtcagttaa caacaaacgc
    10861 gacattatta aaggcgttat taaaattagc gattaattaa tacgggagaa cagaacatgc
    10921 tcgcagatat cagatattgg gaaaacgatg ccacaaataa acattatgca attgcccatt
    10981 ttaacgtatg gaatgcagaa atgttgatgg gcgttaaaga cgcagccgaa gaagcgaaat
    11041 ccccggttat tatttctttt ggtacaggtt ttgtcggtaa cacctcattt gaagatttct
    11101 ctcacatgat ggtatcaatg gcacaaaaag caacggtgcc ggtaataact cattgggatc
    11161 atggtcggag tatggagatt attcataacg cctggactca tggtatgaat tcattaatgc
    11221 gtgatgcttc tgcatttgat ttcgaagaaa atattcgttt aaccaaagag gctgttgatt
    11281 tctttcatcc gctgggtatt ccggtagagg cggaattagg gcatgtcggt aatgaaaccg
    11341 tttatgagga agcgctggcg ggctatcact ataccgatcc tgaccaggcg gctgaatttg
    11401 ttgaacgtac gggctgtgac tcattggctg tcgccatcgg caaccagcat ggggtttata
    11461 cgtcagagcc gcaattgaac tttgaggtcg tcaaacgcgt acgcgatgct gtttctgttc
    11521 cgctggtttt gcatggcgca tcggggatca gtgatgccga cattaaaact gcaatttcgt
    11581 tgggtattgc gaaaatcaac attcatacgg agctctgtca ggccgcaatg gtagcagtga
    11641 aagaaaacca ggatcagcct ttcctgcatt tagaacgtga agtacgtaaa gctgtaaaag
    11701 aacgggcatt ggaaaaaatt gaactgttcg gttctgatgg caaagcggaa taatgatatg
    11761 gataatctcg acgttatttg tataggtgcc gctattgttg atattccatt acaaccggtc
    11821 agtaaaaata tctttgatgt agattcttat cctcttgaaa gaatcgcaat gagcaccggg
    11881 ggcgatgcaa taaatgaagc aacaattatt tctcgtctgg ggcatcgtac cgcattaatg
    11941 agccgcattg gtaaagatgc ctcagggcaa tttattctcg accattgtcg caaagagaat
    12001 attgatattc aaagcctgaa acaggacgtc agcatagata catctattaa cgttggactg
    12061 gtgacggagg atggcgagcg gacgtttgtc acgaaccgta acggcagttt gtggaaatta
    12121 aatattgacg atgttgattt tgcgcggttt cctcaggcga aattattatc actggccagt
    12181 attttcaaca gtcctctatt ggatggtaaa gcgctaacag aaatttttac gcaagcgaaa
    12241 gcccggcaga tgatcatttg tgccgatatg atcaaaccgc ggttgaatga aacgctggat
    12301 gatatttgcg aagcattgag ttatgtcgat tatctgtttc caaattttgc cgaggcaaaa
    12361 ttactcacag ggaaagagac actggatgaa attgctgact gctttcttgc gtgcggcgta
    12421 aaaacggtgg tgattaaaac gggtaaagac ggctgcttta tcaagcgtgg tgacatgacg
    12481 atgaaggtgc cggcggtcgc aggaataacc gccatcgaca ccattggcgc gggcgataac
    12541 tttgcttcag gttttattgc ggcactgtta gaaggcaaaa atctgcgtga atgcgcacgc
    12601 tttgccaatg caacggcggc tatctcggtt ctaagcgtcg gtgccaccac cggcgtaaaa
    12661 aacagaaagc tggtcgaaca attgctggaa gaatacgaag gataatgaag gcaaatgaaa
    12721 aagatacctt taggcacaac ggatataacg ctttcgcgaa tggggttggg gacatgggcc
    12781 attggcggcg gtcctgcatg gaatggcgat ctcgatcggc aaatatgtat tgatacgatt
    12841 cttgaagccc atcgttgtgg cattaatctg attgatactg cgccaggata taactttggc
    12901 aatagtgaag ttatcgtcgg tcaggcgtta aaaaaactgc cccgtgaaca ggttgtagta
    12961 gaaaccaaat gcggcattgt ctgggaacga aaaggaagtt tattcaacaa agttggcgat
    13021 cggcagttgt ataaaaacct ttccccggaa tctatccgcg aagaggtaga agccagcttg
    13081 caacgtctgg gtattgatta catcgatatc tatatgacgc actggcagtc ggtgccgcca
    13141 ttttttacgc cgatagctga aactgtcgca gtgcttaatg agttaaaggc tgaagggaaa
    13201 attcgcgcta taggcgctgc taacgtcgat gctgaccata tccgcgagta tctgcaatat
    13261 ggtgaactgg atattattca ggcgaaatac agtattctcg accgggcaat ggaaaacgaa
    13321 ctgctgccgc tatgtcgtga taatggcatt gtggttcagg tttattcccc gctagagcag
    13381 ggattgttga ccggcaccat cactcgtgat tacgttccgg gcggcgctcg ggcaaataaa
    13441 gtctggttcc agcgtgaaaa catgctgaaa ttgattgata tgcttgaaca gtggcagcca
    13501 ctttgtgctc gttatcagtg cacaattccc actctggcac tggcgtggat attaaaacag
    13561 agtgatttaa tctccattct tagtggggct actgcaccgg aacaggtacg cgaaaatgtc
    13621 gcggcactga atatcaactt atcggatgca gacgcaacat tgatgaggga aatggcagag
    13681 gccctggagc gttaaatact caacacgatt cacctctgtt tctgtgaaaa ctgtgatgaa
    13741 aatctgtgat tcaaataggt tattttgaaa gtaaacatcg gttatgcgat aatcgcgcta
    13801 atgtgatgta aaaagataca ggggtgtaat cgtggcggca aaagacagga ttcaggcaat
    13861 taagcaaatg gttgccaacg ataaaaaggt gacagtctca aatttgagtg ggatttttca
    13921 ggtaaccgaa gaaaccattc gccgcgatct tgagaagctg gaagatgaag gctttttgac
    13981 cagaacctat ggtggtgctg ttttaaatac agcgatgttg acggagaata tccattttta
    14041 taagcgcgct tcatcgtttt atgaagagaa gcagcttatt gcacgcaagg cactaccctt
    14101 tatcgacaat aaaaccacca tggcagccga ttccagtagt accgtaatgg aattgctcaa
    14161 attgttacag gaccgtagtg acctgacgtt gctaaccaac tcggcggaag cgattcatgt
    14221 tctggctcag tcagaaatta aagtcgtttc aacgggtggg gaactaaaca aaaatacact
    14281 ttccctgcaa ggaagaatta ctaaagagat catcagccgc taccatgtcg atatcatggt
    14341 aatgagttgt aaaggtcttg atattaacag cggcgcgctg gactctaatg aagcggaagc
    14401 tgaaatcaaa aagacaatga tccgtcaggc gacagaagtt gcgttattgg ttgatcactc
    14461 taagtttgat cgtaaagctt ttgtccagtt agctgacttt agtcatatta attatataat
    14521 aactgataaa tcaccgggtg cagagtggat agcattttgc aaagacaata atatccagct
    14581 ggtgtggtaa tttaagcaca cgttgtcaat attatttgtt cctgcgcctg gaaataaaat
    14641 gatgataaag atgcggcaag tgaatttgcc gcaattgcta ccttttttag caaatccgag
    14701 gcacccaatg gaacaatatg atcaaattgg cgcaagactg gaccgcttgc ctttggcccg
    14761 gtttcattat cgtatatttg gtataataag ctttagtctg ttattaacgg ggtttttgag
    14821 ttactcaggt aatgtcgtct tagcaaagct ggtaagcaat ggatggtcaa ataatttcct
    14881 caatgccgcc tttacctcgg cattaatgtt tggttatttc atcggctcac ttactggtgg
    14941 gtttattggt gactactttg ggcggcgcag ggcgtttcgc ataaatcttc tcatcgtcgg
    15001 tattgctgca acaggggccg cttttgtccc tgatatgtac tggctcattt tctttcgctt
    15061 cctgatggga acaggaatgg gggcgctgat tatggttggc tatgcctcat ttacggagtt
    15121 tatccccgcg acggtgcgtg gaaaatggtc cgcgcggctc tcatttgttg gtaactggtc
    15181 gcccatgctg tctgcggcga taggcgtggt ggttatcgct ttttttagtt ggcgaataat
    15241 gtttcttctg ggtggtattg gcatactgtt agcctggttt ctctcaggta aatactttat
    15301 cgagtcgcca cgatggctgg cagggaaagg gcaaatcgcc ggtgcagaaa gccaacttcg
    15361 tgaagtagag cagcaaattg aaagggagaa gagtattcgt ttaccccagc ttactttgaa
    15421 ccagagcaac agcaaggtta aggtaatcaa gggtaccttc tggctcctgt ttaaagggga
    15481 aatgttacga cgtacattag ttgcgataac tgttttaatt gcaatgaaca tttcgcttta
    15541 taccattacc gtatggatac cgaccatatt tgttaactcc ggcattgatg tcgataaatc
    15601 aatattaatg accgctgtta ttatgattgg tgctccggta ggaatattta ttgcggcatt
    15661 aattattgat cattttcctc gtcgattatt tggctccgcc ttacttatta ttattgccgt
    15721 gttaggctat atctattcaa gtcagactac agagtgggcg attttaatct atggtctggt
    15781 gatgatcttc tttttataca tgtatgtttg cttcgcgtcg gcggtttata tcccggagct
    15841 ttggccaacg catttacgcc tgcgcggttc gggtttcgtt aatgccgtcg gacggatcgt
    15901 cgcagttttc acgccctatg gcgttgcggc attattaaca cattatgggt cgatcacggt
    15961 gtttatggtg cttggtgtca tgttagtgct ctgtgcgctg gttctctcca tttttggcat
    16021 cgaaacgcgg aaggtgtcgc tggaagagat ttctgaggtg aattaaatct ttcgttgatg
    16081 gcatcaattg ccagatgtac cactgacgtg ttttatttgg cctacaatgc cagtacagaa
    16141 ttgtaggcca gcgtaaaatt acccctgcgt ctcttcccag tctgccagcg tatatagcgt
    16201 tgccccagct gctgacatct ccataaacgc gtgtgcactg tcctggggct ggatattcac
    16261 gccacgacag ccatcggtaa tcacgtttac cttataacct aactgtaacg cgtccagcac
    16321 ggtaaacttc acgcaatagt cagtagccag gcccataacg atcaattcat cgatttcatg
    16381 atcgcgcaac cagtcatcaa gcgcggtttt ctgccgacgt ccgttatcaa aaaaggcact
    16441 gtaactgtca actaaaggat tttcgccttt atggaacacc gctgcgatct ctttttgatt
    16501 cagtaacgga tgtaattgtg cgccttcact gttctgcaca cagtgttcag gccagaaagt
    16561 ttgccgcagg ccgtcgagtt ggcctggcgt ataaggctct acgccg
//
LOCUS       DADLSB010000087        16281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.087, whole genome shotgun sequence.
ACCESSION   DADLSB010000087 DADLSB010000000
VERSION     DADLSB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.087"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            7..1737
                     /locus_tag="JN331_002614"
     CDS             7..1737
                     /locus_tag="JN331_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260732.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQLEKAMQLPSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVMPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYATNGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKLL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(1797..2288)
                     /gene="yajQ"
                     /locus_tag="JN331_002615"
     CDS             complement(1797..2288)
                     /gene="yajQ"
                     /locus_tag="JN331_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY7260733.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2456..3367
                     /gene="panE"
                     /locus_tag="JN331_002616"
     CDS             2456..3367
                     /gene="panE"
                     /locus_tag="JN331_002616"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY7260734.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3330..3920
                     /gene="yajL"
                     /locus_tag="JN331_002617"
     CDS             3330..3920
                     /gene="yajL"
                     /locus_tag="JN331_002617"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY7260735.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3974..5422)
                     /gene="thiI"
                     /locus_tag="JN331_002618"
     CDS             complement(3974..5422)
                     /gene="thiI"
                     /locus_tag="JN331_002618"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY7260736.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5628..5870
                     /gene="xseB"
                     /locus_tag="JN331_002619"
     CDS             5628..5870
                     /gene="xseB"
                     /locus_tag="JN331_002619"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY7260737.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5870..6769
                     /gene="ispA"
                     /locus_tag="JN331_002620"
     CDS             5870..6769
                     /gene="ispA"
                     /locus_tag="JN331_002620"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY7260738.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6794..8656
                     /gene="dxs"
                     /locus_tag="JN331_002621"
     CDS             6794..8656
                     /gene="dxs"
                     /locus_tag="JN331_002621"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY7260739.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSCKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8711..9685
                     /gene="yajO"
                     /locus_tag="JN331_002622"
     CDS             8711..9685
                     /gene="yajO"
                     /locus_tag="JN331_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY7260740.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9739..10257)
                     /gene="pgpA"
                     /locus_tag="JN331_002623"
     CDS             complement(9739..10257)
                     /gene="pgpA"
                     /locus_tag="JN331_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY7260741.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10235..11212)
                     /gene="thiL"
                     /locus_tag="JN331_002624"
     CDS             complement(10235..11212)
                     /gene="thiL"
                     /locus_tag="JN331_002624"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY7260742.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKSGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11290..11709)
                     /gene="nusB"
                     /locus_tag="JN331_002625"
     CDS             complement(11290..11709)
                     /gene="nusB"
                     /locus_tag="JN331_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY7260743.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11729..12199)
                     /gene="ribE"
                     /locus_tag="JN331_002626"
     CDS             complement(11729..12199)
                     /gene="ribE"
                     /locus_tag="JN331_002626"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY7260744.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLE
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12288..13391)
                     /gene="ribD"
                     /locus_tag="JN331_002627"
     CDS             complement(12288..13391)
                     /gene="ribD"
                     /locus_tag="JN331_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY7260745.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRYVGPDVCLHLVGA"
     gene            complement(13395..13844)
                     /gene="nrdR"
                     /locus_tag="JN331_002628"
     CDS             complement(13395..13844)
                     /gene="nrdR"
                     /locus_tag="JN331_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY7260746.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13995..14534
                     /locus_tag="JN331_002629"
     CDS             13995..14534
                     /locus_tag="JN331_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7260747.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14833..15717
                     /locus_tag="JN331_002630"
     CDS             14833..15717
                     /locus_tag="JN331_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY7260748.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15894..16241)
                     /locus_tag="JN331_002631"
     CDS             complement(15894..16241)
                     /locus_tag="JN331_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY7260749.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
BASE COUNT         4180 a         4163 c         4034 g         3904 t
ORIGIN      
        1 tttgcgatga aagcgaaatc tactttgtta ttaatgattt tatctgtttt ttattctcat
       61 caggttttat ccagcgaacc cactcagccg gatgtttatt ctgtcgtcga gaaaaaacta
      121 aactctgctt tacctctgga agaaaatacc accttcagat cgcaggctga atggtttaat
      181 ttgcaatatg aattattagt atctggctac cctgaacgtg cttacaattt actgagcaaa
      241 cttgagacag aatcgaaaaa cgctaaatat tatctggatt taactgcctc cagagtcgct
      301 gaagaagaag caccagagac agccttaact tttttatcaa aaattgggtt aaataaaccg
      361 gctactctta catcctttga gagctatatc aatagctgga taaaaagcaa acaactcgaa
      421 aaggctatgc agcttcccag cctcgatgag aatgcactga attattattt accgactgtg
      481 ttagtcgcat atcagatcgt tcctgataag gcaataagta tttataattc tgtatatgga
      541 gataatgttg ttatgcccta tcagcaagtc accatgttac ttacagttgc ggagggttat
      601 caaacaaaag gcgatataaa aaacgccatt cattatgccg ataaggcgct gaatatgttt
      661 gataaagcta ttgctgtaga tagcagtcgt gaatcccatt atagtgatga gtatttgaaa
      721 ctcatgaata tctacgccac caatggcaac aaagaaaaag cggtggtttt atctcaacgt
      781 ctacacaaag ccattaccga atctcaatcg aattacttaa gtactctgga aggaatttta
      841 ttgttttata gaaataatga tatgcagcaa gactatcaaa acagcctttc aaattacata
      901 gtatttatcg ataaaatttt tgccttttcc ccatcaacaa gaagtgaatt aagcctcatt
      961 aatttattaa attcattaaa tgaggtggaa ttgatgagaa aacttctggc gtttctgatg
     1021 acagcaccag aatacgcttg ttatgatagt cagtattgtt atgaagacaa agtcagcgca
     1081 ttaaaaatcc tgtatcgtca tcatgacaat gagctaatgg acaaatattt cacgactctt
     1141 cttaaagaga tcagtaatca accattagaa gactgggata taataataag taatactggt
     1201 gaaaaattta gtgaagtggg tctaaccaaa tatgctcaac atctcgcatc tttcgctgaa
     1261 gaattttata taaaagagca aaaatcacat tctgaaagtg aaatgagagg actattttca
     1321 tcacttgccg aattatatag ttttggtaat gatactgcca gcgcaaaccg agtgttccat
     1381 cagcatgtcc ctgccttcaa tacagatcat atgattcaat attttataaa tgccaaagaa
     1441 tggaacaatg ccagggaatt attgataaaa gaggaaatgt tgggtatgca taatctgata
     1501 ttgttggaga atatttgtat gcaaaaaaat gcagaatgca tggcacacat tacctttacg
     1561 cttaataaat taacaacaca accagcaata acaaaaatag atagcgtggg taatgagcaa
     1621 ctttatcaga ttggcaaaat ctaccataag ttggaaatta aacctgagcc agagcaacag
     1681 gttttaattc agtcgcttta tgaccgggca tcaggaagcg cctctgcaac tcaataaatg
     1741 catggtcaaa agggccacca cggtggccct gacaatcagt agctatatcc agaaagttaa
     1801 tcgcggaagt ttttgaactg gaacggctga ccgagatcgc caccgcgcac catcgccatg
     1861 acagcctgca aatcatcacg agatttgcca gtcacgcgga tctcatcgcc ctgaatttgc
     1921 gcctgcactt tcagtttgct gtctttgatc atcttgacga tttttttctg agtggcgctc
     1981 tcaatgccct gtttcagttt cgcttccaca aaccaggttt taccgctatg aacgatattt
     2041 tccggtacat ccagtgaact gccttcaatg ccgcgcttca gcaacttggc acgcagaata
     2101 tccagcaact gattgacctg gaagtcggac tcgctcaaca ctttgatggt tttgctggcg
     2161 tcgttcagct caaatgaggc ttcaacgtta cggaagtcaa aacgggactc cacttcgcga
     2221 ctcgcgttat cgaccgcgtt acgtgcttcc tgaagatcaa cttcagagac aatatcgaaa
     2281 gatggcatct tttcctctcc cttcattttt gatgcgaagc ataatacctg caaagttaaa
     2341 taaccaacag tcctgcacca ggacgtatcc cttaattgtg catagtcgca acagcatgca
     2401 caattaaggg atagccttat tgaacacccg gagtggttgc gggtgaggag gaacaatgaa
     2461 aattaccgta ttgggatgcg gtgccttagg gcaattatgg cttacagcac tttgtaaaca
     2521 gggtcatgaa gttcagggct ggctgcgcgt accgcaacct tattgtagtg tgaatctggt
     2581 tgagacagat ggttcgatat ttaacgaatc gctgaccgcc aacgatcccg attttctcgc
     2641 caccagcgat ctgctcctgg tgacgctgaa agcatggcag gtttccgatg ccgtcaaaag
     2701 cctcgcatcc acactgcctg taactacgcc aatactgtta attcacaacg gcatgggcac
     2761 catcgaagaa ttgcaaaaca ttcagcagcc attactgatg ggcaccacca cccatgccgc
     2821 ccgccgcgac ggcaatgtca ttattcatgt ggcaaacgga atcacgcata tcggtccggc
     2881 acggcaacag gatggcgatt acagttatct ggcggatatt ttgcaaaccg tgttgcctga
     2941 cgttgcctgg cataacaata ttcgcgccga gctgtggcgc aagctggccg tcaactgcgt
     3001 gattaatcca ctgactgcca tctggaattg cccgaacggt gaattacgtc atcatccgca
     3061 agaaattatg caaatatgcg aagaagtcgc ggcagtgatc gaacgcgaag ggcatcatac
     3121 ttcagcggaa gatttgcgtg attacgtgat gcaggtgatt gatgccacag cggaaaatat
     3181 ctcgtcgatg ttgcaggata tccgcgcgct gcgccacacc gaaatcgact atatcaatgg
     3241 ttttctctta cgccgcgccc gcgcgcatgg gattgccgta ccggaaaaca cccgcctgtt
     3301 tgaaatggta aaaagaaagg agagtgaata tgagcgcatc ggcactggtt tgcctcgccc
     3361 ctggtagtga agagactgaa gccgtcacca cgatcgacct cttggttcgc ggcggtatca
     3421 aagtcaccac tgccagcgtc gacagcgatg gcaacctggc aattacctgc tcgcgcggcg
     3481 tgaagctgct ggcggatgcg ccgctggtcg aagtggctga tggcgaatat gacgtgatcg
     3541 tgctgcctgg tggcattaaa ggcacggagt gttttcgcga tagcactctg ctggttgaaa
     3601 ccgttaaaca gttccaccgt tccgggcgta tcgtcgcggc tatttgcgcc gcgccagcca
     3661 ccgtgctggt gccgcacgat atctttccga ttggcaatat gaccggcttc ccgacgctga
     3721 aagacaaaat tcccgctgaa caatggcagg acaagcgcgt cgtctgggat gcacgggtaa
     3781 aattgctgac cagccaaggg ccgggtacag ctatcgactt tggtctgaaa attatcgacc
     3841 tgctggttgg gcgcgagaaa gcccatgaag tggcatcaca actggtgatg gcggcaggga
     3901 tttataatta ttacgagtag tgtcggatgc ggcaaacgtc gcatctgacc agatgcgacg
     3961 taaaaactac aaattacggg cgatatacct tcacattgtt aaagccttgc tcgcgcagat
     4021 agagcgcctg cagacggctc atcaccccgc gctcacacca cagcagccag gttttgttct
     4081 ggtcgagatc gccaaatttg gtgctcagtt tatagaacgg cagagaaacc acatcaatcc
     4141 cttcgacttt cagtggctta tcttcctgtt catcgacaga acggatatcg agaatcacgt
     4201 cgttcgggcc gaagccgttg acggtttcca cttccaccac ttcctgctcg gtctgctggg
     4261 cgatttcgcg gatatcaacg ttattcgctt cctcaaccac tttatcgaga atactgaagt
     4321 cgaacttctc ttcttccgct tcaatcttcg atttaaccgc tttcaccgtc gggcttttgg
     4381 agatcacgcc acaatattcc ggcatcgtgc gagcaaagtc ttcggtgcca atctggcgag
     4441 ccaggttgat gatgtgctct ttgtcgtaag aaatcagcgg acgcaggatc agcgtgtcag
     4501 agacgttatc aatcaggcgc aggttggtca gcgtctgact ggacacctgg ccgagcgctt
     4561 cgccggtgac cagcgcctgt acgccgtaac gttcagccac tttagatgcg gcacgcacca
     4621 tcatacgttt gaggataacg cccatctgac cgtcgtcgat tttctcgaga atttccccga
     4681 cgaccggttc gaaattaatg gcgacaaaac gcacgcggtg ggagctgcca aagcggttcc
     4741 acagataatg cgccacctga cgcacgccaa tttcatgcgc tgcgccgccg aggttaaaga
     4801 agcagtaatg cacgcggcag ccgcgacgca tcaacatata actggaaaca ccggagtcga
     4861 aaccaccgga aatgagcgac agcacatctt cctgggtgcc aatcgggaaa ccaccaatac
     4921 cttcgtagcg acctttaatc agcaggaggc gatcgtcttc cacttccaga tgaacagtta
     4981 catccgggtt agtcagcttc acgcgcgcgg attcaatatg ctgatttaaa ccgccgccga
     5041 cgtaacgttc cacatcaatc gagctaaaat catgtttgcc acggcgcttc acgcgtacgc
     5101 agaaggtttt gccttccagc tgatcgcgat actgaaccaa cgctttctca aaaatatcgt
     5161 gcatgtcggt aaacggcacg tcttcgactt cgagaatatg gtggataccc ggaatacggg
     5221 tcagggcgtc gcgaatagtc agacgctggt tttcatcttt cgcgcgaact tcgatgttat
     5281 cccagtggcg gacgacagcg agcgtctcat catagtgctt taaaacgtta cgaatgttcc
     5341 cggtaaggat ttttataaag cgcaagcgca cagattggct tttgatggtg atttccggga
     5401 acaatttaat gataaacttc atggcggcaa tggttcgttg gcaagcctta agcgacttgt
     5461 atagggaaaa atacagcagc ccacacctgc ggctgcatcc aggcgcggaa gtataccact
     5521 aacatcgctt tgctgcgcac atcaccttac cattgcgcgt tatttgctat ttgccctgag
     5581 tccgttacca tgacggggcg aaaaatattg agagtcagac attcattatg ccgaagaaaa
     5641 atgaggcgcc cgccagcttt gaaaaggcgc tgagcgagct ggaacagatt gtaacccgtc
     5701 tggaaagtgg cgacctgccg ctggaagagg cgctgaacga gttcgaacgc ggcgtgcagc
     5761 tggcacgtca ggggcaggcc aaattacaac aagccgaaca gcgcgtacaa attctgctgt
     5821 ctgacaatga agacgcctct ctaacccctt ttacaccgga caatgagtaa tggactttcc
     5881 gcagcaactc gaagcctgcg ttaagcaggc caaccaggcg ctgagccgtt ttatcgcccc
     5941 actgcccttt cagaacactc ccgtggtcga aaccatgcag tatggcgcat tattaggtgg
     6001 taagcgcctg cgacctttcc tggtttatgc caccggtcat atgtttggcg ttagcacaaa
     6061 cacgctggac gcacccgctg ctgccgtaga gtgtatccac gcttactcat taattcatga
     6121 tgatttaccg gcgatggatg atgacgatct gcgtcgcggt ttgccgacct gccatgtgaa
     6181 gtttggcgaa gcaaacgcga ttctcgctgg cgacgcttta caaacgctgg cgttctcgat
     6241 tctaagcgat gccaatatgc cggaagtgtc ggatcgcgac agaatttcga tgatttctga
     6301 actggcgagc gccagcggta ttgccggaat gtgcggtggt caggcattag atttagacgc
     6361 ggaaggcaaa cacgtacctc tggacgcgct tgagcgtatt catcgtcata aaaccggcgc
     6421 attgattcgc gccgccgttc gccttggtgc attaagcgcc ggagataaag ggcgtcgtgc
     6481 tctgccagta ctcgacaagt atgcagagag catcggcctt gccttccagg ttcaggatga
     6541 catcctggat gtggtaggag atactgcaac gttgggaaaa cgccagggtg ccgaccagca
     6601 acttggtaaa agtacctacc ctgcacttct gggtcttgag caagcccgga agaaagcccg
     6661 ggatctgatc gacgatgccc gtcagtcgct gaaacaactg gctgaacagt cactcgatac
     6721 ctcggcactg gaagcgctag cggactacat catccagcgt aataaataaa caataagtat
     6781 taataggccc ctgatgagtt ttgatattgc caaatacccg accctggcac tggtcgactc
     6841 cacccaggag ttacgactgt tgccgaaaga gagtttaccg aaactctgcg acgaactgcg
     6901 ccgctattta ctcgacagcg tgagccgttc cagcgggcac ttcgcctccg ggctgggcac
     6961 ggtcgaactg accgtggcgc tgcactatgt ctacaacacc ccatttgacc aattgatttg
     7021 ggatgtggga catcaggctt atccgcataa aattttgacc ggacgccgcg acaaaatcgg
     7081 caccatccgt cagaaaggcg gtctgcaccc gttcccgtgg cgcggcgaaa gcgaatatga
     7141 cgtattaagc gtcgggcatt catcaacctc catcagtgcc ggaattggta ttgcggttgc
     7201 tgccgagaaa gaaggcaaaa atcgccgcac cgtctgtgtg attggcgacg gcgcgattac
     7261 cgctggcatg gcgtttgaag cgatgaatca cgcgggcgat attcgtcctg atatgctggt
     7321 ggtcctcaac gacaatgaaa tgtcgatttc cgaaaatgtt ggcgcgctta acaaccatct
     7381 ggctcagctg ctttccggta agctttactc ttcactgcgc gaaggcggga aaaaagtttt
     7441 ctctggcgtg ccgcccatta aagagctgct caaacgtacc gaagaacata ttaaaggcat
     7501 ggtggtgcct ggcacgttgt ttgaagagct gggatttaac tacatcggcc cggtggacgg
     7561 tcacgatgtg ctggggctta tcaccacgct aaagaacatg cgcgacctga aaggcccgca
     7621 gttcctgcat atcatgacca aaaaaggtcg tggttatgaa ccggcagaaa aagacccgat
     7681 cactttccac gccgtgccta aatttgatcc ctccagcggt tgtttgccga aaagtagcgg
     7741 cggtttgccg agctattcaa aaatctttgg cgactggttg tgcgaaacgg cagcgaaaga
     7801 caacaagctg atggcgatta ctccggcgat gcgtgaaggt tccggcatgg tcgagttttc
     7861 atgtaaattc ccggatcgtt acttcgacgt ggctattgcc gagcaacacg cagtgacctt
     7921 tgccgccggt ctggcgattg gtggatacaa acccattgtc gcgatctact ccaccttcct
     7981 gcaacgcgcc tatgatcagg tgctgcatga cgtggcaatt caaaagctac cggtcctgtt
     8041 cgccattgac cgcgcgggca ttgttggtgc tgacggtcaa actcaccagg gcgcttttga
     8101 tctctcttac ctgcgctgca taccagaaat ggtcattatg accccgagcg atgaaaacga
     8161 atgtcgccag atgctctata ccggctatca ctataacgat ggcccgtccg cggtgcgcta
     8221 cccgcgcggc aacgcggttg gcgtggaact gacgccgctg gaaaaactac caattggcaa
     8281 aggcattgtg aagcgtcgtg gcgagaaact ggtgatcctt aactttggta cgctgatgcc
     8341 agaagcggcc aaagtcgccg aatcgctgaa cgctacgctg gtcgatatgc gttttgtgaa
     8401 accacttgat gaagcgttaa ttctggaaat ggccgccagc catgaagcgc tggtcaccgt
     8461 agaagaaaac gccattatgg gcggcgcagg tagcggagtg aacgaagtgc tgatggccca
     8521 tcgtaaacca gtacccgtgc tgaacattgg cctgccggac ttctttattc cgcaaggaac
     8581 tcaggaagaa atgcgcgccg aactcggcct cgatgccgcc ggtatggaag ccaaaatcaa
     8641 ggcctggctg gcataatccc tactccactc ctgctatgct taagaaatta ttcatggcag
     8701 gagtggcagc atgcaataca accccttagg aaaaaccgac cttcgcgttt cccgactttg
     8761 cctcggctgt atgacctttg gcgagccaga tcgcggtaat cacgcatgga cactgccgga
     8821 agagagcagc cgtcccataa ttaaacgcgc gctggaaggt ggcataaatt tctttgatac
     8881 cgccaatagc tattccgatg gtagcagcga agagatcgtt ggtcgcgcac tgcgggattt
     8941 cgcccgtcgt gaagacgtag tcgttgccac caaagtgttc catcgcgttg gtgatttacc
     9001 ggaaggatta tcccgtgcac aaattttgcg ctctatcaac gacagcctgc gacgtctcgg
     9061 catggattat gtcgatatcc tgcaaattca tcgctgggat tacaacacgc cgatcgaaga
     9121 gacgctggaa gccctgaacg acgtggtaaa agccgggaaa gcgcgttata tcggcgcgtc
     9181 atccatgcac gcttcgcagt ttgctcaggc gctggaacta caaaaacagc actgctgggc
     9241 gcagtttgtc agtatgcagg atcactataa tctgatttat cgcgaagaag agcgcgagat
     9301 gctgcctctg tgttatcagg agggcgtggc ggtgattccg tggagcccgc tggcgcgggg
     9361 gcgactgacg cgtccatggg gagaaactac cgcacgactg gtgtctgatg aggtggggaa
     9421 aaatctctat aaagaaagcg atgaaaatga cgcgcagatc gcagagcggt taacgggcgt
     9481 cagtgaagaa ctcggtgcaa cacgagcaca agttgcgctg gcctggttgt taagtaaacc
     9541 gggcattgcc gcaccgatta tcggaacttc gcgggaagaa cagcttgatg agctgctgaa
     9601 cgcggtggat atcactttga agccagaaca gattgccgag ctggaaacgc cgtataaacc
     9661 gcatgcggtc gtaggattta aataagagtc caggcctgat gagacgtgac aagcgtcaca
     9721 tcaggcatcg gtgcacaact acgacagaat acccagcggc cagtgatgac cgataaagta
     9781 caggatgcct gcggaaatca ccccggcgac aatatcgtcg atcatgatcc ccatgccgcc
     9841 atgtacattg cgatcaaacc agcggatcgg ccacggcttc cacatatcca gaatacggaa
     9901 aatcacaaac ccggcggcaa cccactgcca gtcattggtc ggcagcgcca tgagcgtgat
     9961 ccacatacca ataaattcgt cccagacaat gctgccgtga tcgtgcacgc ccatatcttt
    10021 cgccgtttga tgacaaagat agacgccgat acagatcccc aacatcacca ccagcgagta
    10081 gagctgccag ggtaaaaagg tcatcagata ccagaaaggg atcgccgcca gcgatcccat
    10141 cgtgccagga acgattgggc ttaatccact tccgaatccg acagcaagca gatgccacgg
    10201 attactcatc ttcaggcgac ttttcgcgac atctttatgg cgtggcaaaa tggtcatatc
    10261 ctttccagtc aaacgtgaca ggttcgccgt cacgaataaa acaaagccct tcgatatcgg
    10321 cggtcatttg cccgatacag gtaaacggta cgccaaggtg tccgagagca acatccagcg
    10381 cgccacggtt cagctccggc accgtgaaac acaactcgta atcttcaccg ccagagagcg
    10441 cccagcgcag cgcctgttcc ggttcaacat ggcgagaaag cgcatcagaa aacggcagca
    10501 atgccaggtc aatacgtgcg ccgcagtcgc tggctttcac gatatgcccg agatcggaaa
    10561 tcagaccgtc agagagatcg atagctgaat ttgccagatc gcgcagtgcc tgcccctgta
    10621 aaatacgcgg cgatggacgg agatgacgtt tgatcaagta gtcagcatct ttagcatcgg
    10681 caacctgcaa acggttttgc aaaatcgcca gcccggcggc gctatcgccc ggtgtaccgg
    10741 tcacatagat ccagtcaccc gatttcgccc cagagcgcgt taaggctcgt cccatcggaa
    10801 caaagccgtg gatacccaac gtcattgata atggcccacg cgtggtatcg ccgccaatga
    10861 gttgcatatc gtaataattg agaagatcaa acaaactgtc gctgaaggac tcaagccacg
    10921 cttcgtctac gtccggtaag gttaatgcca gcgtcagcca ggccggatcg gcccccatcg
    10981 ctgccagatc gcttaggttc accgccagtg ctttataagc cagatcagca ggatcgatat
    11041 cagggaggaa atggttgccc gccaccagcg tatcagtgct gatcgccagg gtctgtttct
    11101 cggggatatt gagaagagcg caatcgtcgc cgatgcccag ttcgacatca agacgagaac
    11161 ttcttacacg gtcaaaataa cgggcaatca gggagaactc gccacatgcc atacgttatg
    11221 cctcagcagg taaaaaagaa aaggccggcg atcatggaac agtcttccgt gaatctaccg
    11281 gcctggatat tactttttgt tagggcgaat cacaggtgct gctttatcga gtacgccgtt
    11341 gacgaactta tggctgtctt ctgcgccgaa cgatttcgcc agttcgatcg cttcgttaat
    11401 ggccactttg tatggcacat cactacgttt agacagttcg tacagcgcaa tgcgcagtac
    11461 tgctttttct acctggccca gttcttccag caggcgggac aggtagggct tcatcagtcc
    11521 gtcgaggtat gcggtgttag tcgccacccc ggccagcagc tcacggaagt acaggacgtc
    11581 aacgtctttt acatcctgtt cagccaggaa ctggtattca acatcagcga tgtcgttctg
    11641 ggacaactgc caggagtaga gcgcctggac ggcacactca cgagcgcggc gacgagcagc
    11701 aggtttcacg gatttcccct tactaatttc aggccttgat ggctttcaat acattaatca
    11761 tttcaagcgc ggtcagtgca gcttctgcac ctttgttgcc agctttggtg ccagcacgtt
    11821 cgatcgcttg ttcaatgctt tcagtggtca gaaccccaaa agcaaccgga atttcgctgt
    11881 cctgggcaac atgctccagg ccgttgcttg caccaccagc gacatattca aagtgggcag
    11941 tgccaccacg aataaccgta cccagcgcaa tcaccgcgtc gtatttaccg gttttagcca
    12001 gtgcacccgc cgccagcggc agctcatagg caccaggcac ccaaacaacg gtaatgtttt
    12061 catcttttac ctgaccgata cgtttcagtg cgtcaattgc accttccagc aggctgtcat
    12121 tgataaagtt gttgaaacgc gcgatggtga tggcgacgcg agcgtccggg gtagcaacgt
    12181 tagcttcaat aatgttcatt ttcttccttc gggttcgagt atggccccgc aggggggcgg
    12241 attttagcat aatatttcgt gcgctgcttc cctttcgagc cgggagatca tgcacccact
    12301 aaatgcaggc aaacatccgg gcctacataa cgtatctctt tgaatttaaa ttggggggcg
    12361 tcggctaatt tctcaagccc tggcagcgag cataatccac gggcgtcgct gcctaatagt
    12421 ttaggtgcga tatagacaat cagctcatcg actaaacccg cctgtagcaa tacgccagcg
    12481 agcgttggcc ccgcttccac ccagatgctg ttaatctgct gtttacccag ttgcatcatc
    12541 agtacaacca gatccagatg acctttatgc tctggaatca gcaaggtacg caccgtttcc
    12601 ggccactcac gagaatcttc ctgcgtacgc gcgaaccagg tttcgccggg ctgctgcaca
    12661 atgcgatgtt ccggcgtcac gcgattttgg ctatcaatca caatacgtac tggctggcgg
    12721 agattttgtt gcgggtagag cgcctgagtt tgttcatcca gttcagacca acgcactgtt
    12781 aaggctggat catccgccag caccgtggcg ctgctggtta aaatggcatg actttgtgcg
    12841 cgctgtcgtt gtacgtcgcg ccgcgcctga ggcgaagtga tccactggct ttcgccgctc
    12901 gccatcgccg tgcggccatc aagcgatgcg ccaagtttta actgaatata aggaaagccg
    12961 gtgcgcatcc gcttgagaaa gcctttattc aattgctcgg cttcactcat catcagcccg
    13021 tggctgacgt caatgccagc ctgttgcaga cggtaaagtc cacgcccagc gacctgcggg
    13081 ttaggatctt gcatcgctgc gaccacgcgc gctacgccag cggcgatgag tgcgtcacag
    13141 cacggtggcg tacggccatg atggctacag ggttcgagtg tcacatacgc cgttgcgcct
    13201 ttggcttttt cacccgccat acgcaacgcg tgtacttcgg catgtggttc acccgctcgt
    13261 tggtggtaac cttcaccgac aatttcgcca tctttgacaa tgacgcaccc gacattcggg
    13321 ttggggtggg tggtaaaacg tcctcgttgc gccagcttta gcgcccgcgc catgtaatac
    13381 tcgtcctgca cggcttagtc ctccaggcgc gcgatctctt cgccaaattc tttgatatct
    13441 tcgaaactgc ggtagacaga ggcaaaacgg atataggcga ctttatcgag ctttttcaat
    13501 tgctccatca ccagattgcc aatcatcttg ctcggtactt cgcgctcacc ggtggcgcgc
    13561 agctgcgatt taatatgatt gattgccatt tcaacgtaat cggaactcac cggacgtttt
    13621 tccagcgccc gcagcattcc gctacgcaat ttctcttcat taaacggttc acgcacgtcg
    13681 ttgcttttta caacacgcgg cataaccagc tccgccactt caaaggtggt gaaacgttca
    13741 ttacacacca gacactgccg acggcggcgt acggatgaac cctcgcccac gagacgagag
    13801 tcaattacct tagtgtccac ggcgaaacag aatgggcaat gcatacggcg tcctgaccag
    13861 gtggttaacg gaaatctatt ttaccctgaa ctgacgatac aacaaaggcg caacgctttt
    13921 gagataaagg atcgatgctt tcgctgcttt taccctctac cattaggcta attgcgacga
    13981 gaaagggacg acctatgaat actaacgttt ttcgactgct cctgctggga agcctgttca
    14041 gccttagcgc ctgtgtgcag caaagtgaag tgcgacagat gaaacacagc gtcagcacgc
    14101 tgaaccagga gatgacgcaa ctcaatcaag aaacagtcaa aatcactcag caaaacaggc
    14161 tgaatgcaaa atccagcagt ggggtttacc ttctgcctgg agcgaaaaca ccggcaagac
    14221 tggaaagcca gatcggtact ttacgtatgt cgctggtgaa tattacgcct gatacagatg
    14281 gcaccaccct tacactacgt attcagggtg agtcgaatga tcccttgccc gcattcagcg
    14341 gaactgttga atatgggcaa attcagggaa caatagacaa ctttcaggaa atcaatgtgc
    14401 agaatcaatt gattaatgcc cctgccagcg tcctcgcccc cagcgatgtt gatattccgt
    14461 tacaattaaa ggggatttct gtggatcagt taggctttgt acgtatccac gacattcaac
    14521 ctgttatgca gtaaacgtat ttcgggacga ttttgtgcgt cccgcaacat cttttcccgt
    14581 cattttgtta ctctgcttac atcacctgga ttgatagtaa aagtttgcaa caagggcgaa
    14641 agtcagtaca atccccgccc gaatgtgtgt aaacgtgaac gcaatcgatt acgtaaatga
    14701 tagaactgtg aaacgaaaca tatttttgtg agcaatgatt tttataatag gctcctctgt
    14761 atacgaaata tttagaaacg caatttgcgc ctttttcact cccgcaaggg attttcaaac
    14821 agtggcatac atatgaaaaa aacattactg gcagccggtg cggtactggc gctctcttcg
    14881 tcttttactg tcaacgcagc tgaaaacgac aaaccgcagt atctttccga ctggtggcac
    14941 cagagcgtta acgttgtcgg aagctatcac acccgtttcg gaccgcagat ccgcaacgat
    15001 acctaccttg agtacgaagc attcgctaaa aaagactggt tcgacttcta tggttatgcg
    15061 gatgcgccgg tattcttcgg cggtaactcc gatgctaaag gtatctggaa ccacggttct
    15121 ccgctgttta tggaaatcga accacgtttc tccatcgaca agctgaccaa tactgacctt
    15181 agcttcggtc cgttcaaaga gtggtacttc gcgaacaact acatttacga catgggtcgt
    15241 aataaagatg gtcgccagag cacctggtac atgggtctgg gtaccgatat cgacactggc
    15301 ctgccgatga gcctgtccat gaacgtctat gcgaaatacc agtggcagaa ctatggcgca
    15361 gcgaacgaaa acgagtggga cggttaccgt ttcaaaatta aatactttgt gccgattacc
    15421 gatctgtggg gcggtcagct gagctacatc ggcttcacca acttcgactg gggttccgat
    15481 ttaggggatg acagcggtaa cgcaatcaac ggtattaaga cccgtactaa taactctatc
    15541 gcttccagcc atattctggc tctgaactac gatcactggc actactctgt cgtagctcgt
    15601 tactggcacg acggtggtca gtggaacgac gatgcagaac tgaacttcgg caacggcaac
    15661 ttcaacgttc gctctaccgg ctggggtggt tacctggtag taggttacaa cttctgatta
    15721 tgaaaatgcc gggatttatt cccggcattt ctgattgtta acaacgtgtg ctttgttcat
    15781 gccggatgcg gcgtaaacgc tttgtccggc ccacaaaagc acgtaaattc aatatattgc
    15841 tgaaataacg taggcctgat aagcgtagcg catcaggcaa ttttacgttt taatcacttc
    15901 ttcttgttca tcatcgcttt caggtcagcg aatgggttgt acttcgcttc accgacatct
    15961 ttctgcgcat cttcccctgc gataacggtc gtaccatact gatccgcttc ggtatatttc
    16021 gaatgctcat gatcgtggca atagagacac aacaattccc agttactgcc atcttccggg
    16081 ttattggtat ggtcgtgatc aatgtggtga acggtaagtt cacgcaggtt agaataaaca
    16141 aactcgcggg aacagcgacc gcagacccac ggatagattt ttaatgcttt ttcgcgatag
    16201 ccgctttcta accgtgcata gttttttggg atgatagcca tgaaaagtta cctggatgaa
    16261 atgtagggtt acgcgcagtt t
//
LOCUS       DADLSB010000088        16069 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.088, whole genome shotgun sequence.
ACCESSION   DADLSB010000088 DADLSB010000000
VERSION     DADLSB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.088"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1100
                     /locus_tag="JN331_002632"
     CDS             <1..1100
                     /locus_tag="JN331_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HAY7260750.1"
                     /translation="KVVGLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIK
                     GVGEGPIEAIIEARNKGGYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAA
                     LMNSLGDALKAADQHAKAEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERE
                     TLGLYLTGHPINQYLKEIERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNR
                     IGICTLDDRSGRLEVMLFTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVM
                     DIDEAREKYARGLAISLTDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLR
                     FGATWRVSPSDRLLNDLRGLIGSEQVELEFD"
     gene            1113..2072
                     /gene="accA"
                     /locus_tag="JN331_002633"
     CDS             1113..2072
                     /gene="accA"
                     /locus_tag="JN331_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY7260751.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            2171..4312
                     /gene="ldcC"
                     /locus_tag="JN331_002634"
     CDS             2171..4312
                     /gene="ldcC"
                     /locus_tag="JN331_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY7260752.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            4369..4758
                     /locus_tag="JN331_002635"
     CDS             4369..4758
                     /locus_tag="JN331_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7260753.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            4823..6121
                     /gene="tilS"
                     /locus_tag="JN331_002636"
     CDS             4823..6121
                     /gene="tilS"
                     /locus_tag="JN331_002636"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY7260754.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(6170..6430)
                     /gene="rof"
                     /locus_tag="JN331_002637"
     CDS             complement(6170..6430)
                     /gene="rof"
                     /locus_tag="JN331_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY7260755.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(6417..6617)
                     /locus_tag="JN331_002638"
     CDS             complement(6417..6617)
                     /locus_tag="JN331_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY7260756.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            6783..7328
                     /locus_tag="JN331_002639"
     CDS             6783..7328
                     /locus_tag="JN331_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY7260757.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            7325..7747
                     /gene="arfB"
                     /locus_tag="JN331_002640"
     CDS             7325..7747
                     /gene="arfB"
                     /locus_tag="JN331_002640"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7260758.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            7761..8471
                     /gene="nlpE"
                     /locus_tag="JN331_002641"
                     /gene_synonym="cutF"
     CDS             7761..8471
                     /gene="nlpE"
                     /locus_tag="JN331_002641"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY7260759.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(8626..9450)
                     /locus_tag="JN331_002642"
     CDS             complement(8626..9450)
                     /locus_tag="JN331_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY7260760.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(9503..11221)
                     /gene="proS"
                     /locus_tag="JN331_002643"
     CDS             complement(9503..11221)
                     /gene="proS"
                     /locus_tag="JN331_002643"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY7260761.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYNKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(11332..12039)
                     /gene="tsaA"
                     /locus_tag="JN331_002644"
     CDS             complement(11332..12039)
                     /gene="tsaA"
                     /locus_tag="JN331_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY7260762.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(12036..12440)
                     /gene="rcsF"
                     /locus_tag="JN331_002645"
     CDS             complement(12036..12440)
                     /gene="rcsF"
                     /locus_tag="JN331_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY7260763.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(12558..13373)
                     /gene="metQ"
                     /locus_tag="JN331_002646"
     CDS             complement(12558..13373)
                     /gene="metQ"
                     /locus_tag="JN331_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY7260764.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(13413..14066)
                     /gene="metI"
                     /locus_tag="JN331_002647"
     CDS             complement(13413..14066)
                     /gene="metI"
                     /locus_tag="JN331_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY7260765.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(14059..15090)
                     /gene="metN"
                     /locus_tag="JN331_002648"
     CDS             complement(14059..15090)
                     /gene="metN"
                     /locus_tag="JN331_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY7260766.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDDIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            15278..15853
                     /gene="gmhB"
                     /locus_tag="JN331_002649"
     CDS             15278..15853
                     /gene="gmhB"
                     /locus_tag="JN331_002649"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY7260767.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3905 a         4040 c         4312 g         3812 t
ORIGIN      
        1 agaaggtggt gggcctggtg gatgagtgct ggcggatggg gctgaaaatc ctgccaccag
       61 atataaactc cggtctttac catttccacg tcaacgacga cggcgaaatc gtgtatggta
      121 ttggcgccat caaaggggta ggtgaaggtc cgattgaggc catcatcgaa gcccgtaata
      181 aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg taccgacacc aaaaagttaa
      241 accggcgggt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt gggccacacc
      301 gcgcggcgct gatgaactcg ctgggtgatg cgttaaaagc ggcagatcaa cacgcgaaag
      361 cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg gaacaaattg
      421 aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat ggggaacgtg
      481 aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa gagattgagc
      541 gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt aaagtcatca
      601 cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg
      661 gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt actgacgccc
      721 tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc ggacaggtca
      781 gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg
      841 aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg caaattgatg
      901 accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg acaattccag
      961 tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc gcgacgtggc
     1021 gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt tcggagcagg
     1081 tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt gattttgaac
     1141 agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc cgtcaggatg
     1201 agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac
     1261 tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg gcacgccatc
     1321 cgcagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt gacgaactgg
     1381 ctggcgaccg tgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg
     1441 gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc
     1501 gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg
     1561 ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct tatcctggcg
     1621 tgggcgcaga agagcgtggt cagtctgaag ccattgcacg caacctgcgt gaaatgtctc
     1681 gccttggcgt accggtagtt tgtacggtta tcggtgaagg tggttctggc ggtgcgctgg
     1741 cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc gttatctcgc
     1801 cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa agcgccgctg gcggctgaag
     1861 cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc atcatcccgg
     1921 aaccgctggg cggcgctcac cgtaacccgg aagcgatggc ggcatcgttg aaagcgcaac
     1981 tgctggcgga tctggccgat ctcgatgtgt taagcactga agatttaaaa aatcgtcgtt
     2041 atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa aagggtcact
     2101 tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa ggattttcca
     2161 ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt ataaagatga
     2221 gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta tctggccaca
     2281 aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg gcgtgatttt
     2341 tgactgggat gagtacagtc tcgatttatg cagcgatatc aatcaactta atgaatatct
     2401 cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc aggatatgcg
     2461 gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg ccattcgtat
     2521 gcgtcagtac accaacgaat atcttgataa cattacgccg ccgttcacga aagccttgtt
     2581 tacctacgtc aaagagcgga agtacacctt ttgtacgccg gggcatatgg gcggcaccgc
     2641 atatcaaaaa agcccggttg gctgtctgtt ttatgatttt ttcggcggga atacccttaa
     2701 ggctgatgtc tctatttcgg tcaccgagct tggttcgttg cttgaccaca ccgggccaca
     2761 cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt atatcgttac
     2821 caacggaaca tcgacgtcga acaaaattgt gggtatgtac gccgcgccat ccggcagtac
     2881 gctgttgatc gaccgcaact gccataaatc actggcgcat ctgttgatga tgaacgatgt
     2941 agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attctggggg ggatcccgcg
     3001 ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccactacgc aagcacaatg
     3061 gccggttcat gcggtgatca ccaactccac ctatgatggt ttgctctaca acaccgactg
     3121 gatcaaacag acgctggatg tcccgtcgat ccacttcgat tccgcctggg tgccgtatac
     3181 tcattttcat ccaatctacc agggtaaaag tggtatgagc ggcgagcgtg ttgcgggaaa
     3241 agttttcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc aggcttcact
     3301 gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta tgatgcatac
     3361 caccacctcg cccagttatc ccattgttgc ttcggttgag acggcggcgg cgatgctgcg
     3421 tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc attttcgcaa
     3481 agaggtccag cggctgcggg aagagtctga cggttggttt ttcgatatct ggcaaccgcc
     3541 gcaggtggat gaagccgaat gctggcccgt tgcgcctggc gaacaatggc acggctttaa
     3601 cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt tgacaccggg
     3661 gatggacgaa cagggcaata tgagtgagga ggggatcccg gcggcgctgg tagcaaaatt
     3721 cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc tgtttctctt
     3781 tagtattggc atcgataaaa ccaaagcaat gggattattg cgtgggttga cggaatttaa
     3841 gcgctcttac gatctcaacc tgcggatcaa aaatatgctg cccgatctct atgctgaaga
     3901 tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc ataagctgat
     3961 tcgtaaacac gatctttccg gtttgatgtt gcgggcattc gatactttgc cggaaatgat
     4021 catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa ccattgcgct
     4081 ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac cgggcgtacc
     4141 gctgttgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg attttctact
     4201 gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc acggcgcgaa
     4261 acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat aacttgccag
     4321 agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat gctgggttta
     4381 aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa agctttctac
     4441 tgcgatattc ttggcttcac gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg
     4501 aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcttt tccgttcccg
     4561 ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat
     4621 gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga agccatccgt
     4681 gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg gctgccgttg
     4741 gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt
     4801 gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc tcacctcacg
     4861 ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc atcagttggt
     4921 gcagtggcgg acggaaaatc cgggcgtcac tctgcgcgct attcatgtgc atcacggttt
     4981 aagtgctaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac agtggcaggt
     5041 gccgctggtg gtcgaacgcg tacaacttgc gcaagagggg ctggggattg aggcccaggc
     5101 gcggcaggcg cgttatcagg catttgcccg taccctattg cccggcgaag tgctggtcac
     5161 cgcgcaacat ctcgacgatc aatgtgaaac ctttctgctg gcgctaaaac gcggtagcgg
     5221 ccctgccggg ctttcggcta tggcggaagt ttcggagttt gctggaacac ggcttatccg
     5281 cccgttgctt gcccgcacgc ggggggaact ggagcagtgg gcattggcgc atggtttacg
     5341 ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc tgcgcctgcg
     5401 cgtagtgccg ttattgcagc agcgttggcc gcattttgcg gaagcaacgg cccgcagcgc
     5461 cgcactttgt gctgaacaag agagtctgtt ggatgaactg ctggcggatg atttagcaca
     5521 ctgccaaacg tcgcagggga cgctgcagat tgcgccaatg ctggcgatga gtgatgcccg
     5581 tcgcgcggcg attattcgcc gctggctggc agggcagaat gcaccgatgc cttcccgcga
     5641 cgcgctggtg cgcatctggc aggaagtggc gctggcgcgg gaagatgctt ccccctgttt
     5701 acgtttgggc gcctttgaaa tccgccgcta tcagtcgcaa ctgtggtgga ttaaatccgt
     5761 caccgggcaa agcgaaacca ttgtgctgtg gcagacgtgg ctacaaccgc tggaattacc
     5821 ggcggggctg ggaaccgtac agcttactgc gggaggcgat attcgccctc cgcgtgcaga
     5881 cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg catattgtcg ggcgcaacgg
     5941 cggacgtaag ctgaagaaaa tctggcaaga gttaggcgtg ccgccgtggc tacgtgacac
     6001 cacgccactg ctgttttatg gcgaaacgct gattgcggcg gcaggggtat ttgtgacgca
     6061 agaaggtgtg gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa cgcttagtta
     6121 agtgaaagcc ggataagacg catcaaacgt cgcatccggc gaaagtcaat caggattcgc
     6181 ttaccaccac cgtaccgatt tcagggtggc taaagctggt aattttatcc agacgcagct
     6241 cgcgggtttc gccagcggcc tcgacgacca gatactccac atttttgcgg gagactaaat
     6301 cactggcttt tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc attaaatgat
     6361 gctggcaggc gagctcaaga ttatcgtaat catcacaatt aattggttga tacgtatcat
     6421 tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt ttccctgaca
     6481 gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac gcagcccagc
     6541 gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt gcgtattaac
     6601 tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg gatgcaagtc
     6661 taagcctacg aagataaact ctgtttcgca aggtgactat accacactca tttctgcaat
     6721 atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa aggagttttc
     6781 ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat ctcgaccgca
     6841 accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta
     6901 tgatgctgcg cttgctggcg tggctgaaat atgctgatga acgtctgcaa tttacccgtg
     6961 gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt
     7021 tgtggattga gctggggctg ccggatgagc gacgaattaa gaaagcctgc acccaggccg
     7081 cagaagtggc gctgtttacc tataatagtc gggcggcgca aatctggtgg cagcaaaatc
     7141 agagcaaatg tgtgcagttt gccaatcttt ccgtctggta tctggacgat gaacaactgg
     7201 cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt caggatggtg
     7261 ttatctggtt atcggatgat aagaataatc tggaagttaa cttaaccgcc tggcaacaac
     7321 cttcatgatt gtgatttccc gacatgttgc tattcccgat ggtgagcttg agatcaccgc
     7381 cattcgtgcg cagggcgctg gcgggcagca tgttaataag acctcaacgg ctattcatct
     7441 gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagagcgtc tgctcgccgc
     7501 cagccatcat ttgatcagca gtgatggcgt gattgtcatt aaggcacagg aataccgcag
     7561 tcaggaactg aaccgcgaag ccgctctggc ccggctggtg gcagtgatta aagatttaac
     7621 aaaagaacaa aaagcccgac gacccacgcg gcccacccgt gcatcgaaag agcgcaggct
     7681 ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtac gcagcggtcg
     7741 ggaataaaaa gaaggaatgg atggtgaaaa aagcgatagt gacagcgatg gctgtaatca
     7801 gcctctttac tctgatggga tgtaataatc gggccgaagt cgatacgctt tctccggcgc
     7861 aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctaccg tgtgccgatt
     7921 gcgaaggaat cgaaatctct ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc
     7981 gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa
     8041 ccgctgacaa gctggtatta accgacagca aaggtgaaaa gtcatattat cgggcgaaag
     8101 gcgatgcgct ggagatgctc gatcgtgaag gcaatccaat tgaatcgcag ttcaactata
     8161 cgctggaacc ggcacaatcc agtttaccta tgacgccgat gaccctgcga ggcatgtatt
     8221 tttatatggc tgatgcggcg accttcactg attgcgcgac cggaaaacgt ttcatggtag
     8281 cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt gaaaaaccgg
     8341 tgttactgtc agtagaaggt cactttacgc ttgaggctaa tccggatact ggtgcgccaa
     8401 ccaaagtatt agcgcccgat acggcgggta aattttaccc gaataaggat tgcagtagtt
     8461 tggggcagta aacctgctta aggcagaaac aaacgcaaaa ttgcctgatg cgctgcgctt
     8521 atcaggccta cagcatataa tgcaacattt tgaattagca cgattttgta ggccggataa
     8581 ggcgtttacg ccgcatccgg cattttgtgc acgaggctgg ctttatcact gagtcagccc
     8641 aacaaagcga cccgccttga tataaatccc aggcttcagg tgcccaacat actgtaactg
     8701 cgtttctggc ttaaacgcag agacatcacc aatacgcata tgcatcagat cggcagggct
     8761 aatccagccc gattgcgcca gtgtaagtgg atgcccggct ttggcaaatg catcagtgac
     8821 aaattccgaa caaaaccacg actttttgtc tccttcgcac acactgctta actgcgcttt
     8881 cgccaggccg ctgacgcact gttggcgaaa atcctcggag aacggattca gtgagcacat
     8941 ctggcgagtc accataaagg gaataaattc gacaatgccg cgatagttat aaccgctatc
     9001 tttgatttta ttggcaaacg cggtgatatc tgtggcttgt tgcggggtaa gatccgggac
     9061 tcgtaagacg aaaagcttat cactatgctt catcgctttt ttaagggaaa caatctggac
     9121 gccagcgcct gtcgcttctg caacgttatt atcacccaga aagattgcaa cgtgactcac
     9181 agaggaagtg ctgaagacgc ggattccaaa tgaggttacc ccaaggcttg aggagaacag
     9241 caaatcgccg ggtttgagat caggtgccgt tatttctttt attgattgtt cggtgaaaga
     9301 gctttgatgc tggaatttga cagcccatgt ttttgcctcg gcatctactg ctgtggctga
     9361 tgggtctggc tggctgatat caacggtaca agccgaaagt aaaagaaagc tggggagaag
     9421 caggcggcag tacgcctttg gtttatccat tttatacaat ccatgtaaaa aagggccctg
     9481 aaattcagga ccctttctgg catcagcctt taatctgttt caccagatat tcgacgatgt
     9541 caccagtctt aattaactgt ttctcgccgt tgcgacgata tttatattcg atatcgtcgt
     9601 tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat accgatcagt tccatatcag
     9661 caaacatcac gcccggacgc tctttgcggt catccagcag cacttcgata ccttgtgcac
     9721 gcagttcgct gtacagtttc tcagcaagct cttgtacgcg gaaggatttg tgcatgttca
     9781 tcggcagaat cgccacctgg aacggcgcga tagcgtcagg ccatacgatg ccgcgttcgt
     9841 cgtagttctg ctcaatcgcc gcagctacca cacgcgttac cccgataccg tagcaaccca
     9901 tcgtcaggat ttggttacgg ccatcttcac cctgtacgga ggctttcagt gcttcggagt
     9961 acttggtacc cagctggaag atgtgaccaa cttcgatacc acgtttgatc agcagcgtac
    10021 cctggccatc cgggcttgga tcgccagcca ccacgttacg gatatctgca acttccgggg
    10081 tagcgacatc gcgatcccag ttgataccga agtagtgttt accatcgatg ttagcaccag
    10141 cagcgaaatc actcatcgcc gcaacggtac ggtcaatcac caccggaatc ggcatgttta
    10201 ccggacccag tgaacccgga ccggctttaa ccacagcacg aatttcttct tcggtcgcga
    10261 aagtcagcgg gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc agctcgtgat
    10321 caccgcgcac cagcagcgca accagcggga agctactgcc ttcaaccgct ttaaccagca
    10381 gagtcttaac cgttttctca atcggcagat tgaactgttc aaccagttcc gcgatggttt
    10441 tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc ggttctttcg
    10501 gcgcgatagc ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg gagaagacca
    10561 catcgtcttc accgctctgc gccagcacct ggaattcgtg agaggcgctg ccgccgatag
    10621 aaccggtgtc ggcctgtacg gcgcggaaat ccagccccat gcggctgaag attttgttgt
    10681 aggccgcata cattgcatcg taggtttcct gcagggattc ctgagaagta tggaaagagt
    10741 aagcatcttt catcaggaat tcgcgggaac gcatgacgcc gaaacgcgga cgcacttcgt
    10801 cgcggaactt ggtctggatc tgatagaagt tcagcggcag ctgtttgtaa gagctaagct
    10861 cgttacgaat caggtcagtg ataacttctt catgagttgg gccgagtacg aacggacgct
    10921 cgccacggtc aacaaaacgc agcagttccg gaccgtactg ttcccaacga ccactctctt
    10981 gccacaaatc ggctggctga accaccggca tcgacacctc gatcgcaccg gcgttgttca
    11041 tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc ggcagccagg
    11101 tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc tgatggctga
    11161 tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg ctagtacgca
    11221 tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa
    11281 gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg
    11341 ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg
    11401 tagccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc
    11461 ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct ccagcgtcaa
    11521 aagctgcttt tcgacctctg cggtaaaact caccgccatc tctgcagctg gcgcactttg
    11581 cgcatagctg gcactggcat cgggaagcga ttcggcaaag gggagatacg gtttgatatc
    11641 cactaccggc gtaccatcga ccagatccag actgccgagc ttcagaatca cgctgtcttt
    11701 atggcaaaca acctctttca gctctaccag cgacatgcca attgggttag ggcggaaagt
    11761 agagcgtgtt gcgaaaaccc ccattctggc gttaccgccg aggcgcggtg gacgcacagt
    11821 cggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata aatggctgaa
    11881 tgcttccagg ccgcgaacgg cgtcggcctg gttgtaggaa gcaatgagat gcagttcgcc
    11941 gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttcct tatagggcga
    12001 gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtaat gttaagcgca
    12061 gagccgatac atacagcctg acgatagcag cccggcgtac cgctggtgac ttcgcagcta
    12121 tgcagtaata cagcattggc tttcatttta gaggcattga tttgcatctg cttacgtgcg
    12181 gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc actgacttca
    12241 ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat tcggaccggc
    12301 gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc agtgctttga
    12361 acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag tgctactaaa
    12421 cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc aatattgaat
    12481 caggagcttg taaaaatgac aagacgggca agcgcccgtc ctgaatgata ttacaaattg
    12541 tggaaacagc ctaaaaatta ccagccttta acagcgccgc cgttaaacac tttgtttgct
    12601 gcttcgtaaa cttcatcaga ctgataagcc tggacgaatt tcttcacgtt ctcggcgtct
    12661 ttgttatctt cacgcgttac gatcaggttt acgtatgggg agtctttatc ttcgacaaag
    12721 ataccgtctt tcgccggagt caggccaatc tggctggcat aggtggtatt gataactgcc
    12781 agagcgattt gcgcgtcgtc cagagaacgc ggcagttgcg gtgcttccag ttcaacaatt
    12841 ttcagatttt ttgggttctc aacaacatca agaacggtcg gcagcaggcc aacgccatct
    12901 ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag attagttgga
    12961 tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt gattttcttg
    13021 gagtaaccag caatcggata aacaaaagtg ttgcctactg cgaccagttt gtagccacga
    13081 tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc gatatcgcct
    13141 ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg ttaccagctc aacgtccagg
    13201 ccatatttgt ctttcgcaac tttctgcgcg acttctgcta cctgctgttc ggcaccaaca
    13261 atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc taccagtgcc
    13321 agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc cataccttat
    13381 tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg
    13441 cggtcgcctg cgaactgaat taaataaacc agaatgacca gcaataccag taccgtattc
    13501 atcaccgtcg cgttgtagcc gatgtagcca tactgatagc caatctgacc taaaccaccg
    13561 gcaccgactg caccacccat cgcggaataa ccgaccaggg taatcagggt gatagttgcc
    13621 gcattcacca gacccggcaa cgcttccggt aacagcactt tacggacgat ctgcattggc
    13681 gtggcaccca ttgcgcggga agcttcaatt aacccggttg ggatctccag cagagcgttc
    13741 tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat tgctgcctgc
    13801 aaaccaatcg atgtaccgac aataacgcgg gtaaacggaa tcatccatac cagcaagata
    13861 atgaacggga tggaacggaa aatgttcaca atcgcagaaa cggtacgata cagcttcgcg
    13921 ttagcaataa tttgccccgg acgcgtgaca taaagcagaa cgccaaccgg cagaccaatc
    13981 acaaacccaa aaaatccgga tacgaaggtc attgccagcg tttcccatac gccacgaacc
    14041 agcagccaca tcatcggctc agacataacc cagtacctct acttttacat ggtgttcctg
    14101 cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag tcagcatgat
    14161 gccgaattta acgccaccgg cgtagtccat ctgcgcgcta ataatgttgt tgttgacgtt
    14221 gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg taaactccag
    14281 acgcagcatc ggcacgcagt cagtaaatgg ctccgcttgc agacgttcct ggtaatcttc
    14341 cgggatatcc agatgcaggg tcgactgaat aaacttctgc gccagcggcg ttttcggatg
    14401 cgagaacact tcacttaccg tgtcctgctc gatcagttcg ccattgctga tgaccgccac
    14461 gcaatcacaa atacgcttca caacgtccat ttcgtgggtg atcaacagaa tcgtcaaacc
    14521 cagacggcgg ttaatgtctt tcagcagttc gagaatagaa cgtgtcgttg ccgggtccag
    14581 cgcgctggtg gcttcatcac acagcaatac tttgggattg ctggctaatg cacgggcaat
    14641 cgccacacgt tgtttctgcc caccggaaag attcgacggg tagctatcat gcttatcgcc
    14701 aagaccaacc aatgacagca attccgtcac gcgacgtttg atatcgtctt tcggtgtgtt
    14761 gtccagctcc agcggcagag ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa
    14821 atgctggaaa atcataccaa tctggcggcg agctttggtc aactcggatt ctgacagcgt
    14881 ggtcaattcc tggccatcga ccagcacgct accctcggtt gggcgctcca gcaggtttac
    14941 acaacgtata agcgtactct tacccgcgcc tgaggcaccg ataacgccat aaatttgtcc
    15001 agctggcaca tgcaggctga cgttgttcaa cgcctggatg gtgcgggtgc cctggtggaa
    15061 cactttggtg atattcgaaa gttttatcat tgattattta ttatcgtcat taagttagtc
    15121 gtagcatctc gaatgcctga aacgggcaac gccgtcaatg aaatggatgt taaggcatcc
    15181 agacgtctaa atcaatcagg tttatgcgaa gaacactttc ttgcaggtcg aaacatgcga
    15241 tactagcgtc ccatgcctta ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat
    15301 ttttcttgac cgtgatggca ccattaatgt cgatcacggc tatgtccatg agatcgacaa
    15361 ctttgaattt atcgacggtg ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc
    15421 gctggtggta gtaaccaacc agtctggcat tgctcgcggt aaatttaccg aagcacagtt
    15481 tgaaacgctg accgagtgga tggactggtc gctggcggac cgagatgtcg atctggatgg
    15541 tatctattat tgcccgcatc atccgcaggg tagtgttgaa gagtttcgcc aggtctgcga
    15601 ttgccgcaaa ccacatccgg ggatgctttt gtcagcacgc gattatttgc atattgatat
    15661 ggccgcttct tatatggtgg gcgataaatt agaagatatg caggcagcgg ttgcggcgaa
    15721 cgtgggaaca aaagtgctgg tgcgtacggg taaacctatt acacctgaag cagaaaacgc
    15781 ggcagattgg gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa
    15841 accggcgcaa tgattaaaag atgaacggtt gaaataaaaa tgcatttttc cgcttgtctt
    15901 cctgagccga ctccctataa tgcgcctcca tcgacacggc ggatgtgaat cacttcacac
    15961 aaacagccgg ttcggttgaa gagaaaaaat cctgaaattc agggttgact ctgaaagagg
    16021 aaagtgtaat atacgccacc tcgcgacagt gagctgagag ctgcgtcgc
//
LOCUS       DADLSB010000089        15991 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.089, whole genome shotgun sequence.
ACCESSION   DADLSB010000089 DADLSB010000000
VERSION     DADLSB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.089"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..115)
                     /locus_tag="JN331_002650"
     CDS             complement(<1..115)
                     /locus_tag="JN331_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 domain-containing protein"
                     /protein_id="HAY7260768.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNGESALIIKMNDYGD"
     gene            complement(242..973)
                     /gene="rlmB"
                     /locus_tag="JN331_002651"
     CDS             complement(242..973)
                     /gene="rlmB"
                     /locus_tag="JN331_002651"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY7260769.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     TGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1153..3594)
                     /gene="rnr"
                     /locus_tag="JN331_002652"
     CDS             complement(1153..3594)
                     /gene="rnr"
                     /locus_tag="JN331_002652"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY7260770.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPNQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3633..4058)
                     /gene="nsrR"
                     /locus_tag="JN331_002653"
     CDS             complement(3633..4058)
                     /gene="nsrR"
                     /locus_tag="JN331_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY7260771.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4263..5561)
                     /gene="purA"
                     /locus_tag="JN331_002654"
     CDS             complement(4263..5561)
                     /gene="purA"
                     /locus_tag="JN331_002654"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY7260772.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5665..5862)
                     /locus_tag="JN331_002655"
     CDS             complement(5665..5862)
                     /locus_tag="JN331_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY7260773.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5944..6948)
                     /gene="hflC"
                     /locus_tag="JN331_002656"
     CDS             complement(5944..6948)
                     /gene="hflC"
                     /locus_tag="JN331_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY7260774.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWCISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6951..8210)
                     /gene="hflK"
                     /locus_tag="JN331_002657"
     CDS             complement(6951..8210)
                     /gene="hflK"
                     /locus_tag="JN331_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY7260775.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8296..9576)
                     /gene="hflX"
                     /locus_tag="JN331_002658"
     CDS             complement(8296..9576)
                     /gene="hflX"
                     /locus_tag="JN331_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY7260776.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9652..9960)
                     /gene="hfq"
                     /locus_tag="JN331_002659"
     CDS             complement(9652..9960)
                     /gene="hfq"
                     /locus_tag="JN331_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY7260777.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10046..10996)
                     /gene="miaA"
                     /locus_tag="JN331_002660"
     CDS             complement(10046..10996)
                     /gene="miaA"
                     /locus_tag="JN331_002660"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY7260778.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10989..12836)
                     /gene="mutL"
                     /locus_tag="JN331_002661"
     CDS             complement(10989..12836)
                     /gene="mutL"
                     /locus_tag="JN331_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY7260779.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIAPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSLAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12846..14183)
                     /gene="amiB"
                     /locus_tag="JN331_002662"
     CDS             complement(12846..14183)
                     /gene="amiB"
                     /locus_tag="JN331_002662"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY7260780.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRSEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQEQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14202..14663)
                     /gene="tsaE"
                     /locus_tag="JN331_002663"
     CDS             complement(14202..14663)
                     /gene="tsaE"
                     /locus_tag="JN331_002663"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY7260781.1"
                     /translation="MMNRVIPLPDEQVTLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14635..>15991)
                     /gene="nnr"
                     /locus_tag="JN331_002664"
     CDS             complement(14635..>15991)
                     /gene="nnr"
                     /locus_tag="JN331_002664"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7260782.1"
                     /translation="VLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLPEEAALA
                     REAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSHPAPIVA
                     VDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLGLDSWLA
                     GQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEAALRAGA
                     GLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQEWGKKAL
                     QKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIESDRLHCA
                     KRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGALLGQKL
                     SPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHDESSNSA
                     P"
BASE COUNT         3597 a         4605 c         3981 g         3808 t
ORIGIN      
        1 aatcgccata atcattcatt ttaataatta atgcgctttc gccatttgtt acatcaatat
       61 tttgttcagg tacagtttgc agcgccgtcg ccagcgccaa cggattccat gtcatatatt
      121 attccatata gattaagttt aaatattaat aaaatgaata tttgcaatac gtaattatct
      181 taccagctat agacaaaaaa aaaccatcca aatctggatg gcttttcata attcagagga
      241 actagctgcg ctgacgaacc gcttcaaata agcaaattcc ggttgcaacc gaaacgttca
      301 gggaagaaac acttcctgtc attgggatgc tgatcaactc atcgcaatgt tcacgggtca
      361 ggcgacgcat accttcacct tccgcgccca tcaccagcgc caggcgtccg gtcattttgc
      421 tctgatagag tgtatgatcc gcctcgcctg ccgtaccgac gatccagata ttctcttcct
      481 gcaacatacg catggtgcgc gcaaggttag tcacccgaat cagcggaacg ctttctgccg
      541 cgccgcaggc tacttttttc gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa
      601 tcaccgcatg aacgcctgcg gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat
      661 cggttacgcc gtcgaggatc agcaggaacg gttgatcgag cgaagcgatc agatccggca
      721 gatcgttttc ctgatactga cgtcctggct tcacgcgggc gataatgccc tgatgcacgg
      781 caccgtcgct tttctcgtcg agatattggc ggtttgccaa ctggataacc acgccctgag
      841 actcaagggc gtgaatcagc ggtaacagac gtttatcctc acggcctttt aaaataaaga
      901 cttcctgaaa acgttcaggg gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa
      961 tcatttcgct cattaatgta ctcgttgttt acgttttgcc tgatgcacta cgtttatccg
     1021 gcctacatga tctctgcaat atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt
     1081 cacgccgcat ccggcatgaa caaagcgcat tttgtcagca atctaaccct cttcttttaa
     1141 agagggtatt gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc
     1201 ggctattttc tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc
     1261 tttcggcttc gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt
     1321 accgacctga cgacgcttgc cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg
     1381 cgccgtttta ccgacgttgc gcggtgcgcg ttcactggag atcagactaa agtcgatttt
     1441 gcgctcgtcc atattaaccg cttcgacgcg aacttccacg cgatcgccca ggcgataagt
     1501 ctggccgctg gattccccca tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt
     1561 gtccagcgaa gagacatgga ccagaccatc aatgaacaag tcgtccagac ggacgaagaa
     1621 gccaaagcca gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat
     1681 gaagtcacac ttcagccagt cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc
     1741 catcgaacag tgctgaccca gttgcaacat ctcttccatc gaataatggt agccgccggt
     1801 ttcagtggtg ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg
     1861 cagcgtcagg tcaggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc
     1921 cagaccgaag tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag
     1981 catggtttgc agcatttctg catcaggacg gtcggcaacc gactccagca gttccgcgta
     2041 gtcacgcggt tccggcttgt taccacccgg cagctccagc cccagctccg ccagcactga
     2101 acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca gtgccggttc
     2161 tttcgctttc tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat
     2221 taatttgtgc gcgtcgttac gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt
     2281 gaaaatgaac ttcgcttctt cgctctcaaa tgagatccca ccgcgttctt cacgggcttt
     2341 atccagcact ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg
     2401 ctcgcgcaga tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc
     2461 gtgagagctc atcaccgctt cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac
     2521 cgtcatctcg cacaccatac acaggcggtc tacctgcggg ttgagcgaac acaggccgtt
     2581 agagagcact tccggcagca tcgggataac ctgcgaaggg aagtacaccg acgtgccacg
     2641 gttacgcgct tctctgtcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat
     2701 cgcgacccat aaacgccagc cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa
     2761 gtcacgggcg tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc
     2821 cgcttttgct tcttccggca cttcttcttt cagccccgca acctgttgct caacagcctg
     2881 cggccagata tacggaattt catgggtacg cagagcgata tcaaccgcca tgccggtgcc
     2941 catattgtcg cccagcactt cgacgatttt acccaccgct ttggtgcggc gagtcggacg
     3001 ctgagtcagt tcgactacga ccacaaagcc catccgcgcg cccatgatct gattgggcgg
     3061 gattaagata tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc
     3121 ggtaaagtag cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc
     3181 ttcacgacga cctttacggt cagcacccag cggctgcgcc agcacctgat cgccatgaat
     3241 gcaggttttc atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag
     3301 aaagccgtag ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg
     3361 cagcgcatag cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag
     3421 gcggcgacgc aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc
     3481 atcacggctg gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg
     3541 gatgggattc gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt
     3601 tcctccgttg tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc
     3661 ggttgattct cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt
     3721 tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca
     3781 ctgctgcaat tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc
     3841 gcactcgccg gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag
     3901 ccggcacgac taagttgatt gattattttg accatatgat tacgggagac gccgtagacg
     3961 tcagtcactt cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc
     4021 gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg
     4081 ggaaaaaata catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc
     4141 agttcgggga aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac
     4201 cgacacagca atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag
     4261 aattacgcgt cgaacgggtc gcgcagaatc atggtttcag tacgatccgg accggtagag
     4321 atgatatcga tcggcacacc ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc
     4381 tgcggcaggc cgctacgatc tttcacgccg aaagtggatt cagaccagcc cggcatggtt
     4441 tcgtaaatcg gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttcg
     4501 cgaccatccg gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg
     4561 tccagtttag tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca
     4621 acggtgtcca gccagccggt acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc
     4681 tgcttgcaga ggaactcgcc agtttcatca aacagttcgg ccgggaaagg acctgcacct
     4741 acacgagttg agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg
     4801 ccggaaccgg tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta
     4861 ccgtggtcga tatccagcag cgtaccctgc gcaccttcga acatgacgaa atcgccacgc
     4921 tgacgcgcct ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca
     4981 acagccatcg tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta
     5041 accaactgga agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg
     5101 tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg
     5161 ataccacgac cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc
     5221 agcgcaacgt gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga
     5281 acggggatgc cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc
     5341 acaacaccgt taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga
     5401 tggagaacgg ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg
     5461 tagcgtacaa catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg
     5521 tcaccccatt gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg
     5581 cttaaaaatg gattttacca tcgctttttc agagttacag cactttttgc actcaaaaat
     5641 gacctcgatc agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac
     5701 aacgcccgca accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt
     5761 catcgcagag atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag
     5821 taccaaaacc agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa
     5881 aagaaaacca ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt
     5941 atattaacgc gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct
     6001 catgaccatc acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat
     6061 gaatgcgtag aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc
     6121 atcgccttca ccacgcataa tgcggccctg acgctcagct tctgccagcg ttctggtcac
     6181 ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg
     6241 acgcgctacc gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt
     6301 cggcaggttg atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact
     6361 gttcgggttg atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc
     6421 ggcaatggcg ttatctgctg ccggggtagt aacttcatct tctgtacccg cagaaccgga
     6481 gttcagcgcg tcacgtactt cgagggtcag acgaccacgg gaatcggtga cgatatcttt
     6541 cacgtccagg cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac
     6601 ttccgcttgc gaaatgtcgc caccacccgt tgccaggtag taacggctga aatcgctgat
     6661 gcaccatttg atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc
     6721 ggcctggttg tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg
     6781 tatcttgaaa tgcagaccag gctcataaac caaaggtttg ttgtcatcgt cacgcagtac
     6841 cttaccaaaa cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta
     6901 aagcactacc agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc
     6961 tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga
     7021 ctggtggacg acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcagacgc
     7081 agcagattgc tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc
     7141 tggtctaacg gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg
     7201 ttacccaaca ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg
     7261 gctttatatt ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg
     7321 gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc
     7381 ggctgaactt cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc
     7441 gcggcaatcg catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag
     7501 ttgacgtcca gcagcgtgat acccatgtca tacggacgaa tcgtctcttc cagttcgcgc
     7561 tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta
     7621 ccgataactc cacgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc
     7681 acgctataca gatatttttc cggattggtg acgcggtact gcacgttcat ctcaacgcgc
     7741 actacgttct cgtccgacgt cagcatcaca ccagaagcgg ccagttcacg cacggcttcc
     7801 acgttcaccg gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc
     7861 agatggctga atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag
     7921 aaaccactgg ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg
     7981 ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg
     8041 ccgcccagac caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc
     8101 ccttgatcgc gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt
     8161 ccccacgggt cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc
     8221 catatttgtt atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct
     8281 tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg
     8341 acgccagtca acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca
     8401 ctctttttct attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg
     8461 cagacgcaat gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag
     8521 ctgtggtatc cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc
     8581 gtcccgatca atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag
     8641 ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc
     8701 ttgtacacgc acatccgccg catcaatgac gtgcagcagt aatgtggctt gccgcgtctc
     8761 ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc aggtggcgaa taaaccctac
     8821 ggtatctgca agtacggttt caccgacatc tgcaacgtca atacgccgca acgtcgggtc
     8881 gagggtggca aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag
     8941 ggtagattta ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat
     9001 acgcgattgc cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg
     9061 cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg
     9121 caaacctatc ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc
     9181 cagatggcgc agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg
     9241 ggcgaaaata tctaaaataa ggccggtgcg gtcgataaca cgacactcgc acaaacgctc
     9301 caggttacgc tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt
     9361 cgctttgaca gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg
     9421 cggcgcttta cggctaccgg taatcacctg caatgcttcg acaccggcgg aagagaccag
     9481 agattcaaac tcctggaggt cttccatatc tttgtcttgc gtaaaataga tgtgtaccag
     9541 taccgcctgc tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca
     9601 gcggggaacg caggatcgct ggctccccgt gtaaaaaaac agcccgaaac cttattcggt
     9661 ttcttcgctg tcctgttgcg cggaagtatt ctgcgcgctg ctaccatgat ggtagttact
     9721 gctggtaccg ccaccggcgt tgttactgtg atgagaaacc gggcgagacg ggacaacagt
     9781 agaaatcgcg tgcttgtaaa ccatctggct gaccgtgttt ttcaacagga tcacgaactg
     9841 atcaaaagac tcgatttgcc cttgcagctt aataccattc accaaataaa tagaaactgg
     9901 aacacgttcc cgacgcagtg cgttcaggaa cggatcttgt aaagattgcc ccttagccat
     9961 tctctctttt ccttatatgc ttatttgtac tttgaacctt tcgattctga aaaaattgcg
    10021 cacgatacgt ctcaattgta cacattcagc ctgcgatagc accaacaacc tgtaatactt
    10081 cgtcacgcgc ctgttctggt ttttcactgt caagccagtg aaccccttcc caaccacgca
    10141 gccaggttat ctgccgcttc gccaactgtc tcgtggcgca aacacctcga taaaccattt
    10201 catcgtatga gatttcgcct tcaaggtaag accacatctg gcgataaccc acgcaacgaa
    10261 tggaaggcaa gtccgtatgc aaatctcctc gggcaaaaag cgcccggact tctgcttcaa
    10321 aacctgaagc caacatctga tgaaaacgct gctcaatgcg ttgatggagc agttcacggc
    10381 tcgccggggc gatggcgaac tgatgcacct gatacggtag agcgtctcct gacgtttgcg
    10441 tcagttccgt taaagtttta cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt
    10501 gtggatcatt tggatgaatc cttgctgccg caaccggatc tacctcctga agttgacgat
    10561 gcaatgactc ccaaccttgc tctgccgcct gttgctcaat tctggcccgc acttccgggt
    10621 ctgccgacgg tagcggcgac aacccttcca gcaatgcctt gaaatacaac atcgtaccgc
    10681 ccactaacag tgggatccgc cccgccgcgg tgatatcggc catttccgcc agcgcatcgc
    10741 ggcgaaaatc agcagccgag taagcctgcg acggatcgcg aatatccagc aatcggtgcg
    10801 gcgcggcgag taactcttca gcgttcggct tcgccgtccc gatatccatc cctttgtaaa
    10861 taagggcaga atcaacgctt atcaactcta ctggtaaaat tttacgcagc tcaatggcta
    10921 acgccgtttt accggaggcc gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct
    10981 tactgatatc actcatcttt cagggctttt atcgccggat gtaaatcaac agattgtaac
    11041 agaccacccg gcggcgtttt cacaagttgc ggacataacc gttccacgtc cgccagcagg
    11101 gttatggcct gtgccattga ccactgcgca tgttcgctca tcagatttcg tgcaatccac
    11161 tgcgcaatat tgccaggttc gaatacggac tgcttcgcca ggtagcctat cagttcagga
    11221 atcaagattt gtaaattttg ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc
    11281 tgtgcatctg actggaaatc aatacccaat tccgctaggg cagactgcgc tttttctaat
    11341 gccgattttt cttcgccaga aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa
    11401 acgggcgctt cacccggcgt caattgtgcc tgacgcagcc aacgttctgc cactggcaag
    11461 gataaaagtg aaatgttgcc atcgcgctcc agcaacgcac agtcggaatg gacgatagtc
    11521 agtacccgac caaaactctg actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct
    11581 tttggttttt gcatcggcgc gggcgtttgc aaaagctggc gatacacttc accttgctgt
    11641 ttctggtagc ctggctgcgc attcggccag ggggcagccg gacgactgcc tgaggcgggc
    11701 gcaagagagt agcgcggagc taccggctca cgagctgccg gttctgcaaa gtgattgcgc
    11761 cccgccgcca cgcggttttc cgggatggca cgcggtgcgg gttgtggttc atcgtccagc
    11821 ggtagcggcg tttccagttg ctgttgtagc acgctcagca cgccctgata gataaaatca
    11881 tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc
    11941 acctgatgcg gggcgatctc cagatacaac acaaatgccg gttgctgatc ggcccccagt
    12001 ttgtcttcgc aggcctggcg gatcgcatga ttgatcaggc gatcgcgcat catgcgacca
    12061 ttcacgtagc aatactgaat ttccgccagt gcgggcgtag tgtgatttgg atcggccacc
    12121 cagccgcgca gggtgagatc gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa
    12181 gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc cgccttccgg cactgcgcgg
    12241 tactgacgca caattttacc gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc
    12301 gcaatgcggc ggatgatttc atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat
    12361 ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct ccagcgtcgt tcccaccgga
    12421 tgcgccgccg gtttaaccgt cacgtccatg tcgcgtcctt cggcataggc ctgccaggct
    12481 tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc
    12541 agcgcctcac cgcgaaagcc caggctgata atggcttcga gatcgtccag agaggcgatt
    12601 ttactggtgg catgacgagc cagcgccagc gccagctcat cttttttgat accgcagccg
    12661 ttatcacgaa tgcggataag tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc
    12721 gcacctgcat cgaggctgtt ttccactagt tctttgacta ccgacgcagg tcgctcgacc
    12781 acctcacctg cggcaatctg gttcgccagt tgtggcggta agacctgaat tggcatcaat
    12841 cgtccttagt ttggcagcgt gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt
    12901 gcaccctgcg gcgcagattg catcggatgc gcaaggaagt aattgcgcag gcctttgtaa
    12961 atggcttctg ccagctgttc ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg
    13021 ttgctgataa aaccggtttc gaccagtact gatgggatat ccggcgagcg cagaacgcca
    13081 aggctggcgt gttctgatcg acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc
    13141 atactggtcg ctacatcata ccctacccgc tgggaatgac cgaactgtaa atccagcacc
    13201 gcctggctta aataggggtc agactgactg ttcgccagca catcacccgc cccacccagc
    13261 agctccgact gtttctcgtg ctgctccagc cagctggcca tttcactgtt ggcgcgacgg
    13321 ttagagagca cccatacgga agcgccagtc gcactgcggt tcggtgcggc atcagcgtga
    13381 atcgacacga ggaaattggc gttttgctta cgtgccacat cactgcgccc catcaccgag
    13441 ataaagtaat ccccgtcacg ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa
    13501 gtacgcaatt tacgcgcgat ggcgatggtg acatttttct cccgcgtacc accggggccg
    13561 atagcgccag ggtcctgacc gccgtgtccg gcatcaatag cgatgataat tttatcgcca
    13621 gtgttagccg tcgcgcgcgc tgccggacgc gttaccgtat tactgctgat aacacccgta
    13681 gtgcggttac tttccgtttt aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca
    13741 accgcaggcg tttcaacgcg tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca
    13801 tcggcgttaa tcgtaaagac gacagtgtaa ttgctgccat tctgccgctt caccgcttcg
    13861 gttttaccgt tttcggtaag atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt
    13921 ccagagcgaa tcgccttcac cagattattg ccgctgaaca acaacggcag tccctgaatc
    13981 acgcccgttt gtttgatatc gagcgccacg gtgcgtttgc tttgatggct aaacgcataa
    14041 tcaggatcgc caataaaact caacgttatc cgcgcctgtt gattaccgtt agaaacctga
    14101 atatcagaga gcgtcgcggc acccaccggc gtgcacagca gcagcagcgt cgctaccaac
    14161 caatttctga tgcgatacat catcccgcca cctttcaaag gttaaccggc taaacgcgcc
    14221 agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga
    14281 taatcaatgt gtatttcgac atccgggtca ggaagaacac ctgtaccttg ttgtggccac
    14341 tccaccaggc agatggcatc gttggcaaaa taatcgcgga tccccataaa ctccagctcc
    14401 tcgggatcgg caaggcggta caaatcaaag tgatagacca ttaagttgtc gagcgtatag
    14461 ggttcgacca gcgtataagt ggggcttttg acattaccct gatgacccag agcctgtaaa
    14521 aagccccggc taaaggtggt tttacctgcg cctaaatcgc catacagata gattacggtt
    14581 gcgccatcgc aggctttcgc tacccgctcg cccaggtcta atgttacctg ctcatcaggg
    14641 agcggaatta ctcgattcat catggttttt atcagtcact tccgggttaa caatacgctg
    14701 tagcgtggaa aagagatcgg ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac
    14761 gtcagctgcc gcaccgtgcg cgacacagcc tgcacaggct gcatcatacg gcgacagttt
    14821 ttgcccaagc aatgcgccaa taataccaga gagcacatcg cccatgccgc cgctcgccat
    14881 gcctgcattt ccggcatcaa taatgcctaa agcgtcagga tgggcggcga ccacggttcc
    14941 ggcacctttc agcaccgcta cgccgccata acgttgtacc agacgtttgg cgcaatgtaa
    15001 gcggtcactt tcaatttcag cgacggaaca gcctaacaac cgtgcggcct cgccaggatg
    15061 cggcgtgatc acgcgattgt gacgcttatc gggattgatt gccagcaggt tcaatgcatc
    15121 ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt tgcagggctt ttttccccca
    15181 ctcttgctgg cccagaccgg gaccaatcac caccacatcg gcccattcca ggctttcggt
    15241 aagagagtcc atagtcagtt catgcaccat caattctggt cgtgcagtca gcagcggcgc
    15301 aatgttctca ctgcgggtca gtactcggac taaaccagca ccagcacgta gcgccgcttc
    15361 ccccgtcata cgaatagccc ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc
    15421 gtgatcgcct ttatgcgaag tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc
    15481 cgaaaaccgc tgaattttcg tctcctgacc tgccagccag ctatccagcc ccagtgagtc
    15541 aaaatgcagt tgtcctgtaa catcccgcgc ttttccagtg agcaagccta gtttcagcgc
    15601 aataaaagtg atggtgtgat cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc
    15661 cagcaggccg gaagggatat caaccgccac aatcggcgca ggatgggaat tagcgtggtc
    15721 gattaactgg ctaatggatt cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc
    15781 aacaatcaga tctaccgatt cgggccagac aatattcgaa gcatggatct cgccacccgc
    15841 gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc aacggtttgt cgctctcctg
    15901 ggccaacaac gtgacctcaa tgccgaccgc tttggccagt cgcgcgacca cgtagccatc
    15961 gccgccgtta ttaccatgac cgcacagcac c
//
LOCUS       DADLSB010000090        15867 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.090, whole genome shotgun sequence.
ACCESSION   DADLSB010000090 DADLSB010000000
VERSION     DADLSB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.090"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..882
                     /locus_tag="JN331_002665"
     CDS             <1..882
                     /locus_tag="JN331_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7260783.1"
                     /translation="RNYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFST
                     FLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVV
                     TQPVEILPSTHRKRDTRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGI
                     AAGLEIPMMLIAGYYMKRIDKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIF
                     LGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLA
                     IGMLGIAMICLLFIKDI"
     gene            993..1916
                     /locus_tag="JN331_002666"
     CDS             993..1916
                     /locus_tag="JN331_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7260784.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1920..2738)
                     /gene="nlpA"
                     /locus_tag="JN331_002667"
     CDS             complement(1920..2738)
                     /gene="nlpA"
                     /locus_tag="JN331_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY7260785.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAQEDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2960..3252
                     /locus_tag="JN331_002668"
                     /pseudo
     CDS             2960..3252
                     /locus_tag="JN331_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3293..4483)
                     /gene="nepI"
                     /locus_tag="JN331_002669"
     CDS             complement(3293..4483)
                     /gene="nepI"
                     /locus_tag="JN331_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY7260786.1"
                     /translation="MSEFIAENCGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNIFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIGSFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4694..5146)
                     /locus_tag="JN331_002670"
     CDS             complement(4694..5146)
                     /locus_tag="JN331_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY7260787.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5199..6533)
                     /locus_tag="JN331_002671"
     CDS             complement(5199..6533)
                     /locus_tag="JN331_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7260788.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6708..8460
                     /gene="adeD"
                     /locus_tag="JN331_002672"
                     /pseudo
     CDS             6708..8460
                     /gene="adeD"
                     /locus_tag="JN331_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
     gene            complement(8506..9897)
                     /gene="uhpT"
                     /locus_tag="JN331_002673"
     CDS             complement(8506..9897)
                     /gene="uhpT"
                     /locus_tag="JN331_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY7260789.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10035..11354)
                     /gene="uhpC"
                     /locus_tag="JN331_002674"
     CDS             complement(10035..11354)
                     /gene="uhpC"
                     /locus_tag="JN331_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY7260790.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(11364..12866)
                     /gene="uhpB"
                     /locus_tag="JN331_002675"
     CDS             complement(11364..12866)
                     /gene="uhpB"
                     /locus_tag="JN331_002675"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY7260791.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12866..13456)
                     /gene="uhpA"
                     /locus_tag="JN331_002676"
     CDS             complement(12866..13456)
                     /gene="uhpA"
                     /locus_tag="JN331_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY7260792.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13531..13821)
                     /locus_tag="JN331_002677"
     CDS             complement(13531..13821)
                     /locus_tag="JN331_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY7260793.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFSRRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13825..15513)
                     /gene="ilvB"
                     /locus_tag="JN331_002678"
     CDS             complement(13825..15513)
                     /gene="ilvB"
                     /locus_tag="JN331_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY7260794.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(15619..15717)
                     /gene="ivbL"
                     /locus_tag="JN331_002679"
     CDS             complement(15619..15717)
                     /gene="ivbL"
                     /locus_tag="JN331_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY7260795.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
BASE COUNT         4086 a         4311 c         3910 g         3560 t
ORIGIN      
        1 cgcaactact tcattctcct ctctacgggc gtacttctga gtagttttgc ttctaccgca
       61 aacccgcaaa tgttcgccct cgcccgtgaa cacgccgaca gaacaggccg tgagacggtc
      121 atgttcagta catttttacg tgctcaaatc tcgcttgcct gggttatcgg gccaccgctc
      181 gcttatgaac tggcaatggg gtttagtttt aaagtgatgt atctcaccgc tgccatcgca
      241 tttgttgttt gcgggctgat agtctggttg tttttgccat caatacaaag aaatattcct
      301 gtcgttaccc aacccgtaga aattttaccc tccacccata ggaagcggga tacgcggcta
      361 ctttttgtgg tctgttcaat gatgtgggcg gcgaataatc tctacatgat aaatatgccg
      421 ctatttatta ttgatgaact gcatctaacc gataaactgg ctggagaaat gattggtatc
      481 gctgccggtc tggaaattcc gatgatgtta atcgcaggct attacatgaa acgtattgac
      541 aagcgactat taatgctcat tgctatcgtg agtggtatgt gtttttacgc cagcgtactc
      601 atggcgacga ctccggcggt tgagctggaa ttgcaaattc taaatgccat cttccttggt
      661 attctctgtg gtatcggcat gctttatttt caggacttga tgcctgaaaa aataggctct
      721 gcgacaacgt tatatgcaaa tacttcacgc gtcggctgga ttatcgccgg ctctgttgac
      781 ggaattatgg ttgaaatctg gagctaccat gcgttgttct ggctggcgat agggatgttg
      841 ggtattgcga tgatttgcct gctgtttatt aaagatattt agctcaccag tctgggctaa
      901 gaaaaatcgg cgtggcgcaa acagagccat cctcgccatt tttgctatca tgcctgcata
      961 cataaacgac aaaacagtat gcagagggaa aaatgggttc caccagaaag gggatgctga
     1021 acgttctgat tgccgccgtg ttgtggggaa gttcaggggt ctgtgcgcaa tacatcatgg
     1081 agcaaagcca gatgtcgtcg cagtttttga ctatgacgcg tttgatattc gccggtttga
     1141 ttctgctgac gctgtcattt gttcatggcg ataaaatctt ttctattatt aacaatcaca
     1201 aagatgccat tagtctgctg attttttccg tggttggcgc gctaaccgta cagctcactt
     1261 ttttgctaac catcgaaaaa tcgaacgcag ccacggcaac ggtgctgcaa ttcctctcac
     1321 cgacgattat cgtcgcctgg ttctcactgg tacgtaaatc gcgcccgggc attctggttt
     1381 tctgcgctat tttgacatcg ctggtcggga cttttttatt ggtgacacac ggtaatccga
     1441 cgtcattatc gatctctcct gccgcgttat tctggggcat tgcctcggca tttgctgctg
     1501 cattctatac cacctatccc tcaacgctga ttgcccgcta tggcacgttg cctgtcgtcg
     1561 gctggagtat gctgatcggc ggtctgattc tgttgccttt ttatgccaga caagggacaa
     1621 actttgtcgt taacggcagt ttgattttgg cgttttttta tttgatcgtc attggtacgt
     1681 ccctgacatt tagtctgtac ctgaaaggag cacaattaat tggcggtcca aaagccagca
     1741 ttttgagctg tgcagaacca ttaagtagcg cgctactctc tttgctgttg ctggggatca
     1801 cgttcacatt accggactgg ctgggaacgc tgctgattct gtcatcggta attttgattt
     1861 caatggattc ccgtcgccgc gccagaaaaa taaatcgtcc ggcgcgggat gagtgagaat
     1921 taccagccag gcaccgcgcc accgttaaag atggtttctg ccgctttagc gacttcggat
     1981 gattgataag attgcagaaa ttccttcacg ttttctgcat tcttattatc ttcctgtgcc
     2041 accaaaatat tcacatacgg cgaattttta tcttcaataa atacgctgtc gtgcaccgga
     2101 gaaagcccgg tctgctgaat gtaagtggtg ctgataatcg ccacatcaac tttgggatca
     2161 tccagtacgc gcgggagctg cgccccctcg agttccataa tctgcaaatg acgaggatta
     2221 tcggttatat ccagcgcggt aggtaataac ccttttccct ctttcagagt aattagcttc
     2281 tctttttgca acagtaaaag cgcacgacca aggttggtcg ggtcgttggg gatcgccact
     2341 gtcgcgcctt cttttatttg cgccacggtt ttgatttttt tggaataacc cgccatgggg
     2401 aagacaaacg tatttcccac cgctaccagt ttataaccat gcgcctgatt atcttgttca
     2461 agaaagggtc gatgctggaa gacgttcgca tcaagttcgc catgatttgt cgcatcgttc
     2521 ggtagcaacg aaccgctaaa tcctaccagt tgaacatcga ggccatattt ctcttttgcc
     2581 acctttttag cgacttccgc gacatcttgt tctgcgccat ttataacgcc aactttaatg
     2641 tgttttgcat cgctgctact ctggtcgcaa cctgccagca gaattccggc cagcaataat
     2701 gcggcccctg tccgtagatg atgtgttgtc agtttcattt ttttatcctt ttattaatga
     2761 ttacctgact atagcggtaa ggtcgctgcg gtttaaaaaa cgaaacgcta tcgataagaa
     2821 taaaaaggaa taaaagtgga atataaagtc tggcacttct tactgaccac gcaagcgcgt
     2881 ttcgtacagc atgatgagag cgatgaaagc aaactacact tatgcttcat tcgatacaca
     2941 tttgtaaaag gttgaatcca tgaaaccaac gacactactg cttattttca cttttttcgc
     3001 tatgccaggc atcgtatacg ccgagtcgcc ttttagctca ctgcagtccg caaaagagaa
     3061 aaccacggta ttacaagatt tacgtaaaat ttgcacgcca caggcgtcat tatcagatga
     3121 agcgtgggaa aagttaatgt tgtcagatga aaacaataaa caacatatac gcgaggccat
     3181 cgtggcgatg gaacgtaata atcagagcaa ttattgggaa gcgttgggta ggtagaatgc
     3241 ccggatatgt aagaacagca acaactactg accaacgctt ctttacctga tatcaggatt
     3301 tcttcatttt cacctttgca gtaaccaaca atgcagtcag caacatcaat gtgccggaca
     3361 acatcaacgg cgaagtcaga ccaatattat ccagcgcata accgccgatt gccgcgccac
     3421 aggtattagc aagctgaata accgccacct gaatagaccc ggctttttct gcctgatcgg
     3481 ccagcgagcg ggtgatccac gttgaccagc cgacgggaac caatgcaaaa gttagccccc
     3541 agataatcgc cacgccggta gcaacgattt tatcgcttcc ccacaacgtc agtaccaacg
     3601 cactcacagc cagtattaac ggcgcgcctg ctaaggccag ttttaccgaa cgtttaagaa
     3661 tgaacgacga aagcgacgta ccaataaagc tgccgatacc aaaactcaac agcaccagcg
     3721 ttaagccatc cacgccgaat cccgctaggt tcatatacac cgggcgaata tacgtgaaga
     3781 aagcaaactg cccggcgaaa gacatgaaga tggcgatcat ccctgccatc acacccggac
     3841 gttgtaataa gcggaaaata ttttgtttct gatgcgaggg ttcgcctggc agtgaaggca
     3901 atgatttgat aatccagaaa atacacagca cgcccatcac cgccgccgca ttaaagacat
     3961 tgcgccaacc gataagctcg cctaaaaaac tgcccaacgg cgcggcaatc accagcgcaa
     4021 tagaaaccgc gccgaagatc accgacagcg ccttcggcac cgtacgcggc ggtaccagac
     4081 gcatggtcag cgacgccgac atcgcccaga acccgcccag cgccagcccc agacaggcac
     4141 gaccgattaa aagcaaactg aatgagttag caaaggaaac cagcaagcag gagagcgtca
     4201 gcaaaacggc aaacaaaata acaacgtagc ggcggtcagt ggcctgaatt gtctgggtaa
     4261 taaacaaact ggcaaacatt gccacaaagg cggtcacggt caccgattgc ccggcaaccc
     4321 cttccgaaat gcctaaatcc tgggccattg gcgtcaacaa actgacgggc aaaaactcaa
     4381 cgataatcag acaggcgaca caaaacgcca ccgagaaaac ggctgaccag ttcggtcggg
     4441 tgatggcatc cgcgccgcag ttttcggcaa taaattcact catggtgtta cccgtggcat
     4501 gtttcagggg aaaagccgac cactttaaca ttgaaatgtg tgacgcattt aacgtttttg
     4561 caacttttac ggtgttgctt gagctggagt taagatcgaa acggaacagg gcaaagatta
     4621 ccggatgcgg cgtgaacgtc ttatccaccc gacaggtcag gcgcggtctg taggctgata
     4681 agacgaaacg gtttcaatgc aaccagtgaa cgccctcggc aggctgaaag aacgcgttat
     4741 agcgcatctg aaaagcatta atatcctgca tttcaggttc ggtttcacgc agaaaaccca
     4801 gcgccagcct gacctgagca tcggtaatcg gcgcggaaag gcgggctttt tgtaaaatcc
     4861 ggtgccattg cagcaggttc tgttcgctat catcgacaat gacaatttgc cagatgagca
     4921 acacctgcgc ggcgttttgc aggtgcgact catccgggcg atgcaaataa cgtaaaaatt
     4981 cgtcaccggc tgactttccc atttgatcga ccatcgactc cacgggtacg acatcgatat
     5041 tcagccgatc gctgagacgg cgaatcgctt ttctggcccc ggatgtcagc acccgaaaac
     5101 caaccacaaa cactaccgcc agcgtggcga gcattatcca gatcatgagt tctcctgcgt
     5161 aatcagaact catgatatct ggaaaccctc gccagttact aatccaccag aataatcttc
     5221 aatgcgaaca gagctgcgac caccaccaca cacaggttca gatcgcgcca gcgcccggtg
     5281 cacactttca tgatgcagta cgacataaag ccgagtgcaa tcccttcggt gatcgagaaa
     5341 gtaaagggca tcatcaccgt ggtaataaac gcaggcaccg attcggtaaa atcatcccag
     5401 ttaacgcgcg ccaggctcga agtcatcagc acgccaacaa agattaacgc tccagcggtt
     5461 gcgtaaggag gaaccatcgc caccagcggt gagaagaaca taactaacag gaacataacg
     5521 ccgaccacga cagccgttag ccccgtgcga ccaccgactg ccacaccaga agtactttca
     5581 atataggcag taacagacga ggtaccgata aatgcgcctg ccaccgaact gacgctatca
     5641 acatacagcg ccttattcat attggggaat ttaccgttgc tatctattaa gcccgcttta
     5701 tcagttacac caattaatgt tcctgatgaa tcaaataggt tgatcagcat aaaggagaaa
     5761 atgataccgg cgagttcaag tgttaacgcg ccgctcaaat ctacttcacc aatgacgccg
     5821 ctaatatcag gcggaatgga atagacgccg ctaaaatgaa catcaccgaa aaataatcca
     5881 cagcaagacg tcaccacaat agaaaccagc accgcggcat gaaaatgacg tgatgacaac
     5941 acggttataa taaaaaaccc taaaatacct aacaacacgc cgtgagaact taaatcgcca
     6001 atcatcacca gcgtgtcttt attggcgaca ataacaccag tgttttttaa tcccattaag
     6061 gcaataaata atccgattcc actggtgata ccaatacgta aacttaatgg aatgttggag
     6121 atcatccagt accggatacg aaacagcgtg agcaaaaata gcccaattgc gccccagaat
     6181 atcgcgccca tcccggtctg ccaggagatc cccatcgccc ccacgaccac gaacgcaaag
     6241 aaggcgttca gccccattgc cggagccagc gccacgggta agttagcaaa tatccccatc
     6301 gcaatactgc cgataccggc aatcaaacag gtggtaacaa acaccacttt cgggtccatt
     6361 tgtgccgcgc cgaggatttg cgggttcaca aaaacaatgt acaccatggt taaaaaagtg
     6421 gtcatccccg caatcaattc tgtgcggacg gtggtcccat gctgaggtag tttaaataat
     6481 cgcgaaagcg tccctgattc attactcacg taatcggtat tgtcattatt catttttttg
     6541 tccattggag gagatttaat cccttgcttt tgtaaaacaa atgacaacaa cggttcagtg
     6601 ataattatca catttcaatt gcacattaat agatattctt taataatctc gcgacgtttc
     6661 tttatgataa ataataatca aattgataaa atcaaaatga gaaaaatatg aataattcca
     6721 ttaaccataa atttcatcac attagccggg ctgaatacca ggaattgtta gccgtttccc
     6781 gtggcgacgc tgttgccgat tatattattg ataatgtctc tattctcgac ctgatcaatg
     6841 ccggagaaat ttccggccca attgtgatta aaggacgtta cattgccggt gttggcgcag
     6901 aatacgctga tgctccggct ttgcagcgga ttgatgctcg cggcgcaacg gcggtgccag
     6961 ggtttattga tgctcacctg catattgaat ccagcatgat gacgccggtc acttttgaaa
     7021 ccgctaccct gccgcgcggc ctgacgaccg ttatttgcga ccctcatgaa atcgtcaacg
     7081 tgatgggaga agccggattc gcctggtttg cccgctgtgc cgaacaggca aggcaaaacc
     7141 agtacttaca ggtcagctct tgcgtgcccg ctctggaagg ctgcgatgtt aacggtgcca
     7201 gttttaccct tgaacagatg ctcgcctggc gggaccatcc gcaggttacc ggccttgcag
     7261 aaatgatgga ctaccctggc gtaattaacg ggcagaatgc gctgctcgat aaactcgatg
     7321 catttcgcta cctgacgctg gacggtcact gtccgggttt gggtggcaaa gaacttaacg
     7381 cctatattgc tgcgggtatt gaaaactgcc acgaaagtta tcagctggaa gaaggacgcc
     7441 ggaaattaca actcggcatg tcgttgatga tccgcgaagg gtccgctgcc cgcaatctca
     7501 acgcactggc accgttgatc aacgaattta acagcccgca atgcatgctc tgtaccgatg
     7561 accgtaaccc gtgggagatc gcccatgaag ggcatatcga tgccttaatt cgccgcctga
     7621 tcgaacaaca caatgtgccg ctgcatgtgg catatcgcgt cgccagctgg tcgacggcgc
     7681 gccactttgg tctgaatcac ctcggcttac tggcacccgg caagcaggcc gatatcgtcc
     7741 tgttgagcga tgcgcgtaag gtcacggtgc agcaggtact ggtgaaaggc gaaccgattg
     7801 atgcgcaaac cttacaggcg gaagagtcgg cgagactggc gctgtctgcc ccgccatatg
     7861 gcaataccat tgcccgccag cgactttgcc ctgcaattca cgcccggaaa acgctatcgg
     7921 gtcattgacg tcatccataa cgaattgatt acgcactccc gctccagcgt ctacagcgaa
     7981 aatggttttg atcgcgatga tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg
     8041 ctggctccgg cctgtggttt gctcggcggc tttggcctga atgaaggcgc gctggctgcg
     8101 acagtcagcc atgacagcca taatattgtg gtgatcggtc gcagtgccga agagatggcg
     8161 ctggcggtca atcaggtgat tcaggatggc ggcgggctgt gcgtggtacg taacggccag
     8221 gtacaaagtc atctgccgtt acccattgcc gggctgatga gcaccgacac ggcgcagtcg
     8281 ctggcggaac aaattgacgc cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat
     8341 gagccgttta ttcagatggc gtttctttct ctgccagtga tccccgcgct aaaactaacc
     8401 agtcaggggc tatttgatgg cgagaagttt gccttcacta cgctggaagt cacggaataa
     8461 taaaaaaagc ccggcgtcat gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa
     8521 ctgctgaatt tttttctcgc ggcggatttt gcgttcttcc attaccgcca ctatcgccat
     8581 caggcagata caaccaatcg cggcgatatc cagcgcggcg aaggtgcctg cccagccggt
     8641 aaggccgaat accggcgtcc catcggcaat cattcccaga cctaacttgg caaagctgtc
     8701 accaatcagg taagcaaagg tgcctttaat accatcggca gcgccaatcg cttttttagg
     8761 tacaaagcca acagcagcca caccaatcaa caattgcggg ccaaagacca ggaaacccaa
     8821 cgcaaagaga gaagccagat agatatattc gttactggca tgttgataca caccgagcgt
     8881 ggcgataatc agcgccagcg cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc
     8941 agagagccag ccccacagca gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc
     9001 ctgaatcgcc accgctttag agagtttcag ttcctggaac gcgtatacgg ttgaccactg
     9061 gtcgatacca atacgtacca catagaggaa aatgttggcg aagcacagca gccagatcac
     9121 tttgtttttc agcacatact caacaaagat ctgccacttg gtcatatcgg tagattctgt
     9181 ctctttgtcc tcttcgctga tctcctcgcc gaacagttct tcagctttgc cgaggccata
     9241 agattccggg gagtcgctgc cgtaacgcag gccgataaaa ccgacaatca gcgcgataat
     9301 cgacgggaag ataaacatgc cgatgacatg gccatcgaac aggtaatttg ccccgaacag
     9361 cgccacacct gctgcgcctg caccgccaag attgtgagaa atattccaga aaccgaggaa
     9421 tgtcccgcgt ttacgacgcg gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc
     9481 ggtactctgg aaaaagccgc ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga
     9541 gccgctgccc atactggcac tgaagcccag catacaaatg gcagagagga taagcatgaa
     9601 cggcaggaat tgtttggtgt ttttgccgtc ggcgtagtat gaaaccaacg ttttacccac
     9661 gccataagtg atggagaaac ccaggccgat catccccagc tgcgtcatgc tcaacccgta
     9721 ggtcgaaatc atatcgttct gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag
     9781 gtagccgata aagaccacca ggtaggattg catgaacggt ttgaaccaca ttttgcgccg
     9841 cacttcgagc ggaaggtcca gggtcggctt gcgaacctgg tttaggaaag ccagcatggg
     9901 ttactcctga aatgaatacc tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg
     9961 cctgagatag cgtccaagaa aaacctggaa aatttcttag ttttgccgga tatgaagtga
    10021 aaaggtgaga tgcatcacgc ttcacgcggt gtctgggcgt tcaaaaaggg cagtaacagc
    10081 agtgcggaaa tcccggcggc gatagagata accacaaaaa atccgctcca gtgccaggta
    10141 tcgagcactt tcgccagcgg ccaaccagca agcgacgccc ccagataagc aaacaagccg
    10201 acaaaccccg tcgccgcccc tgccgcctct ttgtgggaac actctgccgc cgccatacca
    10261 attaacatct gcgggccaaa gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc
    10321 acgtagctgg caaatggcat cagccacagc gagccgactg aaagcaaaat tccggcggcg
    10381 aaaatcaaat tcatcggccc acggttgccg ttaaacaatt tgtccgagcc ccaaccggct
    10441 accagcgcac cgataaatcc gcccagttca aacatcgtca ctgccgtatt cgccgtgacc
    10501 agatcgacgc ccagcgtctc ggacatatac aaattgcccc agtcgttgat cgccgcccgg
    10561 accacataga ccagcacata gcaaaacgaa agcagccaga tatacggatt cagcaacaca
    10621 tatttggtga ggatctcttt acgcgtcaac cctgcccctt cttgttgttg agcaatttcc
    10681 agcgcgtcgt gccgccattc accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgt
    10741 agccgccagc agagaaaaat ccccacgact atcgccatac aaccagcaat catcatcccg
    10801 gcacgccagc cgtaatgcag cgcgctcgct gccatcacaa tgggaatgag tgcgccgccg
    10861 acgttatgcg ccgtgttcca taatgcccac caaccgccgc gctcggtacg tgaataccag
    10921 gccgttaaca gacgcgcaca caccggtgaa ccccagccct ggaaaaaggc gttcagcacc
    10981 cagagtacgg caaacgccca tagcgacgtc gagaagccaa acagaatgtt catgatgccc
    11041 gtggcaataa gccctatccc cataaaataa cgggcatttg agcgatcgct gacaatgccg
    11101 gagacaaact tcgacacgcc gtaagtaatg taaaacaggg tcgctaacag gccgatatcg
    11161 ctacggctga gcacgccgtt agcaaggatt tctggtaccg cggcgttaaa acttttccgc
    11221 gtgaagtaaa acagcgcgta acccagccag atggtcagca gaatatgccg acgccagtag
    11281 cgataacggg catcaatttc atgtttatca gtcattaatg gcgcatcggc aggcgctttc
    11341 agaaacggca acatcatcga accttagaca taacgttgag gtagagaaac gctgacacgc
    11401 gtgccgtgca gacaggaaat gtgtaatgtg ccgcccagcg ccgttacgcg ctcgcgcatt
    11461 ccggtgaggc caaaaccttg ttgcccggaa cccggcggca aaccgctgcc atcgtcttca
    11521 atcaccagca tcaaccgttc atcctgctgc cagccttgca gcgtaaccgc gctggcatcg
    11581 gcatgtttca caatgttgtt cagcccttcc tggcagacac gaaacagcgt cacgcgctgg
    11641 ttttcgctta atgctgattc atcgatacgc cattcgagat ggctgacaat gccgcgccct
    11701 tccagctcca tttcccgcat cagtgagcgg atggcctgct ccagggtgag atcatccagc
    11761 tggcgcggac gtaaccgtcc taacaaacgg cgcaccgcgt cgtaaacgcc cagcgacagt
    11821 tgttcgatga gctgcccgct ctgcttcaca ctggcgttat ctgccgccag ccgctgaaca
    11881 atgcccgcct gagtacgaat agcagtgatg gtctgaccga tatcgtcgtg cagctcacgc
    11941 gccacgtcgc ggcgcacgct ctcttcggtt tctaacaaac gttcagccag atgctgattg
    12001 cgcgccagtt ccttttgtag cgactggtta agttcacgca accgctggat gcctgcgccc
    12061 agtaacaacc ctgtcagact ttgcaccagc aacgagagta ataaatccac cggatgatcg
    12121 cgccaggttt gactggcgat cagcgcgatg gcgttcatca acgtcgcaat cagcgctcct
    12181 tgccagccat agtgccaggc cagcgcgata atcggcagcg ccagacagaa tggcgtaaag
    12241 cgcgacagtt cgtccggcaa tcccaactgg agccagagac tgataacaaa cagcagcaag
    12301 taccagacca gatgccgccc gcgccagttg atgggctgag aaaccagtga cgggccgagc
    12361 ggcagccagg tgttattggc gagatagtgc cagaacacca gacatatcgg ggccagcgtc
    12421 agaccgccag ttaaagtcag caacagcgca ttccacgact ctttgccgtg ccaaagccag
    12481 ggcagcgact gcaacaacgc cgccgccgtt aacgccgcac cctgtaacag caaggtgcgc
    12541 cagtcacgct gatggcgata gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta
    12601 ccgatcatca ataacggaaa atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc
    12661 agccactccg cacccagcaa tacgggccag tatccgcgcg ggcattgcag cattagcccc
    12721 agacgcagac caaacggaaa taacagcacc gccatatcag ggcgctcaac cagatgcagg
    12781 ctgatactcc ataggcaaaa ccatgcggca gagaagataa aaaagcaggc aataacggta
    12841 attaagcggg agaacaacgt cttcatcacc agccatcaaa catgcggcgc gccagctcta
    12901 cgtcgttact gacgcccagt ttttccatca gattggcgcg atggacgtgt accgttttcg
    12961 gtgacaagcc cagttcggcg gcaatctctt tcaccgccat tccttgcgcc agtttttccg
    13021 ccacctggcg ttcacgtttg gtcagcgggt cctgacgacc ggatgccagt ttaatggcaa
    13081 tatccggcgt cagataacag ccgcccgtgg caaccgtatg caccgcagca atgagttcat
    13141 ccgggctaca gcgtttggaa agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca
    13201 gcgcaggact gtcgtgaacg gagagcataa tcgtcgccat acctttcggc agctggctta
    13261 gcagctccag accggagata tcgggcatgg agatatcgca aatacacacc tgcacaccgc
    13321 gccccggcag ccccgccagc gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat
    13381 caggttccag ccccagcagc tgcgcaaagc cggagcggac gatgaggtga tcgtctataa
    13441 gggcaacggt gatcatggtc ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga
    13501 taagcgcgat gttgttcaag ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa
    13561 catcgtcgga tcggactgat tacgctgcac tttcacgaca tcttccagct tatcgatttg
    13621 gcttatcatc tgctccagac gctggtcgtc attgaccagt agccagatat ggcttttgtc
    13681 gctgtcctga atcggcagac aaagaatgcc ttcaacgtta aaagcgcggc gggaaaaaag
    13741 gccacaaacg tgggtcatta cgcccggatg gttgcgaacg gtgagctcca gaattacgtt
    13801 gttatgagtt gtgttttgca tggcttattc ccccaccatt tcagtattcg ccgcacctgg
    13861 cggcaccatc gggtaaactt tttcttcggc atcaatgcgc acatggatca gcgccgggcc
    13921 agggcgattg atgatttcct gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca
    13981 ggtttcgagg ccgaatccgg cggcaatctg cataaagttg attttgcccg gataggtggc
    14041 ggcaaaaacg ccttgctcgt agaacagact ctgttgctga tgcaccagcc ccagcgcttc
    14101 gttgttcatc agaatgattt tgacatccag ctgattttca ctggcggtcg ccatctcctg
    14161 aatattcatc atcaggctgc cgtcgccgga gaaacacaac actttgcgat ccgggttcgc
    14221 cagcgccgcg ccaatcgccg caggcaggcc aaaacccatc gtgcccagcc caccggaggt
    14281 cagccactgg cgtgggcgat tgagcggata agcttgcgcg gtccacatct gatgctgacc
    14341 cacatcggtg gtgataatcg cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc
    14401 gtaatggctt aatggatcgc acgctttcgg gattggacac gggaattcgc gctgcaaatc
    14461 cgctaccaac tggtgccact ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc
    14521 cagcacgtca tcaacatccg cctgaatcgc cacatgcggc tgcttgattt tacccagctc
    14581 tgcgcgatcg atatcgacat gaataatttt ggcattcgga cagaactgct cggttttgcc
    14641 aatcgcccgg tcatcaaaac gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat
    14701 atagttggta ctgcgcacgc cgtgcatccc cagcataccc agcgacaacg gatgcgcttt
    14761 tggcagcatg cccagcgcca ttaaagtcat ggtggtaggc agttgcgctt tctccgccag
    14821 ttcacgcacc cgcgcaggcg cattgatcac accaccaccc agataaagca ccgggcgttt
    14881 ggcagcgtta atcattgtag ctgcgtcacg aatgctttct tcgctaaagg cgggtgcagc
    14941 ggctttttct gccaccgcgg gctgagcttc aatctcaaaa accgccgttt gtacatcctt
    15001 aggaatgtct atccacaccg ggccagggcg gcctgattgc gcaatgcgga aggcatcgct
    15061 catgacctgt gggagctctt cgatatgtct gaccagatag ttgtgtttgg tgatggggat
    15121 agagatgccg taggtgtcca cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac
    15181 ctgaccagtg atgcaaatca gcgggatgga gtccagccgc gcatcagcaa tggcggtcac
    15241 caggttagtc gcacccggcc cgctacaggc catacagacc gccggtttac catcggtgcg
    15301 cgccattccc tgagcgataa accccgcgcc ctgttcatgg cgagccagaa tatggcggat
    15361 ttgcgtgctt tggcttaagg cgtcgtaaac aggcaggata gaaccgcccg gaatgcccgt
    15421 cacaatctta atgccctgct gttccaggaa atgaacgata aattctgcgc cggtaaagcg
    15481 cttacgcgtc gatgttgtgc ccgaacttgc catgctccag tccttttctt ctgggccgac
    15541 tttccgggag gtgcttaaac gaaaaacccc gcccggtttg cgccggcggg gttttggaat
    15601 cgtgtgttgt tccagtccct acggcgcatt gccgacgacc accaccacac gcacgacgac
    15661 cactgcggcg gatggcgcag ttggtagtag ttttgcgttg aacatggaag tagtcattgg
    15721 ggaccttgtt ggttttgtgt ttaacaatat ttatacaagc acagctttac aggggagaca
    15781 atggaaaatt tttcagcaag ggaaaattga ggggttgatc acgttttgta ctgaattgca
    15841 gataacaaaa aaccccgccg gagagag
//
LOCUS       DADLSB010000091        15825 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.091, whole genome shotgun sequence.
ACCESSION   DADLSB010000091 DADLSB010000000
VERSION     DADLSB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.091"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..872)
                     /gene="adiC"
                     /locus_tag="JN331_002680"
     CDS             complement(<1..872)
                     /gene="adiC"
                     /locus_tag="JN331_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY7260796.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLG"
     gene            complement(1009..1770)
                     /locus_tag="JN331_002681"
     CDS             complement(1009..1770)
                     /locus_tag="JN331_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7260797.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(2095..4362)
                     /gene="adiA"
                     /locus_tag="JN331_002682"
     CDS             complement(2095..4362)
                     /gene="adiA"
                     /locus_tag="JN331_002682"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY7260798.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDDAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(4561..5469)
                     /gene="melR"
                     /locus_tag="JN331_002683"
     CDS             complement(4561..5469)
                     /gene="melR"
                     /locus_tag="JN331_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY7260799.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            5752..7107
                     /gene="melA"
                     /locus_tag="JN331_002684"
     CDS             5752..7107
                     /gene="melA"
                     /locus_tag="JN331_002684"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY7260800.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7222..8631
                     /gene="melB"
                     /locus_tag="JN331_002685"
     CDS             7222..8631
                     /gene="melB"
                     /locus_tag="JN331_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY7260801.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKLWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(8770..9399)
                     /locus_tag="JN331_002686"
     CDS             complement(8770..9399)
                     /locus_tag="JN331_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY7260802.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(9522..11168)
                     /locus_tag="JN331_002687"
     CDS             complement(9522..11168)
                     /locus_tag="JN331_002687"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY7260803.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(11246..12586)
                     /locus_tag="JN331_002688"
     CDS             complement(11246..12586)
                     /locus_tag="JN331_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7260804.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(13157..13876)
                     /gene="dcuR"
                     /locus_tag="JN331_002689"
     CDS             complement(13157..13876)
                     /gene="dcuR"
                     /locus_tag="JN331_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY7260805.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(13873..15504)
                     /gene="dcuS"
                     /locus_tag="JN331_002690"
     CDS             complement(13873..15504)
                     /gene="dcuS"
                     /locus_tag="JN331_002690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY7260806.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            15685..>15825
                     /gene="yjdI"
                     /locus_tag="JN331_002691"
     CDS             15685..>15825
                     /gene="yjdI"
                     /locus_tag="JN331_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY7260807.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
BASE COUNT         3862 a         3813 c         4057 g         4093 t
ORIGIN      
        1 cccagtgaac ctaagcaacc cgcagctgcg cagaaggaaa caatggcccc agcggtgtca
       61 cccaacgcca tccgtgcggc atcaccgaat ggcgaggcag aaacgcgcag tgcggcatta
      121 gggatcatcc ccataatcgc ggtggtggag agcacatagc aaacggcggc aatcaatacc
      181 ccaccaatgg tggcgatagg gacattgcgt ttcgggtttt tcaccacacc tgcggcaacg
      241 gaggcacttt ccacaccgat gaacgaccac agcgtaacgt taagggtact ttgaatcgca
      301 ccgaaggtgc ccaggccgct gacgttccat gccgccatat aggtttcacc acggaaccag
      361 aaccagccaa atacggcaat cccgacgatg gggatcagcg ccagcacagt ggcaacggcc
      421 tgcacgcggg tgatcatttt cggaccgaca atgttcagca ggacgaagat ccacagcacc
      481 acgacgcagg tgatggttaa taccaatgga tctttcagaa tcgggaagaa gtaacttaaa
      541 tatcctacgc caatgaccac catggcgata ttgccgatcc agcaggccag ccagtagagg
      601 acgttggttt gataaccgag aaacgggcca aagcagcggc gggcgtaagc gtaagaacca
      661 ccaggacttg ggtcgaggaa cgacattttg gcgtatacca tcgagagccc cagcgcaccg
      721 ataatcgtca ccaaccatcc ataaatggca atcccgccag tggaggccag gtttgcaggt
      781 aacagaaaaa cacctgaccc cataatattc cccgacacca tcagggtgac ggggattaag
      841 cccactttgt gagcatcagc atccgaagac ataattaaac tcctgcgaag gcgagcttcg
      901 tgacaataaa ttacgtcata tcatacgcct gcattcgata gttgaattat tatcgaccgg
      961 gttatcgctg atgccgttat taatatagct cttcgttaag gttaaaaatc aggcgacagt
     1021 gcgttctctg tgctgactaa cataatgcag cggcgtcata ccgtagaagt ctttaaagac
     1081 agaaataaag tacgacgtac tgttgtagcc gcaggactgt gatacctgag agatattttt
     1141 accgtccatc attaattcat ttacggcata acgcatgcgg caggtggtga ttatttggct
     1201 ataactggtg ttttcgcttt tcaacttttt ctttaacaga cttgggctaa gacataaaca
     1261 actggctacc atactaagat tccagtcttt gtgaatatcg ctttcaataa tttgataaac
     1321 gctgtcactt acacaattac gtaacatata catcattaat gaaaggaatt ttttactgtc
     1381 gagaaaacgg gatagcaccg tgaataataa tgcgcgtgtt cgttcctttt ctgactcagt
     1441 ttctgcgggc atcatgctgt gttgcgcggc aacgcgaaag acgtctggcg tcaggcatgg
     1501 cagggtgagt atgggcgtag cgctacgttg ccatactggt atttgcgaga gatctttatt
     1561 cagaaaccgg aggtaatctt tgatgatgtc gtgactaata tgagcaacca aagtgttgtt
     1621 aagagaggag acgtcgataa tattattttc acaatttaat aacgccatat ggttagcttt
     1681 aaggctaata ggttctttcc cattcaccct tatccagaca tctttttcag tcaataaaac
     1741 aatacaaggt tggtcgctgc aaatcctcat atatgtactc ctgagtgcga ataaaagacc
     1801 agttctcgct aaagcaaagc gataccggga aatggttata taattgtatg atgaatataa
     1861 acatgcataa aaataacgag ttttactaat attacaaatt tttatagtaa tttctcatgt
     1921 acatattttt ttacaggata aataacaggc aaaaaaaagc gcggggtggg gccgcgcaaa
     1981 aagtatgaca tttttgtttg cagcaatttt tatgaaaacg ttgccatact caaaaggcct
     2041 aaggccaggc ggcgtaatgt tatttaaaca attacgcctt cagcggaata gtggttacgc
     2101 tttcacgcac ataacgtggt aaataccgtc aataatttca gtcccttcag tttcgtgttc
     2161 aaatccaggg aaatggtggt cccaggattg cagcgagcgt aaataactta cttgcggact
     2221 gtttttatcg ccgaagtttt caccagacag cagcatcggg attcctggcg gatacgggat
     2281 aactgagttt gccgcgatgc gtcctggcag attttcaatg gataccagtt cgacattgtt
     2341 gtcgacaatc gcgttgtacg cttcacgcgg ggtgatttcc gccaccggca ggccggaata
     2401 ggcttcgttc aaccgtgcgc cagggttgtt ttctttcagc caggcaaaca tggtgtcacc
     2461 cagatcgtga atccccatgt tcgcgtaagt gtcaggatat tgttcaacaa gttccggcat
     2521 cacctgcgcc agcggtgtgt tggcgtcata gtggcgtttg aaggagcaaa gggtgttaac
     2581 cagagtcccc catttcccac gggttacgcc catagagaac aggaacataa tttggaagtc
     2641 agtggtgcgg gtaggcacaa tgccgtggcg accaagccag gcagtgacca gcgccgccgg
     2701 aacaccggtt tcttccagtt caccatcttc acccattccc ggagcaagga tgctgacttt
     2761 aattgggtcg agcatactcc agttatccgg aatatctttg aagccgtgcc agctttcgcc
     2821 cggatgcatt acccagcagt cctgaacggt ggtcagcagt ttggttggtg cgtcagcaaa
     2881 gtcataggtt ttgccggttt gtgggtcggt gacgacttct ttgttccacg gtttgaagaa
     2941 ccagctaccg tcagcggtga actctttata tagccgcgcc atcgcctgac ggaaatcaac
     3001 cgcgtcgtca atcacttcct gtgtcagtga caggccgctg ttgccgtcca tcatcgacac
     3061 cgccacgtcg ttggatgcgc agatggcata cagcggggag gtggtggcat gcatcatgta
     3121 ggcctggttg aagcgggaga agttaatcgc cccacgacct tcacgtacat gaatataaga
     3181 agcctgtgac agcgcattca gcagtttgtg gatggagtgg gtggcgaaaa cggtaggacc
     3241 gttgtgatcg ccaggttcgc cgcgcatggc atagtgatcg gcatagatcg ggttgaaacg
     3301 tgcatagccg taccaggctt cgtcaaagtg cagacgatcg gaggtttttt ccagcagatc
     3361 ctgcgcttct ttagcgttat aacacacgcc gtcataggtg cagttggtta ccacgcagta
     3421 agacggtttt tgcccggctt tgtctttggt cagcgggctt tcactgattt tcttttgcaa
     3481 ggtttcaggt tgcatttcct gcggatagat tggcccgata atgccgtagc ggttgcggct
     3541 tggcaccata tagaccggtt tcgcgcctgt cagcatcaaa ccttgttcga tggatttatg
     3601 gcagttacgg tcaacgacca cgacatcgtt atcggtcatg caagcctgca tgatggtgcg
     3661 gttagagccg gaagtaccga cgactaccga ccaggagcga tcggcaccaa atacgcgtgc
     3721 ggcatatttt tcgctttcgc caaatgcgcc agtatggtca agcaaagaac cgagggaagt
     3781 tcgttcgatg cccatgtcgg tgcggaacag attttcacca tagtagtcat ggtagaaacg
     3841 tccggcgggt gttttggtaa aaccaacgcc gccctggtgg cctggcgctg cccaggaata
     3901 ttcatggatg tcactatatt tcatcagcgc gctgaacagt ggcggcaaca gctgctggcg
     3961 gtagcgggtc atcgcggcaa cggcgcgtcc ggcgataaag tcggcggtat cttccagaat
     4021 ccaggcgaat tcatcgacaa gctccagcag atcccggtcc attgcggcga gggctttttc
     4081 ccgatcgccc aacaggaaga ccggcacgtt ttgttggcgc tcatgaagct taccgatcaa
     4141 ttgtctgacg ttttgatgtt cgtccggatg ttccatttga tagctgaaca tcaggcagtc
     4201 aatggcttcg tttgaagaga gaatggcaaa accatcatca aaggaggtgg atttaatcac
     4261 ggtaacattt tgctggctta aagcatctgc cagacgctca acggcgttac cgacccaggt
     4321 gtcttgatgg agaaactcgc tttcaacaat taatactttc atcattgctt acccggttat
     4381 gaaggaatct tcgttgcggg caagtatctt ccggcctttt cgggctgtaa agcgcgtgaa
     4441 aaacaaacat ttttgaaata tttagtttta taaacaatac gttatgtgat tattttaaat
     4501 tattttcact gcttattatc gacggctaaa ctattttttt ggctgatgct gatatcgtct
     4561 ttagccggga aacgtctggc ggcgctgttg gctaagtttg cggtattgtt gcggcgacat
     4621 gccgacatat ttgccgaacg tgctgtaaaa acgactactt gaacgaaagc ctgccgtcag
     4681 ggcaatatcg agaatacttt tatcggtatc gctcagtaac gcgcgaacgt ggttgatgcg
     4741 catcgcggta atgtactgtt tcatcgtcaa ttgcatgacc cgctggaata tccccattgc
     4801 atagttggcg ttaagtttga cgtgctcagc cacatcgttg atggtcagcg cctgatcata
     4861 gttttcggca ataaagccca gcatctggct aacataaaat tgcgcatggc gcgagacgct
     4921 gtttttgtgt gtgcgcgagg ttttattgac cagaatcggt tcccagctag agaggctaaa
     4981 tcgcttgagc atcaggccaa tttcatcaat ggcgagctgg cgaatttgct cattcggact
     5041 gtttaattcc tgctgccagc ggcgtacttc aaacgggcta agttgctgtg tggcaagtga
     5101 tttgatcacc atgccgtgag tgacgtggtt aatcaggtct ttatccagcg gccaggagag
     5161 aaacagatgc atcggcagat taaaaatcgc catgctctga caggttccgg tatctgttag
     5221 ttggtgcggt gtacaggccc agaacagcgt gatatgaccc tgattgatat tcactttttc
     5281 attgttgatc aggtattcca catcgccatc gaaaggcaca ttcacttcga cctgaccatg
     5341 ccagtggctg gtgggcatga tatgcggtgc gcgaaactca atctccatcc gctggtattc
     5401 cgaatacagc gacagcgggc tgcgggtctg tttttcgtcg ctgctgcaca taaacgtatc
     5461 tgtattcatg gatggctctc tttcctagaa tatcagaatt atggcaggag tgagggagga
     5521 tgactgcgag tgggatcacg gttttcaccc tcttcccaga ggggcgaggg gactgtccga
     5581 gcatcatgag gccgaaaact ctacttttca ggtaatttat tcccataaac tcagatttac
     5641 tgctgcttca cgcaggatct gagtttatgg gaatgctcaa cctggaagcc ggaggttttc
     5701 tgcagattcg cctgccatga tgaagttatt caagcaagcc aggagatctg catgatgtct
     5761 gcacccaaaa ttacatttat cggcgctggt tcgacgattt tcgttaaaaa tattcttggt
     5821 gatgtgttcc atcgcgaggc gctgaaaacg gcgcatattg ccctgatgga cattgacccc
     5881 acccgcctgg aagagtcgca tattgtggtg cgtaagctga tggattcagc aggggccagc
     5941 ggcaaaatca cctgccacac ccaacagaaa gaagccttag aggatgccga ttttgtcgtg
     6001 gtggcatttc agattggcgg ttatgaacct tgcacggtga ctgatttcga ggtctgtaag
     6061 cggcatggtc tggaacaaac cattgccgat acgttggggc cgggcggtat tatgcgcgcg
     6121 ctacgtacca ttccgcatct gtggcaaatt tgcgagaaca tgacggaagt ctgccccgat
     6181 gccaccatgc tcaactatgt taacccaatg gcgatgaata cctgggcgat gtatgcccgc
     6241 tatccgtata tcaaacaggt cgggctgtgc cattcggtgc agggaacggc ggaagagctg
     6301 gcgcgtgacc tcaatatcga cccggctacg ctgcgttacc gttgcgcagg tatcaaccat
     6361 atggcgtttt acctggagct ggagcgcaaa accgccgacg gcagttacgt gaatctctac
     6421 ccggaactgc tggcggctta tgaagcaggg caggcaccga agccaaatat tcatggtaat
     6481 actcgctgcc agaatattgt gcgctacgaa atgttcaaaa agctgggcta cttcgtcacg
     6541 gaatcgtcag aacattttgc cgaatatacg ccgtggttta ttaagctagg tcgtgaggat
     6601 ttgattgagc gttataaagt accgctggat gagtacccga aacgctgcgt cgagcagctg
     6661 gcgaactggc ataaagagct ggaggagtat aaaaacgcct cccggattga tattaaaccg
     6721 tcacgggaat atgccagcac aatcatgaac gctatctgga ctggcgagcc gagtgtgatt
     6781 tacggcaacg tccgtaacga tggtttgatt gataacctgc cacaaggatg ttgcgtggaa
     6841 gtagcctgtc tggttgatgc taatggcatt cagccgacca aagtcggtac gctaccttcg
     6901 catctggccg ccctgatgca aaccaacatc aacgtacaga cgctgctgac cgaagctatt
     6961 cttacggaaa atcgcgaccg tgtttaccac gccgcgatga tggacccgca tactgccgcc
     7021 gtgctgggca ttgacgaaat atatgctctt gttgacgacc tgattgccgc ccacggcgac
     7081 tggctgccag gctggttgca ccgttaaaac gcgactaaac gctactgcgc cgggggattt
     7141 attccggcgc acacctctga cgataccaat aacagaaggc gggcgttggt aacagcgacc
     7201 cgatacccta tgagcatttc aatgactaca aaactcagtt atggatttgg agcgttcggg
     7261 aaggattttg cgatcggcat tgtgtatatg tacctcatgt attactacac cgatgtcgtc
     7321 gggctgtctg tgggtttggt cggtactttg tttctggtgg cgaggatctg ggatgctatt
     7381 aacgatccga ttatgggatg gattgtaaat gctacgcgat cgcgatgggg taagttcaaa
     7441 ctctggatcc tgatcggtac gttggcaaac tctgtaatct tatttctcct ctttagtgcg
     7501 catctgtttg aaggtactac tcagattgtc tttgtttgcg tgacctacat cctctggggc
     7561 atgacttaca ccattatgga tattcccttc tggtcgctgg ttccaaccat cacgctcgat
     7621 aaacgtgaac gcgaacaact ggttccttat ccgcgttttt ttgccagtct ggcaggcttt
     7681 gttacggcag gtgtgacgct accatttgtt aattatgtcg gcggtggcga tcggggattt
     7741 ggctttcaga tgttcactct ggtattgatc gcctttttta ttgtttcaac catcatcact
     7801 ctgcgcaatg tgcatgaagt cttttcgtca gacaatcaat cgtctgctga aggaagccat
     7861 ctgacactta aagccatcgt tgcgcttatt tataaaaacg atcagctttc atgcctcttg
     7921 ggtatggctc ttgcttataa tgtagccagc aacattatta ccggatttgc tatctattat
     7981 ttctcatatg ttatcggtga tgcggatttg ttcccctatt atctgtcgta tgcgggagct
     8041 gctaacctgg tgacgttagt attcttccca cgcttagtta aatcattatc ccgacgcatt
     8101 ttatgggccg gagcatctat tcttccggtg ttaagctgtg gtgttctcct gttaatggca
     8161 ttaatgagct atcacaacgt cgtcctcatt gtgattgcgg gtattttgct gaatgtggga
     8221 acggcgcttt tctgggtatt acaggtcatc atggtggcag ataccgttga ttacggtgaa
     8281 tataaactgc acgtacgctg tgaaagcatc gcttactccg tgcagactat ggtggtgaag
     8341 ggcggttcag cctttgcggc ttttttcatt gcggttgtgt tagggatgat tggctatgta
     8401 ccgaatgttg aacagtctac gcaagcccta ttaggtatgc agtttattat gattgctcta
     8461 ccgactctgt ttttcatggt aacgctgatt ctctacttcc gtttctatcg cctcaatggc
     8521 gacacgctgc gcaggatcca gattcatctg ctggataaat atcgcaaagt accgcccgag
     8581 cctgttcatg ctgatattcc ggtcggtgca gtgagtgatg tgaaagcctg acgtggacaa
     8641 tagctaacga aaatggcctg atgcgatatg tttatcaggc cacgtcttaa tgtaatgctt
     8701 tggatttgca aattttgtag accggataag gaattcacgc cgcatccggc atcaacaaag
     8761 tgcacgttgt tatccggtta tcaagccata gcgccgtagc tggcggcaat gaaagcgagc
     8821 gagcaatatc actgtcgtta atgcgccaag cagcgcgcag aacatatcgg attgcgtgtc
     8881 ccactggtcg ccctgggtac cgagaaaatc atccgctccc tggcccatcg ccagtgccgc
     8941 ccaccactcg attaattcat acatcgcgct tatcgccagc gccacgcaac agaccagaaa
     9001 cgccaccatt ttacgtccgc gcacgtacat cccgcgaacg agaatttctc gtgccaccag
     9061 tgcaggcacc agcccctgga aaaagtgccc cagcttgtca tacggattac gactcaaccc
     9121 cagccattcc tgcacctcaa aaccaacggg gactttcgcg taggtgtact gcccgcctac
     9181 catcaggatg atggcgtgaa agaagatgag cgtatagagc agcagcgtta acgggtaacg
     9241 tctggcggtg gcaagtagta gcggcacgac gataatcacc ggtgtcactt ccagcagcca
     9301 ggtgagtttg tcatgggccg aaatgccggt ataaatcagg attaacgtta gcgccagtgc
     9361 gccggtgtta agaattaacg gcttgagtgt gcgggtcatg gtgagttcgc aagtcaggga
     9421 aaatactgac tattcaccag caagcatgaa attacaatcg caaaagaggt ggcccagggg
     9481 gatcacctgg cagcatgctg ccaggcgctg ggccgaagag gttacttagt gcagttcgcg
     9541 cactgtttgt tgacgatttg ctggaagaag tcgttacctt tgtcatcgac caggataaac
     9601 gccgggaaat cttctacttc gattttccag atagcttcca tacccagctc cggataagcg
     9661 acgcattcca gatgcttgat gctctgctgc gccagtaccg ccgccggacc gccgatgcta
     9721 ccgaggtaga agccgccgtg tttatgacac gcgtcggtaa cctgctggct gcggttgcct
     9781 ttcgccagca tgatcatgct gccgccgtgg gattgcagca gatccacata ggagtccata
     9841 cgacctgcgg tggttgggcc aagtgaacct gatggataac cggcaggggt tttcgccgga
     9901 cccgcgtagt agatcgggtg atctttgatg tactgcggaa gttctttacc ggcgtcaatc
     9961 agctctttca gcttggcgtg tgcaatatct cgacccacaa taatggtgcc agtgagcgac
    10021 aaacgagtgg aaaccggata ttgcgaaagc tgggcgagga tctctttcat cgggcggtta
    10081 aggtcgacct tcaccgcttc gccttcaccg gcctgacgca gttcttgtgg aatgtactga
    10141 cctagattgt gttccagttt ttcgatccag ataccttcgc ggttgatttt cgctttaatg
    10201 ttacggtcag cggagcagga gacgcccatg cctaccgggc aggatgcgcc gtgacgtggc
    10261 agacggataa cgcgaatgtc gtgcgcgaag tatttaccgc caaactgcgc gccaagaccg
    10321 agtttctggg cctcttccag cagttcctgt tccagctgga catcgcggaa cgcctgacca
    10381 tgttcgttcc cttccgtcgg cagttcatcg taatagtgag cgcttgctaa tttgacggtt
    10441 ttcaggttgg tttccgcaga cgtaccgcca atcacaaacg cgatatggta cggcgggcag
    10501 gctgcagtac cgagggtacg cattttctcg acgaggaagt ttttcagttt gccgggagtc
    10561 agcagggctt tggtttcctg gtagagatac gttttgttgg cagagccgcc gcctttcgca
    10621 acgcaaagga atttgtactc atcgccatct accgcgtaca ggtcgatttg cgcaggcagg
    10681 ttagtgccgg tgttgacctc tttgtacatg tccagcgccg cgttttgcga gtagcgcagg
    10741 ttatcttcga tataggtgtt atagacgcct ttcgacagcg cttcttcatc accgccaccg
    10801 gtccacacgc gctggccttt tttaccgacg atgatcgcgg tgccggtatc ctggcaggtc
    10861 ggcagcacgc ctttggcggc gatttcggag tttcttaaga attgcagtgc cacgtacttg
    10921 tcgttttcgc tggcttctgg atcgtgaaga atagccgcaa cctgtttctg gtgtgccgga
    10981 cggagcatga aagaagcgtc gtgaaaggct tgctgcgcca gcagggtcag ggcttctggt
    11041 tccactttca ggatggtttc gccgtcgaag tcggcaacgc taacgtaatc ggaagtgagt
    11101 agatagtatt cggtattgtc tttccccatc gggaaaggtg cttggtagat aaagggtttg
    11161 tttgacatag cttccagcct gtaactctgt atttgttatt cgaaaatggc gtgccgcgta
    11221 gtcacgggcg gcacgcaaag tgcatttata agaacccgta catcgcggcg aagatccagc
    11281 cgaagacgca cgatacgctc acaccaatca accccggcag aataaaactg tggttgatga
    11341 cgaagcgacc gatgtgggtg gtgccggaac ggtcaaactg aatcgctgcc agatcgctcg
    11401 gataagtcgg caggatgtaa taaccgtagc aagccggtgc tgaagccacg atgtatgccg
    11461 gatcaacgcc gatcgccagc gcgaccggaa caatcgccgc cagcgccgca gcctgagagt
    11521 ttacaaactt ggaaaccagc agcagaacaa tggcatacgc ccacggatac tctttcacca
    11581 tttcacccag tacgccctga atttcagaca tatgcgcacc gaacatggtt tctgccatcc
    11641 atgcgatacc gtacaccgcc acgatggcga tcataccgga acggaagact tcgttttttg
    11701 agatagacgc gggattggtt ttggtcagga taataatcag cgccccggtc agcagcataa
    11761 acatctgaat aaccagtacc atcgacagcg gtttgccgcc gaaggatgga cgcaggtccg
    11821 aatcagcacc aagaagggcg actacagcga ttgccccgag gaaaatccac attgccagcc
    11881 agttgctttt cggcagtttt ttatccagca gcgtcgcggt atcaccgtaa acatactcac
    11941 ggttttccgg tacggagatg aatttctgga actcttcgtc tttatccaga tctttaccgc
    12001 ggaaccagct gaagataccg atcgccagga taccgattaa cgtcgatgga atggtgattg
    12061 ccagcagatc gaggaactca agatggcgac catcaaaggt gacattaccc agcatcgcaa
    12121 ccagagacac gaccgcaacc gacaccggac tggcgataat ccccatctgt gcaccgatag
    12181 aacttgccgc catcggacgt tccggacgga tgttgttctt aatggcgacg tcgtagatga
    12241 tcggcagaat ggtgtaaacc acatgacccg taccgcaaag aatggtcagt gtacaggtca
    12301 caaacggcgc gacaattgag acatatttcg ggttgcggcg cagcagcttc tcggcaattt
    12361 gcagcatgac atcaagaccg cccgaagctt gcaaggtcgc cgatgccgcc accaccgcaa
    12421 tgataaccag catgacatca actggtggtt tacctggctg aaggtggaag acgaagacca
    12481 gaatgaccag accgataccg cctaataaac ccagcgcgat accacccttt ctggcaccat
    12541 aaaacagaca tatcagtatt atgataagtt ggatagtaaa taacatgtgt gaaccctcgc
    12601 gataatccta tttaaatttt tgctgaatag atcacagtca cgttctgttt tgtatgaact
    12661 gttttcagag cctgactaaa tatccgtttg gtcgttaccg gctttagcaa atacctcaca
    12721 gtgaatattg gctgattaaa gcagcggcaa atttatgcac tgtgataagc ggctttttca
    12781 gagagaaaaa ctctctggcg atgggtttat agtttgtact gcgcagatgt taatgttttg
    12841 tttgctgtta tcttattgat atttatcggt ctattttttg ttctcagggc agatatcatt
    12901 aggtttatag attaatctga tctacccatt tgtgggtaaa aatacacata atgcgggtga
    12961 cataatagtt aattaacttt tgttatcgtt ttgaaattaa aaacaccgtt catctgaaga
    13021 gatattaatt tttaacgatg atggagggat aattatattt gatctggcac aagttttact
    13081 gattaacgat gtaacttgtg ccaggggtat tttgcattac ggtaataatt attacttaca
    13141 gataaccagc gatccgttat tggcaatatt gtttcagcag tgagtagtgt tctgcctgaa
    13201 tacggtaacg gtaaaccgga cgccccgtga cgccataatg gatactggtg aacaagatgt
    13261 ggcagttgac cagccagatg aggtattttc ggcaggaaac acgcgagatg ttaacctcgt
    13321 tagccagctc gtcggttgaa aactcatagt cctgatgcgc gtcaatccac tggcacagcg
    13381 tgcgtaacgt ctgcggcgtt aagccttttg gcaagcgacg aggatcctgt tcgttggagc
    13441 tgctgccgtg gattagctga tcaagctcgg cctggtcata atactgatgt ttttccagcg
    13501 ccattttctt ttgccgccag ccggtgagcg cctcttcaaa gcgggaagcc tggaagggtt
    13561 tgatcaggta atccacgaca ccgtaatgca gcgaatcttt aatggttgcc gcatcggctg
    13621 cggaggagat gacaatcaca tcacttttgc aacgcgcgtt atgcaggaca ggcagtaaat
    13681 cgagcccgtt ctctttttgc atatagatat cgagcaatat caggtcgata ggcgcatcgc
    13741 tattgaagat aatctctttg gctttctcca gcgtcgaggc tgttccacag cattgaaagc
    13801 ctgggatttg tgctacgtat cggcgattca gctccgcgac cattgcgtcg tcatcgataa
    13861 ttaatacatt gatcatctgt tcgacctctc cccgtcccag ggtatctgga caaaaaattg
    13921 tgtgaaaatc cccggttccg attccacggc gatgctgccg ccgagatttt ctacctgttg
    13981 tttgacaagt gctaaaccga cgcctcgctc gcttcctttt gtcgagacac ctttgtcaaa
    14041 aatgtgatcg attttgtcgg gtgcgatccc cggtccatca tcgttaactt cgcagtgcag
    14101 ccagccgtga cggtagtgca atgttacgct aatttcgcca ccgggttccg gccctaatgc
    14161 ttccagcgcg ttttctatca gatttcccaa tgtggtaatc agcgtcgcga cctggtcctc
    14221 actgccgctg tctggcagct ggctttcact gtttaaaatc agcgtatggc ctaaatcggt
    14281 cgcgcggtta atcttgctga ttaaaaaacc agcgataacc ggagatttga tcttacccag
    14341 cagagagcca atctcttcct gatagttatt ggctgttttg agaatgtaat cttccaactg
    14401 cttataactc ttcagatgca ataatccgag aatcacatgc aatttattca taaattcgtg
    14461 ggatcgttca cgaagtgcgt cagcatagtt gaccagaccg tcgagtcgct gcatcagttt
    14521 acgaacttca gttttgtccc tgaaggttga aatggcaccg atgataacgc cattactgcg
    14581 caccggaacg gtgttgatca gtaatagccg gtctttaatc gtaatctctt cgtcgcggcg
    14641 cggcgtaccg tcgcgtaaca cttccgagac atctaccacc tgtgaccatg agtggctgag
    14701 cgtcgacagt ttctcatcgt cctgcgactt acggtaattc agcaattctt gtgcggcatc
    14761 gttgatcagc gtgacctcgc cgcgatcgtc cacggcaacg acgccttctt tgatagactg
    14821 caacatggcc tggcgttgct caaacagcgt ggagatttcg tagggttcca ggccgaaaag
    14881 gatttttttc agtaccttaa ccagaatgca ggtgccaatc agtccgacca gcatgccaaa
    14941 taataccgac cagataatgc tccagcgact gtcattgatc tgttgggtca cgcggcttaa
    15001 ctcaaggccg atcgccacca cgccaatttg tttatgattt tcatcgtaga tgggggtaaa
    15061 tacgcgtaaa gcctgcgcca gaaaaccgcg attgatagcg acattttctt cgccattcag
    15121 cgcattaagg atgtcatcac ctttaaatgg ctgaccaata cgctgggctt caggatgtga
    15181 gtagcgaaga ctttgcatat cggtaacgac aataaacagc agatcgttgc gtttgcgcac
    15241 ggcttccgcg atggcctgga tgccactctc ctgcggtttt ttctgcaagc cctgacggat
    15301 ttccggcgag tcggcgaggg tacgcgccac tgccagtgcc ttgttggcca gcccgtctcg
    15361 cgtcatatca ctgatttgcg agaagtaaat cagatgcacc accaatagca ccgagaacag
    15421 taccgcgctg accattaaga tcacagtggt actcaatttc atcggacgtt tgcgtaatat
    15481 gtggtagggc aatgaatgtc tcatcagctt ccttgtgtga caaatttctt aagcattatc
    15541 tctgatgagg cgggtaattc aaagggagta aaaatgattg gctatatagg ggaagagact
    15601 ctggcaacgg aaactgccag tgctgtatga agattccggg gctatgctta tagcgataat
    15661 catactgatg agagagggaa ggtcatggat caggcgctac tggacggggg ttatcgctgt
    15721 tataccggcg aaaagatcga tgtctatttc aacactgcga tatgtcagca ttctggcaat
    15781 tgcgtacgtg gcaacggcaa gttatttaat ctcaaacgaa agtcg
//
LOCUS       DADLSB010000092        15658 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.092, whole genome shotgun sequence.
ACCESSION   DADLSB010000092 DADLSB010000000
VERSION     DADLSB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.092"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..848
                     /locus_tag="JN331_002692"
     CDS             <1..848
                     /locus_tag="JN331_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7260808.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLAPDPA
                     ALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTF
                     NPAHAQQKIASADLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDA
                     QLRKADPVAWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLR
                     RYGTH"
     gene            821..2357
                     /locus_tag="JN331_002693"
                     /pseudo
     CDS             821..2357
                     /locus_tag="JN331_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2357..3010
                     /locus_tag="JN331_002694"
     CDS             2357..3010
                     /locus_tag="JN331_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7260809.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            3077..4384
                     /locus_tag="JN331_002695"
     CDS             3077..4384
                     /locus_tag="JN331_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAY7260810.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVENEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(4393..5013)
                     /locus_tag="JN331_002696"
                     /pseudo
     CDS             complement(4393..5013)
                     /locus_tag="JN331_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
     gene            5100..5507
                     /gene="nudG"
                     /locus_tag="JN331_002697"
     CDS             5100..5507
                     /gene="nudG"
                     /locus_tag="JN331_002697"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY7260811.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(5473..5745)
                     /locus_tag="JN331_002698"
     CDS             complement(5473..5745)
                     /locus_tag="JN331_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY7260812.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            5981..7324
                     /gene="gdhA"
                     /locus_tag="JN331_002699"
     CDS             5981..7324
                     /gene="gdhA"
                     /locus_tag="JN331_002699"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY7260813.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(7441..8481)
                     /locus_tag="JN331_002700"
     CDS             complement(7441..8481)
                     /locus_tag="JN331_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7260814.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSATLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(8609..10570)
                     /locus_tag="JN331_002701"
     CDS             complement(8609..10570)
                     /locus_tag="JN331_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY7260815.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(10575..11618)
                     /gene="selD"
                     /locus_tag="JN331_002702"
     CDS             complement(10575..11618)
                     /gene="selD"
                     /locus_tag="JN331_002702"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY7260816.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(11735..12286)
                     /locus_tag="JN331_002703"
     CDS             complement(11735..12286)
                     /locus_tag="JN331_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY7260817.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGGERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            12447..14303
                     /gene="sppA"
                     /locus_tag="JN331_002704"
     CDS             12447..14303
                     /gene="sppA"
                     /locus_tag="JN331_002704"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY7260818.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSPHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            14470..15486
                     /gene="ansA"
                     /locus_tag="JN331_002705"
     CDS             14470..15486
                     /gene="ansA"
                     /locus_tag="JN331_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY7260819.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNASQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            15497..>15658
                     /locus_tag="JN331_002706"
     CDS             15497..>15658
                     /locus_tag="JN331_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY7260820.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWH"
BASE COUNT         3647 a         4155 c         4052 g         3804 t
ORIGIN      
        1 taaaagaggc caatttatta caaacgggct gggcggagac tctgcccaac tggcgctatg
       61 tcgacacaca gctgccggtg cgggaagatt tttcagtgcc gacacaaggt gcggaatcgc
      121 cctggggcgg cgcacaactg acgtttatcg cccgccgcga tgttacgcca cagccaccac
      181 aaacgccgca agccttactg gagtttgcta aagccaatcc cggcacggtt acttatccgc
      241 gcccaccgga ctttaccggc acggcgtttc ttgaacagtt gctgattatg ctggcgccag
      301 atcccgccgc attaaaagaa gcgccggacg atgcgacttt cgcccgtgtc actgcgccct
      361 tgtggcaata tcttgatgca ctgcatccgt atttgtggcg cgaaggaaag gatttcccgc
      421 cttcgcccgc gcggatggat gctctgctga aagccggcac attgcgcctg tcgctgacct
      481 ttaaccccgc gcatgcgcag caaaaaatcg ccagcgccga tttgccggca agcagttaca
      541 gttttggctt tcgcgagggg atgattggca acgtgcattt cgtcaccatt ccagccaacg
      601 cgaatgccag tgctgcggcg aaggtagttg ccaatttcct gctctcaccc gatgcgcaac
      661 tgcgtaaagc agatcccgtt gcctggggcg atccttctgt tctcgatccg caaaaactgc
      721 ctgacgggca gcgcgaaata ttgcaatcaa gaatgccgca ggatctgccg ccggtactgg
      781 ctgaaccgca cgcaggatgg gtgaatgcac tggaacaaga atggttacgc cgttacggta
      841 cgcattaatc tttttgctgt gggcgatggt ggcggtgatt tatgcaccgc tgatcccggc
      901 agctctcacg ctgatatcgc ctgcgttgtc gttgacacgc tggcaggcgt tatttgccga
      961 tccacagtta ccgcaggcat tactggcaac gctggtgtcg acaaccatcg cggcggtcgg
     1021 ggcattgttg attgccctgc tggtgattgt ggcgctatgg cctgggccga aatggcagcg
     1081 tatgtgcgcc cgtctgccgt ggctgctcgc cattccccat gtggcttttg ccaccagcgc
     1141 cctgctgctc tttgctgacg gagggctgct atatgactat ttcccgtatt tcactccgcc
     1201 aatggaccga tttggcatcg gtctgggcct cacccttgca gtgaaagaaa gcgcatttct
     1261 gctgtggatc ttagcggcag tattgagcga aaaacggctg ttgcagcagg tcattgtgct
     1321 ggattcgctg ggctacagcc gctggcaatg cctgaactgg ctgctgttgc cctccgtcgc
     1381 gccttcgctg gcaatggcga tgctggcgat tgttgcctgg tcgctttcgg tcgtggatgt
     1441 ggcgattatt cttgggccag gtaatccacc gacgctggca gtaattagct ggcagtggtt
     1501 aactcagggc gacgcggatc aacaaacgaa aggcgcactc gccagtctgc tgctgatgct
     1561 gttactcgcc gcctacgttt tgctgagcta tctgctatgg cgcagctggc ggcgcactat
     1621 tccccgcgta gatggcattc gcaatgcctg ccacaccttt attaccaggc actacgctgg
     1681 cgagtttttt acccttaacc ggtgtgctgt gtgtggttct gctggcgatc ctcgcggatc
     1741 agtcgacgat caatagtgaa gcactcatca acagtctgac aatggggctg gtagcaacat
     1801 tcatcgcttt gctcctgtta ctgctgtggc tggaatgggg ggcacagcgt cgccagttgt
     1861 ggctatggtt gcccattcta ttgcccgctc tgccactggt ggcgggccag tacacgctgg
     1921 cgctatggct gaacctggat ggaagctgga cggcggtggt ctgggggcat ctgctgtggg
     1981 taatgccgtg gatgctgttt atcctgcaac ccgcctggca gcgcattgat tcacggttaa
     2041 ttttgattgc gcaaacactg ggctggtcgc gggccaaaat cttcttttac gtgaaatgcc
     2101 cactcatgtt gcgccctgtg ctgattgcct tcgcggtggg attttcagtc agtattgcgc
     2161 agtatatgcc gacgctgtgg ctgggcgcgg ggcgttttcc gacgctcacc actgaggcgg
     2221 tggcattaag cagcggcggc agcaacggta ttctcgccgc ccaggcttta tggcaactgt
     2281 tgttaccgct tattattttt gccctgaccg cgttagtcgc aaaatgggta ggttatgtca
     2341 gacaaggaat ccgctaatgc tctgcgtgaa aaatgtttcg ctacgtttac cagaaagccg
     2401 cttgctgaca aacgttaact ttacggttga taaaggtgac atcgtcacgt taatggggcc
     2461 gtctggctgt ggaaaatcca ctctgttttc atggatgatt ggtgcactgg ccggacagtt
     2521 ttcttgtaca ggtgagctat ggctcaatga gcaacggatt gacatgctac ccaccgcaca
     2581 gcgtcagatt ggcattcttt ttcaggatgc actgttattt gaccagttca gcgtcgggca
     2641 aaatttactg ctggcgctac cggcgacact taaggggaat gcccgacgta atgccgtgaa
     2701 tgatgcactt gagcgttcag gccttgacgg agctttccat caggatcctg ccactttgtc
     2761 tggcggtcag cgagcgcgcg ttgctctact acgcgccctt ctcgcccagc caaaagcgtt
     2821 actcctggat gagccattca gccgtcttga tgtggctctg cgcgataatt ttcgccagtg
     2881 ggtgttcagc gaagttcgcg ccctggcgat ccccgtcgtt caggtaacgc acgatctcca
     2941 ggatgttcct gctgatagtt ctgttctgga tatggcgcag tggtcagaaa attacaacaa
     3001 actgcgataa cgcaaagttt ttctcaatgc gtcagttcag aatggcgcac tcaaaactac
     3061 aatgtcggga ttttcgatga aacgtgtttc tcaaatgacc gcgctggcaa tggctttagg
     3121 gctggcttgc gcttcttcgt gggccgctga actggcgaag cctcttacac ttgaccaact
     3181 tcagcaacaa aatggcaaag cgatagatac tcgccccagc gcgttttata acggctggcc
     3241 acaaacctta aatggccctt ctggtcatga acctgccgcc ttaaacctct ctgctagctg
     3301 gctcgacaaa atgagcaccg aacagctcaa cgcgtggatc aagcaacata acctgaaagc
     3361 cgatgccccg gtggcgctgt acggtaatgg caaagatgtc gacgccgtca aaacgcgact
     3421 gcaaaaagca ggttttacgc atatctccat tctgagtgac gcgctaagcg aaccttcccg
     3481 tctacaaaaa ctgccgcact ttgagcagct ggtttatccg caatggctgc atgacctgca
     3541 acaaggtaaa gaggttacgg cgaaacctgt cggtgactgg aaagtcattg aagcggcctg
     3601 gggcgctcct aagctttacc ttatcagcca tattcctggc gctgactaca tcgataccaa
     3661 cgaagtggaa aacgaaccgc tgtggaacaa agtttctgat gaacaactaa aagcgatgct
     3721 ggcaaaacac ggcattcgcc atgacaccac ggtcattctg tacgggcgtg acgtatacgc
     3781 tggagcgcgt gtggcgcaaa ttatgctcta cgcaggcgtg aaagatgtgc gtctgctgga
     3841 tggcggctgg caaacctggt ccgacgcggg actgcctgtt gagcgaggaa cgccaccgaa
     3901 agtgaaagcg gaaccggatt tcggcgtgaa gatcccggca caaccgcagt tgatgctcga
     3961 tatggaacag gcccgtggac tgctgcatcg ccaggatgca tcgctggtga gcattcgttc
     4021 gtggccagaa tttatcggta cgaccagcgg ttacagctat attaaaccaa aaggtgaaat
     4081 agccggagca cgttggggac acgctggtag cgactcgacg catatggaag atttccataa
     4141 cccggatggc accatgcgta gcgccgatga tattaccgct atgtggaaag catggaatat
     4201 caaaccagat cagcaagttt cattctactg cggcaccggc tggcgcgcgt ccgaaacctt
     4261 tatgtacgca cgcgccatgg gttggaagaa tgtttccgtg tatgacggcg gctggtacga
     4321 atggagcagc gatccaaaaa atccggttgc aaccggtgaa cgcggcccgg gcagtagcaa
     4381 ataacattgc aattactgac gctggagcga cttcagcgtc agatacccac tccacacccg
     4441 cgtaaaggtc gtcatccagc acaacgcacc gaagattcac gcaaaccacg caaaccatgc
     4501 agggaataaa catcccagca caaacagtaa gatcgtttct gtgccttcgg ttaatccccc
     4561 cagatagtaa aacgacttat gtgcataacc ggggttatca atctgatgtt ttgctgccag
     4621 tgcagcaaag gcgagaaaac tgctgcccgt gccgataaac gcaaacaaca accagccgcc
     4681 tgccagcgca ttttgctccg gtgcagcaag aataaagcca aacggcacca gcgcgtaaaa
     4741 gagaaaatcg agagaaatat caagaaagcc gcccgcgtcg gtaagccctc tgcgccgcgc
     4801 cagcgcgccg tccagaccat caagcagcct gttcaacaaa atgacgacta gcgccgccag
     4861 ataccagccc agcgccagaa acggcagagc cagcacgccg atggcaaatc ccactaatgt
     4921 taaaccatct ggcgtaatgc ccggtttatc aagaacccgc acgcactgat gcaacaacgg
     4981 tttaatccgg ggatgaagat ggcggtctag cacaggcact ccttaaatat aaagcctttc
     5041 tgattgagca acagtgcgga tattatggca tttttcgctt atctgcccgt gtgtaattta
     5101 tgaaaatgat tgaagttgtt gccgccatca ttgaacgtga tggcaaaatt ttactcgcgc
     5161 aacgccccgc ccagagcgat caggcgggat tatgggagtt tgccggtggt aaagtcgagc
     5221 tggatgaaag ccagcagcag gcgctggtgc gtgagttaaa cgaagaattg ggcatcgaag
     5281 caactgtggg tgagtatgtt gccagccatc agcgagaagt ttcgggccgg attatccatc
     5341 ttcatgcctg gcacgtaccc gacttccacg ggacgttaca ggcacatgaa catcaggcgc
     5401 tggtctggtg ctcacctgaa gaggcgctgc aatatccgct ggcccctgct gacattccat
     5461 tattagaggc gtttatggct ttacgcgccg ccagagcagc ggattagtgc taagggtttt
     5521 gtcatcacgc tggcattgca gcagtattcc ttcggcttta attaccgccc cttcagaata
     5581 attttgatcc tgataaacgc agcactgagt acagggctgc gctgactgcc cgcctgaact
     5641 gaacacttct ggcggtacgt ttacctccac gtccggacga taatgcgggt tagccagtgc
     5701 aattaatgga aatgctaata ctacggcgaa caatgctcga ctcacaggga actccttaac
     5761 gttattgtct ctgctactga taacggtagc cgggtggcaa aactttagcg tctgagttat
     5821 cgcatttggt tatgagatta ctctcgttat taatttgctt tcctgggtca tttttttctt
     5881 gcttaccgtc acattcttga tggtatagtc gaaaactgca aaagcacatg acataaacaa
     5941 cataagcaca atcgtattaa tatataaggt ttttatatct atggatcaga catattctct
     6001 ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat caaaccgagt tcgcgcaagc
     6061 cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa caaaatccaa aatatcgcca
     6121 gatgtcatta ttggagcgtc tggttgaacc ggagcgcgtg atccagtttc gcgtggtatg
     6181 ggttgatgat cgcaaccaga tacaggtcaa ccgtgcatgg cgtgtgcagt tcagctctgc
     6241 catcggcccg tacaaaggcg gtatgcgctt ccatccgtca gttaaccttt ccattctcaa
     6301 attcctcggc tttgaacaaa ccttcaaaaa tgccctgact accctgccga tgggcggtgg
     6361 taaaggcggc agcgatttcg atccgaaagg aaaaagcgaa ggtaaagtga tgcgtttttg
     6421 ccaggcgctg atgactgaac tgtatcgcca cctgggcgcg gataccgacg ttccggcagg
     6481 tgatatcggc gttggtggtc gtgaagtcgg ctttatggcg gggatgatga aaaagctctc
     6541 caacaatacc gcctgcgtct tcaccggtaa gggcctttca tttggcggta gtcttattcg
     6601 cccggaagct accggctacg gtctggttta tttcacagaa gcaatgttaa aacgccacgg
     6661 tatgggtttt gaagggatgc gcgtttccgt ttctggctcc ggcaacgtcg cccagtacgc
     6721 tatcgaaaaa gcgatggaat ttggtgctcg tgtgatcact gcgtcagact ccagcggcac
     6781 tgtagttgat gaaagcggat tcacgaaaga gaaactggca cgtcttatcg aaatcaaagc
     6841 cagccgcgat ggtcgagtgg cagattacgc caaagaattt ggtctggtct atctcgaagg
     6901 ccaacagccg tggtctgtac cggttgatat cgccctgcct tgcgccaccc agaacgaact
     6961 ggatgttgac gccgcgcatc agcttatcgc caatggcgtt aaagccgtcg ctgaaggggc
     7021 aaatatgccg accaccatcg aagcgactga actgttccag caggcaggcg tactgtttgc
     7081 accgggtaaa gcggctaatg ctggcggtgt agcaacgtcg ggcctggaaa tggcacaaaa
     7141 cgctgcgcgc ctgggctgga aagccgagaa agttgacgca cgtttgcatc acatcatgct
     7201 ggatatccac catgcctgtg ttgagcatgg tggtgaaggt gagcaaacca actacgtgca
     7261 gggcgcgaac attgccggtt ttgtgaaggt tgccgatgcg atgctggcgc agggtgtgat
     7321 ttaagttgta aatgcctgat ggcgctacgc ttatcaggcc tacagatggg cacaattcat
     7381 tgcagttacg ctctaatgta ggccgggcaa gcgcagcgcc cccggcaaaa tttcaggcgt
     7441 ttatgagtat ttaacggatg atgctcccca gggaacattt cttatgggcc aacggcattt
     7501 cttactgtag tgctcccaaa actgcttgtc gtaacgataa cacgcttcaa gttcagcatc
     7561 cgttaacttt ctgcgatagc agcagatatg ccagtaaaga aatcccatta gactattttt
     7621 ttgataatct tcttcgcttt cgaacaactc gtgcgccttt cgagaagcaa gcattatata
     7681 atgccaggcc agttcttctt caattgtccc gttttgaaaa gctgtgcttg atatcgagat
     7741 catccatgat aattccgccg cccatattag cttcgccgag gatttaccgg agcgattatt
     7801 agcgcaatca gagatatagt ctgagggaaa aacagcaaat ttattcaaca aggcgataac
     7861 ctgctctggg gcttcctcca tgtttgcttt aaaggtattg gctccatggt cgccagaaag
     7921 aaaatgctcc attaaggcac aataactttc gctatcttcg ataccccatt gattctctaa
     7981 agactcgcgt cttttactga tgatatcgat cgagtcaaaa ggaagcacat gatattggaa
     8041 ggtatctttg ccaggttcag gctttcgcgg ccagaactct agcgttgcag accattgctt
     8101 atgatagaat cgataaggtg cgatcaattg tagcgcctgt aacttctcga tactgagcgg
     8161 ctcaatacct ttagcctgat aataatgcag ttgttctttt tttgctttaa aaccggcccg
     8221 aacaataagc cccatcataa ttaatagata aagaaaagag catcccgcgg taatcaggcc
     8281 tctttcattc aaaccgttgg atgttatcgc tgcgaataca aacattacag cgacaacaca
     8341 tgttaaataa aacccccact tacaaagcag catggcctta ttttctttaa tcacccgttc
     8401 aaaattacta ttaaatattt cccagccatt aaaagaatac ttctcgctcc caggatggtt
     8461 ttgtaataaa acttttttca tcacattccc tgttattaca tactttagag gcgataattt
     8521 tatccatgca aaaaaaatat ccaacgaatt ttcttgatcc gctggatatt tcagaatatg
     8581 actcgaatag cacgaaagat tcgctcgctt acgctatcgc cccgcttccg gcttcatctg
     8641 ctggcggact ttttttcgca ctacgtttac gcggtgcagc ctttttctta tcagcactgc
     8701 caccactgcc cggagccaca atgccgcgaa actgccgcac cggcgtacgt ttggcttgat
     8761 caataagctg atatagcgtc cccaccagcg gctgcataaa gtcctgatag cgacactgct
     8821 tttcgctgat ttgcgtcagc accgattccc agtgcgcggt catgtccggt cgcgtcgcca
     8881 tttctggcag cgaatggaat aacgcttttc cggcgtcggt ggagtggata tagcgccctt
     8941 ttttggtcag aaaaccacgc ttgaacagca gttcaataat cccggcacgg gttgcttctg
     9001 tccccagacc atcggtcgca cgaaggacct ttttcagatc tttgtcctgc acaaagcgcg
     9061 cgatcccggt catcgccgaa agcagtgttg catcggtaaa atggcgcggc ggctgggttt
     9121 gccgctctac cacttcacct ttttcacaca gcaactcatc gcctttcgcc accacaggca
     9181 atggcgtgcc gtcgttttct tcatcgcgct ctttgctgcc taacagcgtg cgccagcctg
     9241 cttcagcaag aaaacgggct ttagcgacaa atttgccttt ggcaatgtcc agttcgataa
     9301 cacacttgcg gaacaccgca tccgggcaga actgcatcag gtattgacgg gcaatcaggt
     9361 tatagacctt cgcttcgttc tccgtcaggt tgatcgcaga actccgtgcg gtcgggatga
     9421 tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg gttgcgtata tctggatcta
     9481 ccactggctg cggcaacaga tccggtgcat gaacgctgat ggcattcatc accgcgtggc
     9541 gtccggcaaa atgttcttct ggcaaatagc gacaatcgga acgcggataa gtgattagct
     9601 tgtgcgtttc gtacagcttc tggcagatat caagcacgtt ctgcgcgctc agaccgaagc
     9661 gttttgccgc ttcaatctgc aacgccgaaa gcgaaaacgg cagcggcgcg gattctgatt
     9721 cccgtttatc gttatagctg gtgacaatag ccggttgacc actaatgcgg ttaaccacat
     9781 gctccgccag tggacgatgt aacaagcgcc cttcttcatc ctggtacggt tcacacgctt
     9841 cgctcggttg ccagatagcg gtaaaccgct catcggcagg tgtcacgata tgtgctttga
     9901 cttcaaagaa gtctttcgcc acgaagtttt caatctcttc atcgcggcgc accaccagcc
     9961 cgagcacggg tgtctgcacg cgtcccacgg aaagtacgcc ctgataaccg gcattgcgac
    10021 cgagaatggt atacgcacgg gtcatattga tgccgtacag ccagtcggca cgcgctcgcg
    10081 ccagcgcaga aacgcacaat ggcacaaact cactgttgga acgaagacgg tcgatcgccc
    10141 gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg ctgcacctgc tggcgctttt
    10201 ccggtgccag ttgcagatag tccagcactt catccaccag caattgccct tcacgatccg
    10261 ggtccccggc gtgaacgatt tcgctggctt catgcaggaa gcgtttgatg acgttaagtt
    10321 gtttggtcac ggagggtcgt ggctgtaatt gccacttttc cgggacaatc ggcaaatccg
    10381 caagattcca gcgcgcatag cggctgtcgt aggcgtctgg ctgcgcctgc tcaagcaggt
    10441 gaccgataca ccaagtcacc acctgaccat taccgcactc gataaagcca tcgcctttcc
    10501 ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag actcggtttt tcggcaataa
    10561 acaaccgcat tgaattaacg gatctcaacc atggcacgac cgccgcgcgc tggcaccagt
    10621 tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg tagttttaac ctcattttct
    10681 gcttccggca tgaccgccag cagcaaaccg ccagaagttt gcggatcgca cagcagatcg
    10741 cgcacttcac gcggcatttc acccatcaga tgaccgtagc tggcaaagtt acgttcagtg
    10801 ccgccaggta ctgcgcccaa cttaatgtac tcttcaacac cggggagttt cgggatcgct
    10861 tcatagtcga cgcgtgcctg cacaccagcc ccctgacaca tttcgctcaa gtggcccagc
    10921 agaccaaagc ccgtaacgtc ggtcatcgct tttacgcctt cgatgttggc aaaggacgcg
    10981 cctgcgatgt tcatccggca catcacttcc gtcgccagtc cctgatgttc tggtttcaac
    11041 agtgattttt tctcagccgt ggtaagaacg ccgatcccca gcggtttcgt caggaacagt
    11101 ttgcatccgg cttgtgcggt actgtttttc ttcacccgct cggtcggtac gatccccgtt
    11161 accgccagac caaaaatcgg ctccggcgca tcgatggagt gaccgccagc cagcgcaata
    11221 cccgcctgac ggcatgcata gcgtccacct tcggttactt cgcgggcaat ttccggggaa
    11281 agtttgttaa tcggccagcc gaggatcgca atcgccataa tcggtttgcc ccccatcgcg
    11341 aagatatcgc tgatggcgtt agtcgccgca atgcggccaa aatcgaaagg attatcaacg
    11401 atcggcataa agaagtcggt ggtactgata acgctggtgc cattgcccag atcgtacacc
    11461 gccgcatcgt cgcgggtttc attacccaca agcaaattcg gatcaacaaa cttcgcctgc
    11521 tcactgtgca ggatggtttc caacactttt ggggaaattt tacagccgca accagctccg
    11581 tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg acatctcctg tcaatgcaat
    11641 ccgggtatgg taaccctcat tccgtgaagt gataagtgag agtgtctgaa ttcctgcgcc
    11701 tttgctcaca atccagacag tttcgcgaca attatcagaa ataagttaca aacggcgtcg
    11761 ggtccgggac gttaatcgac gtagatgctt tcaactgcgg cgtgccgagg tagagaaaac
    11821 cgacaatttt atcctgctca cggcaaccga atgcttcacg cactaccgga ctttcagtta
    11881 atgcgccact gcgccagatg ccgccaaatc cctgggcaac ggctgccatt tgcatcgcca
    11941 tgaccgcgca tccggcagac atttcctgtt cccatcgcgg gactttatga ttttctttgc
    12001 atttcgccac caccgtgatg atgagcggtg cgcggaacgg cgcattacgg gctttgtcga
    12061 tagctttatc atcactaccg gcagcaatcg ccccctgttc cagtacggcg ctgaaacgct
    12121 cgcccccttc cccttcaatc acaaaaaaat gccacggttg catggactta tggtccggcg
    12181 cacgcatacc cgcacgcagg atgttttgca gttgttcacc cgttggcgcg ggttcagcca
    12241 ggcgggaggc gctacggcga ttgatcaata gttcgagtgc atccatttga ttaactcctg
    12301 tcgtgatatt tattcacaaa attaacacga gagtggattt tgttacagca cagtccgcaa
    12361 ttcctgctga caagtgccgg ttgggtcatt acgataacca catctattgc gcctgtgaca
    12421 ggtgtgacct taagttggga gaatacatgc gaaccctttg gcgatttatt gccggatttt
    12481 ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat ggtacttaac ctgttcttta
    12541 ttttcctcgt actggttggt gtggggatct ggatgcaggt cagtggtggt gattcgaaag
    12601 aaacggccag tcgtggcgca ctgctgctgg acatttctgg tgtgatcgtc gataaaccag
    12661 acagttctca gcggtttagt aaattaagcc gccagctgct tggtgccagt tccgatcgtc
    12721 tgcaggaaaa ctcactgttt gatatcgtca acaccattcg ccaggcgaag gacgaccgca
    12781 atatcaccgg tatcgtgatg gatctgaaaa acttcgcagg cggcgaccag ccgtctatgc
    12841 agtacatcgg caaagccctg aaagagtttc gtgacagcgg gaaaccggtt tatgccgttg
    12901 gcgagaacta cagccagggg caatattatc tcgccagttt cgccaataaa atttggctgt
    12961 caccgcaagg cgtggttgat ctgcacggtt ttgctaccaa cggtctgtac tacaaatcgt
    13021 tgctggataa gctgaaagtt tccccccatg tgttccgcgt gggtacgtat aaatctgccg
    13081 ttgaaccgtt tattcgtgat gatatgtcac cggcagcccg cgaagctgac agccgctgga
    13141 ttggtgagct gtggcaaaac tatctgaata ctgttgccgc taaccggcag atccctgctc
    13201 agcaggtatt ccctggcgcg caagggttgc ttgagggttt aaccaaaacc ggtggcgata
    13261 ccgcgaaata tgcactggaa aacaagctgg tcgatgcact ggcatcgagt gccgaaatcg
    13321 aaaaagctct gaccaaagag ttcggctgga gtaagactga taaaaattat cgcgccatca
    13381 gttattacga ttacgcattg aaaacgccgg cagataccgg tgacagcatc ggtgtcgtct
    13441 tcgctaatgg cgcaattatg gatggcgagg aaactcaggg gaatgttggc ggtgatacca
    13501 ctgcggcaca aatccgcgac gctcgccttg acccgaaagt gaaagcgatt gtcctgcgtg
    13561 ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat tcgcgctgaa ctggcagcag
    13621 cccgggcagc gggtaagtct gtggttgtat cgatgggcgg catggcggca tctggtggtt
    13681 actggatttc cacgccagct aattacattg tggctaaccc cagcaccctg accggttcta
    13741 tcggtatctt cggcgtgatc accaccgtag aaaatagtct ggattcgatt ggtgttcata
    13801 ctgatggtgt ctcaacttca ccgctggcgg atgtttctat caccagggca ctgccgccgg
    13861 aagcgcagca gatgatgcaa ttaagcattg agaatggcta taaacgcttt atcacgctgg
    13921 ttgctgatgc gcgtcattcg acgccggagc aaattgataa aatcgcccag ggccacgtct
    13981 ggaccggtca ggatgcaaaa gctaacgggc tggtcgatag tctcggggat ttcgatgatg
    14041 cggttgccaa agcagcagag ctcgcaaaag tgaaacagtg gcatctggaa tactacgttg
    14101 atgaaccgac cttcttcgac aaagtgatgg acaacatgtc tggttctgtc cgggcaatgt
    14161 tgccagatgc gttccaggcc atgttacctg caccgcttgc ctcggtagcc tctaccgtta
    14221 aaagtgaaag cgacaagctg gccgcgttta acgacccaca aaaccgttat gcgttttgcc
    14281 tgacctgcgc caacgtgcgt taagtcttgt actgagtggc cgacagatcg tcggccacat
    14341 tattttttac ctcgacgaat cctcttcccg ctgtctcgcc ccatatttcc ttatccgcat
    14401 agtatcaggt gcgctcctcc tgcctcacgt atatactttt gctctttcga tatcattcat
    14461 atcaatatca tgcaaaagaa atcaatttac gtcgcctaca cgggcgggac catcgggatg
    14521 cagcgttccg agcagggtta tattccggtg tcaggtcatc tacaacgcca actggcgctg
    14581 atgccggaat tccatcgccc ggagatgcca gatttcacca ttcatgaata tacgccgttg
    14641 atggattctt ctgatatgac gccggaagac tggcagcata ttgctgaaga tattaaagcg
    14701 cactatgacg actatgatgg ttttgtcatt ctacacggta ccgacacgat ggcgtatacc
    14761 gcctctgcgc tgtcgttcat gctcgagaat ctcggtaaac cggtcattgt gacagggtca
    14821 caaatcccgc tggctgagtt acgctctgac ggacaaatta atctgctgaa tgcgttgtac
    14881 gttgcggcga attatccgat caacgaagta acgctctttt tcaataaccg attgtatcgc
    14941 ggcaaccgca ctaccaaagc ccatgccgat ggttttgatg cgtttgcctc tccaaacctt
    15001 cctccgttac tggaagctgg tatccatatt cgtcgtttga atacgccacc cgccccgcac
    15061 ggtgaagggg aattaatcgt tcatccaatc acgccacaac caattggcgt agtgacgatt
    15121 tatccaggga tttctgctga cgtcgtgcgc aattttctgc gccaaccggt gaaagcattg
    15181 attctgcgct cctatggcgt gggtaatgcg tcacaaaaca aagccttcct gcaggaatta
    15241 caagaagcca gcgatcgcgg tattgtggtg gtcaacctga cacaatgtat gtccggtaaa
    15301 gtgaatatga gtggttatgc caccggtaac gccctcgccc atgccggcgt tattggcggt
    15361 gcagatatga ctgtagaagc cacgctaacc aaactgcatt acctgctgag ccaggaactg
    15421 gatactgaaa ccattcgcaa ggccatgagc caaaacctgc gcggcgaact gacgccggat
    15481 gattaaggag actgcaatgc cccatcgcgc cctgttactg gtcgatttac aaaatgattt
    15541 ctgtgctggt ggcgcgctcg ccgtaccgga aggtgacagt acggtggagg ttgctaaccg
    15601 ccttattgac tggtgccagt cgcgcggaga agcggttatc gccagccagg actggcat
//
LOCUS       DADLSB010000093        15648 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.093, whole genome shotgun sequence.
ACCESSION   DADLSB010000093 DADLSB010000000
VERSION     DADLSB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.093"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(95..202)
                     /gene="rprA"
                     /locus_tag="JN331_002707"
     ncRNA           complement(95..202)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JN331_002707"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(388..1500)
                     /gene="ydiK"
                     /locus_tag="JN331_002708"
     CDS             complement(388..1500)
                     /gene="ydiK"
                     /locus_tag="JN331_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY7260821.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1889..4945
                     /locus_tag="JN331_002709"
     CDS             1889..4945
                     /locus_tag="JN331_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7260822.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGIVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4942..5352
                     /gene="menI"
                     /locus_tag="JN331_002710"
     CDS             4942..5352
                     /gene="menI"
                     /locus_tag="JN331_002710"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY7260823.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5452..5640
                     /locus_tag="JN331_002711"
     CDS             5452..5640
                     /locus_tag="JN331_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260824.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6188..6556
                     /gene="sufA"
                     /locus_tag="JN331_002712"
     CDS             6188..6556
                     /gene="sufA"
                     /locus_tag="JN331_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY7260825.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6565..8052
                     /gene="sufB"
                     /locus_tag="JN331_002713"
     CDS             6565..8052
                     /gene="sufB"
                     /locus_tag="JN331_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY7260826.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKSVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEQSVG"
     gene            8062..8808
                     /gene="sufC"
                     /locus_tag="JN331_002714"
     CDS             8062..8808
                     /gene="sufC"
                     /locus_tag="JN331_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY7260827.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8783..10054
                     /gene="sufD"
                     /locus_tag="JN331_002715"
     CDS             8783..10054
                     /gene="sufD"
                     /locus_tag="JN331_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY7260828.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTESLRDEGLKQQVLARIGQRLPGGAR"
     gene            10051..11271
                     /gene="sufS"
                     /locus_tag="JN331_002716"
     CDS             10051..11271
                     /gene="sufS"
                     /locus_tag="JN331_002716"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY7260829.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11284..11700
                     /gene="sufE"
                     /locus_tag="JN331_002717"
     CDS             11284..11700
                     /gene="sufE"
                     /locus_tag="JN331_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY7260830.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11849..12853
                     /gene="ldtE"
                     /locus_tag="JN331_002718"
     CDS             11849..12853
                     /gene="ldtE"
                     /locus_tag="JN331_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY7260831.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12917..13153)
                     /locus_tag="JN331_002719"
     CDS             complement(12917..13153)
                     /locus_tag="JN331_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY7260832.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13464..14876)
                     /gene="pykF"
                     /locus_tag="JN331_002720"
     CDS             complement(13464..14876)
                     /gene="pykF"
                     /locus_tag="JN331_002720"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY7260833.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     NATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15433..15642
                     /gene="fumD"
                     /locus_tag="JN331_002721"
     CDS             15433..15642
                     /gene="fumD"
                     /locus_tag="JN331_002721"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAY7260834.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3971 a         3770 c         4145 g         3762 t
ORIGIN      
        1 cattatcagc ctgctgacgg cttgaagaga gtcacagtat cttgtgcaac attattggaa
       61 agagtgaggg gcgaggtagc gaagcggaaa aatgttaaaa aaaagcccat cgtgggagat
      121 gggcaaagac tacacacagc aattcgttgt ttcactcagg ggatttccat gcttataaat
      181 caatatgttg atttataacc gtgagctaat agtaggcata cggacttttt gcgtcgatca
      241 gattcgtctc attagtttaa aaactgtaaa gaatttgcga caggtactac cagctgaatg
      301 gcggtaagca ggaaaatatg gggaagagca agcaaatatc aattagcggt gattaacaat
      361 caggcggcag agccgcctga tgagaaatta cttattcggt ttctcgattt cgccaagttc
      421 ttcgagaatt tcttccggtt ggtccgtcgg cggcgggact tcttccaccc acgcggcaaa
      481 cagacgccag gaaacggcta acagaaccgg accaataaac agaccgatca tcccgaaagc
      541 aatcaaacca ccaataacgc cagagaggat caggatcagc ggtaaatcgg cacccatgcg
      601 aattaacatt gggcggatga cgttatccag cgtgccaacc acaccgctcc acactaacaa
      661 taccgttccc caggtggtat cgccggtcca gtagagccag ataatcgccg gaatcagtac
      721 cggcaacggg ccaagctgga caaggcagga gaggatcatt aacaccgtta gcaacgttgc
      781 ataaggtacg ccggatacgg cgaggccgat accgccaagc actgcctgta ctaacgccgt
      841 caccaccaca cccagcgcca ccgcgcggat agcctgtgcc gccagcagca cggcggcatc
      901 tccgcgaacg cctgccagac gggttgcaaa atggcgaatg ccttgtgcca cctgttcacc
      961 gcgccagtac agcagggcac tgaagagaag catcagcgca caatgcacca taaagcgccc
     1021 gatatgcgcc gcctgcccaa cgaaccaggt ggtggtggtg ccaatataag ggcggacttt
     1081 cgccatgatc gccgtacccc ccatatccag caagttgtgc cagcctgcat acagcttcgc
     1141 gccaatcacc ggaatggtat taagccacgc cagatcgggt aacgtcatgt cgccgctgga
     1201 aatggcttta attagcgggc cgctgccgtc gacgatactg ttaaccagca aagcaatagg
     1261 gatgataaac accatcacta ataacagcgt catcaccaga acggcgagag agcggcggcc
     1321 aaacatgatc ttttgcaaac gtaacaatac cggccaggtg gcgataacca ccgtaccggc
     1381 ccatgcaaag ccgagaataa agggttgaac aatccacaga catgccacaa tcatgatggc
     1441 taaaaacagc accgaaagca gaatttgtgc gacatccctg ggctgacgaa catttaccat
     1501 aactactttt cacctttttt gtgcgtcaaa tcgtcgacgc gacgtgaaca cgcgaaactc
     1561 acctgcataa tgatgagcaa tttcagcggt ttttaacagg ccgattctgc ccttaattct
     1621 gatgggcgtg ttagaaaaaa atgtggtaca acaactcagg caacacgcaa acgatttact
     1681 cgtcgtattt caagttgcat gtgctgcggc tacgttcgtt caccccagtc acttactatt
     1741 gtaagctcct ggagttcact cgcttgcagc cttcctgcaa ctcgaaatcc atagagtaaa
     1801 taaacatcac ctttcagtgc gctattgcag ccgcctgaaa gaatagtgta tcgacctaca
     1861 tcacagaccg caggaaaggg tcaatataat gattccacag atttcccagg cacccggcgt
     1921 cgttcaactg gtgcttaatt ttttgcaaga gctggagcaa caaggtttta ccggcgatgc
     1981 ggcgacaagt tatgccgatc gtctgacaat gtcgaccgac aacagtattt accaacttct
     2041 ccccgatgcg gtggtatttc cgcgttcaac cgcagatgtg gcgctgatcg cccgtcttgc
     2101 cgcgcaggaa cgctattcat cgctgatctt taccccccgc ggcggcggca ccggcactaa
     2161 cggtcaggcg ctcaaccagg ggattattgt tgatatgtcc cgccatatga accgcatcat
     2221 cgaaattaac cctgaagagg gctgggtgcg cgttgaggcc ggggtgataa aagaccaact
     2281 caatcagtac ctgaaaccgt tcggctactt ttttgcgccg gaactttcga ccagcaaccg
     2341 ggcaacgctc ggtgggatga tcaataccga tgcatccggt cagggatcgc tggtctatgg
     2401 caaaacgtca gatcacgtac ttggcgtacg cgcggtgttg ttggggggcg atattctcga
     2461 tacgcaacct ttacccgtcg agctggcgga aacgctgggt aaatccaata ccacaatcgg
     2521 gcgaatttat aacacggttt atcaacgttg ccgtcagcaa cgccagttaa tcatcgacaa
     2581 cttccccaaa cttaaccgct ttcttaccgg ttacgatctg cgtcatgtct ttaacgatga
     2641 gatgaccgag ttcgacctga cgcgcattct gacgggttca gaagggacgc tggcctttat
     2701 taccgaagcg cggctggata ttacgcgctt gcctaaagtg cgccgtctgg tgaatgtcaa
     2761 atatgactct tttgactctg cgctgcgtaa cgcgccgttt atggttgagg cgcgggcgct
     2821 ttcggtagag acggtggact caaaagtgct gaatctggcg cgggaagata ttgtctggca
     2881 ttccgtcagc gagttgatta ccgatgtgcc tgacaaagag atgctcggac tgaacattgt
     2941 ggaatttgct ggtgatgatg aggcgctgat tgacgagcga gttaatgcac tctgtgtgcg
     3001 gcttgatgag ctgattgcca gccaacaggc aggtgtgatt ggctggcagg tgtgccgcga
     3061 gctggcgggg gttgaacgta tctatgcgat gcgcaaaaaa gccgttggtc tgcttggcaa
     3121 tgccaaaggt gccgctaagc caattccgtt tgctgaggat acctgcgtac cgccggaaca
     3181 tctggccgat tatattgctg aatttcgcgc gctgctcgac agccacggct taagctacgg
     3241 tatgttcggt cacgtcgacg caggtgtctt gcacgtccgt ccggcactgg atatgtgcga
     3301 tccgcaacaa gagattttga tgaagcaaat ctctgatgac gtggtggcgc tgactgcgaa
     3361 atacggtggt ttgttgtggg gcgagcacgg caaaggtttt cgcgctgaat acagcccggc
     3421 gtttttcggt gaggaacttt ttgcagaact gcgcaaagtg aaagcggcat ttgacccgca
     3481 taaccgactc aacccaggga agatttgccc gccagaaggt ctcgatgcgc cgatgatgaa
     3541 agtggacgcg gtgaagcgcg gtacattcga tcggcagatc cccattgcgg tacgccagca
     3601 gtggcgcggt gcgatggagt gtaacggcaa cggtttatgc ttcaactttg atgcccgtag
     3661 tccgatgtgt ccgtcgatga agatcaccca gaaccggatt cattcaccga aagggcgcgc
     3721 aacgctggtg cgtgaatggc tgcgcttgtt ggcggatcgc ggcgttgacc cactcaaact
     3781 ggaacaagaa ctgcctgaat ccggcgtcag tttgcggacg ttaattgccc gcacgcgcaa
     3841 tagctggcat gcgaataaag gcgaatatga cttctcacac gaagtcaaag aggcgatgtc
     3901 gggctgtctg gcctgtaaag cttgttcgac ccagtgcccc atcaaaattg atgtgccgga
     3961 gtttcgctct cgttttctgc agctctatca cacccgttat ttacgcccgc ttcgcgacca
     4021 cctcgttgct acggtcgaga gctacgcacc gctgatggca cgcgcgccga aaacctttaa
     4081 cttcttcatt aaccagccgc tggtgcgcaa actctcggaa aaacatatcg gcatcgttga
     4141 tttgccactg ctgtcggtcc cctcgctaca acaacaaatg gtggggcatc gctcggcaaa
     4201 catgacgctg gaacagcttg aagctctcaa tgcagagcag aaagcgcgca cggtgttggt
     4261 ggtgcaggac ccctttacca gctattacga tgcgcaagtg gtggcggatt ttgtccgtct
     4321 ggttgaaaaa ttaggtttcc agcctgtgtt actgccattt tcgccaaatg gcaaagccca
     4381 gcatatcaaa ggctttctta atcgttttgc gaagacggcg aaaaagacgg cggatttcct
     4441 caaccgtatg gctaagcttg ggatgccaat ggtgggcgtc gatccggcgc tggtactttg
     4501 ttatcgcgat gaatataaac tggcgctggg cgaggaacgt ggcgagttta acgtcttact
     4561 tgcgaatgaa tggctggcaa gcgcacttga gtcacatcca gtggctacag tcagcggtga
     4621 atcatggtat ttctttggtc actgtaccga ggttaccgct ttgccgggcg cgccagcaca
     4681 atgggccgcg atatttgccc gttttggcgc gaaactggaa aatgtcagcg tgggttgctg
     4741 cggcatggca gggacttacg gacatgaagc gaaaaaccat gaaaattcgc tcgggatcta
     4801 tgagttatcc tggcatcagg ctatgcagcg actgccgcgt aaccgctgtc tggcgaccgg
     4861 atattcctgc cgtagccagg taaaacgggt tgaaggcacg ggggtacgcc atcctgtgca
     4921 ggctttactg gagattatta aatgatatgg aaacggaaaa tcaccctgga agcactgaat
     4981 gctatgggtg aaggaaacat ggtggggttc ctggatattc gctttgaaca tattggtgat
     5041 gacacccttg aagcgacaat gccagtagac tcgcggacaa agcagccttt cgggttgctg
     5101 catggaggag catccgtggt actggccgaa agtatcggtt ccgttgccgg ttatttatgt
     5161 accgaaggtg agcaaaaagt ggttggtctg gaaatcaatg ctaatcacgt ccgctcggca
     5221 cgagaagggc gggtgcgcgg catatgtaaa ccgttgcatc tcggttcgcg tcaccaggtc
     5281 tggcagattg aaatcttcga tgagaaaggg cgtttgtgct gttcgtcacg attgacgacc
     5341 gccattttgt gacgttgtca ccagaaaagt gtgacgcata ttgcttttgg tcattatctg
     5401 cacagtaaac ggtggtatac tgtgcaggtc ttgtctaaaa gcgaggatgc tatgtctact
     5461 caacttgatc ccacccagct ggccattgaa tttttacgcc gtgaccagag caatctttca
     5521 cctgcccaat atctcaaacg tctgaaacaa ctagaacttg aatttgctga cctgcttact
     5581 ctctcttctg ctgaattaaa agaagaaatc tactttgcct ggcgtctggg cgtgcattga
     5641 tcctgactta atgccgcaag ttcgccaaat cgacctggat taacctccgt ccaggttcga
     5701 tgaccttctt aatactgctt atctaccgct tgatattgtt caaaaataat actaatcgca
     5761 gtttgtgtta aaacggcggg ttagctttat gagattattc ttatcgcccc ttcaagagct
     5821 aagccactga gagtgccgga gataagcgcc ggatggggta gaaaccctta agcctgtgtc
     5881 gcacagactt aagggttttc ttatttctga acgctgcact tttttaccta tctcttaaat
     5941 agtcattttc aatacaactt tttctttttc atcacagcat taatgctcct tagataataa
     6001 ccattatcta acaatgagat acctaattct tagcgtgcct gttaacccac acatcagggg
     6061 ctatgcttat taaaagtagg caaaaagcat gttttacggt aaagcccctg cgtttgctgg
     6121 gttgaactga taatcattat cactaacatg ctgttatacg ctgaaagcga tgaagtgagg
     6181 taaatagatg gacatgcatt caggaacctt taacccacag gatttcgcct ggcaaggctt
     6241 aacgctgaca cccgcagcgg cgatacacat ccgcgagctg gtggcaaagc agccaggtat
     6301 ggtcggcgtg cgcttaggtg tgaagcaaac gggctgcgcg ggctttggct atgtgctcga
     6361 cagtgttagc gagccggaca aagacgatct gctgtttgaa cacgacggcg cgaagctgtt
     6421 tgtcccgctg caatcgatgc cgtttattga tggcacggaa gtcgatttcg ttcgtgaagg
     6481 acttaatcag atattcaaat ttcacaaccc taaagcccag aatgaatgtg gctgtggcga
     6541 aagctttggg gtataggcgg tactatgtct cgtaatactg aagcaactga cgatgtcaaa
     6601 acctggaccg gcggcccgct gaattataaa gaaggattct tcacccagtt agccaccgat
     6661 gagctggcaa aggggataaa cgaagaggtg gtgcgcgcaa tttcggcgaa gcgtaatgag
     6721 ccggagtgga tgctggagtt tcgtctaaac gcctatcgcg catggctgga gatggaagaa
     6781 ccgcactggt tgaaagcgca ctacgacaag ctgaattatc aggattacag ctactactca
     6841 gcaccatcgt gcggtaattg tgacgacact tgcgcgtctg aacctggcgc ggtgcagcaa
     6901 actggcgcga acgccttttt aagtaaagag gtggaggcgg cgtttgagca gttgggcgtt
     6961 cccgtgcggg aaggcaaaga ggtggcggtg gatgccattt tcgactctgt ttcggttgcc
     7021 accacttatc gcgaaaaact ggcggagcag ggaattattt tctgttcctt tggtgaggcg
     7081 atccacgatc acccggaact ggtgcgtaaa tatctcggca ccgtggtgcc ggggaatgac
     7141 aacttctttg cagcacttaa tgcggcggta gcctctgacg gtacgtttat ttatgtgcct
     7201 aaaagcgtgc gctgcccgat ggaactttcc acctattttc gcattaacgc ggaaaaaacc
     7261 gggcagtttg agcgcacaat tctggtggcc gacgaagaca gctacgtcag ctacattgaa
     7321 ggctgttccg ctccggtgcg tgacagctat cagttgcacg cggcagtggt ggaagtcatc
     7381 atccataaaa acgccgaggt gaaatattcc acggtacaaa actggttccc tggcgataac
     7441 aacaccggcg gtattctcaa cttcgtcacc aagcgtgctt tgtgcgaagg cgaaaacagc
     7501 aaaatgtcat ggacgcaatc agaaaccggg tcagcgatta cgtggaaata tcccagctgc
     7561 attttgcgcg gcgataactc cattggtgag ttttactcag tggcgctgac cagcggtcat
     7621 cagcaagcgg ataccggcac caagatgatc cacatcggta aaaacaccaa atcgaccatt
     7681 atctcgaaag ggatctctgc cggacatagt cagaacagtt atcgcggctt agtgaaaatc
     7741 atgccgacgg caaccaatgc gcgcaatttc actcagtgcg actcaatgct gattggcgct
     7801 aattgtgggg cgcatacctt cccgtatgtc gaatgtcgca ataacagcgc acaactggag
     7861 cacgaggcaa cgacatcacg tattggtgaa gatcaactgt tttactgcct gcaacgcggg
     7921 atcagcgaag aagacgccat ctcgatgatt gttaacggtt tctgcaaaga cgtgttctca
     7981 gaactgccgc tggaatttgc cgttgaagca caaaaactcc tcgccatcag tcttgaacaa
     8041 agcgtcggat aaggaataaa catgttaagt attaaagatt tacacgtcag cgtggaagat
     8101 aaagctatcc tgcgtggatt aagcctcgac gttcgtcccg gcgaagttca cgccattatg
     8161 gggccaaacg gttcgggcaa aagtacctta tcggcaacgc ttgccgggcg cgaagattat
     8221 gaagtgacgg gcggcacggt tgagttcaaa ggcaaagatt tgcttgcact gtcgccggaa
     8281 gatcgtgcag gcgaaggcat ctttatggcc ttccagtatc cggtggagat tccaggcgtc
     8341 agtaaccagt ttttcctgca aacggcactt aatgcagtgc gcagctatcg cgggcaggaa
     8401 acgctcgacc gctttgattt tcaggatttg atggaagaga aaatcgctct cctgaagatg
     8461 ccggaagatt tattaacccg ttcggtaaac gttggttttt ccggcggcga gaaaaagcgc
     8521 aacgatattt tgcaaatggc ggtgctggaa ccggagttat gcattcttga tgagtcggac
     8581 tccgggctgg atattgacgc attaaaagtg gtcgccgatg gcgtgaactc gctgcgtgat
     8641 ggcaagcgct cattcatcat tgttacgcac taccaacgca ttctcgacta catcaagcct
     8701 gattacgttc atgtgctata tcagggacga attgtgaaat ccggcgattt cacgttggtc
     8761 aaacaactgg aggagcaggg ttatggctgg cttaccgaac agcagtaatg cgctgcaaca
     8821 gtggcatcac ttgtttgaag ctgaagggac aaaacgctca ccgcaagcac agcagcattt
     8881 acaacaattg ctgcgtaccg gactgccgac acgtaaacat gaaaactgga aatatacgcc
     8941 gctggaaggg ctgaccaata gtcagtttgt cagcattgcg ggagatatat ccccacagca
     9001 gcgtgatgcc ttagcgttaa cgttagacgc cgtgcggctg gtgtttgtcg atggacgtta
     9061 cgtgcccgcg ctgagcgatg caattgaagg cagcggatat gaagtgagca ttaacgacga
     9121 ccgtcagggg ttacccgacg ctattcaggc ggaagtgttt ctgcatttga cggaaagcct
     9181 ggcacaaagc gtgacgcata tcgccgtgaa gcgcggtcaa cggccggcaa agccattgct
     9241 gttaatgcat atcacccagg gcgtggcagg tgaagaggtg aacactgccc attaccggca
     9301 tcatctggat ctggcggaag gtgccgaagc aacggtgatc gaacattttg tcagccttaa
     9361 tgatgctcgt cattttaccg gggcacggtt cactatcaat gtcgcagcga acgcccactt
     9421 gcagcatatc aagctggcgt ttgaaaaccc ggtcagtcac cactttgccc ataacgattt
     9481 gttgctggct gacgatgcca ccgcatttag ccatagtttc ctgctgggtg gcgcagtgtt
     9541 acgacacaac accagtacgc aactcaatgg cgaaaacagc acgctgcgga tcaatagcct
     9601 ggcgatgccg gtgaaaaacg aggtgtgtga tacccgtacc tggctggaac acaataaagg
     9661 tttttgtaac agccgacagt tgcacaaaac tatcgtcagc gacaaaggcc gcgcggtatt
     9721 taacggtttg atcaacgtcg cgcagcacgc catcaaaacg gatggtcaga tgaccaacaa
     9781 taatctgctg atgggtaaac tggcggaagt ggatacaaaa ccgcagctgg aaatctatgc
     9841 agatgatgtg aaatgcagcc acggcgcgac ggtggggcgt attgatgatg aacagatgtt
     9901 ctatctgcgc tcgcgcggga tcaatcagca ggatgcccag cagatgatca tttacgcctt
     9961 tgctgccgaa ctgacggaat cactgcgtga tgaggggctt aaacagcagg tgctggcccg
    10021 aatcggtcaa cggctgccag gaggtgcaag atgacttttt ccgtcgacaa agtgcgggcc
    10081 gactttccgg tgctttcgcg tgaggtaaac ggtttgccgc tggcttatct cgacagcgcc
    10141 gccagtgcac agaaaccgag ccaggtgatt gacgccgagg ccgagtttta tcgtcatggc
    10201 tacgcggcgg tgcatcgcgg tattcatacc ttaagcgccc aggcgaccga gaaaatggag
    10261 aacgtgcgca agcgggcatc gctgtttatt aatgcccgtt cggcggaaga gctggtgttc
    10321 gtccgcggca cgacggaagg gatcaatctg gtcgccaata gctggggcaa cagcaatgtg
    10381 cgggcgggcg ataacatcat catcagtcag atggagcacc acgctaacat tgtcccctgg
    10441 cagatgcttt gcgcacgcgt tggcgcagag ctgcgtgtga tccctctcaa ccccgacggt
    10501 acgctgcaac tggagacgct gcctacgctg tttgatgaga aaactcgcct gctggcaatt
    10561 actcatgtct ccaacgtgct tggcacagaa aatccactgg cggaaatgat cacgcttgcg
    10621 caccagcatg gcgcaaaagt gctggtggat ggcgctcagg cggtgatgca tcatccggtg
    10681 gatgttcagg cgctggattg cgacttttac gtgttctccg ggcataaact gtatggcccc
    10741 accggaattg gcattcttta tgtgaaagaa gccttgttgc aggagatgcc gccgtgggaa
    10801 gggggcggtt ctatgatcgc caccgtcagc ctgagtgaag gcactacctg gaccaaagca
    10861 ccatggcggt ttgaagccgg tacacccaat accgggggca tcattggtct tggcgcggcg
    10921 ctggagtatg tttcggcgct ggggcttaat agcatagccg agtatgaaca gaatctgatg
    10981 cattatgcgc tatcacagct ggaatctgta ccggatctca ctctctatgg cccacaaaac
    11041 aggcttggcg ttattgcttt taatctcggt aaacaccacg cctatgatgt tggcagtttt
    11101 ctcgataatt acggcattgc tgtgcgtacc ggacatcact gcgctatgcc attaatggcc
    11161 tattacaacg tccctgcgat gtgtcgggcg tcgctggcca tgtataacac ccatgaagaa
    11221 gtggatcgtc tggtgaccgg cctgcaacgt attcaccgtc tgctgggata acagggaggc
    11281 actatggctt tattgccgga taaagaaaaa ttgctgcgta attttttacg ctgcgccaac
    11341 tgggaagaga aatatctcta cattattgag ctgggccagc gtctgccaga attacgcgcc
    11401 gaagaccgaa gcccacaaaa tagcattcag ggctgccaga gtcaggtgtg gattgtcatg
    11461 cgccagaatg cgcagggaat tattgaattg cagggcgaca gcgatgcggc gattgtgaaa
    11521 gggcttattg cggtcgtctt tattctctac gatcagatga cgccgcagga tattgtcaat
    11581 ttcgatgtgc gtccgtggtt tgaaaaaatg gcgctcaccc aacatctcac cccatctcgt
    11641 tcacaaggtc tggaagcgat gattcgcgca attcgcgcca aagccgctgc acttagctaa
    11701 actaaaggga cagctttcat ccggttggct cacattgccg gatggtcaat gtttaaactg
    11761 acctgcacag gagtgttttg gtttcaggtg agcataagcg ccatccgacg tttcagcgtg
    11821 agtctccggc aaatacagga ggtttacaat gaaacgcgcg tctttgctta cactcacgct
    11881 tatcggcgct tttagcgcca tccaggctgc ctgggcggtt gattatccgc taccaccaac
    11941 cggaagccga ctggttgggc aaaatcaaac gtatacggtg caagaagggg ataaaaacct
    12001 tcaggctatt gcccgacgtt ttgatactgc ggcaatgttg atccttgaag ccaataacac
    12061 tatcgccccg gtgccaaaac ctggtacgac gataactatt ccttcacaac tgttattacc
    12121 tgatgcaccg cgtcagggga ttatcgttaa ccttgcagag ctgcgccttt attattatcc
    12181 gccgggagaa aatattgtgc aggtttatcc aataggtatt ggattgcagg ggctggaaac
    12241 gccggtgatg gaaacgcgtg ttgggcagaa aatccctaac ccaacctgga cgcctacggc
    12301 aggcattcgt cagcgttcgc tggagcgtgg cattaaatta ccgccagtcg ttcctgccgg
    12361 accaaataac ccgctaggac gttacgcact gcgcctcgcg catggtaatg gcgaatacct
    12421 cattcatggc accagtgcgc cggacagcgt tggtttgcgc gtcagttcag ggtgtattcg
    12481 catgaatgca ccggatatta aagccttgtt ctccagcgtg cggacgggaa cgccggtgaa
    12541 agtgatcaac gaaccggtga aatattccgt ggaacctaac gggatgcgtt atgttgaagt
    12601 acatcgacca ctatcagcag aagaacagca aaacgttcag acaatgccat acacactgcc
    12661 agcaggcttt acgcaattta aagacaataa ggctgtagat cagaagttag tcgataaagc
    12721 gttgtatcgt cgggcagggt atccggtttc ggtgagcagt ggagcaactc ccgcagccag
    12781 caatgcgcct tcagtagagt cagcgcagaa tggtgaacca gagcaaggga atatgttacg
    12841 cgcgacgcag tagtggtaaa tagcagacaa aaaaaatggc gcacaatgtg cgccattttt
    12901 cacttcacag gtactattac ttgcggtatt tagtagccat gttgtccaga cgctggttag
    12961 cacgagctgc gtcatcttta gcagcctgaa cgtcggaacg cattgcgttc acgtcgttgc
    13021 tcagctggtc aactttagcg ttcagagtct gaacgtcaga agacagctga tcgattttag
    13081 cgttgctgga gcaacctgcc agcagagtag aacccaggat taccgcgccc agtaccagtt
    13141 tagtagcttt cattattaat accctctaga ttgagttaat ctccatgtag cgttacaagt
    13201 attacacaaa gttttttatg ttgagaatat ttttttgatg ggaaggcact tatttttgaa
    13261 cgttcgctca aagaagcatc gaacgctggg ttttagataa aaaaattgag agaaaacagg
    13321 atgcttccat cggattcatc ttagataaag tgagattata tagtgaaatc cgatttgatt
    13381 ttttattgct tctggttatc gattaaataa aaaaagcgcc catcagggcg cttcgatata
    13441 caaattaatt cacaaaagca atattacagg acgtgaacag atgcggtgtt agtagtgccg
    13501 ctcggtacca gtgcaccaga aaccataact acgacgtcac ctttgtgtgc cagaccgctc
    13561 tgcagagcca gttctttacc cagacggtag aaatcatcag tagaagtgat ctctttaacc
    13621 agctgcggca caacgccttt gctcagtacc aactgatgag ccgttttttc gttggtggtc
    13681 agtgccagga tggtggcatt cgggaagtat ttacgtactg cgcgagcaga tttaccgccc
    13741 tgggtagcaa ccacgatcag cggagcatcc agtttttcag cagtttcaac ggcaccacgg
    13801 cataccgctt cggtgatgcg cagtttacgg ttgtcattgt tgaactcgag acggctgttc
    13861 atcacgcggt cggtacgttc gcagatggtc gccatgatag aaaccgcttc cagcgggtat
    13921 ttaccttttg cggattcacc agacagcatc actgcgtcag taccgtcgag gatggcgttt
    13981 gcaacgtcac cggcttctgc gcgagtcggg cgtgggtttt tgatcatgga atccagcatc
    14041 tgggtcgcag tgataacgac tttacgtgca cggatacatt tttcgatcat catcttctgg
    14101 gcgaagataa cttcttctac cgggatttct acacctaggt cgccacgcgc aaccatgatg
    14161 ccgtcagagg cttcgaggat ttcgtcgaag ttgttgaggc cttcctggtt ttcgattttg
    14221 gagatgatgt ggatgttttc gccgccgtgc gctttcaggt gctcacggat ttcgataacg
    14281 tcagaacgct tacgaataaa ggaagcagca acaaagtcta cgcctttttc gcaaccaaag
    14341 atcaggtcct gtttgtcttt ttcagccagt gctggcagag caatggaaac gccaggcagg
    14401 ttcacacctt tgttttcgcc caggtcaccg ttgttcagca ctttacagat aactttgtta
    14461 ccttcaatgg cggtaacttc cataccgatc agaccatcgt caaccagtac ggtgttgcca
    14521 acagacaggt cagtagtgaa accttcatac gttaccgcaa ccatttcgct gttgccgata
    14581 acagatttat cagtggtgaa agtaaaggtc tgaccagctt tcagagaaac atcgttaccg
    14641 ccttccagtt tcatggtgcg gatttccgga cctttggtat caagcaggat agcggcggtt
    14701 ttaccagttt tgctcatcac gttgcgcaga ttctgaatgc gctgaccgtg ttctgcatag
    14761 tcaccatgag agaagttcag acgcataacg ttcatgccag cgtccagcat tttagctaac
    14821 atctcttcag attcggtttt cggtccgatg gtgcaaacaa ttttggtctt tttcatgaca
    14881 gtcttagtct ttaagttgag aaggatggga gaaacttgct ttctgggcgc actgccggaa
    14941 agcgaatccg gtattacgaa agtggtggtg cctcgctcta aggataggtg acatcgaaaa
    15001 agcgtgcaga ggaatgtgtg cttgtgtctc agcccaacgt gagtgcctgt ttttgagtcg
    15061 ttgagttcta cagtccaaag tgctgaaacc attcaagagt caattggcgc gcattatacg
    15121 ctgaaattaa tccaaaagga aaacaaaaac agcgtttcaa aaaatataca acttgttaga
    15181 caataaattg ttatcaaaga tttaacgttc cagggaaaag gttacgcaaa aacttcgctg
    15241 tttgcgcaac gtctggtgcc gaagttgtca gtgtgaggat aagtgaaagt tcatccggtg
    15301 tataaatata tcatcgctca attgctacag ttaaaataat ttatcgcgtt atttgtctga
    15361 ttttgtttga tttttgttta attggttaga ttgatatatg ctgatagttg gcatgataaa
    15421 taggtataaa gaatgggaaa tcggacaaaa gaggatgaac tgtaccgaga aatgtgcaga
    15481 gttgtcggta aagttgtgct ggaaatgcgc gatctggggc aggaaccaaa gcatattgtt
    15541 atcgcaggcg tattacgtac agcattagcc aataaacgca ttcagcgcag tgagctggaa
    15601 aagcaggcga tggaaaccgt cattaacgca ctggtaaaat gaccattc
//
LOCUS       DADLSB010000094        15386 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.094, whole genome shotgun sequence.
ACCESSION   DADLSB010000094 DADLSB010000000
VERSION     DADLSB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.094"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            455..2734
                     /gene="ydeP"
                     /locus_tag="JN331_002722"
     CDS             455..2734
                     /gene="ydeP"
                     /locus_tag="JN331_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY7260835.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2982..3179
                     /gene="safA"
                     /locus_tag="JN331_002723"
     CDS             2982..3179
                     /gene="safA"
                     /locus_tag="JN331_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY7260836.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3253..4014
                     /gene="ydeO"
                     /locus_tag="JN331_002724"
     CDS             3253..4014
                     /gene="ydeO"
                     /locus_tag="JN331_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY7260837.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4416..6098
                     /locus_tag="JN331_002725"
     CDS             4416..6098
                     /locus_tag="JN331_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7260838.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6150..7307
                     /locus_tag="JN331_002726"
     CDS             6150..7307
                     /locus_tag="JN331_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY7260839.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7273..7395)
                     /locus_tag="JN331_002727"
     CDS             complement(7273..7395)
                     /locus_tag="JN331_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260840.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            7604..9289
                     /locus_tag="JN331_002728"
     CDS             7604..9289
                     /locus_tag="JN331_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY7260841.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGIWNLADDICDISTVL"
     gene            9327..11699
                     /locus_tag="JN331_002729"
     CDS             9327..11699
                     /locus_tag="JN331_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY7260842.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANDSDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            11744..14539
                     /locus_tag="JN331_002730"
     CDS             11744..14539
                     /locus_tag="JN331_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7260843.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETF
                     ESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAADQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            14901..>15386
                     /locus_tag="JN331_002731"
     CDS             14901..>15386
                     /locus_tag="JN331_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7260844.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PV"
BASE COUNT         4501 a         3362 c         3367 g         4156 t
ORIGIN      
        1 ggggacagta aataaaagca aagtacctct gggtttaagc gcaacctatg gacaaacagg
       61 aaacaaggtt tcagccggaa ccgtccagtc agtcattggt gtcactttta tctatgaata
      121 attgtgattt tgaccttagt ctatgcgctc cattcacgaa aattgcgcgg ataattattt
      181 tgtgaaggct attatcctac acctgtaaga aaatccgcgc acaattgaag ccagagtctt
      241 attttttata ttgaaaaata tgttaattta aacactatga gagatgatgc ttaacttatc
      301 gtatttaatc tattgtttaa cgataataga ttaaaaatag aaagagagta gtcccctact
      361 cttatatatc catgttggcg ataatccctt tgtatgtact gtgcatcatc gctattacaa
      421 atcctaataa ttcatttcca cacaggataa gtagatgaag aaaaaaattg aatcctacca
      481 gggtgctgca ggtggttggg gtgctgttaa atccgtagcg aatgcagtac gtaagcagat
      541 ggatatacgc catgatgtta ttgccatgtt tgacatgaat aagccagagg gctttgactg
      601 tccgggttgt gcatggccag atcctaagca cagtgcgtca ttcgacattt gtgaaaacgg
      661 cgcaaaagca atcgcctggg aagtcacgga taagcaggta aatgcctctt tctttgctga
      721 gaatacggtt caatcattac ttacctgggg agatcacgag cttgaggctg cggggcgtct
      781 cactcagcct ttgaaatatg atgccgtcag cgactgttac aagccattaa gctggcaaca
      841 agctttcgac gaaattggcg cacgccttca aagctatagt gatcccaatc aggttgaatt
      901 ctatacttcg ggccgcactt ccaatgaagc tgcctttctt tatcagcttt ttgcccgtga
      961 atacgggagc aataactttc ccgactgctc caacatgtgc catgaaccga caagcgtggg
     1021 tttggcagcg agtatcggtg taggtaaagg gaccgtgttg ctggaagact ttgagaagtg
     1081 cgatttagtc atttgcattg ggcataaccc tggtacaaac caccctcgca tgctgacttc
     1141 gttgcgcgct ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctc tacaggaacg
     1201 tggcctggag cgatttaccg caccgcaaaa cccgtttgaa atgctgacga actctgagac
     1261 tcagttggcc agtgcctact acaacgtgcg cattggtggc gatatggcgt tgctcaaggg
     1321 gatgatgcgc ctgttaattg agcgcgatga tgctgcaagt gccgcaggtc ggccctcatt
     1381 gcttgatgac gaatttattc aaacgcatac cgtcggcttt gacgagctac gccgtgacgt
     1441 tctcaattcc gagtggaaag atatcgaacg tatttctgga ctaagtcaga cacaaatcgc
     1501 cgaactggct gacgcatatg ccgctgccga acgcaccatt atctgttacg gaatggggat
     1561 cactcagcac gaacatggta cccagaacgt gcagcaactg gtcaatttgc tgttgatgaa
     1621 aggtaacatt ggcaagcctg gtgcgggtat ctgcccacta cgtggtcact ctaatgtaca
     1681 gggcgaccga accgtcggta tcaccgagaa accgtctgca gagtttctgg ctcgcctggg
     1741 tgagcgctat ggcttccccc cacctcatgc acctggacat gctgcaattg ccagcatgca
     1801 agcaatatgt acggggcagg ctcgagcatt gatctgcatg gggggcaact ttgcactggc
     1861 aatgccagat cgggaagcga gcgctgtacc gttaacgcaa ttagatttgg cggtacacgt
     1921 agccactaag cttaaccgct ctcatctgct gaccgcacgg catagctata ttctgccggt
     1981 tctggggcgt agcgagattg atatgcaaaa aagcggtgcg caggcggtaa ccgttgagga
     2041 ttcaatgtcg atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg taatgctgaa
     2101 atcagagtgt gcagtggtcg cgggaatcgc gcaggcagca ctaccccaga gcgtggtagc
     2161 ctgggagtat ctggtggaag attatgatcg cattcgcaat gacattgaag ctgtgctgcc
     2221 agagttcgcc gactataacc agcgcatccg tcatcccggt ggttttcacc tgataaatgc
     2281 agctgctgaa aggcgctgga tgacgccgtc aggtaaggct aatttcatta ccagcaaagg
     2341 gctgttagaa gatccctctt cagcgtttaa cagtaagctg gtcatggcga cagtacgcag
     2401 ccacgatcag tacaacacga cgatttatgg tatggatgat cgctatcgag gggtattcgg
     2461 tcaacgagat gtggtcttta tgagtgctaa acaagctaaa atttgccgtg taaaaaacgg
     2521 cgaaagagtt aatcttattg cgcttacgcc agacggtaag cgcagctcac gccgcatgga
     2581 tagattaaaa gtggtcattt accctatggc tgaccgctca ctggtgacct attttccaga
     2641 atcgaatcac atgctaacac ttgataacca cgatccatta agtggcattc ctggctataa
     2701 aagtattccg gttgaattag aaccatcaaa ttaatgcctc atctcatttc ttctgctgtc
     2761 atccgcacag cagaagaatt cctcattgac tattattgcg caatttgctc acatggatta
     2821 aattaaacta catactataa gatataaact tctgcctaca gctgtaagaa actccgctca
     2881 gtactgaagc accagtccta tttcttcttt tctccagcct gttatattaa gcatactgat
     2941 taacgacttt taacgttatc cgctaaataa acatatttga aatgcatgcg accacagtga
     3001 aaaacaaaat cacgcaaaga gacaactata aagaaatcat gtctgcaatt gtggttgtct
     3061 tattactgac acttacgttg atagccattt tttcggcaat tgatcagctg agtatttcag
     3121 aaatgggtcg cattgcaaga gatcttacac atttcattat caatagtttg caagactgaa
     3181 aataaacagc aaattataaa tgtgaaatgt ttaaaaggta tcgataaaac tttattgttt
     3241 taaggagata aaatgtcgct cgtttgttct gttatattta ttcatcatgc tttcaacgct
     3301 aacattttag ataaagatta cgccttctct gacggcgaga tcctgatggt agataacgct
     3361 gttcgtacac attttgaacc ttatgagcgg cattttaaag aaatcggatt taatgaaaat
     3421 accattaaaa aatatctaca atgcactaac atccagacag tgacggtgcc tgttcctgcg
     3481 aagtttttac gtgcttcaaa tgtaccgact ggattgctta atgaaatgat tgcttatctc
     3541 aactcggaag aacgcaatca tcataatttt tcagaacttt tgcttttttc ttgcctgtct
     3601 atttttgccg catgcaaagg tttcattaca ctattaacta acggtgtgct atccgtttcc
     3661 gggaaagtga gaaatattgt caacatgaag ctggcgcacc catggaagct gaaagatatt
     3721 tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa aactaaagca agagcaaacg
     3781 acattctcac agattctatt agatgccaga atgcagcacg caaaaaattt gatacgcgta
     3841 gaaggttcag tcaataaaat tgccgaacaa tgtggttatg ccagtacatc ttattttatt
     3901 tatgcgttcc gcaaacattt cggcaacagt ccgaagagag tttctaagga gtaccgttgt
     3961 caaaggcaca cgggtatgaa tacgggcaac acgatgagtg ctttagctat ttgattattt
     4021 gctaacgagt agtcaaccac gcacgctacg taagaattat atggggcagc cattccctgc
     4081 cccgcgttgt ttttaggcga tatatttatt gaaataaata agtgacatcc atcacatatt
     4141 tatgcacttg cataacctgt tgcatgatta tttatgatct caattcggca ttttgtcagt
     4201 aaaatgcaat aatttattaa atatcaataa attagttgtt tatcggcgag aaattactta
     4261 atagaacaga aagtaatgtc aacgctttat ggactgttat ttcccttttt ttagctaaat
     4321 ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt cgtaaaatta
     4381 cttcggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg
     4441 tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg
     4501 cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gactttacgc
     4561 cgactgaata cagtaccaaa ggaaagccaa atattatcgt actaaccatg gatgatcttg
     4621 gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg gaaaatcgtg
     4681 aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgca caaaaatcaa
     4741 cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc tatgtggcac
     4801 acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg tcgagctccc gcccgctttg
     4861 gtgtctattc caataccgat gctcaggatg gtattccgct aacagaaact ttcttgcctg
     4921 aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac ttgtcaaaaa
     4981 tcagtaatgt gccggtaccg gaagataaac aaacgcgtga ctatcatgac aacttcacca
     5041 cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt atgggattcc
     5101 acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt gaacgtgtcc
     5161 ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt gttgatcgtg
     5221 ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg cacctaccaa
     5281 atgataatcc tgcaccggat caatatcaga agcaatttaa taccggtagt caaacagcag
     5341 ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt ctcgaacaac
     5401 tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat aatggtgcgg
     5461 ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt cagacctatc
     5521 ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa cccggtaatt
     5581 atgacaagct gatttccgca atggatttct acccaacagc tcttgatgca gccgatatca
     5641 gcattccaaa agaccttaag ctggatggtg tttccttgct gccctggttg caagataaga
     5701 aacaaggcga gccacataaa aatctgacct ggataacctc ttattctcac tggtttgatg
     5761 aggaaaatat tccattctgg gataattacc acaaatttgt tcgccatcag tcagacgatt
     5821 acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg agaaataacg
     5881 attattcgct tgtctataca gtagaaaaca atcagttagg tctctacaaa ctgacggatc
     5941 tacagcaaaa agataacctt gccgccgcca atccgcaggt cgttaaagag atgcaaggcg
     6001 tggtaagaga gtttatcgac agcagccagc caccgcttag cgaggtaaat caggagaagt
     6061 ttaacaatat caagaaagca ctaagcgaag cgaaataact aaaccttcat gcggcggatt
     6121 tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagccc tccagttttc
     6181 aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag tttactcatg
     6241 aaaaatggat ggatgacagc actctgaaag agttcatcaa acaatatatc gaagcgtctg
     6301 gcaatcaggt ctattttacc tggcaaggcg gtgaacccac tctggctggc ctggattttt
     6361 tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgtatt tttaatgcat
     6421 tacaaacgaa tggcatttta ttgaataatg aatggtgtgc ctttctcaaa gaacatgaat
     6481 ttctggttgg tatctcgatc gatggccccc aggagttaca tgaccgttac agacgcagta
     6541 attcaggtaa cggtactttt gcaaaagtga tagcagccat cgagcgtctg aaatcatatc
     6601 aagtagagtt taatacgtta accgtcatta ataacgttaa tgtccattac cctcttgagg
     6661 tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa ttgctagaaa
     6721 ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt atcattgatt
     6781 tttctgtgcc tcccacggct tatggcaagt ttatgtcaac catttttatg caatgggtta
     6841 aaaacgatgt gggtgaaatt ttcattcgtc agtttgaaag ctttgtcagc cgttttttgg
     6901 ggaatgggca tacaagttgt attttccagg agtcctgcaa ggataatctg gttgttgaaa
     6961 gtaatggaga catttacgaa tgcgaccatt ttgtctatcc acagtacaaa attggaaaca
     7021 ttaataaatc tgaactcaaa acgatgaaca gtgtacaact gacagcgcaa aaaaaacgga
     7081 tttcagcgaa atgtcagcaa tgtgcatata aacctatctg caatggcggt tgtcctaagc
     7141 atcgtattac taaagtaaac aatgagactg tttcttattt ttgcgaaggt tataaaatcc
     7201 ttttttcaac catggtacct tatatgaacg ccatggttga gttagctaag aacagagtac
     7261 cgctttacca cattatggat gttgcaaaac aaatggagaa taattaataa cgctctcttt
     7321 taactccatt cttagcctcc ttcttatgac cttaacaagg cacccgacat aaattcctgt
     7381 cgggtgtttc gtcatcatca tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca
     7441 gggcattagc aatacccttg attatcaata gcataaccat aaccataacc actgaaaatt
     7501 aaaaatttct atatatttca tattgttaaa tgatacttaa ataaatttca tcagtaaatc
     7561 attgccatag ataatcattg gatggcacaa tcaattttcg ctaatgataa ccattcccat
     7621 tacgttttgt atgttgatag caaagtatct ctgtctgctg aagccctttt ggctgcgtaa
     7681 gaataataaa acttcagtgt tgttaatcat tattatcctc gcaatgatcc ttggcgtggt
     7741 taaaattcag gtctggttaa acgactggaa taatgatttt ttcaatgcgc taagccagaa
     7801 agaaaccgat aagctttggc agcttgttct ttggttccct gccctgctgg ggatctttgt
     7861 attaatatcc gtaaacaaaa catggttaat taaactactg actattcgct ggcgcgaatg
     7921 gttaacagat tactacctta atcgctggtt tgcagataaa aactactact tcacgcagat
     7981 ctacggcgag cataaaaata cagataaccc tgaccaacgt atcgctgaag acattctctt
     8041 actgatcagc aaaacgctca gtctttcatt tggcttcatc cagtcactta gcatgctgat
     8101 cacctttacc gttattcttt ggcaaagcgc gggtacgctc tcattcactg ttggtggaac
     8161 agaatggaat atccaggggt atatggtcta taccgtcgtg ctcattgtga tcggtggaac
     8221 tttatttacc cataaggtgg gtaaacgtat tcgtccgctt aatgtggaaa aacaacgtag
     8281 cgaagcgaca tttcgaacta atcttgtgca gcataataag caggcagaac tgattgcgct
     8341 aagcaatgct gagtctctac aacgccagga gttgcgcgac aattttcata ccattaaaga
     8401 gaactggcat cgtttaatga atcgtcagcg gtggcttgat tactggcaaa atatttattc
     8461 gcgctcgctt agcgttcttc cctactttct gttattgccg cagtttatta gcgggcagat
     8521 caatctgggc ggactgatga aatcgcgcca ggcatttatg ctagtatcga acaatttaag
     8581 ctggtttatt tataaatatg acgaacttgc tgaactggct gcggttatcg atcgcttgta
     8641 tgagttccat caactcactg aacagcgccc tacgaataag cctaaaaatt gccaacatgc
     8701 ggtacaagtg gctaatgcga gtattcgtac gcctgataat aagatcatat tagagaactt
     8761 gaactttcat gtttcgccag gcaaatggct attactgaaa ggctactctg gtgcgggaaa
     8821 aaccacactg cttaaaacat tatcccactg ctggccgtgg tttaaaggtg atatttcttc
     8881 tcctgctgac agttggtatg tgtcacaaac accgttaatc aaaagcggct tactgaaaga
     8941 gattatttgt aaagcacttc ccctgcccgt agacgataaa tcgttgagcg aagtactgca
     9001 tcaggttggt ctggggaaat tggctgcgcg tattcatgac cacgatcgct ggggagatat
     9061 tctttccagc ggcgaaaaac aacgtatcgc cctggcacga ttaattttac gacgtccgaa
     9121 atggatattt cttgacgaaa ctacctctca tcttgaggaa caagaggcta tacgcttact
     9181 gcgtttagtg cgtgaaaaac tacccacaag cggcgtcatt atggttacac atcaacccgg
     9241 tatctggaac ctggctgatg atatttgtga cattagcacg gttttataac aacaataaaa
     9301 gccctgctgt acggagatat aaataaatga agcgagttct tattcctggc gtcattttat
     9361 gtggcgctga tgtggcgcag gccgtcgatg acaaaaacat gtacatgcat ttttttgaag
     9421 agatgacggt ctatgctcct gtccctgtac ccgtaaacgg caacacgcat tacaccagtg
     9481 aaagcatcga gcgtttaccg accgggaatg gcaatatcag cgatctgctg agaaccaacc
     9541 ctgcggtacg catggattca acgcaaagta cctcgttgaa ccagggagat attcgccctg
     9601 agaaaatctc tattcacggt gcgtcgccct accagaatgc ctatttgatt gacggtatta
     9661 gtgcaactaa taacctgaac ccagcgaatg attccgatgc cagtagtgca accaatatta
     9721 gcgggatgtc acaggggtat tatcttgatg tcagcttact ggacaatgtg acgctttatg
     9781 acagttttgt gccggttgaa tttggtcgct tcaatggcgg ggtaattgat gcaaagatca
     9841 aacgcttcaa cgctgatgat agcaaggtga aattgggtta tcgcactacg cgtttggact
     9901 ggttaacatc gcatatcgat gagaataaca agagcgcatt taatcaaggt tcttcaggaa
     9961 gtacctattt ctctccagat tttaaaaaga acttttatac cttgtcgttt aatcaggagc
    10021 tggctgataa ctttggcgtt accgccggtt tatcgcgccg ccagtctgat atcacccgcg
    10081 cggattatgt ttcgaatgac ggcattgtcg ccggtcgggc acagtataaa aacgttatcg
    10141 atactgcatt gagcaaattt acctggtttg ccagcgaccg ctttacccac gatttaacct
    10201 taaaatatac cggctccagc cgtgattata ataccagcac cttcccgcag tctgatcgcg
    10261 aaatgggtaa taaatcctat ggtctggcat gggatatgga tacgcaactc gcatgggcca
    10321 aactacgtac caccgttggt tgggatcata ttagtgatta tacccgtcac gatcatgaca
    10381 tctggtacac cgaactttca tgtacatatg gtgatattac agggcgttgt acccgtggcg
    10441 gattaggaca catttcccag gctgtagata attacacctt caaaacacgc ctggactggc
    10501 aaaaattcgc cgtgggtgat gtttcgcatc aaccctactt cggcgcggaa tacatctatt
    10561 ccgatgcatg gactgaacgc cataaccagt ctgaatccta tgtgattaat gctgcaggaa
    10621 agaaaactaa ccataccatt taccataaag gtaaaggcag cctgggaatt gacaactaca
    10681 cgctgtatat ggcggatcac attagctggc ggaatgtgtc gttaatgccc ggtgtgcgtt
    10741 atgactatga caactatctg tcaaaccaca atatctctcc gcgctttatg acggaatggg
    10801 atatttttgc tgatcaaacc tcaatgatta ccgccggtta taaccgttac tatggcggga
    10861 atattcttga tatgggatta cgtgatatcc gcaatagctg gacggaatcg gtatcaggta
    10921 ataaaaccct gacgcgttat cagaatttga aaacgcctta taacgatgaa ctggcaatgg
    10981 gattgcagca aaaaatcgat aagaacgtta ttgcacgcgc aaactatgtt taccgtgaag
    11041 cgcatgatca aatcagcaaa agcagtcgta ccgacagcgc gactaaaacc accattactg
    11101 aatataacaa cgatggcaaa accaaaacgc attcgtttaa cctcagtttt gaactggccg
    11161 aacccctgca tatccgccag gtagatatta acccgcaaat tgtctttagc tatatcaaga
    11221 gcaagggcaa cttgtcgtta aacaatggtt atgaggagag caataccggt gataaccagg
    11281 tggtttataa cggtaatctg gtctcttacg atagcgttcc agtggcagat tttaataacc
    11341 cattaaagat ctccttaaac atggatttca cgcatcaacc gagcgggtta gtgtgggcga
    11401 atacgctggc ctggcaagaa gcgcgtaaag ctcgcattat ccttggtaag acaaatgcgc
    11461 aatacatcag cgaatattca gattacaagc agtatgttga cgaaaaactg gatagcagcc
    11521 tgacctggga cacccgcttg tcctggacgc cacaatttct gaaacaacaa aacctgacga
    11581 tcagtgccga tattctcaat gtactggata gcaaaaccgc tgttgataca acgaacaccg
    11641 gtgtggcgac ctacgccagt ggccgtactt tctggcttga tgtcagcatg aaattttaat
    11701 agttcggatt ttacttaacc gggtaaattc gcccggtttt cgcatggaga taattatgag
    11761 aaacctctgt ttcttactga cgttagtggc aactctgttg ctccccgggc ggctgattgc
    11821 cgccgcctta ccgcaggatg aaaagttaat taccgggcaa ctggacaatg gcttgcgata
    11881 tatgatttat ccgcatgctc atccaaagga tcaggtaaat ttatggctgc aaattcatac
    11941 cggttcattg caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat
    12001 gtttaacggc acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg
    12061 cctgcgtttt ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt
    12121 gagtttgccg actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg
    12181 gagtaatgcc gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga
    12241 ggaatggcgt gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct
    12301 gctggctaat acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac
    12361 ggtcacaccg gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac
    12421 ctttatcgtg gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt
    12481 aagtaagctt ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa
    12541 ccacctgcgc tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta
    12601 ttatcgcctg ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg
    12661 gagcatgtta gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt
    12721 gaagactatt tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct
    12781 gtttttccgt gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc
    12841 agagttggca accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc
    12901 tacccgcctc acctggctga aaaatgcggc tgatcagcaa gctgaacgtg atttacgtat
    12961 gctgaccagt cgcctagcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga
    13021 gacatatcaa ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa
    13081 atggcagcag ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct
    13141 tgccgccaaa aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa
    13201 caaaaagctg gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga
    13261 cccacaggcg gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact
    13321 ttccaatggt gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat
    13381 tattgccgta tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc
    13441 gctggcaaat aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa
    13501 acgctggagt gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt
    13561 gctctccgtt agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg
    13621 aatcacccac agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca
    13681 ggcgttgaaa acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac
    13741 gcgctatgct gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc
    13801 cgcagatgcg ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt
    13861 cattgtcggt aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc
    13921 aatcaaacac tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa
    13981 cgcatcggtt actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg
    14041 ttatgattcc cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt
    14101 cgcactggca aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt
    14161 ttcttctcgc ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac
    14221 ttgtcaacca gaacgacatg atgaactgtt aacgttagcg aatgaagtga tggttaagcg
    14281 tttgactaag gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag
    14341 cctcgatatc caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat
    14401 tcaatatgac gatcctgcag catggactga gcaggagcaa ttgttgaaac aaatgacggt
    14461 agagaatgtt aacactgccg ttaaacaata tctttctcat ccggtaaata cttataccgg
    14521 agtattattg ccaaaataat aacagccccg tcaacacatc gttggcgggg attttagcaa
    14581 tattcgctat ttttatgtaa taattttata aatgcgttca aaataataac caagtactaa
    14641 tagtgatatt ttaatgtctg atttttacgt gataattcag gaggcacaga aagcgcataa
    14701 aaataacaac ataaaacacc ttgccaccac ccaagaattc tctattgcat tgttttttaa
    14761 tgaaaaaata tcattccaac aatatctcac gataaataac attaggattt tgttatttaa
    14821 acacgagtcc tttgcacttg cttactttat cgataaatcc tactttttta atgcgatcca
    14881 atcattttaa ggagtttaaa atggataaga agcaagtaac ggatttaagg tcggaactac
    14941 tcgattcacg ttttggtgcg aagtctattt ccactatcgc agaatcaaaa cgttttccgc
    15001 tgcacgaaat gcgcgacgat gtcgcatttc agattatcaa tgatgaatta tatcttgatg
    15061 gcaacgctcg tcagaacctg gccactttct gccagacctg ggacgacgaa aacgtccaca
    15121 agttgatgga tttatccatt aacaaaaact ggatcgacaa agaagaatat ccgcaatccg
    15181 cagccatcga cctgcgttgc gtaaacatgg ttgccgatct gtggcatgcg cctgcgccga
    15241 aaaatggtca ggccgttggc accaacacca ttggttcttc cgaggcctgt atgctcggcg
    15301 ggatggcgat gaaatggcgt tggcgcaagc gtatggaagc tgcaggcaaa ccaacggata
    15361 aaccaaacct ggtgtgcggt ccggtg
//
LOCUS       DADLSB010000095        15227 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.095, whole genome shotgun sequence.
ACCESSION   DADLSB010000095 DADLSB010000000
VERSION     DADLSB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.095"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            214..1515
                     /gene="rstB"
                     /locus_tag="JN331_002732"
     CDS             214..1515
                     /gene="rstB"
                     /locus_tag="JN331_002732"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY7260845.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            1591..2520
                     /gene="tus"
                     /locus_tag="JN331_002733"
     CDS             1591..2520
                     /gene="tus"
                     /locus_tag="JN331_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY7260846.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2517..3920)
                     /gene="fumC"
                     /locus_tag="JN331_002734"
     CDS             complement(2517..3920)
                     /gene="fumC"
                     /locus_tag="JN331_002734"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY7260847.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(4063..5709)
                     /locus_tag="JN331_002735"
     CDS             complement(4063..5709)
                     /locus_tag="JN331_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY7260848.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5908..7083
                     /gene="manA"
                     /locus_tag="JN331_002736"
     CDS             5908..7083
                     /gene="manA"
                     /locus_tag="JN331_002736"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY7260849.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7184..8692
                     /locus_tag="JN331_002737"
     CDS             7184..8692
                     /locus_tag="JN331_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7260850.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8918..10183)
                     /gene="uidC"
                     /locus_tag="JN331_002738"
                     /pseudo
     CDS             complement(8918..10183)
                     /gene="uidC"
                     /locus_tag="JN331_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(10222..10389)
                     /locus_tag="JN331_002739"
     CDS             complement(10222..10389)
                     /locus_tag="JN331_002739"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HAY7260851.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(10405..11401)
                     /gene="uidB"
                     /locus_tag="JN331_002740"
                     /pseudo
     CDS             complement(10405..11401)
                     /gene="uidB"
                     /locus_tag="JN331_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11398..13209)
                     /gene="uidA"
                     /locus_tag="JN331_002741"
     CDS             complement(11398..13209)
                     /gene="uidA"
                     /locus_tag="JN331_002741"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY7260852.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLDIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13597..14187)
                     /locus_tag="JN331_002742"
     CDS             complement(13597..14187)
                     /locus_tag="JN331_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7260853.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14416..15183)
                     /gene="hdhA"
                     /locus_tag="JN331_002743"
     CDS             complement(14416..15183)
                     /gene="hdhA"
                     /locus_tag="JN331_002743"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY7260854.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         3841 a         3939 c         3603 g         3844 t
ORIGIN      
        1 agactaaaga acaacaaagc gtcacgttaa attaaacctt agcataataa acaagccccg
       61 gtggaaaatt gaatttactg aatatgaata cgccggggat acatccgtct gttccggaca
      121 ttatcttcca aacaaggtct tatccagttg gcggtttaca ttgctccaca gtcgtgaatt
      181 aatacgaata gtattctgtt gcgggcgatg ccaatgaaaa aattgtttat ccagttttac
      241 ctgttattgt ttgtctgctt ccttgtgatg tctctgctgg ttgggctggt gtacaaattt
      301 accgccgaac gcgcgggcaa acagtcgctg gatgatttga tgaacagttc gctgtatctg
      361 atgcgcagcg aattgcgaga gatcccccca cacgactggg gtaagacgct gaaagagatg
      421 gatttaaatc tctctttcga tctgcgtgtc gagccactga gtaaatacca tcttgatgat
      481 atttccatgc accgactgcg tggcggcgaa attgtcgccc tggacgatca gtacacgttt
      541 ttgcagcgta tcccgcgcag ccactacgtg ctggcagttg gtcctgttcc ttatctttat
      601 tacctccatc aaatgcgatt gctggatatc gccctgatcg cttttattgc tatttccctc
      661 gcctttccgg tgtttatctg gatgcgtccg cactggcagg atatgttaaa actggaagca
      721 gcggcgcaac gatttggcga tgggcatctc aatgaacgta tccactttga tgagggttcg
      781 agctttgaac gacttggtat cgcatttaac cagatggcgg acaatatcaa cgccttaatt
      841 gccagcaaaa aacagcttat tgacggtatc gctcacgaac tgcgaacacc gttagtgcgc
      901 ctgcgttatc gactggagat gagcgataac ctgagcgccg ccgaatccca ggcgttgaat
      961 cgtgatatca gtcaacttga agctttaatt gaagagctgc tgacttatgc ccgactcgat
     1021 cgcccacaaa acgagcttca tcttagcgaa ccagacctgc cgttgtggct gtcaacgcat
     1081 ctggcagata ttcaggcagt aacgcccgat aaaacggtac ggataaaaac gctcatgcaa
     1141 ggccattatg cggcgttgga tatgcgctta atggagcgcg tgctggataa tttgctcaat
     1201 aacgccctgc gctactgcca ttcaacggtt gaaaccagcc tgctactgtc ggggaataga
     1261 gcgacattaa ttgttgagga tgatggccca gggattgccc cagaaaaccg cgaacatatc
     1321 tttgaacctt ttgttcgcct cgatcccagc cgggatcgct caaccggcgg ctgcgggctg
     1381 gggctggcaa ttgtccactc tatagcactg gcaatgggcg gtacggttaa ttgtgacacc
     1441 agcgaactgg gtggtgctcg cttctcgttt agctggccgt tatggcataa catcccgcaa
     1501 tttacctctg cctgacacta cgcgcacgat ggtcaagtca ccacgactgt gctataaaat
     1561 aagtatgttg taactaaagt ggttaatatt atggcgcgtt acgatctcgt agaccgactc
     1621 aacactacct ttcgccagat ggaacaagag ctggctacat ttgccgctca tcttgagcaa
     1681 cacaagctat tggttgcccg cgtgttctct ttgccggagg taaaaaaaga ggatgagcat
     1741 aatccgctta atcgtattga ggtaaaacaa catctcggca acgacgcgca gtcgctggcg
     1801 ttgcgtcatt tccgccattt atttattcaa caacagtccg aaaatcgcag cagcaaggcc
     1861 gctgtccgtc tgcctggcgt gttgtgttac caggtcgata acctttcgca agcagcgttg
     1921 gtcagtcata ttcagcacat caataaactc aagaccacgt tcgagcatat cgtcacggtt
     1981 gaatcagaac tccccaccgc ggcacgtttt gaatgggtgc atcgtcattt gccggggctg
     2041 atcaccctta atgcttaccg cacgctcacc gttctgcacg accccgccac tttacgcttt
     2101 ggttgggcta ataaacatat cattaagaat ttgcatcgtg atgaagtcct ggcacagctg
     2161 gaaaaaagcc tgaaatcacc acgcagtgtc gcaccgtgga cgcgcgagga gtggcaaaga
     2221 aaactggagc gagagtatca ggatatcgct gccctgccac agaacgcgaa gttaaaaatc
     2281 aaacgtccag tgaaggtgca gccgattgcc cgcgtctggt acaaaggaga tcaaaaacaa
     2341 gtccaacacg cctgccctac accactgatt gcactgatta atcgggataa tggcgcgggc
     2401 gtgccggacg ttggtgagtt gttaaattac gatgccgaca atgtgcagca ccgttataaa
     2461 cctcaggcgc agccgcttcg tttgatcatt ccacggctgc acctgtatgt tgcagattaa
     2521 cgcccggctt tcatactgcc gaccatctgt tctggccgta cccagctgtc aaactcggct
     2581 tcgctaagat accccagcgc aagggccgca gcttttaagg tcagcccttc tttatgcgct
     2641 tttttggcga tctccgcggc tttgtcataa ccaatgtggg tgttaagcgc agtcaccagc
     2701 atcagcgatt cattgagtaa ttgattgatt cgctcacgat tcggttcaat acccactgcg
     2761 cagtgtttgt taaaactttc catgccatct gccagcaagc gcaccgattg caggaaatta
     2821 tggatcacca tcggacggaa gacgttcagt tcaaagttac cggaagcgcc acccatgttg
     2881 atcgccacgt cgttccccat tacctgacag cagagcatgg ttaatgcttc acactgggtt
     2941 gggttcactt tccccggcat gattgagctg cccggctcat tttccgggat tgagatttca
     3001 ccaattccgc agcgcgggcc agaggccagc cagcggacat cattggcgat tttcatcagt
     3061 gacgcagcca accctttcaa cgcgccgtgc gcctgaacca gggcatcaca ggtcgcaagc
     3121 gcttcaaatt tgttcggcgc ggtaacaaac ggtgcacagg taatgactgc cagttcatct
     3181 gctacgcgac gcgcatactc cggatgggta tttagtccag tacccaccgc tgtaccgcca
     3241 agagccagtt ccgctacgtg aggcaggctg tattcgatat gtttgagatt atgctcgagc
     3301 atcgctaccc agccggaaat ctcctgccct agtgttagcg gcgtggcgtc ctgcaagtgg
     3361 gttcgaccga ttttgacgat atcggcaaat gcacgcgatt tttcactcag cgtctgtgtc
     3421 agggttttaa gctgcggaat gagttgcttg cgcagtgcca gcagcgccgc aacgtgcatc
     3481 gccgtcggaa agacatcgtt ggaactttgg cttttgttca cgtcgtcgtt agggtgaact
     3541 ttacgttcca tcccgcgcac accgccgagt aattcactgg cccggttagc cagcacttcg
     3601 ttcatgttca tgttactttg cgtgccggag ccggtctgcc agatagccag cgggaattcg
     3661 tcgtcatgct gtcctgccag tacttcatcc gccgcctgcc gaatggcgct cgctttctct
     3721 tcagacaaca agtctaaatc ttcattaact tttgccgctg cgcgcttggt tagcgccagc
     3781 gcatgaatca gtgatgtggg cattttctcc gtcgaaatgc ggaaatgctc cagcgagcgt
     3841 tgagtttgtg cgccccacag cttatctgcc gggacatcaa tcgcccccat cgaatctttt
     3901 tcgctgcgta ctgtattcat ggcctgctcc tcacctgatt aattttttct ttctgttttg
     3961 ctttcgttaa gcaactttta gctcactcat tatttactat ttgataacaa atgtttggtc
     4021 tttcgtgcca tgtaaaaaaa ccgccccgaa gggcggctct gtttatttca cacagcgagt
     4081 gcactgtgtg agttgtatct gctggaagaa gtcatttcct ttatcatcca caaggataaa
     4141 cgccgggaaa tcttccactt caattttcca gatggcttcc attcccagtt ccggatattc
     4201 aacacattcc aggctcttaa tacttccctg cgccaataca gcggccggac caccgatact
     4261 gccaaggtag aagccgccgt gttttttaca ggcatccgtc acctgctggc tgcggttgcc
     4321 tttcgccagc atgatcatac ttccgccctg cgcttgcagt tgatcgacat aagaatccat
     4381 ccgtccggcg gtcgttgggc caagagaacc ggaggcataa ccttccggcg ttttggccgg
     4441 acccgcgtag taaatcggat gatctttaat gtattgcggc agcccttcac cgttatccat
     4501 ccgctctttc agtttggcgt gagcaatatc acgaccaacg ataatcgtgc cgttaagcga
     4561 taagcgtgta gaaacgggat actgcgacaa ctgtgcgagg atctctttca tcggacggtt
     4621 aaggtcaacg cgcaccgctt cgccttctcc cgctttacgc agctcttcag ggatatattt
     4681 acctggatta tgttccagtt tttcgatcca gatcccctga cggttgatct tcgctttgat
     4741 attacggtca gcagagcagg aaacacccat accaaccgga caggatgcgc cgtgacgtgg
     4801 caggcgaatc acgcggatgt cgtgagcgaa gtatttacca ccaaactgcg cacccagacc
     4861 gagattttgc gcttcgatca gtaattcttt ttccagttcc acatcgcgga acgcctgacc
     4921 gtgctcattc ccttccgttg gcagttcatc atagtatttc gcggaagcca gtttcaccgt
     4981 tttaaggttc gtttctgcag aagttccacc aataacgaac gcaatatgat acggaggaca
     5041 ggccgcggta cccagcgtgc gcatcttctc aaccaggtaa tttttcagtt tccccggcgt
     5101 cagtaacgct ttggtttcct gatagagata cgtcttgttt gccgaaccac caccttttgc
     5161 gatacagagg aatttgtatt cgtcgccatc aacggcataa agatcgatct gcgctggcag
     5221 attggtgccg gtattcactt ctttatacat atccagcggc gcgttttgcg agtagcgcag
     5281 attatcttcg atataagtgt tatagacacc gcgcgccagc gccgcttcat caccaccacc
     5341 ggtccataca cgctgccctt ttttaccaac aataatcgcg gtgccggtat cctgacaggt
     5401 tggcagaacg cctttcgccg cgatgtcgga gttacgcagg aattgcagcg ccacatattt
     5461 atcattttcg ctggcctccg ggtcacgcag aatgtcggcc acctgttgtt ggtgagccgg
     5521 acgcagcata aacgacgcat catgaaacgc ctgacgcgcc aacagagtta acgcttcggg
     5581 tgcgactttc aaaatctcct gcccttcaaa ttcagatacg ctaacgtgtt cactggttag
     5641 caggtaatac tcagtatcat cttttttgag tggaaaagga gactgataat gaaagggttt
     5701 gtttgacatt gttctctcac ttactgcctg gtttggttat gctctgggcg ggtgttccgt
     5761 tgccctgtta aaagcgagta acaatatcct acacagtttt ttaacaaaaa ctgagactag
     5821 tacgactttt tgcggctcca ggttacttcc cgtaggattc ttgctttaat agcgggatta
     5881 atttccacat taaaacaggg attgatcatg caaaaactca ttaactcagt gcaaaactat
     5941 gcctggggta gcaaaacggc gttgactgaa ctttatggta tggaaaatcc gtccagccag
     6001 ccgatggccg agctgtggat gggcgcacat ccgaaaagca gttcacgagt gcagaatgcc
     6061 gccggagata tcgtttcact gcgtgatgtg attgagagtg ataaatcgac tctgctcgga
     6121 gaggccgttg ccaaacgctt tggcgaactg cctttcctgt tcaaagtatt atgcgcagca
     6181 cagccactct ccattcaggt tcatccaaac aaacacaatt ctgaaatcgg ttttgccaaa
     6241 gaaaatgccg caggtatccc gatggatgcc gccgagcgta actataaaga tcctaaccac
     6301 aagccggagc tggtttttgc gctgacgcct ttccttgcga tgaacgcgtt tcgtgaattt
     6361 tccgagattg tctccctact ccagccggtc gccggtgcac atccggcgat tgctcacttt
     6421 ttacaacagc cacatgccga acgtttaagc gaactgttcg ccaacctgtt gaatatgcag
     6481 ggtgaagaaa aatcccgcgc gctggcgatt ttaaaatcgg cgctcgacag tcagcagggt
     6541 gaaccgtggc aaacgattcg tttaatttct gaattttacc cggaagacag cggcctgttc
     6601 tccccgctgt tactgaatgt ggtgaaattg aatcctggcg aagcgatgtt cctgttcgct
     6661 gaaacaccgc acgcttacct gcaaggcgtg gcgctggaag tgatggcgaa ctccgataac
     6721 gtgctgcgtg caggtctgac gcctaaatac attgatattc cggaactggt tgccaatgtg
     6781 aaattcgaag ccaaaccggc taaccagttg ttgacccagc cggtgaaaca aggtgcagaa
     6841 ctggacttcc cgattcctgt ggatgatttt gccttctcgc tgcatgacct tagtgataaa
     6901 gaaaccacca tcagccagca gagtgccgcc attttgttct gcgtcgaagg cgatgcaacg
     6961 ttgtggaaag gttctcagca gttacagctt aaaccgggtg aatcagcgtt tattgccgcc
     7021 aacgaatcac cggtgactgt caaaggccac ggccgtttag cgcgtgttta caacaagctg
     7081 taagagctta ctgaaaaaat taacatctct tgctaagctt attaaaggct tataacacct
     7141 tcaggcggcc agtccgcctg atttcatttt atggataatc attatgaata aatcgctggt
     7201 agcggtaggc gtcattgttg cgctaggcgt agtctggaca ggcggcgcat ggtatacagg
     7261 caagaagatt gaaacccatc tcgaagacat ggtcgcgcag gcgaacgcgc aactcaaact
     7321 gacagctcct gaatccaacc tggaagtgag ttatcaaaac tatcatcgcg gcgtattcag
     7381 cagccagttg caactgttgg tgaaacccat tgccgggaaa gaaaatccgt ggattaaaag
     7441 cggtcagagc gtcatcttca acgaatcggt tgatcatggt cccttcccgc ttgcccagct
     7501 taaaaaactg aacctgatcc cgtcgatggc atcaattcaa accacgctgg ttaataacga
     7561 agtaagcaaa ccactgtttg atatggcaaa aggtgaaacg ccttttgaga ttaactcgcg
     7621 cattggttac agcggtgatt ccagttccga tatttcgctc aatccactga attacgagca
     7681 aaaggatgaa aaagtcgcct ttagcggcgg cgagttccag ttaaatgctg acagagacgg
     7741 caaagccatc tccctttccg gggaggcgca aagtggtcgg atagacgcag ttaacgaata
     7801 caaccagaaa gtgcagttga cctttaataa tctgaaaacc gacggttcca gcacgctggc
     7861 aagttttggt gagcgcgtag gaaaccaaaa actgtcactg gaaaaaatga ccatttcagt
     7921 ggaaggcaaa gaactggcac tgctggaagg catggagatc agcggtaaat cggatctggt
     7981 caatgacggt aaaacgatca atagccaact ggattactcg ctaaacagcc tgaaggtaca
     8041 gaatcaggat ctgggcagcg gcaagctgac tttaaaagtc ggccagattg atggtgaagc
     8101 ctggcatcag tttagccagc aatataacgc gcaaactcag gcgctgctgg cacagccaga
     8161 aattgccaac aatcccgaac tttatcagga gaaagtgacg gaagccttct ttagcgccct
     8221 gccgctgatg ttgaaaggcg atccggtgat tactatcgcg ccgctaagct ggaaaaacag
     8281 tcagggtgaa agtgcgctga atctgtcgct gttcctgaaa gatccggcaa cgactaaaga
     8341 agcgccgcaa acgctggcgc aggaagtaga tcgttcggtt aaatctctgg atgcgaaact
     8401 gaccattccg gtggatatgg caactgagtt gatgactcag gtagcgaagc tggaaggtta
     8461 tcaggaagat caagcgaaaa aactggcgaa acagcaagtt gaaggtgcat cagcaatggg
     8521 gcagatgttc cgtctgacca ccttgcagga caataccatc accaccagcc tgcaatatgc
     8581 taacggtcag ataacgttaa atgggcagaa aatgtcgtta gaagattttg ttggcatgtt
     8641 tgcgatgcca gctcttaacg ttccggctgt accggcaatt ccgcagcagt aattcacggc
     8701 caaaacgccg gagaattttc actccggcgt tttcatctga aaacaaacca gagattgcct
     8761 gatgcgctac gcttatcagg cctacctttt cctgcaacac tttgaattta tgagtttttg
     8821 taggctggat aaagcgttta cgccgcatcc ggcataaaaa acgcgcactt tgtcaacaat
     8881 ctgaaacgcc ggaggttttt ctctccggcg ttatttctta cttcagcata aagtcatact
     8941 gaatactcat tttcacatcc cagtaattca ggcgatcgcc cagcgtactg ccatcgcgct
     9001 tttcccgccc tttatcggca gcaagaaaga aattcatccc ttgtaacggc ccttcgctga
     9061 cccgatagtt cataccacct aagatacccg cgccttcaat ccgcacatct cgtttactgg
     9121 attcatagcc gcgagtcaca atcggtgcaa atgtcagcgt aaattgcgga gttacacggt
     9181 agttgacacc taattgccag gactgcattc cttcacggtt acgatcaaga cttaatgaaa
     9241 agggatagcc aatgtcggta tccaccacgg caccattcat tgcatggcgc aaataaccac
     9301 cactgccaaa tacgcttccc cacggcgcat tctaggtcaa ttgtccacta accaacgccg
     9361 tttcattggg ctggctggta ttacgactta aaccttcgag ctgcgcgtaa aagccgagca
     9421 actccccttt caacgtataa ttcgggtcga gcggtatgct tttctcgaga atactttgta
     9481 tctgattgga aacatctttc tgtaccgatg cccccagaga atagcgactg gtatcatcat
     9541 gccaactggc ggctaaaaaa gaacgtgcgc gatgtttatc gtattttccc gtctggctgt
     9601 tatacacccc ttcatcacga ataccggttg ttgaatcagt ccataaaaat actcgtgaac
     9661 gccacattcc ccacagttca atatttggcg ttaaagccca ataacctaat gctccttcag
     9721 aaactgtcgg taatgtacgt tgcgaactgc cagccattaa cggaatacga taaggtaagc
     9781 taccataact ataatccgtc ccgaagcgac ctaatttaag caggctattt tcactgggtt
     9841 ttatttttac tgcccccagg gcaaagccaa aacttttatc accatcaaga taccaccggg
     9901 tactcatatc agcacgagca cctaatttat aaacataata cgctccgcct tcaacgccaa
     9961 taatattgga ataataacca ctattgaaat cgagcaatcc tccctgtacc catgcgtaaa
    10021 tatctcttcc cgcgccagca ctgggcttat cagccctgcg caattcgttt ttcagtctga
    10081 tgcgtagtcc cgcttcatcg tcagccagtt gcgccgcgta agcagaggta aggccagagg
    10141 cagccgtcag gcaaataacg gccatggcca ctatttttct catagttgca cctttgatgt
    10201 tctgattatt tttattgaat attaattagt gatatcgctg attaattgct gctgcacttt
    10261 tttacgatta tcaatttcaa ccacgatttc tttgaatttt ttatccgtga gcggataaaa
    10321 ccagataata acgaatgcca gtagcataaa tccgcaaggt actaaggcaa ttgatgtgcg
    10381 gatgcccata ataacttccg gcgtttacac ctgattggcg atatatccgc ttaatcccaa
    10441 aataaaggca ggaattgaac ctccgattgc ctgaccgcat ttacgggtaa atgagaatag
    10501 tgaataggtg agcccttcaa ttcgcacgcc ggtcaggtat tcaccgtatt ctacggtatc
    10561 agcttccagt gcccacatca cagtcatagt aacgccctga ccaagtgaac agcccggtat
    10621 catttaacac atagcgcacg tagaacaacg acgaggcgct gaccgcgaag gtcgaaatca
    10681 gcacacacag cgcaccgatg cacaacataa acagcgggcg attccgtttc agggtttgca
    10741 aactgatctt caatgacggc tgcgccacga tacgtaccac attttcacgc gtcgatttga
    10801 agcagatgaa gtaaagcacc attccggcaa tcgccagcac aatcgtccag aaatggtata
    10861 ccgacaccat ctcttccggg ctggagttct taatgctcgg tcctatcaga aatgccaggc
    10921 atacaaaggt caatgaagcg gcaatcccac gagccgcgcc cagacgggcg cgggattgtg
    10981 gttgttgggt catcgcggta gcaagtgaac cataaggaat attcaccagg ctgtagcaaa
    11041 gcccgaggcc catgtaggtc aaatatgcat acaccacttt gctaccatgg ctccagtcgg
    11101 tcggtaccca gaataccagc acgctgaaga tcattaacgg cgcagtaccg aagagtaaaa
    11161 acgggcggaa ttttccccag cgggtattca cactgtccac cactcgtccg gcaaagacgt
    11221 cggcgaaggc atcgaatacc cgcaccagta acagcatggt gcctgccgca gcggcaccga
    11281 cgccagcgac gtcggtgtag taactcaata ggaagagcgc ccccattgcg aaggcgaagt
    11341 tattggcgac gtcaccgagg ctgtagccga cgatggtgcg ccaggagagt tgttgattca
    11401 ttgtttgcct ccctgctgcg gtttttcacc gaagttcatg ccagtccagc gtttttgcag
    11461 cagaaaagcc gccgacttcg gtttgcggtc gcgagtgaag atccctttct tgttaccgcc
    11521 aacgcgcaat atgccttgcg aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac
    11581 gacggcgctg acgcgatcaa agacgcggtg atacatatcc agccatgcac actgatactc
    11641 ttcactccac atgtcggtgt acattgagtg caggccggct aacgtatcca cgccgtattc
    11701 ggtgatgata atcggctgat gcagtttctc ctgccaggcc agcagttctt tttccagtac
    11761 cttctctgcc gtttccaaat cgccgctttg gacataccaa ccgtaataac ggttcaggca
    11821 cagcacatca aagagatcgc tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac
    11881 gcaggtgatc ggacgcgtcg ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata
    11941 ttcccgtgca ccttgcggac gggtatccgg ttcgttggca atactccaca tcaccacgct
    12001 tgggtggttt ttgtcacgcg ctatcagctc tttaatcgcc tgtaagtgcg cctgctgagt
    12061 ttccccgttg acagcctctt cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc
    12121 aatgtctaaa gagaggttaa agccgacagc tgcagtttca tcaatcacca cgatgccatg
    12181 ttcatctgcc cagtcgagca tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt
    12241 ggccccaatc cagtccatta atgcgtgatc gtgcaccatc agcacgttat cgaatccttt
    12301 gcctcgcaaa tccgcatctt catgacgacc aaagccagta aagtagaacg gtttgtggtt
    12361 aatcaggaac tgttcgccct tcactgccac tgaccggatg ccgacgcgca gcgggtagat
    12421 atcacactct gtctggcttt tggctgtgac gcacagttca tagagataac cttcacccgg
    12481 ttgccagagg tgcggattca ccacttgcaa agtcccgctg gtgccttgtc cagttgcaac
    12541 cacctgttga tccgcatcac gcagttcaac gctgatatca ccattggcca ccacctgcca
    12601 gtcaacagac gcgtggttac agtcttgcgc gacatgcgtc accacggtga tatcgtccac
    12661 ccaggtgttc ggcgtggtgt agagcattac gctgcgatgg atcccggcat agttaaagaa
    12721 atcatggaag taagactgct ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg
    12781 gatagtctgc cagttcagtt cgttgttcac acaaacggtg atacgtacac ttttcccggc
    12841 aataacatac ggcgtgacat cggcttcaaa tggcgtatag ccgccctgat gctccatcac
    12901 ttcctgatta ttgacccaca ctttgccgta atgagtgacc gcatcgaaac gcagcacgat
    12961 acgctggcct gcccaacctt tcggtataaa gacttcgcgc tgataccaga cgttgcccac
    13021 ataattacga atatctgcat cggcgaactg atcgttaaaa ctgcctggca cagcaattgc
    13081 ccggctttct tgtaacgcgc tttcccacca acgctgatca attccacagt tttcgcgatc
    13141 cagactgaat gcccacaggc cgtcgagttt tttgatttca cgggttgggg tttctacagg
    13201 acgtaacata agggactcct cattaagata aaaatactgg tcaaccttta atctgattag
    13261 ttaaccaatt ctgtgttgca cacagttatc tgtgagaaac ccatggaaga gaaatggaaa
    13321 taaaaagaac tgacaccgcg atcaaaaaac caaaaactgc aatacccaca gcgtgatatg
    13381 gatcgcatta atctttaaaa cagcgttgac agaagccata aaataagcaa ccgaacgttc
    13441 gttcgagagt attgggaacg ataaactctc atgacatatg gctacggcga atattttggg
    13501 ggggaattct ccgaacacgg ggaggataat ttttgcacag agaaattacg atatgaaacg
    13561 cgggcacagt tatcaccagc ccgcgaaatg agaggatcag gatgcggtta atataccgcc
    13621 aatcatcgcg cgtaatccct gagcaaacga cgcttcacgc gccaggtctt ccgcttcgat
    13681 atcggccagt acaccgtagg tcagatccag cagtaaacgt gaagtcattg ccgtgttgat
    13741 gtctgggctt atctcgcctt tttgctgggc atcacgcatc cgctgggcaa caaattcagt
    13801 gatcgtcata tgcttatttt taagcatggc ggcgacctgt gggttacgca tcccttccgc
    13861 cataatttcg accaccagcg cccgttgccc aaaggcttca tgggtgagag agacaataga
    13921 ctcgaccata tagtcaacaa aatgaatccc ttcaatcggt tcccggaaac gagccagcgc
    13981 cctctcctgg tcctgtaaga taatcgcctg aatcaaggct tctttggaga tgaaatggtg
    14041 atagagcgtc ccaggactaa tggcgcaaga tttacagatg gctttcatcg aggcactgtg
    14101 aaatccattt tctgaaaaaa tctctctggc agcattgagg atccgggtcc gtgtcggttg
    14161 tgcttcagtc tgcatgttat ccatcattaa accattatag ttagcgtgaa taactagttt
    14221 accgcgttgt caggtgagcg cctatcattg ctgttgaata gtgcgaaggt acactctatt
    14281 cgtatacttt tcacccagca attggttacc caatagccgt gatttggctg ccagttgttt
    14341 agcagagacg ttgatgaatt gcacacgcta tcccggtaaa caacacatac gaccgtttac
    14401 cggttcgtta gtgtattaat tgagctcctg taccccacca ccggagacgg tgagaatttg
    14461 tccgcttacc cagctggctg caggcgagca aaggaacagc gccgcgttag caatatcttg
    14521 cggttggccc agacgtctga tcggcgtgtg ctgtaacatt ttttgttcaa tttctggtgt
    14581 aataacggat ttcagggcat cggttaatat tgcccccggc gcaatgccat tcacccgaat
    14641 atttttttca cccaggtcaa atgccatatt tctgaccaga tgactggccg cagctttaga
    14701 tgatgcatag gaagtcatgt ttatattttt attttctgcc gccatagaag tgatggtcag
    14761 aataacgcca ccgccatttt tttccatttc tggcgcaaca agttgtgaca gatggaaaaa
    14821 agaaaacaca ttcagttcat aagcacggcg aaaatctgcc attggcatat caaacggttt
    14881 aggaccaccg ccaccggcgt tgttaaccag gatatcaact ttacccagtt tactgacggc
    14941 aaagtctgcc agtgcagaga gttcctgttc ggaagtaata tcacaacgcc aggcaaatgc
    15001 ctgaccaccc agttgttgaa tttcgtctac aacatggtta gctgcgtcgg cgttaatatc
    15061 actgaccacc acagatgcgc cagctgtcgc gaatgtaatg gcgatttctt taccaatacc
    15121 tgcacccgca cctgtgatga tggcgcattt tccgtcgagt ctcaggttgt cagaattaaa
    15181 cacataaacc tcctgtgaac ttcagtaaaa ataaaaggca ccgcctg
//
LOCUS       DADLSB010000096        15128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.096, whole genome shotgun sequence.
ACCESSION   DADLSB010000096 DADLSB010000000
VERSION     DADLSB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.096"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(48..782)
                     /gene="araH"
                     /locus_tag="JN331_002744"
                     /pseudo
     CDS             complement(48..782)
                     /gene="araH"
                     /locus_tag="JN331_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
     gene            complement(794..2308)
                     /locus_tag="JN331_002745"
     CDS             complement(794..2308)
                     /locus_tag="JN331_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAY7260855.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2378..3367)
                     /locus_tag="JN331_002746"
     CDS             complement(2378..3367)
                     /locus_tag="JN331_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7260856.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            4164..4667
                     /locus_tag="JN331_002747"
     CDS             4164..4667
                     /locus_tag="JN331_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY7260857.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4747..4998)
                     /locus_tag="JN331_002748"
     CDS             complement(4747..4998)
                     /locus_tag="JN331_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY7260858.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(5113..5199)
                     /gene="azuC"
                     /locus_tag="JN331_002749"
     CDS             complement(5113..5199)
                     /gene="azuC"
                     /locus_tag="JN331_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY7260859.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5462..5785
                     /locus_tag="JN331_002750"
     CDS             5462..5785
                     /locus_tag="JN331_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260860.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5956..6453
                     /gene="ftnA"
                     /locus_tag="JN331_002751"
     CDS             5956..6453
                     /gene="ftnA"
                     /locus_tag="JN331_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY7260861.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6490..6729)
                     /locus_tag="JN331_002752"
     CDS             complement(6490..6729)
                     /locus_tag="JN331_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY7260862.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            6919..8130
                     /gene="tyrP"
                     /locus_tag="JN331_002753"
     CDS             6919..8130
                     /gene="tyrP"
                     /locus_tag="JN331_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY7260863.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLATAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(8192..8857)
                     /gene="yecA"
                     /locus_tag="JN331_002754"
     CDS             complement(8192..8857)
                     /gene="yecA"
                     /locus_tag="JN331_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY7260864.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(9053..9139)
                     /locus_tag="JN331_002755"
     tRNA            complement(9053..9139)
                     /locus_tag="JN331_002755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9103..9105),aa:Leu,seq:taa)
     gene            complement(9152..9225)
                     /locus_tag="JN331_002756"
     tRNA            complement(9152..9225)
                     /locus_tag="JN331_002756"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9191..9193),aa:Cys,seq:gca)
     gene            complement(9280..9355)
                     /locus_tag="JN331_002757"
     tRNA            complement(9280..9355)
                     /locus_tag="JN331_002757"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9320..9322),aa:Gly,seq:gcc)
     gene            complement(9507..10055)
                     /gene="pgsA"
                     /locus_tag="JN331_002758"
     CDS             complement(9507..10055)
                     /gene="pgsA"
                     /locus_tag="JN331_002758"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY7260865.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(10112..11944)
                     /gene="uvrC"
                     /locus_tag="JN331_002759"
     CDS             complement(10112..11944)
                     /gene="uvrC"
                     /locus_tag="JN331_002759"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY7260866.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11941..12597)
                     /gene="uvrY"
                     /locus_tag="JN331_002760"
     CDS             complement(11941..12597)
                     /gene="uvrY"
                     /locus_tag="JN331_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY7260867.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            12893..13069
                     /locus_tag="JN331_002761"
     CDS             12893..13069
                     /locus_tag="JN331_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260868.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            13056..13280
                     /locus_tag="JN331_002762"
     CDS             13056..13280
                     /locus_tag="JN331_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY7260869.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(13313..13933)
                     /gene="sdiA"
                     /locus_tag="JN331_002763"
                     /pseudo
     CDS             complement(13313..13933)
                     /gene="sdiA"
                     /locus_tag="JN331_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(14163..14915)
                     /gene="yecC"
                     /locus_tag="JN331_002764"
     CDS             complement(14163..14915)
                     /gene="yecC"
                     /locus_tag="JN331_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY7260870.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(14912..>15128)
                     /locus_tag="JN331_002765"
     CDS             complement(14912..>15128)
                     /locus_tag="JN331_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7260871.1"
                     /translation="SFISLVKDTSLAATIQVPELFRQAQLITSRTLEVFTMYLAASLI
                     YWIMATVLSTLQNHFENQLNRQEREPK"
BASE COUNT         3840 a         3874 c         3457 g         3957 t
ORIGIN      
        1 gcaaacatgg actggcaaaa gtgtagcggt attaagcgca gctatttagt catacgtgaa
       61 gccagaataa ttccggctat cgcagatacc aggccagaga gaacaaagat aataattttg
      121 gtgcgaacaa ccggtacacc cgccagacgc gcagcctctt cgttcccgcc aatcgccagg
      181 gtattacgac caaaggtggt tttattcagc agcaaaccaa agataatcag acacgcgacg
      241 gtgagccaga ttggcgcagg cagaccgaac cagttagcgt aaccaagggc aaagaagctt
      301 tcatcttcga taccgaccgc tttaccgtct gaaatgatgt acgccagacc tcgaacaatc
      361 tgcatcgttg ccagtgtcgt gatcagagca tttattttca gtttggcgat aacaaagcca
      421 ttgaccaggc cacagagaac gcccagcgac aaacccgctg ccacgccaat ccacaggctt
      481 tcagtcaggt tgataaccac cgccgtggtg acacccgcac aggcaattac ggaggcgaca
      541 gaaaggtcaa agtcaccgga agcaaggcag aacaacatgc cacacgccac catccccgac
      601 atggaaattg ccaggcccaa ccctttcata ttaatgaagg tggcaaaatt tgggacaaaa
      661 atggcacagg cgataaagag caccgcaaac accaccagca tgccgtactg atcccagata
      721 cgcccgaagc tgaatgacga cttaggtgcg ccagaccccg atgtagaaac agaagacatc
      781 atactctcct tactcaggca actgcctggc tgactttagg catcgcaagg ctcagtgcct
      841 gacgctcatc tgcctgctcg tgtaacaatt caccggcgat ttcgccttcc cgcatcacca
      901 caatccggtc ggcaacgccg aggacttcag gtaagtcgct ggaggcaaac agcaccgcca
      961 cgccctgcgc cgccagcgca taaattacgt tatatatttc gtgcttagcg ccaacatcaa
     1021 tgccgcgcgt aggttcatcc agcaaaatga ccttcatctc ttccgataac cagcggccca
     1081 gaatggcttt ttgctgattt ccgcctgaga gattcatgat cagttgctcc gcgcccggcg
     1141 ttttgatgtt gagcgaacga atgtgctgat cggcattgtt ttcttcccaa ctgttgttga
     1201 ttacacaacc gccgagcaca tgtttgcgtc tggcactgat gttgatattg tcgcgaacgg
     1261 agtgcacggg aataatgcct tccgctttgc gagcttccgg gcagagcatc atgcctgcgg
     1321 caatggcgtg gctcggttta cgaatatcga tcggctgttg gtcgatataa acctgaccgg
     1381 cggtgatttg cgtcccgcca aacaagcctt tcattaattc gctacgcccc gcccctacca
     1441 gaccaaacag cccaacaatt tcaccactgc gaaccgccag acttattggc gtacgcacgc
     1501 ctggtgcttt cacagcatca agacgcaggc gttcctcgcc ataactacga ggttgccagc
     1561 cgtagatatc gccaatgtcg cgcccgacca tcgcctgcac cagcgcgtcg tggtcaacct
     1621 gctgcatatc ggtaaaggtt ttgacataac gtccatcttt aaagacagtg atggcatcgc
     1681 tgagggcaaa tatttcttcc atacggtgag aaacgtataa gatcacccgc ccctcttttc
     1741 gcagttcacg aataacgcgg aaaagattgc cgatttcgcg ggcagaaagg gagctggttg
     1801 gctcatcaaa ggcgataatt ttagcgttac gcgccagcgc tttggcgatt tcaaccatct
     1861 gccactgacc aatggagaga tatttcagcg gcgtgtccgg gtcaatatcc ataccaagat
     1921 gtttaagttg taaacccgcc tcataattca gcaatgagcg attcacaatg ccgcctttat
     1981 gcggcagctg gccgagatag atgttttccg cgacggtcat ttccggcacg agatgcagtt
     2041 cctggtaaat aatcgccaca cccgcgttaa gtgctgcggt cgtgtcggaa aaggacattt
     2101 cctgcccatt aatcactaca gaacccgtgg ttggcgcata gttgccgctg aggattttta
     2161 agagagttga ttttcctgcg ccattttcac ccatcaacgc atgaacctga ccggcatagc
     2221 agtcaaaact aatatccgtc agcgccttaa cgccgggaaa cgttttaccg atgccgcgaa
     2281 atgagagata cggggtagac tgttgcataa cgtctccgtg aatcactggt cgttcacacc
     2341 ccctctgcat catgcagagg ggaatttttc cggcaaatta cttaccgcct aaaccttttt
     2401 tctccagttc ttctttaaag ttgtcacgcg tgatcagtac cacgtctgta acttcggtaa
     2461 attttggcgt ttcaacgtct tttgctaccc agttgtaaag catttcgctg gatttatagc
     2521 catgtacgtc cgggcttggc agcagggaac cgtagaagcc ggttgcctgt gctttagaca
     2581 gttcgcttac tgcatccaca ccgttaatac caataccgat gatatcggct gctttaaagc
     2641 cctgaccttc ggttgcacgt acgccgccaa gtacggtgct gtcgttcata ccgacgatca
     2701 gccaatgttt aacttccgga tgttgaacca gcattgagtt ggcagcgtca aatgcccccg
     2761 ggatgtcgtt agatttggta ggtacctgat aaatttgttt ttccgggaat cctgcagctt
     2821 tcagtgcgtc catagaaccc gttgtacggc ggcgggcggt atccagttcg ttggcggtaa
     2881 tcgccatcac tgcgctttct ttgacatccc agccacgttt ctgcatctct ttatacagtt
     2941 cctggccctg acgttcgcca attttagtcg ccgccatcat caccagagga acggtatcca
     3001 ttggcttacc tttggcgtta acaaactggt catccactgc aatgactttc atatcgtagc
     3061 cacgcgcttt cgcgacgatg gcagagccga gtttcgggtc cggagtacaa ataacgaaac
     3121 cttttgcgcc actggctgcc aggctgtcga tcgcgttcaa tgttttttcg ccatccggca
     3181 cggcaatctt aataacctca aaccctaaat ccttcccggc tttatcggca aacttccatt
     3241 cggtctggaa ccacggctct tccggttgct tcaccagaaa accgagcttc aggttctccg
     3301 ccatagcgga ttgtgacata acggctgcca gaccaatggc tgccagggct ttagtaaatt
     3361 tgtgcatggt tctctccagc tttagtgtcg ttttgtgtag ggcaaaaacg aatgacattc
     3421 gttaaattaa tcggaaaaca aagcattacc ttttatctaa aagataagtg accgtgttga
     3481 catagtttta gcgagaaatt aattctccat aggagagcaa tatcacatcg cagaattaca
     3541 gtgagaacgt gcataaattt agcgggaaaa gacataagga aaagccaatt tgtcagacaa
     3601 attgttgaat gcacagcaga ttaatccata agattagcct ggaaatcctt gttgtctttg
     3661 gtacccatgc gggatgtctt ctttttaacc agtcaatagg ccgcattacc tggcgttgag
     3721 tttttgaaat ggtgtaataa ccacaactca aagatgtgga aaatgcgcgt cattcatttc
     3781 gccattaatt atcactgtgc tcattaatta acagaacacg tataatgaga gccatctcgc
     3841 aaaaatgaaa aaacgtttta taaaatcatc acttcatcat gaattcaaat tcatcgatta
     3901 atatcaacaa gatacaaaaa gcactatcat taaaattcat tgcatttaca ttgatttcac
     3961 caatgaaatg taaaaatata taaacttgat gatttaagca ttttcttata accgttcaga
     4021 cgttattctt atttcagatc atcgtcagaa ttgactccac gatcacattt cggaccggca
     4081 gaaaggaatt attctgcaaa cagtaattat ggtgttttga tttatcttgc acctctccac
     4141 ttctggatat aaggatatta ggtatggcaa ccgctggaat gcttctcaaa ctcaactctc
     4201 aaatgaaccg cgagttttac gcatccaatc tctaccttca cctgagtaac tggtgttctg
     4261 aacagagtct gaacggcacc gccactttcc ttcgcgccca ggcacagagt aatgtgaccc
     4321 aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc tacccccatc gttaaagcca
     4381 ttgatgttcc aggtgaaaaa ctgaactctc tggaagaact gttccaaaaa acgatggaag
     4441 aatacgagca acgttcaagc acgctggcgc agttagccga tgaagcgaaa gaactgaatg
     4501 atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga acagcagcat gatggcctgt
     4561 tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact tgcgggtatg tgccctgtgc
     4621 agaccgacca acatgttctg aatgtcgtgt cacaccagct gcattgatca tacatcggcg
     4681 ctaatacatt gcgccgatga agtttttgag aaaccgctgc ctcatctgtt tgaagcagcg
     4741 gtttttttaa tgggattcac cctgtggggt aaacttgagt tcaatcagcg cgatggcttt
     4801 ttggattgcc cgcatggtga ccgggtctgc ggcagcggga tgatgagtaa agtcgatatt
     4861 cttcagctga ctggacattt tttcacgaac ttcaacgggc gcgattacat cgagaacatc
     4921 cagaatttgt ttgataacca actggcaagc taccacatca gaaaccagtt cctgatcggc
     4981 attcagcggc tgggacatcg taaactcctg atagcatttt gaaagccgtt atagtagcgg
     5041 cttcacatct tcagcgatag tcacatccac cgtcatcagg acacaaaaaa acctgccgga
     5101 gcaggttttt tgttatcgga acatattgcc tggcggtacg tctttgaacg tcttgcaata
     5161 gttattgaac atacttttca ggattttgcg cagtttcatc gcggcactcc gaccatttgt
     5221 tatacaggag ttattgtctt tgcgcttata atatgacaac catcacaaaa atcaatcttt
     5281 atgtgataca aatcacataa ataccccttt aatgttataa aaatgataat caaaaaacag
     5341 cccccctatt tctgacacct acagatggca agaaatagcg cctgccaggc gtcttttccg
     5401 gccattgtcg cagcgctgta acgcgtaaaa tagtgctttc tcttactctt ctggctggac
     5461 catgagactt ctgattctga ctctttcatt aataacgctg gcggggtgta cggttactcg
     5521 ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt gtacggttgg tttatgacca
     5581 ggcttttttg cagcatgccc ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc
     5641 atgccagcag gaaggctata cccacgcgat cccctttggt cagccagtag gcaactgtag
     5701 cctttttgcg ggttccttat gtttgaatac ggaattcact ttgtcatatc agtgccatca
     5761 ttccgccttc cctgtctttc tctaaatatt ttaatcagcg aggggatctt cgctgattaa
     5821 agaaatagcg gtaatgcgtt ttattcccat tcgtattttt aataattgaa gtttatattt
     5881 taccttttgc aaataataaa ataacaaatt atagtgacgc cacagcaaca aatataacct
     5941 ttgtggagca ctatcatgct gaaaccagaa atgattgaaa aacttaatga gcagatgaac
     6001 ctggaactgt actcttcact gctttatcag caaatgagcg cctggtgcag ctatcatacc
     6061 ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg aagagatgac gcatatgcag
     6121 cgtctgtttg attacctgac tgataccggc aatttaccgc gtattaatac cgttgaatct
     6181 ccgtttgctg aatattcctc acttgatgaa ttattccagg aaacctataa acacgaacaa
     6241 ttaatcaccc agaaaattaa cgaactggct catgctgcaa tgaccaatca ggactaccca
     6301 acatttaatt tcctgcagtg gtatgtttct gagcagcatg aagaagagaa actgttcaaa
     6361 tcgattattg ataaattaag cctggcaggc aaaagcggcg aaggtctgta ttttatcgac
     6421 aaagaactct ctaccctcga cacacaaaac taatgctctc cggcggcagg tttctgccgc
     6481 cgtttcaaat cagtgacggc aaatcttact ctgatcggtc gaaaaacctt cttctgaagg
     6541 tataaatttg ccttttgctg ccagaaacgc taccagctct cccgctgtca tcccttctgc
     6601 cgagcacgtg tgaaaacgtg cctgttcacc aaaacgcgct ttaatcgcag cttccagact
     6661 ggcatgcgta tattgctcgc ctgattcaat catcatattt aacacttcat gaccgtgaat
     6721 agagtccatc gttcctcctc aaaaaaagcc tagcgtagcg attacctctt atgaagactt
     6781 tgcgccagcg caggactgaa tgcttttatt gtacatttat atttacacaa tatgtaacgt
     6841 cggtttgacg aagcagccgt tatgccttaa cctgcgccgc agatatcact cataaagatc
     6901 gtcaggacag aagaaagcgt gaaaaacaga accctgggaa gtgtttttat cgtggcggga
     6961 accacaattg gcgcaggcat gctggcaatg ccgctggcta cggccggtgt tggttttagc
     7021 gttacgttaa tcttgttgat tgggctttgg gcgttgatgt gctacacggc gctattactg
     7081 ctggaggtgt accagcatgt tccggcagat accggtctgg gcacgctggc aaaacgctat
     7141 ctgggacgct acggtcaatg gctgacgggc ttcagtatga tgttcttaat gtatgctctg
     7201 actgcggcat acatcagcgg tgccggtgaa ttgttggcct ccagcatcag cgactggaca
     7261 ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca cttttgttgc cggtggcgtg
     7321 gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt ttctgttcag cgccaaaatt
     7381 atttttctgg tggtgatgct ggtattgctg ctaccgcata ttcacaaagt gaatctttta
     7441 accctgccgt tgcaacaggg gctggcactg tctgcaatcc cggtgatttt tacgtcgttt
     7501 ggttttcacg gtagcgtgcc gagtattgtc agctatatgg atggcaacat tcgtaagcta
     7561 cgctgggtgt ttataatcgg tagtgccatc cccctggtgg catatatttt ctggcaggtg
     7621 gcgacgcttg gcagcattga ttcaacaacc tttatgggat tgctggctaa tcatgctgga
     7681 ttaaacgggc tgttacaggc gttacgcgaa atggtggcct ctccgcatgt tgagctggcg
     7741 gtacatttat ttgctgattt agccctcgcc acgtcatttc tcggcgttgc gttaggctta
     7801 tttgattatc tggctgattt gtttcagcgt tcaaataccg ttggtggacg attgcaaact
     7861 ggtgcaatta cctttctgcc gccgttggcg tttgcactgt tttatccacg aggatttgtg
     7921 atggcgctgg gttacgccgg tgtggcgctg gcggtactgg cattgattat cccttcacta
     7981 ttgacctggc aaagcagaaa gcacaatcct caggcgggtt accgggtcaa aggtggtcgt
     8041 ccggcgctgg tggtggtgtt tctctgtggt attgctgtga ttggcgtgca atttttgatt
     8101 gcggcagggt tgttaccaga agtggggtga tcagatagcc tcaaattcct tattgggtgc
     8161 cagaattaac gctgacaccc aatgtgacct cttaatgcag gcagcactgc ttaaatttct
     8221 taccactgcc gcacggacaa ggatcgttac gccccggttt ctcttctgct ttgatcggtt
     8281 gctgaacagc tttttcctgc ggatgcgcca tccagtatgc atgtaaatca agcgccgcca
     8341 gtcgaatggc atctacgctc tcttcaaacg cttctggcga cattttctct acccgctcga
     8401 agttttcctc agtaccgtgc agcgcaatcg cctccagcgc tggttttaaa gaatcgggca
     8461 acgttgacca gtcagaaagt gccacgcccc gcatatagcc aaagcaccac tcctcaacaa
     8521 tcgtcagctc gctgccatca acttctcgca agccgaataa cggctcaaac tgctccggga
     8581 attcgttcag acgctctgcg gtatcggcca tatgttgaaa agccaggttc ataaagcgcg
     8641 tcatctcttt ctctgacgcc cagcgcggca catagtcagc cccaccccac acggcaacca
     8701 gccactgttc tggttcaatc tcttgcggag aactcaacac cgccgtcaat aaaccgtcca
     8761 gctccgccac atcaaggatg gcgtggtcag tgttgtattt ggtcagaata tcgtccagcc
     8821 attccaactc actttcgttt aacggtcccg ttttcatacg cttttccttg tggatctcaa
     8881 ctcgccagca cctatcttac atgccggtcc gtatcagaga tactttttga gtggctttgc
     8941 cggtgattaa aaattaagga gggtgtaacg acaagttgca ggcacaaaaa aaccacccga
     9001 aggtggtttc acgacactgc ttattacttt gattttattc ttatctttcc catggtaccc
     9061 ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc cttgtgtcta
     9121 ccgattccac catccgggct cgggaagaaa gtggaggcgc gttccggagt cgaaccggac
     9181 tagacggatt tgcaatccgc tacataaccg ctttgttaac gcgccaaatt cttcaggcct
     9241 ttcagccaga catccgcttg acgccgatgt cttttaaact ggagcgggaa acgagactcg
     9301 aactcgagac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt cccgcattca
     9361 tcaagcaatc agttaatcac ttgattttat tatcgtctgg caatcagtgc cgccgttcga
     9421 tgcgttgcat tctacttacc tggcgcgatg agtcaacgat atttttcacc acttttgatc
     9481 gtttgctgaa aattacgccg aaacgatcac tgatcaagca aatctgcacg cgcagcgctc
     9541 aaatattgca acattgacca cagagtcagt accgcagcca caaagaaaag tgcaataccg
     9601 gcgtactcaa cccaaatgtt cggacgccac agcagccagg ccaacgccac catctgggca
     9661 gtggttttca ctttcccaat ccaggagacg gccacgctac tgcgtttacc caactccgcc
     9721 atccattcgc gtagcgcaga aataataatt tcacgggcga tcatcgttgc cgccggtaat
     9781 gtcacccacc agctgtgata atgctcggtt accagcacca tggcgatagc cacgagaact
     9841 ttatctgcca cagggtcaag gaaagcacca aaccgggtac tctggttcca gcggcgtgcc
     9901 agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga aaatgagcgc ggcggcaaac
     9961 ggcgaccagg tgacaggcag ataaaagacc aatacaaaga atgggataag gatgacacgg
    10021 aacagtgtaa gcaacgtagg gatattaaat tgcataatga cgggtaacta tctgttgtca
    10081 gtaagattac ccctatgttg ctacagagac atcaatgttt caacgaccag aagatctttt
    10141 ctgccagacc ttgcgaaata cccggcactt ttgcaatttc ctcgacgctg gcgttacgta
    10201 aaccttgcaa accgcccata tatttcaaca acatttgccg acgttttggc ccgacgcctt
    10261 caatggtttc cagggaactg gtatttttga ccttcgcccg ttttttacgg tgcccgccaa
    10321 tcgcgtgatc atgtgattca tcgcgaatat gctggataac atgcagcgcg ggtgaatctg
    10381 gcggcaaact aaatccctca ccttccggct caaagaacag cgtttccagt ccagccttac
    10441 gatctgctcc tttggcaacg ccaagtagca gcggatgatt tttatcccat gagacatcca
    10501 gttcggcgaa gacatttttc gcctgcgcaa gctggccttt gccgccgtcg ataaggatca
    10561 catccgggat cttactgtcg tcaatggctt taccataacg ccgacgcagc acctgattca
    10621 tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt atagcgccga tactccgcac
    10681 gcagcgggcc gttagcatca aacaccacac aggaagcgac ggtttgttcg cccatggtat
    10741 ggctgatgtc aaagcactcc atccgcttca cttccggcaa tttcaacacg ctggcaagcg
    10801 cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt gctggttaag gccgtcgccg
    10861 cattggtgcg cgcgagtttc agataacgcg ccctatcgcc gcgaggtttg gtttgaacat
    10921 taatcttgcg tcccgccagt tctgaaaggg aatcggcgag cagcgtttta tcgctaagat
    10981 taaaatcgag caggatctca cccggtaagg tgcgcatctg gctgccttgt aaatagaact
    11041 gacctacgaa ggtttctacc acctcgctca gttccgtacc gccaggcact ttcgggaaat
    11101 agctgcggct gccgagcact ttgccctgac gaatgaacaa tacgtggaca caagccatgc
    11161 ccgcatcgaa cgccacacca ataacgtcaa ggtcgtcgcc ggtattggaa acaaactgtt
    11221 tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat acatgccgct tcttcaaact
    11281 ccagattctg gctggcagtt tccatacggc taatgagttg cgtaagcacc tgatcatctt
    11341 tgccagacaa aaacaggcgc acatactcga cctgctgagc gtattcttct tcactcacca
    11401 gtccttcaac gcacggtccc agacagcgcc ctatctggta ttgcagacac ggacgcgagc
    11461 gattgcgata aacactattt tcgcactggc gaatggggaa aatcttttgc agtagcgcca
    11521 gtgtttcacg tacggcatag ccattcggga acgggccgaa atattcacct ttggcatgct
    11581 ttgcaccacg atgcatcgcc agacgcgggt gggtatcgcc actcaggaag ataaaaggat
    11641 aagatttatc atcgcgtagc aaaacgttgt aacgcggctg atagagtttg atgtagttgt
    11701 gttccagcaa cagcgcttcg gtttctgtat gcgtaaccgt tacatcaatt tgctggatct
    11761 gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct gcggaaatag ctggaaagcc
    11821 gttttttcag gtctttcgct ttgccgacat agataaccgt accaccagca tcgtacatgc
    11881 gataaacgcc tggctggctg gttacggttt ttaaaaacgc ttttgcgtca aactgatcac
    11941 tcactgactt gataatgtct ccgcattaca cagaccatgg cgaattgcca ggtgagtcag
    12001 ctcaacatcg ccatgaatgt ttagtttact gaacatacga tagcggtagc tgttcaccgt
    12061 tttcggactg agattgagct gttctgagat ctcattgacc ttctggccct tagtgatcat
    12121 cagcataatc tgcaattcac gttcagacaa actggcaaat gggctttctg ttttttctgg
    12181 ttcgatctgg cttaacgcca tttgttgagc gatatcagaa gcaatgtaac gctgcccaga
    12241 atagacagaa cgaatcgcac tcacgacttc ctgcggagcc gcgcctttgc tgaggtagcc
    12301 agcagcacct gcctgcatga ctttcgctgg taaagggttt tctgtatgga ccgtaagcat
    12361 gatgatttta acatcagctg tggaacgcgc gattttacgc gtcgcctcaa gaccgccaat
    12421 gcccggcata ctcatgtcca ttagcaccac gtcaacggca tttgtccggc accacttaac
    12481 ggcgtcttca ccgcacgatg cctctccgac gacttttata ccctttatat cttccagaat
    12541 gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca agtagaacgt tgatcaaagg
    12601 aatatctcca gaaataggga taacgctact gatagttagt cattcgtata ttagcggttt
    12661 ttattgcaac tttgaaacgt taaaaatggt gggtattcga tttttctctc gtttttgtca
    12721 attcgattgt ccacagttat ggtcacgccc gtcaccaacg gcttaccgga tcgtttcaca
    12781 gtaagcccta cgatgaaatg tgacaaaaat tgactttatt cagcaaaaat gaaaatcagc
    12841 ccgctgaata tgtaacatta attaacccga acaatacggg caaaaaacat tcatgataaa
    12901 aatatttatt ggtcattata ttaacgtatt ttatagcact gccgatatca cgctcaaaaa
    12961 acaaccactg ctatttttag caaagcttat ggtatactcc gccgccttaa catttttcac
    13021 cgcaaatttt cattgcaaca tgacgaggaa aataaatgag tacgcctgat ttttctactg
    13081 ccgagaataa tcaagaactg gcaaatgaag tctcctgcct gaaagcgatg ctgacgctga
    13141 tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat gttaaagatg gaaaaacagc
    13201 ttgcgctgat cgaagacgaa acccaggctg cagtattttc caaaacggtt aagcaaatta
    13261 aacaagccta ccgtcagtaa tataaaaccg gctgagagcg tgcctttcag ccggttattg
    13321 acaaaatcat cgctatctct gctgatgtct tcccttccgc cgtccatttc agaatttctt
    13381 tttcgcgctt gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca
    13441 gagccatcag actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa
    13501 tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc cgatttggca
    13561 gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg
    13621 gctgtgcttc gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag
    13681 ggttcagcac aggatcaatt gcgagaaaat tttttgcctg ataataacta acccacgact
    13741 cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac
    13801 ataacgaata gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct
    13861 cttctgcggc ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat
    13921 ccgtatcctg catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat
    13981 tcaaactatc acataaataa gatttatata taacttatat tattcaggca atgaattact
    14041 tttgcaatcc atcgcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt
    14101 ttatcacgct tgaaatggcg tcccgccccg acaggtgaat cgtcggggct gattttttct
    14161 tattattgca gcagaagctt ctcgaggaac tggcgggtgc gaggctgctg gggatcggca
    14221 aataacgctt ttgctgcccc ctgctcgact atccgcccct ggtccataaa gatcgcccgg
    14281 tccgcaacat cccgggcaaa gctcatttcg tgtgtcacaa tcaccatcgt gcgcttttcc
    14341 tgcgccagct ggcggatggt gttcaggact tcgcccacca gctctggatc cagcgccgac
    14401 gttggctcgt caaacaaaat cacctcagga cgcattgcca gtgcacgcgc aatcgcaaca
    14461 cgctgctgtt gaccgccaga caaacgacgt ggatagctgg tttctttacc ggccagccca
    14521 actttcgcca gcagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg
    14581 atcaccggcc cttcaataat gttttccagc accgtacgat gcggaaacaa attaaagttc
    14641 tggaagacaa acccgacgtg ctgacgcaac tggcgaatca gcgatttttg ctgacttaat
    14701 gaacgtgcag tatcaatagt gatatcgcca acggtgatcg ttcccgcttc cggttgttcc
    14761 agcagattta tgctacgtag caatgtggtt ttgccggaac cactcggacc gataattgcc
    14821 accacttcgc caggctttac ctcgaggtcg ataccgtgca gcaccgtctg accgtggaat
    14881 tttttcacca ggttcttaac ttcaatggca ctcattttgg ttctctctcc tggcgattaa
    14941 gttgattctc aaaatggttc tgcaacgtcg ataacacagt cgccatgatc cagtagatga
    15001 gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct
    15061 gacggaacag ctccggcacc tggattgtcg cggccagcga cgtatctttt accagactga
    15121 taaagctg
//
LOCUS       DADLSB010000097        15127 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.097, whole genome shotgun sequence.
ACCESSION   DADLSB010000097 DADLSB010000000
VERSION     DADLSB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.097"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..250
                     /locus_tag="JN331_002766"
     CDS             <1..250
                     /locus_tag="JN331_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAY7260872.1"
                     /translation="AGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVHQYGIDAVFS
                     ILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(478..1263)
                     /gene="allE"
                     /locus_tag="JN331_002767"
     CDS             complement(478..1263)
                     /gene="allE"
                     /locus_tag="JN331_002767"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY7260873.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKNGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGTSFVDYLVTLHQNCGNQQCFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(1274..2509)
                     /gene="allC"
                     /locus_tag="JN331_002768"
     CDS             complement(1274..2509)
                     /gene="allC"
                     /locus_tag="JN331_002768"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY7260874.1"
                     /translation="MITHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FADAMKACEFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQPLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(2531..3580)
                     /gene="allD"
                     /locus_tag="JN331_002769"
     CDS             complement(2531..3580)
                     /gene="allD"
                     /locus_tag="JN331_002769"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HAY7260875.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLCLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKVAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            3897..5564
                     /locus_tag="JN331_002770"
                     /pseudo
     CDS             3897..5564
                     /locus_tag="JN331_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
     gene            5574..6833
                     /locus_tag="JN331_002771"
     CDS             5574..6833
                     /locus_tag="JN331_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAY7260876.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            6844..7659
                     /locus_tag="JN331_002772"
     CDS             6844..7659
                     /locus_tag="JN331_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAY7260877.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            7656..8549
                     /locus_tag="JN331_002773"
     CDS             7656..8549
                     /locus_tag="JN331_002773"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY7260878.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(8686..9753)
                     /gene="purK"
                     /locus_tag="JN331_002774"
     CDS             complement(8686..9753)
                     /gene="purK"
                     /locus_tag="JN331_002774"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY7260879.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(9750..10259)
                     /gene="purE"
                     /locus_tag="JN331_002775"
     CDS             complement(9750..10259)
                     /gene="purE"
                     /locus_tag="JN331_002775"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY7260880.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(10377..11099)
                     /gene="lpxH"
                     /locus_tag="JN331_002776"
     CDS             complement(10377..11099)
                     /gene="lpxH"
                     /locus_tag="JN331_002776"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY7260881.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(11102..11596)
                     /gene="ppiB"
                     /locus_tag="JN331_002777"
     CDS             complement(11102..11596)
                     /gene="ppiB"
                     /locus_tag="JN331_002777"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY7260882.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            11770..13155
                     /gene="cysS"
                     /locus_tag="JN331_002778"
     CDS             11770..13155
                     /gene="cysS"
                     /locus_tag="JN331_002778"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY7260883.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(13191..13712)
                     /locus_tag="JN331_002779"
     CDS             complement(13191..13712)
                     /locus_tag="JN331_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7260884.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(13820..14032)
                     /gene="ybcJ"
                     /locus_tag="JN331_002780"
     CDS             complement(13820..14032)
                     /gene="ybcJ"
                     /locus_tag="JN331_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY7260885.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(14034..14900)
                     /gene="folD"
                     /locus_tag="JN331_002781"
     CDS             complement(14034..14900)
                     /gene="folD"
                     /locus_tag="JN331_002781"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY7260886.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAARLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
BASE COUNT         3534 a         3940 c         4146 g         3507 t
ORIGIN      
        1 ggcaggcggt aaagcgccgc tgggcgttgc gtcggtggcg aagcagttta atgtaccggt
       61 gattgggatt gctggtgtat tgggtgatgg cgtggaagtg gtgcaccagt acggcattga
      121 cgcggtattc agcattttgc ctcgtctggc acctttagcc gaagtgctcg ccagcggtga
      181 aaccaatctc ttcaacagcg cgcgaaatat tgcctgcgcc attaaaatag gtcagggaat
      241 taaaaactga ctcttgcctt taaagcggat gcgatttatt tcgtataaga gcgcagtact
      301 catgccggat gcggcatgag tactatatcc ttcctgaaaa tcgcgcaaat tctatatatt
      361 gcagaggtca tgtaggcctg ataagcgaag cgcatcaggc aatgttacaa aaaaagccac
      421 ggtataaacc gtggcaaaat ccaacatagc taaaaataat caggcgagtg gtatgactta
      481 aatctctacg tcgcggttgc aatctttcga gtaaatatag ctgaacgctt caccacgccc
      541 tacgccataa ccagcctgta aagaataagc gcccataaag atgtaatcgc cttttttcac
      601 cgggatccag ttattgtcga ggttataaac cccctgaccg gaaagaatat aggcaccatg
      661 ttcctgaacg tgtgtttcga tataaccgtg gctggcacct ggtgcaaaag agaggatatg
      721 catgttcata tcaaaaccta actctttggg cagaaaatcc agcaggatca catcatccat
      781 accttcatag tgaatgcgtt ccagttcgct ggcattgcca gaaaccagcc acggtgcatg
      841 gccttctacc ggaacatagc ggcgcttata taaaaagatt tggctgtctt cggcctgggc
      901 gttaacaaac gtcattaagg agcctggtgg gcaataaaga tagccacctt cgcttaaggc
      961 aaatgttttg ccttcggctt tggcagtgat atttccagag atcacataca ggaacgtttc
     1021 aatgccttcg ccaccgaagc actgttggtt gccacagttt tgatgcagtg tgaccagata
     1081 atcaacaaaa gaggtaccca gctttggcgt ggagaggatt gtcgcgtcac aattttcaaa
     1141 gcccggaata atatttttta ccagaccatc cggggttaac agtgcgaaat taccgttttt
     1201 aacaatcgca cggttagcca gtaaatcttc gcggtaaccg gtgacgttat ttaaatatcc
     1261 catttatgac tccttatttc tgccaggcaa gttgataaag catgagtgcc aacgttttga
     1321 ccccttcggc aaggtcagta atattggtgc gttccgccgg gttatggctg atcccgttga
     1381 tgctggggat aaaaatcatg caggttggta cgcgaggcgc gaaaatttgc gcgtcgtgcc
     1441 cggcaccact gtgcatcacc cggtaattca gtttttctct ttcacacaat tctgtcaggg
     1501 tggcgaccag ctccttattc atcggcacgg gttcttcgtc catccataaa tcgatatcaa
     1561 taccaatgtc catttcatcg caaatcgccc gcatgtcgtt ttctaacggt tgggtgaaat
     1621 cgcgcagcac ggcagcgtcg gtatgacgac aatcaatggt gaacgtggtt ttacccggca
     1681 ccacatttac cgtattcggg cgcggctcta ctttgccaaa ggtcagaacc agcggatcgc
     1741 ccatcttttt cgctttttcg atcgactgat ggcaaatgcg actgaaagcg taaaccgtat
     1801 cacgacgata acccatcggc gtggtgcctg catggtttga ttcgccgttc agcgtcaccg
     1861 tatagcgacg ctgcccgaca attgcattca ccacgccaat tgattgccca ttactttcca
     1921 gcacacagcc ctgttcaata tgcagttcaa caaaggcttt tatatcctga cgcggagtta
     1981 gtggggcgtt cggcagagta aattcgcaag ccttcatcgc atcggcaaaa ctatttcctt
     2041 tggcatcaca gatattccgc acgtcgtcag gattcgccag cccaaagata tttttactgc
     2101 cccagaagac atacgggaag cggctgcctt cttcttctgc catcgccacc acttcgaccg
     2161 tacgtaacgg tgcgccgtat tgcgttttca accagtcaat tgccagccac gccgccagcg
     2221 cgccgaattg cccgtcgagg ttaccgccgt taaccacggt atcgatatgc gaaccgctca
     2281 gaaccacttc ctgtggatat tcggtgccac tcaggcgacc gtataaattc cccacttcat
     2341 cgaaacgtgt ttccagccca cttgctgcca ttcttttttt aaattgctgc tgggtttcca
     2401 gccattccgg cgaataaagt aaacgggtca tcccacccgt tgggtcagcg ccaaaagagg
     2461 aaagccaggg cagcgtttct tctatagctt gacgggaatg tgtaatcata agaaagtcct
     2521 gtctcaataa ttattgcgca aagggatttt tcgtttcgta tgacgtgtta taaagcgcgt
     2581 cggaaattaa atactggtaa atatcatcaa caatttcgat gccttcgacg gcgactttgc
     2641 gttgtttaat atcctgatcc tgtccgggat aataaacctg attaaaaccg ggcgcggggg
     2701 taatggcatt taattcgcgc atggtctggc taagatgttg acggaataat tcgctggagg
     2761 agaaaaagtt cggattaata actatatgta attgccccaa attacgccct gcgtgtaaat
     2821 cgtcatacat cgaactaacc tgtcgcccga acggtaagca gagtaagacg cctgagagga
     2881 cgtcaatcat catcatcagg ccataccctt ttggcccagc ggcggggagc agagcatgta
     2941 ccgcgaacgg atcggttgtt ggtgcaccgt ttttatcgac cgcccaggta tccgggatag
     3001 acatattacg cgagcgggcg tcgagcactt ttccccatgc ctgtacggta gtcgccatat
     3061 caaaggtaag gatctcgtcg ccttctcccg gtgcggcaaa ggccagtggg ttagtaccgt
     3121 agtaaatttc cgcgccgcca aacggcacca ccattggatc ggactggcac atcgaaatgc
     3181 caattaatcc ggcgcgggct gcctgctgca caaaataaga gattgcgccg ctgtgaccca
     3241 tccggctgat accgaccacc gcaacgcctt tttgctgggc ggttttgatg gcatgttcca
     3301 tacccatttt cgccgcgacc tgcccggcgg cattgtcagc atgtaaaatt gccgagcacg
     3361 gcccggtttc ctcaagacga aactccggtt cgcggttggt gccgcctttt gaaatgcgtt
     3421 ccgcgtagta ttccacgcgc accgcgccat gagagtggat ccctctggca tcggcgtaaa
     3481 ccaatacttc agccacggtt gcagcgtgct cacgttttaa cccagcctgg cagagtttat
     3541 tctcgattag ctggtggagt gtttcccgac taattttcat ctgtcttcct ttttaacgac
     3601 ggtgtgaagc atgactgcaa ttaacataca gggaaaatat ctggattatg tgatccagac
     3661 aggcaaaaaa atatagttag aatttatttg ataatccgct cgcttttaac ctggttttta
     3721 aaacaaccac gcttattaaa aaataacacg taatagaatg gcggttattt gaattctttt
     3781 attaataatc cttgtgtgat attcatcacc ttatttaccc gttgtcatcg ataccgtaat
     3841 cgccacatta acactgctcg tgcaattgcc atgggtgcaa tttttaagga gttgttatga
     3901 tccacgcctt tattaaaaaa gggtgttttc aggattcggt cagtttaatg attatttcac
     3961 gaaaactcag cgaatcagaa aatgttgatg atgtttccgt aatgatgggt acgcccgcca
     4021 ataaagcgtt attagatacc acaggtttct ggcatgacga ttttaataac gccacgccga
     4081 acgatatttg cgtggcaatt cgtagcgaag cggcggatgc ggggatcgcg caggcgatta
     4141 tgcagcagct tgaagaggcg ctaaaacaac tggcgcaggg ttcaggcagc agccaggcgt
     4201 tgacgcaggt gcgtcgctgg gacagcgcct gtcagaaatt acccgatgcc agtctggcgc
     4261 tgatttcagt ggctggcgag tatgcggcgg agctggcgaa ccaggcgctg gatcgcaacc
     4321 tcaacgtgat gatgttctcc gataacgtca cgctggaaga tgaaatccaa ctcaaaaccc
     4381 gcgcgcggga aaaaggcttg ctggtgatgg ggccggactg cggtacatcg atgattgccg
     4441 ccacgccgct ggcttttgct aacgtgatgc cggaaggcaa cattggcgtc attggcgctt
     4501 ccggtaccgg gattcaggag ctgtgctcac agattgcgct ggcaggggag ggaattactc
     4561 acgcgattgg ccttggcggg cgcgacctca gccgtgaagt gggcggcatc agtgcgctga
     4621 cggcgctgga aatgctcagt gcagacgaga aaagcgaagt gctggcattt gtttcaaaac
     4681 cacctgccga agctgtgcgt ctgaaaattg ttaatgccat gaaagcaacc ggcaaaccga
     4741 cggtggcgct gtttttaggt tataccccgg cggtggcacg cgacgagaat gtctggtttg
     4801 cctcctcgct ggatgaggcc gcacgcctgg cttgcctgct ttcacgcgtt acggcgtgac
     4861 gtaacgcgat aacgcctgcc agcagcggat ttatttgcgg tttgtatacc ggcggtacgc
     4921 tggctgccga agcggcggga ttacttgccg gataccttgg cgtggaagtc gatgatgccc
     4981 atcatcacgg catgatgctc gacgccgatg gtcaccagat tattgacctg ggcgatgatt
     5041 tctacacagt cgggcgtccc catccgatga tcgacccaac cttacgcaac cagttaattg
     5101 ccgatctcgg cgctaaacca caagtgcgcg tattgctact tgatgtcgtg attggcttcg
     5161 gtgcgactgc cgatcctgcc gcctcgctgg taagcgcctg gcaaaaagcc tgtgccgcgc
     5221 gttcagataa tcaaccactg tatgccattg ccacggtgac aggcactgaa cgtgacccgc
     5281 aatgccgctc gcagcaaatc gccacgctgg aagatgcggg gatcgcggta gtgagttcgc
     5341 tgccggaagc caccttgctg gcggcagcgt taatttatcc gctctcgcct gccgcacagc
     5401 aacatacacc gtcattactg gaaaacgtcg ccgtgattaa catcggatta cgcagctttg
     5461 cgctggagct acaaagcgcc agcaaaccgg ttgtgcatta ccaatggtcg ccagtcgccg
     5521 gcggcaataa aaaactggct cgtttattag aacgtttgca ataaggggtt cccatgttta
     5581 catcagtggc gcaagccaat gctgcggtta tcgaacaaat tcgtcgtgct cgtccacact
     5641 ggctggatgt gcaaccggct ttttcactta tcagcgaact aaacgagggc aaaacactgc
     5701 ttcacgccgg gccgccaatg cgctggcagg agatgaccgg acccatgaaa ggggcgtgcg
     5761 tgggcgcatg tctgttcgaa ggctgggcga aagatgaagc gcaggcactg gcgatgctgg
     5821 agcaggggga agtgaacttc attccttgtc accatgtgaa tgccgtcggg ccaatgggcg
     5881 gcattacttc tgccagtatg ccgatgctgg tggtcgagaa cgtgaccgac ggcaaccggg
     5941 cgtactgcaa cctcaacgaa ggtatcggca aagtgatgcg ttttggcgct tacggcgaag
     6001 atgtcctgac tcgccatcgc tggatgcgcg atgtgttaat gccagtatta agcgcggcgc
     6061 tggggcgcat ggagcgcggt atcgatctca cggcgatgat ggcgcagggc attacgatgg
     6121 gcgatgagtt ccatcaacgc aatattgctt cctcagcact gttaatgcgt acgctggccc
     6181 cacaaattgc tcgcctcgat catgataaac agcacattgc ggaagtgatg gatttcctca
     6241 gcgtgaccga tcagttcttc ctcaacctcg cgatggccta ctgcaaggcg gcgatggatg
     6301 ctggcgcgat gatccgcgca ggcagcatcg ttacggctat gacccgcaac ggcaatatgt
     6361 tcgggattcg ggtaagcggg ctgggtgaac gctggtttac tgcgcctgta aacactccgc
     6421 aaggtctgtt tttcaccggc ttctcgcagg agcaggcgaa cccggatatg ggcgatagcg
     6481 cgattaccga aacctttggt atcggaggtg cggcaatgat cgcagcgcct ggcgtaacgc
     6541 gctttgtcgg tgcgggtggc atggaagcgg ccagggcggt atctgaagag atggcggaaa
     6601 tttaccttga acgcaatatg cagttgcaga tccccggctg ggattttcag ggcgcgtgcc
     6661 tggggctgga tattcgtcgc gtggtagaaa ccggcattac gccactcatc aataccggta
     6721 tcgcccataa agaggcgggg atcgggcaga ttggcgcagg caccgtgcgg gcaccgctgg
     6781 cgtgctttga acaggcgctg gaagcactgg ctgaaagcat ggggattggt tgaggaacgc
     6841 gcaatgacga tcatccatcc tctgcttgcc agcagtagcg caccgaatta tcgccagtcc
     6901 tggcggttag cgggagtgtg gcggcgggcg attaacctga tgacggaaag cggcgaactg
     6961 ttaacgctgc atcgtcaggg tagtggtttc ggccccggag gatgggtgct tcgccgcgcg
     7021 caattcgatg cgttatgcgg tggattatgc ggcaatgaac gaccacaggt tgtggctcaa
     7081 gggattcgcc tcgggcgttt cacggttaaa cagccacagc gttattgttt gctgcgtatt
     7141 acgccgcctt cgcatcctca accgcttgca gctgcatgga tgcaacgcgc ggaggaaacc
     7201 ggacttttcg ggccactggc gatggcggca agcgatccgc tgcccgctga attacgccag
     7261 tttcgtcact gttttcaggc cgcgctcaat ggcgttaaga ccgactggcg gcactggctg
     7321 ggtaaaggcc ccggattaac gccaagtcat gatgacacgt tgagcggaat gctgctggcg
     7381 gcctggtatt atggcgcttt agatgcgcgc gccggtcgcc cgttttttgc ctgttccgac
     7441 aatcttcaac tcgttaccac agcggtgagc gtcagttatt tacgttatgc cgcgcaagga
     7501 tatttcgcct cgccactcct gcactttgtc catgctctga gttgcccgaa acgtaccgct
     7561 gttgcgattg attcgctgct ggcgctgggg catacgtcag gggcagatac gctgctgggg
     7621 ttctggcttg gccaacaatt attacaagga aaaccatgaa aacactggtt gtagctcttg
     7681 ggggcaacgc cttactccag cgcggtgagg cgctgacggc ggaaaatcaa tatcgcaata
     7741 tcgccagtgc tgtacccgcg ctggcacgcc tggcccgttc ttatcggctg gcgattgttc
     7801 acggcaacgg gccgcaggtg gggctgctgg cgttacagaa tctggcgtgg aaagaggtag
     7861 aaccgtatcc gctggatgtg ctggtggcgg aaagccaggg gatgattggc tatatgctgg
     7921 cgcagagctt gagcgcacag ccgcagatgc cgcctgttac gacggtgctg acgcgcattg
     7981 aggtttcgcc tgatgatccg gcgtttttgc agccagagaa atttattggt ccggtttatc
     8041 agccagaaga acaagaggca ctggaagcgg cttacggctg gcagatgaaa cgtgatggta
     8101 aatatttgcg ccgggtggtg gcgtctccgc aaccgcgtaa aattctcgac agcgaagcca
     8161 tcgagttgtt gctcaaagag gggcatgtgg tgatttgcag tggcggcggc ggtgtgcctg
     8221 tgacggaaga tggggcaggg agtgaagcgg tgattgataa agatctcgcc gctgcgttgc
     8281 tcgccgagca gattaatgcc gatggactgg tgatcctcac cgatgctgat gcggtatatg
     8341 aaaactgggg aacgccgcag caacgtgcca ttcgccatgc cacaccggat gagttagcgc
     8401 catttgccaa agccgatggt tcgatggggc cgaaggtaac ggcggtgagt ggttatgtca
     8461 gaagccgtag taaacccgcg tggattgggg cgttatcgcg aattgaagag acgctggcgg
     8521 gcgaagcggg gacctgtatt tcgctgtagt gatgagagca tccggcaatc ggtgcaccat
     8581 gcctgatgcg acgcttgacg cgtcttatca ggcctacaat gggtaccgga gcggtaagcc
     8641 ggataaggcg tttacgccgc atccggcaat caaatgagca cctgcttaac tgaacttact
     8701 ctgcgcccaa atcacgccgc tggcatactc tggcggcagc agcgggatta aggcttccag
     8761 cgtcgcagtc agacgcgatg tgtcgctgtc ggtcaaattc agatgcccca ctttacgccc
     8821 cggacggact tctttgtcgt accagtgcag atgcaccagc ggcaatttca gccagtcata
     8881 attcacatcg ctaccaatca gattgatcat caccgacgga ttattcacta ctggttgcgg
     8941 taacggcaga tcggtaatcg cccgcagatg cagctcaaac tggctgatgc tggcaccgtt
     9001 ttgtgtccag tgaccgctgt tatgcacacg cggagccagt tcgttgatca gcagaccttg
     9061 cggggtgaca aaacactcca tcgccatcac gcccacatag cccagctcct gcataatcgc
     9121 cgacagcatc tcttcggctt gcgcctgctg ctgtgcgttg gcctgcggaa aagcgacgct
     9181 ggtgcgcaaa ataccgtcct gatgcaggtt atgcgtcagc ggataaaaca cggtgctgcc
     9241 atcaaagccg cgcgcgccaa ccagcgatac ttcaccagag aagttaatgc cctgctcgac
     9301 aatacattca ccgtaacact ctgccggtaa ctgttcggtt tcattggcgc gtaaactcca
     9361 ttgaccgcga ccgtcatagc caccagtgcg acgcttaaca atcgccagtt cacctaaacg
     9421 atcaaacacc gcaggccact cgctgcgatc ggcaagtaac tgccacggcg cggtcggcag
     9481 gtggagctta tcgaaaagct gtttctgagt cagacggtca gcaataatcg ggaacacatc
     9541 gcggttcaca aaggccggat gacgcgccag ctcgcgggtt aatgcggttt ccggccagcg
     9601 ttcgatctca gcggtaatca cgctttgttg aaaaggcacc gccgccggtt cagcgtccag
     9661 cccgaccggc cagacagcaa tgcctaacgg ttcgcctgcc tgacgcagca tacggcctaa
     9721 ctgcccgtta ccgaggacgc aaacctgttt catgccgcac ctcgcgggtc cgggttttcc
     9781 agcacttcgt cggtctgggc tttgcgccag tcattcagac gctggtgtag ctctttatcg
     9841 tgagtcgcga gaatttgtgc tgccagtaat gccgcgtttg ccgcgccagc tttaccaatc
     9901 gccagcgtac ccaccggaat gccgcgcggc atttgtacga tggagtagag gctatcgaca
     9961 ccgctcagtg cggcgctctg tactggcacg cccagcaccg gcaccagcgt tttggcggca
    10021 atcatgcctg gcaggtgcgc tgcgccgcct gcgcccgcaa taatcacctg ataaccgttc
    10081 tcttcggcgc tttcggcgaa gctgaacagt ttatcggggg tgcggtgagc agaaaccact
    10141 tcaacgtggt gcgggacatt caggatttcg aagatttcga cggcgaactg catggtagcc
    10201 cagtcgcttt tggaccccat cacgatggcg acacgcgccg gattattgcg ggaagacatg
    10261 cgtcttaaaa ctcctgtggt gcacaactct cggctttaga gggcacagag aatagcacgg
    10321 aaagagagca aggaaaacgg ttgcgtggct gtgaaatcag caaagttgtg ggttttttaa
    10381 aacggaaaat gaatcagctc aacgtcatcc gccgtgactt tcaccatcga accttccgta
    10441 tgccaggcac ccagtaccac gcgaaaagca ggttgctggt tggcgataag ttcatgcacc
    10501 gccgggcgat gggtatgccc gtggatcagc cattgcacct gatgtttttc catcgcactg
    10561 accacagcgt tttggttaac gtccatgatc gccagcgatt tactgctgtt ggcttctttg
    10621 ctgttcgcgc gcattcgcgc ggcaatgcgt ttgcgcacaa acaacggcag ggcgaggaat
    10681 agcatttgca gccagggttt gtggactttg gcgcgaaaag cctgataacc cgcgtcatcg
    10741 gtgcacagcg tgtcgccatg cataatcaac acccggcgac cataaagttc gagcaccttt
    10801 tcttccggca ataatgtcat gccgctttca cgggcaaagc gtttgccgag cagaaaatca
    10861 cggttgccat gaatgaaata acagggaacg ccagaatcgg acaccgcttt gatcgctgcc
    10921 gccatctggc gatggagtgg gttgggatcg tcgtcgccaa tccatgcttc aaacagatcg
    10981 ccaagaatat acagcgcgtc ggccttgcgg gcttcccccg ctaaaaaacg cagaaaaccg
    11041 gcggtgatcg ccggttcttc cacgcagaga tgaagatctg caataaagag tgtcgccacg
    11101 attactcgct aacggtcacg ctttcaatga taacgtcttc ttttggcacg tcctggtgca
    11161 taccgttacg accggttgca acacctttga ttttgtctac cacgtccatg ccgtcaacca
    11221 cttcagcaaa cacgcagtag ccccaacctt gcaggctttc gccagagaag ttcaggaagt
    11281 cgttatcaac cacgttgatg aagaactgtg cagttgcaga gtgcggagcc tgagtacgtg
    11341 ccattgccag cgtaccacgg gtatttttca ggccgttgtt ggcttcgttt ttgatcggtt
    11401 ctttggtggc tttttgtttc atgcccggtt caaaaccgcc gccctgaatc ataaagccgt
    11461 tgataacacg gtggaaaatg gtgttgttgt aaaaaccttc gcggcagtag tccaggaagt
    11521 ttttaactgt ttcaggtgct ttatcgtcaa aagttttgat gacaatatcg ccgtgattgg
    11581 tgtggaaagt aaccattttt gcatcctgtt ccgtttgatt ggtgcttcaa cccagttcgg
    11641 gtcatatata gggtggtgtt atagcataac cgcacgatcg gatcatcacg caatgtatgc
    11701 tgattcgcgc gggaaatatg ggtattatac gcaactcaat tacccacaca tgtctaaacg
    11761 gaatcttcga tgctaaaaat cttcaatact ctgacacgcc aaaaagagga atttaagcct
    11821 attcacgccg gggaagtcgg catgtacgtg tgtggaatca ccgtttacga tctctgtcat
    11881 atcggtcacg ggcgtacctt tgttgctttt gacgtggttg cgcgctatct gcgtttcctc
    11941 ggctataaac tgaagtatgt gcgcaacatt accgatatcg acgacaaaat catcaaacgc
    12001 gccaatgaaa atggcgaaag ctttgtggcg ctggtggatc gcatgatcgc cgaaatgcac
    12061 aaagattttg atgctttgaa cattctgcgc ccggatatgg agccgcgcgc gacgcaccat
    12121 atcgcagaaa ttattgaact cactgaacaa ctgatcgcca aaggtcacgc ttatgtggcg
    12181 gacaacggcg acgtgatgtt cgacgtcccg accgatccaa cttatggcgt gctgtcgcgt
    12241 caggatctcg accagctgca ggctggcgcg cgcgttgacg tggttgatga caaacgcaac
    12301 ccgatggact tcgttctgtg gaagatgtcg aaagagggcg aaccgagctg gccgtctccg
    12361 tggggcgcgg gtcgtcctgg ctggcacatt gaatgttcgg ccatgaactg caagcagctg
    12421 ggtaaccact ttgatatcca cggcggcggt tcagacctga tgttcccgca ccacgagaac
    12481 gaaatcgcgc agtccacctg tgcccatgat ggtcagtatg tgaactactg gatgcactcc
    12541 ggaatggtga tggttgaccg cgagaagatg tccaaatcgc tgggtaactt ctttaccgtg
    12601 cgcgatgtgc tgaaatacta cgacgcggaa accgtgcgtt acttcctgat gtcgggccac
    12661 tatcgcagcc agctgaacta cagcgaagag aacctgaagc aggcgcgtgc ggcgctggag
    12721 cgcctctaca ctgcgctgcg cggcacagat aaaaccgttg cgcctgccgg tggcgaagcg
    12781 tttgaagcgc gctttattga agcgatggac gacgatttca acaccccgga agcctattcc
    12841 gtgctgtttg atatggcgcg tgaagtgaac cgtctgaaag cagaagatat ggcagcggcg
    12901 aatgcaatgg catctcacct gcgtaaactt tccgctgtat tgggcctgct ggagcaagaa
    12961 ccggaagcgt tcctgcaaag cggcgcgcag gcagacgaca gcgaagtggc tgagattgaa
    13021 gcgttaattc aacagcgtct ggatgcccgt aaagcgaaag actgggcggc ggcagatgcg
    13081 gcacgtgacc gtcttaacga gatggggatc gtgctggaag atggcccgca agggaccacc
    13141 tggcgtcgta agtaattgcg caattgccgg atgcgagttt tcgcatccgg ttatcgtctg
    13201 cgccaccaca acattcccat cagtagcatc cccggcaacc acacccacat caattcggaa
    13261 ataatcacct gatgcccgta cgctgtggtg taacgagaca atgcaaacgg cgcgacttta
    13321 atcacctgcc agggagcgaa aaagcgttca tctgaccacg gccacagcca gccaacgccc
    13381 ttaccgccag tggttaccga atccagtaag ctgtgcgata gcaacgagac ggttaaaaac
    13441 agccagcagc gaatcagccc agccctgaac catcgacgtc caatcagcac acataagagc
    13501 gggacaacaa acgcaaacac cagcgaatgg gtaaacccgc gatgaccaaa aacattgccg
    13561 taagcaacgc caaatttaaa cgacaatacg tcggcatcgg gcagcatcgc caggatgatt
    13621 ccggcaaata acagacgcgg agggatgact ttcgaaccca accctaaacc aatgcaaagg
    13681 ggaacggcgg cgtgcgtaat aacggttggc atgatggtcg cttcggcaaa atgtcgatgc
    13741 tatcagcatg gatgaacggg gcgtagaggg caaaagtctg aaaagagaac cggcctgttg
    13801 atacaggccg ggaaagggat caggcaacaa cctgtacgct gtgtcctgca aaactcactg
    13861 tctgaccggc gacgattttg cagcgtttgc gcgtttcaac cgcaccgtcg actttcacct
    13921 ggccttcggc aatcgcgatt ttcgcctgcg cgccgctttc gctccagcct tccagtttca
    13981 gcaagtcgca cagctcaacg tgcggatgtt tacctaaaga aaatgtcgcc atgttactca
    14041 ccctgtggat catgatattc aacgcacgcc tgtagcgtgt tttcaatcag cgtggcaacc
    14101 gtcatcgggc caacgccgcc gggaacaggc gtaatgtatg aggcgcgttt agccgcgtct
    14161 tcaaacacga cgtcgcccac aactttgcca ttttccagac ggttgatgcc gacatcaatc
    14221 acaattgcgc cttctttgat ccagtcaccg ggaataaagc ctggcttgcc aacggcaacg
    14281 atcaacagat cggcattttc tacgtgatga cgcagatttt tagtgaagcg gtgagtcacg
    14341 gtggtggtgc aacctgcgag caacagttcc atgctcattg ggcgaccgac gatattcgat
    14401 gcgccaatca ccacggcgtt gaggccgaag gtatcaatgt tgtaacgctc aagcagcgtg
    14461 acgataccgc gcggggtgca gggacgcaga cgcggcgcgc gctggcacag acgaccgacg
    14521 ttgtaaggat ggaaaccgtc cacgtctttg tccggatgaa tacgttccag cactttgacg
    14581 ttatcaatac ccgccggtaa cggcagttga accagaatgc catcgatggt gttgtcggca
    14641 ttcagcgcat cgataagctc cagcagctcc gcttcgctgg tggtttccgg gaggtcataa
    14701 gagcgggaga cgaacccgac ttcttcacaa gccttgcgtt tgcttgcgac ataaatttgc
    14761 gatgcagggt tactacccac cagcacaacg gccagtcctg gtgcccgcag tctggctgca
    14821 atacgagcct gaactttttg agcaacttca gagcgcacct gctgcgcaat cgttttaccg
    14881 tcaataatct ttgctgccat cagagagaga attccatctg ttacgtaggt cgaaggggat
    14941 gcgtctattt tgtcagaagc ggggcgcgct gtcaggtttc gtttcagatt tatcgcgtga
    15001 agcgacctct tgcgaaggtt aggcgcaccg tcgctgaggt cgctgagatt gaaagcttca
    15061 tttttcgtac atgatggcgt tgtaaatctg gaactgattt atttccttgt ttaaggatta
    15121 agataat
//
LOCUS       DADLSB010000098        15049 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.098, whole genome shotgun sequence.
ACCESSION   DADLSB010000098 DADLSB010000000
VERSION     DADLSB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.098"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..238
                     /locus_tag="JN331_002782"
     CDS             <1..238
                     /locus_tag="JN331_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY7260887.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="JN331_002783"
     CDS             248..886
                     /locus_tag="JN331_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY7260888.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNSYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="JN331_002784"
     CDS             889..2052
                     /locus_tag="JN331_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7260889.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..3761
                     /locus_tag="JN331_002785"
     CDS             2136..3761
                     /locus_tag="JN331_002785"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY7260890.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASSPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3878..4153)
                     /gene="yjfN"
                     /locus_tag="JN331_002786"
     CDS             complement(3878..4153)
                     /gene="yjfN"
                     /locus_tag="JN331_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY7260891.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4302..4631)
                     /gene="bsmA"
                     /locus_tag="JN331_002787"
     CDS             complement(4302..4631)
                     /gene="bsmA"
                     /locus_tag="JN331_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY7260892.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4813..5562
                     /gene="yjfP"
                     /locus_tag="JN331_002788"
     CDS             4813..5562
                     /gene="yjfP"
                     /locus_tag="JN331_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7260893.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGALMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5559..6314)
                     /gene="ulaR"
                     /locus_tag="JN331_002789"
     CDS             complement(5559..6314)
                     /gene="ulaR"
                     /locus_tag="JN331_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY7260894.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6422..7486)
                     /gene="ulaG"
                     /locus_tag="JN331_002790"
     CDS             complement(6422..7486)
                     /gene="ulaG"
                     /locus_tag="JN331_002790"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY7260895.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGKALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7841..9238
                     /gene="ulaA"
                     /locus_tag="JN331_002791"
     CDS             7841..9238
                     /gene="ulaA"
                     /locus_tag="JN331_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY7260896.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIISFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDNYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9254..9559
                     /gene="ulaB"
                     /locus_tag="JN331_002792"
     CDS             9254..9559
                     /gene="ulaB"
                     /locus_tag="JN331_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY7260897.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9569..10033
                     /gene="ulaC"
                     /locus_tag="JN331_002793"
     CDS             9569..10033
                     /gene="ulaC"
                     /locus_tag="JN331_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY7260898.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10047..10697
                     /gene="ulaD"
                     /locus_tag="JN331_002794"
     CDS             10047..10697
                     /gene="ulaD"
                     /locus_tag="JN331_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY7260899.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10707..11561
                     /locus_tag="JN331_002795"
     CDS             10707..11561
                     /locus_tag="JN331_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAY7260900.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ECLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            11561..12247
                     /locus_tag="JN331_002796"
     CDS             11561..12247
                     /locus_tag="JN331_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HAY7260901.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12376..12651)
                     /locus_tag="JN331_002797"
     CDS             complement(12376..12651)
                     /locus_tag="JN331_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7260902.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12978..13373
                     /gene="rpsF"
                     /locus_tag="JN331_002798"
     CDS             12978..13373
                     /gene="rpsF"
                     /locus_tag="JN331_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY7260903.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTVAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13380..13694
                     /gene="priB"
                     /locus_tag="JN331_002799"
     CDS             13380..13694
                     /gene="priB"
                     /locus_tag="JN331_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY7260904.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13699..13926
                     /gene="rpsR"
                     /locus_tag="JN331_002800"
     CDS             13699..13926
                     /gene="rpsR"
                     /locus_tag="JN331_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY7260905.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13968..14417
                     /gene="rplI"
                     /locus_tag="JN331_002801"
     CDS             13968..14417
                     /gene="rplI"
                     /locus_tag="JN331_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY7260906.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEVRRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14488..15045)
                     /locus_tag="JN331_002802"
     CDS             complement(14488..15045)
                     /locus_tag="JN331_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7260907.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLR
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3512 a         3659 c         4066 g         3812 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatagct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacgttttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaacc gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcacgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccaata acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggttccggca
     3421 atattatgtg cctggatgtg ttgcgcgttc tcaataagca agcgggcgta tacgacttat
     3481 tgtcggaagc atttgtggaa gtgaaagggc aggatcgcta ttttgatcgc gcggttcgtc
     3541 gtttacagca gcagctgcgt aagccagctg aagaactggg gcgagagatt actcatcagc
     3601 tattcctgct gggctgcggt gcgcaaatgt tgaaatatgc ttcttcgcca atggcgcagg
     3661 cgtggtgtca ggtgatgtta gatacgcgcg gcggcgtacg gttgtcagag cagatccaga
     3721 atgatttatt gctgcgggcg acggggggag tgtgtgtgta agcgtatacg actgatgcga
     3781 cgctggtttc gattaactaa atgaaatatg tgaaaattgt aggccggaca aggcgctcgc
     3841 gccgcatccg gcattgttca tttgctgtag atgacactca tgcatacagt atcgcctgta
     3901 cgcgccagtt atcaggctgc tggtcttcgt acatggtaat aatgcggtac catgacgcac
     3961 cttgttcatc ggcctttaac gccacgatac gttccacgtc ctgcggatcc cctgaaatat
     4021 tgctaactga gatctgacct atttcattca ggcccgtcac gcagtcagca ctggcgaatt
     4081 ctgcagactg tgccgtggcg ctcgcaggat aagtcactaa ttgcagcgag ggtgaggcaa
     4141 gtaattgttt catcgtcagc tccatttttc gtttccccac atgttgcagg gcattccatc
     4201 gcggtaataa acgcccaagc ttccagccgg taatttattg tgcacaggta aacgcatagg
     4261 gacgcaaagc aatgtaaagc tgactatgta agttgtctga tttatttacg gtacaaaatg
     4321 gcttgtgaat accactgtcc tgtcacgatg gtttcgtcca ccatgacgac aacgtaataa
     4381 tcagcttttg cagcgacagc ttttgctctg atttctgcta atgcgtcatc cggagaaccc
     4441 cgaaccatcg tgcttacgct acctattcgc tgtaaccctt gcgtctggtc gcggcgaatc
     4501 tcttgcggat ggtccgttac tggcggtgct ggctgtggcg taccttgcag tgcgctacag
     4561 gcacttaaca tcaacaccaa taataaactg gcaaaccggt aaataacgct attacgtttc
     4621 ctgctaacca tagtgtagtg ccttattaat ttaactttgg gggaatgttc ccgaattgtt
     4681 acctggtacg cgattttcga atgaaaacgt gacgaaagcg taacccacat ctcaacatta
     4741 tgaactaata ccgccagcag aaacttgtgc taaggatttc ttcatgtcat accagacaaa
     4801 aaggagagac agatgattga aatagaatca cgcgagctgg cagatattcc cgttcttcat
     4861 gcttatcctg tcgggcaaaa agataccccg ttaccgtgcg taatttttta tcacggcttt
     4921 acttcatcca gcctggtgta tagctatttt gccgttgcgc tggcgcaggc tggtttgcgg
     4981 gtgatcatgc cggatgcgcc cgatcacggt agccgtttta gtggcgacga agcgcggcgg
     5041 ttaaatcaat tctggcaaat cttgctacaa agtatgcagg aattcactac tttacgtgcg
     5101 gcaatagccg aagaaaactg gctgcttgat gaccgtctgg cagtcggtgg cgcgttgatg
     5161 ggcgcgatga cggcactggg aattaccgct cgccacccca cggtgaaatg taccgccagc
     5221 atgatgggat cgggctattt tacatcgctc gcccgttcac tgtttccacc gctgatacct
     5281 gaaacggcag cacagcagaa tgaattcaat aaaattgtcg cgccgctggc agagtgggaa
     5341 gcgacaaacc acctggaaca acttggggac agacctctac tgctgtggca tggcctcgac
     5401 gatgacgttg tgcctgccga cgaatcacta cgtttgcagc aggccttaag cgagacggga
     5461 cgggataaac tgctaacctg ttcatggcag ccaggcgtgc gtcaccgcat tacgcctgag
     5521 gcgttagatg ctgccgtgac atttttccgc cagcatcttt aaacacgcag aatgctgacc
     5581 ccttgcgctt ccagttgttg caggatttcc gggttagcat ttttgccggt gatcagcata
     5641 tcgatttgat cggcacggct aaaaagcatt cccgcgcgtt cgccaatctt actgctatca
     5701 accagtacca ccagtttccc taccacgctc agcatcttct gctctgccat tgctgtcagc
     5761 atatcggttt tatacaaccc ttctgcggtc agcccttttc cgctggtaaa catccagtgc
     5821 ccggcataga gactgttttc gctgccctgc gggcttaaag tgatggactg acttttgttg
     5881 tactgtccac ccataatgat cacgctgtca tgttcttgat cgatcaggta atttgccagc
     5941 ggtagataat tagtgatgat ttgcactggc ttgccacaca tttcccgccc aagcagaaac
     6001 gcggtggagc cgcagttgat gactacgctt tcgcccggat taaccagctg cgaggccgct
     6061 ttagcaatac gtactttttc atcgtgattc tgcgcctgat gcagattcat cggcgtccag
     6121 cgcgggcgct gttgggtaat agcttctgcg ccattgcgca cctttttcag tttgccgctt
     6181 tcgccaagtt tattgatatc gcgtcgcgca gtggcaggcg aaattcccag acgctcaacg
     6241 actttctcaa cggtcacaaa gcccaattgt gcgagcattt ctaggaggat ttgatgtctt
     6301 tgtgcttcag tcatgagcta ttccgataaa atttgatttt tttagatgat atttgaaata
     6361 gccaggaaat actacgccgg atagcgcgaa cttctccacg ctatccggta taaacgacag
     6421 attacaggaa tgacttgaac ggcagatccg gttcaatagt gaagcaatca tcgaaaccgc
     6481 gcggatagtg gtactcgaag ttgtctttat ccagcggcca ggtaaatttg ccgccaacct
     6541 gccagataaa cggcttgaag ccatacttca gacgatcttt tttcatctcc cacagcacgc
     6601 ggatctcttg cggatcggcc tggaagtttg accagatgtc gtggtggaac gggataacca
     6661 ctttcgcatt cagggcttta cccatacgca gcatatcggc gctggtcatt ttgtcggtaa
     6721 taccgcgcgg gttctcacca tacgatccta acgccacatc gatctgatgt tcgttaccgt
     6781 gcttcgcata atagttagag tagtgggaat cgccactgtg atatagggaa ccgccagggg
     6841 ttttgaacag gtagtttact gcgcgatcgt ccatgccatc tggcagtacg ccagccgctt
     6901 tttgatcggc aggcagggtg atcagtgcag tacggtcgaa agcgtcaagc gcatgaattt
     6961 caatgtcttt cacttttaca acatcgcccg gtttgaccac gatgcaacgc tctttcggta
     7021 cgccccagcc aatccacaaa tcaacgcagg ttttcggtcc aatgaatggt acgtcatcag
     7081 cacagttctg catcacggca gcagcgacgt taacgtcgat atgatcgttg tgatcgtgag
     7141 tcgccagtac cgcgtcgatc tggcgaatcg caaacggatc aagaacaaac ggggtggtgc
     7201 gtaggtttgg ctgcagtttt ttcacgccag ccatgcgctg catctggtga ccctgtttca
     7261 ttaacgggtt accgtgactt tgtttgccag tgccgcacca gaaatcaacg caaacgttgg
     7321 tgccaccttc tgatttcaac cagatcccgg tgcagcctag ccaccacatc gcaaatgtgc
     7381 caggggcgac ctgctcttgt tcaatttctt cattcagcca gctaccccac tccgggaaag
     7441 tgctcaggat ccaggattct cgggtgatac ttttcacttt actcatcgcc atttaccttc
     7501 atgatagttc aattcgaatc aatatgtgat tggttttgat taatcctgac actatttttt
     7561 caggaaggca atgaccattt tttgactttt tgccagggaa gttgttgttg attttgagtg
     7621 tggaaagatt taatggaatg tgtagttaaa ttaactaaat gaatttaaat aaataattgt
     7681 ttcgttgtgt gaatcccgct ctatccgtgt ggaattattt gcgggtcgcg tcacatttaa
     7741 tcataaataa tcttgttgtg attacttttg aaaattagag tgagtgcaca acattccggg
     7801 tgtgcggaat acccggttac ctcttcttca ggagatcgtt atggagatcc tctacaacat
     7861 ctttaccgtg ttttttaacc aggtcatgac caatgccccg ttgttgctgg gtattgtgac
     7921 ctgtctgggc tacatcctac tgcgcaaaag tgtcagcgtt attattaaag gcacgattaa
     7981 aaccataatt agtttcatgt tgttgcaggc agggtccggc atcctcacca gcaccttcaa
     8041 accggtggtg gcaaaaatgt ccgaagtcta cggcattaac ggcgcaattt ccgataatta
     8101 cgcgtcgatg atggcaacca tcgaccgcat gggcgatgcc tatagctggg taggttacgc
     8161 ggtgttgtta gcgctggcgc tgaacatctg ttacgtgctg ctgcgtcgca ttaccggtat
     8221 tcgtaccatc atgctgactg gtcatatcat gttccagcag gcggggctga ttgccgttac
     8281 gttgtttatt ttcggctact ccatgtggac aaccattatc tgcacggcga ttctggtttc
     8341 gctctactgg ggcattacct ccaacatgat gtacaagccg actcaggaag tgacggacgg
     8401 ctgcggtttc tccatcggtc accagcaaca gtttgcatca tggattgcct ataaagttgc
     8461 gccgttcctc ggcaaaaaag aggaaagcgt tgaagacctc aaactgccag gctggctgaa
     8521 tattttccac gacaacatcg tctccacggc gattgtcatg accatcttct ttggtgccat
     8581 tctgctctcc ttcggtatcg acaccgtgca ggcgatggca ggcaaagtgc actggacggt
     8641 ctatatcctg caaaccggtt tttccttcgc ggtggcgatc ttcatcatca ctcagggtgt
     8701 gcgcatgttt gtggcggaac tctctgaagc gtttaacggt atctcccagc gcctgatccc
     8761 tggtgcggtt ttggcgattg actgtgcggc tatttatagc ttcgcgccga acgccgtggt
     8821 ctggggcttt atgtggggca ccatcggtca gctgattgcg gttggcatcc tggtcgcctg
     8881 cggttcctcg atcctgatta tcccaggctt tatcccgatg ttcttctcca acgccaccat
     8941 cggcgtgttc gctaaccact tcggcggctg gcgtgcggcg ctgaagattt gtctggtgat
     9001 ggggatgatc gaaatctttg gttgcgtctg ggcggtgaaa cttaccggta tgagtgcctg
     9061 gatggggatg gcggactggt cgattctggc accgccgatg atgcagggct tcttctccat
     9121 cggtatcgcc tttatggccg tcatcattgt aattgcactg gcttatatgt tcttcgctgg
     9181 ccgcgcactg cgcgcagaag aagatgcaga aaaacaactg gcagaacagt ctgcttaata
     9241 aggagttttg attatgaccg tacgtattct ggctgtgtgt ggcaacggac aaggcagttc
     9301 catgatcatg aagatgaaag tggaccagtt tttaacccaa tcaaacattg accacacggt
     9361 aaatagctgc gcggttggcg aatacaaaag cgagttgagc ggcgcggata tcatcatcgc
     9421 ttctacgcac attgcgggcg aaatcaccgt gaccggcaac aaatacgtgg ttggcgtgcg
     9481 caacatgctc tctcctgccg actttggccc gaaactgctg gaagtgatca aagcgcattt
     9541 cccgcaggat gtgaagtaag gacgcgccat gaaattacat gattcgctgg cggagaataa
     9601 atccatccgc ctgcaggctg aagctgagac atggcaggac gcagtaaaaa tcggcgttga
     9661 cctgctggtg gcggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt
     9721 tgaacagcac ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc
     9781 ggaagagggc gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt
     9841 caaccacgaa gataacgacc cggtggatat cctcatcacc atggcggcgg tcgatgccaa
     9901 cacgcaccag gaagtgggca tcatgcagat cgtcaacctg tttgaagatg aagaaaattt
     9961 cgaccgttta cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa
    10021 cgcggcagct taagaaggaa ttgaagatgt cattaccgat gttgcaagtc gcgctggaca
    10081 accagactat ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta
    10141 tcgaagtggg taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag
    10201 cgctctaccc gcacaaaatc gtgctggcag acgccaaaat tgccgatgcg ggcaaaattc
    10261 tttctcgtat gtgcttcgaa gccaacgctg actgggtgac cgtgatttgt tgtgcggata
    10321 tcaacaccgc caaaggtgcg ctggacgtag caaaagagtt taacggcgac gtgcagatcg
    10381 aactgaccgg ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggtattcagc
    10441 aggtggttta tcaccgcagc cgtgacgcgc aggccgctgg cgtggcgtgg ggtgaagccg
    10501 atatcactgc gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc accggtggcc
    10561 tggcgctgga agatctgccg ctgttcaaag gcattccaat tcatgtgttt atcgccggtc
    10621 gcagcatccg tgatgccgct tctccggttg aagccgcgcg tcagttcaaa cgttccatcg
    10681 ccgaactgtg gggctaagga gcggatatgt tgtccaaaca aatcccgctt ggcatctatg
    10741 aaaaagcgct ccccgccggg gagtgctggc tggaacgcct gcgactggca aaaacgttgg
    10801 gcttcgattt tgtcgagatg tcggtagatg aaactgacga gtgcctgtcg cgcctcgact
    10861 ggagccgcga gcagcgtctg gcgctggtga atgcgattgt tgaaaccggc gtgcgtgtgc
    10921 cgtccatgtg cctttctgcc catcgtcgtt tcccgttggg cagcgaagat gacgcggtgc
    10981 gggcgcaggg gctggagatt atgcgtaaag ccatccagtt cgcccaggac gttggcattc
    11041 gcgtgatcca gctggcgggc tatgacgttt actatcagga agccaataac gaaacgcgtc
    11101 gtcgtttccg tgacggcctg aaagagagcg ttgagatggc gagccgcgcg caggtgacgc
    11161 tggcgatgga gatcatggat tatccgttga tgaactccat cagcaaggcg ctgggttacg
    11221 cgcactatct caacaacccg tggttccagc tctacccgga tatcggcaac ctgtcggcgt
    11281 gggacaacga tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc gcggtgcatg
    11341 tgaaagacac caaaccgggc gtcttcaaaa acgtgccatt tggcgaaggt gtggtggatt
    11401 tcgaacgttg cttcgaaacg ctcaaacaga gtggctattg cgggccgtac ctgattgaga
    11461 tgtggagcga aacggcggaa gacccggcgg cagaagtggt gaaagcgcgt gattgggtga
    11521 aagcgcgcat ggccaaagca ggcatggtgg aggcggcata atgcaaaagc taaaacagca
    11581 ggtgtttgaa gccaacatgg atctgccgcg ctatgggctg gtgaccttta cctggggcaa
    11641 cgtcagcgct atcgaccgcg aacgcgggct ggtggtgatc aagcccagcg gcgttgccta
    11701 cgaaaccatg aaagcagacg atatggtggt ggtcgatatg agcggcaagg tggtggaagg
    11761 ggagtatcgc ccatcttccg acactgcgac gcatctcgaa ctctaccgtc gttacccgtc
    11821 gcttggcggc attgtccata cccactccac tcatgccacc gcatgggcgc aggcggggct
    11881 ggcgatcccg gcgttaggca ccacgcacgc tgactacttc tttggtgata taccgtgtac
    11941 gcgtggatta agcgaagaag aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat
    12001 tatcgaaacg ctgggcaacg ccgagccgct gcatacgccg ggaattgtgg tgtatcagca
    12061 cggaccgttc gcctggggga aagatgccca cgacgcggtg cataacgcgg tagtgatgga
    12121 agaagtggcg aaaatggcgt ggattgcgcg cagtattaat ccacaactca atcatatcga
    12181 cagctacctg atgaataaac acttcatgcg taagcacggt cctaacgctt attacgggca
    12241 gaagtaaaac acgcgctgcg gaaatttcct tcctcgggag ataactggtc taattccgca
    12301 gccgtttttc aaaaaaagcc ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt
    12361 tggttatacc tggtgctagc gataaatatc cgcgctggcg tgcatattgc cgttactccc
    12421 cggttcccgc atcagaatta cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt
    12481 taatgcctga cgtgcttcgc tttcagtggc gaaattatga ttgatataaa tcacgcctaa
    12541 gctttgcaca tcgtccatat ttctggcctg gtggttatta atttcaatgg ctgcccatgt
    12601 atttgcactt agcaaaagca cagccagaag ggctaaaact cgactgaaca tagatacctc
    12661 ctcgacggct gactttgtgt gctctcctct gtgatgatct tctgatttaa ttttaatcaa
    12721 tgataaagaa gttgatggtg accatttctg atgcagttgt tcaaaaaaac actgtgacgg
    12781 aatgtgatga acttcaaatc agcggtttag aggttatttg cgaaagggga gatttatttc
    12841 tgctccgccc ttgagtttag cggggtatgt cagtactata acgcgtcatt tttcagccga
    12901 cctttaacac gttccttgcc tccccgggat tcggctgacc cagacaggag gctgaataat
    12961 ccgtaaggag caattcgatg cgtcattacg aaatcgtttt tatggtccat cctgaccaga
    13021 gcgaacaggt tccgggcatg atcgagcgct acactgttgc catcactggt gcagaaggca
    13081 agatccaccg tctggaagac tggggccgcc gtcagctggc ttacccgatc aacaaactgc
    13141 acaaagcaca ctacgttctg atgaacgttg aagctccgca ggaagtgatc gatgagctgg
    13201 aaactacctt ccgcttcaac gatgccgtta tccgcagcat ggttatgcgt accaagcacg
    13261 ctgttaccga agcatctccg atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg
    13321 atttcgcaaa cgaaaccgct gatgatgctg aagctgggga ttctgaagag taatttctga
    13381 tgaccaaccg tctggtgttg tccggcaccg tgtgcaggac tccccttcga aaggtcagtc
    13441 catcaggaat tcctcactgc cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg
    13501 gctttcaccg gcaggcatgg tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg
    13561 ccattactca cagtataacg gtcggcagtc gcataaccgt tcaggggttc atttcatgcc
    13621 acaaggcaaa gaacggactg agcaaaatgg ttttgcatgc cgagcagatt gaattgatag
    13681 attctggaga ctagccatat ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc
    13741 gcggaaggcg ttcaagagat cgactataaa gatatcgcta cgctgaaaaa ctacatcacc
    13801 gaaagcggta agattgtccc aagccgtatc accggtaccc gtgcaaaata ccagcgtcag
    13861 ctggctcgcg ctatcaaacg cgctcgctac ctgtccctgc tgccgtacac tgatcgccat
    13921 cagtaatcgg tcacggtcca ttaatacgac tttgagagga taaggtaatg caagttattc
    13981 tgcttgataa agtagcaaac ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct
    14041 atgctcgtaa cttcctggta ccgcagggta aagctgttcc agctaccaag aaaaacattg
    14101 aattcttcga agtacgtcgc gctgaactgg aagctaaact ggctgaagtt ctggcagctg
    14161 ctaatgctcg cgctgagaaa atcaatgcac tggaaactgt taccatcgcg tctaaagctg
    14221 gcgacgaagg taaactgttc ggttccatcg gtactcgcga catcgctgac gctgtaactg
    14281 cagctggcgt tgaagtggct aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca
    14341 ctggcgaaca cgaagtgagc ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa
    14401 acgtagtagc tgaataattc gttattcaac gagacgtaaa aacgccgacc attggtcggc
    14461 gttttgcttt ctatttttcg tcaggcatta atttcgcaag tagatccatt caccattctc
    14521 ttgtgtccat aagtcaggat ggtttgctgg cgttagatgc atacgatcac tccttatgtc
    14581 tcggtcctgc ggcactcgct cgaaacctaa acccgagtag attgcggctg aatttgataa
    14641 ggaacgtaga ccaattttca aatcatgggg taaatcagaa tacgcgttta gcaatgcagc
    14701 aatgatgaat ccgagggcat tagaaacgcc tgtaacagtg cgaagttggt cgatataaag
    14761 atcgattgtt ggtagttcag atgaccagca attaggcatt tgggcaggaa gggttacaac
    14821 gtatcctatc gtttggccgg tatagtctgg agtactataa ccaggtccca aaacaacctc
    14881 gtgtattgtg tatttactgt tgggttcgaa ggattcgaca tcttgcaata aatatttcag
    14941 ttctgcggtt aacctcgcac ccgcgtaagc tttattgtat ttctgggcgt gaatattatc
    15001 taatgtagct tcatggttcc atccaaaaga attatcacca ttcattgtt
//
LOCUS       DADLSB010000099        14636 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.099, whole genome shotgun sequence.
ACCESSION   DADLSB010000099 DADLSB010000000
VERSION     DADLSB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.099"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(86..2062)
                     /gene="speA"
                     /locus_tag="JN331_002803"
     CDS             complement(86..2062)
                     /gene="speA"
                     /locus_tag="JN331_002803"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY7260908.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2071..2202)
                     /gene="yqgB"
                     /locus_tag="JN331_002804"
     CDS             complement(2071..2202)
                     /gene="yqgB"
                     /locus_tag="JN331_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY7260909.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2338..2553
                     /locus_tag="JN331_002805"
     CDS             2338..2553
                     /locus_tag="JN331_002805"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260910.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2857..4011
                     /gene="metK"
                     /locus_tag="JN331_002806"
     CDS             2857..4011
                     /gene="metK"
                     /locus_tag="JN331_002806"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY7260911.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4448..5842
                     /locus_tag="JN331_002807"
     CDS             4448..5842
                     /locus_tag="JN331_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7260912.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5919..6416
                     /locus_tag="JN331_002808"
     CDS             5919..6416
                     /locus_tag="JN331_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY7260913.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6511..7218
                     /gene="endA"
                     /locus_tag="JN331_002809"
     CDS             6511..7218
                     /gene="endA"
                     /locus_tag="JN331_002809"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY7260914.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTHSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7298..8029
                     /gene="rsmE"
                     /locus_tag="JN331_002810"
     CDS             7298..8029
                     /gene="rsmE"
                     /locus_tag="JN331_002810"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY7260915.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8042..8992
                     /gene="gshB"
                     /locus_tag="JN331_002811"
     CDS             8042..8992
                     /gene="gshB"
                     /locus_tag="JN331_002811"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY7260916.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9029..9664
                     /locus_tag="JN331_002812"
     CDS             9029..9664
                     /locus_tag="JN331_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY7260917.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKISPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9664..10080
                     /gene="ruvX"
                     /locus_tag="JN331_002813"
     CDS             9664..10080
                     /gene="ruvX"
                     /locus_tag="JN331_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY7260918.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10319..11236)
                     /locus_tag="JN331_002814"
     CDS             complement(10319..11236)
                     /locus_tag="JN331_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY7260919.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTG"
     gene            11254..11958
                     /gene="yggS"
                     /locus_tag="JN331_002815"
     CDS             11254..11958
                     /gene="yggS"
                     /locus_tag="JN331_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY7260920.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11976..12542
                     /gene="yggT"
                     /locus_tag="JN331_002816"
     CDS             11976..12542
                     /gene="yggT"
                     /locus_tag="JN331_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY7260921.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQSTGNMLLPGLWMAL"
     gene            12539..12829
                     /locus_tag="JN331_002817"
     CDS             12539..12829
                     /locus_tag="JN331_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY7260922.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12837..13430
                     /locus_tag="JN331_002818"
     CDS             12837..13430
                     /locus_tag="JN331_002818"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY7260923.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13423..14559
                     /gene="hemW"
                     /locus_tag="JN331_002819"
     CDS             13423..14559
                     /gene="hemW"
                     /locus_tag="JN331_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY7260924.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3530 a         3871 c         3753 g         3482 t
ORIGIN      
        1 gcagctagat tatttttaag aatatataaa aagaaagcgg gccgatttac gtaaataaat
       61 cgacccgcca gcggaataat cttaattatt catcttcaag ataagtataa ccgtacaaac
      121 ctgcctcgaa ctcttcaagg aactgttgtt gcagttcagc atcaagatcg gttttcttca
      181 cttgatcgcg gaactgggtt aacagcgttt tcggatcgag ctgtacatat tgcagcatgt
      241 ccgccacggt atcgccttcg tcagacagtt ctacttctac gctaccgtca gggaacacga
      301 acacgtcaac tgcttcggta tcaccgaaca ggttgtgcat attgccaagg atctcctgat
      361 atgcaccgac cataaagaaa ccgagcatcg gcggattctc tggatcgtac tccggcattg
      421 gcattgtcgt ggcaatacca tcaccatcaa tatagtggtc gatagcaccg tcagagtcac
      481 aggtaatatc cagcagcaca gcgcgacgtt ccggcacttg atccagccct tccagcggta
      541 gaaccgggaa caactggtcg atcccccatg cgtccggcat tgactggaac agcgagaaat
      601 tgacgtacat tttgtccgcc atacgttcct gcagttcgtc gattatcgga cgatgcgcac
      661 ggttttgcgg atccagttgc ttctgcactt catggcacat gctcagatag agttgttctg
      721 cccatgcacg ttcttgcaag ctaaaggtgc cggaagagta gccgatatga atgtcgtgca
      781 gatccatctg gctgtcgtgt aaccattcac gcaaagaacg gcgagttccc ggttcgtgca
      841 tttcctgcca ggtttcccac atgctttgca gcgcacgcgg cgcatcttct gcgggcgcgg
      901 tcggcaccgt atattcgtta cgttccacgc cgatgatatt cgacaccagc acggtatggt
      961 gcgcagtcac cgcacggccc gattcggtga ttaccgtcgg atgcggcaga ccattttctt
     1021 cacacgcatc gccaatcgcc cagatgatgt tgttggcgta ttcattgagg ccatagttta
     1081 cggagcagtc agactgcgaa cgggtgcctt cataatccac gcccagaccg ccgccgacgt
     1141 cgaagcactg aatattgacg cccagcttgt gcagttccac atagaaacgc gcggattcac
     1201 gaacgcctgt cgcgatatcg cgaatattcg ccatctgcga accgaggtgg aagtgcagta
     1261 gttgcaggct gtcgagacgc ccggcttcac gcagggtttc aaccagttgc agtacctgag
     1321 tcgcagccag gccgaacttc gatttttccc cgccggagga ctgccattta cccgagccct
     1381 gcgaagccag acgtgcacgc acgcccagac gaggaacgac attcagacgt tctgcttcat
     1441 ccagcacaat ggcgatttct gacatcttct caatgaccag atagaccttg tgccccatct
     1501 tctcgccaat taatgccagg cggatatatt cgcggtcttt ataaccgttg cagacgatga
     1561 cgctacgagt catgccagca tgtgccagca ctgccatcaa ctcggctttg gaaccggctt
     1621 ccagacccag cggttcaccc gaatgaatca gggactcgat cacgcggcgg tgctggttaa
     1681 ctttgatcgg ataaacaagg aagtaatcgc cgttatagcc gtaggattcc ctcgcacgtt
     1741 tgaacgcggc gttaatggaa cgcaaacggt gctgcaggat ctgtgggaaa cagaacagtg
     1801 caggcagacg ctgaccctgt gcttcacgag ttttcactaa ctgcgcgaga tcgacgcgag
     1861 cttccgggac gtccgggtcc gggcacacgc tgatgtggcc cagctcgtta acgtcatagt
     1921 agttattgcc ccaccaggca atattgtaag tacgcagcat cttgctggct tcctgggagc
     1981 tcattgcaac ctcctgcatg gagcgtagta caccgtgttc gcccgctgac gaaggcaaac
     2041 ccatagacat gtcgtcagac atagcgaacc tcaaattatt ttattaagtg taaaacagtt
     2101 aacgactatc gcagcctgga actgcgataa caacccataa gcacatggat tttccagcag
     2161 tgtatgctga cgctccgact gcgcaaccgg tttctttttc ataacattat taagcacata
     2221 accgaacgta agtgtgacag ttcggcgaaa ccacgagaaa actcttgttt ttacaagagc
     2281 gcccttgttc agtcctcagt aactgtaacc agctcttgaa tcctgagaag cgccgagatg
     2341 ggtataacat cggcaggtat gcaaagcaga gatgcagagt gcggggaacg aatcttcacc
     2401 agaacggtga gacaggttaa gcagcagaca acggttcatt atttcgtatc acctccacgg
     2461 ccgcctgtta agacgaaccc acaagccaaa actctgattt caacccggct ggaagtggca
     2521 acacgaaaga aacgtcgtgt gctttttatt taagccgcgc gccgcgtttt ataccccaca
     2581 atggcagaaa attgcaaaag ataaatacgc agaatgccgg cattgtcagg aaaaatttcc
     2641 agccacgttt ttaacagaat gagacacgat tcaaaaaaaa gtggaaatcg ggtgaagaat
     2701 tgacctaaaa tagccatcca gatgttaatc catccatacc gattaacact cagactgcca
     2761 gtgtttttaa cctgcagagt cgtggtagga tccgctacca cagaaaatcc acacaacagt
     2821 ttgagctaac caaattctct ttaggtgata ttaaatatgg caaaacacct ttttacgtcc
     2881 gagtccgtct ctgaagggca tcctgacaaa attgctgacc aaatctctga tgccgtttta
     2941 gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta cgtaaaaacc
     3001 ggcatggttt tagttggcgg cgaaatcacc accagcgcct gggtagacat cgaagagatc
     3061 acccgtaaca ccgttcgcga aattggctat gtgcattccg acatgggctt tgacgctaac
     3121 tcctgtgcgg ttctgagcgc tatcggcaaa cagtctcctg acatcaacca gggcgttgac
     3181 cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg ctacgcaact
     3241 aatgaaaccg acgtgctgat gccagcacct atcacctatg cacaccgtct ggtacagcgt
     3301 caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc gcccggacgc gaaaagccag
     3361 gtgactttcc agtatgacga cggcaaaatc gttggtattg atgctgtcgt gctttccact
     3421 cagcactctg aagagatcga ccagaaatcg ctgcaagaag cggtaatgga agagatcatc
     3481 aagccaattc tgcccgctga atggctgact tctgccacca aattcttcat caacccgacc
     3541 ggtcgtttcg ttatcggtgg cccaatgggt gactgcggtc tgactggtcg taaaattatc
     3601 gttgatacct acggcggcat ggcgcgtcac ggtggcggtg cattctctgg taaagatcca
     3661 tcaaaagtgg accgttccgc agcctacgca gcacgttatg tcgcgaaaaa catcgttgct
     3721 gctggcctgg ccgatcgttg tgaaattcag gtttcctacg caatcggcgt ggctgaaccg
     3781 acctccatca tggtagaaac tttcggtact gagaaagtgc cttctgaaca actgactctg
     3841 ctggtacgtg agttcttcga cctgcgccca tacggtctga ttcagatgct ggatctgctg
     3901 cacccgatct acaaagaaac cgcagcatac ggtcactttg gtcgtgaaca tttcccgtgg
     3961 gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg gtctgaagta atctttcttc
     4021 acctgcgttc aaaggccagc ctcgcgctgg cctttttctt ttacctcacc gcggtggata
     4081 ggcgttcacg ccgcatccgg caaaaaaact gcccgcacaa taacatcatt cttcctgatc
     4141 acgtttcacg gcaaattacc atcacaactg aaaccgatta caccaaccac accagcaaaa
     4201 tatttgtaat attttcatat tgttattcag ctttcacagt tgttacattt cttttcagta
     4261 aagtcttaat tgcagatgtc agcgtttaat ctatgatgat ataactcaat tattttcatg
     4321 cacttaaatc ataactaaga taaatgttag tgtaagcgat tacactaatg tgatttgctt
     4381 cacatctttt tacgtcgtac tcacctatct taattcacaa taaaaaataa ccatattgga
     4441 gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac aaggcaatga cgtttttcgt
     4501 ctgcttcctt gccgctctgg cgggattact ctttggcctg gatatcggtg taattgctgg
     4561 cgcactgccg tttattgcag atgaattcca gataacttcg cacacgcaag aatgggtcgt
     4621 aagctccatg atgttcggtg cggcagtcgg tgcggtgggc agcggctggc tctcctttaa
     4681 actcgggcgc aaaaagagcc tgatgatcgg cgcaatcctg tttgttgccg gttcactgtt
     4741 ctctgcggct gcgccaaacg ttgaagtact gattctttcg cgcgttctgc tggggctggc
     4801 ggtgggtgtg gcctcttata ccgcaccact gtacctgtca gaaattgcgc cggaaaaaat
     4861 tcgcggcagt atgatctcga tgtatcagtt gatgatcact atcgggatcc tcggtgctta
     4921 tctttctgat accgccttca gctacaccgg tgcatggcgc tggatgctgg gtgtgattat
     4981 catcccggca attttgctgc tgattggtgt cttcttcctg ccagacagcc cacgttggtt
     5041 tgccgccaaa cgccgttttg ttgatgccga acgcgtgctg ctacgcctgc gtgacaccag
     5101 cgcggaagcg aaacgcgaac tggatgaaat ccgtgaaagt ttgcaggtta aacagagtgg
     5161 ctgggcgctg tttaaagaga atagcaactt ccgccgtgcg gtgttccttg gcgtactgtt
     5221 acaggtaatg cagcaattca ccgggatgaa cgtcatcatg tattacgcgc cgaaaatctt
     5281 cgaactggcg ggttatacca acaccaccga gcaaatgtgg gggacagtga ttgtcggcct
     5341 gaccaacgta cttgccacct ttatcgcaat cggccttgtt gaccgctggg gacgtaaacc
     5401 aacgctaacg ctgggcttcc tggtgatggc tgctggcatg ggcgtactcg gtacaatgat
     5461 gcatatcggt atccactctc cgtcggcgca gtatttcgcc atcgccatgc tgctgatgtt
     5521 tattgtcggt tttgccatga gtgccggtcc gctgatttgg gtactgtgct ccgaaattca
     5581 gccgctgaaa ggccgcgatt ttggcatcac ctgctccacc gccaccaact ggattgccaa
     5641 catgatcgtt ggcgcaacgt tcctgaccat gctcaacacg ctgggtaacg ccaacacctt
     5701 ctgggtgtat gcggctctga acgtactgtt tatcctgctg acattatggc tggtaccgga
     5761 aaccaaacac gtttcgctgg aacatattga acgtaatctg atgaaaggtc gtaaactgcg
     5821 cgaaatcggc gctcacgatt aatctcctca ggcttcctcc cgtcgcggag gaagccgcct
     5881 cttgcagtca tcttttcttc gctctatcct ctgccgctat gaaaacatcc cgtctcccta
     5941 tcgccatcca acaggccgtt atgcgccgcc tgcgggaaaa actcgcccag gccaacctga
     6001 agctagggcg taactacccg gagccaaaac tctcttacac ccagcgcgga acctccgccg
     6061 gaacagcctg gctggaaagc tatgaaatcc gcctcaatcc cgttttgctg ttggaaaaca
     6121 gtgaagcttt tattgaagaa gtggtaccgc acgaactggc acatttgctg gtatggaaac
     6181 atttcggccg cgtagcgcca catggcaaag agtggaagtg gatgatggaa agcgtgctgg
     6241 gcgttcccgc ccgtcgtacg catcagttcg aactgcaatc cgtgcgtcgc aacaccttcc
     6301 cctaccgctg taagtgccag gaacatcagc ttaccgtacg ccgccataat cgcgtagttc
     6361 gtggcgaggc catctatcgc tgtgttcact gcggtgaaca gctggttgcg aaataaccac
     6421 ctgaactatc aggaactttc ctgatctggc tgattgcata ccaaaacaac tttcgctacg
     6481 ttgctggctc gttttaacac ggagtaagtg atgtaccgtt atttgtctat tgctgcggtg
     6541 gtactgagcg cagcattttc cggcccgacg ttggccgaag gtatcaatag tttttctcag
     6601 gcgaaagccg cggcggtaaa agtccacgct gatgcgcccg gtacgtttta ttgcggatgt
     6661 aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc aatcgtgcgg ctatcaggtg
     6721 cgcaaaaatg aaaaccgcgc cagccgcgta gagtgggaac acgtcgttcc cgcctggcaa
     6781 ttcggtcacc agcgccagtg ctggcaggac ggtggacgta aaaactgcgc taaagatccg
     6841 gtctatcgca agatggaaag cgatatgcat aacctgcagc cgtcagtcgg cgaggtgaat
     6901 ggcgatcgcg gcaattttat gtacagccag tggaatggcg gcgaaggcca gtacggtcaa
     6961 tgcgccatga aggtcgattt caaagaaaaa gctgccgaac caccagcgcg tgcacgcggt
     7021 gccattgcgc gcacctactt ctatatgcgc gaccaataca acctgacaca ctctcgccag
     7081 caaacgcagc tgttcaacgc atggaacaag atgtatccgg ttaccgactg ggagtgcgag
     7141 cgcgatgaac gcatcgcgaa ggtgcagggc aatcataacc cgtatgtgca acgcgcttgc
     7201 caggcgcgaa agagctaacc tacactagcg ggattctttt tgttaacccc taccccacgc
     7261 gtacaaccgc gtggggagac gacgcggatt tttaactatg cgtatccccc gcatttatca
     7321 tcctgaacca ctgaccagcc attctcacat cgcgctttgc gaagatgccg ccaaccatat
     7381 cgggcgcgta ctgcgcatgg ggccggggca ggcgttgcaa ttgtttgacg gtagcaacca
     7441 ggtctttgac gccgaaatta ccagcgccag caaaaaaagc gtggaagtga aggtgctgga
     7501 aggccagatc gacgatcgcg aatctccgct gcatattcac ctcggtcagg tgatgtcgcg
     7561 tggtgaaaaa atggaattta ctatccagaa atcgatcgaa ctcggtgtaa gcctcattac
     7621 gccacttttt tctgagcgct gcggcgttaa actggatagt gaacgtctga acaagaagct
     7681 tcagcagtgg cagaagattg caattgctgc ctgtgagcag tgtggtcgta accgggtgcc
     7741 ggaaatccgt ccagcgatgg atctggaagc ttggtgtgca gagcaggatg aaggactgaa
     7801 actgaatctt cacccgcgcg ccagtaacag catcaatacg ttgcctttac cggttgaacg
     7861 cgtccgcctg ctgattggcc cggaaggcgg tttatcggca gatgaaattg ccatgactgc
     7921 ccgctatcaa tttactgata tcctgttggg acctcgcgtt ttgcgtacag agacaactgc
     7981 gctcaccgcc attaccgcgc tacaagtacg atttggcgat ttgggctaac ggagaagaat
     8041 aatgatcaag ctcggcatcg tgatggaccc catcgcaaac atcaacatca agaaagattc
     8101 cagtttcgct atgttgctgg aagcacagcg tcgtggttac gaacttcact atatggagat
     8161 gggcgatctg tatctgatca atggcgaagc ccgcgcccat acccgcacgc tgaacgtgaa
     8221 gcagaactac gaagagtggt tttcgttcgt cggtgaacag gatctgccgc tggccgatct
     8281 cgatgtgatc ctgatgcgta aagacccgcc gtttgatacc gagtttatct acgcgaccta
     8341 tattctggaa cgtgccgaag agaaagggac gctgatcgtt aacaagccgc agagcctgcg
     8401 cgactgtaac gagaaactgt ttaccgcctg gttctctgac ttaacgccag aaacgctggt
     8461 tacgcgcaat aaagcgcagc taaaagcgtt ctgggagaaa cacagcgaca tcattcttaa
     8521 gccgctggac ggtatgggcg gcgcgtcgat tttccgcgtg aaagaaggcg atccaaacct
     8581 cggcgtgatt gccgaaaccc tgactgagca tggcactcgc tactgcatgg cgcaaaatta
     8641 cctgccagcc attaaagatg gcgacaaacg cgtgctggtg gtggatggcg agccggtacc
     8701 gtactgcctg gcgcgtattc cgcagggggg cgaaacccgt ggcaatctgg ctgccggtgg
     8761 tcgcggtgaa cctcgtccgc tgacggaaag tgactggaaa atcgcccgtc agatcgggcc
     8821 gacgctgaaa gaaaaagggc tgatttttgt tggtctggat atcatcggcg accgtctgac
     8881 tgaaattaac gtcaccagcc caacctgtat tcgtgagatt gaagcagagt ttccggtgtc
     8941 gatcaccgga atgttaatgg atgccatcga agcacgttta cagcagcagt aacccacctt
     9001 agcgagaagg atctcgttga gactctgagt gacagcgccc ttctttccac gcatactggg
     9061 cgctgttgct tttttgaacc aggaaacaga acctctgaca atgaatttac agcatcactt
     9121 tcttattgcc atgcctgctc tccaggatcc gattttccgt cgttccgtgg tctacatttg
     9181 cgaacataat accaatggtg caatggggat catcgtcaac aagccgctgg aaaatctcaa
     9241 aattgaaggg attctggaaa agctgaagat ctcgccggag ccgcgtgatg aatcaatccg
     9301 tctggataaa ccggttatgc tcggcggccc actggctgaa gatcgcgggt ttattttgca
     9361 tactccgccc tccaattttg cttccagcat tcgcatttca gacaacacgg tgatgaccac
     9421 ttcccgcgat gtgctggaaa cgctcggcac cgataaacaa ccgtctgacg tattggtggc
     9481 tctgggctat gcctcctggg aaaaaggtca actggaacaa gaaattctcg ataacgcgtg
     9541 gctaacggcc ccggcggatc tgaatattct gttcaaaacg ccgattgccg accgctggcg
     9601 cgaggcggca aaactgattg gtgtggatat tctcaccatg cctggtgtgg caggacacgc
     9661 ctgatgagtg gaaccttact cgccttcgac ttcggcacca aaagcattgg cgtagcggtc
     9721 ggccaacgca ttaccggcac cgctcgccct ttgcctgcaa ttaaagcgca ggacggtacg
     9781 ccggactgga acctcatcga acgtttactg aaagagtggc agcctgacga aatcatcgtc
     9841 ggtttgccgc tgaatatgga cggcaccgaa cagccgttaa ccgccagagc gcgcaaattt
     9901 gccaaccgta ttcatggtcg tttcggtgtt gaagtaaagc tccatgacga gcgtcttagc
     9961 actgtggaag cccgttccgg tctttttgaa cagggcggct atcgagcgct caacaaaggc
    10021 aaagttgact ctgcctctgc ggttattatt ctcgaaagct atttcgagca gggatattaa
    10081 ggcgatctaa aagcctggcg gatagtcgtt accgccatct tgccggatgc ggcgtgaacg
    10141 ccttatccag cacacatctg gcagcggctg taggtctgat aagacgcgtt agcgtcgcat
    10201 cagacattta ttgcctttat aggccctata aacttgcgca tcgggcatgg caacgtcaca
    10261 aacgcccttc ccccacccgc tgctgataac tctgctgaaa cgttaacatc cccacctgct
    10321 acccggtttg aataacatgc ggtaactggt gggttttccc ttcgcgaatc aaattcccca
    10381 ccgcgggagt gttaatcagt aattcaaaca gcgccacgcg tccttcctgt ttatccactt
    10441 ccagcttttg tgacagcact gcccgtaaac tacccgcaag ttgattacgt acgggatctt
    10501 tttcctgcgc cggaaatgaa tccaccagtc gctcaactgc ctgcgcggca ccacgtgtat
    10561 gtaaggttgc cagcaccaga tgcccggttt ctgccgccgt cagcgccaga cggattgtct
    10621 cactgtcacg tagctctccg agcaaaatca catcaggatc ttcccgcaat gcagcccgca
    10681 accccgatgc gaacgtcata cagtgcaaac caatttcccg ctgctggatc aaacatcgct
    10741 ggctggcata gagatattcc acaggatctt ccagcgtcag aatatgcgca tcggcatgtt
    10801 gattaagata gccaaccatc gccgccagcg tggtagattt gccactcccc gtagcccccg
    10861 tcaccagaat caggccattc tcgctcttga gtaattccgg caataccggt ggcgcaccaa
    10921 gctgttcgag ctgtggacag tgcgaaggta acaaccgtaa tgccagagaa ataccttgcc
    10981 gttgcgcgaa cgcactgcca cgcaaccgct ggttttccgc cagcgacacg gcaaaatcca
    11041 gctggccatt ttccagcaat attgcccgct gatcgtcatc cagccactcc cgcagtagct
    11101 cttcgacgtc cggcgcatca aacggcgcag cttccattct gccacgaatg cgccatcgtg
    11161 cgggccaggc gctgcacagg tgtagatccg agacgttatg ctttacacta agggccacaa
    11221 tttcttccat attcatacta agatcctcgg aaaatgaacg atattgcgca taacctggca
    11281 caggtccggg acaaaatctc agcggctgca acgcgttgcg gccgttctcc agaagaaatt
    11341 acgctgcttg cagtcagtaa aacaaaacct gcgagcgcca tcgcagaagc cattgatgcc
    11401 gggcagcgtc aatttggtga aaactacgtt caggaagggg tagataaaat tcgccacttt
    11461 caggaactgg gcgtaacagg attagaatgg cattttattg gcccgttgca gtctaataaa
    11521 agccgcctag tggcagagca tttcgactgg tgtcatacca tcgaccgttt gcgcattgct
    11581 acccgcctca acgatcagcg ccctgcagaa cttccccctc ttaacgtact gattcaaatt
    11641 aatattagtg atgaaaacag taagtccggg attcaactgg cagaactgga cgagctggca
    11701 gcggcggtcg ctgaactacc gcgtttacgt ctgcgcgggc tgatggcaat ccctgcgcct
    11761 gagtcagaat atgtaaggca gtttgaagtt gcacgccaaa tggctgtagc atttgccgga
    11821 ctgaaaacgc gctacccgca tatcgacacg ctctctctgg gaatgtcgga cgatatggaa
    11881 gccgccattg cggcaggtag cacgatggtt cgtatcggca ctgcaatttt tggtgcgcgt
    11941 gattactcta aaaaataagg aattaaagga acgccatgaa tacgttgact ttcctgcttt
    12001 caacggtcat tgagctgtat accatggtgc tgttattacg catctggatg cagtgggctc
    12061 attgtgattt ttacaacccc ttctcacagt ttgtagtgaa ggtagcgcag ccaattatcg
    12121 ggccactgcg ccgcgttatt ccggcaatgg gtcccattga cagcgcctcg ctgctggttg
    12181 cctatattct cagttttatc aaagccattg tgctgtttga agtggtgacc ttcctgccaa
    12241 tcatctggat tgccggttta ctgattctgc tgaaaaccat cggcttgctg attttctggg
    12301 tcttgctggt gatggcgatt atgagctggg taagccaggg tcgtagcccg attgaatacg
    12361 tgctgattca gctggccgat ccgctgctgc gcccgattcg ccgcttgcta ccggcaatgg
    12421 gtgggattga tttctcgccg atgatcctcg ttctgctgct gtatgtcatc aatatgggtg
    12481 tcgcagaagt attacagtca accggaaaca tgctgctgcc ggggctgtgg atggcgttat
    12541 gagtgccgta acagttaatg atgacggtct ggttttacgg ctctatattc agccgaaagc
    12601 cagccgtgat tctattgtcg gtttacatgg cgacgaagtt aaagtcgcca ttaccgcgcc
    12661 gccggttgac ggccaggcca acagtcatct ggtgaagttt ctcggtaagc aattccgggt
    12721 tgccaaaagc caggtggtga ttgaaaaagg cgaacttggc cgccacaaac aaattaaaat
    12781 cattaatccg caacaaatcc cgccagaaat cgcggcgtta attaattagg tatcctatgc
    12841 aaaaagttgt cctcgcaacc ggcaatgccg gtaaagtgcg tgagctggcg tcgctgctta
    12901 gcgactttgg tcttgatatc gtggcccaaa cagacctcgg cgttgattcc gctgaagaaa
    12961 ccggcctaac ctttatcgaa aacgcgattc tgaaagcgcg ccatgcggca aaagtgaccg
    13021 gtttaccggc aattgccgac gactctggtc tggcggtaga tgcgctgggt ggcgcgccgg
    13081 gtatttactc cgcgcgttat tccggtgaag acgcgaccga tcaaaagaat ctgcaaaaac
    13141 tgttggaaac actgaaagac gtaccggacg accaacgtca ggcgcgtttc cactgcgtgc
    13201 tggtatatct gcgtcacgcg gaagatccga ctccgctggt atgccacggc agctggccgg
    13261 gcgtgattac tcgcgaacca gcgggcactg gtggctttgg ttatgatcca atcttcttcg
    13321 taccttccga agggaaaacc gccgccgaac tgacccgcga agaaaagagc gccatttccc
    13381 accgtggtca ggcgttgaaa ctgctgctgg acgctttacg taatggttaa attaccgccg
    13441 ctgagtctct acattcacat cccgtggtgc gtgcagaaat gcccgtactg cgatttcaac
    13501 tctcacgcgt tgaaaggaga agtgccgcac gacgattatg ttcagcatct gctttgcgat
    13561 ctggacaatg atgtggctta cgctcagggc cgtgaagtaa agacaatctt tattggcggt
    13621 ggtacgccga gcctgctttc cggcccggcg atgcaaacgc tgctggacgg cgtgcgtgcg
    13681 cgtttgccgc tggcagcgga tgcagaaatt actatggaag cgaacccagg cacggtagaa
    13741 gcggatcgct ttgtcgatta tcagcgtgct ggtgtaaacc gcatctctat tggtgtgcag
    13801 agttttagcg aagaaaagct gaaacgactt gggcgtattc atggcccgca agaagcgaaa
    13861 cgagcggcaa agctggcgag cggtttaggg ttacgtagct ttaaccttga tttgatgcat
    13921 ggactaccgg atcaatcact ggaagaggcg cttggcgatc tacgccaggc cattgaactg
    13981 aatccgccgc atctttcctg gtatcaactg accatcgaac ctaatacgct gtttggctcg
    14041 cgaccgccgg tgctgccgga cgatgacgcg ctgtgggata ttttcgaaca ggggcatcag
    14101 ttattaaccg cagcgggtta tcagcaatat gaaacttccg cttacgccaa acccggttat
    14161 cagtgccagc acaatctcaa ctactggcgc tttggtgact acatcggtat tggctgcggc
    14221 gcgcacggca aagtgacctt cccggatggg cgcattctgc gtaccactaa aacgcgtcat
    14281 cctcgtggtt ttatgcaagg aaggtatctg gaaagccagc gtgatgtcga agccgcagat
    14341 aagccgtttg agttctttat gaatcgcttc cgtctgctgg aggccgcgcc gcgcgtggag
    14401 tttattgcgt ataccgggct ttgcgaagat gtgattcgcc cacagttaga cgaggcgatt
    14461 gcccagggtt atctcaccga atgtgcggat tactggcaga taacggaaca tggtaaatta
    14521 ttcttaaact cacttcttga attattcctc gctgagtaat cattaatgcg cctgcgggcg
    14581 cattaatcac ttcttttatc cccccgttaa ttaacctcgg cagaaatata tttctt
//
LOCUS       DADLSB010000100        14605 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.100, whole genome shotgun sequence.
ACCESSION   DADLSB010000100 DADLSB010000000
VERSION     DADLSB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.100"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(175..1368)
                     /locus_tag="JN331_002820"
     CDS             complement(175..1368)
                     /locus_tag="JN331_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7260925.1"
                     /translation="MSPGIEDTPQKPQSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1504..3228
                     /gene="iucA"
                     /locus_tag="JN331_002821"
     CDS             1504..3228
                     /gene="iucA"
                     /locus_tag="JN331_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY7260926.1"
                     /translation="MPLPSENPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3229..4176
                     /gene="iucB"
                     /locus_tag="JN331_002822"
     CDS             3229..4176
                     /gene="iucB"
                     /locus_tag="JN331_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY7260927.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4176..5918
                     /gene="iucC"
                     /locus_tag="JN331_002823"
     CDS             4176..5918
                     /gene="iucC"
                     /locus_tag="JN331_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY7260928.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            5915..7252
                     /gene="iucD"
                     /locus_tag="JN331_002824"
     CDS             5915..7252
                     /gene="iucD"
                     /locus_tag="JN331_002824"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY7260929.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7258..9453
                     /gene="iutA"
                     /locus_tag="JN331_002825"
     CDS             7258..9453
                     /gene="iutA"
                     /locus_tag="JN331_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY7260930.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10413..14300
                     /gene="sat"
                     /locus_tag="JN331_002826"
     CDS             10413..14300
                     /gene="sat"
                     /locus_tag="JN331_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY7260931.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTANHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         3894 a         3501 c         3640 g         3570 t
ORIGIN      
        1 gatcataggt tgttgcacga aagaatcatg gagattacac cgcagcaact ggttgaattc
       61 actatacctg atgggcgggg gcagcaagcc agaacactgc ctgacatgct ttcatcagac
      121 gcaggtcctc tctcatatcc tctgatttac tccagtaagg tgatgatcat atctttattt
      181 gatagtacgg taatgccaga taaatacaga cacaatggta aataatatag ttgcaaataa
      241 tgctgccata atcagcgcga ttgcgacacc tgttttcgat accagcggca ccagcaaagc
      301 gcacagtcca tagccaagtg tgtgactggt agcgagccag cccgcgcctg cgccatcagc
      361 cagcttatca ttcagtagaa gttgataagc gggtgttgcc agagctgctc caaatgacag
      421 cactgcacag cccgggtaaa acaaccatag ctgttcggag agcatgatag ccagaccacc
      481 actcatcaac actccggcac tcaggagcag ggccaccgga gtcaggcgct gcggacggag
      541 taccccgaac tgcgcgataa gcgcagctac tgcggacaga cccaacagcc aggccacctg
      601 ttggctaatg gcggtggtat ccgtagcgaa ctggcgagta agggcaggcg aaagtccaag
      661 ctgcatcatg ctcaccgccg cagccagtaa tattgcgcaa agcagataag gcagacaatc
      721 tcgcttcaga ctgacgctct tgcactccgg tgtgggctgt ggtggtgtgc cgggcaaccg
      781 cagcagcatc agcagcgcca gcactggggc tgccatcagc agccccagtg gcgccagagg
      841 atgaatggcc agcattgctg ccgcgcacag cggcccgaat agcctgccgc aactcaggcc
      901 ggagctgatg gttgccagag cggccatgcg attcccttct ccagctctct gcaatgccca
      961 gacctgacag gctggcacca ttgccgacac ggtcagcccg taggcgatcc gggcgatgat
     1021 taatattccc agtcccaccg ctgttgttat ggcgctggta gccatcagca cgcttcccag
     1081 ccctagcagg gtaaagctta tgccatatcc tcccagagcc cacaacacca caggtttact
     1141 gccagtacgg gaaatttgct ttccccacca cggggacgat ggcagaaaca gcattgagcc
     1201 aagcatcagc agggcagccc atacagacag actcagattt gtctgtatga ccagaacagg
     1261 gagcacaacc agcagaccgt tctgtccgat accgagaagc ccggcactga acgcaagtgg
     1321 ccagcaggac tgtggttttt ggggcgtatc ttcgatccct ggtgacatta taatgtttca
     1381 actccatgta ttaattatgt ttatttgtaa aattaattta tctgacaata acattcattg
     1441 ttgataatga gaatcattat tgacataatt gttattattt tactgtgtgg gagctgtttg
     1501 actatgcccc tgccctctga aaacccagcc acagatgtgg ctgcgcagtg cttcctgaat
     1561 gcactgattc gtgaaaccac agactggaaa ctgacagaat acccgccaga cgaattgctc
     1621 atcccgctgg atgagcagaa atcgctccat ttcagagtgg cttatttctc cccaacccag
     1681 catcaccgct ttgcatttcc tgcacgtctg gtcacggcat caggcagtta tcctgtcgac
     1741 tttaccactc tctcccggct tattattgat aagctacgcc atcaactgtt tctgcccgtt
     1801 cccctctgcg aaactttcca ccagcgcgtg ctggaaagcc atgtccatac gcaacaggca
     1861 attgatgccc gtcatgactg ggccgctctg cgtgaaaaag cgttgaattt tggcgaggct
     1921 gagcaggcgc tgctgacagg acacgctttc caccctgcgc ctaagtctca tgaaccgttt
     1981 aaccggcggg aggctgaacg ctacctgcct gatatggcac cccacttccc actgcggtgg
     2041 ttttcggtgg ataaaacgca aatcgccggt gaaagtttgc atctcaacct tcaacagcgg
     2101 ctgacgcgat ttgccgcaga gaatgcgcct cagttactca acgaattaag tgacaaccaa
     2161 tggctgttcc cgttgcaccc gtggcagggg gaatatcttt tgcagcaggg gtggtgccag
     2221 gcacttgttg ctaaagggct gattaaagac ttaggtgagg ccggcacgtc gtggctgccg
     2281 accacctctt cccgttccct ctactgtgcc accagccgcg atatgatcaa gttctccctg
     2341 agcgtacggc tgaccaactc catccgtact ctgtccgtga aagaagtgaa gcgtgggatg
     2401 cgcctggcac gcctggctca aaccgacggc tggcagatgc tacaggtccg cttcccgact
     2461 ttccgggtaa tgcaggagga tggctgggcc gggctgctcg atcttaacgg caacatcatg
     2521 caggaaagtc tgtttgccct gcgtgaaaat ctgctggtgg atcagccgaa aagccagact
     2581 aacgtactgg tctccctgac tcaggccgca cctgatggcg gtgattcgct gctggtttcg
     2641 gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc aacaggccgc ccatgcatgg
     2701 gtcgatgcat actgtcagca agtgctaaag ccgctgttta cggctgaagc ggattacggc
     2761 ctggtgctgc tggcgcatca gcaaaatatt cttgtccaga tgcttgggga tctgccggtc
     2821 ggatttattt accgtgactg tcagggcagc gcttttatgc ctcacgcgac agactggctc
     2881 gattctattg gcgaggcgca ggcggaaaat attttcaccc atgagcagtt gctgcgctat
     2941 ttcccttatt acctgctggt taactccact tttgctgtga ccgctgcgct gggggctgcc
     3001 gggctggaca gcgaatcgaa tctgatggct cgtgtacgag catcgctggc tgaagtgcgt
     3061 gatcaggtga ctcataaaac ctgtctcaac tacgtgctgg aaagtccgta ctggaacgta
     3121 aaaggtaact ttttctgtta tctgaacgat cataacgaga ataccattgt tgacccttcg
     3181 gtgatctatt tcgatttcgc taacccgctg caggctcagg aggtctgaat gtctgaggca
     3241 aacattattc acagcagata tggactgcgc tgtgaaaaac tcgacaagcc cctgaatctt
     3301 ggctggggac tggacaatag cgcggtgttg cactgtcccg gggagctgcc gacagggtgg
     3361 ctgtgcgatg cgctggatca gatatttatc gccgcaccac aactgtcagc agtggctctc
     3421 ccctgggccg aatggcgtga ggagccacag gcgctgacgc tttttgggca ggtaaaaagc
     3481 gacattatcc atcgtactgc cttctggcag ttaccgttat ggttgagttc accggcaaac
     3541 cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga tttatttccc gcagcgccca
     3601 ccccgtccac agggtgaggt ttaccgccgt tatgatccac gtattcgcag gatgctgagt
     3661 ttccgcattg ccgatcccgt ttctgatgca gaacgtttca cccgctggat gaatgatccg
     3721 cgcgttgagt atttctggga gcaaagtggc tcactggagg tacagaccgc ctatctggag
     3781 cgccagctca ccggtaaaca tgcattcccg ctgattgggt gcttcgacga tcggccattc
     3841 agctatttcg aaatctactg ggcggcggaa gaccgcattg gtcgtcacta ttcgtggcag
     3901 ccctttgacc gtggcctgca tctgctggtt ggtgaacagc aatggcgcgg ggcccactat
     3961 gtgcaaagct ggctgcgcgg gctgacacat tacctgctgc tggatgagcc ccgtacgcag
     4021 cgcacagtac tggagccacg caccgataac cagcgcctgt tccgccatct tgagcctgcg
     4081 ggataccgga caattaaaga gttcgacttc ccacataagc gctcgcgcat ggtgatggcg
     4141 gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa tcacaaggac tgggattttg
     4201 ttaaccgtcg gctggtggca aaaatgttgt ctgagatgga gtatgagcag gttttccacg
     4261 cggaatctca gggcgatgac cactactgca ttaacctgcc gggagcacaa tggcgcttca
     4321 tcgctgaacg tggtatctgg ggctggctgt ggattgatgc ccaaacgttg cgctgcacgg
     4381 acgaaccagt actggcgcag acgctgctga tgcagctaaa gccggtgctg tcaatgagtg
     4441 atgccactgt tgcagagcat atgcaggatt tgtatgccac gctactgggc gacctgcaac
     4501 tgctgaaggc ccgtcgcgga ctgagtgcca gtgacctgat taatcttgat gccgatcgtc
     4561 tgcaatgcct gctgagcggt catcctaaat tcgtttttaa taaaggtcgc cgtggctggg
     4621 gcaaagaggc gctggaacga tatgcgccag agtataccaa caccttcaga ctgcactggc
     4681 tggcggtaaa acgtgaacat atgatctggc gctgcgataa cgatctggat attcagcagt
     4741 tgttgactgc cgcaatggat ccgcaggagt ttacacgctt cagtcaggtc tggcaggaaa
     4801 acgggctgga tcacaactgg ctgccgctgc cggtacatcc gtggcagtgg cagcaaaaaa
     4861 tcgccacaga cttcatcgct gattttgccg aaggcaggat ggtgtctctc ggcgagtttg
     4921 gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac caacgccagc cgccggggag
     4981 ggctggatat caagctgccg ctgaccatct acaatacctc atgctaccgg gggatccctg
     5041 gcagatatat cgctgccgga ccactagctt cacgctggct gcaacaggtt tttgcgaccg
     5101 acgccaccct ggtgcaaagc ggcgcagtga tccttggtga accggctgca ggctatgtgt
     5161 cccatgaagg ctatgccgcg cttgcccggg ctccctatcg ctaccaggaa atgcttggtg
     5221 ttatctggcg ggagaatccg tgccgctggc tgaaaccgga tgaaagtcct gttctgatgg
     5281 caacactgat ggagtgcgac gaaaacaatc agccgctggc aggcgcatat atcgaccgct
     5341 ccggactgga cgctgaaacc tggcttacgc aattgttccg ggtggttgtg gttccgctgt
     5401 atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca cggacaaaat ataacgctcg
     5461 ccatgaaaga gggggtacca cagcgtgttc tgctgaaaga cttccagggc gatatgcggc
     5521 tggtgaaaga agcgtttccc gaaatggact ctttgcctca ggaggtgcgt gatgtcacat
     5581 cccgtctgag tgcggactac ttaatccatg atttgcagac gggtcacttc gtgacagtac
     5641 tgcgttttat ttcgccactg atggttcgtc ttggcgtacc tgaaaggcga ttttatcaac
     5701 tgctggcagc agtgttgagt gattacatga acaaacatcc acaaatggca gagcgttttg
     5761 cgcttttctc actcttcagg ccacaaatca ttcgcgtggt gctgaacccg gtaaaactga
     5821 cctggccgga cctggatggc ggcagtcgca tgctgccgaa ttaccttgag aatctgcaaa
     5881 atccgctgtg gctggtaact caggaatatg aatcatgaaa aaaagtgtcg attttattgg
     5941 tgtcgggaca gggccattta atctcagcat tgctgcattg tcacatcaga tcgaagaact
     6001 gaactgtctc ttctttgacg aacatcctca tttttcctgg catccgggta tgctggtacc
     6061 ggattgtcat atgcagaccg tctttctgaa agatctggtc agtgcagttg cacctacaaa
     6121 tccctacagc tttgttaact atctggtgaa gcacaaaaag ttctatcgct tccttacaag
     6181 cagactacgt acagtatccc gtgaagagtt ttctgactat ctccgctggg ctgctgaaga
     6241 tatgaataac ctgtatttca gtcataccgt tgaaaatatt gatttcgaca aaaaaagtcg
     6301 attgtttctg gttcagacca gtcggggaga atattttgcc cacaatatct gccttggtac
     6361 aggaaaacaa ccttatttac caccctgtgt gaagcatgtg acacaatcct gtttccatgc
     6421 cagtgaaatg aatcttcgtc ggccggacct gagtggaaaa cggataaccg tggttggtgg
     6481 aggacagagt ggtgcagacc tgttccttaa tgcattacgc ggggaatggg gagaagcggc
     6541 ggaaataaac tgggtctcac ggcgtaataa ttttaacgca ctggatgagg ctgcttttgc
     6601 tgatgagtat tttacacctg aatatatttc aggcttctcc ggactgaagg aagatattcg
     6661 ccatcagtta ctggatgagc agaaaatgac atcggatggc atcactgccg attctttact
     6721 gaccatttat cgtgagttgt accaccgttt tgaagttctg agaaaaccaa gaaatatccg
     6781 tctgctaccc agtcgctcgg taacaactct ggaaagtagt ggtccgggct ggaggttatt
     6841 gatggaacat catctggatc agggcagaga gagcctggaa agtgatgtgg tgattttcgc
     6901 cacaggttac cgttctgcat tgccacaaat acttccctca ctgatgccct tgatcaccat
     6961 gcacgataag aacaccttta aagtgcgtga tgacttcact ctggaatgga gtggcccgaa
     7021 agaaaataac atctttgcgg tcaacgccag catgcaaact catggcatcg ccgaacccca
     7081 gctcagcctg atggcctggc gatctgcacg tattcttaat cgcgtactgg ggcgtgattt
     7141 attcgatctc agtatgccgc ccgcactgat tcagtggcgc agcggcagcc ggaaaaaacc
     7201 gcagccggag gctgcttctt taactcacta tacagcaaat attcaggaat aacgatgatg
     7261 cgcaaaaagt atatgccccg ggctcttggt ccgctgcttc ttgtcgtgct gtcaccagct
     7321 gtcgcccagc aaaacgatga taatgagatc atagtgtctg ccagccgcag caatcgaact
     7381 gtagcggaga tggcgcaaac cacctgggtt atcgaaaatg ccgaactgga gcagcagatt
     7441 cagggcggta aagagctgaa agacgcactg gctcagttaa tccccggcct tgatgtcagc
     7501 agccagagtc gaaccaacta cggtatgaac atgcgtggcc gcccgctggt tgtcctgatt
     7561 gacggtgtgc gcctcaactc ttcacgttcc gacagccgac aactggactc tgtcgatcct
     7621 tttaatatcg accatattga agtgatctcc ggcgcgacgg ccctgtacgg tggcgggagt
     7681 accggagggt tgatcaacat cgtgaccaaa aaaggtcagc cggaaaccat gatggagttt
     7741 gaggctggca caaaaagtgg ctttaacagc agtaaagatc acgatgagcg cattgccggt
     7801 gctgtctccg gcggaaatga ccatatctcc ggacgtcttt ccgtggcata tcagaaattt
     7861 ggcggctggt ttgacggtaa cggcgatgcc accctgcttg ataacaccca gaccggcctg
     7921 cagcactcca atcggctgga catcatggga accggtacgc tgaacatcga tgaatcccgg
     7981 cagcttcaac tgataacgca gtactataaa agtcagggag acgacaatta cgggcttaat
     8041 ctcgggaaag gcttttccgc catcagcggg agcagcacac catacgtcag taaggggctg
     8101 aattctgacc gcattcccgg cactgagcgg catttgatca gcctgcagta ctctgacagt
     8161 gatttcctgg gacaggaact ggtcggtcag gtttactacc gcgatgagtc gttgcggtac
     8221 tacccgttcc cgacggtaaa tgcgaataaa caggcgacgg ctttctcctc gtcacagcag
     8281 gataccgacc agtacggcat gaaactgact ctgaacagcc aactgatgga cggctggcaa
     8341 atcacctggg ggctggatgc tgagcatgag cgctttacct ccaaccagat gttcttcgat
     8401 ctggctcagg caagtgcttc cggagggctg aacaaccata agatttacac caccgggcgc
     8461 tatccgtcat atgacatcac caatctggcg gccttcctgc aatccagcta tgacattaat
     8521 gatattttta ccgttagcgg tggcgtacgc tatcagtata ctgagaacag ggtagatgat
     8581 ttcatcgact acacgcagca acagaagatt gctgccggga aggcgatatc tgccgacgcc
     8641 attcctggtg gttcggtaga ttacgataac tttctgttca atgctggtct gctgatgcac
     8701 atcaccgaac gtcagcaggc atggttcaat ttttcccagg gggtggcatt gccggatccg
     8761 gggaaatatt atggtcgcgg catctatggt gcagcagtga acggccatct tcccctgaca
     8821 aagagcgtga acgtcagcga cagtaagctg gaaggcgtga aagtcgattc ttatgaactg
     8881 ggctggcgct ttatcggtga caacctgcgg actcaaatcg cggcatatta ctcgctttcc
     8941 aataagagcg tggaaaggaa taaagatctg accatcagtg tgaaggacga caggcgccgt
     9001 atttacggcg tggaaggtgc ggtggactac ttgatcccgg atactgactg gagtaccggt
     9061 gtgaacttca atgtgctgaa aaccgagtcg aaagtgaacg gtcaatggca aaaatatgac
     9121 gtgaaggaat caagtccatc gaaagcgaca gcttacatta actgggcgcc ggaaccgtgg
     9181 agtctgcgtg tacagagcac cacttctttc gacgtaagcg atgcagaggg taacgatatt
     9241 aatggttaca ctaccgtcga ttttatcagt agttggcagc ttccggtggg aacactcagc
     9301 ttcagcgttg agaacctctt cgaccgtgac tataccactg tctggggaca gcgtgcacct
     9361 ctgtactaca gcccgggtta cggccctgct tcactgtacg actacaaagg ccggggccga
     9421 acctttggtc tgaactactc agtgctgttc tgacggggat gcttttgcaa caagggtatg
     9481 ctgtaaacaa catggtcctt ggtggccatg ttgtttacag ccagtattgt ttacagccag
     9541 tataatatac agccttaaca agatattccg tctccggaat ggactgttca ccactgaata
     9601 acattatgtc tgttttatgg atctttgact gaaatttatc gcatatcttc atttcattgt
     9661 ccgtctgaaa actaacctta caagtaagcg tcgtctcagc accgtctggc agatcctgaa
     9721 attcctgaga ggatgatctg cgcccaatat cctgagccgg aactttgctg caatgaaggc
     9781 caacgaaaaa tgggggagta tgttatagag tacttgctac agggtaaaaa gaggtacctg
     9841 tcgctggttc tccgttcagt ttaaccggga gattttctcc tgcgcctgtc ataaagtccg
     9901 gtaatggaga ttgttaatac gatggtgcac gatgcattgt cagtgcccgg cccggaggat
     9961 gcaccgtagc tacatacgga tcagggttgg caatatcgaa tggcaggcta taggcaaagt
    10021 taaagcccca tggagtagca caaaatatgc cgcacaaagg aaacggtctg aataacgcag
    10081 tgatggagaa cttcttcggc acactgcaaa atcgcatcgt ggtcgatcgc taattcgtcg
    10141 tgaatcactg aacgatgttc catgcgaaca gatattcgat catatcgaaa tattaaacat
    10201 tacccctccc cacattgggc aaaaaaaaac aaaaaacgat caattaacga tcaattagta
    10261 ttgattcaca taagctctca tcattaatga gaacattgtc acatatttac aatatagaac
    10321 tgttactttt agcaagctgt tagtaaccaa ccgttactaa taattttaat tcttaatact
    10381 ttgttcattc aataaatgag ttgagagaat atatgaataa aatatactcc cttaaatata
    10441 gtgctgccac tggcggactc attgctgttt ctgaattagc gaaaagagtt tctggtaaaa
    10501 caaaccgaaa acttgtagca acaatgttgt ctctggctgt tgcaggtaca gtaaatgcag
    10561 caaatattga tatatcaaat gtatgggcga gagactatct tgatcttgca caaaataaag
    10621 gtattttcca gcccggagca acagacgtaa caatcacttt aaaaaacgga gataaattct
    10681 ctttccataa tctctcaatt ccggattttt ctggtgcagc agcgagtggc gcagctaccg
    10741 caataggagg ttcttatagt gttactgttg cacataacaa aaagaaccct caggccgcag
    10801 aaactcaggt ttacgctcag tcttcttaca aggttgttga cagaagaaat tccaatgatt
    10861 ttgagattca gaggttaaat aaatttgttg tggaaacagt aggtgccacc ccggcagaga
    10921 ccaaccctac aacatattct gatgcattag aacgctacgg tatagtcact tctgacggtt
    10981 caaaaaaaat cataggtttt cgtgctggct ctggaggaac atcatttatt aatggtgaat
    11041 ccaaaatctc aacaaattca gcatatagcc atgatctgtt aagtgctagt ctatttgagg
    11101 tcacccaatg ggactcatac ggcatgatga tttataaaaa tgataaaaca tttcgtaatc
    11161 ttgaaatatt cggagacagc ggctctggag catacttata tgataacaaa ctagaaaaat
    11221 gggtattagt cggaacaacc catggtattg ccagcgttaa tggtgaccaa ctgacatgga
    11281 taacaaaata caatgataaa ctggttagtg agttaaaaga tacctatagt cataaaataa
    11341 atctgaatgg caataatgta accattaaaa acacagatat aacattacac caaaacaatg
    11401 cagataccac tggtactcaa gaaaaaataa ctaaagacaa agatattgtg ttcacaaatg
    11461 ggggaaatgt tctgtttaag gataatttgg attttggtag cggtggtatt atctttgacg
    11521 aaggccatga atataacata aacggtcagg gatttacatt taaaggagca ggaattgata
    11581 tcggaaaaga aagcattgta aactggaatg cattgtattc cagtgatgat gttttacaca
    11641 aaataggccc cggtactctg aatgttcaaa aaaaacaggg ggcaaatata aagataggtg
    11701 aaggaaatgt tattcttaat gaagaaggaa catttaacaa tatatacctt gcaagcggaa
    11761 atggtaaggt aatactaaat aaagataatt cccttggcaa tgatcaatat gcggggatat
    11821 tttttactaa acgtggtggc acgctagatt taaatggaca caatcagact tttactagaa
    11881 ttgccgccac tgacgatgga acaacaataa ctaactcaga tacaacgaaa gaagccgttc
    11941 tggcaatcaa taacgaagac tcctacatat atcatgggaa cataaatggc aatataaaac
    12001 taacgcacaa tattaattct caggataaga aaactaatgc aaaattaatt ctggatggta
    12061 gtgtcaacac aaaaaatgat gttgaagtca gtaatgccag tcttaccatg caaggccatg
    12121 caacagagca tgcaatattc agaagcacag cgaatcattg ctccctggta tttctttgtg
    12181 gaacggactg ggtcaccgtt ttgaaagaaa cagagagttc atataataaa aagttcaatt
    12241 ctgattacaa aagtaataat cagcagacct catttgatca gcctgactgg aaaaccgggg
    12301 tgtttaaatt tgatacatta cacctgaaca atgctgactt ttcaatatca cgcaatgcca
    12361 atgttgaagg aaatatatca gcaaataaat cagctatcac aatcggcgat aaaaatgctt
    12421 acattgataa tcttgcaggg aaaaatatta ctaataatgg ttttgacttc aaacaaacta
    12481 tcagtactaa tctatccata ggagaaacta aatttacagg tggcatcact gcacataaca
    12541 gccaaatagc cataggtgat caagctgtag ttacacttaa tggtgcaacc tttctgaata
    12601 atactcctat aagtatagat aaaggagcaa aagttatagc acaaaattcc atgttcacaa
    12661 caaaaggtat tgatatctcc ggtgaactga ctatgatggg aatccctgaa cagaacagta
    12721 aaactgtaac gccgggtctc cactacgctg ctgatggatt caggctgagt ggtggaaatg
    12781 caaatttcat tgccagaaat atggcatctg tcaccggaaa tatttatgct gatgatgcag
    12841 caaccattac tctgggacag cctgaaactg aaacaccgac tatatcgtct gcttatcagg
    12901 catgggcaga gactcttttg tatggctttg ataccgccta tcgaggcgca ataacagccc
    12961 ccaaagctac agttagcatg aataatgcga tctggcatct aaatagccag tcatcaatta
    13021 atcgtctaga aacaaaagac agtatggtgc gttttactgg tgacaatggg aagtttacaa
    13081 cccttacagt ggacaacctt actatagatg acagtgcatt tgtgctgcgt gcaaatctgg
    13141 cccaagcaga tcagcttgtt gtcaataaat cgttgtctgg taaaaacaac cttctgttag
    13201 tcgacttcat tgagaaaaat ggaaacagca acggactgaa tatcgatctg gtcagcgcac
    13261 caaaaggaac tgcagtagat gtctttaaag ctacgactcg gagtattggc ttcagtgatg
    13321 taacaccggt tatcgagcaa aagaacgata cagacaaagc aacatggact ctgatcggct
    13381 ataaatctgt ggccaacgcc gatgcggcta aaaaggcaac attactgatg tcaggcggct
    13441 ataaagcctt ccttgctgag gtcaacaacc ttaacaaacg tatgggtgat ctgcgtgaca
    13501 ttaacggtga gtccggtgca tgggcccgaa tcatgagcgg aaccgggtct gccggcggtg
    13561 gattcagtga caactacacc cacgttcagg tcggtgcgga taacaaacat gaactcgatg
    13621 gccttgacct cttcaccggg gtgaccatga cctataccga cagccatgca ggcagtgatg
    13681 ccttcagtgg tgaaacgaag tctgtgggtg ccggtctcta tgcctctgcc atgtttgagt
    13741 ccggagcata tatcgacctc atcggtaagt acgttcacca tgacaacgag tataccgcaa
    13801 ctttcgccgg ccttggcacc agagactaca gctcccactc ctggtatgcc ggtgcggaag
    13861 tcggttaccg ttaccatgta actgactctg catggattga gccgcaggcg gaacttgttt
    13921 acggtgctgt atccgggaaa cagttctcct ggaaggacca gggaatgaac ctcaccatga
    13981 aggataagga ctttaatccg ctgattgggc gtaccggtgt tgatgtgggt aaatccttct
    14041 ccggtaagga ctggaaagtc acagcccgcg ccggccttgg ctaccagttt gacctgtttg
    14101 ccaacggtga aaccgtactg cgtgatgcgt ccggtgagaa acgtatcaaa ggtgaaaaag
    14161 acggtcgtat gctcatgaat gttggtctca atgccgaaat tcgcgataat cttcgcttcg
    14221 gtcttgagtt tgagaaatcg gcatttggta aatacaacgt ggataacgcg atcaacgcca
    14281 acttccgtta ctctttctga taacagcccg ggccgcgttt gcggcccttc ttctaccgga
    14341 gagaatatgt attaccctgt gacagactat atcgctcttg ctctcattat tagctttctt
    14401 tttctgacat tatttatctg cctgttatgc cttaaacatg agcgaataaa aaaagaaact
    14461 atcaggcaaa aaaatgcaca tattctggat catggctgga atgcaactga gttctcatgg
    14521 ttccgatacg gacagtataa cgaaacgggt atttacatct cgatcgagaa gaccattatc
    14581 attacaatca ccgtttctgg tgggt
//
LOCUS       DADLSB010000101        14335 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.101, whole genome shotgun sequence.
ACCESSION   DADLSB010000101 DADLSB010000000
VERSION     DADLSB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.101"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(93..218)
                     /gene="ecnA"
                     /locus_tag="JN331_002827"
     CDS             complement(93..218)
                     /gene="ecnA"
                     /locus_tag="JN331_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7260932.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(270..836)
                     /gene="efp"
                     /locus_tag="JN331_002828"
     CDS             complement(270..836)
                     /gene="efp"
                     /locus_tag="JN331_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7260933.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            878..1906
                     /gene="epmB"
                     /locus_tag="JN331_002829"
     CDS             878..1906
                     /gene="epmB"
                     /locus_tag="JN331_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7260934.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDKLLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            2301..3170
                     /locus_tag="JN331_002830"
     CDS             2301..3170
                     /locus_tag="JN331_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260935.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(3373..3726)
                     /locus_tag="JN331_002831"
     CDS             complement(3373..3726)
                     /locus_tag="JN331_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY7260936.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(3864..5519)
                     /gene="groL"
                     /locus_tag="JN331_002832"
     CDS             complement(3864..5519)
                     /gene="groL"
                     /locus_tag="JN331_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010427772.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY7260937.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMGGMM"
     gene            complement(5563..5856)
                     /locus_tag="JN331_002833"
     CDS             complement(5563..5856)
                     /locus_tag="JN331_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY7260938.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            6132..7388
                     /gene="yjeH"
                     /locus_tag="JN331_002834"
     CDS             6132..7388
                     /gene="yjeH"
                     /locus_tag="JN331_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY7260939.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(7404..7880)
                     /gene="fxsA"
                     /locus_tag="JN331_002835"
     CDS             complement(7404..7880)
                     /gene="fxsA"
                     /locus_tag="JN331_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY7260940.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            8217..9653
                     /gene="aspA"
                     /locus_tag="JN331_002836"
     CDS             8217..9653
                     /gene="aspA"
                     /locus_tag="JN331_002836"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY7260941.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            9771..11072
                     /locus_tag="JN331_002837"
     CDS             9771..11072
                     /locus_tag="JN331_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7260942.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            11188..11526
                     /gene="cutA"
                     /locus_tag="JN331_002838"
     CDS             11188..11526
                     /gene="cutA"
                     /locus_tag="JN331_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY7260943.1"
                     /translation="MLDEKSSNTASVVVLCTAPYEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            11502..13199
                     /locus_tag="JN331_002839"
     CDS             11502..13199
                     /locus_tag="JN331_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY7260944.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            13236..13811
                     /locus_tag="JN331_002840"
     CDS             13236..13811
                     /locus_tag="JN331_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7260945.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            13918..13993
                     /locus_tag="JN331_002841"
     tRNA            13918..13993
                     /locus_tag="JN331_002841"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13951..13953,aa:Phe,seq:gaa)
BASE COUNT         3491 a         3676 c         3524 g         3644 t
ORIGIN      
        1 aatgttattt atgtttgcct atagcaaatt aataatagac aacatcgcca aaaatgatgg
       61 aaatctgatg atttttcgga agagaagaaa aattagctgg cagcgcgaga gatggagttg
      121 ccgagatgtt tgatgtcttc gccgaaaccg caagcggtgt tacagcccgt gagcagcgtg
      181 ctggcaagca aaacaagaac gataaggcgt ttcatcatct ctgacctgaa taagtgatgg
      241 tgcagcctgc aggccgcacc acaaccgcat tacttcacgc gagagacgta ttcaccagag
      301 cgggtatcca ctttgatgac ttcgccgatt tgtacgaaca gcggaacttt aaccacagcg
      361 ccagtagaca gggtagccgg tttgccgcca gtacctgcgg tatcaccttt caggcccgga
      421 tcggtatcaa cgatttccag ttcaacgaag ttcggcggag taacggagat cggctgacca
      481 ttccacagag ttacgataca ctctgcctga tccagcagcc atttagcgtt gtcgccaatt
      541 gcttttgcat cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg ccagaactca
      601 ccgtcgttgt acaggtaagt caggttcata tcgacaacat cagcgccttc agcggaatca
      661 gtagatttga aggttttttc tacgcgagta ccggtcagca gacgacgcag tttaacgcga
      721 gcaaatgcct ggcctttacc cggttttacg aattcactcg cttcaaccgc gtaaggttcg
      781 ccgtctaaca tgattttaag accagcacga aaatcgttgc tatagtacgt tgccataagg
      841 ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg gcgcatattg taaccctaaa
      901 taccccatcc agagaagatt ggttaacgca acttgccgat gttgtgaccg atcctgataa
      961 acttctgcgt cttttgaata tagacgcgga cgaaaaactg ttggccggac gcagcgccaa
     1021 aaagctgttt gccctgcgtg tgccccgctc atttatcgat cgcatggaga aaggcaatcc
     1081 ggacgatcct cttttgcgtc aggtacttac ctcgcaagat gagtttgtcg tcgcgtccgg
     1141 attctccacc gacccactgg aagaacagca cagcgtagtg cctggtttgt tgcataaata
     1201 ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc gctattgctt
     1261 ccgtcgccac ttcccctatg ccgaaaatca gggcaacaag cgtaactggc agactgcact
     1321 tgagtatgtt gctgcgcatc cggaactgga cgagatgatt ttctccggcg gcgatccgct
     1381 gatggcgaaa gatcacgagc tggactggtt gctcacacaa ctggaagcca tcccgcatat
     1441 caaacgtctg cggattcaca gccgtctgcc gattgtgatc ccggcacgta tcaccgaggc
     1501 gctggttgaa cgctttgccc gttctacgct gcaaatcttg ctggtgaatc acatcaatca
     1561 tgccaatgag gtagatgaaa cgttccgtca ggcgatggct aaattgcgcc gtgtcggtgt
     1621 caccctgctt aaccagagcg ttctgttacg tggtgtgaac gataacgcac aaacgttggc
     1681 aaacctgagt aatgcgttgt tcgatgccgg cgtgatgccg tattacctgc atgtgctaga
     1741 taaagtacag ggcgcggcgc attttatggt gagtgatgac gaagcacggc agattatgcg
     1801 tgagttgctg acattggtgt cgggatatct ggtgccgaaa ctggcgcgag aaattggcgg
     1861 cgaacccagc aaaacgccgc tggatctcca gctacgccag cagtaactga ttaaacaggg
     1921 aagttaaaca ttaaaattgc ctgatgcgct acgcttatca ggcctacatc gtgcattgaa
     1981 attgaatgga tttgcaatga actgtaggcc ggataaggcg tttacgccgc atccggcaag
     2041 agcaacaaac gcaagtacag caacctgtca ttaaactgag aaatttaatc tttgcacgtt
     2101 gcataacctt taattattca tatgcattgc tgttgttact ttttatatag ctaacaccaa
     2161 taattaacct ttcttttctg tttatataga gtgagcacgg ctatttatgt tattttaaca
     2221 actattacta ccaagcccat ttatctggct tataaattaa aattaattat ttaattgaga
     2281 aatggttagg gagaacctac atggccataa gcatcaaggg tgttaatacc ggtgttattc
     2341 gcaagagtaa taatttcatt gcactggcgc tgaaaattaa agaacctcgc aataaagaat
     2401 cgttgttctt tatgtctgtc atggaactgc gcgacctgct gattgcgctg gaaagtcgtc
     2461 tgcatcaaaa gcataaactg gatgctgctg cacgccttca gtatgagcaa gctcgcgata
     2521 aagtcattaa gaaaatggca gaaaacatcc cagaaattct ggttgatgag cttaaaaatg
     2581 ccgatattaa tcgtcgggtt aacacgctgg aacttaccga taatcaaggc gaaaatctta
     2641 ccttcgtatt aacgctacat gacggtagca aatgtgagtt agtcgtcaat gagttgcaga
     2701 ttgaaatgct ggcgcgagca atcattcatg caattaataa tgcggaaatg cgtgaactgg
     2761 cattacgtat tacctcactg ttagatttcc tgccgttata cgacgttgac tgccaggaca
     2821 atggcaatct ggaatatgac acctattccc aaccggaatg gaaacataac ttgttcgacc
     2881 attatctggc ggtgctttac cgctttaaag atgaaagtgg caaagaacaa tttagcggcg
     2941 cagtggtgaa aacgcgtgaa gcaacgccgg gtaaagaaat agaggcgatc actcgccgaa
     3001 tgcttgattt cagcccacga ctgaaaaaac tcgcgggtgt gccatgtcag gtctatgttc
     3061 gcaccgtagc ggcgaataac gcccaaccgc taacccagga tcaatgctta cgcgcgctgc
     3121 atcacttacg tgtacaatcc accagcaaaa ctgcaccaca ggcgaaataa tctcatcttt
     3181 gctaatgcga ggctggagcc atccagcctg aaatgactga taacgtgatc gttggtcatg
     3241 ccggatgcgg cgtaaacgcc ttatccggcc tacaaattct caagcattca atcagttgca
     3301 atgcctgctg taggcctgat aagcgaagcg catcaggcaa taatacgttt gtcacccagc
     3361 ctctggatca catcagttcg ggcacttata aacctgaccg ataatctggc tatccgtcgg
     3421 gacaaaactg gacaacattc cctgcgacgg gctgctgatg ccataaatca cgttaccgcc
     3481 cattgcagcc gcctggttgc gcagatcgtt tgctgcgccg cgcatagaac cgccctcttc
     3541 tccgtgttgc ccggaaagcc agttgctttg cttacctgtc gcagtaccaa tcagctggca
     3601 ctctgcgcct ggctgctcgt ccacaatgcg tacactctga ccggcagcac tcaattcgtt
     3661 gctggagcta caacccgcca tcagtagcgc ggcaccgaca atccctgcta agtattttac
     3721 gtgcatgtta ttccccataa tcaatgagct ggacgccact cgcgtcgtcc gtgtctgaat
     3781 cttatactag aaagatgacc aaaagaaaaa cccccagaca tttctgcccg ggggtttgtt
     3841 tatttctgcg aagtgcaggg caattacatc atgccgccca tgccacccat gccgcccata
     3901 ccgcccatac cgccagcagc gcctaagtca gctgcatcgt ttttcggcag gtcggtaacc
     3961 atgcattcgg tggtgatcat caggccagcc acagaagctg cgtactgcag agcagaacgg
     4021 gttactttgg ttgggtccag gatacccatg tcgatcatgt tgccgtattc ttcggttgct
     4081 gcgttgtaac cgtagttgcc gtcgccgcct ttaacggtgt tagcaacaac agacggttct
     4141 tcgccgcagt tcaggacgat ctgacgcagc ggagcttcca ttgcacgcag tgcaactttg
     4201 atacccacgt tctggtcttc gttctgacca cgcaggtcag ccagtttaga cgctacgcgg
     4261 atcagcgcaa caccaccacc agcaaccacg ccttcttcta ccgcagcacg ggtcgcgtgc
     4321 agggcatctt caacgcgtgc ttttttctct ttcatttcaa cttcggtagc agcacctact
     4381 ttgataactg caacgccgcc tgccagtttc gctacgcgct cctgcagttt ttcacggtcg
     4441 tagtcagaag ttgcttcttc aatctgctga cggatctgag caacacggcc ctggattgca
     4501 gcttcttcac ccacgccatc gatgatagtg gtggtgtctt tgttgatcac aacacgttta
     4561 gcctgaccca ggtcttccag ggttgctttt tccagctcca taccgatctc ttcagagatt
     4621 acggtaccgc cagtcagggt tgcgatatcc tgcagcatag ctttacgacg atcgccgaag
     4681 cccggagctt taaccgcagc aactttcacg atgccacgca tggtgttaac aaccagagtt
     4741 gccagcgctt cgccttcaac atcttcagcg atgatcagca gcggtttgcc tgctttggca
     4801 acagcttcca gaaccggcag catttcgcgg atgttggaga ttttcttgtc agccagcagg
     4861 atgaacgggc tttccagttc tactgcgcca gtttccggct tgttgatgaa gtaaggagac
     4921 aggtagccac ggtcgaactg cataccttca accacgtcca gttcgtcctg cagaccggta
     4981 ccgtcttcaa cggtgataac gccttcttta ccgactttgt ccatcgcttc agcgatcagt
     5041 ttacctacgg tttcgtcgga gttagcggag atggtaccaa cctgagcaat cgctttagaa
     5101 tcagagcacg gtacggacag cgctttcagt tcttcaactg cagcggtaac cgctttgtcg
     5161 ataccacgtt tcaggtccat cgggttcatg cccgcagcaa cagctttcag accttcagtg
     5221 atgatagcct gagccagtac ggttgcagtg gtggtaccgt cgcctgcagc gtcgttcgct
     5281 ttggaggcaa cttctttcac catctgcgca cccatatttt cgaacttgtc ttccagttcg
     5341 atttcacgag caacggaaac accatctttg gtgatggtcg gtgcaccgaa agatttatcc
     5401 agaactacgt tacggccttt tggaccgagg gtaactttca ctgcatctgc cagtacgttt
     5461 acgccgcgca gcattttcac acgagcgtcg ttaccgaatt ttacgtcttt agctgccatt
     5521 atctttattc cttaaattcg tatgttctgt gtcgtgcgcg gattacgctt caacaattgc
     5581 cagaatgtcg ctttcggaca tgatcaacac ttcttcattg tcgatcttct cggatttcac
     5641 accgtagcca tcgttgaaaa taacgatgtc gccaactttc acgtccagcg gcttcacttc
     5701 gccattttca aggatacggc cattgccgac agccagcact tcgccgcggg tggatttagc
     5761 cgctgcagag ccggtcagaa cgatgccgcc agcagattta gtttcaactt ctttacgctt
     5821 gacgatcacg cgatcatgca atggacgaat attcattgat aactctcctt tgagaaagtc
     5881 cgtatctgtt atgggtgacg ccggagctta cgtggtttcc cggctggtga ccagagaaat
     5941 ggggatgagg cttcgcccct tcaaggggga aaaagaaaaa aaattctgat cagcacaaaa
     6001 tcgggtgaaa accctgattc acctcacatt tcatcacaat ttcttcatcg ccgtcataag
     6061 cgaatctgat tgtgctacca tcgaaaatct acgcagttgc ccaaaatttg ggcgcaatcg
     6121 gatatcaacc catgagtgga ctcaaacaag aactggggct ggcccagggc attggcctgc
     6181 tatcgacgtc attattaggc actggcgtgt ttgccgttcc tgcgttagct gcactggtag
     6241 caggcaataa cagcctgtgg gcgtggcccg ttttgattat cttagtgttc ccgattgcga
     6301 ttgtgtttgc gattctgggt cgccactatc ccagcgcagg cggcgtcgcg cacttcgtcg
     6361 gtatggcgtt tggttcgcgg cttgagcgag tcaccggctg gctgttttta tcggtcattc
     6421 ccgtgggttt gcctgccgca ctacaaattg ccgccggatt cggccaggca atgtttggct
     6481 ggcatagctg gcaactgttg ttggcagaac tcggtacgct ggcgttggtg tggtatatcg
     6541 gtactctcgg tgccagttcc agtgctaatc tacaaaccgt tattgccgga cttatcgtcg
     6601 cactgattgt cgctatctgg tgggcgggcg atatcaaacc tgcgagtatc cccttccccg
     6661 cgccaggaaa tatcgaactt accgggttat ttgctgcgtt atcagtgatg ttctggtgtt
     6721 ttgtcggtct ggaagcattt gcccatcttg cctcggaatt taaaaatcca gagcgtgatt
     6781 ttcctcgtgc tttgatgatt ggtctgctgc tggcaggatt agtctactgg ggctgtacgg
     6841 tagtcgtctt acacttcgac gcctatggtg aacaaatggc ggcggcagca tcgcttccaa
     6901 aaattgtagt gcagttgttc ggtgtaggag cgttatggat tgcctgcgta attggctatc
     6961 tggcctgctt tgccagtctt aacatttata tacagagctt cgcccgcttg gtctggtcgc
     7021 aggcgcaaca taatcctgac cactacctgg cacgcctctc ttctcgccat atcccgaata
     7081 atgccctcaa tgcggtgctt ggctgctgcg tggtgagcac gttggtaatt catgccttag
     7141 agatcaatct ggacgctctt attatttatg ccaatggcat ctttattatg atttatctgt
     7201 tatgcatgct ggcaggctgt aaattattgc aaggtcgtta tcgactactg gcggtggttg
     7261 gcgggctgtt atgcgttctg ttactggcaa tggtcggctg gaaaagtctc tatgcgctga
     7321 tcatgctggt ggggttatgg ctgtttctgc caaaacgaaa aacgccggaa aatggcataa
     7381 ccacataatc cggcgtttcg acattaatcc tggcgatcgt ctttatgatc aaggcggtcg
     7441 cggtcatcat cctttcgctg atactcacca tcaaaagtat taccgccacc ggtcccggcg
     7501 ctaaaaccgc cgcctggcat gcgagaaaag cgcaaatgtg gcatcaactt cactgtcaga
     7561 tgcttttgca ccggcggcaa taaaagcaga agaccgagga agtcggtgaa aaagcccggt
     7621 aataaaagca gcaaaccggc aataatcagc gaaacacttt taatcatctc cgccgcaggg
     7681 ttttcacccg ccgccatttt ttgctgcatc agcacgaaat tcttaaagcc ctggttacgt
     7741 accagtgaca taccgataac tgacgtgaat ataaccagca cgagggtcag caataccccc
     7801 aatacatggg caacctgaat aaagattgaa atctcaatat agacataaag gaaaatggca
     7861 ataaaaggta accagcgcaa aggtttctcc tgtaatagca gccggttaaa cccggctacc
     7921 tgaatgggtt gcgaatcgcg ttcagcttat attgtggtca ttagcaaaat ttcaagatgt
     7981 ttgcgcaact atttttggta gtaatcccaa agcggtgatc tatttcacaa attaataatt
     8041 aaggggtaaa aaccgacact taaagtgatc cagattacgg tagaaatcct caagcagcat
     8101 atgatctcgg gtattcggtc gatgcagggg ataatcgtcg gtcgaaaaac attcgaaacc
     8161 acatatattc tgtgtgttta aagcaaatca ttggcagctt gaaaaagaag gttcacatgt
     8221 caaacaacat tcgtatcgaa gaagatctgt tgggtaccag ggaagttcca gctgatgcct
     8281 actatggtgt tcacactctg agagcgattg aaaacttcta tatcagcaac aacaaaatca
     8341 gtgatattcc tgaatttgtt cgcggtatgg taatggttaa aaaagccgca gctatggcaa
     8401 acaaagagct gcaaaccatt cctaaaagtg tagcgaatgc catcattgcc gcatgtgatg
     8461 aagtcctgaa caacggaaaa tgcatggatc agttcccggt agacgtctac cagggcggcg
     8521 caggtacttc cgtaaacatg aacaccaacg aagtgctggc caatatcggt ctggaactga
     8581 tgggtcacca gaaaggtgaa tatcagtacc tgaacccgaa cgaccatgtt aacaaatgtc
     8641 agtccactaa cgacgcctac ccgaccggtt tccgtatcgc agtttactct tccctgatta
     8701 agctggtaga tgcgattaac caactgcgtg aaggctttga acgtaaagct gtcgaattcc
     8761 aggacatcct gaaaatgggt cgtacccagc tgcaggacgc agtaccgatg accctcggtc
     8821 aggaattccg cgctttcagc atcctgctga aagaagaagt gaaaaacatc caacgtaccg
     8881 ctgaactgct gctggaagtt aaccttggcg caacagcaat cggtactggt ctgaacacgc
     8941 cgaaagagta ctctccgctg gcagtgaaaa aactggctga agtcactggc ttcccatgcg
     9001 taccggctga agacctgatc gaagcgacct ctgactgcgg cgcttatgtt atggttcacg
     9061 gcgcgctgaa acgcctggct gtgaagatgt ccaaaatctg taacgacctg cgcttgctct
     9121 cttctggtcc acgtgccggc ctgaacgaga tcaacctgcc ggaactgcag gcgggctctt
     9181 ccatcatgcc agctaaagta aacccggttg ttccggaagt ggttaaccag gtatgcttca
     9241 aagtcatcgg taacgacacc actgttacca tggcagcaga agcaggtcag ctgcagttga
     9301 acgttatgga accggtcatt ggccaggcca tgttcgaatc cgttcacatt ctgaccaacg
     9361 cttgctacaa cctgctggaa aaatgcatta acggcatcac tgctaacaaa gaagtgtgcg
     9421 aaggttacgt ttacaactct atcggtatcg ttacttacct gaacccgttc atcggtcacc
     9481 acaacggtga catcgtgggt aaaatctgtg ccgaaaccgg taagagtgta cgtgaagtcg
     9541 ttctggaacg cggtctgttg actgaagcgg aacttgacga tattttctcc gtacagaatc
     9601 tgatgcaccc ggcttacaaa gcaaaacgct atactgatga aagcgaacag taatcgtaca
     9661 gggtagtaca aataaaaaag gcacgtcaga tgacgtgcct tttttcttgt gagcagtaac
     9721 ttaaaaataa caatctaata tcaacttgtt aaaaaacaag gaaggctaat atgctagttg
     9781 tagaactcat catagttttg ctggcgatct tcttgggcgc cagattgggg ggaataggta
     9841 ttggttttgc aggcggattg ggggtgctgg ttcttgccgc tattggcgtt aaacccggta
     9901 acatcccgtt cgatgtcatt tccattatca tggcggttat cgccgctatt tctgccatgc
     9961 aggttgctgg cggtctggac tatctggttc atcagacaga aaagctgctg cgccgtaacc
    10021 cgaaatacat cacgatcctc gcaccgatcg tgacctattt cctgactatc tttgctggta
    10081 ctggcaacat ctctctggcg acactgccag ttatcgctga agttgcgaag gaacaaggcg
    10141 ttaaaccttg ccgtccgctc tctactgcag tggtatccgc gcagattgcg atcaccgcat
    10201 cgccaatctc agcggcagtg gtttacatgt cttccgtgat ggaaggtcat ggcatcagct
    10261 acctccatct gctctccgtg gtcatcccgt ccaccctgct ggcggttctg gtgatgtcct
    10321 tcctggtcac tatgctgttc aactccaaac tctctgacga tccgatttat cgcaagcgtc
    10381 tggaagaggg cttggttgaa ctgcgcggtg aaaagcagat tgaaatcaaa tccggtgcaa
    10441 aaacgtccgt ctggctgttc ctgctgggcg tagttggcgt ggttatctat gcaatcatca
    10501 acagcccaag catgggtctg gttgaaaaac cgctgatgaa caccaccaac gcaatcctga
    10561 tcatcatgct cagcgttgca actctgacca ccgttatctg taaagtcgat accgacaaca
    10621 tcctcaactc cagcaccttc aaagcaggta tgagcgcctg tatttgtatc ctgggtgttg
    10681 cgtggctggg cgatactttc gtttccaaca acatcgactg gatcaaagat accgctggtg
    10741 aagtgattca gggtcatccg tggctgctgg ccgtcatctt cttctttgct tctgctctgc
    10801 tgtactctca ggctgcaacc gcaaaagcac tgatgccgat ggctctggca ctgaacgttt
    10861 caccgctgac cgctgttgct tctttcgctg cggtgtctgg tctgttcatt ctgccgacct
    10921 acccgacgct ggttgctgcg gtacagatgg atgacacggg tactacccgt atcggtaaat
    10981 tcgtcttcaa ccatccgttc ttcatcccgg gtactctggg tgttgccctg gccgtttgct
    11041 tcggcttcgt gctgggtagc ttcatgctgt aatgatcaat cgcggggcgt tcacgccccg
    11101 ctttctttcc cgccgactaa catccttccc ccgtccgttg tatagtgacc tctctcttgc
    11161 ggttccatct gttcttgcga ggtgtttatg cttgatgaaa aaagttcgaa taccgcgtct
    11221 gtcgtggtgc tatgtaccgc accgtatgaa gcgacagccc aggatttagc cgccaaagtg
    11281 ctggcggaaa aactggcggc ctgcgcgacc ttgatccccg gcgctacctc tctctattac
    11341 tgggaaggta agctggagca agaatacgaa gtgcagatga ttttaaaaac taccgtatct
    11401 caccagcagg cactgctgga atgcctgaag tctcatcatc catatcaaac cccggaactt
    11461 ctggttttac ctgttacaca cggagacaca gattacctct catggctcaa cgcatcttta
    11521 cgctgattct gctactttgc agcacttccg tttttgccgg attattcgac gcgccgggac
    11581 gttcacaatt tgtccccgcg gatcaagcct ttgcttttga ttttcagcaa aaccaacatg
    11641 accttaatct gacctggcag atcaaagacg gttactacct ctaccgtaaa cagatccgca
    11701 ttacgccgga acacgcgaaa attgccgacg tgcagctgcc gcaaggcgtc tggcatgaag
    11761 atgagtttta cggcaaaagc gagatttacc gcgatcggct gacgcttccc gtaaccatca
    11821 accaggcgag tgcgggagcg acgttaactg tcacctacca gggctgtgct gatgccggtt
    11881 tctgttatcc gccagaaacc aaaaccgttc cgttaagcga agtggtcgcc aacaacgcag
    11941 cgtcacagcc tgtgtctgtt tcgcagcaag agcagcacac cgcgcaattg cccttttccg
    12001 cgctctgggc gttgttgatc ggtattggta tcgcctttac gccatgcgtg ctgccaatgt
    12061 acccactgat ttctggcatc gtgctgggtg gtaaacagcg gctctccact gccagagcat
    12121 tgttgctgac ctttatttat gtgcagggga tggcactgac ttacacggcg ctgggtctgg
    12181 tagttgccgc cgcagggtta cagttccagg cggcgctaca gcacccatac gtgctcattg
    12241 gcctcgccat cgtctttacc ttgctggcga tgtcaatgtt tggcttgttt actctacaac
    12301 tcccctcttc gctgcaaaca cgtctcacgt tgatgagcaa tcgccaacag ggcggctcac
    12361 ctggcggtgt gtttgttatg ggggcgattg ccggactgat ctgttcacca tgcaccaccg
    12421 caccgcttag cgcgattctg ctgtatatcg cccaaagcgg gaacatgtgg ctgggcggcg
    12481 gcacgcttta tctctatgcg ttgggcatgg gcctgccgct gatgctaatt accgtctttg
    12541 gtaaccgctt gctgccgaaa agcggcccgt ggatggaaca agtcaaaacc gcgtttggtt
    12601 ttgtgatcct cgcactgccg gtcttcctgc tggagcgagt gattggtgat gtatggggat
    12661 tacgcttgtg gtcggcgctg ggtgtcgcat tctttggctg ggcctttatc accagcctac
    12721 aggctaaacg cggctggatg cgcgtggtgc aaataatcct gctggcagcg gcattggtta
    12781 gcgtgcgccc acttcaggat tgggcatttg gtgcgacgca taccgcgcaa actcagacgc
    12841 atctcaactt tacacaaatc aaaacggtag atgagttaaa tcaggcgctc gttgaagcca
    12901 aaggcaaacc ggtgatgtta gatctttatg ccgactggtg cgtcgcctgt aaagagtttg
    12961 agaaatacac cttcagcgac ccgcaggtgc aaaaagcgtt agcagacacg gtcttacttc
    13021 aggccaacgt cacggccaac gacgcacaag atgtggcgct gttaaagcat cttaatgtcc
    13081 ttggcctacc gacaattctc ttttttgacg gacaaggcca ggagcatcca caagcacgcg
    13141 tcacgggctt tatggatgct gaaaccttca gcgcacattt gcgcgatcgc caaccgtgaa
    13201 cgacacttgc agtgggaaag acggaggaaa atagcgtgca acgtgaagat gtactgggag
    13261 aagccctgaa attattagaa ttacaaggga ttgccaacac cacgctggag atggttgctg
    13321 aacgtgtgga ttatccactg gacgagctac gccgcttctg gccagacaaa gaggcaatcc
    13381 tctacgatgc gctgcgttat cttagccaac agatagatgt ctggcgtcgt cagctgatgc
    13441 tggacgaaac gcaaaccgcc gaacaaaagc tgctggcacg ttatcaggcg ttatcggagt
    13501 gcgttaaaaa caaccgctat ccgggctgtc tatttatcgc tgcctgtacg ttttatcccg
    13561 atcctggcca ccctattcat caactggccg atcagcaaaa aagcgcggcc tacgatttca
    13621 cccacgaact gttaaccacg ctggaagttg acgatccggc gatggtagca aagcagatgg
    13681 aactggtgct ggaaggctgt ttaagccgaa tgctggtgaa tcgtagccag gcggatgtcg
    13741 acactgcaca tcggctggcg gaagatatcc tgcgcttcgc ccgctgccgc cagggtggtg
    13801 cattgacctg acagaaacac agaaaagaag cgatttgccg caatcttaag cagttgaatc
    13861 gcttttactg aaattaggtt gacgagatgt gcagattacg gtttaatgcg ccccgttgcc
    13921 cggatagctc agtcggtaga gcaggggatt gaaaatcccc gtgtccttgg ttcgattccg
    13981 agtccgggca ccaaattcat atcaacggac ctccacggag gtccgttttt cgtttcagga
    14041 cgccacgatt taagcgttct gcctccaaat caattctacc gaactcaacc agattctccc
    14101 cacatcacca gcaatttgcg agcatatccc aatttgggaa aatttgttcc tgagctatag
    14161 cactaactga cgtgaaatgt cgtgcggccc cgtgatgctg ttgaacgtca aatgacgtca
    14221 tcaggagcgt aacgcatcca taaagcacaa caccggtctg aacgccgact gatgagattt
    14281 tctgaatgag aacaaagaga aatgtgtcag tccgcttgct catgcaaaga ctaac
//
LOCUS       DADLSB010000102        13753 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.102, whole genome shotgun sequence.
ACCESSION   DADLSB010000102 DADLSB010000000
VERSION     DADLSB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.102"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(17..763)
                     /gene="dsbG"
                     /locus_tag="JN331_002842"
     CDS             complement(17..763)
                     /gene="dsbG"
                     /locus_tag="JN331_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY7260946.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            1135..1698
                     /gene="ahpC"
                     /locus_tag="JN331_002843"
     CDS             1135..1698
                     /gene="ahpC"
                     /locus_tag="JN331_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY7260947.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1943..3508
                     /gene="ahpF"
                     /locus_tag="JN331_002844"
     CDS             1943..3508
                     /gene="ahpF"
                     /locus_tag="JN331_002844"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY7260948.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRASGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3629..4057)
                     /gene="uspG"
                     /locus_tag="JN331_002845"
     CDS             complement(3629..4057)
                     /gene="uspG"
                     /locus_tag="JN331_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY7260949.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            4278..5516
                     /locus_tag="JN331_002846"
     CDS             4278..5516
                     /locus_tag="JN331_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HAY7260950.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5747..6157)
                     /gene="rnk"
                     /locus_tag="JN331_002847"
     CDS             complement(5747..6157)
                     /gene="rnk"
                     /locus_tag="JN331_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY7260951.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6386..7192)
                     /gene="rna"
                     /locus_tag="JN331_002848"
     CDS             complement(6386..7192)
                     /gene="rna"
                     /locus_tag="JN331_002848"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY7260952.1"
                     /translation="MKAFWRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            complement(7306..8769)
                     /gene="citT"
                     /locus_tag="JN331_002849"
     CDS             complement(7306..8769)
                     /gene="citT"
                     /locus_tag="JN331_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY7260953.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIIGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8820..9698)
                     /gene="citG"
                     /locus_tag="JN331_002850"
     CDS             complement(8820..9698)
                     /gene="citG"
                     /locus_tag="JN331_002850"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY7260954.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTIVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9673..10224)
                     /gene="citX"
                     /locus_tag="JN331_002851"
     CDS             complement(9673..10224)
                     /gene="citX"
                     /locus_tag="JN331_002851"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY7260955.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRIEALLNDVDACNVN"
     gene            complement(10228..11759)
                     /gene="citF"
                     /locus_tag="JN331_002852"
                     /pseudo
     CDS             complement(10228..11759)
                     /gene="citF"
                     /locus_tag="JN331_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11770..12678)
                     /gene="citE"
                     /locus_tag="JN331_002853"
     CDS             complement(11770..12678)
                     /gene="citE"
                     /locus_tag="JN331_002853"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY7260956.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12675..12971)
                     /gene="citD"
                     /locus_tag="JN331_002854"
     CDS             complement(12675..12971)
                     /gene="citD"
                     /locus_tag="JN331_002854"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY7260957.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12986..>13753)
                     /gene="citC"
                     /locus_tag="JN331_002855"
     CDS             complement(12986..>13753)
                     /gene="citC"
                     /locus_tag="JN331_002855"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY7260958.1"
                     /translation="KTEYEALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFR
                     HPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKG
                     TADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHR
                     FVGTEPFCRVTAQYNQDMRYWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAK
                     NDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3368 a         3494 c         3660 g         3231 t
ORIGIN      
        1 atccatgaat taccttttat ttatttccca taatgatatt aagcgttttc tgatcgggca
       61 accccacggc ctgttgcagc gtattttcct tactcatgta atagatagcc ggcgtgacat
      121 ttgcccccag atcgtccatc agtttctcat tgtcacttaa cactttcatt tgctctgtac
      181 ttacgtttgc aggcacgttt agcttaagct tgcaaccaga ggcttcatat tgttgccagg
      241 tttttgcggg atctttggag gcaagaattg ccgctgctgt cgccgggctt tctggcttga
      301 taaccccaac caacaatgtt cttaattgca ctttgccaga atctacccac gggcgcgcct
      361 gctgccagaa ctgtttacaa tatgggcaga acggatcggc gaagacgtag acaatgaccg
      421 gcgcatcttt tttaccgtcg aggagccagt gggattgttc catccgttgc cacatttcgc
      481 gtccggctgg tgcgtaaatt tctttttcga taagtgtgtt actcaggttt tcacctttct
      541 cgttgtacat gtaaccagag atagcgtgct taccatcggg agtcaggtag atggtgacgc
      601 ccatatcctg atactttccg agataacctt tcattcctcc gggggcatcg aacgttttga
      661 tgattgtaat gccctgtttt tcaatcgctt ttactgggga aggaagttcc tctgcgaagg
      721 cgattgcagg aagcagagcc agtaaaagta tcttttttaa cattaatttg tccttttcag
      781 tcagtgcaaa agtcgagtaa aaggcataac ctatcactgt cataggtaag agcttagatc
      841 aggtgattgc cctttgttta tgagggtgtt gtaatccatg tcgttgttgc atttgtaagg
      901 gcaacacctc agcctgcagg caggcgctga agataccaaa gggtagttca gattacacgg
      961 tcacctggaa agggggccat tttacttttt atcgccgctg gcggtgcaaa gttcacaaag
     1021 ttgtcttacg aaggttgtaa ggtaaaactt atcgatttga taatggaaac gcattagccg
     1081 aatcggcaaa aattggttac cttacatctc atcgaaaaca cggaggaagt atagatgtcc
     1141 ttgattaaca ccaaaattaa accttttaaa aaccaggcat tcaaaaacgg cgaattcatc
     1201 gaaatcaccg aaaaagatac cgaaggccgc tggagcgtct tcttcttcta cccggctgac
     1261 tttactttcg tatgcccgac cgaactgggt gacgttgctg accactacga agaactgcag
     1321 aaactgggcg tagacgtata cgcagtatct accgatactc acttcaccca caaagcatgg
     1381 cacagcagct ctgaaaccat cgctaaaatc aaatatgcga tgatcggcga cccgactggc
     1441 gccctgaccc gtaacttcga caacatgcgt gaagatgaag gtctggctga ccgtgcgacc
     1501 ttcgttgttg acccgcaggg tatcatccag gcaatcgaag ttaccgctga aggcattggc
     1561 cgtgacgcgt ctgatctgct gcgtaaaatc aaagcagctc agtacgtagc ttctcaccca
     1621 ggtgaagttt gcccggctaa atggaaagaa ggtgaagcaa ctctggctcc gtctctggac
     1681 ctggttggta aaatctaaat ttccttcgtc tttcacgcca tagcggcgtt ggcgtcgccc
     1741 gctcaccccg gtcacttact tgtgtaagct cccggggatt cacaggctag tcgccttgct
     1801 ctgacgcgaa atacttcgga aattcaccta attcttcggg tgctgcggca cccgattttc
     1861 ttccccgcac catgatgcaa gctgcatcca ggtagccgca gaggccgctt gcatgatgat
     1921 gtttaaagcc caggagataa acatgctcga cacaaatatg aaaactcaac tcaaggctta
     1981 ccttgagaaa ttgaccaagc ctgttgagtt aattgccacg ctggatgaca gcgctaaatc
     2041 ggcagaaatc aaggaactgt tggctgaaat cgcagaactg tcagacaaag tcacctttaa
     2101 agaagataac agcttgccag tgcgtaagcc gtctttcctg atcaccaacc caggttccaa
     2161 ccagggacca cgttttgcag gctccccgct gggccacgag ttcacctcgc tggtactggc
     2221 gttgctgtgg accggtggtc atccgtcgaa agaagcgcag tctctgctgg agcagattcg
     2281 ccatattgac ggtgattttg aattcgaaac ctattactcg ctctcttgcc ataactgccc
     2341 ggacgtggtg caggcgctga acctgatgag cgtattgaac ccgcgcatca agcacactgc
     2401 aattgacggc ggcaccttcc agaacgaaat caccgatcgc aacgtgatgg gcgttccggc
     2461 agtgttcgta aacgggaaag agtttggtca gggccgcatg acgttgactg aaatcgttgc
     2521 caaaattgat actggcgcgg aaaaacgtgc ggcagaagag ctgaacaagc gtgatgctta
     2581 tgacgtatta atcgttggtt ccggcccggc gggtgcagcg gcagcaattt actccgcacg
     2641 taaaggcatc cgtaccggtc tgatgggcga acgttttggt ggtcagatcc tcgataccgt
     2701 tgatatcgaa aactacattt ctgtaccgaa gactgaaggg cagaagttgg caggcgcact
     2761 gaaagttcac gttgatgaat acgacgttga tgtgatcgac agccagagcg ccagcaaact
     2821 gatcccggca gcggttgaag gtggtctaca ccagattgaa acagcttctg gcgcggtact
     2881 gaaagcacgc agcattatcg tggcgacggg tgcaaaatgg cgcaacatga acgttccggg
     2941 cgaagatcag tatcgcacta aaggcgtgac ctactgcccg cactgcgacg gcccgctgtt
     3001 taaaggcaaa cgcgtagcgg ttatcggcgg cggtaactcc ggcgtggaag cggcaatcga
     3061 cctggcgggt atcgttgagc acgtaacgct gctggaattt gcgccagaaa tgaaagccga
     3121 ccaggttctg caggacaaac tgcgcagcct gaaaaacgtc gacattattc tgaatgcgca
     3181 aaccacggaa gtgaaaggcg acggcagcaa agtcgttggt ctggaatatc gagatcgtgc
     3241 cagcggcgat attcacaaca tcgaactggc cggtattttt gtccagattg gtctgctgcc
     3301 gaacaccaac tggctggaag gcgcagtcga acgtaaccgc atgggcgaga ttatcattga
     3361 tgcgaaatgt gaaaccaacg tcaaaggcgt gttcgcagcg ggtgactgta cgacggttcc
     3421 gtacaagcag atcatcatcg ccactggcga aggtgccaaa gcctctctga gtgcttttga
     3481 ctacctgatt cgcaccaaaa ctgcataaga agaagtaaga ttcacctgca attgcttagc
     3541 cgccggggtc aaacctggcg gcttttttat ggcattaaaa agccctgccg ggatgttcca
     3601 ggcagggcgg cataaatact tttatctgtt agcgaacgac cagtacaggc agattggcgt
     3661 ggcggatcac gctcgaggcg ttagaaccta acagatgggt cgaaatcgat gggttgcgag
     3721 aaccgataac tacaacatca gcccccagtt cttctgccaa ctcattgact tcatcccgca
     3781 cgctaccaaa acggacatgt tgtttaatgc gggaaggatc gatggtgaag tggctgacca
     3841 tcgtttgcag acgttcttct gcttcatgtt gcagatgctc ttcaaaacga cgcacatcag
     3901 cggcaaaacg gtgcaggctc aggctggctg acccgggtag tacgtgaagt agatgaataa
     3961 ctccgtcatc ctgggcgagg aattcagcgt ggcgaacagc tttgtcgctc aattccattt
     4021 caaatacatc aactggcata atgattgtct tatacataac cctttctccc tgttaatcat
     4081 gaacaaatca ttcgccatga ttataatatt tatccctgat atttgtctgg ttctttttcc
     4141 ttacgaacgg tttctgtgat taatatattc tcactgaaca ccaggaattc tcccaaaacc
     4201 tgcggtaccg cccgttttcc cgctgtgata gctaccctta aagactgact cttttttgaa
     4261 ctgtctctgg aggttgcatg aaagcattga cttatcacgg cccacatcac gttcaggtag
     4321 aaaatgttcc cgatccgggc attgaacagg cagatgatat tattctgcgt attacggtaa
     4381 cggcgatctg tggctctgac ctccatcttt atcgaggcaa aataccccag gttaaacatg
     4441 gcgatatttt tggtcatgaa tttatggggg aagtcgttga aaccgggaag gacgtaaaaa
     4501 atttgcaaaa aggcgaccgg gtggtaattc cgttcgtcat tgcttgtggc gactgttttt
     4561 tctgtcgatt acagcaatat gccgcctgcg aaaataccaa tgcgggtaaa ggcgctgcgc
     4621 tcaataaaaa acagatacct gctcccgcgg cattgtttgg ttatagtcac ctgtatggtg
     4681 gcgttcctgg cgggcaggcg gaatatgtcc gcgtccctaa agggaatgtg gggccgttta
     4741 aagtaccgcc tttgctttca gatgataaag cccttttcct ttctgatatt ctgccaacgg
     4801 catggcaggc agcaaaaaat gtgcagatcc aacaaggttc aagcgttgca gtctatgggg
     4861 ctggtcctgt gggattgttg acaatcgcct gtgcacggtt gctcggtgcg gaacagattt
     4921 ttgttgttga tcatcatccc taccgcttgc gtttcgccgc tgaccgctac ggcgcgatcc
     4981 cgattaattt cgatgaagat agtgatccgg cacagtcaat tattgaacaa acggcaggtc
     5041 accggggcgt ggatgcagta atagacgccg tcggttttga agcgaaaggc agcaccacgg
     5101 aaacggtgct gactaacctg aaactggagg gcagcagcgg taaagcgttg cgccagtgta
     5161 ttgcggcggt caggcgtggc ggcattgtta gcgtaccggg cgtctacgct ggatttattc
     5221 acggtttcct gtttggcgac gcctttgata aagggttgac gtttaaaatg ggacagaccc
     5281 acgttcacgc atggctggga gaattattac cgttaattga gaaaggatta ctgaaaccag
     5341 aagaaattgt tacccactat atgccgtttg aagaggccgc ccggggatat gagattttcg
     5401 aaaaacgtga agaggagtgc cgtaaggtga ttctggtacc cggtgcacaa agcgcagagg
     5461 cggcgcagaa ggcggtttca ggtctggtga atgcgatgcc ggggggaaca atatgatcgt
     5521 caggagtggt tttcgaggta aaggacagcc atgacaataa tcgccgccat aatcagaaat
     5581 cctatcagga tgtaaaatgc ttctgccatg gttattccca caaacgaaac ggaataattt
     5641 tgcagcaaaa gtgaacagtg agaaccagga aaaattgctg attttgcgta aagaggatgc
     5701 gagtgcatcc tctgggcaaa gcgagttatc gcttgtgcag atgggattaa agcaggtagt
     5761 cgccagcagc ttctggctgg tattcgagtt ccagcacttc aaggtgggtt gcaacgccgc
     5821 ccggaagttc ccagtgaatg gaatcgccaa cgcgcagccc cagcagtgcg gcacctaccg
     5881 gagccataac ggaaagctga gtattgctat cggtcatttt tgccggatac accagcgtgc
     5941 gcacacgcac ttcgccatcg ctaagattgc ggaatttaac ccggctgttc attgtcacca
     6001 cgtcgtgtgg catctcttct ggcgaacaca tttgggcgcg atccaactct gcgtttaacg
     6061 cgtcggcgat tggcaaacca gcataggcgg gttgctccag cagaatatcg atgcgttcgg
     6121 catccaggtc gttaatgatg atagttggtc tggacatttt tactccatgt cgtcggtact
     6181 gcgagtgtcg cagataaaca tacccaaaag aaaaccctca ccgtcaggcg gcgagggttt
     6241 aactcacatg atgatactga ctgttgctca ctctttgaag tgatttgcgt cacattcagg
     6301 gaattcctca atgcagcgca ttatgtataa ttcttaatcg gcatggttat ggaagacgaa
     6361 ttagcgtgtt ttgtaaatca gaggattaat aacccgcttt atcaatcaca aaggttttgc
     6421 cacagttacc tgggtgaggt tgtggcaaga atgagtttgc agaaagcgga gcgttgatgg
     6481 cgtcagcttt gatcgagatc tgaatttcag tcaaatacgc agggttaccc tggcacgtta
     6541 gcttaactgc tttcacgttc tcttttcccc agcttttggc aaaggcggcg tcaaagtcac
     6601 ggcggctcac tgttttaccg taattatccg caaggaattt tccggcttcg ctctctttaa
     6661 tttcttgatt caggcgtacc atcgtaccga agtatgcgtc cggatcaaaa ccaaagcagg
     6721 caccgtgttt ggcatattcg tagcgttcca ggcaggaacg tccgccagct cctggcatga
     6781 cttcacttag tttagcggcc gtttccagtg ataacccggt ttccggcgat gaacacattc
     6841 ggctggcgcg tgcttctggt agattcggga ttgggcgagt agcgcaaccg aagcgcatcc
     6901 agcggcgttc atcaacacca cgggcagcaa ccgatttagg caatcctggc cacagaccat
     6961 gtacggtcag aaaatcagct ttgttggtcg tttcggtttg caggcgacat tcatctcgtt
     7021 cgttacgatt tcgatcgtgt tgactctggc aaaatccggt ttgccaggag agggccagga
     7081 catagcgatc aaaatcgcca tactgttttg cctgcaacgc tacggcgttg gcagaagata
     7141 agggaagcag agaaaccgcg agcaacgcgg cgttacgcca gaatgctttc ataatgggtg
     7201 tggaactcat acatacactg aatactatct attaaatcat aaaaaagccc gccaggtggg
     7261 cttacctggc gggcgtgatg atttattcag cgtttggcga acgtattagt tccacatggc
     7321 gagaatcggc cagccaacca acagcaacat agagatgtaa atcaccccga agattgcgcc
     7381 aagacgccag taatcttttg atttcacata gccacagccg taaataatca ccccaggacc
     7441 ggttgcatac ggcgtcagac agcctatgat accgatagac agcaccagca ggatacacag
     7501 ttgttccatt ggtacgcccg gaataccttt accgacggcc agaataaccg gcagcatggt
     7561 tgcggtgtgc gcagacaggc tggcaaacag gtagtgtgca aagtagaaca ccagaaccag
     7621 tacaatcacc gttgcgtttg gtgagaatcc ttccaggtgc gtactcatgg taccggcgaa
     7681 ccagtcaata aaaccagaac gagtcaggcc gttagccatc acaaccagag ttgccaggtt
     7741 gaccagcgtg ttccatgcgc tgttatagcg ggtaatgtct ttccacggca caacgtgcag
     7801 agccagcatt agcgaaactg ccagcagacc aaccgcagta gcattaatga cttcactgcc
     7861 aaatacccac aaacctaagc tgagcaatac aaggccaatc agcgtccact ctctgcgtgt
     7921 cagcgcaccc atggttttta gttcatcacc cgcccaggtt gccacttctt cactgtgtgt
     7981 gatttccggt ttgtacagca cgtaggaaag ccacggcgca atgataagca agataacccc
     8041 aaccggcagg aagcagagga accactgcaa ccagctaatc tggataccgg caattttgct
     8101 gacgaactcc agacccagca cgtttggtgc cgcaccggtg acaaacatgg acgaactcag
     8161 actggtacta atgaccatca tccacatcaa atagccgcca atacgacgcg cggacggatc
     8221 gttcgggaat gatttaaaca acggcggcag gtttttaatg accggaaaaa ccgtaccccc
     8281 ggtacgcgcg gtgttggacg gtgtaaacgg tgccagcaga atgtcgataa tgacaatcgc
     8341 ataacccaac gtcagcgtgc gtttgcccat gaatttcact aggaaaaggg caatgcgacg
     8401 acctaacccg gaaacttcat accctaatgc aaaaataaat gcgccaaata ccagccatac
     8461 cgtggtgctg gaaaaaccag ccaggcccca tttcagcgcc tgtttttgcg cattaaacgc
     8521 tgggtcagct aattctttgg catcaaagag caggtaatta ctgccaataa cgcaaatagt
     8581 aaccgcaata aaactgatcg ctgttgccgg aattggctcg aggatcatgc cgacaatcat
     8641 tgccacaaac acagcgaagt aatgccatgc ctgcggcggc ataccgtcgg ggacagggat
     8701 aagaaacatg acacccatca ccaccagtgg ggccaatagt ttccatatat tatcttttgc
     8761 taaagacata cgggttctcc gaaaattaat atttccaaat ttatcaagtg cttaaataat
     8821 taaatctgtg ctaaaaacca ggtaaggatc agcaggtcgg cactgccgcc tggactgaga
     8881 tttcgttcga tacactccct gtcgaactgc cggagataat cgagatcggc gggggttcga
     8941 atgccccctt tttgcaataa tgtttgcgcc tcgcgctgta gccagcgcag gcccccctcg
     9001 ccaccgcgcg atgcaacgtt ggtatcgccg ttggtagcca tcagtaggag caaggtatcg
     9061 agcaatgcca gttcaggatc taacccctga tccagcagag tgaggtaatg cggcaaggcg
     9121 tggttgatca ccagtggata acccgcttcg gcttcaccgc gtgcgccggt aaggccaagc
     9181 tgctggtaca accgttgacc tgccgtcagt cgtgaattat tggtacgcag ttcgcgatcg
     9241 gtcaggccac ggcagaaact tgccgccgta gaacaaacga ttgttggcgt aaccggctgg
     9301 ttgagctgaa gcaaacggcc aattgccgca catagcagcc ctaaagaaaa aatgctgcct
     9361 ttatgcgtgt ttacgcccgc agtggcgcgg aacatatcac cttcgcaagc cataccaatt
     9421 gggcgtaatc cgtggagtac cgcttccggt gccatttctg cactacaggc accaaattca
     9481 atgaaacggg gtaaccagcc ctgaatcgcc agcgcgctgc ggtggaaatc ttccagcgcc
     9541 atatctttgt gcgcaccgca gttaatgcga tccacgaggc ctggtttcgg tgacagattg
     9601 acttcagtca gcatggcgcg ccagcccagc agggcgtact catcgattaa tgacgtcgca
     9661 agctttgtgg ttttagttga cgttgcaggc atcgacatcg ttcagcagtg cctctatgcg
     9721 gttgagtaaa tcggtcagtt gatgggtttt tccacgcgcg cagacggctg cgctttgctc
     9781 gcacaacagg cagcggcgag gcggcagtga atagtcgcgg cgggagagaa tttcgccttc
     9841 gggcgtcagg acatcgatat cccataaccg cccgagagga tgactatgtt caagctcaat
     9901 ggtggcgagc ttgaggtcgc gagccggggc ggcaatgctc aacatgccct ccggcccgct
     9961 ggcggaaacc agtgcagcct gctcctgaat ttgccagccc tgctttgtgg ctaaggcacg
    10021 caaggctgtc acgccatgat taaaaattcg gcgtgtgacc tcgctgtctt taatcggccc
    10081 aggcgcaacc acggtaaagg agaccagtgg aacaggatgg tgcttgagcc aggcgtgttg
    10141 ccgtgcttgc ctttcatccc ggctgacgag cagctcggga attgacaccg catggtggct
    10201 ggcgagttca ggaagcaggt gcatggctta ttccttcacc tgatgcacaa catcgatcac
    10261 cgagccatcg cggtaacgca caacggcaac gacgcggtcg gtgaattcaa tcggctgtgg
    10321 ttcaccggtc agcagacgcg cacgttcgcg cagccactca atggaaacca ctttaatgcc
    10381 cgcttcctgc agacgttctg ctagttccgg acgtgccggg ttaactgcga taccgtggtc
    10441 tgtgaccaga atatcgacac tggagcccgg ggtgatgcag gtcagcacgt tatccaccag
    10501 agtcggaata cgaccgcgta ccagtggcgc gacgatgatg gaaagcgcag aggcaatcgc
    10561 ggtatcgcag tgaccaccgg aagcgccacg cagtacgccg tcagaaccgg tcagcacgtt
    10621 aacgttgaac tgcctgtcaa tttccagcgc gctcagtacc accacgtcga gacgatcaac
    10681 cgatgcgcct ttcgacccca gttagcgtac tggttggcgc tgatttcgat gtgattgggg
    10741 ttacgggcca gcgattgcgc agcatggctg tcaaagctct gcacatccag cagtttgcgg
    10801 atcagacctt tttcgtgcag atcaaccatc gtcgcggtaa taccgccgag ggcgaagtcg
    10861 gcgcgaatat cgcggctacg cattttgtct tccaggaaac gggttaccgc cagcgaagcg
    10921 ccgccggtgc cggtttgcat ggagaaacct tctttgaagt agccagagtt gacaatcaca
    10981 tccgcagcgc tacgggcaat aagcagttcg cgcgggttag tggtcatacg ggtagtacca
    11041 gcgccgattt ttgcagcatc gccaacgcga tcaactttga cgatcaaatc aacctgatct
    11101 tgctcaatgc ttgccggatt atgcggataa ggcagcagtt cttcggtaag catcacgacc
    11161 tgttttgcgt tgtcggcatc aaccattgca tagccgaggg agccgcagca ggctttaccg
    11221 gtgtagccgt tggcattacc gaattcatca caggacggga cgccgaggaa agccacgtcg
    11281 atattcagtt cgccgctctg taccagatgc acacgaccgc cgtgagagtg gatctgcacc
    11341 ggttccgcca gcagaccacg ggagatctct tccgccagtg gaccgcgtag gccagaggta
    11401 taaatgcggg taaccacgcc ctggcgaatg tgttctacca gcggcacatg gcaatcactc
    11461 agggagctgg acgccagggt caggttttta aagcccatct tcgcgatgac gtccatcacc
    11521 atattgacgg tcaggtcacc gccacggaaa gcgtgatgga aggaaaccgt catgccgtcc
    11581 tgtaaacctg agcgacgaat cgcttcttcc aggttggcgc acagtttgcg atcgcgcgct
    11641 ttttcagcct ggtaggtttg ctttggtgag ttctggaaag cggcaagatc gcattcagtg
    11701 cgacgattcc aggccgctac ccgttcttgt cgttgagatt gttcaatttt ctgcgtcatt
    11761 ttgattgcct tattcttcgc ggatgccgga aagttctgca cgggagagca ccagacgggc
    11821 gcgatcgata accggaccgt ccaccatctt gccgttcagg gaaaccacgc cgaggccttc
    11881 gcgagcggcg gcttcagcgg cttctacgac gcggcgggcg tgatccactt ctttctgggt
    11941 cggtgcgtag aggttgtgca gcagatcaat ctgacgcggg ttgatcagcg atttgccgtc
    12001 aaagcccagc tgtttgatgt gggcggcttc ttgcaggaat ccggcttcgt tgttagcgtc
    12061 ggaatagacg gtatcgaacg cctgaatacc cgcagagcgc gcggcctgca aaatggaaca
    12121 gcgtgcgaac agcagttcag ttccttccgg ggagcgttct gtacgcaggt tgcgcacata
    12181 gtcttctgca ccgagggcga taccgatcaa acgctcggaa gcgtgagcga tttccactgc
    12241 gcgggtaatg cccagcggag attcaatcgc cgccagcagg ccggtgctgc cgggttcacg
    12301 accacaggct ttttctatac gcaggatctc tttttcaata tccagaacat cctgagcggt
    12361 atcggttttc ggcagacgca caacgtccgc accaccgcga acgacggctt ccaggtcgtt
    12421 aacaccccat tcggaatcca gcgcgttgac acgcacaatg gtttcaatat cgcgatacag
    12481 cggatgttgc agcgcgtgat aaaccatgcg gcgggcggtg tctttttcac gtaatgccac
    12541 ggagtcttcg aggtcaaaca tcagggcatc agccgggtag atgaaggagt tgctgaccat
    12601 cgcggcattg gcaccaggca caaacaacat gctgcggcgg gtgcgagttt tacgttgttg
    12661 cagcgaagcg gaaatcattg gcaatcctcc catggcagag ccgggatacc gctggcgcgt
    12721 gccagcaggg cttccagtcg tgcacgtaaa atgcagtcca gtgcgccttt gtcatcgaca
    12781 ttcagctgta cgccgcgcac gttgtagcgg gcgagaacgt ccagaatggt ggtgcgaatt
    12841 gcatcgccaa actgtttctc aacgctgcta ttgatttgca ggtcgatatc ctgcgtatcg
    12901 agtggggcga tgcgtatcat cacatcccca gactcaaggg tgcctgcaac ggcgggctgg
    12961 tttattttca tttttcacct gtttctcatg cgggggtctt ttgacgagct gccgcgtcct
    13021 ggcgggagtg ctcaagcagg ttctgcaaat aatgcagcgt gactgcaggg accagcggcg
    13081 cgatagccgt gagatcgttt ttcgccagca gttgacgtac ccgggaagcg gatatcggca
    13141 tctcctggta acgcagccgc tcaatttcaa ccagttcgat gggcgctgcg gagatagtcg
    13201 gcgtttccag ccagtagcgc atatcctggt tgtactgggc ggtaacgcga caaaagggtt
    13261 cagtaccgac aaagcggtga gttacaccaa gcgcgggagc gaggtactga cggaaaatct
    13321 tcagatcaat ttcggtgtaa caatggttaa tgacgctctg ttctttaatg aagtagcaag
    13381 ggaacgtagc gcgggagatg atgtattctg agccgcgatg cacagtcagg cgtggaatat
    13441 cggcggtgcc ttttaacacc agatccagcc ggtcttcata ggggaagcgt gaagaatctt
    13501 ctttgactaa aaacagatgc aaccagtcgc actgtgccgc agcttgttga atcagataac
    13561 ggtgaccatt cgtaaaggga ttggcgttca tcacaatgca gccaatcttg ttccctggat
    13621 gacgaaattt tttcagcgat tcggcatagc gtttcagtcg cgtggcgctg ttttccatca
    13681 gcaccatcac gccgggtacg caggtcagcg tggaaaaacc gcactggcgg aacagcgcct
    13741 cgtattcggt ttt
//
LOCUS       DADLSB010000103        13639 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.103, whole genome shotgun sequence.
ACCESSION   DADLSB010000103 DADLSB010000000
VERSION     DADLSB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.103"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..739)
                     /locus_tag="JN331_002856"
     CDS             complement(<1..739)
                     /locus_tag="JN331_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7260959.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPISFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTI"
     gene            complement(932..2122)
                     /gene="glpC"
                     /locus_tag="JN331_002857"
     CDS             complement(932..2122)
                     /gene="glpC"
                     /locus_tag="JN331_002857"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY7260960.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(2119..3378)
                     /gene="glpB"
                     /locus_tag="JN331_002858"
     CDS             complement(2119..3378)
                     /gene="glpB"
                     /locus_tag="JN331_002858"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY7260961.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRVTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFAPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(3368..4996)
                     /gene="glpA"
                     /locus_tag="JN331_002859"
     CDS             complement(3368..4996)
                     /gene="glpA"
                     /locus_tag="JN331_002859"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY7260962.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGGRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            5269..6627
                     /gene="glpT"
                     /locus_tag="JN331_002860"
     CDS             5269..6627
                     /gene="glpT"
                     /locus_tag="JN331_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY7260963.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            6632..7708
                     /gene="glpQ"
                     /locus_tag="JN331_002861"
     CDS             6632..7708
                     /gene="glpQ"
                     /locus_tag="JN331_002861"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7260964.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7750..7992)
                     /locus_tag="JN331_002862"
                     /pseudo
     CDS             complement(7750..7992)
                     /locus_tag="JN331_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8154..8810
                     /gene="inaA"
                     /locus_tag="JN331_002863"
     CDS             8154..8810
                     /gene="inaA"
                     /locus_tag="JN331_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY7260965.1"
                     /translation="MTGDSSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGK
                     KLYVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRA
                     LLVTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRH
                     IYVKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM
                     "
     gene            complement(8864..9118)
                     /gene="yfaE"
                     /locus_tag="JN331_002864"
     CDS             complement(8864..9118)
                     /gene="yfaE"
                     /locus_tag="JN331_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY7260966.1"
                     /translation="MARVTLRITGTQLQCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(9118..10248)
                     /gene="nrdB"
                     /locus_tag="JN331_002865"
     CDS             complement(9118..10248)
                     /gene="nrdB"
                     /locus_tag="JN331_002865"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY7260967.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHQTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(10482..12767)
                     /gene="nrdA"
                     /locus_tag="JN331_002866"
     CDS             complement(10482..12767)
                     /gene="nrdA"
                     /locus_tag="JN331_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY7260968.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         3235 a         3338 c         3642 g         3424 t
ORIGIN      
        1 caatagtcat taatctctcc ttttgtagtg gtgaacgttc ggcaatctcc tgaataaaac
       61 gggtgaaacg ggaggtatcg ccgcattgca gcagataatt aaacagtgtt tgtagctggc
      121 tgtcattagt gaagcctcta accaaaagcg tggtgatcct gtcgaccatg ccgattaaat
      181 cgtggtcgcg aatatgcttt tgaatcagtt ccagcagagc tatacgccga tgttgcatga
      241 tctcatcgtc aggtacgatg gtgatatcca ccaacggaaa agcttcggtg tacaactgcc
      301 gggcaagttg cggatcgtca aactcatcca gccagttaag tgagtacggg tagggcgatg
      361 tttcgccatg ataaaacagc aatggcacca ccagcggaac tctgtcatag cctttatcta
      421 ggtgacgctg catggcggca gtggcatagc gcattggccg aaaagccata ttcttttctg
      481 cagagctttg atgttcaatc acgcagtaga tatagccgtc accgtcgctg gtttccacgg
      541 accacaaaac atccgagtag taagcgcgta aacttttttc aataaaacta atgggttcca
      601 ggcgtaaggt ttgcaggttg caaagcttgc gcagtggttc tggtaaatgt atttcgagaa
      661 aatcccgtgc ggtttcgggt gtgaatataa aggttttaaa taccgcatca tgcggggagg
      721 aggttgttga ttcggtcatg gttgtccgtc acctgatatt tatgatgacg cgacattacg
      781 gttatcccag gcctttgaca tctgccagtt tcagcctttg cgagacgttt tcagaggaat
      841 tttcactgcg ttgcaacgca tacattgggc acggaatcgt gtacgcaaaa atgcccggta
      901 accacatgct taccgggcat cagaagggag tttaagccag cgcctgggcc agtagcgtaa
      961 tcggatgttc gcagcgaaga cttgtggaca tctcaatctg ccatttacag gtttcgcagt
     1021 cggtgaccac cagatctgcg ccgctttctt ctatctggcg gaacagtggt gcgccgatgg
     1081 cttgtgaggt ggggtagttc tcttttttga aaccgtaagt acccgcaata ccgcagcact
     1141 gggaatccag caccgttaac tcaagccccg ggatgttacg caacagctcc agggtgtaga
     1201 gcgtccagcc cattttttcc atatggcacg gagtgtgata aaccactttc agcggcagcg
     1261 gtttcagcgg taacgttttg ccttcgtcca gcttgcgcca cagccagcgg gttgccagtt
     1321 cgatatgatc gcgcaagcct ttgttgtcga cattcagcac ttccgggtat tcgtcgcgca
     1381 gggcaaatgt acaggttgag gaggtggcaa tcaccggaat gccttttact cccacagctt
     1441 cgcggatcga ctctacattc gtaattgcct gtttgcgtgc tttatcggta aagccgttgg
     1501 cgattagcgg tacgccgcag catttttctt tgctgagcag ttgtacaccg gtacccattg
     1561 cgttgagcac tttaattaaa tctttaccta actgcggatg gttgtagtta acgaagcagc
     1621 cgtgaaagaa agcgacctgg tctttatatt gtgcttgctg agccgctatg ctgcgatacc
     1681 agcgacggaa cgtgccgaag gagtatttcg gtagcgtgcg acgatgatcg atttttaacg
     1741 ccgcatcaag cagctgccgc accggtttca gcgaggtagc ggtgttgacg attggtgcga
     1801 acggcgtgga aacgctaccc atcaggtcgg tatgactcaa cacaaaatta cgcagcgacg
     1861 ggcgcgtggt gtcatatttc gcccgcgcgc gctggataat atcgccaatc ttcacatcgg
     1921 acgggcaggc gacttcacaa cgtttgcagt tgatgcaata tttcagcgcc tcgtcataca
     1981 gtgcgccatc tttcaaacgc agacgctcgc catccggccc ggcttgtttt ggccctggat
     2041 aaccgggatt caccctgctc accgggcagg cggtggtgca gacggtgcac ttaatgcagt
     2101 tttcgaagct ggtgtcgttc attgttggcc tcctgcgcgt tgggcaatct gttgagcggc
     2161 atgtaaagca ctgacggcac aaacaccgcc gccgcatccc tgggcgatgg gagcaaatcc
     2221 gcccagcacc gaaccgatgg caaacaggtt ctcaatggtt tgccctgcct gcgacgggcg
     2281 tagcgtctca tcagtggtta caccgaactg ctgccacggt tgcggcgcaa aaaaatcgcc
     2341 cttataccat tcaccccgcg tggcggtttg tagcacatca aggccgagaa tcggctcgcg
     2401 aatgccgtta cgttccgcta ccagtccgcc actaaagaaa ctgccgctgg cgagaaccgc
     2461 gaaacgtgga cgtagcggaa tatcggcgtg attgcgggtc cagatttcat tcactacgcc
     2521 atttttacag gtcacttttt tcacttcatc gcccggcatc cacacgccac cctggcgcac
     2581 aaactggcgc tgtaactggt tttgcagacg aatgcccagc acggaaggcg gcagcgttgg
     2641 caaaagcatc agtgaacaag gtagtttttc attcaaccaa cgccacagtt tgtcatcggc
     2701 caggccgaag caggcgggca tcaggatcat ttcgcaggta ttggcgacag gaataagcgc
     2761 atcaagtaac agcggccagt tttcttcatt atcaaggaaa cgggcgatat tcaccgcgcg
     2821 aaattcggtg gcgttattgc gcaggacatc cagttccggc agctctattt ctgcggtttc
     2881 aaccgaaaga tccagttcgc gcaacgaagc cgccgcaaga tgcgcctgga aatccatcag
     2941 cccgcttatc cccactacac atatcttctt cgcgggtagc ggccagacgg gaacttctgg
     3001 cgaacttaac caggttacac gcaaagtgcc gagcggcgta atccgctggt gagccagttc
     3061 tacgctgccc tgcaattgcg caccgctttc agcgattaat gcctgcgcct ggcaagcgag
     3121 atcgagcacg cgttgtggcc cgagaagaga gtaaggatgt gcaggtgcct gctgacgcaa
     3181 agattccagt ccactgtgaa tgtctgccac gggttgacca tctggcagat ggctcagcaa
     3241 atccagcgaa ccggatgaga aatgcaacgc gctttgacca cgcgtgacaa tggcacagcg
     3301 caggccgtgt ttttgcagtt gcaggccaca gagtaatccg gcgaggccgc cgcccataat
     3361 gacagtatca aagcgcatct ttctgctcct tctccagacc acacagtccc tgataaaccc
     3421 agcgggtaaa ttcgctttcg cgcagtgcgt ctccccaggc gatgggttgc acgcctttcc
     3481 agcgttcgtt aaggaaggtg gaaagttgct cgatagattg cgcggacgtc gtgacgttaa
     3541 accgttgcag cagtccggca gcgcggcagg cgcagagttc gccctggcag gtgcccatcc
     3601 ccacacgggt acgacggcgt aaatccagca ggctattaac gtttaaattt tctaccgcgt
     3661 actgcacttc acccgcagtt accgcttcgc actcacatac caggctacgg tgcagacggc
     3721 cttcgctcag ccaggcaggc gtgcgatcgc catgacgata aaccgcagaa ccgcgcagcg
     3781 gggcaggcag ggagatgact ttacgcaagg taacttcagc gggttcttgt gaaccaggca
     3841 gtgccagatc ggcagtcgta caggggcgcg tgttgcccag tttgcggcat accgcgtcgg
     3901 tagcccattc agccatcagc cgataggtca tcagtttgcc accggtgatg gtgataaatc
     3961 cgtccagacc atcgcgttca gcatggtcga gcagcacgat gccacggctg acgttacgtc
     4021 cgctcgggtc gtcatcgctg gcaaccagcg ggcgcacgcc agaataggcc cgcaaaatgc
     4081 gcgttttcgc catcacgggg gccagttttt ccccttcacg cagcagaata tcaacctctt
     4141 ctgccgtcac tcgattatcg tcaatctcgt tgtaatcaat acgtaaagag gtggtaccaa
     4201 tcagcgaaat ggtatcgcca ggcaccagaa tatcggcgtc ggaaggttta cggcagcggt
     4261 tgatcacatg ctggttaatg cggtgatcca tgatcagcag cgatcctttc gccgggaaca
     4321 tgcgaatgcg cagatcggca tattcggcaa tgtgttgccc ccagatccca gcggcattaa
     4381 ccacgacagg tgcatgaagg gcctgagttt cgccggtgag atggttacgt acacgaccac
     4441 cgcacaccgt cgcgccttca cgaatcagcc ccgtgacttc atgagcggta aggataacgg
     4501 caccgtgttc tttggcatcc agcatgtttg ctgcggtcag acgaaatgga tcaacggtgc
     4561 catccggaac tttcaccgcg ccaatcagtg ccgggttaac ggcaggttcg ataatgcgcg
     4621 cttgctgcgg gtctatagct tctgcgctga tccctgcttc ttcgcaggcg cgaataaaag
     4681 tggcctggaa tgagaggtca tcttccggca gggtgataaa caggccgttg gttggttcaa
     4741 cgcagtgacg tgcaatgcgt ttcaggatct ggttttcact aatgcattcg cgggccgatt
     4801 ccgcatcggt tactgcatag cgcgcaccgc tgtgcagcag gccgtggtta cgcccggtgg
     4861 caccggttgc gatgtcgtgg cgctcaacca aaatcacgcg cagcccgcgc agggcacagt
     4921 cgcgggcaat cccggctccc gttgcgccgc cgccaatgat aatcacgtca cttgattgcg
     4981 agtcgcgagt tttcattgtt atccctctga agttcgtttt ttaccattta gccatagtaa
     5041 aaacataaat tgtttgattt cgcgcatatt cgctcataat tcgaaagtga aacgtgattt
     5101 catgcgtcat tttgaacatt ttgtaaatct tatttaataa tgtgtgcggc aattcacatt
     5161 taatttatga atgttttctt aacatcgcgg caactcaaga aacggcaggt tctctcactg
     5221 aatcaggctg ttaatcataa ataagaccac gggccacgga ggctatcaat gttgagtatt
     5281 tttaaaccag cgccacacaa agcgcgctta cctgccgcgg agatcgatcc gacttatcgt
     5341 cgattgcgct ggcaaatttt cctggggata ttctttggct atgcggctta ctatttggtt
     5401 cgtaagaact ttgcgcttgc tatgccttat ctggttgagc agggattctc acgcggtgat
     5461 ttaggttttg ccctttcggg gatctcgatt gcttatggat tttcgaaatt catcatgggt
     5521 tcggtatcgg atcgctcgaa tccgcgcgtt ttcctgcccg caggtttgat tctggcggcg
     5581 gcagtgatgt tgtttatggg ctttgtgcca tgggcgacgt cgagcattgc ggtgatgttt
     5641 gtactgttgt tcctctgcgg ttggttccag gggatggggt ggccgccgtg tggtcgtact
     5701 atggtgcact ggtggtcgca gaaagaacgt ggcggcattg tgtcagtgtg gaactgtgcg
     5761 cacaacgtcg gtggtggtat tccgccgctg ctgttcctgc tggggatggc ctggttcaat
     5821 gactggcatg cggcgctcta tatgcctgct ttctgcgcca ttctggtggc attattcgcc
     5881 tttgcgatga tgcgcgatac cccgcaatcc tgtggcttgc cgccgatcga agagtacaaa
     5941 aatgattatc cggacgacta taacgaaaaa gcggaacagg agctgaccgc gaagcaaatc
     6001 ttcatgcagt acgtgctgcc gaacaaactg ctgtggtaca tcgccatcgc caacgtgttc
     6061 gtttatctgc tgcgttacgg catcctcgac tggtcaccga cttatctgaa agaggttaag
     6121 catttcgcgc tggataaatc ctcctgggcc tacttcttct atgagtatgc gggtattccg
     6181 ggcaccctgc tgtgcggctg gatgtcggac aaagtcttcc gtggtaaccg tggggcaacc
     6241 ggcgtcttct ttatgacact ggtgaccatc gcgactatcg tttactggat gaacccggca
     6301 ggtaacccaa ccgtcgatat gatttgtatg attgttatcg gcttcctgat ctacggtcct
     6361 gtgatgctga tcggtctgca tgcgctggaa ctggcaccga aaaaagcggc aggtacggca
     6421 gcgggcttta ccgggctgtt tggttacctg ggcggttcgg tggcggcgag cgcgattgtt
     6481 ggctacaccg tggacttctt cggctgggat ggcggcttta tggtaatgat tggcggcagc
     6541 attctggcgg ttatcttgtt gattgttgtg atgattggcg aaaaacgtcg ccatgaacaa
     6601 ttactgcaaa aacgcaacgg aggctaatgg catgaaattg aagctgaaaa accttagcat
     6661 ggcgatcatg atgagcacta tagtcatggg aagcagtgca atggcggcgg acagcaacga
     6721 aaaaatagtc atcgcccatc gcggtgccag tggatatttg ccggagcata cgctgccagc
     6781 aaaagcgatg gcgtatgcgc agggagcgga ttatctggaa caggatttgg tgatgaccaa
     6841 agacgaccat ctggttgttc tgcatgacca ttatctcgat cgtgttactg atgttgccga
     6901 tcgtttcccg gatcgggcgc gcaaagacgg tcgttactac gcgatagatt tcacgctgga
     6961 tgaaattaag tcgctgaaat ttaccgaagg tttcgatatt gaaaacggta aaaaagtaca
     7021 gacttatccg gggcgtttcc caatgggtaa gtccgacttc cgggtgcaca cctttgaaga
     7081 agagattgaa tttgttcagg ggttaaatca ctctaccggg aaaaatatcg gtatctatcc
     7141 agaaatcaaa gcgccgtggt tccatcatca ggaagggaag gatattgcgg caaaaacgct
     7201 ggaagtgctg aagaaatatg gttacaccgg taaagacgat aaagtttatt tgcaatgttt
     7261 tgatgctgat gagctgaagc gtattaagaa tgagctggaa cccaaaatgg gcatggagct
     7321 caatctggta cagctgattg cctataccga ctggaatgaa acgcagcaga aacagccgga
     7381 tggaagctgg gttaattaca actacgactg gatgtttaag ccgggtgcca tgaaacaggt
     7441 ggcggaatat gcagatggta ttggtccgga ttaccatatg ttgattgagg agacatcgca
     7501 gccgggtaat atcaaactca ctggcatggt gcaagatgct cagcagaata aactggtagt
     7561 gcatccttat accgtgcggt cagataaact gcctgaatac actcctgatg tgaatcagtt
     7621 atatgatgct ctgtataaca aagcgggtgt aaatgggctg tttactgatt tccctgataa
     7681 ggcagtaaaa tttcttaata aagagtaatg ttactgcagg atggcgttca tgcgccatcc
     7741 tgtctcatat caaatttttc caatcgccac taattgttcc atgcacattt gtagtaaaaa
     7801 cagtggactg ccttctaccg gcttttcttt agtcagcaac gaaatctgtc gatagaaggt
     7861 tggttcgtga ggaacggaac acaattcacc tgcaatgctt ttcagcgtta actctggttg
     7921 taaggcgata cctaatccct gtcgaataaa attcatcgct gtatccgact gattaaattc
     7981 acattttatc tgctgggcgc actattagtg gtagtggctg gtggaatcta tgcgctgcaa
     8041 atgaagggca aaagcggtgt cacacctgta gcggcaaaaa aaattcatta atacgacacg
     8101 tttcattaag attttcctca ggtaaccacc tatagtcatt gtcgttaata aaaatgacag
     8161 gcgatagttc agcaaagtac gacgaattta atcactggtg ggcaacagag ggcgactggg
     8221 ttgaagagcc taactatcgc cgtaatggta tgagcggggt gcaatgcgtc gagcgcaacg
     8281 gcaaaaagct gtatgtaaag cgcatgacgc atcatctgtt tcattcagta cgttatccgt
     8341 tcggccgacc aacgattgtc cgtgaggttg cggtaattaa agaactggaa cgggccggtg
     8401 tcatcgtgcc gaagatcgtt tttggtgaag cggtgaaaat tgagggtgaa tggcgcgcgt
     8461 tgctggtgac tgaagatatg gcggggttca tcagcattgc tgactggtat gcccggcatg
     8521 cagtatcgcc ttattctgac gaagtacggc aagccatgtt gaaagcagtg gcactggcgt
     8581 ttaagaaaat gcatagcgtt aatcgtcagc atggctgttg ctatgttcgc catatctatg
     8641 tgaaaacaga aggtaaggca gaagctggtt ttcttgatct ggagaaaagt cgtcgccgct
     8701 tgcgtcgcga taaagcgatt aatcatgact tccgccagct ggaaaaatat ctggagccga
     8761 tccctaaagc ggactgggag caggtgaaag cgtattacta cgccatgtaa tcgttgtagg
     8821 ccagataata cgcgtaagcg tcgtatctgg cgctagtacc cgatcacaac tcgatttcaa
     8881 tatcgccttt tgcccggcaa caacagggca aaatttcccc cggctgaata aaggctaacg
     8941 gttcggcaat ccagtccacc tgacctgcga ccagtcgggt gcgacaggag ccgcagtaac
     9001 cttcgcggca ctggtactca accgccacat tgtgggattc cagcgccgcc agaagggaag
     9061 ggtgttcatc ctggcactgc agttgtgtgc cagtgatgcg cagggtaacg cgggccatca
     9121 gagctggaag ttactcaaat cgtcggtgtc cacttccgcg tcaatctgcc cgaccagata
     9181 agaactgact tccacttcct gcggagcaac ctgcacgtta tcagacacca gccaggtgtt
     9241 gatccacggg atcgggttgg agcgcgtctg gaacggcaga tccaaaccga ctgcctgcat
     9301 gcggatattg gtgatgtatt caacgtactg gcagagaata tctttattca gaccaatcat
     9361 cgaaccgtca cggaacagat aatccgccca gtctttctct tgctgtgccg cctggacaaa
     9421 caggtcatag cactcctgct tacattcttc ggcaatttcc gccatctccg gatcatccgc
     9481 gccgctgcgc agcagattca gcatatgctg agtaccggtc tggtgcaggg cttcatcgcg
     9541 ggcaatcagg cgaataatct tggcgttacc ttccatcagt tcgcgttctg caaaggcaaa
     9601 ggaacaagca aagctgacgt agaagcggat cgcttccagc gcgttaacgc tcatcaggca
     9661 gagatacagt tttttcttca gctcgcgcag gctaacggtt acggttttac cgttaacggt
     9721 gtgggtgcct tcgcccagaa ggtgccagta gctggtcatt tcgatcagtt catcgtaata
     9781 gctggagatc ccttccgcac gtttctggat ctgctcgttg gtgacgatat cgtcaaacac
     9841 aacagacgga tcgttaacga tattacgaat gatatgagta taggaacggg aatgaatcgt
     9901 ttctgagaac gcccaggttt cgacccaggt ttccagttcc ggaatagaaa taagcggcaa
     9961 tagcgccacg ttcgggctac gaccctgaat ggaatccagc agcgtctgat atttcaggtt
    10021 gctgataaag atgtgttttt cgtgctccgg cagcgcctgg taatctatac ggtcgcggga
    10081 gacgtcaact tcttccggac gccagaagaa agagagctgc ttttcgatca gcttttcgaa
    10141 gatgtcatat ttttgctgat cgtagcgagc cacgttgacc ggctgaccaa agaacatcgg
    10201 ttctttgagc tgatcatttt tcgtctgtga aaaggtggta tatgccatga gtgtgtcctg
    10261 ttgggagttt aatgccggat aaggcatttt acgccgcatc ctgtgcctga tgcgacgctg
    10321 gcgcgtctta tcaggcctac aaatccgagc cgtaggccgg ataaggcgtt cacgctgcat
    10381 ccggcacccg gagcctgatg cgacgctggc gcgtcttatc aggcctacaa accgagccgt
    10441 aggccggata aggcgtttac gccgcatccg gcatctcaat atcagatctt acatgcgccg
    10501 ctttcgcagc catcgtcctg gattgacggt accagatcgt cctgcgcgtc ttcagcgccg
    10561 tcacgggtgt tctgataata cagcgttttg accccgaatt tgtaggcggt gagcaggtct
    10621 ttcagcaact gctgcatcgg cacttttcct gacgggaaac gtgacggatc gtagttggtg
    10681 ttagcagaga tcgactgatc gataaatttc tgcatgatac ctaccagttg caggtagcca
    10741 tcgttacccg gcatttccca cagcagctca taggcgtcgt gcaggtgctc gtagtccggc
    10801 accacctggc gcaaaatacc gtctttcgac gctttgatgc tgacgtaacc gcgcggcggt
    10861 tcaataccgt tagtggcgtt agagatctgc gaagaggtct cggacggcat cagggcagaa
    10921 agcgtggagt tacgcaggcc gtgcgttttg attgattcac gcaatgcttc ccagtcgtaa
    10981 tgcagcggct cattagcgat ggcatccaga tctttcttat aggtatcgat cggcaggatc
    11041 cctttcgcgt aagtggtttc gttaaaccac gggcacgcgc cttgctcttt agccagctca
    11101 ttagacgctt tcagcaggta atactgaatc gcttcgaagg ttttatgcgt caggttgttg
    11161 gcgctgccgt cggagtagcg tttaccgtgc ttcgccagat agtaagcgaa gttaatcaca
    11221 ccaataccca gcgtacgacg ccccatcgct ccacgtttgg cggccgggat cgggtaatcc
    11281 tgataatcca gtagcgcgtc aagtgcacga accgccagaa tcgccagctc ttccagttca
    11341 tccaggctat taattgcgcc caggttgaaa gcagacagcg tacacagcgc gatttcaccg
    11401 ttctcgtcgt tgacgtcgtt cagcggtttg gtcggcaggg caatttccag gcacaggtta
    11461 gactggcgaa ctggcgcgat ggtcggatca aacggactat gggtattaca gtggtcaacg
    11521 ttctgaatat agatacgacc ggtagacgca cgttcctgca tcatcagcga gaacagctca
    11581 acggctttca cacgctgctt gcggatgctg tcgtctttct catatttggt atacaggcgt
    11641 tcaaactctt cctgatcggc gaaaaacgcg tcgtacagcc ccggtacgtc ggacgggctg
    11701 aacagggtga tatcttcacc tttcagcagg cgggtataca tcagtttgtt gatttgtacc
    11761 ccgtagtcca tatgacgcac gcggttgcct tcaaccccac ggttgttttt caacaccagc
    11821 aggctttcca cttccagatg ccacatcggg tagaacagcg ttgccgcacc gccgcgcaca
    11881 ccgccctgag agcaggattt caccgctgtc tggaaatgtt tgtagaacgg gatacagccg
    11941 gtatggaacg cttcgccacc gcgaatcggg ctgcccagcg cacgaatacg tccggcgttg
    12001 atgccgatcc cggcacgctg ggaaacatat ttcacaatcg cgctggaggt ggcgttgatg
    12061 gaatccaggc tgtcaccgca ctcgatcagt acgcaggagc tgaactgacg agtcggggta
    12121 cgcacgccgg acatgattgg cgtcggcagc gaaattttaa atgtggaaac cgcgtcgtaa
    12181 aaacgcttca cgtattgcag gcgcgtttca cgcgggtagt tcgagaacag gcacgcggca
    12241 acaagaatat aaaggaactg ggcgctttcg tagatttcgc cggtcacgcg gttctgtacc
    12301 agatatttgc cttccagctg cttaacggca gcataagaga aggtcatatc acggtcgtga
    12361 tcgataaacg tgtccatctg cttgaactct tcttccgtgt agtcttccag cagatgatta
    12421 tcgtatttgc ccatctcgac cattttcacc acgtggtcgt acagcgcagg cggctcaaac
    12481 tggccgtagg cttttttacg caggtggaag atcgccaggc gcgcggcgag atactgataa
    12541 tccggcgcat cacgggagat caggtctgcg gcagccttga taatagtttc gtggatgtca
    12601 gaggtcttga taccgtcata aaactgaatg tgggagcgca gctcgacctg ggaaatcgaa
    12661 acgttatgca gtccttctgc cgcccaatcc agaacgcgat ggattttgtc gagattgatg
    12721 cgctctgtgc taccgtcgcg ctttgtcacc agcagattct gattcatgta tgtcgtacct
    12781 gtttttggaa ttctttccgc aatagtttca tgcagactac ctgtagtaag ggaagctgct
    12841 tagaacacta tatatagggg gtatgtttgg gattcactgc aagatagtgt gaaaatgacc
    12901 ctcttgcaag tgcataactt tgtggataac tcaggaagga aaaagtggct ttcgcgcacc
    12961 ttaggtcaga caaggtgtcc gggaaagtca atgggaagag aaaaatttgt taaaaataac
    13021 tgttcggcga tttcttgaac ggcagaatag gttggataaa ttgatataga tggtttatta
    13081 atctttttgc taatgattgc aaaaattgat ctaagcacga gtcatcacat aatctgatgt
    13141 atcagttgtg gtcaatatat aagcgaatgt aagcgattgt tgatgtcgaa atggttgttt
    13201 tctattcggt tattgttaaa tgcgaagcca ttttgttaat ttaccttata ttataagaaa
    13261 attattcatt actttaattt agtttaaata taactggtat cccttaaggt tattaacttg
    13321 aattatgacg cactgatatt attcatcaaa taataacaaa atagccattg catcgggttg
    13381 gacaatttac aaaagaaaga taacccatat tgtataatgc gtttaaatat gccgtcttat
    13441 ataaaatgat ggtcagatta atatgcggat tatctttcta cgcaaggagt atttatctct
    13501 actcccgtca atgattgcat ctcttttctc tgctaacggt gtcgcggcgg ccattgattt
    13561 atgccaggga tatgatatca aagcgagttg tcacgccagc aggcaaagcc tttcaggcat
    13621 tacgcaggac tggagtatc
//
LOCUS       DADLSB010000104        13374 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.104, whole genome shotgun sequence.
ACCESSION   DADLSB010000104 DADLSB010000000
VERSION     DADLSB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.104"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            357..1187
                     /locus_tag="JN331_002867"
     CDS             357..1187
                     /locus_tag="JN331_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7260969.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIDIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1328..2101)
                     /gene="uxuR"
                     /locus_tag="JN331_002868"
     CDS             complement(1328..2101)
                     /gene="uxuR"
                     /locus_tag="JN331_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY7260970.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2316..3776)
                     /locus_tag="JN331_002869"
     CDS             complement(2316..3776)
                     /locus_tag="JN331_002869"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY7260971.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3857..5041)
                     /gene="uxuA"
                     /locus_tag="JN331_002870"
     CDS             complement(3857..5041)
                     /gene="uxuA"
                     /locus_tag="JN331_002870"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY7260972.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRLGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5381..6724
                     /gene="gntP"
                     /locus_tag="JN331_002871"
     CDS             5381..6724
                     /gene="gntP"
                     /locus_tag="JN331_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY7260973.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMIYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGMLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6975..7877)
                     /gene="fimH"
                     /locus_tag="JN331_002872"
     CDS             complement(6975..7877)
                     /gene="fimH"
                     /locus_tag="JN331_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY7260974.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7897..8400)
                     /gene="fimG"
                     /locus_tag="JN331_002873"
     CDS             complement(7897..8400)
                     /gene="fimG"
                     /locus_tag="JN331_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY7260975.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8413..8943)
                     /gene="fimF"
                     /locus_tag="JN331_002874"
     CDS             complement(8413..8943)
                     /gene="fimF"
                     /locus_tag="JN331_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY7260976.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8953..11589)
                     /gene="fimD"
                     /locus_tag="JN331_002875"
                     /pseudo
     CDS             complement(8953..11589)
                     /gene="fimD"
                     /locus_tag="JN331_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
     gene            complement(11656..12381)
                     /gene="fimC"
                     /locus_tag="JN331_002876"
     CDS             complement(11656..12381)
                     /gene="fimC"
                     /locus_tag="JN331_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY7260977.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12418..12957)
                     /locus_tag="JN331_002877"
     CDS             complement(12418..12957)
                     /locus_tag="JN331_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7260978.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13022..>13374)
                     /locus_tag="JN331_002878"
     CDS             complement(13022..>13374)
                     /locus_tag="JN331_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7260979.1"
                     /translation="QAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAIDATRTDVLA
                     LQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARYYAIGEATP
                     GAANADATFKVQYQ"
BASE COUNT         3284 a         3432 c         3190 g         3468 t
ORIGIN      
        1 tttatttccg cataatcagc tatgtattat ttgaaacaat tagtttgtaa ataatataaa
       61 ttgataacat atcaaataca tcaaaaatac cattcacagg cattattaat atagacttca
      121 atatagcaac atcaaattcc aattagtaat aagatatgct tttaaattgc atgcaaataa
      181 ttttaaatga gcgttttttg atactttaga gtgtttccta ccctttaata tcactgtgta
      241 gagttgatat cactacaaca gaaacatgca tgtcatttgt ttacttctct ttttactaca
      301 gaaaaattta tctatttttc taaacatcag ttaaatactc ataaaaggta actattatgt
      361 ctatgccatt aagcaatgcc gctaataacc aattcaatac aaataatcac ttccttcatc
      421 atccaaaagt cgacagtgag ttaacacgtc gatatgaata tgcacgcctg gatacggaaa
      481 atatctatct tctgcctctt gctcgtggca ataaccataa ttacgatgga aaaagcgttg
      541 tcgaaattag aaaacttaat attggcaaac agtcctggcc atttaattat gtgacagata
      601 catgcagaga attcaatggt attacaacaa caggacgtat gctctatcga aacttgaaaa
      661 tcacctcagc tttagacgaa atatatggtg gtatatgtaa aaaggcgcat gctgctacag
      721 agttagcaga aggtttacgg ctaaatttat ttatgaagag ccctttcgat cctgttgagg
      781 actatacagt tcatgaaata acattaggcc ctggttgcaa tgttcctggt tatgccggaa
      841 ccacgatagg atatatttca acccttccag cttctcaggc aaaaagatgg accaatgagc
      901 agccaagaat tgatatctat attgatcaga taataactgt ctcaggggta gcaaattctt
      961 ccggattcgc ccttgccgca ttacttaatg ctaatattga gatgggaaat gacccaatca
     1021 ttgatataga agcctatcct ggtaccgcgg agatccattc aaaaatggga tataacgtta
     1081 tccccggtga tgaagatgcg ccactcaaac gcatgactct gcaaccgtca tctcttccag
     1141 aactgtttga attaaaaaat ggtgaatgga attatattgg taaatagcca ctgagaagaa
     1201 tgagtactaa tattcaggtt atacagatat ttaaacaaaa ccctgcaatt tacaaagtaa
     1261 tactcattgc gacttcttaa tttaagaaag cgcaaggaac gtttaccctt gcgcttatta
     1321 taataagtca ggcgtcgact ttatccagcg gccatgaatc aaagagatag ccgtcaaaat
     1381 aaatatcgtc aacgttcgag aattccagca gacgttgctt aacgttttcc agatgctgcc
     1441 acattgccag cttcgccgct cgggcatctt ttttgattaa cgcggcgagg atctgtttgt
     1501 gatcgcccaa ccactctttg cgatacaggc tgtcatccag atggctgtgc aactggatcc
     1561 acattggatt gttttcccgc cactgccagg actgacggaa taactccacc agcatgctgt
     1621 tatgcgttgc ttcagcaatg gcgagatgga actgcatgtc accgctttcg ctgctacccg
     1681 gcgcgctgga agccagttca cgctcttcca gttgcaatgc ctgacgcatt ttgacgatat
     1741 cttcgcgggt agcctgcagt gcggcaaact cggcgatgtt actttccagt aactgacgcg
     1801 cctgtaacag ctcaaaagga cccgcatcat tgcagacgtt ggcatccgga ctgtctgtgt
     1861 tgtggctgcc tgagctatca agcacataga taccggcacc ccggcgcact tccaccagcc
     1921 ctttgatctc cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca tcaagcattt
     1981 ctgcaatttc acgctccggc ggcagccgtt cgccaggatt gtacggcgtc tttatgatca
     2041 gatcgcggat catcgccccg acttcctggt aaggtctttg cgcagaggtg acagatttca
     2101 tggtgttgtg tccgttaggt gcggttaatc aaatttttgc cggatgcggc gtgaacgcct
     2161 tatccggcct acaacgggca attttacggc ccccgttaat ctcaaactat accattagca
     2221 ctggaagttg gcacgtacag gtgggcaaaa aaacgaaaac tggtcaacca aatatcagtt
     2281 tgattgacca gtaagtctgt agggtaatca gttagttaca gcgcagccac acactcgcgc
     2341 gcaccgcgtt cgcacagctg ctgatatgcc gccgtcaccg cgccaacaaa gtcggcattc
     2401 tgcggcagat catcagcaaa aataccgctc aggcccagca gcgctttcac gcggtctgcg
     2461 ccctgatact gcgcgttaat cttctggaac tcggccagca tcgggtcgac cacgtcaatg
     2521 gcattaccct gctcatccac gccctgggtg taacgcatcc agccagccac gcccagcgcc
     2581 aggtgacgcc agctgccgcc gttttgcagg tgcagacgca ccgggtccag tagacgctgc
     2641 ggtaacttct ggctgccgtc catcgcaatc tgccaggtac ggtgacgcag agacgggttg
     2701 ctgaaacgct cgatcagcag cgtcgcatag gcgttcaggt ccgtaccttc cggcatcgac
     2761 agcgttggcg cttgttcctg catcatcagg gcaaaggccg ctttgcgata atccgggtta
     2821 gtcatggtgt cggcaatggt ttcatagccg ccgaggtaac cgaggtacgc caggaaagag
     2881 tggctgccgt tcagcatacg cagcttcatc atttcgaacg gcacaacgtc tgcaacgaac
     2941 tgtgcgccca ctttatccca gtccgggcga ccattaacga agttatcttc aatcacccac
     3001 tgacggaacg gttcgcaggc aatggcgcac gggtcgtaaa cacccagctg gtcagcaatt
     3061 tcctgtaagg tttctggcgt cgccgccgga acgatgcggt caaccatggt gcacgggaag
     3121 gtgacgttct cttcaatcca tgccgctagc tgcgggtcac gcgcctgagc cagccccagt
     3181 accgcgacct tcgccacatg accgttttca cgcacgttat cgcaggacat caccgtaaat
     3241 gctttcagcc ctttttcacg acgcagacgc aaggcttcga cgatgtaacc aatcgcggac
     3301 ttcggcgcag tcgggttttc cagatcgtgc ttgatcagcg ggttattgag atccagttga
     3361 ccgcttgccg catcagcgca gtagcctttt tccgtgaccg ttagagagac aatcgccgtt
     3421 tgcggacgcg ccatcgcgtt gagaatacct tcgcagccgt cgatttccgg atgcagcgct
     3481 tctttcatcg aaccgataat tttcagctcg gtgctttctg cgcctttttc cgctacggtg
     3541 tacagcagtt gctgtttttt caggttttcg atcagcatgc ggtcgttgcc tggcatcagg
     3601 ttaacttcgc agatgcccca gtcgctgtcg gtgctttcca gcagatgatg ggtatacagc
     3661 gcctggtgcg cgcggtgaaa cgccccgcaa ccgagatgca caatgcgtga ttccagacga
     3721 gaatgatccc atgacgggcg ggcaaccggc agattgctgt caacaatagt agtcatcgta
     3781 aactccagaa tccgtcttca gagagtgacg gcaaggaaga aggaaccggc acggcgcgca
     3841 gccatgccgg tgggtattaa cggctaaaga aagcgcgctg gatcgccagt tcgacaccgc
     3901 gaacttcggc caggcctttc agacgaccaa ttgcggagta accagggttg gttttcttct
     3961 tcaggtcgtc cagcatctga tgaccgtggt ccggacgcat cgggatcagg tcttctttgc
     4021 cttccgcttt acgacggtgt tcttcttcaa caatcgcttt caccacttcg tacatatcaa
     4081 cgtcaccgtt caggtgcgcc gcttcgtgga aggttttcgg gttatcttca cgcatggtgg
     4141 agcgcagatg ggtgaagtaa atacgcggac cgaactgctt gatcatatca accagatcgt
     4201 tgtcagcacg cacgccgtag gaaccggtgc acatggtgaa accgttcgcc atgctgttta
     4261 cggtatcaac catccactgc atatcttcaa tggtggaaac aatgcgcggc aagccgagga
     4321 tcgggcgcgg cggatcgtcc gggtgaaccg ccatacgcac gccaacttct tcagcaactg
     4381 gaataatcgc tttcaggaag acggcaaagt tttcgcgcag tttggctttg tcgatatctt
     4441 tgtacagctc caggtgctta cggaactggt cgagggtata cccttcttcc gcgcccggca
     4501 gaccggcaat gatgttacgc gtcagacgcg ctttgtcttc gtcgctcata gtggcgaagc
     4561 gttcagctgc ctgagcaatt tcttcttcgg tgtaatccgc ttccgcgcct agacgtttca
     4621 ggatatgcat ttcgaatgca gcgaattcga tctggtcgaa gcgcagagct ttggagccgt
     4681 ctggcagcac gtattcgagg tcagtacggg tccagtcgag caccggcatg aagttgtagc
     4741 acacggtgcg aatgccacac tgcgccaggt tgcgcagagt ctgctgatag ttagcgatcc
     4801 actgctcata gttgccagtg tgggttttga tatcttcgtg aattggtacg ctttctacga
     4861 cagaccacac caggcctgcg tcttcaacga tcgccttgcg tttgaggatc tcttctacgg
     4921 accatacttc gccgttcggg atatggtgca gcgcggtaac cacgccagtt gcgcccgcct
     4981 gacggacatc ggctaaagaa accggatcgt ttgggccgta ccagcgccag gtctgttcca
     5041 tgatttttcc tctttcacat agtgggttcc acagcaaaga gacgcggaac cttggagttg
     5101 tttaggtatc cggatgaaca accagaccgc agccgtggcg ttgcatcgga tgagatggcg
     5161 tataagttct accttttggt aagacatgtc aacctgaatt ctgaaattgg ttaaccacat
     5221 cacaagaatt tcactctcat ggttgttgct gcaaattgca gcaaagggta atcgatggtt
     5281 attgatcaga ggatgtgaca ttcatcgcaa caatggttga ccaatttaca taacatgtcg
     5341 cccaaaataa caacggttca accaagtaag gtaaaacgtt atgcatgtgc ttaacattct
     5401 ctgggtggta ttcggcattg gcctgatgct gatactgaat ttgaagttca aaatcaattc
     5461 aatggtggct ttgttggtgg cggcgctgtc cgtcgggatg ctggcaggca tggatttgat
     5521 gtcgctgctg cacaccatga aagcgggctt cggcaacacg ctgggagaac tggctatcat
     5581 cgtggtgttc ggtgcggtca tcggtaaatt gatggtcgac tccggcgcgg ctcaccagat
     5641 agcacatacg ctgctggcgc gtctcggtct gcgctatgta cagctgtcgg tgattatcat
     5701 cggccttatt ttcggtctgg cgatgttcta tgaagtggcc tttatcatgt tagcgccgct
     5761 ggttattgtt attgccgccg aagctaaaat tccgttcctg aaactggcga tcccggcagt
     5821 agcagctgcc actaccgcac attcactgtt cccaccgcag ccgggtccgg tggcgctggt
     5881 gaatgcttat ggcgcggata tggggatgat ttatatctat ggcgtactgg tgacgatccc
     5941 aagtgtaatc tgcgcaggtc tgatcctgcc gaagttcctc ggcaatcttg agcgcccaac
     6001 gccatcattc ctgaaagcag atcaaccggt agatatgaat aatctgccct ctttcggcgt
     6061 ttcgattctg gtgccgctga tcccggcgat cattatgatc tctaccacca tcgccaatat
     6121 ctggctggta aaagataccc ctgcctggga agtggttaac tttattggtt cctcgccgat
     6181 tgcgatgttt attgcgatgg tggttgcatt cgtactcttt ggcaccgcgc gtggtcatga
     6241 catgcagtgg gtgatgaacg cttttgaaag cgcggtgaag agtattgcaa tggtgattct
     6301 gatcatcggt gcgggtggca tgctgaagca gaccatcatc gacaccggca ttggcgacac
     6361 catcggcatg ttgatgtccc acggcaatat ctcgccctac attatggcat ggctgatcac
     6421 tgtgctaatt cgtctggcga cgggtcaggg tgtcgtttcg gcgatgaccg ccgccgggat
     6481 tatcagtgct gcaatccttg atccagcaac cggtcagctg gttggcgtga atccggcgct
     6541 gctggtactg gcgacggctg cgggttccaa caccctcacc cacattaatg atgcatcttt
     6601 ctggttgttc aaaggttact ttgacctgtc agtaaaagac acgttgaaaa cctggggtct
     6661 gctggagctg gtcaactccg tggttgggct gattattgtg ctgattatta gcatggtagc
     6721 gtaaaagaac accagagcct gccaatggca ggctcagact gatgactaaa gtctaaaaat
     6781 gtgcccggac agtcccctcg cccctccggg gagagggtta gggtgagggg aacagaccag
     6841 taaagtgcgc tttgtttatg ccagatgcga cgctgacgcg tcttatctgg cctacaaagg
     6901 gctaacgtgc aggttttgta ggtctgatag cgtagcgcct caggtaccag cattagcaat
     6961 gcgctgtaat ttttttattg ataaacaaaa gtcacgccaa taatcgattg cacattccct
     7021 gcagtcacct gccctccggt acgtgcgtaa tttgccgtta atcccagact taccgccgaa
     7081 gttcctactg ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc
     7141 aactgtacgc cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg
     7201 cccgcatctg cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa
     7261 acggtaagag gaattggcac tgaaccaggg tagtccggca gagtaacggt gacatcacga
     7321 gcagaaacat cacaaccgcc agtgggcacc accacatcat tattggcgta aatattccac
     7381 acaaactgaa aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt
     7441 aatgaaccag ctttaatcgc caccccgccc gcactgctca caggcgtcaa ataaagcgcc
     7501 accggccacg gcttatccgt tctcgaatta taaacaaccc gcggcgtttc gctggtggtc
     7561 ggaaatggat agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg
     7621 ccgtaagccg agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta
     7681 tggcaaaaga tttgcgtcga aagatctacg accaggtttt gccccacatt cacgacaggc
     7741 gcaaggttta cataaacatt agcgctgcca ccgccaatag ggatagcggt accattggcg
     7801 gttttacagg cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg
     7861 gtaatagctc gtttcattac aattatctct tcgggttcag ctgtaggtat aggtgatgct
     7921 aatcactgcc tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg
     7981 taacgggaag tgcgctgatt gtgaggaatc atccacctga actgttttgg ttgcgccagt
     8041 attcaatgtg ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg
     8101 gtttttataa tatccggtgc tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt
     8161 tcccaccgga caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc
     8221 agacataaga ctgaaagaat aaagatcgcc gagatctacc gtggcattgg tggtggaaac
     8281 cgtacacggt ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat
     8341 cgtcgcactt gcgagcgcca acattgccgc caatacatac ccacgtttgc accatttcat
     8401 gagcatctcc agttactgat attcaagagt gaaggtagcc gtagcattga tatgccccgc
     8461 agtgacaggc acctgtgtcg ccattagccg ggcgtaaaaa ttcagcgtat ttggtttacc
     8521 cggcgtcagg gtcgtccacg aaatcgcaga cgatggagca ttaaggggta tttgattttg
     8581 ctgctcattc agaagctgta ttcccagtcc cgcagccgct gacaccgtat tttcaagtgc
     8641 aagcaggttg gcattgtggc tatctgcaac gccggtaaac ccaactttta cggcagaaac
     8701 ggcattacca cagggtgaca gcaaaatacg aaatggaacg acaggagtcg tcgcgccaat
     8761 gttgttaaat tgcttcgccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc
     8821 ggccacacta cagccgttat ctctgacata gccgcggata gtaatcgtgc tatccgcagc
     8881 caaaacgcga cttaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct
     8941 catcacgccc ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc
     9001 tggtggcagt tgataattgg cgacacagtg agcattttcc tcttctcccc atttcacctg
     9061 aacttttccc gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc
     9121 gctactctgg ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg
     9181 agttagcgtc atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc
     9241 cccacgagtg ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccaaggt
     9301 attggtatcc agcgccactc tattttcccg atattcagtg gcataaggca gcacggcata
     9361 accacgccag tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg
     9421 cgctttaaca agcaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg
     9481 agccaatacc ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta
     9541 accgatattg gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc
     9601 gctattacca tcgcctcccc cggcatagcc ggtttgcacg ctataactga ggttgttgtc
     9661 ttccagcaac gtaccgtata caccagccag attggtcatc cgaccgttga gatcgtgtga
     9721 catgctgtag ctggcactgg catgtcacca ctgagattta ctgtcagaac gcagccagtg
     9781 gctgaaagga atattgacgt taagcgctaa catctgatcc cgtccttttt gccaggcgtt
     9841 tttcgtcagg ctatagctga gcgtccagtt gatatcttcg aacgcagtat ttaatccagc
     9901 ctggaattgc tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata
     9961 cagtgttgat gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt
    10021 ataagcgagg ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt
    10081 ttcgatgttg tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct
    10141 ggtcgaataa cggtaaccca ctaactgaat attcgtgcct gattcattga gcgatttgtt
    10201 atagagaaaa cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc
    10261 ctgcgtcata tccacagaca gagcgcccag tgcccccatg tttttcccga taccgaaatt
    10321 aaaagcacga taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag
    10381 gccgtggagt aatgtactct ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg
    10441 gtattctcct gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga
    10501 cgaatagggt acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa
    10561 gtcaccacta ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt
    10621 actattataa atgccatatc cattttgttt aatagtgacc tgtgcagtac cacgagcaat
    10681 accgtggatc accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc
    10741 caattgtgcg ccgcgaaagt taataccatc gaaaatatcg ccctgggtat aaccatcacc
    10801 caacgtcagc cgggaacgta aaggaattat gtctcgctca atccaggtaa tgatatgctg
    10861 ccatttattt ttgctacctg atgaactgtc gctactgtta taactccagg tggtattgtc
    10921 gcgtaaacgc cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct
    10981 gttaccccca atccgattct gtacactatt tccgctgaaa ttataattga gcaatccggc
    11041 attaataccg ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa
    11101 tgcctgaggg atcgtcaggt tcagtcgctg ctgaccaaca tccagatgcg cagtagcgtc
    11161 ctggaccatt gtggttaatg gcacacaggc atcatccgcc agcagattca taccggcgac
    11221 agaagccgta ttcagcccca tactggcgag ttgcgcgcgt gtcaggcagg gaacaatccc
    11281 ttgttcactg tcgcccgtat taaatgtgac atcacgcgtt gccatataac cattattcaa
    11341 atagatatcg acgcgatacg tccctggcgg taattcttgc ccattttaaa aacgcgataa
    11401 atcggccaca gcctggggat catccgctaa aaagcgcaga ttaaaataga ggtcggcaga
    11461 tgacaaaggt gcctgtgcgg caaaagcaca ggcgacaacg agtcggacaa aaaaaccagc
    11521 caaacgatgc ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag
    11581 atatgacatt attcctccgg tttaaaatca cccggcagtc aattcttttt attcaggcga
    11641 aaaattcccc ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg cgccataatc
    11701 atttattgtt cggtaagtaa tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc
    11761 gcccattgga ggcaccaatg cattttcaag aacccgggtt ccggcattca actctgttac
    11821 cgtcaggtaa tagggtgtcg ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct
    11881 taatttttct gcggcctgat cgggtggcaa cgctaattta gccgggcgat agtacagttt
    11941 aatgcggctg ataattgcga gctgtagcgt attctcagtc aattttgatt tatccattga
    12001 cggaatcgct ttaacgttca tccagaataa actttcccgg tcctgtggca attggttatt
    12061 tgttgcatca agaatacgta aggtattctc tttttttccc ttcatcgcaa acagaggagg
    12121 cgtcacgata aaacgaccat cctttacacc atcggcattt tccacccatg attgaattaa
    12181 ataggtacta ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg
    12241 ataaattacg cgagtcgcac ctaaggccac tcctgcttca gcgcgtccgg caaccatcat
    12301 tgccatgaac atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac
    12361 attgacgttt ttattactca ctgtcctgtt cctgttatca cattatctac tgaacaatta
    12421 ctgataggtt aaagagaacc aggcctgggc attggcgatg ccgccagtaa cccgacgccc
    12481 ggtagcacga tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca
    12541 gtttgctgga ggacgattaa tcggtacgag gtttccttca tcatcaaaca acgctacacc
    12601 aatattggag gctatccctg gcccctctcc cacggagagc acatccggat ttttaccatc
    12661 cgcgacaccg tgaaacgcca cacccacacg ttcactcacc accgtgctac attcccgtaa
    12721 atgaataaca aaaggcaccg gtgcgctatc ttcccctgcc gcatgaaacc ggttactgct
    12781 gatttgcccc atattgaccg tcatttgttt atcaccggct tcaatccggc aagtttccgc
    12841 aataatgacg ccctgaaatt gcatatttcc gccgggcagc gtggtattcc atttatttcc
    12901 ggccagagca aacattggca gcaacgacgc taacagaaat aatctcttcc ttttcatcgt
    12961 tatttttatc gcacaagggt gggcatccct gcccgtaatg acgtccttga acctgggtag
    13021 gttattgata ctgaaccttg aaggttgcat ccgcattagc tgcacccggg gttgcctcgc
    13081 cgattgcata ataacgcgcc tggaacggaa tggtgttggt accgttattc agggttgttt
    13141 gtgcactaaa tgtcgcaccg tccagcgtca gagcattgcc tgttctgtcc aggatctgca
    13201 cgccaacgtt tgttgcacta cctgcagcag aactctgcag agccagtaca tcagtacgcg
    13261 tagcatcaat tgccgtacct aagaaggcaa cagcggcttt tgtggcaaca gtggtatcgc
    13321 aatcattcag ctgaatgtta aaaccaacgg cagagctggt tgctccagcc tgct
//
LOCUS       DADLSB010000105        13239 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.105, whole genome shotgun sequence.
ACCESSION   DADLSB010000105 DADLSB010000000
VERSION     DADLSB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.105"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..328)
                     /locus_tag="JN331_002879"
     CDS             complement(<1..328)
                     /locus_tag="JN331_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260980.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNVRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIATI
                     FEEYLME"
     gene            complement(645..1469)
                     /gene="iclR"
                     /locus_tag="JN331_002880"
     CDS             complement(645..1469)
                     /gene="iclR"
                     /locus_tag="JN331_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY7260981.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1669..5352
                     /gene="metH"
                     /locus_tag="JN331_002881"
     CDS             1669..5352
                     /gene="metH"
                     /locus_tag="JN331_002881"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY7260982.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKWIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYARDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGKDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5572..7203
                     /locus_tag="JN331_002882"
     CDS             5572..7203
                     /locus_tag="JN331_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY7260983.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQGLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7294..7971)
                     /gene="pepE"
                     /locus_tag="JN331_002883"
     CDS             complement(7294..7971)
                     /gene="pepE"
                     /locus_tag="JN331_002883"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY7260984.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFGVTQTWDD
                     YTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGLLAPI
                     TDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTNALPE
                     GHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGEEAVP
                     LEAGHRF"
     gene            complement(8353..9582)
                     /locus_tag="JN331_002884"
     CDS             complement(8353..9582)
                     /locus_tag="JN331_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7260985.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            complement(9634..10422)
                     /locus_tag="JN331_002885"
     CDS             complement(9634..10422)
                     /locus_tag="JN331_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY7260986.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            complement(10433..11230)
                     /locus_tag="JN331_002886"
     CDS             complement(10433..11230)
                     /locus_tag="JN331_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7260987.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            complement(11296..11790)
                     /locus_tag="JN331_002887"
                     /pseudo
     CDS             complement(11296..11790)
                     /locus_tag="JN331_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(11790..12197)
                     /locus_tag="JN331_002888"
     CDS             complement(11790..12197)
                     /locus_tag="JN331_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY7260988.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            complement(12207..13013)
                     /locus_tag="JN331_002889"
     CDS             complement(12207..13013)
                     /locus_tag="JN331_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7260989.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(13083..>13239)
                     /locus_tag="JN331_002890"
     CDS             complement(13083..>13239)
                     /locus_tag="JN331_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7260990.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
BASE COUNT         3163 a         3420 c         3482 g         3174 t
ORIGIN      
        1 tttccattag gtattcttca aatattgtag caatggtttg agtgagtgtc gtgttttcgt
       61 ttttattaat tatcgtaatt tctttcttat cagcttgtaa gacggacgtg gaaaatttaa
      121 tgacaaaatc cttgttgcta aagatatgac gaacattttg tctacagttc tctgagaagc
      181 tttttaatag aggcgtcgcc agctccttgc cagaaaattt atcctcgagt tctttataaa
      241 acaattcact cagggtttgg tgttcatttg tctgggctgt attattaata tttgcagaga
      301 aagaactacg aggaatacga gtaatcattt aaatatctca ttgtttattg atgtcttgaa
      361 ttttaactat cagaacagtg aaaaaattta atatgattac aactaaaaaa atatcataaa
      421 tcgctcaatc tcataatgca gccgtaaaag tttcggtgga atgagatctt gcgattttgt
      481 taataattaa gtggtttgat gtttaaaaat aatgctgagt taataaaagt gtgtgaagtg
      541 tatgacatta agttattttg gcgtcaatgc gattaacaga cacccttatt ctattgccac
      601 tcaggtatga tgggcagaat attgcctctg cccgccagaa aaagtcagcg cattccaccg
      661 tacgccagcg tcacttcctt cgccgcttta atcaccatcg cgccaaactc ggtcacgcga
      721 tcatcggtaa tacgtgaaat cggtccggaa atagaaattg cggcaaacgg ttcgcggtgc
      781 tcatcgaaaa tacacgctgc aaggcaacgt agccccagcg catgttcctc atcgtcaaat
      841 gaataacccc gtttgcgcgt ttgggcgaga tcttctttta aatgcacagg agacaccaga
      901 gttgcgtggg tataggcatg taaccctttg cggtgcagca gcttcgtcac ctgttcttcg
      961 ctcagttggg ctaaaaaggc tttacccgca ccggaagcgt gcatcggcaa tttaccgccg
     1021 ataggcgcgg acattcgcat caggtgggta cactgtacct ggtcgataat aatcgcttcg
     1081 tgatcgcttt gatcaagcac cgccatattg accgtttcgc cagactcttc cattagattg
     1141 cgcaggatag ggtgaacaat tgctaacaaa ttacggctct ggagaaagct gctgccgacc
     1201 ataaaggcat gtgcgccgat tgcccagtgt cccagttcgc cgacctgacg cacgaaaccc
     1261 tgctgttgca tcgtggttag caggcggtgg gtcgtggaat tgggtaaccc ggcttgttgc
     1321 gccagctctg tgagtgccac gctgccattg gattcggcaa tccactccag taatttcagg
     1381 ccacgcgtta aagactgaac ctgtccagtc gctggtgcgg tggcaacggc gggttttctg
     1441 ccgcgtttcg cgggaatggg tgcgaccatg acagcctcct ttttctgtat cgtggaaatt
     1501 attttcattt ttattgttag ctaatgcaat agttgctgaa ctgatccgat gagttaatgt
     1561 tgaacaaatc tcatgttgcg tggtggtcgc ttttaccaca gatgcgttta tgccagtatg
     1621 gtttgttgaa tttttattaa atctgggttg agcgtgtcgg gagcaagtgt gagcagcaaa
     1681 gtggaacaac tgcgtgcgca gttaaatgaa cgtattctgg tgttggacgg cggtatgggc
     1741 accatgatcc agagctatcg actgaacgaa gccgattttc gtggtgaacg ctttgccgac
     1801 tggccatgcg acctcaaagg caacaacgac ctgctggtac tcagtaaacc ggaagtgatc
     1861 gccgctatcc acaacgccta ctttgaagcg ggcgcggata tcatcgaaac caacaccttc
     1921 aactccacga ccattgcgat ggcggattac cagatggaat ccctgtcggc ggaaatcaac
     1981 tttgcggcgg cgaaactggc gcgcgcctgt gctgacgagt ggaccgcgcg cacgccggag
     2041 aaaccgcgct acgttgccgg tgttctcggc ccaaccaacc gcacggcgtc tatttctccg
     2101 gacgtcaacg atccggcatt ccgcaacatc acttttgacc agctggtggc ggcctatcgc
     2161 gaatccacca aagcgctggt ggaaggtggt gcggatctga tcctgattga aaccgttttc
     2221 gacaccctta acgccaaagc ggcggtcttt gcggtgaaaa cggagtttga agcgctgggc
     2281 gttgagctgc cgattatgat ctccggcacc atcaccgacg cctccgggcg cacgctctcc
     2341 gggcagacca ccgaagcatt ttacaactca ttgcgccacg ccgaagcgct gacctttggc
     2401 ctgaactgtg cgctggggcc cgatgaactg cgccagtacg tgcaggaact atcacggatt
     2461 gcggaatgct acgtcaccgc ccacccgaac gccgggctgc ctaacgcctt tggtgaatac
     2521 gatctcgacg ccgacacgat ggcaaaacag atacgtgaat gggcgcaagc gggctttctc
     2581 aatatcgtcg gtggttgctg cgggaccacg ccgcaacata ttgcagcgat gagtcgtgca
     2641 gtagaaggat tagcgccgcg caaactgccg gaaattcccg tagcctgccg tttgtctggc
     2701 ctggagccgc tgaacattgg cgacgatagc ctgtttgtga acgtgggtga acgcaccaac
     2761 gtcaccggct ctgctaagtt caagcgcctg atcaaagaag agaaatacag cgaggcgctg
     2821 gatgtggcgc gtcagcaggt ggaaaacggc gcgcagatta tcgatatcaa catggatgaa
     2881 gggatgctcg atgccgaagc ggcgatggtg cgttttctca atctgattgc cggtgaaccg
     2941 gatatcgctc gcgtgccgat tatgatcgac tcctcaaaat gggatgtcat tgaaaaaggt
     3001 ctgaagtgga tccagggcaa aggcattgtt aactctatct cgatgaaaga gggcgtcgac
     3061 gcctttatcc atcacgcgaa attgttgcgt cgctacggtg cggcagtggt ggtgatggcc
     3121 tttgacgaac agggacaggc cgatactcgc gcacggaaaa tcgagatttg ccgtcgggcg
     3181 tacaaaatcc tcaccgaaga ggttggcttc ccgccagaag atatcatctt cgacccgaac
     3241 atcttcgcgg tcgccaccgg tattgaagag cacaacaact acgcgcggga ctttatcggc
     3301 gcgtgtgaag acatcaaacg cgaactgccg cacgcgctga tttccggcgg cgtatctaac
     3361 gtttctttct cattccgtgg caaagatccg gtgcgcgaag ccattcacgc agtgttcctc
     3421 tactacgcta ttcgcaatgg catggatatg gggatcgtca acgccgggca actggcgatt
     3481 tacgacgacc tacccgctga actgcgcgac gcggtggaag atgtgattct taatcgtcgt
     3541 gacgatggca ctgagcgttt actggagctt gccgagaaat atcgcggcag taaagcggac
     3601 gacactgcta acgcccagca agcggagtgg cgctcgtggg acgtgaataa acgtctggaa
     3661 tactcgctgg tcaaaggcat aaccgagttt atcgagcagg atactgaaga agcccgccag
     3721 caggctacgc gcccgattga agtgattgaa ggcccgttga tggacggcat gaacgtggtc
     3781 ggcgatctgt ttggcgaagg caaaatgttc ctgccacagg tggtcaaatc ggcgcgcgtc
     3841 atgaaacagg cagtggcgta tctcgaaccg tttattgaag ccagcaaaga gcagggtaaa
     3901 accaacggca agatggtgat cgccaccgtg aaaggcgatg tccacgacat cggtaaaaat
     3961 atcgttggtg tggtgctgca atgtaacaac tacgaaattg tcgatctcgg cgttatggtg
     4021 cctgcggaaa aaattctccg tatcgccaaa gaagtgaatg ctgatttgat tggcctttcg
     4081 gggcttatca cgccgtcgct ggacgagatg gttaacgtgg cgaaagagat ggagcgtcag
     4141 ggcttcacca ttccgttact gattggcggc gcgaccacct caaaagcgca cacggcggtg
     4201 aaaatcgagc agaactacag cggccctacg gtgtatgtgc agaatgcctc gcgcaccgtt
     4261 ggtgtggtag cggcgctgct ttccgatact cagcgtgatg attttgtcgc tcgtacccgc
     4321 aaggagtacg aaaccgtgcg tattcagcac gggcgcaaga aaccacgcac accaccggtc
     4381 acgctggaag cggcgcgcga taacgatttc gcatttgact ggcaggctta cacgccgccg
     4441 gtggcgcacc gtctcggcgt gcaggaagtg gaagccagca tcgaaacgct gcgtaattac
     4501 atcgactgga caccgttctt tatgacctgg tcgctggccg ggaagtatcc gcgcattctg
     4561 gaagatgaag tggtgggcgt tgaggcgcag cggctgttta aagacgccaa cgacatgctg
     4621 gataaattaa gcgccgagaa aacgctgaat ccgcgtggcg tggtgggcct gttcccggca
     4681 aaccgtgtgg gcgatgacat tgaaatctac cgtgacgaaa cgcgcaccca tgtgatcaac
     4741 gtcagccacc atctgcgtca acagaccgaa aaaacgggat tcgctaacta ctgtctcgct
     4801 gacttcgttg cgccgaagct ttctggtaaa gcagattaca tcggcgcatt tgccgtgact
     4861 ggcgggctgg aagaggacgc actggctgat gcctttgaag cgcagcacga tgattacaac
     4921 aaaatcatgg tgaaagcgct tgccgaccgt ttagccgaag cctttgcgga gtatctccat
     4981 gagcgtgtgc gtaaagtcta ctggggctat gcgccgaacg agaacctcag caacgaagaa
     5041 ctgatccgcg aaaactacca gggcatccgt ccggcaccgg gctatccggc ctgcccggaa
     5101 catacggaaa aagccaccat ctgggagctg ctggaagtgg aaaaacatac tggcatgaaa
     5161 ctcacagaat ctttcgctat gtggcctggt gcatcggttt cgggttggta tttcagtcat
     5221 ccggacagca agtactacgc ggtggcgcaa attcagcgcg atcaggttga agactatgcc
     5281 cgccgtaaag gtatgagcgt ctccgatgtt gagcgctggc tggcaccgaa tctggggtat
     5341 gacgcggact gattcacaaa tctgtcactt ttccttacaa caaacagggc gctcaatgag
     5401 tgccctgtct ctttattaat atgaaacact tatactggaa acaggctgga ataatcttag
     5461 ccggaaaaac gaatgtcaaa aggcacagta aagatgccag tcagcgtgtt tccggtcctt
     5521 tggtcattca attgcttcag gaacatataa cgataaggag aacctgaact cgtgttaacg
     5581 ctgcttcacc tgctttctgc cgtcgccctg ctggtctggg ggactcatat tgttcgaacc
     5641 ggcgtaatgc gcgtcttcgg cgcgcgtttg cgtactgtcc ttagccggag cgtcgaaaaa
     5701 aagccgctcg ccttttgcgc ggggatcggc gttaccgcac tggtacagag cagtaatgcc
     5761 accaccatgc tggtgacctc gttcgtcgct cagggtctgg tagccctcgc accggctctg
     5821 gtcattgtgc tgggggcaga tgtcgggacg gcgctaatgg cgcgtattct caccttcgac
     5881 ttatcctggc tgtcaccgtt acttattttt atcggcgtga tttttttcct cggacgcaaa
     5941 cagtcacgcg ccgggcaact gggccgcgtc ggtattggtc ttgggctgat tttgctggcg
     6001 ctggagttga ttgtgcaggc cgtaacgccg atcacccagg caaacggcgt tcaggtgatc
     6061 tttgcctcgc tgaccggcga tattctgctg gatgcgctga ttggcgcgat gttcgccatt
     6121 atcagctact ccagccttgc tgctgtactg ctgaccgcga ctctgaccgc tgcaggcatt
     6181 atctccttcc ccgtggcgct ctgtctggtg attggagcta acctcggttc cggcctgctg
     6241 gcgatgctca acaacagtgc cgccaatgcc gcagcccgcc gtgtcgcgct gggtagtctg
     6301 ctgtttaagc tggtgggtag cctgattatc ctgccgtttg tccatttgct ggcagagaca
     6361 atggggaagc tgtcattgcc aaaagcggaa ctggtgatct atttccacgt cttctacaac
     6421 cttgtacgtt gcctggtcat gctgccattt gttgacccga tggcacggtt ttgcaaaacg
     6481 attattcgcg atgaaccgga actggatacc cagctacggc ctaaacatct ggatgtcagc
     6541 gcgctggaca cgcccacgct tgctctggcg aacgccgcgc gcgaaaccct gcgcattggc
     6601 gacgccatgg aacagatgat ggaagggctg aataaagtga tgcacggcga gccacggcag
     6661 gagaaagagc tgcgtaagct ggcagatgat atcaacgttc tctacaccgc cattaagctg
     6721 tatctggcgc ggatgccaaa agaagagttg gcggaggaag agtcgcgccg ctgggcggag
     6781 atcatcgaaa tgtcgctcaa ccttgaacag gcctccgata tcgtcgagcg catgggcagc
     6841 gaaattgccg ataaatcgct ggcagcacgg cgggcatttt cgcttgatgg gttgaaggaa
     6901 ctggatgcgc tctatgagca attgctcagt aatttaaagc tggcaatgtc ggtgttcttc
     6961 tctggcgatg tcaccagcgc tcgtcgtttg cgtcgcagca aacatcgttt tcgcattctt
     7021 aatcgccgct attcccacgc ccacgtcgat cgcctgcatc agcaaaacgt gcagagcatt
     7081 gaaaccagtt cgctacattt aggcttactg ggagatatgc agcgcctgaa ctcgctgttt
     7141 tgttcggtgg cttacagtgt gctggaacag ccggatgaag atgaaggacg ggacgagtat
     7201 taatattgtg aatcctgtag gcctgataag acgcgtcaag cgtcgcatca ggcactatga
     7261 acaagatgcc ggatcatgca tccggcaatt cacttaaaaa cggtgaccag cttccagcgg
     7321 aaccgcttct tcaccagcct taaacacata agtggtgttc gggccaccca gcgtagcgtg
     7381 acctttactc actgtgatcc agttaccttc cggcagacca ataatcgtca gttctggcgc
     7441 gacgaccagc agttcgcgaa tacgctgctc acgggtttca cctttatggc cttccggcag
     7501 cgcgttggtg aagtgcgggt tgatttgcaa cgggaacaga tttagcgcat cgaaaccttg
     7561 cggatcgaca atcggcatat cgttggtggt acgaatggtt gggcaagcaa ggttagcgcc
     7621 tgcgctccag ccaatataca gcgcgccacg tttaaccacg tcagtaattg gagccagcag
     7681 cccgcgctcg cggcactgtt tcagcaactg gaaagtatta ccgccgccga caatcacaat
     7741 ttccgcattt tcaatcgcgg caacgggatc gacaacgcta tgaataccgg tgacagaaac
     7801 acccagcgga gcgagaaccg cagccgtttt ctctgtgtaa tcatcccagg tctgcgttac
     7861 gccaaacacc gctgagcggc gaccctgcaa ctgttcagca attagcggca gtgcatgttc
     7921 cagccaggct ttacccggca gcgtcgagtt actcaataaa agcagttcca ttacttctcc
     7981 ggttagtaag aaatcacgga aggcatgcta ccactgcgaa tttaaccact tactgacatg
     8041 gctcacgatg ttacgtattg caccgggcgc tgagactgat gacaaacaca gcattgcctg
     8101 atgcgctacg cttacctggc ctaccagata actgcaatat attgaatttt caaaattatg
     8161 tagaccgaat aaggcttcat tccgcatccg gcataaacaa tgtgtacttt gccaccaatt
     8221 tgagcgcccc ttaaggcgct gatatttttt atccttctcg tgcaaattgt gctgcttcag
     8281 caatcagccg cacatccggt cgcgaagttg ctggggcgac cagccagcga catgcagagt
     8341 taagtagcgc gattaatcat gggaaattgc ctctgcatgg gcgagcaggt attgctccgc
     8401 ctcaccggac cagatcccct gatgacgcgc cagaatcgcc gccagtgctt gatcctgaat
     8461 ctgcgtgagt gacgtcagcg gcaggtattt cccggtatac accagctttt tgccgccgcc
     8521 gacggcaggc aattcaagcg tggtttcgcc cgcggcattc agcccaagaa tatgtgttac
     8581 cacttttgcg gcctgcactt ttttctcttc gatcagtttg accgccgcgc gcatgtcgtc
     8641 ggtattgccg cctgacgtgc ccacgtagtg ggtaaatgca taatgcacat cgtagaaatt
     8701 aattggcgcg ctgaaatgtt tatcctgcgg tccggcgaag aaattcaggc aaccatctgt
     8761 cgccagcaag gaagaggcga gagtcaccag tccttcatta ggcacaaaga cgaaaatatc
     8821 atcgaagccg tgaccgccac tcagcgccat taacgtatca aatgctgcat cggtggcatt
     8881 gagataatga atcagtgttt gcggttctga tggatagtgc ttgcgcgcat aactcaattt
     8941 gtcgttgtcg gtatcggtaa tgacgagcag cgacgggtta actggtccat gcagcgcata
     9001 atcaatcgcc aacagtccca ttggtccggt gccgccgagg atcagcgtgc gcccttgcgg
     9061 gcgaatcccc atcgtgtggt tataactacc ttcctgaaga tgatagttgg cgttgaacgc
     9121 gccaatcacg caggaaagcg gttcaaccag tgagccttca aaataggttt cgccgtcgta
     9181 tgccagcagg caatcttgtt ccatgacctc gttgggaata accacatgcg tcgcttcgcc
     9241 gcctacccac gggaaggagt agccggggca atccgggcgg tcggggagtt gcaggttggc
     9301 ctgaatcaca taacgctgac ccggctggaa tttgtgctgc cactttttac ccacggccag
     9361 aatatcgccg caaaattcgt ggccgatgat gattgggttg gtcgccacat cgtcgggtac
     9421 ttttttatgg ttttcaccca gattggcctc tttccaggaa gagaggcaca ggctgtcagt
     9481 gaccaccgtt gcgaggattt catcctcctg catttcagga aggtcaaagg tttccaggcg
     9541 taaatcacgt ttaccataaa gacgcagagc tgttgttttc ataaaccacc tcatgttgag
     9601 cgatggcctc ggcagtcgtg ccggggcgaa gaatcaggac aggaagccca gagcattgcc
     9661 gataatccct aagccaaaca gggcgaaaat cagccatacc gggttaattt ttttgttgag
     9721 cagacgaacc attagtagcg tcagaccgag cgccagcaaa ccagggcaaa gttggtcgag
     9781 aatgttctgc acggtcatgg tgacggtgga gccatcggcg gcatgcgttt gtgaaaccac
     9841 caacggtacg ttgattgacg tccatttggt caccagcacg cccatcacaa acagtccgag
     9901 aattgacgcg ccttcggtga gtttttgcag cacattcccg cccatatcgc tgacgatatt
     9961 cacccctttg cgaaagccga gctgtaggcc ataccacttc atcgccagac gcaccgcgtt
    10021 gaaaataaag aagaacagca gcgggccgag aatgttgccc gaaagtgcca gagatgcgcc
    10081 gagcgcggcg gtaatcgggc gcagcgttcc ccagaccagt ggatcgccaa ctcctgccaa
    10141 tggtcccatc agaccgactt tgatgccgtt gatggtaccg tcatcaattt ccgcgccgat
    10201 gaccgggcca catacggctg gcgtggtatt gaagaacacc aggtgtcgcc tgagcgccgc
    10261 aacctgatcc tcttttaatg ggtaaagtcg cttgatggcg gggatcatgt cgtagcaaaa
    10321 gcccagcccg tgaatacgtt caaagttaaa ggacgcctgt tgcaggttgg agcgcagaaa
    10381 catgctcacc agatcgctgc gtgtaatttt tctctgttcc atgatggctc cgttaatcgt
    10441 caagttggtc aagggcggtg gaggcggtgg cctggagttg aggttcagct ttacgccact
    10501 gtggatttag ctggatgtag accagggcca tgatcacgcc gacgccaccg aacgccagca
    10561 ggctgagatc gaggtagcca cctgcgagga aaccgaggaa aaagaaaggc atcaaatatt
    10621 tcacgcccat catacgtagc accatggcgt aaccgaccac cacgataaaa ccgccagcaa
    10681 tctgtagtcc tcgggtcaca aattcgggaa tggcgtttaa catattgctg accatatcgg
    10741 cgctgacgaa cagtgagaca atcagtgccg gaatggcgac gcgcagcgcc tgcacgccaa
    10801 gcgcggagac atgcagaata tcgagagtgc gaaaccgcgc ctcttctgct gctttatccg
    10861 ccgcgtgctg gaacactacg gtgatggtac gggcaaacac cgtcagcact tggcctgccg
    10921 cagccacagg caacgcgatg gcgattccgg tggcgatgct ttgctgacca acgataacca
    10981 gaatggcgga gattatgctg gcgagtgcag aatccggaga ttgcgccgcg ccgacgttca
    11041 tccagccgag agctatcaat tccagcgtac cgccgagtat tattccggtt tttaaatcac
    11101 cgagaattaa accaatcacc gtacaggcaa ttaacgggcg atgagtctga aattcatcca
    11161 gcacgctgcc cattccggca atacaggaaa aaagaaatat agcaatgatt tgtagggtac
    11221 ttatttccat gatttgtcac cttttaagtg tcgtcatccc tgcctgttaa agcatatgta
    11281 cacaggcgct attatttaat ttgcgattaa ctgttcgttt attttgtcga taatattgat
    11341 tgagggatct gacgctacga cgcgtaaatc cagtatgaca ccaagattat ttaactcatg
    11401 aaatgcgtta atatcatcat catctaatga aaccgctttg gttaactgtt ttttaccggg
    11461 ccgctaggcc atgccgccaa tattcagggt gcctattttt accccctggc gaaccatcgc
    11521 taaggtatcc tgtgggcgag tgaaaagata aaaaacggtt tcgtcctgat attgcgggtt
    11581 gtgataaacc gcgacggctt tttcaatatt gaccacgtta accttcattc ccggtggcgc
    11641 agcctgacgc aataatgtcc ggcgcacttc atcgttataa acttcgtcat tgcagataat
    11701 aatgcgctgg gcgttagcca ctttcgacca gacggtagtg acctggccgt ggatcagacg
    11761 gtcatcaatg cgggcgagcg taatgttcat cagaaatcct cctctgtcgt tgccagttgt
    11821 ttccagacaa cgcaggtttg ctttgcgatt tgcgtcaggt gttcacacag ttcatcaaca
    11881 ttcatgctgt cctggttatc aaccagttcg agcgccagcg gcagagaaag gccgctgatt
    11941 acccgcaaac gaggattgcg catcgcaagc attgctgccg cattccacgg gctgccgcac
    12001 tgtaaatcca cggcaatcag ccactcatct tgattgtgaa tgcttactaa cttttccagc
    12061 tttaccgcaa tgtctccggc gttttcaccg ggcacaaatt caacggcttc aacattggca
    12121 ttgccgtaaa ccatctgaac cgattccagc atggcgcaag ctaatttgcc gtgggcacaa
    12181 aaaatggcgt tgaccataaa gcctccttag ccgcgcgttt tgccgcccgc aatgttagtg
    12241 gttactccgg tgatgtagct ggcgcgttca gacaacagat aacaaacaaa atcagctact
    12301 tctgctaatc ttccggcgcg cccaatagga atggcgtttt tggtatagcc ttcacgcagc
    12361 tgctcgacgg tgatattgcg cgtccacgcc agcgcctctt catattccgg cgtgcgtagc
    12421 ccggtttttt ccagaatccc gggcgcgata ccgaccacac ggataccgtg tttacccagc
    12481 tctttcgacc aggagcgcgt gaagctattg agcgcggctt tggtagcggc gtaacagctt
    12541 tgtccttctg agccttccag cccactttct gaggaaacat tcacaatcac gccatcatgt
    12601 tgtttgacca tctgtcgcgc caccgcctgc gacatcagaa aaacaccttt ctggttgata
    12661 ttgaccattt tttcgaatgc agcttcgttg agttcatact gcccggcagg cgctttctca
    12721 tcgaccagca gacgcgggaa attgaccccg gcgttattga ccagaccgtc gatgcgacca
    12781 aaacgctgga taatttccgc taccgtatga tttacctctt tggtgctaga aatatcggtc
    12841 ggccaaaact gatagccttt atgaccttca tattgaccat cgccaccgtg aatatcgacc
    12901 atctgtacat tcgcgccttg tgctaataat tcctctacaa tcgccagacc aataccggat
    12961 gcgccgccgg tgacaataat gattttatcc tgcaaattta accacgtttg cataacagac
    13021 tcctgatttt agataatagg gacccgccgt aatttttatt acggtaaaca tcgggaagat
    13081 ttttatttca gtaatagttc agctgtgctg ctattcgtta ccagacaatt aatatatttt
    13141 ccacgcagtg caccaataat tccgctgtat ttttcttcac tcatggcaat gccaatggaa
    13201 tatcgcgcct gctttaattt attcatttcg atagagagc
//
LOCUS       DADLSB010000106        13057 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.106, whole genome shotgun sequence.
ACCESSION   DADLSB010000106 DADLSB010000000
VERSION     DADLSB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.106"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1808)
                     /locus_tag="JN331_002891"
     CDS             complement(<1..1808)
                     /locus_tag="JN331_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7260991.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGTLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGN"
     gene            complement(1856..2065)
                     /locus_tag="JN331_002892"
     CDS             complement(1856..2065)
                     /locus_tag="JN331_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HAY7260992.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(2161..2535)
                     /locus_tag="JN331_002893"
     CDS             complement(2161..2535)
                     /locus_tag="JN331_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7260993.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(2541..3257)
                     /locus_tag="JN331_002894"
     CDS             complement(2541..3257)
                     /locus_tag="JN331_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7260994.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            complement(3324..3668)
                     /locus_tag="JN331_002895"
     CDS             complement(3324..3668)
                     /locus_tag="JN331_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HAY7260995.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(3665..4111)
                     /locus_tag="JN331_002896"
     CDS             complement(3665..4111)
                     /locus_tag="JN331_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAY7260996.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4108..4458)
                     /locus_tag="JN331_002897"
     CDS             complement(4108..4458)
                     /locus_tag="JN331_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7260997.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(4468..4794)
                     /locus_tag="JN331_002898"
     CDS             complement(4468..4794)
                     /locus_tag="JN331_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7260998.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(4791..7044)
                     /locus_tag="JN331_002899"
                     /pseudo
     CDS             complement(4791..7044)
                     /locus_tag="JN331_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6990..7211)
                     /locus_tag="JN331_002900"
     CDS             complement(6990..7211)
                     /locus_tag="JN331_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7260999.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLRKAEYITARRPA
                     GQKKKHDAEHSECGTFCDEPEKTRNQDVM"
     gene            complement(7256..9193)
                     /locus_tag="JN331_002901"
     CDS             complement(7256..9193)
                     /locus_tag="JN331_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7261000.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVKGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRQFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEAMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTEFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(9257..10918)
                     /locus_tag="JN331_002902"
     CDS             complement(9257..10918)
                     /locus_tag="JN331_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7261001.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYGAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(10915..11478)
                     /locus_tag="JN331_002903"
     CDS             complement(10915..11478)
                     /locus_tag="JN331_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAY7261002.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(11767..12132)
                     /locus_tag="JN331_002904"
     CDS             complement(11767..12132)
                     /locus_tag="JN331_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7261003.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKHHIWLR
                     DAGQCRLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     TTLPEVSGGSGRADDIIGL"
     gene            12174..12374
                     /locus_tag="JN331_002905"
     CDS             12174..12374
                     /locus_tag="JN331_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HAY7261004.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            complement(12506..12832)
                     /locus_tag="JN331_002906"
     CDS             complement(12506..12832)
                     /locus_tag="JN331_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HAY7261005.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
BASE COUNT         2807 a         4212 c         3145 g         2893 t
ORIGIN      
        1 tcattaccca gccccagcag acggttgaat tccgtatgct gaccgttcat aaccagcatg
       61 gactggtaca ccttattctg ctctgccgcc tgctgacgaa ttaacgccac acgacggtct
      121 tccagcccgg caagcacatc ctgaatggac tgcgcttttt cctgcatctg tgccagacgg
      181 gactgctcaa cggcaagctg ctctgttgcc tgagcaagcc cttccgttac ggtcttcacc
      241 gaggtcagat ggtttatcat gaatccgtca ccggtcgtcc agcccgggtt cgccagaaca
      301 tactgatatc ctgcgatttt ttcctgcagg gatttcaccc gactggcctg ttcatcaatc
      361 agccggttct gctctgtcag cgccgcccgt gttcgtcctt cattatctga ggcttcaggc
      421 aaagacattg acggcgtttt atgcgcgatt tcatctatcg tcagtgcata ctggcgcgca
      481 gactccctgg cctgctcctg attctggtac agcgtgtacc atgctgcagc ccccagcatc
      541 accagtccgg gtacgccacc aaccagcccc agcgcaccgc tcatcagacg tgagcccacc
      601 gccgttgtac tgttcagcgc attctgggcg gcgcttctgg cagcaatatt tctgttcagg
      661 cgttcctgtg tggccgccag acgggcctct gcagcaatct gcatctccgt cccgcgggct
      721 gccgccacgg cctgctgagc acggtacacg gctgcccttg cccgcgccgt ggcaatctgc
      781 gttcccctga actgtgcttc cgccagtgca acttcattac gtgcagccgt cacaagtcct
      841 gccgtggcag acatcgctcc ggaggccata ttgccaaagt accgggccac cccgacggca
      901 accagcgcac ccacagctgt tgccacatta tcaatctgtc cggcaacacc gttcagcatg
      961 ccggagagcg tttttgtcac cccgctggcc tcattcgcac cgcccaccca agccataaag
     1021 gcgttttcca cctttgtgat cccgtcagag accgtttctg gcatggcggc atattcatca
     1081 cgcaatgtcc ccagctggct gattaacgcg ggcacgactt tatccgccgt cagttgacca
     1141 tcgtccgcca tcgccttcag atctttacgg gccacgccca tgcctgcagc cagtgcacga
     1201 atgatccggt caccactttc attgaccgaa ttaaattcct caccgcgcaa cacaccctgt
     1261 gccagcgcct ggctgaactg ggtgatcacc gaacccgcct cagccgtact ggcaccggag
     1321 attttcagcc ccgtggaaat ggcctccgtc accttcagta catcaccggc actgtaacca
     1381 tattcacgca tcgaggcagc cgaacgggca aacagggccg cattatccga aaaagcggta
     1441 cctgtccgct gactgatatc catcagcact ttctgtgatg acgaaaattc atcggatgac
     1501 tgtgatgcct gtttcagacg ggcattcacg gaactccact catcagccag tgaaatcagg
     1561 tgtccggtgg caaaggcacc ggcaaatgcg ccagccattc cgacagccga agcgcggatt
     1621 tccgtcaact ggctgttcag ttctgccagg gcacgtcgct gctcccgggc tgccgcagcg
     1681 gcctgacgcc cgccattctg cagggtccgg taatattcac tgcccatacg ggaagcccgc
     1741 tggatctccg actggaatga ctgtgaattt gccgaaattt tgataatcag ttcacgtaac
     1801 gtcgccattc acctttctcc gggcgtaaaa aaaccgcctc agcggttctc atcattcatg
     1861 actgtgctgc aaagctcagc gcgtcttcca gcgccgcaaa cggatccacc tccggcttat
     1921 cctcatcctc gccccagcag agcatggcgt ccttcagtgc aacattcatc ccctgtgccc
     1981 cgaaaaccgc tttcacgatc tgtgcattac ggatatcccc gcgctcatca cccagcgggg
     2041 acaccctgtc aaactccatc cacatcatcg ctttgctcgc actcaggctg tgccgcagtt
     2101 cggataaggt gcgccccaga cggagcgcaa gtcgcatcag aaagcgaatt tccgggcggg
     2161 ctactttttt ctggccgact ctgcatcagc gatcagttcc agtgcctgac gcagcaaccg
     2221 ggcatgtacc ggaccataga cggccagcac ctgctcacgg tcgtccggag tgaacacccg
     2281 ttgcaggtca gtatcacaca ggacatcgca gaacagcgtc acatccgctt ccaggttacg
     2341 gcgggttttc gccaccaccg acagggtatc gtcatcctct ccatcaccat tgagcacttc
     2401 ctgccacaga taccaggcct ctgccgaagg ctcccgcagc accacgctga catttcccca
     2461 ttccggcacc ttcaccgttt tatgacggaa ccctgacagt ctggccagcg ccagtgtttt
     2521 cagatctttt gccataagcc ttatccgccc gcaccattaa ccgttactgt acacgcatca
     2581 gaggtaatgc tctgcggctg ttctgcagaa tccgttacct cgcaggtata agccccctta
     2641 tcacctgact gcgtattggc tttactgaaa gtgtcagtag tctgtccctc taccggctga
     2701 ccatccttct tccaggcgtg tttataaggc ggcgttcccc cgttgacact gactgacatt
     2761 gtcagcagcg caccggtatt cacggtaagt gtcttctcca gatttttcac aaacgccagc
     2821 ggtaccacat aggacaccgg tttacccttc aggcgaagtg aaaacgttgc agccaccacg
     2881 ccgttggtac cggatgacca ggtgtgctga cgcacttccg ccaggaactt aaagccctta
     2941 ccggacggaa actgcacctt aaacgcatac accgtgtcat tgtcataggc atcacgcagg
     3001 gcgttctggg cctgattcag ataaaaatta cccgacatgg aaatctcgga cgacgccccc
     3061 agaccgttga tgttctcctg ctctgtggag cagagcgtgg tcacatcaat atcctgtttc
     3121 tgaccggcgg tgaactggac ttccttgatg gtgcagtcca ggcgcagata ttccgcctta
     3181 tccatagttt cagcagtcgc cggggcagat gaaatcatca cctgcgtcag ctgtgagcgt
     3241 tcatacaaag cagacattct gcctcctgat aataaaaaac ccgcacgcgg cggggtatgg
     3301 gttttgtaga aaaaaagaaa aagtcacacc gtgacctgaa actccaaggt tgcacggtaa
     3361 cagcggtttt ccggaatata gtcctgcatt tcactgacgg atcccggggc cagcagcatt
     3421 atggcttcac gggcgtcctg acgtatctga cgcgcctgcg tcacagtccc ggcataaacg
     3481 tctatctgca ccgacactga ggactccgcc tgcccgccca tcacgtccgc agacaccgat
     3541 gaaatcaggc tgaaaaccac ccacggaagc gccaccgacg gcctgccatc cagcaggggg
     3601 accacatacg ggtacacctg cccgccggca agatgcgcca gatgaggata caaatccgcc
     3661 tccgtcatcg tctcagtacc tcatcaatgg cccggttcat ccgagcaatc gccacctgcg
     3721 ctgcctgttc actgcgcaca tcaaatgccg gacgcacaaa cgggtgcggt ggcatattca
     3781 cggtccccat ttccacaaac cgccagtaga aagcattgcg cgggttatcc gccttcatgg
     3841 tgttatcgct gttaccggtg tccggattaa caccacggat atggacaccg gattccatcc
     3901 cgccatcgcg ggagcgccgg gaaaggacca ccacattgcg gcgcagtttc cccctgcgta
     3961 ccggtgcccg tgacaccact tcttctttca gcacattcgc ccccgcacgg gttgcctcac
     4021 gcagcacccg gttattttcc gcaccactca gaagctgcaa atcgcggctg atgtcctcca
     4081 gccccgaaaa atccagcagg gtttcgatca tttttcccct cccagccgac agagaatttc
     4141 cagacgccca ccggtcgcat ccggcacggg cagcccgaca acgttcagga tccggtcacg
     4201 ccatggacca ctcagcacat gaagtcgtga cgctgccgtg atttcccggc cggactgacc
     4261 gcgcacccag atgcggattt ccgcctgcgc catttccgca ccggactgca tccgctcccg
     4321 gctgctcctg ccacggatat ccgcatgaat tttcccgcat gacacccatt cttccgtcat
     4381 ttctccggca gcattacggg ttaacaccgg gttcagaaca cttatcatct gtgtcagacg
     4441 acctgcagat attgccattc cccctcctca taacaccgtc ggacaacgca aatcgtaaat
     4501 cagcacggaa acagaaaacg gcagctcccc ctgaatcagt tcttcccgct ccgcaagatc
     4561 cggattccgg tacagcatcc cggtcagtcg catggcagcc cccttcatcc gggttaatgc
     4621 ctcgcccggg atcagttcac cgtcctcacg aatcacttta tcccggctgc cctgaatgta
     4681 ggccagcagc acggcggtag cctgacgaac cttgtccatc agcatgtcat catccgcgtc
     4741 atggtcgaca cgcagatgtg ccttgatctc ttccagtgtc agtaatgccg tcattttccg
     4801 cctcctgcat cccgtccccg tttggcagcc agcgtccagc cagatgcatg cgcatccccc
     4861 ggtttatcgg cggtcatact gttgcagtgc cacagcgagc ccccccacgt caccgtatca
     4921 ccggggtggt aggtttcacc ggctctgaac acaccgcggt agagcatcac cagcagggaa
     4981 aatgtttttt ccgtacgctg gccactgctc tgccggacca ccacagagaa caaccgttca
     5041 cccgtcatgc tgacgtcaat atccgccacc ccgtcaacca ggcattccca tccccgcatc
     5101 ccgtgcgttt tttcatacgc ccgccagagt ccgccctggt gtgtggcata cgtgccccgg
     5161 ggaaaggatt tttgatcgtc aatggcgggg agtatttcca gtgccgtggc atcacgcccg
     5221 tcctgcggag ccggcagggc actcaccgca tccagaaccg ccttctgcag aacatccgga
     5281 tcgtagtcac gaccatcacg cggaacagga atatggctta ccgcctcctt caccatctgt
     5341 tcaagcatcg gacgcacatc atccggggtg agacttttac cgtccgccgg ctgtggaata
     5401 tttgcgaccg catcattcac cgccttctgc agtacttccg gatcgtagtc acgaccgtca
     5461 cgcggaacag ggatatggct taccgcctcc ttcaccatct gctcaagcat cggacgcata
     5521 tcatcgcccg tcacgcactt ctgtaatacc acagacaggg acgccagttt ttcttcaaac
     5581 gtttgtgcct gcgcggctat tttcccctca aatgtgcgct gtaaatccgc cagcactgcg
     5641 gaaaattctt cacccagcgc acggataatg gacagttcac gttcattcat tttttcagaa
     5701 tccccctgaa catcgccttc accgcatcat gctctgtttc agtgattgcc ttattaccgt
     5761 cagatgcgct gtcaggcggt tgcgctaagg ccgttttccc ggtcgacgcg aacggatctt
     5821 cacgggcatc acgacgggac agcgcctcca gactgtagtt ctgctgctga agatacagtg
     5881 catcacctcc cgcaaggggc ggcaggttct cacgtttacg ggcctcattg ggcgtgagaa
     5941 gcgtattttt caccgactca cccagcgttt tcatgcgccg ttcgctgtcc attctcagca
     6001 gcgtggtgac gtcaaactcc gtactctcgt tttcccccgt ttccagcgcc tcatccagta
     6061 acagttcaat ggactcaatc agcgtctgca ggcactggga ataatactgc tgctccagcg
     6121 cctccacgtt gtcactggaa ggtggctggc caacgccaat cttgtaggcc gggacacgga
     6181 acaccgaaca gacaatttca gcggtcatct tcagttgttc caccgtctgc gcatccaccg
     6241 gtgaaaacgt cgtggggttg tattttgccc cgttgctcaa aatggccgtt ttccccgcat
     6301 tttcgccggt atacccgctg tcccagttgc tcttcagttt tttcgcattt tcttccgtaa
     6361 tactgccggg gatctcaatc accccggacg gcctgccgcc atttctgaaa aaagacgtcg
     6421 aattttcctg aatatgatgc ccctgcgtgg ccgccagccc ggcggcatac accggcggca
     6481 gccccacaag cggatgaaaa aaacagttaa accggtcgct tcccgggcag gcaccgtcac
     6541 cgcctccgtg atcccgcagt tccggtccgg cgtgatgcga tagaacacct cgccgtcatc
     6601 cgccaccaga ggttcaaccc ggctccagtc cagaatacgc agttctttga tctgcccccg
     6661 ggagttacgg attttcagca ccaccgtatt gccgtgacgc agtttggcgt tcagccacag
     6721 ttcaaaaaac tggatacgat tctgctgtgc attgggacga cgacagagac gggcaatatc
     6781 cccctgccgt ttttcacggc ggatcccctg tgtatccgtc tgcatcaggc gcagccgcat
     6841 tttggcgata tcctgggata tcagcgaaat gcaagaaaac accgcatgaa aggagaggac
     6901 actttccgga tcggctttca caccctgctg ccaggcgcca gcaaagggct cagccaccgc
     6961 ctgaaacagg ctggtccagc ccacctcttt tacatcacgt cctgatttct ggttttttcg
     7021 ggttcgtcgc aaaaagttcc acattcgcta tgctccgcat catgtttctt tttctgaccc
     7081 gccggacgtc gcgctgtgat gtactccgcc ttcctcaggc gaaccagcac ctccgcacac
     7141 ggctgtgcca catcacggat atccccggcc cgggcatcat gcgtgccctg cagatatcgg
     7201 atctttgcca taacctgtta cgggaggcgc acgcctcccg tcctccttat cagactcagc
     7261 cgccggacgc actgccgtag ttcactccgg tgatcaccgc caccgccgcg gtacggcgac
     7321 gacgccagtt gatccagcgc tccgcacgga tggccacact gcctgtctgg aacatggaaa
     7381 ccagctccac cggcgacggc gtggtactgt cgccggtcgg ctcagactgc atttccagtg
     7441 atgcctcgcg ggacatatcc actgccacgc cgccgtcatc cgccagataa atatccgggg
     7501 cattcaccag caccagctgg tcacccacgt actgggagac aatcaccggc agcccctgga
     7561 aggagccacc cagcagggtc atgtccgggt attctttctg acccagcgca tttttacgca
     7621 tggacagcgc cagggcattg gtgctggaca tcagccagac agcaccggtg ggctgcaggt
     7681 ttgctgccac aaactgtcca aacgccgcct ctgcatccgc atccgggtta ccgcttgatg
     7741 ccgtgccctt cacatcatgg gtgatggacg ccggggagac atctgccacc gccgcttttt
     7801 tcgggtccac aaattctgta tccagacgcg ccaccaccgc ttccgccagc gcattacgga
     7861 ccagtgcatc agcagccgga ctggaaaaac ggatcaattc ttccgtcagt accgcaatgg
     7921 ccgacacctt cgcatgactg aaggtgatgg attcaaaatc aaacttcgtc aggggttttg
     7981 ccttaccctc acccacccag ccggcagcac cgcccgacac ctgggcgtgc acacggatat
     8041 tgaacggcac ctgacgaagt gcagggatcc cgccctgacc aaatcgcccg ataatggtct
     8101 gaggacgcag gtaatcaata aagtcctgtg cgtattcctg atattcagac aggctgcctg
     8161 cccactgcgg atccgtggtg gtccccgcgc ccactgccga tttcaggaca tgatgcagac
     8221 gactgtcatc cggatactga cgacgggcca cttccagggc ttcagagcgg acacctttag
     8281 ccgcagccag cgatttggca aaacgtgcga aaccaatccc cttatccagt ttctgctcca
     8341 cacggatcac cggcgcagaa gccaccgcgg ccacattccc gttaccggcc tgtttcaccg
     8401 gctgcgccgt ggcggcctta ccggcttcca gttcacgcag gcgcttcagg tgcgcatcca
     8461 cctgacgtat ttccgctgcg gtgttgtcgt aatgctcttc ctcctccaca tccagcgtgc
     8521 gcccttcctc tgcggctttg gtcatggcct cctcaaggga ggctgccagt gctgcacgct
     8581 tgttttcaaa acttttaatc tgttcgccaa tattcattat ggtcttttcc ttatgaaaaa
     8641 cggttgttga ctgtgccgca gcgccggcag aagatgcgat tttcaccacc ggtttccggt
     8701 tgccggacgc ggcagaaaac tggcggtcgt aagatttaat ggtccggatg gtgcattccg
     8761 cattcgcggg cacggtgacg gcagacacct ccatcagctc ccagcgcaga aaatgcagtc
     8821 cgcctccgtc cagaaaggtg tattcatgag gacggaagcc cacggacagc cccttgacca
     8881 gcccggtctt aatggccgcc caggcctcat ccagccgggc agccagttgc gatggcatat
     8941 ccggcacggg cttcgccagt gttgccgtga tttccagccc ttcgctgacc cggcgcaccg
     9001 tacactgccc caccgggcgg gaatggtcat gctgccagag aaacgggatc gcactgccaa
     9061 actccgcgcc ctccggctcc aggatgtcac catcccgatc cggagaaggc gttgacgcaa
     9121 tcccggtgat cacccgctca tcctcactga aggatttcac cgtcagcagg gagcaggccc
     9181 gtttaagagt cacatcagcc tcctgaaaat aaaaaaaccg ccgcagcggt ccatgatggt
     9241 tacagggtga acagggttat atgaaaaaaa ccgcatattc tttctttttc ggctccggat
     9301 tcagggacat cagggatacc gcattgaaga gcgccatcag cgggtcaatt ttcccccgcc
     9361 cgctggcctg tttggtaata aggatagcgt tacctttagg ctccacccgg gcattgccaa
     9421 cgcaccaggc catcagtggc tgaccaccat gcaccagtac tccctcagcc agtttgcgct
     9481 cggtggtttt tatggccccg cccagcttcc agccctggct tatccccaca acaattccgt
     9541 cggggatccc ggcttccgcc agtgaatcca gaatctgccc cacacctgac gggtcaatac
     9601 cgatatgctc cagtaactca gcctcatgaa tacgacgcac atactccgcc acttccgccg
     9661 tgtcatcccc gacccgacgg acaatcgtca tgtctccaca ggccacaaaa tcctgaaaac
     9721 gggatgcctc actcttccgt ctgaccaccg cggtttcatg cgcccaggca tggccccagc
     9781 ccagccattc gcgggtttcc ctgtcacggc caatcacgta cattcccagc agatcatcca
     9841 ggcccccgcc gtcaatcccc accgtcacca catcagcgcg ctgcaggata tcgtccaggc
     9901 tgacgcgcct gccctgctgc tcccagaaat ccgcacccgc ccagcggtca gaacgcaggg
     9961 caagaccaat ttccacattg gcatgttttg acatgaagcc acgaaatgct tcctcaccag
    10021 cctcccgggc tttacggtac tcccggtaca gaaaagcctc atccaccgaa taaccgagat
    10081 tcggattgac catggcgagg ttttccatca gcaggtgagc cccgctttcc accatttcag
    10141 gagggtgttc aaatatcacc ggcagaaagt gcggatcatg aattttgccg tcacggacat
    10201 cccgggcgta ctgcagtttc tgtctgaaca ccccggctgg cggctcattc gactgggtgg
    10261 tcgtatacac cacaaaccct tccgggcggg aggcaaggcc gcctatggct tcacgtaaca
    10321 tgtcctccgc tttgtactgc ttgccaaata accacagttc atcaatcagc gtccccacgg
    10381 acttgatacc ggataccgta ttcggatcgg cagccaccac cttaagggtg gtgtccgtca
    10441 ccctgtgggt gatggtccgg atatgtgtct gcacctgaca gaggtcatcc agatcatcgt
    10501 cccgtcgtac catatcccta gcagggttga aggcgttagc cgccacctcc acggtcgggg
    10561 ccagaatggt atagcccgcc gcctgccgcc agttcagtaa cagcgccgtc atcatgatcc
    10621 cggcagccag cgtggacttg ctgtttttct tggggataag gataaaaact tccttgatat
    10681 ggcgtacacc ggtctgcgca ccgtaggagc caaacagggc cgccaccagg tcaaacaccc
    10741 actgtgcgca ggactccccg aacgtcgggc tgcccggtgc atccacaatc cgcagttgtt
    10801 taaaaatcgc cagggcatgt gcggcctgct ccggataaat cggagccgga ataatcgaca
    10861 gccccttttt caggcgctct gcccagtccg ggcaggccgt gctccacaca ggtatcatcc
    10921 gttgccctca ttatcattat tcaccaccag gcggggtggt ggtggcaccg caaaacggtt
    10981 agccgctttt ttcgccgcgt caccttttgc cgatttttta ccggcatccc cttttttgtg
    11041 gtgcgtgaac tgcgccagct tataagccgc atccagcgcc agcctggggt cggtattaat
    11101 gttctccacc agaagacgcc ccatcgcttt caccggatcg ggaagaccgt cctccatata
    11161 ctcaatacca ggagatatca ccacgggcgg tggcatctcc ggatttgttt cgtccggctg
    11221 tggtattgca gccgcctcac ggcgacgggg tttatcctcc ggctctgatt ttttctgccg
    11281 ataaacagga acctcatcca cctccaccgt ctcgcactgt ttacgggcta taaacgcaag
    11341 cacctccgga tcttttgcca gctgcgagcc tttaaccctg gctgtcttcg ccgaataacc
    11401 ggcggcaagg gctgacgctg ttttgttttt cccggacatg agcgccagcg caaattttcg
    11461 tttttgcgtt gtcagcacag cctcctcccg ggtccagaac gcactcagcc gggtatggtt
    11521 cagcccattt ttcccggcgt ctcatgccgc aaatgttaac tgctgcctgg ttaacatttg
    11581 ctgaaaaatc ctgttaacat tttttccacg caacaaactg aataataaag ataaaaaccg
    11641 caaaaatgcc cggacagcca gttaacatgt taactgccct gaaacgggaa ttttttctct
    11701 gcgtgagagg gggcgcggtg tccggagcga tcgtttttta cgccgaatga tacccccccg
    11761 gtcgggttac agtccgatga tgtcgtccgc tctgccacta cctccggaca cctccggcag
    11821 cgtcgtgtcc ggcataccac ccgccgcttc acgagcagac ttttgtcgat ggcattcggt
    11881 acagagcgtc cagagattcg tctcctcatt accaccaccg aactgaagtg caattcggtg
    11941 atcgagttca ctgtcacaga ggtcaaccac acgaccacag agacggcact gcccggcgtc
    12001 cctgagccag atatgatgct tgagggaaac acgtgcactg ccactgacac gacgctgttc
    12061 acccttcaga atattcaccc gtcgggtatt cagtgttttg attctgctct ggagtgtacg
    12121 aagctcagcc atgtaaaatc cccgtcatat ggcaatcagt aaaggaaata aatatgtcat
    12181 cgaaaaaccg tacccgcaga accacaaccc gcaatatccg tttccccaat cacatgattg
    12241 aacagatcaa catcgccctt gagcataaag ggtccggtaa cttttcagcg tgggttattg
    12301 aagcctgcag gagaaggctg gcaacagatg caacgcatct gcgcccggcc agcatgacaa
    12361 ataacgagaa atgaacgttc ggttacagga gcaggtaccc actgtcctcc aacaatattt
    12421 catcttcata tccgacggaa caagacttac cctgccggga tgtacagaat aacaacagag
    12481 tgataattaa tttctgatga aataatcagg gtgcagaagg actaaagata aacgttttct
    12541 tcacgccttt acacggcctg tccttctcaa atcgccattt tgccatcgcc tttacaacct
    12601 gctcatcaaa cagatggtgc ggctctgaac gaataaactc aattcgggtg acagtaccat
    12661 cagcaccaat atcaaacctc acatcaaccc gtccctttat ataatttgcc gctgcatagg
    12721 ccggatattg tggtaatgcc ttaaccaact gtcggggcat atctgtttta tgttgcgtac
    12781 agcccataac cagagaagac aacaaaataa ttaacggaag atttcttttc attttcattc
    12841 cccgcacaga taagaataag tcttattcta acaatgccgc cctgtcggtc atcaatcctc
    12901 tgcttaatgg caacgacaat tatccgactt aaatcacaaa tcagacacat gacataacag
    12961 ggcttgcgag gtaacacatc gtcctgtttc ttccaccatc gcaccggact ggcgactatg
    13021 aggggacaac gccgcgctcc gttaacgcgg taaaccc
//
LOCUS       DADLSB010000107        12947 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.107, whole genome shotgun sequence.
ACCESSION   DADLSB010000107 DADLSB010000000
VERSION     DADLSB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.107"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(567..1658)
                     /gene="ychF"
                     /locus_tag="JN331_002907"
     CDS             complement(567..1658)
                     /gene="ychF"
                     /locus_tag="JN331_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY7261006.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1775..2359)
                     /gene="pth"
                     /locus_tag="JN331_002908"
     CDS             complement(1775..2359)
                     /gene="pth"
                     /locus_tag="JN331_002908"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7261007.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2637..2915
                     /gene="ychH"
                     /locus_tag="JN331_002909"
     CDS             2637..2915
                     /gene="ychH"
                     /locus_tag="JN331_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY7261008.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2970..4649)
                     /gene="dauA"
                     /locus_tag="JN331_002910"
     CDS             complement(2970..4649)
                     /gene="dauA"
                     /locus_tag="JN331_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY7261009.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSTLVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4774..5721)
                     /locus_tag="JN331_002911"
     CDS             complement(4774..5721)
                     /locus_tag="JN331_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY7261010.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5872..6723)
                     /gene="ispE"
                     /locus_tag="JN331_002912"
     CDS             complement(5872..6723)
                     /gene="ispE"
                     /locus_tag="JN331_002912"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY7261011.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     SLHRAML"
     gene            complement(6723..7346)
                     /gene="lolB"
                     /locus_tag="JN331_002913"
     CDS             complement(6723..7346)
                     /gene="lolB"
                     /locus_tag="JN331_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY7261012.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7560..8816
                     /gene="hemA"
                     /locus_tag="JN331_002914"
     CDS             7560..8816
                     /gene="hemA"
                     /locus_tag="JN331_002914"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY7261013.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8858..9940
                     /gene="prfA"
                     /locus_tag="JN331_002915"
     CDS             8858..9940
                     /gene="prfA"
                     /locus_tag="JN331_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY7261014.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9940..10773
                     /gene="prmC"
                     /locus_tag="JN331_002916"
     CDS             9940..10773
                     /gene="prmC"
                     /locus_tag="JN331_002916"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7261015.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10770..11162
                     /locus_tag="JN331_002917"
     CDS             10770..11162
                     /locus_tag="JN331_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY7261016.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRHMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11166..11976
                     /locus_tag="JN331_002918"
                     /pseudo
     CDS             11166..11976
                     /locus_tag="JN331_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12012..12866
                     /gene="kdsA"
                     /locus_tag="JN331_002919"
     CDS             12012..12866
                     /gene="kdsA"
                     /locus_tag="JN331_002919"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY7261017.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3251 a         3237 c         3346 g         3113 t
ORIGIN      
        1 acagtggtta aaaaatttgg tgaagatttt ttgtaataat cctaattagt tatgggattt
       61 tcgcttgatg tatctgcata acaaaaacaa tatgcgccat ttttgcatta ctaataagaa
      121 aaagcaaaaa acatcgatat tcatttttgg tcaatggatt ttctggccta tcgttattat
      181 tgtgctactg gttatcattt tcatcttgcc aggatcgcac gtaatcacgt tattcgggga
      241 ctttatctgc gggatattac ccttcaggta agtccttttc tcgccagcat attggagcga
      301 gatcaatatt gatcagctgt gaagagggct gatgtgtagt ggaacaaaaa ctgacgttat
      361 atcactgcta atgagatatc agtcatctga acacttatgt ctttaattat tctcgtggtt
      421 cactgtaggc aataagcaca aaagtgtagg atgttacaag aatgattagg actcggtgaa
      481 atgaaaaatc cacgcaattg cgtggatttt atatactttt gcgctcttca tgagatttag
      541 cgaaacctca tgagacaata aattaattag acgttgaaaa ggaagttcat cacatcgcca
      601 tctttcacga tgtaatcttt accttctgca cgcattttgc ctgcttcttt cgcgccttgt
      661 tcacctttgt aagtgatgaa atcttcaaac gagatggttt gtgcacggat aaagcctttt
      721 tcaaaatcag tatggatttt gcccgctgcc tgcggcgcgg ttgctccaac cggaatggtc
      781 catgcacgca cttctttcac cccagcggtg aagtaagttt gcaggttcag cagcttataa
      841 ccggcacgga tcacacggtt caggcccggt tcttccagcc caagctcctg cataaactcg
      901 tcacgttctt cgtcgtccag ttcggcaatg tctgcttcaa cagcagcaca aaccggaacc
      961 acaacagaac cttctttcgc cgcgatttca cgcacctggt caagatatgg gttgttttca
     1021 aaaccgtctt cgttgacgtt ggcgatgtac attgttggtt tcagcgtcag gaagctcagg
     1081 taacgaatag ccgctttctc ttcagcgctt aaatccagcg cgcgcagcat acctgcgttt
     1141 tccagctggg gcaggcattt ttccaggacc gccagctcag ctttcgcgtc tttatcgcca
     1201 cctttggctt tcttctgtac gcgatgaatc gcacgttcgc aggtgtcgag gtctgccagc
     1261 gccagttcgg tgttgataac ttcaatatcg tcagccgggt taactttgcc ggaaacgtga
     1321 atgatgttgt cattttcaaa gcagcgaaca acgtgaccga tcgcttcggt ttcacggatg
     1381 ttggtcagga actggttacc cagaccttcg cctttcgatg cgccttttac cagaccggcg
     1441 atatcgacaa attccatggt cgtgggaagc gtacgttgcg gttttacgat ttcagccagt
     1501 tgatccaggc gagggtcagg cattggtacg acgcctgtgt tcggctcaat ggtgcagaat
     1561 ggaaagttgg ccgcttcaat accggctttg gtcagcgcgt tgaacagggt agatttcccg
     1621 acgttgggca aaccgacgat accgcatttg aatcccatga tttaaatcac cttaatatct
     1681 taataatcaa cctgttattg ctaacagatt gcagaaatgg aaataacttt gcctattata
     1741 cacggcactc ggcaaaaatg ccgcagacaa cgacttattg cgctttaaag gcgtgcaatc
     1801 ggttcgttgc tttggtcaag ccatctgtaa accacatttc agtacaacgc gccgcttcgt
     1861 cgatggcttc atcaattaac ttctgttcac taacaggcgg tttgcctaac acaaaaccga
     1921 caactttatt tttatcgccc ggatgaccga ttccgatgcg taaacggtga aagttagggt
     1981 tattacccaa tttactgatg atgtctttca gtccattgtg accaccatgg ccaccgccca
     2041 acttaaattt ggcgacgcca ggaggcagat ccagttcgtc gtgggccacc agaatttcgt
     2101 ccggattaat gcggaaaaaa ctggccatcg ccgcaacggc tttgccgctg agattcataa
     2161 atgtagtcgg gactaacagg cggacatctt cgcctccaag ggtgactcgc gaagtataac
     2221 caaagaattt agcctcttcg cgcagcggag cgcgcaaacg ctctgccagt aagtcaacga
     2281 accaggcacc agcattatgt cgcgttgcgg cgtattcagc accggggttc gccaggccga
     2341 caatcaattt aatcgtcacg tttttttgtc ctgagtgtgt acataactgg cgcgtagttt
     2401 actggttgcg gccccgcttg acaaaaaact gcgtatcaaa tgcagataac gtaataattg
     2461 cctgagtgga ctattagaaa gtcaaggtgt tcaggcgttt atttgtaaag ttttgttgaa
     2521 ataagggttg taattgtgat tacgcccgca cataacccac agggtgttgt ctatacttta
     2581 cacataagga agaggggtat tccctgttac aacccagaaa gttccggagg tgacatatga
     2641 aacgcaaaaa cgcttcgata ctcggtaacg tgctcatggg gttgggtctg gtggtaatgg
     2701 tggtcggcgt ggggtattca atcctcaacc agttaccaca gtttaatatg ccccagtatt
     2761 tcgcacatgg tgcagtgcta agtattttcg tcggtgccat tctctggctg gcgggtgccc
     2821 gtgttggcgg gcatgaacag gtgtgcgacc gttactggtg ggttcgtcac tatgacaaac
     2881 gttgccgccg tagcgataat cgccgtcata gctaacaaat aatgcagttt gctgaccagt
     2941 cagtttgcgc tgactggtca atctcgtact tataaatccg ccatcgccgc gcgacgattc
     3001 gggaagaacg cgaggcgtcc cgggatcggt tgaatgcctg cgcgagccat agttcgcagt
     3061 ggctggaatt ccacgttgca cacgcgcagt tcacatcctt cgggcagacg cttcacaaaa
     3121 cgctggaacg catcaagacc accagcatca agtaccggaa cggcatccca cttcagaatc
     3181 acaatccgtt tgccttcaag acgtgactct aggtccgtga ataagccttc agcagcagca
     3241 aaaaacagcg ggccaataac gcgcagaacc aggacatcgt ctggaacatc tacgactacc
     3301 ggtgccaggc gagtcatacg tgcgatacga cgcataaaca gcagcgatgc cagcacgatc
     3361 cccacgctga tggcaataac catatcaaac agcacggtca gtgacataca cagcagcatg
     3421 acgatgatgt catctttcgg cgcatgacgc agcaagtcga ccactttgtg cgcttcactc
     3481 atgttccacg ccaccatcaa cagcagggct gccatggcgg aaagcggcag ccaggagagc
     3541 agtggtgcca gtaccagcag ggcgagaata accagagtag agtggatcac cgccgagata
     3601 ggggaagttg ccccggcacg gacgttagcg gcagaacgcg cgatggcagc agtagcggta
     3661 ataccaccaa agaatggagc gatgatattc cccagcccct gaccgaccag ttcactattc
     3721 gccttgtgct tagtcccggt cataccatcc agtaccacag cgcagagcag agattcgatc
     3781 gccccgagca ttgccattga gaatgccgca ggcagcaggg tgcgaataga atcccaggtt
     3841 agcgtgaatt ctgaattagg cagatcccac ggcagcacca gttgcggcag cagttgcgga
     3901 ataccgttac cctgagaacc atcggccagg acgtagtgga attgcgaacc gatggtagca
     3961 acatgtccgc cgagcaggtt aacaatcccc atcaccgcgc aaccagccag caaggccgga
     4021 aggtgaccgg gtaaacgaat gcccagacgc ggccagaaaa ccagaatgcc aagcgtcaca
     4081 atgccaatgg cagcatcacc cacattaatg gtcggcagcg ccataaataa tgcgccgact
     4141 ttttgtagat aatgttccgg gacatgggcc atttgcagac cgagaaaatc tttaatctgc
     4201 atggtaccga tggtgatccc gatacccgag gtgaaaccta aggtgacgga aaccggaata
     4261 tactcaatca gacgaccaaa gcgtgccagc cccatcagaa tcaaaaagat tcccgacaac
     4321 aaggtcgcaa ccagcagtcc tgccagtcca aactgttgcg aaacgggata gagaattacc
     4381 acaaatgccg cagtcggacc ggaaacgcta aagcgtgacc cacccgttag agcaatgaca
     4441 atccccgcaa cagctgcggt atataaaccg tactggggtg ccacaccact accaatagcc
     4501 aacgccatcg ccagcgggat agcaataatc ccgacggtta tcccggcaat caggtcacgg
     4561 gtaaaccgtg cggcagtata tttttctttc cagcaagcgt cgatcagagc gcggaaaggc
     4621 atcacatgtg aggaaaatat tttgttcaca ataatgtttc atccgtgagc gcatcatctg
     4681 tcaactaaat ggcaggtgaa ggaggcatag gtcatacaaa tggatattac agacaaaaaa
     4741 acccgccgca gcgggtcttt gagccgggtt cgattagtgt tcgaacatgg cagagatcga
     4801 ttcttcgttg ctgatacgac gaatcgcttc ggccagcata cctgacaggg tcagagtacg
     4861 cacgttcggc agtgatttga tttcatcgct cagcggaatg gtatcgcaga caacgacttc
     4921 atcaattaca gagttacgca ggttgttcgc cgcgttgcca gagaagatcg ggtgagtcgc
     4981 gtacgcaaat acacgtttag caccacgttc tttcagcgct tcagcagctt tacacagcgt
     5041 accgccagtg tcgatcatat catcgaccag tacgcagtca cgacctgcaa cgtcaccgat
     5101 gatatgcatc acctgggaaa cgttcgcacg cggacgacgt ttgtcgatga ttgccatatc
     5161 ggtatcgttc agcagcttag cgatagcgcg ggcacgcaca acgccgccga tgtccggaga
     5221 aaccacaatt gggttatcca gattcagctg cagcatgtct tccagcagga tcgggctacc
     5281 aaatacgtta tcaaccggaa cgtcgaagaa accctgaatc tgttcagcgt gcagatccac
     5341 tgtcagcaca cggtcaacac cgacgctgga gaggaagtct gcaaccactt tcgcagtgat
     5401 tggtacacga gcggaacgga cgcgacggtc ctggcgcgca tagccaaagt aggggataac
     5461 agcggtgata cgacctgcgg aagcacgacg cagggcatca accataacga ctaattccat
     5521 caggttgtcg ttagtagggg cacaagtgga ctggatgatg aaaatatcac caccgcgtac
     5581 attttcgtta atttgtacgc tgacttcgcc gtcgctaaag cgacctacag cggcgtcgcc
     5641 gagtgaagtg tacaggcggt tggcaatacg ttgtgctagt accggggtgg cgttaccagc
     5701 aaaaagcttc atatcaggca cgagaagaac ctcaggcatg cgtccattgg tggaaagaat
     5761 ctgccgaaaa ctgtgcgggc caggcatgat cctttccagg cggtgtatta aagagcgcga
     5821 tgcaacgtct ggaacaaggt gacgttgtca ccgaaactca gcttgcccgg cttaaagcat
     5881 ggctctgtgc aatgaggaaa gattaacgcc tttcgccaca aagccattga gccattccgg
     5941 ggcttgctct agcacctggc gggcttcaga ctctgtatca aattcagcaa agacacaggc
     6001 ccctgtccca gtcaggcgcg acggggcgta ttctaacagc caggaaagca ccgcatcaac
     6061 ctcgcgaaaa cgttttcttg cgataacctc gcaatcattg ctgaattcac attttagcaa
     6121 cgtttctatt gacctttttg gcgtattgcg cgggagttca ggatctttaa aaatcaccgg
     6181 agtcggaata cttacaccag ggtgcgccac cagataccac ttctctggcg gatccaccgg
     6241 cgttagtatt tcaccaacgc cttcggcaaa cgcggcatgc ccccgaacaa agacaggaac
     6301 atctgcgccc agcgtcagcc ccatttccgc cagctcatcc atgcttagcc cgcattgcca
     6361 gagatgattt aatgccacca ggaccgtcgc ggcattggat gaaccaccgc cgagaccgcc
     6421 gcccatcggc aaacgcttgt caatgctgat attcgcaccg cttcccgtcg gaagacgccc
     6481 gctgtctgcc gcagttttca tcaacaaccg cgctgcgcga acgatcaggt tatcttcatg
     6541 ttccacgcct tcaacgggcg ttaacagacg aatatcccca tcgtcacgaa gctcaatgct
     6601 gatggtgtcg ccgtaatcaa gaaactgaaa cagcgtttgc agcgtgtggt aaccatccgc
     6661 acgctgaccg gtaatgtata aaaacagatt aagttttgcc ggagagggcc actgtgtccg
     6721 cattatttca ctatccagtt atccattttt aacttgatgc gttgaccacc gtcggtgagt
     6781 tccatattgg ctggcatcgc aggttgcgtt ttggtgtcat aaccaccata aacaaccttc
     6841 cagtttttgc cattctggct gtaggtaatt tcgctcaggc ggtactggtc gtccagtttg
     6901 tagtcggttg catcacccgg taaacctaaa atccactggc gcaagctgtt gagcggaatt
     6961 ggcattccgg tcaatttgcc aatcatctct tcggcgtcat cggcggtata acgctgacct
     7021 ttattgtcga ctaactgcac gttacccggt tgagcattca gctccagttc cgtgctgccc
     7081 aatgggttag tgagcagcag acggtagcga tcctggccgg tttgctgcca gaaaaagcgg
     7141 gcgtacactt tttgttggtc agaaatataa gcgaacgcgc cgcgagtctg atactgatta
     7201 agattgcgca cgtcttgctg atgctgacgc cattgtggcg aatccgggct tttgccagga
     7261 cctttgggcg tggtaacgga acaggcagtg agcacaagag cagccagcgg tagcaggcgg
     7321 ataagacgaa aatcgggcag gggcatagtg atgacaagtc cttgagatac gttgcagtta
     7381 taacccttaa tgctagcgtt accgtccgct atcgtctatg ttcaagttgt cttaattgcc
     7441 agaatctaac ggctttcggc aattactcca aaagggggcg ctctctttta ttgatcttac
     7501 gcatcctgta tgatgcaagc agactaaccc tatcaacgtt ggtattattt cccgcagaca
     7561 tgaccctttt agcactcggt atcaaccata aaacggcacc tgtatcgctg cgagaacgtg
     7621 tatcgttttc gccggataag ctcgatcagg cgcttgacag cctgcttgcg cagccgatgg
     7681 tgcagggcgg cgtggtgctg tcgacgtgca accgcacgga actttatctt agcgttgaag
     7741 agcaggataa cctgcaagag gcgttaatcc gctggctttg cgattatcac aatcttaatg
     7801 aagaagatct gcgtaaaagc ctctactggc atcaggataa cgacgcggtt agccatttaa
     7861 tgcgtgttgc cagcggcctg gattcactgg ttcttgggga gccgcagatc ctcggtcagg
     7921 ttaaaaaagc gtttgccgat tcgcaaaaag gtcatatgaa ggccagcgaa ctggaacgca
     7981 tgttccagaa atctttctct gtagcgaaac gcgttcgcac tgaaacagat atcggtgcca
     8041 gtgctgtgtc tgtcgctttt gcggcttgta cgctggcgcg gcagatcttt gaatcgctct
     8101 ctacggtcac agtgttgctg gtaggcgcgg gcgaaaccat cgagctggta gcgcgtcatc
     8161 tgcgcgaaca taaagtacag aagatgatta tcgccaaccg cactcgcgaa cgtgcccaaa
     8221 ttctggcaga tgaagttggc gcggaagtga ttgccctgag tgagatcgac gaacgtctgc
     8281 gcgaagccga tatcatcatc agttccaccg ccagcccgtt accgattatc gggaaaggca
     8341 tggtggagcg cgcattaaaa agtcgtcgta accaaccaat gctgttggtg gatattgccg
     8401 ttccgcgcga tgttgagccg gaagttggca aactggcgaa tgcttatctt tatagtgtgg
     8461 acgatctgca aagcatcatt tcgcacaacc tggcgcagcg taaagccgca gcggttgagg
     8521 cggaaactat tgtcgctcag gaaaccagcg aatttatggc gtggctgcga gcacaaagcg
     8581 ccagcgaaac cattcgcgag tatcgcagcc aggcagagca cgttcgcgat gagttaaccg
     8641 ccaaagcgtt agcggccctt gagcagggcg gcgacgcgca agccattatg caggatctgg
     8701 catggaaact gactaaccgc ttgatccatg cgccaacgaa atcacttcaa caggccgccc
     8761 gtgacgggga taacgaacgc ctgaatattc tgcgcgacag cctcgggctg gagtagcagt
     8821 acatcatttt ctttttttac agggtgcatt tacgcctatg aagccttcta tcgttgccaa
     8881 actggaagcc ctgcatgaac gccatgaaga agttcaggcg ttgctgggtg acgcgcaaac
     8941 tatcgccgac caggaacgtt ttcgcgcatt atcacgcgaa tatgcgcagt taagtgatgt
     9001 ttcgcgctgt tttaccgact ggcaacaggt tcaggaagat atcgaaaccg cacagatgat
     9061 gctcgacgat cctgaaatgc gtgagatggc gcaggatgaa ctgcgcgaag ctaaagaaaa
     9121 aagcgagcaa ctggaacagc aattacaggt tctattacta ccaaaagatc ctgatgacga
     9181 acgtaacgcc ttcctcgaag tccgtgccgg aaccggcggc gacgaagcag cgctgtttgc
     9241 aggcgatctg ttccgtatgt acagccgtta cgccgaagcc cgccgctggc gggtagaaat
     9301 catgagcgcc agcgagggtg aacatggtgg ttataaagag atcatcgcca aaattagcgg
     9361 tgatggtgtg tatggtcgtc tgaaatttga atccggcggt catcgcgtgc aacgtgttcc
     9421 tgctacggaa tcgcagggtc gtattcatac ttctgcttgt accgttgcgg taatgccaga
     9481 actgcctgac gcagaactgc cggacatcaa cccagcagat ttacgcattg atactttccg
     9541 ctcgtcaggg gcgggtggtc agcacgttaa caccaccgat tcggcaattc gtattactca
     9601 cttgccgacc gggattgttg ttgaatgtca ggacgaacgt tcacaacata aaaacaaagc
     9661 gaaggcgctt tcagtgctcg gtgctcgcat ccacgctgct gaaatggcaa aacgccaaca
     9721 ggccgaagcg tctacccgtc gtaacctgct ggggagtggc gatcgcagcg accgtaaccg
     9781 tacttacaat ttcccgcagg ggcgcgttac cgatcaccgc atcaacctga cgctctaccg
     9841 cctggatgaa gtgatggaag gtaagctgga tatgctgatt gaaccgatta tccaggaaca
     9901 tcaggccgac caactggcgg cgttgtccga gcaggaataa tggaatatca acactggtta
     9961 cgtgaagcaa taagccaact tcaggcgagc gaaagcccgc ggcgtgatgc tgaaatcctg
    10021 ctggagcatg ttaccggcag agggcgtact tttattctcg cctttggtga aacgcagctg
    10081 actgacgaac aatgtcagca acttgatgcg ctactgacac gtcgtcgcga tggtgaaccc
    10141 attgctcatt taaccggggt gcgagaattc tggtcgttgc cgttatttgt ttcgccagcg
    10201 accttaattc cgcgcccgga tacggagtgt ctggtggagc aggcactggc gcggttgcct
    10261 gaacaacctt gccgtattct cgatctcggg acgggtaccg gggcgattgc gcttgcgctg
    10321 gctagcgagc gcccggactg tgaaattatc gctgtagatc gtatgcctga tgctgtctcc
    10381 ctggcacaac gtaatgccca gcatctggcg atcaaaaata tccacattct gcaaagcgac
    10441 tggtttagcg cgctagccgg gcagcagttt gcgatgattg tcagcaatcc gccgtatatt
    10501 gacgagcagg acccacatct tcaacaaggc gatgtccgct ttgagccgct cactgcgctg
    10561 gttgcggcag acagtggaat ggcagacatc gtgcatatca tcgaacagtc gcgtaacgcg
    10621 ctggtatccg gcggctttct gcttctggaa catggctggc agcagggcga agcggtgcga
    10681 caagcattta tcctcgcggg gtatcatgac gtcgaaacct gccgtgacta tggtgataac
    10741 gagcgcgtaa cgctcggccg ctattatcaa tgacaagttt ttctacactg cttagtgttc
    10801 atcttattag tatcgcgctt tctgttgggc tattaacctt acgtttctgg ctacgttatc
    10861 agaagcatcc acaggcattt gctcgctgga cgcgcattgt gccgccggtt gtcgatacgg
    10921 tgttattgtt aagcggcatt gcgttgatgg ctaaagcgca catcctgcca ttttccgggc
    10981 aggcacagtg gctgactgaa aagctgtttg gagttatcat ttatatcgtt ttgggtttta
    11041 ttgcactcga ttatcgtcat atgcacagtc agcaggcgcg cattattgcc ttcccgctgg
    11101 cgttggtggt gctgtacatc atcattaaac tcgccaccac aaaagtaccg ttactggggt
    11161 aagtcatgag atcgttagct gatttcgaat ttaataaagc gccattgtgc gaaggcatga
    11221 tcctggcttg cgaagcaatc cgccgcgatt ttccctcgca agatgtttac gacgaactgg
    11281 agcgtctcgt tagtctggcg aaggaagaaa tcagccagct tctgccttta gaagagcagc
    11341 tggaaaaact gatcgcgctg ttttacggcg actggggatt taaagcctca cgcggcgttt
    11401 atcgtctttc cgatgcatta tggctggacc aggtgttaaa gaatcgacag ggcagtgcgg
    11461 tatcattagg tgcggtttta ttatgggtcg cgaatcgtct cgatttgccg ctgctgccgg
    11521 tgattttccc tacgcagctg atattgcgca ttgaatgtcc ggatggcgaa atttggctga
    11581 ttaatccttt taacggtgaa tcgttaagcg aacatatgct ggacgtatgg ttaaagggaa
    11641 atatcagccc gtcggcggaa ctgttttatg aaaaccttga tgaagctgat aacattgagg
    11701 taatccgcaa attgctggat acactcaaag ccatcgttga tggaagaaaa tcagatggag
    11761 ctggcgttac gcaccagcga agctttatta caattcaacc ctgaagatcc ctatgaaatt
    11821 cgcgatcgcg ggttgattta tgcgcaactg gattgcgaac acgttgcgtt gaacgattta
    11881 agttatttcg ttgaacagtg tccggaagac ccgatcagcg aaatgatccg tgcgcaaata
    11941 aataacatcg cgcataaaca tattgtgctg cattaattaa tcgacatttt actcaagatt
    12001 aaggcgatcc tatgaaacaa aaagtggtta gcattggcga catcaacgta gcaaatgacc
    12061 tgccgttcgt actgtttggc ggtatgaacg tgctggaatc tcgcgatctg gcgatgcgca
    12121 tttgcgagca ctacgtaact gtgacccaga aactgggtat cccttacgtg ttcaaagcct
    12181 cttttgacaa agccaaccgt tcctccatcc actcttatcg tggaccgggc ctggaagaag
    12241 ggatgaaaat cttccaggag ttgaagcaga cttttggcgt gaaaattatc accgacgttc
    12301 acgaaccaag tcaggcacag cccgttgctg atgtcgtgga tgtgattcag ttgccggcgt
    12361 ttcttgctcg ccagactgac ctggttgaag ccatggcgaa aaccggtgcg gtaattaacg
    12421 tcaagaaacc acagtttgtc agcccgggac agatgggtaa tatcgttgat aaattcaaag
    12481 aaggcggcaa cgaaaaagtg attctttgcg atcgtggtgc taacttcggc tatgacaacc
    12541 tggttgtcga tatgctgggc ttcagcatta tgaagaaagt gtctggtaac tcgccggtga
    12601 ttttcgacgt gacccacgca ctgcaatgcc gcgatccgtt tggcgcagct tccggtggtc
    12661 gtcgtgctca ggtggctgag ctggcacgag ccggtatggc ggtaggtctg gcggggctgt
    12721 ttattgaagc gcatccggat ccggaacatg cgaaatgtga tggtccatcc gcgctgccgc
    12781 tggctaaact ggaaccgttc ctcaagcaga tgaaagcgat tgatgatctg gtgaaaggtt
    12841 tcgaagaact ggataccagc aagtaatctt ttttgcttga aaaataaagt cttagcgttc
    12901 tgcgttaaga cttttttcat gggtgccaga tacaaaaaag gccgcag
//
LOCUS       DADLSB010000108        12841 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.108, whole genome shotgun sequence.
ACCESSION   DADLSB010000108 DADLSB010000000
VERSION     DADLSB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.108"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(221..2290)
                     /gene="glyS"
                     /locus_tag="JN331_002920"
     CDS             complement(221..2290)
                     /gene="glyS"
                     /locus_tag="JN331_002920"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY7261018.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2300..3211)
                     /gene="glyQ"
                     /locus_tag="JN331_002921"
     CDS             complement(2300..3211)
                     /gene="glyQ"
                     /locus_tag="JN331_002921"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY7261019.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3306..3605)
                     /locus_tag="JN331_002922"
     CDS             complement(3306..3605)
                     /locus_tag="JN331_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261020.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3780..4775
                     /locus_tag="JN331_002923"
     CDS             3780..4775
                     /locus_tag="JN331_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7261021.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKSVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4817..5254)
                     /locus_tag="JN331_002924"
     CDS             complement(4817..5254)
                     /locus_tag="JN331_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY7261022.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5300..5641)
                     /locus_tag="JN331_002925"
     CDS             complement(5300..5641)
                     /locus_tag="JN331_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261023.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIDVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5810..7264)
                     /gene="xylB"
                     /locus_tag="JN331_002926"
     CDS             complement(5810..7264)
                     /gene="xylB"
                     /locus_tag="JN331_002926"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY7261024.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAMVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7336..8658)
                     /gene="xylA"
                     /locus_tag="JN331_002927"
     CDS             complement(7336..8658)
                     /gene="xylA"
                     /locus_tag="JN331_002927"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAY7261025.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            9024..10016
                     /gene="xylF"
                     /locus_tag="JN331_002928"
     CDS             9024..10016
                     /gene="xylF"
                     /locus_tag="JN331_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7261026.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            10094..11635
                     /gene="xylG"
                     /locus_tag="JN331_002929"
                     /pseudo
     CDS             10094..11635
                     /gene="xylG"
                     /locus_tag="JN331_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
     gene            11613..12794
                     /gene="xylH"
                     /locus_tag="JN331_002930"
     CDS             11613..12794
                     /gene="xylH"
                     /locus_tag="JN331_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY7261027.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMCRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
BASE COUNT         3238 a         3247 c         3099 g         3257 t
ORIGIN      
        1 tctgcctttc ggtgaatacc tgagacaaac agtctcaagc acccgtggct attctagctt
       61 aataagtttg tttcttctcc ttgatataat cctaaaaaaa tctcataaaa ttaatatatg
      121 agataatctt tattcagcag aagattatta aaggttgctg tattatttag cgataataaa
      181 aaaagcctgc cagatggcag gctttttaat aacggcgtta ttactgcaac agcgaaatat
      241 ccgcaacgcg caggaacagt tcgcgcagtt tctccagcat ggtcagacgg ttgagacgca
      301 attctttgtc atcaaccatg accatcactt tatcgaagaa agcatcaacc ggctcacgca
      361 gctctgccag ttcgaccagc gcatcctggt aacgaccttc cgcaaagtac ggctccagct
      421 tgtcacgtag caccacaacc tgcatcgcca gtttaatttc ttccggctct ttcagggtag
      481 aggcattcac gcgatcgctc agcacttcgt cagatttcgc caggatgtta gatacacgtt
      541 tgttcgccgc cgccagtgct gcagctgctt ccagggtacg gaagtgcgat accgctttca
      601 tgcgggcatc gaaatcagcc ggacgagtcg gacgacgcgc cagtaccgcc tggatggtgt
      661 caacggtata accttcgtcc tgataccagg cgcggaagcg accgagcata aagtcgataa
      721 catcatcaac tacgttggca ttagtcagct tatcgccata cagacgcacc gcctcttcgg
      781 tcagcgtttg cagatcaagg ttgaggttct tctcaacgat aattcgcagc acgccaagtg
      841 cggcacgacg cagcgcaaac gggtctttgt cgcctttcgg atgctgaccg ataccgaaga
      901 tacccgccag agtatccatc ttatcagcaa tcgccagcgc acaagctacc gggttggacg
      961 gcaggtcatc accggcaaag cgcggctgat actgctcatt cagcgcgacg gctacgtctt
     1021 ccgcttcgcc atcgtgacgc gcgtagtgca tccccataac gccttgggtg tcggtgaact
     1081 cgaagaccat gttggtcatc aggtcacact tagacagcag gcccgcacgg gttgcgtggt
     1141 taacgtcagc gccaatctgt tcggcgatcc agccagccag cgcctggatg cggtcagtct
     1201 tgtcacgcag cgtacccagc tgttgctgga acagcacggt ttgcaggcgc ggcaggttat
     1261 cttccagacg ttttttgcgg tcggtgttga agaagaactc ggcatccgcc agacgtggac
     1321 gaacgacttt ctcgttacca gagataattt gctgcggatc tttcgattcg atgttggcaa
     1381 cgaagataaa gttcggcagc agtttgccgt cgttcgcata caccgggaag tacttctggt
     1441 cacctttcat ggtgtaaacc aaggcttcag ccggcaccgc gaggaatttc tcttcgaatt
     1501 ttgcggtcag aacgaccggc cactccacca gcgaagccac ttcttccagc aggctttcgc
     1561 ttaagtcagc gttaccgcca atcttacgcg ctgcttcttc ggcatcggct ttaatcttcg
     1621 ctttacgttc ttcgtaatcg gcgatgactt tcccgcgctc acgcagaatt tccggatact
     1681 gatcggcgtt gtcgatggtg aattccggtt cgcccataaa gcggtggccg cgaatcacgc
     1741 gatcggactg aatgcccaga atggttgccg gaatgacttt gtcgcccagc agcagggtca
     1801 cggtgtgcac cggacgcacg aagtgcacgt cgcttgcacc ccagcgcatc agtttcggga
     1861 tcggcagttt cgccagcgaa gtcgcaacca tattcggcag cagtgcttcg gtgctttcgc
     1921 ccttcacatg ggcgcgatac agcagccatt cgcctttatc ggtagtcaga cgctcagcct
     1981 ggtcaacggt aataccacaa ccacgcgccc aaccttctgc cgctttgctc ggttttcctt
     2041 cagcgtcgaa cgcctgggca atcgccgggc cgcgtttttc gatttcacga tctggttgtg
     2101 cttccgccag gttagctact ttcagcgcca gacgacgcgg agcagcaaac cattgaacgg
     2161 tgccgtgtgc gaggcctgcg ttatccagct ccgcagtaaa gttcgcagca aaggactctg
     2221 ccaggctgcg cagtgctttt ggtggcagct cttcagtgcc gatttccacc agaaaagttt
     2281 tctcagacat agccgcctct tacttatctt tgttgcacat cgggaagccg agggcttcac
     2341 gggaagcgta atatgcttct gccactgctt tggtcagggt gcgaatgcgc agaatgtagc
     2401 gctgacgctc ggtgacggag atggctttac gcgcatccag caggttgaag ctgtgggcgg
     2461 ctttcagaat acgctcgtag gctggcagcg gcagcggatt ttccagcgcc agcagctgct
     2521 gcgcttcttt ctcgtactgc tcaaagcagg tgaacaggaa gtccacatcc gcgtattcga
     2581 agttgtaagt ggactgctcc acttcgttct gatggaacac gtcgccgtag gtggttttac
     2641 ccagcgggcc gtcgctccag accaggtcgt aaacgctgtc tacgccctga atgtacatgg
     2701 ccagacgttc cagaccgtag gtgatctcgc cggtaaccgg tttacactcc agaccaccaa
     2761 cctgctggaa gtaagtgaac tgcgtcactt ccatgccgtt tagccacact tcccagccca
     2821 gtccccaggc ccccagagtc gggttttccc agttatcttc cacgaaacgg atgtcgtgaa
     2881 tagtcgggtc catgcccagc tctttcagag aaccgaggta cagctcctga atattgtccg
     2941 gcgatggctt aatgaccacc tggaactgat agtagtgctg taaacggttg gggttttcgc
     3001 cgtagcgacc atcggtcggg cgacgagaag gctgaacata agcagccgcc atcggttctg
     3061 gccccagcgc gcgcagacag gtcattgggt gagaggttcc cgcgccgact tccatgtcca
     3121 atggttgaac aatggtgcag ccctggcgag cccagtaatc ctgtaaggtc aggatcaagc
     3181 cctggaaggt cctggtatca aacttttgca tattatttcg tgctggatac gtgtggattt
     3241 aaaggaaggg atcagtatac ccgctggatg gaagatatac agtacgaaac gggaaaaagc
     3301 agggcttaac gcatggaaag atgcaaaaaa tggccatctg cgtcaaaaga acagacaaac
     3361 tgctctttac ggaaggtata accgcgcatt tcatagcttc cctggaactg ttcgaaggcg
     3421 gtgacgtcga ttttttgctc gccggtgtta taacgttttg ccgcctgatc cttgcataac
     3481 gcttccatat tcaacgaacg cagaggatca acctttaccc gttgtgcttt ctgcacgggt
     3541 gacggtgtag aacaacccac tagcaccata agcgccattg ccggaaagaa agcgttcatc
     3601 atcattaaat taccgtctgg tttgcaggtg agtcttatta tttatatcgg tatagtggct
     3661 gatatcttac gaatttcctt aagcctctat aggctgtagt gataaaactc agattattcc
     3721 agtgcgccaa ccaaatgatt tatcggtcag acttttttta accagataca gagacacaga
     3781 tgcagcccaa aatttactgg attgataacc tgcgagggat agcgtgttta atggtggtga
     3841 tgattcacac cactacctgg tatgtgacca atgctcatag tgttagcccc gtcacatggg
     3901 atatcgccaa tgttctgaat tctgcctctc gtgtcagcgt gccgctattt ttcatgattt
     3961 ccggctatct cttttttggc gaacgcagcg cccagccgcg ccatttcttg cgtatcggct
     4021 tatgtctgat tttttatagc gcaatcgcac tgctctacat tgcgctgttt acctccatca
     4081 atatggagtt agcgctgaaa aacctgctgc aaaagtcagt gttttaccac ttgtggtttt
     4141 tcttcgcgat tgcggtgatt tatctggttt caccgctgat tcaggtgaag aacgtcggcg
     4201 gaaaaatgtt gctggtgcta atggtggtga ttggcattat cgctaaccca aacacagtgc
     4261 cgcagaaaat tgacggtttt gaatggctgc caattaactt atatatcaat ggcgatactt
     4321 tttactacat tctgtatggc atgttgggcc gcgctatagg gatgatggac acacagcata
     4381 aagcactgtc gtgggtgagc gccgcgctgt ttgcgactgg ggtttttatt atctcacgcg
     4441 ggacattata tgaattgcag tggcgcggaa attttgccga tacctggtat ctttactgtg
     4501 ggccgatggt ttttatctgc gcaatcgcgc tattgactct ggttaaaaac acgctggata
     4561 cgcgtaccat tcgcggactt ggcttaatct cccgccattc attgggtata tacggattcc
     4621 acgccttgat tatccatgcg ctgcgcaccc ggggaattga gcttaaaaat tggccaatac
     4681 tggatattat ttggatcttt tgcgcgacgt tggcagcgag tttgttactt tctatgctgg
     4741 tacaacgaat cgacagaaac agattagtga gttaagtaaa agcccgttca cattggactg
     4801 accgggctta cgtgaattat tcgctgtatt cttcctgggt aacctgtgtt tcttttggcg
     4861 ggtttattcc ggcatcacga atattcttct gcaccgctac tgcaccaaac aagcttaaga
     4921 agaaagccag tccataaaaa cctttttcac tgagtaataa tgtcgcattc cacagaccta
     4981 ccatcagtag tgcaacagag ataataaaga cagtcaggca ggtcatataa taaatggaag
     5041 tggtcggtat gccttcatac ttgtcccgaa cggtcttttg ataagacgca gcagaaaaca
     5101 gtcctaacac cagtacggca aaataatacc ctttttcatt taactgcatc tctgcattcc
     5161 atagccctaa caggtaggta acgataccac caacgagagc tatccatgac acaatactaa
     5221 aggccggtga ataggttgat attttgttgt ccatcacagt attcctttca ttcctgaatg
     5281 tgtaagagct ttatgttgct cataccgagc gaaacaaacg catttgacca aacaagcaga
     5341 caaaaaaggc gatggggtag atcaccattt cataagcatt gataggtgca tttaaaacat
     5401 cgataaacaa gagaccgata gtgattaacg caactaactg gcagatatgg gtaaaaaaat
     5461 tatccagttg cccgagtttt tctgcgcgaa gatatgcata gttaccaaaa gttgctgtca
     5521 ttaacacgcc aagaaaatag ccttttccac ttagcaatgg gcatgagatc catagcccaa
     5581 ccagataaac cagcgccccg acaacaaata atagttttgc cacagttttt gaggttttca
     5641 taacgatctc catatctacc agcgatacat tacgagtaac caacgaaaga caaaactgaa
     5701 aaatgccatt aacaaatgat tttcagaata aattcatact aaatattaat taattagtga
     5761 gatatataga tgtgaattat cccccacccg gtcaggcagg ggataacgtt tacgccatta
     5821 atggcagaag ttgctgatag aggcgacgga acgtttcacg tcgtggctga taagcggcat
     5881 aacgctgcgc atctggtaga tgcgactgtt ctaacggtag ctgcggcaac aactcaatga
     5941 gcgatttctc tggattcgcc gcaatctgcg ccagccttgc tgcgcccagt gctggcccca
     6001 catccccccc cgtacggtaa tcgagctgct gaccgctgat atccgccagc atctgacgcc
     6061 agtactcact acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg
     6121 catgcacgac atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg
     6181 ccagttcatt ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat
     6241 tattgtgtgg tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcggcacttt
     6301 catcagcctg ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg
     6361 cgacccaatc cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg
     6421 gtagcgcatg gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga
     6481 cagcaaaata gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc
     6541 ccacaccaac tgcaccagct gcattgtcgc caccgcctgc gacaactggc accatcgcca
     6601 taccccacgc tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata
     6661 atgcgggcat ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac
     6721 gctttgcgac atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc
     6781 ccgtcatacg cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata
     6841 tctccggctc atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca
     6901 ggttgccggt aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg
     6961 cacagcgccc gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta
     7021 aggttgctcc atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct
     7081 gatcgcccag agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt
     7141 ccgaccaaag tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa gcaaccacct
     7201 caccctgctc gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat
     7261 acatatcgat cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga
     7321 ctgcacagtt agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc
     7381 ctggcgacca ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc
     7441 tgccagtgac atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata
     7501 acgctgcgcg atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag
     7561 cgccagtgcc atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt
     7621 actttgacga cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt
     7681 gagaatttca tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc
     7741 ccagcccagt tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat
     7801 ggcggtggct atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat
     7861 gttcagttta atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc
     7921 atcgtaatca tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtacc
     7981 ctggaagccg attttatgtt tatgctcaac caccatctgc ataaagcggc ccagttgttc
     8041 acgctcctga cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac caccccacag
     8101 gacatagttt tcaccgccca atttatgggt tgcttccatc gctgtaacaa cttgcgttgc
     8161 cgcccagctg aagacttcag gatctgggtt cgtcgccgca cccgcgccat agcgagggtt
     8221 tgtaaagcag ttagcggttc cccacagcag cttcacgccg ctctcttctt gcttggctgc
     8281 caggacatca accatttgcg caaaattatt gatgtactct tttaacgacg cgccctcagg
     8341 ggaaacatcc acatcgtgga agcaataaaa tggcacatgt aacttgtgga aaaactcaaa
     8401 tgcgacatct gctttacgct tcgccaacgc cagtgcctca ccaggctgct gccacggacg
     8461 attaaacgcc cccacaccaa acatgtccgc cccgttccag cagaaggtgt gccagtagca
     8521 ggcggcaaaa cgcaagtgct cttccatacg cttacccaac accagttcgt cgggattgta
     8581 gtgacggaat gctaacgggt ttgaggattt tgagccttca taacgaacgc gatcgagctg
     8641 gtcaaaatag gcttgcatat tgaactccat aatcaggtaa taccgcgggt gatggatgat
     8701 gtcgtaatat tgggcactcc ctttcagttg ctcaattatg ttatttcaca ctgctattga
     8761 gataattcac aagtgtgcgc tcgctcgcaa aataaaatgg gatgatgaaa ctgggtaatt
     8821 cctcgaagat aaaaatgcaa taagtacaat tgcgcaacaa aagtaagatc tcggtcataa
     8881 atcaagaaat aaaccaaaaa tcgtaatcga aagataaaaa tctgtaattg ttttcccctg
     8941 tttagttgct aaaaattggt tacgtttatc gcggtgattg ttacttatta aagctgtcct
     9001 ctaactacag aaggccctac accatgaaaa taaagaacat tctactcacc ctttgtacct
     9061 cacttctgct taccaacgtt gcggcacacg ccaaagaagt caaaataggt atggcgattg
     9121 atgatctccg tcttgaacgc tggcaaaaag atcgagatat ttttgtgaaa aaggcagaat
     9181 ctctcggtgc gaaagtattt gtacagtctg caaatggcaa tgaagaaaca caaatgtcgc
     9241 agattgaaaa catgattaac cggggtgtcg atgttcttgt cattattccg tataacggtc
     9301 aggtattaag taacgttgta aaagaagcca aacaagaagg tattaaagta ttagcttacg
     9361 accgtatgat taacgatgcg gatatcgatt tttatatttc tttcgataac gaaaaagtcg
     9421 gtgaactgca ggcaaaagcc ttggtcgata ttgttccgca aggtaattac ttcctgatgg
     9481 gcggctcgcc ggtagataac aacgccaagc tgttccgcgc cggacaaatg aaagtgttaa
     9541 aaccttacgt tgattctgga aaaattaaag tcgttggtga ccaatgggtt gatggctggt
     9601 taccggaaaa cgcattgaaa attatggaaa acgcgctaac cgccaataat aacaaaattg
     9661 atgctgtagt tgcctcaaac gatgccaccg caggtggggc aattcaggca ttaagcgcgc
     9721 aaggtttatc agggaaagta gcaatttccg gccaggatgc ggatctcgca ggtattaaac
     9781 gtattgctgc cggtacgcaa actatgacgg tgtataaacc tattacattg ctggcaaata
     9841 ctgccgcaga aattgccgtt gaattgggca atggtcagga gccaaaagca gatacctcac
     9901 tgaataatgg cctgaaagat gtcccctccc gcctcctgac accgatcgat gtgaataaaa
     9961 acaacatcaa agatacggta attaaagacg gattccacaa agagagcgag ctgtaagcgt
    10021 tacgccccgg cgcggagcgg gggcgtgatt tctctccatg ccgcgtgaat gaattggctt
    10081 aggtggagtc gttatgcctt atctacttga aatgaagaac attaccaaaa ccttcggcag
    10141 tgtgaaggcg attgataacg tcagcttgcg gttgaatgct ggcgaaatcg tctcactctg
    10201 tggggaaaat gggtctggta aatcaacgct gatgaaagtg ctgtgtggta tttatcccca
    10261 tggctcctac gaaggcgaaa ttatttttgc gggagaagag attcaggcga gtcacatccg
    10321 cgataccgaa cgcaaaggta tcgccatcat tcaccaggaa ttggccctgg tgaaagaatt
    10381 gaccgtgctg gaaaatatct tcctgggtaa cgaaataacc cacaatggca ttatggatta
    10441 tgacctgatg acgctacgct atcagaagct gctcgcacag gtcagtttat ccatttcacc
    10501 tgatacccgc gttggcgatt tagggcttgg gcaacaacaa ctggttgaaa ttgccaaggc
    10561 acttaataaa caggtgcgct tgttaattct cgatgaaccg acagcctcat taactgagca
    10621 ggaaacgtcg gttttactgg atattattcg cgatctacaa cagcacggta tcgcctgtat
    10681 ttatatttcg cacaaactca acgaagtcaa agcgatttcc gatacgattt gcgttattcg
    10741 cgacggacag cacattggta cgcgtgatgc tgccggaatg agtgaagacg atattatcac
    10801 catgatggtc gggcgagagt taaccgcgct ttaccctaat gaaccacata ccaccggaga
    10861 tgaaatatta cgtattgaac atctgacggc atggcatccg gtcaatcgtc atattaaacg
    10921 agttaatgat gtctcgtttt ccctgaaacg tggcgaaata ctgggtattg ccggactcgt
    10981 tggtgccgga cgtaccgaga ccattcagtg cctgtttggc gtgtggcccg gacaatggga
    11041 aggaaaaatt tatattgatg gcaaacaggt agatattcgt aactgtcagc aagccatcgc
    11101 ctaggggatt gcgatggtac ccgaagacag aaagcgcgac ggcatcgttc cggtaatggc
    11161 ggttggtaaa aatattaccc tcgccgcact caataaattt accggtggca ttagccagct
    11221 tgatgacgcg gcagagcaaa aatgtattct ggaatcaatt cagcaactca aagttaaaac
    11281 gtcgtccccc gaccttgcta ttggacgttt gagcggcggc aatcagcaaa aagcgatcct
    11341 cgcccgctgt ctgttactta acccgcgcat cctcattctt gatgaaccta ccaggggtat
    11401 tgatattggc gcgaaatatg agatctacaa attaattaac caactcgtcc agcagggtat
    11461 tgccgttatt gtcatctctt ccgaattacc tgaagtgctc ggccttagcg atcgtgtact
    11521 ggtgatgcat gaagggaaac taaaagccaa cctgataaat cataacctga ctcaggagca
    11581 ggtgatggaa gccgcattga ggagcgaaca tcatgtcgaa aagcaatccg tctgaagtga
    11641 aattggccgt accgacatcc ggtggctttt ctgggctgaa atcactgaat ttgcaggtct
    11701 tcgtgatgat tgcagctatc atcgcaatca tgctgttctt tacctggacc accgatggtg
    11761 cctacttaag cgcccgtaac gtctccaacc tgttacgcca gaccgcgatt accggcatcc
    11821 tcgcggtagg aatggtgttc gtcataattt ctgctgaaat cgacctttcc gtcggctcaa
    11881 tgatggggct attaggtggc gtcgcggcga tttgtgacgt ctggttaggc tggcctttgc
    11941 cacttaccat cattgtgacg ttggttctgg gactgcttct cggtgcctgg aacggatggt
    12001 gggtcgcgta ccgcaaagtc ccttcattta ttgtcaccct cgcgggcatg ttggcatttc
    12061 gcggcatact cattggcatc accaacggca cgactgtatc ccccaccagc gccgcgatgt
    12121 cacaaattgg gcaaagctat ctccccgcca gtaccggctt catcattggc gcgcttggct
    12181 taatggcttt tgttggttgg caatggcgcg gaagaatgtg ccgtcaggct ttgggtttac
    12241 agtctccggc ctctaccgca gtagtcggtc gccaggcttt aaccgctatc atcgtattag
    12301 gcgcaatctg gctgttgaat gattaccgtg gcgttcccac tcctgttctg ctgctgacgt
    12361 tgctgttact cggcggaatg tttatggcaa cgcggacggc atttggacga cgcatttatg
    12421 ccatcggcgg caatctggaa gcagcacgtc tctccgggat taacgttgaa cgcaccaaac
    12481 ttgccgtgtt cgcgattaac ggattaatgg tagccatcgc cggattaatc cttagttctc
    12541 gacttggcgc tggttcacct tctgcgggaa atatcgccga actggacgca attgcagcat
    12601 gcgtgattgg cggcaccagc ctggctggcg gtgtgggaag cgttgccgga gcagtaatgg
    12661 gggcatttat catggcttca ctggataacg gcatgagtat gatggatgta ccgaccttct
    12721 ggcagtatat cgttaaaggt gcgattctgt tgctggcagt atggatggac tccgcaacca
    12781 aacgccgttc ttgattttga taaaaatttt ctcaaagccg gttacgtatt accggttttg
    12841 a
//
LOCUS       DADLSB010000109        12802 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.109, whole genome shotgun sequence.
ACCESSION   DADLSB010000109 DADLSB010000000
VERSION     DADLSB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.109"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(18..377)
                     /gene="frdD"
                     /locus_tag="JN331_002931"
     CDS             complement(18..377)
                     /gene="frdD"
                     /locus_tag="JN331_002931"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY7261028.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(388..783)
                     /gene="frdC"
                     /locus_tag="JN331_002932"
     CDS             complement(388..783)
                     /gene="frdC"
                     /locus_tag="JN331_002932"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY7261029.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(794..1528)
                     /locus_tag="JN331_002933"
     CDS             complement(794..1528)
                     /locus_tag="JN331_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY7261030.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(1521..3329)
                     /gene="frdA"
                     /locus_tag="JN331_002934"
     CDS             complement(1521..3329)
                     /gene="frdA"
                     /locus_tag="JN331_002934"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY7261031.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLD
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3654..4631
                     /gene="epmA"
                     /locus_tag="JN331_002935"
     CDS             3654..4631
                     /gene="epmA"
                     /locus_tag="JN331_002935"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY7261032.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            4850..6352
                     /gene="yjeM"
                     /locus_tag="JN331_002936"
     CDS             4850..6352
                     /gene="yjeM"
                     /locus_tag="JN331_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY7261033.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMLSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(6503..9814)
                     /gene="mscM"
                     /locus_tag="JN331_002937"
     CDS             complement(6503..9814)
                     /gene="mscM"
                     /locus_tag="JN331_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY7261034.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPML
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYFLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICCALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(9836..10804)
                     /gene="psd"
                     /locus_tag="JN331_002938"
     CDS             complement(9836..10804)
                     /gene="psd"
                     /locus_tag="JN331_002938"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY7261035.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLACNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(10901..11953)
                     /gene="rsgA"
                     /locus_tag="JN331_002939"
     CDS             complement(10901..11953)
                     /gene="rsgA"
                     /locus_tag="JN331_002939"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY7261036.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            12048..12593
                     /gene="orn"
                     /locus_tag="JN331_002940"
     CDS             12048..12593
                     /gene="orn"
                     /locus_tag="JN331_002940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY7261037.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         2910 a         3331 c         3398 g         3163 t
ORIGIN      
        1 tttacattgg cgatgcgtta gattgtaacg acaccaatca gcgtgacaac tgtcaggata
       61 gcagccagac cgtagaaaac ccatttgccc gcaggcacgt ggattttcag atcgtgcatc
      121 gcgtggtgca tacggtgtaa accacaccac agcggcagaa cgatcatcag gaacaggaat
      181 acgcgaccaa tgaagctctg cgcgaacgcc agaacgcgct cgtagctcag cgcatcaccc
      241 ggaaacagcc ccagtggcag cagaataccc accagcagga tcatcaccgg cgcaataatt
      301 gcgctccaca taccaccggc cccgaagagg ccccagaata ccggttcgtc agaacgcttt
      361 ggatttggat taatcatctc aggctcctta ccagtacagg gcaacaaaca ggattacgat
      421 ggtggcaacc acagttaccg cccagagact tttgataatt ggctctggtc ccattttttc
      481 gtcttttaca atgatattgg ccgctttcgg tgccagttca aaccaggttt tggtgtgcag
      541 cagagctgcc gtcagagtga tcaggttaat gatcacgata accgggtttt gtaaaaagtc
      601 gacgaatccc gcccaggctt ccgggccatt tttcagggca aacagcccga aaatcagttc
      661 aatgctgaac cacacagccg gaaccgccgt gccttcgcgc agcatgtaaa agcgataaaa
      721 cggcaatttt ttccaccagg tggacgtcat tggccgtaca tacggtttac gtttagtcgt
      781 catgttgcac tccttagcgt ggtttcaggg tcgcgataag aaagtctttc gaactttcta
      841 ctttgccctg ctgaatggcc gcagccggat cgacgtgttt cgggcagact tcggagcaat
      901 agcccacgaa agtacagctc catacaccgt tctggctgtt caactgcgcc atacgctcct
      961 tcttaccgtg gtcgcggcta tcttcgttat aacgatgcgc cagcgtaatg gcagccggac
     1021 cgatgaactc tgggttcagg ccaaactgcg ggcacgcggc gtagcacaga ccacagttga
     1081 tgcaaccgga gaactggtga tactttgcca tctgcgccgg ggtctggatg ttagtaccct
     1141 gatccgcggt gcgggagttg ccgatgatgt acggtttgat cgcttccaga ctttcgatga
     1201 agtgggtcat atcgaccacc agatcgcgtt caatcgggaa gttagctaac gcttcaacct
     1261 tcataccgtc ggtgtaatca cgcaggaagg ttttacatgc cagttttggc acgttgttaa
     1321 ccatcatgcc gcaggaacca caaatcgcca tacggcagga ccagcggtaa ctcaggtccg
     1381 gtgccaggtt gtctttgatg tagcccagcg catccagtaa tgaggtagtt gcgtcataag
     1441 gcacttcata gaatgcgcta tgcggtgcgg tatcgacttc cgggttatag cgcaccacct
     1501 caattttcag gtttttcatc tcagccattc gccttctcct tcttattggc tgcttccgcc
     1561 ttatcggctg catccgcttc gccaccgtaa acgcgtttag ctggcggcag cgtagtaatc
     1621 ttcacgtcgc tgtactccag gcgagtcgtg ccatcagcat cgcggaaggc gagggtgtgt
     1681 ttgagaaagt tgacgtcgtc acgctcggtg caaccttcgt ccagacgctg gtgtgcgccg
     1741 cgggactctt tacgtgccat tgcggagtgc gccatacatt cagcaacgtt cagaccgtgg
     1801 cccagttcaa tggtgtagag caggtcggtg ttgaacacgc tggaggtgtc ggtgatgcgc
     1861 acgcgcttga agcgttcctg cagctctgcc agcttgtcga tggttttctg catcagttcc
     1921 ggcgtacggt agataccgca accttcttcc attgccaggc ccatttcgtc gcggatcttc
     1981 gcccagtttt cgccgccatc ctggttaacc agatctttca gacgttgttc aacgccagct
     2041 gcctgcgctt caattgccgc ttcgttgcca ttaccggcag ttgctgcacg ctctgtcgct
     2101 tgttcaccgg ccagacggcc gaagaccacc agttccgcca gggagttgga gcccagacgg
     2161 tttgcaccgt gcagaccaac agaggaacat tcacccacgg cgaacagacc tttaatgcgg
     2221 gtttcacagt tctgatcggt ttcgataccg cccatggtgt agtgtgcagt cggacgtacc
     2281 ggaatcggtt ctttaaccgg atcgacgcca acgtacgctt tcgccagttc gcagatgaac
     2341 ggcagacgtt catgcagttt tttctcgccg aggtgacgca ggtcgagata aaccacatca
     2401 ccacgcggcg tggagatggt gttgccttta cgccattcgt gccagaaggc ctgagaaact
     2461 ttgtcgcgtg gacccagttc catatatttg tttttcggct cgtccagcgg agtttccggg
     2521 cccatgccgt aatcttgcag ataacggtag ccatttttgt tgaccagaat accgccttca
     2581 ccacggcagc cttcggtcat caggataccg gaacctggca gaccggttgg gtgatactga
     2641 acgaattcca tgtcacgcag cggaacgccg tggcttagcg ccatacccat accgtcaccg
     2701 gtaacgatgc cgccgttggt gttgtaacga taaacgcgac ccgcaccgcc ggtagccata
     2761 acgaccgcgt tagcacggat ctgcaccaac gtgccttcca tcatgttcat tgctaccagg
     2821 ccgcgaacat gaccatcatc aaccagaata tccagcacga aatgttcgtc aaaacgctgg
     2881 atctgcggga attgcagaga ggtctggaac agcgtgtgca gcatatggaa gccggtctta
     2941 tcggcggcga accaggtgcg ctcgattttc atgccgccga agcgacgtac gttgacgcta
     3001 ccatccgggc gacggctcca tgggcatccc cacagttcca gttgggtcat ttcggttggg
     3061 cagtggtgga cgaaataatc cacgacatcc tgctcacaca accagtcgcc acccgctact
     3121 gtatcgtgaa agtgatattc gaagctgtca tgatcctgcg cgacagcggc ggagccccct
     3181 tctgcagcaa cggtatggct acgcatcggg tatacttttg agattagtgc gatttttgca
     3241 tttggatttg cctgcgcggc agcaattgca gcacgtaatc ccgcgccacc ggcgcctaca
     3301 atggcaagat cggcttgaaa ggtttgcacg acattcctcc agattgtttt tatcccacag
     3361 ccccgtactt cagggtaagt gcctgaaagt tacggtctgc gaacgctatt ccactgctcc
     3421 tttataggta caacagtata gtctgatgga taagtctgaa atttgacgag atcgattttt
     3481 ttagtgcgca agagggctaa attatcactg aagatgatta atttaattac taaaccatca
     3541 gatcgtgctt tttttagtca ctgcccaccg ctgtttgatt cctgcgtaaa atttgtctcg
     3601 ccgtcgcgtt gcgagtagac ttcgtgccct tgtcaaaaac tggagattta actatgagcg
     3661 aaacggcatc ctggcagccg agcgcatcca ttcctaactt attaaaacgc gcggcgatta
     3721 tggcggagat ccgtcgtttc tttgccgatc gtggagtgct ggaggtggag acgccttgta
     3781 tgagccaggc gacggtaacc gatattcatt tggtcccgtt tgagacacgt ttcgttggcc
     3841 ccgggcattc gcaggggatg aatctctggt taatgaccag cccggaatac catatgaaac
     3901 gcctgctggt tgctggttgt gggccggtat tccagctgtg ccgcagcttc cgtaatgaag
     3961 agatggggcg ttatcacaac cctgagttca ctatgctgga gtggtatcga ccgcactatg
     4021 atatgtaccg gttgatgaac gaggtggacg atctcttaca acaggtgctg gactgcccgg
     4081 cagcagaaag cctttcttat caacaagctt tcttgcgtta tctggaaatt gacccgctct
     4141 ctgccgacaa aacgcaactg cgggaagtcg cagcgaaact ggatttgagc aatgttgctg
     4201 ataccgaaga agaccgcgac acgttgctac aattgctgtt tacctttggc gtagagccaa
     4261 atattggtaa agaaaaaccg acctttgtgt accactttcc agccagccag gcatcactgg
     4321 cgcaaatcag taccgaagat catcgggtcg ctgaacgctt tgaggtttat tataaaggta
     4381 ttgagctggc gaatggtttc catgaattaa cggatgcccg tgagcagcaa caacgctttg
     4441 aacaagataa ccgtaagcgc gcggcgcgcg gtttgccgca gcaccccatt gaccagaatc
     4501 tgattgaagc cttgaaagtc ggtatgcctg actgttccgg cgtggcatta ggtgttgatc
     4561 gtctggtgat gttggcgctg ggcgcggaga cactggctga agtcatcgcc tttagcgttg
     4621 accgggcata attctgaaaa attactgaat taacagcgaa gaatggcgtg ataagcgcca
     4681 ttctgtagca tatgttttca tttttccgtt aaaggccaca atttaagact ggatatttga
     4741 tatcatccag gtatcaattc tgtattggtt tttacgctgc ctgctcaatg ttgcgcagtg
     4801 cagttcagtg ggcacgtgtt atacacgcgc tgaaatgaag gatggtttca tgcctcacac
     4861 gataaaaaag atgagtctga taggactcat attgatgatc tttacttccg tatttggatt
     4921 tgccaatagc ccatcggctt attacttaat gggttatagt gcgattccct tttatatatt
     4981 ttctgcattg ttattcttta ttccattcgc cttaatgatg gctgaaatgg gagctgctta
     5041 tcgcaaagaa gaaggtggta tctattcttg gatgaataat agtgtcggac cacgttttgc
     5101 cttcattggt acgtttatgt ggttttcctc ttatatcatc tggatggtga gtacctccgc
     5161 gaaagtttgg gtaccgttct caacattcct ctatggtagt gacatgaccc agcactggcg
     5221 tattgccgga ctggagccta cgcaggtggt tggtctgctg gcagtagcat ggatgattct
     5281 ggtcaccgtc gttgcttcaa aggggattaa taaaattgcc cgcattactg cggtgggcgg
     5341 tattgcagta atgtgtctga atttagtatt gctgttagta agcattacta ttttgttatt
     5401 aaatggtggg catttcgcgc aggatattaa tttccttgca tcaccgaacc cgggttatca
     5461 gtccggtctg gcaatgctat cgtttgtggt atttgctatt tttgcctatg gcggaattga
     5521 agcggttggt ggtctggtcg ataaaacgga aaatccagaa aagaactttg ccaaaggtat
     5581 tgtttttgcc gctattgtta tttcaatcgg ttattcgctg gcaatatttt tatggggcgt
     5641 cagcacaaac tggcagcagg tattaagtaa tggttccgtt aaccttggca atattaccta
     5701 tgtgctgatg aagagcctcg gggtgacgct gggtaacgca ctgcatttgt cacctgaagc
     5761 gtcattgtcg ctgggcgtat ggtttgcgcg tattaccgga ctttcgatgt tcctcgctta
     5821 taccggtgcg ttctttacgc tttgctattc accgctgaaa gccatcatcc aggggacgcc
     5881 gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg atggggatgc tgtctatcgc
     5941 catgtggatg cagtgcgggt tggttactgt cttcattctg ctggtttcgt ttggtggcgg
     6001 taccgcatcg gcgttcttta acaagctgac gctgatggcg aacgtgtcta tgacgcttcc
     6061 ttatctgttc ctcgcgctgg ctttcccgtt ttttaaagca cgtcaggatc tcgacagacc
     6121 atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg acggtggttg tcgttctggt
     6181 ggtgacattt gcgaacgtct tcaccattat tcagcctgtg gttgaagccg gagactggga
     6241 cagcacattg tggatgattg gcggccctgt cttcttctcg ctgttagcga tggcgattta
     6301 ccagaactat tgcagtcgca tggcgaataa acctgagtta gctctcgact gatcatatga
     6361 aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag gaagtaatgc gtatggatga
     6421 tactctccag ggccggtgat aactgaagat gatgttaagc tgctaaatga gccacgttga
     6481 catcatttgg cagcttcatg cattacaaac ttcccgcctg acgacctttg cccgcagacg
     6541 tcagcgttct ccccgtttgt ttaccgttga ggctttccag acgcatctgg aagggcggga
     6601 atggcatatc gataccgtgg gcgtggaagc ctgccagaat cagctggtgg atctcatggc
     6661 gtagcggcat acggtgaccc atctcagcgg cgtaaatacg cagctcgaaa atctgaatcc
     6721 cctgttgcag atccaccagg aagacttccg gtgccgggtt gtcgatcacc agcgagcagc
     6781 gacgcgctgc ggtgagcagg atttccgtca cttcttcact gttggcatcg gcaggggccg
     6841 gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga ccagttgata aactgctcgg
     6901 taataaacgc cttgttcggc acgattatct ctttacggtc ccagtcgctg atggtggtgg
     6961 cgcgggtgtt aatttttgtc acgctaccag taagatcgcg gatcgttacc gtatcgccaa
     7021 tgcgaatcgg tttttcgaac aggatgatca ggccagagat aaagttggcg aaaatttcct
     7081 gcaaaccaaa accgagacca acaccgagcg cggcaaccag ccactgcaat ttcgaccact
     7141 caataccaat cattgagaag ccgaccagcc cgccaatcag catcagcaga tattttgtga
     7201 tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag gtgctgcaaa atcgccagtt
     7261 ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat gataaatacc agaatggcaa
     7321 tcagcaccgc accgagggta attggctcca gactttctac gccctgtacc gtggaggtga
     7381 catcccacag cgaaatattt tcgaggaagc cgaaagcgga atggatttct gaccacagca
     7441 caatgaccga aagcagggcg atcaacatca aaattgagcg caccagccgc aaggattgcg
     7501 cactgatggc atcgagatcg acttcgcttt catcgacttc aattgctcct tccgggctac
     7561 tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc taacatctct gcccggcgat
     7621 gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag catccagcgg cggataacgt
     7681 gataaaccac cagtagcagg aaccagatgg caaccgaggt ttcaagcctc gctaacagtg
     7741 cctgcgccgt tgccagataa cccaccgccg acgccagaat ggcaaccagt ggtgcgccaa
     7801 tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc gctgccttct ttgttgagat
     7861 acaacggaat accggctttt ttcaggctta gggtgaccac cgccagcgca cagcaaatga
     7921 ggataaagca aagccgtccc agcgaaccgg aaaattcgcg gtcgtcgagg ttatcgaaca
     7981 tcatcagcgc cataatcagc ccgatgctca tcaggaagta gcgcatccca cgggaaacac
     8041 gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt cgggcgggca aaggtggcgc
     8101 aaatcatcac tacccacagc atcggcacgg tggccgttac accatcgcca atcgcgaccg
     8161 ccagcggata aggccacgcc tcgcgcaagc cgtaacccag cgtcatccac agcaccggta
     8221 acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa cgtcagccag aagtgatcct
     8281 gagtcacttt gccgactttc gccgccgaac gttcaagaaa acgggtgaaa tagcggcgcg
     8341 agtaaatact gcaaccgacc agaatcaacg cgccaaacag cggcaaaatt gtctctttgc
     8401 tggtcagcat catcacactg gctttgccca actgactgaa ggtgtccagc gaaatgagac
     8461 gacgcagatc ctgggcgatt tccagcggcc aggcgatggt catcgggcgc acgtcagagg
     8521 tccagaacag atagcggtgc gttgcttcgt tcacctcttt cagcgcatcc tccagttgcc
     8581 cgttggagac tttcagcttg gtcagttcca atagtagcgt gtcgccaccc tgcaacaatg
     8641 agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag aatacggttt tgctcggcgg
     8701 tcagcggctg accgtcggcc tgatggattt gccgtagcag cggctgttta ttaagtaaat
     8761 cctcataacg taaccgttgc acacgcaact gcgccatttc ggtatcaagc tgttgtggtt
     8821 ttggcatttc tggtagccgt gccacctgcg cccgcagcgc ttcgccgagc aggttggacg
     8881 atcccagcca ttgcgactgt tcacgcagcg tattcaacgc ctgccggacc tgtaacgtct
     8941 ggctggcagc ctgacgctgt tgcgaggcaa cgagatccat ccgctgcgcc tgttgattca
     9001 gcgctgccga tagttcgcgg ttaattttga attgcgcgac gatatctttc ggcaaatcgg
     9061 cactgttttc tgccagtaat tcggtacttt ccagcgcccg ctccgcctca agctgacgtt
     9121 ggctattcaa ctgattacgt aaggcctgca aatacgcatc cagttgctgg ctctcttttt
     9181 ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt attggcagac agctgcgcca
     9241 gctccagttc atcaacgagc gccttaagac gtgcagagtc agactgcaac gcgaaatttt
     9301 gtgcctgatt gagcggagta ttgccggtaa gcgttcccag gcggcgctcg atctcattta
     9361 actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt cagcgaatcg gcaatctcgc
     9421 gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc cagcaactgg ctgctgacct
     9481 ggagaatttc ctgattcagc gcgtcggtag acattcccgg cgacacgctg cgcggctcgt
     9541 cacgcatgtt gtttaattgt gcgcgcagag tagcggagag tttcggataa ttatcgataa
     9601 cttgctgata ttgtttgatg cgctcaaggg aaccttttcg ttcctcaagc gcatttaagg
     9661 cagactggag cgcctctacg acttccggct gtgcgggttt cgccgctttt gcctgctcca
     9721 gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc gtacgccccc caactgaggc
     9781 accaggccat cagaaaagtg ataatcaggc gcacgtcagc gtttcctttg atggattaga
     9841 cctggtcttt tttatcgtca accaatgggc tggcgtcgtg ttctgcttcg atctcttcag
     9901 caggaagtgg ggcgggttca gcgtctggcg taacaaaggt ttcggtagat actgccagcg
     9961 gctggccaat tttcgtgaca gacaggcttt ccagttgctc aaccagattc actttacccg
    10021 gtgcaaacag gttgataacg gtggaaccga gtttaaagcg acccatttcc tggcctttca
    10081 gcagtgccac agaaccgtcg ttttccccgg caggccaggt ccagcgcttg atgatacctt
    10141 cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat gctgccaaca atcgtcgctc
    10201 cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa aaggcaaatc acgcgttcgt
    10261 tacgggcaaa cagattcggc acgttctgag cggtgagatg gttaacggaa aagagatcgc
    10321 ccggcacgta gatcatctca cgcagaatac cgttgcacgg catgtgtacg cgatggtagt
    10381 cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg gaacaggtcc gccatcagat
    10441 agttgcatgc cagcagggct tcgaggctgt agttgtggcc tttggcttgc aggattttat
    10501 cttcttcgat tttacccaac tggctgataa cgccatcggc aggcatgacc agtacattcg
    10561 gatcggtatc gattgggcgt acttcgtcac gcagcggacg gacaaagaat tcgttaaagg
    10621 tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat gtcgaccttg tagtatttaa
    10681 cgaacagatc gataaccagt tttgtcagcc atcctgcccg cttgcttgcg ccccaacccg
    10741 ccaggcgagt aagccatagt ttcggcagaa tgtactgtag cgaaagttta aatgaattta
    10801 acaaggtagc ctccaggcca ttgttttgtc gttcctgatc cggccaacat gccggatcct
    10861 gaaaaaaagg ggacgattct aacgacggtt agcttaattg tcagtcatcc gtatcagaaa
    10921 agtttttacg cgtttttacc tgcgccatgc tttccagaat acggtgatag ttttcgaaac
    10981 gggtttccgc gattttccct tcctcaaccg cttcccggat agcgcagccc ggatcggtat
    11041 cgtgtttgca atcgcgatat ttacacagac ctaaatagtc atggaattcg acaaagccct
    11101 gagtgatttg ttccggctcc aggtgccaga ggccgaactc acgcacacca ggggaatcaa
    11161 tcacatcacc gccgtgcggg aagtgataca gccgagcggc ggtcgtggtg tgctgaccga
    11221 gccccgagtt gtcagagaca tcgttggtca ggatctcttt ttgcagcccc agcaacgcgt
    11281 tcagcaggct ggatttaccg acgccagact gcccggcaaa aatgctgatg cgcccggtca
    11341 acgcctcttc cagcggtttc agcccatcct gagtatggct ggaaaccatc aatacgcgat
    11401 aaccgatgtt gcggtagata tccatctgct cgttgacgaa cgccatgcct tcgtcgtcca
    11461 gcaggtctat cttgttgagc acgataatcg gctcaatctg caaggtttcg caggccacca
    11521 ggtaacggtc gataatattg agcgacagct ccggcaaaat ggcggagaca atgacaatct
    11581 ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa atccggacgc gttaacaccg
    11641 aggtgcgctc atgcaccgct tccacgatcc ctttgacatt tacgccttcc gccgccggtt
    11701 tacccggacg ccagactacg cggtcgccgg ttaccagcga acggatggta cggcgaatat
    11761 tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc gtgcatacca aagcggctga
    11821 tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc gtagtcgggc ttctccttag
    11881 acgttttaag acgacgctgg tgattggcgt tcacgcggcg ctgctggcct ttggagagtt
    11941 tatttttact caatcgtact gactcctggt cgcccctgat gggcaaaaca tctatgatac
    12001 acgcaattgt ggatcaatat agtcactgtg aatgggtgga aaatagcatg agtgccaatg
    12061 aaaacaacct gatttggatc gatcttgaga tgaccggtct ggatcccgag cgcgatcgca
    12121 ttattgagat tgccacgctg gtgaccgatg ccaatctgaa tattctggca gaagggccga
    12181 ccattgcagt acatcagtcc gatgaacagc tggcgctgat ggatgactgg aacgtgcgca
    12241 cccataccgc cagcgggctg gtagagcgcg tgaaagcgag cacgatgggc gatcgggaag
    12301 ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc tgcgggaaaa tcgccgattt
    12361 gcggtaacag catcggacag gaccgtcgtt tcctgtttaa atacatgccg gagctggaag
    12421 tctacttcca ctaccgttat ctcgatgtca gcaccctgaa agagctggcg cgccgctgga
    12481 agccggaaat tctggatggt tttaccaagc aggggacgca tcaggcgatg gatgatatcc
    12541 gtgaatcggt ggcggagctg gcttactacc gcgagcattt tatcaagctg taaaattttt
    12601 cctggtcacg taagcgccgg aatccacttg cggcggcgtg attttgacgc taaatcgaac
    12661 gtcttgctga taatttcagc acttgagata aaaacgcaaa aaaaactttt ttggggggtt
    12721 gcagagggaa agatttctcg tataatacgc ctcccgtaac gacgcagaaa tgcgaaaatt
    12781 acgaaagcaa aattaagtag ta
//
LOCUS       DADLSB010000110        12529 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.110, whole genome shotgun sequence.
ACCESSION   DADLSB010000110 DADLSB010000000
VERSION     DADLSB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.110"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..379
                     /locus_tag="JN331_002941"
     CDS             <1..379
                     /locus_tag="JN331_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7261038.1"
                     /translation="QRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAP
                     TADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTP
                     RYDKSAADLAWQRTLKWFDKYLS"
     gene            498..785
                     /locus_tag="JN331_002942"
     CDS             498..785
                     /locus_tag="JN331_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY7261039.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            974..2092
                     /gene="hybO"
                     /locus_tag="JN331_002943"
     CDS             974..2092
                     /gene="hybO"
                     /locus_tag="JN331_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY7261040.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            2095..3081
                     /gene="hybA"
                     /locus_tag="JN331_002944"
     CDS             2095..3081
                     /gene="hybA"
                     /locus_tag="JN331_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY7261041.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            3071..4249
                     /gene="hybB"
                     /locus_tag="JN331_002945"
     CDS             3071..4249
                     /gene="hybB"
                     /locus_tag="JN331_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY7261042.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            4246..5949
                     /gene="hybC"
                     /locus_tag="JN331_002946"
     CDS             4246..5949
                     /gene="hybC"
                     /locus_tag="JN331_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY7261043.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            5949..6443
                     /locus_tag="JN331_002947"
     CDS             5949..6443
                     /locus_tag="JN331_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY7261044.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            6436..6924
                     /gene="hybE"
                     /locus_tag="JN331_002948"
     CDS             6436..6924
                     /gene="hybE"
                     /locus_tag="JN331_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY7261045.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMYDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            6917..7258
                     /gene="hypA"
                     /locus_tag="JN331_002949"
     CDS             6917..7258
                     /gene="hypA"
                     /locus_tag="JN331_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7261046.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCRGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            7392..7640
                     /gene="hybG"
                     /locus_tag="JN331_002950"
     CDS             7392..7640
                     /gene="hybG"
                     /locus_tag="JN331_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY7261047.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(7763..8629)
                     /gene="yghU"
                     /locus_tag="JN331_002951"
     CDS             complement(7763..8629)
                     /gene="yghU"
                     /locus_tag="JN331_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY7261048.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            8834..10693
                     /gene="gss"
                     /locus_tag="JN331_002952"
     CDS             8834..10693
                     /gene="gss"
                     /locus_tag="JN331_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY7261049.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            10985..12484
                     /gene="pitB"
                     /locus_tag="JN331_002953"
     CDS             10985..12484
                     /gene="pitB"
                     /locus_tag="JN331_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY7261050.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRSFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT         2899 a         3109 c         3449 g         3072 t
ORIGIN      
        1 gcaacgctat ccgcaagcga caggtaaagt gggtattacc ggattttgct atggcggtgg
       61 cgtatcgaac gcggcggctg tcgcgtatcc ggaactggcc tgcgcggtgc cgttttatgg
      121 tcgtcaggca cccactgccg atgtggcgaa gattgaagca cctttactac tccacttcgc
      181 ggaactggac acccgaatca acgagggctg gcctccttac gaggcggcgt tgaaagacaa
      241 taataaggtt tatgaggctt atatctatcc gggggttaat cacggattcc ataatgattc
      301 cacgccccgt tatgacaaat ctgccgccga tcttgcctgg caaaggacac tgaaatggtt
      361 cgataaatat ctctcctgat aggtttatct cttacgggat tacgtcttaa acaagtatga
      421 aaaaatagcg tgcgcaaaag tcgttctttg cctaaaatat cgttatatat ggcgatatat
      481 aacgaatgag gtgaacgatg aataaccatt ttggtaaagg cttaatggcg ggattaaaag
      541 caacgcatgc cgacagtgcg gttaatgtga caaaattctg tgccgattat aaacgcggtt
      601 ttgtattagg ctactcacac cggatgtacg aaaagaccgg agatcgccag cttagcgcct
      661 gggaagcggg tattctgacg cgccgctatg gactggataa agagatggta atggatttct
      721 ttcgtgagaa taattcctgt tctacgttgc gcttttttat ggccggttat cgcctcgaaa
      781 attgatcaaa catacgtatt atcttgcttt aattaattac actaatgctt cttcccttcg
      841 ttttagcgcc ccgccgcagt atcatgatat cgataaccat aataaatgtg tggtaaatgg
      901 cgcatcgatc gcattattga ttttgcgatt gaggcaaaat atatgccagg tcttcgcaac
      961 ggaataacta taaatgactg gagataacac cctcatccat tctcacggca ttaaccgtcg
     1021 tgatttcatg aagctttgtg cagcattagc cgccaccatg gggttaagta gcaaagccgc
     1081 tgcagagatg gccgaatcgg ttactaaccc gcagcgtccg ccagttatct ggattggcgc
     1141 gcaggagtgc accggttgta cggaatctct gcttcgtgca acgcatccaa cggtagaaaa
     1201 cctcgtgctg gagactatct ctctggaata tcacgaagtg ctttccgccg ccttcggtca
     1261 tcaggtcgaa gagaacaaac ataacgctct cgagaagtac aaagggcagt atgtgttagt
     1321 ggtggatggt tccatcccat taaaagataa cggtatttat tgcatggttg ccggtgagcc
     1381 gattgtggat cacatccgca aagcggcgga aggcgcagca gccattatcg ctattggttc
     1441 ctgctccgcg tggggcggtg ttgccgcagc tggagttaac ccaactggcg cagtcagctt
     1501 gcaagaagtt ctgccaggca aaaccgttat caatattccg ggctgcccgc cgaacccgca
     1561 caacttcctc gcgaccgttg cgcacatcat cacttacggc aaaccgccga aactggatga
     1621 caaaaaccgt ccgaccttcg cctatggccg tctgattcac gaacactgcg aacgtcgccc
     1681 gcacttcgat gctggtcgtt ttgccaaaga gttcggtgat gaaggccacc gcgaaggctg
     1741 gtgcctgtac cacctcggct gtaaagggcc agaaacttac ggcaactgct caacgctgca
     1801 attctgcgat gttggcggtg tgtggccggt ggcgattggt cacccgtgct atggctgtaa
     1861 cgaaaaaggt atcggcttcc ataaaggcat ccatcagctt gccaacgtcg aaaatcagac
     1921 tccgcgttca cagaaaccgg atgttaacgc taaagagggc ggcaacgtct ctgcaggcgc
     1981 tattggtttg ctcggcggtg tggttgggct ggttgccggt gtcagcgtga tggcggtgcg
     2041 tgaactgggt cgtcagcaaa agaaagataa cgctgactca cggggagaat aaccgtgaac
     2101 agacgtaatt ttattaaagc agcctcctgc ggggcattgc tgacgggcgc gctgccgtct
     2161 gtcagtcatg cggctgctga aaaccgcccg ccaattccgg gatcgctggg gatgttgtac
     2221 gactcgacct tgtgcgtagg ctgccaggct tgcgtcacca agtgtcagga tatcaacttc
     2281 cctgaacgta acccgcaagg ggaacagacc tggtcgaaca acgacaaact gtcgccgtat
     2341 accaataata tcattcaggt gtggaccagc ggcacagggg tcaacaaaga ccaggaggag
     2401 aacggctacg cgtacattaa gaaacagtgt atgcactgcg tcgatccgaa ctgtgtctct
     2461 gtgtgcccgg tctctgcact gaaaaaagat ccgaaaaccg gcattgtcca ttacgacaaa
     2521 gacgtgtgca ccggttgccg ttactgcatg gtcgcctgtc cgtacaacgt gccgaagtac
     2581 gactacaaca acccgtttgg tgcgctgcat aagtgcgagc tgtgcaacca gaaaggtgtg
     2641 gaacgtctcg ataaaggcgg tctgcctggc tgcgtagaag tgtgcccggc gggcgcggtg
     2701 attttcggta cgcgtgaaga gctgatggcg gaggcgaaaa aacgtctggc gctgaagcct
     2761 ggcagcgaat accactatcc gcgtcagacg ctgaaatctg gcgacactta cctgcatacg
     2821 gtgccgaaat attatccgca tctgtacggc gagaaagagg gcggcggtac tcaggttctg
     2881 gtactgacgg gtgtgcctta tgaaaatctc gacctgccga aactggacga tctttctacc
     2941 ggtgcgcgtt ccgaaaatat tcaacacacc ctgtataaag gcatgatgct accactggct
     3001 gtgctggcgg gcttaaccgt gctggttcgt cgcaacacca aaaacgacca tcacgacgga
     3061 ggagacgatc atgagtcatg atccacaacc gctgggcggc aaaatcatca gtaaaccggt
     3121 catgattttt ggaccgttaa tcgtcatctg tatgctcctg attgtgaagc gtctggtgtt
     3181 cggtctgggc tctgtctctg acctgaacgg cggcttcccg tggggcgtgt ggatcgcgtt
     3241 tgacctgctg attggcaccg gctttgcctg tggcggctgg gcgctggcgt gggcggtata
     3301 cgtctttaac cgtgggcaat accatccgct ggtgcgtccg gcgctgttgg cgagtctgtt
     3361 tggttactca ctgggtggct tgtcgatcac tatcgacgtg ggtcgctact ggaacctgcc
     3421 gtacttctac attccgggtc acttcaacgt gaactcggta ctgttcgaga cggcggtctg
     3481 tatgaccatc tacatcggcg tgatggctct ggagtttgct ccggcactgt ttgaacgtct
     3541 gggctggaag gtgtcgctac aacgtctgaa caaggtgatg ttcttcatta tcgcgctcgg
     3601 tgcgctgctg ccgaccatgc accagtcttc aatggggtcg ctgatgatct cggcgggcta
     3661 caaggtgcat ccgctctggc agagctatga aatgttgccg ctgttctcgc tgctgacggc
     3721 gttcatcatg ggcttctcga ttgtcatctt tgaaggttca ctggtgcagg cgggtctgcg
     3781 tggcaacggt ccggatgaaa agagcctgtt cgtcaagctg accaacacca tcagtgtgtt
     3841 gctggcgatt ttcatcgggc tgcgctttgg cgagcttatc tatcgcgaca agctgtcgtt
     3901 agcgtttgcc ggtgacttct actccgtgat gttctggatt gaagtcctgc tgatgctctt
     3961 cccgctggtc gttctgcgtg tggcgaagct gcgtaatgat tcccgcatgc tgttcctgtc
     4021 agcactgagc gcactgttag gttgtgcaac ctggcgtctg acctattcgt tggtggcatt
     4081 caacccgggc ggcggttacg cctacttccc aacctgggaa gaactgttga tttctattgg
     4141 ttttgtggct attgagattt gcgcttacat cgtactcatt cgtctactgc cgatacttcc
     4201 tcctttaaaa caaaacgatc ataatcgtca tgaggcgagc aaagcatgag ccagagaatt
     4261 actattgatc cggtaacccg tattgagggg catttacgca tcgattgcga aatcgaaaat
     4321 ggcgtcgttt cgaaagcatg ggcttccggt accatgtggc gcggcatgga agagatcgtg
     4381 aaaaaccgcg atccgcgcga tgcatggatg attgtgcaac gtatctgtgg cgtatgtact
     4441 accactcacg cgctgtcttc cgttcgtgcg gcagaaagtg cgctgaatat cgacgttccg
     4501 gttaacgcgc aatacatccg taacatcatt ctggctgcgc acaccacgca tgaccatatc
     4561 gttcatttct atcagctttc ggcgctggac tgggtggaca tcacttctgc actgcaagct
     4621 gacccaacca aagcctccga aatgctgaaa ggcgtttcga cctggcatct gaacagtccg
     4681 gaagagttca ctaaagttca gaacaagatc aaagatctgg ttgctagcgg tcagttgggt
     4741 attttcgcca atggctactg gggtcacccg gcgatgaaac tgccgccgga agtgaacctg
     4801 attgcggtag cgcactacct gcaggcgctg gagtgccagc gtgacgctaa ccgcgtcgtg
     4861 gcgctgctgg gcggtaaaac gccgcacatt cagaacctgg cagtgggtgg tgtcgcgaac
     4921 ccaatcaacc tcgacgggtt gggcgtgttg aaccttgagc gcctgatgta catcaagtct
     4981 ttcatcgaca aactgagcga ctttgttgag caggtttata aggttgatac cgcggttatc
     5041 gccgcgttct acccggaatg gctgacgcgc ggtaaaggtg cggtgaacta cctgagcgtg
     5101 ccggaattcc cgaccgacag caaaaacggc agcttcctgt tcccgggcgg ctacattgag
     5161 aatgcggatc tgtcctcgta tcgtccgatc acttctcatt ccgatgaata cctgatcaaa
     5221 ggcattcagg aaagcgcgaa gcactcctgg tataaagacg aagcgccaca ggcgccgtgg
     5281 gaaggtacca ccattccggc ttatgatggt tggtctgacg acgggaaata ttcctgggtg
     5341 aaatcaccga ctttctacgg caaaacggta gaagtgggtc cgctggctaa tatgctggtg
     5401 aaactggcgg caggtcgcga atctacccag aacaaactga atgaaatcgt tgcgatttat
     5461 cagaaactga ctggcaacac gctggaagtg gcacagctgc actccacgct gggccgtatt
     5521 attggtcgta ccgttcactg ctgtgaattg caggatatcc tgcaaaacca atacagtgca
     5581 ctgatcacca atatcggcaa aggcgatcac accacctttg tgaagccgaa cattccggca
     5641 acgggtgagt tcaaaggtgt tggcttcctc gaagcgccgc gcggtatgct ctctcactgg
     5701 atggttatta aagacggtat catcagcaac taccaggcgg ttgttccatc aacctggaac
     5761 tctggtccgc gtaacttcaa tgatgacgtc ggtccttacg agcagtcgct ggtgggtaca
     5821 ccggttgccg atccgaataa accgctggaa gtggtgcgta ccattcactc cttcgacccg
     5881 tgcatggcct gtgcggtaca cgtagtggat gccgacggca acgaagtggt ttcagtgaag
     5941 gttctgtaat gcgtatttta gtcttagggg tcggcaatat tttgctgacc gatgaagcca
     6001 tcggcgtgcg gattgtcgaa gcgttagagc aacgatacat tctgccggat tatgttgaga
     6061 tcctcgatgg cggcacggcg ggaatggagc tgcttggcga catggcaaat cgcgatcatc
     6121 tgattattgc ggatgccatt gtgtcgaaaa agaacacgcc gggaacgatg atgatcctgc
     6181 gggatgaaga agttccggcg ttgtttacca acaaaatctc tccgcatcag cttggcctgg
     6241 ccgacgtatt gtcggccctg cgcttcaccg gtgagtttcc gaaaaagctg accctggtcg
     6301 gcgtgatccc ggaatcgctg gagccacaca tcggcttaac gccgacggtt gaagcaatga
     6361 ttgaacctgc gcttgagcag gttctggctg cgctgcgcga atcaggcgtg gaagccatcc
     6421 cacgggaggc gattcatgac tgaagagata gcaggtttcc agacctcccc gaaggcgcaa
     6481 gtacaggcag cgtttgaaga aattgcccgg cgttcgatgt acgatctctc ttttctgcat
     6541 ccttcaatgc cggtgtatgt ttctgatttt acgctgttcg aaggtcagtg gacggggtgt
     6601 gtgatcaccc cgtggatgct gagtgcagtt atcttccccg gcccggatca actctggccg
     6661 ctgcgcaaag tgagtgaaaa aattggtctg caactgccgt atggcaccat gacctttacc
     6721 gttggcgaac tggacggtgt ttcgcaatac ctctcctgtt cgctgatgtc gccgctttcg
     6781 cacagcatgt cgattgaaga aggccaacgc ctgacggatg actgcgcacg aatgatcctt
     6841 tcgttgccag tcaccaatcc ggatgtacca cacgcagggc gtcgcgccct gctgtttggc
     6901 cgcaggaggg gcgaaaatgc atgagttgtc gctttgccag agcgccgttg aaattatcca
     6961 acggcaggcg gagcagcacg atgttaagcg cgtcaccgcc gtgtggctgg aaattggcgc
     7021 gctctcctgc gttgaggaga gcgccgtccg ttttagtttt gaaattgtct gccgcggaac
     7081 ggtggcgcaa gggtgcgatt tacatatcgt ctataaaccc gcccaggcct ggtgctggga
     7141 ttgcagccag gtggtggaga ttcatcagca cgatgcgcag tgtccgctct gtcacggcga
     7201 gcggttgcgt gtcgataccg gcgattcgct gatcgtcaaa agtattgaag ttgaataaga
     7261 ctgaggacaa cgcctaaaaa gcgcacggac ggtcccctcg cccctccggg gagagggtta
     7321 gggtgagagg gaacacccgc taaaggttct ttttcgcgtt tgtccggcaa tcgaaataac
     7381 cggagttaac aatgtgtatt ggcgttccag gccaggtgct ggctgtcggt gaagatattc
     7441 accagcttgc gcaggttgaa gtatgtggta tcaagcgcga cgtgaatatc gccctgattt
     7501 gcgaaggtaa ccctgccgat ctgctgggcc agtgggtgct ggtacacgtc ggatttgcca
     7561 tgagcatcat cgacgaagat gaagccaaag ccacattaga cgcactgcgc caaatggatt
     7621 acgacattac cagcgcgtga tgattagctt ttgtaggcct gataagacgc ggcagcgtcg
     7681 catcaggcat tgtgcacgat tgccggatgc ggcgtgaacg ccttatccgg cctggatggc
     7741 ttgctgcgac gaacaccaac ccttacccct gacgcttatc ttctgtattt gtctcaaaat
     7801 cactggcgtc atggcgctca tgcaactgct cgttcagcgg cccgttggtg cggttaacaa
     7861 tacgcccacg tttcactgcc ggacgttcgc ccacttcttt cgcccagcgt tgcacatgtt
     7921 tataacttcc cgcatccaga aactctgcgg catcatacac gccgccgagc accacgttgc
     7981 caaaccacgg ccaaatcgcc atatccgcaa tggtgtactc atcgcccgca acaaacttat
     8041 gctgcgccag ttgcttatcc agcacgtcga gcaggcgctt ggcttccatg gtaaagcggt
     8101 tgatggcgta ctcaattttc accggcgcgt aatggtaaaa gtgaccaaaa ccaccgccga
     8161 ggaacggcgc ggcaccctgt aaccagaaca gccagttcaa cgtttcagtc cgttttgcca
     8221 gatcctgcgg caggaagtag ccaaatttct ccgccagata aagcaggatc gaaccagatt
     8281 caaacacgcg gatcggcgga ttatgcgtat gatcgcgcag cgccgggatc ttcgagtttg
     8341 ggttcacttc gacaaagccg ctggagaatt gatcgccatc gccaatacga atcagccagg
     8401 cgtcgtactc tgcaccagta acgcccagcg ccagtagctc ctcaagcata atcgttactt
     8461 tctgaccgtt cggcgttccc agcgaataaa gttgcaatgg atgtttgcca acgggcagcg
     8521 ttttttcatg cgtcggacca gaaaacgggc gattgatatt ggcgaacgcg ccgccagcgg
     8581 atttatccca cgtccagact ttcgcgggct gataagtatt gtctgtcata gtgagttgcc
     8641 ttctgagtgg tagtgttgaa gcagtgtagc aggtcacttg ctgtacagaa tgtgaatttt
     8701 ttaacccttt gcgccagccg ctgcgatgag gtgtatgatt agtctgacct ctgtccggtt
     8761 aatgtcgctg agcaggtaat cagagcacaa caattacgcc aactgcttag gttgtattgt
     8821 taaaggtaaa gtgatgagca aaggaacgac cagccaggat gccccgttcg ggacattatt
     8881 gggctacgcc ccaggtgggg tagcaatcta ctcttcagat tacagttctc tcgatccgca
     8941 ggaatacgaa gatgacgccg tattccgtag ctatatcgac gacgaatata tgggccacaa
     9001 gtggcaatgc gttgaatttg ctcgccgttt tctctttctg aattacggtg tggtctttac
     9061 tgacgtgggt atggcgtggg agattttctc gctgcgcttc ctgcgagaag tggttaatga
     9121 caacatcctg ccattgcagg catttcctaa cggctcgccg cgtgcgccgg tcgcgggtgc
     9181 gcttcttatc tgggataaag gcggtgaatt taaagacact ggccatgtcg ccatcattac
     9241 ccaattgcat ggcaacaaag tccgtattgc ggaacagaac gtgattcatt ccccgttgcc
     9301 gcaagggcaa cagtggacgc gcgagctgga gatggtggtc gaaaacggct gctataccct
     9361 taaagacact tttgatgaca ccaccattct gggctggatg atccagacgg aagatactga
     9421 atacagctta ccgcagccgg aaattgcagg cgagctgctg aaaatcagtg gagcgcgtct
     9481 ggaaaacaaa ggccagtttg acggtaaatg gctggatgaa aaagatccgc tacaaaacgc
     9541 ctatgtgcag gccaacggtc aggtgatcaa tcaggatccg tatcattact acaccattac
     9601 cgagagtgcc gagcaggagc taattaaagc caccaacgag ctgcatctga tgtatcttca
     9661 cgcaaccgac aaggtgctga aagatgacaa cctgctggcg ctgttcgaca ttccgaaaat
     9721 cctctggcca cgtttgcgtc tctcctggca gcgtcgccgt caccatatga tcactggtcg
     9781 tatggatttc tgcatggatg agcgtggcct gaaggtttac gaatacaacg ccgactccgc
     9841 ctcctgtcat accgaagcgg gcttgatcct cgaacgttgg gcggagcagg gctataaagg
     9901 caacggcttc aatccggcgg aagggctgat taacgaactg gctggtgcct ggaaacacag
     9961 tcgtgcacgt ccgtttgtcc atatcatgca ggacaaagat atcgaggaaa actatcacgc
    10021 gcagtttatg gagcaggcgc tgcaccaggc gggctttgaa acgcgtatct tgcgcgggct
    10081 ggatgaactg ggctgggatg ctgccgggca actgattgat ggggaagggc gactggttaa
    10141 ctgcgtgtgg aaaacctggg cgtgggaaac cgcgtttgat cagattcgtg aagttagcga
    10201 ccgtgagttt gctgcggtgc caatccgtac cggtcatccg caaaacgaag tgcgtcttat
    10261 cgacgtattg ctgcgcccgg aagtgctggt ctttgagccg ctgtggacgg tgatccccgg
    10321 caacaaagcg attctgccga tcctctggtc gctgttcccg caccatcgtt acctgctgga
    10381 taccgatttc actgttaatg atgaattggt gaaaacaggt tacgcagtga aaccgatcgc
    10441 cggtcgctgt ggtagcaata tcgacctggt cagccatcat gaagaggtgc tggacaaaac
    10501 cagcggtaaa tttgccgagc agaaaaacat ctatcagcaa ctgtggtgtt tgccgaaagt
    10561 ggacggtaaa tacattcagg tatgtacctt caccgttggc ggcaactacg gtgggacgtg
    10621 tttgcgcggt gatgaatcac tggtcatcaa aaaagagagt gatattgaac cgttaattgt
    10681 ggtgaaaaag taatacatgt tgtagcaacc ccgtactgat tatgaagaat aattagtacg
    10741 gggattaatc aaaatattca atatttatat attccaatat ttattcattt catatgtgaa
    10801 tatattaacc agtggaatac ctgtgttttg attatttcta aaggttttga atgaatgctt
    10861 attgtctgat acacaagaaa taacactctt tttatcgtta aaaaatgata tttcactttg
    10921 cccatgccgt taaagtcact gataatgcgt ccgttcgtaa attcaaaatg gcgtaatcta
    10981 atatatgcta aatttatttg ttggccttga tatatatacg gggcttttgt tattacttgc
    11041 tctggcattt gtgttgttct acgaagcaat caatggtttt catgacacgg cgaatgcggt
    11101 ggcaaccgtt atttatactc gtgccatgca gccacaactt gctgtggtga tggcggcatt
    11161 ttttaacttt tttggcgtgt tattgggcgg acttagcgtt gcctatgcca ttgtccatat
    11221 gttaccaacc gatttgttgc tgaatatggg gtcaactcac ggtctggcga tggtcttttc
    11281 catgctgctg gcggcgatta tctggaacct gggaacgtgg ttctttggtt taccggcctc
    11341 cagttcgcac accttgattg gtgcgattat cggcatcggt ttaaccaacg cgctgttaac
    11401 cggctcatcg gtgatggatg cgttaaacct gcgtgaagta accaaaattt tctcctcgct
    11461 gattgtttcc cctatcgtcg gcctggtcat tgcgggtggt ctgattttcc tgctgcgcag
    11521 tttctggagt gggacgaaaa agcgtgaccg tattcaccgc attccggaag atcgcaaaaa
    11581 gaaaaagggc aaacgtaaac cgccattctg gacgcgtatt gcgctgattg tttccgctgc
    11641 gggcgtggcg ttttcgcacg gcgcgaacga cgggcagaaa gggatcggcc tggtaatgct
    11701 ggtactggtg gggattgccc ctgctggctt cgtcgtcaat atgaatgcgt ccggctatga
    11761 aattacccgt acccgcgatg ccgttaccaa cttcgaacac tacctgcaac agcatcctga
    11821 actgccgcag aagttgattg cgatggaacc tccattgcct gcagcatcga ctgatggcac
    11881 gcaagtaaca gagtttcact gtcatccggc aaataccttt gatgcgattg cgcgcgttaa
    11941 aacgatgctg ccaggcaata tggaaagtta cgagccgtta agcgtgagtc agcgcagcca
    12001 gctgcgccgc attatgctgt gcatctctga tacctccgcg aagctggcga aactaccagg
    12061 cgtcagtaaa gaagaccaga acctgctgaa aaaactgcgc agcgatatgt taagcaccat
    12121 tgagtacgct ccggtgtgga tcatcatggc ggtagcactg gcgctcggca ttggcaccat
    12181 gattggctgg cgtcgtgtag cgatgaccat cggtgagaag attggtaagc gcggcatgac
    12241 gtatgcgcaa ggcatggcgg cacaaatgac ggcggcagtg tctatcggcc ttgccagtta
    12301 tattgggatg cctgtctcca caacacacgt cctctcgtct gcggtagcag ggacgatggt
    12361 ggtggacggc ggtgggttac agcgtaaaac ggtaaccagc atcctgatgg cgtgggtgtt
    12421 tactttaccg gcggcaattt ttctttctgg tgggctgtac tggatagcat tgcagttgat
    12481 ttaaccttcc tgaaaatgcc cggtcctggc gatcgggcat ttccatttt
//
LOCUS       DADLSB010000111        12425 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.111, whole genome shotgun sequence.
ACCESSION   DADLSB010000111 DADLSB010000000
VERSION     DADLSB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.111"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(74..4534)
                     /gene="mukB"
                     /locus_tag="JN331_002954"
     CDS             complement(74..4534)
                     /gene="mukB"
                     /locus_tag="JN331_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY7261051.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADRGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(4534..5238)
                     /gene="mukE"
                     /locus_tag="JN331_002955"
     CDS             complement(4534..5238)
                     /gene="mukE"
                     /locus_tag="JN331_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY7261052.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5219..6541)
                     /gene="mukF"
                     /locus_tag="JN331_002956"
     CDS             complement(5219..6541)
                     /gene="mukF"
                     /locus_tag="JN331_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY7261053.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6538..7323)
                     /gene="cmoM"
                     /locus_tag="JN331_002957"
     CDS             complement(6538..7323)
                     /gene="cmoM"
                     /locus_tag="JN331_002957"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY7261054.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRLGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7459..8238
                     /gene="elyC"
                     /locus_tag="JN331_002958"
     CDS             7459..8238
                     /gene="elyC"
                     /locus_tag="JN331_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY7261055.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8215..9108)
                     /locus_tag="JN331_002959"
     CDS             complement(8215..9108)
                     /locus_tag="JN331_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY7261056.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9262..10008)
                     /gene="kdsB"
                     /locus_tag="JN331_002960"
     CDS             complement(9262..10008)
                     /gene="kdsB"
                     /locus_tag="JN331_002960"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY7261057.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEIR"
     gene            complement(10005..10187)
                     /locus_tag="JN331_002961"
     CDS             complement(10005..10187)
                     /locus_tag="JN331_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY7261058.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10239..11471)
                     /locus_tag="JN331_002962"
     CDS             complement(10239..11471)
                     /locus_tag="JN331_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY7261059.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11508..>12425)
                     /gene="lpxK"
                     /locus_tag="JN331_002963"
     CDS             complement(11508..>12425)
                     /gene="lpxK"
                     /locus_tag="JN331_002963"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY7261060.1"
                     /translation="GLVSGAIRLCYKLKLKRAWRAPVPVVVVGNLTAGGNGKTPVVVW
                     LVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTTTAQAGDEPVLIYQRTDAPVAVSP
                     VRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVEIVVIDGVRRFGNGWWLPAGPMRE
                     RAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNLRTGTRCDVAQLEHVVAMAGIGHP
                     PRFFATLKMCGVQPEKCVPLADHQSLNHADVSALVSAGQTLVMTEKDAVKCRAFAEEN
                     WWYLPVDAQLSGDEPAKLLTQLTSLASGN"
BASE COUNT         2865 a         3537 c         3085 g         2938 t
ORIGIN      
        1 ttatgtgcca tttttttagt gcagaaacgg agttttcgga aaaagaaaag gcggcattgc
       61 tgccgcctta attttaactc gcctgggaag gcgcttcgtc acttcctgga agcgtttcag
      121 ggagttgcgg cgcaaatcct cgcaggccga cgacgtgaac atgttcggta ttctggaaga
      181 ctttacgtac cagtttatag gtggtgcctt tttccggact gatattttcc ggcgctgcga
      241 tgatgagctg catttgcaaa cgttcacaca attcaaacag cgtggcgata gaacgagcat
      301 ccagtcgcgc tgcttcatcg aggaacagca ggcggcaagg agagatatct ttaccgcgca
      361 ggcggcgaga ttcatcttcc cagctttgta ccaccatcac cagaatcgac ataccggtac
      421 caatcgcctc accggtcgac aatgcaccag actctgcgcg caaccaacca tcggaaccac
      481 ggttaacctc aacttccatt tccagatagt tgcggtaatc cagcagttct tcgccaatgg
      541 tctgcggcgt gcgctgcccc atatcaatct gcggattaag acgctgatac aatttcgcca
      601 acgcttccga gaaggtcaga cggttgctgt taaacagatc ctgatgctgc tcgtgctgtt
      661 cagagagcac atccagcagc atggcgtgcg tttcacgcac gttcacgttg agacgcacgc
      721 tgttcacctg accaaacgat acgttctgca acccctggtt gagcatacgg atacggttct
      781 gctcgcgctg aatggttttg cgaatgatgt tcgccacgct gcgggaactg atcgccagtt
      841 tctgttcacg ggaggttaat tcttcggtca gacggctaag ttcaatctcc atctgttcga
      901 tagcttccac cggatcatcg gtacgaataa tatcctgacg aatacgttca cgcagatgct
      961 gataaaccgc cacgaagaac tgaattttac gctccggacg tttcggatct tccgacatgc
     1021 gcagcacgtc gcgcagatgt tcgttatccg ccaccgccag acgcagcgca cctaacgcct
     1081 tatccgacat ggaacgcaaa tcatcagcgg agagataagc cagctcacga cggtgtaagc
     1141 ggcgctcaac gccgttatct ttcaccatgc gcatcaccgc acaccagccc gctttggcgg
     1201 tcactacctg ctcgcgcatc tcaaagtaat cccgctccag cttgcgcagt ttgcgggtca
     1261 ggttgtccat ctccgcttcg cagaaggtaa gcgctttttc cagttgattg cggcgtgaac
     1321 ggttattgct cagttgcgca tgcagctcgt cacggcgaat acgcgcccgc tcttctgccc
     1381 ctctatcagc acgcacgccg atatcctgca attcacgttg cagatcgttg agtagctctt
     1441 ttttggtgtc gtaagaactt ttcagcgaag ccagcacctg gttgtactga ctcaactgcg
     1501 ctgcgtgacc gcgcaacgct tcgcgagcgc gggtacgctc cgcttccgcc tgttccagac
     1561 gttcacgcag tttttcgttg agatcgctgt taccgctaag catttctgcc gagtcagaat
     1621 agctaaagtg cgcacgacgc tgcaccactt ccgtcagggc aaacgcttgc tgacgggcat
     1681 cgcgctgcat ctgctgagag tacgcgtaat cttcttttaa ctgttcgaac tgttccgggt
     1741 cgctctgcaa taccgaaacg atcggttcca gtttcgccag ttgattgcca aactgctgaa
     1801 caaaacgcgc ggcttcctgg gcttcatcca gacgttcgcg gatttcatcg acgcgatccg
     1861 ccaggctgtc atcagccaac aggttgagac gcggcagaat gcggttcagc gccgtaacgc
     1921 cttctttcgc ctgctcaaac tgaatacgct gctgctggtt atcattttca tgattactca
     1981 acgcccgctc cagttcgact cgacggctgt tcagttgacg gatttctgct tccgggtcag
     2041 actcaaacgc caccgccaga tgactgccga taaagcggct gaacgcctga tgcagacgct
     2101 gggttttctg tacatcaaag gagagcgtgg cgaagcgttc ggaaagcact tcacgctcgg
     2161 catggaggct ttcaatacgg ctttcacgcg cagcacgacc aaacagcggc acttccggga
     2221 aacgtgaata acgccactgt cgatcggcga ttttcaccac caccgctttt tccagctcat
     2281 caacgctgaa cacgctatca tcgaatgact gcggatcccc ttcgatcaaa tagagatctt
     2341 ccgggcaatc ggtcaagcct tccaggtgtt cggttacctg tgacagatct ggcaccacga
     2401 tggcgtggcg tgacgggcca tacagagccg agaagtacgg cgcatcttcc aggctaacgt
     2461 cgtcataaat ttctgacagc agcacaccac caaaacgctc cgccagcgcg ttcagacgct
     2521 gatcttcaga gccgccaggc tggcttaaac gttcgatctc ttcatcgacg gcgtttttgc
     2581 gcgcgcctac ttcatcgcgt tcaacaatcg cctcgcgctc acgctccagc aactgttgca
     2641 gatattcagt gacgtcctgg ctggaggaga actcttcgcc gcactgttca ctcaactggt
     2701 tgagactgtt ttgcgctgcc agccaaaccg gtgcacgctg catcaaactc tgaatgcgag
     2761 actgcagctg ttcctgttcc tgccgcagtg ccatccgctc ttcacgggcg ttagatacgc
     2821 tatcggaaag agaggcaatg cgtgcttcca gctcctgatg cagagcttcc agttcatcaa
     2881 tatcaaaatt cttgccctga cgtttgcaga aatctgccag cagacgctca gcttcttgct
     2941 gctcacgcag acgctgttcc agttcgctta agcgcatccg caacggctga acctgctctg
     3001 ccaggtggcg ctgatcaacc ccttcgcgca atagttcgcg agcgacatcc cacgcctcgt
     3061 tacgcgccag cgggccgttg attgccacca ccagctgata agcctgctca aactggctgt
     3121 gcgcggtttg cgccatgctc attttctgct caagagagag cattttttca gtcgcttcca
     3181 gctctttcgc ctggaaggtt tccagccatt cggcggcgct gtcggcggtt aagtccggca
     3241 gatggcacag ttctttggca cgattaagcg cagcaatcgc ctggttatac tggatcgcgc
     3301 gcgtttgctg gacgtccagc gcctgctggt agtcagcaag ctggcttttc agctcgtcca
     3361 cttccagttc ggcagcttcc gcgcgagcct cattctcttc ctggcgttcg atggcttctg
     3421 ccaccacttc attttgctct tccagacgga tctgcagctc atcgagatcc acttcgtagc
     3481 gttcgatctt ctcctgctga cgcaatgcgg tttgcaccag gttcaggtga tcgctggccg
     3541 cctgataatc cgcttccaga tcgccttcgg caccgttgtg ctctgccagc tcacgcgcca
     3601 tatcgacgtg tttgtactgc tcagccgcca gttgctgacg cgaagtatgt agctcgcgac
     3661 gaaactccag ggctttgtcg agatggacac ggcgctcgtt ggcgtgacgc atgtagtccg
     3721 ccgccacgta gttggtagct tcgctgatca gatgcttaaa caggtcacgg tccgactggg
     3781 tgacacgaat cgcttccagc gtcatgcggt tttcacgcag cgccgcttcc atgtcctgga
     3841 acgctttacg cacgccgctg ttttccggca acaggtagtc gcgcagagaa cgggtaatgg
     3901 cactggagat cccgccatac agcgaagctt cgatcagacg atagaatttg ctacggtcag
     3961 atgcggagcg cagacgacgc gcgatgatgc ccagatcgaa catcagcgag tggtaatcgg
     4021 taatggagtt gaactgttta aactgcacgc cttccatcgc ctcgagcttg tctttcagct
     4081 cgttaagcgg cagcacgcgc gcctggcgtt cgttcagggt ttcggtcacc agctgtgtcg
     4141 gctgcaccga catcggcagt ccctgaatgg caaatggttt gatatccact ttacgatcgc
     4201 gtccggcaac ctgttgcaga cgcacaccga ccaccacgcg ctggtggcgc gagttaatgg
     4261 tgtcgagcat cgaataacag acacccgctt tcagcttacc gtgcagacct ttatcgcgcg
     4321 aaccgctggt ggccccggct tccgtagtgt tacggaaatg cagcagggtc aggtcgggga
     4381 tcagcgccgt aacgaacgcc gccatcgtgg tggatttacc cgccccgtta ccgccggaaa
     4441 gcgtcgtgac cagctcgtca aggtcaaaag ttcgggcaaa aaagccgttc cagttaatca
     4501 gcgtcagtga gcgaaattta ccgcgttcaa tcattattct tcctctccgc tatctggctg
     4561 actctcttcg gtttcatcgt tgagttgcag atgattttca atcggcattg cttcgccatc
     4621 acgaatcagg cgacgctgtg cttcacgggg atcgtcgcca gcacgcacat cggctccgaa
     4681 gcggaacacc gattcggtaa tgcggaactt gctgctgtcg tggcccataa accacaccat
     4741 gcctaaacga cgcagacggt tgagcgaaga acgtactttc tcctgcaact tctgacggtc
     4801 aacgtctgaa ccggttgaac ggttgttcac cagtttcagc agtttcgctt catcggccag
     4861 ggtgagcagt tcgtcgtaca gttcctgctg ggtgaaaatc ccctcattcg ccagccgttc
     4921 cgggctgaga tagagataac agaggatttt cccgaccatc atatccagtt ccgacaagac
     4981 ggaacgcggg ataagagtgg tggaacgtgg gcgtaaatag aagaaccctt ccggtgcgcg
     5041 aataagctca acgttataac gcgcgtaaaa ctcttccaga tattcctgaa aatccatcag
     5101 gaatgcatga ttatccagtt cgtcgaggcc aatatggcgg cctgaacgta aggcgctgtc
     5161 cagcgccgga aataacggat tcgccagcgc ctgcgccagc ttaaccggca tcacttgttc
     5221 aatatttgtc gatgacatgc gcctgtacct tggctccgta atcattaatc ggctgccatt
     5281 tcgctggcag tccggtgaaa tctgcttgcg ctacgccaag acgtaccgcc tgatcaataa
     5341 caatacgcgc aacgtcaaag tgacgtgcac gcggatactg tgacagatat tcgcgtacca
     5401 ccagaccaag atccagcggc acttgtctgg ttttgtacac ggcaagttgt tcttcgatga
     5461 tcgccgccag ctgttcgcgg atctcgttaa actcttcgta ttccagatcc tcaggaagtt
     5521 ccccagtcac ttcttcatcg cgcagtgcca tctcttcgtc acgcatatcc agcagacgat
     5581 cggcattggc ataagttagc gcccacggct catcaaaata ggtttgtacc gactgacgta
     5641 accgctgagc aaagacgcgg tttttatcca tatcgatcgc ggtacgaata aatttgtgta
     5701 cgtggcggtc gtagccaatc cacaagtcga tggattgctg gccccaactg ataatacgat
     5761 cgagtttgct ctgcagatcg aacaccagac gatcgacgaa atgcagatcg tcatgggtca
     5821 tcgtcgcatc ctgaatgcgc aacagattag cctgcaattt gtcgcctgcc gcttccagcg
     5881 tatcctgcaa ttcacgcagc gttccggaag tttccgaaag caacaattca cagctggaaa
     5941 tcgccgcccg ccagtctttg ttcagcaact gggcgatatc gtccttcacc tgctgctgct
     6001 gttcgtccat cagacgttgc gtcaggtcga tactgtcgaa aatttctgct accgaatatt
     6061 tcagtggcgc atagacatta cggtgccagt gaaattcatc accgccctct tcggcggcat
     6121 ctgctgcgcg tttgagctca cccgccacaa tcgacaactg catagaaaga cgcagcgtag
     6181 aaaactcgcg ctgacggatg tagtagtcag taatgccgat gccgagcggc gtcagacggt
     6241 aaattgcgtt cccttccgcc tgctcgctgg taaagcggtt cagcagacgt tgacgcacca
     6301 tgtcgttgat cgcgttattg gcgcgcacgc cgatggtttc gctggtttgc tcaaacgcat
     6361 cactcacatg gcggaatgca tccaccagct cgccttcact catctcacca tccagacgct
     6421 cgccgttcag cgtggcaacc gccagcagaa aagagagtcg gtctaccggc agcgagatgg
     6481 agaagtcatt ttttctggcc caggcaacca gttcggggac tgtctgggaa aattcactca
     6541 tactttatcc ttgctctgcg gtttgcgcgc ggtgacatga atataacgcc ctagggtaat
     6601 atacggttcc tgacggcaat aacgcgtttc taattcaagt aatgcttcat agcagtcgcg
     6661 ctgctggtgc ttctcgcgca gataatcatg aaacacgcga acgcctgtct tacccataat
     6721 ttgccaacca gcttcttcca gccacagata aacctgcgcc gggtcgcgtg gataatctgg
     6781 cgaaagcgtc cgttttttct ttttcggcat tcccgcctgc acgtaatcaa aattcccggc
     6841 gaccatgtta tgcatcaaca aaccatgcgc attgtagaac attaacgaca acactccgcc
     6901 tagacgcaac actgaccaca gggtctgcaa tacactgcgg ggatcggcca cccactcgag
     6961 caccgcatgg aacaatatca gatcaacggg cgtttccaaa tgcgaagcaa catcctgagc
     7021 ggcgcaatgt ataaattgca tgttgtcgct cacacctttt gcttctgccg cctgttttgc
     7081 gcggtcgatc atctgcgcag aaagatcgca taaaataacc tgatgcccgc gctcggccat
     7141 tttgattgcg gtctgccctt ctccgccgcc agcatccagc acacgcagtt tttgcgggcc
     7201 catttccgcc agcacgcgat cgagatcctg ccacagaata gcctgtcgaa gctgcccttt
     7261 ggtggtgccg taaatgttac gggaaaactt ttccgcaata tcatcaaaat tgcgatcctg
     7321 cattgctttc tccaccggtt ggctaagacg ttttctgata aagacgctat tttgtcacag
     7381 ccacggcgag aatgaacccg tctggtgtaa tatcgctggc gatcccttgc tatatggtta
     7441 aaaaaggaac cagaaaggat gctttttaca ctgaaaaaag tgattggcaa tatgttgctg
     7501 ccacttccgc tgatgttgtt aattattggc gctggcctgg cgctgctgtg gtttagccgt
     7561 tttcaaaaaa ccgggaagat ttttattagc attgggtggc tggcactctt gttattgagt
     7621 ttgcaaccgg tagccgaccg cctgctgcgt cccatcgaaa gcacctatcc gacatggaat
     7681 aattcgcaaa aagtggacta tatcgtggtg ctcggaggtg gttatacctg gaacccgcag
     7741 tgggcaccga gctctaattt aatcaataac agtctgccac gcctgaatga gggtatccgt
     7801 ttatggcgcg aaaatccggg ttcaaaactg atcttcacgg gaggcgtagc aaaaaccaat
     7861 acggtgagta cagcggaagt aggtgccaga gttgcgcaat cgctgggcgt accgcgcgag
     7921 caaattatca ccctggattt gccaaaagat accgaagaag aagctgcggc agtgaaacag
     7981 gcgattggtg acgccccttt cctgctggtg acctccgcat cacatctgcc gcgggcgatg
     8041 atcttttttc agcaggaagg gttaaatccg ctaccagccc cggcgaatca gttggccatc
     8101 gactcgccgc ttaatccgtg ggaacgggca atcccttctc cggtctggct gatgcatagc
     8161 gatcgtgttg gctatgaaac gcttggccgc atctggcagt ggctgaaagg tccgtcaggc
     8221 gagccacggc aagagtgatt ttgatgccag atcgaagttg cgccgactaa atcgtccggt
     8281 attaaccagt tgcgcaactt catcccataa cacatacaac caacgccgcc agatgaacga
     8341 ctccgccact ggcgcgcgtt gcaggtaact ccagagcaaa tcttccgcca gagaattatc
     8401 cattagtcgg aacagttcgt attctcgcgg tgcccaaagc attagtcccg gcccgaccat
     8461 cgccagtaac tgatcgctgc gcgaatcttt cagcatgctg cgtaaacaga agttaccgtg
     8521 aatcaacaca caattgtcgt taaagccttc aaataatgcc gggagacatt cgcgagtgcg
     8581 aaacaggatc cgcttatcct gcatcgtcag accggtgtta ttgaactgat tgagcgtggt
     8641 ccatagcact tcaacatgtt gccggtacca tgagggccag aaattttcct gagtattgtc
     8701 gaccgcgccg acgcaaccgc gactgtcctg acggtgccag gccagtaagg cttcaacgat
     8761 ttggtctttg agctgttccc agcgttctgg tgttcgggct ggtgcctcca ccgaaacacc
     8821 acgcatccgc tccagtagca ggacatcagg gccggggtgt tcttcatgcg tcatcacacc
     8881 ataaatagtc ggcatacgga cagtcccact tcttgccagc atggtggttt tccatgccag
     8941 ttgtcgggct tttccgggcg tactaaagct tcttgccatt aacggcattg ggtttccctg
     9001 gctgtcatac aaagcccaca acgccgtatc cgctttttca ttgacgcact caatacgact
     9061 gagtttttcg cccagtaaat ggcttaattc ggcacgcagc tgttccattc agattaccct
     9121 cataaaagct actgctttca taatgagcgc caatataaaa gatgtcacct gaatgagatc
     9181 aattgttgaa aagaaaaatt gaggaaaatc gtaacggtac gacactcctc ccaaaattgg
     9241 ctgaagtgtc gtgaagtgaa attagcgtat ttcagcgcga acgcgctcaa gatcttcagg
     9301 ggtatccaca cctgtgccag gaacttcctg agcaacagca acatggattt tttcgccgta
     9361 ccacagaaca cgaagctgct ctaacatttc gatgtgttct aacggacttg cctgccagtt
     9421 gacgtaacga cggataaagc ctgcacggta gccataaata ccaagatgac gcaggaagtt
     9481 atcgccaacg gtttcaaggc cttctgcaaa acgatcacga tcccaaggaa tggtggcacg
     9541 tgagaagtac agtgcatacc cttcagcgtc gagaaccact ttcaccgcat tcgggttaaa
     9601 cgcttcttcc gcattgtgga ttggcaccgc cagagtcgcc atacccacct gacgctgagc
     9661 gaggttatca gcaacctgac gaatgattgt cgcagggatc atcggttcat caccctgcac
     9721 attaacgatc accgtgtcgt cgctgaatgc gcatttttcg acaacttccg ccagacgttc
     9781 tgttcctgac tgatgatcgg cgcgcgtcat acatacttca ccgccagcgg cttcaacggc
     9841 gcgggcaaca tcctcatgat cggttgccac gatgatgcgc tcggcacctg attcacgcgc
     9901 gcgttcaaga acatgaacaa tcatgggttt gccgttaata tcaaccaatg gtttaccggg
     9961 cagacgcgtg gacgcgtagc gcgcgggaat aatgaccaca aaactcatga tttactctca
    10021 tcagcagtca gcacgcgggc ttccgtttcc agcaacactg gaatgccatc gcggagcggg
    10081 aaagccaggt tatccagttt gcaaataagc tcttgttttt cctggttata ccaaagtttt
    10141 ccgttgcaaa ccgggcaggc aatgatttca agcaaacgat gatccatagt tcctccagat
    10201 ggatcgggtt atgaatgcat aaatcttatc ataatcattt atgcgacggg gtctatttcc
    10261 cagccagtgc ggcaatcagt aaagagacct tgcgggcagc gtcctaatgt cacccgcgtt
    10321 gcctgctgcc agctcgcgaa atcagtaatc gcctgacgta accctttttg cagcattgtc
    10381 gttggtttaa tgccttcctg taaccacaga gagataactt caaggatgcc tgtctggcga
    10441 tgcattttgg catccattcg cccaactaat tgcccacgat gtaataatgg cagaacaaaa
    10501 tagccatact ggcgtttcgg cgctggggta tagcactcca gccggtagct aaaatcaaaa
    10561 agctgctctg cgcgtttgcg atcccagaca acaggatcaa aaggcgaaag taccgcgctg
    10621 tgcgttgcag tgagctttcc ggccagcgca cgctcgagta gcggcagcaa atcagcatgc
    10681 agccaaagat tgcccaattt ttcaacatgc acagcaatga tttgctgctg ttcagccctc
    10741 gcttcgcgcc acgccgccag tgccggacgt ttcagccgat agtaatctgc cagccactgt
    10801 tcgcggaata ttcccaggct acgcgcactg ttatccagca tgatgatttc tgcttctgtt
    10861 tgcgaaacga gatcgcgctc atcatcccag tcaggcatga cacggtgggt taaatcataa
    10921 acgcgctgga agttgcgccg ttcaatcacc atcacctttc cggcagtaaa taaaccttcc
    10981 agatgccgtt tatgcggctt ccattcccac cagccgcttg cacctttacg aggatgctca
    11041 aaatcggctg aacgtaccgg ccctttatca tgaatatgct gaattaactg tgcaatttcc
    11101 gcctcatgtt cctgcatcca ggcgtctttg tatttccagc ccattttttc aggtgccagc
    11161 atgcggtggc gaataagacg aaagtcgcta cgcggcataa agcaggcttc atgcgcccag
    11221 tattccatta attcgccacg cgccagagac tcatccagcc actgggcagg ataatttccc
    11281 agacgactga aaagcaccag atatggacta cgggcaacaa tattgatggt atcgatttgc
    11341 agcaaggaca tgcgggagat cgttgccgga atatcctcca acgacgctcg acggcggggt
    11401 ttgtttaaca ggccttgtgc ggcaaggtga agattacgcg catcagcaag ggagaggtgc
    11461 ggcagcgaca ttcatgactc catcaatcga acgctgccgc ggcgtaacta gttgccagaa
    11521 gccagcgagg ttagttgcgt aagcagtttc gctggttcat cacctgaaag ctgtgcgtct
    11581 acaggcaaat accaccaatt ttcttctgca aaggcccggc atttcaccgc atctttttca
    11641 gtcattacca gcgtttgccc ggcgcttacc aacgcactga catccgcatg gttcaaagac
    11701 tgatgatcgg ccagcggtac acatttttcc ggttgtacgc cacacatctt cagcgtggca
    11761 aaaaagcgcg gcggatgccc aatccccgcc atcgccacta catgttcaag ctgagcaacg
    11821 tcacaacgcg taccggtacg taaattcacc gcctgacccg gcagcagatg catggggatt
    11881 tcaccgctgc gagggacacc gccgttgacg attaccgcat caaccgactt taagcgcccc
    11941 gctcgctcac gcattggccc cgccggcaac caccagccat tgccaaagcg acgcacacca
    12001 tcaataacga caatttccac atcacgcgcc agacggtaat gctgtaaacc gtcgtcggtt
    12061 acgatgatct gcacatcagg gtgttgcgcc agaatggctt ttaccgcatc agaacgaacg
    12121 ggagaaaccg caacaggcgc atcagtgcgt tgataaatca acacaggttc atcacccgcc
    12181 tgtgctgtgg tggtatctgc cgacaataac agcggataag attcagcctt accaccatat
    12241 ccccgcgata cgacccccac gcgaataccg cgctgttgca actgttccac cagccagaca
    12301 acgaccgggg tttttccgtt gccgcctgcg gtgagattac caaccacgac aaccggtacg
    12361 ggggcacgcc aggcgcgctt cagttttagt ttatagcaaa gacggatcgc gccactcacc
    12421 aggcc
//
LOCUS       DADLSB010000112        11972 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.112, whole genome shotgun sequence.
ACCESSION   DADLSB010000112 DADLSB010000000
VERSION     DADLSB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.112"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..177
                     /locus_tag="JN331_002964"
     CDS             <1..177
                     /locus_tag="JN331_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7261061.1"
                     /translation="QTERLLTWRGMYQQLLKVDPLKCELCGGQMRFTGLKMGYRLAEQ
                     IMMHEPLARMRWCG"
     gene            326..611
                     /locus_tag="JN331_002965"
                     /pseudo
     CDS             326..611
                     /locus_tag="JN331_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1013..1549
                     /locus_tag="JN331_002966"
     CDS             1013..1549
                     /locus_tag="JN331_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7261062.1"
                     /translation="MKYIVFVSALLISNTGKGGGQRVVFNVDIVGSACHVVVDADGFG
                     NSGRLIFGTYRKSSGVPVLPRDFVVRLYESGATVQGCSAFRAGQVATLDFGNPGQLDA
                     KGVVTRGAGDGIRVDVRAVDTQADYRGRLTQDSHSVNYPVEFAVKGQFRFRAQPVFPA
                     NVKAGEYTGALTFVVTYQ"
     gene            1564..4002
                     /gene="faeD"
                     /locus_tag="JN331_002967"
     CDS             1564..4002
                     /gene="faeD"
                     /locus_tag="JN331_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial usher FaeD"
                     /protein_id="HAY7261063.1"
                     /translation="MCAGKKVVAELVSSLFSRRAALAVSAAFASTTGVAGEKLDMSFI
                     RGGAGVNPEVWAALNGSYAPGRYLVDLSLNGKEAGKQILDVTPQDSDELCLTEAWLAK
                     AGIYVSADYFREGYDATRQCYVLTKAPSVKVDFDVSTQSLALSIPQKGLVKMPENVDW
                     DYGTGAFRVNYNANANIGRNNTSTFGSADLKANIGHWVVNSSASASGGDSGDNSATIN
                     AFTATRAIRALSADLTVGKTSTGSSLLGSTGTYGVSLSRNNSMKPGNLGYTPVFSGIA
                     NGASRVTLTQNGRLLHSEMVPAGPFSITDVPLYTSGDVTMKITGEDGREQTQLFPLSV
                     MAGQLNPGQHEFSVAVGLSDDDSDLKGGVFAASYGYGLDGLTLRTGGVFNRDWQGISA
                     GAVLGLSYLGAVSADGAYAMAEYRDGSRSGNKVQLSWSKQLEMTNTGLRVSWSRQSEE
                     YEDMSSFDPTELWSQSNHGRRPKDEWNAGISQSVGGLFSLSVSGWQRSYYPAQMTGRY
                     RYSDDNGLDTGITGTLSTQIKGVSLNLGWSGSRNSQGENNRSASASISVPFTLFERRY
                     SSSTSVSTSKGGGTGFSTGVSGSLNDRFSYSLGGGRDSGGGVSSYLNASYSGDRAYLN
                     GALNHSQSGGTSGSVSVSGSVLVVPMAKDIMFSRTTGDTVAVVNVKDTPGVKVTSGDG
                     QTDSDGNLVVPLNSYDWNTVTIDAGTLPLSTELTNTSQKVVPTDKAVVWMPFDALKVK
                     RYLLQVKQRDGEFVPGGTWARDSKNTPLGFVANNGVLMINTVDAPGDITLGQCRIPAA
                     RLQDTEKLQEITCE"
     gene            3995..4777
                     /gene="faeE"
                     /locus_tag="JN331_002968"
     CDS             3995..4777
                     /gene="faeE"
                     /locus_tag="JN331_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial chaperone FaeE"
                     /protein_id="HAY7261064.1"
                     /translation="MSKRNAVTTFFTNQVTKALGMTLALMMSCQSAVASLAADQTRYI
                     FRGDKDALTITVTNNDKERTFGGQAWVDNIVEKDTRPTFVVTPSFFKVKPNGQQTLRI
                     IMASDHLPKDKESVYWLNLQDIPPALEGSGIAVALRTKLKLFYRPKALLEGRKGAEEG
                     ISLQSRPDGRTMLVNTTPYIFAIGSLLDGNGKKIAADNETAQKLLMFMPGDEVQVKGN
                     VVKVDSLNDYGELQTWTINQKKSAISSAQKSSDTEGNSSDKK"
     gene            4812..5303
                     /gene="faeF"
                     /locus_tag="JN331_002969"
     CDS             4812..5303
                     /gene="faeF"
                     /locus_tag="JN331_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeF"
                     /protein_id="HAY7261065.1"
                     /translation="MKKTMMVVALALSALSIQSTLAAEYSEKAQYLGVVNGQVVGNSV
                     VKVTRIPTDPVLYRSGDTTPLPDRLTIRNAESRAASGGLAYITVKQVLPDNGEARITL
                     KTALMVDGKKVAISARQQGEDMVITLPEAQKQIELRTDAPAELEVPVSYRGNLQIALQ
                     VED"
     gene            5541..6341
                     /locus_tag="JN331_002970"
     CDS             5541..6341
                     /locus_tag="JN331_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cshE pilin"
                     /protein_id="HAY7261066.1"
                     /translation="MKKTLIALTVAASAAVSGSAMAWNPSGLGGNGGTFSMSGTLSKA
                     EKTIPWAVEIGAPVTALDVTVKPDQKNVFILLEKDIPILGIRSNPTGFMGEKGIAPQI
                     DYNKAADVDYMDKGNLPAQVNVTSSDGKKIGTLAFVMRVACQASEKRGEGDDKLESNM
                     LFASSKGKAFFGGVAKDDKGVWSDAAAYTWATHLFSSIANSWDTKIPYTAGDTDETDF
                     SNGDRLYRGYYASGLAKDIPMELVLDQTITSATPVPWKASLPIVISYM"
     gene            6562..7359
                     /gene="faeH"
                     /locus_tag="JN331_002971"
     CDS             6562..7359
                     /gene="faeH"
                     /locus_tag="JN331_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeH"
                     /protein_id="HAY7261067.1"
                     /translation="MKITHHYKFFLSAVILVTIFYSAVPYANILDGGEIQFNGYVTDE
                     APKWAWQISSPDQTWSVDTADARTENGQLVFNLHDKGALPFLEGHLHDVAERGGPGFT
                     PFITFSSNGQPFTVTEGNGASAQHFRASVPVRDPETGNVSGQLFFTLNQGMAVSTGRQ
                     DDGASVPAGMSLVSGQSVTDVQPGSLPQGLKARLSSLLLMNQNFGNGMNAVDNGQVIN
                     QGILTDGRVMDLAAAYASEVSDFELRLPAEGTPAAWQAGLNVTVTVQ"
     gene            7389..8141
                     /gene="faeI"
                     /locus_tag="JN331_002972"
     CDS             7389..8141
                     /gene="faeI"
                     /locus_tag="JN331_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeI"
                     /protein_id="HAY7261068.1"
                     /translation="MKKVTLFLFAASHLPSCVLAWNTPGENFSGELKLEGPVTSTRNP
                     WVWKVGQGNGGLEVKQSGGGGGIQVALPAMTVLLGKTTLTTPAGREGLSPRVSYGKGT
                     EGFSLVWTAPGMAEVTLPVTGDNNARAGTFVFRIQAVGVLRHVQGGQPVYVGMYDDLN
                     TNGLPGESTAMKVSDIPGVLQKMFSGEGPGWLQTMTLSGYSGVSHFSDDFLRQVEGVY
                     GAQTVAGSGELRLNGEMPEHWRVSLSVNIEYQ"
     gene            8466..9692
                     /locus_tag="JN331_002973"
     CDS             8466..9692
                     /locus_tag="JN331_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7261069.1"
                     /translation="MSGTIPKIKTGENVLSAAIQRIEWLFGTFPSVCLSFSGGKDSTV
                     LFHLVSEVARKKRRRFSVLFIDWEAQYQCTITHIQKMQKMYLDVTDRFYWVALPLTTV
                     NGVSQFQPEWICWEPGVTWVRHPPEYAITDMAYFPFYRYAMTFEEFIPAFSSWFARDK
                     YLVAVLTGVRADESLNRFMGLVSRRKQRYADDKPWTTASPEGFYYTMYPLYDWKARDI
                     WIYNARTRAIYNPLYDLMHRACVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCE
                     RVSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAV
                     YLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYE
                     RYLQRMKERRKEWGIL"
     gene            9677..10315
                     /locus_tag="JN331_002974"
     CDS             9677..10315
                     /locus_tag="JN331_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY7261070.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            10543..10935
                     /locus_tag="JN331_002975"
                     /pseudo
     CDS             10543..10935
                     /locus_tag="JN331_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10954..11427
                     /locus_tag="JN331_002976"
     CDS             10954..11427
                     /locus_tag="JN331_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7261071.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
     gene            11579..11841
                     /locus_tag="JN331_002977"
                     /pseudo
     CDS             11579..11841
                     /locus_tag="JN331_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904911.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         3036 a         2531 c         3382 g         3023 t
ORIGIN      
        1 cagacggagc gcttactcac atggagaggg atgtatcagc agttactgaa ggttgacccg
       61 ctgaagtgcg agctgtgcgg aggtcagatg cggtttacgg gactgaagat gggttaccgt
      121 ctggcagagc agattatgat gcatgagccg ctggcacgaa tgcggtggtg cggctgagcg
      181 ccgcagaggg gaagttgcgt ccatgttgag gggaacggag caaaaaacca tcagagatat
      241 attgaatcaa tcaatgtaag caatatcaag tgtctatacc aattacgtga tgatcaggtc
      301 gtgttgaata aacgcgcatt cttcggtgct tttctaatct tttggggatt taagttcctg
      361 tcgatgaact gctggcgctg cttccccctt ttaccatcgg tatgttgacc actgacgaat
      421 ggggtagtta caccagagag ttaccgaaag agaagtatct gactggtaag atttttactc
      481 agcgcattga gcgcaacaat ctgcccctga gaacccgaat caaacgcctg gctcgcagaa
      541 ccatctgttt ctctcgctct gtaaaatttc atgaaaaagt tatcggtgct tttatcgaaa
      601 aacatatgtt cctggagtca tcaccctttt cctcttggtg ttatcccatc aatatttcgg
      661 ccacgtggtt tacgtttatt attgataata atcaatgaac tgccgagtaa aaataactgc
      721 tgatatacgg gatcccatgt caactcccgc attatatatt atacctcaat agcgttacat
      781 tattaatgta tgtaaatgtt aattaaccaa agcattaatc aattgttata taattgcgtc
      841 gcaggtaaac gccaaatcaa cgcaaataca aatagtaaaa atggttcatg ttttggcact
      901 atttggtttt tttgttttta acgggtggat atttgtttat tttttttcta aattattttt
      961 atgtaatttt cataaaaatg gggtgtgagt tattaactct aaattagtga gtattaaata
     1021 tattgtgttt gtctctgctt tattaatttc caatacggga aaaggggggg ggcagcgagt
     1081 tgttttcaat gtggatattg taggttctgc ctgtcatgtg gtggtggatg cggacggttt
     1141 cggcaacagt ggtcgactga tattcgggac ttaccgtaaa tccagtggcg tgcctgtatt
     1201 gccacgtgac tttgttgtgc gcttgtatga gtccggcgct acggttcaag gttgttcagc
     1261 ctttcgagcg gggcaggtcg caactctgga ttttggtaac ccgggccaac tggatgcaaa
     1321 gggcgtggtc actcgcggtg ctggtgatgg tattcgcgtg gatgtacggg cggtggatac
     1381 acaggcggat tatcgcggac gtctgactca ggacagtcat tcggtgaatt acccggtgga
     1441 gtttgctgtt aagggacagt tccgttttcg tgcacagccg gtgttcccgg cgaacgtgaa
     1501 ggcaggggaa tacacagggg cgctgacctt tgtggtcacg tatcagtaac agaggaaagg
     1561 tgtgtgtgtg caggaaagaa ggtggttgca gagctggttt cgtctttgtt ttcccgtcgg
     1621 gctgcgcttg ctgtatcagc tgctttcgcc agtaccacag gtgttgcagg tgaaaaactg
     1681 gatatgtcct ttatccgggg gggtgccggg gttaatccgg aagtctgggc agcgctgaac
     1741 ggcagctatg cgccgggacg ttatctggtt gacctgtccc tgaacgggaa ggaggccggg
     1801 aaacagatac tggatgtgac accacaggac agtgatgaac tgtgcctgac agaggcatgg
     1861 ctggcgaagg ccgggattta cgtcagtgca gattactttc gtgagggata tgacgccaca
     1921 cggcagtgtt atgtgctgac aaaagcgccg tcagtgaagg tggattttga tgtttccacc
     1981 cagagtctgg cgctgtccat tccccaaaag gggctggtga agatgccgga gaacgtggac
     2041 tgggattacg ggactggtgc atttcgcgtg aactataacg cgaacgccaa catcggccgg
     2101 aataacacct cgacctttgg ctcggcagac ctgaaggcca atatcgggca ctgggtggtg
     2161 aattcttctg cctcagccag cggtggtgac agcggggata actccgccac gataaacgcg
     2221 ttcacggcca cccgggccat ccgcgcactg agtgcggacc tgacggtcgg gaaaacatcc
     2281 accgggagca gtctgctggg cagtacggga acgtacggag tgtcgctgag ccggaacaac
     2341 agcatgaagc cgggcaatct ggggtatacc ccggtgttca gcggcattgc gaacggggcg
     2401 tcgagggtga cactgacaca gaacgggcgg ttgctgcatt cggagatggt gccggcaggt
     2461 ccgttctcca tcacggatgt gccgctgtac accagtgggg atgtgaccat gaaaatcacc
     2521 ggcgaggacg gtcgtgagca gactcagctc ttcccgttgt cggtgatggc cgggcagtta
     2581 aacccgggac aacacgagtt cagcgtggca gtcggtttgt ctgacgatga cagtgacctg
     2641 aaaggcggcg tgtttgcggc gtcatacggt tacggtctgg atggactgac gctgcgcacc
     2701 gggggcgtgt ttaaccggga ctggcagggt attagcgccg gggctgttct ggggctgagt
     2761 tatctggggg cagtgtctgc tgacggggct tatgccatgg cggaataccg tgacggcagc
     2821 cgcagcggaa ataaggtgca gttgtcctgg agtaaacaac tggagatgac gaacaccgga
     2881 ctgcgggtga gctggtcacg gcagagtgag gaatatgagg acatgtcctc ctttgacccg
     2941 acagagctgt ggtcgcagtc aaatcatggt cgccggccga aggatgaatg gaatgccggt
     3001 atcagtcagt cggtgggggg gctgttcagt ctgtcggtgt ccggctggca gcggagttat
     3061 tacccggcac agatgactgg ccgttatcgg tacagcgatg acaatggtct ggacacaggg
     3121 attaccggta ccctaagcac acagattaag ggcgtcagtc tgaatctggg ctggtccggc
     3181 tcacggaatt cgcagggaga gaataacagg tcggcgtcag cctctatctc ggtgccattc
     3241 acgctgtttg agcgtcgcta cagcagcagt acatcggtga gtaccagtaa aggcggtggc
     3301 acgggcttca gcactggcgt gtccggctcg ctgaatgacc gtttcagcta tagcctgggc
     3361 ggcggtcgtg acagcggtgg tggtgtgagt agttatctaa acgcgtcgta cagtggtgac
     3421 cgggcttatc tgaatggtgc cctgaaccac tcacagtccg gcggaaccag tggttctgta
     3481 tcagtcagcg gttcggtact ggtcgtcccg atggcgaaag acattatgtt cagccgcacg
     3541 accggggaca ccgtggccgt ggtgaacgtg aaggatacac cgggtgtgaa ggtgacgtcc
     3601 ggtgacggac agactgacag tgacggcaac ctggtggtac cgctgaacag ctatgactgg
     3661 aacacggtga cgattgatgc gggcacactg ccgctgagca ccgaactgac gaacaccagt
     3721 cagaaggtgg tgccgacgga caaggcggtg gtctggatgc cgtttgatgc cctgaaagtt
     3781 aagcgttacc tgctgcaggt gaagcagcgt gacggtgagt ttgtgccagg gggaacctgg
     3841 gcacgtgaca gtaagaacac accgctgggc tttgtagcta acaatggtgt gctgatgatt
     3901 aacacggtgg atgcgccggg tgatatcacc ctaggccagt gccggatacc tgcggccaga
     3961 ctgcaggata ctgagaaact acaggagatt acgtgtgagt aagcgtaacg cagtaacgac
     4021 gtttttcact aaccaggtga caaaagcact gggaatgacg ctggcgctga tgatgagctg
     4081 tcagagtgcg gtggcttccc tggcggcaga ccagacccgc tatatctttc gcggggacaa
     4141 ggatgcgctg accatcacgg tcaccaacaa tgacaaggaa cgtacctttg gtggtcaggc
     4201 atgggtggac aatatcgtgg agaaagacac ccgtccgact tttgtggtga cgccgtcctt
     4261 cttcaaggtg aagccgaatg gtcagcagac actgcgtatc atcatggcct cggaccatct
     4321 gccgaaggat aaagagtcgg tgtactggct gaacctgcag gatattccgc cggctctgga
     4381 gggcagcggt attgcagtgg cgctgcgcac gaaactgaaa ctgttctatc gcccgaaggc
     4441 actgcttgaa ggtcgcaagg gggcagaaga aggcatcagt ctgcagagcc gcccggatgg
     4501 caggaccatg ctggtgaaca ccacgccata catttttgcg attggcagcc tgctggacgg
     4561 aaacggaaag aaaattgccg cggataacga gacggcgcag aaactgctga tgttcatgcc
     4621 gggtgatgaa gtacaggtga agggaaatgt ggtgaaagtg gactctctga atgattacgg
     4681 tgaactgcag acatggacga ttaaccagaa gaaatcagca atatcgtctg cacagaagtc
     4741 atctgataca gagggtaatt catctgataa aaaataatcg ccctccggca tacggaacag
     4801 aggggtaata aatgaagaaa acaatgatgg tagtcgcctt ggccctgagt gcactcagta
     4861 ttcagtcaac acttgccgct gaatacagcg agaaagcgca gtatctgggg gtggtgaatg
     4921 gccaggtggt gggtaacagc gtggtgaagg taacccgcat accgacagat ccggtgctgt
     4981 accggagtgg agacacgacg cctcttcccg accgcctgac catccgtaat gcagaatcca
     5041 gagcagcatc tggtggcttg gcgtatatca cggtgaaaca ggtgctgccg gataacgggg
     5101 aagcccgcat caccctgaag acggccctga tggttgacgg aaagaaagtg gcaatcagcg
     5161 ccagacagca gggtgaggat atggtgataa ctttgccgga agcacagaag cagattgaat
     5221 taagaacgga tgcaccggca gagctggaag taccggtcag ttaccgggga aatctgcaga
     5281 tagcgctgca ggtggaggac tgaggattca tttcctttat aaatgcagta catcccgcga
     5341 taccggagcg ggtcatattg gtaaagggat ttaccgtctt ttaaaacggg aataacgtaa
     5401 aactattctg agttaaacac tgtgtttaat gaaatgcggt tatttaaatg aatccgcagg
     5461 ggatggtttt gcggtaattc cgggaaaata aggattaccg attcagttta ttatttatga
     5521 aacgtcaagg tgattatttt atgaaaaaga ctctgattgc actgactgtg gctgcttctg
     5581 ccgcagtatc cggctctgct atggcgtgga atccctctgg ccttggtggc aacggtggca
     5641 cctttagcat gagtggtact ctgagcaaag ctgagaaaac aataccgtgg gcagtggaaa
     5701 taggtgctcc ggtaacagcg cttgatgtga ccgttaaacc tgaccagaag aatgttttca
     5761 ttcttttaga aaaagacata ccgatacttg gcattcgtag caacccgact ggtttcatgg
     5821 gggaaaaggg tattgcccca caaatcgatt ataataaagc cgctgatgtg gattatatgg
     5881 ataaggggaa tttacctgcg caagttaatg tcacatcatc tgatggtaag aaaattggta
     5941 ctctggcgtt cgtaatgcga gttgcctgtc aggccagcga aaagaggggt gaaggcgacg
     6001 acaagttaga gagtaacatg ctattcgcca gttcaaaagg taaagctttc tttggtggag
     6061 ttgccaaaga tgacaaaggt gtctggagtg acgccgctgc ctatacttgg gctacccacc
     6121 tttttagcag tattgctaac tcgtgggata ctaaaattcc ttacacggcg ggcgatacag
     6181 atgaaaccga cttcagtaat ggagacaggc tttaccgagg ttactatgca tccggccttg
     6241 ccaaggatat ccccatggaa ctagttcttg atcagactat aacgtctgct accccagttc
     6301 cgtggaaagc ttctctgcca attgttatct cctacatgta attcagcagt gttcagcagt
     6361 aagcacttac ccgtttactg ctgattaaaa cttcagaaca cagtttccgg tgacccgacc
     6421 tgaatataca gaaccggtat gtcagaacag gtgttttttc tgttggagtg tcttgtgacg
     6481 catattggtt ctgtgctttt tttaacaaaa ttattttatt gagctgtttt gttttatccg
     6541 gataaacagg aatgaatgcc gatgaaaata acgcatcatt ataaattctt cctttctgct
     6601 gttattttgg taacaatatt ttattcggca gtgccatatg caaatattct tgatggcggc
     6661 gaaattcagt ttaatggcta tgttactgat gaagcaccca aatgggcctg gcagataagt
     6721 tctcccgacc agacctggtc tgtggatact gccgatgccc gtacagagaa cgggcaactg
     6781 gtttttaatt tgcatgataa aggggctctg ccatttctgg agggacatct gcatgatgtg
     6841 gctgagcgcg gtggtcccgg attcacgcca tttattactt tcagcagtaa cggacagcct
     6901 tttactgtga cggaaggaaa tggtgcctca gcacagcact ttcgcgcctc tgtcccggtg
     6961 cgtgacccgg agacggggaa cgtgtcggga cagctttttt ttaccctgaa tcagggaatg
     7021 gcggtcagta ccggcagaca ggacgacggg gcatccgtac cggctggcat gtcgctggtc
     7081 agtggacaga gtgtgacgga tgtacagccg ggaagtctgc cacaggggct gaaagcccga
     7141 ctctcttccc tgttactgat gaatcagaat tttggcaatg gcatgaatgc ggtggataac
     7201 ggacaggtta tcaaccaggg gatactgact gacggtcggg tgatggatct ggctgcggca
     7261 tatgcctctg aggtgtcgga ttttgaactg cggttgccgg cagagggtac acctgcagca
     7321 tggcaggcgg ggctgaatgt gacggttaca gtacagtgat ggttgcaggg atataaggag
     7381 agaagagaat gaaaaaggtg acgttgtttc tgtttgctgc cagccacctg ccttcctgtg
     7441 tactggcctg gaacacgcca ggagaaaatt tcagcggaga gcttaagctg gaaggcccgg
     7501 tgaccagtac ccgtaacccg tgggtatgga aagtcgggca gggaaatgga ggtctggagg
     7561 ttaaacagag tggcggtggg ggggggattc aggtcgcact gccggcgatg accgttttac
     7621 tgggaaaaac caccctgacc acaccggcag gacgtgaggg gctttcaccc cgggtcagtt
     7681 acggaaaagg cactgagggg ttttcacttg tatggacagc gcctggcatg gctgaagtga
     7741 cgctacctgt caccggagat aataatgccc gggcaggcac atttgttttc agaattcagg
     7801 ctgtgggggt gttgcgccat gtgcagggcg gacaaccggt gtatgttggt atgtatgacg
     7861 acctgaatac gaatggtttg ccaggtgaaa gcactgccat gaaggtttct gatattccgg
     7921 gcgttctgca gaagatgttc agtggggaag gccccggctg gcttcagaca atgacactca
     7981 gtggttattc aggggtgagt catttcagtg atgacttcct gcgccaggtt gaaggcgtgt
     8041 atggtgcaca gactgtggct ggcagtggtg aattacggct gaacggcgag atgccggaac
     8101 actggagggt gtcactgtca gtaaacattg aatatcagta aggagcagga gtgcgtttta
     8161 taatccggag ataaggaacg tcggttactg taagattggg gagttgtgtt gatagcggga
     8221 gtggctggaa taagtggtat acatagataa aaattaaaac tatcactatt tgagtgagtt
     8281 ttttcctgct ctccatgggc tttatagctc acaaaaaata gaataatatt attaattatt
     8341 ctgctttttt atgggcattc ccccttgaaa aatgagtttg tgccagaata ttattaatat
     8401 gttttttgtg atttactaat caaggattgc tgaatagcat ccagtactga atattttgtt
     8461 caattatgtc aggaaccata ccgaaaatca aaacaggtga aaatgttctg tctgcagcca
     8521 ttcagcgcat tgaatggcta tttggaacgt ttccttctgt ctgcttgtcc ttttctggtg
     8581 gtaaggattc taccgtgctt ttccatcttg tgtccgaagt ggcccgcaaa aagaggcgtc
     8641 gtttttcggt attgttcatt gactgggaag cacaatatca atgcaccatt acgcatattc
     8701 agaagatgca gaagatgtac ctggatgtga cggacaggtt ttactgggtg gcccttcccc
     8761 tgactacggt aaacggtgtc tctcagtttc agccggagtg gatatgctgg gagcctggtg
     8821 tgacctgggt tcgccatcca ccggaatatg ctattacgga tatggcgtat tttccctttt
     8881 accgatatgc catgaccttt gaggaattta tcccggcgtt ttcctcctgg tttgccaggg
     8941 ataaatacct ggtggctgtt ctgaccggag tccgggcaga tgagtccctc aaccgcttta
     9001 tgggactggt atcccgtcgc aaacagagat atgccgatga taaaccctgg actacggcat
     9061 cacctgaagg gttttattac accatgtatc cgttgtatga ctggaaagcc cgtgatatct
     9121 ggatatataa cgccagaacc cgagctatct acaatcccct gtatgacctg atgcaccgtg
     9181 cctgtgtgcc gttacgcaac atgcgtgtct gtgagccttt tggtcctgaa cagcgtaagg
     9241 ggctgtggct ttatcatgtt ctggagccgg aaacctgggc caggatgtgt gagcgggtgt
     9301 cgggtgctgc cagcggggcg ctttatgcca atgagagcgg tgcctatttt gccctgcgta
     9361 agcgtatcac aaagccgcct catcatacct ggcgtagcta tgcgatgttc ctgctggatg
     9421 tgatgccgga aagaacggca gaacattacc gtaataaaat tgctgtctac ctgcgctggt
     9481 atcagacgcg gggcttcccg gatgacatcc cggatgaaca ggagaatgac ctggggagcc
     9541 gggatatccc gtcctggcga cgtatctgta agacactcat aaagaatgat ttctggtgcc
     9601 ggactctctc cttcagtccg aacaaacccc ggcactatga acgttacctg cagcgtatga
     9661 aggaaaggag gaaggaatgg gggattctgt gacaccggag atacttggca acatgattcg
     9721 tcagtatttc agtcaggtac gaactgaaaa agagagcata caggctctga atcatctgcg
     9781 cagagtgctt catgaggtca gtccttttgc acaggagcct gtggactgtg tgttgtgggt
     9841 gaaagcggat gaggtggtgg ccaacgatta caaccctaat gtcatggcac taggtgagaa
     9901 aaaactactg aaacagtctc tggagaaaga tggtttcaca caaccggtgg tggtgtcaga
     9961 ggagaagaat cattatctgg tggtggatgg ttttcatcgt cagttactgg ggcgggaagc
    10021 agatacagga aaacgcctga ggggctggtt gcctgtagcc tgtattaatc cggagagaaa
    10081 gggacaggcc gcacgtattg ccgcgaccat tcgacataac cgggccaggg ggaaacacca
    10141 gatcacttta atgtctgata ttgtcaggga tctttcacgt ctgggctgga cgaatgaacg
    10201 tattggtaca gagctgggaa tggaccagga tgaagtgctg aggctgaaac agattagcgg
    10261 gctgacagag ttgtttcagg aagaggattt cagctcggct tggacggttc gttgatgttg
    10321 ttaaatatgg gcggtatttc tgaaagttga tactttgggt agaaaaaatg ataccgaaaa
    10381 ctatggagga tgtgcttgtc aggtgactgg acaccatagg tcttaggctt tgataacggg
    10441 ataagatgag ctgcttttct cataccatca ggtgtgatga tggcgcagat ttacaaaatc
    10501 tgattgattc taaagatgta taagtaaaaa tcaagctttg atatgaaaaa acagatattt
    10561 attaataata aacctcctgt agtcccttac tcagggacgc acgcgaaaat atttaaatac
    10621 attgaaatcc ctctgccgtt tttctacttt atctatacat caggcgaacc attccacata
    10681 tctgtccaaa atactgttat ttatgtatca aaatacaatg gtatatttat taataaatta
    10741 gttccttttt ctcttttatt tgacagggat atatctgttc ttcaaagaag agatatttgt
    10801 gttgtgcgat ttacgtcaga ggaaataagt gaacataacg ttttatttga tcatgacatt
    10861 gaacgattaa aaaaaatttc caaagctcag cttatatctc ccgattatgt tttgattgat
    10921 ttttctagtg gggggggaga tgaaccctat gcaatgtcct ggttaatatc tcagtgtgct
    10981 catcagtgca ctgataataa aaagacggag acagatgcca tttacgataa agttcggagt
    11041 tcttatttgc tttcatgtat tctgaaaaaa aacaaaaatg ttgggcttat tcttcatgca
    11101 ccatcatttg tttctgtgtc tgaaaaaatt gccagaattg tgatggcaaa ttactcccgt
    11161 aactggagta attccgagtt ggccagtgcg gttttaatga gtgaatcttc tttaaaaaga
    11221 agaatgtaca aagaggtcgg ttctataagt acatttgtac ataaaataaa gcttacagaa
    11281 gctatcagga aattacgaag gacaaatact cccatttctg tcatatcatc tgagttaggt
    11341 tacagttcac catcgtattt ttctaaagtt ttctttaaat atttaaagac ataccctcaa
    11401 aacataagga agaaaaatgg tcggtaatgt caccaattag gaggacttgc agagccgctt
    11461 ttttacgata ctcaccccgt aagtaagcga taattggtaa cttaaacgcc catctgacat
    11521 agatggacac ctgagtatgg aattacagga ctggcgaaaa gaacctcgta aaaagtattt
    11581 ggtgcaaatc gttggttcct ttgctgaatt tgaacgcgcc atgcttcgtg aacggacaaa
    11641 gagtggactt gcagcggcac gccaggacgg gttggtggat ggcgactaaa gttaacaccc
    11701 cagcaacaaa aagagattgt ctcgctcgtc acatcggggc agaaaacagg tgctgatgcc
    11761 gcccgtctgt ttcgggttca cccttccacc gtggtgcgtc ttctcgccag acaccggatg
    11821 actgagatca aacagacata atctatgcgg agatttacag cagggcatgg ttcacaaatc
    11881 tgacagcgat gaattatcgg cactgtgggc agaaaatgct cgaatcatca agcccctgtt
    11941 acctcctgaa cccgccacac cacgggccgg ac
//
LOCUS       DADLSB010000113        11872 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.113, whole genome shotgun sequence.
ACCESSION   DADLSB010000113 DADLSB010000000
VERSION     DADLSB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.113"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..1209
                     /locus_tag="JN331_002978"
                     /pseudo
     CDS             1..1209
                     /locus_tag="JN331_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            1432..1929
                     /gene="ubiC"
                     /locus_tag="JN331_002979"
     CDS             1432..1929
                     /gene="ubiC"
                     /locus_tag="JN331_002979"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY7261072.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1942..2814
                     /gene="ubiA"
                     /locus_tag="JN331_002980"
     CDS             1942..2814
                     /gene="ubiA"
                     /locus_tag="JN331_002980"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY7261073.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2969..5392)
                     /gene="plsB"
                     /locus_tag="JN331_002981"
     CDS             complement(2969..5392)
                     /gene="plsB"
                     /locus_tag="JN331_002981"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY7261074.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5563..5931
                     /locus_tag="JN331_002982"
     CDS             5563..5931
                     /locus_tag="JN331_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY7261075.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            6041..6649
                     /gene="lexA"
                     /locus_tag="JN331_002983"
     CDS             6041..6649
                     /gene="lexA"
                     /locus_tag="JN331_002983"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY7261076.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6668..8047
                     /gene="dinF"
                     /locus_tag="JN331_002984"
     CDS             6668..8047
                     /gene="dinF"
                     /locus_tag="JN331_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY7261077.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            8163..8372
                     /locus_tag="JN331_002985"
     CDS             8163..8372
                     /locus_tag="JN331_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY7261078.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(8414..8929)
                     /gene="zur"
                     /locus_tag="JN331_002986"
     CDS             complement(8414..8929)
                     /gene="zur"
                     /locus_tag="JN331_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY7261079.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            9247..9501
                     /locus_tag="JN331_002987"
     CDS             9247..9501
                     /locus_tag="JN331_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261080.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            9525..10232
                     /locus_tag="JN331_002988"
     CDS             9525..10232
                     /locus_tag="JN331_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY7261081.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            10595..10717
                     /locus_tag="JN331_002989"
     CDS             10595..10717
                     /locus_tag="JN331_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAY7261082.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDRMYRF"
     gene            10953..11525
                     /locus_tag="JN331_002990"
     CDS             10953..11525
                     /locus_tag="JN331_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HAY7261083.1"
                     /translation="MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRQSILKHGR
                     MIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYNLIETNGHNVSLRSDPREMDADE
                     VFDELSRADIRILDEIYSRYGHMNRFDLANMTHLESVCPEWHDPGNSRKPIDLKEMLI
                     SEGKSEDEANRIIGKMEESQKLKEFSLQLS"
BASE COUNT         3042 a         2595 c         2978 g         3257 t
ORIGIN      
        1 tcttttttat catcaaatct acaacatgta actttcgaat gtaaagaaat tgagaattgc
       61 aattttacta aggcaacagt tgataatgtc atatttaaat gtcgacgttt acacaatgtg
      121 atttttctca aaacgagtgg cgaatgtgtt gattttagcc aaaatattct tgatacagtt
      181 gacttctcgc agagtcaact tggtcatagt aattttcgcg aatgtcagat tagaaattca
      241 aacttcgata attgttatct ttacgcttcg cacttcacca gagcagagtt tctgtctgcc
      301 aaagaaatat catttattaa atcgaatttg acagctgtta tgtttgatca tgtgcgaatg
      361 tcgacaggga atttttaaga ttgcattaca gaacaattgg aattaactat tgattattca
      421 gatatatttg ggaatgaaga tctcgatggt tatatcaata acattataaa aatgattgat
      481 acattgccag ataatgcaat gatattgaaa tccgttctgg cagtaaaact ggtgatgcaa
      541 ttaaaaattc tcaatattgt taataaaaac tttattgaga atatgaagaa aacatttagc
      601 cattgtcctt atataaaaga tccaattata cgtagttata tacattctgg ggaagatgac
      661 aagttcgata attttatgcg tcaacatcga ttcagtaagg tgaatttcga tacccaacag
      721 atgatccatt ttattaacag atttaatatg aataaagggc tggttgataa aaataacaat
      781 ttttttatcc aacttatcga tcaggcctta cgatcaacgg atgatatgat caaagcaaat
      841 gcctggtatc tttataaaga gtggattcgt agtgatgatg tttcacctat atttatagaa
      901 actgaagaaa agttaagaac ctttaacact aataaattaa cacgaaacga taatatattt
      961 atcctgttct cctctgtcga tgatgggcct gttatggtgg taagctccca gcgcttacat
     1021 gatatgttga atcctacaaa agataccaat tggaattcca cgtgtatcta taaatccaga
     1081 cataagatgt tgcctattaa tcttactcag gaaacacttt tcagctccaa atctcatggt
     1141 aaatatgcgc ttttcccaat ttttactgcg agttggcgag ctactcgtat aaagaataaa
     1201 ggtatttaag taaaggaaaa catcaccgtt cctagcatca tgggcggtga tgcccctatg
     1261 gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc
     1321 atacgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac atctcccgta
     1381 aacacttttc tgcgatacaa tgcctttaag ttatgtaacg gagagttcgg catgtcacac
     1441 cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag agatccctgc cctggatccg
     1501 caactgctcg actggctgtt gctggaggat tccatgacaa aacgttttga acagcaggga
     1561 aaaacggtga gtgtgacgat gatccgcgag gggtttgtcg agcagaatga aatccccgaa
     1621 gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc
     1681 gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tatcaacatt aagcgggccg
     1741 gaactagcgt tacaaaaatt gggtaaaacg ccgttaggac gctatctgtt cacatcatcg
     1801 acactaaccc gggattttat cgagataggc cgtgatgccg gactgtgggg gcgacgttcc
     1861 cgcctgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg
     1921 ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt
     1981 catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta
     2041 tgggcgttgt gggtggcgac accgggcgtt cctcagctct ggatcctggc ggtgtttgtc
     2101 gcgggtgtct ggctgatgcg cgctgctggt tgcgtggtga atgattatgc tgatcgcaag
     2161 tttgatggtc atgttaagcg cacggcgaac cgaccacttc ccagcggcgc ggtaacagag
     2221 aaagaggcgc gcgcgctgtt tgtagtgctg gtactgattt cgtttttact ggttctgacg
     2281 ctgaatacga tgaccattct gttgtcgatt gccgctctgg cattggcgtg ggtgtacccg
     2341 tttatgaagc ggtataccca tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg
     2401 attccaatgg cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc
     2461 ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcgatggt tgaccgcgat
     2521 gatgatgtga agattggcat taaatccacg gcaatcctgt tcggccaata cgataaattg
     2581 attattggta ttttgcagat tggcgtactg gcactgatgg cgatcatcgg tgagttaaat
     2641 ggcttaggct ggggatatta ctggtcaatt gtggtggctg gcgcgctgtt tgtttatcaa
     2701 caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac
     2761 tatgttggtc tggtactgtt tttaggactg gcaatgagtt actggcattt ctgatgatgt
     2821 aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgctg cgcttctcag
     2881 gcctacccgg tgaatcgcaa tttattgaat ttgcaggtca tggtaggccg gataaggcgt
     2941 tttcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg
     3001 tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc atcgtttctg
     3061 ctggttcggc atcgccgcta tcgctgatat acccttcatc acgcagcgtc agcaccagag
     3121 aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc acggagagac
     3181 gttgcgcaac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac gggttggcac
     3241 tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca
     3301 gctgtagggt gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca
     3361 gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac tcgtcgcgat
     3421 cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca
     3481 atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca
     3541 ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgtggca
     3601 gaatgatgat gtcgccgata gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt
     3661 gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag taaggtacgt
     3721 tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc
     3781 gctgacgtga tgccagtagc gcagtacagc acaggttcat ggcgtttgcc gcgcctgcgt
     3841 tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg
     3901 gacgcaccgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt
     3961 aggtcatcaa cggcattggc tcaccgaagt tgacgtaacc ctgaccgaga ttacgtagct
     4021 tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt
     4081 ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc ggaatcagcg
     4141 taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt
     4201 tcggatccag caaacgcccc gtgcgggaac gaccgccttc cacgaagtac tcgacggaat
     4261 aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaataa agtttattgc
     4321 ctttaaaagt acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc
     4381 agaagttcag gttgatcccg gcggcgatat gcggcggcac cagcccctgg tggtacagca
     4441 cgtaagaaag tagcaggtag tccatgtgac tgcggtggca aggcacatac accagctcat
     4501 gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg ccctgataaa
     4561 atcggttcca ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg taagagaaat
     4621 tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt
     4681 ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg agcagcttat
     4741 taaacagatc ctgacgagca ggcagacgtg ggcctacggc agccagacgt tgacgggcaa
     4801 agtgcatacg cgccacgcgc gccagtttct gagcgatagt tttatccgtg ccgtgttcat
     4861 ccgccatacg gcgcagcgac actgacggcg agaaacgcac aaaactgtcg cgaccgagcc
     4921 acagtacagc gaaaaatttc tgtacgccgt taagcatacg cagcggcggg ttcacttcgc
     4981 ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc tgcacatcca
     5041 gatttgggtt gctacggtgc aaatcgagat agtcgtggaa aagcttaata gactcttctt
     5101 tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat gaacacatag cgcggcagta
     5161 gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa atcatgtgcc agacactggg
     5221 cgcgcaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata attggacgag
     5281 aggtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga
     5341 tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg
     5401 taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt
     5461 ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg
     5521 gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact
     5581 ggattcaccc gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg
     5641 atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc
     5701 tgctggctgg atgtggacgc aattacccgc gtgctgctta tcagctccgt gatgctggtg
     5761 atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa
     5821 taccatgagc tttccggacg cgcaaaagat atgggatccg ccgcggtgct gattgccatt
     5881 atcgtcgccg tgattacctg gtgcatcctg ttatggtcgc attttggata acccttccag
     5941 atttcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc ttttgctgta
     6001 tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag
     6061 gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac
     6121 gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct
     6181 gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg
     6241 tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc
     6301 gcttctggcg caacagcata ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc
     6361 gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga
     6421 tggcgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc
     6481 acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggcaata aagtcgaact
     6541 gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac
     6601 cattgaaggg ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga
     6661 gaccgcgatg ccgcctggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg
     6721 catggcattc ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt
     6781 ctccaatatc accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga
     6841 tagtccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat
     6901 gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa
     6961 aaatcctcag gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt tgggggccgg
     7021 ggcgttaatt gcgctgttgc gtacgccgat tatcgatctg gcgctgcata ttgttggcgg
     7081 tagtgaagcg gtcctggaac aggcgcggcg ctttcttgaa atccgctggt taagcgctcc
     7141 ggcgtcgctg gcgaatctgg tattactcgg ttggttactc ggcgtgcaat atgcccgtgc
     7201 gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctggt
     7261 gatggggctg catatgaacg tgcagggcgc ggcgctggcg acggttattg cggaatatgc
     7321 aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg gaatttccgg
     7381 cgaaatgctg aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga
     7441 tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg tacttggtgc
     7501 gcgactgggg agtgacatta tcgctgttaa cgcggttctg atgacgctac tcacctttac
     7561 cgcctatgcg cttgatggtt ttgcctacgc ggttgaagcg cactccggtc aggcatacgg
     7621 tgcgcgcgac ggtagccagt tgctggatgt atggcgggca gcgtgccgcc agtcggggat
     7681 tgtagcgtta ctgttttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct
     7741 gacgtcgtta acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt
     7801 gccgttggtt ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc
     7861 cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga cgctccttac
     7921 gctgccgtgg ctgggtaatc atggtttgtg gctggcatta accgtctttc tggcgttgcg
     7981 cgggctttct ctggcggcta tctggcggcg tcactggcgc aacggtacct ggtttgccgc
     8041 aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc aaattgctga ctacacttaa
     8101 tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga
     8161 ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc
     8221 aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt gatcaactgg
     8281 tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg
     8341 attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga cgtgtacaag
     8401 gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca tgctggcact
     8461 gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca tgtgcttcaa
     8521 tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcata atgtcttcca
     8581 cgccttctgc acactcttct ttcaccgcgc cgcagcgatc gcaaataaac atggctgacg
     8641 tatgcgtggg ctggtcgaac agatgacaga gcacataact gttggtggat tccaccttat
     8701 gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc ggcttggctt
     8761 gcggttcagc ttcgcgcagt aaatcaagca gatcataagc gctgatagcg ccatcctgca
     8821 gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc
     8881 agattttttc agcctgcgct aataactcct gcgttgtggt cttttccatc gggcacctca
     8941 aagtgggggt taagaatgtc cttactttac catgttccag gaaaaacacc gagaaccctt
     9001 attgttgccg taatgttgat tttctgtttt gtaggtaagg tgttgtgttg ccttgtcgta
     9061 ccattatcaa cacgataata attaataatt catttttaaa taaattgctt caagttaact
     9121 cgctatttat ttaactgggt ttaaacgcat acctgtagtt cagaaattat gagcgatttt
     9181 tgatagtgac cagatggcaa aagaattata aggaggacac ggtacgacaa aaaaggtaga
     9241 tgaatcatgt tgaagataat acccggtgca actggatact ttaataaaac actcaattca
     9301 aatcaattcg aaaataaaga tgcaaccaaa gataaattgg ataatagagg ttcaatcaaa
     9361 ggtaaattaa ataatatata tggtaaatct attgattatg cagcgctgcg tcatcgggat
     9421 ataattattg caaaaataga tttgtttatt caacgaatca cgcataattt atggcatgca
     9481 cgtaaaaaaa tgtgttttta attgagcaaa taaatgatct caaaatgtgg gtcaataagt
     9541 atactgacga ttgcactgat gaggatttaa acgatcgtga ctttattgca tcagttgttg
     9601 accgggctat ttttcatttc gcgattaata gtatatgtaa tcctggggat aataaagatg
     9661 caatgcccat tgaacaatgt acttttgatg tagaaactaa gaatggcctt ccctccacgg
     9721 ttcagctatt ttatgaggaa tctaaggata atgaaccttt agcgaatata cattttcaag
     9781 caataggttc tggtttttta acgtttgtta atgcctgcca ggaacatgat gacaacagct
     9841 taaaattatt tgcttcgctg ttaatttcac tttcatattc tagtgcctac gcagatttat
     9901 cagaaacagt gtatattaat gaaaataatg agagctacct gaaagctcag tttgaaaaat
     9961 tatatcaacg tgatatgaag aagtacctgg gagagatgaa gcgtctggca gatgggggag
    10021 aaatgaattt tgatggctat ctggataaga tgtcacatct ggtgaatgaa ggaacgctcg
    10081 atcctgatat tttaagcaaa atgcgagatg ctgcaccaca attaattagc ttcgcgaagt
    10141 cgtttgaccc aacctcaaag gaagagatta aaatacttac agacacttct aaattaattt
    10201 atgacttgtt cggggttaaa tcggagaaat aatatgtgaa gttcttcgat agtatggaag
    10261 gccttatata aaaggaccca atatttattg ggttcttttt tctctatcaa tgctattagc
    10321 agggagatat atcaccagag tttaatgtgt gattttttat ttatcgtcga acatggattg
    10381 tttatcattg gccttaacaa agttaacggc taataagatt atttccatca cttcgtgaga
    10441 gcttcatgcc ttgagaggat ctcaattttc ttttgcaatg agacaggcgc ttcctgttgt
    10501 tatggtatag taccccgcta ttgagcctcc tgaacagtga agctgaataa cataacccca
    10561 tgatatatcg ataaaataat ctctacattt gaaaatgcac ggtaattctg aaatgcaaaa
    10621 aatcaaccaa accagcgcaa tgcctgaaaa aactgacgtt cactggagtg gtcggtttag
    10681 cgttgcacca atgctcgata ggatgtaccg tttttgaaaa acaactagtt atacactttg
    10741 tgggagccta ttgggaacct gttattctta gaaagcatca tttgctcatc acttcaattg
    10801 accatggtta aagccagcta taaacttgac aaacgaggta actggttgct taggcccaag
    10861 tgggcacacc ataagaagct catgttggtg atgaagcaac ccatgaagta tcgagaataa
    10921 ttataaagtt cactatcttt gaggtttctt atatgttttg cgaagaaaaa gtagctcaaa
    10981 tggctgcata cctacttctt aagagaggtg ggcgcatggc atatctgaaa ttgatgaagc
    11041 tgctctatct gtctaaccgc cagtcgattt tgaagcatgg caggatgatc ggcgaagata
    11101 gcctttactc tatgaaattt ggaccagtca tgtcgaatac gctgaacttg attcgcggta
    11161 aggctgaagg cattggtgac tactggtaca acttgataga gacgaacggg cataatgtat
    11221 cgttgcgttc agatccgagg gaaatggatg cagacgaggt ctttgatgaa ttgagtcgtg
    11281 cagatatccg gattttagat gaaatctatt ctcggtatgg gcatatgaac cgatttgatc
    11341 tcgcaaatat gacgcattta gaaagcgttt gtccagagtg gcacgatcct ggcaattctc
    11401 gtaagcctat agacctgaaa gaaatgctga tcagtgaggg taaaagcgag gatgaggcta
    11461 atcgcataat tggcaaaatg gaagaatctc agaaacttaa ggaattttct ttgcaattat
    11521 catgacggat tatcagccat acaggaaagg aactgtgctt gccccaactg gaccatgcaa
    11581 tcatcttcat gtgatttgta atgatcctgt ttattacccc gttaacgatt gttattgtgt
    11641 tttagttgtt aatatttcta gtatcaagga tggtgtcccc cacgatccgt cttgcgtctt
    11701 gaattctggt gatcatcgct ttatcaagca tccaagttat gttgtttacg ctgaagctat
    11761 aatttggcga gtggataaca tggttagaaa gcagcgatcg ggtgagattt ctgttcatga
    11821 tgatatgcca gaagctacat tcaatagaat tctggacggt tttgatatct ct
//
LOCUS       DADLSB010000114        11862 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.114, whole genome shotgun sequence.
ACCESSION   DADLSB010000114 DADLSB010000000
VERSION     DADLSB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.114"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            252..699
                     /locus_tag="JN331_002991"
                     /pseudo
     CDS             252..699
                     /locus_tag="JN331_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134906.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            699..1691
                     /gene="lsrD"
                     /locus_tag="JN331_002992"
     CDS             699..1691
                     /gene="lsrD"
                     /locus_tag="JN331_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HAY7261084.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTATAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            1703..2725
                     /gene="lsrB"
                     /locus_tag="JN331_002993"
     CDS             1703..2725
                     /gene="lsrB"
                     /locus_tag="JN331_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HAY7261085.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYGERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            2752..3627
                     /gene="lsrF"
                     /locus_tag="JN331_002994"
     CDS             2752..3627
                     /gene="lsrF"
                     /locus_tag="JN331_002994"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY7261086.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3651..3941
                     /gene="lsrG"
                     /locus_tag="JN331_002995"
     CDS             3651..3941
                     /gene="lsrG"
                     /locus_tag="JN331_002995"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY7261087.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3980..4738
                     /gene="tam"
                     /locus_tag="JN331_002996"
     CDS             3980..4738
                     /gene="tam"
                     /locus_tag="JN331_002996"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY7261088.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4742..5656)
                     /locus_tag="JN331_002997"
     CDS             complement(4742..5656)
                     /locus_tag="JN331_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family inner membrane protein"
                     /protein_id="HAY7261089.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(5863..7314)
                     /locus_tag="JN331_002998"
     CDS             complement(5863..7314)
                     /locus_tag="JN331_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY7261090.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLVGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(7541..8959)
                     /locus_tag="JN331_002999"
     CDS             complement(7541..8959)
                     /locus_tag="JN331_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7261091.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(9098..9457)
                     /locus_tag="JN331_003000"
     CDS             complement(9098..9457)
                     /locus_tag="JN331_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY7261092.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(9457..10383)
                     /gene="glsB"
                     /locus_tag="JN331_003001"
     CDS             complement(9457..10383)
                     /gene="glsB"
                     /locus_tag="JN331_003001"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY7261093.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(10447..11406)
                     /locus_tag="JN331_003002"
     CDS             complement(10447..11406)
                     /locus_tag="JN331_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7261094.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAAIGAQAGAALKKCVLELGGSDPFIVLN
                     DADLELAVKAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDE
                     ENALGPMARFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTA
                     FREEMFGPVAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFI
                     NGYCASDARVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            11486..>11862
                     /gene="yneK"
                     /locus_tag="JN331_003003"
     CDS             11486..>11862
                     /gene="yneK"
                     /locus_tag="JN331_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7261095.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEIN"
BASE COUNT         3076 a         2973 c         2937 g         2876 t
ORIGIN      
        1 gacagacact gcgctcttag catggggcag ggaataacca ggctatttct ccatgcacag
       61 gccaattatg aaagaggaga ggcttaagtt cttgaaccct gaacgaataa cgactaccag
      121 aaaagcactt cgcatcactc agacatgatt taacataata tacattatgc gcagtaacat
      181 tgtagtagct aaattgagat gtccgtctct gactaaatag acatgtccgc gctacagtct
      241 caccacagcg attgctgctt ggcatttcag caattggttg gttgacgata attctggtgg
      301 catttatggc ctggctgcat ggcggcactg gcgggaattg tgtttgcttc gcagattggt
      361 tttatcccca accagaccgg taccgggctg gagatgaaag caattgcagc ctgcgtgctg
      421 ggcggcatta gtttgctcgg tggttccggt gcgatcatcg gtgcggtact cggcgcatgg
      481 ttcctgacgc agatcgatag cgtactggtg ctgttgcgca ttccggcatg gtggaatgat
      541 tttatcgcgg gtctggttct gctggcggtg ctggtgtttg atggacgcct gcgttgtgcg
      601 ctggaacgta atctacggcg gcaaaaatat gcccgcttta tgacgccacc gccatccgtt
      661 aaacccgctt cgtcaggtaa aaaacgggag gccgcataat gcgtattcgc tacggttggg
      721 aactggctct tgccgcactg ctcgttattg agattgtcgc atttggtgca attaacccgc
      781 gaatgttaga tctcaatatg ttgctgttca gcaccagtga ctttatctgc attggcattg
      841 tcgccctacc gctgacgatg gtgattgtca gtggcgggat cgatatttcg tttggttcga
      901 ccatcggcct ctgcgccatt gcattgggcg tactgtttca aagtggtgtg ccgatgccgc
      961 tggcgatact cctgacctta ctgctcggcg cattgtgcgg gctgatcaac gccggattaa
     1021 ttatctatac caaagttaac ccgctggtga ttacgcttgg cacgctgtat ctgtttgccg
     1081 gaagcgctct gctgctttcc ggtatggccg gagcgacggg gtacgaaggt attggtggat
     1141 tcccgatggc gtttacagat ttcgctaacc tggatgtgct gggactcccc gttccgctga
     1201 ttatcttcct gatatgtctc ctcgttttct ggctctggct gcataaaacc catgccggac
     1261 gtaatgtgtt tttgattggg caaagcccgc gcgtggcgct ttatagcgcg attccagtta
     1321 accgcacctt atgtgcgctc tatgccatga cggggctggc gtctgcggtc gccgctgtgc
     1381 tgctggtatc gtattttggt tcagcacgtt ccgatctcgg tgcgtcgttt ctgatgcccg
     1441 ccatcaccgc agtggtgctt ggcggggcca atatttatgg tggttccggt tccattatcg
     1501 gcaccgccac tgcggtttta ttagtgggat atttgcagca aggtttgcaa atggcaggag
     1561 tgccaaatca ggtgtccagc gccctttccg gtgcgctgct tatcgtcgtt gtcgtaggtc
     1621 gttccgttag cctgcatcgc caacaaatta aagagtggct ggcgcgtcgg gccaataacc
     1681 cattgccata aaggatatct tcatgacact tcatcgcttt aagaaaatcg ccttactgag
     1741 cgctcttggc attgccgcaa tctctatgaa tgtgcaggcc gcagagcgta ttgcatttat
     1801 tcccaaactg gttggcgtgg gattttttac cagcggtggc aacggcgcac aacaagcggg
     1861 taaagagctg agcgttgatg tgacctacga cgggccgaca gaacccagtg tttctggtca
     1921 ggtacagttg attaataact tcgtcaatca aggttataac gccattatcg tttctgcggt
     1981 ttcgcctgat ggcttgtgtc cggcactgaa acgcgccatg caacgtggtg tgagagtgct
     2041 gacctgggac tctgatacta aaccggagtg ccgctcttac tacattaatc agggaacgcc
     2101 cgcccagttg ggaggtatgt tggtggatat ggcggcgcgt caggtgaata aagacaaagc
     2161 caaagtcgcg tttttctact caagccccac cgttacggac caaaaccagt gggtgaaaga
     2221 agcgaaagcg aaaatcgcca aagagcatcc aggctgggaa attgtcacta cgcagtttgg
     2281 ctataacgat gccactaaat cgttacaaac cgcagaagga atattaaaag cgtatagcga
     2341 tctcgacgcc attatcgccc ccgatgccaa cgccctgccc gctgccgcac aagccgcaga
     2401 aaacctgaaa aatgacaaag tagcgattgt cggattcagt acgccaaatg tgatgcgccc
     2461 gtatggagag cgcggcacgg tgaaagaatt tggactgtgg gatgtggttc agcaaggcaa
     2521 aatttcagtg tatgtcgcgg atgcattatt gaaaaaagga tcaatgaaaa cgggcgacaa
     2581 gctggatatc cagggcgtag gtcaggttga agtctcgcca aacagcgttc agggctatga
     2641 ctacgaagcg gatggtaatg gcatcgtact gttaccggag cgcgtgatat tcaacaaaga
     2701 gaatatcggc aaatacgatt tctgatgtgc ataacttaac cggagtaagt tatggcagat
     2761 ttagacgata ttaaagatgg taaagatttt cgtaccgatc aaccgcaaca aaatatccct
     2821 tttaccctga aaggttgcgg tgcgctggat tggggaatgc agtcacgctt atcgtggata
     2881 tttaatccga aaacgggtaa aactgtgatg ctggcctttg accatggtta ttttcagggg
     2941 ccgactaccg gacttgaacg cattgatata aatatcgccc cgctgtttga acatgccgat
     3001 gtattaatgt gtacgcgcgg cattttgcgc agcgtagttc ccccggcgac caataagccg
     3061 gtggtactgc gggcgtcagg tgcgaactct attctggcgg aattaagtaa tgaagccgtg
     3121 gcgttatcga tggatgatgc cgtgcgcctg aacagttgcg cggtggcggc gcaggtttat
     3181 atcggcagcg aatatgaaca tcagtcgatc aaaaatatta ttcagctggt cgatgccgga
     3241 atgaaagtgg gaatgccgac catggccgtg actggcgtgg gcaaagatat ggtgcgcgat
     3301 cagcgttatt tctcgctcgc gactcgaatc gccgctgaaa tgggggcgca aattatcaaa
     3361 acctattatg tcgaaaaagg ttttgaacgg attgttgccg gatgtccggt acccattgtt
     3421 attgctggcg gtaaaaaatt accggagcgc gaggcgctgg aaatgtgctg gcaggctatc
     3481 gatcagggcg cttctggtgt ggatatgggg cgtaatattt tccagtctga ccatccggtg
     3541 gcgatgatga aagccgtaca ggcggtggtt caccataacg aaacggctga tcgggcatat
     3601 gaactctatc tgagtgaaaa acagtaactg cggatttaag gagaagaatt atgcacgtca
     3661 cactggttga aattaacgtt catgaagaca aggttgacga gtttatcgaa gtttttcgcc
     3721 agaaccacct gggctctgta caggaagaag gtaatttgcg cttcgatgtc ttacaggacc
     3781 cggaagtgaa ttcgcgcttt tatatttacg aagcctataa agatgaagac acagtggcat
     3841 tccataaaac cacgcctcac tacaaaacct gtgtcgcgaa actggaatct ttaatgaccg
     3901 ggccgcgtaa aaaacgtctg ttcaatgggt tgatgccgtg aggctacaat tggggtaacg
     3961 cgctgacggg agtaaaaaaa tgtctgactg gaacccctct ttatatctgc acttttccgc
     4021 tgaacgatcg cgtccggcgg tggagctgct tgccagagtg ccgctggaga atgtcgaata
     4081 tgttgccgat cttggctgtg gtccaggtaa cagcaccgcc ctgctgcatc aacgttggcc
     4141 tgcggccagg ataacaggca tcgactcgtc tccggcgatg attgctgaag cgcgcagtgc
     4201 tttgccagac tgccagttcg tggaagcaga catccgcaac tggcaaccgg aacaggcact
     4261 cgatctgatt tttgctaatg cctcactgca atggctgccc gaccactacg aattgtttcc
     4321 tcacttggtt tctttactta atccgcaggg cgtgctggca gtacagatgc cagataactg
     4381 gctggagccg acccatgtgc tcatgcgcga agttgcctgg gaacaaaact acccggatcg
     4441 cgggcgtgag ccgttggctg gcgttcatgc ttactacgat attttgagcg aagccggatg
     4501 tgaggtcgat atctggcgaa ccacttacta tcaccagatg ccgtcgcacc aggcgattat
     4561 cgattgggtg accgccactg gattacgtcc gtggttacag gatctgaccg agagcgaaca
     4621 gcagcttttt cttacgcgct accatcagat gctggaagag cagtatccac tgcaagagaa
     4681 cggacagata ctgctggcat ttccgcgtct gtttattgtt gcccgccgta cggagtaatt
     4741 atcacgtcag ctggtaatga cgatcgggaa gaatttttgc tggaatttct gcttcatcgt
     4801 tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc atccagcggt aaatcattgt
     4861 tttcagtacc gaacggatct tccagttctt ccgccagaca atccagcgaa ataaaagtgt
     4921 aggaaatcag cacagagata aaaggcgtca tgtaatgcag gtccacgacc agcgcgaacg
     4981 gcagcatgat acaaaacaga taaacggtac gatgcaaaat cagggtgtag gcaaagggaa
     5041 ttggcgtgta ggcaatgcgc tcacagcctg ccagaaccgc tgaaatatca ttaagccgat
     5101 cgttgaggct aataaacagg atatctgaaa gctgtccatt gcggcgctga accgccaacc
     5161 attctcccat tattaacaag atacggttag ctggagagtt cgaagccagt acacgctgaa
     5221 gatcttcagt cttgagataa tgagccagca cttccgcctg tggctgtttg cgtaatgtca
     5281 tgcgtaaaca gtgggcgaag gcgatttgca gcctggcaaa ctcccttaca cttgccgaat
     5341 ccggcaatgt cgtttttacc tctcgcagta acgaccgtga ggcaatcatc aactgccccc
     5401 aaagttttcg cgcttcaacg taacgggcgt acccggcatt attacgaaaa ccaagaaaaa
     5461 tggcgatggc gacaccgaga atgctgaacg gtgcgagggt gaatttgatg cccagatgcg
     5521 tgtaccaggg cagcatgaat ataacagcaa tagaaaagag aaaattgagt agtaagcgcg
     5581 aggatatctt ggataatact gagccgtgcc agacaaaaat acggcgcagc cagtgttgtt
     5641 gtggacgaac aatcatggtt atcttcaggc gtggaaaagt cgccctatta aacgtgatta
     5701 cgatcacatt ctcaagacgc tacttacaaa ttacctacaa gcttacaaca ttaacaggcg
     5761 ctgcatgtga cacctgttaa tgataaaggc atataggatg ttgtaactaa tatagtgaaa
     5821 taagaaaccc ggtcgaaacc gggttcagaa gtaacggtgt tattagcaca acggacgtac
     5881 agcttcgcgc atcccttttt caagaatcgc atccaggtca ttagcaacct gctcgaccag
     5941 accaggcact tgcgtcaggt cctgctccca gtggtctttt tctgccaaca caatcgctac
     6001 cagttcctgg gtaccgataa cacggtcacg atgctggctc cagagttgct gataacgttc
     6061 cagccagtgt gcatcgtcct gtaccggata tgtttctccg ttacgctcac cacgatagaa
     6121 cgcaatcaat gccgctaatg cgaaagtaag gcgcgccgga agtgtgccgt ttgccttctg
     6181 ccctaccagc agctgcggca ggatgcgggt gcggaacttg gtcataccgt tgagcgcgat
     6241 agacagcagc tgatgcttaa tgtacgggtt acggaaacgc ccggtgactg cactggcgaa
     6301 agattccagt tcatcacgag gcaaatccag taccggaata atttcttcgt agatggcttt
     6361 ttcaacgaat gcgcaaattt cagcatcgtt catcgcttca cctacggtat ccagccctgc
     6421 ctggaacgcc accggcacca gcgcggtgtg cgcaccgttg aggattgcca ctttgcgttc
     6481 tttatacggt ttaatatcgt caacaatcag cacgttgagc ggatatttgt ccaggcgcag
     6541 ctcagtcgct aaggatttcg gcccctgaat cacaaacagg taaaagtgtt cagcggtgtc
     6601 gaggaaacca tcgtgataac ccagttcttc ttccagttta gccacttcat cgcgcggata
     6661 accggtaacg atacggtcca ccagcgtaga acagaagctg ttagcctgat ccagccattg
     6721 aataaatgct tctggcagtg cccactcttg cgcatagcgc agcaccagtt cacgcaacgc
     6781 gtcgccattg tagtcaatca actcacacgg aatgatgatc caacctttat ccagcgcacc
     6841 gttgaaatgg ctaaagcgtt cgaacagcag acgggtcagt tttgccggat agcttaccgc
     6901 tggcgcgtca tcgaatttat cgcccgcatg gtagctgata cccgcttcgg tggtgttaga
     6961 gaaaacaaag cgcatttccg ggttgtgcgc cagtttcagg aattcatcgt attcactgta
     7021 gacgctgatt tcacgattaa ccgagcgaat cagacgcgcg tcgctgaccg cttccccctt
     7081 ctcattcagg ccacggataa tggtggtgta cagaccatcc tgcgtgctca gtgacggcgg
     7141 gaatgaagtt tcaatcggac gaacaacgac cacgccagaa ttcagatcgg tgtgctcatt
     7201 caggagatcg atttgccagt caacaaaggc gcgcaggaag ttaccttcac caaactgaat
     7261 gatacgttct ggatactgtg caccgggaaa atcgcgacga tttagtgttt tcacaatggg
     7321 ttcccttctg attagtcata caacctgttt gaattggtgc gacaggttag caaactttaa
     7381 tacgccgaac ccctgttttg atcaactcct gatgattaat gagcagtttt atgagaaaag
     7441 tgtggcgcga atcatggttt aatcgaggaa atacgccttt tcctggatca taaagtggta
     7501 gaacacattg cattcaaatc gcgcggaatg aataaagatg tcagacaact tcctcaccgt
     7561 aacgcatagt gctggtacgg ttgcgcccat ctttcttcga gcgatacaga cacgtatcgg
     7621 cttcgaccat caatttatta aaatcatcgg tcagcgttcg gtacgatgcg cgaccactac
     7681 cgacgccaat acttaccgtg agataaagcg ttttttgttg ccaggtgaat ggttgcagtt
     7741 caacgccttt acggattttt tccgccatta gcagaccatc gacaggattc accgacggca
     7801 ctgcaacagc aaattcttcg ccgcccatgc gcgccaccag ccccttatca ccgacaatct
     7861 tctgaatatg ccgggcaaac acgcttaaca ctttatcgcc acattcatgt ccatagttat
     7921 cgttaatgct tttgaaatag tcgatatcaa gcagcatgac cgtcagatgt tgtgtctgtt
     7981 tcagcgatgg acttttcagc gcctcataaa gaccggaccg tgagtaaacc tgagtcagaa
     8041 aatcaaagtc ggctcgcagc gcaacttgct tcattagcaa attgatcgct gccacgctaa
     8101 aagaaaccat aattgggcat atcgccatcg tggcaatacc gagacgtgcg gagaacattt
     8161 ccggaatgga gaacggcgaa ccgaccgaga tatcaatcac cgaatttgcc accagtacga
     8221 tttccaccgc accggtgaca aaggtcaaca gacatgttac ctgcggcgta tagcgcactg
     8281 cacaccagat taatgcaggc agcgggaacg ccagactccc cgctccgcca atgactaccg
     8341 aggcaataac cgacacaata agcgcgatgg cgggcatcat ctgttctgct ttaaagcgcg
     8401 gcagcactcc aggaatagcc aacgtcagca tacaaggcac gatcaacacg cccgttgaga
     8461 attgctcact gaaccagtcc gcaagcaaag gccagaaatc cagactatca atactgaccg
     8521 aaccaatcgc cccgacaata gcgcataata atgcggcaat cagacagtaa ttaaatagcc
     8581 gtaaggcact tactggctca tacttatttt tccccagacg cttatcccgc acgaccagta
     8641 aggcgacggt aacaataaac atcatattgg agaaattgat aaccagtgaa accagtcccc
     8701 attcggtggt tatggcatca taaaccagca tcgccacata actgatcgca tagtaatgca
     8761 gtcgattaag ccagacatag cgggcaaaca cccccgccat tacgccattc agcggccaga
     8821 aaagagaaag ttcgtgaacc agccgtaact ctgcaccaat aaaatagaac agcgtggtta
     8881 gcgcaaaaat agcgatgcta ttacgtagca gatgaccttc ctggaacaaa cggaaagttg
     8941 aaatggggtg tacatgcatt aattatctgt ccatacaatt gcgggtggca attatctcaa
     9001 cgtaataagt aaattctcat aaaggatagc atgttcgcta acagcggcgg caaataaagg
     9061 taccctggga atgctatttt atcgttttcc gcaattctca tggctgattc atctgtacaa
     9121 ccagccagtg atagattacc gccacgatat aacgttgttg ttcttcgctt aacgaaacac
     9181 cctgcggaat gttatgccac ttagccagac aaccgcgaca gcaggtcgcg gtggcgtgct
     9241 gagcgataaa caccggatgc ccgcgcatgg gggtttgctt accgtcatta gccggtaatg
     9301 ccggagcaag acgtttagcg acaaaatccg ccgcatgttg ttcgatgact ggcgcgcctt
     9361 tctccaggca atactgacgc tctttcatgc ccagacgaaa gcgagagcga aattttgaac
     9421 gggataaacg cgcgaagagt ggatcgagag actgcattaa taaaccgaac gccctaactg
     9481 tttcgtcaat tgttcaagga cggcgatacc cgcaagcgag ttacctgcat catccagttc
     9541 cggactccag actgcgatgg ccatttcatg cggaacaatc gccacaatac cgccaccaac
     9601 gccagatttc gccggtagtc ccacccgcca ggcaaactcc cccgcgttct ggtacatacc
     9661 actggtcgcc atcagcgcgt taatttgccg cgcctgcatt ggcgtcacca caggttcatc
     9721 aatatgaata gctttcccct gattagccag aaagacaaac gtccgggcca gctctacaca
     9781 gctcattttc agagcgcagt aatgaaagta gttttgcaga acggttgtca cgtcatgatg
     9841 gaaattgcca aacgacttca tcagccaggc gatagccgca tttcgcgcgg aatgttcaaa
     9901 ttcggaacgc gctaccaccg tatcgtatga aatatcagac acaccgctta agccgcgcac
     9961 gacttccagc atacgttgcc gtggtgcgct tagtcgccct tgcaacatat cgcagaccac
    10021 cagcgcaccg gcattaatga acggattacg cggaataccc tgttccattt ccagttgcac
    10081 taaagaattg aacggtgatc cagacggatc tttgccgacg cgttgccaga tttcctcttc
    10141 ggagtaatga cgcatggcga caacgagact cagcactttg gaaatagact gaatggaaaa
    10201 acgttcttgc gcgtctccgg cctgaaaaag ctgtccgtca acggtacaga tagcaatccc
    10261 caatcgggaa ccgtctactg tagccagcgc cggaatataa tccgcgactt taccctgacc
    10321 aatgagcggc cgcacttgcc gcaagatgtt ttctaaaatt gcattatcca tggcgactgc
    10381 cactttctac tcctggaccg caggtctgaa aagacctgcg agtatatcag agctgaatat
    10441 gtcgcgtcag atccggtctt tccacactgt ctggatatta cagaattcgt gtaagccgaa
    10501 atgggaaagc tcacgaccaa agccactctt tttcacgcca ccaaaggcca ctcgcgcgtc
    10561 gctggcacaa taaccattga taaacacccc accgcattcc agacgtgccg ccatctgtct
    10621 ggcctgtgtt tcgtcggtgg taaaaatggt cgctgaaagg ccgaactcac tatcattagc
    10681 cagttccagc gcatgttctg catctttcgc aatggtgatt gcagcaacgg ggccaaacat
    10741 ttcttcccga aacgcggtca tttctggggt aacattcgcc agaaccgttg gcggatagta
    10801 gttacctgcc ccagccatct tttccccgcc cagtaacaaa cgcgcaccct gagccagggt
    10861 tttctccacc tgatgatgca gctcatcacg taaatcaaaa cgagccattg gtccgagagc
    10921 gttctcttca tcacggggat cgcccatttt caaggctgcc gcagctgaca caaaacgttc
    10981 ggtaaatgcc gaagcaattc cctcttcgat aataaagcgt tttgccgctg cacaaacctg
    11041 tccggtattc tgataacgtc cggctaccgc cgctttaacc gccagttcca gatcggcatc
    11101 gttaagcaca ataaatggat ccgaaccgcc cagttccagt acgcattttt tcagtgccgc
    11161 tccagcctgt gcgccaatag ccgctccagc acgaacactt ccggtcaccg tgacagcagc
    11221 aatgcgcgag tctttaatca tctgactgac accgtcgttg tcggcattca gccagccata
    11281 tacgccttgc gggattcccg catctttaaa cacttgggca atgagctgag cacagcccat
    11341 cacattcggc gcatgtttaa gtaagtagcc gttacctgca agaatgatgg gaacagcgcc
    11401 acgcatcacc tgcctttgat agttcttgca gctcaaatgc cctatatagt gatgttccga
    11461 tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca attacatatc
    11521 tcttcctgat gattttcccg ctcgtaatat tgcacctcaa gtaaaagaag ttttaaagga
    11581 ttttattgat gcactcagta ccataatatg tgatgaagag tggcgcacgt ctttaaacac
    11641 caactcagcc acaaaaaaga tatttaacaa ccttgataac ttatcatata ttcagaggac
    11701 ttcttttcgg ggtaacgaca cactatacaa tgaaaaggtt cagtttaaac tcacttatcc
    11761 cgtcaagaat ggaagacaca aagaaaatat tgaatttcag gtagtaataa atttaagccc
    11821 tatttattta gataattttc gccatgatgg agaaattaat at
//
LOCUS       DADLSB010000115        11755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.115, whole genome shotgun sequence.
ACCESSION   DADLSB010000115 DADLSB010000000
VERSION     DADLSB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.115"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(137..1501)
                     /gene="tdcG"
                     /locus_tag="JN331_003004"
     CDS             complement(137..1501)
                     /gene="tdcG"
                     /locus_tag="JN331_003004"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7261096.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRNKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1573..1962)
                     /locus_tag="JN331_003005"
     CDS             complement(1573..1962)
                     /locus_tag="JN331_003005"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY7261097.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1976..4270)
                     /gene="tdcE"
                     /locus_tag="JN331_003006"
     CDS             complement(1976..4270)
                     /gene="tdcE"
                     /locus_tag="JN331_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAY7261098.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4304..5512)
                     /gene="tdcD"
                     /locus_tag="JN331_003007"
     CDS             complement(4304..5512)
                     /gene="tdcD"
                     /locus_tag="JN331_003007"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY7261099.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5538..6869)
                     /gene="tdcC"
                     /locus_tag="JN331_003008"
     CDS             complement(5538..6869)
                     /gene="tdcC"
                     /locus_tag="JN331_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY7261100.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6891..7880)
                     /gene="tdcB"
                     /locus_tag="JN331_003009"
     CDS             complement(6891..7880)
                     /gene="tdcB"
                     /locus_tag="JN331_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY7261101.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSLLIDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7979..8917)
                     /gene="tdcA"
                     /locus_tag="JN331_003010"
     CDS             complement(7979..8917)
                     /gene="tdcA"
                     /locus_tag="JN331_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY7261102.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9107..9451
                     /gene="tdcR"
                     /locus_tag="JN331_003011"
     CDS             9107..9451
                     /gene="tdcR"
                     /locus_tag="JN331_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY7261103.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9707..10247
                     /locus_tag="JN331_003012"
                     /pseudo
     CDS             9707..10247
                     /locus_tag="JN331_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10269..11435
                     /locus_tag="JN331_003013"
     CDS             10269..11435
                     /locus_tag="JN331_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HAY7261104.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIKRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPV
                     LISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYTMD
                     LNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         3335 a         2839 c         2548 g         3033 t
ORIGIN      
        1 aagactttaa aataacggag atggataaga attttctact aaattaatcg caagagaata
       61 ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca cccaaggatg
      121 aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg cgtgatgttt
      181 cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc actttatcga
      241 gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt actgctttca
      301 cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca accggatcgc
      361 aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc tgcgccggac
      421 tgccgcccag tagttcagtt aaccctgccg ccgccattga acaggccacg ccaatctccc
      481 cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc gcgccaatag
      541 cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga cggaacttat
      601 cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt gccgttacca
      661 cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg atccagtcga
      721 tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga cgcagcgcta
      781 cggcacgacg cggcacattg agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt
      841 caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt tccgctttgt
      901 tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg ttgtaatcac
      961 acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt
     1021 gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag taataggttt
     1081 tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc tcatggcgag
     1141 gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca atatgcgcac
     1201 cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca gggatctcat
     1261 caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg acatccgtgg
     1321 catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata tggctcgtcg
     1381 cggttaataa gccgctactt tccagccgat caataaaact ttttccggca ttcattggcc
     1441 ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat gcactaatca
     1501 tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg cgaccgtccc
     1561 gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct tcacatcttt
     1621 cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa
     1681 gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga tgaacacggt
     1741 catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt tgacgttttc
     1801 gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct gcgggcaaac
     1861 cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa cataagggcc
     1921 gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc cggcgtcaga
     1981 gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc agtgcgttga
     2041 agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc gggtgctcga
     2101 tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga ccgccttcga
     2161 catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt ttacgtactg
     2221 gatcttcttt gcccagcgcc gcaggaacga ttgagaaagt gtacgagatc ccatctttgg
     2281 cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct ttgcggtcac
     2341 gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg
     2401 tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc gactgggtag
     2461 ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt tcaaccaggt
     2521 cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga
     2581 tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg gcatatttga
     2641 tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat
     2701 agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt
     2761 agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg aagtgatcga
     2821 ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct gttttcggcc
     2881 cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg
     2941 ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt gggctgacgc
     3001 agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg tcattttcat
     3061 actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag gcaatcggta
     3121 attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata gtgtgcaggg
     3181 tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc cccatcccgc
     3241 cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat tccggtgtac
     3301 gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc tgtgcctgct
     3361 gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg aacgatgccg
     3421 tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga taagcgaagt
     3481 agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca aagccatatt
     3541 tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc tcctcacgca
     3601 gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga tcggcaaatt
     3661 gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg cgatagtcac
     3721 cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca gatttacggc
     3781 agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt ttacgcagat
     3841 cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg aatgaacttt
     3901 taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca tccgtttgca
     3961 ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg gtaattgtgg
     4021 tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata cggatgcctt
     4081 ccattacttt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga aaagattcat
     4141 cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt tcgcttttcc
     4201 agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg gtatcaatat
     4261 ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca aattctgcgg
     4321 gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct tcgttagtcg
     4381 gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg ccaaaagagt
     4441 tagatcgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa tgttccatga
     4501 ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata attccatcca
     4561 gccgacgtaa tgaagctgcg tgtccggcaa tatgacgggc aattcggtga acaaaggttt
     4621 taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga acacgtaaat
     4681 ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg cgttccaggt
     4741 cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag tcgacatcgc
     4801 cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag gtgtcaacac
     4861 tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc acaaccaggc
     4921 cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg
     4981 acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc cacggcaggc
     5041 cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat accgccacct
     5101 gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt gcgtaattat
     5161 gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg gtaataatgg
     5221 cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag gccacgctgt
     5281 catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct tcgtagctgt
     5341 ggagagccag cggtgctggc tctcccccat ttacggataa gaatgcattt tccgagttaa
     5401 taccgtcggc aatacctgac attaatactt cacagtcgct ggcatcgagt acggaaaact
     5461 taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc atctcttttc
     5521 tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt cagcagacca
     5581 atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga cggcgcttta
     5641 cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc gcccatggct
     5701 tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga gcccatgatg
     5761 aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc acctttataa
     5821 ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg accgaagaaa
     5881 gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt aatcgcgaac
     5941 gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag cactggaata
     6001 ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca gctaaaggca
     6061 aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg ggaacattta
     6121 cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga aaccacaaag
     6181 gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc cagccagaca
     6241 gtaatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac ctggtcgata
     6301 actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct ggcgataaac
     6361 ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca gatgacgaaa
     6421 gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc aaatccgagc
     6481 tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat ccacagcagt
     6541 gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt accaaaatgc
     6601 tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca cagacgcgcc
     6661 agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag aagaatcggg
     6721 atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc gattgccgtg
     6781 ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg ttttgtctgg
     6841 ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa ttaagcgtca
     6901 acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga aataatactg
     6961 acggtttttc tgttttgaat atattggtct aatttaccgc ttaataatgc agcacatgcc
     7021 agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat catactgttt
     7081 ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac gatttcgtaa
     7141 gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt agttcggtgc
     7201 gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc tgactgtacg
     7261 ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc agcaattaaa
     7321 ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc cataatttcc
     7381 agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg gataaaaata
     7441 cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa gttatcacca
     7501 tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt tggcgcacct
     7561 tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga aaccccttgc
     7621 gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc gatcagtaaa
     7681 cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat attttcaaac
     7741 ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg catgcctgtt
     7801 ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat agcaaccggc
     7861 agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg taggtaaccg
     7921 taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa acaaataact
     7981 aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac tctttggcta
     8041 attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat accgcggcat
     8101 attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta gaaccaaaag
     8161 gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca agattataaa
     8221 ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt ctttgtaacg
     8281 tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac tgttcgttct
     8341 tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct accagcacaa
     8401 actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct gcacttaacg
     8461 taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg
     8521 cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg atcatccctg
     8581 acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc accgcctcag
     8641 aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg gattcggaac
     8701 gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc cgcaccacta
     8761 attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc ggttgagtta
     8821 accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc tgaaagacta
     8881 ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt aattacctca
     8941 ttgacggcat gaagtgtatc aaaatgaaat gaacaggata tgtgcgacca ctcacaaatt
     9001 aactttcatt actttccagg gtatcgttat tacaattaaa cacctttaaa tatcaacaag
     9061 ttaaagcata aaaatcagca taaagcctga tttttatcaa aaaaaaatga cggggataac
     9121 catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg ggctgagacc
     9181 atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt taatgagcaa
     9241 gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg cacgtcacat
     9301 tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta aaatttcgaa
     9361 tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt ctgacaggac
     9421 gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg cgacgaatag
     9481 cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa ggtcagacga
     9541 agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga atgttgcttc
     9601 gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt cgtgcgagga
     9661 aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga aaggcttccc
     9721 aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta acaatcacaa
     9781 tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct ccaccaatga
     9841 cgttaataaa atttacagta taagtaatga actgcggaga gtattatctg caattactgc
     9901 attgaatttc tatcaaggcg atgttccttc tgtcatgatc cgaatccaac cggaaaatat
     9961 gagtccattc atcatagata tttctacagg agaacatgat gattatatca tacaaacatt
    10021 agatgtaggc acttttgcac cttttggtga acaatgtact tgctcagccg tcaataaaaa
    10081 agagctggaa tgtattaagg agacgatttc taagtattgc gcaaagttca cccaaaaaga
    10141 agccatctta acacccccta ggcactttaa taaaacctgc attacttcag attgttggca
    10201 aattctcttt tttttcaccc gatcatttta ataatgattt ttattgatta tttataaagg
    10261 ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat ttagtccgtc
    10321 gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca ctagcaccag
    10381 gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac gcgttacaac
    10441 caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac ttacaaaatg
    10501 taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac gcccaaatta
    10561 ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa aaccaggaaa
    10621 tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat gaaatcaaga
    10681 ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc tctctggact
    10741 tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct aatactattc
    10801 taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt caggttaatt
    10861 ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa ataacgcctg
    10921 ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc gaaagttgtt
    10981 taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt gcaattataa
    11041 aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca aaagaaatat
    11101 ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca gagatgaaag
    11161 aattctcatc aaaaaaaata tatgattaca tcgatacaat cattgaagat tatgaaaata
    11221 tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat ttaaacatag
    11281 aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc cggacaatgg
    11341 ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc aatattttac
    11401 cagagaggac tatacgtata attacgacct tttaaatgca atatagactt aataacttct
    11461 tacaaagtct atcattaata ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa
    11521 cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc gctacgctta
    11581 tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg caggataagg
    11641 cgttcatgca gcatccggca tgaataaagc gcactcaaca atctgaaacc cgccggaacg
    11701 gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg gtata
//
LOCUS       DADLSB010000116        11748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.116, whole genome shotgun sequence.
ACCESSION   DADLSB010000116 DADLSB010000000
VERSION     DADLSB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.116"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1362)
                     /gene="cusC"
                     /locus_tag="JN331_003014"
     CDS             complement(<1..1362)
                     /gene="cusC"
                     /locus_tag="JN331_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY7261105.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYLNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEVERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISNASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTAL"
     gene            1519..2202
                     /gene="cusR"
                     /locus_tag="JN331_003015"
     CDS             1519..2202
                     /gene="cusR"
                     /locus_tag="JN331_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY7261106.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2192..3634
                     /gene="cusS"
                     /locus_tag="JN331_003016"
     CDS             2192..3634
                     /gene="cusS"
                     /locus_tag="JN331_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY7261107.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GALDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKCEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            3784..6021
                     /gene="nrfB"
                     /locus_tag="JN331_003017"
                     /pseudo
     CDS             3784..6021
                     /gene="nrfB"
                     /locus_tag="JN331_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            6008..8980
                     /gene="nfrA"
                     /locus_tag="JN331_003018"
                     /pseudo
     CDS             6008..8980
                     /gene="nfrA"
                     /locus_tag="JN331_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            8981..9871
                     /locus_tag="JN331_003019"
     CDS             8981..9871
                     /locus_tag="JN331_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY7261108.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            10054..10815
                     /locus_tag="JN331_003020"
     CDS             10054..10815
                     /locus_tag="JN331_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7261109.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPRLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(10861..10937)
                     /locus_tag="JN331_003021"
     tRNA            complement(10861..10937)
                     /locus_tag="JN331_003021"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10901..10903),aa:Arg,seq:tct)
     gene            11180..>11748
                     /gene="fimZ"
                     /locus_tag="JN331_003022"
     CDS             11180..>11748
                     /gene="fimZ"
                     /locus_tag="JN331_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HAY7261110.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYG"
BASE COUNT         2901 a         3021 c         2999 g         2827 t
ORIGIN      
        1 taaggcggta tacaaataaa tttcgttaac ctgacgggca taattcaggt caagtaaagt
       61 ttgtcgggtt gcaaataaag aacgctcggc atccagcact tccagataac ttaccgcgcc
      121 gtgctgatat aatgcccgcg cccgttgcag ggtaatttgc agcgacgcca gataacgctg
      181 ctgggcgctg atttgatcgt ctaggctttg acgtaatgcc agcgcatctg ccacctcttt
      241 aaaggcgttc tggattttct gttcataatt caccaccgac tgctgctggc gaatttcggc
      301 gatatccaga ttggcctggt tgcgtccggc attaaaaatg gggatctcaa ttttaggaat
      361 aaaattccac atcccgctgc tagcgttaaa taatgatgac agatcgctgc tggcgtttga
      421 tattccgctg gtcaggctga tagacgggaa aaatgccgca cgcgcagcac caatattagc
      481 attagccgcc attaacgcgt gttcagcttc cataatatcc gggcgctgca ataagatttg
      541 cgacgacaag cccgccggta atttaacgct ttgcaggctg ccgctgttta ctgtctgcgc
      601 ttgcggtagc ttgccgtagc ttcccaataa cagttgcaat gcattattcg cctgcgccag
      661 ttccccctga cgtttagcga tgtcactgcg ggtactttct atcaccccgc gagcctgttc
      721 cagcgccaga acattgctgc taccggtcaa cagttgtttt tcgacaaacg catatgactg
      781 ctgataatta cgcagcgttt cttcggctat ttgcaattgc gcatacgcca gctgctgatt
      841 gaaatagctt tgcgcgacat tagaaaccag caaaatatgc accgcgcgct gggcttcctc
      901 agtggctaaa taattttggc gctcgacttc gctcatgttc tttaagcgac cgaaaaaatc
      961 gagatcaaag ctggcgttaa ggccagtcga gaactcccgc gtcgtggctg aatcgccttt
     1021 aagattgccg ctccagctgc cgctgccctc gccattgagc tgtgggtagc ggtcggcatc
     1081 ggtcagacga tattgcgccc gtgcctcctg cactttcagc gtcgccatgc gcaaatcccg
     1141 gttattcacc agcgcctcgc taatcagcgt cttcacctga ttatcaacaa aaaaggtgcg
     1201 ccagcccgcg ttcaggtagt tatccgctgc gttaaccagg ccgttctggc tgagtgagaa
     1261 ctgctgcggt acgggcattg ccggacgctg ataatccggt gccagtgaac aaccagttag
     1321 cgcaagggcc acacaaaatg gcagaagttt acaaggagac ataggctcat aatttctggt
     1381 gattttatgt cgccaacttt actcgccagg ctctgatttt ccggtgacag gaaaatgaca
     1441 aaattgtcat tttgccaata agcgattgcc atctgatccc gctactctag aattgcccgg
     1501 gcaacatgcg gaggaaatat gaaactgttg attgtcgaag atgaaaagaa aaccggagaa
     1561 tacttgacca aagggttaac cgaagccggt tttgtggtcg atttggccga caacgggctg
     1621 aatggctacc atctggcgat gaccggtgat tatgacctga taatcctcga tattatgctg
     1681 ccagacgtga acggctggga tatcgtgcgc atgttgcgct ccgccaataa agggatgccg
     1741 attctgttgc ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct ggagttgggg
     1801 gcagatgact acctggtgaa accattcgct tttgctgaac tgctggcgcg ggtgcgcact
     1861 ctcctgcggc gcggggcggc ggtgattatc gaaagtcagt ttcaggttgc cgatctgatg
     1921 gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc gcatcactct gaccagtaaa
     1981 gaatttactc tgctggagtt ttttctccgc catcagggcg aagtgctgcc ccgctcgctt
     2041 atcgcctcgc aggtatggga catgaatttc gacagcgaca ctaacgccat tgatgtggcg
     2101 gtgaagcggc tacgcggcaa aatcgacaac gactttgagc cgaagctgat tcaaaccgtg
     2161 cgcggcgtgg gttacatgct tgaggtgccg gatggtcagt aagccatttc agcgcccgtt
     2221 ttcgctggca acccgcctga ccttttttat cagcctggcc accatcgcgg cgtttttcgc
     2281 ctttgcatgg atcatgatcc actcagtaaa agtgcatttt gccgagcagg acattaatga
     2341 tttaaaagag attagcgcca cccttgaacg ggtactaaat caccctgacg aaacgcaagc
     2401 ccgacgctta atgacgctgg aagatatcgt cagtggttat tccaacgtgt tgatttccct
     2461 ggcagatagc cacggcaaaa cggtgtatca ctcccccggt gcgcttgata tccgcgagtt
     2521 tgcacgtgat gccatacccg ataaagacgc gcggggtggc gaagtgtatc tcctttccgg
     2581 cccgacgatt atgatgccag gccacggtca cgggcatatg gaacacagca actggcggat
     2641 gattaacttg ccggttggcc cgttggtgga cggcaaaccg atttatacgc tctacatcgc
     2701 gctgtcgatc gattttcatc ttcattacat aaatgatttg atgaataaac ttattatgac
     2761 cgcatcggta atcagcatcc tgatcgtctt tatcgtgctg ttggcggtac ataaaggtca
     2821 cgcgccgatc cgcagcgtca gccgtcaaat ccagaatatc acctcgaaag atctcgacgt
     2881 tcgccttgac ccgcagaccg tgccgattga gctggaacag ctggtactgt cgttcaacca
     2941 tatgatcgag cgtattgagg atgtctttac tcgccagtcc aatttctcag cggatatcgc
     3001 ccacgaaatt cgcacaccaa ttactaatct cattacgcaa acggaaatcg ccctcagcca
     3061 gtcgcgcagc cagaaggagc tggaagatgt gctctactct aatctcgaag agctgacgcg
     3121 aatggcgaaa atggtcagcg atatgctgtt tctcgctcag gccgataaca accagctaat
     3181 ccccgaaaag aaaatgctca acctggcgga tgaagtcggc aaagtgttcg attttttcga
     3241 ggcgctagcg gaagatcgcg gcgtggagtt gcggtttgtt ggcgacaaac gtcaggttgc
     3301 gggcgatccg ttgatgctgc gtcgggcgct aagcaacctg ctttctaatg ccctgcgtta
     3361 tacgccaacc agagagacaa ttgtagtgcg ctgtcagacg gtcgatcacc aggtgcaagt
     3421 ttccgtcgaa aaccccggta cgcccattgc gcccgagcac ttaccgcgat tgtttgaccg
     3481 tttctatcgc gttgacccct cccgccagcg aaaatgtgaa ggtagcggta ttgggctggc
     3541 gatagtgaaa tcgattgttg tcgcgcataa aggcacggtt gcggtaacgt cagatgcgcg
     3601 ggggacaagg tttgttatca cattacccgc ttaattattc agcaaacggc aacttttata
     3661 accagtgtaa aaataacgtg ccgcaatagt catacctatt aatggtaaaa agctgtcaca
     3721 attcataaaa aaccttaata tacgccaccc taaacataac cagcgttaat gtaaggtttt
     3781 tgtgtggact ggcttcttga tgtttttgct acctggctct acggcttaaa agtaatcgcg
     3841 ataacgttag cggtcatcat gttcatcagc gggctggacg atttttttat tgatgtcgtc
     3901 tactgggtac gccgcattaa acgcaagttg agtgtttatc gccgctaccc gcgaatgagt
     3961 taccgcgaac tgtataaacc agatgaaaaa ccgttagcga ttatggttcc ggcgtggaat
     4021 gaaacgggcg tcatcggcaa tatggccgag ctggcggcga ccacgctcga ctacgaaaac
     4081 tatcatatct ttgttggcac ctaccccaac gaccccgata ctcaacgtga tgttgacgaa
     4141 gtgtgcgctc gctttccgaa cgtgcataag gtagtctgcg cgcgtcctgg tcccaccagc
     4201 aaagccgact gtctgaacaa cgtgctggac gccatcaccc agtttgaacg tagcgccaat
     4261 ttcgcttttg ctggttttat tctgcatgac gccgaagatg tgatttcacc gatggaattg
     4321 cgtctgttca actatctggt cgagcgtaaa gatctgattc agatcccagt gtatccgttt
     4381 gaacgcgaat ggacgcactt caccagcatg acttacattg atgagttttc agaactgcat
     4441 ggcaaagatg ttccggtgcg tgaagccctc gccggacaag tgcccagcgc aggcgtcggc
     4501 acctgtttca gccgccgcgc cgtgaccgca ctgttagctg acggtaacgg tattgctttc
     4561 gacgtgcaga gtcttactga agattacgac attggcttcc gcctgaaaga aaaaggtatg
     4621 acggaaattt ttgtccgttt tccggtggtg gacgaagcca aagaacgcga gcagcgtaaa
     4681 tttttacagc acgcacggac gtcaaacatg atctgcgtgc gcgaatattt ccccgatacc
     4741 ttttcgactg cggttcgaca aaaatctcgc tggatcatcg gcattgtttt ccaaggcttt
     4801 aaaacccata aatggacctc cagcctgacg ctgaactact ttctctggcg cgaccgcaaa
     4861 ggggcaatca gtaactttgt cagcttcctc gcgatgctgg tgatgatcca gcttttgctg
     4921 ttgctggcgt atgaaagttt gtggcccgat gcctggcatt tcctttctat ttttagcggc
     4981 agcgcatggt taatgaccct gctgtggcta aactttggct tgatggttaa ccgcatcgtg
     5041 cagcgagtga ttttcgtcac tggctactac ggcctcacgc agggcctgct ttccgtcctg
     5101 cgtcttttct ggggcaacct gattaacttt atggctaact ggcgcgtgct gaaacaggtt
     5161 cttcagcacg gcgatccgcg tcgcgtggcg tgggataaaa caacgcatga cttccccagc
     5221 gtcactggcg atacccgctc gttgcgcccg ttaggtcaaa ttctgctgga aaatcaggtc
     5281 atcactgaag aacaactcga tacagcactg cgtaatcgcg tcgaaggtct acgcctgggc
     5341 ggttcaatgc tgatgcaggg gctgattagc gccgagcagc tggcacaggc gctggcagag
     5401 caaaacggcg tggcgtgaga atccatcgat gcctggcaga tcccgtcctc gctgattgcc
     5461 gaaatgccag cctccgtagc gctgcattat gcagtactgc cgctgcgtct ggaaaatgat
     5521 gagttaattg tcggcagtga agatggtatt gacccggttt cgctggcggc cctgacgcgt
     5581 aaagtcggac gcaaagtgcg ttacgtcatt gttctgcggg gacaaattgt cacagggtta
     5641 cgccactggt atgcacgccg acgcggtcac gatccgcggg caatgttgta caatgcggtt
     5701 cagcatcagt ggctcacgga acagcagacc ggtgaaatct ggcggcaata tgtgccgcat
     5761 cagttcctgt tcgccgaaat actgaccacg ctcggtcata ttaatcgttc agcaattaac
     5821 gtgttgttat tgcgccatga acgcagttct ctgccgctcg gcaagttttt ggtcaccgaa
     5881 ggcgttatca gccaggaaac gttggatcgc gtcctgacaa ttcaacgcga attacaagtt
     5941 tcgatgcaat cactattact caaagcaggt ttaaacacag aacaggttgc gcaactggag
     6001 tccgaaaatg aaggagaata accttaatcg cgtcatcgga tggtctggtt tactgctgac
     6061 gtctttattg agtaccagcg cactcgcaga caatatcggc accagcgcag aagagctggg
     6121 gctgagcgat tatcgccatt ttgttattta tccccgtctc gataaggcgc tgaaggcaca
     6181 gaaaaataac gacgaagcaa ccgccatccg cgaatttgaa tatatacacc agcaggtgcc
     6241 ggataatatt ccgctgactt tataccttgc ggaagcctat cgtcattttg gtcatgatga
     6301 ccgggcgcgg ctgttgcttg aggatcaact gaaacgtcac ccaggagatg cccgacttga
     6361 gcgcagtctg gcggctattc cagttgaagt gaaaagcgtt acgactgttg aagaactgct
     6421 tgcccagcaa aaagcgggcg acgctgcgcc gaccctgcgt tgtcgcagtg aagtcgggca
     6481 gaatgccctg cggctggcac aattacctgt cgccagagcg caactgaacg atgcgacgtt
     6541 tgctgcatcg ccggaaggaa aaacgctgcg aaccgatctg ctgcaacggg caatctacct
     6601 gaaacaatgg tcccaggcag atacgctata caatgaagca cgccagcaga acacattaag
     6661 cgcggcagaa cgccgtcagt ggtttgacgt gcttcttgcc gggcagctgg acgatcggat
     6721 cctggcactg caatcacagg ggatcttcac cgatcctcag tcatatatta cttacgcgac
     6781 cgcgctggct tatcgtggcg aaaaagcacg cctccagcat tatctcattg aaaataagcc
     6841 gctgtttacc acgggcgcac aagagaaaag ttggctctat ctgttatcta aatacagcgc
     6901 caaccccgtt caggcattgg cgaattatac ggtgcagttt gccgataacc gccagtatgt
     6961 tgttggcgcg acgctaccgg tgctgttaaa agaaggtcag tacgacgcag cgcaaaaact
     7021 gctcgccacc ctccccgcca atgaaatgct tgaggagcgt tatgctgtca gcgtggcgac
     7081 ccgtaacaag gctgaatctc tgcgtctggc acgattgctg tatcagcaag aaccgacaaa
     7141 tcttccccgc ctggatcaac tgacctggca actgatgcag aacgagcagt cacgcgaagc
     7201 tgccgattta ttgctgcaac gctatccttt ccagggcgat gcgcgtgtca gccagacttt
     7261 aatggcgcga ctggcgtctc tgctggaaag tcatccttac ctagcaacgc cggcgaaggt
     7321 ggcgatttta tcgaaaccct taccgctggc ggagcaacgt cagtggcaaa gtcagttgcc
     7381 gggtattgca gataattgcc cggcaatagt tcgcttgctg ggcgatatgt cgccttccta
     7441 cgatgctgcc gcctggaacc gtctggcaaa gtgttatcgg gacacgctac ccggtgtggc
     7501 gttgtatgca tggcttcagg ccgaacaacg acaaccgagc gcctggcaac atcgtgcggt
     7561 agcctatcag gcgtatcagg ttgaggacta cgccaccgca ctggcggcct ggcagaaaat
     7621 cagtcttcac gacatgagca atgaggatct gctcgccgcc gccaacaccg ctcaggcagc
     7681 gggaaatggc gcggctcgcg atcgctggct tcaacaggca gaacaacgtg ggctgggaaa
     7741 caatgctcaa tactggtggc tgcatgcgca acgttatatc ccagctcagc cagaactcgc
     7801 actgaacgat ctcacgcgct caatcaatat tgccccttct gccaacgctt acgttgcgcg
     7861 ggcgacaatt tatcgccaac gtcataatgt tccggccgcg gtgagtgatt tgcgcgccgc
     7921 gctggaactg gaaccgaata atagcaacac ccaggcagcg cttggttacg ccttgtggga
     7981 tagcggtgat atcgcacagt cgcgggaaat gctcgaacag gcgcataaag ggcttccgga
     8041 cgatccggca ctgatccgac aactggccta cgtgaaccag cgtctggatg acatgcctgc
     8101 gacgcagcac tacgcccggc tggtgattga tgacattgat aatcaggcgc tgataacccc
     8161 actgacccca gagcaaaatc agcaacgctt caatttccgc tgtttgcatg aggaggtcgg
     8221 tcgccgctgg acgttcagtt tcgattcttc catcggcttg cgttccggcg caatgagtac
     8281 cgctaacaat aatgtcggcg gtgcagcgcc tgggaaaagc tatcgtagct acggacaact
     8341 ggaagccgag taccgcatcg gacgcaatat gctgctggaa ggcgacctgc tctcagttta
     8401 tagccgcgtc tttgccgata ccggagaaaa cggggtgatg atgccggtga aaaatccgat
     8461 gtccggcacc ggtctgcgct ggaagccgct gcgcgatcag atctttttcc tcgccgtcga
     8521 acagcagttg ccgctgaacg ggcaaaatgg cgcatccgat accatgctgc gcgccagcgc
     8581 ctcattcttt aatggcggca aatacagcga cgaatggcac ccgaacggtt caggctggtt
     8641 tgcccaaaac ctgtacctcg atgcggcgca atatatccgc caggatattc aggcgtggac
     8701 ggcagattat cgcgtcagct ggcatcagaa ggtagctaac ggacagacta ttgagcctta
     8761 cgctcacgtt caggacaacg gctatcgtga taaaggcact cagggcgcgc agcttggcgg
     8821 tgtcggggtc cgctggaata tttggaccgg cgagacgcac tacgacgcct agccgcacaa
     8881 agtcagtctc ggcgtcgagt atcaacatac ctttaaggcg attaatcaac gtaacggaga
     8941 gcgcaacaac gcgtttctca ccattggagt acactggtaa atgcgtaagt tcattttcgt
     9001 attgctgaca ctgcttttgg tcagcccttt ttcctttgcg atgaaaggta ttatctggca
     9061 accacaaaac cgagatagtc aggttaccga tacccagtgg caggggctga tgagtcagtt
     9121 acgttttcaa ggcttcgata ccctggtttt gcaatggacc cgttacggcg atgcatttac
     9181 ccagccagaa cagcgcacgt tattgtttaa gcgtgccgca gctgcgcaac agactggcct
     9241 gaagctgatt gtcgggctga acgccgatcc ggaatttttt atgcaccaga aacagtcgtc
     9301 tgcagcgctg gaaagctatc ttaatcgcct gctggctgcc gatctccagc aagccagatt
     9361 atggagcgcc gcgcctggca taacgccgga tggctggtac atcagcgcgg aaattgacga
     9421 cctgaactgg cgcagcgaag ccgcccgtca acctttgcta acatggttaa acaacgcgca
     9481 gcggctgatt agcgatgttt cagcaaaacc ggtttatatc agtagttttt tcgccggaaa
     9541 catgtcgccc gatggctatc gccaactgct ggaacaagtt aaagcaaccg gagttaatgt
     9601 ctgggtacag gatggcagcg gcgtggataa acttaccgct gaacagcgtg aacgttatct
     9661 acaggccagc gccgattgcc aaagctccgc cccagcaagc ggcattgttt atgaactgtt
     9721 tgtcgccggc aaaggcaaaa cctttacagc gaaaccgaaa ccggacgcag aaattgcctc
     9781 gctgttagcg aaacgttcct cttgcggtaa agacatgctc tatttctctc tgcgctattt
     9841 gcccgtcgcg cacggcattc tcgagtatta aatcccctcc aggtaagtcg ggtacgacct
     9901 ggcttacctc tttcgctctc gtcagaattc catcaagatt aattgctaaa aagcatgcta
     9961 aagaaagaag atcgttatcg gcgaacgggc cgatcactca tgcaggaagc atagatcctt
    10021 tgagcggagt ttctgatatc tgaggagtgc gaaatgcaat tgagcagcag tgaaccttgc
    10081 gtggtgatcc tgaccgaaaa agaggtagag gtaagcgtca ataaccatgc tacgtttacc
    10141 cttccgaaaa actacctggc cgccttcgcg tgcaacaata acgtcattga actctcaacg
    10201 ttaaatcacg tattaatcac ccacatcaac cgtaacatca tcaacgatta tctgttgttt
    10261 ttaaataaga aattaacctg tgtaaagccc tggtcgcggc tggcaacccc ggttatcgct
    10321 tgtcatagcc gtacaccgga agtgttccgg ctagccgcca accacagcaa gcagcaaccc
    10381 agcaaaccct gcgaagcgga gttgacgcgc gcattacttt ttaccgtatt gtctaacttt
    10441 cttgagcaat cgcggtttat tgccctactg atgtatatct tacgcagcag cgtccgcgac
    10501 agcgtctgcc gcattattca aagcgatatt cagcattact ggaatctgcg aattgtcgcc
    10561 agttcgctat gtttaagccc caggctgctc aaaaagaaat taaaaaacga aaataccagc
    10621 tatagccaga ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt attgatggat
    10681 aacaaaaata tcactcaggt ggcgcaatta tgtggctata gcagcacgtc gtactttatc
    10741 tctgttttta aggcgtttta cagcctgaca ccgttgaatt atctcgccaa acagcgacaa
    10801 aaagtgatgt ggtgaagggc aaagcggaaa cggataagac gggcataaat gaagaagaaa
    10861 tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt tgctctatcc
    10921 aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat tatacggtca
    10981 acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga ccgcgtctga
    11041 ttttctgatt tatttcgcta tagcggcaaa caaacgcaca ccgctgcgcg tctgaatcaa
    11101 gaaaacccgt attttcatgt atcaaagtac aatttaccgc cctaacggaa aattgtccgc
    11161 tcctatgaga ctggtaacta tgaaaccaac gtcggtgatc attatggata ctcatcctat
    11221 catcagaatg tctattgaag ttctgttgca aaaaaacagt gaattgcaga ttgtcctgaa
    11281 aacggatgat taccgcataa ccatcgatta tctccgaacc cgtcctgttg atttaatcat
    11341 tatggatata gacttgcccg gaacagacgg ttttaccttc ctgaaaagga tcaaacaaat
    11401 ccagagcaca gtgaaagtgt tatttttatc atcgaaatca gaatgctttt atgctggcag
    11461 agcgatacaa gctggtgcta acggttttgt cagtaaatgc aatgatcaga atgatatttt
    11521 tcatgccgtt cagatgatcc tctccggata cacgtttttt cccagcgaaa cgcttaacta
    11581 tataaaaagc aataaatgta gtacgaatag ttcaacggtc actgtgctat ctaatcgtga
    11641 agtgaccata ttacgttatc tggttagcgg attatctaat aaagaaattg ccgataagtt
    11701 attgcttagc aataaaacag ttagtgcgca taaatctaat atttatgg
//
LOCUS       DADLSB010000117        11726 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.117, whole genome shotgun sequence.
ACCESSION   DADLSB010000117 DADLSB010000000
VERSION     DADLSB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.117"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..2266
                     /locus_tag="JN331_003023"
                     /pseudo
     CDS             1..2266
                     /locus_tag="JN331_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2267..3916
                     /locus_tag="JN331_003024"
                     /pseudo
     CDS             2267..3916
                     /locus_tag="JN331_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
     gene            3921..4697
                     /locus_tag="JN331_003025"
     CDS             3921..4697
                     /locus_tag="JN331_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY7261111.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4972..7821
                     /gene="rcsC"
                     /locus_tag="JN331_003026"
     CDS             4972..7821
                     /gene="rcsC"
                     /locus_tag="JN331_003026"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY7261112.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEVQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8021..8671)
                     /gene="rcsB"
                     /locus_tag="JN331_003027"
     CDS             complement(8021..8671)
                     /gene="rcsB"
                     /locus_tag="JN331_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY7261113.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8688..11360)
                     /gene="rcsD"
                     /locus_tag="JN331_003028"
     CDS             complement(8688..11360)
                     /gene="rcsD"
                     /locus_tag="JN331_003028"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY7261114.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTLRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESDVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2844 a         2839 c         3156 g         2887 t
ORIGIN      
        1 aattaacctc gtcgctgaaa ggtaaacctg tttctgcgca gctaacggta ggcgtggtcg
       61 atgaaatgat ctacgcgctg caaccagaaa tcgcgccgaa tatcggcaaa tttttctatc
      121 cgctggggcg taacaatgtg cgtaccagct ccagtctgtc gtttatcagc tacgaccagg
      181 cgctctccag cgagccggtt gcgcctggcg cgactaaccg cagcgagcgg cgagtaaaaa
      241 tgcttgaacg tccacggcgt gaagaggtgg ataccgcggc atggatgccg tcactcacaa
      301 ccgataaaca aggcaaagcg tatttcacgt tcctgatgcc tgattcgtta acccgctggc
      361 gtatcaccgc gcgtgggatg aacggcgacg ggctggtcgg gcaggggcgt gcttatctgc
      421 gttcggaaaa aaatctctac atgaagtgga gtatgccaac ggtgtatcgc gtgggcgata
      481 aaccggcggc aggactgttt atcttcagtc agcaggataa cgaaccggtg gcgctggtga
      541 ctaaatttgc aggcgctgag atgcgccaga cgttgacgct gcacaaaggg gcgaattata
      601 tttcgctgac gcagaatatt cagcaatctg gcttgttaag tgcagaactg caacaaaatg
      661 ggcaagtgca ggacagcatt agcacaaaac tgtcttttgt ggacaacagc tggcccgttg
      721 aacagcagaa aaatgtcatg ctcggcggtg gcgagaacgc gctgatgttg cccgagcagg
      781 cgagtaatat ccggctacaa agtagtgaaa cgccgcagga gattttccgc aacaatcttg
      841 atgcgttagt cgatgaaccg tggggtgggg tgatcaacac cggtagccgt ctgatcccgc
      901 tcagtctcgc ctggcgttcg cttgccgatc atcaaagtgc cgccgctaac gacattcgtc
      961 agatgattca ggataaccgt ctgcggctga tgcaactggc ggggcccgga gcgcgcttta
     1021 cctggtgggg tgaagatggc aatggtgacg ccttccttac ggcatgggca tggtacgccg
     1081 actggcaggc cagccaggcg ctcggcgtaa cgcaacaacc ggaatactgg cagcatatgc
     1141 tcgacagtta tgccgagcag gcgggtaaca tgccgttatt gcatcgggcg ctggtgctgg
     1201 cgtgggcaca ggagatgaat ctgccgtgca aaacgttgtt gaaagggttg gatgaagcta
     1261 tcgcccggcg cggaactaaa gatgaagatt tctctgagga agacatccgc gatatcaatg
     1321 acagcctgat cctcgataca ccggaatctc cactggcaga tgcggtggca aacgtcttaa
     1381 ccatgacgtt gctgaaaaaa gcgcagttga agtccacggt gatgccacag gttcagcaat
     1441 atgcgtggga taaagcggca aacagcaatc agccgctggc gcacacggtt gtgctgctca
     1501 atagcggtgg tgacgctacc cagacggccg ctattttaat ggtttgaccg ctgagcaatc
     1561 cactattgag cgcgcgctgg ccatgaactg gctggcgaaa tatatggcga caatgcctcc
     1621 agttgttttg cctgcgcctg cgggcgcatg ggctaaacat aagttaactg gagggggcga
     1681 agactggcgt tgggttggtc agggtgtgcc ggacattctc tcttttggtg acgaattatc
     1741 gccgcaaaat gtgcaggtcc gctggcgtga accggcaaaa acggctcaac aaagtaacat
     1801 tccggtgacc gttgaacgcc agttgtatcg gcttatcccc ggtgaagaag agatgagctt
     1861 tactctgcag ccggtgacca gcaatgagat tgacagcgat gcgctgtatc tcgatgaaat
     1921 tacgcttacc agcgagcagg atgcagttct gcgctatggt caggtggaag taccgctccc
     1981 gccgggagcc gacgttgagc gcacaacatg gggcatttca gtcaataaac ccaacgccgc
     2041 gaaacagcag gggcaattgc tggaaaaagc gcgtaatgaa atgggcgaac tggcctatat
     2101 ggtgccggtg aaagaactga cgggaacggt cactttccgc catttgctgc gcttctcgca
     2161 aaaagggcaa ttcgttctgc ctcctgctcg ttatgtgcgt tcctatgcac ctgcgcagca
     2221 aagtgtcgcg gcagggagtg aatggaccgg gatgcaggtg aaataagtga actggcgcag
     2281 aatcgtctgg ctattggcgt tggttactct gccaacgctt gcggaggaga cgccgttaca
     2341 actggtgctg cgcggtgcgc agcacgatca actgtatcag ctgtcgtcat caggcgtgac
     2401 caaagtctca gcattacctg actcgctcac cacgccgtta gggagtttgt ggaaactgta
     2461 tgtttacgcc tggctggagg atacccatca accggagcag ccctaccagt gtcgcggaaa
     2521 ctccccggaa gaggtttatt gttgtcaggc tggggagagc ataacgcgcg ataccgcgct
     2581 ggtacgttcc tgtgggctct attttgcgcc gcagcggttg catattggcg cagatgtgtg
     2641 ggggcaatac tggcaacagc gtcaggcacc tgcgtggtta gcgtctctta caacgctgaa
     2701 gccagaaacc tcggtaacgg ttaaatcgct gcttgattca ttagcgacgt tacctgcgca
     2761 aaacaaagct caggaagtct tactggatgt ggtgctggac gaggcgaaaa tcggtgtcgc
     2821 atccatgctc ggtagtcggg tacgggtgaa aacctggagc tggtttgccg atgataaaca
     2881 ggaaatccgt cagggtggtt ttgctggctg gttaaccgat ggtacaccgc tgtgggttac
     2941 tggtagtggc accagcaaaa cggtactaac ccgttatgcg acggtgctta atcgggtttt
     3001 gccagtgccg acgcaggttg ccagtgggca atgtgtagag gtggagctgt ttgcccgcta
     3061 tccgctgaag aaaatcacag cggaaaagag tacgacggca gtaaaaccca gcgtacttaa
     3121 tgggcgctat cgtgtgacgt ttactaatgg taaccacatt acgtttgtca gccacggtga
     3181 gacgacctta ctgagcgaaa agggcaaact gaaattgcag tcgcatctcg atcgtgaaga
     3241 gtacgttgcc agagttctgg atcgcgaggc gaaaagtacg ccacctgagg cagcgaaagc
     3301 catgaccgtg gcgatacgta cttttttaca gcaaaacgcc aatcgggaag gtgattgcct
     3361 gaccatcccc gacagcagcg ccacgcagcg tgtttctgct tcgccagcga ccacgggggc
     3421 gcgaacgatg accgcctgga cgcaggagct catctatgca ggcgatccgg ttcattatca
     3481 tggcagtcgc gctaccgaag ggacactttc ctagcggcaa gccacggccc aggcggggca
     3541 gggggagcgt tacgaccaga tactcgcgtt tgcttatccc gacaacagcc ttagccgctg
     3601 gggagctcca cgctcaacct gccagttatt acccaaagcg aaagcctggc tggcgaaaaa
     3661 aatgccgcag tggcggcgta tattacaagg cgagacgggg tacaacgaac cagacgtgtt
     3721 tgcggtctgt cgtttagtct caggtttccc ctataccgat cggcagcaga aacggctgtt
     3781 tattcgtaac ttctttacgc ttcaggatcg gctggattta acccatgagt atctgcacct
     3841 ggcctttgat ggttatccca ccgggcttga tgagaactat atcgaaacgc tgacccgaca
     3901 attattaatg gactaatgct atgcgaaaga tttttcttcc gttgttactg gtggcacttt
     3961 cgcctgtcgc tcacagtgaa ggtgtgcagg aagtcgagat tgacgcaccg ctctctggct
     4021 ggcatccggc agagggcgaa gatgccagtt tttcacaatc gatcaattac ccggcgtcgt
     4081 ccgtcaacat ggcagatgat caaaatatct cagcgcagat ccgcggcaaa ataaagaatt
     4141 atgctgcggc gggtaaagtt cagcagggcc ggctggtggt caacggtgcc agtatgccgc
     4201 agagaattga atccgatggt tcatttgcac gcccttatat tttcactgaa ggcagcaaca
     4261 gcgtgcaggt catcagcccg gatgggcaaa gccggcaaaa aatgcagttt tactcaacgc
     4321 cgggtacagg aactattcgt gcacgtttac ggctggttct ctcgtgggat acggacaata
     4381 ccgatctcga tcttcatgtt gtcacgcccg atggcgaaca cgcctggtac ggtaacaccg
     4441 tgctgaaaaa cagtggtgca ctggatatgg atgtcacgac ggggtacgga cccgagattt
     4501 tcgccatgcc agcaccagtt cacgggcgct atcaggtgta tatcaactac tatggtgggc
     4561 gcagcgaaac ggaattgacg accgcccaac tgacgctgat caccgatgaa ggatcggtca
     4621 atgagaaaca ggaaacattt attgtaccga tgcgtaatgc tggcgagctg acgctggtga
     4681 aaagttttga ctggtgatcc atcccttcac acaacctggc tctctctttt ttcagataag
     4741 actgctgggc gatctgctga cgccaggata taaccattaa caagcgtcgg cagattggtc
     4801 atttcttatt tttcgtactg agaactgtcc atctactccg gctttcctgg ttaaggcaat
     4861 ggtttctaag cgatacgcat tattgcaatg gtgacagaac attgcggtaa cacgctttta
     4921 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
     4981 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
     5041 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
     5101 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
     5161 caacgcacct ctgatgtgat gaaagagctt aagtacatcg ccgaaaatcg cttatcggca
     5221 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
     5281 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
     5341 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctcaac
     5401 cgggtatttt taatcggcag tgataacctc tgcatggcca atttcggtct gcgtgatatg
     5461 ccagtggaac gcgataccgc gttgaaagcg ttgcatgaac gcatcaataa atatcgaaat
     5521 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
     5581 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
     5641 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
     5701 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
     5761 attaagggcg atcctcgctg gatgcaggaa cgctcttggt ttggctatac ggaagggttc
     5821 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
     5881 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
     5941 gtgcttgccg gagctgcatt gtttactctc gcgcggatgt acgagcgacg tattttcatt
     6001 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
     6061 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
     6121 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
     6181 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
     6241 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
     6301 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
     6361 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
     6421 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
     6481 gtcgatcgtc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
     6541 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
     6601 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
     6661 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
     6721 atgcgtttac agcaggtcat ctccaacctg ttgagtaatg ccataaaatt caccgatacc
     6781 ggctgtatag ttttgcatgt tcgcgctgat ggcgattatc tctctatccg tgttcgcgat
     6841 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
     6901 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
     6961 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
     7021 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
     7081 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
     7141 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagtgcagga accgactccc
     7201 gaagatgtgt taatcactga cgaggtagtg agtaaaaaat ggcagggcag agcggtagtg
     7261 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
     7321 agtgtggctg ctccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
     7381 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataacgac
     7441 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
     7501 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gcgtcgatgc gcttaatgta
     7561 cttagtaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
     7621 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
     7681 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctc
     7741 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
     7801 aggaaatcgc gggattcgta ggacggataa ggcgtttacg ccgcatccgg cattcagtgc
     7861 aaatgccaga taagacactg acgcgtctta tctggcctac tttaatgtaa tctgttgaaa
     7921 taatgggaat cgtaggccgg ataaggcgtt tacgccgcat ccggcaatcg gtgcaaatgc
     7981 cagataagac actaacgcgt cttatctggc ctacagatga ttagtcttta tctgccggac
     8041 ttaaggtcac agaagagaga taattcagca gggcgatatc gttctcgacg cccagcttca
     8101 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
     8161 tctcggtcac taggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
     8221 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
     8281 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
     8341 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
     8401 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
     8461 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
     8521 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
     8581 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
     8641 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
     8701 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
     8761 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
     8821 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
     8881 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
     8941 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
     9001 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
     9061 acgcacaatt gaccaggccc aatttcccgt acgtcagact catcatcgct taaaagcaag
     9121 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
     9181 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaacaggcg agtgacaata
     9241 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
     9301 tcttcctctt caacttccgg gtcagcggcg agcattttga tatgcacaga gtagcgtgta
     9361 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
     9421 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
     9481 aacgggaagt gcaaattatc catttcatga atacttacgc cttctcccgt gtccagaatg
     9541 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
     9601 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
     9661 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
     9721 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
     9781 aacagcaccg tctcactttt ccagctatca tccgcaagca tgttcgctaa ctgtatttct
     9841 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
     9901 agtttagccg cactctccgc cagggactgt gcgggttctt tcagcgcatc gccaatgttt
     9961 ttcataaagg tcatccgccc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    10021 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    10081 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    10141 atcccctgat gctgttccgc catggtggtg atgttttgca gattcagatg cggcagcaaa
    10201 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    10261 ccgagcggca acagtgagac tatctcttca ttgattgccc gtaaaatgcg caattcgtta
    10321 ttgaccgccg tgctggggac gctttctgta ctgcggctgg agaaatggcg gaatgtggta
    10381 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    10441 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    10501 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    10561 gtcccttctt tctcattatc attgtttccc gttgccgtcg cgtctggctc aaggcggaaa
    10621 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    10681 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    10741 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    10801 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    10861 gtgagatctt taaggggtag ggttgagatc agcaccagac tgttatcctg accattcagg
    10921 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    10981 cgcagagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    11041 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    11101 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    11161 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    11221 ttcagccagg cgttaacggc actttgtacc attaccccca tcgtcaccag taacacaatg
    11281 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    11341 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    11401 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    11461 cccgtgatat ttatcatcct gtatgggggg aaatagatgt acaagcgcca ttttgctgta
    11521 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacaaagat tgctggaaat
    11581 tatgctgatg ttaattattt gtgaaatagt taacaagcgt tatagttttt ctgtggtagc
    11641 acagaataat gaaaagtgtg taaagaaggg taaaaaaaaa cgaatgcgag gcatccggtt
    11701 gaaatagggg taaacagaca ttcaga
//
LOCUS       DADLSB010000118        11666 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.118, whole genome shotgun sequence.
ACCESSION   DADLSB010000118 DADLSB010000000
VERSION     DADLSB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.118"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1616
                     /gene="ftsK"
                     /locus_tag="JN331_003029"
     CDS             <1..1616
                     /gene="ftsK"
                     /locus_tag="JN331_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7261115.1"
                     /translation="QPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKP
                     TTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFE
                     LNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVL
                     DNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLY
                     KAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALLWCVNEMERRYKLMS
                     ALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADL
                     MMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKI
                     DSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQY
                     VDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAAR
                     IIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            1771..2382
                     /gene="lolA"
                     /locus_tag="JN331_003030"
     CDS             1771..2382
                     /gene="lolA"
                     /locus_tag="JN331_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY7261116.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            2393..3736
                     /locus_tag="JN331_003031"
     CDS             2393..3736
                     /locus_tag="JN331_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY7261117.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            3827..5119
                     /gene="serS"
                     /locus_tag="JN331_003032"
     CDS             3827..5119
                     /gene="serS"
                     /locus_tag="JN331_003032"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY7261118.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            5359..7803
                     /gene="dmsA"
                     /locus_tag="JN331_003033"
     CDS             5359..7803
                     /gene="dmsA"
                     /locus_tag="JN331_003033"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HAY7261119.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNHTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            7814..8431
                     /gene="dmsB"
                     /locus_tag="JN331_003034"
     CDS             7814..8431
                     /gene="dmsB"
                     /locus_tag="JN331_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY7261120.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            8433..9296
                     /locus_tag="JN331_003035"
     CDS             8433..9296
                     /locus_tag="JN331_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY7261121.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(9332..9958)
                     /locus_tag="JN331_003036"
     CDS             complement(9332..9958)
                     /locus_tag="JN331_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7261122.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            10273..11421
                     /locus_tag="JN331_003037"
     CDS             10273..11421
                     /locus_tag="JN331_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7261123.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNSSIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         2774 a         2822 c         3158 g         2912 t
ORIGIN      
        1 aacagccgca acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg
       61 cgccacagcc gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc
      121 cgttgcacaa accgacgacg ccgctgcctt ctttggattt gcttacaccg ccgccgagcg
      181 aagtggagcc ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc
      241 tggctgattt ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc
      301 gctttgaact taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg
      361 accttgcccg ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac
      421 cctatgtagg tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt
      481 tggataacgc caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata
      541 tcgccggtga gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga
      601 ctaccggttc cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag
      661 cacagccaga agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt
      721 atgaaggcat tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg
      781 cgctgctctg gtgtgttaac gagatggagc ggcggtataa actgatgtct gcgctgggtg
      841 tgcgtaatct ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga
      901 ttccagaccc gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa
      961 aagaaccata cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta
     1021 aaaaagtgga agagctgata gcacgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc
     1081 tcgtactggc aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata
     1141 ttccgacccg tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc
     1201 aggctggcgc ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca
     1261 cgttgccggt acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc
     1321 aggactggaa agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa
     1381 gcgaaggtgg tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg
     1441 cggtgcagtt tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc
     1501 gcattggtta taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca
     1561 gcgaacaggg gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa
     1621 tgcattgccg gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag
     1681 tattttcttc tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg
     1741 atcgggagtg acgtaatttg aggaataatg atgaaaaaaa ttgccatcac ctgtgcatta
     1801 ctctcaagct tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc
     1861 ctggataaag tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc
     1921 gcggcggtgc aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg
     1981 catatgacac aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat
     2041 aacccgttcg ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg
     2101 tttatgctga ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat
     2161 ggcgatgact ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt
     2221 aacgtgggac gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc
     2281 agcagttatc agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc
     2341 accccgccgc aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa
     2401 tctgtcgctc gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga
     2461 aaatttagca cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg
     2521 cgctatcgaa gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa
     2581 aacaaccctc gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc
     2641 cgtcacctct ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa
     2701 tgcaggtcgc cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca
     2761 ggatgcattt ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga
     2821 aaacccgtcg tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa
     2881 atccctgagt acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg
     2941 tggctatggt ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact
     3001 ggtgaatggc gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga
     3061 agtctatgat agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga
     3121 acgtagcgcc cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca
     3181 taagtcggta cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac
     3241 cgctggtggc gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt
     3301 cggtaatgcc gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg
     3361 cgttggcccg gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc
     3421 gccaaaaagc aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg
     3481 cccggattat gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat
     3541 gggctacggg caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga
     3601 ggtttacttc ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct
     3661 tgaaggcaag attggcgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat
     3721 aaaacgctac cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca
     3781 ggctgtggcc acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa
     3841 tctgctgcgt aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct
     3901 ggatgtagat aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga
     3961 aaacctgcaa gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg
     4021 ggaagatatc gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc
     4081 aaaagccgag ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa
     4141 cctgcctgca gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg
     4201 ctggggtacc ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat
     4261 gcactctggc ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat
     4321 gaaagggcag attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac
     4381 cgaacagcat ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta
     4441 cggtacgggt cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga
     4501 agaagcagac accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct
     4561 ggtgcgcggt gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc
     4621 atgcttccgt tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca
     4681 ccagttcgac aaagttgaaa tggtgcagat tgtgcgccca gaagactcaa tggcggcgct
     4741 ggaagagatg actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa
     4801 aatcatcctt tgcactggcg acatgggctt tggcgcttgc aaaacttacg acttggaagt
     4861 atggatcccg gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt
     4921 ccaggcacgt cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt
     4981 tcataccctg aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa
     5041 ctatcagcag gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg
     5101 actggaatat attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct
     5161 ttttttgtct ccctttgata ccgaacaata attactcctc acttacacgt aatactactt
     5221 tcgagtgaaa atctacctat ctctttgatt ttcaaatcat tcgatgtata caagcctata
     5281 tagcgagctg ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc
     5341 aagcttgagt gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggtg
     5401 agtcgccgtg gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca
     5461 ttaacattac cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca
     5521 gacgaaaagg ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt
     5581 atgcacgtcg tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat
     5641 tacgacggcc tgcaccaggt tcgcgcctgc ctgcgtgggc gttccatgcg tcgccgtgtc
     5701 tacaatccgg accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa
     5761 ttcgagcgca ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg
     5821 atcaaagagt acggcaacga gtctatctat ctgaactatg gcaccggtac gctgggcggc
     5881 accatgaccc gctcctggcc gccgggaaat accctggtcg cgcgcctgat gaactgctgc
     5941 ggcggttatc ttaaccatta cggtgactac tcttccgcgc aaattgcgga aggtttgaac
     6001 catacctacg gcggctgggc agatggtaac agcccgtcgg atatcgaaaa cagtaaattg
     6061 gtagtgctgt ttggtaataa cccaggcgaa acgcgaatga gtggcggtgg ggtgacttac
     6121 tatcttgaac aggcacgcca gaaatctaat gctcgcatga tcatcatcga tccgcgctat
     6181 accgacaccg gtgccgggcg cgaagatgag tggatcccta ttcgtccagg aacagatgcc
     6241 gcactggtta acggtctggc gtacgtcatg atcactgaaa acctggtgga tcaggcattc
     6301 ctcgataaat attgcgttgg ctatgacgag aaaaccctgc ccgccagtgc gccgaaaaat
     6361 ggtcattata aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa
     6421 tgggcctcgc aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc
     6481 ggcagtacca aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt
     6541 gaaatcgcaa cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaat
     6601 ggagggaaca gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc
     6661 ttggaaaacc cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt
     6721 ggcccggaaa tgacggcgct gcgtgatggt gtgcgcggga aagataagct ggatgtgccg
     6781 atcaaaatga tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac
     6841 cgtacccatg aaatcctcca ggatgataag aagtgcgagc tgattgtggt tatcgactgc
     6901 cacatgacct catcggcgaa atatgctgac atcctgctgc ctgactgcac cgcttccgaa
     6961 cagatggact ttgcactgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat
     7021 caggtgatta aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca
     7081 aaacgtcttg gcgttgagca acagtttact gaaggccgta cccaggaaga gtggatgcgg
     7141 catctgtatg cccagtcgcg ggaagcgatt cctgaactgc caacgtttga agagttccgc
     7201 aagcagggga tctttaaaaa gcgcgaccca caagggcatc acgttgctta taaagccttc
     7261 cgtgaagatc cgcaggcaaa cccactgact acgccatcgg gcaaaattga gatttattcg
     7321 caggcgctgg ctgacattgc cgctacctgg gaattgccag aaggcgatgt gatcgatcca
     7381 ctgccgatct acacgccggg ctttgaaagt tatcaggatc cgctgaacaa acagtatccg
     7441 ctgcagctta caggtttcca ctataaatct cgcgttcact caacttacgg caacgttgat
     7501 gtgctgaaag cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc
     7561 ggtatccaca acggcgataa agtcaggatc tttaacgatc gtggtgaggt tcatattgag
     7621 gcgaaagtga cgccacgaat gatgccgggc gtggtcgcac tgggtgaagg cgcctggtat
     7681 gacccggatg caaaacgtgt cgataagggc ggttgtatca acgtactgac cactcaacgt
     7741 ccgtctcctc tcgctaaggg gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg
     7801 taaggagtaa ccgatgacaa cccagtatgg attttttatt gattccagcc gttgcaccgg
     7861 ttgcaaaacc tgcgagctgg cttgtaaaga ctacaaagat ttgacgccag aagtcagttt
     7921 ccgccgcatt tatgaatatg ctggcggcga ctggcaggaa gataacggtg tctggcacca
     7981 gaacgtgttt gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac
     8041 taaagtctgc ccgagcggtg cgatgcataa acgcgaagat ggttttgttg tggtcgatga
     8101 agatgtatgc attggctgcc gttactgtca tatggcttgc ccgtatggcg caccgcaata
     8161 taacgaaacg aaaggccata tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg
     8221 taaaaagccg atctgtgttg aatcttgccc gctgcgggcg ctggatttcg gccctatcga
     8281 cgagctgcgt aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt
     8341 taccaaaccg aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac
     8401 tggctatctg gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc
     8461 tgatgatctt cacggtcttc gggcaatgtg tggcgggtgg ttttatcgtt ctggctttgg
     8521 cgctgctcaa aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg
     8581 gcttatgggt actgatgggc attggcttta tcgcctctat gctccatctt ggttcaccga
     8641 tgcgcgcctt taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca
     8701 gcggttcgat cttttttgct gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc
     8761 tgtcaccggc attgcgtacg ctatggctgg tagtgacgat ggttcttggc gtcgtctttg
     8821 tctggatgat ggtgcgtgtg tataacagca ttgataccgt tccgacctgg tacagcatct
     8881 ggacgccgat gggcttcttc ctgacgatgt tcatgggcgg cccgttactg ggttacctgt
     8941 tgctgagtct ggctggcgtc gatggctggg cgatgcgtct gctgccagcc atttctgtac
     9001 tggcactggt ggtgagtggc gtgatgtcag tgatgcaggg cacagagctg gcaactattc
     9061 atagttctgt gcagcaggcc gcagcgctgg tgccggacta tggtgcgctg atgtcctggc
     9121 ggatcgtgct tttggccgtt gccctgtgct tgtggattgc accacagcta aaaggttatc
     9181 agcctgcggt tccgctacta tccgtctcat tcattctgct tctggcaggg gaattaatcg
     9241 gtcgcggcgt attctatggt ttgcatatga ccgtggggat ggccgtcgca agctaatcag
     9301 taacaaccgg ggtttcggcc ccggttctct tttatttctg cttcgttaac gtgtcataac
     9361 tggtcatcaa attacgataa tcaggaatat ggttagagaa caacgtcgcc agtccttcaa
     9421 tatcattacg ccagtcacgg tgcagctcgc aagccacgcc gaaccatgtc atcaattgcg
     9481 caccagcttg cgacatgcga tcccacgccg aatgccgggt aatttcatta aaggtccctg
     9541 atgcatcagt cacgacaaag acatcaaacc cttcttcaat ggctgaaagc gccgggaatg
     9601 ccacgcaaac ttcggttacc acaccggcaa taattaactg ttttttacct gtcgctttga
     9661 ctgcttttac aaaatcttcg ttatcccagg cgttaatatt gcccgggcga gcaatgtaag
     9721 gtgtatcggg gaattgtgct ttcagttctg gaactaatgg gccgttagga ccggtttcaa
     9781 aactggtggt aagaatagtt ggtaagttga aatacttagc caggtcaccc agcgccagca
     9841 cgttattttt aaacttatcg ggttcgatat cccgtacaag ggaaagtaaa ccagcctggt
     9901 gatcaacaag caaaacggca gcatcatttt tatcaagacg aacatacggt ttggtcatcc
     9961 tcttctcctt tcgagagtca gtggtacgtc tgaaatcggt cagacgcgat gcattgctct
    10021 gaaagcatag acgggaaata tgagtttgct gtgaccatga aatttttcga ctgaacgaag
    10081 cgtatgaaat ttgtgttaga tcaataaaaa caatcagatg agattaatca tctcttcagc
    10141 attagcgtta tgaatgagta atataaatcg ataataccgc cgctgcgata tgcgcgtatt
    10201 ttcacccatt gacaatgttt ttggcggtgg catgatgcgc atgaaatttg aacttcctca
    10261 cggttttaat tcatgtccac gtatacccgg cctgtcatgc ttttgctgtc tggcctgctt
    10321 ttgttgactc tggcgatcgc ggtgttaaat acactcgtgc cgctttggct cgcccaggaa
    10381 cacatgtcca catggcaggt aggcgttgtc agctcatcct attttaccgg caaccttgtc
    10441 ggtacattgc tgacagggta tgtcattaaa cgcattggct ttaaccgcag ctattatctg
    10501 tcctccttca tttttgctgc tggctgtgcc ggccttggcc tgatgattgg tttctggagc
    10561 tggttggcgt ggcgttttgt cgcgggcgtc ggctgtgcca tgatttgggt ggttgttgag
    10621 agcgcgctga tgtgcagtgg gacgtcacgt aaccgtgggc gtttgcttgc tgcgtatatg
    10681 atggtttatt acgtgggaac gtttttaggc cagttactgg tcagcaaagt ctcaaccgag
    10741 ctgatgagcg tattgccgtg ggttacaggt ttgacattgg cagggatctt accgctgttg
    10801 tttacgcgtg tcctgaatca gcaggctgaa aaccatgatt cgacgtcaat tacggcaatg
    10861 ctaaaactcc gtcaggcgcg gcttggcgtg aatggctgca ttatctcagg aatcgttctg
    10921 ggatctctat atggcctgat gcctctgtac ctcaatcaca aaggggtgag caattccagt
    10981 attggtttct ggatggcggt actggtcagt gcgggtatcc tcggacaatg gccgattgga
    11041 cgtctggcgg ataagtttgg tcgactgctg gttttgcgtg ttcaggtctt tgtcgtcatt
    11101 ctcggcagta ttgcgatgct tagccaggcg gcgatggccc cagcgttatt catcctcggt
    11161 gccgctggct ttacgctata tccggtggcg atggcctggg cttgtgagaa agttgaacat
    11221 catcaactgg tggcgatgaa ccaagcctta ctgttgagct ataccgtggg aagtctgctt
    11281 ggcccgtcat ttaccgctat gctaatgcag aatttctccg ataatttatt gtttatcatg
    11341 atcgccagcg tatcgtttat ctatttgctg atgctgcttc gcaacgccgg tcatacgccg
    11401 aaacccgttg ctcacgtgta aatgaattca agcaaagtgt gaacttactg tttcacactc
    11461 tgcttttttg tttcttctat ctgacttgct tttttccaaa ttttattcgt ttaaaaataa
    11521 aatgtgcagc aggttataat tttgcatttc gctattaccg cacttcttat ttgccgcgca
    11581 taatccctcg ttttacagat gcccctttaa ttttggcgaa ggatttgtct atggctggga
    11641 atgttcagga aaaacagttg cgatgg
//
LOCUS       DADLSB010000119        11582 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.119, whole genome shotgun sequence.
ACCESSION   DADLSB010000119 DADLSB010000000
VERSION     DADLSB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.119"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1176
                     /locus_tag="JN331_003038"
     CDS             <1..1176
                     /locus_tag="JN331_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261124.1"
                     /translation="RLNQQHNEFMMVAKWCEWAIQQDGLQENLQKNLYEILEENQQNK
                     QSEIPQEDLKESLEEIKENILEENLPASRIENRAEALRRMKECLITRRSMLNLSNLGL
                     TSLPENLPPHLIEFYCSKNVLTALPKVMPKWLLVLDCTDNVLILLPKVQPSKLMVLKC
                     YENCIIWLPELSTNLRVINCSENFLQFLPPSMPQYLYKLSCAGNNINSIPDEMLENLT
                     RLKVFDCSSNDLISSPRLPPKLIIYYCGENKFKTVQVPQPQSLKVFDCNGNPWDKDNL
                     PTLLKAVEGLKKQQGLKDLLDFLHKEGQVDLEGLEELEDLMDLEFLDDPELLERVKVQ
                     EDLELLDQQLGLLSLERQQDSQPVNQQSEHEPESASRVKRDLSEVDSESTMKRKRFM"
     gene            complement(1344..2414)
                     /gene="dadX"
                     /locus_tag="JN331_003039"
     CDS             complement(1344..2414)
                     /gene="dadX"
                     /locus_tag="JN331_003039"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY7261125.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     LRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(2424..3722)
                     /locus_tag="JN331_003040"
     CDS             complement(2424..3722)
                     /locus_tag="JN331_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY7261126.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLVRMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFCDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            4052..5584
                     /locus_tag="JN331_003041"
     CDS             4052..5584
                     /locus_tag="JN331_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY7261127.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFLPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVQDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(5636..6355)
                     /gene="fadR"
                     /locus_tag="JN331_003042"
     CDS             complement(5636..6355)
                     /gene="fadR"
                     /locus_tag="JN331_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY7261128.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            6576..8117
                     /gene="nhaB"
                     /locus_tag="JN331_003043"
     CDS             6576..8117
                     /gene="nhaB"
                     /locus_tag="JN331_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY7261129.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            8263..8793
                     /gene="dsbB"
                     /locus_tag="JN331_003044"
     CDS             8263..8793
                     /gene="dsbB"
                     /locus_tag="JN331_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY7261130.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(8839..10107)
                     /locus_tag="JN331_003045"
     CDS             complement(8839..10107)
                     /locus_tag="JN331_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY7261131.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMFSPRYTTRSSDLLRVK"
     gene            complement(10107..10526)
                     /gene="umuD"
                     /locus_tag="JN331_003046"
     CDS             complement(10107..10526)
                     /gene="umuD"
                     /locus_tag="JN331_003046"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7261132.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            10899..11582
                     /gene="hlyE"
                     /locus_tag="JN331_003047"
                     /pseudo
     CDS             10899..11582
                     /gene="hlyE"
                     /locus_tag="JN331_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT         2898 a         2976 c         2822 g         2886 t
ORIGIN      
        1 cgcctgaatc agcaacataa cgagttcatg atggtcgcca aatggtgcga atgggctata
       61 cagcaggatg gtttgcaaga aaacctgcaa aaaaacctgt acgaaatcct ggaagaaaac
      121 cagcaaaaca aacagtcaga aattccccaa gaagacctga aagaaagtct ggaagaaatc
      181 aaggaaaata tcctggaaga aaacctgcca gcaagccgga ttgaaaaccg ggcagaagca
      241 ttgcgcagaa tgaaggaatg tctcattaca agacggagta tgctcaatct tagcaacctg
      301 ggattgactt cactccctga gaatttgcct ccacatctga ttgaatttta ctgcagtaaa
      361 aacgtgttga ccgcgttacc gaaggtgatg ccaaagtggc tgctggtgct tgactgtaca
      421 gataacgtct tgatcttgtt accgaaggtg cagccctcaa aactgatggt actgaagtgc
      481 tatgaaaact gtattatctg gctgcctgag ctgtcgacta acctgagagt gattaactgt
      541 tctgaaaact tcttgcaatt tttaccgccg tcgatgcccc agtacctgta taaactatcc
      601 tgtgccggca acaatattaa ctcaatacct gatgagatgc tggagaactt gactcgcctg
      661 aaagtatttg actgttctag taacgatttg atctcttcac cacggctgcc gcccaaacta
      721 ataatttact actgtggaga aaacaagttt aaaacggtac aggtgccgca gccccagagc
      781 ctgaaggtgt ttgactgtaa tggtaacccg tgggacaaag acaatttacc gacgctgctc
      841 aaagccgtcg agggcctgaa aaaacagcag ggcctgaagg accttttgga ctttttgcac
      901 aaggaaggcc aggttgacct ggaaggactc gaggagctgg aggaccttat ggatctggag
      961 ttcctggatg acccggaact cctggagcgc gtgaaggtac aggaggacct ggagctcctg
     1021 gatcaacagt tgggcctgtt gagtctggaa agacaacagg actcgcagcc tgttaatcaa
     1081 caatctgaac atgaacccga atctgcatca agggtgaagc gtgatttatc tgaagtcgac
     1141 tccgagtcaa caatgaagcg taagcgtttt atgtagaacg cctgtgcggt gagttcaggt
     1201 tccggggaaa tagtgtccat tcttttaatg gacactatca tgggcaatgg ctctgtcaca
     1261 ttaacaacta cagttgctga ccagccggat ggatgatttt ttcgcaacca gaagacgctg
     1321 cctccgatcc ggcttacaac aagttacact gtcacaaccg ggacgcgtaa cgccagcgcg
     1381 cacatcaact catagcccac cgttccggca gcggcggcga catcatcaat tttgatctcc
     1441 ttgccccaca gctcaaccgg cgtaccaatc cccgcctgcg ggcaaggcgt taaatcaacc
     1501 gccagcatat ccatcgagac ggtccccacc gtcatggtgc gcacgccgtc cactaaaaca
     1561 ggagtaccgg taggcgcatg gcgcagataa ccgtcggcgt atcctgcggc gacaatgccg
     1621 attcgctgtt catcgcgcgc agtatagcga ccgccgtagc ccacacgctc gcccgctttt
     1681 agcgtctgga caccaataat ctcactgctt agcgtcatca ccggacgtaa tccggtattg
     1741 gcgatatcac gccactgacc ggacggcgaa gcgccataca aaataatgcc aggccgaacc
     1801 cagtcaaaat gcgcttccgg gtgccacaga gtcgccgccg aattggacaa cgaacgccga
     1861 cactccagcc cctccgccgc ctgctcaata cgcgccatcg cgccggaaat tccatcagga
     1921 tgttccgctt cagcaaaatg cgacatcagg gtcatttcgc caacattcgc cattgcccgc
     1981 aactgctgcc agacggtaag cacgcgatcg ggctggaagc ccaaccgatt catcccactg
     2041 ttcactttaa gataaatatc caacggtgct tttagccgcg cattttgcag tgctttgagc
     2101 tgccagttgc tgtgtacgca ggtggtcagg cggtgctggt cataaatctc cagatcctga
     2161 gcatggaaaa atccttccag catcaggatc ggccccttcc agccgcgctc acgtaacgtt
     2221 attgcctctt ccaggttaag taatgcaaag ccatcggtgg ccccgagcgc gctccagata
     2281 cgctcaatac catgcccgta agcgttcgct tttaccaccg accagacgcg cgcgtgcggc
     2341 gcggcctggc ggacaatgga cagattctgt tttaatgcct gcagatcgag gctggcctgt
     2401 atcggacggg tcatctcgtt tccttagctg tgtgcgccat gtaaatggcc cggacgtgat
     2461 ggcgtaaatc cacggctgta gcgcgctacg cttagatcct catatgggat cgctggcgtg
     2521 cgaccagaga gcagatcgct taacaactga ccggaaccgc aagccatcgt ccagccgagc
     2581 gtgccgtgac cggtattcag ccacagattt ttaaagcgtg tacgcccgac aaccggcgta
     2641 ccgtctggcg tcatcgggcg caaacccgtc cagaaagtcg cctgctcgac atgaccgccg
     2701 cgtggataga gatcgcgaac caccatttcc agcgtttcac gacgcggttg caacagctcg
     2761 gtgttaaaac caacaatttc cgccattccg ccaacacgaa tgcggttatc gaaacgagta
     2821 atggcgattt tgtaggtttc atcaagaatg gtggatactg gcgcaccatc ttcctgcgca
     2881 attggaatag tcagcgagta gcctttcagc ggatacaccg gaatatcaac aatgcctttg
     2941 agcatcgccg tcgagtaaga accaaacgcc atcacatacg catcggcctt aatcacttca
     3001 tcgcaaaact tcacgccgta gatttgctcg ccgtcgcaaa gcagttggtc aacgggcgta
     3061 ttaaagcgga atttaacccc cgcctgctct gccatccgca ccagattctg ggtaaacagc
     3121 tggcagtctc cggtttcatc attgggtaac tgcaggccgc ccgtcagttt gtgcgctact
     3181 tctgccagcg cgggatccac ttccgccagg cggctggatt ccagcagctg atacggtacg
     3241 ccggcatctt ccagcacggc gatatcgcgg gtcgcatttt catactgttg ttcggtacgg
     3301 aacagttgca gcgtcccacc ctgacgccct tcatactgaa tattggtttc ggcgcgcaat
     3361 gctttcaggc aatcacggct gtattccgcc agacgcacca tccgcccttt gttttccatg
     3421 tagtggctgg tgtcgcagtt acgtaacatt tgccacatcc atttcaactg aaactgcgta
     3481 ccgtcgagac gaaccgccag cggcgcatgg cgctggaaca tccatttaat cgcttttaaa
     3541 ggcacacctg gtgctgccca cggtgcagca tatccggggg agatttgccc ggcattcgcg
     3601 gcactggttt ccagggcggc ccccggctcc cgatcaatga cggtgacctc atgtcctgcc
     3661 tgatttaagt accaggcgct ggcaacgcct accacaccac ttcccagtat gacaactcgc
     3721 atagccactc cgataacggt agatacagta aaagaataat aatctaatta catcttgata
     3781 actcagttga aaatattatt caacatacgg cttttttatg gtgagctgac tcacatctcc
     3841 ctgactctgc ggcaatatgg cgtctttagt atctcctgct gaacgccctt tttatccagc
     3901 ataaaattcc gttcagaagc ggattagtgg cactctgata ataagaaatc gcaaagaaaa
     3961 aaattctccg gcgacgcggt ttttaacagg tgttctatgc ttgaaatgag gtgctccaca
     4021 cagagagcgc cgacaacaat gaggtgcgcg tatggcgacg atcgattcta tgaataagga
     4081 caccacacgt ttgagcgatg gacccgactg gacgttcgac ctgctggatg tttatctggc
     4141 agagatagac cgggtggcga aactctaccg gctggatacc tacccgcacc agattgaagt
     4201 gataacctca gaacagatga tggatgccta ttccagcgtc ggcatgccaa ttaactatcc
     4261 gcactggtca ttcggtaaaa agtttatcga gactgaacgg ctgtataagc acggtcagca
     4321 agggctggcc tatgaaatcg tcattaactc taacccgtgt atcgcttact tgatggaaga
     4381 gaacaccatt accatgcagg cgctggtgat ggcacacgcc tgctatgggc ataactcttt
     4441 ctttaaaaac aattgcttat tccgcagttg gaccgacgcc agttcgattg tcgattatct
     4501 gatttttgcc cgtaaatata ttaccgagtg cgaagaacgt tatggcgttg atgaagtaga
     4561 acggcttctg gactcgtgcc acgcgctgat gaactacggc gtggaccggt acaaacgccc
     4621 gcaaaaaatc tcgctgcaag aagagaaagc ccggcagaaa agtcgcgaag agtatctaca
     4681 aagtcaggtc aatatgctct ggcgtaccct gccgaagcgc gaggaagaga aaacggttgc
     4741 tgaagcgcgc cgctatccgt ccgaaccaca agaaaacctg ctctatttta tggagaaaaa
     4801 tgcgccactg ctggaatcat ggcagcgtga aatcctgcgt attgtgcgta aggtgagcca
     4861 gtatttttat ccgcaaaaac agactcaggt gatgaacgaa ggctgggcga ccttctggca
     4921 ctataccatc cttaaccatc tgtatgatga agggaaagta acggaacgtt ttatgctgga
     4981 gtttttgcac agccacacca atgtggtctt cctgcccccc tataacagcc cgtggtacag
     5041 cggcatcaac ccgtatgccc tcgggttcgc catgttccag gatattaaac ggatttgtca
     5101 gtcgccaacg gaagaagaca aatactggtt cccggatatc gccggttccg actggctgga
     5161 aacgctgcat ttcgcgatgc gtgatttcaa agatgagagt tttatcagcc agtttctgtc
     5221 accgaaagtg atgcgtgatt tccgcttctt caccgtgcag gatgatgatc ggcataatta
     5281 tctggagatt tccgctattc ataatgaaga aggttatcgg gagatccgta accggttatc
     5341 gtcgcaatat aacttaagta atctggagcc gaatattcag atctggaacg tggatttgcg
     5401 cggcgaccgt tcgctgacgc tgcgttatat tccacataat cgcgcaccgc tggatcgggg
     5461 gcgcaaagaa gtcctgaagc atgtgcatcg cctgtgggga tttgatgtga tgctcgaaca
     5521 gcaaaacgaa gacggcagca tcgagttgct ggaacgttgc ccgccaagaa tgggaaatct
     5581 gtaaaaacaa caaaaaaccc ctcgtttgag gggtttgctc tttaaacgga agggattatc
     5641 gcccctgaat ggctaaatca cccggcagat ttttctgcat ccggtgccaa atctcgccac
     5701 tctcatgccc atagcgacgc actgtttcgt acacctgatc gtgcgcgcct tcactgcaca
     5761 acgccgacag tttgtggtag aagcccagcg ccagactgcg cgcttccgga ttggcgaaat
     5821 agtgacgacc aatgcgcgta tacagccctt tcatcccgtt aaggatcaga ccgtaaatcg
     5881 ggttgccgga agcaaacgcc aggccgcgga atatgttgta atccagctcg gcaaaggcat
     5941 cggcgtgatc ggccacttca ttagcggtag ccagcacttc ctgcgcttta tcgggatgct
     6001 gacgaaacgc ggtgcgaata aaaatagtgg aaatattggt acgcaccgac agcaaattat
     6061 caataagctg cggcacactt tcgtgatcca gtcgcgccag tgtttcaagg atatttaaac
     6121 cggaagtttc ccagaaatta ttcaccttcg tcggcttgcc atgttgaatg gtcaaccagc
     6181 catctcgtgc cagacgctgt aacacttcac gtaacgtagt acgcgttacg ccaattaatt
     6241 ctgaaagttc acgttctgcg ggcaaaatag tcccgggagg gaagcggtta ttccagatac
     6301 tttcaataat gtactcttcc gcgaaacccg ccgggctttg cgccttaatg accatagtga
     6361 gatttccata acacagcaaa acaaagttgg actcatcata ccagagggct aactacgctg
     6421 atagcagaca aaaagaagaa aaagggatca aggcttcatt ttccgcatct ctttcccctt
     6481 gactatcaag acttgcctca ctcctgcctg accgttaagc tttgcgttta aacataaaaa
     6541 catcattgat tatttgtaag gtaagggaaa ccatcatgga gatctcctgg ggccgcgcgc
     6601 tatggcgcaa ttttttgggc cagtcccccg actggtacaa actcgccctc atcattttct
     6661 taatcgtaaa cccgttaatt ttcctcatca gccctttcgt cgcgggttgg ttgctggtag
     6721 cggaatttat tttcactctg gcgatggccc tgaaatgcta cccgctactc cccggtggtc
     6781 tgttggctat cgaagcggta ttcatcggca tgaccagcgc ggaacacgtc cgtgaagagg
     6841 tggcggcaaa tcttgaagtc ttgctgttat tgatgtttat ggtggcgggt atctatttta
     6901 tgaaacagct gttgctgttc atatttaccc gtttgctgtt aagcattcgc tccaaaatgc
     6961 tgctgtcgct ctctttttgc gtggcggcag cgttcctctc cgcgttcctc gatgccttaa
     7021 ccgtcgtggc ggtggtgatc agcgttgcag tcggttttta tggtatttat catcgcgtag
     7081 cctcttcccg taccgaagac accgacctgc aagacgatag tcatatcgac aagcattaca
     7141 aagtggttct ggaacagttc cgtggctttc tgcgtagcct gatgatgcat gccggtgtcg
     7201 gcaccgcatt aggcggcgta atgaccatgg tgggcgaacc acagaacctg atcatcgcta
     7261 aagcggctgg ctggcatttt ggcgatttct tcctgcgcat gtcgccggtg accgttccgg
     7321 ttctgatttg tggcctgtta acctgcctgc tggtagagaa gctgcgttgg tttggctacg
     7381 gtgaaacgct gccggagaaa gtccgcgaag tgttgcaaca gtttgacgat caaagccgcc
     7441 accagcgtac ccgtcaggat aaaatccgtc tgattgtcca ggcgattatt ggcgtctggc
     7501 tggtgactgc gctggcgttg catctggcgg aagttggctt gattggtttg tcagtcatta
     7561 ttctggcaac atcattgacc ggtgtcaccg atgaacatgc tatcggtaaa gccttcaccg
     7621 aatctctgcc attcaccgca ctgttgacgg tgtttttctc ggtagtcgcg gtgattatcg
     7681 accaacaact gttttcgccg attattcagt ttgtgttgca ggcatctgaa catgctcagc
     7741 tgtcgctgtt ctatattttc aacggtctgc tgtcatccat ttcggataac gtcttcgtgg
     7801 ggacgattta tatcaacgaa gcgaaagcgg caatggaaag tggcgctatc acgttgaagc
     7861 aatacgagct gctggcggtc gccattaata ctggtaccaa tctgccctct gtcgctacgc
     7921 cgaacggtca ggctgcgttc ctgttcctgc tgacctctgc actcgcgcca ttgattcgcc
     7981 tctcttatgg ccgcatggtg tggatggccc tgccttacac cctcgtactg acactcgtcg
     8041 gcttgctctg cgtcgagttt acgcttgccc ctgtaaccga atggtttatg caaatgggct
     8101 ggatagcaac gctttgataa caacttaccg ggcatataaa tgcccggttt gccttttcgc
     8161 ccgataattg tccaattgcg tcctattttt gctgcctcct ggtggcgcag cgaatgaatt
     8221 ggtttaaact gcgcactcta tgcatattgc agggaaatga ttatgttgcg atttttgaac
     8281 caatgttcac aaggccgggg cgcgtggctg ttgatggcgt ttactgctct ggcactggaa
     8341 ctgacggcgc tgtggttcca gcatgtgatg ttactgaaac cttgcgtgct ctgtatttat
     8401 gaacgctgcg cgttattcgg cgttctgggt gctgcgctga ttggcgcgat cgccccgaaa
     8461 actccgctgc gttatgtagc gatggttatc tggttgtata gtgcgttccg cggtgtgcag
     8521 ttaacttacg agcacaccat gcttcagctc tatccttcgc catttgccac ctgtgatttt
     8581 atggttcgtt tcccggaatg gctgccgctg gataaatggg tgccgcaagt gtttgtcgcc
     8641 tctggcgatt gcgccgagcg tcagtgggat tttttaggta tggaaatgcc gcagtggctg
     8701 ctcggtattt ttatcgctta cctgattgtc gcggtgctgg tggtgatttc ccagccgttt
     8761 aaagcgaaaa aacgtgatct gttcggtcgc taatccattc ggcgctcctg cgggagcgct
     8821 tttttcctgc cgctatattt atttgacccg cagtaaatca gaacttcgcg ttgtataacg
     8881 tggtgaaaac atagctcgct tcatctgcca ttgttgttgg atcccctgcc cggcaaaata
     8941 gagtgttcct ctgccctctt tagcattcag agtatccatt accgccatca attgctcact
     9001 cccggggcgc ggtgcgttgt catcgaataa attgagctgc gcgactccct gactgaagaa
     9061 atcccccagc atcacgcccg ctttttggta gcgatggccc gcttgccaga tggcatccag
     9121 agatcgcgta gcagcgttaa tgatatccct gctgtcctga gtgggcgtca gcagttttac
     9181 cgacgcgcta ttgccgtaat aaggttcatt gagcgcaaat ggtgacgtct taataaatgt
     9241 ggagataaac cgacaatatt gatgctcact gcgaagtttt tccgccgccc gggcagcgta
     9301 actacaaatg gcctgccgca tcgacgtata atccgtgatg cgttcaccaa acgagcggga
     9361 acagataatt tcctgcttcg tcggtgcaaa ctcttccagt tgcaaacagg gttcgccgcg
     9421 cagttcacgc accgttcttt cgagcatgac attaaaatgt ttacggataa accggatatc
     9481 tgtatccgcc aaatcgagaa cggttttgat ccccatcgcg tccagttttt tgctgatccg
     9541 ccgtccaatc ccccagacgt catccacggg gagagcagac attaatttac gctggcgttc
     9601 cagatttgat aaatccacca ccccacccgt ctgccgctgc catttttttg ccgcatgatt
     9661 ggcaagctta gccaacgttt tggtctgggc gatccccaca ccaacagtaa gatgggtacg
     9721 ttgtagcacc gttgcgcgaa tttctctgcc aaaatcagtc agatcgcgac aattacgcac
     9781 acctgtcaga tcgcagaatg cttcatcaat actgtaaatc tcgacgcggg gcgatagttc
     9841 ttccagcgtc gacatcaccc gattgctcat gtctgcgtaa agctcataat tgctgctaaa
     9901 gcaaaccaca ccacagcgac gaaacagatc tttttgtttg aaccagggat cgcccatttt
     9961 aacgcccagc gcctttgcct cagcgtttcg ggcgataacg caaccgtcat tattcgatag
    10021 cacaaccacc ggtttacccc ataaatcagg gcgaaacacc gtctcacagc tggcataaaa
    10081 cgcgtttaca tcacagaggg caaacatcag cgcatcgcct taacgacgtg gatcaccaca
    10141 ccaaagacat ccagcgtatc ttcgctactg atggtaatgg gcgagtacgc actgttcatg
    10201 ggaataagct gtaccgtcgg gcgtagttgc aattttttca ccgtaaactc gccgtcaaca
    10261 gcagcgatga caatatcacc atggctggcg gtaatagcgc tatcgacaat cagtaaatca
    10321 ccgtcactaa ttccaccatc aatcatagaa tcaccacttg ctttgacgaa gtaagtcgcg
    10381 ctgggatgct ggatcaacag ttgattcaga tcgatgcgct gttcaacgta atctgctgcc
    10441 ggtgaaggaa agccacactg aacaagatcg ctaaatagcg gaaaagtcac aatttcgcgg
    10501 agatccgcag gcttgataaa caacataata atctgcctga agttatactg tttttatata
    10561 cagtagtctg ttcttgccag cagatcaata ctgattcagg ctatcaatat ttgtcgctgc
    10621 atagactgct gatttttcgt tctcttatcc tgtgctcacg tggccttctg gcgacgacgc
    10681 tcatccagca gaaatgaaaa atatcacccg gcaaaaaaat agaatagaag cattcgccat
    10741 aatgacatta aacattgttt gatatttatc atattaatag aaataaagac attgacgcat
    10801 cccgcccggc taactatgaa ttagatgaag taaaatttat taatagttgt aaaacaggag
    10861 tttcattaca atttatatat ttaaagaggc gaatgattat gactgaaatc gttgcagata
    10921 aaacggtaga agtagttaaa aacgcaatcg aaaccgcaga tggagcatta gatctttata
    10981 ataaatatct cgatcaggtc atcccctggc agacctttga tgaaaccata aaagagttaa
    11041 gtcgctttaa acaggagtat tcacaggcag cctccgtttt agtcggcgat attaaaacct
    11101 tacttatgga tagccaggat aagtattttg aagcaaccca aacagtgtat gaatggtgtg
    11161 gtgttgcgac gcaattgctc gcagcgtata ttttgctatt tgatgagtac aatgagaaga
    11221 aagcatccgc ccctcattaa ggtactggat gacggcatca cgaagctgaa tgaagcgcaa
    11281 aaatccctgc tggtaagctc acaaagtttc aacaacgctt ccgggaaact gctggcgtta
    11341 gatagccagt taaccaatga tttttcagaa aaaagcagct atttccagtc acaggtagat
    11401 aaaatcagga aggaagcgta tgccggtgcc gcagccggtg tcgtcgccgg tccatttggt
    11461 ttaatcattt cctattctat tgctgcgggc gtagttgaag ggaaactgat tccagaattg
    11521 aagaacaagt taaaatctgt gcagagtttc tttaccaccc tgtctaacac ggttaaacaa
    11581 gc
//
LOCUS       DADLSB010000120        11541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.120, whole genome shotgun sequence.
ACCESSION   DADLSB010000120 DADLSB010000000
VERSION     DADLSB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.120"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(520..1860)
                     /gene="fadL"
                     /locus_tag="JN331_003048"
     CDS             complement(520..1860)
                     /gene="fadL"
                     /locus_tag="JN331_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY7261133.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            2232..2516
                     /locus_tag="JN331_003049"
     CDS             2232..2516
                     /locus_tag="JN331_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY7261134.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2696..4006
                     /gene="fadI"
                     /locus_tag="JN331_003050"
     CDS             2696..4006
                     /gene="fadI"
                     /locus_tag="JN331_003050"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY7261135.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVLLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            4006..6150
                     /gene="fadJ"
                     /locus_tag="JN331_003051"
     CDS             4006..6150
                     /gene="fadJ"
                     /locus_tag="JN331_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY7261136.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPFIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6353..6838
                     /gene="sixA"
                     /locus_tag="JN331_003052"
     CDS             6353..6838
                     /gene="sixA"
                     /locus_tag="JN331_003052"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY7261137.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7521..8084
                     /locus_tag="JN331_003053"
     CDS             7521..8084
                     /locus_tag="JN331_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7261138.1"
                     /translation="MSKFVKTAIAVAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            8166..10807
                     /gene="yfcU"
                     /locus_tag="JN331_003054"
                     /pseudo
     CDS             8166..10807
                     /gene="yfcU"
                     /locus_tag="JN331_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            10827..>11541
                     /locus_tag="JN331_003055"
     CDS             10827..>11541
                     /locus_tag="JN331_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7261139.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGN"
BASE COUNT         2956 a         2675 c         2968 g         2942 t
ORIGIN      
        1 aagtagatgt ttaatacagg tgcttattcc ttccttacca ttaaaaatgg tttttgaact
       61 cttgtactaa tccattaatt tcatcattgt tattttcatt gatggattct gggtaagctg
      121 cgctggtatt gttgattgaa atagatggta gcatgatttt attccatgtt gattgatttg
      181 ttacaaatat atattaccta atgaaataga ctatcaaaaa acgctcattt atttagaacc
      241 tatctttaat ttgagtgtat aagcgcacaa tagccagggg ggttgagtat cctggaaatg
      301 cagaaaagta aactctttga gaaggacgag cagaacgctt gaaaatatgc atttcccttc
      361 aggtttgtct gtggatacca cttactgatt tgaaagggca agctgccatc aggctgtttg
      421 caaaactcag gaggcaagca atgaggaaac tactttcggt gaagtggggg agttaaagtc
      481 acctgcatag caggtgactt tatccaggcg aacgcgttat cagaacgcgt agttaaagtt
      541 agtaccgaac agccaggctt taccttcaga ctcgaactgg tatgggcctt cgttaatttt
      601 cacgctctga ccgtgcatat aagaaacacc aacgtcgact gaagcatctt tattaaatgc
      661 gtaagtcgta cctgcactca gccagaaacg gtcctggtcc ggaatggaga tagaacgatt
      721 ctgagccgga actgggctgt catcaaaggc gataccggta cggaaggtcc agttatcatc
      781 gtagtaataa gtggtaccca acgcgatgcg gtaagcatct ttaaagcctt catgtttctg
      841 gaacagcgtg tcgccactgg ttgaggtcgc tttcagctgc tggaactgac tccagctggt
      901 gtaagccagg ctatagtgaa tcgcccactg tggatcaaca cggttataac ctgacacttc
      961 ccacatttca ggcaggttca gcgtcagata acccgattgc gttgcgccac ctgtcgcggt
     1021 aggaattggc aaaccgtagt tattaaacgc acgattaaga tcgctgctgt agttaccttt
     1081 gaagtcaatt ttcacttcag aacggtaggt cagtgcatag cggttatttt tatccagttc
     1141 atacaggatt ccggcgttcc agccaaagcc ccactggtta ccgttcagat gagcgatttt
     1201 ggtattactg tcgataccgt tggcggtagc tgccaatgct tgcccttgag gagtttgccc
     1261 ggcaggagat tgcataatct gaccagcaac caactgcccc agatcgcctg cgaaacgttc
     1321 aattttcgcg cgagcgtaga tggcgttgaa accaagacca aagctccatg cattatttaa
     1381 gcgatacgca ccgcttaagt tcaggttcat ggtttcaagg tcagttgtac ccccgacaga
     1441 gccgcctgca taagtatcat taaactctgt tgccagacca tagttagagg taatagaagc
     1501 gccccaacca aattggtcgt taatcggtgc aacaaagtgc atgttcggaa cccatgccgt
     1561 aggcgcgatg ttatcggctt tcaggctacg accagacgga gacgttccgc tgatattcac
     1621 atccgggtca atataaaccg cacccgcaga aaatgtcggg cggtcaaaca tggtaatcaa
     1681 tgcggggtta cggctaacgt tacctgcatc atcggcaatt gcgccttccc ctgaataagc
     1741 ccggcccagg ccagaggaag aaaattcgtt taactgaaag cctgccgacc aggcctgggt
     1801 ggagataagt gccactgcga ctgcgagagc agactttgta aacagggttt tctggctcat
     1861 gaccataacc tcaatgattt atttttatac aaactatgtt acgtgctgta acaggagcgc
     1921 gaagtgtagg gtctgaggta catcttagaa atcagaccag tggcgagagt ataggtcgga
     1981 ccagctagaa tattgcaagt atgtttccat cttttttcaa atatgatcta agaaacgcga
     2041 tccagctggc aaaatttgga ggttgcgcac tcgcggattc gggtgaattt tgttttagat
     2101 catttttaag tgtgatttcg gtcacttatc cgattttgca gaattaacaa ctggagcagc
     2161 actttccgga agcgtaaaat atccacaacg tttatctggc accttgggtg cacatacaga
     2221 ggaaatcaac gatgagtaaa tgtagtgctg atgaaacccc ggtttgctgc tgtatggatg
     2281 ttggcaccat tatggacaac tccgactgca ccgcgtctta cagccgcgtg tttgcaaacc
     2341 gcgcagaagc agaacaaacg ctggcggcgc tgactgaaaa agcccgtagc gtggaatccg
     2401 aaccgtgcaa aatctccccg actttcactg aagagtctga cggtgtgcgc ttggatatcg
     2461 atttcacttt tgcctgcgaa gctgaaatgc tgatcttcca gctgggtctg cgttaattcc
     2521 tctcgggtac gtacgcccct gtcagttgct ggcaggggcg ttttcttttc tttcttctgt
     2581 cttcactgtg tcatagctcc cactttcctt tttctccgct tggctaaatg taaaaaaatg
     2641 gttaagacta tgatcaggtc agaccacttt atttattttt ttacagggga gtgttatggg
     2701 tcaggtttta ccactggtta cccgccaggg cgatcgtatc gccattgtta gcggtttacg
     2761 tacgcctttt gcccgccagg cgacggcttt tcatggcatt cccgcggttg atttagggaa
     2821 gatggtggta ggcgaactgc tggcacgcag cgagatcccc gctgaagtga ttgaacagct
     2881 ggtctttggt caggtcgtac aaatgcctga agcccccaac attgcgcgtg aaattgttct
     2941 cggtaccgga atgaatgtgc ataccgatgc ttacagcgtc agccgcgctt gcgctaccag
     3001 tttccaggcg gttgcaaacg tcgcagaaag cctgatggcg ggaactatcc gagcggggat
     3061 tgccggtggg gcagattcct cttctgtatt gccaattggc gtcagtaaaa aactggcgca
     3121 cgtgctggtt gatgtcaaca aagctcgaac catgagccag cgcctgaaac tcttctctcg
     3181 cctgcgtttg cgcgacttaa tgcccgtacc gcctgcggta gcagaatatt ctactggctt
     3241 gcggatgggc gacaccgccg aacaaatggc gaaaacctac ggcatcaccc gagaacagca
     3301 agatgcatta gcgcaccgtt cgcatcagcg tgccgctcag gcatggtcag acggaaaact
     3361 caaagaagag gtgatgactg cctttatccc tccttataaa caaccgcttg tcgaagacaa
     3421 caatattcgc ggtaattcct cgcttgccga ttacgcaaag ctgcgcccgg cgtttgatcg
     3481 caaacacgga acggtaacgg cggcaaacag tacgccgctg accgatggcg cggcagcggt
     3541 gatcctgatg actgaatccc gggcgaaaga attagggctg gtgctgctgg ggtatctgcg
     3601 cagctacgca tttactgcga ttgatgtctg gcaggacatg ttgctcggtc cagcctggtc
     3661 aacaccgctg gcgctggaac gtgccggttt gacgatgagc gaactgacat tgatcgatat
     3721 gcacgaagcc tttgcagctc agacactggc gaatattcag ttgctgggta gtgaacgttt
     3781 tgctcgtgaa gtactggggc gtgcacatgc cactggcgaa gtggacgata gcaaatttaa
     3841 cgtgcttggc ggttcgattg cttacggaca tcccttcgcg gcgaccggcg cgcggatgat
     3901 tacccagaca ttgcatgaac ttcgccgtcg cggcggtgga tttggtttag ttaccgcctg
     3961 tgctgccggt gggcttggcg cggcaatggt tctggaggcg gaataatgga aatggcatca
     4021 gcgtttactc ttaatgttcg tctggacaac attgctatca tcaccatcga cgtaccggat
     4081 gagaaaatga ataccctgaa ggcggaattt gcctcgcagg tgcgcgccat tattaagcaa
     4141 ctccgtgaaa acaaagagtt gcgcggcgtg gtgtttatct ccgcgaaacc ggacaatttt
     4201 atcgccggag cagacatcaa catgattggc aactgcaaaa cggcgcaaga agcggaagca
     4261 ctggcgcggc agggccaaca gttgatggcg gagattcatg ctttgcccat tccggttatc
     4321 gcggctattc atggcgcttg cctgggcggt gggctggagc tggcgctggc gtgccacggt
     4381 cgcgtttgta ctgacgatcc taaaacggtg ctcggtttgc ctgaagtaca acttggattg
     4441 ttacccggtt caggcggcac ccagcgttta ccgcgtctga taggcgtcag tacagcatta
     4501 gagatgattc tcaccggaaa acaacttcgg gcgaaacagg cattaaagct ggggctggtg
     4561 gatgacgttg ttccgcactc cattttgctg gaagccgctg ttgagctggc gaagaaggat
     4621 cgcccatctt cccgccctct acctgtacgc gagcgtattc tggcggggcc gttaggtcgt
     4681 gcgctgctgt tcaaaatggt cggcaagaaa acagaacaca aaactcaagg caattatccg
     4741 gcgacagaac gcatcttgga ggttgttgaa acgggattag cgcagggcac cagcagcggc
     4801 tatgacgctg aagctcgcgc ctttggcgaa ctggcgatga cgccgcaatc gcaagctttg
     4861 cgtagtatct tttttgccag tacggacgtg aagaaagatc ccggtagtga tgcaccgcct
     4921 gcgccattaa acagcgtggg aattttaggt ggtggcttga tgggcggcgg tattgcttat
     4981 gtcactgctt gtaaagcggg gcttccggtc agaattaaag atatcaaccc gcagggcata
     5041 aatcatgcgc tgaagtacag ttgggatcag ctggagggca aagttcgccg tcgtcatctc
     5101 aaagccagcg aacgtgacaa acagctggca ttaatctccg gaacgacgga ctatcgcggc
     5161 tttgcccatc gcgatctgat tattgaagcg gtgtttgaaa atctcgaatt aaaacaacag
     5221 atggtggcgg aagtggagca aaattgcgcc gctcatacca tttttgcttc gaacacgtca
     5281 tctttaccga ttggtgatat cgccgctcac gccgcgcgac ctgagcaagt tattggcctg
     5341 catttcttca gtccagtgga aaaaatgccg ctggtagaga ttattcctca tgcgggtaca
     5401 tcggcgcaaa ccatcgccac tacggtaaaa ctggcgaaaa aacagggcaa aacgcccatt
     5461 gtcgtgcgtg acaaagccgg gttttacgtc aatcgcatct tagcgcctta cattaatgaa
     5521 gctatccgca tgctgaccga aggtgaacgg gtagagcaca ttgatgccgc gctagtgaaa
     5581 tttggttttc cggtaggccc aatccaactt ttggatgagg taggaatcga caccgggact
     5641 aaaattattc ctgtactgga agccgcttat ggagaacgtt ttagcgcgcc tgcaaatgtt
     5701 gtttcttcaa ttttgaacga cgatcgcaaa ggcagaaaaa atggtcgggg tttctatctt
     5761 tatggtcaga aagggcgtaa aagcaaaaaa caggtcgatc ctgccattta cccgttcatt
     5821 ggcgcacaag ggcaggggcg actctccgca ccgcaggttg ctgaacggtg tgtgatgttg
     5881 atgctgaatg aagcagtacg ttgtgttgat gagcaggtta tccgtagcgt gcgtgacggg
     5941 gatattggtg cggtatttgg cattggtttt ccgccatttc tcggtggacc gttccgctat
     6001 atcgattctc tcggcgcggg cgaagtggtt gcaataatgc aacgactggc cacgcagtat
     6061 ggttcccgtt ttaccccttg cgagcgtttg gtcgagatgg gcgcgcgtgg ggaaagtttt
     6121 tggaaaacaa ctgcaactga cctgcaataa gaaggtcaaa gctatatgaa tccgcgctga
     6181 atggcggagt gttggtcaaa atgtaaacgc atattgacta tacttacgcc attgaggtaa
     6241 aaaacagcgt ttcattcggt gaatggataa ggcacaatgc cggccaccgt tttctttctc
     6301 tggtttcaga tgaaagaaaa cgggcgaatc tggttaacaa aagcggtgca atatgcaagt
     6361 ttttatcatg cgtcacggcg acgcagccct cgatgccgcc agtgattcgg ttcgtcctct
     6421 gaccactaat ggttgtgacg aatctcgcct gatggcgaac tggctgaaag gtcaaaaagt
     6481 ggaaatcgaa cgcgttctgg tgagcccgtt cctgcgagcc gagcaaacac tggaagaagt
     6541 aggggattgt ctgaacctgc cttccagtgc agaagttctg ccggagttaa cgccctgcgg
     6601 cgatgttggt ctggtcagtg cctatttgca ggcgttgacc aatgaaggtg ttgcttcggt
     6661 actggtgatc tctcacttac cgttagtcgg gtatctggtt gccgagttat gtccgggcga
     6721 aacgccgcct atgtttacca cctcggccat tgccagcgta acgcttgatg aaagcggaaa
     6781 cggtacattc aactggcaga tgagcccgtg taatctgaag atggcaaaag ctatctgatg
     6841 tgttccaaat tatgcccaat gaggtagatt cattgggcat aattgctcat taggcagcgg
     6901 atatctgtca acctcactac tattgaagac tctccttatc tataaataaa acactttcta
     6961 cattcattcg gaaattattc ttctgcgcgt atcttgattt tgcccaaagt tacatgttga
     7021 aatgaataat gtctgtttga cactcattgt catttattaa tacagataat agagaaaata
     7081 gagttgaata atcaggcagg atgtaattga aacttgatta aattctggct atagtgttta
     7141 tatattatat tgtcaaaagt ggataaatgc caatcgcatt atatgttcat tgtttgtttt
     7201 ttaagctgtt gatattcatt attgatatac tttctatttt ttctaaatac ttattccgta
     7261 ttttttaagg atgtccttaa gataaccttt cttctgtcaa tatattaatt aaatctacat
     7321 ttactcttta gtaaaattaa attggctcag gggtgtggaa acatcactat gcggcccaca
     7381 ccaacgaata caattttcta cgacctgttc tttttgggtc gcatgccgta ttcaatggct
     7441 gggtataaac attggctatt gactgaatca atgtattgca atgtttatat ttttaattat
     7501 ttatagttta aggacaaata atgagtaagt ttgttaaaac agctattgct gtggcaatgg
     7561 tgatgggcgt gttcacctct acggcaacaa tcgctgcggg taacaacggt actgcacgtt
     7621 tttacggcac cattgaagac tcagtgtgtt ctatcgttcc ggacgatcac aaactggaag
     7681 ttgatatggg tgatatcggt gccgaaaaac tgaaaaataa cggcaccacc acgccgaaaa
     7741 gtttccagat tcgtctgcaa gattgcgtat ttgatactca ggaaacaatg accactacct
     7801 ttactggtac cgtttcttct gcaaatagcg gcaattatta caccattttc aataccgata
     7861 ccggtgcggc atttaacaat gttagcctgg cgatcggtga ctctctgggt acctcttaca
     7921 aaagcggcat gggtattgac cagaaaatag tgaaagacac ttctaccaac aaaggcaaag
     7981 cgaagcagac actgaacttt aaagcctggc tggtcggcgc agctgatgcg ccagatctgg
     8041 gtaattttga agcaaatacc accttccaga ttacttacct gtaaggcata cccccatagc
     8101 gacactgcta tgggggattt aaatggattt tgaccgaaca agaataaaaa cggatagaaa
     8161 cgtgtatgcc tgaccattct ctttttcgat tacggattct tccgtggtgc attgtgctgg
     8221 caatgtcagg gagttatagc agtgtctggg ctgaagacga cattcagttt gattcccgtt
     8281 ttctggaatt aaaaggcgac acaaaaattg atctgaagcg tttttccagc cagggatatg
     8341 ttgagcccgg aaaatacaat ttacaggttc aactaaataa acagccattg gcggaagagt
     8401 acgatattta ctggtatgct ggtgaagatg acgcgagcaa aagctatgct tgtctgacac
     8461 cggaactggt agcgcagttt ggtttaaaag aagacgtggc gaaaaatctg caatggagcc
     8521 acgatgctaa atgcctgaaa tccggtcaac tggaaggcat ggaaattaag gctgatttaa
     8581 gccagtccgc attagtcatt tcactgccac aggcttacct cgaatatact tatcccgact
     8641 gggatccgcc ttcacgttgg gatgacggca tctccgggat cgtcgcggac tacagcatca
     8701 acgcacaaac ccggcacgaa gaaaatggcg gtgatgatag taacgagatc agcggcaacg
     8761 ggacggtcgg ggttaacctg gggccgtggc gtatgcgtgc tgactggcag actaactatc
     8821 aacatactcg cagtaatgat gacgatgaat tcagcggcga tgaaactcaa aaaaaatggg
     8881 agtggagtcg ctactatgcc aggcgggcgt taccatcatt aaaagccaaa ctggcgctgg
     8941 gcgaggatta cctcagatcc gatatttttg atggttttaa ctatgttggt ggcagtgtca
     9001 gtactgacga tcaaatgttg cctcccaatc tgcgcggcta cgcgccagac atttccggcg
     9061 tggcacacac cacagcaaaa gtgaccgtca gccagatggg gcgtgtgatt tacgaaacgc
     9121 aggttccggc tggaccgttt cgtattcagg atcttggtga ttctgtctcc ggtacgttgc
     9181 atattcgcat tgaagaacag aacggccagg tgcaggaata tgacatcagc accgcctcga
     9241 tgccatacct cactcgtcca ggtcaggttc gctataagat catgatgggc cgtccgcaag
     9301 agtggggaca ccatgtcgag ggtgaatttt tttctggtgc tgaagcttcc tgggggatcg
     9361 ctaacggctg gtcgttatat ggcggcgcac tgggagatga aaactatcag tctgcggcgc
     9421 ttggcgtcgg tcgcgatttg tctacattcg gcgcggtcgc gtttgatgtt actcactcgc
     9481 acaccaaact ggataaagac accgcttatg gcaaaggttc gctggacggt aactccttcc
     9541 gtgtgagtta ttccaaagac tttgaccagc tcaacagccg cgttactttt gctggatatc
     9601 gcttctcgga agagaacttt atgaccatga gcgagtatct ggatgccagt gacagcggaa
     9661 tggtacgcac gggcaacgac aaagagatgt acaccgccac ttataaccag aacttccgcg
     9721 atgcgggtgt ttcggtttat ctcaactata cccgccatac ctactgggat cgcgaggagc
     9781 agacaaacta caacatcatg ctctcgcact atttcaatat gggcagtatt cgtaatgtca
     9841 gcatctcgat gactggctac cgttacgagt atgacaacca ggccgacaaa ggcatgtaca
     9901 tttcgctcag tatgccgtgg ggcgacaaca gtaccgttag ctataacggt actatggcag
     9961 tgggacggac agcagtcagg tcggttattt cagccgtgtc gatgacgcga ctcactatca
    10021 gttgaacgtc ggcaccagtg acaaacacac cagcgttgac ggctattaca gccatgatgg
    10081 ttcgctggcg caggttgacc tcagtgcgaa ctaccatgaa gggcaataca cctctgcggg
    10141 cttgtcgtta cagggcggcg caacgcttac tgcccacggt ggcgcactcc accgtaccca
    10201 gaatatgggc gggacacgct tgttgattga tgccgatggc gttgccgatg ttccggtaga
    10261 aggtaacggt gctgctgttt ataccaatat gtttggtaaa gccgtcgttt ctgacgtcaa
    10321 taactattac cgcaatcagg cgtatatcga cctcaacaaa ctgccggaaa acgccgaagc
    10381 aacccagtcg gtggtacaag ccacgctaac tgaaggggcg attggctacc gcaaatttgc
    10441 cgtcatcagt ggtcaaaaag cgatggcggt gctgcgttta caagacggca gccatcctcc
    10501 gtttggcgca gaagtaaaaa atgataacga gcagacagtg ggccttgtcg atgatgacgg
    10561 caatgtttat ctggctgggg tgaaacctgg cgaacacatg agtgtgttct ggagtggtgt
    10621 tgcgcattgc gatatcaacc tgccggaccc gctgcctgcc gatctgttta acggcttgtt
    10681 actgccatgc cagcataaag gcaacgtcgc gccggttatt cctgatgaca taaagcctgt
    10741 cattcaggag cagacgcaac aggtgacacc caccaatccc cctgtttctg tttcagctaa
    10801 ccaataacgt gattaaggaa tgatccatgt ctgatttact ttgctctgcc aaattgggtg
    10861 ctacgacgct agcgttactg cttagcgctg ccagtttgtc tgcccaggct tctgtaacgc
    10921 cagatcgtac ccgtttgata tttaatgaaa gcgacaaatc aatcagcgtg acgttgcgca
    10981 ataacgatcc gaaactgcct tatctggccc aaagctggat cgaagatgaa aagggtaaca
    11041 agatcagttc accgttaact gttctgccac cggtgcaacg catcgattca atgatgaacg
    11101 ggcaggtaaa agtgcaggga atgccggata tcaacaaatt gcctgccgat cgtgaaagcc
    11161 tgttctactt caacgtgcgt gagatcccgc caaaatcaaa caaagccaac acgctgcaaa
    11221 tagcactaca aacgcgtatt aagttgttct ggcgaccgaa ggcgctggag aacgtcagca
    11281 tgaaaaaccc ctggcaatat aaggttactt taacccgcaa cggtcaggaa tttaccgtca
    11341 ataacccgac gtcgtattac gtcattttca gtaatgccag cacacagaaa aatggcaatc
    11401 cggcagccgg tttctcgcca atagtgatgt cgccgaaaac ctctgcaccg ctgaatgtga
    11461 agatgggtag cgtgccggta ctgacctatg tcaacgacta tggcgctcgg atgccgctct
    11521 ttttcacttg caacggtaac a
//
LOCUS       DADLSB010000121        11142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.121, whole genome shotgun sequence.
ACCESSION   DADLSB010000121 DADLSB010000000
VERSION     DADLSB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.121"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(327..1991)
                     /gene="glnS"
                     /locus_tag="JN331_003056"
     CDS             complement(327..1991)
                     /gene="glnS"
                     /locus_tag="JN331_003056"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY7261140.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2194..4140)
                     /gene="nagE"
                     /locus_tag="JN331_003057"
     CDS             complement(2194..4140)
                     /gene="nagE"
                     /locus_tag="JN331_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY7261141.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAVVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAVGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISLVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4473..5273
                     /gene="nagB"
                     /locus_tag="JN331_003058"
     CDS             4473..5273
                     /gene="nagB"
                     /locus_tag="JN331_003058"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7261142.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5333..6481
                     /gene="nagA"
                     /locus_tag="JN331_003059"
     CDS             5333..6481
                     /gene="nagA"
                     /locus_tag="JN331_003059"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7261143.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6490..7710
                     /gene="nagC"
                     /locus_tag="JN331_003060"
                     /gene_synonym="nagR"
     CDS             6490..7710
                     /gene="nagC"
                     /locus_tag="JN331_003060"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY7261144.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLTSEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7758..8510
                     /gene="nagD"
                     /locus_tag="JN331_003061"
     CDS             7758..8510
                     /gene="nagD"
                     /locus_tag="JN331_003061"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAY7261145.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEEAVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8907..10571
                     /gene="asnB"
                     /locus_tag="JN331_003062"
     CDS             8907..10571
                     /gene="asnB"
                     /locus_tag="JN331_003062"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY7261146.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10951..11027
                     /locus_tag="JN331_003063"
     tRNA            10951..11027
                     /locus_tag="JN331_003063"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10985..10987,aa:Met,seq:cat)
     gene            11036..11120
                     /locus_tag="JN331_003064"
     tRNA            11036..11120
                     /locus_tag="JN331_003064"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11070..11072,aa:Leu,seq:tag)
BASE COUNT         2784 a         2864 c         2810 g         2684 t
ORIGIN      
        1 cagcaagatg aaagcggtta cattttattt aattatgttt tgtgatttta aatttatttt
       61 atggataaat tgcgaagcgg aattaattcg cgaagaaaag cagaaaaaac gccgcagagc
      121 gcggcgtttg ggattgtttg ctatttcttg ccggatgcgg cgtgaacgcc ttatccggcc
      181 tacaaaagca cgcaaattca atatattgca gagatcatgt aggcctgata agcgaagcgc
      241 atcaggcaat ttagcgtttg aaatttattg tctatttatt gccggatgcg acgtaaatgc
      301 ctcacccggc ttggtagaac ttaaaattac tcgcctactt tcgcccaggt atcacgcagc
      361 ccaacggtgc ggttaaatac cggtttttcg gccgtggaat ggcggctgtc gaggcagaag
      421 taaccttcac gctcaaactg gaatgcttta cccgctaccg catctttcag cgacggttca
      481 gcaaagccct gtttgatcac cagcgattcc gggttaatca ccgacaggaa atcatccgca
      541 gcacctgggt taggcacgct gaacagacga tcatacaaac ggatttcaac cggcagcgca
      601 tgtgccgcgc tcacccagtg aataacacct ttgactttac gaccatctgc cggatctttg
      661 cttaaggtgt cggcgtcata agtacagaag atggtggtga tattaccttc ggcatctttc
      721 tccacgcgtt cagccttaat gacgtaagca ttacgcagac gcacttcttt acccagcacc
      781 agacgtttgt actgcttgtt agcttcttcg cggaaatcgg cgcgatcgat ccaaatttca
      841 ccgctaaacg gcacctgacg gctccccatt tccggtttgt tcggatggtt cggcatggtg
      901 accatttcgc cttcgccctg atagttttcg ataaccagtt tcaccggatc gataaccgcc
      961 attgcgcgcg gcgcattttc gttgagatct tcacggatgc aggattccag cgacgccatc
     1021 tcaatggtgt tgtcctgctt ggtcacgccg atgcgtttgc agaactcacg aatagaagcc
     1081 gcagtgtaac cacgacgacg cagaccggaa atggtcggca tacgcgggtc atcccagcct
     1141 tcaacgtgct tgtcggtcac cagcaggttc agcttacgct tggacatcac agtgtattcc
     1201 agattcaggc gcgagaattc gtactggcgc ggatgaacag gaatggtgat gttatccagt
     1261 acccagtcgt acagacgacg gttgtcctgg aactcaagcg tacacagaga gtgcgtaata
     1321 ccttccagcg catcgctgat gcaatgggtg aagtcgtaca tcgggtagat gcaccacttg
     1381 ttgccagtct ggtggtgttc agcaaattta atgcggtaca gcaccggatc gcgcattacg
     1441 ataaatggcg acgccatgtc gattttcgca cgcaggcagg ctttaccttc ttcaaaacca
     1501 ccggcacgca ttttttcgaa cagcgccaag ttctcttcaa cgctgcggtc gcggtacggg
     1561 ctgtttttac ccggctgcgt cagggtaccg cggtattcgc ggatctgttc cggtgtcagt
     1621 tcatcaacgt acgccaggcc tttattgatc agttcgatcg cataggcgtg gagctgatca
     1681 aaataatcgg aggagtaacg gacgttacca gaccagtgaa aacctaacca ctctacgtcg
     1741 tttttgatcg aatcgacata ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc
     1801 aggttgcact ggcctttata gtcctgggcg atcccgaagt tcaggcagat agatttcgca
     1861 tggccaatat gcagatagcc attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc
     1921 ttaccactgg ccagatcttc atcgatgatc tgacggataa agttagtcgg gcgggcttct
     1981 gcctcactca tcgtggattc ctcaaagcgt aaacaacgta taacggcgta tgatcttata
     2041 agcgggacag gctgacaact gttagttttt taaaattgat aatctaacaa ggattatggg
     2101 gatgattgat gaaaaaaaaa gcggcagaga agatctccac cgcttgtcat tgttggatgc
     2161 gacgctgccg tgtcgcatca ggcataaagc agattacttt ttgatttcat acagcggtgt
     2221 ttgacccgcc acaacatggc cctgagcttt aatgatcaag ccactgaaat cgtcgatatt
     2281 gctgcaaacc accaggctaa tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat
     2341 ttccagaatc ggttgccctg cgcttacctg cgcaccctct tccaccagac gtttaaagcc
     2401 tttaccttcc agcgctacgg tgtcgatacc catatggacg acgatctccg cgcctttttc
     2461 ggtttccagg cagaacgcgt ggttggtgtt gaagattttc acgattgtcc ctgcggctgg
     2521 tgatacgacg attttatctg tcggtttcac cgccacaccg tcacccaccg ctttgctggc
     2581 gaatgcttcg tcaggaacct gatccagtgc cacgacatca ccggtaatcg gcgataccag
     2641 ctccgcgata gataccgcgt ttggtacagc ctgcggtttt gctacaggcg cggcagttgc
     2701 cggagttgct tcagctgacg cagcggctac cggaccacgg gcaacgactt tcttcatcgc
     2761 atcgccgatg gattctgctt tcgcgccaac aatcacctga atagtctgtt tgttcagttt
     2821 cactacacca gaagcaccca gacgtttaca catcgtatcg ttgacgcggg cagagtcagc
     2881 cacggtcagg cgcagacggg taatacaggc atcaatcgct ttcaggttgt cagtaccgcc
     2941 aaccgcagca atatagttgg ttgccagttg agtcagacct tcttcagtat tgctgttggc
     3001 ttcttcagta acgatctcgt cttctttatc ttcacgaccc ggcgttttca ggttgaacat
     3061 gcggataacc aaactgaaca ccacgaagta gatagcgaag aagataacgc ccatcaccag
     3121 cagcatccag acgttctggc tggcggccgg caggttatac atcaacgcgt agtcgatagc
     3181 ccccgcagag aaagagaagc cggcatggat acccagcagc gttgccacaa acaggctgat
     3241 accggtcagc agtgcgtgca ggaggtacag cagcggcgca aggaacatga acaggaattc
     3301 cagcggctca gtcacaccgg tcaggaacgc agtaacagca acagaaagca gcatcccgcc
     3361 aaccatcgga cgacgctctt tcggtgctgc gaagtacatc gccagcgccg cacccggcag
     3421 accgaacatc atgatcggga agaagccgga catgaacatc cccgcggtgc cgtcaccagc
     3481 gtagaaacgg ttgatgtcgc cgtggaaaac cgtacccacc gcgttggtga attcaccaat
     3541 ctggaaccag gcgatggtgt tcagcacctg atgcagaccg gttgggatca gcagacggtt
     3601 gatgaaacca aagataccgg aacccagcgc gcccgcagaa acgatccact cgccgcctgc
     3661 atggatagcg tgctgtaccg gcggccagac gtaaccaaaa atggccgcca gcaccagaca
     3721 gaagaagccg gtggcgatcg gcacaaagcg tttgccgccg aagaagctca ggaagtccgg
     3781 cagtttaata tcggaccaac ggttataggc tgcaccacca accagaccgg taatgatacc
     3841 cgccagtaca cccatgttaa tttctgggtt gatggtcacc accgctttgg ttaacacaaa
     3901 gtaacctacc gcacccgcca gcgccgccgc acctgcgctg tctttcgacc agctggatgc
     3961 cacaccgatg gcgaagatta atgcgaggtt atcaaaaatt gcaccgcccg cctgggcaat
     4021 aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc aacagcgccg ccaccggcag
     4081 caccgcgata gggagctgta acgccctacc gagtcgctgg aaaaaaccta aaatattcat
     4141 cttattcccc ctacgagaac cctatttggc tcgtttaaag ccgtattttt attttgctgc
     4201 aaattttact gccgatgttc tgtaatcaga ttgttagatc atctgctaca gagtgtgtga
     4261 aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt gagttttgtc accaaatatc
     4321 gttatcatca ctccctttta ctggctaaac cagaaaactt attttatcat tcaaaaaatc
     4381 aagtcggatt gacgcctgtc tgcgcaaatc caggttacgc ttaaagatgc ctaatccgcc
     4441 aacggcttac attttactta ttgaggtgaa taatgagact gatccccctg actaccgctg
     4501 aacaggtcgg caaatgggct gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccaa
     4561 ctgccgatcg tccgtttgta ctgggcctgc cgactggcgg cacgccgatg accacctata
     4621 aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt taagcacgtt gtcaccttca
     4681 acatggacga atatgtcggt ctgccgaaag agcatccgga aagctactac agctttatgc
     4741 accgtaattt cttcgatcac gttgatattc cagcagaaaa catcaacctt ctcaacggca
     4801 atgccccgga tatcgatgcc gagtgccgcc agtatgaaga aaaaatccgt tcttacggaa
     4861 aaattcatct gtttatgggc ggtgtaggta acgacggtca tattgcattt aacgaaccgg
     4921 cgtcttctct ggcttctcgt actcgtatca aaaccctgac tcatgacact cgcgtcgcga
     4981 actctcgttt ctttgataac gatgtcaatc aggtgccaaa atatgccctg actgtcggtg
     5041 ttggtacact gctggatgcc gaagaagtga tgattctggt gctgggtagc cagaaagcac
     5101 tggcgctgca ggccgccgtt gaaggttgcg tgaaccatat gtggaccatc agctgtctgc
     5161 aactgcatcc gaaagcgatc atggtgtgcg atgaaccttc caccatggag ctgaaagtta
     5221 agactttaag atatttcaat gaattagaag cagaaaatat caaaggtctg taattgttat
     5281 ccctgccctg gttccttgct cagggcaata ttttttaaaa tcgggggtca gaatgtatgc
     5341 attaacccag ggccggatct ttaccggcca cgaatttctt gatgaccacg cggttgttat
     5401 cgctgatggc ctgattaaaa gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca
     5461 acgttctctg aacggggcca ttctctcccc cggttttatc gatgtgcagt taaacggctg
     5521 cggcggcgta cagtttaacg acaccgctga agcggtcagc gtggaaacgc tggaaatcat
     5581 gcagaaagcc aatgagaaat caggctgtac taactatctg ccgacgctta tcaccaccag
     5641 cgatgagctg atgaaacagg gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa
     5701 tcaggcgtta ggtctgcatc tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca
     5761 taatccgaat tttgtgcgta agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc
     5821 cgacgtcatt accaaagtga ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa
     5881 actggcaaat gccgggattg tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc
     5941 aaaagccggt ttccgcgcgg ggattacctt tgccacccat ctgtacaacg cgatgccgta
     6001 tattaccggt cgtgaaccgg gcctggcggg cgcgatcctc gacgaagctg acatttattg
     6061 cggtattatc gctgatggcc tgcatgttga ttacgccaac attcgtaacg ctaaacgcct
     6121 taaaggcgac aaactgtgtc tggttaccga cgccaccgcg ccagcaggtg ccaacattga
     6181 acagttcatt tttgcgggta aaacaatata ctaccgtaac ggactttgtg tggatgagaa
     6241 cggtacgtta agcggttcat ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca
     6301 ttgcggtatc gcactggatg aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat
     6361 tgacgttgaa aaacgtctcg gcacgctcgc cgcaggtaaa gtagccaacc tgaccgcatt
     6421 cacacctgat tttaaaatca ccaagaccat cgttaacggt aacgaggtcg taactcaata
     6481 agagaaagta tgacaccagg cggacaagct cagataggta atgttgatct cgtaaaacag
     6541 cttaacagcg cggcggttta tcgcctgatt gaccagtacg ggccaatctc gcggattcag
     6601 attgccgagc aaagccagct tgcccccgcc agcgtaacca aaattacgcg tcagcttatc
     6661 gaacgcgggc tgatcaaaga agttgatcag caggcctcca ccgggggccg ccgcgctatc
     6721 tccatcgtca ccgaaacccg caatttccac gcaatcggcg tacggcttgg tcgtcatgac
     6781 gccaccatca ctctgtttga tctcagcagc aaagtgctgg cagaagaaca ttacccgctg
     6841 ccggaacgta cccagcagac gctggaacat gccctgctga atgccattgc tcagtttatt
     6901 gatagctacc agcgcaagct gcgcgagcta atcgccattt cggtgatcct gccagggctt
     6961 gttgacccgg acagcggcaa aattcattac atgccgcata ttcaggtaga aaactggggg
     7021 ctggtagaag cactggaaga acgttttaaa gtgacctgtt tcgttggtca cgatatccgt
     7081 agtctggcgc tggcggagca ctacttcggt gcaagtcagg attgcgaaga ctccattctg
     7141 gtgcgtgtcc atcgcggaac cggggccggg attatctcta acgggcgcat ttttattggc
     7201 cgcaacggta acgtcggtga aattggccat attcaggtcg aaccgctggg tgaacgctgc
     7261 cactgcggca actttggctg cctggaaact atcgccgcca acgctgccat tgaacaacgg
     7321 gtgttgaatc tgttaaagca gggctaccag agccgtgtgc cactggacga ctgcaccatc
     7381 aaaactatct gcaaagccgc gaacaaaggc gatagcctga cctcggaagt gattgagtat
     7441 gtcggtcgtc atctgggtaa aaccatcgcc attgctatca acttatttaa tccgcaaaaa
     7501 attgttattg ctggtgaaat caccgaagcc gataaagtgc tgctccctgc tattgaaagc
     7561 tgcattaata cccaagcgct gaaggcgttt cgtactaatc tgccggtggt acgttctgag
     7621 ctggatcacc gctcggcaat cggcgctttt gcgctggtaa aacgcgccat gctcaacggt
     7681 attttgctcc agcatttgct ggaaaattaa tgtgctttta tagtggcgct tattgttgtc
     7741 aatattctgg gtagtccatg accattaaaa atgtaatttg cgatatcgac ggcgtgctga
     7801 tgcacgataa cgtcgccgta ccgggtgcag cggaattttt gcacgggatt atggataaag
     7861 gcctgccgct ggtgttgctg accaactatc cttcgcagac tgggcaagat ctggcgaacc
     7921 gctttgccac tgccggtgtc gatgtgccgg atagcgtgtt ttatacctct gcgatggcga
     7981 ccgccgattt cctgcgtcgc caggaaggca agaaagcgta tgtggtgggc gaaggcgcac
     8041 tgattcatga actgtacaaa gccggtttca ctattaccga tgtgaaccct gatttcgtga
     8101 ttgttggcga aacacgttcc tacaactggg acatgatgca taaagcagcc tatttcgtcg
     8161 ctaacggtgc acgctttatc gccaccaacc cggacaccca cgggcgcggt ttttatcccg
     8221 cttgtggcgc gttgtgtgca gggattgaga aaatctccgg gcgcaaaccg ttctatgttg
     8281 gtaagcccag cccgtggatc atccgcgcgg cattaaacaa aatgcaggcg cattcggaag
     8341 aagcagtgat tgtcggcgat aacctgcgta ccgatattct ggcgggcttc caggccggtc
     8401 tggagacgat tctggtgctt tctggtgttt catcgctcga cgatatcgac agtatgccgt
     8461 tccgcccaag ctggatttac ccgtcagtcg ctgaaatcga cgttatctga ataccaccca
     8521 cgtctgcgga cgtggttggc tctgatgaca aacgccaaac tgcctgatgc gctacgctta
     8581 tcaggcctac gcagctcctg caatatattg aattttcatg cttttgtagg ccggataagg
     8641 cgttcacgcc gcatccggca tgaacaaatc gcactttatc agcaatttgg cccccgtaaa
     8701 atataaccat tcaataaaac ccgctaaaaa ttatcgattc atcaataaat acatcctcac
     8761 accagaccct ttttcaccaa tcgacaatca ccattacgtt ttttattttt gcactgccaa
     8821 atagcttgcg cacagtactg ctttgcggca tttttttaaa caagcaaaca caacaagcaa
     8881 caaataccag gttaacggag aaggttatgt gttcaatttt tggcgtattc gatatcaaaa
     8941 cagacgcagt tgagctgcgt aagaaagcac tcgagctgtc acgcctgatg cgtcatcgtg
     9001 gcccggactg gtccggtatt tatgccagcg ataacgccat tctcgctcac gaacgtctgt
     9061 caattgttga cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa acccacgtac
     9121 tggcggtaaa cggtgaaatc tacaaccacc aggcattgcg cgctgaatat ggcgatcgtt
     9181 atcagttcca gacagggtct gactgtgaag tgatcctcgc gctgtatcag gaaaaagggc
     9241 cggaatttct cgacgacttg cagggcatgt ttgcctttgc cctgtacgac agcgaaaaag
     9301 atgcctacct gattggtcgc gaccatctgg ggatcatccc actgtatatg gggtatgacg
     9361 aacacggtca gctgtatgtg gcctcagaaa tgaaatcgct ggtgccagtt tgccgcacga
     9421 ttaaagagtt cccggcgggg agctatttgt ggagtcagga cggcgaaatc cgttcttatt
     9481 atcatcgcga ctggttcgac tttgatgcag tgaaagataa cgtgaccgac aaaaacgagc
     9541 tgcgtcaggc actggaagat tccgttaaaa gccatctgat gtctgatgtg ccttacggtg
     9601 tgctactttc tggtggtctg gattcctcaa ttatttccgc tatcaccaag aaatacgcag
     9661 cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg gccgcagtta cactcctttg
     9721 ctgtaggtct gccgggttca ccggatctta aagcagccca ggaagtggca aaccatctgg
     9781 gcacggtgca tcacgaaatt cacttcactg tacaggaagg tctggatgcc atccgcgacg
     9841 tgatttacca catcgaaact tatgatgtga ccactattcg cgcttcaaca ccgatgtatt
     9901 taatgtcgcg taagatcaag gcgatgggca ttaaaatggt gctgtccggt gaaggttctg
     9961 atgaagtgtt tggcggttat ctttacttcc ataaagcgcc caacgctaaa gaactgcatg
    10021 aagagacggt gcgtaaactg ctggccctgc atatgtatga ctgcgcgcgc gccaacaaag
    10081 cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct cgacaaaaaa ttccttgatg
    10141 tggcgatgcg cattaacccg caggataaaa tgtgcggtaa cggcaaaatg gaaaaacaca
    10201 tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt ggcctggcgg cagaaagagc
    10261 agttctccga tggcgtcggt tacagttgga tcgacaccct gaaagaagtg gcggcgcagc
    10321 aggtttctga tcagcaactg gaaactgccc gcttccgctt cccgtacaac acgccgacct
    10381 caaaagaagc gtatctgtac cgcgagatct ttgaagaact gttcccactt ccgagtgccg
    10441 ctgagtgcgt gccgggcggt ccttccgtcg cgtgttcttc agctaaagcg atcgaatggg
    10501 atgaagcgtt caaaaaaatg gacgatccgt ctggtcgtgc ggttggcgtt caccagtcgg
    10561 cgtataagta agaaatgcgg tactgccccg gagaaatccg gggctgtttt agttattgcg
    10621 ctgcttgttg attctgatat gcagtaagga aatgttccgg gtttgccaca tcactgtttg
    10681 acaactcatc actgtaggcc agtatttcat agattaattt cccaccacta ttgttgaatt
    10741 ccgaatttca tcctgtacga caaaagaatg gctgtagggt ggggaaaaaa cacaaagtaa
    10801 acaataattg acgaatatag cgccacgctg ttcgcaacct aaccaaacag tcactttcga
    10861 gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct atacgcataa tgcgccccgc
    10921 aacgccgata aggtatcgcg aaaaaaagat ggctacgtag ctcagttggt tagagcacat
    10981 cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccatct tttttgcggg
    11041 agtggcgaaa ttggtagacg caccagattt aggttctggc gccgcaaggt gtgcgagttc
    11101 aagtctcgcc tcccgcacca ttcaccagaa agcgttgtac gg
//
LOCUS       DADLSB010000122        10888 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.122, whole genome shotgun sequence.
ACCESSION   DADLSB010000122 DADLSB010000000
VERSION     DADLSB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.122"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..186
                     /locus_tag="JN331_003065"
     CDS             <1..186
                     /locus_tag="JN331_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7261147.1"
                     /translation="QLRPRNPETVRRWVRECRIFPPPVKDGREYLFHESAVKVDLNRP
                     VTGSLLKRIRNGKKAKS"
     gene            164..1237
                     /locus_tag="JN331_003066"
     CDS             164..1237
                     /locus_tag="JN331_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7261148.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     VSEAIQANIELLSENRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLNDISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYIEIYHAAEPLPIWLRLAMDLAVITGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKIAIPLTLTIDALNISLVDTLQKCREASGSETVIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDKIEIDK"
     gene            complement(1295..1855)
                     /locus_tag="JN331_003067"
     CDS             complement(1295..1855)
                     /locus_tag="JN331_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY7261149.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLHV"
     gene            complement(1852..3540)
                     /locus_tag="JN331_003068"
     CDS             complement(1852..3540)
                     /locus_tag="JN331_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY7261150.1"
                     /translation="MSGEKKTKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(3689..6316)
                     /gene="gyrA"
                     /locus_tag="JN331_003069"
     CDS             complement(3689..6316)
                     /gene="gyrA"
                     /locus_tag="JN331_003069"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY7261151.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYYTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6463..7185
                     /gene="ubiG"
                     /locus_tag="JN331_003070"
     CDS             6463..7185
                     /gene="ubiG"
                     /locus_tag="JN331_003070"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HAY7261152.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPSGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(7313..10888)
                     /gene="yfaL"
                     /locus_tag="JN331_003071"
                     /pseudo
     CDS             complement(7313..10888)
                     /gene="yfaL"
                     /locus_tag="JN331_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2614 a         2970 c         2663 g         2641 t
ORIGIN      
        1 cagttacgtc cacggaaccc ggaaacggtt cgccgctggg tacgcgagtg caggatattc
       61 cctcctccgg ttaaggatgg aagagagtat ctgttccacg aatcagcggt aaaggttgac
      121 ttaaatcgac cagtaacagg tagccttttg aagaggatca gaaatgggaa gaaggcgaag
      181 tcatgagcgc cgggatttac cccctaacct ttatataaga aacaatggat attactgcta
      241 cagggaccca aggacgggta aagagttcgg attaggcaga gacagaagga tagcagtttc
      301 agaggctatt caggccaata ttgagttgct atctgagaac aggcgtgagt cactgataga
      361 caggattaaa ggcgctgacg caatcactct tcatgcgtgg cttgaccgat atgaaacaat
      421 cctcagcgag aggggtatca gaccgaaaac tctactcgac tacgccagca aaatcagggc
      481 gattcgaaga aaattgccgg acaaaccgct caatgacata tcaacgaaag aggtggcagc
      541 aatgctaaac acctacgtag cagaaggtaa agcggcttcc gcaaaattaa tcaggtcaac
      601 ccttgttgac gtttttcgtg aagcaatagc cgaggggcat gtggctacga atccggtaac
      661 agcaacccgc acagcaaagt cagaagtaag gcgctcaagg cttacagcta atgagtatat
      721 cgagatttac catgcagccg aacctctccc tatctggctg aggctggcaa tggatttggc
      781 cgtcattaca gggcagagag tgggcgattt atgcagaatg aagtggtcag acataaacga
      841 caaccatctt cacattgagc agggtaaaac aggagccaag attgccattc cgctgacgct
      901 aacgattgac gcgctcaaca tctcactggt tgatacacta cagaaatgca gggaggccag
      961 cggcagtgaa actgtaatcg cctcaacgca tcacgaaccg ctttccccga aaacagtatc
     1021 gaagtatttt acaaaggcga gaaatgcctc cggactctca tttgatggag acccgccaac
     1081 attccatgaa ctgcgtagcc tgtcagcgag gctataccgg aaccagattg gcgataaatt
     1141 tgctcaacgt cttctcggac ataaatcaga ttcaatggcg gcgcggtata gggacagccg
     1201 tggacgggaa tgggacaaaa ttgaaatcga caaatgattt tattttgacc aataatgacc
     1261 tgctcatctt aacagattga taattaatgc aattttaaac atgcaacttc catgtcataa
     1321 gttgttgcag actgactgcg cgcattccgt tgtcagtttt cgtggcgctt ccggtgtggt
     1381 agatgacgta acgccccatc cagaccatta agtgctgggc atcgccctga tcgaaaaaaa
     1441 tcatatcgcc aggcagcgcc tggtttatgt cttggccaat aaactgactg ttgtactgaa
     1501 tcaaattaat cgcggtcacg taggggccgt ttttcccgtt cccctgattc cagttttgcg
     1561 ccagttgacg ctgttcaggt gttagcgtca tctctggcgg caaatactgg ctagataaac
     1621 cgttactttt tagccattta ctgtcgtgaa ctttcagcgt ctcgttcgcc gcaaatcgca
     1681 ccaagcccgc acaatcctgc tgataccagc gtggacttgg cccctggcgg agctgttctt
     1741 gtgcaatgcg tacaaaccag gcgcgaaaca gcccggattg ttcgacattc agcatttcgc
     1801 tatgggcaac aacacaacac agccagcaaa tcagcgccag cagcccgtgt ctcatagcgg
     1861 ctgccaggtt atgggtagcc actgccaggc gacaccgggt tccatctggg ccagcttcat
     1921 gacataacgc ggttgttgag ataaagcgtc cagcttcggc attaataaag tttgtgcggc
     1981 gttataaaaa accggttcga gattcttcgg cagactggtc aacgtttcgt tacgcagcag
     2041 tttcgccacg ccttgtggat tgatatagag cggaacgatg ccatcagtgg gtattacatc
     2101 caccattgcc gggcgggttt tattcagtgt ttgcagggcg ttattaacca gcgtgtcatc
     2161 gagggagaaa agcagcgttt tgttttgcat cgccagcgac acacggaaaa aataatccga
     2221 cattaattga tcgggttgcg ccgcctgcgc tttcggatac tgaccgtatc gggaactcac
     2281 ttcgcgacac cagatttgcg cttcgccctg ctgagtctgg cttaccggca aaacaccttc
     2341 tggcgctttg ctttcgtgcg caccaatatt ttgcgtaaac agtttccctg gcaattgcgc
     2401 ctgttcggca gtgccatcaa actgaccgac aaacagcggg gtttgcaatt ttgagtcttc
     2461 ataccagcac agccccgcgg caccgtctaa cgccccattc agcttgtcgt tttcctggct
     2521 gatgtgcgaa agcatctctt cggcaatacc gtgtgaatac ggcaccgcca cacagaagct
     2581 ggctccggca ggcatactgt tccagaccgg cgtaaaatcg aaactggcat ctacgctggc
     2641 ggattcatca tttaacgcca caaaactgtg ccagccgtcg ttacccattt cgaagcgtac
     2701 gccagcaaaa gaaggcatta accgctggta gccaaaaccc agcaacctgg cgctggccac
     2761 gatgcgcagg cgtactggcg ttttttcggc agtacgctct tccaggccaa agctggcttg
     2821 ccagcgtttt ttaccgctca acaaatcact tgcgatcgcc gtggcttcgg tatcctgctg
     2881 atcatcttta aacaacatat ccgtactgga aaacaccagc atcttgtcct gatatgtcgc
     2941 gaacatcagg gcgttattgc cgttatagcg caactgataa acaggaatag tttcactatt
     3001 tatcttgatg ctactgattt ccgttttgct taactggctg tcgctggtag cggcaaacaa
     3061 caatggctcc agcagtttgc ttaaaccact gcgctggatc agcaccatgt aatgtgaaag
     3121 atggccttgt ttatcgtgcc atagcgccgc ctgcgcgggc tgatctaaga gtgacgaaaa
     3181 gagcttatct ttcagcgtga gatcgtgttc atagacaatg cggcgaatgc tgccttcaat
     3241 gcccagacga tcggcatgat tctgataata gaaaacgaaa tcttcgctca gaacatcgtg
     3301 gagaaacgga atggtgagga gatctttggg aagctggctc agagagtcgc tgtctagaaa
     3361 gaggtccggc tcattgagat cgatttgcag attgttgtgc accaccagcg gcgacaacgt
     3421 tttttctggc ccactgccag catattgcaa cgcccagacg ccagcggaaa gcagtgctat
     3481 tgcgccaaaa cctacaagac catagaaccg ccagcctttc gtcttttttt caccactcat
     3541 tgccacattc cttgtgtata gccagccatt ttttacgggc acagccaaac tttaccgcgc
     3601 cctaatacga caaaagccca gactttgcag cctggacttt tcaattcaaa caagggagat
     3661 agctcccttt tggcatgaag aagtaaaatt attcttcttc tggctcgtcg tcaacgtcca
     3721 cttccggagc gatttcatca tccccttccg cggcactgcc gtcgatggta tccagatctt
     3781 cctcgtcaac cggttcagca acacgctgca gacccactac gttttcatct tccgcagtac
     3841 ggatgaggat cacgccctgg gtgttacggc ccacgatgct gatttctgaa acgcgagtac
     3901 gtaccagcgt accggcatcg gtgatcatca tgatctggtc gcagtcatct acctgcaccg
     3961 cgccaaccac taaaccgtta cgttcggtaa ccttgatgga gataacccct ttcgtcgcac
     4021 gcgacttggt tgggtattcc gccactgcag tacgtttacc gtaaccgttt tgcgttgcgg
     4081 tgaggattgc gccatcgcca cgaggcacga tcagagagac gactttatcg ccttcaccta
     4141 agcgaatacc gcgcacacca gtggtattgc agcccatcgc acggacagaa gactctttaa
     4201 agcgcaccac tttaccttcg gcggagaaca gcattacttc atcttcgcca ctggtcagat
     4261 caacgccgat cagctcatcg ccgtcaacca gtttgatcgc cactttaccg gcggtacgca
     4321 gacggttgaa ttcggtgagg acggttttct tcacagtacc gttagcggtc gccatgaaga
     4381 ctttcacgcc ttcttcaaac tcggtcaccg gcaggatcgc ggtgatacgt tcgtcctgtt
     4441 ccagcggtag caggttgacg atcggacgac cgcgcgcgcc acgagtggct tccggcaact
     4501 ggtaaacttt catcgaatag acgcgaccac ggctggagaa gcacagaata tggtcgtgcg
     4561 tgttcgccac cagcagtcgg tcgataaagt cttcttcttt aatacgtgcg gcagatttac
     4621 ctttcccgcc acgacgctgc gcttcgtatt cagaaagcgg ctgatattta acgtagccct
     4681 ggtgagagag cgtcacaacc acatcttcct gggtgatcag atcttccagg ttgatgtctg
     4741 cgctgttggc ggtgatttca gtacgacgtt tgtcaccgaa ctgttcacga accagctcca
     4801 gctcttcgcg gatcacttcc atcagacgat cggcgctacc aagaatacgc aacagttccg
     4861 cgatctgatc cagcagctct ttgtattcgt cgagcagttt ttcgtgctca aggccggtca
     4921 gtttctgcaa acgcagatcc agaatcgcct gagcttgctg ttcggtcagg tagtacagac
     4981 catcacgcac gccgaactct ggctccagcc attccggacg cgcagcatcg tcgccagcac
     5041 gttccaacat ggcggcaacg ttgcccagct gccacggatt agcaaccagc gcagttttcg
     5101 cttctgcagg cgtcggcgca tgacggatca gttcgatgat cgggtcaatg ttcgccagcg
     5161 ccacggctaa tgcttcaagg atatgagcac gatcgcgagc tttacgcagt tcgaaaatag
     5221 tacgacgggt caccacttca cggcggtgac gaacaaacgc cgcgatgatg tctttcaggt
     5281 tcatgatctt cggctgacca tggtgcaatg ccaccatgtt gataccgaaa gaaacctgca
     5341 actgggtctg ggagtagagg ttgttgagca caacttcacc gaccgcatcg cgtttcactt
     5401 caatcacgat gcgcataccg tctttgtcag actcgtcacg cagcgcgctg atgccttcca
     5461 cgcgtttttc ttttaccagt tccgcaatct tctcgatcag gcgcgctttg tttacctgat
     5521 acggaatttc gtggacgata atggtttcac gtccggtttt ggcgtcaact tccacttcag
     5581 cacgggcgcg gatatatacc ttgccgcgac cggtacggta agcttcttca ataccgcgac
     5641 gaccgttaat gattgccgcc gtcgggaagt ccggccccgg gatgtgttcc atcagccctt
     5701 caatgctgat gtcttcatca tcgatatacg ccagacaacc gttgatgact tccgtcaggt
     5761 tgtgcggcgg gatgttggtt gccataccta cggcgatacc ggaagaaccg ttcaccagca
     5821 ggttaggaat tttggttggc atgacgtcgg gaattttttc cgtgccgtca tagttatcaa
     5881 cgaaatcgac cgtctctttt tcgagatcgg ccatcagttc atgggcaatt ttcgccagac
     5941 ggatttccgt ataacgcatt gccgccgcag agtcgccgtc gatggaaccg aagttaccct
     6001 gaccgtctac cagcatgtaa cgcagcgaga atggctgcgc catacggacg atcgtgtaat
     6061 aaaccgccga gtcaccatgg ggatggtatt taccgattac gtcaccaacg acacgggcag
     6121 attttttata ggctttgttc cagtcattgc ctagtacgtt catggcgtaa agtacgcgac
     6181 ggtgtaccgg cttcaggcca tctcggacat ctggcagcgc acggccaaca atgaccgaca
     6241 tcgcataatc cagataggag ctctttagct cttcctcaat gttgaccggt gtaatttctc
     6301 tcgcaaggtc gctcatctaa ccgctatccc tctactgtat cccggattca aaggtcgcaa
     6361 attataacac agccgcgcag tttgaggtaa acttatacgc tttattcaca tccaatgcct
     6421 gatatactcg tttgtcttgc caattacgga gtagaagtgc caatgaatgc cgaaaaatcg
     6481 ccggaaaacc ataacgtaga ccacgaagag atcgctaaat ttgaagccgt cgcctcccgc
     6541 tggtgggatc ttgaaggtga gttcaaaccg ttgcaccgca ttaacccgct gcgtctgggc
     6601 tatattgccg agcgtgctgg cggtttattt ggcaaaaagg tgctcgatgt cggttgtggc
     6661 ggcggcattc tggccgagag tatggcgcgc gaaggcgcga cggtgaccgg tctggatatg
     6721 ggctttgagc cattgcaggt ggcaaagctg cacgcgctgg aaagcggcat tcaggtggat
     6781 tacgtgcagg aaaccgtgga agagcacgcg gcaaaacatg ccgggcagta tgatgtggtg
     6841 acctgcatgg agatgctgga gcacgtaccc gatccgcagt cagtggtcag agcctgtgcg
     6901 caactggtga aaccaagcgg cgatgtcttt ttctcgacgc ttaaccgcaa cggcaagtca
     6961 tggctgatgg cggtggttgg cgcggaatat attttgcgca tggtgcccaa aggtacgcat
     7021 gatgtgaaga agtttattaa accggcagag ctgcttggct gggtggatca gaccagttta
     7081 aaagagcgac atataaccgg gctacattac aacccgatca ctaattcctt taagctcggc
     7141 cccggcgtgg atgtgaacta tatgctgcac acgcagaata agtgaggttg atgtttggcc
     7201 gcgccaatgc ctgatgcgac gcttgcagcg tcttatcagg cctacaaatg ctctccgtag
     7261 gccagataag gcgtttacgc cgcatccggc aattgtgatg gtcagacagc gattaccatt
     7321 tcaccgtcat cgacaaaaac cctgccgtct gggcaaaatc atcactccct ttctgccacg
     7381 ccacgctgcc gcgcagggac actcgctgac tgatattgcc cgtgactccc acttttattt
     7441 caccccgtag cttcaccgca tcgtcactga tagtgctgcc atcttcttca atttccgtcg
     7501 catggggatc gtgataataa ttcagatcta atgttggtgt gacaccaacc gcggtacgcc
     7561 attcgctgtg taaacccagc cgcgtctgaa tatcatcccc ctgcgattgt gacacgcgcg
     7621 catggttagc ggcggtaaaa tcatcctgct gcacgccctg ataaatcacc tgcgcctgcg
     7681 gttcaatcac cacaccacgc cccggtaacc actgataccc ggcttccagc gaggcgataa
     7741 tccccgacga atggtaacga tccacgccat cttcatgttc ggaaacatca ttgttaaacc
     7801 acgcgtattg cagccagcta tccagccagg ccccctgctt ctgattaccg tgctgatacc
     7861 agcttgaggt cagcccaacg gcataaccgt ggttctggtt atcggcgcga gttccggtca
     7921 tattcgagcg gctgtcgccc tgattatcac tgtagccacc aaccgcccca agcatccact
     7981 cgccatccgt gccccagcgc ccgctaaaca agccgccgct aagctgcacc gtagaagtgt
     8041 cttcctgctg agccagttgc cctgccgccg tgtaatgata acgtccgccg ataacacgta
     8101 aattcagcgt ctgaccatcg ccaccagcgt gatcgcgtcg ctccatcata aacgcctaat
     8161 ttgccgcccg caggttattg agataaccgc caactttggc attcagcacc ggctggtaag
     8221 caggcgtagg ggccgggtcg ggggttgggt caggatccgg cgtgggatca ggatcgggag
     8281 tcgggtctgg ctcaggtggc gatggcggcg ttacttcttg cgatcgcaga caccagtcgt
     8341 tgttatcttc cactagcgtg tagtcatacg ctcccatgtt gacgtagcca ctgccagcca
     8401 gactgaactg tgcattgttt tgaaactgcg tgggatcagc tgcgaaatca accactttaa
     8461 tgcctgtcga tgtcggctca ccaatccctg taatgaagtt aaccaccaca gttgtgttgc
     8521 cagcagtatt accgttcatc accaattgat cgcttaccga gtcatcgccg tttaattcgc
     8581 tgtcgaatag tagcgtaccg ccgccggtat aatcaccgtt taccgtcagc gtatcgccag
     8641 cgacgccatt ttgcaggcta accgcaccgg acgtattggt cagatcacca ttaaccgtca
     8701 cgtcagcaag ataggtcagg ctgtcagttt caaggttggt attaagctcg cctgtcaacg
     8761 tcacgccatc ggtagcattt accagcgtcc catcgccgtt aaatagtgag gcattaaggg
     8821 cgacggaagt atcctgcccg gtcagacgca aaaccgtacc atcgctgatg tcgatagtgc
     8881 cgctggaagt agcatcaatt gactgaatat cctgatccgc gccagtaacg aacgttgccc
     8941 catcaccaac ccacagtgac gaagcataag gaaagatatc cgcaacatcg cctttcaggg
     9001 tgccttcttc gacacgtacc gccgactgcg ttgtaccgct ggcggtcagc tccagcgtac
     9061 ccgcccccgt tttagtaaat gtgctaccct catcctgatg ctgcccgctg ctgtcagcca
     9121 tcagtgcgcc ccactgcgtg tctacccccg catcaactgc cacttcaccg tcggcgcgca
     9181 tttcaatatc acgcccatgc tcagtcgtcg tagagcgagt atcgacattg gcagtaatct
     9241 ccatcacgct ttcttgttgc ttatcggtaa agataacctg gcggttatag tgggtatctc
     9301 caagctgcga gttgtcgctc accatcagcg taccggaggc gatttgtgtt gtgcccaaat
     9361 aactattatt gtttgccaga cttacctgcc cgtcgctcgc atcgaccacc acgtagacgc
     9421 catctttccc gtcgccgccg atatgcaaat tgtcgccacc tgcaagatct accacatcct
     9481 gactgccgat aaccgttccg ctgctgccac tgacttcaag gccatcattg tatgatgtgc
     9541 cgctctgcca ggtggagaaa tcagagagat cgagcacacc gccgtttaac acgatcgact
     9601 gcggatcgtc ctggagagcg gtaagatctg ccgcgtcgcc ttccagcgaa agcaccgcac
     9661 catcatcaac gactatatcg ccggttagcg ccatcgactg cgcccctgcc agcacgtagc
     9721 tgccgttttg cgcaatggtg agctgaccag caccttcgat gatgccagca aaactgccct
     9781 gattaacagt aacgttgcca ccagcatcga tatttaaagt gccattctga aagcccgtca
     9841 atgcgtgcac aaaagtttgt tgggtcgagc caacgtttag ctcagcctga ttctgatact
     9901 gatcaatact ccctatcgtc agaccgtagc agtcttgcgg atcgtcctgg caatgcgtat
     9961 cgccgacatt catcagggag ttgctgcgac ccagggtaac ttcaccgttt tcaatctgca
    10021 tctcaccagt aaagtcattg ttatctgcat taagtaccag atcgccggaa cctgttttgg
    10081 tgattaaccc ggtaccagca atagagtcaa cagctccgtc attctctgta ttgccgataa
    10141 ccagcgtttt tccgtcggca atatcaaagg taacttcact taagccgaga tacataaagc
    10201 cacccgccgc agtggaagga ccatctccat agcctgctgc gctattgttc tcatcgacta
    10261 acacgccgcc gttctggctg tagctgtcat caacagaaat atcaataaga tagggagccg
    10321 tcgcgctatt ggtatatatc gccccgccat aaccttcggc tgtgttattt gtaaaggcgg
    10381 tgttatttat tatcgtataa cctgaaggat gcttgctgtc gctattatta tcggtaacat
    10441 cgattgcccc gccatcgcca tcactgtaac ttgttgatgt atatgcttgg ttattgttaa
    10501 aaacatcatc acttaaatag acgtcattat taatggtata aattgcgcca cctttgccat
    10561 cattagcgat attattgcga aacatggcgt tagtgacacg taaatcgacg gcaccagtat
    10621 cattagtacc agaagaatag attgcgccac catagccgcc tgcgacgtta ccggaaaaaa
    10681 taacatcagt aagattcagc gttgagtttt ctttagcaaa tatggccccg ccgttattat
    10741 attctcctgt aacggtgtta ttggcaaaca gagtcattcc agtttcattt tctggtaata
    10801 gtgaaaattc cgctccttgt tgcaaaaata cggccccacc gctggcatta ttggtcatat
    10861 ccgaaaaaac cagccattgc ccatcggc
//
LOCUS       DADLSB010000123        10860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.123, whole genome shotgun sequence.
ACCESSION   DADLSB010000123 DADLSB010000000
VERSION     DADLSB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.123"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            83..424
                     /locus_tag="JN331_003072"
     CDS             83..424
                     /locus_tag="JN331_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY7261153.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(415..1341)
                     /locus_tag="JN331_003073"
     CDS             complement(415..1341)
                     /locus_tag="JN331_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7261154.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRGAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1431..2429
                     /locus_tag="JN331_003074"
     CDS             1431..2429
                     /locus_tag="JN331_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY7261155.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIADGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMIVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2426..2644)
                     /locus_tag="JN331_003075"
     CDS             complement(2426..2644)
                     /locus_tag="JN331_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261156.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="JN331_003076"
     CDS             complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="JN331_003076"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY7261157.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JN331_003077"
     CDS             complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JN331_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY7261158.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5948..6709
                     /gene="cysZ"
                     /locus_tag="JN331_003078"
     CDS             5948..6709
                     /gene="cysZ"
                     /locus_tag="JN331_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY7261159.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6894..7865
                     /gene="cysK"
                     /locus_tag="JN331_003079"
     CDS             6894..7865
                     /gene="cysK"
                     /locus_tag="JN331_003079"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY7261160.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8249..8506
                     /gene="ptsH"
                     /locus_tag="JN331_003080"
     CDS             8249..8506
                     /gene="ptsH"
                     /locus_tag="JN331_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY7261161.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8551..10278
                     /gene="ptsI"
                     /locus_tag="JN331_003081"
     CDS             8551..10278
                     /gene="ptsI"
                     /locus_tag="JN331_003081"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY7261162.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLAQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10319..10828
                     /gene="crr"
                     /locus_tag="JN331_003082"
     CDS             10319..10828
                     /gene="crr"
                     /locus_tag="JN331_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY7261163.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2647 a         2802 c         2685 g         2726 t
ORIGIN      
        1 tgcgatttgt aagattaatt tttcttctca agttccttgt tcttctaccg atattacctc
       61 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
      121 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
      181 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
      241 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
      301 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
      361 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
      421 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
      481 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
      541 ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt caggcaatgg
      601 tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
      661 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
      721 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
      781 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
      841 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
      901 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
      961 atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     1021 aagatgcgcg gaaatggtct ggctggcagc gccccgaact tttccgctca gttgttgccc
     1081 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
     1141 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     1201 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
     1261 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     1321 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     1381 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     1441 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     1501 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     1561 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct gacggtggtc
     1621 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     1681 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     1741 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     1801 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
     1861 caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg
     1921 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
     1981 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
     2041 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
     2101 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
     2161 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
     2221 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
     2281 tccccacatc ggtgatcggt atgatagtgc tgcccctgat gattttccat cagatccaat
     2341 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
     2401 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
     2461 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
     2521 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca attaagcgac
     2581 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
     2641 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
     2701 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
     2761 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
     2821 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
     2881 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
     2941 ctgatgacat tgcggttgct ttcttcagca aagaagttgt gaacgtggga tgcaacgaca
     3001 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
     3061 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
     3121 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
     3181 tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
     3241 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
     3301 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
     3361 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
     3421 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
     3481 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
     3541 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
     3601 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
     3661 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
     3721 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
     3781 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
     3841 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
     3901 ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
     3961 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
     4021 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
     4081 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
     4141 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
     4201 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
     4261 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
     4321 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
     4381 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
     4441 cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
     4501 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
     4561 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcaaagc
     4621 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
     4681 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
     4741 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
     4801 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
     4861 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacttgca taaagatagt
     4921 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
     4981 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
     5041 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
     5101 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
     5161 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
     5221 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
     5281 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
     5341 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
     5401 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga
     5461 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
     5521 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
     5581 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatctttctt tacggctggt
     5641 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
     5701 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
     5761 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
     5821 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
     5881 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
     5941 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
     6001 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
     6061 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
     6121 cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
     6181 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
     6241 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
     6301 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
     6361 cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
     6421 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
     6481 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
     6541 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
     6601 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcaac
     6661 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
     6721 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
     6781 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
     6841 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
     6901 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
     6961 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
     7021 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
     7081 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
     7141 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
     7201 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
     7261 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
     7321 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
     7381 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
     7441 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
     7501 ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag attaaacctg
     7561 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
     7621 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
     7681 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga
     7741 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
     7801 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
     7861 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
     7921 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
     7981 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
     8041 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
     8101 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
     8161 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
     8221 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
     8281 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
     8341 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
     8401 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
     8461 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
     8521 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
     8581 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
     8641 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
     8701 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
     8761 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
     8821 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
     8881 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
     8941 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
     9001 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
     9061 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
     9121 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
     9181 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
     9241 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
     9301 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
     9361 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
     9421 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
     9481 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
     9541 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
     9601 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
     9661 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
     9721 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
     9781 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
     9841 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
     9901 atcggcacca atgatttagc gcagtacact ctggcagttg accgtggtaa tgatatgatt
     9961 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    10021 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    10081 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    10141 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    10201 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    10261 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    10321 gggtttgttc gataaactga aatctctggt ttccgacgac aaaaaggata ccggaactat
    10381 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    10441 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    10501 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    10561 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    10621 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    10681 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    10741 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    10801 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg
//
LOCUS       DADLSB010000124        10808 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.124, whole genome shotgun sequence.
ACCESSION   DADLSB010000124 DADLSB010000000
VERSION     DADLSB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.124"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..947)
                     /gene="baeS"
                     /locus_tag="JN331_003083"
     CDS             complement(<1..947)
                     /gene="baeS"
                     /locus_tag="JN331_003083"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY7261164.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDL"
     gene            complement(944..2359)
                     /locus_tag="JN331_003084"
     CDS             complement(944..2359)
                     /locus_tag="JN331_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7261165.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(2360..5437)
                     /gene="mdtC"
                     /locus_tag="JN331_003085"
     CDS             complement(2360..5437)
                     /gene="mdtC"
                     /locus_tag="JN331_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY7261166.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTEQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(5438..8560)
                     /gene="mdtB"
                     /locus_tag="JN331_003086"
     CDS             complement(5438..8560)
                     /gene="mdtB"
                     /locus_tag="JN331_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY7261167.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(8560..9807)
                     /gene="mdtA"
                     /locus_tag="JN331_003087"
     CDS             complement(8560..9807)
                     /gene="mdtA"
                     /locus_tag="JN331_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAY7261168.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            10086..10142
                     /locus_tag="JN331_003088"
     CDS             10086..10142
                     /locus_tag="JN331_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7261169.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            10414..10470
                     /locus_tag="JN331_003089"
     CDS             10414..10470
                     /locus_tag="JN331_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7261170.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            10742..10801
                     /gene="ibsA"
                     /locus_tag="JN331_003090"
     CDS             10742..10801
                     /gene="ibsA"
                     /locus_tag="JN331_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HAY7261171.1"
                     /translation="MMKHVIILVILLVISFQAY"
BASE COUNT         2533 a         3032 c         2965 g         2278 t
ORIGIN      
        1 atcaaatcta ccggtgcttt ttgataggcg agagcgcctt catcagacat cgacaactga
       61 tgaagatcgt caaccagttt ggtcagtgta ccgacctccg cctgtaaaga cgccaccgtc
      121 tccggcgtga atttgcgcac gccatcctga atggcttcca gttcaccgcg cagcaccgct
      181 aatggcgtac gcagttcgtg agaaatatcg gccataaaat cgcgccgcat ttgctggttt
      241 ttctccagcg tgctggcgag ctggttgaag tcttgcgcca gtttgcccag ttcatcttca
      301 ctggtgggcg ttacgcgggt agtgaaatcg cccgccgcca gtttgtgcgt gccatcgaca
      361 agtcgtttta ccggtgccag caaaccgcgc gccagcagaa aggtggcgag cgccgcgagt
      421 aacgttgcca gggcgacgat cagccagctg gtttgccgct gttgtttatc gaaattgata
      481 tcagtattgc gggtcagccg ctcaacaggg gaggcaatca ccgcgcccac ttccgcaccg
      541 ttgaccagaa tgggtcgccg tgtaccgtca ggtggaatcg gcgctcgcgg accaaccagt
      601 actttgttgt tttgatcgac cacccagaac tgggtacgcc agccgtgcgg cggcataccc
      661 gggccgggtt tatcttccga attatcgtgt tcaaatgaac gcaggatctg aaagacaaag
      721 cgatcgttgt tgcgcaggaa gcgccagtta ccatgctgcg catactgctc gccaagcgca
      781 tcacttaaca gctgtaatcg ctgttcattg ccgtgtttga tgtaatcaat aaagccacgc
      841 tcaaaactga tacgcaccgc ccagtgcata ctgatcagca agacaatgca ggtggcgaaa
      901 atcgccagaa acagtttgcc ggtaataccg ggtcgccaga acttcattgc gcgctccttt
      961 ttcgccgcga aatagctaca ttttgatgcg tatcgttcgg cactctggca aagatgaacg
     1021 ccggaagggc gatgataaac gccatgctaa gccaggtgta cataaagacg gtttgtgtgg
     1081 tgccgctgtc gacgctgaca tgctgcgaac caaaaagccc cagcaacagc ccggcgatgg
     1141 tgacgccgat actcatcgac aattgcataa tcatcgacag caggctgttt ccgctgctcg
     1201 ccagattgtc cgggagatct ttcagcgtca gggtgttcat ggaggagaaa cgggtcgagt
     1261 tgaccatccc ttgtaaaaac aggacgaacg gcaaaacgta gtaccagccc aacagagcgg
     1321 tagtcataaa caacagggtg accagcgaca gacccagcgt ggtcgctacc agtacccgac
     1381 gataaccaaa gcgattcacc acctgtacca caattcgctt cattcccatg ctgccaagca
     1441 ccatcgggat catcatcagt ccggcatgaa acggcgagaa accgaggcca atttgcagga
     1501 aaaccggtgt cataaagggc aacatgccac tgccaatacg tccggcaaag ctccccgcca
     1561 ggcccagcga aaaggtacga gtgcggaaca gtttcagact gaacagggcg cggttgttat
     1621 ttctggcgtg cagcagataa agcaccagtg ccaccacgcc aactgcgacc aggccagcaa
     1681 tcgccagcgg cgataaacct gtacctttac tgccgtccag cgccagggtt aataccgcca
     1741 tgccaaccgc cagcaataaa aagccggaaa gatcaaagcg ccgcgtctgc atggtgtagt
     1801 tcggcattaa catcaatgtg gcgatcgcac cgataatccc caccggaatg ttgatcaaaa
     1861 agatccagtg ccacgacgcg tattctacca gcagaccgcc gagcgccgga ccgagcagcg
     1921 gaccgacctg accgggtaac gtgacaaagg tcatcgccgc catatattgc tcgcgcggta
     1981 cgattttcat caccgtcaac ctgccgaccg gcaccatcat cgcaccgcca acgccctgta
     2041 acgcgcgtgc cagcaacagt tcgttcagcg tgccggaaag cgcgcaaaac agtgaaccga
     2101 gagtaaacag cacgatggcg gtaaagaaaa tattgcgcac gccgactttg tccgccagcc
     2161 agccgctggc gggcagcatc accgccacgg tcagcacata agagacaatg accatgtgca
     2221 tatgcaacgg actttccccg aggctttgcg ccattgaggg aagggcggtg tttacgatgg
     2281 tggtgtccag cgactgcata aagaagccga aagccacaat ccataattgc cagcgggtgc
     2341 tgtcgggaag atctgtcatt tactcggtta ccgtttgttt aggtttacgc gaaaaacgca
     2401 gccgcagacg gtcgaaaaag agatacacca ccggcgtggt atacagcgtc aggagctggc
     2461 tcatcaccag accgccgaca atggtgatcc ccaggggttg ccgcagctcc gagccgtcac
     2521 cgcccgacaa taccagcggc agcgcgccaa acagcgccgc cagggtggtc atcataatcg
     2581 ggcgaaaacg cagcagacag gcctggaaaa tagcttcctg cggcgtcagg ttaccgtgcc
     2641 gttgggcttc aagcgcaaaa tcgaccatca taatggcgtt tttcttcacg atgccgatta
     2701 atagcatgat ccctattagg gcgattaggc tgaacggggc attgaacagc tccagcgcca
     2761 acagcgctcc aacgcccgcc gagggcaggg tggagagaat cgtcagcgga tgtacgtaac
     2821 tctcgtaaag gatacccagc acgatataca ccgtggcgat ggcggcgata atcaggatca
     2881 cctgcgagtt catcgtctcc tggaaaacct gctccgtgcc ggcaaaactg ccgcgcaccg
     2941 tcgaaggcac accaagctgg gtcattgcgc gatcgatcgc cgcactggcg tccgagagcg
     3001 attttccggt cggcaggttg aacgagatgg tcgaggccgc cgataatccc tgatgattca
     3061 ccgatagtgg ggcattcgcc ggttgccatt tagcgaaata cgacagcggg atcgctttgc
     3121 cttcgttatt gataacgaac attttttcca gcgcactgat gtcctgggta tagcgcggat
     3181 ccacttccat caccacttta tactggttca tcggctggta aatggtcgag atttgccgct
     3241 gaccgaaggc gttatttaac agactgttgg cggcctgtac gtcgattccc agccgtgcca
     3301 tggtgtcgcg gtcgtaaacc agattcatct ccgcgccgtt atcctgctga tcggagttca
     3361 cgtccgccag ttccggcaac gtcgccagtt ttttgcggat tttcggctcc cattcgcgca
     3421 gtgccgccag gtcgtcggat aacaacgtgt actggtagct ggcgttcgac tgacgcccac
     3481 caacgcgaat atcttgtacc gccatcagga acagattcgc ccccggttct ttcgccagct
     3541 tcacgcgcag acggtcgata atttgctgcg ccgtttcgct gcgttcgtcg cgtggcttga
     3601 gggtgataaa catcatccca ctgttaactc gtgaaccgcc ggtaaagccg gtgacattat
     3661 ccactgccgg atcgtcacgg ataattttca tgaaatcctg caatttgccg cgcatcgcct
     3721 gaaacgaaat actctgatcc gcctgtatcc cacccatcag tacgccggtg tcctgctccg
     3781 ggaagaaggt tttcgggatc gagatataca gccagatatt cagcgcaatg gtgccaagca
     3841 gcaccacgcc caccagacgg gtatgattga gcacccattt cagcgacttg ccgtagcctt
     3901 gttgcagggc taccaacatg cggccaaaac cacgcagtcg cttttgctca cgcggcttgc
     3961 tggcttttag catccagccg cacatcattg gcgttagcgt cagggaaacc agcaacgaaa
     4021 taccaatggc gacagaaagc gtcacggcaa actcgcgtaa cagtcggccc ggcaatccgc
     4081 ccatcaacag cagcggcagg aacaccgcca ccagtgacag actcatcgac agcaccgtaa
     4141 agccgacttc gcgagtacct tgcagcgcgg cctgtagtgg tttcatcccc gcttccagat
     4201 gacgggcaat gttttccagc accacaatgg cgtcatccac cacgaaacca gtagcaatgg
     4261 tgagcgccat cagcgaaaga ttattgagac tgaatccgca caggtacatc gccgcaaacg
     4321 taccaatcag cgaaaccggc accgcaacgg cgggaataat agtggcgcga cccgagcgca
     4381 ggaataaaaa gaccaccaga atcaccagcg ccaccgagat aatcagcgtt tgctcgactt
     4441 cttccagcga ggcgcgaatg gtgggggagc gatcctgggc aatttgcaga tcaatcgccg
     4501 ccggaatggt ctcctgcaac tctggtaatt ttgcccggat gctgtctacc gtctggataa
     4561 tattggcttc cggcagtttg cggatcatca gtaaaatagc cggtttggcg ttggtcatcc
     4621 cggcgttgcg cacatcctgc actgagtcgg tcaccgtcgc cacatcgccc agacgaaccg
     4681 cgccgccgtt gttgtagtga ataatcagcg gctgatattc agcggcggtt ttcagttcat
     4741 cgttggtctg gatctgccag cggtgagtgc catcttccag cgccccttgc ggtttacgca
     4801 cgttggcatt gctgatggca gtgcgtacgt cgtccagcga cacgccctga ttaaacagcg
     4861 cctgcgggtt cagcccgacg cgcacggcgg gcagcgagct gccaccgaca tcgacatcac
     4921 cgacaccgtc gatttgcgaa atcgtcggag ccagctgcgt cgaggcgaaa tcgtacagtt
     4981 caccctgcga ataagtatcg gatgtcagcg tgaggatcat aattggcgca tccgacgggt
     5041 tcgctttgcg ataggtcggg cggctgggca tcccgctggg cagcaaactt tgtgcagcgt
     5101 tgatcgccgc ctgcacatca cgcgctgcgc cgttgatatc ccggtcaaaa tcaaactgca
     5161 aaataatgcg cgtgctgccg agcgaactgc tggaggtcat ttcactaacc ccggcaatgc
     5221 gcccaagtga gcgctccagc ggcgtggcaa cggaagacgc cattgtctcc ggcgacgcac
     5281 cgggcagcga ggcgctgacc ataatcaccg gaaaatcgac ctgcggcagc ggggcgaccg
     5341 gcagcatacg gaaacccagt atgccgcaca gggtaatggc gaccgacagt aaaatcgtcg
     5401 ccacagggcg gtaaatgaag agggcaaaaa acttcactta cgcctcctct tcatggcgag
     5461 caaagcgact tttggtccac aatgccaggc ggtcgaacag cagataaatc accggcgtgg
     5521 tgaacagcgt cagcacctgg ctgacaatca gaccgccgac catgccgata cctaacggac
     5581 gacgcagttc cgcgccgacc ccggtactca acatcagcgg cagcgcgcca agcagagccg
     5641 ccagggtggt catcaggatc ggacgaaaac gcaacagaca agcctggtag attgcatcgc
     5701 gcggcgacat gccttgctcg cgctcagcag ccagcgcgaa gtcgatcatc atgatggcgt
     5761 tcttcttcac aataccgatc agcaaaataa tgccgataat cgcaatcaca tccagttcgc
     5821 taccggcaat cattaatgcc agcagcgcgc cgacccctgc ggtgggtagc gtcgagagaa
     5881 tggtgatcgg gtgaataaag ctctcgtaca gaatgccgag cacgatatac atcgccacca
     5941 ccgccgcgac aatcagccag acagtgctgc ccagcgccga ctggaaggcg agggtgctgc
     6001 cctggaattg cgtggtgata tccaccggca gattcagcgt cttttcggtg tccataatcg
     6061 cctgcaccgc atcgcccagc gaatagttat ccggcacgtt aaaggagatg gtcgttaccg
     6121 ggaactgatc cagatggttg atggaaagcg gcgcaaaacg ctgctcaatt ttggcaattg
     6181 agcttaacgg caccacgcca ccgtcgctgc tggtcaggcg aatggtatcc agcgccgcga
     6241 ggcctggggt attttcggta ttgtgctcca gcaccacgcg atactggtta gcctgagtat
     6301 aaatagtgga aatcagtcgc tgaccaaacg cgttgtacag ggcgttatcg acgtccgcca
     6361 tgctgatccc cagacggctg gcgctgtcgc gatcaacatt gacatacgcc accagccctt
     6421 tgtcctgcca gtcgctggag acatcagaaa gctgtggcag ttgctggagt ttttccatca
     6481 actgtggcac ccaggtactg agcgcatcca gtgacgtggc ctgcaaggta aactggtact
     6541 gggtgcggct gacctgagta tcgatggtca ggtcctgcgt tggttgcagg aagagatcga
     6601 cgcccggcac tttatctacc gccgtttgca gacgggcgat gactttttgc acccgatcat
     6661 cacgttcatc caacggtttg aggttgattt gtaaacgtgc actattcagc gacgggttag
     6721 tgccatcaac gccaacaaat gaggtcaggc tttgcactgc cggatcctgc aaaatcacgt
     6781 ccgcgacctg gcgttgtcgc tgggccatat tggcaaagga gctggattgc ggtgcctgca
     6841 aagtgccctg aataatgccg ttgtcctgta ccgggaagaa acctttcgga atgaacaccc
     6901 acagcagcac gctaagcagc agcgtgctga gtgccacgct taaggtcagc cacggatgat
     6961 tcagcacttt cgccagtcca cgaccatagg cggcgattat cctgtcgaac attttttccg
     7021 aggcacggga gaagcggttc tgtttacgca acgactcctg gctgagcatc cgcgcgcaca
     7081 tcatcggtgt cagggtcagc gacaccaccg ctgagatcaa aatcgctacc gccagggtaa
     7141 tagcaaattc gcggaacagt cgcccgacga tatcgcccat aaacagcagt gggatcaaca
     7201 ccgcaatcag tgagaaggtc agcgagataa tggtaaagcc gatttcacct gcgcccttga
     7261 gcgccgccgc caacggtttt tcgccttttt cgatatagcg ggaaatgttt tcgatcacca
     7321 cgatggcgtc atcgaccacg aatccggtgg cgatagttaa cgccatcagt gtcaggttat
     7381 tgattgaaaa atcgagaaac accataaccg cgaaagtgcc gattaacgac agcggtacag
     7441 caacaccggg aatgatggtc gccggaatat tgcgcaaaaa caggtagata atcatgacta
     7501 ccagcgcgat agccatcatc aattcaaact gagtatcatc gacggatgcg cggatattgg
     7561 tggtgcgatc ggaaagcact gtcaccttca ccgatttcgg cagactctca gtgagctgtg
     7621 gcagcatctg ccgaatgctg tcggcggtgg agataatgtt agcaccgggc tggcgctgaa
     7681 cattcatcac aatggcctgt tctttgttcg cccacgcgcc gagccagctg ttttctgcac
     7741 cttgctctac agttgcgaca tcgcccagac gaattggcgc gccgttctgg taggcgatga
     7801 ttagctggcg atactcttcg gcggattgca tctggtcgtt cgcggaaagc gtgaccgcac
     7861 gggaagggcc gtcgaggcta ccttttgccg agttaacgtt agcgccggta atggcggtgc
     7921 gcacggtttc gctggtcagg ccgagggcgg caatcgcctg agcgttaagt ttgacgcgaa
     7981 cagccggacg ctgaccgccg gaaagcgtca ccaggccgac gccggaaatc tgcgagattt
     8041 tctgcgcgac gcgggtttcc accatatctt ccacttgcgt catcggcatg gcggttgagg
     8101 tgacggcgag cgtcatgatc ggcggatctg ccgggttcac tttgctgtaa accggcgggt
     8161 taggcagatc gctcggcaac aagttggtcg cagcgttaat cgcggcctgc acttcctgct
     8221 cggcgacatt gagcggtaat gttagctgga actgcaaagt gataactgac gcaccgccgg
     8281 aactttgcga cgacatctgt ttcaggccag acatctgccc gaactggcgt tcgagcggcg
     8341 cggtaacggc agaggtcatg acatccgggc tggcacctgg gtagagcgtg accacctgaa
     8401 tggtcggata gtccacttcc ggcagcgccg aaacgggcag ggcgcgataa ccgataatcc
     8461 cggcgagtaa gatcgccacc atcagcagcg tggtggcgac aggacgcata ataaacaggc
     8521 gcgacgggcc gcctgtgctg ctcgggggta acacctgcat caggagcgtg ctcctttttt
     8581 cgcgtattcg cggctggtgg ctttctcttc cggagtggtg gcgctctggg cttccaccac
     8641 ttccactttc gccccttcgg tcaggcgatc aatgccgtct gtcaccacgc gatcgcccgc
     8701 agaaatacct gcacggatca ccactttctg actgtcctga atgcccggcg tcaccagatg
     8761 tttgctgacc ttgttttcgc tattcagcac ccagacaaaa tggccttcat tgcccatttg
     8821 cagggcggct gttgggatca ctacggcgtt ttgttcggtg tcgactaaca tgcgcgcgtt
     8881 aacaaactga ttgggaaaca gcgcatcatc ctgattatta aagcgtgctt tcactttaat
     8941 cgtaccggtt gtggcatcga tttggttatc gaggcttaac agcgtgcctt cacttaattt
     9001 cttcgaattg gtgcgatccc aggcttctac catcagcggt tttccggctt tttgcgcctg
     9061 cactacagta gcgatatcgc tttccggcag ggtaaagact aaatcgatag gatgcgtctg
     9121 ggtgatcacc acaattccgg tggtatcacc actggagatt tggttaccaa catcaacttg
     9181 cttgagacca acgcgaccat cgactggtgc ggtgatgcgg ctccagtcga gttgcaactg
     9241 cgcgctggcg acgcttgctt catcagcctt aatggtgcct tcggtttcac tgaccagcgc
     9301 ctgttgggca tccagctcct gacgggagac gagattggtt tttgccagtt gctgataacg
     9361 cgccagatca cggcgggcgt tagcaagcgt ggctttatct tttgccagtt ggccctgggt
     9421 ttgtgctaat gcaactttga actggctggg gtcaatttct gccagtaaat cgcctgcttt
     9481 gacctgctgg ccttcctgga aatgtaacgc catcagttga ccgtccacgc ggctgcgcac
     9541 cgtaacagta ttagcggcgg taatggtgcc aagcccggtg aggtaacgcg gaactgcctg
     9601 ttctacggcg gtcgccgcct gaaccggggc taatgggccg gaacgcatac cacggcgacc
     9661 acccgctggc gattgctgcg cttgtttcgt cgcccctggg gctgcactcc gggagtcatt
     9721 gcggccttgc cagaaccaga atgcggcgat ggcggcgata accaccacga ttacgattac
     9781 ccaacgggat ttataactgc ctttcatcgt taagagtttc tcttcctgaa acgtttcgcg
     9841 gaatgatact agtgtagcca gccaataagg gagaaaaatg gaggaattat gaaacactat
     9901 cgggaatcgt ctgaatggcg ggcacatttg cgagcacgca tccagtaata acacaggaaa
     9961 ctattttatc tacgcgttag cgatagactg catgcatggc gaaaggaggt aagccgccga
    10021 tttcagcggg acgctgaaac gggaaagccc ctcccgagga aggggccata aataaggaaa
    10081 gggtcatgat gaagctactc atcatcgtgg tgctcttagt cattagctgg ccagcttact
    10141 aagactacca gggcggggga aaccccgctc taccctcact cctgaaagta tgccttcacg
    10201 ataagattgt caatctgcag gctttgcagt ctgcgatcct gccagcaaat attctttgcg
    10261 agacgttacg caataatcac actggaaact attttatccg caagtcagcg atagactgca
    10321 ttcagggcga aaggaggtaa gccgacgatt tcagcgggac gctgaaacgg gaaagcccct
    10381 cccgaggaag gggccttaaa taaggaaagg gtcatgatga agctactcat catcgtggtg
    10441 ctcttagtca taagcttccc cgcttactaa gactaccagg gcgggggaaa ccccgctcta
    10501 ccctcactcc tgaaattatg ccttcacgat aagattgtca atccgtaggc tttgtagtct
    10561 gcgatcctgc cagcaaatat tctttgcgag ttgttacgca ataatcacag aggaaagtat
    10621 tttgttcacg cgttagcgat agactgcatt cagggcgaaa ggaggtaagc cgacgatttc
    10681 agcgggacgc tgacacggga aagcccctcc cgaggaaggg gccttaaata aggaaagggt
    10741 tatgatgaag cacgtcatca tactggtgat actcttagtg attagcttcc aggcttacta
    10801 agaacacc
//
LOCUS       DADLSB010000125        10763 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.125, whole genome shotgun sequence.
ACCESSION   DADLSB010000125 DADLSB010000000
VERSION     DADLSB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.125"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            283..3150
                     /locus_tag="JN331_003091"
     CDS             283..3150
                     /locus_tag="JN331_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7261172.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3250..5178)
                     /gene="dhaR"
                     /locus_tag="JN331_003092"
     CDS             complement(3250..5178)
                     /gene="dhaR"
                     /locus_tag="JN331_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY7261173.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            5397..6467
                     /gene="dhaK"
                     /locus_tag="JN331_003093"
     CDS             5397..6467
                     /gene="dhaK"
                     /locus_tag="JN331_003093"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY7261174.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYITRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6478..7110
                     /gene="dhaL"
                     /locus_tag="JN331_003094"
     CDS             6478..7110
                     /gene="dhaL"
                     /locus_tag="JN331_003094"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY7261175.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7121..8539
                     /gene="dhaM"
                     /locus_tag="JN331_003095"
     CDS             7121..8539
                     /gene="dhaM"
                     /locus_tag="JN331_003095"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY7261176.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            8859..10556
                     /locus_tag="JN331_003096"
     CDS             8859..10556
                     /locus_tag="JN331_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY7261177.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     VPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMENPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDRKKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
BASE COUNT         2814 a         2704 c         2683 g         2562 t
ORIGIN      
        1 gctgtttgtt gtaatatttt tgccatattc atagtaacga attggaataa gtaacgacaa
       61 cttagggaat aactcgatat aaactcacct ttgtaagggg ggtgttaaat atatattaat
      121 tcctgtgttc ctgattttgc ccggtaatag agaattattc tcagagaagc tcatcatgat
      181 tgcaccatac agatatcatg atgataagag gatattattc attgacttaa acaaaatgac
      241 aaaatccata gacccatagc ctgggtacta tataggagtg gtatgggcat caaacaacac
      301 aatgggaata ccaaagccga tcgtctcgct gaattaaaaa tccgttcgcc ctcaattcaa
      361 ctgataaaat ttggcgctat tggtttgaat gcaattatct tttcccccct gctgatagct
      421 gctgatacag gaagtcaata tggcaccaat attactatta atgatggtga cagaattaca
      481 ggagataccg ccgatccatc aggaaacctc tatggtgtaa tgaccccagc aggaaacacg
      541 cctggcaata tcaacctggg taatgatgtc accgtcaatg tcaacgacgc ctctggatat
      601 gcaaaaggaa tcattattca gggcaaaaac agctccctga cagctaaccg actcacagta
      661 gatgttgttg gtcaaacctc tgccatcggc attaacttaa ttggtgacta tacccatgct
      721 gacttaggca caggcagcac cattaagagt aacgatgacg gcatcattat tgggcatagc
      781 tcaacactaa cagccactca attcaccatt gaaaactcga acggtatagg cctaaccatc
      841 aatgactatg gcaccagtgt cgatcttgga agcggaagta aaatcaagac cgatggaagt
      901 acaggtgttt atatcggtgg tctcaacggc aataacgcca atggtgctgc gcgttttacg
      961 gcgacagacc tgacaatcga tgttcagggc tacagcgcca tggggataaa cgtacagaaa
     1021 aactctgttg tcgatctcgg aacaaacagt accattaaaa ccaatggcga taatgctcac
     1081 ggcctctgga gctttggcca ggttagcgcg aatgcactca ctgttgatgt aactggagcc
     1141 gcggccaatg gcgtcgaagt tcgtggtggt acaaccacta tcggtgcaga tagccatatt
     1201 tcttccgcgc agggcggtgg cctcgtcacc agtggttcag acgcgacaat caatttttct
     1261 ggcacggcag cgcaacgaaa cagcatcttt tccggcggtt cttatggtgc ctcggcccag
     1321 acggcaacgg ctgttatcaa catgcaaaat accgatatta cggttgatcg taatggcagt
     1381 ctggcgctgg gtttgtgggc gctcagcggc ggtagaataa ccggagacag tttggctatc
     1441 accggcgcgg caggagccag aggtatttat gccatgacca acagccagat cgacctcacg
     1501 agcgatctgg tcattgatat gagtacaccc gaccagatgg ccatcgcaac gcaacatgac
     1561 gatggttatg ccgccagccg catcaacgcc tcgggtcgta tgcttatcaa cggtagcgtt
     1621 ctttccaaag gtgggctaat caatctggat atgcaccctg ggtcggtttg gacaggttcc
     1681 tccctcagcg ataatgtcaa tggcgggaaa ctggacgttg caatgaataa cagcgtctgg
     1741 aacgtaacaa gtaattctaa tctcgacacg ctggcgctga gccattcaac tgtcgatttt
     1801 gccagccacg ggtcaactgc cggcacattt accacattaa acgtagagaa cctgagcggt
     1861 aacagtacct ttattatgcg tgctgatgtt gttggcgagg gtaatggcgt taataataga
     1921 ggggatttat tgaatatcag cgggagtagt gctggtaatc acgtattggc tatccgcaac
     1981 cagggcagcg aggccacaac gggaaatgaa gttctgacag tggtaaaaac cactgacggc
     2041 gcggcctcgt tcagcgcgtc ttctcaggtt gagttggggg gatatctgta cgatgtgcgt
     2101 aaaaatggca ctaactggga gctttacgct tccgggacag ttccggaacc gactcctaat
     2161 cctgaaccca caccagctcc cgctcagcct cccatagtca accccgatcc tacgcctgaa
     2221 cccgctccca cgcctaaacc caccacgacc gcagatgctg gcggcaatta tctcaatgtc
     2281 ggttacttat tgaactatgt tgaaaaccgt acgctgatgc aacggatggg tgacttgcga
     2341 aatcagagta aagacggtaa tatctggttg cgcagttatg ggggaagctt ggactccttt
     2401 gccagtggca aactgagcgg ctttgacatg ggttacagcg gtatccagtt tggtggggat
     2461 aaacgtctct ctgatgtaat gccgttgtat gtcggtctgt atattggctc aacacatgca
     2521 tcgccggact atagcggagg cgacggtacc gcacgttcag actacatggg aatgtacgcc
     2581 agttacatgg cacaaaacgg tttttacagc gatctcgtta taaaagcatc gcgccagaaa
     2641 aatagtttcc acgtactgga cagtcagaac aacggcgtta acgccaacgg cactgcgaat
     2701 ggaatgagca tctccctgga agccgggcag aggttcaacc tgtcccctac tggttatggg
     2761 ttctatatag agccgcaaac ccagcttaca tacagccacc agaatgagat ggctatgaag
     2821 gcgagtaatg gcctcaatat acatctgaat cactacgaat cgctgctggg gcgtgccagc
     2881 atgatactgg ggtatgacat caccgcaggc aacagccagc tgaatgtcta tgtgaagact
     2941 ggcgctatcc gcgagttttc aggggatacc gaatatctgt tgaacgactc ccgggagaag
     3001 tacagtttca aaggtaatgg ctggaataac ggcgtgggag tcagtgcaca gtataacaaa
     3061 cagcacacat tctatctcga agcggattac acgcagggta acctctttga tcagaagcaa
     3121 gtcaacggag gatatcgctt cagcttttaa gtgcgggata gacaagccct taaacgatgc
     3181 tgaaaattgt tagcgtgcgc cggaatgaaa tacagatcac tctgatgtat cagaaatcgt
     3241 ttctgtcttt catacccggc gcttaaactg ccctgcatca atgccatgtt gcttcatttt
     3301 ccgccacagc gtagtgcggc cgatcccaag taaagccgac atttcctgaa tgcgaccgcc
     3361 tgtgacttgg gctgcgttaa taattgcctc tttttcaact tccgcaaatg acagactggt
     3421 ggaaaggcgg gtggcgctga catcatctgt cgcctgctcg gtaaacagat gttcaggtaa
     3481 atcactgacg cgaatgcgcc cgttatcact actcagagca agattctcga tgacgctgta
     3541 aagttcaaaa tcgttgcctg gccatgcaca agaaaccagg cgagcgaggg catcgtcatc
     3601 aattttcagc cgcgtagaga agcgtttttc aagactgcgt aatttgttat tcaccagcgc
     3661 cggaatgctg ccacgccgca tacgcagagg cgggatggta atttcaaatg catgcagcgc
     3721 gtaatacagc tggcgactaa aacgattttg ttccaccagc attgcgaggt ccgcggtcgt
     3781 tgttgcaatc actttgacat caattggtat taaacgccgc gcatccagtc gcgtgataac
     3841 cccctgcttg ataacctgaa gcaaagcaga ctgtaactcc accgccagat attcaatctt
     3901 ttcaagaaac agcgtgccgc cgtgtgccag ttccagccga ctcagacggc cattttcatt
     3961 gtccgtgcga tcgccaccaa taaattcttc cgccagcgca gcatcaccat ataactcaca
     4021 attgacggcg atataaggac ctgcagcacg ctcgctttca ttatgaattg cctgacttag
     4081 cagtgccttg cccacgccct cttctccaca aagcaggaca ggaaagctac tgcgcgccgc
     4141 ctggcgacca aaatgaatca agcggcgggt ttgcggatcg tcctgtggca tatgagcgaa
     4201 ggtatggctg acttttccta attgactggt cattaactgc cgcatctgtt ccacaggatg
     4261 gagcaacaaa ataaagctgg ttccctgcgt ttctattatc ggtttaaggg ttatcaccgc
     4321 atcaataaac tggtgctggc tttcaaaggt tgcttctacg tgtttgagcg gatgtgcctg
     4381 ttttattgct tgttgcaata cggcgggtaa cgttaagagt tcagtgattg cccgtccctg
     4441 acttgccgtc gcgtcaaggc gcaagacccg cgccgcctgg gcattaataa attgcaaatt
     4501 accctgctcg tcccagctaa tcacgccatc atccatactt tctaacaggg cattaagttg
     4561 atttaaatga cggttagttt cagcgagcaa actgtccgtc agcagtaaat taccgacctc
     4621 gcgggcgatt gccaacgtca acggcaaatc agctgcggta gtttgctcaa ccggacacgc
     4681 cagcgctatt gttcccgtca atcggccctt gctgtcaaac aacggcgttg cacaaaaggc
     4741 ccagttccag agtgcctgtt tgaaatgttg atcggccatc gttttcacgg cctgaccaga
     4801 gatagccgct aacgatagcg cacaagtacc aataattccc tcggcgcaat acgtgccgtc
     4861 attgaatccc agtgcactta gctgctgcaa ggtttgcgga tcgccattac ggctgagaat
     4921 gcaggcggtt tcatcgagga taaacagcgc acactctcgc ggtgccatat attcccaggc
     4981 atcttccagc gcagcctgcc cgagcgtcag catcgctttc ttacgccgat aaatagaagc
     5041 aaatgtcacg ccctgggcct gatgtggtac gttccatgtc tcccgtttca tcagcttatt
     5101 gcatcgctcc caggaggttg caattaaggg agatatgccc cgaccatcgt tgttaaaagc
     5161 gccactcata tcccgcatcc cttatgacaa aaggtgttcc ggctcgcaat tttaagtatt
     5221 aatacacgta aaagtttcaa tatggaacat tcattaaagc atatgtttca ctatgaaaca
     5281 taatttgggc taattttctt ttcccttgcc tgatgcacaa tggattcatc ggcagtcagt
     5341 ggtcgccgtg tcgttgaaca tcatccatgc cctaccgtaa ttgctggagc aaaataatga
     5401 aaaaattgat caatgatgtg caagacgtac tggacgaaca actggcagga ctggcgaaag
     5461 cgcatccatc gctgacactg catcaggatc cggtgtatat cacccgagct gatgcccctg
     5521 ttgcaggaaa agtcgccctg ctgtcgggtg gcggcagcgg acacgagccg atgcactgtg
     5581 gttatatcgg tcaggggatg ctttcggggg cctgtccggg cgaaattttc acctcaccga
     5641 cgcccgataa aatctttgaa tgctccatgc aaattgatgg cggcgaaggt gtactgttga
     5701 ttatcaaaaa ttacaccggc gatattctta actttgaaac agcgaccgag ttactgcacg
     5761 atagcggcgt aaaagtgacc actgtggtca ttgatgacga cgttgcggta aaagacagtc
     5821 tttataccgc cgggcgacgc ggcgttgcca acaccgtatt aattgaaaaa ctcgtaggcg
     5881 cagcggcaga gcgtggcgac tcactggacg cctgtgcgga actggggcgt aagttgaata
     5941 atcaaggcca ctcaataggt atcgctctcg gtgcctgtac cgttcctgcc gcgggcaaac
     6001 cttcttttac ccttgcggat aatgagatgg agtttggcgt cggcattcat ggtgagccag
     6061 gtattgaccg ccgccccttc tcttcccttg atcaaaccgt cgatgaaatg ttcgacaccc
     6121 tgctggaaca tggctcatac catcgcactt tgcgtttctg ggattatcaa caaggcagct
     6181 ggcaggaaga accacaaacc aaacaaccgc tccagtctgg cgatcgggtg attgcactgg
     6241 ttaacaatct tggcgcaact ccgctttctg agctgtacgg cgtctataac cgcctgacca
     6301 cacgttgcca gcaagcggga ttgactatcg aacgtaattt aattggcgcg tactgcacct
     6361 cactggatat gaccggtttc tcaatcacct tactgaaagt tgatgacgaa acgctggctc
     6421 tctgggacgc cccggtccac accccggccc ttaactgggg taaataagga gaaagcaatg
     6481 tcactgagca gaactcaaat tgttaactgg ctcactcgtt gtggcgatat tttcaacacc
     6541 gagagcgagt atcttaccgg gctggatcgc gagattggcg acgctgacca cgggctaaat
     6601 atgaaccgag gctttagcaa agtggtggaa aaactccctg ctatcgcaga taaagatatc
     6661 ggtttcattc tcaaaaatac cggtatgacg ctgctttcca gcgtcggtgg tgccagtggt
     6721 ccgctgttcg gtaccttctt tatccgcgcc gcacaggcga cccaggcacg gcaaagcctg
     6781 acactggaag agctttatca gatgttccgc gatggcgcgg acggcgtaat cagtcgcggg
     6841 aaagccgaac ctggcgataa aaccatgtgt gatgtgtggg tgccggtggt ggaatcgtta
     6901 cgtcagtcca gcgagcaaaa tctctctgtt ccggtggcgc tcgaagctgc cagtagcatc
     6961 gccgaatccg ctgcacaaag tacgattacg atgcaagccc gcaaaggccg cgccagttat
     7021 ctcggtgaac gcagtattgg tcaccaggat cccggcgcga cctcggtgat gtttatgatg
     7081 caaatgttgg cgttagccgc aaaagagtaa ggaattggtg atggtaaacc tggtcatagt
     7141 ttcacatagc agccgactgg gagaaggtgt cggtgaatta gcccgtcaga tgttaatgag
     7201 tgatagttgt aaaatcgcca ttgccgcggg aattgacgat ccacaaaatc ccattggtac
     7261 cgatgccgtc aaagtgatgg aggccatcga atctgttgct gatgccgacc atgtgctggt
     7321 catgatggat atgggtagcg cattattgag tgctgaaact gcgctggaat tgctggctcc
     7381 cgagatcgcc gcaaaagtac gtttgtgtgc tgcgccgttg gtcgaaggta cactggcagc
     7441 aacggtcagc gcggcctcgg gggcggatat cgacaaagtt atctttgacg ctatgcatgc
     7501 gctggaagcc aaacgtgaac aactgggttt accgtcctcc gacactgaaa tctctgacac
     7561 atgtcctccg tacgatgaag aagcccgttc tctggcggtg gtcataaaaa accgtaacgg
     7621 cctgcatgta cgtccggcct cccggctggt ttatacctta tcgacattta atgccgatat
     7681 gttgctggaa aaaaacggca aatgcgtcac accagagagt attaaccaga ttgcgttact
     7741 acaagttcgc tataacgata cgctgcgcct gattgcgaaa gggccagaag ctgaagaggc
     7801 actgatcgct ttccgtcagc tggctgaaga taactttggt gaaacggagg aagtcgctcc
     7861 acctactctg cgtcccgttc tgcctgtttc gggtaaagcc ttttattatc aaccagtttt
     7921 atgtacggta caggcaaaat caaccctgac cgcggaagaa gaacaagatc gattacgcca
     7981 ggctattgac ttcacgttat tagatctgat gacgttaaca gcgaaagcag aagccagcgg
     8041 gcttgacgat attgccgcaa tcttttctgg tcaccataca ctgttagatg atccggaact
     8101 gctggcggcg gcaagcgaac tccttcagca tgaacattgc acggcagaat atgcctggca
     8161 gcaagttctt aaagaactta gccagcaata ccagcaactg gatgatgaat atctacaagc
     8221 tcgctatatt gatgtggacg atcttctgca tcgcaccctg gtccacctga cccaaacgaa
     8281 agaagaactc ccgcagttta actcgccaac tattctactg gcggagaaca tttatccttc
     8341 cacagtactg caactggatc cggcggttgt aaaaggtatc tgccttagcg ccggaagtcc
     8401 ggtatcccac agcgccctaa tcgcccgtga actggggatt ggctggattt gccagcaggg
     8461 tgagaaactg tatgcgatac aaccagaaga aacgctaacg ctggacgtta aaacgcaacg
     8521 tttcaaccgt cagggttaag ccaccgttcg gatggcatcg ttctgatgtc atccggcaat
     8581 ttacccagca ctgatgtttt ttaactacct gttatatttt ttaccaattt atgatgattt
     8641 ttcttcgcta atcgcgaacc aaatgtcgct ggctggctaa taaacagacg ttatttgatc
     8701 actctatttc gccgttattt atccctttaa ttcccttttc taaaatgcct gaccgttcgc
     8761 agaatgagat ttcgatcatg cagctagtgc gatcctgaac taaggttttc tgatacttga
     8821 ataccgtttt attcggtttc gccaaaggag aatgattgat gaaatccccc gcaccttctc
     8881 gcccgcaaaa aatggcgtta attccagcct gtatcttttt gtgtttcgct gcgctatcgg
     8941 tgcaggcaga agaaacaccg gtaacaccac agccgcctga tattttatta gtgccgctgt
     9001 ttaatgatgt gcaaaacgcc aaactttttc cggaccaaaa aacctttgcc gatgccgtgc
     9061 cgaacagcga tccgctgatg atccttgctg attatcggat gcagcaaaac cagagcggat
     9121 ttgatctgcg ccatttcgtt aacgtcaatt tcaccctgcc gaaagaaggc gagaaatatg
     9181 ttccgccaga ggggcagtca ctgcgcgaac atattgacgg actttggccg gtattaacgc
     9241 gttctaccga aaacaccgaa aaatgggatt ctctgttacc gctgccggaa ccttatgtcg
     9301 tgccgggcgg acgttttcgc gaggtatatt actgggacag ttacttcacc atgttaggac
     9361 ttgccgaaag cggtcactgg gataaagtcg cggatatggt ggccaatttt gctcatgaaa
     9421 tagacactta cggtcatatt cccaacggca accgcagtta ctatttaagc cgctcgcaac
     9481 cgcccttctt tgccctgatg gtagagttac tggcgcagca tgaaggcgat gccgcgttga
     9541 agcaatacct gccgcaaatg caaaaagaat atgcttactg gatggacggt gttgaaaacc
     9601 tgcaagccgg acaacaggaa aaacgcgttg taaaacttca ggatggtacc cttctcaacc
     9661 gctactggga cgatcgcgat acgccacgac cagagtcatg ggtggaagat attgccaccg
     9721 ccaaaagcaa tccgaatcga cctgccactg aaatttaccg cgacctgcgc tctgccgctg
     9781 cgtctggctg ggatttcagc tcgcgctgga tggagaaccc gcagcagtta aataccttac
     9841 gcaccaccag catcgtaccg gtcgatctga acagcctgat gtttaaaatg gaaaaaatcc
     9901 tcgcccgcgc cagcaaagct gcgggagata acgcgatggc aaaccagtac gaaacgctgg
     9961 cgaatgcccg tcaaaaaggg atcgaaaaat acctgtggaa cgatcaacaa ggctggtatg
    10021 ccgattacga cctgaaaagt cataaagtgc gcaatcagtt aaccgcggcc gccctgttcc
    10081 cgctgtacgt caatgcggca gcgaaagatc gcgccaacaa aatggcgacg gcgacgaaaa
    10141 cacatctgct gcaacccggc ggcctgaaca ccacgtcggt gaaaagtggg caacaatggg
    10201 atgcgccaaa tggctgggca ccgttacagt gggtcgcgac agaaggatta caaaactacg
    10261 ggcaaaaaga ggtggcgatg gacattagct ggcacttcct gaccaatgtt cagcacacct
    10321 atgaccggaa gaaaaagctg gtggaaaaat atgatgtcag caccaccgga acggggggcg
    10381 gcggtggcga atatccatta caggatggct ttggctggac caatggcgtg acgctgaaaa
    10441 tgctggattt gatctgcccg aaagagcaac cgtgtgacaa tgttccggcg acgcgtccga
    10501 ccgttaagtc agcaacgacg caaccctcaa ccaaagaggc acaacccaca ccttaaccag
    10561 cacttactcc gacaacgtca ttcggcttct ccggatggcg ttgtcggcag ataacaccat
    10621 atctatggtt ttttctacac agacggtttt tcaagtcctg gcgggaagta ttgcgcaaat
    10681 gtttgccata cgtggttgcc gaagatagcg tcatttctac gggtaagtgt ttcactttca
    10741 tcacccggcg atttacgccg acg
//
LOCUS       DADLSB010000126        10713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.126, whole genome shotgun sequence.
ACCESSION   DADLSB010000126 DADLSB010000000
VERSION     DADLSB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.126"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            429..1547
                     /locus_tag="JN331_003097"
     CDS             429..1547
                     /locus_tag="JN331_003097"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7261178.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWNIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1613..1861
                     /locus_tag="JN331_003098"
     CDS             1613..1861
                     /locus_tag="JN331_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY7261179.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="JN331_003099"
     CDS             1926..2294
                     /locus_tag="JN331_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7261180.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKPDQQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="JN331_003100"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="JN331_003100"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY7261181.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDATILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /locus_tag="JN331_003101"
     CDS             3149..4396
                     /locus_tag="JN331_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY7261182.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKDTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="JN331_003102"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="JN331_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY7261183.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCISFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="JN331_003103"
     CDS             complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="JN331_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAY7261184.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9096..10319)
                     /gene="cusB"
                     /locus_tag="JN331_003104"
     CDS             complement(9096..10319)
                     /gene="cusB"
                     /locus_tag="JN331_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HAY7261185.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFEQAEPHAEKMPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRHLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPNKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSGGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10335..10667)
                     /gene="cusF"
                     /locus_tag="JN331_003105"
     CDS             complement(10335..10667)
                     /gene="cusF"
                     /locus_tag="JN331_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAY7261186.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
BASE COUNT         2596 a         2959 c         2607 g         2551 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatg tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgcaccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcactt tatcgccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgcctcctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaactg gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tggaatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt aaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctggg
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag cccgatcaac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 ccgatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccacaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg ggattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 agacaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccagc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc tgatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggagatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgccat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccaccag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 tgaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggtgcggcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctcg gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac agcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt tagttcggca atgccccggc gcatctccgg
     8281 gccaatctgg accttcgcaa catcgcgcag ataaacgggc acgccatttt cactggcttt
     8341 taaaacgatg tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata
     8401 ttctgcttcc gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc
     8461 gctttttact tcagcgagac tgatgccata ctgcgccagg cgctgagggt cgataaccac
     8521 ctgatactct ttcaccacac cgcctaccga cgccacttcc gcaacgtcag gaatggtttt
     8581 cagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa tcggcaagat cgtgcttacc
     8641 gctgcgatcc accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag
     8701 ctcggcgctg actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg
     8761 cgaacgcgcc cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc
     8821 aaactgcgag aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag
     8881 cggataagtc acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat
     8941 aatcacctgc acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca
     9001 ggtgccccag atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga
     9061 gcgacgaata atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca
     9121 gagcgcatcc gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg
     9181 ccggaaacca ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat
     9241 gcctggaaaa cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc
     9301 cgctgttcgt tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg
     9361 gcggtgttga gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg
     9421 acttccagac gcagctgcag cgtgcgggtc gcggcatcca cgccaggtag cagcgtccat
     9481 ttgcggatgg tgagtgtttt attcggtcgc gccggaacgg tgagggtaaa ctgcgaggca
     9541 tctttcacca gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata
     9601 ccctgaattt tcgcgaccac gttatctttg gcgatattca ttcccgcgcg cagatcaaac
     9661 gcggtgatca cgccatcaat gggcgctttg agcgtaaagc gagtctggat tttttgcgtg
     9721 gcgatcaggt ggcgaatatc cgcctccggc attcccgcca gtcgcagccg ctcaagaatg
     9781 ccttcagtct gggtcgccgt accgccggtt tcgcgcagca gtaaatactc actctgcgct
     9841 tccacccagt cgggaatggt caggtcgaga agcggtgtgc ccttttgtac tttatcgccc
     9901 acggtaagcg gatacacctt gtcgataaac ccggcagcgc gagcctgcac aatggcatac
     9961 tgatactcgt tgtaactgac attcgccggg aaactctggg caaaagtcag cggcccgcgc
    10021 gtgacggtag ccgttttcac ccccagattc tgcgtctgag tcgggtcaat gcgcacacca
    10081 gacgcagaac tctcttcatc ggcatatttc ggcaccagat ccatatccat aaacggcgat
    10141 ttacctggtt tatcgaaccg cgtattggga tacatcgggt cgtaccagaa taagatttta
    10201 cgttctgcgg tcggcatttt ttctgcgtgc ggttccgcct gttcaaacca ggtaaaacct
    10261 gccgctgaaa taataccgcc cgcgatcatg ctgccgataa taagcgcgat ttttttcatc
    10321 tcattaaacc tgggttactg gctgacttta atatcctgta ataaagaaag gttgccctgc
    10381 tggacaaaat taaacgccac tttgtcgccg gttttaattt cactcatttt cgtctgcggg
    10441 gtgatggtaa agcgcatggt catctccggc cagttgacgg cagcaatcgg atcgtgatgg
    10501 atggtgattt ttttgctttc cagatcaaca ccttttacca caccggtggc actaatgacc
    10561 tgtggttgtg cttcgctcat ggtttcatga tgatgttcgt tagcctgggc attaaagcca
    10621 ataacggtaa acagactgaa cattgcgact tgcagtgctt ttttcattta ttctctcctg
    10681 gagttaaaaa ttattgctgc caaccgcccc cta
//
LOCUS       DADLSB010000127        10548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.127, whole genome shotgun sequence.
ACCESSION   DADLSB010000127 DADLSB010000000
VERSION     DADLSB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.127"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(75..1103)
                     /locus_tag="JN331_003106"
     CDS             complement(75..1103)
                     /locus_tag="JN331_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY7261187.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGVRVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPATLDDIPVTWVSTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1278..2870
                     /locus_tag="JN331_003107"
     CDS             1278..2870
                     /locus_tag="JN331_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY7261188.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVASAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2880..4052
                     /gene="malY"
                     /locus_tag="JN331_003108"
     CDS             2880..4052
                     /gene="malY"
                     /locus_tag="JN331_003108"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY7261189.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4156..5157
                     /gene="add"
                     /locus_tag="JN331_003109"
     CDS             4156..5157
                     /gene="add"
                     /locus_tag="JN331_003109"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY7261190.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5193..6233)
                     /locus_tag="JN331_003110"
     CDS             complement(5193..6233)
                     /locus_tag="JN331_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7261191.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6476..6601
                     /gene="blr"
                     /locus_tag="JN331_003111"
     CDS             6476..6601
                     /gene="blr"
                     /locus_tag="JN331_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY7261192.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6874..7089
                     /gene="ydgT"
                     /locus_tag="JN331_003112"
     CDS             6874..7089
                     /gene="ydgT"
                     /locus_tag="JN331_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY7261193.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7175..7615
                     /locus_tag="JN331_003113"
     CDS             7175..7615
                     /locus_tag="JN331_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY7261194.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7692..8273
                     /gene="rsxA"
                     /locus_tag="JN331_003114"
     CDS             7692..8273
                     /gene="rsxA"
                     /locus_tag="JN331_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY7261195.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8273..8851
                     /gene="rsxB"
                     /locus_tag="JN331_003115"
     CDS             8273..8851
                     /gene="rsxB"
                     /locus_tag="JN331_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY7261196.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLQIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDESNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8844..>10548
                     /gene="rsxC"
                     /locus_tag="JN331_003116"
     CDS             8844..>10548
                     /gene="rsxC"
                     /locus_tag="JN331_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY7261197.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNDTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KA"
BASE COUNT         2418 a         2732 c         2684 g         2714 t
ORIGIN      
        1 ccgcctgtgt gccacatttt ttatcattaa ccttgaggaa aggacactac caagtatagc
       61 agataaagag aggattattt cgccgcaatg agccgggcgg gaataataag attgcgtgaa
      121 tgcgtctctt catgggtgat tttttgcatc atgcgatccg caagtgtgat accaagttcc
      181 cgcgctggcg tgctgaccca ggtaacaggt atatcatcaa gtgtggctgg tgtcgcatcg
      241 gtaaatgccg ccagcgaaac ctgttgctca aagtaacgat cgactccgct ttccccgctt
      301 tgccgccctg ctttcagcaa accaaaccat gcccccatcg caatagtttc gttatagcaa
      361 accacggcac tgatggtcgg gttatgacgt aaaagcgccg tgatagcttc cgcggcttgc
      421 ttctggctgg aagtgcactc caacacccaa tcgctgtgaa acggcaggcc aaattttagt
      481 agagttgcac aatagccccc cacccgttct gcacgggtta atgaggaact ttgccctccc
      541 agccaggcga tccgctgatg cccattgcga atgagatgct ccgtcaacaa ctgtgcagcc
      601 tgcatgttgt ccgggcgaac cgtatcaaca tcatcaagat aactggcacg ggaagcgaaa
      661 atcaccggga tagctttttc ttctgccatc cgtcgcaggt catcgctgct tcctgcagcc
      721 ccggcaatta ccacaccatc gacaccctga ttcagtaaca gtgaaaaccg ctgtgccagc
      781 tgctcgccgt ctttaccgcc gtgaagcaaa aaaaccatcc gtccctgcgc ttccagagct
      841 tccgtcaatc cggccgtcaa ttcggcgtaa aacggcgcag ataaatcacg gacgatcaaa
      901 ccaatgacgc cgctttgccc gccgcgcagc gccgacgcct ggcgattgcg cacaaatccc
      961 agctcttcaa tggcggcgtt aacgcgtact cctgtggcgg tagagattcg ccctttgcca
     1021 ctaagcacca gcgaaacggt acttaccgac acgcccgcag ccagcgcaac atcatgaatg
     1081 gttatttttt tggcggtagc catgcagatg acctactccc tgattatgtg acagataaaa
     1141 cgttttacct tttatttatc ttatacccgc tattatcgtt gcgtaatgtg atttatgact
     1201 cactaaaatt tgataaaacg ttttatcttc tcgcgcaatt tactgaatcc agattgttct
     1261 ctacgaggag tcgttttatg acggcgaaaa cagcaccgaa agtcacgctg tgggagttct
     1321 tccagcagtt aggcaaaacc ttcatgttac ccgtggcatt attgtcgttc tgcggcatta
     1381 tgctcggcat tggtagttct cttagcagcc atgatgtcat aaccctgatc ccggtcctgg
     1441 gcaaccccgt gttgcaggct atctttacct ggatgagtaa gattggctcg tttgctttta
     1501 gtttcctgcc tgtcatgttc tgtatcgcca tcccgctggg cctggcacgc gaaaataaag
     1561 gcgtagcggc attcgctggc ttcgtcggtt atgcggtaat gaacctcgcg gtaaacttct
     1621 ggttgaccaa taaaggcatt ctgccaacca cagatgccgc cgttctgaaa gccaataaca
     1681 tccagagcat tcttgggatc cagtcgatcg ataccgggat cctcggtgcg gtgatcgccg
     1741 gtattatcgt ctggatgctg catgagcgtt tccataatat ccgcctgccg gatgcgctgg
     1801 cattcttcgg cggtacgcgc ttcgtaccaa ttatctcctc gctggtgatg ggccttgtcg
     1861 gcctggtgat tccattagtc tggccgattt tcgccatggg tattagcggc ttgggccata
     1921 tgataaacag cgcgggtgat ttcggaccga tgctgtttgg taccggtgaa cgtctgctgt
     1981 tgccgtttgg tctgcatcac attctggtgg cattaattcg ctttaccgac gcaggcggca
     2041 cgcaggaagt ctgcggtcaa accgtcagcg gcgcactgac catcttccag gcgcaattga
     2101 gttgcccgac cactcacggt ttttctgaaa gcgtcacgcg tttcctttcg caaggtaaaa
     2161 tgcctgcgtt tctcggcggt ctgccaggtg cagcgttagc tatgtatcac tgcgcgcgcc
     2221 cggaaaatcg ccataaaatt aaaggtctgc tgatttctgg cctgatcgcc tgcgtcgttg
     2281 gcggcactac cgaaccgctg gaattcctgt tcctgttcgt agcgccagtt ctgtatgtca
     2341 tccacgcgct gttaaccggc ctcggcttca ccgtcatgtc tgtgctcggc gtcaccatcg
     2401 gtaataccga cggcaatatc atcgacttcg tggtgttcgg tattttgcat ggtctgtcaa
     2461 ccaagtggta catggtgcca gtggtggcgg caatctggtt tgtcgtttac tacgtcatct
     2521 tccgtttcgc tatcacccgc ttcaatctga aaaccccggg gcgcgatagc gaagttgcca
     2581 gctcaatcga aaaagccgtt gccagtgcgc cgggtaaatc aggttacaac gttcctgcaa
     2641 tcctcgaagc attaggcggt gccgacaata ttgtcagcct cgataactgc attacccgtc
     2701 tgcgtttgtc tgtggaagat atgtcgcttg ttaatgtgca ggcactgaag gacaatcggg
     2761 caattggcgt agtacaactt aatcaacata acctgcaggt tgttatcggg ccacaagttc
     2821 agtcagtaaa agatgaaatg gccggtctga tgcatactgt ccaggcataa ggataagata
     2881 tgttcgattt ttcaaaggtc gtggatcgtc atggcacatg gtgtacacag tgggattatg
     2941 tcgctgaccg tttcggcact gctgacctgt taccgttcac gatttcagac atggattttg
     3001 ccactgcccc ctgcattatc gaggcgctga atcagcgcct gatgcacggc gtatttggct
     3061 acagccgctg gaaaaacgat gagtttctcg cggctattgc ccactggttt tccacccagc
     3121 attacaccgc aattgatacg cagacggtgg tgtatggccc ttctgtcatc tatatggttt
     3181 cagaactgat tcgtcagtgg tctgaaacag gtgaaggcgt ggtgatccac acgcccgcct
     3241 atgacgcatt ttacaaggcc attgaaggta accagcgcac agtaatgccc gttgctttag
     3301 agaagcaggc tgacggctgg ttttgcgata tgggcaagct ggaagccgtt ttggcgaaac
     3361 cagaatgtaa aattatgctc ctgtgtagcc cacagaatcc taccgggaaa gtgtggacgt
     3421 gcgatgagct ggagatcatg gctgacctgt gcgagcgtca tgatgtgcgg gttatttccg
     3481 atgaaatcca tatggatatg gtctggggcg agcagccgca tattccctgg agtaatgtgg
     3541 cgcggggaga ctgggcgttg ctgacgtcgg gatcgaaaag tttcaatatt cccgcgctga
     3601 ccggtgctta cgggattata gaaaatagca gcagccgtga tgcctattta tcggcactga
     3661 aaggacgtga tgggctttct tccccttcgg tactggcgtt aactgcccat atcgccgcct
     3721 atcagcaagg tgcaccgtgg ctggatgcct tacgcgtcta tctgaaagat aacctgacgt
     3781 atatctcaga taaaatgaac gccgcgtttc ctgaactcaa ctggcagatc ccgcaatcca
     3841 cttatctggc ctggcttgat ctacgtccgt tgaatattga cgacaacgcg ttgcaaaaag
     3901 cgcttatcga gcaagaaaaa gtcgcgatca tgccggggta tacctatggt gaagaaggtc
     3961 gtggttttgt ccgtctcaat gccggctgcc cacgttcgaa actggaaaaa ggtgtggctg
     4021 gattaataaa cgccatccgc gctgttcgct aaccccaatt gcgcaacgta aaaaatcgtt
     4081 gcgcaatcgt ggatttttac cctgctttgt ttttataatg gtgcgcactt ttatatccag
     4141 aaaaagagtg cgaccatgat tgataccacc ctgccattaa ctgatatcca tcgccacctt
     4201 gatggcaaca ttcgtcccca gaccattctt gaacttggcc gccagtataa tatctcgctt
     4261 cctgcacaat cccttgaaac actgattccc cacgttcagg tcattgccaa cgaacccgat
     4321 ctggtgagct ttctgaccaa acttgactgg ggcgttaaag ttctcgcctc tcttgatgcc
     4381 tgccgccgcg tggcatttga aaacattgaa gatgcagccc gtaacggcct gcactatgtc
     4441 gagctgcgtt tttcaccagg ctacatggca atggcacata agctgcctgt agcgggtgtt
     4501 gtcgaagcgg tgatcgatgg cgtacgtgaa ggttgccgca cctttggtgt gcaggcgaag
     4561 cttatcggca ttatgagccg aaccttcggc gaagccgcct gtcagcaaga gctggaggcc
     4621 tttttagccc accgtgacca gattaccgca cttgatttag ctggtgatga acttggattc
     4681 ccgggaagtc tgttcctttc tcacttcaac cgcgcgcgtg atgcgggctg gcatattacc
     4741 gtccatgcag gcgaagctgc cgggccggaa agcatctggc aggcgattcg tgaactgggt
     4801 gcggagcgta ttggacatgg cgtaaaagcc attgaagatc gggcgctgat ggattttctc
     4861 gccgagcaac aaattggtat tgaatcctgt ctgacctcca atattcagac cagcaccgta
     4921 gcagagctgg ctgcacatcc gctgaaaacg ttccttgagc atggcattcg tgccagcatt
     4981 aacactgacg atcccggcgt acagggagtg gatatcattc acgaatatac cgttgccgcg
     5041 ccagctgctg ggttatcccg tgagcaaatc cgccaggcac agattaatgg tctggaaatg
     5101 gctttcctca gcgcagagga aaaacgcgca ctgcgagaaa aagtcgccgc gaagtaacaa
     5161 aaatggatgg tgcaaattgc accatccatt tttcacgcaa ggcacaacgt cgcgcgatgt
     5221 ttggcggatt cgatgcccag ctcaatcaac tccattacct ggattgcctg gcttgccgga
     5281 accggatttt caccatcgcc atttaacgca tcacgaatcg ccgcatagta agccggataa
     5341 ttcccaggca ccgtcaacag cgtttcttcg acacgttcct caccttccac gcgggtaagt
     5401 acgccatcac gcatatcgta gccccagtct tcctgcggca gacgctcgcc atttttcaga
     5461 cgttcttcct gtggatcgag gccatatttc acataactgc ctcgggatcc atgcacgata
     5521 taccgtgctg actcagcggc tgccagcatg gtaccgtgta aaatgactcg ccgctgtgga
     5581 taggacaaga tggcgtggaa ataatcggtc gactgcgctc cgggccgtaa ctgtgccaaa
     5641 tctaccgtca tgctgaccgg taaaccaaat agcgtaatgg cctgatcaag aagatgcggt
     5701 gctaaatcgt accagatacc gctgcctggg ccgccctgtt cacgccaacg atcgcgcacc
     5761 tgcggacgga agcggtcaaa atgagactca aagtaagcaa cttcacccag cacgccttcc
     5821 gcgagcaaac cttttagcgt caagaaatcg ctatcccaac gacggttatg gaatacagac
     5881 agcacacgcc ccaggctttt tgccagcgca tccagctctc gcgcttgtga cagtgtcacg
     5941 gtaaagggtt tatcaacgac cacatgttta cccgcctcaa gcgccgcttt ggctaacggg
     6001 aaatgggtat cgttgggtgt aggaatgaca atcaggtcta tgttgggatc gttaaacaga
     6061 tgcttcggct cagagacaac cgtaaccgtt ggccagtcgg cttttacttt tgtttcatca
     6121 ctgctggaga ttaccgccag ttccagcccg ggcgtgcccg caatcagggg cgcatggaag
     6181 gttttgctcg cataaccata cccaatcaac ccaacacgga tgttgtcgct catgtcattg
     6241 cctctcattg aagtatgatg gctatttgac actatccttt acccacgctc aacagtttaa
     6301 taacctgcca gcaataaggg atgttgttta acttaagtca aaaaaatagc gaattttcca
     6361 acgacaaaag ctaaatatcg caaaaacctc agtaaaaatc ttgctggagc tattattgct
     6421 aagtaacatt taccccctga agttaatgga tcaatcaaga gagatgtggg ctgtaatgaa
     6481 tcgtcttatt gaattaacag gttggatcgt tcttgtcgtt tcagtcattc ttcttggcgt
     6541 ggcgagtcac attgacaact atcagccacc tgaacagagt gcttcggtac aacacaagta
     6601 agctctgcac ttgtggagcg acatgctgcc cgtccgggtg catgttttca cttgtcggat
     6661 attaaaccag gaatttatta tcttgttcga tgttgttggt gattgtcagg gatagtaaag
     6721 cgcgttactc ttttcagagc agtttttcat ccctgaatat ttgccatcag cgcattctta
     6781 ttattgtaat aacacaagcg ttcacttttg gtgaaatttt atcatcacta ataataccta
     6841 cccctttggt attaattatt ttatggacct tttatgactg ttcaggacta cttattaaaa
     6901 tttcgcaaaa tcagttcact cgaaagtctg gaaaaactct acgaccatct taattacacc
     6961 ctgacggacg atcaggaact gatcaatatg tatcgtgctg ccgatcaccg tcgcgcagag
     7021 ctggtttctg gcgggcgttt gtttgacctc ggccaggtac cgaagtccgt ctggcactat
     7081 gtccaataaa gaaagtagcg atcggttaca aaagccttat aataacgccc ctaaaattag
     7141 atttgcctgt ttgcgtcgct attctggaga acctatgacc accacaacgc cacaacgtat
     7201 tggaggctgg ttgcttggcc cgttggcctg gcttttagtc gctctgttga gtacgacgct
     7261 ggcgttgttg ctgtataccg ctgcgttatc ttctccgcaa acatttcaaa cgctcggcgg
     7321 acaagcactg acgacacaaa tcttatgggg cgtttctttt attaccgcta tcgccatgtg
     7381 gtattacaca ctgtggttga ccatcgcttt ctttaaacgt cggcgttgtg ttcctaaaca
     7441 ctatattatc tggctgctta tttccgtatt gctggcagta aaagcattcg ctttttcacc
     7501 cgtggaagtt ggcatagccg ttcgccagtt gctgtttact ttgttggcaa ccgcattgat
     7561 cgtcccctat ttcaaacgct cgtcgcgggt aaaagcgacg tttgtgaatc cgtaataacc
     7621 ttacagttaa cctgttgtcg cctgctctgg attaacagat aataggcggc ttttttattt
     7681 caggccgaaa aatgactgac tacctgttac tgtttgtcgg aactgtactg gtcaataact
     7741 ttgtactggt caagtttctc ggtctctgtc cgtttatggg ggtttccaaa aaactggaaa
     7801 ccgcgatggg catggggctg gcaacaacgt ttgtgatgac gctggcgtct atttgcgcct
     7861 ggcttatcga tacgtggatt ttgatcccac ttaatctgat ttacctgcgc accctggcat
     7921 ttattctggt gattgctgtg gtcgtgcagt tcaccgagat ggtggtgcgc aaaaccagcc
     7981 cggtgcttta ccgtctgctg gggatttttt tgccgcttat caccaccaac tgtgcagtgc
     8041 tcggcgtggc gttgctgaat atcaatctcg ggcacaattt cttgcagtcg gcgctgtacg
     8101 gtttttccgc cgctgtcggt ttctcgctgg tgatggtgct cttcgccgcc atccgagaac
     8161 gccttgctgt ggctgatgtc ccggcacctt ttcgcggtaa tgccattgcg ttaattaccg
     8221 caggtcttat gtctctggcc tttatgggct ttagtggttt ggtgaagttg taatgaatgc
     8281 tatctggatt gccgttgccg ccgtgagcct gctgggcctg gcgtttggcg ccattctggg
     8341 ttatgcctcc cgccgttttg cggtggaaga cgatccggtc gttgagaaaa ttgacgaaat
     8401 cttaccgcag agccagtgtg gtcagtgcgg ttatcccggc tgtcgcccct acgcggaagc
     8461 catcagctgt aacggtgaaa aaatcaaccg ttgcgcccca ggtggcgaag ctgtgatgct
     8521 acaaattgcc gagttgctta atgtcgagcc gcagccgctg gatggcgaag cgcaagagct
     8581 aacgcctgcg cggatggtgg cggttattga tgaaagtaac tgtattggct gcactaaatg
     8641 tattcaggct tgtccggtag acgccatcgt tggcgctacc cgtgccatgc atacggtaat
     8701 gagtgatctc tgtacgggct gcaatttatg tgttgatccg tgcccgacgc actgcatctc
     8761 gttgcaaccg gtcgcagaaa cacctgactc ctggaaatgg gatctgaaca ccattcccgt
     8821 gcgtatcatt cccgtggaac accatgctta agttattctc tgcattcaga aaaaataaaa
     8881 tctgggattt caacggcggc atccatccac cggagatgaa aacccagtcc aacgataccc
     8941 ccctgcgcca ggtaccactg gcgcagcgtt ttgttattcc actgaaacag catattggcg
     9001 ctgaaggtga gttgtgcgtt agcgtcggcg ataaagtatt gcgcggccag ccgcttaccc
     9061 gtggtcgcgg caaaatgctg cctgttcacg cgcccacctc gggtaccgtt acggctattg
     9121 cgccccactc tacggctcat ccttcagctt tagctgaatt aagcgtgatt attgatgccg
     9181 atggtgaaga ctgctggatc ccgcgcgacg gctgggtcga ttatcgctct cgcagtcgcg
     9241 aagagttaat cgagcgcata catcagtttg gtgttgccgg gctgggcggt gccggattcc
     9301 cgacaggcgt taaattgcag ggtggtggag ataagattga aacgttgatt atcaacgctg
     9361 ctgagtgcga gccgtacatt accgccgatg accgtttgat gcaggattgc gcggctcagg
     9421 tcgtagaggg tattcgcatt cttgcgcata ttctgcagcc acgcgaaatt cttatcggca
     9481 ttgaagataa caaaccgcag gcgatttcca tgctgcgcgc ggtgctggcg gactctaacg
     9541 atatttctct gcgggtgatt ccaaccaaat atccttctgg cggtgctaaa caattaacct
     9601 acattctgac cgggaagcag gttccacatg gcgggcgttc atccgatatc ggcgtattaa
     9661 tgcaaaacgt cggcactgct tatgcagtga aacgtgccgt tattgatggc gagccgatta
     9721 ccgagcgtgt tgtaaccctg actggcgaag caatcgctcg cccgggcaac gtctgggcac
     9781 ggctggggac gccagtgcgt catttattga atgatgccgg attctgcccc tctgccgatc
     9841 aaatggtgat tatgggtggc ccgctaatgg gctttacctt gccatggctg gatgtcccgg
     9901 tcgtaaagat taccaactgt ctgttggctc cctctgccaa tgaacttggc gaaccacagg
     9961 aagaacaaag ctgcatccgg tgtagcgcct gtgctgacgc ctgccctgtg gatcttttgc
    10021 cgcaacagtt gtactggttc agcaaaggtc agcaacacga taaagctacc acgcataaca
    10081 ttgccgactg cattgaatgt ggggcttgcg cgtgggtttg cccgagcaat attcccttgg
    10141 tgcaatattt ccgtcaggaa aaagctgaaa ttgcggctat tcgtcaggaa gaaaagcgcg
    10201 ccgcagaagc caaagcgcgt ttcgaagcgc gccaggctcg tctggagcgc gaaaaagcgg
    10261 ctcgccttga acgacataag agcgcagccg ttcaacctgc agccaaagat aaagatgcga
    10321 ttgctgccgc tctggcgcgg gtgaaagaga aacaggccca ggctacacag cctattgtga
    10381 ttaaagcggg cgaacgcccg gataacagtg caattattgc agcacgggaa gcccgtaaag
    10441 cgcaagccag agcgaaacag gcagaactgg agcaaactaa cgacgcagca accgttgctg
    10501 aaccacgtaa aactgccgtt gaagcagcta tcgcccgctc caaagcgc
//
LOCUS       DADLSB010000128        10494 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.128, whole genome shotgun sequence.
ACCESSION   DADLSB010000128 DADLSB010000000
VERSION     DADLSB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.128"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            49..579
                     /locus_tag="JN331_003117"
     CDS             49..579
                     /locus_tag="JN331_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7261198.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="JN331_003118"
     CDS             597..1235
                     /locus_tag="JN331_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7261199.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDADKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="JN331_003119"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="JN331_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY7261200.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN331_003120"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN331_003120"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY7261201.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="JN331_003121"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="JN331_003121"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY7261202.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN331_003122"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN331_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY7261203.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="JN331_003123"
     CDS             complement(5552..6613)
                     /locus_tag="JN331_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY7261204.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="JN331_003124"
     CDS             complement(6610..8007)
                     /locus_tag="JN331_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY7261205.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLGQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="JN331_003125"
     CDS             8163..9161
                     /locus_tag="JN331_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY7261206.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10177)
                     /gene="ompG"
                     /locus_tag="JN331_003126"
     CDS             complement(9272..10177)
                     /gene="ompG"
                     /locus_tag="JN331_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY7261207.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10222..>10494)
                     /locus_tag="JN331_003127"
     CDS             complement(10222..>10494)
                     /locus_tag="JN331_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HAY7261208.1"
                     /translation="TQESLHKPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFML
                     YTTVGGHELVVRAGALNDYHAGENITIHFDMTKCHFFDAETEIAIR"
BASE COUNT         2641 a         2811 c         2486 g         2556 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tgccgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagcgtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cccttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgccccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc acgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actccagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac cacgttccac gtttacccgc gctataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgttctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttcattat tgttccttgt attatcctgg
    10201 gggaatgcct tccccctgta tttagcgaat tgctatttcc gtttctgcat caaagaaatg
    10261 acatttcgtc atatcaaaat gaatagtgat attttctcct gcatgataat catttaacgc
    10321 accagcacgg accactaact cgtgcccccc aaccgtggtg tagagcataa attccgcacc
    10381 ggttaattct gccacgctaa ttttggcgga aatgttattt tcctcttgcg cgtccggatg
    10441 aatatcttcc gggcgtattc ccatcacgat gggcttatgc aaactttcct gtgt
//
LOCUS       DADLSB010000129        10467 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.129, whole genome shotgun sequence.
ACCESSION   DADLSB010000129 DADLSB010000000
VERSION     DADLSB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.129"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(43..402)
                     /locus_tag="JN331_003128"
     CDS             complement(43..402)
                     /locus_tag="JN331_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY7261209.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(399..674)
                     /locus_tag="JN331_003129"
     CDS             complement(399..674)
                     /locus_tag="JN331_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY7261210.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(733..1857)
                     /locus_tag="JN331_003130"
     CDS             complement(733..1857)
                     /locus_tag="JN331_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY7261211.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1857..4592)
                     /gene="rbbA"
                     /locus_tag="JN331_003131"
     CDS             complement(1857..4592)
                     /gene="rbbA"
                     /locus_tag="JN331_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY7261212.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4589..5656)
                     /locus_tag="JN331_003132"
     CDS             complement(4589..5656)
                     /locus_tag="JN331_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7261213.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(6022..7644)
                     /locus_tag="JN331_003133"
     CDS             complement(6022..7644)
                     /locus_tag="JN331_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7261214.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPSA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(7906..9495)
                     /locus_tag="JN331_003134"
     CDS             complement(7906..9495)
                     /locus_tag="JN331_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7261215.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            9992..10467
                     /locus_tag="JN331_003135"
                     /pseudo
     CDS             9992..10467
                     /locus_tag="JN331_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
BASE COUNT         2703 a         2587 c         2316 g         2861 t
ORIGIN      
        1 cctgtaaagg cagatattct gcgtaatgta cgtcgctaaa atttataaat gattcaaacg
       61 ctcattcagt gtttcaataa tataggtatt aacagagact tgttgctgcg cagccgcatt
      121 attaaggcgt tcactcagcg attctggata gcgtaacgta aaggtcttaa tcttttctgt
      181 acgcgcatac ggctcaatac ccgcagcttt acaatcctca agatactctc gcagggaaag
      241 ctctccttct ttttgcagcc cctgaatact gtcagagaca aaatcacaat aacctgacaa
      301 gcccaggaat ttgccacgaa atgcattcag ttctggcacg taggtaataa cagctggttg
      361 cccggcaatc tccatagagt ttggcgtctt cagtttgatc atggttttac ccctatactt
      421 agtaaccagt cacgcacgct ttctacagcg cctttatcgg tatctggcga cggatgaggc
      481 cgatggaaac acgcaacgct catatttagt atgaacttgc aacgcgaacc tcttccttcc
      541 ttaatttctc cgcctaatgc tttaaccagt gactctatat cagaccactt gatcccttga
      601 ggaacaggag ttttaaatat ttgatccaat gtattttttt gtttcttccg taaagcctgt
      661 accttggttc tcatggcatg tccttatgac ttcactttat gacatcacag tatgaagtca
      721 agtttccctt ccttacgcca ttgtcccaat cgtcttcctg aatcgcagca gtgcgatggt
      781 gaaaaacgcg ccgccaattg ccatcagcgt caggaactgc ggccagacga tttcgaatcc
      841 ggcaccccgg tagaggatgg cctgcgcgag gctaacaaag tgtgtcgtcg gcatggtcag
      901 cataatgtcc tgcaccatct gcggcatact ttcgcgcggc gtggatccac cggaaagcat
      961 ttgcagcggc agcagcacca gaatcaccag cagccccagt tgcggcattg aacgcgctat
     1021 cgtccccata aaaatgccga ttgacgtggt ggcaaacaga ctgagcgcca cgcccagcat
     1081 aaacagcggg atcgagcctt caatcggtac gcccagtaca cctttcacca tcagcaccag
     1141 cgataatccc gataccacca gcaccaccag ccccatcgac cagatcttcg ccatcatgat
     1201 ctcaaacggc gttatcggca tcaccagtaa atgttccacc gtgccgtgtt cacgctcgcg
     1261 gatcagcgcc gatccggtca atacaatcgc cagcatggta atgttgttga tgatcgccat
     1321 caccccgcca aaccacgcgg gatcaaggtt cgggttaaag cgcatccggg tttccagcga
     1381 taccaacggt tcgctgttat cacggtagcg cgcgacaaag ctgttcactt caccgttgat
     1441 aatattctgg atatacccat tgccggtaaa tgcctggctc atgcgcgtgg catcgacgtt
     1501 cacctgaata tccggctggc gtccggcgag gacatcacgc tgaaaattag gcggaatatt
     1561 tatcgcgaag gtatagcgtc cggcgtccag tccggcatcc atctcatcgg cggtgatcat
     1621 ctccggtggc aaaaaccacg gacgatagaa gctgttaacg atccggttcg ataactgcga
     1681 ttgatccata tcggcaatgg cgatcggcgc gaggttcaac gatcctggcg taacggtcgc
     1741 tgacgaatac accgacaccg taaacgagaa gacaatcagc gtcagcatcg ctttatcacc
     1801 gagcagactg cgcaactctt tgatacccag attaaaaata ttgcgtaaat ggcgcatcat
     1861 ccctcctgtt ttttcagcag caggatgctt aagcccatca ccagcgggat ggctatcagc
     1921 aacgggataa aaagttgcca caaatcagtc agatccagcg ctttcgagaa cgttccgcgg
     1981 gcgatagtca gaaaatgact ggtcgggtaa acctcgccga tccaacgtcc aggcccttcc
     2041 agcgaagcta ccggatcgat catcccggaa aactgtgtcg ccgggatcaa tgtgataatc
     2101 gccgttccga aaatggcggc aatctggctt ttcataaagg tggagatcag cagccccatt
     2161 ccagtggcaa tgatgatata cagcagcgcc gccagggtga gcgtcaggaa actgccttta
     2221 tgcggtacgc caaacacaaa caccgacagg ccgcagagca ggaaaaagtt cagcatcccc
     2281 agcgcgatgt acggcagctg tttgccaagc aaaaattcgc tacgggtggt gggggtcacg
     2341 taaaggttga taatcgaccc tagctctttt tcccgcacca cgctaagggc gcttagcatt
     2401 gacgggatca tcatcagcag aagcgggatc accgccggaa caatcgctgg caggcttttt
     2461 acgtccgggt tatagcgata acgcgtctca atgttcatca gcccgctttg gctggcgggt
     2521 gtcgattgtc ggctcgccac atcctgaagc caactctggt gcattgcctg cacgtaacct
     2581 tttaccgttt cggcacgact gggcatcgct ccgtcgatcc agacgccgag ttccacaggc
     2641 gtaccacgcg cgatatcgcg cccgaaattg ggcgggatct caatggccac cgtgatatcg
     2701 cccgcacgca tccgacgatc aagctcgtca taactggtga gcggcggctg ttcgataaag
     2761 taacgggaac cggagaggtt gagtgtccac gcctggctac tgacggtctg gtcgcggtcg
     2821 agcaccgcaa agcgcaggtt ttccacatcc atactgatgc cgtaacccat tatcagcatc
     2881 aggatcaccg ttcccatcag cgccagcgtc gaacgtactg gatcgcgtcg cagttccagc
     2941 gcttcgcggc ggctgtagct aaacagacgg cgcaggctaa atccctgacg cggcgcgtgg
     3001 gtggtgtcgt gtaccacggg cggcgcttcg gcttcgttgc tctgccctgc cgcttcctgc
     3061 aaataggcga taaatgcctc ttccagactg gcggctccgc gtttctcaac cagttcctgc
     3121 ggtgtaccgc tggcaagcac ttttccggcg tgcatcagtg agatgcggtc gcaacgttcc
     3181 gcttcgttca taaagtgggt ggagatgaag atagtcactt tgtcctggcg cgagagatcg
     3241 accatcaact gccagaacat atccctcgcc accggatcga caccagaagt aggctcatcg
     3301 aggattaaca tctccgggcg atgaatcacc gccaccgcca gcgaaagccg ctggcgaatg
     3361 ccgagcggca atgactccgg cagaacatct tcaacgtcgt tgagcttaaa acgctcgctc
     3421 atttcagcca ctcgcgcggg aatttccgct tccgggatgt gaaacaaacg ggcatgtaac
     3481 tccaggtttt gccgcacggt gagttcgtta tagagcgaaa acgcctgcga catatagccc
     3541 acacgacggc gggtatcgat atcttttgga tcaaccggtt gcccaaatag ccacgcctca
     3601 ccttcgctgg cgggcagcag tccggtaagc attttcatgg tggtggattt accgcagccg
     3661 ttcgaaccaa ggaatccaaa aatctccccg cgtggaatgc ggaaattgac gtgatcaacg
     3721 gcaacgaagg aaccaaaacg catggtcaga tcgcgcgctt cgatggcaat ctctgcgttt
     3781 tcaggttgat acggtgggat cactaccgcc tgatgcgcct ggcgttgcgc ttgcggtaac
     3841 agattaataa atgcttcttc cagcgtggcg ctttgcgttt gctgccgcag ctcttcggcg
     3901 ctgccggttg ccagcacttc tccggcattc atcgctacca gccagtcgaa gcgttcggcc
     3961 tcttccatat aggcggtggc gaccagcacg ctcatattgc tctgccgctg gcgaatactg
     4021 tcgatcagat cccagaactg ggcgcgggag agcgggtcaa cccccgttgt tggctcatca
     4081 aggatcaaca gttccgggtc gtggattaac gcgcagcaca gcccaagttt ttgcttcatc
     4141 ccgccggaga gtttacctgc cggacgatcg cgaaacggtg ctaacccggt gctggtcagc
     4201 agctcattaa ttcgcacttc ccgctccgct ttatcgtgac cgaacaggcg agcgaaaaaa
     4261 tcgacatttt catacaccga caaggtgtgg tagaggtttt tgcccagccc ctgcggcatc
     4321 caggcgatgc gcgggcagac gtctcggcga tgcttcgggt cgtgcatatc gccacccagc
     4381 accaccacat tgccctgctc aatgacgcgg gcaccggaaa tcagtgacaa taaactcgac
     4441 ttcccgacgc catccggacc aatcagcccg accatgcaac gggccggaat atcgagagtg
     4501 atattgttca gcgcaacggt ttttccataa tgctggctca cgcccgccag ttgcgcgaca
     4561 ggcgggacgg gaaccagttc cagatgcgtc attgcggcaa cctcaccacg aggtcgtcag
     4621 gccacggaag ttcttcattc acccgcaccc acgctacgcc cggtaaaccg gttttgacat
     4681 attccagatg ctgctggagt aattccggtg ggatacgcgc tttgacgcgg aacatcagtt
     4741 tcagccgttc atcgctggtt tcgacggttt ttggcgtgaa ctgggcgaca ctggcgacaa
     4801 aactgatggt tgcaggaata cgcagatctg gcgcggcatc gaggatcagc cgggcttcac
     4861 cgcccagttt cagcgtgccc gcctgttcgg ttggcaggaa gaaggtcata tagacgtcgc
     4921 tgagatcgac catattcagc acccgaccgc ctgccgccag cacttcccct ggctcggcaa
     4981 cccgatactg cacgcgtccg tcacgcgggg ctttcagttc actgtcatcg atatctgcgg
     5041 caatgcgccg ttcagtggct tgcgctgctt cgacccgagt ttgcgcctga atgatattgg
     5101 tgcgtgctgc ttctatagcc gctttagaag ccgaaacctg ggctttcgct gattccagcg
     5161 cagctcgggc gctctcagcg gcggcgcggt cgtcatccag ctgttgcgca gaaatagccc
     5221 ctcgttgggc gagcgagcgg gaacgcgtat gacgtttggc aacggagtcc agttcggcct
     5281 ggcgttgatt gaccagcgac tgtgcggcac gagtttcact ttgtcgttgc tccagcaaag
     5341 cctgcgcggc ggcaacggcg ctttgtgcct ctttgatttg cgcgatggct tccagtcgct
     5401 gttcctgcaa cacgcgagta tccatcttcg ccagcacttc gccttcgcga acaaactggc
     5461 cttctttcac cagaatggtg tcgatacgcc cggcaatttt gctggcgata tccacttccg
     5521 tcgcttcaat gcgcccattg ctgacagcaa agccttccgg cacacccgcc gggcgcaaca
     5581 gccaccacgc cacgacggca gccaccgcca gtgccccgac aacccaccac gccagatggc
     5641 gcttactctt atccataatc gacccgccat aatccctgta agcaaacgac gcgtgttaca
     5701 actgccgccg aacgacgttc agcggattct tccatccggg ataagaagga aacgacaaat
     5761 acgaccagcg cgcagagcat tgagcgccaa cggggaaggc ttagtgaaaa cgggtagtaa
     5821 tgttgtcatc atcagcattc ctggccgtaa atgaaacacg catactcata agtgtgatct
     5881 ataaaactgg ctgacaaaac ggtatccatc ctgttggatc gcgtttcacg cctgttcagt
     5941 tgcaggaaca gagcattcct ttttacgttt tatcaacagt ctcaaccaga tagtaacatt
     6001 aatattcatg ttgtctatgg ttcagagctg taacattgtg cgccttctaa tcaaaggcat
     6061 aagtccattt ctgttttttt catgctggta agacttccag ggagtggtta tctctggccc
     6121 tgcactaggg ttaatttggc aattatgcat gctgaacatt cttccagcat gtttactgtc
     6181 acgggcgtta agacctaaaa ctgtccccgt ttcacctatg cgttctctcc caccatttcc
     6241 gttatgtgca acaacttgat ttatacctaa catttgtgaa catgctaata gtgcttcaag
     6301 tttaggtctg taatctgtcg aaacagaaaa ataacgatta aaggatcgac aagagatagc
     6361 ccggctagtt ttaaactttt tgccattaat tatagcatga tgctttttat taataatttc
     6421 cgctaattcg accggatcga gagcattggt catttcatca atagagacag ttatcggtcc
     6481 tataatgtaa cgttttcgct gatcatcaaa agttatccca tgatgatttg ccattatttt
     6541 gtatttgggg tcatagaaac atacaggata atcttttatc ccgtcgtagg tatcctcatc
     6601 aagtggtttc atgcgcgtga agttttgcat atagttacca ttgcaattcg tttcatgatt
     6661 tccagctaat acatgaacat tttcattgat actcattatg cgttttaaca aatcgatgat
     6721 aaattgatcg ccacagatat tactaaatcg atcgccggtt tggtcgccaa gaaacgtaca
     6781 tggcgtgact gcgtctttat taataaccaa ataaggctga agactttcta aaacattatc
     6841 tcttctgttc agtatactga ttattgtatt aacattagtg tcaagttgct cattaaagtt
     6901 ctcccttagc aatacgttga gaacttcatc ttctatatta agcaactggc taaggaaatt
     6961 ttctccttca gccatgattc ccaaatgtcc tgttgcaaaa agcgcgtaca aaactgcacc
     7021 gactgagcca tcggtatcgc caaaataggt cggcctggta cttgtgggat caacaggtga
     7081 acaattggag aaataggttc tgtcagcgaa ctggggtttt ataattgttt cgaatttaat
     7141 atcaaataca ttgtattgcg atatttccca gttccagatg ggtttgtacg actcagtaac
     7201 tgcaacatca ttagcattat catttactgt catattatta agtaaataaa caatgctgca
     7261 ccaattaata tcggtattat caagagggca accgtaattt ttactattaa ttgtcacaat
     7321 aaactgataa cagttattcc tacgtagaat atgagcgcgc atgcccgata gcatactata
     7381 tttttctgaa tgattcgttt gtatggtaaa tgaaattttg tcatcttgaa tagattgttg
     7441 gaaacgttct atttgccaat catcaatttt ttccacaact tcaccgagca ttgtcctggc
     7501 aatacttgat atatcatcac ctataccatt tgcaatatac ccaaacagtt ctttagtgaa
     7561 gttggtaaca tgctcttgca ccatatcatt tgttgcgatt gtcgttgtac tgccattagt
     7621 gcctgccaca gttccaattt tcatctttta cctcatatta ttcaccgcga ggaatacata
     7681 gttaacattt tatcttatga catacacctc atattcgatg ggaaaataat tatattttgc
     7741 acaaatcatt cagccttaaa gcataatatg aaatttctta tctcatagca gagaaagtcc
     7801 ctaaagattg tagaggcgtc atcagtacat acctctacaa tcgattaaga aaacctatga
     7861 aatactggca aaattaaaaa gagataccac agcactcatg aagttttatt gaggttgaaa
     7921 acaactaagc cctgggacac cttttttatc accacgttcg ttaggtgcta tcttccagga
     7981 atttagcccc atggtataat tatcaatttt ttgatgctgg tctaccccta atccataatg
     8041 gccaaatttc tgtttagttt ttttgagcgt ctgtccacat ttgaagatat cttcaggttt
     8101 tggtctgaaa gcagtagtgt tattaaaata attttcatat acaggtgtgg gtgctatagc
     8161 attgcttcta attagatgga gaagatgatc atccatgtgt tgtttatgtt ttttattaaa
     8221 tatatttgct aattcctcag gatcaattgg gtctttcatc tgattaaatg gaacctgcaa
     8281 agcaccgaga caataacatt gattttcttc atttcttatt atcccgtgat gactggttaa
     8341 aactttagtt tctgagttat aattacaaac atcgagcgtt ttaattaagt tatacgtatc
     8401 accggcagag agtttataat tttctagtcg tgcagtatag ttaccattaa aattgatttc
     8461 atgatttcca gccagaacga tgacattttt attaatacgg ctatctttat taccctccat
     8521 atttcgcata gaatttagca aagtaaggat atatttatca ccaaaaatag tactaaatct
     8581 atcgcctgta tgatccccaa gggggataca aggggttatc gcatcgtcct ttaaaaaaac
     8641 atgttgatgc aactcattca atatcatgtc tctcgtattt aataaattgt agagcaattt
     8701 attattttta tgtatgtatg cagatgaagc catgtcttcg tgttttaata attcacataa
     8761 taaactccaa cccttttccc tgatgcctat atgccctgaa acgagcaatg aataaagtac
     8821 agcgcctact cttccatctg tatcgccaaa ataagccgtg ttggcatttt gtgggttaat
     8881 cacaccacat tgtttataaa agtctatttt agaaaacaca gatttaacat agctatcaaa
     8941 cttatcctta ggaaacgtct ctttagagac atcccaacgc cagtcagctt taaaattacc
     9001 gctaagaaga gtaaagaaat tttcaggtat gatagaaagc ccattaactt tataaattat
     9061 gtctgattta tctccggaga aattaataaa ataataacca tccttacact caatcttaac
     9121 tgtaatggga gacgataatt ttatcacatg gttattgtct ggtataattg taaagataaa
     9181 cgtgttagta gtattatcgg caaaactatt atattcttga atatctatag atggctcact
     9241 attgtaaaac catattttct ccatctgaag actatgttca tttaccctcc agtgaccatg
     9301 ttgcaggaaa tagatgatat gcatgccaaa atcgttatct atttgcgaat cattatccag
     9361 tttatcaatt tcaactatct ttgaacgatg tgttttatca accttgctat ggtgttgatg
     9421 attattaact ttatctgaat gcttatcttt atggtttgaa gggatgtact taaatatatt
     9481 gtcaattgct ttcataaata acccgcgata tttttgacct gttagtaaca aataaatgat
     9541 acatgtctga aagacatatt tacgacgata taaaacaata atacttatat gttcgctgat
     9601 tttaagcacc gagactatcc ggtcttttac taaataatgg aaaacaatcc atccctggaa
     9661 gacaattgta aaaaaaaaca acagaataag aattaatctt aggataaata tttatttatc
     9721 atggcctttt gaccgctaaa aaatggcact cggcacagta aaccccgcta tagttatgct
     9781 atgcatttgt ctttaatgat atctcgtcgt tatattaaca ggctgaaatt atcattaatg
     9841 cacatttcaa tattagcagg gatacctgag gagtttaaag tgaacatata tatcgggtag
     9901 cttttcaaat taatcccttt gattataggc ttaatttgca tcgcgttagg tggctttgtg
     9961 ctggaaagtt cagggcaacg tcgcgggtca tgtgctgatt tctctggcgg ccatatgcct
    10021 agcattattc actaccgcat ttattatcat ttcgcagctc acgcgcggcg ttaatacgtt
    10081 ttacaataca ttgttcccca ttattggcta tgcggggtca attatcacca tgatatgggg
    10141 ttgggcactg ttagcaggta atgatgtgat ggcagacgag tttgttgccg gccatgttat
    10201 ttttggcgtt ggtatgattg ccgcatgtgt atcgacggtg gcagcgtcgt ccggtcactt
    10261 tctgctcatt cccaaaaatg cagcggggag caagagcgac ggaacaccgg tacaggctta
    10321 ttcttcatta atcggtaatt gcctcattgc cgttcccgtt ttactcaccc tgctcggttt
    10381 catttggtct attacgctgt tacatagtgc cgacataact ccgcattatg tcgccttggg
    10441 ttaaccgcaa tctgtgcctg tctaatt
//
LOCUS       DADLSB010000130        10442 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.130, whole genome shotgun sequence.
ACCESSION   DADLSB010000130 DADLSB010000000
VERSION     DADLSB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.130"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            75..629
                     /locus_tag="JN331_003136"
     CDS             75..629
                     /locus_tag="JN331_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAY7261216.1"
                     /translation="MAKLTDKQELFAREYLKDLNATQAAIRAGYSEDSAAVQGCKNLI
                     KANIAKRIAELKAERNEEVGIDAAYVLRRLVEIDQMDVLDILLQNGELKPIKDWPKVW
                     RTTLSGMDVVEMVSADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNEVTGADG
                     GPVRTEITKLTPEQAAEVYRKMMG"
     gene            632..2251
                     /locus_tag="JN331_003137"
                     /pseudo
     CDS             632..2251
                     /locus_tag="JN331_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130781.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
     gene            2251..3792
                     /locus_tag="JN331_003138"
     CDS             2251..3792
                     /locus_tag="JN331_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HAY7261217.1"
                     /translation="MWPFKRKEPSAPAVPDKQPEPQPPEINDEVIASVRQKPRREFVR
                     YEPPPGVIPAPVRDAVLAMDATPYDTLNSHYPDFVYGGFPGYPYLALQAQLPEYRRMV
                     SVLAEEMTRKWIKVKAVGEGDDSHASRIAQLTDALEHYNVQDVFRLAIEHDGFFGRGQ
                     IYIDVRSPSGISAWTDPAELGSGLFISDKKIPKGSLLGLRVIEPVWTYPGIYNADNPL
                     SDDFYRPSEWYVMGKTVHASRMIDLISRPVPDMLKPAYNFGGLSLVQIAEPYVDNWLR
                     TRDSVGDMLHSFSLSGIKTDMTQVLSGKRDPNYAKRAELFNRTRDNRGLLMLDKTNEE
                     FFQFNTPLSGLDTLQAQAQEHMFFVSAIPSVKFAGLSPTGLNASSEGEIRVFYDTIAA
                     LATRLLKKPLKKVLDIIQLSEFGDIDPDITFEFEPLHELTREQLANIRKTEAETDQIY
                     ESTGAVTNNEVRERLATAQDSPYSGIDLSGDIEIDDTEIDNTEENPPQGPNADPEADF
                     TQRGD"
     gene            3833..4522
                     /locus_tag="JN331_003139"
     CDS             3833..4522
                     /locus_tag="JN331_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000552018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY7261218.1"
                     /translation="MHNSVLYWLRAEYRKTDLAQDASPVNLMRGAMQQLARRWQKNFD
                     EMALRLARRFAGDVLKNSDASLYTALRDAGFTVPFRMTTEMNTALQASITENVNLIRS
                     IPQQHLPQVETLVMQSVGRGRDLKTLTDELEKRYGITRRRAALIARDQNNKATSVMQA
                     ARQRSVGITEGVWRHSRAGKTWRPSHVKANGKRFDLRKGMFLDGKWVLPGEEINCRCG
                     WEAVIPGLEKK"
     gene            4519..5868
                     /locus_tag="JN331_003140"
     CDS             4519..5868
                     /locus_tag="JN331_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY7261219.1"
                     /translation="MIITEMLAFDRASVRQFDKVGRFQIERSNLSKANVCGYFGYEIP
                     GAEALGLDPKKLYQLYRDPDELRKAVSTFNNIPVLCRHKPDYPGAPAREYRVGTTHAN
                     SEFDGTYLVNGMSIWDNSAIAGIETDEQREISSSYAYVADMTPGTTPDGEPYDGVMRN
                     IVGNHVALVGDGRVGSDCLVMDSLPQELKRMKLSKKEVAVLTALGTYLAPRLAQDAAP
                     GDLLRLMAQYKRPAAIASAVKTAYSERLVQDMDIEPVELAQLMESAEAVPELVGDDDT
                     GLTDESKAFDTDSPMESVLALLSGKVPDDVLEKIKSALSPTTDEAPETKEADVKPDDV
                     KVDKPAMDAAIRLATDQATKRAAENFRAVRVAEAEVRPLIGDVVAMDCAEDVYRTALE
                     QTGIDIQGIHPGAYRSMVKFAVEQKQTAKSSPRVAMDQASASTFAADFPGAKLKRGY"
     gene            5870..6352
                     /locus_tag="JN331_003141"
     CDS             5870..6352
                     /locus_tag="JN331_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261220.1"
                     /translation="MNTFQTHMNQYPAPGIPVAFASANPHASYVAGDGGLITGPDGLV
                     IARFAWVTKGVAANKGAGAPAGFVPRDGQASIVEWLAGDSNTIYPGRECTLMVSGDFW
                     ALTTTTATIGQKVFASLATGEIATGAAGTSMAGFVETWFSVASAAAAKEVIKISTWSK
                     "
     gene            6352..7380
                     /locus_tag="JN331_003142"
     CDS             6352..7380
                     /locus_tag="JN331_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY7261221.1"
                     /translation="MNKFKQHYATASRDYGIILPGAQAYLPKEYAADYGLAMDAQPAL
                     VTAANSGIPAYFTNYVEPELIRVLVTPMKAAQILGETKKGDWTTLSAQFPIAESDGEV
                     SSYGDYSNNGVVSANVNWVPRQSYHFQTFTVWGERELEMYGAARIGWAAELNVASALT
                     LNKFQNKSYFYGIAGLTNYGLLNDPSLSAPITPDTVDGKLKWDDKDGQGVYDDIVKLF
                     KQLVKQTNGHIERTDKMKLCMSPLAEVNLTKTNQYRVNVSDLLAKNFPAMTIETAVEY
                     TSDAGELVQLIAERLGEQDTGYCAFTEKMRAHAVVADTSSWKQKKSAGTWGAIIRQPL
                     AYAQMLGV"
     gene            7384..7731
                     /locus_tag="JN331_003143"
     CDS             7384..7731
                     /locus_tag="JN331_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261222.1"
                     /translation="MAEMVTVGCKLPNGLVLEVGQKRVQVAGWRNNAVKIVGGYGLTQ
                     VDKVFFDAWLAEHGQQPYVKNGVIFAQDKANSAVSQATEQKAVKSGLEPLPQKNPAPG
                     VNRNDEVMGKPQE"
     gene            7737..8195
                     /locus_tag="JN331_003144"
     CDS             7737..8195
                     /locus_tag="JN331_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HAY7261223.1"
                     /translation="MGTVTFDCRMFVSLYPEFSMVTPELLTAVFNQATALYLDNTDDS
                     PVTDLKEREQLLFLLVAHLCALRGLGNGRDGQAGLVGRITSASEGSVSVSVDNSGSND
                     ASWWYLQTPWGADYWQATAPYRSMQYHSGGAPSRYPGHYYRGYGRGVDGK"
     gene            8185..8769
                     /locus_tag="JN331_003145"
     CDS             8185..8769
                     /locus_tag="JN331_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261224.1"
                     /translation="MVNKVTGGKQFRQKLKQVAANLSLGKKLKVGFLEGATYPDGTSV
                     AYIAAVQEFGGRAVIPAREQTLHFRYNEKTGETGHRFVKAGKGNFVQDVVIPEHTVTI
                     PPRPFFRKMIEHKSPEWGEKMATLLRANDFDTATALVCMGEHIKGQLQMSVRDWESPP
                     NAASTARQKGFNNPLIETGHMMDSVDYSVDGGKK"
     gene            8766..9131
                     /locus_tag="JN331_003146"
     CDS             8766..9131
                     /locus_tag="JN331_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261225.1"
                     /translation="MNFHGIVSGAVRRVNPFTDALVYRSRGSTQQADYSRVPEYEDPV
                     SVRVQKQAVTQADLRHLDNLNQQGAFATLYTDGNWCGLNRARQQGGDKFVIGDETWLV
                     IEVPEIWPDWTRVIVCLQV"
     gene            9116..9661
                     /locus_tag="JN331_003147"
     CDS             9116..9661
                     /locus_tag="JN331_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261226.1"
                     /translation="MPSGVTLSVTESALYQALGDYLRGLFSDAGIERTQQNQVPMPQG
                     NFITMTGIDVTGLSTAVVAYSAPAQAGEGSQHITRTTKWRCQLDFYGPHAADNAQVLA
                     TLFRSEFSVRLFRQGGGLISPLYCSEPFNTTFPNGQQQYEPRWTLDIQMQINPVVTTS
                     LMFFDNVITRTTEADNANSTQ"
     gene            9642..>10442
                     /locus_tag="JN331_003148"
     CDS             9642..>10442
                     /locus_tag="JN331_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HAY7261227.1"
                     /translation="MPIPLSKDVQINPGVLAVAGNAVDLNGLLLTSNPLLPVGTVVPF
                     SSPDDVAAYFGALSDEYARAQLYFQGFKNATRTPGQLLFSRFSPAPSAAWLRSGSFKG
                     ATVEQLQKLSGTLMLSINGKSVSIEVNFNGVASFAAAATALQTALTAAVATVVFDTTQ
                     NAFVITVAGAKPGSTTITFGSGSAAELLKMTSNTGAVISRGAPVSDVPDTMAAIKAAS
                     QQWAGFSTVSEVTDEQHLAFSAWANGQGKRYFYVAWTTSGNARVKGSTE"
BASE COUNT         2533 a         2544 c         3027 g         2338 t
ORIGIN      
        1 cttgataatg gcttataccc tacacgggat aacttaactg atatcccttt taacggataa
       61 acggagcaca aataatggca aagctcaccg acaaacaaga gctgtttgcc cgtgagtacc
      121 tgaaagatct caatgccacg caagcagcca tcagggcggg ttacagtgag gattctgccg
      181 ccgtacaagg atgcaaaaac ctaataaaag ctaatatcgc aaagcgcatt gctgagctta
      241 aagccgagcg aaacgaagag gtaggtatag atgccgctta tgttcttcgt agattggttg
      301 aaatcgacca gatggatgtg ctcgacattc tcctgcaaaa cggtgagcta aagcccatta
      361 aagactggcc taaggtatgg cgcacaacgc tatcaggaat ggatgtcgtg gagatggtat
      421 ccgcagatag cgccgcactt ctgaagaaaa tcaaatggcc tgataaggtt aaaaaccttg
      481 agttgcttgg gcgccatgtt tctgttcagg cgtttaaaga caacgtcaaa aatgaagtga
      541 ctggcgctga cggaggacca gtcagaacag aaattaccaa attaacgcct gagcaggccg
      601 cagaggtgta tagaaaaatg atgggctaag tatgccgtta ccattcccct tcgattttaa
      661 acatcctgat taccagatgg tttttgaatg gcggatggaa cgcctacagc gcattcgcca
      721 gaaccctgaa atattgcccg tattgaagca gttttaccga accaatccgg ctcagttcat
      781 catcgactgg ggcatgacaa cggacccgcg taatattgat tatggcctgc cggtgaccat
      841 tccgttttta ctcttcccta agcaggagga gtggatccac tggattatgg aacgctggag
      901 caatcgggag aatggtatta ccgaaaaatc ccgtgaaatg gggctcagtt ggaccgcgat
      961 cggactggcc tgctcgcttt gtctcttcaa caaagaaatg gttatcggtt tcggctcccg
     1021 taaagaggaa tacgtcgaca gcaccggtga cccgaaagca ttgttctgga aggcgcgcaa
     1081 gttcgtggaa actctacctg tagagtttcg cggttcgtgg agcgagaaga agcacgcgcc
     1141 atatatgcgt gttgagtttc ctgaaactgg cgcggttatc aaaggtgagg ctggcgataa
     1201 tatcggtcgt ggtgaccgta cgacccttta ttttgtggat gaggctgctt ttctccagcg
     1261 accattactt attgatgccg cgctttccca gacaacccga tgtcgtatcg atctctcatc
     1321 ggttaacggc atgaacaacc ccttcgcaca gaagcggcac agtggaaaaa tcccggtgtt
     1381 tacgtttcac tggcgtagcg atccgcgtaa agacgatgag tggtatcaca aggagtgcga
     1441 gaaaattgat aacccagtca tcgttgccta ggaactggat cttaattacc aggcatcagc
     1501 agaaggcatc ctgatcccct cagaatgggt acaggctgcg gttgatgcac atatcagact
     1561 gggcattcag cccggcggcc aacggcttgg tgcaatggat gtcgccgacg aagggcggga
     1621 taaaaacgcc tgttcgctcc gttatggcat cctgctgaat gacgtacagg agtggtcagg
     1681 taaggggagt gacatctatg actctgtggt taaggttttt ggtctgtgcg atgactttgg
     1741 tgccgatgag tttcgctttg atgaggacgg attgggtgct ggcgttcgtg gtgatgcgcg
     1801 cgctatcaac gaactgcgtg aagcggaggg catctgtcaa atcaccgcca ctccattccg
     1861 gggcagcggg agcgtgtttc atcctgaaaa tgaagctgtt cccggtgata atggtaaacc
     1921 ggctcgtctg aataaggact tttttgtcaa tgccaaagcc caggggtggt ggcatcttcg
     1981 caaattattc cgcaatacat ttcgtgcact acagggtatg gagtatgacc cggatgagat
     2041 tatttccatc agcagcacaa tggaaaataa agaccggttg ttgatggagc tgtcacaacc
     2101 tacctggtca aaaaatgcca ccggaaaaat tcttgtggat aagcaacctg acggaacgaa
     2161 atctccaaac ctggctgatt ctatgatgat tgcttatgcc ccgatggaaa tgcccatcgt
     2221 aatttctgat gactttatgg agtggatctg atgtggccgt ttaaacgaaa agaaccgtca
     2281 gcgccggcgg taccggacaa acaacctgaa ccacagccac cggaaattaa cgatgaggtg
     2341 atcgcttccg tccggcagaa accacggagg gagtttgttc gctatgagcc accaccgggc
     2401 gttatcccgg caccggttcg cgatgctgtg ctggcaatgg atgcaacacc gtatgacacc
     2461 ctgaacagtc attatcctga ttttgtttat ggtggtttcc cgggctatcc gtatctggca
     2521 cttcaggcgc agttaccgga gtaccggcgt atggtcagtg tgcttgccga ggagatgacc
     2581 cgtaagtgga taaaggttaa ggcggtcgga gaaggggacg acagccacgc gtcacgcata
     2641 gcgcagctta ctgacgcact ggagcactat aacgtacagg acgttttcag actggcgatt
     2701 gagcacgacg gctttttcgg gcgagggcag atttatattg atgtgcgttc gccatcgggt
     2761 atatctgcct ggactgaccc ggcggagctg ggatccggac tgtttatttc cgacaaaaaa
     2821 atcccgaaag gttccctgct ggggcttcgt gttattgaac ccgtctggac gtatccgggt
     2881 atatataacg cggataatcc gctgagtgat gatttttacc gtccgtccga atggtacgta
     2941 atgggaaaaa cggttcacgc cagccgcatg attgatctga tctcccgtcc ggttccggac
     3001 atgctgaagc cggcctacaa ctttggcggc ctgtcactgg ttcagattgc tgagccttat
     3061 gttgataact ggctgcgaac acgcgacagc gtgggcgata tgctgcattc gttttcgctg
     3121 agcgggatta agacggatat gacacaggta ttaagtggta aaagggaccc gaattacgca
     3181 aaacgcgcgg agttgtttaa ccgtacccgt gataaccgcg ggctgctgat gctggacaag
     3241 acgaacgaag agtttttcca gttcaacact cccctgagcg gcctcgacac ccttcaggcg
     3301 caggcacagg aacacatgtt ctttgtcagt gccataccgt ccgtaaaatt cgccgggctg
     3361 agcccaacgg gactgaatgc gtcgagtgag ggtgaaatcc gggtgtttta cgacaccatt
     3421 gctgcacttg ccacccgtct tctgaagaaa ccgctgaaaa aggtgctgga tattatccag
     3481 ttgtctgagt tcggcgatat cgatcctgat atcacgtttg aatttgaacc cctgcatgag
     3541 ctgacgcgag agcaactggc gaatatccgt aaaactgagg cggaaacgga tcagatttat
     3601 gagagcaccg gagcagtgac caataacgaa gtacgcgaac ggctggcgac tgcgcaggac
     3661 agcccgtaca gcggtattga cctgagcggg gacatcgaaa ttgacgacac tgaaattgac
     3721 aacaccgaag aaaatccgcc gcaaggcccg aatgcagacc ctgaggcgga tttcacccaa
     3781 cgcggggatt gaggcctggt accgcagaca actggataat gccgtcagtg ggatgcacaa
     3841 cagtgtactt tactggctgc gggctgagta ccgtaaaaca gacctcgcgc aggatgcgtc
     3901 ccccgttaac ctgatgcgtg gtgccatgca gcaacttgcc cggcgctggc agaaaaattt
     3961 tgacgaaatg gccctgcggc tggcgaggcg gtttgccggt gatgtcctaa aaaacagcga
     4021 tgcgtcactg tacactgcgc tccgtgatgc cgggtttacg gttcctttcc gtatgacaac
     4081 ggagatgaat accgcacttc aggccagcat cacggagaat gtgaacctca ttcgctccat
     4141 cccgcagcaa catctccccc aggtggaaac actggtcatg cagtctgttg gccggggacg
     4201 tgacctgaaa acgctgaccg atgaactgga aaaacgttac ggcatcacac gacggcgcgc
     4261 ggcgctgatt gcccgcgacc agaacaataa ggcaacgtcg gtaatgcagg ccgccagaca
     4321 gcgttcggtg ggtatcactg aaggtgtctg gcggcattcc cgcgcgggta aaacatggcg
     4381 tccgtcgcat gtgaaggcga acggtaaacg gtttgacctg cgaaagggga tgtttctgga
     4441 tggtaagtgg gtactgccgg gcgaagaaat caactgccgg tgtggctggg aggccgttat
     4501 tcccggactg gagaaaaaat gattattacc gaaatgctgg cgtttgaccg ggcatcggta
     4561 aggcagttcg ataaagtcgg tcgcttccag attgagcgca gtaatctcag caaggcgaac
     4621 gtctgcggtt atttcgggta tgaaataccg ggggcggaag cgctgggact cgaccccaaa
     4681 aaactttatc agctttaccg tgaccccgat gaactgcgca aggcagtttc aaccttcaac
     4741 aatattcccg tcctgtgccg acacaaaccc gattatccgg gcgcgcccgc gcgcgagtac
     4801 cgggtgggga cgactcatgc caacagcgag tttgacggta cctatctggt taacggcatg
     4861 tccatctggg acaactccgc catcgcgggg atagaaacgg atgaacaacg ggaaatctca
     4921 tcgtcatatg cctatgtggc agatatgacg ccgggaacca cccccgacgg tgagccgtat
     4981 gacggcgtta tgcggaatat cgtgggaaat catgtggccc tggtcggcga tggccgggta
     5041 gggtcggact gtcttgttat ggactctctc cctcaggagc taaaacgcat gaaactgagt
     5101 aaaaaagaag tggcggtgct taccgcgctg ggaacctatc ttgcgccgcg tctggcgcag
     5161 gatgcggctc ccggggattt gttacgcctg atggcgcagt ataagcgtcc ggcagctatc
     5221 gccagcgcgg taaaaactgc ttacagcgaa cggctggtac aggatatgga tattgaaccg
     5281 gtggagctgg cgcaactgat ggaatcagca gaagccgtac cggagctggt cggggatgat
     5341 gataccgggt taactgacga gtcgaaggca tttgataccg acagcccgat ggaaagtgta
     5401 ctggcgttgc tgtccggcaa agttcctgat gatgtgctgg aaaaaattaa atccgcactg
     5461 tctccgacaa ccgacgaagc ccccgaaaca aaagaggctg atgtgaaacc cgacgatgtg
     5521 aaagtcgata aacctgcgat ggatgcagca atcaggctgg caactgacca ggcaacgaaa
     5581 cgggctgctg aaaatttccg cgccgtccgt gtggctgaag ctgaagtgcg accgctgatt
     5641 ggcgatgtgg tggcgatgga ctgcgccgaa gatgtttacc gtaccgcgct ggaacagacg
     5701 gggatcgata ttcagggtat tcaccccggt gcgtaccgca gcatggtgaa gtttgccgtt
     5761 gagcagaagc agacggccaa atcctctccc cgtgtggcga tggatcaggc cagcgcatcg
     5821 acgtttgcgg cagatttccc cggtgcaaaa ctgaaacgag gttactgata tgaatacttt
     5881 tcagacacac atgaaccagt acccggcacc gggtattccg gtggcgtttg ccagtgctaa
     5941 ccctcatgcc tcgtatgtgg cgggagatgg cgggctgatt accggccctg acgggctggt
     6001 aattgcccgg tttgcctggg taaccaaagg tgttgccgct aataaggggg ccggcgcacc
     6061 ggcgggtttt gttcctcgtg acgggcaggc ttccattgtg gaatggctgg ccggggactc
     6121 gaacaccatt tatccggggc gtgaatgcac cctgatggtg tcgggggatt tctgggcgct
     6181 gaccaccact actgcgacga tcgggcagaa agtatttgcc tcactggcca ccggggagat
     6241 agccacgggg gcggctggca cctcgatggc ggggtttgtc gaaacctggt tttccgttgc
     6301 cagcgctgcg gcggcaaaag aagtcattaa gatcagtacc tggagcaaat aatgaataaa
     6361 tttaaacagc attatgcgac ggcaagtcgc gactacggga ttattcttcc cggagcacag
     6421 gcttatttgc cgaaggagta cgctgccgat tacggactgg cgatggacgc gcagcctgcg
     6481 ctggtcacag cggctaacag cggtatccct gcgtatttca ccaattacgt tgagccagaa
     6541 ctgatccgcg tgctggtgac gccgatgaaa gccgctcaga ttctgggcga aaccaaaaaa
     6601 ggtgactgga cgacactgtc ggcacaattc ccgattgcag aatctgacgg ggaggtgagt
     6661 tcctacgggg attacagcaa caacggggtg gtaagcgcaa acgtcaactg ggtaccgcgc
     6721 cagagctatc acttccagac cttcaccgta tggggcgaac gtgagctgga gatgtacggc
     6781 gcagcccgta ttggctgggc ggcagagctg aacgtggcgt cagcactgac gctgaataag
     6841 ttccagaata agtcctattt ctacggtatt gccggactga cgaactacgg tttgctgaat
     6901 gatccgtcgt tatctgcacc aatcaccccg gataccgttg acggtaaact gaagtgggac
     6961 gacaaggacg gacagggcgt gtatgacgat atcgtgaaac tctttaagca actggtgaaa
     7021 cagactaacg gtcatattga gcgtaccgac aaaatgaaac tgtgcatgtc gccgctggca
     7081 gaggtgaacc tcaccaaaac aaaccagtac agggttaacg tgtccgatct gctggcgaaa
     7141 aatttcccgg cgatgaccat tgaaacggcg gtggaataca cctctgacgc tggcgagctg
     7201 gtacagctta tcgcggagcg tctgggtgaa caggatacag gctattgcgc tttcactgaa
     7261 aaaatgcgcg cccatgcggt agtggcagat acgtcttcct ggaaacagaa aaaatccgct
     7321 ggtacctggg gggcgattat tcgccagccg ctggcgtatg cacaaatgct gggggtgtga
     7381 gttatggctg aaatggtaac agtgggctgc aaactgccga acggtctggt gctggaagtg
     7441 ggtcagaaac gggttcaggt ggcaggctgg cggaataacg cggttaaaat tgtggggggc
     7501 tatggtctga cgcaggtcga taaggtgttt tttgacgcct ggctggcgga gcacggacag
     7561 caaccttatg tgaaaaatgg cgttattttt gcgcaggata aggcgaacag tgcagtctcg
     7621 caggctacgg agcagaaagc cgttaaatcc ggccttgaac cgctgccgca gaaaaatccg
     7681 gctccgggcg ttaaccgcaa tgatgaagtg atgggtaaac ctcaggagta aacgctatgg
     7741 gtacggtgac gtttgactgt cggatgtttg tcagtctgta cccggagttt tccatggtga
     7801 caccggagct actgacggcg gtgtttaacc aggcgaccgc gttgtacctg gataacacgg
     7861 acgacagtcc ggttaccgac ctgaaggagc gggaacagct tctgttcctg ctggttgcgc
     7921 atctgtgcgc attgcggggg ctgggaaacg ggagagacgg gcaggctggt ctggtggggc
     7981 gtatcaccag tgcgtcggaa ggttctgtat cggtatcggt ggataatagc ggcagtaatg
     8041 atgcgtcgtg gtggtatctc cagaccccct ggggtgctga ctactggcag gcgacagcgc
     8101 cataccgctc aatgcagtat cattccgggg gggcaccttc gcgatatccg gggcattatt
     8161 accggggata cgggaggggc gtcgatggta aataaagtca caggtggcaa acagttccgg
     8221 cagaagctga aacaggttgc cgcgaacctt tcattgggta aaaaactcaa agtgggtttt
     8281 cttgagggag caacttaccc tgacggtacg tcagtggctt atattgccgc agttcaggag
     8341 tttggtggaa gagcggtcat acccgcccgt gagcagacgc tccactttcg ctataacgaa
     8401 aaaacgggag aaaccgggca ccgctttgtc aaagccggta aagggaattt tgttcaggat
     8461 gtggttattc ctgagcacac ggtcaccatt cccccccgtc cgttcttccg taagatgatc
     8521 gaacataaaa gtcccgaatg gggcgaaaaa atggcgacct tattacgggc gaatgatttt
     8581 gatactgcga ccgctctggt gtgtatggga gaacatatca aagggcagtt gcagatgtct
     8641 gttcgcgact gggaaagtcc gcccaacgct gcatccactg cccggcaaaa gggttttaac
     8701 aacccgctta ttgaaaccgg acatatgatg gacagcgttg attattctgt agacgggggc
     8761 aaaaaatgaa ctttcacggt attgtttccg gcgcagtacg tcgggtaaat ccctttacag
     8821 acgcgctggt ttatcgttcc cgcgggagta cacagcaggc agattattcc cgcgtacctg
     8881 agtatgaaga tccggtttcc gtcagggtgc aaaagcaggc tgtcacccag gctgatttac
     8941 gtcatcttga taatcttaac cagcagggag ctttcgccac attgtatacc gatggtaact
     9001 ggtgcgggct taaccgtgcc cggcaacagg gaggcgataa atttgttatt ggcgatgaaa
     9061 cgtggctggt gattgaggta ccggaaatct ggcctgactg gacgagggtt attgtatgcc
     9121 ttcaggtgtg accctctccg ttacggaaag cgctctttat caggccctcg gtgattatct
     9181 ccgggggctt ttttctgatg ctgggattga acgaacgcag cagaaccagg tcccgatgcc
     9241 tcaggggaat ttcatcacca tgacgggcat tgatgttacc ggattatcca ctgcggtagt
     9301 ggcatattct gcgccagcgc aggctggtga gggctctcag catatcaccc gtaccacaaa
     9361 atggcgttgc cagcttgatt tctacggccc tcacgcggca gataacgcgc aggtgctggc
     9421 aacgcttttc cggtctgaat tttccgtacg gcttttccgg cagggcggtg ggctgatttc
     9481 cccgctgtat tgctcagagc cctttaacac cacattcccc aacggtcagc aacagtatga
     9541 accgcgctgg acgcttgata ttcagatgca gattaaccct gtggtcacaa cttccctgat
     9601 gttttttgac aacgtgatca cccggacaac ggaggctgat aatgccaatt ccactcagta
     9661 aagatgtaca gataaatccc ggtgtgctgg ctgtggcggg taatgccgtt gatcttaacg
     9721 gcctgctgtt gaccagtaat ccgttacttc cggtcggcac tgtggttccg ttttcctctc
     9781 cggatgatgt ggccgcgtat tttggtgcat tatccgatga gtacgcacgc gctcagcttt
     9841 atttccaggg cttcaaaaat gccaccagga cgccggggca attgttgttt tcccgtttca
     9901 gtcctgcccc atcggcggcc tggttacgta gcggttcgtt taagggcgcg actgttgaac
     9961 agctacaaaa actttccggc acgctgatgc tgagtattaa cgggaaaagc gtcagcattg
    10021 aagtgaattt taacggtgtc gccagcttcg ccgctgctgc aacggcacta cagacagcgc
    10081 tgaccgcagc agtggctaca gtggtattcg ataccacaca gaatgctttt gtcattactg
    10141 tcgccggggc gaaacccggg agtaccacga taacgttcgg cagtggatcg gcagcagaac
    10201 tcctgaagat gaccagtaac accggtgcgg tgatatcccg gggcgcgcct gtatctgatg
    10261 tccctgacac aatggcagcc attaaggccg cttcccagca atgggcgggt ttttccacgg
    10321 tatctgaggt cactgacgag caacacctgg cgttttctgc atgggcaaac gggcagggca
    10381 agcgttactt ttatgtggca tggacaacca gcggcaacgc cagagtaaaa ggcagtactg
    10441 aa
//
LOCUS       DADLSB010000131        10415 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.131, whole genome shotgun sequence.
ACCESSION   DADLSB010000131 DADLSB010000000
VERSION     DADLSB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.131"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="JN331_003149"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="JN331_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY7261228.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="JN331_003150"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="JN331_003150"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY7261229.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPPFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="JN331_003151"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="JN331_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY7261230.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGVGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="JN331_003152"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="JN331_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7261231.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="JN331_003153"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="JN331_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="JN331_003154"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="JN331_003154"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7261232.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="JN331_003155"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="JN331_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY7261233.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9370)
                     /locus_tag="JN331_003156"
                     /pseudo
     CDS             complement(8007..9370)
                     /locus_tag="JN331_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9393..10085)
                     /locus_tag="JN331_003157"
     CDS             complement(9393..10085)
                     /locus_tag="JN331_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY7261234.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLMAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2553 a         2515 c         2753 g         2594 t
ORIGIN      
        1 aagggggggg ctttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctact aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgctg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccactgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtac tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaagct gcggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccaaggtgta cctcctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct
     3001 ttaaaagcga caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 caggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgta ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgct gatgaacagc
     3721 aggctgtgag tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt attgatcctt gatgagccta cccgtggagt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatag acttgtcggt aggctctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggctgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 ggtgaagtgg tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcaccaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgttg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtactggc gatgacagca ttggcgagag cgttcgccag cagctcgcca ctgataatat
     6361 cgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggcgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ctctttcccc
     6481 atcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcgttat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaag ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc agccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgtttggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagctctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttactgtt actgtgtacc gaaacgcatc agccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc taccgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggcata
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagatc agcatccata tcgccattgc cggtgattgc aaagagaact
     8341 gagcgatcat tagcccaata atcaccgtga tccccactaa tgtatggcga tagcccagac
     8401 gacgtaagac ttgggtaacc atcgattttg caataatgga acctaacgct gtcggtgcca
     8461 tcatacagcc ggcaataaac gcctgataac caaatcctac ctgtaacatc aatggcataa
     8521 ggaacggtac acagccggtc cccagacggg ttgcaatatt gcctacgata ccgatcgaga
     8581 aagtgcgggt tttaaataaa tctaatgaaa ttaatgggtt tggcgtacgt cgcgcatgga
     8641 gaatatagag aagcagtaac ccgatgctgg tgacaattac cgtcaaggaa atccagctgg
     8701 cgacaatctt ttccccgaat agctctattc cgcttgaaaa gagaacaagg ctgaggccaa
     8761 acagcaaaaa gccagtgata tcgaatctgc gtcgtgcggt ggtgaaattg ggcatatgtt
     8821 tgcgcgcgta aagaaggccc gcaataccta tggggatatt aattaaaaat atccagtgcc
     8881 aggttgccca ggtgaccagc acgccgccaa gaacggggcc taaaattggc cccaccagac
     8941 ccggcatggc gacaaaattc aataccggaa gaagttcatt acgaggataa gcacgcagta
     9001 aggccagccg agcaacaggc atcatcattg cgccgcctat cccttgaata acccggaaga
     9061 caaccagctg tggtagcgaa ttagaaagtg cgcaggccag agaacccaac gtgaacagac
     9121 tcacggcaag ggtaaaaatg cgacgcgtac cgaagcgatc ggctagccat ccgcttaccg
     9181 gaataagcat cgccaccgtc agcgtataac tgatgatggc tgattgcatc gcgagaggag
     9241 aacgattaag gctatgagcg attgcgggta aggcggtatt cagaatagtg gcatcaagtg
     9301 cctgcatgaa gaaggccatc gccgcgatcc acggcaaacc cgacatacta cgcttctttt
     9361 tatcgctcat tcaatgtcct gttatcgggt tatcacttat caggtgagcg tagcagcgcc
     9421 tgacaagctt taaatgccgc gtcgccatcg ctttggataa tcgcatcgac aatcgcctgg
     9481 tgcagatcca gctttatcac tgtgtcgctg gtaattgacg tgaagtaagt gtgataaacc
     9541 gaatggaata gcgaggcgaa tgaggtcaaa aacggattgg cgctcatttc atagatatgc
     9601 tcatgccagg ccatatcgac ttcgatccag cgttcacggc gaaagttctc ttttaatgcc
     9661 gccatttcgg ccattaacgt attgagatgc gccttctgtt ccgcggtgcc aaccgttgct
     9721 gccagtaggc aggcttgcgg ctccagacag atacgcataa ccagaaagtg atcgatgacc
     9781 tgatgaaagt tctcttctgt catccaccag gtaagcaatt cctgatcaag aaaattccag
     9841 ttcgattgtg gcatgacccg agtaccaatt cgcggtcgcg gtaaaaccat cccttttgcc
     9901 attaacgttt tgaccgcttc gcgtaccgct gtacgactca ctccaaattg ctcgcccagc
     9961 tcaatctcac cgggcaaaat ggtgccgggt tcatattcac cttttaagat ccgttgcgcc
    10021 agcttctcag ccagaacata cgaaaggttt ttctgtgcag ctaactgttg tgcgcttaaa
    10081 ggcattactt atcttccttt ttctttttat tcctccttag tatgccacca ggaagtgtga
    10141 ttacggttgc aaaaacggca aattgcttgt tttatggcac attaacgggg cttttgctga
    10201 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct
    10261 ataatgcgcc accactgaca cggaacaacg gcaaacacgc cgccggggca gcggggttct
    10321 cctgagaatt ccgacagaga aagcaaaaat aaatgcttga ctctgtggcg ggaaagcgta
    10381 ttatgcacac cccgcgccgc tgagaaaaag cgaag
//
LOCUS       DADLSB010000132        10137 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.132, whole genome shotgun sequence.
ACCESSION   DADLSB010000132 DADLSB010000000
VERSION     DADLSB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.132"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN331_003158"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN331_003158"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY7261235.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN331_003159"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN331_003159"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY7261236.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3325)
                     /locus_tag="JN331_003160"
                     /pseudo
     CDS             complement(2698..3325)
                     /locus_tag="JN331_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3455..5215
                     /locus_tag="JN331_003161"
     CDS             3455..5215
                     /locus_tag="JN331_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HAY7261237.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYGKLQRAKAAFWAK"
     gene            5621..6478
                     /gene="focA"
                     /locus_tag="JN331_003162"
     CDS             5621..6478
                     /gene="focA"
                     /locus_tag="JN331_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY7261238.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6533..8815
                     /gene="pflB"
                     /locus_tag="JN331_003163"
     CDS             6533..8815
                     /gene="pflB"
                     /locus_tag="JN331_003163"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7261239.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9007..9747
                     /gene="pflA"
                     /locus_tag="JN331_003164"
     CDS             9007..9747
                     /gene="pflA"
                     /locus_tag="JN331_003164"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY7261240.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9829..>10137)
                     /locus_tag="JN331_003165"
     CDS             complement(9829..>10137)
                     /locus_tag="JN331_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261241.1"
                     /translation="LLKKRTNGSVRYCQYTSYGDGHKLPFNKVRWVALVGGDKESFVQ
                     MGWEKNISDYLKNMCSYLGIEDADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL
                     "
BASE COUNT         2599 a         2540 c         2404 g         2594 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacagac
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatcac attaccaggc
     3061 ggtataacaa cgccagagtg ataaagacga taagtaccgg caccatcggc acatcatcat
     3121 aaaacgccac atctcccgcc gctgaaccca gcgtgagaat gattaacact tcaaacagcg
     3181 acatctgtcg cacaccgcgt cttccggtca ttttgaggaa caaaaaaacc agtacaaagg
     3241 tataaagact acgtagtgca acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca
     3301 tacggtggag atcgaacgcc ttcattttta tgctcttaac gtggtttatc cgctaaaagc
     3361 atagtcaaca gttcatcggc atggcggcgg tgatagcgcc tttgagataa aacccgtatt
     3421 attgcgcgct ttccgtacga ctaaagtgat tttcatgacg caaacattta tccccggcaa
     3481 agatgccgct ctggaagatt ccatcgctcg cttccagcaa aaactttcag acctcggctt
     3541 tcagattgaa gaggcctcct ggctgaatcc cgtgcctaac gtctggtctg tacatattcg
     3601 cgacaaagag tgcgcactgt gttttaccaa cggtaaaggc gcaaccaaga aagcggcgct
     3661 ggcttctgca ctcggtgaat atttcgagcg tctctcaacc aactactttt ttgctgattt
     3721 ctggctgggc gaaaccatcg ccaacggtcc atttgtgcat tatcccaacg aaaaatggtt
     3781 cccactgagc gaaaatgacg atgtgccaga agggctgctc gatgaccgtc tgcgcgcatt
     3841 ttacgatccg gagaatgaat tgaccggcag tatgctgatt gacctacaat ccggtaacga
     3901 agatcgtggt atttgcggtc tgccgtttac gcgccagtcc gacaatcaga ccgtttatat
     3961 tccgatgaat atcattggta acctgtacgt ttctaacggt atgtccgctg gcaatacccg
     4021 caacgaagca cgcgttcagg ggttgtccga agttttcgaa cgctacgtga aaaaccgcat
     4081 tattgctgaa agcatcagct tgccggaaat cccggcagac gtgctggcgc gttacccagc
     4141 ggtggttgaa gcgatcgaaa cactggaaac agaaggtttc ccgatcttcg catatgatgg
     4201 ttcccttggc ggccagtatc cggtgatttg cgtggtactg tttaatcctg caaacggcac
     4261 ctgctttgcc tctttcggtg cgcatcctga ttttggcgta gcactggaac gtaccgtgac
     4321 cgagctgctg caaggtcgtg gcctgaaaga tttggatgtg tttactccgc caaccttcga
     4381 tgatgaagaa gtcgctgaac ataccaacct cgaaacgcac tttatcgatt ccagcggttt
     4441 aatctcctgg gacctgttca agcaggatgc cgattatccg tttgtggact ggaatttctc
     4501 cggcaccacg gaagaagagt ttgctacgct gatggctatc ttcaacaaag aagataaaga
     4561 agtttatatt gccgattacg agcatctggg cgtttatgct tgccgtatta tcgtgcctgg
     4621 catgtccgat atttatccgg ctgaagatct gtggctcgcg aataacagta tgggcagcca
     4681 tttacgtgaa acgattcttt cgctaccagg cagcgagtgg gaaaaagaag attacctgaa
     4741 cctcatcgag caactggatg aagaaggttt tgatgacttt acccgcgtgc gtgagctgtt
     4801 gggtctggcg accgggtcgg ataacggttg gtacaccctg cgtatcggtg aattaaaagc
     4861 catgctggcg ctggctggtg gcgatctgga acaggctctg gtctggaccg aatggacgat
     4921 ggagtttaac tcatcagtat ttagtccgga acgcgccaac tattatcgct gcctgcaaac
     4981 gttgttatta ctggcacagg aagaagatcg ccagccgctg caatatctga atgcgtttgt
     5041 tcgcatgtac ggcgcagatg ccgtagaagc cgccagtgcg gcaatgagcg gcgaagcggc
     5101 gttttacggc ctgcaaccgg tagatagcga tctgcacgcg tttgctgcac atcagtcgtt
     5161 gttgaaggcc tacggaaagc tgcagcgcgc caaagcagca ttctgggcaa aataaaatca
     5221 aatagcctac gcaatgtagg cttaatgatt agtctgagtt atattacggg gcgttttttt
     5281 aatgccccgc tttacatata tttgcattaa taaaataatt gtaattataa ggttaaatat
     5341 cggtaatttg tatttaataa atacgatcga tattgttact ttattcgcct gatgctccct
     5401 tttaattaac tgttttagcg gaggatgcgg aaaaaattca actcatttgt taatttttaa
     5461 aatttatttt tatttggata atcaaatatt tactccgtat ttgcataaag accatgcgag
     5521 ttacgggcct ataagccagg cgagatatga tctatatcaa tttctcatct ataatgcttt
     5581 gttagtatct cgtcgccgac ttaataaaga gagagttagt gtgaaagctg acaacccttt
     5641 tgatctttta cttcctgctg caatggccaa agtggccgaa gaggcgggtg tctataaagc
     5701 aacgaaacat ccgcttaaga ctttctatct ggcgattacc gccggtgttt tcatctcaat
     5761 cgcattcgtc ttctatatca cagcaaccac tggcacaggc acaatgccct tcggcatggc
     5821 aaaactggtt ggcggcattt gcttctctct ggggctgatt ctttgtgttg tctgcggagc
     5881 cgatctcttt acttccacag tgttgattgt tgttgctaag gcgagtgggc gcatcacctg
     5941 gggtcagttg gctaaaaact ggctaaatgt ctattttggc aacctggtcg gcgcactgct
     6001 gtttgtactt ttaatgtggc tttccggcga gtatatgacc gcaaatggtc aatggggact
     6061 aaacgtccta caaaccgccg accacaaagt gcaccatact tttattgagg ccgtctgtct
     6121 tggtatcctg gcaaacctga tggtatgtct ggcagtatgg atgagttatt ctggccgcag
     6181 cctgatggac aaagcgttca ttatggtgct gccggtcgcg atgtttgttg ccagcggttt
     6241 tgagcacagt atcgcaaaca tgtttatgat cccgatgggt attgtaatcc gcgacttcgc
     6301 atctccggaa ttctggaccg ctgtcggttc tgcaccggaa aatttttctc acctgaccgt
     6361 gatgaacttc atcactgata acctgatccc ggttacgatc ggtaatatta tcggcggtgg
     6421 tttgttggtt gggttgacat actgggtcat ttacctgcgt gaaaacgatc accattaatg
     6481 gttgtcgaag tacgcagtaa ataaaaaatc cacttaagaa ggtaggtgtt acatgtccga
     6541 gcttaatgaa aagttagcca cagcctggga aggttttacc aaaggtgact ggcagaatga
     6601 agtaaacgtc cgtgacttca ttcagaaaaa ctacactccg tacgagggtg acgagtcctt
     6661 cctggctggc gctactgaag cgaccaccac cctgtgggac aaagtaatgg aaggcgttaa
     6721 actggaaaac cgcactcacg cgccagttga ttttgacacc gctgttgctt ccaccatcac
     6781 ctctcacgac gctggctaca tcaataaagc gttggaaaaa gttgttggtc tgcagactga
     6841 agctccgctg aaacgtgctc ttatcccgtt cggtggtatc aaaatgatcg aaggttcctg
     6901 caaagcgtac aaccgcgaac tggacccgat gatcaaaaaa atcttcactg aataccgtaa
     6961 aactcacaac cagggcgtgt tcgacgttta cactccggac atcctgcgtt gccgtaaatc
     7021 cggtgttctg accggtctgc cagatgctta tggccgtggc cgtatcatcg gtgactaccg
     7081 tcgcgttgcg ctgtacggta tcgactacct gatgaaagac aaatacgctc agttcacctc
     7141 tctgcaggct gatctggaaa acggcgtaaa cctggaacag actatccgtc tgcgcgaaga
     7201 aatcgctgaa cagcaccgcg ctctgggtca gatgaaagaa atggctgcga aatacggcta
     7261 cgacatctct ggtccggcta ccaacgctca ggaagctatc cagtggactt acttcggcta
     7321 cctggctgct gttaagtctc agaacggtgc tgcaatgtcc ttcggtcgta cctccacctt
     7381 cctggatgtg tacatcgaac gtgacctgaa agctggcaag atcaccgaac aagaagcgca
     7441 ggaaatggtt gaccacctgg tcatgaaact gcgtatggtt cgcttcctgc gtactccgga
     7501 atacgatgaa ctgttctctg gcgacccaat ctgggcaacc gaatctatcg gtggtatggg
     7561 cctcgatggt cgtaccctgg ttaccaaaaa cagcttccgt ttcctgaaca ccctgtacac
     7621 tatggggccg tctccggaac cgaacatgac cattctgtgg tctgaaaaac tgccgctgaa
     7681 cttcaagaaa ttcgccgcta aagtgtccat cgacacctct tctctgcagt atgagaacga
     7741 tgacctgatg cgtccggact tcaacaacga tgactacgct atcgcttgct gcgtaagccc
     7801 gatgatcgtt ggtaaacaaa tgcagttctt cggtgcgcgt gcaaacctgg cgaaaaccat
     7861 gctgtacgca atcaacggcg gcgttgacga aaaactgaaa atgcaggttg gtccgaagtc
     7921 tgaaccgatc aaaggcgatc tcctgagcta cgatgaagtg atggagcgca tggatcactt
     7981 catggactgg ctggctaaac agtacatcac tgcactgaac atcatccact acatgcacga
     8041 caagtacagc tacgaagcct ctctgatggc gctgcacgac cgtgacgtta tccgcaccat
     8101 ggcgtgtggt atcgctggtc tgtccgttgc tgctgactcc ctgtctgcaa tcaaatatgc
     8161 gaaagttaaa ccgattcgtg acgaagacgg tctggctatc gacttcgaaa tcgaaggcga
     8221 atacccgcag tttggtaaca atgatccgcg tgtagatgac ttggctgttg acctggtaga
     8281 acgtttcatg aagaaaattc agaaactgca cacctaccgt gacgctatcc cgactcagtc
     8341 tgttctgacc atcacttcta acgttgtgta tggtaagaaa actggtaaca ccccagacgg
     8401 tcgtcgtgct ggcgcgccgt tcggaccggg tgctaacccg atgcacggtc gtgaccagaa
     8461 aggtgctgta gcgtctctga cttccgttgc taaactgccg tttgcttacg ctaaagatgg
     8521 tatctcctac accttctcta tcgttccgaa cgcactgggt aaagacgacg aagttcgtaa
     8581 gaccaacctg gctggtctga tggatggtta cttccaccac gaagcatcca tcgaaggtgg
     8641 tcagcacctg aacgttaacg tgatgaaccg tgaaatgctg ctcgacgcga tggaaaaccc
     8701 ggaaaaatat ccgcagctga ccatccgtgt atctggctac gcagtacgtt tcaactcgct
     8761 gactaaagaa cagcagcagg acgttattac tcgtaccttc actcaatcta tgtaattaga
     8821 tttgactgaa atcgtacagt aaaaagcgta caataaaggc tccacgaaag tggggccttt
     8881 tttagcacga gagccttttt tgtcagctat ctatacttta aggtgactgc caaaacagac
     8941 tcgacgtagc cttcgagctg cgcaccaaca cggcctcaga tgggccacat ctggagaaac
     9001 accgcaatgt cagttattgg tcgcattcac tcctttgaat cctgtggaac cgtagacggc
     9061 ccaggtattc gctttatcac ctttttccag ggctgcctga tgcgctgcct gtattgtcat
     9121 aaccgcgaca cctgggacac gcatggcggt aaagaagtta ccgttgaaga tttgatgaag
     9181 gaagtggtga cctatcgcca ctttatgaac gcttccggcg gcggcgttac cgcatccggc
     9241 ggtgaagcaa tcctgcaagc tgagtttgtt cgtgactggt tccgcgcctg taaaaaagaa
     9301 ggcattcata cctgtctgga taccaacggt tttgttcgtc gttacgatcc ggtgattgat
     9361 gaactgctgg aagtaaccga cctggtaatg ctcgatctca aacagatgaa cgacgagatc
     9421 caccaaaatc tggttggagt ttccaaccac cgcacgctgg agttcgctaa atatctggcg
     9481 aacaaaaatg tgaaggtgtg gatccgctac gttgttgtcc caggctggtc tgacgatgac
     9541 gattcggcgc atcgcctcgg tgaatttacc cgtgatatgg gtaacgttga gaaaatcgaa
     9601 cttctcccct accacgaact gggcaaacac aaatgggtgg cgatgggtga agaatacaaa
     9661 ctcgatggtg ttaaaccacc gaagaaagag accatggaac gcgtgaaagg cattcttgag
     9721 cagtacggtc ataaggtaat gttctaattt ccccgtaaag cggcaacttt attgagttgc
     9781 cgctttttta ttccgcgctt cattctgcta cagcgttttt aacacatctt atagtgcatc
     9841 taccatatcc cgtacctgag ataataacga ctgataatag ctcgcgtgaa gttcttcccc
     9901 atcgaatacc actgtattac acaagaaagt gacatcggca tcttcaatac caagataact
     9961 acacatattt tttaaataat cgcttatatt tttttcccag cccatctgga caaatgattc
    10021 tttgtctcct ccaaccagcg ccacccaacg aactttattg aatggtaatt tgtgcccatc
    10081 tccatatgag gtgtactggc aatagcggac actaccattt gttctttttt taagcag
//
LOCUS       DADLSB010000133        10088 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.133, whole genome shotgun sequence.
ACCESSION   DADLSB010000133 DADLSB010000000
VERSION     DADLSB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.133"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            61..5301
                     /locus_tag="JN331_003166"
     CDS             61..5301
                     /locus_tag="JN331_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261242.1"
                     /translation="MASTNAHQIKNNDQNSLCGLGDKIRRLTAGVCLFTQIFFPVMAT
                     AQNVVHAKPQTTVSSAPPLLENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTSQKSHEQQNAVPPANGENTLENQIASTSQRVGTLLSQDM
                     NSEQASGMARGWASSEASGAMTDWLNNFGTARISLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTSLGWRHFTSSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDYDYEARPANGWDLRAEGWLPVWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSMQKQLNPDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIRLSLLDPVKGKSGEI
                     KPLVSSLQTKYALKGYNIEAAALKAAGGKVSTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNLSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYIQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPVVSSRDHSSITIDNVSYYAGDDIKVRVELKDDSNQPVAYQKEELVKAVT
                     VENSKPGATIVWHEEQPGVYAANYPAHKQGTALRAQLSLHNWNAPLQSHIYNIEANQN
                     KARVATLSATNNDVYADKKTFNTLTINVTDESDNPLTNHQVTFKNEKGSAEFVEPPQQ
                     NTDGYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTAIVTDFHNNPLKDMKVNFVAPGGSQLDNTTATTDQSGIVRVHLTSSKA
                     GSYSVDASLEVDKNIHQSVTITVVPNREQSVMTLNAGSGSAIANNTNIVTLTASVKDV
                     YGHPLPDEDVKFTLPASMTGNLTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVAYQQVTFIGDTNSAQLQPLTASLNSIVAGNSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSETEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQASDNKTFSLSVLPDESS
                     AKVISITGAEKTITVGKNITLRILVQDAFNNVIAGQRVRLSAQPTTNITIGDTAYTDN
                     NGYAYVNLLSTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNARGELMPGQIITFSVTPEGATLSNTGEVLTDQSGQAKVTLTSDKVNVYTVTA
                     IMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGIDSSTITSRVEDDYGFPVE
                     GVDVSHGLDTKGSPVVNIPTTRTDQSGQVTATITNTLAETLTVNVQVPGTANQSATIT
                     LVAGTADESKSILKSDVDTLKADYQQSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSVSLNLIHSIKEMSG
                     HVTANNHTFSTAKFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQSWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYN
                     LPSITHLTSGENKRTQGSLYGEWGNVGAFSSNSQFTPGAYWTSESDDYSRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(5425..6111)
                     /gene="kptA"
                     /locus_tag="JN331_003167"
     CDS             complement(5425..6111)
                     /gene="kptA"
                     /locus_tag="JN331_003167"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY7261243.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            6258..6852
                     /locus_tag="JN331_003168"
                     /pseudo
     CDS             6258..6852
                     /locus_tag="JN331_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6849..7310
                     /locus_tag="JN331_003169"
     CDS             6849..7310
                     /locus_tag="JN331_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261244.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            7323..8495
                     /gene="iadA"
                     /locus_tag="JN331_003170"
     CDS             7323..8495
                     /gene="iadA"
                     /locus_tag="JN331_003170"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7261245.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            8560..9471
                     /gene="hypT"
                     /locus_tag="JN331_003171"
                     /gene_synonym="qseD"
     CDS             8560..9471
                     /gene="hypT"
                     /locus_tag="JN331_003171"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY7261246.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(9464..9856)
                     /gene="iraD"
                     /locus_tag="JN331_003172"
     CDS             complement(9464..9856)
                     /gene="iraD"
                     /locus_tag="JN331_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY7261247.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(9853..9936)
                     /locus_tag="JN331_003173"
     CDS             complement(9853..9936)
                     /locus_tag="JN331_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY7261248.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         2659 a         2561 c         2486 g         2382 t
ORIGIN      
        1 gcattatatc tccgtgctag aaaaaacaag tttaattctg ttttttcacg gaaggtcact
       61 atggcttcga caaatgcgca tcaaataaaa aataatgatc aaaactcttt atgtggtctg
      121 ggtgacaaaa tacgccgcct gaccgccgga gtttgtttat ttacacaaat ttttttcccc
      181 gtcatggcga cggcacaaaa tgtggtacat gctaaacctc agacgacagt ttcatctgcc
      241 ccccctctac tagaaaataa tacggtgcct tacacgcttg gtgcgctgga atcagcgcaa
      301 agcgttgctg atcgcttcgg tatttcactg gaggagcttc gtcgtcttaa tcagttccgt
      361 acttttgctc gcggctttga taacgtgcgc cagggtgaag aactggatgt tccggcaaca
      421 acctcgcaga aaagccatga gcaacaaaat gccgtaccgc ctgcgaatgg cgaaaacacg
      481 ctggagaatc aaatagccag cacctcgcag cgagttggca ctctgctttc acaagatatg
      541 aatagtgagc aggccagcgg catggcgcgt ggttgggcgt cttcagaagc ctcaggcgcg
      601 atgactgatt ggttaaataa ctttggtact gcgagaatct ctctgggtgt ggatgaagat
      661 tttagcctga aaaattcgca attcgacttc ctgcatccgt ggtatgacac acccgattat
      721 ctgctcttca gccagcatac ccttcaccga acagacgatc gtacccagat caacaccagt
      781 ttgggctggc gtcatttcac ctccagctgg atgtcaggca tcaacctgtt ttttgaccac
      841 gacctgagcc gctatcactc ccgcgcgggg cttggcgcag aatactggcg tgattatctg
      901 aagttgagca gcaacgctta tattggcctg accggctggc gtagcgcacc agaattggat
      961 tatgactacg aagcccgccc ggccaacggc tgggatttac gcgcggaagg ctggttacct
     1021 gtctggccac aactgggggg aaaactggtc tatgaacaat actatggcga tgaagtggcg
     1081 ctgtttgaca agaatgatcg tcaaagtaac ccccatgcta ttaccgcagg cctcaactat
     1141 acccccttcc cgcttctgac tctcagtgcg gaacagcgtc aggggaagca aggtgaaaat
     1201 gacacacgtt ttgccgttga tctgacctgg caacccagca gttcaatgca gaaacagctt
     1261 aatccggacg aagtggccgg acggcgcagt ctggccggta gtcgttatga cctgattgat
     1321 cgcaacaaca acatcgttct ggaataccgc aagaaagagc tgattcgcct gagtctgctg
     1381 gatccagtga aagggaagtc tggagaaata aaaccgctgg tttcctcgct acagaccaaa
     1441 tatgccctga aaggctataa catcgaagcc gctgcgctga aagctgccgg aggtaaagtc
     1501 agcacgtctg gaaaagatat cacggtcacg ctgccaggtt accgcttcac taacacccca
     1561 gaaaccgata atacatggtc gatagacgtt accgccgagg atgtaaaagg taacctgtca
     1621 cggcatgaac aaagcatggt agttattcag gctccgacat taagccagaa agattctctg
     1681 ttatccgtca atccgctaac cgtggctgca gataaaaaat cgacgaccac attgaccgtt
     1741 actgcgcacg attccgacgg aactccggtg ccggggctgg cgctgcaaac ccgcagtgaa
     1801 ggcgttcagg atatcaccct gtctgactgg acagataacg gtgatggtag ttacatacag
     1861 atgctgaccg ccggaacgac atcaggttca gtaacactga cgccgcaaat taacggtgag
     1921 agtgcggtaa aagaatccat cgtcgttaat atcgtccctg ttgtctcatc ccgcgaccat
     1981 tcatcaataa caattgataa cgtatcgtat tatgccggag acgacatcaa ggttagggtg
     2041 gaactgaaag acgacagcaa tcaaccggtt gcatatcaaa aagaggaatt ggtaaaagcc
     2101 gttactgtcg aaaacagcaa acctggcgcc acgattgtct ggcacgaaga gcagccgggg
     2161 gtttatgccg cgaattatcc ggcccataag caagggactg cactaagggc acaacttagc
     2221 cttcacaact ggaatgctcc actgcaatca catatttata acattgaggc aaaccagaat
     2281 aaggctcgcg tcgccacatt atcagcgacg aataatgacg tttacgctga taaaaagaca
     2341 tttaataccc tcactatcaa cgtcactgat gagagtgata atcccctgac aaatcatcag
     2401 gtcaccttta agaatgaaaa aggaagcgcg gaatttgtcg aaccgccgca gcaaaatacg
     2461 gatggatacg gtgttgccac aatcaacatg gtaagccagg ttgcggaaga aaacacgatt
     2521 agcgccacgc tgccaaatgg tttctcacaa cggataattg cgaaattcgt tagcgattcg
     2581 agtacgccaa aattcaaaca actggttgcc gatccagata ccattattgc tggcaacagc
     2641 cagggcagta ctctgaccgc catcgtcaca gactttcata acaacccgtt aaaagatatg
     2701 aaagtgaatt ttgtggcacc tggtggctcg caactggaca acacgaccgc cacaacagac
     2761 cagtccggta ttgtgcgggt gcacctgacc agttcaaaag ctggtagcta ttccgtcgat
     2821 gcctcgcttg aggtggataa aaatattcac cagtcggtca cgatcaccgt ggtcccaaac
     2881 agggaacaat cggtaatgac cttgaatgcc gggtcgggca gtgcgatcgc taacaataca
     2941 aatatcgtta ccctgactgc cagtgtgaaa gatgtttatg gacacccgtt gccggatgag
     3001 gatgtgaaat ttaccttgcc agcctccatg accgggaacc tcacgctaag tagtgaaacc
     3061 gcccgcaccg atgcaaacgg tgatgccgtg gtcacattgc gaggcacaaa agcgggtgag
     3121 tttacagtta cggcgacgct gaccagaaat aataccgttg cttatcagca agtcactttt
     3181 attggggata caaacagtgc gcagctccag ccgctgactg cctcattaaa ttccattgtt
     3241 gcgggtaaca gtacggggag taccctgacg gcaacgatcc tggacgctta ccaaaatccg
     3301 cttaaagacc agttggtcac tttccagagt aacgatgtca ctctaagcga aacagaagtc
     3361 accaccaata cgctgggtca ggcgacggta acaatgacca gcaatattgc cggacaacat
     3421 aacgtcgtgg tgagccggaa agcgcaagct tccgataata aaacgtttag tttatcagtg
     3481 ctgccggatg aaagttcggc gaaggtaata agtataaccg gagccgaaaa aacgataacg
     3541 gtgggcaaaa acatcacgct acggatactc gtccaggacg cgtttaacaa tgtaatcgcg
     3601 ggtcaacgcg tcagattaag tgcgcagcca acaactaaca ttacgatagg cgatacggct
     3661 tacaccgata ataacggtta tgcgtacgtt aaccttctca gcacccaacc tggggtttat
     3721 caggtgacgg caacgctgga caataacagt agtagtaagg ttgacgtgaa tgtggcaaat
     3781 ggcaaactcg agttaacatc atcgaaacca gaaaccacgg tccataatag tgagggtatt
     3841 acgctgaccg caacggcgag aaatgcgcgg ggtgaattga tgccagggca aattatcacc
     3901 tttagcgtaa cgcctgaagg tgcaacgcta agcaatacag gggaagtcct tactgaccag
     3961 tcaggtcagg ccaaagtgac gctgaccagt gacaaagtga atgtctatac cgttacggcc
     4021 ataatgggca aagatgttcc cgttcagagc caggtaacgg ttgcggttaa ggcagatgct
     4081 aaaacggcac atgttgtgag cgtcgtggct tctcctgaca ccatcaccgc cgacggcatc
     4141 gatagcagca ccatcacttc acgagtagaa gatgattacg gattcccggt tgaaggtgtc
     4201 gatgttagtc atggcttaga caccaaaggc agcccggtag ttaatattcc aactacgcgt
     4261 accgatcagt ccgggcaagt cacggcgaca ataaccaata cattggcaga aaccttaaca
     4321 gtcaatgtgc aagttcctgg tacagccaac caatccgcaa ccattacatt ggttgccggc
     4381 acggccgatg aaagtaagtc aattttgaaa tccgatgttg acactctgaa ggctgactac
     4441 cagcagagcg caaaacttac gctaacattg caagacaagt acggtaaccc gatagtgacg
     4501 tctgatcatc tggaatttgt ccagtcaggc cccttcgtga actttctcaa gttgagcgat
     4561 attgattaca gccaaagaaa ttatggcgag tacaccgtga ctgtcactgg cggaaaagag
     4621 ggaacagcga ccctcattcc catgctgaac ggggttcatc aggcaaactt aagcgtatcg
     4681 ctgaatctca tccactcgat aaaagaaatg tccggtcatg tcactgcaaa caaccatacc
     4741 ttctccacgg ctaaattccc gagcgaaggc tttgcagggg cgtattacac actcaacaat
     4801 gataactttg aagcgggtaa aaccgttgat gattatatgt tttcaagttc acagagttgg
     4861 gtgtctgtcg atgcttcagg taaggtttct ttcgcaaata tcggcgatca aacgtcagtc
     4921 acaataagcg ctgttccccg acaaggaggt acaacctacc agaccttaat taagctgaaa
     4981 ggctggtggg tgaataatgg aaatcatacc aatatctggc tagctgccaa tgcgctctgt
     5041 catgctaaaa atgatggata taatcttcct agcatcacac atttgacgtc tggcgaaaac
     5101 aaacgcacgc agggatcgct gtatggtgaa tgggggaacg ttggagcgtt ttccagtaat
     5161 tcgcaattta caccgggagc ttactggaca agtgaatctg atgattacag tcggcactac
     5221 tatgtgcaga tgctaaccgg tatgaccgga agcgacgctg attccagccc ccaactgacc
     5281 gcctgccgta aatcacttta atttcagcag ttgttattaa aggataccct tttacgaggg
     5341 tatcccttca gactgataac aaacgctaca ttgccggatg tgctgcgctt accaggccta
     5401 tataacccct gcaatatatt gaatttattg tttttgtacc ccagtcggca taaacgacca
     5461 ctcaagaaac tcaacagcaa ccgttgatgt cagccagacc ccgttttccg cttgccagaa
     5521 agggatgcct cgtttcgcca tttcctgtgc tttgacggtt aagacaaccg gcgatccgtg
     5581 tcttgctccc actttgcgtg ccgttgcttc atcagcagag agatgcacgt aatgacgatc
     5641 ccctgcaatc agcccctgtt tttttatctc gtcaaggaaa cggcttgccg taccgtggta
     5701 gagaaactgt ggcggtgttt tttcggcaaa ggaaatagcg acctgcgaag tcgaatgccc
     5761 ctgaaccgcg cggatgcacc taccatcact ggaataacta aaacgctttt tatcgctggt
     5821 tgcgaccacg gtatccagca atgcacgggt taatcgcttg cccgcttttt gggcacaaag
     5881 tatgagctta ttgatatccg cccatccttc acggtccagt acgataccaa tcgcctccgg
     5941 tttgtgtcgg aggacaaaac ttaaaaactt gctcgtatcc gccagctctt tttcgttgta
     6001 ctttgccatc gcgttttcgt ccctggattt tctgttttta tttactaatg tcattgtcac
     6061 tgggtttatg aattttctgc aactcaatcc tgcgcagaac cttttccaca cagcgtaatt
     6121 tttccctatg caaaaaacgc atagccagaa acatacttgc attttcccca tacccacatg
     6181 gctgataagg tttttcagtt ccaggaattt tcctggctca agcaacacac aaacacaaaa
     6241 aaacaatggg gattgttatg acacaacaag gggatgctgt agccggggag ctggcgacag
     6301 aaaaagtggg cattaaaggg tatctggctt tttttctcac cataatcttt ttttccggtg
     6361 ttttttccgg aacagacagc tggtggcgcg ggggctaaag atggctttct ctttgcgctg
     6421 gagctggcac cgtcagtcat tctctctctg gggattattt caattaccga tggtcttggt
     6481 ggattacgcg ccgcgcagca gttaatgaca cctgtgctaa aaccgttgtt gggcattcca
     6541 gggatctgct cgctggcact gatcgctaac ctgcaaaaca cggatgccgc cgccggtatg
     6601 accaaagagc tggcgcagga aggtgaaatt accgaacgcg ataaagtgat tttcgccgca
     6661 tatcaaacca gcggtagcgc catcatcacc aactacttct cttccggagt cgcggtgttt
     6721 gcctttctgg gcacgtccgt aatcgtacct ttagccgtca tcctggtgtt taaatttgtc
     6781 ggagccaaca ttttgcgcgt ctggcttaac ttcgaagaac gtcgcaaccc aacgcaagga
     6841 gcgcaagcat gacaactcag gtccgcaaaa acgtcatgga tatgtttatc gatggcgcgc
     6901 gccgtggctt tactatcgcc acgaccaacc tgctgccaaa cgtggtgatg gcgtttgtca
     6961 tcattcaggc gctgaaaatt accggcctgc tcgactgggt ggggcatatc tgtgaacccg
     7021 taatggcgct gtggggatta cccggtgaag cggcaaccgt ccttttagcc gcattaatga
     7081 gtatgggcgg cgcagtcggt gttgccgcca gtctggcaac cgctgacgca ttaaccggcc
     7141 acgatgtcac cgttttactg cccgcaatgt atttaatggg caacccggtg caaaatgtcg
     7201 gtcgctgcct ggggacagcc gaagtgaatg ccaaatatta cccgcatatc attacggtct
     7261 gcgtgattaa cgcattgctg tcgatctggg ttatgcagct tattgtttaa caaggagtta
     7321 ccatgattga ttataccgca gccggtttta ccctgctgca gggagcgcat ttgtatgcgc
     7381 cggaagatcg gggaatttgc gatgtcctcg tcgctaacgg caaaattatc gccgttgcca
     7441 gcaatatccc ttctgacatt gtaccgaact gcacggttgt cgatctcagc gggcagatcc
     7501 tctgcccagg ttttattgat caacacgtcc atttgattgg cggtggcggc gaagcaggtc
     7561 ccacgacgcg cacgccggaa gtggcgctaa gtcgcctgac ggaagcgggc gtcacgtcag
     7621 tggttggtct gctgggcacc gactctatct ctcgccaccc ggaatccctg ctcgccaaga
     7681 cccgtgcgct caatgaagaa ggcatcagcg cctggatgct gaccggcgct tatcatgtcc
     7741 cttcccgcac tattacgggt tccgtggaaa aagacgtggc gattatcgat cgtgtgattg
     7801 gcgtgaaatg cgccatctct gatcaccgtt ctgccgcacc ggacgtttat cacctggcta
     7861 atatggcggc agaatcccgc gttggcggtt tgctcggcgg taaacctggc gtcaccgtgt
     7921 tccacatggg cgacagtaaa aaggcgttaa agcctattta tgacctgctg gaaaactgcg
     7981 atgtgccgat cagcaagctg ctgccgaccc acgttaaccg caacgtaccg ttgtttgagc
     8041 aggcactgga gttcgcgcgc aaaggcggca ccatcgatat caccagcagc attgacgaac
     8101 cggtcgcccc tgccgaaggt attgcccgcg ccgttcaggc gggtattccg ctggcacgcg
     8161 tcaccctcag ctccgacggc aacggtagcc agccgttctt cgatgacgaa gggaatttaa
     8221 cccatatcgg tgttgcaggt tttgaaacgt tgctggaaac cgtgcaggtg ctggtcaaag
     8281 actatgattt cagtatcagc gatgccctgc gcccgctcac cagtagcgta gccggtttcc
     8341 ttaacatgag cgggaaaggc gaaattctgc caggcaatga cgcagactta ctggtcatga
     8401 cgccagaatt gcgcattgag caggtatacg ctcgcggcaa actgatggtc aaagacggca
     8461 aagcctgcgt gaaaggaacg tttgaaacgg cttaaacggt tgtcagccgc cctaatggac
     8521 gtagaatgcc cccaagggcg gctgacagag taaaacgtaa tggatgactg tggtgcggtt
     8581 ttgcataata ttgaaaccaa atggctttat gattttctga ctctggaaaa atgccgcaat
     8641 ttttcccagg cggcagtcag tcgcaacgtc tcgcaaccgg cattcagccg ccgcatccgt
     8701 gcactggaac aggcgattgg cgttgaattg tttaaccgcc aggtgacgcc actgcaactc
     8761 tcagaacaag ggaaaatctt ccattcgcag atccgccatc tgttgcaaca gttagaaagc
     8821 aaccttgcag agctgcgtgg cggcagcgat tacgcgcaac gtaaaatcaa gatagccgct
     8881 gcacactctc tttccctcgg tctgttaccg tccattatca gccagatgcc gccgctcttt
     8941 acctgggcaa ttgaagccat tgatgtcgat gaagcggtcg ataaactgcg tgaagggcaa
     9001 agtgactgta ttttttcctt tcacgacgaa gatttgctgg aagcgccgtt tgaccacatt
     9061 cgcttatttg aatctcaatt gttccctgtc tgcgccagtg acgaacacgg agaagcactt
     9121 tttaacctcg cgcagccaca ctttccgtta ctgaattaca gccgcaactc ctacatgggg
     9181 cgattgatta atcgcaccct gacgcgccac agtgagttaa gtttcagcac cttttttgtc
     9241 tcttcgatga gcgagctttt aaagcaggtt gccctcgacg gctgtgggat tgcctggctg
     9301 ccggagtatg ccatccaaca agaaattcgc agcgggcagc tcgttgtgct gaatcgggac
     9361 gaacaggtga tcccgattca ggcctacgca taccggatga atacccgcat gaatccagtt
     9421 gccgaacgct tctggcgtga actgcgcgag ctggagattg tgcttagctg acattctcaa
     9481 gcgtcgcact gcgccaaccg ccattatccc agaccagatc aaacgttagc ggttcatcgt
     9541 cgtggtacag cattatctca aggctgatcg ccagagtgtg gcagtacgct tctttcaaat
     9601 taacctgcaa cgccgcaatc cgtggctcat gaaataacag tgcagaacgc agctgacggc
     9661 aataccagct ctggagatgt ttgtcgctcg ggttatgcac ttgccactcg ggcatgcctg
     9721 aaacaagtaa tgacggtaat gccgaatgag gagaattaaa aagagtgagg agatcgctgc
     9781 ataccgattt acgcagcaat gacgttttat tgcctgaaat ttcaggtaaa acagcctgaa
     9841 gtgattggcg catcattttg cgcactcctg acgtttagca acatatgcgg cttgccctga
     9901 acaccgggca ccactgtatt gatgctcatt ttccatagcc attccctttt gttcattaca
     9961 tccatgtcag tctgagacag gcctgaatcc tgtcagcaaa gtataaaacc ttacttgcct
    10021 atgaatatct actgactttt caggaaaaaa gtcatgattt gataaaaatc atcaaactca
    10081 aaaaaaaa
//
LOCUS       DADLSB010000134        10075 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.134, whole genome shotgun sequence.
ACCESSION   DADLSB010000134 DADLSB010000000
VERSION     DADLSB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.134"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..380
                     /gene="fliR"
                     /locus_tag="JN331_003174"
     CDS             <1..380
                     /gene="fliR"
                     /locus_tag="JN331_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="FliR, with proteins FliP and FliQ, forms the core
                     of the central channel in the flagella export apparatus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAY7261249.1"
                     /translation="FLTFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIF
                     LNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAP
                     FCKHLFSEIFNLLADIISELPLI"
     gene            670..1293
                     /gene="rcsA"
                     /locus_tag="JN331_003175"
     CDS             670..1293
                     /gene="rcsA"
                     /locus_tag="JN331_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY7261250.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(1337..1579)
                     /gene="dsrB"
                     /locus_tag="JN331_003176"
     CDS             complement(1337..1579)
                     /gene="dsrB"
                     /locus_tag="JN331_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY7261251.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            1688..1915
                     /gene="yodD"
                     /locus_tag="JN331_003177"
     CDS             1688..1915
                     /gene="yodD"
                     /locus_tag="JN331_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY7261252.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            2213..3028
                     /locus_tag="JN331_003178"
                     /pseudo
     CDS             2213..3028
                     /locus_tag="JN331_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(3025..4719)
                     /gene="yedQ"
                     /locus_tag="JN331_003179"
     CDS             complement(3025..4719)
                     /gene="yedQ"
                     /locus_tag="JN331_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAY7261253.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGICMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(4890..5072)
                     /locus_tag="JN331_003180"
     CDS             complement(4890..5072)
                     /locus_tag="JN331_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY7261254.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(5151..6068)
                     /locus_tag="JN331_003181"
     CDS             complement(5151..6068)
                     /locus_tag="JN331_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY7261255.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6241..7161
                     /gene="yedA"
                     /locus_tag="JN331_003182"
     CDS             6241..7161
                     /gene="yedA"
                     /locus_tag="JN331_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY7261256.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(7150..7620)
                     /locus_tag="JN331_003183"
     CDS             complement(7150..7620)
                     /locus_tag="JN331_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY7261257.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(7601..9019)
                     /locus_tag="JN331_003184"
     CDS             complement(7601..9019)
                     /locus_tag="JN331_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY7261258.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(9086..9781)
                     /locus_tag="JN331_003185"
     CDS             complement(9086..9781)
                     /locus_tag="JN331_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAY7261259.1"
                     /translation="MELQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(9821..10075)
                     /locus_tag="JN331_003186"
     CDS             complement(9821..10075)
                     /locus_tag="JN331_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261260.1"
                     /translation="MRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGS
                     TAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         2473 a         2652 c         2409 g         2541 t
ORIGIN      
        1 tgttcctgac atttaacggt catttatggt tgatttcact gctggtcgat acctttcaca
       61 ccctgccgat tggtagcgaa ccgttgaaca gcaatgcgtt tctggcaccc accaaagcag
      121 ggagtttgat tttccttaac gggctgatgc tggcgttacc gctcattact ctgttgctga
      181 ccctgaatct ggcattaggt ttactgaatc gtatggcccc gcaattatcc atttttgtta
      241 ttggatttcc attaactctg actgtcggca tctctttaat ggcggcatta atgccgttaa
      301 ttgcgccttt ttgcaaacat ttattcagtg aaatttttaa tttgctggct gatattatta
      361 gtgaattacc attaatataa ttccgtaacg tttatcatgc taccctaagg attatccgaa
      421 aaataatatc tgcgaacatc ttccaggata ctcctgtagc gaaatatttg ttttaagctc
      481 actcacatat cgcaacattt actttacttt aagacaattc caggcaaatt atacaacact
      541 ttacgggata gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt gacccatgtt
      601 gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg cattgagtga
      661 gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact aggtttaacc
      721 gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga aaccgttgat
      781 gaccttgcca tagcttgtga ttcacagcgc ccttcagtgg tgtttattaa tgaggactgt
      841 ttcatccacg atgcttctaa cagtcagcgc atcaagcaca tcattaatca acatcccaat
      901 acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata tctattggtc
      961 agaaaaaatt tattgatcag ctctaaatcg attaaaccgg aatctctcga cgatatcctt
     1021 ggcgatattc tgaaaaaaga gacaacgata acctcgtttt taaatatgcc gacgttatca
     1081 ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg caggtcaggg aaccattcaa
     1141 atctccgacc aaatgaatat caaagccaag accgtttcat cgcataaagg taatattaaa
     1201 cgtaagatca aaacgcataa taaacaggtt atctaccatg tcgtccgact gacggataat
     1261 gtgactaatg gtatttttgt caacatgcgc taacacattc tgactggtgg tttccctcca
     1321 gtcaggctga ataagattac tctgctttct caacaaagat accgtcctga tgccctgctt
     1381 cattaaagaa ccagatgccg agcgggtagt cttccagcga aaccaggtac attgtgcctt
     1441 cactaaactc ctcaactgcc agtaccactc caggacgacg cggaccgcca tccgttttga
     1501 ctgttacccg atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc agtgtagaac
     1561 aatttcgttt ttctggcagc gccaggcgcg cgcgagtgct gattttctcg acggtctata
     1621 cttaagagat gccaggcgga cttaacgact ggcggcaaca acagagtaac ggttgcgagg
     1681 aaagatgatg aaaaccgcaa aagtgtacag cgataccgca aaacgtgagg tcagcgtcga
     1741 tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa agcgaagttc atcgcagcca
     1801 gaacgattct gaacacgtta gcgtcgatgg acgtgaatat catacatggc gtgaattggc
     1861 ggatgccttc gaactggata ttcatgactt cagcgtctct gaagtgaatc gttgaatgca
     1921 caataaaaaa atcccgaccc tgagggtgtc gggatgaaac ttgcttaagc aagaagcact
     1981 taaaaaattc gttacaccag gaaatctgat gtgttcatca ccttatctgc aatttttttc
     2041 gctgacaaga aaatattcgc gctatgaatg attaacgcta cttatgaata gaaatgtgac
     2101 gcgcgtcacc ccttggtggc tctggtgtca acgggatcgc tcccgaaaaa atcatctttg
     2161 attatttgtg ctgttttgag aatcatcctg gtaaggggag tactgcgcga ccatgttttc
     2221 aattcaacaa ccactactgg tttttagcga tcttgatggc accctgctgg acagtcatag
     2281 ttatgactgg caaccggcag ccccctggct cagccgttta cacgaagcca atattcccgt
     2341 cattctctgt agcagtaaaa cttcagcgga aatgctgtac ttgcaaaaaa tgttggggct
     2401 acaaggctta ccgctgattg cagagaatgg cgcagtgatc cagcttgctg aacaatggca
     2461 ggatatagac ggttttccac gcatcatctc aggtattagc catggcgaaa tctgccaggt
     2521 tttaaatacg ctacgcgaga aagaacattt taaattcacg acttttgatg atgtcgacga
     2581 tgcaaccatt gccgaatgga cgggattaag ccgtagctag gcggcgctga cgcagctgca
     2641 tgaggcgtcg gtaacgctaa tctggcgcga cagtgacgag cgtatggcac aatttatcgc
     2701 tcgtctgaac gaactgggct tgcagtttat gcagggtgcg cgcttctggc acgtactgga
     2761 tgcctctgcc ggaaaagatc aggctgccaa ctggattatc gcgacctatc aacaattgtc
     2821 aggcagacgc ccaaccacac ttggcctggg cgatgggcca aacgatgcgc ctttactgga
     2881 ggtaatggat tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtac atctgcatga
     2941 tgaggatccg gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggct ggcgtgaagg
     3001 gctggaccat tttttctccg cccgttaagc gttatcgctc gcgaataccc gattgcgccc
     3061 ggcctgtttt gcgaggtaaa gccgacggtc ggccaatgac tggagttgtt caaaatcata
     3121 atcaccggtt tcctcgctgc tacttacccc cagcgaggca ctgatgcgta tcgttgtact
     3181 cttggcgatc agcatctctt tttcatttaa cttcaggcga atacgttctg cgacctccgc
     3241 agcctgcgtc agattcgcgc ctggcagaat cacacaaaac tcctcaccac cgacccgccc
     3301 ggcaacgtcc tgcgcacgca aggaactgct aattaatccg gcagcatgag aaagaacacg
     3361 gtcgcccgcc tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa gatcgacctg
     3421 aatgacagaa aaaggatgtt ggtgcgtctg acacaattta gcgagcggac gggctttttc
     3481 gaacagtgcg ccacggttat ataaacgcgt taaggtgtcg tgccacgcct gccactgcaa
     3541 cgagctttgc agaacataca tgttgctgac catccggcga atcacatacc aggagaggag
     3601 taacatggtg gtaaagagcg cccacagcag ggttaatgca atgctgatac tgccgaaatc
     3661 gccgcgcacg ccttcgctta gcgtatggac gcgcaccagc acaccgtcga aatggtccag
     3721 acgttcccag ctaacatagc gactgtccat acaaatgccg ccccgcgtgt catgttccat
     3781 tgcctgcgcc agcaaggcca gttcacgagg atcaaaaata ttccctgttg gatggtcagg
     3841 attggaagag gtcaaaaatc tcagcttact gtcatagagc tgatactcac catcgaggtt
     3901 tttatcgatg gcgtttctta aaaattgctg catagtacgc acgggaatac tcatccccag
     3961 cacgccatac cagtagttat tactgtctac cggaacactg acggtaacct gcggttcagt
     4021 attgctggcg tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac gattttctcg
     4081 ttgcgaatgg ccgataaacc agggttgggt gacatagccg taataacgcg ttggtacatt
     4141 gcgcgtaaac aaggtcggct gcgtcgaaac gtaaaatcca gcacgcgaga catacatcgc
     4201 ctgttcaacc atcgacgagg agttgtgcgc cagtcgcagc aagtaaccaa cttccagtgc
     4261 agcggtaatt tcattgtcga ggctttcatt ttcgcgagac aggaaattcc cctcgctgac
     4321 taatgcatcc gatacgccat tgactggcaa ggtgcgccgt cggttgagtt cgatttgcca
     4381 ggcgtgctcg tcgcgatgct gttcgaactc ggttacggca ttacgcagag aggtgaaatc
     4441 cagtggcgcc acgagagctt cgcgcatgcc attacgcaaa aagatcagtt tgtcgacgtt
     4501 atactgcaaa tgcttatcga gcgcgttggc tacgttttcc agatgattac gctggctgga
     4561 gatataggca tcttccagca ccaccacttc acgccaggtg agcaaggtgg aaaaaagcaa
     4621 taccacgata aagcagagat taacgacatg accgggcccc aggcggcgtg cgagtttttt
     4681 caaccagctc tggttttcca tttttgtctc gtgctgcacc ctgacgactc ccaacctgct
     4741 tttttatgat tctggcataa cccggcgata gcgtaccgca aaacagcgat aattttataa
     4801 tggtggcatg ataagacgcg actatcattc attgcaggca ttgggacacc aggccggatg
     4861 tgatccggcc tggtgttaat gatacccttt caaacttctt ccgcagaacg actcccctcc
     4921 cccggaacaa gatcggtttc atgaaaagct tcccgcttga ccccgtaacc gtcataccaa
     4981 cgacactcaa ccataccgct ggagtatcca gtgacaatca tccgcgggcc gccctcttta
     5041 accgtaactt cctcactaac cataaagctc atactcgcct ccttttttcg agtgaaactt
     5101 gttcacctta gttgaagatg gcgaattttg cataatagcc agtgcgagta ttaatgtgcc
     5161 tgaccgcgca ttttcgctac tgctttcact cccagcacta cgatgccgcc gatgataaat
     5221 ccaagaatca gatttaaaac agtcggtaat atcatcgcca ccactgcact ttgctgcccg
     5281 gcgatatgtt caatagcgtg atgcagcggc gcaataccat gtaccacaat cccgccgccg
     5341 acgaggaaca tcgccagcgt gccgacaatc gataatgctt tcatcagcca aggcgcgata
     5401 atcaataacc ctttacctaa tgcctgcatc agcgcgctgg atttttccgc cagccaatac
     5461 cccaggtcat caatcttaac gataacccct accagaccgt agacgcccac ggtcactacc
     5521 agcgcgatgc ctgaaagcac cagcacctga ttaagcaacg gcgcctctgc cacaatcccc
     5581 agcgtgatgg cgacgatttc cgcagacaaa ataaaatcgg tacgaatcgc ccctttaatt
     5641 ttgtcctttt caaacttcag cggatcctgc gccgccagct tctccagacg ctgctggctc
     5701 tgcgctggat cttctttatg tttacgcgcc tccagcatat gcagcacttt ctctacccct
     5761 tcaaagcaga gaaacgcgcc accaatcatc aacagtggcg taatcgccca cgggataaac
     5821 gcactgatga tcagcgccag cggcaccaga atcactttat tgatcagcga tcctttcgcc
     5881 acgccccaga ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg ttgcgcattg
     5941 agcgataaat catcccctaa tacaccggcg gttttcttcg ccgccagttt gcccatcaca
     6001 gagatatcgt ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact actcccggcc
     6061 aaaagcattc cctctcattt ttattgttaa agggtgtaag tatgaagcaa aaacggcagc
     6121 tctgatactt gcttcacagg tcaacattta tttaacaatc aagtgcaatt atatctctcg
     6181 ccagcagaat gattttcacg tttactatga accacctttt tatttccgcc gtgagggatt
     6241 atgcgtttcc gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta tatcatttgg
     6301 ggctcaacct attttgtcat tcggattggc gtgggaagct ggcctccgtt aatgatggcg
     6361 ggcgttcgat tcctggcagc cggtatttta ttgctggcat ttttgctact gcgcggacac
     6421 aaacttcccc cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt attgctggct
     6481 gtcggtaatg gcatggtgac ggttgccgaa catcaaaatg ttccttccgg catcgccgcc
     6541 gtagtgattg caaccgtgcc cctctttacc ctgtgcttca gtcgcctgtt tggcattaaa
     6601 acgcgcaaac tggaatgggt gggtattgcc attgggcttg ccggaatcat catgctcaat
     6661 agcggtggaa atttaagcgg caatccgtgg ggcgcgattc tgattttaat cggctcgatt
     6721 agctgggcgt ttggctcagt ttatggctcg cgtattacct tacctgtagg gatgatggcg
     6781 ggtgcgattg agatgctggc ggcaggcgtg gtgttaatga tcgcatcgat gattgcgggt
     6841 gaaaaactga cggcgctccc ttccctgtca ggcttccttg cggtcggcta tctggcgctg
     6901 tttggttcga ttatcgccat taacgcttat atgtatttaa tccgtaatgt cagtccggct
     6961 ctcgccacca gctacgccta cgttaacccg gtggtcgcgg tcttgctggg tacgggactg
     7021 ggtggcgaaa cgctgtcgaa gattgaatgg ctggcgctcg gcgtaattgt tttcgcggtg
     7081 gtactggtca cgttgggaaa atatctcttc ccggccaaac ctgttgtcgc gccagttatt
     7141 caggatgcat caagcgagta aatgaatccc ctgcgtgtcg atctgcgcgc tggcaccttc
     7201 gccgcagatc cactcttcca gacgctcggt aagcgccgca tccgtcagct tctcgcgcct
     7261 acgtaacgca cactcccaga caatcagtac gcgccagccg agctcctgca agcgactgat
     7321 atcgcggcga tcgcgttcaa catttttacc tatcttctcc agccagaact cagttcgagt
     7381 cgcaggcact ttaaacagat agcagtgatg atgatgccag aagcagccgt gggtaaatat
     7441 cacgcagcga tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg cgtcctgaac
     7501 gcgaaacgca aggccctgcc cggttaacaa actggcgagg cgcttctcaa tcgccgtatc
     7561 acgcgtggca atcgcgcgca tatttttgct gcgggtggcc ttatcgtgaa cgtcgaccat
     7621 gttgtgcctc ttgctgacgc aacgccaccg cctgtttgat ttttggctca agcagttttg
     7681 ccaccgcggc aaagaccggc acgaccaccg agttgccgaa ctggcgatag gcctgagtgt
     7741 ccgaaaccgg aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt aagcgcgcgc
     7801 attcacgagg cgttaaccga cgtggacgat gttgctgatt cagcggatcg tcaaagtctt
     7861 tctcacccgt ggccatatcc caaccgcgat cgattaaaat ttccgcgcca tctttgtaat
     7921 aacgagcaga cagcgtacgg gtgacgcttt gcggattgtt cggataaacc attccataac
     7981 cgaagccgtt accgcgcgcc tgatgttttt tcgcatatcg atagagatac ttccacagca
     8041 ccggcgtcag gatatatttc gcctcaacca tcgggtccag cagctgcgcc agcgtcactc
     8101 gctgtgcagg gaaacattcg ctgatatcac gcagagtaaa atcggctttc agattcagat
     8161 cgcgacgaaa acccaccagc acgatgcgtt cacggtgctg cggcaggaaa tgtttaccat
     8221 cgatgatttt cggatcgtcc ggcccgttat cttctgcatc ggcaacgtca tagcccagtt
     8281 cgtccagcgt ctgcatgatg atgcggaacg tttttccctt atcgtgactc ttcaggtttt
     8341 tgacgttttc gagcacaaac atcgccggac gacgcgcgtc gataatgcgc accacatcaa
     8401 agaacagcgt gccctgggta tcgcaggcaa aaccgtgcgc ccgcccgagc gagttctttt
     8461 tcgatacgcc agccagcgaa aatggctgac aagggaaacc ggccagtaaa acatcgtgtt
     8521 caggaatgtg ttgacgaata tgttccgccg ccgcctcatc actcacgcct tcttgatgac
     8581 tgagggtgat gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg caataatggt
     8641 tggctttata agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg cactgtccac
     8701 caatcgattc aaaaccacga cggatgccgc caattccggc aaatagatcg ataaagcgaa
     8761 acgcataatg cggatgatgt tccggtggtt tgggtaacaa cgtttgcaga tgggcaaact
     8821 ctttctcact taaacggtgc catgccgagt cattcgccag cgcacgtttt aaaattgccg
     8881 cgctccagtg attctcaccc acaccattaa gctgcgccac cagcatttta acatcataaa
     8941 tttgcagcag tttctccagc attgcctgtg cggcattccc ggtgctgtat gaatcggtta
     9001 ctgatatatt ttcctgcata gatttaaccg gccatctgag atggccgaca ggttaacata
     9061 attactgccg tttagggcca cgcgctcagc cagcgggtga aaacgcatct atcaccttgt
     9121 gatcgatacc ttcattctcc cccgccagtt cggcaccgag cttcgccata aactcgacta
     9181 aaaattgcgc gttgtgctgt gccagctgct tgcctcttgc ggtttgcatg gtttgcggta
     9241 atttcagtaa ctttgtctgg aaatgatcca gcgcgtagcg tttatcatca agcgggcgat
     9301 gctgtgcgaa cggatcttcg ccatcaaaca gcgccaagcc caatgcccct gaaacggcaa
     9361 atacgcgcgc caggccaatc gcccccaggg cttccagccg atccgcatcc tggacgattt
     9421 tagcttccgt tgttaagggg gcgatttgcg cgctgaaact gtgagcggca atggcatgac
     9481 aaacggcctc aattttctcc gccggaaatt gcacgaactc ttcacggagc aggcgacgcg
     9541 tctcttctgc cgccaggatt gaagaacgct gccgttgcgg atgatttttc gccaggctca
     9601 cgatatcgtg aaaataacag gcggttaaaa tcaccagcat atcgacatcg tcatctgccg
     9661 ccagtttctg tgccgtagcc cagacacggc gaaaatgaca caggtcatgc gccgcgtcct
     9721 gatgttgatg atggtttttc aaccagttct cgaactgtgc ctgccagtgt tgtaactcca
     9781 tacgcgctcc gttgccaaag agtcgcaacc ttagcagttt ttattcatag cgtctgcaac
     9841 gtgcgggacg aggtcgggtg taccatgcaa tgccgcctaa caaggcacca accagggcca
     9901 taaacagaaa tccgaccaat gctcctaacc acttccccgc ggtagatcct aaatcacttt
     9961 caaatcccga cacgggggca tcgggtatcc ggctcatcac ccagatgtat cgcgccattg
    10021 cccagacgaa atagccacac catgtataga aagcccacaa tgaatcgcca cggat
//
LOCUS       DADLSB010000135        10015 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.135, whole genome shotgun sequence.
ACCESSION   DADLSB010000135 DADLSB010000000
VERSION     DADLSB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.135"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..188)
                     /locus_tag="JN331_003187"
     CDS             complement(<1..188)
                     /locus_tag="JN331_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HAY7261261.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINART"
     gene            complement(193..378)
                     /locus_tag="JN331_003188"
     CDS             complement(193..378)
                     /locus_tag="JN331_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY7261262.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(375..3021)
                     /locus_tag="JN331_003189"
                     /pseudo
     CDS             complement(375..3021)
                     /locus_tag="JN331_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
     gene            3229..4218
                     /gene="ldhA"
                     /locus_tag="JN331_003190"
     CDS             3229..4218
                     /gene="ldhA"
                     /locus_tag="JN331_003190"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7261263.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4329..4751
                     /gene="hslJ"
                     /locus_tag="JN331_003191"
     CDS             4329..4751
                     /gene="hslJ"
                     /locus_tag="JN331_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY7261264.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4748..5014)
                     /locus_tag="JN331_003192"
     CDS             complement(4748..5014)
                     /locus_tag="JN331_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY7261265.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5288..8803
                     /gene="nifJ"
                     /locus_tag="JN331_003193"
                     /pseudo
     CDS             5288..8803
                     /gene="nifJ"
                     /locus_tag="JN331_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            9170..>10015
                     /gene="ompN"
                     /locus_tag="JN331_003194"
     CDS             9170..>10015
                     /gene="ompN"
                     /locus_tag="JN331_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HAY7261266.1"
                     /translation="MKRKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNERDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYVVAN
                     KTQNFE"
BASE COUNT         2547 a         2563 c         2455 g         2450 t
ORIGIN      
        1 tacgttcgcg cattaatttc ggttactaat ttctctgttt cagcatccgt tttcagcgca
       61 accagataac cataaaatgt ttcacctacc cgcccctgag ttctggcttc atccagagtc
      121 aatgccatta cattgctgct taccagccca acaagaaacg cacaaagaag taatgttttc
      181 ttcatcatcg cctcagaaaa gatcgctacg agtttcaagc agctcttcga catctttgtc
      241 tgccttgatg atgatctcat gctcaatttt aacgttcata ttgatagtga tcggttcctt
      301 aggtgcagcg acttcaatgc gaggcgtaca gccaaccagc ataaaagatg acgtcaacgc
      361 agccagtaaa attttcattg tttttcctca cactcttttc cttgcggaca gtcatttccc
      421 ggcagacgtg cgttctgctc caaccatgcc tggagattat cgccaaagcg taaactgcgc
      481 cacagcgtaa aaatattctc ttcatgatgg taattaaggt ttaccgtacc gcttttacca
      541 tcaacgcgac tggtacctgt aatgttggcc tgcatggtta ataaaccgag attatctaag
      601 ttaatttttg tcgatgaccg gctaatttcc atatagcgca accagttaat tgctgaaccc
      661 gcagtcatat tgtctttcac caccgcatct gcggtatctt tatccagccg caaggtcatt
      721 ggcccgctat ttgccagcca accatctttc actatccatt tttcgttgtt tagccataac
      781 ggcaatgcac cactaaatgc cccggacatg gcgaattgtt tcggattgac ggcgctaacc
      841 agttcgctgg atgagagatt attcagccgc aacagcgccg ggtcatgttg cggcatacgt
      901 aattgtttca tcagcacgtt accgcctaac acatcgacgc taacatcagt caacagcaag
      961 ggttcttctt cagtccacgg ataacgccct tgcaaatcag cagtaatatt tttcgctgtc
     1021 accagattaa tcacttcggc aattcgcaac gtaacggggc cgcgagtccc cagatgccag
     1081 gctccatcgg caaaacggaa aggcaggaca aaatcgacac cgttaacctg attatctggc
     1141 atccaggcac tgccgccttt caacacgccg tgccctcccg cgcggaatcc ttgttcaggc
     1201 gcagcagaaa atgcaacctg agcatacagt tcaccatcgc gtaagttcat cttccagtcg
     1261 ggtggcacca gcggctggaa tacagtcagt gattgttttg gccaccaggc gttaccgcgc
     1321 agacgaatac cgtcccagcg accatttacc cgaaccgggc caatctcacc agcatgtaaa
     1381 tcgcctttaa agaggaaata ggtcggatcg cgcccatcga cgctaaattt taaggttgat
     1441 ggcggtaaca cactgccgcc agtgaacagc gtttgcccgg cgtccagtga cagcgcgccg
     1501 ctgaaggagg gatgctgtgc gtcacgtacc cagacgacgg gcttgtcgag aattaatcgc
     1561 ggcttttcta ccgtcatcgt accgtattgt aactggtcga aaccggtgga gagatcggtc
     1621 agcgtaatcg tgctgtcatg ccactcacct tttcctgcga catcccaggt ggcattcatc
     1681 ggtgtaaaac tcccttttcc ccagtagcgc cactgccagc ggccagcgtc cagcgtcagg
     1741 gagaaaatca ttcgccagcc catccatatg cagcacgaaa tcgcccagtt cattttcatg
     1801 cgcctgcaaa atggcctgca aacgtccgtc aacaccacgt tgggtgactt ttacacccgc
     1861 taaaggccag cggatttcat cgatgtccag cgaatcgatg actcttccct ttgaacgaag
     1921 taacgcgcct ggctcaaagg tcagcgttgg gtcagacaga cttccactta gctgcgcagg
     1981 taaacgggca tataaaatga ggtccgcctg tttcgcttca ccggtcagct gcagaggcag
     2041 ttgactgtta tccatgctta attttcctgg gccaaaatta agcaccgcgt tgcccttacc
     2101 cgcttgcccc taggtcagca cactcagtcg tccgctgacc agagcgttct caagccctgc
     2161 ctgccagttg tcgactttga cacccagtcg gccactcaaa ggaaaacctg catacagcca
     2221 gctccagcga ccatcgctta cggtcaattg ttgacgagta atttgccacg gcaaatcgag
     2281 caacggatcg ccgttatccc gtgccagcac gatcaattgc ccgctatttt cctgccagtc
     2341 cagctcggca tccaccagtg acggttcctg cggcaagttt aacgtcgcag tagcatgccc
     2401 acttacagga agtccatccg gcacgagcgg catagtaaat tcccccacca gtttaaccgg
     2461 cggctgattt tcaaacgcga cgacatccag ttcgctgact gtaagttgtt gccctttcag
     2521 ctggccttga aatttaactt tttcgccctg ataacgcagt tgctggatat cagaggttaa
     2581 tgcgagagag agttttccct gccattcctg ccagggagaa aaaatcagtt tatcgatatt
     2641 gatccaggtg ttaggcagca tggactgcca ctgcgcgagg gtttttggag cagctggcga
     2701 ttgctccgtc tgcggcaatt tcgccagaca agcagaatca agttctaccg tgccgacgtt
     2761 caataaccag cggctgggat gtgaaaggct ggcgttggtg atatgcgcaa gctgacaatc
     2821 tcccaccaga taacggagat cgggaatgat taaaccttta cgcgtaatgc gtgggctttc
     2881 atctaatgca atacgtgtcc cgagcggtag ccagatgccc gccagcgtgg gaacccacag
     2941 cccgagcgtc atcagcagcg tcaacggcac aagaataatc agtaataaca gcgcgagaac
     3001 ggctttatat ttacccagca tgggtagtta acatcctgat ttagcgaaaa attaagcatt
     3061 caatacgggt attgtggcat gtttaaccgt tcagttgaag gttgcgccta cactaagcat
     3121 agttgttgat gaatttttca atatcgccat ggctttcaat tatatttgaa attttgtaaa
     3181 atatttttag tagcttaaat gtgattcaac atcactggag aaagtcttat gaaactcgcc
     3241 gtttatagca caaaacagta cgacaagaag tacctgcaac aggtgaacga gtcctttggc
     3301 tttgagctgg aattttttga ctttctgctg acggaaaaaa ccgctaaaac tgccaatggc
     3361 tgcgaagcgg tatgtatttt cgtaaacgat gacggcagcc gcccggtgct ggaagagctg
     3421 aaaaagcacg gcgttaaata tatcgccctg cgctgtgccg gtttcaataa cgtcgacctt
     3481 gatgcggcaa aagaactggg gctgaaagta gtccgtgttc cagcctatga tccagaggcc
     3541 gttgctgaac acgccatcgg tatgatgatg acgctgaacc gccgtattca ccgcgcgtat
     3601 cagcgtaccc gtgacgctaa cttctctctg gaaggtctga ccggctttac tatgtatggc
     3661 aaaacggcag gcgttatcgg taccggtaaa atcggtgtgg cgatgctgcg cattctgaaa
     3721 ggttttggta tgcgtctgct ggcgttcgat ccgtatccaa gtgcagcggc gctggaactc
     3781 ggtgtggagt atgtcgatct gccaaccctg ttctctgaat cagacgttat ctctctgcac
     3841 tgcccgctga caccggaaaa ctaccatctg ttgaacgaag ccgccttcga tcagatgaaa
     3901 aatggcgtga tgatcgtcaa taccagtcgc ggtgcattga ttgattctca ggcggcaatt
     3961 gaagcgctga aaaatcagaa aattggttcg ttgggtatgg acgtctatga gaacgaacgc
     4021 gatctattct ttgaagataa atccaacgac gtgatccagg atgacgtatt ccgtcgcctg
     4081 tctgcctgcc acaacgtgct gtttaccggg caccaggcat tcctgacagc agaagctctg
     4141 accagtattt ctcagactac gctgcaaaac ttaagcaatc tggaaaaagg tgaaacctgc
     4201 ccgaacgaac tggtttaatc ttgccgctcc cctgcaatcc aggggagctg attcagataa
     4261 tccccaatga cctttcattc tctattctta aaatagtcct gagtcagaaa ctgtaattga
     4321 gaaccacaat gaagaaagta gccgcgtttg ttgcgctaag cctgctgatg gcgggatgtg
     4381 taagtaatga caaaattgct gttacgccag aacagctaca gcatcatcgc tttgtgctgg
     4441 aaagcgtaaa cggtaagccc gtgaccagcg ataaaaatcc gccagaaatc agctttggtg
     4501 aaaaaatgat gatttccggc agcatgtgta accgctttag cggtgaaggc aaactgtcta
     4561 atggtgaact gacagccaaa gggctggcaa tgacccgtat gatgtgcgct aacccgcagc
     4621 ttaatgaact cgataacacc attagcgaaa tgctgaaaga aggtgcacaa gtggatctga
     4681 ccgcgaacca gttaacgctg gcgaccgcga aacagacatt aacttataag ctggcggatt
     4741 taatgaatta atagctgcca cagctcccgg cggcaagtga ctgttcgcta cagcgtttgc
     4801 cgttgggtaa tgcacacatc ccaatcgccg taccatccag ttgacgggca acagaaagcg
     4861 aaccgccgat cattgcacaa tttgcttctc cactactgga catcgacgct tttaaacctg
     4921 gcgctacgtg cgccgcagtc gcctgctgaa caggctcact actacacgcc gacaacaata
     4981 aagcggcaca ccctacccaa aacgctgctc gcatctcttc ttcctctgat cttcaagcca
     5041 aacgacaccg ccataaataa taggcagcac agagggcggc gtcgagagct gtcctgcgcg
     5101 ttgccccgca tttttacttt tttatggcta tttttttgcc ctctgtttga tcaaaacatt
     5161 cattactctg atgtggggga cacaaaagcg aaaatgcaga agaaagccat ttgctaaaat
     5221 tgaaggatta ctactgggcg cgcagcaatt tcgtgcgccc ctcatttgcg caatgtaagg
     5281 gtgtcatatg attactattg acggtaatgg cgcggttgct tcggtggcgt ttcgcaccag
     5341 tgaagttatc gccatctacc ctattacccc cagttccacg atggcagaac aggctgatgc
     5401 ctgggccgga aacggcttga agaacgtttg gggagacaca ccacgcgtgg ttgaaatgca
     5461 gtcggaagcg ggtgctatcg ctaccgtgca tggcgctttg cagacgggtg ccctttcaac
     5521 atcgtttacg tcatcgcagg gtttgctgct gatgatcccg acgatgtaca aactggcagg
     5581 cgaactgacg ccatttgtcc tgcatgtagc ggcacgtacc gttgccacac atgcactttc
     5641 tatttttggc gatcattccg acgttatggc ggtgcgccag acgggttgcg cgatgttgtg
     5701 tgcggctaac gtccaggaag cgcaagactt tgctctcatt tcgcacatcg caacgctgaa
     5761 gagccgcgtg ccatttattc atttctttga tggtttccgc acgtcccacg aaatcaataa
     5821 aattgtcccg ctggccgatg acactattct tgatctcatg ccgcaggccg aaattgatgc
     5881 tcatcgcgcc cgggcgctca acccggaaca tccggtgatc cgcggtacgt ccgccaatcc
     5941 tgacacttat ttccagtctc gcgaagccac caacccctgg tacaacgcgg tctatgacca
     6001 tgttgaacag gcgatgaatg atttcgctgc cgcgacaggt cgtcagtatc agccgtttga
     6061 gtattacggg catccgcaag cggaacgggt gattatcctg atgggctctg ccattggcac
     6121 ctgtgaagaa gtggttgatg aattgctaac ccgtggcgaa aaagttggcg tgctgaaagt
     6181 tcgtctgtac cgtcccttct ccgccaaaca cttgctgcaa gctctgccgg gatccgtacg
     6241 cagcgtggcg gtactggaca gaaccaaaga acccggtgcc caggcagaac cgctctatct
     6301 ggatgtgatg accgcactgg cagaagcctt taataatggt gagcgcgaaa ccctgccccg
     6361 tgtcattggt gggcgctatg gtctttcatc caaagaattt ggcccggact gtgtactggc
     6421 ggtatttgcc gagctcaacg cggctaaacc taaagcacgc tttacggttg gtatttacga
     6481 tgatgtgacc aatctgtcac tgccgttgcc ggaaaacact ctgccaaact cggcgaaact
     6541 tgaagccttg ttttatggcc ttggtagtga tggcagcgtt tccgcaacca aaaacaatat
     6601 caagattatc ggtaattcca cgccgtggta cgcacagggc tattttgttt acgactccaa
     6661 aaaagcgggc ggcctgacgg tttctcacct tcgcgtgagc gaacagccga ttcgttccgc
     6721 ttatctcatt tcctaggctg attttgttgg ctgccaccag ttgcagttta tcgataaata
     6781 tcagatggct gagcgtttaa aacctggcgg cattttcctg cttaatacgc cgtacagcgc
     6841 agatgaagtg tggtcgcgct tgccgcagga agttcaggcc gtgttaaacc agaaaaaagc
     6901 gcgcttctat gtgattaacg cggcgaaaat cgcccgcgaa tgtggcctgg cggcccgtat
     6961 taataccgtc atgcagatgg cttttttcca tctgacgcaa attctgcctg gcgatagcgc
     7021 cctcgcagaa ttgcagggtg cgattgccaa aagttacagt agcaaaggtc aggatctggt
     7081 ggagcgcaac tggcaggctc tggcgctggc gcgtgaatcc gtagaagaag ttccgttgca
     7141 accggtaaat ccgcacagcg ccaatcgacc gccagtggtt tccgatgccg cccctgattt
     7201 cgtgaaaacc gtaaccgcgg cgatgctcgc cgggcttggt gatgccctcc ccgtttcggc
     7261 gctgccgcca gacggcacct ggccgatggg caccactcgc tgggaaaaac gcaatatcgc
     7321 cgaagagatc cccatctgga aagaggaact ctgtacccaa tgtaaccact gcgtcgccgc
     7381 ttgcccacac tcagctattc gcgcaaaagt ggtaccgcct gaagcgatgg aaaacgcccc
     7441 tgccagcctg cattcgctag atgtgaaatc gcgtgatatg cgcgggcaga aatatgtctt
     7501 gcaggtggca ccggaagatt gcaccggttg taacctgtgc gtcgaagttt gcccggcgaa
     7561 agaccgtcag aatccagaga ttaaagccat caatatgatg tctcgcctgg aacatgtcga
     7621 agaagagaaa atcaattacg atttcttcct caacctgcca gaaatcgacc gtagcaaact
     7681 ggaacgtatt gatattcgta catcgcagct gattacaccg ctgtttgaat attcaggtgc
     7741 ttgctccggt tgtggcgaga cgccgtatat taaattgctg actcagctct atggtgaccg
     7801 gatgttgatc gctaacgcca ccggctgttc ttcaatttat ggcggtaacc tgccctctac
     7861 accgtatacc accgatgcca acggtcgtgg gccggcatgg gcgaactctc tatttgaaga
     7921 taatgccgaa tttggccttg gtttccgcct gacggtcgat caacaccgtg tccgcgtgct
     7981 gcgtctgctg gatcaatttg ccgataaaat cccggcggaa ttactgacgg cgttgaaatc
     8041 agacgctacg ccagaggttc gtcgagaaca ggtcgcagct ttacgccagc aactcaacga
     8101 tgttgccgaa gcacatgaac tgctgcgtga cgcagatgca ctggtggaaa aatcaatctg
     8161 gctgattggt ggcgacggct gggcttacga tatcggcttt ggcggtctgg atcatgtatt
     8221 gagtttgacg gaaaacgtca acattctggt gctggatacg caatgctatt ccaacaccgg
     8281 tggtcaggcg tcgaaagcga caccgctggg tgcagtaact aagttcggcg agcacggcaa
     8341 acgtaaagat cttggcgtca gtatgatgat gtacggtcat gtttatgtgg cgcagatttc
     8401 tctcggcgcg cagctgaacc agacggtgaa agcgattcag gaagcggaag cgtatccggg
     8461 accatcgctg atcattgctt atagcccgtg tgaagagcat ggttacgatc tggctctcag
     8521 ccacgaccag atgcgccaac tcactgctac cggcttctgg ccgctatatc gctttgatcc
     8581 gcgtcgtgcc gatgaaggca aactgccgct ggcgctggat tcacgcccgc cgtcagaagc
     8641 actggaagaa acgttacttc acgagcagcg tttccgtcgt ctgaattcgc agcagccaga
     8701 agtggcagaa cagttatgga aagatgctgc agctgatttg caaaaacgct atgacttcct
     8761 ggcacaaatg gccggaaaag cggaaaaaag caacaccgat taaatgctct ggataaggat
     8821 tatccaattc taaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt atatgttgga
     8881 aaatcagtgg caatgcaatg gcccaacaga aaataaaatc tgtgacaata aaggcatata
     8941 acccgcgcag aataacgtat agaaaattaa aagtataatt tttattttgt atgtattatc
     9001 ttcgatacat tcaccagact tattatcatt tcagaggaat tatttgatta aggttttact
     9061 taaggcgtaa caaatgatat ttatcggcta actgaacttc tcctttagga tgttttcact
     9121 ccccgaaatg gggataaaag gcaaataaaa taactaaagg atttattcaa tgaaaagaaa
     9181 agtactggca cttttaattc ctgccctgct cgccgcaggt gctgcacatg cagccgaagt
     9241 ttataataaa gacggcaaca aattagatct gtatggcaaa gttgatggcc tgcattattt
     9301 ttctgataat tcagcgaaag atggcgacca gagctatgcg cgtctgggtt ttaaaggcga
     9361 aacccaaatt aacgatcaac ttactggcta cggtcaatgg gaatacaata ttcaggcaaa
     9421 caacactgaa tcttcaaaaa accagtcatg gacccgtctg gcatttgccg gactgaaatt
     9481 tgcagattac ggttctttcg attacggacg taattatggc gtaatgtacg acatcgaagg
     9541 ctggaccgat atgctgcctg aatttggcgg tgactcttat accaatgcag acaactttat
     9601 gactggtcga gccaatggcg tcgcgactta tcgtaatact gatttcttcg gtctggtaaa
     9661 tggtctgaac ttcgcggtgc agtatcaagg taacaacgaa ggagccagta atggtcagga
     9721 aggcaccaat aacgaacgcg atgttcgtca tgaaaacggt gacggctggg gtctttccac
     9781 aacatatgat ttaggcatgg gctttagcgc tggtgcggca tacacctctt ctgaccgcac
     9841 caatgaccag gttaaccata ctgcggcggg tggtgataaa gcagacgcgt ggactgctgg
     9901 gctaaaatac gatgctaaca atatttacct agcaaccatg tattcagaaa cgcgtaatat
     9961 gaccccgttt ggcgacagcg attatgtagt tgcgaacaaa acccagaact ttgaa
//
LOCUS       DADLSB010000136         9873 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.136, whole genome shotgun sequence.
ACCESSION   DADLSB010000136 DADLSB010000000
VERSION     DADLSB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.136"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..518
                     /locus_tag="JN331_003195"
     CDS             <1..518
                     /locus_tag="JN331_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY7261267.1"
                     /translation="SILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVKAT
                     GSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSWLVQPVRSEET
                     LHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYKSA
                     ESVVQCLGRPD"
     gene            634..1194
                     /locus_tag="JN331_003196"
                     /pseudo
     CDS             634..1194
                     /locus_tag="JN331_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1251..2708)
                     /gene="pepD"
                     /locus_tag="JN331_003197"
     CDS             complement(1251..2708)
                     /gene="pepD"
                     /locus_tag="JN331_003197"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7261268.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2970..3428
                     /gene="gpt"
                     /locus_tag="JN331_003198"
     CDS             2970..3428
                     /gene="gpt"
                     /locus_tag="JN331_003198"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7261269.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3520..4764
                     /gene="frsA"
                     /locus_tag="JN331_003199"
     CDS             3520..4764
                     /gene="frsA"
                     /locus_tag="JN331_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7261270.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4822..5223
                     /locus_tag="JN331_003200"
     CDS             4822..5223
                     /locus_tag="JN331_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7261271.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5262..6317)
                     /gene="phoE"
                     /locus_tag="JN331_003201"
     CDS             complement(5262..6317)
                     /gene="phoE"
                     /locus_tag="JN331_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7261272.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            6605..7708
                     /gene="proB"
                     /locus_tag="JN331_003202"
     CDS             6605..7708
                     /gene="proB"
                     /locus_tag="JN331_003202"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7261273.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7720..8973
                     /gene="proA"
                     /locus_tag="JN331_003203"
     CDS             7720..8973
                     /gene="proA"
                     /locus_tag="JN331_003203"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7261274.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIVEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9088..9163
                     /locus_tag="JN331_003204"
     tRNA            9088..9163
                     /locus_tag="JN331_003204"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9121..9123,aa:Thr,seq:cgt)
     gene            complement(9178..>9873)
                     /locus_tag="JN331_003205"
     CDS             complement(9178..>9873)
                     /locus_tag="JN331_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7261275.1"
                     /translation="LRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPE
                     PLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRV
                     LKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDL
                     RHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPN
                     LSQSKNKEGTNDV"
BASE COUNT         2363 a         2458 c         2589 g         2463 t
ORIGIN      
        1 agtctatttt acagcggcat attgcctcgt tttcgcatca tggtttgcct gaaggcagtg
       61 acgacgcgct ggcgtttgcg cgttttaatc gccacctgat cgctttgcgc ataatgcaac
      121 aggttaaggc caccggaagt ccggttctgg atgtggcgca taactttgtt agcgcgtgtc
      181 gaatcggtga tcaacaaggg gtcttacatc gtaaaggtgc cacgcctgat gactgtggtc
      241 tggtggttat tcccggttca cgcggtgatt actcctggct ggttcagccc gtcaggagtg
      301 aggaaacatt gcattcgctg gcgcatgggg ctgggcgcaa atgggggcgc accgagtgta
      361 aagggcgtct ggcagcgaaa tacacagcga cgcaactctc gcgtactgaa cttggcagtc
      421 gggtaatttg tcgcgataaa caactcatct tcgaagaagc gccacaagcc tataaatcgg
      481 ctgaaagtgt ggtgcaatgt ctgggcaggc cggattaatt attcctgtcg cgcggctgcg
      541 tccggtgcta acgctcaaaa acagtggagg gaaaaaagga tgatcttgct ataactctcc
      601 tcagcgcagg ggccggaaga atgttgtctc gcagtgaaaa aggcgctgga caggctaatt
      661 aaagaagctg cccgacaaga cgtcgcggta acggtgctgg aaacagaaac aggccgctac
      721 tctgacacgc tgcgttcggc gctgatttct ctggatggcg acaacgcatg ggcgttaagc
      781 gaaagctggt gtggcactat tcagtggatt tgcccgagcc cgtatcgccc acatcacggg
      841 cgtaaaaact ggtttctggg cattgggcgt tttaccgctg atgagcagga gcaatccgat
      901 gcgatccggt atgagacgct gcgttcgtcg gggccgggcg gtcaacatgt caataaaacc
      961 gactcggcgg tacgcgccac gcatttggca tccggtatta gcgtgaaggt tcagtcagag
     1021 cgcagccagc atgctaacaa acggctggcg cgattgctga ttgcctggaa gctggatcag
     1081 cagcaatagg aaaatagcgc ggtgctgaaa tcacagcggc gaatgttcca tcaccagatt
     1141 gaacgtggca acccgcgacg aacgtttaca gggatggctt ttatcgaagg ataatgaagg
     1201 atgaaactgc cggaaggcga ttaaacgcca tccggcagca aatcaaataa ttacttcgcc
     1261 ggaatttctt tcagcagttc agtcagcagt gtccagtaat gacctacgct ttcgatgtga
     1321 acttgctcat ccggagagtg tggaccggtg atagttggcc cgatagaaac catgtccatt
     1381 tccggatacg gttttttgaa cagaccacat tccaggcccg cgtggataat ctggatgttc
     1441 ggcgtcttgt tgaacaggcg ctgataggtt tcacgtacca gatgcatcac cggagaatta
     1501 gcgtccggct gccagccagg atatgcgcct ttcgcttcgg ttttcgcgcc agccagttta
     1561 cccagcgaat ccagcatgct caccacgtag tctttaccgc tgtcgatcag tgaacggatc
     1621 aggcagtgaa tttctacgtt attgtcagtc atggtcacca caccgacgtt cagggaggtt
     1681 tcgaccacac cttttgccac gtcggaattg cggatcacac cgttcggggt ggcgttcagc
     1741 agacgaataa aggtatcgcg agatttcgca atcagggcag ctttatcgtt cgctacagag
     1801 tccagcaaca aggccagatt tttctctttc tctgccagct cgtttttcag gatctcctga
     1861 taggtattca ccagagattt cagtgcgtcg actttatcag ctgcgacagc aatggtcgca
     1921 aaggcttcac gcgggatggc gttacgcagt gtgccgccgt tgaaatcgat aaggcgcagg
     1981 tccagttctt ctgcatgacc cgccaggaag cgcaccagca gtttgttggc attacccagg
     2041 ccaacgtgga tttcaccgcc ggagtgaccg cctttcagac cttttaaggt taacttgaag
     2101 gtttcaaaac cagctggaac cgcttcacga tctaaatgca ggttggaggt gaagtcgata
     2161 ccccccgcac aacccatgta gatttcacct tcttcttcgg agtcggtgtt aatcagaata
     2221 tcagcctgca accagttgct ctgtaagccg aacgcaccgt ccataccggc ttcttcggtc
     2281 atggtcagca gcacttccag cgggccgtga accacgtttt cgtcagccag aaccgccagt
     2341 gcagaggcca taccaatgcc gttatccgca cccagcgtgg tgccgcgcgc tttaacccat
     2401 tcgccatcaa tataaggctg gataggatct ttcgtgaagt catgcacggt gtcgttattt
     2461 ttctgcggca ccatatcgag gtgggcctgt aagacgaccg gtttacgatt ttccatacct
     2521 gcggtagcag gtttacgaat caggatatta cctacctgat cgcgttcgac atggaaacct
     2581 ttctcttttg cccaaccaac aatgtattca gcgagttgct cttcatgata ggacgggtga
     2641 ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa ttgagacagt
     2701 tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg tcacagggtt
     2761 agcagaaaat gttgtcaaca caagacaggc ttgcgagata tgtattgaga ataccacttt
     2821 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     2881 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     2941 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     3001 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     3061 gcattattgc cgtaagccgt ggcggtctgg taccgggtgc gttactggcg cgtgaactgg
     3121 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     3181 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     3241 tggataccgg tggtactgct gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     3301 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     3361 aagatacctg gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     3421 gtcgctaatc ttttcaacgc ctggcattgc cgggcgttgt tctttttaac ttcaggcagg
     3481 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     3541 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     3601 aatcacggcg cacaaccgcc tgtgcagtcg gctcttgatg gtaaaaccat ccctcactgg
     3661 tatcgcatga ttaaccgtct gatgtggatc tggcgcggta ttgacccacg tgaaatcctc
     3721 gacgtccagg cacgtattgt gatgagcgat gctgaacgta ccgacgatga tttatacgat
     3781 accgtgattg gctaccgtgg tggcaactgg atttatgagt gggccaccca ggcgatggtg
     3841 tggcaacaaa aagcctgtac ggaagaagat ccgcaactca gtggtcgaca ctggctgcat
     3901 gcggctacgt tgtacaacat tgccgcctat cctcatctga aaggagatga cctggccgag
     3961 caagcgcagg ctttatcaaa ccgcgcctat gaagaggccg cccagcgtct gccgggcacg
     4021 atgcggcaga tggagtttac cgtacccggc ggtgcgccca tcaccggctt tttgcatatg
     4081 ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag
     4141 acggactatt acagcctgta tgaacgttat tttgcgccgc gcggcattgc gatgctgact
     4201 attgatatgc cgtcggtggg cttttcttca aaatggaagc tcacccagga ctccagcctg
     4261 ttgcatcagc acgtcttaaa ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc
     4321 gcggcctttg gtttccgttt cggcgctaac gttgccgtgc gtctggcata ccttgaatcg
     4381 ccgcgtctga aagcggttgc ctgtcttggt ccggtagttc ataccctgtt gagtgatttt
     4441 aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc tggcgagtcg tttggggatg
     4501 catgatgctt ccgacgaagc gttacgcgtg gagctgaatc gctattcatt aaaagtgcaa
     4561 ggattgctgg ggcgtcgctg cccaacgcca atgttatcag gctactggaa gaacgatccg
     4621 ttcagcccgg aagaggactc acgcttaatc acctcatcat ctgctgacgg taaattatta
     4681 gagatcccat ttaacccggt gtatcggaat tttgacaaag gtcttcagga aatcaccggc
     4741 tggatcgaaa aacgcttgtg ttaaaaattt gctaaatttt gccaatttgg taaaacagtt
     4801 gcatcacaac aggagatagc aatgacgtta ccgagtggac acccgaagag cagattgatc
     4861 aaaaaattta ccgcactagg cccgtatatt cgtgaaggta agtgcgaaga taatcgattc
     4921 tttttcgatt gtctggctgt atgcgtcaac gtgaaaccgg caccggaagt gcgtgaattc
     4981 tggggctggt ggatggagct ggaagcgcag gaatcccgtt ttacctacag ctaccagttt
     5041 ggtctgttcg ataaagcagg cgactggaag agtgttccgg taaaagatac tgaagtggtt
     5101 gaacgactgg agcacaccct gcgtgagttt cacgagaagc tgcgtgaact gctgacgacg
     5161 ctgaatctga agctggaacc ggcggatgat tttcgtgatg aaccggtgaa gttaacggcg
     5221 tgagtgaaat gtgccggatg catcacatcc ggcaatattc attaaaactg atacgtcatc
     5281 ccaaccgcga cagtatcatc attattaata ttaagcttat tatcgctatc cagttggttg
     5341 attttataat caacaaacgc tgacatattt ttgttgaaat aatacgtagc accgacgtcg
     5401 atataattga ccagatcttc atcaccgatg ccctcaatat ctttcccttt cgataagaca
     5461 taacccagcg atggacgcag accaaagtca aactggtatt gagcgaccgc ttcaaagttc
     5521 tgtgtcttat tggcaaagcc gccagttatt ggcgtcattt tgcgtgtttc agaatagaaa
     5581 gttgccagat aaatattatt ggcatcgtat ttcagacctg ttgcccatgc ttctgcacgc
     5641 ttgcctgtgc cacggctttg caggttctgc tcgttggtgc gatctgagtt ggtataggcc
     5701 ccactaatgg cgaaatcgct gccgccaaag tcatatgtca atgacgtgcc gaagccatcg
     5761 ccgttttgct ttttaacgtc gcggttttcg tttttccctt gatattgcag ggttaagttc
     5821 aggccatcga taacgccgaa gaagtcggtg ttccgatacg tcgccagacc gctggcgcgt
     5881 ttggtcataa agttgtcggt ctgcgccgag gaatcgccac caaattccgg gaacatatcg
     5941 gtccaggctt ccacgtcata caacgcgccc aggttacgac cataatcgaa agaacccaaa
     6001 tctttatatt tcaacccggc aaaagcgaga cgcgtttttt gctgtgcagt atcactctcc
     6061 gctttatttc cggcaaactc cgcttcccac cgaccataac cagtcagttg atcgttaatt
     6121 tgagtttcac ctttaaaacc aaaacggata taactctggt cgccatcttt actggcgtta
     6181 tcactcatat aatgcatggc tttaactttg ccatagacat ccagtttatt accgtcttta
     6241 ttatatattt ctgcagcctg tacagatgca gatgccacaa tgcccatcac cactaatgcc
     6301 agagtgctct ttttcatttt cattcctgat tttaattaac gcgcgaatat tcagcgggag
     6361 agccccgttg aaaacaggaa agtttttaac ctgagattgt taaagatata ttacagatta
     6421 atgatattct gaaaatgtgg taatttatta aatctgtaat aaaagcgtaa acaactgccg
     6481 ctacgcttgc tgatcccgcg caacaaaacg ccatgctttg ctcgcagatg gttggcaacc
     6541 gacgacagtc ctgctaaaac gttcgtttga tatcattttt cctaaaattg aatggcagag
     6601 aatcatgagt gacagccaga cgctggtggt aaaactcggc accagtgtgc taacaggcgg
     6661 atcgcgccgt ctgaaccgtg cccatatcgt tgaacttgtt cgccagtgcg cgcagttaca
     6721 tgccgccggg catcggattg ttattgtgac gtcgggcgcg atcgccgccg gacgtgagca
     6781 cctgggttac ccggaactgc cagcgactat cgcctcgaaa caactgctgg cggcggtagg
     6841 gcagagtcga ctgattcaac tgtgggaaca gctgttttcg atttatggca ttcacgtcgg
     6901 gcaaatgctg ctgacccgtg ctgatatgga agaccgtgaa cgcttcctga acgcccgcga
     6961 caccctgcgt gcgttgctcg ataacaatat cgttccggta atcaatgaga acgatgctgt
     7021 cgctacggca gagattaagg tcggcgataa cgataacctt tctgcgctgg cggcgattct
     7081 ggcgggtgcc gataaactgt tgctgctgac cgatcaaaaa ggtttgtata ccgctgatcc
     7141 gcgcagcaat ccgcaggcag aactgattaa agatgtttac ggcattgatg acgcactgcg
     7201 cgcgattgcc ggtgacagcg tttcaggcct cggaactggc ggcatgagta ccaaattgca
     7261 ggccgctgac gtggcttgcc gtgcgggtat cgacaccatt attgccgcgg gcagcaagcc
     7321 gggcgttatt ggtgatgtga tggaaggcat ttccgtcggt acgctgttcc atgcccaggc
     7381 gactccgctt gaaaaccgta aacgctggat tttcggtgcg ccgccggcgg gtgaaatcac
     7441 ggtagatgaa ggggcaactg ccgccattct ggaacgcggc agctccctgt tgccgaaagg
     7501 cattaaaagc gtgactggca atttctcgcg tggtgaagtc atccgcattt gcaacctcga
     7561 aggtcgcgat atcgcccacg gtgtcagtcg ttacaacagc gatgcattac gccgtattgc
     7621 cggacaccac tcgcaagaaa ttgatgcaat actgggatat gaatacggcc cggttgccgt
     7681 tcaccgtgat gacatgatta cccgttaagg agctggccga tgctggaaca aatgggcatt
     7741 gccgcgaagc aagcctcgta taaattagcg caactctcca gccgcgaaaa aaatcgcgtg
     7801 ctggaaaaaa tcgccgatga actggaagca caaagcgaaa tcatcctcaa cgctaacgcc
     7861 caggatgttg ctgacgcgcg agccaatggc cttagcgaag cgatgcttga ccgtctggca
     7921 ctgacgcccg cacggctgaa aggcattgcc gacgatgtac gtcaggtgtg caacctcgcc
     7981 gatccggtgg ggcaggtaat cgatggcggc gtactggaca gcggcctgcg tcttgagcgt
     8041 cgtcgcgtac cgctgggggt tattggcgtg atttatgaag cgcgcccgaa cgtgacggtt
     8101 gatgtcgctt cgctgtgcct gaaaaccggt aatgcggtga tcctgcgtgg tggtaaagaa
     8161 acctgtcgca ctaacgcggc aacggtggcg gtgattcagg acgccctgaa atcctgtggc
     8221 ttaccggcgg gtgccgtgca ggcgattgat aatcctgacc gtgcgctggt cagtgaaatg
     8281 ctgcgtatgg ataaatacat cgacatgctg atcccgcgtg gtggcgctgg tttgcataaa
     8341 ctgtgccgtg aacagtcgac aatcccggtg atcacaggtg gtataggcgt atgccatatt
     8401 tacgttgatg aaagtgcaga gatcgttgaa gcattaaaag tgatcgtcaa tgcgaaaact
     8461 cagcgtccga gcacatgtaa tacggttgaa acgttgctgg tgaataaaaa catcgccgat
     8521 agcttcctgc ccgcattaag caaacaaatg gcggaaagcg gcgtgacatt acacgcagat
     8581 gcagctgcgc tgacacagtt gcaggcaggc cctgcgaagg tggtggcggt taaagccgaa
     8641 gagtatgacg atgagtttct gtcattagat ttgaacgtca aaatcgtcag cgatcttgac
     8701 gatgccatcg cccatattcg tgaacacggc acacaacact ccgatgcgat cctgacccgc
     8761 gatatgcgca acgcccagcg ttttgttaac gaagtggatt cgtccgctgt ttacgttaac
     8821 gcctctacgc gttttaccga cggcggccag tttggtctgg gtgcggaagt ggcggtaagc
     8881 acacaaaaac tccacgcgcg tggcccaatg gggctggaag cactgaccac ttacaagtgg
     8941 atcggcattg gtgattacac cattcgtgcg taaataaaac cgggtgatgc aaaagtagcc
     9001 atttgattca caaggccatt gacgcatcgc ccggttagtt ttaaccttgt ccaccgtgat
     9061 tcacgttcgt gaacatgtcc tttcagggcc gatatagctc agttggtaga gcagcgcatt
     9121 cgtaatgcga aggtcgtagg ttcgactcct attatcggca ccattaaaat caataagtta
     9181 cacatcatta gtaccttcct tattttttga ctgggacaaa tttgggaccg atgggttcag
     9241 gatcgagtct atttgccgtg cgtgttcggt aaggtgatta ggtgcaaggt gagcatatcg
     9301 acgaaccatt tcgatagact cccagcctcc catttcctgt aacactgaca acgggactcc
     9361 ggcttgaacc agccaacttg cccaggtgtg tctcaagtcg tgaaatctga aatcatcaat
     9421 accagcccgt ctcagcgccg ctttccaggc tgtgtttgcg tcataccgca tcttccttac
     9481 tgttggcgct ttcgttccgt ctggtttggt acagctttcc ttgtacacaa atacccaacg
     9541 gtgatgattc ccgatttgtt ttttcaaaac gcgacatgca gtatcattca gcgcaacgcc
     9601 gattgcgcgg tttgatttac tctcttccgg gtttatccat gccacccggc gctgcatatc
     9661 tatttgttgc cattcaaggt tgatgatgtt cgagcgtctt aagcctgttg ccagtgcaaa
     9721 ttcaacaaca gactttaatg gctccggaca ttcatcaatc agcctttgtg cttcatgggg
     9781 ctccagccag cggatccgtt tattctttgg ttgaggcact ttaataattg gtgccttatc
     9841 cagcattttc cattcacgct ctgcggctct tag
//
LOCUS       DADLSB010000137         9853 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.137, whole genome shotgun sequence.
ACCESSION   DADLSB010000137 DADLSB010000000
VERSION     DADLSB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.137"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            372..1493
                     /gene="ompC"
                     /locus_tag="JN331_003206"
     CDS             372..1493
                     /gene="ompC"
                     /locus_tag="JN331_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7261276.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1605..2660
                     /gene="apbE"
                     /locus_tag="JN331_003207"
     CDS             1605..2660
                     /gene="apbE"
                     /locus_tag="JN331_003207"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY7261277.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2734..3798
                     /gene="ada"
                     /locus_tag="JN331_003208"
     CDS             2734..3798
                     /gene="ada"
                     /locus_tag="JN331_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY7261278.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3798..4448
                     /gene="alkB"
                     /locus_tag="JN331_003209"
     CDS             3798..4448
                     /gene="alkB"
                     /locus_tag="JN331_003209"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAY7261279.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     EATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4524..6167
                     /gene="yojI"
                     /locus_tag="JN331_003210"
     CDS             4524..6167
                     /gene="yojI"
                     /locus_tag="JN331_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY7261280.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6385..8031
                     /gene="mqo"
                     /locus_tag="JN331_003211"
     CDS             6385..8031
                     /gene="mqo"
                     /locus_tag="JN331_003211"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HAY7261281.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGGAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNRHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGALWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8181..8669)
                     /gene="eco"
                     /locus_tag="JN331_003212"
     CDS             complement(8181..8669)
                     /gene="eco"
                     /locus_tag="JN331_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY7261282.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(8754..8969)
                     /locus_tag="JN331_003213"
     CDS             complement(8754..8969)
                     /locus_tag="JN331_003213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261283.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAIIFTRD
                     ISAGLLPHQNLTLKEQVKLSECSLSYS"
     gene            9078..9572
                     /gene="napF"
                     /locus_tag="JN331_003214"
     CDS             9078..9572
                     /gene="napF"
                     /locus_tag="JN331_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY7261284.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9562..9825
                     /gene="napD"
                     /locus_tag="JN331_003215"
     CDS             9562..9825
                     /gene="napD"
                     /locus_tag="JN331_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY7261285.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQQNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
BASE COUNT         2393 a         2424 c         2630 g         2406 t
ORIGIN      
        1 atgaatgacg gtaataaata aagttaatga tgatagcggg agttattcta gttgcgagtg
       61 aaggttttgt tttgacattc agtgctgtca aatacttaag aataagttat tgattttaac
      121 cttgaattat tattgcttga tgttaggtgc ttatttcgcc attccgcaat aatcttaaaa
      181 agttcacttg catttacatt ttgaaacatc tatagcgata aatgaaacat cttaaaagtt
      241 ttagtatcat attcgtgttg gattattctg catttttggg gagaatggac ttgccgactg
      301 attaatgagg gttaatcagt atgcagtggc ataaaaaagc aaataaaggc atataacaga
      361 gggttaataa catgaaagtt aaagtactgt ccctcctggt cccagctctg ctggtagcag
      421 gcgcagcaaa cgctgctgaa gtttacaaca aagacggcaa caaattagat ctgtacggta
      481 aagtagacgg cctgcactat ttctctgacg acaagtctgt agatggcgac cagacctaca
      541 tgcgtcttgg cttcaaaggt gaaactcagg ttactgacca gctgaccggt tacggccagt
      601 gggaatatca gatccagggc aacagcgctg aaaacgaaaa caactcctgg acccgtgtgg
      661 cattcgcagg tctgaaattc caggatgtag gttctttcga ctacggtcgt aactacggcg
      721 tagtttacga cgtaacttcc tggaccgacg tactgccaga attcggtggt gacacttacg
      781 gttctgacaa cttcatgcag cagcgtggta acggcttcgc aacctaccgt agcaccgact
      841 tctttggtct ggttgacggt ctgaacttcg ctgttcagta tcagggcaaa aacggtagcc
      901 cggaaggtga aggcatgacc aacaacggtc gtgaagctct gcgtcagaac ggtgacggcg
      961 ttggcggttc tattacttat gattacgaag gcttcggtat cggtgctgca gtttccagct
     1021 ccaaacgtac tgatgaccag aacttcggtc tgaaccgtta tgatgagcgt tacatcggta
     1081 atggcgaccg cgctgaaacc tacaccggtg gtctgaaata cgacgcgaac aacatctacc
     1141 tagctgctca gtacacccag acctacaacg caactcgcgt aggcaacttg ggttgggcga
     1201 acaaagcaca gaacttcgaa gctgttgctc agtaccagtt cgacttcggt ctgcgtccgt
     1261 ccctggctta cctgcagtct aaaggtaaaa acctgggtgt catcaatggt cgtaactacg
     1321 acgacgaaga tatcctgaaa tatgttgatg ttggtgcgac ctactacttc aacaaaaaca
     1381 tgtccaccta cgttgactac aaaatcaacc tgctggacga caaccagttc actcgtgacg
     1441 ctggcatcaa cactgataac atcgtagctt tgggtctggt ttaccagttc taatctcgat
     1501 ggatatcgaa caaagggcct gcgggccctt ttttcattgt tttcagcgta caaacacagt
     1561 tttttggtgt actcttgcga ccgttcgcat gaggataatc acgtatggaa ataagcttta
     1621 cccgcgtggc actgctggtt gccgcgctct tctttgttgg ttgcgatcaa aaaccacaac
     1681 ccgccaaaac ccacgctact gaagttactg ttcttgaagg caaaaccatg ggtaccttct
     1741 ggcgtgccag catcccgggc attgatgcca aacgcagtgc cgaacttaaa gaaaagattc
     1801 agacccagct ggacgccgac gatcagctgc tttcgaccta taaaaaagat tccgcgctga
     1861 tgcgctttaa cgactcgcaa agtttgtcgc cgtggccggt aagtgaagcg atggccgata
     1921 tcgtcaccac ctcgctgcgc attggggcaa ggaccgatgg tgcgatggat attaccgtcg
     1981 ggccgctggt gaatctgtgg ggctttggcc cggaacaaca gccggttcaa attccgagcc
     2041 aggaacagat cgatgcgatg aaagccaaaa ccggcttaca gcacttgacg gtcatcaatc
     2101 agtcgcatca gcaatatctg caaaaagacc tgccggattt atatgtcgat ctctccaccg
     2161 tcggtgaagg ttatgcggcg gatcacctgg cacgcttgat ggagcaggaa gggatttccc
     2221 gctatctggt gtcggtgggc ggcgcgctga acagccgtgg tatgaacggt gaaggccagc
     2281 cgtggcgggt agcgattcaa aaaccaaccg ataaagaaaa cgcggttcag gctgtggtgg
     2341 atatcaacgg tcatgggatt agcacctctg gcagctaccg taactattac gaactggacg
     2401 gcaaacgtct ttcccatgtt atcgatccgc aaaccgggcg tcccatcgaa cacaatctgg
     2461 tatccgtgac ggtgattgct ccgacggcgc tggaagccga tgcctgggat actggcttga
     2521 tggtactcgg gccggagaaa gccaaagagg ttgttcgccg ggaagggctg gcggtttata
     2581 tgatcaccaa agaaggcgat agctttaaaa cctggatgtc accacagttt aaaagcttcc
     2641 ttgtcagcga aaaaaattaa agcgcaagat tgttggtttt tgcgtgatgg tgaccgggca
     2701 gactaaaggc tatccttaac cagggagctg attatgaaaa acgccacatg cttaactgac
     2761 gatcaacgct ggcaatctgt cttagcccgc gacccgaatg ccgacggcga attcgttttc
     2821 gccgtgcgca ccacgggtat cttttgccgt ccgtcttgcc gcgccagaca tgccttgcgg
     2881 gaaaatgtct ccttctacgc aaatgccagc gaggcactcg ccgccggatt tcgcccctgc
     2941 aaacgttgtc agccagacaa agccaatccc cggcaacatc ggttggataa aatcacccac
     3001 gcgtgtcgac tgctggaaca ggaaacgcct gtaacgctgg aagccttagc cgaccaggtg
     3061 gcgatgagtc catttcatct acatcggttg tttaaagcga ctaccggaat gacgcctaaa
     3121 gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat cgctggcgaa aggggagagc
     3181 gtgacgacgt ctattcttaa cgccggattc cccgacagca gcagttacta ccgcaaagcc
     3241 gatgaaacgc tgggcatgac ggctaaacaa ttccgtcacg gtggcgaaaa tctggcggtg
     3301 cgttacgcgc tggctgattg tgagctgggt cgttgcctgg tggcagaaag cgagcggggg
     3361 atttgcgcga tattgctggg cgatgatgac gcgacattaa tcagcgagtt gcagcagatg
     3421 tttcccgctg ccgacaacgc gcctgccgat ctgacgtttc agcaacatgt gcgtgaagtg
     3481 atcgccagcc tcaatcaacg cgatacgccg ctgacgttac cgctggatat tcgcggcact
     3541 gcttttcagc aacaagtctg gcaggcactg cgcacgatac cttgtggtga aaccgtcagt
     3601 tatcagcaac tggctaacgc catcggcaaa ccgaaagcgg tacgggccgt tgccagcgcc
     3661 tgtgccgcca acaagctggc tatcgtaata ccctgtcatc gagtggtccg tggtgatggc
     3721 acactttccg gttaccgctg gggcgtgtcg cgtaaagcgc aactgctgcg ccgcgaagct
     3781 gaaaatgagg agaggtaatg ttggatctgt ttgccgatgc tgaaccgtgg caagagccac
     3841 tggcggctgg tgcggtaatt ttacggcgtt ttgcttttaa tgctgcggag caactaatcc
     3901 gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca gatggtcgct ccaggcggat
     3961 ataccatgtc ggtggcgatg accaactgtg ggcatctggg ctggacgacc catcggcaag
     4021 gttatctcta ttcgcccatt gatccgcaaa caaataaacc gtggcccgcc atgccacaaa
     4081 gctttcatga tttatgtcaa cgtgaggcta cggcggcggg ctatccagat ttccagccag
     4141 atgcttgtct tatcaaccgc tacgctcctg gcgcgaaact gtcgctgcat caggataaag
     4201 acgaaccgga tctgcgcgcg ccaattgttt ctgtttctct gggcttaccc gcgatttttc
     4261 aatttggtgg cctgaaacgt aatgatccgc tcaaacgttt gttgttggaa catggcgatg
     4321 tggtggtatg gggcggtgaa tcgcggctgt tttatcacgg tattcaaccg ttgaaagcgg
     4381 ggtttcatcc actcaccacc gactgccgct acaacctgac attccgtcag gcaggtaaaa
     4441 aagaataaaa ataagaatta ttattgctgt gcgcgcgaag atgtttaaac tgcgggctgt
     4501 aattcattgt ccgggttttc tgcatggaac ttcttgtact tgtctggcgg cagtatcgct
     4561 ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc ggcattaggc attggcttaa
     4621 ttgcttttat caatcagcgc cttatcgaaa cggcggatac cagtctgctg gtgttaccgg
     4681 agtttctggg attattgctg ctgttgatgg cagtcactct cggatcgcaa ctggcgctca
     4741 ccactttggg gcatcacttc gtttaccgac tgcgtagtga atttatcaag cggattctgg
     4801 atacccacgt cgagcgcatt gaacaactcg gtagcgcctc gttgctggcg ggattaacca
     4861 gcgatgtgcg caatatcacc attgcttttg tgcgtctgcc ggaactgggg caggggatca
     4921 ttctcactat cggttcatcg gcgtatctgt ggatgctgtc gggcaaaatg ttgctggtaa
     4981 cggctatctg gatggcgatc accatctggg gcggttttgt gctggtggcg cgggtgtaca
     5041 aacatatggc gaccctacgt gaaaccgaag acaagctgta cacggatttt caaactgtac
     5101 ttgaagggcg caaagagctg actctgaacc gggaacgcgc cgagtatgtg tttaacaacc
     5161 tctacatccc cgatgcgcaa gagtatcgcc atcatattat tcgcgcagac accttccatc
     5221 ttagtgccgt gaactggtca aacatcatga tgctgggcgc tatcggcctg gtgttctgga
     5281 tggcgaacag tctcggttgg gctgatacca acgttgctgc gacctattcg ttgacgctgt
     5341 tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt gccgacgctg ctgacggcgc
     5401 aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc tttcaaagct gagtttccgc
     5461 gcccgcaggc gtttcccaac tggcaaacgc tggagctgcg taacgtgacg tttgcttatc
     5521 aggataacgc gttttccgtt ggtccgatta atctcaccat caaacgtggc gagctgctgt
     5581 ttctgattgg cggcaacggt agcggaaaat cgacgctggc gatgttgttg acgggcttgt
     5641 atcagccaca aagcggcgaa atcttgctgg atggcaaacc tgtcagcggc gaacaaccgg
     5701 aagattatcg caaactgttt tcggcagtgt ttaccgatgt ctggctgttt gatcaactgc
     5761 tggggccgga ggggcaaccc gctaacccgc aactggttga gaagtggctg gcgcagctga
     5821 aaatggctca taagcttgag ttaagcaacg ggcgtattgt taacctgaag ttatcaaaag
     5881 ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga agaacgcgat attatcctgc
     5941 tggatgaatg ggcggcggat caggatccac acttccgtcg tgagttttat caggtgttgc
     6001 tgccgctgat gcaggagatg ggtaaaacta ttttcgctat cagtcatgat gatcattact
     6061 ttatccacgc cgaccgcctg ctggaaatgc gcaatgggca acttagcgag ctgacgggcg
     6121 aagagcgcga tgccgcttcg cgtgatgccg ttgcccggac ggcataatat tacgacgctg
     6181 acctggcgga ctgctgccgt caggtcaata tgctaaaaat cccttcattt ctaataaccc
     6241 tataattaat caacgaaatt ataatgtttc taaaattaga atataattta taaacattat
     6301 ttaaatgttg ttacttaagt gttaccgttg atgccgcgca aatctcacct gcaataaagc
     6361 gactaaaagt aaggcattaa caagatgaaa aaagtgactg ccatgctctt ctcgatggcc
     6421 gtggggctta atgccgtttc gatggcggca aaagcgaaag cgtccgagga gcaggaaact
     6481 gatgtactgt tgattggcgg cggcattatg agcgccacgt tggggaccta tttacgcgag
     6541 ctggagcctg aatggtcgat gaccatggtg gagcgcctgg aggggggcgc gcaggagagt
     6601 tcgaacggct ggaacaacgc cggaaccggg cattctgcac tgatggaact gaactacacc
     6661 ccgcaaaacg ccgatggcag catcagtatt gaaaaagcag tcgccattaa cgaagcattt
     6721 cagatttccc gccagttctg ggcgcaccag gtcgagcgcg gcgtgctgcg tactccgcgt
     6781 tcatttatca ataccgttcc gcatatgagc tttgtctggg gcgaggataa cgttaatttc
     6841 ctgcgcgccc gttacgccgc gttgcaacaa agctcgctgt ttcgcggtat gcgttactct
     6901 gaagatcacg cgcagatcaa agagtgggca ccgttagtga tggaagggcg cgatccgcaa
     6961 cagaaagtag cggcaacacg cacagaaatt ggcactgatg tgaactatgg tgagatcacc
     7021 cgccagttaa ttgcttcctt acagaagaaa tctaacttct cgctgcaact cagcagcgaa
     7081 gtccgcgccc taaagcgtaa tgacgataac acctggaccg ttaccgttgc cgatctgaaa
     7141 aacggcactg cacagaacat ccgcgccaaa tttgtcttta tcggcgcggg cggtgcggcg
     7201 ctgaagctgt tacaggaatc ggggattccg gaagcgaaag actacgccgg tttcccggtg
     7261 ggcggacagt tccttgtttc ggaaaatcca gacgtggtta atcgccatct ggcgaaagtt
     7321 tacggtaaag catccgttgg cgcaccaccg atgtcggttc cgcatatcga tacccgtgtt
     7381 ctggacggta aacgcgtagt gctgtttggg ccatttgcca ccttctcaac caaattcctc
     7441 aaaaacggtg cattgtggga tctgatgagt tccaccacca cctctaacgt gatgccgatg
     7501 atgcacgtcg ggctggataa tttcgatctg gtgaaatatc tggtgagtca ggtgatgttg
     7561 agtgaagagg atcgttttga agcgttgaaa gagtactatc cgcaagcgaa aaaagaggac
     7621 tggcgtttgt ggcaagcggg gcagcgtgtg cagattatca agcgtgatgc cgagaaaggt
     7681 ggcgtactgc gtctgggtac cgaagtggtc agtgaccagc aagggaccat tgcggcgctg
     7741 ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta tgttgaatct gctggaaaaa
     7801 gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta cgttgaaagc gatcgttccg
     7861 tcttatggac gcaagctgaa cggtgatgta gcggcaacag aacgcgagtt gcagtacacc
     7921 agcgaagtgc tggggctgaa atacgacaaa ccgcaagcag cagatagtac gccgaaaccg
     7981 cagttgaaac cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgta atgatgtgcc
     8041 acatccggca tggtatgccg gatgtggcgt atactgataa gacgcgccag cgtcgcatca
     8101 ggcaaccggc tcgggcgtta gatgttggat gcggcgtaac cgccttatcc aaccaacggg
     8161 gtgccgtact tcacaacagt ttagcgaact tccgcgttgt caattttctc ttccgccttc
     8221 cagacgcggt atttcacatc tacattgtct ggcgtataca ccacgatcgg cagcttgctg
     8281 ttgtaacgca gcattccagc atcgcccaga tacgcggtga caaatttctt ctctttcttg
     8341 ccatccgggc aggccatcat cgtggaaacc ggcgagctaa ctttatcaaa gacataataa
     8401 tcatagcccc agccttccag cgttttgctt tccagcttcc cgccgagacg atgcaaattg
     8461 caatcgactt ccagcgtctg accgattaac agttctactt tcagggtaga ttcatcttct
     8521 tgcggggtta actgaatcac ctgacgcttc atgcctgttt cagcttgtgg ataaggcgcg
     8581 attttttcca gtggctggac gctttctgcc gcccaggcgg aagtggtagc gaaagcggca
     8641 aacaatactg caggtagaat ggtcttcatt tttttgcttt ccttcttcat ttataggttt
     8701 tcaccgatga tgtcgctgaa ggttaacata ttcatcagat ttattaattt gctttatgaa
     8761 taacttaaag aacattcaga caattttacc tgctctttta gtgttaaatt ctgatgaggg
     8821 agcaaaccag cagaaatatc tctggtgaat ataatcgcta tataaatatg tttataacca
     8881 tttgaaatgt gagcaaaagc ccgtttttgc cacgcttccc gccactcttt tgatcctgct
     8941 acaggtttta ccccgatcgg ggtatgcatc tttgacacat cctttaatat cttagcggct
     9001 ataaaaatgg cttattaatt atgcggtcta tttggtcgct ctcaattttc agagcgcgtt
     9061 aatgatggaa ggtcaatgtg aagattgatg catcccgtcg gggcatactc actggtcgct
     9121 ggcgcaaagc cagtaacggt atccgtccgc cctggtccgg tgatgaatct cattttctga
     9181 cccattgcac ccgctgcgac gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg
     9241 gcacgggtgg ctatccgagc gttaatttca aaaataacga gtgcagcttc tgttacgcct
     9301 gcgcgcaggc ttgccccgaa tcgctatttt ctccgcgcca caccagggcc tgggatttgc
     9361 agttcaccat cggggacgcg tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg
     9421 atagctgtga gccaatggca attatctttc gcccgacgct ctccgggatc taccagccgc
     9481 aacttaatag ccaactttgc aacggatgtg gagcatgtgc agccagctgt ccggtatcag
     9541 ccataactgc ggagtatctc catgcacact aactggcaag tttgcagcct ggtcgtgcag
     9601 gccaaaagcg aacgaatttc agacatcagc acccaacaga acgcctttcc cggctgtgaa
     9661 gttgctgtca gcgacgcgcc gagcggtcag ttgattgtgg tggtggaagc agaagacagc
     9721 gaaacgctga tccaaaccat tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg
     9781 ctggtttatc accagcagga agagcaaggt gaggaaacac catgaaactc agtcgtcgta
     9841 gctttatgaa agc
//
LOCUS       DADLSB010000138         9819 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.138, whole genome shotgun sequence.
ACCESSION   DADLSB010000138 DADLSB010000000
VERSION     DADLSB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.138"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..896)
                     /locus_tag="JN331_003216"
     CDS             complement(<1..896)
                     /locus_tag="JN331_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7261286.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGG"
     gene            1137..1937
                     /locus_tag="JN331_003217"
     CDS             1137..1937
                     /locus_tag="JN331_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7261287.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2015..2785
                     /locus_tag="JN331_003218"
     CDS             2015..2785
                     /locus_tag="JN331_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7261288.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYFAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2833..4191)
                     /gene="mltD"
                     /locus_tag="JN331_003219"
     CDS             complement(2833..4191)
                     /gene="mltD"
                     /locus_tag="JN331_003219"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY7261289.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4263..5018)
                     /gene="gloB"
                     /locus_tag="JN331_003220"
     CDS             complement(4263..5018)
                     /gene="gloB"
                     /locus_tag="JN331_003220"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY7261290.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5052..5774
                     /locus_tag="JN331_003221"
     CDS             5052..5774
                     /locus_tag="JN331_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7261291.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5771..6238)
                     /gene="rnhA"
                     /locus_tag="JN331_003222"
     CDS             complement(5771..6238)
                     /gene="rnhA"
                     /locus_tag="JN331_003222"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY7261292.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWLHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6303..7034
                     /gene="dnaQ"
                     /locus_tag="JN331_003223"
     CDS             6303..7034
                     /gene="dnaQ"
                     /locus_tag="JN331_003223"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY7261293.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7167..7243
                     /locus_tag="JN331_003224"
     tRNA            7167..7243
                     /locus_tag="JN331_003224"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7201..7203,aa:Asp,seq:gtc)
     gene            complement(7374..8420)
                     /locus_tag="JN331_003225"
     CDS             complement(7374..8420)
                     /locus_tag="JN331_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY7261294.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFTRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8431..9306)
                     /gene="tssG"
                     /locus_tag="JN331_003226"
     CDS             complement(8431..9306)
                     /gene="tssG"
                     /locus_tag="JN331_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAY7261295.1"
                     /translation="MEKFRLGQTPALTFAPREIASVGWQEGRLHISLYSLGLWGPNGP
                     LPLHYTELARNRTESRRDPTLTRFSDLFHHRWLTQFYQAWRSAQSAGGGLDKPQHDRF
                     AFYVASLSGQDLRESADSPLPDHARLAASAHLVRESRNPDGLATTLAHYFGVPFRIQE
                     YVLHWIAVADDEITRLGMPAPSSVIGNGALIGQAVPDMQYKFRLVIGPLTLEDYRRFL
                     PGSNNLPVLTELVRAFSGYEYCWEIELQLKPHAAPPAVTGGPYQLGCRAWAGKAMHDN
                     PVTGMIFEPEHYLAH"
     gene            complement(9423..>9819)
                     /locus_tag="JN331_003227"
     CDS             complement(9423..>9819)
                     /locus_tag="JN331_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY7261296.1"
                     /translation="IRQLSFNYLPLADLDHRTGGQALRDLLNLFIPAHDSPQSRQVRS
                     LIGCKTTPVTRRLPGSGLLVYGRGVSCELTVDEEGFSGISPYLFGLVLEHYIARHVSI
                     NTFSQMTLHSMQRGKIMTWPVRAGQRGSV"
BASE COUNT         2318 a         2534 c         2510 g         2457 t
ORIGIN      
        1 cctccgggag ctgtttttac atgctcgcta aggaaatcga taaaagcccg gatgcgcgta
       61 cttaccgcac ggtcgctgta atagacggca ctgaatggca tttccactgg caacacttta
      121 tctgccatta actccaccaa ttctccgcga gcgatttctt tgtcgatcat gtagtcggac
      181 aaacacgcaa tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat
      241 gacaaaccgt acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg
      301 agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct
      361 ggcttcccgt agcgggaaat ataatcgggg gaggcgataa tttttcgata actgttaaat
      421 aacggcctgg cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc gacatccact
      481 tttctttcga tcaaattaat aatcgtttcg gaggagacta gcgataaagt gacttccgga
      541 tagcgttcac ggaaaggctt aattaacggc atcagaaagt gcagcaccac tggagttgcg
      601 gcatcgatcc gtaacagtcc acgcggtgta ttacgcgtct ccataatttc tgattctgcc
      661 gctgccatct cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc ttcttccgtc
      721 aggctaagtt gccgcgtggt ccgattaagc aggctaacgc caagtttcat ttccagcttt
      781 ttcaccgccc ggcttaccgc tgagtttgct tgccctaatt gttccgctgc ccggctaaag
      841 ctgccgcttt cgacgaccga aacaaaaatg gcgagttctt ccgacgtggc tttcattttt
      901 gctcctgttg caaaaaagat gagatatttt gaatttattt gtcattaaac catcaagatg
      961 cgtgatatgt catccgattt aatgttctca ataatgagca aatttctgac cggagtaagc
     1021 acttgcttac ataacaatat acaattgctc gttgaaagag tgagctaaaa tccctataac
     1081 agtagaaccc tcccgagtgc ggaagggttg acgtaataga ggtttcaaag tcaaaagtgc
     1141 gaaaaaacac ctatgccatg cgctatgttg ccggacaacc tgcggaaagg atcttaccgc
     1201 cggggtcttt tgcgagcatc ggccaagcat taccacctgg agaaccgtta agtaccgaag
     1261 agcgtattcg gatcctggtg tggaacatat acaaacagca acgcgctgaa tggttgtcgg
     1321 tattaaagaa ctacggcaaa gatgcacatc tggtgttatt gcaggaagcg cagacaacgc
     1381 cagagttagt acagtttgcg accgctaact atcttgccgc cgatcaggtg cccgctttcg
     1441 tgctgccaca acatccttcc ggtgtaatga ccctttcggc ggcgcatcca gtgtattgct
     1501 gcccgttacg tgaacgagaa cccattttgc gtctggcgaa gtcggcactg gtgacggtct
     1561 atccattacc tgacacccgc ctgttgatgg tggttaatat acacgccgtc aacttcagtc
     1621 tgggcgtgga tgtctatagt aagcagttac ttcctattgg tgatcagata gctcaccaca
     1681 gcggcccggt cattatggcg ggagatttca atgcctggag ccgtagaagg atgaacgcgt
     1741 tatatcgctt tgcgcgggaa atgtcgctgc gccaggtgcg ttttaccgat gatcagcgcc
     1801 gtcgggcgtt tggccgcccg ctcgattttg ttttctaccg tggtctgaac gtcagcgaag
     1861 cttctgtatt ggttacgcgc gcttccgatc acaatccgct actcgttgaa ttcagtcccg
     1921 gcaagcctga taaataaggt atgtcaggtc tgccacaggg cagaccaacg tttggcgctg
     1981 tccttttata ctcaaacaac aaaaggacag cgcaatgaca acacaatccc accatgacca
     2041 cgtagaaaag cagtttagct ctcaagcctg cgaatatctg accagtaccg tacatgcatc
     2101 cggacgagat ttacagcggc tggcggtgcg tctggctgac tatcctggtg caagtgtact
     2161 tgatatgggc tgcggagcag ggcatgccag ctttgttgct gcgcaaaacg tgagcgcggt
     2221 ggtggcgtat gacttatctg cccaaatgct ggatgtcgtg gcacaagctg ccgaagcccg
     2281 gcaactgaaa aatatcacca cccgccaggg atatgccgaa agtctgccat ttgccgataa
     2341 cgcatttgat attgttatca gccgttattt tgcccatcac tggcatgatg ttggtgcagc
     2401 actgcgtgaa gtgaaccgga tattgaaacc tggcggtagg atgattgtga tggacgtaat
     2461 gtctccgggt catccagtgc gcgacatctg gttacagacg gtagaagcat tacgcgatac
     2521 ctctcacgta cgaaactacg ccagcggtga gtggttgagg ttaatcaatg aagccaatct
     2581 gatagttgat aatttaatta cagataagtt accgctggaa ttttgttcat gggtcgcgag
     2641 aatgcgtacg ccagaagcgt tagtagacgc tattcgcatt taccaacaga gcgcatcgac
     2701 agaggtgaga acgtattttg ccttgcagaa tgacggctct ttcaccagtg atatcatcat
     2761 ggtagaagca cataaagcgg cataaataaa aaaggcaccg ggggaatcgg taccttttta
     2821 ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg tcagcttgtc
     2881 gcctggttgc agattcgcag tgtcgctgtt ccagcgcatc acatctttga tgttcacgcc
     2941 gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg taatgctatc
     3001 gctgttgttt gccagtcgct gtgcgctact gcctgcacca atcgtcaaac tttggcctgg
     3061 cttcagctta gatccgcgca gtttgttcca ctgctgcaaa tctttggtgc ttacgccgag
     3121 acgtgaagcg atgcttgaaa gcgtgtcgcc agagcgtacg gtgtaaacac ggctgttaag
     3181 cggcgtattg tcggcaacca gcgtcgactg tacagcagca atttcgcctg aagcgagaga
     3241 ttcacgcagt tgatctgcat gcttctttgg caccatcacg tactgcggcc cacttgcgcc
     3301 cagcgtggag cctttcacgc cagcgttgaa tgtcttcagc ttgctgacgg aaatccccgc
     3361 catatctgca accttcgcca tttcaaccgg gctactcagg tgcacacgcg ccagagcacg
     3421 gctttcatcg gtcgttggca gacgtacgcc ataacgcttg ctgtttttga gaatatcact
     3481 caatgccagc attttaggca cgtactgctt cgtttcctgc ggcaacggta acgaccagaa
     3541 gtccgtggat ttcccacgcg ctttgttcgt tttaattgcc ttcatgaccc gaccttcgcc
     3601 gctgttataa gccgctacgg tcagaagcca gtcgccgtca aacattttgt tcagacgctg
     3661 catcatattc agcgcggcag ttgttgaagc aacaacatcg cgacgcgcgt cataattgcg
     3721 ggtctgtttc aaaccataat tgcgccccgt gctcggaatg atctgccaga tgcctgcggc
     3781 attggcgcca gacgttgcgt gaggatcaaa agcgctctcc actatgggta gtagtaccag
     3841 ttccataggc atgttacgtt ttttaacttg ccctgctatc cagtacatat acggctctgc
     3901 ccgtaaagtt acatcgtgga gatagctctt attgcgtaaa tatttctgtt tctgttcgcg
     3961 aatccggtca ttttccggaa ttcccatctt tagctcgtcg ccaatgaaag cccacaagtc
     4021 accatctggc gcgatagacg tcccatcgtc catccatcgt gcctgacttg taaactttgc
     4081 tgcttcccct tgaccagctg cagaaaggct ctgtgcgtgc tgttgaacgt tgccggtact
     4141 ctggcaaccc acgagcagga cagaggcgag taatatcgct tttgccttca tgtgtgtgtc
     4201 aatagttgct taaaagacga ccgatcataa cggcgaacgg agccgatgac aagaaagttt
     4261 tatcagaacc tatctttctt tgaccttaac catgcaaaac gctcttcagg ttgttgcaat
     4321 aatgtttctt cattaattac attaattaaa tcaatatctt ccgttcttaa aaaaacatta
     4381 atttgccgct catttttcag aattacgggt agtgttattt gattttttgc ccgtaactcc
     4441 ttaactttac gataataatc atttatggac aaatcgtgcg gaaaaatact caaagcaaac
     4501 ttcatatttg ataacgtata ttcatgagca caacatacca atgtatcgtc aggtaacgca
     4561 cttagctttt taagtgattg atacatttgt aaagctgtcc cttcaaacaa ccgaccacac
     4621 ccaccagaaa acagcgtgtc gccgcaaaat agataaggtt tactgaagta acagatatgt
     4681 cctaaagtgt gacccggtgt ggtaattaca ctaaattcat gccctaaaac gaaggcagtt
     4741 tcgccatctt tgactacctg tgttgttccc ttatcttgtg tctcttgtgg accatacacc
     4801 acaatttgcg gaaacttttc caccagttct tttacgccgc caacgtgatc gtggtgatgg
     4861 tgggttagaa atatggcctc cggttgccag ttattggcgg taatggcgtt taatactggc
     4921 tctgcgtctc cgggatcgac aatcaggcag cgacctgcct catcattcaa aacccagatg
     4981 taattgtcat caaaggcggg aatactgtta agattcatag attacctctc agtgtaaaac
     5041 ggaaggttgt gatgaaaccg gcaagagtcc ctcaaactgt cgtggctcct gattgctggg
     5101 gcgatttgcc ctggggagag ctttatcgca aggcgctgga gcgccagctc aacccgtggt
     5161 tcactaaaat gtatggtttt catctgctta agattggcaa tttaagcgca gaaatcaatt
     5221 gcgaagcgtg cgcggtttct catcaagtga atgtttctgc gcaaggaatg cccgtccagg
     5281 tgcaggcgga tccacttcat cttccttttg ccgataaatc cgttgatgtt tgtctactgg
     5341 cacatacctt gccgtggtgc accgatccgc atcgtttatt acgtgaagcc gatcgggtac
     5401 tgattgatga tggctggctg gtgattagtg gcttcaaccc catcagttta atgggattac
     5461 gcaaacttgt gccggtattg cgcaaaacct cgccctataa tagccggatg tttactctga
     5521 tgcggcaact ggactggctc tctttgttga attttgaagt gctgcacgcc agccgtttcc
     5581 acgttctccc gtggaacaaa cacggaggaa aactattgaa tgcgcatatt cctgcgcttg
     5641 gttgcttaca acttattgtt gcccggaaac ggactattcc tttaacgcta aatccgatga
     5701 aacagagtaa aaacaagcca cgaattcgcc aggcggttgg agccacccgg caatgtcgta
     5761 aaccacaggc ttaaacttcg acttggtagc ccgtatcttc cagtgtggga ttcatcgccg
     5821 cggcacgagc cagttcatca cagcgttcgt tttccgggtg tccggcatgg cctttaaccc
     5881 attcccattt gatttgatgc tgccccaatg cagcatcaag acgttgccag agatcgacat
     5941 tttttactgg ttttttgtct gctgttttcc agccacgttt tttccagtta tggagccact
     6001 gggtgatacc ctggcggaca tactggctgt cggtactcaa aatgacttcg caatgttctt
     6061 ttaacgcctc cagcgcgaca atagcggcca tcaactccat acggttgttg gtggtgcggg
     6121 tgtagcctgc gctaaaggtt ttctcgcgtc cgcgatagcg taaaatagcg ccgtaacccc
     6181 caggtccagg attacccaga cacgaaccat cggtgaaaat ttctacctgt ttaagcatct
     6241 ctggtagact tcctgtaatt gaatcgaact gtaaaacgac aagtctgaca taaatgaccg
     6301 ctatgagcac tgcaattaca cgccagatcg ttctcgatac cgaaaccacc ggtatgaacc
     6361 agattggtgc acactatgaa ggccacaaga tcattgagat tggtgctgtt gaagtagtga
     6421 accgtcgcct gacgggcaat aacttccatg tttatctcaa acccgatcgg ctggtggatc
     6481 cggaagcctt tggcgtacat ggtattgctg atgaattttt gctcgataag cccacgtttg
     6541 ccgaagtagc cgatgagttt atggactata tccgcggcgc ggagttggtg atccataacg
     6601 cagcgttcga tataggcttt atggactacg agttttcgtt gcttaagcgc gatattccga
     6661 agaccaatac cttctgtaag gtcaccgata gccttgcggt ggcgaggaaa atgtttcccg
     6721 gtaaacgcaa cagcctcgat gcgctatgtg cccgctacga aatagataac agtaaacgaa
     6781 cgctgcacgg ggcattactc gatgcccaga tccttgcgga agtttatctg gcgatgaccg
     6841 gtggtcaaac gtcgatggct tttgcgatgg aaggagagac acagcagcaa caaggtgaag
     6901 caacaattca gcgcattgta cgtcaggcaa gtaagttacg cgttgttttt gcgacagatg
     6961 aagagctggc agcacatgaa gcccgtctcg atctggtgca gaagaaaggc ggaagctgcc
     7021 tctggcgggc ataaatacat gtaaaaggcg ctaaaaatag cgacttgggc gatttttgca
     7081 gcaaacgatt caaaagatga gaaaaatcgt tgacgaaggt cgaggcaatc cgtaatattc
     7141 gcctcgttcc caacggaaca caacgcggag cggtagttca gtcggttaga atacctgcct
     7201 gtcacgcagg gggtcgcggg ttcgagtccc gtccgttccg ccaatattca gaaagcccga
     7261 atcagtaatg attcgggctt tcgtcgtttt ggtgaagtga gaatttttca ctccgaagta
     7321 aaacggatat gatttaaaaa atggccagac attataaatt ctggccactt ctgttactcc
     7381 agcgcatccc atttctccag cagttctact ggaatttcat ttattacctc cgagacgcgt
     7441 tttccgacca ggcgttctgc ctggcgcagc aaaggaatgg tcgggctgct cggttcgctg
     7501 tgctcaaacc agcgacggat gacacgcagg cgttccagcg cgtcgtttcg gtcgcggatc
     7561 tgccggggtt ctgcgccggt catcggtgat atttcctccg cgatatgcac cggtagcgca
     7621 atctgctctg cctgcatctg ttcagtatga acgatggtgg cgttttccgg ctgctcagcc
     7681 tgtggttgtg gcaggatctc cggcgtggtg ctctgcactg ctcccggtaa cagggccagc
     7741 agttgcgtca gacgggtgaa gtccggtgcc tggtcattca gcgtttcacg cgcatggcgt
     7801 tgcagccgct cagtggtctc tgctgcctgg cgaaaggcat ccagcggcag tgtgccgcgc
     7861 gcttccaggt ctgccagttg ctggcgtacc gattctggtg ccagcgcatc cgccgggcgt
     7921 aatgcagata gcgaacgctc cacatcccgc acctgaaggc gcatggcagc attatttgac
     7981 agtgtaatgc cacgaatatc cgccattaca ccttcgtggt ccagcaacgc cgccagcgcg
     8041 ttactgcgtg ccaccgcagc atcttcggcg gtgatatctt cagtcgcgag cagttgagga
     8101 tggatggcgt ccgggtacgt ggagcacaac gtttccagca gcgtgagcgc ctccgacagt
     8161 cccctggcac cggcctgctg gatacggcag cgcagcagga caaccagaat gcggatatct
     8221 ttgctgcgca tcagcagacg atgggcgtcc cgctcaagct ccgcccagtt aatgttttcc
     8281 ggcgtactga caaaatcgcc atattgcgct tcttcacgcg gtgcggcgcg ggtgaacagc
     8341 agcaggtatt ccggatcgta ctccagatcc gggccgcagg ggcattccgc cgaaattggg
     8401 tcagacagga atatttccat ttaattatcc tcagtgcgcc aggtagtgtt ctggttcgaa
     8461 aatcatgccc gtcaccgggt tgtcgtgcat ggctttaccc gcccatgcac gacaacccag
     8521 ttggtacgga ccaccggtaa cagccggagg ggcggcgtgg ggctttagct gtagctcaat
     8581 ttcccagcag tattcataac cactaaaggc gcggaccagt tctgtcagga cgggcaggtt
     8641 attgctgcca ggcagaaaac gtcggtaatc ctccagcgtc agcgggccga taaccagccg
     8701 gaacttatac tgcatgtccg gcacggcctg gccgatgagt gcaccattgc caataacgga
     8761 agagggggca ggcatcccca gccgggtgat ctcatcgtcc gccacggcta tccagtgcag
     8821 gacatactcc tgtatccgga aaggtacacc gaaatagtgc gccagcgtgg tggccagacc
     8881 gtccggattg cgggactcac gcaccagatg ggccgatgcc gccagacgag cgtgatctgg
     8941 caacgggctg tcagcgctct cccgcaaatc ctgcccgctg aggctggcga cataaaaggc
     9001 aaagcggtca tgttgcggtt tatccagacc gcccccagcg gactgggcgc tgcgccatgc
     9061 ctggtaaaac tgcgtcagcc agcggtgatg aaaaagatca gaaaaacggg tcagcgtcgg
     9121 atcgcgtcgg ctttcagtcc ggttacgcgc cagttcggtg tagtgcagcg gcagtggacc
     9181 gttcggcccc cacagcccga ggctgtacag actgatgtgc aggcgccctt cctgccagcc
     9241 cacactggcg atttctcgtg gcgcaaaggt cagcgcgggc gtttgtccga ggcgaaattt
     9301 ttccatccgt ggttgctagg tgttgcccgc cggaacggta cacaactgca catccacgcg
     9361 gcgcatcagg ttcagaaaac cgtaacgcca ggggggttcc tgcgcctgag tgaaatcgtt
     9421 gctcatacgc ttccccgctg tcccgccctg acaggccagg tcataatttt gccgcgctgc
     9481 atgctgtgca gcgtcatctg cgagaaggta ttaatggaaa catggcgggc gatatagtgt
     9541 tccagcacca ggccgaacag atacggactg atgccggaaa aaccttcttc atccaccgtc
     9601 agttcacaac tgacgccacg cccatagacc agtagtccgg aaccgggcag acggcgggtg
     9661 accggggtcg ttttacaacc aatcaggctg cgcacctggc gcgattgcgg actgtcatga
     9721 gcaggaataa acaggttcag cagatctcgc aacgcctggc cgccggtacg gtgatccaga
     9781 tcggccagcg gcagataatt aaaagataac tggcggatc
//
LOCUS       DADLSB010000139         9816 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.139, whole genome shotgun sequence.
ACCESSION   DADLSB010000139 DADLSB010000000
VERSION     DADLSB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.139"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..338)
                     /locus_tag="JN331_003228"
     CDS             complement(<1..338)
                     /locus_tag="JN331_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY7261297.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDE"
     gene            complement(338..1045)
                     /locus_tag="JN331_003229"
     CDS             complement(338..1045)
                     /locus_tag="JN331_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7261298.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAISAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIANGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1060..1737)
                     /gene="ydjY"
                     /locus_tag="JN331_003230"
     CDS             complement(1060..1737)
                     /gene="ydjY"
                     /locus_tag="JN331_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY7261299.1"
                     /translation="MSQHYSVSWEKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(1742..2452)
                     /locus_tag="JN331_003231"
     CDS             complement(1742..2452)
                     /locus_tag="JN331_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7261300.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(2619..3425)
                     /gene="xthA"
                     /locus_tag="JN331_003232"
     CDS             complement(2619..3425)
                     /gene="xthA"
                     /locus_tag="JN331_003232"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY7261301.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            3871..5091
                     /gene="astC"
                     /locus_tag="JN331_003233"
                     /pseudo
     CDS             3871..5091
                     /gene="astC"
                     /locus_tag="JN331_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5088..6122
                     /gene="astA"
                     /locus_tag="JN331_003234"
     CDS             5088..6122
                     /gene="astA"
                     /locus_tag="JN331_003234"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY7261302.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            6119..7597
                     /gene="astD"
                     /locus_tag="JN331_003235"
     CDS             6119..7597
                     /gene="astD"
                     /locus_tag="JN331_003235"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAY7261303.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            7594..8937
                     /gene="astB"
                     /locus_tag="JN331_003236"
     CDS             7594..8937
                     /gene="astB"
                     /locus_tag="JN331_003236"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY7261304.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            8930..>9816
                     /gene="astE"
                     /locus_tag="JN331_003237"
     CDS             8930..>9816
                     /gene="astE"
                     /locus_tag="JN331_003237"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY7261305.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVT"
BASE COUNT         2260 a         2490 c         2764 g         2302 t
ORIGIN      
        1 tcttcatcgc taaagagcgg gctttgccgc cagtcagcca ctgccagcag tttatcggtg
       61 ctgccggtac gctcggcgac tttcatactg gtgatatcca cgcaaaactc acacaggcac
      121 atttgcgcaa tgcgcgcgct gacaagcgat cgcactaccg gatcgagcgg tgagcgtttg
      181 cgctccagcc acccaacaaa cattgacacc aaataaaaga taaacgggat ccgcccccac
      241 cagcgaatcg ggcttagcac ctcgccgtaa tggcgacgat gcagccacgc ctgcggacgt
      301 aaaatgaaag gaattttgct aagcggcggt aaacccatca gtcattcctc ttctggcgct
      361 caagccatat tttcttcgcc ataaaaatca ccacggtcag ggcaaacaga ataaacagtc
      421 cggtgacaaa ccagaaagta ccgcctgtta acatgctgcc cgcccaggaa taaacaatag
      481 tcgccggtaa ttgaccaagc ccgttggcga taaaaaacga gcgaaaacgt attgaagtca
      541 gaccggcagc atagctgatt ggatcgaaag ggacaaaagg caataaccga cagaccagga
      601 tggtgtgttt gccgtagcga gtgaaaaagc cgtccatact gtcaagcacg gttttgccgg
      661 ttaatttttc caccacttcg cggcccatca ctctggcgat aaaaaagcac agcgccgcgc
      721 cggccatcga actggtccac gacagcaagc cgccccagaa cgcgccaaac agcgacgcat
      781 tggcaaaggt gatcaaaaac gcaggtagcg gtgcagaaat ggcctgtaaa atcatcaaca
      841 agaacgagac aactgccgcc agtgcgccgt aagactgaat aaagcgttct ataccctgtt
      901 ggtccacggc ggcaaacgcc gcaacgctgc gattgataaa ctcatggacg ccgggaagca
      961 gcgcccatgc gagcagcata gcgaacaaca ggatgatgag ggttatacgg tagtaccaga
     1021 ttttacgcga ttgtatcttc atcatccttt atcctggctt tattcggtga ttttaaaggt
     1081 aaccgttgcc agcgtgttat ccgccgggag aactgaggca ttgcctttga atttcacttc
     1141 gccgcgtttt tccaccgcac cataagtgta tgttgcattg ctgacgatgc cgaccgggca
     1201 gctatccaga cacaccaggc aacccgtttt cttctcttct gctgccgtta aattaccgcc
     1261 aaagcgcatg tccagtttct tgccattact gtcaacaatc acttcatcga aggaatacgc
     1321 ttttgccgcc ccttgccagt tgaccgaaat atccagtttg ctgcctgtga catgagtcgt
     1381 ttctttatta tccatcgtca tgttttcgcc cggcgtacca cctgcctctt tcagggcttc
     1441 ataaaatgct ttcggggtcg cataagccat aaacagcgat ttatgtccgt tggagccatc
     1501 tttaaacaca ataccgtgac gagtgtcgtc ggtgaggaaa cgaccattaa tttgcaccag
     1561 catggttacc gtatggttat tggcatcgac gcgcagcggt tggctgttcg aaagtccgtc
     1621 gtattccact tttgccgccg cattctcttt ttgatcgcag ccgctaagcc cggcaacagc
     1681 cagtaagcag agtgcagcca gtcctttttc ccatgacact gaataatgtt gcgacatcgt
     1741 tctatccttc attttttggg tgagtaagtg ggctgcggcg cgaagcagac agatccacat
     1801 gtttgtgtcg ggcgtagagt tttgcgagct ggatgagaat aaacagcgcc aggcccgcca
     1861 gacagagttg taaaataaag cgcagcgtaa tgccttcatt ggcgagatcg cttgccatca
     1921 cggtataaat aacaataccg ggcagggtcg tgagtgccga aataagggta taaggccaga
     1981 aggcgatggt ggttaatccg taagcgtaat tttgaatatt gtaaggaaac aacgggatta
     2041 agcgggtcag aataagaaaa tcaataccgt tacgcgcaat gcctttttca atggcctgga
     2101 aggtatggct atggcccacg tatttcagca gtaaatcacg tcccagccag cgcgccagca
     2161 ggaatgagca cgaggaggcc agcgttgcgg caattaatga gagtagtgtc cctaagagcg
     2221 ggccaaaaac tattcctcca gcgatcacca gtatgcttcc tggtaacaga aagagggtgg
     2281 cattgatgaa taacagaata tagaggctat aaccgaacaa tccgctctgg cgaatgagtg
     2341 tctgtaagtg gggtaaatcg gtgagcagat cgaataaacc gaaagcgtgg atagcgtaaa
     2401 tgaccagcgc aaaaataaga caggcaaaca gaaagttacg ctcagcgttc atcgtggagt
     2461 ttcctatatc aaacttgata atagagaagt ggtcagacaa ctaaaaacgc agtgacggta
     2521 taacaaagga cggcaggcaa caaatcatta ctgacagttt gttgattttt ctcaaggtta
     2581 attctcctga cccagtttga gccaggagag ctgctaaatt agcggcggaa ggtcgcccag
     2641 acgggggcgt gatcggacgg tttttccatg ctgcgaattt catagtcgat gccggtttct
     2701 acgcaacatt ctgccagcgg ttggctggcg agcagcaggt cgatgcgcag accacggtta
     2761 tcgtcaaaac cttttgagcg gtaatcaaac catgagaaac gatctgctgt ttgcggatta
     2821 gcatggcgga aggtatcgac caccccccag ctcatcagcc tgtccatcca ttcgcgctct
     2881 tccggcagga aagagcattt accggtacgc agccagcgct tacggttttc ttcgccaatg
     2941 ccgatatcca gatctgtagg gctgatattc atatcgccca taatcagtac cggattatca
     3001 cgtttgagtt cggtttccag gtagttttgc agattctgat aaaactgcgc ttttgccggg
     3061 aattttatcg gatggtcgcg gctttcaccc tgcgggaagt aaccgttgat cacggtgaca
     3121 ttacccagca gtgaggggat ttccgccata ataatccgcc gctgcgcctc ttcgtcgtca
     3181 ccgggaaagc cgcgacgcac ggcaatcggc gtctctttgg tcagcagcgc cacgccataa
     3241 tggcctttct gcccgtgata aaacacgttg tagccgagct tcgccacctc ttcgagcgga
     3301 aacatatcgt catgaacttt tgtctcctgc aggccaatca catccggttg gtgcttttcg
     3361 acgatggctt caagctggtg aggtctggcg cgcaggccgt tgatattaaa agagacaaat
     3421 ttcatagtcg ctgccattta ttcagataaa gagtgcgtgg atggtagcag aatttcgcgt
     3481 tgcttaccgc ctgttgttaa gtgatttgat cgatggttgt taatgatgtc aacgatggcg
     3541 caaaaaatgc ccgcttttgg tgcgcgctgc gtcagaatgg cgcagtaatt tccagtaaat
     3601 ttcgtcaatg atcacaattc cagaattata ttttacttct gcataacatt gcgtttttta
     3661 ttctttttat tcacccacat cgcaaacgta ttcactttat atgcacttta aatgcatatg
     3721 gtttggttat aactccttga tttccagtta gcctccgccg tttatgcact tttatcactg
     3781 gctggcacga accctgcaat ctacatttac agcgcaaaca ttacttattg ttaacataca
     3841 aataacgaat tatttactgt agaggtcgct atgtctcagc caattacgcg tgaaaacttt
     3901 gatgaatgga tgatacctgt ttacgctccg gcacccttta taccggtacg tggcgaaggt
     3961 tcgcgcttgt gggatcagca ggggaaagag tatatcgact tcgcgggtgg cattgcggtg
     4021 aacgcgctgg gccatgcgca tccggaactg cgtgaagcgc tgaacgaaca ggcgagtaag
     4081 ttctggcata ccggcaacgg ttacaccaac gagccggtac tgcgactggc gaaaaaattg
     4141 atcgacgcca cgtttgccga tcgcgtcttc ttttgtaact ccggtgcgga agccaacgaa
     4201 gcggcgctaa aactggcgcg taaattcgct cacgaccgct acggcagcca taagagtggc
     4261 atcgtggcgt tcaaaaatgc gtttcatggt cgcacgctgt ttactgtcag tgcgggtggg
     4321 cagccagcct attcacagga ttttgcgcca ctgccggcgg atattcgtca tgctgcatat
     4381 aacgatatta actctgccag cgcgctgatt gacgacgcca cctgtgcggt gattgtcgag
     4441 cccatctagg gcgaaggcgg tgtggtgcca gccagcaacg cgtttttaca aggtctgcgt
     4501 gaattgtgtg accgccacaa tgccttgctg atttttgatg aggtacaaac cggcgtcggg
     4561 cgcactggtg agctgtatgc ctatatgcac tacggcgtga cgcccgatct gttaaccacc
     4621 gccaaagcgc tgggcggcgg tttcccggtc ggtgcgttgt tggcaaccga agagtgcgcc
     4681 cgcgtgatga ccgttggcac tcatggcacc acctatggcg gtaatccgct ggcctcggcg
     4741 gtggcaggca aagtgctgga gctcatcaac acaccagaga tgcttaatgg cgttaaacag
     4801 cgtcacgact ggtttgttga gcgtcttaat actattaatc accgctatgg tttgttcagt
     4861 gaagttcgcg gcttaggttt gttgattggc tgtgtactga atgccgatta cgccgggcaa
     4921 gcgaaacaga tctctctgga agcggcgaaa gcaggcgtga tggtactgat tgcgggtggc
     4981 aacgtggtgc gttttgcgcc tgcgctcaat gtcagcgaag aagaggtgac gaccggactg
     5041 gatcgctttg cagctgcttg cgaacacttt gttagccgag gttcatcatg atggtcatcc
     5101 gtcccgttga gcgctcagat gtctcggcgc tgatgcagct tgccagcaaa acgggcggcg
     5161 gcctgacgtc gcttcccgcc aatgaagcca cgctttcggc gcgtatcgaa agggcaatca
     5221 aaacctggca aggcgaactg cccaaaagcg agcagggcta tgtgttcgtg ctggaagata
     5281 gcgagacagg caccgtggcg gggatttgcg ctattgaggt ggcggttggg ctgaacgatc
     5341 cctggtacaa ctatcgcgtc ggcacgttgg ttcacgcctc aaaagagctg aatgtctata
     5401 acgcattgcc gacgctgttt cttagtaacg atcacaccgg cagcagcgag ctgtgcacgc
     5461 tgtttctcga cccggactgg cgtaaagagg gcaacggcta tttgctgtcg aaatcgcgct
     5521 ttatgtttat ggcggctttt cgcgacaagt ttaatgacaa agtggttgct gaaatgcgcg
     5581 gggtgattga cgaacacggc tattcaccgt tctggcaaag cctcggtaaa cgcttctttt
     5641 cgatggattt tagccgcgcc gattttctct gcggcaccgg gcaaaaggca tttattgcag
     5701 aactgatgcc gaaacatccg atctataccc actttttatc ccaggaagcc caggacgtca
     5761 tcggtcaggt acatccgcaa accgcgcctg cccgcgcggt gctggagaaa gaaggttttc
     5821 gttaccgcaa ctatatcgac atcttcgacg gcgggccaac gcttgagtgt gacatcgacc
     5881 gcgtgcgcgc catccgtaaa agtcggctgg tggaagtggc agaagggcag cctgcgcagg
     5941 gcgattttcc ggcctgcctg gtcgccaatg aaaattatca ccatttccgc gtggtgctgg
     6001 cgcgtaccga tccggcaacc gagcgtttga ttttaaccgc cgcacaactg gatgccctca
     6061 aatgccacgc aggggatcgc gttcgtctgg tgcgcctgtg cgcagaggag aaaacagcat
     6121 gactttatgg attaacggtg actggataac gggccagggc gcatcgcgtg tgaagcgtaa
     6181 tccggtatcg ggcgaggtgt tatggcaagg caatgatgcc gatgccgctc aggtcgaaca
     6241 ggcttgtcgg gcagcccgtg cggcgtttcc gcgctgggcg cggctctcat tggctgaacg
     6301 tcaggtcgtt gtcgaacgct ttgccggatt gctggaaagc aataaagccg aattaaccgc
     6361 gattattgcc agagaaacgg gtaagccgcg ctgggaagcg gcaaccgaag tgacggcgat
     6421 gatcaataaa atcgcgatat caattaaggc gtatcacgtt cgtaccggcg agcagcgtag
     6481 tgaaatgccg gacggtgcgg cgagcctgcg acatcgcccg cacggcgtgc tggcggtgtt
     6541 tgggccgtat aatttccctg gtcatttgcc gaacggacat atcgttccgg cattgctggc
     6601 aggtaacacc attatcttta aacccagcga actgacaccg tggagtggcg aagcggtaat
     6661 gcgtttatgg cagcaggctg gcttgccgcc gggcgtgctg aacctggtgc agggcgggcg
     6721 tgaaacgggt caggcgctga gtgcgctgga ggatctcgac ggtttgctgt ttaccggtag
     6781 cgccaatacc ggctaccagc tgcatcgcca gctctccggt cagccggaga aaattctcgc
     6841 ccttgagatg ggcggtaata atccgctaat tatcgatgag gtggcggata tcgacgcggc
     6901 tgtccatctg accattcagt cggcgtttgt cacagccggg caacgctgca cctgcgcccg
     6961 ccgtttattg ctcaaaagcg gagcgcaggg cgatgcgttt cttgctcgtc tggttgccgt
     7021 cagccagcga ttaacgccgg gcaactggga tgacgaaccg cagccgttta ttggcgggct
     7081 gatttctgaa caggccgcac agcaggtggt tactgcctgg cagcaactgg aagcgatggg
     7141 cggacgaacc ctgcttgcgc cgcgcttatt acaagcaggg acatcgttgc tgacaccagg
     7201 gatcattgaa atgacgggcg ttggcggtgt gccggatgaa gaggtgttcg ggccgctttt
     7261 gcgcgtctgg cgttatgata cttttgatga ggcgattcgg atggcgaaca acactcgctt
     7321 cggactcttt tgcggtctgg tttcccccga gcgggaaaaa ttcgatcaac tgttgctgga
     7381 ggcgcgggcg gggattgtta actggaacaa accgcttact ggtgctgcca gtaccgcgcc
     7441 attcggcggc attggtgctt ccggtaacca tcgccccagc gcctggtatg ccgcagatta
     7501 ctgcgcatgg cctatggcga gcctggagtc ggactcgtta acattgcccg ccacgcttaa
     7561 ccccgggctg gatttttccg atgaggtggt gcgatgaacg cctgggaagt taatttcgac
     7621 gggctggtag ggctgacgca tcattacgcg ggcctgtcgt ttggtaatga agcctctacc
     7681 cgtcaccgtt ttcaggtgtc taacccgcga ctggcggcaa aacagggctt actgaaaatg
     7741 aaagcccttg ccgatgcggg attcccccag gccgtgatcc cgccgcacga gcgtccgttt
     7801 attccggtgc tgcgtcagtt gggattcagt ggtagcgatg agcaggtact ggaaaaagtt
     7861 gcacgccagg caccgcactg gctttccagc gtcagttccg cttcgccaat gtgggtagcc
     7921 aatgcggcaa cgatcgcgcc atctgccgat acgctggatg gcaaagtgca tctcaccgtt
     7981 gccaacctga acaataaatt tcaccgttcg ctggaagcgc ccgtcactga atcgctgtta
     8041 aaagcgattt ttaaggacga agagaaattt agcgtccatt cggcgttgcc gcaggtagcg
     8101 ttgctcggtg atgagggggc ggcaaaccac aatcgtctcg gcggtcatta cggtgaaccg
     8161 ggtatgctac tttttgtcta cgggcgagaa gaaggcaatg atacccggcc ttcccgttat
     8221 ccggcgcgac agactcgcga agccagcgag gcggtggcaa ggcttaatca ggtgaatccc
     8281 caacaggtga ttttcgccca gcaaaacccg gacgttatcg accagggcgt ttttcataat
     8341 gacgtgattg ccgtgagtaa ccgccaggtg ctgttttgcc accaacaggc gttcgctcgc
     8401 cagtcacagt tactggcaaa cctgcgggcg cgggtgaacg gttttatggc gatagaagtt
     8461 ccggcaactc aggtttccgt gtcggatgcg gtgtctacct atctgtttaa cagccaactg
     8521 ttgagccgcg atgatggttc catgatgttg gtgctgcctc aggagtgtcg ggaacacgcc
     8581 ggagtatggg gttatctcaa tgaactcctt gccgctgaca acccgattag cgaactaaaa
     8641 gtctttgact tacgtgaaag catggcgaat ggcggcggcc cggcgtgcct gcggttgcgg
     8701 gtggtattga cagaagaaga acgccgggcg gtgaatccgg cggtgatgat gaacgatacc
     8761 ttgtttaatg cgctcaatga ctgggtggat cgttactacc gcgatcgtct tactgctgcc
     8821 gatctggccg acccgcaatt gctgcgcgaa gggcgggaag cactggatgt attgagccaa
     8881 ttactgaatc tcggttcggt ttatccgttc cagcgcgagg gagggggcaa tggataattt
     8941 tcttgctctg accttaacgg gtaaaaaacc ggttatcacc gagcgagaaa tcaacggcgt
     9001 tcgctggcgc tggctgggcg atggtgtgct ggaactgacg ccattaacgc caccgcaagg
     9061 cgcgctggtg atttctgcgg ggatccacgg taatgagacg gcacctgtgg aaatgctgga
     9121 cgcgttgctt ggcgcgatat ctcacggcga gatcccgtta cgttggcggt tgctggtgat
     9181 cctcgggaat cctcctgcgc tgaagcaagg gaaacgttat tgccatagcg atatgaaccg
     9241 aatgtttggc ggtcgttggc agctatttgc tgaaagcgga gaaacctgtc gggcgcgcga
     9301 actggaacag tgcctggaag atttttatga ccagggcaaa gaatctgtgc gttggcacct
     9361 tgatctacat accgcaattc gtggctcctt gcatccgcag ttcggtgtat taccgcaacg
     9421 cgacattccc tgggacgaga aatttctgac gtggctgggt gcggcggggc tggaggcgct
     9481 ggtgttccat caggagcctg gtggtacgtt tacccatttc agcgtcagac attttggcgc
     9541 gctggcctgt acgttggaac ttggcaaagc gttgcccttt gggcaaaacg atcttcgcca
     9601 gtttgcagta actgccagcg caattgctgc gctgctatct ggtgagagtg tcggtatcgt
     9661 gagaacaccg ccgctccgtt atcgggtggt ttcgcaaatt actcgccact cgccgtcctt
     9721 cgaaatgcat atggcaagtg acacgctgaa ttttatgccg tttgagaaag gaacattgct
     9781 ggcgcaggac ggagaggaac gttttaccgt aaccca
//
LOCUS       DADLSB010000140         9796 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.140, whole genome shotgun sequence.
ACCESSION   DADLSB010000140 DADLSB010000000
VERSION     DADLSB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.140"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..335
                     /gene="tqsA"
                     /locus_tag="JN331_003238"
     CDS             <1..335
                     /gene="tqsA"
                     /locus_tag="JN331_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY7261306.1"
                     /translation="YIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFGNI
                     LEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSIAV
                     LLSDLNKE"
     gene            complement(360..1748)
                     /gene="pntB"
                     /locus_tag="JN331_003239"
     CDS             complement(360..1748)
                     /gene="pntB"
                     /locus_tag="JN331_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY7261307.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(1759..3291)
                     /gene="pntA"
                     /locus_tag="JN331_003240"
     CDS             complement(1759..3291)
                     /gene="pntA"
                     /locus_tag="JN331_003240"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY7261308.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQSQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            3815..4759
                     /locus_tag="JN331_003241"
     CDS             3815..4759
                     /locus_tag="JN331_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7261309.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            4945..6327
                     /locus_tag="JN331_003242"
     CDS             4945..6327
                     /locus_tag="JN331_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7261310.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVSVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            6364..7086
                     /gene="folM"
                     /locus_tag="JN331_003243"
     CDS             6364..7086
                     /gene="folM"
                     /locus_tag="JN331_003243"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY7261311.1"
                     /translation="MDKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTVPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(7083..7418)
                     /locus_tag="JN331_003244"
     CDS             complement(7083..7418)
                     /locus_tag="JN331_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY7261312.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            7547..8290
                     /gene="rstA"
                     /locus_tag="JN331_003245"
     CDS             7547..8290
                     /gene="rstA"
                     /locus_tag="JN331_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY7261313.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            8515..>9796
                     /locus_tag="JN331_003246"
     CDS             8515..>9796
                     /locus_tag="JN331_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HAY7261314.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGGGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSMMSGVISARLLKD"
BASE COUNT         2426 a         2476 c         2343 g         2551 t
ORIGIN      
        1 attacatccc taatattggt tcagttctcg cggcaatccc ccctatcgct caggtactgg
       61 tgtttaatgg cttctacgaa gcgttgctgg tgctggcggg atatctgctg attaatctgg
      121 tcttcggcaa tattctggag ccgcgcatca tgggacgtgg gctggggctt tcgacattgg
      181 tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt atgctgcttt
      241 ccgtgccgtt gacaattatt gtcaaaattg cgcttgaaca aacagcggga ggtcaaagca
      301 tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg ccgtcagggt
      361 tacagagctt tcaggattgc atccacgctg gctttggcgt caccaaacag catgtgggtg
      421 ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat cgaacgttta
      481 aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc aatcggactc
      541 ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac cagtacggta
      601 tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc atacggtact
      661 tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac cgggtggata
      721 ccgaaacgca cattaatacc acgagcgcgg agtttctcgg taatttcagc gacaggatat
      781 tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg ggagtttttc
      841 agcagttccg ctgtctcttc tgcggtgatt tcgcggtgct cacccacttc ctgatcatcg
      901 ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa ggaacggttc
      961 atcgccttac acataatgta agaaaggata gcccccgaag aaccgaccag cgcaccggtc
     1021 acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca gccggagtac
     1081 gagttcagca tcgacaccac caccggcata tctgcaccac cgatggaggc gactaaatgc
     1141 cagccgaata ccagcgcaat ggcggtcatt atcagcaatg ccagcacttg caggccgacg
     1201 ctgtccgtgc gaacaaatac aatcagcagc aagaaggaaa cgaccagagc cgccaggttc
     1261 attttgtgac ggtttggcag catcaacggt ttagacgaaa tcttgccaca cagtttgccg
     1321 aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag gaacacttcc
     1381 gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag ataactgtta
     1441 aagccaacca gcactgccgc cagacccacg aagctgtgca ggatcgccac cagttctggc
     1501 atttcggtca tttcaacttt cttcgccagc cggataccaa ttgccccacc aatgaccatc
     1561 gccagcaaga tccagccaac attacccgta tccggtccaa aaatggttgc gattaacgca
     1621 atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg tttcgaaaga
     1681 ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt aactaatcct
     1741 ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct gagtcacggt
     1801 gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa ggaagctaac
     1861 ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg aaatcgcgtt
     1921 ggtaaccgac atcaacggtg tatgcagcgc gtgcgataca ttccacacca cgtaataacc
     1981 gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg gcgcaacgct
     2041 tgacagccaa ccgaacagga tgattgccag cgccatcaac gcgtatttac gccacggtga
     2101 gcaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgccg cctgcgactg
     2161 agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga tcacggtcac
     2221 gccgcgaatc accacatcat caaaatcaac agtgatattg ccgtctttct ctttgcacaa
     2281 cagtttcagc agattaacga ggttagtacc gtaaagctgt gaggattgcg tcggcagacg
     2341 gcccggaaga tcggtataac caatcacttt gacaccattt tccgtactga agatttcacc
     2401 cggcacggtg tattcacagt tgccaccgtt ttgggctgcc aggtcgacaa tcacactgcc
     2461 cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt tgcctggaat
     2521 aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga gttccatttc
     2581 cgctttgatg aacgcgtccg acatcacttt ggcatagcca tcgccgctgc ccgcttcctc
     2641 tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt tcacttccgg
     2701 gcgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa tggcggccag
     2761 acctgcaaca ccggcaccaa tcaccatcac ttttgccggt ggcactttcc cggccgcagt
     2821 gatttgcccg gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg cgcgataacc
     2881 ggcgatattc gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga tacgcggcac
     2941 agagtccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta attccggatt
     3001 ctgcgcaggc cagataaaac tcaccagcgt tgtcccggga ttcagtaacg caatttcatc
     3061 atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat tcccttctac
     3121 aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt gacccgcgcc
     3181 gctctctacc gcgacggtaa aacccagttt cagcagctgt tccactgttt ttggcgttgc
     3241 tgcaacacgg gtttcattgg ttaaccgttc tcttggtatg ccaattcgca tgatattccc
     3301 ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag ccgctcatgt
     3361 acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata acgtactgaa
     3421 aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg caaaatatta
     3481 acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga ctgataattg
     3541 tcttcgacgg tcgggtaaaa cgagacatcg ccccggcacg aatcactact taacattaaa
     3601 ttaacttata caattcagtt gcttcagtag taatgatgct gatacggctg ttttttaagc
     3661 atagacggtc atttgagcag gattaaaatt ggtttaagga atgtgatatg aaaaatgacg
     3721 cagacagtta caccgtttaa atgcaataat cagccacgtt tctcgttaat aacaatacca
     3781 gtacctggtt tgcgcaaggc gaaggattat ttttatgaag cttaagaaca ccctcctggc
     3841 gtcggcactg ctttctgcta cggcattctc cgttaacgca gcaacagaac tgacaccgga
     3901 gcaagcggca gcggttaaac cttttgaccg tgtagtggtt accggtcgtt ttaatgctat
     3961 tggcgaagcg gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg cctcttttta
     4021 tgttgtcgac acttctgatt ttggtaacag tggtaactgg cgtgtggtcg ctgacctcta
     4081 taaagccgat gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg gtgttgtcga
     4141 actgccgaaa gatcaggctg ttctgattga accgtttgac acggtcaccg tccagggctt
     4201 ctatcgtagc cagccagaag tcaatgatgc catcaccaaa gcggcaaaag cgaaaggtgc
     4261 ctactctttc tacatcgttc gtcaaatcga tgccaaccag ggcggcaacc agcgtattac
     4321 tgcattcatc tataaaaaag atgctaagaa acgtatcgtc cagagcccgg atgtgatccc
     4381 ggcagattcc gaagcaggac gtgcagctct ggctgccggt ggcgaagccg cgaagaaagt
     4441 tgagatcccg ggtgttgcga ctaccgcatc accaagttct gaagtcggtc gcttctttga
     4501 aacccagtca tcaaaaggcg ggcgttacac cgtcacgctc ccggatggca ctaaagtcga
     4561 agaactgaac aaagcgacag cagcgatgat ggtcccgttc gacagcatca aattctctgg
     4621 caactacggc aacatgaccg aagtctccta tcaggttgcg aaacgtgccg cgaagaaagg
     4681 tgctaagtat taccacatca cccgccagtg gcagaaacgt ggtaataacc tgaccgtcag
     4741 cgcagatctg tataaataac tattcagtta ctcctataag gcggcttgat gccgcctttc
     4801 gcatttttag tcacaaattt ccccgcgacc attgcatgca gacacatctc ttcgtaaaat
     4861 cccgcgcttc agtggcaaac tccattttta tgcgttttgc cctaatattt attcttctta
     4921 tcacgtttta atcactggat atcgatggaa aagaaactgg gactgagcgc actcaccgcg
     4981 ctggtattaa gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa tatggcggca
     5041 gttgccagcc cggcagcact gctcatcggc tggggtatta ctggcgctgg cattttattg
     5101 ctggcctttg ccatgctgat cctcacgcgc attcgtcccg aacttgatgg cggtatcttt
     5161 acctatgccc gcgaagggtt tggcgagcta atcggctttt gttccgcatg gggatactgg
     5221 ctgtgcgcgg tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc gttaagcttt
     5281 tttacggaca cgccggaatt gcgcctgttt ggcgatggta atacctggca atcgatagtc
     5341 ggtgcatcgg ctttattgtg gattgttcac tttttgatcc tgcgcggggt gcaaaccgct
     5401 gccagcatta acctggtggc gacactggca aaattgttgc cgctgggtct gtttgttgtg
     5461 ctggcgatga tgatgttcaa actggatacc ttcaagctcg acttcaccgg acttgcactt
     5521 ggcgtatctg tttgggaaca ggtgaaaaac accatgctga tcaccctgtg ggttttcatt
     5581 ggtgtggaag gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga tgttggcaaa
     5641 gcgacactgc tggcggttct ctccgctctg ggcgtttact tgctggtaac gctgctttcg
     5701 ctgggtgtgg tggcacgtcc tgaactggct gaaattcgta acccgtcaat ggccggtctg
     5761 atggtggaaa tgatgggacc atggggcgaa atcatcatcg ctgccggttt gatcgtttcc
     5821 gtttgcggcg cgtacttaag ctggaccatc atggcggcag aagttccgtt cctggctgcc
     5881 actcataaag cattcccacg catttttgcg cgccagaacg cacaagctgc gccatccgcc
     5941 tcactgtggt tgaccaacat ctgcgtgcaa atttgtctgg tactcatctg gctgaccggt
     6001 tccgattaca acacgttgct gaccatcgcc tcagaaatga ttctggtgcc ctatttcctg
     6061 gtcggcgcgt tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt aggtgtcggt
     6121 gcctgcattt atggcttatg gttattgtac gcctctggtc caatgcactt gctgttatcc
     6181 gttgttctgt atgcaccggg gctgctggtt ttcctgtatg cgcgcaaaac gcatacacat
     6241 gataatgtac tgaaccgtca ggaaatggtg ctgattggca tgctgttaat tgcctcagtt
     6301 ccggcgacct ggatgctggt gggataacgt tcatcccatc gttggatagc aaaggagata
     6361 acgatggata aaacccagcc cttgccaata ttaattactg gcggaggtcg tcgcatcggc
     6421 ctcgccctcg catggcattt cattaatcaa aagcaaccgg tgattgtcag ctatcggaca
     6481 cactatccag ccattgatgg actgattaat gcaggtgcgc agtgtattca ggctgatttt
     6541 tcgaccaacg acggtgtgat ggcgtttgcc gatgaagtac taaaaaccac ccatggtctg
     6601 cgtgctattt tgcataacgc cagtgcgtgg attgcggaaa aaccgggtgc gccactggcc
     6661 gacgtactgg cttgcatgat gcagatccac gttaataccc catacctgct caaccatgcg
     6721 ctggaaagat tactgcgtgg gcacggacac gccgccagcg atatcattca ctttaccgat
     6781 tatgtggtgg agcgcggtag cgacaaacat gttgcgtatg ctgcaagcaa agcggcactg
     6841 gataatatga cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt gaattctatt
     6901 gcgccatcgc tgatcctgtt taatgaacat gatgatgccg aatatcgaca acaggcgctg
     6961 aataaatcac tgatgaaaac cgtgcctggc gagaaagaag tgatcgacct ggtcgattac
     7021 ttacttacca gttgctttgt caccggacgc agtttcccac ttgatggcgg tcgtcatctg
     7081 cgttaatgca gctttatcca gcatataatc aacacccatg cgcttatccc ccaacacgca
     7141 acagaaccaa caaaagcggc gggcaagcgc atcattccgg tgaaatacca cagcgacacc
     7201 aggtaaacaa aatagggaat aatcgaccac atactaaaaa tgatggttgc gcgtaaggct
     7261 tcaatgccgc gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt cgggaaaagt
     7321 ggaatcagcc cggcgatata ataatttttc gtttttgcta aaacaccaat caacagcact
     7381 accagcgcac cgagcgcggc tttgattacc agccccatct ttttacctta acacttccat
     7441 aacaagtcat cagtagaata cctgatgaaa acttgtttag aaacgattga tagtaagtaa
     7501 aaacagcgcg gtgtattgtg acgtttttat atctaccgtg aatgttatga acactatcgt
     7561 atttgtggaa gatgatgcgg aagtcggttc actgattgcc gcgtacctgg caaaacatga
     7621 tatgcaggtt accgtagaac cgcgcggcga ccaggccgaa gaaaccattt tgcgagaaaa
     7681 tccggatttg gtgttactcg acatcatgct accaggcaag gacggcatga ccatttgtcg
     7741 tgatttacgc gcaaagtggt ctggaccgat tgttcttcta acctctctcg atagcgatat
     7801 gaaccacatc ctggcactgg aaatgggtgc ctgcgactat attctcaaaa cgacgccccc
     7861 tgctgttttg ctagcgcgtt tacgtttgca tttgcgtcag aatgagcaag ccacactgac
     7921 caaaggtctt caggaaacgt ctctgactcc ctacaaagcc ctgcatttcg gcacgttaac
     7981 tatcgacccc atcaaccgcg tagtcacctt ggctaacact gaaatctcgc tctcgacagc
     8041 tgatttcgaa ttattgtggg aactagctac gcatgccggg caaatcatgg accgcgatgc
     8101 attgctgaaa aatttacgcg gcgtcagtta tgacggactg gatcgtagcg tggacgtggc
     8161 tatttcgcgg ttaagaaaaa aactgctcga taacgccgca gaaccttatc gcattaaaac
     8221 tgtgcgtaac aaaggctatc tttttgcgcc tcatgcatgg gatagtgaca ataaacaatc
     8281 agatgactaa tatttgttca tctgaaaaag agaacaatta acgaattcaa ttaataagag
     8341 cttattcaaa ttccaaaccc cctacatttg atatgaatat gagaaaaaat ttctctataa
     8401 tcgcctcgtc cctgcgtctt atggcgcaga ttttttacaa caaactcttt ccggcatttc
     8461 aggtgctgaa tgaggcaaga ggcttaaaaa acggttacaa ctttcacata tattgtgctt
     8521 tttttgatct gctcatgggg gattttcaaa acggatcaaa agagcgaatc cctttccaaa
     8581 agtgttttta tcgcgatttt cctgggggct atcattggta tctccctgca tttcattcct
     8641 gagacaagca gtgaaaccgt tatcgaatgg tataatattg ctggtaacgg ttatgttaat
     8701 ttattaaaac ttgttgccgt tccactcata ttcatttcga ttctttcagc gattaataag
     8761 ctcgaaaata gcgctggtat tggcaagatg tctttaacta ttgttgcctg tatgttgtgt
     8821 ttagtcatga ttgcaggttt agttggtctg gctattgcca gtgcaattgg ccttgatgcc
     8881 agcgcctttg gtcatatgca atcctcttta accagcgatg acgtctcgaa aacagctgcg
     8941 gtatctttac cgcgactcat tacgtcattg atcccaacca acctcttcct tgatctgacc
     9001 ggggcaagaa gtgtttcggt tattggcatc gtcatcttta caattcttgc tggtattgcg
     9061 ctgttaaaag taaagaaaga tgcaccagca gagggcgaaa aattgagcgc ggggatcaac
     9121 gcgattcaaa tatgggtaat gaaaatggtc cgtattgtca ttgcgctgac gccttatggg
     9181 gtaatggcac taatggcgaa agtcttctca acgtatcagc tttcacagtt taccaatttg
     9241 ctcgggttca tcgtcgcctg ttatctggcc gtttttgtga tgtttattgt tcactcgggc
     9301 attttgctgt taataggtaa aaaccccgtt aactattaca agaccgtctg gcaagtactt
     9361 acctttgcgt ttgtttctcg cagtagtgcg gcttcaattc ctctggctat ttctgcattg
     9421 gaaaaattgg gggttccaaa tacgattgct aatattgcag cgtcttttgg ttccagcatg
     9481 ggacaaaatg gctgcgctgg gatttatcct gccgtgatgg tggcgatgat tgctccaaca
     9541 atgggtatcg atcccttctc attgcaattc ctcgcaactc ttttacctgc catcgcgctt
     9601 ggctcaatag gggtagcagg cgtaggtggt ggtgggacat tcgcggcgct gattgtcctt
     9661 tcaacactta atttccctgt cgcattagta gttattttta ttgccataga gccgatcgtt
     9721 gatatggcaa gaacagcgct taatgtgaat ggttcaatga tgtccggtgt aatatcagcc
     9781 cgtcttctta aggata
//
LOCUS       DADLSB010000141         9734 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.141, whole genome shotgun sequence.
ACCESSION   DADLSB010000141 DADLSB010000000
VERSION     DADLSB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.141"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JN331_003247"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JN331_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY7261315.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JN331_003248"
     CDS             1828..3147
                     /locus_tag="JN331_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY7261316.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3524..4669
                     /locus_tag="JN331_003249"
     CDS             3524..4669
                     /locus_tag="JN331_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7261317.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFYSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDCDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKGSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="JN331_003250"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="JN331_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5873
                     /gene="ddpX"
                     /locus_tag="JN331_003251"
     CDS             5469..5873
                     /gene="ddpX"
                     /locus_tag="JN331_003251"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HAY7261318.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACVDNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRRPQQAQAMLWQACPDPQYVVDVTVGSNHSRG
                     TALDLTLRDDVEVVNDAKRGNVRRTPTKTNAI"
     gene            5818..6714
                     /locus_tag="JN331_003252"
     CDS             5818..6714
                     /locus_tag="JN331_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY7261319.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6711..7697
                     /locus_tag="JN331_003253"
     CDS             6711..7697
                     /locus_tag="JN331_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7261320.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7690..8616
                     /locus_tag="JN331_003254"
     CDS             7690..8616
                     /locus_tag="JN331_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7261321.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKSTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTASAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHECEVRQSLAIREDGRELRCWRAL"
     gene            complement(8672..9103)
                     /gene="osmC"
                     /locus_tag="JN331_003255"
     CDS             complement(8672..9103)
                     /gene="osmC"
                     /locus_tag="JN331_003255"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY7261322.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            9449..9664
                     /gene="bdm"
                     /locus_tag="JN331_003256"
     CDS             9449..9664
                     /gene="bdm"
                     /locus_tag="JN331_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HAY7261323.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
BASE COUNT         2414 a         2364 c         2419 g         2537 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cttggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccacca
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctattc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggattgcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagg ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg ccgcccacaa caagcacagg
     5701 cgatgttgtg gcaagcctgc ccagacccgc aatatgttgt tgatgtgacg gtcggttcta
     5761 atcacagccg tggcacggcg ctcgacctga cgcttcgtga tgacgtggag gtggtgaatg
     5821 atgctaagcg aggaaacgtc cgccgtacgc ccacaaaaac aaacgcgatt taacggtgca
     5881 aaactggtat ggatgctgaa aggcagtccg ctcaccgtga ccggcgcagt catcattgta
     5941 ttaatgctac tgatgatgat tttttcaccg tggctggcga cgcatgatcc caacgccatt
     6001 gatttaaccg cccgcctttt gccgccttct gcggcgcact ggtttggcac cgatgaagtg
     6061 ggacgcgatc tgtttagccg cgtactggtc ggcagtcagc aatcaattct cgccggatta
     6121 gtggtggtcg ccattgcggg tatgattggt tcgctactcg gatgtctatc cggtgtgctt
     6181 ggcggacgcg cagacgccat catcatgcgc atcatggaca ttatgctgtc gattccttcg
     6241 ttggtactga caatggcact ggctgccgct ctcgggccga gtttatttaa cgccatgctg
     6301 gcgattgcta ttgtgcgaat tcccttttat gtgcgcctgg cgcgggggca agcattagtt
     6361 gtacgccagt atacctatgt tcaggcggcg aaaacctttg gcgcgtctcg ttggcatctg
     6421 atcaactggc atattttacg taactcccta ccgccgctga tcgtgcaggc atcgctggat
     6481 atcggtagcg cgattttaat ggccgccacg ttgggattta ttggcctggg tgctcaacaa
     6541 ccgagtgctg aatggggcgc gatggtagcg aatggtcgca actatgtact cgatcagtgg
     6601 tggtattgcg catttccggg ggcagcgatt ttgcttaccg ccgtcgggtt taatctcttt
     6661 ggtgatggta ttcgcgatct gcttgacccg aaagcaggag gaaagcagtc atgacccaac
     6721 ccgttctgga cattcaacag ctgcatttga gtttccccgg ttttaacggt gatgttcacg
     6781 cgctcaacaa tgtgtccttg cagattaacc gcggcgaaat tgtcggtctg gtgggagaat
     6841 ccggctcagg taaatcagtc accgcaatgc tgattatgcg tctgctaccg acgggcagtt
     6901 attgcgtaca tcgggggcag atttcactgc tgggagaaga tgttcttaat gctcgggaaa
     6961 aacagcttcg tcagtggcgc ggcgcacgag tggcgatgat ctttcaggaa ccgatgaccg
     7021 cccttaatcc gacacgtcga ataggtcttc agatgatgga cgtaatccgc catcatcaac
     7081 caataagtcg tcgggaagcc agagctaaag cgattgccct gctggaagag atgcaaatcc
     7141 cggatgccgt ggaagttatg tcgcgctatc cgtttgagct ttcaggtggt atgcgccagc
     7201 gggtaatgat tgcgctggca ttctcctgcg agccgcaatt gattattgcc gacgaaccga
     7261 cgacggcgct ggacgtcaca gtacagttgc aggtactgcg tttgcttaaa cataaagcac
     7321 gcgccagtgg aactgcggta ctgttcatca gccatgatat ggccgtggtg tcgcaactgt
     7381 gcgatagcgt ttacgtgatg tatgccggaa gcgtaattga aagcggcggg acggcagacg
     7441 ttatccatca tccccggcat ccgtatacca ttggtttgct gcaatgcgca ccagaacatg
     7501 gagtaccacg ccagccatta cccgccattc cagggacggt accaaacctc acccatttgc
     7561 ctgacggctg cgcttttcgc gatcgttgct atgcggcagg tgcacagtgt gaaaacgtcc
     7621 cggcgctgac agcgtgtggt gacaacaacc agcactgcgc ctgttggtat ccgcagcagg
     7681 aggtcataag tgtctgacac gttattaacg ttacgcgacg tccatatcaa ttttccggcc
     7741 cgtaaaaact ggcttggtaa atctacggaa catgttcatg ccattaatgg tattgattta
     7801 cagatccgcc gtggtgaaac cttagggatc gtcggcgagt caggctgcgg caaaagcacc
     7861 ctcgcacagc ttttaatggg catgctgcaa ccgagccacg ggcagtacat ccgttcaggc
     7921 tcacaacgca ttatgcagat ggtgtttcag gacccgctct cttcgcttaa tccgcgctta
     7981 ccggtgtggc gcatcatcac agaaccgctc tggatagcta agcgtagtag tgaacaacag
     8041 cggcgagcgt tggcagagga gttggctgtg caggtgggta ttcgtccgga gtatctcgac
     8101 cgcctgcctc atgcgttctc cggcgggcag cggcaacgca tcgccattgc cagagcactc
     8161 tcttcgcagc ctgacgtgat tgtgcttgat gagccaacct cggcgctgga tatctccgtg
     8221 caggcgcaga tcctcaattt actggtaacg ctacaggaaa atcacgggct gacttatgtg
     8281 ctgatttcac acaatgtctc ggtgatacgt catatgagcg atcgggtggc ggtgatgtat
     8341 ctcgggcaga ttgtagaact gggcgacgcg caacaggtgt tgactgcttc ggcacatcca
     8401 tacacccgat tattgctgga ttccctcccc gccattgata aaccgctgga ggaagaatgg
     8461 gcattacgta aaacggatct gccgggaaac cgcacgttgc cgcaaggctg ttttttctac
     8521 gaacgttgcc ctctggcaac ccacgaatgt gaagtccggc aatcattagc aataagggag
     8581 gacggacgtg agctccggtg ctggcgggcg ctgtagagat gctaacaacg catcaggcat
     8641 tcacgcgcct gacgcgtcat ccggcaatgc tttacgattt caactggtaa tccagcgtaa
     8701 tttccgcttt cagtacctga gagaccgggc atcctgcttt tgctttctgg atgatgccgt
     8761 caaaggtaga ggcatcaata cccggcaccg caacttcact cttcagtgcg attttcgtaa
     8821 tcgcaaaacc ggcatccact ttatccagcg acacatcggc ggtggtatca attgatgttg
     8881 gcgtgaatcc cgcttccccc agcattaatg aaagcgccat tgagaaacat gcggcatgcg
     8941 ctgcgccaat cagttcttca gggttggttc ctttttcgcc ttcaaaacgc gtgttaaatc
     9001 catacggctg ttggttcagc acgccgctct cggtggatac tgttcccttc ccgcgtttga
     9061 tatcgccttc ccagtgtgcc tgacctttct tatggattgt cattgttgct ctcctgtggg
     9121 cttaaaaagg aaattataga ctacgtgaga aacagcacga ggataagcag gatattccga
     9181 ataaaatacg gcagaaaata ttacttctgc acttaaatta aattcccaca atagataacc
     9241 gggaatatat atctcaagag atattgcatc aatatcaatc aactcaccac aataataata
     9301 cctatcaata tcatggggtt aaaatataaa aatcatcaac taggactaat ctcaacaacg
     9361 aagtggtaaa tatttgccat gctgaatgtg ctttataaat ctgcgatccg tagcagacac
     9421 cataaataca cagacacgga gaatcactat gtttacttat tatcaggcag aaaattcaac
     9481 agcagaaccc gctctggtta atgccattga gcaaggcctt cgggcagagc acggtgttgt
     9541 cactgaagat gacattctca tggagctgac caaatgggtg gaagcttctg ataacgacat
     9601 cctcagtgat atctaccagc aaacgatcaa ttatgtggtc agtggccagc accctacgct
     9661 ttaaggtgct atgcttgatc ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta
     9721 cggcgatgtt gtcc
//
LOCUS       DADLSB010000142         9593 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.142, whole genome shotgun sequence.
ACCESSION   DADLSB010000142 DADLSB010000000
VERSION     DADLSB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.142"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..928)
                     /locus_tag="JN331_003257"
     CDS             complement(<1..928)
                     /locus_tag="JN331_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HAY7261324.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDADIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQSEAGITRLLSSVEKSLDQAV"
     gene            complement(932..1495)
                     /locus_tag="JN331_003258"
     CDS             complement(932..1495)
                     /locus_tag="JN331_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261325.1"
                     /translation="MGEFRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETSWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGTAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     TVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(1470..1859)
                     /locus_tag="JN331_003259"
     CDS             complement(1470..1859)
                     /locus_tag="JN331_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HAY7261326.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELLPFDGGIPVEQ"
     gene            complement(1846..2400)
                     /locus_tag="JN331_003260"
     CDS             complement(1846..2400)
                     /locus_tag="JN331_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261327.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSERDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKAAAQAALDGNAAGAESA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRASGESPGTELA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(2397..2804)
                     /locus_tag="JN331_003261"
     CDS             complement(2397..2804)
                     /locus_tag="JN331_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HAY7261328.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLTE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            2803..3036
                     /locus_tag="JN331_003262"
     CDS             2803..3036
                     /locus_tag="JN331_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261329.1"
                     /translation="MFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPRTV
                     GKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(3033..3974)
                     /locus_tag="JN331_003263"
     CDS             complement(3033..3974)
                     /locus_tag="JN331_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY7261330.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DISIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNQLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYGDGI"
     gene            complement(3986..4135)
                     /locus_tag="JN331_003264"
     CDS             complement(3986..4135)
                     /locus_tag="JN331_003264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261331.1"
                     /translation="MSSSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADGNAE
                     ISWKI"
     gene            complement(4119..4493)
                     /locus_tag="JN331_003265"
     CDS             complement(4119..4493)
                     /locus_tag="JN331_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261332.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRSYVTVN
                     LGSDFDASTIKKGDPVYVVVIH"
     gene            complement(4497..5717)
                     /locus_tag="JN331_003266"
     CDS             complement(4497..5717)
                     /locus_tag="JN331_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY7261333.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(5721..6464)
                     /locus_tag="JN331_003267"
     CDS             complement(5721..6464)
                     /locus_tag="JN331_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY7261334.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQP
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(6355..7821)
                     /locus_tag="JN331_003268"
                     /pseudo
     CDS             complement(6355..7821)
                     /locus_tag="JN331_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            complement(7821..9224)
                     /locus_tag="JN331_003269"
     CDS             complement(7821..9224)
                     /locus_tag="JN331_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY7261335.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINHDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            complement(9175..>9593)
                     /locus_tag="JN331_003270"
     CDS             complement(9175..>9593)
                     /locus_tag="JN331_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261336.1"
                     /translation="RRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
BASE COUNT         2168 a         2713 c         2334 g         2378 t
ORIGIN      
        1 gaacggcctg atccagtgat ttttcaacac ttgataacag tcgggtaata ccggcttcac
       61 tctggggtac tttcgtggtg ctggtataaa gcaggttata gaggttggtc tgcacataat
      121 tctgtaacca gtccaggccg tggcgttcat caaagaaatc gccgttagcc atcactccct
      181 gctggaggat agccgtatca ttctggtagt acacgaatac attgcagttt tttgcatcaa
      241 gtgccgatgc ctggctgact gtcagtgttt catacccgac acccggctcc tgcttaaact
      301 tgagcgtaat cgcggtatta ctgccattga aattaaccgt gaatgcccgg ccaaatgcag
      361 ataacgcagc gtatttatta cccgatgaat actgaataaa actgcgtgaa tatccggcgg
      421 ttttcagttt tgatgccaaa tcatcgctgg atgcagtctg caggcatttc tcatcgcttg
      481 tcgtaatcgc cagaatacgg cttacagaag aggattcgat cgccgcagcc actttcagcc
      541 agtctgcatc cggaatatct gcatcgtctg caatccccag cccataccat gaagtataat
      601 caagcatggc attcacagcc tgctccagcg tctcaggcgt ggcctgttcg ctgtctccct
      661 tcgttttcac ccaacgacca acaaaaacct cctgaggttt cggtgattgt gagaaaaaca
      721 cctgcgcagc cttatattct ggtgattcca cgccaaaatc ttttccaata tcttccgcag
      781 cagaataacg gcgaatgcgc tcacttaccg gaatgattgt ggacgggccg agaatgagta
      841 atgcaccaaa atttcgccct gatgctgcac gcggcgacat gatcacatca acattaacaa
      901 cgtttgatac aggcaagccc tgtgccatag cttaatctcc gaaaaagatg actggtgctt
      961 ccaccagcga tttaataccg tactcgcgca caaccttccg gcgcagacgc accgtcatat
     1021 cgtagcggcg gacccattgc tgattaataa gttcagggaa gggagtcaga cctgtgtaat
     1081 cgccaagaga cagccccagc gcattcagtg ctgcattgtt ctgcggtaca gatataccgt
     1141 cacgaaaccg ggacgcatac accatccccg ccgtaccata aaacgaagcc atacactcaa
     1201 tcgtttcatg ccgccagagc tgagagccat catcggtctg tctggtgaat gccggactgt
     1261 catcacctga ccatccgata accccaaacg cacaccagct cgtttcaacc ggtagcagtg
     1321 gcggctgctc tttctgccag cgcggacgaa ccatcccggc agacagaccg gaaacgttac
     1381 gcatccactg gcttaacagc ctgtcgagcg cttcgtcata atccggatcg ccactggttg
     1441 gtatcagcca tccgcgctct gtgctggtgt tattgctcaa ccggaattcc cccatcaaac
     1501 ggcagcaact cacaatgcgc ctgaacgaat ccggcaccat acgctgtata cgggtcgacg
     1561 aaagtcacac gataatcacg gccctgatac gtcacgatat cggcatcacg gccagtctgt
     1621 ccctgcgtca gtcgctcagt cgtcacaatc agaattgcac cactgattac ctgcccggcc
     1681 tgcatacgac ggttttccag agagcgatca acagtaacga ctccggcaaa ctgcttttta
     1741 acttcgctgt cgctgccgat cccatcctca tccaccgttt gcacacggcg tgttacccac
     1801 aaattgaagt cgcaaaaatc ggggtcaaaa agcacatctg ttacatcaag agtcggcatc
     1861 tttatccctc acaacatggg taatcgctct gcgatattgc ccggtgtcaa ttaatggttt
     1921 cgccagttcg gttcccggag attcgccaga agcacgccgg gcaagttcca gtgttgcccc
     1981 cttgcgcccc cgacgagccc gggcttcaac agtactgtca gcaagcggcg taaagccggt
     2041 aatggtcatg taacgcctga cgccattagc ggccagtgtt ccggcacggt tgagtgcgct
     2101 ttctgctccc gcagcattac catcaagtgc agcctgcgcc gcggctttga gctgcggcac
     2161 cgtctgctct tctgccgatt taacgccggg gaccaagtga ggtcgtggcg ggatgttctg
     2221 ctctggtgag ccgtattcgt tgaggtaacc gatgcccgca ttaccaaacg gaacatcatc
     2281 ccgctcgctg tcttccgaag ggatgccgac cagcacatct tttttggtta acgacctgag
     2341 cgcatccaga atggccttag cgttatccac cctcgttgtt acaccgcttt tgaaactcat
     2401 agctggcgac cgcctgcacc gaacatcgtg atcaactgat aaaattcagc gccatatcgg
     2461 gtgttattcc agaaacctgc atcaggattc agcgtcgcgc tggtgtcata gctgacgctt
     2521 accttgtcaa cggactttga ggactgaaca ccattggttg aaccgcccgg accaccagcc
     2581 agcatcgctc tgctgtctgc cgcccagagc gtcatgtagt gcgcaacgaa caatccggca
     2641 aagtacggaa acaacttttt gccggtgacg ttttcggtca gcagttcatc ggccagattc
     2701 agacgaaact ggatttgcgc ttcgggatat ttggcagggt cagcaaactg cgggaagtcg
     2761 cggcgaaaat cacttaccgc tggcagactt tgattctttg gcatttttta cctcgttacg
     2821 cgcgtctgtg gctttgccaa cggatacttc cgcgtgcgca cgagtgaacc agtgcgtggc
     2881 aacgtcttcc tccacaacat gacggccttt aacaaactcg cgccgagaac cgtcgggaag
     2941 cgtgagcaca aacggggtat gtacgtgtat tactgcatca ttttttgcca tcgggtcatc
     3001 cttaatggcc ccgccagggg gccatgtggc tgttaaatgc catcaccgta cgaaatggtt
     3061 tctttgtaca ctggctcgac tgcacccagc ttgccgtagt aagtgacgat ctgatacaga
     3121 ccgcgatact gcaccggcac gctctgaagc ggaaccagcg ggtagcggac gtatttttta
     3181 tcgttggtgt acgcaaccat gcgatcctta ttccccacac cacggccttt cagccattta
     3241 accgcgcgga tattcagcgg aacaccgttc tggtgatagc tgatggtgtt ggtctgaagg
     3301 tacgtcaaca gggactggtt acctgcagat gaaacgatga tgctggacaa cagagcaaac
     3361 tgctcaggcg ggatcagcaa atcacgcgga accacagagt aaccagaagc ggcccacgca
     3421 tcagacagca cctggttaat gcttgcgcgg atttcgtccg gtgttgaggt tgcccacgtt
     3481 ttggcagcgt tgttgacagg cacgccgtcc agggtaacaa ggcctttcag gtttaatgcg
     3541 gaatcgccaa catatacctg ttcatcgtta tccatctgcc atttcagttg cataccgtca
     3601 tacttctgcg tatcaatcgg gcggccgacc tgctgagcag cctgcaattc tatgaccgtc
     3661 cagccaagtt ccatccccca caggttcagc tggttaccgg atttgccgat atccacgttc
     3721 acgccagcaa tagcggttga gtctttgcct acccagtttt ttccattcgg atttgcgcca
     3781 gtacccgcag cggcgaagct ggtattcgtc cagctggaaa tgtcatctgc gatggagatg
     3841 tcttcacgca gttgaatatc gcggctccag gtgtacccca ccagtggcag gttcagcgtc
     3901 tggtcgagtc gctccagctc cccgatgaga aaggcaccag agctgtcaac ggttgcctga
     3961 tcaaaagtaa tcattcgtct gttccttaaa tcttccagga aatttctgca ttgccgtcag
     4021 catcaccggc acctgtgaat tcagcgttgg tcagcaccac gtttttgcca ctgactgacg
     4081 tggacatgaa tccacccagc ggcactttga tggattcatc agtggatgac gacaacgtat
     4141 accggatcgc cttttttgat agtgctggca tcaaaatcag aaccgagatt aacggtcacg
     4201 tagctacgct tcatggcgtc gcccgggaag ttcttgcctg ttcccacctg gcgaaccatg
     4261 tccggctgcg acgtggtcgg ataagggcgc acgtagatcc ccttcacctt gtctgcggta
     4321 tcaccatctg ccagcggcac gaaaaaaccg tcatcatcgt atttaccagc caggccatag
     4381 gcagcgaagg cgttatcgga tttaaggacc accggttcga cggttaagtc ctgcgggcga
     4441 gagacagccc cggcaatgcc aacaggcatc cggtacagaa atacattatt cattttttac
     4501 cctttacggt ttgcccagaa ttcagcgttt tgtttgttca gggaagcgat actggtcatg
     4561 cccatgttta ggcgctgtgc atcgccggtg gtggcgcggg tgtttcgccc tttggcaacc
     4621 tcagacacgg cattaaacgc catgtcgacc gattgtttcg gtaatttgcg gatatccgca
     4681 tcaccgacta tctggcgaac cagcgttttg tcagcagaag ccagaacctc gcgtttgaac
     4741 gcggtcggtt tcatcttacg gctcagatcg atacccggaa cgataacttc ggcacgccag
     4801 gctgagtcac cagtaatcgt ggtttcctct tcatcgtcct cgccgtcacc ggtcggatta
     4861 tcgtcaggct tattatcgtt atcgcccgtg gcatttcttt ccagcttagc cagcagggct
     4921 ttcagtaatg ttttgaggtc atcatcactg tcgccggttg gatctccgcc catctctggt
     4981 gctttgtccg gtagtggttg ctgcggggac aggttgatgt tgagattaac gccctgcggc
     5041 aaatccccct catctcctgt aaccgatgcg ggagccgact ccaccagttc gttcatggtg
     5101 tcggcatctc ctgtcttgat ggccgcacgc atgcggttcc accagttttt cttttgattt
     5161 gccattgtgt ctctgtctcc aattgcacaa cgatttccgg ctctgccttt agggacaaga
     5221 gccacatggt ttccggtaat atcgacctgc tcggctttac ctggctcggt ctgctcgtac
     5281 tccgcgtcat agccgcacga cacttcgcgc aggccatctt cgataagctg aatggcgctt
     5341 tcgtctttga cgataaggtc agccagcatc aaatcagact gctcacccgt cccgcgccgg
     5401 acattctgga ggtgcccgac agcaagctct ttccagttct cgggatttac cagccgcaca
     5461 ttcccgtttt catcttcagg atgcaggatc gtgatgctca tcccttcgaa tgaggcaagc
     5521 gtagccggat ggaatacctg ctcaggagaa cgcgtgacga ctatttcacc gaacttatcg
     5581 ggtttcagtt ttggcaggtc atcagcacca tagagctgct tacctgttcg tcctatcggc
     5641 acgtctttgc acagcaacga gccgtcagcc aggcggtatc gggtttcccc gagcctggtc
     5701 tcaaaaaagt atttcatggg ttatgctgcc cttttcattg cgaatggaac gcgaacaaac
     5761 acgatctctt tatagcagcg gcagtttggc agctcgcccg cgtggccggt catgccgtcc
     5821 aaagttggag gtgaatccca gcggacgaat ttacctttca tgtgatcgtg ggaatctcgg
     5881 acatcgccat catcggctgt acgccaaata taaccatccg agccaatagc tatcgctcgg
     5941 gccatatcca gcgcgcccgt tgctcttccc agttccgttc gggcaatcag atcggcccta
     6001 gacttagcaa catcgccggt agatgcaatc tctttagcaa actgctccgc ccgaccaccg
     6061 gtgacaacag cctctatcgc tttgttctgg atgtcgtaca ccctgtctgc ggcctcgaga
     6121 ggcagagatt tgatgtactt gacctgttcg gcgatgatgg gttgcatcac ctggcccaca
     6181 ggagcgcttt ccacaagatt gcggagctcg cgactgatgt tcttgctgtg ttgccgccaa
     6241 actttctcgt tctgccgggt taggtccgca gtaaagtttt ccgcgacctt tgtcgcccag
     6301 ggggtgatga tttcactgta acgctccagc gccgcaataa tttccgtgat actgtcattt
     6361 gaaccatcgt agcgaccatt tacgatgtct ccgaccgccc tcgctatcct gcgtaggctg
     6421 gttcgatacc ggatttccgc ctgacggctc ctgcggttcg tcatcaggtt cgccgatgcc
     6481 gggcggcgct tcatcttcgg cattctcgat gtcctcgtcg gtaatggatg ccccgatgcc
     6541 ggttacgtca gaattttctc gcaaatcagt catagcggct ttcagtgtca tcagaccatc
     6601 acccagcgcc gtactgattg cgttggtggt gtttaacgcc accgttgagc gatcgacatc
     6661 agacatttgc cagagcgggt taaactcaaa cgtgaaatcg tccggcagcg gcttgccaag
     6721 ttccgaacga tgcatgatgt ccagtatccg ccgcaccgga agacgtaaac gcctctcctg
     6781 caacgaactg atgcgatcgt aatagttggc aaggtctgca tcaccggtag aaaatccttt
     6841 cggggactgt ccgaacaacc gcaccagtgg gataccaaca gcgccactaa tctgttctgc
     6901 aaactgcgaa aggatgtcat ccagaccact gaagctgtac tgatgggttt caaacttatc
     6961 ccgcgagtcc atgagcgtca tgccttcatt actctggaac tgtcgaatca ggtcgatatt
     7021 cttcagcaac gcttcatacg caggaccacc aagtgcgata agctcgcgta gcttctccac
     7081 gctgtaggta cgcaaatgcg ccttgtagac cagctgcgcc gcgccgacag tagcgctgtc
     7141 gaacgcggta agacgatccc agatacgctc tacaaccgac attccccatt cgttctcggt
     7201 catcttctgc tgaaatggca acgtgacgcc atcaaagcga atcaggcgac tgtgatgaat
     7261 gcgctaggca ggaattcccg ttgctgtggt caccacatcg taaaactcag gtttacccag
     7321 gtccggcccc atatctttaa tgcggcgggt cagtgccggg tcgaccatcc agcggtcgag
     7381 cgggagaatc cccttaaact tgcccttacc gatggtttcg ggtcgcagcg gggtcattgg
     7441 tgcctgcccc tcaatcatga tgaaacccac cgcgccgccg tagaggcgcg accatttcag
     7501 cacgtcattc agcgcatccc agatttgcaa ctcatccagt tgtgattcga gaatgccacg
     7561 atcttttgca tcaatttccg aagtgatgcg aatgcctttg cgggtcatat catccgggat
     7621 agcatcgact gcttcgccga tgatccagga tgaacgatag gaccattcca ccagcatgcg
     7681 gttacgacta gtgaaattag cccggtaggt ggatgctgag tgctggttag gtgtctgcat
     7741 ccctacgcgg gcaataaaat tctcataacc atcagctgtg gcctgcacag ttcgccgcag
     7801 ggcttgtttg tttcgtgcca tcaggcctgt ctccccagct gttcccagat atccagcgat
     7861 gtatcgattg gcgcgaaggc cataatgaat gcatcagcaa cgtttggtga cggtatctcg
     7921 cgttttgcga ggtctttttt actttcgacc atcacacgtc cgttacggtc gaaatcacga
     7981 tgaggtgttg tcagttccag tttcagcttt tcaagcaacg gacaacgaga atctatgctg
     8041 atcagctcat ccacaggata ctgttctccg ttgttaatgg cgttaaacgt atttctgaaa
     8101 cggtcagcca ccagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     8161 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     8221 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     8281 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     8341 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     8401 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     8461 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     8521 gcatccactg cggcttctat ccaagacagt ttgatgattg ccgcatcatc atcagactcc
     8581 ggaacgccct catacacatg tttaaaacca tccggatcac ggcgtcgcgc cgcgtcgata
     8641 accttaagca tggtgtcaga cagaaacgga ttttcgtcat ggttaatttt gcggatgaga
     8701 gtgccttcgg gcggatcaac aacgaagttg cgccagacga aatcagtaac aagtccgggg
     8761 ttgaatatga accagcattc cgaaccctct ttacggatcg ttggctccag aattttccac
     8821 tggtattccg tcagtgcgtg ggcttcttcc agccacaaca catcgatccc ctccagtgac
     8881 ttaatttctt cgatgttgcg ccataatcca taaaaaacaa attccgaacc agtaacccgg
     8941 ttaatgattt tgttgttcag aatacggaaa cggtgccgca gaccaaacct gtctatctga
     9001 attttgagca gggtatacac cgactcttcg attttattct ggatctgacg ggcacaacaa
     9061 aaacgcaggg tgtatttatt cgacagaaat atggcaatgc cagcggcatc ccatgatttt
     9121 gacgatgacc gaccaccata aagcactttg ttacgtgccc gcatagtcca gaaactacgt
     9181 aaagccggat tcagcgtcgg tttggatgtc agagtagaag tcattgaggt cacgctctcc
     9241 attgccatca tcaatacctg catcacggcg aagacgatca gcctccagag acaccttatc
     9301 agtggcggcc ttgcggtagg ctgtatcagc aaatattttt cctaccgtcg caagcgtgcc
     9361 aacgatggac tcaatacgaa ctgtattgcg catcattgct ttctcggcgg cgctgatatt
     9421 ttcaatcagc atcttccttt cctggtcccc attagcatct tccagcgaca ccaaccaccg
     9481 accaatattc tctgcagcga caaggttgtt agcacgaagg cgaaataatt cgtcctcgag
     9541 cgtcaacgct ttagcgtcct ctatcacctc atctttgagc agaaggcgac ggg
//
LOCUS       DADLSB010000143         9547 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.143, whole genome shotgun sequence.
ACCESSION   DADLSB010000143 DADLSB010000000
VERSION     DADLSB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.143"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..412)
                     /gene="mtlR"
                     /locus_tag="JN331_003271"
     CDS             complement(<1..412)
                     /gene="mtlR"
                     /locus_tag="JN331_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY7261337.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGP"
     gene            complement(412..1290)
                     /locus_tag="JN331_003272"
                     /pseudo
     CDS             complement(412..1290)
                     /locus_tag="JN331_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
     gene            complement(1292..1975)
                     /locus_tag="JN331_003273"
                     /pseudo
     CDS             complement(1292..1975)
                     /locus_tag="JN331_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            2512..2874
                     /locus_tag="JN331_003274"
     CDS             2512..2874
                     /locus_tag="JN331_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7261338.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2877..3962
                     /locus_tag="JN331_003275"
     CDS             2877..3962
                     /locus_tag="JN331_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7261339.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHLYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFILEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            4033..4641
                     /locus_tag="JN331_003276"
     CDS             4033..4641
                     /locus_tag="JN331_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY7261340.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVLVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFACTKPPKA"
     gene            4739..6130
                     /gene="selA"
                     /locus_tag="JN331_003277"
     CDS             4739..6130
                     /gene="selA"
                     /locus_tag="JN331_003277"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY7261341.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQCLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            6127..7971
                     /gene="selB"
                     /locus_tag="JN331_003278"
     CDS             6127..7971
                     /gene="selB"
                     /locus_tag="JN331_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY7261342.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLGIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            8161..9312
                     /gene="yiaY"
                     /locus_tag="JN331_003279"
     CDS             8161..9312
                     /gene="yiaY"
                     /locus_tag="JN331_003279"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY7261343.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLLHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         2249 a         2412 c         2575 g         2311 t
ORIGIN      
        1 agggtccaag gatttcgtcg tcggtaaagg cgtactcgtt gccgtcgtga tttagctctt
       61 cacgcaatgc catcagcagt tccgcatctt cgtattcctg gcggttaatg acgcccaacc
      121 cgtaaatgag ttttaaacgc acagaaagat cgcccagcgg accgtcgccg tcgagtaacg
      181 gttctacagc atacttcacc gcgtaatcgt ctttgcggaa tacctgaagc accagaagat
      241 ttaccgcctc ggtcaaaagc tcgacggcgg tgatcagaaa gcttcgcacg gttttgccag
      301 cattcagacg ctcaagcaca cggttttcaa aggcctgggt ttgttccatt gttgcctgca
      361 tatctgacaa tctgttattc gggcgcaggg tgtcctgcgc ctggtccacc attattgcat
      421 tgctttataa gcggttaccg cctcggatac aacctcgctg ttggcatcaa gaccggaaat
      481 ctgtgccagc gccgcctgcg gacctttgtc agcgatcagt gctgccagtt cctgagcctg
      541 cggatcatct tcactgcgga agtgcattgc accagcaatc ccctgaatca gattcttgtg
      601 cggaaggctg tattccagcg taccgagcag tggcttgatc agacggtcgc cagcactcag
      661 tttacgcagc ggctgacggc ctacgcgctc tacatcatct ttcagatacg ggttctcaaa
      721 acgaccgagg attttctgga tgtacgccgc atgtttgtct gcgtcaaagc cgtagcgctt
      781 gatcagtacc gcaccacttt cttccatcgc accttttacc accgcgcgga ttttctcgtc
      841 gagaatagcg tcacgaatgg tctgatgacc ggccagtttt ccgaggtacg cggttatagc
      901 atgacccgtg ttcagggtga agagtttacg ttcgacaaat gccatcaggt tgtcggttaa
      961 ctccatgcct gggatgttcg gcagtgcgcc tttgaactgc gttttatcga caatccattc
     1021 gctgaaagtt tctaccgtca cttccagcgg atcgttagtt gccgaagccg aaggcggtac
     1081 gatgcggtca acggcggaat cgacaaagcc aacgtgctct tctacccacg ctttggcgtc
     1141 ttccggcagg gcgttcatca catgaccttt cagctgcgtg gtgccgcgta ccatgttttc
     1201 acaggcgatg atgttcagcg gggattcatt accttgttct ttgcgtttca ccagcccttt
     1261 ggcgatagcc ggagcaatac gttccagcac aaagcacgcc catacctgga cctgggttag
     1321 cttcaatcag gaagaagatt gatttaccca gttcatggga ctgctgaata cccagcggcg
     1381 agaagatacc gtggttaatg gcgttgttga ggaacaggat tttcgccggt tcaacaaaga
     1441 tagacgccag cggcagcatg tcatggacaa ccatgaagtt aacgcccgca gccagcattt
     1501 tggacagggc ttcaacaatc gggccaatgc cgaggaatgc cagaatagcg aggatcatcc
     1561 cgatgatgcc tgcggagaag ttattcacca gcatctcaaa accggatttg atcttaccgt
     1621 ctacccagcg gtcgaagtgc ttaatgcacc agccgcccag cggacctgca atcatagaac
     1681 cgaggaacat cggcatgtct gcgccgacga taacgcccat ggtggtaatg gcaccgacta
     1741 cgccgccacg ttcgccgcct accagcttac caccggtata accgatcagc agcggtagga
     1801 gataagtgat catcggcccg accagcttcg ccagcgtctc gttcggtaac caccctgttg
     1861 gaataaataa cgcggtgatg ataccccacg cgataaacgc gccgatattt ggcatcacca
     1921 tgttgctgag gaaacgacca aagctttgca ctttgatctt aatatcggat gacataaaaa
     1981 caccccttct tatgtttgct gtcgcgcagg ctggaagccc gaggtttttt gttaatgtgg
     2041 cggcagaggt agccggacct tgtagatact gcgaaatctg gcactgaatc ggttaactgt
     2101 ccagtcgacg ggcttttgtg tgatatcagt cacataaata tagggggtag agaggtaatt
     2161 gacgtgacac tcatcacaaa acgcctgtgt aaaaaagaaa caaggcggtg attttcgcta
     2221 aaaatggcat ttaaatgtga gtaatgtcac atttttgttt tgatggtttg ttgaatcttt
     2281 gtgatctgaa tcacaagata ttttcttacg gcgttcacct gatgtgatct acagcatgtt
     2341 atgccttcgt catacaatcg ttatgtaact aagcaactca tcacctctaa aaattattct
     2401 ttggtgttat ggcgagaatc gcgaacccgg attattgtca aaatatcagc ctgactccct
     2461 tctggctgcc agccaaaaaa caacactgat aaacgtgaat ccgaggcaga tatgtttcta
     2521 aactatttcg cgttgggagt gctgatcttc gtctttctgg tgatttttta tggaatcatc
     2581 gcgatacatg acatccctta tctgattgcc aaaaagcgca atcaccccca tgccgacgct
     2641 attcatacgg cgggctgggt gagcctgttt actctgcatg ttatctggcc gtttctgtgg
     2701 atctgggcga cgctctatca accggagcgt ggctggggta tgcagtcaca tgttgcgtcg
     2761 caggagaaag cgactgaacc ggaaatcgcc gcactttctg accgaatttc ccggctggag
     2821 catcaactcg ccgccgagaa aaagactgac tattccacgt tcccggagat ctaattatgg
     2881 atctattgat tgttttaact tacgtggcgc tggcgacggc ggcgctggga ggcgtgtttc
     2941 tggtgagtgg tttgattttg ttgatgaact acaaccacct ttacactttt accgcgcaaa
     3001 aggcagtgat agcgatccct atcacgccac aggtgacggg aattgttact gaagtcactg
     3061 acaagaataa tcagcttatt caaaagggcg aggtgctttt taagctcgac ccggttcgtt
     3121 accaggcgcg agttgacaga cttcaggctg acctgatgac ggcgacgcat aatataaaga
     3181 cgctgcgtgc gcagctcact gaagcgcagg ccaacaccac ccaggtttca gcggagcgcg
     3241 accgtctgtt taaaaattat caacgttacc tgaaaggcag ccaggcggcg gtgaatccgt
     3301 tctcggaacg tgacatcgac gatgcgcggc aaaatttcct cgcgcaggat gcgttagtga
     3361 aaggctcggt ggcggagcag gcgcagatcc agagtcagct cgacagtatg gtcaacggcg
     3421 agcaatcgca gattgtgagc ttaagagcgc aacttactga agcaaaatat aatcttgagc
     3481 agactgtcat tcgcgcacca agcaatggct acgtcactca ggtactgatc cgcccaggta
     3541 catacgcagc tgccttgccg ctgcgtccgg tgatggtctt catcctcgag caaaaacggc
     3601 aaattgtcgc ccaatttcgg caaaactcgc tgttacgtct gaaacctggc gatgatgcgg
     3661 aagtggtgtt taacgcgcta cctgggcagg tgttccacgg caaattgact agtattttac
     3721 ctgtcgtgcc aggcggttct tatcaggcgc agggggtatt gcaatcatta acggtcgttc
     3781 ccggcacgga cggtgtgctg ggaaccattg aactggaccc taacgatgat atcgatgcct
     3841 tacccgacgg catctacgcc caggtggcgg tttactccga ccatttcagc catgtttcgg
     3901 tgatgcggaa agtgctgcta agaatgacca gttggatgca ttatctttat ttggatcatt
     3961 gagacttata tcacctgtgt tttgcgtgag cctgagccat actgatattt ttcctcgcac
     4021 aaaggactcg gcatgaaact cgtcggtagc tacaccagcc cgtttgtacg caaactttct
     4081 attctgttgt tagaaaaggg cataactttc gaatttatta atgaactgcc ctataacgcg
     4141 gataacggcg tggcgcaatt taacccgtta gggaaagtgc tggtgctggt gaccgaagag
     4201 ggcgaatgct ggtttgattc gccgatcatc gctgaatata ttgagttaat gaatgtcgct
     4261 ccggcgatgt tgccgcgcga tccgctggag tcgttgcggg tgcgcaaaat tgaggcgctg
     4321 gcggatggca ttatggatgc cgggctggta tcggtgcgtg aacaggcgcg tccggcggcg
     4381 cagcagtctg aagatgaatt gttacgccag cgggagaaaa tcaaccgcag tctggatgtg
     4441 ctggaaggat atctggtcga tggcacactc aaaaccgata cggtcaatct ggcgactatc
     4501 gccattgcct gtgctgtcgg atatctcaat ttccgccgcg ttgcgccagg ttggtgtgtc
     4561 gaccgcccac acttagtcaa actggtcgaa aacctgttta gccgcgaaag ttttgcttgc
     4621 accaaaccgc caaaggcttg atgcgcgata tgtcctcctg acccatctca cgttacaatc
     4681 cgtggttatg ttaaacgccc ttctccgtgt gagagggcct tgatcagcca ggtttcctat
     4741 gacaaccgaa acgcgttccc tctatagtca acttccggct attgatcgct tattgcgcga
     4801 tagctccttc ctttctttgc gtgatactta tggtcacacc cgcgtggtgg aattgttgcg
     4861 tcagatgctc gacgaagcgc gagaagtgat tcgtggcagc cagacgctgc ctgcgtggtg
     4921 tgaaaactgg gcgcaagaag tcgatgcccg gttgacgaaa gaggcgcaga gcgcgctgcg
     4981 tccggtgatc aacctgacgg gaaccgtgct gcataccaac cttgggcgag ctttacaggc
     5041 ggaagcagcg gtggaagccg ttgcgcaggc tatgcgttcg ccagtgaccc tcgagtacga
     5101 tctggacgac gccggacgcg gacatcgcga tcgggcgctg gcgcagctgc tgtgccgtat
     5161 tacgggggcg gaagatgcct gtatcgtcaa taacaatgcg gcggcggtgt tattgatgtt
     5221 ggcggccact gccagcggga aagaggtggt ggtatctcgc ggcgaactgg tggagattgg
     5281 cggcgcgttt cgtattcccg atgttatgcg tcaggcaggc tgtaccctac acgaagtagg
     5341 gaccaccaac cgcacgcacg cgaatgatta tcgtcaggcg gtgaatgaaa ataccgcact
     5401 gttgatgaaa gtacatacca gtaactacag cattcagggg ttcaccaaag cgatagacga
     5461 agctgaactg gtggcgctcg gcaaagagct ggatgttcca gtggtgactg atttaggcag
     5521 tggctcgctg gtcgatctta gccagtacgg tttgccgaaa gagccaatgc cgcaggagtt
     5581 gattgcggcg ggcgtcagtc tggtgagctt ctccggcgac aagttgttag gcgggccgca
     5641 ggcagggatt atcgttggta aaaaagagat gatcgcccgc ctgcaaagcc acccgctgaa
     5701 gcgtgcatta cgcgcggata aaatgaccct cgcggcgctg gaagccacgt tgcgtcttta
     5761 tttacaccct gaagctctga gtgaaaaatt accgaccctg cgcctgctta cccgcagcgc
     5821 agaggtcatt caaatccagg cacaatgttt acaggctccc cttgccgcac attacggcgc
     5881 ggagtttgcg gtacaggtta tgccatgtct ttcgcagatt ggcagtggtt cgctgccggt
     5941 tgatcgcctg ccgagcgcgg cattaacgtt tacaccccat gatggacgcg gtagccacct
     6001 tgagtcatta gccgcccgct ggcgtgaatt gccagtgccg gtgattggtc gtatttatga
     6061 cggacgattg tggctggatt tacgctgcct tgaagatgag caacggtttt tggagatgtt
     6121 gttgaaatga ttattgcgac tgccggacac gttgaccacg gcaaaacaac cttattgcag
     6181 gcgattactg gcgtaaatgc tgaccgtctg ccggaagaaa aaaagcgcgg catgaccatc
     6241 gatctcggct atgcctactg gccgcagccg gatggccgcg tgcctggttt tatcgacgtt
     6301 cccggtcatg aaaagtttct ttccaacatg ctggcgggcg ttggtggtat cgatcacgcg
     6361 ctgttggtgg tggcgtgcga tgacggcgtg atggcacaga cccgtgagca tctggcgatt
     6421 ttgcagctga ccggtaaccc gatgctgaca gtggcgctga ccaaagccga tcgcgtggac
     6481 gaagcgcgtg ttgatgaggt tgaacgccag gtaaaggagg ttctgcggga atacggtttt
     6541 gctgaggcaa aactgtttat caccgcagca accgaaggtc ggggaattga tgccctgcgc
     6601 gagcatctgc ttcagttgcc ggaacgcgag cacgccagcc aacatagttt ccgcctcgcg
     6661 attgaccgcg catttaccgt aaaaggtgcc gggctggtcg tcaccggtac ggcgttaagc
     6721 ggggaagtga aggtaggcga ttcactctgg ctgactggtg taaataaacc gatgcgtgta
     6781 cgtgcgctgc atgcgcaaaa ccagccaaca gaaaccgcca atgccgggca gcgtatcgcg
     6841 cttgacatcg cgggtgatgc ggaaaaagag cagattaacc gtggcgactg gctgcttgcc
     6901 gatgtgccgc cagagccgtt cacacgggtg attgtcgagc ttcaaaccca tacaccgctg
     6961 acccagtggc agccgctgca tattcaccac gccgccagcc acgtcacggg acgcgtttca
     7021 ctgctggaag ataaccttgc tgaactggtc ttcgacaccc cgttatggct ggcagataac
     7081 gaccgcctgg tattgcgcga tatctctgcc cgcaacacgc tggccggagc gcgcgtcgtg
     7141 atgcttaacc cgccgcgtcg cggtaaacgt aaaccagagt atctgcaatg gctggcttcc
     7201 ctcgccaggg cacagaatga tagcgaagcg ttagccgttc acctgcaacg cggcgcggtt
     7261 aatcttcctg atttcgcctg ggcgcgccag ctcaacggcg aagggatgcg cgaactgctg
     7321 caacagcctg gctatattca ggctggttat agcttgttga atgcgccggt tgccgctcgc
     7381 tggcaacgga aaattctcga cacattagcg acttatcatg agcaacatcg cgatgaacct
     7441 gggcctgggc gcgaacgtct gcgacgtatg gcgttgccaa tggaagatga agcgctagta
     7501 ctgttgctga ttgaaaagat gcgcgaaagc ggcgacatcc tcagtcatca cggttggctg
     7561 catctgccgg atcacaaagc gggcttcagc gaagagcagc aggccatctg gcaaaaagca
     7621 gagccgctgt ttggtgacga gccgtggtgg gtgcgtgatc tggcaaaaga gacgggtacc
     7681 gacgagcagg caatgcgcct gacgctacgc caggcggcgc agcaaggaat aattaccgcg
     7741 atcgtcaaag atcgttatta ccgtaacgat cggattgtcg aatttgccaa tatgatccgc
     7801 gatctcgatc aggagtgtgg ttcaacctgc gcggcggatt tccgcgatcg cttaggcgta
     7861 ggccgaaagc tgggaattca gattctggaa tattttgacc gcattggctt tacgcgtcgt
     7921 cgtggaaatg atcatttatt gcgcgacgca ttattatttc cggaaaaata aggaaatgat
     7981 taattaattt ttaaaataaa tcaatacaaa tttcttatga ttttaaataa agcacattgt
     8041 ttaatgaata caatgtgctt tttattagat taattttgat aattaataat aatgatcatg
     8101 ttttcgaatg aaaatcctca gtaagctgcc cacccttttt tacactttca ggagtgtgtt
     8161 atggcatctt caactttctt tattccttca gtgaatgtca tcggcgctga ttcattgact
     8221 gatgcaatga atatgatggc agattatgga tttacccgta ccttaattgt cactgacaat
     8281 atgttaacga aattaggtat ggcgggtgat gtgcaaaaag cactggaaga acgcaatatt
     8341 tttagcgtta tttatgatgg cacccaacct aaccccacca cggaaaacgt cgccgcaggt
     8401 ttgaaattac ttaaagaaaa taattgcgat agcgtgatct ccttaggcgg tggttctcca
     8461 cacgactgcg caaaaggtat tgcgctggtg gcagccaatg gcggcgatat tcgcgattac
     8521 gaaggcgttg accgctctgc aaaaccgcag ctgccgatga tcgccatcaa taccacggcg
     8581 ggtacggcct ctgaaatgac ccgtttctgc atcatcactg acgaagcgcg tcataacaaa
     8641 atggcgattg ttgataagca cgtgactccg ctgctttctg tcaatgactc ctctctgatg
     8701 attggtatgc cgaagtcact gaccgccgca acgggtatgg atgccttaac gcacgctatc
     8761 gaagcgtatg tttctattgc cgccacgccg atcactgacg cttgtgcact gaaagccgtg
     8821 accatgattg ccgaaaacct gccgttagcc gttgaagatg gcagtaatgc gaaagcgcgt
     8881 gaagcaatgg cttatgccca gttccttgcc ggtatggcgt tcaataatgc ttctctgggt
     8941 tatgttcatg cgatggcgca ccagctgggc ggtttctaca acctgccaca cggtgtatgt
     9001 aacgccgttc tgctgctgca tgttcaggta ttcaacagca aagtcgccgc tgcacgtctg
     9061 cgtgactgtg ccgctgcaat gggcgtgaac gtgacaggta aaaacgacgc ggaaggtgct
     9121 gaagcctgca ttaacgccat ccgtgaactg gcgaagaaag tggatatccc ggcaggccta
     9181 cgcgacctga acgtgaaaga agaagatttc gcggttctgg cgactaatgc cctgaaagat
     9241 gcctgtggct ttactaaccc gatccaggca actcacgaag aaattgtggc gatttatcgc
     9301 gccgcaatgt aatcatcatt ccaacaacgg ctggcaaatt gtcagccgct ttttcaacta
     9361 tctctgtaat ccttgcccgt aaattcgtga tagctgtcgt aaagctgtta ccgactggcg
     9421 aatatttcgc cagtcacgtc tacccttgtt atacctcaca ccgcaaggag acgatcatga
     9481 ccaataatcc cccttcagca cagattaagc ccggcgagta tggtttcccc atcaagttaa
     9541 aagcccg
//
LOCUS       DADLSB010000144         9529 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.144, whole genome shotgun sequence.
ACCESSION   DADLSB010000144 DADLSB010000000
VERSION     DADLSB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.144"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            20..157
                     /gene="sra"
                     /locus_tag="JN331_003280"
     CDS             20..157
                     /gene="sra"
                     /locus_tag="JN331_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HAY7261344.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            308..2005
                     /locus_tag="JN331_003281"
     CDS             308..2005
                     /locus_tag="JN331_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY7261345.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHSPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAAKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2139..3149
                     /gene="adhP"
                     /locus_tag="JN331_003282"
     CDS             2139..3149
                     /gene="adhP"
                     /locus_tag="JN331_003282"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY7261346.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3295..3579
                     /locus_tag="JN331_003283"
     CDS             3295..3579
                     /locus_tag="JN331_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7261347.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTSEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3807..4460)
                     /gene="fdnI"
                     /locus_tag="JN331_003284"
     CDS             complement(3807..4460)
                     /gene="fdnI"
                     /locus_tag="JN331_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY7261348.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4453..5337)
                     /gene="fdxH"
                     /locus_tag="JN331_003285"
     CDS             complement(4453..5337)
                     /gene="fdxH"
                     /locus_tag="JN331_003285"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7261349.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5350..8397)
                     /gene="fdnG"
                     /locus_tag="JN331_003286"
     CDS             complement(5350..8397)
                     /gene="fdnG"
                     /locus_tag="JN331_003286"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7810..7812),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7261350.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8629..9510
                     /gene="yddG"
                     /locus_tag="JN331_003287"
     CDS             8629..9510
                     /gene="yddG"
                     /locus_tag="JN331_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY7261351.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGVHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSALLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2290 a         2514 c         2420 g         2305 t
ORIGIN      
        1 acaggattga ggagttaaaa tgaaatcgaa ccgtcaggca cgtcatattc ttggactgga
       61 ccataaaatt tctaaccagc gcaaaatagt taccgaaggc gacaaatcca gcgtggtaaa
      121 taacccaacc ggcagaaaac gccccgctga aaagtaattc ataaccatca gtcctcaatg
      181 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
      241 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      301 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      361 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      421 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      481 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      541 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      601 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      661 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      721 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      781 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      841 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      901 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      961 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
     1021 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
     1081 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
     1141 agtcggcaca ctgatcgcag ccagtcgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1201 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1261 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1321 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1381 gcgcgaaaac ctcagtgatt gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1441 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1501 agagatcatc cgtgagatgc ataaacactc tccgcgtccg atcgtgatgc cgctgtctaa
     1561 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1621 gctggtcgcc actggcagcc cgtttaatcc agtggtatgg aaagataaaa tctaccctat
     1681 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1741 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1801 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1861 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggccaa
     1921 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1981 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     2041 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     2101 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     2161 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2221 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2281 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2341 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2401 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2461 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2521 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2581 ttaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2641 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcaa
     2701 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atgggtgcag
     2761 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2821 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2881 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2941 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     3001 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     3061 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     3121 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3181 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3241 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3301 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3361 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3421 accggaaaag cagctttgac gtcagaaatg gcaattaaac tttccgtggt gatcggcagt
     3481 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3541 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3601 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3661 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3721 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3781 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3841 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3901 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3961 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     4021 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     4081 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     4141 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4201 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4261 gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg
     4321 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4381 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4441 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4501 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4561 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4621 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacat gcgtaccacc
     4681 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4741 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4801 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4861 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4921 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4981 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     5041 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     5101 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     5161 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5221 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5281 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5341 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5401 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5461 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5521 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5581 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5641 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5701 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5761 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5821 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5881 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5941 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     6001 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     6061 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     6121 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6181 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6241 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6301 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6361 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6421 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6481 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6541 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6601 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6661 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6721 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     6781 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6841 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6901 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6961 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     7021 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     7081 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     7141 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7201 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7261 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7321 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7381 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7441 ttcgaaagca aaatcatcac gcaccagcag gctggcgttg gtgtaatgtt taacgtattc
     7501 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     7561 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7621 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7681 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7741 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7801 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7861 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7921 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7981 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     8041 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt tgacgtaatc
     8101 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     8161 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8221 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8281 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8341 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8401 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8461 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8521 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8581 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8641 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8701 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8761 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8821 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8881 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8941 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     9001 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tggggtacat
     9061 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     9121 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9181 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9241 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9301 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9361 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9421 agcgccctgc tatcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
     9481 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacg
//
LOCUS       DADLSB010000145         9519 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.145, whole genome shotgun sequence.
ACCESSION   DADLSB010000145 DADLSB010000000
VERSION     DADLSB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.145"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            58..1341
                     /locus_tag="JN331_003288"
     CDS             58..1341
                     /locus_tag="JN331_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7261352.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAWIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1593..2510
                     /gene="oxyR"
                     /locus_tag="JN331_003289"
     CDS             1593..2510
                     /gene="oxyR"
                     /locus_tag="JN331_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY7261353.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2493..3893)
                     /gene="sthA"
                     /locus_tag="JN331_003290"
     CDS             complement(2493..3893)
                     /gene="sthA"
                     /locus_tag="JN331_003290"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY7261354.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            4214..4864
                     /gene="fabR"
                     /locus_tag="JN331_003291"
     CDS             4214..4864
                     /gene="fabR"
                     /locus_tag="JN331_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY7261355.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4864..5223
                     /locus_tag="JN331_003292"
     CDS             4864..5223
                     /locus_tag="JN331_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY7261356.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(5263..6363)
                     /gene="trmA"
                     /locus_tag="JN331_003293"
     CDS             complement(5263..6363)
                     /gene="trmA"
                     /locus_tag="JN331_003293"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY7261357.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6477..6667
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6732..8576
                     /gene="btuB"
                     /locus_tag="JN331_003294"
     CDS             6732..8576
                     /gene="btuB"
                     /locus_tag="JN331_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY7261358.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8521..9378
                     /gene="murI"
                     /locus_tag="JN331_003295"
     CDS             8521..9378
                     /gene="murI"
                     /locus_tag="JN331_003295"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7261359.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLLA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2168 a         2291 c         2554 g         2506 t
ORIGIN      
        1 gcttaattat tttctgaaca gtcatcaaat cgattaattc cgctcaagga gctaaaaatg
       61 gacgttgatg tgtcaaccag tgttgcgggt aataaaccgc aacggattcg tcgtattcag
      121 acagtgaccc tggttttatt atttatggcg tggatcgtta attttctcga ccgttcgtca
      181 ttgagcgtgg caggcgaagc gattcgtggc gagttaggat tatcggcgac agagtttggt
      241 gttttgcttt ctgcattttc tctgtcttat ggtttttcac aactaccttc cggtattttg
      301 ctagaccgct ttggcccacg aatagtttta ggcgcaggct taatattctg gtcattaatg
      361 caggcattaa caggaatggt taattctttt agccacttta ttttaatgcg tatcggtctg
      421 ggtattggcg aagcgccatt tatgcctgcg ggagtaaagt cgatcaccga ctggtatgca
      481 caaaaagaac gcggcacggc gctggggatt tttaactcat ctaccgttat cggtcaggct
      541 atcgcgcctc ctgctcttgt attgatgcag ctggcatggg gctggcggac gatgttcgtt
      601 atcatcggcg tggcagggat tctggttggg atctgttggt acgcgtggta tcgcaaccgg
      661 gcgcagtttg tcctgactga cgaagaacgg acgtatctct ccgcgccggt taaaccgcgt
      721 ccacagctgc aatttagcga gtggctggcg ctgtttaagc atcggacaac ctgggggatg
      781 attttaggtt tctctggcgt caattatacc ggttggctct acattgcgtg gttacccggt
      841 tatttgcaag ccgaacaagg tttcagtctg gcgaaaaccg gttgggtagc ggcgattcct
      901 ttcctcgcgg cggcggtcgg gatgtgggtt aacggtattg tggtcgatcg gctggcgaaa
      961 aaaggctacg acctggcgaa gacgcgtaaa acggctattg tctgcggttt gatgatgtcg
     1021 gcattaggca cgttgctggt cgtgcaatct tcctcgccag ctcaggcggt agcgtttatc
     1081 tcaatggcgc tgttctgcgt gcatttcgcg ggaacgtctg catggggact ggtgcaggtg
     1141 atggtgtcag aaacaaaagt ggcttccatc gccgggattc aaaactttgg cagttttgtt
     1201 tttgcttcct ttgctccgat cgtaaccggt tgggtagtgg ataccacaca ctcgtttaat
     1261 ctggcgctgg ttatcgcggc ctgtgtgacg ttcaccgggg cgctgtgtta cttctttatt
     1321 gtcaaagatc gcattgagta atcgttacta tcaggctctc ttgctgtggg cctgtagaat
     1381 aaaaaaaagc ggatcctgga gatccgcaaa agttcacgtt ggctttagtt attcgagttg
     1441 agaaactctc gaaacgggca gtgacttcaa gggttaaaag aggtgccgct ccgtttctgt
     1501 gagcaattat cagtcagaat gcttgatagg gataatcgtt cattgctatt ctacctatcg
     1561 ccatgaacta tcgtggcgat ggaggatgga taatgaatat tcgtgatctt gagtacctgg
     1621 tggcattggc tgaacaccgc cattttcggc gtgcggcaga ttcctgccac gttagccagc
     1681 cgacgcttag cggccaaatt cgtaagctgg aagatgagct gggcgtgatg ttgctggagc
     1741 ggaccagccg taaagtgttg ttcacccagg cgggaatgct gctggtggat caggcgcgta
     1801 ccgtgctgcg tgaggtgaaa gtccttaaag agatggcaag ccagcagggc gagacgatgt
     1861 ccggaccgct gcatattggt ttgattccta cggttggacc atacctgcta ccgcatatta
     1921 tcccgatgct gcaccagaca tttccaaagc tggaaatgta tctgcatgaa gcacagaccc
     1981 accagttact ggcgcaactg gacagcggca aactcgattg cgtgatcctc gcgctggtga
     2041 aagagagcga agcattcatt gaagtgccgt tgtttgatga gccaatgttg ctggctatct
     2101 atgaagatca cccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg gcaggggaaa
     2161 aactgctgat gctggaagat ggtcactgtt tgcgcgatca ggcaatgggt ttctgctttg
     2221 aagccggggc ggatgaagat acacacttcc gcgcgaccag cctggaaact ctgcgcaaca
     2281 tggtggcggc aggtagcggg atcactttat tgccagcgct ggctgtgccg ccggagcgca
     2341 aacgcgatgg ggttgtttat ctgccatgca ttaagccgga accacgccgc actattggcc
     2401 tggtttatcg tcctggctca ccgctgcgca gccgctatga gcagctggca gaggccatcc
     2461 gcgcaagaat ggatggccat ttcgataaag tgttaaaaca ggcggtttaa accgtttaac
     2521 gcagctaccc gataggcttc cgccatcgtc gggtagttaa aggtggtgtt gacgaagtac
     2581 tcaatagtgt tgccgccacc tttctgttcc ataatcgcct gaccgatatg aataatttcg
     2641 gcagcgcgct cgccaaagca gtgaataccc agaatctctt ttgtttcccg atggaacaaa
     2701 attttcagcg tgcccacgtt catgccgacg atttgtgcac gtgccagatg tttgaactgg
     2761 gcgcggccca cttcatacgg cactttcatc gcggtcagct gctgttcggt tttgcccaca
     2821 gagctgattt ccgggatggt gtaaatgccg gtagggatat cttcaatcag atgtgcggtg
     2881 gcttcgcctt ttaccagcgc ctgcgcggca atgcgcccct ggtcataggc cgctgatgcc
     2941 aggctcggat aaccaatcac gtcgcccacc gcgtaaacgt gcggctgtgc ggtctgatac
     3001 atgctgttga ccttcagctg tccgcgactg tcagtttcca gcccaatgtt ctgtaacgcc
     3061 agcgaatcgg tattaccggt acgaccgttg gcatagagca ggcagtcagc tttcagtttt
     3121 ttacccgact tcagatgcat gatcacaccg tcgtcacagc tttcgatctt ctcgtactct
     3181 tcgttgtgac gaatcactac gccactgttc cagaagtgat aggagagaga atctgacatc
     3241 tcttgatcga gaaatgccag caggcgatcg cgggtgttga tcagatccac ttttacatcc
     3301 ataccgcgga agatcgacgc atattcacag ccgatcactc cagcaccata gataagtaca
     3361 tggcgcggtt cgtggtgcat gctaagaatt gagtcgctgt cgtaaatgcg tggatgggtg
     3421 aaatcaacat ctgttggatg atatggacga gagccgcagg caataacaaa tttttcagcg
     3481 gttagtgttt caacgctgcc gtccgggcaa tccagcgcca acgtatgctc gtcaacaaag
     3541 cgagcgttcc cctgcaatat ttcacagtga ttacgttcgt aaaatccctg acgcatgcgc
     3601 gtttgttgat taatcacgtt atcggcatgg ttaaggatat cggcaaaaga agagcggagc
     3661 agtcgggaat ggtcgctgta tagtgggttt tgattgaatt ctataatgcg gctgacggcg
     3721 tgacggagag ctttcgacgg gatggtgccc cagtgggtgc aaccgccgcc aacattttga
     3781 taacgctcga taactgcgac gcgcgcacct tgcttaacca ggcccattgc agcgccttcg
     3841 ccgccggggc cggaacctat tactatggca tcgtaatcgt aggaatgtgg catggtaggg
     3901 cttacctgtt cttatacata aaagcaacag aatggtaaca ttctatcgcg ggtaagccaa
     3961 ttgatccccg tcatttatct ggctatatcc tgagcggcct ttgctttgtc tgtttcttac
     4021 ttttgccctg acgttttatt ggatttttat cgacgatact ctccgtttaa gcggcaggtt
     4081 tccgctgtac gtaaaagaac cggccagtaa atatgtttta ttgcgttacc gttcattcac
     4141 aatactggag caatccagta tgttcattct ctggtatagt gccagcagta cttttggcaa
     4201 ggattcagac atcgtgatgg gcgtaagagc gcaacaaaaa gaaaaaaccc gccgttcgct
     4261 ggtggaagcc gcatttagcc aattaagtgc tgaacgcagc ttcgccagcc tgagtttgcg
     4321 tgaagtggcg cgtgaagcgg gcattgctcc cacctctttt tatcggcatt tccgcgacgt
     4381 agacgaactg ggtctgacca tggttgatga gagcggttta atgctacgcc aactcatgcg
     4441 ccaggcgcgt cagcgtatcg ccaaaggcgg gagtgtgatc cgtacctcgg tctccacatt
     4501 tatggagttc atcggtaata atcctaacgc cttccggtta ttattgcggg aacgctccgg
     4561 cacctccgct gcgtttcgtg ccgccgttgc gcgtgaaatt cagcacttca ttgcggaact
     4621 tgcggactat ctggaactcg aaaaccatat gccgcgtgcg tttactgaag cgcaagccga
     4681 agcaatggtg acaattgtct tcagtgcggg tgccgaggcg ttggacgtcg gcgtcgaaca
     4741 acgtcggcaa ttagaagagc gactggtact gcaactgcga atgatttcga aaggggctta
     4801 ttactggtat cgccgtgaac aagagaaaac cgcaattatt ccgggaaatg tgaaggacga
     4861 gtaatgaaac aagcaaatca agatagaggt acgctgctgc tggcgttggt tgctggctta
     4921 tcgattaatg gtactttcgc agcgctgttt agctccattg tgccattttc tgtattcccg
     4981 attatttccc tggtgctgac ggtttactgc ctgcatcaac gttatcttaa tcgcaccatg
     5041 tcggtaggct tgccgggtct ggcagctgcc tgttttattc tcggcgtact gctgtacagc
     5101 acggtagttc gtgcggaata tccggatatc ggctctaact tcttcccggc agtactctcc
     5161 gtcattatgg tgttctggat tggcgcgaag atgcgtaacc gtaagcagga agttgctgag
     5221 taatcggtat catgccggat gctggcgcat ccggcatggg ttttacttcg cggtcagtaa
     5281 tacgccgcac tccatatggt gcgtgtaggg gaactgatca aacagagcca gacgttcgac
     5341 cttgtgcgtc tggcttaatg tttccagatt tttgcataac gtttccggat tgcaggagat
     5401 atacagaata cgtggatacg cctgcaccat tttctcggtt tcactgtcca gaccgctgcg
     5461 cggtgggtcg acaaaaatgg tttcgaactg atagctcttt aagtcgatcc cttgcaggcg
     5521 gttaaactcg cgcacaccat tcatcgcctg agtaaattct tctgccgcca tacgaataat
     5581 ttgtacgtta tcaatatggt tagcggcaat gttgtattgc gcggcagcga ctgacggttt
     5641 ggcgatttcg gtggctaata cccgatcaaa attgcgcgcc agcgctaatg aaaagttacc
     5701 gttgccgcag tacagctcca gtaaatcgcc ttttgagcct ttggttacgt ccagcgccca
     5761 ttccagcatc tgaatattca tcgccgcgtt cggctgggta aagctgtttt ctacctgacg
     5821 atagatcatc tctttccctg cgaccggcag acgctcgtcg atgtaatctt gatccagcgc
     5881 gattttggtt ttcgttgccc gaccaatcag atgcacattc agattctgcg cgcgcagtgc
     5941 atcgcgcagt gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag
     6001 ggaaaccacc gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg
     6061 cagaacggga ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc
     6121 actggcggcg gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat
     6181 gtgatacagg tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact
     6241 gaccggcgag cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa
     6301 acgtaccact ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt
     6361 catgatgtat cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc
     6421 cagatcttga tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt
     6481 agcatccact tgccggtcct gtgagttaat agggaatcca gtgcgaatct ggagctgacg
     6541 cgcagcggta aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag
     6601 tcatcatctc ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg
     6661 catctggttc tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg
     6721 gatgctttac aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt
     6781 ccgcttgggc acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac
     6841 agccgcgcag cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct
     6901 ggcagtcgac ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa
     6961 acggcggttc aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc
     7021 tggtgttaat tgatggcgtc cgcctgaatc tagcgggggt gagtggttct gccgacctta
     7081 gccagttccc tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg
     7141 tttatggttc cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg
     7201 gaacggaaat ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc
     7261 agcaacaact gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg
     7321 gttatgatgt tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt
     7381 ttttaagtaa aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct
     7441 ttgttcgcgg ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt
     7501 caccgttgct cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata
     7561 acggcgaact gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact
     7621 acgatcccca ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca
     7681 ccgtccagtg ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact
     7741 ggcagaaaca gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta
     7801 ataccggcat ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag
     7861 cacgcagcga cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt
     7921 gggaattcat cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa
     7981 atctggggca actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac
     8041 agtgggaagg cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc
     8101 gtaacgatgt cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag
     8161 ggaaagcgcg gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacac
     8221 atactgtgag ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac
     8281 gccgtgctaa acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg
     8341 gtattactta tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc
     8401 aaaccgttaa aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct
     8461 ctcacctgac agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct
     8521 atggctacca aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca
     8581 cgtcccaccg tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc
     8641 cggcatctct taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat
     8701 ggcgaaaaaa gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa
     8761 gaacgttatc cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt
     8821 cttgcattac gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct
     8881 gctgcacgtc tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa
     8941 cgttcttata ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg
     9001 ggctcggcag agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg
     9061 gatgcactaa aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt
     9121 gtattgggtt gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag
     9181 ggaacccggc tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa
     9241 catgaagccc cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg
     9301 ccagaagctg aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa
     9361 ctggcagttt taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc
     9421 ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa
     9481 acacgccgac gggtcagcgg ggttctcctg agaattccg
//
LOCUS       DADLSB010000146         9430 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.146, whole genome shotgun sequence.
ACCESSION   DADLSB010000146 DADLSB010000000
VERSION     DADLSB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.146"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(233..991)
                     /locus_tag="JN331_003296"
     CDS             complement(233..991)
                     /locus_tag="JN331_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY7261360.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELDEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(999..2102)
                     /locus_tag="JN331_003297"
     CDS             complement(999..2102)
                     /locus_tag="JN331_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7261361.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2112..3293)
                     /locus_tag="JN331_003298"
     CDS             complement(2112..3293)
                     /locus_tag="JN331_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7261362.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3361..4386)
                     /locus_tag="JN331_003299"
     CDS             complement(3361..4386)
                     /locus_tag="JN331_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7261363.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4817..5038)
                     /locus_tag="JN331_003300"
     CDS             complement(4817..5038)
                     /locus_tag="JN331_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261364.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5291..6675)
                     /locus_tag="JN331_003301"
                     /pseudo
     CDS             complement(5291..6675)
                     /locus_tag="JN331_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018912.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6678..8300)
                     /locus_tag="JN331_003302"
     CDS             complement(6678..8300)
                     /locus_tag="JN331_003302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY7261365.1"
                     /translation="MPVALYPTIAPPAVSVSANYPGADAQTVQDTVTQVIEQNMNGID
                     NLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQQGISVEK
                     SSSSYLMVAGFVSDNPDTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQYAMRIWLD
                     ADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRLKNPEEFG
                     KVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDTAKA
                     IKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNMR
                     ATLIPTIAVPVVLLGTFAILATFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM
                     EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA
                     LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGST
                     GRYLLIYALIVAGMVVLFLRLPSYLKRIRVSF"
     gene            complement(8308..9375)
                     /gene="acrE"
                     /locus_tag="JN331_003303"
                     /pseudo
     CDS             complement(8308..9375)
                     /gene="acrE"
                     /locus_tag="JN331_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
BASE COUNT         2514 a         2450 c         2253 g         2213 t
ORIGIN      
        1 catcaaataa aacaaaaggc ccagtcttcc gactgagcct tttgttttat ctgttgtttg
       61 tcggtgaaca ctctcccgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
      121 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccagaca tcaaatcaag
      181 caaaaaggcc atccgaaagg atggcctttt tgcttttcga actaacattc aattaatgga
      241 ttacctgcga taaaaatgcc ctcgtacgct ctgatttagg atgcgcaaaa aattcatcag
      301 gtgtagcttg ttccactatc tccccacgat ccataaaaat tacccggtca gcgacggtac
      361 gtgcaaaccc catctcatgt gttacacaca acattgtcat acccgactgc gccagcccaa
      421 tcatcgtatc cagcacctct ttcaccatct ccggatcgag cgccgacgtt ggttcatcaa
      481 acaacataat tttcggcttc atacacagcg aacgcgcaat ggcaacgcgt tgctgctgac
      541 cacctgaaat ctgtccggga aacttatgcg catgttcggc aattctcacc cgctcaaggt
      601 aatgcatcgc cagagcttca gcctctttct taggcatctt gcgtacccaa atcggtgcca
      661 gggtacagtt ctgtaaaacg gtcagatgag ggaagagatt gaaatgctga aataccattc
      721 ccacttcctg cctgacgcgc tcaatattgc ggatatcttc atcaagttcg atgccatcta
      781 cgacaatccg tccctgttga tgttcttcca gatgattaat acaacgaatg gttgtcgatt
      841 taccggaacc agaagggcca cacagaacga tccgttctcc cggttgcacg gttaaattta
      901 tatttttcaa aacatggaat tgtccatacc atttatggac gttttccagc gtaatcatcg
      961 cgttagcagg ttgcagtaaa atttggctca tagtgtcctc aatgcggtgt acgcccggtg
     1021 ttaaaacgtt tttccagatg ttggctataa cgtgacatgc tgaaacaaaa gatccagtag
     1081 atcagcgcgg cgaaaacata cccttccgtc gacataccca accaggcggg atcaacggtt
     1141 gcctgctgaa cgctactgaa aagatcgaac aagccgatga tgatcaccag gctggtatct
     1201 ttgaagagtg cgatgatggt atttaccagc ccaggaatta ccagcttcaa cgcctgtggc
     1261 agaataacca gcccctgagt tttccagtaa cccaacgcca gcgactctgc cgcttcatat
     1321 tgccctttag gcagcgcctg taatccacct cgcacgactt ccgcaacata tgctgactga
     1381 aacaggatca cgccaaccag cgcgcggatc agtttgtcga tactggtgcc ttctgccata
     1441 aacaacggca gcatgaccga agacataaac aatacggtaa tcagcggtac gccgcgccag
     1501 aactcgataa aaatgaccga taagatacgc acaatcggca tatgggagcg gcgacctaat
     1561 gccagtaaga tcccccacgg tagcgccccc gcaatcccaa cggatgcaat aattaaagtt
     1621 agcgtcagcc cgccccattg ccgggtttca acccgctcaa gaccaaaaaa accgccatac
     1681 atcagccacc agacaatcag tgggtaaatc accgcccagg ccgcaatata gcgaccgcga
     1741 tgcgggagta ttttccagaa cattggtgcg atcgatacaa gcccaatcag taatgcgagg
     1801 ttaattcgcc agcgttggtc gtgtgggtaa agcccataca taaactgacc aaatcgttcg
     1861 tgaatgaaga cccaacaggc accggctttt gtgcagtcag cacgcgttga gccaacccaa
     1921 ttagcctgca aaaatgccca gttcagcaac ggtgggatca gttcccacat caaccatatg
     1981 cagccaatag tcagcaggct attgctccag ctggagaaca gattttttcg cacccacacc
     2041 atcgcgcggc ttgagttatg gctcgccggg cgcgagggct gagacagcaa tacttttgtc
     2101 atcatgactc cttagcgttc aacgatcgcg atgcggcggt tatagatatt catcagcagc
     2161 gagatagtca ggctgataat cagatagacc gacatggtca tggctatcgt ctcgatggct
     2221 tgccccgtct ggttcagcac ggtgccagca aacagcgaaa ccatatcggg atagccaata
     2281 gcggcggcaa gagaggagtt tttgacgatg ttgagatact ggctggttaa cggcggaata
     2341 attactcgca gtgcctgggg aataatgacc tggcgtagcg taaccgggtt gggtaacccc
     2401 agtgaccgag ccgcttcatg ttgaccataa ggcactgcct ggatcccagc gcggataatc
     2461 tcggcgataa atgcagaggt ataaaccgaa agtgccagcg ttaaggctgc cagttcagga
     2521 attaaaacca ttccgccgcg gaagttaaag ccttgtaggg ctgggacatc ccagtgtagt
     2581 gctgcgccaa aaagccattg cgccagtaaa ggcaaaccaa tgatcaacac tgcggcgatc
     2641 ggccaggagc ggcgcagttg tccggtcttt atctggtatg ttttattaaa gcggaatagc
     2701 ccaacagaaa ggactatagc cataacaaca gccaggataa acgcaataaa tccgtctccc
     2761 agctgcggtg acggaatata aagcccacga ttgctcaaaa aggccagatc aaacgcgctc
     2821 acagcttggc gtggtccggg caaattgcgc aacacggcaa agtaccagaa gaagatttgc
     2881 agcagcgggg gaatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     2941 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     3001 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     3061 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     3121 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt gtgaaataac
     3181 caaccaacaa tgccgacaac agcaacaacg gcaaggatct ggaataacca ggcacgaacc
     3241 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3301 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3361 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3421 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3481 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3541 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3601 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3661 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3721 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3781 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3841 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3901 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3961 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     4021 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     4081 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     4141 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4201 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4261 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4321 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4381 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4441 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4501 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
     4561 aaaatgaaac atgcgccaca ttttggcgca aaatatttac gcgtgtttta ctaagcagaa
     4621 taatgattgt tagcgtgagt atatttctgg tgggaagtgt gaaagttaaa ttgatgcgtt
     4681 atttcgaaag cagcgaaata acgcgatata acatgctaaa tcagcagaga attagccagc
     4741 agaatatagc aatattttat cttatgtaaa gatactgtat cgttaaacag agcgcgaaac
     4801 tccgcgccct gttcagttat ttcgacagag cgatgatccc catcgtcagg ccagcaacag
     4861 ccgctgcccc gccagcaata gcaccagccg tttcccagtt atcctgagtc gtgcctttca
     4921 tacaggtatt ggtgtgaacc agacgaccct gatcgtcata aaccggaacg cagggcgaat
     4981 cgtgagcaca gcccgccagc aatgcggtga gcaatgccac aggaattaat cttttcatgg
     5041 tgttacctca gtaataaacg ctaacgctta ataagttaac tctcttatta acgagctgaa
     5101 tgccatttta tcaccggagg ttataaatct tgcggaatga ataatgacaa attatgtgaa
     5161 ttgtaaaaat agtgaaggga gcgggaacta tagaaagctt ttaaagagtg agcagaagaa
     5221 tatcttatta atttttatgg gtttcactgg aaataataaa ggcacccgaa agcgccttta
     5281 tgtttctgat ttatccttta aagcaacggc gaatcaccac aaagaacacc ggtacgaaga
     5341 agattgccag caacgttgca gagaccattc ctcccattac cccgataccc acagcgttct
     5401 gcgcgccact gccggcaccg ttactgatag ctagcggtaa tacgccgaga ataaaggcga
     5461 gagaggtcat caggatagga cgcagacgca tacgtactgc catcagtgtc gcttcaacaa
     5521 cacctttacc ctctttctcc atgagatctt tagcgaactc aacaatcaaa atagcgtttt
     5581 tggccgacaa gccaattgtc gttagcaagc ccaccataaa gtagacgtca tttttttgat
     5641 taaagagtgt cgccgccagc agcacgccga caatccctaa cggcacgacc aacataaccg
     5701 agacaggaat tgaccagctt tcatagagtg cagcaaggca caggaaaaca accacaaagg
     5761 aaattgctac cagagcggga gcctggtttc ccgataagcg ttcctgatac gacatacccg
     5821 tccagtcata accaatgccc gcaggtaatt ttgacgcaag gttttccatc aacgccatgg
     5881 catcgccgga actggttcct ggcgcggctt ccccctgaat ctccattgac ggcagaccgt
     5941 tgtagcgttc cagtcgcgga gagccataca cccaatgtga agtggtaaag gcagagaatg
     6001 gcaccatttc gccgttggcg ctgcggacat aaagtttatc gacatcttct ggcagcatac
     6061 ggaatttggc atccgcctga acatacacct ttttcacgcg accacggtcg atgaagtcgt
     6121 taacgtaagt cccacccaac gccgttgaaa tggtctgatt gatgtcagaa agtgaaacac
     6181 ctaatgccgc gccttttcct ggtcaacttc cagtttaaac tgcgcggtgt cttccaggcc
     6241 attaggacgc acgctgacta agctggcagg atgttgcgcc gccataccaa gcaactggtt
     6301 acgggcctgg gttagggcat cgtgacccag cccagcctga tcaattaact caaagtcgaa
     6361 acccgttgcc gtgcccagtt caacaatggc tggcatattg aatggaatga caaaaccgtc
     6421 gcggatcttg cccaattcca ttttggcacg atggattacc gcttccgcac tgttttcatc
     6481 accattacgc tcttcccacg gtttcagact gacgaaggcc ataccggcgt tttgtgcctg
     6541 gccgctgaag ctaaagccgt taaccgtaaa gacactttca acgttcgctt tctcgttctt
     6601 cagatagtaa tccgtcactt gatccaacac tttttgcgtc cgctcttgcg tcgcgccagc
     6661 gggtaactga atcatggtca gaaagacacc ctgatcctct tcaggtaaga aggaagacgt
     6721 aaaaacaaca ccaccattcc tgcaacaatc agcgcataga tcagtaaata tcgtcctgtg
     6781 gaaccgagga ttttgccgac gctgttggtg tagtggttaa cgctatgatc gaaggtggta
     6841 ttaaaccagc cgaagaaacc gcccttattt tcgtgatgct cagcagagac gggtttaagc
     6901 agcgttgcac ataacgcagg ggtaagaatc aatgccacca gaacagaaag tgccattgcc
     6961 gaaacgatgg tgatagagaa ctggcgataa attgccccag tagaaccgcc gaagaatgcc
     7021 atcggaataa ataccgctga cagcaccatc gcgataccca ccagtgcgcc ctgaatttgc
     7081 gacatcgatt tttccgtcgc ttcttttggc gggagcttat cctccatcat cacgcgctcg
     7141 acgttctcca ccaccactat cgcatcatcg acgagcagcc cgatggcaag caccatcccg
     7201 aacatcgtta gtgtgttgat ggagtaacca aaagtggcga ggatggcaaa cgtccctaac
     7261 aacaccacgg gtaccgcaat ggtggggatc agcgttgctc gcatattctg caagaacaga
     7321 tacatcacca ggaacaccag cataatggct tcgaacagcg tttttaccac ttcgtgaata
     7381 gaaagctgga cgaatggcgt ggtgtcataa gggtagagaa ccttcattcc ctgcgggaag
     7441 aatggctgta attccgccag ttttgcctta atggctttcg cggtatcgag agcattcgcg
     7501 ccggttgcca gcttaatccc caggcccgcc gccggttttc cgttgatacg agcgataacg
     7561 ttatagtttt caccgccaag ttcaacccgt gcgacatctt tcaggcgtac caccgagccg
     7621 tcactgttta cgcgcagggt cactttgccg aattcttccg gattttttaa ccgcgtttga
     7681 gcaataatcg aggcattcaa ctgttgccct ggtaacgctg gcgttccgcc caactgtccg
     7741 gcagcgatct gatcgttctg taccttcaac tggttaatca catcaaccgg tgtcagttta
     7801 tatttgttta gcagatcggc gtccagccag atacgcatcg catactgtgc gccgaaaagc
     7861 tgtacgtcac cgacgccatt cagacggcta agcgtatctt taacgttaga ggccacatag
     7921 tccgagatat cgtcctgtgt ggtgtctggg ttatcagaga caaagcccgc caccatcaaa
     7981 tagctgctac tggacttttc aacactgatc ccctgctgct gaacctcctg cggcagcaac
     8041 ggcgtggcga gctggagttt gttctgcacc tgcacttgcg cgatatcagg atcggtcccg
     8101 gactggaagg taagggtaat tgtcacgcta ccggcggaat cgctggtgga ggacatatac
     8161 atcaggttat cgataccatt catattctgt tcgataacct gcgtcaccgt atcctgcacg
     8221 gtctgcgcat cagcacctgg atagtttgcc gaaacagaaa ccgcaggcgg tgcaatggtt
     8281 ggatacagag cgacgggcaa ttgtaggatc gccagatttc agaccttcgc taatcaacca
     8341 tttatcgcca atcgcctgac tggcaacgac agggcgcgct tccacctgac ttttatcgtt
     8401 aacaatcagc acggttgcat caccacgcgg tgtgcggcta acgccttgtt gcgggataag
     8461 aatggcgtca ggttggacgc cttcatcaat ccgtgcacgc acaaacatac ccggcaaaag
     8521 cgtatgttgc gggttaggga agacagcacg tagggttatg gagccggtgc tttcatcaac
     8581 ggtcatatca gagaattgca gcgtaccttt caggggatag gtttgaccgt tttccatgac
     8641 caactctacg ttgctggtgg cgttttcctt atgcaaattt ccttgctcta cggattgctt
     8701 cagcctcata aaatcgttgc tggattgggt cacatcaacg tagataggat agagctgctg
     8761 gacagtcgcc agttcagtcg tttgcccatt agtgacaaga gcgccttcgg tcacggtcga
     8821 tttgccgata cgtccgctaa ttggcgcagt cactttggta taagcgagat tgatgcgagc
     8881 gctttcgact gtggcttttg cggcaatcac ggcggcatcg gcctggcgag catcagcaat
     8941 ggcctggtcg tactcctgct ggctgatgta tttcgtaccc acgagcggaa cgtaacgttt
     9001 taccgtcaaa tgcgcgatgg cggcggcggc ttcacttttc gccagttcgc ctttcgcgct
     9061 gtcataattt gcctgatagg tcgcgggatc gatctggtat agggactggc ctgcttgtac
     9121 atcgctgcct tcagtgaaat tacgattcag tacgatcccg ctaacctgtg ggcgaacttc
     9181 ggctatacgg taagcattgg tgcggcctgg taattcagtc ttaacttcta acggggccgt
     9241 tttcacaata tgaacggtaa cctgcggttc accgacgtga gctttctctt ctcccttatc
     9301 gttacaaccg gcgattaaag ccgcggagat cagaataaag gagggcagga ggaaaaacct
     9361 ggcatgtttc gtcattacta ttcctcaaaa aaccaaaagc gcgttattta cccagaaagg
     9421 caacgcgtta
//
LOCUS       DADLSB010000147         9295 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.147, whole genome shotgun sequence.
ACCESSION   DADLSB010000147 DADLSB010000000
VERSION     DADLSB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.147"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(68..256)
                     /locus_tag="JN331_003304"
     CDS             complement(68..256)
                     /locus_tag="JN331_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HAY7261366.1"
                     /translation="MYKITATIEKEGGSPTSWTRYSKTKLTKSECEKMLSGEKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            complement(307..651)
                     /locus_tag="JN331_003305"
     CDS             complement(307..651)
                     /locus_tag="JN331_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261367.1"
                     /translation="MDTSRDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGGT
                     SNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDPQTQNE
                     TQQAGYDENIPV"
     gene            complement(791..2212)
                     /locus_tag="JN331_003306"
                     /pseudo
     CDS             complement(791..2212)
                     /locus_tag="JN331_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            complement(2236..2532)
                     /locus_tag="JN331_003307"
     CDS             complement(2236..2532)
                     /locus_tag="JN331_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HAY7261368.1"
                     /translation="MSIIECEIELDVINDSLAEFKCGNTLHSFIQISQKENVTVIIDD
                     GYVLGTFDCCVCAIKEIALLTARIEEADKKYGISYQRLKELSHIASSVRFFYAI"
     gene            complement(2607..2894)
                     /locus_tag="JN331_003308"
     CDS             complement(2607..2894)
                     /locus_tag="JN331_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261369.1"
                     /translation="MDMNPMLDYCFTPALRQTISHTGIKKWLWRIRKILQQKGDPDLI
                     QCDYHLCNKQFLPEKAFTGELIYMHGNMIARKKRKYCGAACAEKDKMAHEL"
     gene            complement(2905..3744)
                     /locus_tag="JN331_003309"
     CDS             complement(2905..3744)
                     /locus_tag="JN331_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7261370.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESLECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLL
                     SLK"
     gene            complement(4166..4318)
                     /locus_tag="JN331_003310"
     CDS             complement(4166..4318)
                     /locus_tag="JN331_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAY7261371.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
     gene            complement(4511..4918)
                     /locus_tag="JN331_003311"
     CDS             complement(4511..4918)
                     /locus_tag="JN331_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7261372.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNDLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAGVLQCSPTWILFGDEDKTPDPPVALNSALDLSEDELEMLRLYRALPKS
                     EQQAQISELRARVENFNRLFTELLEARKRNKHQ"
     gene            4996..5223
                     /locus_tag="JN331_003312"
     CDS             4996..5223
                     /locus_tag="JN331_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7261373.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWGELVPEGRAMRLQK
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            5207..5629
                     /locus_tag="JN331_003313"
     CDS             5207..5629
                     /locus_tag="JN331_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261374.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGASDDSVVVH"
     gene            5720..6475
                     /locus_tag="JN331_003314"
     CDS             5720..6475
                     /locus_tag="JN331_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016060759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7261375.1"
                     /translation="MSMKLMAKAMEIKVGNPLRKLVLIKLADNASDQGECWPSYQYIA
                     DQCEISKRSVINHIAALCESGLVKKITRKGEKGNTSNIYLLHLDGAENLPGNSANNSL
                     HCASDASGSAGVAPVASAGVAPRTSHSFEPVNEPDVGGSADADPQVSHRAKNKIDWQR
                     VLDSYHEILPEMPSVKILTDTRKKNLRTFWQKFGFDQQRWESYLRYVAENCRWMLEDR
                     PNGSGGFWKRKNLDYLITERCYVAVKEARANDR"
     gene            6465..7868
                     /locus_tag="JN331_003315"
     CDS             6465..7868
                     /locus_tag="JN331_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7261376.1"
                     /translation="MTDNTFPVPYRVDAEQAVLGCLMVNTDTERAGLVYSMLKPESFY
                     VAAHRVIFREIRGLFRAGKPTDLLSLANVIEAKKLDAETGGFAYLAEISRSATLSAMV
                     HYAGIVREKSILRYAVEKLHACIGIMNQPTDTSATERLGAVQQVIGTVAEYARTGKTG
                     GLRPVSDVVNDWIDDLERRFSSPENAAGLTLGISSLDRLMAPKHVLRGSLVVIGARPK
                     MGKSASLNRIVTHFALNHRLPTLVFTLEMTDRSLVERMVAQEARVNSEIFYVGASDDS
                     DMARAMAKAAELAESNLMIDSTPGVTLAHVIAECRKVKRQRGVVGLVAIDYLTLMKAE
                     AAERRDIAFGDITTGLKNLAKELDCVVLLLTQLNRKLEDRADKRPTPSDSRDTGQIEQ
                     DCDVWIGLYRDAVYNRNADSQLMELILRLNREGATGTAYALMDNGSVTDVSAEDAARR
                     LRLGGPARKPYAQTEDF"
     gene            8035..8796
                     /locus_tag="JN331_003316"
     CDS             8035..8796
                     /locus_tag="JN331_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HAY7261377.1"
                     /translation="METVFDALKAMGKATSTELAARLDISREEVLNELWELKKAGFVD
                     KSAYTRRVADNNVQQEQPAQAELPEETTTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQGGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPLFTETRADDLVLPSLHMANRELRRAKSH
                     VQKWECVCAALRELNKHRDIVRQITDISRRVASEK"
     gene            8812..9204
                     /locus_tag="JN331_003317"
     CDS             8812..9204
                     /locus_tag="JN331_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY7261378.1"
                     /translation="MAKVFTPEEREEVKARIVELVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSDCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYDRRQN
                     IICRECRKSEVMQRVLAFYQGNFQEVMA"
     gene            9201..>9295
                     /locus_tag="JN331_003318"
     CDS             9201..>9295
                     /locus_tag="JN331_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HAY7261379.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKR"
BASE COUNT         2364 a         2075 c         2403 g         2453 t
ORIGIN      
        1 gaatactggc gggtgtggtt gatttttaat aatcagctat gaagttctaa tctggatata
       61 atgaaattca cgaggactga agtttctcgc aattaaaatt tatcagtttt actttctgct
      121 ctctggaaac gcctgcttct ttttcccctg agagcatttt ttcgcattct gatttcgtta
      181 gttttgtctt tgagtatctt gtccagctgg taggagatcc accttccttt tcaatagtgg
      241 ctgtaatttt atacatgaac acctccatta atattttcaa aaaccgtgtc caaaatgcac
      301 ttggttttac accggaatat tctcatcgta tcctgcttgt tgcgtttcgt tttgtgtttg
      361 tggatccggg aatgcatatt caacgccttc cagttcaatc catagtgcat tacgaccagc
      421 tttgattgtc ggccagtcca tacctttgat tctttcccat gagcgggagg tgaatgtttt
      481 ttccagcaaa tcagcttttg cgcgtttggc gtcgttactg gtgcccccat ggtgcttgtt
      541 gagcaactcg ataatttcat caagtgcgat ttcttttgca cgcttttctt ttagccatgt
      601 cggtgtaccg tcgttggcaa ataattcact gttatcccgt gaggtatcga tgccgaggtg
      661 ttcacctccc aggttaagaa actcgatatg aggaagaaag tttttaaatt ttgacttcat
      721 ttgaggagat atgatgaccg ggaatccatt tcgaatcatt cgggtcgcta atcccttcaa
      781 caaattctcc gcgagaggca gccagtaatt tgtctgcatc gacaggattt ttgggcggaa
      841 tgtttttccg ggcttcatgg agttctgtcc gcagtttctg atatttcgca tcaacagcat
      901 ttacctgtga ctgagcatcc agcggctgcg tgtcctgatg atgttcagtt gcatccggtt
      961 ccactgtttc agccgttgcc tgttcatctg ccattgcgca agatggttgc ggtttttctt
     1021 catcatcctg ttttccttct tctgttacac gttgcggcat cggggcagag gagcgaccgc
     1081 aggcaatatc cacgatttcc ggatcagggt tggcatgatc ggtttcagtc agtaccttgt
     1141 tcagatattc agtgacgtgc gcggggatga cctcgatacc aatcggtgca tctttcactg
     1201 acgcaaccac gatggcgcgg gaataatcca tcccgccagg catggtgatg aatttgtcgc
     1261 ggaaaacaga aaagggcggt ttattttcag cgataatttc ctcgacacgt ttagcgtgtg
     1321 ccggatgaag gttataaatg tccacgtcca ttgaacgagc cagtacgcca gtggctacgt
     1381 cgcgcgccag tgacgtcaga tcgtgaacga aaccttcgcc gcgatcggtg aggttcccgc
     1441 cgccagcatt agcaccggaa gccgtgcgag tgatacgcga aacacgattt cctttcatcc
     1501 actcttttgt cagcagaccg cgatcggtgt agttggcatc cagatatgct tcgaaaaaag
     1561 cagtcatcag tcccagattt gaattgccag gattagggaa aactttgtca gtatcacgca
     1621 cgagtttgtg gaggtcgcga atctccagcg ggtcgagcag actggtttta tgcgaaacag
     1681 ccagagcagt aacagccggt agttcttcat cccgagcaat gtgtaatgcc tggagttcgt
     1741 cgcgtgaaac gtgcgttacc ggtttttcgc tgccgtgttg cgcaagccaa cgaatgggca
     1801 gttcctgacc ggaaactggc aggagcatgt tctcctcaat ttctgtcatg tcttcgccgt
     1861 tgacgttggt attgtcagtg ctggctggtt tgtcctgaac agagggagaa gatgcgataa
     1921 ataccattgt gatgccatct tccccgcctt tttcgtaacg gttgcagaat tccgtatcaa
     1981 acacgccttc tggtgggagg tcatcaacaa cgggcaaatt gacgcgaaca ggttttttaa
     2041 agtcatcttc atcgtagcca gcatcgtcaa tcgcaacagc accacgggag atggcaatgg
     2101 ataatttttt cgcttcagcc cagtaaaaac cgcctttaat accgaggcgt tttcttactt
     2161 tgtcattttt tgcttcgtaa tacagtgggt aaacttgttt atcggtgctc attgtttttt
     2221 aacctcaact catattcaga ttgcataaaa gaaacgaact gatgatgcga tatgagagag
     2281 ttctttcagt cgttgataac tgataccgta tttcttgtca gcctcttcaa tgcgagctgt
     2341 gagtaatgca atttctttaa tggcacatac acaacaatca aatgttccca gaacgtaacc
     2401 gtcatcaata attaccgtga cgttttcttt ttgggaaatc tgaatgaaac tatgtaatgt
     2461 attcccacat ttaaattcgg cgagagaatc gttaataaca tcaagttcaa tttcacattc
     2521 aataatgctc attgctgttt ccttttttaa ggttgagtga atccctgcca ttgctggcat
     2581 aaattcagtt tcgaatattc agttaattaa agttcgtgtg ccatcttgtc tttttcggca
     2641 caagctgcac cgcaatattt tcgtttcttt ctggcaatca tgtttccatg catataaata
     2701 agctcgccag taaaagcttt ttcaggtaaa aactgtttat tgcagagatg gtaatcacat
     2761 tgtattaaat ccgggtcacc tttttgctgg agaatttttc tgattctcca cagccatttt
     2821 ttaatcccgg tatgactgat ggtctgcctg agggcaggtg taaaacaata atcaagcatg
     2881 gggttcatat ccatagctcc atttttattt taaggaaagc aactgctcta cggtcatgtt
     2941 tttaattgcg ccccggttta caagagtcca gccctgtttt tccagataaa accggaaagt
     3001 ctccagggta cagaccagtg cgccatcagg aacggtttcg gtgaattcga cattgccgaa
     3061 tttgtcgaag tgaacaacca gagtgcgacc atcacccgga atcatcttgt cagcaggtgg
     3121 ggtgttattc tggcgcagtt cggcctccat gcggtcgaac tcagcaatgt aggcttcctt
     3181 gaatgtggcg gcttttttgc ccgtgaagcc catcacaagg aaaacgaagc cgtttttggt
     3241 gatttggtag gcgttataag aattgccgcg gtgttcgaat ttaacccgcg aaaagttgct
     3301 ggttaaaaat agttcagaac attcgaggga ttctattttt tgaacaacat ggtgatgctg
     3361 cttgccaaaa aactcagcaa tcgaaataga agtagtgacg gcgcgaccat tttcgatggt
     3421 tacgtcaggg tgagaaaggg tagtagccat aatgacatcc tcaagtgata agttaattaa
     3481 ctcaccagca gaggtgctaa tctcatgggt ggtgagacgc acagggttag cactaccggt
     3541 cactcgagaa cccggccagc cttgcggctg ccccgcacgc ctcaccataa tttgaatgtg
     3601 gctgtgcatt acgcataaaa aaaccgcttc agcgcggtta tgcgctcgag taactttcgg
     3661 ggtgctaatc ccggcacccg ttttatgagg tgcaggtgca ctataattcc acccgttctg
     3721 gttttcaata gctacattca acattttctc tttcctttca tcaccgaagt gaattttgtg
     3781 atgcggtgcc tggtgcatcc aggtgacgtt aaccagttaa caattacagt cggctttccc
     3841 acccaaaaca ataaggacta acatgacttt taactgtgcc gcgtgcgctt agccgcattc
     3901 accgcatcac aaaattcact ttaaaaaggg cggacatcag ccagcaatta aaccgatgcc
     3961 gccaactggt acttcacaca gcaatgtcgt tatttacaac cggaagcgca ctcccaccat
     4021 ttaaatttca cagacaagac cgactcttta tgaataccgg aaatgcgcct tcgtgttgtg
     4081 cccggtttta tttcaccacc tccgggcttt ggtggcctcg gctatacctc tacagcgaga
     4141 atattgaatt aatccaataa atggattagc tggtattttt ggcaagccag cgacgtgcgc
     4201 cagcttcggt tttaaacgtt ttgcttttgg tatacgtcat ggcggtgaat gtgccgtcct
     4261 gattgggaaa cacgccacac actagggatt cgttgttgcc aagatcgata gtatccatgc
     4321 tgacctcatt tccccttaac gccggggtgg cggaactgtt tactgagagc accgtgcggt
     4381 gtcttgatag atgttaattt agttttctca tgataattga tcaagtattt ttgatgagaa
     4441 atctcaatat ttaatgcaaa ataaagccaa tacattgaaa tgtaaggctt taaaatttgt
     4501 gaaggggggg ttattgatgt ttgttacgtt tgcgagcttc tagtagctcg gtgaataggc
     4561 gattaaaatt ctcaacgcgg gcacggagtt cgctgatttg tgcttgctgc tctgattttg
     4621 gaagtgcgcg atacaatcgc aacatctcca actcatcttc cgataagtct aaggcgctgt
     4681 tgagtgcaac tggtggatct ggtgttttat cctcgtcacc aaacagtatc caagttggtg
     4741 aacattgcaa taccccagcc aggcgatgca aattttgccc gcgcggggct gtatggtcgc
     4801 tttcccatag tgaaattgat gagccagata cgccagcagc tttgcttaaa tcgttttgac
     4861 ttaaaccaac ctgtttgcgt ctttctctaa ttcgttgacc taaagttttc tcgttcatat
     4921 ttagatatct taataaccct tgacttgaga ttccttgagt gattactttt gagaaaactc
     4981 aactttggag gggtgatgtt taaatcagac gtaattaatt tttatgggac gaaagccaaa
     5041 gtagcgaaag ctgctggtgt tgatccatct gctgtttctc aatggggtga actggttccc
     5101 gaaggtcgcg cgatgcgcct acaaaaagca tctggcgggg agcttcagta cgaccccaaa
     5161 gtttatgacg aatatcgtaa ggcaaaacga cctgggaagg tgattcatga aaatcaggca
     5221 tgagcacatc gaatcagtgc tgttagccct ggcagccgaa aaagggcagg cgtgggtcgc
     5281 taacgcaatt actgaagaat atctgcgcca ggggggcggc gaattgcccc tggtaccagg
     5341 caaggactgg aacaatcagc agaatatcta tcaccgttgg ttgaaaggtg aaacgaaagc
     5401 gcaaagggaa aaaattcaga aactgatccc tgcggttctg gcaattcttc cgcgcgagct
     5461 gcgtcaccga ctctgtatct tcgataccct ggaacgccgt gcattactgg cggcgcagga
     5521 agcgttgagt acggcaattg atgcgcatga tgacgcagtc caggccgttt accgtaaagc
     5581 gcatttcagc ggtggtgggg cgtccgacga ttctgtcgtt gtgcattgat gtttatgccg
     5641 aacctcctct ggttctgttg attggggaat cacagattat atccggagga aggttcggca
     5701 ccagacgagg tttctatata tgagcatgaa actaatggca aaagcaatgg aaattaaagt
     5761 gggtaatcca ttgcgtaagc tggttctgat taagctggct gataatgcca gcgatcaggg
     5821 cgagtgctgg cccagctacc agtatatcgc tgaccagtgc gagattagca agcgttctgt
     5881 gattaatcat attgcggcgc tttgtgagtc cgggctggta aaaaaaatca cccggaaagg
     5941 tgaaaaaggt aatacaagta atatctatct ccttcatctg gatggtgcag aaaatttacc
     6001 ggggaatagt gcaaataatt cgctacattg tgcatcagat gcatcgggta gcgcaggagt
     6061 tgcacctgtt gccagtgcag gagttgcacc cagaaccagt cactcttttg aaccagtcaa
     6121 tgaacctgat gttggtggat ctgctgacgc agatccacag gtcagtcatc gcgcaaaaaa
     6181 caaaatcgac tggcagaggg ttctggacag ctatcacgaa atcctgcctg aaatgccatc
     6241 agtgaaaatt ctgacggata ccaggaaaaa aaatctgcgg acgttctggc agaaattcgg
     6301 ttttgaccag cagcgatggg agtcctatct gcgatacgtt gctgagaact gtcgctggat
     6361 gctggaagat cgtcccaatg gcagcggtgg gttctggaag cgtaaaaacc tggattacct
     6421 gatcaccgaa cggtgctatg tcgctgtcaa agaggcgcgg gcaaatgaca gataacacct
     6481 ttccggtgcc ataccgggtc gatgccgaac aggcagtgct ggggtgcctg atggtaaaca
     6541 ccgacactga gcgtgcgggg ctggtgtatt cgatgctgaa accggagtcg ttttacgttg
     6601 cggcgcacag ggttattttc cgtgaaattc gcggactttt ccgtgccgga aagcctacgg
     6661 acctgctcag ccttgcgaac gtgatcgagg cgaaaaaact ggatgctgag actggtggct
     6721 ttgcgtacct tgctgaaatc agcaggagtg ccacgctatc ggcgatggtt cactacgccg
     6781 ggattgtgcg ggaaaaatcc atcctgcggt acgcggtgga aaaattacat gcgtgcatcg
     6841 ggatcatgaa tcagccgaca gacacgagtg cgacagaacg acttggcgca gttcaacagg
     6901 tcattggcac ggtggcagag tatgccagaa caggaaaaac aggcggtctg cgcccggtaa
     6961 gtgatgtggt taacgactgg attgatgatc ttgagcgtcg cttttccagc ccggagaacg
     7021 cagcgggtct gacgctgggg attagctctc tggatcggct tatggccccc aaacatgtgc
     7081 tacgtggttc gctggtagtg attggtgcaa ggccgaaaat gggtaaaagc gcctcgctga
     7141 acaggattgt gacgcatttt gctctgaatc atcgtctgcc cacactggta ttcaccctgg
     7201 aaatgacaga tcgcagcctg gttgagcgca tggttgcgca ggaggcgagg gtaaattctg
     7261 aaatttttta tgtcggtgcc agtgatgact cggatatggc gcgggcgatg gcgaaagccg
     7321 cagaactggc agaatcaaac ctgatgattg acagcacacc aggggtaacg ctggcccatg
     7381 tgatcgctga atgccggaag gttaagcgtc agcgtggtgt tgttggcctt gtggcgatcg
     7441 attacctgac gctgatgaag gcggaagccg cggagcgtcg tgatattgct ttcggggata
     7501 tcacaacggg cttaaaaaat ctcgctaaag agctggactg cgtggtttta cttctgacgc
     7561 aacttaaccg aaaactcgag gacagggcag ataaacgccc cacaccaagc gacagtcgcg
     7621 acaccgggca gattgaacag gattgcgatg tgtggattgg gttgtaccgg gatgctgtct
     7681 acaaccgtaa cgcagactct cagcttatgg agctgattct ccgtctgaac agggagggag
     7741 caacaggaac agcatacgcc ctgatggata acggttcagt gactgatgtc agcgcagagg
     7801 atgctgccag acgtctgaga cttggtggtc cggcacgtaa accctacgca cagacggagg
     7861 atttctgatg cagtcacttt gtgatgttct ggaaaaaacg ctgcttcagt tcgggcgggg
     7921 ggaaacggcg gagcagaaaa cacgaaacca gaagaatctg gttcatctga gaaaaatgat
     7981 tgatgcagcc aggctggctg aaaagctgga aaaacaaaac cggaggtaat tttaatggaa
     8041 actgtttttg acgcactgaa agcaatggga aaagccacat ccacagaact tgctgcgcga
     8101 cttgatatca gtcgtgaaga agtgctgaac gaactatggg aactgaaaaa ggctggtttt
     8161 gttgataaaa gcgcgtacac ccggcgtgtg gctgataaca acgttcagca ggaacagcca
     8221 gcgcaggcag aactgccgga agaaaccacc acagcaacag tagcgaaaat ctcagagtgc
     8281 gatttaaccg cgacgattga acaacgcgga ccacaaacgg ctgatgagct ggctacattg
     8341 tttggtacca catcacgcaa agtggcttca acgctggcaa tggcaatcag caaaggtcgt
     8401 ctgattcgcg taaatcaggg cggtaaattt cgttactgca taccgggcga taatttacca
     8461 gcagagccga aagcagcatc ggtagcggaa actgatggta aggcctttcc tcagcccgca
     8521 ggtgttgcat taccagtaca ggaggctgca acacaggaag atattaaaac agaaacggtg
     8581 gcggacattg tgcagtcgct gccattgttc accgaaacgc gagcggatga cctggtttta
     8641 ccatcgctgc atatggcaaa ccgcgaactg cgtcgggcga aaagtcatgt ccagaagtgg
     8701 gagtgtgtct gcgccgcgct gcgggagctg aacaagcacc gggatattgt tcgacagatt
     8761 actgatattt ctcgccgtgt tgcatcggaa aagtgattgc cggaggcacc tatggcaaaa
     8821 gtatttacac cagaagagcg ggaagaagtg aaggcgcgca ttgtggaact cgtgcgcctg
     8881 agcggacgag aaacttttcg acaactggca gataaaacgg gtgtcagtaa gaccgctatt
     8941 cgtcgtttat ctggtgcgct tgcggccagt ggtgatgtct ggctctctga ttgcggggta
     9001 tttccatcag agcaggcgta tcgcgtatgg cgtaagacac cggagaaggc tgctgacccg
     9061 acactgattc gaaagttacc tgacggagaa atacgccgct acgacaggcg tcagaacata
     9121 atctgtcgcg agtgccggaa gagtgaggtt atgcagcgag tgctggcgtt ttatcagggt
     9181 aattttcagg aggtgatggc gtgagggtga gagtctatat cgccggtcca atgaccgggt
     9241 ataaaaattt caaccgtgag gcgtttcaca atgcggaaga ggaactgaaa cggga
//
LOCUS       DADLSB010000148         9183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.148, whole genome shotgun sequence.
ACCESSION   DADLSB010000148 DADLSB010000000
VERSION     DADLSB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.148"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..393)
                     /locus_tag="JN331_003319"
     CDS             complement(<1..393)
                     /locus_tag="JN331_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY7261380.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAG"
     gene            complement(415..741)
                     /locus_tag="JN331_003320"
     CDS             complement(415..741)
                     /locus_tag="JN331_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY7261381.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(960..1925)
                     /gene="glk"
                     /locus_tag="JN331_003321"
     CDS             complement(960..1925)
                     /gene="glk"
                     /locus_tag="JN331_003321"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY7261382.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            2129..3385
                     /locus_tag="JN331_003322"
     CDS             2129..3385
                     /locus_tag="JN331_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY7261383.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            3500..3826
                     /locus_tag="JN331_003323"
     CDS             3500..3826
                     /locus_tag="JN331_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7261384.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(3967..5205)
                     /gene="mntH"
                     /locus_tag="JN331_003324"
     CDS             complement(3967..5205)
                     /gene="mntH"
                     /locus_tag="JN331_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY7261385.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            5541..6743
                     /gene="nupC"
                     /locus_tag="JN331_003325"
     CDS             5541..6743
                     /gene="nupC"
                     /locus_tag="JN331_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY7261386.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(6793..8982)
                     /locus_tag="JN331_003326"
     CDS             complement(6793..8982)
                     /locus_tag="JN331_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HAY7261387.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGNLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
BASE COUNT         2373 a         2405 c         2197 g         2208 t
ORIGIN      
        1 accagccggg aacgccagtt tgccaccgat agagttcgcc acaaatgcag caaacatcgg
       61 aatggcaaaa ccaaacaaca cgccgccgaa ggactgcgac agccaggcaa atttaagtaa
      121 ggagaggtcg aaaccggaga atttcccgct attaagagca tccatgatgc cgatctcagc
      181 aggaatttta agccagctat aagcaatcag ctggctgaaa gcgaggatca caccgcccat
      241 aattaaggtc ggcaccatgc gtgaaatccc tgacatcaca tgctgcggga gttcacccca
      301 gaagctgctt ttagaggcct gcggattctt aactgccgct gccgcaccgg atgcgccagg
      361 cacaacggtt gcactgcgtt ttttaatggc cataacaatt atccctgtcg aatattactg
      421 ttgttcagag gcaatcatct cttcgatttc tttgatgatg cccgcagcgt ttttaattgc
      481 gtcctgcaaa gtgatttcat aaacgtcgcg tgattcgaaa cgttcgttat cttccggggt
      541 aactgccacg gaatggatga tgatggtcgc ttcggcgata tcctgcgccg tcaggcgatt
      601 ctggatgccg tccgcgccct gggtttcaat tttcacttca taaccggctt ctaccgccgc
      661 ttcttccagc gcctgagcgg ccataaaagt gtgagccagg cccattgggc aggcacataa
      721 ggcaatcagt ttcttactca tcgttaattc ctcgttcagt gatagttgac gagtgaaatt
      781 atgaacgtgc aaaacaaatc gccgttacca gacaaataat gcatttactg gagaattaat
      841 cccgctgtac ggaagtgtga cgcaggtcgg ccttgtgagg ggacacaaag aaaaattgaa
      901 ttaactgtat gatttaaaag attatcggga gagttacctc ccgatataac aggaaggatt
      961 tacagaatgt gacctaaggt ctggcgtaaa tgtgcaccgg aaccgagaag acccggatag
     1021 tcatggacga tgagatacac cggaatatca tggacatatt ctttaaagcg ccctttatct
     1081 tcaaatgcgg cacggaaacc ggaggctttg aagaactcaa ggaagcgcgg cacgataccg
     1141 cccgcaataa acacgccgcc aaatgtcccg agattgagcg ccagattgcc gccaaaacgg
     1201 cccataatga cgcaaaacag cgacaatgcg cggcggcaat cggtgcagct gtcagccagc
     1261 gcgcgttcgg taatatcttt tggcttgaga ttttctggca ggcggttgtc agctttcaca
     1321 attgcgcgat acaaattcac cagcccaggg ccagaaagca cgcgctccgc cgaaacatga
     1381 ccaatttccg cacgcaatat ttcgaggata atggcctctt cttcactatt cggcgcaaaa
     1441 tcaacgtgac cgccttcgcc cggcaagctt acccaacgct tatcgacatg gaccagatgc
     1501 gcaaccccaa gccccgttcc ggcaccgtaa accgcaatag gcttaccttc gaccggttct
     1561 gcgccaccaa actgaatcag atgctctttt ttcagcatcg ggatcgccat cgatacagcg
     1621 gtaaaatcgt taataatttc cagatggcta aaaccgagat tctttttcat ttcggcaatt
     1681 gagaacgccc aggtatggtt ggtcattgcc acccagtcac cggtaattgg gcaagcgatg
     1741 gcaatacagc cgtctttcac ctcgacctta tgttcttcaa gataaacgcg aatgaccgct
     1801 tcgaggctgg ggtaatcaag ccctgaatag gtcttagcct gcgagatttc accactggca
     1861 atatcacaca gagcaagacg tgcgttggtg ccgcccacat caccgactaa tgcatacttt
     1921 gtcattcttc aactgctccg ctaaagtcaa aataattctt tctcacactg taaatacctg
     1981 ggggcataac aacaacgccg gaaaggcagg ctccctgtaa atatcgatct gggtcacaca
     2041 attactttat cgtttcagca ccaattgcag cgatgccttt ttgcaagctg ggcaaactaa
     2101 gtatctgacc ccgcataagg aatagaacat gctccatccg cgagccagaa ccatgttgtt
     2161 attatcgctc cccgccgtgg caattgggat tgcgtccagt cttattctga ttgtggtgat
     2221 gaaaatcgcc tcggtattac agaatttgct ctggcaacga ctgccgggaa ctctggggat
     2281 agcccaggat tcacccctct ggatcatcgg tgtattaacg ctaacgggta ttgcggtggg
     2341 gttggttatc cgtttcagcc agggtcatgc cggaccagac cccgcctgtg aaccgctgat
     2401 cggcgcaccg gttccgccct ctgcgctacc tggacttatc gtagcattaa ttctcggtct
     2461 tgctggcggc gtcagcctgg ggccggaaca tccgatcatg accgtcaata tcgcccttgc
     2521 cgtcgccatt ggcgctcgtc tgttaccgcg cgtcaaccga atggagtgga ctattttagc
     2581 ctctgccgga accatcggcg cgctgtttgg tacacctgtt gcggcggcgt tgatattttc
     2641 gcaaacctta aatggcagta gtgaagttcc gctatgggat cgtctttttg cgccgttaat
     2701 ggcggcagca gctggtgcac ttactaccgg attatttttc catccccatt tttcactgcc
     2761 cattgcgcat tacggacaga tggaaatgac cgatattctc agcggtgcaa ttgtcgcggc
     2821 gattgccatc gcagcaggga tggtcgccgt atggtgctta ccacggttgc acgcgatgat
     2881 gaatcaaatg aaaaatccgg tgctcgtgct gggtattggc ggatttattc tcggaattct
     2941 gggggttatt ggtggaccag tttcgctgtt taaagggctg gatgagatgc agcagatggt
     3001 ggcaaatcag gctttcagca ccagcgatta ctttttgctg gcggtaatta aacttgccgc
     3061 cctggtcgtt gctgccgcca gtggctttcg cggtgggcga atcttcccgg cagtgtttgt
     3121 cggcgtggca ttagggttga tgctgcatga gcacgttccc gccgttccag cggcaataac
     3181 cgtttcctgc gctattctcg gcatcgtgct ggtggtaaca cgcgatggct ggttaagtct
     3241 ttttatggcg gcagtcgttg tacccaatac cacattgcta ccgctgctct gtatcgtcat
     3301 gcttccggca tggctgttat tagcaggtaa gccgatgatg atggtcaatc gtccgaagca
     3361 acagccaccc cacgataatg tttagcaaaa aatgctctcc tttatgattt aagagttatg
     3421 gcgatgatac ggggctttac ctccccgtaa tattgcgtta acaggccgct gacggcttcg
     3481 atcgtgaagg agaataacaa tgttcaggtc actgtttctg gcggccgccc tgatggcatt
     3541 taccccgctt gcagcaaatg caggtgaaat caccctactg ccatcgatca aattacaaat
     3601 tggcgatcgc gatcattacg gtaattactg ggacggtggt cactggcgag atcgtgatta
     3661 ctggcatcgc aattatgaat ggcgcaaaaa ccgctggtgg cgtcatgata atggctatca
     3721 tcgtggctgg gataagcgta aagcatatga gcgtggctat cgtgaaggct ggcgcgatcg
     3781 tgacgatcat cgcggaaaag gccgcggaca tgggcaccgc cattaaacgc gtcctccaat
     3841 ggagcacaat gcctgatgcg acgctagcgc gtcttatcag gcctacaaac cggctcttta
     3901 acgcgtaggg cggataaggc gtttacgccg catccgccaa tagtgccaga tgcgacgctc
     3961 attcaactac aatcccagcg ccgtccccac caacaaccag atattcagcg ccacgaccag
     4021 cactacaatc acccagcctg tctgttttac gcgtttgctg ttcaccagat cgcccatcaa
     4081 cttgctgtca ctggtgaaaa tcagcagagg aaccagcgcc agggcgatac caaaacttaa
     4141 cagcacctga ctcataacca gaatccgtgt cggatctaat cccatcagaa tgacaataaa
     4201 tgacggcaac atggtgactg tacgacgcac ccacagcggg atatggaagc gaatgaatcc
     4261 ctgcatcacc acctgccccg ccagtgtccc caccaccgtt gaggacagtc cggcagcaac
     4321 cagacttaac ccaaagaccg ttgccgcagc atggcttaac agcggttgca gcgtcagata
     4381 agcctcatca agatcggcaa caccagtatg accagaaaag tggaacgccg ccgcagctgt
     4441 agccatcatc gccagattga caaaaccggc aatcgtcatg gcgatagcca catcccattt
     4501 ggtggcggaa taacgttgtt gacgcgaacc gccatgtaaa tgctgagtga gcgaagagtg
     4561 caaataaatc acatgcggca taatcgtcgc ccctaacacg cctgctgcca ggaagaccgc
     4621 ctccgaagta ggtaaactcg ggatcaccat tcctttaccc agctgcgcca ggttaggctg
     4681 ggagaaaatc aactcgacaa tgtaagccgc ggcaacaaac aacagtaacc cgccaatcac
     4741 tttctccagc ggtttttgcc cgcgacgttg cagcattaaa atcaggaaag tcgcgatccc
     4801 cgtcagcacc gcgccctgca acaacgaaac accaagaatg agtttaaaac cgatcgccgc
     4861 accaataaat tccgccaggt cggttgccat cgcaataatt tctgcctgaa cccaatagaa
     4921 ccacactacg ggacgcggat agtgatcacg aatctgctcc gccagatttt taccggtggc
     4981 aatccctagt ttggcagaga ggatctgaat cagcatcgcc atcaggttgg cccaaacgac
     5041 aacccacagt agctgatagc cgaagctagc acccgcctga atattggtcg caaagttacc
     5101 gggatcgata taaccaatcg ccgcaatgaa cgcaggtccc attaatgcga gcctcatctt
     5161 gcgcgccgcc cgtccgctgc tactctcaac gcgatagttc gtcatcttgt gcctctaaaa
     5221 catagccttt gctatgtttc atgccatgcc aaacgagaat gattatcaaa ttcatttaaa
     5281 tggattgtgg tgatttctct gatagaccag gattatgatt acgaaaagat tgatgacttg
     5341 aatgttatgg ttatgtttaa tgttagcaca tttacataac tttcagcttc catacacaac
     5401 atagcagaaa tgtatgacag atcactattt ttgaagcctg tcacaggacg tcattatagt
     5461 gtgtgtcaga tctcgttttc cttaaccatg ttacatagaa tgtgcacgga aatttaacct
     5521 gcctcatatt tggagcaaat atggaccgtg tccttcattt tgtactggca cttgccgttg
     5581 ttgctattct cgcactgctg gtaagcagcg accgcaaaaa aattcgtatc cgttatgtta
     5641 ttcaactgct tgttatcgaa gtgttactgg cgtggttctt cctgaactcc gacgttggtt
     5701 taggcttcgt gaaaggcttc tccgaaatgt tcgaaaaact gctcggattt gccaacgaag
     5761 ggactaactt cgtctttggt agcatgaatg atcaaggcct ggcattcttc ttcctgaaag
     5821 tgctgtgccc aatcgtcttt atctctgcac tgatcggtat tctccagcac attcgcgtgt
     5881 tgccggtgat tatccgcgca attggtttcc tgctctccaa agtcaacggt atgggcaaac
     5941 tggaatcctt taacgccgtc agctccctga ttctgggtca gtctgaaaac tttattgcct
     6001 ataaagatat cctcggcaaa atctcccgta atcgtatgta caccatggct gccacggcaa
     6061 tgtccaccgt gtcgatgtcc atcgttggtg catacatgac catgctggaa ccgaaatacg
     6121 tcgttgctgc gctggtactg aacatgttca gcacctttat cgtgctgtcg ctgatcaatc
     6181 cttaccgtgt tgatgccagt gaagaaaaca tccagatgtc caacctgcac gaaggtcaga
     6241 gcttcttcga aatgctgggt gaatacattc tggcaggttt caaagttgcc attatcgttg
     6301 ccgcgatgct gatcggcttt atcgccctga tcgccgcgct gaacgcactg tttgccaccg
     6361 ttactggctg gtttggctac agcatctcct tccagggcat cctgggctac atcttctatc
     6421 cgattgcatg ggtgatgggt gttccttcca gtgaagcact gcaagtgggc agtatcatgg
     6481 cgaccaaact ggtttccaac gagttcgttg cgatgatgga tctgcagaaa attgcttcca
     6541 cgctctctcc gcgtgctgaa ggcatcatct ctgtgttcct ggtttccttc gctaacttct
     6601 cttcaatcgg gattatcgca ggtgcagtta aaggcctgaa tgaagagcaa ggtaacgtgg
     6661 tttctcgctt cggtctgaaa ctggtttacg gctctaccct ggtgagtgtg ctgtctgcgt
     6721 caatcgcagc actggtgctg taaaaccata cataaaaaag ccggggatca ttccccggct
     6781 tttttacgcc tgttaatcag ctaatggctg cgggcgacca atcaaatacc cttgcagata
     6841 ttgcaccccg agcttatgca atagcgcctg ctgctgttgc gtctcgacaa actccgcgac
     6901 cacactcaat gacttcgctt ttgccagatc ggtaattgat ctcacaatca tcgcatccag
     6961 cgtattcgtg acaatatctt tcacaaagac gccatcaatt ttgatgatat cagcctgcaa
     7021 acgctttaac cgttcgtagt tggcatatcc ggtgccaaag tcatcaatcg caatccggaa
     7081 accaaactta tgcagctgct cgatgttgta catgctgctt tctgcgttag aaaacgcctg
     7141 ctcctcggtg atctcaagaa tgaccgcctg cggggagatg tgataacgct taaacagacg
     7201 aataatccgc ccggcaatat tcttttgcag cagcgtgagc ggcattaaat tgactgaaaa
     7261 gcgcggtcct tttttgtcgc aagggtgtgt tgccagccac ttcaacaagg attccagcac
     7321 ttgcaaatca aaacgcgcgc taaggttaaa ctgagcaata aggggcagaa acttatccgg
     7381 ggtcataatg ccgccgtcat atttcagtcg cgcgaggatc tcatcataac cttcaccctc
     7441 tttgttgcga attggctggg cgtagagcag caaatcaccc tgatctaacg ccgtgcgaat
     7501 ggtattcagc aatagcacct gtttagtggt ctgcccggaa accatctcct ctctgctatc
     7561 cagcgccagc acatgatgat gtgcgcagga ttgctccgcc agccagctta actgccccaa
     7621 caagggttgc agggtttcct gatttccatc aaaacgcccc caggcagcac catagcccat
     7681 atccagcccg gtattgttcc agtgaatttg ccgactattc aggatgttaa ccatatgctg
     7741 gagtcgccct tccgtttccg gcccgctcag cactaacagc agttcactac ccggcaattg
     7801 atacaacttt tcgttttcct gcatcaacgg cagcagcgta cggcaaattg agcggataca
     7861 gtgaacgcgc atcattaagc cgtaatgacg actcataaac tcaagattat caatgcgcag
     7921 gcagcaaaaa ctcttgcccg cctcttgctc cggcgcttgt tccaacgcac gaaagttggg
     7981 taggagcgtt aacggatctg tcagcgcctg caaatgccag cggcgattaa gccattcact
     8041 gcgatgataa atgcgcacca tatagagcag gcaaacgcta aaggaaatca gcaccgcgag
     8101 aataaatgcc agcgaatatt cggtttccac cccttgcaaa aagttctggt tgtaattcag
     8161 aaggcaaagc gttgaaacag cccaggtgag atttaaaaac ggatagcgaa gattaccgac
     8221 cccgagggta aagatgatga agaaaacggg taccaggtaa ccggcaataa agtcgttttc
     8281 ataaggtgtg cacaacagaa gtagcagcac gcttaacgct gccagccagc ttaaggtaaa
     8341 tgcgcgtttc tctttgccca acgacggagc gatatccctg cgccacaata tctgcgcaaa
     8401 gtggggactt acaatcatgc gagtgagata gtagaaaagc atgttgtaaa tcagcactgc
     8461 ggtgaaaagg cttagtaaat caacgaccgt gaaaatggca tccgcatcgc cgaaaaaggt
     8521 ggatatcttt agcgggaaat caaagaagct ccccacaaga tacatgctgc atttgatgcc
     8581 gatcggcgtc accaaaccaa gccagaacag acgttgccag acatatcggc tggtcaatcc
     8641 ataacgccaa cgcgtcccca gctgccagcg taatattgcg caggcacaca acacgacaaa
     8701 cgtctggctg ccgagcatga ccgcagtctg taaaaaagag agatgaaagt tccacagatt
     8761 ggtgcaaaac atcccgcata aaacgggaac gacaccgcgc cagccaaaga taaacaagat
     8821 tgacagcatt acgcacagtg gcatccacgc cagaaagata taactggaat ggataatggc
     8881 aagtggcgaa ataaaacggg atagctgaat aagtaccacg gtgagcgtaa acgctagtgt
     8941 gaatatcttg atatttttta tcaggttatg ctccacaaac atgagtacac tactctcact
     9001 gatcggggtt ttcaggcaca caaataataa gtttattcgc atcgcgaagc aaacaggggc
     9061 cagcctgttg ccgatttttc atccaatgat gctttcacca ggcggcgcta acccggacgt
     9121 agactgtatt acaaaagcgg caaaaagcag agacaaaaaa cccccgcttt gcagcgaggg
     9181 ttg
//
LOCUS       DADLSB010000149         9164 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.149, whole genome shotgun sequence.
ACCESSION   DADLSB010000149 DADLSB010000000
VERSION     DADLSB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.149"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            305..457
                     /locus_tag="JN331_003327"
     CDS             305..457
                     /locus_tag="JN331_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7261388.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(579..1199)
                     /gene="entD"
                     /locus_tag="JN331_003328"
     CDS             complement(579..1199)
                     /gene="entD"
                     /locus_tag="JN331_003328"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAY7261389.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1356..3596)
                     /gene="fepA"
                     /locus_tag="JN331_003329"
     CDS             complement(1356..3596)
                     /gene="fepA"
                     /locus_tag="JN331_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAY7261390.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADARDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDMMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3839..5041
                     /gene="fes"
                     /locus_tag="JN331_003330"
     CDS             3839..5041
                     /gene="fes"
                     /locus_tag="JN331_003330"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAY7261391.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5044..5262
                     /locus_tag="JN331_003331"
     CDS             5044..5262
                     /locus_tag="JN331_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAY7261392.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5259..9140
                     /gene="entF"
                     /locus_tag="JN331_003332"
     CDS             5259..9140
                     /gene="entF"
                     /locus_tag="JN331_003332"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAY7261393.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQWYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
BASE COUNT         1987 a         2423 c         2527 g         2227 t
ORIGIN      
        1 atggtaaggc acctgcttta cactttcgcc cgcggtcagt gatggctgcg ggcgatttcg
       61 ttttaagagc aatgatgagt tttgccgaca gaagtccgaa acaggactca ctgtataaat
      121 aaacagctat tttgctaagg aagggtaaga taacggcggg tgcctgaagc tttccggttt
      181 caggttaact ctgaggtttg gaaagatgaa gccccaagag atatttctat caatcctggg
      241 gctgccactc cgaacctcaa caactcggat ggtagtccct tcttcgcatg gaggcaatat
      301 aaacatgctg acgaaatatg cccttgtggc agtcatagtg ctgtgtttga cggtgctggg
      361 atttacgctt ctggtcggag actcgctgtg tgagttcacc gtaaaggaac gtaatattga
      421 gtttaaggct gttctcgctt acgaaccgaa gaagtagccg ttgtgcgggg agtaatcccc
      481 gcatatccgg ttgtcaggtc agagtggtaa ggcacctgct ttacactttc gcccgcagtc
      541 agtgatggct gcgggcgaat cgtaccagat gttgtcaatt aatcgtgttg gcacagagtt
      601 atgactattt tttcttttat ctgccagtgc acagcaaaca tctcattctc acgatgaatg
      661 atgacctgct gtttattcca gctaattatc tgatagtcca gaaaacctgc atcagtttgg
      721 atctcacttg ccttaaatgc gctctctttg gcggaaaatg ccagtgtcag cgccagagaa
      781 aaggctaaac cgcagtctgc gagtcgctcg tgttctgctg gtgtaataat gttgtctgtc
      841 aattcggtcg ctgtttgcgc agaaaaaatt tcttcgatat caacgccaat cggttgacgg
      901 gaaaccacgg ccaatgccgt agccccgcag tggctaatac tgccgtatac ctccgcaggc
      961 cacaccggtt ggcgtagctc gccgattgcg ggcacacatt tatagccata ttcccgcaag
     1021 gcataaacag cagcgatccg tccggctaaa tgctctgttt tacgtttacg tccagcgtgt
     1081 tgcagttgtg cgtagtgcgg cagccagagt aaatcctgct cacaaaaatt cgccggatcg
     1141 aactcaacaa aatgcagcgt atgtccggca aaggggaggg aggtatgcgt agttttcata
     1201 tcgaccatat tcgagactga tgacaaacgc aaaactgcct gatgcgctac gcttatcagg
     1261 cctacgtgat cctgcaatat attgaattgg caagatcttt gtaggccgca tccggcgtga
     1321 acgacgcgca ctttgtcaac aatctgacgt tagcatcaga agtgagtatt aatgctcata
     1381 taccacgtac gtcccggctc gttataggta tacgcgccgg caccggcgat atagttggct
     1441 cctgccagat cgcccgtggt ctgggcatta cctgcacgcc acaaacgttt gtcgaacagg
     1501 ttgtccacgc cgccggtcag actgacattc ttcgtcacat cccaggtcgc gctcagaccc
     1561 acaatgctgt aaggactgat ttctttggtt tccgggccaa ccgctggctg acctttatag
     1621 ttgtacttct tcggctgctg cttgccgtac caggtgaagg tcgtttgcat cgacaaatct
     1681 tcccgtgcct gccagctcag cgttgagttc aacgtgtact ccgggatgat cgacaaacgg
     1741 tcgcccgtgg ttttgttttc actcttcagc atataagtga tgttattggt ccacatcacc
     1801 gtttcgctaa ctggtacgtt taacgatcct tccagacctt caaccactgc tttcggcacg
     1861 ttatcccact gatagagatc agtgccgact gcgttttgcc ctacagccac atagcccgct
     1921 tcaatcttat tgcgataatc gttacggaac caggtgacgc ccgccagcca gccgtcgcgt
     1981 ttgaactcca gaccaatctc tttgttgatg ctggtttctg ctttcaggtc atcgttacct
     2041 tgcagatagc agccgcccgc gctggcatag cagccctgac ctttactgta gagaatgtag
     2101 ttcgggttag tctggtacag gctcggcgct ttataggcgc gggcaatgcc tattttcagc
     2161 gtgaagtcat cgcctaaacc ttgcgatatg ttcagcgccg ggctccagtt attgccgaca
     2221 atactgtgat gatcgaaacg cagccccggc gttacgatgg tgctgtcagt cagctccatg
     2281 ttgttttcgg caaacagcga gaaaatttct gcttttgaat acgggctacg gtcggtggca
     2341 ctcacgccat caatagcgcc accggtattc gttccggtca gtgcctgggt gttggaactt
     2401 aagtccttca tccgttgctg attccactcc gtacccagcg tcagcgtctg gttaacgagg
     2461 aaatcaatcg gcaggttaac ttcgctgtgc agcatcatgt catcgagatc gatatcgacg
     2521 aaatcctgtg tcgctttttc gttaaatttc ccttcggtac cgcccgccag accttccgga
     2581 atacgcgagt tacgggtgtg ttcatactgc acccagttgc tggtggtcac gccgttatcc
     2641 cagccaccgt tccaggtcag cgcgtagttc tggcgataca ggcggttggt ttcatcgcca
     2701 tatttcgagc gagtgtaagc gtcagagttg gtgttctggg tatcgcccgc atacaggtta
     2761 ccctggcggc tgtaacccgc ttccagttcc agcgattgca atggtgcgaa gtcccagcgc
     2821 accacgccgt taatatcttt attgatcacc ccttcgcgcc cggctggtaa cgtcgtggca
     2881 tacgttccgg cacgcgcgga ctgatggccc tggttgatat ccctcgcgtc agcctgagtt
     2941 ttgtcgaggt tgccatacaa gcggaagctg aattcgtcgc ccagcggacc ggtcaggcta
     3001 aagttagtgc gtttggtggc accttcctct ttatgttctg gcgcattgaa atatgcgtcc
     3061 caggagccgt gccactcgcc gctgcctttt ttggtaatga tgttaaccac gccgcccgcc
     3121 gcgccgttgc cataacgcgc agctgccgga ccacgcagaa cttcaatacg ttcaatcatt
     3181 tcaggtggca cccaggaggt atcaccacgg gtatcgcgct cgccacgcca gccctgacgc
     3241 accgagttac ggctgcttac cggcttgccg tcaatcaaaa tcagcgtgtt ttccggaccc
     3301 ataccgcgaa tatcaatctg tcggttattc ccacgctgac cactggtgga gttaccggtc
     3361 aggttaacgc ctggcatggt acggatgatc tccgacacat cgcgggcaac cgggtttttg
     3421 cggatttcat ctgcggtgat ggtcgaaacg ccaggtgcct gcaagttctg ctcggcggcg
     3481 gtaacgacaa tggtatcgtc atgtgaaaca ggagtatcgg tcggctcttg tgcctgcgct
     3541 accccataaa tccccagatt gaccaacaag gccagggaat gaatcttctt gttcattgtt
     3601 ttattcctgc atttttgcca cgaattgcaa ctgtcgggca tggtcgtcat caacacgacg
     3661 catcccgtta ccgcgaaaac ctttgatcct gaaagacacg cagtgcagtt ggttaattaa
     3721 tgtccgcgct tcccacggcg cgccattacg ctattgtaaa tgcaaatagt tatcaataat
     3781 attatcaata tatttctgca atcaatgaaa aattgcacag taaacatggg gttatggtgt
     3841 gacggcgtta aaagtaggaa gtgagagctg gtggcagtcg aaacatggcc cggaatggca
     3901 gcgtctgaat gacgaaatgt ttgaggtcac tttctggtgg cgtgatcccc aaggttctga
     3961 agaatactcg acgataaagc gcgtatgggt ctacatcact ggtgtgaccg atcatcatca
     4021 gaacagccag ccccagtcga tgcagcgaat tgcaggtact gatgtctggc agtggacgac
     4081 acaactcaat gccaactggc gcggcagcta ctgctttatt cccaccgaac gcgatgacat
     4141 tttttctgca ccatcccccg atcgcctcga attacgtgaa ggctggcgaa aactattacc
     4201 ccaggcaata gccgatccgc tgaatccaca aagctggaaa ggcgggcgag ggcacgctgt
     4261 ttctgcactc gaaatgccgc aagcgcctct gcaaccggga tgggattgtc cgcaagcgcc
     4321 agaaatacca gccaaagaaa ttatctggaa aagtgaacgg ttgaaaaatt cacgtcgtgt
     4381 atggattttt accaccggcg atgtaacagc agaagaacgc ccgctggcag ttttgctcga
     4441 tggcgaattt tgggcgcaaa gtatgcccgt ctggcctgtg ctgacttcgc tgacccatcg
     4501 tcagcaactt cctcccgccg tgtatgtgtt gatcgacgct atcgacacca cgcaccgcgc
     4561 ccacgaactg ccgtgtaatg cggatttctg gctggcagta cagcaagagt tattacccct
     4621 ggtgaaagtt attgcccctt ttagcgatcg cgccgatcgc accgtggtcg ccgggcagag
     4681 ttttggtggg ctgtccgcgc tgtatgccgg actgcactgg cctgaacgct ttggctgtgt
     4741 attaagccag tcaggatcgt actggtggcc gcatcggggc gggcagcaag agggcgtgtt
     4801 acttgaaaag ctaaaagctg gtgaagttag cgccgaaggt ctgcgcattg tgctggaagc
     4861 gggtattcgc gagccgatga tcatgcgggc caatcaggcg ctgtatgcgc aattacaccc
     4921 tataaaagaa tccattttct ggcgtcaggt tgacggcgga catgatgcgc tttgttggcg
     4981 cggtggcttg atgcaggggt taatcgacct ctggcaacca cttttccatg acaggagttg
     5041 aatatggcat tcagtaatcc cttcgatgat ccacagggag cgttttacat tttgcgcaat
     5101 gcgcaggggc aattcagtct gtggccgcaa caatgcgcct taccggcagg ctgggacatt
     5161 gtgtgtcagc cgcagtcaca agagtcctgc cagcagtggc ttgaagccca ctggcgtact
     5221 ctgacaccgg cgaatttcac ccagttgcag gaggcacaat gagccagcat ttacctttgg
     5281 tcgccgcaca acccggcatc tggatggcag aaaaactgtc ggatttaccc tccgcctgga
     5341 gcgtggcgca ttacgttgag ttaaccggag aggttgatgc gccattactg gcccgcgcgg
     5401 tggttgccgg attagcgcaa gcagatactt tgcggatgcg ttttacggaa gataacggcg
     5461 aagtctggca atgggtcgat gatgcgctga tattcgaact gccagaaatt atcgacctgc
     5521 gaaccaatat tgatccgcac ggtactgcgc aggcattaat gctggcggat ttgcaacaag
     5581 atttgcgcgt cgatagcggt aaaccactgg tctttcatca actgatacag gtggcagata
     5641 accgctggta ctggtatcag cgttatcacc atttactggt cgatggcttc agttttccgg
     5701 ccattacccg ccagatcgcc aacatttact gcgcattgct gcgtggcgaa ccaacgcctg
     5761 cttcgccgtt tacgcctttc gctgatgtag tggaagagta ccagcaatac cgcgaaagcg
     5821 aagcctggca gcgtgatgcg gcattctggg tggaacagcg tcgccaactg ccaccgcccg
     5881 cgtcactttc tccggcacct ttagcggggc gcagcgcctc ggcagatatt ttgcgtctga
     5941 aaatggaatt taccgacggg gaattccgcc agctggctac gcaactttca ggtgtgcagc
     6001 gtaccgattt agcccttgcg ctgacggctt tgtggctggg gcgattgtgc aatcgcatgg
     6061 actacgccgc cggatttatc tttatgcgtc gactgggctc ggcggcgctg acggctaccg
     6121 gacccgtgct caacgttttg ccgctgggta ttcatattgc ggcgcaagaa acgctgccgg
     6181 aactggcaac ccgactggca gcacaactga aaaaaatgcg tcgtcatcaa cgttacgatg
     6241 ccgaacaaat tgtccgtgac agcgggcgag cggcaggtga tgaaccgctg tttggtccgg
     6301 tactcaatat taaggtattt gattaccaac tggatattcc tggtgttcag gcgcaaaccc
     6361 ataccctggc aaccggtccg gttaatgacc ttgaactggc cctgttcccg gatgaacacg
     6421 gtgatttgag tattgagatc ctcgccaata aacagcgtta cgatgagcca acgttaatcc
     6481 agcatgctga acgcctgaaa atgctgattg cccagttcgc cgcggatccg gcgctgttgt
     6541 gcggcgatgt cgatattatg ctgccaggtg agtatgcgca gctggcgcag atcaacgcca
     6601 ctcaggttga gattccagaa accacgctga gcgcgctggt ggcagaacaa gcggcaaaaa
     6661 caccggatgc tccggcgctg gcagatgcgc gttacctgtt cagctatcgg gaaatgcgcg
     6721 agcaggtggt ggcgctggcg aatctgctgc gtgagcgcgg cgttaaacca ggggacagcg
     6781 tggcggtggc actaccgcgc tcggtctttt tgaccctggc actccatgca atagttgaag
     6841 ctggcgcggc ctggctaccg ctggataccg gctatccgga cgatcgcctg aaaatgatgc
     6901 tggaagatgc gcgtccgtcg ctgttaatta ccaccgacga tcaactgccg cgctttagcg
     6961 atgttcccaa tttaaccaac ctttgctata acgccccgct taccccccag ggcagtgcgc
     7021 cgctgcaact ttcacaaccg caccacacgg cttatatcat ctttacctct ggctccaccg
     7081 gcaggccgaa aggggtaatg gtcgggcaga cggctatcgt caaccgcctg ttgtggatgc
     7141 aaaatcatta tccgctcaca ggcgaagatg tcgttgccca aaaaacgccg tgcagttttg
     7201 atgtctcggt gtgggagttt ttctggccgt ttattgccgg ggctaaactg gtgatggctg
     7261 aaccggaagc gcaccgcgac ccgctcgcta tgcagcaatt ctttgccgaa tatggcgtaa
     7321 cgaccacgca ctttgtgccg tcgatgctgg cagcatttgt tgcctcgctg actccgcaaa
     7381 ccgctcgcca gagttgcgtg acgttgaaac aggttttctg tagtggtgag gccttaccgg
     7441 ctgatttatg ccgcgaatgg caacagttaa ccggcgcgcc gttgcataac ctgtatggcc
     7501 cgacggaagc ggcggtagat gtcagctggt atccggcttt tggcgaggaa ctggcacagg
     7561 tgcgcggcag cagtgtgccg attggttatc cggtatggaa tacgggtctg cgtattcttg
     7621 atgcgatgat gcatccggtg ccgccgggtg tggcgggtga tctctatctc accggtattc
     7681 aactggcgca gtggtatctt ggacgacccg atctgaccgc cagccgcttt attgccgatc
     7741 cttttgctcc tggtgaacgg atgtatcgta ccggagacgt tgcccgctgg ctggataacg
     7801 gcgcggtgga gtacctcggg cgcagtgacg atcagctaaa aattcgcggg cagcgtatcg
     7861 aactgggcga aatcgatcgc gtgatgcagg cgctgccgga tgtcaaacaa gccgttaccc
     7921 acgcctgtgt gattaaccag gcggcagcca ctggtggtga tgcgcgtcag ttggtgggct
     7981 atctggtatc gcaatcgggc ctgccgttgg ataccagcgc attgcaggcg cagcttcgcg
     8041 aaacattgcc gccgcatatg gtgccagtcg ttctgctgca actgccgcag ttgccactta
     8101 gcgccaacgg caagctggat cgcaaagcct taccgttgcc tgaactgaag gcacaagcgc
     8161 cagggcgtgc gccgaaagcg ggcagtgaaa cgattatcgc cgcggcattc gcgtcgttgc
     8221 tgggttgtga cgtgcaggat gccgatgctg atttctttgc gctgggcggt cattcgctac
     8281 tggcaatgaa actggcagcg cagttaagtc gacagtttgc tcgtcaggtg acgccggggc
     8341 aggtgatggt cgcgtcaacc gtcgccaaac tggcaacgat tattgatggt gaagaggaca
     8401 gctcccggcg catgggattc gaaaccattc tgccgttgcg tgaaggtaat ggcccgacgc
     8461 tgttttgttt ccatccggcg tccggttttg cctggcagtt tagcgtgctc tcgcgttatc
     8521 tcgatccaca atggtcgatt attggcattc agtcgccgcg ccctcatgga cccatgcaga
     8581 cagcgacaaa cctggatgaa gtctgcgaag cgcatctggc aacgctactt gagcaacaac
     8641 cgcacggccc ttattacctg ctgggttatt cacttggcgg tacgctggcg cagggcattg
     8701 cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg gatacctggc
     8761 cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg gaagtgctgg
     8821 cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg
     8881 agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc
     8941 atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag
     9001 gtatgagccc cgaacgcgct tggtcgccgt ggattgcgga gctggatatc tatcgtcagg
     9061 attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg ccgattattc
     9121 gcgcaacgct aaacaggtaa attaatatta tctataaacc cata
//
LOCUS       DADLSB010000150         9132 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.150, whole genome shotgun sequence.
ACCESSION   DADLSB010000150 DADLSB010000000
VERSION     DADLSB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.150"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(310..1014)
                     /locus_tag="JN331_003333"
     CDS             complement(310..1014)
                     /locus_tag="JN331_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7261394.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1151..1603)
                     /gene="moaE"
                     /locus_tag="JN331_003334"
     CDS             complement(1151..1603)
                     /gene="moaE"
                     /locus_tag="JN331_003334"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY7261395.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1605..1850)
                     /gene="moaD"
                     /locus_tag="JN331_003335"
     CDS             complement(1605..1850)
                     /gene="moaD"
                     /locus_tag="JN331_003335"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY7261396.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1843..2328)
                     /gene="moaC"
                     /locus_tag="JN331_003336"
     CDS             complement(1843..2328)
                     /gene="moaC"
                     /locus_tag="JN331_003336"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY7261397.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2331..2843)
                     /gene="moaB"
                     /locus_tag="JN331_003337"
     CDS             complement(2331..2843)
                     /gene="moaB"
                     /locus_tag="JN331_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY7261398.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREFEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2865..3854)
                     /gene="moaA"
                     /locus_tag="JN331_003338"
     CDS             complement(2865..3854)
                     /gene="moaA"
                     /locus_tag="JN331_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY7261399.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDSIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3842..3986)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4251..5159
                     /locus_tag="JN331_003339"
     CDS             4251..5159
                     /locus_tag="JN331_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAY7261400.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASTMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5351..7372)
                     /gene="uvrB"
                     /locus_tag="JN331_003340"
     CDS             complement(5351..7372)
                     /gene="uvrB"
                     /locus_tag="JN331_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY7261401.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7951..8628)
                     /gene="bioD"
                     /locus_tag="JN331_003341"
     CDS             complement(7951..8628)
                     /gene="bioD"
                     /locus_tag="JN331_003341"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7261402.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTV
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGELPWLAENPENAATGK
                     YINLALL"
     gene            complement(8621..>9132)
                     /gene="bioC"
                     /locus_tag="JN331_003342"
     CDS             complement(8621..>9132)
                     /gene="bioC"
                     /locus_tag="JN331_003342"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY7261403.1"
                     /translation="KDAADHYLAGDIESLPLATATFDLAWSNLAVQWCGNLSTALREL
                     YRVVRPGGVVAFTTLVQGSLPELHQARQAVDERPHANRFLPPDEIEESLNGVRYQHHI
                     QLITLWFDDALSAMRSLKGIGATHLHEGRDPRILTRSQLQRLQLAWPQQQGRYPLTYH
                     LFLGVIARE"
BASE COUNT         2194 a         2433 c         2271 g         2234 t
ORIGIN      
        1 tgcggttgcc accaaaccta aagcggtaat gagccatata ccgtttaata tcctggcctc
       61 gtgctgaaaa tctaatttat tgctgttttg cgagtatgat tccattcttt tagttgtcct
      121 tagaaaaaat attaatattc accatcaata aatttaaaac cacgcataca aaatactttt
      181 gtatttatgg atacggaaaa cgattccgct cgctcataag gcatatttta tacctggtac
      241 ttgttaagtg tcgtttttag taagaaaaaa atcccgacac atggccggga tagtgatggt
      301 gaaaaagaat taacggcggt tgccgaagat ccgtaacaac atcaggaaca ggttgatgaa
      361 gtccagatac aaggttaacg cgccaagaat ggaatatttg cgcaggttcg acgtgtcgcg
      421 ggtatcaatc tgctcaccca tatttttcaa tttctgagtg tcatacgccg tcaatccgac
      481 aaagacaatc acgccgatgt aggtaactgc ccacatcaat gcttcgcttt tcagccagaa
      541 gttgaccagc gatgccagca caatgccgat taacgccata aacagcatat tgccaaagcc
      601 acttaaatcg cgcttcgtgg tgtaaccgta caggctcatt gcgccgaaca tcccggcagt
      661 aacgacgaaa gtactggcga tagaagcagc ggtatagaca atgaatatac tggaaagcgt
      721 aagacccgtc agcgccgaat aaagcataaa gagcatcgtc gttacacctg cgctcagctt
      781 ttgaatcatc gctgataaca caataaccaa tgctaattgc gcgatgatca gaccgattaa
      841 aaagacacgg ttagtgaaca acagctccat cacggccgcg gaattagccg cataccaggc
      901 aacaaatgcg gtcagcaaca agccaacggt catccagcca tagacttgcg ccatataagt
      961 ttgcaagcca gcccggggtt gtacgattga atcagaacgt gggaatctgt ccatgacgat
     1021 ctcctgaaga tataaggaat atcttaagga tactgcaaaa tgatgaggct gtgcatcgac
     1081 gcagcgtaaa cgcatgtact gagcggtgaa attgccggac gcagcggtgc cttatccggc
     1141 taacaaaaaa ctaccagcgt tttgccgcct gcagatcgct ctcccgggct tcaacccagc
     1201 ggtcgccttc cggcgtggct tcgcgcttcc agaacggtgc gcgggttttg agataatcca
     1261 taataaactg cccggcttca aacgcactgc tgcgatgcgc actggtgaca ccgacaaaaa
     1321 cgatttcatc gcccggccat aattccccga tgcggtgaat caccgtgacg cgccccagcg
     1381 gccagcggtt acgcgcttca tcaacaattt ctgccagtgc tttttcagtc atccccggat
     1441 agtgttcgag ggttaatgct ttgacgctgt cgcccaggtt atggttgcgc acctttccgg
     1501 tgaaggtgac taccgcaccg tcttcgtcac gctccgccag ccacgggtac tcttctccta
     1561 cgctgaacgg ctgcgggccg acaacaattt tggtttctgc catcttaacc tccggttacc
     1621 ggcgggaaga aagctacttc gtcgccgtca gtcagcggat ggtcaaaact caccagcgtc
     1681 tggttgacgg cagccagcaa tttgccatct tccagcgcca gcgcccagcg atcgctctgc
     1741 gcagccaggt gctggcgtaa cgcttcaacg gttgggtaat ccgcagccac ttcggttgca
     1801 tctgttccca ccaactcgcg cacctgggcg aaaaaaagaa ctttaatcat ccgcttccac
     1861 cttaaagtca cccgacttgc cgccgctctt cgccagcaaa cgtaccggac caatcaccat
     1921 atctttttgc accgctttgc acatgtcata aatggtcagc gccgccacgg aggccgcggt
     1981 taacgcttcc atttcgacac cggttttccc ggtcaggcgg cataaggttt ctatacgcac
     2041 ccgattgtgc tccggctcgg cctgtaaatt gacttcaact ttgctgagca tcagcgggtg
     2101 acacagcggg atcagatccc aagtgcgttt tgccgcctga ataccggcaa tacgcgcagt
     2161 ggcaaatacg tcgcctttgt ggtggcgacc atcaataatc atcgccagcg tctcgctgcg
     2221 catggtgaca aaggcttccg cccgcgcttc acgcacggtt tccgctttgg cggagacatc
     2281 caccatgtgc gcttcgccag cggcgttgat atgggtcagt tgcgacatac ttatttcttc
     2341 aaatgtggat ggaaattaca cggacgcgta cgggcatcca gctgcggcgc gatgatattt
     2401 tcccatgcgg tacggcacgc tttggtcgaa cccggcatgg cgaaaatcag cgttttgtta
     2461 gcgacgcccg ctaccgcacg agattgcaac gtggaagtgc caatctcttc aaacgacaac
     2521 atacggaaca cttcaccaaa accttcaaac tcacggtcga acaacggcag cagcgcttcg
     2581 ggagcctgat caccttccgt caggccagta ccacccgtaa tcaataccac ttgtacatcg
     2641 tcgctggcga tccacgcaga tacctgagcg cgaatagcgt agcggttttc tttcacaatg
     2701 gctttatcga caacgtgatg gcccgcttct tgcgccgaat cgcgcagata gtgaccggag
     2761 gtatcgtctt cttcaccgcg acgattagaa accgtaagaa tagcaatacg ggtcgggata
     2821 aattcagtgc ttacctgact catctgatct ctccttttga cgttttagcc gccaatgtac
     2881 gataagtttt gcgtaatacc ggtgttgttt tgatgcagga aatgggtctg tttcttctcc
     2941 cgcagcgccg ctgaaatacg cgcttccagc gcctgttgct gggtatcgtc ttccagcaga
     3001 tcgcgcaggt taacgccgcc ttcaccaaac aggcagagat ggagtttacc aatggaggaa
     3061 acgcgcaggc ggttgcaagt ggcgcagaag tctttttcat acggcatgat aaggccaatc
     3121 tctccggcgt aatctggatg gcaaaagact tgcgcgggac cgtcgctgcg ttgacgtaat
     3181 tggtggatcc agccgcgacg cagtagctcg tcacgcagaa cctgaccaga gatgtgatgc
     3241 ttacggaaga gctcgctgcc ctcgcccgtt tccatcagtt cgatgaaacg cagctggata
     3301 gggcgatgct ggatccagtt cagaaaggtg tcgagctggt gatgattaac atcacgcatc
     3361 agcacggtat tgactttgac cttctcaaaa ccggcctcaa atgcagcatc aatccctgcc
     3421 atgacctggt tgaatttatc ctgcccggta atagcgtgaa actggcgggc gtccaaactg
     3481 tcgacactga cgttaatgcc agtaagtccc gcatcgcgcc agttcgccac atcgcgttcc
     3541 agacggtaac cattggtggt gaccgcaatc tggcggatag agtcgttttc ccgcacagcg
     3601 gcgatgatat cggtaaagtc gcggcgtaaa gacggctctc ctcctgtcag gcgcactttt
     3661 tcggtgccca gactggcgaa ggcgcgcgta acccggcgaa tttcatcgac ggtaagaaag
     3721 cctttattgg tgacgccgct cggtttgtag ccatccggca ggcagtaggt gcaacgaaag
     3781 ttacacacat cggtaatcga caggcgcaag tagtaaaact tacgcgcaaa tgcatcagtc
     3841 agttgtgaag ccatgtacac ctttccagat acgggaggcg aagtcatttc ttccttcgcc
     3901 ctggtggcaa tctttacgag cattgtcacg gccaaagcac cgtatcagtt gacccaggtg
     3961 cagaggctag agtgtttagt ggttaagccg atactagcgt gtaaatgaga tttttaccat
     4021 ccacactttc gctatgtaat catgtatata gcgtcatgat cgtgctattt cgctacagaa
     4081 taagggaaaa tggcggtttt actttgatat acatcatcag ggaggcatag cgtgcatcgt
     4141 cctttagaga tagaagaaaa tagtttgtat cctcaatatt ggcaggttaa ttgctgtttc
     4201 cccgcaattt gcgctactgt agcgcggata atctgactcc aggaaactat atgcgcaatc
     4261 gtacgctggc tgatcttgat cgtgtcgttg ctctcggcgg agggcatgga ctgggacgcg
     4321 ttctctcatc actttcgtct ttgggttctc gtttaacggg tatcgtcacc accacggata
     4381 atggtggctc gacggggcgt attcgccgtt cagaaggcgg cattgcttgg ggcgatatgc
     4441 gcaactgcct caaccagctg ataacggaac cgagcgtcgc ctccacgatg tttgaatacc
     4501 gttttggcgg caatggcgaa ctttccggtc ataatctcgg aaacttgatg ttaaaggcgc
     4561 tggatcacct tagcgtgcgg cctctggaag ccatcaattt aattcgtaat ctgctgaaag
     4621 tggatacgca tttgattcca atgtcagagc atcctgttga tctgatggcg attgacgatc
     4681 aggggcatga agtttacggc gaggtcaata tcgaccagtt aactacgccg attcaagagt
     4741 tattgttaac gcctaatgta cccgcaacgc gtgaggcggt tcacgctatc aatgaagcgg
     4801 atctcatcat tattgggcct ggcagttttt ataccagcct gatgccaatt ctgctgctga
     4861 aggaaatcgc ccaggcatta cgccgcacgc cagcgccgat ggtttatatc ggcaatctgg
     4921 ggcgtgagtt gagtttacct gcggctaatt tgaagctgga aagcaagctg gcaattatgg
     4981 agcagtatgt tggtaaaaag gtcattgatg cggtcatcgt cgggccaaaa gtggatgtct
     5041 cggcggtgaa agagcggatt gtgatccagg aggtactgga ggccagcgat attccgtatc
     5101 gtcatgaccg ccagttgtta cataacgcgc tggaaaaggc gttacaggct ttaggttaac
     5161 ggaaatccga aggaaaattc cggcttccta ttgaagacaa agtgcgcgtt gtttatgccg
     5221 gatgcggcgt gaacgcctta tccggcctac aaaccgcgca aattcaatat attgcggaga
     5281 aaatgtaggc ctgataagcg tagcgcatca ggctgttttc cgtttgtcat cagtcttctt
     5341 cgctatcctg ttacgatgcg gcaataaaca actcacgcaa ctgatgcaac tggtcacgaa
     5401 tttgtgccgc ttcttcgaac tccagattct gcgcgtgttg catcatcagc ccttccagtt
     5461 catggatttt ctgctgcaac gctttcggcg acatatccat cggcacatta tccggctcaa
     5521 caatcgggcg cgattttcct ctgcccttcg ctttggtttt ggcaatgttc tgccccagcg
     5581 ccaggatatc gaccactttc ttgttcaagc cttgcggcgt aatgccgtgt tcctcgttgt
     5641 acttctgctg tttctcacgg cgacgttcgg tttcgccaat cgctttcgcc attgatggag
     5701 tgatcttatc gccgtagaga atcgctttac cgttaacgtt acgtgccgca cgaccaatgg
     5761 tctggatcaa cgaacgttcg gaacgcagga agccttcttt gtcagcgtcg aggatcgcca
     5821 ccagcgacac ttccggcata tccagacctt cacgcagtaa gttgatccct accaatacgt
     5881 cgaactcacc cagacgcaag tcgcggataa tctccatacg ctcgacggtg tcgatatctg
     5941 agtgaagata acgcacgcgc tcaccgtgtt cttcgagata ttcggtaaga tcttccgcca
     6001 ttcgcttggt cagtgtggtg accagcacgc gttcgttaat tgccgctcgc tgacgaatct
     6061 ccgaaagaag atcatcaacc tgtgtcgcca ccggccgcac ttcgataatc gggtcaagca
     6121 atccggttgg acgcaccacc tgatccacca catcgccgcc ggatttttcc agctcgtaat
     6181 tacccggcgt cgccgaaaca tagatggttt gcggcgctaa tgcttcgaac tcttcaaact
     6241 taagcggacg gttatccagc gctgatggca ggcggaagcc gtactccacc agtgtctctt
     6301 tacgcgcccg gtcaccgcga tacatgccgc caatttgtgg aatggtgacg tgagattcat
     6361 cgacgaccag cagcccatcg gcaggcaggt aatcaaacag cgtcggcggt ggctcacccg
     6421 gtccacgacc ggagaggaag cgcgagtagt tttcaatccc cgaacagtag cccagctcgt
     6481 tcatcatctc cagatcaaac tgggtacgct gggtcagccg ctgctcttcc agcagtttgt
     6541 tgttttccaa cagcactttg cgtctggcgg ccagttcttc tttgatctcc tccatcgcct
     6601 gtacgatgcg ctcgcgcggt gtgacgtagt gcgttttcgg gtagatggta aaacgtggaa
     6661 tagtggaaac aatctgcccg gtcagcgggt caaataacga caatcgttcc acttcctcgt
     6721 caaacagttc cacgcgaagt gcaatgtcat ccgattctgc cgggaagata tctatcacct
     6781 cgccacgaac gcggaaagta ccacgctgga atgcttgatc attacgagcg tattgcagct
     6841 ccgccagccg gcgcagaatc gcgcgctgat cgataatcat accgaccgtg agatggagca
     6901 tcatcttgag atataaatca ggatcgccca gaccataaat cgcggaaaca gacgccacta
     6961 caatcacatc acgccgctcc agcatcgctt tggtggcgga caaacgcatc tgctcaatat
     7021 gttcgttaac cgaggcatct ttctcaatga aggtgtcgga actcggtaca taggcttccg
     7081 gctgatagta gtcgtagtag gagacgaaat actccaccgc gttttccggg aagaactctt
     7141 tcatttcgcc atacagctgg gccgccagcg ttttgttggg cgcaagtacc atggttgggc
     7201 gctgaaggtc agcaatgaca ttggcaatgg tgaaggtttt cccggagcca gtcacgccaa
     7261 gtaacgtctg gtgcgccagg ccatcttcca gcccctcttc gagacgtcga atcgcctctg
     7321 gctgatcgcc agaaggttta aaagcggaat tcagtttgaa cggtttactc atgagtcgct
     7381 acctgaagga gttgggcggg caggtatgta attttactcg tcgtacttaa ttatgccaac
     7441 aaattatact ggataaaaaa acagttcatc accataatat ttctgataca gcgtaaactc
     7501 cgctgtcaat catgagcaaa atttactctg tggcgagata aaactccggc cttaccgggt
     7561 tatccccaaa gcaacggctt ttttaacatt tgtcaagatg agtgatgaca gttttatggc
     7621 aagagatgcc tgttcagtga ctgccattgc atttatctaa ccagttaaaa aataaaagat
     7681 atttctttga gccgtcttta acgccaattc gcgtgcaagc cgcgtattct ctcgcttgcc
     7741 tcgtgttttc taactctaat acacatggtt atccacagga atagtggata actgtctcca
     7801 gcccctatcc cccgccgcct gggcaattcc cacattcgcc gcatgaggcg gaagtaaaat
     7861 ttttttcgtg attattttag aatttaattg gttaaattgc agtcaatcga agacgcgatc
     7921 tcgctcgcaa tttaaccaaa tacagaatgg ctacaacaag gcaaggttta tgtactttcc
     7981 ggttgccgca ttttctggat tttctgcaag ccaggggagc tctcccagca gcggcgcggg
     8041 aatcatgcgg gtgagcgtgg tcatatattc agcgtgacgt tttcccggag gcgtaacatc
     8101 gttcgccacc caacccgcca gagtcagtcc ggcgtgttgt attgcctgca cagtcaacat
     8161 cgcgtgatta atacagccga gtttcacacc aactaccagt atcaccggca gttgttcctg
     8221 tgttacccaa tctgcaaaag tgaaagtgtc agaaagcggc gtaaaccagc cgccagcacc
     8281 ttccactaac acccagtcag cctgttgttc aagcgcgcgt aatccggcgc tcattaccaa
     8341 tgattctatc ggcctgccct cttgggcgct gatgatgtgc ggcgaggtgg gttctgcgaa
     8401 ggtgtaagga tttaccgttg cgtaatccag ctgcaggctg ctgttgtgct gtaacgccag
     8461 cgcgtcacta ttacgtaagc cttccggggt cttttcgctg ccagaggcga ccggtttata
     8521 acctgccgtc cgataaccta ctgccttcgc ggcttgtaaa agtgcacaac tggcgacggt
     8581 tttccccact tcggtatccg ttccggtgac aaaataacgt ttactcacga gcaatcactc
     8641 ccaaaaaaag atgatacgtc agaggatatc gcccctgttg ttgcggccag gccagttgca
     8701 atcgctgcaa ctgcgaacgc gttaatattc gcgggtcgcg cccttcatga agatgcgtag
     8761 caccgatgcc tttcagcgaa cgcatggcac tgagcgcatc atcaaaccat agtgtgatga
     8821 gctgaatatg atgttgataa cgcacgccgt tcagcgactc ttcgatttca tctggcggta
     8881 aaaagcgatt agcatgcgga cgctcgtcca ccgcctgccg cgcctgatgc agttcgggta
     8941 acgatccctg caccagcgtg gtaaacgcga ccacgcctcc ggggcgcacc acccgataca
     9001 gctcgcggag tgccgtggat aaattaccgc accactgcac cgcgagattg ctccatgcaa
     9061 gatcgaacgt cgcagtcgct aacggcaggg attcgatatc tcccgccaga taatgatctg
     9121 cggcatcttt at
//
LOCUS       DADLSB010000151         9104 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.151, whole genome shotgun sequence.
ACCESSION   DADLSB010000151 DADLSB010000000
VERSION     DADLSB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.151"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            45..1103
                     /locus_tag="JN331_003343"
     CDS             45..1103
                     /locus_tag="JN331_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HAY7261404.1"
                     /translation="MEEDFRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVG
                     GSYHLAESYPPVHYIATDNYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYA
                     FRQLVAEEKYRGVVYQGLETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQ
                     VCEHLHIPVPEKLCVIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(1211..2035)
                     /locus_tag="JN331_003344"
     CDS             complement(1211..2035)
                     /locus_tag="JN331_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY7261405.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            2355..4385
                     /gene="malS"
                     /locus_tag="JN331_003345"
     CDS             2355..4385
                     /gene="malS"
                     /locus_tag="JN331_003345"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7261406.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            4563..5816
                     /gene="avtA"
                     /locus_tag="JN331_003346"
     CDS             4563..5816
                     /gene="avtA"
                     /locus_tag="JN331_003346"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY7261407.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(5967..6440)
                     /locus_tag="JN331_003347"
     CDS             complement(5967..6440)
                     /locus_tag="JN331_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7261408.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(6542..7390)
                     /gene="yiaJ"
                     /locus_tag="JN331_003348"
     CDS             complement(6542..7390)
                     /gene="yiaJ"
                     /locus_tag="JN331_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY7261409.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            7591..8589
                     /gene="yiaK"
                     /locus_tag="JN331_003349"
     CDS             7591..8589
                     /gene="yiaK"
                     /locus_tag="JN331_003349"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY7261410.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            8601..9068
                     /locus_tag="JN331_003350"
     CDS             8601..9068
                     /locus_tag="JN331_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7261411.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGQEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT         2303 a         2319 c         2266 g         2216 t
ORIGIN      
        1 taggggaata tttacaggca tcacaatcag aatgggatat tttcattgaa gaagatttcc
       61 gcgcccgcat tgataaaatc aaggactggt taggagatgt cgtcattgcc gacttcgacg
      121 acaaacagat cgagcaagcg ctggctgatg tcgacgtccc cattgttggg gttggcggct
      181 cgtatcacct tgcagaaagt tacccacccg ttcattacat tgccaccgat aactatgcgc
      241 tggttgaaag cgcatttttg catttaaaag agaaaggcgt taaccgcttt gctttttatg
      301 gtcttccgga atcaagcggc aaacgttggg ccactgaacg cgaatatgca tttcgtcagc
      361 ttgtcgccga agaaaagtat cgcggagtgg tttatcaggg gttagaaacc gcgccagaga
      421 actggcaaca cgcgcaaaat cggctggcag actggctaca aacgctgcca ccgcaaaccg
      481 ggattattgc cgttactgac gcccgagcgc ggcatattct gcaagtatgt gaacatctac
      541 atattcccgt accggaaaaa ttatgcgtga ttggcatcga taacgaagaa ctgacccgct
      601 atctgtcgcg tgtcgccctt tcttcggtcg ctcagggcgc gcggcaaatg ggctatcagg
      661 cggcaaaact gttgcatcga ttattagata aagaagaaat gccgctacag cgaattttgg
      721 tcccaccagt tcgcgtcatt gaacggcgct caacagatta tcgctctctg accgatcccg
      781 ccgttattca ggccatgcat tacattcgta atcacgcctg taaagggatt aaagtggatc
      841 aggtactgga tgcggtcggg atctcgcgct ccaatcttga gaagcgtttt aaagaagagg
      901 tgggtgaaac catccatgca atgattcatg ccgagaagct ggagaaagcg cgcagtctgc
      961 tgatttcaac caccttgtcg atcaatgaga tatcgcaaat gtgcggttat ccatcgctgc
     1021 aatatttcta ctctgttttt aaaaaagcat atgacacaac gccaaaagag tatcgcgatg
     1081 taaatagcga ggtcatgttg tagcgatgat gagaattgtc ggcgtcacat cagggaactc
     1141 agcacagcca cctgatgcga gcaaaagcct tatccgaccg acaaatcaga taaggctcga
     1201 ggaaatgaaa ttacatatgc gccgcgatta accgttggtt atcttggtac attgcgaaca
     1261 ggtagttgtt ataactcttc cctttggtcg agtagccctt cagcttgtga atcatcgctg
     1321 tggcagtcac ttcctgatcc gctttacgca gctgtgcacg cgatttacgg aacgaagagt
     1381 aagccgggtg cgtattcagg ttagtgacat aggcgctcac cgattctttg acagaactaa
     1441 actgtgagta ccctttcact ttacccggcg cattggtaca acgtcctttc atgcatttca
     1501 tgccgaacag attgttattg ttgcgcgcca gcttcgacgt tccccaaccg ctttctgctg
     1561 cagccatcgt cgccaccata ctggtaggga taatgtctac gcgttcaagc aaggtattcc
     1621 acgggatttt tcgcgtatta ccagaccact tcaccttgta gcgtttggcg atgtctttca
     1681 gacgcgcacg ctcagcaggt gaccattggc cctgatactg ttttgaaatc agccagttac
     1741 gttctgcagt aatggctgca ttttggctgg taatgtaagg cattacggtc cggagaaacg
     1801 cctttttcct tggtgttccg gaagggtatt ttcgcaaatc aggaagtgaa ctactctttg
     1861 cactattgcg agaatactct tgtttactgc ttacctgttt attactagct ttagttaagt
     1921 gggacttttg actcgctgtt gttgtgtgcg tcttcgctaa cacctcactc gaaaacacca
     1981 gagtgagtaa cataagaatc atcgccccat atcgtcgtat gggagtcaaa atcatcaggt
     2041 ctcctggtcg gatttaatca ttccaacacc ttatattttt cacaaatttg agagttgaat
     2101 ctcaaatcat atcaaaaata gctgtcaaga gcaccccaag gaatagtcca aatctgaaac
     2161 tatgtcacga gttaacgatt cagattggcg ctaaatcgca gaaaatgtgg gggttatcgc
     2221 aaaattcagc cgttttttgc gcgcgatcgc tcacccttgc ttctcatcct gtggacttac
     2281 cgctcaggga tgagttttgt ctggcttatc gctggcaaac tgtctgaaat cgcagcaata
     2341 aggactcatc caccatgaaa ctcgccgcct gttttctgac actccttcct ggcttcgccg
     2401 ttgccgccag ctggacttct ccggggttcc ctgcctttag cgaacaggga acgggaacat
     2461 ttgtcagcca cgcgcagttg cccaaaggta cgcgtccact cacgctaaat tttgaccaac
     2521 agtgctggca gcctgcagat gcgataaaac tcaatcagat gctttccctg caaccttgta
     2581 gcaacacgcc gcctcaatgg cgattgttca gggacggcaa atatacgctg caaatagaca
     2641 cccgctccgg tacgccaaca ttgatgattt ccatccagaa cgccgccgaa ccggtagcaa
     2701 acctggtccg tgaatgcccg aaatgggatg gattaccgct cacgctggat gtcagcgcca
     2761 ctttcccgga aggagccgcc gtacgggatt attacagcca gcaaattgcg atagtgaaga
     2821 acggtcaaat aacgttacaa cccgctgcta ccagcaacgg tttactcctg ctggaacggg
     2881 cagaaactga cacatccgcc cctttcgact ggcataacgc cacggtttac tttgtgctga
     2941 cagatcgttt cgaaaacggc gatcccagta atgaccagag ttacggacgt cataaagacg
     3001 gtatggcgga aattggcact tttcacggcg gcgatttacg cggcctgacc aacaaactgg
     3061 attacctcca gcagttgggc gttaatgctt tatggataag cgccccattt gagcaaattc
     3121 acggctgggt cggcggcggt acaaaaggcg atttcccgca ttatgcctac cacggttatt
     3181 acacacagga ctggacgaat cttgatgcca atatgggcaa cgaagccgat ctacggacgc
     3241 tggttgatag cgcacatcag cgcggtattc gtattctctt tgatgtcgtg atgaaccaca
     3301 ccagctatgc cacgctggcg gatatgcagg agtatcagtt tggcgcgtta tatctttctg
     3361 gtgacgaagt gaaaaaaacg ctgggtgaac gctggagcga ctggaaacct gccgccgggc
     3421 aaacctggca tagctttaac gattacatta atttcagcga caaaacaggc tgggataaat
     3481 ggtggggaaa aaactggatc agaacggata tcggcgatta cgacaatcct ggattcgacg
     3541 atctcactat gtcgctggcc tttttgccgg atatcaaaac cgaatcaact accgcttctg
     3601 gtctgccggt gttctataaa aacaaaacgg atactcacgc taaagtcatc gaaggcttta
     3661 cacctcgcga ttacttaacc cactggttaa gtcagtgggt ccgcgactat gggattgatg
     3721 gttttcgggt cgataccgcc aaacatgttg agttgcccgc ctggcagcaa ctgaaaaccg
     3781 aagccagctc cgcgcttcgc gaatggaaaa aaactaaccc cgacaaagca ttagatgaca
     3841 aacctttctg gatgaccggt gaagcctggg gccacggcgt gatgcaaagt gactactatc
     3901 gccacggctt cgatgcgatg atcaatttcg attatcagga gcaggcggcg aaagcagtcg
     3961 attgtctggc gcagatggat acgacctggc agcaaatggc ggagaaattg cagggtttca
     4021 acgtgttgag ctacctctcg tcgcatgata cccgtctgtt ccgtgaaggg ggcgacaaag
     4081 cagcagagtt attactatta gcgccaggcg cggtacaaat cttttatggc gatgaatcct
     4141 cgcgtccgtt cggtcctaca ggttctgatc cgctgcaagg tacacgttcg gatatgaact
     4201 ggcaggatgt tagcggtaaa tctgccgcca acgtcgcgca ctggcagaaa atcagccagt
     4261 tccgcgcccg ccatcccgca attggcgcgg gcaaacaaac gacactttcg ctgaagcagg
     4321 gctacggctt tgttcgtgag catggcgacg ataaagtgct ggtcatctgg gctgggcaac
     4381 agtgactttt ccggcttccc gttcgtcagt acctcgggaa gccgccaacc aggataaaat
     4441 gtcagcccta atcagcgttg cgggataaag caccgctcac tcttcaacag accgatttgc
     4501 accccagcaa atgtagcgtt attgttacct tccttgctac agagttcgac agatatcccg
     4561 ctatgacatt ctcccttttt ggtgacaaat ttacccgcca ctccggcatt acgctgctga
     4621 tggaagatct gaacgacggt ttacgcacgc ctggcgcgat tatgctcggc ggcggtaatc
     4681 cggcgcagat cccggaaatg caggactact tccagacgct actgaccgac atgctggaaa
     4741 gtggcaaagc gactgatgca ctgtgtaact acgacggtcc acaggggaaa acggagctac
     4801 tcacactgct tgccggaatg ctgcgcgaga agttgggttg ggatatcgaa ccacagaata
     4861 ttgcactaac aaacggcagc cagagcgcgt ttttctactt atttaacctg tttgccggac
     4921 gccgtgccga tggtcgggtc aaaaaagtgc tgttcccgct ggcaccggaa tacattggct
     4981 acgctgacgc cggactggaa gaagatctgt ttgtctctgc gcgtccgaat attgaactgc
     5041 tgccggaagg ccagtttaaa taccacgtcg attttgagca tctgcatatt ggcgaagaaa
     5101 ccgggatgat ttgcgtctcc cggccgacga atccaacagg caatgtgatt actgacgaag
     5161 agttgctgaa gcttgacgcg ctggcgaatc aacacggcat tccgctggtg attgataacg
     5221 cttatggcgt cccgttcccg ggtatcatct tcagtgaagc gcgcccgcta tggaatccga
     5281 atatcgtgct gtgcatgagt ctttccaagc tggggctgcc tggctcccgc tgcggcatta
     5341 tcatcgccaa tgaaaaaatc atcactgcca tcaccaatat gaacggcatt atcagcctgg
     5401 cacctggcgg tattggtccg gcgatgatgt gtgaaatgat taagcgtaac gatctgctgc
     5461 gcctgtctga aacagtcatc aaaccgtttt actaccagcg tgttcaggaa actatcgcca
     5521 tcattcgccg ctatttaccg gaaaatcgct gcctgattca taaaccggaa ggagccattt
     5581 tcctctggct atggtttaag gatttgccca ttacgaccga gcagctctat cagcgcctga
     5641 aagcacgcgg cgtgctgatg gtgccggggc acaacttctt cccgggactg gataaaccgt
     5701 ggccgcatac gcatcaatgt atgcgcatga actacgtacc agagccggag aaaattgagg
     5761 cgggggtgaa gattctggcg gaagagatag aaagagcctg ggctgaaagt cactaaaaat
     5821 tgcctgatgc gctacgctta tcaggcctac atgatttctg caatatattg aatttggaag
     5881 aatttgtagg ccggataagg cgtttacgcc gcatctggca taaacaaagc gcactattcc
     5941 ggcgttgaga aacgccggaa tacgtttcaa acaggctgct gctgtagccg atgccgctgc
     6001 actttctcga catccatgca ctgcaacgcc tttgtcgggc aggcttcaac acaggccggc
     6061 cccgtctccc gatgccagca cagatcgcac ttaatcgccc tcgccttttt gcgcgaagaa
     6121 acgacctcca tcgcaccaaa cgggcaagcc agcatacagc ttttacagcc aatgcaacgt
     6181 gtttgttcaa cgaaaatatg cccatgttcg cggcttatcg cgtcaacagg gcagacattc
     6241 gcgcacggtg catcttcaca ctgatgacag gctactggcg tggtccagct gtggtcttta
     6301 atgacacgaa tacgggaaat aaactcgtct ggtgacaacg cagcgcaatc ctgattctcc
     6361 tgatgcgaca ctgcgcaagc cacttcacag gtacggcaac cgatacattt cgtcgcatcc
     6421 gcaataataa accggttcat gatcactcct ttgcactaaa acagcagagt gccagagagc
     6481 gcgtccctac tgctttgatc caacgaagga aatgttaaag ttttggcact tgtccaaagc
     6541 gttatgtgat cgcgcccagg tcatccctga cagtaaatcc cagttcatta gaaatagcct
     6601 gcgcggtttc acgcagtggt ttcaggagat ttttctctcc cacctgtttc agacgtgatg
     6661 tcgaaagcga aatcgacacg gcgtacggca cccgcccatg aatatcaaac accggaacag
     6721 caatacagga gacgccgagt tcgttttctt ctctgtccat cgccgctccg ctttcacgaa
     6781 tgtgcgccag ttcgtcgaac atcgcgggca gctcggtaat ggtattgcgg gttaacggct
     6841 ggatctcatg ctgatggttt tcccagtatg acttcacgta gtccgggtga ccaaacgcca
     6901 tgtagatctt gcccattgcg gaacagtaga gcggcatatg ctggccaata taggcacggg
     6961 ttcgcagcat cccggttgtg ggttccagct tataaatcaa aatagcgtga tcgtcttcgc
     7021 ggctggagaa gttaatggtt tcaccagtgg cgatgttcag tgcctcaaga tgcggagcgg
     7081 cgatatgaat gatattcagc gaagacagcg ccttctgccc gacggcaata aatttggtag
     7141 tcaggcggta actccctgcg gcgggcgcgg tggtcacata gccgcaggac tgtaatccct
     7201 gcaataagcg atggacggta ctcttattta aaccagccag ctccgaaaga tgcgccaacg
     7261 gacaaccgtt tggatagttg ctcaaaatct caatcagcat caacccgcga aacagactct
     7321 ggcttccggc tggacgctct ttttcctgcg ccatctcgtt ctcttttttt cccatcactt
     7381 ctttccccat tttgtcgcgt cctgatggta gcgcaaagtg tgccgtagtt cacgatctcg
     7441 acagataatt tataaccact tgatttttat gtcttttgaa attcatcaat cagattgctt
     7501 tgttaaaaag tgatcgatat atttgaaatc tagtttcgta tattgaaatt ttaagccaaa
     7561 aaagcgatca aaaaaacaag gaagcctggg atgaaagtga catttgagca gttaaaagca
     7621 gcctttaatc gggtcttaat ttcacgcggc gttgacagcg aaacggctga cgcctgtgca
     7681 gagatgttcg cccgcaccac cgaatccggc gtttattctc acggcgttaa tcgtttccct
     7741 cgtttcattc aacaactgga aaacggcgat atcattcctg atgcccaacc caaacgtata
     7801 accagcctcg gcgcaattga acagtgggat gcccagcgtt caatcggcaa cctgacggcg
     7861 aaaaagatga tggatcgcgc cattgaactg gctgccgatc acggtattgg tctggtggca
     7921 cttcgtaatg ccaaccactg gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa
     7981 ggctatattg gcatttgctg gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa
     8041 gagtgtcgca tcggcactaa cccgctgatc gtcgccattc cttccacgcc gatcaccatg
     8101 gtcgatatgt cgatgtcgat gttctcttac ggcatgttag aagttaaccg cctggcaggc
     8161 cgtcagctcc cggtcgatgg tggctttgat gatgagggca atttgaccaa agaacctggc
     8221 gttatcgaga agaatcgccg cattttgccg atgggctact ggaaaggttc tggcatgtcg
     8281 attgtgctgg atatgatcgc tactctcctt tccgacggcg catcggttgc cgaagtcacc
     8341 caggacaaca gcgacgaata cggcatttca caaattttta ttgccattga agtggacaag
     8401 cttatcgacg gtcccacccg cgatgccaag ctgcaacgta tcatggatta cgttactacc
     8461 gctgaacgcg ctgacgaaaa ccaggccatc cgcttacccg gccacgaatt tactaccctg
     8521 ctggccgaaa accgccgtaa cggcatcact gttgatgaca gcgtgtgggc caaaatccag
     8581 gcgttatgag gagataagtc atgatttttg gacatatcgc gcagcccaat ccgtgccgtt
     8641 tgcccgccgc cattgaaaag gcgctcgatt ttctgcgcgc taccaacttc aacgtcctgg
     8701 agccgggcgt tgtcgaaatc gacggcaaga atatttatgc gcaaattatc gatttaacca
     8761 cacgcgaagc ggtggaaaac cgtccggaag tgcatcgtcg gtatatcgat attcagtttc
     8821 tggcgtgggg tcaagagaaa atcggcattg ctattgatac aggaaataat aaagtcagcg
     8881 aatcactatt agagcagcgc gatattattt tttatcatga cagtgaacat gaatcattta
     8941 tagaaatgat accgggcagc tacgctattt tcttcccgca ggatgttcac cgaccaggtt
     9001 gtattctgca aacagcctct gagatacgaa aaattgtggt taaagtcgca ttaacagcgc
     9061 tgaattaatc aaataatcgt ctacgccaga acgactgggt aatg
//
LOCUS       DADLSB010000152         9018 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.152, whole genome shotgun sequence.
ACCESSION   DADLSB010000152 DADLSB010000000
VERSION     DADLSB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.152"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(81..1400)
                     /gene="potE"
                     /locus_tag="JN331_003351"
     CDS             complement(81..1400)
                     /gene="potE"
                     /locus_tag="JN331_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY7261412.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSTTLYVDSWNPHHAPFFSTVGSSIAMTLWAFLGLESACANTDVVEN
                     SERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(1397..3584)
                     /gene="speF"
                     /locus_tag="JN331_003352"
                     /pseudo
     CDS             complement(1397..3584)
                     /gene="speF"
                     /locus_tag="JN331_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            complement(3895..3999)
                     /locus_tag="JN331_003353"
     CDS             complement(3895..3999)
                     /locus_tag="JN331_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY7261413.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            complement(4274..4951)
                     /gene="kdpE"
                     /locus_tag="JN331_003354"
     CDS             complement(4274..4951)
                     /gene="kdpE"
                     /locus_tag="JN331_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY7261414.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(4948..7632)
                     /gene="kdbD"
                     /locus_tag="JN331_003355"
     CDS             complement(4948..7632)
                     /gene="kdbD"
                     /locus_tag="JN331_003355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HAY7261415.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFSACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVDNALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            complement(7625..8197)
                     /locus_tag="JN331_003356"
     CDS             complement(7625..8197)
                     /locus_tag="JN331_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY7261416.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            complement(8206..9018)
                     /gene="kdpB"
                     /locus_tag="JN331_003357"
                     /pseudo
     CDS             complement(8206..9018)
                     /gene="kdpB"
                     /locus_tag="JN331_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
BASE COUNT         2235 a         2467 c         2256 g         2060 t
ORIGIN      
        1 cacagccccg aaaaagagcc agtcagttgc ttaactgact ggcattaggt cgcaagatcg
       61 ccctttttta ctttgctttt ttaaccgtgt ttatttttca gttcaaagcg tggtgatacc
      121 agaccataca gtgtccaacc aaggaaggtc acgatggaac cgtacagcat ggcttcttcc
      181 ccggatgagt acagcgcata aaagctatac atcgcgccaa caaaagcaac aaagtttgca
      241 actttcgctt ttgagggggg cacattagcg accttctgaa taatgactaa tgccgccatt
      301 gacagaatat acggaatgat attggtcacc acggccaggt taaccagcac gttgaactga
      361 ctgtttagcg acgggctaat cgtcatcagc gccaacccgc tctggataat cacaatggtc
      421 aacattccct gcaccggtgc atccactttg gttacacggg agaaaatttt agggaagtag
      481 ccttcatcag atgaagattt aaacacctgg gcaatggtga actgccagcc aagcagcgaa
      541 ccgcagcagg acatcaccat cagcgccata atgactttac ccacttctgg tgtgaacatc
      601 tgtgcgaagg ccaggccaaa tggtgccgtt gaatttgcca gctccatatt aggcacaatc
      661 cctgcaatca cgttggtgga gacgatataa atcaccgccg cacctaacgt accgccgagt
      721 accgcgattg gcacattacg ttccgagttt tccactacat cagtattcgc acacgcagac
      781 tccagaccaa gaaaagccca cagcgtcata gcgatggaag aacctactgt actgaagaac
      841 ggtgcatgat gcggattcca ggaatcaacg taaagcgtcg tgctaaacca gaaccagccg
      901 ataatgcaca ggcctaccac cggaataatg accccccaca cggtaatgct actgatttgc
      961 ccggtaatgc gcgcaccacc aaagttagcc acggtacaaa tccacagcac cccgatggtc
     1021 gcaagaccaa tctgcactgg cgacaaactc gcgccgagca gttcggtgcc gtaaccaacc
     1081 gccgaaatag caatcgcgac gttagcaatc agcagtgaga cgccgtaggt atagttcgcc
     1141 ataaagttac cggatttacc gaaagcatac tcggcataac cgcccatacc acctgattta
     1201 cggctgaaca taccgcattt tgcgaatgcc catgccagtg ccattgagcc tacggctgtc
     1261 accagccagg agataattga aattgtccca acttcggcaa gctttgtcgg cagcatgatg
     1321 ataccggagc ccatcatgtt gaccatcgtc agtatggtta actgaacgac gcccattttg
     1381 ttcgatttag cctgactcat aatttttccc ctttcaacag ggtgctttgc gcatcacgag
     1441 gcttgatgac atagcaccaa acttgcttac gaccatcatg ttcttcgata tagacaccct
     1501 gcagctccgg tgcaaaacct ggcagcaggt tgatcccttc ttccagagcg ctgaagtaac
     1561 gcaaaacagc accaccccag atttcacccg gaacaacaca cagcacaccc ggaggataag
     1621 gaagcgcccc ttcggcagcg atacgacctt cggcatccag cagacgaacc agctccactt
     1681 caccgcgtaa ataggcgtag ttggcttctt gcggattcat gctgacgtgt gggaagtgcg
     1741 ctttgcggaa catctctttt tgcagttgtt tcacgttgtg gcgggcatac aaatcatgca
     1801 tttcctggca caactgacgc agggtataac cggcgtagcg ctcttcatgc tgtttgtaga
     1861 tggaaggcag cacttctgct aatggcgcgt cggactcaag cagtttttcg aagcgtacca
     1921 gcagggcaac aagttgctga agtttggcca tatcttctgc cggagtcagc aggaagagga
     1981 tggagttaag atcgcatttt tccggcacta cgcaggaagt tagcaagaat cgtcgcgggt
     2041 acaccgaacg cttcatattc gccgttacgt gcatcaatac ctggggttgt cagcaacagt
     2101 ttgcaaggat cgacaaagta ttgattctct gcatagcctt caaaagaatg ccagtgttcc
     2161 cctggtacaa actggaagaa gcgcagatca accgcaattt gcgctgtttc atacgactgc
     2221 cagggtttac catccaccag ttcaggtacg aacggacgaa tatgctgaca gttatcgagg
     2281 atcagtttgc gggcattaat gccatttacc acacaatcca tccacatatt acgaccgctg
     2341 acaccttcat gcattttggc gttgatatcc agtgcggcaa acagcggata gaacgggctg
     2401 gtggaggcgt gcatcataaa ggcgttgttc atgcgtttgt gcggtacata acgctgttgc
     2461 cctttgatgt ggctgtcttt tttatggatt tgtgaagttt gagaaaaacc agcctgctgt
     2521 ttatgtacgg attgcgtaac cagaataccc ggatcgttct cattaagatc cagcaacagc
     2581 ggcgaacagt cagccatcat cgggataaat tgctcatagc caacccatgc ggagtcaaac
     2641 agaatgtagt cacacagatg accaatttta tccaccacct ggcgggcgtt ataaatcgta
     2701 ccgtcgtagg tgcctaactg aatcacagcg aggcggaaag gacgagcctc ttttgcccgc
     2761 tgcggtgcga cttccgcgat cagctcacgc aggtaacttt cttcaaaaca gtgcgcatca
     2821 atgccaccga taaagccata cgggttacgt gccgtttcca gataaaccgg tgttgcacca
     2881 gcctgtagca acgctccgtg gtggttagat ttgtggttat tgcgatcaaa cagcaccaga
     2941 tcacccggtg ttagcagggc gtttaaaacc actttgttag aagatgaagt gccatttaga
     3001 acgaagtatg ttttatcggc attaaacact tttgccgcat gttgctgtgc aatgcatggc
     3061 gcgccttcgt gaatcagcag atcgcccatt gctacgtctg cgttgcataa gtcggaacgg
     3121 aacagcgttt ctccgaagta ctccacgaac tgattccctg ccggatgacg acggaaaaac
     3181 tcacctccct gatggcccgg gcagtcaaaa gcgctattgc cctgattgac ataatcgacc
     3241 agtgcgcgga agaagggcgg gcgcagttgc gtttcataat ggctagcagc ggtttctaac
     3301 tgacgtccat aaaactcttt ccgtgattcg caatgctcga agacaccaga aatacgtggc
     3361 agatattctg ctggtacacg ttcttcattt tccgttgcaa taaaaacagg aatgccatag
     3421 ccagtagcgt ctattttatc gagttttcca cgttcaacat cattgactga taaaataatg
     3481 gcggcaacgt caatataatt actttcgtca atattcacgc atgcacgatg agtggaaaaa
     3541 caatccgggc aagaacggtt aacggcaatt tttaattctg acattttatt catctcttta
     3601 tttcaggtaa tagcaggtcc tcaatttctc aaaggagaaa tcgatcacgc cggaaaaaaa
     3661 gcaaagctcc accgctgata aataaatcag tagtttgctt tttgcagatg caataaccca
     3721 gaccagcccg gtaactggct atactcgtca tacctcaagt tgcaagtgct gcgactcgaa
     3781 ttatttagag tttaaaaggg ttagcagtat atgagcaccg aggaatacag gtattaacct
     3841 gtaatgctta aagtcaggga gacctgtacg ttggggtgcc ctttagtcag aagactagcg
     3901 ggcattaaag aaatgaaagt caaaactatt acgatgggca aacttcatga tatgggttgt
     3961 ccgccttata tgaggcatta agcggttatt attttccatt tacctttacc gcttgctgtg
     4021 tcagttgaag gtagagttat tctatccatg aaatggctat aatctgtgaa gaagatcaac
     4081 aaaaaacgat cataccccag ataataaatc attaattatg aatatcgcca tattagatgc
     4141 tgttttgtta cttaaatgtt gtaatttcgt gttttatatt tgcgttaaga aattaaacct
     4201 gccaaataat aatattcggc gcaggtttaa taataaatta atcactattt aagcgaattt
     4261 attgaataac aattcaaagc ataaaccgat acccaatacc ggtttcagta atgaagtggc
     4321 gaggccgggc cggatcctgt tccagttttt gtcgcagatg tcccatataa atacgcaaat
     4381 agtgactgtg ttcgaccgcg tttggccccc acacctggtt aaggagctgg cgctgggtga
     4441 gtacttttcc ggcattgttg agcagcaccg ccagcaggcg gaactcaatt ggtgtgagat
     4501 gcacctcttc ctcaccccgg tgaatcacgc gggcggctaa atcgacggta acatcggaaa
     4561 atttcaccag cggatcgggc gcggcggtgg cggagtggcg gcgtaaagcg acgcgcagac
     4621 gggcctgcaa ttcgccaatg ccaaacggct tactcagata atcatccgct ccggcatcca
     4681 gcgcggcgat cttgtcgctc tcttcgctgc gtgcggaaag cacaatcacc ggcaccgggc
     4741 tccactggcg caggtcgcgg ataaactcaa tcccatcacc atcgggcagg ccgagatcga
     4801 gaataatcaa atctggctta cgggttgccg cttccagcaa gccgcgttgc agagtttcgg
     4861 cctcaaagac gcgcatcccg tcgccctcca gcgccgtgcg cagaaagcga cgaatagcct
     4921 gttcatcttc aacaatcaga acgtttgtca catatcctca tgaaagtctt caagttcagg
     4981 agcggtttgc tggggaagtg taacacgaaa acaggcacca ccttccggtc ggttgaacgc
     5041 ggtaatggtg ccgccgtgta catccacaat cgcccgacaa attgccaacc caagcccgac
     5101 acccggcacc gccgactctt tattcccgcg agcaaactta tcaaatatcg tctgctcctg
     5161 gcctggcgga agaccggggc cgttatccca gacatccagt tgtagatttt cgccctcaac
     5221 gtgggcatcg ataccaattt cggcctgcgc acccgcatat ttcaccgcgt tctccagcag
     5281 attaatcagc acccgttcaa agagtggccc gtcaacgtgg attaaggtca gcggttctgg
     5341 cagagaaaga ttgatgggcg acgataaacc cggttccagc atttgcagcg cgctgccgac
     5401 tacttcttcc agcgttaacc actctttctt caaattaaag ccgccggact gaattcgcgc
     5461 catatccagt agattattca ccagtcgggt agtgttcagc acatgctgac gaatctcgct
     5521 ggcctggcgg gcgtggggtg atccttcgct tgccagatcg agcgttaaga tttctgcctg
     5581 accaaacagc accgtaagcg gcgtgcgtaa atcatgcgaa agcgctgcca gcagggcgtt
     5641 gcggatctgt tcacgttcgc ttgccatccg cgcctgttct tcgctggcgg ttagcgtcag
     5701 ccgctcaagg gcattgtcga ctaacagcgt aaacgtctcc agcaggcgct gctgttccgg
     5761 gatcatcaac tggcgcagat tccccggctc caccaccacc agcccgtagg ttttctcgcc
     5821 gctttttagc ggcaaaatct ggtacggtac accgggtaac gtgtcggtgc ccgcgcccgc
     5881 aggcaggcct ttatcaaaac tccactgcgc gatggcatcg tcccatggcg tcattccttg
     5941 cggatgtgtt aacggctgca atttaccgtt gtcatcgggc aacaacacct gactgcgggc
     6001 atgaaacgtg gaggcaataa attgttcgct ggtggcggcg atgtcctgcg gactgcggcc
     6061 caccgccaga gctttcgaca tttcatataa gtgccgtgtg cgttgctcgc ggtaacgggc
     6121 tacccgcgcc tgataacgca cgccagcagt aaggttcccg atcaccagcc cgacggttaa
     6181 catcaccgcg aaggtcagca gatattgcac atcagagacg gcgagcgtgc cgcgtggggc
     6241 gataaaaaag agatcgaaac tcaccacatt aatgacggtg gcaaccaccg aaggccagcg
     6301 tccataaaat agcgccacca ccaccacgcc aagcagatac agcatcacca ggttggcggc
     6361 atcaaacgcc atcagccact gcatggcaat taaggtgata acggcgcata acgcggcggc
     6421 aaccacgcat ccctgaatct gtacccgcca cttgtcttta aatgagcggc tatccggcgc
     6481 gttgttaatc gtgcgggcgg gtggttcatc aagcgcgacc agcacctgat cgagatcggg
     6541 ggcgatgcgc gccagtcggt cagcaaatgt ttcccgacgc caccagcggc gcgaggctgg
     6601 gcgaccgaga ataatcttgc cgagattatg ttcacgggca taacgcacta ccgctttctc
     6661 ttccgctgga tcagaaagtg ttgccgtctc cgcgcccagc tcctgcgcca gacgtaaggc
     6721 gctgagaatt gcccgacgtt ttttctccgg taagcgatgc agggcagggg tttcaacata
     6781 caccgcgtgc cagacgctcc ccagccgtga cgccagccgc gccgccgcgc ggaccagttt
     6841 ttcgctgccg gtgttatggc cgatgcataa aaggatcgcg tcgcgcgtgt gccagacttt
     6901 ctcttcgcca ggatgccccc gccaggcgcg catttgctca tcaacgcgat cggcagtacg
     6961 gcgcagtgcc agttcgcgca gggcgatcag attgcctttg cggaaaaaat gttcaatggc
     7021 gcgctccgcc tgcccggcaa tatagacttt gccttctttc agccgctggc gcagatcgtc
     7081 cgggggcaag tccaccagca ccacgtcgtc ggcggcatcg aaaaaaggat cgggcacggt
     7141 ttcccgtacc tgaattccgg tgacgccgct gaccacatca ttcagacttt ccagatgctg
     7201 aacgttgacg gttgtgaaaa catcaatgcc agcttccagc agttcttcga tatcctgcca
     7261 gcgtttagga tgacgagaac ctggcgcatt actgtgcgcc agttcgtcca ttaagatcag
     7321 cgccggacgg cgggcgaggg cggcatcgag atcaaactcg ctgatatgcc gcccacggta
     7381 cgcctggcgt ttcagaggca gaacagccag cccttcaagc atggcggcgg tatctttccg
     7441 cccatgggtt tctaccacac caaccacaat atccagccct tgcgcccgca gtcgctgggc
     7501 ttctgccagc atcgcccagg tcttcccgac gcctgcacag gcactgaaga aaactttcag
     7561 cttcccccga tgcggcgcgg cagtttgttc cagcagacgg tcggggtcgg gacgtaaggg
     7621 ttcgttattc atcaagctta tccagtgcca gattgagttc gacaatgttg acgaccggct
     7681 ggccgatata tttcaccagc ggttgttggc tgtattttgc gatcagctgc gtgagctgtt
     7741 caacgctgag attacgcgct tttgcgatgc gtgggatctg ccaggccgcc gcttgcgggg
     7801 tgatgttatt gtccagtccg cttgccgacg ccgtcaccag ttcaaccgga acgttcgtgc
     7861 tggcgtccgg gttagcggcc cgtaatgcgg caacgcgtgc ggctatctgt ttatccagct
     7921 ccgggttact gactgccaga ttgctcccgc cagaagcctg tggattatag ggcatttctg
     7981 ctgttgccga cgggcgacca tgaaaatagc cgttgccggt aaaattttgc ccgattaatg
     8041 ccgaaccgcg caccgtatca ccttcacgaa tcaacgaacc gttggcctgc cagggatacc
     8101 accattgccc cagtgcggtg gtcagcagcg ggtaaacgcc gccagtaatc aataacagaa
     8161 agagaaatgt cgataatgcc ggacgtaatc cactcatggt aaacctcaca ccagactaca
     8221 aacggttagc agtaaatcaa tgactttgat accaataaac ggcaccagca gcccacccag
     8281 accgtaaatc cataagttac ggcgcaacat ggcagaagcg gtaagcggtt tataactcac
     8341 gccttttaac gccagaggaa tcaaaaagac gataatcaag gcgttgaaaa tgaccgcact
     8401 gagaatggcg gagtcgggcg aatgcaggcg catgatgttc agcgcattta actgcggata
     8461 tgttgccgcg aacgccgccg gaataatggc gaagtatttc gccacatcgt tggcaatgct
     8521 gaaggtggtc agcgagccac gggtcatcag catctgtttg ccaatgtgca ccacctcgat
     8581 caacttggtc gggttagagt cgagatccac catattgccc gcctctttcg ccgcctgggt
     8641 gccggagttc atcgccaccg cgacatctgc ctgcgccagc gccggagcat cgttggtgcc
     8701 gtcgccggtc atcgctacca aacgaccttc cgcctgatac tgacggatca atgccagctt
     8761 ggcctccggt gtcgcttcgg cgagaaaatc atcgacaccc gcttccgcag ctatcgcggc
     8821 ggcagtcaga cggttatcgc cggtaatcat caccgtttta atgcccattt gcgcagctgg
     8881 gcgaagcgct ctttaatacc gcctttgacg atatctttca gcgcaataac gcccagcaca
     8941 cgagaacctt ccaccaccac cagcggcgtg gctccttgac gcgcaacctg atcgactttt
     9001 tgatcaacat cggcaggg
//
LOCUS       DADLSB010000153         8965 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.153, whole genome shotgun sequence.
ACCESSION   DADLSB010000153 DADLSB010000000
VERSION     DADLSB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.153"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(132..1733)
                     /gene="tap"
                     /locus_tag="JN331_003358"
     CDS             complement(132..1733)
                     /gene="tap"
                     /locus_tag="JN331_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HAY7261417.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGILTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLERPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQESATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(1779..3440)
                     /gene="tar"
                     /locus_tag="JN331_003359"
     CDS             complement(1779..3440)
                     /gene="tar"
                     /locus_tag="JN331_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HAY7261418.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(3585..4088)
                     /gene="cheW"
                     /locus_tag="JN331_003360"
     CDS             complement(3585..4088)
                     /gene="cheW"
                     /locus_tag="JN331_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAY7261419.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(4109..6073)
                     /gene="cheA"
                     /locus_tag="JN331_003361"
     CDS             complement(4109..6073)
                     /gene="cheA"
                     /locus_tag="JN331_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAY7261420.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSTEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFALGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(6078..7004)
                     /gene="motB"
                     /locus_tag="JN331_003362"
     CDS             complement(6078..7004)
                     /gene="motB"
                     /locus_tag="JN331_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HAY7261421.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPELRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVRVPTMPSAEPR"
     gene            complement(7025..7888)
                     /gene="motA"
                     /locus_tag="JN331_003363"
     CDS             complement(7025..7888)
                     /gene="motA"
                     /locus_tag="JN331_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAY7261422.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSGETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQ"
     gene            complement(8015..8593)
                     /gene="flhC"
                     /locus_tag="JN331_003364"
     CDS             complement(8015..8593)
                     /gene="flhC"
                     /locus_tag="JN331_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAY7261423.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(8596..8946)
                     /gene="flhD"
                     /locus_tag="JN331_003365"
                     /pseudo
     CDS             complement(8596..8946)
                     /gene="flhD"
                     /locus_tag="JN331_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
BASE COUNT         2036 a         2475 c         2209 g         2245 t
ORIGIN      
        1 ctattgccag tacacctcag acttatccgc cgaaaatgcg cgtcggaaag cgccaggcgc
       61 tcggtcatct gtaacaataa agacgtttgc ccacagggca gagatgaagt catagcgcct
      121 tctcaatcac ttcaggatac cactggcgca atttgtaact gcgccgactc atgtcgtgct
      181 acttcatgtt cttcaagggt aaataccgcc acacgcgacg aaagatggtc ggcctgattc
      241 gccagttgtt ccgttgccac cgccgcttct tctaccagcg aggagttctg ctgagtcacc
      301 tgatccatct ggctgacagc ctgtgcaacc tgctcaatcc cccgccgttg ttcttccgac
      361 gccgaagcaa tttctcccat aatgtcgttc acgcgggtca ccgaactgac aatatcggtc
      421 atggtcgcgg cggcgttatt caccagtttc gaaccctgct ggacacgatt gactgactct
      481 tcgatcagcc ctttgatctc ttttgctgcc tgcgcgctac ggctggcaag attgcgcact
      541 tcacctgcca ctaccgcaaa accacgcccc tgctctccgg cgcgagccgc ttccactgcc
      601 gcattcaggg ccagaatatt ggtctggaaa gcaattccgt cgataacgct gataatgtcg
      661 ccaatttttt gcgagctggt ggcgctctcc tgcatggtgt gagtcatggt actgacctga
      721 acaccgcccg cctgcgccgt tgtcgcggca ttttttgcca gttccgacgc ctgtcgtgcg
      781 ttatcggcgt tttgccctac cgtggcggtt aattgctcca tactggcggc cgtttgtgcc
      841 agcgatgccg cctgctgttc ggtacgactt gagagatcgt tattgcctgc gacaatctcc
      901 gcgatcccaa tgtgcatctc atggcttccc ttacgcacat cactcaccgt cccacgcaaa
      961 gcctgctgca tggtcttcag gctggcaaaa atggcggtga tctcattacg accatatacc
     1021 gcaatcggac gctccagatt acccgcagca atgctgtcaa aatggctacc gataatggcc
     1081 agtggttgaa caatcatctt gcgcgtccac cacagcgcac tgctgatgta gatcgccgca
     1141 acaataatca tgctgataaa caccagcgcc gaaatctgat agttacgctg gctttgcgca
     1201 ctggcggctt ccagcacatg gttgatctcc agctgccagg cctcaaagtt aacgtcaaac
     1261 gcattctgtg atccctgcac cggcgcagtg aggaaatccg aaagttgatt actttcaagc
     1321 caggtcgcct gatgttcgag atcgttgtgc cagcgggcaa aacttttctc cgtctctttt
     1381 tgcaatgccc tgacgtgctc gttgcccgca gtcatcgcca taaaactttt aaacagcgtg
     1441 gtggattgcg tcagactggc gcgcgccgtc gtcatcaacg ttttaatgtc atccgccgga
     1501 tagctaagcg ctgtcagaat gcctgctttg ttcagcgcgg tactggcctg taacattacc
     1561 gcccgagttt gcgctaatgc cgcacgttgc tgattgctct gctcgccgtg attcagtcgt
     1621 tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac tgccaatctg caagatcccg
     1681 cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa tacgattaaa catcaacgct
     1741 ccccatcagg cggcaatgac cgcgttagta aaaactcgtc aaaatgtttc ccagtttgga
     1801 tcttgttcag taattcgcag tcgtggctgt gccggtggtt gctcactggc aggacgggat
     1861 ggtgtttgcg gtttattggt gagtgggctg gctgccagac ggaacgcgga aaccgcttgc
     1921 gttaaacgac tcgcctgttc ttccagcgca gctgcggcgg cagctgattc ctgcaccagc
     1981 gatgcgttct gttgcgtgac gcgatccatt tccgaaaccg ccaatgcgac ttgatcgatg
     2041 ccacggctct gttcatccga tgccgatgca atctcgccca taatgtcagt cacgcgagtg
     2101 acagcattga cgatattgtt cattgtttcc ccggcgcttt cgaccagcac cgaaccggta
     2161 tcaacgcgcg agacggagtc ttcaatgagg gctttgatct cttttgccgc ctgggcgctg
     2221 cgactggcaa gattacgcac ttcacccgcc accacggcaa aaccacggcc ctgttcaccc
     2281 gcacgcgcgg cttcaaccgc ggcattcagc gcgaggatat tagtctggaa ggcaataccg
     2341 tcgataacgc tgataatgtc ggcaattttc ttcgaactat cggcgatctc atgcatcgtt
     2401 ttcactacgc catccaccac tttgccgccg tgctgggcgg tgtcggaggc actttgcgcc
     2461 agttgcgagg cctggcgggc gttatcggcg ttttgcttca ctgtcgcggt gagctgctcc
     2521 atgctggcgg cagtttcttc cagcgcggat gcctgctgtt cagtacggga ggaaagatcg
     2581 gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg catctgaacc ttcgcggaca
     2641 tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa cgctctgcgc caggtcaccc
     2701 atttcactgc gcccgtcaat ggtcagggta ttcgccaggt taccaccggc gatttcgcga
     2761 atgtgagcaa taatttttgc cagcggagta agcaacatac ggcgaatgcc gtaccacgcc
     2821 accagcagaa tcaataccac caccagcgcg ataaccgcca gttgccactg ggcaaatcgg
     2881 taatcatctg cgttgtcagt gacgatatcg cgatacagtt tttcactgct gagggcgtac
     2941 tgagcaaacg cttcgcccat tgcattttgc attccctggg ttggctgagc gaaataagct
     3001 ccagtattgc cataatcaag ataatcaatc agttcagtta acgctgtgta atagttttta
     3061 tatttttcat caaaattacg gctggtagcg accatttcag gtaacggtgc catgcttttg
     3121 aattttttat aatgcgtcgc ggcctgggcc aatgttttcc tggcgctatc gagcaattca
     3181 actttggcgt tactttgttg attggaggaa tccatcatca tccgcaccgc tgaacgactc
     3241 aggttaatgc gcgtttgcag cattaaatcc caggttgacg tcagctcgcc ctgctgttcc
     3301 cgtaattgat tggaaaccac aaagctcttc tggctatggt gaagggaaga aaaaaacaaa
     3361 ctgccggaaa taagctgtaa cagtgcgaat acccccagca ccattaccaa cagcgtgact
     3421 acgcggatac ggttaatcat aaggcacctt cctgaaaaca agttgatctc gttatcggca
     3481 agaagggggg aaactttatt gctgatgcca cccgccgcga aattgaaata aaaaacccga
     3541 tgcgcagatc atcgggttca tttcaattga ggaaatcggg agaattacgc cacttctgac
     3601 gccgcgctat ctaacagcgc catctcttcg ctgttcagca gtttttcgat gttcaccaga
     3661 atcaacatac ggtcgcccag tgcgcccagt ccagtgagat attctgttga aagcgtcacg
     3721 gcaaactctg gtgccggacg aatttgctcc gccgtcaatg aaagcacgtc tgaaacgccg
     3781 tcaaccacga tgccgaccac ccgctgtccg agattcagga cgataactac cgtgttgtcg
     3841 ttatagtcca catccacctg gctgaactta attcgtaagt caacaatcgg cacaataacg
     3901 ccgcgcagat tcgtgacgcc tttgataaac gctggcgtgt tcgcaatccg tgttacctga
     3961 tcgtagccac ggatctcctg cactttcagg atatcaatac cgtactcttc atcaccaagg
     4021 gtaaatacca gaaattcctg gcctgacggc tcgctggcca gctttgttac attcgtcata
     4081 ccggtcatat tgttaccttt ttactcattc aggcggcggt gttcgccata cgttgttcgc
     4141 ggtttatcgc ctgcaaggcg gagacatcaa caatcagtgc cacgctgccg tcgccaagaa
     4201 tggtcgcagc agaaatgccg gggactttgc gatagttact ttcaaggttt ttaaccacaa
     4261 cctggtgttg accaattaat tgatccacca gcaaggcgta gcggcgaccg ccactttgta
     4321 agatcaccac aattccctgg gtggcttcgg ttttcgcgcc cgcgacgttg aacactttcc
     4381 acagttcgac gatgggcaga tattcacccc gcacttccag cacccgctcg ccgccggcca
     4441 gtggatggag atcggcttca cggggttgca gtgattccat aacagcattc agcggcagaa
     4501 tgaaaacttc atccgcaacg cgtacggaca tgccgtcgag gatggccagc gtcagcggca
     4561 gtaaaatgcg gatcgtagtg ccagtaccct gcttcgactg gatttcgaca tgaccgccca
     4621 tctcctggat attacgttta acgacgtcca tgccgacgcc gcgcccggag acgtcggtga
     4681 cctgctctgc cgtggagaag cctagtgcaa atatcagcat tgcaacttcg tcgtcgctca
     4741 tgttttcgct gacagtcaaa ccttgcgagg ccgcttttgc cagaattcgc tcacggttta
     4801 gccccgcccc atcgtcggtc acttcaatgc aaatgttgcc gccctgatgt tcggtagaca
     4861 gaattaaatt tccgacgctg tttttacctg cggcgagccg tttttctggc agttcaatac
     4921 cgtgatcgag gctattgcgt accaggtggg tcagcgggtc gataatgcgt tctatcaggc
     4981 tcttgtcgag ctcggtggaa ctgcccacca gcgtcagttc tacctgcttg ccgagttttc
     5041 ccgccagatc acgcaccagc cggggatagc gactaaagac atattccatc ggcatcatac
     5101 gaatcgacat cactgattcc tgcaaatcac gggcgttacg ttgtaactgc cccatgctgg
     5161 taatcaaatc accatgatta accgggtcca gttcgctgga acgctgggca agcatggact
     5221 gggtgataac cagctcgccg acgaggttaa ttaattgatc aaccttttct accgctacac
     5281 ggatgctggt ggattcactg ctgcgcgtcg ttttttcccg ctccacgcgg ccggttggcg
     5341 cttgttcggc tgccagttta agcacaggtg gggtagctat ttttggcgaa acttctactg
     5401 tttcaaaggt aatctgatcg gcttcaatca caaaacagag taccgctgtg atgtcatctt
     5461 cggcgatatc gcccggtaat attgccgaga gagaatccgc ccctttcacc acgtcattta
     5521 acgttgtcag atgccccagc tcttcttcca gcaggtcgac ttcgccggcc ttcaggcgcg
     5581 aaaggataat tcgtcgcggc aactgactgc gactttgctc atcttgcggt tcacttttgg
     5641 caaccacact taatcgggtc actgcggatg gcgtttcgcc tttcgcttct aatgccagtt
     5701 gacgcaaggc ctggcagata taatcgtagc tggcggcatc cggctcttgc gactgtttat
     5761 aagcgtcgag ctgttcttgc atgatgtcct tcgtttccaa aaacagattg ataatgtcgg
     5821 tgttgagttg catctcacct cgtctggctt catcgagcag gttttccatc agatgcgtgg
     5881 tttcctgcaa aacgctgaag ccaaaagttc ctgcccctcc tttgatcgag tgggcagccc
     5941 gaaagatggc attcaattgt tcggcatctg gcgcttccgg ctgcaaaacc agcagatgct
     6001 gctccatatc agccaacagt tcgtccgctt catcaaaaaa tgtctgataa aaatcgctta
     6061 tatcgatgct cacgctgtca cctcggttcg gctgatggca ttgtgggaac tctgacctgt
     6121 ggtgcaacct caggtttttc cagggcgctt actggctcat tctggctttc ggcgttttca
     6181 tgcaaaatgg cctgttcggc ttgtttgttc agtaccagca ggctgatgcg acggttgacg
     6241 gcatcatcag gtccgcgatc gcttaagcgc atcgttgccg ccatgccgac gacacgtaac
     6301 actttgccgc tatccaaccc tccgaccatc agttcgcggc gggatgcatt ggcccgatcg
     6361 gcagaaagct cccagttgct gtaacctttc tcaccgctgg cgtagggaaa atcatcggta
     6421 tgacctgaaa ggctaatacg gttgggaata ccgttcagca caggcgcgat ggcgcgcaga
     6481 atgtcgcgca tatagggttc gacatcggca ctgccggttc taaacatcgg gcgattctgg
     6541 ctatcgatga tctgaatacg tagaccttcc tggaccagat cgattttgag atggagacgt
     6601 aacgcccgca gttccggatc ggactctatc aactggtcga gatcaccccg caatttccgc
     6661 aatcgacttt gctccatgcg ttttttcagc tcttcgatgt tcggctgctt attcacttcc
     6721 ccctggcttt gggtgtaatc atcaccaccg ccgggaattg ggctttcact attggaaatg
     6781 cgatcgccgc ccgtaaccgc tgtcgccagt ggagtccgga agtactccgc aatctgaatc
     6841 aactcttttg ggctggagat ggagatcagc cacatcacca gaaaaaaggc catcatcgca
     6901 gtcataaagt cggcataagc aatcttccac gatccatgtg ctgccccgtg gcttttggct
     6961 ttgcgtcgtt tgacgacaat aatcggatgc gcttgattct tcatgcttcc tcggttgtcg
     7021 tctgttattg cggatttttc accgcacgca catgctcttc cagttcaata aacgacggac
     7081 gttcgctgga atagagcgtt ttgcgaccaa actcaacggc gataggcggt gcgtaaccgt
     7141 tcagattaga aagcagagtg actttgacgc actgcatcat tttgctggtt tcgccgcttt
     7201 tctgacgtaa aacactcgct agtggggaaa taaatccgta agccaataaa ataccgagga
     7261 aagtccccac catcgcatgt gcgataagcg cccccagctc ggcggcagga cgatcggctg
     7321 aacctaacgc gtgaacgccc cccattacag ccgcgacaat accaaacgcc ggaagtgagt
     7381 ccccgaccag cgccagactg ttcgccggga cttctgcctc gctttcgtgc gtctcaatct
     7441 cttcatccat cagggcttcg atttcgaagg tgttcatgtg accgctgata atcaggcgca
     7501 gataatcgac gataaaatca agcatgacgc tatccgcgag gatgcgtggg tagctggcga
     7561 agatctcgct ctcacgggga ttttcaatat cacgttccag cgaaaacatc cccatctgcc
     7621 gcgatttcgc catcaaccga taaagcagag ccagcagatc catatacatt gctttggtgt
     7681 atttggagcg acgaaacagc aacggcagcg ctttcagcgt gcctttaatc gctttgccat
     7741 tattgccgac gataaacgac ccaatccctg caccggcaat aatcaccagt tcagcgggtt
     7801 gatagagtgc tccaaggctt ccaccggtca tcaaataacc gccgaaaact gtaccgagaa
     7861 caaccaggta acctaataag ataagcacga catcatcctt ccactgttga ccatgacagg
     7921 atgttcagtc gtcaggcgtt aacgcgcgat tggggcaaaa aaaagcagcg gtacgtcgtt
     7981 accgctgctg gaatgttgcg ccacaccgta tcagttaaac agcctgtact ctctgttcat
     8041 ccagcagttg tgggataata tcggcaggat tctgggaaag tttacgtctt tttactgccc
     8101 gggatggcgg ttgacataag ctgcaggcaa agctgccaac aggctggtga gcgtgggtaa
     8161 taaaattgcc gccgcagcag ttgcagctgg aaagttgcag taatccactt tcaacaaacc
     8221 gcaccaatgt ccaggcacgg gttaatgcca gcagtggtcc ttcttctgct tgtgggcact
     8281 gttcaaggta taaacggtag gctttgatca ccgcatcgac gccattacac aaaccggttt
     8341 tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg aacgttttgt tcccaggtca
     8401 taaaccagtc ggttgaaaat ggcagcatgc ctttcggcgg tgggcttccg cgcagttctt
     8461 tataaagttt tatcaggcgt ccgcgactta actgtgtttc gctttccagc atctgcaaac
     8521 gagcgcccag ggtgatcaat tccattgcca gctgaatatc ccgcgcttcc tgaacaatgc
     8581 ttttttcact catgatcagg cccttttctt gcgcagcgct tcttcaggct gattaacatc
     8641 attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt tgctggagat cgtcaacgcg
     8701 ggaatcttgc gtcaactgag taatcgtctg gtggctgtca aaacggaagt gacaaaccag
     8761 ctgattggtt tctgccagct taaccatttg cggaagagtc agtgccgcta acgttgtcgc
     8821 catttcttca tttatgccga gacgaaacat agcggacgct ttgtcctaaa caatcagacg
     8881 ctgtgcaagt agtaaatatg acaagttgat gtcataaatg tgtttcagca actcggaggt
     8941 atgcattatt cccacccaga ataac
//
LOCUS       DADLSB010000154         8965 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.154, whole genome shotgun sequence.
ACCESSION   DADLSB010000154 DADLSB010000000
VERSION     DADLSB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.154"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..278
                     /locus_tag="JN331_003366"
     CDS             <1..278
                     /locus_tag="JN331_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY7261424.1"
                     /translation="SPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(287..685)
                     /gene="vapC"
                     /locus_tag="JN331_003367"
     CDS             complement(287..685)
                     /gene="vapC"
                     /locus_tag="JN331_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY7261425.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(685..912)
                     /gene="vapB"
                     /locus_tag="JN331_003368"
     CDS             complement(685..912)
                     /gene="vapB"
                     /locus_tag="JN331_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY7261426.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1066..1413
                     /locus_tag="JN331_003369"
     CDS             1066..1413
                     /locus_tag="JN331_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HAY7261427.1"
                     /translation="MLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSR
                     MQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRHHPCGGTGLHT
                     GDDGRTVRNGADR"
     gene            1400..6271
                     /gene="traI"
                     /locus_tag="JN331_003370"
     CDS             1400..6271
                     /gene="traI"
                     /locus_tag="JN331_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY7261428.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQVIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTI
                     RVGTGDRIRFTKSDRDRGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSTGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            6291..7037
                     /gene="traX"
                     /locus_tag="JN331_003371"
     CDS             6291..7037
                     /gene="traX"
                     /locus_tag="JN331_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY7261429.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMSLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7092..7652
                     /locus_tag="JN331_003372"
     CDS             7092..7652
                     /locus_tag="JN331_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY7261430.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7783..7995
                     /locus_tag="JN331_003373"
     CDS             7783..7995
                     /locus_tag="JN331_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY7261431.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8179..8700
                     /locus_tag="JN331_003374"
     CDS             8179..8700
                     /locus_tag="JN331_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY7261432.1"
                     /translation="MLHYSGGLKYRCHLSDMENNMRKYIPLVLFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQ
                     RDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSD
                     ADPVPPWIWRHRK"
     gene            8746..8955
                     /gene="hha"
                     /locus_tag="JN331_003375"
     CDS             8746..8955
                     /gene="hha"
                     /locus_tag="JN331_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7261433.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
BASE COUNT         2116 a         2229 c         2826 g         1794 t
ORIGIN      
        1 tgtctcctgt catcaacgat aagaagtcag attcaggtgt gaatgttccg gcagggggga
       61 tcgagcagga gctgaaaatg aaaccggaag aagagatgga acagcaactg ccacccggga
      121 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
      181 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      241 aacgcgggga ggatgttgag ccgggagatg atttctgatg agcaggtcag ctccagtctt
      301 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      361 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccttgac
      421 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      481 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      541 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ctcccctggt
      601 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      661 tggtatcgag cataaacttc agcatcagaa tgactccctt tcctgcatgc cgggctgttc
      721 cctgttatcc ataaaatcgg cgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      781 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      841 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      901 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      961 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
     1021 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
     1081 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1141 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1201 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1261 gtctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1321 gtggattttg ctgtccgtca tcacccgtgt ggaggcactg gcctccacac gggtgatgac
     1381 ggacggacag tcagaaacgg tgctgaccgg taatctggtg atggcactgt ttaaccacga
     1441 caccagtcgc gatcaggaac cacagttaca cacgcatgcg gtggtgacta atgttacgca
     1501 gcataacggc gaatggaaga cactgagcag tgacaaagtg gggaaaacgg ggttcattga
     1561 gaatgtgtac gctaatcaga ttgcctttgg caggctctac cgggaaaaac tgaaagagca
     1621 ggttgaggcg ctgggctatg aaactgaagt ggtcggtaaa cacggtatgt gggaaatgcc
     1681 gggtgtaccg gtggaggcct tttccggacg ctcacagact atccgggagg ccgtcgggga
     1741 agacgcctcg ctgaaatccc gggatgtggc ggcgctggat acgcgtaaat ccaaacagca
     1801 cgtcgatcct gaagtcagaa tggccgagtg gatgcagacg ctgaaggaaa ccgggttcga
     1861 catccgggca tatcgtgacg cagcggaaca acgggcatat acccgcacgc agacacccgg
     1921 acccgcttca caggacgggc cggatgtgca gcaggcggtg acacaggcca ttgccggatt
     1981 aagtgaacgc aaagtgcagt ttatgtatac ggacctgctg gccaggacgg tcggcatact
     2041 gccgccggaa aatggtgtga ttgaacgggc acgcgccggt atcgatgagg ccatcagccg
     2101 tgagcagctt atccccctcg atcgtgagaa ggggctgttc acgtccggga ttcatatgct
     2161 cgatgagctg tcagtccggg cactcagtcg tgacatcatg aaacagaacc gggtgaccgt
     2221 acatccggag aaaagtgtcc cccggacggc cggttacagc gatgccgtca gtgttctggc
     2281 acaggaccgt ccgtcgctgg ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga
     2341 gcgggtggct gaactggtca tgatggcccg ggagcagggg cgggaggtgc agattatcgc
     2401 tgctgaccgt cgctcgcaga tgaacctgaa gcaggatgaa cggctgtccg gtgaactgat
     2461 aaccggacgt cgtcagttgc tggaaggcat ggccttcacg ccgggcagta ccgttatcgt
     2521 cgaccagggc gaaaaactct ccctgaaaga gacgttaacc ctgctggatg gtgccgcacg
     2581 tcataacgta caggtcctga taaccgacag cgggcagcga accggtacag gcagtgcact
     2641 gatggccatg aaggatgccg gggtgaacac atatcgctgg caggggggag aacagcgacc
     2701 ggccaccatc atcagtgaac cggaccgtaa tgtccgctat gcccggctgg ccggagattt
     2761 tgcggccagc gtgaaagccg gagaagagag cgtggcacag gtcagcggag tacgggaaca
     2821 ggccatactg acacaggtca ttcgcagtga gctgaaaaca cagggcgtgc tcggacaccc
     2881 ggaggtaacc atgaccgccc tgtcaccggt ctggctggac agccggagcc gttatctgcg
     2941 ggatatgtac cgcccgggaa tggtgatgga gcagtggaac ccggagacac gcagtcatga
     3001 ccgctatgtg acagaacgtg tgacggcgca gagtcacagc ctgaccctgc ggaatgcgca
     3061 gggcgaaacg caggtggtgc gtatttcttc cctggacagc agctggtcgc tgttccggcc
     3121 ggaaaaaatg ccggtggcag acggtgagcg tctgagggtg acagggaaaa ttcccggact
     3181 ccgcgtctcc ggcggtgacc gcctgcaggt ggcatcagtc agtgaagatg cgatgacggt
     3241 tgttgtgccg ggacgggctg aaccggccac cctgcctgtg agcgattcac cgttcacggc
     3301 actgaagctg gagaacggct gggtggaaac gcccgggcat tccgtcagcg acagtgcgac
     3361 ggtttttgcc tccgtcacac agatggcaat ggacaatgcc accctgaacg gtctggcccg
     3421 cagtggtcgt gatgtccggc tgtattcctc actggatgaa acccgtactg cggaaaaact
     3481 tgcccgccat ccctccttta cggtggtttc tgagcagata aaggcgcgtg ccggtgagac
     3541 attgctggaa accgctatca gtctgcagaa agccgggctt cacacgccgg cacagcaggc
     3601 cattcatctg gcccttcctg tgctggaaag taaaaacctg gccttcagca tggtggacct
     3661 gctgacagag gcgaagtcgt ttgctgcaga aggaaccggt tttgctgacc tgggagggga
     3721 aatcaatgcg cagataaaac ggggtgattt actgtatgtg gatgtggcaa aaggctatgg
     3781 cacaggcctg ctggtttccc gtgcgtcgta tgaggcagaa aagagcattc ttcgccatat
     3841 tctcgaaggt aaggaggcgg tcacgccgct gatggagaga gtacctggcg aactcatgga
     3901 gaaactgaca tcaggacagc gcgccgccac ccgtatgata ctggaaacgt ccgaccgttt
     3961 cacggtggtg cagggctatg ccggtgtggg taagaccaca cagttccggg cggtgatgtc
     4021 agccgtgaac atgctgccgg agagtgaacg tccccgtgtc gtggggctgg ggcccacaca
     4081 ccgtgcggtc ggggagatgc gcagcgccgg cgtggatgca cagacactgg cgtcctttct
     4141 gcatgacacg cagctgcagc agcgcagcgg agaaacgccg gatttcagca acacgctgtt
     4201 ccttctcgat gagagctcta tggtgggcaa taccgacatg gcacgggcat acgccctgat
     4261 tgcggccggt ggcggtcggg ctgtggccag cggtgacacg gaccagctgc aggccatcgc
     4321 gcccggtcag cctttccgtc tccagcagac gcgcagtgct gccgatgtgg tcatcatgaa
     4381 ggagattgtg cgtcagacgc cggaactgcg ggaggcggta tacagcctga ttaaccggga
     4441 tgtggaaagg gcgctgtccg ggcttgagcg tgtgaaaccg tctcaggtgc cacgtctgga
     4501 gggcgcatgg gcaccggagc actccgtgac ggagttcagt cacagccagg aagcgaaact
     4561 ggcagaagcg cagcagaagg cgatgctgaa aggcgaggct tttccggatg tccccatgac
     4621 actgtatgaa gccattgtcc gcgactatac cggcaggaca ccggaagcac gggagcagac
     4681 gctgattgtc acgcacctga atgaggaccg gcgcgtactg aacagcatga ttcatgatgc
     4741 acgggaaaag gccggagaac tggggcaggt gcaggtcatg gtgcctgtcc tgaacacagc
     4801 gaatatacgc gacggggagc tgcgtcgtct ctccacctgg gagaataacc cggatgccct
     4861 tgccctggtg gataatgtgt atcaccggat tgccggtatc agcaaggatg acgggctgat
     4921 aaccctgcag gatgcggaag gtaacacgcg gctgatttcg ccccgggagg cggtggctga
     4981 aggcgtcacg ctgtacaccc cggacaccat ccgggtgggg accggtgaca ggatacgctt
     5041 cacgaagagt gaccgggatc gcggttatgt ggccaacagc gtctggacgg tgacagcggt
     5101 ttccggtgac agtgtcacac tttctgacgg tcagcagacc cgtgtgattc gccccggtca
     5161 ggagcgggca gagcaacata ttgacctggc ctatgccatc accgcccacg gtgcacaggg
     5221 ggcgagtgaa acctttgcca tcgcgcttga aggcacggaa ggtaaccgga aactgatggc
     5281 cggctttgag tcagcctacg tggccctgtc gcgtatgaag cagcatgtgc aggtgtacac
     5341 cgataaccgt cagggctgga cggatgccat caacaatgcc gtacagaaag gaactgccca
     5401 cgatgtgttt gagccgaaac cggaccggga ggtcatgaat gcagagcggc tgttcagtac
     5461 ggcgcgggag ctgcgggacg tggcggcagg ccgtgccgtt ctccgtcagg cggggctggc
     5521 cgggggagac agtcctgcac ggtttattgc tccgggacgt aaatatccgc aaccgtatgt
     5581 ggcactgccg gcgtttgacc gtaacggcaa gtccaccggt atctggctga acccgctgac
     5641 cacggatgac ggaaacgggc tgcggggatt cagcggtgaa ggtcgggtaa aaggcagcgg
     5701 ggatgcgcag ttcgtggccc tgcagggcag ccgtaacgga gaaagcctgc tggctgataa
     5761 tatgcaggat ggcgtccgga ttgcccgtga taatcctgac agtggtgtgg tggtaagaat
     5821 cgccggtgaa ggccgtccgt ggaatcccgg tgccatcacc ggtggccgcg tgtgggggga
     5881 tatcccggac aacagcgtcc agccgggagc cggaaatggc gaaccggtca cggcagaggt
     5941 actggcacag cggcaggctg aagaggccat ccgccgtgaa acggaacgtc gcgcagatga
     6001 aattgtccgt aaaatggcag agaacaaacc tgacctgccg gacggcaaaa cagagcaggc
     6061 tgtcagggaa attgccgggc aggagcgtga ccgggctgcc ataactgaac gggaagccgc
     6121 gctgccggag agtgtactgc gtgaaccaca acgggtgcgg gaagcggtcc gggaagttgc
     6181 ccgggaaaat ttgctgcagg agcgtctgca gcagatggag cgggatatgg ttcgtgacct
     6241 gcagaaagag aaaaccccgg gcggagactg atacaggaag acaaacactg atgacaacgg
     6301 ataacacgaa cacgacacgt aacgattcac tggctgcacg gaccgatacc tggttgcagt
     6361 catttctggt ctggtcaccc ggacagcggg atatcatcaa aacggtggca ctggtgctga
     6421 tggtactgga ccacatcaat ctgatattcc agctgaagca ggaatggatg tctctggccg
     6481 ggcgaggagc ctttccgctt tttgccctgg tgtgggggct gaatctgtcc cgtcatgcgc
     6541 atatccggca accggccatt aaccggctgt ggggatgggg aatcattgcc cagttcgcat
     6601 attatctggc aggctttccc tggtatgagg ggaatatcct gtttgccttt gcagtggcgg
     6661 cacaggtgct gacgtggtgt gaaacgcgca gtgggtggcg tacagcagcg gccatactcc
     6721 tgatggccct gtgggggcct ttgtccggca ccagttacgg tattgccggg ttgctgatgc
     6781 tggcagtcag caaccggctg taccgggcgg aagacagagc ggaacgtctg gcgctggtcg
     6841 cctgcctgct ggctgttatt ccggcgctta accttgccac cagtgatgca gcggcggtgg
     6901 ccggtcttgt gatgacggtg ctgaccgttg gtctggtgtt gtgtgccgga aaatcattac
     6961 cccgtttctg gcccggagac tttttcccga cgttctatgc ctgtcatctg gctgtgctgg
     7021 gcgttctggc gctgtgacgg gtgtggtatc tttggccgca agaggatgat ttgtcagagg
     7081 cagaacacag catgacagag cagaagcgac cggtactgac actgaagcgg aagacagagg
     7141 gaacagcgcc tgtccgcagc cggaaaacca tcatcaatgt caccacgcca ccaaagtgga
     7201 aggtgaaaaa gcagaagctg gccgagaaag cggcccgtga agcagagctg gcggcaaaaa
     7261 aagcgcaggc cagacaggcg ctgtccattt atctgaacct gcccacgctg gatgaggctg
     7321 tgaataccct gaagccctgg tggccgggat tatttgacgg tgacacgccc cggcttctgg
     7381 cctgcggtat ccgggacgtg ttactggaag acgtggcgca gcggaatatc ccgctctcgc
     7441 ataaaaaact gcgcagggcg ctgaaggcca tcacccgttc agaaagctat ctgtgtgcca
     7501 tgaaagccgg tgcctgccgg tatgacacgg aagggtatgt gacggagcat atttctcagg
     7561 aggaggaagc gtatgcggga gcgcgtctgg cgaaaattcg tcgccagaac cggataaagg
     7621 cagaacttca ggccgtgctt gatgagaaat aaaaaaagcc tcccccagag agaggaggca
     7681 gggaaataag gcttaaaagg aaataacttc cgcttccgaa aggaagccag tccactgaat
     7741 tatttatcac aataatactg acaactaaag ggggacaaga aaatgaatgg attcagaaac
     7801 agttcacgga acggccaggt ctggcgttac cagcgtgccg gcgggcgggc cgttattctg
     7861 gaagtcagtg gacgctggat ggaagcggca gaagcatggc gacgggctgc ctgtgttgcg
     7921 cctaggacag actggcagca gtttgcccgg aaaagggctg aacactgcca ccggcgctgc
     7981 cggggaaggg tgtaacgaaa aaagcagcct tcattactcc aggagtcaga aggctgaaag
     8041 gagtttatga gatttgcttc ttaatatccg gtcgggcagt atgaccggac agttctgagt
     8101 agcacagagt cttttccgca ttaagtcgat tttttatatt ttgtgattaa tttcacaaaa
     8161 taaggtgtta ttcaatgtgt gctgcactat tcagggggtc tgaaatacag gtgccattta
     8221 tctgatatgg agaataacat gaggaaatat attccactgg tattatttat cttttcatgg
     8281 ccggtattgt ctgcagatat tcatggacgg gttgttcgtg tactggacgg tgataccatc
     8341 gaggtcatgg actcactgaa ggcggtgcgg atcagactgg taaatattga tgcaccggaa
     8401 aagaaacagg attatggccg gtggtcaaca gacatgatga aatcactggt cgcaggaaaa
     8461 acggttaccg tcacatattt tcagcgagat cgttatggtc gtattctcgg gcaggtttat
     8521 gcaccagacg gaatgaatat taatcagttt atggtccggg ccggggctgc ctgggtatat
     8581 gagcagtaca acacagatcc cgtgctacct gtccttcaga acgaagccag acaacaaaaa
     8641 cgtgggctct ggtcagatgc tgatccggtt cccccctgga tatggaggca ccgtaaataa
     8701 agcgatggca atcctatcat tcatctgttc tcagcgggaa taaaaatggc aaaaacaaaa
     8761 caggaatggc tgtatcagtt acggcgatgt tcatcagtca atacgctgga aaaaattatt
     8821 cataaaaaca gagacagttt atcaaacagt gaacgtgaat catttaattc agcagcagac
     8881 caccggctag cggaattaat caccggaaag ttatatgaca ggataccaaa agagatatgg
     8941 aaatatgtaa ggtaataatt cagca
//
LOCUS       DADLSB010000155         8943 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.155, whole genome shotgun sequence.
ACCESSION   DADLSB010000155 DADLSB010000000
VERSION     DADLSB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.155"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(123..1040)
                     /locus_tag="JN331_003376"
     CDS             complement(123..1040)
                     /locus_tag="JN331_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY7261434.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVFMTR"
     gene            1100..1378
                     /locus_tag="JN331_003377"
     CDS             1100..1378
                     /locus_tag="JN331_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261435.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1379..1906)
                     /gene="gadE"
                     /locus_tag="JN331_003378"
     CDS             complement(1379..1906)
                     /gene="gadE"
                     /locus_tag="JN331_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY7261436.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNILEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2705..3277)
                     /gene="hdeD"
                     /locus_tag="JN331_003379"
     CDS             complement(2705..3277)
                     /gene="hdeD"
                     /locus_tag="JN331_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY7261437.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3532..3864
                     /gene="hdeA"
                     /locus_tag="JN331_003380"
     CDS             3532..3864
                     /gene="hdeA"
                     /locus_tag="JN331_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY7261438.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3980..4306
                     /gene="hdeB"
                     /locus_tag="JN331_003381"
     CDS             3980..4306
                     /gene="hdeB"
                     /locus_tag="JN331_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY7261439.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4370..5016
                     /locus_tag="JN331_003382"
                     /pseudo
     CDS             4370..5016
                     /locus_tag="JN331_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5058..5588)
                     /locus_tag="JN331_003383"
     CDS             complement(5058..5588)
                     /locus_tag="JN331_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7261440.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNI
                     KRINELVRHQHIDYLV"
     gene            complement(5744..6310)
                     /gene="slp"
                     /locus_tag="JN331_003384"
     CDS             complement(5744..6310)
                     /gene="slp"
                     /locus_tag="JN331_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY7261441.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6557..7780)
                     /locus_tag="JN331_003385"
     CDS             complement(6557..7780)
                     /locus_tag="JN331_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261442.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSENKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8361..>8943)
                     /locus_tag="JN331_003386"
     CDS             complement(8361..>8943)
                     /locus_tag="JN331_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7261443.1"
                     /translation="FERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIEN
                     PVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHA
                     GADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVI
                     LLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2597 a         1756 c         1853 g         2737 t
ORIGIN      
        1 ttatcgatat cgaagcattg agcattttta actcgtttta tcttgaatgc aatcggcgca
       61 taacttattg aatcccatca aatcccgtct ttttttaccc attcccggtt atcttgaata
      121 aattacctag tcataaatac attctggcgg ctaccttcat ccactaatgc cgtgacatac
      181 atgccaggca gcaagtcacc atttgggttg gggaaaatcg cccgtaacgt cacggagccc
      241 gtggtttcat caaccgtcgg gtcggagaat ttcagcgtgc cggtctggct gtaacgttta
      301 ccattttcca gattgagctg tactggcgta ctgccctgaa cctgtttgat ttgcccactg
      361 gcgacctctt ctttcatgcg taagaaatct tgcaccgact gcgtgagatc gacataaatc
      421 gggtccagac gttgtacggt aaccagcgaa tctgcctgat tagcggtaac gagtgcgccg
      481 acggtcaccg acgatttccc gctgacgccc gtaatcggcg aggtgacatt cgcgtattgc
      541 agattgatcg tcgcctgttc aacagccgct ttggcgacgg tgacattggc ttctgcttca
      601 ttcagctggg tgcgcgcggt gtcgtaatcc tgacggctaa cgtagttggt cttcagcaac
      661 gatgcctggc ggttaaaggt gatgcgggca ttgctggcgg tagagagcgc tttcgccagc
      721 gagcctttgg cggagtttag ctcggcctgt aaaggtgcag gatcaatctg atacagcgaa
      781 tcgccctggt tcactttatc gccttcgata aagttgcgtt taatgataat accgcccacc
      841 tggggacgta tctcggcaac ttcataagga acggttctac cgggcaattc gctcaacaca
      901 ttgaccgaac caggggagag tgtgacgaca ccgacctcag gcgtcatggc ggcggcgttt
      961 tccgccgatt tgtcatcgca ggcggtgagc atcgcgccgc agaataacaa cggtattaac
     1021 agctttcttc ttctgttcat tttagtccct gaaaattctt gagaaataac ggttctttaa
     1081 attgcgcgcg aggagagata tgagggattt acagaccagc ggcatagtgg ggttgtcagc
     1141 cagtaaggtg ggatacaggc acagtgatcg acatggtgag gtcaacgaca aaaaaaatgt
     1201 tgccgttctg ccaacagttc ctgccagcat tcgggcaacg aagggttcta ctggtggata
     1261 cacataccag gggaatcaag tcgttttagc ctctccttta gtaatcaccg ggggtaatga
     1321 aatcagcatc tgcatccctc gtcatgccag ccatcaattt cagttgctta tgtcctgact
     1381 aaaaataaga tgtgataccc agggtgacga tgtcgctcat acgtcttagt ttcagcttgt
     1441 acataatgtt gaatttctga atctttaatg tttgttgcga aatattgaga gtcgacgtga
     1501 tattgctttc atttttattt ttcagaatca gttgcataat tttgaactga ttatgtgaaa
     1561 aacataattg tgaattctta tggggcaagt gtttaccata agtaatctca agaatatttt
     1621 tgatactttc tttgcggcta acaaaataaa taggcgcttt agcttttagt ttactgatgt
     1681 gataaggtgc caaaacaata attctctcgg catctaattt ctccagaaat ttaatcgctt
     1741 cttcatcaag gatatgattg tgataggtat caatgataac cttgaagggt ttattgccta
     1801 cttttttgat ctctgacagg gaattgattt ttatcatttc gtgattatct ttcaactgcc
     1861 aaaagccctg taaaagaaaa gaatctttcg tcatgagaaa aatcataact tgctccttag
     1921 ccgttatcgt ttcgaacatg tcatcctttt cattatttac atccttgtcc gaatcgttgt
     1981 tcaatatagt aaacgcctat aagaactttg cccttcgtga actaatacgt ctattaacac
     2041 taatcaatac ttcagattta tccatcacgc actccatata ttgatcctaa gtaagattcc
     2101 tggttgttat cagcttgtaa atagtaaaca aaaagttaac gtttgcggat tactgcccaa
     2161 gaataagtta tcaataacca gtttgtgatc tctgaagaat attactaaag ttaaaatgta
     2221 atccgattta aatatcgagt ctccttgttt cgacttaagc tggcaattgg attgccagct
     2281 ttcttttaac gcattgaaat tacctgagta aataacgcat tcatgtcttc aggtttttat
     2341 atagcgtctg gagacacgga tatttatgca atgtaatcaa cgccagttgc aaaaaaatga
     2401 acccggatta cttatttaac cgcccatagc atcaacgctt acggaactct ttgttcactt
     2461 tcccacagca cttttaaaac attaaacctg actacggaaa atatcagcca tgataatgtt
     2521 tgaaatggct aatttgccat ggagtgaaaa aaattagatg aaattcagta ggttgaaata
     2581 atcactagca ggtaattatt tcaatgatag tgcgcaattg atctacaaca ctgcgtagcg
     2641 gagagagtat taatcggatc atagtcacat caagtgacta tgatccgggt gacaaccggg
     2701 gtaattattg ctgcttaacg aacaaactgg cgaagctgaa caggctggcg gcgctaaata
     2761 tcagttcaat tcccaccagt gtggaaacca gcgttacaga caccatcggc gttgcaccaa
     2821 ggaatatcca ggcaatgacg atatccagca caccaataac gagctgtagc cagctgcctt
     2881 tcattgaacg ctgacgatac caactcatca ggcgaataac ccctgcaaca cagaacaaac
     2941 cggcaataaa tgccgcaatg gcaaaaatac ccagctccgg tgcgcggatg aagaaatagc
     3001 cgatcaataa ataggcgact gcgacgagga aaccggataa taccggccag aaattatgac
     3061 tgcggttgct gaataacccg acaataagcg caatacccga gcagattaat aatgcaccca
     3121 ctactgtgct taaaatatcg ccagagacga acgggaaact gatacacagc aacccgacga
     3181 taaacagcag cacggcaata aactggattg ctctgcgatg ctttttcagc atctccagat
     3241 caaacttcaa aattgttgcc ttatctatat ataacataga accaccctat aaaattaaga
     3301 agaaaatccc ctgctatcaa tctatgccaa aaacgcgtct aagaatacag tcgatttaat
     3361 aaaaatttcc taattgcagt atctgatgca tctgtaactc attgtattga aataaaaata
     3421 tctgattttg atattttcca tcaacatgac atatacagaa aaccaggtta taacctcagt
     3481 gtcgaaattg attcgtgacg gctctttcac tttatagttg aggatattac gatgaaaaaa
     3541 gtattaggcg ttattcttgg tggtctgctt cttctgccag ttgtgagcaa tgcagcggat
     3601 gcgcaaaaag cagctgataa caaaaaaccg gtcaactcat ggacctgtga agatttcctg
     3661 gctgtggacg aatccttcca gccaactgca gttggttttg ctgaagcgct gaacaacaaa
     3721 gataaaccag aagatgcggt tttagatgtt cagggtattg caaccgtaac cccagctatc
     3781 gttcaggctt gtactcagga taaacaagcc aactttaaag ataaagttaa aggcgaatgg
     3841 gacaaaatta agaaagatat gtaattccgg gaatgcgtta catcgtactt ccttgcatat
     3901 tgaacaggcc ggaatatctt ctttaaaagc agctattcct cctgttcata tataatctct
     3961 atattgaatg ggtcacaaaa tgaatatttc atctctccgt aaagcgttta tttttatggg
     4021 cgctgtagcg gctttgtcac tggtgaacgc acaatctgcg ctggcagcca atgaatccgc
     4081 taaagatatg acctgccagg aatttattga tctgaatcca aaagcaatga ccccggttgc
     4141 atggtggatg ctgcatgaag aaacagtata taaaggtggc gataccgtta ctttaaatga
     4201 aaccgatctc actcaaattc ctaaagtgat cgaatactgt aagaaaaacc cgcagaaaaa
     4261 tttgtatacc ttcaaaaatc aagcatctaa tgacttgccg aattaatgag gtacaagtaa
     4321 aaaggagtag caagttgagc catcttgctg ctcctttttg catttttata tgacagcaga
     4381 atttattata cgtcttatac tcgcggcaat tgcctgtggc gctattggca tggaaaggca
     4441 aatgcgcggc aaaggagcag ggttacgcac acatgtatta attggcatgg gaagcgccct
     4501 gtttatgatt gtttcgaaat atggttttgc tgacgtgctg tctttagatc acgtcggact
     4561 cgaccccagc cgtattgctg cttaggtggt gacgggcgtc gggtttatcg gtgcaggtaa
     4621 cattctcgtt cgtaaccaaa atattgtagg tctgacgacg gcagcggata tctgggtgac
     4681 cgccgcatag gtatggttat tggcagcggt atgtacgaac tgggtattta tggttcagtg
     4741 atgaccttgt tggtgctgga agtcttccat caattaacct tccgcctgat gaacaaaaat
     4801 taccatttac agctgacatt agtgaacggt aatacggtat caatgctcga ctggtttaag
     4861 cagcaaaaaa taaaaactga tttagtttcg ttgcaggaaa atgaagatca tgaagtggtc
     4921 gccattgata tccagcttca tgccacaacg ttgatagaag acctgttgcg attattaaaa
     4981 ggtatggcag gggtgaaagg cgtttcaatt agctaatctg tgtggcagcg tagccagact
     5041 caccgtaagc ctgaaattca caccagataa tcaatatgct gatggcgtac cagctcattg
     5101 atccgcttaa tattgaggat catcatgata ttgtgtttat ggcagtagaa cgtttttagc
     5161 gatattttca gtttctccag aatatcttca gttgtcattt gattcgcaat gcagctcaga
     5221 acctgatgtt gcttgcgcga aagtgacaca ttcgcttcag gcacagagca gtaactgcca
     5281 ttgatgagtg cgacaaaatc aatgatagtg atagtccgcg gaacaaaggt caccggtact
     5341 ttccccagac agcgtttaat attaaagggg gagaaaacaa tgacatggac aggttttaat
     5401 cgttcaattt gagtgagaaa attagaatga aatacattat tcattaatgt atcaataatg
     5461 acgaaggcat tctgatgcaa cattacgtcg atctcttctt cgttctgtat atgaacgaca
     5521 ttacctttac tcagaatgtt tttcatcgcg gtgaagaaca tcgtatccct ggtaattata
     5581 agaaacataa ccgactcctg gtgcggacat taactttata atcagtttgc caaaggtaat
     5641 taacaggaca gggcttataa taataatagt tagcttactg tatattaatt ttatgtaata
     5701 catgatgcag atgctgagaa agcatctgca tctttcggtg gtgttatttg accagctcag
     5761 gtgttacctg actcaccgca ttggtgtagt aaggcgcacc ccagcccggt tccggccaga
     5821 atgcaccata gccgtaatcc cacaggttat aggtggtatt aaccacttct ctcaaatgcc
     5881 acacctggat gccctgcata ttcacttcta ggaagttata cgggacttta ttgataaagc
     5941 ctggttgttc accctgaatg gtaccgagga tggtaacaaa gtgattacga tagttcactg
     6001 gatcaaggaa gccgctttgt ctggcgagca gtcggccctg atagttggct tcaatatcag
     6061 gcttcgcata gctatccaac ggtaatacag cgatttctaa caacgtatcc gttttgccat
     6121 tgataacgtt gataaccttc ccaccaaagc gcgcttgttg accaacatat aaccccggct
     6181 ggttatgaac agcaacaaaa cttttttgaa tatcaggttg gttattgcct ttgatatttt
     6241 gcggaattga actacatgcg gcaagcaaaa atgaaaggct gaggatgagt gcaccttttg
     6301 tcatgttcat gttactatcc ttatcaacaa atattaaaac cataatagat atttctttat
     6361 agttttcatc tgattctgag gtgatatgcc tgatagtaac gtaaacctgt ctgatgttta
     6421 tatcccttct gtttagttca attccgttat tttggggcgt ggtatgagta tccatcgccc
     6481 aataatataa ccctttgttt taaaattaca taaaatctat ttttattcaa tgattataaa
     6541 gtgctaataa tcaatattat ggtattatat tctgataatg atttgataag cgaattcttt
     6601 tgccagatgg tatttctatt tccaccccat aatttatatc tttttcatta ggcagatctc
     6661 tctttaaata aaaggggtat gaaatctttt cgggttgaag tgtctcgtaa tgaaatgcta
     6721 tgaattcatt tttagacatt gttgcccatt tttgaatttt attgtataga gagggtgttg
     6781 taaactcttg aagttgttta taacatattt ttttgaactc ttgcttatat tttaatatgt
     6841 catcaaatga aagattcact acctttgcct ctaagaaatc aattattctc gaagcaattt
     6901 ccgctttcgc gcgtaaagag ttgtcattaa tagttaacaa tgtagaaaac aagccattga
     6961 tttgtttgtc aagatgctgt tcatatttat gtagtttggg gatgggattt tccttgtgtg
     7021 aaaaaatagt gttaaataaa tttgcgtcag tgaaatttat attaagtaga tcttcaaaat
     7081 aaaattttga taaggttagg actagcttgg acatatcttg ccctgtcatg ttgaaatcaa
     7141 taaagtcgca tccagcaagg gcttccatcc tattttcatt tttactgtgc tcaatataca
     7201 gcttcgccat ttttaagtac aagctttgaa tgtctcttat atcagttggt aagtcagctc
     7261 gttgcccagc aaaattttta ttttcggaag aaacggtttg gacactgaat ctttcgggta
     7321 ttccttcttc tacgagaatt ttagttatga ttggattgtt gggttctgga ttaacgacaa
     7381 caatgatttg acatacagaa tccttatcct tttcgtatct aatgttatga atctgattaa
     7441 gtattgattg tgtctgttgc gtaagtggtg tcgaacttat cgtttcaatg ttattaataa
     7501 gacttacaag ttgaaccaca acttttgagt agtctgcatt tgtatatgtg gtgttcgtgt
     7561 ggcatgcatt aatttgttgg agttgttgtt gcgctaaatc aaattgttca gggttgtatc
     7621 cttctccaga gaatgtggaa ttaatgggtt gttgttcttc ccaattatca caaggccact
     7681 tcggtgtgtc tggcgtttta acattatcgg tatttgttgt gcagttataa atatcgccgt
     7741 gatatgtatt tattatacct ggggtaaagt caattgacat aacattcctt attcatcatc
     7801 tgttttatgc taatgttttt aaattggata attagaacag gaatggattt tcttctacac
     7861 acaataaata taatgcgtta tatcttcctg atataattac ctttaaattc cctttcattt
     7921 ccacccggta atatttgtgt ataaaaaaag ggcagtgata gtatcgtaaa ggaaaacgtt
     7981 aacaactatc aaaaagcgct cattttaaaa agttattatg ttctccgata gtgtgtgaag
     8041 taatattttt gtgatgtttt agatgtgtaa taatgaacta ggttaaagtg tatttaaaaa
     8101 tgtgttgtat tagcgactga aatctgcctt ttaaaaagga ggagggtact gagacaaaga
     8161 gtcattgctt ctccgataaa atgtcagaga agcatgatta cgtggttatt gtctgttgat
     8221 gacttgttga ccgagccgtc atcattctca atgcattagt taaatcattt gtgattttta
     8281 aggattatcc tgtctatgat tttctactta gaaggatatg gtactaacta tgcatcttat
     8341 agtttactgc tggcatctat ttaaaatagc aattgagtca aatagcccat caagatagcc
     8401 atacctaata taaccgtcag gaatgcagct atcatcggca ttctgaacat tgatttaagt
     8461 aatatcactt ccgtcaggct tgcgccggca ctgccgatga ttaatgccat taatgctccc
     8521 agacccattc cttttgtcat cagaacagat gccagaggga taaccgcctc agcccggata
     8581 tacagcggaa taccgacaac ggcgcttaat gggatggcga agggattatc tgctcctgca
     8641 tgagcggcaa tccacgctga gggaataaag ccataaataa aagatcctat taatactcct
     8701 aacaaaagat atggcagtac atctttaaac tgttgcaacg catctttggt ggctagtttt
     8761 atgggattaa tacttactac agctttggta ctgcaacagg taactacggg gttctcaatg
     8821 gctttagccg ctggactaca acaactcact tttatgggcg tatatgtcgt tcccggcgaa
     8881 gtttttgctg gagcacaaca atttgctgat gagcttttac tggcaatgat atgacgttca
     8941 aac
//
LOCUS       DADLSB010000156         8868 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.156, whole genome shotgun sequence.
ACCESSION   DADLSB010000156 DADLSB010000000
VERSION     DADLSB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.156"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(35..466)
                     /locus_tag="JN331_003387"
                     /pseudo
     CDS             complement(35..466)
                     /locus_tag="JN331_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            630..1187
                     /locus_tag="JN331_003388"
     CDS             630..1187
                     /locus_tag="JN331_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7261444.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            1370..2230
                     /locus_tag="JN331_003389"
     CDS             1370..2230
                     /locus_tag="JN331_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7261445.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            2440..2979
                     /locus_tag="JN331_003390"
     CDS             2440..2979
                     /locus_tag="JN331_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7261446.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
     gene            complement(2951..3787)
                     /locus_tag="JN331_003391"
     CDS             complement(2951..3787)
                     /locus_tag="JN331_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7261447.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3787..4590)
                     /locus_tag="JN331_003392"
                     /note="aph(3'')-Ib"
     CDS             complement(3787..4590)
                     /locus_tag="JN331_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7261448.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(4651..5466)
                     /gene="sul2"
                     /locus_tag="JN331_003393"
     CDS             complement(4651..5466)
                     /gene="sul2"
                     /locus_tag="JN331_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7261449.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            5490..5567
                     /locus_tag="JN331_003394"
                     /pseudo
     CDS             5490..5567
                     /locus_tag="JN331_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            5796..5972
                     /locus_tag="JN331_003395"
     CDS             5796..5972
                     /locus_tag="JN331_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY7261450.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(6154..7158)
                     /locus_tag="JN331_003396"
     CDS             complement(6154..7158)
                     /locus_tag="JN331_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HAY7261451.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(7237..>8868)
                     /locus_tag="JN331_003397"
     CDS             complement(7237..>8868)
                     /locus_tag="JN331_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAY7261452.1"
                     /translation="VMTDTGIDRRYYELCALSEMKNALRSGDIWVQGSRQFKDFEDYL
                     VPPAKFASLKQASELPLAVATDCNRYLNDRLTLLETQLATVNRMATANELPDAIITES
                     GLKITPLDAAVPDTAQALIDQTAMILPHVKITELLLEVDEWTGFTRHFAHLKSGDPAK
                     DKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHIRDETYGAALADLVNAQF
                     RHPFAEHWGDGTTSSSDGQNFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFHT
                     KVVNVGVRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDL
                     GDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKL
                     GSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFN
                     RLGEIRDRSFEQQRYRASGLNLVTAAVVLWNTVYLERAAHALRGNGHAVDDALLQYLS
                     PLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT         2064 a         2334 c         2460 g         2010 t
ORIGIN      
        1 aaaggctgcc tcatcgctaa ctttgcaaca gtgccaggcc gggtcgctca ttactgagcc
       61 atgcgcgggt gtacagcagg cgcttcaggc ggaaagacca ggggaaatca ccaaattcat
      121 ggtaaccgta atattcctta atcagcgcag tatgctctct gagggtggta acccgttctg
      181 cgtagcgggt aaggacttcc ggacggcgaa tattaagctg caccgcgaca aattgctgaa
      241 cacctggcag aacccgggtt aagtctgtca gaaaggtgcc cagaaaacgg gcggtggtga
      301 gctgaagcgc aattcccagc cggttatgcc ttccccgccg ctggttaatg aaggcaagat
      361 cccgctcatc aagatgaaaa tagcgcgcca gctgcacgtc attgggttct gcaacatagc
      421 gaccataatt cagttcctgc tcagtggtca gaaaatcgac gggcataccg gcctccctgc
      481 ctgatggtca tgaattaaca atttcgcaac cgtccgaaat attataaatt atcggacatc
      541 gtaaaactgt tacattaata tgtctattaa atcgtaaatt tgtaataata gacatgagtt
      601 gtccgatatt cgatttaagg tacattttta tgcgactttt tggttacgct cgggtctcaa
      661 ccagtcagca gtctcttgat cttcaggtca gagcactcaa agacgcaggt gtgaaagcaa
      721 accgtatatt taccgataag gcatccggca gttcaacaga ccgggaaggg ctggatttgc
      781 tgaggatgaa ggtggaggaa ggtgatgtca ttctggttaa gaagctcgac cgtcttggcc
      841 gcgacactgc cgatatgatc caactgataa aggaatttga cgctcagggc gtggcagtcc
      901 ggttcattga tgacgggatc agtaccgacg gtgatatggg gcaaatggtg gtcaccatcc
      961 tgtcggctgt ggcacaggct gaacgccgga ggatcctaga acgcacgaat gagggccgac
     1021 aggaagcaaa gctgaaagga atcaaatttg gccgcaggcg taccgtggac aggaacgtcg
     1081 tgctgacgct tcatcagaag ggcactggtg caacggaaat tgctcatcag ctcagtattg
     1141 cccgctccac ggtttataaa attcttgaag acgaaagggc ctcgtgatac gcctattttt
     1201 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     1261 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1321 gagacaataa ccctggtaaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1381 acattttcgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1441 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1501 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1561 tccaatgatg agcactttta aagttctgct atgtggtgcg gtattatccc gtgttgacgc
     1621 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1681 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1741 cataaccatg agtgataaca ctgctgccaa cttacttctg acaacgatcg gaggaccgaa
     1801 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1861 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     1921 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1981 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2041 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2101 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2161 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2221 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2281 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2341 ttaacgtgag ttttcgttcc actgagcgtc agacccctaa aagaactttc cgctaagcga
     2401 tagactgtat gtaaacacag tattgcaagg acgcggaaca tgcctcatgt ggcggccagg
     2461 acggccagcc gggatcggga tactggtcgt taccagagcc accgacccga gcaaaccctt
     2521 ctctatcaga tcgttgacga gtattacccg gcattcgctg cgcttatggc agagcaggga
     2581 aaggaattgc cgggctatgt gcaacgggaa tttgaagaat ttctccaatg cgggcggctg
     2641 gagcatggct ttctacgggt tcgctgcgag tcttgccacg ccgagcacct ggtcgctttc
     2701 agctgtaagc gtcgcggttt ctgcccgagc tgtggggcgc ggcggatggc cgaaagtgcc
     2761 gccttgctgg ttgatgaagt actgcctgaa caacccatgc gtcagtgggt gttgagcttc
     2821 ccgtttcagc tgcgtttcct gtttggggtc gtttgcggga aggggcggaa tcctacgcta
     2881 aggctttggc cagcgatatt ctccggtgag attgatgtgt tcccagggga taggagaagt
     2941 cgcttgatat ctagtatgac gtctgtcgca cctgcttgat cgcggccgcg atagctagat
     3001 cgcgttgctc ctcttctcca tccgcgttcc aagctgcgga aaggcaccca taagcgtacg
     3061 cctggtcgag caggcgacgc ggatcgacgt ccagcgcacg agagaatgcg tccgccatct
     3121 gtgcaatgcg tctaggatcg agacaaaggt cgtctctgtc agccggatcg tagaacatat
     3181 tggcggcgcc aaagcccact tcaccgacca gaccgacggg atctatcacc agccagccgc
     3241 gactggagaa catgatgttt tcatgatgca gatcgccatg tagcccacgc agttccgagg
     3301 cattgctcat catttgatcg gctataatcg ccgcgtggac gtagtcagtt tgacaacctg
     3361 cgttttgatc atcgcgcgcc cgctgaaaca aagctgcaaa gcgatcccgg atcgggagaa
     3421 gggcagaagg caggggttcc tcagatgcgg catacagctt cgccattagt tccgctgcaa
     3481 tttcggtcgc ctggtagtcg ccgtgctcgg caacgatgtg agagagcatt cgctccccgg
     3541 catattcgag caacatcaga ttgttctcac gaccgagcaa ccggactgct cccctcccat
     3601 tgcgccatac cagatagtcg gccccgcgca gttcatcagc aatgtcttct ataggtttca
     3661 atcccttgac gattgcagga gtcccgtctg gcaatgaaac tttccaaacg aggctggaaa
     3721 aggtgtccgc aatgagaaca ggttgcgaaa cgtgccaatg agcaggaaaa acaggcggca
     3781 tgaacatcaa ccccaagtca gagggtccaa tcgcagatag aaggcaaggc gttcgcggtc
     3841 gggggcttcg atccccaata cattgaatag gacagcgaag gcgcgctctg cttcatctgg
     3901 cgctgcccag ttctcttcgg cgttagcaat catgagtgcc aaatcggcat agcgatctgc
     3961 tgttccgagc cgcccaaggt cgatcagacc cgtgcattga agagttttag ggtccaccat
     4021 gaagttcggc atgcagggat caccatggca aacaaccata tcggtgcgct cttggtcgag
     4081 ccgcaccggt agctctcgtt cgacacgagc caaaagatcg agctgcggcg tactcttgtc
     4141 ctcgtccggt aagaagtcgg gattgacggc attgcgggac accacatcaa cggcgcgtcc
     4201 gaacattcgc gacagcctgc gctcaaacgg acattgatca accgataggc tgtgaacagc
     4261 gccaagttgc tgccccattg acggccacgc tttgagcaaa tccgctccag acagatcagc
     4321 cgccggtact cccggaattg ccgttatcac caagcatgca ccctcctgtt cctcctgcca
     4381 gttgatgacc tcggggcaag ccacacctcg acctttgagc caaatgaggc ggtcacgctc
     4441 tccagcgagc tcaccgcggc gggaagcagg tgcgattttc gcgaaggcat gcccgtcacc
     4501 acgtcgaaaa acaaaatcac cagattctcc gcctctgaca ggcaaccagt cagaatgcga
     4561 ttcaccaaaa aaaatattag ttcgattcaa tggaggttcc ttcagttttc tgatgaagcg
     4621 cgaatataga gaaatatccc gaatgtgcag ttaacgaatt cttgcggttt ctttcagcgc
     4681 cgccaatacc gccagcccgt cgcgcaaggg gcgcggctcg tgtgtgcgga tgaagtcagc
     4741 tccacctgcg gcggcggcaa gctctgcagc gagtgtcgcg gccccgacat cccccggacc
     4801 acggcctgtg agcgcgcgca gaaaggattt gcgcgaaaca gacagaagca ccggcaaatc
     4861 gaagcgcagc cgcaattcat cgaaccgcgc cagcaccgag agcgaggttt cgggagcagc
     4921 ccccagaaaa aaccccatgc cgggatcaag gacaaggcgg ttgcgtttga taccggcacc
     4981 cgtcagcgcc gcgatgcgcg cgtcaaagaa cgccgcaatg tgatccatga tgtcgccagc
     5041 gggtgcctcg cgccgatctg cctgcccgtc ttgcaccgaa tgcataacga cgagtttggc
     5101 agatgatttc gccaattgcg gatagaacgc agcgtctgga aaaccgcgaa tatcattgag
     5161 ataggccaca ccacgcgaca aggcataggc ttgcgtcgcg ggttgataac tgtcgagcga
     5221 gacgggaatg ccatctgcct tgagcgcgtc cagcaccggc gcgatacgcg cgatttctgt
     5281 gtcggacgaa acaggcgcgg cgtcgggatt gctggatgcc ggaccgaggt cgatcacatc
     5341 tgccccctcg gccatcagct tacgcgcctg cgcaatggct gcgtctggcg ccagataccg
     5401 gcctccatcg gagaaactgt ccgaggttat gttgacgatg ccgaaaatga tgagcgattt
     5461 attcatgggg gcttctataa taataataat cgagcatgag tctcatacgg atgctcgggt
     5521 cgaaagggaa tccccaggcg agtaacctgt ttgcggtgct ccattagctg caggagcaga
     5581 atagcataca tctggaagca aagccaggaa agcggcctat ggagctgtgc ggcagcgctc
     5641 agtaggcaat ttttcaaaat attgttaagc cttttctgag catggtattt ttcatggtat
     5701 taccaattag caggaaaata agccattgaa tataaaagat aaaaatgtct tgtttacaat
     5761 agagtgggta tcggagacga agaaggacaa ggtacatgct gaccgatacc aagctgcgca
     5821 atctcaagcc cagggacaaa ctctacaaag tgaatgaccg ggaaggtctc tatgtggggg
     5881 tcgcctcaga aaacggaatc tatggtcact cccgtttttg caacaccgat tttgacgaca
     5941 agttggcttg cttgaatcta tccggcgtct gaatgggatt ttattcccgc gcctcgatga
     6001 gttccgcgcc tgatgaacct ccagaaaata tacggcttca atgagccttt ccgttttaca
     6061 ggttcctcaa caggccggtg ggccgttagt atcatcaata tcagtattcg caaaaccaga
     6121 tgaatgattg tttaaactgg tgtatttctg cctttatgct tcgtaagttt gctgtcgcgc
     6181 cgtcagtgcc caggctattc tggccagctt gtttgccaga gcacaggtga cgacaaagtt
     6241 gcttttccga cacaacaact ccctgaccca gtcggccaac ttgccagact ggtgttccag
     6301 tttttgtatg aataccctgg cacactgaac caacaaagtt cggatctttt tgttgccccg
     6361 cttgctaatc cctaacaatg tcgtccgacc tcccgtgctg tactgtcggg gtaccagccc
     6421 tgttgccgcc gcaaagtcac ggctgctggc gtactgcttc ccgtcgccaa tctcagttga
     6481 aatagtactg gcagtcagcg ttccaacgca gggaatactc agcaagcgct gtccaacctc
     6541 atcttcgtcc aactttcgtt tcaactgaga ttccagatct ttaatctgct caacaagata
     6601 gtgataatgc tgttgtaatt tcagcagtaa ctggctgaga taaagaggca aactactgtc
     6661 ctcaagaagg gtactcagtc gactaataac ggcagcacct cgcggaacgc tgataccaaa
     6721 ttccagcaga aaagcatgca tctgattagt tgttttcacc ttatcctgaa ccagggattc
     6781 acggacacga tgcagagctc gcattgcctg ctgagattcg gttctgggct gcacgaaacg
     6841 catagatgga cgtgatgctg cttcacagat agcttcagca tcaacgaagt catttttgtt
     6901 gcttttaacg aatgggcgga caaattgcgg tgatatcagc tttggaaaat gccctaactc
     6961 ttccagcttg cgtgccataa agtgagaacc gccacaggct tccatcgcga tggttgttgc
     7021 cgggcatgtc gccagaaatt cgattagctt tggtcgggtg aattttttac ggtaaacggc
     7081 cttcccacga tgatcctgac aatgaatatg gaaagagttc ttacccagat cgataccaat
     7141 aagcgcaatg ttttccatga tggttctccg aatgaaagcc tgtcctcagc atagtactgg
     7201 gaaggaggga gtgaccatct cattaaataa agcacgctaa gccggttgca gcggtcgtag
     7261 cggcctgaac ttgcccgcgc cgatcttggc gctgctgcgc cagaggtaat cgccggtgag
     7321 gttgatgtgc tcccaaccga gcggcgacag gtactgcaac agcgcgtcat caacggcatg
     7381 gccgttgcca cgcagcgcgt gcgcagcccg ttccagatag accgtgttcc acaacacgac
     7441 ggcagccgtt accagattga ggccgctagc ccggtagcgc tgctgctcga aactgcggtc
     7501 gcggatttca cccaggcggt tgaaaaacac tgccctggcc agcgcattgc gcgcctcgcc
     7561 cttgttcagg ccggcatgca cgcggcggcg cagttccacg ctttgcagcc agtccaggat
     7621 gaacagcgtg cgctcgatgc ggcccagctc gcggagcgcc acggccaggc cgttctggcg
     7681 tgggtagctg ccgagctttc ggagcatcag ggaggccgtc accgtgccct gcttgatcga
     7741 ggtggccagc cgcaggattt cgtcccaatg ggcgcggacg tgcttgatgt tgagcgtgcc
     7801 gccgatcatg ggtttcagcg cgtcataggc ggcgtcgccc ttcgggatgt agagcttggt
     7861 gtcgcccagg tcgcggatgc gcggcgcgaa gcggaagccc aggaggtgca tcagggcgaa
     7921 gacgtgatcg gtgaagcccg ccgtgtcggt gtaatgctcc tcgatccgca agtcggactc
     7981 gtggtacagc aggccgtcga gcacgtaggt cgaatcgcgc acgccgacgt tcacgacctt
     8041 ggtgtgaaat ggcgcgtact ggtcagaaat gtgggtgtag aacgtccgcc ctgggctgct
     8101 cccgtatttc gggttgatgt ggccggtgct ctcggccttg ctgccggtgc ggaagttctg
     8161 gccgtccgac gatgaggtgg tgccgtcgcc ccagtgctcg gcgaagggat ggcggaactg
     8221 tgcgttgacc agatcggcca gcgccgcccc gtaggtttcg tcgcggatgt gccaggcttg
     8281 cagccaagcc agcttggcgt aggtcgtgcc ggggcaagac tccgccatct tggtcaggcc
     8341 caggttgatc gcgtcggcga ggatcgtggt cagcaacagg ttcttgtctt tggccgggtc
     8401 gcccgatttc agatgcgcga aatgccgagt gaagcccgtc cattcgtcca cctccagcag
     8461 cagttcggtg atcttgacgt gcggcaggat cattgccgtc tggtcgatca gcgcttgggc
     8521 ggtgtcgggt accgccgcgt cgagcggcgt gatcttcaag cctgactcgg tgatgatggc
     8581 gtccggcagc tcgttggccg tcgccatacg gttgacggtg gcaagctgtg tttccagcag
     8641 cgtcagccgg tcgttcaggt accggttgca gtcggtggcc acggccagcg gcaattcgct
     8701 ggcctgcttg aggctggcga atttcgcggg cggcaccagg tagtcctcga agtccttgaa
     8761 ctggcgcgac ccctgcaccc agatgtcgcc ggaacgcaac gcgttcttca tctccgacag
     8821 cgcgcacagt tcgtagtagc gccggtcgat gccggtgtcg gtcatgac
//
LOCUS       DADLSB010000157         8840 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.157, whole genome shotgun sequence.
ACCESSION   DADLSB010000157 DADLSB010000000
VERSION     DADLSB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.157"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(160..1131)
                     /locus_tag="JN331_003398"
     CDS             complement(160..1131)
                     /locus_tag="JN331_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HAY7261453.1"
                     /translation="MKRAPVIPRHTTHSQSTEDTSSPAPAAPMVDSLIARVGAMARGN
                     AISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSDVIEIADGSRRRKAAILTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSA
                     RSGEALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSPASRVNLS
                     SRHQFAPGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKPGV"
     gene            complement(1131..2297)
                     /gene="sopA"
                     /locus_tag="JN331_003399"
     CDS             complement(1131..2297)
                     /gene="sopA"
                     /locus_tag="JN331_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HAY7261454.1"
                     /translation="MGLMDTLNQCISAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DAFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPA
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3038..4048
                     /locus_tag="JN331_003400"
     CDS             3038..4048
                     /locus_tag="JN331_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7261455.1"
                     /translation="MTSENNSLLLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLF
                     VPTLKSTGKSKANRKNVTDATEELVQLAIAKSEGYTDVKITGSRLDMDTDFKVWLGII
                     RSMSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLKFQSETK
                     GWTTHLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYT
                     YIESLPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRAT
                     YFSVHYRNPKLISSPVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPLKLAEALSAMKP
                     EN"
     gene            complement(4854..5183)
                     /locus_tag="JN331_003401"
                     /pseudo
     CDS             complement(4854..5183)
                     /locus_tag="JN331_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5301..6026
                     /locus_tag="JN331_003402"
     CDS             5301..6026
                     /locus_tag="JN331_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7261456.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLEALTRTGQGHRIDILPTPPATLAPEIFPDRHWLLVTRGK
                     LTAARKKQIEQWQQQVSLEVIIL"
     gene            6126..6536
                     /gene="uraH"
                     /locus_tag="JN331_003403"
     CDS             6126..6536
                     /gene="uraH"
                     /locus_tag="JN331_003403"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7261457.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            6639..7598
                     /locus_tag="JN331_003404"
     CDS             6639..7598
                     /locus_tag="JN331_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HAY7261458.1"
                     /translation="MTTKPVLGISGCLTGSAVRFDGGHKRMGFVMDELAQWVSFRPVC
                     PEMAIGLPTPRPAIRLTLTDSGETQLRFSKPPHDDITQKMADFTADYLPKIGDLSGFI
                     VCAKSPSCGMERVRLYDENGNRGRKEGVGLFTAALLETYPWLPVEEDGRLHDPVLREN
                     FIERVFALHELNTLRAKGLTRRALLDFHSRYKLQLLAHHQAGYREIGPFVASLHEWED
                     LDAFFVAYREKLMTILKKPASRKNHTNVLMHIQGYFRNQLNSRQRVELRDVILHYRDG
                     LLPILAPLTLLKHYMAEYPDRYLMTQNYFDPYPDDLGLRLAVT"
     gene            7744..8832
                     /locus_tag="JN331_003405"
     CDS             7744..8832
                     /locus_tag="JN331_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7261459.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMKRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALASLIAEASRYSQIWLTSHSPEL
                     ANLIEKQRSFSLYQLSMVEGETKVERLG"
BASE COUNT         2234 a         2335 c         2256 g         2015 t
ORIGIN      
        1 ggcgatgcag gtgggaccgt ggtcccaggg agataacctg agcggcgata caggtgggac
       61 cgtggtccca gagagaatat tcaggccagt tatgttttct ggcctgtgag aaaggaaatg
      121 aagtaaaggg agatcaccgt agactaaagc gtgtccgcat cagactcctg gtttctcaag
      181 ctccttaaga atggcttcta ttttctctat acactccgca ggaatgcggg acctatccag
      241 gttaagcacc attttatcgc ccttatacaa tgctgttgcc cctggagcaa actgatgacg
      301 tgagctcaga ttaactcttg atgcgggaga ttgtttcagt acggatgtta aaagactaat
      361 gacctcttca gcttcaaaga ttagtcccgc tttcttctga tcgtggagag tctcagcctg
      421 ctgcttcagt aattcttctt tatctgcaaa agccttctga agggcttcac ctgaccgggc
      481 agacagttct ccaggatgtg caaacagggc aacaacggat ttaggcaatt tggccgtatt
      541 gatacagcgg gtaatgatct tacgagagat gttttccgca tcggcaagcg cagaaatatt
      601 tcctgcaaac tcattctgca gccggcttgt ataacgcagg ccacgttcat aagcactcgt
      661 cggccgataa tcgttaccca gtcgggacaa tgcagccatc tgctcatcgt ccagttcacc
      721 aaccagaacg cgataatcac tttccgtcag gattgcggct ttacggcgac ggctgccgtc
      781 ggcaatttct atgacatcag aaacccggcg gccgaaagct ggagtctgct gaccggtcag
      841 taaaaatgaa ggaatgagat catccagagc atcttcggta agtagttcct gatcgcgctc
      901 attgcctgac catacgcgtg aagcgctctc aacagagtct ccccggagca cctcaagggt
      961 aaatttcact tcccgtccac ataccggaag agagattgca ttaccgcggg ccatagcacc
     1021 cacgcgcgca attaatgaat ccaccatcgg cgcggccggc gccggtgatg aagtatcttc
     1081 agtcgattga ctatgtgtgg tatgtcttgg aatgacagga gcacgcttca ttatctaatc
     1141 tcccagcgtg gtttaatcag acgatcaaaa atctcattgc aaacaggctc ccagatagag
     1201 agggcattac gccaggcacc ggttgaagaa cgttgatcaa tagcctgttc aaaaacggtt
     1261 ctcatacgga tctgaccttt accgacttca tccgtttcgc gcacaacatt tttaaggacc
     1321 atgcttcccc aggcatcccg aatttgttct tccatccacg gggactgtga gccattatta
     1381 ttactgtatt tggtaagcag aatacggaca tctggctcga agcctttaag atccacgttc
     1441 ttaagcaggt cacgaagcat atcgaaaaac tgcagtgcgg aggtgtagtc gaacagctct
     1501 gccggagtag ggacaatcag aacatcagca gcgcatacaa catttatcgt accgatacca
     1561 aggttgggtg cactgtcgat aactatcacg tcataatcat gagcgacagt ttcaatggcc
     1621 agacggagca tcaggtgcgg gtcagcaggt aatttgcctt cgtcaaattt ccccatcagc
     1681 tcggtttcaa tgcggtgcag tgccagacag gacgggatga tatcaaggcc aggccagcag
     1741 gtgggcttta tagcgtagct ggcgtcatcc ttttccccaa gataaaaggg caggagagtg
     1801 tcctcagcat gaatatgaag atcgggcacc catccgtgat acatagaagc agttccctgt
     1861 gggtcattgc cttcaacaag cagaacacgg agtcctttca gagcaagatc ctgagccagg
     1921 tgaacggata ctgaggtttt gtaaacacca cctttgtgag ctgcaacgcc aatcaccggc
     1981 ggaaacgcat cttcagcacg gcgcagtcgt gtaccaaata catcccgcat gtgattgatt
     2041 tgttcgatgg tataaccaac gcgttgctca actctcccac gggtttccat atcagggtgc
     2101 ggcagccggc cagctttctc ggcatccctg atagcctgag atgacacgcc aactaaatct
     2161 gccgcttcac caatcctcca gcgccgggtt attttccggg cttccggact gtcatcatta
     2221 aactgtgcaa tggcgattgc tttcgtcatt tcatgaccag cacttatgca ctggttgagt
     2281 gtatccatga gtcccatact gaacatcctt taatcatcac tttgcattat tttattaaat
     2341 cttggaattt attgcaaagc aacaataaaa tcgcaaagca cacgaaaaag tgcaaagtga
     2401 accacattgg gtaatgaaag aacgcagggg attacaaaag agtacatacc gcagtcaata
     2461 ttaatcactg ccgctcgccg cagtaagcga tctcacacag tgagaaagcg gcgaggaagc
     2521 ggaaaatcac agaactcaca gattgcacgt acgtcgggtt gctaaagatg actcgggatc
     2581 agccagttat aatgaaattg actgcggtat gtaatatatt gactatggta tgatgcttac
     2641 cgcatctgta ggaaagacga ctcagcatac cgcagtcaat gtttcattgt tgtacctgta
     2701 gatctcaatc ttccagagcg tattcaggaa aatcaggcca ggggtctgga tatctggtgt
     2761 ggcgcttccg gccttgtatc tgggttattc atggtctcga tccaataatt gatcccaagg
     2821 agatcatagt gagatccaaa agagatcccc ggatcgctcc agcatgcgct gtaactggct
     2881 ttaacgtctg tctggatcga ctgcggaatg taaaatgttg actgttatat gcataatcta
     2941 atactgtgat atgcaacggt atttactgag gtatgtatat ccgttgactg cgatacgtat
     3001 tactacatcc ttttgtggat aaccgttgag gatcaagatg acgagcgaaa ataatagctt
     3061 acttctgaac cttcaggaag ttgataagac aaccggtgaa gttgttaagc tggatgtcaa
     3121 cagcaccagt accgtgcagc cagtagcgct catgagactc ggtctcttcg tcccaactct
     3181 gaaatcaaca gggaagagca aggcgaaccg gaaaaacgtt acagacgcga ctgaggagct
     3241 tgtacagctg gccattgcca aaagcgaagg atacactgac gttaagatca ccggttcgcg
     3301 tcttgatatg gacacggatt ttaaagtctg gctggggata attcgctcca tgtcggagta
     3361 tggggtaaaa agtgacaccc tggaactgtc gttcgtcgaa ttcgttaaga tgtgcggatt
     3421 tgactcccgt cgttcaaaca aaaaaatgcg cgatcgcatc agtaattccc tgtttaaact
     3481 cgcctcggtt acgctgaagt tccagagcga gaccaaagga tggaccaccc atctggtgca
     3541 gtcagcctat tatgacatca acgaggatat cgttgagatc aaggctgaac ctaagctgtt
     3601 tgagctttac cacatggaca gaagggtact gctgcggctg aaagccattg acgccctgca
     3661 gcgcaaggaa tcagcccagg cgctttatac ctacatcgaa agtcttcccc agaatcctgc
     3721 acctatttcc atgaaacgga tgcgtgaccg gctgaatctc acctcaaatg tctatacaca
     3781 gaaccacacg gtgcgtaagg cgatggagca gctgcgtgat attggttatc tggattacac
     3841 cgagtttaaa cgtggccgag ccacctattt cagcgtgcat tatcgcaacc ccaaactcat
     3901 cagtagtcct gtgaaggttc cgcgcaaaga ggaagaagag aaggcgccag agcaaaatta
     3961 tgacgaagtc attaaggcgc tgaaggctgc tggcatcgat cctcttaaac tggctgaagc
     4021 cctctcagcg atgaagccag aaaattgact gcgatatgca tatcatgact gccgtatgcc
     4081 gctaaggaca ttccattgac tgtgatatgc atcacgcata ccgcagtcaa tatttattct
     4141 ctatcatgtc tgcccggatc gaacaatatt gactgtcata tgtgatttcg ggtcatgata
     4201 cgcagatatt gactacggaa tgcgggttta atgggggtaa tccctggtct gttcaccatt
     4261 ccatggggga taacatgcca cagtcaattt ttcagtaacg ctctatatcc cttacgaaaa
     4321 tctcaatcgg gtgctgagac ggtcatgtta cctgacggta tccgttcccc tgtgtactgc
     4381 aggtttagcc cggcaggatg agactgcagc tgtctgtata acccggcgcc agcttcacac
     4441 tgttaaatgc gatcaggaaa cgggagccga atggcagaaa ggtgttggct agggaaggtg
     4501 cgaataagca ggttatttct tcccaagctg actcgctgat taaaatttcg cggatctggg
     4561 ccgatttttt tcccgcaaac acatcgaatc agcctattta ggggtcagtt tggagatcgg
     4621 aaatttttgt acgttaagca cattatttaa ccaattcgga tacgtaagtg attgatttat
     4681 cgtcattggc tatcgctttt aggttgtcgc tcttgctgta tcgcatttag gttatccgat
     4741 taagacaaaa tcccctgaaa acgctgtata gcgcgggagt acaccctatt gctacaggta
     4801 agcctgttca aaaaacaggc ttaccgtaca ataattctct atatccaaac tgaccccaag
     4861 aaggacacct tcagcgcggc cgtggtcggt tcgccggtaa agtaggcttt accctcgctc
     4921 tcattccact tgttcttcac agggtcgtag ccgcggttaa gcacgctgat gccgccgtcg
     4981 ctgcgcttgc cgtaggtggc ggtaacctgc tctaggccgc gttcaaagcg gttttccagg
     5041 cgagcgactt cgtaccattt gccaagatag cggctggcgt caaaaccgga aatgggctgc
     5101 acgcccttcg gtggggtagg ggatttacag gctaccagcg tcagggcaat ggcaacaccg
     5161 gtcacaacag gccatagctt catgagaatt cctttcattt gcacggttgg aaattaagtg
     5221 tagtgcaccg cggcggcgct tcatcccatc cgcgaaaatt cgtagtatat tgagagtatt
     5281 cctcaactct ggacacggac atggcttact ccatcggcga attcgccaga ctcagcggga
     5341 tcaccgccac cacgctgcgg gcctggcagc ggcgctacgg cttactcaag cctcagcgca
     5401 cggagggcgg ccaccgccag tacagcgatg aggacgtcca gcaggcgcta aaaatcctcg
     5461 actgggtaaa aaaaggcgtc ccgattggcc aggtcaaacc gctgctggag cgccctacgt
     5521 cgcgcctgac caacaactgg ctcacgctgc agcagagcat gttacagcgc ctgcaggacg
     5581 gcaaaatcga gtccctgcgt cagatgattt acgacgcagg acgcgagtac ccaagaccag
     5641 aactggtcgc gaacgtgctg cgtccgctgc gcagtcaggt gtcggcaaac gtcgccgccg
     5701 ccatgaccct gcgggagatc ctcgacggca tcatcatcgc ctacacctcg ttttgcttgg
     5761 aaggcgataa aaaagcgccg ggcgacaaca tcctgctcag cggctggaac ctgaatgacc
     5821 cgtgcgaagt ctggctcgaa gccttaaccc gcaccgggca aggccaccgt atcgatattt
     5881 tacccacgcc gcccgccacg ctggccccgg agattttccc ggaccgccac tggcttctgg
     5941 tcacgcgcgg taaactcacc gccgcgcgca aaaagcagat cgagcagtgg cagcagcagg
     6001 tatcgcttga agtgattatc ctctaatcat tttctcctgc gggaatcttc acgaaaagtt
     6061 gaatattttc ccgcgccaca aatgctaacg ttttcctgtg accgacaacc ccacaggaaa
     6121 acacaatgaa aaaaactgcc tctctgctga tgctgaccac cctggccttt gcccctgccg
     6181 cctttagcgc ccctgccggc acgctgagcg tacacattct tgaccagcaa accgggatgc
     6241 cgccatcgga cgttaccgtc acgctggaaa aacagcagca ggacaaatgg accccaattg
     6301 ccagcggcaa aactgaccat gacggccgca tcaaatcgct ttatccggag gatcaggata
     6361 tgcagcccgg cgtctataaa gtggccttta aaaccgcaga ttacttccac gggaagaagc
     6421 tggactcctt cttcccggag atcccggtgc tgttcaccgt aacccggacc aacgaaaagc
     6481 tgcacatccc tctcctgctg agccaatacg gttattcaac ttacaagggc agttaagaaa
     6541 cgttgatttt aaaggcaaat aaaaaataat tccgctaata cttggacaaa ttagttatgt
     6601 tgtgtaagtt ttaaagagaa gaacgggagg accacaccat gacaacaaaa cctgtgctcg
     6661 gtattagcgg atgtttgacc gggtctgccg ttcgcttcga cggcggacac aagcgtatgg
     6721 gctttgtcat ggatgaactg gcccagtggg tgagtttcag gcccgtctgc ccggaaatgg
     6781 cgattggcct gccgacgcca cgcccggcca tacgcctgac gctgacggac agcggcgaaa
     6841 cgcagctccg cttcagcaag ccgccccatg atgatataac gcagaaaatg gccgacttta
     6901 cggcggacta tctgccaaaa atcggcgatc tgtcgggatt tatcgtctgc gcgaaatcgc
     6961 caagctgcgg catggaacgc gtgcggctat atgatgaaaa cggcaaccgg ggccgcaagg
     7021 agggtgtcgg gctgttcacc gccgccctgc ttgaaaccta cccgtggctg cccgtggagg
     7081 aagacggtcg gctgcacgac ccggtgctgc gggaaaactt tatcgagcgg gtatttgccc
     7141 tgcacgagct gaacacgctg cgcgctaaag gcctgacgcg ccgcgcgctg ctggatttcc
     7201 acagccgcta taaattgcag cttctggcgc accatcaggc gggctaccgc gagattggcc
     7261 cgttcgttgc gtcgctgcat gaatgggaag atctggacgc gttcttcgtg gcgtaccgcg
     7321 aaaagctgat gaccatcctg aaaaagcccg cctcgcggaa aaaccatacc aacgtgctaa
     7381 tgcatatcca gggctatttc cgtaaccagc tgaacagccg ccagcgcgtc gagctgcgcg
     7441 atgtgatcct gcactatcgc gacgggctgc tacccatcct cgcgccgttg acgctactga
     7501 aacactacat ggccgaatac cctgaccggt atctgatgac gcaaaactac tttgatccct
     7561 atcctgatga tttgggtctg cgtctggcag taacctgacg ataaaaatgc gaaggcccgt
     7621 ttgacccaca gaggcccaaa gcagtacccc gtttagacga caccctcact gtcgtctctt
     7681 ttttgcattc cacacaaaag actgtaatta tgtacaggat tgtaacgcaa aaagggactg
     7741 cctgtgatca ccaccctgca cattcaaaac taccgctcga ttcgcgaaat gtcgctggag
     7801 ctggaacagc tcaacatcgt ctttggccca aacggcaccg gaaaatccaa tatctacaag
     7861 gcgatccacc tgatgcacag cgccgcacag gggcagtttt cgcaggcact ggcgaacgag
     7921 ggtgggattt taaaagtgtt ctgggcaggc aaaacccgca gcgaccagct gcggcggatg
     7981 aatttggccg ttgaaaccga aacctacgaa tacgagctgc aggtaggctt tgtcgaaaaa
     8041 ctgccttacc cctcacagtt tcaactcgat ccggtgatca aagaagagtc catctggctg
     8101 agtggtcaat accgccgccc gtcgtcacag ctgatgaagc gtaaaaatca ggccgtattt
     8161 ctgaacaacg tgcatcacga gaaagtcacc cacagcggca cgctgtatga gaacgaatcg
     8221 gtgttcggcc agcttggcga gccgcatctt tacccggaag tgtcgcagat gcgcgagtcg
     8281 ctgcgtaact ggcgctttta tcaggagttt tctgtatcaa tcggctcggc catgcgcgcg
     8341 cctcaggtcg gtttccgctc cccagtgctg gccagcgacg gcgcaaacct ggcggcggcg
     8401 tttcagacca tcgtggaaat tggcgacgag ctgctgctga tgcgcatcct cgaccaggcc
     8461 ttccccggct gcgtgtttta cagcgacaac accggcggac gcttccggat gatgatgcag
     8521 cgtgaagggc taagcagacc gctggagccg gcggaattct ccgacggcac tctgcgtttt
     8581 ctgtgcctgg cggtggcgct attaagcccg cgtccaccgg cctttatcgc cctcaacgaa
     8641 ccggaaaaca gcctgcatcc gcagatgctg cccgcgctgg cgagcttaat cgccgaggcc
     8701 agccgctact cgcaaatttg gctgaccagc cactcgccgg aactggcgaa tttaattgag
     8761 aaacagagat cgttttcgct gtatcagctg tcgatggtgg aaggagaaac gaaggtggag
     8821 aggctggggt agtctcccag
//
LOCUS       DADLSB010000158         8771 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.158, whole genome shotgun sequence.
ACCESSION   DADLSB010000158 DADLSB010000000
VERSION     DADLSB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.158"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(524..892)
                     /locus_tag="JN331_003406"
                     /pseudo
     CDS             complement(524..892)
                     /locus_tag="JN331_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1418..2392
                     /gene="hemB"
                     /locus_tag="JN331_003407"
     CDS             1418..2392
                     /gene="hemB"
                     /locus_tag="JN331_003407"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY7261460.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(2498..3349)
                     /gene="tauD"
                     /locus_tag="JN331_003408"
     CDS             complement(2498..3349)
                     /gene="tauD"
                     /locus_tag="JN331_003408"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAY7261461.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3346..4173)
                     /gene="tauC"
                     /locus_tag="JN331_003409"
     CDS             complement(3346..4173)
                     /gene="tauC"
                     /locus_tag="JN331_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY7261462.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(4170..4937)
                     /gene="tauB"
                     /locus_tag="JN331_003410"
     CDS             complement(4170..4937)
                     /gene="tauB"
                     /locus_tag="JN331_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY7261463.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLLLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4950..5876)
                     /gene="tauA"
                     /locus_tag="JN331_003411"
     CDS             complement(4950..5876)
                     /gene="tauA"
                     /locus_tag="JN331_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7261464.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQVDNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSGID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(6144..6659)
                     /locus_tag="JN331_003412"
     CDS             complement(6144..6659)
                     /locus_tag="JN331_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261465.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARAWGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            6853..7104
                     /locus_tag="JN331_003413"
     CDS             6853..7104
                     /locus_tag="JN331_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7261466.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            7106..8771
                     /locus_tag="JN331_003414"
                     /pseudo
     CDS             7106..8771
                     /locus_tag="JN331_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2206 a         2280 c         2173 g         2112 t
ORIGIN      
        1 tctgcatagc attatcagca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc acaatcagtt gtcatgcact
      541 cggaataaat cataccgtga atagtgctgt ttttgattgt cagactattg gcgttggtat
      601 tggtagaaat accgtcgtcc agatagtagg tagaaatcac gccgttaacg gtagcgttat
      661 taatgaccgg gtagatatcg ccgttgtaat agctatcagc ggcatagttg ttccagccta
      721 cataaccatc atagtaaacg tcatcagcat aggtggcgtc attgtagtga tggaaagtat
      781 tgtaagtttg accagaaata tcgttggtcg ctgcattagc ctgagaggtg atagccagag
      841 tgcaagccaa tgctaattgt gatactacaa gtttcttttt ccaggagtgc atttgtcatc
      901 cctcctcagg gacgtaataa gttgatccat caattatcaa tgcatagaaa acatattatg
      961 tttcgcgcta acgattatgc tgcgttacag acgaatggtt caattattct ttcccctaag
     1021 tccgaatgtg gacatatata atttatggag aaaaacacgc atcaaattga taaaaggacg
     1081 atatttcagc atgttgcaga taatgttatt tttgatacat acgcgttata aatatcaaaa
     1141 acacaaacca gataaatatc ttatcaatat acaacaaagt ttgaatcatt caatttatag
     1201 cagcacaacc aaacgccaaa aaaaataata accaagagct ataaataaag attaaaaata
     1261 ttttcagtga atatgaaatt attgctattt ctattacaaa gatgtcctgg tctctctttc
     1321 attctcttct tgtcaacatc gcgacaactt tcgtaaaaca ctcctgcccc gcttcaggta
     1381 tactgtgcac cttaattcca caaacatcag gcagaccatg acagacttaa tccaacgccc
     1441 tcgtcgcctg cgcaaatctc ctgcgctgcg cgctatgttt gaagagacaa cacttagcct
     1501 taacgacctg gtgttgccga tctttgttga agaagaaatt gacgactaca aagccgttga
     1561 agccatgcca ggcgtgatgc gcattccaga gaaacatctg gcacgcgaaa ttgaacgcat
     1621 cgccaacgcc ggtatccgtt ccgtaatgac ttttggcatc tctcaccata ccgatgaaac
     1681 cggcagcgac gcctggcggg aagatggact ggtggcgcgt atgtcgcgca tctgcaagca
     1741 gactgtgcca gaaatgatcg tcatgtcaga cacttgtttc tgtgaataca cttctcacgg
     1801 tcactgcggt gtgctgtgcg agcatggcgt cgacaacgac gcgactctgg aaaatttagg
     1861 caagcaagcc gtggttgcag ctgctgcagg cgcagacttc atcgcccctt ccgccgcgat
     1921 ggacggccag gtacaggcga ttcgtcaggc gctggacgct gcaggcttta aagatacggc
     1981 gattatgtcg tattcgacca agttcgcctc ttccttttat ggcccgttcc gtgaagctgc
     2041 cggaagcgca ttaaaaggcg accgcaaaag ctatcagatg aacccaatga accgtcgcga
     2101 ggcgattcgt gagtcactgc tggatgaagc ccagggcgca gactgcctga tggttaaacc
     2161 tgctggagcg tacctcgaca tcgtgcgtga gctgcgtgaa cgtactgaat tgccgattgg
     2221 cgcgtatcag gtgagcggtg agtacgcgat gattaagttc gccgcgctgg cgggtgctat
     2281 agatgaagag aaagtcgtgc tcgaaagctt aggttcaatt aagcgtgcgg gtgcggatct
     2341 gattttcagc tactttgcgc tggatttggc tgagaagaag attctgcgtt aatttttttc
     2401 ctctcagaaa gggggagtta acctgttgaa agtacggtga acgccccctc accctaaccc
     2461 tctccccaag ggggcgaggg aaccgtccgc actcgtatca ccccgcccga taaaacggtt
     2521 tatccccaag gatcgtcgcc cgatgcataa tccgccgctg tggcaggtaa tcggcattgg
     2581 catagtgctg agtcacgcgg ttatcccaaa tcgcaatatc attcggttgc cagcgccaac
     2641 gcacctgaaa ctccggttta gtgatatggg cgaataaaaa gcctaacaag gcttcgctct
     2701 ctttctcgct gacatcgaca attcgcgtag taaagccttc attcacaaac agcgcctgtt
     2761 taccgctcac cggatgcgtt cgcaccaccg gatgtagcaa cggaggattt ttcgcgaccg
     2821 cctcgcgcca gcgttgatgt tcctcctcgg ttttgcggta tttgtattcc gggaatgatt
     2881 tacggaaatc atgctccgca cgcagccagc tcagcagctg gcggaaagga acagagagcg
     2941 cctcataggc cgcaataccg ctggtccaga gcgtatcacc gccggtcgaa ggtaactctt
     3001 tagctgccag aatcgcccct gcgggtggcg tttcaataaa tgtcacatcg gtatgccagt
     3061 tgtcgttatc cggcggatta tcgttatggg tatccagcac gatgatctcg tcaacccctt
     3121 cggcatgagg gtaaacaggg tgaatatgca gttcgccaaa acgctgggcc agcgcgcgtt
     3181 gctgctgcgg cgtaatagct tgatcgcgca gaaataccac ctgatggcgc agcaccgcat
     3241 ggtaaagctg ttcaaactga ttatcgctta acgggcgcgt caggtcggca cccgaaattt
     3301 gtgcgccgat atacggcccc agcggggtaa tgctcagacg ttcactcatt gtacttctcc
     3361 atgccagggc gtcagacggc gctgtaacgc gcgcagaccc agttctaaaa gaaaggcgat
     3421 aatcgcaatc accgcgatcc ccgccagtac cacgtcagtc gcgagaaatt cacccgctga
     3481 ctgaaccata aatcctaaac cgcgtgtcgc ggcaatcagc tccgccgcta ccagcgtaga
     3541 ccagcccaca cccagaccaa tgcgtaatcc ggtgaggatt tccggcagcg cgccgggcaa
     3601 aatgacaaac cacagcacct gcgcacggct ggcacccagc gactgggcgg cacgaatgcg
     3661 aacctgctgc gcgcttttca cccctgccag cgccgacatc gccaccggcg caaaaatcgc
     3721 taaatagatc agtaagatct tcgaggtttc accaatacca aaccagatca ccatcagcgg
     3781 caaataagcc agcggcggca ccggacgata aagttcgatt accggatcca gaatgccgcg
     3841 caccgtcggg cttagcccca tggcgatccc gaccggaata ccgatcacca ccgccgcgag
     3901 tagcgccagc acaatgcgcg tcaggctggc tgccagatgc tgccacagcg tggcgtccat
     3961 aaatccctgc ggtccggcaa tggtgagcag ttttgccagt acttgttgcg gcggcggcaa
     4021 aaacagcggg ctaatcagtt gcagcgccgc caccgtccac catacagtga gtaaaaccgc
     4081 taacgtgcca atgcttaagg tcacctgacg cgagagcggc cagcgccatt tcagccgccg
     4141 cgaatgcagt ttttcattaa tgagcacact catgagaacg cctcccgttg ctcaaatacg
     4201 cggcttaaaa catattcgcg catggcgatg aattgtggat cggacttgat gctgcggctc
     4261 gactctcccg caacaaagcg gcgagcaaag ttgagcagca gccgctccag cacacggcca
     4321 gggccggatg aaagcagaac cagttcagtc gccataaaca ccgcttcttc tatatcgtgg
     4381 gtaatcaaca gcacctgctt gcccgtctcc tgccagagtt tcagcagcag agtttgcatc
     4441 tggtcgcggg tgaaggcgtc cagcgcacca aacggttcgt cgagtaataa cagctgggga
     4501 ttcgccgcca gcgcgcgggc aatccccacc cgctgacgtt gaccatcgga aagctgccag
     4561 atatagcgtt tttctgcgcc ttccagcccc acttttttca gcatctggtg cgcgatttcc
     4621 agtcgctgca ttttctctat acctgccaat tgcaggccga acgccacgtt gtcctgtaca
     4681 ttgcgccacg gtagcagccc ttcattctga aaaactacgc cacgctctgc tcccggtccc
     4741 tcaatacgct tacccgccag ttgaatgctg ccatgctgat aaggcacaaa accggcaatc
     4801 agattcagca gggtggtttt accgcagccg gacggcccca gcaccaccag tagctcgccg
     4861 ctttccagcg tcaggttgat atcttccagt gccggtttgc cgccataatc ggcatagaga
     4921 tgagagattt gcagcatctg cgcctccttt tattgcacga agcgcgaggt aacgtactgg
     4981 ctgtaatcat tcgctacagc cgggaccttg ccctgctctt tcaaaaactg cgcggtgtcg
     5041 atgattgctt tgttcaccgg tccggtcagt tctgccgttt gttgctgcgg cgtcagatag
     5101 gtatttccct tcaccagccc cggaacgtca ccttcaggca cgccgcttaa acgcgccagt
     5161 ttgctgatgt tttccggctg tttcagccac gcgtcagggt tagcaatgta cggttgctgt
     5221 gcatcgatgc cgcttttggc gaacgctttc acgacctcag gatgtttctc ggcaaaatct
     5281 ttgcgcacca cccagacgtc cagcgttggc gcaccccact gcccgacctg ttcagaatcg
     5341 gtcagcacct tgccgtcttt ttccagggcg ttaaccgccg gtgcccagac ataagcacca
     5401 tcaatatcac cccgctgcca tgcggcgata atcgcgggcg gctgcaggtt cacaatctcc
     5461 actagccctg gtttaatccc ccagtgtttc agcgccgcca gcaggctgta gtgggtggtg
     5521 gagataaacg gtacggcaat gcgtttacca atcaaatctt cgggtttgct gatagttttc
     5581 tttaccacca gcgcttcgga gttacccagt tttgacgcca gcaagaagac ttcaatcggc
     5641 acctgttggc tggctgcaac cgctaatggg ctggaaccga ggttgccgat ttgcacgtcg
     5701 ccagaagcca gcgcccgcac gatgctggct ccgctgtcaa acttacgcca gtccacgctc
     5761 gccccgctct ctttagcaaa ggtgttgtcg acctgagcca ctttcgccgg ttcggctgag
     5821 gtttgatacg ccacggtgac gttcaccgcc tgtgcctgaa aagcgatgaa tgccagtgcg
     5881 gcaagaagtg tgttatatga ctaggtaatt tattcaagat aaccgggaat gggtaaaaaa
     5941 agacgggatt tgatgggatt caataagtta tgcgccgatt gcattcaaga taaaacgagt
     6001 taaaaatgct caatgcttcg atatcgataa aacattcaag ataggcgcgc cgtgtatttt
     6061 aattctttta agactcttca aaaatgtcat cgtagccttt aaaaacgaat tttaaaggcg
     6121 accctaaagg caggtcaatg aagttaaccc gccttcgcgt ttacactctc tgagagaata
     6181 ctagcttcaa tctcttccaa aatctctctg acttgctcgt cgggcatatc aacgagcatc
     6241 aacgctgcca ggcgtttacg gccctctagt tgcagtagct cttgagtttc cttatctaac
     6301 aagcgtagca cagtctcaac acctttcctt gcgagcatgt ttgtaagggc ttctcgttcc
     6361 tcactggtaa gaaacgacaa catttctgtt agctgatccc cttggcttaa atcactttgt
     6421 gttttttgtg tttttggtgg attcccgtta ccggttttta gccattcaag actaacacct
     6481 tcactttctg caatgcgtac caatatgctt tctcgaggtt ctggtgtatt tcccttttta
     6541 aaatagcttt gtaacgtatt gatatttatg ccccaggctc tggcggcagc acttcttgat
     6601 ggataccgcc taataagata agccatgcgc tccacgatag gttcttttgc ctgctccata
     6661 aaagtacctc taggtatttt tctgtataaa ggttctttcc catctatcta attgaatgga
     6721 aaggcaattt taaaaatcca aataaaatac aaaaaaaata ccttgtattg gtgctttttg
     6781 tgatctgcga ttttatttac tcggatatcc acgcgaatat ccgcgcgggt aattttgtag
     6841 ggtaaaggaa agatgagaca aagagaagat agtggccgcg attggccttc gctgatgatt
     6901 cagcaagcac tcaaagataa tgggtggtct tcctttcggg agctttcact ccatgccggt
     6961 tatgctcagg attcactgaa agcggtagtt cgtacgccaa acaaaaaata cgaacgtgtt
     7021 atttctgacg ctataggtgt accgccagaa atcatctggc ccagccggta tcgtaacgat
     7081 ggatttatca ggaaggcgtc gtgatatgca gtcgtacgtt accgtaaatg acctgctcgg
     7141 tgttcctgga atgccagcca ccgcgaaagg catccgacag gcgttacagc gtttttctcg
     7201 tgctcttggt gacgttagtc gccgtcgagc aggtacaaaa gccattgagt accatatcga
     7261 ttgcctgcct aaggtaaccc aaaaggcact gcgtgaacgc tatgtggaac aactggtggc
     7321 tacagagaac aacgtctccg aagtgaagac cgttaccagg acggcgcgta accctgatgc
     7381 tgttcaggca gttgaagcat atcggggttc tccgcaacta atggaagaac gcctgaatgc
     7441 cctgactgaa aatcagcgcc gggtgtctga agcaagagcg gcgctggcag tggaagtgct
     7501 gaagctggaa agcgccggta acctcgggcg actgaaagcc attaactttc tggttgaaaa
     7561 agcccgtaaa ggtgagttgc cggaacgact gcaacaggct gcgattaacg ctaatgcaaa
     7621 acgtggcact aatcgcacca tcagccgtga tccgctttac cagtgggtac tgaaatacaa
     7681 ccggtcgcag aacgccgcag aacgactgtt gttactggca ccaggaaaac gcgacgaaat
     7741 aaaaccggaa gaaattagct ggctgcctga atttctggcg cagtatcgtc aggctaacgg
     7801 cagacctatg tcggaagcct acgaggattt tgtcacggaa tggcaacgtc gctatgcgga
     7861 cgaaccgtac atgctggagg tgatgccttc ctatgatgtt gtccggtatg ccatgaaaaa
     7921 attgccggaa gtggtgaaac aaaaaggacg cgttaccggc agtgaatacc gccagcttga
     7981 gggattcacc cgccgcgact ggacagctat gccggtgaat tatgtctgga ttggtgacgg
     8041 ccacggtatg aagctgaaat gtgcgcatcc gatccacggg cgtccatttt caccggaagt
     8101 cacgtttgtt atcgacggtg gaacccggtt tgttgttggc tggagtcttg atcttgcgga
     8161 gaacgtgttt gctgttgccg gggcgataca gcacggaatt cgcaatcacg gaaaaccctt
     8221 cctgtattac tcggataatg gttccggtga aacggcagat atgctggata agagatcgtc
     8281 gggattctgc cccgtctggg tattaaccac cccaccggta ttgccggaaa tccgcaggga
     8341 cgcggcatca ttgaacgtct gaaccggacg ctgccgatgc gtatagccag acggtatcgc
     8401 acctattttg gcaagggggc agaccgggaa acactgcgtg tactcaatcg cgatctgcgt
     8461 tcagcattta atgccctgca acaggacaaa ccgctgaatg cccgccagaa atctgcaatg
     8521 cgcgagctgc cgtcctgatc agaactgatt gaggctattc gtgaaggagt ggaatggtac
     8581 aacaaccgcc cacattctga actgccgatg aaaccaaacg ggaagcatta cagcccggct
     8641 gatttcagaa aaaaacgcct ggcggaagaa aacaccgaaa ttgaatggct gagtgatctc
     8701 gaactgcgcg acatgttctg cccgatggtg gagcgtccgg taagacgctg tgaaatcaaa
     8761 tggcttggga a
//
LOCUS       DADLSB010000159         8553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.159, whole genome shotgun sequence.
ACCESSION   DADLSB010000159 DADLSB010000000
VERSION     DADLSB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.159"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..647)
                     /gene="yihS"
                     /locus_tag="JN331_003415"
     CDS             complement(<1..647)
                     /gene="yihS"
                     /locus_tag="JN331_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY7261467.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRV"
     gene            complement(664..1542)
                     /gene="yihT"
                     /locus_tag="JN331_003416"
     CDS             complement(664..1542)
                     /gene="yihT"
                     /locus_tag="JN331_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HAY7261468.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(1566..2462)
                     /gene="yihU"
                     /locus_tag="JN331_003417"
     CDS             complement(1566..2462)
                     /gene="yihU"
                     /locus_tag="JN331_003417"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY7261469.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVARTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            2630..3526
                     /gene="yihV"
                     /locus_tag="JN331_003418"
     CDS             2630..3526
                     /gene="yihV"
                     /locus_tag="JN331_003418"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY7261470.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITSQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            3560..4345
                     /locus_tag="JN331_003419"
     CDS             3560..4345
                     /locus_tag="JN331_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7261471.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            4444..5043
                     /gene="yihX"
                     /locus_tag="JN331_003420"
     CDS             4444..5043
                     /gene="yihX"
                     /locus_tag="JN331_003420"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY7261472.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            5037..5909
                     /locus_tag="JN331_003421"
     CDS             5037..5909
                     /locus_tag="JN331_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY7261473.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            5906..6343
                     /gene="dtd"
                     /locus_tag="JN331_003422"
     CDS             5906..6343
                     /gene="dtd"
                     /locus_tag="JN331_003422"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY7261474.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            6388..7329
                     /gene="fabY"
                     /locus_tag="JN331_003423"
                     /gene_synonym="yiiD"
     CDS             6388..7329
                     /gene="fabY"
                     /locus_tag="JN331_003423"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY7261475.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            7745..8553
                     /locus_tag="JN331_003424"
                     /pseudo
     CDS             7745..8553
                     /locus_tag="JN331_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2089 a         2071 c         2240 g         2153 t
ORIGIN      
        1 acgcgataat gattatttct ggcgacgtcg tggataatca cggaagccac gcgaatcgcg
       61 cgatccagcc attttttgtc gtgagtgacg tcataaacaa tcaagaaagc ttccaccgcg
      121 tgcatattgg cattgccgcc gcggtactct tcggttttgc tgaaggcttc gtcccaggat
      181 tccaggcaca tctgctcttc ttcgctccag aaatatttct cgataatctc aatggtgtaa
      241 tcgagcagct tgcgcgcttc cgggtgaccc gttgtgacgg cgctggcggc acccagcaga
      301 gcaaagaagt gttgatagcc ctgtttggag gcatccacca cgccctcgtc attcacgcag
      361 gcataccagc cgccatattt tttatcacgc agtgcgccgt tcatggcttt aataccgtga
      421 tcaaccaacg cgtaagcgcc aggtcgaccc atcgccgcag caacggaata aacgtgcaac
      481 atacgagctg tgatccacag atgggtgccc atctcttctt tgatttgccc tttattgcct
      541 aaccagccaa aaccagtcgg cactacggaa tttttaccaa aatcaaagat gcggtcggtt
      601 tcctgttcca gccaacggtt gtggcttagg gtgttaaacc atttcattcc ttttatcctc
      661 atcttagcgg cgtttagcca tcatttcgtc gacgatatcg ccaagttgtt gtaatttcgg
      721 tgcgcaaacg tcacggagca tcagctcgtt gtctggtaaa ccgacgaccg atgcccagac
      781 tgcacggcct gccaggaatc ccgatgcgcc cgccgtcatt gccacgcgta cggcacgcgg
      841 gaacagtttt tcgtcgacac cggaagagag gatcacccat ggcatattga tatggtcatt
      901 cagacgttgt gaagcacaga gaagctcttg ttgcggacct ttgccataaa gcggcatttc
      961 aactttgtag aggtcagcgc cactgtcgcc cagctctttg gcggcatcga tgatcgcttg
     1021 ttcgcgatcg aatttatcgc cacgacgcgg tgggcggacg actggctcaa tgatgcttac
     1081 cagaccgtgt gagtggcaca gttcgttgaa ctctttcacc atatccagac gttgctgcgc
     1141 atcttcatca ctacgccaca gcaccagcag ttttaaggct ttgccaccgt cttgtttgat
     1201 ctgtagcgga ttgattttgc ggtcaataac cacgctatcg accggaatac cgttgccagg
     1261 aatgaactca tcggcggcga caatcatggc gcaacttttg gcaatcgcgt tttgctcaac
     1321 cacctggcgg tagcagaatt gttgatctac cagaatcgcc gaggcataag gcgagagggc
     1381 ctttgcagcg ttaactttga aatcagttaa aacgctatcg gctaccggag cgggtgcccc
     1441 agccgcggca aacatcatgc gcatggcttc gcgctgatcg accgccagca tggcaaaacc
     1501 gcccgatgcg cgcgtaatgt cgttgatggt gtacttattc attcatttat tcctttatcc
     1561 agtcgttaca tttttacttt ggcagtcatc ccggcactga cacggacctg ttccagaatg
     1621 gcggaccagt cctggcgacc gcgacccgct gcgcgcgcct ggctataaac ctcccgtgag
     1681 gcggccccca gcggcattgg cacatgcagc tggttggcga catcaagggc gatgccaaga
     1741 tccttatggg caagatcgat catgaaggcg ggagaaagat ctccgctgag gactttgttt
     1801 ggccaggaag tggtgaagtg acctttaccg gcggcggtac cgctcatcac tttgacggca
     1861 acatcgaagg gaagattcag ggcttcgcac aaaacggcgg cttctgccga aagcgcattg
     1921 agcgcgatgc tcatgtagtt gttgatgagc ttaacgcgga tccccatgcc cggaccgcca
     1981 gcgttgatca actcactgcc catcgccatc aggatcggtg tggcacgttc aacttgttca
     2041 gcggtgccgc cagccagcag taacagagta ccagtaatgg catttgcaga agtacgggct
     2101 accggaacat ccatcatgct gaagcctttg gcttgcatat cggcaatcaa tttatcggtt
     2161 tgcagcggat ggatggtgga catatcaatg accagcgcat cggtagataa gccttcgcaa
     2221 acgccgtttt caccgaacaa cacgttgcgc accagatcgc cattcggcaa catggtaatg
     2281 ataaattcgg catctttagc ggcctgcgcc gggttggcgg cgggagtcgc acctttgtct
     2341 accagatgcc gcacagcttc ggcgttcaca tcaaagacgc gaagttgatg cccttgctgc
     2401 aataaattgc tcgccattgg cgaacccatt tgtcctaaac cgataaatgc gattgctgcc
     2461 ataaccctct cctgaataca gtgatgtcac tttttgtcat ttatgacatg ctttgcttgt
     2521 ctgtttttga tcgcatttgt aatttatcgt caaaaaattg acagccgtca ctttttaaac
     2581 aattggtgaa attaaaatga acgcatccca aaatgtttaa ggaatgacca tgattcgtgt
     2641 tgcttgtgta ggtataaccg tgatggatcg catctattac gtggaagggt taccgacgga
     2701 gagcggtaaa tacgtggcga gaaattatac ggaagttggt ggcgggccag cggcgacggc
     2761 agcggttgcg gcggcaaggc tgggggcgca ggtcgatttt attggtcgcg taggtgatga
     2821 cgacgcgggc aacagcctgc tggcagagct ggaatcctgg ggcgttaaca cccgttacac
     2881 caaacggtat aaccaggcga aatcttcgca atccgccatc atggtggata ccaaaggcga
     2941 gcggataatc attaactacc ccagcccgga tctgctgcct gacgcagagt ggttggagga
     3001 aattgatttc tctcagtggg atgttgtgct ggcagatgta cgctggcacg acggcgctaa
     3061 aaaagccttc accctggccc gtcaggcggg tgtgatgacc gttctggacg gggacattac
     3121 gtcgcaggat atcagtgagc tggtggcatt aagcgatcac gcggcctttt cagaaccggg
     3181 tctggcgcgc ttaacgggcg tgaaagagat ggccagtgcg ctaaaacagg cacaaacgct
     3241 cacaaatggt catgtctatg tgacccaggg tagcgcaggc tgcgactggc tggaaaatgg
     3301 tgggcgtcag catcaaccgg ccttcaaagt tgatgtggta gataccacag gtgcgggtga
     3361 tgtttttcac ggcgctttgg cggtggcgct ggcaacaagt ggggatttag cggagtcagt
     3421 ccgcttcgcc agcggtgtag cggcgttaaa atgcacacgt cccggtggac gagccgggat
     3481 ccctgactgt gatcaaaccc gatctttttt gtcacttttt gtataaaatg ccagggtgat
     3541 ggtttttcga ggaattttca tgagccttac cgaactaacc ggtaacccgc ggcacgacca
     3601 actcctcatg ctgatcgccg agcgtgggta tatgaatatt gatgagctgg caaatctgct
     3661 ggatgtctcc acgcagacgg tccgccggga tattcgtaaa ttaagcgagc aaggcctgat
     3721 tacgcgccat cacggtggcg cgggtcgggc ttccagcgtc gttaatacgg cgttcgagca
     3781 gcgtgaggtt tcgcaaaccg agaaaaaaaa agcgattgcc gaagcggtgg cagactatat
     3841 tcctgatggt tcaacaatat ttatcaccat tggtacgact gttgagcatg ttgcccgggc
     3901 gttacttaac cataatcatt tgcggataat caccaacagc ctgcgtgtgg cgcatattct
     3961 ttaccacaac ccgcgctttg aagtgatggt gcccggcggt acgttgcgct ctcataatag
     4021 cgggatcatt ggcccttcag cggcgtcctt tgtggctgat tttcgtgctg attatctggt
     4081 aacaagcgtt ggggcgattg agagcgatgg cgcgttgatg gagtttgatg taaacgaagc
     4141 taacgtggtg aaaacgatga tggcgcacgc gagaaatatt ctgctggtcg ccgatcacac
     4201 taagtatcat gcttcggcgg cggttgaaat tggtaacgtg gcacaggtca ctgcgctctt
     4261 taccgacgag ctgccgcccg ctgcgctaaa atcacgctta caagacagcc aaattgaaat
     4321 cattcttccc caggaagacg cgtagatttt tgtgaccgtt aacctggctt cataccttgc
     4381 cacatagcca aacccatcct ttcccgctac agttaatttc ttgtggcgcg aaaggaggca
     4441 aaaatgctct atatctttga tttaggtaat gtgattgtcg atatcgactt taaccgtgtg
     4501 ctgggagcct ggagcgattt aacgcgtatt ccgctggcat cgcttaagaa gagttttcat
     4561 atgggggagg cgtttcatca gcatgagcgt ggggaaatta gcgacgaagc gttcgcagag
     4621 gcgctgtgtc atgagatggc tctaccgcta agctacgagc agttctctca cggctggcag
     4681 gcggtgtttg ttgcgctgcg cccggaagtg atcgccatca tgcataaact gcgtgagcag
     4741 gggcatcgcg tggtggtgct ttccaatacc aaccgcctgc ataccacctt ctggccggaa
     4801 gaatacccgg aaattcgtga tgctgctgac catatctatc tgtcgcaaga tctggggatg
     4861 cgcaaacctg aagcacgaat ttaccagcat gttttgcagg cggaaggttt ttcacccagc
     4921 gatacggtct ttttcgacga taacgccgat aatatagaag gagctaatca gctaggcatt
     4981 accagtattc tggtgaaaga taaaaccacc atcccggact atttcgcgaa ggtgttatgc
     5041 taaaaaccat tcaggacaaa gccaggcacc gtacccgtcc actatgggcc tggctaaaac
     5101 tactctggca acgtattgat gaggacaaca tgacaaccct ggcaggtaac cttgcctatg
     5161 tgtcgttgct ctcattagtg ccgctggttg ccgttgtttt tgcgcttttc gccgcttttc
     5221 ccatgttttc cgacgtcagc attcagttac gtcactttat ttttgccaac tttctgcctg
     5281 ctactggcga tgttattcag cggtatatcg aacaatttgt tgctaattcc aacaagatga
     5341 ccgccgttgg ggcgtgcggg ctgatcgtca ccgcgttatt gttgatgtac tccatcgata
     5401 gcgcgttgaa taccatctgg cgcagtaaac gagcgcgacc aaaaatttac tcgttcgccg
     5461 tgtactggat gattttaacg ctggggccgc tgctggcagg ggccagtctg gcgatcagtt
     5521 cctatttgct ctccctgcgc tgggcgagcg atctcaatac tgtcatcgac aacgtgctgc
     5581 gtatttttcc gttgctgttg tcgtggatct ccttctggtt gctgtacagc attgttccta
     5641 ccatccgcgt acctaaccgc gacgcgattg tcggcgcgtt tgtcgccgca ctcctgttcg
     5701 aagcaggaaa gaaaggtttc gcgctttata tcaccatgtt cccgtcatat cagctcattt
     5761 acggtgtgct ggcggtgatc cccattctct ttgtttgggt ctactggaca tggtgtatcg
     5821 tcttgcttgg cgcggaaatt actgtcactc tcggggaata ccgcaaacta aaacaagcag
     5881 ctgaacaaga agaagacgac gaaccatgat tgcattaatt caacgcgtaa cccgtgccag
     5941 cgtcaccgtg gagggagaag tgacgggcga aattggcgcg ggacttttgg tgttattggg
     6001 tgtcgaaaag gatgacgacg aacagaaagc aaaccgtctg tgcgagcgtg tgctcggcta
     6061 ccgcatcttt agcgatgccg aaggcaagat gaatctcaac gtgcaacagg cgggcggcag
     6121 tgtactggtg gtttcccagt ttaccctcgc cgcagatacc gaacggggga tgcgcccaag
     6181 tttctccaaa ggcgcatcac cggatcgcgc agaggcgtta tatgactatt tcgtcgaacg
     6241 ctgccgccag caagagatga acacgcaaac aggacgcttc gctgccgata tgcaggtatc
     6301 gctggtcaat gatggccccg tgacattctg gttgcaggta tgagccagct tccagggttt
     6361 tcacgggaaa caagagagag tatcgctatg tatcaccttc aggttccaca aacagaagaa
     6421 gagttagagc gttactatca gtttcgctgg gaaatgttgc gtaagcccct gcatcaacca
     6481 aaaggttcgg aacgcgacgc gtgggatgcg atggcgcatc accagatggt cgtcgacgag
     6541 cagggtaatc tggtggcggt aggccgattg tatattaatg ccgacaatga agcgtccatt
     6601 cgctttatgg ccgttcaccc ggacgtgcag gacaaagggt taggaacgct gatggcgatg
     6661 accctggagt cggtggcgcg tcaggaaggc gttaagcgcg tgacctgtag cgcccgtgaa
     6721 gacgcggtgg agtttttcgc caagctgggg tttgttaatc agggagaaat caccacgcca
     6781 accaccacgc cgattcgcca ttttttgatg attaagcccg tcgccactct ggatgatatt
     6841 ttgcatcgcg gcgactggtg cgcgcagctg caacaggcgt ggtacgaaca tatcccgctt
     6901 agtgaaaaaa tgggcgtgcg tattcaacaa tataccgggc aaaaatttat caccaccatg
     6961 ccggaaaccg gcaatcagaa tccgcaccat acgctgtttg ccgggagttt attctcgctg
     7021 gcgacgctca ccggttgggg gcttatctgg ctgatgctgc gtgaacgcca cctcggcgga
     7081 acgattattc tggcggatgc gcatatccgc tacagcaaac cgattagcgg taaaccccat
     7141 gcggtagccg accttggtgc cttaagcggc gatctcgacc gtctggcgcg cggacgaaaa
     7201 gcacgggtgc agatgcaggt cgaaatcttt ggcgacgaga cgccgggtgc agtgtttgaa
     7261 ggcacgtata tcgttctgcc cgcgaagcca tttggcccgt atgaagaggg cgggaacgaa
     7321 gaagagtagc ttatggagag gggcggtgct gcctctctca ttcattccgg tcatttacat
     7381 cagtccctga ctattccggt tatattgaat aagaacttta taaattttta ttcttcgttg
     7441 gcaccatacg tgtcttgcta catcactgtt caggagcatt taaatattat ttgtagagct
     7501 gagtggcaat tattgcagat aatgcttaac tcatcatctt agttcaggtt tgagcgattt
     7561 ttgatagtca aaaccccctc tggtgccata cttttttcat aaatattttt aaaatctata
     7621 gctcacaaac attatgcaaa gataatgggc tggcatacat tgtcagtatc attcatttac
     7681 tgctctgtat gttatgaatc attatgttgt atgtttgctc ttaaaccgtg ggaaggaggc
     7741 tataatggtt gccaatatta atctcattaa acaagaaagt tattctgttg ttaatttaga
     7801 aaaacaacta atgagtaata aatcaccgaa gcaaacgcta ttaaatcaaa tttcactttg
     7861 ccttaaaatg gaaggtgagg agcaactgtg cgattgtgtt aacgaactga agcatggttt
     7921 gtcgagtaat aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa
     7981 agtaccagaa agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg
     8041 cttactcaac gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt
     8101 ttatggcaaa gatatggtga caaagtccgg ggatatattg attgatatac aattaaataa
     8161 ggatggcagt ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta
     8221 taatattgac cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa
     8281 atcctggtga cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg
     8341 aatgcacggg taaaaatcat caaattaatg ttaaagccaa attgccacaa gaacgaagtg
     8401 aaaaagttga ggatatgtgt gaaagaatga aaggtggcat ctctatgttt aaccacacca
     8461 caaaaacagc tgataatata gagcataatt tgcagctaag ttttctcgtg ggttcacgtc
     8521 cgtcggtcac caataaatac tcagcaaaag atg
//
LOCUS       DADLSB010000160         8546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.160, whole genome shotgun sequence.
ACCESSION   DADLSB010000160 DADLSB010000000
VERSION     DADLSB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.160"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            128..1534
                     /gene="fliD"
                     /locus_tag="JN331_003425"
     CDS             128..1534
                     /gene="fliD"
                     /locus_tag="JN331_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY7261476.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     ALAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            1559..1969
                     /gene="fliS"
                     /locus_tag="JN331_003426"
     CDS             1559..1969
                     /gene="fliS"
                     /locus_tag="JN331_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY7261477.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            1969..2334
                     /gene="fliT"
                     /locus_tag="JN331_003427"
     CDS             1969..2334
                     /gene="fliT"
                     /locus_tag="JN331_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY7261478.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            2412..3899
                     /gene="amyA"
                     /locus_tag="JN331_003428"
     CDS             2412..3899
                     /gene="amyA"
                     /locus_tag="JN331_003428"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7261479.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(3933..4346)
                     /locus_tag="JN331_003429"
     CDS             complement(3933..4346)
                     /locus_tag="JN331_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7261480.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            4533..5738
                     /gene="yedE"
                     /locus_tag="JN331_003430"
     CDS             4533..5738
                     /gene="yedE"
                     /locus_tag="JN331_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY7261481.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            5735..5968
                     /gene="yedF"
                     /locus_tag="JN331_003431"
     CDS             5735..5968
                     /gene="yedF"
                     /locus_tag="JN331_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY7261482.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            6075..6745
                     /locus_tag="JN331_003432"
                     /pseudo
     CDS             6075..6745
                     /locus_tag="JN331_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(6785..7993)
                     /gene="tnpB"
                     /locus_tag="JN331_003433"
                     /pseudo
     CDS             complement(6785..7993)
                     /gene="tnpB"
                     /locus_tag="JN331_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
     gene            8001..8430
                     /gene="tnpA"
                     /locus_tag="JN331_003434"
                     /pseudo
     CDS             8001..8430
                     /gene="tnpA"
                     /locus_tag="JN331_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2086 a         2196 c         2180 g         2084 t
ORIGIN      
        1 gacgaaatac tcttttctct gccccttatt cccgctatta aaaaaaacaa ttaaacgtaa
       61 actttgcgca attcaggccg ataaccccgg tattcgtttt acgtgtcgaa agataaaagg
      121 aaatcgcatg gcaagtattt catcgctggg agtcgggtca ggtctggatt taagttcaat
      181 ccttgatagc ctcaccgccg cgcaaaaagc gacgctaacc cctatttcaa atcagcaatc
      241 gtcgtttacc gctaaactta gcgcctacgg tacgctgaaa agcgcgctga cgactttcca
      301 gaccgccaat actgcattgt ctaaagccga tcttttttcc gccaccagca ccaccagcag
      361 caccaccgcg ttcagtgcca ccaccgcggg taacgccatc gccgggaaat acaccatcag
      421 cgtcacccat ctggcgctgg cgcaaaccct gaccacgcgc accaccagag atgatacgaa
      481 aacggcgatc gccaccagcg acagcaaact caccattcaa caaggcggcg acaaagatcc
      541 gattaccatt gatatcagcg cggctaactc atcgttaagc gggatccgtg atgccatcaa
      601 caacgcaaaa gcgggcgtaa gtgcgagcat cattaacgtg ggtaacggtg aatatcgtct
      661 gtcagtcaca tcaaatgaca ccggccttga taatgcgatg acactctcgg tcagcggtga
      721 tgatgcgcta caaagtttta tgggctatga cgccagtgcc agcagcaacg gtatggaggt
      781 ctcggtcgcc gcccagaatg cgcagctgac ggtcaacaac gtcgccatcg agaacagcag
      841 caacactatc agcgacgcgc tggaaaacat caccctgaac ctgaacgatg tcaccacggg
      901 caaccagacg ctaaccatca ctcaggacac ctccaaagcg caaacggcga ttaaagactg
      961 ggtgaatgcc tataactcgc taatagatac cttcagcagc ctgaccaaat acaccgccgt
     1021 agatgcggga gctgatagcc agagttctag caatggcgca ctgctcggcg actccacgct
     1081 gcggacgatt cagacgcagt tgaagtcgat gctgagtaat accgtcagtt cttccagcta
     1141 taaaacgctg gcgcagattg gtatcacgac cgatcccagc gatggcaaac tggaactgga
     1201 tgccgacaaa ctcaccgctg cactgaaaaa agatgccagc ggcgtaggtg cattgattgt
     1261 tggcgatggt aaaaaaaccg gcatcacaac caccatcggc agcaacctga ccagttggct
     1321 ttcgacaacg ggcattatta aagccgctac cgatggcgtt agtaaaaccc tgaataagtt
     1381 aactaaagac tacaacgccg ccagcgatcg cattgatgcg caggtcgcgc gctacaaaga
     1441 acaatttacc caactggacg ttttaatgac ctcgttaaac agcaccagca gctacttaac
     1501 gcagcagttc gaaaacaaca gtaattccaa gtaatcatta ttcatcggga gacaggtcat
     1561 gtacgcggca aaaggcaccc aggcctatgc acaaattggc gtcgaaagcg ccgtaatgag
     1621 cgccagccaa cagcagttgg tcaccatgct atttgatgga gtgctgagcg cactggttag
     1681 agcgcgactg tttatgcagg acaacaatca gcaaggcaaa ggcgtctctt tgtcaaaagc
     1741 gatcaacatc attgagaacg gactgcgggt gagtcttgat gaagagagca aagacgaatt
     1801 aacccaaaac ttaattgctc tttatagcta tatggtcagg cgtttgctgc aagccaattt
     1861 acgcaacgat gtctccgcag tcgaagaggt ggaagcatta atgcgtaata ttgccgatgc
     1921 ctggaaagag tctttactct ccccttcttc gattcaggac ccagtctgat gaacaatgca
     1981 ccgcatttgt atttcgcctg gcaacaactc gtcgaaaaaa gccagctcat gttacgcctg
     2041 gcaacggaag aacaatggga cgaactcatc gctagcgaaa tggcgtatgt gaatgcggtg
     2101 caggagatcg cacacctgac tgaagaggtt gccccgtcca ctacgatgca ggagcagctc
     2161 cgcccgatgc tgcacctgat tctcgacaac gaaagcaagg taaagcagtt attacagatt
     2221 cggatggatg aactggcgaa actggtcggt cagtcatcgg tgcaaaaatc ggtgttaagt
     2281 gcctatggcg atcagggcgg ctttgtgctg gctccgcagg ataacctctt ttgaatctta
     2341 atgagccgat ggctcgcgaa taatccgatt acggctacgc ttctaatatt ccccttgaat
     2401 ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt tcactggtat tacccggaag
     2461 gcggtaagct ctggcctgaa ctggccgagc gcgcagacgg ttttaatgat attggtatca
     2521 atatggtctg gttgccgccc gcctataaag gcgcatcggg cggctattcg gtcggctacg
     2581 actcctatga tttatttgat ctaggtgagt ttgatcagaa aggcagcatc cctactaaat
     2641 atggtgataa agcacaactg ctggccgcca ttgacgccct gaaacgtaat gacattgcgg
     2701 tgctgctgga cgtggtagtc aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg
     2761 tgcagcgtgt aaatgctgat gaccgtacgc aaattgacga agaaatcatt gagtgtgaag
     2821 gctggacgcg ttacaccttc cccgctcgtg ccgggcaata ctcgcagttt atctgggatt
     2881 tcaaatgttt tagcggtatc gaccatatcg aacaccctga tgaagatggc atttttaaaa
     2941 ttgttaacga ctataccgga gaaggctgga acgatcaggt tgatgatgaa ctcggtaatt
     3001 tcgattatct gatgggtgag aatatcgatt ttcgcaatca tgccgtgacg gaagagatta
     3061 aatactgggc gcgctgggtg atggaacaaa cgcaatgcga cggctttcgt cttgatgcgg
     3121 tcaaacatat tccggcatgg ttttataaag agtggatcga acacgtacag gaagttgcgc
     3181 caaagccgct gtttattgtg gctgaatact ggtcgcatga agtcgataaa ctgcaaacgt
     3241 atattgatca ggtggaaggc aaaaccatgc tgtttgatgc gccgctgcag atgaaattcc
     3301 atgaagcatc gcgcatgggt cgcgactacg acatgacgca gatcttcact gggacgctgg
     3361 tggaagccga tccttttcac gccgtaaccc tcgttgccaa ccacgacacc caaccgttgc
     3421 aagccctcga agcaccggtc gaaccgtggt ttaaaccgct ggcgtatgcc ttaattttgt
     3481 tgcgggaaaa tggcgttcct tcggtgttct atccggacct ctacggtgca cattacgaag
     3541 atgtcggtgg tgacgggcaa acctatccga tagatatgcc aataatcgaa cagcttgatg
     3601 agttaattct cgcccgtcag cgtttcgccc acggtgtaca gacattattt ttcgaccatc
     3661 cgaactgcat tgcctttagc cgcagtggca ccgacgaata tcccggctgc gtggtggtca
     3721 tgtcgaacgg agatgatggc gaaaaaacca ttcatctggg agagaattac ggcaataaaa
     3781 cctggcgtga ttttctgggg aaccggcaag agagcgtagt gacagacgaa aacggcgaag
     3841 cgaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt tatcgaagag gtgatttaaa
     3901 tccatccccg gcggcaagcc ggggagattt cattacggca gtggcgtcgg taacggcgct
     3961 ttatccagcg cggcggcaca ctctgccgtc gggcgatcca cacgctccat cgtcatacca
     4021 tcgtactcaa tggtgttacc gtcacgctca acctcgtaca gctcacgttt tacggtaacg
     4081 ttagtgagat catcagacat caatgtcaac ttacccggca cggcaatcac ccgctgccac
     4141 tgacggcaat ccagggtatc gccctcttta gtgacaatca ggctgcctat cgcttccgga
     4201 ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc ccgccagcca gtccggcgcg
     4261 ggggttttca cgacattgtt atagttttcg acttcggcac agcccgccag caacatgagc
     4321 gcgcctgcaa ttgcgagttt tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg
     4381 gcattaaagc tctgatgtcg ccagcctttc caggttcaga gagaaatttg atctaactat
     4441 tatttttgtg aattattacc ccgaaaataa atttgcatgg tgtatgattc gcggctcttg
     4501 tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc aaacacgcct
     4561 ggttgattaa attctgggca cccatccctg cggtcatcgc ggcgggtatt ctctccactt
     4621 actattttgg cattactggc accttttggg ctgtcacggg tgaatttacc cgttggggcg
     4681 gtcagctcct gcagctgttt ggcgtccatg ctgaagagtg gggttatttt aaaattatcc
     4741 atctggaagg atcgccatta acccgcatcg acggcatgat gatcctcggt atgtttggcg
     4801 gctgctttgc tgctgcgctg tgggccaaca atgtcaaact acgcatgcca cgcagccgta
     4861 tccgcattat gcaggccatc attggcggca ttatcgccgg ttttggcgcg cgtctggcaa
     4921 tgggctgtaa cctggcggcg ttctttaccg gcattcctca gttctcgctg catgcctggt
     4981 tctttgccat cgccactgcc attggttcat ggtttggcgc gcgctttacc ctgctgccca
     5041 tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc ctcaccgctg acgcaaaaac
     5101 cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggc atgctgggct
     5161 gggcgctgct cacggcgatg aaccaaccaa aactggggct ggcaatgctg tttggcgtcg
     5221 gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt
     5281 ggatcaccgg acgtacccat atggcgaaag caatcattat cggtatggcg gtgagtgcca
     5341 tcgggatctt cagttacgta cagttaggcg ttgaacctaa aatcatgtgg gcgggaccaa
     5401 atgcggtaat tggtggttta ctttttggtt ttggcatcgt gctggcaggc ggctgcgaaa
     5461 ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca
     5521 acgtgatcgg ctcaacgatt ctggcgtatt actgggatga tttcgctccg gcgctggcca
     5581 ccgactggga caaaatcaac ctgctgaaaa cttttggtcc gatgggaggc ctgctggtga
     5641 catatttgct gctgtttgct gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc
     5701 gccgtgcggc accgcagact gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg
     5761 ccttgatatg gtgggtgaac cctgccctta tccggcagtc gcaacccttg aggcgatgcc
     5821 gcaattaaaa aaaggtgaga tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa
     5881 tattccactg gatgcgcgta atcacggcta cacggtgctg gatattcagc aagacgggcc
     5941 gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtggccc acagcgggaa
     6001 catcgttacc tctgacacat agattttacc cgccccctct cctattcttt tcagtatgat
     6061 ctcaggaggt tgctatgtgt ggacgctttg cccaatccca aacgcgtgag gattaccttg
     6121 cgcttctcgc ggaagatatt gaacgcgata ttccctacga tcccgaaccc attggcagat
     6181 acaatgtcgc gccgggaacc aaagttcttc tgctcagtga acgtgatgaa caccttcatc
     6241 tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgccg ctgattaacg
     6301 cccgcgtaga aactgcctca accagtcgca tgtttaaacc gctctggcaa catggtcggg
     6361 caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa aaaagcctta
     6421 ttttatctat cgcgctgacg gacaacctgt ttttatggcc gcgataggta gcataccgtt
     6481 tgagcgtggt gacgaagccg aaggattttt gatcgtcact gcggcggcag atcaaggtct
     6541 ggtagatatt catgaccgcc gcccgctggt tctgtcgcca gaagctgcac gggaatggat
     6601 gcggcaagac attggtggta aagaagcctc agaaatcgct accagaagct gtgtgccagc
     6661 aaaccagttc acctggcacc ctgtgtcgcg cgcggtgggt aatgtcaaaa accagggcgc
     6721 ggagttaatt caacctgttt gttgacatcc tccacgccct gaatgacgag gacccctgct
     6781 acgttcaggc tgtcgcctga atcatttcgg tgggttcctg cttcaacgag cggcctgact
     6841 gcaccatctc tccacaggca agaacggcgt gccccgccgc taaaatgtta cgagcgccat
     6901 ttacatcggc gttcgctgta tatccacata cctggcatct gaatttactt tgtgacaggc
     6961 gattttcttt cgctgtatga ccacagcacg cgcaacgctg gcttgtgtac gctggcggaa
     7021 cagcaagcac ctgaccgcca cgccagagct gcttgtactc aagctggcgg cgcatttcat
     7081 accagccctg atccagtatc gaacggttta aacctgattt tgcccggaca ttgcgccccg
     7141 gctggctgac cgtacccgct gctgactttg acatgttgct gaccttcaaa tcctcaatga
     7201 ctatcattac gtggtttttg ctgacggtcg ttgtgacttt gtgaaggtag tccctgcgga
     7261 tatttgcgat acaggaatgc agtcgctgta ttttgcgttt ctgcttctgc cagttgttgc
     7321 tgaatttgac cttgcggctt aactgtcgct gaagtctcgc cagcttcttc tggtttttct
     7381 gaaaactgtt tacaggctca aagactgtgc catctgacag cgaggcaagt ttagccacgc
     7441 cagcatccag cccgaccatt gatgctgaag ggtgaaccgg agttgatact tcactttctg
     7501 tctgaatact gatgtaccac ttaccgcagg actggctgac agtgacattt ttcacaacac
     7561 ccgtgacctg acggctgttc cggtagcgca tccagccaag ttttggcaga aaaatacggc
     7621 tgttttcctg agcgagctta acgccttgcg gatagcggaa tgcatcattc tgtcaccgct
     7681 ttttgaatca ggggaacgcc gaccgcttct ggaaaaagtt tttgcaggcc tgctcagggt
     7741 ctttcagtga ctgttgcaat ggctgtgacg gagcatcttt aatccattgc gtttcagtgg
     7801 catttttcca ctcaaccaat caggaagcca ttttcgcgta agggatgtct ttgttaccgg
     7861 ccttatgatt ctcattctga cgtgccagag tacgactgaa aacgaaacga catgcgcctg
     7921 cgaagcgcct catttcacgc gcctgttgac caccgggtct taaccggaat ttgaatacct
     7981 gtagtcgttt cacagggcaa attatacttt ggtctgtgaa aaaaactgat atccgacgcg
     8041 gaaggcactg tgttttcctg atgcatgctc accgggtctt tgttaccaag taccgacgcc
     8101 agatttttga ccatgacgcg acagaaaaac tacgtactta cttttcaaat gtatgtgctg
     8161 attttgaagc tgaactggtt gaaatggatg gcgaaccaga gcacgtccat ctgttaatta
     8221 actatccccc caaactggcg atatccagtc tggtaaacaa acagcctcaa aggcgtatcg
     8281 ggtaggttac tgcgacggga tcgaccagat attgctgcca ggtattaccg caaagatgtt
     8341 ttgtggcgtc ccggctattt tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc
     8401 caatacattg aacaacagca aacacctggt caggtgaaaa aacgagcctt atatccccgc
     8461 cctgaaggac gggcttttac ggtgcactgg ataagcctgg agattatcta cacctgcatg
     8521 gacatcattg tactatgccc cgtaca
//
LOCUS       DADLSB010000161         8498 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.161, whole genome shotgun sequence.
ACCESSION   DADLSB010000161 DADLSB010000000
VERSION     DADLSB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.161"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..567
                     /locus_tag="JN331_003435"
                     /pseudo
     CDS             1..567
                     /locus_tag="JN331_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            982..1164
                     /locus_tag="JN331_003436"
     CDS             982..1164
                     /locus_tag="JN331_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261483.1"
                     /translation="MRCEMLHRYVRRKCSFRRSSASSLTDSLRSVVRLRRAVSAHAKT
                     DIRLSTESGDNTGKNM"
     gene            1181..1416
                     /locus_tag="JN331_003437"
                     /pseudo
     CDS             1181..1416
                     /locus_tag="JN331_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1441..1575
                     /locus_tag="JN331_003438"
     CDS             1441..1575
                     /locus_tag="JN331_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7261484.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(1820..2929)
                     /gene="fepE"
                     /locus_tag="JN331_003439"
     CDS             complement(1820..2929)
                     /gene="fepE"
                     /locus_tag="JN331_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY7261485.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFCLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKPSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(3349..3717)
                     /locus_tag="JN331_003440"
                     /pseudo
     CDS             complement(3349..3717)
                     /locus_tag="JN331_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4495..5186)
                     /locus_tag="JN331_003441"
                     /pseudo
     CDS             complement(4495..5186)
                     /locus_tag="JN331_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            5477..6997
                     /locus_tag="JN331_003442"
     CDS             5477..6997
                     /locus_tag="JN331_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAY7261486.1"
                     /translation="MFLISIIQMFSVMIIMLNIKIKDAFRAIILLLLLLLSMVISIEI
                     SSIISIGEYIPTLALANISETNSVGWKIKLLPFLVFLSFMIASLILKRLIKEKKIRNI
                     MVVVFLLSLLIPISPINAFFSSSYEVYSEAKEYNMSPHEISDIKSKYTKETVTNDSYS
                     HEIINGNGKNILVIFIEGMSSLVISKELTPNLYEFRKKSISFNNYYNHTAATFRGLRG
                     QLTSSYQMTGGYHKDNSGLGQASKVEIEHKLNTYNYITKLPIILEENGYHTYFQASNS
                     IDDPLSLMLSTLKFNHIYGREDYNKKSGELTDKESFDLLFKKLNEAKQPFFYGVYTVG
                     THLGLDSPDVKFYDGRNEYLNHFHQLDHWFGDFISHFNKTSLSKDTIVIITSDHASYP
                     SEDYKKTFKTDNTFFVDQIPLIIWSNGVEFKDINAHGLNSLSLTPTILDILGIKESHN
                     MFIGESLFEKEKNKNELNCTSAIGDLYVKTCNGKMEIEKRKEKTELIKQLQLFGDR"
     gene            complement(7066..7802)
                     /locus_tag="JN331_003443"
                     /pseudo
     CDS             complement(7066..7802)
                     /locus_tag="JN331_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009652796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
     gene            7871..8143
                     /locus_tag="JN331_003444"
     CDS             7871..8143
                     /locus_tag="JN331_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261487.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMHPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            8124..>8498
                     /locus_tag="JN331_003445"
     CDS             8124..>8498
                     /locus_tag="JN331_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7261488.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPDCPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLN"
BASE COUNT         2474 a         1922 c         1837 g         2265 t
ORIGIN      
        1 ggaaacgggg gcaggaaccc ggtatcttct gcgccatcca cacgtatggc cgtcgtctca
       61 actggcatcc gcatgtacat gtcgaaaaaa acggccggcg ggaggaatac ggcgcgctac
      121 ctgggtcgtt atctgaagaa gccgccgata gcggcctccc ggctggcaca ttacaacgga
      181 ggggcgagcc tgagcttccg ttacctggac cacaaaacgg gagaaacggc gacggaaacg
      241 ctgacacagc gtgagctggt cgcgaggctg aaacagcaca tcccggagaa gttttttaag
      301 atggtgaggt acttcgggtt ccttgccaac cgggtgtgtg gagagaagct gccgcaggtg
      361 taccgtgctc tggggatgga taaaccggaa ccagtggcga aggtgtgcta tgcacaaatg
      421 gtgaagcagt tcctgagtcg tgacccgttc gaatgcgtgc tgtgtggcgg ccggatggta
      481 taccgccggg ccattgcggg gctgaatgtg gaggggctga agaaaaacgc gcgggatatc
      541 agtctgctga ggtatatgcc ggcctgacac aggctaagtg tgcccgggaa tcggtgcaag
      601 tataaaaaac agcctgttgc ggtataagaa tcgaccatgc agagagagaa aacaccggag
      661 tggagggaaa agcagaaaag ctcacgtggc ataaggcgtg gacagcggta tcgtctggtt
      721 tttcaatttc ctatacgtga actccagaac agcatccaga ttcgctttga ggccgaaagt
      781 gaaacaggaa cataacgaag ctgacggagg cggtcacggc tttcccgtgg gcgttttcgt
      841 cagcagacaa gcccgtcagg gcgcgtcagg gggttttagc gggtgtcggg gcgcagccat
      901 gacccagtca cgtagcgata gcggagtgta tactggctta attatgcggc ggcagagcag
      961 atggtgagag aacagcgaca tatgaggtgt gaaatgctgc acagatacgt aaggagaaaa
     1021 tgcagcttcc ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1081 ctgcggcgag cggtgtctgc tcacgcaaag acggatatac ggttatccac agaatcaggg
     1141 gataacaccg gaaaaaacat gtgagcaaaa aagaggcccc gaaaaaaggc cgcgccggtg
     1201 gcgtttttcc ataggctccg cccccctgac aagcatcaca aaaatcgacg ctcaagtcag
     1261 aggtggcgaa acccgacagg acttaaagat accaggcgtt tccccctggt ggctccctcg
     1321 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt
     1381 cgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctgggctgt
     1441 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1501 tccaacccgg taagacacga cttaacgcca ttggcagcgg ccactggtaa acaggatgtg
     1561 cgagagatgt attgagagtt cttgaagtgg cagccttact gaggctacac tagaaggaca
     1621 gatttggtga catgtctcgc acaagactgt taccacggtt cagaagttcc ccaacttact
     1681 gaaccttcga aaaaccacct ccccaggtgg ttttttcgtt ttctagagta gaagactacg
     1741 gctgtaaggg aatgatctca agaagatcct ttgaactttt tataaaagtt gcttataaaa
     1801 tgatgcatca gcacaacttt tactcaatca cactcatctt tctggcctcc atggcatgac
     1861 gtaaaagtat atagccacat gcaagcatcc ctccaatgac agaagaaaga attacaatta
     1921 tggcattact tggcccatcc tttctgaccg gtaacgttgg gctcaattga tactgaaacg
     1981 gcataaaatc aacatccccc actttcgcca caaccaattg attcacaaca tattgacgat
     2041 ttcgcaactc cccactcaat tccgaaacat ccttgattga ttttttgatg ttcaatttag
     2101 ttgctatacc atcagaaccg agagccacag gaaaatctgg gtcatccttc ataatctgac
     2161 cattgctgta tacaggtttt tttattccag ccgcattagc aacctccagt gaataattga
     2221 ggcgtcgaat atctgcattc agctggtttc taatttttat ctcatccagt gcaagaattt
     2281 ctttttcaac attagtttta acttctagtt tatttcttat atcttccatc aaatccctta
     2341 caacaaggga agaaacataa ttaatatacc cagacaacac atcgtgagct tctttacttg
     2401 ttggagcagt aaaactcaga gtccatgata catacaatga tggcttgtcg tttttcttac
     2461 tggcattatc atcaatcgac ttcatatttt tactcaactc aacaattgca cgatgcagtt
     2521 cgagagcgct aatgttattc tttttaataa tctccataac ataaggagat gatctcatat
     2581 actcattcaa cgaattaacg gactgaaatt tcttaataaa cagagcaaat gcgctatcac
     2641 gattgatatc aaaatccact cccagaacat gaagcttgga aagctcctta tttaattcac
     2701 tccattgaac agactcagca ggagtaacca cagcagaact agtccatttt tgtggcaaaa
     2761 caaaagaaac aagaaagcca atacaagcaa aaaatagaat caccccaaca atttttattt
     2821 ttgctgccca taaaacgcta atcaagcaaa aaaggtcaat ttcttcatta ccattcggca
     2881 acatgttgta attagaaaaa tgttcgtcag agacctttga gattttcatt gaaaaaaaca
     2941 caatatatta acatatggtt tttttattct atcatatgtt aggaaaaaac ctaaaaacca
     3001 ttcagcagac atttaacata acattttatt gcgtgcaccg cctcagaggc tctcagggca
     3061 tttctgacgg gaaaccggca aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg
     3121 caggctctgt ttttactgca tgcatgagta tgcacgcaag tgcatagcgc gaaaattcac
     3181 cccattcagc cagggtaagt cctgtttccg gcattcttcc catccgaaaa cggtgaactg
     3241 ggacgcaatt tattgcgtgg ccgtgaagcc gtaaggcacg gtaggtaatt gcaggtatcc
     3301 gacaatttcg agtagaggat ccactatttc actacttaga attcaaaatc agaatccttt
     3361 cttcctgcta aggctatctc tgaatgactc aaccactctt tgagattctt cttctgctca
     3421 catgaagtca ttcccggaca gaaaaaaacc attgtcaggc ccactggctt cagagaaatt
     3481 cgtttctgca gcagacacag aatccacatc agcaagagac ggttcatctg tactttttct
     3541 caatctgtaa agagccttat caaacgcaac cagtgagata ttcaggccgt aacgttcatt
     3601 cagcacagtc acaatctctt ccctacggat accatttttc ttccaggttt ctatatcgtt
     3661 cttggaatcc ctgacagccg caatggctgt tcttggacca gggttaaaat tcgccatata
     3721 ccgcccataa tgtcttaaat ttgtctcata atagtccaaa aataagattg aaacgcaaaa
     3781 aatgtcttac aattatctta ttgttgtcct ttccggttca tgtatccatg aaccgcctga
     3841 tgtggaacgc tttttatcga tctcatgcgc agccaaagat agattcattc tttaaaagtc
     3901 aggttacatt atcccttaaa tggtcataca ccatacgcaa ccgcgatttt catcgcaatt
     3961 gcatgaatta aaaggtaaaa actgaagtcc ccgtttattt atgtaatacg caacgcatta
     4021 caaccacctg taattacatg aaaataaaga aataagatat aataccggcg tattaccacg
     4081 tacgcgggta agcgaaaaag ctataacgct tcccggccga caggcggccc aaaatccacc
     4141 ggggcgtgca ggttctcttc agtaacctgc accattaaat caaaaaggtc atattcgccg
     4201 ggaagctgcg tatcagtcga atccagttgt tcatcatgtt cagatggcat ggggtgatat
     4261 ccagtagctg agaggtggtt agcgggtttc gggacgcagc cctaaaccag tcacgtagcg
     4321 ctagcggagt gtatactgac ttaggcggta gtgtagtatg acagattctg tggttacggc
     4381 aaagtaacag ccctgtctag gagagggctg ttatctgata taggcttgtt tttttctcac
     4441 cgtttttgcg agcgcttttt cctgccaaga ggtaggtagg gtaatgaatc caacttactg
     4501 atagtgtttt atgtttagat aatgcccgat gactttgtca tgcagctcca ccgattttga
     4561 gaacgacagt gacttccgtc ccggccttgc cagatgctgc ctcagattat gccgctcaat
     4621 tcgctgcgta tatcgcttgc tgataacgtg cagcttttcc ttcaggcggg attcataaag
     4681 cggccatccg tccgtcatca ataccaccac gtcaaaggcc gatagcaggc tcagaagacg
     4741 ctccagcgtg aatattgtgc gttcaccaaa aacgtgggcc acgacagtcc tgcgtaccct
     4801 gtcatacgcg taaaacagcc agcgctggcg tgatttagcc ccgacgtagc tccactgttc
     4861 gtccatttcc gcgcagacga tgacgttcct gcccggctgt atgcgcgagg ttaccgactg
     4921 cggcctgagt tttttaaatg gcggaaaatc gtgttgaggc caacgccaat aaagcgggcg
     4981 gttgcccggc atccgacgcc attcatggcc atatcaatga ttttctggtg tgtaccgggt
     5041 tgagaggcgg tgtaagtgaa ctgtagctgc catgttttac ggcagtgaga gcagagatag
     5101 cgctgatgtc cggcagtact tttaccgtta cgcacaacgc cgtcagtagc ggaacaggag
     5161 ggacagctga tggaaatgga agccaccgga gcacctcaaa agcaccgtca tccactaaat
     5221 cagtaagttg gaagcatcac cgcactagca gacagttaat taaaaaagta taatccctct
     5281 ccggatttat tgaataatgg agaattatgc tgttttctat gtcttattta gtcaaatgcg
     5341 cttatcaata ttgtgataag ctcccgagtc taaacttaac ttactgattt taggttgagg
     5401 aagaccggca tttttgtaga tccacggcat aatatctatt tattcaacaa cgccccccaa
     5461 aaaaaggttt ttataaatgt ttttaatttc aatcattcaa atgttctcgg ttatgattat
     5521 aatgcttaat ataaaaataa aagatgcatt tagagcaatt attctgcttt tacttctatt
     5581 actatccatg gttatatcta ttgaaataag cagcattata tcaataggtg agtacatacc
     5641 cacacttgca ctagcaaata tatcagagac aaatagtgtt ggctggaaaa taaaactact
     5701 accttttctt gtgttcttgt catttatgat tgcatcattg attttgaaaa gattgataaa
     5761 agaaaaaaaa ataagaaata taatggttgt tgttttttta ttatcattgt taataccaat
     5821 atctccaata aatgcttttt tttcttcatc atatgaagtt tattcggaag caaaagaata
     5881 caatatgtca ccacatgaaa taagtgatat aaagtcaaaa tacacaaaag aaacagtaac
     5941 aaacgacagt tactcacatg aaataataaa tggaaacgga aaaaatattt tagtaatatt
     6001 tattgaaggt atgtcttcat tggtaatatc taaggagcta accccgaatc tatatgaatt
     6061 tagaaaaaaa tcaatatcat ttaacaacta ttacaaccat accgcagcta catttagagg
     6121 tttaagagga cagttaacct catcatatca aatgaccggt gggtaccata aagataattc
     6181 tggccttggc caagcatcta aagtagagat cgaacataaa ctaaatactt acaattacat
     6241 aacaaaatta ccaatcatac ttgaggaaaa tggttaccat acatattttc aagcatctaa
     6301 ttcaatagat gacccattga gtttaatgct atcaacttta aagtttaatc atatatacgg
     6361 aagggaggac tataacaaaa agtcaggtga actcacagac aaagagtctt tcgatttatt
     6421 atttaaaaaa ttaaatgagg ctaaacaacc tttcttctat ggtgtttata ctgtaggtac
     6481 acaccttggc ttagatagcc ctgatgtaaa gttttatgat gggagaaatg aatatttaaa
     6541 tcactttcat caattagacc attggtttgg tgattttata tctcatttta ataagacaag
     6601 cttatcgaaa gatacaattg tcataatcac aagtgaccat gcaagttacc catcagaaga
     6661 ttacaaaaaa acattcaaaa ctgataatac tttttttgtt gatcagattc cattaataat
     6721 atggagtaat ggtgttgagt tcaaagacat taatgcacac ggtttaaatt cactatcttt
     6781 aacgccaaca atactagata ttcttgggat caaggagtca cacaacatgt tcataggtga
     6841 gtctctattt gaaaaagaaa aaaataaaaa tgaactcaac tgtacatctg caataggtga
     6901 cctgtatgtg aaaacgtgta atggaaaaat ggaaatcgag aaaagaaaag aaaaaacaga
     6961 attaataaaa caattgcaac tttttggtga tagataatta aaaggcggtt ttaccccatg
     7021 ttgattatta gtccacgtgg ggttagttta aaatgataat aaaacttact ttttttcatt
     7081 cttgagccgc ctgtagaatg tggctcgact aacacctaaa gactcccatt ctttttttaa
     7141 agttatatga attaatatta aaactacctt taggccgtcc tcctgaattg cttagctttc
     7201 ctcctttacc ccctctcttt ctttgaattt ctgaacggtg cgtatttttt acatactcaa
     7261 taaaacgatc ctctgaaaaa ttattcgctg tccattttga gatgcttttt gatattccaa
     7321 gaacttcatt ttcacttaat ggtgatacga attgtaaatt gtaggcacag gctcgttcat
     7381 agcaggcttg taaccactgc tcatactcag gccacccctg acggatggca cgatatgccc
     7441 acttgcgggt tttgtcgaac agagtgcagt tacggcctaa accataatcg gcggcaattt
     7501 tccagtgact gtggttcgga ttcttgcaaa ttagccccga atatcccgtg tcagctccca
     7561 attttctacg tagcgcattc tctacgcgca gctgcgtagc gtaacggttt catttttccg
     7621 tcaggtgctg tgcgtataga ggtttccagc gcataaagca ggtgcgcgtg tccgttttcg
     7681 gggttggtga tggtcagcgt aggtgcggga gcgttgcggt cgctccagtc aatggctgcg
     7741 ccgatgcggt caacatcaaa tcccagccag aacatggcgt gaggctgatt gaaccacggc
     7801 agcctggcgg ctgcaaatcg gggtgtggca gaatatgagc tttcagagta accatccaga
     7861 atgagttccc atgtcacaca acctggagca ccagaaagtc cacaccagaa tggtgaaaga
     7921 ggttctgaaa gccgtcgcca gagccaataa tcacccttat aagtccgtat ttgcagactt
     7981 tatcaccgga catccctcct gcacggtctg tttctgggaa accttccaca aaatgcaccc
     8041 ggattcaccg tacgaatacg tcacgttctg ccatacctgc cgtcgttttg atttatacga
     8101 aacagaagcg gagatgaagg cggatgaccc gaagtggtgg tgattttcag ccccgaccgc
     8161 tgaaacgcct gttcacagcc aaccagtgct ggacatcctt cctggatgcg ggcggtctgc
     8221 gcgatatcga ggttgaggct gtcacaaaaa tgctggcctg cggcacgcgg atactgggcg
     8281 tgaaggaata caactgcgat aaacctgact gcccccacgt cagatacgtc actaactcat
     8341 gcggcagccg tgcctgcccg tcctgcggaa aaaaggccac agacctgtgg atagcgacac
     8401 agctgaatcg tcttcctgac tgcgactggg tacacctggt cttcaccctg ccggacacgc
     8461 tgtggccggt gttcgaaagc aaccggtggc tgctgaat
//
LOCUS       DADLSB010000162         8488 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.162, whole genome shotgun sequence.
ACCESSION   DADLSB010000162 DADLSB010000000
VERSION     DADLSB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.162"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(204..950)
                     /gene="cutC"
                     /locus_tag="JN331_003446"
     CDS             complement(204..950)
                     /gene="cutC"
                     /locus_tag="JN331_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY7261489.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(964..1530)
                     /locus_tag="JN331_003447"
     CDS             complement(964..1530)
                     /locus_tag="JN331_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7261490.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1746..3479
                     /gene="argS"
                     /locus_tag="JN331_003448"
     CDS             1746..3479
                     /gene="argS"
                     /locus_tag="JN331_003448"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY7261491.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAAYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3656..4144
                     /locus_tag="JN331_003449"
     CDS             3656..4144
                     /locus_tag="JN331_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HAY7261492.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4262..4654)
                     /locus_tag="JN331_003450"
     CDS             complement(4262..4654)
                     /locus_tag="JN331_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAY7261493.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4654..6036)
                     /locus_tag="JN331_003451"
     CDS             complement(4654..6036)
                     /locus_tag="JN331_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAY7261494.1"
                     /translation="MSMGHAAESYTLLTIGDGLVAQIPALVISTAAGGIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVNFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6033..6653)
                     /locus_tag="JN331_003452"
     CDS             complement(6033..6653)
                     /locus_tag="JN331_003452"
                     /inference="COORDINATES: protein motif:HMM:NF012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261495.1"
                     /translation="MILLILSMMVLPLPAFILDLLFTFNIALSIMVLLVAMFTQRTLE
                     FAAFPTILLFTTLLRLALNVASTRIILMEGHTGAAAAGKVVEAFGHFLVGGNFAIGIV
                     VFVILVIINFMVITKGAGRIAEVGARFVLDGMPGKQMAIDADLNAGLIGEDEAKKRRS
                     EVTQEADFYGSMDGASKFVRGDAIAGILIMVINVVGGLLVGVLQLA"
     gene            complement(6678..7871)
                     /gene="flhB"
                     /locus_tag="JN331_003453"
     CDS             complement(6678..7871)
                     /gene="flhB"
                     /locus_tag="JN331_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAY7261496.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLVLLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRICQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVHLLIFRCRKPWILLTRNRPMSNLAAMLRLPA
                     NLKST"
     gene            8107..>8488
                     /locus_tag="JN331_003454"
     CDS             8107..>8488
                     /locus_tag="JN331_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY7261497.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         2183 a         2276 c         2065 g         1964 t
ORIGIN      
        1 gttttagcaa tttaaatccc ataatcaatg tgaatataaa aaatattttt agcacaacgc
       61 tatgcgcact gtcacccgct caacagatca cggttatcgt tcgtttttta tactgctcag
      121 ggataaaaaa aggcccctgt tgaaattgca ggggcctggt acgagcaagc atcatattgg
      181 gcgacatgat gcaacggtaa aaatcatttg gcctgatggc gttcaatgat tcctttcatt
      241 tcagcaaccg ccgccccgtc tacgatataa cgcgaatact cgtccgcgtg ttcatctgat
      301 gacatggaca atccttgatt acgataacgc atcggtgagg cttgccacgc tcccgcggag
      361 ctatggactt ccagcactcc ggcatcgagg aagtggtgca ggttttctgc acggactcct
      421 gctccagcca taatgattgg agcatcacga tgggcaataa gttccataat ttttgataaa
      481 ccttgcagtg cgtctgattt ttgccctgat gtcagtaccc gggcaatgcc caattccgca
      541 agattattga gtgtatataa agggttagcg cacatatcga aggcgcgatg aaaagtcact
      601 gccagcggac cggcagcagc cattattttt tccattcgtg gcatatcgac attcccgtca
      661 acatcgagaa cgcccgtcac cagtccagga aaacctaatt cgcggaccgt gcgcacatcc
      721 tcaagaatgg cggcaaactc accgtcgctg tagcaaaaat caccaccgcg tgggcgaatt
      781 atcggatgca cagggatcgt cacccgctgg cgcacggatt tcagtacccc caacgacggc
      841 gttaagcccc cctcttttgg ggctgcgcat aattcaactc tgtctgcgcc gttttgctgc
      901 gccgttagtg cacattccat gctgtaacag caaatttcca gtaatgccat ttttgctcct
      961 taattacgct gactgctcgc tggcaacgat ctcttcaatg gaccacggat gaaacttaat
     1021 agttgttttg ccatcggtga ccgccagcgt cggattcggt aatcgctcat gctcaccttt
     1081 tggtgaactg gttttaactg agatccccgg taggctgagt ccttcatcag aaagcaatgc
     1141 cagcgcacgg gcattcagcg tttctggatc acccggcaga acgatttcaa tatgttccca
     1201 accttcgtgt gggtaacgtt tttccccggg ccacggtagc tccacattgg aaaactgcca
     1261 gtgcgcaacc tgtaccggtt catgcaattt aaacagacaa atcggtctgc cattgatcat
     1321 attttctgac aaaagctcgc cacactgttc aaaaccgcga cgccagcgct cagcggtgac
     1381 gttttgatgg caacgcaaag aaatgtgatc ggcagtcagc ggagtgatat ccaaccccag
     1441 acggcgggaa agttcatcta atgcgtgggt aaatcgcggt aaatccgatg caatatcctg
     1501 cagctcgtcg atagattgcc agttcgccat aatcactctt cgtctttcag taaaagcgtt
     1561 aatttaccct gttgccctgt gccaaccaac cgctgatttc acgccgcttc tgatgcaata
     1621 gtgaaaacgg caatacgcca cgcgcacgtt gctgacgaaa acagccattt gcagtatact
     1681 cccgccctaa tttctttaac tggtgcgggc aatttttgct cgcttcatca atgtaaggta
     1741 ttccggtgaa tattcaggct cttctctcag aaaaagtccg tcaggccatg attgcggcag
     1801 gcgcgcctgc ggattgcgaa ccgcagattc gtcagtcagc aaaagttcag ttcggcgact
     1861 atcaggctaa cggcatgatg gcagttgcta aaaaactggg tatggcaccg cgacaattag
     1921 cagagcaggt gctgactcat ctggatctta acggtatcgc cagcaaagtt gagatcgccg
     1981 gtccaggctt tatcaacatt ttccttgatc cggcattcct ggctgaacat gttcagcagg
     2041 cgctggcgtc cgatcgtctc ggtgttgcta cgccagaaaa acagaccatt gtggttgact
     2101 actctgcgcc aaacgtggcg aaagagatgc acgtcggtca cctgcgctct accattattg
     2161 gtgacgcagc agtgcgtact ctggagttcc tcggtcacaa agtgattcgc gcaaaccacg
     2221 tcggcgactg gggcactcag ttcggtatgc tgattgcatg gctggaaaag cagcagcagg
     2281 aaaacgccgg tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat gcgaaaaagc
     2341 attacgacga agatgaagag tttgccgagc gtgcacgtaa ctacgtggta aaactgcaaa
     2401 gcggtgacga atatttccgc gagatgtggc gcaaactggt cgacatcacc atgacgcaga
     2461 accagatcac ttacgatcgt ctcaacgtga cgctgacccg tgatgacgtg atgggcgaaa
     2521 gcctctacaa cccaatgctg ccaggaattg tggcggatct caaagccaaa ggtctggcag
     2581 tagaaagcga aggggcgacc gtcgtattcc ttgatgagtt taaaaacaag gaaggcgaac
     2641 cgatgggcgt gatcattcag aagaaagatg gcggctatct ctacaccacc actgatatcg
     2701 cctgtgcgaa atatcgttat gaaacactgc atgccgatcg cgtgctgtat tacatcgact
     2761 cccgtcagca tcaacacctg atgcaggcat gggcgatcgt ccgtaaagca gcctatgtcc
     2821 cggaatccgt accgctggaa caccacatgt tcggcatgat gctgggtaaa gacggcaaac
     2881 cgttcaaaac ccgcgcgggt ggtacagtga aactggcaga cctgctggat gaagccctgg
     2941 agcgtgcacg tcgtctggtg gcagaaaaga acccggatat gccagctgac gagctggaaa
     3001 aactggctaa cgcggttggt attggtgcgg tgaaatatgc ggatctctcc aaaaaccgca
     3061 ccacggacta catcttcgac tgggacaaca tgctggcgtt tgagggtaat accgcgccat
     3121 acatgcagta tgcttacacg cgtgtattgt ccgtgttccg taaagcagaa attaacgaag
     3181 agcaactggc tgcagctccg gtaatcatcc gtgaagatcg tgaagcgcaa ctggcagctc
     3241 gcctgctgca gtttgaagaa accctcaccg tggttgcccg tgaaggcacg ccgcatgtaa
     3301 tgtgtgctta cctgtacgat ctggccggtc tgttctctgg cttctacgag cactgcccga
     3361 tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct gaaactggca caactgacag
     3421 cgaagacgct gaagctgggt ctggatacgc tgggtattga gactgtagag cgtatgtaat
     3481 cgatttttcg tgagagtgaa gcctgatcag ggttagccga tcaggctttt ttattgccat
     3541 ctaaatgtat tctgaaaacg gacatgccat tgttttctca ctgttgagta agaggccaga
     3601 agcgtaatat ccaggcccag gaaaacgata acggttcaat ttaaggaata ccgcagtgtt
     3661 taaatttctt gtattaacat taggcattat ctcttgccag gcttacgcag aagatacggt
     3721 tatagtaaac gaccatgaca tttcagccat caaagattgt tggcaaaaaa attcagatga
     3781 tgatactgac attaacgtga tcaaatcatg cctgcgacaa gaatacaatc tcgtcgatgc
     3841 gcaattaaat aaagcctatg gtgaagctta tcgttatata gaacaagtgc cacgcacagg
     3901 tgcaaaaaaa cttgatactg aacaacttaa cttgcttaaa aaatcacagc gagcctggct
     3961 ggattttagg gacaaagaat gtgaattaat cctttcaaat gaggatgttc aggatttaag
     4021 tgacccttat tctgaatcag aatggctatc atgtatgatc atacagacca atacgcgaac
     4081 tcgccagttg cagctatacc gtaactctga agatttttat ccaagccctt tgacaagagg
     4141 ataattcaca tctttttggc atgttttgtt gcaagctatt cctgataaat gattgcaaca
     4201 aaacatccag ccttttgact gagttattaa acatactcgc gagcgcgtaa ttttttgtct
     4261 ttcagcgata attcacaatc acttcattcc gttgtacctt tagcggtgga attaaccgcc
     4321 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
     4381 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
     4441 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
     4501 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
     4561 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
     4621 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
     4681 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
     4741 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
     4801 cgcacccagc atctcctgac gggatagcgc ttcctgagtt tgcgccagta aacgatccgc
     4861 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
     4921 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
     4981 ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc
     5041 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
     5101 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
     5161 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
     5221 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
     5281 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     5341 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     5401 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     5461 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     5521 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     5581 caacaactca ccatcctgct ggaaattgac catcgggatc agtcgataac ccacttccat
     5641 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     5701 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     5761 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     5821 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     5881 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     5941 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     6001 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     6061 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     6121 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     6181 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     6241 tgcttacccg gcataccatc gagaacaaag cgcgccccca cttctgcgat acgcccggca
     6301 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     6361 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     6421 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     6481 aacaacgtag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     6541 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     6601 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     6661 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcagcc
     6721 agattactca tgggtcggtt tctcgttaat aaaatccagg gcttccggca ccggaagatg
     6781 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     6841 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     6901 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     6961 ccaatttcac gaatacgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     7021 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     7081 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     7141 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     7201 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     7261 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     7321 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     7381 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     7441 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     7501 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     7561 agcgccacca gcaccacgcc gctaatcagc ggtagcagca ccagcatggc ttctctgatc
     7621 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     7681 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     7741 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     7801 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     7861 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     7921 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     7981 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     8041 cctatagaat cgccctccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     8101 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     8161 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     8221 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     8281 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     8341 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     8401 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     8461 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DADLSB010000163         8421 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.163, whole genome shotgun sequence.
ACCESSION   DADLSB010000163 DADLSB010000000
VERSION     DADLSB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.163"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(58..555)
                     /gene="rayT"
                     /locus_tag="JN331_003455"
     CDS             complement(58..555)
                     /gene="rayT"
                     /locus_tag="JN331_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY7261498.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(731..1344)
                     /locus_tag="JN331_003456"
                     /pseudo
     CDS             complement(731..1344)
                     /locus_tag="JN331_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            1636..2376
                     /locus_tag="JN331_003457"
     CDS             1636..2376
                     /locus_tag="JN331_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY7261499.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2347..3114)
                     /locus_tag="JN331_003458"
     CDS             complement(2347..3114)
                     /locus_tag="JN331_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY7261500.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3320..3898)
                     /gene="lpcA"
                     /locus_tag="JN331_003459"
     CDS             complement(3320..3898)
                     /gene="lpcA"
                     /locus_tag="JN331_003459"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY7261501.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4138..6582
                     /locus_tag="JN331_003460"
     CDS             4138..6582
                     /locus_tag="JN331_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7261502.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGVYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6625..7098)
                     /gene="ivy"
                     /locus_tag="JN331_003461"
     CDS             complement(6625..7098)
                     /gene="ivy"
                     /locus_tag="JN331_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY7261503.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7252..8022
                     /gene="yafV"
                     /locus_tag="JN331_003462"
     CDS             7252..8022
                     /gene="yafV"
                     /locus_tag="JN331_003462"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY7261504.1"
                     /translation="MPGLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCPYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            8132..8364
                     /locus_tag="JN331_003463"
                     /pseudo
     CDS             8132..8364
                     /locus_tag="JN331_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
BASE COUNT         2034 a         2117 c         2251 g         2019 t
ORIGIN      
        1 gttaaaacac gatttgtagg ccggataagc gtaagcgcat ccggcaccag cgcacagtta
       61 taaaatgata cgctccccgg cacttaaatc cgttatgtct cccgcccaat cgatgggata
      121 taaccctcgc gcgacatcgc gatggaacgt tgagaatggc caatcactca cctgctttac
      181 ccagccatgt ttctctggat ttatataaat ataatcaaca tgatggcgat aatcttttgt
      241 attgcgaatg gcatgctccc aaaaacgcgg ttgccagata tttttcaatc cgcaagcatg
      301 ggtgaactgc tttttaatct cccgccagcg agaggaaaaa tcatcatcgc tttcgggtaa
      361 tgtccatatg caatgcatat gctccggcaa aacgacccag gcgttaattt caaaaggtct
      421 gtctcgctta acgttaataa tggcgttacg gagcgtctgg aactgggtgg tcagaagatg
      481 gcttcgacga ttgcgtaaat ttaccgtgaa aaaccatgtt ccccccttga tgtaataacg
      541 acgatattcc gacatagcga ctcctttttc tcaacggtaa gaggaaaagg aaaggccgac
      601 aatgacgctg tgttatatcg ggaaagcggc tcgcaaaaac gcacagattt gtaggtatga
      661 taagacgcgt cagcgtcgca tcaggcatcc gtgcacaacc gccggatgcg gcgtaaacgc
      721 cttatccggc ctacaaaatc atctcttccg tcaaaatcct gcgcgcgcct aaaaaatggt
      781 cctgccagaa aggttcggct aaccggctta cccgaatggt ttccccggtg cgcagtgact
      841 caataaactg accgtcgccc agatacaccc ccatatggtc ggcaatctcg cggctgtgga
      901 tatggaaaaa tagcaaatct ccccggcgca ggtcgttatt cgccacaatc gttgcccgac
      961 ggtagtggta catctcattg gccgtgcgcg ggagtttcgc ctcaaggatc ttgttgtagg
     1021 cataaaaaac taacccacta caatcgaagc cttgatcggg ccgcgtgccg ccccagacgt
     1081 aaggcttgcc aagctgctgc tccaggcggt gaatcgccac ttcggtgatg ttatgcaggt
     1141 gatcgctgct taaaaagtgg ttgttttccg ccagcgcctg ccagcgtctg tcactcgcct
     1201 tgagcggcgc aggaaaccat tctggatgct gttttatctg ctcccggttg tggtggcgct
     1261 ggcgagtaaa gggagcaaaa cgcataacga aaacacgcct ggatgcaaaa atctgccgag
     1321 caagaacgaa gacatgaaag acataaaccg gctgaatgaa ggtgagaatt ttaaaagacc
     1381 ggaggagtaa acgggattat taattgctta tttgcatatt actattgaga tttcataatc
     1441 ttaggaaata aatgatacat gcgcttttcg gactattgaa aactgcccct gtgctaaatt
     1501 tgtaaaccca gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca
     1561 aatccagact attccgattt gggatccatt ttgccgtcct agcgtgtgta accgttttat
     1621 caaggaataa gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc
     1681 gtttcgtttg ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat
     1741 aagcagcagt tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc
     1801 gatctttacg tcaaaatggg cgagcaatat caactgctcg acagttataa aatctgtaaa
     1861 tattccggcg gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt
     1921 tatagcgtcc agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt
     1981 ggattcccca atgcttatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac
     2041 ggcgattgtg tatccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc
     2101 cagttcgtta ctggtgcgct ggtgtttggt cagccgagcg tgcaggtgag tatttaccca
     2161 ttccgcatga ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg
     2221 gagcagctga agccgggcta cgactacttt gagcaaaccc gtaagccacc gaccgtttct
     2281 gtggtcaatg gccgttacgt ggtcagcaag ccgttaagcc acgaagtggt gcagccacaa
     2341 ctggcatcaa actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatttt
     2401 ttgccaggtt tcattgcccg tcagcggctg tgtcgcaatc accgtgacca catcattcgg
     2461 tgtggtctgc gagctgaagt cgatttccac atcctgatcc agcaacgttg ccacgccaaa
     2521 cggcgcgcgg cgggtgatcc agtgtaaatt agtcgagcaa tacgccatta catagcgccc
     2581 gtccgaaagc agcatgttga aaacgccctt cgcccgcagt tcatccgcca gtgaggcgat
     2641 atatttaaac actgccgcca tgttgcccgg cgtgcgcggg taacgctgcg ttaatttatg
     2701 cagcagccag caaaaggctt tttcgctgtc ggtttcaccg accgggcgga agttgccggt
     2761 ttccagtgat ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca
     2821 taactcgcgg gtaaatgggt gagtattttc cagcgccacc tcgccccgat tagcctggcg
     2881 aatatgagcc accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg
     2941 ggaattaaag ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt
     3001 aatgccccag ccatctttat gtggcccggt tccaccacca cgctgtacaa gtccggtgaa
     3061 actaaagcag atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa
     3121 cctccacaac agcgggagcg catggacccc ggcgaagatt gatttaagaa ttttcaggtc
     3181 ggatgcggcg taaacgaatt attcggccta cggttcgggt acagatttgt aggcatgata
     3241 agacgcgtca gcatcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct
     3301 tatccggcct acgccagact tacttaacca tctctttttc aattaactgg atcaggatat
     3361 ggatcacttt aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa
     3421 tttcgatatc cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt
     3481 tcatcccctt ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg
     3541 tggagatccc cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa
     3601 tatcattgaa accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa
     3661 tcgccgggta gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca
     3721 tagcgtcgca atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc
     3781 tgtctgctaa caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa
     3841 agttagccag cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga
     3901 ggatatcctt cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc
     3961 gagaagtacg ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag
     4021 caacaatgtg agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc
     4081 actacaacca tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg
     4141 atgattttga gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg
     4201 agcttattta tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt
     4261 ctgtggtcgg cgtgggtact ggtgcctctg accattatcc tcgtgccatt taactttgcg
     4321 cctatgcgta agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg
     4381 ccgatgtcgc gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac
     4441 ttgttccagg gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc
     4501 gctgaagagc aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat
     4561 ttccagatta ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag
     4621 catcgcttct tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat
     4681 gcccagtctc gtgtgctgca aaaactctct ggcgtgagcg ggatcctggc gattaccgtc
     4741 ggcgtgccaa actcattagg cccgggcgaa ctgttgcaac attacggcac tgacgagcag
     4801 aaagatcact atctgccgcg tctggcgcgt ggtcaggaga tcccctgctt tgcactgacc
     4861 agcccggaag cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc
     4921 gaatggcagg gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctacattacg
     4981 ctggcaccga ttgcgaccgt gcttgggctg gcgtttaaac tctccgaccc ggaaaaatta
     5041 ctcggcggtg cagaagattt aggcattacc tgtgcgctga tcccaaccac cacgccgggc
     5101 gtggaaattg gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc
     5161 ggtaaagatg tcttcgtgcc gatcgattac atcatcggcg ggccgaaaat ggccgggcaa
     5221 ggctggcgga tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac
     5281 tcaaccggcg gcgtgaaatc ggtagcgctg gcaacgggcg tgtatgctca cattcgccgt
     5341 cagttcaaaa tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc
     5401 gggaacgctt atgtaatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc
     5461 gaaaaacctg ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag
     5521 tcgattattg atgcgatgga tattaccggc ggtaaaggca ttatgctcgg gcaaagcaac
     5581 ttcctggcgc gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt
     5641 ctgacccgca gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg
     5701 gaagagatgg aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa
     5761 catatcggtc acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt
     5821 ttaaccagca gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc
     5881 ctgagcgcca acctcgccct gctttctgat gtctcgatgg cagtgctggg cggcagcctg
     5941 aaacgtcgtg agcgtatctc ggcacgtctg ggggatattt taagccagct ttacctcgcc
     6001 tctgccgttc tgaagcgtta tgacgacgaa ggccgtaatg aagccgatct gccgctggtg
     6061 cactggggcg tacaggatgc gctgtatcag gcagaacagg cgatggacga tttactgcaa
     6121 aacttcccga accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt
     6181 cattatctgg caccgtctga caagctggat cataaagtgg cgaagatttt acaagtgccg
     6241 aacgccactc gttcacgtat tggtcgcggt cagtacctga cgccaagcga gcataacccg
     6301 gttggtttac tggaagaggc gttggtggat gtgattgccg ccgacccgat tcatcagcgg
     6361 atctgtaaag agctgggtaa aaatctgcca tttacccgtc tggatgaact ggcgcacaac
     6421 gcgctgacga aggggctgat tgataaagat gaagccgcta ttctggtgaa agcggaagaa
     6481 agccgtctgc gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg
     6541 gtaaagttgc cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa
     6601 aggctccgtt tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag
     6661 gctgccggtc aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac
     6721 attcagccag gtgagtttct cttgcgacgt tttctcatca atagccgaga acagccccgt
     6781 catctgatta gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg
     6841 tttgcacgcg ctcatcacct gatacgtctc atctcccaac gttacggttt gtgcgggagt
     6901 ataagtaccg cctttcatca cccatgcagg tagcttatgc ccttgcacca tctgattaaa
     6961 cgcagctttg gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat
     7021 tgcactggtg gcgatgacca gtgcggcgac tgtcgttatt gccttaaaca tcattcctcc
     7081 cgagcttatc ctgcccatgt tattaacctc ctaaagcctg gcgataacac gataatttgc
     7141 aaatcagata tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc
     7201 ctgataaggt gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt
     7261 ttgaagatta cgcttttgca gcaaccgttg gtgtgggtgg atggtcctgc caacctgcgc
     7321 cattttgatc gtcaactgga aggtattacc gggcgcgatg tgatcgttct accggagatg
     7381 tttaccagcg gctttgccat ggaagcggca gcttcgtcgc ttgcacaaga tgacgtagtg
     7441 aactggatga cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta
     7501 caaacggagt ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat
     7561 ttttatgata agcgtcatct gttccgcatg gcagatgagc atctgcatta taaagcgggc
     7621 aatgcgcgag tgattgtgga atggcgcggc tggcgtattt tgccgctggt gtgctacgac
     7681 ttacgttttc cagtgtggtc gcgcaatctc aacgattatg acctcgccct gtacgtcgcc
     7741 aactggccag ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag
     7801 aatcaggcgt atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgcccttat
     7861 cgcggggaca gccgggtgat taatccgcaa ggcgagatta tcgctaccgc cgacgcgcat
     7921 aaggcaacgc gtattgatgc ggagctgtcg atggtggcat tgcgggaata tagagaaaag
     7981 tttccggcat ggcgggatgc ggatgagttt aggttgtggt gagtgggggt tgtgtttagg
     8041 gccgggggct gggctagatt acgaaagccc ctaacttaaa cacaatatta cgccagaagt
     8101 caccatttcc aaaacacagc gtcgctgtac cgtgggttat ctgcccaacc gcagcgacac
     8161 gccggtgatc aaaatcagcg ccaaatggct gagaaaagcg ggatttgaga tcggcacggg
     8221 cgttaccgtg aagatctcgg agggatgttt gatcctgctt gggataacaa cgaagtacag
     8281 gaactgcgca gggaacttta tcaggttaaa cagtcagtta aggggatgcg agacgggatg
     8341 tttagtgtgc tgaacgaaag ttaaataata aaaagccgga aagatctgga agaaatttcc
     8401 ggttattatc gttgctatat c
//
LOCUS       DADLSB010000164         8320 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.164, whole genome shotgun sequence.
ACCESSION   DADLSB010000164 DADLSB010000000
VERSION     DADLSB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.164"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..1631
                     /locus_tag="JN331_003464"
                     /pseudo
     CDS             1..1631
                     /locus_tag="JN331_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1600..3336)
                     /gene="aceK"
                     /locus_tag="JN331_003465"
     CDS             complement(1600..3336)
                     /gene="aceK"
                     /locus_tag="JN331_003465"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY7261505.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHCSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3520..4824)
                     /gene="aceA"
                     /locus_tag="JN331_003466"
     CDS             complement(3520..4824)
                     /gene="aceA"
                     /locus_tag="JN331_003466"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY7261506.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4854..6455)
                     /gene="aceB"
                     /locus_tag="JN331_003467"
     CDS             complement(4854..6455)
                     /gene="aceB"
                     /locus_tag="JN331_003467"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY7261507.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRSVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6724..7653)
                     /gene="metA"
                     /locus_tag="JN331_003468"
     CDS             complement(6724..7653)
                     /gene="metA"
                     /locus_tag="JN331_003468"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY7261508.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7810..8253
                     /locus_tag="JN331_003469"
     CDS             7810..8253
                     /locus_tag="JN331_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY7261509.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2100 a         2050 c         2073 g         2097 t
ORIGIN      
        1 ctcactcact tcaacaaaaa atgcaaaaac cagactttgg cgttgatttc cagccgtcct
       61 gaggggcgtt gcgttgctgc ctgcggtgat ttcgggctag ttatgaaagc atattttgac
      121 aagatggaat caaatggcat cagtgttatg gcagccatat tactggtgga taaccatgct
      181 ttgacggtcc ggctaagaat aaagaacaca actgaaggat gtacccatta cgtggtttcg
      241 gtttatgctc ctaatgtaac taacgataaa ataagaatta tgagcgaaag caaagaggat
      301 attaaacact attctctgat ggattttatg aatgtagatt atagcctcct gaaatggtca
      361 aatgatcatg ttattaacca atctgttgca ataattccag cacttccgaa agaacagcta
      421 ttgatgttaa aaggaactgt ggatgaaata acccctccat tatcaccagc aacgatgaat
      481 ttgctaatgg caattggtca gaatcaccaa cttacgcaac tgatgattca gctccagaaa
      541 atgccagaac tacatagaac agaaatgttg actgcctata atagtattaa cctccccggt
      601 ttatatttgg ctataaatta tggcaatgcg gatatcgttg agactatttt caattcattg
      661 tcagaaacag gatatgaagg gttactctcg aaaaaaaatc tcatgcatat tctggaggca
      721 aaagataaaa atggtttttc tggattattt ttagcgatat cacgtaagga taaaaatgtt
      781 gtaacctcga ttctgaacgt cttacctaaa ctggccgcaa cacatcattt agataatgaa
      841 caggtgtata aattcctgag tgccaaaaat agaacgtcca gccatgtttt ataccatgtt
      901 atggcgaatg gtgatgccga catgctgaaa attgttttgg tcgcgttacc tttgttaatt
      961 cgcacatgtc atttgactaa agaacaggta ctcgatctcc tgaaggcaaa ggatttttat
     1021 ggttgcccaa gactatacct ggcgatgcaa aatggacata gcgatatcgt gaaagttatt
     1081 ctcgaagcat tgccctgtct ggcccaggaa attaacattt cagcttccga tattgtagat
     1141 cttctgaccg ctaaaagtct tgcgcgcgac acgggtttgt ttatggccat gcagcgcgga
     1201 catatgaacg ttattaatac tatttttaac gcattaccca ctctttttaa tacgtttaaa
     1261 ttcgataaaa aaatatgaag cccctcctcc tggcaaataa ttctaatgaa tatcccggtt
     1321 tgttttcagc gatacagcat aaacaacaaa acgttgtaga gacggtttat cttgctttat
     1381 ctgaccatgc acgcctgttt ggatttaccg ctgaagatat tatggatttt tggcaacaca
     1441 aagcgccaca aaaatactct gcctttgagt tggcttttga atttggtcac cgggttattg
     1501 ctgaattaat ccttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc
     1561 ctcgatacat tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata
     1621 ccgtacgcta aatctttgcc tgcgccgata cgcataaaca tcttccacat gcccttcacg
     1681 tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc
     1741 aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac
     1801 atcgcccggc gagacgctgt accacggttc gctggcaagt tcgtcttccg gatagcgcgg
     1861 cggcgggatg tcgcggaaat tcacttccgt catgtagcaa atttcatcgt aatcataaaa
     1921 aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc cagggaaaat
     1981 gttagcagcg gcaagctggc gaatagcgtt accgtattct tcaatggcgt cgcgcaactg
     2041 ctgaccttcc acttgttcca gccagatatt gagcggcacc atccgccgct cgatatagag
     2101 atggcgaatt acaatctgct caccaagatc ggtgattttt tccgctgctt cctggagcag
     2161 taattccatt aataccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg
     2221 ggtgtccgcc attcggccca cacggtcgtg ctctttcacc agttgatagc aggcacgaac
     2281 gtgagcggca gacatctctt tctgcggcgc gaacttgtct ttgatgactt tgaatacccg
     2341 atcaaagccc ggcagcgtaa acaccaacat caccattcca cgaatacccg gcgcttcaat
     2401 gaactgctca ttacagccct gtagataaac gagatattcg cggtagcttt cggttttggc
     2461 gtgcttctgg cagccgatag ccatatacaa ttcagcagtg gttttacctg gcagaatttc
     2521 ccgtagccac tcgaccagcg ctgcgggcag cggtgcataa accataaaat aagaacgcgc
     2581 aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg caggtatcaa taaataactc
     2641 gccgtcgtcc gtctggtgga tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag
     2701 tttgcccacc agccaggcgg ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg
     2761 actttccgcg aggttgtctg tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat
     2821 gtcacggctt ttattctgcc agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag
     2881 tagagattcc cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg
     2941 aaagcggcgc tctggctgag agctaaagat aaaaagccgc tcgggagtaa gcgagcagtg
     3001 gtcaaataac cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta
     3061 atccggcaac agccgcgtgt aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact
     3121 ttggccgtta gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag
     3181 atggatacgg tttttcatcg cctgctggac agcatgccag tcggcctgtt cgaaacgctg
     3241 ctgcgccccg gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat
     3301 ggtttgagca atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag
     3361 aattgccgga tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg ttgtaggcat
     3421 gataagacgc gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtaaat
     3481 gccttatccg gcctacagtc agcaacgttt gtaggttgct tagaactgcg attcttcagt
     3541 ggagccagtc agcgcggtga ctgaagacgt gccgccctga ataatagtcg tcactttatc
     3601 gaagtaacct gtacccactt cctgctggtg agatacgaag gtatagccat ctttcgcggc
     3661 ggcaaattcc ggctgctgca ctttctcaac atagtgcttc ataccttcgc cctgcgcata
     3721 agcgtttgcc aggtcgaaca tgttaaacca catgctgtgg atacctgcca gggtgatgaa
     3781 ctggaacttg tagcccatat ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc
     3841 gaggtttttc tgccagttga acgacggcga gcagttataa gccagcagtt tgcccggata
     3901 tttcgcgtgg atagcttgtg caaagcgacg cgccagttcc agatccggcg tggaggtttc
     3961 acaccagact aggtcagcat atggcgcata cgccaggcca cggctgatcg cttgctcaat
     4021 gcccgcatga gtacggaaga agccttcact ggtacgctcg ccggtaataa attcgctgtc
     4081 atacgggtcg caatctgagg tgatcagatc cgccgcatca gcatcggtac gtgcaaccag
     4141 cagggttgga acgcccgtca cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc
     4201 ttcctgagtt ggcactaaaa ctttgccgcc catgtgaccg catttcttca ctgacgccag
     4261 ctgatcttcg aagtgaactg ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa
     4321 ggcattcagg acaccgccaa aaccggcttc cgcatcggca acgatcggca ggaagtaatc
     4381 gacatagcgc ggatcgcccg gctcaatgcc cacggaccat tggatctgat cggcacgacg
     4441 gaaggtgttg ttgatccgct ccaccacagc tggcaccgag tttgccggat agagcgactg
     4501 atccggatac atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata
     4561 gactgcttca atacccgctt tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag
     4621 gctgttgatg tagccttttt tcgactcacc gtgcagcaga cgccacattt tcgctgcgcc
     4681 cagttgcgcc agcgtgcatt caggattgac tgaaccgcgt aatttcacca catcttccgc
     4741 actgtatggg cgagtaatgc cttcccaacg cggttgagtc cactctttct gtaattcttc
     4801 aatttgttgt gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct
     4861 aacaggcggt agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt
     4921 tccatcaagc gtgcggcatc gtcaaaacgg ccctgggaga aacgttcttc gcccagttcg
     4981 ctggcaatga ctttcatctc ttcgcccagc atctggcgga acaaggcttt ggtcaccggt
     5041 ttgccattgc tcaacgtttt ttgatgatgg atccactgcc agatcgaggt acgggaaatt
     5101 tcagccgtcg ccgcatcttc catcaggcca taaatcggca cgcagccgtt gccggagatc
     5161 cacgcttcga tgtactgcac agccacgcga atgttggcgc gcataccttc ttcggtgcgt
     5221 tcaccatcac aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcaat
     5281 acttcaagct gatttttacg ggagccgaga atgtcgttga ataccgccat tgccgtgtcc
     5341 gcaaggcctg ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc cagcgcttta
     5401 tccgctttta ctttgttgag cacctggtta ttacgctctt catctttgct cggaataaac
     5461 gctgccatgc cgcccatcgc aaaagcaccg cgtttatggc aggttttaat cagcaggcgt
     5521 gagtaagcat tcaggaatgg tttatccatc gtcacggcct gtctgtctgg caggacacga
     5581 tcgggatagt ttttcaacgt tttgatatag ctgaagatgt aatcccagcg accgcagttc
     5641 agaccaacaa tatggtcgcg cagcgcgtga aggatttcgt ccatctggaa cacggcgggc
     5701 agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct
     5761 gcatagctga agacttcgct ccaccaggcc gcttcctgcc aggactgggt tttcggcaga
     5821 tagaaatagg gaccgctgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc
     5881 gcaaaatcaa acaggctacc ggggattgcc tcaccacgcc aggtgacatg tttttccggc
     5941 aagtgcagac cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctgataaatt
     6001 ttgcctgctt cattggtata gctgatggta ccgttaaccg catcgcgcag gttaatttgc
     6061 ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact
     6121 ttcacattgg cgttcagtgc gttgatcacc atcttgcgct ctaccgggcc agttatctct
     6181 acgctgcggt cttctaagtc cgcaggaatc ccgcggattt tccaatcagc atcgcgaatg
     6241 gaagccgttt ccgaaataaa atcaggcaac gttccgttat caatatcttg ctgctgctga
     6301 atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga
     6361 aattctaccg cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttgtgaaa
     6421 gccagttcat cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt catggatccg
     6481 aaaacttccc ctctgtgaac gaaggaacgt tgtgcgcttt tcgttcagca caactaagac
     6541 tactcattta aaatgcaaaa tcaaaaacaa tttccatttt aattaaaaat acttgataac
     6601 ttattgataa caatatgaat aaaatttatt cacttgctga ggtgcgtttc ggaaataaaa
     6661 aaggcacccg aaggtgcctg aggtaaggtg ctgaatcgct taacgatcga ctatcacaga
     6721 agattaatcc agcgttggat tcatgtgccg tagatcgtat ggcgtgatct gatagacgta
     6781 atagttgagc cagttggtaa acagcaaatt accgtgacta cgccagctcg ctcgcggttt
     6841 gttttgcgga tcgttgtgcg ggaaatagtt atacggtaca tccgggtcta gtccggcttc
     6901 cacatcgcgg aaatattcct gcgccagcgt ttgcgcatca tattcgggat ggcccgtcac
     6961 aaaggcaatg cgcttatctt tgctagcaaa cagatatgca tccccttctt ccgtctctgc
     7021 cagaatttcc agatcggtgt aatcacgaat caacgctgcc ggaaagtcag catagcgcga
     7081 atgcggtgcc agaaatgaat catcaaagcc acgcgtcaga agcgcatgag gatggagaat
     7141 atgatgctcg taaacgccag agagtttgtc ggtgcgagtt tgcttaggaa tgccgtagag
     7201 gatattgagc gcggcctgta ccgcccagca gacaaacagc gtcgaggtga cgtgatcttt
     7261 cgaccactcc agcacctgtt tgatctgcgg ccagtaagcg acatcattaa actccaccag
     7321 gcccagcggc gcaccggtta caatcagacc gtcaaagttc tgttcctgaa tatcttcaaa
     7381 gttacagtag aagttgttca gatgctctgc gggcgtgttg cgcgattcac gggaatcgat
     7441 gcgcaacagc tgaatatcga cctgcaaagg tgagtttgaa agcaggcgca gaaactgatt
     7501 ttcagtttca atcttcttcg gcatcaggtt aaggatcaga accttaagtg gacgaatttc
     7561 ctgaccagac gcacgagaag ttgtcatcac aaagacgttt tcttcacgca agaaattgac
     7621 ggcgggtagc tcgtccggca cacgaatcgg cataacctga ttacctcact acatacgctt
     7681 atacgtttag acatccagat agctgaagat aaccagaaaa tttgccaatg tcgagcctgc
     7741 atgttgaagg tgagaaagtt tcaaggaaaa ggctacgtta gaatataaga atgacgaaaa
     7801 ggagagaaaa tggttattag tattcgccgc tcacggcatg aggaagggga ggaactcgtt
     7861 gcgatttggt gtcgatctgt cgatgccacc cacgattttt tatcagcaga gtatcgggcc
     7921 gagctggaag agctggtgcg ttcctttctg ccggaagcac cgttgtgggt cgcggttaat
     7981 gaacgggagc agccggttgg gtttatgttg ttaagtgggc agcatatgga tgcgctgttt
     8041 atcgatcctg atgtgtgcgg ctgcggcgtg ggtcggatgc tggtggagca tgcgctctcg
     8101 atggcaccgg aactgacaac caacgttaat gagcaaaatg agcaggcggt tgggttctat
     8161 aagaaggtgg gttttaaggt tacgggacgc tctgaggtgg acgatttggg gaaaccgtat
     8221 ccgttgctga atctggcgta tgtgggggaa taagccgccc ctgccgtgcc tgcccaagct
     8281 ggagaagacg cgccagcgtc gcacccggca tttttttcac
//
LOCUS       DADLSB010000165         8167 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.165, whole genome shotgun sequence.
ACCESSION   DADLSB010000165 DADLSB010000000
VERSION     DADLSB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.165"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1312
                     /locus_tag="JN331_003470"
     CDS             <1..1312
                     /locus_tag="JN331_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7261510.1"
                     /translation="DKVKYPTGVENHGGTLRIWFNFKGKRVRENLGVPDTAKNRKIAG
                     ELRTSVCFAIRTGNFDYAAQFPDSPNLKAFGVNKKEITVKELEEKWLDLKRMEISANA
                     FNRYESIARMMVPKIGGNRLVSAVTKEELLYIRKDLLTGYQNPTKGKAPVKGRSVVTV
                     NYYMTTIAGMFQFAADHGYIEANPFEGIKPLKRARAEPDPLTRDEFIRLIDACRHQQT
                     KNLWSLAVYTGMRHGELVSLAWEDIDLKAGTITIRRNYTKLGEFTLPKTEASTNRVVH
                     LIQPAISVLKNQAEMTRLGKQHNIKVQLREYGRSVNHECTFVFNPQVVRKSKQVGFIY
                     KVDSIGDSWETAIKRAGIRHRKAYQSRHTYACWSLSAGANPSFIASQMGHASAQMVFN
                     VYGAWMTDSNAEQIAMLNQKLADYVPMMSHGHQSDTRGLLKSVS"
     gene            1294..2064
                     /locus_tag="JN331_003471"
     CDS             1294..2064
                     /locus_tag="JN331_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY7261511.1"
                     /translation="MKISELAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPAEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2117..2512
                     /locus_tag="JN331_003472"
     CDS             2117..2512
                     /locus_tag="JN331_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261512.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2553..3296
                     /gene="cmoA"
                     /locus_tag="JN331_003473"
     CDS             2553..3296
                     /gene="cmoA"
                     /locus_tag="JN331_003473"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY7261513.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3293..4264
                     /gene="cmoB"
                     /locus_tag="JN331_003474"
     CDS             3293..4264
                     /gene="cmoB"
                     /locus_tag="JN331_003474"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY7261514.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKEQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4429..6858)
                     /locus_tag="JN331_003475"
     CDS             complement(4429..6858)
                     /locus_tag="JN331_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7261515.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNTL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     MYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEACNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFCADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYTELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKSAVQA"
     gene            complement(6883..7983)
                     /locus_tag="JN331_003476"
     CDS             complement(6883..7983)
                     /locus_tag="JN331_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY7261516.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1990 a         2068 c         1945 g         2164 t
ORIGIN      
        1 ggataaagtc aaatatccaa caggcgtcga aaaccacggt ggcacattac gcatctggtt
       61 taattttaaa ggtaaacgtg tcagggaaaa tcttggtgtc cctgacactg ccaagaacag
      121 gaagatcgcc ggagagctgc ggacatctgt atgttttgcc attcgtacag gaaattttga
      181 ttatgctgcg cagttccctg actctcctaa cctcaaggct tttggggtaa ataaaaaaga
      241 aattacggtg aaagaacttg aagaaaaatg gctggatctg aaacgaatgg aaatctctgc
      301 aaatgcattc aatcgctatg aatccattgc aaggatgatg gttccgaaga ttggtggcaa
      361 ccgactggta tcagcagtga ccaaagagga attgctgtat atcaggaaag atttgctgac
      421 cggataccaa aatccaacaa aaggtaaagc tccagtaaaa ggacgaagcg ttgtcacagt
      481 aaattattac atgacaacaa tcgctggaat gtttcagttt gctgcagatc acggttacat
      541 agaagcaaat cccttcgagg gaattaagcc tcttaaaaga gccagggcag aaccagatcc
      601 gctaactcgt gacgaattta ttcgcctgat agatgcttgc cggcatcagc agacaaaaaa
      661 cctgtggtca ttggctgtgt acacaggaat gcgtcatggt gaactggtct ccctggcctg
      721 ggaagatatc gatctgaaag caggaacaat tactatcagg cgcaattata cgaaactcgg
      781 tgagttcact ctacctaaaa ctgaagcaag tacaaacagg gttgtgcatc ttatccagcc
      841 cgctatcagt gtcctgaaaa atcaggctga aatgacaaga ctgggtaagc agcacaacat
      901 caaggttcaa ctacgtgaat atggacgttc agtaaatcat gaatgtactt tcgtatttaa
      961 cccccaggtg gttagaaaaa gcaaacaggt aggttttatc tacaaggtag attccattgg
     1021 cgactcatgg gaaacagcca ttaaacgtgc aggcatcagg catcgaaagg cataccagtc
     1081 acgacacact tatgcgtgct ggtcattatc tgccggagca aatccaagct tcattgccag
     1141 ccagatgggg catgcaagtg cccagatggt gttcaatgta tacggagcat ggatgactga
     1201 cagcaatgca gaacagatcg caatgctgaa tcagaagctg gcagattatg tcccaatgat
     1261 gtcccatggt caccaaagtg acacaagagg cttattaaaa tcagtgagtt agcttttaat
     1321 acccgtcatg ttaactgcgt ggagggcaac accacgcttt acgccctgcc gaaacccgag
     1381 gttgtcctgc gctggcgtga acagaccaca gatgacttcc gcttctgttt taagtttccg
     1441 gcgaccattt cgcatcaggc agcattacga cattgcgatg atttagtgac tgaatttttg
     1501 acccgcatgt caccgttggc tccgcgcatt gggcaatact ggctgcaact gcctgccaca
     1561 ttcggcccac gggagctgcc tgcgctttgg cattttctcg attctctccc tgccgaattt
     1621 aattatggtg tggaagtccg ccatccacag tttttcgcca aaggggaaga ggaacaaacg
     1681 cttaatcgcg gtttacatca gcgcggcgtt aatcgggtga ttttagacag ccgcccggtt
     1741 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
     1801 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
     1861 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
     1921 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
     1981 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
     2041 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
     2101 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
     2161 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
     2221 tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
     2281 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
     2341 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
     2401 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
     2461 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
     2521 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     2581 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtagctgaa gtcttcccgg
     2641 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     2701 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     2761 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     2821 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     2881 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     2941 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     3001 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     3061 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     3121 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     3181 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     3241 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     3301 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     3361 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     3421 caacgcggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     3481 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     3541 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     3601 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     3661 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     3721 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     3781 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     3841 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     3901 ttcgccgctg gagcatctct ggcagttgaa agaacaactg gtgaatgagg gtgaactggt
     3961 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     4021 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     4081 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     4141 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     4201 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     4261 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     4321 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     4381 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     4441 agcagactta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     4501 cagcgcagag ttaccggcac aggcatttgc cagctgcgag ctggggatat ccgccgttaa
     4561 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     4621 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     4681 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     4741 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgtata
     4801 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     4861 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     4921 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     4981 cgcatttttc accggatcgg cgcagaaatc accataacga acgtactgtt cgttcttctc
     5041 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     5101 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     5161 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     5221 ggcaaacacg tcaaaatcgt tacacgcttc aaattgcgga gcaacagcct gcttcatcgg
     5281 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     5341 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     5401 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     5461 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacattt gttctttacc
     5521 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     5581 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     5641 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     5701 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     5761 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     5821 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     5881 ttccgccgcc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     5941 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     6001 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     6061 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     6121 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     6181 gctccaggca atttttaagg tattcagcgt gttcattccc cacagcacca caacctggct
     6241 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     6301 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     6361 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     6421 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     6481 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     6541 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     6601 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     6661 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     6721 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     6781 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     6841 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     6901 atctttggcg ttgtactgga aataccgcgt taaaatgtcc agttcgtttt cgctcatgct
     6961 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     7021 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     7081 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     7141 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     7201 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     7261 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     7321 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     7381 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     7441 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     7501 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     7561 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     7621 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     7681 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     7741 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     7801 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     7861 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     7921 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     7981 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     8041 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     8101 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     8161 tttatca
//
LOCUS       DADLSB010000166         7814 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.166, whole genome shotgun sequence.
ACCESSION   DADLSB010000166 DADLSB010000000
VERSION     DADLSB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.166"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            179..397
                     /locus_tag="JN331_003477"
     CDS             179..397
                     /locus_tag="JN331_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY7261517.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            615..857
                     /locus_tag="JN331_003478"
     CDS             615..857
                     /locus_tag="JN331_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261518.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1187..2116)
                     /gene="fdhE"
                     /locus_tag="JN331_003479"
     CDS             complement(1187..2116)
                     /gene="fdhE"
                     /locus_tag="JN331_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY7261519.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2113..2748)
                     /gene="fdoI"
                     /locus_tag="JN331_003480"
     CDS             complement(2113..2748)
                     /gene="fdoI"
                     /locus_tag="JN331_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7261520.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTNAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2745..3647)
                     /gene="fdxH"
                     /locus_tag="JN331_003481"
     CDS             complement(2745..3647)
                     /gene="fdxH"
                     /locus_tag="JN331_003481"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7261521.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3660..6710)
                     /gene="fdnG"
                     /locus_tag="JN331_003482"
     CDS             complement(3660..6710)
                     /gene="fdnG"
                     /locus_tag="JN331_003482"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6123..6125),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7261522.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTETYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6904..7737
                     /gene="fdhD"
                     /locus_tag="JN331_003483"
     CDS             6904..7737
                     /gene="fdhD"
                     /locus_tag="JN331_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY7261523.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1808 a         2004 c         2077 g         1925 t
ORIGIN      
        1 tcctgggcga taactaaacc atttttgagt aataaatgaa acaactgtaa cgctcttatt
       61 atttctggaa aacgcctcgc aaaaacaaaa tgacatgcaa aaatccgctt tttttaaatg
      121 cttttccggc gtaattttcg cacatacatt aatgagtaat atatgtaagg tggtggtaat
      181 ggcgatgaat acggtttttc ttcatttatc agaggaggca attaaacggc tgaacaagct
      241 tcggggctgg cgtaaggttt cacgatctgc aattttacgc gaagcggttg agcaatatct
      301 ggagcggcag caatttccgg tgcgtaaagc aaagggaggc aggcaaaagg gggaggtagt
      361 tggtgttgat gatcagtgta aggagcataa ggaatgaaaa aaatttttca ttcttgaata
      421 taaaaaacag atgcccttat tctggtatta atacaaggct gttttacttg aacttataat
      481 aactgcaact gttacatcgt atctggaaaa cgcctcgcaa aaataaaaaa tgatgcgtaa
      541 atgagctttc tttaaagatt cggcaagggt acttttctct ccatagattc aaaaatgata
      601 ttggcgagat atttatgaac tctctggcag gtatagatat gggcagaatt ttactcgatt
      661 tatcgaatga ggtgattaag caacttgatg atctcgaggt gcagcgtaat cttcctcgtg
      721 cggacctatt aagggaagcg gtagatcaat acctgataaa tcaatcgcaa acagcaagaa
      781 ccagtgttcc tggcatctgg caagggtgtg aggaagatgg tgtcgaatat cagcgtaagc
      841 tgcgcgagga atggtaatgg gaaaaaatga tgtcaatcag attgctgaca acgtgcgcgt
      901 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaagtcatg caaattcaat
      961 atattgaagt ttattggtag gcctgataag cgcagcgcat caggcaatca tacgcttgtc
     1021 atcagtgaca tacccataac cttatcgatg tgttggaggg aagaacgagg caaacagaca
     1081 attgcggcat gatcttgaat gggtaacgcg gaatacgaga gatggtgtcg tcacacccta
     1141 tgagctgtaa tccgggcgac ctgcgccacc cggaaaacgt cagagattac tctccttcac
     1201 ccggaaacag gaacgggtta atggaactac gggcgtagcc ttcttgctcc attcgcgcgt
     1261 ccagtaccag agaggcgagg tcatctgcca cggcttcaac tttcggttct ttttcctgat
     1321 aaagaatctt caggtaagtg ccgcagtcat cgcagctttc ggctttaatc gcggcctgtt
     1381 cgtcatccag cgaccagtaa tgcagtttgc cgctctgctc gcagttgctg cattttacgc
     1441 gcactacgtg ccattcggtt tcacacaggt tgcagtgcag gtaacgcaga ccctgagtgg
     1501 tgccaatttg caccatgctg gacaccggca tagagccaca taccgggcaa tattgacgtt
     1561 gttcgccgta ttcagcgcgg gctttgccgg ggatcagatt ggccatctgc gcccagtaga
     1621 gcgacagcgc agcccagata aacggcgctt tatcgctgct gacggacgag aaatcagagg
     1681 caaacagcgc gctggccata tcttccagct cctgagtcga tgccttctcc agattctcaa
     1741 tcactgccag cgccgggccg ctcatttcag gtttcagctc agcaattagc gccatcagca
     1801 gcttttgcca gtgcttatca cgcggcagaa cgtgaatatc cagcgggggc ttgccctgtg
     1861 cgctggcttc tttaatgcgc gcggtcagat ccatctccag cggatggtcg tacagcacca
     1921 cttcctgggc gtgggcgata agcgcagcaa agcgcaggta atcacccagc ggattatttt
     1981 ctgccagctc gcgcagacgc tcggcgcggc ggttgtataa attcttgagc cgagggaaca
     2041 ataacggcgg aatcatatcc gccgtacgtt tctcgctcga acccagctca tcttgcggga
     2101 ttatgcgaat actcattcag ctttcttttc cgttgtcttg cggacctcac ggtaccagcg
     2161 cgggtgatgt ttcttcgccc aggcgttggt aacccatcct tccaccatgg cggtaatcgt
     2221 gcctttcacc caaagggcgg cgtagatatg caccatgata accacaatta acgccactgc
     2281 ggcaaatgaa tgcagcatta acgcgaatcg gatcaccggg attgagaaag caggcgcaaa
     2341 ataaggacgc cagataatca cgccgctcac cagcagcaga accaggaaaa taatcgccgc
     2401 ccagaaaacg catttctgac cgaagttata acgcccggtg tcacctactt cctcgttgac
     2461 gacgatctta cgaatattct tcgcccaaaa gatatcatcc cgattgatta ggttttgatg
     2521 ccagtaacgg aaaaacatga tgatgaacga ggcaaacata accacgccga caaacgggtg
     2581 cagaattcgc gccagctgcg gtgtgcccat gatttgcatc aaccagttga aggacgggaa
     2641 caaaaagccc agcccgctca ccgccgccag gatgaagcag aaggcggtga tccagtggtt
     2701 gatacgttcc ggcgcggtgt agcgcacgat ggtgtcacgt cgtttcattt gcgctcctcg
     2761 tctttctctt cgtgcagatt attctcttcc tcatccgcac ggttcggacc gacacccacg
     2821 tagtggaaga tactggccgc aaaggtagcc gcaaagccaa cagccgcgag cggtttccag
     2881 atgcctttcc agaatttcac ggtttcgctg atttccgggt tctccggcaa gccatgatac
     2941 agatttggct tgtcagcatg gtgcagcacg tacatgacgt gtgtaccacc gacgcctgcc
     3001 ggatcgtaca gacccgcatt gtcgtaaccg cgggttttca gctcagcaac gcgctcgctc
     3061 gccagcgttt tcatcgactc tttcgtaccg aagtgaatcg cgcccgttgg gcaggtcttc
     3121 acgcaggctg gttcttgccc aaccaccacg cggtcaacgc acagcgtaca tttgtagacg
     3181 cggttgtctt ccgggttgag gcgcggaatg tcgaacggac agcccgcaat gcaataaccg
     3241 cagccaatgc actgctcgga ctggaagtcg acgataccgt tggcatactg aataattgca
     3301 ccttccgccg ggcacgcttt caggcagcct ggatcggaac agtgcataca gccgtctttg
     3361 cggatcagcc attccagttt gtcgttctgc tccacttccg agaagcgcat taccgtccac
     3421 gatttggcgc ttaaatcatt ggggttgtcg tacaccccaa tgttattgcc gacggtatcg
     3481 cgaatgtcgt tccactctga acacgccacc tgacaggctt tacagccgat acaggtggta
     3541 acgtcgatga gtttcgccac ttcttcctgg aagtcccgcg cctgaggcgc gggggtcaga
     3601 ccgttagtcg cggaacgacg aatgatatct tgagattgat aagccatatg tcgtctccgt
     3661 tacacctttt ccacattcac aaggaaggac ttaaactccg gcgtctgcgt gttcgcatca
     3721 ccaacgaatg gcgttaaagt attggcaata aagccttttt tagctacgcc ttcatagccc
     3781 cagtgaatcg gaataccgat ggtatcgata tctttgccgt tcgctttcag cgtgcgaata
     3841 cgtttggtca ccaccgcttt ggctttgata tagccacggt tggaggagac tttcacggta
     3901 tcgccctggg caatgccaag tttattcgcc agcgactccc cgatttccac aaactgctct
     3961 ggctgcaaaa tcgcgttcaa cagcgcgtgt ttggtccagt agtggaagtg ctcggtcaga
     4021 cgataggtgg ttccgacata cgggaactta tcggctttac ccaatgcttc ggcgtcgtct
     4081 ttaaagatac gtgcagccgg gttcgagata acgtttggat gcagcgggtt agttcccagc
     4141 ggcgtttcaa acggctcgta gtgttccggg aacggacctt ccgccatctt atcgagggca
     4201 aacagacgcc ccatgccttc ctgctgcatg ataaacggcc cgacgccgct acccggaggc
     4261 gctgcgctgt agtccggaat atcccagccg gtccacttag tgccgtccca tttcagcaac
     4321 tgacgcttcg gatcccacgg gttaccctgc ggatctgcgg aggcgcggtt atacagaatg
     4381 cggcggttaa gcggccatgc ccatgcccag cccagcgtgt taccgaggcc agacggatcg
     4441 gcgttatcac gacgcgccat ctggttgcct tccggcgtcc agctaccggc gaaaatccag
     4501 cagccacagg aggttgtacc gtcatcgcgc aattgggcga acgagctaag ttgttggcct
     4561 ttcttgacga taaccgcacc ggttgccgga tcggtaatat cggccagcgc cttaccgttg
     4621 ctctccatcg ctacttcttc cgacttcggc tcatgcggaa tggcgtagtt ccaggtcatg
     4681 ttcagcacct gatccgggtt cgcgccaccc tgttcggcat acatcttgcg caagcgcagg
     4741 aagataccgg agaggatctc accgtcagtc agcgcaatcc ccggggcgtc cgcacctttc
     4801 cagtgccact gcaaccagcg acctgagtta acgattgaac cgttctcttc tgcgaagcag
     4861 gtcgatggca gacggaacac ttcggtctgg atcttcgacg agtcaacttc gttcagctca
     4921 ccgtggttct gccagaagtt agaggtttca gtgttcagtg ggtcgatggt gacgaggaac
     4981 ttcagtttcg acagacaacc gatcactttg tttttgttcg ggaatgaggc aacagggtta
     5041 aagccctggc agatatagcc attgactttg ccctctttca tcatctcgaa gtattgcagg
     5101 acgtcgtagc ctttatccca cttcggcaac cagtcaaagc cccagctatt ttccgccgtc
     5161 gctttatcac caaagaaggc cttcatcata gagacgaaga atttcgggta gttgccccag
     5221 tagtttacct ggccttccag cagcggtttt ggcgtgttgg cggtaaggta ggtttgcaga
     5281 tcggtctgct tctcgcttgg cagcgtcatg taacctggca gactctgcga cagtagcccc
     5341 aggtccgtca gcccctgaat attggagtga ccgcgcaggg cgttaacgcc gccgccggcc
     5401 atccccatat tgccgagcag cagctggatc atcgccatcg tacgaatgtt ttgcgcacca
     5461 acggagtgtt gcgtccagcc gagggcatac aggaacgagg cagttttatc gtgagcactg
     5521 gtttctgcga tgtattcgca gactttcagg aacgcgtctt ttggcgtacc acagatgttt
     5581 tcaaccacat ctggcgtgta gcgggaaacg tgctgtttca gcaagttcca cacgcagcgc
     5641 ggatgttgca gcgtggtatc gcgtttggcg aagccgtttt cgtccagttc ataagtccag
     5701 gaggatttat cgtacttgcg tttttccgcg tcgtagccgg tgaacaggcc atcttcaaag
     5761 ccgtaatcct cacgcacgat caggctggcg ttggtatagg tttcggtgta ttcgtggttg
     5821 aatttttcat tgttcagcag gtacagcaat acgcctgaca ggaaagcaat gtcagtaccg
     5881 gaacgaatag gggcatagta gtcagctacc gccgccgtac gcgtaaagcg aggatcgatc
     5941 acaatcagct tcgcgccgtt gtgaattttg gcttccatcg cccagcggaa cccgaccggg
     6001 tgagcttcag cggcgttacc gcccatcacc acgacgaggt tggcgttctt gatgtcgacc
     6061 cagtggttgg tcatcgcacc gcgaccaaat gttggagcaa gacttgctac cgttggtccg
     6121 tgtcagacac gcgcctggtt gtcgaccgcg agcataccca gcgcgcggga gaatttttgc
     6181 gttaaatagc cggtttcgtt actcgacgcg gaagcacaca gcatcccggt ggagagccag
     6241 cggttaacag tcacgccttc ggcgttttgc gcaatgtagt tagcatcgcg gtcttctttc
     6301 atcagtttgg cgatgcgatc aaacgcctct tcccaactga tttgctgcca tttatcagaa
     6361 cctggcgcac ggtattccgg aaacttcaga cggctttcgg agtggatgaa atccaccagg
     6421 ccagcgcctt tcggacaaag tgcaccgcgg ttgaccgggt gatccggatc gccttcgata
     6481 tggaagatag atgctttggc gttttttgct ccgtcaccga ggctgtacat caacagccca
     6541 cagcctacgg aacaataggt gcaggtatta cgggtttcgc gggtgcgcag cagtttatac
     6601 tgccgggttt ccgcgagtgc tacgctgggt gcaaaaccca gtgccgctgc cgtggtgcct
     6661 gccataccgc cagcgcagat cttaaagaac tgccttctgc tgacctgcat ggattgctcc
     6721 ttgtttcgac attgtcacgt cccatttaca ttcgcttgct gcgtgtgcag ggagtgggag
     6781 ttatttttct ttgcggaagg ggccgcaaag gtccagaatt ggctcaattt ccctccatct
     6841 aggaagggtt tgtaacagaa taccataatg ttggtgtgtg tgttcttatc tggttaagag
     6901 aaagtgaaaa aaacacagcg aaaagaaatc gaaaatgtga caaatatcac aggtgttcgt
     6961 caaattgagt tatggcggcg tgatgattta caacacccac ggttagatga ggtcgcggaa
     7021 gaagttcccg ttgcgctggt ctacaacggt atttcgcatg tggtgatgat ggcgtcgccc
     7081 aaagaccttg agtactttgc gctcggtttt tcgctttccg aggggataat agaaagtccg
     7141 cgcgatatct tcggcatgga tgtcgttcct tcctgtaatg gtcttgaagt acaaattgag
     7201 ctttccagcc gccgctttat ggggttgaag gagcgccgcc gggcgctggc gggacgtacg
     7261 ggctgtggcg tatgcggcgt ggagcaactt aatgacatcg gaaaaccggt gcagccgcta
     7321 ccgttcaccc agacgtttga tctcaacaaa ctggatgatg cattacgtca tctcaacgat
     7381 tttcagccag tggggcaact gactggttgt actcacgccg ctgcctggat gttgccatct
     7441 ggcgaactgg tcggcgggca tgaagacgtg ggtcgccatg tggcgctgga caaactgtta
     7501 ggccgccggt cacaagaagg ggaaagctgg cagcaaggtg cggtactggt ttccagtcgt
     7561 gccagttatg aaatggtgca aaagtcggcg atgtgcggcg tagagatttt gtttgcggtg
     7621 tctgccgcga ccacgcttgc tgtagaagtg gctgagcgct gtaatctgac actggtaggt
     7681 ttttgtaaac cgggtcgggc aacggtttat acccatccgc agcgtttgag caattaatta
     7741 aaataagtcc tgcgatatat attaaattgc aggacttatt catttcgtga attttattat
     7801 tttatttata aata
//
LOCUS       DADLSB010000167         7786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.167, whole genome shotgun sequence.
ACCESSION   DADLSB010000167 DADLSB010000000
VERSION     DADLSB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.167"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            111..644
                     /gene="ymdB"
                     /locus_tag="JN331_003484"
     CDS             111..644
                     /gene="ymdB"
                     /locus_tag="JN331_003484"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY7261524.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWCGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            586..2067
                     /locus_tag="JN331_003485"
     CDS             586..2067
                     /locus_tag="JN331_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY7261525.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2075..3232)
                     /gene="mdoC"
                     /locus_tag="JN331_003486"
     CDS             complement(2075..3232)
                     /gene="mdoC"
                     /locus_tag="JN331_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY7261526.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPRLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIDYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3626..5161
                     /gene="mdoG"
                     /locus_tag="JN331_003487"
     CDS             3626..5161
                     /gene="mdoG"
                     /locus_tag="JN331_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY7261527.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5154..7697
                     /gene="mdoH"
                     /locus_tag="JN331_003488"
     CDS             5154..7697
                     /gene="mdoH"
                     /locus_tag="JN331_003488"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY7261528.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPLVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1927 a         1857 c         2057 g         1945 t
ORIGIN      
        1 catcgaatat aactaagttt ctgaacaact cacgttgctg agcagaaaaa tgcgatttaa
       61 ccaaaaagcc tgctgtacac ttaagaaaca agaaggtgta aggaggcgtt atgaaaacgc
      121 gtattcatgt tgtgcagggt gatattacca aactggccgt cgatgtgatt gtgaatgcgg
      181 ctaatccgtc attaatggga ggcggcggcg tcgatggggc cattcatcgc gcagcgggtc
      241 cggccctgct ggatgcctgt ttaaaagtca ggcaacagca gggcgattgc cctacggggc
      301 atgccgttat tacgcttgca ggcgatcttc ccgctaaagc cgtagtacac accgtcgggc
      361 cagtctggtg tggtggtgaa caaaacgaag accagctttt gcaggatgcc tatctgaata
      421 gcctacgact ggtggcggca aacagctata cgtcagtggc ttttcctgca atcagtactg
      481 gggtttatgg ttaccctcgt gcggcagcgg ctgaaatcgc agttaaaacc gtttcagaat
      541 ttattacccg tcacgcttta cccgaacagg tatactttgt ctgttatgat gaagaaaacg
      601 cccacctcta cgaaagactc cttacccaac aaggagatga atgatttgcc ccggctggcg
      661 agcgcggtgc tgccactgtg ttcgcaacat cccggtcagt gtggtctttt ccctctggag
      721 aaaagtctgg atgcgtttgc cgcccggtat cgtctggccg aaatggcaga gcatacgctg
      781 gatgttcagt attacatctg gcaggacgat atgtcggggc ggttactgtt ttccgccctg
      841 ttagccgcag caaagcgtgg cgttcgcgtc cgtttgttgc tggacgacaa caatacgccc
      901 ggacttgacg acattttacg cttgcttgac agtcatccac gcattgaagt ccggcttttt
      961 aatcctttct cgtttcgctt gctacgtccg cttggatata tcaccgactt ttcccgtctc
     1021 aatcgccgga tgcacaataa aagtttcact gtcgatggcg tggttaccct agtgggagga
     1081 cgaaatattg gtgatgccta ttttggtgca ggggaagagc cacttttttc ggatttagat
     1141 gtcatggcaa taggccccgt ggtagaggac gttgccgatg atttcgcccg ctactggtat
     1201 tgcaaatcgg tttcaccctt acagcaggtg ctggatgtcc cggaaggtga aatggcggat
     1261 cgcatcgagt tacccgcctc ctggcataac gatgccatga cgcatcgtta tttacgcaaa
     1321 atggaatcca gtccatttat aaatcatctg gttgatggaa cgttgccgct tatctgggcg
     1381 aagacacgtt tattaagtga tgatccggcg aaaggggagg gcaaggcaaa acggcattca
     1441 ctgttaccgc agcgtctgtt cgatatcatg ggctcaccca gtgaacgcat cgatattatc
     1501 tcttcctatt ttgtaccgac acgcgcaggt gtggcgcaac tcttacggat ggtgagaaaa
     1561 ggggtaaaga ttgcgatcct aaccaattct cttgccgcta acgatgttgc tgtcgtccat
     1621 gccggatacg cgcgctggcg caaaaaattg ctccgctatg gcgtggaatt atatgaactc
     1681 aagccgacgc gtgaacaaag cagtacgtta cacgatcgcg gcataaccgg taattccggt
     1741 gccagcctgc atgctaaaac ttttagcatc gatggtaaaa cggtgtttat cggctctttc
     1801 aatttcgatc cgcgttcaac attgctcaat actgaaatgg gcttcgtgat agagagcgaa
     1861 acgctggcac agttaattga taaacgcttt attcagagcc agtacgatgc ggcctggcag
     1921 ctccgtctgg acaggtgggg acggatcaac tgggttgatc gtcatgcaaa gaaagaggtt
     1981 attctcaaaa aagaacccgc caccagtttc tggaagcggg ttatggtcag actggcgtcg
     2041 atattgcccg tggaatggtt attgtaaatg tggcttaacg ggctggtgct ttatcgttct
     2101 cacgcttaac aaccggttta ccagagaaca aaaacttcag taacgggatg cgtaaatgaa
     2161 tttcatacag aattatcgca atccctacta cgaatatcag gccacagaga aaaccaagcc
     2221 agttggaggt gatgtgcggt gtaatgtatg cgccgaaaaa cagcgttaac gggtggtgaa
     2281 ccagatagat aaacagcgat gcgttaacaa aataagtcac ccgcgctgac tggaagttaa
     2341 gcaaacggtg gccaaaggag aagaccacat tcaccatcca caggccgagg accatggtga
     2401 tcaccgactc ggtttcgtac atccaggcat cgccactgcc atagcgctgg ttgagtaaat
     2461 aagcgacaaa cgccaatgct gcagcgaggg tacagccacg agacggcgtg gtaaacaagg
     2521 ctttaagatg agggaaaatg aaagccagtg cgccgaggat aaagaacggt aaataaaata
     2581 gcgtttgcat gacaataaaa ttgaacatgc cattactcag aatgggtgga tacacaataa
     2641 aaatcgttct tcttattacc gcatagtcga tgccgaggca taaaaaaatc accgatagtt
     2701 ttaccatcga gaatttttta ttcgttttat cagaattttc taaattattt ctgatgcgct
     2761 taaatatcca tacacacagc gtcgtcatga ctactaacac cagtaaaaac cacaggtgtg
     2821 atattaattc ccaggccaac gtattatatt tgtcatacaa tgatagccta ggccaacttt
     2881 ccgctttccc tttgacatat tgcagcataa taaattgcgg taatgtcagt agggggatgg
     2941 ctgttaacat cgggatacct acacgttcga cacgtacttt ccaccatttt ttcaagggat
     3001 agcgtaaaaa aagcatgtag gaaaagtatc cggatataac gaaaaatacc tgcatgcgga
     3061 acgagtggat gaagtcatta aaaagggtca gccacaatga cggttcggcg ctattcacat
     3121 gccatgtatg gctcgaatag attaaagaaa tatgaaaagg gatccctaac aacatcagcc
     3181 aggcgcggat ggagtcgagg aaatattcac gttgcgcggg tactggattc atatatggtt
     3241 aactaatctc ggatttttcg tcttatccct gtcgggttat gcctttaggc ttgttgccat
     3301 agcgacaccg acctgaccgc gccaggcgca ggcttcaagg tttttatgca tagcatcatc
     3361 gctaccacta accagaatgg aagcgtctgt aagacggttg ataaataaat ttgctggcaa
     3421 accctacacg aagtcgatgc ttctgtcttt aggagaagca cggaaagtga aaacggttgc
     3481 aatcaggtgc ttaatccatg agccagcgtg ctgaacgata ccgggattca gttgtcggaa
     3541 tggctggtta tccattaaaa tagatcggat cgatataagc acacaaaggg ggaagtgctt
     3601 actaattatg aaacataaac tacaaatgat gaaaatgcgt tggttgagtg ctgcagtaat
     3661 gttaaccctg tatacatctt caagctgggc tttcagtatt gatgatgtcg caaagcaagc
     3721 tcaatcctta gccgggaaag gctatgaggc gcctaaaagc aacttgccct ccgttttccg
     3781 cgacatgaaa tacgcggact atcagcagat ccagtttaat catgacaaag cgtactggaa
     3841 caatctgaag accccattca aactcgagtt ctaccatcag ggtatgtact tcgatacccc
     3901 ggtcaaaata aatgaagtga ctgccaccgc agtcaaacga atcaaataca gcccggatta
     3961 tttcactttc ggcgatgttc agcatgacaa agacacggta aaagaccttg gttttgccgg
     4021 ttttaaagtg ctttacccga tcaacagcaa agataaaaac gatgaaatcg tcagcatgct
     4081 cggggccagc tatttccgcg tgattggtgc aggtcaggtt tatggccttt ctgcccgcgg
     4141 cctagcaatt gataccgctt tgccatcggg tgaagaattt ccacgcttca aagagttctg
     4201 gatcgagcgt ccaaaaccga ctgataaacg tttaaccatt tatgcattgc ttgactcgcc
     4261 gcgtgcgaca ggtgcttaca aattcgtggt tatgccaggg cgtgacacgg ttgtggatgt
     4321 gcagtcgaaa atctatctgc gcgataaagt cggcaaactg ggggttgcac cgttaaccag
     4381 tatgttcctg tttgggccga accaaccgtc tcctgcaaat aactatcgtc cggagttgca
     4441 cgactctaac ggtctgtcta tccatgctgg taatggcgaa tggatctggc gtccgttgaa
     4501 taacccgaaa catttagcgg tcagcagctt ctccatggaa aacccgcaag gctttggtct
     4561 gttgcagcga ggtcgtgatt tctcccgctt tgaagatctc gatgatcgtt acgatctccg
     4621 tccaagcgca tgggtgactc cgaaagggga gtggggcaaa ggcagcgttg agctggtgga
     4681 aattccaacc aacgatgaaa ccaacgataa catcgtcgct tactggacgc cggatcagct
     4741 gccggagccg ggtaaagaga tgaactttaa atacaccatc accttcagcc gtgatgaaga
     4801 caaactgcat gcgccagata acgcatgggt gcaacaaacg cgtcgttcaa cgggggatgt
     4861 gaagcagtcg aacctgattc gccagcctga cggtactatc gcctttgtgg tcgattttac
     4921 cggtgcagag atgaaaaaac tgccagagga taccccggtc acagcgcaaa ccagcattgg
     4981 tgataatggt gagatagttg aaagcacggt gcgctataac ccggttacca aaggctggcg
     5041 tctggtgatg cgtgtgaaag tgaaagatgc caagaaaacc actgaaatgc gtgctgcgct
     5101 ggtgaatgcc gatcagacgt tgagtgaaac ctggagctac cagttacctg ccaatgaata
     5161 agacaactga gtacattgac gcaatgccca tcgccgcaag cgagaaagca gcattgccga
     5221 agactgatat ccgcgccgtt catcaggcgc tggatgccga acaccgcacc tgggcgcggg
     5281 aggatgattc cccgcaaggc tcggtaaagg cgcgtctgga acaagcctgg ccagattcac
     5341 ttgctgatgg acagttaatt aaagacgacg aagggcgcga tcagcttaag gcgatgccag
     5401 aagcaaaacg ctcctcgatg tttcccgacc cgtggcgtac caacccggta ggccgtttct
     5461 gggatcgcct gcgtggacgc gatgtcacgc cgcgctatct ggctcgtttg accaaagaag
     5521 agcaggagag cgagcaaaag tggcgtaccg ttggtaccat ccgccgttac attctgttga
     5581 tcctgacgct cgcgcaaact gttgtcgcga cctggtatat gaagaccatt cttccttatc
     5641 agggttgggc gctgattaat cctatggata tggttggtca ggatttgtgg gtttccttta
     5701 tgcagcttct gccttatatg ctgcaaaccg gtatcctgat cctttttgcg gtactgttct
     5761 gttgggtgtc cgccggattc tggacggcgt tgatgggctt cctgcaactg cttattggtc
     5821 gcgataaata cagtatatct gcgtcaacag ttggcgatga accattaaac ccggagcatc
     5881 gcacggcgtt gatcatgcct atctgtaacg aagacgtgaa ccgtgttttt gctggcttgc
     5941 gtgcaacgtg ggaatcagta aaagccaccg ggaatgccaa acactttgat gtctacattc
     6001 tgagtgacag ttataacccg gatatctgcg tcgcagagca aaaagcctgg atggagctta
     6061 tcgctgaagt cggtggcgaa ggtcagattt tctatcgccg ccgccgtcgc cgcgtgaagc
     6121 gtaaaagcgg taatatcgat gacttctgcc gtcgctgggg cagccagtac agctacatgg
     6181 tggtgctgga tgctgactcg gtaatgaccg gtgattgttt atgcggcctg gtgcgcttga
     6241 tggaagccaa cccgaacgcc gggatcattc agtcgtcgcc gaaagcgtcc ggtatggata
     6301 cgctgtatgc gcgctgtcag cagttcgcga cccgcgtgta tgggccactg tttacagccg
     6361 gtttgcactt ctggcaactt ggcgagtcgc actactgggg acataacgcg attatccgcg
     6421 tgaaaccatt tatcgagcac tgcgcactgg ctccgctgcc gggcgaaggt tcctttgccg
     6481 gttcaatcct gtcacatgac ttcgtagaag cggcgttgat gcgccgtgca ggttgggggg
     6541 tctggattgc ttacgatctc ccgggttctt atgaagaatt gccgcctaac ttgcttgatg
     6601 agctaaaacg tgaccgccgc tggtgccacg gtaacctgat gaacttccgt ctgttcctgg
     6661 ttaagggtat gcacccggtt caccgtgcgg tgttcctgac gggcgtgatg tcttatctct
     6721 ccgctccgct gtggtttatg ttcctcgcgc tctctactgc attgcaggta gtgcatgcgt
     6781 tgaccgaacc gcaatacttc ctgcaaccac ggcagttgtt cccggtgtgg ccgcagtggc
     6841 gtcctgagct ggcgattgca ctttttgctt cgaccatggt gctgttgttc ctgccgaagt
     6901 tattgagcat tttgcttatc tggtgcaaag gaacgaaaga atacggcggc ttctggcgcg
     6961 ttacattatc gttgctgctg gaagtgctgt tttccgtgct gctggctccg gtacgcatgc
     7021 tgttccatac ggtcttcgtt gtcagcgcgt tccttggctg ggaagtggtg tggaattcac
     7081 cgcagcgtga tgatgactcc acttcatggg gcgaagcgtt caaacgccac ggctcacagc
     7141 tgctgttggg gttagtgtgg gctgttggga tggcgtggct ggatctgcgt ttcctgttct
     7201 ggctggcacc gattgtcttc tcgttgatcc tgtcaccgtt tgtttcggtg atttccagcc
     7261 gtgccaccgt tggtctgcgc accaaacgct ggaaactgtt cctgatcccg gaagagtatt
     7321 caccgccgct ggtgctggtt gataccgatc ggttccttga gatgaatcgc caacgctccc
     7381 ttgacgatgg ctttatgcac gcagtgttta acccgtcatt taacgctctg gcaaccgcaa
     7441 tggcgacagc gcgtcaccgc gccagtaagg tgctggaaat cgcccgtgac cgccacgttg
     7501 aacaggcgct taacgagacg ccagagaagc tgaatcgcga tcgtcgcctg gtgctgctaa
     7561 gcgatccggt gacgatggcc cgtctgcatt tccgcgtctg gaattcccct gaaagatatt
     7621 cttcatgggt gagttattac gaagggataa agctcaatcc actggcattg cgtaaaccgg
     7681 atgcggcttc gcaataaaaa cgtagttgcc tgatgcgcta cgcttatcag gcctacatcg
     7741 ttcctgcaat ttattgattt tgcaagattt tgtaggtcgg ataagg
//
LOCUS       DADLSB010000168         7774 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.168, whole genome shotgun sequence.
ACCESSION   DADLSB010000168 DADLSB010000000
VERSION     DADLSB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.168"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(108..1361)
                     /locus_tag="JN331_003489"
     CDS             complement(108..1361)
                     /locus_tag="JN331_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7261529.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTHYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1468..2388
                     /locus_tag="JN331_003490"
     CDS             1468..2388
                     /locus_tag="JN331_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7261530.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNCSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAELAELAELAKEPFVFFDPHVGTGLYDDI
                     LGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDA
                     VSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            2523..3743
                     /gene="mlc"
                     /locus_tag="JN331_003491"
                     /gene_synonym="dgsA"
     CDS             2523..3743
                     /gene="mlc"
                     /locus_tag="JN331_003491"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY7261531.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3868..4563
                     /gene="bioD"
                     /locus_tag="JN331_003492"
     CDS             3868..4563
                     /gene="bioD"
                     /locus_tag="JN331_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7261532.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KDTPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4516..5808)
                     /gene="clcB"
                     /locus_tag="JN331_003493"
     CDS             complement(4516..5808)
                     /gene="clcB"
                     /locus_tag="JN331_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY7261533.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(5967..6581)
                     /gene="dmsD"
                     /locus_tag="JN331_003494"
     CDS             complement(5967..6581)
                     /gene="dmsD"
                     /locus_tag="JN331_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY7261534.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6624..7478)
                     /locus_tag="JN331_003495"
     CDS             complement(6624..7478)
                     /locus_tag="JN331_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY7261535.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7480..>7774)
                     /locus_tag="JN331_003496"
     CDS             complement(7480..>7774)
                     /locus_tag="JN331_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261536.1"
                     /translation="CPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEF
                     GPIEELRQKHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
BASE COUNT         2048 a         1988 c         2043 g         1695 t
ORIGIN      
        1 cgctgcggga attggctggc gcttcgtcta ttttaggatg gctattaacg ttggtttgag
       61 caggcaataa gtccggacgg gtatttaccg cagtccggac ttatttttca ggcgtgcaga
      121 cgacgatgca aacgcgtccc gaccagcaga gcaatgacca gcatcagagc aataaatgcg
      181 ccgacgccgt tccagccata gttatgccag aaaacaccac ccagcgtccc ggcaatactc
      241 gaccccagat agtaactgaa cagatacagc gaggaggcct ggcctttagc gcgttttgca
      301 cgggggccga tccagctgct ggctactgag tgggctgcga agaatccggc tgagaagagt
      361 aacattccgg caaagatcag ccacagcgag ctaaataagg tcatcagtaa accaaacagc
      421 ataactcccg tcgaaaacaa catcaccgga ccgcgcccat agtgggtggt catggttccg
      481 gctttgggtg agctccatgt accggtcaaa taagccagcg ataataagcc aaccacggcc
      541 tgactgacat gccaggggga aagcatcaac cgatagccga tgtaattaaa cagcgtgacg
      601 aacgacccca tcagcaaaaa gccttctgcg aacaataacg gtaatccccg gtcacgccag
      661 tgcagacgaa agttgataaa taacgtctta gggcgcagcg aagttgggca aaaatggcgt
      721 gattcaggga ggattttcca gaacatcaac gccgaggcca gcgcgaaaca accgattgcc
      781 gccagagcaa ttcgccagtt gaaaaagtcc gtgaagacac cactaattaa gcgtccgctc
      841 atgccgccaa ttgagttgcc gctgatatac aaccccatcg aaaaggccac gaaactgggg
      901 tggatttcct cgctaagata agtcatgcca acagctgcca cgccacttaa cgaaagccca
      961 atcaaggcgc gcataatcaa aatgccgtgc cagctggtca tcattgtcga aagtaacgta
     1021 caaatggagg ccaacagtag cgccgtgacc atcactggtt tgcgaccaat ggcatcggat
     1081 agcgggccag taaatagcaa accgatagcc aacatcgccg tggaaatgga cagtgaaata
     1141 ctactgttcg cgggcgttaa gccaaactcc tgcgaaagca ccggaaggat aggctgcaca
     1201 caatagagaa gtgcaaaggt tgccagtccg gcagagaaca gcgccagggt gacgcgcata
     1261 aattgcggcg taccgcgttt aataaattga tttggctgag aaatgctttg cttgtcagtg
     1321 tcgcttgccg gagcgccatc aacagttgta gtacggctca cttgaaatcc ttgctaaata
     1381 tgcctgtgga tcaggcttat acatagggta ggaaaatcga attgttctgt ctaatatatt
     1441 aataatctca aataagatgt tttaaatatg aatattgaac ttcgtcatct gcgttacttt
     1501 gttgctgttg cggaagagct gcattttggg cgcgcggctg cccgcctgaa tatttcgcaa
     1561 ccgccgctaa gtcagcagat tcaggcactg gagcaacaaa tcggtgcccg actgctggca
     1621 cgaactaatt gcagtgtatt gctgacggca gcaggaaaac agtttcttgc agatagtcgg
     1681 caaatcctgt ctatggtgga tgacgctgcc gctcgcgctg aaagactgca tcagggtgaa
     1741 gcgggggagt tgcgtattgg ttttacttcg tccgctcctt ttattcgggc ggtgtccgat
     1801 acgttatcgc tgtttcgccg tgattatcct gatgtccatt tacaaacccg cgaaatgaac
     1861 actcgcgagc aaatcgctcc gctcattgaa ggaacgctgg atatgggatt gctgcgtaac
     1921 acagcgttac cggagacgct tgaacacgca gtcatcgtcc atgaaccgct tatggcgatg
     1981 atcccgcacg atcatcccct ggcaaataat ccgagtgtaa cactggctga actggctgaa
     2041 ctggctgaac tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg
     2101 actgggctgt atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc
     2161 actcaggagg tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt
     2221 tcaattttgc ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt
     2281 gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt
     2341 ccagctgcgc gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag
     2401 cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt
     2461 attgaagtgc ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg
     2521 cggtggttgc tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg
     2581 cggtttatcg cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg
     2641 cgcagctggc tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg
     2701 tgcgagagct ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg
     2761 ttgaaactga agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg
     2821 ctctgcgcga tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag
     2881 atgacttgcc attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc
     2941 agaaaaaact tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg
     3001 aaaatggtat tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg
     3061 aggcgctgga gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga
     3121 cgatggcaga ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta
     3181 tcgatcacaa cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca
     3241 gtagtctcgt ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg
     3301 ggaaccacgg ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc
     3361 tgcgtctcaa tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat
     3421 tgtgtcaggc ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg
     3481 cgcatgtagg gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga
     3541 ttggctcacc gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc
     3601 gtcagcaggc ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta
     3661 accagggcac gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt
     3721 tgattcgtct gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc
     3781 aatttgggcc aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat
     3841 ttgtgaagca taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac
     3901 acttctgtag ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga
     3961 aaaacggttg cgggatataa acccgtagcg aaggggagca aagatacacc cgaagggctg
     4021 cgtaataaag atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca
     4081 gttaatccta tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat
     4141 tacaccctca tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta
     4201 gaagggactg gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta
     4261 gtgcaggaac aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat
     4321 gcactgctaa cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct
     4381 aaccgaatca acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa
     4441 cttccggcac cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg
     4501 gggcaataca tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg
     4561 taacgtccgc gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg
     4621 atactccccg gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc
     4681 cgccaacagt gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg
     4741 gaaccataat cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt
     4801 cggtgtaaag acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt
     4861 acaaaggaag atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac
     4921 ggtgctatag ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa
     4981 cccgcctagt gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca
     5041 ggcgttcatt aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat
     5101 aatcagcgca tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc
     5161 gtcgctatga ttaatcagat tgctaaccag caatgccacg acggcggaaa taatcaccgg
     5221 gccgagagag gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc
     5281 aagcggggca cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt
     5341 ccattcctgg cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag
     5401 aatcatcgca ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag
     5461 cgatttaacc aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta
     5521 atcggtcggc gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc
     5581 cgccgccagt ccgccgagcg ccggagttag cagccgtcgc cagggggaaa ggtttgtcgc
     5641 tgcattgacc agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc
     5701 atgacgaaac ccggcaacgg caaagaccgc gagaataccg acgactgttg cgataagcag
     5761 acggcggaac atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg
     5821 gcaatgaaac acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg
     5881 ctttaagaga taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt
     5941 caccatccgg caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc
     6001 aacagttgtg attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag
     6061 aaagggtgtt ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg
     6121 tgccatgcca gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc
     6181 atcaacagca gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg
     6241 cctttctcgc gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg
     6301 cgatccagcc agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa
     6361 cgctgccagg cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc
     6421 gctaatgacg cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca
     6481 accagaggtg cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc
     6541 gccgcgacag aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt
     6601 gcgggttgcc ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg
     6661 ccataaaaga gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca
     6721 agtccggcaa cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct
     6781 gcgcataaca ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg
     6841 gcttgttgca cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg
     6901 gcacaagcga tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg
     6961 cgggctgccc gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag
     7021 aaagccagcg tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag
     7081 gtcatcatcc agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc
     7141 aacgcttgcg gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa
     7201 aatatggaac cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag
     7261 ttaaacgctc gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc
     7321 aacagccaga ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg
     7381 gcgaaccagc caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta
     7441 aagataacca gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc
     7501 cagataacca gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt
     7561 gggttttgtg aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg
     7621 gcgcagctct tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat
     7681 gggttgtttc ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt
     7741 ttcagcattg tactgtggtg cgccgtacgg acaa
//
LOCUS       DADLSB010000169         7735 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.169, whole genome shotgun sequence.
ACCESSION   DADLSB010000169 DADLSB010000000
VERSION     DADLSB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.169"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..518
                     /locus_tag="JN331_003497"
     CDS             <1..518
                     /locus_tag="JN331_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261537.1"
                     /translation="IIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMEN
                     NHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYI
                     STLGAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHKHYKTVETYYAAINVISQSL
                     SFSADEIKTYL"
     gene            690..797
                     /locus_tag="JN331_003498"
     CDS             690..797
                     /locus_tag="JN331_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261538.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(850..1608)
                     /locus_tag="JN331_003499"
     CDS             complement(850..1608)
                     /locus_tag="JN331_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY7261539.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1895..2824
                     /gene="pfkB"
                     /locus_tag="JN331_003500"
     CDS             1895..2824
                     /gene="pfkB"
                     /locus_tag="JN331_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY7261540.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2925..3215
                     /gene="ghoS"
                     /locus_tag="JN331_003501"
     CDS             2925..3215
                     /gene="ghoS"
                     /locus_tag="JN331_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY7261541.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3321..4181
                     /locus_tag="JN331_003502"
     CDS             3321..4181
                     /locus_tag="JN331_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY7261542.1"
                     /translation="MWQAIIRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4222..4758)
                     /locus_tag="JN331_003503"
     CDS             complement(4222..4758)
                     /locus_tag="JN331_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261543.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4905..5573
                     /gene="hxpB"
                     /locus_tag="JN331_003504"
     CDS             4905..5573
                     /gene="hxpB"
                     /locus_tag="JN331_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAY7261544.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     EQPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5736..6326
                     /locus_tag="JN331_003505"
     CDS             5736..6326
                     /locus_tag="JN331_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7261545.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6459..>7735
                     /locus_tag="JN331_003506"
     CDS             6459..>7735
                     /locus_tag="JN331_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAY7261546.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     DSFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRT"
BASE COUNT         1992 a         1862 c         1870 g         2011 t
ORIGIN      
        1 aaataatcga tatctttctg aatgcattaa ccactccaat agcacaagag cttttaaaca
       61 aaaaactggt gatgagtatt ttagccatga aaatacatga tggtgagcca ggattatacg
      121 ccgcaatgga aaataatcac cctttgtgtg tcacacggtt cctctctaaa attaatggca
      181 tcgcctttaa atacaagttg agcaaagcta acatcatgga tttattaaaa ggagctacag
      241 cacagggaac ccctgcatta tacatcgcca tgagcaaggg taatgaagac gtcgtgttat
      301 cttatatatc gacgctgggt gcttttgcaa aaaaacattc ttttagtcaa catcagttat
      361 ttacactatt ggccgctaaa aatcatgaca acatgtcagc tgttcatata gccattcatc
      421 ataagcatta taaaactgta gaaacatatt atgctgctat aaatgtcatc agccaaagcc
      481 tgagttttag tgctgatgaa ataaagacgt atttataacc agctatttac gatatatatc
      541 gatacataat tttatttttt tcataaaacg cagaaacagt gaccatgtgc acaacgccac
      601 ccgacggctg taataacaaa aaatcccttg atgcctgtcc cttttgttac actccgttat
      661 cacgcacaag agatatgcag gacactggta tgccgactaa acgctttgat aaaaaacact
      721 ggaagatggt ggtggtgcta ctggcaatct gtggcgctat gttgttgcta cgttgggcag
      781 caatgatttg gggctgagca gtgacaaacg aagacaacca gaccgcataa cacggtccgg
      841 cagataattt tacatgctgt agcccagcga taaggttgtc cgacggtcgg tatgttccgg
      901 tgcagactct ggaggttcag agttccatgt gacgttgtaa gccaccttca gtccaaaatg
      961 ttcattaata gcaacattta atgcgctttc ggagttcagc gttgtgtctt ccgcgccaaa
     1021 gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac tgccatgcat aagccccgga
     1081 ggcatagccc agcggctggg tttcactggc attgtcggta tatttgtcgt aacgcacacc
     1141 tggaccgaat tcaaagcgga aactgtgtac cgggccattc aaaaactgac gaccataacc
     1201 cgcggtcaac acatcgcgct ctcgatagcc gttataacgg tctgtcagcc agcttgcctg
     1261 accaaataaa tagtcataat cagttaaatt aaagcggcta cgcccgcccg ccgcatattt
     1321 ttctgaagaa cgctcatcat tagaagaagt attactggcg ttcccccaca gcgaccaggc
     1381 agtggtttgt ccataccagg tcatggtggt gtcagccgta agtgaggagc ttttcgtatt
     1441 gcctgattgt gcaagatatc ctgcgttcag attaccttcg aaaggttttt tagcgctggc
     1501 aggatcgtcc atgacagtaa aaacggaatc atcggcagct gcattcagtg acgcaaacat
     1561 gccccccgcc aacataacga tggcaggaac tgtcttcaaa agcttcattt atcaagagtc
     1621 cgtacaacaa aaaaagagac catcgcggtc ccggaaactt tcttaaggat caaagattag
     1681 cgtccctgga aaggtaacga attataaaaa ggcgcgaata acttagcaat gtattcttat
     1741 ttcatttttt gaataagcat gtggcgaaaa cagattttta tttatatata tttatctgca
     1801 aaattttaaa taaagctcta ataaatcata ttgttaattt cttcactttc cgctgattcg
     1861 gtgccagact gaaatcagcc tataggagga aatgatggta cgtatctata cgttgacact
     1921 tgcgccctct ctcgatagcg caacaattac cccgcaaatt tatcccgaag gaaagctgcg
     1981 ctgtaccgca ccggtgttcg aacccggggg cggcggcatc aacgtcgccc gcgccattgc
     2041 ccatcttgga ggcagtgcca cagcgatctt cccggcgggt ggcgcgaccg gcgaacacct
     2101 ggtttcactg ttggcggatg aaaatgtccc cgtcgctact gtagaagcca aagactggac
     2161 ccggcagaat ttacacgtac atgtggaagc aagcggtgaa cagtatcgtt ttgttatgcc
     2221 aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa gagcaagttc tggaaattga
     2281 atccggggcc atcctggtca taagcggaag cctgccgcca ggtgtgaagc tggaaaaatt
     2341 aacccaactg atttccgctg cgcaaaacca agggatccgc tgcatcatcg acagttctgg
     2401 cgaagcgtta agtgcagcac tggcgattgg taacatcgag ttggttaagc ctaaccaaaa
     2461 agaactcagt gcgctggtga atcgcgaact cacccagcca gacgatgtcc gcaaagccgc
     2521 gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc gtttcccttg gtccacaagg
     2581 agcgctgggt gttgatagtg aaaactgcat tcaggtggtg ccaccaccgg tgaaaagcca
     2641 gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg acactgaaac tggcagaaaa
     2701 tgcctctctt gaagagatgg tccgttttgg cgtagctgcg gggagtgcag ccacactcaa
     2761 tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa atttacgctt acctttcccg
     2821 ctaacaaaaa cattccccca gcattggggg aatcaccacc aacctgtcgg caacgcgttt
     2881 ctccgactat gctcaaaagt catgtgataa caaaggggtg aactatggcc agtggcgatc
     2941 ttgtccgtta tgtcataacc gtcatgttgc atgaggatac attgactgaa attaacgagt
     3001 tgaataatta cctgactcgc gacggttttt tgctcaccat gacggatgat gagggaaata
     3061 tccatgagct ggggactaac acttttggac ttatcagtac ccaaagtgaa gaagaaatta
     3121 gagaactggt ttcggggctt acccaaagtg caaccggcaa agatcctgaa atcaccatca
     3181 cgacctggga ggaatggaat agcaacagaa aataaatggt ttttgggcaa taatcagtct
     3241 gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc gctaacctta tgatctggca
     3301 gacaacatgg gagagacatc atgtggcagg caatcattcg tcttttgagc gagcagttag
     3361 gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg agaagtccat gccgcatggc
     3421 atttgcgcta tgcaggacat gactttttcg tcaaatgtga tgaaagggaa ctgcttcccg
     3481 gttttaccgc cgaagccgac caactggagt tactgtcgcg tagtaaaacc gtcaccgtgc
     3541 ctaaggtttg ggcagtaggc gctgaccgtg actacagttt tctggtgatg gattatctcc
     3601 cacctcgtcc gctggatgcg catagcgcat ttattcttgg tcagcaaatt gcgcgtttac
     3661 atcaatggag tgaccaacca caatttggcc tcgattttga taactcgctc tccacaactc
     3721 cacagcccaa cacctggcaa cgtcgctggt caacgttttt tgctgaacaa cggattggct
     3781 ggcagttgga actggcagca gagaaaggga tcgctttcgg caatatcgac gccatcgtcg
     3841 agcatattca gcagcgtctg gcctcgcatc aaccgcagcc ttctctgttg cacggcgatt
     3901 tatggtccgg caactgtgcg ctgggtccgg atggcccgta cattttcgac cctgcctgct
     3961 actggggtga ccgagagtgc gacctagcga tgttaccgct gcatactgaa cagccgccac
     4021 aaatctatga cggctatcag tcagtatccc cgctacctgc cgatttcctt gaacgtcaac
     4081 cggtttacca actctacacg ctgttaaatc gtgcaagatt atttggcggt cagcatttgg
     4141 ttattgctca gcagtcattg gatagattat tagcagcatg atatgggttg aggataatgg
     4201 ccgctccgtg cggccttttg attaaataaa cccaagcaga gaaaagaaca cataacctgc
     4261 agcaatacaa ataaccggca ggatatatag tgagaaaaac tgcaggaaaa tagtatggtg
     4321 cggaacaaca atacgggact caatttgttc acgcgttagc ccctcttccc ctttggcttt
     4381 ttccagaatg agttgatctt caacaccttc ccgcaagaag cgcgcctggc gactcatccg
     4441 ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg atgaaaatca cccagaacat
     4501 aacgttcaca ccaccattaa aattaggggt cggggagtta taccagaaca gattcaaaaa
     4561 aggcgtattc gcctgcatca tatcgatcat gacatgagca aagtcgagca tgaccgcatt
     4621 aatgccttcc tggttttcct gccgggtatt cataaatttc agcaaagaaa tcaatgtaga
     4681 gatcaatgca ggtataaaaa tcacccaacc caaaatcctt ttcaaaacag caatgcgtcc
     4741 agcttgttga tacgtcatga gttctccttg attaagacgc gtcgtttcac ttagtttacc
     4801 tgtagatatc tgttttcgcc cattctttaa aggcgatatg ataggcgctt aatcataagc
     4861 acggcttaat accttacaca taatgctcta aaggagaggt tgtaatgtca accccgcgtc
     4921 agattcttgc tgcaattttt gatatggatg gattacttat cgactcagaa cctttatggg
     4981 atcgagccga actggatgtg atggcaagcc tgggggtgga tatctcccgt cgtaacgagc
     5041 agccggacac cttaggttta cgcatcgata tggtggtcga tctttggtac gcccggcaac
     5101 cgtggaatgg gccaagccgt caggaagtag tagaacgggt tattgcccgt gccatttcac
     5161 tggttgaaga gacacgtcca ttattaccag gcgtgcgcga agctgttgcg ttatgcaaag
     5221 aacaaggttt attggtggga ctggcctccg cgtcaccact acatatgctg gaaaaagtgt
     5281 tgaccatgtt tgacttacgc gacagtttcg atgccctcgc ctcggctgaa aaactgcctt
     5341 acagcaagcc gcatccgcaa gtatatctcg actgcgcagc aaaactgggc gttgacccac
     5401 tgacctgcgt ggcgctggaa gactcggtaa atggcatgat cgcttctaaa gcagcccgca
     5461 tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga tccacgtttt gtattagcaa
     5521 acgtcaaact ctcatcgctg acagaactca ccgcaaaaga ccttctcggt taatgaccag
     5581 gggcagtgat cacgctgccc ctggttcttt atctgaattt gcattcaact gacggattaa
     5641 tcgtcaattt aagagaaaga gttacaccgt caccacttcc gtgcactgta taaaaatcct
     5701 atactgtacg tatcgacagt ttagtgagtt ttatcatgac ggcggaaggt caccttctct
     5761 tttctattgc ttgtgcggta tttgccaaaa atgccgagct gacgcccgtg ctggcacagg
     5821 gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg tttgttaccg gacatcgatc
     5881 acccaaagtc gtttcttggg cagcgattaa aatggatatc aaaaccgatc gcccgcgctt
     5941 ttgggcatcg tggttttacc cacagtctgc tggcggtatt tgcgctgctg gcaacctttt
     6001 accttaaggt tccggaaggc tggttcattc cggctgatgc gctacaagga atggtgctgg
     6061 gttatttgag ccacatactt gccgatatgc tgacacccgc cggtgttccc ctgctctggc
     6121 catgccgctg gcgtttccgc ttgcctatcc tggttcccca aaagggcaac caactggaac
     6181 gttttatctg catggcatta tttgtctggt cggtatggat gccccattca ttacccgaga
     6241 acagcgctgt tcgttggtca tcgcaaatga tcaatacctt gcagatccag tttcatcggc
     6301 ttattaagca tcaggttgaa tactaaaaag gcaaaaatca cctttctgga ataagcaatt
     6361 ccatttgaat ataagagcca gctcacagtt ctgttaatct tgcgccaaca ctatgactgc
     6421 tacgcagtga tagaaataat aagatcagga gaacggggat gaactttcca ttaattgcga
     6481 acatcgtggt gttcgttgta ctgctgtttg cgctggctca gacccgccat aaacagtgga
     6541 gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt ggtttttggc cttgccctgc
     6601 ataccattta tggttctgac agccaggtac ttaaagattc tgtacagtgg tttaacatcg
     6661 ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat gccgttagtc ttcgcctcta
     6721 ttctgagcgc ggttgcccgt ctgcataacg catctcagtt aggcaaaatc agttttctga
     6781 ccatcggtac gcttttgttt accacgctga ttgcggcgct ggtcggtgtg ctggtcacca
     6841 acctgtttgg tttgacggct gaaggtctgg ttcagggtgg tgcagaaact gcacgtctga
     6901 acgccattga aagtaactat gttggtaaag tctctgatct gagcgttccg cagctggtct
     6961 tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg agccaatccg acgtcaatta
     7021 tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc attgaaactg ctgaaggatg
     7081 atgcgccgaa aggtgaacgc gtcttaaccg ctatcgatac cctacaaagc tgggtgatga
     7141 aactggttcg ccttgtcatg cagttgaccc cttacggcgt tctggctcta atgaccaaag
     7201 tggttgcagg ttctaacttg caagacatca tcaaactggg aagtttcgtt gtcgcgtcct
     7261 acctcggtct gctgattatg tttgcagtgc atggcattct gctgggcatt aatggcgtga
     7321 gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac gtttgccttt accagccgtt
     7381 ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac gcgtcgtctg ggcgttcctg
     7441 aatccatcgc cagtttcgcc gactctttcg gtgcaaccat tggtcagaac ggctgcgccg
     7501 gtttgtatcc ggcaatgctg gcggtgatgg ttgcgcctac ggttggcatt aacccgctgg
     7561 acccgatgtg gattgcgacg ctggtcggta ttgttaccgt tagttccgca ggcgttgccg
     7621 gtgtcggtgg tggtgcaact ttcgccgcac tgattgtgct gcctgcgatg ggcctgccag
     7681 taaccctggt ggcgctgtta atctccgttg aaccgcttat cgacatgggc cgtac
//
LOCUS       DADLSB010000170         7629 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.170, whole genome shotgun sequence.
ACCESSION   DADLSB010000170 DADLSB010000000
VERSION     DADLSB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.170"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(267..4169)
                     /gene="hrpA"
                     /locus_tag="JN331_003507"
     CDS             complement(267..4169)
                     /gene="hrpA"
                     /locus_tag="JN331_003507"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY7261547.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4370..4966
                     /gene="azoR"
                     /locus_tag="JN331_003508"
                     /pseudo
     CDS             4370..4966
                     /gene="azoR"
                     /locus_tag="JN331_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
     gene            complement(5020..6330)
                     /locus_tag="JN331_003509"
     CDS             complement(5020..6330)
                     /locus_tag="JN331_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HAY7261548.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWSHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALPYVVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKL
                     PPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAVLDVTFEF
                     PRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALV
                     VAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6320..>7629)
                     /locus_tag="JN331_003510"
     CDS             complement(6320..>7629)
                     /locus_tag="JN331_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HAY7261549.1"
                     /translation="ARPALALWHRLRGLFFINSYVKGRYLTHDRQRVASFNNDPLITR
                     AIAVNILLDLYKTSERIVSDAAAITLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKEL
                     HLLPGFYHDTLGEENRAQAFEKMQSFICRLYANKSQKFDYQHEDRTGPSADRWRLLSG
                     GPVPLSPVDLAYRFMRKAMKLFGAHSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFG
                     RLIDKIYLNSVGWRGIRQRKTHLQILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDAL
                     ANEPAVSDILLRDYSELNVAQGQEMIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLA
                     IVSGLYELFPENEQVKNSLAGLANAIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKP
                     WVMRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT         1860 a         2086 c         1841 g         1842 t
ORIGIN      
        1 ctccttatcg ttcccttaag aatactgcgt gagataatag tgagaagcgg agcactctga
       61 aaggaggaag tcgtatttcc ttaaccgcca ctccgcaagc ctgacatatc ttttaaggaa
      121 atgctgtaga taaacattat tcatcttgca gctgagaatt gtatttttct gaattcctga
      181 atttttgtcc ttaccgtttt agaattaata aaaaacccgg cgctaaatgc ttaccgggtt
      241 ttagatttat taggcaaata gcagggttaa ccgctaatct gctccatcgc ctgcaaaata
      301 cgcttatctg aaatcggata aggcgtacca agttgttgag cgaagtaact aacgcgcagc
      361 tcttctatca tccaacggat ctctttcacg tcttcatcct cacgacgtgc aggcggcagt
      421 ttgttgatcc attgctgcca cgcctgctgg acgttttcga ctttcagcat ctgtgcacga
      481 tcgcgatgtg gatcaaccgc cagtttttcc agtcgttttt caatcgcctg caaatatcgc
      541 agcgtgtcgc ccagccgttt gaagccgtta ccagtgacaa aaccgcgata taccaacccg
      601 cccatctgcg ctttaatgtc agaaagcccc agtgccatgg tcatatccac ccgccctttc
      661 agacgtttgt tgatattgaa caccgcagtg aggatttgct cgacctgctt cgcaatatcc
      721 accaccgtgt cattcagttc ggcgcgcact ttttcatgca gcgcagcaaa gccttcttcc
      781 gtccagaccg ggccaccatt ggcgtcgatc agttgatcca caccgcagga gatacagtcg
      841 tcgatcagct ccagcacttt gccatacggg ttaaagtaca gccccagctt ggctttgttt
      901 ggtaacttct catgtaaata cttgatcggc gaggaaatat ttagcagcag tagacggcga
      961 agaccattcc acattgcctg tttttgctcc agcgggttat caaacagttt gatcgccacg
     1021 ctgtcgcgtt catccaccag cgccggccac gccttcactt tatagttgcc acgcttctgt
     1081 tcgtaacttt ccggcagctg accaaaactc cagatatgta agccgctctg ctcgataccg
     1141 tcatccgcca ccgcggacag cgtttcctgc actttgcctt tcagcgcatc tttcagatct
     1201 tgtagcgagc gcccttcttt tagcttcttg tttttgtcat ccaccacgcg gaaggtgatt
     1261 ttcaggtgat cgggcacctg atcccagtgc cagtcttcgc ggtcaacggt aacgccggtc
     1321 atccgccgta actcgcgctc aaggctgtcg agcaacggta actccagcgg tgtgacgcgg
     1381 cctaaaaacg cttcggcgta gtttggtgca ggcacaaaat tacggcgtac cggtttcggc
     1441 aacgatttaa tcagagcaat caccaattcg cggcgcagac cggggatctg ccattcaaac
     1501 ccgctttcct caacctggtt aagtaacggc agcggaatat gtacggtcac accgtccgcg
     1561 tccgcgcctg gctcaaactg atagctcaaa cgcagcttga gattgccctg atgccagaag
     1621 ttcgggtaat ccagcttgct gattttttcc gccccttctt tgatcaacat gctcttttca
     1681 aagttgagca aatcaggcgt ttcgcggctg acttttttcc accagctatc gaagtgacga
     1741 gcggagatta catcgtggct gatgcgctgg tcgtagaact caaacagcgt ttcgtcatca
     1801 accagaatat cgcggcgacg tgatttgtgt tccagctctt ccacttcggc ccgcagtttc
     1861 aggttttcac ggaagaatgc gtgacgcgtc tgccagtcac cttccaccag agcgtggcga
     1921 ataaagagtt cacgacataa cgccgaatcg atctggctgt agttgacctt gcgcgcagca
     1981 acaatcggca aaccataaac agtgactttt tccgttgcca tcaccgcgcc ctgcgcccgt
     2041 tcccagtgcg gttcgctgta ggtgcgttta atcaaatgct gagcaacggg ttctacccat
     2101 tccgggtcga tacgcgcagc aatgcgcccc cacaggcggc tggtttctac cagttccgcc
     2161 accattaccc atttcggcgg ctttttgaat aaaccagaac cggggaagat ggagaaacgc
     2221 gcgttacgtg caccggtata ttcctgttta tcggcatctt tcatgccgat atgggaaagc
     2281 aaaccggtca gtaaggcgat gtgaatttca cgatactccg ccggttcgct gttgaccgga
     2341 atgccaagtt ctttcaccac ctggcgcaac tgggtgtaga tatcctgcca ttcgcgcacg
     2401 cgcagatagt tgaggtaatc ggtacgacac aggcgacgga aggcgttgga agaaagcgcc
     2461 ttttgctgct cgccaagata attccacaga tttacaaacg cgaggaagtc ggattctttg
     2521 tcgtggaagc gacgatgttt ttcgtccgat gcctgctgtt tgtccatcgg acgttcgcgc
     2581 ggatcctgaa tggagagcgc ggacgtgata atcatcgcct cacgcacgca gccatgtttt
     2641 tgcgcttcca gcaccatacg cgccagacgt gggtcgacag gcaactgcga gagctggcga
     2701 ccgagcggcg tcagtttata ggcgctggcc tgttcatccg tggtgatcgc gcccagttct
     2761 tcgagcagac gcacgccatc ctggatattg cgtttatccg gtgcttcgac aaacgggaac
     2821 gcagcgatat cgcccagccc cagcgcggtc atctgcaaaa taaccgaggc caggttggta
     2881 cgcagaatct ccggatcggt aaactccggg cgcgagagga aatcgtcttc ggaatagaga
     2941 cgaatacaga tcccttcgga cacacgacca cagcggcctt tacgctgatt ggcagacgcc
     3001 tgcgaaatgg gttcaatcgg caaacgctgc actttggtgc gatagctgta gcggctgata
     3061 cgcgctgtac cggggtcgat gacgtattta atccccggta cggtcagcga ggtttccgcg
     3121 acgttggtcg ccagcacaat gcgccgtccg ctgtgcgact ggaacacgcg gttctgttcg
     3181 ctgttcgaaa gccgcgcata aagcggcaag atttcggtat ggcgtaagtt cagcttgttc
     3241 agcgcatcgg cggtatcgcg aatttcccgc tcgccgctca taaagatcag aatgtcgccg
     3301 gggctttcct ggcttagttc gtctacggcg tcaaaaatcg cctgcaactg atcgcgctcg
     3361 gtgtcatcgg cttcttcaac aatcgggcga tagcgcactt ccaccggata ggtccgaccg
     3421 gagacttcaa taatcggcgc attattaaag tggcgcgaaa agcgttccgg gtcgatagtc
     3481 gcggaagtga taatgatttt taggtcagga cgccgcggca gcaactcttt caaatagccg
     3541 agcagaaaat cgatattcag gctgcgttcg tgcgcttcgt caataatgat ggtgtcgtac
     3601 tgcatcagca ggcggtcttg ctggatctcc gccagcagga tcccgtcggt catcagcttg
     3661 accatcgtgt tatcacttac gtgatcgctg aaacgcactt tgtaaccgat gcaaccgccc
     3721 ggctccgttt tcagctcttc cgcaatacgg ttggccaccg ttcgtgccgc caggcgacgc
     3781 ggctgggtat gaccgatcag ccctttaata ccgcgcccca gctccataca gattttcggt
     3841 agctgagtcg ttttaccgga gccggtttcc ccggcgacga tcaccacctg gtgatcacga
     3901 atcgcttcga gtatgtcctg ttttttctga ctaaccggta aattgtcagg ataagtgatt
     3961 tccggtcgtg ccgcttcacg cagcaggact ttccctgccg cctggtcaat ctctttcgcc
     4021 atctcctgga aaatggcctg ttgtgcatca ggatttttaa ccttcttaac acaatgcaga
     4081 cggcgagaaa aacgcagtct gtcgcgcagc atcagcgaat ccagccgctg ttgcaaggca
     4141 gtaaaggtca atttttgttg ttctgtcata acgttagtgg gcagtagtct gcctgattaa
     4201 ttttctttta aatgatagat atagagtacc acatcgcggc tttctatgcg ttgttcaata
     4261 aattcgaaca tagacttcga tttattgcgc tatctctgtg gcaggattgt gaataaagtg
     4321 tcagcaagca acggggcatc gtccattcaa acatctataa ggaaacacca tgagcaaggt
     4381 attagttctt aaatccagca tcctggcagg gtactctcag tctaatcagt tgtccgatta
     4441 ttttgttgaa caatggcgcg aaaagcactc cgctgatgaa atcaccgttc gcgacctagc
     4501 tgcaaatccg attccggtac tggatggcga actggttggc gctctgcgtc cgagcgatgc
     4561 gccgctgact ccgcgtcagc aggaagctct ggcgctttcc gatgagctga ttgccgagct
     4621 gaaagcccac gacgttatcg ttattgcggc accgatgtat aacttcaaca tctcaactca
     4681 gttgaaaaac tattttgacc tggttgcacg cgcaggcgtt actttccgct ataccgagaa
     4741 cggtccggaa ggtctggtaa cgggtaaaaa agccatcgtt attaccagcc gcggtgggat
     4801 ccacaaagat ggaccaacgg acctggtgac gccgtatctg tccacggtcc tcggctttat
     4861 cggcattacc gatgtgaaat ttgtcttcgc cgaagggatc gcatacggtc cggaaatggc
     4921 agcgaaagca cagtctgacg caaaagcagc catcgacagc attgttccgg acacggtctt
     4981 gatgtggact gtagatattc agtccacatc tcaatccact tacctgtttt cccataattt
     5041 caacatcgct ttgtgctctt ccttaagcac aatatgcgag cgtctggctc gaataaagct
     5101 aatcgcttca tcaactgttt tacagtgtcc gtaacataac agccatgccg ccactaccag
     5161 cgcactgcgc gataatccca atgcgcaatg gaccagaacg ctgccttgct cttcgcgtaa
     5221 tgtttccagc atcgccacgg cctgtcggag ctccccctct tccggaacca ccagatccaa
     5281 catcggtaca cagaaataga gtcgatcttt tgtcgctcgt ccacgaggga attcaaaggt
     5341 gacgtccaga accgcattct gtgccggaat atgtcgtgga aacgccctta aataaacacc
     5401 agcagtcatt ttgctcaccg gctccaggcg acgacaaaac caacgcatag atagccacat
     5461 cccgatgcgc cagggcaatg tcagccagta aacggcgggc gggagtttcc cctgactatc
     5521 tttccctgtt gttatcgcgc caagcccacc gtacccacgg ccaataatga gtagcgataa
     5581 tactggccaa cataaccaga ctgaccacca taactgaacc atcatcatta gctccatcaa
     5641 cacaatgcac gcgcctacga catatggcag tgctattttg attcgacgtt gatcaagttt
     5701 ctgataattc caacgacggt cgacgggcac catccagtca atcaacatac ctaccgccag
     5761 ccctgtgatg acatcaataa aatgatgctg ccatgtcgtc agcgtcgaaa tggcgatgag
     5821 taaaaaccat ccgccacaga ctttacgcca cctcacagcc agatgctgac gaaagtgact
     5881 ccaaagtagc cagcagagaa taatatgcag cgaaggagac tggttataag gcagatcaaa
     5941 cagttcaagt tgcgaaaata gccatcctgt caccccactc acttcaggac ggataaaact
     6001 aaacttcagc gggtagagaa aaaaaccgca gcaggccatt accgttgcca gaataagccg
     6061 gtggacaagt cggcgttgtt cgaatgtcga gctacaaacg aacagcgaaa atccatataa
     6121 aagatccaga ctccagtaag gaacaatagt ccagggaaga aaagggatcg ccgtttccca
     6181 accgaatacc tgactgggga tatcatggct gttaaggtcc tgaaccgcgg tgaactgatt
     6241 aagagatcca taggtgaaga agaaaaacgg ggccagcaac aataaccagc cagcgccttg
     6301 tagcaatacg ttccgacgtt cagttatcac gacgcaccgc catcgaaacc gtaaaaatgc
     6361 cccacacatc aatccgttga gtgcatttat caaatccggc attacgcacg agcgaatcca
     6421 tctccccttg cgaacgcacg cgcattaccc acggtttacc atctttatga ctggttaaca
     6481 ccccggcaat cagctccagt tgtgggtgcc acggctgccc ggtgtagatg agaatgccac
     6541 ccggttcgat ggcattggct agacctgcga gtgagttttt tacctgctca ttttcgggaa
     6601 aaagctcata taggccagag acaatcgcca gcgtaggccg cggagttaac gcgctgagtt
     6661 cctccgggtt aaacgcatcg ccctgttcaa aacgcacccg cccagacatt ccccgttggg
     6721 caatcatctc ttgcccctgt gcaacattta actcactgta atcacgtaac aaaatatcgc
     6781 ttacggcagg ctcgtttgcc agcgcatcca gtacatagcg tccatgccct gcggcaatgt
     6841 caaccacgcg gacggctaaa cctttggcgt ggagatcggc aacggcttgt ttaatcaata
     6901 tttgcaaatg ggttttgcgc tggcgaatac cgcgccatcc aacactgttc aggtagattt
     6961 tgtcgattaa acgcccgaat gcgttactgc cttgcggttg attttgatag acataatcca
     7021 gggaactgcc tgaatcaaag ccggtactca ttccgagatg caggcccgca gagtgcgcac
     7081 cgaacaattt catcgccttt cgcataaagc gatacgctaa atcaaccggc gataatggca
     7141 cgggtccacc agaaaggagc cgccatcgat ccgctgatgg tcctgtgcgg tcttcatgct
     7201 gataatcaaa tttttgcgat ttgttagcat ataagcggca gataaagctt tgcatttttt
     7261 caaatgcctg cgccctgttc tcttcaccca atgtgtcgtg ataaaagcct ggcagcagat
     7321 gcagctcttt cagagggcta cgtaatctct gataaaaatc aatctgcggt tggcgatgca
     7381 ccacatagtc atcacctgat atcagaagtt gcgtggggag cgtaatcgcc gccgcatcgc
     7441 taacaatacg ttcagacgtt ttatagagat cgagcaagat attgacggca atcgcccgtg
     7501 tgatcagcgg atcattattg aaactcgcca cccgttgccg atcgtgggtc aaatagcgcc
     7561 ctttcacata ggaattaata aaaaatagac cacgcagacg atgccataac gccagcgcag
     7621 gacgtgcca
//
LOCUS       DADLSB010000171         7527 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.171, whole genome shotgun sequence.
ACCESSION   DADLSB010000171 DADLSB010000000
VERSION     DADLSB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.171"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            556..1326
                     /locus_tag="JN331_003511"
     CDS             556..1326
                     /locus_tag="JN331_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY7261550.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1317..1790
                     /locus_tag="JN331_003512"
     CDS             1317..1790
                     /locus_tag="JN331_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY7261551.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1897..2436
                     /gene="dnaT"
                     /locus_tag="JN331_003513"
     CDS             1897..2436
                     /gene="dnaT"
                     /locus_tag="JN331_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY7261552.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2439..3176
                     /gene="dnaC"
                     /locus_tag="JN331_003514"
     CDS             2439..3176
                     /gene="dnaC"
                     /locus_tag="JN331_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY7261553.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3225..3719
                     /locus_tag="JN331_003515"
     CDS             3225..3719
                     /locus_tag="JN331_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY7261554.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3973..6264
                     /gene="opgB"
                     /locus_tag="JN331_003516"
     CDS             3973..6264
                     /gene="opgB"
                     /locus_tag="JN331_003516"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY7261555.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6402..7424)
                     /locus_tag="JN331_003517"
     CDS             complement(6402..7424)
                     /locus_tag="JN331_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY7261556.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
BASE COUNT         1913 a         1951 c         1833 g         1830 t
ORIGIN      
        1 tttccagcaa attaatagat taagcgaata aattatcctt agcattcact ttcatatcag
       61 catactcctg ctgcacaaca actccactgc atgtgtcaga aggttagcat caaatgataa
      121 cctggtgaaa tagcctggag acatttaaaa tatctacatt aagttgttag aaatacttaa
      181 tgaaaaacag aaatctcatt catttaattt ataattaaat gatatctctt cccgcagaat
      241 gcgggaatca cacaatcaat atttattcaa taaaatctga tgattttaac ttgttaacac
      301 taaaaaacgc gcccagctat ctattatcct gacccgtaaa ggctaaacaa taaattcatt
      361 atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc tttttatcac
      421 cgagcttgtc ccaataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa
      481 caaccgtgct tcatgataca aatggattct cattatctga acaatacgca gcacgtctat
      541 gacaaagggc gagttatgca aactgagcaa cagcgagccg taacgcggct ttgtatccag
      601 tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca
      661 cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc gaacgccata
      721 gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc
      781 ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc ggagcatcat
      841 ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcaaccatt acgttaccca
      901 agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa actcaataac
      961 ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat gtatatccgc
     1021 cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac cgctttcgcc
     1081 gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac ccccaccatt
     1141 gcgatggccg ccagcgttct gctgctagtg ccgggctttc cgttgattaa tgccgtcgcc
     1201 gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg
     1261 acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct acgcggatgg
     1321 gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc attcctgcgg
     1381 tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt gcgctgcttg
     1441 gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat attgagtggt
     1501 caacctttat ggcttctatg ctggtcggta ccattggtat tcaatggtcg cgctggtatc
     1561 tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg
     1621 cttataccgc aatgatttcg gcggtaaaaa tcagccagtt aggttacagc gaaccgttga
     1681 tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg
     1741 gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg
     1801 cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
     1861 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
     1921 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa
     1981 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
     2041 cgcctggctg aactgctggc gctggaagaa aagctagcgc gtccgggaag cgatgtcgct
     2101 ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
     2161 ttcgccatgt atccggactg gcaacccgat gccgatttta tccgcctggc ggcgctatgg
     2221 ggcgtggcgc taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg
     2281 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
     2341 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagtgaa
     2401 cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
     2461 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag
     2521 agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc gaacgtgaaa
     2581 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga
     2641 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
     2701 cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc
     2761 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
     2821 aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga
     2881 atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
     2941 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
     3001 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
     3061 agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttat
     3121 gggtgatctt caactgggat agctaccgta gccgggtaac gggtaaagag tattaatccc
     3181 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactatta
     3241 aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
     3301 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
     3361 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccagg
     3421 ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa
     3481 agctggcttc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
     3541 tgaacagtga agagcaaaaa gaagacacca actatctgga cggtattcag ggtttgctga
     3601 aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa
     3661 aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa
     3721 ctgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct gcccttttcc
     3781 ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa aaaagtctgt
     3841 ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc
     3901 gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt
     3961 gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt
     4021 tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacgttaac ggtgctgggg
     4081 ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt
     4141 aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac
     4201 atcctgccgg gtatcggcat tgtgctgggg ctgacggcgg tgttcggtgc gctgggctgg
     4261 atcctgcgcc gtcgtcgtca tcatccgcac cattttggtt acagcctgct ggcgctctta
     4321 ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga actggtgaaa
     4381 tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact
     4441 atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat
     4501 tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc
     4561 ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg
     4621 gcttctcagt gcggcatacc gctgttcgcc ccctttgaag gcaacgcctc cgcctctgtc
     4681 tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag
     4741 aactatttcg tgcagggcgc aaatctgcgt tttgccggta aagatgtgtt cctgaaatcg
     4801 cacggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtagc cgacccgcac
     4861 tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt
     4921 gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat
     4981 cacccggatg gttttatctc gcgcacctgt aaccgcaaaa aatatgattt tgacggtaag
     5041 ccgaatcagt cattcagcgc ggtaagttgc agccaggaga atatcgcgac gtttatcaac
     5101 aaaatcaaag cgtcaccgtg gtttaaagat actgtcatcg tcgtctcttc tgaccattta
     5161 gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa cctgtttttt
     5221 gtcattcgtg gcgacaagcc gcagcaaaag acgctggcag tgaagcgtaa cacgatggat
     5281 aacggcgcga cggtgctgga cattctcggt ggcgataact atctcggact tggtcgtagc
     5341 agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg
     5401 tggaagccag atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc
     5461 gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg
     5521 cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt
     5581 ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgacag ttgctacaag
     5641 atggcacaac tctgggctcc ggaactggca ctttccaccg actggtgtgt ctcgcagggg
     5701 cagcttggcg gccagcaaat tgttcagcat gttgacaaaa caacatggaa gagcaagacg
     5761 gcatttaaag atacggtcat cgacatggcg cgttacaaag gcaatgtcga tacgctgaag
     5821 attgttgata acgatattcg ctacaaagcc gacagtttca tctttaacgt cgccggtgcg
     5881 ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg ccgctggtcc
     5941 aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc gaagaaattt
     6001 gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc
     6061 gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac cacactgcat
     6121 ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc
     6181 aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat ggtggaaatt
     6241 aaagtggtag aacgtgaagg gtaattcctg actgatgtgt aggtcgcata gggtgcgacc
     6301 tacacgcctt ctccggctta caacacatta ttaagaaaca aaaaaaacag cgcccaacac
     6361 ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt aattacaccc
     6421 ttaatcaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac
     6481 ggatagcgat gggttaacat catgtcggca gtgattttcc cttccgccat cagtcgaccg
     6541 actttagcga aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt ttctttttta
     6601 tggaattccg gatcggagaa ctgcaactca cctttaaaca gaccgacaaa taccaccgtg
     6661 ccgccgtgac gaatcagatt cacggtgtta ttcatcgcat gttgattacc tgtcgcgtcg
     6721 atcactttct gcgccagcga accaccaaac tgcgcccgca gctgcgcgtc aaaatcttcg
     6781 gctgacgggt ccagcacggg caactccaga cgcgttgcca catgttcacg gcgcgccgga
     6841 ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc cgcgcccaga
     6901 ccgattggcc ccgccccgac caccagcacc tgctcgccgg gagcaatggc tgcgcgacgc
     6961 accgcatgag cgctaatagc gaaaggttca atcaatgcgg ccgcctgcgg gtcaataccg
     7021 tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga
     7081 tgcacgccaa tgactgaaat tttttcacag cagttggtac gcccgctttt acacgccggg
     7141 cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt aagatcagca
     7201 atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac ccgtggataa
     7261 ctaaaaaatg gttgatttcc accccaggca tgaatatcgg taccgcaaat cccgacagac
     7321 ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg tttccagact
     7381 aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg gctctccatt
     7441 ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa aatctgtttt
     7501 gtgaaaacac acgcataaaa atgtttt
//
LOCUS       DADLSB010000172         7506 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.172, whole genome shotgun sequence.
ACCESSION   DADLSB010000172 DADLSB010000000
VERSION     DADLSB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.172"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..1183
                     /gene="pgaB"
                     /locus_tag="JN331_003518"
                     /pseudo
     CDS             1..1183
                     /gene="pgaB"
                     /locus_tag="JN331_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
     gene            1176..2516
                     /gene="pgaC"
                     /locus_tag="JN331_003519"
     CDS             1176..2516
                     /gene="pgaC"
                     /locus_tag="JN331_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HAY7261557.1"
                     /translation="MINRIVSYRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPF
                     FMSIMWIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAA
                     LAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQKQGKAIALKTGAAAAK
                     SEYLVCIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSI
                     IGLIKRTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIF
                     YEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLT
                     TIWAFTCLAGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRY
                     EHNLTSSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            2518..2931
                     /gene="pgaD"
                     /locus_tag="JN331_003520"
     CDS             2518..2931
                     /gene="pgaD"
                     /locus_tag="JN331_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HAY7261558.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(2981..3904)
                     /gene="phoH"
                     /locus_tag="JN331_003521"
     CDS             complement(2981..3904)
                     /gene="phoH"
                     /locus_tag="JN331_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAY7261559.1"
                     /translation="MRSRFSAFYPKANYGLQGSQPSDVRVHNRAANGACDEYKQLKVL
                     SMGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPI
                     LARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQ
                     ADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVETAPFAYMRGR
                     TFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVCSGLSDALER
                     FEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(4377..5642)
                     /gene="efeB"
                     /locus_tag="JN331_003522"
     CDS             complement(4377..5642)
                     /gene="efeB"
                     /locus_tag="JN331_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAY7261560.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(5648..6775)
                     /locus_tag="JN331_003523"
     CDS             complement(5648..6775)
                     /locus_tag="JN331_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAY7261561.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(6833..>7506)
                     /locus_tag="JN331_003524"
     CDS             complement(6833..>7506)
                     /locus_tag="JN331_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HAY7261562.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1882 a         1921 c         1773 g         1930 t
ORIGIN      
        1 aaatgcgtca caattggatt ccattccgcg agtattaatc gccaataatc cctcattaaa
       61 agagtttgcc cagcaaatta ttaccgtaca ggaaaaatca ccacaacgga taatgcatat
      121 cgatcttgat tacgtttatg acgaaaaccg ccagcaaatg gatcgcaata ttgatgtgct
      181 aattcagcgg gtgaaagata tgcaaatatc aaccgtgtat ttgcaggcat ttgctgatcc
      241 cgatggtgat gggctggtca aagaggtctg gtttccaaat cgtttgctac caatgaaagc
      301 agatattttt agtcgggttg cctggcaatt acgtacccgc tcaggtgtaa acatctatgc
      361 gtggatgccg gtattaagct gggatttaga tcccacatta acgcgagtaa aatacttacc
      421 aacaggggag aaaaaagcac aaattcatcc tgaacaatat caccgtctct ctcctttcga
      481 tgacagagtc agagcacaag ttggcatgtt atatgaagat cttgccggac atgctgcttt
      541 tgatggcata ttgttccacg atgatgcttt gctttcagat tatgaagatg ccagtgcacc
      601 ggctatcacg gcttatcagc aagcaggctt tagcgggagt ctgagcgaaa ttcgataaaa
      661 cccggagcaa tttaaacagt gggcccgctt taaaagtcgt gcgttaactg acttcacttt
      721 agaacttagt gcgcgcgtaa aagccattcg cggtccacat attaaaactg cacgaaatat
      781 ttttgcactt ccggtaatac aacctgaaag tgaagcctgg tttgcacaga attatgctga
      841 tttcctaaaa agctatgact ggaccgctat tatggctatg ccttatatgg aaggtgtcgc
      901 agaaaaatcg gctgaccaat ggttaataca attgaccaat caaattaaaa acatccctca
      961 ggctaaagac aaatctattt tagaattaca ggcacaaaac tggcagaaaa atggtcagca
     1021 tcaggctatt tcttcgcaac aactcgctca ctggatgagc ctattacaac tgaatggagt
     1081 gaaaaactat ggttattatc ccgacaattt tctgcataac caacctgaaa tagaccttat
     1141 tcgtcctgag ttttcaacag cctggtatcc gaaaaatgat taatcgcatc gtatcgtatc
     1201 gtatcgtatc gttttttata ttatgtctgg tgttatgcat acccctatgc gtagcgtact
     1261 ttcactctgg tgaactgatg atgaggttcg ttttcttctg gccgtttttt atgtccatta
     1321 tgtggattgt tggcggcgtc tatttctggg tctatcgtga acgccactgg ccgtggggag
     1381 aaaacgcacc agctccccag ttgaaagata atccgtctat ctccattatc attccctgtt
     1441 ttaatgagga gaaaaacgtt gaggaaacca tacacgccgc tttagcacag cgttatgaga
     1501 acattgaagt tattgccgta aatgacggtt caacagataa aacccgtgcc atcctggatc
     1561 gcatggctgc acaaattccc catttgcggg tcattcatct ggcgcaaaaa caggggaaag
     1621 ccattgcgct taaaaccgga gctgccgcgg cgaaaagtga atatctggtg tgcattgatg
     1681 gcgatgcgtt attagaccgc gatgcggcgg catatattgt ggaaccgatg ttgtacaacc
     1741 cgcgtgtggg tgccgtaacc ggtaatcctc gtattcgaac acgttctacc ctggtgggta
     1801 aaattcaggt tggcgagtat tcctcaatta ttggtttgat caagcgaacc cagcgtatct
     1861 atggaaacgt atttaccgtt tccggtgtta ttgccgcatt tcgtcgcagc gccctggcag
     1921 aagtgggtta ctggagtgac gatatgatca ccgaagatat tgatattagc tggaagctgc
     1981 agttgaatca gtggacgatt ttttacgagc cacgggcgct gtgctggata ttaatgcctg
     2041 aaacgttaaa agggctgtgg aaacagcgcc tgcgctgggc tcagggcggt gcagaagtat
     2101 tcctcaaaaa tatgacaagg ttgtggcgca aagaaaactt tcgaatgtgg ccgctgtttt
     2161 ttgaatactg cctgacgaca atatgggcct tcacctgcct ggccggtttc attatttacg
     2221 cagtccaact tgccggtgta ccgttaaata ttgaattgac acatatcgct gcgacacata
     2281 ctgccggaat attattgtgt acgttatgtt tactgcaatt tattgtcagc ctgatgatcg
     2341 agaatcgcta tgagcataat ctgacttcat cgcttttctg gattatttgg ttcccggtta
     2401 ttttctggat gctgagcctg gcaacgacat tggtatcatt tacacgagtc atgttgatgc
     2461 ctaaaaagca acgcgcccgt tgggtaagtc ctgatcgcgg gattctgaga ggttaatatg
     2521 aacaatttaa ttattacgac ccgacaatca ccagtacgtt tactggttga ttatgttgcc
     2581 acaaccatct tgtggacatt atttgctttg ttcatattct tattcgccat ggatctgctg
     2641 acgggttatt actggcaaag cgaggccaga agccgacttc agttctattt tttgctggca
     2701 gtggcgaatg ccgtcgtgtt aattgtctgg gcgctgtaca ataagctgcg ttttcaaaaa
     2761 cagcagcatc atgcagccta ccaatatacg ccgcaagaat atgcagagag cttagcaata
     2821 cctgatgagc tctatcagca actacaaaaa agccacagga tgagcgtaca cttcaccagc
     2881 caggggcaaa taaaaatggt tgtttcagaa aaagcgctag tccgggcata aacacccaaa
     2941 acaaagcccg gttcgcccgg gctctgcacc gataatacac ttaactgtat gcatgcagcg
     3001 tacgttggca aagtgccgaa cgtacgcaat cctcttttcc gaaccggacg atcccaacca
     3061 tttcatcttc ttcgaaacgt tccagcgcgt cacttaatcc ggagcacacg ccgcgaggca
     3121 aatcgcattg cgtgatatca ccgttgacga taaccgtcac gttctccccg aggcgggtta
     3181 aaaacatttt catttgcgcg gcagtcacat tctgcgcctc gtcaagaatg acgactgcat
     3241 tttcaaaggt acgtccacgc atataggcga acggcgcggt ttccaccttc cctatttccg
     3301 gtcgcaggca gtactgcata aaggaagccc ctaagcgccg gaccagcacg tcgtagaccg
     3361 ggcgaaaata gggagcaaac ttttctgcga tatcaccagg taagaagcca agatcttcat
     3421 cggcttgcag aactggacgg gtgacgataa tcctgtcgac atccttatgt atcagggcct
     3481 ctgccgcttt tgctgcgctg atccaggttt ttccgcaccc ggcttcgccc gtggcgaata
     3541 tcagctgctt actctcaata gcctgcagat agtgcaattg cgcttcattt cgcgcaagga
     3601 tgggcgaggt atcgcgactg tcgcgggcca taccaatggc ttctacgccg ctcatctgca
     3661 caagcgaggt gaccgattct tcttcacgct gcttatggct gcgcgaatcc cgtctcagca
     3721 cacgttttgc ctcgcgacga gctttgatca ctgctttttg tcttcccatg gagagcacct
     3781 tgagttgttt gtattcatca cacgcgccgt tggcagcgcg attatgcaca cgaacatcag
     3841 agggttggct tccttgtaag ccatagtttg cttttggata aaatgccgaa aaacggctac
     3901 gcacaccgtt tgcggcgtcg gtaacacgag taaagaaagg aagtgagaaa aatacagagt
     3961 gacgaagttg ctaccggagg agaaatctcc gcgagtggtg gaacgttgct taacaaaaca
     4021 ttgtccagaa caggacgtta ccatccgcga tctccatagt gactgactat cactgccggg
     4081 aacttccgct gctacttaat aagtacaaca gaactcgcat tcattgcaac aatatattca
     4141 cttatattta acgataaata cccatttcgg tgacattaat ttctactgga aagatgacag
     4201 agtgatgaca gtgatggaaa aagctgtgtg ctttccgcag gattcgcagg gctggtaaaa
     4261 tgtgcctcgt gtttgccgga ttcggcgcga acgccttacc cgacctacaa gtgattaata
     4321 atttgtgcac aaaactcatg cccgataaac ctgcgcatcg ggcatatttg cgtcgttcaa
     4381 acctgcaata gcgcccttcc cagataatcg ttcgcgtcct tcactcccgg cagcgaaaaa
     4441 aaataaccgc cgccgatagg tttaacgtat tcctccagcg cttcgccatt gagccttttt
     4501 tgtactgtca ggaagccttt ttccagatcg tgttggtagc agacaaacag caaccccata
     4561 tccagttgtc cggagttggt gacccccaac gaataactgt agccgcgacg cagcatcagg
     4621 ctggactcac tctccgccgt gcggggattc gccagccgga tatggctgtc cagcgcgatc
     4681 accttccctt ccgggtcgct ggcgtaatca ggcacatcat gctcatgctg cattcccagc
     4741 ggcgcaccgg tttgcttatc acggccaaaa atcgtctgct gttctttcag cggcgttctg
     4801 tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct gatagctgcc accgattgtc
     4861 cacgcaggct cctgctgatc tgccgttacc cacaccactt tttgcatcaa cttatcattc
     4921 tggctatcgg gattggcagt gccgtctttg aaacccagca aattaatcgg cgtctctttg
     4981 cctttactac gcgccgcgtg atcggaaata aacccttccc gcttccagcg cacactgagc
     5041 aaatccggcg tgtgtttgat gatatcgcgc agcgcatgga tcaccgtgtc ctgggtgttg
     5101 gcgcaaatct gtagcaacac atcaccatga cataacgccg catccagcga gtcgttgggg
     5161 aaacgcgtca tcttctgcag cttttttggc atctgtggcg caaggccaaa gcgctcatca
     5221 aacaatgagt gacccaccga taacgtgatg gtgagattat cgggcgcaat gtagccgcca
     5281 agaatgccgg aatcgagtgg tggcaggcgc ggattcggcg tttctggtgc tgctccgccc
     5341 tgactcagaa aagcaaaacg ctgagtcaac aagcgaaaca accgctcaag atcggcttta
     5401 tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg cctgttgtgg cgtcaggatc
     5461 cctgcctgat gctcaccata aaacggctgt ttctcattgc gtgcatccgg tgaaagcgtg
     5521 cccggtgcac tttgcgtttt ttgtgcatga gcgaccggac aacttcccgc cagcgcacca
     5581 atccctttca gtaaacgtcg gcgtgacggt tcattcacgc cgttttcatc tttatactgc
     5641 ataacgctta atccagtccc agcacaccgc gaagttgcgc cagatcttcc gccagcgcag
     5701 taatcggccc tttcagcgca ttccggtcgg catcggtcaa tttgtcgtag gtttcaaaac
     5761 cgtctttagt acggtatttc gccagaatgg tatcgacctt tttaaagttg gcatcgactt
     5821 ttgccaacag ttccgggttg gctttttgca gttgtggacg cagcagatcg acaattttct
     5881 gcgagccttc aacgttagcc tggaaatccc acagatcggt gtggctgtag cgatcttctt
     5941 caccgctgat tttgctggct gccacttcct caatcagtcc ggctgcgccg ccgaccactt
     6001 ttgaaggtgg gaaagccagt tcactgatgc gtttttgcaa atcgaccaca tcggtataaa
     6061 gctgctcagc gtactgatcc atccctttgg tggtgttgtc gccaaacaat gctttttcca
     6121 gacggtggaa accagtgaat tttgggtcgg cggctttttg ctcgtaatca tcttcacggg
     6181 cgtcaatgct gccatccaga tcggagaaca gttcagcaat cggttcaata cgctcatagt
     6241 gctggcgcgt cggcgcatac agtgctttcg ctttttcgat atcgcctgct ttaatcgcgt
     6301 cggtaaaggc tttggtgtcg gtcaccagct gcgtggtttc cgccatgaca tacgctttat
     6361 atgcagtaat tgcaccacca agacttaaca gcgcatcact ttgcgccgca tccgccgttg
     6421 cctcaccttt gacgatcaac ttccctttcg ggttagtcag cagaccgcag gtcatatcgt
     6481 attcgccagg ctgtaaattc gccgtcattt tctggctaaa accaggggcg atattttccc
     6541 gctcttccac caccatcacg cctttgagga tctcccactc cagcgccttc tggctatggt
     6601 tctgaataat gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt tcgcactgct
     6661 tatccgtcac ggtcactttg acctgcggca catcagcggc gttagccata aaagcagaag
     6721 aaaacagcgc agccacgctc aactgcaatg cgttacggcg gaagttaatg gtcatgacga
     6781 gactatccct ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt tgttacgcgg
     6841 agcgagacgc tgtcgcccct gcgcgtggtg gcagagcaaa tgccaccagc gccgggatga
     6901 gataaataaa ccagacggcg acttcgctga cgctcggtgc ttcctgatag ccaaaaatcc
     6961 cttccatcag cgtgccaaac agcgagtgag ttgagaggac cgcgctcata tcgaaggcga
     7021 tttcctgaaa gtggttccac aatccggctt catgaaatgc gcgaatggca ccagccgcca
     7081 gccctgcggc gacgaagaga ataaacaggc tggtccattt aaaaaatgca ccaagattga
     7141 ggcgaatacc gccccagtag agcaggaagc cgagcaccac ggcagtagca agaccgagca
     7201 ttgcacccag cggcggccag atcccgacat cttgttgaaa tgccgccagc aggaaaaaga
     7261 ccgactccag cccttccctt gcaacggcaa aaaagaccat catcaccagc gcccagccat
     7321 gatgatttcc acgctgcaat gcgctatcga ctgcctgttc cagttgcact ttgacgttgc
     7381 gcgacacttt gcgcatccag aaaaccatcc aggtaaggat caccacggcg atcaccgcca
     7441 cgataccttc aaacagttcc tgctcttttt gcggaaattc gccggtggtt tcgttaatga
     7501 agatcc
//
LOCUS       DADLSB010000173         7494 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.173, whole genome shotgun sequence.
ACCESSION   DADLSB010000173 DADLSB010000000
VERSION     DADLSB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.173"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..544
                     /locus_tag="JN331_003525"
     CDS             <1..544
                     /locus_tag="JN331_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HAY7261563.1"
                     /translation="RYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVLDQPTM
                     KALDKQNVAQALSVVPGVVLQKSGSRNEKQVKVRGFDSRQVPVYFDGVPIYVPYDGNL
                     DLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVRTMPTI
                     CMLHLPPAAILGICKSAVAS"
     gene            481..1941
                     /locus_tag="JN331_003526"
     CDS             481..1941
                     /locus_tag="JN331_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7261564.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKE
                     SFYYQGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYFDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKEGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(1991..2245)
                     /locus_tag="JN331_003527"
     CDS             complement(1991..2245)
                     /locus_tag="JN331_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY7261565.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            2446..3180
                     /locus_tag="JN331_003528"
     CDS             2446..3180
                     /locus_tag="JN331_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY7261566.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYNLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(3182..3793)
                     /gene="emtA"
                     /locus_tag="JN331_003529"
     CDS             complement(3182..3793)
                     /gene="emtA"
                     /locus_tag="JN331_003529"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY7261567.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            3893..4807
                     /gene="ldcA"
                     /locus_tag="JN331_003530"
     CDS             3893..4807
                     /gene="ldcA"
                     /locus_tag="JN331_003530"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY7261568.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            4902..6638
                     /locus_tag="JN331_003531"
     CDS             4902..6638
                     /locus_tag="JN331_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY7261569.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            7249..>7494
                     /locus_tag="JN331_003532"
     CDS             7249..>7494
                     /locus_tag="JN331_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261570.1"
                     /translation="MASFKDICLFAPAIPPSSVNPGVRRPPGRLFLSVELKNQILQNM
                     KYLSPQKFSWGDAPWQIIDLSIAGKVNIQVDNNTIITL"
BASE COUNT         1820 a         1910 c         1905 g         1859 t
ORIGIN      
        1 acgttatctc tgcacagcat tatcacttgc ctttacccag caggccgtag cggctcagga
       61 gagcgacacg ctgaccgtat ggtccagtcc ggtatcatcg acgacgacca ccgttctcga
      121 tcaacccacc atgaaggcct tggataaaca gaatgtcgct caggcattaa gtgtcgtccc
      181 cggcgtggtg ctgcaaaagt caggcagccg caacgaaaaa caggtaaaag ttcgtggctt
      241 tgatagtcgg caggtgccgg tctatttcga cggtgtgccc atttatgttc cctatgacgg
      301 caacctcgat ctggcgcgga ttctgaccaa caatctgggg gcagtcgaag tttccaaagg
      361 gtattcgtcg ctgcttcatg ggcctaatca gatgggcgga gccattaata tcaccaccct
      421 gaagaagcaa gtctgggata tcgccaggga tggagccgta gtcaggacaa tgcctacgat
      481 atgcatgctt catttgccgc cagcagcgat cttgggtatt tgcaagtcag cggtagccag
      541 ctaaagcagg attttctcgg cctgccgcat ggtgtaaata atgatattgc aggcaaacac
      601 ggcaagatga ttaattcatc ggctgatgat aaacgcggca ttgtgaagct cggttttaca
      661 ccgcgcgaaa acgatgaata cacgctgact tacattaagc aggatggtga aaaagataac
      721 ccgccataca gcggaaatag tggtcaaaaa tcacgctact ggcagtggcc agagtatgac
      781 aaagaaagtt tttattatca gggaacgacc caactaaacg accgttttac cctgaaaagt
      841 cggctgtatc gcgacacctt tgaaaatacg ctgatgatgt acaactcgct ggctgatttg
      901 aaaaataaaa aaggcagcta cagccattat ttcgattaca gcgacggtgc cggattacaa
      961 ctggcagccg atgtgcgtga aaacgatctg ctgtcgtttg ccgttaactg gaaagatgac
     1021 gtacaccggg aaaaaggtgc gccgcacgcc gcttacgatc gctatgaaga tcgtacctgg
     1081 tcgctcgcca gtgaatatca atgggctgct gctgataatg tcgatgtcgt ggcgggaatc
     1141 agctatgact ggcgcgatag cgtagaagcg aaaaaacacg agaaagaggg cagcatcacc
     1201 cattatgacg acaacaatca gtcagctttt aactggcagg tgatggggaa ataccacttt
     1261 gccaatgaag acactctggc gctttcgtac tatgaccgca cacgctttcc gacgctgaaa
     1321 gaacgctata ccacatccaa acctgcgtat aaccagatag cgattgttaa cccgcagctc
     1381 aagccggaac gcgctcgcgg ggtggattta acctggaatg gtgccttcac gcgcgactgg
     1441 ggatttgagg tcagcgttta ctataaccgg gtgagtgatg ccatcctctc gcacaatatc
     1501 aatgccgata ccattcaaaa tcagaacagc ggcacggtgg attacagcgg tctggatgcc
     1561 ggtattaagg ggaaaatcag caatatactg gatgtaggat tgagctacgc tctgatccac
     1621 gctgacgcca aacgcaaaga catcggcaag ataaccgatc ttccaacgca gacaatgacc
     1681 gcatggatga cactcaaacc atgggagccg ttaagcgtaa cgctgtcgga agaggcgcgt
     1741 tcctccagct acagcaacag tgacggttca caaaaagccg ccggttttgc ggtgacccac
     1801 attcgagccg attacacctt aggtcatggc ttcagcgtca atgcgtcggt caataacctg
     1861 tttgataccc aatatgccta cagtgaaggg tttattgaag aaggtcgcaa tttctgggca
     1921 ggtgtcgagt atacgttctg atgtgaaagc ggatagcggc atctgtatcg ctatccgcct
     1981 tatgaaaatc ttattctctt cgcaggaggc ggaatacgcc cagcaccagg atagcgccca
     2041 ccaccgccac caggaagctg tgcaaattaa aaccgctgat ggagccgcca atgccaaaca
     2101 tggtcgccag ccagccgccg accaccgcac cgactatccc gagaatacag gtcaggaaaa
     2161 atccaccacc atcacgcccc ggcatgatta gcttggcgat aatgccggct atcaggccaa
     2221 aaataatcca ggcaataatt cccatgttca ggccctcgtt tcgatgaatc ggtcgcaaga
     2281 aatgcttacg tcaacgctct aagtataggc aagggcggga tcgatgctgg gcgtttgtcg
     2341 gctgatttaa gaacaacatg taaaaaatat tcacgcattt gtattaagtt ttgttaacta
     2401 tgaccgataa accaaagaca gtttgtcagt caggagtttt tccgcgtgag tcattaccat
     2461 gagcagttcc tgaaacaaaa tccgttagcc gtcctgggcg tgttacgcga tttgcacaaa
     2521 gccgcaattc ctttgcgtct cagttggaat ggcgggcagc tgatcagcaa aatactggca
     2581 ataaccccgg ataaactggt gctggatttc ggcagtcaag ccgaagacaa catcgccgtg
     2641 ctaaaggcac agcacattac cattaccgcc gaaactcagg gtgcgaaagt cgagtttact
     2701 gttgaacaac tacagcagag tgaatacttg cagcttccgg catttattac cgtaccgcct
     2761 cccaccttat ggtttgtaca acgacgccga tatttccgca tctccgcccc actccatccg
     2821 ccttattttt gccagaccaa actggcggat aacagtacgt tacgtttccg cttgtataat
     2881 ttgtcgttag gcggcatggg cgcattactg gaaacagcaa agcctgccga attacaagaa
     2941 ggtatgcgct tcgctcaaat tgaagtcaac atggggcaat ggggtgtttt tcactttgac
     3001 gcccagttaa tctccaccag cgagcgcaaa gtgattgatg gcaagaatga aaccatcacc
     3061 actccccgtc tgagcttccg ttttcttaac gtcagcccga cggtggagcg gcaattacag
     3121 cggattattt tctctctcga gcgagaagcc cgggaaaaag cggacaaagt gcgcgactga
     3181 tttacatcgc gtccagtgcc tgctcaagtt tgtagatata gcgcggagcc tgcggcgcag
     3241 gatgatttcg cgctacgtga tcgaggaact cgtcagcatc caaatcgttg attttgctga
     3301 tcgccttttt ccgatctgac gagaaagtcc gtagcagcgc acctgccccg ttagcgtatg
     3361 acaccaccag cgcatattgc agtaccttcg gatcttcaat gcctgccagc gggccggttt
     3421 ccagaatatt taggtaagcc gcccccattg aaatattacg ctctggattt ttcagctcgc
     3481 tggtcgtcgg ctcaccactc cagcccatac ggcgataaac atcacgtccg gaggttgaag
     3541 cttttaactg catcaaacca atggcattcg atttactcac cgcgttcgga ttaccacccg
     3601 attcgatagc gataatcgcc gtgatcaatt gtggatcgac gccccaggct gcaccggctt
     3661 tctggcttat tggcatccac tgcatcgcac gttgcaccgg aactttcgcg ttccacggcg
     3721 ggttcgtata gtcatgcttt gatgaacaac ccgctaataa cacaatcaaa aaggcaaacc
     3781 atctcaattt cacttcttct atccttatag cctggaatcg ccgttgaggt gacaacggca
     3841 cacctaagcg gcatgatatg catttcgttt ctgttgtcag caaggaattg ccatgtctct
     3901 gtttcactta attgccccat cgggttacag cattaaacag cacgccgcgc tgcgtggtat
     3961 ccagcgcctt accgacgcgg ggcatcaagt caataacgtc gaggttattg cccgtcgttg
     4021 tgagcgtttt gcgggtacgg aaacggagcg tctggaagat ctcaattccc ttgccagact
     4081 gactaccccc aacaccatcg tgctggctgt acgcggcggt tacggtgcca gtcgtttact
     4141 ggcagatatt gactggcagg cactggtggc tcgccaacaa catgacccgt tactcatttg
     4201 cggacatagc gattttaccg ccattcagtg cggtcttctg gcgcaaggca atgtcatcac
     4261 ctttagcggc cccatgctgg tagcaaactt tggtgcggat gaactgaacg cctttaccga
     4321 gcaccatttc tggctggcgt tacgcaatga aaccttcacc attgaatggc aaggggaagg
     4381 cccgacatgt cagaccgaag gcacgttgtg gggaggcaat cttgcgatgc tgatttcact
     4441 gattggtaca ccgtggatgc caaaaattga gaacggtatt ctggtgctgg aagatattaa
     4501 cgagcaccct ttccgcgtcg aacgtatgct gttgcagctc tatcatgcgg gaattttgcc
     4561 acgtcagaag gcgattattc tcggtagctt tagcggcagc acgcccaatg attatgacgc
     4621 aggttacaac ctggagtcag tgtacgcgtt tttgcgttcc cgcctgtcga ttccactcat
     4681 taccggcctc gattttggtc atgaacaacg cacggttacg ctgccgttgg gcgcacatgc
     4741 cattctgaat aacacccggg aaggcactca gttgacgatt agcggtcatc ctgttcttaa
     4801 aatgtaaaaa aatcacgctt cagggctaag taaaacgctg tctctgccgc taatatggta
     4861 gggtttatat aagagacagc gtaatcagga gtaaccgacc gttggatgcc acaacaataa
     4921 ttagcctttt catcttagga tccatccttg tcaccagcag tatattactt agttcatttt
     4981 cttcccgtct tggcattcct attctggtta tttttctggc gatcggcatg ctggcgggag
     5041 tcgatggcgt cggcggtatc ccgtttgata attacccctt cgcctacatg gtaagtaacc
     5101 tggcgctggc gattattttg ctcgacggcg ggatgcgcac tcaggccagt tcctttcgtg
     5161 tggcgttagg accggcgctg tcgctggcga cgctgggcgt acttatcacc tctggtttaa
     5221 ccggcatgat ggcggcgtgg ctgtttaatc ttgatttgat tgaaggctta ttaatcggcg
     5281 ctatcgtcgg ctccaccgat gctgcagcgg tcttttctct gctgggtggt aaggggctta
     5341 acgaacgtgt tggctcgacg ctggaaattg aatccggcag taatgatcca atggcggtct
     5401 ttctgacgat taccctgatt gcaatgatcc agcagcatga aagcagtgtc agttggatgt
     5461 tcgtggtcga tattctgcag caatttggcc tcgggattgt catcggtctt ggcggtggtt
     5521 atttactgct gcaaatgatt aatcgcatcg ccctgcctgc cggattatat ccattgctgg
     5581 cattaagcgg cggtatttta atttttgctt taaccacggc gctggaaggc agcggtattc
     5641 tggctgttta tctgtgcggt tttctgctgg gtaatcggcc gattcgcaac cgctacggca
     5701 tcctgcaaaa tttcgacggc ctcgcctggc tggcgcaaat cgccatgttc ctggtgctgg
     5761 ggctattggt taacccaagc gatctgctgc ctattgccat tccggcgctc attttgtcgg
     5821 catggatgat attcttcgcc cgtccccttt cggtatttgc tggattgctc cctttccgcg
     5881 gtttcaatct gcgtgagcgc gtgtttatca gctgggtggg attacgcggc gcggtgccaa
     5941 tcatcctggc cgtgttcccg atgatggcgg ggctggagaa tgcgcgactg ttctttaatg
     6001 tcgccttctt tgtggttctg gtttcactgc tcttgcaggg aacatcactc tcgtgggcgg
     6061 cgaaaaaagc caaagtggtg gttccgcccg tcggacgtcc ggtgtcacgc gttggcctgg
     6121 atattcatcc ggaaaatccg tgggagcagt ttgtttatca attgagtgct gataaatggt
     6181 gtgtaggtgc ggcactgcgc gatttgcata tgccaaaaga gacgcgtatt gcagccctgt
     6241 ttcgtgataa ccagttgctt catcctaccg gcagcacccg actgcgcgaa ggcgatgtat
     6301 tgtgtgtaat tggtcgggaa cgcgatctcc cggcgctcgg taaactgttc agccagtcgc
     6361 caccggtcgc gctggatcaa cgcttctttg gtgacttcat tctcgaagcc agcgccaaat
     6421 atgctgatgt tgcgctgata tatggcctgg aagacgggcg agaatatcgt gataaacagc
     6481 aaacgctggg tgaaattgtc cagcagttgc taggcgcggc accggttgtc ggtgaccagg
     6541 tagagtttgc cgggatgatc tggacggtgg ccgagaaaga agataatgaa gtgttgaaga
     6601 ttggcgttcg ggtggctgaa gaggaagctg aatcttaaat ttaatgcagc atctatcgcc
     6661 ggatgcgatg ctttactgaa cgcagcctga gcctgtaata aacttgtcat ttcctgattt
     6721 tgatatgctc atcccaacat cagaagaagg ttaaattatg gatgacaaac cattgcaggc
     6781 tctggctaat gaactcaaaa caccggctca agaaagtcag cgtaagggct ttttccgact
     6841 gtaacaggta ataaaaacca gccccgcatt accggtcaac catcaggggc ccctgcctga
     6901 catcgcctga acatattccc ccacgcaacc tgccgcactg acatagccca cacaataggc
     6961 gtttcatccg cctgtagcac cggatgcatg agcgatttct tatagttatt cctttgctta
     7021 ataacgatta tttccgggtc gttcactctc gtattttctt taatacgtca gagctaccta
     7081 ggccgtaata cagcatgtac cagacccgac acggcatttc tgaccaggga aaaagtcaac
     7141 gatgagtagt gatatcagcg caaatgacgt cctgtggctc ctttgcggtg accacattca
     7201 cgtctccggc gctgccagag gatatttatt tcaaggaaac gtagcattat ggctagcttt
     7261 aaagacatct gcctattcgc ccccgccatt cccccatctt ccgttaaccc aggcgtacga
     7321 cgcccgccag ggcggctctt cctgtcggtg gaactcaaga atcaaatttt gcagaacatg
     7381 aagtatctca gcccgcaaaa attcagctgg ggtgatgccc cctggcagat cattgacctc
     7441 agtatcgcgg gcaaagtgaa catacaggtg gacaacaata ccatcatcac gttg
//
LOCUS       DADLSB010000174         7491 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.174, whole genome shotgun sequence.
ACCESSION   DADLSB010000174 DADLSB010000000
VERSION     DADLSB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.174"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(76..357)
                     /locus_tag="JN331_003533"
     CDS             complement(76..357)
                     /locus_tag="JN331_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY7261571.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(619..1728)
                     /gene="apbC"
                     /locus_tag="JN331_003534"
     CDS             complement(619..1728)
                     /gene="apbC"
                     /locus_tag="JN331_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY7261572.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1860..3893
                     /gene="metG"
                     /locus_tag="JN331_003535"
     CDS             1860..3893
                     /gene="metG"
                     /locus_tag="JN331_003535"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY7261573.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERTEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4034..7491
                     /locus_tag="JN331_003536"
                     /pseudo
     CDS             4034..7491
                     /locus_tag="JN331_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
BASE COUNT         1944 a         1946 c         1808 g         1793 t
ORIGIN      
        1 tatcaataaa aaagacgaaa tcatttatat aaatgatttc gtctttctaa atgcaattaa
       61 aatcaggttg ccaaattaat cgtagcgatt taacacgatt cgacgtttgc tatcgctacc
      121 tgcaacaacc acttctgtcg taacaccttt gacgggcgaa gaaacctgat gacgggtgtc
      181 aacattatac gttaactgac cacaatgctg ttgtggttga ctattaacca tagccatact
      241 gcatgttggg ggactgatcc tgccattaat cgttaatgtg gcagtctctg aagagactgc
      301 gggagaagcc aatccatagg caagaaaaat aattcctgac agccaatact tattcattga
      361 acgttatccc tgtagtaaag gttatgcctg gatagaatga gtgcataaca aactatagct
      421 gtacatccac tacacagcac gaaggatgat aatgaagcat tgcctgtacg atcactcgac
      481 tttgtagagt ttcggacgaa ggtccgcaga atattcgcag tattaaataa gtgttcatta
      541 atgcggcaaa aacaaataca ccgcatcaat ataacatctc tgcggtgtat gaagaataac
      601 cagaatggtt aataggcgtt agaccgcgcg gaaggaaatc tcgcctggaa tgacttcacc
      661 ctgccagtag agctgagctg caacgcggtc agccagctgg cggtagatag cggtaaattc
      721 gctctccgga cggctgataa ccgttggcgt gcctttatcg agatcctcac gtaaggagat
      781 gtgtaacggc atctgcccca gcagctgtgt atggtatttt tcagccagtt tctctgcacc
      841 accggtaccg aaaatcggtt catggtgacc acagttactg caaatatgca cactcatgtt
      901 ctcgacaata ccgagcaccg gaacctcgac tttctcgaac atcacaatgc cttttttcgc
      961 atcgatcaac gcgatgtcct gcggggtggt gaccacgact gcaccagtta ctggaatgtt
     1021 ttgcgccagc gtcaattgaa tgtcaccggt gcctggcggc atatcaagca ccagatagtc
     1081 gagatccggc cacaaggttt cctgcaacat ctgcattaac gccttgctcg ccattggtcc
     1141 acgccacacc atagcattgt cgtcagtgac cagataacca attgaattgg ttgccaggcc
     1201 atgagacata atcggtgcca tgtgggtacc gtccggcgaa gttggacgct gattttctgc
     1261 gcccagcatg gtgggaattg atgggccgta gatatcggcg tccagaatgc cgactttcgc
     1321 cccttccgcc gccagtgcca gcgccaggtt taccgccgtg gacgatttac ccacgccgcc
     1381 tttaccggaa ctcaccgcga taatgttctt cacaccgtta attcccggct gattcttcac
     1441 gcgtttcaga gtggctatgt tatgcgacag tttccagtcg atagctttcg ccccggtgat
     1501 gcgcagcagt tcggcgctac attgctcttt taactcttcg aacgcgctat gccagacaaa
     1561 cggcataacc agttccacat gcaacgtgtc gtccatccag gcgacatggt gcaacgcttt
     1621 cagcgtggtg aggttatgct tcagggttgg gtgctgaaaa ttggccagtg tcccggcgac
     1681 cattgcgcgc aaggcttctg gtgatttggc ctgggattgt tcgttcatcc cgactccttt
     1741 tttgtataga taaaccatca gctgatagtt tacctgaaga atatagagaa gtacttactt
     1801 aacattttcc catttggtac tatctaaccc cttttcacta ttaagaagta atgcctacta
     1861 tgactcaagt cgcgaagaaa attctggtga cgtgcgcact gccgtacgct aacggctcaa
     1921 tccacctcgg ccatatgctg gagcacatcc aggctgatgt ctgggtccgt taccagcgaa
     1981 tgcgcggcca cgaggtcaac ttcatctgcg ccgacgatgc ccacggtaca ccgatcatgc
     2041 tgaaagctca gcagctcggt atcaccccgg agcagatgat tggcgaaatg agtcaggagc
     2101 atcagactga tttcgcaggc tttaacatca gctatgacaa ctatcactcg acgcacagcg
     2161 aagagaaccg ccagttgtca gaacttatct actctcgcct gaaagaaaac ggttttatta
     2221 aaaaccgcac catctctcag ctgtacgatc cggaaaaagg catgttcctg ccggaccgtt
     2281 ttgtgaaagg cacctgcccg aaatgtaaat ccccggatca atacggcgat aactgcgaag
     2341 tctgcggcgc gacctacagc ccgactgaat tgatcgagcc gaaatcggtg gtttctggtg
     2401 ctacaccagt gatgcgtgat tctgaacact tcttctttga tctgccctct ttcagcgaaa
     2461 tgctgcaggc atggacccgc agcggtgcgt tgcaggagca ggtggcaaac aaaatgcagg
     2521 agtggtttga atccggcctg caacagtggg atatctcccg cgacgcgcca tactttggtt
     2581 ttgaaattcc gaacgcgccg ggcaaatatt tctacgtctg gctggacgca ccgattggct
     2641 acatgggttc tttcaagaat ctgtgcgaca agcgcggcga cagcgtaagc ttcgatgaat
     2701 actggaagaa agactccacc gccgagctgt accacttcat cggtaaagat atcgtttact
     2761 tccacagcct gttctggcct gccatgctgg aaggcagcaa cttccgcaag ccgaccaacc
     2821 tgtttgttca tggctatgtg acggtgaacg gcgcgaagat gtccaagtct cgcggcacct
     2881 ttattaaagc cagcacctgg ctgaatcatt ttgacgctga cagcctgcgt tactactaca
     2941 ctgcgaaact ctcttcgcgc attgatgata tcgatctcaa cctggaagat ttcgttcagc
     3001 gtgtgaatgc cgatatcgtg aacaaagtgg ttaacctggc ctcccgtaat gcgggcttta
     3061 tcaacaagcg ttttgacggt gtgctggcaa gcgagctggc tgaccctgag ctgtacaaaa
     3121 cctttaccga tgccgctgaa gtgattggtg aagcgtggga aagccgtgaa tttggtaaag
     3181 cagtgcgcga aatcatggca ctggctgacc tggctaaccg ctatgtcgac gagcaggctc
     3241 cgtgggtagt cgcaaaacag gaaggtcgcg atgctgacct gcaggcgatt tgctcaatgg
     3301 gcatcaacct gttccgcgtg ctgatgactt acctgaagcc ggtactgccg aaactgaccg
     3361 agcgtacaga agccttcctc aatacggaac tgacctggga tggtatccag caaccgctgc
     3421 tgggccacaa agtgaatccg ttcaaggcgc tgtataaccg catcgatatg aaacaggttg
     3481 aagctctggt ggaagcctct aaagaagaag tgaaagccac taccgcgcca gttactggcc
     3541 cactggcaga tgatccgatt caggaaacca tcacctttga cgacttcgcc aaagttgacc
     3601 tgcgcgtggc gctgatcgaa aacgcagagt ttgttgaagg ttctgacaaa ctgctgcgcc
     3661 tgacgctgga tctcggcggt gaaaaacgca atgtcttctc cggtattcgt tctgcttacc
     3721 cggatccgca ggcactgatt ggtcgtcaca ccattatggt ggctaacctg gcaccacgta
     3781 aaatgcgctt cggtatctct gaaggcatgg tgatggctgc cggtcctggc gggaaagata
     3841 ttttcctgct aagcccggat gccggtgcta aaccgggtca tcaggtgaaa taatccccct
     3901 tcaaggcgct gcatcgacag cgccttttct ttataaattc ctaaagttgt tttcttgcga
     3961 ttttgtctct ctctaacccg tataaatact ggtagcatct gcattcaact ggataaaatt
     4021 acagggatgc agaatgagac actttatcta tcaggacgaa aaatcacata aattctggac
     4081 ggtggagcaa cagggaaacg agttgcatat cagttgggga aaagttggca ccaaagggca
     4141 aagtcagata aaaagttttt cagatgctgc ggcggcggaa aaagcggaac tgaagctgat
     4201 tgcggagaag gtgaagaagg ggtatgtgga gcaagcgaaa gataattctt tgcaaccttc
     4261 ccaaacggta acggactctc tcaaggtagc ggacttatcc accattattc aggaacaacc
     4321 ctctttcgta gcagaaaccc gtgcggctga caaaaataca gatgctgttt taccttggct
     4381 ggcgaaagat attgctgtcg tttttccgcc cgaagttgta cacaccacgt taagtcatcg
     4441 ccgctttccc ggagttcctg ttcagcaagc agacaaattg acccaattac gtcgcttagc
     4501 ctgtagcgtg tcgcaacggg ataataaaac agccacattt gacttcagca cctgttcttt
     4561 agaatggcaa aacaccgtcg cccaagcgat cagtcagatc gacggcctga aaacaacaca
     4621 gctaccatca ccagtaatgg ctgtactcac ggcacttgaa atgaaaccag gaaggcggtc
     4681 tggaatatgc tactgatgta ataatacacc ttcaacagat tgatattaaa tgggattatg
     4741 cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg gagcaatatt
     4801 ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct ctctggcaaa
     4861 aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg caaccattaa
     4921 ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag cgtctactgg
     4981 ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat gagcacattc
     5041 ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac tattgtggtg
     5101 cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc cgatttgctc
     5161 cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat ccgttcgcat
     5221 taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcga atgacgaaag
     5281 cctgcgctgc gttcccacac gcagcactgg cagcactggc ggaacttcta gtgcaaaaag
     5341 aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca gcactggcag
     5401 aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca tgccagcacc
     5461 aactgacaca gccctcaaac cataccagcg ccgatctact gccagccata gtagtctccc
     5521 caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta gcgccattag
     5581 gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg gcgaaatata
     5641 tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac ctgttggcaa
     5701 ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa gcggtagttg
     5761 aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg tttcattcac
     5821 ctaccgggca ttattgacag ttggggattt tgacaacatt gccgctggag gctggagaaa
     5881 gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga atacgccatg
     5941 ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg ctatccgcag
     6001 gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc cgtcgcccgt
     6061 gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt gaaatacccg
     6121 gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga agcacaggat
     6181 aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt actgcaagaa
     6241 atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct gcttgcgctc
     6301 gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta tcagccatcg
     6361 atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa cgccctcctc
     6421 cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg attattgcag
     6481 gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctaggatct gtttaccgcc
     6541 tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt aggcgttctc
     6601 ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc tggtgaatcc
     6661 cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag tgatatcgcc
     6721 ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca ggagcgggca
     6781 aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga gcttgaagat
     6841 cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga ttttggccca
     6901 cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga tgtttccggc
     6961 agccgcctga aagacctgcc aaaaccaaac aaaagcgatg atgagtcaca ggcgaacgat
     7021 gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgccgc ccagcaggta
     7081 gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa cttccaactc
     7141 ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg gggcgtttat
     7201 agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag ctacagcacc
     7261 gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat tcctcacgtt
     7321 ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga ctacgaactg
     7381 ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc cgagcgcaac
     7441 gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt c
//
LOCUS       DADLSB010000175         7446 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.175, whole genome shotgun sequence.
ACCESSION   DADLSB010000175 DADLSB010000000
VERSION     DADLSB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.175"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="JN331_003537"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="JN331_003537"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY7261574.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JN331_003538"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JN331_003538"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY7261575.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLRMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JN331_003539"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JN331_003539"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY7261576.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILLSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /locus_tag="JN331_003540"
     CDS             complement(3025..3480)
                     /locus_tag="JN331_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY7261577.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JN331_003541"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JN331_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY7261578.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JN331_003542"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JN331_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY7261579.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="JN331_003543"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="JN331_003543"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY7261580.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQSLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAGILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1704 a         1913 c         2087 g         1742 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacgcaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gacagaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggaactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgct cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactctgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaagtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cactgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 ggcattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccg
//
LOCUS       DADLSB010000176         7405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.176, whole genome shotgun sequence.
ACCESSION   DADLSB010000176 DADLSB010000000
VERSION     DADLSB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.176"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..267
                     /locus_tag="JN331_003544"
     CDS             <1..267
                     /locus_tag="JN331_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7261581.1"
                     /translation="GDVLYPVKITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRL
                     VIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(343..807)
                     /locus_tag="JN331_003545"
     CDS             complement(343..807)
                     /locus_tag="JN331_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7261582.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAQGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEADYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(804..1679)
                     /locus_tag="JN331_003546"
     CDS             complement(804..1679)
                     /locus_tag="JN331_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY7261583.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1676..2365)
                     /locus_tag="JN331_003547"
     CDS             complement(1676..2365)
                     /locus_tag="JN331_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY7261584.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2413..3903)
                     /gene="nanT"
                     /locus_tag="JN331_003548"
     CDS             complement(2413..3903)
                     /gene="nanT"
                     /locus_tag="JN331_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY7261585.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKDKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4013..4906)
                     /gene="nanA"
                     /locus_tag="JN331_003549"
     CDS             complement(4013..4906)
                     /gene="nanA"
                     /locus_tag="JN331_003549"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY7261586.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5028..5819)
                     /gene="nanR"
                     /locus_tag="JN331_003550"
     CDS             complement(5028..5819)
                     /gene="nanR"
                     /locus_tag="JN331_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY7261587.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            6199..>7405
                     /gene="dcuC"
                     /locus_tag="JN331_003551"
     CDS             6199..>7405
                     /gene="dcuC"
                     /locus_tag="JN331_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7261588.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHF"
BASE COUNT         1819 a         1966 c         1870 g         1750 t
ORIGIN      
        1 ggtgatgtgt tgtatccagt aaaaataacc agaaaatcat caggaaaaac tgccttccac
       61 cttgttccat tcggcttaaa taagactcac gaccttctag aggtggaaga tgcttcagaa
      121 gcaattcggc tggtgattga tgaacgtcat tccattcgct gcagtacact tacagcaact
      181 attacgaata aaaagggcaa gcgaattaag cggacaggta tatacagtat caagggtgtt
      241 aacataaaag aatacaacgt caggtaatgg cacagagcgg tccgtcagtt caggtttggc
      301 tctgtgccaa agatgggtca ggtcacacct gatctaataa atttaagcca ttaaatcagc
      361 cttaaccttc accaccacct ttttaatctc acccggctcg ccaacaacgc atcccggctt
      421 atgcggctct cctggcataa acacggcgaa cattcccggt ttcaggatga tggtttgctc
      481 gttttcaatg gcgctacaaa gctggtaatc agcttcatgg tggaactctt cacattggcg
      541 cgcagtgcct gccatgccaa acagaatccg ttcctcaccg tttaataaca gctggatatc
      601 aatgtattgc tcgtgcaatt ccgctttttt ctcgacgggc gattgagtgt taaacgtcat
      661 gacattcata aaaatattat cgccctgtaa ttcgtaacga ccctgcgctt tttcttgcgg
      721 tctggcagct aaggccagcg ttaacgcgtc ctgtaacgca ggatgtaacc cagcagacgg
      781 taatgactgt acttcaccca tcatcataat ttttctccct gggccaacag cgcagcccca
      841 agtaaacctg catcatggcg gtaatgcgcc gccagtaaat caacatgaaa tgccgctggc
      901 tcctgcgcca gatacatttc caccaacgcc agataccctt ctgccagacc aacgctgcca
      961 ccaaccacca cgcactggca atcagttgtg gctttaatat cagcgatcag ccttgcaagc
     1021 gtacgtgcgg agcgatgaat cagttgctgc gcctgctcat cgccctgccc ggcgcgtgtg
     1081 aaaatagttc tcgcatccgc gccagccagc tcgccctgcg ctgccgccgc aatgccgcga
     1141 ccagaagcaa ttgcttccac gcaacctgtg cgtccacagc cgcagactgg gccgtgtgga
     1201 tcggcaagcg tatgcccgat gtgccccgcc agaccgccag ggccggtaag cagtttgccg
     1261 ccgctcacta caccgccgcc gacgccagtg gaaacggtga taaagaccat atcggttata
     1321 tcgccttcca gcgcctgata ctctgcccat gctgcggcct gcgcgtcgtt aatggcaatg
     1381 gtcggcaaat tggtaagttg ttccagcgtt ttgactaacg gaaagtgtag caatccaccg
     1441 agattatgcg gattcagcgc cagcaaactg ccgtcgcgaa taattccggt tgaagcgatg
     1501 gcaacccgct gcgcatgagc ttgcaacgga gagactaatg cggataaggc atcacgcaag
     1561 gcttctggtg tctggctggc tggcgtagga agttcacgac gatcgcggat ctgcccgtca
     1621 gcgccaatca gcgcggcggc aagtttagta ccgccgatat caatcgccag tatggtcata
     1681 gcaccgcctt tttcatcgct gtgttgtacc actgacaaat gtgttcaaga cgcgtaatgg
     1741 cagaaccgac cgtcaccgcc cacgcgccgt ggcgcatcgc atctgccgcc tgagcaggcg
     1801 tgttgtaacg cccttcggca atcacccgac atccggcttc gctcaacgtt ttcaccagcg
     1861 ccagatccgg ctcttctggc gtttcaggcg tggtatagcc agaaagcgta gtgccaataa
     1921 tttcggctcc cagcttttgg catgccaggc cgtcttccgg cgttgagcag tcggtcatcg
     1981 ccagtaaacc gtgatgatgg atacgtgcca gcagcgtttc aacaggcacc ggacgcgggc
     2041 ggtcggtgcc gtcaatggcg ataatgtccg cgcccgcctg cgccagcgca tcgacatctt
     2101 caatataggc cgtgatgcgt accggagaat cctccagatc gcgtttcaca attccaataa
     2161 tcggcacgct caccaccgca cgcgtggctt gcagatttgc cacaccttcg atgcgaatgg
     2221 caaccgcgcc cgcctgctct gccgctaatg ccatggctgc gacgatttcg ggtttatcga
     2281 gcgggctgtc aggaaccggc tggcaggaga caatcaggcc accgttagca gcgatttttt
     2341 gatccagttg tgcaagtaac gacatacatc ttcccttagc gaaaggcccg gtacatagac
     2401 cgggcaacag gattaacttt tggttttgac taaatcgttt ttggcgctgc caaatggcac
     2461 ggcaccgcta aatggtttgc cgtcgatagc gtcatgagta cgcaatgctt ccgggcgcag
     2521 ccaacgctga acgcgggaag gcatatccag accaatcagc aggatcacca caaatgtcag
     2581 gctgaacgag agcgatgcca gcgcagtacc cagatccaga cgttgagcga tcaacgcgcc
     2641 gatgattggg gccagtgcgc cacccaatgc gccaacgttg taggtaaagc ccaggcccgc
     2701 tgcacgctgg tcggtatcga aataaccgcc aatcagtttc ggtaagatcc cggcgatccc
     2761 ttgtccaagc atttgctgga agaacagtaa cagaccgagc acccagacgt ttgcgccgcc
     2821 aatcgcaaat accggaataa tcagcagctg cgaggccagc aggctacaaa cgtacgcttt
     2881 gcgggttccc agccagtcac cgaggaagcc acctacgcag catcccacgg ccgcgccaaa
     2941 gccactaaag aacagcacat tggcgacagt atgcgggtta taagccagat cggttttcag
     3001 atacgttggc agcagcgcct gaatcggcca tgagtagagg aaagcaaaca acacgaccac
     3061 catcagcatt acgcccgttg gccagcgttt acccgtactt tgcaccataa agctgataaa
     3121 gattgcggcg cataacagcc cgagaacagc gacgattgca gcattttgca ggttaccggc
     3181 gaagcagaac cacagcgcag tagccgccgc cagtgtcatt acgatattgg caatgcgatg
     3241 ttcaccacgg tagagaatat ccaccattgt gcgtactggt gctttacctg cgtgtttatc
     3301 tttccagtct tccgcttccg ggatgttttt acgcagccag agagcaaaga tgattggcaa
     3361 aatgccgata aagaacagcg cacgccaacc ccagaccgga accaccaggc tatagacctg
     3421 agcggcaacg acggccccca cagagaagcc tgaaatcaaa aaaccactgg ctttgttacg
     3481 cagatgtttt ggccagcttt caatgacata ggtggcgctg gaaccgtatt cacccgccat
     3541 ccccatgccg atgaccagac gagcgataaa catggtgatg tagcctggcg caaagccgca
     3601 ggccagcgtc ccggccgaga agagaacgat gctggtgacc attgccagac gacgcccgta
     3661 gcggtcaccc atagcgccga gcatcaggcc gccgaaccag cgagagataa aggctgcgga
     3721 gatcagactt gccgcctgca ccgtcgtcag cccgaattca ccctgtactt cggtgagtac
     3781 cagggcgatt aaaacgaaat caaaaccgtc aagcagatat cccaaccagg cagcggaaaa
     3841 tgcgcgccat tgtgcacggt tgagatggcg ataccacggg atattctggg ttgtagtact
     3901 cattgtgagt ctcccgcggt gggcgatgcc cacacgcttt ggtatgaaaa ttgtagggta
     3961 cagatgcgtt tatttcccct cacccggtag gggcgagcga ggggaaaaca actcacccgc
     4021 gctcttgcat caactgctgg gccagcgcct tcagttctgg cagatatttt tcatctaccg
     4081 gtccaaacgg tttgcggcac agcggcacag aaacgacatc catataatgc aggacagttt
     4141 tcaggccgcg gaatacgccc gttttgatca gtaaatcaat gactttgttg cactcagttt
     4201 gcagtttctg cgcggtctgg atatcgcctt ctttcagcgc cttcacgatc ccctgatatc
     4261 gccagcccat gatgttgtaa gtactgccga taccaccatc agcgcctgcc agcaagccgg
     4321 aagcgaagat ttcgtcgtaa ccgttataga gcaccagatc aggatgttca cgacggatct
     4381 gttccatctg atagagatcg ccagaagtct gtttcagcgc accgacgcca ggcaacgtaa
     4441 ccagggtgtt gatctgatcc agggtcagtt ttaccccact cagggctgga atgttgtaca
     4501 ccaccatcgg caaaccatcc gctgaatcaa taattgcccg atagtgatcg cagtgttctt
     4561 caaagctgaa aggatagtag aacggcgtga cggcagagac ggcatcgaag ccataacgtt
     4621 tagccgatgc cgcaagttgt tggctttcgg cggtgctgac gcaaccgacg tgggcgatga
     4681 gtttaatctt acctttcgcc tcttcggcga cgatttccag tacctgttca cgctcggaaa
     4741 ggctttgtac aaaggcttcg ccggtcgaac cacccacgta taaaccgtcg atgccctgct
     4801 gaatattgaa ctgaaccagg cggcgcagac tcgctttatc cagtgcttgt tgttgatcaa
     4861 aaggagtcag gagtgcagcc attacgccac gtaaattcgt tgccataaat acctctgaag
     4921 tgatgcttgt ctgataaacg atataccttt atacctgtta taccagatca attaagcaac
     4981 accccataca gaaagcttat aatgcgatct gcttcactaa agtggcatta tttctttttg
     5041 ttggtggtct gaccgaaagc gtgccaggta gcagagacgc tgttgagatg cgattgcaac
     5101 gcacgatcgg cttcgtcagg atcatgacgg cggatcgcat caacgatcgc aatatgctgt
     5161 tgataactaa cgttgttatg ttcgtgcagt gcctgatcgg taaccgttgg gcgtgcggca
     5221 ataagccagt cgagcagggc aacgtggatc gccatgaaga ttgggttacc ggggatctcc
     5281 gccagcacgc ggtggaaatc aacgtctgaa cgaatgaatg ccgcgttgtt atccagcgac
     5341 tgactgttga tttccagtgc ttttgccagc aaatcgattt gctcatcggt ggcgtgttca
     5401 gccgcatagc gcaccaggct ggattcaaag aacagacgta attgttcgaa atgggcaatc
     5461 ccaccaggat gagaaaggaa atctttcgcc atgccggaaa gctcgccgat gatggtgtcc
     5521 gcagaaggtc gagagacacg agcgcgttcg ccgttgttta tttgcaccag acctttgcgt
     5581 tttaacgctg ccagcgcttc acgcacagaa ggacgcccga cgttaaagaa cgccatcagt
     5641 tcgcgttcag acggtaattg ttcaccttcg ccaaattcac gacggcggat catctgttcc
     5701 agctcttctt ccaccatttc ggagagtttt ttacgcgcca gcgggcggct acgcaagttg
     5761 cgaccaattg caggtgaaga atcttcggtt tgcgaatcaa atgcgttcat aaggcccatt
     5821 ctgtaaggtc agtgtgatta acatcatcag tgacatccta tcacaggatt gaaagtaggg
     5881 gaaaatggca ggtttttctc tttgtgcctc atcattacca taattaacgg aataattaac
     5941 tattgcgaaa aattaatgta acgcagataa acacatcccg attgaattat tcataagact
     6001 attcacaagc attatgaata ttatgaatgt gttcttacaa aataatcata atcgcatatt
     6061 ttttaatgaa aaatcacctc acctacaatt aaaaacacga tatccgcacc ataaatagac
     6121 ttgcaaaaaa tataacatca ttgttttcaa tctgccgttt atgggattga ccgttttctt
     6181 ttgacacgga gttcaacaat gttcggcata attatatctg tcatcgtatt aattacgatg
     6241 ggctatttga tcctgaaaaa ctacaaacct caggtagtgc tggctgccgc aggtgtcttc
     6301 ctgatgatgt gcggagtctg gttagggttc ggtggtgtac tcgatcccgc caaaagcagc
     6361 ggctacttga tcgtcgacat ttataatgaa atcctgcgca tgctgtccaa ccgcattgcc
     6421 ggattggggc tgtcgattat ggcggtgggc ggttatgccc gctacatgga gcgcatagga
     6481 gccagtcgcg cgatggtgag cttgctaagc cgcccgttaa aactcattcg ctcgccgtat
     6541 attattctgt cggcaactta cgtcatcggc caaatcatgg cgcagtttat taccagcgcc
     6601 tccggtctgg gtatgttgct gatggtcacc ttatttccga cgctggtgag tctgggagta
     6661 agtcgcctct ctgcggtggc ggttatcgca accacgatgt ccattgagtg ggggattctg
     6721 gaaacgaact ccatttttgc agcccaggtc gcgggaatga aaattgccac atacttcttc
     6781 cactaccagc ttccggtcgc ctcttgcgtc attatctcgg tggcgatctc ccactttttc
     6841 gtgcaacgcg cttttgacaa aaaagataaa aatatcaatc acgaacaggc agagcaaaaa
     6901 actctcgata atgtcccgcc gctctattac gccattttac ctgtgatgcc gttaatcctg
     6961 atgctcggct cgctgttcct cgcccacgtc gggctgatga agtcagaact gcatctggtg
     7021 gtggtgatgt tactaagttt gacggtgacg atgtttgttg agttcttccg caagcataac
     7081 ttgcgcgaaa caatggacga tgtgcaggcg ttttttgacg gcatgggtac gcagtttgtc
     7141 aacgtggtaa cgctggtggt cgcgggtgaa atatttgcga aaggcttaac gacgattggc
     7201 actgttgatg cggttatcag gggtgcggag cattctggtc tgggcggtat tggcgtgatg
     7261 attattatgg cgctagtcat tgccatttgt gccattgtga tgggctctgg caatgcgccg
     7321 tttatgtcat ttgccagtct tattccgaat atcgcagccg gactacatgt accagcggtt
     7381 gtaatgatta tgccgatgca ttttg
//
LOCUS       DADLSB010000177         7311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.177, whole genome shotgun sequence.
ACCESSION   DADLSB010000177 DADLSB010000000
VERSION     DADLSB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.177"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..457
                     /locus_tag="JN331_003552"
     CDS             <1..457
                     /locus_tag="JN331_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7261589.1"
                     /translation="FLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAF
                     PFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVAS
                     VVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            559..2013
                     /gene="amn"
                     /locus_tag="JN331_003553"
     CDS             559..2013
                     /gene="amn"
                     /locus_tag="JN331_003553"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY7261590.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2356..3072
                     /locus_tag="JN331_003554"
     CDS             2356..3072
                     /locus_tag="JN331_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7261591.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3528..3603)
                     /locus_tag="JN331_003555"
     tRNA            complement(3528..3603)
                     /locus_tag="JN331_003555"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3568..3570),aa:Asn,seq:gtt)
     gene            complement(3704..5347)
                     /gene="yeeO"
                     /locus_tag="JN331_003556"
     CDS             complement(3704..5347)
                     /gene="yeeO"
                     /locus_tag="JN331_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HAY7261592.1"
                     /translation="MLRYILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVQGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5352..5427
                     /locus_tag="JN331_003557"
     tRNA            5352..5427
                     /locus_tag="JN331_003557"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5385..5387,aa:Asn,seq:gtt)
     gene            complement(5465..6415)
                     /gene="cbl"
                     /locus_tag="JN331_003558"
     CDS             complement(5465..6415)
                     /gene="cbl"
                     /locus_tag="JN331_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY7261593.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFARKGLPV
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6517..>7311)
                     /gene="nac"
                     /locus_tag="JN331_003559"
     CDS             complement(6517..>7311)
                     /gene="nac"
                     /locus_tag="JN331_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY7261594.1"
                     /translation="ELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNV
                     GQSLSGQVSIGFAPGTAASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLD
                     MAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLR
                     VDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITT
                     PSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS"
BASE COUNT         2059 a         1756 c         1588 g         1908 t
ORIGIN      
        1 tttccttaat attggtttgc tggtaggtgg attaagctgc ctgacaattc cctgttttgc
       61 ctggcttgcc gatcgttttg gtcggcgcag ggtttatatc acaggcgcgt tgatcggaac
      121 gttgagcgca tttcctttct ttatggcgct tgaagcacaa tctattttct ggatagtttt
      181 cttctccata atgctggcaa acattgcgca tgacatggtg gtgtgtgtgc aacaaccgat
      241 gtttaccgaa atgtttggtg ccagttatcg ctacagtggt gctggagtcg gttatcaggt
      301 tgccagtgtg gttggcggtg gatttacacc ttttattgcc gctgcactca tcacttactt
      361 tgccgggaac tggcatagcg tcgccattta tttgctggct ggatgcctga tttctgcaat
      421 gaccgcttta ttgatgaaag acaatcaacg ctcgtgatag cctggcgaag atcatccgat
      481 cttcgcctta tacttttgtt tcacattcct gtgacatact atcggatgtg cggtaattgt
      541 atggaacagg agacacacat gaataataag ggctccggtc tgaccccagc tcaggcactg
      601 gataaactcg acgcgctgta tgagcaatct gtagttgctt tacgcaacgc cattggcaaa
      661 tatattacaa ttggcgaatt acctgatgaa aacgcccgta aacaaggtct ttttgtctat
      721 ccatcactga ccgtaacctg ggacggtagc acaacaaatc cccccaaaac gcgcgcattt
      781 ggtcgtttta ctcacgcagg cagctacacc accacgatta ctcgccctac tctctttcgt
      841 tcgtatctga atgagcaact aacgttgctt tatcaggatt atggtgcgca tatctctgtg
      901 caaccctcgc aacatgaaat cccttatcct tatgtcatcg atggctctga attgacactt
      961 gatcgctcaa tgagcgctga tttaactcgc tacttcccga caacagaact ggcgcaaatc
     1021 ggcgatgaaa ctgcagacgg catttatcat ccaacggaat tctccccgct atcgcatttt
     1081 gatgcgcgcc gcgtcgattt ttccctcgca cggttgcgcc attataccgg tacgccagta
     1141 gaacattttc aaccgttcgt cttgtttacc aactacacac gttatgtgga tgaattcgtt
     1201 cgttggggat gcagccagat cctcgatcct gatagtccct acattgccct ttcttgtgct
     1261 ggcgggaact ggatcactgc cgaaaccgaa gcgccagaag aagccatttc cgaccttgca
     1321 tggaaaaaac accagatgcc agcatggcat ttaattaccg ctgatggtca gggtattact
     1381 ctggtgaata ttggcgtggg accgtccaat gctaaaacca tctgcgatca tctggcagtg
     1441 ctacgcccgg atgtctggtt gatgattggt cactgtggcg gattacgtga aagccaggcc
     1501 attggagatt atgtccttgc acacgcttat ttacgcgatg accacgttct tgatgcggtt
     1561 ctgccgcccg atatacctat tccgagcatt gctgaagtgc aacgtgcgct ttatgacgct
     1621 accaagttag tcagcggcag gcctggtgag gaagtcaaac agcggctgcg tacaggtact
     1681 gtggtaacca cagatgacag gaactgggag ttacgttact cagcttctgc acttcgtttt
     1741 aacttaagcc gggccgtagc aattgatatg gaaagtgcaa ccattgccgc gcaaggatat
     1801 cgtttccgcg tgccatacgg gacactactg tgtgtttcag ataaaccgtt acatggtgag
     1861 attaagcttc ccggccaggc taaccgtttt tatgaaggcg ctatttccga acacctgcaa
     1921 attggcattc gggcgatcga tttgctgcgc gcagaaggcg accgactgca ttcacgtaaa
     1981 ttacgaacct ttaatgagcc gccgttccga taataagtta tgaaccttct tatcagaaat
     2041 agatgtgaag gattaagtaa gacatctggc aaatagcctg caataaacgc gggctgcttg
     2101 cttccttggg cagatgcgag ttttattatc gtcttaatga tttccacata ttaaaagcaa
     2161 gtatgctttc aaaacacaat tataaaaaat cccgccaaca acattagttt ttataaaatt
     2221 aaatataaga ttatggcttt agaatatttt tatttctaat agacgagatt tttcctgtta
     2281 tgatataata tgccgaatta acacatgtta acgatttacc agtaatgtaa ataaattttc
     2341 gaggagatca ttccagtggg acgtaaatgg gccaatattg ttgctaaaaa aacggctaaa
     2401 gacggtgcaa cgtctaaaat ttatgcaaaa ttcggtgtag aaatctatgc tgctgccaaa
     2461 caaggtgaac ccgatccaga attaaacaca tctttaaaat tcgttattga acgtgcaaag
     2521 caggcacaag ttccaaagca cgttattgat aaagcaattg ataaagccaa aggcggcgga
     2581 gatgaaacgt tcgtacaggg acgttatgaa ggctttggtc ctaatggctc aatgattatt
     2641 gctgagacat tgacttcaaa tgttaaccgt acgattgcta acgttcgcac aattttcaat
     2701 aaaaaaggcg gcaatatcgg agcggcaggt tctgtcagct atatgtttga caatacgggt
     2761 gtgattgtat ttaaaggaac agaccctgac catatttttg aaattttgct tgaagctgaa
     2821 gttgatgttc gtgatgtaac tgaagaagaa ggtaatattg ttatttatac tgaacctact
     2881 gaccttcata aaggaatcgc ggcgctaaaa gcagctggaa tcactgagtt ctcaacaaca
     2941 gaattagaaa tgattgctca atctgaagtt gagctttccc cagaagattt agaaatcttt
     3001 gaagggcttg ttgatgccct tgaagatgac gacgatgtac aaaaagttta tcataacgtc
     3061 gcaaatctct aattatattt taaagaaatc tgtctttatg gcagatttct ttaatctcat
     3121 ataattctta taaaaaatat aatgtttaac tcgtcatatt gcttataaaa aatccccgtt
     3181 cccagagaaa taatatttat taaaatggca ggtcttcttt ttctgattac cgaagacaaa
     3241 gtggcaatta cgtatagttt cccgataaag acgcgacagc tacatcccgc ataaggcatt
     3301 tttctcttta tctttgtacg gtacttcatg gaacagaatt tttgaccttg tgaatcgtga
     3361 tgtctgttgg ggaggggcaa tttgctcact gaagcgtgag actcgattaa gcgcacgaaa
     3421 cacagaaatc aaaaaacccg gccacttttt tacaaagtgg ccgggtaaaa ataattttta
     3481 ttttttaact gtcctgagtc tcatagagat gtctcaaaac taaaatttgg ctcctctgac
     3541 tggactcgaa ccagtgacat acggattaac agtccgccgt tctaccgact gaactacaga
     3601 ggaatcgtga gaacgaggcg aatattagcg atgcccaccc acaatgtcaa agcctgtttt
     3661 ttaaatttga aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacaactg
     3721 gctttttttc acacttttgc ggctcggatc gagggtattt ccacagccaa cgtccagtaa
     3781 ccattcgcca gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga
     3841 caccaaccac accccagcca agcatgattc cctgcacata accgactaca acccggcaac
     3901 cccacatgct caacatagaa acccacatgg cgtaacgggc atcacgagca cccttaaatc
     3961 cagcgggtag cacccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca
     4021 gaatcacaac gacgtgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg
     4081 caaagggagc cgttagccag gcgatggccg ttaatccaag agtggacagc cagaacacat
     4141 gccgcaactg aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga
     4201 tcgtagaagc agaaccgagc gcacttccgg gtaagttgat aagagccgca attgagaacg
     4261 cgataaaatt tccggcaata acgctggtcc ccatcccggc aacgaacatt tgggttaata
     4321 accgaccact ggtaaataac actgattcga cactcgctgg aataccaatc cccatgactt
     4381 cccagataat gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag
     4441 gattaaaacc aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg
     4501 ttaaacccaa cccagccccg acaaatccca gtcccggcca ggagaaaagg ccgtaaatca
     4561 atatgccgct aataataata ttaagaatat tcaggctacc gttaatcagt agcggtattt
     4621 tcgtattccc tgcaccacga agtgccccgc taccaataag agtgatggct gcggctggat
     4681 aactgagtac ggtcagctcc agataagtca acgccagtgc tttaacttct gtcgtggcat
     4741 cacccgcgac gaaatcaata atttgttcgc caaaatgatg aattagcgtt gccaacagta
     4801 cggcaaacaa cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat
     4861 cccgcttacc gagactaaat gctacaacga cagtagtacc aagatcgata gctgcaaaaa
     4921 aagccataat gaccatattg aagctgtccg ccaaccccac gccggccatc gcatcttttc
     4981 ccagccagct gaccagaaaa gtgctcagga cccccatcaa caggacacag gcgttctcca
     5041 tgaagatagg aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc
     5101 gtttagagtg ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag
     5161 ccgaccttaa ttgcagaaag tgaaaccaca tttcaaatga tgagggagaa tcagcaaagc
     5221 tgcaaagatt ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca
     5281 aacagttggg aaatttaaaa atcgggtttg acaaaatatt tttcgccgtt aagatgtacc
     5341 tcaacagcga ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca
     5401 ctggttcgag tccagtcaga ggagccaaat ttctgaaaag cccgctttta tagcgggctt
     5461 tttgctatat ctgataatca atttcctctt cactgttttc catcacctgg cgcttgatat
     5521 cctcaactga cagtcctgca ttacaaagtt ccagaaagcg ccaaacatag ttacgctgaa
     5581 gttgtcctcg cttcagtccc aaccagacag tattagcatc gaaaagatgc cgcgtatcca
     5641 ggcggattaa attctcttcc tcttgttcgc cgctggattg ttcggcaact aatccgatcc
     5701 caagcccaag agcaacatag gttttaatga catcagaatc ctgtgcactt aatacaatat
     5761 ctaccggcaa acctttgcgg gcaaatgcgt catcagtgcg tgagcgcccc gtaattccct
     5821 gtcggtaagt aattaacggc cacttcgcta ttgattccag cgtcaatggt gtaatttgcg
     5881 ttaagggatg atcgagtgga acaagcaaac tatggtgcca acaaaaccac gggaaggcga
     5941 cgagctgcgg gtcattactc aaacgctcgc tggcgatacc aatatcagct tcgccatttt
     6001 gcaacaacgt cgcaatttcc tgtggcgaac cctggattag ctcgagccga acctccggga
     6061 aaagttcgcg aaaagcttta atgacctctg gcaagctata acgtgcctga gtatgcgtcg
     6121 ttgcaatagt gagaacacca gacgtatcgt tggtaaacag gtctgcaagc cgacgaacat
     6181 tactggcttc attcagaata cgttctgcaa tgaccagtaa tgctttgccc ggttcagtca
     6241 tgcccagcag tcgcttacct cgtcgaacaa atatttcgat gccaagttca tcctccagtt
     6301 cccgaatatg acggctaacg cctgactgtg aggtaaaaag catattcgca acctctgtca
     6361 ggttgtaatc ctgacgtgca gcctcgcgga ttatctttag ttgttggaaa ttcacggtaa
     6421 actccgggca gttcagatat cccgttattg ttaaagtcta atgcccggca taacaaataa
     6481 taaaaacccg catcttattc catcccgata taacgcttag ctcaccaatt gccactgcct
     6541 tttttccatc actggagaac taatcactga cattaacaac tctttcactg cctgtgcctg
     6601 tggtgacaag tttgctctgg cgggtaaatt taaggacaaa gagagactca ttgaaggagt
     6661 ggtaatgcgt gacatccacc catttactgc gccacataac gaacgcgcag ccgattcagg
     6721 taatactgca acgcccatgc cgctggcaat cgctgcggta agcgtggcaa tagactcaat
     6781 ttcaccaatg acttttgccg tgagccgccg tagggaaaaa gcctcatcaa cacgaagtct
     6841 aacagcgctg taatcactgg ggagaaaaag gttcatttgc gcaatagcat taacatcaac
     6901 gttttgcccc gggcaatctt gagttcctac caggaaaaga tcttctttca gcaaagcctg
     6961 actggatacc ccagccacag gggaatgctc ataaatcacc gccatatcga gttggtgatt
     7021 tatcagtttt tcgttaagca ctgcaccact attttcatga agatagataa cgacctccgg
     7081 gaattcagcg cgaaccgcct gtaataaggg catggtgatg gatgacgcag cggttcctgg
     7141 cgcaaagcca atcgagactt gccccgataa tgactgacca acgttatgca ccgccagttg
     7201 ggcctgctca cactgacgta aaatggcccg tgcatgggta tagagaattt ttccggcgtc
     7261 tgttggcgta acgccccgtt ttgtgcgaat caaaagctgt tgatttaact c
//
LOCUS       DADLSB010000178         7309 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.178, whole genome shotgun sequence.
ACCESSION   DADLSB010000178 DADLSB010000000
VERSION     DADLSB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.178"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            459..2048
                     /gene="purH"
                     /locus_tag="JN331_003560"
     CDS             459..2048
                     /gene="purH"
                     /locus_tag="JN331_003560"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY7261595.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            2060..3349
                     /gene="purD"
                     /locus_tag="JN331_003561"
     CDS             2060..3349
                     /gene="purD"
                     /locus_tag="JN331_003561"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY7261596.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(3346..4671)
                     /gene="zraR"
                     /locus_tag="JN331_003562"
     CDS             complement(3346..4671)
                     /gene="zraR"
                     /locus_tag="JN331_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY7261597.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCATLNESLLESELFGHEKGAF
                     AGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQVMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(4668..6044)
                     /gene="zraS"
                     /locus_tag="JN331_003563"
     CDS             complement(4668..6044)
                     /gene="zraS"
                     /locus_tag="JN331_003563"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY7261598.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            6282..6707
                     /gene="zraP"
                     /locus_tag="JN331_003564"
     CDS             6282..6707
                     /gene="zraP"
                     /locus_tag="JN331_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY7261599.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(6709..>7309)
                     /locus_tag="JN331_003565"
     CDS             complement(6709..>7309)
                     /locus_tag="JN331_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY7261600.1"
                     /translation="HNSSLPPFTASGFAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGD
                     TTTREYRWQGDNLTLININVYSKPPVNIRARFDDRGDLSFMQRESDGEKQQLSNDQID
                     LYRYRADQIRQISDALRQGRVVLRQGRWHAMEQTVTTCEGQTIKPDLDSQAIAHIERR
                     QSRSSVDVSVAWLEAPEGSQLLLVANSDFCRWQPNEKTF"
BASE COUNT         1672 a         2041 c         1967 g         1629 t
ORIGIN      
        1 tttagcgcac tgtcgcgagg tggcgtatat tacgctttcc tctttcagag tcaaccctga
       61 atttcaggat ttttctcttc aaccgaaccg gctgtttgtg tgaagtgatt cacatccgcc
      121 gtgtcgatga aggcacatta tagggagttc tccgcaggcc gcaatagaaa aattgcagaa
      181 aaatgactga ctgctgcatt ccccagcaaa accccgcttt ataccttttt acgcacagag
      241 ttatccacaa tcatcaatgt aatttctgta tttttcccac ggtaaccact gtcaaaattg
      301 tgatcaccat tgaaagagaa aaattcgcga gcgttgcgca aacgttttcg ttacaatgcg
      361 ggcgaaaaat aaggatgccc cgttaggggc gttagctgag tttttcgcga aaaattcagc
      421 taacgctctc tgtaatagtc aaacccaggg gatttaccat gcaacaacgt cgtccagtcc
      481 gccgcgctct gctcagtgtt tctgacaaag ccggtatcgt cgaattcgcc caggcacttt
      541 ccgcacgcgg tgtggagctg ctgtctacag ggggcactgc ccgtctgtta gcagaaaaag
      601 gtctgccggt aaccgaagtt tccgattaca ccggtttccc ggagatgatg gatggacgcg
      661 tgaagaccct gcatccgaaa gtacatggtg gcattctggg ccgtcgcggc caggacgatg
      721 ccattatgga agaacatcag atccagccta tcgatatggt ggttgttaac ctgtatccgt
      781 tcgcccagac tgttgctcgt gaaggctgct cgctggaaga tgcggttgag aacatcgata
      841 tcggcggccc gaccatggtg cgctccgccg ctaagaacca taaagatgtc gcaatcgtgg
      901 tgaagagcag cgactacgac gccattatta aagagatgga tgacaacgaa ggatcgctga
      961 cgcttgcaac ccgtttcgac ctcgccatca aagccttcga acacactgcc gcctacgaca
     1021 gcatgattgc caactacttc ggcagcatgg ttccggcgta ccacggtgaa agcaaagaag
     1081 ccgccggtcg cttcccacgc acgctgaacc tgaacttcat taagaagcag gatatgcgtt
     1141 acggcgagaa cagccaccag caggctgcct tctatataga agagaatgtg aaagaagcct
     1201 ccgttgctac cgcaacccag gttcagggta aagccctctc ttataacaac atcgccgata
     1261 ccgatgcggc gctggagtgc gtgaaagagt tcgccgagcc ggcatgtgtg attgtgaagc
     1321 acgccaaccc ttgcggtgta gctatcggca attccattct tgatgcttac gatcgcgcgt
     1381 acaaaaccga cccaacctcc gcattcggcg gcatcattgc ctttaaccgc gagctggatg
     1441 cggaaaccgc acaggccatc atttctcgtc agtttgtcga agtgattatt gcgccttccg
     1501 ccagcgaaga agccctgaaa atcaccgccg ccaagcagaa cgtacgcgtt ctgacctgcg
     1561 gtcagtgggg cgagcgtgtt ccgggtcttg atttcaaacg cgtgaacggc ggtctgctgg
     1621 ttcaggatcg tgacctgggg atggtcggtg cggaagaact gcgcgtcgtc acccaacgtc
     1681 agccgaccga acaggaactg cgtgatgcgc tgttctgctg gaaagtggcg aaattcgtga
     1741 aatccaatgc tatcgtctat gccaaaaaca atatgaccat cggtattggc gcgggccaga
     1801 tgagccgcgt gtactccgcg aaaatcgccg gtattaaagc tgccgatgaa ggcctggaag
     1861 tgaaaggttc ctcgatggct tctgacgcgt tcttcccgtt ccgcgacggt attgatgccg
     1921 ccgccgctgc gggcgtgacc tgcgtaatcc agcctggcgg ttccatccgt gatgacgaag
     1981 tgattgccgc cgccgacgag cacggtattg cgatgctctt caccgacatg cgccacttcc
     2041 gccattaatg gagcaataga tgaaagtatt agtgattggt aacggcgggc gcgagcacgc
     2101 gctggcctgg aaagcggccc agtcgccgct ggttgagact gtttttgttg ctccgggtaa
     2161 tgcaggcact gcgctggaac ccgcgctgca aaacgttgct atcggcgtga ccgatatccc
     2221 ggcgctgttg gatttcgcac aaaacgaaaa gattgatctg accatcgtcg gcccggaagc
     2281 gccgctggtg aaaggcgtgg tcgatacctt ccgcgccgcc gggctgaaaa tcttcggccc
     2341 aaccgcaggt gcggcccaac tggaaggctc aaaagcgttt accaaagatt tcctggcccg
     2401 ccataagatc cctacggcgg aataccagaa cttcaccgag gtagaacctg cgctggtgta
     2461 tctgcgtgag aaaggcgcgc caatcgtcat taaagcggac ggtctggctg ccgggaaagg
     2521 cgttatcgtg gcgatgacgc tggaagaagc ggaagcggct gttcacgata tgctggcggg
     2581 caacgctttt ggcgacgcgg gtcatcgcat cgttatcgaa gagttcctcg atggcgaaga
     2641 agcgagcttt atcgtgatgg tggacggcga gcatgtgctg ccgatggcta ccagccagga
     2701 tcacaaacgc gtaggcgata aagataacgg accaaacacc ggcgggatgg gcgcttactc
     2761 ccccgcgccg gtagtaaccg atgaagttca tcagcgcacc atggaacgta tcatctggcc
     2821 aaccgtgaaa ggcatggcgg cggaaggcaa cacctacacc ggttttctct atgcgggcct
     2881 gatgatcgac aaacagggca atccgaaggt tattgaattt aactgccgct ttggcgatcc
     2941 ggaaacccag ccgattatgc tgcgcatgaa gtccgatctg gttgagctct gcctggcggc
     3001 ctgtgaaggc aaactggacg agaaaacgtc agagtgggat gaacgtgctt ctctcagcgt
     3061 ggtgatggct gcgggtggat atccgggcga ttaccgcacc ggcgatgtga tccacggcct
     3121 gccgctggaa gaagtggctg acggcaaagt gttccacgcg ggcacaaaac tggcggatga
     3181 cgagcaggtg gtaaccagcg gcgggcgcgt actgtgcgtc accgcgctgg gtcataccgt
     3241 ggcagaagcg cagaaacgcg cctacgcctt aatgaccgat atccactggg atgattgctt
     3301 ctgccggaaa gatatcggtt ggcgcgccat cgaacgcgag cagaactaac gcgacagttt
     3361 tgccaatagc gttttgcgcg tgatccctaa ctgacgggcg gcttcggttt tgttgccgcc
     3421 cgttttctcc agcgccgcca gaatcacctc tttttccact tccaccagcg gctgaatatc
     3481 ctgactttgc cccagcggga tcggcgtact ggcaatcgcc agcggcagct cgcgttcgga
     3541 aatatattcc ccggtcagca gcaccaccgc ccgttccacc gcgttttcca gttcacgaat
     3601 atttcccggc cagtcgtagt ggatcagcaa atccatcacc tgtggcgtaa agccttttac
     3661 cgccttgcga ttacgctcgg caaagcgctg aagaaaatgg ccagccagca gagggatatc
     3721 ttcccgccgt tgccgcagcg atggtacttc aatcgccacc acattcaggc gatagtagag
     3781 atcctggcga aaacgcccgg cattcacctc tgcggcaaga tcgcgatggg tcgccgcaat
     3841 cagccggaca tcaaccgaga tagtctggtt gctaccgaca cgctgaactt cgcgctcctg
     3901 aatcgcacgc agcagacgca cctgcatcat cggcgagata tcgccaattt catcgagaaa
     3961 cagcgtgccg ccgtccgcct caacaaagcg cccttcccgg cgtttatcgg ctccagcaaa
     4021 cgcccctttt tcgtgaccga acaattcaga ttccagcaag gattcgttga gtgtcgcaca
     4081 gttgagcgtt accagcggtt tttcgctacg tgcgctactg gcgtgaatcg ccctagcgac
     4141 cagttcttta ccggtgccgg aatcgccgtg gatcagcacc gtggcttccg atggcgcgac
     4201 gagggcgatt tcactgagca ggtgttgcat cgccgggctt ttaccgacca taccgaactg
     4261 gctggcagtc accgcaggtg tttcagcatc aatactgtgc gtatgcgcga gcgccttttc
     4321 cagcgtcgcc tgtaggttat cgaaatccag cggcttgatg agataatcca gcgccccagt
     4381 tttcagtgcc tctaccgccg tctcgacgct ggagtacgca gtcataatca gcaccggaat
     4441 tgccgggttt aacgctttga tctctttcag cgtggcgatg ccgtccatct ccgccattcg
     4501 cacatcgcaa agcacaagat caaaaacctg ttcccgcacc tgctccagcg cctgtcgccc
     4561 gctgttcgcc agcgcgacgt tatagcccca gccgcgcagt aaagcctgca aaatagtgca
     4621 gtggctaatg tcatcatcca ccaccagaat atcgatatta tcgtgcgtca tccttgtggg
     4681 tccttacgcg taatattgac cggaagccag agggtgaacg ttgagccttt tccctcctgg
     4741 cttgcgacct gaattgtacc accgtgttgt tcaacaatat tatgcacgac cgccagcccc
     4801 aatccggtgc cttcggcttt ggtggtgaag tacggagtga agatggcttc aagctgatct
     4861 gccgcaattc ccttaccgct gtcggtaacg ctgattttca cgcccgcgcc gctttcgctg
     4921 gccgtcacgc taatcacgcc atgctgacca atcgcctgaa tagcattgag atagagattc
     4981 aacaggacct gagtcagcct gtccggatcg gcctgaattt ccggtaatgt gtcgttggcg
     5041 gtaaagcgta actggatctc ccggctgttt gcatcctggc ttaccagctg taatgagtgg
     5101 ttaatcagcg tgttgagatc caccgcctgc aaagccagat gcgttggctt aaccagttcc
     5161 agcaactcgc ttaccacgcg gtttaaacgg tcggcctctt tcgccatcac ctgcgccagt
     5221 tgatgcgctt ctccccctgc aggcgcgcgc tcggcaaagt atttcgccag tcctttaatc
     5281 gaggaaagtg ggttacggat ttcgtgggca acgcctgccg caagatgccc cagcgccacc
     5341 agcttctctt tgcgcttcat ttcatcttgt agaagctggc gcgagcgcag atagcggcga
     5401 taccagaaga atgacaatac gcttgccagc aagaccgtcg ccagggcgaa gagaataatc
     5461 agggtgtttc tctgctcacg atcttcggca ctcacaatat tactggcgtc gaaagcgatg
     5521 aaaattgctt gtggtgtcgc ggcatactgc tgcatatggc gcatccggtg cattccagca
     5581 gcaaacatcg gttgaaactg gcgataaatt tccagaacag gctcgccgtc tgcgctatcg
     5641 atccgccgcc acgcttcttc atctcccgga tgtaactgct gcatttcctg aggggaataa
     5701 agctgttttc ccaccatgcc ggagttgcta tgcatcacga ttgttccttg ttcatccgtg
     5761 accgcaaacc aacgtactcc aggctgcccg gccatttctt ccagtaaggc ctgctgctgc
     5821 gcatgatgca tgcgcatccc catgccgacg cgcgagccgg attcaagagc gcgaataagt
     5881 acactgcctt tttccagcag cgtttgtctg gcggcggcag tctcgcgccc ataatcgcga
     5941 atcaccgtca ccgcaaacaa ccctaccagc ccaacaatga ccacggggag gatcgcgctt
     6001 aaccatttag ctaaggagtc tttagaacgt tgcataaaac gcatcttctt ctttgcctgc
     6061 tcatcccttt ctgacagcag aaatcatgcc atcttttatc agcgcttacc ctgcgctgta
     6121 acacaaaggc ttaagtttca atgagtaaaa atgactcgct acccgcagca ggcgagtcat
     6181 ttttactcgt ttatcatgcc agattacccg tcatatcagc gtttcatcgt tggcacggaa
     6241 gatgcaatac ccgaagtaag acaaccactg gaggattaac catgaaacgg aacacgaaaa
     6301 ttgccctggt aatgatggcg ctttcagcaa tggcgatggg atcgacatct gcatttgctc
     6361 acggcggaca cggtatgtgg cagcaaaatg ccgcgccttt gaccagcgaa caacagacag
     6421 cgtggcagaa aatccataat gacttttacg ctcaaagcag cgcactgcaa cagcaactgg
     6481 tgacgaagcg ttatgaatac aatgccctgt tagccgcgaa cccaccggat agcagcaaaa
     6541 ttaacgcggt cgccaaagag atggagaatt tgcgtcagtc gttagatgag ttacgggtga
     6601 aacgagatat cgcgatggct gaagcgggta ttccgcgcgg agccggaatg ggcatgggct
     6661 acggcggctg cggtggtggc ggtcatatgg gtatgggcca ctggtaaatc agaacgtttt
     6721 ctcgttgggt tgccagcgac agaaatcaga gtttgccact aacagtaatt gcgaaccttc
     6781 gggcgcttcc agccatgcca cgctgacatc aacagaagag cggctctggc ggcgctcgat
     6841 atgcgctatc gcctgcgaat ctaaatcagg tttaatggtt tgcccttcgc aggtggtcac
     6901 ggtctgttcc atcgcatgcc agcgcccctg gcgcagcacg actctcccct gacgtaaggc
     6961 atcgctaatc tggcggatct gatcagcacg ataacggtat aaatcgattt ggtcgttaga
     7021 aagctgctgc ttttccccat cggattcacg ttgcataaag ctcagatcgc cgcgatcgtc
     7081 aaaacgcgcg cgaatgttca ccggcggttt gctgtaaaca ttgatattga tgagcgtgag
     7141 gttatcgccc tgccagcgat actctcgcgt cgtggtatcg ccactgcgcc acgggctaaa
     7201 cacggcaagc agatgcacat tatcgccgct gtcttttcgc cagatgcgta ccgcgccctg
     7261 gtcttcagca aatccactgg cggtaaaggg aggaagtgaa gagttgtgg
//
LOCUS       DADLSB010000179         7252 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.179, whole genome shotgun sequence.
ACCESSION   DADLSB010000179 DADLSB010000000
VERSION     DADLSB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.179"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..186)
                     /locus_tag="JN331_003566"
     CDS             complement(<1..186)
                     /locus_tag="JN331_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261601.1"
                     /translation="MLQSSADKACNVLVKKVRYIKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAF"
     gene            264..1358
                     /locus_tag="JN331_003567"
     CDS             264..1358
                     /locus_tag="JN331_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HAY7261602.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1382..1594)
                     /locus_tag="JN331_003568"
     CDS             complement(1382..1594)
                     /locus_tag="JN331_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY7261603.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1854..2162
                     /locus_tag="JN331_003569"
     CDS             1854..2162
                     /locus_tag="JN331_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY7261604.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2221..3315)
                     /gene="yaiW"
                     /locus_tag="JN331_003570"
     CDS             complement(2221..3315)
                     /gene="yaiW"
                     /locus_tag="JN331_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY7261605.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPSLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3328..4548)
                     /gene="sbmA"
                     /locus_tag="JN331_003571"
     CDS             complement(3328..4548)
                     /gene="sbmA"
                     /locus_tag="JN331_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY7261606.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4900..6057
                     /locus_tag="JN331_003572"
     CDS             4900..6057
                     /locus_tag="JN331_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-12 family class D beta-lactamase AmpH"
                     /protein_id="HAY7261607.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6058..6561)
                     /locus_tag="JN331_003573"
     CDS             complement(6058..6561)
                     /locus_tag="JN331_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7261608.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6649..7176)
                     /locus_tag="JN331_003574"
                     /pseudo
     CDS             complement(6649..7176)
                     /locus_tag="JN331_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1841 a         1849 c         1747 g         1815 t
ORIGIN      
        1 aaaagctatg aaatatattc tcttcgttaa cttataatta agagaaaaaa actcaccgca
       61 ttgccattta agccaggaat aaattagctt cccgttcatc atttttgtga tcttgcgcac
      121 atctttgata taacgcactt tcttcaccag tacgttgcac gctttgtcag cagaagattg
      181 caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt
      241 gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta
      301 aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat gccattgata
      361 aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg
      421 atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg cgcccttcgg
      481 cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac gcgcaaaacg
      541 gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag
      601 atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc
      661 tggcttcagc agcctgtatg gataaagatg tcaccaaacg tctgctacgc gatgccgggc
      721 tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc agttttgccg
      781 aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag ggctcttctg
      841 ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat ctggcgttcg
      901 agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc gaatgcgcag
      961 ttctgggcaa cgacaatccg caagccagca cctgtggcga gatcgtactc accagcgatt
     1021 tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag
     1081 ccattgcacc agagatcaac gataagatcc gggcgattgc cgttcaggct tatcagacgt
     1141 tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga
     1201 tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg aagctgtggc
     1261 aagccagcgg tctgggttac accgatctga tcacacgttt gattgagctg gcgctggagc
     1321 gtcacgctgc ggataacgca ttgaaaacca caatgtaata ttttaacgcc cgataatatt
     1381 cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaaccct gccagccaga
     1441 aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt gcttcaaatc
     1501 ccagtacccc caccacgcca ttaagaatga atataaggcc aatggcgaac gcatagtagt
     1561 gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg aaaaacaaca
     1621 tccttgcata ttctgtaaca accgtctgtg gtgtagacaa cattttcgta attgttaata
     1681 aatttaactt aaggttgcca ttttgtgact cacaacgaag attcacgctt cgggaatatt
     1741 cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt cagacttgcc
     1801 ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt tttatggctg
     1861 atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg
     1921 gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg caattgctga
     1981 acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag gaaacaggtc
     2041 gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc ccgtttttag
     2101 caggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat cgccgccagt
     2161 aaaatttacg ttgccagatg cggcctgaaa cgccttatct ggcctgacat tcctgtttga
     2221 tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt
     2281 caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt cgcggggtag
     2341 tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtatagcg ccgtctcctc
     2401 aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc tcatccccag
     2461 ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac tgtcgtaacg
     2521 aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgactg cgttctgaaa
     2581 cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt atatcggtgc
     2641 gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc cgccgcggcg
     2701 actgaagact tcctgacgaa ctgtaccgtc cattttccac ggataccctt tggtatgctg
     2761 ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcaccggat taagcgaacc
     2821 aaacagcgtc tgccccattg gtaccatgct gatcagatcg tcaaaaatcg cgctcaattg
     2881 cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg gcgatttgat
     2941 ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat
     3001 ctcttgccag gcgatcttgc ttaagctcgg aacggccgga tctgcctgat aatttgactc
     3061 ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca gcccctgact
     3121 ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat
     3181 cttctgccgc acgacgctcg ctacatctat cgctttttcg ccctttttca acggttgcgg
     3241 agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg aagatagatg
     3301 attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt cctgaatttt
     3361 gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca tcaactcaac
     3421 cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt
     3481 ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg gaaacagcaa
     3541 gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt tgaaatacat
     3601 atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg ctaaacagct cgcgtaccgt
     3661 aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac ggtaggcggc
     3721 ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca ctgccagcaa
     3781 tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg gaatgtgccc
     3841 gataatcggc agctccggca catgcgcgga gagcgttacc agcaccggca ggaaggcgat
     3901 caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg aagcaaaacg
     3961 catggtgtct tcctgcacac gctgtgcggc accttcgata tgacgcagtt gttgccagtt
     4021 cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acgtagtgac tgacaaagaa
     4081 gttgttcagt acactgatca ccaccgcgat cagcgcaatc cccagaaaga cgcccacttc
     4141 gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt gaatcagatc
     4201 atagaacggc gcataccagg cgttgacggc gaccccgact tccaccaaaa accaagtgac
     4261 gaagataatc agcgcagtac cgagtattga ccagtattgc caacggtgcg ggctgtagat
     4321 aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa
     4381 atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc cggtgatacg
     4441 cgccacccag tcacccccac cggcttgcca gaagataacg gcaatcaatg cccaaacaaa
     4501 ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac ttaaacatat gcactcctgc
     4561 taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta caaagcgtca
     4621 gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca gcctgaccat
     4681 aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg attaacacgc
     4741 gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac gcgccgttac
     4801 gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga gtagtataaa
     4861 tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag tctgcttttt
     4921 tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc gatcactgaa
     4981 ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta cggcagcggt
     5041 gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg tagttatggc
     5101 gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg tattgcttcc
     5161 ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg gaccgtaaag
     5221 ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccgac ctacaatggg
     5281 acgcccatta ccctagtgaa tctggcaacc cataccagcg ccctgccccg tgaacagccc
     5341 ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgagcaacg ctggaaatac
     5401 ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc taaccttgcg
     5461 ttcgacctgc tggcagatgc actggcgaat gcgtccggca agccttatac ccagttgttt
     5521 gaagagcaga ttacccgtcc attggggatg aaagacacca cctacacccc ctcaccggat
     5581 cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg
     5641 gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg gatgcagcag
     5701 tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca gacgttgatt
     5761 tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa agcggatgcg
     5821 cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat tattcagaag
     5881 acaggcggtg gtggcggttt cattacctat atggcgatga tcccgcagaa gaatattggc
     5941 gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag cgatggcatc
     6001 aatgatctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc atcctgatta
     6061 atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt cgatatagcc
     6121 gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc gtgtacgttg
     6181 atggatataa ttcatcacac caatacgtga tcgcaattct tcattccagt caatcattga
     6241 taataatgtt gcgcgggttt gtttggctgg tagatgatat cccgtacaat ttccttctga
     6301 tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca taatataagg
     6361 agcctgggta ataccgataa gtacgttttc ttctctacgc agagagataa ctccttctaa
     6421 aataacgaaa gtatcttcgt tattgacatc actggaaaat ataacttgct tttcattatt
     6481 aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa attcctgaag
     6541 aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca aatccttgac
     6601 gggcattatt tagattaaat taccagtatt tcttcggagt gaagaatatt accaggtata
     6661 tttaacaccc acgttcgcgg accagtcttg atctacgtca ccaccaccga ggtagttagc
     6721 atcggtatag gcgctgaagt tcttggtgaa gctgaactga gtacccagac caacacgtac
     6781 cgcagaccct tcagtaccgt tatcgatgga atcaccgttc acatcgttat cgttgttaga
     6841 gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga gcctgatctt cgctgtaggt
     6901 gaaggtataa cctgcatcta cacccagttc ataacgcatg ctgtcgtaag actgaccgtc
     6961 aactttcatg tcgttgctca gctggtagtc atcaccagac tggaacagac cagaaatgct
     7021 gccgtaagga gtcacgtaac cagcatcacc cagtttgaag tcgtaaccgg ctttcaaacc
     7081 gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa gtaccgttgc tcatggttgc
     7141 agacaggtcg ttgttgaagt gagagtagct caagctatga atcgccacgg ataatctaga
     7201 cacttccgag ccgttgataa tactggtttt catattctgt cggtgacatc tg
//
LOCUS       DADLSB010000180         7131 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.180, whole genome shotgun sequence.
ACCESSION   DADLSB010000180 DADLSB010000000
VERSION     DADLSB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.180"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(32..1672)
                     /gene="pgm"
                     /locus_tag="JN331_003575"
     CDS             complement(32..1672)
                     /gene="pgm"
                     /locus_tag="JN331_003575"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY7261609.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLSFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(1698..2243)
                     /gene="seqA"
                     /locus_tag="JN331_003576"
     CDS             complement(1698..2243)
                     /gene="seqA"
                     /locus_tag="JN331_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY7261610.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            2428..3192
                     /gene="ybfF"
                     /locus_tag="JN331_003577"
     CDS             2428..3192
                     /gene="ybfF"
                     /locus_tag="JN331_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7261611.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            3263..3625
                     /gene="ybfE"
                     /locus_tag="JN331_003578"
     CDS             3263..3625
                     /gene="ybfE"
                     /locus_tag="JN331_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY7261612.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            3765..4295
                     /gene="fldA"
                     /locus_tag="JN331_003579"
     CDS             3765..4295
                     /gene="fldA"
                     /locus_tag="JN331_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY7261613.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            4505..4591
                     /gene="uof"
                     /locus_tag="JN331_003580"
     CDS             4505..4591
                     /gene="uof"
                     /locus_tag="JN331_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY7261614.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            4584..5030
                     /gene="fur"
                     /locus_tag="JN331_003581"
     CDS             4584..5030
                     /gene="fur"
                     /locus_tag="JN331_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY7261615.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(5114..5440)
                     /locus_tag="JN331_003582"
     CDS             complement(5114..5440)
                     /locus_tag="JN331_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261616.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(5477..6883)
                     /gene="chiP"
                     /locus_tag="JN331_003583"
                     /pseudo
     CDS             complement(5477..6883)
                     /gene="chiP"
                     /locus_tag="JN331_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         1732 a         1822 c         1774 g         1803 t
ORIGIN      
        1 cttgcgaccg cccttttttt attaaatgtg tttacgcgtt tttcagaact tcgctaacaa
       61 tctcaaccgc ttctttctca atctgcttgc gatgttcttc accgaggaag ctttcgcagt
      121 agatcttata tgcgtcttcc gtgcctgacg gacgcgcggc gaaccagccg ttgtcagtca
      181 tcactttcag accgccaata gaagcaccgt tgcccggagc agcagtcagg cgcgcggtga
      241 tcgggtcacc tgccagggtg ctggcgctca ccatttccgg agacagctta gacagcgccg
      301 ctttttgtgc ggaagtcgca gctgcctgca aacggttgta gctcggcgca ccaaagcgtt
      361 ttgccagttc gttgtagtgt tcctgcgggt tcttaccggt gacagcggtg atttccgccg
      421 ccagcagaca cataatgatg ccgtctttgt cggtggacca cggcgtgccg tcgaaacgca
      481 ggaaggaagc ccctgcgctc tcttcgccgc caaagccgaa gctgccgtcg aacagaccat
      541 caacaaacca tttgaaacct accggtactt ctaccagctt acggcccaag tcattgacca
      601 cacggtcgat catcgcagaa gaaaccagcg ttttaccgac agcaacatct ttgccccact
      661 gcggacgatg ctggaacagg tagttgatcg ccaccgccag gtagtggttc ggattcatca
      721 aacctgccgg agtgacaata ccgtgacggt cataatccgg gtcgttagca aacgacagat
      781 cgaacttatc acgcagtgcc agcagacccg ccatcgcaca ctcggaggag cagtccatac
      841 ggatcgcacc gtctttatcg aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa
      901 cgatggtcag gttgaggttg taatactcgc caatgcgctt ccagtattcg ataccggaac
      961 cgcccaacgg atcaacgccc agcgtcaggc ccgctttctg aatcgccgcc atatcaacga
     1021 tatcggccag cccttccacg aacggctgca ccagatcctg ctctttcaca tggccggatg
     1081 ccatcgcttc gtcgagggag atacgcttca cgcctttcag gccatcggcc atcagtgcgt
     1141 tggccctgtc ttccaccact ttggtgacgt tagtatcagc cgggccacca tttggcggat
     1201 tgtacttgat accaccatct tccggcgggt tatgggacgg tgtaatcacg ataccgtctg
     1261 ccagcgggcc acctttttta ttgtgaacca ggatggcatt ggaaacggca ggcgtcggag
     1321 tgaagccatt gttttcctgc acaatgacat caacgccgtt cgctgccagc acttccagta
     1381 cggaaatgaa cgcaggctcg gacagggcgt gagtatcttt acccacatag caagggccag
     1441 tgatgccgtt tttcgcacgt tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct
     1501 cgttaaagct gtggcgcgct gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt
     1561 gctccgcatt ccctgcttct ggtttcagta cataatattg cgccgtcagt tgggcgacgt
     1621 taatcaaatc actctgttgt gcaggttggc ctgcacgatt gtggattgcc attgctttgt
     1681 cctttgtctg caacgtttta gatagttccg caaaccttct caatcaattc cgccgggaat
     1741 tgcatcgact gcatgatgtg ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg
     1801 atcacccaat acggcgtgcc tggcacatgt ttcggcttgg tctgattacc atttttcagc
     1861 agcgtttgtt catctgccgc aaagtacacg cgtgtacgac cgtgcaacga ttccgttgct
     1921 tcggcaaacg cctgggcgtc aagagaatat agtgtagaca acagcagcat aaagcgattg
     1981 accgctcgct tttgctctgc gtattcgtcc gaaagcagaa gttcacgcat tgcgcgaacc
     2041 ttgtctttaa tcgttttgac cggcttcgct tcgacgatag caggtgacgc aacgcgaacc
     2101 tctttcgtca ccggagcagc aggctgtgat gcggcggaaa atttcaacat acgccgtaaa
     2161 atgtcggatg cgctctcgcc gatatgctta gtgtggctgg caatatagct gtagagttca
     2221 tcatcaactt caatcgtttt catcttaatc cagtgcagtg tcttagctga atacaagtcg
     2281 ccaagagtat atgaataaat cccggcagcg gatagcgtca agcctggctt gcagaaaaac
     2341 tcggaataaa ccttatttgt tgcagccctg tggcacaatg gtcaacataa taccctaacc
     2401 cgacaacgcg aaaaaaagaa ctttgccatg aaattaaata tccgcgcgca aactgcacaa
     2461 aaccagcaca ataattctcc catcgttctt gtccatggtc tgtttggtag cctcgacaac
     2521 cttggcgtac tggctcgcga tctggtaaac gatcacaata tcatccaggt tgatatgcgt
     2581 aaccacggtc tttcaccgag agagccggta atgaattacc cggcgatggc gcaggatctt
     2641 gttgataccc tggatgcact gcagatcgac aaagcaacat ttatcggtca ctccatgggc
     2701 ggtaaagcgg taatggcact tactgcactg gcccccgatc gcatcgataa actggtggcg
     2761 atcgatatcg caccggtcga ctatcacgta cgccgtcatg atgagatttt cgcagctatc
     2821 aacgcggtca gtgaatcgga cgcacaaact cgccagcaag cagcagcaat aatgcgccag
     2881 catcttaatg aagaaggggt gattcagttt ctgttgaaat cttttgttga cggggagtgg
     2941 cgctttaacg tgccggtatt gtgggatcag tatccgcata ttgtgggttg ggagaaaatc
     3001 ccggcatggg atcaccctgc cctgtttatc cctggcggca attctccgta tgttagcgag
     3061 cagtaccgtg atgatttact ggcacaattt ccacaggcac gggcgcatgt gattgcaggc
     3121 gcgggtcact gggtccatgc tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat
     3181 ctcaatgatt aacgcaactg attaaaaacc cagcgtccgc gtcgctttcc gcgcgtgggc
     3241 gttggcgtga ctcatcgcgc tgatgtatta tggcgcgcta tccatccggg cagaggcctg
     3301 gctgattgtt tcccccgaag tcaccaagat catggccaaa gaacaaacgg accgtacgac
     3361 attagatctg ttcgcgcacg agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg
     3421 cgatgaacag ctgcgtatta ataaacgcaa ccagctaaaa cgcgacaaag tacgtggcct
     3481 taagcgtgtc gaactgaagc tgaacgcgga agctgtcgag gcgctgaacg agctggcgga
     3541 atcgcgtaat atgagccgta gcgaactgat cgaagagatg ttaatgcagc aactggcggc
     3601 gctgcgtagt cagggcatcg tttaaatttc cactttcatg tagcacagtg tgcagtcctg
     3661 ctcgtttgct gattccgcaa gactgcctgt tctgctatga ttgcctttat ccgtgggcaa
     3721 ttttccaccc ccatttcaat aagtttcaag aggttatttc actcatggct atcactggca
     3781 tctttttcgg cagcgacacc ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc
     3841 ttggtaaaga cgttgccgat gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag
     3901 cttacgacat tctgctgctg ggcatcccaa cctggtatta cggcgaagcg cagtgtgact
     3961 gggatgactt cttcccgact ctcgaagaga ttgatttcaa tggtaaactg gttgcgctgt
     4021 ttggttgtgg tgaccaggaa gattacgccg aatatttctg cgacgcattg ggcaccatcc
     4081 gcgacatcat tgaaccgcgc ggtgcaacca tcgttggtca ctggccaact gcgggctatc
     4141 atttcgaagc atcaaaaggt ctggcagatg acgaccactt tgtcggtctg gctatcgacg
     4201 aagaccgtca gccggaactg accgctgaac gtgtagaaaa atgggttaaa cagatttctg
     4261 aagagttgca tctcgacgaa attcttaatg cctgatgtga tgcggcgtag actcatgtct
     4321 acgccgtatt aatagataat gccaatcaaa ataattgcta caaatttgta acttttgctg
     4381 ttgtacctgt acaatgtccc agtgttcaag tggccttgcc gttgtaaatg taagctgtgc
     4441 cacgttttta ttaacaatat ttgccaggga cttgtggttt tcatttaggc gtggcaattc
     4501 tataatgata cgcattatct caagagcaaa ttctgtcact tcttctaatg aagtgaaccg
     4561 cttagtaaca ggacagattc cgcatgactg ataacaatac cgccctaaag aaagctggcc
     4621 tgaaagtaac gcttcctcgt ttaaaaatcc tggaagttct tcaggagccg gacaaccatc
     4681 acgtcagtgc ggaagattta tacaaacgtc tgatcgatat gggtgaagaa attggtctgg
     4741 ctacggtata tcgcgtactg aaccagtttg acgacgctgg tatcgtcacc cgccacaatt
     4801 ttgaaggcgg taaatccgta tttgaactga cacagcaaca tcaccacgat cacctgattt
     4861 gcctcgactg cggcaaggtt atcgaattta gtgatgattc catcgaagcg cgtcagcgtg
     4921 aaattgccgc aaaacatggc attcgcctga ctaaccacag tctctatctt tacggtcact
     4981 gtgccgaagg cgattgccgc gaagatgagc acgcgcacga aggcaaataa gccagcctga
     5041 acgagaaaag ccaacctgcg ggttggcttt tttatacaag ggaaataaga aaagcgagag
     5101 ttacagctct cacttatttg ttattactgc gtatttcctg ccagacctta tcacaatcgg
     5161 ctttcaccgc ctgatcattt ccggtttgcg ttgcacgcag acactgctga taatccagta
     5221 cgcgtacact ttcctggtcg gcaaactgct gatgttgttt ctctttcttc agcacgttta
     5281 acacgctctg gcaggcttca attttttctg gcgaaccttg cgcagtgttg atacaggcgc
     5341 tgtatgcctc tttcagacgg ctgtcttcct gtggtgcggt tgattgcgca caagctacca
     5401 gccccgatgc catcatggcg atgagaatca gttttttcat tgaatgcggc aggcacaaaa
     5461 cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat ttcacgtcac
     5521 gctcatcctg gaagatgtta ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt
     5581 cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt
     5641 agaccgcatc caggctgtag gcagactctt caatagtccg ttttttgtcg tagtacgcat
     5701 ccggattgct ctgccaggtc gcaggtttag catcccatgc gtaaacgtag gaagcgccga
     5761 tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag aagaccgctt
     5821 tttcgccgtt agcgttgaag tcggaacggt tatcccacca gatatccagg cggccgttgg
     5881 aggaagcgta ggttggggtc atacgttgca ggaagtatcc ctgctgactg tcagccttaa
     5941 cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaacg
     6001 cttgcagcta ggcggtgccg tcataaagat cgttgacgct gcgatcgtca actttatcgc
     6061 gggtaccata gaactggtag ctggtggtta acgggctacc ggcgatatca aatttgtagc
     6121 tggctttggc aaaatattga tcgatatacc cttccgcctg accaaatgcc gcttccagta
     6181 cgaagttatt tttgaagtcg tatttcgcac cgatggagtg cagataatca actttggtgg
     6241 ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggcgctttg tattcgttgg
     6301 tccacatgta ggagaaactc aaggcaccag catcgccgta atcaaaattc gccccggctt
     6361 ccgccccctg ataagtaccc ggcataaagc tccagtgcgg cgctaacagc gtttgaccgg
     6421 ttggctgaat gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg gccgcagctt
     6481 tatacaggct tataccgctt ttgtcgccgg accagtcttc gtcataggct ttattacttt
     6541 ttgaaaacgc gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt tcaatcgccg
     6601 taaacgcagc aatatcaagg ccgaacatat cagcagcata accggactga aaatagaggt
     6661 tggcattcca ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt
     6721 tacggtcgcg ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcgatga
     6781 atccttcggc gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcaa
     6841 tagccagcgc cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga
     6901 cgtataaatt gctgcaccaa aggtgaacag cgaaaaaaaa taaaaattaa aaaagtagcg
     6961 ttgagaaaag tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc
     7021 tctttctgta accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt
     7081 acaaggctta agtttgcggt tgaaaggctt gcagaagaat aaaaacgtga a
//
LOCUS       DADLSB010000181         7126 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.181, whole genome shotgun sequence.
ACCESSION   DADLSB010000181 DADLSB010000000
VERSION     DADLSB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.181"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            154..1197
                     /locus_tag="JN331_003584"
     CDS             154..1197
                     /locus_tag="JN331_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY7261617.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            1712..2173
                     /locus_tag="JN331_003585"
     CDS             1712..2173
                     /locus_tag="JN331_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261618.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTDPTHVMWTRP"
     gene            complement(2170..3025)
                     /locus_tag="JN331_003586"
                     /pseudo
     CDS             complement(2170..3025)
                     /locus_tag="JN331_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3022..3321)
                     /locus_tag="JN331_003587"
     CDS             complement(3022..3321)
                     /locus_tag="JN331_003587"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7261619.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK"
     gene            3783..4040
                     /locus_tag="JN331_003588"
     CDS             3783..4040
                     /locus_tag="JN331_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261620.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            complement(4681..4953)
                     /locus_tag="JN331_003589"
     CDS             complement(4681..4953)
                     /locus_tag="JN331_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261621.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            5042..5221
                     /locus_tag="JN331_003590"
     CDS             5042..5221
                     /locus_tag="JN331_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261622.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            5273..5467
                     /locus_tag="JN331_003591"
     CDS             5273..5467
                     /locus_tag="JN331_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261623.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(6266..6602)
                     /locus_tag="JN331_003592"
                     /pseudo
     CDS             complement(6266..6602)
                     /locus_tag="JN331_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2069 a         1601 c         1508 g         1948 t
ORIGIN      
        1 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
       61 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
      121 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
      181 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
      241 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
      301 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
      361 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
      421 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
      481 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
      541 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
      601 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
      661 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
      721 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
      781 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
      841 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
      901 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
      961 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
     1021 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
     1081 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
     1141 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
     1201 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
     1261 cttccccaag tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
     1321 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
     1381 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
     1441 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
     1501 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
     1561 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
     1621 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
     1681 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
     1741 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
     1801 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
     1861 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
     1921 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
     1981 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
     2041 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
     2101 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
     2161 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
     2221 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
     2281 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
     2341 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
     2401 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
     2461 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
     2521 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
     2581 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
     2641 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
     2701 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
     2761 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
     2821 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
     2881 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
     2941 agccgctgcg ggcaacccga agtacacgac acatcgcttt aatgctgaac tctgcccgat
     3001 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
     3061 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
     3121 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
     3181 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
     3241 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
     3301 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
     3361 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
     3421 atcatctgta agcgcagtaa taccattagt agcacctctc ggcggcgcat tgattattga
     3481 atattataac tggaatgtta tatttcttgt tatgatgctg ttcagtatta taataatgtt
     3541 attatcacca aagataactc atgcaaatga gaaagtaata tgtcaaaaca gtgcatcctt
     3601 tgtgaccaaa gagtttatct gcggcactat acttgtctca ttaatactca gttctctatt
     3661 ttgctacata tcaatatcac catcaatttt catgcatact tatggcctga gtacttttag
     3721 ttactcaatt attttctcct tctcagtttt atttttcata atcggaaatc aattatcaaa
     3781 gattgaaaaa ttatccaacc tatggttttt attacccaat agccaatttt atcgcattac
     3841 aaataaatgg ttcaaaaaca ggatttgccg catctattac aggttttact caaacaataa
     3901 gcgctggtac aatcagtttc ctgtcagtaa ccgtaagcga caacggtgtt ggtcccggga
     3961 ctactttagg tcttggttca ctaataactt cattactgtc aatagccgta atcgccattg
     4021 caggaaataa taaaaagtaa gtgtactctg actgcgtatg tatctacttt gtgtaatgca
     4081 ggtgtctatt gaaaataatt cttttcgtgg gcatctgttg tataaaataa aaaattatcg
     4141 taagtactcc atctgcgatg tcttgtgaaa attatcctgt ctggcaacaa tcgcgtccat
     4201 ctgtattaat ggactcgaac gatgaactct caaacaacac tagatatcac cggctttcgc
     4261 acttatttgc cagaatcatt acgtctcaga ttcgacgagc aattatctgt ccgggctatc
     4321 gcacagcagt taagactcag ttattccccc attcacaatc tctttcaacg aattaacgcc
     4381 tcaggtattg tctgaccatg acccgagtcc ctttcattcg cccaaccgat accatacttt
     4441 atgccaacag aaagaatgaa tgaaccgcgc ctaaaatcag cgaggacaca tggcacaaag
     4501 tgcgacatgt ctcacattta accccagtgg gggcaacaag tataaaaccg ttttttgatt
     4561 aagtggtcgg atatgaaaag gggaaggttt atgtgaaaaa tacctgtgtt ttctttgaaa
     4621 acacacaaca gtatcaggtt atttatgttt atacagtgcg ttccaaactg acctgaataa
     4681 ttacgcaagt tgcaacaata aaacattttt gccgtagtgt gaggtactcc ggtaaattgc
     4741 tttccaggtg cagacaatga ctaacgaaga actacaggca atcgcattat ccatgcttga
     4801 gcgccagcgc gaaaaagtac accagcaacg agagtctttg cttaacccat ctccattcac
     4861 caggccaaaa cgattggagc ttgaagcatt cttggacaaa tacccacggc ggcttaaatc
     4921 aggcaaaacc agaccaccag gctgatagct cacagtttgt tctccgattc aataaaacgg
     4981 ataagaacca tcagcgcggt agcgtaaggc actacagcgc attgctttac gagtaataac
     5041 aatggattta tcaccagaag acgcaaagtt tttacgagaa ctgcaacacc gtatgctcag
     5101 agaagccatt atcgaacagc aaacgggcat gagcgccata gacccattgc gatacaaacg
     5161 ggcctcatct cctttagttg acgacaattc caatgcggat aaaacacgtc caccacactg
     5221 attacatttt tcattagcac agtggtgtga tgcagcatac gaggaaaacc ggatgaatga
     5281 gaacaaaata aaaaggctgg agcaactgtt acaggcacgc caacgggagt ttgccactaa
     5341 atggggacgt gccgcgctgc cacatgaacg cctaacctat tttccacttg aagacctcat
     5401 taaaaataat ggcaaaaaaa tcaggtctga taagaaacac ttcttagaat gcaatgaaga
     5461 caaataacaa caatctgcat attgccacca accacagatg tggcatcgtt atcagcgcac
     5521 ttgattttct ttatatccac tgacgagctt gccggggcac ctttgtttgc cggtcattct
     5581 ggtatcatgc agatatttac ggcgtacaag cccactagca tgacattgta ataaggcctg
     5641 agcctaacgc tttaatgtgt gtcatacact cagtggcgta atcgttgaca atatggtgca
     5701 tgtaatcgta agcgtccagc gaaatccatc tggtcataat ctgaagcatc cgacaaagtg
     5761 gtgtccacca aataagtgat ctgcgcccaa tatcctgagc cggaactttg acaaaggcca
     5821 acgaaaaatg ggagcggatg ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg
     5881 ccagttctcc gatctgttta accgggaaat tatctcctac agcttgtcag aaaggtcggt
     5941 gatggagcat cgttaatacg atgctatacg atgcattcac agtgcccggc cagaggatgc
     6001 cccgtcgctg catatggatc agggttggca atatcgaatc gcaggctata ggcaaagtta
     6061 aagccccatg gagtagcaca aaatatgccg cacaaaggaa acggtctgaa taacgcagtg
     6121 atgaagaact tcttcagcac actgctaaaa cgcatagtga tcgagcgctg attctggcga
     6181 acaactgaac taatacatca gaatctgcat tatgttaaat aaatatgaaa agatggttta
     6241 aataccccgt tacttttgac ttacactata cggtatcgca tcgtttaata ttcgcaccgg
     6301 ccagattttt atttctatta gttgtcacaa tactgaatgc gtacgaccac agtattctgg
     6361 ctcctgtgtg gttatgcttt aattctgcgt tccgggcaga taagcagttg cttgcaggaa
     6421 tccttcttgt gttaatgtca gttccccttt ttaccagtgc tgatttccac attccgtcca
     6481 acagagctta tagcctttcc ctggattata gcattgtccg gctgaagttc tttttgaata
     6541 ataatagaag cactgctggc agatccagtc cgtttttcat aacccactgt actgataacc
     6601 ataatctaat cagtagaaat tgagtcaaaa ataagcacta ctccatacag gataattaga
     6661 gatcagtttg attattcaca attcatcatc agcattttct atttctgacg aaatcaacat
     6721 gaaaataacc atatatgata attattataa taacggtttt aattggaata catatattac
     6781 aacgtattat atataattgg tattctggga actatattct caaaatacag tagaaacgag
     6841 gtatgtttct ggtggaaagg acagttggat taaaaagtaa gaattgataa aaaaaaacgc
     6901 cagcaacaca tagtgccggc ggagggaata ccccatggag aaaatgtgat gcctagagca
     6961 tcatgaatat ataattaaaa atagttagcg ttgtcaatac tttcacaaaa ataattttcg
     7021 tagtatagaa agatattttt atgcatgacc tacctgaatt tgctccgggt agaggttata
     7081 aataaaaatt gaatcacgac aaacacaata ttcatagtat ggcgat
//
LOCUS       DADLSB010000182         7124 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.182, whole genome shotgun sequence.
ACCESSION   DADLSB010000182 DADLSB010000000
VERSION     DADLSB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.182"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..273
                     /locus_tag="JN331_003593"
     CDS             <1..273
                     /locus_tag="JN331_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="allows for ions and hydrophilic solutes to cross
                     the outer membrane; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY7261624.1"
                     /translation="QFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVG
                     ATYYFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            414..848
                     /gene="uspF"
                     /locus_tag="JN331_003594"
     CDS             414..848
                     /gene="uspF"
                     /locus_tag="JN331_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY7261625.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1024..1959
                     /gene="ttcA"
                     /locus_tag="JN331_003595"
     CDS             1024..1959
                     /gene="ttcA"
                     /locus_tag="JN331_003595"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY7261626.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2088..3461)
                     /gene="dbpA"
                     /locus_tag="JN331_003596"
     CDS             complement(2088..3461)
                     /gene="dbpA"
                     /locus_tag="JN331_003596"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY7261627.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3939..4922)
                     /gene="zntB"
                     /locus_tag="JN331_003597"
     CDS             complement(3939..4922)
                     /gene="zntB"
                     /locus_tag="JN331_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY7261628.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGSLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5177..6409
                     /gene="dgcM"
                     /locus_tag="JN331_003598"
     CDS             5177..6409
                     /gene="dgcM"
                     /locus_tag="JN331_003598"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HAY7261629.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLTA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHNPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARPLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6430..6993)
                     /gene="smrA"
                     /locus_tag="JN331_003599"
     CDS             complement(6430..6993)
                     /gene="smrA"
                     /locus_tag="JN331_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY7261630.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKAAQAKQENWERHAKRSR"
BASE COUNT         1850 a         1825 c         1678 g         1771 t
ORIGIN      
        1 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
       61 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
      121 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
      181 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
      241 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
      301 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
      361 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
      421 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
      481 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
      541 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
      601 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
      661 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
      721 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
      781 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
      841 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
      901 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
      961 gctgggcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     1021 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     1081 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     1141 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     1201 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     1261 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     1321 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     1381 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     1441 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     1501 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     1561 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     1621 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     1681 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     1741 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     1801 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     1861 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     1921 ttggatgaat tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     1981 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     2041 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     2101 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     2161 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     2221 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     2281 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     2341 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     2401 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     2461 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     2521 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     2581 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgatcgcg ttgctccaga
     2641 tcgccgtgta acgacaatgc actttgctct acttcattca gcgcgtcgca gacagcctgg
     2701 caatcttttt tggtattgca aaacaccaca caagaggatg gctgatgcaa gcttaataac
     2761 cgttgcaaca gaggaatttt gcctttactg gatgtctcat aaaattgttg ttcaatgggt
     2821 ggcaaagcat ctgttgagtc aatttcaatc gccaaaggat cgcgttgcac tcgtccgctg
     2881 attgcggcga tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg agatgcaggc
     2941 gcaaaacgga tgacatcatc aatggcatcg ctaaatccca tatccagcat gcggtcggcc
     3001 tcatccatca ccaaagtatt cagcgcatcc agtgataccg tgcctttttg caagtgatcc
     3061 agcaaacgac ccggcgttgc cacgataata tgcggcgcat gttgcaacga atcacgctgc
     3121 ataccgaacg gttgaccacc gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc
     3181 agccgacgca attcacctgc cacctgatcc gccagttcac gcgtaggaca cagcactaaa
     3241 gcctgggttt gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc
     3301 gttttgccgc tgccggtttt cgcctgcacg cgaacatctt ttccggcaag gatcgccgga
     3361 agcgcggcgg cctgcaccgg cgtcatggtt aaataaccca actcattaag gttcgtgagt
     3421 tgggcgggag gcaaaacatt cagggtagaa aaagcggtca caatctattc tcgtggtcat
     3481 cgacgcaaag ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc gacaatttaa
     3541 tcggttcttc atcgggtggt gggtcaggca tgggttgcgg gcgagggatc ggatcgggca
     3601 ctggaacagg atcgccagga atcggttcag ggacaggaat ttgcaaataa ataagtgtcg
     3661 tcatatttcc ctccggtcat tgggtggact cttaaagggt agacgctgat aaataacagg
     3721 caaaaaaaag ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat
     3781 tcaaaaaagg aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc
     3841 tgcaagggag atattgccct gaatgggtag agagtttatt gacttcgctc aaactttgcg
     3901 gcgtttttgt atacagacag ccggaaaaat tgcttttgtt acaaccattt actacggtgc
     3961 aaccataaag caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg
     4021 aattgccacc cgccgccagg gatcccacca aggttgacgc caaataaccc ggtcagaaag
     4081 gtactgggta aaaagaccat tgccatcaac gacattgtat aggtacgacg agctaaattt
     4141 tcctgcatca cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg
     4201 atttcgtcaa ggccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca
     4261 tcgctcatcc acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc
     4321 atatagcgac gcatcacaat taattgtttg cgcagcagag ccaggaatcc acgcggtgga
     4381 atttgctgat caaggagatt atcttcaagg tcgataattt tatcgtgcag ctgctcgata
     4441 aattcactgg aatgatcggt caacgcatcg cacacatcca ccagccatcc cccgcaatcg
     4501 gtcggacccg tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt
     4561 tgtcgggtcg aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg
     4621 gggcgttcat cggtgctgcc gtttatacag cgcaatgtaa tcagcgagcc ttcaccgaaa
     4681 cggctgactc ggggccgtgt gctctcgccc gccagcgcat cacgtacgtt attgggaagc
     4741 agcggtgttg tcgccagcca ttgggcgcta tcatggtgta cataattaag gtggagccag
     4801 cagggatgcg cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca
     4861 tccagcatcc aggcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc
     4921 acagtgcctc catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa
     4981 catctcatca ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa
     5041 aagcaaaatg cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc
     5101 agaattatct gatcatatga cgtggctttt ttgcgatcgg atagcaacaa aaattgataa
     5161 aaataacggg atctcaatga ttacgcacaa cttcaatacc ctggacttac tcaccagtcc
     5221 tgtctggatt gtttcgccct ttgaggaaca gttaatttat gccaatagcg cggcgcgact
     5281 gttgatgcaa gacctcacgt ttagtcagct acgaaccgga ccctattccg tctcctcaca
     5341 aaaagaactg ccgaaatacc tctccgatct gcaaaaccaa cacgatatta tcgaaatcct
     5401 cactgttcag cgtaaagaag aggaaacagc attaagctgt cggcttgttt tgcgagagct
     5461 gacagaaaca gaaccggtga ttattttcga aggtatcgaa gcgccggcaa cgctgggttt
     5521 aaaagccagt cgctcggcaa attatcagcg caaaaaacaa ggtttttatg cgcgcttttt
     5581 tctgactaac tctgcaccaa tgttgttgat tgacccgtca cgagatggac aaatcgtcga
     5641 tgctaacctc accgcgctca atttctatgg ttataaccat gaaacgatgt gccagaaaca
     5701 tacctgggaa ataaatatgc ttgggcgtcg cgtcatgcct atcatgcatg aaatttcgca
     5761 tttacccggc ggtcataacc ctttgaattt tgttcataaa ctggcggatg gatctactcg
     5821 tcatgtgcag acctatgccg gaccgattga aatttatggc gacaagctca tgttatgtat
     5881 tgtgcatgat attactgagc aaaaacggct ggaggagcag ctggaacatg ctgctcacca
     5941 tgacgcgatg accggattac tgaatcggcg acagttttat cacattacgg aaccaggcca
     6001 aatgcagcat ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt
     6061 taaacacatt aacgatctct atgggcattc taaaggtgat gaggtgttat gcgccctcgc
     6121 ccgccccctc gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga
     6181 gtttgtctta ttgctaccaa gaaccccact ggataccgcg ctttcgctgg ctgaaactat
     6241 ccgcgtaagc gtggcaaaag tgagtatttc gggcttacca cgctttaccg tcagcattgg
     6301 tgtggcgcat cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc
     6361 tttgtatcgg gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag ccgcggatgc
     6421 gtctcgagat caacgactgc gcttagcgtg gcgctcccag ttttcttgct tcgcctgcgc
     6481 cgctttacgt agtgcgacgt aacacgcccc gctgccgcca tgatgcggta gcgcggtgca
     6541 atatgcctga acatcatcaa attcggtcag ccagcgcgcc acatagctgc ggacaatatt
     6601 ggcatgtgat ttatcttccc gccctttacc atgaataatc agcacattac gcaaaccatc
     6661 cgccagggct tgttgaatga aactgaacac cattttgcgg cactcttcca ccggctggcg
     6721 caaaagattc aggctcgcct gttgcggata tttaccactg cgcagcttat ccagcacccc
     6781 atgctgcaac ccttcctgcc gaaactccag cggctgactt agcgggatga tgtcgagaaa
     6841 tccggtagtg aggaaattat caagctgcag cgtgtcgata cgctgcggcg cacgttggtt
     6901 acgcgttgga tgccagtgga catctgtagc acgtttcagc ggctggacat cttccatggc
     6961 gtcaagaaac agcgatttgt cgtcaaggtt catgtaacct cctatgcacc ttcacacgtc
     7021 gtgattcaga cgggttaatt gttcatactt actgcatcgt tcccttcaca gattgaaaca
     7081 atactctact tcatacctgt gagtatctgc gcaggcgggg catt
//
LOCUS       DADLSB010000183         7072 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.183, whole genome shotgun sequence.
ACCESSION   DADLSB010000183 DADLSB010000000
VERSION     DADLSB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.183"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..102)
                     /locus_tag="JN331_003600"
     CDS             complement(<1..102)
                     /locus_tag="JN331_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006660997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261631.1"
                     /translation="MTILNKALTRPAALWGVPLVPLLFTLCILTLAAV"
     gene            complement(112..411)
                     /locus_tag="JN331_003601"
     CDS             complement(112..411)
                     /locus_tag="JN331_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HAY7261632.1"
                     /translation="MTLRTIKTAATRLIAAFSALSAVPAMAASADQAFQKGSDAINWV
                     FLGLTGMSVVTISCAVAFIAYKMLVDGKAWHDLQRVFWAGVLLAGASGFGAYFAA"
     gene            complement(422..1084)
                     /locus_tag="JN331_003602"
     CDS             complement(422..1084)
                     /locus_tag="JN331_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY7261633.1"
                     /translation="MLTTAALFQLAMQCAPAVHPDTIHDITRTESGLNPYAIAEIVPV
                     KGGRSRVISHLPSSKDEALKIVEAIKQKKHRYSVGLMQITSTNFPQFGVSAESMFNPC
                     DNMTVAAKIITDCYQRGGTLQRALSCYYSGNFETGQRPESAFGNTSYVQRIGYVVPST
                     RAEQQTASPSSGEAPAVPSDNTVYPDSVIRGVIPAPDTTPTSIAAYPPHVVRGGLAVS
                     SD"
     gene            complement(1087..1302)
                     /locus_tag="JN331_003603"
     CDS             complement(1087..1302)
                     /locus_tag="JN331_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261634.1"
                     /translation="MPDITPAPASLLEAVFTPMTEYTHNVYSVKGDRLAMTLTKIGVA
                     QANSQGDIQLFINPDCATGKVLLYRKD"
     gene            complement(1295..1603)
                     /locus_tag="JN331_003604"
     CDS             complement(1295..1603)
                     /locus_tag="JN331_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261635.1"
                     /translation="MSMQTVRRKALRQNPYSTLGKPPLRSSRDRITRAINGTWSLREL
                     PDNVRELLIERAALRAEITTLTVKAQGLKKSDVSVLIADRLRRIDAITSLLENRKCNA
                     "
     gene            1917..2831
                     /locus_tag="JN331_003605"
     CDS             1917..2831
                     /locus_tag="JN331_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY7261636.1"
                     /translation="MLEMFEKIEKKYWQDKIKKISKFRVIKMQDLDDAKVIEVLAKVL
                     TKKGISKVKHNSYISKVLQITTSAGYKKLNGATKWEVQQLIKVVQSVGMDMIEFFRIY
                     YEDNKEIQDAIWNDGRSEHSCKVCLYPEGSEVNTEYSALKVADQWNIFPTNDLKDELL
                     YESKRSIESIVINPKVISSTKYRIALLDDDANITESLKEILSNDYYQVDTYDNLKKLT
                     QAVATNPYDAYVLDWIIGDETSFKLVKAIRSSKNKNAMIIVLTGQLSGIKDDEIVKSI
                     HDYDIVGPYEKPIKSGIIKSNIEKYFAR"
     gene            2835..3818
                     /locus_tag="JN331_003606"
     CDS             2835..3818
                     /locus_tag="JN331_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HAY7261637.1"
                     /translation="MRNLLEYSGSLISNDSLIVAPYDCQKLSYENILPLNNGLIKNWN
                     IQKFSSDYSEIEHLNVINGMGVTLGDSIVGISVLSAIKKKNPKIKIKLIRPETAPSYV
                     EELYELASSVIDELHYMPFHIQKLPCTALNIDVGNQLYSPDFHIMEMHDYFIQGLGVK
                     LDDVNHNELSNKWLGNIDLGAPVFENYTLFAPIASTKIRSIPSKFYYDIVDMLSRREG
                     KTVLGFVDVNHKNYINISKYSPHTKDFIALIKYASNVYTCDSSALHIAAGFGVPTECV
                     FNTIPPELRTKYYSKCKSIYVGNKRLEKIQSSEDFQLVKMVENNYREYLYG"
     gene            3811..4299
                     /locus_tag="JN331_003607"
     CDS             3811..4299
                     /locus_tag="JN331_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7261638.1"
                     /translation="MVKIISFLSLMLLSISAYSNIGDIVIDGSIANANSDKGYVITEK
                     QFDSFPRDTIVTTTPWTPTGKKVKFKGVDLSYVLKLAGANGAKLKFHALNDYEIIVDM
                     NDVEKYNILLATEMDGKRLQIRDFGPYFLVYPLDEHYIELNTPHYLARFIWQIDKITV
                     LK"
     gene            4296..6014
                     /locus_tag="JN331_003608"
     CDS             4296..6014
                     /locus_tag="JN331_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HAY7261639.1"
                     /translation="MKKNFFIILVALLLLIAFTVSSVVKYTSLLSEKNLYTVAGTQEN
                     YGFSVAKFIIQLNELYTLINSDNTIDDVRLKFDILYSRLNVIYVKSEATAPLYKQQGY
                     EETIDSINKKLEDIDGLLSHNHPDYKKISTIIADIKPLTKTVTNLADMAEIAQRNDAL
                     KDFRSKRQQLWTLLFITGGLISLLLFVLFIYISKINRLLLSERAAFASKNAFLGMVGH
                     ELRTSLQAIVSIIDVVTNNLSGGIKSGQIERLETAVSKMERQLNDLAEFAKIDNGSVE
                     IKNTYNSLQAIVTNAVQDCIAIYEKKDVTVKIKNNNDAVIFTDALRLNQVIENLTSNA
                     IKYTERGEVNVDYFIEKGKVLNIVISDTGKGIPKDKLKFIFKPFTRVVDSKSTVPGFG
                     MGLAIVAGIIRLLKGTIHISSEVNVGTTVTVRIPIKLGDSSLATAEVSASAPGDVFGV
                     LSLLVIDDNEMACSSLSSLLTNAGYIVEATTSPERALEKLLRKPYDVVLSDLQMPGMT
                     GDELYATVSSSQNPNRSTPFIFISAYSDAHSIGQAPLLTKPVRLKDINLKIHEVMSNQ
                     GRVKSE"
     gene            6278..6487
                     /locus_tag="JN331_003609"
     CDS             6278..6487
                     /locus_tag="JN331_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261640.1"
                     /translation="MLLTLRGMFFSYFSDTRELNTVVAKLYPLKMSQMPLYAVGVVPA
                     RRQPQPGRISALAGRRAAYERVPDN"
     gene            6696..6830
                     /locus_tag="JN331_003610"
     CDS             6696..6830
                     /locus_tag="JN331_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261641.1"
                     /translation="MGALSDVAEPARPSGRCKSGHSPEPVHRVGDEEVMCLQDLLKDL
                     "
     gene            complement(6873..7072)
                     /locus_tag="JN331_003611"
                     /pseudo
     CDS             complement(6873..7072)
                     /locus_tag="JN331_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2066 a         1424 c         1682 g         1900 t
ORIGIN      
        1 gacggccgcc agggtaagga tgcacagggt gaacagcagg gggacaagcg ggacccccca
       61 cagcgccgcc ggtcgggtca gcgccttgtt gaggatggtc atgctgcctc cttaggcggc
      121 gaagtaggca ccgaacccgg acgccccggc gagcaggaca cccgcccaga acacgcgctg
      181 gagatcgtgc caggccttgc cgtcaacaag catcttgtag gcgataaagg ccaccgcaca
      241 gctgatagtg acgactgaca taccagttag gccgaggaag acccagttaa tggcgtcact
      301 gcctttctga aatgcctggt cagcgctggc cgccattgct ggaacagcag acagggcgga
      361 aaatgcggca atcaggcggg ttgcagcggt tttgatagta cgtaaagtca tgatctcttc
      421 cttaatcaga agacacagcg aggccgccgc gcacgacatg tggcggataa gcagcgatgc
      481 tggtcggggt ggtgtcgggt gccggaatta cgccgcgtat gacgctatcc gggtaaacag
      541 tgttgtcgga cgggacggca ggcgcttccc cggatgaggg agatgctgtc tgctgttctg
      601 cccgcgttga gggcacaacg tagccaatac gctgtacgta gcttgtgttg ccgaacgcgc
      661 tttccgggcg ctggccggtt tcaaagttgc ctgagtaata gcagctcagg gcgcgctgta
      721 atgttcctcc tcgttgatag cagtcggtga tgattttcgc ggcaacagtc atgttgtcac
      781 aggggttaaa catggattcg gcgcttacgc cgaactgcgg gaagttggtg ctggttatct
      841 gcatcagccc tactgaataa cggtgctttt tctgctttat ggcctccaca atttttagcg
      901 cctcgtcttt cgacgaaggc agatgagaaa tcacgcggct cctgccgccc tttaccggga
      961 caatctcagc gatggcatat ggatttaagc cggattcggt gcgggtgatg tcgtggatgg
     1021 tatcggggtg aacggcggga gcacactgca ttgcgagctg aaacaacgcg gcagtggtta
     1081 acatagtcag tcctttctgt agagcagtac cttaccggtg gcacagtccg ggttgataaa
     1141 taactgaatg tcgccctggc tgttagcttg ggcaacaccg atttttgtaa gcgtcattgc
     1201 cagccgatca cccttcaccg aatacacgtt gtgcgtgtac tcggtcatgg gggtgaatac
     1261 ggcttcaagc agtgaggccg gagcaggcgt gatgtcaggc attacatttc ctattttcca
     1321 gcagtgaggt gattgcatcg atacgtcgca gcctgtcggc aataagtacg gatacatcgg
     1381 attttttgag tccctgcgct ttcacagtca gggtagtgat ttctgcccgc agggctgccc
     1441 gttcaattag caattctcgg acgttatccg gcagctccct gagcgaccag gtgccattaa
     1501 tggcacgcgt gatacggtca cgggaggagc gtagcggcgg cttgccgaga gttgaataag
     1561 gattctgacg cagcgcctta cggcggacgg tttgcatgct catggctgtt tctcctgggt
     1621 atgagtaagc cacccgcgtg cggtgcacag tgggagacag ggaaggatta aacgggaggc
     1681 ttaacgatac agaggagtga gagtgttacc cattacaggc gcggcacgtc atctctttac
     1741 gcagtcacag aaaacacctc cttatcctga ccggggaacg ccgctgctag gttattaaag
     1801 agctaatgat ttgattaata caatagtgct tataagttgg gtattcaata aaattataac
     1861 ttatagtgtc gatttaagaa tgattccaat ttttaggact ttttcagctg tgtaaaatgt
     1921 tggagatgtt tgaaaaaatt gaaaaaaaat actggcagga taaaatcaag aaaatctcca
     1981 aattcagagt aataaaaatg caagatctcg atgatgcaaa agtgatagag gtactggcga
     2041 aggtattgac caaaaaaggt atttccaagg taaagcacaa tagttacata agtaaggtgc
     2101 tacagatcac tacatcagcg ggctataaga agttgaatgg tgcgaccaag tgggaggttc
     2161 agcaactcat aaaagtagtc caatccgttg gcatggatat gattgagttt ttccgtattt
     2221 attatgagga taataaagaa attcaagatg cgatctggaa cgatggccgc agtgaacact
     2281 cttgtaaggt atgcctctat cctgaaggtt cggaagtaaa cacagaatac tcagcactga
     2341 aagtagctga ccaatggaac atttttccga caaatgattt aaaagatgaa ctgctttatg
     2401 aatcaaaacg aagcatagaa agtattgtca taaatccaaa ggtaattagc agcactaagt
     2461 atcgaattgc actacttgat gacgatgcta atatcaccga aagcttaaaa gaaatccttt
     2521 cgaacgatta ttatcaagta gatacttatg ataacttgaa aaaacttact caagctgtcg
     2581 caacaaatcc gtatgacgca tatgttttgg actggatcat cggtgatgaa acaagcttta
     2641 agttggttaa agctataagg tcgtcaaaaa ataaaaatgc aatgataatc gttcttacag
     2701 gtcaactatc tggtataaaa gatgatgaaa ttgtaaagtc aattcatgat tatgacattg
     2761 tgggaccata cgaaaagccg attaaatcag gcatcatcaa atctaacatt gaaaaatatt
     2821 tcgccagata gattatgaga aatttacttg aatattccgg tagcttgata agtaacgata
     2881 gtttgatcgt agcaccttac gattgtcaga agctaagcta tgaaaatatt ttaccattaa
     2941 acaatggtct tataaaaaac tggaatatac aaaaattctc aagtgattac tctgaaatag
     3001 aacacctaaa tgtgatcaat ggcatgggag tgacattagg tgattctatc gtcgggattt
     3061 ctgtgctgtc agcaatcaaa aaaaagaatc ccaagattaa aattaagctt attcgaccgg
     3121 agactgcacc gtcatatgtt gaggaactgt atgaattagc atcctcggtt atagatgagc
     3181 ttcattacat gccttttcat attcagaaat tgccttgtac tgcattgaat attgatgtgg
     3241 gaaatcagct atattctcca gatttccata ttatggaaat gcatgattat tttatccaag
     3301 gtcttggcgt gaaacttgat gatgttaacc ataatgagct atctaataaa tggttaggca
     3361 atattgatct cggagcgcca gtttttgaaa actacacact ttttgcacct attgctagca
     3421 caaaaataag aagtataccg tcgaagttct attatgacat agttgatatg ctttctaggc
     3481 gcgaaggaaa aacggtacta ggtttcgtgg atgtgaatca taaaaattat attaacatat
     3541 caaagtattc accccatacg aaagatttca tagcgctaat taagtatgct agcaacgttt
     3601 atacttgtga ctcctctgca ttgcatatag ccgctggatt tggtgtacct actgaatgcg
     3661 tgtttaatac aataccgcca gaattaagaa caaagtatta cagtaagtgt aagtcgattt
     3721 atgttgggaa taaaagatta gaaaaaattc agagtagtga agatttccaa ctggttaaaa
     3781 tggtggaaaa taattacagg gagtatcttt atggttaaaa taatatcatt cttatcccta
     3841 atgcttttgt caattagtgc atatagcaat attggggata tagttataga tggttcaatc
     3901 gctaatgcta acagtgataa aggttatgtg attactgaaa agcagtttga ttcgttccca
     3961 cgggatacca tcgtaacgac aacaccttgg acgccaaccg gtaaaaaagt aaaattcaag
     4021 ggcgtcgacc taagttatgt tttaaaactc gctggcgcta atggagcaaa gctaaaattc
     4081 catgctctga atgattatga aatcattgta gacatgaatg acgttgaaaa atataacatt
     4141 ttattggcta cagaaatgga tggaaaaagg cttcaaataa gggattttgg accttatttt
     4201 ttggtatatc ctttagatga acattatatt gagcttaaca cgccacacta tcttgcacgg
     4261 ttcatttggc agatagacaa aattacggtg ctcaaatgaa aaaaaatttc tttatcattt
     4321 tggttgctct gcttttgttg atagcattca ctgtgtctag tgttgttaag tacaccagtc
     4381 ttttatcgga gaaaaattta tacaccgtcg ctggcaccca agaaaactac ggtttctcag
     4441 tggcaaaatt cattatacag ttgaatgagc tttacacgct aatcaatagc gataatacta
     4501 ttgacgatgt taggcttaaa ttcgatatcc tttattctcg tttgaatgtt atttatgtaa
     4561 agagtgaggc gactgcacct ctatataaac agcaaggcta tgaagaaaca attgattcga
     4621 taaacaaaaa gcttgaagac atagacgggc tgctttcgca taatcaccct gattataaaa
     4681 aaataagtac aatcatagct gatataaaac cgctaactaa aaccgttaca aatcttgctg
     4741 atatggctga aatagcacaa aggaatgacg ctcttaaaga tttcaggagc aaacgtcagc
     4801 agttatggac tctgcttttt attacaggtg ggttgatttc cttacttctt tttgtcctat
     4861 ttatttacat aagtaaaatt aaccgattgt tactatcaga acgggctgct tttgccagta
     4921 aaaatgcatt tttaggtatg gtaggccatg aactcagaac atctttacag gcaatagtgt
     4981 ctatcattga tgtcgtaacc aataatctga gtggagggat taaatctggt cagatagaac
     5041 gacttgaaac tgctgtttcg aaaatggagc gccagttaaa tgatttggct gaatttgcaa
     5101 aaattgacaa cggatctgta gagataaaaa acacctataa tagcttgcaa gcaattgtta
     5161 ctaacgctgt gcaggattgc attgctattt atgaaaaaaa ggatgtcaca gttaaaataa
     5221 aaaacaataa tgatgcagtg atttttacag atgctctacg ccttaatcaa gtaattgaaa
     5281 acttaaccag taacgctatt aagtacactg agcgcgggga agttaatgta gattacttta
     5341 tagaaaaagg taaggtatta aatattgtca tttctgatac cggaaaggga atccctaaag
     5401 acaaactaaa gttcatattt aaaccattta cacgagttgt agattcgaaa tccactgtac
     5461 ctggatttgg gatgggactt gctattgttg cagggataat cagactatta aaaggtacta
     5521 ttcatatatc tagtgaagta aatgtaggta caactgttac agtgagaata cctatcaagt
     5581 tgggagatag cagccttgca acagctgagg tatctgcttc agctcccgga gatgtcttcg
     5641 gcgttcttag cttgcttgtt attgatgata atgaaatggc ctgttcgtct ctttccagtc
     5701 tgcttactaa tgctggatac attgtcgagg ctacaacctc gcctgaacga gccctggaaa
     5761 agcttctgcg taaaccttat gacgttgtcc tgagtgattt acaaatgcca ggcatgacag
     5821 gtgatgagct gtatgcaact gttagtagca gtcagaatcc taatcgatcc acgcctttca
     5881 tattcatcag cgcatacagt gatgcacact ctatcggaca ggctcctttg ttaactaaac
     5941 ctgtcaggtt aaaagatata aaccttaaaa tacatgaggt tatgtcaaat caaggacgag
     6001 tcaaaagtga gtaggcattg tcgtaatatt tgcgattata gctttggtgt tctcgatcgt
     6061 tgcgaatttt gaaaatttgt ggagtcaaat ggcgccgaaa gcccggcgta gccggttacc
     6121 tggctataaa ctctgccacc ctaccccctg ccctgattag ccttaacccg cttacagcaa
     6181 tgaagaagta aaagtaatcc cttttaaccc cggtgagcgg gctttacggc gtttacgacg
     6241 taaagggcgt gatttccggg gccttagcgg cggcagcttg ctgctgacgc ttagggggat
     6301 gttcttcagt tatttcagcg acaccaggga acttaacacg gtagttgcaa agctataccc
     6361 gctaaaaatg tctcagatgc ctctgtacgc ggtcggagtg gtcccggcaa ggcgtcagcc
     6421 gcagccgggg cgtatctccg cgttagcggg ccgaagggcc gcttacgagc gtgtacccga
     6481 caactgaccg cagctccacc cacagcgatg aagaagagta gttacaacac ccatcattaa
     6541 aagcgacact actcccggcc ttcggccggg ggcggcggcg cttttcagtg gtggggaggg
     6601 gcacccggcc ggcaaaccgg cgccggtttt atgatgagct ttcttttgaa tgctgtaact
     6661 aatttggttc tccggtcggg gcccttcgcg aagcgatggg agctctttct gatgttgccg
     6721 aaccggcgcg accgtcaggt cgctgcaaga gcgggcattc tccggagcct gtccacaggg
     6781 taggcgatga agaggttatg tgtttacagg atttattaaa ggatctttaa aagagataca
     6841 gaactggtgt ttaaaaggaa ctacgcgccc tgaaagaccg acggcaggct cagaagatgc
     6901 tccagcgtgg ccatagtacg ttcaccgaag acgtgtgcaa caaccgtcct ccgtatcctg
     6961 tcatacgcgt aaaacaacca gcgctggcgt gatttagcgc cgacgtaacc ccactgttcg
     7021 tccatttccg cgcaaacaat gacgtcactg cccggttgta tgcgtgagtt ta
//
LOCUS       DADLSB010000184         7005 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.184, whole genome shotgun sequence.
ACCESSION   DADLSB010000184 DADLSB010000000
VERSION     DADLSB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.184"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            242..1219
                     /locus_tag="JN331_003612"
     CDS             242..1219
                     /locus_tag="JN331_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7261642.1"
                     /translation="MILIPDDEPELSLPAASEEFLPALSGENAPVSPARAYLLSLNSH
                     RSRQTMASFLNIVAVMLGAASLESCSWGSLRRHHVMAVTELLRDTGRATATVNTYLSA
                     LKGVAKEAWMLRLMDVESFQHIRAVRNLRGSRLPSGRALPQGEIRALFAVCEADRSCL
                     GARDAAMLAVILGCGLRRSEVVSLDLRDVVTQDRALRVLGKGNKERLAYVPAGAWQRL
                     QIWIDEIRGETPGPLFTRIRRFGDVTLNRLTDQAVYHILQVRQGQAGITKCSPHDLRR
                     TFATAMLDNGEDLITVKDAMGHASVTTTQQYDRRGEQRLQDARDRLNLI"
     gene            complement(1267..1530)
                     /locus_tag="JN331_003613"
                     /pseudo
     CDS             complement(1267..1530)
                     /locus_tag="JN331_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
     gene            complement(1565..3316)
                     /locus_tag="JN331_003614"
                     /pseudo
     CDS             complement(1565..3316)
                     /locus_tag="JN331_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
     gene            3320..3712
                     /locus_tag="JN331_003615"
     CDS             3320..3712
                     /locus_tag="JN331_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY7261643.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            3850..4734
                     /locus_tag="JN331_003616"
     CDS             3850..4734
                     /locus_tag="JN331_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAY7261644.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(4766..5965)
                     /gene="tet(A)"
                     /locus_tag="JN331_003617"
     CDS             complement(4766..5965)
                     /gene="tet(A)"
                     /locus_tag="JN331_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAY7261645.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            6071..6721
                     /gene="tetR(A)"
                     /locus_tag="JN331_003618"
     CDS             6071..6721
                     /gene="tetR(A)"
                     /locus_tag="JN331_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAY7261646.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(6753..6995)
                     /locus_tag="JN331_003619"
     CDS             complement(6753..6995)
                     /locus_tag="JN331_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAY7261647.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLMRWKGGGDVVYMALL"
BASE COUNT         1302 a         2149 c         2213 g         1341 t
ORIGIN      
        1 acgccttcag tagctgagca ggagggacaa ctgatggaga tggaagccac gggagcacct
       61 caaaaccacc atcatacact aaatcagtaa gttggcagca tcaccgtaat cgtacagcta
      121 agaacaagtg cttcaagtct acttattaaa atgtaccttt gaaaaagtag actttctgcg
      181 gtacgctctg acagtaagtt tgcccccgat tttttacgca gcgcgccgga cggagcccga
      241 catgatcctg attcccgatg acgaaccgga gctgagtctg cccgcagcca gtgaggaatt
      301 cctgccggcg ctgtcaggtg agaatgcccc ggtcagcccg gcccgggcct acctgctttc
      361 actcaattcc caccgcagcc ggcagaccat ggcctcgttc ctgaacatcg tcgccgttat
      421 gctcggagct gcctccctgg agtcctgcag ctggggcagc ctgcggcgcc atcacgtgat
      481 ggccgtgacc gaactgctgc gcgacaccgg ccgggccacc gccaccgtca acacctatct
      541 ttcggcactc aagggcgtgg cgaaggaagc ctggatgctc cggctcatgg atgtggagag
      601 cttccagcat atccgggcgg tccgtaacct gcgcggcagt cggctgccca gcggtcgggc
      661 gctgccccag ggggagatcc gcgccctgtt tgccgtctgt gaagccgatc gcagctgcct
      721 cggggcgcgg gatgcagcga tgctggcggt cattctcggc tgcggcctgc gacgatccga
      781 agtagtgagc ctggatttgc gtgacgttgt cactcaggac cgtgcgctta gagtgctggg
      841 taagggaaac aaggagcgac tggcatatgt cccggccggt gcctggcagc ggctgcagat
      901 ctggatcgat gagatccggg gcgagacccc gggcccgctg ttcacacgca tccgtcgttt
      961 cggggatgtg actctaaacc ggctaaccga ccaggcggtg taccatatcc tgcaggtgcg
     1021 ccagggtcag gccggcatca cgaaatgttc tccacacgat ctgaggcgaa ccttcgccac
     1081 cgcgatgctg gataacgggg aggatttaat taccgtgaag gatgcgatgg gccacgcgag
     1141 cgtcaccacc acccagcagt atgaccgtcg cggggagcaa cgcctgcagg acgcgcggga
     1201 tagactcaac cttatttagg gctaagtaaa cacaaacaag ggggctgtac cccctctaat
     1261 tttacattaa cacgatactc gacagacttt cccagtcgta gtcagcaacc atcgcccgat
     1321 ggaggctggg ataaatgctg acagcccgac gtgaagcaaa aggctctcgc cgctggcatg
     1381 gcttaatgtc gcggtcccct caatcactaa cacaatttca gcgctggatg tcattaccgt
     1441 ttgattgttc accgcagtga aaatactgaa atggaaatcc tcaacaggaa taggatagcg
     1501 cgcttctgcg gcgtgctgtt ccggtaacat aggggagccc gcagaattcg gaaaaaatcg
     1561 tacgctaagg ttttccgggc atccgtaagg gccgaaactt cccgtcttcc agtctgcggc
     1621 tctgccgcca gacgtaatcg ccggttaggt tgatgtgctc ccagcccagc ggcgacagga
     1681 attgcagcag ctcgccgtcc accggcttgc cggcctcgac caacccctgg gtggcgcgtt
     1741 ccaggtacac cgtgttccac agcacgatag ccgccgtcac caggttgagg ccgctggccc
     1801 ggtagcgctg ctgctcgaag ctccgatccc tgatttcccc aaggcggttg aagaacaccg
     1861 ccctggccag cgagttgcgc gcctcacctt tgttcaggcc ggcatgcacg cggcggcgca
     1921 gttcaacact ttgcagccag tccaggatga acagcgtgcg ctcgatccgg cccagctcgc
     1981 gcagggccac ggccagtccg ttctggcgcg ggtagctgcc gagcttgcgc agcatcagcg
     2041 aggcggtgac ggtgccctgc ttgatcgagc tggccaggcg caggatgtcg tcccagtggg
     2101 cacgcacgtg cttgatgttc agggtgccgc cgatcagcgg gcgcaacgtc gggtaggctt
     2161 gcacgccctg cggcacgtac agcttggttt cgccgaggtc gcggatgcgc ggcgcgaagc
     2221 ggaagcctag caggtgcatc agggcaaaga cgtgatcggt gaagccggcc gtgtcggtgt
     2281 agtgctcctc gatccgcagg tcggactcgt ggtacagcag gccgtcgagc acataggtgg
     2341 aatcgcggac gccgacattc accacgcggg tgctgaacgg cgcgtactgg tcggagatat
     2401 gggtatagaa cagccgtccc ggctcgctac cgtacttcgg gttgacgtgc ccggtgctct
     2461 cgccccggcc acccgcgcgg aagcgctggc catcggagga tgaggtcgtg ccgtcgcccc
     2521 agtgggcggc aaaggcgtgg cgatactggt ggttgaccag ctcggccaag gccgccgaat
     2581 aggtttcgtc gcggatgtgc caggcttgca gccaggacag cttggcgtag gtcaggccgg
     2641 ggctcgactc ggccatcttg gtcagcccga ggttgatcgc atcaccgagg attgcggaca
     2701 gcagcaacgt cctgtctttg gcctcggccc cgtccttcaa gtgggtgaag tggcggctga
     2761 agcccgtcca gtcgtccacg tccatcagca gttcggtgat cttgatgcgc ggcagtaact
     2821 ggctggtttg gtcgatcagc gcctgcgccc gatccggcac cgccgcatcc agcggggtga
     2881 ttttcagccc tgactcggtg aggatggcat cgggcagctc gttgtccttg gccaggcggg
     2941 tgacggtggc caactgctcg tccagcagct gcaaacgctc ttccaggtac tggtcgctgt
     3001 tcgggttgat cgccaggggc agggcctgct cgcgcttgag tgcggcgaac ttctcggccg
     3061 gcagcaggta gtcgtcgaag tcgcggaact gccgcgagcc cttgacccag atgtcgccgg
     3121 agcgcagggc gttcttcagc tcggacaggg cgcagatttc gtagaatttc cggtcgaggc
     3181 cttccggggt gatcaccagc ggcttccagc gcggcttgat gaaggccgtg ggtgcatcgg
     3241 ccggcacctt gcgcaggttg tcggcgttca tctcacgcag ggtctgcacg gctgccagca
     3301 cgccttgcgc ggccggcccg tgctgttccc caacttttcg ctcggcgaag agtatgaaca
     3361 tgcgccgccg gcgacgaatc gccaaatctc accgtatctc ccgagcggac gatttcgcac
     3421 cggtctgccc gtcgaagggc ttgcgatcga acggggcgac cttttctatg catgtccgcg
     3481 agccagcgtc ttttatggca cggcgctcga cgccgacctt cggacgcgcg gcgtaagcac
     3541 gcttgtcatg gccgggataa gcaccaccgg cgttgttctt tcaagcgtcg cctgggctag
     3601 tgatgcggac tacgacgtgc gtttggtcca ggactgctgc tacgacccgg atcgggatgc
     3661 ccacgaagct ctgttgcgtt ccgggttcgg cggacgtgta caggtcgtgt aattttcggg
     3721 cctcgcatga tcgcggccat agccgcaggt ggattggtgc gaggcagggc cagtccaagt
     3781 cagggtgcgg tcatcgcggc gcctgcaact ctcaaaaacc catcgccgta gcggaggtct
     3841 tgtaatcgca tgtcattacg cacgcccgat ttattgttca cagcgatagc acctgccatt
     3901 tggggcagca cctacattgt caccacccaa tacctgccga acttctcacc gatgacggtc
     3961 gcgatgctgc gggcgttgcc ggcgggttta ttgctcgtga tgatcgtccg acagattcca
     4021 acgggaatct ggtggatgcg catcttcatc ctcggcgcac ttaatatttc gctattctgg
     4081 agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg tcgcggcgac ggtaggcgct
     4141 gtgcagccgc tgatggtcgt gttcatctct gccgctctgc taggtagccc gatacgattg
     4201 atggcggtcc tgggggctat ttgcggaact gcgggcgtgg cgctgttggt gttgacacca
     4261 aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg cgggggcggt ttccatggcg
     4321 ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc ctctgctcac ctttaccgcc
     4381 tggcaactgg cggccggagg acttctgctc gttccagtag ctttagtgtt tgatccgcca
     4441 atcccgatgc ctacaggaac caatgttctc ggcctggcgt ggctcggcct gatcggagcg
     4501 ggtttaacct acttcctttg gttccggggg atctcgcgac tcgaacctac agttgtttcc
     4561 ttactgggct ttctcagccc ggggaccgcc gtgttgctag gatggttgtt cttggatcag
     4621 acgctgagtg cgcttcaaat catcggcgtc ctgctcgtga tcgggagtat ctggctgggc
     4681 caacgttcca accgcactcc tagggcgcgt atagcttgcc ggaagtcgcc ttgacccgca
     4741 tggcataggc ctatcgtttc cacgatcagc gatcggctcg ttgccctgcg ccgctccaaa
     4801 gcccgcgacg cagcgccggc aggcagagca agtagagggc agcgcctgca atccatgccc
     4861 acccgttcca cgttgttata gaagccgcat agatcgccgt gaagaggagg ggtccgacga
     4921 tcgaggtcag gctggtgagc gccgccagtg agccttgcag ctgcccctga cgttcctcat
     4981 ccacctgcct ggacaacatt gcttgcagcg ccggcattcc gatgccaccc gaagcaagca
     5041 ggaccatgat cgggaacgcc atccatcccc gtgtcgcgaa ggcaagcagg atgtagcctg
     5101 tgccgtcggc aatcattccg agcatgagtg cccgcctttc gccgagccgg gcggctacag
     5161 ggccggtgat cattgcctgg gcgagtgaat gcagaatgcc aaatgcggca agcgaaatgc
     5221 cgatcgtggt cgcgtcccag tgaaagcgat cctcgccgaa aatgacccaa agcgcggccg
     5281 gcacctgtcc gacaagttgc atgatgaaga agaccgccat cagggcggcg acgacggtca
     5341 tgccccgggc ccaccggaac gaagcgagcg ggttgagagc ctcccggcgt aacggccggc
     5401 gttcgccttt gtgcgactcc ggcaaaagga aacagcccgt caggaaattg aggccgttca
     5461 aggctgccgc ggcgaagaac ggagcgtggg gggagaaacc gcccatcagc ccaccgagca
     5521 caggtcccgc gaccatcccg aacccgaaac aggcgctcat gaagccgaag tgccgcgcgc
     5581 gctcatcgcc atcagtgata tcggcaatat aagcgccggc taccgcccca gtcgccccgg
     5641 tgatgccggc cacgatccgc ccgatataga gaacccaaag gaaaggcgcc gtcgccatga
     5701 tggcgtagtc gacagcagcg ccggccagcg agacgagcaa gaccggccgc cgcccgaaac
     5761 gatccgacag cgcgcccagc acaggtgcgc aggcaaattg catcaacgca tacagcgcca
     5821 gcagaatgcc atagtgggcg gtgacgtcgt tcgagtgaac cagatcgcgc aggaggcccg
     5881 gcagcaccgg cataatcagg ccgatgccga cagcgtcgag cgcgacagtg ctcagaatta
     5941 cgatcagggg tctgttgggt ttcacgtctg gcctccggac cagcctccgc tggtccgatt
     6001 gaacgcgcgg attctttatc actgataagt tggtggacat attatgttta tcagtgataa
     6061 agtgtcaagc atgacaaagt tgcagccgaa tacagtgatc cgtgccgccc tggacctgtt
     6121 gaacgaggtc ggcgtagacg gtctgacgac acgcaaactg gcggaacggt tgggggttca
     6181 gcagccggcg ctttactggc acttcaggaa caagcgggcg ctgctcgacg cactggccga
     6241 agccatgctg gcggagaatc atacgcattc ggtgccgaga gccgacgacg actggcgctc
     6301 atttctgatc gggaatgccc gcagcttcag gcaggcgctg ctcgcctacc gcgatggcgc
     6361 gcgcatccat gccggcacgc gaccgggcgc accgcagatg gaaacggccg acgcgcagct
     6421 tcgcttcctc tgcgaggcgg gtttttcggc cggggacgcc gtcaatgcgc tgatgacaat
     6481 cagctacttc actgttgggg ccgtgcttga ggagcaggcc ggcgacagcg atgccggcga
     6541 gcgcggcggc accgttgaac aggctccgct ctcgccgctg ttgcgggccg cgatagacgc
     6601 cttcgacgaa gccggtccgg acgcagcgtt cgagcaggga ctcgcggtga ttgtcgatgg
     6661 attggcgaaa aggaggctcg ttgtcaggaa cgttgaagga ccgagaaagg gtgacgattg
     6721 atcaggaccg ctgccggagc gcaacccact cactacagca gagccatgta gacaacatcc
     6781 cctccccctt tccaccgcat cagacgcccg tagcagcccg ctacgggctt tttcatgccc
     6841 tgccctagcg tccaagcctc acggccgcgc tcggcctctc tggcggcctt ctggcgctcc
     6901 tgctgcggcg tccgctcgtg ggccgtggcg cgggtccgcg cgccggcctc gtgcgcctgg
     6961 cgctcgcggg cgaggtccag ggcggccgtc ttcacgttct gcctt
//
LOCUS       DADLSB010000185         6964 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.185, whole genome shotgun sequence.
ACCESSION   DADLSB010000185 DADLSB010000000
VERSION     DADLSB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.185"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..748)
                     /locus_tag="JN331_003620"
     CDS             complement(<1..748)
                     /locus_tag="JN331_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HAY7261648.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLM"
     gene            complement(835..1196)
                     /locus_tag="JN331_003621"
                     /pseudo
     CDS             complement(835..1196)
                     /locus_tag="JN331_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1536..2090)
                     /locus_tag="JN331_003622"
                     /pseudo
     CDS             complement(1536..2090)
                     /locus_tag="JN331_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2233..2355
                     /locus_tag="JN331_003623"
                     /pseudo
     CDS             2233..2355
                     /locus_tag="JN331_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2617..3183)
                     /locus_tag="JN331_003624"
     CDS             complement(2617..3183)
                     /locus_tag="JN331_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261649.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3465..3779)
                     /locus_tag="JN331_003625"
     CDS             complement(3465..3779)
                     /locus_tag="JN331_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261650.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3857..3967)
                     /locus_tag="JN331_003626"
                     /pseudo
     CDS             complement(3857..3967)
                     /locus_tag="JN331_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4291..4968)
                     /gene="ospD1"
                     /locus_tag="JN331_003627"
     CDS             complement(4291..4968)
                     /gene="ospD1"
                     /locus_tag="JN331_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY7261651.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMISNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(5339..5476)
                     /locus_tag="JN331_003628"
                     /pseudo
     CDS             complement(5339..5476)
                     /locus_tag="JN331_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5558..5856)
                     /locus_tag="JN331_003629"
                     /pseudo
     CDS             complement(5558..5856)
                     /locus_tag="JN331_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..6604)
                     /locus_tag="JN331_003630"
                     /pseudo
     CDS             complement(6445..6604)
                     /locus_tag="JN331_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6608..6964)
                     /locus_tag="JN331_003631"
                     /pseudo
     CDS             complement(6608..6964)
                     /locus_tag="JN331_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1852 a         1565 c         1461 g         2086 t
ORIGIN      
        1 tcattagtgg gatgtaaaaa ctgttacggg tgtactgcac catccgttcc accttacctt
       61 tagtctgtgc cctgaagggg cgacacagtc ggggagagaa gcccatctcc ttgccgaact
      121 gccacagcga aggatggaac cggtgctgac cggtctgata tgcgtcacgt tgcagaacca
      181 cagttttcat attgtcatac aacacttcgc gcggcacacc accaaagaag cggaacgcat
      241 tacgatggca ggtctccagc gtgtcataac gcatattgtc agtgaattcg atgtacaaca
      301 ttcggctgta tccgggaaca gcaacgaaca cgtgaagcgg tgagcgacca ttacgcatag
      361 tgccccagtc aacctgcatc tgtcgtccgg gttcagtttc gaaccgaacg gcaggctcct
      421 gctcctgagg aaccgagaga gaacgaatga atgccctgag aatggtcatt ccgccacgat
      481 atccctggtc tctgatctcg cgagcgatta ccgttgccgg gattttgtaa ggatgagcat
      541 cggcgatgcg ttgacgaata taatcccggt attcatccag gagtgaagca acagcaggtc
      601 gcggcgtata ttttggcggc tcagattttg cctgcaaata acgtttaacg gtattgcggg
      661 agagccccag ttctctggca atcgcccggc tactcattcc ctgcttgtgc aggattttaa
      721 tttccataac tgtctcaaaa gtgaccataa gctctcctga atcaggagag cagattaccc
      781 cctggatctg atttcaggcg ttgggtgtgg atcactattg caccgttcgt gacagtgttg
      841 ttgcgccttg ctgccaacga agctccactg cccatcaagt tcacagataa gggcaacatc
      901 agcgtgagcg actggcgcag atgttatccg ccggggcggg agctttttaa tgtccgtatg
      961 acggtattga tgccgatttt cagcgtacgg gcagtatcgc gaactccggc accgttgaaa
     1021 gccatttcag tgatttggtt tttaactccc ggtttacggg cctcgtaaca gtaagtgagc
     1081 tgaaacaccc ggtgacagtc acggcaacgg aatcgatcat gtccttttgg gttctgcccg
     1141 tgacggtaaa cctgaacgga ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa
     1201 gaaccttaaa agcgggtatt ataaccccta attcaactaa ttggaggcat tgtcattttt
     1261 aatctatgga tacacttaaa aatcctctca ccttacgtta aaaaccttct catgtttaga
     1321 gttttttatt atgtttatca taatgatatg acgatctgag tttttaaaat caagaagaaa
     1381 agataagttt tttaaatgca cagtgcctgt ggataacttt gcgtaaatcc ggaggacaga
     1441 tcactctgaa gcaagacaac agcatctgaa aacaatatgc tcacgtttca accacagggc
     1501 gtaagtgatc ctacccacgt aatatggaca caggcctaag cgaggttctg gttttcaaat
     1561 tgttccggac tgaggccgcc acaccaactg tgccgtcgcc agcgattgta atcacattcg
     1621 atataattaa acactgttgc ccgcattatt tcccggctga taaagtgttc tccgtggaga
     1681 cattccactt tcagcgaatg aaagaagctt tccacgcagg cattatcgta gcagcagccc
     1741 tttgcgctca tactgccacg cagattatgc cacttcagca gcgcctgata atctgctgaa
     1801 cagtactgac ttcctcggtc cgagtgaacg ataacgttct ggggtctctt acgccgccac
     1861 agcgccatct gcagggcatc gcaggccagt tgcgccgtca ggcgtggtga catggaccag
     1921 ccaataacgg cacgtgacca caggtcaatg accacagcca gatacagcta gccttcatct
     1981 gtacgtaagt acgtgatgtc tcccgcccac ttctggttcg gaccactggc gtaaaaatcc
     2041 tgctccagca gattttctga cacaggcagg ccatgtgcgc ggtaactgac cgtattgctg
     2101 ggtaagatca atttgctcac tggtatttac tacgtgcatc gaatgagtga tcaagagccg
     2161 tatgacgcga gagtgtcacg tacggttccg tgagcagccg gagagtgaaa ctctctccgg
     2221 ctgactcgac tcgcaggttc cgacagcggt gacgaacatg cagcggtgtt gttctcgctg
     2281 atcggcacat gccgtctgaa caatgtggag ccagaaaaat ggctgcgtta cgttattgaa
     2341 catatccagg actgaccggc aaaccgggta cgcgatctgt tgccttggaa agttgatctg
     2401 acttctcagt aaatatcaat atgattctga cgagccgctt acctttgagc agagaaaggg
     2461 gagagtgatc ccacatccat gagctaggaa aatacaaaaa ctggctatac aacaattcaa
     2521 tatattagga cttacttgcg aggagaaata tagcaccgct caggcaattc ctgggtattt
     2581 acttttctta gtttctttac tcgggatgtt ctgtcatcag aaaggcgatt ctaaatttgt
     2641 aatatagtca ctcatattcc cacgtaattt ctttagatca atatattgca catttttctc
     2701 aaaaaactga tcggaaataa gatttgcaac attgcttgtt gtatcatttt taacgcttct
     2761 attcattcct tcacaaagtt cgtccatttt ctttttgaaa taactgcttc ctgatacaag
     2821 atgatttatg gaactttgtg cagcattgat gtctagagaa atgcgataat ctttctcaac
     2881 ctgtttaaat atctgtttac ggagattatc gccgattttt cggctgtgtt ctgcacaaaa
     2941 cttatcaata cattggttta ccacccggga tacgtagact tttttttcat catttttcca
     3001 atctgtaata ttttcaccga tttgttctga aacagagctc aaaatactag tatttatttt
     3061 tgttgatgat atttttacta tccttttccc cattgtacag cggataatct catccacctt
     3121 tcctgaaaaa tatcttttgt gagataaact gattccaaaa ttattaaaag atgttccaag
     3181 cattataatc actttgtcat gtaaaatagt aacattgtca ttattttagc tgattcaaac
     3241 aattctgtaa atgctttgag ttctttactc ccttggctca atgatggacc gtctggaatc
     3301 ccagagacta aactctctga gaaagagagt atgaaaaaac ttggggcagt ccctaaatga
     3361 agggaccaac agcggtaaaa taaccggcat cattcataat gatgccctgt tagttgtagt
     3421 tatgaagatt tacgtcatta aaattgctgt tcgtagtgtc agtcctatgg tctggcatca
     3481 gctgaggatc gtagccgata catcactggc ttcacgacat tcgtgttgag gctattcatg
     3541 aaaactccac gctgaaaacg ccgttttgta taaacggtca cggtatgccc ggagccactg
     3601 ttgctgatga attcgataaa accttggcgt ttcttgaagc tatcgttaat gcggataatg
     3661 aaacaacgat tggtgagatt cgctcttttg ccgacgccct ggacgctgtc cggttcaatc
     3721 gcaacaaaat caaccggcag ctgagcaaac ccaaccttgc ctctctggcg ctggagcatg
     3781 aagtgatttg gctgggacgt agccgctgac agcttctata aaagtatgat cctacccacg
     3841 taatatgaac acaggcttaa gtgaggttct ggttttcaaa ttgttcagga ctgagacctc
     3901 cacaccaact gtgccgtcgt cagcgattgt aatcgcactt gatataatta aacactgtat
     3961 atttcatttg tggagtctcc agtctgaggg ggctttatgc attccgtatt gggcactatg
     4021 atgccagaaa tctgcaaggc tccacacctt cctgtatttc ccttcctcta tcacagtgca
     4081 ctttacttaa tagggtggga tgtgttcatt acatttattg tgttttttat gattgttttt
     4141 gcgtccagtg tcgttcgtca tgggaaactg gttctatggg tagtatcgct cagtctagtt
     4201 aactgtttgt ttctttttag aaattaagca gatcacacaa ttcggggaga gatccttcta
     4261 agtgcaattc tttgcagcta tttagcacac ttaaaacaac tcttgtattt tgtcagaaat
     4321 aatattaaac atgtcttgtt cattattgtt aagtctgtct gctatttctg taagttttat
     4381 cccactatga tggcatatat ttatatatgc catcatagcc tcgatattcc ctttccctgc
     4441 ggccagaaat aatcctggta acccctcatt atctcttaca tccaatagtt taatcgtttc
     4501 ttttggtgat aaatcaaaag ttttaataaa atctccataa gcttgaatcg cagcggaatg
     4561 accttcttga agagctatga acaaagcaga ttccccagca ttatttcgtg ctgcaagaac
     4621 ttctagtttt tcctctttgg gtatttcggc tgccaacagc gattcaccgt atttacgcgc
     4681 tgcatcggca tttccatgcc aaatcaatga aaacaaatta tggcggttat actccttatt
     4741 attctttgtc ggaatggata tgctctgtat tttggccaat tttctgaaag aaacaccact
     4801 atatcccccc cctgattttt cttcattgat ggcgtgggat atagcggtat ttgtcacgtt
     4861 aatgatatta cttatcattc ctttattttc attagcagta ttgctgccta ttattaggtg
     4921 catatttttg ttgtttgctg gatgtaatcc atagttattt attgacattt ttgttcatcc
     4981 tttttgctaa aaaaaacaat aacctatttt tgtaatgtaa tagatattag tctattgcta
     5041 atctaaaaaa accattgcca cattgaatgg ctatattcac caataaattc tttcgatttc
     5101 gtatattgac atatcgtaga tatatcttat ccctaagatt caataatcga gtccgtttac
     5161 cattctgtgc aagcgccttc actcagaagt gaccgtcaag gcgatcacca aacttgataa
     5221 taaagcagct cattgccata cgccagtcct taaggggcgt tgtccatttc tgtgatgctg
     5281 cctagattta actgccagct ccggcttatc tacatctaaa tatgtgagcc taataccgct
     5341 acactttggc cagactatgt ttttctccgg gaagtgggct aaccgtctcc ataaagtgac
     5401 gaactttttt caacagtggc catattgaac ggcactgatg attacgggtt attgtctcat
     5461 gaagagccgg ccacatcact gacagcagtg acttcaggcc ttcagcacca gacagcctga
     5521 accagttaat ccatcgcccg acagatttag taatctaggg ctggtttttc ttcagctgtt
     5581 gcggttcgaa agtgccatta cgatcacgca gagtacggat ttccagaggt tcatctccag
     5641 tagtgataat cttcgttaag caaccattgc gggaattgct gccagatcta agctgatttt
     5701 tctcgcaccc aagatgatga gtcatattag cattgagtac ggtttcattt tcagcatcca
     5761 atcaaactga ctgagatctt caggattttt gagacttttg gctagttcat tagctaaagc
     5821 ccgcaactta ttagatgtca actgtttttc attcataaat tcaccttcat ttgatatcaa
     5881 attaaatagt tcaacatcag agaattatac aaacttatgt acaggctcct atgatcttcg
     5941 aatcctgaag attatagtga aaataagttt tcacgttgaa atcccacata gttagtatat
     6001 gataaataat catggatgac ctgattaacc tcttggggta gtgcttttat agaagaataa
     6061 atattctact aatttaatac atcacctaaa atagtattgt tttttatggt tggttgaagg
     6121 tcgtggtgat gatgttagtc cttttgagaa tattccagtg ccagatctta cttataataa
     6181 aggggcttgg cagtttggat aaactattgt tcttattcca ctaatacaaa ctttttgtaa
     6241 agtatattaa atatacttca tttcatactg aaatccccgg aagtattcaa tcaaacaatt
     6301 acaccataaa aggaagaggg ggccctaatt aagttgtcaa gcatgtttat gatacctgca
     6361 gggtataaat tttctggctg caagcagaca catataaaac gtctttattg atcctaccca
     6421 cataatatgg acacagtcgt aagtgaggct ctggttttca aattgttccg gactgagacc
     6481 accacaccaa cggtgccgtc gccagcgatt gtaatcgcac tcgatataac taaacactgt
     6541 tgcccgcact atttcccgat ggataatagt gttctccgtg gagacattcc attttcagcg
     6601 aatgaatttt catctgttgc tgtatccagt catacagtga ctgcatcagt ggcgcggctc
     6661 tggcttttct tgccgccaga cgctgttctg ctgaacatcc ccggacttct gcctcgatgg
     6721 catacagttc accgatacgc tgcagggctt ccgtggtgat gtcggttggc actcttgcat
     6781 gcacatcgtg gatttttctc cggacatgag ccatacatgc ggcttccgtt attctgccgg
     6841 attcgtacaa cgcccggtaa ccaccgtaaa catcggcctg aagcactccg ctgtaaccgg
     6901 ccagatgatt ctgtggatgg atacctttcc ggtccggtga gtacgcgaac cagaccgccg
     6961 gagg
//
LOCUS       DADLSB010000186         6912 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.186, whole genome shotgun sequence.
ACCESSION   DADLSB010000186 DADLSB010000000
VERSION     DADLSB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.186"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..207)
                     /locus_tag="JN331_003632"
     CDS             complement(<1..207)
                     /locus_tag="JN331_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261652.1"
                     /translation="MNMLRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQS"
     gene            727..1422
                     /gene="minC"
                     /locus_tag="JN331_003633"
     CDS             727..1422
                     /gene="minC"
                     /locus_tag="JN331_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY7261653.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1446..2258
                     /gene="minD"
                     /locus_tag="JN331_003634"
     CDS             1446..2258
                     /gene="minD"
                     /locus_tag="JN331_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY7261654.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2262..2528
                     /gene="minE"
                     /locus_tag="JN331_003635"
     CDS             2262..2528
                     /gene="minE"
                     /locus_tag="JN331_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY7261655.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2900..3244)
                     /locus_tag="JN331_003636"
                     /pseudo
     CDS             complement(2900..3244)
                     /locus_tag="JN331_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3279..3398)
                     /locus_tag="JN331_003637"
     CDS             complement(3279..3398)
                     /locus_tag="JN331_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7261656.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3359..3544)
                     /locus_tag="JN331_003638"
     CDS             complement(3359..3544)
                     /locus_tag="JN331_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261657.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3645..3818
                     /locus_tag="JN331_003639"
     CDS             3645..3818
                     /locus_tag="JN331_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261658.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3880..4164
                     /locus_tag="JN331_003640"
     CDS             3880..4164
                     /locus_tag="JN331_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY7261659.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4168..4503
                     /locus_tag="JN331_003641"
     CDS             4168..4503
                     /locus_tag="JN331_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY7261660.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4560..6881)
                     /locus_tag="JN331_003642"
     CDS             complement(4560..6881)
                     /locus_tag="JN331_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7261661.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT         1868 a         1641 c         1567 g         1836 t
ORIGIN      
        1 tgattgtaag ttttcagcgg ctttttcgcc gagatatttc cttgttaact ctggggaaat
       61 gccatctgag ttcacacaaa tatacgggtc gcaaacaacg ccgcacgccg gagattgaag
      121 tccgccagcg tcatgtccag acgccattga tgagaacatc atccccacac ctgacaatcc
      181 gatataaata atacgcaaca tattcatagc acatcctgat aatccaaccg tacaacactt
      241 atgctcattt cgtcatcaat ccgtaatcaa aaccgctgac ctggacgagt tacgatgacc
      301 tgacagaagt atcagacatc ttctgcggtt tctatgatag agcaattcta tggcggatat
      361 taagataacg ggtatctgtt ctttaatgat gctgcaaaaa atggaaagtt aagaaagcct
      421 ggcgtggtga aaaaatacac ttgcctcaat actggatatt taaacaggtc tggagatgct
      481 gtcacgatta gggtgcggaa aaaggaagcg taaataagaa gaatcaaaag tacgacggca
      541 atgggttgat tgacaagggt attttttaag ctatgaatca gcgccattta tcacagaata
      601 gacttttact ctgaataaat gggagggtga cttgcctcaa tataatccag actataacat
      661 gccttatagt cttcggaaca tcatcgcgcg ctggcgatga ttaatagcta attgagtaag
      721 gccaggatgt caaacacgcc aatcgagctt aaaggcagta gcttcacttt atctgtggtt
      781 catctgcatg aggcagaacc taaggttatc catcaggcgc tggaagacaa aatcgctcag
      841 gcccccacat ttttaaaaca tgcccccgtt gtactcaacg tcagtgcact ggaagacccg
      901 gtaaactggt cagcgatgca taaggcggtt tcggcaaccg gtttgcgggt tattggcgta
      961 agtggctgca aagatgcgca acttaaagcc gaaattgaaa aaatggggct gcctatcctg
     1021 acggaaggaa aagaaaaagc gccacgtcca gctcccgcac cgcaggctcc agcgcaaaat
     1081 acaatgccgg tcacaaaaac gcgtttaata gataccccgg tgcgttccgg tcagcgtatt
     1141 tatgctccac aatgtgatct gattgttaca agccacgtta gcgctggggc cgaattgatt
     1201 gccgatggga acattcatgt ctatggcatg atgcgcggtc gtgcgctggc aggggcaagt
     1261 ggtgaccggg aaacgcaaat attttgtacg aacctgatgg cggaattggt gtccatcgca
     1321 ggtgaatact ggctgagtga tcaaatccca gcagaatttt atggcaaagc ggcgcgactg
     1381 cagttagtcg aaaacgcttt gaccgttcaa ccgttaaatt gatccctttt tgacaaggaa
     1441 tttctatggc acgcattatt gttgttactt cgggcaaagg gggtgttggt aagacaacct
     1501 ccagcgcggc catcgccact ggtttggccc agaagggaaa gaaaactgtc gtgatagatt
     1561 ttgatatcgg cctgcgtaat ctcgacctga ttatgggttg cgaacgccgg gtcgtttacg
     1621 atttcgtcaa cgtcattcag ggcgatgcaa cgctaaatca ggcgttaatt aaagataagc
     1681 gtactgaaaa tctctatatt cttccggcat cgcaaacacg cgataaagat gccctcaccc
     1741 gtgaaggggt cgccaaagtt cttgatgatc tgaaagcgat ggattttgaa tttatcgttt
     1801 gtgactcccc ggcagggatt gaaaccggtg cgttaatggc actctatttt gcagacgaag
     1861 ccattattac caccaacccg gaagtctcct cagtacgcga ctctgaccgt attttaggca
     1921 ttctggcgtc aaaatcacgc cgcgcagaaa atggcgaaga gcctattaaa gagcacctgc
     1981 tgttaacgcg ctataaccca ggccgcgtaa gcagaggtga catgctgagc atggaagatg
     2041 tgctggagat cctgcgcatc aaactcgtcg gcgtgatccc agaggatcaa tcagtattgc
     2101 gcgcctctaa ccagggtgaa ccggtcattc tcgacattaa cgccgatgcg ggtaaagcct
     2161 acgcagatac cgtagaacgt ctgttgggag aagaacgtcc tttccgcttc attgaagaag
     2221 agaagaaagg cttcctcaaa cgcttgttcg gaggataagt tatggcatta ctcgatttct
     2281 ttctctcgcg gaagaagaac acagccaaca ttgcaaaaga acggctgcag attattgttg
     2341 ctgaacgccg tcgcagcgat gcagaaccgc attatctgcc gcagttgcgt aaagatattc
     2401 ttgaggtcat ttgtaaatac gtacaaattg atcctgagat ggtaaccgta cagcttgagc
     2461 aaaaagatgg cgatatttct attcttgagc tgaacgtgac cttaccggaa gcagaagagc
     2521 tgaaataagc ccgctgtaaa aacgcattta tcttcaaggc agaatcattt ctgccttgag
     2581 tttttcatcc ctctcatcca cgttgtggta aagcggcgag tattcttgct gatactcctc
     2641 attgctattt ccacctcccc ctttacacct taaggctgtt cccacgcaca gaacaaaatg
     2701 tttatgcctg gttgagtaaa taaccatgtt gttagtgtgg atatactggg agatgataac
     2761 cccgcaattt cattattcat tgttctccca taggatgagg tttcccgccg taactggcgg
     2821 gctttttttg ccccagaatt ttctctttca acatcctgta aacgaaaact gcgccgaagc
     2881 gcagtttaat cgttgatggt cagaacgacc agttcacacc agccaccgcg ttccacgggg
     2941 actccacacc ggcaccatgg ctatacccca ccccgaaatg cccgcttaac gtactgctga
     3001 atgaggcttt aatacctgcc tggtatattc cacgtctgcc cgacaaatca ttgacaaaat
     3061 taccgtcact attcactttc acccgattat catcgacaaa ttctttgcgc acagccgcct
     3121 tcaggcaggg ttcaatttcc ataccgttcc ccagacgcat gttgtaactc agcgttgcac
     3181 ccagttcacg atatatactg cgggtatcga ctgatttcga ttccatgcca ttggataaat
     3241 gatagtagcg tctaagtgac ctaagctctg ctgcaacatc agcccgcagg caccagacca
     3301 ggggattcat cctgaagaga cagcgcaggt gtattgtgtt caccgctcac caaagacatc
     3361 atgatgaaat gatgatatcc cgcataagaa agaggcattt tttacacgca gtgcgctgaa
     3421 gtgtagttgg ataaaaaatc actcttttca gataatactg tcgtacgctt atctttagac
     3481 aatgaggccg tcggcaaaat aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc
     3541 tcacataaaa atcacacaat aatattgata aatcagatta tcttaccttt acaacattgc
     3601 gttaacagtc aatgacactt cttgaataaa aatattgtca atcaatgagt tattcctcat
     3661 ttaagattat tctcattcac gtaaaaaaca taattcctat catcacagcc acgctcattt
     3721 tgaattatct gaataatagc gagcgttctt tagttaagca gatcttaatt gaagacgaaa
     3781 ttattgtgtg tgcaacttac ttaataccgg acatctaagt tgaagaaaaa aatacttgcc
     3841 ttcgggctta tctcagcatt gttctgctcc acgccagcga tggcggatat gaaccgcacc
     3901 acgaaaggtg cattgctagg cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc
     3961 gtactgaaag gtgcagctgt tggcgctggt gttggtgcag taacagaaaa aggccgcgac
     4021 ggtaaaaatg cgcgtaaagg tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt
     4081 ctgaccggca atggcctcga aggggcgatt aaaggtgccg tgattggcgg tacgggtggt
     4141 gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt actggcgggc
     4201 ctgtttgttt tcgcaccaat gacctgggcg caggactaca atattaaaaa cggtttgccg
     4261 tcagaaacct atattacttg cgcagaagcc aacgaaatgg caaaaactga cagcgcacag
     4321 gttgcagaaa ttgttgcggt aatgggtaat gccagcgttg ccagccgtga tttaaaaatt
     4381 gagcaatctc cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa
     4441 gacccgcaaa tgctgttgat cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa
     4501 taatatttca tatgtaccgc gtcgatgaat acgacgcggt tttttagcta acggcgggat
     4561 cagaacttat atttcaggcc caccatcact gcggtgtcat tgtagccatt atcacccagc
     4621 tgcacgccca cattccccca gacgctggcc gctggattca ggttgccatt tacgccaagt
     4681 ttaatttccc ccaaattact caccccatct tgttcgactt tcacaccgtt cattgagaca
     4741 gcaaaatctt tactgttatg gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg
     4801 tgcggaccgc tgtttttctc ctgagtacga ataaaggtgc ggaagccgag tcgggtctgg
     4861 atgttgttat ttgcgtcatt ctcaatacgt gagccattgg cttccgtgtg cgcattatga
     4921 tcgacgccca tccagatagc ctgtgcctgt ggctggaaga tccacgtata tttcgccgct
     4981 tccagaccaa aggatttatt cagggcaaaa ccgtaacccg cttccagcga gacggttgca
     5041 cctttcgcgc tataggattc ataggctaat tcatcccctt tcacggagtt tttaaaccag
     5101 ctatattgcg cccaggagtc aatgtatgcg ccttttttac tgatgtcatc ggcaaaccag
     5161 gtggcataca gccctgcgct atagcctctg acactccctt tcgaacgata atccgacacg
     5221 ctggaatgag ttaagttgta gtcgcgggca taaccagcca tcactcccaa acgccaactg
     5281 tcactatcgg taaaaccacc ggttaacaga tcgcccccta actgcgagac gtagcgatgc
     5341 gaggtcgttc tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc aatttgacgt
     5401 agccaaagtc ggcttgaacg ctcctgttca gttacaggat cgatgtagcg cgtttcaccc
     5461 gcacggtcgt tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa
     5521 cttccagctt cggggcgcag gacgggggta ggcgcaacag taggcgctga acttcctccg
     5581 gagtttgttt caccgccgct cgttgcctgg aagttagtca gataccagtt tttattggtc
     5641 gcttcggtat tacctcgggt taatgaatat tcgtaggcac cggcgaaaat cctcgaatcc
     5701 ttaataaatt ccccatttga ttccccctca acgctgataa tctctatccc atttaacgtg
     5761 taagctccgc tgcctcctgc attggtaact ttaactcggg ttgttccaga ggtatttcca
     5821 ttaataacga ctttatccgt agctgaatta tcatcatcca attcagttct gagatgcaat
     5881 acgccattat taccaacgta attttcagtt attgttaatc ttgttggttc gacatcccgt
     5941 ccatctgccc gggaaatata gaccgtacta ttatcaaccg tgaggttgct caccgttgag
     6001 tcagctttaa tatcccaggt actattatct gaaagcgaca gataggtatg agtgttatca
     6061 tctgttgaaa tattagctga acccgttatt tttgaattag ttgcatcgac ggtcaaatga
     6121 cctttaatat tattgataaa aacatctgca gccgatgata cctcactatc cgtaatggtc
     6181 acgttactcg tctcattttt atggtagagg atttcatttg ctgaccgtag tgagctacca
     6241 gataaaatat ctaccgttgc ttcctgacca tttatggaag actccagcac aattccagac
     6301 ccctgatgcg cgactgcaga tgtatttttc agcgtcaccg tgccgccatt ataaatttta
     6361 gcaatcgcgg cgtcaggacc cgttgtataa atttctccct ggttaaatga aactgtcccc
     6421 tggcgggcag cgatggcatg agcattgtcc ccatgcgtgg tgatatcaac atcggttaat
     6481 gtaacgctgg cattggtata atcaagcgtg tcaccagcct tgacgacagt gccattagcg
     6541 tacgcggcat aactttctgt tccgtgggtt gtgatctccc cttttgccat atccagtttt
     6601 gctatggcat tattttcatt acttcctgta tcggcagtac tttcttcatc aagagaagat
     6661 acacacgcac gcgcgcttcc gggagatgca cacgcaagaa cgccgactgc gtgattaccc
     6721 gcagtaacaa taaatgtatc atctccgtca gaaaaagagg gggtaaccga accgtcagag
     6781 ttgactctta ttatggtgtc agtattcagg tttccactcg tttgacttag caaaccaatt
     6841 gagtaatccc ccgttgtgac aatcgtgttt ttaccctgca aaataacagc accgccaaat
     6901 tcacttacga tt
//
LOCUS       DADLSB010000187         6887 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.187, whole genome shotgun sequence.
ACCESSION   DADLSB010000187 DADLSB010000000
VERSION     DADLSB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.187"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            8..892
                     /locus_tag="JN331_003643"
     CDS             8..892
                     /locus_tag="JN331_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAY7261662.1"
                     /translation="MLVTLATGRGLMLVGEPGTAKSLLSELLATSISGDAGLTIQGGA
                     STTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITRTPLEVQDCLL
                     GMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKRRFDFETVFPI
                     MDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEKKTSMDTLTAI
                     MSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRARLRRYFEQRVA
                     THKEAHWQAYYQARHRLP"
     gene            903..3182
                     /locus_tag="JN331_003644"
     CDS             903..3182
                     /locus_tag="JN331_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261663.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGNSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSKKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLAPFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            3175..4311
                     /locus_tag="JN331_003645"
     CDS             3175..4311
                     /locus_tag="JN331_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY7261664.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            4308..6311
                     /locus_tag="JN331_003646"
     CDS             4308..6311
                     /locus_tag="JN331_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HAY7261665.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            6436..6887
                     /locus_tag="JN331_003647"
                     /pseudo
     CDS             6436..6887
                     /locus_tag="JN331_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
BASE COUNT         1603 a         1824 c         1879 g         1581 t
ORIGIN      
        1 cgaacgtatg ttagtcactc ttgccaccgg acgcggtttg atgttggtgg gggaacctgg
       61 caccgcaaaa tctctcctgt ctgaattact ggcaacctct attagcggcg atgccgggtt
      121 aaccatccag ggcggcgcat ctactaccga agatcaaatc aagtacggct ggaactacgc
      181 cctgcttatc aatcatggtc catcaacgga agcgctggtc cctgccccgc tttaccaggg
      241 aatgcgcgac ggcaaaatcg tccgttttga agagataacg cgtacaccgc tggaagtgca
      301 agactgtctg ttaggtatgc tttctgacag ggtgatgacg gtgccggaac tcactggtga
      361 agccagtcag ctctatgcgc gagaaggctt taatattatt gccactgcca atacccgcga
      421 tcgcggcgtc aatgaaatga gtgctgcgct aaagcgtcgc ttcgattttg aaactgtctt
      481 tccgattatg gatttcgccc aggagctgga gcttgtcgcc agtgcttcgg cgcgtttgct
      541 ggcccatagc ggtattccgc ataaagtgcc tgacgcggta ctggagttac tggtccgcac
      601 cttccgcgat ctgcgtgcca acggcgaaaa gaaaacgtca atggatacac taacagcgat
      661 tatgtccacc gctgaagccg tgaacgtggc ccacgccgta ggcgtccgcg cctggttttt
      721 agcgaatcgc gcaggtgagc cagccgacct ggtggactgc attgccggaa ccatcgtcaa
      781 agataacgaa gaagatcgcg ctcgtctgcg ccgttacttc gaacagcgtg tcgccacaca
      841 taaagaagct cactggcagg cttattatca agcccgccac cgcctgccgt gaagaaagat
      901 gcatgagcga gccgttaatt gtcggcatcc ggcatcatag tccggcctgc gcccggctgg
      961 tgaaatcgtt aatcgaaagc cagcggccac gatacgtgtt gattgaaggc ccggctgatt
     1021 ttaatgaccg ggtagacgaa ctgtttttag cccaccagct tccggtagct atttacagtt
     1081 attgccagta tcaggacggt gcagcccccg ggcgtggtgc ctggacgcca tttgctgaat
     1141 tttcgccgga gtggcaggcg ctacaagccg atcgtcgtat tcaggcacaa acttacttca
     1201 tcgatttgcc ttgctgggcg cagagtgaag aagaggacga ttcgcctgat acgcaagagg
     1261 aaagccagac cttgctgctg cgtgccaccc gcatggataa cagcgacacc ctgtgggatc
     1321 acttgttcga agatgaaagc cagcaaactg cattaccctc tgcactggcg cactattttg
     1381 cccaactgcg gggcaactcc cccggcgatg ctctcaatcg tcagcgcgaa gcctttatgg
     1441 cccgctggat tggatgggcg atgcagcaaa ataatggcga cgtgttagtt gtctgcggtg
     1501 gctggcacgc tccggcactg gcaaaaatgt ggcgcgaatg tccgcaggta attaacacgc
     1561 cagaattgcc gtcgctggca gatgccgtta caggttgtta tctcaccccc tacagtaaaa
     1621 agcgccttga tgtgctggca ggataccttt caggaatgcc tgccccggtc tggcaaaact
     1681 ggtgctggca gtggggctta cagcaggcgg gagaacaact gctaaaaacg gttctcaccc
     1741 gtttgcgcca gcacaagttg cctgcttcga ccgcggatat ggctgccgct catctgcatg
     1801 cgatggcact ggcacagttg cgcggtcata cactaccgtt acgcactgac tggctggatg
     1861 ccatagcagg atcgctgatt aaagaagccc tgaatgcgcc gttgccgtgg agctatcgcg
     1921 gcgttattca tcccgatacc gatccgattc tgctaacgtt gatagacaca ttagcgggtg
     1981 acggattcgg taaacttgcc ccttctacgc cacaaccgcc tctgccaaaa gatgtcactt
     2041 gcgaactgga acgtaccgca atctctcttc cggcggagct taccttaaat cgctttaacc
     2101 ccaatggact agcgcaaagt caggtgttac atcggctggc aatactggag atcccaggga
     2161 ttgtacgcca gcagggaagc acactgacac ttgcaggcaa cggtgaagaa cgctggaaat
     2221 taacccgccc gcttagccag catgcggcat tgattgaggc cgcctgcttt ggtgccacac
     2281 tccaggaagc cgcacgccat aaattagaag ccgatatgct ggacgcgggt ggaatcggca
     2341 gcattaccac atgtcttagc caggcggcgt cagcgggtct ggcgcccttc agtcaacaat
     2401 tactggagca actcacatta ttaatcgccc aggaaaatca atttgccgaa atgggccagg
     2461 cgctggaagt gctatatgcc ttatggcggc tggatgaaat tagcggtatg caaggcgcgc
     2521 agatattaca aacgacgtta tgcgcggcta tcgatcgcac gctgtggctg tgtgaatcca
     2581 acggcagacc ggatgaaaag gagtttcacg ctcacttgca tagctggcaa gcgttgtgtc
     2641 atatcctgcg cgatctacat agcggtgtgc aactccccgg cgtttcgctt tctgcggcgg
     2701 tagccttgct ggagcgacac agtcaggcga ttcatgtcct ggcactggat cgcggcgcga
     2761 ctcttggtgc gctgatgcgt ctggaacatc ccaacgctag tgccgaagcg gcgctgacga
     2821 tgctggcgca gttatccccg gcacaatccg gcgaggcgct gcacggtttg ctggcattag
     2881 cccgtcatca actggcctgt cagccggcat ttatcgccgg tttcagcagt catttaaatc
     2941 aactaagtga tgccgatttt accaatgccc tgcccgattt acgcgcggca atggcctggc
     3001 taccgccacg agaacgcggg acgctggcgc atcaggtgct tgagcattat caactggcgc
     3061 aacttcccgt ttcggcactg caaatgccgt tgcattgtcc accgcaagcc attgcacatc
     3121 atcaacaact cgaacagcag gcactggcat cgctgcaaca ctggggagtt ttccatgtct
     3181 gaactgaacg atcttctgac cacccgtgag ctacaacgct ggcgattaat tcttggcgaa
     3241 gcggcagaaa cgacgctttg tgggctggat gacaacgccc ggcagataga tcacgcgctg
     3301 gagtggctgt atgggcgcga tcctgaacgg ctccagcgtg gtgaacgctc tagtggatta
     3361 ggtggctcaa atctcaccac tcctgagtgg atcaacagta ttcacacgct gtttccgcaa
     3421 caggtgattg agcggctgga aagcgatgcc gtactgcgct acggcattga agatgtggtg
     3481 acaaatctcg acgtgctgga acgtatgcag ccttccgaaa gcctgctacg cgccgttttg
     3541 cacaccaaac atctgatgaa ccccgaagta ctggctgccg cccgcaggat tgtgcgccag
     3601 gttgtggaag aaattatggc gcgactggca aaggaagttc gtcaggcttt ttctggtgtc
     3661 cgcgatcgtc gtcgccgctc atttattcca ctggcgcgaa actttgattt caaaagtact
     3721 ctgcgcgcca acctgcaaca ctggcacccg caacacggca agttgtatat cgaattcccc
     3781 cgctttaaca gccgcattaa acgccaaagc gagcaatggc aactggtctt actggttgat
     3841 caaagcggat cgatggtcga ttcggtgatc cactctgcgg tgatggcggc ctgtttatgg
     3901 cagttacccg gcattcgtac ccatctggtg gcgtttgaca caagtgtcgt tgatctcacg
     3961 gcagacgttg ccgatccggt agagttatta atgaaagtgc aattaggcgg cggaaccaat
     4021 atcgccagtg ccatggagta tggtcggcaa cttattgaac aacctgcaaa aagcgtcatt
     4081 atcctcgtga gcgattttta tgaagggggt tcatcatcat tgctgacgca ccaagtgaaa
     4141 aagtgtgtcc agagcggcat caaagtgctg ggactggcag cgctcgatag cacagcaaca
     4201 ccttgctatg accgcgatac ggcccaggcg ctggttaatg tcggcgcaca aatagccgcc
     4261 atgacaccgg gcgagctggc atcatggctt gcggagaatc ttcagtcatg aattcactac
     4321 gtccggaatt attagaactg acaccgcagg cactgacggc gttaagcaat gccggttttg
     4381 ttaagcgcag ccttaaggaa ctggaaaatg gcaacgtccc ggaaatcagc catgagaaca
     4441 gcgctttaat cgccaccttc agtgacggtg tccgtaccca gctggcgaac ggccaggcac
     4501 tgaaagaggc tcagtgcacc tgtggggcca gcggcatgtg ccgtcatcgc gtgatgctgg
     4561 tgttaagtta tcaacgactt tgtgctaccg cccagcctac gggaaaagaa gaggaatggg
     4621 atccggcaat ctggctggaa gaactggcta cccttcccga tgccacccgc aaacgcgcac
     4681 aggcgctggt cgctaaaagc atcaccattg agttgttctg tgcgccgggt gaaattccct
     4741 ctgcccgctt accgatgagc gatgtgcgtt tttattcccg cagcagtatt cgtttcgccc
     4801 gttgtgattg tattgaaggc acactttgcg aacatgtcgt actggcagta caggcctttg
     4861 tccaggcaaa agcgcagcaa gcggaattga ctcatttaat ctggcagatg cgcagcgaac
     4921 acgtcacatc atctgacgat ccgtttgcca gcgaagaagg caaaacgtgt cgtcaatatg
     4981 ttcaacaact aagccaggca ttatggctga gcggcatcag ccagccgctt attcactacg
     5041 aggccgcttt cagccgcgcg cagcaagcag cggaacgctg cagctggcga tgggtgagtg
     5101 aatcgctacg gcaactgcgc gccagcgttg atgccttcca cgcccgcgcc agccactatc
     5161 atgccggaga atgcttacgt cagcttgcgg cattaaacag tcgattaaat tgcgcacaag
     5221 agatggcccg gagcgacagt gttggtgaag ttcctcctgt gccgtggcgc acggtcgttg
     5281 gctctggcat tgccggagaa gcaaagcttg atcatctgcg gctggtgtct ttaggtatgc
     5341 gttgctggca ggatattgag cattatggtt tacgcatctg gtttaccgat cccgacaccg
     5401 gcagtatttt gcacctttcg cgcagttggc cgcgaagtga acaggaagac tcaccggcag
     5461 ctacgcgtcg gctgtttagt tttcaggctg gcgcactggc gggtgggcaa attgtttcac
     5521 aagcagcaaa acgcagtgcc gatggcgagc tgctgttagc tacccgcaac cgcttaagca
     5581 gcgttgtgcc gctgtcgcct gatgcctggc aaatgttgag cgcgccgtta cgccagccgg
     5641 gcattgtggc tttgcgggaa tatttacgcc agcgtccccc cgcctgcata cggcctctta
     5701 atcaggtcga taacttattt attctgccgg tcgctgagtg tatttcgctc ggttgggata
     5761 gcagccgcca gacgctggat gcgcaggtaa tcagcggcga aggtgaagat aatctgctga
     5821 cgttatcact gccagcctca gccagcgcgc cttatgccgt tgaacgcatg gcggcgcttt
     5881 tgcaacaaac agacgacccc gtgtgtctgg tttctggctt tgtcagtttt gttgatgggc
     5941 aattgacact ggaaccacag gtaatgatga caaagactcg tgcctgggcg ctggacgcag
     6001 aaactgcgcc tgtggtggta tcactacctt ctgccagcgt tctgcctgtg ccgtctactg
     6061 ctcatcagtt gctgatgcgc tgccaggcgt tacttattca actgctccat aacggctggc
     6121 gctatcagga acagagcgct attagtcagg cagagttgct ggcgaatgac ctcaccgcgg
     6181 tgggttttta tcggctggca catgtgttgg cacaatttcg taatacagaa agcgaggcac
     6241 gggtagaagc aatgaataac ggtgttttgc tttgcgaaca attattcccc atgcttcagc
     6301 aacaaggatg aaatagtgct ttttactaag agttctactc cagttccgga ctgctcacgc
     6361 cacggtatta ggcatatcct atatagcccc tggtgagagt caccagtttc ttgattaaat
     6421 aaaatggagt tttacatgaa ggctttcaat aagctgtttt ccctcgttgt tgcatctgtt
     6481 ctggttttct ctcttgctgg ctgcggtgac aaagaagaat cgaagaaatt cagcgccaat
     6541 ctgaacggca ctgaaattgc cattacctat gtctacaaag gtgacaaggt gcttaagcaa
     6601 tcttctgaaa ccaaaattca atttgcctcc attggtgcaa ccaccaaaga agatgctgcc
     6661 aagacacttg agccgttaag cgccaaatac aaaaacatcg cgggtgttga agaaaaatca
     6721 acctataccg atacctacgc gcaggaaaac gtgactatcg atatggaaaa agtggatttt
     6781 aaagccctgc agggtatttc atgaatcaac gtttctgctg aagatgccaa aaaaggtatc
     6841 actatggcgc aaatggaact ggtgatgaaa gccgctggtt ttaaaga
//
LOCUS       DADLSB010000188         6884 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.188, whole genome shotgun sequence.
ACCESSION   DADLSB010000188 DADLSB010000000
VERSION     DADLSB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.188"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(102..1316)
                     /gene="rspA"
                     /locus_tag="JN331_003648"
     CDS             complement(102..1316)
                     /gene="rspA"
                     /locus_tag="JN331_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY7261666.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1521..1847)
                     /locus_tag="JN331_003649"
     CDS             complement(1521..1847)
                     /locus_tag="JN331_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HAY7261667.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1982..2323
                     /locus_tag="JN331_003650"
     CDS             1982..2323
                     /locus_tag="JN331_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY7261668.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2389..2918
                     /gene="speG"
                     /locus_tag="JN331_003651"
                     /pseudo
     CDS             2389..2918
                     /gene="speG"
                     /locus_tag="JN331_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2921..3631)
                     /locus_tag="JN331_003652"
     CDS             complement(2921..3631)
                     /locus_tag="JN331_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY7261669.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3739..4044
                     /locus_tag="JN331_003653"
     CDS             3739..4044
                     /locus_tag="JN331_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY7261670.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4243..5700
                     /locus_tag="JN331_003654"
     CDS             4243..5700
                     /locus_tag="JN331_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7261671.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWLETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTTKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     LRDGVRGKDKLDVPIKFIWNFKDLKRTLSRPEVLH"
     gene            complement(6371..>6884)
                     /locus_tag="JN331_003655"
     CDS             complement(6371..>6884)
                     /locus_tag="JN331_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261672.1"
                     /translation="VDVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTEPSFGSE
                     VSDYGIIAHESQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTA
                     LSFGCFRHDGENITVVLSRDGDSQSHDLEILGKVFVVAEAATTVKMGTGSATAVLPVV
                     KRLKFTKTEA"
BASE COUNT         1829 a         1763 c         1622 g         1670 t
ORIGIN      
        1 cttcgggata acgcttaccg ctcatgggca cctctcttta agccatctta aatgactctg
       61 aggtgtctgt taaacccgtg gcgattcaat tatcttactc cttacctgtt ccacagcgtg
      121 ccatcttcca gacgtgcgac tggtagataa gcaggttcat agggatattt cgccgccagc
      181 ttttcatcga attcgatacc aagacccggt ttgtctcccg gatgcatata gccgttatcg
      241 aaagtccagt tgtgcgggaa gacttcgagc atttgttcgg aataacccat gtattcctgg
      301 acaccgaaat tggggaccca cagatcaaag tgcagcgccg cagccatgca gactggtgac
      361 aaatcggaag gaccgtgtga gccagtacgt acctgataca gcgaagcaaa atcggcaatc
      421 cggcgcatac cggtaattcc gcctgcatgg gtcagcgtgg tgcggatata atcgatgagt
      481 tgctcttcaa tcagttgttt gcagtcccag atgctgttga agacttcacc cactgcgatg
      541 ggtgtgacgg tatgttggcg aatgagacgg aagcattcct ggttttccgc tggcgtcggg
      601 tcttccatcc agaacatgcg ataatcttca atgcttttac caaagcgcgc cgcttcaata
      661 ggcgttaagc gatggtgcat gtcatgcagc aaatgttcat taaaaccaaa cttgttacgt
      721 accgcgtcaa acaatttcgg catgaaatcg aggtatttct ccgtcgacca cagctgctct
      781 tccggccact gtcctttggt tgcgggttca taagccagac ctttaccttt cgacatgccg
      841 taggtggttt tcataccagg gattccgcac tgcacgcgga tggctttgaa tcccagctcc
      901 tgatgacggg cataatcatc cagagcttca tcaatactgt gaccggtggt atggcaataa
      961 accatcaccc cttcacgaga cgcgccgccg agtaactggt aaagcggcat gttggcagct
     1021 ttggctttaa tatcccacag cgccatatca accgctgaaa tggccgacat cgtaaccgga
     1081 ccgcgacgcc agtaagcacc tttatagaaa aactgccaga tatcttcgat acggtgcgca
     1141 tcgcgaccaa taagccgcgg acaaagatga tcctgcaaat aagaggccac ggaaagctca
     1201 cgtccattga gggtggcatc cccaaggccc gtaataccgt cctcagtggt gatttttaat
     1261 gtgacgaaat tacgccccgg acaggtaaca aaaacttcag cctttacgat cttcatgttc
     1321 gattccttgc atcgcttgtc gtgatgcatg aaatctacgc aactgagcca ctgccataca
     1381 agtatgaaga tcgaaaaagc cggagtgatc acaaaaaaag gcgtatattt gcgctgtgaa
     1441 tggttgacaa aagatgaaat agaatacctt ttgtcagctg acacttcctc gtatcttatt
     1501 gataaaatgg atttatgttc ctacgtgcgc ccccagcccg caacaatgat caacattccg
     1561 caaagcgcaa tcaacgcacc cgtccagtca taaagactta gtttcacgcc atccacaacg
     1621 cgcagccaca tcaatgccgt gcagacataa acgccaccat aagccgcgta aacacgccca
     1681 ctcgccgcag gatgcaacgt taacaaccag acaaacagcg ccagtgaaat ccccgccgga
     1741 agcaacagcc agatactggc gtttcgtttt aaccacaacc agggcagaaa gcatccaata
     1801 atttcacaca gcgcagtagc aaaaaatagt aacgttgttt taatcatctt tgtcacttat
     1861 tgacatcatg tatagttata gggcgacata atatcatcaa tataaacatc ctcctggtac
     1921 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
     1981 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     2041 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     2101 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     2161 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     2221 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     2281 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     2341 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     2401 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     2461 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     2521 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     2581 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     2641 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     2701 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     2761 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     2821 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     2881 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     2941 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     3001 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     3061 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     3121 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     3181 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     3241 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     3301 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     3361 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     3421 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     3481 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     3541 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     3601 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     3661 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     3721 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     3781 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     3841 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     3901 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     3961 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     4021 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     4081 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     4141 taataattct taacccaaaa tgggtaggct ccctctattg ttagcgcgct aaatattcaa
     4201 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     4261 atggtgggca tcagccgcag aacactcgtt aaatcaaccg caataggttc tctggcgctg
     4321 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     4381 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     4441 cgtctacatg ttaaagataa tgaagtgacc tggttggaaa ctgacaatac cggcagcgat
     4501 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     4561 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg ggaaacgtgg tgaaggcaaa
     4621 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     4681 gtcgaacaat atggcaatga agctgtatat attcagtact cttcggggat tgttggcggc
     4741 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     4801 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     4861 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     4921 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     4981 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     5041 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     5101 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     5161 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     5221 tataaagcct atattcttgg tgaaggtgac gataaaacag cgaaaacgcc gcagtgggct
     5281 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     5341 acaaaacccg cttatatctg ccagggctgg gggccgcaac gccaggcaaa cggcgagctg
     5401 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     5461 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     5521 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     5581 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     5641 ttcatctgga acttcaagga cttgaagcgg acccttagta ggcctgaggt cctgcattga
     5701 gtcaagacct tgtgtcattc tttgtgccag acttgtgtca ttagacggga tgtctagacg
     5761 gctggtcagg acccccggtg gcctcggtct ggacttgagg tgggcctatg gggggaagtc
     5821 tgctggctta atgtattaga tggtcattca catttttctg tcagaaacca tcggtcccac
     5881 tgtgagtcca aacctaagga cctagaagtc cacccacagc cttagacaga gtgtcgaaca
     5941 catccacacc gagtcgacca gagaggacca tgtagagacc cacagagagg aacacgagtt
     6001 tatagaccag agtcaggagg gacttcaggt ctgcggaagt catagacata aagcccctat
     6061 ctattatgtt atattattac attaacaaca gatccagacc attggtccag accatagtta
     6121 gttaatcaca gtgatagccc accacctgaa gtcctgctga tattcagtcc agacttacct
     6181 ctgctcatgg tcttacctcc ctcggtctcc tgaccttggg tatcgactac agcttgctga
     6241 gcgtcatctg gaggagctac aggaggagct acaggaggcg ggaccgaagc cccaccttga
     6301 gtcccaccac cacctgtcag gcccttggcg atggacctga agcctttact aatgctctta
     6361 cccattggtc ttacgcttcg gtcttggtga acttcaggcg tttgactaca ggcaggacgg
     6421 cagtagctga gccagtgccc atcttgacgg tcgtagcagc ttcagcgaca acgaacactt
     6481 taccgaggat ttccaagtcg tgggactgag agtcgccatc acgtgaaagg acgaccgtaa
     6541 tattctcacc gtcgtgacgg aagcagccga agctcagggc cgtgaagcgt ttagccaaca
     6601 tgaacacacc tttgtcctca ccgttgtaat acgcgatgtc ggtacccagc atatccacga
     6661 tagcgtcttc accagagaag ccctgtttag cagccagacg ggacagtgct ttcagttggc
     6721 tctcatgggc gataataccg tagtcagaca cctcagaacc gaaagaaggc tcggtgttct
     6781 catacagagc gtccatgata gcgtctgcct tagcggtcag gctaccagtc aggacaggaa
     6841 gctctacggt cttcagcagc ttagaggatt ccagaacgtc gacc
//
LOCUS       DADLSB010000189         6817 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.189, whole genome shotgun sequence.
ACCESSION   DADLSB010000189 DADLSB010000000
VERSION     DADLSB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.189"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(210..2063)
                     /locus_tag="JN331_003656"
     CDS             complement(210..2063)
                     /locus_tag="JN331_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY7261673.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADAGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATYAQQPALFTAMLKQFRADLTVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRMATAILNAAGRTSAFISGKA
                     PEIKPSPGGDTPSGPPEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSEGVFKITKQS
                     NKTWSLMHPVDDAITLLTQGGRLTCKFRLSGALTNNQFGLGIYLYTDAPVPDDVAMTG
                     TGNPFLMSYFTQTTDGRVNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGSATVTPK
                     LNGLAGPAFQVIKDSLTLGLNALTLTDVTKNAAYGVEIESLVLEINAPASS"
     gene            complement(2552..2628)
                     /locus_tag="JN331_003657"
     tRNA            complement(2552..2628)
                     /locus_tag="JN331_003657"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2592..2594),aa:Arg,seq:tct)
     gene            complement(2642..2718)
                     /locus_tag="JN331_003658"
     tRNA            complement(2642..2718)
                     /locus_tag="JN331_003658"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2682..2684),aa:Arg,seq:tcg)
     gene            complement(2728..2803)
                     /locus_tag="JN331_003659"
     tRNA            complement(2728..2803)
                     /locus_tag="JN331_003659"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2768..2770),aa:Ile,seq:cat)
     gene            complement(2853..3902)
                     /locus_tag="JN331_003660"
     CDS             complement(2853..3902)
                     /locus_tag="JN331_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAY7261674.1"
                     /translation="MANTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDGRAALGITAKQIADATGKKNMVPHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYMELLSEYKNLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRAIGVELET
                     GRFEQTVREVQDLIV"
     gene            complement(4011..4251)
                     /locus_tag="JN331_003661"
                     /pseudo
     CDS             complement(4011..4251)
                     /locus_tag="JN331_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4467..4847)
                     /locus_tag="JN331_003662"
     CDS             complement(4467..4847)
                     /locus_tag="JN331_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HAY7261675.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(4865..5854)
                     /locus_tag="JN331_003663"
     CDS             complement(4865..5854)
                     /locus_tag="JN331_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7261676.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLEAMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(5862..6671)
                     /locus_tag="JN331_003664"
     CDS             complement(5862..6671)
                     /locus_tag="JN331_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7261677.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVGPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLMVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
BASE COUNT         1571 a         1989 c         1619 g         1638 t
ORIGIN      
        1 cgttcccacc gctgacgtgg tgtatgacac acctgttgtg attttttcca cctggtacat
       61 accccgtctc ccgttatccg gaagctgaca acaataaaaa agccaccagt taagtactga
      121 tggctctgat aactcatgca ggcgtctcag acgacccact gacactaccg gtgagtttaa
      181 cgataccttc catttgactg gctcactttt tatgatgatg ccggtgcatt tatctccagc
      241 accagacttt ctatctcaac gccatacgct gcattttttg taacatccgt cagcgtcagc
      301 gcattcagcc ccagtgtcag actgtctttt atgacctgga atgccgggcc agccagtcca
      361 ttcagtttcg gagtaaccgt ggcactgccg gcggtgaaca ccagctccag cgtctgccag
      421 tcgttaccgt aatcgccgaa ctcccccagc ttcgtgtttc cggctttcct gtgatgcatc
      481 agattcactc tgccgtcagt ggtctgagtg aagtaagaca tcaggaacgg attaccagta
      541 cccgtcatcg ccacatcatc aggaacggga gcgtccgtat acagataaat ccccagcccg
      601 aactgattgt tggtcagtgc gcctgagagg cggaacttac aggtcagtct gccgccctgt
      661 gtcagcaggg taattgcgtc atccaccgga tgcatcaggg accaggtttt attgctctgc
      721 ttggtgatct taaatacacc ctctgacaac tgaattccgc cattcttaat gctccagccc
      781 tgcgcagcag catctccggc tgtcggcaac agggagattg tgcgtacgga tgcatcttca
      841 ggcggccccg atggcgtgtc gccgccgggc gagggtttga tttccggtgc cttaccactg
      901 atgaaggctg aggtgcgccc ggctgcgttc agaatagcgg ttgccatacg atccggaata
      961 atgctcctgc gcgcccatga actgaaatgt gtcgggcggt ttgatgatac ctggtttcca
     1021 ttcgttctcg atgccgcacc gtaatatcct gatgccggaa tatccggatc ttctgccggc
     1081 gcgttagtgg cggtattgac gccgttaccg tctgtcatga agggcacaaa ataaacgccc
     1141 tcactctccc tgtttttata cccgccgtac acggtgtcgt actgggtagc gtatgtattt
     1201 ttccagtaat acgtcgtgtc accacaaatc cacggcacat ctgcagcact gccaccatga
     1261 cactgcgcgt taaacacagt gaggtcagca cgaaactgct tcagcatggc tgtaaacagc
     1321 gcaggttgct gtgcgtaggt ggcggcgctc atgtcaaact ctccctgcat ccagcacacc
     1381 gccagcaaca catttttcgg gttcttctgt aatgcagctt tagtgcgcgc aatcaggtcc
     1441 tgatataacg gtttacccac cccccagcgc gccgaatcct ggctggcccc cgcgtccgca
     1501 ctgaatgtcc cctccgcgcc ctgggtgaat gccgaaccac cacgacagca tggtaccagc
     1561 aggatccccg cgttattcgg gatatacggg agcagttttt tggcaatatg taagccctgg
     1621 ccgacacagc cgtactgccc tttgctcagg tctgccttcg gatgattcag cgtactcata
     1681 tcctgcacat catgcagaca gtggtcagcc gggatgatgt cgttatacgt acaactctct
     1741 ccacccggag ttaccgtgct gcggcgcgcc agctgtttaa tgcgcggatc cggagcatcg
     1801 taagaatccg gtaacggaag cccttcaccg taggccatgc cgttggactg tccggcaagc
     1861 acaaccacgt agaaccagtc cggctcagat gaagggccga cctgtggatc tccttcaata
     1921 gccaccgcct gcatcagtgt gtacggcgta atggcaaccg gtccgccata tggctgccag
     1981 ccctctttca gtttgtgtgt cagcttttcc gcaaggtctg acggcgacgc cgccctgaca
     2041 acatcatagt gtttaaatgc catgaatcct cccggccggg ataatattgt gagtaaaatg
     2101 aggagcgggc tgaagtccgg aagttacagg acaatggcag aagagagaca acagcccgca
     2161 atacgaaaaa ggccgcgcta ttgcgcagag tgattactgt cggatattat tcgccagctg
     2221 aaatattact tcacgttttg ttgtttattc cttgccgccc gcgtctccca gcgcgggctt
     2281 tttttgtcca taagaaagcc cctccggaga ggggctggag agtggcgcta tgtgccattg
     2341 catggtgccg ggtgcctccc ggtgaattca gtaccagcac ctgaatccgc gattatccca
     2401 tatacctact cgctgattgc ccctccgcac agggggattc accatgccag tttcttttaa
     2461 caaactcccc gcaaaacaga caactgtcaa ccgtctgaat tgtgagacat ttaaaaaaag
     2521 gcccgcaaaa gcgagccggg aaaaataagt ctggcgcgtt gtactggatt cgaaccagtg
     2581 accgattgct tagaaggcaa ttgctctgtc cggctgagct aacaacgcag ggtacagata
     2641 atggaccgcc atcgaggact cgaaccccgc gcaaccagct tcgaaggctg gcgctctatc
     2701 ccgatgagct aatggcggta tgtgatatgg tggcccttgc tggatttgaa ccagcgacct
     2761 ggcgattatg agtcgctcgc tctcaccact gagctaaagg gccgcgcgca gaataataac
     2821 gttacggaat taatactgca atctcatccg tttcaaacga ttaaatcctg aacttccctg
     2881 actgtctgct caaaacgtcc ggtctccagc tcaacaccaa tcgcacgacg ccccagtgcc
     2941 atcgccgctt ttaccgttga acccgacccc atgaaaaaat ccgcaaccag atcaccagga
     3001 cgactacttg cgctgattat ctgctgcagc atttctgccg gtttttcgca cggatgtttc
     3061 cctggatagt actgcaccgg tttatacgtc cacacatcgg tgtacggcac ctgcaccgtc
     3121 acaccgaaat accgccgcaa atttttatat tcactcagca gttccatata ctgccggttc
     3181 agctcactgt atgtgctgac cagctggtgg tgtggctttt ccagttcccc gcgctgatgt
     3241 ttctcttctg ccacccgggc aaacagcgac tgtaatttca gataatcgct ttcgttcggt
     3301 agctgccact gactggcact gaaccagtgc ggcaccatgt ttttctttcc tgtggcatct
     3361 gcaatctgtt ttgccgttat ccccagggca gcgcgcccat cacgaaagta agcaatcagc
     3421 ggggccatca catgctgttt cagtgccctg cccttcgcct catacccggc atctttcgga
     3481 cgatacggcc cctgataatg ttccgcgaac agaatgcgct ctgtggcggg gaaatacgcc
     3541 cgcaggcttt ccttgttgca tccgttccag cgtccggacg gcttcgccca gataatatgg
     3601 ttcagcacac tgaagcgttc acgcatcatg atttcgatat cagatgccag gcgatgacca
     3661 cagaacaggt aaagacttcc ggcaggtttc agcacccgcc agaactgcgc cagacactgg
     3721 tccagccact tcaggtaatc atcgtcgccc ttccactggt tatcccagcc ctcaggcttc
     3781 actttaaagt acggcgggtc cgtgactatc aggtcaacag aattttcggg taacgaccgg
     3841 ataaattcca ggcagtcggc gttgattaac tcacaactgg atatttttac agtattagcc
     3901 atagatcaat aagcacttct ctgataggct cataccgctt ttgcgcaaag cagatgggcc
     3961 tgaggtttgc ttgtgacccc aacgcatgag cagatggctg gtgggtgccc ctaacaccca
     4021 ccagccgccc atttaccaca aataaaaaag ccttcactgc ggaaggcgtc tgtaacaacc
     4081 gaactgatag tctgccagac ccgccataac cagctgggtc agtattaact ggcagcgttc
     4141 gcgtgaaagg taagtattct gcgcaatctc cccgactgtc gccggttcgg tgacgcttaa
     4201 ttcattaaac accactctgg cggtttctgt catatcctgc tgtttcagca tgtctttttc
     4261 cctttttcgg ttaacgtgac acaccaataa ctcttgtcga aaaagccagc aagctgaaag
     4321 acaggtattc accgccacca gcacgtttac tgtactggac cgatttcagc cataaaaaaa
     4381 cccgctcgcg gcgggtttaa gctgtgtggc gaagtaacca ctcttaacat actgacatac
     4441 tttttgcgga ccgcactaat cattttttac ttttttagca gccagtcgtc catctccagt
     4501 cttaccccca gcacagacaa acatccgtca ataaaccctt cggctatctg catctcaatt
     4561 cgtattgctt tttcgctttt ctttctcgtc ctggctatct gtctttttga tattcgcaac
     4621 aaataatgag caatgagaag cgaatactcc tcaggttttt tctgcttcag acgagcaaga
     4681 cagttttcaa tgataagtcc gtcatcatcg cagcaggccg gacgtggttt agtggcagat
     4741 ggtaaaagtc ctttgaatcc ggcagcgatc ggagaatagt ccaccccggt gttaccactt
     4801 gcagcccatg ccccccagcg ttcaagaacc atctgaatat cacgcatcaa ctttctccac
     4861 aaaatcagga cagcacacca atcgccagcg cgcgatcgat aaaacgaaat atcagctcca
     4921 gttgggaacc atacttctct tcaaatgcca cggtatccgc atgcagttcg tcatggtgtt
     4981 ttctgcacaa aggcaacaca aaaaggtcat gcgcttttgt acccattcca ccctgaccat
     5041 gaccaatcag gtgatgagga tcgtcggctg gcttaccaca acatgcacac ggctgcgtct
     5101 taacccagcg cgtgtacttt tcattaaccc agcggcgacg ttttgggcgt aacataaaag
     5161 actccggcga ctccggatcc actttcagcg ccagcacctt tttcgcctta tcctggatga
     5221 tgctggtggc aggaaccgaa ggcacaaggt cactttcccg ggtgacagac ggcacaacag
     5281 gcttcggtaa tctcagtgcc ttacgggctg cactttccgg taaggcatcc gccaggtcat
     5341 tacgaatcag ccaccagcac agttccggca ttgtcacaac gtgactgtca tcaaaaccaa
     5401 gatcacggcg cacaacagac aacacccagc gggcacagtt atccgttgcc attgcttcca
     5461 gccgttccgt gaactggtca cggagaagat tgtcacagtg ccagcacaga cggattgcgc
     5521 ctggcgcgtg ccgcattgtg gtcatgttct cgctgtgcca gtcggaatga ggccactgac
     5581 agcccttttc acgaagtaac cagctctcaa ggcattccac gccaccagca cgacggatca
     5641 ccgcctcatg gcggaacacg gcccgaacgg caggatcatc cgccagcggt tgtgatgctg
     5701 ccggaacggc accgctggcg aaagatgaat aacgttccgg ctcaggctcc agcaggacac
     5761 gcccctgcat aaacaggggc atcagctctg aacctggcct gaacaatacg atccccatac
     5821 gcggggcaat ttcaggggtc agtagtgctc tcacggtcac ctcaatgaac ggtatcgagc
     5881 agctttaaca gctcagggaa tcgggattcg aagaaatgcg gctgcgtctc gcgcggattt
     5941 gcgggactgg tgatgttctt gccgaacatg caacctttcg ctgtcagcga ccagaatttt
     6001 ttgatgttgt taatcgcggt acggctgtat cgttcgcgtt gttcaacgat ccccagcttc
     6061 gccatctggt gatatgcctg attagccgtc aggcggatac catactgctt cagcagtgca
     6121 ctcagcgaca gcgtcgggcg gcttgagcca tcaggcgcgt cagcaggtgc atcaatggca
     6181 tagcgcggtg ccagattcgg taagccaaca gcctcctgga gtttctgaca ggccccaagc
     6241 acagatgagt tagacagatt taactcccgg cgcataaagt ccagcagaat cacgccagcc
     6301 tgcatcttgt cagcagcctg tccggataac ttttccggcg cgctggttac catatcgaaa
     6361 gtacggatca ccatcagatg gaatgacggg ctgatccaca ttgcataggc atacaccagt
     6421 tccttacaga catacgttcc ccgttcattt cccccatgaa tcacactcac cgggccaaca
     6481 cccaaattct gggtgttggt taattcatga acaagctcaa cagtttgttg gctggaaaga
     6541 aactttcctg gctccttggt tctggcattt gcaccagatg ctactgctgc gcgatgcaga
     6601 tcgttcaggc tgtaacgccc ataagcatca cgacgaactt caataccatc aatgaccatc
     6661 agattattca tacttcgttt ctcctcttaa tcaggcagct gcacccgccg ttttctcgta
     6721 cttactgata gtgatctcga ccttcccttc cgggataacc ggtccccact ccaccagcat
     6781 tcttttcacc tgactgtcgt cttcccaccc ccccgcg
//
LOCUS       DADLSB010000190         6810 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.190, whole genome shotgun sequence.
ACCESSION   DADLSB010000190 DADLSB010000000
VERSION     DADLSB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.190"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(228..303)
                     /locus_tag="JN331_003665"
     tRNA            complement(228..303)
                     /locus_tag="JN331_003665"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Phe,seq:gaa)
     gene            complement(409..1116)
                     /locus_tag="JN331_003666"
     CDS             complement(409..1116)
                     /locus_tag="JN331_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY7261678.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLDALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1514..3649
                     /locus_tag="JN331_003667"
     CDS             1514..3649
                     /locus_tag="JN331_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY7261679.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACSGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFVPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3698..4954)
                     /locus_tag="JN331_003668"
     CDS             complement(3698..4954)
                     /locus_tag="JN331_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY7261680.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5156..6235)
                     /gene="mltC"
                     /locus_tag="JN331_003669"
     CDS             complement(5156..6235)
                     /gene="mltC"
                     /locus_tag="JN331_003669"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY7261681.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6300..6575)
                     /locus_tag="JN331_003670"
     CDS             complement(6300..6575)
                     /locus_tag="JN331_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY7261682.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6603..>6810)
                     /locus_tag="JN331_003671"
     CDS             complement(6603..>6810)
                     /locus_tag="JN331_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7261683.1"
                     /translation="NLTQLTAFRHTFSHFHLDIVPMWLPVSSFTSCMDEGNALWYNLA
                     QPPSVGLAAPVERLLQQLRTGAPV"
BASE COUNT         1666 a         1658 c         1784 g         1702 t
ORIGIN      
        1 ccgtgcagta atgaatccgt tagccaggtg ctcatgtgga aatagtgtct attggggatt
       61 tgaagtttga cagaaaaggc cacagtgacc agaagggggc tacttagaag aaatgttcag
      121 gctggatgga agaggtaaag cagtgacatc agcattcaaa gtgaccggat atctaccaga
      181 accccagaaa gcaaaaaacc agcccgtagg ctggttcttc taaatagtgg tgcccggact
      241 cggaatcgaa ccaaggacac ggggattttc aatcccctgc tctaccgact gagctatccg
      301 ggcaacgggg cgcattaaac ctgattcgcc tcgtctcgtc aatcaaattt cttcaattta
      361 ctgcagactg cacaatctat catcactttg ccgctattgc acgcattgtc aggcaaacca
      421 ggcggtccag gcggcggaaa gcggcattgc cagaaggagt gctggaagca tgttgaccac
      481 cgggaacatt ttaatgccac agatgcgtaa tccggttgcc agcagcaata aaccgcctac
      541 ggcgctgaag tctgccatca tcgacggtgt ggtcagcggt aatatcagcg cggcagccca
      601 cgccagcgtt aactggatga tcagtaatgg gatactaatt accgacaccg caatacccag
      661 cgagcaggcg aagatcatcg ccgtaaagaa atcaagaaat gacttggcga ttaaaatact
      721 cggatcgccg gtcatccctt cgttcatcgc cccgaagatc ccggtgccgc tggcgcaaaa
      781 caggacaata atcgcgacat aattctgaat aaaagattca tgcgctggct tcttacgtga
      841 gtgacgaaac agattttgtg ctttggcgac tgctgtattg acacctttct ccagcaaaca
      901 aatttcgcca attagagcgt caagtagcgt agctaaaacc atcgctggaa ggttggcgca
      961 tttcacaacc agtaaaatac caatccccag cgatgccaga ccaaaaatag atgtcatgga
     1021 gatgcggata cgttccggta agcgttggct gagcagtgcg ccgagaacgc cacccagtaa
     1081 gactgcacta gcgttgataa aagggccgat gaccacaaga gttcctgtta gctgtattgt
     1141 ttgattttct tattatgaac tttttgcctt gttgatggct tgtctggtcg caaagtcctg
     1201 gcttgcacgc cttagcgaaa ggtgccatga ttgcgcgaat tttctcctct ctgtacggag
     1261 tttgcccgat gcacgccatc tccttacatt ctctcgctta tcgccgtttc gcgcgaaacg
     1321 tttccctttc tatgttactg ctcatgcggt gaagttaacg cacgctcact gcaggacaac
     1381 agtaaaatca gagcgtttct gcttttactg atgtctggcg gtcggagctg gtgaccagtt
     1441 tgacccatat ctcatggggc agggttttcc accttgtccg gtattcttac ttccccgaaa
     1501 cgggtttgcg cttatgaaat caatgaatat tgccgccagt agtgaactgg tatcccgact
     1561 ttcttctcat cgccgcgtgg tggcgttggg agatactgat tttacggacg tcgcggcagt
     1621 cgtcattacc gctgcggata gtcgcagtgg cattcttgcg ttgcttaagc gcaccggttt
     1681 tcatctaccg gtgtttttgt attccgaaca tgctgttgaa ttacctgcgg gcgttacggc
     1741 ggtaatcaac ggcaacgagc agcagtggct ggagctggaa tccgcagcct gtcagtatga
     1801 agagaatttg ctgccaccgt tttatgacac gctgacgcag tacgttgaga tgggcaacag
     1861 cacctttgct tgctctggac atcaacatgg tgcgttcttt aaaaagcatc ctgccggacg
     1921 ccatttttac gatttctttg gtgagaacgt ctttcgcgcc gatatgtgta acgctgacgt
     1981 aaaattgggc gatctgctta ttcatgaagg atcggcgaaa gatgcgcaga aattcgcagc
     2041 caaagtcttt catgccgata aaacctattt tgtgctgaac ggcacatcgg cagcgaataa
     2101 agtggtgacg aatgcgctgt taacgcgtgg cgatctggtg ctcttcgacc gtaacaacca
     2161 taagtcgaat catcacggcg cgctgattca ggcggggacg acgccggtct atctggaagc
     2221 ttcacgtaac ccgtttggtt tcattggcgg tattgatgcg cactgtttta atgaagagta
     2281 tctgcgccag caaattcgcg acgttgcgcc agaaaaagcc gacctgccgc gcccgtttcg
     2341 cctgacgatt attcagctgg gaacctatga cggcactgtc tataacgccc gtcaggtgat
     2401 cgataccatt gggcatctgt gtgattacat tctgtttgat tccgcgtggg tcggttacga
     2461 acagtttatc ccgatgatgg cggatagctc gccgctgctg ttagaactta acgaaaacga
     2521 tccggggatt tttgtgaccc agttggtgca caaacagcag gctggattct cacagacgtc
     2581 gcagatccat aaaaaagata accatatccg cggacaggcg cgtttttgcc cgcataagcg
     2641 gttgaataac gcctttatgc tccatgcttc taccagccct ttctatccgc tgtttgctgc
     2701 actggatgtt aacgccaaaa ttcatgaagg ggagagtggg cgtcggctgt gggctgagtg
     2761 cgttgagttg ggtattgagt cgcgcaaggc tattcttgcg cgctgtaagc tgttccgccc
     2821 gtttatcccg cccgttgttg atggcaaatt gtggcaggat tatccgacat cagtgttagc
     2881 cagcgaccgc cgttttttca gttttgtgcc gggggcgaag tggcacggct ttgaaggata
     2941 tgccgcggat cagtattttg ttgatccgtg caagctgtta ctcactacac caggtatcaa
     3001 tgccgaaacc ggcgaatata gcgactttgg cgttccggcg acgattctgg cgcactatct
     3061 gcgtgagaac ggcattgtgc cggagaagtg cgatctcaac tccattctgt tcttattaac
     3121 tccggcggaa agccacgaga agctggcaca actggtggcg atgctggcgc aatttgaaca
     3181 gcatattgag gatgactcgc cgctggctga ggtgttgccg agcgtttata acaaatatcc
     3241 ggtgcgctat cgcgactaca ccctgcgcca gttgtgtcag gagatgcacg atctgtatgt
     3301 cagtttcgac gtcaaagacc tacaaaaagc gatgttccgc cagcagagtt tcccgtcagt
     3361 ggtgatgaac ccccaggatg cgcatagcgc ttatattcgc ggtgaagtgg agttggtgcg
     3421 gattcgtgat gccgaagggc gaattgcggc agaaggggcg ttgccttatc cacctggcgt
     3481 gctttgcgtg gtacccgggg aagtctgggg tggggcggtt caacgttatt tccttgcgct
     3541 ggaagaaggc gtgaatttgt tgccgggatt ttcgccggag ttgcaaggtg tctatagcga
     3601 aaccgatgcg gatggcatga aacgattgta cggttatgtg ttgaagtaag aataaaaaaa
     3661 cgggtcacct tctggcgacc cgtttttctt tgcgtaatta gtggctaacc gtctgtgtgc
     3721 ctgtcgggac acgaacgtgt ttatatttga acatcgccat gaacgcgaag gccagaacca
     3781 cggaataacc agcgaaaatc aaccatacgg tctgccagtc ggtaatgccg ttttgggtgt
     3841 acatctcaac aactttaccg ctcacgatgc cgccgaggat acagccgaag ccgttagtca
     3901 tcatcaggaa catcccttgt gcactggcgc gaattgccgg gctaacttct ttttcgacaa
     3961 acaccgaacc agagatgttg aagaagtcga atgcgcaacc gtaaacgatc atcgacagta
     4021 ccagcagtac agtaccgaac ggagtcgggt cgccgtaagc aaacagcgca aaacgcagga
     4081 tccacgccac aatactgatc atcattacgt tcttaatacc gtagcggctt aagaagaacg
     4141 ggatggtcag aatgaacagg gtttcagaga tctgcgaaat cgacatgatg attgacgcat
     4201 gctgcacgat gaagctgctg gcaaacatcg gatctttgtc gaagctgtgc aggaaggtat
     4261 taccgaacat gttggtaatc tgcagttccg cgcccagcag cattgagaag atgaagaaga
     4321 ttgccatacg cttgttttta aacagcgcga atgcatcgag gcccagcagg gttgtccagc
     4381 tctgattcgc ttgctgttta gcaaccggaa tatgcggcag agtcagggta aacagaacca
     4441 gaatggcgga aagtgctgcg ccaatataca gctgcatgtg gcttaattcg aagccagaca
     4501 ggctcaccac ccacattgcc atgataaagc cgatggtgcc ccagatacgg attggcggga
     4561 agtcagtaac gatatccatc ccggcatttt gcaggcgata gtaagagatg gtgttgatta
     4621 acccaagcgt tggcatataa gcaaacgagt taatcaatat cacaaggaac atcgcttccg
     4681 gcgtcgtgac ctgtgccgcc atgaacagcg tgatagcgcc aatggtgtgg caaatggcat
     4741 atacccattt cgcacttaac catttgtcgg ccacaatccc cagcagcgca ggcataaata
     4801 ccgcggcgat acccagtgag ctataaactg cgccaataga agcaccgtca aacttcaggg
     4861 taacaaacat ataggagccg agggtcgtca gccaacttcc ccacagacag aactgcagaa
     4921 aagagaggat tttcagctgc agcttaagat tcatgttaat ttcctcacat cgtgatgcgg
     4981 atgagtgttt gaaaaagcac atttgaccgt gcgagatgat gggattctat caacttcgcg
     5041 gactggtttt ttgttacctg tggcaaataa ttgcaaacat tcccaatttg caaaacgaaa
     5101 ttgtgatgtg gataacattt ttgcccctga gcatcgtcag gggcggttaa tggaattatc
     5161 ggcggcggta ggatttttgc gcggtattca ctttataaag ataacggcga gattccgcag
     5221 agggatggcg ggtcgtcagc gtctgataaa catcgcccgg cgtcatggtg ttaataatat
     5281 tggcagcctg aatcttatca ttcgaaaaga ctcgcagcac gctgcctgcg ccgccgttat
     5341 aggcggtgat gacggcataa cgccgcgatg ttgggttatc aattccgccg agataaacat
     5401 tgttcagcat cgccagatac gcggtgccgg tatcaatatt gctggcagga tcaaacaaga
     5461 aactgcggct cggcgtgccg gatttcccct gcgagcggaa cacatctttc ccggcagtat
     5521 tttgtaccac ctgcattaat cccagcgcat cggaacggct gaccgcatac gggttaaagg
     5581 aagattcagt ctgcataatt gccagaatca gcgactcatc aacgccatat ttccgtgacg
     5641 cctggcggac catgccgaga tatttgtgcg cacgtttatc aaggtggttc ggcaccatgt
     5701 taatggtgac gctgtagatg atacgtagcc cgttgctgcg gcttttcaga cggtttttca
     5761 gcagataatc cgcgaagttg cttgcgcgac cttcccagcg aatcggctgc ccggtgttgt
     5821 ccaccacctg accgtaaagg aaaggttctt tcgaaattgt aatatcatca acgtcggaat
     5881 agagatcgac cgaactcgga tcgtcaccca tcagcaacgt tttgataatt gcccggcgca
     5941 aatgcgcggc aggttctgtc ccggcgatgg tttcgatagt aatcgtaccg tcatcgaagt
     6001 tgatgtggct gcgggtctga tattgatcgg tgtatttcac gtagtcctta ggaccggcga
     6061 tcaccacctc tttgaagccc cagatgttct caatattgtg ggcaaattgc cccatcagaa
     6121 tatcaaaacc gttggtatct ttgacccagg cttcgttata ggtatcgcct tttttggtcg
     6181 tcgaacagga gatgagcaac ggcgcaatca aagccagcgc gagatatttt ttcatcattc
     6241 cgggtgcgtg ttgtgtttgt ttttatgcaa gctgccggag gcgtgctccg gcactgtttt
     6301 tattttttat cttccggcgt atagccctcg atatgcacct ctttaccctc gaacaggaag
     6361 ttgaccatct cctgctccag cagcttgcgg tgctcggcat tcatcatgtt gagtttcttt
     6421 tcattaatca gcatggtttg tttgtgctgc cactgcgccc aggcttcttt ggagatctcg
     6481 ttatagatgc gttttcccag ctcgccgggg tacagctgaa aatcctgacc ttctgcttca
     6541 cgttgcagga aagtacaaaa aatcgttctg ctcataaatc atcctcttta tcgactcacg
     6601 cgctaaaccg gcgcgccagt gcgtaactgc tgtaacaaac gctccacggg agccgccagg
     6661 ccaactgacg gcggttgcgc taagttatac cagagcgcat tgccttcatc catgcagctg
     6721 gtgaatgacg acacgggaag ccacataggc acaatatcta agtggaaatg gctgaaggta
     6781 tggcgaaacg cggtcagttg cgtcaggtta
//
LOCUS       DADLSB010000191         6769 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.191, whole genome shotgun sequence.
ACCESSION   DADLSB010000191 DADLSB010000000
VERSION     DADLSB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.191"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(101..739)
                     /gene="rutR"
                     /locus_tag="JN331_003672"
     CDS             complement(101..739)
                     /gene="rutR"
                     /locus_tag="JN331_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY7261684.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1027..2118
                     /gene="rutA"
                     /locus_tag="JN331_003673"
     CDS             1027..2118
                     /gene="rutA"
                     /locus_tag="JN331_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY7261685.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIIARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2118..2810
                     /gene="rutB"
                     /locus_tag="JN331_003674"
     CDS             2118..2810
                     /gene="rutB"
                     /locus_tag="JN331_003674"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY7261686.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2822..3208
                     /gene="rutC"
                     /locus_tag="JN331_003675"
     CDS             2822..3208
                     /gene="rutC"
                     /locus_tag="JN331_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY7261687.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            3216..4021
                     /gene="rutD"
                     /locus_tag="JN331_003676"
                     /pseudo
     CDS             3216..4021
                     /gene="rutD"
                     /locus_tag="JN331_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
     gene            4031..4621
                     /locus_tag="JN331_003677"
     CDS             4031..4621
                     /locus_tag="JN331_003677"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY7261688.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4632..5126
                     /gene="rutF"
                     /locus_tag="JN331_003678"
     CDS             4632..5126
                     /gene="rutF"
                     /locus_tag="JN331_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY7261689.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     SVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5147..6475
                     /gene="rutG"
                     /locus_tag="JN331_003679"
     CDS             5147..6475
                     /gene="rutG"
                     /locus_tag="JN331_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY7261690.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6557..6730)
                     /locus_tag="JN331_003680"
     CDS             complement(6557..6730)
                     /locus_tag="JN331_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY7261691.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
BASE COUNT         1436 a         1826 c         1859 g         1648 t
ORIGIN      
        1 ttcgctgagt gtgaataccg ctgccgctgg cggtaacgcc agtttaatga cgatcggtta
       61 aacaggtagc cggaggatgt tacaacctcc tccggcatct ttaacgtggt cgaatcccct
      121 caataataat ccgctgcacg ttttcaaccg tttgattgaa aaatacctca tcgcgcaacg
      181 tcgcgcctgt caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc
      241 aaatcataaa aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac
      301 cggcaatcag cgccgatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca
      361 ttaacaacgg cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat
      421 cgcgtgagac ttccagcttc agacggatgt actcttttat cgccgccagc ggggcgaaat
      481 cttcacgaaa cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa
      541 tatacagcgc ctcttttgac gggaagtaat acagcagatt ggtttttgaa acacccgcca
      601 actctgcgat ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg
      661 ctgcgctaag aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt
      721 tcactgcgcc ttgcgtcatg cgctctcccc tcttgtagat tggtaacagg atagcaaaac
      781 ccccgcccgg cctcgacctt ctgcactctc atcgcgcacg gatgcatgtt ttatgtgcaa
      841 ctgttttgac cgtttagtcc actttttacc agatatttta gataacacaa tgctaacaca
      901 ttaataacat tcacttttta aaaactggca tccgctttgc aaacaagcca atacgcggct
      961 gaaaaggata tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg
     1021 ttgatgatga aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc
     1081 cacgcgccgc agtacatgcc gacctttgaa ctgaataaag ccattgtgca aaaagcggag
     1141 cattaccatt tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact
     1201 gagttctggg atcacaacct tgagtcgttc accttgatgg cgggtctggc agccgtgacc
     1261 tcgcgcattc agatttacgc cactgctgcc accttaacgt tacctccggc aatcatcgcc
     1321 cgtatggccg caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact
     1381 ggctggcaaa agcccgagta tgagcagatg ggtatctggc ctggcgatga ctatttctcc
     1441 cgtcgttacg actatctcac cgaatatgtt caggtgctgc gcgacctgtg gggcacgggg
     1501 aaaagcgatt ttaaaggcga ttttttcacc atgaatgatt gtcgcgtcag tccgcaaccg
     1561 agtgtcccca tgaaagtgat ctgcgccggg caaagcgacg ctggcatggc gttctccgcc
     1621 cagtatgccg atttcaactt ctgtttcggc aaaggcgtaa atacacccac ggctttcgcc
     1681 ccgaccgctg cgcggatgaa acaggccgca gagcaaaccg gacgcgacgt tggctcttat
     1741 gtgttgttta tggtgattgc cgatgaaacc gacgatgcca ctcgcgccaa atgggaacac
     1801 tacaaagcgg gcgcggatga agaggcgtta agctggctaa ccgaacaaag tcagaaagat
     1861 acccgctccg gtactgacac caacgttcgt cagatggccg atcccacttc ggcggtaaac
     1921 atcaatatgg ggacgttagt cggttcttac gccagtgtcg cgcgcatgtt agatgaagtc
     1981 gcaagcgtgc ctggtgcaga aggcgtgctg ttgaccttcg atgattttct gtcgggaatc
     2041 gaaaccttcg gcgagcgcat tcaaccactg atgcagtgcc gcgcccatct ctctgcgctg
     2101 actcaggagg tggcatgatg acaaccttaa ccgctcgacc ggaagccatt accttcgatc
     2161 cgcagcaaag tgcgcagatc gtggtggata tgcaaaacgc ttatgccacg ccaggcggct
     2221 acttagatct cgccgggttt gatgtctcaa ccactcgccc ggtcattgcc aacattcaaa
     2281 ccgctgtgac cgcagcgcga gcggcaggga tgttgatcat ctggtttcaa aatggctggg
     2341 atgaacagta tgtcgaggct ggcggccccg gctcaccgaa ttttcataaa tcgaacgccc
     2401 tgaaaaccat gcgtaagcag ccgcagctgc aggggaaatt gctggcgaaa ggctcctggg
     2461 attatcaact ggtggatgaa ctggtgccgc agcctggcga tattgtgctg ccgaagccgc
     2521 gctacagcgg tttcttcaat acgccgctgg acagcatttt gcgcagccgt ggaatacgcc
     2581 atctggtttt caccggtatc gctaccaacg tctgcgtcga atcgacgcta cgcgatggct
     2641 tttttctgga gtatttcggc gtggtgctgg aagacgcaac acaccaggcg gggccggaat
     2701 ttgcacagaa agccgcgttg ttcaatatcg aaaccttttt tggctgggtc agcgacgtcg
     2761 aaacgttctg cgacgcgctt tctcccacgt cctttgctcg tatcgcttaa ggagtttaac
     2821 gatgccaaaa tccgtaatta ttcccgctgg cagcagcgca ccgctggccc ccttcgttcc
     2881 cggcacgctg gctgatggcg tggtgtatgt ctccggtacg ctggcttttg atcaacataa
     2941 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
     3001 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
     3061 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
     3121 taaaccggcg cgattctgca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
     3181 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
     3241 cttatgctga tgcacccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
     3301 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
     3361 gcaccggcaa taatcccgac acactggcag aagattacag tatcgcccag atggcagcgg
     3421 aactgcatca ggcgctggta gccgcaggga ttgagcgtta cgcggtggtc ggccatgcgc
     3481 tcggtgcgct ggtggggatg cagctggcgc tggattatcc cgcgtcggta actatgctgg
     3541 tcagcgttgc gttaacggct ggctacgaat aaacgcccat acgcgccgct gttttcaggt
     3601 tcgcgaacga ttactgtata gcggcggcgc gcaagcatgg gtggaagcgc agccgttgtt
     3661 cctctatccc gccgactgga tggcggcccg cgcacctcgc ctggaggcag aagacgcgct
     3721 ggcactggcg cattttcagg gcaaaaataa tttactacgt cgacttaacg ccctcaaacg
     3781 cgctgacttt agtcaccatg cggatcgcat ccgctgcccg gtgcaaatca tctgcgccag
     3841 tgatgatctg ctggtgccaa cagcatgttc cagtgaactt catgccgccc tgcccgatag
     3901 ccagaaaatg gtgatgccct atggcggaca cgcctgcaac gtgaccgatc ccgaaacgtt
     3961 taatgctctg ttactcaacg ggcttgccag cctgttacat caccgtgaag ccgccctgta
     4021 aggaattgct atgaacgaag ccgttagccc aggtgcgctt agcaccctgt tcaccgatgc
     4081 ccgcactcac aacggctggc gggagacacc cgtcagcgat gagacgttac aggagcttta
     4141 tgccctgatg aaatgggggc cgacatcagc taactgttct ccggcacgga tcgtgtttat
     4201 ccgcactgca gaaggaaaag aacgtctgcg cccggcgctt tccagcggca atctgcaaaa
     4261 aaccctgacc gcgcccgtca ccgctatcgt cgcctgggac agtgaatttt atgaacggtt
     4321 accactactg tttccccacg gtgatgcccg cagttggttt acctccagcc cacaacttgc
     4381 cgaagaaaca gcgtttcgca acagttccat gcaggcggcc tatctgatcg tcgcttgccg
     4441 tgcgctggga ctggataccg gcccgatgtc gggttttgac cgtcaacacg ttgacgacgc
     4501 cttttttgcg ggcagcacgc tgaagagcaa tctgctgatt aatatcggct atggcgatag
     4561 cagcaaactt tttgcgcgcc tgccacgtct gtcctttgaa gaagcctgcg ggctgttgta
     4621 aggagcaaac tatgaacata gttgatcaac aaacctttcg cgatgcgatg tcctgtatgg
     4681 gcgcggcggt caatatcatc accacggacg gtccagccgg gcgcgccggg ttcaccgcca
     4741 gcgccgtctg cagcgtgacc gacacgccgc ccacattact ggtgtgcctg aatcgtgggg
     4801 cgtccgtctg gccggtattc aatgaaaatc gaacgctgtg tgtaaataca ctgagcgccg
     4861 ggcaggagcc gctttcaaat ctttttggcg gcaaaacgcc catggaacac cgctttgccg
     4921 ccgcccgctg gcagacgagt gtgaccggat gcccgcaact ggaagaggct ctggtttcgt
     4981 ttgactgccg tatcagccag gtggtgagcg tcggcaccca cgacattctg ttttgcgcca
     5041 tcgaagcgat tcatcgtcac accacaccct acgggctggt gtggttcgat cgcagttatc
     5101 acgcgctgat gcgccctgct tgttaacctc cttaaggaga cagctgatgg caatgttcgg
     5161 ttttcctcac tggcagttaa aatcgacctc tacagagagc ggcgtggtcg cgccggatga
     5221 acgattgccg tttgctcaga cggcagtaat gggcgttcaa cacgcggtgg cgatgtttgg
     5281 cgcgacggtg ttaatgccga ttctgatggg gctggatccc aatctttcca ttttaatgtc
     5341 ggggatcggc acgttactgt ttttctttat taccggcggg cgcgtcccca gttatctcgg
     5401 ctccagcgcg gcttttgtcg gcgtggtgat tgccgcgacg ggctttaacg gtcagggcat
     5461 taacccgaat atcagcatcg cgctgggcgg gattatcgcc tgtgggctgg tttataccgt
     5521 cattggcctg gtggtgatga aaattggtac acgctggatt gaacgactaa tgccgccggt
     5581 ggtgacgggt gcggtggtga tggcgattgg cctgaactta gcgcctatcg cagtgaaaag
     5641 tgtatcggct tcggcctttg atagctggat ggcggtaatg acggtgctgt gtattgggct
     5701 ggtggcggtg tttacccgtg gcatgatcca gcggttattg attctcgtag gattaattgt
     5761 cgcctgtctg ctgtatggcg tgatgaccaa cgttttaggg ctgggaaaag ccgtcgattt
     5821 tactctcgtc agctatgctg cctggttcgg tctgccgcat ttttccactc ctggttttaa
     5881 tggccaggcg atgatgttga ttgcgcccgt ggcggtgatt ctggtggcgg aaaacttagg
     5941 tcatctcaag gcggtcgccg ggatgaccgg acgcaatatg gacccgtata tggggcgagc
     6001 attcgtcggt gatggtctgg caacgatgct ttccggctct gtcggcggta gcggcgtcac
     6061 cacctatgct gaaaatatcg gtgtgatggc cgtgacaaaa gtctactcga cgctggtgtt
     6121 tgttgcagct gccgtcatcg ctatgctgtt gggcttttcg ccgaaatttg gcgcgctgat
     6181 ccataccatt ccggccgcgg tgattggtgg tgcatcaatt gttgtgttcg ggctgattgc
     6241 cgtcgcaggc gcaaggatat gggtacaaaa ccgtgtcgat ttaagccaga acggtaattt
     6301 gattatggtc gcagtaacct tcgtgctggg cgcgggtgat tttgctctca cgcttggcgg
     6361 ttttacgttg ggagggatcg gtacagcaac ctttggcgcg attttactca atgcgttatt
     6421 aagcagaagg ttggttgacg ttccaccgcc agaagttgtt catcaggagc cataaacaga
     6481 aaaccgccgg gttaccggcg gtgatgactt tttgacgagc ttaagaaaaa aatgtattcg
     6541 tcattgatta gcccggctag ttgtcgcttt tgccgtgact gctcttgcca ccttttttac
     6601 ctgcttcaga tgcgcgctgc ggatcattct tgaaattacc cccgctgtgc tgtccacctt
     6661 ttttacctgc ttctgatgct ctttcgcggt cttctgcaaa attgccggaa ccgcctcgat
     6721 ggtttgccat tactgacctc cgtctgggta tagacatcat attgcatag
//
LOCUS       DADLSB010000192         6713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.192, whole genome shotgun sequence.
ACCESSION   DADLSB010000192 DADLSB010000000
VERSION     DADLSB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.192"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            332..1987
                     /locus_tag="JN331_003681"
     CDS             332..1987
                     /locus_tag="JN331_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY7261692.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            2127..2351
                     /locus_tag="JN331_003682"
     CDS             2127..2351
                     /locus_tag="JN331_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY7261693.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            2414..2950
                     /gene="rimL"
                     /locus_tag="JN331_003683"
     CDS             2414..2950
                     /gene="rimL"
                     /locus_tag="JN331_003683"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY7261694.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(2945..3925)
                     /locus_tag="JN331_003684"
     CDS             complement(2945..3925)
                     /locus_tag="JN331_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAY7261695.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWLDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            4049..5041
                     /gene="tehA"
                     /locus_tag="JN331_003685"
     CDS             4049..5041
                     /gene="tehA"
                     /locus_tag="JN331_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY7261696.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            5038..5631
                     /gene="tehB"
                     /locus_tag="JN331_003686"
     CDS             5038..5631
                     /gene="tehB"
                     /locus_tag="JN331_003686"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY7261697.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            5934..6602
                     /locus_tag="JN331_003687"
     CDS             5934..6602
                     /locus_tag="JN331_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY7261698.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(6620..>6713)
                     /locus_tag="JN331_003688"
     CDS             complement(6620..>6713)
                     /locus_tag="JN331_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY7261699.1"
                     /translation="QQTPGQVENRALYPRPEGWGFTAHRIKSAI"
BASE COUNT         1718 a         1556 c         1642 g         1797 t
ORIGIN      
        1 ccagtcgcga agcatttgag caggcgttgg gttgtccggt tttggatgag ttccagcttt
       61 accaggaagc ggaagaacgc agtaaacgtc gctgtggttt gctttaaatt ctgaccatcc
      121 gcctttgcaa aaatttgcct gattttacaa acgaatcagg ctcatcccat cgacataaaa
      181 aaaatgccga tttatgcata ttctctcagt tcaacaattg gattattaat aaatattgtc
      241 tagagtgagc ggtcataaat aagcactttc ttgccgctga aaacgaccag cgcgggacca
      301 ttcacaacac cagaaggact cactttcagg tatggatcgt agacgattta ttaaaggttc
      361 aatggctatg gccgccgtgt gcggtaccag cggcattgct tctctttttt ctcaggcggc
      421 attcgcggca gattctgata ttgccgacgg gcaaacccag cgttttgact tctccattct
      481 acagtcaatg gcgcacgact tagcgcaaac agcgtggcgt ggtgcgcctc gtccgttacc
      541 tgacacgctg gcgacaatga cgccgcaggc ttataacagt attcaatacg acgccgaaaa
      601 atcgctctgg cataacgttg agaaccgtca actggacgct cagttcttcc atatgggaat
      661 gggattccgt cgccgcgttc gtatgttttc tgtagatcca gcaacacatc tggcgcgtga
      721 aattcacttt cgcccggagt tgttcaaata caacgatgca ggtgttgata ccaaacaatt
      781 agaagggcaa agcgatctcg gctttgccgg ttttcgcgtg tttaaagccc ccgaactggc
      841 gcgccgtgat gtagtatcat ttctcggcgc gagttatttc cgcgccgttg atgatacata
      901 tcaatacggt ttgtcggccc gcggcctggc gatcgacact tacaccgaca gtaaagaaga
      961 gttccccgac tttaccgcct tctggtttga tacggtaaaa ccgggggcaa ctacctttac
     1021 cgtttatgcg ttgctcgata gcgccagcat tactggtgcc tataagttca ctatccattg
     1081 tgagaaaagt caggtgatta tggatgtgga aaatcacctg tatgcgcgca aagacattaa
     1141 acagctgggc attgcgccga tgaccagtat gttcagctgc ggtactaatg aacgtcggat
     1201 gtgcgataca attcatccgc aaattcatga ctctgatcgt ctgtccatgt ggcggggcaa
     1261 tggcgagtgg atttgccgtc cgctgaataa tccgcaaaaa ttgcagttca atgcttacac
     1321 cgacaacaac ccgaaagggt ttggtttatt gcaactggat cgtgacttct cccattatca
     1381 ggacattatg ggctggtata acaaacgccc aagtctgtgg gtggaaccgc gtaacaagtg
     1441 gggtaagggc accatcggcc tgatggaaat cccaacaacg ggcgaaacgc tggataacat
     1501 tgtctgcttc tggcagccag aaaaagctgt aaaagcaggt gatgagtttg cattccagta
     1561 tcgtctgtac tggagtgcgc aaccgcctgt tcattgccca ttagcgcgcg ttatggcgac
     1621 gcgtaccggc atgggcggtt tcccggaagg ttgggcgcca ggtgaacact atcccgaaaa
     1681 atgggcgcgt cgttttgccg tcgatttcgt tggtggtgat ctgaaagctg ccgcgccaaa
     1741 aggcattgag ccggtgatta cgctttccag tggggaagcg aagcaaatcg aaattctcta
     1801 tattgaaccc atcgatggtt atcgtattca gtttgactgg tatccgactt cggactccac
     1861 tgatccggtc gatatgcgga tgtatctacg ttgtcagggg gacgctatca gtgaaacatg
     1921 gctgtatcag tatttcccgc cagcgccgga taaacgtcag tatgttgacg accgcgtgat
     1981 gagttaatcg ttttttcttc ggcaccttct tcgggaggtg ccgtctggtt aaacacgatc
     2041 ccgctcgcat ttttccctaa gttaaatgag taatctgatg gtgtgtattt cagatacacc
     2101 gtgtcagcca ctaacaggga gtgcgtatgt ttccagaata ccgagattta atatcccgtc
     2161 tgaaaaacga aaatcctcgc tttatgtcct tgttcgataa acacaataaa cttgatcatg
     2221 aaattgccag aaaggaaggt tccgacggtc gagggtacaa tgcggaagtg gtccgcatga
     2281 aaaaacaaaa gctacagtta aaagatgaga tgctcaaaat cctgcagcag gagagcgtca
     2341 aagaggtgta aactttccta agccgccagc caggcggctt ttttaacaac agcatggatt
     2401 gactggagat aagatgactg aaacgataaa agtaagcgaa tcacttgaat tacatgctgt
     2461 tgcggaaagt cacgtcacac ctctttatca gttaatctgt aaaaataaaa cctggttaca
     2521 gcagtcgcta aactggccgc agttcgttca gagtgaagag gacacgcgaa aaacggtgca
     2581 gggtaatgtg atgttgcatc aacgcggtta tgccaaaatg ttcatgattt tcaaagaaga
     2641 tgaacttatc ggcgttatct cgtttaatcg tattgaacca ctgaataaaa ctgctgaaat
     2701 cggctactgg ctggacgaat ctcatcaggg gcaggggatc atttctcagg cgctgcaggc
     2761 attgattcat cattacgctc agtctggtga acttagacgc ttcgtgatca aatgtcgggt
     2821 ggacaatccg caaagcaacc aggtcgcttt gcgcaatggt tttatccttg aaggttgcct
     2881 gaaacaggct gagttcctga atgatgccta tgatgatgtg aacttatacg cgcgtattat
     2941 cgattcataa taaccctgcc agcggcgttc gcgtaatgcg ctcctcgccg ttaatgactt
     3001 ttggaccacg gacatgaacc gtgtcgccat caaaggcttc aacgacggcg tggtcggtta
     3061 actccacatg gttttcaatt atcaccgcac ctgtaatacg gctctcacct tgtattacaa
     3121 cgtgctcgtc gagcagaatc ggtccaccac gtaccacggc atttccgcca atcaagacat
     3181 gatgttttaa cacacaatta ccttccacaa tggcatattc cgccacctgc gaactgtaat
     3241 gaatcgtcgg aatggcatct tcttctatgc cagctttcac ctgcgcgtga ccgtagactt
     3301 tagcgcaatc gcatagccag acgttgttct cttcattacc ttcaacactg gcaaaatcaa
     3361 aaacttcggc gcgatgttca ataaaagcat accggacgac ggcatcgcca taaatttgtg
     3421 cctggtggac aatacgcgag gcgctaaccc ttgcgcgatc ataaatttgg agcaggagtt
     3481 gatggtcggg cgttaagcct tgtgcggcga cgatcataga atgttgatca attaaggcgt
     3541 gaccaaatat tctacattgc ccgtaaacta atgagtcatg aatggtgaca ctgtcactta
     3601 tatatgcacc ctggctgatt tcactattat cgatccagac gttatccgtt gcgtaaacct
     3661 ctccccacag cacgctggtg ccggtgatac gcgtattacc ggaaatcact gttccgccga
     3721 acgcaatagc attctggtcg taaatccagc aatttccttc ctgtgctaac accgtttcgc
     3781 gatcgagcca gccgccagcg gttcctgcta tcacatcgtt aaaatcgcta atggcgataa
     3841 tctgccgtaa taacacactt tttttagtgc catcttcctg ataactaaag gctcgctgtt
     3901 cttcacttag gcgatatttg cgcatagcgt tttcccacag gtgacttact ataaccgtag
     3961 caaattctgc ggctctggct atgctcgaga aaattccata aaatgcattt caaatatact
     4021 ttataaatta aacaaaatga gtaagaagat gcagagcgat aaagtgctca atttgccggc
     4081 aggctacttt ggtattgtgt tggggacgat agggatggga tttgcctggc gctatgccag
     4141 ccaggtttgg caggtcagcc actggttagg ggatgggctg gtgattctgg cgatgatcat
     4201 ctggggatta ttgactagcg catttattac ccgactcata cgctttccgc atagcgtgct
     4261 ggcggaagtt cgccatccag tgctgagcag ttttgtgagt ttgtttccgg caacgacgat
     4321 gctggtggcg attggttttg ttccgtggtt tcgcccactg gcggtgtgca tgttcagttt
     4381 tggtgtcgtg gttcagttgg cttatgccgc ctggcaaact gcgggattat ggcgcggatc
     4441 tcaccctgaa gaagctacca cgcctggact gtatctgccg acagttgcca acaactttat
     4501 cagcgcaatg gcctgtggtg cgttgggcta caccgacgcc ggtctggtgt ttttaggcgc
     4561 aggcgttttc tcatggctaa gccttgaacc ggtgatcttg cagcgtctgc gcagttcggg
     4621 agaattaccc acggcactgc ggacatcact cggcattcag ctcgctcctg cgctggtggc
     4681 ctgtagtgcc tggctgagcg tcaacggcgg cgagggtgac acgctggcga aaatgctttt
     4741 cggttatgga ctgctgcaac tgctgtttat gctacgtctg atgccatggt atctctccca
     4801 gccatttaat gcttcattct ggagtttctc gttcggcgta tctgcactgg caaccaccgg
     4861 tttgcatctg gggagtggca gcgataatgg atttttccat acgctggcgg tgccgctgtt
     4921 tatctttacc aattttatta ttgcaatact gctcatccgt acttttgcgc ttctgatgca
     4981 gggaaaattg ttagtcagaa ccgagcgcgc cgttttaatg aaagcagagg acaaagaatg
     5041 atcattcgtg acgaaaacta ttttactgat aaatatgaat taacccgcac acactctgaa
     5101 gtactggaag cggtgaaagt ggttaaaccg ggtaaaacgc tggatctggg ctgtggcaat
     5161 ggtcgtaaca gtctttacct ggcagccaat ggttatgatg ttgacgcatg ggataaaaat
     5221 gccatgagta tcgccaacgt cgagcgcatt aaatccattg aaaatctgga taatttacac
     5281 acccgggtcg ttgatctgaa taacctcaca tttgatggac agtacgattt tattctttcg
     5341 actgtggtgc tgatgttcct tgaggctaaa accatcccag ggttgattgc caatatgcaa
     5401 cgttgcacta aacctggtgg ttacaacctg attgtggcgg cgatggatac cgctgattat
     5461 ccatgtaccg tcggcttcac gtttgccttc aaagagggag aattacgtcg atattacgaa
     5521 ggctgggaga gggtgaaata caatgaagac gtcggcgagc tgcaccgcac cgacgccaac
     5581 ggtaatcgta ttaaactgcg tttcgccacg atgctggcac gtaaaaaatg acccggtaag
     5641 cacaaaacgc gtgaaaattc cccacgctga gatgatttac tgttcttctt ttcggtaagc
     5701 atatttttta tcgaagggat gtgaaattaa tcacagtagt cgaagttttt tagcagctta
     5761 acttactgaa atttaactac tgatgattga cttagcccct ttttcggcat tgactatgtc
     5821 gtctgaaaag gggctgaaaa atttattttc accaacactt tttttgccac aacacgaagc
     5881 ggcgcttttt gctataactt agaaagtaat ataatcatct caggaaacta ttcatgcgta
     5941 ccacatcatt tgcgaaagtt gcagctttat gcggcttgtt ggctctgtct ggttgtgcct
     6001 ctaaaatcac ccagccagat aaatattctg gttttttaaa caattactct gatttaaaag
     6061 aaacaacctc agcaacaggt aaacctgttt tacgttgggt agacccgagt tttgatcaaa
     6121 gcaaatatga cagcatcgtc tggaacccaa tcacttatta tccggtacca aaaccttcta
     6181 cccaggtagg gcagaaagtt ctggataaaa ttttgaacta taccaacacc gaaatgaaag
     6241 aagcgatagc gcagcgtaaa ccagtggtta ccaccgctgg gccgcgtagt ctgattttcc
     6301 gtggggccat taccggtgta gataccagca aagaagggct gcaattctat gaagtggttc
     6361 ctgttgcatt agtggttgcg gggacgcaaa tggctacagg ccaccgtacc atggatactc
     6421 gcctctattt tgaaggtgag ctgattgatg cagcgactaa taaaccggtt atcaaagtcg
     6481 ttcgtcaggg cgaaggtaaa gacctgaata acgaaagtac gccaatggct ttcgaaaata
     6541 ttaaacaagt tattgatgac atggcgaccg atgccaccat gtttgacgtt aacaaaaagt
     6601 agttccagac gcgccatcgt tagatggcgc tttttatccg gtgcgccgta aaaccccatc
     6661 cttcagggcg gggatataag gcgcggtttt ccacctgacc aggtgtttgc tgt
//
LOCUS       DADLSB010000193         6641 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.193, whole genome shotgun sequence.
ACCESSION   DADLSB010000193 DADLSB010000000
VERSION     DADLSB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.193"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(1..800)
                     /locus_tag="JN331_003689"
                     /pseudo
     CDS             complement(1..800)
                     /locus_tag="JN331_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(810..1601)
                     /locus_tag="JN331_003690"
     CDS             complement(810..1601)
                     /locus_tag="JN331_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAY7261700.1"
                     /translation="MKSTRPVAVITGAARGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1813..2460
                     /locus_tag="JN331_003691"
     CDS             1813..2460
                     /locus_tag="JN331_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7261701.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKVIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            complement(2444..2989)
                     /locus_tag="JN331_003692"
     CDS             complement(2444..2989)
                     /locus_tag="JN331_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261702.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            3208..3828
                     /locus_tag="JN331_003693"
     CDS             3208..3828
                     /locus_tag="JN331_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7261703.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4136..5548
                     /gene="cycA"
                     /locus_tag="JN331_003694"
                     /gene_synonym="dagA"
     CDS             4136..5548
                     /gene="cycA"
                     /locus_tag="JN331_003694"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY7261704.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5593..6255)
                     /gene="ytfE"
                     /locus_tag="JN331_003695"
     CDS             complement(5593..6255)
                     /gene="ytfE"
                     /locus_tag="JN331_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY7261705.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6363..>6641)
                     /locus_tag="JN331_003696"
     CDS             complement(6363..>6641)
                     /locus_tag="JN331_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HAY7261706.1"
                     /translation="PLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETL
                     AGLLYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
BASE COUNT         1538 a         1652 c         1731 g         1720 t
ORIGIN      
        1 ctgttaatat ctttctgcca ccagtaaggc gagaatattt ccagttcaat agcaccatta
       61 aatccggcgt tttcaaccag ccgacgaatt gacggaatat tgatgacgcc atctcccggc
      121 ataccacggt cattgaccag atcagtggtc ggcactaacc agtcggaaac atgaaacgcg
      181 aggatgcgtt ttccggcacg caggatctga ctggcgagat ccggatccca ccagacgtga
      241 taaacatcca ccgccacgcc gaggccaaac tcaccatcgg gatcgagttc atcacaccag
      301 tccagcgcct gacgtaacgt gcacaggcaa gagcgatcgg cagctgtcat cgggtgcagt
      361 ggctccagcg caattggcaa gccgacatct ttcgaatgtg gcagcaactg gcgaatccct
      421 tgtttcactt gttctcgcgc ttcatataga tctttagtgc ccgttggcag accgccaacc
      481 acctgcatat aacaggcggc gttaagcacc gctgcgtcgt ccagcgcccg gcggttatca
      541 tcaatcgcca gtttgcgttc cgccagtgtc ggcgcggtgt agtaagtgct acggcacaaa
      601 ccggaaacgc tcatgttact ggcggcaagc tggcgggtga tttgctggag atcttcaccc
      661 tacaactccc tgcgccaggg ggcaattgcg ccaataccgc gtgcggcgca ggcatcaata
      721 atggcgggta gcggcgtttt atagcccagt gtcgcggtat tgatactgaa taaatccggg
      781 cgttgtaaca gatctctcat gattcagcct cagaagcgcg gcatacttaa gccgccgtcg
      841 attgcgaccg cctgaccaca ggtgtaaatc agtttacctt ccgccatcgc gcggacggtg
      901 gaagcaatat cagccggata accccaacgt ccccacggca ccagaccttt ggcaatcagt
      961 tcgtcgtaat aagccgttgc cggaatagtc atatcagttt tgatgagacc agggcgcact
     1021 tcataaacgc caatttgttc attacaaagg cgagcagcaa acagacgagc agcggcagaa
     1081 acggcggttt tggcaatggt gtattcgccg cgattcatcg ccagcatgat ggcgttgatg
     1141 gaactgacga aaataatcga gcggtgcggc agctccgctt ccggtttcgg ctgtgccagt
     1201 aagcgcttac tgaatgcctg agcaaggaag aacggcgcgc gggtgttaat ggcaatgttt
     1261 tgatcgaagc tgtctggttc gaggtcgagg aggtcaccgc gttttttcac tgaaatcccc
     1321 gcgttattga gcagacagtc cagccgcccc cactggtttt gcgccgcgtc gagcatctcc
     1381 tcatgcagag atagatcacc gacatcagca gggaagcaaa tcacttccac gccttccgcc
     1441 aaacattctt gttgtgtgat atgcagtttt tctacgctgt cggcatcggg acgatcgtta
     1501 atcagcaggt taaacccgcc acgagcaagc tcctgcgcac agccctttcc aataccgcga
     1561 gcggctccgg taatcacggc aacaggacga gtggatttca tacaatagct ctcttttttg
     1621 aattttgttt ccttggtgat gattcgcttg cgactggagg ataaaaagac caaccatcta
     1681 gttggttgat atgcgatggc gatcacagga catttgataa cctgatgaaa gtacaagttt
     1741 ttgccctttt ttctgaagct gaaatcaaat caggttatga tagtcaccac gaagaggtcg
     1801 tggggaagcg ttatgtatat tgataccagc aacgtacaga gcctgaagga aaaattgctg
     1861 ctatgtgcgg ttaatgaatt tgccgaatat ggttatgaag gggccagagt agataatatt
     1921 gtaaaggcag cagggtgtag taaacagacg gtttatcacc actttggtaa taaagaaaat
     1981 ttatttatcg aagtgctgga atatacctgg aacgatattc gacaaaaaga aaaggccctt
     2041 gatttctcag atcttccgcc acaaaaagcc attgagaaag ttattgattt tacatgggat
     2101 tattatattg ctaatccttg gtttttaaaa attgtgcata gtgaaaatca aagtaaaggt
     2161 gtgcattatg caaaatcaca gcgtctctta gaaattaacc atgcgcactt gcagttaatg
     2221 gaatccttat tggatgaagg gaagaaatac aatattttta agccaggtat cgacccattg
     2281 caggtgaata ttaatattgc tgcgcttggg ggatattatt tgatcaacca gcatacgctt
     2341 ggcctggttt atcatatcag tatggtttcc ccccaggcac tggaagccag acgtaaggtc
     2401 atcaaagaga caatccttag ctggcttttg gttgaccctt catctaccgc gcacgaataa
     2461 aactgccatc cggctggcgg gtaaacagga cctgttgatt attccccgta tcaatggtta
     2521 agcccgtcac cacgccgctg gcgttctggc ggattttcac catctgccca ttttgcaaat
     2581 tactcagcgg cttacccgcg ccttctacct gcgccattgc atagacatcg gtagcgggca
     2641 gcccgtggtc gcggaacagc tgcgccatcg ttttaccggg ttcgacacgg taagagcgcc
     2701 attgattatc aattccgcta tctggctgga atggttgcgt ttgggtagtt tgcggctgtt
     2761 cttctgtcgg cggttgtgat tgcgactgaa tatctaactg cgcttcacgc gtcaccaccg
     2821 gcgcgttggg cgtatcatca gatggaagca gaaatccgac caccaataca atggcggcaa
     2881 taatgatccc ccgacgatgc atcggcggca gcgggtccat gaagcgaaaa ttgtccggcg
     2941 cgtgccagac tttctccagg gttggtttta gttcaaagcg cccgggcatg gtttcctcct
     3001 gctccacgtc ttgttcctca atcatagcct atgaataagc taacgctatg atgtccgtgg
     3061 taaacccgcc tttattatat tcatacggga tattgctatt gtttcttttt ccccgggatt
     3121 tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt ggctgatatg ctgcccgcta
     3181 cttttatacc ctaagaaagg aaatacgatg accaccccaa cttttgacac catcgaagcg
     3241 caagcaagct acggcattgg tttgcaggta gggcaacaac tgagtgaatc tggcctggaa
     3301 gggctgctgc cagaagcact ggttgcaggt attgccgatg cgctggaagg caaacatccg
     3361 gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa tccacgaacg cgccgatgcc
     3421 gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg tgaaatacct ggaagaaaac
     3481 gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc aattccgcgt gatcaaccag
     3541 ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg ttcattacac cggtaaactg
     3601 atcgacggca ccgtgtttga cagctccgtt gctcgtggtg aacccgctga attcccggtt
     3661 aatggcgtga tccctggctg gattgaagca ctgactctga tgccggtagg ttctaaatgg
     3721 gaactgacta tcccgcagga actggcatat ggcgagcgcg gcgcaggtgc atccatccct
     3781 ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa tcctctaagc agcgcattct
     3841 gttcccctcg aaccagaggg gagcaggcat tcagcaataa acccttcagt ttgccaaacg
     3901 gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt ttgtgagctg tttcgcgtta
     3961 tcaccgtgat atgacactca ctttaaacat aaaattaaca ttagatctaa atcttagtat
     4021 tcatcccgcg tattgttacc taatatcgat gagtcccgat acagattcgt cgtatcatag
     4081 actgactaaa ggccgtagag cctgaacaac acagacaggt acaggaagaa aaaacatggt
     4141 agatcaggta aaagtcgttg ccgatgatca ggctccggct gaacagtcgc tacggcgcaa
     4201 tctcacaaac cgacatattc agcttattgc cattggcggt gccattggta ctgggttgtt
     4261 tatggggtcc ggcaaaacga ttagccttgc cgggccgtcg atcattttcg tttatatgat
     4321 cattggtttt atgctctttt tcgtgatgcg ggcaatgggg gaattgctgc tttcgaatct
     4381 ggaatacaaa tcttttagtg acttcgcttc cgatttactg gggccgtggg cgggatattt
     4441 caccggctgg acttactggt tctgctgggt tgtaaccggt atggcagacg tggtggcgat
     4501 cacggcttat gctcagttct ggttccccga tctctccgac tgggtcgcct cgctggcggt
     4561 gatagtgctg ctgctgacgc tcaaccttgc caccgtgaaa atgttcggtg agatggagtt
     4621 ctggtttgcg atgatcaaaa tcgtcgccat cgtgtcgctg attgtcgtcg gcctggtcat
     4681 ggtggcgatg cactttcagt caccgaccgg tgtggaagcg tcattcgcgc atttgtggaa
     4741 tgacggcggc tggttcccga aaggtttaag tggcttcttt gccggattcc agatagcggt
     4801 tttcgctttc gtggggattg agctggtagg tacaacagct gcggaaacca aagatccgga
     4861 gaaatcactg ccacgcgcga ttaactccat tccgatccgt atcattatgt tctacgtctt
     4921 cgcgctgatt gtgattatgt ccgtgacacc gtggagttcg gtagtcccgg agaaaagccc
     4981 gttcgttgag ctgtttgtgt tggtaggttt gcctgcggct gccagcgtga tcaactttgt
     5041 ggtgctgacc tctgcggcgt cttccgctaa cagcggtgtc ttctctacca gccgtatgct
     5101 gtttggtctg gcgcaggaag gtgttgcgcc gaaagcgttc gctaaacttt ctaagcgcgc
     5161 agtacccgcg aaagggttga ccttctcgtg tatctgtctg ctcggcggcg tggtgatgtt
     5221 gtatgtgaat cctagcgtga ttggcgcgtt cacgatgatt acaaccgttt ccgcgattct
     5281 gtttatgttc gtctggacga ttatcctttg ctcgtacctg gtgtaccgca aacagcgtcc
     5341 tcatctgcat gaaaagtcga tctacaagat gccgctcggc aagctgatgt gctgggtatg
     5401 tatggcgttc tttgtgttcg tggtcgtgtt gctgacactg gaagatgaca ctcgccaggc
     5461 gctgctggtt accccgctgt ggtttatcgc gctggggttg ggctggctgt ttattggtaa
     5521 gaagcgggct gctgaactgc ggaaataacc gcattatcat gctggatggc gcaatgccat
     5581 ccagctttta gatcactcac ccgctagggc gcgtgggaac agtacattgt tttccagact
     5641 gatgtgatcc atcaggtcat caatcagttc attaatgccg ttatacatcg ctttccaggt
     5701 ggtgcaggct tctggcggcg gtgtgacgtt attggtagtg tgtttgatca cttccagcag
     5761 ttcgcccgct tcatcgtgct cgctttccat tacgctgatt ggccccattg cctggctgcc
     5821 catgccttgt ttgatcatcg ggaagaggat ctgctcttct ttcatcatgt ggctggaaag
     5881 ctcttcatgc agcatggtca ggtattttgt cagccctttt ggcacgctcg gtttgtcggc
     5941 gtgaacgcgc tcgactttag tcgcttgcag gatcagctcc ggcagctgct cgcggtgacg
     6001 atcgtggtag cgcacgatga tatggtcaat gatttctgcc agcggggcgc tacgccagtc
     6061 tttctcaatc ggttgttcag caagctttgc cagttcagct tcaatgacct caacatccag
     6121 ttctttacgt gccgccgcgc gcgccagcgt ctgcttaccg ccacagcagt aatccatatc
     6181 atatttacga aacagagccg aagcgcgagg gatagagagc gccagttcac ctaaaggttg
     6241 gtcgcgataa gccatagctg atacctcatt cttaataaga taagatgtat tttaaataca
     6301 cctttaaggc aaaagctata acccttacgt agcagaggct tatttaactc actgccagca
     6361 tgtcaacttt ctgagacaga ttcagttaaa ggtttttctg gttttgctct gaccgcgatg
     6421 accacgccaa tcactaacag cgcgataccg ctcagcgtca ttagcggcgg catttgctgg
     6481 cggagtaaaa aggtgtacag caaacctgcc agcgtttcga aaacaatcag cggcccgaga
     6541 atcactgtcg gtaatcgctg gctggcgacg ttccagcaga gtgcgccaac ccatgagcaa
     6601 agcacggcta tcgcaaccat cagactaata aacaccagcg g
//
LOCUS       DADLSB010000194         6546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.194, whole genome shotgun sequence.
ACCESSION   DADLSB010000194 DADLSB010000000
VERSION     DADLSB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.194"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..231
                     /locus_tag="JN331_003697"
     CDS             <1..231
                     /locus_tag="JN331_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7261707.1"
                     /translation="IGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALK
                     MPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            320..1780
                     /locus_tag="JN331_003698"
     CDS             320..1780
                     /locus_tag="JN331_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY7261708.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKTAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GNDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1816..2019)
                     /gene="ydfZ"
                     /locus_tag="JN331_003699"
     CDS             complement(1816..2019)
                     /gene="ydfZ"
                     /locus_tag="JN331_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY7261709.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2197..2883)
                     /locus_tag="JN331_003700"
     CDS             complement(2197..2883)
                     /locus_tag="JN331_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7261710.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2972..3718)
                     /gene="ydfG"
                     /locus_tag="JN331_003701"
     CDS             complement(2972..3718)
                     /gene="ydfG"
                     /locus_tag="JN331_003701"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY7261711.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3855..5900
                     /gene="dcp"
                     /locus_tag="JN331_003702"
     CDS             3855..5900
                     /gene="dcp"
                     /locus_tag="JN331_003702"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY7261712.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDEFASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5945..6463)
                     /locus_tag="JN331_003703"
     CDS             complement(5945..6463)
                     /locus_tag="JN331_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HAY7261713.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1625 a         1563 c         1667 g         1691 t
ORIGIN      
        1 attggtggtc ttatcgcttc cggttttggt cctatcctag cgggtatttt ctgcaccatg
       61 acggaatcct ggtatccgat cgccattatg atcatggcat attcagtgat tggtttaatc
      121 tctgcgctga aaatgccaga ggtgaaagac cgtgatttaa gtgcgctgga agacgctgcg
      181 gaagatcaac cgcgtgttgt aagagctgcg caaccttcca gaagtctgta aaccctcaat
      241 cccttctctt accggagagg ggatttttat tcatataaca aaacatatag cttgccatat
      301 ttaaatttaa ggaattgtta tgggaaataa tttgttatca gcaaaagcga cactccctgt
      361 ttatgatcgt aataacctgg ccccaagaat tgttcattta ggctttggtg catttcaccg
      421 tgcgcatcag ggcgtgtatg ccgatattct tgctacagaa catttcagtg actggggata
      481 ttatgaggtc aacttaatcg gcggcgaaca gcaaattgcc gatttacaac agcaagataa
      541 tctttatacc gttgcggaaa tgtcggccga tgcgtggacg gctcgcgtcg ttggcgtcgt
      601 taaaaaagcc ttgcacgtac agatggatgg cttagaaacc gtgttggctg cgatgtgtga
      661 accgcaaatc gcgattgtct ctctgacaat caccgaaaaa gggtatttcc actctccggc
      721 gaccggacag ttaatgctcg atcacccgat ggtcgctgcc gacgtgcaaa atccccacca
      781 gccgaaaaca gcaacagggg tgattgttga ggcgctggct cgccgtaaaa cggcaggact
      841 tcccgcattt accgtcatgt catgtgacaa catgccagaa aacggtcatg ttatgcgtga
      901 cgttgtcact tcctacgcgc aagccgttga tgtaaaactg gcacaatgga tcgaagataa
      961 cgtgactttc ccatcaacaa tggtggaccg tattgtgccc gcagtgacag aggatacgct
     1021 ggcgaaaatc gaacaactta ccggtgtgcg cgatcctgct ggcgttgcct gtgaaccttt
     1081 ccgccagtgg gttatcgaag ataactttgt agccggacgt ccggaatggg aaaaagcggg
     1141 agccgaactg gttagtgatg tgctgcctta tgaagagatg aagttgcgca tgctcaacgg
     1201 cagtcattca ttcctggcat atctgggtta tcttgctgga tatcagcaca ttaatgactg
     1261 tatggaagat gaacattatc gtcatgcggc gtatggcttg atgttgcagg aacaagcgcc
     1321 gacgctgaaa gtgcagggcg ttgatttgca agattacgct aaccgattaa ttgcacgcta
     1381 tagcaacccg gcgttacgtc atcgaacctg gcagattgcg atggatggca gccagaaatt
     1441 gccacagcgg atgttggatt ctgttcgctg gcatctggcg catgacagca agttcgatct
     1501 gctggcgctg ggcgtcgcgg gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa
     1561 tccgatagaa atcagtgacc cactgttacc tgttattcag aaggctgtac aaagtagtgc
     1621 cgaagggaaa gcgcgcgtcc agtcattgct ggcgattaag gcaatctttg gtaatgattt
     1681 gccagacaat agcttgttta ctgcaaaagt gacggaagcg tacttgtctt tattagcgca
     1741 tggcgcgaaa gcgaccgtgg cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg
     1801 gcggcattca cacacttagt tcatgccgag acgaatcagg tccagaggtg ccagtttctc
     1861 ttcacaacct tcaacaacta cggtttttcc gcggcggatt tgctcagcgg tcagaccttc
     1921 agtatcaatc gacaggatct tgccagtgtg accggtgccg ctaaccataa cacggctgcc
     1981 agtggtgatt gcgttacggt tacgatcgta ggtcgtcata ggtgttttct cctttctgat
     2041 ttacagtcga agcccacgta acgggcgcaa tataaatact cctgtcgagt gaaatatttt
     2101 ttgtttttga tcaagctcac atcttttttg atgcatgcat gagccgtaag gcggggggaa
     2161 aagcctctca tcccggatgg gatgagagta aatgaattac tcttcgctaa accagtcgct
     2221 gttttcctgg cggatctggc gcacggattc gctgatttcc tgcaaatgtt gcgtcattgc
     2281 acgttctacc gcatcgccat cacgtttttg cagggcagag aaaatatcaa gatgctggcg
     2341 taacagcatt tctggtggag aaacatggtc gaaactcata tagcgcacgc gatcaacggt
     2401 cgctttcagg ttctcaatgg tatcccacgc cagttgacag tcggcaatct gcgtcaggag
     2461 ttgatggaag ttgtcatcaa gttcaaaaaa atcatccagt tgcttgcgct caatggcaat
     2521 gcgttgctgg tgaagatttt gttccagttg gtagcactgg ctttcggtaa tcatgctcgc
     2581 cgcccgacgc gccaccgcgc actcaatggc ctgacggata aaactgccgt tgcgcacctg
     2641 ggccatggaa attttgttga cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt
     2701 ttccgccagt ttaataaagg cttcacgaac cggctggcgt gacacattga aacgaacaga
     2761 aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg
     2821 aagaatacga taaatctgct gattgacagg ctgtgtggga ttaagttgcg tttcgacggt
     2881 cattcgttct tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc
     2941 aataacccgg cagttacgcc gggtataaaa attactgacg gtggacattc agtccggcat
     3001 agctttgggt aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg
     3061 acacccacca gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg
     3121 ttttttccgc tttaccgtca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca
     3181 ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc atgcagatcc gtacgcagat
     3241 tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcatacg
     3301 gccagctgcc tgccgttgag ccaatgttaa taatatgacc atgattacgt tcaaccatac
     3361 ccggtaagac ggcgcgcgtc atatatacta ggcctttgtt gttggtatca atcatcgttt
     3421 cccagtcttc aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat
     3481 ttaccaggat atcaatattg ctccactcgg cagggagcga tgccagcatc tcttcaatag
     3541 cggcgcggtt gcgaacgtcc agttgggcga tatacagatt atctcccagt tcgtctttta
     3601 actcctgcaa ccgctcctgg cgacggccag tggcgataac tttatgccct tgttgaataa
     3661 aacgacgagt aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct
     3721 cactgttcct caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta
     3781 atactggttt gcaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg
     3841 ttcaggagaa gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc
     3901 tggctcctca ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa
     3961 tgcaacaaaa gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg cctgatttca
     4021 acaatactat tctggcactg gaacaaagcg gagaattact tacccgcgtt accagcgtct
     4081 tttttgcgat gactgcggcg cataccaatg atgaattaca gcgtcttgat gaacagtttt
     4141 ccgctgaact ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg
     4201 tagatgctgt ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg
     4261 tggaggttat tcatcaacgt tttgtccttg ccggagccaa acttgcacaa gctgataaag
     4321 caaaattaaa agtactgaat acagaagctg cgaccctgac cagccagttt aaccagcggt
     4381 tacttgcagc aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa
     4441 tgagtgagca agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca
     4501 aatggctgat tccgctgctg aataccaccc aacaaccggc gcttgccgaa atgcgcgatc
     4561 gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatgcca
     4621 atgatacccg cgctatcatt caacgtctgg tagagattcg cgcacagcag gcgaaactgc
     4681 ttggttttcc tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acgccagaag
     4741 cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat
     4801 ttgcctccat acaggcggtt atcgataagc agcagggcgg gtttagcgcg cagccgtggg
     4861 actgggcatt ttatgcggaa caggtacggc gggagaaatt tgatcttgat gaggcgcagc
     4921 tcaagccata ttttgaatta aacacggtgt taaatgaagg tgtattctgg accgcgaatc
     4981 agctcttcgg tattaagttt gtcgaacgtt ttgatattcc ggtctaccat cctgacgttc
     5041 gtgtgtggga aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct
     5101 tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc
     5161 ttaatgaaac gcatccggta atttataacg tctgcaatta tcagaaaccc gctgccggtg
     5221 agcctgcgtt gttactctgg gatgatgtaa taaccttatt ccatgaattt ggtcatacgc
     5281 tgcacggcct ttttgcccgc cagcgttatg ccacgctttc cggcaccaac acgccgcgtg
     5341 attttgtcga atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg
     5401 ctcgctacgc ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa
     5461 tgcgtaatgc cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac
     5521 ttctcgatat gcgctggcat tgcttggaag aaaacgaagc aatgcaggat gtcgatgatt
     5581 ttgaattgcg ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc
     5641 gcagcagtta tttcgcccat atttttggtg gcggatatgc tgcaggttat tacgcttatc
     5701 tgtggacgca aatgttggcc gatgatggtt accagtggtt tgttgagcag ggcggattaa
     5761 cgcgtgaaaa tgggcagcgt tttcgcgagg cgatcctttc cagaggtaac agcgaagatc
     5821 tggaacgcct gcatcgacaa tggcgcggta aggctcctca gattatgccg atgctgcaac
     5881 atcgtggctt gaacatataa acaccaccaa tggctggcct ggagatatcg ccaggccagc
     5941 ctggttatag cagtaattgc agcagctggg cgagggcaaa cctcaccgct aaagctaata
     6001 ccgtttcgca gtcgccagca aaatgcataa ccgcagtgta gttctggcct ttaatatgcc
     6061 acgcgaacca gacggtaccc gctggcgtac catcttcacc gccctccggt ccgccgtagc
     6121 cggtaatggc aatactgaca tcagcatccg cacgctctat ggcacctgtt gccatttctg
     6181 ctgccacttt ctcactcacc gcagaatatc gttcaagaga ctgctggctt acgctgagga
     6241 ttttcatctt tgcctgatcg gtgaaagtaa caaagcctgc accgtaaaat ttgggtgtat
     6301 cttcagctgc acacagggcg ctagccaact taccgccggt gcatgattcg gcggtagtta
     6361 agcgtaattg atccgcgata agtctttgac tcaacgcgga tgtcaggttt tcaatagtgt
     6421 cggtatctgc gagttgtaca attttatctt tattaatgtt cactgttttt tgtccacgct
     6481 attatatgta aaggtgattt gctttttctg tatgctcgtc caatataata attttcaaac
     6541 atataa
//
LOCUS       DADLSB010000195         6508 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.195, whole genome shotgun sequence.
ACCESSION   DADLSB010000195 DADLSB010000000
VERSION     DADLSB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.195"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..476)
                     /locus_tag="JN331_003704"
     CDS             complement(<1..476)
                     /locus_tag="JN331_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY7261714.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYK"
     gene            665..2263
                     /locus_tag="JN331_003705"
     CDS             665..2263
                     /locus_tag="JN331_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY7261715.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(2245..3003)
                     /locus_tag="JN331_003706"
     CDS             complement(2245..3003)
                     /locus_tag="JN331_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY7261716.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(3020..3850)
                     /gene="glpG"
                     /locus_tag="JN331_003707"
     CDS             complement(3020..3850)
                     /gene="glpG"
                     /locus_tag="JN331_003707"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY7261717.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAGLARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3895..4221)
                     /gene="glpE"
                     /locus_tag="JN331_003708"
     CDS             complement(3895..4221)
                     /gene="glpE"
                     /locus_tag="JN331_003708"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY7261718.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            4411..5916
                     /gene="glpD"
                     /locus_tag="JN331_003709"
     CDS             4411..5916
                     /gene="glpD"
                     /locus_tag="JN331_003709"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY7261719.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(6185..6415)
                     /locus_tag="JN331_003710"
                     /pseudo
     CDS             complement(6185..6415)
                     /locus_tag="JN331_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         1578 a         1746 c         1678 g         1506 t
ORIGIN      
        1 tgtttatagt tgttagtgtt aaggaaacgc ggatattttt gcgttaacca ctgataaccg
       61 gcctccagct cagcccattt cgcatcgacg ttaaaaggcg ggttttccca tgcgccttta
      121 tcacgtttac aacgcccggt agtacgcccg tgtggtacgg ccgtttcaat cgcttgacgc
      181 agctccgcca ggttttcagg caggtctgcc gccgttaacg cggtacgcag cgcgttcatt
      241 ccacagccaa tatccacgcc caccgccgcc ggaataatcg cccctttggt cgggatcacg
      301 ctaccaatgg tggaaccttt acccaggtgt acatcaggca ttaccgcaat atgtttgaaa
      361 ataaacggca tcttcgccgt attaataagt tgctgacgcg catcggcttc taccggcacg
      421 cctttggtcc acatttttac cggggcattt tcagtggtca gtaattcgta attcattttg
      481 tttttctctt ttcgttgttt gcggtcctga taattgcaac cgtcgtgcca gaaaattaaa
      541 taagcagatt aattttttaa tttgttgttt ctaaaaagat tatatcttta tgtccgtaaa
      601 cggagatttc ccgcaaagcc gatttaccga taatgaaata tcgcctttta taaggatatc
      661 taagatgcgt aaaacagtgg cttttggctt tgtcggtacc gtgctggatt atgccggacg
      721 cggcagtcag cgctggtcaa aatggcgtcc gtcactctgt atatgccagc aagaatcgtt
      781 ggtcatcgat cgactggaat tattgcacga tgcccgctcg cgctcgctat ttgaaacgct
      841 taaacgcgat atcgccagcg tttcgccaga aacagaagtg gtgggcgttg agattgaact
      901 gcataacccg tgggatttcg aagaggttta cgcctgcctg catgatttcg cccgtggtta
      961 cgagtttcag ccagaaaaag aagactattt aattcacatc accaccggca cccacgtcgc
     1021 gcagatttgc tggtttctgc tggcggaagc gcgttatcta cccgcccggt tgatacaatc
     1081 ttcaactccg cgcaaaaaag aacagccgcg cggcccaggt gaagtgacga ttatcgatct
     1141 cgatttaagc cgttataacg ccatcgccag ccgctttgcc gaggaacggc agcaaacgct
     1201 cgatttcctc aaatccggta ttgccacgcg taacccccac ttcaaccgca tgattgagca
     1261 gatcgaaaaa gtggcgatta aatcccgcgc gccgattctg cttaacggcc cgaccggcgc
     1321 aggtaagtca tttctggcgc gacgcatctt cgagttaaaa caggcacggc atcagtttag
     1381 cggcgcgttt gtggaagtga actgcgccac cctgcgcggc gataccgcga tgtcgacgct
     1441 gtttggtcat gtgaaaggcg cgtttaccgg ggcgcgggaa tctcgtgaag gattattacg
     1501 cagcgccaac ggcggaatgt tgtttcttga tgagattggc gaactgggcg cagacgaaca
     1561 ggcaatgctg ctgaaagcta ttgaagagaa aaccttttac ccgtttggca gcgatcgcca
     1621 ggtgagcagt gattttcagc ttatcgccgg aacggtgcgc gatttgcgcc agctggttgc
     1681 cgaaggcaaa ttccgcgaag atctgtacgc gcggatcaat ctctggacct tcaccctgcc
     1741 gggtctacgc cagcgccagg aagatattga accgaacctg gattatgaag tggagcgcca
     1801 cgccacactc actggtgaca gcgtgcgttt taacaccgaa gcgcggcgtg cctggctggc
     1861 ctttgcaaca tctccccagg caacatggcg cggtaacttt cgcgaacttt ctgccagcgt
     1921 cacgcggatg gccacctttg ccactagcgg acgcatcact ctggacgtgg tggaagatga
     1981 gataaaccgt ctgcgctata actggcagga gagccgcccc tccgcgctta cggcgttgct
     2041 gggcgcagag gcagaaaaca tcgatctctt cgaccgtatg caactggaac acgttatcgc
     2101 tatctgccgc caggcaaagt cgctttctgc tgccggacga cagctttttg atgtttcgcg
     2161 ccagggcaaa gccagcgtca atgacgcgga tcggctacgc aaatacctgg cgcgttttgg
     2221 tctgacgtgg gaagccgtgc aggatcagca cagctccagt tgaatatggt ggtccgtcag
     2281 cacctgcatc acgcttactg gcggcggggc gtcggtgtag acggcatcaa ccatgctgat
     2341 gctccccata ttgaccatcg cattacggcc aaattttgaa tggtcaacca ccagcataac
     2401 gtggcgcgag ttctcaataa tggcgcgctt ggtacgaact tcgtggtaat cgaactccag
     2461 cagcgagccg tcgctgtcga tgccgctgat ccccagaatg ccgaaatcaa ggcggaactg
     2521 ggagataaaa tcgagcgtcg cttcgccaat gatcccgcca tcgcggctac gtaattcgcc
     2581 accggcgaga ataatgcgga aatcttcttt taccatcaac gtgttagcaa cgttgagatt
     2641 gttggtgaca atgcgcaaat tgctgtgatt gagcagcgcg tgcgccaccg cttccggcgt
     2701 ggtgccgata tcgataaaca gcgtcgagcc attggggatt tgctccgcca ctttgcgggc
     2761 gatgcgctct ttttcttcgg tctgggtggc tttacgatcg tgccacggcg tgttaaccga
     2821 actggaaggc agcgccgcgc cgccatgatg gcgcaggatc aggttttgct ctgccagctc
     2881 gttgaggtcg cggcgaatag tctgcgggct gacggagaag tgctccacca gttcctcggt
     2941 actgacataa ccctgctgtt taaccagttc gataataccg ttgtgacgtt gtgtttgttt
     3001 catttataaa tccctggaat tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca
     3061 tcgctaaacc cacggctaat ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca
     3121 aatcaaacca tccggcgaca atccagatca gcgcaaagat aattaaccca cgttgcaggt
     3181 aaatgccact ttgcggatcg cgttcgccac gtagccagac gtagcccatc agcgcataca
     3241 ccacgccaga aagcccgcca aaccacggcc cgctgaattt ttgctgcaca tagccgctta
     3301 acagggcgct gataagcgta atgacaatta gcttaccgct accgaggcgt ttttccaccg
     3361 cgccgccgag ataccaccac caaagcaggt taaagaggat atgcatcagc gagaagtgca
     3421 ttaacgcgtg ggtgaagtaa cgccagaact caaatttcag tgttggatcg aatggccagg
     3481 ccagccataa catcacttcc tgatcgccga gaatttgcat ggcaataaac accaccacgc
     3541 aggcgatcat catcacccag gtcaccggac ctgcgcgttc acgcaaggca gcaaagaaag
     3601 gataacggcg ataatgcagg ccactgccgg tatggcctgc cagccagctg gccgccagat
     3661 aacgcggatc tgccgggttt tcgagaaaac gcgccagccc cgcccgcacg cgctcggcct
     3721 gggactcatc cgccagccag acatcgcttt ggttatgttg ttgaatcgtg aggataacac
     3781 cctgcgtcgc catgtaatca acaaacgcct gcgccacgcg ggggttagca aaagaggtaa
     3841 tcatcaacat cgttgctgtc gcttattcca cacaaaaggg gacagtataa agcgttacgc
     3901 gccgtacgcc acctctgcgg gaaactgtcg ctgccaggct tcaaagccgc cgtcaatgct
     3961 atagaccaca tcgtagccct gttgcagcag atactgcgcc gcccctttgc tgctattgcc
     4021 gtgataacac atcaccatca ccggagtgtc aaagtcgtta tcacgcataa acgcgcccag
     4081 cgtgtcgttg gttaaatgga aagcctgcac cgcatgcccc atagcgaaac tctgtggatc
     4141 gcgaatatcg accagcaccg cctctttttc ctgcaacttc tggtgcgcgt cggcaacgtt
     4201 aatacattcg aactgatcca tgcgtctctc tttctttaca aacaagtggg caaatttacc
     4261 gcacagttta cgtcgaagcg gcagataaac gccataatgt tatacatatc actctaaaat
     4321 gttttttcaa tgttacccaa agcgcgattc tttgctaata tgttcgataa cgaacattta
     4381 tgagctttaa cgaaagtgaa tgagggcagc atggaaacca aagatctgat tgtgataggt
     4441 ggcggcatca atggtgctgg tatcgcggca gacgccgctg gacgcggttt atccgtgctg
     4501 atgctggagg cgcaggatct cgcttgcgcg acctcttccg ccagttcaaa actcattcac
     4561 ggtggcctgc gctaccttga gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa
     4621 cgtgaagtgc tgctgaaaat ggctccgcat atcgccttcc cgatgcgttt tcgcctgcca
     4681 catcgtccgc atctgcgccc ggcgtggatg attcgcattg gtctgtttat gtacgatcat
     4741 ctgggtaaac gcaccagcct gccgggatca actggtttgc gttttggcgc aaattcagtg
     4801 ttaaaaccgg aaattaagcg cggattcgaa tattctgact gttgggtaga cgacgcccgt
     4861 ctggtactcg ccaacgccca gatggtggtg cgtaaaggcg gcgaagtgct tactcggact
     4921 cgcgccacct ctgctcgccg cgaaaacggc ctgtggattg tggaagcgga agatatcgat
     4981 accggcaaaa aatatagctg gcaagcgcgc ggcctagtta acgccaccgg cccgtgggtg
     5041 aaacagttct tcgacgaggg gatgcatctg ccttcgcctt atggcattcg cctgatcaaa
     5101 ggcagccata ttgtggtgcc gcgcgtgcat acccaaaagc aggcctacat tctgcaaaac
     5161 gaagataaac gcattgtgtt cgtgatcccg tggatggacg agttttccat catcggcacc
     5221 accgatgtgg aatacaaagg tgatccgaaa gcagtgaaga ttgaagagag tgaaatcaat
     5281 tacctgctga aagtgtataa cgcgcacttt aaaaagcagc taagccgtga cgatatcgtc
     5341 tggacctact ccggtgtgcg tccgctgtgt gatgatgagt ccgactcgcc gcaagccatt
     5401 acccgtgatt acacccttga tatccatgat gaaaacggca aagcaccgct gctgtcggta
     5461 ttcggcggta agctgaccac ctaccgaaaa ctggcagaac atgcgctgga aaaactgacg
     5521 ccgtattatc agggtattgg cccggcgtgg acgaaagaga gcgtgctgcc gggtggtgcc
     5581 attgaaggcg accgcgacga ttacgccgct cgcctgcgcc gccgttaccc gttcctgact
     5641 gaatcgctgg cacgtcacta cgcgcgcact tacggcagca acagcgagct gctgctcggc
     5701 aatgcgggaa cggtaagcga tctcggggaa gatttcggtc atgagttcta cgaagcggaa
     5761 ctgaaatacc tggtggatca cgaatgggtc cgccgcaccg acgatgccct gtggcgtcgc
     5821 acaaaacaag gcatgtggct gaatgcggat caacaatctc gtgtgagtca gtggctggtg
     5881 gaatatacgc agcagaagtt gtcgctggcg tcgtaaatta acgtaaggtg atcaggtcag
     5941 atttcaatct ggcctgagac tgatgacaaa cacaaaactg cctgatgcgc tacgcttatc
     6001 aggcctacgt ggtttatgca atatattgaa tttgcatggt cttgtaggcc agataagacg
     6061 ttcacgtcgc atccggcatg aacaaagcgc actttgtcaa aaatctaacc tactttttaa
     6121 ttcagggaat taccgcaaag cccacgccat catacccaac gtaacaaaac tcaggcacgt
     6181 tccccactcg ccccgagaaa aatagcatta atgcgcccag cgtcagcata aaaatattga
     6241 gcagaaggta aattgttggc gtaaagagta aattgcaaac ctcaggaata gcggcaagcc
     6301 cagcccccag catggcaata accgctgcaa aaaattgcgt cagtattggg atgccgaaaa
     6361 aagtcattac cagcgaggtc aaaatccatt ttctattttg cattattctt tccattcttt
     6421 ctgaatggcg aaattatact ccccgagtcc ccttgcccct tctggacact tttccgaaat
     6481 gatggcgaaa aaaacgggac cctttggc
//
LOCUS       DADLSB010000196         6494 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.196, whole genome shotgun sequence.
ACCESSION   DADLSB010000196 DADLSB010000000
VERSION     DADLSB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.196"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JN331_003711"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JN331_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY7261720.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JN331_003712"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JN331_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY7261721.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="JN331_003713"
     CDS             complement(2659..5772)
                     /locus_tag="JN331_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY7261722.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5827..6054)
                     /locus_tag="JN331_003714"
                     /pseudo
     CDS             complement(5827..6054)
                     /locus_tag="JN331_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
     gene            complement(6279..>6494)
                     /locus_tag="JN331_003715"
     CDS             complement(6279..>6494)
                     /locus_tag="JN331_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261723.1"
                     /translation="RLSSILQHEDQEAFEEIFKTSCFMPKKGLKVDKVVPMGWTSGVA
                     HVKVYVKGNLYDLWTNTENLQSEKAGK"
BASE COUNT         1722 a         1513 c         1518 g         1741 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgatctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctggcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagcaaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctag ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atgatgtgat tacaggttga ctactgcttt atttaccagc cttctcggac
     6301 tgaagatttt cggtgttagt ccaaagatcg taaagattgc ctttcacata aactttgaca
     6361 tgcgcaaccc cactggtcca ccccatagga accactttat caacttttag ccccttttta
     6421 ggcataaagc atgatgtttt aaagatctct tcaaatgctt cttgatcttc gtgttgcaaa
     6481 attgaactta atcg
//
LOCUS       DADLSB010000197         6290 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.197, whole genome shotgun sequence.
ACCESSION   DADLSB010000197 DADLSB010000000
VERSION     DADLSB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.197"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1031
                     /locus_tag="JN331_003716"
     CDS             <1..1031
                     /locus_tag="JN331_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HAY7261724.1"
                     /translation="LAAISLVLLVIIATGGDYLMAKLKGDGSGKSASHEPESHATSPA
                     RERNNLGRDLNPLGQLADTTGQEKTTPTDTAAPPQASPPPLTFDKVSALADSAPTTAS
                     SAPKSTSSSNVSPAGSASAPGSATPAGQSQDNGLARITGVKRLGLDPDLYIPADRHIP
                     CAMMQRFVSDVGGRFNCLISEDIYSASNAVRLIPAGTEARGLYRTGTLKNGQGRLFLA
                     ITELRTPEPGRLVIPMVDSQAVGALGENGVAGWIDNHWLERIGNTLLLGTVQDFAAAA
                     SGSSPGKDRNTDYTENTRAATAEMAKTLLENSINIPPTMYLNQGDVIGLVTGADIDFS
                     DVYRLRMR"
     gene            1035..2084
                     /gene="virB11"
                     /locus_tag="JN331_003717"
     CDS             1035..2084
                     /gene="virB11"
                     /locus_tag="JN331_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HAY7261725.1"
                     /translation="MDDKLTDTPSEGNVALSHLRDLLFGDYLSREGLTEIAINRPGEL
                     HTKINGAWEMHASPVTLRQCSSFARALAVWNNDTIDETSPILSATLPTGERCQIVVPP
                     ACERNTISITLRKPDYFQRTHQSYIDAGFYDRVQGTEKVESKDGELSRIYLSGNIPLF
                     MEKCVEYGKTLFVVGETGSGKTTYMKMLLHYIPETLRLITIEDNPELKFYSQKNYVHL
                     FYPADAGEGAIVTSDRLVRAIYRMNPDRPLLAEVRGREAWDALKLIESGHEGFMSSLH
                     AGSPRECISGLIDRCYEHPACRGMPVDVLLRKVLHCVDVIVSVDVHGNVRRMSDVYFK
                     PLHMQAMKESFNDAA"
     gene            2071..2472
                     /locus_tag="JN331_003718"
     CDS             2071..2472
                     /locus_tag="JN331_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261726.1"
                     /translation="MMLPKLTGLTAIVVLLAGCSSPPEPAAVDWSQPAQPVTTTRPVW
                     EPNRLIVASPVTEGHWSQVLTRFEPGGIYPAAQWYAVAHASRAVVNAPDGQRFFAAKT
                     WLREGGYKGVVEFRPRSGCLTCDTSEIVFYR"
     gene            2563..2880
                     /locus_tag="JN331_003719"
     CDS             2563..2880
                     /locus_tag="JN331_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261727.1"
                     /translation="MKHTVITAALLCALTSPAFAADGQNADPNDPCAMVLCLAGKLDG
                     SSPAECDPMYKNFMSIKKKKKGSFLPDHTAAARQKKLSACPSADSAIVGKIISKFGRM
                     NGW"
     gene            2907..3242
                     /locus_tag="JN331_003720"
     CDS             2907..3242
                     /locus_tag="JN331_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261728.1"
                     /translation="MINQTVSINNFSGLLKDEELPAGDLQTVLLVLSEISQKQDHFQA
                     NTSELVQLAAQHSPTFQALPAERQSFFRSVLSMPVFFYTVPLSDDEAAEWEKLNDKDA
                     LQWQQKPDL"
     gene            3218..3526
                     /locus_tag="JN331_003721"
     CDS             3218..3526
                     /locus_tag="JN331_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261729.1"
                     /translation="MATKTGFIAPPWPHVRGDSVSAASLISRIEKEAHRGCGLHYEIY
                     QYRALCELHDVLTSLSVRDAETFMTVAADHGFSLSDNDFQESRMMYFETMDEIRRDQE
                     "
     gene            3523..5724
                     /locus_tag="JN331_003722"
     CDS             3523..5724
                     /locus_tag="JN331_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAY7261730.1"
                     /translation="MRLWIAEKPAVASDIAKALGGNFTRHDGYAESATDIVSYCVGHV
                     LEMVPPETINPAYATWSLDTLPLKLYPVQLQPKASVAKQANTLVKLIRRPDIKMVVHC
                     GDPDDEGQLLVDEVLEFAGNTAPVKRALINDNTAPAVKKAINSLRDNSDFRGMYLKAL
                     MRSAGDAIFGFSMSRCYTLKARDKGYKGTISVGRVQTPVLGMIVRRWRDNQAHSEAFY
                     YQLAGQFISGTDVVCARWQTSEYAPVDDKKRLTDKAWGEGLARALAGKPASVLAAATD
                     RGKTTAAPLPFNLLRLQVYMNRRHKLTAQQTLDITQKLKDSKYITYNRSDCSYLSDEQ
                     FNDAPQVVAALKTLDVFNGLTTDTAQKSAAFDSKKVTAHTAIIPTTNMPDLSRLTDKE
                     KAVYLTIAQFYLAQFVAKKRYDESIAEIKCGDEMFKVSARKITDAGFTTFLNDAEEDD
                     EEDENSTSFEAISRLQTGATLTCREVVISEKKTKPLPLFTEATLLAALVRVADFVADP
                     RIKKLLKEKDKDKKDEHGGIGTPATRAAIIETLKTRDYITVEKGKFIPTAAGLALIDA
                     LPDSVTQPDMTAVWAEKQAAVETGEMTIERFIDGLYAEVSRLVESADIAISATEKHPV
                     KTALNRLAVKCPSCGSELVMTPKTCACTGCQFKIWLEVRGKKLTEKQAEKLIGKGKTD
                     VIKGFKSKKNDDTYSMIVTLKNAQTGELGFEYPPRDPLKGLNVSMPKELKL"
     gene            5721..6113
                     /locus_tag="JN331_003723"
     CDS             5721..6113
                     /locus_tag="JN331_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HAY7261731.1"
                     /translation="MNRIQDELTSERALKKFLRESDFAFLEMLSARISAALDEKRAEH
                     IEQEKLRRQREEKRNELLALIESEGFSPTELVSTTPAKKSAKRKAKYQYFENGVHKKW
                     SGVGRVPVVIQQELDAGKPLESFLITTD"
BASE COUNT         1484 a         1802 c         1816 g         1188 t
ORIGIN      
        1 tgctggccgc catcagcctg gtgttactgg tcattatcgc caccggcggg gattacctga
       61 tggcgaaact gaagggtgac ggcagcggaa aaagcgcgag ccatgaaccg gaatcgcacg
      121 ccacctcccc cgcccgcgag cgcaataacc tcggacggga tctgaacccg ctgggacagc
      181 tggcagacac aaccggccag gagaagacta ctccgactga taccgccgcg ccgccgcagg
      241 catcaccgcc ccccctgacg tttgacaagg tcagcgccct ggcagacagc gccccaacca
      301 ctgcaagcag cgccccgaaa agcacgtcca gcagtaacgt atcacctgcg ggcagcgcgt
      361 ccgcgccagg ctctgcaaca cccgccgggc agtcgcagga taacgggctg gcgcgcatta
      421 ccggcgtgaa gcggctcggc ctcgatccgg atttgtacat tccggccgac cggcatatcc
      481 cctgcgcgat gatgcagcgc tttgtatcgg acgtgggcgg gcgctttaac tgccttatca
      541 gcgaggacat ttacagcgcc agcaacgccg tgcgccttat cccggcaggc actgaggcgc
      601 gcggcctgta ccgcaccggc acgctgaaaa acgggcaggg gcgtctgttc ctcgccatca
      661 ccgagctgcg cacgccggaa cccggccgcc tggttattcc gatggttgac agccaggccg
      721 tgggggcgct tggcgagaac ggcgtcgcgg gctggataga caatcactgg ctggagcgga
      781 taggtaatac cctcctgctc ggcacggtac aggactttgc cgctgcggcc tccggctcgt
      841 cgccgggcaa ggatcgcaac acggactaca cggagaacac ccgcgcggcc acggcggaaa
      901 tggcaaaaac gctcctggaa aacagcatca acatcccgcc gacgatgtac ctcaaccagg
      961 gtgacgtgat tggcctggtc acgggggcgg acattgattt ttcagacgtc tacagactgc
     1021 gcatgaggta agcgatggac gataaactga ctgatacccc gtcagagggc aacgtcgcgc
     1081 tgtcacacct gcgcgatctg ttgttcgggg actacctgag ccgggagggg ctgacggaaa
     1141 ttgccatcaa ccgccccgga gagctgcaca caaaaatcaa cggggcgtgg gaaatgcacg
     1201 cctcgccggt cacgctgcgc cagtgctcgt cctttgccag ggcgctggcc gtgtggaaca
     1261 acgacaccat tgatgaaacg tcgcctatcc tctcggccac gctccctaca ggagaacgct
     1321 gccagatagt ggtgcccccg gcctgtgagc gtaacaccat ctcaatcacc ctgcgtaagc
     1381 cggactactt ccagcgcacc catcagtcgt atatcgatgc cgggttttat gaccgggtgc
     1441 agggtacaga aaaggtggaa tcaaaagacg gggagctgag ccgcatatac ctgtccggga
     1501 atatcccgct ttttatggaa aagtgcgtgg agtacggcaa aacgctcttt gtggtggggg
     1561 aaacaggctc tgggaaaacc acctacatga agatgctgct gcactacatt cccgagacgc
     1621 tgcgccttat caccatcgag gacaacccgg agctgaagtt ttacagccag aaaaactacg
     1681 ttcatctgtt ctacccggcg gacgccggag agggcgcaat agtcacctct gatcgcctgg
     1741 tgcgcgcgat ttaccgtatg aaccccgacc gccccctgct cgccgaggtg cgtggccgcg
     1801 aggcgtggga cgcactcaag ctgattgagt ccgggcacga ggggtttatg tcctccctgc
     1861 acgcgggcag cccgcgcgag tgtatctccg ggctgattga ccgctgctac gagcatccgg
     1921 cgtgccgggg catgccggtt gacgtgctgc tgcgcaaggt actgcactgc gtcgacgtca
     1981 ttgtgagcgt ggacgttcac ggcaacgtgc gccgcatgag cgacgtctat tttaaacccc
     2041 tgcacatgca ggctatgaag gagagtttca atgatgctgc ctaaactgac cggcctgacg
     2101 gcgatcgtcg tcctcctggc gggctgttct tcgccaccgg agcctgcggc cgttgactgg
     2161 tcacaaccgg cgcagccggt aacgaccacc cggccggtat gggaacccaa ccgtcttatc
     2221 gtggcctcgc cggtgacgga agggcactgg tcgcaggtgc ttacgcgttt tgaacccggc
     2281 gggatttacc cggccgcaca gtggtacgcg gttgcgcacg cctcgcgcgc ggtagtaaat
     2341 gccccggacg gccagcgctt ctttgcggcc aaaacctggc tccgcgaggg cggctacaag
     2401 ggtgtggtgg agtttcgccc ccgctccggc tgcctgacct gcgacacctc agaaatcgtc
     2461 ttctaccgat aacgtcggtg acggtctgag tccttcagac cgtcaccgac gttgaacaac
     2521 caacgggcgt tagcgccctg cttcccttca gaggaaatca tcatgaaaca caccgtaatc
     2581 acggcggcgc tgctgtgcgc ccttacttct ccggcgttcg ccgccgatgg ccagaatgcc
     2641 gacccgaatg acccgtgcgc gatggtgctg tgcctggccg gaaagctcga cggcagcagt
     2701 cccgccgagt gtgacccgat gtacaaaaat ttcatgagca tcaaaaagaa aaaaaagggg
     2761 tcgtttttac ccgatcacac cgccgcagcg cggcagaaaa aactgagcgc ctgcccgtcc
     2821 gctgacagcg ccattgtcgg caagatcatc tcgaaatttg gccgtatgaa cggttggtaa
     2881 aacggcagcc gtaaaggaga caccacgtga ttaatcaaac cgtttctatt aataactttt
     2941 ccgggctgct gaaagatgag gagcttcctg caggtgattt gcagacggtg ctgctcgttt
     3001 tgtcggaaat cagccagaaa caggatcatt ttcaggccaa cacgtccgaa ctcgtccagc
     3061 tcgccgcgca gcactcgccg accttccagg cattaccagc agaacgtcag tcgttcttcc
     3121 gctccgtgct cagtatgccg gtcttctttt acaccgttcc gctgagcgac gacgaggcgg
     3181 cggaatggga aaaactcaac gataaggatg cactccaatg gcaacaaaaa ccggatttat
     3241 agccccgccg tggccgcatg tgcgtggtga ttccgttagt gccgcgagcc tgatttctcg
     3301 catcgagaaa gaagcgcatc gcggctgcgg cctgcattac gaaatctacc aatatcgcgc
     3361 cctgtgcgaa ttgcacgatg ttctgaccag tctcagcgtc cgtgatgctg agacgttcat
     3421 gacagtagcg gcggatcatg gctttagtct gtctgataac gactttcagg aaagtcgaat
     3481 gatgtacttc gaaacgatgg atgaaatcag gagagatcaa gaatgagact gtggattgca
     3541 gaaaaacccg cagtcgcgag cgatatcgcg aaagcgctgg gcggcaactt cacccggcac
     3601 gacggctatg ccgaatcggc gaccgatatc gtgagctact gcgtcggcca cgtgctggaa
     3661 atggtgcccc cggagacgat caacccggcc tacgccacct ggtcgctcga caccctgccg
     3721 ctgaagctgt acccggtaca gcttcagcca aaagcgagcg tggcaaagca ggctaatacc
     3781 ctggtgaagc tgattcggcg cccggatatc aaaatggttg tccactgcgg cgacccggac
     3841 gacgaggggc agctcctggt tgacgaggtg ctggagttcg ccggaaacac cgcgccggtg
     3901 aaacgggcgc ttattaacga caacaccgcg ccagcggtga aaaaagccat caactccctg
     3961 cgcgataaca gcgattttcg tgggatgtac ctgaaagccc tgatgcgcag cgcaggagac
     4021 gcgatttttg gcttcagtat gagccgctgt tacaccctca aagcccgcga taagggctat
     4081 aagggcacga tttcggttgg ccgcgtccag acgccggtgc tgggaatgat tgtgcgccgg
     4141 tggcgcgaca atcaggcgca cagcgaggcg ttttactacc agctcgcagg gcagtttatc
     4201 agcggtacgg acgttgtctg cgcccgctgg cagacgtccg aatacgcgcc ggtggacgat
     4261 aaaaagcggc tgacggataa ggcctggggg gaaggactcg cgagggctct ggccggaaaa
     4321 ccggccagcg tgctcgcggc cgccaccgac cggggaaaaa ccacggccgc accgctgccg
     4381 tttaacctgc tgcgcctcca ggtgtatatg aaccgcaggc acaaactgac cgcgcagcag
     4441 acgctcgata tcacgcagaa gctcaaggac agtaaataca ttacctacaa ccgctctgac
     4501 tgttcttatc tgtccgatga acaattcaac gacgcgccac aggtggtggc ggccttaaaa
     4561 acgctcgacg tttttaacgg ccttaccacc gacacggcgc aaaaaagtgc ggcgtttgac
     4621 agtaaaaaag tcaccgccca caccgccatt atcccgacga cgaacatgcc ggatttaagc
     4681 cgccttaccg acaaggaaaa ggcggtttat ctcaccatcg cgcagtttta tctcgcgcag
     4741 ttcgtggcga aaaagcgcta tgacgagtcg atcgcggaga ttaaatgcgg tgacgagatg
     4801 tttaaagtct cggcgcgtaa aatcaccgat gcgggtttta cgacgttcct gaacgacgcg
     4861 gaagaagacg acgaggagga cgaaaacagc acctcgtttg aggccatcag ccgcctgcaa
     4921 accggggcga cattaacctg ccgcgaggtg gtgatttcgg agaaaaaaac taaaccgctg
     4981 ccgctgttta ccgaggcgac cctgctcgct gccctcgttc gcgtggccga ttttgtcgcc
     5041 gatccgcgca ttaaaaaatt gctcaaggag aaggataaag acaaaaaaga tgaacacggc
     5101 ggcatcggca caccggcgac ccgcgcggct attattgaga cgctgaaaac ccgcgattac
     5161 atcaccgtgg aaaagggcaa atttatccca actgccgccg ggctggcgtt aattgatgcc
     5221 ctgcccgact cggtgacgca gccggatatg accgccgtat gggctgaaaa acaggcggcg
     5281 gttgaaaccg gcgaaatgac catcgagcgc tttattgatg gtctttacgc ggaagtttcc
     5341 cgcctggttg agagcgccga tattgccatc agcgcgacgg aaaaacatcc cgttaaaacg
     5401 gcgcttaacc gcctcgcggt taaatgtccg tcgtgcggtt ctgagctggt tatgacgccg
     5461 aaaacctgcg cctgtacggg ctgtcagttc aaaatatggc tggaagtgcg cggcaaaaag
     5521 ctgacggaaa aacaggccga gaagttaatc ggcaagggca aaaccgacgt tattaaaggt
     5581 tttaaatcga agaaaaacga cgacacctat tcgatgatcg tcacgctgaa aaacgcgcaa
     5641 accggggaac tcggttttga atatccacca cgcgatccgc tgaaaggatt gaacgtatct
     5701 atgccgaagg agctgaaatt atgaaccgta tccaggacga attaacctca gagcgcgcgc
     5761 tgaaaaagtt tttacgtgaa agtgattttg cctttctgga gatgctgagt gcacgtattt
     5821 cagcagcgct tgatgaaaaa cgcgcagagc atatcgagca ggaaaagcta cgccgacagc
     5881 gggaagaaaa gcgcaatgaa ttgctggcgc tgattgaatc ggagggcttt tcaccgacag
     5941 agctggttag tacaactccc gccaaaaagt cggcaaaacg caaagctaaa tatcagtatt
     6001 ttgaaaacgg cgtgcataaa aaatggtcag gcgtcggacg cgtgccggtt gtaattcagc
     6061 aagaacttga cgccggaaaa cctcttgaat ccttccttat taccaccgac taacaacggc
     6121 acagggtcgg ttttgacctg gcttttcggg cttcgaagac tggcgagcat ggcgattcaa
     6181 ttttgaaaaa attgctaatc gacaggccgc ctgagtctgc gaagtggcag gaggcgagta
     6241 aattcaattt cgaagaaatt gctaatttgc gtcgccgatt ccactttttg
//
LOCUS       DADLSB010000198         6202 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.198, whole genome shotgun sequence.
ACCESSION   DADLSB010000198 DADLSB010000000
VERSION     DADLSB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.198"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            186..513
                     /locus_tag="JN331_003724"
                     /pseudo
     CDS             186..513
                     /locus_tag="JN331_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(543..3455)
                     /locus_tag="JN331_003725"
     CDS             complement(543..3455)
                     /locus_tag="JN331_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261732.1"
                     /translation="MFSKAFLRKISMFFARRKQAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTRLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3458..3574
                     /locus_tag="JN331_003726"
     CDS             3458..3574
                     /locus_tag="JN331_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7261733.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3938..4780
                     /locus_tag="JN331_003727"
     CDS             3938..4780
                     /locus_tag="JN331_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7261734.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAEGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4783..5871
                     /gene="capU"
                     /locus_tag="JN331_003728"
     CDS             4783..5871
                     /gene="capU"
                     /locus_tag="JN331_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY7261735.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5876..>6202
                     /gene="virK"
                     /locus_tag="JN331_003729"
     CDS             5876..>6202
                     /gene="virK"
                     /locus_tag="JN331_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7261736.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEF"
BASE COUNT         1639 a         1447 c         1484 g         1632 t
ORIGIN      
        1 ggccgccggg gacgtttttt taccaccggg agtttgggca taacggtgct ttcagtaagt
       61 acgacaggta ctgtggaggc acttccgtaa agaagggcat caagctcctg ctccgacata
      121 cctgcaggca aaggccatga aagccctgat ttccggaagc gagcgaacag tccacaaact
      181 gttgaacgtg gtgtcagcgg cggacaggga taagccctcg tcagcagttc cgtcagcact
      241 gcgacagcgt tctttacccg gtcaaaacag cgttgcggtg caccacgcct gacggatgaa
      301 ctgcgtgctc agggttacca ctttaacgta aaaaccgtgg cggcaagcct gcgccgtcag
      361 gggctgaggg caaaggcctc ccggaagttc agcacggtca gttaccgcgc acatggcctg
      421 cccgtgtcag aaaatctgct ggagcaggat ttttacgcca gtggtccgaa ccagaagtgg
      481 gcgggagaca tcacgtactt acgtacagat gaagttcggt tacatcctgt cagcacggaa
      541 cctcacgctt tttgagcggc acgacggatc ccccccaccc agtatccgac agacttttca
      601 ctgcgttcgt aaaaaccctt cttccctccc agcatatcca ctaattccgg tgacatatcc
      661 tgcagataag tctgcaaatc atgccccaga tcttccggtg actgcaactc ataaaccgcg
      721 cgccacacat caccctgcct tattttaagc cccgggttaa gaatatactg tccgtggttc
      781 agcctcagaa aaagcttttt tccataagga tttttgctgt taacttcatg ttgagagagc
      841 tggctgctga tataactcct tcgtttgcgg cgttccggca gcaacgcttc aggaagatga
      901 accacacact ctgccagtcg tgccgcactg aaggcttccg aaaaaaacag agcacttccc
      961 agatgcgtgt ggaacagcgc ctgcatcagg atcaccagaa acattgccat ctgtcggttg
     1021 tccagtttga tgaggcgtcc ttccacctgc agtgacagac tttcaggttt cagccgggta
     1081 tacaactgcg cacttttctg ttgcgcataa cggggcgtac tgaccgccgc tgacagaact
     1141 tgatgataag ccgccagccc gtcattaccg gtcagcaagg gatctgcacc acgctccagc
     1201 agtaactgaa ccagtgcaat attacctgcc cgggctgctg acatcagcgg agtaagatta
     1261 aacggcgaac ggtgatcaac accataacgt tcaatatccc gcaacacggc aaccgggtta
     1321 ttcattgagt atggacggaa atacttcagc tccagtaact gcagagtctt cgttctgggc
     1381 tgccttgtgg gcttaaaatc ttcccggtac agtgcattat acagcgggaa cagggaataa
     1441 atcagactgt actccagcgc ctgcagttgt atggttttat cggctgtacc tgcgaggata
     1501 tgctgtttcc actgtcgtgc atcctctgcg gtgataatct gccatggcat ctcagatgtc
     1561 tgcaggatcc ggctacggat ggtgtccgcc tgctcctgct tcccctgttt ttcaagcaga
     1621 tgtgcggttt tctgccactc atcacggctg gaaatctctt cctgcagatt cagaggctct
     1681 tcctgatgcg tgagcgcaag cagactccac agaggatgct ccacctgctg ttcaacaaga
     1741 tacacattgt gtgtagcacg ggtgagcgca acatacagcg cgttggtaaa aaatttatag
     1801 atttccgctg aacgatcctg cctgtcccgc ggacgggaat aacgtgcctc gccctcaaga
     1861 tcagccggtg taagaccctc gcaaatatca tcaaatgcct gccgggcagc gcttactata
     1921 ttgtaaagga tcaccgtttc gtattccagc cccttaacct cctgaaccga aaagaccagc
     1981 ggcgtactga accagcatcg cgcctgtgct ttctgttccg gatgcatcac aatgaccgct
     2041 acacgattcg aaagtgaggt tttggcattc agttcctgct ttgtctcttc cctgtcgtcc
     2101 agcaaccgga tggtcccttc tgcctgaccg cagcttctga caaaatggtg actttcccga
     2161 tcaatagcac taaagcggac ctgtttgagt ctcagcaacc ggttagccag tgcagtgacg
     2221 tggtgcccgt tacggtaatt cgcctgcagg atattaacgg ttgtgtcgtt tccctgttgc
     2281 tgccggaaaa agagactttt gagcgaactc catgagaaaa agctcggatg aactatctga
     2341 ttggcatcac cacagagtaa aaactgaccg ggatgacgta gcgttttcag taccagttgc
     2401 agttgaacag gcgtcatatc ctgaacttca tcaacaaaaa tgacatcata acggggagtt
     2461 gccaacgaaa gatactgatg actgagcaga ttactgtcat actgatgcga ctgtttcagc
     2521 catgcgatat aacgttcaaa caggacatag accgtcgggc ggtcctccat gccatagagc
     2581 gactgccgta tccccagtga cagatacgcc tcccgggaca gcggcccatt cccggaggca
     2641 accgcaccga tcacgcccct gaactcctca tacagggtat gggcagcccc cagtgcggcg
     2701 atagccctgt tgcggggcag ccagtcgctg aacgcactga aagtcacctc cctgccttcc
     2761 ggaatgcgca gagtttccag aaattccgtc agcgaaagaa agtcaatatt ctgtacctca
     2821 ctcccttccc ctgaagcgtc atagagagta cgcgcttttt ccaccagaaa tgaggaaagg
     2881 ctgagataca gtatatcacc agcagcctgc ttcatttttt ccagtaccag cgccgtctta
     2941 ccacttcccg ccgagccaac aatcaccagc ggtagcgggt gttcgtacag tgcctgctga
     3001 tcatcatcaa aggacagcat tttatccagg cggtggaaac gcccatgaga cggattgata
     3061 tacgtcagcg cttcagtcgc gatatcaaca ggatccggca ctggttgttg ttgtaagcgc
     3121 ccttcatcaa tcaccacgtt tctgcgaaga aaacgtgaag tgttataggc atgattgcga
     3181 atatattcca gaacaaggca gatagtttca ccgtggtagc gatacagcga aaacagtaat
     3241 cggtcccgga tactgagccg ggcgcggtaa agattggtgt caattttacg aacatcagcc
     3301 tgaacaaagt tatcggtggc aatagcctgt gcaaattttt tataacccgg gatcgtgtca
     3361 ggattcagag tatgataaag acagattttc atggctgcct gtttccgtcg tgcaaaaaac
     3421 atgctgattt tacgaaggaa tgccttactg aacaaccttg cccggaatac cccccgaaat
     3481 cagatggaac aatcagaaaa atgctctgca gttatggaat aaagaaggtg gggtggtacg
     3541 ccgggagggc gatccgctgg aaatggcgca gtagctgcag gcctgtcatg atgccggaat
     3601 agaagttcgt gtacaggtta atgaaagcgt caccccgtaa ataaactctg cgctgaaatt
     3661 cctttttttc attggagcac ctgtgttgtt ctgaggtgaa catatcacct ctgttcaggt
     3721 agccaaattc agtgtgccac ctcaaggttc ttagtgagtc gtttttaaaa gatatgataa
     3781 tatatcattg tttatgaatt gttgatgttt aatttagtaa attatatcaa atatgccatg
     3841 ggtttgctat gttattcatc cagtatctgg aaataaaatg caccagcttt attgggtttt
     3901 ccttttgtct ctgcttacta ggtgccagaa actttaaatg ttaaacgaag ggggcatttt
     3961 gttcaaagcc aatcatgttc cggtattaat gtatcatcat gtcagtcatt gccccggact
     4021 ggttacactt tctcccgtga cattccggaa gcaaatgaaa tggttggctg aaaacaactg
     4081 gaaaacactg tcatcagatg aactggagtt tttttatcgg ggcggaaaac tcccccggaa
     4141 aagcgttatg ttgacatttg atgatggata ccttgacaac tggtttcagg tatatccttt
     4201 attaaaagag tttaatctca aagcacatat ttttttaatt accggtttta ttgggaatgg
     4261 tccggttcgt cactctcctg gaaaggaata ttctcatcgc gattgcgaac atcaaatagc
     4321 gacagggaat gctgataatg ttatgcttcg ctggtctgaa gtaaacgaaa tgttgcaatc
     4381 tggtctggtc gaatttcatg ttcatacgca tactcataca cgctgggata aaaagttcag
     4441 ttcacgagaa gaacagtgta aacatcttcg gcaggacttg ttgagcggga gagaatattt
     4501 gaaagaaatg acaggaaaat gcagtaaaca tttgtgttgg ccggaaggtt attataacaa
     4561 ggattatatt caggtcgctg aagaacttgg attctattat ttatatacaa cagaaagaag
     4621 aatgaatgct cctgctgaag gaactacacg gataggaaga ataagcacca aagaacggga
     4681 aagttgcgca tggctgaaaa gacgactttt ttattataca acaccgtttt tttcatcgtt
     4741 gctggccttt cataaggggc caagattacc ggatgattaa taatgaatat actatttacg
     4801 gaatcatcac cgaatatagg tggtcaggaa ttacaggctg ttgctcaaat gaaggccctg
     4861 aagaaaatgg ggcattcagt tctgcttgtc tgcagggaaa acagcaaaat tgcttttgaa
     4921 gccagtaaat tgggaattga tatcacattc gcgttatttc gaaacagtct tcacatccct
     4981 actgcatgga gattactcgg aatagttcat ggttttcagc ccaatgcaat cgtttgtcac
     5041 agtgggcatg atagcaatat tgttggttta gtacggttat tcacttggaa acatccattc
     5101 agaattatca ggcaaaagac atatttgaca cgaaaaacaa aagttttttc aataaatcat
     5161 ttttgcgatg aggtgattgt tcccggaaca agtatgaaga cacatctgga gcaggaagga
     5221 tgtcgaaccc gggttactgt tgtgcctcca ggctttgatt tccagaaatt atacgttgat
     5281 tcccgaaaca gtttgcctcc aaatgttctt tcttggctgg cgtcccgaag gggatgcccc
     5341 gttattgctc aggtaggaat gttgcgcccg gaaaaagggc acgaatttat gttgaattta
     5401 ctgttccatt taaaaatgaa tggacgacag ttctgttggt tgattgtggg gtctggttcg
     5461 cctgaactgc gggagcattt acagtatcag attgacagta tgggcatgca tgatgatgtt
     5521 tttattgctg acaatgtttt tcctgccgcc cccgtatatc gggttgccag tctggtggtt
     5581 ctgccttcag aaaacgaatc gtttggtatg gtactggcag aagcatcggc attttctgtg
     5641 cctgtactgg ccagtcagat tggtggaatc cctgatgtta ttcagaacaa ccagaccggg
     5701 acattgttac cagcaggtaa taagcacgca tggatgtgcg ccctgaatga tttttttaat
     5761 gaccctgggc gtttttatca gatggctcgc caggcaaaac aggatataga agagcggttt
     5821 gatattaata aaactgcgtt aaaaatactc acattagcga agcacaagta acaatatgtt
     5881 ttctgtaagt aacttatcat ttatcggttt ccttaaaagg attgtttttt cctcagattc
     5941 actcccgggg aagtgggaac acagaaaatt ccggttcatg tacattttgc gatgtgctat
     6001 aaatccggtt gccagtattc gatattatta cgaactgcgt tccttgcagt gcattgagga
     6061 tattctggca atacaaccca cgttgccagc aagaattcat cgaccttatt tgcataaagg
     6121 ggggcgagcc tggtcccggg ggcaatatat tcttgagcat tatcgctttg tgcaaaattt
     6181 gccagaaaaa tattctgaat tt
//
LOCUS       DADLSB010000199         6178 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.199, whole genome shotgun sequence.
ACCESSION   DADLSB010000199 DADLSB010000000
VERSION     DADLSB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.199"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            9..161
                     /locus_tag="JN331_003730"
                     /pseudo
     CDS             9..161
                     /locus_tag="JN331_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1542..2489
                     /gene="icsP"
                     /locus_tag="JN331_003731"
     CDS             1542..2489
                     /gene="icsP"
                     /locus_tag="JN331_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY7261737.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQAETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3050..3422)
                     /locus_tag="JN331_003732"
                     /pseudo
     CDS             complement(3050..3422)
                     /locus_tag="JN331_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3425..3610
                     /locus_tag="JN331_003733"
                     /pseudo
     CDS             3425..3610
                     /locus_tag="JN331_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3653..3772
                     /locus_tag="JN331_003734"
                     /pseudo
     CDS             3653..3772
                     /locus_tag="JN331_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4022..4888
                     /gene="ospB"
                     /locus_tag="JN331_003735"
     CDS             4022..4888
                     /gene="ospB"
                     /locus_tag="JN331_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY7261738.1"
                     /translation="MNLDGVRPYCRIGNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5217..5957
                     /locus_tag="JN331_003736"
     CDS             5217..5957
                     /locus_tag="JN331_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7261739.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT         1941 a         1158 c         1240 g         1839 t
ORIGIN      
        1 gttcctaagt ggaatgtctc cacggagaac actttatcta tcgggaaata gtgcgggcaa
       61 cagtgtttaa ttatatcgag tgcgattaca atcgctggcg acggcaccgt tggtgtggcg
      121 gcctcagtcc ggaacaattt gaaaaccaga acctcgctta ggcctgtgtc catattacgt
      181 gggtaggctc acagagcctc acttacgact gcgtccatat tatgtgggta ggatcaataa
      241 agacgtttta tatgtgtctg cttgcagcca gaaaatttat accctgcagg tatcataaac
      301 atgcttgata acttaattgg ggctccctct tccttttatg gtgtaattgt ttgattgaat
      361 acttccgggg atttcagtat gaaatgaagt atatttaata tactttacaa aaagtttgta
      421 ttagtggaat aaggacaata gcttatccaa actgccaagc ctctttatta taagtaagat
      481 ctggcactgg aatattctca aaaggactaa catcatcacc acgaccttca accaaccatt
      541 ttattgcatt taaatcccag aatagttcag tagtggtcaa tttgggaaac gcacaatatt
      601 gtacggttac gttatcgcaa tagagtgaac tcccgcccta tacggcgttt tcaaggatta
      661 gtccttaatc ggacaaccac attaaagcat aataaataat taagctttaa taatatacta
      721 tagatacagt tctattataa aaaatactat tttaggtgat gcattaaatt agtagaatat
      781 ttattctttt ataaaagcac caatgaattg tccaagtaaa catgaggtaa aatgtgtaga
      841 ggataactga gtttggtcac tttaactgta ttagtcgcag tctgactctc gactttaaaa
      901 ggatgggaat tacctgtact gatagtgaga aagctttcca ttgaaataag tcaaataaaa
      961 gttgatggct tcagtgctaa agtgaggtgc ccgctagccg tattcactcg aatcggaatt
     1021 cgatgtattg cttctgccat ttccataata tttcacggga aaaacaaaat ctgaaaaact
     1081 atacttaagt gttgtattga gtcagttgag atacaaatac atatttatca gcacacgtta
     1141 cgtcaaatac aggaggttgt catgttggta ctgaaaggca cgttggctat tgcagccgtg
     1201 atgacaattt attgtcttat cgttgttctt atggaacgct tttctgattg atttcatatt
     1261 ggcgagagaa caagaaggtg agtagaattg tcgcggaatc ctgaaactat cagcctcagg
     1321 tatgaacacc aaaaggtagg tagagaaatc tatggccccc gttactttat tcgcacaatg
     1381 aggtattttt ttatttttac aaaagttcaa taaatattat aataaaagat atgttcttgg
     1441 taacataaac aaatatttaa ttaaaataat gcttggcatt gacgtttatc ttataaagta
     1501 agaagatcat ggacatttca accaaaaaag tagagttctc gatgaaatta aaattccttg
     1561 tacttgcact ttgtgtacct gcgatcttta ctacacatgc taccactaac tatccacttt
     1621 tcataccgga caacatcagt acggatatta gtctaggatc tctgagtggc aaaacaaaag
     1681 aacgcgttta tcatcccaag gaagggggga gaaaaattag tcaactggac tggaaataca
     1741 gtaatgcgac tattgttaga ggtggcatcg attggaagct aattccaaaa gtgtctttcg
     1801 gagtttccgg ttggactact ttaggtaacc agaaagcaag catggttgat aaagactgga
     1861 acaattccaa tacccctcag gtatggaccg accagagctg gcatcccaat acgcatctcc
     1921 gtgatgctaa cgaattcgag ctgaatctta aaggttggtt attaaataat ttggattatc
     1981 gacttggact aatagcaggt taccaagaga gtcgttacag ttttaatgca atgggaggga
     2041 gttatattta tagtgaaaac ggggggaaca ggaataaaaa aggggcacat cctagtggtg
     2101 aaagaacaat aggttacaaa cagctcttca aaatacctta tattggatta actgctaatt
     2161 accgccatga gaattttgag tttggagcag aactgaaata tagtggttgg gttcgttcat
     2221 ctgatacaga caaacactat caggctgaga caatttttaa agatgaaata aaaaaccaaa
     2281 attactgctc tgttgctgcg aatattggat actatgtcac ccccagtgca aaattttata
     2341 tagaaggctc cagaaattac atttctaata aaaaaggtga tacatctctt tatgagcaaa
     2401 gtaccaatat atctggcacc attaaaaata gtgcaagtat tgaatatatt ggttttctca
     2461 cttccgcagg tataaagtat attttttgaa ttggttcacc gagttgatta cgttttcatg
     2521 gctaaaaatt gccgcccatt gtgagtcata ttcgccctga ctttccagaa ccatacgaac
     2581 tgcccgttga cggacttcgg gggaaaaacg tgtattttta gtcattctgt ttacctcttt
     2641 ctcagggcgt ttagtctcca ggatttccgg ggcggttcaa aaaactcttc gttgattact
     2701 gtggacttac tgttggcatt accgaccctg agaccggaga aataagaacc gcttagatca
     2761 tcgtggctgt tctcggggta tcaagttaca catgggcaga gcctacccaa ggtctactat
     2821 tatcgcctga aagcgctgat ggaacagtgc tatcagggac atgctgatga aaatacctaa
     2881 ataatcagcc agcactctat ctttccaaat ccacagcata gcaaagagag caaaagagcc
     2941 tgtaaattca gaaattaagg aaagcctctt ggtgcacata atctatctta tgttaaatga
     3001 aatctaattt cttgattata tgctggatcg ttcgtgggtg gctttgcccc tatatttcca
     3061 gacacctgtt atcattggcc cgctgccgca gatattcccg tggcgagcga taatccagcg
     3121 cgctatgcgg atgccattcg ttataatgct cgaatgcctc tgcaaggttc tttgctgccg
     3181 ttaacccgtc aggtttgggc atgatactga tgtagtcaca ctttattgtt ttcacgaagc
     3241 tctttgctat tctgttactc tccggactcc gcaccgccgt gttcttcggt tcaacaaagt
     3301 cccaacatcc gggtgaactg gcgtgtttca ttagcccggt agcatgaacc attatccgtc
     3361 agtcacttca ctggagacgc cggaagctcg ttgccgaagc ggtgttccac cgctcccagc
     3421 atgtcgccaa cgggagagca tctacggtct gattgaactg tttattcaaa ttgtccaccg
     3481 tctgtcagta cgctctgaac gtcggctggt taaaacactg ctcgctgatt ttcagagggt
     3541 tcacggtaaa acagcacttt tattccggat tgcggaagcg gctctgaata atccggatgg
     3601 gctggttaaa atctgcatat tgttgaacca gatcgcagcg gtaagagaag ttcatcatac
     3661 ttggcgcagt tacgcgatgt ttctgctaga ggtgatgccg taaagaacgg cagaacatta
     3721 ccggaacaag atagcggtct acctgcactg gtatcagacc cggggcttcc ccggatgatc
     3781 tccacggtac tttaggtact gtgtctatat tatgtaggta ggatcaatta actttgtagt
     3841 tgaaattctt tcattgaaat aatgaataaa aaagacaaac cgcattaaac aatgccagaa
     3901 tataattgca tttttgttcc gtttttttaa atcatagtat acaaagaagc ttgcatacta
     3961 tatgaaaata taacatcctt tactatgatg tttataaaac aatatatgga gtcatgtagg
     4021 tatgaattta gatggtgtta gaccatactg tagaataggc aataaaaaga atgaaagcat
     4081 atcagatatt gcatttgcac atataataaa aagggtaaaa aattcatcat gtactcaccc
     4141 aaaagcagca ttggtttttt taggagagaa aggtttttgt gatagcaatg atgttctatc
     4201 tataatggga caacaaatac caagagtatt taagaacaag atgttatatg attatgtttt
     4261 taaaaatgaa aaaagtaaaa atgattttct aaaaatggct gaatcatggc taccacagag
     4321 tgaaccaata gtaataaata atgatgatga cgcattgaat gctgctgctt atttttctgt
     4381 aaaaaaagcg aaaataaaaa cagtaaacga tactgatttt aaagagtata ataaggttta
     4441 tattcttggg cacggtagtc ctggttctca tcaattaggc cttggttcgg aacttattga
     4501 tgtacaaaca atcatttcaa gaatgaaaga ctgtggtatt ctaaatgtga aagatatccg
     4561 ttttacttca tgcggctccg ctgataaagt ggctcctaaa aattttaaca atgcccctgc
     4621 tgaaagtctt tcttgtatcc ttaactctct gccttttttt aaggaaaaag aatctttgct
     4681 agagcagata aaaaaacacc ttgaaaacga tgagtcattg agtgatggtc taaaaatatc
     4741 cggctatcat ggatatggag ttcactatgg tcaagagctt tttccctact cacattatcg
     4801 ttcaacttca attcctgctg atccggagca tacagtaaaa agaagctctc agaaaaagac
     4861 ttttattatt aataaagaac tggattagta taaaattttt aacctatagt taattgtttg
     4921 catttaagat gaagttattt tacctttagt agaaaagtgc ttccctatga gggaaaatca
     4981 caaatcatca taatacagag acaaaacgat acgaacaaat agataaaaaa catcattctt
     5041 ttaccacatt atttttcgtg aatatgaaaa ataatgttat tactttaata taagactatt
     5101 ttttgttttt ccatcactct gttcaaattt ttccgcatga cttgtatttt ttgtaacaca
     5161 gctcgttttt tacagctgac caaaatcatc aattaattat gctaaggaaa taaattatga
     5221 aaaccaaaaa ctttcttctt ttttgtattg ctacaaatat gatttttatc ccctcagcaa
     5281 atgctctgaa ggcagaaggt tttctcactc aacaaacttc accagacagt ttgtcaatac
     5341 ttccgccgcc tccggcagag gattcagtag tatttcaggc tgacaaagct cattatgaat
     5401 tcggccgctc gctccgggat gctaatcgtg tacgtctcgc tagcgaagat gcatactacg
     5461 agaattttgg tcttgcattt tcagatgctt atggcatgga tatttcaagg gaaaataccc
     5521 caatcttata tcagttgtta acacaagtac tacaggatag ccatgattac gccgtgcgta
     5581 acgccaaaga atattataaa agagttcgtc cattcgttat ttataaagac gcaacctgta
     5641 cacctgataa agatgagaaa atggctatca ctggctctta tccctctggt catgcatcct
     5701 ttggttgggc agtagcactg atacttgcgg agattaatcc tcaacgtaaa gcggaaatac
     5761 ttcgacgtgg atatgagttt ggagaaagtc gggtcatctg cggtgcgcat tggcaaagcg
     5821 atgtagaggc tgggcgttta atgggagcat cggttgttgc agtacttcat aatacacctg
     5881 aatttaccaa aagccttagc gaagccaaaa aagagtttga agaattaaat actcctacca
     5941 atgaactgac cccataaagc tggacagcct gtatcaggct atggagggcc catagacaaa
     6001 tctaccctat atgagcatag gaggagtcta tgggcacacc acgttttacc cttgaattta
     6061 agggattact ggaaaggctg ggacatatcc ctccggcaga agcagaaaaa gcttattatg
     6121 ctgccatcgg aaacgatgat ctggcaacct gagttcacag ataaaacatt ctctagga
//
LOCUS       DADLSB010000200         6072 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.200, whole genome shotgun sequence.
ACCESSION   DADLSB010000200 DADLSB010000000
VERSION     DADLSB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.200"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..569
                     /gene="yneK"
                     /locus_tag="JN331_003737"
     CDS             <1..569
                     /gene="yneK"
                     /locus_tag="JN331_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7261740.1"
                     /translation="HHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQESPHFQDLFL
                     FFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHE
                     DLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            719..1909
                     /gene="ydeA"
                     /locus_tag="JN331_003738"
     CDS             719..1909
                     /gene="ydeA"
                     /locus_tag="JN331_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY7261741.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQAQ"
     gene            complement(1934..2599)
                     /gene="marC"
                     /locus_tag="JN331_003739"
     CDS             complement(1934..2599)
                     /gene="marC"
                     /locus_tag="JN331_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY7261742.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2811..3245
                     /gene="marR"
                     /locus_tag="JN331_003740"
     CDS             2811..3245
                     /gene="marR"
                     /locus_tag="JN331_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY7261743.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3265..3648
                     /gene="marA"
                     /locus_tag="JN331_003741"
     CDS             3265..3648
                     /gene="marA"
                     /locus_tag="JN331_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY7261744.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3680..3898
                     /gene="marB"
                     /locus_tag="JN331_003742"
     CDS             3680..3898
                     /gene="marB"
                     /locus_tag="JN331_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY7261745.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(3929..4756)
                     /locus_tag="JN331_003743"
     CDS             complement(3929..4756)
                     /locus_tag="JN331_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY7261746.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATLVPVVGLASAALLLDERLTGLQFLGAVLIMTGLYINVFGLRWRKAVKVGS"
     gene            4951..>6072
                     /gene="ydeE"
                     /locus_tag="JN331_003744"
     CDS             4951..>6072
                     /gene="ydeE"
                     /locus_tag="JN331_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY7261747.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNNLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVII"
BASE COUNT         1545 a         1530 c         1396 g         1601 t
ORIGIN      
        1 atcaccatac cgctgcagat tacttacgcc agtttgattt gcttgtcctg aattttatct
       61 ctcgcggtaa ttttgtcata ctcccccgat tatggaataa ctctgaggtt cacagatggt
      121 tcgtcaataa agatcctaac ttgatcaccg ccattctcga cataacggac agcgaattaa
      181 aagaggactt attgcaaagc ctgatggatt cattaggttc taacaaacat gtacaacccg
      241 aagtctgcat ctgcttctta tcccttttag cagaacaaga atctcctcat tttcaggact
      301 tgtttttatt tttcgccaat atgttattgc actatcacca atttatgaat cccaatgaaa
      361 gtgatctgaa tgacgtgttg atgccagcat cattaagtga tgataaaatt atcaaacata
      421 tggcgcgcag gaccctcaaa ctgtttgtaa aaaatgaaac gccgccaaaa gttactcacg
      481 aagacctggt gaaaaacagg cctcgctccc ctgtcagacc acctataccc gcaaccgcca
      541 aaacgccaga cctccctgaa cgtcattaaa ccgtgatgtt accgactctc tgacgcggaa
      601 cagaatcagc gtcagagaaa cggaaaacgc gatccagatc acaaatgcat tgtattcaca
      661 tcattaaccg ttttaagatc atttcatcac tttttcgcaa ctcacccgat aatctgttat
      721 gacaacaaac actgtttccc gcaaagtggc gtggctacgg gtcgttacgc tggcagtcgc
      781 cgccttcatc ttcaacacca ccgaatttgt ccctgttggc ctgctctctg acattgcgca
      841 aagttttcac atgcaaaccg ctcaggtcgg catcatgttg accatttacg catgggtagt
      901 agcgctaatg tcattgcctt ttatgttaat gaccagtcag gttgaacggc gcaaattact
      961 gatctgcctg tttgtggtgt ttattgccag ccacgtactg tcgtttttat cgtggagctt
     1021 taccgttctg gtgatcagtc gcattggtgt ggcttttgca catgcgattt tctggtcgat
     1081 tacggcgtct ctggcgatcc gtatggctcc ggccgggaag cgggcacagg ccttgagttt
     1141 aattgccacc ggtacagcac tggcgatggt cttaggttta ccgctcgggc gcattgtggg
     1201 gcagtatttc ggctggcgaa tgaccttctt cgcgattggt attggggcgc ttgttaccct
     1261 tttgtgcctg attaagttac ttcccttact gcctagtgag cattccggtt cattgaaaag
     1321 cctcccgcta ttgttccgcc gcccggcatt gatgagcatt tatttgttaa ctgtggtggt
     1381 tgtcaccgcc cattacacgg catacagcta tatcgagcct tttgtgcaaa acattgcggg
     1441 attcagcgcc aactttgcca cggcattact gttattactc ggtggtgcgg gcattattgg
     1501 cagcgtgatt ttcggtaaac tgggtaatca atatgcgtct gcgttggtaa gtacggcgat
     1561 tgcgctgttg ctggtgtgtc tggcactgct gctacctgcg gcgaacagtg aaatacacct
     1621 cggggtgctg agtattttct gggggatcgc gatgatgatc atcgggcttg gtatgcaggt
     1681 taaagtgctg gcgctggcac cagatgctac cgacgtcgcg atggcgctat tctccggcat
     1741 atttaatatt ggaatcgggg cgggtgcgtt ggtaggtaat caggtgagtt tgcactggtc
     1801 aatgtcgatg attggttatg tgggcgcggt gcctgctttt gccgcgttaa tatggtcaat
     1861 cattatattt cgccgctggc cagtgacact cgaagaacag gcgcaatagt tgaaaggccc
     1921 attcgggcct tttttaatgg tacgttttaa tgatttccaa gatgccgtta ataataaact
     1981 gcacacccat acataccagc aggaacccca tcaggcggga gatcgcttca atgccaccct
     2041 tgcccaccag ccgcataatt gcgccggagc tgcgtaggct tccccacaaa ataaccgcca
     2101 ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca atcagcgaag gttgaactct
     2161 gacgcactgt ggacgccgag ctaataatca tcgctatggt tcccggaccg gcggtacttg
     2221 gcattgccag tggtacaaag gcaatattgg cactgggttc atcttccagc tcttccgact
     2281 tgcttttcgc ctccggtgaa tcaatcgctt tctgttgcgg aaaaagcatc cgaaaaccga
     2341 taaacgcgac gattaagccg cctgcaattc gcagaccggg aatcgaaatg ccaaatgtat
     2401 ccatcaccag ttgcccagcg taatacgcca ccatcatgat ggcaaatacg tacaccgagg
     2461 ccatcaacga ctgacgatta cgttcagcac tgttcatgtt gcctgccagg ccaagaaata
     2521 gcgcgacagt tgttaatggg ttagctaacg gcagcaacac caccagcccc aggccaattg
     2581 ctttaaacaa atctaacatt ggtggttgtt atcctgtgta tctgggttat cagcgaaaag
     2641 tataaggggt aaacaaggat aaagtgtcac tctttagcta accttgcatc gcattgacca
     2701 aaacttgaac cgatttagca aaacgtggca tcggtcaatt cattcatttg acttatactt
     2761 gcctgggcaa tattatcccc tgcaactaat tacttgccag ggcaactaat gtgaaaagta
     2821 ccagcgatct gttcaatgaa attattccat tgggtcgctt aatccatatg gttaatcaga
     2881 agaaagatcg tctgcttaac gagtatctgt ctccgctgga tattaccgcg gcacagttta
     2941 aggtgctctg ctctatccgc tgcgcggcgt gtattactcc ggttgaactg aaaaaagtgt
     3001 tgtcggtcga cctgggagca ctgacccgta tgctggatcg cctggtctgt aaaggctggg
     3061 ttgaaaggtt gccgaacccg aatgataagc gcggcgtact ggtaaaactt accaccagcg
     3121 gcgcggcaat atgcgaacaa tgccatcaat tagttggcca ggacctgcat caagaattaa
     3181 caaaaaacct gacggcggac gaagttgcaa cacttgagca tttgcttaag aaagtcctgc
     3241 cgtaaacaaa aaagaggtat gacgatgtcc agacgcaata ctgacgctat taccattcat
     3301 agcattttgg actggatcga ggacaacctg gaatcgccac tgtcactgga gaaagtgtca
     3361 gagcgttcgg gttactccaa atggcacctg caacggatgt ttaaaaaaga aaccggtcat
     3421 tcattaggcc aatacatccg cagccgtaag atgacggaaa tcgcgcaaaa gctgaaggaa
     3481 agtaacgagc cgatactcta tctggcagaa cgatatggct tcgagtcgca acaaactctg
     3541 acccgaacct tcaaaaatta ctttgatgtt ccgccgcata aataccggat gaccaatatg
     3601 cagggcgaat cgcgcttttt acatccatta aatcattaca acagctagtt gataacgtga
     3661 caacgtcact gaggcaatca tgaaaccact ttcatccgca atagcagctg cgcttattct
     3721 cttttccgcg cagggcgttg cggaacaaac cacgcagcca gttgttactt cttgtgccaa
     3781 tgtcgtggtt gttcccccat cgcaggaaca accaccgttt gatttaaatc acatgggtac
     3841 tggcagtgat aagtcggatg cgctcggcgt gccctattat aatcaacacg ctatgtagtt
     3901 tgttctggcc ccgacatctc ggggcttatt aacttcccac ctttaccgct ttacgccacc
     3961 gcaagccaaa tacattgata tacagcccgg tcataatgag caccgcacct aaaaattgca
     4021 gacccgttaa gcgttcatcc aacaatagtg ccgcacttgc cagtccgact acgggcacca
     4081 gtgtcgccac aaacgccaga tacatcagcg acaagatggt ggtcatatca atagtaacca
     4141 gactgtgaat catggatgcg gaaccatcga gaatcagcga ggcaacaaag aagggaatga
     4201 ttgggattaa agcgctccag attaccagcg acatcaccgc cggacgcgtt gagtgcgaca
     4261 tgatcttttt attgaagatg ttgccacacg cccaactaaa tgctgcagcc agggtcaaca
     4321 taaagccgag catcgccaca tgctgaccgt tcagactatc ttcgattaac accagtacgc
     4381 caaaaatcgc taaggcgatc cccgccaatt gtttgccatg cagtcgctcc ccgaaagtaa
     4441 acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa gccagtccag
     4501 caggcatacc aaagttaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa ctgatggtta
     4561 atccataccc caagagcaaa ttcagtggta ctttcggtcg tgcaacaaaa aagatggccg
     4621 gaaaagcgac cagcataaag cgcaaaccgg ccagcatcag cggtggcatg ttatgaagcc
     4681 ccactttgat gaccacaaaa tttagccccc atacgaccac taccagtagc gccaacaccc
     4741 catcttttcg cgacattcta ccgcctctga atttcatctt ttgtaagcaa tcaacttagc
     4801 tgaatttact tttctttaac agttgattcg ttagtcgccg gttacgacgg cattaatgcg
     4861 caaataagtc gctatacttc ggatttttgc catgctattt ctttacatct ctaaaataaa
     4921 acataacgaa acgcactgcc ggacagacaa atgaacttat ccctacgacg ctctaccagc
     4981 gcccttcttg cctcgtcgtt gttattaacc atcggacgcg gcgctacgct gccatttatg
     5041 accatttact tgagtcgcca gtacagcctg agtgtcgatc taatcggtta tgcgatgaca
     5101 attgcgctca ctattggcgt cgtttttagc ctcggttttg gtatcctggc ggataagttc
     5161 gacaagaaac gctatatgtt actggcaatt accgccttcg ccagcggttt tattgccatt
     5221 cctttagtga ataacgtgac gctggttgtg ctcttttttg ccctcattaa ctgcgcctat
     5281 tctgtttttg ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc caccagcaaa
     5341 acgaaaatct tctcaatcaa ctacaccatg ctaaacattg gctggaccat cggtccgccg
     5401 ctcggcacgc tgttggtgat gcagagcatc aatctgccct tttggctggc agctatctgt
     5461 tccgcgtttc ccatgctttt cattcaaata tgggtaaagc gcagcgagaa aatcatcgcc
     5521 acggaaacag gcagtgtctg gtcgccgaaa gttttattac aagataaagc actgttgtgg
     5581 tttacctgct ctggttttct ggcttctttt gtaagcggcg catttgcttc atgcatttca
     5641 caatatgtga tggtgattgc tgatggggat tttgccgaaa aggtggtcgc ggttgttctt
     5701 ccggtgaatg ctgccatggt ggttacgttg caatattccg tgggccgccg acttaaccca
     5761 gctaacatcc gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg actggtcggt
     5821 tttatttttt ccggcaacaa cctgctattg tggggtatgt cagctgcggt atttactgtc
     5881 ggtgaaatca tttatgcgcc gggcgagtat atgttgattg accatattgc gccgccagga
     5941 atgaaagcca gctatttttc cgcccagtct ttaggctggc ttggtgccgc gattaaccca
     6001 ttagtgagtg gcgtagtgct aaccagcctg ccgccttcct cgctgtttgt catcttagcg
     6061 ttggtgatca tt
//
LOCUS       DADLSB010000201         6061 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.201, whole genome shotgun sequence.
ACCESSION   DADLSB010000201 DADLSB010000000
VERSION     DADLSB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.201"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..757
                     /gene="yegD"
                     /locus_tag="JN331_003745"
     CDS             <1..757
                     /gene="yegD"
                     /locus_tag="JN331_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7261748.1"
                     /translation="DYEATLQEEKRVLVVDIGGGTTDCSLLLMGPQWRSRLDREASLL
                     GHSGCRIGGNDLDIALAFKNLMPLLGMGGETEKGIALPILPWWNAVAINDVPAQSDFY
                     SSANGRLLNDLVRDAREPEKVALLQKVWRQRLSYRLVRSAEECKIALSSVAETRASLP
                     FISNELATLISQRGLESALSQPLARILEQVQLALDNAQEKPDVIYLTGGSARSPLIKK
                     ALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR"
     gene            complement(769..2709)
                     /gene="yegI"
                     /locus_tag="JN331_003746"
     CDS             complement(769..2709)
                     /gene="yegI"
                     /locus_tag="JN331_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HAY7261749.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALWSFGIETAAGVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(2706..3467)
                     /locus_tag="JN331_003747"
     CDS             complement(2706..3467)
                     /locus_tag="JN331_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAY7261750.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLTLNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(3464..4123)
                     /locus_tag="JN331_003748"
                     /pseudo
     CDS             complement(3464..4123)
                     /locus_tag="JN331_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            complement(4328..4747)
                     /locus_tag="JN331_003749"
                     /pseudo
     CDS             complement(4328..4747)
                     /locus_tag="JN331_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5065..5706
                     /gene="udk"
                     /locus_tag="JN331_003750"
     CDS             5065..5706
                     /gene="udk"
                     /locus_tag="JN331_003750"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY7261751.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5798..>6061
                     /locus_tag="JN331_003751"
     CDS             5798..>6061
                     /locus_tag="JN331_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY7261752.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGEL"
BASE COUNT         1555 a         1578 c         1541 g         1387 t
ORIGIN      
        1 ggattacgaa gccaccttgc aggaagaaaa acgggtgctg gtggtggata tcggcggtgg
       61 tacgactgac tgttcattgc tgctgatggg gccgcagtgg cgttcgcgtc tcgatcgtga
      121 agccagtctg ctgggtcaca gtggttgtcg tattggcggt aacgatctgg atatcgccct
      181 ggcgtttaaa aacctgatgc cactgctggg tatgggtggc gaaaccgaaa aaggcatcgc
      241 cctgccgatc ctgccgtggt ggaatgcggt tgccatcaac gacgttcctg cacagagtga
      301 tttctacagt agtgccaacg gtcgtctgct taacgatctg gtacgcgatg cccgcgaacc
      361 ggaaaaagtg gccctgttac agaaagtctg gcgtcagcgt ttaagctatc gcctggtgcg
      421 tagcgcagaa gagtgcaaaa ttgctctttc aagcgtagcg gaaacccgcg cctcactgcc
      481 gtttatcagc aatgaactgg ctacgctgat tagccagcga gggctggaaa gcgccctcag
      541 ccagccactg gcgcggattc tggaacaggt gcaactggcc ctggataacg cccaggaaaa
      601 accggacgtt atctacctga ccggcggtag cgcccgttct ccgctgatta aaaaagcgct
      661 ggcagaacag ttgccgggca ttccgattgc aggcggcgat gactttggct ccgtcaccgc
      721 cgggctggca cgctgggcgg aagtggtatt tcgttaaaag aaaatggctc agcagcggct
      781 taaatcagcc tgcgcctgag ccagtttttc tgccgcctga cgtaacggtt ccatcaacgg
      841 catggtccgt gctggagcat gaaggcggaa tcgctgcaac tctgtcgcgc cgacagtcaa
      901 cgtcgattcc agccgatgtc gtttggcggc cattttcgcc attaccgcct gtctgtctgc
      961 tggcgttacc gcttcgttcg gcctgaacac aaaacggcgt tcacagctcg ctttccagtc
     1021 gatgaccgcc tgggtcagat gatcaccaaa ccctttaact tgcttaacgc cacgacgggt
     1081 aacacctgct gctgtttcaa taccaaaaga ccataacgcc gctttacgcg cagggccaac
     1141 gccgggaata gaggcaacat caataaaaaa tccctccaga aacttctgct tctgccgttc
     1201 ccttgcagtg tcatgaagtg ctgccagagc gagtttctct tcttccggta acccgagaat
     1261 ttcgtccttc attttttcga gcatcgtccg tttggcgata aaaccttcca ggccgcctac
     1321 ctgctggatc tggcggacta aatggtcata gtccattttc gcacgattaa atgcctcacg
     1381 gcgttgctga acttctgctt tgtacgcttt gcttgtcaga ctgccaataa tccagatagc
     1441 cgccagcaca ggaaccaaaa taatataacg cggttctgcc tgaaggccgc aaagcagcag
     1501 cgataatgct gacagtgcga tctcaagcag aatgatgtat tcgcgccgta acaggcctaa
     1561 aggaagcggc ctgccagtcg gttggaaatg atcgggtaat ggcagttgca atgctggcgg
     1621 tgctactgac gccatcacca tcgcccagac ttttgccagc acaaaatccc cgccggtggt
     1681 aatgacctct tcgccgagat caataaaata gataacgcct tgattatcca gcgtacacca
     1741 cgggcagtcg gtcaaatgag cgggataaac atgcattgcc gaaacgggac atttctttaa
     1801 ctgttggcgc agagaatcaa gtgccgctac ccacgcttta gccgtcggac gcccggttgc
     1861 cacgccactt tccgtgaatg cctgctgaaa catggcttca acatcactcg gtaacatcga
     1921 tagcggaatc gatcgtggcg gcggttttaa accacgtcgc tgattatctg acgcgtaggc
     1981 ataacggaaa tgggtaatat ccgtctccag cgcattaccc gcatcagaga taagcggcac
     2041 accagaataa ggatgccggc caccaaacaa gacgtgaaaa atcagcaacg caaggccaaa
     2101 attatcgtga ttttctgtgc gttcaaagcc gacaaatgac gacaaggttt gtagctctgg
     2161 cggcgtaaaa tgcgacacgc cgacttcgca taaatgcagt gtgccattgg cgttaatctg
     2221 aaaggagtcg ctatcaatca acaccacttt gctgtcgcga cctaccataa aactgttctg
     2281 gtttacgtca cccacgacat gcccgtgctc gtgaaccgta gcaaaagatg aagcaatatt
     2341 gcgcgcaaca tagagtagaa aatcccacgc acaatgaggg taacgttgac gacgatgtgc
     2401 cgggctatag atcatatgaa tcggttcttt accagaaact tttggcatca taaaaccgat
     2461 aacttttccg cctcgtccac cgtgaagcgt tgcctgcggc caggcgacat aattcaacaa
     2521 ttgcgcgtca gctgtcgcag ccataaaggc aagtttgtcc tgttttaagg cgggtggcgg
     2581 cgtgtgataa atcttggcga cgctatcgac aaactcattg atatcataaa ccgcgccttc
     2641 accgcctttg cccagttcac ggcctggcgt aacgcactca ccagtagcag tatataaagt
     2701 gggtttcatg gtaaccacag cgccagtgcc aacgttttat catcatccgt acgctcgttt
     2761 accgccgggc tggacaaaaa ctgctttaac aattcaggta ataaatcgag ttgttcctgc
     2821 gttgctgacg ccagtccatt gaaaaacggg gtaaagaaag gcacatgcgg agaattatcc
     2881 aacatattta acgtcagtcg ctggatacca tccgtaaatg ctgcaacttt atgcacacgc
     2941 tcagtgctgg tgaaagtttc cagtctggaa acggcatctt catcggtaat gaagtgcgtc
     3001 atattggcat attcacccac catcggcgta agcggcaatt gcagaccgtg accaaaatcc
     3061 accaccacgc cgccatcgcc aatctgcata atcaaggtgc cattagccga tgaaatcaat
     3121 cccagaaacg tgcaggcaaa atcgcgaact gctaattctt tggcttccgc ctcggcaaaa
     3181 agtcgctgac gaacggtcag cacaatatcc gtggcgagga tatcattaag ccccaattcc
     3241 ccaccctgca ctttttgcga catataagcc atcgcttcat tgacggcgag catcgcgcct
     3301 tcgcccccct gcgaaacgct acctgcgcca tcagcaagga acatcaccag taatggctgc
     3361 tggtcattta gccaggcaac ctgcatctgg caggcatcct ggcaaggcaa gtccgcgctg
     3421 atgtgcgacg ttccaacagc ggatgcataa accagacgcc agctcacact gacgtccagc
     3481 cttttggcgc ttccagcaca acttccgttc ccggcgtgga gcgggaaacc gaacgcagcg
     3541 aactggataa tcagctaaac agttcacgga attgtaatcc ttgcagaggc aaaggctgac
     3601 gaacgctgat ttgtgccaga gtcttcatat ccgccccctg aacaccgatg gaaaagaaag
     3661 caaacctttt atcttcttcc ccccgaaaca ctttgttggc tgcagcctgc cactcatcgg
     3721 ttggtgcacc atcggtaatc aggaaaatcc acgggcgata atacgaaata ccatttgcac
     3781 gatactcacg tttccgctct tctaccatat ccagggcttt ggtaatggcc gcacccattg
     3841 gtgtatcgcc ctgggcaaac aggatgggcg gaaaaaaatt agcggcgctg gtaaagggtt
     3901 gttccacatg caccgggccg aacgtcacaa tgccgagttc cactcttttt aaagccagcg
     3961 aatcggcaag cagctcatcg cgaaaggtaa ccaatccggt gttaagttca ttaatagttc
     4021 tgccactcat cgagccagaa acatccagca gcagaatgca ggggcaacgt ggttccgggt
     4081 tactggcaaa atcgctggtg gcaaacgtga tttgttcgct cataagaaat cctttcttag
     4141 agaattattg aggtccggta tcataccgac tgcaataatg aaataagagc aaacgaagaa
     4201 actttcagaa ttagtcccgg cggcgcagaa aattttgcta ttcgcctcgc aagaacggag
     4261 agccgtcgat tgacactaag ggcggagtga cataatttca ggagtgaggg ttagggagag
     4321 gtttcccccc ccccaacaga ggcggaacaa tggcgctgcc gaatgccaga cgcagccagt
     4381 cagccagatt ttgcaagggg ttataccacg gcagcaattt acgtagcagc actgccccga
     4441 ccacggcttc aacaatattg atggtcgtcc aggtcatgtt cagcgagctg gtggaaaaaa
     4501 gcaggatgga tgcggcgata tttcccagcg aacagctcag cgcaattccc ggcaacatgc
     4561 gcccggcatg gcgataaaac gccaccatca tgatggacgt cgggaaccac agtggggcga
     4621 gttgggtgcc aaactgcgaa agctcaagcg agacaagggt aaagataaac gagactaaac
     4681 ctaaactgac caggtgaagc agcgggtggg gcagggcaat taatacatgc tgtgattgtt
     4741 tgcttattac cgctttatcc gcaaggccag gtcgccagta ttcgtttctg tgtttatgct
     4801 agtacaaaag ttttacattt tatatgcgaa ttgctcataa agtgacgtaa aggcggataa
     4861 tttgcgcaac tgcgtataac attttttacc ttacataaaa ctgatcaacg taatttgcca
     4921 ccgggaattc tttgtcaaca attaactctg agcgcgcggc aacgctattc gactggtatc
     4981 agacggatga aatccctata attgccgcgt ttggcgcttc gtcgccccct tcctaacatc
     5041 caggttaatc aggtcgctaa atatatgact gatcagtctc atcagtgcgt cattatcggt
     5101 atcgctggcg catcggcttc cggcaagagt cttattgcca gtacccttta tcgtgaatta
     5161 cgtgagcaag tcggtgatga acacatcggc gtaattcccg aagactgcta ttacaaagat
     5221 caaagccatc tgtcgatgga agaacgcgtt aagaccaact acgaccatcc cagcgcgatg
     5281 gatcacagcc tgttgcttga gcatttacaa gcgttgaaac gcggctcggc aattgacctg
     5341 ccggtttaca gctatgttga acatacgcgt atgaaagaaa cggtgacggt tgagccgaag
     5401 aaggtcatca ttctcgaagg cattttgttg ctgacggatg cgcgtttgcg tgacgaactt
     5461 aacttctcca ttttcgttga taccccgctg gatatctgcc tgatgcgccg catcaagcgt
     5521 gacgttaacg agcgtgggcg ttcaatggat tccgtgatgg cgcaatatca aaaaaccgtg
     5581 cgcccgatgt tcctgcaatt cattgagcct tctaaacaat atgcggacat tatcgtgccg
     5641 cgcggcggga aaaaccgcat cgcgatcgat atattgaaag cgaaaataag tcagttcttt
     5701 gaataagctt gataaattgt gtaccgttca gtgataagct ggtatgccct tgacgtaatt
     5761 ggcgttaagg gagtgatgcg cgaaaggaga aaatgccatg cgtctgtgtg accgagatat
     5821 tgaagcctgg cttgatgaag gccgtttgtc gatcaaccca cgtccgccag tggagcgtat
     5881 taacggcgcg acggtggatg tacgcctggg caataaattt cgtaccttcc gtggtcacac
     5941 ggcagcgttt atcgatctga gcggtcccaa agatgaagtg agcgccgcgc ttgaccgcgt
     6001 gatgagcgat gagatcgttc tcgacgaggg cgaggcgttc tatcttcacc caggagagct
     6061 g
//
LOCUS       DADLSB010000202         5960 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.202, whole genome shotgun sequence.
ACCESSION   DADLSB010000202 DADLSB010000000
VERSION     DADLSB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.202"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..149
                     /locus_tag="JN331_003752"
     CDS             <1..149
                     /locus_tag="JN331_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HAY7261753.1"
                     /translation="PDNEGHVSVRYAAANNLSVIGATKEDKQRISEILQETWESADDW
                     FVSE"
     gene            439..693
                     /gene="bssS"
                     /locus_tag="JN331_003753"
     CDS             439..693
                     /gene="bssS"
                     /locus_tag="JN331_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY7261754.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            808..1926
                     /gene="solA"
                     /locus_tag="JN331_003754"
     CDS             808..1926
                     /gene="solA"
                     /locus_tag="JN331_003754"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY7261755.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            1974..2087
                     /locus_tag="JN331_003755"
     CDS             1974..2087
                     /locus_tag="JN331_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY7261756.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2348..2914
                     /locus_tag="JN331_003756"
     CDS             2348..2914
                     /locus_tag="JN331_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY7261757.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLLRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2918..3493
                     /locus_tag="JN331_003757"
     CDS             2918..3493
                     /locus_tag="JN331_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY7261758.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3535..4587)
                     /locus_tag="JN331_003758"
     CDS             complement(3535..4587)
                     /locus_tag="JN331_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY7261759.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4812..5732
                     /locus_tag="JN331_003759"
     CDS             4812..5732
                     /locus_tag="JN331_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY7261760.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RITRWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
BASE COUNT         1528 a         1451 c         1404 g         1577 t
ORIGIN      
        1 ttcctgataa tgaaggccac gtatcggtac gttatgccgc agcgaataat ttatcggtta
       61 ttggcgcaac aaaagaagat aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa
      121 gcgccgatga ctggtttgtc agcgaataat atgcagtgat ttttgttttt gccgggtcgc
      181 cccggctttt ttattttact tacgaatttt gagtacaaaa tttctacact tcctcaaaat
      241 aattgcacaa tatgctaaaa aaatatgaat gacatgctgt tttattgttc atataaatct
      301 taaaagcaga aatttatctg ttcccccctt taaaaattcg gtgaataccc ttacttattg
      361 gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac
      421 acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta
      481 gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag
      541 accccaaata agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact
      601 gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt
      661 gatttcccgg taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc
      721 acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc
      781 ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt
      841 tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc
      901 gacgcccata tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc
      961 catgcttatg gtgaaggcga aaagtatgtc ccgttggtcc tccgcgcgca aacgctgtgg
     1021 gatgaacttt cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac
     1081 ctcggcccgg ctgactccgc atttctcgcc aacgtcgccc acagcgccga acagtggcaa
     1141 ctcaacgttg aaaagctcga tgcgcaaggg attatggccc gctggccaga aatacgcgtc
     1201 ccggacaact acatcggctt atttgagact gattccggtt ttttgcgcag cgaactggcg
     1261 attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg
     1321 gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaacggttga cggtgagtat
     1381 caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa aagacctgct cccggagctg
     1441 cctgtccagc ccgtacgtaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg
     1501 aagaataaat tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata ttatggtttt
     1561 ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca
     1621 gcggatgaac gtgttccgtt tgcggaagtg gccagtgatg ggtcagaagc cttcccgttc
     1681 ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat
     1741 aattcgcctg acgaagattt tattatcgat accctacccg gccacgataa tacactgctc
     1801 attaccggcc tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc
     1861 gattttgcgc aagaccaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc
     1921 caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc
     1981 ggctgttgca ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct
     2041 ggggattgtt ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga
     2101 cataaactga caaatattta atctcccttt catttagcgt gaaaaattga atgagattat
     2161 tcctgcttct acagcactca ccagacatct ctgatgctga acccactaaa cttatgaaga
     2221 ttctttcatt attaattcaa aattattgaa ataaaaataa atatcattca atttgttatt
     2281 tttgtgttgc aattcattct ccgacacgcc attttctttc tctcgcaaat tcgtattgga
     2341 tgcacttatg tcattcacaa atacccctga acgctatggt gttatatctg cggccttcca
     2401 ttggctaagc gcaattattg tctacggcat gtttgcttta ggtctgtgga tggtcacgct
     2461 cagttattac gatggctggt atcacaaagc acccgaactg cataaaagca tcggtatttt
     2521 gttaatgatg gggctggtta ttcgcgttct gttgcgtgtc atatctccac cgccgggtcc
     2581 gctgccgagt tattcgccca tgactcgtct tgccgcaaaa gctggacatc tcgcattgta
     2641 cctattgctg ttcgccatcg gcatcagcgg ctatctgatc tcaactgccg atggtaaacc
     2701 gatcagcgtt tttggctggt ttgacgtccc cgcgaccctt gccgacgctg gcgcacaggc
     2761 agactttgcc ggtgccctgc atttttggct cgcgtggagc gtcgtagtac tgtctgttat
     2821 gcacggattt atggccctga agcatcattt catcgataaa gacgacactc tgaagcgcat
     2881 gctgggaaag tcgtcatctg actatggagt ataaaaaatg aaaaaaagcc tgcttggttt
     2941 aaccttcgcg tccctgatgt tctctgctgg ttcagcggtt gccgccgatt acaaaattga
     3001 caaagaaggt cagcacgcct ttgttaactt ccgcatacag catcttggct atagctggtt
     3061 atatggcacc tttaaagatt tcgacggtac ttttaccttt gatgaaaaaa atccggctgc
     3121 cgataaagtg aatgtgacaa ttaacaccac cagcgtcgat actaaccacg ccgaacgcga
     3181 taaacatctt cgcagtgcgg attttctcaa taccacaaaa tatccacagg caacattcac
     3241 ctccaccagc gtgaagaaag acggtgacga actggatatt accggcgatc tgacgctgaa
     3301 tggcgtaacc aaacccgtca cgctggaagc gaaattaatt ggtcagggcg acgacccatg
     3361 gggcggtaaa cgtgcaggct ttgaggccga aggcaaaatt aagctcaaag acttcaatat
     3421 caagacagat ttaggtccag cttctcagga agtggatctg attatttcag tggaaggcgt
     3481 acagcagaag taagagtgaa atgttgccgg atggcacgca tccggcaatg atatttattc
     3541 tgttggatca ggaataccca gtgttgtatt cagacgtcca cgagacttat taaagatctt
     3601 attgccattt tcacgtcctg cccgacggcg tcgctgttct tctggcggta acgcgctttc
     3661 ttcgcagcaa atctcactac aacaaccttt gtatttttcc gcgcatactg gacactgaat
     3721 aaacagcagg tggcagccat catttttaca gttggtatgg ctgtcgcacg gcgcgccgca
     3781 ctggtggcaa tgcgcgataa tctcatccga tatacgttcg cccatccgct cgtcaaaaac
     3841 aaaatttttg ccaataaaac gcaccggcaa gccctgctcg cgcgccttac gggcgtattc
     3901 aataattccg ccctcgatat gccagacttt gttgaatccg ttatgtttca tccaggcact
     3961 ggccttttca caacgaatgc cgccggtgca gtacatgacg atttttttat ctttatgtgc
     4021 ctgcatcatc tcgactgctt ttggcagctg ctcacggaag gtatctgccg gaatttcgag
     4081 cgcgttttca aagtgcccca cttcatactc atagtggtta cgcatgtcga taaatagtgc
     4141 atcgggatcg tcaagcatgg cgttcacttc cgccgcttgc agatactcac caacattgct
     4201 ggcatcaaag tgaggatcgt caataccgtc ggcaacaatg cgatcgcgta ccttcatgcg
     4261 cagtacccag aaggatttcc cgtcatcatc caacgcgata ttcaggcgta aaccctctaa
     4321 agccgggtcg aaggcataga gctgcgcgcg aaatgtttca acattgctcg caggtacgct
     4381 gatttgcgcg ttaatgccct catgcgccag atacactcgc ccaaaaacat tcagcgcggt
     4441 aaacagctga tataaagcgt cacgggtcgc cttaggatcg gcgatgtgga aatacttgta
     4501 aaacgaaatg gtggttcgcg gttcgctctc agccaacatt ttggctttta acgcgtcgtt
     4561 ggaaatgcgg ttgtgtaaca ctggcatggt gtacggttcc tgcgagatgg gaaagtaaaa
     4621 atccgcggca tgatatagca attatcgata attaacatcc acacatttta cgctacattt
     4681 gcgcattaaa aattatttgt tatttacaag cgcggcaatt tcgcccagtc ttcagccaca
     4741 aattttggtt gcgggcgaaa aaatgcgaca atacatacaa ttgcccgtat aggttgaaaa
     4801 acaggattga tatgacgaat ctacccaagt tctccaccgc actgcttcat ccgcgttatt
     4861 ggttaacctg gttgggtatt ggcgtacttt ggttggtcgt gcaattgccc tacccgatta
     4921 tctaccgcct cggttgtgga ttaggaaaac tggcgttacg ttttatgaaa cgacgcgcaa
     4981 aaattgtgca tcgcaacctg gaactgtgct tcccggaaat gagcgaacaa gaacgccgta
     5041 aaatggtggt gaagaatttc gaatccgttg gcatgggcct gatggaaacc ggtatggcgt
     5101 ggttctggcc ggaccgccga atcacccgct ggacggaagt gatcggcatg gaacacattc
     5161 gtgacgtgca ggcgcaaaaa cgcggcatcc tgttagttgg catccatttt ctgacactgg
     5221 agctgggcgc gcggcagttt ggtatgcagg aaccggggat tggcgtttac cgcccgaacg
     5281 ataatccact gattgactgg ctacaaacct ggggccgttt gcgctcaaat aaatcgatgc
     5341 tcgaccgcaa agatttaaaa ggcatgatta aagccctgaa aaaaggcgaa gtggtctggt
     5401 acgcaccgga tcatgattac ggcccgcgct caagcgtttt cgtcccgtta tttgccgttg
     5461 agcaggctgc gaccacgacc ggaacctgga tgctggcacg gatgtccggc gcatgtctgg
     5521 tgcccttcgt tccacgccgt aagccagatg gcaaagggta tcaattgatt atgctgccgc
     5581 cagagtgttc tccgccactg gatgatgccg aaactaccgc cgcgtggatg aacaaagtgg
     5641 tcgaaaaatg catcatgatg gcaccagagc agtatatgtg gttacaccgt cgctttaaaa
     5701 cacgcccgga aggcgttcct tcacgctatt aaatctccca tgccggatgc ttcagaatgg
     5761 catccggcat taccacagca aatccccctg atttagcgat aaaagctctc tggattgcgc
     5821 cccctggaag tcgggcgcat aattagtgtg tttatctttt cttcttatgt tcaccgcgcc
     5881 tggcgcacca acagcggatt gctatgtcac cctgtgaaaa tgacacccct ataaactgga
     5941 aacgaaacct gatcgtcgcc
//
LOCUS       DADLSB010000203         5852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.203, whole genome shotgun sequence.
ACCESSION   DADLSB010000203 DADLSB010000000
VERSION     DADLSB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.203"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            163..237
                     /gene="tnaC"
                     /locus_tag="JN331_003760"
     CDS             163..237
                     /gene="tnaC"
                     /locus_tag="JN331_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY7261761.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            458..1873
                     /gene="tnaA"
                     /locus_tag="JN331_003761"
     CDS             458..1873
                     /gene="tnaA"
                     /locus_tag="JN331_003761"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY7261762.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPYIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1964..3211
                     /gene="tnaB"
                     /locus_tag="JN331_003762"
     CDS             1964..3211
                     /gene="tnaB"
                     /locus_tag="JN331_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY7261763.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3343..4518
                     /gene="mdtL"
                     /locus_tag="JN331_003763"
     CDS             3343..4518
                     /gene="mdtL"
                     /locus_tag="JN331_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY7261764.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALEIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4493..5452
                     /gene="yidZ"
                     /locus_tag="JN331_003764"
     CDS             4493..5452
                     /gene="yidZ"
                     /locus_tag="JN331_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY7261765.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLV
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPHYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5609..>5852
                     /locus_tag="JN331_003765"
     CDS             5609..>5852
                     /locus_tag="JN331_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheinyl transferase"
                     /protein_id="HAY7261766.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFS"
BASE COUNT         1383 a         1406 c         1421 g         1642 t
ORIGIN      
        1 ttttaaacat taaaattctt acgtaattta taatctttaa aaaaagcatt taatactgct
       61 ccccgaacga ttgtgattcg attcacattt aaacaatttc agaatagaca aaaattctga
      121 gtgtaataat gtagcggcgt gtctttcgag gataagtgca ttatgaatat cttacatata
      181 tgtgtgacct caaaatggtt caatatcgac aataagattg tcgatcaccg cccttaattt
      241 acccttctgt agccatcacc agagccaaac cgattagatt caatgtgatc gattagtttg
      301 ctatatctta attttgcctt ttgcaaaggc catctctcgt ttatttactt attttagtaa
      361 atgatggtgc ttgtatatat acatggcgaa ttaatggata ttgcagatgt aatattcaca
      421 gggatcactg taattaaaat aaatgaagga ttatgtaatg gaaaacttta aacatctccc
      481 tgaaccgttc cgcattcgtg ttattgagcc agtaaaacgt actactcgcg cttatcgtga
      541 agaagcaatt attaaatccg gtatgaaccc gttcctgctg gatagcgaag atgtgtttat
      601 cgatttactg accgacagcg gcaccggggc ggtaacccaa agtatgcagg cagcgatgat
      661 gcgcggcgac gaagcctaca gcggcagccg cagctactat gcgttagccg agtcagtgaa
      721 aaatatcttt ggttatcaat atactattcc gactcaccag ggccgtggcg cagagcaaat
      781 ctatattccg gtactgatta aaaaacgcga gcaggaaaaa ggcctggatc gcagcaaaat
      841 ggtggcattc tctaactatt tctttgatac cacgcagggc catagccaga ttaacggctg
      901 taccgtgcgt aacgtctata tcaaagaagc cttcgatacg ggcgtgcgtt acgactttaa
      961 aggcaacttt gacctcgaag gattagaacg cggtattgaa gaagttggcc cgaataacgt
     1021 gccgtatatc gttgcaacca tcaccagtaa ctctgcaggt ggtcagccgg tttcactggc
     1081 aaacttaaaa gcgatgtaca gcatcgcgaa gaaatacgat attccggtgg taatggactc
     1141 cgcacgcttt gctgaaaacg cctatttcat caagcagcgt gaagcagaat acaaagactg
     1201 gaccatcgag cagatcaccc gcgaaaccta caaatatgcc gatatgctgg cgatgtccgc
     1261 caagaaagat gcgatggtgc cgatgggcgg cttgctgtgc atgaaagacg acagcttctt
     1321 tgatgtgtac accgagtgca gaaccctttg cgtggtgcag gaaggcttcc cgacatatgg
     1381 cggcctggaa ggcggcgcga tggagcgtct ggcggtaggt ctgtatgacg gcatgaatct
     1441 cgactggctg gcttatcgta tcgcgcaggt acagtatctg gtcgatggtc tggaagagat
     1501 tggcgttgtc tgccagcagg cgggcggtca cgcggcattc gttgatgccg gtaaactgct
     1561 gccgtatatc ccggcagacc agttcccggc acaggcgctg gcgtgcgagc tgtataaagt
     1621 cgccggtatc cgtgcggtag aaattggctc tttcctgtta ggccgcgatc cgaaaaccgg
     1681 taaacaactg ccatgcccgg ctgaactgct gcgtttaacc attccgcgcg caacatatac
     1741 tcaaacacat atggacttca ttattgaagc ctttaaacat gtaaaagaga acgcggcgaa
     1801 tattaaagga ttaaccttta cctacgaacc aaaagtattg cgtcacttca ccgcaaaact
     1861 gaaagaagtt taattaatac tacagagtgg ctataaggat gttagccact ctcttaccct
     1921 acatcctcaa taacaaaaat agccttcctc taaaggtggc atcatgactg atcaagctga
     1981 aaaaaagcac tctgcatttt ggggtgttat ggttatagca ggtacagtaa ttggtggagg
     2041 tatgtttgct ttacctgttg atcttgccgg tgcctggttt ttctggggtg cctttatcct
     2101 tatcattgcc tggttttcaa tgcttcattc cgggttattg ttattagaag caaatttaaa
     2161 ttatcctgtc ggctccagtt ttaacaccat caccaaagat ttaataggta acacctggaa
     2221 cattatcagc ggtattaccg ttgccttcgt tctctatatc ctcacttatg cctatatctc
     2281 tgctaatggt gcgatcatta gtgaaacgat atcaatgaat ttgggctatc acgctaatcc
     2341 acgtattgtc gggatctgca cagccatttt cgttgccagc gtattgtgga taagttcgtt
     2401 agccgccagt cgcattacct cattgttcct cgggctgaag attatctcct ttgtgatcgt
     2461 gtttggttct ttcttcttcc aggtcgatta ctccatcctg cgcgatgcca ccagcaccac
     2521 tgcgggaacg tcttacttcc cgtatatctt tatggctttg ccggtgtgtc tggcgtcatt
     2581 tggtttccac ggcaatattc ccagcctgat tatttgctat ggcaaacgca aagataagtt
     2641 aatcaaaagc gtggtatttg gttcgctgct ggcgctggtg atttatctct tctggctcta
     2701 ttgcacgatg gggaatattc cgcgcgaaag ctttaaggcg ataatctcct caggcggcaa
     2761 cgttgattcg ctggtgaaat cgttcctcgg caccaaacag cacggcatta tcgagttttg
     2821 cctgctggtg ttctctaact tagctgttgc cagttcgttc tttggtgtca cgctggggtt
     2881 gttcgattat ctggcggacc tgtttaagat tgataactcc cacggcgggc gtttcaaaac
     2941 cgtgctgtta accttcctgc cacctgcgtt gttgtatctg atcttcccga acggctttat
     3001 ttacgggatc ggcggtgccg ggctgtgcgc caccatctgg gcggtcatta ttcccgcagt
     3061 gcttgcaatc aaagctcgca agaagtttcc caatcagatg ttcacggtct ggggcggcaa
     3121 tcttattccg gcgattgtca ttctctttgg tataaccgtg attttgtgct ggttcggcaa
     3181 cgtctttaac gtgttaccta aatttggcta aatccttcaa gaagccagcc attcgctggc
     3241 ttcttgcctc tcaggaaatc acttatgtcc aaatggcaac tcgccttatc ctccttcacc
     3301 acgtatgctt tgcgtcacct tactatcagg acgctttagc ccatgtcccg ctttttgatt
     3361 tgtagttttg ccctggtttt actttatccc gccgggattg atatgtacct cgttggttta
     3421 ccgcgcatcg ccgccgatct caatgccagc gaagcgcagt tgcatattgc gttctccgta
     3481 tatctggcgg ggatggcagc tgcgatgcta tttgccggta aagtggccga tcgttcaggg
     3541 agaaagccgg tcgccatacc cggcgcggcg ctatttatta ttgcctcggt gttctgttca
     3601 ctggctgaaa ccagcacgtt atttcttgca ggccgatttc tacaggggtt gggcgcaggc
     3661 tgttgttacg tggtggcgtt cgccattttg cgcgacacgc tggatgatcg acgtcgggct
     3721 aaagtgctgt cattactcaa cggtattacc tgcatcattc cggtgttagc gccagtgctc
     3781 ggacatctga ttatgcttaa attcccgtgg cagagtctgt tctgggcgat ggcaatgatg
     3841 ggcatcgcgg tactgatgtt gtctttgttt attttaaaag aaacgcgccc agcgtccccc
     3901 gctgcttcgg acaaaccacg agaaaatagc gagtcgctgc ttaatcgttt tttcctcagc
     3961 cgtgttgtta tcaccaccct cagcgtttcg gtgatcctca ctttcgtcaa tacatcgccg
     4021 gtattgctga tggaaatcat ggggtttgag cgcggagaat acgccaccat tatggcgctg
     4081 accgctggcg tcagcatgac cttttcattc tcgacgccat ttgcgctgga aatttttaag
     4141 ccacgtacgt tgatgatcac ctcgcaggtg ttattccttg cggcggggat cacccttgcc
     4201 gtttcacctt cccatgcggt ttctctgttt ggtatcacgc tgatttgcgc cggtttctcg
     4261 gtaggttttg gcgtagcgat gagtcaggcg ttaggaccat tttcattacg cgcgggcgta
     4321 gccagctcga ccttaggtat tgcgcaggtt tgcggttcgt cactgtggat ttggctggca
     4381 gcggtggttg gtatcggcgc atggaatatg ctgatcggga ttctgattgc ctgtagcata
     4441 gtgagcctgt tgctgattat gttcgtcgcg cctggacgcc ccgttgccgc tcatgaagaa
     4501 atccatcacc acgcttgatc tcaatctgct gctctgtctg caactgctga tgcaggagcg
     4561 cagcgtgacc aaagcggcga agcggatgaa cgtgacccct tcagcggtga gtaagtcgct
     4621 ggtaaagtta agagcgtggt ttgacgaccc gctctttgtg aactcaccgc tggggctgtc
     4681 gcccacaccg ctaatggtca gcatggagca aaatctggcg gaatggatgc aaatgagcaa
     4741 tctgctgctg gataaaccgc accaccagac accgcgcggc ctgaagtttg agttggcggc
     4801 ggaatcgccg ctgatgatga tcatgcttaa tgcgctgtcg aaacggatct accaacgtta
     4861 cccgcaggcg accatcaaac tacgtaactg ggattacgat tccttagatg ccattactcg
     4921 tggtgaagtg gatattggtt tttccggtcg cgaaagccat cctcgctcgc gggagctgtt
     4981 aagctcgcta ccgttagcca ttgattatga agtgctgttt agtgatgtac cctgcgtctg
     5041 gttacgcaaa gatcatccgg cactgcatga aacgtggaat ctggacacct ttttacgtta
     5101 tccgcatatc agcatttgct gggaacagag cgacacctgg gcgctggaca atgtgttaca
     5161 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaattcgagc agtcactgtt
     5221 tatggcagcg caacccgaca atctgctact ggcgaccgcg ccgcactact gtcagtacta
     5281 caatcaactc catcaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca
     5341 aaaaaagctg gaagttcctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa
     5401 gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg
     5461 tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa
     5521 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
     5581 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
     5641 ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat
     5701 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
     5761 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt
     5821 tttcagtgat aaaaatttgc cttcgttttc ta
//
LOCUS       DADLSB010000204         5848 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.204, whole genome shotgun sequence.
ACCESSION   DADLSB010000204 DADLSB010000000
VERSION     DADLSB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.204"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..956)
                     /locus_tag="JN331_003766"
     CDS             complement(<1..956)
                     /locus_tag="JN331_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7261767.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGS"
     gene            1203..1829
                     /locus_tag="JN331_003767"
     CDS             1203..1829
                     /locus_tag="JN331_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7261768.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1826..2941)
                     /locus_tag="JN331_003768"
     CDS             complement(1826..2941)
                     /locus_tag="JN331_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7261769.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(3052..3435)
                     /gene="bssR"
                     /locus_tag="JN331_003769"
     CDS             complement(3052..3435)
                     /gene="bssR"
                     /locus_tag="JN331_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY7261770.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSFTLSIQSLQKHAMLEKA"
     gene            3648..4973
                     /gene="rimO"
                     /locus_tag="JN331_003770"
     CDS             3648..4973
                     /gene="rimO"
                     /locus_tag="JN331_003770"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY7261771.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5020..>5848)
                     /locus_tag="JN331_003771"
     CDS             complement(5020..>5848)
                     /locus_tag="JN331_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7261772.1"
                     /translation="SLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYL
                     PTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNH
                     FERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFD
                     VDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEEL
                     LSILKEIVHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
BASE COUNT         1482 a         1519 c         1390 g         1457 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgctcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atcggccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcgggatc tagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaaatac gttatatcgc cgtatgataa gttcggtgat
     1021 taataacgca cacacaaact taaagaaacc gtaagttggt aaagttttta aagtttatgc
     1081 aatatcctgc cgcttcgctg cttctggtgt catttttttg ccagctgttg cttaaaagtt
     1141 aactttactt caccatcgga gtcctgttca gtctgtggct gcctgtcgga aaaggggtga
     1201 gtatgattac gctgtggggt cggaataatt caactaacgt aaaaaaagta ttgctgacgc
     1261 tcgaagaact ggaactacct tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc
     1321 acgatgctga ttttctggcg atgaacccta acgggctggt gccgttgttg cgtgacgacg
     1381 aaagtgatct cattctttgg gaatcaaacg ccattgtccg ctatctggcg gcacagtacg
     1441 ggcaaaaacg cctgtggatc gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg
     1501 actgggcaaa ccagacgctc agtaatgctc atcgcgggat cctgatggga ttagtcagaa
     1561 caccaccgga agagcgcgat caggccgcca ttgatgccag ttgcaaagag tgcgacgccc
     1621 tgtttgccct gctcgatgcg gaactggcaa aagtaaaatg gttctctggc gacgagtttg
     1681 gtgtgggcga tatcgctatc gcaccgttca tctacaattt gtttaacgtt ggcctgacct
     1741 ggacaccgcg tccaaatctg caacgctggt atcagcaact cactgaacgc cccgcagtgc
     1801 gcaaagtggt gatgattccc gttagctaat tgcgtgggct aactttaagt aattccccac
     1861 tggactcgtc ggtgagaacg tataaataac cgtcgggtcc agtgcgaaca tcacgaattc
     1921 gctgccctct gtccgttaaa atacggccat cttctgtcac tttgtcgccg ttgacgctca
     1981 tcacaatgac atctttatct ttcagcgcgc caataaataa tttttgctgc cactggggga
     2041 atttatcgct gttatagaag gccatgccgc tcacagcggg cgaatctttc cagtaaaaaa
     2101 caggttgctc ggtcccggcg acgatctccc ctttcgcttc cggtatctta aagcctgaat
     2161 agttgattcc ccaggttgcc agcggccagc cgtagttttt gcctttttgc gggatattaa
     2221 tttcatcacc accgcgcggg ccatgttcat tcagccacag tgcattactc cacggattca
     2281 tcgccattcc ttgcggatta cgaatgccat aagaccagat ctcggcgcgc gcaccggatt
     2341 cctttataaa aggattatca tccgggattt cgccctggtc ggtcagacgc accagtttgc
     2401 cctgtaattt atccagatcc tgcgccgtcg ggcgctgatt gttttcgccc agagcaataa
     2461 aaagataacc tttaccgtcg aataccagcc gcccgccaaa atggttgccg gtagacagtt
     2521 ttggcatctg gcggaagacg gtgcggaagt cggtcacttt tgagagatca tcacttaagc
     2581 ggccataacc cacagcagtt ccggctttgc catcatcgcc aacttcggaa taacttaacc
     2641 agatgcggcg agactgagca aaatcaggcg ctaaaaccac gtccagcagg ccgccctgcc
     2701 cgtgcgccca aacgtccgga actccggaaa gcggcgcaga taatcctttt cctgcttgcc
     2761 agtgacgcaa ctcgccgccg cgcagagtga ttaacatacc gtgattatcg ggtaaaaagg
     2821 ccagtgccca gggatggtcg agtttgtctt gcagtacttc gacatttacc gttgcaggag
     2881 ccgcccagag agcggaagaa agacaaataa ggggcacaag gaaaaaggat tgtcgatgca
     2941 taacgcctcc ggattgactc tggcttaaag cgtagtcagt ggaggagata acaaattcat
     3001 ttttacaaaa acttaaacat gaagggggag acgctttctc ccccttagtt ttcaggcctt
     3061 ctcaagcatg gcgtgcttct gcaggctctg gatactcagc gtaaagctca tcagacaatt
     3121 ttcaagctta tcggcgttga cggtaataac agtcgggcaa tcatggtgcc cactcatcaa
     3181 acatactgcg gctgtcgcta atgcttcttc agcatgatga agagcactcc actcttcctg
     3241 atccagatga agattcaacc gcagcgattt atcgtgcagt tcgcgattca gtttaaaaaa
     3301 gttatctcgt agatgattgc tttcgctgac ggacatgtat ccttttgcct tcctcagttg
     3361 cacgtatgca gcgaggtcaa cgcgtgcgtc gatcaaccgg ttcagcagat cgattcgctg
     3421 tctgtcaacg aacatacttc gatcctcctc tttccagcct tgttgcaatt aaatagtatg
     3481 gctatttttt gagcaaaatg ctgtccagga tcaattcttc acgccgaatt aacaaaattt
     3541 acatttcctt cgcctccgcc agcgaatgag attgcgatac aaattttatc cagaactctg
     3601 tagaatccct gccctgacct ttcctgtaac tgaatatttt ttaagctatg agcaaagtaa
     3661 ctccccagcc gaaaatcggc tttgtttccc ttggctgtcc gaaaaacctt gtcgattcag
     3721 agcgtattct caccgaactc cgcactgaag gttatgacgt ggtaccgagc tatgacgatg
     3781 cggacatggt gatcgtcaac acctgcggct ttattgacag cgcggtacaa gaatcactgg
     3841 aagccattgg tgaagcgttg aatgaaaacg gcaaggtaat tgtgaccggt tgtctggggg
     3901 caaaagaaga tcagatccgc gaagtccacc cgaaagtgct ggaaatcacc gggcctcata
     3961 gctatgagca ggttctggag cacgttcatc actacgtgcc aaaaccgaaa cacaacccat
     4021 tcctgagcct ggtgccagaa caaggtgtga aactgacgcc gcgtcattat gcctatctga
     4081 aaatttcgga aggctgtaat caccgctgca ccttctgcat tattccgtct atgcgcggcg
     4141 acctggtgag ccgtccgatt ggcgaagtgt taagtgaagc gaaacgtctg gtagatgcgg
     4201 gcgttaaaga gattctggtg atctcgcagg atacttccgc ctatggcgtt gatgttaaac
     4261 atcgtactgg cttccacaac ggcgagccgg taaaaaccag catggtcagc ctgtgcgaac
     4321 agttatcgaa actggggatc tggacacgtc tgcactacgt ttacccttat ccgcatgtgg
     4381 acgacgtcat cccactgatg gcagaaggca aaatcctgcc gtatctggac attccgttgc
     4441 agcacgccag cccgcgcatt ctcaaactga tgaagcgtcc gggttctgta gatcgccaac
     4501 tggcgcgcat caaacagtgg cgcgaaatct gcccggaact gaccctacgc tcaaccttta
     4561 ttgtcggctt ccctggcgag acggaagaag atttccagat gctactcgac ttcctgaaag
     4621 aagcacgtct ggatcgcgtt ggctgcttta aatacagccc ggttgaaggt gcagacgcca
     4681 atgccctgcc tgaccaggtt ccggaagaag tgaaagaaga acgctggaac cgtttcatgc
     4741 agctgcagca gcagatttcc gccgagcgcc tgcaagagaa agtgggccgt gaaattctgg
     4801 tgattatcga cgaagtggac gaagaaggcg cgattggtcg cagcatggca gatgcaccgg
     4861 aaatcgacgg cgcggtttat ctcaatggtg aaaccaacgt taagccgggt gatatcctgc
     4921 gtgtgaaagt cgagcacgcc gatgagtacg atttgtgggg tagccgggtt taattaattc
     4981 aggccgggca aaatcatttg tccggcctgc gaataatgat tactgccagc taatctgtgc
     5041 cctgccgttt ttcttactct tatacagccg ctcatccgcc agcattttca agcgttcgac
     5101 ggtagtacat tcatttgaat gcgctacacc gatactggtg cttaaattaa tgcttcccac
     5161 ctggaaatga acgatttctt ttagtatcga tagcaactct tccagcgtgc cgccagtata
     5221 aatcaccgca aattcgtcac ctccgaagcg gtagacttta cctttatcta ccgggatctg
     5281 tgatttaagg attttcacca ctttcatcaa cacttcatcg cccagcaaat gaccgaaggt
     5341 gtcgttaatg tttttgaatt tatcgacgtc aaacagacac aaataaccgc ctttttccgg
     5401 cagcgctttt aaatcttcgt caaaggattt tctgcccatt gctttggtca ggtcgtccag
     5461 gaacgccgag ttatacatac tgtagagttt tttgacatcg gcagccgaaa agaaaagccc
     5521 gccatccagt acgcgttcaa aatggttatt aatgatggct tctttctcca caataccacg
     5581 attaatcact ctgcgtacca gatagaggca actggcaccc gatatcactg ccaccaatgc
     5641 ggcaaagaaa ataatcaatg tacaaacccg caaataaaca tcttttttat acgcaaatac
     5701 gtacaatccg gtcggcaaat aggcgaagtg ctccatcgct tccgaaacgg tgaagatggc
     5761 gtttttagca ttatattcat caccggtcac cggtttaccg ttcttaaata gagagaacat
     5821 caggttctcg ctgtccatcg ccagcgat
//
LOCUS       DADLSB010000205         5780 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.205, whole genome shotgun sequence.
ACCESSION   DADLSB010000205 DADLSB010000000
VERSION     DADLSB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.205"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..464)
                     /locus_tag="JN331_003772"
     CDS             complement(<1..464)
                     /locus_tag="JN331_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY7261773.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTKQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVK"
     gene            complement(511..1230)
                     /gene="yehT"
                     /locus_tag="JN331_003773"
     CDS             complement(511..1230)
                     /gene="yehT"
                     /locus_tag="JN331_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY7261774.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1227..2912)
                     /gene="btsS"
                     /locus_tag="JN331_003774"
     CDS             complement(1227..2912)
                     /gene="btsS"
                     /locus_tag="JN331_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY7261775.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVHLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3134..3199
                     /locus_tag="JN331_003775"
                     /pseudo
     CDS             3134..3199
                     /locus_tag="JN331_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            3427..3675
                     /locus_tag="JN331_003776"
     CDS             3427..3675
                     /locus_tag="JN331_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7261776.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(3791..4060)
                     /locus_tag="JN331_003777"
     CDS             complement(3791..4060)
                     /locus_tag="JN331_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7261777.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            4117..4785
                     /locus_tag="JN331_003778"
     CDS             4117..4785
                     /locus_tag="JN331_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7261778.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            5101..>5780
                     /locus_tag="JN331_003779"
     CDS             5101..>5780
                     /locus_tag="JN331_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7261779.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT         1624 a         1397 c         1232 g         1527 t
ORIGIN      
        1 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
       61 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      121 acgcggaact gctgtttggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      181 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      241 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      301 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      361 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      421 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta gcatgaggcc ttcaggtgtt
      481 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
      541 cagataacgg cggctgaccg gcacggttaa accattacgc aaaatcaact cggcctggcc
      601 gttatcttcc aaacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
      661 gcgcagtagt ggtgtacgac tttccagggt acgtaatgtc aattcggtaa agccctcttt
      721 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
      781 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
      841 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
      901 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
      961 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1021 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1081 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1141 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1201 ttcatcatca acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1261 attcgggtgt aactatcagg ctcacaggcg acgcttattc cataatcatc gccaaaccgt
     1321 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1381 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1441 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     1501 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     1561 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     1621 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     1681 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     1741 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     1801 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     1861 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa acggtttttg
     1921 gcttcataca atttgatggt gcccatcaca cgctgatttt cgccacgcaa cggaattacc
     1981 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2041 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2101 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2161 gtaatcgcga ctgcaccaat atccagctcc tgatacagca cctgagccac tttcatgctg
     2221 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2281 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2341 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2401 agatgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     2461 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     2521 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     2581 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     2641 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     2701 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     2761 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     2821 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     2881 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     2941 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3001 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag atgcacaaaa
     3061 aacgggcaaa actacctggt tcgcaaaact gcgtctaaag ttaaactggg acctcgcgag
     3121 caagggtgag atgatggcgc tttacacaat tggtgaagtg gcgttgcttt gtgatattaa
     3181 tcctgtcacg ttacgcgcgt gacgcaacat ttttaaaaat cgacgaagat cgtccaggag
     3241 cgccgttttt caagggttgg atagatgatt cgtttaccat gtcgacgaac ttcgacgaat
     3301 ttcgccgata aaaagtgtcc catacatgcc ccattgatgc cccataaaca atatcaggaa
     3361 ttgacactgg ttatccatac agataaaaat aacacctgta cataactaca gttacaggag
     3421 ggtgttatgc gtgttgagat ctgtatcgct aaagaaaaaa tcactaaaat gccaaacggt
     3481 gctgtggatg cgttaaagga agaattaacc cgacgcatca gtaaacgtta tgacgatgta
     3541 gaggtgatcg taaaagccac cagcaacgat ggcctttctg ttacacgcac cgcagataag
     3601 gattctgcaa aaacatttgt tcaggagact ctgaaagata cctgggagtc tgctgacgag
     3661 tggtttgttc actaataaac acgtaaaatc ggtaacggct ggaaatcatt caatactcgc
     3721 actatcgaaa gtttaccagc caaccgcagc acgtcctgca tacgtcgtgt ctgcggtttt
     3781 tctttttcgc ttacattgtg tctggttctt ccggccactc aatatcaggt gcagttgatg
     3841 tatcaacacg gttcagcaac acccgatact tcttccaggc ttccagcaac gagttttctt
     3901 cctccgttgc gatctccaga tctacagcat cctgaagtgg cgcaatatgc tcactgaatc
     3961 cctggatgta gaactgtgtg gtgacggtct tccagccatt cggctcctgc tgtatcgaag
     4021 cataccaggc tatttcaata tcgctatgct gcggcagcat ttaacccctt gtaattcatc
     4081 gccataattg atttaattca caaataaaac tataatatgg tgaaattaat gaaaaaaaac
     4141 acagatgatg gggctaaaat ttacacacca cttaccctaa agctttatga ctggtgggtt
     4201 ttgggagtat caaatcggct tgcatgggga tgtcctacaa aggaacacct tcttccacac
     4261 tttctggaac atttaggtaa caaccatctg gatattggtg ttggaactgg gttttacctt
     4321 actcacgtac ctgagagtag tctgatatct ttaatggatt tgaacgaagc tagcctgaac
     4381 gcggcatcta caagggctgg ggaatcaaaa attaaacata aaattagcca tgatgttttt
     4441 gaaccttatc ctgcggcgtt acatggtcaa tttgattcca tttccatgtt ttaccttctt
     4501 cactgcctgc ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc ggcgcaggcc
     4561 ttaactgatg atggaactct atacggagcc acaattcttg gcgatggagt tgtgcacaat
     4621 agcttcggtc aaaaactgat gcgcatttac aatcagaaag gcatcttttc aaacacaaaa
     4681 gattccgaag aaggcttaac acatatactc tcagagcatt tcgagaatgt taaaaccaag
     4741 gttcaaggta ctgtagtaat gttttccgct tcagggaaaa aatagcatcc aaccgcagca
     4801 cgttcttgct taagacgtgc tacggtttcc tttacgattt ggggttgcga ctttacctct
     4861 attgataatg cattccggca gaacgttcaa atatgaacgt tcgatattta actaaccgaa
     4921 aaacaaaata tagcacaggc gagacattta ccatctaaga aaatagtatc gtttttttac
     4981 agttaaatca acatcacttc cttaaaatga aaacaataaa tttcaatcct gaaacatttt
     5041 atttcagttt attatttccc agtatcacat tttatcagaa tatccttctg cgaggttatt
     5101 atgcttcctg taaataatcc ccccctatcc actggaaacg tctcttttta cagaactaca
     5161 tcaatcgaca atgttcacaa taattatctc tccgaatggg ttgaatggac taaaaacagc
     5221 atttccggag aaaacaggga aactgctttt acccggctcc aattatgtct ggagaacagt
     5281 gaaacatcgt tggacttatc ttgtttaggt ctcagatctc taccacgatt gcctgacaat
     5341 cttgatgaaa ttaatgtaag caataaccaa ctatcaatgc tccccgagct accaagggca
     5401 ttgaaagagc tgaatgcaag cagtaatcaa ttatctgcac ttcctgaatt accagtgtcg
     5461 ctggaatata taaatgtgag tgataaccat ttgttcgcac ttcctgaatt acctgcgtca
     5521 ctagaatata ttaatgtaag tgacaatcac ctgtctgtac ttccgaggtt accaatgtca
     5581 ttggaattac ttgatgcagc cagaaatgct ttggaagtaa taccagattt tccagaaaga
     5641 gatgatcata ttataagaat attctggctt aatcagaacc ggatcacggc aattccggaa
     5701 agcatacttg gcctcagttc tgatagcgtt gtcaatctta gagaaaatca actatctccc
     5761 agaataatgc aaactttgtt
//
LOCUS       DADLSB010000206         5772 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.206, whole genome shotgun sequence.
ACCESSION   DADLSB010000206 DADLSB010000000
VERSION     DADLSB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.206"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(1..391)
                     /locus_tag="JN331_003780"
                     /pseudo
     CDS             complement(1..391)
                     /locus_tag="JN331_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(577..3885)
                     /gene="icsA"
                     /locus_tag="JN331_003781"
                     /gene_synonym="virG"
     CDS             complement(577..3885)
                     /gene="icsA"
                     /locus_tag="JN331_003781"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY7261780.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPVAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINTDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSMINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKDRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4414..5616
                     /gene="virA"
                     /locus_tag="JN331_003782"
     CDS             4414..5616
                     /gene="virA"
                     /locus_tag="JN331_003782"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY7261781.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1784 a         1310 c          955 g         1723 t
ORIGIN      
        1 aggctgtacg cttccccatg tgatttgcct cgccacttca ccgggctcag gaccccgtag
       61 gatcttcacc gaaaaaaatg catcaccagt tgctttatta tttgttttag gtagacctgt
      121 tactaattta tacctactct gaccaagtct ggataagaaa ggcacacctg cacatttcta
      181 cttggaaatg ttttctttta ccattttgct caaaaatttc aacatccaat attcctcgta
      241 cagaactact caagtcctga atagaaaaag ttccagctgg tacagtagag tcatatatta
      301 ctcgaccttg ttgagataca acaacacgtg cattagtttc tgcaatacca gtaatctgag
      361 gcgcataacc acgaatattt ggcggtagca ttcgttcatc gctttctaat gcaattcctg
      421 tgtaacgcca agcaacagcg cattctggcg catgatctgg taaacacgtt tggcattgat
      481 cgcaggcata ccatcatgtg cacaaaaacc caccgagctt ttacacggtg ggtcccagag
      541 aaatgcagga catcaacacg ccctgcattt ttattatcag aaggtatatt tcacacccaa
      601 aataccttgg gtgtctctgt aactgttatt acccaattgc tgagatacgt ttccccagat
      661 gcttaggtta tctgcaagac gcccccccac accagttttg agttcaatca cattacggtt
      721 gcctatctgg tgatttgaca tgccattcat aattacacca tattgctttg agctccattt
      781 ccaattcact tccaaaaatg gttcgaactt acgaagagtc tccttatcga tacaactctg
      841 actaattaag gatgtacgca atcccaattt tgcttgaata ttattgaccc cagaggatgt
      901 gaccatagtg ccattgtgtt caatatagtc gtttgctttt accccatgcc tagttatctg
      961 gacttgtgga ttgagataca atgcgttgtc aatattatta tgagcagtcc agcgtatggt
     1021 tgggtagata tagccagctt ccaaagctcc tgttattcct ttgctggaat atttctcacc
     1081 agttaaccca tctcctttga ctgtattatt aaaccaaccg tactgcaacc aagcatccat
     1141 atatagacct gttcgttctt tttcatcctg aaaccaagaa ctgtacaaac cggcagtata
     1201 accgctcaca gttcctcgtg aactataact agtatactta ttatgagttt tgctatcctg
     1261 atatccataa gctcccataa tacccacatg caagccatca tgatcagtga agtttgtctt
     1321 caaaatatct ccaccaatct gataaaccat cctgttagct gtggttttta attgtctatc
     1381 agctaaatta tgctcattat gtccaccaac agtacgaatc cacatggtgg tttcgctgag
     1441 ctgacctgta gtaggatcaa caatagtgct accgtgtcgg tcatagagag aaggactaaa
     1501 catcgtgttc attgctatca ggttagcaat ataactgcca gcttcagggc gccatgtgtg
     1561 aatactacct tcacccaaat taagtgaaga tacaagctgt gaactagctc ggcgactact
     1621 catttgagta gactcttgat tgctcatctg ttttgattct tgattatcca tctgactagt
     1681 tagataccac ttattggtat tcagtccaga tacagtgccc tgtttcaggc gatagtcata
     1741 actaccagca acaatacggt ctttctgaat aaaagcattc ttatcagaag aatctgtcga
     1801 aataatttga acaccttcaa gagttttatc tccagagcct ccctcatttg tcactgcaac
     1861 ataggtaata ccagacgcat ggcctgcaat ttccagccgg tcagttttac tgttgtcatt
     1921 gccgagaacc gcgcccaaag ataatattcc gccctttcca tgccaattac ctttctgaac
     1981 ataggtctga ccgacatttg acgaactatt attgagaata acatgaccgt ttttctccag
     2041 agtcatgtta cctgtgacaa tgacggggtc aggcaaaaaa ggggcattaa tattattgat
     2101 tagcacagtc ccactattta ataaagtgtt aacagtttgg ttcatgcctg aaaaattcaa
     2161 ggtagcacct ttattaacaa taacaccagc ggtacgtgtc atagcttcaa ctgtccccat
     2221 ttttagaatg cctgcattga tgttggtgaa accagagtag gtattttttt ccgccaaaat
     2281 gagagttccc tcccctgatt ttgttaggga ttttccgtcc cagctattta tattatgatg
     2341 gctgagatta tctgccagaa tagtactaac agtaagttca gcgttttctt tcagattgaa
     2401 ctctccatga ctgtcgccat ctgtatcatt ccagctcaaa ccgtagatca atttattctg
     2461 gtcactgata tagctttttg tatagtcaat agcaccagta gtgagtaacg gattgatgat
     2521 agatatatta gaaacactga aacctgttat ccctttatct gcacttagca gtttatagtc
     2581 cttctgcgcc cagttattta taatagcttc cacttttaca ttgttgccaa atgacatatt
     2641 tccagaagct tccagactac tgattccagc taaatttaaa gaactgttct caccaaaaga
     2701 aacgtcacct gaaacagtga atttgttacc tgatagtgaa acagttgtac aatcaccagc
     2761 acataaatca ccttcaatag ttggtgtggt cccagtgtaa tcattgttga taattttcag
     2821 aatggatgaa ttgttaacct gtacatcacc agtaatgaca gaaccttcat ggagtattaa
     2881 tgaattgttt ccaccagtga tgtggatagc attacctgct acattttgtg ttccatcatc
     2941 ttctttacct gaaattatat atccgctatt agcaatggtc atattgctac catttatagc
     3001 atccccccaa tgtccaccat taccgcctaa aatagctcca ttgttgatca tagacaggtt
     3061 atctcctgtg atagcattac caccgtagcc atcataacca taaccgtaag cagcacctcc
     3121 gttgcctcca gcgaacgtgc ccttattgat tacagagagg ctgcttcctg tgatggcatc
     3181 acctccatta ccgccatcac cctcaccata attgttacct ccattaccac cggaaatagt
     3241 ccctttgttg attatgggta gattgactcc tgtgacggca tcacctccat tccccccatc
     3301 actatcacta taactatcgc caccatgtcc acctgaaata atttctccat ttattataaa
     3361 cagattgtct cctgtaacag cctcaccacc atcaccgtta tggtcagcac cgctaccgcc
     3421 gctaccacca agaatcatgc cttgattgat tatagacaag tcagatccgg taatagagtc
     3481 accaccatta ccgccacaac tattaccatc attattatca ccagcaccac catgacctgc
     3541 gctaatcata atatcagtgt taatccgtag cttagttcta gatgcatgag aggggttgga
     3601 agaagttata taccacgcat ccattccatc ttcaccagct cccaagggag aaagtccatc
     3661 aacaggtgtt aataattttt cataattgtc ctctgaaaaa tgaagttctt gagtacccga
     3721 aagaggaata gcaaaagcta ctggcccccc gagcaacagg gatgcaccaa caacaaaagg
     3781 agaaaaagac aatttgcatg ttttttcctt taccgtaggt aattctccgg cccccccctg
     3841 tgaacattgg gtcatattac aaaaaaattt gtgaatttga ttcatgcact atattatcag
     3901 taagtggttg ataaacccct aaaaagattc cgttttatcc ggaataaagg gacgatatat
     3961 gcaaaaacat attaaacaaa gcctcaacca aaacatgaga gctgtgttat atatcaatgc
     4021 aacgggagaa atttacctgt gtcttgcttt gaaaaaaaga gggggaaagc tatatgttcg
     4081 ataataaatt aataatttta ctatgatagt ctgcttcaga ccgcaacttt tcagttcttt
     4141 ctgtacgctt gcccaaagtt taaccacttt gtttttctat acacccaatg gttctatctg
     4201 ccccttgatg ttaatcccca aagccaataa catgaatgta ttcatatcac aactttccta
     4261 ccagactctc caatgcaata actgcataat tgtttttagt ggaaatgtat cgttttctta
     4321 aagagaagaa taacattcca ctttattatg ttcccaattt aattatatta atatatattg
     4381 gcattaatag gaaaatacat caggagaaat caaatgcaga catcaaacat aactaaccat
     4441 gaaagaaatg actcatcatg gatgtcaact gtcaaatcaa ctacagaagt aagttggaat
     4501 aaattgagtt tttgtgacgt tttactgaaa ataataactt tcggcatata ttctccacac
     4561 gaaactctag ccgaaaaata tagcgaaaaa aaactaatgg atagtttctc accatcacta
     4621 tcacaagata aaatggacgg agagtttgcc cacgcaaaca tagatggcat ttcaatacgg
     4681 ctatgcctta acaaaggcat atgttcggtt ttttatcttg atggagataa aatacaatcc
     4741 actcaacttt catcaaaaga atataataac ttgttaagtt cactcccccc caaacaattc
     4801 aacttaggaa aagtacatac tattacagct ccggtatcag ggaattttaa aactcacaag
     4861 cctgcaccag aagttattga aacagccatt aattgctgta cctcgataat accaaacgat
     4921 gattacttcc ccgttaaaga cactgatttt aatagtgttt ggcatgatat ttacagagat
     4981 attcgagcat ctgacagcaa tagtacaaaa atatatttca acaatattga aataccacta
     5041 aaattaattg cagatctaat taatgagtta ggaattaatg aattcattga tagcaaaaaa
     5101 gagcttcaaa tgctaagtta caaccaagta aataaaataa tcaattcaaa cttcccccaa
     5161 caagacctat gttttcaaac agaaaaactt ttgtttactt cactctttca agaccctgca
     5221 tttatatctg cactaacatc tgcattctgg caatctcttc acatcacgtc ttcctctgtg
     5281 gaacacatat atgcccaaat tatgtccgaa aacatagaaa acaggcttaa tttcatgcct
     5341 gaacaacgag ttattaacaa ttgtggacat attataaaaa tcaacgcagt tgtccctaaa
     5401 aacgacactg caatctctgc ctctgggggg agggcttacg aagttagctc atcaatatta
     5461 ccatcgcaca taacatgtaa tggtgttggt attaataaaa tagaaacaag ctatctcgtt
     5521 catgctggaa cacttccaag cagtgaaggg cttcgcaatg ctatccctcc agaaagtcgt
     5581 caagtatcat ttgccatcat atctcctgat gtttaagcca atattatatt ggctgccaga
     5641 ctgtaaaaaa acgttcttca gaacagctct ataaactatt aaaccaaagc taaaaggatg
     5701 gggaccaata gaatctcagc ccatcctttt tatatcatta tgaaccgccc cggaaatcct
     5761 ggagactaaa ct
//
LOCUS       DADLSB010000207         5712 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.207, whole genome shotgun sequence.
ACCESSION   DADLSB010000207 DADLSB010000000
VERSION     DADLSB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.207"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1537)
                     /locus_tag="JN331_003783"
     CDS             complement(<1..1537)
                     /locus_tag="JN331_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAY7261782.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPMQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHFSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAM"
     gene            complement(1744..2028)
                     /gene="ihfB"
                     /locus_tag="JN331_003784"
     CDS             complement(1744..2028)
                     /gene="ihfB"
                     /locus_tag="JN331_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY7261783.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2188..3861)
                     /gene="rpsA"
                     /locus_tag="JN331_003785"
     CDS             complement(2188..3861)
                     /gene="rpsA"
                     /locus_tag="JN331_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY7261784.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3972..4655)
                     /gene="cmk"
                     /locus_tag="JN331_003786"
     CDS             complement(3972..4655)
                     /gene="cmk"
                     /locus_tag="JN331_003786"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY7261785.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4828..5592)
                     /locus_tag="JN331_003787"
     CDS             complement(4828..5592)
                     /locus_tag="JN331_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7261786.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1447 a         1508 c         1346 g         1411 t
ORIGIN      
        1 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
       61 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
      121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
      181 aacattcagc aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
      241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
      301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
      361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
      421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
      481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
      541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
      601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
      661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
      721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
      781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
      841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
      901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
      961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
     1021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
     1081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
     1141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagatattcg ccatacagca
     1201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
     1261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
     1321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
     1381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
     1441 tcagaaacgc aagggttaat gtcccaggca attggggcaa aatcagcaac ggaaaaattc
     1501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
     1561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
     1621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
     1681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
     1741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
     1801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
     1861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
     1921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
     1981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
     2041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
     2101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
     2161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
     2221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
     2281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
     2341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
     2401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
     2461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
     2521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
     2581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
     2641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
     2701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
     2761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
     2821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
     2881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
     2941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
     3001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
     3061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
     3121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
     3181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
     3241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
     3301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
     3361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
     3421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
     3481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
     3541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
     3601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
     3661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
     3721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
     3781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
     3841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
     3901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
     3961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
     4021 acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
     4081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
     4141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
     4201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
     4261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
     4321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
     4381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
     4441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
     4501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
     4561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
     4621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
     4681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
     4741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
     4801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
     4861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
     4921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
     4981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
     5041 aattgcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
     5101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
     5161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
     5221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
     5281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
     5341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
     5401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
     5461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
     5521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
     5581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
     5641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
     5701 ccgacaatgt gc
//
LOCUS       DADLSB010000208         5710 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.208, whole genome shotgun sequence.
ACCESSION   DADLSB010000208 DADLSB010000000
VERSION     DADLSB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.208"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(496..801)
                     /locus_tag="JN331_003788"
     CDS             complement(496..801)
                     /locus_tag="JN331_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261787.1"
                     /translation="MGSKIAGVVFLYFAVWGMASVIELQYWRNADTYPVWLTKHLRIS
                     PENNDKSLIRIEMETFDKVCGDKRGLLAVTQKQNGMFMRCDDSPGFFSWFSGVYKVN"
     gene            complement(855..1106)
                     /locus_tag="JN331_003789"
     CDS             complement(855..1106)
                     /locus_tag="JN331_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAY7261788.1"
                     /translation="MTNIILCDVTASVSELKNDPVATASARGGYPVEIIDRNRPVFYC
                     VPAALYEQMLDELDEKDLVQTITERQNQPLREVDLNQYL"
     gene            complement(1185..2456)
                     /locus_tag="JN331_003790"
     CDS             complement(1185..2456)
                     /locus_tag="JN331_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY7261789.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRGKPVVVLSNNDGCVIARSAEA
                     KRLGIKMGTPWFQLKEAQFPEKLYVFSSNYELYASLSNRVVALLEELSPRVEQYSIDE
                     CFLDARGISHCMDLEDFGRQLRGHVLSGTGLTIGVGFGATKTLAKSAQWASKEWPQFS
                     GVLALSPENPRRTAKLLSLQPVEEIWGVGNRIAKKLHVMGITTALQLSLTNPTFIRKN
                     FNVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGQRITTYGEMRQAVCQYAERAA
                     EKLRGERQYCRHISTFIKTSPFAVNEPYYGNVATEKLNTPTRDTRDIIAAAVRSLDRI
                     WLDGHRYAKAGIMLNDFSPNGVAQLNLFDDVQPRPHSDALMKVLDGINHSGLGKVWFA
                     GRGIAPDWQMKREMLSPAYTTRWKELPVARF"
     gene            complement(2456..2881)
                     /gene="umuD"
                     /locus_tag="JN331_003791"
     CDS             complement(2456..2881)
                     /gene="umuD"
                     /locus_tag="JN331_003791"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7261790.1"
                     /translation="MKFIPAVSEASIVKIPLFTERCPAGFPSPAANYTESELDLNEYC
                     IHRRHSTYFVRAIGNSMTDIGLYSGDLMVVDKAEQPRHGDIVIAEIEGEFTVKRLLLT
                     PRPALQAMNPDFPSLYPDPETLQIFGVVTAFIHKTRRAD"
     gene            2982..4202
                     /locus_tag="JN331_003792"
                     /pseudo
     CDS             2982..4202
                     /locus_tag="JN331_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
     gene            4657..5622
                     /locus_tag="JN331_003793"
     CDS             4657..5622
                     /locus_tag="JN331_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HAY7261791.1"
                     /translation="MTMNLHTQTTAPKASQATSVSPLEQSGSSKTKFSRGKSKARAGA
                     VKTDLYQTVTDSIIAALESGVKPWVCPWVRNGAAVGLPANFSTGTAYSGINIMLLWCS
                     AAKQGFQDERWLTYKQAQELGGQVRKGEHGTTAIFYKTLEKEDEDGEIEKIPMLKAFT
                     VFNVEQIDGLSIESVPQPVAGFDPLPQAEALMIRSGAKITEQGVKAFYRPATDEIVLP
                     ERFRFADAANFYATGLHELVHWTGAASRLNREKGNKFGSEAYAFEELIAELGSAFLLA
                     DLGITGEVQHESYIASWLKALKGDKRYIFKAAAAASKAHRWLMDC"
BASE COUNT         1362 a         1506 c         1559 g         1283 t
ORIGIN      
        1 gcatcgtcgg cacattatta gttagggaca acggttcaat caatgcctgt cacgaacagg
       61 tattggcttg tcgccagcaa cccgttgagc ggagtaatca cgcagcagga cggctgttac
      121 aggcgatggt gaaagctctg ttgggatctg aacgaagcat gttgagtgaa atttgcaaaa
      181 tttcgcagag tatgcggctt catctttaaa tttccgccag catttttaaa gccagctacc
      241 gacgctgcgc gttggccggc ccttcgtccg gcgggtaacc agtaccggcc gatcttacga
      301 ccctggatga acatcatccc gtcagcagcg tctccactgt gatgtcgttt ttcacactgt
      361 ccgatcacaa aatagaacgg gcagggacaa catatggtag gaaaagcagc tgccagagac
      421 acaatatatt gattacccac agtaacgggg gataaccatg tgggtaacat attgaaagtg
      481 caacacgtta tctgattaat tgactttgta aacgccgctg aaccatgaaa aaaaaccggg
      541 ggaatcatca cagcgcataa acataccgtt ttgtttttgg gtgacggcaa ggaggcccct
      601 tttatcacca cataccttgt caaaggtttc catttctatc cttatcaggc tcttgtcgtt
      661 attttcagga gagatccgga gatgttttgt cagccagaca ggataggtat cagcgttacg
      721 ccagtactgc aattcaatca ctgaagccat tccccagaca gcgaagtata agaaaaccac
      781 acctgcgatt ttgctaccca tattgttcct taacattggc ttaatacatt ccttgctcct
      841 cgcaccggag cacatcacag atactgattg agatccactt cacgcagcgg ctggttttga
      901 cgttctgtta tcgtttgcac caggtctttt tcgtccagct catcgagcat ttgttcatac
      961 aaagccgcgg gcacacaata aaaaacaggg cggttacggt caattatctc gaccgggtag
     1021 ccgccgcggg cactggcggt ggccaccgga tcgtttttaa gttcgctgac gctggcagtc
     1081 acatcacaca gaatgatatt tgtcatggtt cccccctgag acaagtcacc tgtatgatcg
     1141 gtaaagtaag ctctggcggc aagtcccttc tgtcatttca acggtcagaa acgggcaacc
     1201 ggcagctcct tccagcgtgt ggtatatgcc ggtgacagca tctcccgttt catctgccag
     1261 tccggcgcaa taccccgtcc ggcaaaccag actttcccga gtccggaatg attaatgccg
     1321 tcgagtacct tcatcagcgc atcgctgtgc ggccgtggct gcacgtcatc gaacagattc
     1381 agctgcgcga cgccgttcgg actgaaatca ttgagcatta tccccgcttt tgcgtagcgg
     1441 tggccatcga gccatatcct gtccagagac ctgaccgctg ccgcgataat gtcccgggtg
     1501 tcacgggtag gggtgttcag tttttccgtg gccacgttac cgtaatacgg ctcattaacc
     1561 gcaaaagggg atgtcttgat gaaggtgctg atatgccggc agtactgtcg ctcaccgcgc
     1621 aacttttccg ccgcgcgctc ggcatactgg cagacggcct ggcgcatttc tccgtaggtg
     1681 gtgatccgct gcccgaagct gcgactgcag acaatctgct gtttcggcgg cggcgcttcc
     1741 tccagcgaaa tgcaactctc gccgttcagc tcacgtaccg tgcgctccag cactacgttg
     1801 aagtttttcc ggataaatgt agggttcgtc agcgacagct gcagggcggt ggtgatcccc
     1861 ataacgtgca gctttttcgc tatccggttc cccacgcccc agatttcttc caccggctgc
     1921 aggctgagta attttgccgt tctgcgtgga ttttcgggcg acagcgccag caccccactg
     1981 aattgtggcc attcctttga cgcccactgc gccgatttgg ccagtgtttt tgtggcgccg
     2041 aagccgacac cgatcgtcag tccggttcca ctgagaacat gcccacgcag ctgcctgcca
     2101 aaatcctcca gatccataca gtggctaatt ccccgggcat ccaggaaaca ttcgtcaatt
     2161 gagtattgtt cgacgcgggg cgacagctct tccagcaggg cgaccacacg gttactcagg
     2221 ctcgcgtaga gctcgtagtt gctggaaaaa acgtacagtt tttcaggaaa ctgcgcctct
     2281 ttgagctgga accagggggt tcccatcttt atacccagcc gtttggcttc agcactccgg
     2341 gcaataacgc agccatcgtt gttactgaga acgacgacag gtttgccgcg caaatccggc
     2401 ctgaaaacct tctcgcaact ggcgtagaag gagttgacgt ctgccagggc gaacatcagt
     2461 ctgccctccg ggttttatga atgaaggccg tcaccacgcc gaaaatttgc agagtttccg
     2521 gatccgggta cagggaaggg aagtccgggt tcatcgcctg cagggctggc cgcggcgtca
     2581 gaagcaggcg ttttaccgta aattcaccct caatttccgc gataacaata tccccgtgcc
     2641 gcggctgttc tgctttatcc acgaccatca gatcgccgga atagaggcca atatcagtca
     2701 ttgagttgcc aatcgctctg acaaaatagg tggagtggcg ccggtgaatg cagtactcat
     2761 tcagatccag ttcagactca gtatagtttg ctgccggtga cggaaaaccc gcagggcatc
     2821 gctctgtgaa caaaggaatt tttactatgc tggcttctga tactgcagga atgaacttca
     2881 tcgttttttc cctcgcttaa aactgtatat gcatgggtct tcccttgttg tggtggctga
     2941 aggcatgata atggtgtatt taatcgccag aggtcaccgc catggacgaa aagtccctct
     3001 acgctcatat tctcaacctg tccgatccgt ggcaggtaaa gtccctttct ctcgatgaaa
     3061 atgccggttc tgttactgtc actattgaga tcgctgaaaa cacccggcta gcctgtccga
     3121 cctgcggtaa atcctgttct gttcacgatc accgtcatcg taaatggcgc catcttgata
     3181 cctgccagtt caccactatt gttgaagccg atgttccacg aattatgtgt ccggagcatg
     3241 gctgcctgac gttgcctgtt ccgtgggctg gccccggaag ccggtatacg ttgctattcg
     3301 aatcgttcgt tctctcatgg ctgaaaatca gcaccgttga tgctgtcagg aagcaactta
     3361 agctcagttg gaatgcggtt gacggcatta tgacccgggc agttaagcga ggtcttgccc
     3421 ggataaaaaa gccattatcc gcccgtcata tgaatgtgga tgaggtcgcc tttaaaaaag
     3481 gacatcgtta cataacggtg atctccgatc gcgatggtcg ggcgctggcc ttaacggatg
     3541 atcgcggcac agagagtctt gccggctatc ttcgcacgct cactgatggg cagttgctgg
     3601 ctatcaaaac gctctcaatg gacatgaacg cgggctatat aagagcagcg cgtatccact
     3661 tacccagtgc ggttgagaaa atcgcctttg accgcttcca tgtggcgaag caactgggcg
     3721 aggtagttga taaaacccgt cagaatgaac atccgcacct ccctgttgaa agccgacacc
     3781 aggcaaaagg aacccgcttc ctgtggcagt acagcgataa gtggatgacc gaatcccggc
     3841 aggaaaagct gatgtggctg cgtgcacaga tgaagctgac gagccagtgc tgggcgctga
     3901 aagagctggc aaaggatatc tggaacaggc catggagcga ggaaagacgg agtgactggc
     3961 agagatggtt ggcgctggcg gctaacagtg acgttcccat gatgaaaaat gccgcgaaaa
     4021 cgataggaaa aaggctgtag gggatcctga atgcgatgcg acacagtgtc tcaaacggaa
     4081 atgcggaggc acttaacagc aagatcaggc tgctgaggat aaaagccagg ggataccgaa
     4141 accgggagcg ctttaaactg ggggtgatgt tccactacgg aaagctgaat atggcgttct
     4201 gagccttccc accatgatcg gggaagaccc atatgcatac agtataaggg gcggtgaaat
     4261 ttctgcaagt gatgggggga tagccggtca gaacgtcccc gaaaacaaac cgcccccggt
     4321 ttcaggaagg gcgaactgac cggcagaggc agtgtaacac cgcatgtcac aaaggcagca
     4381 ctgacgtcgt cagtgctttt tttacccgtc ctcccggacg ctgaagggca gtttcacccg
     4441 tgcagctaaa ggggcggtaa gtccgggtgt gcacatcgtc attttctcct gtgatgcctt
     4501 tctcctgcgt atcagcttcc ggtaatcgtc ccccgtcaca acctggacag caagggtaaa
     4561 tggcacgctg cgcgcccttg ctgaccagaa cgactccggg caggccgaac cgtcaggcga
     4621 tacgaagacg aaattcacac tgacggagaa acagccatga cgatgaacct gcacactcag
     4681 acaaccgccc ctaaagcttc acaggcgacc agcgtatccc cgctggagca gtcaggctcc
     4741 tcaaaaacga aattttccag gggcaaaagc aaagcccgtg ctggcgcagt caaaacagac
     4801 ctttaccaga cagtaacgga cagcatcatt gcagccctgg aaagcggcgt taagccgtgg
     4861 gtgtgtccgt gggttcgcaa cggcgcagcg gtgggattac cggccaactt cagcaccggc
     4921 acggcataca gcggaattaa tatcatgctg ctgtggtgta gtgcggcaaa acagggtttt
     4981 caggacgagc gctggctgac ctacaaacag gcgcaggagc tgggcggcca ggttcgtaaa
     5041 ggtgaacacg gcacgacggc catcttttac aagacgctgg agaaagagga cgaggacggg
     5101 gaaatcgaaa aaatcccgat gctgaaagcc tttaccgtgt ttaacgtgga gcaaatcgac
     5161 ggcctgagca tcgagagcgt gccgcagccg gttgccggtt tcgatccgct gccgcaggcg
     5221 gaagcactca tgatccggag cggggcaaaa atcaccgagc agggcgtaaa ggcgttctat
     5281 cgtccggcca ccgatgaaat cgtcctgccg gagcggttcc gcttcgcgga cgcggcgaac
     5341 ttctacgcca ccggcctgca tgagctggtt cactggacgg gtgcagcgtc ccgcctcaat
     5401 cgtgaaaaag gcaataagtt tggctctgag gcgtatgcgt ttgaggaact gatcgccgag
     5461 ctgggcagcg cgtttctgtt ggcggatttg gggattacgg gagaggtgca gcatgaaagc
     5521 tatatcgcgt cctggctgaa agcgctgaag ggagacaagc gctacatctt taaagcggcg
     5581 gcagcggctt caaaagcgca ccgctggctg atggactgct gacagctgga gccctgcggt
     5641 gcagggcttc agaatttttg aaagagagag aatcagggag ggaattatat aagtcactgt
     5701 tttttagaat
//
LOCUS       DADLSB010000209         5697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.209, whole genome shotgun sequence.
ACCESSION   DADLSB010000209 DADLSB010000000
VERSION     DADLSB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.209"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            67..282
                     /gene="xisR"
                     /locus_tag="JN331_003794"
     CDS             67..282
                     /gene="xisR"
                     /locus_tag="JN331_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HAY7261792.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            284..1519
                     /locus_tag="JN331_003795"
     CDS             284..1519
                     /locus_tag="JN331_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7261793.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVS
                     SLGARWNAAVKRAGIRRHNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            2006..2896
                     /gene="sitA"
                     /locus_tag="JN331_003796"
     CDS             2006..2896
                     /gene="sitA"
                     /locus_tag="JN331_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY7261794.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDNPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            2896..3723
                     /gene="sitB"
                     /locus_tag="JN331_003797"
     CDS             2896..3723
                     /gene="sitB"
                     /locus_tag="JN331_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY7261795.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            3720..4577
                     /gene="sitC"
                     /locus_tag="JN331_003798"
     CDS             3720..4577
                     /gene="sitC"
                     /locus_tag="JN331_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY7261796.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            4574..5431
                     /gene="sitD"
                     /locus_tag="JN331_003799"
     CDS             4574..5431
                     /gene="sitD"
                     /locus_tag="JN331_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY7261797.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         1517 a         1305 c         1373 g         1502 t
ORIGIN      
        1 cctcattaat aatcaacatc gggcgtcaat ttcagcctaa cattggcgcc tgccagaggt
       61 gatgcgatgg cacaagtaat ctttaatgaa gagtggatgg ttgaatacgg cctgatgctt
      121 cgcactggtc tgggtgccag acaaattgaa gcataccgcc agaactgttg ggtggaaggc
      181 ttccacttca aacgagtatc tcctttaggg aagccagaca gtaagcgagg gattatctgg
      241 tacaactatc cgaagataaa tcagtttatc aaagactcat gatatgtcta aattaccaac
      301 aggtgtcgag attcgaggta aatacattcg catctggttc atgtttcgag gaaaacgatg
      361 tcgggaaaca ttgaaaggct gggagattac taacagtaac attaaaaagg ccgggaattt
      421 aagagcgttg atagttcatg aaatcagttc cggtgaattt gagtatttaa gacgttttcc
      481 ccagtccagc actggggcta aaatggtgac aacgagggtc ataaaaacat tcggggagct
      541 ttgtgatatc tggacaaaaa ttaaagagac agagttaaca acaaacacaa tgaagaaaac
      601 gaaatcacaa ttaaaaacac tcaggataat aatttgtgag agtaccccga tatcgcatat
      661 tcgttatagc gatatcttaa actaccggaa tgaactgctg catggagaaa cgctttacct
      721 agataatcca agatccaaca aaaaaggaag aaccgtgcgc acagttgata actatatcgc
      781 cctgctctgt tcgttgttac gttttgcgta tcagtcggga tttatatcaa ccaaaccatt
      841 tgaaggagta aaaaaattac agcgaaacag aataaagcct gacccgttat ctaaaacaga
      901 attcaatgca ttaatggaaa gtgaaaaagg acagagccaa aacttgtgga aatttgccgt
      961 ttactccggg cttcgtcacg gggaactggc agctctggcg tgggaggatg tggatttcga
     1021 gaagggaatt gtgaatgtca gaagaaacct gacgatactt gatatgttcg gtcccccaaa
     1081 aacaaatgcc gggatccgga cggtaacatt actgcagccg gctcttgaag cactgaagga
     1141 gcaatacaaa ctgaccgggc atcatcgcaa aagcgaaatc actttttatc atcgggagta
     1201 cggcagaact gaaaagcaaa aactgcattt tgttttcatg cccagggtgt gtaacgaaaa
     1261 acagaaacct tattactcgg taagcagttt gggtgcaaga tggaatgcag cagtaaaacg
     1321 tgctggtatt cgccgccata atccgtacca tacgcgacat acttttgcct gctggctgtt
     1381 gacggcagga gcgaacccgg catttatagc cagccagatg gggcatgaaa ctgcgcaaat
     1441 ggtgtatgaa atttacggta tgtggattga tgacatgaac gacgaacaga tagccatgtt
     1501 gaatgcgcgg ttatcgtagt tgcaaagttt gcccccaatt tgccccattt agtaccagag
     1561 aactgaaata atgcaagaaa atcaacaaat tacaaagaaa gaacaataca acctgaacaa
     1621 gtaagggcaa aaatcacaac tatctgactc ataagtattt tatttatttt tcaatatgtt
     1681 aaacattatc agcgacaaca ataagctacg ataacccact ctttttttgc cccttttttg
     1741 ccccttctac agcgttttgc cccatttttg ccatcaacaa ttttcctcat aaagtcattt
     1801 caaagcctaa tagctgctct acttttttcc tcaatatcat gtcgccagca ggaaacaacc
     1861 taatccctgt tttaggcctc aaagctgtta atgataatta ttttcatttt gtataggtta
     1921 tgctataaaa catagccgtt gctatgtttg ttctaatttt gctcactata ggtactaaat
     1981 tatgcactca ataaaaaaag taaccatgct cttggggtgc ctcgccctca cctgctcgat
     2041 cgcatttcag gcaagtgcaa ctgaaaaatt caaggtcatt acaacattta ccatcatcgc
     2101 ggatatggcc aaaaacgtgg ctggagatgc tgcagaagtc tcatccataa ccaagcctgg
     2161 tgcagaaatt catgagtatc agcctacccc tggcgatatt aaacgtgcgc agggggcaca
     2221 actgattctc gccaatggta tgaatctgga attgtggttc caacgctttt accagcatct
     2281 caatggggtt ccagaagtaa ttgtctcttc gggtgtgacg ccagtaggga tcaccgaagg
     2341 accctatgag ggcaaaccta atccccatgc atggatgtcg ccagataatg ctctgattta
     2401 cgtcgataat attcgtgatg cgttgataaa atacgatccg gcaaatgcac aaacctacca
     2461 acgtaatgcc gatacttata aagccaagat tacccaaacc cttgcccccc tgcgtaagca
     2521 gattacggaa ctccctgaga atcagcgatg gatggtcacc agtgaagggg ctttttctta
     2581 tctcgcacgc gatttggggc taaaagagct ttatctgtgg ccgattaatg ccgatcaaca
     2641 aggaacaccg cagcaggtac gtaaggttgt tgatatagtt aagaaaaatc atataccggc
     2701 agtctttagc gagagtacga tttccgataa tccagcgcgt caggttgcgc gtgaaaccgg
     2761 cgcacactac ggcggtgtgc tctatgtcga ttccctgagc acagaaaacg gcccggtacc
     2821 tacgtatatc gaccttctta aggttactac cagtacgctg gtacagggaa ttaaagccgg
     2881 gaaaagggag aaataatgat gcaatctgca ggcattgtcg ttaatgatgt tactgtcacc
     2941 tggcgtaacg ggcacacagc actgcgcgat gcatccttca cagtaccaag tggatctatt
     3001 gccgctctgg ttggggtaaa cggttccgga aaatcgacgt tgtttaaagc gattatggga
     3061 tttgtgcgtc tgaccagcgg aaagatatct gttctgggta ttcccacgcg acaggcgcta
     3121 cagaaaaacc tggttgccta cgtccctcag tcagaagagg ttgactggtc atttcctgta
     3181 ctggtggaag atgtggtgat gatgggccgc tatgggcata tgggtatgtt gcgtatagcc
     3241 aaaaagaggg atcgtcagat tgtcaccgat gcgctggaac gtgtcgatat ggtggatttt
     3301 cgccatcgtc aaatcggcga gctttccggt ggacaaaaga aacgcgtctt tctggcgaga
     3361 gcgattgcac agcagggtga tgtgatcctg ctggatgaac cttttaccgg cgttgatgtg
     3421 aaaaccgaag cgaaaatcat cagcctgttg agggagttac gcgcggaagg caaaacgatg
     3481 cttgtctcaa cgcacaatct cggctcagtc acgacatttt gcgattatac ggtcatggtc
     3541 aaaggcaccg tcctggcaag cgggccgaca gacaccactt ttacagccga gaacctggag
     3601 ctggctttta gcggcgtact gcgccatgtc acacttaacg gctcggaaga aagcattatt
     3661 actgacgatg aacgcccttt tgtggcacat cgaccgtcag cggtgcagag ggaagcaaga
     3721 tgaacgtgct actggaaccc ttcagctatg aatatatgct caatgcgatg tgggtctcgg
     3781 caatggtcgg cggtctctgc gctttcctgt catgctatct gatgctaaaa ggctggtcgc
     3841 tgattggcga tgcgctgtcg cactctattg tcccgggtgt tgcgggggca tatatgctgg
     3901 ggcttccgtt ttctctggga gccttctttt ccggcggact ggcagcgggc agcatgctgt
     3961 ttcttaacca acgcacccgc ttaaaagaag atgccatcat cggcctgatc ttctcgtcct
     4021 tttttggcct ggggctgttt atggtgtcat tgaacccgac atccgtgaac attcagacca
     4081 tcgtcctggg caatattctg gcaatcgatc cggcggatat cctgcaactc actatcattg
     4141 gcattctctc gatcatcgtg ctttttttca aatggaaaga tcttatggtg acctttttcg
     4201 acgagaatca cgcccgtgct attggattac atccgggcag attaaaaatc ctcttcttca
     4261 ccctgctgtc ggtttcgacc gttgcggcgc tgcaaacggt tggtgccttt ctggtgatct
     4321 gtctggtggt gactcccggt gcaacagcat ggttgttaac cgatcgtttt ccccgcctgc
     4381 tcataattgc tgtaactatt ggcagtgtaa ccagtttcct cggcgcgtgg gtcagttact
     4441 ttctggacgg cgcgacaggt ggaatcatcg tggtggcgca gacactgctg tttcttttag
     4501 catttgtatt tgcgcccacc catggacttt tggcaaatcg tcggcgtgcg cataaagcgc
     4561 tggaggatcg ctcatgatgg cattattact ggagccactg cagttcacct ttatgagtca
     4621 cgcactgctg atttcactgg tagtttccat accctgtgcg ctgctgtcgg tctttctggt
     4681 cctgaaaggt tgggcactga tgggtgacgc gatgagtcat gcggtttttc ctggcatcgt
     4741 gcttacctgg attctggggt tacctctggc tacaggggca ttcgtcgccg gagtattctg
     4801 cgcggtggcg acgggatacc tgaaagacaa cagccgaatc aagcaagata cagttatggg
     4861 gattgttttt tctggcatgt ttgccgcagg tctgatcttg tatattgcaa ttaaaccaga
     4921 cgtacatctt gaccatattc ttttcggcga catgctcggg ataaccatcg gcgatataat
     4981 ccagacggtg attattgccg ggctggttac acttgttatt agcgtaaaat ggcgggattt
     5041 tttgctgttc agctttgatt atcaacaggc gcaggtaagc ggtttgcata cgaggtggct
     5101 gcattatggg ctgctgtgca tggtttccct gaccattgtg gcgacgctga aagcagtagg
     5161 tatcattctc tctatttctc tgctcattgc gcctggcgcc attgcagtac tcctgacaca
     5221 acgctttcat attgcgctcc tactggcgac tggcatatcg gtaatagtgt caatgactgg
     5281 tgtctggctg tcctttttta tcgacagcgc accagctccg acgattgtcg tcttattcgc
     5341 ggtcgtgttc atcatgacgt ttaccgttac cagcatcaac gcacgcacaa agggaaacgc
     5401 ccatacacag gatctgttat cacccaatta gcacaacatc cgaggacagc atcgtccgcc
     5461 agcctacaga agtccggaaa gccatggaag gtcgttggga ggaggtacga attctatcat
     5521 gcaaaaaata cgtaaaatcg ataacgccta gaaatcattc aatactcgca ctatcgaaag
     5581 tttaccagcc aaccgcggca cgttcctgca tacgacgtgc cgcagcttct gttattccac
     5641 gataaaattc ctaaccgaat tcaaatcctt cagactattc agctcttcct tcagctc
//
LOCUS       DADLSB010000210         5688 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.210, whole genome shotgun sequence.
ACCESSION   DADLSB010000210 DADLSB010000000
VERSION     DADLSB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.210"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            142..1794
                     /locus_tag="JN331_003800"
                     /pseudo
     CDS             142..1794
                     /locus_tag="JN331_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
     gene            1959..2678
                     /locus_tag="JN331_003801"
     CDS             1959..2678
                     /locus_tag="JN331_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY7261798.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNEREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            2724..3275
                     /gene="fliZ"
                     /locus_tag="JN331_003802"
     CDS             2724..3275
                     /gene="fliZ"
                     /locus_tag="JN331_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY7261799.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            3363..4163
                     /gene="tcyJ"
                     /locus_tag="JN331_003803"
     CDS             3363..4163
                     /gene="tcyJ"
                     /locus_tag="JN331_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7261800.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            4268..5254
                     /gene="dcyD"
                     /locus_tag="JN331_003804"
     CDS             4268..5254
                     /gene="dcyD"
                     /locus_tag="JN331_003804"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY7261801.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            5269..>5688
                     /locus_tag="JN331_003805"
     CDS             5269..>5688
                     /locus_tag="JN331_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7261802.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQ"
BASE COUNT         1482 a         1362 c         1477 g         1367 t
ORIGIN      
        1 gtcgactaac aaaaaatggc tgtttgtgaa aaaaattcta aaggttgttt tacgacagac
       61 gataacaggg ttgacggcga ttgagccgac gggtggaaac ccaaaacgta atcaacgact
      121 tgcaatatag gataacgaat catggcacaa gtcattaata ccaacagcct ctcgctgatc
      181 actcaaaata atatcaacaa gaaccagtct gcgctgtcga gttctatcga gcgtctgtct
      241 tctggcttgc gtattaacag cgcgaaggat gacgccgcgg gtcaggcgat tgctaaccgt
      301 tttacttcta acattaaagg cctgactcag gctgcacgta acgccaacga cggtatttct
      361 gttgcacaga ccactgaagg cgcgctgtcc gaaatcaaca acaacttaca gcgtatccgt
      421 gagctgacgg ttcaggcttc taccgggact aactctgatt cggatctgga ctccattcag
      481 gacgaaatca aatcccgtct cgacgaaatt gaccgcgtat ccggtcagac ccagttcaac
      541 ggcgtgaacg tactggcaaa agacggttcg atgaaaattc aggttggtgc gaatgacggc
      601 cagactatca ctattgatct gaagaaaatt gactctgata cgctggggct gaatgggttt
      661 aatgtgaatg gtggcggggc tgtggctaat actgctgcat ctaaagctga cttggtagct
      721 gctaatgcaa cagtggtcgg caacaaatat actgtgagtg cgggttacga tgctgctaaa
      781 gcgtctgatt tgctggctgg agttagtgat ggtgatactg ttcaggcaac cattaataac
      841 ggcttcggaa cggcggctag tgcaacgaat tacaagtatg acagtgcaag taagtcatac
      901 tcttttgata ccacaacggc ttcagctgcc gatgttcaga aatatttgac cccgggagtt
      961 ggtgataccg ctaagggcac tattactatc gatggttctg cacaggatgt tcagatcagc
     1021 agtgatggta aaattacggc aagcaatgga gataaactct acattgatac aactgggcgc
     1081 ttaacgaaaa acggctctgg tgcttctttg actgaggcta gtctgtccac acttgcagcc
     1141 aataatacca aagcgacaac cattgacatt ggcggtacct ctatctcctt taccggtaat
     1201 agtactacgc cggacactat tacttattca gtaacaggtg caaaagttga ttaggcagct
     1261 ttcgataaag ctgtatcaac ctcgggaaac aatgttgatt tcactactgc aggttatagc
     1321 gtcaacggca caactggcgc tgtaacaaaa ggtgttgatt cggtttatgt tgataacaac
     1381 ggggcgttga ccacatctga tactgtagat ttttacctac aggatgatgg ttcagtgact
     1441 aacggcagcg gtaaggcagt ttataaagat gctgatggta aattgacgac agatgctgaa
     1501 acaaaagcag ctacgacagc cgatcccctg aaagctctgg atgaagccat cagctccatc
     1561 gacaaattcc gctcctccct gggtgcggtg caaaaccgtt tggattccgc agtcaccaac
     1621 ctgaacaaca ccactaccaa cctgtctgaa gcgcagtccc gtattcagga cgccgactat
     1681 gcgaccgaag tgtccaacat gtcgaaagcg cagattatcc agcaggccgg taactccgtg
     1741 ctggcaaaag ccaaccaggt accgcagcag gttctgtctc tgctgcaggg ttaatcgtct
     1801 gaatgcctct caaaccccgc caccggcggg gttttttaca ttcggctttt tgcgtaaccc
     1861 ccaaataacc cctcatttca cccactaatc gtccgattaa aaaccctgca gaaacggata
     1921 atcatgccga taactcatat aacgcagggc tgtttatcgt gaattcactc tataccgctg
     1981 aaggtgtaat ggataaacac tcgctgtggc agcgttatgt cccgctggtg cgtcacgaag
     2041 cattgcgcct gcaggttcga ctgcccgcga gcgtggaact tgacgatctg ctacaggcgg
     2101 gcggcattgg gttacttaat gctgtcgaac gctatgacgc cctacaagga acggcattta
     2161 caacttacgc agtgcagcgt atccgtggcg ctatgctgga tgaacttcgc agccgtgact
     2221 gggtgccgcg cagcgtgcga cgcaacgagc gtgaagtggc acaggcaata gggcaactgg
     2281 agcaggaact tggccgcaac gccacggaaa ctgaggtagc ggaacgttta gggatcgata
     2341 ttgccgatta tcgtcaaatg ctgctcgaca ccaataacag ccagctcttc tcctacgatg
     2401 agtggcgtga agagcacggc gatagcatcg aactggttac tgatgatcat cagcgagaaa
     2461 acccgctaca acaactgctg gacagtaatc tgcgccagcg ggtgatggaa gccatcgaaa
     2521 cgttgccgga gcgcgaaaaa ctggtattaa ccctctatta ccaggaagag ctgaatctca
     2581 aagagattgg cgcggtgctg gaggtcgggg aatcgcgggt cagtcagtta cacagccagg
     2641 ccattaaacg gttacgcact aaactgggta agttataacg tcagtaaatg ccgcacttta
     2701 actttgacta ccaggagttc ttaatgatgg tgcagcacct gaaaagacgg ccattaagcc
     2761 gctatcttaa agactttaaa cacagccaga cccattgcgc gcattgccgt aaattactcg
     2821 atcgcattac cttagttcgc gacggcaaaa tagtgaataa aatcgagatt tcccgcctgg
     2881 acacgctgct tgatgaaaaa ggctggcaaa cggaacaaca atcatgggcg gcattgtgcc
     2941 gattttgcgg tgatttacat tgcaaaacgc agagtgattt tttcgatatt atcggcttta
     3001 agaaatttct ttttgagcaa actgaaatga gcccaggtac ggtgcgtgaa tatgtcgttc
     3061 gtctgcgccg tttgggtaat catctacatg agcaaaatat ttccctcgat cagctgcagg
     3121 acggtttcct tgatgaaatc ctcgccccgt ggctgcccac caccagcacc aacaattacc
     3181 gcatcgcatt acggaagtat caacactatc agcgccaaac ctgtaccgga cttgtgcaga
     3241 aatcctccag cctgcctgct tctgatatat attaaataag aataagatgt agcggagttg
     3301 tttttgtgtt tacaaacaat ggctctacac tgcaaacaga cataacaaca ttcggggtga
     3361 atatgaaatt agcacatctg ggacgtcagg cattgatggg tgtgatggcc gtggcgctgg
     3421 ttgcgggcat gagcgttaaa agttttgcag atgaaggtct gcttaataaa gtcaaagagc
     3481 gcggcacgct gctggtaggg ctggaaggca cttatccgcc gttcagtttt cagggggatg
     3541 acggcaaatt aaccggtttt gaagtggaat ttgcccaaca gctggcaaaa catcttggcg
     3601 ttgaggcgtc actaaaaccg accaaatggg acggtatgct ggcgtcgctg gactctaaac
     3661 gcattgatgt ggtgattaat caggtcacca tttctgacga gcgcaagaaa aaatacgatt
     3721 tctctacccc ttacaccatt tccggtattc aggcgctggt gaaaaaaggt aacgaaggca
     3781 ccattaaaac ggctgctgat ctgaaaggca aaaaagtggg tgtcggtctg ggcaccaact
     3841 atgaagagtg gctgcggcag aatgttcagg gtgtcgatgt gcgtacctat gatgatgacc
     3901 cgaccaaata tcaggatctg cgcgtagggc gtatcgatgc gatcctcgtt gatcgtctgg
     3961 cggcgctgga tctggtgaag aaaaccaacg atacgctggc agtaaccgga gaagcattct
     4021 cccgtcagga gtctggcgtg gcgctgcgta aaggaaatga agacctgctg aaagcggtga
     4081 atgatgcaat tgcggaaatg caaaaagacg gcactctgca agctctttcc gaaaaatggt
     4141 ttggtgctga tgtgaccaaa taatcagcat aatgacaaaa aagggcgctt tcactagcgc
     4201 cttttttatt tacgcgtttt cagcgtgcat aataagaaaa tcacaacaac acacatcgga
     4261 ggctgttatg ccactgcata atttaacccg ttttccacgg ttggagttta tcggcgcgcc
     4321 aacgccgctc gaatatctgc cgcgcttttc tgattatctt ggacgggaaa ttttcatcaa
     4381 acgggatgac gtcaccccca tggcaatggg cggcaataaa ttacgtaagc tggaatttct
     4441 cgcggcagat gccctgcgcg aaggtgccga tacgctgatt actgccggcg cgatccagtc
     4501 taaccatgtg cgccagactg ccgcagtcgc ggcgaaactc ggtctgcact gcgtggcgct
     4561 gctggaaaat cctattggca caaccgcaga aaactattta accaacggca atcgtttgtt
     4621 gctggatctg ttcaataccc agattgaaat gtgcgacgca ctgaccgatc ccaatgccca
     4681 actggaagag ctggcgacgc gagtcgaagc acaaggcttt cgcccgtatg tcattccggt
     4741 tggcggttct aatgctcttg gcgcgctagg ttatgtggag agtgcgctgg aaatcgcgca
     4801 acagtgtgaa ggggcggtta atatttcgtc ggtggtagtc gcatcgggca gtgccggaac
     4861 tcacgccgga ctggctgttg ggctggaaca cctgatgcct gaaagcgaac tgattggcgt
     4921 gaccgtgtcg cgttccgttg ccgatcaatt gccgaaagtg gttaacctac aacaggcgat
     4981 tgcgaaagaa ctggagctga ccgcatcagc ggaaatttta ctctgggatg actattttgc
     5041 acctggctac ggcgtgccga acgacgaagg catggaagca gtgaaattgc tggcgcgtct
     5101 ggaaggcatt ctgcttgatc ctgtgtatac cggaaaagcg atggcggggc tgattgacgg
     5161 tatcagtcag aaacgcttca aagatgaagg gccgattctg tttattcata ccggcggcgc
     5221 gcctgcgctg ttcgcctatc atccccacgt ttagctccgg gaacgctcat gcaagaaagt
     5281 atacaactgg ttattgattc cttgccgttc ctgttgaaag gggctgggta taccctgcaa
     5341 ctcagtatcg gcggcatgtt ttttggctta ctactcgggt ttattctcgc gctgatgcgc
     5401 ctgtcgccaa tctggcccgt gcgctggctg gcgcgttttt atatctctat ttttcgtggt
     5461 acgccgctca tcgcacaact gtttatgatc tattacggcc tgccgcagtt tggtatcgaa
     5521 ttagatccca ttccgtcagc aatgattggt ctgtcgctga acactgccgc ctatgccgcc
     5581 gaaacgctgc gcgccgccat ttcttctatt gataaaggtc agtgggaagc cgctgccagt
     5641 attggtatga cgccgtggca gaccatgcgt cgcgcgattt tgccacag
//
LOCUS       DADLSB010000211         5686 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.211, whole genome shotgun sequence.
ACCESSION   DADLSB010000211 DADLSB010000000
VERSION     DADLSB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.211"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            64..972
                     /locus_tag="JN331_003806"
     CDS             64..972
                     /locus_tag="JN331_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY7261803.1"
                     /translation="MKAPVSLTSFHPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1018..3009)
                     /gene="tkt"
                     /locus_tag="JN331_003807"
     CDS             complement(1018..3009)
                     /gene="tkt"
                     /locus_tag="JN331_003807"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7261804.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3287..4045
                     /locus_tag="JN331_003808"
     CDS             3287..4045
                     /locus_tag="JN331_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7261805.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVAPGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4251..5171)
                     /gene="speB"
                     /locus_tag="JN331_003809"
     CDS             complement(4251..5171)
                     /gene="speB"
                     /locus_tag="JN331_003809"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY7261806.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5307..>5686)
                     /locus_tag="JN331_003810"
     CDS             complement(5307..>5686)
                     /locus_tag="JN331_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261807.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYIVN
                     AGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1364 a         1521 c         1512 g         1289 t
ORIGIN      
        1 tgtcggctgt tttctcctca tctcatgtga catattccca tactttactc aaggaagatt
       61 gtcatgaaag cacctgtttc attaacctca tttcacccac aaaaatctct tgctatcgcc
      121 attggcgtgc ttgccgtggt tgtgctgcca tttctctcgt attacaccgt taatgaaggt
      181 gaacgcggca ttttgctgcg ctacggaaaa atcgtaaaag ttgccgagcc ggggctgggt
      241 tttaagatcc cttttatgga aagcgtggag aaaatttcca cgcgtaatca ggctgtggtc
      301 tatcagggat tacaggccta cagccgcgac cagcaaccag cacaaatgac agtatcagtc
      361 agcttccaca ttaaaccttc agaagcaggc gcggtttata ccacctacaa cactattgaa
      421 gcattaaaag atcgtctaat tgtccgtcag ttgccaactc agctggagaa cattttcggc
      481 caatataccg ccatcagcgc cgtacaggat cgtacaaaat tggttcagga tctgcaaaat
      541 gccatgcgca aagccgttgt tgggccagtg gtaattgacg gggtccagat tgaaaatatt
      601 gatttttctg acgcttatga aaagtcgatt gaagaccgaa tgaaagcaga agtcgccatt
      661 gccacgcgta agcaaaatct ggaaacagag aaaatccagg cgcaaatagc ggtaactcag
      721 gcccaggcag aagcagacag taaactggcg gcagccaaag cagaagcgga aactattcgc
      781 gtaagaggcg cggcagaggc tgaaactatt cgtttaaaaa gtgcggctga agcagaagca
      841 atccgcctgc gcggtgaagc tctgcgggat aatcccggac ttgtagccct caccactgcc
      901 gaacgttggg acggtaaact gcccgatacc atgatccccg gcagcacagt tccgtttatc
      961 tctactaagt aaaaaaaggg ccgctttcgc ggcccttctc acaaaacgtc ttactgatta
     1021 cagcagcgct tttgctttcg caacaacgtt atcaacagtg aagccgaact cttcaaacag
     1081 cagctctgcc ggagcagatt caccgaaggt ggtcatgccg acgatagcgc cgttcaggcc
     1141 aacatacttg taccagtagt cagcaatacc cgcttctacc gcaacgcgtg cagtaaccgc
     1201 tttcggcagt acggattcac ggtaagccgc atcctgcttg tcaaatgcgt cggtagacgg
     1261 catggacacc acgcgcgctt tcacgccttc ggcagtcagt ttttcgtagg cagcaaccgc
     1321 cagttcaact tcagaaccgg tggcgatgaa gatcagttcc ggctgaccag cacagtcttt
     1381 cagcacataa ccaccgcgcg cgatgtttgc cagctgctct tcagtacgtt cctgctgcgc
     1441 caggttctga cgggaaagga taagcgcagt cgggccgtcc tgacgctcaa cgccgtattt
     1501 ccacgcgacc gcggattcaa cctggtcaca cggacgccat gtagacatgt tcggggtcac
     1561 gcgcagagaa gcgacctgct ctaccggctg gtgagtcggg ccatcttcgc ccagaccgat
     1621 ggagtcgtgg gtgtaaacca tcacctggcg ctgtttcatc agcgcagcca tacgtacggc
     1681 gttacgtgcg tattccacga acatcaggaa ggtggaggtg tacggcagga agccaccgtg
     1741 cagggagata ccgttagcaa tcgcggtcat accgaactcg cgaacaccgt agtggatgta
     1801 gttacctgca gcatcttcgt tgattgcttt agaaccagac cacagggtca ggttagacgg
     1861 tgccaggtca gcagagccgc cgaggaattc aggcaacagc gggccgaacg cttcgatagc
     1921 attctgcgac gctttacggc tggcgatttt cgccggatta gcctgcagtt tagcgataaa
     1981 ctctttcgct ttggcgtcga agtcagacgg catttcgcct ttcatacggc gggtaaattc
     2041 agccgcttcc tgcggataag ctttcgcgta agccgcaaac ttctcattcc atgcagattc
     2101 tttcgcctgg cctgcttctt tcgcatccca ctgagcatag atttcagacg ggatttcgaa
     2161 cggtgcatat ttccagccca gctgttcgcg ggtcagggca atttcagcgt cgcccagcgg
     2221 cgcaccgtgg gagtcgtgag taccggcttt gttcggggaa ccgaaaccga tgatggtttt
     2281 gcacatcagc agggacggtt tgtcagtcac tgcgcgcgct tcttcaactg cgcgtttgat
     2341 ggatgccgcg tcatgaccgt cgatgtcgcg aataacgtgc cagccgtaag cttcgaaacg
     2401 cattgctgtg tcgtcggtga accagccttc aacgtgacca tcgatagaaa taccgttgtc
     2461 atcgtagaac gcaatcagtt tacccagctt cagcgtaccc gccagagagc aaacttcgtg
     2521 ggagatgcct tccatcatgc agccgtcgcc catgaaggcg taggtgtagt ggtcgacaat
     2581 gtcgtggccc ggacggttaa actgcgccgc cagcgttttt tctgcaatcg ccataccgac
     2641 tgcgttggca ataccctgac ccagcggacc ggtggtggtt tccacaccag cggtataacc
     2701 tacttccggg tggcccggag ttttagagtg cagctgacgg aagtttttca gttcttccat
     2761 cggcagatcg taaccggtga ggtgcagcag gctgtagatc agcatggagc cgtggccgtt
     2821 ggacagcacg aagcggtcac ggtcagccca ggacggattc tgcgggttgt gtttcaggaa
     2881 atcacgccac aggacttcgg caatgtcagc catacccata ggggcacccg ggtgaccgga
     2941 tttggctttc tgtactgcgt ccatgctcag cgcacgaata gcattggcaa gctctttacg
     3001 tgaggacatt ttgactccag atcggatgat gaagggcacg cccttaacga cttgacgaca
     3061 gcgcgttttg ggctacgccg gaaaatttgc caacaattta ccgcaagccg cgcgtcatgt
     3121 acatggaaca tccttttgcc gcttcagaaa tctctggatc atgctcgcat gttgcgcaat
     3181 ctgctcgccc gtccgctgcg cttttcctta tactgagact gagcgtcgat tcacctgcaa
     3241 acggcgcatt tttagaataa tcctgacctt gtgcggaaga gaaaacatga aaattcgcgc
     3301 cttattggta gcaatgagcg tggcaacggt actgaccggt tgccagaata tggactccaa
     3361 cggactgctc tcatcaggag cggaagcttt tcaggcttac agtttgagtg atgcgcaggt
     3421 gaaaaccctg agcgatcagg catgtcagga gatggacagc aaggcgacga ttgcgccagc
     3481 caatagcgaa tacgctaaac gtctgacaac tattgccaat gcgctaggca acaatatcaa
     3541 cggtcagccg gtgaattaca aagtgtatat ggcgaaggat gtgaacgcct ttgcaatggc
     3601 taacggctgt atccgcgtct atagcgggct gatggatatg atgacagata acgaagtcga
     3661 agcggtgatc ggtcacgaga tggggcacgt ggcgttaggc catgtgaaga aagggatgca
     3721 ggtggcgcct ggtacaaatg ccgtgcgagt agctgcggcc tctgcgggcg ggattgtcgg
     3781 cagtttatct caatcacaac ttggtgatct gggcgagaaa ttagtcaatt cgcaattctc
     3841 ccagcgccag gaagcagaag ccgatgatta ttcttacgat cttctgcgcc aacgcggcat
     3901 cagcccggca ggtcttgcca ccagctttga aaaactggcg aagctggaag aaggccgtca
     3961 aagctcaatg tttgacgacc atcctgcatc cgccgaacgt gcccagcata ttcgcgatcg
     4021 catcagcgcg gatgggatta agtaaagcct ggtggtgtcg aacgtaggtc agataaggcg
     4081 ttcacgccgc atctgacatg aatggcacat ttgttacctt gtacgcattg ccggatgcga
     4141 tgctggcgca tcttatccgg cctacgggtg ccgaacgtag gtcggataag gcgttcacgc
     4201 cgcatccgac attaatggca cgttttaccc gtgcgcatcg catctggtgc ttactcgccc
     4261 tttttcgccg cctgaatata cagcatctcc agcgccagcg tcgccgcagc cagagcggtg
     4321 atttccgact gatcgtatgc cggagccact tccactacgt ccatcccaac gatgttgaga
     4381 tctttcaggc cgcgtaccag tttaatggcg cgatcggagg tcaggccgcc aatcactggc
     4441 gtaccggttc ccggcgcgaa agcaggatcc aggcagtcga tatcaaaagt caggtaaacc
     4501 ggcatatcac ccacaatctg tttcacctgg gcgataacgt catccacgct gcgatcgttc
     4561 acctggcagg cgtccagcac ggtaaagccg ttgtctttat caaactcggt acgaatacca
     4621 atctgcacgg aatgattcgg gtcgatcaga ccttctttcg gcgcggtata gaacatagtg
     4681 ccgtggtcaa attcacaacc gttcgcatag gtatcggtgt gggcgtcaaa gtgtaccagc
     4741 gccattttgc cgaaatgctt cgcatgagca cgcagcagcg gcagcgtaac aaagtggtca
     4801 ccaccgaagg agagcatacg cttaccggca gccagcagct tctcggcgtg cgcctgcagc
     4861 ttttcgctca tctcacgggc atcgccaaag gcatatacca gatcgccgca gtccacgacg
     4921 ttcagacgct cacgcatgtc gaaattccac gggaagcggt tgtgttccca ggccagattc
     4981 gtcgaaacct gacggatcgc tgccggaccg tggcgaccac ccgcacgacc agaagtggcc
     5041 atatcgaacg gcacgccagt aatcacccag tctgcatcgc tgtcatacgg ctggaagttc
     5101 atcggcaggc gtaaaaaacc aaaggcattg gaaaccagtg agttatcgta ttgatgacct
     5161 aaggtgctca tggatggact cctattacaa agtcgatata aaaaaaccct tccgcgtcgt
     5221 taggcccgac gaggaagggt tggatttgcg aaacacaagt ttgtgctaat tatcgcctta
     5281 aatgggcgag gcatcaagcg aggttgctat ttccgacacg gtggcggcgc atagccattg
     5341 ttatctgccg gaaaggcttg tccagagcac ggcggcagcg cgttttgcgc cgggccttgt
     5401 aattcaaagg cggcaaagcc gattaccccg gtattacgca catcgccttg cgccgaattg
     5461 gctgcgtagg aatcagcaac gttggcgaac tgaaaagccg cttcagtatg tttatctttg
     5521 cggaatcctt taatcgctaa cgaatctccg gcattcacaa tatagccgtt gttattcagc
     5581 gaaccttgct tgccgttcag cacatccagg ccatcaaccg tagcaacgat ctcgtagttg
     5641 gtgttacgac tgtaattacg gacatataat tgataattca ttccga
//
LOCUS       DADLSB010000212         5611 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.212, whole genome shotgun sequence.
ACCESSION   DADLSB010000212 DADLSB010000000
VERSION     DADLSB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.212"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JN331_003811"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JN331_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY7261808.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JN331_003812"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JN331_003812"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY7261809.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JN331_003813"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JN331_003813"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY7261810.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JN331_003814"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JN331_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY7261811.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1392 a         1351 c         1524 g         1344 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatattttgc ctgaaaagtg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcagcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta acgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg g
//
LOCUS       DADLSB010000213         5586 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.213, whole genome shotgun sequence.
ACCESSION   DADLSB010000213 DADLSB010000000
VERSION     DADLSB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.213"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..200)
                     /locus_tag="JN331_003815"
     CDS             complement(<1..200)
                     /locus_tag="JN331_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HAY7261812.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLS"
     gene            complement(487..1212)
                     /gene="phoU"
                     /locus_tag="JN331_003816"
     CDS             complement(487..1212)
                     /gene="phoU"
                     /locus_tag="JN331_003816"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY7261813.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(1227..2000)
                     /gene="pstB"
                     /locus_tag="JN331_003817"
     CDS             complement(1227..2000)
                     /gene="pstB"
                     /locus_tag="JN331_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY7261814.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2092..2982)
                     /gene="pstA"
                     /locus_tag="JN331_003818"
     CDS             complement(2092..2982)
                     /gene="pstA"
                     /locus_tag="JN331_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY7261815.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2982..3941)
                     /gene="pstC"
                     /locus_tag="JN331_003819"
     CDS             complement(2982..3941)
                     /gene="pstC"
                     /locus_tag="JN331_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY7261816.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4028..5068)
                     /gene="pstS"
                     /locus_tag="JN331_003820"
     CDS             complement(4028..5068)
                     /gene="pstS"
                     /locus_tag="JN331_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY7261817.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5316..>5586)
                     /locus_tag="JN331_003821"
     CDS             complement(5316..>5586)
                     /locus_tag="JN331_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7261818.1"
                     /translation="TSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTD
                     MGKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         1384 a         1475 c         1353 g         1374 t
ORIGIN      
        1 tgacttaaga gttcgcttaa tcgcccgttc agttcatgac tgctcaaggc atactctttt
       61 tctattccac ttgagttaat tctttcgcca gcgcgttttt gaaagccaat tccgcgcccc
      121 atgacgactt tttcccgctg ttgatcatca ataaccacca caacattatt gttgagaatt
      181 ttggtgattt gcatgttcat agcaaggacc ttttttataa acaaaaaaaa cccgacttca
      241 ccagtattct ctggttatgt caggttttgc ctgcgaatgc agtaacaatc cagtcattta
      301 ttaatggttt ttataacgaa catccaggtt cggaaattaa tttaattgcg tgcagagaaa
      361 atagcaatgc gctattgata aaaatatgac catgctcgca gttattaact ttgtgtaatt
      421 ttaggaattt ataaagttat atataacaaa tcccaataat taagttattg ggatttgtct
      481 ggtgaattat ttgtcgctat cttttcccgc cagcagttta tccagctcat caccgcctac
      541 atgacggaaa tcctgaccct tcacgtagta gaagataaac tcacaaatat tctggcagcg
      601 gtcgccgata cgttcgattg aacgcgcaca gaacagcgcg gtaagtacgc tcggaatagt
      661 gcgcgaatct tccatcatgt aggtcatcag ttgacgaaca ataccttcgt attcctgatc
      721 gactttttta tcttcacgat aaatacgtac cgcttcgtca atgtccatcc gcgcgaacgc
      781 gtccagcacg tcgtgcagca tctggatggt atgacggccc agcgactcca gacttaccag
      841 caacggctga tgctgctggg agaatttctc cagcgcagta cggcagattt tgtccgccac
      901 gtcgccaata cgctccagct cggcaatggt tttactgatc accataacca gtcgcaggtc
      961 gctcgccgtc ggctgacgtt tggcgataat gcgcacgcag gcttcatcga tcgccacttc
     1021 catcatgttg acgttcttgt cgccttcgat cacgcgcttc gccagatcgc tgtcctggtt
     1081 atgcatcgcg gtgattgcat cagaaagctg ctgctccacc atgccgccca tggtcatcac
     1141 ctgcgtgcgg atactttcca gttcggcgtt gaactggccg gaaatatgtt tattaagatt
     1201 gagactgtcc ataacgcact cctgaatcaa ccgtaacgac cggtgatgta gtcttctgtt
     1261 tgtttcttcg ctggcttggt gaacagatcg tccgtgttgc tgaactcaat caattcgccc
     1321 aagtacataa atgccgtgtg gtcggaacaa cgcgcagcct gctgcatgtt gtgggtgacg
     1381 atcaccacgg tgtaatcctg cttcagttcg gtgatcagct cttcaatacg cccggtagag
     1441 atggggtcga gcgccgaaca cggttcgtcg agcagcagca cttccgggcg aatggcgata
     1501 ccacgcgcaa tacacagacg ctgttgctga ccaccagaga gagagtaacc gctctggtgc
     1561 aatttatctt tggtttcgtt ccacaatgcg gctttggtca atgcccactg cacgcgctcg
     1621 tccatgtcgg cacgggagag cttctcaaac agacgaacgc caaaggcgat gttgtcgtag
     1681 atggacatcg ggaacggcgt cggtttctgg aacaccatgc ccactttcgc acgcagcagg
     1741 gcgatatcct gagagttggt caggatgttg tcgccatcaa gcagaatttc accttccgca
     1801 cgctgctccg ggtacagttc aaacattttg ttgaaggtac gcagcagcgt cgatttaccg
     1861 cagccggacg gcccgataaa cgccgtaacc tggtttttgg cgatatccag gttgatgttt
     1921 ttcagggcat ggaatttgcc gtagtagaag ttcaaattac gaacctgaat tttactcggg
     1981 gcagtttcaa ccatactcat tgcaatcttt tcctcatcgt acctgatgct tttcgctaat
     2041 caggcttacc gtagggccgg gtaaggcgtt cacgccgcat ccggcaaagt gttaaccgtg
     2101 tttattcttc gcaaaaacaa cgcgcgccag aatgttcagc agcaggacgc acagggtaat
     2161 gatcaatacc ccggcccagg ccagttgttg ccattccgca aacgggctca tcgcaaactt
     2221 aaagatcgtc accggcaggt tggcgatcgg ctgcatcatg tccgtgctcc agaactggtt
     2281 ggagagcgcg gtaaacagca gcggcgcggt ttcaccggca atacgggcaa tcgccagcag
     2341 gataccggtc ataatcccgg acaccgacgc tttcagcgta atcgcggaga tcatcttcca
     2401 cttcggtgta cccagcgcat aagccgcttc acgcaggctg tccggcacca gtttcagcat
     2461 gttctcggtg gtgcggataa cgatcggcac ctgcaacagc gccagcgcaa ttacgcccgc
     2521 ccagccggag aagtgctcca tctgtgccac cacgatggtg tagacaaaca gaccaaccac
     2581 aatcgacggc gcggagagca gaatgtcgtt aatgaagcga atcacttctg ccagccagga
     2641 tttacgacca tattccgcca gataaatccc cgccataatg cccagcggcg taccgaatac
     2701 cgtggcccac aaaattaaca gcccgctacc cgccagggcg ttcgccagac caccaccttc
     2761 cgtattaggc ggcggcgtca tttcagtgaa cagcgccagc gacataccgt cgataccgcg
     2821 agtgatggtg gacattaaaa tccagatcag ccagaacagg ccgaaggcca tcgtcgccat
     2881 cgagagcgtc agcgcaatac ggtttttgag gcggcgacgc gcctgcattt tgcggcgaga
     2941 ttcagccagc gccgcagtgg tttgcatttc aaccatagcc attagcgtgc cccctcattc
     3001 ttagccaggc gcataatcat aaacttcgat gcggcgagga cgataaaggt aatcacaaac
     3061 aggatcaggc ccagttccat cagtgcggca acgtgcagac cggattccgc ttccgcaaat
     3121 tcgttcgcca gcgcagaggt gatactattg cccggcatgt acagcgaggc gctgtcgagc
     3181 tggtaggtgt taccgatgat aaaggtcacc gccatggttt caccgagcgc gcggcccagc
     3241 cccagcatga tgccgccgat aacaccattt ttggtgaacg gaagaacgat acgccagata
     3301 acttcccagg tggtgcagcc aataccgtag gccgactctt tcatcatcac cggggtttgt
     3361 tcgaacacat cacgcattac cgccgcaatg tacggaataa tcatgatggc gaggatcacg
     3421 cctgccgcga ggataccgat accaaatgcg gggccagaga acagcgcgcc aacaatcggg
     3481 atattcgaca tgatattgcc gaccggctcc tgaaagtaaa cggcgaacag cggcgcaaag
     3541 ataaacaggc cccacatgcc gtaaacgata cttggaatgg ctgccagcag ctcaatggcg
     3601 atacccagcg ggcgtttcag ccagccaggc gcaagctcag tcaggaacag ggcgataccg
     3661 aaactcaccg ggacggcgat cagcagcgcg ataaacgaag tcaccaacgt accgtagatc
     3721 ggcaccagcg ccccgtagat atcgttcggt gcatcccact ctttggtcca taggaaagcc
     3781 agaccaaatt tctgaatgct cggccaggag gagatgatca gagagacaat aatgccaccc
     3841 aacatcaata gcacaatcag cgccgccagt tttaccagca cgctgaaaat tatgtcgccc
     3901 tttttacccg gtgggttaaa agcgggcttg gttgcagcca taagttactc ttcagttaaa
     3961 cgcgtttaca aagtcatttt gtaatgccgg atgcggcgta aaacaccgta cccggcctgg
     4021 agttttatta gtacagcggc ttaccgctac tgtctttaat attggtcttc cacgcagcgc
     4081 gaacctgttc aactacacta tccggcaggc tggcgtaatc caggtcgttc gcctgtttag
     4141 ccccggtttt gtacgcccag tcgaagaatt tcagcacctc tgtgccttgt tctggtttct
     4201 tctgatcttt gtggatcaga atgaacgtgg tagaggtaat aggccatgca tcttcgcctt
     4261 tctggttggt cagatcctga gcgaaggttt tgctccagtc tgcacctttt gctgcattag
     4321 cgaagttttc ttcggtcgga ctaaccggtt taccatcagc ggagatcagt ttggtgtacg
     4381 ccaggttgtt ctgcttcgcg taagcatatt caacataacc aattgcaccc ggcagacgct
     4441 gaacgaacgc ggcgataccg tcgttacctt taccgcccag accgatcggc cattttacgg
     4501 tagagccagt accaacgttg tttttccact cttcgttcac tttcgccagg tagctggtga
     4561 agacgaagga agtcccggag ccatctgcgc ggcgtactac agcaatgttt tgtgaaggca
     4621 gtttcagacc cggattcagt ttggcgatgg cttcatcatc ccacttcttg attttgccca
     4681 ggtagatgtc gccgagggtt ttaccatcca gcaccagttc gccagacttc agccctggaa
     4741 tgttaaccgc cagcaccacg ccgccaatca cggtcgggaa ctggaacaga ccttcctgag
     4801 ccagtttttc gtcagacagc ggcgcgtcag aggcaccaaa atcaacggta ttagcgataa
     4861 tctgttttac gccaccggaa gaaccgatac cctggtagtt aactttatta ccggtttctt
     4921 tctggtaagt gtcagcccat ttggcataca ccggcgcagg gaaggttgca cctgcacctg
     4981 tcaggcttgc ttctgcaaac acagagaaag cactcatcga taaggtcgcg gcgacaacag
     5041 ttgcgacggt ggtacgcata actttcataa tgtctcctgg gaggattcat aaagcattgt
     5101 ttgttggcta cgagaagcaa aataggacaa acaggtgaca gttatatata aggaatatga
     5161 catttttatg acagaaagga attaccaaat aacttactga ttttaaatgt gaagatgaaa
     5221 atcccacatg ccatcttttt gtcatgatgc atttaaaagt gataaagaaa tgggtgagat
     5281 gacaacgatg tgataaacaa tcgttgtcag acctattagc gcacaatctc aacagttatc
     5341 gtcgctgttg cagtaaattg ccccggggct gggcgagctc cagtaaggct cgcaggagca
     5401 gctgaaaaag ttacactacc tgatttttca ccatacactg ttgatttacc catgtctgtt
     5461 ggcaactcac caccgttcac tttgatttcc ttattatttt tgtcgtaaat ttttatcccc
     5521 acgttagagt ttgaagttac gaccattgaa ttgtccgtat tgggttcggt agcataaaaa
     5581 gacgta
//
LOCUS       DADLSB010000214         5581 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.214, whole genome shotgun sequence.
ACCESSION   DADLSB010000214 DADLSB010000000
VERSION     DADLSB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.214"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..502)
                     /locus_tag="JN331_003822"
     CDS             complement(<1..502)
                     /locus_tag="JN331_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261819.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSK"
     gene            complement(510..2858)
                     /locus_tag="JN331_003823"
     CDS             complement(510..2858)
                     /locus_tag="JN331_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7261820.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEVIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(3036..3947)
                     /gene="gsiD"
                     /locus_tag="JN331_003824"
     CDS             complement(3036..3947)
                     /gene="gsiD"
                     /locus_tag="JN331_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY7261821.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(3950..4870)
                     /gene="gsiC"
                     /locus_tag="JN331_003825"
     CDS             complement(3950..4870)
                     /gene="gsiC"
                     /locus_tag="JN331_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY7261822.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(4888..>5581)
                     /locus_tag="JN331_003826"
     CDS             complement(4888..>5581)
                     /locus_tag="JN331_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="with GsiACD is involved in the transport of
                     glutathione into the cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY7261823.1"
                     /translation="PKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKP
                     WPYDPVKARELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMD
                     AGQRAAEVEGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNK
                     QVDDFLAQALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMP
                     DTGFSFEDADLQ"
BASE COUNT         1582 a         1443 c         1183 g         1373 t
ORIGIN      
        1 attttgacag taaagaaaaa cgctccagac caagcaaaaa tatcagacta cgattgtgag
       61 ttaccggata gaggtaatag acgaaggttt taccgtcaaa agagataaac cctgtatttt
      121 tgacatgtcg atacttcaca acctgcataa tatctgcaca ataatttttc tcgaactcct
      181 ttacctgaat acattcgtca ctggaaatca gataaggacc gacatcgtac aaataagagg
      241 gggaaaccga gtcggcgtaa gctgtgcgca aaccaaccaa cagctgttcc tccgtgggat
      301 ttagcgtgtt gataagatca attgccacgc tacgccgata ttcagcaata tttgccaccg
      361 caaaagcagt atcaataacg gactgtttca ggctatacac ccgttccttt accatttgag
      421 tgtagatccc gcaggcaact gctgaaatca tgataaaaat cagcagactg actgtaagta
      481 cgaacttatt gattctggac atacgtaaac tactctttta ctaattttcc acttttatcc
      541 caggcggaga atggcatggg tttctggtac agatacccct gagcctgaac acatcctaat
      601 ttaattaatt cgcgcgcaac gtcttcgctc tctacgcctt ccgcaatcac cgggatccgg
      661 ttatagcgtg ccagatcgaa aataatgcgc acaatttttc ccgcagtacc gaaaatatcg
      721 ccggtaaatt gcgagtccac ctttatgctg tctggattaa tttcataaaa acgtttcagg
      781 tttgacaaac cggtgccaaa gtcatcaatc gatatccgta ccccttgttt acgcagttgt
      841 tcaatattga gataaaaatg ttcattaatt cgctcgatca cggtttcagt gatctcaaca
      901 cagaggctgt tcgccttcag gccattttct ttcatgatgg tggtaaaacg ctcatgaaag
      961 tgcggttcgt ttaactgcca gggcgaaaca ttaatatgca gtaagaaatc atccttcagc
     1021 gacgcacgtt gactaaaatg tctgaattca cccattgccg ttttaataat ctgataaccc
     1081 agctcattga taaacccgat atcttcagca atggtaatga acttcagcgg tgaaatgatc
     1141 ccccgctccg cagaaaccca acggcacaac gcctccgcgc tcagaatggc gcgggtgtta
     1201 atatcaacga tcggttgata gacgaggaat agttcgccaa tttccattgc atggcgaata
     1261 ttacgcgcaa tccagatatt ctcttctatt tcgttgagtt tcgcgtcatc acagaaaata
     1321 aactcggaga acatatggtc acggatactg gaaatcgcca gtttcagttt ttctgcgtaa
     1381 ctccaggcgt tttccccctg caaaggttca caaatggcag acttatggat gcgcagattg
     1441 cggtagccaa agctgctgtc aaaatcgttt accatcgcta ttttacgggt aaatgtctgt
     1501 acattttccc gacacagaag taaatagaga tcggcattga atgtcacgat atcgcagcat
     1561 tgcgcatatt tctcggcaat tcgctgacac aatttcctga cgattttatt gcgttcatca
     1621 acaccaaaca ccgcttccac ggcttccaga ccacacagtt tgatgatccc cacagccaga
     1681 tactgattat tgttggactc aataatcgcc atatttttaa taaaaccgct ttcctgatcg
     1741 taagagatca gtgagagcaa tttatttgac cgttcaattt cggcatcttt catttccgcg
     1801 atcgaatttt tgatgctgat aatctctttc gcatcgcctt cgcgaatttt cagagcctcg
     1861 atctgatccg ttgagtcggg caaaaacgcc agcgtattaa ccagctcggt catttcagaa
     1921 aattgccgac ggaacacacg agtggtcatg aaatatacca ccagcaacac gataataatg
     1981 aacgcaaacg gtagataaaa aagtttatct gatgcgttca ataaattacc gccaggataa
     2041 taaaaataga tttcgaacaa tgcattattt ggcactgctg atgtctttgc aatatactcc
     2101 gtatcgacag aatcaataat attataatta ccttcgctat tataaataat cagtttttca
     2161 cgagcgagtt gattcttggc aatgacctca tgaccttgtt tcaaaaatag aacttcatca
     2221 ttagcgctga tttgatcaag atactcgttc agataccgcc agtttaaatc gacatgaatt
     2281 ttatctaccg ctaacaaatc gtgatctttg ccgttgatac gtagcgtaaa agagatacat
     2341 tttttacccg gacgatcaaa gcaattgtat gccggattcc agctgttgtt agagtcatct
     2401 gaagccaccg accacggtcg tttcgatggg ttaaaatcgg caaaaataac ccgccctgac
     2461 tcgtcgacaa gcggtccatt tacttgttcc tgatgataaa caacgatttt atcattcgcc
     2521 ctccgcgaaa agagataata tttattatca tcaagtacca gtcccaccga gtctaaaaat
     2581 ggcatcattt ccattttcag aatcacggcg cgttgaatct cctctgtatt ttttaataaa
     2641 tacagtgagt cctttaatgt cagggccgaa cgttcaatat ctttaaagaa atagccaacg
     2701 tttttctgaa taagttcaaa gcggctatcg gcacgtttta ccgcgcgttc cgataccagt
     2761 tgatagttaa ttatgaaaaa aagaccaata aatgacagtg ctgccaataa aaataaaatt
     2821 atcagcctta tctttatctt ttcgtataaa ctcagcatgt taatacattt aacaaaataa
     2881 ctatctgaac gctgtgccgc tgcaaaaata gtcccttgct gcggcttttt aaccaaatta
     2941 cccgattcta caaaatcagc agaaagttac aacacttgct tagggcaata atttcctgtg
     3001 aggtgattac cctttcaagc aatattcaaa cgtaactatc ctttaatttt cggatccagc
     3061 gcatcgcgta aaccatcgcc caacaaattg aacgccagta cggtcagaaa aatagccagg
     3121 gccggaaaaa cagcgacatg cggcgcgata accatatccg ctcgtgcctc attgagcatt
     3181 gctccccact ctggtgtcgg cggctgcgca ccgaggccga gaaatgacag gctggcggca
     3241 gagataatcg aggtgccaat gcgcatggtg aaaaacacca cgatagaaga gacggtccca
     3301 ggcaggatat gacgcaataa aatggtcata tcgctggcac caatactgcg tgctgactca
     3361 ataaaggttt gctgtttcaa caccagcgtg ttgccgcgca ccaggcgggc aaacgcgggg
     3421 atggaaaaaa tggcgactgc gataatcacg ttagcaatgc cgcttcccaa caccgcaaca
     3481 acagcgatcg ccagtaaaat acccgggaag gcaaacagca catcgcaaat gcgcatgatc
     3541 agccgatccc accagccttc ataatatcca gccaacaagc ccagcaacgt ccctatcgcc
     3601 acaccgataa acacggcaaa cacgcccgcc gccagcgaga tttgcgcacc aaccaggaca
     3661 cggctgaaaa tgtcgcgccc cagtgaatcg acgccaaacc agtgctgcaa agaaggtccg
     3721 ttattcagat tgtcataatc aaaataattt tcggcgtcat agggagcgat ccagcgtgca
     3781 aaaatggcca ccacaatcaa taaaataacg aataatgcgg cggtcatcgc catatgctga
     3841 cggcgaaatc gtcgccagaa ttcatgccac ggtgtacgta cctggtcagg tttgaccagt
     3901 ggcatggcgt ttaacaccgc ctgacgtcgc cagttaaata gtcgcatcct tacttgtacc
     3961 tgatagccgg gttaatggcg gcgtaaagca catccaccac taagttgata agaataaatt
     4021 ccagcgagaa aagcagaatt tccgcctgaa tcaccgggta atcgcgcatt tctacagagt
     4081 caacgagtaa gcgtccaagt ccgggccagt tgaaaacttt ctccaccacg atagaaccac
     4141 cgagcaaaaa gccaaactgt aagcccatca tggtcactac cgggatcatc gcgttgcgta
     4201 gcccgtgttt gaggacaacc caggtttcgc tcaccccttt cgccctcgcg gtacgcatat
     4261 aatcttcgct taaaacgtcg acaaatgacg cacgggtaaa gcgcgccatc acggcggcca
     4321 ctgccgcgcc gagcgtcagg gagggtaaaa tgtagtgctg ccaactgtct gctcccacag
     4381 taggcagcca gcccagttca acggagaata cctgaattaa aagcatcccc agcgcaaatg
     4441 ccggaaacga gatccccgac accgcaatgg tcatactcaa tcgatccggc caacgattac
     4501 gccagacggc ggcgataatt cccgccgcca taccaaatat caccgcccag accatactgg
     4561 ttatggtcag ccacagcgtt ggcataaagc ggctggcaat ctcatcggca accggacgac
     4621 gcgacaccat cgacaggcca aaatccccct gcacagcatt gctgatatag tgccagaact
     4681 ggtgatacag cggctgatcc aaccccagct gctgacgcac cagttctata acctgcgcat
     4741 cagcttcggg cccggcaatc aatcgcgccg gatcgccggg cagcatgtgg acaaataaaa
     4801 acaccagcac cgagacgata aacagcgtcg gaatcaaccc cagtaatcgt ttgataacgt
     4861 aattaagcat tccactccct gcgttgctta ttgcaaatcc gcgtcttcaa agctgaagcc
     4921 ggtgtctggc atgatccaaa aaccggtcag gtttttactg tgtgccgaca ccagtttttc
     4981 taccaccagc gggatccacg gcgattcttg ccagatgata tcctgcgccg ccttatataa
     5041 gcgggtcttt tccgccggat cattcgtttt cagtgcctga gccaggaagt catccacctg
     5101 tttattgctg taaaacgcgg tattaaacag cgtcggtggc cagttttgcg aggcaaacag
     5161 cggcgatagt gcccagtcgg cttcgccggt tgaagccgac cagccagtgt agaacatccg
     5221 cacgccgctc tctttttgcc ctttaccttc aacttctgcc gcccgctgtc cggcatccat
     5281 cgcggtcacc tgggctttaa tcccgacctg cgctaactgc tgctgggtaa attgcagcac
     5341 tttctgcgcg gtgctgtggt tatgtgacga ccacagcgtg gtactgaaac cgttgggata
     5401 tcccgcctct ttcagtaatt cgcgcgcttt cactggatcg taaggccacg gtttataact
     5461 ttgcgcgtag gcgatacttg gcggtaccac accagtagct ggcgttgcat agcccgcaaa
     5521 ggcaactttc accagcgccg gacggttaat ggcgtaattc agcgcctcac ggaccttcgg
     5581 g
//
LOCUS       DADLSB010000215         5580 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.215, whole genome shotgun sequence.
ACCESSION   DADLSB010000215 DADLSB010000000
VERSION     DADLSB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.215"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..1160
                     /gene="espX1"
                     /locus_tag="JN331_003827"
                     /pseudo
     CDS             1..1160
                     /gene="espX1"
                     /locus_tag="JN331_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            1482..2015
                     /locus_tag="JN331_003828"
     CDS             1482..2015
                     /locus_tag="JN331_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7261824.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSEMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            2065..2748
                     /locus_tag="JN331_003829"
     CDS             2065..2748
                     /locus_tag="JN331_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7261825.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAEKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSNNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            2761..5212
                     /locus_tag="JN331_003830"
                     /pseudo
     CDS             2761..5212
                     /locus_tag="JN331_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            5225..>5580
                     /locus_tag="JN331_003831"
     CDS             5225..>5580
                     /locus_tag="JN331_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY7261826.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLLETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDDKKIEL
                     PGKSSYNLKEMLSAIN"
BASE COUNT         1924 a         1065 c         1013 g         1578 t
ORIGIN      
        1 tcatagaaca tacagttcaa ttttcttttt ttaaacacaa aggcatcaca ttacttagat
       61 tggatgtttt tgatgacaga acaagcgaat gtctttctga agagattaag atatatcaag
      121 aatgccatga aaaatttatt aaatttttaa aggcaaactt caaccaggaa atatacccag
      181 agctatacac tcctgaaata ttttatgagg cctgcagaaa tctccagtca ttttatgatc
      241 atcaggagtc gtcacaaaac gctaaataca gtgcaatagt caagaaaaaa agttatttta
      301 ataaggaaat tagaaatcta ataaaaaaga atatttatcc tgagctatac aatgagcaat
      361 gcaataaaat ccccgcctca tcgacagatg ataaccagaa aataacatgg cagaatttta
      421 aaactagcaa tgcggcatat tcacaactat gtgaaaaatt atctttatta aaaagctcgc
      481 ccagtcggtt aatagagaaa tcagcttact gctcaaatga aaacatgatc accgataaat
      541 ttaatgttgt cttttcttac tgcggagata atgtaaatga atttatactt ttattaccct
      601 ataataaatc tctggagatg catgaactta acgagcaaaa catccagtat ttgacttccc
      661 ttaatattaa tatacataaa atgttgctat caaatataac aatagaaaag agcaaccttt
      721 catatgggta ttattttggt tgtgtattat ccaatatatc atgttttgaa agcgacttat
      781 caaacaccat cttttcaaat ggagaaataa acaatccctt cattaaaaaa agcaacatat
      841 ttggaacatc gttcactaat accatgataa aaaatttgcg ctgtgaagat attatgcctg
      901 gccgatggac cactcaactt gtcaataagc acttaggtta cagatacaca ggagtattca
      961 aaaccctggc aagtattgat gacaaaccgt cccgtttcga aattttgata ccattggtac
     1021 aaactttagt tcgcgacaat gtaaagctaa ataatgatgt gtataaagaa ctgaaaaagt
     1081 ttatgcacga ttacgacaaa acatccccag aaatgagaaa ataccttcag agcatcaacg
     1141 agtctatgtt ccttataaaa aaatatctca tcaagattaa aacaccccaa aaaataagta
     1201 atttcagaat gaaactacct gtgataatta ttaattaact gcagagaaat aaaaaataat
     1261 ctgaagcatg ctctgaaaca tcctactctg tcactctaat gatggcacct tgctcttctt
     1321 ataaagaagg gaataattaa gaatattaaa ttttcaaact ttcatcaact tcacatcaag
     1381 aaattaaaaa atttaaacat taatctaaat aataacaacc tgatcacata atcgggtttg
     1441 aaaacgagtg accttccata ccagttaatt aaggatgatt tatgaaaaag tatattattc
     1501 ccttcgttgc gacaacgttg atgtttagcg caacaacctt cgccaaggat gtcgatcaag
     1561 gaacactaac cattagagga gaagttgttg atacaacgtg ttactttgtt aatggtgatt
     1621 cgacaacaga tatcgttgtc gaagatgttg taaccagtga aatgaatcaa cttatcaata
     1681 atgaaatcta tactttaaaa accggttcaa cctctcatga cttagaaatt cagtgtgacg
     1741 gaaatacagc cccaagtatc gaagtactag catcagagtt tgatgctaat aactttacgc
     1801 tgaataaagg aaccgctgag aacattggct ttgccatttt cctcgatgat gggactttga
     1861 ccagaatgag accagggtta aaaacaccgc tgaaagataa tggctctggt caatattttc
     1921 ttaatttgaa tgcacaatat gcccgcacaa gtggcaatcc agttacaaaa ggctccgtag
     1981 agtcaaccgt aacgattaaa atcagtgcag attaacgttt tttgccggta gctctgctac
     2041 cggtattatt tacggagtga ttacgtgaga ataataacag cattactgat gtcatttttt
     2101 attttacctg ttcatgccgg tgtggtcatc tatggtacgc gcattattta tcctgaaaaa
     2161 aacagagaag ttctcgtaca gttaatgaat caaagtaaaa atgcctcttt aattcaggca
     2221 tggatcgatg atgggaacac cactatcgcg ccagaaaaaa ttcaggtccc atttattctc
     2281 acaccgccag taagtcgtgt agcaggtggt tctggccaac aacttaaaat aagaaaaatg
     2341 ccaaacaact tgcctcacaa taaagaaagt ttattttatc taaatgttct ggatatccct
     2401 ccaaacaacc cacaaaatgc agaaaaaaac aaaattaaac ttgcattgca aaaccgtata
     2461 aagctacttt ggcgtccaag cggaatagct ccggtagata aaaaaagcct ttctcaatta
     2521 aatatcaaaa agaaaaataa cgccatctca ataaacaacg aaacagccaa ctggataacg
     2581 gttacaacta tcaaagcaca aaacgtgaaa gttaataatg aaagtatctt actgcctcca
     2641 ttttcaaata acgacattac attaaaaaat aaccacgcat cagaatatga actaacggta
     2701 gttgatgatt acgggaataa catccatagt aaaatcgccg cgagataaca ggaaaaagtc
     2761 atgttaagga cgacacgatg ggtcgctgct attatttttt tgtattcttt tccgggttat
     2821 gctgaagaaa attttgatac gcactttatg agtggcggaa tgagaggaga aaaagtttcc
     2881 gaatatcgtt tcgataataa acaaccgctt ccaggaaact acgaacttga tttttatgta
     2941 aataaccagt ggcgtggaaa acaagatatc acgatcccag agtcaccagt caaaccatgt
     3001 ctgcctaagg tactactaac caaactgggg gtaaagaccg gtaacctcaa tacagaagat
     3061 aattgtattt tactggatga agcggttcat ggtggacaat atcagtggga tatcagtgaa
     3121 caccgactaa atttaacagt accacaggcg tatatcaatg aacttgaaag aggctacgtt
     3181 cccccagaaa gttgggatcg cggcattgat gctttttata cctcctataa tttaagtcag
     3241 tatcgctctt atgacagcaa taataacagt catacagcca gttatggccg gtttaacagc
     3301 ggattaaatt tatttagttg gcagttacac tctgatgcca gttatagcaa acctgatgat
     3361 atgaaaggca aatggcaaag taatactctt tacctggaac gtggctggtc gcagatatta
     3421 agcacagttc agataggaga aaattacaca tcctcactca tattcgactc tctgcgattt
     3481 agcggaatac gcttatttcg cgatatgcaa atgctacctg attctatgca aagctttaca
     3541 cctttagtac aaggtgttgc gcaaagtaac gccttgataa cggtttcgca aaatggctat
     3601 accatttacc aaaaagaggt tcccccagga ccatttacta tcgcagattt gcaactttcg
     3661 ggcagcggct ctgatctcga tgtcagcatt aaggaagcag atggtagtgt tcgttccttt
     3721 ttggtgccct actcttccgt gcccaatatg ctgcaacctg gtgtttcaaa tttcgatttt
     3781 atcgccggac gtagtcagat atatggtgtc aagaatcagg aggactttct cgaggcaaat
     3841 tatatatatg gcctcaacaa tcttttgaca ctttatggcg gtacgatttt atccgataac
     3901 tataatgcta taactctggg aaatggttgg aatacaccgc tgggagcaat ctcttttgat
     3961 gctacccgat cgagtagtaa gttaaataat gataccagac acgaaggtac gagctatcag
     4021 gtcgcctata ataaatatct attgcaaacg gccactcact ttagcgtggc cgcctagcgt
     4081 tatgcttcgc aggattacag gacattcagc gaccatcttt acgaaaataa taaaataaat
     4141 catcagagtg actatgatga tttttatgat attggcagaa aaaaatagcc tttctgccaa
     4201 tataatgcaa cctttatcca ataatctggg aaatgtatca ttaagtgcac tttggcggaa
     4261 ttactggggg cgaagtggaa atgctaaaga ttaccaattc agttactcca ataactggca
     4321 acacattagt tatactttct ctgccagcca atcctatgat gaaaacgata aagaagagga
     4381 gcgttttaat ctgtttatct ccattccttt ctactggggt gatgatattg ccaaaacacg
     4441 tcaccaaatt aacttatcga attctacctc attttccaaa gatggctatt cctccaacaa
     4501 tactggaatt actggcatag ccggtgaaca tgatcagtta aattatggta tatatgttaa
     4561 tcagcaacaa caaaataatg atacctcgct tggtacgaat ttaagctgga gaactccaat
     4621 cgccacaata gatggcagct atagccattc taaaaacgcc tggcaaagtg gtggaagtat
     4681 tagtagtgga ttagttgtct ggcccggtgg tattaatatc actaaccagc tatccgatac
     4741 atttgcaatt ctggatgcgc ctgggttaga aggcgcgcat attaatggac aaaaatacaa
     4801 ccgaacaaac agcaaaggcc aggttgttta cgacctgatg atacctcatc gtgaaaacca
     4861 tctggtactt gatatagcaa acagtgaaag tgaaacagaa ttgcagggca atcgtcaaat
     4921 tattgcgcct taccgtggag cagtttctta tgtgcagttt acaactgacc aacgtaagcc
     4981 ctggtatata caggcactgc gtcccgatgg ttcgccatta acctttggct atgacgtact
     5041 ggatctccag gaaaacaata ttggagtcgt tggccagggt agtcgccttt ttattcgcgt
     5101 agatgaaatt cccactggca taaaagttgc tctcaatgat gaacagaatt tattctgtac
     5161 tattactttt caacacgtta tcgatgaaaa caaaacatat atatgccagt aaaaggagtt
     5221 tactatgaag tggcttttat taatcacctt gagtctatat tcttttattg tgcaaagtgc
     5281 tccctgtgga ttgaccaatg tcggggaaca acgtggaaca tatatactca agccactgag
     5341 tatgaaaggt aatctcaccg ccgaaaaaat tgaaaacacc ggaaatatca ttgagtgtgc
     5401 agacttgcta gagacgacta acgccattaa agttcatttt ttaacacaga acgctttaag
     5461 gtttaaagtc ggaagtcgtc attatattat taatttcccc tttgaatctg acgataaaaa
     5521 aatagagctt ccagggaagt catcttataa tttaaaagag atgctatctg cgataaattt
//
LOCUS       DADLSB010000216         5549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.216, whole genome shotgun sequence.
ACCESSION   DADLSB010000216 DADLSB010000000
VERSION     DADLSB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.216"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            94..195
                     /locus_tag="JN331_003832"
                     /pseudo
     CDS             94..195
                     /locus_tag="JN331_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(190..606)
                     /locus_tag="JN331_003833"
                     /pseudo
     CDS             complement(190..606)
                     /locus_tag="JN331_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(651..1085)
                     /locus_tag="JN331_003834"
                     /pseudo
     CDS             complement(651..1085)
                     /locus_tag="JN331_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1097..1318)
                     /locus_tag="JN331_003835"
     CDS             complement(1097..1318)
                     /locus_tag="JN331_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261827.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(1319..2002)
                     /locus_tag="JN331_003836"
     CDS             complement(1319..2002)
                     /locus_tag="JN331_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY7261828.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2066..2377)
                     /locus_tag="JN331_003837"
     CDS             complement(2066..2377)
                     /locus_tag="JN331_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261829.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(2374..3345)
                     /locus_tag="JN331_003838"
     CDS             complement(2374..3345)
                     /locus_tag="JN331_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY7261830.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFSGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLKVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            3716..4675
                     /locus_tag="JN331_003839"
     CDS             3716..4675
                     /locus_tag="JN331_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7261831.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAIIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQLALVKAMVHA"
     gene            4675..5070
                     /locus_tag="JN331_003840"
     CDS             4675..5070
                     /locus_tag="JN331_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7261832.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5253..>5549
                     /locus_tag="JN331_003841"
     CDS             5253..>5549
                     /locus_tag="JN331_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY7261833.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRR"
BASE COUNT         1532 a         1443 c         1334 g         1240 t
ORIGIN      
        1 gaccggcagg aaaaaggcgg ggataatgag atggtcaccc ccaccctaac agcagtacgc
       61 tgttaaggtg aatcccttaa cggagagacc atcatacaga acatcacact tattggtatc
      121 gatctcggaa agcattcctt ccacattcat tgtcaagaca agtctggtaa ggctcttctg
      181 cgtaaaaaag atacgccgcg catcttctgc cccccggcaa ctgacactgc atactgcatc
      241 gcgcaccgca tcaagaactg ccctgatttc gtcttcggtc agccattcac cggcatacgg
      301 gaaagacacc tgcgttccgt ccgccggaag taccggcagc gactgccact gactgccgga
      361 cagaacaagt tccgctccgg ttccggtcag tttctgaatg gcggaggcca ggtgcggcgg
      421 cagcacgcca tcgtggcggt taacgagtaa cgtcgccagc cggggcgcat ccagccgcac
      481 gtaaatgctg atccagcccg gatagttcgg gttacgttgg tcaacatccg tctcttccgg
      541 ccagccctgc gccagcagca ggttacgggc gtgacgacaa atcagagcat tcagcgtttc
      601 attcataatt catgaactcc ctgtatcgcc gcccgcaggc ggcgataaca tcagtcgttt
      661 tcgtgtgtgt tgtttttcag caggctgcgg gcctgatcaa ccatcgcctg tcggcgggta
      721 acgtattccg ggagggggaa accgtgatcc cgggcagccc attcacgttt ccacagtgcc
      781 tgttcagcga tctgagtcag ggtctccagc agttcagcag ggacggtcac ctggcggttt
      841 tcgcgcacgt tgctgataat ctccatcgcc gcggcggaag aaataccgga ctcaatccgc
      901 tagtcttccg ggatgtcctc caggtgcgcc aggacggtcc tgatttcatg atcggacagg
      961 gaaatatcca ttccgtcagc gagggcctga atctcttccg gggaccagat aaccagcatc
     1021 agcggcgtgt ctcccgaata ctttgctgcc agcgcccggc atagcgtttc gcatgttccg
     1081 tacatacact ctcccctcaa tctttgaagc aggaatccag ttccagaatt tcggtatatg
     1141 cctccatcaa gaggcggttt gcgcgaaaca acgtctgacg caccagacgt tccggcaggg
     1201 tgtcagcatt aaagctgtac cgccgctcca tctcattcat ggttgtacgc aggtcgcaca
     1261 tggcattggc cacctccgcc acttcggcgc gaagtttttc gtgtccggca tagttcattt
     1321 acgcagcctc cggcataaac cagtcatcat tcgcggcccc ctgctgcatg gcccgctgta
     1381 cggcagccag tcgttgctgt ccggcacggt gatactgctc aagcagctcg ataccgatat
     1441 accggcgtcc ggactggagg gcggcgacgc aggttgaacc gctgcctgca aacgggtcca
     1501 gcactatcgc attcgggtgt gtgaagctct caatcagcgg ttgcaggctg gtaacgggct
     1561 tttccgtcgg gtgatggcgg ttgcccgaat atttccagcc caacacgtcc ggcagcgggt
     1621 tttgtggcag acgtggacgg ccttttgcca ggatgtaggc gcattcgtgg cgatagccca
     1681 catatgcggc cttcgatgtg tagtttttgg tgaagaccag gtgaccaaca acgctgaatc
     1741 ccgcattttt ccaggcagac ataaagcgat cgacgcggtt ccagccatag aagctcacca
     1801 tcagcgcgtc ttttttgagt acgcggtaca tttcattaca ggccggttgc agccattcat
     1861 cagttttatc accagcaatg gtacgcccct gacggtcacg aaaaccgacg agatatggcg
     1921 gatcggtgag gatgaaatca atggcgttat caggaatacg ggccataaca tcgatgcagt
     1981 taccgaggac aaaacgggac ataacagatc cttctcttac agagggcacc cctgtggtgc
     2041 ccgttccccg ctgcctgtgg gctgttcacc gcgtccggta acgggtcagg ccgaccgtgg
     2101 aataccgcag ccgtcgtctt tttgacagca aggttatgcc gcgaaagcgg cttgaagggc
     2161 gttcatcaga aaagggcgac gggctggcgt acactgtcac cgacgccagc cgggagcggt
     2221 aaaagcgccc gtcccgtgcc cgccggacgc gggacactga ccacaggcag cgtttatgcc
     2281 gcgccgcgtc agccggcgca gtaaaaagag gagcacggcg acatcctttg tcggaaaaga
     2341 aacgggcggt tgcccgcccg tctccgtcat acttcatccg ccataatgcg ccacattgtc
     2401 gactatccac gcaggtccgg tgacttccgg ctgctcatcg tcatctgtcg gggaagacca
     2461 ttcaagttcc ccccacagca catcgacgag ctctccacgc tcatacaact gccagccaca
     2521 gaaatcacag ccctgttcgg cataccagtg ctccagcgtg cagctgaaac ggcggcttaa
     2581 ttctgcaata acgtcgcttt ccggctgaca ccacggcgtg tcgaaatcca cctgaatgaa
     2641 gttttctccc tgactgctga tattcaggtc atatccacaa ggccacttca cgccataccg
     2701 ttccgtatac cagtgatatg cagaaggaac accgttcaga acaccaccat taaagccgtt
     2761 cacttcaaca tccaggcggg tcggcagaac cttcagcagg tcaaacggca tggttttttc
     2821 cggcactacg ttgtcacaca gacggttcca ccacaccgat acatcctcgt taccccatat
     2881 gtcacaccag tcacctcttt ttgcggtgaa atgcacagtg atggtttccc tggcatcatc
     2941 cggtaatcct tcccagcgac gctggccagt accgctctgt aaccagagtt catgcagcat
     3001 caggcagttt tgttcatcca gcaacacacc gttctgcagg tgtgtcagcc agcgggtgaa
     3061 cgcgatattt tccgtcgata cagcaccacg tccggcagcc gtcactccgg ggcactgttc
     3121 agagcggata ttttcagtga tctgcagaag tccggcactt ccggccagaa acagctgaat
     3181 accttcattt gtggcgcggc gataaagcgg tgtgactgca ccgctggcca gtcgtttaat
     3241 ctcagcgatc tgtgccggtt caccagaaaa atacatacga ttcgagcacc agtttggcat
     3301 gggaattctc ctctcacatg cggcctccat caggccgtgc cgcacctgta accgggcgac
     3361 cgcatacgaa tgcaggtgcg gccccgaagc cgcagcgcgt ggaggaggaa cgacgaaacg
     3421 tgaccgggtg aggaggccgg cgacagcgac acacttgcat cggatgcagc ccggttaacg
     3481 tgccggcacg gcctggttaa gtagtatccg tctcagttcg tggcgcaaaa tgagaaacca
     3541 ataagaactc aaaatgaacc acaacaagaa cataaagaga ctctaaaaga gcaacaccgc
     3601 ttgacataga ctcaaaatga gtcacaataa gaaacaataa aaaactcaaa aagatgcacc
     3661 gacataaagg atgctcaatt tgagataaac acaaactcaa aacgaggcaa aaagaatgct
     3721 aaaagtatca tgcgatgatg gttcaactaa cgtaaaactg gcctggcttg aagatggcga
     3781 ggtaagaacc agtctgtcag gaaacagctt taaagaaggc tggaacccgg gactgtttaa
     3841 tgccggcaaa gtttacaatt acgtcgtgga tgaaaaaaaa tacacctatg atttgggaag
     3901 cactgctatc ataggtacaa cacatgtcag ttatcaatac agcaccacca atctgcttgc
     3961 catacatcat gcactgctga catccggcct tcagccacag gatgtagaac tgacagtaac
     4021 attacctgtg accgaattct ttgataatga caatcaacca aatgaagaaa gaatagaaag
     4081 aaaaaaggca aatgttctcc gtgagatatc tctgaataaa ggggaaacat ttaaaattaa
     4141 aaaagttaat gtaatgcctg aatctttgcc agctgcattt gaatcactaa aaaaagataa
     4201 agtaaacaaa cttgaacgca gcctgattat cgatcttgga ggaaccactc tggattgtgg
     4261 tttaattctc ggtgcatttg aaggcatttc tgaaatccgt ggctattcag aaatcgggac
     4321 atcccgcatc actcataccg tcatgaatgc gctgacaaaa gcctccacac catgtaatta
     4381 ttttattgct gatgagttaa taaaaaacag acacgataat gaatatctcc agacattaat
     4441 aaatgatgta gctgaaataa aaaacatttc tcatgtaata gacagagaag tgaaatctct
     4501 ggcagaaagc atcaggcagg aaatttcaac attctcagga atgaaccgaa tctatctcac
     4561 tggcggagga gccgagctta tttaccctca cataaagcaa tatttcccga atctgaaagt
     4621 gaacaaggtg gatgaacctc agttggcatt ggtgaaagca atggtgcatg cgtaatggaa
     4681 agcagtgatc caaaaaaaag aaagaaagtt gtggcatacc tgcacccggc actttatccg
     4741 caggacaacc tgacacaaca aacaatagac tctctcccag tacaaatgcg gggagatttc
     4801 tacagacaat cccttatttg cggagcagct ctttattctg ttgacccccg actgttaacg
     4861 ctaatcagcg gttttttcag tgagaaaatc actgcagaaa atctggtgaa acttatagaa
     4921 caaacaacag gatacacatc cacatcaatt gatataagtg tgttaaaaaa catcatagag
     4981 gcatcttcag aaaacaaatc agagagcatc acttcaaaag atgatttcga agaacaaacc
     5041 agacgcaatc tttctatgtt aaaaaaataa taaaagaaga gcaggttcag tcaattacct
     5101 gctcttcttt tatacatact aaaaaatcag atttttacga atattcagat tatcataaaa
     5161 aatgcaacag acaggaatgc aataattttg cattatctcg ttgcttgtaa tgcaaatatg
     5221 cattattgtc agtgtgtgac aataacagag agatgacgat ggcgaccatt aacgcacgta
     5281 ttgatgatga tataaagaac caagcagatg aagtgctgaa gctgatgaat atcagtcaaa
     5341 ctcaggctat tgcagctttt tatcagtata ttactgaaca aaagaaactg ccatttgtga
     5401 tcacctcaat tgtaaaaacg ccgcatgatt tattacgtga aagtacagac atgttagctg
     5461 aagctcttgc agtgatttcc aatctacagg tgtggactga acagcaggat gggattggaa
     5521 aagcaaagct aatggagtat taccgccgc
//
LOCUS       DADLSB010000217         5445 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.217, whole genome shotgun sequence.
ACCESSION   DADLSB010000217 DADLSB010000000
VERSION     DADLSB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.217"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..185
                     /locus_tag="JN331_003842"
     CDS             <1..185
                     /locus_tag="JN331_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7261834.1"
                     /translation="GMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNI
                     NAVAMKITADIMQHAD"
     gene            complement(339..1988)
                     /locus_tag="JN331_003843"
     CDS             complement(339..1988)
                     /locus_tag="JN331_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY7261835.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2139..3488)
                     /locus_tag="JN331_003844"
     CDS             complement(2139..3488)
                     /locus_tag="JN331_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7261836.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTVFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFVTFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4194..4658)
                     /gene="soxR"
                     /locus_tag="JN331_003845"
     CDS             complement(4194..4658)
                     /gene="soxR"
                     /locus_tag="JN331_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY7261837.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4744..5067
                     /gene="soxS"
                     /locus_tag="JN331_003846"
     CDS             4744..5067
                     /gene="soxS"
                     /locus_tag="JN331_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY7261838.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5070..>5445)
                     /locus_tag="JN331_003847"
     CDS             complement(5070..>5445)
                     /locus_tag="JN331_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7261839.1"
                     /translation="MTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSF
                     VETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKA
                     MPPQVFMQWMEQLPARELTRGQ"
BASE COUNT         1460 a         1525 c         1309 g         1151 t
ORIGIN      
        1 agggaatggg catttccatt ctccgtgggg aggaaaatat ccccattgat agtgataccc
       61 tggtaacact acagaaattc tttgaagaag attgtacctc tcatactggc atgtcacaaa
      121 ctgaggataa tattaatgca gtcgctatga agattactgc agatattatg caacacgcag
      181 attgaggtta agtcgaagaa cagccaatga attgacttta caagagagat aacttatttg
      241 ttttagaatc atgaatgttc gcccgagcta acctgttaaa ggtgcaaaag attgcacctt
      301 tttcagatta cctcgggcct ggttacgtcg ctttcggctt actgattttc ctcaattagc
      361 aacgcttcaa gcaaatcgag atcgtgcagt aatttttgca gcgtttcgtt gctgatctcc
      421 cgcgtggcgc gcaggtggta cagttcagca cgttcagaac gcaatgccgc cagacggaag
      481 cgacgctcaa ggttctcttc ctgcacggaa ctttcaacgt cgttacgtcc atcggcgcga
      541 cgacgcaggt taccaatgac gcgagaactg acctccgtaa gcaactggtt atcgatattc
      601 tcttcggtat ccgccgccag acgctcctcc attttctgga tcgccacaat cgccacttct
      661 gccgttgccg ctcgcgcaat acgttcttct ttcaattgtt gcgaatggtc cgcgacttca
      721 atgtgttgta gcagaatagg caacatcacc acgccgacaa acagcgagaa gagaatgacg
      781 ccagccgcta ggaacaccag ctcatagcgc gccgggaaga cgttaccatc cggcaagagc
      841 agcgggattg agagcacacc ggccagagtg atcgccccac gcaccccagc gaaagacgcg
      901 atcaggattt ctcgtgtggt ccacgaacca aactccatcg gcttcttctt caggaagcgg
      961 ttgctgaact ttttcatcgt ccacagccag ccgaaacgga ccagcatcag cgccgcatat
     1021 atcagaataa tatcggtaaa cagcatccag atttcgacgt tagggtcgat ttctgccgcc
     1081 atcagcgacg tctccagaat acccggcagt tgcagaccta acagcaggaa caccatgccg
     1141 ttaaagacaa attccagcat cgcccaggtg ctgtttgcgc gcaggcgcat tgccagtggc
     1201 gcacggcgca tcacaccgga gcgggtgatg gtcatcccgg cggcaaccgc agcgaggatg
     1261 cccgaaacgc caatatgttc ggcaatcaga taagaagcga atggcagcaa gaacagcaga
     1321 acgatctgcg tcgcgggttc atcaccgccc cagcggctga ggaatcgcag cgagcgacca
     1381 tacagccagc tcaccacaaa accggcaaga ataccgccaa tggctacttt cataaattcg
     1441 accgtcgcac cgccgacagt gaagatcatc gtccccatcg ccactgccac ggcaaacttc
     1501 aacgacacca ggccggaagc gtcattcatc aacgcttcgc cctgcaaaat gcccatgatt
     1561 tttttcggga tgcgcccttc gccaacaatc ccggagagcg ccacagcatc ggtcggagaa
     1621 agtaccgccg ccagcgcaaa ggcggggatc agcggaatgc ccggcaccac ccagtaaata
     1681 aggaagccaa tgccgaccac ggtgaccacc accagcgcca gcgcgaggcc gaaaatctct
     1741 cgaccatgtt cgagaaattc acgggtcggc gttttccagc catcagcgaa cagcaacggc
     1801 gggataaaca agactaaaaa gagttcagga tcaaactcca catgcaaacc aaacgtcggc
     1861 cacgccagta gcgcaccgat ggcgatttgc ataagcggaa gcgggatctg aaagggcatg
     1921 acacgagtga ccaccccgga cagcgagacc acgagggtca ttatcagtat ggtgaagaag
     1981 atttccatgc gttccctgtt tgcgatgttt tttatgatct gagaaagacg ttatgcctga
     2041 attctcattt tttcagaata tcgcaaaagc tcacacaatg tgcggagaaa ataaaaacgg
     2101 gcagccattg ccacccgttt tcagaatccg atgaaagatt agatagccca gccacccgca
     2161 tagaaggtca ccagcgccac ggcgataacg accgtaccga tgttcaactt gcgccattca
     2221 ccggaaacca gacgaccaat caccagagtc gcgaagccga tcatgatgcc tgttacgatg
     2281 ttacaggtca gcacgatgaa taccgccgta accagacccg ccatcgcatc aacaaaatca
     2341 gcaaagtcga ttttcgccac gttgctcagc atcagcaggc caacgtacat cagcgccgga
     2401 gccgttgcat accccggaac gaggtaagaa agcggagaga ggaacagaat cagcaggaac
     2461 agcacgccaa cggtgatagc cgtcaacccg gttttaccgc ccgccgccgt acccgccgca
     2521 gactcgatgt ataccgctgc cggagctgca cccaccaggc cagagaaaac gctgctcatg
     2581 gagtcagtgg tcagtgcttt cccgccgtcg atgatctgcc catctttatc cagcaggttc
     2641 gcctggccgg cgacagcgcg gatagttccg gtggcatcaa atactgccgt catcaccagc
     2701 gccagaacgc ttggcaggac tacaggattc agcgcgccca taatgtccag gctgccaatc
     2761 agtgaattgc cgttttcatc gctcaatgaa ggcatggcga aaacgccgga gaaatggacg
     2821 ttaggatcga agatcaaacc gacaattgag ataccgataa tggtcagcag aatgccacca
     2881 gggactttca gtttttccaa gccgatgatc accgccagac ctaccagtga cataatcacc
     2941 gggaaggtca cgaaatcacc cagcgcaacg ggcagaccat caagcgggtt tttaatcacc
     3001 agaccgacac cattagcggc aatgagcagc aggaacagac cgataccgat ccccgtgccg
     3061 tgcgccacac cgtgcggcaa gttgcgcaaa atccagctac ggatacccgt ggcagaaatt
     3121 accgtaaaca gcacacccat caggaacacg gcacccagcg cgacaggtac gctaatatgt
     3181 tgccccagca ccaggctgaa tgcggtaaac accgtcaggg agatggcgca accaatcgcc
     3241 aacggcagat tagcccacag acccatcacg atagaaccga gtccggcaac cagacaggtt
     3301 gcaacgaaaa ctgccgcagg cgggaagcct gctttaccca acatacctgg aacgacgatg
     3361 accgagtaga ccatcgccag aaacgttgtt aacccggcaa ccacttcctg acggacagtg
     3421 cttccacgtt gtgaaatttt aaaccaggcg tcgagtgaac cgccggtacg cgctgatggc
     3481 gtagacataa gaaaacatcc cctgagagtt taattttcgt caagatgcgt ggcggacaat
     3541 cccctgccag cgaaacgtta tcttcctgct ccaggtttgc ggcaatttgg tgcgtcgcaa
     3601 aaaagcaaac gtttagctcc gcgcatacaa aacgggtggt aaaaaaaagc aaacgattat
     3661 ccggcgatca tagcggcttt ttcaaccgaa gtttgctgct tttttctctt ttattgtctt
     3721 gacgatgaaa aaccaggcaa ttgatgcgca aacgttatcg tcgatgcgca agattgcagc
     3781 gttatcgagc cgtataccgt tctggattgc gataaaaaat cgtctgagat gacaaaaata
     3841 ttgcggctaa aaagtatact tttacactgg cgaagaaacg tcggtgacat acaattaaag
     3901 catcaacacc aaccggaacc tccaccacgt gctcgaatga ggtgtgttga cgtcggggga
     3961 acccctcctg tgttccagcg ggatagagag aaagacaaag accggaaaac aaactaaagc
     4021 gcccttgtgg cgctttagtt ttgtgtttgc ggataattcc aaaagagctc gatcagtccc
     4081 ctcgcccctt cggggagagg gttagggtga ggggaacagg cagcaccagt gcaaatattt
     4141 accctcaccc cggccccata gttaacgatg agtcagcttg tccttgtggc gctttagttt
     4201 tgttcatctt ccagtaagcg tgcgccggta ccttcttctc ctaagcggtc gcccgggtta
     4261 cgcaacgggc aatcactgcg cgaaaggcag ccacaaccaa tacatccgtc cagttcgtca
     4321 cgcagcgcca ctaaggtatg aatgcgccga tccaactctt ctcgccattg ggacgaaagc
     4381 tgtttccact ctttcgcact taacgtatgc ccttctggca acacgccaaa cgcttcacca
     4441 atggtcgcca gcggaatgcc aatacgctga gcgattttga taattgcgac atatcgcaac
     4501 acatcacgtt tatatcgccg ctgattgccg ctgttacgga tactagtaat caaccctttg
     4561 ctttcataga aatgcagcgc cgataccgcc acaccgctgc gtttcgccac ttcgccaggg
     4621 gttagcagcg ctttaatgcg gggtaatttc ttttccataa atcgctttac ctcaagttaa
     4681 cttgaggaat tatactcccc aacagatgaa ttaacgaact gaacactgaa aagaggcaga
     4741 tttatgtccc atcagaaaat tattcaggat cttatcgcat ggattgacgt gcatattgac
     4801 cagccgctta acattgatgt cgtcgcaaaa aaatcaggct attcaaagtg gtacttgcaa
     4861 cggatgttcc gcacggtgac gcatcagacg cttggcgatt acattcgcca acgccgcctg
     4921 ttactggccg ccgttgagtt gcgcaccacc gagcgtccga tttttgatat cgcaatggac
     4981 ctgggttatg tctcgcagca gaccttctcc cgcgttttcc gtcggcagtt tgatcgcact
     5041 cccagcgatt atcgccaccg cctgtagttt tattgcccgc gcgttaactc ccgcgcgggt
     5101 aattgctcca tccattgcat aaacacctgc ggcggcatcg ccttcgcaaa aaaccatccc
     5161 tggcaatagc gcacgccgcg tttgcgcagc cagttaacct gctcctcagt ttcgacgcct
     5221 tcagcgatcg tttttaaccc caggctgtgc gccagctcga tgatgtgttc cgcaatcaaa
     5281 tgactggttt tgtgggtggt cagcgtttca acaaacgatt tatcgatttt caaaatatcg
     5341 acattcaatg atttaaggtt atgcaagtta gagtagccaa taccaaaatc atcaattgcc
     5401 acttcgtaac cagcctggcg gaaagccaga ataatcggcg tcatt
//
LOCUS       DADLSB010000218         5353 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.218, whole genome shotgun sequence.
ACCESSION   DADLSB010000218 DADLSB010000000
VERSION     DADLSB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.218"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            253..510
                     /locus_tag="JN331_003848"
                     /pseudo
     CDS             253..510
                     /locus_tag="JN331_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(560..1510)
                     /gene="uspE"
                     /locus_tag="JN331_003849"
     CDS             complement(560..1510)
                     /gene="uspE"
                     /locus_tag="JN331_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY7261840.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1662..2414)
                     /locus_tag="JN331_003850"
     CDS             complement(1662..2414)
                     /locus_tag="JN331_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY7261841.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2609..3124)
                     /gene="ogt"
                     /locus_tag="JN331_003851"
     CDS             complement(2609..3124)
                     /gene="ogt"
                     /locus_tag="JN331_003851"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY7261842.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3135..3800)
                     /locus_tag="JN331_003852"
                     /pseudo
     CDS             complement(3135..3800)
                     /locus_tag="JN331_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3897..4925
                     /locus_tag="JN331_003853"
     CDS             3897..4925
                     /locus_tag="JN331_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7261843.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKLRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKLGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            4922..>5353
                     /locus_tag="JN331_003854"
     CDS             4922..>5353
                     /locus_tag="JN331_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAY7261844.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLS"
BASE COUNT         1380 a         1206 c         1302 g         1465 t
ORIGIN      
        1 agcgatcatt gttttatcag gctcctccag ataattgtcg tcatgccgga aacccctggc
       61 ggggctattt taccgcgaca attcattcag atcatcaata gtcagggaag gaagtagcaa
      121 cattagctaa ctaatcaatg cgttgattgt aaatccagct aagaggtgag gttttcagag
      181 cagacaacgg tgaaacgtca tggtattgtt acgtttaggt aacaagaaat ttgtctgcac
      241 aaggattaca tcatgattat ggcgaaactg aagtcagcga aagggaagaa atttctcttt
      301 ggtttgttgg cggttttcat tattgcggcg tcggttgtga ctcgcgcaac catcggcggc
      361 gttatagaac agtacaatat tccgctgtct gagtggacga catcaatgta tgtgatttag
      421 tcatcgatga tttttgttta tagcctggtc tttactgtgt tgctggcaat cccgttggga
      481 atttatttcc ttggcggcga agagcagtaa gtaaaaaata ggcccgataa ctcgggcctt
      541 gtcagttatt gaagagtcgt taatcgtctt cttcgtcatc cagttcaacg ggtgtctgat
      601 actggtcagg tttaatgacc agcaggtcgc agcgaagatg atcaatcacc tgttccgccg
      661 tgttgccgag gaatgctgct gaaataccgg tgcgtccgac cgtgcccaga accacaattc
      721 ccgcctgtaa atgctccgcc aaatcaggaa tcacctcttc gggcagacct ttttctacgt
      781 gcgtcatgtt ttcattaatg ccgaatttct gccgcagggc tttcattgcc agcaaatgtt
      841 gcccacgaat ggcatcgtta taaacgctcg ggtcaaattc cggcagttca atcgcgatat
      901 taattggcgt taccggataa gcgccaacca gatgaacttc ggtatggttg acttgttctg
      961 ccagttcgat cgtctctttg accagttttt cattgagcgc attatgatac ggctcttcac
     1021 tggcgagatt caccgccacc agcgccttgc ctccttccgg ccacggctgg tctttcacca
     1081 tccacaccgg gcttgggcat ttgcgtaaca gatgccagtc cgttggcgta aaaatcaccg
     1141 cttccagacg gtcatgttgg tgcgccattt ttagcaccaa atcgtgtccg ccgctgatca
     1201 cttcctgaat gatggcttcg aaaggacggt tatgccagac cactttaatt tcaatgggaa
     1261 cgccagcatt gagataatat tttgcctgct cgtggatcca ggctgtacgc tggctgatga
     1321 cgccctgacg catagcggta cgttcgtccg gggagagcag ggtggtcatt tcgtatgaga
     1381 agtcatagat cggcaaaaag gctttaattt tgccaccaat ccgttgatgt aaataaacag
     1441 ctcgccgcaa tgctggttgg tcgtcctggt taggatcgat aacaacgagc atgttctgat
     1501 acatagccat acagggtctc cttacaacaa ctgtcaacgc agtttgtaat taaaagatta
     1561 acccatatct ggtgaatgaa acagtgatga accttctgcc agatcaataa atcagaaaaa
     1621 tttaatgata tgacagaagg atagtgagtt atgcggaaaa atcaggcaac gttacgcgta
     1681 tgaccagcaa gctgggccag cgcatcgtta ttttcgatgg tgatgtattt acctttgact
     1741 gccagcatac cgcttttctg gaagcgaccc agcagacggc tgatggtttc taccgtcagg
     1801 cccagatagt taccaatatc gccacgagtc atcgtcaggc ggaattcacg aggggagaag
     1861 ccgcgttggg caaaacgacg ggacaggttg tagatgaatg cagccagacg ttcctcggca
     1921 tttttcttcg acaacagcaa gatcatgtcc tgatcgcctt tgatttcacc gctcatcaga
     1981 cgcatcatct gctgacgcag attcggcatt ttaccggaca aatcgtccag cgtttcgaac
     2041 ggaatttcac ataccatcga ggtttccagc gcctgagcga agctcgggtg atggccgctg
     2101 ccgatggcgt caaatcccac taggtcgcct gctaaatgga aaccagtgat ttgctcgtcg
     2161 ccttgctcag taattgtata acttttaatc gtaccggagc ggatggcata aagcgattta
     2221 agttcatcac cagccttaaa cagcgtctgg cctttctgaa taggcttctt ccgctcaatg
     2281 atattatcaa gctgatcaag ctcatgttcg ttgagtgtga acgggatgca aagctggctg
     2341 atgctgcaat cctggcaatg gatagcacaa ccgccagact gaatgcgccg tataattcgc
     2401 ttttccggga tcataggtct gctcaagccg taattgatat ttgtcaattt taacatcttt
     2461 ttagggagca agtaagtcta agcaaacctt aacagcagag aattccgata ttagatgtaa
     2521 atatatgtct atctatttga aactccttaa gttgtcaagg gtaactttac ataaaagtgt
     2581 gaacaagctg gcacaaattg tttaatgttt acagcaaaag ataaccttcg tggcgcaata
     2641 accactcttt tcgctgaact ccgcctgcat atccggtcat ggtgccgttt cggccaataa
     2701 cccgatggca aggtacgacg atgctgatgg gattcgatcc gtttgccgca ccaacggcac
     2761 gcgccgcgcc agggcggccc aattgttcag ccagttggcc gtaatgcatt acctgcccgc
     2821 aggggatagt gcgtagtgtt ttccagactt cgcgctgaaa tggcgtcccc cccgtggcag
     2881 tgggaagcgt atcaataatg ctaagattac cggcaaaata ttcacgaagc ttgtcgctta
     2941 aaccacctgg gttggtggca gaaatgcgct catagccttc tttgcgataa tggatgtcca
     3001 gcagctgcac catgcgttcg ctgtactctt cccattcaac cgcccgcagg cgaaattgct
     3061 catcgcaaat cacccacagt ggacccagtg gcgtggcaat tttttcttca agtaatctca
     3121 gcatccgttc tctctccatc atcaagaagc gtcactgcag aaataacgtg catttgctga
     3181 acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag tgtacggcta
     3241 ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg gttgtttagc
     3301 aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc aagtgacatt
     3361 ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa acgtaactgt
     3421 tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag taattccctt
     3481 aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact ttccgcttct
     3541 gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg cggcatttgt
     3601 gggggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc agcccataca
     3661 cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg ccagggaact
     3721 gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg tgatgctttg
     3781 ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca aataagccat
     3841 gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa agagtgatga
     3901 taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg actggcgttg
     3961 tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat gatgcttaca
     4021 ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct gctatagagg
     4081 tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat gatcgggatt
     4141 atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc aatctcgaag
     4201 gcattcaagc cactattacg atgcagcagt cgataattca aagtgccagt gaaacatggc
     4261 aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaacgttat gaaaaactcg
     4321 cacaatcagc ggcaatatcg caacaaatta ttgataacgc caggtttgac taccagcagg
     4381 tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt ggagaagcta cggctggcag
     4441 ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga agaagttcag gccgctttga
     4501 ctcaggcact actggatctg gaatataccc ttgttcgtgc gccgattgat ggcattgtcg
     4561 caaaccgcag cgcccataca ggcagttggg tggaaggcgg cacatcgtta gtttcactag
     4621 taccagtcag tgagttgtgg gttgatgcaa attataaaga aaaccaaata gcgggaatga
     4681 agctaggaat gaaggctgag atccgtgctg atattctcaa aggggaagtg tttcacggtc
     4741 acattgaaag cctttcccca gcgacaggag ccagtttcag cttgattccg atagaaaatg
     4801 ccactggcaa tttcaccaaa attgttcagc gtgtcccggt acggattgca tttgacgacg
     4861 caaaggaatt aaagcaactt cttcgcccgg gactgtccgt caccgtcagt gttgatgaga
     4921 gatgatgtct atgcgtaaac atattgcttt cgccagtatg tgtatgggat tatttattgc
     4981 tcagcttgat attcaaattg tatcgtcatc cctaaatgaa ataggtggtg gtctgtccgc
     5041 aggtaaagac gaaatggcct ggcttcagac cagctattta attgctgaga ttattgttat
     5101 tccgctctct gggtggctgt cacgggtatt ttccactcgt tggcttttta ctctatccgc
     5161 gggaatattt actttaatga gtatcgcgtg tggacttgca tggaatattc agatcatgat
     5221 tttgtttcgt gctttacagg gagctgctgg cgcatccatg atccctttag ttttcacgac
     5281 ggcgtttatt tattaccagg gtaaagagtt ggggttggct gctgcggtag tcagtgcgct
     5341 ggcctcactg tca
//
LOCUS       DADLSB010000219         5333 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.219, whole genome shotgun sequence.
ACCESSION   DADLSB010000219 DADLSB010000000
VERSION     DADLSB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.219"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..232
                     /locus_tag="JN331_003855"
     CDS             <1..232
                     /locus_tag="JN331_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAY7261845.1"
                     /translation="VVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYP
                     HQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            242..1573
                     /locus_tag="JN331_003856"
     CDS             242..1573
                     /locus_tag="JN331_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HAY7261846.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGSGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGLHNGMMLDDAFFGCARNADA
                     IIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGGFLEIAQNARKVV
                     FCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQSGQEVLYITERA
                     VFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFTPMEDA"
     gene            1570..3642
                     /locus_tag="JN331_003857"
     CDS             1570..3642
                     /locus_tag="JN331_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAY7261847.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSKLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQHGRRVFMDFNRNPEAVPGDLPFTLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNSGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVIAVRLARFIGCTQKRSVEGDVAQLVASS
                     LNIVREVIAQALVNENGMALVTVKEKIQARMSDYAGFICHADKVRHATRDALLLSEFV
                     QQHGLTINHAGEITELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIVIDPQGKCLP
                     QTRRGAKEEWRFRSERAEDKNHRLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LNGEIYTQ"
     gene            3735..5012
                     /locus_tag="JN331_003858"
     CDS             3735..5012
                     /locus_tag="JN331_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7261848.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPVMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATH"
BASE COUNT         1326 a         1260 c         1377 g         1370 t
ORIGIN      
        1 agtggtagaa aaagaacagg tggaaagctg gatggcgaac cagttagcgc agctggaaaa
       61 atgcggtccg gtggcactgg cgcatattaa gcaactgatt ctggcactgg aaaataaaca
      121 tgccgattac ccgcaccagc tactggccgg attaatgtct gccactcagg attgccagca
      181 ggcgacgcgc gcctttgccg aaaaaagtag cgtgagcttc cataatcaat aaggaaattg
      241 tatgcgtaag ataacaaccg ccgaggcact ggcggcgcaa attcaggacg gtgcgactat
      301 tgctattagc ggtaacggcg gcggtatggt agaagccgac catattctgg ctgctattga
      361 agcgcgtttc ctgcaaaccg gacaccctcg cgacctgacg ctaatccact cgctgggtat
      421 tggcgatcgc gactgcaaag gcactaaccg ttttgcgcac gcagagatgc tcaaacgcat
      481 tattgccggg cactttacct ggtcgcccaa aatgcaggcg ctggtaaaaa ataacacgat
      541 tgaagcctac tgctttcctg gtggcgtgat tcaggcactg ctacgggaga tcggctccgg
      601 acgtccgggg ctttttaccc acgttgggct gggatcgttt gttgatccac gcaatggcgg
      661 cggtaagtcg aatgaatgca ctaccgacga cctggtagaa ctgattgaaa tcgacggtga
      721 aaccaaactt cgttatcgcc ctttcaaagt ggattacgcg attttgcgtg gcacttatgc
      781 cgatcctcga ggcaacgtca gccttgaaga agaagcgatt gatatggata gctattccat
      841 ggcgctggca gcacacaaca gcggcggcaa agtgttcgta caggtacgcg atgtgctgga
      901 agctggcgct tcaaccaact ttggctttgg tattcctggg ggtattccag gcgtagcgct
      961 gcgcgaaggc gtgccttacc aaagtttatg gctgagcgtc gagcaaggcc tgcataacgg
     1021 tatgatgctg gatgatgcgt ttttcggctg cgcccgcaac gccgatgcca ttattccatc
     1081 actcgatcaa tttgagttct acagtggcgg agggatcgat atcaccttcc tcggcatggg
     1141 agagatggat cagtacggta acgtcaacgt atcccatctc aatggcaatc tcattggtcc
     1201 cggcggtttt ctcgaaattg cgcaaaacgc ccgtaaagtg gtgttctgcg gcacgttcga
     1261 cgccaaaggt agcaagattg atgtaacgcc agatggcttg catatcaccc agtcaggtca
     1321 aatccctaaa ctggttaccc aggtggaaaa aatcactttt agcgccgcct acgcacagca
     1381 aagtggtcag gaagtgttgt atatcactga acgcgcagta ttccagttaa cggcagaagg
     1441 cgttgaatta attgaaatcg cgccgggtgt ggagattgag cgcgacattc tgccgtatat
     1501 ggctttccgt ccaattatca agcacccacg cctgatggaa agtagcctgt ttacgccgat
     1561 ggaggatgca tgagtcagct tgatgacacc atccttgatg cgctgacgca cgttactttc
     1621 ccaaagggtt ttgcacaggc agagcccgca tgggttgtca cggtagacgg tgttgattac
     1681 ccactctggc aaacagatgc cctggtagtc ggcagtggtg cagccgggct gcgtgcagct
     1741 gtggaactta aacgccgcca gcaaaatgtg ctgatcgcca ccgccgggtt atatatgggg
     1801 acgtcggcat gttctggttc cgataaacag acactgttta ccgccgctac cgcgggcaac
     1861 ggcgacaact tcaccaaact ggcagaggca ctggcgagcg gtggggcgat ggatcacgac
     1921 accgcttatg tcgaagcggt aggttctctg cacactcttg gcgggttgca atatcttggt
     1981 ctggaattac cggaagatcg ctatggcgcg attcttcgtt atcaaaccga ccatgacgaa
     2041 gccgggcgtg caacctcgtg tgggccgcgt acctcaaagt taatggtgaa agtgctgttg
     2101 gaagaagtac agcgcctcgc cattccagtg ttgaccagtg caacagtgat taaactgctg
     2161 catcagcgtg acgaaaacgg cgaagaccgt gtggcggggg caatcctcgc gaccggtcat
     2221 cgcgcccata acccttgggg gctggcaatt gtgactgcgc ccaatgtggt actggcaacg
     2281 ggcggaccgg gcgagcttta tcgcgacagt gtttatccac ataaatgctt cggctcgctg
     2341 gggctggcgc tggaggaagg tctgacgcta accaatctga ccgaaagcca gtttggtata
     2401 ggcacaccgc gcagcacgtt tccgtggaat ttgtccggca cctatgtgca ggtgatcccg
     2461 tatatctatt ccgtggatgc tgagggtaac gagtataact tcctcgcgga ttactatcgc
     2521 accacccaag agctggcttc aaacatcttc cgtaaaggct atcagtggcc gttccacgcc
     2581 acgcgggtaa tggattttgg ctccagcttg ttagatatgg cggtggcgca agagcagcaa
     2641 catggtcgtc gggtgtttat ggatttcaat cgcaatcctg aagcggtgcc tggtgatttg
     2701 cccttcacat tagatcgact ggacgacgac gttcgcgcgt atctggaaaa taatgacgct
     2761 ctggcaccat cgcccattga acggctgcaa cgaatgaatc cgctgtctat ctcgctgtat
     2821 aagatgcacg gttacgatct caccacgcag ccattgcagt ttgccatgaa taatcagcac
     2881 atgaatagcg gcattgaagt ggatatctgg ggacaaacat ccctgcccgg ttgttttgcg
     2941 gtgggagaag tagccggaac tcacggcgtt acccgaccag gcggtgcggc gctgaatgcc
     3001 ggacaggtta ttgccgtgcg tctggcgcgc tttattggtt gcacacaaaa gcggtctgtt
     3061 gagggtgatg tcgcgcagct ggtagcctca tctctgaata tagtcagaga agttatcgct
     3121 caggcactgg taaatgagaa cggaatggcg ctggtaacgg tgaaagaaaa aattcaggcg
     3181 cgaatgtctg attacgccgg atttatttgt catgccgata aagttcgtca cgccacccgt
     3241 gacgccctgc tgctaagtga gtttgtacaa caacacggtt taaccatcaa tcacgcaggc
     3301 gaaattaccg aactatttat gtggcggcat atggcgctga cttctgccgc cgtattaacg
     3361 caattgacgc attatattga cgcaggtggt ggcagtcgtg gggcacggat agttattgat
     3421 ccacaaggca aatgcctacc acaaactcgt cgcggcgcaa aagaagaatg gcgttttcgc
     3481 tctgaacgtg ctgaagacaa aaatcacaga ttaacgattc aatattcgca aggttctttt
     3541 attaccgaag tgaagtcttt acgtatgcaa ccgtgtatta acggtattta ctttgaaaaa
     3601 aactggccag actttttaaa tggagaaatt tacacacaat aattttcaat atttcctctg
     3661 tacctgattt ttattaccta ttacacggag acaccaacaa ccagggttta ttaatataat
     3721 taaaaggttt aaacatgaat aaaataaaga attaccggtg gcatatgatt gccctcgtat
     3781 gctttatcac tgtaatcaat tatctggaca gaacggcgtt aggtattgcg gctccaacga
     3841 ttatggagac aaccggaata actaaagagc aatattcatg gattgtcagt gcattccagt
     3901 tggcctatac attagggcaa ccggtaatgg gcttctttat tgataccgtg ggtctgaagt
     3961 taagttttgc gatatgtgcc gcaatttggg gcctggcgac aatgggccat gcactaaccg
     4021 gaacgtggtc tggtctggca tttatgcgcg ccctgatggg tttcagcgaa gcgtcggcaa
     4081 ttccggcggg tgtaaaaacc gcatcaacat ggttcccggc aaaagagcgt ggcgtggcga
     4141 caggtgtttt caatatgggc acctcactcg gcgcgatgct tgctccaccg ttgattgcct
     4201 ggtgcattat gtttcatagc tggcaatttg cgtttattgt ttcaggtagc cttgctttgc
     4261 tcgcggcttt attttggttc ttttgttata aagatccgaa agatgccaaa cgcctttctg
     4321 atgaagagcg ccactatatt gaatcaggac aagaacagca tcttaaaaca gataagaaag
     4381 aaaaaacgtc aatcaagcat atcctcagcc aacgtaattt ctgggggatt ggcatcgcgc
     4441 gttttctcgc agaccctgca tggggaacca ttaacttctg ggtgccgatt ttcttcgtcg
     4501 aaacgctgca ttttagcctg aaagaaattg ccatgttcgt ctggctgcct ttcctgctgg
     4561 gcgatctcgg ctgtttagcc agtggttttg tcgcgaagtt cttccacgat cgcggcgtga
     4621 gtttaattaa ctcacgaaga attaccttca ctattgcagc cgtcattatg atgacgattg
     4681 gcctggtgag tattgtcgaa aatccctaca ttgccgtatt actgattagt attggcgcgt
     4741 tctcgcatca atgtctttct actgtagcag caactctggg tggcgatctg ttcaaaaaag
     4801 acgaagtagc taccgcagtg ggtatggcag gagcctgtgc gtggagcggt cagttgattt
     4861 tcaacctgtt catcggggca ttcgttcaca ttatcggctt cgcgccgttc tttattgccc
     4921 tggctttctt tgacattatt ggtgctattg cgttgtggac gcttatcaaa gttaaagatg
     4981 aagaaccaca agtacagtta gctacccact aattatttta aataaaaacg ctataattga
     5041 ggttatggcg tttttaaaga agcttgagtt atcgaacctc gcttaccaac gcgttgtcag
     5101 tcatctcccc cctccctggt gcatcgttat atccgtgatg agcgattttt gatattaata
     5161 tctttcacca tccgctattt tcaccacttc agaaaacaaa cttattttta ttaaaatcaa
     5221 ttctttttct ttgaacattc aactgcgata tttgtaaaaa cattaaacag gcaacaataa
     5281 aatgacatta ccaacaacta tttattcatt ccccgcatat ttaccccgtt ttt
//
LOCUS       DADLSB010000220         5289 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.220, whole genome shotgun sequence.
ACCESSION   DADLSB010000220 DADLSB010000000
VERSION     DADLSB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.220"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(57..476)
                     /locus_tag="JN331_003859"
     CDS             complement(57..476)
                     /locus_tag="JN331_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261849.1"
                     /translation="MHQLRHPLLGCVFCNECDPHPFAPDPLQIFHHYYEWVRPCFPIR
                     AGNRFSRSLRWSGPGSCCLHTGCRAVSKQVSSALIRGRLYGPILTSSKISMLHRTVYF
                     RSASRTPRDSRSLPFPESLTTSFLRMKQHRVVWSVLL"
     gene            526..1704
                     /gene="ltrA"
                     /locus_tag="JN331_003860"
     CDS             526..1704
                     /gene="ltrA"
                     /locus_tag="JN331_003860"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7261850.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1787..1966
                     /locus_tag="JN331_003861"
                     /pseudo
     CDS             1787..1966
                     /locus_tag="JN331_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1996..2478)
                     /locus_tag="JN331_003862"
                     /pseudo
     CDS             complement(1996..2478)
                     /locus_tag="JN331_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2734..3714)
                     /locus_tag="JN331_003863"
     CDS             complement(2734..3714)
                     /locus_tag="JN331_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY7261851.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3714..4913)
                     /locus_tag="JN331_003864"
     CDS             complement(3714..4913)
                     /locus_tag="JN331_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY7261852.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
BASE COUNT         1481 a         1192 c         1238 g         1378 t
ORIGIN      
        1 gcagcataag ttatccatgc tctgaggtgg gtggaggtgg gccctccggt cagggtttac
       61 aggagcactg accaaacgac ccggtgctgc ttcattctaa ggaatgaggt ggtgagcgac
      121 tcgggaaaag gcagagatct actgtcacgt ggggttcggg aagctgaacg aaagtaaact
      181 gtccgatgaa gcatcgaaat cttagatgat gtcaaaatgg ggccgtacag ccgtcccctg
      241 ataagcgcag aagacacctg cttactgact gcgcggcatc cggtgtgaag gcagcaggat
      301 cccgggccag accatctcag ggaacgtgag aacctgtttc cggcacgtat cgggaaacag
      361 gggcggaccc attcgtagta gtgatgaaag atctgtaatg ggtctggagc gaagggatgg
      421 ggatcacatt cattgcagaa gacacaaccc aaaaggggat gacgtaactg atgcaacgaa
      481 aatcatttga aataccaaaa gcactggtct gggcatcata tctggatgtg cgtcgcaaca
      541 agggagcccc ccggatgcga cgggcagact ttgaaaatgt tcgaccagca acgtgatggc
      601 aatctataca agatctggaa ccggctatgt tcgggaacat ggtttccgcc accagtgctg
      661 gaaaaacgga tcccgaagtc gaatggcaag gaacgcattc tggggatccc aacagtatcc
      721 gatcgaattg ctcagggagc gataaaactt ttcatggaag aaaagcttga cccgattttc
      781 catgctgatt catatggtta ccgaccaggc aaatcagctc atgacgctct gaaacaatgt
      841 gccatccggt gctggcgtta tagctggata ctggaagtgg acatcagtgc gtttttcgac
      901 catgtgagac atgatctggt gctcaaagct ctggaacacc acgggatgcc taaatgggtc
      961 atcctgtact gtcgacgctg gatggaagcg ccaatgcaga gttgtgaaaa tggagaatta
     1021 ataacccgaa cacgaggcac tccgcagggc ggggtcatta gtccgctact tgctaacttg
     1081 ttccttcact atgcatttga tttgtggatg gaaagagaat atcggggggt accgtttgag
     1141 aggtacgctg acgatattgt agtacattgt tcacgaatga gtgatgcaac aaggctaaag
     1201 aacaggttat cggagcgctt ctcggaagtc gggctggtcc tgaatgcagg gaaaacgaat
     1261 atcgcctaca ttgacacgtt taaaaggaga aacgtcgcaa cgagtttcac ctttctcgga
     1321 tatgacttca aagtgcgtac gctgaagaat ttcaaaggcg aacggtaccg aaaatgcatg
     1381 ccaggtgcgt caaatgcagc aatgcgcaaa ataacagaaa caatcaagaa gtggcgtata
     1441 catcgctcaa cagctgagag tttgctggat tttgcgaggc gctacaatgc gatagtgaga
     1501 ggctggatcg agtactacgg aaagttctgg tccagaaatt tcaactatcg actgtggagt
     1561 gcaatgcagt cacgtctgct caagtggatg cagtctaaat acagactttc gaaccggaag
     1621 gctcagcgaa agctgacgct ggtaaggaag gagtatccga agctatttgt tcactggtat
     1681 ttactacgtg catcaaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1741 tccgtgagca gccggggagt gaaattctct ccggctgact cgactcgcgg gttccgacag
     1801 cgatgacgaa catgcggcgg tgttgtactc gctgatcggc acatgccgtc tgaacaatgt
     1861 ggaaccagaa aaatggctgc gttacgtcat tgagcatatc caggactggc cggcaaaccg
     1921 ggtacgtgat ctgttgccct ggaaagttga tctgagctct cagtaaatat caatacggtt
     1981 ctgacgagcc gcttactttt ggcgtaattc tgctgcgcct ttactgaaca caatgccgta
     2041 ttcagcaaga agtccacgaa tgtgatttat cagttgtgtc ctttccattg aaaccaaccg
     2101 acgcatattc aaaaaagtct ggacgtcctg ttgctccaga gtttttattg gtacgggtcg
     2161 tatagttcca tgttggcagg cctcagctat tgcctgtgcg tcgttataat catttttctg
     2221 gcaccgctga taagctttaa catgttgtgc cggaagcaat attacatcat ggtcgagttt
     2281 cctgatttcc cgggcccagt aatgagatgc cccgccagcc tccatcgcaa cagtcatttt
     2341 aggctgttgc accagccagt taagcagttg attgcgagaa agtttggtct ttcctgcggg
     2401 tttgccatga gcattgatac tgaaaagata aaagttcgtt ttagccagat caatgccggt
     2461 gattttctgt tcagacatgg tatcccgctt acttttgtgg ttaaagttta tccatcatgg
     2521 cgcaattgaa ggctgccgtg aaagaggggg aagactatct catcggttac tgttaaacgg
     2581 ctaagagaaa ctatcttttt caagcaaaga agatacggtg aaacaggcga tttcaccgtg
     2641 aaatactgtt caaaaaaaac gctaataaaa gcaaaatttc ttttaaaaca gccaattaaa
     2701 aactggtgag ttgctggcga aaaacttgaa ttttcacagc gaattcagat tatcttttag
     2761 tacaagagca atcatacgat ctaacttatc ctgaacttcc aacggtaatc tgccgaattc
     2821 atatgagaac gtacgtccct ttactcgttt tcgagcaaat ttatctttac tatcaaattt
     2881 ccataacaaa gttacaaccg ctttatcctt tactcctgaa tcaagcaatg aagacatttc
     2941 cttggttata agccgcataa tactgttttt aacctcgtct tcagaaagat tgtcatcagc
     3001 agtaataagc tcaatactcg gcgagatatt cttcacaact tcatcaatag atatatcacc
     3061 cagcctcagt cgttcactta attcagccag ttgcctgtaa tcggcgtatg tcagctcaga
     3121 ctgaacagga aaaagagaca caaaatcttt aggtacggac gctgcttgga gggcacgagt
     3181 caccttagct gccgacaatc cctctctttc cgcaatttgc ttctggttca taccggcctc
     3241 tttgagacgt actaaacgca acccaatttc acgaatatta tgttcaagtg acgtctgcaa
     3301 atctttcgcc agatgctgag cttcagaaac agtaagctca tcatccgtta caagtacacg
     3361 taatccaacc ttgcatagca aagctgctgc ccgacgacgg gaaccatcca aaatctcaat
     3421 aagatcgcca gtccgaatcc caatacatgg atagaattgt tgtaaacgta tagttctggt
     3481 gatgtctttt agtgagtccg gcgtcagggc tgtctgttcg cggccattca cctcttcaac
     3541 cacaaaagtt tgagtgtcca ctttgtcatg atcaaccttg atctcagtaa attttgcctt
     3601 tcggccagta ttgagggtaa atacatgaac tggagccgat atttcttcag tattattcag
     3661 gatatttgta ttcagcgtac ggccaatagt cggtcggtgc tttctgtttt ccatcagtag
     3721 ttggccctta taaattcaat acgatcaaaa actgctttag cgaaatcttc tgctgccata
     3781 cgtgcatttt tcaaagcttc accactgcca acataagtaa caggattcgc agatatcacg
     3841 gtatcaaacg actcgccgct tctctcaaaa ccatcaagac gtggcattga aacatcaagc
     3901 atgtcaccac cgaaaatctc ttttgtcagg ctatggcagt atttatgatc tgacttattc
     3961 gccagtttcg acatgaagcc aatatttgcc tgcaaacggc atgaacatcc atcctgttca
     4021 ataatctgta caagttcagg taaacgagcc aaatatttaa gcgtagaatg gaaatcaact
     4081 tgtgctggcg gcaccggtgt aaacatgata tctgcggcag caatggcatt tttcaaaaat
     4141 gcgtccagat gcgggcctgt atcaataagg atgaagtcga aatcatgctt cagcttatca
     4201 ataatattct cacgcaatat cgcgtgtttg ttttgtccta atagatgctc ctcgcacaga
     4261 gtatcccagt ttgatgcaat aaaggcgtcg tcaatagaag ccggcataac ataaacgcca
     4321 gggataactg acggaacgat aaaatcttcc agtaattcct cacgactcac gttctgcaac
     4381 atagcctgcg gtgcggttgt atcaaccagc cccaccgcat gcaagtagtt cagaaacata
     4441 gtggctgatg actggggatc taaatcaagg agcagaatac gtaaatcttc tgacagcagg
     4501 tgaggatgag cacgcagggc gtgagctacg gaaacactac tcacggtttt agacacaccg
     4561 ccttttaaac taccgatgaa tatggaatat gcctcactat aacgatcgcg atatttagga
     4621 atgccacgat gtgcatagat atcgataatg ttctggatgg ttaatgcata ttttgtcgcg
     4681 gttcctgctg ggcgtttttc aaactgatag ccctgagcct ccatctcacc gactgcacca
     4741 tccacgcttg cgcgcgttaa ttttggaagc ttagctacag cagactttga atagacttga
     4801 taaaaaacat cagcctgaaa ctcctgcttt tgctcctgaa tctgttttgt tagggcgagc
     4861 aacattttat ctgcacgctg cgcaacctta cttaactgct caaaactagt cattccccct
     4921 ccgtgccatg taggatttta acttattaca aaaaaaactg cattagcagg gtaatgcatg
     4981 caagtacata ttgtacatgc ttatattaaa aatgcgcgca ttgctgattt attaatcatc
     5041 ggaatgaata ttactgtaag atagtatgcc tttcggggag aaagacgctg atttcccccg
     5101 aataatccag ttcaccacca cactccggac aacagctttc ttcaggctcc agatggtgta
     5161 tttcgcggga aacatgttcc ggtaacggac ggcgatgacg tgactgtcgc aacaggcggg
     5221 ggtagtgctt ccaatcagta gaacatgtgc ttttcaatga aggagccgat gactttttcg
     5281 tggatttcc
//
LOCUS       DADLSB010000221         5284 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.221, whole genome shotgun sequence.
ACCESSION   DADLSB010000221 DADLSB010000000
VERSION     DADLSB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.221"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..409
                     /locus_tag="JN331_003865"
     CDS             <1..409
                     /locus_tag="JN331_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7261853.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            429..740
                     /locus_tag="JN331_003866"
     CDS             429..740
                     /locus_tag="JN331_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7261854.1"
                     /translation="MTLTLNELLLKLDRYRVIVVDDLGYVKRDSAETGVLFELIAHRY
                     ERGSLVITSNHPFSMWGSIFVDETMAVAAADRLIHHGYMFELKGESYRKKTAKAVTSV
                     T"
     gene            complement(909..5003)
                     /gene="sepA"
                     /locus_tag="JN331_003867"
     CDS             complement(909..5003)
                     /gene="sepA"
                     /locus_tag="JN331_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY7261855.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSWADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1297 a         1329 c         1229 g         1429 t
ORIGIN      
        1 cgtcaggcag cagcgaagca aagcgttact gacgtcgctg aatgaatgga tggtggagaa
       61 gaatggtacg ctgtcgaaaa aatccagact gggcgaagcg ttcagctatg tactgaatca
      121 gtgggatgcc ctctgttatt acagtgatga cggtctggcg gaggcggaca ataacacagc
      181 agaaagagcg cttcgtaccg tctgtctcgg gaaaaagaat tacatgttct tcggcagcga
      241 tcatggaggc gatcgcggag ctctgctgta cggactaatc gggagctgtc ggttgaacgg
      301 tatcgatccg gaagcgtatc tgcgccatat tctgagcgta ctgccggaat ggccctccaa
      361 ccgtgttgac gaactcctgc catggaacgt ggttctcacc aataaataag cgtcaatacg
      421 gtgctccgtt gacgcttaca ctgaatgagc tgctactgaa actggatcgc taccgggtga
      481 tagtggtgga tgatcttggc tatgtcaaac gcgacagcgc cgaaacgggt gtgctgttcg
      541 agttaatagc gcatcgctat gaacgtggga gcctggtgat aaccagtaac catccgttca
      601 gcatgtgggg cagcatcttc gtggatgaga ctatggcggt ggcggcagca gaccgactga
      661 tccatcacgg atatatgttc gaactgaaag gggaaagcta caggaaaaag acagcgaagg
      721 cagtaacaag cgtgacttga tgtcgcactg aaggggcggt cagtataatt ggcgccagtc
      781 ggcaaaacta gttgacgtct aatacctgga cggtcacttc tgagaaaagg catttacaca
      841 gaatcttaaa caggctcacg gagttctttc agaaaaaggc ctgccgaatg gcaggcctat
      901 cccattcgtc agaaaacata acggaagttg gcgtttatcg cattatccac attatatttc
      961 ccgaacgctg atttctccag ctccagtccc aaacgcatat tatccttaat ttccgcattc
     1021 atccctaccg tcatcagcat cctgctatct ttttcacctt cgaaacgttt ctctccggaa
     1081 gcatcctgca gaaccgtttc tccgttcgcc agcaggtcga actgataacc cagcccggct
     1141 cgagctgtga ttttccagtc gtctccggag aaggctcttc ccacgtcaac accagtacgg
     1201 ccaatcagtg ggttataatc cttgtctttc atgctcagag ccattccccg gtcttcccag
     1261 ctaaaagctt ttcctgatac agaaccgtaa accagctcta tctgtggctc cacccaggac
     1321 tctttcgtca ggtggtaacg ataaccaact tcagcaccgg cataccagga atgagagctg
     1381 tagtcttttg ttcccagtga ggcaaaattc gccgtgtgct gattatcatg atggagatat
     1441 ttaccaatca ggtcaaaata agctccggaa ttaaagagtg cagaggcata cagaccgcca
     1501 cccacggatt tgttttttcc actgaatgcg tggctgcttg cattgctgtc cgtataggtc
     1561 agcaatgccc ccgtaaataa atccacaccg tccagctcat gctttctgtc gacaccaatc
     1621 tgaacgtgag tgtagttatc gctgtagtca ccatctgccg aaccggtacc attcattatg
     1681 cgtgcccaga caccggcatc cccctgagta tcccgcaggt cacccatacg tttgttcagg
     1741 ttattgacct ctgtaaggaa ggacttataa cctgatgcca taaagtcttt tgcggctttc
     1801 gaggctccgg catcagcggt agtctgatat cctgtcagca cccattttgt ggcatcatcc
     1861 gttttttccg tgctgattac tggcgtaacg ttactgaaac caatctgctg cgtaccggca
     1921 gtgaacgttt tctcatcact ccctttcggt gcggtaatta acgtcacgct catttcactg
     1981 gctggtttgt ttaaaaagtc aaccaggacg ctattattac tgcctgacag tttgtctgtg
     2041 acattcaact ggtcagccca gctgttgttg gtatgcatca ggaacgtggt attatctgca
     2101 gaaagctctt ttacttgtaa agtggcaaag tgtttcccgt cccccagttc aattcgcgag
     2161 cccttagcct tcagtgcacc ggcctctgat tttccgtcag agtaccacag agcattgatc
     2221 atatttaccg tactctgtgc ccccgtaata ctgcctctca gaacagcgtt atatcccttc
     2281 attaaggagc tgaataacgg agagtccgtc ttccctgcat cagcatcact gtctcccaga
     2341 ttaatggttg ccgcagtggt tcctgcatga atatccgaac tcagatagcc catattttta
     2401 acagaaaagc tggcgttatc ttcgaggtta atcccttctg ttgtagaaat tacgggtgag
     2461 taatacccct gtttctgcgc agaaggcatc cccgtcaggg tcagagaagc attttctttt
     2521 acatccacag ccccggtgct gaaaataccg ctctgagccg taagttttgc tccttttccg
     2581 agggtcaggc tgctgttgac aaagctgcca acacggtcaa aaacagcatt ctgagaggtc
     2641 acactcacgg tgctatcata agcctcaata ccaccggtga atctctccat gatttgcagg
     2701 gatgatttat tttccagcgt aacatttccg ctgtaatcac tctggtcacc aactgtggtt
     2761 gcagtggcac tcccttcaac agaggagacc gcattaccgg ttccgtcact cctgtcagta
     2821 aagacacggc tacttcccag aatgactgta cttccattgg tggcgttaat atctccctgc
     2881 atcttgccgt ttctggacag atggaatgtt gccgcatcga gtttcagttt ttccagcgta
     2941 aactgacggg tttcccagtc gctctggctg gaagaagtcg tcgttccagc atgaataact
     3001 ggatgaccct ggaatatcag cgtgccggac ttcttggtta tcgaaccttc tggcaggtta
     3061 actgaaccat caagagccag tacatcctta cctgagagct gcggaatatt cacatccata
     3121 ttgccgttta gttggccgtg atacatataa ggctggctgc tggcctcaat tttttgttgc
     3181 ttcacagtat caatggcttt attacgatct tttccgacat tctgccagac actgctgttg
     3241 ttcagatcgg agaagaacgg ggaatatgaa ctcgctttca ggatgaaata ttgcttggta
     3301 gaactgtcat aatataggtc tcccggcgct cctctccccc caaaaatact gacagtatta
     3361 accggtacat tgatgtcact ggcttttaat gtctgaaggt cgagtgttac cgttgatttt
     3421 ttctcactgt tattggtaat gatggccccg tagtcagccg cctgaagacg ggtaaaggtc
     3481 agattattac cattcagttc cagacgtcct ccacggtatc cccacgaaat attatccgga
     3541 ttaacttgct gtgagtcaga aagcacaact gtcggacgac cgctggcaat accaacagag
     3601 ctgaaagcct gtacctttcc gtcagcatcc gcctgctggt tgagaataac ggtaccgtcc
     3661 cccaccttga gtccgcctgc attcacaccg gtcccgttta ctgtcagggt accttcgcca
     3721 gttttatgca ggttatcacc ggcaactccg ttcacctgcc acagaacgtg ggttccttta
     3781 tcggtgataa tcccgccccc catccatgtc tggccgttca gggcggacac cctgtaatcg
     3841 ccggcaaact gcagataccc agctccctga ttaacggaat tctgcagaac gacctcgcca
     3901 ttattgccgg tgaacaggag gttttttcct gcgttcagat catttccttt ctttccatgc
     3961 atatcccaag tcgtgtttcc ctgagtcagt gtgccggtgc cattagccgc atcatatttc
     4021 cactgcagta ctccttcacc agaggtgtag gctatagttt tgtcaaaatc attttgcggt
     4081 tgctgaccaa ggaaatcctg agtggtgacg acccagttat ttccatagtt agttcctgag
     4141 gaaagcacgc caatcagtac ccattttttt tgcagggaat cataagcaaa caggggggag
     4201 ccgctgtccc ccatattcag ataattagcc agaggtccat tcgcgggatt aaaaatgtca
     4261 ccggtctgag ctgtaatcat ctgtccgttg ttataggagt tcagcgcgct gacggttccc
     4321 ccaacaagaa atccgccatt ggtatacact tgggtgcgat taccattttt gtctttgacg
     4381 tactgcatcc cccccccaag gcgatagaat tcagtaaagc gtccgccggc ctgataggca
     4441 ccgctcacag ccccgatgtc agacacttct gccggcgcca cctctgtcac cagtttactc
     4501 aggcggcggg ttttaatatc aagaccgcta ttattatttg taccaaccgc agtataggtg
     4561 tttttggcgt atccgaagct catcgtgtca gaaccgctga catgtttggc ggtaaccaca
     4621 tactgagggg agtaaagtgt atggtctccc ggaggcaggc taccggtagt aatagtggca
     4681 ctactgaaat cagccatcgg ggctttatca agtttaccga ccagattgcc ctgcttgtcg
     4741 taaatggaaa tattcgtggt ccccggcgta aactgacctt tattctcggc aaagtcgcgg
     4801 aatatctgat aagggatctc tgcactgacc gttgctgaca gagctggcca tgccgaagaa
     4861 agtgataaag ctgcaacaga cgtaagaata acccgacgtg aaagtctgcg atggcttttg
     4921 caggtaaccc tgcgggccag ttcggagaca gcaatcaggc ttttggttat atggcaatac
     4981 ttaagataat atattttgtt catatagaaa catcaaccta attttgacaa agattaatta
     5041 cataggatgt agatggagca gtccgagatt ctactaatat caattattaa gcatctgttt
     5101 tttagttggt gtaatataat ttttattaag gtaagcggaa aaacaaccaa aaaaaacgca
     5161 agatgttatt tttttatcat tttgagccag tttggctggg aggatgctct tctcttaagc
     5221 ttcttgctca actttaaaaa tagttatgcc agttcggtat cgataagggg cagtttggat
     5281 atcg
//
LOCUS       DADLSB010000222         5169 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.222, whole genome shotgun sequence.
ACCESSION   DADLSB010000222 DADLSB010000000
VERSION     DADLSB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.222"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            11..596
                     /locus_tag="JN331_003868"
                     /pseudo
     CDS             11..596
                     /locus_tag="JN331_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            609..1367
                     /gene="chbG"
                     /locus_tag="JN331_003869"
     CDS             609..1367
                     /gene="chbG"
                     /locus_tag="JN331_003869"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY7261856.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(1414..3665)
                     /gene="katE"
                     /locus_tag="JN331_003870"
                     /pseudo
     CDS             complement(1414..3665)
                     /gene="katE"
                     /locus_tag="JN331_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
     gene            3848..4111
                     /gene="cedA"
                     /locus_tag="JN331_003871"
     CDS             3848..4111
                     /gene="cedA"
                     /locus_tag="JN331_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY7261857.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            4388..5169
                     /locus_tag="JN331_003872"
                     /pseudo
     CDS             4388..5169
                     /locus_tag="JN331_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1322 a         1131 c         1309 g         1407 t
ORIGIN      
        1 tctgaatctg ttgccgtgtt cttatttgct ttattacttc aagcaaaaag agatgctggc
       61 tattgaaatg ggcgaatact acaaaggcgg cgcacgagcg caggtagtac agaaagtcga
      121 gaaacaactt tttgagctgt ataaaaaccc ggagttgaaa gttaagccga aagaactgga
      181 acagcgcggt ggggcttatt actctgatgc agcgtgcgaa gtgatcaacg ctatctacaa
      241 cgacaagcaa gctgaacatt acgttaatat cccgcatcat gggcatattg ataatattcc
      301 ggcagactgg gcggtagaaa tgacctgtaa gctggggcgc gacgccacat ccgcgcatta
      361 cgcatttcga tgataaagtg atggggctga ttcacaccat taaaggcttc gagattgctg
      421 ccagcaacgc cgcacttagc ggagaattta acgatgtgtt actggcgcta aaccttagtc
      481 cgttggtgca ttccgatcgc gatgctgagc tgctggcacg cgagatgatt ctggcgcacg
      541 agaaatggct gccaaatttt gccgactgca tcgcagagct taaaaaagca cattaaccga
      601 ggctgattat ggaacgctta ctgattgtta atgccgatga ttttggctta agcaaaggtc
      661 agaactacgg cattatcgag gcctgtcgca atgggattgt cacatcgacg acggcgctgg
      721 tgaatgggca ggctattgac catgcggtgc agttgagtcg tgatgaaccg agtctggcca
      781 tagggatgca ctttgtcctt actatgggca agccactgac agctatgccg gggttaaccc
      841 gcgatggtgt gctgggaaaa tggatctggc agttggcaga agaagatgct ttaccgctgg
      901 aagaaattac tcaggagctt gccagtcagt atttgcgttt cattgagcta tttggacgca
      961 aacctacgca tcttgatagc catcatcatg tgcatatgtt cccgcagatt ttcccgattg
     1021 ttgccaggtt tgcggctgaa gaggggattg cgttgcgcat cgaccgtcag ccgctatcta
     1081 acgctggtga tttaccggct aatctgcgca gttcgcaggg attcagtagc gcgttttatg
     1141 gtgaagagat tagcgaggca ctgttcctgc aagtgctgga tgacgcaagc catcggggtg
     1201 atccttcgct ggaggtgatg tgtcatccgg cgtttatcga taatactatc cgtcagagtg
     1261 cttactgttt cccacggtta acggagctgg aagtgctgac atcagcgtcg ttgaaatatg
     1321 caattgctga gcgtggttat cggttgggta gttatcttga tgtgtaagta aggtggtaag
     1381 gtcactcccc aggggacgtg accttatcca tatttacgcc ggaattttgt caatcttagg
     1441 aatgcgtgac cacacgcggt gttctgccat cagcgttaac agttcatcca taaaactacc
     1501 gtcggcgctg tcagcttcca caatcccttc ttcaccctgg tcagctacct tgattgttgc
     1561 tttaaacttg cgcgcgtctc ccgccagcgc aatcggttta aggtgtttgt aggcttccat
     1621 cagatagtag ttggcatcgc cgttgtcagc gatatccgcg atattgccgc aagggacaat
     1681 gaccgcatcg accgtcagcg aaggtgcacc ggcaaaggta gcggctatag gcaacaccgt
     1741 accgtcatcc gcagtcactt cacccactcg ggagtagagc agtttggcat gaacgccttt
     1801 ggccttcagg gccttgagaa tggccagaag gtctgccgat ctcacttcat cattaagtaa
     1861 aatcgctacc acgcgacctt tcacatcacc gtcaggaatg gcgtacaaac ttaaggatgg
     1921 atcctttttc agaccgttga cgtccggagg tggggtgata ttcagctggt cgtcagtcag
     1981 ttcgataccg agatttttcg ccaccgcctg ggccagagtg agatcaatat gcgccagctg
     2041 gtcaacaacg cgctcacgaa tatacggacg aacgactttg cttaactcaa aactgaaacc
     2101 atcgacaata tggcgctgct cgaatggcgt ctgacttagc cagaacagac gcggatggga
     2161 ataatattcg ccaaacgatg ggctgcgctc gcgaacttta ttgccttcca cgcgctcctg
     2221 gtatgattca aaaccgccgc gtttcggccc cggtggtgtt tcgcgcggcc agttatcgtt
     2281 gatcgagttc ggttcataat tcgccgggtt agtgtcgatc cccatacgat gcatgccgtc
     2341 acgctggaaa ttatggtaag ggcaggtcgg gcggttaatc ggaatctcat ggaaattcgg
     2401 cccaccaaga cgactgattt gtgtatcggt ataggagaac aaacgtccct gcaacagggg
     2461 atcgttggtg aagtccagac caggaacaat atgacctgga tggaatgctg cttgttcgtt
     2521 ttcggcaaag aagttatccg gattgcgatt gagcaccatt ttgccgacac gctgaacggg
     2581 caccagttct tccgggatca gtttggttgg atcgagaaga tcgaagtcga acttgaattc
     2641 atcttcttca ggaatcaact ggaagcccag ttcgtattcc ggaaaatcgc ctgcttcaat
     2701 ggcttcccac aactcgcggc ggtggaagtc cgggtcacgt ccggtgagtc atcccaaacg
     2761 agtgaggctt tacctgccag tggtttccag tggaaacgta caaacgttgc cttcccttcg
     2821 gcattaatca ggcggaaggt gtgaataccg aagccttcca tggtgcggta actgcggggg
     2881 atgccgcgat ccgacatcgc ccacatcacg ttgtgcagag tttcaggttg cagagaaaca
     2941 taatcccaga aagtatcgtg ggcactttgc ccttgtggaa ttgcccagtg cggttctggt
     3001 tttaccgcat gaacaaaatc ggggaattta tgcgcatcct ggataaagaa gattggcgtg
     3061 ttattgccaa cgaggtcaaa aataccctct tcagtataga acttggtggc aaaaccacgg
     3121 atatcacgca cggtatcggc agagccagcg ccaccctgaa cggtagagaa cctaacaaat
     3181 actggggtga ttttgttcgg atctgagagg aaatccgctt tggtgatatc gcttaagctt
     3241 ttatatggct ggaaataacc gtgagctgct gatccgcgtg catgaacaat acgttccggg
     3301 atgcgctcat ggtcaaagtg ggtgattttc tcgcgcagaa taaaatcttc cagcagcgtt
     3361 ggaccacggt taccggcacg cagtgagttt tgatcatcgg cgatgcgcac gccctgatta
     3421 gtggtcagcg cataattttc actgcctttg cgtacgtctt ccagagaatt aagtttttcg
     3481 ttacgtgtat caggggcttt caggctccca ggggcggtag gttgtgcacc tggcggtgtt
     3541 ggttcagccg ctggacgatg agatccgtcc tcaggtgcca gtgagtccat ccccggtttc
     3601 gcttcgctgg aatcgtgtag tggtgactgg tgctgatgtg ggttcttttc gttatgttgc
     3661 gacattgaac tcgtctcctt aatttattac tgaaagggcc gctgttttgg tcaaaaaact
     3721 ctctaactat agaccaccag ccagatcccg cttcggagac tattctggaa acggtgttat
     3781 gaaatacacg caaagcggga cgtacagggg ctaaatcggc taaactacag tttttctaat
     3841 ttttaatatg cgtttagtga agccagtaat gaagaaaccg ctccgccagc aaaaccgcca
     3901 gattattagc tatgtcccac gcacggaacc cgcgccgcca gaacatgcga taaagatgga
     3961 ttcgtttcgt gatgtctgga tgctgcgtgg caaatatgtt gcgtttgtac tgatgggaga
     4021 gtcatttctg cgctcaccgg cgtttactgt gcctgaatct gcccaacgtt gggcaaatca
     4081 gatccgccag gaaggggaag tgactgagta aggattacat acctcccggt tttgccggga
     4141 gttacatgac ctgaaattct gcatttcgtt gctacattca ttcaaaccac ctgtaacaaa
     4201 aagctgcctt tgacgagcta ttgctataaa taagtgatct tttttcttta attgtttatt
     4261 tattttaaaa tggttaagtt ttgctaatca tgatgagcta tttttgatag ttcatcctgt
     4321 ggcgtcgctc ttaaacttat attgtaaatg acttagattt aaataagata ataattttgt
     4381 taacaatatg aggcgggaga tgttttctac aaacttaata cagagtaatt atggtgattt
     4441 aaatattaaa agcctggctt tcgactcttt taaagaaagg cttgagtcta ctatggcggc
     4501 actcactttc tttatatcaa cgggtcaatg tgactgcgat gaaatagcag agtcgaactt
     4561 caatcgtatg attgcctata tgtctaacat taactatgat gcctcaaagc taggagcgcc
     4621 agcacttagt tttgatacgt atctgcagga caatgttaaa tatagagtga taattaataa
     4681 tctttatggc agtgagtttc ggataagaga tattaatgag aattttgttg ggatggattt
     4741 tacctgtgaa ttccgacccg aaaaaatgac gaatttgatc aatataaaaa ataggttggt
     4801 tattcattat ttaaggacta tatactatga aaaaaattat atacatcctg ccgggagcat
     4861 ttttgctgcg attcaaaaaa aatgagagtc tcctaaagtt tccttcgata atatcaatgc
     4921 ttaatatcaa tcttcttttt aatccactaa atcttccggg aatgggaagt ggtgtgcttg
     4981 aagatattat gagtattcca gatagttcac ttagaaaacg tctcgggtat gaggttttaa
     5041 gcttctcact acaggcacat tcattgagcc aggaatgtat tgataagttg gacatttttt
     5101 tcgctgacga tctgtttaaa tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa
     5161 gtaaagcaa
//
LOCUS       DADLSB010000223         5153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.223, whole genome shotgun sequence.
ACCESSION   DADLSB010000223 DADLSB010000000
VERSION     DADLSB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.223"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            135..554
                     /locus_tag="JN331_003873"
     CDS             135..554
                     /locus_tag="JN331_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF342 domain-containing protein"
                     /protein_id="HAY7261858.1"
                     /translation="MAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRREFETS
                     ELIRAYGELKRNETPERHTQGHSEKPHDQQIERILRELNELKQCLTLMLEDKQAQDTE
                     RRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
     gene            635..1054
                     /gene="exc2"
                     /locus_tag="JN331_003874"
     CDS             635..1054
                     /gene="exc2"
                     /locus_tag="JN331_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HAY7261859.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
     gene            complement(1279..1602)
                     /locus_tag="JN331_003875"
     CDS             complement(1279..1602)
                     /locus_tag="JN331_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HAY7261860.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1886..2080
                     /locus_tag="JN331_003876"
     CDS             1886..2080
                     /locus_tag="JN331_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7261861.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
     gene            2268..2639
                     /locus_tag="JN331_003877"
     CDS             2268..2639
                     /locus_tag="JN331_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261862.1"
                     /translation="MSKKQSTGRSQRRRRFSIGSAPLTSITKIDAQARGGETRQDYKD
                     TRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFLIAHAVGISARCRSFAPSWAVCT
                     NPPFSPTAAPYPVTIVLSPTR"
     gene            2567..2725
                     /locus_tag="JN331_003878"
     CDS             2567..2725
                     /locus_tag="JN331_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7261863.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLAPLAAAVGNLVSRAMM
                     AQMVQQSS"
     gene            complement(2922..3059)
                     /locus_tag="JN331_003879"
     CDS             complement(2922..3059)
                     /locus_tag="JN331_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 lysis protein"
                     /protein_id="HAY7261864.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISV
                     Q"
     gene            3107..3448
                     /locus_tag="JN331_003880"
     CDS             3107..3448
                     /locus_tag="JN331_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HAY7261865.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKILYAIV
                     ISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFG
                     LLGLFISIKNN"
     gene            complement(3445..5010)
                     /locus_tag="JN331_003881"
     CDS             complement(3445..5010)
                     /locus_tag="JN331_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="HAY7261866.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSK
                     SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEAL
                     RHNSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIE
                     KEQAETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRL
                     SSSIHARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLR
                     ARAGDEMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKT
                     AQTNLLNSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAF
                     EKYKDVLNKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDI
                     LKIKDTGDWKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFID
                     KNKLNTINEVLGI"
BASE COUNT         1457 a         1292 c         1062 g         1342 t
ORIGIN      
        1 aaaaaaacag ccacccacac cacgtgctat ttccacccga tgccacaaaa accagcacaa
       61 acattaccgt tctcagacct cattatgttt tactgaaacc atgagatgag acacctatgg
      121 gacactgtaa ctttatggca tggcacacac tccgggacgc actgaaaatg actggaagat
      181 cgcgttcaca gttttaccgt gatatgcgct caggtcttgt cagttaccgt accagccagg
      241 acggacgacg ggagtttgaa accagtgaac tgatccgggc atacggcgaa ttaaagcgga
      301 atgagacacc agaaaggcac actcaaggac actcagaaaa acctcatgat cagcagatag
      361 aacgcattct ccgggaactg aatgagctga aacaatgcct gacgctgatg cttgaggata
      421 aacaagccca ggatacggag cgccggcgcc aggaagcaga acggaaacag ctacagaatg
      481 agatagccca gctcaggcag gctctggagc aggaaaagaa acggggattc tggtccaggc
      541 tgttcggtca ctgaacgcgc tcagaaactg atgataagat aatcttcgga taataactca
      601 ccgagaataa atactttaag gtagggagac actcatgaga cgtaccggaa acaaactttg
      661 tcttatcgcc atgataacag caacagtagc tctcacagcc tgtaccccaa agggcagcgt
      721 ggaacaacat acccggcatt acgtatatgc ttctgatgac ggttttgatc ccaacttttc
      781 cacccaaaaa gccgacacaa cacgaatgat ggtgcctttt tttcggcagt tctgggatat
      841 gggagctaaa gataaagcga caggaaaatc acggagtgat gtgcaacaac gcattcaaca
      901 gtttcacagc caggaatttt taaactcact ccggggcaca actcaatttg cgggtactga
      961 ttaccgcagc aaagacctta ccccgaaaaa atccaggctg ctggctgaca cgatttctgc
     1021 ggtttatctc gatggctacg agggcagaca gtaagtggat ttaccataat cccttaattg
     1081 tacgcaccac tgaaacgcgc tgaacgcgat tctgacgcag acaccgcaaa aagagatgtt
     1141 ttaccccgga aaaacaggaa ggatgcgtca ggatcgtttt cagctctctg catagctatg
     1201 catgaaagcg aatggtgatc actttgggag cttacggtgt tcataccgtc agttttcgac
     1261 agttttctct ccgggaagct aatctgccat aagcctggat aacagggcac ggtgatactc
     1321 cgtaatagcg atcagcacct ccgcatactc cctgtccccg acacgcttgc cgtcgataaa
     1381 gagttttttc tgtaacgccc ccagtctcat aacttctttc agtacccgat catcagtcag
     1441 tgagttaact ttcttaccga gagctgccgc ccgtaaaaaa ccggaaacgg tcttgccgca
     1501 ttcagcagcc ctttttctga tttcctggtc ttcttctgcc gtcagacgaa cagggcgact
     1561 gatagcccgc ctgcgcgtat tacttccact tcgttttgtc ataaaacatg gctccgtatc
     1621 tgacatgggt gtcggggcaa agccctgacc agggtaattg taatagcgtg catgtatgcg
     1681 cggtataaca attacacatc ctgtcctgtc agcaagttcg aattaccgac aaaggcacgg
     1741 cagttttttg gcagtgacag tattgtcagg cagaaatacc cgtcaaagcg tcaaacgaca
     1801 gaataccggc ggtaaaacag tcgaaaagtg gtacgcgata taatcacaca acgcagatga
     1861 cagacagaac ggagaaaacg aaaagatgaa caagcagcag caagccgtac tcaacatggc
     1921 aggattcata aaaagccaaa gcctgacgct gctcgaaaag ctggacgcac tcgatgctga
     1981 cgaacaggct accatgtgtg agaagctgca cgaactcgca gaagaactcc agaacagcat
     2041 ccagacacgc tttgaagtag aaaatagcac agagagataa cgctcaggac aggatgccga
     2101 acggcacaac agagcgtaag gagaaaatgt cacataaggc gctcttccgc ttcctcgctc
     2161 actgactcgc tacgctcgga cgttcgactg cggcgagcgg tgtcagctca ctcaaaagcg
     2221 gtaatacggt tatccacaga atcaggggat aaagccggaa agaacatgtg agcaaaaagc
     2281 aaagcaccgg aagaagccag cgccgcaggc gtttttccat aggctccgcc cccctgacga
     2341 gcatcacaaa aatcgacgct caagccagag gtggcgaaac ccgacaggac tataaagata
     2401 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2461 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2521 ttggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2581 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2641 acacgactta gcgccactgg cagcagccgt tggtaacttg gttagcagag cgatgatggc
     2701 gcaaatggtg caacagagtt cttgaagtag tggcccgact acggctacac tggaaggaca
     2761 gtatttggta tctgcgctct gctgaagcca gttacctcgg ttaagcagtt ccccaactga
     2821 cttaaccttc gatcaaaccg cctccccagg cggttttttc gtttacaggc agcagattac
     2881 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac agagatcccc
     2941 gtcaatttag aagaagatga tggagccaca gtccctccct gaacatcacg tatatagtta
     3001 gcctgacatc caacaaggag gtttatagcg aatattccca caaaaaatct tttcctcata
     3061 actcgatcct tataaaatga aaagaatata taacgaggtt taatttatga gtttaagata
     3121 ctacataaaa aatattttgt ttggcctata ctgcgcactt atatatatat accttataac
     3181 aaaaaacaac gaagggtatt atttcctagc gtcagataag attctatacg caatagtgat
     3241 aagcactatt ctatgcccat attcaaaata tgctattgaa cacatatttt ttaagttcat
     3301 aaagaaagat tttttcagaa aaagaaaaaa cctaaataat gcccccgtgg caaaattaaa
     3361 cctatttatg ctatacaatc tactttgttt ggtcctagca atcccatttg gattgctagg
     3421 actgtttata tcaataaaga ataattaaat acccaacacc tcatttatag tattaagttt
     3481 attcttatca ataaaggcac atagaatgcc tgtaacaata gcaatccccc agatacctaa
     3541 tgtagttcca gcaagcacac taaaaagtaa aacaacaaca taactaactc cagcatctac
     3601 agctttcttc tctaatgtaa gaaatagcgg cttccagtca cctgtatcct taatttttag
     3661 gatatcagat accacatcat atccaaaaga aacatgtccc gtaatcttca agtacttggc
     3721 aaactgatct aaatgcttag cccagtcttc atacttaacc gcctccagtg cattgaaaat
     3781 tgcatcacgg tctgctttgc tgaatttctt atttaataca tccttgtatt tctcaaaagc
     3841 agctagagct tcattcacat tgcttatttt cttaccttta gatttatcag caagttcctg
     3901 tgccattttt gaatattttt caccatattt ttcagatagc gtttgataaa agctaactgt
     3961 tgcatccaca gcatccttaa tctgcgagtt caggagatta gtctgtgctg ttttcaaatt
     4021 atcttcagca tcatgaatac gtgaaactgc tgtacttcgc ttattattag cctgagaaat
     4081 agcctcttgg attccattta tctcagagct aatctggtta agacgcgttt ctgatgctgt
     4141 tacctgcttt tgtttttcct ccatctcatc accagctctc gcccgtagtc tggtggcctc
     4201 aaaaaaagga cgactctgaa gcggatcatt cgccctagga gataaaagtt taaccctttc
     4261 atccagttct ttatatttag cagatgcctg atccaactca tttcgttttc cggacagcgt
     4321 attcgtttct gcatcacggg catgaatact ggagcttaaa cgggtattga gcgtattaat
     4381 ctcttcatcc actttcgcca gctcagattg tgcagcagca agattttttt gtgccacctc
     4441 cacagcccgg gcctcttcac tcaatgctgc caggcgtttc tcttcatcct cagccagttt
     4501 caattggcgt tctgtttcag cctgctcctt ctctatctct ttacgtcgtt gttctgcttc
     4561 ctgaaaagcc ttttccgcag cttccgcttc tttacgggct ttttcttctg cttttgcaag
     4621 gcgcaaccgc tctgcttctg cctgcatcgc tgcattattg gcatgagcca ggtcaataac
     4681 ctccggatga gtggaattat ggcgaagcgc ctcattcaca atatccttca gatgttgagt
     4741 cagcgcatcc cggtttgctt ttgctttagc ctgtgcttct gcggcagctt ttgctcgggc
     4801 agcctgttct gcctgcgttt tcttcaattg agcagtagac catttagctg tggcatgaat
     4861 ggctgcagaa ctttcacttt tgctacctcc agttccacca ccaccgccag agccactccc
     4921 gtctggatta ccattcaaaa gagtaatgat tacctgcccc ttatcatcat aaggaacacc
     4981 atctttatag tacgctacag ctgtttccat tataaaatcc tctttgacat taaaaacaaa
     5041 tagttaaaag aaatatactg tatatataat cactggcttt atatacagtt taaaattacg
     5101 ccgctgcatt ttccctgtca accctgtgga ttttcatttt tgtgaaaacg atc
//
LOCUS       DADLSB010000224         5085 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.224, whole genome shotgun sequence.
ACCESSION   DADLSB010000224 DADLSB010000000
VERSION     DADLSB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.224"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1201)
                     /gene="ascF"
                     /locus_tag="JN331_003882"
     CDS             complement(<1..1201)
                     /gene="ascF"
                     /locus_tag="JN331_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7261867.1"
                     /translation="MAKNYAALARSVIAALGGVNNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISCFICG"
     gene            1458..2471
                     /locus_tag="JN331_003883"
     CDS             1458..2471
                     /locus_tag="JN331_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7261868.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            2620..3147
                     /gene="hydN"
                     /locus_tag="JN331_003884"
     CDS             2620..3147
                     /gene="hydN"
                     /locus_tag="JN331_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY7261869.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            3300..>5085
                     /gene="hypF"
                     /locus_tag="JN331_003885"
     CDS             3300..>5085
                     /gene="hypF"
                     /locus_tag="JN331_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY7261870.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCG"
BASE COUNT         1181 a         1295 c         1445 g         1164 t
ORIGIN      
        1 cgccgcaaat aaaacagctg ataagactgg cgataagcgg acgtttcagg cggatcgcca
       61 cgccgtataa cgccggttcg gaaatccccg ccataatggc tgatgccgcc gcagccagcg
      121 ccgtctggcg cagttccggg tttttcgttt tccacggcac cgccagtgat gaaccgccca
      181 gcgacaggtt agcgccgatc tctgatggca tgaccattcc ttctttgccg gtttcggcaa
      241 tggtctgaat gatggttggc gtaaagacgc ggtgcatccc ggtcattacc agcagaggcc
      301 acagcgcgcc cataatggcg actgaaagcc agcccagata accatgaatg gtgtaaacca
      361 gcgccgaaat ggcgctaccg atccagatac caatcgggcc aatcagcagg attgccagcg
      421 gtgcggcaat caacacaatc agcattggct tgaggaagtt tttggtcacc gccggtgtaa
      481 tgcgatccac ccagcgttcg atatatgaca ggcaccaggt catcaccagc gccgggatca
      541 ccgtgtaggt gtatttcacc gcggtgaccg ggatcagggc aaattcgaca tgttcaccct
      601 gggccgcttt cgccattaat tcaataaaac tcggatgtac cagcacaccc gcgatggcaa
      661 tcgccagcga catattggtt ttaaatttga tggcggcaga tgccgcgacc attagcggca
      721 ggaagaagaa agcaccgtca ccaatcacat tcagaatggt taaggtcggc gatccttttg
      781 tcagcacgcc gctcatctcg aggatcatcg ccagcagttt gaccatcgat ccgccgataa
      841 tcgccgggat cagcggtgac atggtgccga tcagcgcatc gaggatcccc gcaccaatgc
      901 gacgcagcgt gagtttgggt ttacccacgg gctgtgcggg ctgcatatct cccggcagca
      961 ggctgacgac ttcctgaaag gcctgcgaaa ccgtattgcc gataattacc tgacactggt
     1021 tgtcactacg taccacgccg agcacgccgg ggatggtttt taacgtcggg ctgtcgataa
     1081 gctggtcatc tttgataaca aagcgcaaac gcgtcataca gtgcgtgact gccgagatgt
     1141 tattaacgcc gcccagtgcc gctatcaccg agcgtgccag cgccgcataa tttttggcca
     1201 tcggatttta tcctgttatc agtagggtag atgtagttgt tatcagtcga cgtgacggaa
     1261 aaatgcctat gctgaaacgg aattaagaaa ccggtttcac aaaatcatga atagttgtca
     1321 ctaaataagc aagagagtta attgttcatt ttttatattt gtgaaaccgg tcacctgaat
     1381 atttttcccg ccgattgcgc aaggacaatc ggcaaacggt gcagtacact gcgttgattg
     1441 cgtgtaaagg gatgaaaatg atgacgacga tgctggaagt ggcgaagcgc gccggggttt
     1501 caaaagcgac cgtttcccgc gtgctttcag gtaatggcta cgtcagccag gagactaaag
     1561 atcgcgtgtt tcaggcggta gaagagagca gttaccgccc aaatttgctg gcccgcaatc
     1621 tgtcggcgaa gagtactcag acgctggggc tggtagtgac caacacgctt tatcacggca
     1681 tttattttag tgaattactg tttcatgccg cgcgaatggc ggaagagaaa gggcgacagt
     1741 tgctattggc agatggtaaa cacagcgccg aagaagagcg ccaggcgatt cagtatctgc
     1801 tggatttgcg ctgcgacgcc attatgattt acccgcgttt tttaagcgtg gatgagattg
     1861 atgacatcat tgacgcgcac agtcagccga taatggtgct taatcgccgc ctgcgcaata
     1921 atagcagcca tagcgtctgg tgcgatcata aacagaccag ctttaacgcc gtggcagagt
     1981 tgataaacgc cgggcatcag gagattgctt tccttaccgg ctcgatggat tcccccacca
     2041 gcattgaacg tcttgccggg tataaagacg cgctgtcgca gcatggtatt gcgctcaatg
     2101 aaaaacttat cgctaacggt aaatggacgc ccgccagcgg ggccgaaggg gtagaaacgt
     2161 tgctcgaacg tggggctaaa tttagcgcgt tagttgccag taacgacgat atggcgatag
     2221 gtgcgatcaa agcgttgcac gagcgcggcg tagcggtgcc agagcaggtg tcagttatcg
     2281 gattcgatga tatcgcaatt gccccctata tcgttccggc gctctccagc gtaaaaattc
     2341 cggtgactga gatgattcag gaaattattg gacggctgat ttttatgctc gatggcgggg
     2401 atttctcacc gccgaaaacc tttagcggaa aactgatccg ccgcgactca ctcattgccc
     2461 tttcgcgata aacattgtca tcacatgtca tcgtcactag tgtcgatgac atcttttttc
     2521 ctgcattaca gaaattaatt tccttaaata acagcaaatt aaaaactggc atgatttgtg
     2581 aatgtatcgg cgcattaact gtcattgctg gagaatttga tgaaccgttt catcattgct
     2641 gacgcgagta aatgtattgg ttgccgtacc tgtgaagtgg cgtgcgtggt ttctcatcag
     2701 gaaaatcagg actgtgcatc gctgaccccg gaaacatttt taccgcgtat ccatgtcatt
     2761 aaaggtgtga acatttccac ggcgacagtt tgccgtcagt gtgaagatgc accgtgcgct
     2821 aacgtctgcc cgaatggtgc tatcagccgt gataaagggt ttgttcatgt gatgcaggaa
     2881 cgttgcattg gttgcaaaac ctgcgtggtg gcttgcccgt atggcgcgat ggaagtggtg
     2941 gtacgtcctg tgattcgcaa cagcggcgcg ggtctgaatg tacgggctga caaagccgaa
     3001 gccaataaat gcgacctgtg caaccatcgt gaagacggcc cggcgtgtat ggcggcttgc
     3061 ccgacccatg cgctgatttg tgtcgatcgt aataaacttg aacaactgag cgcagaaaaa
     3121 cgccgccgta cggcgctgat gttctaaaaa tcttacagaa ctgttctggc atcaaaggaa
     3181 ttcccggaaa tattcgtaga atacggagcg ggcggtatcg ccgacctgag actgccggat
     3241 acgacgctgt cgtgtccggc agggcgggtt acgcaattca tgatggcggg aataactcaa
     3301 tggcaaaaaa cacatcttgc ggtgtccaac tgcgtattcg tggcaaagtg cagggcgtcg
     3361 gttttcgtcc gtttgtctgg cagctggcac agcaattaaa tcttcacggc gatgtatgta
     3421 atgacggcga tggcgtagaa gtccggctgc tggaagaccc ggaaacgttt cttgttcaat
     3481 tgcatcagca ctgtccgcca ctggcgcgta ttgatagcgt cgagcgtgag ccgtttatct
     3541 ggtcacaact gcccaccgag ttcaccatcc gccagagcac cggcggcacc atgaatacgc
     3601 aaattgtccc ggatgccgcc acttgccctg cttgccttgc cgaaatgaat accccaggcg
     3661 agcggcgtta tcgttatccg tttatcaact gtacccactg cggcccgcgt ttcaccatta
     3721 ttcgcgcgat gccttacgac cgcccgttta ccgtgatggc ggcgtttccg ctatgtccgg
     3781 cctgtgacaa agagtaccgc gacccgctcg atcgtcgctt ccacgcccag ccggtggcct
     3841 gcccggagtg tggcccgcat cttgaatggg taagtcatgg tgaacatgca gaacaagagg
     3901 cggcattaca ggcagctatc gcacagttaa aaatgggcaa cattgtcgcc atcaaaggga
     3961 ttggcggatt tcatcttgcc tgcgatgcac gtaacagtaa cgcggtggcg acactgcggg
     4021 agcgcaaaca tcgcccggcg aaaccgctgg cagtcatgtt gccagtggca gaaagtttac
     4081 cagacgctgc gcgccagttg cttaccacgc ccgctgcgcc gattgtgctg gtggataaaa
     4141 aatacgtacc tgagctttgt gatgatatcg cccctggcct taacgaagtc ggggtgatgt
     4201 tgcctgcgaa cccgctccag catttgctgt tacaggaact gcaatgcccg ctggtgatga
     4261 cctccggcaa cctgagcggt aaaccaccag ctatcagcaa cgaacaggcg ctggcggatt
     4321 tgcagggcat tgccgacgga ttcttgctac ataaccgcga catcgtgcag cggatggatg
     4381 attcagtggt gcgcgaaagc ggcgaaatgc tgcgccgttc gcgggggtat gtgccggatg
     4441 cgctggtttt gcctccgggc tttaaaaatg ttccgcctgt gctgtgtctt ggcgcggatc
     4501 tgaaaaatac cttctgcctg gtgcgcggtg aacaagcggt gttgagtcag catctgggcg
     4561 atttaagtga cgatggcatc cagatgcagt ggcgcgaagc gttacgcctg atgcaaaaca
     4621 tctacgattt cactccgcaa tacattgtgc atgatgtgca tccgggctat gtctccagcc
     4681 agtgggcgag cgaaatgaat ctgccgacgc aaacggtact gcatcatcat gcccatgcag
     4741 cggcgtgtct ggcagagcat cagtggccgc tggatggcgg tgatgtcatt gctttgacgc
     4801 tcgacggtat cggtatgggg gagaatggcg ctttgtgggg cggcgagtgc ctgcgggtga
     4861 actatcgcga atgtgagcac ctgggcggct tgcctgcagt ggcgcttccg ggtggcgatt
     4921 tggcggcgaa gcagccgtgg cgaaacctgc tggcgcagtg cctgcgcttt gtgccggagt
     4981 ggcagaatta ccctgaaacg gcaagtgtgc aacagcaaaa ctggagcgta ctggcgcggg
     5041 ccattgagcg tggaattaac gcgccgctgg catcatcgtg tgggc
//
LOCUS       DADLSB010000225         5067 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.225, whole genome shotgun sequence.
ACCESSION   DADLSB010000225 DADLSB010000000
VERSION     DADLSB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.225"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(108..183)
                     /locus_tag="JN331_003886"
     tRNA            complement(108..183)
                     /locus_tag="JN331_003886"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(148..150),aa:Thr,seq:tgt)
     gene            complement(189..263)
                     /locus_tag="JN331_003887"
     tRNA            complement(189..263)
                     /locus_tag="JN331_003887"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Asn,seq:gtt)
     gene            complement(524..1147)
                     /locus_tag="JN331_003888"
     CDS             complement(524..1147)
                     /locus_tag="JN331_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7261871.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(1144..1332)
                     /locus_tag="JN331_003889"
     CDS             complement(1144..1332)
                     /locus_tag="JN331_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY7261872.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(1329..1691)
                     /locus_tag="JN331_003890"
     CDS             complement(1329..1691)
                     /locus_tag="JN331_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7261873.1"
                     /translation="MNEYQFVLPYPPSLNTYWRRRGSRYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKARLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(1688..1978)
                     /locus_tag="JN331_003891"
     CDS             complement(1688..1978)
                     /locus_tag="JN331_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7261874.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKP"
     gene            complement(1978..2748)
                     /locus_tag="JN331_003892"
     CDS             complement(1978..2748)
                     /locus_tag="JN331_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY7261875.1"
                     /translation="MNAVIIANAEIVPIEFNGERVVTYQMIADIHGVPVATIQNGYAR
                     NSEYFIDREDTYFIDFHENTFRRGFEIPPRGMRVFTESGYLMLVKTLRDETAWRVQRE
                     LVRNYFRGQKEDKSALPDFTNPAEAARAWANEYEQKVKLEQQLAIAAPKVEFADRVGE
                     ASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVN
                     TNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(2763..2999)
                     /locus_tag="JN331_003893"
                     /pseudo
     CDS             complement(2763..2999)
                     /locus_tag="JN331_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3074..3754)
                     /locus_tag="JN331_003894"
     CDS             complement(3074..3754)
                     /locus_tag="JN331_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY7261876.1"
                     /translation="MATQISVETLSPITHNQIPVITTELLAQLYCTEINNIKVNYTRN
                     SERFVEGKHFFKVVGDELKNLRVTLSNSQTPVSPKTRSLILWTERGAARHAKMLETDQ
                     AWDVFEKLEDCYFSQTSKSAGHHEKKTSELSAKEANSLVWLWDYAHRSQALFRELYPA
                     LRQIQSNYSGRCHDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSAWVRLKNKE
                     LPPSLHHY"
     gene            4076..4405
                     /locus_tag="JN331_003895"
     CDS             4076..4405
                     /locus_tag="JN331_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7261877.1"
                     /translation="MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4395..4703
                     /locus_tag="JN331_003896"
     CDS             4395..4703
                     /locus_tag="JN331_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7261878.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4745..4921)
                     /locus_tag="JN331_003897"
     CDS             complement(4745..4921)
                     /locus_tag="JN331_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY7261879.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKPKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4918..>5067)
                     /locus_tag="JN331_003898"
     CDS             complement(4918..>5067)
                     /locus_tag="JN331_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY7261880.1"
                     /translation="DVLPGLEGECVNIRESSAQMSVKRMASLIEYTTAWAIGQGVRFT
                     DRRYE"
BASE COUNT         1216 a         1266 c         1197 g         1388 t
ORIGIN      
        1 tccgcaaata acggatggcg tagttcttat attgggaagg ggaaaagaag gccgcagcgt
       61 aactgtcact gatgaattca ggatagccag tggctacggc tcagttatgg tgctggttaa
      121 cggacttgaa ccgctaccca ttcgcttaca aggcgactgc tctaccatta gagctaaacc
      181 agcatatttg gcgggacagc gtggactcga accacgataa gaaggttaac agccttccgt
      241 aatgaccttt atacgactga cccaaataaa aaatcccgaa agcaggctct attcctatgt
      301 gctcccgagt ttatgatgcg catgtcagtg cgcttagcga aaaacgccgt aaaccctcgc
      361 ccgacagggc ggggagcagt cacaacttaa agatgaggag ggtaaaaaac cgaaagaatg
      421 tgaacggata taaacctgcc attcttgagt caaatttacc caactttatt caaaaagtca
      481 atatcatgcc gttaatatgt tgccatccgt ggcaatcatg ctgctaacgt gtgaccgcat
      541 tcaaaatgtt gtctgcgatt gactcttctt tgtggcattg caccaccagg gcgtcataca
      601 gcggcttaac agtgcgtgac caggtgggtt gagtaaggtt tgggattagc atcgttacag
      661 cgcgatatgc tgcgcttgct ggcatccttg aataaccgac gcctttacat cttccgcact
      721 ctttctcaac aactctcccc cactgctctg tttttgctat atcaactgcc cggcctgtac
      781 cgtggcaatc tctgcatctt gcgcccggcg tcgcggcact acgacaataa tccgcataag
      841 cgaatgttgc gagcacttgc agtacctttg ccttagtgtt tccttcgagc tttgccacac
      901 cacggtattt ccccgatacc ttgtgtgcaa attgcatcag atagttgata gccttttgtt
      961 tgtcgttctg gctgagttca tgcttaccgc aaaatgcagc cattccgaat ccggcttgtg
     1021 attgcgccat ccccatagca gccatcacat cagtaccgga aagagagtca gaagccgtag
     1081 cccgtggtga gtcgctcatc atcgggcttt ttggcgaatg aaatttagct acgctttcga
     1141 gtctcatcgt cttcccctct tgccctgttt gaccatcagg acgccgttaa ctattacgtg
     1201 acgctcgcct ttgctgtctc ggttgtactt gagcactgtt cctcttgcgc aggaaagcat
     1261 cctcgccact tcggtctgat tgcctcgtgt ctggataaga agctctggta tcgtttgaat
     1321 tgtggcgttc atacgttctc cagttcggtg atttttattc caagccttcc gcctggtact
     1381 ttcacaccac gaattacgcg aatgtcatcg aattgctcgt cgtcttccgc aaatccggcg
     1441 tggataagag agtcgagtag ccccttcagg atgttatcga ggtcgcggcg gcgggagtct
     1501 ggaacgtctg cgatgacttt gatgcggagt cgtgctttgg tgaaaatatc taacttgagt
     1561 tggcggatga tttgctgaac gtcttttcgg tatttctggc ctttatcgct gatgtagtat
     1621 cggcttcccc gtcttcgcca gtaggtgttc agcgacggcg ggtatggaag cacaaactga
     1681 tattcgttca tggcttaatc ttcccctcct tcagcagtat cgcctgcgtc ctgatcacgc
     1741 cttcgaggtg gtaaagcctg gcgtctttgt tgtcgagatt atgggtgcgt cggtcgattt
     1801 catcgtgaca cgcgctacaa gcccatgcgc cgatcaggtc gtcaggcttc attcccgttc
     1861 cgcaaattcc agccatccgg taatgtgcca gaactgtagt ttcagggttg ccattgcata
     1921 cgccgtaaat acgtacctgg cattctctgc cgcgtgcttc tttgcgtagg ttagccatta
     1981 agcagcctcc ccggttactt tcagcattcc gttatcgagc agctttctgg tcagccactg
     2041 ttgaccacgc ccggtgattt ttgtggtgaa cgatatctgt attccgtgat ttgtgttgac
     2101 cgctgtttct ttcactgtga aatagccgcg atccatatat tcctgcattg gcacattgcg
     2161 ccgggaacct gaagcaataa ggattttgtg atcacgcatc cacgcaaaca gtttgtttgg
     2221 gccaatacca acaacctttg caaagtttcc aatcaaaatt ccgctggcct cgccaacgcg
     2281 atcggcaaac tcaactttag gtgcggcaat cgcgagttgt tgctccagct tcactttctg
     2341 ctcatattcg tttgcccacg ctctggcggc ttcagcaggg tttgtgaagt cgggcaaggc
     2401 tgacttatct tctttttggc cacgaaaata attgcgaaca agctcacgtt gcactctcca
     2461 cgccgtctcg tcgcgcaggg ttttaaccag catcaaatag cctgattcag tgaaaactcg
     2521 catgccacga ggaggaattt caaaacccct tctaaaggta ttttcatgaa aatcaataaa
     2581 atatgtatct tccctgtcta tgaaatactc agagtttcgc gcatagccat tttgaatagt
     2641 ggctactgga accccgtgaa tatcagcaat catttgatac gtaacaacac gctcgccatt
     2701 aaactcaatc ggcacgattt ctgcgttggc aataattacg gcattcatgc aacacctcct
     2761 tcaacgacaa taatgctgtc gcgcttacct tgttcgcctt cgaagacgta atgctcgtac
     2821 tgaacattga accctaagtt attgattctt tcggaaacct caaattgagg atgccggata
     2881 acaccatttt tagccagcgt ttcgatggta cgtttcacat tgtcatgacg cttacccacc
     2941 aactcagcga tttcaatgct ggtcattttg atggcgttgc tatttatcag ctcgttcatt
     3001 gtcatgtcct ctcatattga aaattcagca ataaaaaacc cagccgaagc tgggtcgttg
     3061 cgttggcaat ctgtcagtag tgatgtagtg aaggaggtaa ttctttgttc ttaagtctta
     3121 cccatgcaga aagattcgtt ggtccgtctg gctcattaat atcaacatct cgtgtgtgat
     3181 tgattaaaac gtctctcgcc attccgataa catacgagaa ctcatgaccg tagtcgtggc
     3241 atctgccgga atagttagac tgaatttgtc ttagcgccgg atacagttcg cggaataatg
     3301 cctgtgagcg gtgggcataa tcccacaacc atacaaggct gtttgcttct tttgcagaaa
     3361 gctcgctggt tttcttctcg tgatgacctg cgcttttcga agtctggctg aaatagcagt
     3421 cttccagttt ttcgaacacg tcccatgcct gatcggtttc gagcattttt gcgtggcggg
     3481 ctgctccgcg ttctgtccag aggatgaggg agcgggtttt gggggaaaca ggcgtttgtg
     3541 agttacttaa agtaacccgc aaatttttca actcatcacc aacaaccttg aaaaagtgtt
     3601 taccctcaac aaaacgctcg gaattacggg tgtaatttac tttgatgttg ttgatctcgg
     3661 tacagtaaag ctgtgccagt aactcggtag tgataacggg aatttggttg tgtgtaattg
     3721 gggaaagagt ttcgacagaa atctgagtgg ccataatgac gccctccggt gattgttttg
     3781 tttatcacca ccgccgacgc caatcggatt gggtggtgag acgtacaggg ttggcgtaac
     3841 cggatcaccg accggcgagc ctttcggctc ccccatacgc cccaccataa ttcagatgcg
     3901 cgtatacaaa cgacaataaa aaacacgctc gcggcgtgtc tctgtcgcgg tgaaattccg
     3961 ggacgccaat cccgacgcca gattttgctg gcgtatgagg aatatagccc cggatatgtg
     4021 ttgtcgtcaa ctacaggcct tgaatgcata taacgatttt gttatgttga tacctatgaa
     4081 ctacactatc gaatactaca gtgaagatgt aaggctggaa gtcgatcggc ttccattgag
     4141 tatgcgtgcc cgataccaac atctcgttga acgtatgaag atatatggca gcaatctcgg
     4201 agaacctcac accagcgcct ttggtgacgg gcttttcgaa ctcagaatta aaggcaacga
     4261 tgggatcgcg cgtgtttttt actgcactct gacagggaaa cgcatcatca tgctgcatag
     4321 ttttgtaaag aaaacgcaga aaacaccgcc agccgaacgc aagaaagctg aaaccagaat
     4381 gaaggaggtt aagcatgact ggtaaacgca ctctccccac catgacacac gacgaaatgg
     4441 cagccaaatg gatggaagac ccggctttta aagcagaata cgacgctatc gctgacgaat
     4501 tcgcactgct tgatgaaatg ctggcagcac gcaaagaagc tggcttaact caggctgaag
     4561 ttgccgagcg aatgggaaca aaagcgaccg cgatcaccag aatggaaagt aatctcgcat
     4621 caggtatcag cggcccatca tttgccacac taaaaaaatt cgcccgcgct actggaaaaa
     4681 aactccagat ccgcttcgtt taaccacacc gcgccgtcat tctggcggcg caccggataa
     4741 atagtcattt cctcgcacga tgtcttagcc accggatatc ccacaggtga gccgtgtagt
     4801 tgaaggtttt tacgtcagat tcttttggga ttggcttggg tttatttctg gagcgtttcg
     4861 ttggaaggta tttgcagttt tcgcagatga tgtcggtgat acttcgtcgc tgtcgtctca
     4921 ttcgtacctc ctgtcggtaa atctgacacc ctgaccaata gcccatgctg ttgtgtactc
     4981 gatcagactt gccatgcgct tcacgctcat ctgcgcgctg ctttcgcgaa tgttgacgca
     5041 ttctccttca aggccgggca aaacatc
//
LOCUS       DADLSB010000226         5036 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.226, whole genome shotgun sequence.
ACCESSION   DADLSB010000226 DADLSB010000000
VERSION     DADLSB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.226"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..207)
                     /locus_tag="JN331_003899"
     CDS             complement(<1..207)
                     /locus_tag="JN331_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HAY7261881.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKP"
     gene            complement(316..729)
                     /gene="hiuH"
                     /locus_tag="JN331_003900"
     CDS             complement(316..729)
                     /gene="hiuH"
                     /locus_tag="JN331_003900"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7261882.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            862..1533
                     /gene="hprR"
                     /locus_tag="JN331_003901"
     CDS             862..1533
                     /gene="hprR"
                     /locus_tag="JN331_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY7261883.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1533..2891
                     /locus_tag="JN331_003902"
     CDS             1533..2891
                     /locus_tag="JN331_003902"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY7261884.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2999..3850)
                     /gene="hchA"
                     /locus_tag="JN331_003903"
     CDS             complement(2999..3850)
                     /gene="hchA"
                     /locus_tag="JN331_003903"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY7261885.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(4444..4653)
                     /locus_tag="JN331_003904"
     CDS             complement(4444..4653)
                     /locus_tag="JN331_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261886.1"
                     /translation="MSGDSGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINLL
                     DKSDYVFAHGLNTDDQLAVGIVYQF"
     gene            4717..>5036
                     /locus_tag="JN331_003905"
     CDS             4717..>5036
                     /locus_tag="JN331_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7261887.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELADAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRA"
BASE COUNT         1419 a          996 c         1190 g         1431 t
ORIGIN      
        1 aggcttgctg aactccagcg gttttccggc gggggcgggc ggacgatcgt tccctttaaa
       61 ccagctaagc agatcggcat gcgcagcgtg aggcaaagaa aatgcagctg cgctgatgcc
      121 cagtgctttt aacacctgcc gacgcttcat aaagaatacc gactcggccg taacatctga
      181 ttcttttaaa aattgattct ttttcataag ccatcacact tttttgtcgt taaccaacaa
      241 gagctgttta ctgttaatct gtcagggagt cacctcccta acagtagcag agatcagctt
      301 ttacatattt acgcattaac tgccacgata ggttgaatac ccatattggc tgagtaacaa
      361 aggcacatga taatgctcgt tcactttatt aatatgaaat tcaacgggaa tctcagggaa
      421 gaaactttca agattttgtt tcttgaaata gtccccggtt ttaaatacga cacggtaatc
      481 gccagtagtc gcagtttgct cgggccacag tgccttaatt cgtccatcct tatctgtttt
      541 ggcggtatta agttgtaacc agccgttatc cgctttcttt tcaagagtga ctgtcacgtc
      601 ggcagcgggt tttcctgttt gctggttcaa aatgtgcacg ctaagaatgt tttgttgtgc
      661 ggcataaacc aaagaaggta atgaaaatgc tgccgttgct accgagagta ctaaataacg
      721 ctttaacatg tttatatcct tgtcatgtga atgagttttg aggatagcga aaaaacgtcc
      781 agggtaacct tacagcagca ttacaatttt gtaatgaacc aggttgtttc tataacatat
      841 gatttatggc atattatttt catgaagatt ctacttattg aagataatca aaggacccag
      901 gaatgggtaa cgcaggggct ttccgaagcg ggttatgtca ttgatgccgt ttctgatggc
      961 agagatgggc tttatcttgc gctgaaggat gattatgcat tgatcattct ggatattatg
     1021 cttccgggta tggatggctg gcagatctta caaacgttaa gaacggcaaa gcaaacccct
     1081 gttatttgcc ttactgcaag ggattctgtc gatgacagag tcagagggtt ggacagtggg
     1141 gcaaatgatt atctggtaaa acctttttca ttttctgagt tgctagcaag ggttcgggca
     1201 caattaaggc aacatcacgc tctgaattca acattagaaa tcagtggctt aaaaatggac
     1261 tctgtcagtc aaagtgtgag cagggacaat atcagtatta cactgacgcg caaggagttt
     1321 cagttacttt ggctactggc ctccagagct ggcgagatta tacccagaac ggttattgcg
     1381 agtgaaattt ggggaatcaa ctttgatagt gataccaata cggtggatgt cgccattcgc
     1441 aggctccgcg caaaagttga tgatcctttt cctgaaaagc taatcgccac aatccggggg
     1501 atgggctatt cattcgtagc ggtaaaaaaa taatgaaaag attatctata accgtccgtt
     1561 taaccttgct ttttatattt ctgcagtctg ttgctggcgc tggaattgta tggactctct
     1621 ataatggcct ggcaagtgag ttgaaatggc gcgatgatac aacactcatt aaccggacag
     1681 cgcagatcaa gcagttgtta attgatgggg taaatccaga tacgttacct gtgtacttta
     1741 accggatgat ggatgttagt caggatattt tgatcattca tagtgatggc atcaataaaa
     1801 ttgttaaccg gacaaatgtc agtgatgaca tgttaaataa catacctgct agtgagacaa
     1861 tcagcgcagc aggcatttac agaagcatta ttaatgatac agagatagat gctttacgaa
     1921 ttaacattga tgaagtttcg ccatcattaa cggttactgt ggctaaattg gcttcagcca
     1981 gacataatat gcttgaacag tataaaatta atagcattat aatttgcatt gtcgccatta
     2041 tactgtgctc agtattaagt ccgttgttaa tcaggacggg gttgcgagag atcaaaaagt
     2101 tgagtggcgt aacggaagcg ctgaattata acgatagccg agtgccggtt gaggttaacg
     2161 cattaccgag agaactaaaa cctcttgggc aggcgttgaa taaaatgcat catgctttag
     2221 tcaaagattt tgagcgcctt agtcaatttg ctgacgatct cgctcatgaa cttagaacgc
     2281 caattaatgc attactgggt cagaatcagg ttacgctcag tcaaaccaga agtatcgctg
     2341 aatatcaaaa aacaattgcc ggaaacattg aagagctgga aaatatttcg cggttaacag
     2401 agaatatact gtttcttgcc agggcagaca aaaacaatgt tttggtgaaa ctggattcgc
     2461 tttctctcaa taaggaagtt gaaaatcttt tggactatct tgaatatctt tcagacgaga
     2521 aagagatttg ctttaaggtc aagtgcaatc aacaaatctt tgcggataaa attttactgc
     2581 aacgaatgtt atcgaatctt attgttaacg ccattcgata ttcaccagaa aaatcgcgta
     2641 ttcatataac cagttttctt gatgccaacg gctctcttaa tattgatatc gccagtcctg
     2701 gaacgaaaat taatgagcct gaaaaactct tccgtagatt ttggcgggga gataattcgc
     2761 gtcattccgt aggtcaggga cttggccttt ctttagtcaa agcgattgcc gaattacatg
     2821 gaggaagtgc tacgtatcac tatctcagta agcataacgt gttccgaatt acgttaccgc
     2881 aaagaaatta acatcgggct tcagtacgcc ataagttaac cgtgaaaaaa cgcagccacg
     2941 tcgtgagtat taacgtggct gcgattgatt atgcgcttac atcacaatgt aacagggatt
     3001 aactcgcgta agctgccagc atttcctgcg ccgccagttt acccaacgca ttcgctgcaa
     3061 aaggactgtc gccggtgaga agtttacggt ccttatgtac tcgcccggtg atgtcgtcat
     3121 taatgatgtt catgcccatt ttcttcaact cttcgccgaa gtaccaggtg agatgacccg
     3181 gcatatagcc aatatctggc gtttgtttgt ctgcggcgtc tgggaatgcg caaatagaat
     3241 aaccatttag tgggttatcg ctgtggcgaa gtgccagaaa agccgccgga ccgtggcaaa
     3301 gggagataac aaaacggtca tttttgattg cccactgtaa agcggcagcc acgtcctggc
     3361 tttctggtaa accaataagt gcgccatgcc caccaggaac aaagattgct gcatattcgc
     3421 tatcagcgtt gaggctggcg acaacatccg cgagtttctt cggattacgg aataacgatt
     3481 tatgctgttc aaagaatggc attacttttt catctttgtg cggcatagcc cagtattcaa
     3541 acttggtcat cagaccggaa atggtcgcca cttcgaattc gaaacctgca gcatggagat
     3601 gatacaacgg cagcaacgtt tcaatcggat ggttaccggt cgagaacagt tttccgttat
     3661 cggtcggcaa ataacgttcg tccgcggcga tcaccagaat tttatgttta ccgcgatacg
     3721 gttttggata gtccacgcca tcaagatcag agacgggact cgtatattgg ctaagcgaat
     3781 attctgaagg gaagaacgca ttatcttcag caatatcgac ctgcgggttt ttacttgttt
     3841 gaacagtcat agtgtattcc ttattgttgc ttagttaggg tagtcactat atttgcgact
     3901 gagctggtcg ctatttttaa ccaggctgaa aaattgagtc aatatatcga aagcggtagt
     3961 tggcgggcat aagtatgtaa tgatgatgct gcactggtgt tgaatattat tcacgccaaa
     4021 ctttcctcat tgagcaatgt cgtcgcaagt ggcctcaatg attttagacc attatcagag
     4081 gtccggagcg gcgggaagag atttattgct gtggaataat tgtggtactg atgattattt
     4141 atatcccgga ttggtaggga aaagcttttt tcatgcacta tttttgcaaa tatttctata
     4201 tgggaagcgg ttttgttaca gattaaattt ataaagaaat gcatttgggg tgttgggttg
     4261 aagagtagct ggtagatgtt attgtgccag caggggcagg ggaatgtgcc gtatcgatat
     4321 gctttggcaa ttgcgtgtgc cgcagatgga gatgaaatgc caataaaaaa gcctgagttc
     4381 gatgagggac gaactcaggc tggtgttatg ttttacagta acacgcctcg gcgcgttggc
     4441 tgattagaac tggtagacga taccaactgc cagttggtcg tcggtgttca gtccgtgagc
     4501 gaaaacataa tcactcttat caagcaggtt gatctgataa gcggtatata tgttcatatt
     4561 cttattgaag taataccagg taccgatttc aatatagttc agacgatcag aatctttgta
     4621 acctgtagac tggccccctg aatctccaga caaccaatat cacttattta agtgatagtc
     4681 ttaatactag tttttagact agtcattgga gagcagatga ttgatgtctt aggaccggag
     4741 aaacgcagac ggcgtactac acaggaaaag atcgctattg ttcagcagag ttttgaaccg
     4801 ggaatgacgg tctcccttgt tgcccggcaa cacggcgtgg cagccagcca gttatttctc
     4861 tggcgcaagc aataccagga gggaagtctt actgctgtgg ccgcaggaga acaggtcgtt
     4921 cctgcctctg aacttgctga tgccatgaag cagattaaag aactccagcg cctgctcggc
     4981 aaaaaaacga tggaaaatga actccttaaa gaagccgttg aatatgggcg tgcaaa
//
LOCUS       DADLSB010000227         4997 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.227, whole genome shotgun sequence.
ACCESSION   DADLSB010000227 DADLSB010000000
VERSION     DADLSB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.227"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JN331_003906"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JN331_003906"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="JN331_003907"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="JN331_003907"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY7261888.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JN331_003908"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JN331_003908"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY7261889.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADCNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JN331_003909"
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JN331_003909"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY7261890.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADVRRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3361..4695)
                     /locus_tag="JN331_003910"
     CDS             complement(3361..4695)
                     /locus_tag="JN331_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7261891.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1207 a         1418 c         1186 g         1186 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 cgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacatcag caatagcttc tgggttacag tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcaca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt accggctcgt tagtcggcac
     3121 tcgcactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aaccaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaatg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccagg ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 agcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatccctg aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacc
//
LOCUS       DADLSB010000228         4987 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.228, whole genome shotgun sequence.
ACCESSION   DADLSB010000228 DADLSB010000000
VERSION     DADLSB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.228"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1247
                     /locus_tag="JN331_003911"
     CDS             <1..1247
                     /locus_tag="JN331_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7261892.1"
                     /translation="MGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPEWDADYWFDGA
                     NYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAVDFLQQPVRAEEPFL
                     MVVSYDEPHHPFTCPVEYLEKYADFYYELGEKSQDDLANKPEHHRLWAQAMPSPVGDD
                     GLYHHPLYFACNDFVDDQIGRVINALTAEQRENTWVIYTSDHGEMMGAHKLISKGAAM
                     YDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEILPGENILAVKEPR
                     GVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDELYDRRNDPNEMHNLIDD
                     IRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPRWMGAFRPRPQDGYSPV
                     VRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(1294..1707)
                     /locus_tag="JN331_003912"
     CDS             complement(1294..1707)
                     /locus_tag="JN331_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261893.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            1816..2163
                     /locus_tag="JN331_003913"
     CDS             1816..2163
                     /locus_tag="JN331_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY7261894.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            2153..2515
                     /locus_tag="JN331_003914"
     CDS             2153..2515
                     /locus_tag="JN331_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY7261895.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            2512..3009
                     /locus_tag="JN331_003915"
     CDS             2512..3009
                     /locus_tag="JN331_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY7261896.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(3017..4201)
                     /gene="emrD"
                     /locus_tag="JN331_003916"
     CDS             complement(3017..4201)
                     /gene="emrD"
                     /locus_tag="JN331_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY7261897.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(4481..4570)
                     /gene="tisB"
                     /locus_tag="JN331_003917"
     CDS             complement(4481..4570)
                     /gene="tisB"
                     /locus_tag="JN331_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY7261898.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
BASE COUNT         1285 a         1294 c         1299 g         1109 t
ORIGIN      
        1 ctatggggcg ctactttaag gatgccggct atcacacctg ttacatcggc aaatggcatc
       61 tcgacggtca tgactatttc ggcactggcg agtgtccgcc ggagtgggat gctgattact
      121 ggtttgatgg ggcgaactat cttagcgaac tgacggaaaa agagatcagc ctgtggcgca
      181 atggcctaaa cagcgtcgaa gatttacagg cgaaccatat tgacgaaacc ttcacctggg
      241 cgcaccgcat cagcaatcga gcggtagatt ttctgcaaca gcccgtgcgc gccgaggaac
      301 ccttcctgat ggtggtttcg tatgatgagc cgcatcaccc gttcacctgt ccggtggagt
      361 atttagagaa atacgctgat ttttactacg aacttggcga gaaatcacag gatgacctgg
      421 cgaacaaacc ggaacatcac cgcttatggg cgcaggcgat gccatcgcca gtcggtgatg
      481 acgggcttta tcaccatccg ctctattttg cctgcaatga ctttgttgat gaccaaatcg
      541 gacgtgtcat caatgcctta acggcagagc aacgtgaaaa tacgtgggtc atttatacct
      601 ccgatcacgg tgaaatgatg ggcgcacata agctgatcag taaaggagcg gcgatgtatg
      661 acgacatcac ccgtattccg ctgatcatcc gttcgccgca aggggagcgg cgacaggtcg
      721 atacgccagt cagtcatatt gatttactgc cgacaatgat ggcgctggca gatattgaaa
      781 aaccagagat tctgccgggg gaaaatatcc ttgccgtgaa agagccacgc ggcgtgatgg
      841 tggaatttaa ccgctacgag attgagcatg acagctttgg cggttttatt ccggtgcgtt
      901 gctgggtgac ggatgacttt aaactggtgc tcaacctctt caccagtgat gaactttacg
      961 atcgccgtaa tgacccaaat gaaatgcata acctgatcga tgatatccgt tttgcagacg
     1021 ttcgcagcaa aatgcatgac gccttattgg attacatgga caaaattcgc gatccgttcc
     1081 gcagttacca atggagtctg cgtccgtggc gtaaagatgc acgaccgcgc tggatggggg
     1141 cgtttcgccc acgcccacaa gatggctatt cgccagtggt acgcgactat gacaccggcc
     1201 taccgacgca aggagtgaag gtggaggaga aaaaacagaa attctgacga taatgcccgc
     1261 tgatgatcac ccggcgggca ttattcaggc tgcttaaacc agcgaaccgc cggtcaggaa
     1321 ggaaaaaatc aggaatatca gcaaaatgct gcacacgatg ccgataccaa aaaccagcgc
     1381 gccgcgaatg ccccagcgat tttccttacg cataccgcca ataaatgcgc aaagtagcgc
     1441 agcaatggcc agtgcgatac cgctttgatc gaggatttta tcgatatcca tgtcctcttt
     1501 tttctccaca gtggtaattt tttcgccttt tattccggcg agaagacctc ttttaatttc
     1561 agccgttttg tccgccacga tgctttcaag cgttggcggt ggtgcggaaa aagggccgaa
     1621 tgaaaagtgg ataatcccca tcattaaagc gatggcaccg aacagcatgc ccaatgaact
     1681 gattttattt tgcgctatga tacccatctg acctccttgt agcggatggc aaaagcatat
     1741 caggaaacgg gcggacgaaa ttcttaaaaa aaaccgctaa agatagagcg ttagtgaaat
     1801 tgcaaaggag caacaatgaa aatttcccgc cttggagaag cgcccgatta ccgcttctcg
     1861 ctggctaatg agcgtacttt tctggcgtgg atccgtaccg cactaggatt tctggcggcg
     1921 ggggttgggc ttgatcaact tgcgccagat ttcgccacgc ccgtcattcg tgaactgctg
     1981 gcgttgttgt tgtgtctgtt ttccggcggg ttagcaatgt atggctacct gcgctggtta
     2041 cgcaatgaaa aagcgatgcg tctgaaagag gacctgccct acaccaacag cttattaatc
     2101 atcagcttaa tattgatggt tgtggcggtg atcgttatgg gactggtgtt gtatgccgga
     2161 tagccgtaaa gccagacgca ttgccgaccc agggctgcag ccggagcgca catcactggc
     2221 gtggtttcgt accatgctgg gctacggcgc gttgatggcg ttggctatca aacacaactg
     2281 gcaccaggcg ggcatgttat tctggatttc gattggcatc ctcgccatcg tggcgctgat
     2341 cctctggcac tacacccgta atcgcaattt aatggatgtc acgaatagcg atttttccca
     2401 atttcacgta gtccgtgaca aatttttgat ctccctcgcg gtgttatctc tcgcaatact
     2461 gtttgctgta acgcatatac atcaacttat cgtatttatt gagagagtcg catgacagga
     2521 agtcaggtca ttgatgctga agaggacagg cataagctgg ttgtagagta caaggatgct
     2581 ctgcaacctg ccgattttta tcacaatttc aaacagcggg gcatccgctc cgtacagctc
     2641 attccccatc ttgaatttga tgaccggggc gatctgacgg ccgcttcggt gacagcagag
     2701 ctgtggggga aatttttaat tgccctgttt gagtgttggg tgcgggcaga tatcagtcgt
     2761 atctcgattg agctttttga cgccacctta caaaaatggt gtggcagcga aaacccgcaa
     2821 ccgcgccgtg actgccaggc gtgcgactgg catcgcctgt gcccgcacgc tcggcaggag
     2881 acgccagaca gcgtgctttg tgcgggctat caggcgtttt attcttactc cgcaccgcat
     2941 atgcgggtga tgcgtgatct gataaagcag catcgttctc ccatggagct gatgacgatg
     3001 ctgcggtgac gtgcgcttaa acgggctgcc cctgatgcga catccgcgtc gccagcggca
     3061 gccagcacag cacgatcaac aatcccatta aggtcatcaa caaccccagg ctgccctgac
     3121 cggtttgcgg caacatcgca gagagcgacg ccagcacgcc ggaaccaatg ttttgcagac
     3181 cgccgaccag cgcaccagcc gtgcccgcca ggaaggggaa cggctccatc gcgccgctgg
     3241 tcgccagcgg aaacagcatc ccggcaccga aaaagaacag cgcggcggga acgagcagcg
     3301 tccagacatt catcacgcca aaccagtcgg ggatccacat cagcaagcca gccagcaggc
     3361 agcagataac cgactgccac atcaacgttg agaagcgttt attaggacgc ccggcaaacc
     3421 atgccccaaa aaatgccgcc ggaatcggca gaataaacaa aatactgacc gtcatactgc
     3481 tcagccctaa caccgcgccc atcagcacgc cggagcaggc ttcaaaggca gcaatcccgg
     3541 ccagaccgcc aatcagcatc agcaaataac agttaaaacc gctgttaccg aaaagcgttt
     3601 tataactggt aagcaggcgc gtgcgcggtg catcgaccgg acgcgtttcc ggcatccagc
     3661 gggccatact gaaggtcaca ccagcacaaa gaaccaacaa aaagagataa caggcgcgcc
     3721 agttccacat cgtatccagc agaccgccga ttagcggtgc gagcaacgga ctgacgagaa
     3781 tccccatgtt taacaggctg ttagcatggc gcaactgtgt ccgttcatat aaatcacgcg
     3841 gcaaagtacg cgccattacg ccgccaacgc cggtgcccat cccctgcatc gcgctggcgg
     3901 caatcaatac cgtcaaactg gaggtcgtga ccgcgaccag cgttgccagc ataaaaatgg
     3961 acattccgac gaggatcact ggtcggcgac cgacacggtc ggatatcggg ccataaaaca
     4021 gctgtgagac accgtaagtc agcagataag cgcccattac gctctgcacc gccccttcac
     4081 ggacgttgag atcgcgcgcc atatcggcaa tagctggaat ataaatggtt tgcgccatct
     4141 gaccgacggc cacgagtaat accaacatca ataacaaatt gacgtttctt tgccttttca
     4201 ttatcacgga tgcttttata aaaataatta gaataagtca ctacgcattc ccataaatgc
     4261 gggagaggaa tctatcacat tgatgaatgt tagccagata tacgccagga atggggaatt
     4321 gtttagcggc agaatatgta aacaaaagcg gcaataaatg ttgccgggag ggatggtagg
     4381 gcggataaga tgcgtcagca tcgcatccga ctccaacccg cacgctaaat ataaaagggg
     4441 agcggttacc cgctcccctt tggtgcgact tgaatctgaa ttacttcagg tatttcagaa
     4501 cagcatcaag cagttgcagt gctgcaacaa tgagtttgag gataagaatg gcgatatcca
     4561 ccaggctcat acgcgtctcc tgtggttcag gagcactgcc agctgacgta cctttccgct
     4621 gcctgttgcc agctgttgtg cacgcggtat ctcaaggaga aacgttgtgc ttgttgacat
     4681 aacacagtgt gctcacgcgg ggttaaggcg ctgacggcac cacccgtttc agccaggact
     4741 tagtgcgccg ggtaacgatg cggcccccgc ataacacatt gcgtacagtg atattataga
     4801 agtttactgt ataaataaac agtaatattt ggacaaaacg caaactgtga tcgatactca
     4861 actatgcact aaatacgtca aaattcgtgc cgaaattgcg cgttctgcgc ggaacacgta
     4921 tactttcagt gttgacataa tacagtgtgc tttgcggtta ccagccgcag gcggctgacg
     4981 aaacctc
//
LOCUS       DADLSB010000229         4938 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.229, whole genome shotgun sequence.
ACCESSION   DADLSB010000229 DADLSB010000000
VERSION     DADLSB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.229"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(109..1848)
                     /gene="flhA"
                     /locus_tag="JN331_003918"
     CDS             complement(109..1848)
                     /gene="flhA"
                     /locus_tag="JN331_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY7261899.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGMLVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            1793..2578
                     /gene="lafU"
                     /locus_tag="JN331_003919"
     CDS             1793..2578
                     /gene="lafU"
                     /locus_tag="JN331_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY7261900.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQRLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2649..3704
                     /gene="dinB"
                     /locus_tag="JN331_003920"
     CDS             2649..3704
                     /gene="dinB"
                     /locus_tag="JN331_003920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY7261901.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3756..4049
                     /gene="yafN"
                     /locus_tag="JN331_003921"
     CDS             3756..4049
                     /gene="yafN"
                     /locus_tag="JN331_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY7261902.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            4052..4450
                     /gene="yafO"
                     /locus_tag="JN331_003922"
     CDS             4052..4450
                     /gene="yafO"
                     /locus_tag="JN331_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY7261903.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            4460..4912
                     /locus_tag="JN331_003923"
     CDS             4460..4912
                     /locus_tag="JN331_003923"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7261904.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
BASE COUNT         1204 a         1272 c         1310 g         1152 t
ORIGIN      
        1 ctgatgcgac gctggcgcgt cttatcatgc ctacaaactt gtgcctgaac ggtaggccgg
       61 ataaggcgtt tacgccgcat ccggcaattg tgcaccgatg ccggattctt acatcaacgc
      121 ccccataatc ttcaactcca gctcatccgg cacttcgtta tacgacagca catgcagccc
      181 cggcgcaaac aaccttgcat aacgcgccag caaagggcgt agctgcggcg gcaccagcag
      241 caccggatct ttccccgccg ctttcatctg ctctttcacc tgtggcatcg tgctctggaa
      301 ctggttgagc atattcgggt caaccggcac gctgtcgagc atcactttgc cggcctgttg
      361 cgcctgattc actacgttgg tcagcagatt ttccagctca ttattcagcg tatacaccgt
      421 cagctcctgc ttgcgaacga acggatgagt aatgctgcgc cgcagcgcca ggcgcacatc
      481 ggccgccagc agaatatgat ctttagtcac cgtgctactg gcgaccagca cggtggcgat
      541 ggtgacgata tcgcgcaggg aaacgccttc ggtcagcagc gcccggtaca ctttcagcaa
      601 ttggctgtaa ttgagcgccg cgcttaaatc ctccgccagg cgcggtgcca tcgacgacaa
      661 acggttatgc aactgcgtga tgtcatcata gttaaacaaa tcaggaatat agctgcgcac
      721 aatcttgttc acatgggtgg caatcacact ggcgctgtcg atcacctgat accccatatt
      781 cagagccttc gctttctgcg caggctggat ccaggtgacc agcatcccgt acgccgggtc
      841 gttccccagc acgccgtcaa tctcgccgta ggtttcgctg gagggcagcg ccatcagttt
      901 atccgccgga atatccgctt catcagcctt aatgccgttg atgaaaatag cgtactggct
      961 gggcttgagg cggaagtttt cccgaatgcg gatctccggc agcagcacgc cgttaccgtc
     1021 ggaaatcacc tgccgaacgc cgcgaatccg ctgggtgagc gggttgccct gcgctttgtc
     1081 caccagcgcc accagcttgt agccgaggct taagctgatg ggctcgatca gcggaatagt
     1141 ttcccaactg acctgttgtt cgctggtttc agtgatggtg cgggtcagcg tttcgaggct
     1201 tttctcttcc gcctccgccg cctgcgggcg tttgctcatc cgccagccgg taaagccaag
     1261 cagggcgctg aacagcaaaa acggcagatg cggcatcccc ggcaccaccg ccagcacgaa
     1321 catgatcccg gtggcggtgt agagcaccga cgggcttgcc agcaactggt ggcgcacgtc
     1381 atgggcgata tcgccgttgt cgctgacgcg ggtgacgata atcgccgccg cggtggagag
     1441 cagcagggaa gggatctgcg ccaccaggcc gtcgccgatg gtcatcagca cgtattgctg
     1501 gaaggcggcg tcggcgctca ggttgtattt gaagatgccg atacagacgc cgccgctcag
     1561 gttgatcgcc agaatcatca tcccggcgat ggcgtctccg cgcacaaact tcgatgcccc
     1621 gtccatcgcg ccgtagaaat cggcctcgct ggcaacatct ttacgccggg tttgcgcctg
     1681 cgcctggttg atcaatccgg cgttaagatc ggcgtcaatc gccatctgtt tgccgggcat
     1741 cgcgtccagg gtaaagcggg cagaaacctc ggaaatacgc tcggcctctt tggtgacgac
     1801 gataaagttg attgtcaaca gcgtcagtaa atccgagcgt gaaagcatta tcgccgcgct
     1861 gcacgggcag tcgattttta acggtggtgg gctgtcgccg ttaaataaaa tcagcccgtc
     1921 tcatccgcca aaacctgcca ccgtggcggt gccggaagag acggagaaaa aagcgcgtga
     1981 tgtgaatgag aaaacggcgc tgctgaagaa gaaatccgcc accgagcttg gcgagttggc
     2041 aaccagtatc aacaccattg cccgtgatgc gcatatggaa gcgaatctgg agatggagat
     2101 tgttccccag agattacgcg tgctgattaa agacgaccag aatcgcaata tgttcgaacg
     2161 cggcagcgcg cagattatgc cgttctttaa aactttgctg gtggagctcg cgccagtgtt
     2221 cgactcgctc gataacaaaa ttattattac cgggcatacc gatgcgatgg cctacaaaaa
     2281 caatatctac aacaactgga acctttcggg cgatcgcgcg ctttcagctc gtcgggtgct
     2341 ggaagaggcc ggaatgccgg aagataaagt gatgcaggta agcgcaatgg cggaccagat
     2401 gctgctggac gcgaagaatc cgcaaagcgc gggcaatcgg cgtattgaga ttatggtgct
     2461 gaccaaaagt gcgtccgata cgctgtatca atactttggt cagcatgggg ataaagtggt
     2521 gcagccgctg gtgcaaaagc tggataagca gcaggtgctt tcgcagcgaa tgcgctaaat
     2581 gctgaatctt tacgcatttc tcaaaacctg aaatcactgt atactttacc agtgttgaga
     2641 ggtgagcaat gcgtaaaatc attcatgtgg atatggactg ctttttcgca gcggtggaga
     2701 tgcgcgacaa tcccgccctg cgcgatatcc ctattgctat tggcggcagc cgcgaacgtc
     2761 ggggggtgat cagcaccgcc aattatcccg cgcgtaaatt tggcgtacgt agcgctatgc
     2821 ctactgggat ggcgctcaaa ttatgcccac atctcacctt gcttccgggg cgctttgacg
     2881 cctacaaaga agcctcaaat catatccgtg aaatcttctc gcgctacacc tcgcgcattg
     2941 aaccgttgtc actggatgag gcttatctcg atgtcaccga tagcgtccat tgccacggtt
     3001 ctgcgaccct catcgcccag gaaatccgcc agacgatctt caacgagctg caactgacgg
     3061 catctgcggg cgtggcacca gtaaagtttc tcgccaaaat cgcctccgac atgaataaac
     3121 ccaacggtca gtttgtgatt acgccggcag aagttccggc atttttacaa accttaccac
     3181 tggaaaaaat ccccggcgtc ggcaaagtct cggcggcaaa actggaagcg atggggctgc
     3241 ggacctgcgg tgatgtacaa aagtgtgatc tggtgattct gcttaaacgc tttggcaaat
     3301 ttggccgcat tttgtgggag cgtagtcagg ggattgacga gcgcgacgtt aacagcgaac
     3361 ggttacgaaa atccgtcggc gtggaacgca cgatggcgga agatattcat cactggtctg
     3421 aatgtgaagc gattatcgag cggctgtatc cggaacttga acgccgtctg gcaaaggtaa
     3481 aacctgattt actgattgct cgccaggggg tgaaattaaa gttcgacgat tttcagcaga
     3541 ccacccagga gcacgtctgg ccgcggctga ataaagctga cttaatcgcc accgcgcgta
     3601 aaacctggga tgaacgccgc ggcgggcgcg gtgtgcgtct ggtggggctg catgtgacgt
     3661 tgcttgaccc gcaaatggaa agacaactgg tgctgggatt atgatgtata ctattatgta
     3721 tattctggtg tgcattatta tgagggtatc actgtatgca tcgaattctc gctgaaaaat
     3781 cggtcaatat cactgagtta cgtaaaaacc cagctaaata ctttattgat caaccagttg
     3841 cggttctttc taataatcgc cccgcaggat atctcttaag tgccagcgca ttcgaagcgt
     3901 taatggacat gcttgctgaa caagaggaga aaaagcccat aaaggcgcgc tttcgtccaa
     3961 gtgctgcaag attagaggaa attacacgcc gcgctaaaca atatcttaat gatatgacgg
     4021 atgatgattt caatgacttt aaggaataag gatgcgggta ttcaaaacaa aacttattcg
     4081 cctgcaactt acagcagagg aacttgatgc gttaacggcg gattttattt cctataagcg
     4141 tgacggtgtt ttgccagata tatttggtcg cgatgcactc tacgacgact cgtttacctg
     4201 gccattaatc aaatttgagc gagttgctca tattcatctg gcaaatgtga ataatccatt
     4261 tccgccacag ttgcgccaat tcagcagaac gaatgacgaa gcgcatttgg tatattgtca
     4321 gggtgcgttt gatgagcaag catggttgct cattgccatt ctgaagcctg aacctcataa
     4381 actggctcga gataacaacc aaatgcataa aattggaaaa atggcagaag cgtttcgcat
     4441 gcgtttttga atttatatta tgaataacat acaaataaga gattatcagc ctggcgattt
     4501 tcagcaacta tgcgctattt tcattagagc ggttacgatg accgtcagtc agcattattc
     4561 accacaacaa attgccgcct gggcgcagat tgacgaatct cgctggaagg agaaactcgc
     4621 gaaatcacaa gtgcgggttg cagtcattaa tgcaaaactg gtcggcttta ttacctgcgt
     4681 tgaacactat atcgatatgt tatttgttga ccctgaatac acccgccgtg gggttgccag
     4741 cgctttgtta aaacctttga ttaagtctga atccgaactt acggtggacg caagcataac
     4801 cgcaaaaccc ttttttgaac gttatggttt tcagacagtt aagcagcagc gcgttgaatg
     4861 ccggggagcg tggtttatta atttttatat gcgttataaa ccgcaacatt aaatccagct
     4921 tgcaatgaaa ataacgcc
//
LOCUS       DADLSB010000230         4928 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.230, whole genome shotgun sequence.
ACCESSION   DADLSB010000230 DADLSB010000000
VERSION     DADLSB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.230"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(403..1494)
                     /locus_tag="JN331_003924"
                     /pseudo
     CDS             complement(403..1494)
                     /locus_tag="JN331_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1526..2071
                     /locus_tag="JN331_003925"
     CDS             1526..2071
                     /locus_tag="JN331_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7261905.1"
                     /translation="MKDVKVMPESIPAGYEVLQELDELDSLLIIDLGGTTLDISQVMG
                     KLSGISKIYGDSSLGVSLVTSAVKDTLSLARTKGSSYLADDIIIHKKDNNYLKQRIND
                     ENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHVMVIGGGAELICDTVKKNTQIRNE
                     RFFKTNNSQYDLVNGMYLIGN"
     gene            2074..2425
                     /locus_tag="JN331_003926"
                     /pseudo
     CDS             2074..2425
                     /locus_tag="JN331_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein stbB"
     gene            complement(2575..2748)
                     /locus_tag="JN331_003927"
     CDS             complement(2575..2748)
                     /locus_tag="JN331_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261906.1"
                     /translation="MLSGQIFCIPLNNLVGDKINYDEITKITARDWRQYRAPGWQITH
                     QKRYCQTLRTHQF"
     gene            complement(2801..3076)
                     /locus_tag="JN331_003928"
     CDS             complement(2801..3076)
                     /locus_tag="JN331_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7261907.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(3076..3360)
                     /locus_tag="JN331_003929"
     CDS             complement(3076..3360)
                     /locus_tag="JN331_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAY7261908.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDHPLPVPR"
     gene            3812..4063
                     /locus_tag="JN331_003930"
     CDS             3812..4063
                     /locus_tag="JN331_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7261909.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGSFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            4032..4322
                     /locus_tag="JN331_003931"
     CDS             4032..4322
                     /locus_tag="JN331_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY7261910.1"
                     /translation="MNAHWSSKKSNFFRKNIKLLTKYLFFESQGIPDKVDIVSRLKTY
                     GYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(4422..4607)
                     /locus_tag="JN331_003932"
                     /pseudo
     CDS             complement(4422..4607)
                     /locus_tag="JN331_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1276 a         1192 c         1184 g         1276 t
ORIGIN      
        1 tatacgataa ttagcatact tataacaccg taagtaaatt acatgaaagc aggcacagcg
       61 tgatccgatt tttctgcgta taacgatttt ccggaagtgt aatacggttg cgccccgggc
      121 cgggcaccct tttctgttgt tgtgtacggg cccacagaga atggtaccgg aggcgggctc
      181 agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta ctgacacggg tattggtgac
      241 tggtgatgaa gtactgcccc gggtatgatt tcaggctgat tcaggttctg attctctgtc
      301 agtccgcgtg gttgctggcc tagctgattt cctgttgtca ggcgttcaac aaccgactgc
      361 aggttatagc ccggttcacg aacacctgac ggcgggtatc actcacccgg tgaaggtaaa
      421 tccctcaaga ggcagcaact ctgccagtcg ctcctccggc cattccggca gacgcgtcag
      481 gacgtccgtc agccaggcat gtggctccag accattacgt tttgctgttt ccagaaggct
      541 cattatctgt gccgcccggt ttccggccat cagtgaaccg gcgaacagcc acgctttccg
      601 tcccataaca accgggcgaa tggcacgctc gcagatattg ttgtccaggg gcaccgctcc
      661 gtcacccagg aagcagctca gttcatcgcg ccggttcaga atatagttaa tcgccttatt
      721 taatggcccg tcgggcggac agcacggctc ctgctcttca agccaggcaa acaggtcatt
      781 cactatcggc cgggagtacc gttatcgcca ctgccgtatt ttttccacag ggcgctcacg
      841 gatgagtttc tcaatgcggt aaagacccgc gatttttctc agctccacgc ctgcacgggg
      901 ctccctgctg gccttataca gatttgcgaa gccgcgccct gcgtgactcc agcaacaggc
      961 tgatgtgatg ccgggatgat tatccgccag agatttgtac acgctgtatc cgtcactcac
     1021 cagcgtaccg ccacaccagc actgcagcca ggtctcaggg tatctgaggg cccgtccggt
     1081 ctgcgagtca aaacagacga ccggcggacc gcttctttca ccgctgacgt aagcacacag
     1141 ccagccggag caggatttac cgcctttccg ggtatcaagg agcctcaggg acgtttcatc
     1201 tgcatgaata acatcccgtg tcagaagctc atggcgtagc agtttcgcca gcggggccag
     1261 tgcagctccg gcaacaccga ccatatccgc cattgtactg acggggagct ccacacccat
     1321 tcgggagaaa atgtgctgct ggcgatacag gggcagatgg tcggtatatt tgctgatgac
     1381 aacctgtgca attaccgacg actcaacagc actttcaggg aggatatgcg ccggcatttt
     1441 cccgctgaac actttctcac agcagggaca gctgtactgc ggacggatat agcggattgc
     1501 attaaatggt ggggatacat tcacaataaa agatgtaaaa gtcatgcctg aatctatacc
     1561 ggcaggttat gaagttctac aagaactgga tgagttagat tctttattaa ttatagatct
     1621 cgggggcacc acattagata tttctcaggt aatggggaaa ttatcaggga tcagtaaaat
     1681 atacggagac tcatctcttg gtgtatctct ggtcacatct gcagtaaaag ataccctttc
     1741 tcttgcgaga acaaaaggaa gtagctatct tgctgacgat ataattattc acaaaaaaga
     1801 taacaactat ctgaagcaac gaattaatga tgagaacaaa atatcaatag tcaccgaagc
     1861 aatgaatgaa gcacttcgta aacttgagca acgtgtatta aatacgctca atgaattttc
     1921 tggttatacg catgttatgg ttataggcgg tggcgcagaa ttaatatgcg atacagtaaa
     1981 aaaaaacaca cagattcgta atgaacgttt tttcaaaacc aataactctc aatatgattt
     2041 agttaacggt atgtatctca taggtaatta ataatggaca agcgcagaac cattgccttc
     2101 aaactaaatc cagatgtaaa tcaaacagat aaaattgttt gtgatacact ggacagtatc
     2161 ccgcaagggg aacgcagccg ccttaaccgg gccgcactga cggcagatct ggccttatac
     2221 agacaagatc cccggccccc catttctttt atgtgaactg ctgacgaaag aaaccacatt
     2281 ttcggatatc gtgaatatat tgagatcgct atttccaaaa gagatggtcg attttaattc
     2341 ttcaacaatc actcaatcct cttcacaaaa agagcaaaaa agtgatgaag agccccaaaa
     2401 gaacgtgatg aagctaataa actaattcaa ttattattga gtttccttta tccactatca
     2461 ggctggataa aggaaactta atcaagttat tttcttgcca atcattacat aaaataaaca
     2521 gggtcgttat tatgaaatta atccacggtg cttatgaaaa agcggctcca ataatcaaaa
     2581 ttggtgagtt cgcaatgtct ggcaataacg tttctgatgg gttatttgcc agcccggagc
     2641 gcgatattgc cgccagtcac gagcggtaat tttcgttatt tcatcgtaat ttattttatc
     2701 tcctacaaga ttatttaatg gaatgcaaaa tatctggcct gataacaatg ctccagaatt
     2761 aatacctgca ggctatgtta aagttgcctc cacacccaat ctacctgttc tctcgtgtgt
     2821 gccacaggcg caatacataa atagtctggc cagtaagttc gtaacggatt tcgtactcgc
     2881 cagtaaaaat ccgtctgacc tccctgggtt caaactgaaa caactgttct cccatacgtg
     2941 gatgagttaa cagaatgacc ggcgcctgtg tcagggactg caccgttctg gcggcagcag
     3001 gtttactggc cagcaccaga aaatcatata accgcgccaa atcagaaagt gccttactgg
     3061 tccacttcag ttccatcagc gtggcaccgg taacggatgg tcagtactga ggctgtccgc
     3121 ccaggcctgt acagcctgat ggtcgataac ctgtccggat gtcacatcgt ccagggcttc
     3181 cagcgtcagg cgattacgct cctcctcttg ggcaagccat gcagaaagcg cctgtttgat
     3241 aacccagccc cgggagcgtt ccagtctggc ggccatctgg tcgactttat cagccatagg
     3301 taatggcaca tgcgcggtaa ttacttttgt tgcttgtgcg gtattgtttt tcatttgcat
     3361 acccgattag ttgttaatca tatttaatca tagtgattac tttcagacgg aacaaggcaa
     3421 ataatgcgag tcagcgcata taaggcggca tacggtggtt acatgtagca caggtgtaaa
     3481 accgccgaag cccggcgcaa gccggtacgg tcgtccgaat atcaccagtc cccctccggc
     3541 tctgcgaggc catgcggcct gtcagcgatg aaaatgcctc tgtgcgcggt cggagtggtc
     3601 ccgacaaggg tttacccgca gtcgaggcgt atctccgcgt tagcgggccg tgagggccgc
     3661 ttacgagcgt gtatgagaga cgtacagtag ttttgtccgc tcacaccagt acaaaccagt
     3721 cgaaataccg gtaacgacga ttactcttgt ctctgatgcc ggtggactac gagattttat
     3781 ttactggcgt cctgatgcgg tcaggactgg tatggaacct gtttatgtga tacttaatgc
     3841 tcttctggat tccgggcgat ttacgcgtaa acttattctg ttagggctgt caggctcatt
     3901 ttcctatatc ttcggctcaa ttgttgcgac actgggaatg ggacttgttg tcgattatct
     3961 gggatggggg gccacattta tcgtactgat actgtctgct gtttttgcca tcattttcac
     4021 actgatgagt cgtgaacgct cactggagtt cgaaaaagag taattttttc aggaaaaaca
     4081 taaaattact gactaaatat ttattttttg aatcgcaggg cattccggat aaggttgata
     4141 tcgtgtcacg gctgaagacc tatggataca gtatttctgg tgtggagacg gatgacggat
     4201 ataaggcgct tgtccgcgct tttcagcttc atttccggca gaaaaattat gacggcataa
     4261 tggatgctga aaccgctgcc atcctgtatg cattgctgga gaagtatttc ccgggtaaat
     4321 aaggagtgag tcgtcagagc tgcgatatat ccccttcctc catgggctgt ctcttataca
     4381 caaatcccca cccggggatt tgtgatgtca gatgtcccct gtaaaactat gttttcattg
     4441 cgctctatga agctgagtga gcgtaacgac tggagctgct tctgcggtgc tccggtggcg
     4501 aacgtgaagt catactcttc gaacattttc accgccggga aggctaccat tcgggtatac
     4561 atcgcctgtt tacgttgatg acgtgccagt ttttcttcat gaagcatgat gcttcaagaa
     4621 gggcactgtt gcaaagttat cgatgaggta gctgaccatc actgctgtcg gaatcgatct
     4681 tgcccttctg tttccagtcg cctcagcaaa ctatttttca gttaccacct gtcctgcggt
     4741 aagcgtaaac cgaccaccgt atgtagccat tagacaagaa ttggtaactt agacgcccat
     4801 ctgacataga cgaacaccta agtatggaat tacaggactg gcgaaaagaa cctcgtaaaa
     4861 gtaagcgccc catcagcgac gtcttgtgaa aattggtaag cgccccatca gcgacgtctt
     4921 gtgaaaat
//
LOCUS       DADLSB010000231         4922 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.231, whole genome shotgun sequence.
ACCESSION   DADLSB010000231 DADLSB010000000
VERSION     DADLSB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.231"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..785
                     /gene="msrP"
                     /locus_tag="JN331_003933"
     CDS             <1..785
                     /gene="msrP"
                     /locus_tag="JN331_003933"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HAY7261911.1"
                     /translation="NLPLTPADKVSGYNNFYEFGLDKDDPAANAGSLKTDPWTLKISG
                     EVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFPLHKLLALAEPTSNAKY
                     VAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLTLMTVGVYGKALPPQNG
                     APVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYGFYANVNPHVDHPRWSQ
                     ATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF"
     gene            786..1421
                     /gene="msrQ"
                     /locus_tag="JN331_003934"
     CDS             786..1421
                     /gene="msrQ"
                     /locus_tag="JN331_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAY7261912.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            1679..2329
                     /gene="zinT"
                     /locus_tag="JN331_003935"
     CDS             1679..2329
                     /gene="zinT"
                     /locus_tag="JN331_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY7261913.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2999..3088)
                     /locus_tag="JN331_003936"
     tRNA            complement(2999..3088)
                     /locus_tag="JN331_003936"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3052..3054),aa:Ser,seq:cga)
     gene            3182..3979
                     /gene="mtfA"
                     /locus_tag="JN331_003937"
     CDS             3182..3979
                     /gene="mtfA"
                     /locus_tag="JN331_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY7261914.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEIAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            4080..4155
                     /locus_tag="JN331_003938"
     tRNA            4080..4155
                     /locus_tag="JN331_003938"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4113..4115,aa:Asn,seq:gtt)
     gene            4317..>4922
                     /locus_tag="JN331_003939"
     CDS             4317..>4922
                     /locus_tag="JN331_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7261915.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYP"
BASE COUNT         1303 a         1137 c         1120 g         1362 t
ORIGIN      
        1 ataacctgcc actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg
       61 ggctggataa agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac
      121 tgaaaatcag cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc
      181 gcttcccgct ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg
      241 tgccgtggat tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg
      301 cgaagtatgt cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc
      361 gctttatcgg cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa
      421 tgcatccgct cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg
      481 gcgcgccggt gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg
      541 tcagtattaa gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg
      601 aatacggttt ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta
      661 ccgaacgatt tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt
      721 ttaatggtta cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt
      781 tctaaatgcg tctgacagca aaacaggtga tatggctgaa agtttgcctg catcttgccg
      841 gattgttgcc gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc
      901 cggtgaaaga tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct
      961 tgttaatcac acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc
     1021 tgttaggctt atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg
     1081 agttgggcgt gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa
     1141 cgttaggaat tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga
     1201 tgcagcgaaa actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga
     1261 tcctggcccc aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct
     1321 acgctgggct ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca
     1381 accggttacg caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac
     1441 ttcgcgttat cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc
     1501 aagttatgtt tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata
     1561 agatagttca atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt
     1621 ttcatatgtt acaatataac attacacata atgtacatta actctggagg aaattgtttt
     1681 ggcgattcgt cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc
     1741 cttttcgcat ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc
     1801 taatggtgtt tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt
     1861 ctggcaatcc gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa
     1921 agcggatgca gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta
     1981 tccgacagat atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa
     2041 tgaaacaaca tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg
     2101 caagaaaggc gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata
     2161 tatacaattt agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat
     2221 gggtaatgat tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc
     2281 ctatcagttg agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc
     2341 tgacttcagt tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa
     2401 ataagtaaga caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt
     2461 agaaaaaaac tgagaaaaag gcgaacaata aatacacaac tcagccatac agactggcag
     2521 cgcaaatgtt tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta
     2581 attttggtgt ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg
     2641 ggaaattcaa caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg
     2701 cgttcctttt ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca
     2761 actttgcggc agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac
     2821 gactgaataa ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc
     2881 gctgaatgtc acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta
     2941 cgattgttct gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaagtg
     3001 gcggagagag ggggatttga acccccggta gagttgcccc tactccggtt ttcgagactg
     3061 gtccgttcag ccgctccggc atctctccgt tcagatggtt gccatgatgc caggaaattt
     3121 ggcattttaa cagtccctgt ccgtgcaatt ttgttcaagt gacgagtttg cgagcaaaac
     3181 gatgattaag tggccctgga aagtacaaga atcagcacat caaactgccc ttccctggca
     3241 ggaagcacta tcgatccccc ttttaacgtg tctgacagaa caggaacaaa gcaaattggt
     3301 cgctcttgcc gaacgttttt tacagcaaaa acggcttgtt cctttacagg gctttgagct
     3361 gaattcatta agaagctgcc ggatagcact tctattttgc ctgcccgttc tggagttagg
     3421 actggaatgg ctggatggtt ttcatgaagt cttaatttat cctgcgccat ttgtggtcga
     3481 tgatgaatgg gaagacgata tcggtctggt gcataaccaa cgtattgttc agtcaggtca
     3541 gagctggcag caagggccta tcgttttgaa ctggttggat atacaagatt cttttgatgc
     3601 ttctggtttt aacctgatta ttcatgaaat cgctcataag ctggacaccc gtaacggcga
     3661 tcgcgccagc ggagttccct ttatttcgtt gcgtgaggtt gctggctggg aacacgatct
     3721 tcatgctgca atgaacaaca ttcaggaaga aatcgaatta gttggtgaga atgcggcgag
     3781 cattgatgct tatgctgcca gtgatcctgc tgaatgtttt gccgtacttt ctgaatattt
     3841 ctttagcgcc ccagaacttt ttgctcctcg tttcccttca ttgtggcaac gtttctgtca
     3901 attttatcaa caagatcctt tgcagagact gcatcacgct aatgatacag actcgttttc
     3961 ggcgacgaat gttcattaat taacaacttt gcagattaat taaccaattg aaatggctta
     4021 tgaaatttag tgttgacaga caaggtaccg ctaagtaata tgcgccccgt tctcacgatt
     4081 cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc
     4141 cagtcagagg agccaatttt ctgttttcat gcatccttgc gaatctttat ctgatgttga
     4201 ttcaacaggt tagtgtgaaa actcttctcg gtgcattttg attttaccct acgcatcggg
     4261 aaaaattggt ggtcaaatct ggggtcaggt tagttcgata atggagtgac ccccatatat
     4321 cccttaccga cgcaaaaatc cgcaccctca agccttctga taaacccttt aaagtctccg
     4381 attctcacgg tctgtatctg ctggtcaagc cgggtggctc ccgccactgg tatctcaaat
     4441 accgtattag cggtaaagaa tcccgcattg cgctgggtgc ctatccagcc atctccctgt
     4501 ctgatgcgcg acagcaacgt gaaggtatcc gtaaaatgct ggcgctgaat atcaacccgg
     4561 tacagcagcg ggctgctgaa cgtggctcac gaacaccgga gaaagttttt aaaaacgtgg
     4621 cgctggcgtg gcataaaagt aacaggaaat ggtcgcagaa caccgccgac cgtctgcttg
     4681 ccagcctgaa caatcacatc tttccggtca tcgggaacct acctgtatca gaacttaaac
     4741 cccgtcattt cattgacctg ctgaaaggga tcgaggaaaa aggtctgctg gaggttgcgt
     4801 cccgcacacg gcagcacctg agtaacataa tgcgccatgc ggtccatcag gagttaatcg
     4861 atacgaaccc tgcagcaaac cttggcggcg tgaccacacc tcctgtcaga cggcactatc
     4921 ct
//
LOCUS       DADLSB010000232         4901 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.232, whole genome shotgun sequence.
ACCESSION   DADLSB010000232 DADLSB010000000
VERSION     DADLSB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.232"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            524..1087
                     /locus_tag="JN331_003940"
     CDS             524..1087
                     /locus_tag="JN331_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7261916.1"
                     /translation="MKVSKIALAMGLSMALVSGFANAADSGTTPPANTAAEGGHGHGT
                     VHFKGEIIDAPCSISPESADQTVDLGQVANSALKDKGTSSPVAFNIKLQNCDISTYKT
                     VTATWSGTPDVNMPEAWGITGTASGAAIVLRDASEKEITLGQDTAPTTLTADDTTIAM
                     SAFLKGDGEAIVPGAFTGAADFVLSYQ"
     gene            1157..1684
                     /locus_tag="JN331_003941"
     CDS             1157..1684
                     /locus_tag="JN331_003941"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7261917.1"
                     /translation="MVMLRIVFLLLLLVSSTGRAVSHGHGHVQMQGTIIDTACAIATG
                     DVEQGIDFGALTASELINNGRGPSLPFTVHLVNCVLDGTDIRGLNHWKDVRITFVGDA
                     DSENHFALQGDGRGAALVIADSQGVEAKPGEPMPSTPITPGSMALHYQLWLTGGKTIR
                     PGHIHTTVRYFMEYD"
     gene            1684..4281
                     /locus_tag="JN331_003942"
     CDS             1684..4281
                     /locus_tag="JN331_003942"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012786.1,HMM:NF025319.1,HMM:NF025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7261918.1"
                     /translation="MFNQKSGNNGGLNNVRLNSLTWMLLLVLGGIPAAQSQDNMSTPA
                     NSNIPDEVVEQPNITEFNTDVMDVNDRNNIDVSHFSRAGYVMPGEYPLILVVNGTEIR
                     EVTVSFAPPEDDPQGSSPCLTPEMVKLFGLKDEATKKLIWKGNGDDRCLDEKSLPGMT
                     ATGDLSTGSLKISLPQAWLEYSAPDWDPPSRGDNGVADLLFDYNLNASKSHALSEGGS
                     SATSLSGSGTAGANFGPWRLRADWQASRYSDRGYTSQSMDWSRFYLYRALPKLGARLT
                     LGESDLDSSLFDSFRFIGGSLVTDDNMLPPNLQGYAPEVTGVARSNAKVVISQQGRVI
                     YESQVAAGPFRIQELSSSVSGTLDVRVEEQDGRVQHFQVDTASIPYLTRPGLVRYKAS
                     LGKPTDWKHHVQDVSFATGEFSWGMTNGWSLFGGLVAAKKYAALAVGIGRDLLSFGAV
                     SFDVTESKATLDTGEKKMGGSYRLSYSKRFEKIDGQITFAGYRFSEKDFMSMNDFLET
                     EYDAGSRSDTAWGRQKELYTITVSKAFPDWNMNLYGNYSHRTYWNRPDADRYSLSLSK
                     YMDVGRWKGISLNLTGYRSEAWGVKDDGVYASLSIPWSDGGSLSFNGQSTTAGTTQMV
                     GWYDRIDSNNNYSLQAGTSVGNKASASGYVSHNGDLTNGTLSASYYEGNNTSLGLSLQ
                     GGMTVTRYGAALHPGAMNGGTRLMIDSGDVAGVPLSHSGVLQRTNAFGVGVIGNLNSY
                     WRNSVGVDVNALDDDMEVSHPLTELTMTESAIGYRKIDVLEGRKLMAVVKFADGSSPA
                     FGATVWRGSHQTGVVDDNGSVWLAGVKPGVTMEVREDENPVCQVGIPERLPPENTDQS
                     LLLTCQQKS"
     gene            4395..>4901
                     /locus_tag="JN331_003943"
     CDS             4395..>4901
                     /locus_tag="JN331_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015376357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7261919.1"
                     /translation="MNRNKSIKSQPFLKSLLVMALVSQSAFQVADAAVALDRTRVIFE
                     GGNKSMSLNIRNDNTKLPYLAQSWVEDEQGNKIQSPLMALPPLQRMEPGSKSLVKIQL
                     APGVEAKLPQDRESLFYFNLREIPPKSNKANVLQLALQTRVKMFYRPKALAKNQAMNE
                     TPWQEQLTL"
BASE COUNT         1292 a         1019 c         1301 g         1289 t
ORIGIN      
        1 ggacggtgaa agcaagaagc tattaggcgc tataggtctt ttgacggttt ttgaatttcc
       61 cctcctgata tctctgttat atttttgtga atttctccaa tccaaatcaa agtcccaata
      121 agtcatggat gcatggttaa aataactatc atttgtgtaa cctttggtgc ttataacttt
      181 tctttattct tttttcagga ttacctggag ggattgacca aaaaaacatt cattgaatga
      241 tatttttcta attaatgagg ttttctaagc tcgttgaaat aaaaaatata ttttaatcat
      301 gggtttgcat gcgtttcttt cgcctttttt acacaagttg acatcgaact cagcaactat
      361 aagaatagcc acatcaccag acggtgaccg ataaaggaaa taccgtttcc atatttaaat
      421 gaattggaaa atatcaccga tttgaagtcg actcgttcgg gtaattatta atactgaatc
      481 tttaatgctt atttcttaag cttgttaaca acgggaatga ataatgaaag tgtccaaaat
      541 tgcactggca atgggtctta gcatggcatt ggtaagcggc ttcgcaaacg ctgctgactc
      601 tggtacaact cctcctgcta atactgcggc agaaggcggc catggtcacg gcactgttca
      661 ctttaaaggt gaaatcattg atgcgccatg ctctatttct ccggaatctg cagaccagac
      721 ggttgatctt ggtcaggtcg ctaactctgc cctgaaagac aaaggtacgt cttctccggt
      781 cgctttcaac attaaactgc aaaactgcga catcagcact tacaaaactg taactgcaac
      841 ctggagtggt acacctgacg ttaacatgcc ggaggcatgg ggtatcactg gtacggcgag
      901 cggtgcagct attgttctgc gcgatgcgtc cgaaaaagag atcaccctgg gtcaagacac
      961 tgccccaact acgctgaccg ctgatgacac cactattgct atgtctgcgt tcctgaaagg
     1021 cgacggcgaa gcgattgtcc caggtgcgtt caccggtgcg gcagacttcg ttctttccta
     1081 ccagtaagta gccgcttaac tgtgctggct ccctggttag cagggagccg ttaagaacgt
     1141 gaaatcagac ggaagtatgg tgatgttgcg tattgttttc ctcctgcttt tgctggtgag
     1201 ttcgacaggg cgcgctgttt cacatggtca cgggcatgta caaatgcaag gaaccattat
     1261 tgatacagca tgtgccattg ctacaggaga tgtggagcag gggatagatt tcggcgcact
     1321 tacggcaagt gaattaatta ataatggccg tggtccttct ttgccattta ccgtccattt
     1381 agtgaactgc gttcttgatg ggacagatat tcgaggcctg aatcactgga aagacgtacg
     1441 tataacattt gtaggcgatg ctgatagcga gaaccatttt gctttacagg gagatggacg
     1501 tggcgcggct ctggtcattg ctgatagcca gggggttgag gcgaaacctg gagagcctat
     1561 gccttctaca cccattacac ctgggagtat ggcattgcat tatcaattat ggctaaccgg
     1621 cgggaaaaca atccggcctg ggcatattca taccaccgtt cgctacttca tggagtacga
     1681 ctgatgttta atcagaaatc cggaaataac ggcggattaa ataacgtcag attgaattcg
     1741 ttaacgtgga tgctgttgct cgtattgggt ggtattcctg ctgcgcagtc acaagataac
     1801 atgtcaacgc cagcgaattc gaatattcca gatgaggtag tcgagcagcc gaatataacc
     1861 gagttcaaca ccgatgtgat ggacgtaaac gatcgaaata atatcgacgt tagccatttt
     1921 tcccgtgccg ggtacgttat gcccggggaa tatccgctca tattagttgt gaatggaact
     1981 gaaattcgcg aggtcacggt ttcatttgca ccgccggaag atgacccaca aggcagctcc
     2041 ccgtgtttaa caccggaaat ggtaaagctt tttggtctta aagatgaagc aacgaaaaaa
     2101 ctaatctgga aaggtaacgg tgatgatcgt tgtctggatg aaaagtcact tcccggcatg
     2161 accgctaccg gtgaccttag taccggttca cttaagattt ctttacccca ggcctggctg
     2221 gaatatagcg cgccggactg ggatccgcca tcccgcgggg ataacggagt ggcagactta
     2281 ttatttgatt acaacctgaa cgcatcgaaa agccatgcgt tatctgaagg tggttccagt
     2341 gcgaccagct taagtggtag cggaacggca ggcgcaaatt ttgggccatg gcgtttacgt
     2401 gccgactggc aagcctcgcg ttatagcgac aggggataca cgtcgcagag tatggactgg
     2461 agtcgatttt atctctaccg cgcgcttcct aagcttggcg ccaggttaac tctgggtgaa
     2521 tctgatcttg attcatcact gtttgatagc ttccgcttta tcggcggtag tctggtgact
     2581 gatgacaata tgctgccgcc taatcttcag ggatatgctc ctgaagtgac gggggtagcc
     2641 aggtcaaatg cgaaggtcgt gataagccag caagggcgcg tgatatatga atcgcaggtg
     2701 gcagctggtc cttttcgtat ccaggaatta agcagttccg tcagcggtac cctggatgtc
     2761 cgggtggagg agcaagatgg acgggtacaa cattttcagg ttgatacggc cagtattccc
     2821 tatctgacgc gtccggggtt ggtgagatac aaagcgtcct taggaaagcc tacggactgg
     2881 aaacaccatg tacaggatgt gtcttttgcg acgggagagt tctcttgggg tatgacaaac
     2941 ggttggtcac tatttggtgg cctggtcgca gccaaaaaat atgcggctct tgcagtgggt
     3001 attggtcgcg atttgctgtc atttggtgcg gtttcgtttg atgtcaccga atctaaggcg
     3061 acgcttgata ctggcgaaaa aaaaatgggt ggttcatacc gcctgagtta ttccaaacgc
     3121 tttgagaaaa tagatggaca gataacgttc gcgggctatc gcttttcaga aaaagacttt
     3181 atgtcaatga acgattttct cgaaacagag tatgacgccg gtagcaggag tgatactgcc
     3241 tggggaaggc agaaagagct ttacaccatc acagtcagta aagcttttcc tgactggaat
     3301 atgaatctgt atggcaatta tagccaccgg acctactgga atcgtcccga tgcagatcgc
     3361 tatagcctgt ctttatcgaa atacatggac gttgggcgtt ggaaggggat tagcctgaac
     3421 ctgactggct atcgcagtga ggcatggggt gtgaaggatg atggtgtata tgccagtctg
     3481 agcattccct ggagtgatgg cggttcattg agtttcaacg ggcaaagcac aacggctggc
     3541 acaacacaga tggttggttg gtacgacaga attgatagta acaataatta cagtctgcaa
     3601 gccggtacaa gtgttgggaa taaggcctcg gcgagtggat atgtgtcaca caatggtgat
     3661 ctcacgaatg gaacgctgag tgcgagttat tacgaaggaa acaatacgtc gttagggctg
     3721 tctttgcagg gcggaatgac agtaactcgt tacggagcgg cgttgcatcc tggtgcgatg
     3781 aacggcggta cgcgactgat gattgattct ggtgatgttg ctggtgtgcc gttaagccat
     3841 agtggggttc tgcaacgcac gaatgccttt ggcgtcggcg tcattggtaa ccttaatagc
     3901 tactggcgca acagtgttgg ggtcgatgtt aatgccctgg acgatgatat ggaagtcagc
     3961 catccgttaa cggaattgac catgactgaa agtgcgatcg gttatcgaaa aatcgatgtt
     4021 cttgaaggtc gaaaattgat ggcggtagtg aagtttgcgg atggtagtag tccggcattt
     4081 ggcgccaccg tctggagagg gagccatcag accggcgtcg ttgatgataa cggtagcgtg
     4141 tggttagccg gggttaagcc gggtgtcacg atggaagtcc gtgaagatga aaatccggta
     4201 tgtcaggtag gtattcctga gcgattaccg ccggaaaata ccgatcaatc gctgttatta
     4261 acatgccagc agaaatcata actcattatg tgagtggggc actgtattgg aaaaacagtg
     4321 tgtgtagaga ttaggagaaa aggacgcggc catggacggt acattaatgt tgataattaa
     4381 attcagagaa tcaaatgaac agaaataaat caattaagtc ccagccgttc ctgaaatctc
     4441 ttctggtcat ggctttagtc agtcagtccg catttcaggt tgctgatgcg gcagttgctc
     4501 tggatcgtac tcgggtaatt tttgaaggcg gtaataagag catgagctta aatattcgta
     4561 acgataatac gaagctccct tatttagcgc aatcttgggt agaggatgag caaggaaaca
     4621 aaatacagtc accgctgatg gcattgccgc cgctacaacg tatggagccg ggttcaaaaa
     4681 gcctggtgaa aatacagtta gcaccaggcg ttgaagcaaa attgcctcag gatcgtgaat
     4741 cgttgttcta ttttaattta cgcgaaattc cacctaaaag taataaagcg aacgtcctgc
     4801 aactggcgct acagactcgg gtgaaaatgt tttatcgccc gaaagcgctg gcaaaaaacc
     4861 aggcaatgaa tgaaacaccg tggcaggagc agttgacgct t
//
LOCUS       DADLSB010000233         4832 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.233, whole genome shotgun sequence.
ACCESSION   DADLSB010000233 DADLSB010000000
VERSION     DADLSB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.233"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..979
                     /locus_tag="JN331_003944"
     CDS             <1..979
                     /locus_tag="JN331_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HAY7261920.1"
                     /translation="CGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQL
                     VAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSR
                     ASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPF
                     IGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGL
                     FLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQREAVTETAAALGFISAIGAV
                     GGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1061..4801
                     /locus_tag="JN331_003945"
     CDS             1061..4801
                     /locus_tag="JN331_003945"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7261921.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFQEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
BASE COUNT         1108 a         1312 c         1314 g         1098 t
ORIGIN      
        1 atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt tcttctttcc
       61 aaaagccaaa caagggagtg ctcttgggat taatggcgga ttaggaaact taggtgtaag
      121 tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct ttctcggcgt
      181 caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg catggatttg
      241 ggtaccgcta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg atatcgccag
      301 ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc tctggctgct
      361 gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg gttttgccat
      421 gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct ttggcccatt
      481 tattggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg gcggcgtgcg
      541 ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt tccttacctt
      601 accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg ggctgtttct
      661 gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct ttcgccagat
      721 aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga gagaagccgt
      781 caccgaaacg gcggcagcgc tgggctttat ctcagccatt ggcgcagtgg gcggcttttt
      841 tattccgcag gcatttggca tgtcgctcaa tatgaccggc tctccggtcg gcgcgatgaa
      901 agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt atggtcggcg
      961 gaagttcagc caaaaataag agatcgatgc ctgatgtaca accgtgtcgc atccgacaag
     1021 gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac ttttggatcg
     1081 ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac aggtgatgca
     1141 tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca aaatcgtgcg
     1201 ttccacccac ggcgttaact gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg
     1261 tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg acctgcccaa
     1321 tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt acagcgctaa
     1381 ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg aagcactcaa
     1441 acaacacagc gatccggtac tggcgtgggc atcgattatg aacgatccgc aaaagtgcct
     1501 gagctacaaa caagtgcgtg ggcgcggcgg gttcatccgc tccaactggc aggaactaaa
     1561 ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg atcgcgttgc
     1621 cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa cgcgttatct
     1681 gtcgctgctt ggcggcacct gtttaagttt ctacgactgg tattgcgacc tgccgcccgc
     1741 ctcgccgatg acctggggcg agcaaaccga cgtaccggaa tctgccgact ggtataactc
     1801 cagctacatc atcgcctggg ggtctaacgt accgcagaca cgtacgccgg acgcccactt
     1861 ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc attacccctg actactctga
     1921 agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata gcgccctggc
     1981 gatggcaatg gggcatgtga ttttaaaaga gtttcatctc gataatccca gcgactactt
     2041 tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg agcctcgcga
     2101 cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg atggactggg
     2161 cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat accgcaggtg aattggtagt
     2221 gccgaacggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc tggaatccat
     2281 tgccgccggt acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg acgctgttgc
     2341 aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca gcgtaaaaca
     2401 caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg atggcaacac
     2461 ctgtccggtg gtcagcgttt atgatttggt actggcgaat tacggcctcg atcgcgggct
     2521 ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg
     2581 tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg aatttgccga
     2641 taccgcccat aaaacgcatg ggcgctcaat gattatcctc ggcgcaggtg ttaaccactg
     2701 gtatcacatg gacatgaact accgcgggat gatcaatatg ctgatcttct gcggttgtgt
     2761 cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc gcccacaaac
     2821 cggctggttg ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc agatgaacag
     2881 cacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct ctgctcagga
     2941 gttactttca ccgctcgccg atgccagtaa gtacagcggt catctgattg atttcaacgt
     3001 tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag ctggggcgta acccgctcgg
     3061 gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg cccaggcgct
     3121 gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca atcatccgcg
     3181 taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc acgagtatat
     3241 gcagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg gtgccagcga
     3301 cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca agctcgacct
     3361 gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata tcgttctgcc
     3421 caccgccacc tggtatgaaa aagacgatat gaacacctcg gatatgcatc catttattca
     3481 tccgctttct gcggcggtcg atccggcctg ggaatcacgc agcgactggg aaatctacaa
     3541 aggtattgcc aaagcatttt cgcaagtgtg cgtgggccat cttggcaaag aaaccgacgt
     3601 ggtattacaa ccactgctgc atgactctcc ggcagagctc tcacagccgt gtgaagtgct
     3661 cgactggcgc aaaggcgaat gcgatctgat cccgggtaaa accgcgccga atattgtggc
     3721 ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc cattgatgga
     3781 caaacttggc aatggcggta aagggatttc gtggaatacc caggatgaaa tcgatttcct
     3841 cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc tgattgacac
     3901 cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg gtcatgttgc
     3961 agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc atctggcgct
     4021 gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc gtaaaattat
     4081 ctccagcccc acatggtccg gtctggaaag cgatcacgta tcctataacg cgggatacac
     4141 caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc tctatcagga
     4201 tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc ctatcgacac
     4261 ccgtagcgtc agtgagatgc gccagatacc gccaaacggc ttccaggaaa aagcacttaa
     4321 cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa acctgctaat
     4381 gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaacagatg cccgagaact
     4441 gaccattgtc gataacgact gggtggaagt atttaacgcc aacggcgcgc tgactgcccg
     4501 cgcggtggtc agccaacgtg taccgccggg catgaccatg atgtatcacg cccaggaacg
     4561 cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc ataactcggt
     4621 cacccgcgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc tggcctgggg
     4681 ctttaactac tacggcaccg tcggctcgaa ccgcgacgaa ttcatcatga tccgcaagat
     4741 gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg cgaaaaaatg
     4801 aaaatacgtt cgcaagtcgg catggtgctt aa
//
LOCUS       DADLSB010000234         4820 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.234, whole genome shotgun sequence.
ACCESSION   DADLSB010000234 DADLSB010000000
VERSION     DADLSB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.234"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1103)
                     /locus_tag="JN331_003946"
     CDS             complement(<1..1103)
                     /locus_tag="JN331_003946"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7261922.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMELSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
     gene            complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="JN331_003947"
     CDS             complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="JN331_003947"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY7261923.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3116..4765
                     /gene="treF"
                     /locus_tag="JN331_003948"
     CDS             3116..4765
                     /gene="treF"
                     /locus_tag="JN331_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY7261924.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGKP"
BASE COUNT         1221 a         1245 c         1156 g         1198 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      361 ccaccgctgg cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagcgcat cgtgcagcgg ttgtgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt gttttcgtca caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgtac cggaccgcac actaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatatt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt attgatcgac aactccatca atttatggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggctaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctggaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac
     1021 tccgcgatag tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa
     1081 tccgttaaca gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa
     1141 gtaggattta tccgcaatgg aagcaaggca ttacaggctt aatttaaata acaaaatcct
     1201 aagcagaaaa acgacttttt ataatataaa catttaacat gataatatta aaaacagact
     1261 ttattatcgt aataaaaatc gcccaaacat tcctgttcgg gcgatcaaat taattattgt
     1321 ttatcctgca tatacggcgt gtacaccccg ttcagactgt gcaggaaagc tacgatatca
     1381 tccacatcct cctgcggcag ctctttgcct acctgatagc gcagcatcag tttcaccgcc
     1441 ccgtccagcg tcggcacgtc accgcgatgg aagtacggtg ccgttaaagc aacgttacgt
     1501 aaaccgggta ctttctgacg caatttatca cgctcttctt tagtcacatt catacgacca
     1561 atatccgccg ccgtaatttc cccaaagtta aagtcttttt tcagccccaa cggttcaaag
     1621 gaacgtccgc cgagaataat accaccatga caagttgcac atttattatc tttaaataat
     1681 tgatagcctt ttttctgttg cgccgtcagc gcattttcat ctccacgcaa ccatttatca
     1741 aatggggaat ccggcgtaat taatgttttc tcaaattcag caatggcatc agtaatattt
     1801 tcgccactga aaccttgcgg ataaacttcg aggaactgcg ctttaagctg cggatctttt
     1861 tccagcttag caataatttc gtcccaggat ttcgacgcca tttcaatcgg gttcaacggc
     1921 ggtccacctg cctgatcctg caatgttgcc gcacgaccat cccagaactg ctcaacgtta
     1981 aatactgagt taaataccgt cggcgcatta atcggcccaa ctgcgccacc aacaccaatc
     2041 gatgtttttc tgccatcgac gccccccgca ttcaacgcat ggcaatgagc gcatgaaatg
     2101 gtgctatcag ccgataaacg gggatcgtga tacagcgcaa agcccaacgc cactttttgc
     2161 gcatcggtag gcagtttttg cgggatgggc tgcaccggtt cattgcgatg ctccggagca
     2221 gtatcattgc tggcgtaata ttccgcgcgc tgttttgcaa tccaggccag tatttccgcc
     2281 cgctcttcat cactcacctt acccgcccag tgtagcgcgg tataacgcgt tggtggcata
     2341 gtttcatact gcatcaccca ttcaatctta ttcagatcgc tttgcgaaac gggtttatca
     2401 gccagcagtg ccgcacgcac ggcctcgagg ttaaaagatt tatatccaag cttaatgtcg
     2461 taatccatca actgtttcgc gccaggaata taataatagg cgggtaattc tgccgaaggc
     2521 gtgtggcaat agtcgcatcc tttttcgcgg agaaagccta aaaccttatt attttcactg
     2581 acagcagatg cctgaacatc ggcctgttta ctgcgtttat tatcgtggta ccacacataa
     2641 ccagataacc ctaaatagca aatcgcgacg ccagccaggc cgatcgcggt aatacgtgag
     2701 accattttca ttattcttcc ctcacggtgg tgagttattg tgacccagaa aattttccag
     2761 aatgctgcaa acgtctctgc gagacaaagc catcataaaa aacagggaga tacgcttttt
     2821 gataggaatc aatcaattac atgaatataa actatcaata agatagcctg tgcctattac
     2881 ccagatcagg aatggcatat catgattgcg gcgagagcag gattgaatgt tctgaaaatg
     2941 aaaaacaaca ccaaatccgt atttttataa attccattga tattagtgcg ttatattttt
     3001 tgacaagtta ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga
     3061 ctacccttaa agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct
     3121 caatcagaaa attcaaaacc ctaatccaga cgaactgatg atcgaggtcg atctctgcta
     3181 tgaactggac ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga
     3241 gatgattgaa gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact
     3301 gttcgagcat gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc
     3361 acccaaaatg gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga
     3421 ttttgacttg cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga
     3481 gtatgtatcg gacccgcaaa attccctgaa agagcatatc gaccagctgt ggccggtgct
     3541 aacccgcgaa ccacaggatc acattccgtg gtcttctctg ctggcgctac cgcagtcata
     3601 tattgtcccg ggcggccgtt ttagcgaaac ctactattgg gattcctatt tcaccatgct
     3661 gggactggcg gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg
     3721 gatgatcgaa aactacggtc acatccccaa cggcaaccgc acctattatt tgagccgctc
     3781 gcaaccaccg gtttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc
     3841 gcgccgctat ctcgaccacc ttaaaatgga atatgccttc tggatggacg gtgcagaatc
     3901 gttgatccct aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa
     3961 ccgttactgg gacgatcgcg acacgccacg tgacgaatcc tggcttgagg acgttgaaac
     4021 cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggagcggc
     4081 ctccggttgg gattactctt cccgttggct gcgtgatact ggtcgtctgg cgagcattcg
     4141 taccacccag ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat
     4201 cgccaacatc tcggcgctga aaggcgagaa agagacagaa gcgctgttcc gccagaaggc
     4261 cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg
     4321 cgattacgac tggcgacgcg aacaactggc gctgttttcc gctgccgcca ttgtgccgct
     4381 ctatgtcggc atggcgaacc atgaacaggc cgatcgtctg gcaaacgccg tacgcagccg
     4441 gttactgaca cctggcggga ttttggcaag cgagtacgaa accggtgaac agtgggataa
     4501 acccaacggc tgggcaccgt tacaatggat ggcaattcag ggatttaaaa tgtatggcga
     4561 tgaccttctg ggtgatgaaa tcgcgcgcag ctggctgaaa acggtgaatc agttctatct
     4621 ggaacagcac aaaatgatcg agaaatacca tattgccgat ggtgttcccc gcgaaggcgg
     4681 cggtggcgag tatccgttgc aggatgggtt tggctggact aacggtgtgg tacgccgttt
     4741 aattggtttg tacggcaaac cataatattt ttacagccag ccgctaactt cctgctggct
     4801 gtaaaattat cctcttcagg
//
LOCUS       DADLSB010000235         4775 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.235, whole genome shotgun sequence.
ACCESSION   DADLSB010000235 DADLSB010000000
VERSION     DADLSB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.235"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..235
                     /locus_tag="JN331_003949"
     CDS             <1..235
                     /locus_tag="JN331_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7261925.1"
                     /translation="VIADHDKPQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKV
                     MGGGEELSNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            377..598
                     /locus_tag="JN331_003950"
                     /pseudo
     CDS             377..598
                     /locus_tag="JN331_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(619..681)
                     /locus_tag="JN331_003951"
                     /pseudo
     CDS             complement(619..681)
                     /locus_tag="JN331_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1132..2544
                     /gene="ospC1"
                     /locus_tag="JN331_003952"
     CDS             1132..2544
                     /gene="ospC1"
                     /locus_tag="JN331_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY7261926.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2648..2797)
                     /locus_tag="JN331_003953"
                     /pseudo
     CDS             complement(2648..2797)
                     /locus_tag="JN331_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2873..4570
                     /gene="ospD3"
                     /locus_tag="JN331_003954"
     CDS             2873..4570
                     /gene="ospD3"
                     /locus_tag="JN331_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY7261927.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFAENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4687..>4775
                     /locus_tag="JN331_003955"
     CDS             4687..>4775
                     /locus_tag="JN331_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7261928.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGV"
BASE COUNT         1654 a          868 c          895 g         1358 t
ORIGIN      
        1 ggtaattgca gaccatgaca aaccgcagcc gtcaggctgc ctactcgagt cacaaggctc
       61 tctgtgtccg atatgccggc agcgcattac ccatgaaact ggctggaacg tacaccataa
      121 ggtgaagaaa gttatggggg gcggtgaaga attatctaac cttgtcttgt tacatccaaa
      181 ttgccatcga cagttacaca gttgtgaagc cggctctctc aaaaagggct tataaaggct
      241 tgagggtgta ccgggaaacc ggtatgcacc gttcttaggg gagggcagtc cagcaatgaa
      301 ctgtccttac ccgacaaaca tccaaaacga acggaacgtg ctgaaatccg catataaccc
      361 gttgtaaagt gaggaaatgg acacctcact tgctcatgag aacgcccgcc tgcgggcact
      421 gttgcagacg caacaggaca ctatccacca gatggctgaa tacaaccgcc tgctctcaca
      481 gcggatggcg gcttatgctt ccgaaatcaa ccgactgaag gcgctggttg cgaaactgca
      541 gcgtatgcag ttcgataaaa gctcagaaaa acttcgcgca aaaaccgaac ggcagattcc
      601 attttcacgg gcgatctacg ccacacaggc tctgggttgc agtgactgct ccattaaggc
      661 gattttgaac tcataaggga agtaacaaaa aaactgcaaa agtacaataa ttatatatgg
      721 ccaaactaac ctctattaaa aaaacatcag tttttcacac ttagttgagc ttcatgaaaa
      781 aatcatcgag ggtttcattg cagaatacac attccactaa ctggaagcac tccataagag
      841 gaatcagaga aagtatcgtt tttttatagt aaaattcatt gctgttcaat aaaattgata
      901 tagttattaa attatgaaaa taatttcaca aaaaagatta acttgagttc ttaagcatcg
      961 gattttagat attttgaaat atctggcatc acataacatc aaaagtatat aataccctta
     1021 tctaattaca tgcagtatat tgtatatata ctcaaaaatt gtaattcata ccctgctata
     1081 tcttgtaggg aaatatatta attaaaactg ttttcatata aggttcattt tatgaatata
     1141 tcagaaacac tgaactcagc aaatacccaa tgcaatatag attctatgga taacagatta
     1201 catacattgt ttccaaaagt gacatcagtg cgaaacgctg cacaacaaac tatgccagat
     1261 gaaaaaaatt taaaagatag tgcaaatatt attaaagatt tctttaggaa aactatagca
     1321 gcacagagtt atagtagaat gttctctcaa ggctctaact ttaaatcttt aaatatagca
     1381 attgatgcac catcagacgc taaagcctca tttaaggcta ttgagcacct tgacagatta
     1441 tcgaagcatt atatatctga aataagggaa aaacttcatc ctctttctgc agaggaactc
     1501 aatttgcttt cgctaattat taattctgat ttaatcttca gacatcaaag taattctgat
     1561 ttgtctgata aaattttaaa cattaagtca ttcaataaaa ttcagtctga aggaatatgc
     1621 acaaaacgaa acacatacgc tgatgatata aaaaaaatag ctaatcatga ctttgtgttt
     1681 tttggcgttg aaatctctag ccatcagaaa aaacaccccc tgaatacaaa acatcacact
     1741 gttgattttg gtgcaaatgc gtatatcatt gatcatgact ctccatatgg atatatgaca
     1801 ttaaccgatc actttgataa tgctattcca cctgtttttt accatgagca ccaatcattt
     1861 ttagataaat tttcagaggt taataaagaa gttagtcgat acgtacatgg aagtaaagga
     1921 attatagatg taccaatatt caatactaaa gatatgaagt tagggctcgg attatacctg
     1981 attgacttta ttagaaaaag tgaagaccaa agcttcaagg agttttgcta tggaaaaaat
     2041 cttgcccctg tggatctgga tagaatcata aactttgttt ttcagccaga gtaccatata
     2101 cctaggatgg taagtacaga aaacttcaaa aaagttaaga ttagagaaat atccttagag
     2161 gaggctgtta cagcatctaa ttacgaagaa attaacaagc aggtcactaa caaaaaaatt
     2221 gctctccagg ctctttttct ttcgattact aatcaaaaag aggatgtcgc cttatatata
     2281 ttatctaatt ttgagataac tagacaagat gttatttcca taaagcatga gttgtatgat
     2341 attgagtatc tacttagcgc tcataattca agctgtaaag tacttgagta ttttatcaat
     2401 aagggattgg ttgatgtaaa cacaaagttc aaaaaaacta atagtgggga ttgtatgttg
     2461 gataacgcaa taaaatatga gaatgcagaa atgataaaac tattattgaa atatggtgca
     2521 acatctgaca ataaatatat ttaatcaaaa ttgaatatcg tttagcaaaa ggcagtattt
     2581 gcacaaaata tcgatatatt gtttattgtc agtatggctc aatgtgataa tagttggaaa
     2641 gtttgatggg tttcgccccg ttgtagcggt agtcgagctg gaaggtcttc aggcactgct
     2701 tacagcgata gagcagcccc cctgaactgg aatggccgtt ccggataccc ccctgagttt
     2761 cagagtaacg gggacaaacc acatcaatct ttgccatcaa tcatccaaag ggcaaagagt
     2821 acaacaacac taagtctgcg tcacaaccca tcaatgaaag gaatatatac atatgccatc
     2881 agtaaattta atcccatcaa ggaaaatatg tttgcaaaat atgataaata aagacaacgt
     2941 ctctgttgag acaatccagt ctctattgca ctcaaaacaa ttgccatatt tttctgacaa
     3001 gaggagtttt ttattaaatc taaattgcca agttaccgat cactctggaa gacttattgt
     3061 ctgtcgacat ttagcttcct actggatagc acagtttaac aaaagtagtg gtcacgtgga
     3121 ttatcatcac tttgcttttc cagatgaaat taaaaattat gtttcagtga gtgaagaaga
     3181 aaaggctatt aatgtgcctg ctattattta ttttgctgaa aacggttcat ggggagatat
     3241 tattttttat attttcaatg aaatgatttt tcattccgaa aaaagcagag cactagaaat
     3301 aagtacatca aatcacaata tggcattagg cttgaagatt aaagaaacta aaaatggggg
     3361 ggattttgtc attcagcttt atgatcccaa ccatacagca actcatttac gagcagagtt
     3421 taacaaattt aacttagcta aaataaaaaa actgactgta gataattttc ttgatgaaaa
     3481 acatcagaaa tgttatggtc ttatatccga cggtatgtct atatttgtgg acagacatac
     3541 tccaacaagc atgtcctcca taatcagatg gcctaataat ttacttcacc ccaaagttat
     3601 ttatcacgcg atgcgtatgg gattgactga gctaatccaa aaagtaacaa gagtcgtaca
     3661 actatctgac ctttcagaca atacgttaga attacttttg gcagccaaaa atgacgatgg
     3721 tttttcagga ttgcttttag ctttacaaaa tgggcattca gatacaatct tagcatacgg
     3781 agaactcctg gaaacttctg gacttaacct tgataaaacg gtagaactac taactgcgga
     3841 aggaatggga ggacgaatat cgggtttatc ccaagcactt caaaatgggc atgcagaaac
     3901 tatcaaaaca tacggaaggc ttctcaagaa gagagcaata aatatcgaat acaataagct
     3961 gaaaaatttg ctgaccgctt attattatga tgaagtacac agacagatac ccggactaat
     4021 gtttgctctt caaaatggac atgcagatgc tatacgcgca tacggtgagc tcattcttag
     4081 cccccctctc ctcaactcag aggatattgt aaatttgctg gcctcaagga gatatgacaa
     4141 tgttcccgga cttctgttag cattgaataa tggacaggct gatgcaatct tagcttatgg
     4201 tgatatcttg aatgaggcaa aacttaactt ggataaaaaa gcagagctgt tagaagcgaa
     4261 agattctaat ggtttatctg gattgtttgt agccttgcat aatggatgtg tagaaacaat
     4321 tattgcttat gggaaaatac ttcacactgc agaccttact ccacatcagg catcaaaatt
     4381 actggcagca gaaggcccaa atggggtatc tggattaatt atagcttttc aaaataggaa
     4441 ttttgaggca ataaaaactt atatgggaat aataaaaaat gaaaatatta cacctgaaga
     4501 aatagcagaa cacttggaca aaaaaaatgg aagtgatttt ctagaaatta tgaagaatat
     4561 aaaaagctga atattatctc gtggtgatac actgaatttg aggtaaccgg ctcatttaaa
     4621 ccgtctggtc tgttacctcc ggttccacaa aaataatgtc catcattttg aagtggacac
     4681 tatcgtatgg aataccggac ctggatcact gaagctttac gccttcattt cgaagaacat
     4741 ttaccacggg ttgtggccgg acgtcgcctg ggcgt
//
LOCUS       DADLSB010000236         4697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.236, whole genome shotgun sequence.
ACCESSION   DADLSB010000236 DADLSB010000000
VERSION     DADLSB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.236"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            229..1251
                     /locus_tag="JN331_003956"
     CDS             229..1251
                     /locus_tag="JN331_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY7261929.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1251..2231
                     /locus_tag="JN331_003957"
     CDS             1251..2231
                     /locus_tag="JN331_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY7261930.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2228..2986
                     /locus_tag="JN331_003958"
                     /pseudo
     CDS             2228..2986
                     /locus_tag="JN331_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2996..3805
                     /locus_tag="JN331_003959"
     CDS             2996..3805
                     /locus_tag="JN331_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY7261931.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            3805..4659
                     /locus_tag="JN331_003960"
     CDS             3805..4659
                     /locus_tag="JN331_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY7261932.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
BASE COUNT         1060 a         1280 c         1183 g         1174 t
ORIGIN      
        1 gtggacgata aaaaacaata atgtcattca tatttaaatg atttagcctt aaatgattta
       61 aataatatta gccacctgat aaatacattt ttaatgatag agagggtcaa caggcttgcg
      121 gaagtcgtca catcttcatg ttttcaatag ctaaactttg atattgaaca tgatttttat
      181 cgatatatat gaaagtatat atcgattttg cttttcagga tgttcaccat gcccataacc
      241 agacggacgt ttgcgcaggc acttgcatct accctgctcc tgcagtctct accgtccttt
      301 tcacaaacag ttaatcgttt tgcttcgcaa tctcttcccg aagcccaaaa cattacacgc
      361 atcgtcagcg caggcgcgcc cgccgatttg ctattgctgg ctgtcgcgcc agaaaaaatg
      421 gtcggttttt cctcttttga ttttgcccgt caggcattaa ttccattgcc agagcacatt
      481 cgccagttac ccaggctggg acgactcgcc gggcgcgcca gcacactatc gctggaaggg
      541 cttatggcgt tacatcccga tttggtcgtt gattgcggca atacggatga aaccttgatc
      601 tcccaggcac ggcaggttag cgaacagaca caaatcccct ggttattgct taacggaaaa
      661 ctggcgcaat cagcagaaca gttaacaacg cttggcaaaa cgttaggcga agagcaccgc
      721 gccgcagaac aagccaatct cgccagccac ttcgttggtg aagctcaggc attcgccacc
      781 tcacccgccg ctaacctccg cttttatgct gcgcgcggtc cccgagggct ggaaacgggc
      841 ttacagggtt cgttgcatac cgaggcggcg gaattattag gtttgcacaa cgtcgcgcaa
      901 atagccgatc gccacggcct gacacaggtt tccatggaaa atctcctgcg ctggcagccg
      961 gatattattc tggttcagga ggccgttact gcagatttta ttcgtcgtga tcctctctgg
     1021 cagggcgtga aagccgttgc ggaacaacgc atcttatttt taagtggctt gccctttggc
     1081 tggctcgatg ccccgccggg aatcaaccgt cttctgggat tacgcagact tcacgcctgg
     1141 ctggatcccg ccatcaatcg ccagtttaaa agtgacatgc agcattacgc ccaactgttc
     1201 tggcattgtt cactcagtga tgccgactat caaaaattgg tggcgagcta atgagaatcg
     1261 taaacggatg tattttgctg ctggcagcta tcagtatcac ttttgccgcc gtaagtgggg
     1321 cttatcatct cgatatgcag caactgctgg cgctaattct tcgtcaagaa aatgtgtctg
     1381 tgcaggaaca aattgtcttc tggcaaatcc gcgtgccgcg tattcttgct gcgctgttac
     1441 tgggcgcagc gctggcagga gccggaacca cctatcaggg aatgttgcgt aatcccttag
     1501 tttcaccgga tattttaggc gtctccgccg gtgccggact tggtgcctgc gctgcaatcc
     1561 tgtggggatt acccattgtt tacatccagc tgtacgcatt ttgcggcggg ctgatggtgg
     1621 tcgcgggcgt ctggttgatt acgcgtcggg tgacccgcca cgatccgatc ttaaccctgg
     1681 tgctggtagg cattgcgtta ggaacgttgt gcggagcggg gatatcgcta attaaaacgc
     1741 tggccgatcc ttacacccag ttaccgtcaa ttaccttctg gttgctgggg ggactgtcca
     1801 ccgtgacctt acgcgatctc tgctatgctg caccgatcat tctcataggc tccttgcccc
     1861 tgttttttct ccgctggcgc atgaatttac tcacgctttc cgacgacgaa gcccgttcgc
     1921 tgggactgaa cgtgacgcgt ctgcgctttg gattgattgt ctgcgccaca ctaatcaccg
     1981 ccagcacggt agcaatagcg ggcataattg gttggatcgg tctggtcgtt ccgcacattg
     2041 cccgcttgtt gaccggtcat aaccatcagc agctgttgcc tatggcaatg tgtactggcg
     2101 ctattctgct cctgcttacc gatacgctgg cgcgcagcat tggaacaaca gaaatcccac
     2161 ttggcattct gaccgctttt gttggcgcac cgtttttcct gcttttgttg ttacgcggag
     2221 gtcgccagtg actttgcttg ccgttcgtca tgcgtctctg ggttactccc gccatccagt
     2281 gctacgcgac gtgtccttca ccctgtcgca aggaacaatt tgttgtctgc ttggcgcgaa
     2341 tggttgcggc aaaaccacac tcatgcgctc cattcttggc gttataccgt tattaaaagg
     2401 cgagatccta ctggatagcc tccctgtgca gacactcagc catcgccagc gcgcacaggc
     2461 tattgcctag gtgccgcagg cgcacgacgg tatttttgct ttcagtgtgc tggatatggt
     2521 gttgatgggc ctggccccca ccataggcgc tttttccgtt cccggtaagc aagaacgcct
     2581 gaaagccacg gagcaactgg aaaagctggg cattttgcat ctggcgacac gacgctggaa
     2641 tacgcttagc ggcggtgaac gtcagctggc tttaatcgcc cgtgcactcg tccaacaacc
     2701 gcgcttgttg ttactggatg agcccgcctc cagcctcgat ttcggtcatc agatccaact
     2761 tctggacacg ctggcgcagc tcaaaaataa cggcatgaca atgttaatgt cgactcacca
     2821 tccgctccat gccaacgcca ttgccgacag cattatccag gtagagccag atggacgagt
     2881 aacacaaggc ttaccgaccg aacagttaac aacgaacaaa ctggcggccc tgtatcgggt
     2941 ttctgccgac caaattcatc atcacctctc tgcaattagc cgctaaggaa caatcatgct
     3001 cattgatgat attgattttg cagaccttta tctgcagcaa ctcaaactgg ctcaccgcac
     3061 agaaaaaacg cccgatcact gggatcaacg ggcggagaaa atggcggaaa attgcgcctc
     3121 gccaacggac agctacctgc aacaactgat cgctaaaatt gacctgcaag gcgctcagac
     3181 cttattcgat atgggctgcg gaccaggcac cgtatcgctg gcgctggctg acaaactcac
     3241 tacggtttat ggcgttgatt acagtcaggg gatgctgaac gttgccgccc gccgcgccgc
     3301 cgcactgaag gctgacaatg tccactggat ccagcgcgcc tgggaagagg actggagtga
     3361 cctgccacgc tgcgatattg ccgtggcttc ccgctccact ctggtggctg atatgcgcca
     3421 tgcaatgagc aaactgaata atcaggcacg gcttcgcgtc tatacaactc atctggtcag
     3481 cacctcgttc gtctctccag ccattcagcg ggcggctggt cgggaagtga tcgaattacc
     3541 caactatatc tttgcgctca acgtcctgta tcagatggga atttatgccc acgttgattt
     3601 tatccgtgga caaaattgcc aacaggataa cagcacctgg gaacgttttg agcagaacgt
     3661 cagctggagt cttggcgcgc ttaacgacga tgagcgtgag cggttatatc actggtatca
     3721 acaacaagat gctcgtgccc tcgcccctgc cagccgcgac tgggcgctca tctggtggga
     3781 tagcgtaccg caggaggcac tgtgatgatt ttcctttctc aggcacaaat cgatgcgtta
     3841 ctgctggaag atatccaggg aggtgacctg accacgcgag cattaaatat tggacaccag
     3901 cctggctata tagcgttttt tcaccgtcag ggcggctgcg tcagcggcgt ttctgtcgcg
     3961 tgtaagatgt taactacgct gggattaact attgatgacg cggtcagcga cggttcacaa
     4021 gcgaacgcgg gtcaacggct aatccgtgcg cagggtaatg ccgcagcact tcatcaggga
     4081 tggaaggcag tccagaatgt gctggagtgg agttgcggtg tttctgatta tctcgctcaa
     4141 atgctggcat tgcttcgtga acgttatcct gacggcaata tcgcctgcac ccgaaaagca
     4201 attccgggca cccgcttact ggcttcgcag gcaattctgg ctgccggagg actaattcat
     4261 cgtgccggat gtgcggaaac catattattg tttgccaacc accgccattt tcttcatgac
     4321 aatcagggct ggtcaggcgc aatcgctcag ttacgtcgcc acgcacccga gaagaaaatt
     4381 gttgttgaag ccgacacgcc gaaagaggct atcgccgcgt tacgcgcaca accagacgta
     4441 cttcagctcg acaaatttag tccgcaacag gcaactgaaa ttgcgcaaat agcgccgtcg
     4501 ctggctcctc actgcacgct ggctctgacc ggcggaatta atctgacgac actcaaaaat
     4561 tacctcgact gcggcattcg cctttttatt acctccgcgc cttattacgc ggcaccagct
     4621 gacattaaag tcagtctgca acccacagcc tctatttaat tctttgttaa acggaattta
     4681 ttctatgaga ctcaaaa
//
LOCUS       DADLSB010000237         4679 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.237, whole genome shotgun sequence.
ACCESSION   DADLSB010000237 DADLSB010000000
VERSION     DADLSB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.237"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            120..3014
                     /locus_tag="JN331_003961"
     CDS             120..3014
                     /locus_tag="JN331_003961"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7261933.1"
                     /translation="MVVLLTPGDDPLRAFLIWVLSESIGALALVPLGLLFKPHYLLRH
                     RNPRLLFESLLTLAITLTLSWLSMLYLPWPFTFIIVLLMWSAVRLPRMEAFLIFLTTV
                     MMVSLMMAADPSLLATPRTYLMSHMPWLPFLLILLPANIMTMVMYAFRAERKHISESE
                     TRFRNAMEYSAIGMALVGTEGQWLQSNKALCQFLGYSQEELRGLTFQQLTWPEDLNKD
                     LQQVEKLISGEINTYSMEKRYYNRNGDVVWALLAVSLVRHTDGTPLYFIAQIEDINEL
                     KRTEQVNQQLMERITLANEAGGIGIWEWELKPNIFSWDKRMFELYEIPPHIKPNWQVW
                     YECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIAVKDGIRHIRALANRVLNKEGEVERL
                     LGINMDMTEVKQLNEALFQEKERLHITLDSIGEAVVCIDMAMKITFMNPVAEKMSGWT
                     QEEALGVPLLTVLHITFGDNGPLMENIYSADTSRSAIEQDVVLHCRNGGSYDVHYSIT
                     PLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSASHDALTHLANRASFEKQLRILLQTV
                     NSTHQRHALVFIDLDRFKAVNDSAGHAAGDALLRELASLMLSMLRSSDVLARLGGDEF
                     GLLLPDCNIESARFIATRIISAVNDYHFIWEGRVHRVGASAGITLIDDNNHQAAEVMS
                     QADIACYASKNGGRGRVTVYEPQQAAAHSERAAMSLDEQWRMIKENQLMMIAHGVASP
                     RIPEARNLWLISLKLWSCEGEIIDEQKFRRSFSDPALSHALDRRVFHEFFQQAAKAVA
                     SKGISIALPLSVAGLSSATLVNDLLEQLENSPLPPRLLHLIIPAEAILDHAESVQKLR
                     LAGCRIVLSQVGRDLQIFNSLKANMADYLLLDGELCANVQGNLMDEMLITIIQGHAQR
                     LGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIADAQPLDLLVNSSYFAIN"
     gene            complement(3124..3972)
                     /gene="alkA"
                     /locus_tag="JN331_003962"
     CDS             complement(3124..3972)
                     /gene="alkA"
                     /locus_tag="JN331_003962"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY7261934.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            4106..>4679
                     /gene="yegD"
                     /locus_tag="JN331_003963"
     CDS             4106..>4679
                     /gene="yegD"
                     /locus_tag="JN331_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7261935.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQLPEAITQAVIGRPINFQG
                     LGGDEANAQAQGILERAAKRAGFKDVVFQYE"
BASE COUNT         1090 a         1223 c         1265 g         1101 t
ORIGIN      
        1 cagcgcccca ccagacccgg tgacatcacc ccccggatca tggacgactg ggggcagtaa
       61 tcatgcctgg tctggctggc taacacccca ttataagggg tacgaataaa gtaacggggc
      121 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
      181 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      241 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      301 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      361 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      421 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      481 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      541 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      601 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      661 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      721 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      781 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      841 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      901 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      961 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
     1021 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
     1081 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1141 aagatcgcca gcatgccgaa aaagtgattc gtgattcgtt gcaatcacgc tcgcccttta
     1201 agctggaatt tcgcattgcc gtgaaagatg gcatccgcca tatccgcgcc ctcgccaacc
     1261 gggtactgaa taaagaaggc gaagtcgaac gcctgctcgg cattaatatg gatatgaccg
     1321 aggttaaaca gcttaacgag gcattgtttc aggaaaaaga gcgcctgcac attacgctgg
     1381 attccatcgg tgaagccgtg gtctgtattg atatggcgat gaaaattacc tttatgaatc
     1441 cagtggcgga gaagatgagc ggctggacgc aggaagaagc attaggtgtt ccgctcctga
     1501 cggtgttgca tattactttt ggcgacaacg gaccattaat ggagaacatt tacagtgccg
     1561 acacctcacg ttccgcgatt gaacaagatg tggtgttgca ctgccggaac ggcggcagtt
     1621 acgacgtgca ttacagtatt acgccgttaa gtactctgga cggcagcaat ataggctcgg
     1681 tactggtgat tcaggacgtc accgaatcac gcaaaatgct gcgccagctg agctacagcg
     1741 cctcccatga tgcactgacg catctcgcca atcgcgccag ttttgagaag caactgcgta
     1801 tcctgctgca aacggtaaac agtacacatc agcgacatgc cctggtgttt atcgatcttg
     1861 atcgctttaa agcggtgaat gacagcgccg ggcatgcggc gggtgacgct ttactgcgcg
     1921 aactggcgtc gttgatgctg agtatgctgc gctccagcga cgtgctggcg cgactcggtg
     1981 gtgatgaatt tggtctactg ttgccagact gtaatatcga aagtgcgcgt tttatcgcta
     2041 cacgcattat cagtgccgtg aatgattatc actttatatg ggaaggccgt gtgcatcggg
     2101 taggtgccag tgccgggatt accttgattg atgacaacaa tcatcaggcg gcagaagtga
     2161 tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg tggacggggc cgggtgacgg
     2221 tttacgaacc acagcaagct gccgcacata gcgaacgggc ggcgatgtcg cttgatgaac
     2281 agtggcggat gattaaagag aatcagttga tgatgatcgc ccacggtgtc gcttcgccgc
     2341 ggatcccgga agcgcgtaat ttgtggctga tttcacttaa gctctggagt tgcgaaggcg
     2401 agattattga tgaacaaaaa tttcgtcgta gcttcagcga tccggcactt agccatgctc
     2461 ttgaccgacg ggtattccac gaatttttcc agcaggccgc aaaagcggtt gccagtaaag
     2521 gcataagcat cgccctcccc ctttccgttg ccggtttgag tagcgccacg ctggtgaatg
     2581 atctgcttga gcagctggaa aatagccctc taccaccacg gttattacat ctgattattc
     2641 cggctgaagc gattttagat cacgcagaaa gcgtgcaaaa actgcggctg gcgggatgtc
     2701 ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt caactcgctg aaagcgaata
     2761 tggcagatta cctgctactt gatggtgagt tatgcgccaa cgtgcagggt aatttgatgg
     2821 atgagatgct gattacgatt attcaggggc acgctcagcg actcgggatg aaaaccatcg
     2881 ccgggccagt cgttttaccc ttagtgatgg atacgctttc tggcatcggc gtcgatctga
     2941 tttatggtga tgtgattgcc gatgcccaac cgctggattt gctggtgaat agcagttatt
     3001 tcgcgattaa ctgagagagc gtttgcccat tgcctgatgc gatgctaacg cgtcttatca
     3061 ggcctacaaa tcgctcattc cgcaggcggg ataaggcgct cgcaccgctt ccggcgacca
     3121 acgtcatgct tcgtctggtt gccagccttc cgtataccag atatgcaaca gcgcataaga
     3181 acgccagggc ttccagcgct cggcataacg gcggatttgt gccggtgtca tccccagaaa
     3241 tcgctgttta atcagataat catccggcag aaaaacatct ttcgcctgcc agccgcgcaa
     3301 ggcgaaataa ttcgccgtcc agcgcccgat acccggaaaa gtttgcagcg ttttcatcgc
     3361 ctgctccaca tcgcccggta ttgtcattgg taaggtgccc tccagcgccg catttgccag
     3421 atgaatcagc gcctctgccc gtttcaacgg catacctaac gcttttaatg cctgcgggtc
     3481 ggctgctgcc agccgctgag gcgtggggaa gcagatatac tccggaaaat catccagccg
     3541 ttcgccataa agctgtgcca ctctggcggt caattttgcc gccatcgcca cgctcaccag
     3601 ttggcctaaa atcgcccgca cgccctgctc aaaagcatca acactgccgg gtaaacgcaa
     3661 tccgggccgc gccgcgccta acttgcccaa cgcaccgtta acaatctgcg ggttacattg
     3721 cagatcaagc aggcggctca ttttcgccag acactcagcg gcaacgggtt ctaaacctgc
     3781 acttaaattt atgtgcagag tatggcgggc tatatccgga atagcagtca ccacgccgcg
     3841 atattcgccc actgccagac tacgggcata ataactgtcc gcgaccgttt ccacaccact
     3901 caccgcacgg gcggcgagaa atcccaacat ccacgaccag tcatacggcg gctgccagtt
     3961 caggatatac atcgcatctc cttattcatc cgctttcagc ataaacgtta ttcagacgtt
     4021 acgctttgct ttcatattcc ggttgtcgcg acggcaacat ttcgctaaag tcacgcccct
     4081 tcttcaccgg catggggatt atttcgtgtt tattggtttt gattacggta cagcaaactg
     4141 ttcagtggcg gtcatgcgtg acggtaaacc gcatttgcta aaaatggaaa acgacagcac
     4201 gctgctgcct tcaatgcttt gcgcgccaac gcgtgaagcg gtaagcgaat ggctgtaccg
     4261 ccatcatgac gttccggcag acgacgatga aacgcaggcg ttgttacgac gggcgattcg
     4321 ttataaccgc gaagaagata tcgatgttac agcgaaaagc gtgcagtttg gtctttcctc
     4381 actggcacag tacattgatg atccagaaga agtgtggttt gtgaaatcac caaaatcgtt
     4441 cctcggtgcc agcggcttaa aaccgcagca ggtagcgctg tttgaggatc tggtctgcgc
     4501 aatgatgttg cacattcgcc agcaggcgca gctgccagaa gcgattactc aggcggtgat
     4561 tggtcgcccg atcaacttcc aggggctggg cggcgatgaa gcaaacgccc aggcgcaggg
     4621 gattctggaa cgcgcggcga agcgtgccgg gtttaaggat gtggtattcc agtacgagc
//
LOCUS       DADLSB010000238         4633 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.238, whole genome shotgun sequence.
ACCESSION   DADLSB010000238 DADLSB010000000
VERSION     DADLSB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.238"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(1..1127)
                     /gene="traD"
                     /locus_tag="JN331_003964"
                     /pseudo
     CDS             complement(1..1127)
                     /gene="traD"
                     /locus_tag="JN331_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
     gene            complement(1555..1746)
                     /locus_tag="JN331_003965"
     CDS             complement(1555..1746)
                     /locus_tag="JN331_003965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261936.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            complement(1743..2165)
                     /locus_tag="JN331_003966"
     CDS             complement(1743..2165)
                     /locus_tag="JN331_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY7261937.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSEFVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMEVVLQALDGEHMS"
     gene            complement(2212..2637)
                     /locus_tag="JN331_003967"
     CDS             complement(2212..2637)
                     /locus_tag="JN331_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY7261938.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIVFHPECNAIYAALD"
     gene            complement(2610..3044)
                     /locus_tag="JN331_003968"
                     /pseudo
     CDS             complement(2610..3044)
                     /locus_tag="JN331_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052648.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
     gene            complement(3215..3997)
                     /locus_tag="JN331_003969"
                     /pseudo
     CDS             complement(3215..3997)
                     /locus_tag="JN331_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            4170..4633
                     /locus_tag="JN331_003970"
                     /pseudo
     CDS             4170..4633
                     /locus_tag="JN331_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154977.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1076 a         1308 c         1247 g         1002 t
ORIGIN      
        1 tgagtcacgt cttctccgga aacaacctcc gggacatccg gttcgaagag gctggccatc
       61 tgacgacctt ctgcttccct tgcggcaagt acggcactca gacggttctc catttccggg
      121 ttgatgtcac gcggaataaa ctccggagcg accttcggac gttcctgata tttcagtgag
      181 agtttgactg ccggatacgg tccgggcagg gtgacatagc aggtcagatc cggcagggac
      241 tgaatgtcgg aataactgac cagcgtctgg cgctccatat ccttaccggt cgataccccg
      301 tcacgtaccg gatcagcacc gtaggaatac tgttcgctgg ctttcaggtg ctctttctca
      361 ccaatttcac cggcagcgaa ctccgcaatc ttatggctgg gagaacggaa aaaggcacgg
      421 gtgttcatga cgtcaaacag cgtggcagcc gctttctcac cgtagatatc ttccagctgg
      481 gcataggact ggataccaaa cacataacag ccaccgaact tacgggcttc cggcaggatc
      541 tccaccaggt ccggcagttt gtgtaatgtg ggtaactcat cacagaagaa ccacacacga
      601 cgattacggt tttctcccat tgccagcaga ccacgaatgg caatggacag ccacatggag
      661 atcaccggct tcagggaggc atgagtgtcg gcattcgacg agataaacag ccagccgttt
      721 ttctgatcct cccggacacc acgcatccag tcacggatgg tgaagggctc accgttatgc
      781 tcaatcccct gcaggtaacg gatggctttc acatagttgg tcagcacagc acggatggag
      841 atggccgttt tctcgatttt ctcttccacc aggttggccg ccggtgaatt acgcaggtag
      901 gtacgcagtt tttcaatttt gatggaaagc agtgtgtcca ccagtttgct gtagctgcgg
      961 ttggggtcat tacgcatcag atacgccgct tccgcaaaaa tggtacgtcc tgaaccctgc
     1021 cagaacgggt cctcttttgt gcccatcggg atcagagtat ttgcggtatt atcaaaatcc
     1081 ggctgtgtca ggcactcctt ccacaaatcc caggcggcac agcgtgcacc ggggcggttc
     1141 agtagtgaaa ccggctctca gtattggggg cttataaagg cttgagcgga gtgcggggaa
     1201 actcgcacgc tccgttctta gggggcggcg gcgcagcaat gtgccgctgc tacccgaccc
     1261 gccagggcgg agacagcttc gctggctccg ttcacgacag ccagccccgc caaggggacg
     1321 cccgggaaag ccataaacag gagaggagag gaacggaaag aagaagagga agaagaacaa
     1381 taacaggcac ggtaaccaca gaaaaataac atcagaaaaa aatattccgg acacatcata
     1441 aacgggaagg gtaacgggcg acgcgtcagc gtggcccgct tcccttccgg caggcgtcag
     1501 cctgccaggg gttttcgcgc gggctgacag gcggcctcca ccgcctgccc tgtatcacct
     1561 gtgcctgctg ccctgaagga tgtagtgacc accgaggaaa accgcctgct gattcgccac
     1621 aaaatcgcag atttcatcaa caatctgcgc ggtggtgagc ggtttctgcc ccatttcacg
     1681 acgccgcgca ttaatcacgg ccctgtaatt acgcagaatt tcgcgggttt ctgtgctgat
     1741 attcatgaca tgtgctcccc gtccagcgcc tgtaacacaa cctccattgc ttcgcgttcc
     1801 tccctgacga atgcgtcacc gtcgcccccg ttctcactcc cgacggcata caggcgcttc
     1861 atttcactgc ctgccagcgc catcactttt tccagcatca ccgcagggac tgttacaaga
     1921 cggtttacgc gcttttcctc catcagttca ttgacaatat cggaaacaac tctgtcacat
     1981 gcgccatata caaaagcatc gtccagccgc tgcatgacgg tttccagctc atcgtctgtc
     2041 agactccagt cctgggctat gtgacgcaca tccccacggg tcagcaccac ggcaaaaaca
     2101 aattcactgt ccggcacgtc tgtatccagt acatcgcgga taatttcttt aacagtacag
     2161 tacatgttct cgtctccttt acaggggcgg cattgccgcc ccgtccgtta atcagtccag
     2221 tgccgcgtaa atcgcgttgc attcagggtg aaatacgatg tgacgccaca actgcgcatc
     2281 gcgcatccgg taaagctggg tcagtcctgc atccccgctg tcgtggtaca gccacaactg
     2341 gcggttaatc acaagagaag tcagaatgat gcctgcggca tcagcactca cggtacggtc
     2401 aaaccagttt gcaccgttca ccatgtggaa acggtcgcca tccggcatca tgtaaccgcc
     2461 accgtccggc aggcggatga agtgccagaa gccgccttca tattccggca tcattttttt
     2521 cgccagcgcg tacacatacg cctcggcata cagaaaatcg ttgccaaaca ggtaaggcag
     2581 aaaggagaga cggtcgcact cttcaacatt cagagtgaca ggtttcgcat attgcatgat
     2641 gttttcctcc gttgttcaca tattccgcga caccgggcgg tgccgtgcct ccataaaagc
     2701 cgggcgataa ggcggggatg cacagggcgc aagggaagcc gcagcctgac gcacacggct
     2761 ggcgggagtt ggagggtgag accgcgaaac ctgagcgggt gaacgaccgg aaaatcacgg
     2821 cagacgggaa ggagggaccg ggtgaccgca gcggcgaagc gtacccttgc atccccgcca
     2881 gcccggctaa gatggaggca cggcacagcc cgcacacgac gctgaggcta cagagctgaa
     2941 aacccacaag gccatgcccc gcatggccgg gtaaagcgga gccggcggcg acttcctttg
     3001 tgctctgtcc ggggaccggc aggaaaaagg cggggattta ccccataagc gctaacttaa
     3061 gggttgaacc atctgaagaa cgcgacgcct cggtgcctcg ttaagacgat gcctcgcgtt
     3121 cttcaattgc gttttgtagg ctgtcaggga tactgtccca cgaatggcca cctgtaagct
     3181 ccagatgacc atttttgtta ttctccacaa cgagttagtt cttcttttcg gatccggcac
     3241 ttctgggggg aaaatccagc gatggctgga ttatgtcgtc aattaaaaat gcggcgagta
     3301 gattagcaaa tatccacgct ttcgcgagtt caggttcctt tgcacgcaaa gcatccaagt
     3361 gcagcaaact tttgagccgc ttaaaagcca gttcaatttg ccatcgcaga cggtaacaat
     3421 cagccacttg ctctgctgaa tattcatctt ccgataatga tgttagcaat agcacatggc
     3481 ccgctgcttc cagcgtttcc gcctgaacta ctcgtccttt tcgacgattc tcgctgagca
     3541 gtcgggtttt actgattaat gctttttcgg gaggaagtga tacggcaatg agacgtgcag
     3601 gaaagggagc tccggctttt ttattacctg aattgcctat cattacagtg gtttcaccgt
     3661 tcttaccgca atccagcccg cgcagaaaat ccatcatgtc aaagcgcatt ccttctgcag
     3721 ttaaccagcg caatcctcgc cagtaaaccc ggacgatata atcagcttct ccaaaagcaa
     3781 gtgagcggat acattcggga cgcgaaccga atccccggtc agcaattcgt atctcgtctg
     3841 ccgtttgcgc aaatcgatcc agccgttcag cgtctctgct gtcggttagc tcaaaatcag
     3901 tgaactgaca ggtatgagga tcatatccca tatgtagtcg ccattcagcg ctgccgcccc
     3961 cgggcgcgct gattgctgtt ccatcgacaa gacgcaatca aggtgcggcg ggagtcgatg
     4021 gcatgcgcgt aagtgaactg ccggactacc tgaggcacca ctggccagag ctgaaagcgc
     4081 aactgctgtc cggcagatac cgcccatccc ctgtgagaag agtatccata ctgaagcccg
     4141 gcggcggcga acgtctgctg ggcatccaga tggtggtgga tcgcttcatc cagcaggcta
     4201 tgatgcaggt attgcaggca ctgtgagata gctcgttcag cgacaacagc tacgggttcc
     4261 gtcccggtcg ttcggcccac caggcagtga tacaggccca ggaatatatc ggggccgggt
     4321 atcactgggt ggtcgacctc gatctggaga agttcttcga ccggataaat cacgacgtgc
     4381 tgatgagccg gatagagaaa cgagtatcgg ataaacgggt gttgtcactt atccgcaggt
     4441 tcctgaatgc aggggtgatg gacgcaggtc tggtaaggcc ggtgacagaa ggaacgccgc
     4501 agggcggcgt gatctcgccc ctgctctcaa atctgttcct gcactatgcc tttgatatgt
     4561 ggatgcaacg tcagtgtccc gacgttccct tcgaaaggta cgccgatgat agtgtgccac
     4621 gaaggcgcgc acc
//
LOCUS       DADLSB010000239         4616 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.239, whole genome shotgun sequence.
ACCESSION   DADLSB010000239 DADLSB010000000
VERSION     DADLSB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.239"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            20..178
                     /locus_tag="JN331_003971"
                     /pseudo
     CDS             20..178
                     /locus_tag="JN331_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(234..1169)
                     /locus_tag="JN331_003972"
     CDS             complement(234..1169)
                     /locus_tag="JN331_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7261939.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKKINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1180..1413)
                     /locus_tag="JN331_003973"
     CDS             complement(1180..1413)
                     /locus_tag="JN331_003973"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261940.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSHIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(1406..2680)
                     /locus_tag="JN331_003974"
     CDS             complement(1406..2680)
                     /locus_tag="JN331_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7261941.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELQ
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKAALWLSWMYYNGLGETLDCDKV
                     WDYYEKGAGKYSMKFNKDEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPK
                     ICNVSIEINADKISSITNLQLTLELKGGGGSE"
     gene            3073..4443
                     /locus_tag="JN331_003975"
     CDS             3073..4443
                     /locus_tag="JN331_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7261942.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
BASE COUNT         1229 a         1074 c          938 g         1375 t
ORIGIN      
        1 tttttgaaca tgtaaaaaaa ttcaccaggc attaccaatt aaaggggttt atcttgatgc
       61 tgaccctgcc gccattaact tttatgctcg tctcggcttt gttcagcttt cagcgacacc
      121 aaatgctttt ggtgctgtac ctatgttttt ggcgattcag catattctcg cggcttagat
      181 tattatcgcc acccgctagg gcaagttttg ccttagccgg gggctgagca gcgttaccac
      241 ttttgttcaa ttgcccccgc tttcagcaac tccacatcct tgccgttaat agttgccgtt
      301 gcggcaacga ttgtggtcct tacagttgca caagattttc gctcaaattc cggtttataa
      361 agaataagag acgaattact ggtaaatgaa ccaaataggt aatcagtaaa gctaatattc
      421 agccggttta aaccgaaagg cgcgaagcca accgtttggt taatcttctt accggatggg
      481 cgacgaatgc tgaatgtcac gcgaagattg gccacttcgc ctaatttatt ggtggtggcg
      541 aagaggctat ccatgcagga aaaaccttta tctaaaaaat tatcccgcat cccagaacgg
      601 gtaaccgtga tcacgggtaa cgtatcctca accgtttttc tgtcaatcat gtcttttcga
      661 caagtagcca gaaagtcttc cgggccacca taaatgtgga cacctttatc aaggtattcc
      721 ttcgctttct tacaatctac ctcaacatac tgaccattgg cgtacatcat gcccaacctg
      781 gaccatgcta aattcatacc ttgctgcgcg gcctgattaa atagtgcata cgctttggca
      841 taatccaccc gcacctgctc accgtcgtag tagatactcc ccagcgctaa cggagcttct
      901 ctgtcacctt gtttagcggc taactcgaac catttaatcg cttcctgaac atcaggtttg
      961 gtatcatagc gaggctgata atattcccag cctaacaggc tctgcgcgtc gatatctccc
     1021 gattcagcca ttttatgcag tgtatttata acatcgggag cagatttacc accgtatatg
     1081 ctgtcataca attcatgcgc tttcttactt gccaccacgc ctgatgaaac tatcagcatt
     1141 aaagaaaata atattctatt aggcaacata aaatatctct tatttatctt gtacctgaat
     1201 atgctcttct tttaataaat cacgttgttt tccattcagt atcgctgtcg cagatttaat
     1261 agaataagtc aaatcatgaa aatcgcataa attacgatta tacattggga tgtctatata
     1321 gctttttctt atatgactgg aattattata catatccaca ccgagcgtat tcaaactaaa
     1381 aggagggaca gaaagagtat attctttact cactccctcc cccccctttt agctcaagcg
     1441 tcaactgcaa attagttata ctactaattt tatctgcatt aatttcaata ctgacattgc
     1501 atattttggg tttttcattt gcgttaaagc cataggtccc gtaatgtttt aacgtgatgt
     1561 aaggtaaagt atcaccctgt gtttctcggg ttttctgatc ttgattacaa cgggttaaat
     1621 attcatcctt attgaatttc attgaatatt tccccgcacc tttttcgtaa taatcccata
     1681 ccttatcaca atcaagcgtt tcacctaatc cgttgtaata catccaactt aaccacaatg
     1741 cggctttttt tgccccttgc tgttcagcct tgtcaaaaag catgctcgct ttagaataat
     1801 caacctcacc ggtcaaacca gaatagtata tattccccag ggccactaat gctttttcgt
     1861 cattcttatt tgcagctaat tcgtaccatt ttttggcttc gacagcatca atctttaaac
     1921 cagcactgcc attttcgtag gtatcgccca tggtatactg cgccatcgga taaccttttt
     1981 ctgccgcagt acgaaaccat tgcagtgctt tctttatggt gtctaaagaa cgtctatcat
     2041 cagaaggtga aaaatcatcg agataaagat ttccgacaat ataatccatt tcggggtcgc
     2101 cttcctgcgc ccatagagtc gccagacgca gaatttcagg tttactgaaa ccaattcgcc
     2161 cccaaaaaag caaatcattc tcgggtgcct gaccgacata acgactgaca aattcaatta
     2221 aactcgcccg tttcgttttg tctgtctcag ccccggattt caattcatag gcctgattga
     2281 ttattttatt catcaaatca ggcggaatat taagatcgtc agttttcgac ttactggcat
     2341 acttctgtgc tcgtgtcaca tccagggtga cgcctttacc atattgaaag aaataacgtg
     2401 cattataaca accgtattcg cccccctggt tacaggcttt ctcataccac tgaatcgcct
     2461 ttgggatgtc aacagatact gtcactccgt gttcataaat aaatcccagt agcactagcg
     2521 cctgcgtgtt attggtatca gctaattttt gcagttcttt taacgccgtc tgatctttat
     2581 tgacaatacg ttcatacagt tgatcaatat ctgttgaagc atgacaaaaa gaagccagag
     2641 ataaacccaa tgataataaa ataactttac cagaccacat acattcctcc ttgatatatt
     2701 caaccaccaa acatccacct catgaataaa tacgctgcca taacggccca ggtaataagt
     2761 aaacatttaa gctgaaacag cataaccagt gcctccaacg cttctattta ttgcgataaa
     2821 ataacaccat cctttgataa aaagtcacag cccatcaata aatccaaaaa taatgtcatt
     2881 agttgcagga taaaatcatt tgagcttttc atttgttata tttaacgcat gatgtattaa
     2941 tggcaagaat atgagagaaa ctcatacctg acgtaaaaat cactctctta tgtaatattt
     3001 acttattaac actctgttta cctaacatgc gctccgcact acactatgtg ggcaattttt
     3061 taagaggtgg taatgaacga ttataaaatg acgccaggtg agcggcgcgc gacctggggt
     3121 ttagggaccg tattttcatt gcgcatgctg ggcatgttta tggtactgcc ggttctgact
     3181 acgtacggta tggcactgca aggcgccagc gaagcattaa tcggtattgc cattggtatt
     3241 tatggtctga cccaggccgt ttttcagatt ccgtttggcc tgctttccga ccgcattggt
     3301 cgaaaaccat taattgtcgg tgggctggca gtgtttgccg ccggtagcgt tatcgctgcg
     3361 ctttctgact ccatctgggg aattattctg ggccgggcgc tacaaggctc cggtgcgatt
     3421 gccgctgccg ttatggcgct gctttccgat ctcacgcgcg aacaaaaccg taccaaagca
     3481 atggcgttta tcggcgtgag ctttggcatt acctttgcca ttgcgatggt gcttggcccg
     3541 atcatcactc acaaacttgg gctgcacgcg ctgttctgga tgatcgctat tctggcaacg
     3601 accggcattg cgttgaccat ttgggttgtg cccaacagta gcactcacgt acttaatcgt
     3661 gagtccggaa tggtgaaagg cagtttcagt aaagtgctgg cggaaccgcg gctgctgaaa
     3721 ctcaactttg gcattatgtg tctgcatatt ttgctgatgt cgacgtttgt tgccctgccc
     3781 ggacaactgg ctgatgcggg gttcccggcg gctgaacact ggaaggtcta tctggcgaca
     3841 atgctaatcg cctttggctc ggtcgtgcct ttcattatct acgctgaagt taagcgcaaa
     3901 atgaagcaag tctttgtctt ctgtgtcggg ttgatcgtgg ttgcggaaat tgtgttgtgg
     3961 aacgcgcaaa cgcagttctg gcaactggtg gtcggcgtgc agcttttctt tgtggcgttt
     4021 aatttgatgg aagccctgct gccctcactt atcagtaaag agtcgccagc aggttacaaa
     4081 ggtacggcga tgggtgttta ctccaccagc cagtttcttg gcgtggcgat tggcggttcg
     4141 ctgggcggct ggattgacgg catgtttgac ggtcagcggg tatttctcgc tggcgcaatg
     4201 ctggccgcag tgtggctggc agtcgccagt accatgaaag aaccgccgta tgtcagcagt
     4261 ttgcgcattg aaatcccaga aaacgtaatg gcagatgaaa aactggaaaa atgcttactg
     4321 acaacgcctg gtgtcaaaga tgtactgctg gcgaagttag aacattgcgt ctatgtgaaa
     4381 gtagacagta aaatcaccaa tcgttttgaa atagagcgag taatcaacga agtatcttat
     4441 taaataataa gcacggtgct attcatgaaa aataaaatag caccgtactt cgtaacacca
     4501 taatccttta aaccctccat tattaatact tcgcttaatt ccatataact ggacgattac
     4561 attagaagta aactatactt tttatgaaat atcagggata aattattcac atggaa
//
LOCUS       DADLSB010000240         4613 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.240, whole genome shotgun sequence.
ACCESSION   DADLSB010000240 DADLSB010000000
VERSION     DADLSB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.240"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(361..837)
                     /locus_tag="JN331_003976"
     CDS             complement(361..837)
                     /locus_tag="JN331_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY7261943.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(896..2185)
                     /gene="bioA"
                     /locus_tag="JN331_003977"
     CDS             complement(896..2185)
                     /gene="bioA"
                     /locus_tag="JN331_003977"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY7261944.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGTMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2272..3312
                     /gene="bioB"
                     /locus_tag="JN331_003978"
     CDS             2272..3312
                     /gene="bioB"
                     /locus_tag="JN331_003978"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY7261945.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3309..4463
                     /gene="bioF"
                     /locus_tag="JN331_003979"
     CDS             3309..4463
                     /gene="bioF"
                     /locus_tag="JN331_003979"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY7261946.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4450..>4613
                     /locus_tag="JN331_003980"
     CDS             4450..>4613
                     /locus_tag="JN331_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="methyltransferase; acyl carrier protein involved in
                     an unidentified step in the synthesis of pimeloyl-CoA, a
                     biotin precursor; member of the bio operon (bioABFCD); in
                     Escherichia coli, bioC-null mutants require biotin for
                     growth; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY7261947.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPG"
BASE COUNT         1089 a         1228 c         1274 g         1022 t
ORIGIN      
        1 gcatcatttc tctgcgctta tcgagatact gagcatggtt gtaaattcag ctaacccagt
       61 gcgttgacaa aaaattagcg caagaggaca aaaaatcacc ttgcgctaat gctctgtatc
      121 aggtcactaa taccatctaa gtggttgatt catagtgact ggatatgttg tgttttgcag
      181 tattatgcag tctatttttt aagctaaatg tattataatg cattgatatt aatgattttt
      241 actgtttctc gttcagcttt tttatactaa cttgagcgaa acgggaaggt aaaaagacaa
      301 aaagttgttt ttaatacctt taagtgatac cagatggcat tgcgccatct ggcagagtga
      361 ttaactaaac atcgcggtaa tcgatgcgct tgccagagag tggaaatgaa cgttaaaccc
      421 gaccatcgcg ccgctggctc cttcatcgac atcaatacgc tctacatcca gcgcgtgaac
      481 ggtaaaaatg tagcgatggg tttcgccttt cggcggcgct gcgccatcgt atccggtttt
      541 accaaaatcg gtacgcgtct gcaaaacgcc gtctggcatt gctaccagac cagagccaaa
      601 cccttgcggt aatacgcggg tatcagcggg taaattaaca actacccagt gccaccagcc
      661 ggagccggtt ggcgcatccg ggtcgtagca ggtgacaaca aaacttttcg ttcccgcagg
      721 aacatcatcc cacgccagat gcggtgaaat attatcgcca tcgtaaccca tgccgttaaa
      781 gacatgacga tgcggcaact tatcgccatc gcgcagatcg ttactgatga gtttcatgaa
      841 ccctcctttc ttgtttgcag aaagtgtagc cagaaaccct cacgctgact tcccgttatt
      901 ggcaaaaaaa tgtttcatcc tgtaccgcgc ggttaaccgc tgcggtcaga cgctgcaact
      961 gttgcgggag aataatatag ggcggcatca ggtaaatcag tttgccaaaa ggccggatcc
     1021 ataccccctg ttcgacaaag aatttttgca gcgccgccat attcaccgga cgagtggttt
     1081 cgaccacacc gattgccccc agtacgcgca catcggcaac catttcggca tcacgggctg
     1141 gtgccagttg ctcgcgcagc tgcacttcaa tagccgccac ctgctgctgc cattcgccgg
     1201 attcgataat cgccaggctg gcgtttgctg ccgcgcaggc cagcggattg cccataaaag
     1261 ttggcccatg cataaagcag ccggcttcgc cgttactgat ggtttctgca acctcgcgcg
     1321 tggtaagtgt ggcggaaagg gtcattgtgc cgccggttaa ggctttaccg aggcacaaaa
     1381 tgtccggcgc gatttctgca tattcacagg caaacagttt gccggtacga ccaaatccgg
     1441 tggcgatctc gtcggcaatc agcaagatac cttcgcgatc gcacattttg cggattcgtt
     1501 ttaaccattc cggatggtac atgcgcatcc cgcctgcgcc ctggacaatc ggctcaatga
     1561 tcaccgctgc gatttcatga cgatgcgccg ccatcaggcg ggcaaaacca accatatcgc
     1621 gttcatccca ttcaccatcc atgcggcttt gcggggcggg agcaaacaag ttttctggca
     1681 gatagccttt ccacaggctg tgcattgagt tgtccggatc gcacaccgac atcgtgccaa
     1741 aggtatcgcc atgataacca ttgcggaagg tcagaaaacg ctggcgcgct tcgcctttgg
     1801 cttgccagta ctgcaacgcc attttcatcg ccacttccac cgctacggaa ccggagtccg
     1861 cgagaaaaac gcactccagc ggttgcggcg tcattgccac cagtttgcgg catagctcga
     1921 tggcgggcgc atgggtgata ccgccaaaca tcacatgcga catggcatca atttgcgtct
     1981 tcatcgccgc attaagctgc gggtgattgt agccgtggat cgccgcccac caggacgaca
     2041 taccgtcaac caggcgtctg ccgtcagaca aaattagctc gcaaccttcg gcgctcgcca
     2101 ccggataaac cggcagaggg gaggtcatgg atgtgtatgg gtgccagata tggcgttggt
     2161 caaaggcaag atcgtccgtt gtcataatcg acttgtaaac caaattgaaa agatttaggt
     2221 ttacaagtct acaccgaatt aacaacaaaa aacacgtttt ggagaagccc catggctcac
     2281 cgcccacgct ggacattgtc gcaagtcaca gaattatttg aaaaaccgtt gctggatctg
     2341 ctgtttgaag cgcagcaggt gcatcgccag catttcgatc ctcgtcaggt gcaggtcagc
     2401 acgttgctgt cgattaagac cggagcttgt ccggaagatt gcaaatactg cccgcaaagc
     2461 tcgcgctaca aaaccgggct ggaagccgag cggttgatgg aagttgaaca ggtgctggag
     2521 tcggcgcgca aagcgaaagc ggcaggatcg acgcgcttct gtatgggcgc ggcgtggaag
     2581 aatccccacg aacgcgatat gccgtacctg gaacaaatgg tgcagggggt aaaagcgatg
     2641 gggctggagg cgtgtatgac gctgggcacg ttgagtgaat ctcaggcgca gcgcctcgcg
     2701 aacgccgggc tggattacta caaccacaac ctggacacct cgccggagtt ttacggcaat
     2761 atcatcacca cacgcactta tcaggaacgc ctcgatacgc tggaaaaagt gcgcgatgcc
     2821 gggatcaaag tctgctcagg tggcattgtg ggcttaggcg aaacggtaaa agatcgcgcc
     2881 ggattattgc tgcaactggc aaacctgccg acgccgccgg aaagcgtacc aatcaacatg
     2941 ctggtgaagg tgaaaggcac gccgcttgcc gataacgatg acgttgatgc ctttgatttt
     3001 attcgcacca ttgccgtagc gcggatcatg atgccgacct cttacgtgcg cctttctgcc
     3061 ggacgcgagc agatgaacga acagactcag gcgatgtgct ttatggcagg cgcaaactcg
     3121 attttctacg gttgcaaact gctgaccacg ccgaatccgg aagaagataa agacctgcaa
     3181 ctgttccgca aactggggct aaatccgcag caaactgccg tgctggcagg ggataacgaa
     3241 caacagcaac gtcttgaaca ggcgctgatg accccggaca ccgacgaata ttacaacgcg
     3301 gcagcattat gagctggcag gagaaaatca acgcggcgct cgatgcgcgg cgtgctgccg
     3361 atgccctgcg tcgccgttat ccggtggcgc aaggagccgg acgctggctg gtggcggatg
     3421 atcgccagta tctgaacttt tccagtaacg attatttagg tttaagccat catccgcaaa
     3481 ttatccgtgc ctggaagcag ggggcggagc aatttggcat cggtagcggc ggctccggtc
     3541 acgtcagcgg ttatagcgtg gtgcatcagg cgctggaaga agaattggcc gagtggcttg
     3601 gctattcgcg ggcgctgctg tttatctctg gttttgccgc gaatcaggca gtcattgccg
     3661 cgatgatggc gaaagaggac cgtattgttg ccgaccggct tagccatgcc tcattgctgg
     3721 aggctgccag tttaagcccg tcgcagcttc gccgttttac tcataacgat gtcgctcatc
     3781 tggcgcgact gcttgcttcc ccctgtccgg ggcagcaact ggtggtgaca gaaggcgtgt
     3841 tcagcatgga cggcgatagc gcgccactgg cggaaatcca gcaggtaacg caacagcaca
     3901 atggctggtt gatggtcgat gatgcccacg gcacgggcgt tatcggggag caggggcgcg
     3961 gcagctgctg gctgcaaaag gtaaaaccag aattgctggt agtgactttt ggcaaaggat
     4021 ttggcgtcag tggggctgcg gtgctttgct caagtacggt ggcggattat ctgctgcaat
     4081 tcgcccgcca ccttatctac agcaccagta tgccgcccgc tcaggcgcag gcattacgtg
     4141 cgtcgctggc ggtcattcgc cgtgatgagg gggatgcacg gcgcgaaaaa ctggtgtcac
     4201 tcattgcgcg ttttcgtgcc ggagtacagg atttgccgtt tacgctggct gattcatgca
     4261 gcgccatcca gccattgatc gtcggtgata acagccgtgc gttacaactg gcagaaaaac
     4321 tgcgccagca aggctgctgg gtcacggcga ttcgcccgcc aaccgtaccc gctggtactg
     4381 cgcgactgcg cttaacgcta accgctgcgc atgaaatgca ggatatcgac cgtctgctgg
     4441 aggtgctgca tggcaacggt taataaacaa gccattgcag cggcatttgg tcgggcagcc
     4501 gcacactatg agcaacatgc agatctacag cgccagagtg ctgacgcctt actggcaatg
     4561 cttccacagc gtaaatacac ccacgtactg gacgcgggtt gtggacctgg ctg
//
LOCUS       DADLSB010000241         4449 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.241, whole genome shotgun sequence.
ACCESSION   DADLSB010000241 DADLSB010000000
VERSION     DADLSB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.241"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1229)
                     /locus_tag="JN331_003981"
     CDS             complement(<1..1229)
                     /locus_tag="JN331_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7261948.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNVGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRS"
     gene            1446..2369
                     /locus_tag="JN331_003982"
     CDS             1446..2369
                     /locus_tag="JN331_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7261949.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(2407..4047)
                     /gene="trg"
                     /locus_tag="JN331_003983"
     CDS             complement(2407..4047)
                     /gene="trg"
                     /locus_tag="JN331_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY7261950.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRNRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
BASE COUNT         1075 a         1170 c         1045 g         1159 t
ORIGIN      
        1 tgactgcgtg tctgcgtttc attacccaga tttgactgga agatccccgc cgcgctaacg
       61 ggcaagaaat cttcataggt gatgggttgc gccactaccc aaccacgttc aattaagggc
      121 tgtggatcgt ctccaggatg aatcgcctga cgatgcgcct cacccgaagg cgtcagacgg
      181 taccggaacc atgccaaccc ttgctgacgc attaaaaact cactgtcagg aaaagtgcgg
      241 aaggtttcct gtaaatgcat ttggtgagtg agattatcct gcccggttcc aacgttacgc
      301 agaagatcat catacagttg tcgccctttc ggcgttaatg ccacgccacg ctgctcaatt
      361 tcaccaaagc gcgcggtatg cgtgccctgt ttctgccccg caaacaacac cgtctcttcc
      421 agtgctttaa agctggtctg ccgtagtaaa atcggtacct cgcggcgcgg cggcccctcg
      481 atgagaattt tgggttcaat tccgcattca ggcatcatcg actgcacccg gtcaatatcc
      541 agcgtacgtg gcgtcaggtg gttgatatgg catccaggaa aacagaccac atcagcaatc
      601 aaccgatgtt cgttgtgcaa ggcgcgatag gtttcttcat ctaccgttgc taactggtgc
      661 cagcgaaacg tttccagggc ttcctgcaca aactcctgtg cctgtgtttc gttaaaaccg
      721 ccctgctgct catattcctc taacagttgt cgacaacgtg gggtgaagat atcgcgctga
      781 cgcagaatct ccgccgcttt ctggcgcaaa atttcgttct cgataagctc aaggcggagt
      841 aaggatgtaa aaacgcggaa gggattacgc gccagagaag catcatcaat gggccgaaat
      901 gctgtcgaat gtaccggcac ccctgcctgc gagagatcgt aatagctcac cgggtacatc
      961 cccataatgg caaacatccg ccgaagagta gcaagctctt gtgcagtccc aacgcgaatc
     1021 gccccatgac gttcaacatt cagtcgcgcc agctcgtctg catttaccat tttttcgtgc
     1081 agttgaggat tgttttccag cacagccaga ttcacatcag ctaccagttc cagcagcgtg
     1141 ccatattgcg gaacttcttg ctggtacatg gctgacattg cctgcgaaaa ctgttcccga
     1201 atctcatccg ccgtgatgct gttcgccata gtgtctgcct ccagtggtca gtaatctgga
     1261 gtgtaggtaa ccgcattcac tctggcggga agaatttaca aactgtgatc tcgccgcgaa
     1321 aacatcaata ttatccattt tgctgtaaca taattgcttt aattgttaat aatattttgc
     1381 aatcaagtta tcataatcaa acaacttcac ttgtcagcga caccgcttcg tttttaacat
     1441 cgcttatgga aaaaaatagt ctgtttagtc agcgcatccg tttgcgccac cttcatacat
     1501 tcgtagctgt cgcacaacaa ggaactttgg ggcgcgcggc tgaaaccctt aatttgagtc
     1561 aacctgcgct ctctaataca ttgaatgaac tggagcagct gacgggcgct cgcttgtttg
     1621 agcgtggtcg tcagggggcg caacttacct tacccggcga acaattttta acgcatgcag
     1681 tcagagttct tgacgccatc aacactgccg gacagtcgct tcatcgtaaa gaaggtctta
     1741 ataatgatgt cgtcagggtt ggtgcactac ctactgcggc actggggata ttaccttcgg
     1801 ttataggtca gtttcatcag caacaaaaag agacgacctt gcaagttgcg acaatgagta
     1861 accctatgat tctggcgggt ttaaaaaccg gggaaatcga tatcggcatt ggtcggatgt
     1921 cagatcctga actgatgacc gggcttaatt acgaactgct gtttcttgaa tcgttgaagc
     1981 tggttgtccg ccctaatcac ccgctacttc aggagaacgt aacgctaagc cgggtgctgg
     2041 aatggccggt cgttgtatca ccagaaggca ctgcgccacg ccagcattca gatgcattag
     2101 tgcagagcca gggctgtaaa attccttcgg gttgtatcga aacgctgtct gcttcgctat
     2161 ctcgtcaact taccgttgaa tacgattacg tgtggtttgt cccttctggc gctgtaaaag
     2221 acgacctgcg tcatgccacg ctggtggccc tgcctgttcc gggacatggt gcaggcgaac
     2281 cgattggaat actgacccgc gtagatgcga cgttctcttc tggttgccag ttgatgatta
     2341 acgctattcg aaaatcaatg ccgttctgaa aggtgaaggg atctgtcgat ccctccttga
     2401 acattttcac accgtagcga aactaactgg ttcacccgct ccgcgaggtt ctgccgacac
     2461 agaatgtttg ttcagacgga atacgttcac cgcctcagtt aatcgtgcag cctgttcttc
     2521 aagggacacc gccgctgctg aggcctcttc taccagagaa gcattctgtt gcgtcacctt
     2581 atccatttca gaaatcgcct ggctaacctg agttatgcct ctactttgtt catccgaggc
     2641 ggcggcgatt tcctgcatga tatgtgtgac actcgcgacg gcatcaacaa tagtgctcat
     2701 ggttttccct gccgttgcca cctcatccga ccccaggtca attaacctga ctgattcact
     2761 gatcaagcct tcaatctctt tcgctgcctg agcgctgcgg cttgcgagtg tccgtacttc
     2821 gctggcgaca acggcaaatc cacgcccttg ctcacccgct cgcgcggctt caacggcagc
     2881 attcagtgcc agaatattcg tctggaaagc aatactgttg atgacggcgg tgatctcaga
     2941 aattttcttc gaactcgtgg agatagcgcc catcgttttt actacaccgg aaaccgtctg
     3001 cccgccatcg ctggctttaa tagaagcctc ttgcgccagt ttgctggcat gatgcgcgtt
     3061 atccgcattc tgtttcaccg tcgcagtgag ttgctccatg ctggcggcag tttgttcgat
     3121 agccgccgct tgttcttcgg tgcgagatga cagatccgca ttgccagctg aaatttcgct
     3181 ggtgccacga taaatctctt ccgcgccctg tcgaacagtc cctactgtca tccccagtga
     3241 atgctgcatt tgctgtaaat gacgacttaa gcgaccgatt tcattacgac ccgccggttc
     3301 atcattcatc gtcagatcgc cactggcgat tttttcaatc cgttgtgcgg catgttgcag
     3361 tggacgaatg acgatccgac gtagcaccat aaatgttatc agcgtcatga ccagggcaag
     3421 cacaaacgcg ccaatcatga acatcccacc caggcggttg cgctgatggg ccagttccgc
     3481 cagttggttg gctctggtgc tacgtatctt aacggctttg ttcaaaatat cggtataagc
     3541 attatccagc ggtcggatct gctcactttc atgattgata atcgcttcaa acatgccatt
     3601 tttggcatat ttcaacatag gttgcatacc cgtgatataa gcctgaaagc gttgatttaa
     3661 ttcagtgtcg agggcttcat cagcaggtgt tttcaccggt cgattctgat aagcacgata
     3721 accttgctgc gactgtttaa tctccgattc ggcttgcgca atatttcgct tcattgcttc
     3781 catttccgca atacgactcg cagccccggc ctgaatcata ttgatccggg ctgaacgcaa
     3841 aaaatctgaa ctgttcgcta acccggtccg aatctcaatc tccgcagtaa catcacgctg
     3901 atcgcgatcg gcctgccaga ggaaatagcc agccagagca gaacttaatg cgaatagaac
     3961 taagataccg ccaagaatgc aggcaaataa cggaaccagc ctgatgtgat gcaaaaaacc
     4021 taatcgctgt gagggagttg tattcattac cgttgtctct cgtccaggtt tactcctgat
     4081 agaaagtcat cggcacttga cggtaattac ttatgaccaa aaacctcttt gcgtcacatt
     4141 tttacaacaa ttattgcaga gaaaattaat gctattggaa tgaagagagt aacgaatggc
     4201 taaactggct aacccgaatg acgaaaaatt ctggaaaaca gcacgcaatt tcgtgaagtg
     4261 attattttcg tccctaagga aacctcatag gatgcctgcg ggtgcctgag actttctgtt
     4321 ttcaggctaa ccgggaatca gaaagacgaa ggccccgacc gatatttcta tcaactgtac
     4381 tgcacccatt ttgttggacg atgaaatgga atagccccta atatgtcaaa gccaaaatac
     4441 ccttttgaa
//
LOCUS       DADLSB010000242         4403 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.242, whole genome shotgun sequence.
ACCESSION   DADLSB010000242 DADLSB010000000
VERSION     DADLSB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.242"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..813)
                     /gene="mutY"
                     /locus_tag="JN331_003984"
     CDS             complement(<1..813)
                     /gene="mutY"
                     /locus_tag="JN331_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7261951.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLR"
     gene            974..1693
                     /gene="trmB"
                     /locus_tag="JN331_003985"
     CDS             974..1693
                     /gene="trmB"
                     /locus_tag="JN331_003985"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY7261952.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            1693..2019
                     /locus_tag="JN331_003986"
     CDS             1693..2019
                     /locus_tag="JN331_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY7261953.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGRICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            2203..2922
                     /locus_tag="JN331_003987"
     CDS             2203..2922
                     /locus_tag="JN331_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY7261954.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            3098..4144
                     /gene="ansB"
                     /locus_tag="JN331_003988"
     CDS             3098..4144
                     /gene="ansB"
                     /locus_tag="JN331_003988"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY7261955.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            4261..>4403
                     /locus_tag="JN331_003989"
     CDS             4261..>4403
                     /locus_tag="JN331_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY7261956.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDA"
BASE COUNT         1128 a         1090 c         1184 g         1001 t
ORIGIN      
        1 ccgcaaactt tcttcgtcgg caaactgcgg gaaacagtat aaaccgcccc acaatccgct
       61 cggcggacgc tgcgccagca atacttcatc ttcgtgctgt aacaggagaa agtagccggt
      121 gcgctccggc agcgtctgtt tcggtttttt gcccggataa agcgaccagc tattgttggt
      181 ggcggcaata catccgtttt gtagcggaca gagcgaacat ttcggcttcg aacgcgtgca
      241 aatcatcgcg cccaaatcca tcatcgcctg attaaaccgt tccacgccga ccgcgggcgt
      301 cacctgctcg ctcaaactcc atagtttatt ctcgacctct tttttcccag gccagccgct
      361 tacagcatag cagcgcgcca gcacgcgttt gacgttaccg tcgagaatcg gaaagtgctt
      421 acccagagaa agcgagagaa tcgcgcctgc ggtggaacgc ccgacgcccg gtaacgccgc
      481 gacttcttca aaggtttccg ggaatttacc gccgtgtaag gtcgccactt gttgtgccgc
      541 tttatgcagg ttgcgcgcgc gggcgtaata gccaagcccg gtccacaagt ggagaacttc
      601 gtcgagcggc gcattggcga gatcggtcac cgtcgggaag tgcgccataa agcgttcaaa
      661 ataggggata acggtcgcaa cctgagtttg ttgcaacatc acttctgaga gccatacttt
      721 gtagggcgtc ttgtcaattt gccagggcag cgttttccgc ccgtatttat cgtaccagtc
      781 cagaacctgg gctgaaaatt gcgacgcttg catggtcacc gacttcactg ttgttggggg
      841 caagattgca gcacagcgac ggcagggtgt aaaccggaac tttccgcagc cacggcctta
      901 tcatgcttgc atccggcaac taactttgga taatgcccgt tttcagaaca ctttcacaag
      961 cgactaaatc tttatgaaaa acgacgtcat ttcaccggaa tttgatgaaa acggccgccc
     1021 actgcgccgt atccgtagtt ttgtgcgccg ccaggggcga ctgaccaaag gccaggaaca
     1081 tgcgctggaa aactactggc cggtgatggg cgttgagttc agtgaagata tgctggattt
     1141 ccccgcgctt tttggccgtg aagcgccggt gacgcttgag attggttttg gcatgggggc
     1201 gtcgctggtg gcaatggcta aagatcgccc cgagcaggac ttcctcggca ttgaagtgca
     1261 ttcaccgggc gttggtgcgt gcctggcttc tgcgcatgaa gaaggtttaa gcaacctgcg
     1321 cgtgatgtgt cacgatgcgg ttgaagtgct gcataaaatg attcctgaca attcattgcg
     1381 catggtgcag ctctttttcc ctgacccgtg gcacaaagcg cgccataata aacgccgtat
     1441 cgttcaggtg ccgtttgccg aactggtaaa aagcaaactg cagctggggg gcgtattcca
     1501 tatggcgacc gactgggaac cttatgcgga acatatgctt gaagtgatgt cttctattga
     1561 cggttataaa aacctgtcag agagcaatga ttacgtaccg cgtccggcat cacgtccggt
     1621 gacgaaattt gaacaacgtg gtcatcgtct tggtcacgga gtatgggact taatgttcga
     1681 gagggtgaaa taatggcaaa gaaccgtagc cgtcgtctgc gtaaaaaaat gcacatcgac
     1741 gaattccagg aattaggatt ttcggtggca tggcgattcc cggaaggtac atcggaagaa
     1801 cagattgata aaatcgttga tgactttatt aacgaggtta tcgaaccgaa caaactggcc
     1861 tttgacggca gcggttatct ggcctgggaa ggtcggatct gcatgcagga aatcggcaaa
     1921 tgcaccgaag aacatcaggc gattgtgcgt aagtggctgg aagagtgcaa actggatgag
     1981 gtacgcacca gcgaactttt cgacgtttgg tgggactaag aaagcatacg ggcgatgaca
     2041 aatgcaaaac tgcctgatgc gctacgctta tcaggcctac agagatgcac gatcgagtag
     2101 gcgggataag gcgtttacgc cgcatccggc atggaaaacg cgtactttgt tatcaatctg
     2161 gggccagcat atgctggcct gatttgttct tgagggaaga ctatgatgcg caaaatgctg
     2221 ctggcggcag cactttcagt gacggcaatg accgctcacg ccgactacca gtgcagcgtc
     2281 acgccgcgtg acgatgtgat tgtcagcccg caaaccgtgc aggtgaaggg cgaaaacggc
     2341 aatctggtga tcacgccaga cggcaacgtg atgtataacg gtaagcaata ttccctgaat
     2401 gccgcccagc gcgagcaggc gaaggattat caggctgaac tacgtagcac gctgccgtgg
     2461 attgatgaag gcgcgaaaag ccgcgtcgag aaagcccgta ttgcgctgga taaaattatc
     2521 gttcaggaga tgggcgacag cagcaagatg cgcagccgtc tgaccaaact tgatgcgcag
     2581 cttaaagagc agatgaatcg cattatcgaa acgcgcagcg atggcctgac gttccactat
     2641 aaagccattg atcaggttcg cgccgaaggc cagcaactag tgaatcaggc aatgggcgga
     2701 attttacagg acagcattaa tgaaatgggc gcgaaagcgg tgctgaaaag cggcggtaac
     2761 ccattacaga atgtgctggg aagcttgggc ggtctgcaat cctcaatcca aaccgagtgg
     2821 aaaaagcagg aaaaagattt ccagcagttt ggcaaagatg tttgtagccg cgttgtgact
     2881 ctggaagata gccgcaaagc cctggtcggg aatttaaaat aatcctctat tttaagacgg
     2941 cataatactt ttttatgccg tttaattctt cgttttgtta cctgcctcta actttgtaga
     3001 tctccaaaat atattcacgt tgtaaattgt ttaacgtcaa atttcccata caaagctaag
     3061 ggataatgcg tagcgttcac gtaactggag gaatgaaatg gagtttttca aaaagacggc
     3121 tcttgccgca ctggttatgg gttttagtgg tgcagcattg gcattaccca atatcaccat
     3181 tttagcaacc ggcgggacca ttgccggtgg tggtgactcc gcaaccaaat ctaactacac
     3241 agcgggtaaa gttggcgtag aaaatctggt taatgcggtg ccgcaactaa aagacattgc
     3301 gaacgttaaa ggcgagcagg tagtgaatat cggctcccag gacatgaacg ataatgtctg
     3361 gctgacactg gcgaaaaaaa ttaacaccga ctgcgataaa accgacggct tcgtcattac
     3421 ccacggtacc gacacgatgg aagaaactgc ttacttcctc gacctgacgg tgaaatgcga
     3481 caaaccggtg gtgatggtcg gcgcaatgcg cccgtccacg tccatgagcg cagacggtcc
     3541 attcaacctg tataacgcgg tagtgaccgc agctgataaa gcctccgcta atcgtggcgt
     3601 gctggtggtg atgaacgaca ccgtactgga cggtcgcgat gtcaccaaaa ccaacaccac
     3661 cgacgtagcg accttcaagt ctgttaacta cggtcctctg ggatacattc acaacggtaa
     3721 gattgactac caacgtaccc cggcacgtaa gcacaccagc gatacgccat tcgatgtctc
     3781 taagctgaat gagctgccga aagtcggcat cgtttataac tacgctaacg catccgatct
     3841 tccggctaaa gcactggtag atgcgggcta tgatggcatc gttagcgctg gtgtgggtaa
     3901 tggtaacctg tataaatccg tgttcgacac cctggcaacc gccgcgaaaa acggcactgc
     3961 agtagtgcgt tcttcccgcg taccgacggg tgctaccact caggatgctg aagtggatga
     4021 tgcgaaatac ggcttcgtcg cctctggcac gctgaacccg caaaaagcgc gcgtcctgct
     4081 gcagctggct ctgacgcaaa ccaaagatcc gcagcagatc cagcagattt tcaatcagta
     4141 ctaatcacct cgtcccggca tcgtgccggg gctttttcac ttcagactta cgtccattgc
     4201 caattttcat taccctaatg ataatcaccg gaataaatta ttccgcgcga gggttttcgg
     4261 gtgaaaaagc aatggattgt tggcacggcg ctgcttatgt tgatgactgg taatgcctgg
     4321 gcagatggcg aaccgccaac tgaaaatatc ttaaaagatc aattcaaaaa gcagtatcac
     4381 ggcattctca agcttgatgc cat
//
LOCUS       DADLSB010000243         4354 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.243, whole genome shotgun sequence.
ACCESSION   DADLSB010000243 DADLSB010000000
VERSION     DADLSB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.243"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            105..257
                     /gene="hokA"
                     /locus_tag="JN331_003990"
     CDS             105..257
                     /gene="hokA"
                     /locus_tag="JN331_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY7261957.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(444..656)
                     /gene="cspA"
                     /locus_tag="JN331_003991"
     CDS             complement(444..656)
                     /gene="cspA"
                     /locus_tag="JN331_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY7261958.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(937..1227)
                     /locus_tag="JN331_003992"
     CDS             complement(937..1227)
                     /locus_tag="JN331_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY7261959.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1661..2371
                     /locus_tag="JN331_003993"
     CDS             1661..2371
                     /locus_tag="JN331_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY7261960.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="JN331_003994"
     CDS             complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="JN331_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY7261961.1"
                     /translation="MKPSVILYKALPDNLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3499..4158)
                     /locus_tag="JN331_003995"
     CDS             complement(3499..4158)
                     /locus_tag="JN331_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY7261962.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT         1071 a         1050 c         1059 g         1174 t
ORIGIN      
        1 taactgtcca actttttggg gtcagtacaa agtgaggcta ccctatgcct gaacaccata
       61 aggttagcct cttactcgtt ggaaatcaac acagggggct gggaatgccg cagaaatata
      121 gattactttc tttaatagtg atttgtttca cgcttttatt tttcacctgg atgataagag
      181 attcactgtg tgaattgcat attaaacagg agagttatga gctggcggcg tttttagcct
      241 gcaaattgaa agagtaagag tcttcggcgg gaaattattc ccgccttact tacggcgttg
      301 cgcattctca ttgcacccac atttattctt cacaaaaata ataatagatt ttattacgcg
      361 atcgattatt tatttcctga aaaaaaataa aaaaatcccc gccaaatggc agggatctta
      421 gattctgtgc ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta
      481 gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg
      541 ttctggatag cagagaagtg tacgaacaca tctttagagc catcgtcagg agtgatgaag
      601 ccgaagcctt tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg
      661 tattaccttt aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa
      721 gcgttaaagg aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt
      781 actacactgc tttaatggtc tgtacgtcaa accgttgatg tgcattaagc cacgcattgg
      841 cgggtgatgc aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc
      901 atcaggaccg taaacagcaa taaagtggat aaaagtctat ttcatcaact gcttacttaa
      961 tgccgggttg gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg
     1021 tctggattcc cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc
     1081 gatttttaat cctgtccctt ttcgtaactg ctcaatttcg gtacaggacg ttgttctgtg
     1141 cgtcagggta attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag
     1201 ctcatgcatt ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg
     1261 aattgcatcg aagctcatta agaatagtcg ggaaaagcag acacagcggg gtgaagtcag
     1321 ggcatgctct ggctatgatt cgattcagcc tgtcatttag cgtgacttat agccacctaa
     1381 cgatctgata aatgccgata tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc
     1441 taaccttctg cgaaggcgta aatctctggc tacaaaaaag gcaacggtac tatcattttt
     1501 taacaatgaa acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt
     1561 acgtagaaag ggtatcctgc aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt
     1621 gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg
     1681 ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt
     1741 tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg
     1801 tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt
     1861 ctctgattac gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg
     1921 tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca
     1981 aagcggtccg ctgcgtgaaa tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa
     2041 tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc
     2101 ggtatttgat caggcattca ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt
     2161 gattccggcg gcacaaactt tcacgcaaca actggtgatg gttggagact acattgctca
     2221 gcaaggtact caggtgagct ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc
     2281 aagtgaatat aacaaactga ttgcgccatt accggcacaa catcaggcgt ttaatcaggc
     2341 ctggactaca gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct
     2401 ttacttcacg cagtcgcggc ttagtccgcg acgtgaggat tcacacagtt cttctcaacc
     2461 tttccttgta acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc
     2521 tcatgggtgg cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc
     2581 gaatctacgg acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcgcct
     2641 ttctgcaatg ctgcgatcag tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg
     2701 aaaatggcgg aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca
     2761 tcagttaacg gcaggatcag gcaaacgaaa tctgactctt gtaacagagt atccaaatcg
     2821 cagtagcggg cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg
     2881 atgggcatgt tgaagccaaa gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc
     2941 ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc
     3001 gcggtccatt cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac
     3061 aacaccagcg ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc
     3121 agcagaattt ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc
     3181 gttgatgtgg cacgcagttt cggcattttt tccagcaatg cggcatctac attctcgttt
     3241 gaacccagta aaccttcagc ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg
     3301 ctgaggtttg ccacctggtg aacggtgaaa tgcgcttgca ggcgttgcag taaattatca
     3361 ggtaaggctt tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc
     3421 gatcacctta gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat
     3481 cacctggcat gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc
     3541 cttaccttct gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg
     3601 gatgcggctg gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgctg
     3661 ctgggagaga cgcatgttca ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt
     3721 aaccgccgtt ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc
     3781 tgccggtttc aggggcgcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat
     3841 aatgttatcc ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt
     3901 cgcttcctgc acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc
     3961 gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa gagagcgcac caatacccgc
     4021 gcccacgaga gagcccagac ctgcgccgat agcagatttg cctgcttcgc gttcgccggt
     4081 gtaagggtta gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaataag
     4141 ataaacacgt ttcttcattg ttaatcctta ataacctttt tattctttgc cacgggttcc
     4201 gtggcgggag attatgccgc gtgaacatga agattattcc tgggaatact cggaaatttg
     4261 taagtaatat ttaactgctc aatacatcta acctttcagg agtcttcggt ttggccaact
     4321 catcctcacg atattccgtt ctgactgccg ccca
//
LOCUS       DADLSB010000244         4345 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.244, whole genome shotgun sequence.
ACCESSION   DADLSB010000244 DADLSB010000000
VERSION     DADLSB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.244"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..2592
                     /locus_tag="JN331_003996"
     CDS             <1..2592
                     /locus_tag="JN331_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7261963.1"
                     /translation="RLAKEDIHFFDLKLLAFKTRGNRAATRHFGTKALVANQYDAIDV
                     TEFIKHMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEIGGIIFECEDEHYLAT
                     LDMQLNNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGKPFY
                     QRRLFFTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARP
                     LGTYEENGHVFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTD
                     GQSYFRGLEIKDYSTETITGLYDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLK
                     SAEDDAISQQEDLKVARDMLQSGIISFGKYHQTLIVSAPTSEQVIRDTNTLADTLKDL
                     GFIMTLSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHNFHPHKRDGNAWGE
                     AIAVVPTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQK
                     YRRPESFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTK
                     RNRNFLKKLMRMMCTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVLHE
                     APTKDARMNGLRIRLQQWAQGGEFGWVFDNDVDTFDIDNIDNFGIDGTEFLNDKDVRS
                     PLTFYLLYRVTSLLDGRRLVIFMDEFWKWLQDPEFSKFALDMLKVIRKLNGIFFPATQ
                     SPEEMCKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDIVTTLDPYARQM
                     VIMKSPLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKD
                     EMLRRAV"
     gene            2617..3327
                     /locus_tag="JN331_003997"
     CDS             2617..3327
                     /locus_tag="JN331_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB5"
                     /protein_id="HAY7261964.1"
                     /translation="MKTTLTALTVALCLFHVPAQAGIPVFDEVANASNIQQWAEKLQQ
                     WQQTVQHYQSQMNAYKQQLATATGIRDVQAFLNQAKNLTADIKNLKNRGISLNDLLTG
                     SGGSYAGELNRLYDKYKLFDNCPENASEAYRDSCKQMLLNQAVSVEDTSDIQEKISDT
                     LSDISNLASRIENAKDSKESQDLANVVSARSIQLNALTTQWEMSVKQSEMRNQLLAEQ
                     RRKAQAEQMRTAPIADLN"
     gene            3339..3623
                     /locus_tag="JN331_003998"
     CDS             3339..3623
                     /locus_tag="JN331_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HAY7261965.1"
                     /translation="MKLKTLTFIVLASVAALTGCEDKTSVDWYMAHHDDMLAKYRECI
                     FNHNFVPTDCQNARSALHREKAKPDVAEGYKQILKDLDKARVQEPIADLN"
     gene            3641..>4345
                     /locus_tag="JN331_003999"
     CDS             3641..>4345
                     /locus_tag="JN331_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAY7261966.1"
                     /translation="MSGIFVGLNSHIMEGFDSVLTSVAADTGGWMAHLFSIAVLSLYI
                     TWRAYQTMAGKLVRPFEEIVWDVSRMLIIMIFVTNAGGYLDLVSDAITGLRDGFSGDA
                     SIWARLDTVWEKAQALGEKLYSYDDSTYVALKGTLAQFLVWAGVMLLVGITAIINLIA
                     ELVMLLMLATAPIFIYCLMWSWFRDMFSNWLRIILGCILTSLFSGIALTVVMDYLDYI
                     LAGATKASDANYLTLAV"
BASE COUNT         1016 a         1227 c         1197 g          905 t
ORIGIN      
        1 cggctggcaa aagaggacat tcatttcttt gacctgaagt tgctggcgtt taaaacgcgt
       61 ggcaaccgcg cggcgacccg gcatttcggg acaaaggcgc ttgtcgcaaa ccagtacgac
      121 gccatagacg ttacggagtt tataaaacat atgcggctca acgagagtat cccgctgtcc
      181 cgctatctcc cctggtcttc ccacctgcac gagcaggtgg tgaagaacaa aaaaggtgac
      241 tttgtcagca cctgggagat cggcggtatc atttttgaat gcgaggatga acattacctc
      301 gccaccctgg acatgcagct caacaacgtt atccgctcct ttgaggggct acccgttacc
      361 ttctataccc atctggtgag cgacgaattt accgacacgc ttgaatgtga atccgggatt
      421 ccgttttcag atgaaatcgc gcgtctgttt tacgcctccc gcgagggcaa acctttctac
      481 cagcgtcgcc tcttcttcac cgtctgcttc aatccgcaca ccggcattga gcgggcgacc
      541 atgaaacaga agtccctggg cgaacgtaaa accctgctcg acaatgccct gcaggtgatg
      601 caggagcacc ggcaagcgat tggcacggcg ctgtcacgct acgcggcgcg gccgcttggc
      661 acttatgagg agaacgggca cgtgttctcc tcccagctgg cgttttacaa ctttctgatc
      721 accggcaggt ggcagaaagt ccgcgtcacc cgcacgccgt tttatgacgt gctgggcggc
      781 acggatattt tcccttccac cgacattggc caggtcagcc acaccgacgg ccagagctac
      841 tttcgcgggc tggaaatcaa ggattacagc actgaaacca tcaccgggct gtatgactcg
      901 cttctttatg cccgctgccg gtacgtgttc acgcagtcct atacctgcat gggtaaggat
      961 gaagcgcggg gctgcatccg caccatcgag aaacgcctga agtctgccga ggatgatgcg
     1021 atctcccagc aggaagacct gaaggtcgcc cgcgacatgc ttcagtccgg cattatttca
     1081 ttcgggaaat atcaccagac gcttattgtc agcgccccaa ccagcgagca ggttatccgg
     1141 gacaccaaca ccctggcgga taccctgaag gatctcggtt tcatcatgac cctgtcccgc
     1201 gagtcgctgt cggcggcgta tctcgcgcag ttgccgggtg tgtatcacct gcgcccgcgt
     1261 ctggtgccgg tgagcagcct caactacgcc gaaatggtgt cgtttcacaa ctttcacccg
     1321 cacaagcggg acgggaatgc ctggggggag gcgattgccg ttgttccgac cccgtccggg
     1381 ggatgtcagt acgtgaatct gcacaacagc caggccgatg ttgacgagtt cagcgagaag
     1441 ccgccgggta acaccatgct gctcggcagt accgggacgg ggaaatcgac cctgctgatg
     1501 atgttccagc atctgatgca gaaataccgg cgtcctgagt cgtttgcgcc gggggcgacg
     1561 aaaaagcgct tcacctcggt ggtctttgat aaagaccgtt cggcggaaat gtcgatacgc
     1621 ctccagggcg gctcgtactt ccgtttccgt tcaggcgaga agaccggcct taacccgttt
     1681 gcgctcgctc caacgaaacg taaccgcaac ttcctcaaga agctgatgcg aatgatgtgc
     1741 acaagggacg gcaaaccgct cgacccgcgc gatgaagaac gcctcagcga ggcagtggac
     1801 accatcatgc tggactaccc gcctgagctt cgtcgcaacg gcatcacccg catgctggaa
     1861 gtgctgcatg aagccccgac gaaggacgca cggatgaacg ggctgcgcat ccgtctccag
     1921 cagtgggcgc agggcgggga gtttggctgg gtgtttgata atgacgtgga cacctttgat
     1981 atcgacaata ttgataattt cggcatcgac ggcacggagt tcctgaacga caaagacgtg
     2041 cgcagcccgc tcaccttcta cctgctctac cgcgtgacca gcctcctcga tggccgccgc
     2101 ctggtcattt tcatggatga attctggaaa tggcttcagg acccggagtt ttcaaaattc
     2161 gccctcgaca tgctgaaggt cattcgaaag ctcaacggca ttttcttccc ggccacgcag
     2221 tcgccggagg agatgtgcaa gcacgagatc tcggcggcca ttatcgagca gtgtagtacc
     2281 cgcatcttca tggcgaacca gcaggcgaag gagtacgact acgtggacat gctgcgggtg
     2341 ccgaaagcgg tttacgacat cgtgaccacc ctcgacccct acgcccgcca gatggtgatc
     2401 atgaaatctc cgctgcggcg tggtgacgtg cgcccgtata tcgcacaaat caccctcgac
     2461 ctctccgggc taggtcgcta cacgaagctg ctcagcgcca gtgcggacaa cctgaaaatt
     2521 tttgacgaga tctggcagga gggtatgaag ccggaagact ggaaagacga aatgctgaga
     2581 cgtgccgttt aatcacaaaa ggataacacc atgaaactga aaaccaccct gacggcgctg
     2641 accgtcgcct tgtgtctctt tcacgtcccg gctcaggccg gtattcccgt ttttgatgaa
     2701 gtagccaacg ccagcaacat ccagcagtgg gcggagaagc tccagcagtg gcagcagacg
     2761 gttcaacatt atcagtcgca gatgaacgct tataaacaac agctggcaac ggccaccggt
     2821 attcgggacg tccaggcatt cctgaatcag gcaaaaaacc tgacggctga cattaaaaat
     2881 ctgaaaaaca gggggatttc cttaaacgat ctgttaaccg gctcaggcgg gtcttatgct
     2941 ggggaactaa acaggctgta tgacaaatac aaactgttcg acaactgccc ggagaacgcc
     3001 agcgaggcgt accgggatag ctgcaaacag atgctcctca atcaggcggt gtccgtcgag
     3061 gacacttctg acatccagga aaaaatttcc gacacgctga gcgatattag taatctcgcc
     3121 agtcgcatcg agaatgcgaa ggacagtaaa gaatcgcagg atctggcgaa cgtagtgtcc
     3181 gcgcggagta tccagctgaa cgccctgacc actcaatggg aaatgtcggt caaacaaagt
     3241 gagatgcgca accagctgct tgccgaacaa cgccgaaagg cgcaggccga gcaaatgcgc
     3301 acagctccaa tcgcagacct taactaacag gtgaccccat gaaactgaag acactgacct
     3361 ttattgttct ggcctcagtg gccgcgctta ctggctgcga ggataaaacc tccgtagact
     3421 ggtatatggc acaccatgac gacatgctgg cgaaatacag ggagtgcatt tttaatcata
     3481 attttgtgcc caccgattgc cagaatgcac gctccgccct tcaccgtgaa aaagctaaac
     3541 ctgatgttgc cgaaggatat aagcaaatcc tgaaagacct tgataaagct cgcgtgcaag
     3601 agcctattgc tgacctgaat tagcgacagg agaacgcacc atgagtggaa tattcgtcgg
     3661 attaaacagc cacatcatgg aggggtttga cagcgtgctc accagcgtgg ccgccgatac
     3721 gggggggtgg atggcacacc tgttctccat cgcggtgctc tcgctgtaca tcacgtggcg
     3781 cgcctaccag acgatggcag gcaagctagt ccgccccttc gaggagatcg tgtgggacgt
     3841 gagccggatg ctcatcatca tgattttcgt caccaacgcc ggaggctatc tcgatctggt
     3901 ttcggatgcc atcaccggcc tgcgggacgg attcagcggc gacgcgtcga tctgggcgcg
     3961 gctggatacc gtctgggaaa aggctcaagc acttggggag aagctttact cttatgatga
     4021 ttcaacctat gtcgctctaa aaggcactct ggcgcagttt ctggtctggg caggcgtcat
     4081 gttactggta ggcatcacgg ccatcattaa cctgatagcc gaactggtga tgctgctgat
     4141 gctggcgacc gccccgatct ttatctactg cctgatgtgg tcgtggttcc gggatatgtt
     4201 cagcaactgg ctcagaatta ttctgggctg catccttaca tccctgttca gcggcatcgc
     4261 cctgacggtg gtaatggatt acctggacta catcctggcg ggcgccacaa aagcatccga
     4321 tgccaactac ctgactctgg ccgtt
//
LOCUS       DADLSB010000245         4324 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.245, whole genome shotgun sequence.
ACCESSION   DADLSB010000245 DADLSB010000000
VERSION     DADLSB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.245"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            176..1459
                     /locus_tag="JN331_004000"
     CDS             176..1459
                     /locus_tag="JN331_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAY7261967.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            1606..3081
                     /locus_tag="JN331_004001"
     CDS             1606..3081
                     /locus_tag="JN331_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7261968.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            3360..>4324
                     /locus_tag="JN331_004002"
     CDS             3360..>4324
                     /locus_tag="JN331_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7261969.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVWMNFRLYQNVSRENISDAMTGLYNR"
BASE COUNT         1170 a          974 c          938 g         1242 t
ORIGIN      
        1 tacacccgta aacaggtgcg tttactgtgc gaatggtatt tgcgcgtacg taaatcgtct
       61 taacaaccct ggcccggtac acatgctacc gggcctacaa cgacagcgaa ccgtgggcct
      121 gagaagcggc aacacaggcg tagcatacag ttggcaaatg tagtacgggg ggcatatgac
      181 ctggtttatt gaccggcgtc tgaacggcaa aaacaaaagc atggtgaatc gccagcgttt
      241 tttacgccgt tataaagcgc aaattaaaca gtcgatctcc gaggccatta ataagcgttc
      301 ggtgactgac gtcgacagcg gcgaatccgt atccattccc acggaagata ttagcgaacc
      361 aatgtttcat caggggcgtg gcggtctgcg ccaccgcgtg catccgggca atgaccattt
      421 cgttcagaac gaccgaattg aacgtcccca gggtggcggc ggaggttccg gcagtggtca
      481 gggccaggcc agccaggatg gtgaaggtca ggatgaattt gtctttcaga tttcgaaaga
      541 tgagtatctt gatctgctct ttgaagattt ggccttaccg aatctgaaac aaaaccaaca
      601 acgccagctg accgaatata aaacgcatcg ggcgggttat accgctaacg gcgttccagc
      661 caatatcagc gttgtgcgtt ccttgcagaa ctcactggcg cgacgcacag ccatgacggc
      721 aggcaagcgg cgggaacttc atgcactgga agagaatttg gccatcatca gcaacagtga
      781 acctgcgcag ctgctggaag aggaacgtct gcgcaaagaa attgcagaat tacgtgccaa
      841 aattgaacgc gtccctttta ttgacacctt cgatttacgt tacaagaact acgagaagcg
      901 gcccgatccc tccagccagg cagtgatgtt ttgcctgatg gacgtttccg gttcaatgga
      961 tcaatccact aaagatatgg ctaagcgttt ttatattctg ctgtatctgt tcctcagcag
     1021 aacgtataag aacgtggaag tcgtatacat ccgccatcat acccaggcga aagaagtcga
     1081 tgaacatgag tttttctact cgcaggaaac aggcggcacc attgtttcca gcgccctgaa
     1141 actgatggat gaggtagtga aagagcgtta taacccggca cagtggaata tttacgctgc
     1201 acaagcatcg gacggcgata actgggccga tgactctccg ctttgccatg aaatcctggc
     1261 gaaaaaatta ttacctgttg ttcgttatta cagctatatc gaaattaccc gtcgtgcaca
     1321 tcagacattg tggcgagaat atgagcatct gcaatctact ttcgacaact ttgcgatgca
     1381 gcacatccgc gaccaggatg atatttatcc ggtgttccgt gaactgtttc ataaacaaaa
     1441 tgcaacagct aaagactaaa actatcagcc gggtcattat cgcctggctg atttttagct
     1501 tactgtaaat tatctcattt attacataca ttagcttaca atcgctttaa atatgacagc
     1561 ataaccttta cacaatttag ttccagaaaa caatcattcg gaaaaatgat tcagtcaaca
     1621 cgtatttccc tggggttatt ctttaaatat tttttatcgt taacgaaaat tgatcttggt
     1681 caaaactata tatctctgcc atcaataaaa tccagcactc acattgctct cctttttatg
     1741 gtttctatgg gtacacaaaa attaaaagct caaagctttt ttattttcag tttattgctg
     1801 acgttaattt tattttgcat caccacatta tataacgaaa agacgaatgt aaaactcatc
     1861 ccacaaatga attatctgat ggtcgttgtg gctttgtttt tccttaacgc catcattttt
     1921 cttttcatgt taatgaaata tttcactaac aaacaaattc taccaacact cattttaagt
     1981 cttgcatttt taagtggcct tatctattta gttgaaacta ttgttgttat ccataaacct
     2041 atcagcgaca gtacactgat ccagacaaag tcgaatgatg tttctatttt ctatattttc
     2101 cgccaactca gttttatttg tttaacctcg ttggcgctct tttgttatgg taaagacaac
     2161 atccttgaca acaataagaa aaaaactggc attctgttgc tggcgctgat tcctttttta
     2221 gtttttcccc ttctggcaca caatctgagc agttataacg ctgactattc tttgtatgtc
     2281 gtcgattact gtcctgacaa ccatactgcg acctggggaa tcaactatac aaaaatattg
     2341 gtttgtctgt gggcattttt actgttcttt attatcatgc gtacacgatt agccagcgaa
     2401 ctatggccat taatagcatt attatgtctg gcatcgctat gctgcaactt acttctactg
     2461 actctggatg agtataatta caccatctgg tacatcagtc gcgggattga agtctccagt
     2521 aaactgtttg ttgtgtcttt tctgatttat aacatttttc aggaactgca actctccagc
     2581 aaactggcag ttcatgatgt gctgaccaat atttataatc ggcgctactt tttcaacagc
     2641 gtagagtcat tattgtcgcg acctgttgtt aaggacttct gtgtcatgct ggttgatatt
     2701 aatcagttca aacgcatcaa tgcccaatgg ggacatcgtg tgggtgataa agtgctggtt
     2761 tcaattgtcg atattatcca gcaaagcatc cgccccgatg atattttagc gcgactggag
     2821 ggtgaggtgt ttggcttgct atttaccgaa ctcaatagtg cccaggcaaa aatcattgcg
     2881 gaacgtatgc gtaaaaatgt cgaactcctg accggcttca gtaacagata tgatgttcct
     2941 gaacaaatga ccatcagtat tggcacggtt ttttcaacgg gtgacacgcg taatatctcg
     3001 cttgtcatga cggaagcaga taaagcctta cgcgaagcga aaagcgaggg gggcaacaaa
     3061 gtgattattc ataatattta agtgcaaaaa tattcagagc catgcttttt gcgtggcttt
     3121 tgcatacaat ttattactat tacccctaaa tttcccctcc ccagcggcgc agagatgagt
     3181 ataattagcg cccctgtgcc aggccgcaat cgaactttat ctggttttct cgtttcacta
     3241 accgaaggag tgccatttat catgaaattg caccatagaa tgctccggca ttttatcgcc
     3301 gcaagtgtca ttgtgctgac atcttcctta tttttgaact tgtcgccagc gacagagcaa
     3361 tgagtgccta tctgcgctat atcgtgcaga aagcagactc ctcctttctt tatgataagt
     3421 atcagaatca gagtattgcc gcgcatgtga tgcgcgctct cgctgctgag cagtcggaag
     3481 tgtcgccaga acagcggcgc gccatctgcg aggcttttga gtctgccaat aatacccatg
     3541 gcttaaacct gactgcccat aaatacccgg gcttacgcgg cacactacaa accgcatcca
     3601 ctgactgcga cacaattgtg gaagctgcag cactattacc cgcttttgat caggcagtgg
     3661 aaggcaaccg ccaccaggat gattacggtt caggtcttgg gatggccgaa gagaaatttc
     3721 actattatct cgatctcaat gaccgctatg tctattttta tgagccggtt aatgttgaat
     3781 actttgcgat gaataactgg tccttcctgc agtcaggaag tattggcatc gatcgcaaag
     3841 atattgaaaa ggtatttacc gggcgtaccg tattgtcgag catttaccag gatcagcgta
     3901 ctaaacagaa cgtgatgagt ttgctgacgc cggtatatgt cgcagggcag ctaaaaggga
     3961 ttgtgctgct ggatattaac aaaaacaatt tgcggaatat cttttacact catgaccgcc
     4021 ctctcctctg gcgttttctc aatgtcacgc taaccgatac cgattcgggg cgcgacatta
     4081 tcatcaacca gagcgaagat aatctgttcc agtatgtcag ttacgtccat gacttaccgg
     4141 gcggcattcg tgtctcgtta tccattgata ttctttactt tatcacgtct tcgtggaaaa
     4201 gcgttctgtt ctggattttg acggcgttaa ttttgctgaa tatggtctgg atgaacttcc
     4261 gtttatacca aaatgtgtcg cgagaaaata ttagtgatgc gatgactgga ctgtataatc
     4321 gcaa
//
LOCUS       DADLSB010000246         4313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.246, whole genome shotgun sequence.
ACCESSION   DADLSB010000246 DADLSB010000000
VERSION     DADLSB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.246"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..767
                     /gene="cheR"
                     /locus_tag="JN331_004003"
     CDS             <1..767
                     /gene="cheR"
                     /locus_tag="JN331_004003"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAY7261970.1"
                     /translation="LIYQRAGIVLADHKRDMVYNRLVRRLRSLGLTDFGHYLNLLESN
                     QHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYRVWSAAASTGEEPYSIA
                     MTLADTLGTVPGRWKVFASDIDTEVLEKARSGIYRHEELKNLTPQQLQRYFMRGTGPH
                     EGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMIYFDQTTQQEILRRFVP
                     LLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            770..1819
                     /locus_tag="JN331_004004"
     CDS             770..1819
                     /locus_tag="JN331_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAY7261971.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            1834..2223
                     /gene="cheY"
                     /locus_tag="JN331_004005"
     CDS             1834..2223
                     /gene="cheY"
                     /locus_tag="JN331_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAY7261972.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDSLN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            2234..2878
                     /gene="cheZ"
                     /locus_tag="JN331_004006"
     CDS             2234..2878
                     /gene="cheZ"
                     /locus_tag="JN331_004006"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAY7261973.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQASERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(3335..3718)
                     /locus_tag="JN331_004007"
                     /pseudo
     CDS             complement(3335..3718)
                     /locus_tag="JN331_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3687..>4313)
                     /locus_tag="JN331_004008"
     CDS             complement(3687..>4313)
                     /locus_tag="JN331_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HAY7261974.1"
                     /translation="EAKNDTWKEQRNALSPAEINELSMQMFRVYATSALINAVVEAFC
                     EQLPALMQRDCPFKDLISQSKGHALCTALKMFDRTRGYLHRSVLQQELRGSNYITNLM
                     DMLWIGIHGHKTKKQRDGERESASADNLFVSREEYLSDTPFGRYAYGTISENYRRVFE
                     DPANTLPVLYKEAQLLTDAISGMTDSYLMRLHDELKSLYECGLKHPTA"
BASE COUNT         1082 a          981 c         1183 g         1067 t
ORIGIN      
        1 aattgatcta tcaacgagcc gggatcgttc tggctgacca taaacgcgac atggtttaca
       61 accgactggt tcgtcgtttg cgttcgctgg gactgacgga tttcggtcat tatctgaact
      121 tgctggaatc taatcagcac agcggtgagt ggcaggcgtt tatcaattcg ctgaccacga
      181 atctgacggc atttttccgt gaggctcatc atttccctct gctcgcggat cacgcacgtc
      241 gccgttctgg cgagtatcgc gtatggagcg cggcggcttc gaccggcgaa gagccgtaca
      301 gcattgcgat gactctggct gacacattgg gcaccgtgcc cggacgctgg aaagtgtttg
      361 ccagtgatat cgacaccgaa gtgctggaaa aagccagaag cggtatctat cgccatgaag
      421 agttgaaaaa cctgacgccg cagcaactgc aacggtattt catgcgaggg acgggaccgc
      481 atgaagggct ggtacgcgtg cgtcaggagc tggcgaacta tgttgatttt gccccgctga
      541 atttactggc gaaacagtac accgtgccgg ggccgtttga tgcgatcttc tgtcgtaacg
      601 tcatgatcta cttcgatcaa actacccagc aggagatttt gcgccgcttt gttccgctcc
      661 ttaaacccga cggattgctg tttgcgggtc actctgaaaa ctttagccac ctcgagcgcc
      721 gcttcacgct gcgtggtcag acggtgtatg cgctaagtaa ggattaacga tgagcaaaat
      781 cagggtgtta tctgtcgatg attcggcact gatgcgccag atcatgacag aaatcatcaa
      841 cagccatagc gacatggaaa tggtggcgac cgcgcctgat ccgctggttg cgcgtgactt
      901 gattaagaaa ttcaatcccg atgtgctgac gctggatgtt gaaatgccgc ggatggacgg
      961 actggatttc ctcgaaaaat taatgcgttt gcgtccaatg cccgttgtga tggtttcttc
     1021 cttgaccggc aaaggatcag aagtcacgct gcgcgcgctg gagctggggg caatagattt
     1081 tgtcaccaaa ccgcaactgg gtattcgcga aggaatgctg gcgtatagcg aaatgattgc
     1141 tgaaaaggtg cgtacggcag caaaggcgag ccttgcagca cataagccat tgtcagcacc
     1201 gacaacgctg aaggcagggc cgttgttgag ttctgaaaaa ctgattgcga ttggtgcttc
     1261 aacgggtgga actgaggcaa ttcgtcacgt actgcaaccg ttgccgcttt ccagcccggc
     1321 attgttaatt acccaacata tgccgcccgg tttcacccgc tcttttgccg acagacttaa
     1381 taagctttgc cagatcgggg ttaaagaagc cgaagacgga gaacgtgtct tgccggggca
     1441 tgcctatatt gcgccgggcg atcggcatat ggaactggcg cgtagtggcg caaattatca
     1501 aatcaaaatt cacgacggcc cggcggttaa ccgtcatcgg ccttcggtag atgtgttgtt
     1561 ccattctgtc gccaaacagg cggggcgtaa tgcggttggg gtgatcctga ccggtatggg
     1621 caacgacggc gcggcgggaa tgttggcgat gcgtcaggcg ggggcatgga cccttgcgca
     1681 aaacgaagca agttgcgtgg tgttcggcat gccgcgcgag gccatcaata tgggtggtgt
     1741 ctgcgaagtg gtcgatctta gccaggtaag ccagcaaatg ttggcaacaa ttagtgccgg
     1801 acaggcgata cgtatttaaa tcaggagtgt gaaatggcgg ataaagaact taaatttttg
     1861 gttgtggatg acttttccac catgcgacgc atagtgcgta acctgctgaa agagctggga
     1921 ttcaataatg ttgaggaagc ggaagatggt gtcgactctc tcaataagtt gcaggcaggc
     1981 ggttatggat ttgttatctc cgactggaac atgcccaata tggatggcct ggaattgctg
     2041 aaaacaattc gtgcggatgg cgcgatgtcg gcattgccag tgttaatggt gactgcagaa
     2101 gcgaagaaag agaacatcat tgctgcggcg caagcggggg ccagtggcta tgtggtgaag
     2161 ccatttaccg ccgcgacgct ggaggaaaaa ctcaacaaaa tctttgagaa actgggcatg
     2221 tgaggatgcg actatgatgc aaccatcaat caaacctgct gacgagcatt cagctggcga
     2281 tatcattgcg cgcatcggca gcctgacgcg tatgctgcgc gacagtttgc gggaactggg
     2341 gctggatcag gccattgccg aagcggcgga agccatcccc gatgcgcgcg atcgtttgta
     2401 ctatgttgtg cagatgaccg cccaggcttc ggagcgggcg ctgaacagtg ttgaggcgtc
     2461 acaaccgcat caggatcaaa tggagaaatc agcaaaagcg ttaacccaac gttgggatga
     2521 ctggtttgcc gatccgattg accttgccga cgcccgtgaa ctggtaacag atacacgaca
     2581 atttctggca gatgtacccg cgcataccag ctttactaac gcgcaactgc tggaaatcat
     2641 gatggcgcag gattttcagg atctcaccgg gcaggttatt aagcggatga tggatgtcat
     2701 tcaggagatc gaacgccagt tgctgatggt gctgttggaa aacatcccgg aacaggagtc
     2761 gcgtccaaaa cgtgaaaacc agagtttgct taatggacct caggtcgata ccagcaaagc
     2821 cggcgtggta gccagtcagg atcaggtgga cgatttgttg gatagtcttg gattttgatt
     2881 tgtattgcct gaacttcggc ctgcgcgatg atgccaggcc gatagcaggg tggcgggtat
     2941 cgttcaggga gtacgtggag gatggttatt tgtagtttga taattttaga tcagcgaagc
     3001 gtgattgttg cgccagtaaa tttatcaatg cattaaattg tgaacgagaa aatgacgatg
     3061 actggtagtg cagtacatcg tagtgatgcc atacttcata acgttttttc gtcagttcat
     3121 ggtgcaagaa atgacagagt tccggcccta aactctttac ctgcttagta aaaatagtgc
     3181 gtaaaatctt cgttaccgaa ccgcttggat tggggttagt atgaagaaca agttcaagat
     3241 gtctgcgatt taagtcggtc aagtaattat ctttactgat aattttttca ctacgtagat
     3301 catagagcac ggcatcccaa ctcattagcg tagtgtttaa cagacttgat tcatcctcac
     3361 aaaatataag tttttcccgg aatgcccatg tctcattgag gttccagata tcaaattcga
     3421 agtcgtgata acgaaaacgg atcccaccaa acttattaaa acgcagttgt ttgacgggca
     3481 gttcagccaa tgtctggaag agttcctcac gggagcgacc gatgacgata tcaaggtctg
     3541 aagagaatcc atcgacgcca tacaagccta agtcacgcac catcccgcca aaaatccagg
     3601 ccggacagga acaggccaga gtatcaagta atactctggc tttcttatgc tcatcactaa
     3661 gcgtaaaaaa atcggcgata cgctgttcaa gctgttgggt gtttgagacc acattcatac
     3721 agtgatttta gctcatcgtg aaggcgcatt aaataactat ccgtcatgcc tgagattgcg
     3781 tcggtaagca actgcgcttc tttataaaga acagggagtg tattggcggg atcttcaaac
     3841 acgcggcgat aattttcaga tattgtcccg taagcatagc gaccaaaggg agtatccgac
     3901 aggtattctt cacgagaaac aaaaaggtta tctgcagacg cggactcgcg ttctccgtct
     3961 cgttgtttct ttgttttgtg tccatgaatg ccaatccaga gcatatccat caagttagta
     4021 atgtaattag accccctaag ttcctgttgc aagactgaac gatgaaggta gcctcgtgtt
     4081 cggtcgaaca ttttcagggc agtgcagagg gcgtgacctt tgctttgcga gattaaatct
     4141 ttgaacgggc agtcacgctg catcagcgcg ggtaattgtt cgcaaaaggc ttctactacg
     4201 gcattaatca atgcactggt ggcatacact ctgaacattt gcatactgag ttcattaatt
     4261 tccgccggtg aaagtgcatt tctttgctct ttccaggtgt catttttggc ctc
//
LOCUS       DADLSB010000247         4313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.247, whole genome shotgun sequence.
ACCESSION   DADLSB010000247 DADLSB010000000
VERSION     DADLSB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.247"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..532)
                     /locus_tag="JN331_004009"
     CDS             complement(<1..532)
                     /locus_tag="JN331_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261975.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVS
                     ENTKQRNLFSKKMTLCI"
     gene            1047..3470
                     /gene="pgaA"
                     /locus_tag="JN331_004010"
                     /pseudo
     CDS             1047..3470
                     /gene="pgaA"
                     /locus_tag="JN331_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            3479..>4313
                     /gene="pgaB"
                     /locus_tag="JN331_004011"
     CDS             3479..>4313
                     /gene="pgaB"
                     /locus_tag="JN331_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HAY7261976.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GL"
BASE COUNT         1271 a          950 c          946 g         1146 t
ORIGIN      
        1 ttatgcacaa agtcattttt ttggaaaata aattgcgctg ttttgtattt tcactaactt
       61 tattcaccaa tgccagaaat attaatatgc acaaactcat ctgacgaaac aaataataga
      121 ttgcaatgtc attttggaca acactgcttg tcaaacgccc ctcaataatt tgctgaatga
      181 taatgacggt tttaacaaag taaatcaaac aactcagaaa agccaaacta agtatacagt
      241 ttgaaaattg cctacggtca taaatgaaat acaactgcat aaaaataaat gcaacagtat
      301 cgataaaaaa taaagcaatc attaccatca gataggttga tcgtggaaca acttcctcaa
      361 cgcctggctg attaaaccaa ctattcacca aaaccaaagc aagcccaaac aataagctca
      421 ctaatacagt actactaatt ctcaaatagt ctttttccat aaagctacac caatctgtag
      481 gttgtagatc cctttctatt attaatagat ataagaactg tactgttgtc aatatgaaat
      541 tccgcccaca aacaataccg atgatgatag ttgctgaatg taattattat tgcctaagtg
      601 ctttatacat tgtttgcaac aagccaagct tgaaaatact tataaatggt tatagaaaat
      661 ccttataaaa cattaattca tagatacatt aggaataaca attaaatccg tgagtgccgt
      721 agcgcagcct ttcatcagga ctttcgaaaa atccgaaatc atgcatcgga atttactgat
      781 ttaattattt taatcctaat ttattttgaa aaaggcattg ggatttatgc cgtattcctg
      841 aagatcctca tcattggaat ggattttcgg gcgagaaaag gattttatat ggacactctg
      901 ctcatcattt cttcttctca tcatcaacaa ttcacgtctc tcttccgcgt ttaataacgg
      961 attatgaggt gcaaaaatat ctttcttttc agttacctgt aattagatac agagagagat
     1021 tttggcaata catggagtaa tacaggatgt attcaagtag cagaaaaagg tgcccgaaaa
     1081 ccaaatgggc tttgaaactt cttactgccg catttttagc agcgagtccc gcggcgaaga
     1141 gtgctgttaa taacgcctat gatgcattga ttattgaagc tcgcaagggt aatactcagc
     1201 cagctttgtc atggtttgca ctaaaatcag cactcagcaa taaccaaatt gctgactggt
     1261 tacagattgc cttatgggcc gggcaagata aacaggttat taccgtttac aaccgctacc
     1321 gtcctcagca attaccagcg cgtggttatg cagctgtcgc cgtcgcttat cgtaacctgc
     1381 aacaatggca aaactcgctt acactgtggc aaaaggcgct ctctctggag ccgcaaaata
     1441 aggattatca acggggacaa attttaaccc tggcagatgc gggtcactat gatactgcgc
     1501 tggttaaact taagcagctt aactctggag caccggacaa agccaattta ctcgcagaag
     1561 cctatatcta taaactggcg gggcgtcatc aggatgaatt acgggcgatg acagagtcat
     1621 tacctgaaaa tgcatctacg caacaatatc ccacagaata cgtgcaggca ttacgtaata
     1681 atcaacttgc tgccgcgatt gacgatgcca atttaacgcc agatattcgc gctgatattc
     1741 atgccgaact ggtcagactg tcgtttatgc ctacgcgcag tgaaagtgaa cgttatgcca
     1801 ttgccgatcg cgccctcgcc caatacgctg cattagaaat tctgtggcac gataacccag
     1861 accgcactgc ccagtaccag cgtattcagg ttgatcatct tggcgcgtta ttaactcgcg
     1921 atcgttataa agacgttatt tctcactatc agcgattaaa aaagacgggg caaattattc
     1981 cgccctaggg gcaatattgg gttgcatcgg cttatctcaa agatcatcag ccgaaaaaag
     2041 cacagtcaat aatgaccgag ctcttttatc acaaggagac cattgccccg gatttatccg
     2101 atgaagaact tgcggatctc ttttacagcc acctggagag tgaaaattat ccgggcgcgc
     2161 taactgtcac ccaacatacc attaatactt cgccgccttt ccttcggtta atgggcacgc
     2221 ctacgagcat cccgaatgat acctggttac aggggcattc gtttctctca accgtagcaa
     2281 aatatagtaa tgatcttcct caggctgaaa tgacagccag agagcttgct tataacgcac
     2341 caggaaatca gggactgcgc attgattacg cgagtgtgtt acaagcccgc ggttggcctc
     2401 gtgcagcaga aaatgaatta aaaaaagcag aagtgatcga gccacgtaat attaatctgg
     2461 aggttgaaca agcctagaca gcattaacgt tacaagaatg gcagcaggca gctgtcttaa
     2521 cgcacgatgt tgtcgaacgt gaaccgcaag atcccggcgt tgtacgttta aaacgtgcgg
     2581 ttgatgtaca taatcttgca gagcttcgta tagctggttc aacaggaatt gatgccgaag
     2641 gcccggacag tggtaaacat gatgtcgact taaccaccat cgtttattca ccaccgctga
     2701 aggataactg gcgcggtttt gctggattcg gttatgccga tggacaattt agcgaaggaa
     2761 aagggattgt tcgcgactgg ctggcgggtg ttgagtggcg gtcacgtaat atctggctcg
     2821 aggcggagta cgctgaacgc tttttcaatc atgagcataa acccggcgcg cgcctgtctg
     2881 gctggtatga ttttaatgat aactggcgta ttggttcgca actggaacgc ctctctcacc
     2941 gcgttccatt acgggcaatg aaaaatggtg ttacaggcaa cagtgctcag gcttatgttc
     3001 gctggtatca aaatgagcgg cgtaagtacg gtgtctcctg tgctttcact gatttttccg
     3061 acagtaacca gcgtcatgaa gtctcaattg agggtcagga acgcatctgg tcttcaccat
     3121 atttgattgt cgatttccta cccagtctgt attacgaaca aaatacagaa cacgataccc
     3181 catactacaa ccctataaaa acgttcgata ttgttccggc atttgaggca agtcatttgt
     3241 tatggcgaag ctatgaaaat agctgggagc aaatcttcag cgcaggtgtt ggtgcctcct
     3301 ggcaaaaaca ttatggcacg gatgtcgtca cccaactcgg ctacgggcaa cgcattagct
     3361 ggaatgacgt gattgatgct ggcgcaacgc tacgctggga aaaacgacct tatgacggtg
     3421 acagagaaca caacttatac gttgaattcg atatgacatt cagattttaa ggataaatat
     3481 gttacgtaat ggaaataaat atctcctgat gctggtgagt ataattatgc tcaccgcgtg
     3541 catcagccag tcaagaacat catttatacc gccacaggat cgcgaatctt tactcgccga
     3601 gcaaccgtgg ccgcataatg gttttgtggc gatttcatgg cataacgttg aagacgaagc
     3661 tgccgaccag cgttttatgt cagtgcggac atcagcactg cgtgaacaat ttgcctggct
     3721 gcgcgagaac ggttatcaac cggtcagtat tgctcaaatt cgtgaagcac atcgaggagg
     3781 aaaaccgcta ccggaaaaag cagtagtgct gacttttgat gacggctacc agagttttta
     3841 tacccgcgtc ttcccaattc ttcaggcctt ccagtggcct gctgtatggg cccccgtcgg
     3901 cagttgggtc gatacgccag tggatgaaca agtaaagttt ggcgatgaga tggtcgatcg
     3961 agaatatttt gccacgtggc aacaagtgcg agaagttgcg cgttcccggc tcgttgaggt
     4021 cgcttctcat acatggaatt ctcactacgg tattcaggct aattccaccg gcagcttaat
     4081 gcctgtatat gtaaatcgtg catattttac tgaccacgca cggtatgaaa ccgcagcaga
     4141 ataccgggaa agaattcgtc tggatgctgt aaaaatgacg gaatacctgc gtacaaaggc
     4201 tgaggtaaat ccacacgttt ttgtttggcc ttatggcgaa gcgaatggca tagcgataga
     4261 ggaattaaaa aaactcggtt atgacatgtt tttcaccctt gaatcaggtt tgg
//
LOCUS       DADLSB010000248         4261 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.248, whole genome shotgun sequence.
ACCESSION   DADLSB010000248 DADLSB010000000
VERSION     DADLSB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.248"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..831
                     /locus_tag="JN331_004012"
     CDS             <1..831
                     /locus_tag="JN331_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY7261977.1"
                     /translation="QPTQLLNFDPSTMQWATSSPVPSTFIGALIISIVAGYLVKWMNQ
                     KIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITPFGGWINGGIRTVLTAAGEKGALM
                     YAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLPVTARSIAIVIPPIGLGLATIIDR
                     RLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFALESPITAIPSYMVGAIVGSTAAV
                     WLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAIITALMVVFLRLMMFRKGKLLIDSL
                     "
     gene            846..1929
                     /gene="ypdF"
                     /locus_tag="JN331_004013"
                     /pseudo
     CDS             846..1929
                     /gene="ypdF"
                     /locus_tag="JN331_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            1929..2965
                     /gene="ypdE"
                     /locus_tag="JN331_004014"
                     /pseudo
     CDS             1929..2965
                     /gene="ypdE"
                     /locus_tag="JN331_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            2990..>4261
                     /locus_tag="JN331_004015"
     CDS             2990..>4261
                     /locus_tag="JN331_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7261978.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQSTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGVPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFE"
BASE COUNT          912 a         1137 c         1263 g          949 t
ORIGIN      
        1 cagccgacgc aactgctgaa cttcgatccc agcactatgc aatgggcgac ctcttcgccg
       61 gtgccgtcca ccttcattgg tgcgctgatt atttctatcg ttgccggtta ccttgtgaaa
      121 tggatgaacc agaaaatcca gttaccggat ttcctgttag cgttcaaaac cacatttttg
      181 ctaccgattc tttccgccat ttttgtcatg ctggcgatgt actacgtcat cacccccttt
      241 ggtggctgga tcaacggcgg tatccgtact gtgctgactg ccgcaggtga gaagggcgcg
      301 ctgatgtacg caatggggat cgccgcggcg acagcaatcg accttggtgg cccgatcaac
      361 aaagccgcag gtttcgttgc cttcagcttt accactgacc acgtattacc ggtcaccgct
      421 cgttctatcg ctatcgttat tccgccgatt ggtctgggtc tggcgaccat tattgaccgt
      481 cgtttaaccg gcaaacgcct gttcaacgct caactttatc cgcagggtaa gaccgcgatg
      541 ttcctggcct ttatggggat tagtgagggt gcgattccgt ttgcgctgga aagccccatc
      601 accgccattc cgtcatatat ggtcggcgcg attgtcggct caaccgcagc tgtctggctg
      661 ggcgcagtgc aatggttccc ggaatccgct atctgggcat ggccgctggt cactaacctg
      721 ggcgtttata tggcggggat cgcgctgggg gcaatcatta ctgcgctgat ggtggtgttc
      781 ctgcgtctga tgatgttccg caaaggcaaa ttgttaatcg atagcctgta agaaggaaaa
      841 tccagatgac attactcgct tcgctgcgcg actggcttaa ggtgcaacaa ctggatgcgg
      901 tgcttctctc ctcacggcag aacaaacagc cgcatctggg gatctccact ggatcaggct
      961 atgtggttat tagccgtgaa agtgcgcaca ttctggtaga ttcgcgctat ttcgttgagg
     1021 tggaagcccg tgcgcaaggc taccagctgc atttgcttga cgcgacgaac acgcttacca
     1081 ctatcgtcaa tcaaatcatt gccgatgagc agttgcaaac gctcggtttt gaaggccagc
     1141 aggtgagttg ggaaaccgcg caccgctgga agtctgaact caatgcgaaa ctggttagcg
     1201 ccacgccgga tgtgctgcgg caaatcaaaa cgccagagga ggtggagata atccgcctcg
     1261 cctgtgggat tgccgatcgc ggagcggagc atattcgccg ctttattcag gcggggatga
     1321 gcgagcgcga gatagccgct gaactggagt ggtttatgcg ccagcagggc gcagaaaaag
     1381 cctcttttga caccattgtc gccagcggct ggcgcggggc gctgccgcac ggcaaagcca
     1441 gcgacaagat tgttgcagcg ggcgagtttg tcactctcga ttttggtgcg ctgtatcagg
     1501 gctactgctg atatgacgcg caccttgctg gtgaatggcg aaggggtgag cgccgaatct
     1561 cacccgctgt ttgacgtcta tcagattgtc ctgcaggcac agctcgcggc aatctctgca
     1621 attcgccccg gcgtgcgctg ccagcagatt gacgacgccg cgcgccgggt cattaccgag
     1681 gctggatttg gcgactattt cggtcataac actggtcacg ctatcggcat tgaagtccat
     1741 gaagatccgc gtttttcacc gcgggacacc acgacgctac agccaggcat gttgctgacc
     1801 gtggagccgg ggatttattt gctagggcaa gggggcgtgc gcatcgaaga tgttgtgctg
     1861 gttaccccgc aaggcgcaga agtgctctac gccatgccga aaacagtgtt gctcacggga
     1921 gaggcataat ggatttatcg ctattaaaag cgttgagcga ggcagatgcg atcgcctcct
     1981 cggaacagga agtgcggcag atcctgctgg aagaagcggc tcgcctgcaa aaagaagtgc
     2041 gatttgatgg tctgggatcg gtgctgatcc gcctcaatga atcgacaggt ccgaaggtga
     2101 tgatctgcgc ccatatggac gaagtgggat ttatggtgcg cagcatctcc cgcgaaggcg
     2161 ctattgacgt gctgccggtt ggcaacgtgc gcatggctgc ccgccagctg cagccggtgc
     2221 gcatcaccac ccgtgaagag cgcaaaattc caggcctgct tgacggcgaa cggcagggga
     2281 atgacgttag cgccatgcgc gtggacattg gcgcgcgcac ctatgacgaa gtgatgcagg
     2341 cgggaattcg tccaggcgat cgcgtcacct tcgataccac ttttcaggtc ctccctcacc
     2401 agcgggtgat gggcaagcct ttgatgatcg cctcggttgc tatctgctgg tgacgttact
     2461 acgtgagctg cacgacgccg aactgcctgc ggaagtgtgg ctggtggcaa gttccagcga
     2521 agaggtggga ttacgcggcg ggcaaactgc tacccgcgcg gtgtcgccgg acgtcgccat
     2581 tgtgcttgat accgcctgct gggcgaaaaa ctttgattat ggcgcggcta accatcgtca
     2641 gattggtaac gggctgatgc tggtgttaag cgataaatcg ctgattgcgc cgccaaaact
     2701 taccgcgtgg attgaaaccg tggcggcaga aattggcgtt ccgttgcagg cggatatgtt
     2761 cagtaacggc ggcacggacg gtggagcggt gcacttaacc ggtactggcg tacccacagt
     2821 ggtaatgggg cctgccaccc gccacggaca ttgcgcggca tcgattgccg attgccgcga
     2881 cattttgcag atggagcaac ttttatctgc ccttattcaa cgtcttacgc gtgagacggt
     2941 tgttcaactg acggatttca gatgatctcc tgattaaccc ggagcggtta tgttaacgat
     3001 tcaatttctc tgtcctttgc ctaacggtct acatgctcgt ccggcgtggg aacttaaaga
     3061 acagtgcagc cagtggcaaa gcgaaatcac ctttatcaac catcgccaga acgcaaaggc
     3121 cgatgctaaa agctcgctgg cgttgattgg aactggcacc ttatttaatg acagttgcag
     3181 cctgaacatt agcggcagcg atgaagagca ggcgcggcgc gtgctggaag agtacatcct
     3241 ggtgcgcttt atcgacagcg acagcgttca gtcgacgcag gcagaactga cggcgcatcc
     3301 gctgccgcgt tcattaagcc gccttaaccc ggatttactg tacggcaatg tgctggcaag
     3361 cggcgtcggc gtgggtacgc tgaccctgtt acaaagcgac agcctcgaca gttatcgggc
     3421 aatccccgcc agtgcgcaag attccacccg gctggagcac agcctggcaa cgcttgccga
     3481 gcaactgaac cagcaattgc gtgagcgtga cggcgaaagc aaaactatcc tcagcgctca
     3541 tttgtcgctg attcaggatg atgaatttgc aggcaatatc cgtcgcctga tgacagaaca
     3601 gcatcaggga ctgggggcgg cgatcatcag caatatggag caggtttgcg ccaaactatc
     3661 tgcttctgcc agcgattatc tgcgcgagcg ggtcagcgac attcgcgata tcagcgaaca
     3721 gttgctgcat atcacctggc cggaactgaa gccgcgcaac aatctggtgc tggaaaaacc
     3781 gaccattctg gtggctgaag atttaacccc aagccagttt ttgagccttg atttgaaaaa
     3841 tcttgcgggt atgattctgg agaaaaccgg gcgcacctcg catacactga ttctggcccg
     3901 tgcctcggcg atcccggtac tgagtggcgt gccgctggat gcgattgccc gttatgccgg
     3961 gcaacctgca gtgcttgacg cccagtgcgg cgtgctggcg attaacccga atgacgcggt
     4021 gagcggttat tatcaggtcg cgcagacgct ggcggataaa cgccaaaaac aacaggcgca
     4081 ggctgccgcg cagctggcct attcccgtga taacaagcgt attgatattg cggcgaatat
     4141 cggcaccgct ctggaagcgc caggcgcgtt tgccaacggc gcggaaggtg tcgggctgtt
     4201 ccgtaccgaa atgctctata tggatcgcga cagcgcgccg gacgagcagg agcaatttga
     4261 a
//
LOCUS       DADLSB010000249         4232 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.249, whole genome shotgun sequence.
ACCESSION   DADLSB010000249 DADLSB010000000
VERSION     DADLSB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.249"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            64..1122
                     /gene="yeeE"
                     /locus_tag="JN331_004016"
     CDS             64..1122
                     /gene="yeeE"
                     /locus_tag="JN331_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY7261979.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            1136..1363
                     /locus_tag="JN331_004017"
     CDS             1136..1363
                     /locus_tag="JN331_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY7261980.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1406..2830)
                     /gene="sbcB"
                     /locus_tag="JN331_004018"
     CDS             complement(1406..2830)
                     /gene="sbcB"
                     /locus_tag="JN331_004018"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY7261981.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            3012..4190
                     /locus_tag="JN331_004019"
     CDS             3012..4190
                     /locus_tag="JN331_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7261982.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
BASE COUNT         1037 a          989 c         1090 g         1116 t
ORIGIN      
        1 ttggtctgca ttccaccagt catgaaaaat ttccccatta acattcatct ggttaattaa
       61 tttatgtttt caatgatatt aagcgggcta atttgtggtg ctctgctggg atttgtcatg
      121 cagcgtgggc gtttttgcct gactggtggt tttcgtgata tgtatatcgc gaaaaataat
      181 cgcatgtttt acgccttgct gattgcaatt tcggtacaaa gcgtgggggt ttttgcgtta
      241 attcaggcgg gcctactgac ttacgaagcc ggggcgttcc cgtggcttgg tactgttata
      301 ggtgggtatc ttttcgggct gggaattgtt ctggcgggtg gatgtgccac cgggacctgg
      361 tatcgcgcgg gtgagggatt gatcggcagt tggatcgcgc ttttcactta tatggtgatg
      421 agtgcggtga tgcgttctcc acacgccagt ggtttaaatc aaaccttgca gcactacact
      481 actgaacata actctattgc tgagactttt aatttgtctg tgtggccgtt ggttaccgtt
      541 ttgctggtga taacgctctg ggtggtgatg aaagagttga agaagccaaa actcaaagtc
      601 gcgaccttac cgccgcgccg aaccgggatc gctcatattc tgtttgaaaa acgctggcat
      661 ccctttgtca ctgctgtact catcggtttg attgcgcttt tagcctggcc cctgagtgaa
      721 gcaaccggac gcatgtttgg gttaggagtc acttccccaa cggccaatat tctgcaattt
      781 ctggtcgcgg gtgacgtgaa atacattaac tggggcgttt tcctggtgtt agggatcttc
      841 gtgggggcat ttattgcagc caaagcgagc cgtgagttcc gcgttcgcgc agctgatgca
      901 caaacaacat tacgtagcgg gttaggtggt gtactgatgg gcttcggtgc cagtattgca
      961 ggtggttgct ctatcggtaa tggactggtt atgactgcaa tgatgacctg gcagggctgg
     1021 attggccttg tatttatgat tctcggagtc tggactgcgt cctggcttgt gtatgttcga
     1081 ccgcagcgta aggcgcgact ggcaactgct gcggcaaatt aagtaaggtg taaagatggc
     1141 gattaaaaag ctggatgtgg tgacgcaggt ctgtccgttt ccactcatag aagcaaaagc
     1201 agcactggca gaaatggcca gcggcgatga gttggtaatt gagtttgatt gcacccaggc
     1261 aactgaagca atcccacagt gggcggcaga agaaggacat gccatcaccg attatcagca
     1321 gattggcgat gcagcgtgga gcatcaccgt tcaaaaagcc tgactcaaca ttgtcctccg
     1381 ccgtaccagc ggcggcggct tcaaattaga cgatctcttc cgcgtactgc caaagtgctt
     1441 ttaacagcgc cactttctct ttgtcatcgg catattgttg taccagcatc tgcaattcat
     1501 cagcataacc ctgcaaaaac tctggcgtga agacctggcg gcggtgctcc agccagcgtt
     1561 gctgctcggc ataatccagc gtccccggga agttgcgtgc ccgataattg aacaacaact
     1621 tttcaattcg tttatcaaca aaagtgatat ccagtgccgg taaattacgc ggctcggttt
     1681 ccagcacaat tttcattgct gcacgatcgg cgtcactgaa aaagccatta taaagctgtg
     1741 catccacatt atctgaaggt gtaaacggtt cggcttccgc gaatatcgct accacttttt
     1801 cacgcacttg cggattttca cgcagaattt ttaggttatc gaggcaatgc tgacgattaa
     1861 ttcccagtcg gtcggcatct tccgggcgta gcgtattcgc ctgggccagt accggacatt
     1921 tattgatatg caccagctta accggaacgg cggcgttatc gccaagatcg gcttttgcgg
     1981 tatataaacg ctcgcgcaat gtgtcgatat ccagctccag taatggcgaa atgtctcctg
     2041 ccaaatccgc cataattacg gcattgcgat tttcaggatg ccacgccagc ggtgccaccc
     2101 agctggtatt gccacgccat gccccaaaca ttccggaaac gtgagccagg ggtttcatct
     2161 gcggaacatc aatcaacgcc atcagtttgt gtttattacg atgggtaaag agataatcaa
     2221 acaggcgtgg ctgacgcgtt tttaccagtt tcgccatcgc aatagtggcg tacacatcag
     2281 ccatcgcatc gtgggcgttg ctatgttcaa taccattcgc tttggttaaa tgctcaaggc
     2341 gaaagctcgg tagaccgtca tcattttcag gccagtttat tccttccggg cgcagagcat
     2401 aacaggcacg cataacatcc agtaaatccc agcgcgagtt atcatgctgc cagctccagg
     2461 cgtaaggatc gtagaaatta cgataaaaaa cgttgcgtgt gacttcgtcg tcgaaacgca
     2521 cattgttgta gcccagaata caggtcttcg gtacggtaaa aagcgagtga atacgggcgg
     2581 caaacgcggc ttcgttttct cctttcgccc gtgcctcctg cggggtaata ccggtaatta
     2641 atactgctcc aggccggggt aaatagtcat ccgcgggctt gcagtaaaag acttcgggtt
     2701 cgccgatgac attgaattcg tcatcggtgc gaatggctgc gaactgtgca gggcgatcta
     2761 acgcggggtg cgtgccaaag gtttcgtaat cgtgaaagag gaaggtaggt tgtttatctg
     2821 tgtctgtcat tgtctagcat tgtccactgt agttaattaa ctgtctgtta taactcgaaa
     2881 aacaaccctt ttttcacctg agtatgtcta cgaacgtcaa atatagttgt ttactgtgcg
     2941 ctgttgtcca tgtgatattg tgtaccagat gtgtaccaaa ctattatttc tgagcgtacc
     3001 aaatattatt tatggctata agcgacacaa aactgcgtac tatttatggt aaaccatatt
     3061 cgggcccaca agaagtggct gatgccgatg gcctcagcgt acgaatttca ccgaaggggg
     3121 tcatccagtt ccagtaccgc tatcgctggc atggcaagcc taatcgactt gggcttggtc
     3181 gatacccatc cctgtctttg aaggatgcca gacagatcac tgctgacttg cgaaagctct
     3241 atttctcagg aacggatcca cgcacctatt ttgaagagaa ggtggagaac tccatgacgg
     3301 tcgcccagtg tctcgactac tggttcgaca actacgtctc tacaactctc agagaaaaga
     3361 cccaggcact ttatcgatca acggttatga agcgcatgca tgacgccttc cctaatcgtc
     3421 cggcatcttc tatcacggtt aagcaatggg ttgacctgct taccgaagaa gaaaaagata
     3481 atccacgccg agcaaggcag gtgctaagtc aactaagatc agcaattagt tggtgcatgc
     3541 ggcgtcagtt gatagatagt tgcgcaatta tgagcatcca gccaagggac ttcggctccc
     3601 gcgctgaggt aggggatcgg gtactgtcgt atcacgaact ggctaagatt tggcttgcta
     3661 ttgaaagaag ccgtgcgtct acgtcaaata agctccttca tcagatgctt atgctgtggg
     3721 gggcgaggct ctcagagctt aggctggcaa caaagacaga atttgacctg ctggacaacg
     3781 tatggaccgt accgaaagag catagcaaga tgggtaatgt tatccgccgt ccaatcttcg
     3841 aacaaattaa gccttttctc gaaaaggcca tgacaacgta caatgatgtt cttttccctg
     3901 gagaagacat aaacaaaccg atcagcatcg ctgcagccaa ccgattcgta aatagaataa
     3961 ggggagggat ggatctaggt tactggcgaa cacatgattt cagaagaacg cttgttacac
     4021 ggctgtccga gatgaatgtc gagcctcatg ttactgagcg aatgctcggt catgaacttg
     4081 gcgggataat gtccgtatac aataaacacg actggataga ggctcagcgc aaagcgtatg
     4141 agcttcacgc tgataaattg ttctggcaca tcaggagcat ttctgattaa cgccaccgtt
     4201 aagaatccac ccttcaacag cttcacgaag gt
//
LOCUS       DADLSB010000250         4183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.250, whole genome shotgun sequence.
ACCESSION   DADLSB010000250 DADLSB010000000
VERSION     DADLSB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.250"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN331_004020"
     CDS             complement(<1..209)
                     /locus_tag="JN331_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7261983.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="JN331_004021"
     tRNA            complement(324..399)
                     /locus_tag="JN331_004021"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="JN331_004022"
     tRNA            complement(406..480)
                     /locus_tag="JN331_004022"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="JN331_004023"
     tRNA            complement(597..681)
                     /locus_tag="JN331_004023"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="JN331_004024"
     tRNA            complement(690..765)
                     /locus_tag="JN331_004024"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="JN331_004025"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="JN331_004025"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY7261984.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JN331_004026"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JN331_004026"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY7261985.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JN331_004027"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JN331_004027"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY7261986.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1079 a         1039 c          932 g         1133 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DADLSB010000251         4107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.251, whole genome shotgun sequence.
ACCESSION   DADLSB010000251 DADLSB010000000
VERSION     DADLSB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.251"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(953..1144)
                     /locus_tag="JN331_004028"
     CDS             complement(953..1144)
                     /locus_tag="JN331_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7261987.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1208..1531
                     /locus_tag="JN331_004029"
     CDS             1208..1531
                     /locus_tag="JN331_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY7261988.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1521..2231
                     /locus_tag="JN331_004030"
                     /pseudo
     CDS             1521..2231
                     /locus_tag="JN331_004030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2209..2739
                     /locus_tag="JN331_004031"
     CDS             2209..2739
                     /locus_tag="JN331_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7261989.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQTNLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2746..2979
                     /gene="mbeD"
                     /locus_tag="JN331_004032"
     CDS             2746..2979
                     /gene="mbeD"
                     /locus_tag="JN331_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY7261990.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            complement(3524..3991)
                     /locus_tag="JN331_004033"
     CDS             complement(3524..3991)
                     /locus_tag="JN331_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261991.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          992 a          935 c         1216 g          964 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcttg gtcttttgct cacctgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cttggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagtcag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccctgacac ccgctaacac ctgctgacgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga ccgtctccgg gagctgcata tgtcagaggt
      961 tttcaccgtc atccccgaaa cgtgcgaggc agctgcggta aagctcatcg gcgtggtcgt
     1021 gaagcgattc acagatatca gcctgttcat ccgcgtccag ttcgttgagc ttctccagca
     1081 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttag
     1141 tcactgatgc ctccgtgtaa gggggatttc tgttcaggag gtaatgatac cgatgaaacg
     1201 cgagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgctg
     1261 tgagggtaaa cagctggcgg tatggatgcg gcgggtctgc ctgggtgagc cggttgcccg
     1321 ttccgggaaa ctgccgacac tggcaccgcc gttactgcgt cagctggctg ccatcgggaa
     1381 taacctgaat cagacagcga ggaaggtgaa cagcgggcag tggtcttccg gtaaccgggt
     1441 tcaggtggtg gccgcactga tggccatcgg ggacgagctg cgccggctgc gtctggccgt
     1501 cagggaacag gggacgcggg atgatagtta agtttcatgc ccggggaaaa ggtggtggca
     1561 gtggtccggt tgattacctg ctgggaaggg agcgtaaccg ggaaggggca acggtgcttc
     1621 agggtaatcc ggaagaagta cgggaactca tcgatgccac gccatttgcg aagaaataca
     1681 cgtccggtgt tctgtcattc gcggagaagg agctgccgcc gggaggacgt gaaaaagtga
     1741 tggcgagctt tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt
     1801 gggtggagca ccaggacaag ggacggcttg agctgaattt tgtcattccg aacatggagc
     1861 tacagaccgg aaaacgcctc cagccgtact acgaccgcgc agacaggcct agaattgatg
     1921 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
     1981 gcaggacgct gacactccct gataatctgc cggaaacgaa acaggcgatt gctgagggcg
     2041 ttacacgcgg gatagacgcg ctttaccatg ccggagagat aaaaggccgt caggatgtga
     2101 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
     2161 ttgcagaccc gaacggcggg aagaatatca gactgaaagg agcattctat gagcaatctt
     2221 ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
     2281 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa gcaaggctgt gacatcaaac
     2341 gagacgaaaa tcaaagacgc tatagccctg ttcacagctt cgacagagga atcactgaaa
     2401 aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg
     2461 gcagggaata cgggcatgat gttactgggg atggtcattt tcctgtttac cgtgagtggc
     2521 gggacgctct ggtatcttgg cgggatgata cagacgaacc tggaagaaat caggaagcag
     2581 aacgagagtc tggagaagct gaacgcgaag acatggggcg tgcgttatca cgaggggagc
     2641 aacggacgtt ttctggtgtt gcccgagggg atgaaagccg aaacgaactg gacgatggaa
     2701 aacgggaaac agaatgccgt aaggctggta caggagtaac agagcatgac ggagctggaa
     2761 agacatttgc tgaacgcatt agagcaactg caacaggact atatgcagcg gctgaacgaa
     2821 tgggagagcg ccttcgtgga attgcagaaa atgttttcgc ttacgcaacg ggacaacgcg
     2881 atgctgaacg agcgggtcat gcagttgagt cagcaagtgc agcacttgag cgagcggaca
     2941 gaacgcttga gccagttata cagcgagaac aggagataag ggacgaacgg ctgatacagg
     3001 aatgcgaaca tgcgttatcc ctggagcgtg agcgccagct ggaaatacag gaacggacac
     3061 tggatggccc ttcgctggga tggtaaccag agacaggcta aaatgttggc cgcgcgtttc
     3121 cggtgcattt tttgggataa tggttagttg ggggcaccgg gaacgcgctt cagtcagacc
     3181 ttgctgaaaa cacaggaacg cgttaaaacg catacgggcg cgattctggt gtgtcttttg
     3241 ttgctgtgtt ttgcctctat tgccagatcg cgctcattcc cctgaacttc tgttttcccg
     3301 ctcaggtccc cgtcttcttc gcttccgaaa gcatgaaaaa aatccgtttc agctgtctct
     3361 ggctggcaat tcctgcgtga ttcacatggc tgcatagcta tgcatgtggt taaaatttac
     3421 cgggtgcgat cgcggcagtt tttcgggggg gttgttgcca tttttacctg tctgctgccg
     3481 tgatcgcacc gaacgcgttt cagcggtgcg tacaattaag atattacgtt aaattgtgag
     3541 ggaatggtcc ccatatactg actttatcat agggataggg ttggtagtgg gtcgattggg
     3601 tttcggcatt gtttccgtta atgccaattg catttttgta tttgatgcaa cagaaggcgt
     3661 gagattctcc tgggcctgga aaggataagt gtttagaatt gatggcaaaa tatcttccgt
     3721 ccgatttttg aagtagttgt tgtttggctg tctcccaaag ggtgctgtta caaacttctt
     3781 gaatttgtag tttttgcagg gctcttatta ctgcgttgtc atgttccaat tctttagggg
     3841 agtgtattat tctctctttt aataagctgt ttaccacagg gtttagttgt tgtctaaagc
     3901 acgcagataa tgccattgct acacaatttt gttcccgagg aagcatgttt ggcggggtat
     3961 gccgtgtttg atgtgatatg tctattctca tagaaggtct ctggcttata gcatcaacaa
     4021 atacggctat aaaacaaaat tttcatagag tgattcaaga tcttcttgag atcctttttt
     4081 gtggcgcgta atctgctgcc tgcaaac
//
LOCUS       DADLSB010000252         4094 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.252, whole genome shotgun sequence.
ACCESSION   DADLSB010000252 DADLSB010000000
VERSION     DADLSB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.252"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..293
                     /locus_tag="JN331_004034"
     CDS             <1..293
                     /locus_tag="JN331_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261992.1"
                     /translation="MGLANKGWIKGEPQGGGWMIKPLGRWSLIMEIDEGFAVGMSPAE
                     LSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            303..545
                     /locus_tag="JN331_004035"
     CDS             303..545
                     /locus_tag="JN331_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAY7261993.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKTLSGVMKIFHRSGSPPLRRPST"
     gene            581..3948
                     /locus_tag="JN331_004036"
                     /pseudo
     CDS             581..3948
                     /locus_tag="JN331_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1079 a         1039 c         1017 g          959 t
ORIGIN      
        1 tcatggggct ggcaaataaa ggctggataa agggcgaacc acagggtgga ggctggatga
       61 tcaaacctct cggtcgctgg tcgttaatca tggaaatcga cgaaggtttt gcggtgggta
      121 tgtcaccagc cgaacttagc gctgagcagc tcttaagcaa gctgtggcta tgggaaggca
      181 aagcggaaag ctatggctgg gggagtaatt caacacagga agcgcagttc tccgtactcg
      241 atgccatcac cgccagcgag ctaattaacg atattgaagc cctgtttgaa taaggaaaga
      301 gcatggacaa ggaattaccg tggctggcgg ataacgccca actggaactg aaatataaaa
      361 aaggcaaaac accactcagt catcgcaatt ggccgggcga accagtgcct gttatcactg
      421 aaagcatcat tcagacactg ggtgatgaat tgctacaaaa agctgagaag aaaaaaacat
      481 tgtctggcgt tatgaaaatt tttcaccgga gtggcagtcc gccattacgc aggccatcaa
      541 cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcac tagcctttat
      601 cgcgcaaaat gacaaccaac agttgctcga cgaaatcgtc caacaagagg ggctagaata
      661 tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga gtgatcctct
      721 ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct cagaaaccta
      781 taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagaggaaa gctgctggca
      841 gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt
      901 tattgcgctg atcctccccg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg
      961 cccgcggact cattttcatt ctaaggagtg gttaaaagtt gatgctactg accccaaagc
     1021 ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcgag aagccagcta
     1081 catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg
     1141 actggcagtc gttccgcgcc tcgcgatata tgcccataaa gaagattgcg gcagtctgct
     1201 ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg ataaaaacaa
     1261 acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga cactcgccgc
     1321 actggcagaa ctgctggcgt taaaagaacc accagcccgc ccaggttatc caatcatcga
     1381 agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta cgctgttaca
     1441 aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt taagtactca
     1501 agcccgggca gtgctgaata gttatttatc ggcaccgccc aaaccagtta ttgatagcac
     1561 cgataacagc caaatgcctg aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa
     1621 gacttccata cgtcactttt cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc
     1681 gccgaataaa cactggacgc cggaacagca aactgcaatt gaccatttta gcgccatgtc
     1741 ttttgacgaa cgtcttgcta cgagaggagc ggatatcttc ttgcgggaat taggttttga
     1801 cgttcatagc ctggaattta ataagcgtct tcttaatgga cagttggatg cctttgatgc
     1861 tcagcaactg caagaataca catcaatagg actattgcgt acttatcacg cgcatcaggc
     1921 caaatctaaa cagtaccggg aagacgccac caatgcatta ctggcacaag atagcattgc
     1981 gttgatggga gggtggcgaa cttttcagca cggattttac tatggtaatg aacagcgttg
     2041 ggggatttgg aatctgtacc ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg
     2101 gcaacaaatt gtcgaggctg attttcatta caccggggtg gaatatttgt taagtgtgct
     2161 gggtaccgat gctttgcctg gattaaacac cgcctttgcc cgacatccaa aagagatatt
     2221 tccgctgctg attcatttcg gcgcaacaga actggccctg cccattgccc gcgtctggca
     2281 cctttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc cggaacatac
     2341 ggctactgca cttatccctc ttgtcttcac caaatctagc gataaaagcg aagctgcatt
     2401 acttgccctg cgtttacttt acgaacatgg gcatggcgaa ttgctacaaa ctgtagccaa
     2461 tcgttggcag cgtacagatc tatggcctgc cctggaacaa ttacttaaac agagtccaat
     2521 agagatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc
     2581 caggccgcgc cttatcacta ataatcaacc tgttaccgat gacgctctgg aaattatcgg
     2641 cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac
     2701 gttctgccag ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca
     2761 agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga
     2821 aagcacggca cgggatctaa cgacacatat cctcgcctgc ccacaagaag gcaaatctgc
     2881 ccgtgcggtc agtggcctga acatccttac cctgatgaat aatgatatgg cgctgatgca
     2941 gctgcatcat atatcgcaac gggcgaaatc ccgcccctta cgtgataacg cggcggaatt
     3001 tcttcaggta gtcgcagaaa atcgcgggct aagccaggaa gagttagcgg acagattagt
     3061 cccaacactg ggccttaatg atccgcaggc gttgagtttt gattttggtc cccggcagtt
     3121 taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca
     3181 gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc
     3241 ccgactaaaa gggttaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct
     3301 tgaagctgcc ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt
     3361 taatcatccc tttacccgtc tggttaccca gcgattaata tggggggtta tccggcaaat
     3421 gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg
     3481 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
     3541 atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
     3601 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
     3661 agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg aatgcgatac
     3721 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
     3781 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
     3841 ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg
     3901 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa caaagcgggc
     3961 agtgatgcga ctgcccgtta tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
     4021 aaagagctgg ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc
     4081 agacttgaga tcca
//
LOCUS       DADLSB010000253         3999 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.253, whole genome shotgun sequence.
ACCESSION   DADLSB010000253 DADLSB010000000
VERSION     DADLSB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.253"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            293..889
                     /gene="wrbA"
                     /locus_tag="JN331_004037"
     CDS             293..889
                     /gene="wrbA"
                     /locus_tag="JN331_004037"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7261994.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            910..1137
                     /locus_tag="JN331_004038"
     CDS             910..1137
                     /locus_tag="JN331_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261995.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(1175..2416)
                     /gene="agp"
                     /locus_tag="JN331_004039"
                     /pseudo
     CDS             complement(1175..2416)
                     /gene="agp"
                     /locus_tag="JN331_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
     gene            complement(2709..3965)
                     /locus_tag="JN331_004040"
     CDS             complement(2709..3965)
                     /locus_tag="JN331_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY7261996.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
BASE COUNT         1111 a          924 c          841 g         1123 t
ORIGIN      
        1 gggactaagc ttagtgaact gacgcgggca gacccgcgtt tggtaatatc ctgcaacagc
       61 ttcttagcga gaatagatat catctccaat aattatccat aagccgctct tatgattaaa
      121 aaaacgcgat gagataaaaa tgtgtcattt tgcgacaaaa ttacgtgctt gtgaaagttg
      181 ttataaatca aataagtggt tgtgaaattt gcactctgaa aaggacgtct tatctttaaa
      241 taagtggtag cgaatcgcta cggaatagag ataacacgag gagtggttag aaatggctaa
      301 agttctggtg ctttattatt ccatgtacgg acatattgaa acgatggcac gcgcagtcgc
      361 tgagggtgca agcaaagtgg atggcgcaga agttgtcgtt aagcgtgtac cggaaaccat
      421 gccgccgcaa ttatttgaaa aagcaggcgg taaaacgcaa actgcaccgg ttgcaacccc
      481 gcaagaactg gccgattacg acgccattat ttttggtaca cctacccgct ttggcaacat
      541 gtccggtcaa atgcgtacct tcctcgacca gacgggcggc ctgtgggctt ccggcgcact
      601 atacggaaaa ctggcgagcg tctttagttc caccggtact ggcggcggtc aggaacaaac
      661 catcacatcc acctggacga cccttgcgca tcacggcatg gtgattgtcc ccattggcta
      721 cgcagcgcag gaattatttg acgtttcaca ggttcgcggc ggtacgccgt acggcgcaac
      781 caccatcgca ggcggtgacg gctcacgcca gccaagccag gaagaactgt ctattgctcg
      841 ttatcaaggg gaatatgtcg caggtctggc agttaaactt aacggctaat cttcaacagg
      901 aggatacgca tgccaactca agaagcgaaa gctcatcacg tcggtgaatg ggcatctctg
      961 cgcaatacgt cgccggaaat agccgaggcc attttcgaag tcgccgggta cgacgaaaaa
     1021 atggcagaaa agatttggga agaaggtagt gatgaagtgt tagtcaaagc ctttgctaag
     1081 accgataaag attcgctttt ttggggcgaa cagaccatcg aacgtaaaaa cgtttaacca
     1141 gcgactcccc cgcttctcgc gggggagttt tctgttattt caccgcctca ttcaacacgc
     1201 tatcaaactt atccatcggg cagaaaccgt tggcatctat cgggcaaccg cttaattcca
     1261 gcgtcacgcg ctgcgcaggt gcctgcaggg ttaacgcatc ggcattacgt aactgttccg
     1321 cactctggta cacatattca attttcatca aatcgcgatt ggctttgctg tcatgccaac
     1381 gctggaaaac gattttgccg ccaatcggcg tgcgttcgtt ctggtcatgc aactgatacg
     1441 gtttgaaatc cagcgccgtt aacagagagg ctatgttgga gtcgtgccca actaacaccg
     1501 tgattttcgg agcactggcg cgatcggtga ccagggcttt gtcgatataa ctgaccagcg
     1561 gtttcgcaac attgcgcgcc acttccggtg aggtaaacag gctgtcctag taaccgtttt
     1621 tcagcttcga caataccttc cactgctggt cagatttgat ttctccctag gccacctgat
     1681 ccatcggaaa accttcgtag tattgcaaag taaacgcatc taccagcgag ttgccgactt
     1741 tcagcggccc ggaaacacct ggttcttgtt gatacttggc gctaaaggta tttttgccat
     1801 ccaccagcga acactgttgt ttctctttac aggcagggga atctttatag ttaacgattt
     1861 tttccagtag ctggtagctg tcggtaagct ggagtttgct gagctctttc tccattgccg
     1921 ccaccgcctg ttcactgaat gcggcggaat catcggtgat catcgggtta aaggttgggt
     1981 ccatagtgcc cattttttcc tggtgatgca caggaatatc acacccaggg aatgcaccag
     2041 taataaagaa ctgggcggta gcgacggtac gttgcagact attggcatag gcataaacgg
     2101 tgtccggcgg cgggcattcc cccgatttca ctatcccctg ctctgccagc cattcacgca
     2161 tgtaatggcc catatacact tcgagcacgc cacctttggt ggtgagttgc ccaccgggga
     2221 cgtcccattc gggccattta ttcggcgtcg actgctccag cacactgcca ttgttcgcca
     2281 gcggcgcacg taagttatgg cggctcatca tgagcacttg ctgtagctga tagccttccg
     2341 gtacggtttg tgcctgagca tttgaagcga gtaaaactat ccctgccaca gttgcggcga
     2401 ttagcgtttt gttcattcct ggcacctctt ttgttatcaa tcattaaaga gtgtgacaga
     2461 aatatgactt ttttccggga acaggttcgc aaaatggctt gttgaacgcc tgtttttcta
     2521 tatacatctt caaaaacagg caaggtttga cgcaaactat gcctgattag cccctgcctc
     2581 gcgtacgccg ctgataattg caactgtggc taaacagaat atctgcggcg ttaccaatac
     2641 agagacaata tttaccaata aaaaagtgtg agactaataa caatggtttt tagcatatgc
     2701 ctttgccatt aagttgcata acgatttaaa tagtcaagtt gcgcattcat ttccttattt
     2761 tttaacttat aagcctgaga aaaaagttca ggagggaggt aaacatcata atgattgcca
     2821 gtgtttatta aataaccata ctcaagatct tcatctgttg aaaaggattc atttcccaag
     2881 gagattgatg ccacattact attaacaata aataatgagc aaatgctatc tgaaagaaca
     2941 gcaagaaaat tcttcaccgc tttaatccta tccgacgtgt cggtatctaa agcaagcaaa
     3001 ttcttgttat cattaaatat aaactcgggt aaatgcagta tgcatttttt gtcattcaag
     3061 caactatcaa tgaattgttt tttaaaaaac agaaagctcc atgacataaa actacccaca
     3121 ccctgaattt tgttgtcaga ttgcagatca acgccaaata atgtctttac atcttccctg
     3181 agattataga atgccgtatc aacaagagcc tcgatcgttt gtttatcaac aattttaata
     3241 aactcttcgt atacttgctt ttgcgtctta tggctgccca gcgcccatga atactcatgt
     3301 tcttttaaaa ccagtatggc tcttaacaga caatatccat cgccatctac tggagcgtat
     3361 tttaatccat atgttttatt aatttcactc tgaataaaag atatgccaga caaatcaata
     3421 ccagtatcta aatcagatat tcccggcata tttatctgtt ctggctggat actcatattt
     3481 tcatcatcta aacaatactc agaaaaaaca tcatcacacg cattaatgtt ttcaatatat
     3541 tccataaagc cataccaata ataaccttta aaatctgtga tagactctga acctctgccc
     3601 ggcaaggaca ctctaacaat atcaacatct tgaacatatt gtatagtata tgtaatacct
     3661 cctgaaaccc ctgaataaat ttgtgtatca ttagtacgca atgttctcat aaataatgat
     3721 agatcattat cattctgcat ttcctcaaat tctttttcta gtgattcaca ttccagacat
     3781 ggagtatcct cttcatttac tttacgtctg agttcattgt acaaccgcat aaaacacacc
     3841 agtgtgtttt caaaaagcgt gattgaatat gtttgatttt tttcgtactc attttttata
     3901 tcgttccagg cctgtttcaa ataattattt gcagtgcaac taattccatg tatattacta
     3961 cccatatata gcgtctataa aatttaataa ataatgacg
//
LOCUS       DADLSB010000254         3918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.255, whole genome shotgun sequence.
ACCESSION   DADLSB010000254 DADLSB010000000
VERSION     DADLSB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.255"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            156..908
                     /locus_tag="JN331_004041"
     CDS             156..908
                     /locus_tag="JN331_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY7261997.1"
                     /translation="MPVSLSAQLGSKEQADVRLAGSVMSALRVSMPGIVQSFDPDTVT
                     VVVQPAIKGYEPDSNGINQSTTLPLLVDVPVVFPRGGGCTLTFPVKAGDECLVVFADR
                     CIDFWWQSGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTRKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGAVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSNTDGVN"
     gene            908..1261
                     /locus_tag="JN331_004042"
     CDS             908..1261
                     /locus_tag="JN331_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261998.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGKQKPETYNLAIRKRILETRGVKSILSFNTTVDTTTRRVRFST
                     EIDTLYGITTVTSEA"
     gene            1261..2460
                     /locus_tag="JN331_004043"
     CDS             1261..2460
                     /locus_tag="JN331_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7261999.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNMAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDGNNVIWRLPASVVVGVDGTVMATARCSVSGAVAALA
                     GTITEINTPTRGWVSVINPAAATVGSPAETDAELRIRQSQSVALPSITPFEALDGAVS
                     NVAGVTRHKLYENDTGSEDGNGLPPHSVAVIVDGGDVTDIAQAIRGNKGQGTATHGTT
                     SVTVPDKYGNPHVIKFSRSSDVPVYARIKLKVFTGYTSQIGQQIQQVISDYINSLTIG
                     DSVLLSRIYSPANLGVVSGWNARYYDIQELTIGKSPGALSSSNIDIRYNESASCTPEN
                     IVITVES"
     gene            2457..3137
                     /locus_tag="JN331_004044"
     CDS             2457..3137
                     /locus_tag="JN331_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY7262000.1"
                     /translation="MSKYTELITNYHATKPKFLAHVDLMTRPLIDVAAATRGLITAFD
                     IDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYL
                     SDETYRVILKAKIAINNWDGRNDSLPAILDAATAGSGLRMQIVDNQDMTISVWVFPDT
                     DISDVSCELIAAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFD
                     VGAWGILL"
     gene            3464..3918
                     /locus_tag="JN331_004045"
                     /pseudo
     CDS             3464..3918
                     /locus_tag="JN331_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          991 a          827 c         1063 g         1037 t
ORIGIN      
        1 taaaaaaaga agaatgtaga ttggtgctat ctgaaaataa gaaatgagtt cgctaaatta
       61 acttttcacg gtaattgctt ctatcaccat ggaaagaaaa ctagctacat aaaacccgcc
      121 ttcgagtggg ttttttgctt tctggaggca tatgaatgcc agtttcttta agcgctcaac
      181 ttggtagcaa agaacaggcc gatgtaagac ttgctggctc tgtcatgtcg gcgctgcgtg
      241 tttctatgcc cggcatcgtc cagtcatttg atccggacac ggtaacggta gttgttcaac
      301 cagcgattaa aggctatgag ccagactcaa atggaatcaa tcagtcgacg acattaccct
      361 tgctggtgga tgtgccagtg gtattcccgc gcggcggagg ctgtacgttg acttttccgg
      421 taaaagccgg ggatgagtgt cttgtcgttt ttgccgatcg ttgtattgat ttctggtggc
      481 agagtggcgg gatacaggag ccggttgatg acagaatgca tgatttatcg gatgcgtttt
      541 gtattgtagg tccccagtcg caggcgagga agattagcgg tattaatacc agtgccacac
      601 agttgcgtag tgacgacggc agtacctatt ttgagcttaa tcctgatacc aggaaaatta
      661 aaattgtcgc tccgggtggt cttgatgtgg ttgcccctct ggctgatttt tctgagaaag
      721 taaccattca tggcctgtta acctggatgg gtggcatggt ggggtctgtt gtttctggtg
      781 tggcttcaaa aatcactggt gctgttgagt ttttgggtag cgtgaaggct aacggcaagc
      841 caatcgatga tacgcacact catggtggtg ttcagcgcgg tggaagcaat accgacggag
      901 taaactgatg cgatacagac gtgaagacgc cgatggcgat tacacctttg gcagcggtga
      961 tgacacctgg ctgattaact caccggaggc cgtggcgcag gcggtaaaaa cgcgattcga
     1021 attgtggtat gggcaatggt ttctcgacac caccgagggg actccgtgga tccagtccgt
     1081 actcggtaag cagaagccgg aaacctacaa cctggcgatc cgtaagcgca tcctcgaaac
     1141 gcggggcgtt aaatcaatcc tctctttcaa tactacggtg gataccacga cccgacgtgt
     1201 caggttttcc actgaaatcg acactctcta tggaataacg actgttacat cggaggcgta
     1261 atggctctga accttgattc tctcggttta tctgcaaagg taaccgcgga ggggatcagt
     1321 gcgcctgatt atcagacgat actcagcacc ctgattagct attttcagca gatttatggt
     1381 agtgatgcct acctcgaacc ggacagtaaa gacggtcaga tggtggctct gatggcgctg
     1441 gcgattcatg atgccaataa tatggctata actgtctaca actgtttttc accggcaacc
     1501 ggctatgggg ctgcactgac cagtaacgtg aaaataaatg gtatttcacg taaaggcgcg
     1561 acgaactcta cggttgattt gcttcttaca ggaacagccg gaacaaccat cattaatggc
     1621 agcgtgaaag atggtaataa tgtgatatgg cgtttgcctg cgtcagtggt ggtcggtgtg
     1681 gatggtacag tgatggcgac cgcaagatgt tccgtcagtg gtgcagtggc ggcgctggct
     1741 ggaactatca ctgaaattaa tacgccaacc cgtggctggg tttcggtaat taatcctgct
     1801 gcggctactg ttggctctcc ggcagaaact gatgctgagt tacgtatccg ccagtcgcaa
     1861 agtgttgcgt tgccatcaat aaccccattt gaagcactgg atggtgctgt ttctaatgtt
     1921 gccggtgtaa cccgccacaa actctatgaa aacgatactg gttcggagga cggtaacggg
     1981 ttaccgccac actctgttgc tgtaattgtg gatggcggtg atgtgacgga tattgctcag
     2041 gctatcagag ggaataaagg ccaggggaca gccactcacg gtacaacatc cgttacggtt
     2101 ccggataaat acggcaatcc ccatgtaatc aaattctcgc gttccagtga tgtgcctgtt
     2161 tatgcccgga ttaaattaaa agtttttacg ggttatacct cacagatagg gcagcagatc
     2221 cagcaggtta tttccgacta tatcaatagt ctgacgattg gtgattcggt ccttttaagt
     2281 cgcatttact caccggcgaa tcttggcgtg gtgagtggct ggaatgcacg ctattacgat
     2341 attcaggagc tgacgattgg gaaatccccg ggggctttgt cgtcatcaaa cattgatatc
     2401 agatacaacg aatctgcgtc ctgtaccccg gaaaatatcg ttataacggt ggagtcatga
     2461 gcaaatacac cgaactaatc acgaactacc acgccaccaa acctaaattt cttgcgcatg
     2521 ttgatctgat gacccggcca cttattgatg ttgcggctgc caccagaggg ctgattactg
     2581 catttgatat tgactctgcg gttggtgtgc aacttgacat tctgggattg tggatcggac
     2641 gtagccgtgt tgtcagccag cctatctcag gtgtctattt cagctgggat accgacgggc
     2701 ttggatatga tcagggggta tggcagggac catacgatcc tgattccgga tacatgtacc
     2761 tcagcgatga aacttatcgt gtcattctta aagcgaagat tgcgattaat aactgggatg
     2821 gacggaatga ttcgcttccg gcaattcttg acgctgcaac agcaggatcc gggctgcgaa
     2881 tgcagatagt cgataaccag gacatgacga tatcggtctg ggtctttcct gatactgata
     2941 tttcagatgt atcgtgtgag ttaatagcgg caattaaaca ggggtatctc acagtaaaag
     3001 ccgccggggt atgggcgggt ggcattgaaa caccttcggt ggaaacccca tcggaaggct
     3061 caaaattttt tggttttgat atggataacg aattcatcag tggttttgat gtaggagcat
     3121 ggggaatatt actctgatgg cgaaaaatga ctttaaagcg tttgcaacgg gtaaaaatgc
     3181 caatgttatg tcgcaggagg aatgggaagc gttgcctgcg cttttatccg gatttacagc
     3241 agggaaagca tccagtgcgc aagtcaataa ggttattcgg caggccagct ttattgctgc
     3301 agctctggcc cagtttgtaa gtgacaaaac gcaacgggat gtgcttgata atggtgatct
     3361 gcccggtttt gttgaattgc tgggatcggg gtttgctgtt gaatacctga gccgcaagaa
     3421 tccgtttggc gatatcaaat cggatggcac ggtgaaaacg gctctcgaaa accttggttt
     3481 acaggaaacg gtaaacaagg ctggtaacgc tgtccaaaag acaggcgata ccttgtccgg
     3541 tggacttact tttgaaaacg actcaatcct cgcctggatt cggaatactg actgggcaaa
     3601 gattggtttt aaaaatgatg ccgacagcga tactgattca tacatgtggt ttgaaacagg
     3661 tgacaacggc aatgagtatt tcaaatggag acaccgcctc gctggcggcc agctcaaaga
     3721 gctgatgaat cttaaatggg attcactaaa tattctggtt aatgccgtca ttaatggttg
     3781 ccttggcatt ggtacgacga atgcgttagg tggaaattca attgctttcg gggataacga
     3841 taccggcctg aaacagaacg gcgatggtct gctggatgtt tatgcgaatg gacagcatgt
     3901 atttcgtttt cagaatgg
//
LOCUS       DADLSB010000255         3918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.256, whole genome shotgun sequence.
ACCESSION   DADLSB010000255 DADLSB010000000
VERSION     DADLSB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.256"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..374
                     /locus_tag="JN331_004046"
     CDS             <1..374
                     /locus_tag="JN331_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262001.1"
                     /translation="SPLEQSGSPKTKFPEIKTVKTAVVKLISDIEGNLDQRLVYREAV
                     EGLCQEAANRITQCMHSRSTGQTLRDLRELLSVTLDTIIVVEFEGEQTRLNYGGRSAE
                     DVIAMLKALHLEGRKAWLEVE"
     gene            complement(406..933)
                     /locus_tag="JN331_004047"
     CDS             complement(406..933)
                     /locus_tag="JN331_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY7262002.1"
                     /translation="MRKTTLLVLAAALCVPAFATAAPVLTAGFSPSDGRPALEIVLGA
                     INNARQSIDVAAYSFTSKPVATALAGANRRGVAVRVVADEKANSDRYTAVTYLINQGV
                     PVHLNGRYAIMHNKFMVIDGKNVQTGSFNYTASAVSRNAENVLLIEDAPQLAEAYQRE
                     FNRLWDEGIPLNALY"
     gene            complement(926..1672)
                     /locus_tag="JN331_004048"
     CDS             complement(926..1672)
                     /locus_tag="JN331_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HAY7262003.1"
                     /translation="MLISSYQERYARSTEKIRVLLNFLKEETYSDFQIIKLLFDFKSD
                     RPLYRLLEKVEKMGLIQKHVHESRASRISLWGITTDGLAVILTADDNIMPARFEPYRL
                     TGWSLDHHLDNQLVRLTLEKNGATGWINGDRSTFLSQYSVKHRPDGLISLPDGRLIAV
                     ETERRIKTKARYQSIMASHLLAISDKKWRYAFYVAPDEAKKRALKIIFDSIASVIVKN
                     QHVQLEAKHRDVFRFYTLDELKNLELDNYA"
     gene            complement(1687..3594)
                     /locus_tag="JN331_004049"
     CDS             complement(1687..3594)
                     /locus_tag="JN331_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAY7262004.1"
                     /translation="MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFS
                     AKHGGQEKPTFLQRILILLSMICFVFGSIAPVAVWFLQRIYGREDIASPPLLTWTMAL
                     LFTVAGFILHVVLRRLISPEFDKVKCGMVKKTSMERDRRTDVRRVKEILPVTTEYDPM
                     DYIDLNKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILLYQSILADE
                     GVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAG
                     FSLAEKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGK
                     TEELAMLNSINAPGGFALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRD
                     RVAGNIRPIAIFKDEVKYHLSKPALEGLGAARDKGVHILMAHQSIADLRDCPADLNGD
                     AVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDESRKVTTTAVLTEKVDNERM
                     LRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLPPVAVAT
                     VAEVKPVLDLDFDKTAPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPA
                     PNDEIHSLLDL"
BASE COUNT          865 a         1067 c         1089 g          897 t
ORIGIN      
        1 tatccccgct ggagcagtca ggctccccaa aaacgaaatt tcctgaaatc aaaaccgtca
       61 aaacggcggt ggttaaactg atcagcgaca tcgagggcaa cctcgatcag cgtctggtat
      121 atcgcgaggc cgtagaggga ttgtgccagg aggcggccaa ccggataacc cagtgcatgc
      181 attcacgcag tacagggcag acgctacgtg atctccgcga gctgctgtcg gtgacgctgg
      241 acaccattat cgtggtggag tttgaagggg agcagacccg tctcaactat ggcggacgtt
      301 cagcagaaga cgtgatcgcc atgctgaaag cgctgcatct tgaaggacgc aaagcctggc
      361 tggaagtgga gtaaaaaaaa agccccgtcc gcagacgggg ctgattcagt acagggcatt
      421 cagcgggatg ccctcatccc acaggcggtt aaattctcgc tggtacgcct ccgccagctg
      481 cggcgcatcc tcgatcagca gcacgttttc cgcgttgcgc gaaacggcgc ttgccgtgta
      541 gttaaacgac ccggtctgga cgtttttgcc gtcgatcacc ataaacttgt tgtgcatgat
      601 ggcgtaacgg ccgttaaggt gcaccggcac gccctggttt atcagatacg tcacggcggt
      661 gtagcggtcg ctgttcgcct tttcgtcggc caccacgcgc acggccacgc cgcgccggtt
      721 agcaccggcg agcgccgtcg cgaccggctt actggtgaag ctgtaggccg cgacgtcaat
      781 gctctgcctg gcgttgttta tggcgccgag cacgatttca agagcagggc gcccgtcaga
      841 cggcgaaaaa cccgccgtca ggacgggcgc ggcggtggcg aacgcgggca cgcagagggc
      901 ggcggccagc accagaaggg tggttttacg catagttgtc cagctccagg tttttcagtt
      961 catcgagggt gtaaaagcgg aaaacgtcgc ggtgttttgc ctccagttgc acatgctggt
     1021 ttttcacgat cacgctggcg atgctgtcga aaataatctt cagggcgcgc tttttcgcct
     1081 cgtccggggc gacgtaaaac gcgtaccgcc attttttatc gctgatagcc agcaggtggc
     1141 tcgccatgat ggactgatag cgcgcctttg ttttgatacg tcgttcggtt tcaacggcga
     1201 tcagcctgcc gtccggcagg ctgataagcc cgtcgggacg gtgtttgacg ctgtactgac
     1261 tgaggaacgt tgaacggtcg ccgttaatcc accctgtcgc gccgttcttc tccagcgtaa
     1321 ggcgcactag ctggttatca aggtgatggt cgagtgacca gccggtcagg cggtagggtt
     1381 caaagcgggc gggcattatg ttgtcgtccg ccgtgaggat aacggccagc ccgtcggtgg
     1441 taatgcccca cagggatatt ctcgacgcgc gggattcatg cacgtgcttc tgaatcagcc
     1501 ccattttctc caccttctcc aacaaccggt agagagggcg gtcagattta aaatcaaaaa
     1561 gcagctttat gatttgaaaa tcgctgtagg tttcctcctt gagaaaattc agcagtacgc
     1621 gaattttttc agtgctgcgt gcataacgtt cctgatacga cgaaataagc atagaaactc
     1681 cttattttat aaatccagca acgaatgtat ttcgtcattt ggagcgggct gtgatgccat
     1741 cagccactcc tcgtcaggtt gcaccggcgg agtgggtatt ggcgttgctg agggtttttt
     1801 ggtggttgtt gcgctgaccg gaataatgtc aggtgccgga actgccggag ccgttttatc
     1861 aaaatccaga tccagcaccg gtttaacttc tgccaccgta gcgactgcga ccggcggcag
     1921 ggtaaacagc tccagtgggg cttttttaac cttgagcggt gagattaacg acgggaacgc
     1981 gagcgcgttc tggctgaaga tatagctgac gaacttcggc aggttctgga gcatattgct
     2041 gtcgatgtaa tagctttccg cctgccgaag catgcgctcg ttatcgactt tttctgtcag
     2101 caccgccgtg gtggtgactt tccggctttc atcatcgact aaaatcgtgc ccgacatgcg
     2161 cgcaatccat tcggcggtgt ctggatcctg aagtttatat accagcttaa acttgcagtt
     2221 ttccacaacc gccccaacca cggcgtcgcc gtttaaatcc gccggacagt cgcgcaggtc
     2281 ggcaattgac tggtgcgcca ttaatatatg gacgccttta tcgcgtgccg cgcctaaccc
     2341 ttcgagcgcc ggtttggaca ggtgatattt cacctcgtcc ttaaagatgg cgatggggcg
     2401 gatattcccc gcgacgcggt cgcgcaattc cgcgagctgg aacaggcgaa ctaaaatcat
     2461 gcgctgcgcg gtaatgattt tagagttacg catcgaaccg atgatatagc agcagccgcc
     2521 cttgtcgaaa atctccttca gcgcaaaacc gcctggcgcg ttaatgctgt tgagcattgc
     2581 cagctcctcg gttttaccgt gaaaggcttt aatcgtctcg ccaatgccct gaacataatc
     2641 gccgttataa atatcccgca cggttgcgcc cggattatcc ttcacgaact gcgccgccgt
     2701 tctcgccgcc ttgcggtcgt cgatacggta aaaatccgcc gcctcgcctt tttccgccag
     2761 actgaaaccg gccacgaata attcctccag atggtcggcg gtgataccgt cgataaggtt
     2821 catctggtat tcctgtttat tcaggtcgat aagcacaaag ggttttccgg cgtccgcgca
     2881 cgctttttta tacaggtgcg gtgcccactc gtcgtttttg gggtcgagaa caaaaacgcc
     2941 ctcgtcggcg aggatgctct gataaagcag aataccggtt acgacgcctt taccggcacc
     3001 agtggtgccg ataatgtcgg cgtgctgggt ctgccattct tttaacggaa tatattgcgg
     3061 ctcaccctcg cggctcagcc cgataaaaat ccccttattc agatcgatgt aatccatcgg
     3121 atcgtattct gtggtgacgg gtaaaatttc ttttacccgg cgaacgtcgg ttctgcggtc
     3181 gcgctccatg ctggtttttt tcaccatgcc gcatttgacc ttatcaaatt caggcgagat
     3241 caaacgccgt aaaaccacgt gcagaataaa accggcgacg gtaaacagta gcgccattgt
     3301 ccaggtaagc agcggcggac tggcgatatc ttcccggcca taaatgcgct gtaaaaacca
     3361 gacggccact ggcgctatcg agccgaaaac aaaacagatc atggaaagca gaattaagat
     3421 cctttgcaga aacgtcggct tttcctgacc gccgtgtttc gcagaaaata taaaaggcag
     3481 catgacgccg gacagcagcg ctaacgccag ttgatgcgcc tgcaaaaagg cgataaaggc
     3541 caggacggca tttgctatcg gcgacagcaa atgattcagt ttgtcgaata tcacgatatt
     3601 ctccttcact taaattcggt cagcaccatc ggtcagtcag aacggtcaca ggttgtgatc
     3661 gttctgactg accgattcca tgctgcaaaa gaagtggccg ctaagcggcc atgatcgcag
     3721 gaggtacact cccgcgatct aacggcgtgc ggcattacgc gcgggctgga cgcccttact
     3781 gcatgtcgca tgggggtaat gtcctggctg aacgcccggc caaaaccccc aaggtcaaaa
     3841 gaggccacac cgtcgcgcca cgctaccggc ttttggccgg agacttaggc acgacggtgt
     3901 ggggggcaag ccccccaa
//
LOCUS       DADLSB010000256         3885 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.257, whole genome shotgun sequence.
ACCESSION   DADLSB010000256 DADLSB010000000
VERSION     DADLSB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.257"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..284)
                     /locus_tag="JN331_004050"
     CDS             complement(<1..284)
                     /locus_tag="JN331_004050"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="catalyzes the interconversion of bicarbonate and
                     carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7262005.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHII"
     gene            393..1319
                     /locus_tag="JN331_004051"
     CDS             393..1319
                     /locus_tag="JN331_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7262006.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            1316..2086
                     /locus_tag="JN331_004052"
     CDS             1316..2086
                     /locus_tag="JN331_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7262007.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            2191..2631
                     /locus_tag="JN331_004053"
     CDS             2191..2631
                     /locus_tag="JN331_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7262008.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            2695..>3885
                     /locus_tag="JN331_004054"
     CDS             2695..>3885
                     /locus_tag="JN331_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7262009.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWSQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLE"
BASE COUNT          951 a          884 c         1016 g         1034 t
ORIGIN      
        1 ataataatgt gttcaacttc gagtacatcc actgcatact gaaccacgga aaggcagttc
       61 aggtcggtgt gaatgaccag gttagcaaca ttacggtgaa caaagagttc gcccggctca
      121 aggccggtta aacgttctgc aggaacgcga ctgtcggaac atccaatcca tagaaagcgc
      181 ggtttttgcg cttgtgccag tttctcaaaa aacccgggat cctcttccac cagcattttt
      241 gaccatagtg cattgttgct gatgagtgta tctatgtctt tcatggaggt taacgacctg
      301 taaccaaata attacgtttg gctaatatag ggcaactccg ggacgattta aaccacagat
      361 aaagtgtaag aacgtaaggt aagtaaaaat ttatgaccat tgcactggaa cttcaacagc
      421 ttaaaaaaaa ctatccaggc ggcgttcagg cgcttcgtgg aatagatttg caggtcgaag
      481 cgggtgattt ttatgcgctt ctcgggccga acggggccgg gaaatcgacc actatcggta
      541 ttatcagctc tctggtaaat aaaacctccg ggcgggtcag cgtatttggt tacgatctcg
      601 agaaagatgt cgtgaacgct aaacgtcagt tgggactggt gccgcaggaa tttaacttca
      661 acccgtttga aaccgtgcag caaattgtgg tgaatcaggc agggtactac ggtgtggagc
      721 gcaaagaggc gtacatccgc agcgaaaaat atcttaagca gctcgattta tggggaaaac
      781 gcaacgaacg tgcgcgtatg ttatctggcg ggatgaagcg ccgtttaatg attgcccgtg
      841 cgttaatgca tgaacctaaa ctactgattc tcgacgaacc gaccgcaggc gtggatattg
      901 aacttcgccg ctcaatgtgg ggctttttga aggatttaaa cgacaaaggc accaccatca
      961 ttctcaccac acactacctg gaagaagcag aaatgctgtg ccgcaatatc ggcattattc
     1021 aacatggtga gctggtggaa aatacctcga tgaaggcgct gctggcgaag ctgaaatcgg
     1081 aaacctttat tctcgatctc gcaccgaaaa gtccgttacc gaagctcgat ggctatcagt
     1141 atcgactggt cgataccgcg acgctggaag ttgaagtgct acgtgagcag gggatcaaca
     1201 gcgtatttac gcagttaagt gagcagggca ttcaggtatt aagtatgcgt aacaaagcta
     1261 accgtctgga agagctgttt gtttcactgg ttaatgaaaa acaaggagat cgcgcatgat
     1321 gcatctttac tgggtggcgc taaaaagcat ctgggcgaaa gagatccatc gctttatgcg
     1381 tatctgggtg cagacgctgg tgccgccagt catcaccatg accctttact ttattatctt
     1441 cggtaaccta ataggttcgc gtattggcga tatgcatggc ttcagctata tgcagttcat
     1501 cgtaccgggg ctgatcatga tgtcggtgat caccaacgcc tacgctaacg tcgcgtcatc
     1561 attttttggt gccaagttcc agcgtaatat tgaagagctg ctggtagcgc cggttccgac
     1621 tcacgtcatt attgccggat atgtcggcgg tggcgtggcg cgtggtctgt ttgttggcat
     1681 tctggtgacg gcaatttcac tgttttttgt gccgtttcag gtgcattcgt gggtattcgt
     1741 tgccttaacc ctggtgctca cggcggtgct attctccctt gcgggtttgc tgaacggcgt
     1801 gtttgccaaa acgttcgatg acatcagcct ggtgccaacc tttgtgttaa cgccactcac
     1861 gtatttgggc ggggtctttt actcactgac tttgttgccg ccgttctggc aagggctgtc
     1921 gcacctgaac ccaatcgttt atatgatcag tggtttccgc tacggcttcc tcggtataaa
     1981 tgatgtgccg ctggtcacta cctttggcgt actggtggtc tttattgtgg cgttttattt
     2041 gatctgttgg tcgctgatcc aacgtggacg tggtttgcgt agctaaggct atttcctctc
     2101 ctctggattc gggggagagg agttttgacg gctatcaccc tttatcaaca atggtcaggg
     2161 tagactgatt ttcggctaag gaggaaggcg atgttaggtt gggtaattac ctgtcacgat
     2221 gaccgggcgc aagagatact ggatgcgctg gagaaaaaac atggggcact tcttcagtgc
     2281 cgggccgtga atttctggcg cggattaagc tctaatatgc tcagccgcat gatgtgcgat
     2341 gctctgcatg aagcggactc tggtgagggt gtcatcttct taaccgatat agccggagcg
     2401 ccaccgtatc gcgtggcttc attattaagc cacaaacact cccgttgcga agtgatttct
     2461 ggtgtcacgt taccgttaat tgaacagatg atggcttgcc gtgaaaccat gaccagttca
     2521 gagtttcgtg agcgtattgt cgaactgggt gggccggagg tgagtagtct ttggcaccaa
     2581 caacaaaaaa atccgccttt cgtcctcaaa cataatttgt atgagtatta acccgcgatt
     2641 ctgatggcgc ttttgctaca ataaaagcgt tgtttcaccc tcggttattt tttcatgtat
     2701 aaacaagctg ttattctcct gctgatgctg tttaccgcaa gtgtcagtgc cgcgttacct
     2761 gcccgttata tgcaaaccat cgaaaatgct gcggtctggt cgcaaattgg tgacaagatg
     2821 gtgaccgtgg ggaatattcg ggccggacaa atcattgccg tggagcccac tgccgcaagt
     2881 tattacgcat ttaattttgg ctttggtaaa gggtttatcg ataaagggca tctcgagcca
     2941 gttcaggggc gacaaaaagt tgaagacggt ttgggtgacc tcaacaagcc gctgagtaat
     3001 cagaacttaa ttacctggaa agatacgccg gtctacaacg cgccgagtgt gggcagtgcg
     3061 ccatttgggg tactggcgga caatttgcgc tacccgattt tgcataaact gaaagacagg
     3121 ttaaatcaaa cctggtatca gatccgtatt ggcgatcgac tggcctatat cagcgcgctg
     3181 gatgcccaac ccgataatgg cctgccggtg ctaacttatc accatattct gcgcgacgaa
     3241 gaaaacaccc gttttcgcca tacttcgacg accacctcgg tacgcgcttt caataaccaa
     3301 atggcctggc tgcgcgacag gggatacgcg acactgagca tggcgcagct ggaaggttac
     3361 gtgaagaata cgatcaatct ccctgcgcga gcggtggtga ttacctttga tgatggcctc
     3421 aagtcggtga gccgctatgc gtatcctgtg ttgaaacaat atggcatgaa ggcgacggtg
     3481 tttattgtta cctcacgcat caaacgtcac ccgcagaagt ggaacccaaa atcgctgcaa
     3541 tttatgagcg tttctgagct taacgaaatt cgcgatgttt ttgatttcca gtcacatacc
     3601 cattttttgc atcgggtaga tggttatcgc cgacccatat tactgagccg tagtgagcac
     3661 aatattctgt ttgattttgc acgttcacgc cgcgctctgg cgcaatttaa tccacatgtc
     3721 tggtatcttt cgtatccgtt tggcggcttt aatgacaaag ccgtgaaggc agcaaacgat
     3781 gccggatttc acctggcggt gacaaccatg aaaggcaaag taaaaccggg ggataatccg
     3841 ttgttactaa aacgacttta tatcttaaga acggattcgc tggag
//
LOCUS       DADLSB010000257         3865 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.258, whole genome shotgun sequence.
ACCESSION   DADLSB010000257 DADLSB010000000
VERSION     DADLSB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.258"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..512
                     /locus_tag="JN331_004055"
     CDS             <1..512
                     /locus_tag="JN331_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheinyl transferase"
                     /protein_id="HAY7262010.1"
                     /translation="SISYAGNMVGVALTTEGECGLDMELQRATRGFHSPHAPDNHTFS
                     SNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLNDDPRDLQLLPIAGRLKCAHVNH
                     VEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWKSLPDIHSRANNPTSRMMRFAQL
                     STVKSFSPN"
     gene            534..1100
                     /gene="yieF"
                     /locus_tag="JN331_004056"
     CDS             534..1100
                     /gene="yieF"
                     /locus_tag="JN331_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY7262011.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMDVIGGACCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1154..2491)
                     /locus_tag="JN331_004057"
     CDS             complement(1154..2491)
                     /locus_tag="JN331_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7262012.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            2656..3321
                     /gene="yieH"
                     /locus_tag="JN331_004058"
     CDS             2656..3321
                     /gene="yieH"
                     /locus_tag="JN331_004058"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY7262013.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            3388..3861
                     /locus_tag="JN331_004059"
     CDS             3388..3861
                     /locus_tag="JN331_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY7262014.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1029 a          966 c          973 g          897 t
ORIGIN      
        1 tttctatttc ctatgccggg aatatggttg gcgtggcgtt aacaaccgaa ggtgaatgtg
       61 gcctcgatat ggaactacag cgtgcgacgc gcgggtttca tagcccacac gcgcccgata
      121 accacacctt ttccagcaat gaatcgctat ggatcagtaa acaaaacgat cctaacgaag
      181 cgcgggcgca gctcatcacg ctgcgccgaa gcgtgctaaa actaaccggt gatgttttga
      241 atgacgatcc gcgcgatctg cagctgctgc ccattgccgg acgcctgaaa tgtgctcatg
      301 taaatcatgt agaagcgtta tgcgacgcgg aggacgtgct ggtgtggtcc gtggcggtca
      361 cgcccgcgat tgaaaagctc agtgtctggg agttagatgg caaacacagc tggaaaagcc
      421 tgccggatat tcacagccgc gccaacaatc ctaccagccg gatgatgcgt tttgcccagc
      481 tctctaccgt gaagtctttt tcgccaaatt gatagacaac cacaggagtc attatgtctg
      541 aaaaattgca ggtggttacg ttactgggga gcctgcgcaa aggctcattt aatggcatgg
      601 ttgcacgtac cctgccgaaa attgctccgg cgagcatgga agtcaatgcg ttaccttcca
      661 ttgccgacat tcccctgtat gacgctgacg tacagcagga agaaggtttt ccagcaacgg
      721 ttgaagctct ggcggaacag atccgtcagg ctgacggtgt ggtgatcgtc acgccggaat
      781 ataactactc ggtaccgggt gggctgaaaa atgccatcga ctggctttcc cgcctgccgg
      841 atcaaccgct ggccggtaaa ccggtgttga ttcagaccag ctcaatggac gtgattggcg
      901 gcgcgtgctg tcagtatcac ctgcgccaga ttctggtttt cctcgatgca atggtgatga
      961 acaagccgga atttatgggc ggcgtgattc agaacaaagt tgatccgcaa accggagaag
     1021 tgattgatca gggtacgctg gaccacctga ccgggcaatt gaccgcattt ggtgagttta
     1081 ttcagcgagt taagatctaa ataaaaaacc cgccagtaat catgcatggc gggtttttaa
     1141 tgcgctatcg attttaatga gcgtcgataa atacaatctt caggataaac agcagcgcaa
     1201 cgatgattac gcacgggcta aggtcacgca gacgcccggt accaatcttc atcacgcagt
     1261 aggagataaa gcccagcgca ataccttcgg taatcgaaaa gctgaacggc atcatcacgg
     1321 cggtaataaa cgccggaaca gattcagtaa gatcctgcca gttcacgcga gcaagacttg
     1381 aggtcatcaa cacgccaacg tagatcaacg cgccagctgc agcgtagcct ggcaccattc
     1441 ccgccagcgg cgacagaaag ataaccagca ggaacagcag accaacaacc actgccgtca
     1501 gaccggtacg accgccaacc gatacgccgg aagaggactc aatataagcc gtaacggaag
     1561 aagtaccgat aaacgaaccg gtcacggaag agatactgtc gacatacagc gcctgcttca
     1621 tgcgcgggaa tttccccttc tcatccgcca gacctgcttt atcggtcacg ccaatcagcg
     1681 taccggagga gtcaaacaag ttgaccaaca tgaaagagaa aatcacccct gccagcccga
     1741 ggttaaacga cccggctaaa tctacatgac ccacaactgt cattacgctc ggcggtgcag
     1801 aaacgatgcc attgtagtgc acatcaccca gcatccagcc cagcagcgtc gtcaccacga
     1861 tagaaaccag caccgctgcg tgaatgttgc gcgaggccag aatagcaatg atgaaaaagc
     1921 cgaggatacc cagaagtacg ctgtgagaag tcagattacc gatgctcacc agcgtttccg
     1981 agttagcgac aatcacacct gcgtttttca gccccatcat gccgataaac aggccgatac
     2041 cgctggtaat acccacacgc agacttaccg gaatgttagc aatcatccag taacgaacgc
     2101 ggaaaatggt caaaagaagc agtccgacag cgccccagaa gattgcgccc ataccgatct
     2161 gccacggcag ccccattgcc tggacaacca caaaagcgaa aaaggcgtta agccccatcg
     2221 caggagccag cgccaccgga aggtttgcaa acaatcccat cataatgcta ccgaaagcag
     2281 caatcagaca ggttgttacg aagacggcac tggtgtccat gccagcaacg cccagaattt
     2341 gtgggttaac aaatacgatg tagaccatcg tcaggaaggt ggtgaacccg gcaatcactt
     2401 cagttcgtgc cgtagtacca tgttcgcgca gtttaaaaac gcgttccagc atcctctgac
     2461 cagaagtctg ggtggtatgt tgatgactca ttatctattt ccgaacaagg agggaaaatc
     2521 cgtcgctatc gtataccaaa atgcgacaat aggtgcgttt gtgagagact tttttattgg
     2581 atttacttat acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga
     2641 ttaaaaagga aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg
     2701 gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt
     2761 acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc
     2821 gatattgttt cccttgaaca tggtgttacg ttagcgaaaa cagaagctga acacgtttac
     2881 cgtgcagaag tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctggagcg
     2941 ctcctgtcag cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg
     3001 cagcattcta tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc
     3061 tacgatattc agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg
     3121 aatgtaaatg tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt
     3181 atcgacgcag gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt
     3241 cacccgaaag tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt
     3301 ggttgggata ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg
     3361 ctcaaaggta agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga
     3421 ctttattttg cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc
     3481 ttcgcaaaag aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta
     3541 ttgtcgatta ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg
     3601 ccggaaaaga tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc
     3661 gttatcaccg agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag
     3721 ttgtttgaaa acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg
     3781 gcaggtgtgg tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct
     3841 gaaacagact ctttaagtta agcgg
//
LOCUS       DADLSB010000258         3855 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.259, whole genome shotgun sequence.
ACCESSION   DADLSB010000258 DADLSB010000000
VERSION     DADLSB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.259"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            343..540
                     /locus_tag="JN331_004060"
     CDS             343..540
                     /locus_tag="JN331_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY7262015.1"
                     /translation="MNAHDIEPTILRATGNTHPLDVTFIHEEDLATPWKPEQSSVYAH
                     VNPYGIFELDMETRLPIEVVA"
     gene            complement(622..2331)
                     /locus_tag="JN331_004061"
     CDS             complement(622..2331)
                     /locus_tag="JN331_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY7262016.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="JN331_004062"
     CDS             complement(2764..3483)
                     /locus_tag="JN331_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY7262017.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3608..>3855)
                     /locus_tag="JN331_004063"
     CDS             complement(3608..>3855)
                     /locus_tag="JN331_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7262018.1"
                     /translation="WQRWLSLAANSDVPMMKNVAKTIGKRLYGILNAMRHGVSNGNAE
                     ALNSKIRLLRIKAKGYRNRERFKLGVMFHYGKLNMAF"
BASE COUNT         1119 a          800 c          699 g         1237 t
ORIGIN      
        1 agcttgatac aactggtctg gtatatacag tttctttgag tgcggttacg tccacagagg
       61 taacggtaaa agtaacccct tgtcgggacc actccgaccg cgcacagagg cattttcatc
      121 gctgacaggc cgcattgcct cgcagggctg gaggaggatc ggtgatatcc ggacgaccgt
      181 accggcttac gccgggcttc ggcggtttta ctcctctgcc atgtgcaaca acggagtgcc
      241 gccttacatg ccgctggcgc ggcatatcct ggtaacattt ttgttgatac caccttccgg
      301 ccttatcctg accagtgggc tttcctggca tcgatgatac cgatgaacgc gcatgatatt
      361 gagccgacga tattacgggc aactggaaat actcaccctc tggatgtgac ctttatccat
      421 gaagaagatt tggccacacc atggaaacct gaacaatcat cagtttacgc ccatgtaaac
      481 ccctacggga tatttgaact cgatatggaa acaaggttgc caatcgaggt ggttgcctga
      541 aagttggcag ttacccggtc actacacctt gttggagtga acatatcaaa aacaggtaat
      601 tacacaattt aaattacagt ctcataaaaa atgattaaat ctatttatta tttcaataaa
      661 cgtttttttc atcttctttg aaaattgttc tgctatagta tccggttcca gattgttctc
      721 ttttattatt ctacaatact ctaatattgt ttctttgtct ctatttacta cagatacaaa
      781 tacaccagga ataccatctt tattagttgc ggacagcatt tcgaccaatc gtcttgtggt
      841 aaaactatag ttttttagaa tattacaaaa ggcacgtatt gtttcggtat ggccattata
      901 aaatgccata aaaagtcctg atgtaccgtt gggatttttt atccataata attcatctat
      961 atattcctgg tgagggggga gtatttttag tatatctcca tacgccataa tggcctttgt
     1021 gtgcccattt tgaagtgcca taaacaaccc atgctttact ttcaataatt ctttaatttg
     1081 ttcactacta agcattttct tattaagcag acttccgaac atacgaatac tatctgcatg
     1141 tccattctgc aatgcaaaaa ataacccagg taaatcatgg ctgtctttcg cagctaatag
     1201 atataatctc ttatcggaag ttaacggagt tttaaagact atttccccat aggcttttat
     1261 tgtgtctaca tgtccgttca taaaagacat gcataaaccc ggcacattgt ttgcccctac
     1321 cgcagcaagc aatgtaagta ttgtttcttg actaagattg cacatattta atatcttacc
     1381 atatacttga ataatgtctg aacatccgtt ttgaagtgct atataaaatg caggagtacc
     1441 atcagagctt ctcgtggcca gaaggttcac cagtttagat tgagaaattt caccattttt
     1501 gtatttttcc atcagtataa ataatacttc tgtcagattc tttgttatcg catgatatat
     1561 aactctggaa ttgatcaatt catctggtaa aagtttcact gtgtttgtta gaggggaatt
     1621 atgcctatct tcaaatatta cctgcccggg ttgctccaga ccataaattt tccaataaaa
     1681 atcatcaaaa aaatccttag cggataattg actcagttcg aaatggctat cacaagtaaa
     1741 tacagtacgt ttatgagtaa cagttcgatt aggatcgaaa aactgaacaa cccatttttc
     1801 actttcttgt acgcgcttta ttttcaaccc taacgccatt tggtggttta atgtgcatgc
     1861 tgataatgtt ctgacaaagt ctccattttt tttcatgtta taaaaaaaat tagtaataac
     1921 tgttccccac tttttatttt ccacaaaata aatataagct ggtgattttg aaaaatcttc
     1981 cgttttttcc aaacatacat tttttgcaat agcatcaggg gtgctaagaa gctgatagtt
     2041 taccttccca tttgtttcca taaaattcat ttcccaatag gaagctaggt gacggcaaac
     2101 tatttgtctt ccatatatat cttttgccac tccatttata tttaccttca catttctctt
     2161 ttctgaaaaa taaggtgctg ttttattctt aaactgaatt agttcactgg agtttgagac
     2221 cctcataata gaacttgtta ttgttctatt atctctgtta acagacatat ctgttgataa
     2281 cgaatttttt gttgaaaata tactagatga aaatgtttta tttaacggca tagaagacaa
     2341 ccatagagat gttataatac tattagattg cagttactaa tagtaaaagc ctccttacaa
     2401 ggaggtagct tattcagaat gctcgctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaataa cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgactagct
     2641 cagcagttta tgagccgaat gctctgtcac gggcaggaat caggctgcat acgctgtgcc
     2701 aaatctaatc gtagttattc taaccagttt tcaagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tactctgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttctcc gatcatggtg
     3601 ggaaggctta gaacgccata ttcagtttcc catagtggaa catcactccc agcttaaagc
     3661 gctcccggtt gcggtagccc ttggctttta ttctcagcag cctgatcttg ctgttcagtg
     3721 cttccgcatt tccgtttgat acgccatggc gcatggcgtt caggataccg tacaaccttt
     3781 tccctatcgt tttagccaca ttcttcatca tggggacgtc actgttggcc gccagcgaca
     3841 gccatctctg ccagt
//
LOCUS       DADLSB010000259         3839 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.260, whole genome shotgun sequence.
ACCESSION   DADLSB010000259 DADLSB010000000
VERSION     DADLSB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.260"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..458)
                     /gene="intS"
                     /locus_tag="JN331_004064"
     CDS             complement(<1..458)
                     /gene="intS"
                     /locus_tag="JN331_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY7262019.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(620..694)
                     /locus_tag="JN331_004065"
     tRNA            complement(620..694)
                     /locus_tag="JN331_004065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(659..661),aa:Arg,seq:cct)
     gene            complement(770..1702)
                     /locus_tag="JN331_004066"
     CDS             complement(770..1702)
                     /locus_tag="JN331_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAY7262020.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTPHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1996..2751
                     /gene="mlaA"
                     /locus_tag="JN331_004067"
     CDS             1996..2751
                     /gene="mlaA"
                     /locus_tag="JN331_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY7262021.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2933..3763)
                     /locus_tag="JN331_004068"
     CDS             complement(2933..3763)
                     /locus_tag="JN331_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262022.1"
                     /translation="MDIGSNDTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEK
                     THDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLFTWYGKD
                     KKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSENARSASAWFE
                     SSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVK
                     EIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQAKYQNEINQLSALGVNFDDI
                     "
BASE COUNT          960 a          876 c          855 g         1148 t
ORIGIN      
        1 gcaccgcgat cttcaaatct gcggattact tccagcagtt gcatcggctc aatatcctga
       61 atctcaaggc cgccgatgat gggtaaaatg tcgtcatcaa acatttttgc aagttcagtc
      121 gcatacccta ctgaccagac ttgcttcttg tgttcgtacc attccttgta aattgcacta
      181 aatgaattgt tgttagacga agcctttttc gcctttaccg gatcgatgcc agccgagatg
      241 tctttcctcg cagtccatgc tttatccctt gcctcctgca aagtcataag cggatatttt
      301 ccgacggtca ggattttctc cttaccgtca atcttgtagc gaagctgcca tacctttttc
      361 ccggatacag ggacataaag gtacaggcca ttaccatcga gaaggcggta tggtttttct
      421 ttcggctttg ctgcttcaat ctgcttaacg gtgagcatgg gtaaaaatcc ggtgggtaaa
      481 attattttat ccacttttta cccgtcatgg agtgcggctg tcaacgatct gacgcgaacc
      541 atgacgaact gtgaatatac ggaaggcttg atattcaggg gattttgcgg actggtacgg
      601 atgggagcga actgataaat ggtgtcccct gcaggaatcg aacctgcaat tagcccttag
      661 gaggggctcg ttatatccat ttaactaaga ggacaatgcg gcatgagtat acccgctaat
      721 ggactgcggg gtaagtacgc tgccgctcga ttgcttaaac cctcgccatt tatgctgggt
      781 ttttatcatt tttcttaatg ttttccgcac gttctgcttt ttggcgtgct tctgctttac
      841 gcttgttgct catgtcgtta cgaatctgtg catgactcat taacgcgaag ataaaggtgc
      901 cgccgcagat gttccccgct aaagtaggta gtgcgaaggg ccagatgaaa tcgctccagt
      961 gcagcgtacc gttaaacacc agatagagga tttcaacaga accgaccacg atatgggggg
     1021 tgtcacccag ggcaataagc caggtcatca atataatcac cacaatcttt gccgcacctg
     1081 ctgcaggaaa catccatacc atagtggcga tcagccagcc ggaaatgatc gcgttggcaa
     1141 acatctcgct gggggtgttc ttcatcacat ccatgccgat tttgacaaat gcatcgcgag
     1201 tttcttcatt gaagataggc atatattcaa atgcccacgc agcaatacca gtcccgagaa
     1261 tattacccag cagcacgact ccccataacc gcataagtaa gccaacgttg ctcattgtcg
     1321 gtttttgcat gacgggtagt accgcagtca cggtgttttc ggtaaataat tgctggcggg
     1381 ccattatgac gataataaaa ccaaaggtat aaccgagatt ctccagcaag aagctgcccg
     1441 gcacaccttc cagttcgaca tgaaatatcc cttttgccag taacgaagcg cccatcgaca
     1501 gacccgccgc aatggctgac cacagtagcg ccattgcgtc gcgttctagc tctttttcac
     1561 catcctggcg gatatgctca tgaattgcca tcgcccggga ggggagtcgg tcttcatcta
     1621 tttctatttt tttgccgcgc tctttttctt cgctctcaac ttcaatttcg tcgctgtgtt
     1681 gatcaatttt gtcgttgtcc atggtctctt ctcttgaatt agcacgtata gctaaagcgt
     1741 agcggctttt ttgctcgcaa ctggcgggag ttactctgaa aatgtagaaa aggctgcgtt
     1801 tgcctttttc tgtttctata gaatcaagta gcctacaggg cggcgattac caggctatga
     1861 tcaaatcagc aaatcagggc gtctggacat cagttgacgt gctgttacaa tcgcccacac
     1921 ctaaacaggc ggatacggta tcgttccgtc atggatggca aactgcataa gccataaaaa
     1981 aacagggaga catttatgaa gcttcgcctg tcggcgcttg ctctgggaac tacgcttctg
     2041 gtggggtgtg cgagttccgg tacagatcag caagggcgtt ctgacccgtt agaagggttc
     2101 aaccgcacca tgtacaactt caacttcaac gtattagacc cgtatattgt tcgaccggtc
     2161 gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta atggtttgag caactttact
     2221 ggcaaccttg aagaacctgc ggtgatggtt aactacttct tgcagggcga cccttatcag
     2281 gggatggtcc actttacccg ctttttcctg aacaccattt tggggatggg cggctttatt
     2341 gatgttgcag ggatggcgaa cccgaaactg caacggactg aacctcaccg cttcggtagt
     2401 acgcttggtc attatggcgt gggttatggg ccttacgttc agttaccgtt ctacggtagc
     2461 ttcacgctgc gtgatgacgg tggtgatatg gcggatggtc tttacccggt tctttcctgg
     2521 ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag ggatcgaaac ccgcgctcag
     2581 ctgctggatt ccgatggtct gctgcgtcag tcgtccgatc cttatattat ggtgcgcgaa
     2641 gcgtacttcc agcgtcatga tttcatcgct aatggcggcg aactcaaacc gcaggaaaat
     2701 ccgaacgcgc aagcgattca ggatgattta aaagatatcg attctgaata agaaacaaat
     2761 aaaaaaggtg agtcgcaaaa ctcacctttt ttgttgttat ccctcaatta ccacaagtaa
     2821 aaggagataa accgacaatg acccggacgt aatgatgtaa aaatactgat gttcgtcact
     2881 gactatttcc gatattcagg cgttgaaaaa tcaaaaaact ggaaaaataa ttttaaatat
     2941 catcgaaatt aacacctagt gcagatagtt gattaatttc attttgatat ttcgcctggc
     3001 gattttgaag tcttttttca actttatctt cgagatcctt aaagtctttg tatttatttt
     3061 caggatggtc aacgtcaact ttgttagcga ataaagtaga aattatttct tttacattat
     3121 cgcgtctaaa ttttgcatcg tccacttgaa aattatatct tattgctcga cctaaacgtt
     3181 taaaaacttc gttaggttct ctcagtcttt ccccgttgag ctcgtttaca acctgatgat
     3241 cggaaccagt tgcaaatgat tctgccatga tagctttact gctttcaaac cacgctgaag
     3301 cactcctggc gttttcacta caatattggg tgagactatc accattaata cgaattcctc
     3361 tttgaatgtg gtagtcgtcc tgatcgtagt aaccgagaga taagtaatca tttccgtttt
     3421 tatttttaat tacaatagcg agagagtcgc cttttttatc tttaccgtac caggtaaata
     3481 agtccgttgg ttcgcttttc ccgctctcat caaattccgt taccgtatgt ctgagtttgt
     3541 gtgatatctt atcgaagata aaaccaggat ctccatcata tatttctttt aatgcgctga
     3601 agatcatttc cttgttattg atatcatgag tcttctcaac tatgttgtca ttaatgatag
     3661 tacggacgag ttctttgtct ttatagttcg caggtaattc tattggttct tggttgtcga
     3721 taccaatagt aatatggtct gtatcattgg agccaatatc aatataagta actgaggaat
     3781 tattaatatt aaatctataa ggatcgtcgt ttactcgtat ggcgtttttt ataagctca
//
LOCUS       DADLSB010000260         3794 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.261, whole genome shotgun sequence.
ACCESSION   DADLSB010000260 DADLSB010000000
VERSION     DADLSB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.261"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..479)
                     /gene="fimB"
                     /locus_tag="JN331_004069"
     CDS             complement(<1..479)
                     /gene="fimB"
                     /locus_tag="JN331_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
                     /protein_id="HAY7262023.1"
                     /translation="MRNKADNKKRNFLTHSEIESLLKAANTGPHATRNYCLILLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     "
     gene            1933..2649
                     /gene="nanC"
                     /locus_tag="JN331_004070"
     CDS             1933..2649
                     /gene="nanC"
                     /locus_tag="JN331_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY7262024.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2669..3775
                     /locus_tag="JN331_004071"
     CDS             2669..3775
                     /locus_tag="JN331_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY7262025.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSYAPMGMAGHVTFVHNGKAYVTGGVNQNIFNAYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
BASE COUNT         1188 a          750 c          778 g         1078 t
ORIGIN      
        1 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatggcg taacatgtgc
       61 ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac
      121 tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc
      181 ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc
      241 gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga
      301 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
      361 agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt
      421 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
      481 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
      541 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
      601 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
      661 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
      721 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
      781 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttcaac tagccgtctc
      841 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
      901 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
      961 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
     1021 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
     1081 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
     1141 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
     1201 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgggta
     1261 aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct
     1321 aaataaattt tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
     1381 caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt
     1441 gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
     1501 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
     1561 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
     1621 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
     1681 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
     1741 atctggatct ctgtttattt aaataatgtg aaaagagaat tttcacagga gaccttatac
     1801 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
     1861 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
     1921 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
     1981 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
     2041 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
     2101 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat
     2161 gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg
     2221 ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc
     2281 tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac
     2341 cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat
     2401 gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa
     2461 cagaatgatt atcgctatgc aaaccacaag aaatgggcaa cggaaaatgc ctttgttcta
     2521 caataccata tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag
     2581 ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt
     2641 aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat
     2701 gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag
     2761 tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc
     2821 atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc
     2881 tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg
     2941 cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa
     3001 ccccaaaacc aatagctggg ttaaattgat gtcgtacgcg ccgatgggca tggcgggtca
     3061 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt
     3121 caatgcctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat
     3181 caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc
     3241 ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc
     3301 tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc
     3361 aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa
     3421 taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa
     3481 tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa
     3541 cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg
     3601 gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc
     3661 attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt
     3721 gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt
     3781 cagggccccg gtaa
//
LOCUS       DADLSB010000261         3782 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.262, whole genome shotgun sequence.
ACCESSION   DADLSB010000261 DADLSB010000000
VERSION     DADLSB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.262"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1064
                     /gene="aldA"
                     /locus_tag="JN331_004072"
     CDS             <1..1064
                     /gene="aldA"
                     /locus_tag="JN331_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7262026.1"
                     /translation="IQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTG
                     NTIVIKPSEFTPNNTIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMT
                     GSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCN
                     CAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAALERVEQKVARA
                     VEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAIS
                     MANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGA
                     DGKHGLHEYLQTQVVYLQS"
     gene            complement(1106..2107)
                     /gene="gap"
                     /locus_tag="JN331_004073"
                     /pseudo
     CDS             complement(1106..2107)
                     /gene="gap"
                     /locus_tag="JN331_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            2296..2826
                     /gene="cybB"
                     /locus_tag="JN331_004074"
     CDS             2296..2826
                     /gene="cybB"
                     /locus_tag="JN331_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY7262027.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3071..3244
                     /locus_tag="JN331_004075"
     CDS             3071..3244
                     /locus_tag="JN331_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262028.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3316..3465)
                     /gene="hokB"
                     /locus_tag="JN331_004076"
     CDS             complement(3316..3465)
                     /gene="hokB"
                     /locus_tag="JN331_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY7262029.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(3565..>3782)
                     /locus_tag="JN331_004077"
     CDS             complement(3565..>3782)
                     /locus_tag="JN331_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7262030.1"
                     /translation="RKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEY
                     YNSRRISLKLKGLTPNEYRNQTYMPRV"
BASE COUNT          978 a          853 c          930 g         1021 t
ORIGIN      
        1 ttattcaaag cgatcgtcca ggagaaaata ttcttctgtt taaacgtgcg cttggtgtga
       61 ctaccggcat tctgccgtgg aactttccgt tcttcctcat tgcccgcaaa atggctcccg
      121 ctcttttgac cggtaatacc atcgtcatta aacccagtga atttacgcca aacaatacga
      181 ttgcattcgc caaaatcgtc gatgaaatag gccttccgcg cggcgtgttt aaccttgtac
      241 tggggcgtgg tgaaaccgtt gggcaagaac tggcgggtaa cccaaaggtc gcaatggtca
      301 gtatgacagg cagcgtctct gcaggtgaga agatcatggc gactgcggcg aaaaacatca
      361 ccaaagtgtg tctggaatta ggtggtaaag caccggctat cgtaatggac gatgccgatc
      421 ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat taatagtggg caagtgtgta
      481 actgtgcaga acgtgtttat gtacagaaag gcatttatga tcagttcgtc aatcggctgg
      541 gtgaagcgtt gcaggcggtt caatttggta accccgctga acgcaacgac attgcgatgg
      601 ggccgttgat taacgccgcg gcgctggaaa gggttgagca aaaagtggcg cgcgcagtag
      661 aagaaggggc gagagtggcg ttgggtggca aagcggtaga ggggaaagga tattattatc
      721 cgccgacatt gctgctggat gttcgccagg aaatgtcgat tatgcatgag gaaacctttg
      781 gcccggtgct gccagttgtc gcatttgaca cgctggaaga tgctatctca atggctaatg
      841 acagtgatta cggcctgacc tcatcaatct atacccaaaa tctgaacgtc gcgatgaaag
      901 ccattaaagg gctgaagttt ggtgaaactt acatcaaccg tgaaaacttc gaagctatgc
      961 aaggtttcca cgccggatgg cgtaaatccg gtattggcgg cgcagatggt aaacatggcc
     1021 tgcatgaata tctgcagacc caggtggttt atttacagtc ttaatgagtg aaagaggcgg
     1081 aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa tttttcgagg gtgcgaataa
     1141 gctgcgtgac gaagccatat tcgttatcgt atcaggcgac cgttttcacc agttgtaaat
     1201 cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac tgaaccgaaa tggctgccaa
     1261 tgatatcgga agagactatt tcttcatcgg tataaccaaa tgactcgtta ttggtggttg
     1321 cttgtttaag tgcgttattc acctcttcgg cagtcacttt ttttccgaga atggacacca
     1381 gctcagtgac cgaacctgtt ttcaccggca cgcgttgcgc atgacctttc agtttgccgc
     1441 tcagttccgg gatcaccaga ccaatggctt ttgccgcccc cgtagtgtgg ggaatgatat
     1501 tttctgccgc tgcgcgtgaa gcacgtaaat ctttaccacg tgggccatcc accagtgact
     1561 gggtgccagt ataggcatga atggtcgtca tcgtgccgac ttctatcccg aaactgtcat
     1621 gcaaggcttt ggccatcggc gcaaggcagt tagtggtgca tgacgccacg gaaacaatgg
     1681 tgtcgttgcc atccagagtg tcgtcattaa cgttataaac gatggttttc atttcaccgg
     1741 caggagcgga aatcaacacc ttcttcgcac cagcatcaag atgcgcctgc gatttatcgg
     1801 cggaggtata aaagccagta cattcgacaa tgatttctgc ccctttcgct ttccacggaa
     1861 tatttttagc ctctttttcg gcgtaaaccg cgatactttt cccatcaacg ataagtgaat
     1921 cttccgtaaa atcaacgctc caggggaacg gaccatagtt tgaatcatgt ttcagcagat
     1981 aggcgagaat ttttggggaa gtaagatcat taatagcgac aacgtctatg ttgcttttga
     2041 cttcaagtaa acgacgcaac accagtcgac cgatacgacc aaaaccgtta ataccaactt
     2101 tactcatggt tttctcctgt caggaacgtt cggatgaaaa ttgatccttt ccaggcttag
     2161 accaggattg tgggatgggc aatctccatt cttacagtga aacgtaacgt actgaaaacg
     2221 ggtgaacaat atttaatgaa attttgagaa aagcccgtta tgttaacgga aaattatgtt
     2281 aaagcaggaa atgttatgga aaataaatac tcaaggttac aaatcagcat tcactggctg
     2341 gtctttttgc tggttatcgc agcgtattgc gcaatggagt ttcgtggttt cttcccacgt
     2401 agcgatcggc cgctcatcaa catgattcat gtttcctgtg gcatctcaat tctcgtgctg
     2461 atggtggttc gtcttttgtt aagactgaaa tacccaaccc cgccgattat acctaagccg
     2521 aaaccgatga tgacgggact ggcgcatttg ggacatttgg tgatttatct cctgtttatt
     2581 gcgctgccag tgattggttt ggtgatgatg tataaccggg gcaacccgtg gtttgcgttt
     2641 ggtttgacga tgccttacgc ttcagaggcc aatttcgaac gggttgatag cttaaagtcg
     2701 tggcatgaga cgctggcgaa tctgggatat tttgtcatcg ggttgcacgc tgcggctgca
     2761 ctggcacacc actatttctg gaaggacaac acacttctac gcatgatgcc gcgtaaacgt
     2821 tcctgaagga tatttaaaga aaacgcctgt actaaaaccg acccgtggta caggcgaaga
     2881 atacgggtct acatcggaag cgcctattat atttatttgt atgacaaata aaaccataat
     2941 ccttgcccat acgtccatct ggcttatttt taatcaattc acccgttctt tgatctcatc
     3001 aacggtatca aaataaacca gcgtataacg ttcaaatcgc acataagatt cgataaacca
     3061 tggaggttat atgaaaaaac tggcacttat tttgtttatg ggaacgcttg tttcctttta
     3121 tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg gggatctcac actgtacggc
     3181 aggcggcaaa tttgtctgta atgatggttc tattagtgca tcgaaaaaaa catgcactaa
     3241 ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct agtcagatgc gcggggaaaa
     3301 tccccgcgct tgcccttacc tggacgtgca ggccatgagc gcagcaacct ccttatcacc
     3361 gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg agtgtgaatg ccagaatcgt
     3421 aatgcagata atgagcagac acaccaccag agggttgcgc ttcatagcct ttctccttgc
     3481 cggatggcgg gtaagaggct aacatctgaa ttgcttggtt cattcgttgg cctcggtttg
     3541 tactgacccc aaaaagttgg acagttaaac acgaggcata taggtctgat tccgatattc
     3601 atttggagtc agacctttta atttcaggct aattcttctg ctgttgtagt attcaatata
     3661 ttccgtaaca gcatccttca gttcgcttat attactgaac tcatcaagat aaaaacactc
     3721 cgactttaag gttccaaaga aacactccac cacagcatta tccagacaat tgccttttct
     3781 gg
//
LOCUS       DADLSB010000262         3758 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.263, whole genome shotgun sequence.
ACCESSION   DADLSB010000262 DADLSB010000000
VERSION     DADLSB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.263"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(281..487)
                     /locus_tag="JN331_004078"
     CDS             complement(281..487)
                     /locus_tag="JN331_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY7262031.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            800..877
                     /gene="kdpF"
                     /locus_tag="JN331_004079"
     CDS             800..877
                     /gene="kdpF"
                     /locus_tag="JN331_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009651669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY7262032.1"
                     /translation="MSAGVLLVFLLLGYLVYALINAEAF"
     gene            877..2526
                     /gene="kdpA"
                     /locus_tag="JN331_004080"
     CDS             877..2526
                     /gene="kdpA"
                     /locus_tag="JN331_004080"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY7262033.1"
                     /translation="MAAQGFLLIATFLLVLMVLARLINDIPLPGTAGVERILFRLPGV
                     SDHEMNWKQYLCAILGLNMLGLAELFFMLLGQHYLPLNPQQLPGLSWDLALNTAVSFV
                     TNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSMSTLGNAWV
                     DLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGPVASQEAIK
                     MLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGDRRQGRMLL
                     WAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSLFAVVTTAA
                     SCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVFIAGLMIGR
                     TPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGPHGFSEVLY
                     AVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLVSKKSQPAS
                     SGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2549..>3758
                     /gene="kdpB"
                     /locus_tag="JN331_004081"
     CDS             2549..>3758
                     /gene="kdpB"
                     /locus_tag="JN331_004081"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HAY7262034.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMHGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRR"
BASE COUNT          804 a          926 c         1047 g          981 t
ORIGIN      
        1 atgacggctt tctcacctta ccctgaagtt tcacgaaagt gtgtaaggaa gtatgcaatc
       61 taactggaga tttctgatga tctagtcgct tagagcgaca atgaaggtga ccgcatcctg
      121 ggtagcccta tgaaaggata ctattgaaac gtgatgctaa aacagtgatg aaatgaataa
      181 tacgatggaa acgtcttcca tgttaattat catggaagac gttgatgttg aacacctaaa
      241 ccagccccct gatacgaggc tggcttaagc gcgcaggcaa tcagtaaaaa tcaccggttg
      301 ccttttcggc ctgatccatc cacaccggtt tatcgctggt tttcgaccag acgcggtgca
      361 ggtagctgta aaagcgggcg cggtctttcc agaaaagcat gaaaggcagc gccagaacgc
      421 ccgctgcaac cgcatatgta cggcgtaaaa agataagcca tgcaggatat tctctgtaga
      481 gttccatact tttctccccc ttttgtatct acccggtgaa tagcaccgga aaaatgaatt
      541 tgtttatctg atgaaaatag taccgccttt tgtgtaattt tactactcat ccgaccactt
      601 atttttgctt attgatggtt tatttacatt cacactgtaa ttaaattaca caaaagttaa
      661 attaatacta accatcagtt aaatcatggc ctttgccatt tttatacttt ttttacaccc
      721 cgtccgcaga tttttgcgaa atctttgcag ccagaattct actcttccgg tatcactttt
      781 aggccactgg aggtgcactg tgagtgcagg cgtgttgctg gtgtttttat tactgggtta
      841 tttggtttat gccctgatca atgcggaggc gttctgatgg ctgcgcaagg gttcttactg
      901 attgccacgt ttttactggt attaatggtg ctggcgcggc tgattaatga cattcctctt
      961 ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc tgaccatgag
     1021 atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg gctggcggag
     1081 ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca gttgccgggg
     1141 ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac caactggcaa
     1201 tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt aacggtacaa
     1261 aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg tgcgtttact
     1321 cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat cacgttatgg
     1381 gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg tgcgctgcaa
     1441 aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct gttacccatg
     1501 ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg tggcttcttt
     1561 aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt cgtgcagatg
     1621 ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt gacgggcgat
     1681 cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat ctgcgtaggc
     1741 gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg cgcagacagc
     1801 agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag cctgtttgcg
     1861 gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc gtttaccgct
     1921 ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt cggcggtgtc
     1981 ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat tgccgggctg
     2041 atgattggtc gtacaccgga atatctgggt aaaaaaatcg acgtacgcga gatgaaactg
     2101 actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc gttggcgatg
     2161 atgaccgacg ccggacgtag cgccatgctt aaccctggcc cgcatggttt tagcgaagtg
     2221 ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg attaagcgcc
     2281 aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg cttcggggtg
     2341 attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca acccgccagc
     2401 tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg caccgtgttg
     2461 ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc ggaatatctc
     2521 tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc tattcgaacc
     2581 aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc aggcgcaatg
     2641 gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct gtattagcat
     2701 cgcgatggca agcggtgcga tgcatggcaa tgcgctgttt agcgcggcaa ttagcggctg
     2761 gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag gccgcagtaa
     2821 agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca agctgcgtga
     2881 gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta aaggtgacat
     2941 cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg aaggcggcgc
     3001 atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg aatccggcgg
     3061 cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg tgattgagtg
     3121 tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg aaggcgcaca
     3181 gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc tgaccatcgt
     3241 ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta atgccgtgag
     3301 tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg gtggcctgtt
     3361 gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga ttgccaccag
     3421 cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata aaaccggcac
     3481 catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg tggatgaaaa
     3541 aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg aaggtcgcag
     3601 tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc agtcgctcca
     3661 tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca tcgacaaccg
     3721 catgatccgt aaaggttctg tcgatgccat tcgccgcc
//
LOCUS       DADLSB010000263         3724 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.264, whole genome shotgun sequence.
ACCESSION   DADLSB010000263 DADLSB010000000
VERSION     DADLSB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.264"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(5..385)
                     /gene="panD"
                     /locus_tag="JN331_004082"
     CDS             complement(5..385)
                     /gene="panD"
                     /locus_tag="JN331_004082"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY7262035.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERSSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            659..1561
                     /locus_tag="JN331_004083"
     CDS             659..1561
                     /locus_tag="JN331_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY7262036.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            complement(1635..2486)
                     /gene="panC"
                     /locus_tag="JN331_004084"
     CDS             complement(1635..2486)
                     /gene="panC"
                     /locus_tag="JN331_004084"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY7262037.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2498..3292)
                     /gene="panB"
                     /locus_tag="JN331_004085"
     CDS             complement(2498..3292)
                     /gene="panB"
                     /locus_tag="JN331_004085"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY7262038.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     ERLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(3405..>3724)
                     /locus_tag="JN331_004086"
     CDS             complement(3405..>3724)
                     /locus_tag="JN331_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7262039.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
BASE COUNT          897 a          917 c          962 g          948 t
ORIGIN      
        1 acaatcaagc aacctgtacc ggaatcgctt tcgcggtacg tttcatttca ttgtcgcctt
       61 caaaataggc gacattgggt cgccaggtgc gagcttcttc atctggcatg gtaacgaagc
      121 tggcgatgat gacaatatcg ccgacgctgg cgcagtgggc cgccgcaccg ttaacagaaa
      181 taattctcga actgcgttct gccgcgatgg cataagtgga gaaacgcttg ccgttggtga
      241 cattccagat atcgatggct tcgttttcga gaataccggc tgcgtcaaga aaatcctggt
      301 caatggcgca agaaccttca tagtgcaggt ccgcatgagt cactttcacg cggtggagtt
      361 tgccctgcag catcgtgcga atcataactt ctacctttct accctgtcgt taacgaagca
      421 ggcggtgcct gctttgagga aattctcacg cagtattgcc cgatttttga tgagtgtcta
      481 ctcatctgat ggcatttgcg tcagcagttt gcgtaccgcg ccgaagcgtg ccatgtaacg
      541 cctgtaacga attgatattt ttatgaacgc tgctcggtaa acaataaaga ggtggctgac
      601 gatagcacgc cttgactgga tactgccttc acgcaaggcc acacggaaga cagggaagat
      661 ggatgcaccg agtaccacac cgcatgacgc ggtatttaaa caatttttaa tgcatgcgga
      721 gacggctcgc gactttctgg agatacattt gccagtggaa ttacgcgaac tttgtgatct
      781 caacacgctt catttagagt cggggagttt cattgaagag agcctgaaag gtcacagcac
      841 ggacgtgctc tattccgtgc aaatgcaggg caatcccggt tatctgcatg ttgtgattga
      901 acaccaaagc aagccggata agaaaatggc ctttcgcatg atgcgttatt ctattgctgc
      961 catgcaccgg catctggagg ctggccacga taagctgccg ctggtggtgc cgatactgtt
     1021 ttatcagggc gaggccacac cttatccgct ctcaatgtgc tggtttgata tgttttactc
     1081 gccggagctg gcgcgacgcg tctataacag tcctttcccg ctggtggata tcaccatcac
     1141 accggatgac gaaatcatgc aacatcggcg gattgcgatt ctcgaactac tgcaaaaaca
     1201 tattcgccag cgcgacttaa tgttattgct ggagcaactg gtcacgctga ttgacgaagg
     1261 gtacactagc ggaagtcagt tagttgccat gcaaaactat atgctgcaac gcggtcatac
     1321 tgaacaagcg gatttgtttt atggtgtgct gagagacagg gaaacgggag ggaagtctat
     1381 gatgacgctg gcgcagtggt ttgaagagaa agggattgag aaggggattc agcagggaag
     1441 acaggaggaa aggcaagaat tcgccctgcg tcttctaagt aaaggaatgt ctcgggaaga
     1501 cgttgcagag atggcaaatt tacctcttgc tgagattgat aaggtaatta accttattta
     1561 agttacctgt gttatgacag atgacgtggg gtaaattaat aactggcgcc atcagccgta
     1621 gcgccagtta agtattacgc cagctcgacc attttgttgt cgatcaggcg agcatcgcca
     1681 agccaggcgg ctaccagaat tactgcccgt ttgctggttt cagaaacttc cagcaatgtg
     1741 tcggcatcgc gaatctgaat atcatcggcg cggaagcctt tttcattcag ttcttgcccc
     1801 gcaatggtaa taatttcatc gagatcccgt tccccagcct gcaatttgtc agcaatcgaa
     1861 cttaaaactt tgtacagacc aggcgcaatt ttgcgttgtt ccgccgtcag ataaccgtta
     1921 cgggaactta gcgccagacc gtctttggcg cgcataattg gcacaccgac aatctcaata
     1981 tcgaagccca tatcggcaac cattttgcgg atcaacgcca gttgctgaaa atctttttca
     2041 ccgaagcagg cgatgtccgg ctggaccagg ttgaacagct tgctgacaat agtcgaaacg
     2101 ccgcgaaaat gtcccggacg gctggcacct tccagcatgg tcgaaaggcc aggaacgtca
     2161 acgtaagtgt gggtttcagt accgttcggg tagatctctt ttaccgaagg ggcgaaaact
     2221 aaatccactt tacgtttgtt tagcttctcg cagtcctcct gcaaggtccg tggataacgt
     2281 gccagatctt ccgggcggtc gaactgcatc gggttaacga aaatactgac gacgaccaca
     2341 tcggcgcggg ctttggcttc gtcgaccagc ttcatatggc catcgtgcag attacccata
     2401 gtaggcacca gcgccacgcg cttgccttcc atacgcaggc ggcgaatttg ctgacgcagc
     2461 agcggcaggg tttcgataat taacacaacg tgactcctta atggaaactg tgttcttcgc
     2521 ccggataaac gccggactcc acttcagcca tatactgccg cacagccgcg cggatgtcgc
     2581 ccgtttcggc gaggaaattt ttagcgaatt taggaatgtg accgccggta ataccaaagg
     2641 cgtcgtgcat cacgaggatt tgcccgtcag tgacgttgcc tgcaccaata ccaataaccg
     2701 ggatcgccag tgcttcggta atacgttttg ccagctcaac cggcacgcat tccagcacca
     2761 gcagctgtgc ccccgcggct tctaaggcta atgcatcgct gagtagtcgc tcgctcgctt
     2821 cattgccgcg cccctgtact ttgtagccac cgaaaatatt cactgactgt ggggttaaac
     2881 ctaagtgacc acatacagga acggcacgtt cggtcagcat ttttacggtt tcgaccagcc
     2941 actcaccgcc ctcaattttg accatattgg caccggcacg cataaccgtt gcggcgtttt
     3001 caaaggcttg ttccggcgtg gcatacgcca taaacggcag gtcagccaat agcaggcagt
     3061 ttggtgcgcc gcgacgtacg gcggcagtgt ggtaggcgat atcagcaacg gttactggca
     3121 gagtggaatc gtggccctga acggtcatgc ccagcgagtc gcctaccagc atgacgttaa
     3181 gcccttcatc agcaaatagt ttcgcgaagc tgtagtcgta agcggtgatg gtcgcgaaac
     3241 gctttttatc ctgcttatat ttctgaagtg aagcaatggt ggtcggtttc ataacgtatc
     3301 ctgataaatg atgtcgtgtt gcctggcatt ttatcagtca cattggtggg ggcaatgatt
     3361 tatcaattac cgcaccgcca gatgatggcg gtgcggtgga cttattaagg gtaggttacc
     3421 tggaaagtac tggtggcttt aaactcacca gcttctattg gtatagttcc atcctgttgt
     3481 agcgtggcct ggaaatggag agggtatgtt atacctgtat cttgatccgg gtaaactcct
     3541 cctgttgtat cgtctttgcc tctcgggtcg taatctttat aaacagaatt tgaatcatta
     3601 ggtttcaatg tcattagagg atttttacta gttgctaaac cttctatgag tacaccgact
     3661 ccttttgctg cgtcattacc tgtgagagta ttaccaagga gttgcccgtt ttcagtacca
     3721 acct
//
LOCUS       DADLSB010000264         3632 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.265, whole genome shotgun sequence.
ACCESSION   DADLSB010000264 DADLSB010000000
VERSION     DADLSB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.265"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1393)
                     /gene="tssF"
                     /locus_tag="JN331_004087"
     CDS             complement(<1..1393)
                     /gene="tssF"
                     /locus_tag="JN331_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY7262040.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLT"
     gene            complement(1409..1903)
                     /gene="tssE"
                     /locus_tag="JN331_004088"
     CDS             complement(1409..1903)
                     /gene="tssE"
                     /locus_tag="JN331_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY7262041.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(1900..2730)
                     /locus_tag="JN331_004089"
     CDS             complement(1900..2730)
                     /locus_tag="JN331_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HAY7262042.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTALACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(2717..3631)
                     /gene="tagK"
                     /locus_tag="JN331_004090"
                     /pseudo
     CDS             complement(2717..3631)
                     /gene="tagK"
                     /locus_tag="JN331_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT          768 a          961 c         1075 g          828 t
ORIGIN      
        1 cggttagatc gtcccggcca ttgcgggcaa taagacaggg cagatcgcgg ttagtgacca
       61 tggccgtgat ggtgatgtgg cgcagatttt ccgggtacgg cgcttcatac tggtcaacca
      121 gcgacaggaa cacttccgag cccgtatagg gggtacgggt gccgtaacgg cgggcgtttt
      181 ctgataagcg gcggggctcc cggcgcaggg aaaaataacg tccgtgattg ccttcatcat
      241 tattgcgggt gtggtagagc gggcgaaagg ccatcttccg tgtggtgtcg gtttccagcc
      301 cttccacttc ttggaccgag aacacctcat aatccagcgg atgggttctg tccaccacca
      361 gatgctgttc cgtcgtgctg tgcgtgacat cgatccgggc ggtgacgcgg ggaaacaggt
      421 tgataaccgg ggtacagaac aggctgaact gtgccgcatt tgtctggtgg atcagccagt
      481 ccggcggcag gcggttcagc aggataacaa tctccgccac gccaccgtca attttctgta
      541 atccggcaga caggcctgtc ggggtgaaaa aatagaagcg ttccggacag gcaaaatatt
      601 catgcagcag gttatgaccg tgaaacacgt tccacgccag cggcagcagc ccctgatccg
      661 gctccaggcc ttcgtacata acgggctgtt gcagattcac atccagcgcg ccgtcaaaat
      721 gccccggaat gccagccagg gtcgcgatgg cgctggtatg tagaagttct aacaggtgtg
      781 aggccgtacg ctcctcgccg cagagataaa acggcagcct gtccagtccg gcaagctgac
      841 tgaacgttag ttcaccaaag gtgcgcaggg taatacgcag cgccccggcg acgtgaatgt
      901 tggccggcag gtagcggtgc agggcaggca tatccggtgg cgcggcggtc agtcgcgcct
      961 cttcgatggc gagcggccac agcgttacat cctggctgct gcgaaactgg caggcagtgc
     1021 tttcgccttc cggaatggcg gagaaaaagg ctgtgtcgcg cgggacggta aatcctttcg
     1081 ccagatcgcc ttcttccgta tcgggatgca actgcgccac gctcattgag ggcgtgggtg
     1141 tgacgtaatt ggggctgacc acttccagca ggcgctgggt gaaacggggg aattcagcgt
     1201 caatttttag ctgcgtgcgg gcggtcagga agctgaaggc ctcgaccatt cgctctacat
     1261 acggatcggc aatatccgtt ccctgcatgc ccagccgggc ggcgactttg ggatgacggg
     1321 cggcaaattc ggctcccgtt tcccgcagat agctcagttc gcggttgtag tattccagta
     1381 gccgtggatc catcggtctc tctctgaatt aaaaaaacgt aatgcgactc tgctccatat
     1441 ccagcgcgct acgtacccgg aatgcagtcg gatacggctg cgtgaggatc tcgccgcgaa
     1501 tttcaaactg taatgtgtta tggccactct ggcggcgctc atcctgaagt gggatgattt
     1561 gcagggtgga cgctttcagc cggggttcaa accgggtgat ggttcgcaaa atggccctgc
     1621 ggatatcgtc ccacttgtgc tcatacagaa agctgcccgc cagtggcggc aggccgtagt
     1681 taagtacgga cgctgccgcg tacggataac gtgcagcatc aatatcatct tcatggctga
     1741 cggtgttgag cagaaatgac aaatcccggc ggatgatctc cttaatctga accgggctga
     1801 cgctgatacc gtggtcgcgt ttctggtttg gggcgtcgtc acacaggcgg tcaaacagcg
     1861 tgggcagcag gtgattgcct ggacgctggc gggaagcgct cacgcgtcgc ttccttcagc
     1921 ggtgttaaag gtacaggtgg acaaatccag caggctgata tcgccgtgac cggtgagcca
     1981 caccctctga cccagcgcct gtaccagtgt ctctcccgga ccgtcctgcc agaccgtttc
     2041 ccggcacaga cgcagggagt cggcagcctt ttcagagccg ctgtagcggg gaaacagcca
     2101 ggcactgtgt gtatcgccat tgttcagggt gacgttgacg ggtttccaga tcaaatcgat
     2161 cagatgcgca ggccgcggcg atttcagcga acagatttgt gaaaacggca gccagatata
     2221 tgctccgccc gcgatcagtt cgagtaccgg accggtacgg gagtcgctgt cgctaatcca
     2281 gtcaaatgcg ccgccattcc actgcccgga agtgtctgtt atggcctcaa gcgcgcggct
     2341 gcggtgagca tcagcttcct gcgcttcacc acgggcgtta caggcgaggg cggtcagcag
     2401 atcctccatc caggcgggcg gcgggagaat aacaccgggc cgctgttcgc cctgaaagca
     2461 ggcgtggcgg tatatctcac agcggatcag ctcgccaaat acctgcgctt cgcgagtata
     2521 atttgcgtcc atccgggcgc atagctggat ctgttgcagt gcccgcgacc agtcgcctgc
     2581 cacgcacaat aactgaaaca ggctgtggcg gcgggaggcc tgcgctgggt gcatcctgac
     2641 ctgttgctct gccatctgaa tgccctccac cacggaataa tcccgcagca gcgccgacaa
     2701 ggtaatcggt agctcgttat tgtttgtcat gatatatctc tccgtgttgt gccgggttta
     2761 tccggtaatg gctgtcaatg ccaatgatgc gatgctcttt ccgtgtcaga tctggcagta
     2821 cggtgttatg cgctgtttcc ggggcgtatt ccggggaaag taaatgcagg atatcagggg
     2881 gagtcttttc gacaggccag atagtttcat cttcgccagt ggcatccagt acattgagaa
     2941 tagcgtcaat gcttaaccca ccagtgacca tatcctgcaa tgtctcggct tcggtgacta
     3001 ggtcacgcaa tgaatccaga tcctgcgagg agggctcttc gttaaaagga aaaggatcag
     3061 catggcgatt aagatcttgt ccgggagata acaacgcctc gcggtattcc tgataaagtc
     3121 gggtcagggc atttcctttt tccgcacctt ctccggtgga ggaggctcca tgaacaggaa
     3181 tgatatcgaa tggattttgt gatgtcagat gctgctgcct gaaccaatcc agatccagat
     3241 attcggttat tgcttccgac gtgatggcag tctgcgatat cgtttttact gtgctcaggt
     3301 ccggagatga tggattgcca tgtacaaggc actatgaaga taacccccat tctatcaggt
     3361 cgccttcatt cagacgcata cggtgatgag gttccacaat ctggtcgttt accgtacagc
     3421 aatattcggt agactgattg ataatccacc atgcttcttc gtggcgggtc agagtgaggg
     3481 caatctttac atcatcattc accttaaaaa caggaatcca gggagaggta acggtgaaga
     3541 taatttgtgt tccttcagtc gcgtaaattg tgttatcggc gatcccgtgc gcctgtattt
     3601 tttggagtcc ccacgtaata tttggtttca tt
//
LOCUS       DADLSB010000265         3625 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.266, whole genome shotgun sequence.
ACCESSION   DADLSB010000265 DADLSB010000000
VERSION     DADLSB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.266"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            77..904
                     /gene="nadE"
                     /locus_tag="JN331_004091"
     CDS             77..904
                     /gene="nadE"
                     /locus_tag="JN331_004091"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY7262043.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVAYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            1134..2021
                     /gene="cho"
                     /locus_tag="JN331_004092"
     CDS             1134..2021
                     /gene="cho"
                     /locus_tag="JN331_004092"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY7262044.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(1981..2556)
                     /gene="ves"
                     /locus_tag="JN331_004093"
     CDS             complement(1981..2556)
                     /gene="ves"
                     /locus_tag="JN331_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY7262045.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGELSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(2759..3244)
                     /gene="spy"
                     /locus_tag="JN331_004094"
     CDS             complement(2759..3244)
                     /gene="spy"
                     /locus_tag="JN331_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY7262046.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEECRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT          920 a          858 c          897 g          950 t
ORIGIN      
        1 cccgctgtcg cttgtcgttt cagtagcaac gggttagctt taaggaagtt ttgtcttttc
       61 tgtctggagg ggttcaatga cattgcaaca acaaataata aaggcgctgg gcgcaaaacc
      121 gcagattaat gctgaagagg aaattcgtcg tagtgtcgat tttctgaaaa gctacctgca
      181 aacttatccg ttcattaaat cactggtgct cgggatcagc ggcggtcagg actccacgct
      241 tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc caggaaactg gcaacgaatc
      301 actgcaattt attgccgtac gcctgcccta tggtgttcag gctgacgaac aagattgcca
      361 ggatgccatt gcctttattc aaccggatcg cgtattaacc gttaatatca agggcgcggt
      421 attggctagc gagcaggcat tgcgggaagc aggcattgaa ctgagcgatt ttgtccgtgg
      481 caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc attgcgggta tgaccagcgg
      541 tgtcgtggtg ggcaccgatc atgcagcaga agccattacc ggattcttca ctaaatatgg
      601 tgacggcggt acggatatta atccgctgta tcgtctcaac aaacgccagg gtaaacagtt
      661 actgacggca ttaggttgcc cggaacacct ttataagaaa gccccaactg ccgatctgga
      721 agatgatcgc ccttctctgc cagatgaagt ggcactcggc gtggcctatg acaatatcga
      781 cgactatctg gaagggaaaa acctacctga acaggtcgcc agaacaatag agaactggta
      841 tctgaaaacc gaacataaac gccgtccgcc aattaacgtt ttcgatgatt tctggaaaaa
      901 gtaataattt gcacctattg gattgtgcga aaaagagtaa tttgttcacg ccggatgcgg
      961 cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca atacattgca ggagtcgaat
     1021 aggcctgaca ggcgtagcac gtcagacggt gtaacctttg tcatcaaccc gcctcttttt
     1081 taatcgcctc ccgcctgtta cactggatag ataaccagca ttcggagtca acagtggtac
     1141 ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc aatttatgaa tatcccgaac
     1201 atttacattc attccttaat gacttaccca cccgaccagg ggtgtatctg tttcatggcg
     1261 aaagtgacac catgccgctc tatatcggca aaagcgttaa tatccgcagt cgggtgcttt
     1321 ctcatttacg caccccggat gaagccgcca tgctacggca atcccggcga attagctgga
     1381 tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc gcgattaatc aaagaacaac
     1441 agccgctgtt taataaacgg ttgcgccgca atcgccagct ctgtgccctg caattaaatg
     1501 aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt ttcacgagcc cccaacctgt
     1561 ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt gcagagcatc gctgatgaac
     1621 aaaaactttg ttatggcctg ttgggactgg aaccgttaag tcgcggtcgt gcatgttttc
     1681 gttcagcgct aaaacgttgc gccggagcat gctgcggtaa agagagccat gaggaacatg
     1741 cgctacgctt gcgccagtct ctggagcgtt tgcgggtggt gtgttggcct tggcaaggtg
     1801 cggtggcact gaaagaacag cacccggaaa tgactcaata tcatattatt caaaactggc
     1861 tgtggctggg ggcggttaat tcgctggaag aagcgacaac gttaattcgg acacccgccg
     1921 ggtttgatca cgacggttat aaaattcttt gtaagccgct gctttccggt aactatgaaa
     1981 ttactgaact tgatccggcg aatgaccagc gagccagttg atttcactga acaacagttt
     2041 accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg aaccagcacg cgccctggtc
     2101 ggtagtgagc agtttgtcgc ccagttgcca cgccccgtta atcacaaaca ccaccccgcc
     2161 gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg actttcgcct tacagacatc
     2221 caggcgcgtc ataatattga aatccatcga catctgccct gccgtcagtt tcgcttttac
     2281 cacctggtcc gccgtaaagg caaaaggctg taacggtttt aaggtatggt taaagcgatc
     2341 tgcgctttca aggagcatct cgccgccttc cagcaacgtc actatccttt ccatgccggg
     2401 aaacaaagaa agctccccat tcgccgcgat ggatgcaatg ctggcacgcc agtaaaaatc
     2461 acgttttgcc ggagggaaag tgcaaatttc gcgcgtttca ccggcagcgt ttcgccacag
     2521 attcaccgac attttacgca tatcaaagta ttccatactc gctcccggcc cgttcttttc
     2581 attgttatcg ttcaggtaac cgtggatggt tatcggcaac tcgggtgatt tacgttagtg
     2641 gtgatcaggc tttttttaca actgtttgag aagagaaaag aaaaccgccg atcctgtcca
     2701 ccgcattact gcaaggtagt ggacaagacc ggcggtctta agttttttgg ctgaaagatt
     2761 attcagcagt tgcaggcatt ttaccttttg ccgctggacg ttctgtcaga cgcttctcaa
     2821 aattagcatt aaattgcttt ttctgttccg gcgtcagaat gttgtaaatt ttgttctggg
     2881 tttccatgtg cgccagcatg ttagctttgc gctgttcttc catttttgcg atctgcgctt
     2941 cagcttttgc tttatcgaag gtatcgctgg caatgatgtc atgcattgcg cggcattctt
     3001 ccagcggcgg acgtttcatc tggtcacgct ggcctttcat gatttcgtgg atctgctgtt
     3061 tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc ctgatgcgga ccgaacttgc
     3121 ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc ggtagtggtg tctgcggcat
     3181 gggccaagtt agccgcgcca agagccaggg tagaggcaac aaacagtgca gttaatttac
     3241 gcatattcta tatccttcct ttcagttatt tattacggct ttcttaagta gcgtgccgtg
     3301 ttgacgagat taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg
     3361 aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt
     3421 gatgagagaa tattacaaca cgatgatttt gcagagatta tgaagaacta taccggatga
     3481 ctggtgataa ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact
     3541 taatacccgc agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc
     3601 cgcgcgtaat cccaacgcta ccaac
//
LOCUS       DADLSB010000266         3610 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.267, whole genome shotgun sequence.
ACCESSION   DADLSB010000266 DADLSB010000000
VERSION     DADLSB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.267"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..796
                     /locus_tag="JN331_004095"
     CDS             <1..796
                     /locus_tag="JN331_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7262047.1"
                     /translation="WLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            807..1784
                     /locus_tag="JN331_004096"
     CDS             807..1784
                     /locus_tag="JN331_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7262048.1"
                     /translation="MKRAPLITGLLLISTSCAYASTLSSTNWQYACSCSAGKAVKLVY
                     MVSPVLTTTGHQTGYYKLNDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYS
                     AATQTGVCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGS
                     PSTGDFHDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVD
                     FDITYDCGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPNVGIRIENLGGGVA
                     NIPFQNGILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1827..2687)
                     /gene="rsmI"
                     /locus_tag="JN331_004097"
     CDS             complement(1827..2687)
                     /gene="rsmI"
                     /locus_tag="JN331_004097"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY7262049.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGALLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2752..>3610
                     /locus_tag="JN331_004098"
     CDS             2752..>3610
                     /locus_tag="JN331_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7262050.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNI"
BASE COUNT          922 a          935 c          883 g          870 t
ORIGIN      
        1 ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc gcccaaccca
       61 acatgaaatg cgtcttgatg gctcactgct ggatgatggt cgcctgagct atagcctgga
      121 acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt accgttcacc
      181 ttatggaacc ttcagtgccg gatacagtta cggtaatgac agcagccaat acaattacgg
      241 cgttaccggc ggcgtggtta tccatcctca tggcgtgacg ctctcgcaat atctgggcaa
      301 cgcttttgcg cttatcgatg ctaacggggc ttctggcgtg aggatacaaa actatccggg
      361 gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt atcaggaaaa
      421 ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgaac aaacaacaca
      481 gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca atatcggtta
      541 tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg ttgccctttg gcgctcttgc
      601 cagcaacgat gatacggggc aacaaagtat cgtcgatgag ggcggcatac tatatctctc
      661 tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg cagatcaaca
      721 atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat tacaaggcac
      781 agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca ggacttttgt
      841 tgatatccac atcctgcgct tatgcctcaa cgctaagtag cactaactgg caatacgcct
      901 gttcctgctc tgcgggtaag gcagttaaac ttgtctatat ggtcagcccc gtacttacca
      961 ccactggaca tcagacagga tattacaaac tcaatgacag cctggatatt aaaaccacat
     1021 tacaggcaaa cgacattcca ggactcacaa ccgaccaggt tgtctctgtt aacacccgat
     1081 tcacacagat aaaaagcagc accgtatatt ctgctgcaac ccaaacgggt gtttgccagg
     1141 gtgacacgtc tcgttatgga cccgttaata ttggtgcaaa taccaccttt accctgtatg
     1201 tcaccaagcc atttctcggc tcgatgacca ttccgaaaac ggatattgcc gtcattaaag
     1261 gcgcgtgggt cgatggaatg ggaagcccgt ctacaggtga cttccatgat ttagtcaagt
     1321 tatcgattca gggaaatctc accgccccac agtcgtgcaa aattaatcag ggcgatgtta
     1381 ttaaagttaa ttttggattc atcaatggtc agaagtttac cacccgcaat gccatgccag
     1441 acggttttac tccagtagac tttgatatca cttatgactg tggtgatact tcaaagatta
     1501 aaaactcgtt gcaaatgcgc attgacggta caactggggt agtagaccag tacaacctgg
     1561 tcgccaggcg aagaagttca gacaatgtgc ccaatgtcgg tattcgtatt gaaaatctcg
     1621 gcggcggagt tgcaaatatt ccttttcaga acggtatcct tcccgttgat ccttccgggc
     1681 atggcaccgt caatatgcgc gcctggccag ttaatctggt cggtggtgag ctggaaacag
     1741 gaaaatttca gggcacagcc accattaccg tcatggtgcg gtaaaagaag acgttgcgtt
     1801 ttgattaacg caacgcttcg gcactgttac ccctgctgct ccagcgcata cttatacagc
     1861 gcatttttct tcacgccatg aatttctgcg gccagcgccg ccgctttttt cagcggcagt
     1921 tctgcctgta gcagcgccag cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct
     1981 ttatgacctt cgacaatcag caccatctcg cctttgcgac ggttttcatc ttcctttacc
     2041 cacgccagca gcgcgccaac gggcgcgccg tgaatggttt cccaggtttt ggtcagctca
     2101 cgcgccagaa ccacgtagcg ggattcgcct aataccgcaa cgatatcttc caggctatct
     2161 aacagacggt gggtagattc ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt
     2221 agcgcatcac ggcggccttt tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca
     2281 gatggtaaac ccgcggcgct taacgcagtg atagcagcac acggtccggg tagcggcacc
     2341 acacgaatcc ccgcttcacg gcaggtacgc accagatggt agccaggatc gttaattagc
     2401 ggcgttccgg catcggaaac cagcgcaatg ttttgcccct cttgcagctt cgccagcagc
     2461 gtttcggctt tttgttgttc gttatggtcg tgcagcgcaa acaaccgggc attaatccca
     2521 aaatgttgca gcaataaacc ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc
     2581 tgtaatacct ctaacgtacg ctgggtgata tccgccagat tgccgattgg cgtcggtaca
     2641 atgtaaagct ggccctgaga attatccgcc gattggtgtt gtttcattgt gtcgtccgta
     2701 ttgccgattt aatattgagc attgcgtaaa aaaaatatca ctggatacat tatggtaccc
     2761 tcaacatttt ctcgtttgaa agccgcgcgt tgtctgcctg ttgttctggc agccctgatt
     2821 ttcgccggtt gtggcaccca tactcccgat cagtccactg cttatatgca gggcacggcg
     2881 caggctgatt ctgcctttta tcttcagcag atgcagcaaa gctctgatga taccaggatc
     2941 aactggcaat tactcgccat tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt
     3001 gagttgttta accaactacc gcaagaactg aacgattctc agcgtcgcga gaaaacactg
     3061 ctggcggcag agattaaact ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg
     3121 aaaatcacac ctgccgattt agaacaaaac cagcaagcgc gttactggca ggcaaaaatc
     3181 gatgccagcc aggggcgtcc ttccattgat ttactgcgcg cgttaattgc tcaggaaccg
     3241 ctgcttggcg cgaaagaaaa acagcagaat attgatgcca cctggcaggc gctctcctcc
     3301 atgactcagg aacaggcgaa tacgctggtg atcaacgccg acgaaaatat tctgcaaggc
     3361 tggctggatc tgcagcgcgt ctggtttgat aaccgtaacg atcccgacat gatgaaagcc
     3421 gggatcgccg actggcagaa acgttatccg aacaatccgg gcgcgaaaat gctgccaacg
     3481 cagttggtta acgtaaaagc gtttaaacca gcctcgacca acaaaatcgc cctgctgttg
     3541 ccgctgaatg gccaggcagc ggtatttggt cgcactattc agcaaggctt tgaagccgcg
     3601 aaaaatatcg
//
LOCUS       DADLSB010000267         3588 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.268, whole genome shotgun sequence.
ACCESSION   DADLSB010000267 DADLSB010000000
VERSION     DADLSB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.268"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..372
                     /locus_tag="JN331_004099"
                     /pseudo
     CDS             1..372
                     /locus_tag="JN331_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427357.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            381..809
                     /locus_tag="JN331_004100"
                     /pseudo
     CDS             381..809
                     /locus_tag="JN331_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
     gene            870..3293
                     /locus_tag="JN331_004101"
     CDS             870..3293
                     /locus_tag="JN331_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HAY7262051.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKTVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAI
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTKMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPIPAYTSGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            3304..>3588
                     /locus_tag="JN331_004102"
     CDS             3304..>3588
                     /locus_tag="JN331_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HAY7262052.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVD"
BASE COUNT          984 a          903 c          898 g          803 t
ORIGIN      
        1 tcattttcct ccagccagtc accagcgaaa aattcgaacg taaaccgatt tactggatcc
       61 tgagtgaagt cgcgaaacgt cttggacctg acgtctatca aaagttcaca gaaggtcgca
      121 cgcaggaaca atggttacaa catctgtacg ccaaaatgct tgccaaagat ccggcgttac
      181 cgtcttacga cgaactgaaa aaaatgggta tctataagcg taaagatccc aatggccatt
      241 ttgtcgccta caaagcattt cgtgacgacc ccgaggcaaa tccacttaaa acgccttccg
      301 gtaagattga aatttattcc agcaagctgg cggaaattgc ccgtacctgg gaactggaaa
      361 aagatgaagt gataagccca ctgccagttt atgcctcaac ctttgaaggc tgggactccc
      421 ctgaacgtag aaccttccca ctgcaactgt ttggtttcca ttacaaatcc cgtactcact
      481 cgacctacgg caacattgat gtcctgaagg cagcctgccg tcaggaggtg tggatcaacc
      541 ctatagatgc acagaaacgt gggattgcca acggcgatat ggtgcggttg tttaaccatc
      601 gtggcgaagt tcggctacca gccaaagtaa caccacgcat tctccctgga gttagcgcta
      661 tgggccaggg agcctagcac gagcccaata tgtctggcga taaaatcgac catggcggat
      721 gtgtgaatac gctaaccact ctgcgccctt caccactggc gaagggaaac ccgcagcaca
      781 ctaatctggt cgagatcgaa aaaatataac ccactacaac cataaattct ggcatgacat
      841 tttgtttgaa aagcaataag tgagtaatga tgaaaatcca taccacagag gcgctcatga
      901 aggctgaaat cagccgtaga agtctgatga aaacctccgc acttggcagt ctcgcgctgg
      961 caagcagtgc tttcactctg ccattttccc aaatggtccg ggcggcagag gctccggtag
     1021 aagagaaaac ggtctggagt tcctgcaccg ttaactgcgg gagccgctgt ctgctacgtt
     1081 tgcatgtgaa agatgacacc gtgtactggg tggagtctga tacgacaggt gacgacgttt
     1141 acggtaatca tcaggttcgg gcatgtttac gcgggcgctc tatccgccga cggatgaatc
     1201 atcctgacag gttgaaatat cccatgaagc gcgtcggcaa gcgcggtgaa ggtaaatttg
     1261 aacggataag ttgggacgaa gccctagata ccatcagtga taatcttcgg cggatcctga
     1321 aagattacgg caatgaggct gtacatgtcc tgtacggaac aggcgtagat ggcggaaata
     1381 tcaccaactc gaacgtcccg taccgtctga tgaactcttg cgggggtttt ctcagtcgct
     1441 atggcagcta cagtaccgca cagatcagtg ccgcaatgag ttatatgttc ggtgccaatg
     1501 atggcaacag cccggatgat atcgccaata cgaaactggt cgttatgttc ggaaataacc
     1561 cggcagaaac gcggatgagc ggcggtggtg tcacttacta cgtcgagcaa gcccgcgaac
     1621 gttcaaacgc acgcatgatc gtcatcgatc cacgctataa cgacacagct gccgggcgtg
     1681 aagatgaatg gctgcccatt cgccctggca ccgatggcgc actggcctgt gcgattgcct
     1741 gggtactgat tactgaaaac atggtcgatc agccatttct cgacaaatat tgtgttggtt
     1801 acgatgaaaa aacgctgccc gccaacgcac cacgtaacgc gcattataaa gcctatattc
     1861 tgggcgaagg gcctgacggc atagctaaaa cgccggaatg ggcagcaaaa atcaccagca
     1921 tcccggcaga aaaaattatc cagttggcac gagagatcgg ttcagcaaaa cctgcttata
     1981 tttgtcaggg ttgggggcca caacgacact ccaacggcga acaaacgtcc cgcgctattg
     2041 ccatactttc cgttcttacc ggtaacgtcg gcataaacgg cggcaactca ggtgtacgcg
     2101 aaggtagctg ggatctgggg gtagaatggt tcccgatgct cgagaatcct gttaaaacgc
     2161 agatttccgt ttttacatgg acagatgcca tcgaccatgg tacgaaaatg accgcgaccc
     2221 gtgatggtgt tcgtggaaaa gaaaaactgg atgtccccat caagttttta tggtgctacg
     2281 ccagtaacac attgatcaat caacatggcg acatcaatca cacccatgag gtgcttcagg
     2341 atgacagcaa gtgcgagatg attgttggca ttgaccactt catgacggcc tcggctaagt
     2401 attgcgatat cctgttgcct gacctgatgc caacagagca agaagacctt atctctcatg
     2461 aatctgcagg gaatatgggc tatgtgatcc tcgcccaacc cgcaacctca gcaaaatttg
     2521 aacgcaaacc catctactgg atgctgagtg aagtcgccaa acgcttagga ccagacgttt
     2581 atcaaacctt tactgaaggt cgcagtcagc atgaatggat caaatatctc catgcgaaaa
     2641 cgaaggaacg taaccctgag atgcccgact acgaggagat gaaaacgacc gggatcttta
     2701 agaaaaaatg cccggaagaa cactacgtcg ctttccgcgc attccgtgaa gatccacagg
     2761 caaacccgtt gaaaacacct tcggggaaaa tcgaaattta ttctgaacga ctggcgaaga
     2821 ttgcagatac ctgggaattg aaaaaagatg aaattattca tcccattcct gcgtatacct
     2881 caggttttga tggctgggac gatcccctgc ggaaaaccta tccactgcag ttaacgggct
     2941 tccactacaa agcgcgtacc cactccagct acggcaatat tgatgtgtta cagcaggcct
     3001 gcccacaaga ggtgtggatc aaccccattg atgctcaggc acgcggtatc cgtcatggcg
     3061 ataccgtgcg ggtatttaac aataatggag aaatgctgat tgccgcaaaa gtgactccgc
     3121 gtattctgcc tggcgttacc gccatcgggc aaggtgcgtg gcttaaagcg gatatgtttg
     3181 gtgaccgggt cgatcacggc ggcagtatca atattctgac ctctcaccgc ccttcaccgc
     3241 tggcaaaggg aaacccgtcg cacagcaatc ttgtccagat cgaaaaggtt taaggagtag
     3301 cccatgacca cacaatatgg attttttatc gattccagcc gctgtactgg ctgtaaaacc
     3361 tgcgaactgg cgtgcaaaga ttttaaagat cttggcccgg aagtcagttt ccgccgtatt
     3421 tatgaatatg ctggcggcga ctggcaggaa gataatggca tctggcacca gaacgtgttc
     3481 gcttattatc tctccatctc ctgtaaccat tgcgatgacc ccgcatgtac aaaagtctgc
     3541 cccagcggcg caatgcataa gcgtgaagat ggctttgtgg tggttgat
//
LOCUS       DADLSB010000268         3543 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.269, whole genome shotgun sequence.
ACCESSION   DADLSB010000268 DADLSB010000000
VERSION     DADLSB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.269"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..702)
                     /gene="tqsA"
                     /locus_tag="JN331_004103"
     CDS             complement(<1..702)
                     /gene="tqsA"
                     /locus_tag="JN331_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY7262053.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALN"
     gene            1114..1479
                     /gene="mdtJ"
                     /locus_tag="JN331_004104"
     CDS             1114..1479
                     /gene="mdtJ"
                     /locus_tag="JN331_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY7262054.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            1466..1795
                     /gene="mdtI"
                     /locus_tag="JN331_004105"
     CDS             1466..1795
                     /gene="mdtI"
                     /locus_tag="JN331_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY7262055.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1834..2655)
                     /locus_tag="JN331_004106"
     CDS             complement(1834..2655)
                     /locus_tag="JN331_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY7262056.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(2755..2838)
                     /locus_tag="JN331_004107"
     CDS             complement(2755..2838)
                     /locus_tag="JN331_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262057.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(2931..3266)
                     /locus_tag="JN331_004108"
     CDS             complement(2931..3266)
                     /locus_tag="JN331_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY7262058.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
BASE COUNT          904 a          831 c          857 g          951 t
ORIGIN      
        1 attaagcgca aaggccagca atccccagac aaaagcgaag cgaacatcga gtgcggcgag
       61 catcgcccag gcgaccaggc cggtgatgat gctgatggct gttttcagca ccagataatg
      121 agaaacactg tcaatcgcac gttgaatcgc cgccatccct tcaaccggac gcgccatcat
      181 ttgctgaaat tttccgggca attgtggcac ttcgagcagc ataaacagca ccgtcagcag
      241 caataaaaat attgatgaca tggcattaga taactgcgtc aataagttgg tgagcaacgt
      301 catcgccgcg ttcggatcaa tgtaatgcgc cagttggtca actgagacgt caatccctac
      361 gcgttgcaac aacggctcaa gagcttgcag cggcgtcata atagagttgc gatattgcgg
      421 taacgtccgc gtcaactcgt tgagcgcgga acccagataa gctaatagca acaccatcgc
      481 catcacgatg attgtcatca aaatcgacac tgccagtaca cgcggcacac gccagcggac
      541 catatgttgc accagcgggt taagaataac agcaataaat aatgcgagaa taaacggcac
      601 gatgatctcg gcggcaaaac ggataccgca gagaataatg accagcattc ccaacataat
      661 gacgattttt aggccattga gcgtgatgat cggctttgcc atgcttgtcc ccgttttttc
      721 ttcctttgct aataataatg tctttttcgc tccgtcatca taacgaaaat caaaccgctc
      781 aggtaaagaa gtgaaaatat tttgagttaa ttcttaagct atgatacaaa tcaggcgtgt
      841 tcaactaccg aggacaatta tcatctgcga tgacgagaag caacactgcg gataattgta
      901 atattatgga caatttgttc aggacgttat tttctacatt tacccatctt cgtacctcat
      961 ccactatctt gctcgtaggt gagcagcact ggcgcaacgc gctttagtcc tttcttctgc
     1021 ctaagcttgc cctatgcgca gggcttgacc actttacttt ggtttcgctg aattaagcga
     1081 aaattaaaat agttctcttg caggagaagg acaatgtata tttattggat tttattaggt
     1141 ctggctattg ctacagaaat taccggtacg ctgtcaatga aatgggcgag cgtcagtgag
     1201 ggaaatggcg gctttatttt aatgctggtg atgatttctc tgtcgtatat atttctctct
     1261 ttcgccgtta aaaaaatcgc cttaggcgta gcttatgcgc tgtgggaagg tatcggtatt
     1321 ttatttatta ccttgtttag cgttttgtta ttcgacgaaa gtttatcgct gatgaaaatt
     1381 gccgggttaa ccaccctggt cgccgggata gtgttgatca aatcaggtac ccgtaaagcg
     1441 cgtaaacctg aactggaggt gaaccatggc gcagtttgaa tgggttcacg ccgcctggct
     1501 ggcattggca atcgtgctgg aaatcgttgc taacgtcttt ttgaaatttt ctgacggctt
     1561 tcgtcgcaaa atatttggct tgctctccct ggcggcggtg ctggcggcct ttagtgcgct
     1621 ttctcaggcc gttaaaggga tcgacttgtc tgtcgcttat gcattgtggg gcgggtttgg
     1681 tattgccgcc acgttagccg caggttggat cttgtttggt caacggttaa atcgtaaagg
     1741 ctggattggc ctggtcttgc tgttggctgg aatgatcatg gtgaaacttg cctgatgaag
     1801 acgctgcccg cgctgtcggg cagcgtttga acattatttt tgcgacagtt gatccagctt
     1861 gtcgcgaaaa ccggtaacag aaatggcccg gttatcggcg cgccagcgat ctttcgcggc
     1921 aggagccgaa ctttgcaccc caattaattg ccagccgtca tcggtatgca acatcagagg
     1981 cgaaccgctg tcacccggca aggtatcgca ctgatgtgac atcaccgacg tttgcgccca
     2041 gccagtcact tcacagtttt gatgactgta taacgtatcg agatgatctt cagggtatcc
     2101 cgcctgagtc actttacgac cagccgattt taatgcggca gtaagcgcgg ctttatctcc
     2161 ctcaaataac ggcaacggtg taatgccaga agggggatta cgtagcacaa tcaatccgaa
     2221 gtcccacggt gcagctgcgg gaggaacaat ccaaccatcc ccatctgctt ttaaccgctt
     2281 tcccagtgtc ggatcaacgc ggccttctat gtcgtggatc tcatagcgcc aaagaccttt
     2341 atttgacaca aaacgcagcg ctaccgcctt atccgcttta cccttaggcg gcgtcaataa
     2401 acagtgtcct gccgttaatg ccagattggg tgcaatcagc gtcgccgtac ataaattgcc
     2461 gctggccgtt tccagttgcc caaccgcatc ccacggtgat tgggtcgtgt cattcactgg
     2521 cacacgatca tcatgaccaa aaaacagggt gctgacctca tcgtttgccg atttggcaac
     2581 gtctggttta tctgcaaaca caaaagcaga tgtcaaacta attgcaccca acactacagc
     2641 aatggttgta cgcatatcac actctggtgg gtaattatgc ttattaaaag caaaccctca
     2701 taaatactat agacgggacg ggatgaaagt gggagtaaaa tcagataact acagtcagga
     2761 aagataaaaa cgggcggcaa tcagtgcgca taaaataagg atgataagaa tgaactcaaa
     2821 gcgataacgg cccaccatac taccctccgc taaaggcggt gctaaacgtt acccgcttag
     2881 caccgtaatt ttcaggcgcg agggggcgcg ccagcattac tgttgaaaac ttacgctgcg
     2941 ggttgtgcag caggttttgc cggttgctga tggccgtgtt tcccggcgtg tttcttcgct
     3001 gcctgcgctt tttgttcagg ggctttctgt tcagctttcg tatttttatg atgcttttta
     3061 gccgcctgcg ctttctgggc aggtgctgct ttatgctgtt ttttatgatg tgtagttttc
     3121 gctggcgctg ctttggtggt cgtcgcagtc ggagcaggtg tggtcgcagt ctctgcagca
     3181 aaggcggcag aagacagacc catagcagcg gcaacaacca gagctaatac ttttttcatt
     3241 gtcataccct caatttgttt tttcatttaa ccccactgcg gggccgttga aataactata
     3301 tccctgggaa tggcagactt ccgtgagtgg ttggtttcag cgtgtaacga tatgtacaaa
     3361 cgctgaataa attacagcgt tgataataac gtattgtgac ttaaggaaaa tttagctacc
     3421 aataatagta gtcttgatcg ggttaactat tatactactt acaaagcggt agaataactg
     3481 cgcatcagta ataaatgaca cacagcaaaa tgaatccgtt tatttgggta cttataatcc
     3541 tga
//
LOCUS       DADLSB010000269         3543 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.270, whole genome shotgun sequence.
ACCESSION   DADLSB010000269 DADLSB010000000
VERSION     DADLSB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.270"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1031)
                     /gene="curA"
                     /locus_tag="JN331_004109"
     CDS             complement(<1..1031)
                     /gene="curA"
                     /locus_tag="JN331_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY7262059.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLAPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGEMLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMVQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAG"
     gene            1212..1730
                     /gene="mddA"
                     /locus_tag="JN331_004110"
     CDS             1212..1730
                     /gene="mddA"
                     /locus_tag="JN331_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY7262060.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1727..2176
                     /locus_tag="JN331_004111"
     CDS             1727..2176
                     /locus_tag="JN331_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7262061.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2177..2410)
                     /locus_tag="JN331_004112"
     CDS             complement(2177..2410)
                     /locus_tag="JN331_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAY7262062.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2496..2669)
                     /locus_tag="JN331_004113"
     CDS             complement(2496..2669)
                     /locus_tag="JN331_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY7262063.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2864..2959
                     /gene="yncL"
                     /locus_tag="JN331_004114"
     CDS             2864..2959
                     /gene="yncL"
                     /locus_tag="JN331_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY7262064.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(3361..>3543)
                     /locus_tag="JN331_004115"
     CDS             complement(3361..>3543)
                     /locus_tag="JN331_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HAY7262065.1"
                     /translation="VIDELLRIKWWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQ
                     ERDYKKFNLSEVFRGL"
BASE COUNT          875 a          887 c          803 g          978 t
ORIGIN      
        1 tcacccgcga cgcggatcac cactttgccg aagtttttac ccttcagcag gccgataaac
       61 gtctgtggcg cattctctaa accatcagta atatcttcgc ggtagtggat tttatcctct
      121 ttcacccatt gcaccatctc cttctgaaac tcatggatgc ggtgaccata atcctgagcg
      181 ataataaacc cttgcaagcg aatacgtttt ttcagcaccg ttgccatcaa caggggtaaa
      241 cgatccggac cgggtggtag ctctgtagcg ttatagctgc tcactaagcc acaaacggga
      301 atgcgcgcag atgtattaag taatggtagc actgcatcaa ataccttacc gcccacgttt
      361 tcataataga tatcaatacc ttttgggcac gctttcgcca gttgttcggc aaaatcgtcc
      421 gcgtggtgat caaggcaaac atcgaagcct aacacctcgg tagcatggcg gcatttttcc
      481 gcgccaccgg ctacccccac cactctgcaa cctttaagtt tgccgatttg ccccaccgtc
      541 gcccccactg gtcctgtcgc cgcagctacc accaacattt cgccctcttt aggctgaccg
      601 atatccagca gccccatata agcggtaaag cctggcatcc ccagcacacc cagcgaccac
      661 gatggatttt gcggatgatc gccaagtttc accaggtcat caccactgga tatgtcatag
      721 tcctgccatc cactgtagcc cagcacccag tcgccaggct gataatcagg atgattcgac
      781 tccacgacac ggctcaccgt accgccgacc atcacgccgc caatatcaac cggcggtgaa
      841 taagatggct catcgctcat acgtccacgc atatacgggg ccagggacaa ataaactgtg
      901 cgcagtaaca cctgtccttc acccggtgtg gcgacatcat cttcttcaag acggaaattc
      961 tccggaaccg gtgcgccatg tggacgcgag gccagaaccc aacgtcgatt acgctgcttt
     1021 tgttgcccca taatgctctc ctgtgctttt ggcatgaatc catcaatagt agcaggctca
     1081 cgggattggt tttgtttgca gccgtcacat cccctctgca acacgcaata atttcatcga
     1141 ggaatccgtt gcttcgggct gttttcgata gtattgataa catcatcatc attattttat
     1201 tgaggccaga catgtccatc cgttttgccc gcaaagccga ctgtgctgcc attgcggaaa
     1261 tttataacca cgccgtgttg tatacggcgg ctatctggaa tgaccaaacg gtggatgcag
     1321 ataaccgcat tgcctggttt gaagcgcgga ccatagcagg ttatccggtg ctggtaagtg
     1381 aagaagatgg cgtagttacg ggatatgcct cgtttggcga ctggcgtagt ttcgatggtt
     1441 ttcgccatac cgtggaacat tcggtttatg tccatcccga tcatcagggc aaaggtctgg
     1501 ggcgtaaatt gttaagccga ttgattgatg aagcgcggga ttgcgggaag catgtcatgg
     1561 tcgccgggat cgaatcgcaa aatcaggctt cgctgcatct gcaccagtcg ctgggatttg
     1621 tcgtcaccgc acaaatgccg caggtaggca ctaaatttgg tcgttggctg gatctgacat
     1681 ttatgcagtt gcaactcgac gagcgcactg aaccggacgc gattggatga atcagtcgct
     1741 cacccttgct tttttgattg ccgccggaat tggtctggta gtccagaaca cgctgatggt
     1801 gcgcatcacc cagacctcct ctaccattct catcgccatg ttgctgaact cactggtggg
     1861 gattgtgctc tttgtttcta ttttatggtt taaacaaggc atggcggggt ttggcgaact
     1921 ggtgtccagc gtgcgctggt ggacacttat acctggcttg ctgggttcgt tttttgtctt
     1981 cgccagtatc agcggatatc agaatgtggg agcggcgaca actattgcgg tactggtcgc
     2041 cagccagctg attggcggtc tggtgctgga tatcttccgc agtcatggcg taccactacg
     2101 ggcgttgttc ggcccaatat gtggcgcaat tttacttgtg gttggcgcct ggttggtggc
     2161 cagacgctca ttttaatcag aggatggtgc cgcctttggt taactgttcg tggcgcgctt
     2221 ccatatcttc tttatgcttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct
     2281 gggtgtagcg atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt
     2341 tcatatgggc aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca
     2401 gatgcgacat actttctcct tagccacttg tgaaatttat cgattaatcc aaatgatata
     2461 agtctgcgtg gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc
     2521 accgggaaac tcatcggcca tgtggccccc accagaaagg cgcttaagaa acggatgcgt
     2581 ttcttatcgt gagaaagaaa ccaggtgata agaaatgcga ttgctgccat aaccgcataa
     2641 aaaacaagca tatgttgata gagagacatg tacgggcgtc cagccatatg aataaaacgc
     2701 gcgcaaatat gcgtcatttt ttgatgtgca tcaagttttt tcaccagttt gagaaaaaat
     2761 catcaaacag caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta
     2821 acccgtacta tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag
     2881 aactgtagta ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag
     2941 atttggctgg tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca
     3001 actctctgac agtacgcaag atcttctcat actctctagg tttgcaacat tatccttaat
     3061 atttacttct ttttgctggc atctacattg aaatgagtag aaaacatccc cctactgtcg
     3121 tctatacaaa cagcttactg attcaaacgg aatcttgttt tcacattttt ctatcacgaa
     3181 tactttatac gcgcgggagt ctataaatgc tttatacttt caaggaataa cgtaaatata
     3241 aaaagatggc gtaaagtata tgcatggcag gatgtttgca acctccttga tgacgctgcc
     3301 ccattgaaat tgcaaacccc gccctggtta tcttgcccat ttatcatgca aaacagataa
     3361 ttataggcct ctgaatacct cagaaagatt gaatttttta tagtccctct cttgaatatt
     3421 tccatttgat atcaatcgat tcatctcttc aacaaaataa tttatatcat cacacttgtt
     3481 attatccgga agatctgagt agttataatt ccaccatttt attctcaaca gttcatcaat
     3541 aac
//
LOCUS       DADLSB010000270         3493 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.271, whole genome shotgun sequence.
ACCESSION   DADLSB010000270 DADLSB010000000
VERSION     DADLSB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.271"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..432
                     /locus_tag="JN331_004116"
     CDS             <1..432
                     /locus_tag="JN331_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7262066.1"
                     /translation="PIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGIL
                     QLGVSVMSEFFPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALL
                     ALITAIIVFSRYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            444..1310
                     /gene="ydiB"
                     /locus_tag="JN331_004117"
     CDS             444..1310
                     /gene="ydiB"
                     /locus_tag="JN331_004117"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY7262067.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            1341..2099
                     /gene="aroD"
                     /locus_tag="JN331_004118"
     CDS             1341..2099
                     /gene="aroD"
                     /locus_tag="JN331_004118"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY7262068.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            2245..>3493
                     /locus_tag="JN331_004119"
     CDS             2245..>3493
                     /locus_tag="JN331_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HAY7262069.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLIIALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYAIFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCG"
BASE COUNT          913 a          798 c          874 g          908 t
ORIGIN      
        1 cccatctggg ctaatgtatt taactctgcg ctggcaacaa taacagctgc cattatctac
       61 ctgtaccctt ctccactggt gtgtaatgcc ggcgcctttg ttatcggttt ctcagcagct
      121 ggcggcattt tacagctcgg tgtttcggtc atgtcagagt ttttccccaa aagcaaagcc
      181 aaagtcacca gtatttatat gatgatgggt ggactggcta actttgttat tccgctgatt
      241 accggttatc tgtcgaacat cggcctgcaa tatatcattg ttctcgattt tactttcgcg
      301 ctgctggccc tgattaccgc aattattgtt tttagccgct attaccgcgt ttttattatt
      361 cctgagaatg atgtgcggtt tggcgaacgc aaatttagca cccggttaaa cacaattaag
      421 catagaggtt aaaggagtta attatggatg ttaccgcaaa atacgaattg attggattga
      481 tggcttatcc tatccgccac agtttatcgc ctgaaatgca gaataaagcc ttagaaaaag
      541 cgggattgcc atttacctat atggccttcg aagtggataa cgatagcttt cccggagcaa
      601 ttgaaggatt aaaagccctc aaaatgcgcg gaactggtgt gtcgatgccg aacaaacaac
      661 tggcgtgtga atatgttgat gaattaacgc cagcagccaa actggtgggt gccatcaaca
      721 ccatcgttaa tgatgatggc tatctgcgtg gctataacac cgacggcacg ggccatattc
      781 gcgccattaa agagagcggt tttgatatca aaggcaaaac gatggtgctg ttaggggccg
      841 gaggtgcctc aacggcgatt ggcgcgcagg gggcaattga aggtttaaaa gaaattaaac
      901 tctttaaccg tcgggatgag ttcttcgata aagccctcgc cttcgcgcag cgggttaacg
      961 aaaacaccga ttgtgtcgtc acggttaccg atctcgccga tcagcaagcc tttgctgaag
     1021 ccctggcttc cgccgacatt ttaaccaatg gcacaaaagt aggtatgaaa ccccttgaga
     1081 atgaatcatt ggttaatgat atcagtctgt tacatccggg actgctggtc actgaatgcg
     1141 tgtataaccc gcatatgacg aagttattgc agcaggcaca acaagctggt tgcaaaacga
     1201 ttgatggata cggcatgttg ttgtggcaag gggctgaaca gttcacgtta tggactggca
     1261 aagatttccc tctggaatat gttaaacagg tcatggggtt cggtgcctga caggctgacc
     1321 gcgtgcagaa agggtaaaaa atgaaaaccg taactgtaaa agatctcgtc attggtgcgg
     1381 gcgcacctaa aatcatcgtc tcgctgatgg cgaaagatat cgcccgcgtg aaatccgaag
     1441 ctctcgccta tcgtgaagcg gactttgata ttctggaatg gcgtgtggac cactttgccg
     1501 acctctccaa tgtggagtct gtcatggcgg cggcaaaaat tctccgtgaa accatgccag
     1561 aaaaaccgct gctgtttacc ttccgcagtg ccaaagaagg cggcgagcag gcgatttcca
     1621 ccgaggctta tattgctctc aatcgtgcag ccatcgacag cggcctggtt gatatgatcg
     1681 atctggagtt atttaccggc gatgatcagg tcaaagaaac cgtcgcctac gcccacgcgc
     1741 atgatgtgaa agttgtcatg tccaaccatg acttccataa aacgccggaa gccgaagaaa
     1801 tcattgcccg tctgcgcaaa atgcagtcct tcgacgccga tattcctaag attgcgctga
     1861 tgccgcaaag taccagcgat gtgctgacgt tgcttgccgc gaccctggag atgcaggagc
     1921 agtatgccga tcgtccaatc atcacgatgt cgatggcaaa aactggcgta atttctcgtc
     1981 tggctggtga agtatttggc tcggcggcaa cttttggtgc ggtaaaaaaa gcctctgcgc
     2041 cagggcaaat ctcggtaaat gatttgcgca cggtattaac tattttacat caggcataag
     2101 caataatatt tcggcggaaa taccctcccc cgccgaaata aaaaaagtat ttttaaccgt
     2161 attttataaa aatatttcgt gagtctctgt gcgctaattc tccatctggc gtagggaaaa
     2221 tcacacctga atcaggaatt aacaatgaaa cctgtaaaac cacctcgtat taatggacga
     2281 gtgccggtcc tgtcggcaca ggaagcggtg aattatattc ccgacgaagc aacactttgt
     2341 gtgttaggcg ctggcggcgg tattctggaa gccaccacgt taattattgc tcttgctgat
     2401 aaatataaac agactcaaac gccacgtaat ttatcgatta ttagtccaac agggcttggc
     2461 gatcgcgccg accgcggtat tagtccgctg gcgcaagaag gtctggtgaa atgggcatta
     2521 tgcggtcact ggggacaatc gccgcgtatt tctgatctcg cagaacaaaa taaaattatt
     2581 gcttataact atccacaagg tgtacttaca caaaccttac gcgccgccgc agcccaccag
     2641 cctggtatta ttagtgatat tggcatcggg acatttgtcg atccacgcca gcaaggcggc
     2701 aaactgaatg aagtcactaa agaagacctg attaaactgg tcgagtttga taacaaagaa
     2761 tatctctatt acaaagcgat tgcgccagat atcgccttca ttcgcgctac cacctgcgac
     2821 agcgaaggct acgccatttt tgaagatgag gtgatgtatc tcgacgcatt ggttattgcc
     2881 caggcagtgc acaataacgg cggtattgtg atgatgcagg tgcagaaaat ggttaagaaa
     2941 gccacgctgc atcctaaatc tgtccgtatt ccgggttatc tggtggatat tgtggtggtc
     3001 gatccggatc aaacccaact gtatggcggt gcaccggtta accgctttat ttctggtgac
     3061 ttcacccttg atgacagtac caaacttagc ctgcccctaa accaacgtaa attagttgcg
     3121 cggcgcgcat tattcgaaat gcgtaaaggc gcggtgggga atgtcggcgt cggtattgct
     3181 gacggcattg gcctggtcgc tcgggaagaa ggttgtgctg atgactttat tctgacggta
     3241 gaaacaggtc cgattggcgg aattacttca cagggtatcg cctttggcgc gaacgtgaat
     3301 acccgcgcca ttctggatat gacgtcccag tttgattttt atcacggtgg tggtctggat
     3361 gtttgttatt tgagttttgc tgaagtcgac cagcacggta acgtcggcgt gcataaattc
     3421 aatggcaaaa tcatgggtac cggtggattt attgatatca gtgccacctc gaagaaaatt
     3481 attttctgcg gca
//
LOCUS       DADLSB010000271         3464 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.272, whole genome shotgun sequence.
ACCESSION   DADLSB010000271 DADLSB010000000
VERSION     DADLSB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.272"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..1981
                     /locus_tag="JN331_004120"
                     /pseudo
     CDS             1..1981
                     /locus_tag="JN331_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            1987..2700
                     /gene="ybiX"
                     /locus_tag="JN331_004121"
     CDS             1987..2700
                     /gene="ybiX"
                     /locus_tag="JN331_004121"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY7262070.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            2774..3040
                     /gene="ybiI"
                     /locus_tag="JN331_004122"
     CDS             2774..3040
                     /gene="ybiI"
                     /locus_tag="JN331_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY7262071.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3305..>3464
                     /locus_tag="JN331_004123"
     CDS             3305..>3464
                     /locus_tag="JN331_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262072.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEA"
BASE COUNT          893 a          922 c          869 g          780 t
ORIGIN      
        1 gggtgcgttt tttgcgggtg agaacggtaa ctccaccact ggcgacgcca tttatatgcg
       61 tggtgccgat acctctaaca gtatttatat tgatggtatt cgcgatatcg gcagcgtctc
      121 gcgcgacacc ttcaataccg agcaggtcga agtgattaaa gggccgtccg gcaccgacta
      181 cgggcgcagc gcaccgacag gctcgatcaa tatgatcagc aagcagccgc gcaatgattc
      241 cggcattgac gcctccgcca gtattggcag cgcctggttc cgccgcggca cgctggacgt
      301 caatcaggtc attggtgata ctaccgcggt gcgcctgaat gtgatgggcg aaaaaaccca
      361 cgatgccgga cgcgacaaag tcaaaaatga gcgttacggc gtcgcccctt ctgtcgcttt
      421 tggccttggt acagcgaatc gtttgtatct taattatctg catgtcactc agcacaacac
      481 gccagacggc ggcattccga ccatcggttt gccgggctat tctgccccat ctgcgggagc
      541 ggcggccctg aatcattccg gaaaagttga tactcataac ttttacggca cggattccga
      601 ttacgacgat tcgaccaccg acaccgccac catgcgtttt gagcacgaca tcaacgataa
      661 caccaccatt cgcaatacta cccgttggtc gcgcgtaaag caggattacc tgatgacggc
      721 gattatgggc ggggcgtcga atattactca gcccaccagc gatgtgaata gctggaccta
      781 gtcacgcacg gcgaatacca aagatgtgag taataaaatt ctcaccaacc agaccaacct
      841 gacctcgacg ttctataccg gttctatcgg tcatgatgtc agtaccggcg tggaatttac
      901 ccgtgaaacg cagactaact atggcgttaa tccggtgacg ttaccggcgg taaatattta
      961 tcatcctgac agcagaattc atcccggcgg cctgacgcgc aacggcgcaa acgccaatgg
     1021 tcagacggat accttcgcaa tttacgcctt cgatacgctg caaatcaccc gtgattttga
     1081 gctgaacggc gggatccgtc tggataatta tcatactgaa tatgacagtg ccaccgcctg
     1141 cggcggcagc ggacgcggtg ccatcacctg cccagctggt gtggcaaaag gttctccagt
     1201 caccaccgtc gacaccgcca agtcgggcaa tctggtgaac tggaaagccg gggcgctgta
     1261 tcacctgacg gaaaacggca atgtctatat taactatgcc gtttcccagc agcctccggg
     1321 cggcaacaac ttcgcccttg cgcagtctgg cagtggtaac agtgccaacc gcaccgattt
     1381 taaaccgcaa aaagccaaca ccagcgagat tggcaccaaa tggcaggttc tggataaacg
     1441 cctgttgctc accgccgcgc tgttccgtac tgatatcgaa aatgaagttg agcaaaatga
     1501 tgacgggact tactcgcaat acggtaagaa acgcgtcgaa ggctatgaga tatccgtggc
     1561 agggaatatc actcccgcgt ggcaggtgat tggcggctat acccagcaaa aagcaaccat
     1621 caaaaacggc aaagatgttg cccaggatgg ttcctcatcg ctgccgtata ccccggagca
     1681 cgccttcacc ttatggagcc aatatcaggc aaccgatgat atctctgttg gcgcgggcgc
     1741 acgctatatc ggcagtatac ataaaggttc agacggcgcg gtgggaacgc cagcatttac
     1801 agaaggttac tgggtcgccg atgccaaact ggggtatcgg gttaatcgca atctcgactt
     1861 ccagctaaac gtttacaacc tgtttgatac cgattacgtc gcctcaatca acaagagcgg
     1921 ctaccgttat cacccgggcg agccaagaac cttcttgctc acagccaata tgcatttctg
     1981 attcagatgt ggggcgcagg ccccactttt tggagaactt gtatgatgta ccacattccc
     2041 ggcgtgttat cgccacagga cgtcgcccat tttcgcgaac aactggaaca agccgaatgg
     2101 gtggatggac gcgtcaccac cggagcacaa ggtgcacaag ttaagaacaa tcagcaagta
     2161 gacacccgca gcgcgttata cgccgcactg caaaatgagg tgctgaacgc ggttaaccaa
     2221 catgcattgt tctttgccgc tgccttgccg cgtacccttt ccacgccgct gtttaatcgc
     2281 tatcagaaca atgaaaccta tggtttccat gtggatggcg cagtacgcag ccatccacaa
     2341 aacggctgga tgcgcactga cctttctgcc acgctgtttt taagcgatcc acaaagctac
     2401 gacggcggcg aactggtcgt taatgacacc tttggacaac atcgggtaaa actcccggca
     2461 ggtgatctgg tgttgtatcc ctccagcagc ctgcattgcg tgacacccgt aacccgcggc
     2521 gtacgagtgg catcatttat gtggatccag tcgatgatcc gcgatgataa aaagcgcgcc
     2581 atgctgtttg aactggacaa aaatattcag tcgctgaaaa gccgctatgg tgaaaatgaa
     2641 gagatcctgt cgctgctcaa tctttatcat aatctgctgc gggaatggtc ggagatctga
     2701 cgcttgaact gcccaggttc gtccctatat tagctacact taactgtaca agcattgata
     2761 tggggaggac gatatggcat ccgcttgggc taacgatgac gccgtcaacg aacagatcaa
     2821 cagtacaatt gaagatgcga ttgcccgcgc tcggggtgaa attccgcgtg gcgaaagcct
     2881 gtatgaatgt gaagagtgcg gtgcccccat cccgcaagcc cgtcgggaag ccattcctgg
     2941 cgtgcgctta tgtattcatt gtcagcagga gaaagattta caaaaaccag cgtatacagg
     3001 atataatcgc agaggttcga aagacagcca gttacgttaa ctacaactgg cgagatgcat
     3061 agcgagtata atttgtattt tgcgtggcat tccgtgcctt taacgctgct gtgactgcca
     3121 cgcggcataa aacaagtcca taagtacaac aaataaatgg tttatcagta tgttagatat
     3181 caatcaattt atttgaacaa ggcggtcaat tctcttcgat tttatctctc gtaaaaaaac
     3241 gtgatactca tcacatcgac gaaacaacgt cacttataca aaaatcacct gcgagagatt
     3301 aattatgaaa actatcaata ctgttgttgc tgctatggct ctttcaactc tgtcatttgg
     3361 cgtgttcgcg gcggaaccgg taacggcatc ccaggcacag aacatgaata aaatcggcgt
     3421 ggtttctgcc gatggcgcat ccaccctcga tgccctggaa gcga
//
LOCUS       DADLSB010000272         3458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.273, whole genome shotgun sequence.
ACCESSION   DADLSB010000272 DADLSB010000000
VERSION     DADLSB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.273"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..987)
                     /locus_tag="JN331_004124"
     CDS             complement(<1..987)
                     /locus_tag="JN331_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAY7262073.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLL"
     gene            complement(1044..2405)
                     /gene="allB"
                     /locus_tag="JN331_004125"
     CDS             complement(1044..2405)
                     /gene="allB"
                     /locus_tag="JN331_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HAY7262074.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHICHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(2465..>3458)
                     /locus_tag="JN331_004126"
     CDS             complement(2465..>3458)
                     /locus_tag="JN331_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="allantoin permease"
                     /protein_id="HAY7262075.1"
                     /translation="SLPGLITFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGM
                     AIWAISLVGLGPIFDYIPSGIQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNARS
                     FREQALGQTLGLVVAYILFAVAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAV
                     LVILMTTISTNATGNIIPAGYQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDS
                     IYLFLDIIGGMLGPVIGVMMAHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVT
                     LVAVILSLGGKFIPFMEPLSRVSWFVGVIVAFVAYALLKKRTTAEKTGEQKTIG"
BASE COUNT          981 a          927 c          802 g          748 t
ORIGIN      
        1 cagcaggcaa ataacgctgc cataaataaa cgaacgccgc gtgtaatcgc cagtctgtgt
       61 taataaacca atggatgaaa caaacggtga aaatggaatt accgccagcg gtacggttat
      121 cagcgtcata aatccggtcg ccacaaagct acgacgataa cgcgtattcc ccgcgccctg
      181 ctgcggataa aaaacatccg tgccccgaat cgcaccgtag gtattgctga tattcaccag
      241 acctgtaatc accgccgtca gaataattcc cggcgacaaa gcaccgccac tgccgagcgg
      301 gaaccactgc cagtgcaact caccggagag cgaatgcgaa gaaggaaagc aaaagtacca
      361 caacaaccag ccggttatcg taccgaccag caggccataa cgggcaaaac gttgcggcag
      421 aaagataatc atcgccagta ccaggcacat caccgccacc gagagcgcga acggtggtaa
      481 ctgaatttta aaatttgggt cggctatgcc aaacggcagt ccgagcatac ctttgaaaaa
      541 gatagtggtc agctgcgcgc ccagcatcaa cataaacaag accatcaccg acggcgtaaa
      601 taaccgtgcc aggcgatggc ctaatccgct aaaaccaatc aacatcgtca gcacaccgga
      661 gagtgcaatc cctaccgcca gactggtagc gatatcgttg atcggtgtcc cgcgcgatgc
      721 ttcgccaagg gtgatagtaa ggatggttcc ccaccacagg ccacctggcc cttccataat
      781 agcgcgacga tgaccgcaaa acgcctgagc gaagcaggcc agtgcggtag caagaaaagc
      841 atattgcgtg agcgtaagca ggctactttg cggcaactga aaagcagaaa gtagcgtagg
      901 aggaaccaca accgtgttgc aaaaaataaa gaaaaaccac tgaaatcctg ataacaggct
      961 ttcgcggctg actgcaaaat tgaacataaa cacaccttaa ccggagaatg cccgccgcaa
     1021 ggacgggcag tgcaggggcc agattactgc tgatgtttaa ggataaattg acctttcggc
     1081 gcaacaggga agccctgttc aatgtcgtaa atcacatcac cacgtaagat ggttttcgtg
     1141 atacgcgcgc caatggtacg gccaacatac gggctgactt tgtggcgata ttccagatcg
     1201 tcattggtaa gaacatagct gctattcggc tgaatgaaga cgaagtcggc atcttttcct
     1261 ggggcgatac ggcctttttg ctgcagaccg aaaatatctg ctgcgttagt cgccattaat
     1321 ttgccgaaca ttggcagaga cattccgcgt ttctgtaccg cttcatcgaa catcacgtcc
     1381 atacagtttt gcagaccagc aataccgccc catgcttcca tgatgttgcc ggctttcatt
     1441 tccggagggc atggtgagtg gtcggaaacc aggcagtcga tttcaccgtt aaacagtttt
     1501 tcccacatgc ctttctggtt ttccagatcg cggatcggcg gtgaacactt cgccagagta
     1561 ccgatttctt cgaactgatc ggtatccagt acaaagtaat gcgggcagga ttcacaggta
     1621 acatcctgac cttcctgacg tgcacgagtc acttcttcaa caccttccgg gctgctgata
     1681 tggcaaatgt gcagacggca accggcaact ttcgccaggt acagtacgcg gcgaatcgct
     1741 tccacttcgg taaataccgg acgcgaagcc acatagtcat gggcagttac gcgaccttca
     1801 cgtttcgctt cttcgcccag tgcatcacag atcagcgcgt tttcgcagtg caccagcacc
     1861 ggctgcccca gttcgcccag cttctgcgca cctttgaaga actgccagtc gtttacatca
     1921 cggaagtcgt tgtcgatacc gcgatcgcca caggtcgcaa cgaagcattt gaagccgaca
     1981 acgcccactt catccaactc atgcagacga tcaatgttgt aagacaccag gccaccgagt
     2041 tgtgccgcat cgatagtcag cttgccttta gcggcatcga acttcagttc aattgaagca
     2101 cggtcaaccg ttgcaggcag ctggttgagc ggcatttcga tcatggtggt gataccacct
     2161 tttgccgctg cgcgagtacc ggtttcataa ccttcccagt ggctacgacc cggttcagaa
     2221 atatgggtgt gcgcatcaac catgcccggc gaaaccacca gaccagacgc atccataact
     2281 tcttttgcat cgcccagatc ctgaccgata gcagcaattt ttccgccttt aacggcgata
     2341 tctacaacgc gagcttcgtt ttctaaaata acggtgccgt ttttaatgat taaatcaaaa
     2401 gacataacaa actccttgta aaatacagat tggtttttaa ctcaatgttc aatatcggga
     2461 ttaattaacc tatggttttt tgctctcctg ttttttctgc tgttgtacgt ttctttaata
     2521 aggcgtaggc cacaaaggcg acgatgacgc cgacaaacca tgaaacacgc gataacggtt
     2581 ccataaaggg aataaactta ccgccaagag ataaaataac ggccaccaga gttactgaaa
     2641 acgcagtgag gttaaaaccg ttatcgtaat atttataatc gccaggtgcg gtatacagtt
     2701 catcaagatt aattttccca cgcatcacca caaaataatg cgccatcatg acaccaatta
     2761 ccggaccaag cattccgccg ataatatcga ggaacagata aatactgtcc tgattttcca
     2821 ttaatttcca cgggcagatc agcaggctga taatactggc aatcagtacg ccgtttttat
     2881 aggtcagttt cgtcggtgca atagcggcaa tctgataacc ggcaggaata atattaccgg
     2941 tggcgttagt ggagatagtc gtcatcagaa taaccagtac cgcaaagaac gaggcgaaca
     3001 agctgtccca acgctgaaca atatccagca cgttccaggt atcagcgccg taatgaatac
     3061 tggctccggc aataatacat acccccgcga ccgcaaacag aatataggcc acaactaaac
     3121 ccagcgtttg ccccagcgct tgctcacgaa acgagcgggc gttttgcgta aagtcggatg
     3181 cgctcaccgc cggtgccgcc cagaccgcaa ctaccgcgtt tatcaccacc aggaacagga
     3241 agccactgtt ttctgctttc tgaataccgc tcggaatgta gtcaaagatt ggaccgagcc
     3301 cgaccagcga aatcgcccaa atcgccatac cgccgaaaac gatatagatg cacgggttaa
     3361 gaatggcagt gaatttattt aaaactttgc caccgccaaa acctatacca acgttgacca
     3421 gccagaagag taagaaagta attaagcccg gtagagaa
//
LOCUS       DADLSB010000273         3420 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.274, whole genome shotgun sequence.
ACCESSION   DADLSB010000273 DADLSB010000000
VERSION     DADLSB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.274"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..157)
                     /locus_tag="JN331_004127"
     CDS             complement(<1..157)
                     /locus_tag="JN331_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7262076.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKKGN
                     MFNQLLAS"
     gene            complement(154..444)
                     /locus_tag="JN331_004128"
     CDS             complement(154..444)
                     /locus_tag="JN331_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7262077.1"
                     /translation="MVDLRKAARGQMCTVRIPDYCNHDPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(444..1043)
                     /locus_tag="JN331_004129"
     CDS             complement(444..1043)
                     /locus_tag="JN331_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7262078.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPSEQKLVSGFVEYLYESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1110..1370)
                     /locus_tag="JN331_004130"
     CDS             complement(1110..1370)
                     /locus_tag="JN331_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262079.1"
                     /translation="MKIEELREIFSEDGLYTVCVENDAIVSHCCIKCLQFQQRKSGVA
                     LIYFADGLVTDGFLLRGDEFVTSLRALKETGLKAGFSAFECE"
     gene            complement(1594..1806)
                     /locus_tag="JN331_004131"
     CDS             complement(1594..1806)
                     /locus_tag="JN331_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7262080.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIATALVT
                     RKDLCEVRIRTGQTEVAVFVDYESRE"
     gene            complement(2022..2567)
                     /locus_tag="JN331_004132"
     CDS             complement(2022..2567)
                     /locus_tag="JN331_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7262081.1"
                     /translation="MNSVSVENLPSLTHQHLPVITTELLAELYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDELRELKNRNSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDSYFNQYEKERATGKMNIDIHLAIRDDKTAFIRSYAPGQLVTVEEALML
                     LQSRGWLVMPRDELAGKLMEL"
     gene            3101..3388
                     /locus_tag="JN331_004133"
     CDS             3101..3388
                     /locus_tag="JN331_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HAY7262082.1"
                     /translation="MAQVYFDTLKFVETLEAAGMPAAQARAISAAVKDSHEAAEVATK
                     RDLNDVRTELKNDIAALDAKIDKLSLQLTVRFGGMLVVAVGALTAIIKLPL"
BASE COUNT          831 a          902 c          774 g          913 t
ORIGIN      
        1 tggatgccag caactggtta aacatatttc ccttcttacc gccgccaata tacgaccagc
       61 gcccccacat gcgcagtttt ccctgaatcc agacactttc cagcgtggtg agacgaaggt
      121 gttccccgct ttttcctgta ttcgttgggt aaatcacaaa tatccctcct ttctccagat
      181 ttcttgcgtg cgaaaaacac cttctgcatg catcaggcgt aattcttctt tggtgtaatc
      241 gcttgttttt acccgcccgt cgattaaatc gtggcatgag ctacaggcaa ttgctgcctg
      301 catatcgtgt ggttttgtcg ctgttccgca cgtccccgcc agcctgtaat gcgccagcac
      361 agaagtttcg ggatcgtgat tgcagtagtc aggaattctg acggtgcaca tctgcccccg
      421 cgccgcttta cgtaaatcca ccattacgca aactccagta gctgcgcggc cacattttcg
      481 acttgttccg gagaggaaaa tttacggaac aggatccagt tccacagcac attcagcaca
      541 gatttataaa cctgctgaaa ctcggtttcg tccatgttcg caaatgcgat agattttgcc
      601 ctgcgcccac gactaccatc aggataaata tgctcggtgt aaaatccggc ctgaatggtt
      661 acccactcgc ggaaagcctc aaacgacttt agcaatgccg tatcccgggt tctgcgtgtc
      721 gcaacggtat tcagatattg ctctgcggca tcactcaagg ctggagtgtg ttccctacct
      781 actgattcgt acaggtactc aacgaagcct gaaaccagtt tttgttcgct aggcgtgatc
      841 gcccctccga ccggagtcca gtaatcgaac cccagttgca ggagtttgaa aaaacgcttg
      901 tggaatgcgt agttacgaac gcgcttaaaa tcagcgtgta tccactcgcc tgttttgatt
      961 tgatgcagaa attcgcaact ctccggcgtc gccgggagaa gtaatccgga agaggtttgt
     1021 ttgaccagtt gtatatgtgc catcggcttt ctccggtggc acggtgttac tcagcagggg
     1081 ggcaaccctg cactgaattg tagatgaact cactcacatt caaaagcaga aaagccagcc
     1141 ttaagtccag tctctttcag cgcccgtaat gacgtgacaa attcatctcc acgcaaaaga
     1201 aaaccgtccg tcacaagtcc gtccgcgaaa taaattaacg ctactccact ctttctttgt
     1261 tgaaattgta aacatttaat gcagcagtga ctgacaatag cgtcattctc aacgcacaca
     1321 gtatagaggc catcttcact aaaaatttca cgtaattctt cgattttcat caacagaatc
     1381 cttccagata aatagctctc ccctgttcgg ggtccacccc tcttctccct gcgcgctact
     1441 taagtgtttt tgattctatt cctgcgcatt ctgaaagttc aaacgcgttg aaaatcgcaa
     1501 caaaaaaccc gccgaagcgg gttaagtgcg ggtgcgttga ggatgcctga cacatcagag
     1561 gtggcgaggg atttctccct cgcctggtca ctcttactct ctagattcgt agtctacgaa
     1621 gacagcgacc tccgtctggc cggttcggat tcgtacctca cagaggtctt tcctcgttac
     1681 cagtgccgtc gctatgacgg ttaaacagat gacgatcagg gcgattaaca tcgccttttg
     1741 ctgcttcata gcctgcttct ccttgccttt cggcacgtaa gaggctaacc tacatgtgtt
     1801 cagcatggat tgagcctcag attaatgtta agcgtcttgc aggacgcgta atgttaactg
     1861 gggcttttct ctatctgcct tttggtgttc atgcctgagg cagatagcct caggcacccg
     1921 cagcaattct acttgacttc cctttcaccg caaatcattt tatcctcggt ggtaatgttc
     1981 tcccgatatg ggaattccca tatcggggct aatttaacca gttaaagctc cattaatttt
     2041 ccggcaagtt catctcgcgg cattaccagc catccgcgcg attgaagcaa catcagggct
     2101 tcttcaaccg tcaccagctg accaggcgca taacttcgga tgaaggcagt tttatcatca
     2161 cggatcgcca ggtgaatatc gatgttcatt ttccccgtag cccgctcttt ctcgtactgg
     2221 ttgaagtagc tgtcttccag tttttcgaat acttcccacg cctgatcggt ttcgagcatt
     2281 ttggcgtgac gggctgcacc tcgttctgtc cagaggatga gtgagcgaac attgcgggcg
     2341 attttcacag aaactcttaa agagtttctg ttcttcagtt cccgtaattc atcgccttcg
     2401 atcaggaaaa agtgttttcc ctctataaac cgctctttgt ttctattgaa attgtttgtc
     2461 aaacgctggc gctctgttcc gtacagctca gccagaagtt ctgttgttat aacaggaaga
     2521 tgttgatgtg taagtgatgg gaggttttca acagatacgc tgttcatgaa tatgtcctac
     2581 tgaatatttt gatttacccc ttttgagagg gtgaccgggc gctcaaaacc gtcagtagtc
     2641 ggcgggcgta ttcccattgc tggtattgta ttagccgcac gcccagtcat aaaccaagaa
     2701 ttctggacac aaaaaaaacc gcatgtctgt cgggcgcggt aaccgctact gaaaggtgtt
     2761 ttgagcaccg tcgaggaatg taatctgcta acatgaaact gtcaatacat tcatgttcca
     2821 atcatcgggt ggcgaactgt gcggggttag cactaccggg tacggaaacc ggcgagcctt
     2881 tcggctcccc cacacagccc gccataaatc gcgaatgtga ctatgcaaac gatatgaaaa
     2941 aagacgcggg cgcgtctcat atcgctccgt aaacatccgg ggtgctaatc ccgacgccag
     3001 attttgctgg cgcgtgagga atatagcccc ggattaacgt tgtcgtcaac agccactaca
     3061 ggctaaacta acaatattcc ccaacaacga aggatttaac atggctcagg tctattttga
     3121 tacactgaag ttcgtcgaaa ccctggaagc cgcaggaatg ccggcagctc aggcgcgcgc
     3181 tatatctgcc gcagtgaaag attctcacga agccgcagaa gtcgccacaa agcgcgacct
     3241 gaatgatgtc agaactgaac taaaaaacga cattgcagcc cttgatgcaa aaatagacaa
     3301 actcagcctg caactgaccg tcagatttgg tggcatgctc gtcgttgctg ttggcgcact
     3361 gacagccatc atcaaattac ctctttaaat taccagcccg ttcggggctg gtttacctta
//
LOCUS       DADLSB010000274         3383 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.275, whole genome shotgun sequence.
ACCESSION   DADLSB010000274 DADLSB010000000
VERSION     DADLSB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.275"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..248
                     /locus_tag="JN331_004134"
                     /pseudo
     CDS             1..248
                     /locus_tag="JN331_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            272..679
                     /locus_tag="JN331_004135"
                     /pseudo
     CDS             272..679
                     /locus_tag="JN331_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1264..1980
                     /locus_tag="JN331_004136"
     CDS             1264..1980
                     /locus_tag="JN331_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262083.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGSYEINDVYVRK"
     gene            2532..2936
                     /locus_tag="JN331_004137"
     CDS             2532..2936
                     /locus_tag="JN331_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262084.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2999..3383
                     /locus_tag="JN331_004138"
                     /pseudo
     CDS             2999..3383
                     /locus_tag="JN331_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          969 a          683 c          783 g          948 t
ORIGIN      
        1 actggcagag atagctgtcg ctggcggcca acagtgacgt ccccatgatg aagaatgtgg
       61 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catacgccat ggcgtatcaa
      121 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      181 accgcaaccg ggagcgcttt aagctgggag tgatgttcca ctatgggaaa ctgaatatgg
      241 cgttctaagc cttcccacca tgatcgggga agacccagat atgaacaggc acattcagag
      301 ttactttaaa gagcttactg aacgtctgag gtgttagagc catgactgcc gcagcattgc
      361 tggcggaaat gcctgaatcg agcagcctct cgagacgtga aatcagcgca cttgtcggtg
      421 ttgctcaggt aaaccgggac tcgggaactc tgcgcggtcg acgaaccatt ttcggtgatt
      481 gtgctgggga agaacagctc tgtacatggc ggcgcttgag gcaccccggt ttaatctggt
      541 gataaaggcg ttttacatgc gcctacttgc agccggaaac gccaaaaaag tggcactggt
      601 tgcctgtatg cgtaagttgc tgaccattat gaacgcaatg ctcagaaaaa acgaagaatg
      661 gaatgaatcg tatttatgaa aactggattg tgggccataa atattccaaa aaaatagccc
      721 gctccgttta ttaccggaac gggcgagagc atgacattgt ctgtaattat aatgagactg
      781 ctgattctac ttatttttaa tttcaagaaa agcgttttct acaaagaatt atgagattta
      841 atgttttgtt tagacaatat tatcattctg ttcaataatg aaagtcttca tcaacaacaa
      901 gtaaaaacaa cgtccctctc atcgaaccgg acttgcagat ttcccgcatc cggctctctt
      961 gcaacacatc agaccttcac gttcgggcgg ttatggggaa ggtccagcaa gctgtatgag
     1021 ccctaactgt ttgaatacca gtagattacc agaaacttct atcgattgaa gacattacca
     1081 actagcgtta ctaaatgttg accaagtttt gttcacttgt ctggttttga tagtagcatt
     1141 ctgcagagta ttttgatatg ataaatatgc attaacaatg atattattgt taatttccta
     1201 gcatgaaaat taaaagatca actttttatc tcgaatattt tttacattat cagttggttt
     1261 cttatgaatg acaattcatt gcttagaaat tcttcattat tcatcgccta tatgggatgt
     1321 gtagggtggg tcagtgctta ttcttatggc tggggaacct ctttttacta tggatttcca
     1381 tggtgggttg ttggtgcagg tttggatgat gttgccagaa gtttactcta tgctatcata
     1441 gtaatgggta tactttttac cggttggggc attggtattt tattttttct tcttataaag
     1501 aaaagaagta aaatacaaga cttgagtttt tttagactat tttttgctat taccctactg
     1561 tttttccctg taatattcga acttttaatt ctcaaacaat atttcatact tccattatca
     1621 ctttcatgca ttattagttc attggttatt tcaataataa ttcgtatata tggacggatc
     1681 ttttctgtat catgtttttc agatatccct tttgttagag aacaccgcat taagttaatt
     1741 atggctggtt ttttggttta tttttggctt ttttcctttc ttgttggctg gtataaacct
     1801 caattaaaaa aagaatatca gatgctttgt tataataata gttggtatta tgttcttgct
     1861 cgttatgatt cacggctcgt tttgtctagt tcatttaaag atgacagtaa cagattctta
     1921 atatttaata cagaacagtc gggatcttat gaaatcaatg atgtttatgt cagaaagtaa
     1981 taatcctctc ccacaaagtg aattaaatga atatataatg cgttcgaagt atatgtattg
     2041 agctgtcata ctgagaaaca aaccttaggg cagcacaatt aaagccagaa acaactatgc
     2101 aagaaagtta ctaaataagt gctcggaaca gtaaaacact caggggggac tcagtccaga
     2161 ttatgtaatc agataaattg ccaaacatca caaatcacca gccggactga gtgatgccga
     2221 gcattgcacc aataccccgt gatgaacgat gcctgatgca gaaagctccc tataaaacaa
     2281 agaacaaaaa ctacgaccgg cgattcaccg tcatactgat gctgcatcgt ggtgacactg
     2341 tctgtcactt tcatgttgac tgaacaactc tgctgcgaag aaggtgtacc gccggcggga
     2401 tttgattgtc cggcgtctag cttccacttc cctgatggaa ctgaaagttg ttatggccgg
     2461 tcagcttgcc ctgtccgcag gacatgaggt gctgtgacgg acagcaccag taacggaaag
     2521 tgacaatgaa aatgaatact gcgctgatag ttgcactaat gtgtatgtgg tatgcggtac
     2581 ccgctgctgc aaaagagact ttactggcta tgccacgtaa ttcaacagaa cattgctatg
     2641 cggagattaa tgtgcacgga ccatatgggg tgtatttccg agtggtcccg catccccccg
     2701 ggggaaagag ctgggtcgaa tgtaacagtg actactatta ctctgataaa ccaccgggag
     2761 tacagatact cggaacccgt gcagggtgtc gggtatacgg aatctgcggg accacctcga
     2821 cactgcatgt ggctggaaga ggggttgtgt gtattaaaaa tatatgcagt ccacgtggga
     2881 tgataattca caggatacgg aaacgtcctg tagtggctgt aagcgatgag atgtgatctt
     2941 acccagcaat agtggacacg cggctaagtg agtaaactct cagtcagagg tgactcacat
     3001 gacaaaaaca gtatcaacca gcaaaaaaac acgtaaacag cattcacctg aattttgcag
     3061 tgaagccctg aagcttgctg aacgcatcgg tgttgctgcc gcagcccgtg aactcagcct
     3121 gtatgaatct cagctctatg cgtggcgcag caaactgcag caacagatga cctcgtctga
     3181 gcgtgaaagt gaactggccg ctgaaaatgc ccgcctgaag cggcaactgg cggagcaggc
     3241 agaggaactg gctatcctcc aaaaggccgc gacatacttt gcgaagcgcc tgaaatgaag
     3301 tatgtcttta ttgaaaaaca tcaggctgag ttcagcatca aggcaatgtg ccgcgtgctc
     3361 cgggtggccc gcagcggctg gta
//
LOCUS       DADLSB010000275         3319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.276, whole genome shotgun sequence.
ACCESSION   DADLSB010000275 DADLSB010000000
VERSION     DADLSB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.276"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..858)
                     /locus_tag="JN331_004139"
     CDS             complement(<1..858)
                     /locus_tag="JN331_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY7262085.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGQVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQCFFRDHNI
                     TIEMTESDAE"
     gene            complement(877..2754)
                     /gene="bglF"
                     /locus_tag="JN331_004140"
     CDS             complement(877..2754)
                     /gene="bglF"
                     /locus_tag="JN331_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY7262086.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2888..>3319)
                     /gene="bglG"
                     /locus_tag="JN331_004141"
     CDS             complement(2888..>3319)
                     /gene="bglG"
                     /locus_tag="JN331_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY7262087.1"
                     /translation="EALTIIDKRLGVQLPKDEVGFIAMHLVSAQMSGNMEDVAGVTQL
                     MREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASINDSDESLQQAVKQNY
                     PQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT          871 a          897 c          752 g          799 t
ORIGIN      
        1 ttctgcgtca ctttcagtca tctcaatggt gatattgtgg tcgcggaaga aacactgcat
       61 atagccggga tactggccac gcgcctgaac atcaccaaag aacatccagc gccggttctc
      121 ttccatggcc tgcaacatat cctgtggctg gcaggtgagg gggtaaacct gcccaccgag
      181 aagcatattg ccgattttcg cttcggggat caggctatga taggctttaa ctgcccgcgc
      241 actggcaacc agttgatgat ggatcgcctg ataaacttcc gcctcgccac tctcttctgc
      301 cagccccacg cccgtgaatg gcgcgtgtaa cgacatattg atttcattaa acgtcagcca
      361 taacgctact ttatgttggt agcgggtaaa gaccgtgcgg gcgtaatgct cgaagtgatc
      421 gatgaccgcc cgattagccc aaccgccgta gtttttcacc agcccatata gcatttcgta
      481 atgggataac gttaccagcg gcttgatccc cgcctgcgcc atttcatcaa acagccgatc
      541 gtaaaacgct aaccccgctt cattcggttc ggcttcgtcg ccctgaggga aaattcgcgc
      601 ccaggcaatg gaaatacgca gacaggtgaa gcccatctcg gcaaataacg cgatatcttc
      661 cgggtaacgg tgataaaaat cgatggcgac atctttgata ttctctttcc ccaggatgcg
      721 cggttccatt tttcccatta cgccatgagg ctgtaaatct gaggtcgtga tccctttgcc
      781 atcttccagc caggcacctt ccacctgatt ggcagctgtt gcgccacccc aaagaaatgt
      841 ttctggaaat gctttcataa ttaactcctt ttatcgttag cgaatgatgg ataacagcgg
      901 ttcacctgcg cttatctgcg ccgtgccgtg gggtaatacg tctgtaaaat catcgctatt
      961 actgattaat accggcgtcg tcagatcaaa tccggcctcg cgaatagcag ggatatcaaa
     1021 agaaatcagc cgatcgcctg tattgacctt gtcacccacg ttgacgtgag cggaaaagaa
     1081 tttgccgtcc agttttacgg tgtcgatacc gacatgaatc aggatctcca caccatcatc
     1141 tgactcaatg ccaatggcgt gtaatgtggc gaacaacgaa gcaattcgac ccgcaaccgg
     1201 agaacgcact tcaccaaccg agggcagaat ggcgatacct ttacccaaca ggccactggc
     1261 aaacgtggta tcagcgacgt gaatgagcgg cacaatctct cccgtcatcg gtgaacagat
     1321 accgccctgc tcaggtggtg taataacctc tggtgttttc tcttgcgggg caccctgcgc
     1381 tggctgacgt ttactggtga tgaaatgaag catcaacgta ccaacaaatg cgcaacctat
     1441 ggcaatgaca ccgccaataa cgctggccca gacggtgaaa tcaatccccg ttgacgggat
     1501 ggtctgcatg aaggtgaaaa tacttggcaa accaaaggag taaactttcg tttgcgcgta
     1561 gccaatgatg gtggccccca atgctccact gatacaggcg ataacaaagg ggtacttacg
     1621 cggcaggttg acgccatata ccgctggttc ggtgatacca aacagacccg tcaacgccgc
     1681 tgatcccgcc accacttttt tctgcgcatc gcgttcgcag aggaagacgc cgagcgccgc
     1741 cccgacctgc gccataatgg cgggcattaa cagcgggatc atggtgtcgt agcccagcac
     1801 ggtgaagtta ttgatacaca gcggcaccag gccccagtgc agtccgaaca tgacgaagat
     1861 ttgccagaag ccgcccatta ccgcgcccgc aaatgcagga accgcctgat aaagccagag
     1921 ataaccggcg gcaatcattt cgcttatcca ggttgatagc ggccccacca gcagaaaggt
     1981 gacgggtgtg ataaccatca gacatagcaa tggtgtgaag aaatttttga ttgccgacgg
     2041 taaccacgca ttaagtcggc gttccagaat gctgcacaac caggcagaaa aaataatggg
     2101 aataaccgat gacgagtaat tcaacaatgt gaccggaata cccaggaaat ccagccccgg
     2161 cgcatccgct ttttgcccgt tctcgaaggc agtcagaatt aatggatgca ctaacgctcc
     2221 accaatcacc atggcagtaa atggattacc gccgaagcgt ttgcccgcgg tgtatcccag
     2281 gattatcggg aagaaccaaa acaaggcatc actggcgctg aataaaatta aataagtacc
     2341 actttgttcg gtcgtccact gaaaagtgag cgccagagcc agcatacctt tcaggatccc
     2401 ggttgccgcc atcagaccga tcagaggcgt aaaaatacct gaaataacat aaacaaagcg
     2461 gtttagcaga ttacctttct catcattttc cggtgcctgt tgcgcttttt cgccaaggcc
     2521 tgccacgctg ttaaccgcca ggaagacatc ggccacatgg ttacctatga ccacctgaaa
     2581 ctgaccaccg ctttccacca ccataataat accgggggtc tttttcagta cctccgcttg
     2641 cgctttgctt tcatccttta acttaaaacg taatcgcgtt gcgcaatgca tcagactcac
     2701 aatgttatct gcgcccccga ctcctgcgac tatttttctg gctaactccg tcataacttg
     2761 ccctctaccg ctttgcggca aaactccaaa aaaaaacctg aaaaaaacgg cctgacgtga
     2821 atcaagcaat ttttttcagg ttttgcccgc ttagtgcggt aacaatcctt tactcagtaa
     2881 taatatttca gtgttctttg cgcacgcgct ctatatttat ggctaaaaac ataatctctg
     2941 cgggtgaaat tttacgttga tactgcaaac caataaaaat ggcgatccgt tccgcacatt
     3001 gccatgcttg cgggtaattt tgttttactg cttgttgtaa tgattcatca ctatcgttaa
     3061 ttgaagcatg ttcaagaata cgccaggata aaaacttcag atgtgtaacc agtcgctgat
     3121 aactcaagct ttcttcctgg taattaaggc tgaactgaaa ttttattaat tgcagcattt
     3181 cgcgcattaa ctgcgtgaca cctgcaacat cctccatatt tccgctcatt tgggcactga
     3241 ccagatgcat ggcaataaag cccacttcat ctttcggtaa ctgcacgccc aaccgtttat
     3301 caataatggt taacgcttc
//
LOCUS       DADLSB010000276         3281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.277, whole genome shotgun sequence.
ACCESSION   DADLSB010000276 DADLSB010000000
VERSION     DADLSB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.277"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            143..391
                     /locus_tag="JN331_004142"
     CDS             143..391
                     /locus_tag="JN331_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262088.1"
                     /translation="MNNPIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            724..2247
                     /locus_tag="JN331_004143"
     CDS             724..2247
                     /locus_tag="JN331_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7262089.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2379..2598
                     /locus_tag="JN331_004144"
                     /pseudo
     CDS             2379..2598
                     /locus_tag="JN331_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2998..3207
                     /locus_tag="JN331_004145"
     CDS             2998..3207
                     /locus_tag="JN331_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262090.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT          910 a          642 c          700 g         1029 t
ORIGIN      
        1 gtgttgcgat acacagctga tgatatgtaa cttctaatta ttaagtataa gtttatagaa
       61 aactcattca tcttattttt gtctgtcgct ttagactata cacagcataa ttttattggg
      121 ttaatatttc tacgaggctg acatgaataa tccaatccca gagagattta tttttcaatg
      181 tgctttgttt aaaaatctcg agagggaggt gttcatgacg catgggtatg ttgatagtca
      241 tattattgat caggctttac gcttgcgttt aaaagatgaa accagtgtga ttctttctga
      301 tctctatctg caaatattgc agtacattga aatgcataag actacactaa cggatatcat
      361 tattaatgac agggaatccg tgctctctta gaacttgagt aaggttttag aataaacatc
      421 agctgtatca ccatgctgat gcaaagtgag catttcaggc gttatgcttt cttattatgt
      481 ccgcaatatc aggtgtcaag aatggagagt tctcgctctc cattcttgac acctgatatc
      541 ccgcctaact tattttgtac tgcctacatt atgctcaata attaatgggt tagatcaaga
      601 tggagaaaat tttatcttcg gcgtggatat ttcttatgct ctctaccatg catctctgta
      661 gatgccagcg cgagcgaatg aatggcattt tcatgtatta aaccctttgg ccgggacaag
      721 caaatgcgca atacactcat acccatcctc gttgcgatat gcttgtttat taccggtgtc
      781 gctattttga acatccagct ctggtattcc gcaaaagcag agtacctggc gggagcaaga
      841 tatgccgcca acaatatcaa tcatatactt gaagaagcgt cacaggcgac tcaaacagca
      901 gttaacattg ccgggaagga atgcgacctc gaggagcaat atcagcttgg cactgaagca
      961 gctctgaaac ctcacctgcg cacaatcatc attctcaaac agggaatagt ctggtgtaca
     1021 tccctgcctg ggaatcgggt cctgctgtct cgtattcctg ttgtcccgga cagtaattta
     1081 ctgttggctc cggcaatcga caccgttaat agattaccta tcctgctcta tcagagccaa
     1141 tttgcagata cgcgcatttt ggttacgata agtgatcaac atattcgcgg ggcgcttaat
     1201 gtacccttga aaggggtaag gtatgtatta cgcgtggcgg atgacattat tggaccaacg
     1261 ggtgatgtga tgacgcttaa tggacattat ccctataccg agaaggttca ctccacaaaa
     1321 tatcatttca ctattatctt taacccgccg ccactcttta gcttctacag acttatcgat
     1381 aaaggctttg ggattttaat atttattctg ttaatcgcct gcgccgctgc cttcctgctt
     1441 gatagatatt tcaataaaag cgcaacgcct gaagagatcc tgcgacgggc aataaataat
     1501 ggggagatcg tcccttttta ccaacctgtg gtaaatggtc gggaagggac attgcgggga
     1561 gttgaggtgt tagcccgctg gaaacaacct cacggtggat atatttcacc cgcggcattt
     1621 attccacttg ctgaaaaatc cggattaatc gttccgctta cacaaagcct gatgaatcag
     1681 gttgccagac agatgaacgc tatcgcgagt aaactgccgg aaggttttca tattggaatt
     1741 aattttagcg cttcgcatat tatttcgccg acgtttgtcg acgagtgttt aaatttccgt
     1801 gacagtttta cccgccgcga tttaaacctt gttctggaag tcaccgagcg tgagccattg
     1861 aatgttgatg aaagtctggt tcagcggttg aacattctgc atgaaaatgg ttttgtcatc
     1921 gcgctggatg atttcggtac tggctactca gggctttctt atcttcatga cctgcatatt
     1981 gattatatca aaattgatca cagtttcgtt ggccgcgtaa acgcagaccc agaatcaacc
     2041 cgaattctgg attgtgtatt ggatctggcg cgtaaacttt cgatcagtat cgtcgctgaa
     2101 ggtgtcgaaa cgaaagaaca acttggctat ctgaaccaaa ataatatcac atttcagcag
     2161 ggttattatt tctataaacc tgttacatac atcgacctgg tcaaaattat cctttctaaa
     2221 ccgaaggtga aggttgtggt tgagtgaaaa ttgatcagta aggccatagt gcggtgtaat
     2281 tatagacagc taattagctc gttgcctctt gttactattg ttcattattt tgtttgctat
     2341 aattgtttga aagttttgac aggattgcca ttagtagcat gaacaatagt aataatctgg
     2401 attgtttcac tctctatatc atcttttcca ttgcatttat gctgatcacc ctcctggtca
     2461 tccttattgc aaaacccagt accgggctgg gagaagtgct tgtgacgata aatttgctta
     2521 atgcccttgt ttggctggcg atcaatctgg ttaatcgatt taagagaaag actcgtcagc
     2581 cacagggatc agcaataatc tttcagtttc tcactgtcag tatgcggctg aatgggttgc
     2641 tggcagtgaa cgcctggatc attgaaggaa aggaattatt gcgcaaatag ttgtcaacct
     2701 tggtgttatc acagttgttt ttatatatca ccgaaataat taccatggca actattaata
     2761 attttgatgt cgaagagtta tttgttaaac aaaatagtca ccccaaagtg atcaatgtca
     2821 tgaaaataag gtgaaaaatg ataatgccga cttatttatc atttatatat tgtcgctgtt
     2881 tatctattta aattttttgt gcttttgttt ttgtctttac cctccgtttt ggtaatctga
     2941 gctcataggt tatttgacca ggaatgtttg ttaatccaat gaatggatgg ttaatctatg
     3001 aaactgaaaa aactccccgg gtttagtttg ggacttattg ctctggcggt gggtaatgca
     3061 tatgctgaac agtttattca gcacgatgaa tataaacatg ctgtggcaat aaaagatgga
     3121 aaagtaaaag atattgataa tgcgctagtt ataacaacgg gtaatggaag ttacgggtat
     3181 ccgttgcagg agagggtagc aagttaacca ttaacaatgg cgtcattgta acaacgggcg
     3241 gtttatatcc ttctgacaat agtcctctaa gcccagcatt a
//
LOCUS       DADLSB010000277         3264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.278, whole genome shotgun sequence.
ACCESSION   DADLSB010000277 DADLSB010000000
VERSION     DADLSB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.278"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..263)
                     /locus_tag="JN331_004146"
     CDS             complement(<1..263)
                     /locus_tag="JN331_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262091.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSV"
     gene            288..527
                     /gene="relB"
                     /locus_tag="JN331_004147"
     CDS             288..527
                     /gene="relB"
                     /locus_tag="JN331_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HAY7262092.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            527..814
                     /gene="relE"
                     /locus_tag="JN331_004148"
     CDS             527..814
                     /gene="relE"
                     /locus_tag="JN331_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY7262093.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            886..1041
                     /gene="hokD"
                     /locus_tag="JN331_004149"
     CDS             886..1041
                     /gene="hokD"
                     /locus_tag="JN331_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY7262094.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1259..1510
                     /locus_tag="JN331_004150"
     CDS             1259..1510
                     /locus_tag="JN331_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262095.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVASLQALKEIGIKAGFSAFSEE"
     gene            1577..1855
                     /locus_tag="JN331_004151"
     CDS             1577..1855
                     /locus_tag="JN331_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262096.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            1857..2906
                     /locus_tag="JN331_004152"
     CDS             1857..2906
                     /locus_tag="JN331_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7262097.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLLFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            2919..>3264
                     /locus_tag="JN331_004153"
     CDS             2919..>3264
                     /locus_tag="JN331_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7262098.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYP"
BASE COUNT          831 a          772 c          871 g          790 t
ORIGIN      
        1 aacactgaca tgctaaagca aaaaatagat gaataagttg agttgtgcat atgtagcctg
       61 accgtcacaa agtatatggt gtctgtacca gtaagatgat ggccggactc tttaaaaaag
      121 agctgacctg cacaatacag gatggactta gcaatggctg ctcctggcac aaagcggaca
      181 gtgatcaccg ttcttacgac tactttctga cttccttcgt gacttgccct aagcatgttg
      241 tagtgcgata cttgtaatga catttgtaat tacaagaggt gtaagacatg ggtagcatta
      301 acctgcgtat tgacgatgaa cttaaagcgc gttcttacgc cgcgcttgaa aaaatgggtg
      361 taactccttc tgaagcgctt cgtctcatgc tcgagtatat cgctgacaat gaacgcttgc
      421 cgttcaaaca gacactcctg agtgatgaag atgctgaact tgtggagata gtgaaagaac
      481 ggcttcgtaa tcctaagcca gtacgtgtga cgctggatga actctgatgg cgtattttct
      541 ggattttgac gagcgggcac taaaggaatg gcgaaagctg ggctcgacgg tacgtgaaca
      601 gttgaaaaag aagctggttg aagtacttga gtcaccccgg attgaagcaa acaagctccg
      661 tggtatgcct gattgttaca agattaagct ccggtcttca ggctatcgcc ttgtatacca
      721 ggttatagac gagaaagttg tcgttttcgt gatttctgtt gggaaaagag aacgctcgga
      781 agtatatagc gaggcggtca aacgcattct ctgaaccaaa gcatgacatc tctgtttcgc
      841 accgaaggtg acacttctgc tttgcgttga caggagaagc aggctatgaa gcagcaaaag
      901 gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca tagtgacggc actggtaacg
      961 aggaaagacc tctgcgaggt acgaatccga accggccaga cggaggtcgc tgtcttcaca
     1021 gcttacgaac ctgaggagta agagacccgg cgagtgagaa atccctcgcc acctctgatg
     1081 tggcaggcat cctcaacgca cccgcactta acccgcttcg gcgggttttt gtttttattt
     1141 tcaacgcatt tgaagttctg gatggtgccg gaatagaatc aaaaatactt aagtagcgcg
     1201 cagggataaa agggatggac cccgaacagg ggagtgctac ttatctggaa ggattctgat
     1261 gatgaacatc gaagaactgc gtaaaatttt ttgtgaagat ggcctctatg ctgtgtgcgt
     1321 tgaaaatgga aatattgtta gtcattaccg cattgtgtgt ttgcaaaaaa atggggctgc
     1381 gttaattaat tttgtggatg cccgagtgac ggacggattt atcttgcgcg acggtgagtt
     1441 tgtcgcttca ttacaggcat tgaaagagat cggaataaaa gctggctttt ctgctttttc
     1501 agaagaataa actcatctac aatcttgcgc ggggctgaac tcccgctgag taacaccgtg
     1561 ccaccggaga aaaccgatgg cacgcaacgc aaaatattac aatcatgata attcgaccgt
     1621 tcttgcccac acgcacgagc ggtattctca cgcatttaag tcagactggt accagcatcc
     1681 ctcatgcact gaagaacagg ccgaatggct cattcagtgt taccgcaggc gcggatgcga
     1741 ggttaaaaaa gcccttagcc tcgactaccg tcactggata atctccgtca ggctccctta
     1801 ctccgaacag ccaccgcgtc cgtcccgcac attccagcaa cggatctgga ggtaacgtgc
     1861 gggtattact tcgacctgtt ctggttccgg aactcgggct ggtggtcctt aagcccggtc
     1921 gtgaatccat gccggtattc cacaataccc ggttactggt ggagccggaa ccgaaaagca
     1981 tgcgtaatct gccgtccggg gtcgttcctg ccgctcgcca gccgctggtg gaagacaaaa
     2041 cattgctgct gtttttcagt aacgcacggg tgattcgtgc tgctggtggt gctggtgcat
     2101 tgtctgactg gctgttgcgc catattaaat cctgccagtg gccacacggc gattatcatc
     2161 acagcgaaac cgtcattcac cgttatggta ccggcgcaat ggtgttgtgc tggcactgcg
     2221 acaaccagct gcgtgaccag acatccgaat cactcgagca acttgctcat caaaacctgt
     2281 cagcatggat gattgacgtc atcggtcacg caataagcgg tacgcaggag cgtgaattat
     2341 ctctagctga attatcctgg tgggcggtcc gcaatcaggt ggcggacgcg ctaccggaag
     2401 cggtattacg tcgttcgctg gggttgcgtg cggaaaaaat ccgctcaatg taccgtgaaa
     2461 gcgacatcgt accgggagag cagaccgcca ccagcatact gaaacagcgc acaaaaaatc
     2521 ttgcgccgct gcctcacgcc caccagcaac agaacccacc acaggaaaag acggtggtca
     2581 gcattgccgt tgatcctgag tctccggaat ctttcatgaa acgacctaaa cgtcgccgct
     2641 gggttaacga gaaatacaaa cgctgggtga agacacagcc gtgtgcgtgt tgtggtaagc
     2701 cagccgacga tccccatcac ctgattggtc atggtcaggg cggaatgggg acaaaatctc
     2761 acgatatttt cacgctaccg ctgtgtcggg agcatcacaa cgagcttcat gcggatcctc
     2821 tggcgttcga agaaaagcat ggttctcagg ttgatttaat ttttcgtttt cttgatcacg
     2881 cctttgcaac tggcgtgctt gggtaaaaga ggtgactgat gctcatagat ttggttttac
     2941 cttacccgcc gacggtgaac acttactggc gacgccgtgg cagcacatat tttatctcgg
     3001 aggagggaaa gcgttatcgc cgggctgtgg cgcttattgt tcgccagcag cggctgaaat
     3061 taagcctgtc cggaaggctg gcgataaagg tgattgcaga gccaccggat aagcgtcgtc
     3121 gcgacctgga caatatcctg aaagcaccgc tggatgcgct gacgcatgcg ggagtgttaa
     3181 tggacgatga gcagtttgat gaaatcaata tcgttcgtgg tcagccagta tctggtggac
     3241 gtctgggggt gaagatttac ccca
//
LOCUS       DADLSB010000278         3244 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.279, whole genome shotgun sequence.
ACCESSION   DADLSB010000278 DADLSB010000000
VERSION     DADLSB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.279"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(152..313)
                     /locus_tag="JN331_004154"
     CDS             complement(152..313)
                     /locus_tag="JN331_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262099.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(310..513)
                     /locus_tag="JN331_004155"
     CDS             complement(310..513)
                     /locus_tag="JN331_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY7262100.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYPLMQAIVAEGDVVVSGATEPE"
     gene            758..1093
                     /gene="iraM"
                     /locus_tag="JN331_004156"
     CDS             758..1093
                     /gene="iraM"
                     /locus_tag="JN331_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7262101.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1374..1505)
                     /locus_tag="JN331_004157"
     CDS             complement(1374..1505)
                     /locus_tag="JN331_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY7262102.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2005..2081)
                     /locus_tag="JN331_004158"
     tRNA            complement(2005..2081)
                     /locus_tag="JN331_004158"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2045..2047),aa:Arg,seq:tct)
     gene            complement(2182..2257)
                     /locus_tag="JN331_004159"
     tRNA            complement(2182..2257)
                     /locus_tag="JN331_004159"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2222..2224),aa:Ile,seq:cat)
     gene            complement(2401..3222)
                     /locus_tag="JN331_004160"
     CDS             complement(2401..3222)
                     /locus_tag="JN331_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7262103.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          943 a          862 c          593 g          846 t
ORIGIN      
        1 ataccccgtc tcccgttatc cggaagctga caacaataaa aaagccacca gttaactact
       61 gatggctctg ataactcatg caagcgtctc agacgaccca ctgacactac cggtgagttt
      121 aacgatacct tccatttgac tggctcactt tttatgatga tgccggtgca tttatctcca
      181 gcaccagact ttctatctca acgccatacg ttgcattttt ggtaatatcc gtcagcgtca
      241 gtgcatttag tcccactgcc agactgtctt ttatggcctg gaatgccggg ccagtacgat
      301 gacgtagtat cactccggct cagttgcacc actgaccacc acatcacctt ctgctacaat
      361 cgcctgcatc aggggataag gggttatggc caccggacta ccaaacggct gccagccctc
      421 tttcagttta tgtgtcagct tttccgcaag atctgacggc ggcgccgccc tgacaacatc
      481 atagtgttta aatgccatgg ttctttccac catctgaaaa ataattcttt aaaatacctg
      541 acatgtaata cagaaaaaac acaaaaccat accttaaata aaaacctgat tatcaagcag
      601 atatgcatgg ataaactaca agacgagata taaaccaccc tgcatttaaa taaacaataa
      661 acaacatcag aaaaataatt ctgctctatg gtttaattca aaaatatcat ttattctttt
      721 cagaacatca ccagcaaggc ataaacaagg aaactaaatg aagtggattg tgattgatac
      781 agttatccag ccatcatgcg gaatatcttt ttcagtcata tggagtaaaa taaaattaat
      841 aatctggtat caatcggatg ctttcttacc tcctgaaagt atatttacac tgactcacac
      901 aggcatcatg ctcaataaca aagtgctacc tgtaaccatt tacaacgtag taccattcaa
      961 taaaacattc tggaatttaa tcaaaaacag ccaggaatgc cctacaaata cagataacgt
     1021 attgaatgaa tgctttaata accgttgcac tctgcaaata tgtccttatg gactaaaaca
     1081 acaaagtcca taaggagttt actcacatct gacaaaatca atataaacag cccctccgga
     1141 gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc ccggtgaatt
     1201 cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt gcccctccgc
     1261 acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca gacaacagtc
     1321 aaccgcctga attgtgaagt atttaaaaat ttctcccgct aactgatacc cggctaacag
     1381 tctggcgttt tctttttcag caacgggaaa gcaacaacca ccacacccgc caccagcaca
     1441 ccgtcagccg gcactgacat tatccggctg ctgcaatgcc attcacaaaa acagtaagca
     1501 atcacttttt accgtaacag gtgataatcc agatatgtat ctaccccaga tgagtaatcc
     1561 gaagttcatc cataccacag gtcctggcta ttctgttgta ctcctgaaca agagcaaata
     1621 attctgaatt agcaaccatg aactcatcgc aaaccctctg tatagcatca ctattcagaa
     1681 taataacgtc tcttcccgaa agacgatcag gagtacagaa caaaactgtc aaacggctga
     1741 aggcctttgc tcgtgctgca ttgactatat caatacgctg cctaaggatg aaacaccccg
     1801 acgcctcatc aatattcact ctacccacac catatgaatg ataaatactt aatgctgaaa
     1861 aaaccattag accgtataac aaatactcaa tcaatactta acagaacttt tatttttgac
     1921 aaacatataa tattttcaac aatatcctga gccaggtata tttcagtata aggctctgcc
     1981 ggaaggaatc tggaagaatg aatatggcgc gctgtactgg attcgaacca gtgaccgatt
     2041 gcttagaagg caattgccct gtccggctga gctaacaacg ctgaataccg ataatggacc
     2101 gccatcgggg acccgccccc gcaccaacaa ccctgttatc gtgtcgtctg ctcttcctga
     2161 taagctaatg gcggtttgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat
     2221 tatgagtcgc tcgctctcac cactgagcta aagggccggg agcagaataa taacggtccg
     2281 taattaattt cgcaataaaa aacccgctca gcggcgggtt gtagaaactc ttctaacgtc
     2341 aggcataaaa cgcccatcat tatgacgaat ttaccacaga ttccggaaaa atcaaccttg
     2401 ttacctagtt acctttttta actgccgctc agcccatgct tcttcaatat caaaccgggt
     2461 caccagcgca tcataaaatt tcttaactgt tttttcccat gacgcgcgtg ttatctggtt
     2521 tgtcacctcg catatagcat taaatgcctc cgttgatggt agtctttcat agccacgacc
     2581 accacaacgc tggcagtctc tgataacagg cataccacgt tttaccgact cttcacgatg
     2641 aatggcgaca ccacgcccac ggcaatcctt acaggcggtg gaaacctcac cctttccgcc
     2701 acactccgga caggcaactt ttaccacctc cctgactttt ttccattctt cccagtaaga
     2761 cggatacaca cctttcgtac actttgccca taccggcggc ttaccatccg gatactggat
     2821 cttatttgta aaaactatgc tttcaataaa tttttcccca tagcaacaag ggcactgctt
     2881 tttactcgct gcgctgcggg cataatcctc aaaagcgtac gaagccataa tgcgcatcac
     2941 taccggtttt atttctgccg gaagttttct caacgccgcc acacgatcgc accgactgag
     3001 tgcataatct gccagtaatt ctgttgcccg cgccctgtca ttcatactga tgcccatttt
     3061 ccccaggaac gcagaaaaac ccatctcagc ccgattctgt gtcatgccct gcgcggccat
     3121 cacatcagtg atactcagcg catcttttga cgttgaggcc gatgcatcgg tcaggccagg
     3181 ggattttggg gagtagtatt tcggtaaatc ttccagtttc attttttgac ctgctcttaa
     3241 tgca
//
LOCUS       DADLSB010000279         3218 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.280, whole genome shotgun sequence.
ACCESSION   DADLSB010000279 DADLSB010000000
VERSION     DADLSB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.280"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            80..343
                     /locus_tag="JN331_004161"
                     /pseudo
     CDS             80..343
                     /locus_tag="JN331_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            970..1515
                     /gene="cobU"
                     /locus_tag="JN331_004162"
     CDS             970..1515
                     /gene="cobU"
                     /locus_tag="JN331_004162"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY7262104.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDDGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            1512..2255
                     /gene="cobS"
                     /locus_tag="JN331_004163"
     CDS             1512..2255
                     /gene="cobS"
                     /locus_tag="JN331_004163"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY7262105.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            2267..>3218
                     /gene="cobT"
                     /locus_tag="JN331_004164"
     CDS             2267..>3218
                     /gene="cobT"
                     /locus_tag="JN331_004164"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY7262106.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQVGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLG"
BASE COUNT          758 a          683 c          903 g          874 t
ORIGIN      
        1 acaactagtg atagttgact tattttcgct cttgagagac aaccatactc aaatctccca
       61 cattgcagga gatttgaata tgaaaacgtc accgtggaat aaagaccgta ccatcggtca
      121 aaaaaggcca cttcagatat ctcatatctg ggggatccgg attagacttg agttggaagg
      181 taaaacacgc gatctagctc tgttcaacat ggctttggac agtaagctac ggggctgtga
      241 tttggtcaaa ctcaaagtat cagatgctgc atatggcagt tctgtctcaa gcagagcaac
      301 ggtgttgcaa cagaaaaccg gcagccctgt ccaatttgag atgcctttaa taacacatct
      361 tttaaatgtt gaagaattat cccgcctgct gaagaacgtt gctctgagtg tgataaacct
      421 ttttattctt gatgatgtca gcgagaagat ggaatatatc cccgactggg tcgtggttac
      481 ccggttaaac atctggtggc gctaataaat ctggcaagtc acattctgtt gaatgctgtg
      541 acagtgcctt accgagagag cgaaattgtg gtgttctagt gcatagtcgc caccattccg
      601 gataattcta ttgcgttgtt ttacaagttc ttttacagcg cagatctgtc gctgacgttg
      661 aatcaatagc ctggatttca gaacacaatg cccgtttgct ggttggtccg gcagtagcat
      721 taattcatta tctgaatgaa atgtgtcaat ccacagaaac cgctgtcaat gacgcatttt
      781 gtcggacgtt aacacgagaa agtctggcta ttccgtaatg ccatcttatt ttgtaatgga
      841 ttgagaatgc tggattttta attatattat gcgcagtatt aatcgcgtaa atgttttgcg
      901 gtcagcaacg gtgggggatc cattgctctg ttcaatgaat accatcatac atttgaatca
      961 ggagtcggta tgatgatttt ggtgacggga ggcgcacgga gcgggaagag tcgccacgca
     1021 gaggtgctta ttggggactc ttcacaggtt ctgtatatcg ctacctcgca aatccttgat
     1081 gatgagatgg ctgcacggat agaacatcat cggcaaagcc gcccggagca ctggcgcaca
     1141 gtggagcgct ggcaacatct tgatgaatta attcatgcag acattaaccc gaatgaggct
     1201 gtgttgctgg aatgcgttac cacaatggtg actaatctgt tgtttgatga tggcggcgat
     1261 aaagaccctg atgaatggga ttatcaggcg atggaacagg cgattaatgc tgagattcag
     1321 tcgttgattg ctgcctgcca acgttgcccc gcaaaggttg tattagtgac taacgaagtg
     1381 ggaatgggga ttgtgccgga gagtcgtctg gcacgacatt ttcgtgatat tgccgggcgg
     1441 gtaaatcagc agttggccgc tgcggcaaat gaagtatggc tggtggtttc gggtattgga
     1501 gtaaaaatca aatgagcaaa ttattttggg cgatgctctc atttattacg cgtctgcctg
     1561 taccgcgtcg ctggtcccag ggactggatt tcgagcatta ttctcgcggt attattactt
     1621 ttcctttgat tggattattg cttggcgcga ttagcgggct ggtcttcatg gtgctgcagg
     1681 catggtgtgg cgcaccactg gcggcactgt ttagcgtact tgtgctggcg ctgatgaccg
     1741 gggaattcca tctggacggc ctcgctgata cctgtgatgg cgtattttct gcacgtagcc
     1801 gcgatcgcat gttggaaatc atgcgtgata gccgtttagg cacccacggc ggtctggcat
     1861 tgatttttgt ggtactggca aagattctgg tgttaagcga gttggctctg cgtggcgaac
     1921 cgatccttgc atcgctggcg gcagcatgtg cggtcagccg cgggactgcc gcattattga
     1981 tgtatcgtca tcgttatgcg cgggaagaag ggcttggcaa tgtgttcatt ggcaaaattg
     2041 atgggcgaca aacctgcgtc acactcggct tagccgctat ttttgctgcc gtattattgc
     2101 cgggtatgca tggtgtggct gctatggtgg tgacgatggt ggcgattttc atcctcggtc
     2161 agcttctcaa acgtacgctg ggcgggcaaa cgggcgatac gctgggagca gccatagagc
     2221 ttggtgaact ggtcttcctg ctggcactgc tataaagaga cgtgacatgc aaataattgc
     2281 cgatttactg aatacgatcc cagccatcaa ttctgccgct atgtcgcgtg cacaacggca
     2341 tgttgacggg ttactcaaac ctgttggtag cctaggaaag ctggaggcgc ttgccataca
     2401 actggcagga atgccggggg tgaatggcat accgcatgtg ggcaaaaaag cggtactggt
     2461 tatgtgtgcc gatcacggcg tctgggagga aggggtcgct atttctccaa aagaagtgac
     2521 agccatacag gctgaaaata tgacccgtgg aacaaccggc gtgtgtgtgc tggcagcaca
     2581 agtgggcgct aacgtccacg taattgatgt tggtattgat actgctgagc ctatccccgg
     2641 gcttatcaac atgcgtgtcg cacgaggtag cggcaatatt gcttcagctc cggcaatgag
     2701 tcgccgtcag gctgaaaagt tgcttttgga cgtcatatgt tatacgcggg agctggcaaa
     2761 aaacggtgtc acgctgtttg gtgtaggtga actggggatg gcaaacacga caccggcagc
     2821 ggcaatagtc agcacaatca ctggccggga tcctgaagaa gtggttggga ttggtgcaaa
     2881 cctgccgaca gataaactgg ctaataaaat tgatgttgtg cgtcgggcga ttacgttgaa
     2941 tcaaccaaat cctcaggatg gtgttgatgt cctggcaaaa gtgggtggat ttgatttggt
     3001 cggaatagct ggagtgatgt taggtgctgc ttcctgcggt ttacccgtgt tgctggatgg
     3061 atttctttct tatgctgctg cgctcgcagc ctgccagatg tctcctgcaa tcaaaccgta
     3121 tctcattcct tctcacttgt cggcagaaaa aggcgcgcgt atagcgctct cgcatttggg
     3181 gctggagcct tatctcaata tggatatgcg tttaggtg
//
LOCUS       DADLSB010000280         3217 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.281, whole genome shotgun sequence.
ACCESSION   DADLSB010000280 DADLSB010000000
VERSION     DADLSB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.281"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1166
                     /locus_tag="JN331_004165"
     CDS             <1..1166
                     /locus_tag="JN331_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY7262107.1"
                     /translation="IGGDKSIPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILR
                     AASFGNAQLMIPMVHSLDQILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCY
                     IIDHFCDEVDFFSIGSNDMTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQR
                     GKWVGICGELGGESRYLPLLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQAC
                     ECRSAQEIEALLTAFTPEEDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEH
                     PFELEEDVWQREEIVTTGVGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVEL
                     VIMLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(1169..2026)
                     /locus_tag="JN331_004166"
     CDS             complement(1169..2026)
                     /locus_tag="JN331_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7262108.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2039..2773)
                     /locus_tag="JN331_004167"
     CDS             complement(2039..2773)
                     /locus_tag="JN331_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7262109.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(2788..>3217)
                     /locus_tag="JN331_004168"
     CDS             complement(2788..>3217)
                     /locus_tag="JN331_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY7262110.1"
                     /translation="KDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAIVH
                     GIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNVHH
                     RVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT          780 a          837 c          839 g          761 t
ORIGIN      
        1 acatcggcgg cgataaaagc attccttatc tgaatattcc ccaggaagag aacccgttcc
       61 tcggctatcg cgcggtacgt atttacccgg aatttgctgg cctgttccgc actcaactgc
      121 gggccatttt gcgcgccgcc agtttcggca acgcccagtt gatgatcccg atggttcaca
      181 gcctcgatca gatcttatgg gtgaaaggcg agatccaaaa agcgatcgtt gagcttaagc
      241 gcgatggcct gcgtcatgca gagacgatta cgcttgggat catggtggaa gtgccgtcgg
      301 tgtgctacat catcgaccac ttctgcgatg aggtcgattt cttcagtatc ggctccaacg
      361 atatgaccca gtatctgtat gcggtcgatc gtaataaccc gcgcgtatcg ccgctatata
      421 acccgattac gccatcgttc ctgcgcatgt tgcagcaaat agttaccact gcgcatcagc
      481 ggggcaaatg ggtaggcatt tgcggtgaac tgggcggtga aagccgttat ctgccgctac
      541 tgcttgggct gggcctggat gagctgagta tgagtagccc gcgtattccg gcggtgaaaa
      601 gccagcttcg tcaactggat agcgaggcgt gtcgggaact ggcgcgtcag gcatgtgaat
      661 gccgcagtgc gcaggaaatt gaagcgctac tcaccgcctt tacgccggaa gaagacgttc
      721 gcccactgct ggcgctggag aatatctttg ttgatcagga ttttagcaat aaagagcagg
      781 cgatccagtt cctgtgcggc aacctcggcg ttaacgggcg cactgaacat ccgtttgagc
      841 tggaagaaga tgtctggcag cgggaagaga ttgttaccac cggcgttggt tttggcgtag
      901 cgatcccgca caccaaatct cagtggatcc gtcattccag tatcagcatt gcccggctgg
      961 cgaaaccggt tgactggcag tcagaaatgg gcgaagtcga actggtgatc atgctgacgc
     1021 tgggtgctaa cgaagggatg aatcatgtga aagtcttctc gcagctggcg cgtaaactgg
     1081 tgaataaaaa cttccgccag tcgttgtttg ccgcgcaaga tgcacaaagt atcctgacgc
     1141 tgctggaaac agaattaacc ttctgacgtt agccctgaaa acgggcgctg tactctcccg
     1201 gcgtcagacc aaactgacgc cggaaaacgc gacaaaaata gtcgctatcc ggaaaaccgc
     1261 aacgctgtgc cacttcatga attgacagat gatatttctg caaaatcatc ctcgccttcg
     1321 ccattcgcac ccaacgcaca tactcgataa aacgcatcgt tccatgctga gcaaacagtt
     1381 ttgacagatg attgggcgtg atattaaaaa actgcgccac gctctcgcgg gtgagcggct
     1441 gggcataatt atcctgaacc cagttacaaa tgctgtgata gagaaaatcg ccgcgcggtt
     1501 gcgaattacc cggcggcata ttcaccacgt tgcggcaaag atgcagcaaa cttaacacca
     1561 gtggctgaat gatattctgc tccagcggcg aacggctaag atgggtgagg gcggtaagca
     1621 tcgcttcacc ttcaccgcgt tgcaggctgg gaagctggat ctggcgagca ggatgcaaca
     1681 acgacgtggt gcggctatcg taaaacgata acccaagcca ggtcggtgcg aacaccaggc
     1741 ttaacagcat caccggtttg ttgttgatcg gtaaattagc cgcgcgtgcc ggaataaaca
     1801 gcatttctcc ttccaccatt cgctgctgct ttgcttccag caggttgccg tattcgccgc
     1861 gcagcacgat atccagacgg ggaaaatcaa tacataaact gcccaccggt aacgaggcag
     1921 gctggctggc aaaccagacg cgccccagta gttgcgggtt aagcaccagg ccgctgaaca
     1981 gatcggcgaa aaattgttga tcggcgggca agcctggtgc tttcatgaaa gttcccgatt
     2041 aaagatgcat taactggcga aattctttca ctttgctgcg gctgaccggc acttcaaaat
     2101 ccagatcttt cagtcgcaga atgtaggtgt tattaaacca cggttcgatt tcgcgtattt
     2161 tgttcagatt gacacaaaat gagcgatggc agcggaaaaa atgcgacggc ggcagtttgc
     2221 tgcaaaattc ggtaatgttc atcggcatta cgtaggattc acgccgcgta tagacaaagg
     2281 tcattttctc gtgcgcttcg gcgtaataga tatcgttaat tggcgtgacg attattcgct
     2341 catctttcac cagattaatg gtgtcattct cacgcgttac cgtcgcggca ggcgtactgc
     2401 tggtctgctg ttgttgccag gccgcttcca gtttttgcag catcccggta atacgtgact
     2461 cctgatacgg tttgagaatg tagtcaaacg cctccagttc aaacgcttct accgcatgtt
     2521 ctttccacgc ggtgatgaac acaataaacg gtttatgggc gaactggctg atgttttgcg
     2581 ccagcaacac gccatccagc gacggaatat tgatatccag aaaaatggcg tcgacgcggt
     2641 tatgctgcaa aaacttcaac acgtccagac cgtcgtcaaa ggtgccgaca atctccatct
     2701 ggctgtgctc tttaattagc cagctcagtt cctgttgtgc caggaattcg tcttcaacaa
     2761 tgatgacttt cacaatatca ctccggctca aagcaataac gtagcctgtg aggcgactgg
     2821 ggtgcgttgg ttaggaatgt aaaacgcaat ttccgtcccc ggctccaggc ggcggatatg
     2881 cagcccctcg ccatacaata acttcacgcg atgatggaca ttcagcaggc caattttatt
     2941 gcccggcatt tcattcgctt cgacccgctc aatcaccttt ggatcgatgc cgtgcccggt
     3001 atctcgcacc gcaatgcgta cccgatttcc gcactctgca acgctgatgg tgacaacgcc
     3061 tttacctttg caaggctgaa taccgtggac aatggcgttc tccaccaacg gctggatcag
     3121 caggctggga atgcagcaat tcacctcttc atcaatatca tagataaccg tcagcttgtc
     3181 gccaaagcgg gcctgctcaa tggcaatata gtcttta
//
LOCUS       DADLSB010000281         3197 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.282, whole genome shotgun sequence.
ACCESSION   DADLSB010000281 DADLSB010000000
VERSION     DADLSB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.282"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            20..610
                     /locus_tag="JN331_004169"
     CDS             20..610
                     /locus_tag="JN331_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY7262111.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            850..1110
                     /locus_tag="JN331_004170"
     CDS             850..1110
                     /locus_tag="JN331_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY7262112.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1334..1408
                     /locus_tag="JN331_004171"
     CDS             1334..1408
                     /locus_tag="JN331_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY7262113.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1401..2258
                     /gene="repA"
                     /locus_tag="JN331_004172"
     CDS             1401..2258
                     /gene="repA"
                     /locus_tag="JN331_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY7262114.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2958..3098
                     /locus_tag="JN331_004173"
     CDS             2958..3098
                     /locus_tag="JN331_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262115.1"
                     /translation="MAENGYGLAGLGVGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CR"
BASE COUNT          824 a          802 c          823 g          748 t
ORIGIN      
        1 tgccaggagc agataaataa tgaagttgat tatttttatt ctcattgttc tgattattgc
       61 agcgctactt atccggatta tcctcagaag tgtaaatcag cattctcctt tgctgatgca
      121 attgcatgcg gcaggtatac ggaccggtga tgcagagcga atactgtcca gcggtgaata
      181 ctggcaacgt cagaagacgc tgctgacaga aagggaagtc agttttatga aaggactgtt
      241 cagaattgtg gatatgaagc ggtggtatct gtgtccgcag gtacgggtcg cggatatcgt
      301 ccagctgaac gggaatatcc ggccacgatc acgccagtgg tggcagttat tcaggatggt
      361 gtctcagtgg catgttgatg tggttatcgt tgagctgcgt tcgttcagta ttgttgcagc
      421 agtagagctg gatgatgcca gccatttacg accggaacgc agacgccggg atattcttct
      481 ggaagaggtt ctgaggcagg ctggtattcc gttgctcaga agccacgatg ccagaaaact
      541 gctgcagatg accggagaat ggctgaatac aacaggggct gatcagcagt caccggaaca
      601 tcgtagctga cgccttcgcg ttgctcagtt gtccaacccc ggaaacggga aaaagcaagt
      661 tttcctcgct cccggcgttt ccataactga aaaccatact atttcacagt ttaaatcaca
      721 ttaaacgaca gtaatccccg ttgatttgtg cgccaacaca gatcttcgcc acaattctca
      781 agtcgctgat ttcaaaaaac tgtagtatcc tctgcgaaac gatccctgtt tgagtattga
      841 ggaggcgaga tgtcgcagac agaaaatgca gtgacttcct cattgagtca aaagcggttt
      901 gtgcgcagag gtaagcctat gactgactct gagaaacaaa tggccgctgt tgcaagaaaa
      961 cgtcttacac acaaagagat aaaagttttt gtcaaaaatc ctctgaaaga tctcatggtt
     1021 gagtactgcg agagagaggg gataacacag gctcagttcg ttgagaaaat catcaaagat
     1081 gaactgcaga gactggatat actaaagtaa agactttact ttgtggcgta gcatgctaga
     1141 ttactgatcg tttaaggaat tttatggctg gccacgccat aaggtggcag ggaactggtt
     1201 ctgatgtgga tgtacaggag ccagaaaagc aaaaaccccg ataatcttct ttaactttgg
     1261 cgagtcagaa agattaccgg ggcccactta aaccgtatag ccaacaatca agctatgcgg
     1321 ggagtatagt tatatgcccg gaaaagttca agacttcttt ctgtgctcgc tccttctgcg
     1381 cattgtaagt gcaggatggt gtgactgatc ttcaacaaac ttattaccgc caggtaaaga
     1441 acccgaatcc ggtgttcaca ccccgtgaag gtgcccggac gcttccgttc tgcgggaaac
     1501 tgatggaaaa ggcggtgggc ttcacctccc gttttgattt cgccattcat gtggcacatg
     1561 cccgttcgct tggtctgcgt cggcgcatgc caccggtact acgtcgccgg gccattgatg
     1621 cgctgctgca ggggctgtgt tttcactatg acccactggc caaccgcgtc cagtgttcca
     1681 tcaccacgct ggccattgag tgcggactgg cgacggagtc tgctgccgga aaactctcca
     1741 tcacccgggc cacccgggcc ctgacgttcc ttgcagagct gggactgatt acctaccaga
     1801 cggaatatga cccgcttatc gggtgctaca ttccgaccga tatcacgttc acatctgcac
     1861 tgtttgctgc cctcgatgta tcagaggagg cagtggccgc cgcgcgccgc agtcgtgtgg
     1921 aatgggaaaa cagacagcgc aaaaagcagg ggctggatac cctgggtatg gatgaactga
     1981 tggcgaaagc ctggcgtttt gttcgtgagc gtttccgcag ttaccagaca gagcttaagt
     2041 cccgtggaat gaagcgtgcc cgtgcgcgtc gtgatgcgga caggcagcgt caggatattg
     2101 tcaccctggt gaaacggcaa ctgacgcgtg aaatctcgga agggcgcttc actgccagtc
     2161 gtgaggcggt aaaacgtgaa gtggagcgtc gtgtgaagga gcgcatgatt ctgtcacgta
     2221 accgtaatta cagccggctg gccacagctt ccccctgaaa gtgacctcct cagaataatc
     2281 cggcacgcgc cggaggcatt cgcacgcctg aagcccgcca gtgcacaaaa aatcagcgct
     2341 acatacaaaa aacaacctca tcatccacct tctggtgcat ccggtccccc ctgtttttga
     2401 tacaaaacac gcctcacaga cggggaattt tgcttatcca cattaaactg caagggactt
     2461 caccataagg ttacaaccgt tcacgtcata aagcgccagc cgtcagccct acagggtgcg
     2521 atgtatcttt taaacgcctg tttatatctc ctttaaacta cttaattaca ttcatttaaa
     2581 aaaaaaacct gttcactgcc tgtcctgtgg acagacagat atgcacctcc caccgcaagc
     2641 ggcgggcccc aaccgaagct ggtttagtta caacatgcaa acataaaccc ctaaaaaacc
     2701 caagtccgac gtagaactga aaccacaacg cccctcctca taactaaaaa gcgccgccgc
     2761 ccccgcccga agggcgggaa cagagtcgct tttaattatg aatgttgtaa ctacatcatc
     2821 atcgctgtca gtcttctggc tggacgtatc gagtacacgc tcgtaagcgg ccctcacggc
     2881 ccgctaacgc ggagatacgc cccgacttcg ggtaaaccct agtcgggacc actccgaccg
     2941 cgcacagaag ctctgtcatg gctgaaaacg ggtatggctt ggcagggctg ggggtgggta
     3001 aggtgaaatc tgtcaatcag taccggctga cgccgggctt cggcggtttt actccggtat
     3061 cacatgtaac cacagcgtgc cgccttccat gccgctgacg cggcatcaga ataatacaag
     3121 cagcatttaa tgcatttgca aaagtgtgag taataaagac ttgttaagtg gtcgtttcag
     3181 ggatatcgtt aagactt
//
LOCUS       DADLSB010000282         3181 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.283, whole genome shotgun sequence.
ACCESSION   DADLSB010000282 DADLSB010000000
VERSION     DADLSB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.283"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            171..278
                     /locus_tag="JN331_004174"
     CDS             171..278
                     /locus_tag="JN331_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY7262116.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(259..990)
                     /gene="yehW"
                     /locus_tag="JN331_004175"
     CDS             complement(259..990)
                     /gene="yehW"
                     /locus_tag="JN331_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY7262117.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQATLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(995..1921)
                     /gene="yehX"
                     /locus_tag="JN331_004176"
     CDS             complement(995..1921)
                     /gene="yehX"
                     /locus_tag="JN331_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAY7262118.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(1914..3071)
                     /gene="yehY"
                     /locus_tag="JN331_004177"
     CDS             complement(1914..3071)
                     /gene="yehY"
                     /locus_tag="JN331_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAY7262119.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3078..>3181)
                     /locus_tag="JN331_004178"
     CDS             complement(3078..>3181)
                     /locus_tag="JN331_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7262120.1"
                     /translation="AKTLQQLNASIAVEGLDAKKVAADYLKQKGWAK"
BASE COUNT          805 a          986 c          784 g          606 t
ORIGIN      
        1 ttcacagata aaacactctc caggaaaccc ggggcggttc actccgccca acagcagcaa
       61 ctcaccagtt ggcaagaaca aggccatgat attttcccac tcggtattta atgattcgtt
      121 aacaaatgcg ctttactgta caatcctttc gttaacataa ggagtgcatt atgcgcatag
      181 ctaaaattgg ggtcatcgcc ctgttcctgt ttatggcgtt aggcggaatt ggtggcgtca
      241 tgctcgcagg ttataccttt attttgcgtg ctggctaagc gcctgcacca gcctttcaaa
      301 caggcggtct gcgatgatcg ccgccagtgc caccagtaac gccccctgga tcacatacgc
      361 ggtattaaat ccgctaagcc cgatgatgat gggcgtaccc agcgtactgg cccctaccgt
      421 tgaggcgatc gtcgccgtac caatgttgat aatcaccgaa gttcgcacgc ccgccagaat
      481 caccggagcc gccagcggta gctcgacctt acgcagtcgc tgacctcgac tcattcccat
      541 acctttcgca acttctgtca cgctgggatc aatcgctccc agcccggcaa gtgtcgcctg
      601 caggatgggc agcacaccgt aaaggatcaa ggcgataatc gctggttgca gaccaaagcc
      661 gatcaccgga acggcaatcg ccagcactgc gacaggcgga aaagtctgtc caacggcggc
      721 aatagtttcc accagtgggc gaaattccgc gccccacggg cgagtaacag caattccggc
      781 acctgtgcca atgatcaccg caaacaaact cgaaattccc accagccaga aatgagccag
      841 tgccagagct gcaaaacttt cttgctgata aacgggtcgt ggcagttgtg ggaacaaggc
      901 agcaaacagc ggctggctgt aaggcagcca gaaaatcagc gccacaaaaa gagcaatgag
      961 ccagaacagc ggatcgcgca acatcttcat acgcttacgc ctccaccagc agatcctgaa
     1021 aatgcagcgt gccgcaaggc tggccctgcg tgttcaccac cggcagcacc tcgcatcccc
     1081 gcgcgacaaa cagggagagc gcatcgcgta gcgtcatctc ttctgccagt gcctcacctt
     1141 ctgcccgttc ttcgcgacgc acgtaatccg ccacgctacg taacgaaagc aggcgcaccc
     1201 ccagttcact acgtccaaaa aactggcgga caaaatcatt cgccggacga gtcagcatcg
     1261 tcagcggatt cccctgctgc accacttcac cgtgatccat caataccaga tgttctgcca
     1321 gccgtagcgc ctcatcaata tcatgagtga ccagcacaat agtgcgcccc agcaaacggt
     1381 gaatgcgcgt catctcttgt tgcaacgcgc cgcgcgttac cgggtccagt gcgccaaaag
     1441 gttcatccat cagtaagact tgcggatcgg cagccagtgc acgcgccact cccacacgtt
     1501 gctgctgacc accggaaagc tgatgcggat aacgctcacg caaatttgac tccagcccca
     1561 gtagcgccat taattcgtcg atacgatcgt caatccgcgc ccgcgaccat ttttgtaatt
     1621 gcggcacggt agcgatgttt tgcgccacgc tccagtgtgg gaacaggcca atagattgaa
     1681 tggcatagcc catccggcgg cgcaactcca gtactggcag cgagcgaatt tcttctccgg
     1741 caaagcggat cactccgctg tcatgctcca ccaggcggtt aatcattttc agggtggtgg
     1801 atttgccgga gccagatgtg ccaatcagca ccgaaaaact cccttcctga aaattgagat
     1861 tgagatcgtt aacggctttt tgtgcgccga acagtttgct gacatggcta aattcaatca
     1921 ttacgtttca ccttcagcag tgcgataagc aaatcgaaca gcgcgtcggt aagcaccgcc
     1981 agaacaatta ccgggatcac ccccagcaac actaaatcaa tggcgctgct tagcagcccc
     2041 tggaaaacca gcgcaccaaa accgcctgcg ccgattaacg ccgcaatcac cgccatacct
     2101 acagtttgca ccatcaccac ccgcaggctg cgcagaaata ccggtaacgc cagcggtaac
     2161 tgaacatgca ggaaacgctg cgccccgctc atccccatcg ctctggcgct ctccagcaca
     2221 tcgcgcggga tctggttcaa accgactacc acgccgcgca ccagcggcag caaggcatag
     2281 agcaccagcg caatcagtgc gggtgtcatt ccggttcctg ctatgccgag cttccccagc
     2341 cacggaaagg ccgtaaccag cgcggcaagc ggcgcaatca acaggccaaa gagcgccacc
     2401 gaaggcacgg tctgaatgac attgagcaga gaaaaaattg ccccctgccg cgcagtggaa
     2461 aagtagcacc agatgcccaa cggcacacca atcactaacg caggcagcac cgcaccaaac
     2521 agcaacgtca gatgttgtgc cagcgcgtcg tcaaacacat cctgacggtt ggcgtattct
     2581 ttcattagtg agagatcgtt aagcgtgccg gagtacagca accacagcgg aataatggca
     2641 atctgcatat gcaacaacca gcgccacagc ggatgcgtgg agattcggcg gatggcatcg
     2701 ctacaggcca gcaatgccag cgccgcagcc agccagaaac cactgccgag gctggtacgc
     2761 gccagcgcac tgccattttg cgccagttgg gtcgccgcct ttccagctcc ccacaccaac
     2821 aatacgaaga cgaattgcgc cagaatgagt gcaaaaatgc tgcctttttt agcggggaca
     2881 aaacaggctg tcagccaggc gcaaccaacg cccaccagca tccagatcgt ttgcggccac
     2941 agttgccaga gatgacgccc ttcacccgag actaaacgat taggcgcgta actgataaac
     3001 ggcagcgccg ctgcgattgc cgtcagcaac aacagcagcg ccagaacagg attaatacgt
     3061 agataagtca cgggaaatta cttcgcccac cctttttgtt tcaggtagtc ggcagccact
     3121 tttttggcat ccagtccttc cactgcaatg ctggcattca gttgctgcaa tgtttttgca
     3181 t
//
LOCUS       DADLSB010000283         3124 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.284, whole genome shotgun sequence.
ACCESSION   DADLSB010000283 DADLSB010000000
VERSION     DADLSB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.284"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            52..771
                     /locus_tag="JN331_004179"
     CDS             52..771
                     /locus_tag="JN331_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY7262121.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCIYAVKSYPTYLADEPEWASRAE
                     KVAVRMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            782..2209
                     /locus_tag="JN331_004180"
     CDS             782..2209
                     /locus_tag="JN331_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY7262122.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2202..2897
                     /locus_tag="JN331_004181"
     CDS             2202..2897
                     /locus_tag="JN331_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY7262123.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
BASE COUNT          799 a          675 c          890 g          760 t
ORIGIN      
        1 tgggggggaa acatcgggca taaatgggca tgaagtaatg gagtattagt tgtgaatgtc
       61 aatttctttg tcacctgtat tggtgacgcc ctgaaatcaa gaatggcacg agactccgtg
      121 ctgctactgg aaaaactcgg ctgtcgcgta aatttcccgg agaaacaggg atgctgcggt
      181 cagcctgcga tcaatagcgg ttatatcaaa gaagcgattc cagggatgaa aaatctgatc
      241 gccgcactgg aggataacga cgatcccatt atttcaccgg ctggctcttg catctatgcc
      301 gtaaaaagtt acccgacgta tctggcggat gaacctgaat gggcatcacg tgccgaaaag
      361 gttgccgtgc gtatgcagga tctcacctct tttattgtta ataaattagg ggtagtcgat
      421 gtaggtgcca gtttgcaagg gagagctgtg tatcacccat cttgtagcct ggcccgtaag
      481 ctgggagtga aggacgagcc acttacgctg ctgaaaaatg tgcgtggact ggagctgttg
      541 acctttgctg aacaggatac ctgctgcgga tttggcggca cgttctcggt caaaatggcc
      601 gaaatatccg gcgagatggt gaaagaaaag gttgtgcacc tgatggaagt ccgccctgag
      661 tatttaattg gtgctgacgt gagttgcctg ctgaacatca gtgggcgatt acaacgggaa
      721 gggcagaaag tcaaagtgat gcatattgct gaagtgttga tgagccgctg aggatataaa
      781 gatgtcgatc aaaaccagta atacagattt taagacacgc atccgtcagc aaattgaaga
      841 tccgatcatg cgcaaagcgg tggcaaacgc gcagcagcgt attggggcaa atcggcaaaa
      901 aatggtcgat gaattggggc actgggagga gtggcgcgat cgggccgccc agatacgtga
      961 tcatgttctg agtaatctcg acgcttatct gtaccagctc tcagaaaaag tgacgcaaaa
     1021 cggcggtcac gtctattttg caaaaaccaa agaagacgct acccgctaca ttttacaggt
     1081 tgcccaacgc aaaaatgccc ggaaggtggt gaaatctaaa tcgatggtga ccgaagagat
     1141 tggtgtcaat catgtgttgc aggatgctgg cattcaggtg attgaaaccg atctgggtga
     1201 atatattctc cagctggatc aagatccgcc atctcatgtt gtggtcccgg caattcataa
     1261 agatcgccat cagatccgtc gagtgctaca cgaacgtctg ggctatgagg ggccggaaac
     1321 gcctgaagcg atgaccttat tcatccggca aaaaatccgt gaagatttcc tcagtgctga
     1381 aataggtatt accggctgta atttcgcggt ggcagagacc ggttcggtat gcctggtgac
     1441 caatgaaggt aatgcgcgaa tgtgtaccac gctgcctaaa acgcatattg cagtgatggg
     1501 aatggagcgt attgccccca cgtttgccga ggtagatgta ttgatcacca tgctggcgcg
     1561 cagtgccgtt ggtgcacgtt tgacgggata caacacctgg ctgacagggc cgcgcgaagc
     1621 ggggcacgtt gatggtcctg aagagtttca tctggttatt gtcgataacg ggcgttctga
     1681 ggtgctggcc tctgaatttc gggatgtgct gcgctgtatt cgctgcgggg cttgtatgaa
     1741 tacttgtccg gcatatcgcc atattggcgg tcatggatat ggctctattt atccagggcc
     1801 aattggtgcg gtgatttctc cgctacttgg cggctataaa gattttaaag atttacccta
     1861 cgcctgctct ttatgcactg cttgtgacag cgtgtgtccg gtgcgtattc cgctgtcaaa
     1921 actgattttg cgtcatcgtc gggtgatggc tgaaaaaggg atcaccgcaa aagcagagca
     1981 acgggcgata aaaatgttcg cttatgccaa tagtcatcca ggattgtgga aagtcgggat
     2041 gatggccggc gctcatgcgg caagctggtt tatcaatggc ggcaaaacac cactcaaatt
     2101 tggcgcgatt agcgactgga tggaagcacg cgatcttcct gaagctgacg gagagagttt
     2161 ccgtagttgg tttaagaaac atcaggcgca ggagaaaaag aatggataat cggagcgaat
     2221 ttttgaataa cgttgctcag gcactgggtc gcccgctgcg acttgaaccg caagcagaag
     2281 atgcgccgct taacaactat gctaacgagc ggcttaccca acttaaccaa cagcagcgct
     2341 gtgacgcgtt tattcagttt gccagcgatg ttatgttgac gcgctgtgag ctgaccagcg
     2401 aggcgaaggc ggcagaagct gcaatacgtc tgtgtaaaga gctgggagat cagtcggtcg
     2461 tgattagcgg tgacacgagg ctggaggaat tggggattag tgaacgtttg cagcaggaat
     2521 gcaatgccgt tgtttgggat ccggcgaaag gtgccgagaa tatctcgcag gcagagcagg
     2581 ctaaagtggg tgtcgtgtat gctgaatatg gtttaaccga atcgggaggc gtggttcttt
     2641 tttccgccgc cgagcgcggg cgttcattga gcctgctccc ggaatcttct ctttttatcc
     2701 tgcgtaaaag cacaatcctg ccgcgtgtag cgcaactcgc agaaaaattg catcagaaag
     2761 cgcaggccgg tgaacgaatg ccgtcatgta ttaacatcat cagtggtccc agctcaacgg
     2821 cggatattga gcttatcaaa gtcgtcgggg ttcatggtcc ggtgaaagcg gtgtatctga
     2881 ttattgagga ttgctgagag agatatgaga tctgaacata aacctgaaga aggcatacga
     2941 ggttgaaaat agcgttaagg acaacctgac ttaaccccgt tccgtcatgg ttatgctgct
     3001 cttctgtaaa tatcctggca ttagtaatgc caggatattt acagtgaaaa agacaggcat
     3061 ccattcctga aaacgggttc ccttcccggt cattgtcact tcagctttat acaggcactc
     3121 tcta
//
LOCUS       DADLSB010000284         3113 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.285, whole genome shotgun sequence.
ACCESSION   DADLSB010000284 DADLSB010000000
VERSION     DADLSB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.285"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..345
                     /locus_tag="JN331_004182"
     CDS             <1..345
                     /locus_tag="JN331_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7262124.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
     gene            335..1153
                     /locus_tag="JN331_004183"
     CDS             335..1153
                     /locus_tag="JN331_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7262125.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGANPRRRQWSITTDTGISYAT"
     gene            1143..2567
                     /gene="patD"
                     /locus_tag="JN331_004184"
                     /pseudo
     CDS             1143..2567
                     /gene="patD"
                     /locus_tag="JN331_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            2777..3113
                     /locus_tag="JN331_004185"
                     /pseudo
     CDS             2777..3113
                     /locus_tag="JN331_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
BASE COUNT          728 a          688 c          869 g          828 t
ORIGIN      
        1 atcctgcccg ttcaggcggc gcttgagcgt ttgccgccgt cattgttgca ggcgtcggct
       61 gatctcggcg cacgtccacg acaaaccttt cgctatgtgg tgctgccgct ggcaatcccg
      121 ggtattgccg ctggctctat ctttaccttc tcactcacac tgggcgattt tatcgtcccg
      181 cagctggttg gtcctccagg atattttatc ggcaatatgg tttattccca gcagggggcg
      241 attggcaata tgccgatggc ggcggcattc accctggtgc cgattattct catcgcactg
      301 tacctggcgt tcgtgaaacg tctgggagcg ttcgatgcac tctgaacgcg ctccgttttt
      361 cctcaaactg gcggcctggg gcggcgttgt tttcctacat tttcccatcc tgataatcgc
      421 cgcctatgcg tttaacactg aagatgcggc gtttagtttt ccaccgcagg gcctgacgct
      481 gcgctggttt agcgtggcag cacagcgtag tgatattctt gatgccgtga cgctgtcact
      541 taaagtggcg gcgctggcga cattaattgc gctggtgtta gggacgctgg cagctgccgc
      601 gctgtggaga cgagactttt tcggcaaaaa cgccatttcg ctgttattgc tgctgcccat
      661 tgcgctgccg ggcattgtca ctggtctggc gctattaacc gcctttaaaa ccatcaatct
      721 ggagccggga tttttcacca tcgtggtcgg tcatgcgact ttttgcgtag tggtggtgtt
      781 taacaatgtc atcgcccgtt ttcgccgcac ctcgtggagt ctggttgagg cgtcaatgga
      841 tcttggggcc aatggctggc aaaccttccg ctacgtagtg ttgccgaatc tcagttcggc
      901 gttactggca ggaggaatgc tggcgtttgc cttgtcgttc gatgaaatca tcgttacgac
      961 ctttacggca ggtcatgaac gaacgttacc gttgtggttg ctcaatcagc ttgggcgacc
     1021 gcgtgatgta ccggtaacca acgtggtggc actgctggtt atgttggtaa caaccttgcc
     1081 gatcctgggg gctaacccgc gaaggcgaca atggtcaata accactgata caggaataag
     1141 ctatgcaaca taagttactg attaactgag aactggttag cggcgaaggg gaaaaacagc
     1201 ctgtctataa tccggcaacg ggggacgttt tactggagat tgccgaggca tccgcagagc
     1261 aggtcgatgc tgctgtgcgt gcggcagatg cagcatttgc tgaatggggg caaaccacac
     1321 cgaaagcgcg tgcggaatgc ctgctgaaac tggcggatgt tatcgaagaa aatggtcagg
     1381 tttttgccga actggaatcc cgtaattgtg gcaaaccgct gcatagtgcg ttcaatgatg
     1441 aaatcccggc gattgtcgat gtttttcgct ttttcgcggg tgcggcgcgc tgtctgaatg
     1501 gtctggcggc aggtgaatat cttgaaggtc atacttcgat gatccgtcgc gatccgttgg
     1561 gggtcgtggc ttctatcgca ccgtggaatt atccgctgat gatggccgcg tggaaacttg
     1621 ctccggcgct ggcggcaggg aactgcgtag tgcttaaacc atcagaaatt accccgctga
     1681 ccgcgttgaa gttggcagag ctggcgaaag atatcttccc ggcaggcgtg attaacatac
     1741 tgtttggcag aggcaaaacg gtgggtgatc cgctgaccgg tcatcccaaa gtgcggatgg
     1801 tgtcgctgac gggctctatc gccaccggcg agcacatcat cagccatacc gcgtcgtcca
     1861 ttaagcgtac tcatatggaa cttggtggca aagcgccagt gattgttttt gatgatgcgg
     1921 atattgaagc agtggtcgaa ggtgtacgta catttggcta ttacaatgct ggacaggatt
     1981 gtactgcggc ttgtcggatc tacgcgcaaa aaggcattta cgatacgctg gtggaaaaac
     2041 tgggtgctgc ggtggcaacg ttaaaatctg gtgcgccgga tgacgagtct acggagcttg
     2101 gacctttaag ctcgctggcg catctcgaac gcgtcagcaa ggcagtagaa gaggcgaaag
     2161 cgacagggca catcaaagtg atcactggcg gtgaaaagcg caagggtaat ggctattact
     2221 atgcgccgac gctgctggct ggcgcattac aggacgatgc catcgtgcaa aaagaggtat
     2281 ttggtccagt agtgagtgtt acgcccttcg acaacgaaga acaggtggtg aactgggcga
     2341 atgacagcca gtacggactt gcatcttcgg tatggacgaa agatgtgggc agggcgcatc
     2401 gcgtcagcgc acggctgcaa tatggttgta cctgggtcaa tacccatttc atgctggtaa
     2461 gtgaaatgcc gcacggtggg cagaaacttt ctggttacgg caaggatatg tcactttatg
     2521 ggctggagga ttacaccgtc gtccgccacg tcatggttaa acattaagtt agatgttagt
     2581 ggctgttata ggacggccac tatcgtttta ggttctgagt ttaatggcgg ttaagtatta
     2641 gaatgattac attaataact agcgtaaaaa ttaggctaaa tgtttgatgg cgatttttca
     2701 tcctgtcaca gttgattagc ataaggcttt agagtatcgt tattaaacta ataatttata
     2761 agtagaagta ttaacaatga aagttcaatt gtacaaattc acggaagata aaaataaaac
     2821 ccttaccttc agatggacga aaaaacactt tgagttttgt atggataata aaatattttt
     2881 aaatcataag gggaagaagt cgtataaaga aaggaattta tttcttttct caaaaggtga
     2941 caagataaca attgaagata atgttattgc tgaagaatat tcaacaatgc ctgtgaaaaa
     3001 tttctccagt gtcggcgctt tttcatttcc gacctgccat ttttcaggga atatacgtat
     3061 aggtcgattt tgtagtattg aataatgggt ggaaatcatc ctttgaatcg ttt
//
LOCUS       DADLSB010000285         3095 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.286, whole genome shotgun sequence.
ACCESSION   DADLSB010000285 DADLSB010000000
VERSION     DADLSB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.286"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            24..800
                     /locus_tag="JN331_004186"
     CDS             24..800
                     /locus_tag="JN331_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HAY7262126.1"
                     /translation="MSAQSVNEIISKIGADAVALPENFRIHDLEKFNLNRFRFRGVLS
                     TASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEPGHADNTATLKLKKTAP
                     FSALLSVNGERHSQKSLAEWIEDWADYLVGFDANGDAIQATKAAAAVRKITIEANQTA
                     DFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRPVL
                     VLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            928..1452
                     /gene="yfdR"
                     /locus_tag="JN331_004187"
     CDS             928..1452
                     /gene="yfdR"
                     /locus_tag="JN331_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7262127.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPTPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPTVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFKELTES"
     gene            1561..2427
                     /locus_tag="JN331_004188"
     CDS             1561..2427
                     /locus_tag="JN331_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262128.1"
                     /translation="MNQNPFSFYDFLGYLIPGGFFILLMYFCGLTFDLDIVIDLTELL
                     RGQSQIFGILNYGSIVIISYIAGHFISITSAFFIEKYMNEELQYPSEYLFNKLSDDKK
                     NVLTPSSDKQNEDTKRKIKYCIIKVMLFPIIPWDYATQKLCYSQSLPFQLANTTWLMI
                     KEGYEKKFRIDDKLLNIRTGLHDDLFRLAYHYVYEFSNQHQTKIQNYVALYGFCRNIC
                     LVFVITFWISFLTLIYRAIEGGAVSYISISTLLSFFFVYVFYVGFVKFYRRYTLEVLM
                     AFAVLQSKDAIN"
     gene            2468..2767
                     /locus_tag="JN331_004189"
     CDS             2468..2767
                     /locus_tag="JN331_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator protein PrtN"
                     /protein_id="HAY7262129.1"
                     /translation="MNTLFLLMAEFNTSNIELSAVSQKYFGMSPATAEAKAKAKAKAN
                     ACKLPVPTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTGKQKGKEHH"
BASE COUNT          955 a          619 c          617 g          904 t
ORIGIN      
        1 caattaatca aatccatgct cttatttctg ctcagagtgt taatgaaatt atcagtaaga
       61 ttggtgccga tgctgtggca ttgcctgaga atttccgcat tcatgatctg gaaaaattta
      121 atttaaatcg cttccgtttc cgtggtgtgc tttccactgc cagcatcgat gactttaccc
      181 gttattctaa agatcttgca gatgaaggca cccgctgctt tatcgatgcc gataatatgc
      241 gagccgtcag tgtgcttaac ctgggtacta ttgatgaacc aggtcacgca gataacaccg
      301 ccactctcaa actgaaaaag acagcaccgt tctctgctct gttgtctgtt aatggcgagc
      361 gtcattccca gaagtcactg gcagaatgga ttgaagactg ggccgactac cttgtgggct
      421 ttgatgctaa tggtgacgct attcaggcaa caaaagcggc tgcggctgtc cgtaaaatca
      481 cgattgaagc aaaccagacc gctgattttg aagataatga cttcagcggc aaacgctccc
      541 tgatggagtc tgtcgaagcg aaaaccaaag atattatgcc agtggcattt gaatttaaat
      601 gcgttccgtt tgaaggtctg aaagaacgtc catttaaatt acgcctcagc attatcactg
      661 gcgatcgtcc tgtactggtt ctgcgcatta ttcagctgga ggcggtgcag gaagaaatgg
      721 ctaacgaatt tcgtgatctg cttgttgaga aattcaaaga cagcaaagta gaaaccttta
      781 ttggtacttt caccgcctga tttcattact gcaaatgccc ctgcgggggc atttatggaa
      841 acgtaattga ctcaataatc gccggatggt gagggcttcc ttttaccaga attcagcgtg
      901 gtgcagcaca tatacgcgga gaacaaaatg tcatttatta aaactttttc cgggaagcat
      961 ttttattatg acaggataaa taaagacgac atcgtgatta acgatatcgc ggtttccctt
     1021 tcaaatatct gtcgctttgc cggtcatctt tcacacttct acagtgtcgc ccaacatgcg
     1081 gtgctttgca gccagctggt gccgcaggaa tttgcttttg aagcgttaat gcatgatgca
     1141 acagaagcgt attgccagga catccccacg ccgctgaaac gccttcttcc tgactataaa
     1201 cggatggaag aaaaaataga cgccgtaatc cgtgagaaat acgggttacc cacggttatg
     1261 agcacgcctg tgaaatatgc cgatctcatc atgctggcaa ccgaacgccg cgatctcggg
     1321 cttgatgatg gctctttctg gcctgtactg gaaggtatcc cggcaacaga gatgttcaaa
     1381 gttattccac tgtcgccagg ccatgcctat gggatgttta tggaacgctt taaagagtta
     1441 actgagtcgt aaaaatcagc acgtacgaat tcaaactctg ccataaaaga acatataagt
     1501 aatttattaa catatagata taggttatat tacaaattga aaaattattg gagagcaaca
     1561 atgaatcaaa atccattctc attctatgac tttcttggat atttaatacc aggtggtttt
     1621 tttatcctat taatgtattt ctgtggtttg acattcgatc tagatattgt tattgactta
     1681 actgaattgc tcagaggtca aagtcaaatc tttggcattt tgaactatgg ttcaatagta
     1741 atcatatctt acatagctgg acactttata tctatcacat ccgcattttt tattgaaaaa
     1801 tatatgaacg aagaattgca atatccgtca gaatatctat ttaataagtt atcagatgac
     1861 aaaaaaaacg tcttaacacc atcaagtgac aaacagaacg aagacacaaa gagaaaaata
     1921 aaatactgca taatcaaagt aatgttattc ccgataatac catgggatta cgccactcaa
     1981 aaactatgtt attcacaatc attgccattt caattagcta ataccacatg gctaatgatc
     2041 aaagaaggtt atgaaaaaaa attcagaata gacgataaat tattaaacat caggacaggt
     2101 cttcatgatg atcttttcag attggcttat cattacgtct atgaattttc taatcaacat
     2161 caaacaaaaa ttcaaaacta tgttgcatta tatggttttt gcagaaatat ttgtttagta
     2221 tttgtaatta cattttggat atcatttcta acattaattt atcgtgctat agaaggtggg
     2281 gcggtgtctt atatttctat ttcgacgcta cttagctttt tcttcgtcta tgttttttat
     2341 gttggattcg ttaagtttta tagacgatat actttagagg tattaatggc atttgctgta
     2401 cttcagagca aagatgctat taattaatat attaccctcg ggtgcagccg ggataatgga
     2461 gaaatttatg aacaccttgt ttttactgat ggctgaattc aatacctcaa acattgaact
     2521 ctcagcagtt agccaaaagt actttggtat gagtccagcc acagcagaag caaaagcaaa
     2581 agcaaaagca aaagcaaacg cttgtaagtt gcccgttcca acatatcgca tcggcacatc
     2641 acaaaaagca aaacgttgca tcaatattca ggatcttgcg gaatacatag acaaaagacg
     2701 agaagaagga cgtatcgagt gggaacaggt cagaacaggc aaacagaagg gcaaagaaca
     2761 tcactaaaga aaaaacccgc ctaaaggcgg gttttcaaaa agcaccagct atgatcatgc
     2821 tgctttgcga cgacgaagct taccctgctg ctctttacca gagacagtag cgtgagtgaa
     2881 cgcattagga gcagccttca tcagaacttc aaagcagccc ccatacctgc gaatgctttc
     2941 attgtgtcga acttaagctg tggcttggtt gctttttgat cttccataga aaactccaga
     3001 agttataccg aaacaattcc tgttgtttac tcatcatcaa tagatgatac gcaatattta
     3061 tttttaaatt taaggttctt tggcgtaact tcatc
//
LOCUS       DADLSB010000286         3055 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.287, whole genome shotgun sequence.
ACCESSION   DADLSB010000286 DADLSB010000000
VERSION     DADLSB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.287"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            368..2020
                     /locus_tag="JN331_004190"
     CDS             368..2020
                     /locus_tag="JN331_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7262130.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPKITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="JN331_004191"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="JN331_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2280..2393)
                     /locus_tag="JN331_004192"
                     /pseudo
     CDS             complement(2280..2393)
                     /locus_tag="JN331_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2512..3035)
                     /locus_tag="JN331_004193"
                     /pseudo
     CDS             complement(2512..3035)
                     /locus_tag="JN331_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
BASE COUNT          871 a          662 c          717 g          805 t
ORIGIN      
        1 atcggaaacg atgatctggc agtctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc aaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg gaaaacggaa ttttcttttt acacccccaa
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg gaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccgtat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctattgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accggacgtg aacctctcag ctcatccggc
     2521 tcttctcatt gaggcctgtc cccatagtaa cggccagtgt gcaaacaacc tcgtttcact
     2581 ccgcgcaaca cgggccagcc agtggctggc ccgtcgtcgg tggcccctaa gtcgcttgtg
     2641 tttacggggc agccataaca cgagtttgcg atcgatttgc ctcagcgtca gatacagtgc
     2701 tgatttataa aaagcaccgt agtactttat ccagcctctg attttcgcat tgaacatatg
     2761 cgccagctcg tacaaggact tgtcactgcg attctcctca cctcctgacg gattgctttt
     2821 cctgctctgg cactcatcgc cggactgaag ttaacgaagg tcttacccca gcgattcatc
     2881 gacagacgcg gccggaatgt atagcccaga aagtcgaagc tcgtttccgg gtagtccccc
     2941 cgtcggtcag catccttaca gtaaactatt cgtgtttttt gcggatgcag ctgcagcccg
     3001 cattgcgcaa gccgctgcct cagtccactt atcagagcgt cagcctgcca ctggc
//
LOCUS       DADLSB010000287         2966 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.288, whole genome shotgun sequence.
ACCESSION   DADLSB010000287 DADLSB010000000
VERSION     DADLSB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.288"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..298)
                     /locus_tag="JN331_004194"
     CDS             complement(<1..298)
                     /locus_tag="JN331_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262131.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEEL"
     gene            complement(298..1008)
                     /locus_tag="JN331_004195"
     CDS             complement(298..1008)
                     /locus_tag="JN331_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262132.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FTVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1005..1993)
                     /locus_tag="JN331_004196"
                     /pseudo
     CDS             complement(1005..1993)
                     /locus_tag="JN331_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
BASE COUNT          679 a          877 c          715 g          695 t
ORIGIN      
        1 tcagttcctc aggtaacagc cagtagagcc ggtaccggga tccgagcccc cgccagtcag
       61 ggtcactatt cccctccgtg tcggtaaaaa acaggtcccc ctgaaacggc aggtatccgt
      121 accggacaat ccggttatta ttcagacaca gcacgccata caggcacggt tcaccattaa
      181 caatgatatc gatatacatc cccgtagtac gctgattcag gcgaatggtg cagacctgag
      241 cgcccagtgt caccgtaaac tgctgggctt taacgggaga taaaacaatt tccagcatca
      301 ggtgatcccc ctgttcgtgg cgcttcgtct gtcagcgtct gacggttgtg tcaccgacgc
      361 cgtaactggc tgcgttttaa ccgatgcggc cccttttgct ttatcgttgt ccgtgggaga
      421 cgacttatcc gtacttccca ctgacacctc tcctgtattc attacggtct gaaataccgc
      481 agtaaccgtc agtaaggtcg gaccattatt acttcgcatt cggtagtcat atttcacaag
      541 gtcgcaggat gtccatgtct tgtccggcgt ctcaatatcg tagagtcctg ccgtggtacg
      601 catcctttca agcgtttcca gcacattcgt ccgcgaggtg gtggaaaaat ttgtcaggtt
      661 cgggacggat ccggaaaagg ccgtccaccc ctctacagtg aaagtcacat gcagttccgg
      721 cgatctctgg attttattaa aggtggtata agccccctgc tccacgggga cggtggaaac
      781 agaagcctcc gcgcccacct caacgacaac aaaagaatcc ggggagaaag gtttgccacc
      841 cttctgatgg gcacctgccg gatcattccg tgcgtaataa ataccgaatg acggtgccag
      901 tacactgtta atgagtccca ggacaccgcc gccacgaact gcactcagta cgttactttc
      961 actgagcgaa aagttattca gggaaatatt atcgaaagaa aaactcatcc tgttaccccg
     1021 ctggaataaa ccgacacaag cgccgaattc gtgatacgcc gacgtgcgtc atcggtaata
     1081 cccttcacat tgtccgacgt tgtggtgaca ttcagtgtcc cgatatgcgt ggtttccgtt
     1141 acggtggact gcgatacagg cgcaggatgg cgcgaccgta tggccattgc cgcacccgga
     1201 taaggcaggt tcgccagcac ccgcggaata tagttgcggg tctcctccgg agcagcagcc
     1261 agtcccttac gctgaacatt tccctctccc cagttatatg ccgccagagc cttagccaga
     1321 tcgccatgaa aaaaacgtat caggccacca agttttctcg cggcggcatc agcggctttt
     1381 tcgggatcaa aggcatcgtt ccccctcaga ccaaattcac tggccgtctt cggcatgaac
     1441 tgaaacagtc ccatcgcacc agcaggtgag acggcaaact gattaccacg ggattcggtg
     1501 atcgcaacgc tgcgcagcag tccgggcggc aggttatatt tttcctccag ttgagacagt
     1561 ttcggttgca gccagcctaa cagggcttcg tcggtcgctg gcggttttgc atggcattac
     1621 cgagtttttc ctgcgtcgca cgcataccct gtagccagga ttcaccgaag gctgcttccc
     1681 tcccggttgc ctgagaagcc tgagtatcca gcatgccctg ctgccatact gtgggtgatt
     1741 gtgcccgggt gatgttgcca gacttttctc cggcatccag ttttgtctga tacgtctcca
     1801 tctctttctt attgaaaaag aaagtcccgt ctgaagccca gaaaaaacca tgtgaatcca
     1861 gccagtcctt attttccctg ccaacaatgg atgtcagtaa tccgtcaaca accgggtaaa
     1921 gcgccgttat cgcaaaaaga aggcctccgg ggccactgag ggcttttgtc agcccccgca
     1981 cccatgacgc cactttcagc ccgatcagcg taataatgac attctgccag ccccccagtt
     2041 ctccggcagc cttattcgcc agggaggcca ctgactcaac gtcattcaga aatgtggtga
     2101 taaacctgtt cacttcctcc ggatgttgct gcatccagtc accgagtgtc tccagccagt
     2161 ggctgagctt cagcgcgtac ggcatcaacg cggtgctaat cgtcatcccg atgccggacc
     2221 aggtctgatc gagctcaaca agctcttttc gtagcttacg ggccagcgcc atacgccccg
     2281 gcgtaacctg cgaacgggaa gtaaattcgt ccacatcttt cagcgcatga ccggagccaa
     2341 gaaactgctg tccggcataa ctgaagccca gcgcgttgcc gtaggcagtt tgttcggact
     2401 ttgtcagtcg cggaaaaata accgccagct tgcgcatgat ggcttcagtg ctgtcagtat
     2461 ttaaatcaac actaatgccc gccctggctg caacctgaaa caaatcctgt aacacaggat
     2521 caaaggacct tccggctctg aaggccgctt ttgcatttgc aagccgggaa aatgccccag
     2581 acatttcccc ttcatcaaca ttatttgccc gcgcagcccg taaccatccg tccagatttc
     2641 tggccttcat gccaaaagca tcagattcca cggacagacg gttcagatca cgggcaaaac
     2701 ctgttaccag gttcttcagc ccccccagcg tcagactgac acccgcaaga gccagaatct
     2761 cgctacggat ccctgaaaaa aaacgggacg cccgtttccc ggcatcttcc attcgacggg
     2821 ccgttttatc agcatctttt ccggtttttt ccagtgattc actggttttc ttctgtccct
     2881 gactgaatgt gtcagcgaca ccttccatca ccgtcgtgag ccggttaagg gcagcatgta
     2941 ctgcctgttc cccggcagta aagtcc
//
LOCUS       DADLSB010000288         2964 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.289, whole genome shotgun sequence.
ACCESSION   DADLSB010000288 DADLSB010000000
VERSION     DADLSB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.289"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(55..507)
                     /locus_tag="JN331_004197"
                     /pseudo
     CDS             complement(55..507)
                     /locus_tag="JN331_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(523..1419)
                     /locus_tag="JN331_004198"
                     /pseudo
     CDS             complement(523..1419)
                     /locus_tag="JN331_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(1419..2024)
                     /locus_tag="JN331_004199"
     CDS             complement(1419..2024)
                     /locus_tag="JN331_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY7262133.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(2195..>2964)
                     /locus_tag="JN331_004200"
     CDS             complement(2195..>2964)
                     /locus_tag="JN331_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707671.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY7262134.1"
                     /translation="LQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPK
                     ADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVVL
                     KELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWW
                     STLAKSNLTFFKFGALSNRHTPPAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPL
                     LALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT          894 a          738 c          617 g          715 t
ORIGIN      
        1 ctaccgaaat atggtgctgg tttattagca gtaattttgt ggggatccct caggtgccag
       61 cggcacatac aatttaacct taaaggccgg agaagccagc accagcccac gaattgcagg
      121 tgcataatca tgaacccatg tggcaacaag cactgcgccg acgctttgcg cgatcactac
      181 cacctcttcc agtccgacct ggctgtcgct ggcagcaaaa cggacaaatt catcgacatc
      241 ctgcactgag cgcataagag acgggctata accacgcggc cccgaatttt ttccatgccc
      301 ccgggcatcc caggcataaa aagcagtatc aggcatcgcc agttcatcaa cgagatgttg
      361 tagacgacca gaatgttcat gcccgcgatg aaataagacg atgacctttt tcgcccctgg
      421 ctgtaaagtg ggccagtgcc gataatacaa cgctgtatta tcactggtgg taaaaaaatg
      481 ttccccaggg atgcgtgaat tttccatttt ctctattttc cttcagtaac agcagtagcg
      541 aataaaataa aaaaataccg gagcggtgaa aattaatgag tcaatcctgt caaggagtcc
      601 gccatgtcct agtaatagtt ttccactatc tttaacacca atatctcgtt tgatggctga
      661 catcactaca tcgccgcaaa aaccactaat accaattaac aaacccgcta ataatgcctg
      721 taatgtattt agcggtgtca gcaacggtcc aataatcagt gacgcaatca tggtggtgat
      781 tacgcccccc agcagacctt ccagcgtttt tcccgggctg actttgggga ccacttttct
      841 tctgccgcag gatttccccc ataaatactg tgcaatatcg tttgactcgg ttaacgccaa
      901 aagaaataac accagtaacg ccccgccctg gatatttatg gttggcaaca ttaatagcca
      961 ggcggcatga ctcaaggcaa aaacggtcat tatccatccc caaaaaatgg cgctcacggt
     1021 atgctgaaaa ccggaggggt cacccacaaa aactcgccag gtggctaata tcagaaaccc
     1081 agtcaaaggt ataaataaca gaaacagctc aaagcaatta aaaccgataa gcaaatagtt
     1141 aagaggaata acccaatata accaacgcga aaaatgtacg gatataagcg tacaatattc
     1201 tttcaatgcc agaaaactta tcagcgtaaa aaacgtcaac gtcatccagc gtgggcctga
     1261 aatcaccagt gaaaaacaaa tgacgatgcc ccactaggta cgaatacgta atgtaacttc
     1321 atcccctttt ctctcaggta accgccatag cagaaaacgg tttatcagcg ttgctaaaat
     1381 taacaacgcg aaaagtgttg ccagagattt ttccagcatt atctttcagc gctcataaga
     1441 agcacactcc gacaacgatt aatcgcagta cagataagca gaattgaagc aatgctccat
     1501 aaaagattat tccattgcat ccactgtgga taaatggcaa cggccagacc ccacagacca
     1561 aatattaacg cgcgatcact tttgccaaat ggaccggcat aactgcgaac accgttaatc
     1621 gtctgcgcga gtaaaccgca aaactcggtc aatatggtgc aaaataacat gagtatcacg
     1681 agtgatgcgt tactttccgg taaaaataaa aacggtaaat agagcgcaat atcggaaata
     1741 acatcgccag tctcattcaa aatcgccccc agacgtgttt gctgattgca ctcacgcgcc
     1801 aacatgccat ccagcgcatt gagcgccata cggataaaaa gcacgatggg caatagcaaa
     1861 aagaggatgg gttgtgccac caacaccaac agcaatccgg taaaaagaga aagcgccagt
     1921 gccgtaagag tgatgtgatt cgctgtaacg tggtgcttat aaagccaaaa catcgtcggc
     1981 cttaacagcg actgaaagag cggttttatc tggtagagcg tcattatcaa tccttgataa
     2041 ttcggtttgc gctatggtgc atcaattccc ctgacggaga tatcgcaaca cgccgcttca
     2101 ttaaaaattc agaatatatc aaggtattgg gggctcactg tcaggaatga ttatccccaa
     2161 taaatcaagt agttaaaaat tattacgctg ttattcaata caactcatca agtttcctca
     2221 tcgcttgttc ccgtattgcg cgcgtcttac cgttaagcgc cagctggcgg actaaatcca
     2281 gttcagggtt aacaagctct aacgccagca aaggatcacg tttcagcctc gccttcaata
     2341 catcaactgg aaagcgggga ttattcatcg ccacaatcca ccactcggga tcaatttctg
     2401 ccgccagtgc cgcagggggg gtgtggcggt tgctaagtgc gccgaacttg aaaaatgtga
     2461 gattgctttt cgccagtgta ctccaccata tacgatccgg ttgttttgcc agtacatcca
     2521 gttgtgctaa cgcatcgggg tcgagtgcat aataaacacg taatgcttca tcaacctgcg
     2581 cacgggagct attttcctgc ctcaggaaaa acttcgccca aatatcattg cgtggcgtgt
     2641 tttgctgtaa ttccttgaga acaacagcgg gtaaattatc ccaactaact aatgcactct
     2701 ctaaaaagtc ccagtcttcc aaacgtatca gggcggcggc atttaatagc atctggtcat
     2761 caacttcgcc gtggctaatc agctcagaca aaatcatatc agcgctggtt tcatatgcat
     2821 ctgaatctgc tttcggatta tttttccgat gcgataaagc acgttgccaa attttttgtt
     2881 tcatcgacgt gctcagatac tgatttttcg ccagattact ccacccctca ggatcgttga
     2941 aacgtgtcaa taaccgttgt aaag
//
LOCUS       DADLSB010000289         2935 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.290, whole genome shotgun sequence.
ACCESSION   DADLSB010000289 DADLSB010000000
VERSION     DADLSB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.290"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            246..1859
                     /locus_tag="JN331_004201"
     CDS             246..1859
                     /locus_tag="JN331_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7262135.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYFAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1910..2935)
                     /gene="ynaI"
                     /locus_tag="JN331_004202"
                     /pseudo
     CDS             complement(1910..2935)
                     /gene="ynaI"
                     /locus_tag="JN331_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
BASE COUNT          850 a          645 c          684 g          756 t
ORIGIN      
        1 gcgacagatg ctgattatga taccgaaaac tggtttgaac gtgcgaagcc cgaacgtagt
       61 gttcggtgtt ctatgtgctt tgccgcattt tggcgtctat tatttacact aaatctgatt
      121 tgatatattg atacttaaaa catttgatgc ttcctttgtc acttttttga tggaagttgt
      181 ttgcatttct ttaaggcgaa acaaataatt acgcatcaat tttaatgtcg gttagaggga
      241 aacttatgaa gcactctgtt tcagttacgt gttgtgcgct gttggtcagc agcatttctc
      301 tttcgtatgc tgcagaagtt ccgagcggca cagtactggc agagaagcag gagctggtgc
      361 gccacattaa agatgagcct gcgtcgctgg atcccgctaa agccgtgggc ctgccagaga
      421 ttcaggtcat tcgcgatctg tttgaaggtc tggtgaatca gaacgaaaaa ggggagattg
      481 tccccggcgt cgcgactcaa tggaaaagta atgacaaccg tatctggact tttaccctgc
      541 gcgataacgc aaaatgggcg gatggcacac cggtaacggc gcaagatttt gtctacagct
      601 ggcaacgtct ggtggaccca aaaacattgt cgccatttgc atggtttgcc gcgctggcgg
      661 gaatcaacaa cgcacaggcg attattgatg gtaaagctac gcctgatcag cttggcgtca
      721 ccgcagttga tgcccatact ttgaaaattc agcttgataa accgttgccg tggtttgtga
      781 atttaaccgc taactttgcc ttcttcccgg tgcaaaaagc caacgtagaa agcggtaaag
      841 agtggacgaa acccggaaat ctgatcggca atggcgctta tgttcttaaa gagcgcgtag
      901 tcaatgaaaa actggtcgtg gtaccgaata cccattattg ggataacgcc aaaacggtgc
      961 tgcaaaaagt gaccttcctg ccaattaatc aggaatccgc agccactaag cgttacttcg
     1021 cgggggatat tgatatcacc gaatccttcc cgaaaaatat gtatcagaag ctgttgaagg
     1081 atattccggg gcaggtttat acgccaccgc agctcgggac ctattattat gcctttaaca
     1141 cgcaaaaagg gccgacggca gatcagcgcg ttcgcctggc attaagtatg acgatagatc
     1201 gccgcctgat gaccgaaaaa gtgttgggga cgggcgaaaa gccagcgtgg cattttatac
     1261 cagatgttac cgccggattt acgccggaac cttcgccgtt tgaacaaatg agtcaggaag
     1321 aactgaatgc gcaggcaaaa actttgttga gcgcagctgg ttatggtccg caaaaaccgc
     1381 tgaagctgac gcttttgtat aacacttcag aaaaccatca aaaaattgcg attgctgtag
     1441 catcgatgtg gaaaaagaac cttggcgtag atgttaaatt gcaaaatcag gaatggaaaa
     1501 cctatatcga tagccgtaac accggcaatt ttgatgttat ccgcgcctca tgggtgggtg
     1561 attataatga accctccact ttcctgacat tattaacgtc aatgcattca ggaaatattt
     1621 cacgctttaa caatccggca tacgacaaag ttctggccca ggcatcgacg gaaaataccg
     1681 ttaaagctcg taatgccgat tacaacgcgg cagaaaaaat cctcatggag caagcaccga
     1741 ttgcaccaat ttatcaatat accaatggac gattaatcaa accgtggctg aaaggttatc
     1801 ccattaataa ccctgaagat gtagcgtata gtcggactat gtatattgtg aagcattgat
     1861 gtgatgggaa ctggcgttac ccttgtggat aacgccagtg atgtctgatt tagcgaccct
     1921 gttccggcgg tgtaatgtta tccatataca gcgtctggct ggggaaggca aagtccgcgc
     1981 cgtgtgactg tacaatatcg ataatcttca aataaacgtc ttgctgtgca gcaagccact
     2041 cagcccatac cgtggttttg gtaaagcaat aaaccataat attcaatgac gagtcagcaa
     2101 actggttgaa ataaaccagt aaggtttgtc gctggtcgat ggccgggtga tttttcagca
     2161 tctcacgtac ggcttcgaca ataacgccca cttttgccgc atcctcataa cgtaaaccaa
     2221 tggtcgtggt aatgcggcgg ttggtcattc gtcctgggtt ttctacgctg atcgacgaaa
     2281 acagcgagtt cggtacgtac aatggacgat tatcaaaggt cgtaattttg gtaattcgcc
     2341 agccaatttc cgctactgta ccttcgatat ttctgtccgg tgaacggatc cagtcgccaa
     2401 tactgaaagg acggtcgaaa tagagcatga tcccggaaaa gaaattactc agaatatctt
     2461 taccggccat accgacagcc agaccaccaa taccaccaaa ggtcagcaag ccagaaaggc
     2521 tcatgccgaa atgttcgcca taaagcagaa caagcactac aataatggtg attttgataa
     2581 tgcgcgacat aatccgcgca ctggtgatat cgcgaccttt tttaatctgc tgtttttcaa
     2641 actgattaat cagcagaaat agcttaatcg tcagaataac cgcaatcagg gacgtacaga
     2701 taaaatcgat aacgcctggg gtgataaatt tgagtttata gttttctata acataattaa
     2761 tgatgctacc aacagcactg ataattatgg tttagattaa aaattgcacc gcatggaata
     2821 aaaatccttt tcttttacga tttccacggc gaaaccaaaa gctcatcaga atcaatgctg
     2881 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagc
//
LOCUS       DADLSB010000290         2918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.291, whole genome shotgun sequence.
ACCESSION   DADLSB010000290 DADLSB010000000
VERSION     DADLSB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.291"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..178
                     /locus_tag="JN331_004203"
     CDS             <1..178
                     /locus_tag="JN331_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262136.1"
                     /translation="GASLSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGK
                     INQTTTKMLFMCRE"
     gene            complement(286..687)
                     /locus_tag="JN331_004204"
     CDS             complement(286..687)
                     /locus_tag="JN331_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY7262137.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            929..1222
                     /locus_tag="JN331_004205"
     CDS             929..1222
                     /locus_tag="JN331_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262138.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1294..1953
                     /locus_tag="JN331_004206"
     CDS             1294..1953
                     /locus_tag="JN331_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY7262139.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            2030..2491
                     /locus_tag="JN331_004207"
     CDS             2030..2491
                     /locus_tag="JN331_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY7262140.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2698..>2918)
                     /locus_tag="JN331_004208"
     CDS             complement(2698..>2918)
                     /locus_tag="JN331_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HAY7262141.1"
                     /translation="ANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLK
                     EAAKKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT          758 a          607 c          721 g          832 t
ORIGIN      
        1 aggggccagt ctatcattac aaggctacga tcccagcgaa tttacattcg ctaacggtgt
       61 attttgcgat gttaaagaaa aattatgtcg tgatgatcgt tattttggtg tagatggtaa
      121 gcggagtgga aaaatcaatc aaaccaccac aaagatgtta tttatgtgtc gtgagtgatg
      181 ccttgtttac attatgttct ctgctattcg tcattaacct cttgagcgac agagaggagt
      241 ggggcgttaa cgtctatgac ggggatagtc cccgtcggtt gacatttact taatttgaat
      301 atcgacgtta tattttttag ttttgttttt acgggcgtcg tttcgagttt gcagcactcg
      361 cagctcatat ttgccagaag cagggagtga gtattgtccg tgactatcga gttctgggga
      421 atatctggat aaatcaacag aatcatcgat tcctggcccg aagagataag tatcagcgcc
      481 ttcattagag atactcacat gcactttctg accttttttg gcatagaagg tatatgtatc
      541 gtaatcgtat ccctttattt cgccggagta ctgagcgctg ctatggcctt tgctgaactc
      601 aacattgaca ttcttacccg cagcaaagct cgtggaggtt agcagtgcaa gcagcaatac
      661 agccttactg atgctcttga ttttcattgg tagtctcctg ttcagaatga gtacgatgaa
      721 ctgttataat ataacaatcc cttacgggag aattccagtg gcttttctgc gttcggtaaa
      781 atcccggcgc ggcatttttt gccgggggga ttacttataa aggggcatgg actcaacaaa
      841 ccgcagaaat ctacagctgg ctggcttgtg gcgcgggttt cattgatgtt agcttatgcc
      901 aaaacctggc atcttaaaga gtaaaagtat gttttgtgtg atttatcgaa gcagcaagcg
      961 tgaccagacc tatttatatg tcgaaaaaaa agacgatttt tcgcgtgttc ctgaggaact
     1021 gatgaaaggt tttggtcagc ctcagttagc gatgattctg ccgttggatg ggcggaagaa
     1081 actggtcaat gctgatattg aaaaggttaa actggcatta accgagcaag gttactattt
     1141 gcagttaccg ccaccacccg aagatttgct gaagcaacat ctttccgtca tggggcagaa
     1201 aacagacgac actaacaaat aaccgatatc cggcggtggc attatctttg tcggcgcggg
     1261 ttttcatatc cacgataagg tgaggggaac gttatgtatc aacatcacaa ctggcaaggt
     1321 gcgctgctgg attatccggt gagtaaagta gtctgtgttg gcagtaacta tgccaaacat
     1381 attaaagaga tgggcagcgc agtgcccgaa gagccagtgc tgtttattaa accagaaacg
     1441 gcactgtgcg atctgcggca gccattggcg atcccatccg atttcggttc agttcatcat
     1501 gaagtcgaac tggcggtgtt gattggcgcg acgctgcgtc aggcaacgga agagcatgtc
     1561 cgcaaagcca ttgccggtta cggcgtggcg cttgatctga cattgcgtga tgttcaggga
     1621 aaaatgaaga aagccgggca gccgtgggaa aaggctaaag cgtttgataa ctcttgtccg
     1681 ctttccgggt ttatccccgc ggcggaattt accggcgatc cacaaaatac aacgctgggc
     1741 ctgagcgtaa acggcgaaca acgccagcaa ggtacgactg cggacatgat ccataaaatc
     1801 gttccgttga tcgcttatat gagcaagttt tttaccctca aggccggtga cgttgtgttg
     1861 acaggcacgc ctgatggcgt cggcccgttg caaagcggtg atgagctgac agtcactttc
     1921 gatgggcatt ctttgacaac tcgcgttttg taatactttt tgccgccaga aagcggcggc
     1981 aaaacttgca tcgctgtgcc agactggtta taaggtgcgt tttaacgtaa tggcggaaca
     2041 cctgatgagc gatgtacctt tctggcaaag taaaaccctg gacgaaatga gcgatgcgga
     2101 atgggagtcg ttgtgtgatg gttgcggtca gtgttgcctg cataaactga tggatgaaga
     2161 caccgacgaa atctacttca ctaacgtcgc ctgtcgccag ctcaatatta aaacctgtca
     2221 atgtcggaac tacgaacgtc gtttcgagtt tgaacccgac tgcattaaat taacccgtga
     2281 aaatctgcca acattcgaat ggctgccaat gacctgcgct tatcggttgc tggcggaagg
     2341 taaagattta cctgcgtggc atccgctact tactggttcg aaagcggcaa tgcatggtga
     2401 acgtatctct gtgcgtcata tcgcagtgaa agaatcagaa gtgattgact ggcaggatca
     2461 tatcttaaat aaacctgact gggcgcagtg ataaatttaa tagatgctga tgggggcttt
     2521 cttaatatac gtctggatgc tcgccatttc gtagccctgt ttttgaaagc gctctgtcat
     2581 aatgagagtt acccgatacc ttattatcaa caggtaactc tcaatcttta aaaaacgtct
     2641 gtgtttttaa atgtgataaa ccttatacct tgagtacatg gagagaataa tcgcttatca
     2701 gacttcaggt acctcaaaga gtgtcttttt accgtgtctt ttctgatact cattacaggt
     2761 gttaatcatt tttttggccg cttcttttag caaagaaagc attaaatcat cataatcaac
     2821 atagaatctg gttgtttcag tttccgtttt tatctccccg atggcggcta tttcggtggt
     2881 taatttcaat ttggcggcat cgatatcttt attcgctt
//
LOCUS       DADLSB010000291         2848 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.292, whole genome shotgun sequence.
ACCESSION   DADLSB010000291 DADLSB010000000
VERSION     DADLSB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.292"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(254..1051)
                     /locus_tag="JN331_004209"
     CDS             complement(254..1051)
                     /locus_tag="JN331_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7262142.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1061..1612)
                     /gene="ybcL"
                     /locus_tag="JN331_004210"
     CDS             complement(1061..1612)
                     /gene="ybcL"
                     /locus_tag="JN331_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY7262143.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKISVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1781..2113)
                     /gene="emrE"
                     /locus_tag="JN331_004211"
     CDS             complement(1781..2113)
                     /gene="emrE"
                     /locus_tag="JN331_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY7262144.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2458..2772)
                     /gene="fliE"
                     /locus_tag="JN331_004212"
     CDS             complement(2458..2772)
                     /gene="fliE"
                     /locus_tag="JN331_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY7262145.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
BASE COUNT          840 a          637 c          552 g          819 t
ORIGIN      
        1 cctttaatta tcccctcaac ctcttgtaaa tttcccactc ttgagctcca catagtccaa
       61 acgccggatg caaaaaaaat cacacgtgca tctctgaaat tgtgatggag gaagtgagta
      121 tcaattatat gtgcttataa acgttcccga ttggcacaga agtcaccgga ttgttcaggc
      181 tccaataggt tgagtatgtc gtatttattt tccaaaataa aaatcgatgc tgttgctcct
      241 taataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt atcttaaaac
      301 gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc ctggcaatct
      361 cgctgatggg ttttactgtc gtttgtatag cagacaacgc attatttaac ctcacgtcgt
      421 ccaatatact ttgaaaactt attccctcgc ttgccagacg gcgatgcaat gtggaaacag
      481 aaatgtagag atatctcgct accttgtttg ctgtccattt tgttccaggt tcggatagca
      541 ttaggttata acaacgactt attaatgatt gtttactatc tgataaaagt aaacaattaa
      601 catgatgtgc tcctaatgaa agtagaacgc ccattgctaa gtgttcctga atttgcgttg
      661 agaagcctcg ggaaatagat gtttttagtt gctcccaaca atatattaac tcggggttct
      721 gaggtaagca gaaacctgtc gtgttacgaa tttgatcagt tatcgcataa aatttttgaa
      781 acttttcaat tagatcaatg ggataataaa gcatttctgc aagataaaga cctgcttctg
      841 gataattcac aatataaaat tcatatccac agggaaataa tattatctga ttattatcaa
      901 cagttagact atgcgtctcc caattgataa ctttttttcc ctgacggata cgacataaag
      961 ctggcataag aggcttaacc ctgtgaatct catgatgttt atgcatccgt atttcttcaa
     1021 tctttaggtt agacttatct tttgccagca tactcttacc ctactttatc tcataaactg
     1081 gtgttatctc agcggttgca attttattgg cattaagcat ataaccaact aacgctccgc
     1141 tggaattaga atccacagaa atcttatcag tttttagagc ccatacttta aactggtaat
     1201 gatggggttt atctcctttg ggaggacatg ctccaccaaa tcctgcataa ccaaaatcat
     1261 ttcggccttg aacagcacca gtcggaagtt ttgtaccatc acgtcttcct gcatcagtgg
     1321 gcaaatatgt tacagttgct ggaatattag caacagtcca gtgccaccat ccactgcctg
     1381 taggagcatc tggatcatac acagttacgg caaagctttt ggtgccttca ggagcaccag
     1441 accaggttaa tgagggcgat gtattaccac cttcacaccc aaatccagaa aatacatgag
     1501 acgttgtaag ttgctctcct gtttttattt cattactagt gacctgaaat gctgcggcct
     1561 gcgcagaaaa tgttatgaat gccaatacac ttgaaacgat aagttttttc ataaaaacct
     1621 cattgttgtg acctatcgtt attttatttg atgtgccttg atctcattat gcataaaggc
     1681 gcaatgatcg cgcaaatgca atcataatta taaaggtgcc ccgaacatta gccataatat
     1741 gcacaaatca gattcctgtt atctcaagga taattatttt ttaatgcggt gcacttcgtg
     1801 acaacaagtt aatgactaac acaccggcac aaatcaacat catgcctatg atagctggca
     1861 ggtccagtcg ttgaccaaaa aatccccagg aaagcaaact aatcagaacg ataccgactc
     1921 ctgaccagat agcataagca atccctgtag gtatataagc cagcgtctga gctaataacc
     1981 agaatgatgc acaatagcaa ataattgtgc caacagaggg ccataaccgt gtaaacccct
     2041 ctgaaaactt cattaaggtt gtacctatga cctctgcaag tattgcgcca ccaagataaa
     2101 tataagggtt catagcatat tctttcctgt tcatactgga gagaattgta ctacagtttg
     2161 aacacaactc accgttttca tcctggtgta tgtgtatcca aggggctctg gcgtggcaag
     2221 agataaaggc aggaacaata atgatcagaa ggaaccccag gctaacgaat gagttcactc
     2281 ttcttgggag caaagcgaag acaaaacatg ttatcgcgtt tacggatcgt ggatggcaga
     2341 aaacaaccag gatcaggtat ccattttaaa ccagaaattg agtgagtttg ccccatccat
     2401 gccccacgct gtgggatcgg atataattaa acaagcatag tcaacaggtt acacaaccta
     2461 cacctgcatg gacatcactt cctgatacgc cgccaccagc ttattacgca cctgaatccc
     2521 catttgcata gaaactgagg ctttttgcat atcggtcatc acatcgttta gcgccacgcc
     2581 gggttcaccg agggtgaatt tttctgcctg cgtgcgggcg actgtttgtg tatcgcttat
     2641 gcgatcgagc gcggcgtgca gctgcccggc aaaactaatg gtcggttgcg gcagtgattc
     2701 ctgcgcacgc gcactcatcg ccgtagcctg taactggctg ataacccctt caatcccctg
     2761 tatcgctgac attttcgtct cccggataat ttctggtaac aaagcctacc agtaagtcaa
     2821 taagacaaag gcgctaaata gcaacaaa
//
LOCUS       DADLSB010000292         2783 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.293, whole genome shotgun sequence.
ACCESSION   DADLSB010000292 DADLSB010000000
VERSION     DADLSB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.293"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            347..1690
                     /gene="argG"
                     /locus_tag="JN331_004213"
     CDS             347..1690
                     /gene="argG"
                     /locus_tag="JN331_004213"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY7262146.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(1698..>2783)
                     /locus_tag="JN331_004214"
     CDS             complement(1698..>2783)
                     /locus_tag="JN331_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7262147.1"
                     /translation="FATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT          737 a          651 c          672 g          723 t
ORIGIN      
        1 ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg
       61 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca cattttgtca
      121 acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt gaatactttt
      181 gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg
      241 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
      301 cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
      361 caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc tggacaccag
      421 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
      481 gggccagcca gacgaagagg attatgatgc gatccctcgt cgagccatgg aatacggcgc
      541 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
      601 tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca cgacgccgct
      661 gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat
      721 ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc gttatggtct
      781 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
      841 actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
      901 gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc atgaagcgaa
      961 ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg gcgtgaaatt
     1021 ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg agcaaggtca
     1081 tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa
     1141 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc gtatcatcga
     1201 agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca ttgcgtatga
     1261 acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg cgcatggtcg
     1321 tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc tgatgctgcg
     1381 tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc tggagctgcg
     1441 ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct acaagccaga
     1501 gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta ttggtcaatt
     1561 gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt atgccaaaac
     1621 tggcctgctt tcctcctctg ccacttcagg cgtgccgcag gtggagaata tggaaaacaa
     1681 aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg cttatcctct
     1741 gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc cacaacccgt
     1801 gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc cggctttgtt
     1861 acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat attgttttcc
     1921 gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat cgggacatga
     1981 tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt cgtagggtca
     2041 cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt taataattca
     2101 aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca ggcatcctga
     2161 tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc cggttcatgg
     2221 cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg taatgcttgc
     2281 gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc catggcgcgc
     2341 atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga gcttagccag
     2401 aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta attatgaatg
     2461 tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt
     2521 gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc aacttgcggt
     2581 gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga ctcccccaca
     2641 atcaacacgt aggtatcaat acctgtatcc ctgactgata attgaaaata cggcaccgtg
     2701 tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata gttgagattg
     2761 aaaaacggcg tataggtagc aaa
//
LOCUS       DADLSB010000293         2768 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.294, whole genome shotgun sequence.
ACCESSION   DADLSB010000293 DADLSB010000000
VERSION     DADLSB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.294"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            37..393
                     /locus_tag="JN331_004215"
     CDS             37..393
                     /locus_tag="JN331_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HAY7262148.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            493..1707
                     /locus_tag="JN331_004216"
     CDS             493..1707
                     /locus_tag="JN331_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7262149.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            1934..>2768
                     /locus_tag="JN331_004217"
     CDS             1934..>2768
                     /locus_tag="JN331_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7262150.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VR"
BASE COUNT          671 a          614 c          606 g          877 t
ORIGIN      
        1 tatattttat tgtttatttc atgcagccat tacacgatga acgcttatga actccaggca
       61 ctccgccata tttttgccat gactattgat gaatgcgcga cctggattgc ccagaccggt
      121 gacagtgaaa gctggcgtca gtgggaaaat ggcaaatgcg ccattcctga ttgtgtagtc
      181 gagcaactgt tggctatgcg tcagcaacga aaaaaacatc ttcatgccat catagagaaa
      241 ataaataacc gcatcggcaa caacaccatg cgttttttcc ccgacttaac cgcgtttcaa
      301 caagtctacc ctgatggcaa ttttattgac tggaagatct atcaatcggt agccgctgaa
      361 ttatatgcac acgatctgga gcggctttgt taatttttcc acagaaagga attgtcgttg
      421 ttacaacaat aatgaatgga tactgacaca acatcgctac actttttaaa gcacctttgc
      481 taagtagaac ctatgaaaaa tccctattac cctaccgcac ttgggttgta ttttaattac
      541 ctggtgcatg gtatgggcgt cattttgatg agcctgaata tggcctcgct ggagacactt
      601 tggcagacta atgccgcggg tgtctcgata gttatctcat cgctgggcat tggtcgatta
      661 agtgtcttgc tttttgcagg attattatcc gatcgctttg gtcgccgccc ttttatcatg
      721 ctcgggatgt gctgctatat ggccttcttt tttggcatcc tgcagaccaa taacatcatt
      781 atcgcttatg tttttggctt tctggcggga atggcaaaca gttttctcga tgcaggcact
      841 tatcccagtt tgatggaagc ttttccacgc tcacctggga cagccaatat tttaattaaa
      901 gcatttgttt ccagcggaca atttttatta ccgctaatca ttagcctgtt agtgtgggct
      961 gaactgtggt tcggttggtc ctttatgatt gctgcaggca ttatgtttat taacgctctg
     1021 tttttatacc gttgtacgtt cccaccccat cctggtcgtc gcttacctgt cataaagaaa
     1081 accaccagct ctacggaaca tcgctgttca attatcgatt tagccagtta taccttatac
     1141 ggctatatct caatggcaac gttttatctg gttagccagt ggctggcaca gtacggacaa
     1201 tttgttgcag gcatgtcata cactatgtcg atcaaactac tcagtatcta caccgtgggt
     1261 tcgctgcttt gtgtatttat taccgctcca ctcattcgta ataccgttcg cccaacaaca
     1321 ttactgatgc tgtacacctt tatctcattt attgctctgt ttaccgtctg cctgcatccc
     1381 acattttatg tggtgataat atttgctttt gtcattggtt ttacctctgc tggaggtgtt
     1441 gtgcaaattg gcctgacgtt aatggctgaa cgtttccctt acgctaaagg taaagctaca
     1501 gggatctatt acagtgcggg cagtattgcg acctttacta ttccgttgat tacggctcat
     1561 ctgtcccaaa gaagtattgc cgatattatg tggttcgata ccgccatcgc tgccatcggt
     1621 tttttactgg cactgtttat cggcttacgc agccgcaaaa aaacgcggca tcactcgcta
     1681 aaggaaaatg tcgctccggg tgggtaatgc aatattcttt tcaggtcatg caagatctta
     1741 cggataaata actctttctg cgctaactaa ggaaaatcgc gatcaaaaac aaactatgac
     1801 atgcaatatt cctggaaaca taaactttat gccatgtacc cagggaaaat catcttcagt
     1861 atagtaatta tgtaaaccgt cggagaacaa tacgtacggt aacgaaatta tctttcagca
     1921 aggagctgtg aaaatgtctc aaaataaggc tttcagcacg ccatttatcc tggctgttct
     1981 ttgtatttac ttcagctatt tcctgcacgg cattagtgtt attacgcttg cccaaaatat
     2041 gacatctctg gcagaaaagt tttccactga caacgcgggc attgcctatt taatttccgg
     2101 tatcggtttg gggcgattga tcagtatttt attcttcggt gtgatctccg ataagtttgg
     2161 tcgtcgggcg gtgatattaa tggcagtaat aatgtatctg ctattcttct ttggtattcc
     2221 cgcttgcccg aatttaactc tcgcctacgg tctggcagtg tgcgtaggta tcgctaactc
     2281 agcgctggat acgggtggct accccgcgct catggaatgc tttccgaaag cctctggttc
     2341 ggcggtcata ctggttaaag cgatggtgtc atttgggcaa atgttctacc caatgctggt
     2401 gagctatatg ttgctcaata atatctggta cggctatggg ctgattattc cgggtattct
     2461 gtttgtactg atcacgctga tgctgttgaa aagcaaattc cccagccagt tggtggacgc
     2521 cagcgtagct aatgaattac cgcaaatgaa cagcaaaccg ttagtctggc tggaaggtgt
     2581 ttcatcggta ctgttcggtg tagccgcatt ctcgaccttt tatgtgattg tggtgtggat
     2641 gcccaaatat gcgatggctt ttgctggtat gtcagaagct gaggcattaa aaaccatctc
     2701 ttattacagt atgggctcgt tggtctgtgt ctttattttt gccgcactac tgaaaaaaat
     2761 ggtccggc
//
LOCUS       DADLSB010000294         2762 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.295, whole genome shotgun sequence.
ACCESSION   DADLSB010000294 DADLSB010000000
VERSION     DADLSB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.295"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..711
                     /gene="nikC"
                     /locus_tag="JN331_004218"
     CDS             <1..711
                     /gene="nikC"
                     /locus_tag="JN331_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY7262151.1"
                     /translation="PSCLLSPDAQHWLGTDHLGRDIFSRLMAATRMSLGSVMACLLLV
                     LTLGLVIGGSAGLIGGRVDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIAL
                     SHWAWYSRMVRSLVISLRQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIG
                     HMMLHVAGMSFLGLGVTAPTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLV
                     GDALRDHLDPHLVTEHAH"
     gene            711..1475
                     /gene="nikD"
                     /locus_tag="JN331_004219"
     CDS             711..1475
                     /gene="nikD"
                     /locus_tag="JN331_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY7262152.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            1472..2278
                     /gene="nikE"
                     /locus_tag="JN331_004220"
     CDS             1472..2278
                     /gene="nikE"
                     /locus_tag="JN331_004220"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY7262153.1"
                     /translation="MSLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            2284..2685
                     /gene="nikR"
                     /locus_tag="JN331_004221"
     CDS             2284..2685
                     /gene="nikR"
                     /locus_tag="JN331_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY7262154.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
BASE COUNT          580 a          782 c          797 g          603 t
ORIGIN      
        1 ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg gcaccgatca cttaggtcgc
       61 gatattttct cgcggctgat ggcagcgacc cgcatgtcgc tcggttcggt aatggcctgc
      121 ctgctgctgg tgctgacatt agggctggtt attggcggca gcgccgggtt gattggcggg
      181 cgcgttgatc aggccaccat gcgcgtcgcc gatatgttta tgaccttccc gacctcgatt
      241 ctgtcgttct ttatggttgg cgtgctcggc accgggctga ccaacgtaat tatcgccatc
      301 gccctgtcgc actgggcgtg gtattcacgc atggtgcgca gcttggtgat ttcactacgc
      361 caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg gccatgtgcg ggtgtttgtc
      421 gatcatctgg caggcgcggt gatcccttcg ctgctggtgc tggcaacgct ggatatcggc
      481 catatgatgc tgcacgtcgc ggggatgtct ttccttggcc tcggtgtgac cgcgccgacc
      541 gccgaatggg gcgtgatgat taacgacgcg cgccagtata tctggaccca gccgctgcaa
      601 atgttctggc cggggctggc gctgtttatc agcgtgatgg cctttaacct ggtgggtgac
      661 gcactgcgcg atcatctgga tcctcatctg gtgacggagc acgcacacta atgccgcaac
      721 agattgaact acgtaatatc gcgctacagg ccgcgcagcc gctggtacac ggtgtatcgt
      781 taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg tagcggcagc gggaaatcat
      841 taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt tcgccagacg gcgggggaaa
      901 ttttagccga tggcaaaccg gtttcgcctt gcgccctgcg cggcatcaaa attgccacca
      961 tcatgcagaa cccgcgcagc gcctttaatc cactgcacac catgcacacc cacgcgcggg
     1021 aaacctgcct ggcgttaggg aaacccgccg atgacgctac gcttaccgct gccatagaag
     1081 cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta cccgttcgag atgagcggcg
     1141 gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg tgaatcaccg tttatcatcg
     1201 ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc gcgcatcctc gatctgctgg
     1261 aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt cacccatgat atgggcgttg
     1321 tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg taaaattgtc gaacagggcg
     1381 atgtagaaac gctgtttaac gcccccaaac atacggtgac gcgcagcctg gtttccgctc
     1441 atctcgccct ctacggtatg gagctggcat catgagctta cttaacgtct gcggcctttc
     1501 ccatcactat gcccacggtg gatttagcgg aaaacatcaa catcaggcgg tgctgaataa
     1561 cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg ctgggacgca gcggctgcgg
     1621 aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct ccctcacaag ggaatattag
     1681 ctggcgcggc gaatcgctgg cgaaacttaa tcgcgcccaa cgtaaagcct tccgccgtga
     1741 tattcagatg gtgtttcagg actccatcag tgccgtgaat ccgcgcaaaa ccgtgcgcga
     1801 gatcctgcgt gaaccgatgc gccacctgct atcactgaaa aaatccgaac aactggcgcg
     1861 cgccagcgaa atgctgaagg cggtcgatct cgatgacagc gttctcgaca aacgcccacc
     1921 gcagttaagc ggcggccagc tccagctcgt ctgcctggca cgcgcgctgg cggtcgaacc
     1981 gaaattgctg attctggatg aagccgtttc taaccttgat ctcgtgttac aggcgggcgt
     2041 cattcgcctg ctgaaaaagc tacaacaaca gtttggcact gcctgcctgt tcatcactca
     2101 tgacttacgc ctggtggaac gcttttgcca gcgagtgatg gtgatggaca acggacaaat
     2161 cgtcgaaacc caggcggtgg gagataaatt aaccttttcc tctgacgccg gacgtgtgct
     2221 acaaaacgcg gtattacccg cattccccgt gcgccgtcgc acctcagaaa aggtttaacg
     2281 ccaatgcaac gagtcaccat cacgcttgat gacgatttac tggagacgct ggacagcctg
     2341 agccagcgtc gtggttataa caaccgttcc gaagctatcc gcgatattct gcgtagtgcc
     2401 ctggcgcagg aagctaccca gcagcacggc acacaaggtt tcgcggtgct gtcgtatgtg
     2461 tatgaacacg aaaaacgcga cttagccagc cgtattgttt ccacccagca tcatcaccac
     2521 gacctctccg tcgccacgct acatgtgcac atcaaccacg acgactgtct ggaaatcgcc
     2581 gtgttgaaag gtgacatggg tgacgtgcag cattttgccg atgacgttat cgcccagcgc
     2641 ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag attgagttat ttctaaaaca
     2701 aggaaagaaa agatattaac tgaacttaat ttataaaatt aagttcagtt gcatagatag
     2761 at
//
LOCUS       DADLSB010000295         2702 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.296, whole genome shotgun sequence.
ACCESSION   DADLSB010000295 DADLSB010000000
VERSION     DADLSB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.296"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..407
                     /locus_tag="JN331_004222"
     CDS             <1..407
                     /locus_tag="JN331_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262155.1"
                     /translation="ADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVED
                     VDNADINQRLLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAAL
                     VYKIFCISESNDARECAAPGAVACRDCGETNA"
     gene            583..762
                     /locus_tag="JN331_004223"
     CDS             583..762
                     /locus_tag="JN331_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7262156.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREVEVGHEH"
     gene            752..1693
                     /locus_tag="JN331_004224"
     CDS             752..1693
                     /locus_tag="JN331_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7262157.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTAEQDFLTRHPDAVVFSPKKRQWGTQNDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPEGFRG"
     gene            1788..2441
                     /locus_tag="JN331_004225"
     CDS             1788..2441
                     /locus_tag="JN331_004225"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HAY7262158.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDSDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            2438..>2702
                     /locus_tag="JN331_004226"
     CDS             2438..>2702
                     /locus_tag="JN331_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7262159.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISEAGQKFQSAACAAI
                     IEQLRRLPKPTSAHASVEIVLFPPDNRTRDLDNYNKALFDALTH"
BASE COUNT          678 a          652 c          764 g          608 t
ORIGIN      
        1 gtgccgatgg cgatcagatt ttcccgctgg gatgggcaat gattttgcaa cgtgctggtg
       61 gaactcactt cattgctgac gctgtggcgc agtctgcaaa tggcgtcttt gtgtctcttc
      121 ctgacgtcga ggatgtggac aacgccgata ttaaccagcg cctgctggaa gtcattgaac
      181 agatcggcag ttattcaaaa cagattcgtt cagcaattga agacggtgta gtggaaccgc
      241 atgagaagac agcaattaac gacgagctgt acctctcaat ttcgaagctg caggagcatg
      301 cagcactggt ctacaaaatt ttttgcattt cagaaagtaa tgacgcccgc gagtgtgcag
      361 ctccgggcgc cgtggcgtgt cgtgactgtg gagaaactaa cgcatgaaca gtttaacaac
      421 acactaccgt cgctcgcaac tgattgcgct tcctgtaccg ggtggaaaag cgaaggtgga
      481 gtattgctat gcagtaaatg taccaggtga cagggaaatt gtaacccaca gctttgcaga
      541 gtgggctgtg ggtgatttca accggcagaa ggagacagtc ctttgcgaca agttaaccgc
      601 tggttcaaag atcactacgg agtgcccgtc agagtcattc gttgggagcc ggaaacacaa
      661 cgggttatct acctccgcga aggctatgag catgagtgct tcagcccgct cgaacagttt
      721 cgtcgtaaat tcagggaagt agaggtcggt catgagcact aaattaaccg gctatgtatg
      781 ggatggttgc gctgcatcag gcatgaaatt atccagcgtg gcaattatgg cccgcctggc
      841 tgatttcagt aatgacgaag gtgtgtgctg gccatcaatt gaaaccattg cccgtcagat
      901 tggcgcgggg atgagtaccg tcagaacggc tatcgcacgg ctggaagcag aaggctggtt
      961 aacgcgtaag gcgcgtcgcc agggtaaccg caatgcgtcg aatgtttatc agcttaacgt
     1021 tgcgaagctt caggcagcgg cattttctca actgtcagat tctgacccgt caaaatctga
     1081 cgcatcaaaa tctgacccgt caaaatttga tgcgtcgaaa tctggcaaaa aagcgggttt
     1141 tcacccgtca gaatctggcg gggatccgtc agtaaaatca aaacatgatc cgtcagataa
     1201 aaaaccttct cgtccggacg cttcgcaacc ggacacgcag acggctgaac aggatttttt
     1261 aactcgccat cctgatgcgg ttgtattcag ccctaaaaag cgccagtggg ggacgcagaa
     1321 tgatttgacc tgcgcacagt ggctctggaa aaaaatcatc gccctgtacg agcaggctgc
     1381 cgaatgtgac ggcgaggtgg ttcgtcccaa agaaccgaac tggacagcct gggcaaacga
     1441 aattcgcctg atgtgtgtgc aggatggtcg tactcacaaa caaatctgcg agatgtacag
     1501 ccgcgtcagc cgcgatccgt tctggtgccg taacgtgctc agcccgtcga agctgcggga
     1561 aaaatgggat gagctttccc tgcgcttatc gccgtccgtc agcacgtaca cagaaaaacg
     1621 cgaagacccg tacttcaaag ccagttacga caacgtggac tacagccaga tcccggaagg
     1681 attcaggggg tgatcatgag tcttttgaat gaagttcaga aattcattga agcccatccg
     1741 gggtgtactt ccggagacat tgcggatgct ttttacgtgg gggcttaatg agtaataaat
     1801 attgccaggc gctggtagaa ctgcggaaca aaccagccca tgaactgaag gaagtgggcg
     1861 atcagtggcg cacgccggac aacattttct ggggaattaa caccttgttt ggtccgtttg
     1921 ttctggatct gttcactgac agtgataacg ccaaatgtgc cgcgtattac acggcggaag
     1981 acaacgcgct ggcgcatgac tggtcagaac gtcttgcgga gcttaaaggt gctgcctttg
     2041 gcaatccccc gtacagccgc gccagtcagc atgaggggca atacatcacc ggcatgcgtt
     2101 acatcatgaa gcatgccagt gccatgcgtg ataaaggcgg gcgctatgtt ttcctgatca
     2161 aagctgccac cagcgaagtg tggtggccgg aagatgcaga tcatattgct tttattcgcg
     2221 ggcgtattgg ttttgaactg cctgcctggt ttatcccgaa agacgaaaag caggtgccaa
     2281 caggtgcttt cttcgctggt gctattgctg ttttcgacaa gacctggaag ggaccggcaa
     2341 tcagctacat cgggcgcgat gaacttgagg catgtggtga ggcatttctg gcgcaggttc
     2401 gccagcaggc ggaaaaactg gtcagggaga tggtggcatg aagctaatac tgccttttcc
     2461 gcccagcgtg aacacgtact ggcgacaccc caacaaaggg gcatttgctg gtaagagcct
     2521 gataagcgag gcggggcaaa aatttcagag cgcggcgtgt gccgccatca ttgagcagtt
     2581 acgtcgtctg ccgaaaccaa cgtcggcaca tgcttcagtg gagatcgtgt tgtttcctcc
     2641 ggataacagg acccgcgatc tggacaacta taacaaggcg ctgtttgacg ccctgaccca
     2701 cg
//
LOCUS       DADLSB010000296         2697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.297, whole genome shotgun sequence.
ACCESSION   DADLSB010000296 DADLSB010000000
VERSION     DADLSB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.297"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="JN331_004227"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="JN331_004227"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY7262160.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2096)
                     /locus_tag="JN331_004228"
     CDS             complement(1725..2096)
                     /locus_tag="JN331_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7262161.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2463)
                     /locus_tag="JN331_004229"
     CDS             complement(2119..2463)
                     /locus_tag="JN331_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7262162.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT          668 a          648 c          672 g          709 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggaactgga cgatcttatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccat ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacaat ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctgatccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtga
//
LOCUS       DADLSB010000297         2640 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.298, whole genome shotgun sequence.
ACCESSION   DADLSB010000297 DADLSB010000000
VERSION     DADLSB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.298"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..246
                     /locus_tag="JN331_004230"
     CDS             <1..246
                     /locus_tag="JN331_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262163.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JN331_004231"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JN331_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY7262164.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JN331_004232"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JN331_004232"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7262165.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..>2640
                     /gene="mdfA"
                     /locus_tag="JN331_004233"
     CDS             1990..>2640
                     /gene="mdfA"
                     /locus_tag="JN331_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY7262166.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGSLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGIILCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLK"
BASE COUNT          670 a          645 c          682 g          643 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtcgga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggtc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 atcctctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccacg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
//
LOCUS       DADLSB010000298         2532 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.299, whole genome shotgun sequence.
ACCESSION   DADLSB010000298 DADLSB010000000
VERSION     DADLSB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.299"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            109..288
                     /locus_tag="JN331_004234"
     CDS             109..288
                     /locus_tag="JN331_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262167.1"
                     /translation="MTLVSVLATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDF
                     MMACDMYFRWGHFNK"
     gene            complement(481..568)
                     /locus_tag="JN331_004235"
     tRNA            complement(481..568)
                     /locus_tag="JN331_004235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(532..534),aa:Ser,seq:gga)
     gene            802..1740
                     /gene="ghrA"
                     /locus_tag="JN331_004236"
     CDS             802..1740
                     /gene="ghrA"
                     /locus_tag="JN331_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HAY7262168.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGCKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            1795..2532
                     /locus_tag="JN331_004237"
     CDS             1795..2532
                     /locus_tag="JN331_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY7262169.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHLVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
BASE COUNT          613 a          575 c          643 g          701 t
ORIGIN      
        1 gtttacgggg ttttttactg gttgatactg tttttgtcat gtgagtcacc tctgactgag
       61 agtttactca cttagccgcg tgtccactat tgctgggtaa gatcatggat tacgctggtg
      121 tcggtattgg caacgcttgt ggaatggcgg atttggtttg aagcctattt tttactctgc
      181 tatgtaccgt gcttaattta ctattttttc cattgtcgat tcctctggca taacgatttt
      241 atgatggcat gtgacatgta tttccgttgg gggcatttta ataagtgagg aagttacagg
      301 aagtgaccag ataacgcata tatgttctgt actatcttgc gcattttgat tgttgactga
      361 gtaaccagac agttgatgtg cacaatttcc cctcgcccta acagacgtgg gcgggggcac
      421 ccggtgtgta caatatacga aaaaaaagcc cgtactttcg tacgcgctct tctttaaata
      481 tggcggtgag ggggggattc gaacccccga tacgttgccg tatacacact ttccaggcgt
      541 gctccttcag ccactcggac acctcaccaa attgtcgttc ctgtcttgct ggaacgggcg
      601 ctaatttagg gaaatcatga cctgaggtca acaaactttt tgaaaaaatc gcgcgtttat
      661 tcaaacttca atcaatgtgt ggttttaata agcgaaatct gctttttttg ccaccgacca
      721 cggatttgtt atgctggtgg cctttgtaga tcataacgat aagtgcgaat aaatttcgca
      781 caacgctttt cgggagtcag tatggatatc atcttttatc acccaacgtt cgatacccaa
      841 tggtggattg aggcactgcg caaagctatt cctcaggcaa gagtcagggc atggaaaagc
      901 ggagataatg actctgctga ttatgcttta gtctggcatc ctcctgttga aatgctggca
      961 gggcgcgatc ttaaagcggt gttcgcactc ggggccggtg ttgattctat tttgagcaag
     1021 ctacaggcac accctgaaat gctgaaccct tctgttccac tttttcgcct ggaagatacc
     1081 ggcatgggcg agcaaatgca ggaatatgct gtcagtcagg tgctgcattg gtttcgacgt
     1141 tttgacgatt atcgcatcca gcaaaatagt tcgcattggc aaccgctgcc tgaatatcat
     1201 cgggaagatt ttaccatcgg cattttgggc gcaggcgtac tgggctgtaa agttgctcag
     1261 agtctgcaaa cctggcgctt tccgctgcgt tgctggagtc gaacccgtaa atcgtggcct
     1321 ggcgtgcaaa gctttgccgg acgggaagaa ctgtctgcat ttctgagcca atgtcgggta
     1381 ttgattaatt tgttaccgaa tacccctgaa accgtcggca ttattaatca acaattactc
     1441 gaaaaattac ctgatggcgc gtatctcctc aacctggcgc gtggtgttca tgttgtggaa
     1501 gatgacctgc tcgcggcgct ggatagcggg aaagttaaag gcgcaatgct ggatgttttt
     1561 aatcgtgaac ccttaccgcc tgaaagtccg ctctggcaac atccacgcgt gacgataaca
     1621 ccacatgtcg ccgcgattac ccgtcccgct gaagctgtgg agtacatttc tcgcactatt
     1681 gcccagctcg aaaaagggga gagggtctgc gggcaagtcg accgcgcacg cggctactaa
     1741 taaagcatca ggattcctgc tatccttggc gggaattgaa tacaggagag agttatgtat
     1801 cccgtcgacc ttcatatgca taccgttgcc agcacacatg catatagcac attgagtgat
     1861 tacattgccc aggccaaaca aaagggcatt aaactttttg cgatcaccga tcatggcccg
     1921 gatatggaag atgcgccgca tcattggcac ttcattaaca tgcgtatctg gccgcgagtg
     1981 gttgatgggg tagggatcct gcgcggcatt gaagctaaca ttaaaaatgt tgatggtgaa
     2041 attgactgca gcggtaaaat gtttgactcg ctggatctga ttattgccgg ttttcatgag
     2101 ccggtttttg cgccacatga caaagcgacc aatacacaag cgatgatcgc cactatcgcc
     2161 agcggcaatg tgcatataat aagccatccc ggaaatccga aatatgaaat agatgttaaa
     2221 gccgttgctg aagcagccgc gaaacatctg gtggcgctgg aaatcaataa ttcctcattt
     2281 ttacactcac gtaagggcag tgaagacaac tgtcgtgcgg tagctgcagc ggtacgtgat
     2341 gctggaggtt gggtggcatt aggctcggat tctcacaccg cgtttaccat gggggaattt
     2401 gaagagtgtc ttaaaatcct cgacgcggta gattttccgc cagagcgcat tttgaatgtt
     2461 tctccgcgcc gcttactgaa cttccttgaa tctcgcggta tggcaccgat tgcggaattt
     2521 gcagaccttt aa
//
LOCUS       DADLSB010000299         2513 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.300, whole genome shotgun sequence.
ACCESSION   DADLSB010000299 DADLSB010000000
VERSION     DADLSB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.300"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..653)
                     /locus_tag="JN331_004238"
     CDS             complement(<1..653)
                     /locus_tag="JN331_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAY7262170.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFY"
     gene            complement(805..1278)
                     /locus_tag="JN331_004239"
     CDS             complement(805..1278)
                     /locus_tag="JN331_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAY7262171.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            1434..2447
                     /gene="intI1"
                     /locus_tag="JN331_004240"
     CDS             1434..2447
                     /gene="intI1"
                     /locus_tag="JN331_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7262172.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
BASE COUNT          541 a          700 c          680 g          592 t
ORIGIN      
        1 taataaaatg ctgaggcctg gcctttgcgt agtgcacgca tcacctcaat acctttgatg
       61 gtggcgtaag ccgtcttcat ggatttaaat cccagcgtgg cgccgattat ccgtttcagt
      121 ttgccatgat cgcattcaat cacgttgttc cggtacttaa tctgtcggtg ttcaacgtca
      181 gacgggcacc ggccttcgcg tttgagcaga gcaagcgcgc gaccataggc gggcgcttta
      241 tccgtgttga tgaatcgcgg gatctgccac ttcttcacgt tgttgaggat tttacccaga
      301 aaccggtatg cagctttgct gttacgacgg gaggagagat aaaaatcgac agtgcggccc
      361 cggctgtcga cggcccggta cagatacgcc cagcggccat tgaccttcac gtaggtttca
      421 tccatgtgcc acgggcaaag atcggaaggg ttacgccagt accagcgcag ccgtttttcc
      481 atttcaggcg cataacgctg aacccagcgg taaatcgtgg agtgatcgac attcactccg
      541 cgttcagcca gcatctcctg cagctcacgg taactgatgc cgtatttgca gtaccagcgt
      601 acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc
      661 tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac agtgcccaat
      721 attcacataa cgtcgctatc aatcgcggaa aaggcgtagt gcgcgaagcg aacgaagctt
      781 tttgccgtcg attgcatagc tttgttaacc ctttttccaa atttgatagc aatagttaat
      841 gtttgaagta aagtgttgct caaaaacaac ttcgaaggta tttggaatac tcgggaagaa
      901 aacatccccc tctggctcga tgtcgatcgt cgataagtgg agcgtagagg ccatgggtaa
      961 tgtttctcgg taaatttctc cgccaccaga cactataacg tgaccggtga attcagctag
     1021 cctgtccatg gcctcttcga ttgactgaaa tacaactaca ttgtcatcat ttgatgtcca
     1081 acctgagcgg gtaacgaccg cgtatttcct attggggagt gcgcccatag attcaaacgt
     1141 cttgcgaccc accagaagcc actgattgta ggtcaatgct ttaaaaagta gctgctcccc
     1201 tttcgcggac cagggtatgt ctggaccgca accaatcacg ccgtttttcg ctttcgcagc
     1261 catcaatgat actttcaagg ttctcatcct gggttaactt tgttttaggg cgactgccct
     1321 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
     1381 gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa gccatgaaaa
     1441 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
     1501 gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtccac tgggttcgtg
     1561 ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg
     1621 catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat
     1681 tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg
     1741 agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg
     1801 ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa
     1861 cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg
     1921 gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga
     1981 gcttggcacc cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc
     2041 aggccgaggg ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg
     2101 ccgggcattc ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac
     2161 ggagcggtgt cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac
     2221 gtgccgtaga acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt
     2281 tcgcgacggc cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc
     2341 attccgacgt ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag
     2401 tgcgctcacc gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc
     2461 aatcctggcg gattcactac ccctgcgcga aggccatcgg tgccgcatcg aac
//
LOCUS       DADLSB010000300         2439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.303, whole genome shotgun sequence.
ACCESSION   DADLSB010000300 DADLSB010000000
VERSION     DADLSB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.303"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..565
                     /locus_tag="JN331_004241"
     CDS             <1..565
                     /locus_tag="JN331_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7262173.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="JN331_004242"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="JN331_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY7262174.1"
                     /translation="MEHSRIKKRNVALIEKCIMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JN331_004243"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JN331_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY7262175.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="JN331_004244"
     CDS             2167..2403
                     /locus_tag="JN331_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY7262176.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          536 c          625 g          650 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcatcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgc ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccggtttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DADLSB010000301         2435 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.304, whole genome shotgun sequence.
ACCESSION   DADLSB010000301 DADLSB010000000
VERSION     DADLSB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.304"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..310
                     /locus_tag="JN331_004245"
                     /pseudo
     CDS             1..310
                     /locus_tag="JN331_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108985.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            336..644
                     /locus_tag="JN331_004246"
     CDS             336..644
                     /locus_tag="JN331_004246"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262177.1"
                     /translation="MNVEKEIYTGLYLFGNMKLNTSCGGVSAVIKVPTYNSNVLFFIN
                     DSHVSEEDSMENAKCYKFNLEELESIQKAIERALNDIRNLESLSRRNLLKDKAYRNPI
                     "
     gene            815..895
                     /locus_tag="JN331_004247"
     CDS             815..895
                     /locus_tag="JN331_004247"
                     /inference="COORDINATES: protein motif:HMM:NF015490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262178.1"
                     /translation="MWYYYTDLTGTVQEVTASDGTLVWAG"
     gene            883..1779
                     /locus_tag="JN331_004248"
     CDS             883..1779
                     /locus_tag="JN331_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY7262179.1"
                     /translation="MGWLTSRTGQMYDHDHYYYDKAGNLLTDEYQSAVMDNRLPGYGR
                     DRYRYNEWGELTERRDQQLEWNAQGQLTRVISSNSDTRYQYDALGRCISKATYGRHTD
                     RGERSRTTFVWEGFRLLQETTWQGKRTYLYDAEQPYTPVAAITGRGESQKIWYYHTDL
                     TGTVHEVTAPDGTLVWAGYQAGFGENRGDISNSGAYFEQPQRLPGQYFDEETGLHYNL
                     FRYYAPECGRFVSQDPIGLNGGLNLYAYAPNPLGWIDPLGLNSLGIDSNNIFRGDGNY
                     KGGGIGQPKSGTISAQDIIDHL"
     gene            1879..2082
                     /locus_tag="JN331_004249"
     CDS             1879..2082
                     /locus_tag="JN331_004249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262180.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            2100..2420
                     /locus_tag="JN331_004250"
     CDS             2100..2420
                     /locus_tag="JN331_004250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262181.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
BASE COUNT          766 a          436 c          659 g          574 t
ORIGIN      
        1 ttatggttta ctcaaaacac aatatcagac gtgtttacac aaggaaaatg ggccgggaga
       61 tcggttgaag aagcgattca tgaggctagt gtcacggggc ggcttcctga tgggctgagt
      121 attagttatg aaaagataat tctgccaagc ggggaggagg tttgggcgac tctaaataat
      181 cgtacgcttt atgtggccca acaggcaaat gttacaatta acccagtaga taaaaatgga
      241 aaaggattaa atcagttgaa taaacttttg gatggaaggg cacccactcc agaacaaccg
      301 gatgttttaa taaaacactg ctaacaggat gcgttatgaa tgtagaaaaa gaaatttata
      361 cagggcttta tttgtttgga aatatgaaat tgaacacatc atgcggaggt gtatctgcag
      421 ttatcaaagt accgacatat aatagtaatg ttttattttt tataaatgat tcacatgtaa
      481 gtgaagaaga tagcatggaa aatgcgaaat gttataaatt taacttagaa gaattagaaa
      541 gtatacaaaa ggcgattgaa agagctttaa atgacatacg aaatctagag tctttatcac
      601 gcaggaattt gcttaaggat aaagcttata gaaaccccat ataatccagc actctgaagt
      661 aatgcgggcg cacaacttgc cctcatatag acgcagggaa tacaagctgt tgctggaaat
      721 accatagcag agcgagcgca ggatgtatct ttacgactcg gagcaggcgt acaccccggt
      781 ggcgagcgtg acggggcgct gagaaagccg aaaaatctgg tattactaca cagacctgac
      841 aggcacggtg caggaggtga ccgcgtcgga cgggacgctg gtgtgggctg gctgaccagc
      901 cgcacgggtc agatgtacga ccatgaccat tattattacg acaaagcggg caacctgctg
      961 acggacgagt atcagagcgc ggtgatggat aaccgcctgc cgggctacgg gcgtgaccgt
     1021 taccgctata acgaatgggg cgaactgaca gagcgcaggg accagcaact ggagtggaac
     1081 gcgcagggcc agttaacgcg ggtcatcagc agcaacagcg atacgcggta tcagtatgat
     1141 gcgctgggca ggtgcatcag caaggcgaca tatggccgcc atacggaccg gggagaacga
     1201 agccggacga cgtttgtgtg ggaagggttc cggctgttgc aggagacaac gtggcaggga
     1261 aagcggacat acctgtacga cgcggaacag ccgtacacgc cggtggccgc catcacgggg
     1321 cgcggagaga gccagaaaat ctggtattac cacacggacc tgacgggcac ggtacacgag
     1381 gtaaccgcgc cggacgggac gctggtgtgg gcgggctacc aggcgggatt tggtgaaaac
     1441 cggggcgaca tcagcaacag cggggcgtac tttgagcagc cgcagcggct gccggggcag
     1501 tactttgacg aagaaacagg gctgcattac aatctgttca ggtattatgc gccggagtgc
     1561 gggaggttcg taagccagga cccgatagga ttaaacggtg ggttgaactt atatgcttat
     1621 gcgcctaatc cgttaggttg gatcgatccg ctaggactga attctttagg aatagattca
     1681 aataatatat ttagaggaga tggtaactat aaaggtggcg gaattggaca gccaaagagt
     1741 ggcacaatat cagcacagga cattattgat catctctaag aaaaaggtga tgggcgttta
     1801 acatctttta gtactaaaaa aacatctagt tccaactctc gtggtgcaga ttttttctca
     1861 aaaaaccatg tcttcaaagt ggcagtttct gatctcgatg atttagttaa aaataatcag
     1921 atcactgttt taacacaaga aaaagtaaaa gcaatatttg gtttagaatc aagcaagaaa
     1981 attagaaaag aacttggtaa tattcttcag aatatggcta ggaataatga ggtgttagtt
     2041 catgggcaaa ttcttggaaa taaagttagc cgatgtaatt agttagtgga gaggaagttg
     2101 tggacgaaat tagaataaga attgctggta atacggctgc gccttgtttc catgtgctaa
     2161 aaaataaggg atataaagta acaatggaaa ctcatgcatt atctgacgat tgtttagatt
     2221 gtgtatatga ttggtatgcg gaaaaaaatg gacaattttt tagtgcaacg agtccagaag
     2281 agttacttgg attagttgca atgtgggaag ttcgtggtaa tgactggaga gctaatttga
     2341 atgagataaa tacttatcaa aaagccatag aagaatcccc gttttttgat aaaaacggca
     2401 atgaaataga gtctgactaa attttattga tatag
//
LOCUS       DADLSB010000302         2422 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.305, whole genome shotgun sequence.
ACCESSION   DADLSB010000302 DADLSB010000000
VERSION     DADLSB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.305"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..420
                     /locus_tag="JN331_004251"
     CDS             <1..420
                     /locus_tag="JN331_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7262182.1"
                     /translation="LTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLA
                     ITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEI
                     GFSSGFYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            463..966
                     /gene="yeaK"
                     /locus_tag="JN331_004252"
     CDS             463..966
                     /gene="yeaK"
                     /locus_tag="JN331_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY7262183.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(967..1071)
                     /locus_tag="JN331_004253"
     CDS             complement(967..1071)
                     /locus_tag="JN331_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262184.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            1241..1687
                     /locus_tag="JN331_004254"
     CDS             1241..1687
                     /locus_tag="JN331_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY7262185.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPITSGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1644..>2422)
                     /locus_tag="JN331_004255"
     CDS             complement(1644..>2422)
                     /locus_tag="JN331_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7262186.1"
                     /translation="HEAAVAFCIHAGTDELTSPVHQHRKGQLILALHGAITCTEENAL
                     WMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTCCTLKISPLCRELILT
                     LANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKIRTMVEMMAKGPVEWG
                     SLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQGLVKGDTVQKVAHTLG
                     YDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
BASE COUNT          598 a          597 c          591 g          636 t
ORIGIN      
        1 ttaacccctg aactggagca gcggttgcag aaactggtgc aatccggttc ttcggtgatg
       61 tttattgcaa ttgacatgga caagttaaag caaataaatg acaccctcgg tcatcaggag
      121 ggggatttag cgattacgtt attagctcag gcgattaaac aatcgattcg taaaagtgat
      181 tatgccatcc gactcggtgg cgatgaattc tgcatcattc ttgtcgattc gacgccgcaa
      241 attgcagcac aactgcctga acgtatcgaa aaacgtctgc aacatatcgc gccgcagaaa
      301 gagatcggct tctcttccgg tttttatgcg atgaaagaaa acgatacgtt acatgatgcg
      361 tataaagctt ccgatgagcg tttatatgtc aataagcaga acaaaaacag ccgttcatga
      421 taaccttctg tggttgtttg cttgtaatct caggagcgtg aaatgactga aatggctaaa
      481 ggaagcgtga cgcatcagca gttaatcgct ttattatcac aagaaggtgc taacttccgc
      541 gtcgttaccc atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg tggcaccgct
      601 ctaggccagg gtgctaaggc actggtatgt aaagtcaaag gcaatggcgt aaatcaacat
      661 gttctggcaa tcctcgcagc cgatcaacag gccgatctga gccaactcgc cagccatata
      721 ggcggattac gcgcttcgtt ggccagcccg gcggaagttg atgaactcac aggctgtgtc
      781 ttcggcgcga tccccccttt cagcttccat ccaaaactca aactggttgc cgaccctcta
      841 ctcttcgaac gatttgatga aatcgccttc aatgcaggga tgctggataa atccgttatt
      901 ttgaaaaccg cggattattt gcgcattgcg caaccagaac tggtcaattt ccgccgcact
      961 gcgtagctag cctgtccgtt caataagcag aacggacaaa accagcacaa ccgcaatagc
     1021 aaaaaacgat gatgtgataa tcagtgtctc tacgaacatt tgatcgttca tagcatgccc
     1081 ccgggagata gctgttttca cgttattgtt agcgtgcaca aatggcagtt tgatgacagt
     1141 tcgcctgatt ttttatgcaa aaaacgtaaa gattttctac ttccttcctg cagcaagcgt
     1201 aaagtaagca ggcttattat tttttggcaa ggaaaccacg atgtttgatg tcactttgct
     1261 gatcctgctc gggttagctg cgctgggctt tatcagtcat aacaccactg tcgccgtttc
     1321 aattctggtg ttgatcattg tccgcgtcac accgttaagc acctttttcc cgtggattga
     1381 aaaacagggg ctaagtatcg ggataatcat tctgacgatt ggcgttatgg cccccatcac
     1441 cagcgggacg ctaccgccct caaccttgat tcactcgttt ctgaactgga aatcgctggt
     1501 ggcaattgca gtaggcgtta ttgtctcctg gctgggtggg cgcggcgtga cgttaatggg
     1561 cagccagccg caactggttg ctgggctgct ggtgggcact gttttaggcg tagcgctgtt
     1621 tcgcggcgta ccggttggcc cgcttattgc cgcgggtctg gtttcgctga ttgtggggaa
     1681 acagtagtta atcccgcaat atatcggcct ggcgtttgcc ccaggccttt cttaaacata
     1741 gtaataaagg ccgttgttga atcatacccc agcgtatgag caactttctg tacagtatcg
     1801 cctttcacta atccctgaag cgccataatc agctgtaact gctgacgcca ctggcgaaaa
     1861 ctcaaccccg tctctttcac tatcagtcgg gcaagattgc gttcactcat cgcaaaaaag
     1921 ccagcccatt gccccaatga cccccactcg acgggccctt tcgccatcat ctccaccatc
     1981 gtgcggattt taggatgaga agaaacgggc aggtgcagct gttgttgtgg ctgctgaggg
     2041 agttcatcaa acaggacctg aattaatctg cgggtcatcg gctcagcacg ctgagtcgtc
     2101 gtcctgtttg ccagggttaa aatcaattcc cggcataagg gcgatatttt caatgtgcaa
     2161 catgttgtcg gcattgtcac tgcgctgggt tcgataaaca gaaaacagag ttcagcgtta
     2221 gctgtcactt gattgctatg cttcaccccg cccggtatcc aaaccgcata ttgtggcggc
     2281 accatccaca aagcattttc ctccgtacag gtaattgcac catgtaatgc tagtatcagc
     2341 tgtcctttgc ggtgctgatg tacaggactg gttaactcat ccgttccggc gtgaatacaa
     2401 aatgcgaccg cggcttcatg gt
//
LOCUS       DADLSB010000303         2414 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.306, whole genome shotgun sequence.
ACCESSION   DADLSB010000303 DADLSB010000000
VERSION     DADLSB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.306"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            5..1138
                     /gene="blaEC"
                     /locus_tag="JN331_004256"
     CDS             5..1138
                     /gene="blaEC"
                     /locus_tag="JN331_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7262187.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1227..1760
                     /locus_tag="JN331_004257"
     CDS             1227..1760
                     /locus_tag="JN331_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7262188.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1757..2074)
                     /locus_tag="JN331_004258"
     CDS             complement(1757..2074)
                     /locus_tag="JN331_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY7262189.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2250..2396)
                     /gene="ecnB"
                     /locus_tag="JN331_004259"
     CDS             complement(2250..2396)
                     /gene="ecnB"
                     /locus_tag="JN331_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7262190.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT          615 a          634 c          599 g          566 t
ORIGIN      
        1 ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc
       61 tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca
      121 aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg
      181 gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg
      241 ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat
      301 caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg
      361 gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc aggtgccgga
      421 tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc
      481 tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt
      541 gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa
      601 actcaaccat acgtggatta atgtacctcc cccagaagaa aagaattacg cctggggata
      661 tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt
      721 gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttaaccc cccttgatat
      781 caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg
      841 cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc ctgacagcat
      901 cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag cgattaagcc
      961 cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg
     1021 tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa
     1081 ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa
     1141 tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt
     1201 agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag
     1261 ctgcatttct ggtcgttgcc tgtagttctc ctacgccgcc gcgtggcgtg accgtagtga
     1321 ataatttcga cgccaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc
     1381 gttttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc
     1441 tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag agtgaaggga
     1501 aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt
     1561 tctatggcgg ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg
     1621 gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga
     1681 aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg
     1741 tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta
     1801 acgccagact cgccagacgc atcgggttag cggactcacc gagcagcaca atgccggtga
     1861 tggccgcgcc gactgcgcca atacccgtcc acacggcata agccgtccct accggtaacg
     1921 atttcatcgc ccaggcaagt agcgccatac tgactatcat cgccgtcacg gtaataacac
     1981 tcggcgtcaa acggctaaag ccgtgggtat atttcaggcc aacggcccat accacttcca
     2041 gcagaccagc aataactaag ataatccagg acatatcagg ctccggaaat ttggggccgt
     2101 ccccggtgaa aagaggcgtt tgcaggtcgt cctgcaaagc tgatggttga acggatattt
     2161 tgccaggtgg caggggaatt tcaactatta cctttatgac gatcgcagaa aaaacaaacg
     2221 gcacgacact gctgtcgtac cgttattgct tattgctgcg ctttcgttgc tgcgccagaa
     2281 atcgcgttac cgccatcaga aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt
     2341 aatactgttg aaagcaccag aacagaaaag atcgctgcaa ttgtcttctt caccataacg
     2401 tcttcctttt aggt
//
LOCUS       DADLSB010000304         2385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.307, whole genome shotgun sequence.
ACCESSION   DADLSB010000304 DADLSB010000000
VERSION     DADLSB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.307"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            329..2385
                     /gene="tssH"
                     /locus_tag="JN331_004260"
                     /pseudo
     CDS             329..2385
                     /gene="tssH"
                     /locus_tag="JN331_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459269.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
BASE COUNT          565 a          565 c          662 g          593 t
ORIGIN      
        1 gtatcactgt gtagagtgtt tctttcaaaa gaataagttt tctaaatttt tagataagca
       61 atgctgatca atttacttta tcaatataag ttttcgcgga aatatatatg gcgattttta
      121 atttaatata ttcatggtgt gtatgaaatt ggcttactct attgaatatt accatgaaaa
      181 agatactacc tgctatatta cattgtgttc tgtgatatgc atcattgttt cattctgcct
      241 ttttgataat tctattaatt agaagtttta atcggtacag ttatgtttat gtatcattta
      301 tatgttatta catcatcagg tgtaacagat ggacactcct gtttcgcgca gtgcactata
      361 tggaaagcta accaaatctt tgtttagatc tttggaatct gcgacaacgt tctgtaaatt
      421 acgcgctaat ccttatgtcg aactgacgca ttggttgcat cagttaatac aacaaccaga
      481 gaatgacatt cacaatgtgc ttcgacatta tcaaataaca tctgctgatg ttgagaaggc
      541 gttaattcag caattgaata tattgcctgc cggagcgagt gtaattaacg atttttccca
      601 tcatatcgat ctttctgttg aaaaagcctg gatgttggca agtatccgct ttggcgatca
      661 aaaaattcgt agtggatggt tattacttgc ctggttaacc acgccagaat tgtgtcgggt
      721 gttaaaaaat atttgtgcgc cattggcaga gctaccggta gatgaactat tagatattct
      781 tccgtcttta atcacgtcat ctcctgaatt aacagaaatg ccttatgacg gctcggattt
      841 aaattctgca gtaccggggg aagctagcca ggtctttgcc ggcgaatcaa ctggaagcaa
      901 atccgcgctg gcgaaatact gtcaggacat gactgcgctg gcccgtgaag gcaatatcga
      961 tccggtcacc ggtcgcgaac acgagatccg tactatgacg gatattctgc tgcgccgtcg
     1021 ccagaacaac ccgctactga ccggcgaagc gggagtcggg aaaacggcag tggtggaagg
     1081 attcgcgctg gcgattgccg ggggggaagt accgccttca ctgcgtaacg tgcggttgct
     1141 gtcactggat gttggcgcgc tgctggctgg ggcgtccatg aagggtgaat ttgaatcccg
     1201 cctgaaagcg ttgctggaag aggccgcgca ctcgccgcag ccggtaatcc tttttgttga
     1261 tgaagttcac acgctggtgg tacatccggt acgggggatg ctgccaacct gctgaaaccg
     1321 gcgctggcgc gtggcaccct gcgcaccatc ggcgcaacca cctggagcga gtacaaacgc
     1381 catatcgaaa aggatccggc gctgactcgg cgttttcagg tattgcaggt tgctgagccg
     1441 gaagaggttg cggctgttga gatggtacgc gggttagtca ccacgcttga aaaacaccat
     1501 gacgtgctga tccttgatga ggccgtgcgc gccgccgtac agctttctca ccgttacatt
     1561 ccggcccggc aactacccga taaggccatc agcctgctgg atacggcggc ggcccgtgta
     1621 gcgctgtcgc tgcatacccc gcctgcgaga gtccagtatc tgcgccagca gctcaacgct
     1681 gccgaactgg agctggagtt gttattaaag caggaaaaaa tgggtatccg ctcggagcgg
     1741 ggcgatgtac tggcggcgcg gattatatcg ctgaatgccg atctttctga caccgaagcg
     1801 cggtggcagc aggaactgga actggttcac cgattacagg aacagcgtgc cgcagaaccc
     1861 ggcgagcgtg acgaaatcac gctgcaacag gcagaaacgg cattaagaga ctggcagggc
     1921 gaaaaccccg tcgtgttccc ggaagtcagc gcggcagtgg tggccgcaat tgtttctgac
     1981 tggaccggta ttcccgccgg acgcatggtg aaagatgaag ccagtcagat cctggaactg
     2041 cccgcccgtc tggcgcagca tgttaccggg cagagggacg cgctggcgca gattggcgag
     2101 cgtatccaga ccgcgcgggc gggtttaggt gaccagcgta agcctgtggg cgtctttttg
     2161 ctggccgggc cgtccggtgt tggcaagact gagaccgctc tggcgctggc agaggctatc
     2221 tatggcggtg agcagaacct gatcaccatc aacatgagcg agtttcagga gtcccacacc
     2281 gtttccacgc tgaaaggcgc accgcccggc tacgtgggtt atggcgaagg cggcgtgttg
     2341 acagaagcgg tacgccgcca tccctggagc gtggtattgc tggat
//
LOCUS       DADLSB010000305         2313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.308, whole genome shotgun sequence.
ACCESSION   DADLSB010000305 DADLSB010000000
VERSION     DADLSB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.308"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..517
                     /locus_tag="JN331_004261"
     CDS             <1..517
                     /locus_tag="JN331_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7262191.1"
                     /translation="VDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENKES
                     LFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRSDH
                     SLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYG
                     GSSGTETRPLQ"
     gene            546..>2313
                     /locus_tag="JN331_004262"
     CDS             546..>2313
                     /locus_tag="JN331_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7262192.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVLGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          691 a          544 c          503 g          575 t
ORIGIN      
        1 ggtagataat atagatggta aaagccgtgc cccatttatt ataaccccac cgctatttcg
       61 tcttgaggct ggcgatgact catcactgcg aattattaaa acagctgata acctgcctga
      121 aaataaagag tcgctgttct acattaatgt tcgtgccatt ccagcaaaga aaaaatcaga
      181 tgatgttaat gctaacgagt tgacgctggt atttaaaaca cggatcaaaa tgttttatcg
      241 ccccgcacac ctgaagggac gggtaaacga tgcgtggaaa tcactggaat ttaaacgtag
      301 tgaccattca ctcaatatat ataacccaac tgaatattac gtcgtatttg ccggactggc
      361 agtcgataaa accgatctca caagcaaaat tgaatatatc gcgcccggag aacataaaca
      421 gttaccactt ccagcatctg gcggaaagaa cgtgaaatgg gctgcgatca acgattatgg
      481 cggcagttcc gggacagaaa ctcgtccact gcaataaaaa atataaaaac acaggtcatc
      541 agggaatgcc tcaacgacac caccagggac ataaacgcac accgaaacag ttggcgctca
      601 ttatcaaacg ttgtttgccg atggtgctca ctggcagcgg catgctttgc actaccgcta
      661 acgccgaaga gtattatttc gaccccatta tgctggaaac tacaaaaagt ggtatgcaaa
      721 caaccgatct gtcacgtttt tcaaaaaaat acgcacaact accaggaact tatcaggttg
      781 atatctggct gaataaaaag aaggtttcac agaaaaaaat tacatttacc gccaatgcag
      841 agcaacttct gcagccacag tttacggtag aacaactacg tgagctgggt attaaggtgg
      901 atgaaatccc ggcgctggct gaaaaagatg acgatagcgt gatcaactcg cttgaacaaa
      961 tcattcccgg tacagctgct gaatttgatt tcaatcatca gcgacttaat ttgagcattc
     1021 cccaaattgc actgtaccgt gatgcaagag gttacgtctc cccttctcgt tgggacgatg
     1081 gtataccaac gctgtttacc aactactcgt ttacaggttc tgataaccgt taccgccagg
     1141 gcaatcgtag ccaacgacag tacctgaata tgcaaaatgg tgctaatttt ggcccctggc
     1201 gattacgtaa ctattctacg tggacacgca acgatcaggc gtcaagctgg aacactatca
     1261 gtagttattt acaacgtgat atcaaggcgt tgaagtctca gttgcttctg ggagaaagcg
     1321 ccaccagcgg cagtattttt tccagctaca cctttactgg cgtgcaactc gcttccgacg
     1381 ataatatgtt gccaaacagc cagcgcggat ttgccccaac ggtactcggt atcgcaaaca
     1441 gtagtgcaat cgtgactatc aggcaaaatg gttatgtgat ctatcaaagc aacgtgccag
     1501 cgggtgcctt tgaaattaac gatctctacc cctcttccaa cagcggcgat ttagaagtca
     1561 cgattgaaga aagtgacggt acacaacgtc gctttatcca gccttattct tcattaccca
     1621 tgatgcagcg acctgggcat ctaaaatata gcgcgaccgc tggacgctat cgcgctgatg
     1681 caaacagtga tagcaaggaa cccgaatttg ctgaagccac ggcaatatat ggtttgaata
     1741 atacttttac gctgtatggc ggcctgctcg gttctgaaga ttattatgcg ctggggatcg
     1801 gtatcggcgg cacacttggc gcactgggcg cgttgtcgat ggatatcaac agagctgaca
     1861 cccaattcga taaccagcac tcttttcatg gctatcaatg gcgtacgcag tacatcaaag
     1921 atatcccgga aaccaacacc aatatcgctg ttagctacta tcgctatacc aacgatggct
     1981 attttagttt tgatgaagcc aatacccgca attggggcta taacagtcgc caaaaaagtg
     2041 aaattcaatt caacatcagc cagacaatat ttgatggggt aagtctgtat gcctccggtt
     2101 cacagcaaga ctattggggc aataacgaga aaaacagtaa tatctctgtt ggggtttccg
     2161 gccagcaatg gggaattggt tacagcctga attatcaata cagccgctac actgatgaaa
     2221 ataatgaccg tgcactctct ttgaatctca gtattccgtt agaacgctgg tggatttgcc
     2281 cctatatttc cagacatctg ttatcactta acc
//
LOCUS       DADLSB010000306         2297 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.309, whole genome shotgun sequence.
ACCESSION   DADLSB010000306 DADLSB010000000
VERSION     DADLSB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.309"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1415
                     /locus_tag="JN331_004263"
     CDS             <1..1415
                     /locus_tag="JN331_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7262193.1"
                     /translation="QNLTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDR
                     AAILFKIADRMEQNLELLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGG
                     ISEVDSETVAYHFHEPLGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLS
                     VLLLMEIVGDLLPPGVVNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQ
                     NIIPVTLELGGKSPNIFFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQES
                     IYERFMERAIRRVESIRSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGG
                     RRKLLEGELKDGYYLEPTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQY
                     GLGAGVWSRNGNLAYKMGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLE
                     HYQQTKCLLVSYSDKPLGLF"
     gene            complement(1634..1777)
                     /locus_tag="JN331_004264"
     CDS             complement(1634..1777)
                     /locus_tag="JN331_004264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262194.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            1960..2283
                     /locus_tag="JN331_004265"
                     /pseudo
     CDS             1960..2283
                     /locus_tag="JN331_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
BASE COUNT          562 a          529 c          630 g          576 t
ORIGIN      
        1 accagaacct gacgccggtg accgggcagc tgctgtgcga agtggcgtct tcgggcaaac
       61 gagacatcga tctggcgctg gatgctgcgc acaaagtgaa agataaatgg gcgcacacct
      121 cggtgcagga tcgcgcggcg attctgttta agattgccga tcgaatggaa caaaacctcg
      181 agctgttagc gacagctgaa acctgggata acggcaaacc cattcgcgaa accagtgctg
      241 cggatgtacc gctggcgatt gaccatttcc gctatttcgc ctcgtgtatt cgggcgcagg
      301 aaggtgggat cagtgaagtt gatagcgaaa ccgtggccta tcatttccat gaaccgttag
      361 gcgtggtggg gcagattatc ccgtggaact tcccgctgct gatggcgagc tggaaaatgg
      421 ctcccgcgct ggcggcgggc aactgtgtgg tgctgaaacc cgcacgtctt accccgcttt
      481 ctgtactgct gctaatggaa attgtcggtg atttactgcc gccgggcgtg gtgaacgtgg
      541 tcaatggcgc aggtggggaa attggcgaat atctggcgac ctcgaaacgc atcgccaaag
      601 tggcgtttac cggctcaacg gaagtgggcc aacaaattat gcaatacgca acgcaaaaca
      661 ttattccggt gacgctggag ttgggcggta agtcgccaaa tatcttcttt gctgatgtga
      721 tggatgaaga agatgccttt ttcgataaag cgctggaagg ctttgcactg tttgccttta
      781 accagggcga agtttgcacc tgtccgagtc gtgccttagt gcaggagtca atctacgaac
      841 gctttatgga acgcgccatc cgccgtgtcg aaagcattcg cagcggtaac ccgctcgaca
      901 gcgtgacgca aatgggcgcg caggtttctc acgggcaact ggaaaccatc ctcaactaca
      961 ttgatatcgg taaaaaagag ggcgctgacg tgctcacagg cgggcggcgc aagctgctgg
     1021 aaggtgaact gaaagacggc tactacctcg aaccgacgat tctgtttggt cagaacaata
     1081 tgcgggtgtt ccaggaggag atttttggcc cggtgctggc ggtgaccacc ttcaaaacga
     1141 tggaagaagc gctggagctg gcgaacgata cgcaatatgg cctgggcgcg ggcgtctgga
     1201 gccgcaacgg taatctggcc tataagatgg ggcgcggcat acaggctggg cgcgtgtgga
     1261 ccaactgtta tcacgcttac ccggcacatg ctgcgtttgg tggctacaaa caatcaggta
     1321 tcggtcgcga aacccacaag atgatgctgg agcattacca gcaaaccaag tgcctgctgg
     1381 tgagctactc ggataaaccg ttggggctgt tctgatataa gaagctggtc gcattgggta
     1441 ttcattgcct gatgcgacgc ttacgcgtct tatcatgcct acgggaacct gaccgtaggc
     1501 cggataaggc gtacacgccg catccggcat aaacaaagcg cacgttgtta caatttacgt
     1561 tttaaaccta cgtgcgcaat aacccagact acgccaacag aagtgcgttt ctggggcgtg
     1621 ttttgtcagg agtttaatct cgcgtttcga tggtgagcgg gcgaaccagg ccgtaaatca
     1681 ccattggtga tccgaggcca atcagcaata aacggtagat aattgaaggt ttaatacgct
     1741 taatctgtac tgtggatgtt ttactggagt tattcattag tttggccaat agttccacta
     1801 tttcaatatg ataaacctcg tcctgttgaa aaatcagaca gcacatttca gaaagtatat
     1861 aaaaaacaat atgtcgttat ataaataagg aaattgcact gcatcatggc atttcataat
     1921 atgcgttaat tgttcttttt tatattcttt gagggaaata tgttcctgga ctattttgca
     1981 ctgggagtgc ttatttttgt atttctggtg atattctatg ggattattat tttacatgat
     2041 attccctacc tgattgctaa aaaacgtaat catcctcatg ccgacgccat tcatgttgct
     2101 ggttgggtga gtctttttac gctgcatgtt atctggccat ttttgtggat ttgggccacg
     2161 ctttaccgcc cggagcgggg atggggaatg taaaaccatg actcctccgt tgtgcaacta
     2221 caacagcgta ttgccgggct ggagaaacag ctcgccgaca tcaaatcctc ttctgccgag
     2281 taatacttat ggattta
//
LOCUS       DADLSB010000307         2258 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.310, whole genome shotgun sequence.
ACCESSION   DADLSB010000307 DADLSB010000000
VERSION     DADLSB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.310"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN331_004266"
     CDS             complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN331_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY7262195.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(1803..>2258)
                     /locus_tag="JN331_004267"
     CDS             complement(1803..>2258)
                     /locus_tag="JN331_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAY7262196.1"
                     /translation="SAHWPLQGSNDKGNNHSCVVKVDDGTNSILLTGDIEAPAEQKML
                     SRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKVALASASRYNAWRLPSNKVKHRYQ
                     QKGYQWLDTPHQGQVTVNFSAQGWRISSLREQILPRWYHQWFGVPVDNG"
BASE COUNT          585 a          590 c          533 g          550 t
ORIGIN      
        1 agaccagatt ttttcgatca ttggccaaac tgcattttgt gaagttgcgc gtaaacgccg
       61 cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa tgacaccatc ctcgacgacc
      121 acgatttcgt ctgccttttc aatggtagac aagcggtggg caatcaccag agaggtacgg
      181 tttttctgca actcatccag tgccgcctga atcgcacgtt cggattcggt atccagagcc
      241 gaggtagctt catccagaat cagtatcggg ctatcacgca gcaaggctcg agcgatagca
      301 atacgctgac gctgaccgcc agagagcagc acgccgtttt caccaatcac tgtatcgaga
      361 ccgttatcca tcttattgat gaagtccatg gcgtaggcca tacgcgccgc ttcttcaatt
      421 tgctcacggc tgtactgttc agtccgtgcg taagcaatgt tgttagcaac cgtatcgtta
      481 aacagatgga cattctgcga caccagagca acctggttac gtaacgacgc cagggtatac
      541 tcgcgcagat cgtgaccatc catcaggatt tcgccttcat caatatcgta aaaacgcgtg
      601 atcaggctgg cgatggttga tttacctgaa ccagagcgtc caaccagagc aaccgtcttc
      661 cctgccggaa ttttcaggtt gatgttacgc aatgcgggta cgtcacgtcc cggataagta
      721 aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct cgatcacgcg cttaccttcg
      781 tctttctcct gctcactgtc cagaatggta aacagcgtct gacaagccgc cataccgcgc
      841 tggaactggg cgttaacgtt ggtcagcgat ttcagcggac gcatcagtgc aatcattgaa
      901 gagaaaacaa cggtaatcgt accggcagtc aggttatcca taacacttgg gaagctcgcc
      961 gcatacagaa caaacgccag cgccaaagag gcgatcagct gaatgatcgg atcagagatg
     1021 gaagaggctg aaaccatttt catcccctga agacgcattc ggttactgac tttatcgaag
     1081 cgtttcgttt ccacttcctg accaccgaaa atcaatactt ctttgtggcc cttcagcatt
     1141 tgttctgcac tggtggtcac ttgccccatg gtgttctgca tgtttttact gatgttgcga
     1201 aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg gtgccagcac aatcaaaatg
     1261 atcgacagtt gccaactgta atagaacatc atgatgaaca ggccgatgat cgacgcacct
     1321 tcacgcacaa cagtaatcag tgcgccggaa gaagaagaag caacctgttc ggaatcgtag
     1381 gtgatacgtg acaacagcgt acccgttgac tgtttgtcaa agaatgaaac tggcattccc
     1441 atcatgtgac caaacaggcg gcgacgcatg gtcattacca cctttcctga tacccaggag
     1501 atacagtagc tggagacata gctggtgata ccacgtaaaa tcatcagtcc gatcaccacc
     1561 agcggcatcc acaccagcac ggagcgatct gttttaccaa agccatcatc aagaagtggc
     1621 ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga ggattaacgc tacgcccgcc
     1681 acgatcagac ccgctttgaa aggcgcaatg gttggccaca gtcggcggaa tgtctgccac
     1741 gtagagagat ctttgtcgtt atgcattcaa aaaaccagca tttgttgaaa tagccgcata
     1801 ttctacccgt tatccactgg cacgccaaac cactgatgat accaacgagg taaaatttgc
     1861 tctctgaggc tgctaatccg ccagccttgt gctgaaaaat tgaccgtcac ttgaccctga
     1921 tgtggagtat caagccattg atatcctttc tgttgatagc gatgcttaac tttgttagag
     1981 ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc
     2041 tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagcaatgtt
     2101 gcctgcactt gctgccagta acggcttagc atcttttgtt cagctggggc ttcaatatca
     2161 ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa ccacacagga atggttattt
     2221 cccttatcgt tgctaccttg taaaggccag tgcgcgct
//
LOCUS       DADLSB010000308         2248 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.312, whole genome shotgun sequence.
ACCESSION   DADLSB010000308 DADLSB010000000
VERSION     DADLSB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.312"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..881
                     /locus_tag="JN331_004268"
     CDS             <1..881
                     /locus_tag="JN331_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7262197.1"
                     /translation="GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWL
                     TLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLT
                     VMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDID
                     HFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKAL
                     AERIRENVERLTGDNPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETG
                     RNKVVVRDVVNFCESP"
     gene            979..1977
                     /locus_tag="JN331_004269"
                     /pseudo
     CDS             979..1977
                     /locus_tag="JN331_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2012..>2248)
                     /locus_tag="JN331_004270"
     CDS             complement(2012..>2248)
                     /locus_tag="JN331_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7262198.1"
                     /translation="RHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMR
                     ATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
BASE COUNT          662 a          420 c          481 g          685 t
ORIGIN      
        1 gggggccgat agttgctcac atactttcta gtcattatga gagctacaat ttacacattg
       61 ccgagttaac caatgaaaat ggtcaggtgg tttggaaagc ctcatatgtt accataatga
      121 ttttcatgtg gttaaccttg ctttcggtta atctatactt taatggttta cgctatgata
      181 tctggaatgg agtgacggtg attgcttttt gtgccgtact gtacaatatc tctctgttat
      241 ttatgagcag gtatagcgtg tccacttggt atattagtcg cactattgaa gtagtcagta
      301 agttaaccgt tatggtaata tttatgtgcc atatattcag tgcgctacga gtaacaaaga
      361 acattgcaca tcgcgatccc ttaaccaata tatttaacag aaattatttt tttaatgaac
      421 tgacagttca atcagcatca gccaaaaaaa cgccttattg tgtcatgatt atggatatcg
      481 accacttcaa aaaagtcaac gacacctggg ggcatccggt tggcgatcag gtgataaaaa
      541 cagtggtgaa tatcattggg aaaagcatac gaccagatga ccttttagcg cgcgtcggcg
      601 gcgaagagtt tggcgtcttg ctgacggaca tcgatactga acgtgcgaaa gctttagcgg
      661 aaaggattcg ggaaaatgtt gagcgtttaa ctggcgataa tcctgaatac gctataccac
      721 aaaaagtgac gattagtatt ggcgctgttg tgactcagga aaatgcgtta aacccgaatg
      781 agatctatcg actggctgat aacgcactat acgaggcgaa agagaccggg cgtaataagg
      841 tggttgtgag ggatgtggtg aatttttgtg agtcaccata aagcggcatt ttagcgggtg
      901 attttggtaa gcgctaaata gctattcgca gacttgcgta acaattttca gaaatatcat
      961 agtactatat gatcttaaat gcttatcatt gatcaatctg ctatcacccg cttttactgg
     1021 caattgtact ataatttctt aggttgcatc accctgatgc gtggggaaac atgtatggta
     1081 ttcaacaaaa aaatgtttgt actcattata atactaggca ttctgggggt gttactatcg
     1141 tttgctatgt caatatttca aatgaataga gatacgacaa ttaccgccgg tattctgtta
     1201 aaacagcttg ataatgttac gcaaattgtc cagcatacga caaagcttac ctctattctg
     1261 gtgatgaagc cctgcaaaga cattcttgaa cagttgattg ctaacggagc attgactccc
     1321 tatgttcgaa caacgggtct tatagaaaat aatttccaaa tatgttcttc agttagcggt
     1381 ttcaaaaaaa tgaatgttaa cgatgtttac ggtacgagct ttcacaataa aaataaagaa
     1441 tcacgtatag tatcaattag cggtacaagt tttgtgccag gtaaaacagc aatagtgttc
     1501 cttatgccaa tagggaatga tatgacagct tttagtattg tagagtcacg atatatctat
     1561 gatctaatgg atgttcttga tgatgagaat gatgattcat tttctcttcg ttttactgaa
     1621 gggcctgcaa ttattagcgg tgtaaataat aatgacaggt tatatatgtt aaaaagagat
     1681 tttaattctg cgatcagtca ggctcgtctt acagttacta cgccgatgat atcgttatat
     1741 ccctatatca ttagcaatgt gctttatatt ttgccgttat ctattattct ctcttttatt
     1801 ctctatttct tatggcagca ctggatgtca cggaaaatgt cgctggcaga agaaattaaa
     1861 aaaggaatgt catccggaga attttctgtg cattatcaac cagtgtgcga cacgacaacc
     1921 aaagcgtgtc tgggggttga agcgttaatg cgctggcaac gcgaagatgg taaaaatata
     1981 ttgatcctac ccacgtaata tggacacagg cctaagcgag gttctggttt tcaaattgtt
     2041 ccggactgag gccgccacac caactgtgcc gccgccaccg attgtaatca cattcgatat
     2101 aattaaacac cgttgcccgc attatttccc ggctgataaa gtgttctcca tggatacatt
     2161 ccactttcag cgaatgaaag aagctttcca cgcaggcatt atcgtagcag caaccttttg
     2221 cgctcatact tccacgcaga ttatgccg
//
LOCUS       DADLSB010000309         2245 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.313, whole genome shotgun sequence.
ACCESSION   DADLSB010000309 DADLSB010000000
VERSION     DADLSB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.313"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            11..565
                     /locus_tag="JN331_004271"
     CDS             11..565
                     /locus_tag="JN331_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7262199.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            667..1158
                     /locus_tag="JN331_004272"
     CDS             667..1158
                     /locus_tag="JN331_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY7262200.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1222..1997)
                     /locus_tag="JN331_004273"
                     /pseudo
     CDS             complement(1222..1997)
                     /locus_tag="JN331_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(2024..>2245)
                     /locus_tag="JN331_004274"
     CDS             complement(2024..>2245)
                     /locus_tag="JN331_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HAY7262201.1"
                     /translation="SALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDG
                     QLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
BASE COUNT          522 a          531 c          591 g          601 t
ORIGIN      
        1 aaatatatta atgaacgagt tttctatcct ctgtcgtgtg ctgggttcgc tctattaccg
       61 ccaaccgcaa gatcctttac tggtgccgct gtttactctg attcgtgagg ggaaactggc
      121 tgcgaactgg ccactggagc aggatgagtt actgacgcgt ttacagaaaa gctgtgatat
      181 gacgcaagtc tctgccgatt acaatgcgtt gtttatcggc gatgaatgtg ccgtgccgcc
      241 atatcgtagc gcatgggttg aggacgcaac ggaagcggaa gtgcgcgctt ttctttccga
      301 acgagggatg ccactggcgg atacgccagc cgatcacatc ggcacattgc tgcttgcggc
      361 ttcctggctg gaagatcagt caacggaaga tgagagcgaa gcactggaaa cactgttcag
      421 tgagtatctg ttaccctggt gtggtgcgtt cctcggcaaa gtggaggccc atgcaaccac
      481 gcctttctgg cgcaccatgg caccgctaac ccgtgatgcc attagtgcaa tgtgggacga
      541 gctggaagaa gattctgaag agtaaatgtg atcatcgtca ctcataactg caacagataa
      601 tttgttgttg cataaaatgt gtgctcgatc tcattcatgg ccgcgttttc tgctatcatg
      661 cgcggcatga acatacttct ctctattgca atcacaacag gcattctctc cggtatctgg
      721 ggatgggtgg ctgtttccct gggcttacta agctgggcgg gcttcctggg ctgtacggcc
      781 tactttgctt gcccgcaagg tgggctgaaa gggctggcga tctccgctgc aaccctgctt
      841 agtggcgtgg tgtgggcgat ggtcattatt tacggtagtg cactggcacc acatctggaa
      901 attctcggtt atgtcataac cggtattgtc gcgtttctga tgtgtattca ggccaaacag
      961 ctgctgcttt catttgttcc ggggacgttt ataggcgcat gcgcgacgtt tgccgggcag
     1021 ggtgactgga aactggtgtt accttcgctg gcgcttgggc tggtatttgg ttacgcaatg
     1081 aaaaacagcg gcctgtggct ggcggcgcgt agtgcaaaga cggctcaccg tgagcaggaa
     1141 atcaaaaata aagcgtgagg ggcactcacg ctttcgctta aacagtaaat gccggattat
     1201 taattccggc atttttatct gtcaggattc cggtggaacc gacatatggc ggtatttcac
     1261 cagaatgtca ttctgccgtt ctgctttatt ttgcaaatcc cacagaccac ggtcgatacc
     1321 atcattaatc aggaaaatga cccctgtttc gatagccgac atcagacaca gcataacggg
     1381 ttcgtttgag gtgtaaccca cttccccttc cagcaagcgc tggtagtcaa taaagcggaa
     1441 aaccccggcc tgaacttcat aggaaagtat cgtcttactg gtgttcaccg aggagaggat
     1501 ctcaccggta ctcacattga cgacacgcag gttcacggca atctgatcga gctggtattg
     1561 cgtgtctgca ccgatcccaa aatatcttgc cccaacaccg cccgatttaa cgttgctttc
     1621 ataaccgata atcgaacctt caaccataat atttgccgcc gttaaagatt gcagcgggat
     1681 tcggttatta atggcaaccg tgccgttttc ttgtgccgca cgaataatct tgcgctcgtt
     1741 aagcaggttt tgtaagccct ggcgctttgc ggaacagcag tggagaagtt acttgccggg
     1801 tagggtttaa attgcctggt ttcgtcctga atgttgtata ccgaaacaaa gatcttaccc
     1861 gtcggcgctg gcagatgggt caaatctttg tagctctgag cacgaggcat taatgtcggt
     1921 ctggcggctt ctttaggcgg ggcggttaag catccgctca gtaacatgac ggcaaccaaa
     1981 agaaataagc gctgcatgat tattttcctt atgaagctgg ggcttaaaaa tcggttgagt
     2041 tattttgtaa acccgaaacc tggatggtcg aggtttgtcc ggttttacga tctgtcacgt
     2101 tcaactgcaa ttgaccatcg cggttagcaa tatcaacaat ataatcgttg gtcaccatgc
     2161 ggcccggttt accggtatta atattcgaca gtagcccacc taaaatttgt gactggatgg
     2221 cctgagtaaa gttatctaac gctga
//
LOCUS       DADLSB010000310         2227 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.314, whole genome shotgun sequence.
ACCESSION   DADLSB010000310 DADLSB010000000
VERSION     DADLSB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.314"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1026
                     /locus_tag="JN331_004275"
     CDS             <1..1026
                     /locus_tag="JN331_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HAY7262202.1"
                     /translation="LLPFAHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQL
                     CDYDLLGFQTENDRLAFLDCLSNLTRVTTRRAKSHTAWGKAFRTEVYPIGIEPKEIAK
                     QAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRY
                     TQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRY
                     SDVGLVTPLRDGMNLVAKEYVAAQGPANPGVLVLSQFAGAANELTSALIVNPYDRDEV
                     AAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAESQQRDKV
                     ATFPKLA"
     gene            complement(1033..1461)
                     /gene="uspC"
                     /locus_tag="JN331_004276"
     CDS             complement(1033..1461)
                     /gene="uspC"
                     /locus_tag="JN331_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HAY7262203.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT          624 a          479 c          486 g          638 t
ORIGIN      
        1 ctgttgccct ttgcgcatga attacgcaaa cgtggggtga ataatcgcat tggttttttc
       61 ttgcatattc ctttcccgac accggaaatt ttcaacgcgc tgccgacata tgacaccttg
      121 cttgaacagc tttgtgatta tgatttgctg ggtttccaga cagaaaacga tcgtctggcg
      181 ttcctggatt gcctttctaa cctgacccgc gtcacgacac gtcgcgcaaa aagccataca
      241 gcctggggaa aagcatttcg aacagaagtc tacccgatcg gcattgaacc gaaagaaata
      301 gccaaacagg ctgccgggcc actgccgcca aaactggcgc aacttaaagc ggaattgaaa
      361 aacgtacaaa atatcttttc tgtcgaacgg ctggattatt ccaaaggttt gccggagcgt
      421 tttctcgcct atgaggcatt gctggaaaaa tatccgcagc atcatggtaa aattcgttat
      481 acccagattg caccaacgtc gcgtggtgat gtgcaagcct accaggatat tcgtcatcag
      541 ctcgaaaatg aagctggacg aattaatggt aaatacgggc aattaggctg gacgccgctt
      601 tattatttga atcagcattt tgaccgtaaa ttactgatga aaatattccg ctactctgac
      661 gtgggcttag tgacgccact gcgtgacggg atgaacctgg tagcaaaaga gtatgttgct
      721 gctcagggcc cagccaaccc gggcgttctt gttctttcgc aatttgcggg agcggcaaac
      781 gagttaacgt cggcgttaat tgttaacccc tacgatcgtg acgaagttgc agctgcgctg
      841 gatcgtgcat tgactatgtc gctggcggaa cgtatttccc gtcatgcaga aatgctggac
      901 gttatcgtga aaaacgatat taaccactgg caggagtgct tcattagcga cctaaagcag
      961 atagttccgc gaagcgcgga aagccagcag cgcgataaag ttgctacctt tccaaagctt
     1021 gcgtaggagc ggttaatctc ccgtaagtgg aactaacagc acatcgacct cacttgaggc
     1081 aatcactctt ttcgccgagc aggatgctcg cgaaaagaaa ctgtgattgt gattaccgca
     1141 aatcaccaaa ttgaaatgat gtttgtgaca tacttccaga atatgttcgc ttaactcacc
     1201 gtaggcaata aacgtcttgt caacgggata ccccgcatcc tgaattaact tatcaagaaa
     1261 gctttgcgtt tcttcttgca tcacgctgcg taaatcttca agcatcggcg cggctaattg
     1321 attgtacatt tccgggtcgg aagcgagagt aattaaactg atgtgtccct ttactggcct
     1381 ggcgatagat accgcttttg ccagcagttg ctgactttcc ggtgtaaccg caacagcgac
     1441 aagaatattg ctatagctca taacctgttc cttattctgt gaacttcagg tgacattaaa
     1501 gctttactcc taatggagaa acgacgcaat cccaactcag tcaaacaggt aaatattagt
     1561 ctgattatta gtaaagacaa ttaatcaatc actctcggtg atcatatgat ttcaactgta
     1621 attataagaa aaaaatgtac tgattccccg cattaaacaa gtggctaata tatgaaaaac
     1681 attttattaa cattaagttg attgttgcct ttctttgtat ttaattagtt tgttgtgcgg
     1741 taagtgtctg tttaaaaata gcttatctgg tattgcatga aagtgattat ttatagcaga
     1801 tgattattta cggtgagtta ttttaaccgt gcgcaacatc ccatttcgat tattcctgtt
     1861 tcatttttgc ttgctagcgt agcgaaaaac tttttaacag attgaaatac acccaaaaca
     1921 aaagtatgac ttatacattt atgttaagta attgagtgtt ttgtgtgatc tgcatcacac
     1981 attattgaaa atcgcagccc ccctccgttg tatgtgcgtg tagtgacgag tacagttgcg
     2041 tcgatttagg aaaaatctta gataagtgta aagaccccct tctatttgta aggacttatt
     2101 aaaccaaaaa ggtggctctg cttattgcag cttatcgcaa ctattctaat gctaattatt
     2161 ttttaccgga gcttcccggc gacatcacgg ggtgcggtga gaccgcataa aaataaagtt
     2221 ggttatt
//
LOCUS       DADLSB010000311         2222 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.315, whole genome shotgun sequence.
ACCESSION   DADLSB010000311 DADLSB010000000
VERSION     DADLSB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.315"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            125..1098
                     /locus_tag="JN331_004277"
                     /pseudo
     CDS             125..1098
                     /locus_tag="JN331_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1095..2186)
                     /locus_tag="JN331_004278"
     CDS             complement(1095..2186)
                     /locus_tag="JN331_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7262204.1"
                     /translation="MFKIFKIPVNKWTISTAALGGIFIVSGLILLMNYNHPYTFKAQK
                     AVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQARVDRLMADIVTAEHKQ
                     RALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFSERDIDVARQNYLAQE
                     ASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLEQTIVRAPSDGYVTQV
                     LIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGDDAEVVFNALPGKVFS
                     GKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHTDLSALPDGIYAQVAV
                     YSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
BASE COUNT          584 a          558 c          515 g          565 t
ORIGIN      
        1 tgtccggtat tccattttaa aataagataa aaaagagtcg gcataataat gcttacaatt
       61 tctccggtgc aagattcata tttatatagt aatatataaa attatatata attgggctgt
      121 tgcgatgaca aaattacaac ttaagtaccg ggagttaaaa attatctcgg taatcgctgc
      181 cagtgaaaat atcagccatg ccggactgta cttggcatcg cacaggccaa cgtcagcaaa
      241 tatcttgctg attttgaatc aaaagtgggt ttaaaagtct ttgaccgaac cacccggcag
      301 ctgatgctca caccttttgg caccgcgctg ttaccgtaca tcaacgacat gctggacaga
      361 aacgagcaac ttaataattt tattgcagat tataagcatg agaaacgtgg tcgggtcacc
      421 atctacgccc caaccggcat aattacctat ttatccaaac atgtaatcga taaaattaaa
      481 gatatcggtg atatcacctt atcgctaaaa acctgcaatc ttgagcgtaa tgccttttat
      541 gaaggcgtcg aatttcctga tgattgtgat gttttaatca gctacgctcc accaaaagat
      601 gaatcgctgg ttgccagttt tattacacaa tatgccgtaa ccgcttatgc cagccagcgc
      661 tatcttgaga aacatcctat tagccgtcct gacgaactcg aacatcactc ctgtattttg
      721 atcgactcga tgatgatcga cgacgcgaat atctggcgct tcaacgtcgc cgggagtaaa
      781 gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg ataacacgca atcggcgctg
      841 gagctggcac gaaatcacct ggggattgtg tttgcgccag acaaaagtgt gcagagcgat
      901 ctgcaagacg gcacgctggt gccctgcttt cagcacccgt atgaatggtg gctggatctg
      961 gtggctatct tccgtaaacg ggaatacctg ccctggcggg tccagtatgt gctggatgaa
     1021 atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc aactgcggcc tgaacaagcg
     1081 gcggaaagcg aagattaatg atcgagataa aggtaatgca cccagctggt catgcgtaat
     1141 agcactttgc gcatcaccga gacatggctg aaatgatcag agtagaccgc cacctgggcg
     1201 taaataccgt ctggtaaagc gctcaaatca gtgtgctcat ccagttcaat ggtcgcgata
     1261 acgccatctg aacccggcgc tgtgtttaac gtctgtaagg tgccggtcga ctgataagct
     1321 ccgccgggaa cagctggact aatggctgcc agcttgccgc tgaatacctt acctggcaga
     1381 gcattaaaca ccacttccgc atcgtcgcca ggggccagtc gcagcaagga gttctgacgg
     1441 aactgcgcca caatttgtcg tttctgatcg ggtataaaca ccatcaccgg acgtagcggc
     1501 agcgacgcgg cataggtacc cggacgaatc agcacctggg tcacataacc atcgctcggc
     1561 gcacgcacta tcgtctgctc aaggttatat tttgcttccg cgagctgcgc tttcaggctg
     1621 gcgatttgag aatgttcacc caacaccagg ctatccagct ggctctggat ctgtttttgt
     1681 tccgccgccg atgactttac ggaggcttcc tgcgccagat aattttgccg cgccacatca
     1741 atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc cgcgtgcgta acgctgatac
     1801 tctttagcga atttatcccg cgtggccttt gcctgctgag tattcgccgc catctcatct
     1861 aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa cgatatccgc catcagccga
     1921 tccacccgcg cctgataacg cgtcgggtcc agtcgaaata gcacctcacc ttttttaatc
     1981 agcgtatttt tcttatccgt cacttcgatc accacgcctg tcacctgtgg gacaacagga
     2041 atagaaataa ccgctttttg cgctttgaag gtatacggat ggttatagtt cattaacaga
     2101 attaaaccac tgacaataaa tatgccaccc agggccgctg tggaaatggt ccatttattt
     2161 acaggaattt tgaagatctt aaacattgcc catgcaaaag ccacataggt caaaataatc
     2221 ag
//
LOCUS       DADLSB010000312         2168 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.316, whole genome shotgun sequence.
ACCESSION   DADLSB010000312 DADLSB010000000
VERSION     DADLSB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.316"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            207..1922
                     /locus_tag="JN331_004279"
     CDS             207..1922
                     /locus_tag="JN331_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAY7262205.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            1919..>2168
                     /locus_tag="JN331_004280"
     CDS             1919..>2168
                     /locus_tag="JN331_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7262206.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNIST"
BASE COUNT          461 a          558 c          563 g          586 t
ORIGIN      
        1 tttcgttttt tacatctgaa ctttgcaatt ttccattcat tttaatatcc ctccggcgaa
       61 ttatgctcac aacctgaact taacagaaaa caatccgttc agggatacga cgtttgtatt
      121 ttaagaatct gactgcctga cccgacgcat tttacctctc cctatattca tgcgtccggg
      181 acataaacat aaataagggc tatgagatga attcgttaca aatcttgagt tttgtcggtt
      241 ttacgctgct ggtggcggtg atcacctggt ggaaggttcg caaaacagat accggatcgc
      301 aacaaggcta ttttcttgcc ggacgttcac taaaagcgcc ggttattgcc gcttcgttaa
      361 tgttaaccaa cctttccacg gaacaactgg ttggtctttc cgggcaggcc tacaaaagcg
      421 gcatgtcggt gatgggctgg gaagtgactt cagcggtgac gctgatcttc ctcgcgctaa
      481 tctttttacc gcgctatctg aagcgcggca ttgccaccat ccccgatttt ctggaggaac
      541 gttatgataa aacgacgcgt attatcatcg acttctgctt cctcattgcc accggcgtct
      601 gctttctgcc gattgttctc tactccggcg cgttggcgct caacagcctg tttcacgtcg
      661 gggaatcgct acagatttct cacggtgcgg ctatctggct actggtaatt ttgcttggtc
      721 tggcgggaat tttgtatgcg gtgatcggcg gactgcgcgc aatggcagtg gcggactcca
      781 tcaacggtat tgggctggtt atcggcgggt tgatggtgcc ggtatttggc ctgatcgcga
      841 tgggcaaggg cagctttatg cagggcattg agcaaattac caccgttcac gccgagaaat
      901 taaactcagt cggtggcccg accgatccct tgccgattgg cgcggcattt accggtttaa
      961 ttctggtgaa caccttttac tggtgtacaa atcagggcat cgtgcaacgc acgctggcgt
     1021 caaaaagcct ggcggaaggg caaaaggggg cgctgttaac ggcggtgctg aaaatgctcg
     1081 acccgctggt actggtgctg ccagggttga ttgcgtttca tctgtatcag gatctaccga
     1141 aagccgacat ggcctacccg acgctggtca ataacgttct gccagtgcca ctggtgggtt
     1201 tcttcggcgc ggtgttattt ggtgcggtga tcagtacctt caacggcttt ctgaatagcg
     1261 ccagtacatt attcagtatg ggtatttacc gtcgcatcat taaccagaat gccgagccgc
     1321 agcagctggt caccgtcggg cgcaaatttg gtttctttat cgctatcgtt tcggttctgg
     1381 tagcgccgtg gatcgccaac gcgccgcagg ggctgtatag ctggatgaaa cagctcaacg
     1441 gcatttacaa cgtgccgctg gttaccatca tcattatggg ctttttcttc ccgcgcatcc
     1501 cggcgctggc ggcaaaagtg gcgatgggga ttggcataat cagctacatc accatcaact
     1561 atctggtgaa gttcgacttc catttcctct atgtgctggc ctgtacgttc tgcatcaacg
     1621 tggtcgtgat gctggtgatc ggttttatca aaccgcgcgc cacgccgttc accttcaaag
     1681 atgcgtttgc ggtggacatg aaaccgtgga aaaacgtcaa gatcgcgtca attggtatcc
     1741 tgttcgcgat gattggcgtc tatgtcgggc tggctgaatt cggcggctac ggtacgcgct
     1801 ggttagcgat gatcagttat ttcatcgctg ccgtagtgat tgtctacctg atttttgaca
     1861 gctggcggca tcgtcacgac ccagccgtaa cctttactcc cgacgcgaag gatagcctat
     1921 gaaacgcccc aattttctgt tcatcatgac cgatacccag gccaccaata tggtcggttg
     1981 ctatagcggt aaaccgctga atacgcaaaa tattgatagt ctggcggcgg aaggtattcg
     2041 ctttaattcc gcctacactt gttcaccggt ttgtacgcct gcacgcgccg gactattcac
     2101 cggtatctac gctaaccagt ccggcccgtg gaccaacaac gtcgcgccag gcaaaaacat
     2161 ctccacta
//
LOCUS       DADLSB010000313         2106 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.317, whole genome shotgun sequence.
ACCESSION   DADLSB010000313 DADLSB010000000
VERSION     DADLSB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.317"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..814
                     /locus_tag="JN331_004281"
                     /pseudo
     CDS             1..814
                     /locus_tag="JN331_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1253..2090
                     /locus_tag="JN331_004282"
                     /pseudo
     CDS             1253..2090
                     /locus_tag="JN331_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          523 a          511 c          632 g          440 t
ORIGIN      
        1 ccagtacacc tcagttctgg gccttgcccg tagcagttat ttttatcacc gggctgcact
       61 gaaagccggt gataaatacg ccacgatacg taagatgctg acagatatct ttaacagtaa
      121 ttaccagtgt tatggctatc gtcacctgca tgcgatgctc aggcatgagg gggggcggct
      181 atcagaaaag gttgtacgca gacttatggt ggaggaacag cttgttgtca gtcgtaaccg
      241 tcgtcgtcgc tacagctcat attgcggaga aatcggaccg gctccggata accttatcgc
      301 cagagatttt aaggcggagc aacctaatca gaaatagctg acagatatca cggagttcca
      361 gttccctgca ggtaaagtct ggctgtcacc agtggtggac tgcttcgatg gaaaagttgt
      421 gagctggtct ctcagtacac gccctgatgc tgaactggtc aacactatgc tggatagcgc
      481 tgtcgaaacg ttaaatgctg gcaaacgacc ggtgatacac agtgacagag gtgggcatta
      541 tcgctggcca ggctggctgg aaagagtgaa tgcagcaggt cttattcgct caatgtcccg
      601 taaaggatgt tcacctgata atgctgcatg cgaaggcttt ttcggcagac tgaaaacgga
      661 aatgtattat gggcgtaaat ggtcgggcat cacgccagaa aagttcatgc agcaagtgga
      721 tgcttacatc agatggtata acgagcggcg tataaaatta tcgctgggtg ccgtcagccc
      781 caaaatgtac cgccaacaat gcgggctgga atgataaagc agtccaggaa atcgtccgca
      841 tccccccttc accatttgga ttgtccgctc cacccaacat gttgtttcct taaggttcct
      901 acaccacaaa ggaaactctc atgctgcgca gtggatggct gacgttgccg acaggcggga
      961 acttcgccag ttcaggcaga caccggaaca gcgcttcacg caggaacagg agcatctgca
     1021 gccgctgctg ggtacggact tcgatatccg tcatgtctcc tgggatggct atatcgaggt
     1081 tggcgggaac cgttacagtg tgccggaaag cctgtgtggt cagctggtat caatacggat
     1141 ctcgctggat gatgaactgc ggatctacag taatgagcaa caggtagcat cgcaccgtct
     1201 ctgttcggct gcatacggct ggcagaccgt gccggagcat cacgctccac tctggcagca
     1261 ggcttcggag cgcatggccg aacggctggg ggagatacag aaacgggtaa tcactgtctg
     1321 tgaccgggaa gcggatatct ggcattacct gtattataaa gtcagccacg gacagcgtgg
     1381 tgcgtgctgc acagaatcgc cggctggaag aggcacccgg caagctcttc gaactgccgg
     1441 aagtcctggc aaccgcagga agccacacgc tgaatgtgat gcaaaaaggc gggcgggcag
     1501 cccgtcaggc ccggatgttc atcagttaca gcgaagtcag cataaaaaat cccgacaaca
     1561 gcggccaggc gctcccgctc acgtatgtct gttgccggga gcaggcagag gacggtgcct
     1621 gctggcatct gctgacgtca ggaaaagcgg cgagtgccgc cgatgcacga cgtattgtca
     1681 gccattacga gcgacgctgg ctgacagagg aataccacaa ggcgtggaaa agtggtggta
     1741 catggaatcg ctgcgaatgc agacccggga taaccttgag cgcatggtgg ttatccaggc
     1801 gtttatagcg gtacgggtgc tgggcctgcg gcagggaggt gtcagtgaag aaacacaaaa
     1861 tgacagctgt gagaaaatac tgacgccgac agagtggaaa ctactgtggg ttaagctgga
     1921 aggaaagccg cttccggtgc aggcaccgac cctgaaatgg gcctggggcg atggcatgac
     1981 agcaaacgca caggtcgtcc cggttggggc gtcatgtggg atggctggtc agacttcagg
     2041 atatggttga gggttacctg gtgatgaagt ctcttgatca ggagctctga tcaagagaca
     2101 gcagct
//
LOCUS       DADLSB010000314         2091 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.318, whole genome shotgun sequence.
ACCESSION   DADLSB010000314 DADLSB010000000
VERSION     DADLSB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.318"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>2091
                     /gene="ehaA"
                     /locus_tag="JN331_004283"
     CDS             <1..>2091
                     /gene="ehaA"
                     /locus_tag="JN331_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HAY7262207.1"
                     /translation="VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNN
                     GGEVNAREHISLGYYDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAG
                     IIDAEKIEFVWASTSDKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNS
                     AFSGKVKIEQNGALGITQNIGTAEINNCGKLHLKADDSMTFANKISGNGTISIDSGTV
                     ELTGNNYAFSGYIDVASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRG
                     IVEINMGNHEFSFDEFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVG
                     KGAHSISTLGFSGGTVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVS
                     RDIDSALSLTEVDDGNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNG
                     QKAAVGTYDYRLTSGVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKI
                     TGSGDLAFSSQKGQTVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETEL
                     DMNGHSQTVGTRNGSADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDN
                     SNLTANVNIANSANVLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTN
                     NGTLMTGMSGQQAG"
BASE COUNT          565 a          443 c          597 g          486 t
ORIGIN      
        1 tgttaagggc accagtcgtc tgacttatac gcaggggaat gtaagcggcg aaggtagtca
       61 ggtaaatgcc gggaccttct ttatgggcgt ttacggcgga tatggcggta atcagttcct
      121 gtcggttaat aacggtggtg aagttaatgc tcgtgaacat attagcctgg gttattacga
      181 ccaacgttct gatacaacat tagtcgtatc agatggaggt aaaatatccg caccgaaaat
      241 tagtttaagc accaactctg agttagcgtt aggtgcacag gaaggaagtg ctgcgaaggc
      301 ggcagggatt attgatgccg aaaaaattga gtttgtgtgg gcttcgacat ccgataagaa
      361 aatcacctta aaccacacgg ataaagacgc gactatttcc gcggatattg tcagtggcag
      421 cgagggcctg ggctatatca atgcgctcaa tggcacgact tacttaaccg gtgataactc
      481 tgcctttagt ggtaaagtca aaattgagca aaatggcgct ttagggatca cccaaaatat
      541 aggtacagca gagatcaaca actgcgggaa attacacctg aaggctgacg atagcatgac
      601 ctttgccaat aagatttccg gcaacggtac aataagtatc gacagtggga cggtggagtt
      661 gaccggcaat aactatgcct tcagcggata tattgatgtt gcttctggtg ctgtcgcggt
      721 tatttctgaa gataagaaca tcggccgtgc agagctggat gttgatggca aattgcaaat
      781 taatgccaac aaagattggg tatttgataa cgatcttgaa ggtagaggca ttgttgaaat
      841 aaacatgggg aatcacgaat tctccttcga tgagtttgct tatacagact ggttccaggg
      901 ttcactggcg ttccagaaca cgacatttaa tctggaaaag aatgctgagt ttctgcagaa
      961 aggcgggatc actgcgggtc agggaagcct ggtaacagtg ggtaagggcg ctcactccat
     1021 tagcactttg ggattctccg gcggaaccgt tgattttggt gccctgacag caggtgcaca
     1081 gatgacagaa gggacggtca acgttagtaa aacgctggat ttgcgcggcg agggtgtgat
     1141 tcaggtttct gacagtgacg ttgtccgctc agtatctcgt gatattgact ctgcgttatc
     1201 gctcactgaa gtcgatgatg gtaacagcgc cattaagctg gttgatgcgc aaggtgcgga
     1261 agttctgggc gatgcgggca atctgcaatt gcaggataaa aatgggcaaa tcctctccag
     1321 cagtgcccaa cgtgatattc agcagaatgg gcaaaaagcg gccgtcggca cttacgacta
     1381 tcgtctgacg agtggggtaa acaatgacgg tctgtatatc ggttacggcc tgacccagct
     1441 tgatttacac gctaccgaca gcgatgctct ggtgctgagc tctaacggta aaagcgagaa
     1501 tgccgccgat ctcagcgcaa agattaccgg cagtggtgac ctggcattca gcagccagaa
     1561 gggtcagacc gtatcgcttt ctaacaaaga caacgactat accggtgtta ccgatctgcg
     1621 cagtgggacg cttttgttga ataacgataa cgtgttgggt aatacccatg aactgcgtct
     1681 ggcggcagag actgaactgg atatgaatgg tcacagccag accgtgggca cgcgcaatgg
     1741 cagcgccgat tcactgctga gcttaaatgg cggcagtctg acggttacca acgggggcac
     1801 ttcaaccggt tcgttaacgg ggagcggaga gctgaatatt cagggcggca cgctggacat
     1861 cgcgggcgat aacagcaacc tgacggcgaa tgtgaacatt gctaattcgg ctaatgtcct
     1921 ggtaagtcat gcgcagggat tgggtagcgc aaacgttgag aacaacggta ccctggcgtt
     1981 gaataatagc gctgaaaaaa gagcggctgc gtctgtgaat tacaccctgg gcggcaatct
     2041 gaccaacaac ggtacgctga tgaccggaat gtcaggacag caagctggca a
//
LOCUS       DADLSB010000315         2071 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.319, whole genome shotgun sequence.
ACCESSION   DADLSB010000315 DADLSB010000000
VERSION     DADLSB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.319"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            7..2071
                     /locus_tag="JN331_004284"
                     /pseudo
     CDS             7..2071
                     /locus_tag="JN331_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          777 a          363 c          380 g          551 t
ORIGIN      
        1 tacatcatgt acaaatcatt atttactatt atcattctac tgtatagcgc cagttcatat
       61 gcaaaaagcg aatcccctcc cgcaagcact ataactttca ttgcacaaat cgatcatgtt
      121 gagctaaata aaacagcatt atctcatgat atgaaactcg ttcttgccga tcgtttttat
      181 ttcaaaacca gtaatccttg cgaaacaaat tcaatttctg ctcgaccaga atcaatggat
      241 attactgaca aagcctatgc cgacaaatta aaaaaattag aaactccttt tcttgatgat
      301 cgctatttct ttatgaccat caatcagtgt gacggtaatg gcactcccat gctgacagac
      361 ataaaagtat gtacaacaga attatgtggt gctgaatata caaagaaaat gtctactgta
      421 tggctcgatg aaactttaaa accaactgct aaacgtctgg caatttattt tattcaatct
      481 cctttaccct atgacgagga gaaaaaacaa tggaaagtca ccggatggta tatctcatca
      541 actgataatc agtcttcaac taaacagatt gcatttgaag gatatacaga cacagataac
      601 ttctccgatt ctaaatttat ttcttcattt aaatcctggt ttgaaagtgg aaagataaaa
      661 aacattgcta actataatcc aaaaggagaa agagatggaa aatacgaaag ttattacaat
      721 agcaatggta acgtctatga atctctaagt ttttctaatg gtcttatcaa cggtgagttt
      781 attacatatt atgaaaatgg ggccattgaa agtaaacgtc attttaataa cgacaaaatt
      841 aaagatggtg aatgccctca tttttatgaa tgatggaaaa atcaaacagc aacatagcta
      901 tctgaatagt aaactcgatg gtcctgcata tgaatattat cctgatggca aactcatgca
      961 ggaaaatttc tatcagtaaa gtgtgttgat tggaaacgat accagttact accaatccgg
     1021 aaaaataagc tctatacaca atagaaattc ccgtggtcag tatgatggag ctaacgaacg
     1081 ttatagtgaa gagggtaagt tactgtctaa atccgtctat aaagacggaa aacaaatttc
     1141 tgttcaaacc tggtatgaaa atgtgcagaa agaagaggag aaacattttg atgagcaagg
     1201 ccaattaaat ggtctggtta agcaatggta taaaaatggt aatcttgcaa aatcacaaaa
     1261 ctataaacat gacatactcg atggtaacag tgaagagtgg tacgaaaatg gcatccctga
     1321 aagtttgtac ccttataaga atggaaaaac cgacggcatt gccaaatcat ggaataagta
     1381 tggtaaatta acctattcaa tcgaatacaa aaatggcgta gagaatgggg catatcgtaa
     1441 ctggagcaaa aacacaggta aactaaccaa agaaaccctg tacgtcaatg gtatcagaca
     1501 gggagtggaa aaagagttta acgatcgcac aggtaaacta ttaacctcca cgcaatatgt
     1561 taataataaa agacatggta cagaggaaac ttatgatcaa aacggaataa aatatattac
     1621 atgctatcaa aatgatcaga aattatcgtc tttagacaat ccaacacaaa taaaagataa
     1681 tgcaacaaca ggcgattcca gtgctcaatt tgcattaggg aaatacgaat tcatctgtgc
     1741 aaatatagat gaagggatta aatggttaac caaatctgca gagcaaaaaa acactgatgc
     1801 gatatacttt ttagctacag cctacaaagg caatggtata cctgcaaaca atgaaaaata
     1861 cctggcatat ctgcaacaag ctgcaacact gggcagtagt aatgcccagg cggaaattgg
     1921 ctatttatat ctcatcggaa aagaactacc acagaatctt cctgatgctg gtgtctggtt
     1981 caaaaaagca gctgcacaag gtaattttgc cgcacatttc tatctgggaa ggatgtatca
     2041 gaatggtgac ggcgttgaga gaaatatgga a
//
LOCUS       DADLSB010000316         2042 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.320, whole genome shotgun sequence.
ACCESSION   DADLSB010000316 DADLSB010000000
VERSION     DADLSB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.320"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(219..383)
                     /locus_tag="JN331_004285"
     CDS             complement(219..383)
                     /locus_tag="JN331_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262208.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1285..1569
                     /locus_tag="JN331_004286"
     CDS             1285..1569
                     /locus_tag="JN331_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7262209.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1557..2042
                     /locus_tag="JN331_004287"
     CDS             1557..2042
                     /locus_tag="JN331_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7262210.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          526 a          426 c          585 g          505 t
ORIGIN      
        1 caaaatgatg gacattattt ttgtggaacc ggaggtaaca gaccagacgg tttaaatgag
       61 ccggttactt taaacgggtg atggacagca cctcaaaagt tcagatggat tatatttgtc
      121 tgcaataccc cgggtatttt cgatttgcga aatggatgga acgacttgct caggggatag
      181 ctgatggagt tactgaagta ccgaaggacc actaaaaatc acaggccggg aatttcggcg
      241 tttccggcgt aggtgtgcca cgaatgcatc cagtggcgtg atgttggatg caatgctgta
      301 tgagagatga ggggcggcgt ccagacgcag ccttcaaacc gccttgagct gctgccgtta
      361 agagcggtgg tggtgagcgt caaggttaaa aggcgcagaa catgtgtcag gtaggtgttg
      421 cggagatgaa cgaaagtgaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg
      481 aaaatgtgct atctacgcag cgcattacga gtccagcaga tatctggtac tggctgggcg
      541 gcatccggca tataggcagc atgaacgcaa cacaggctct cgtacggaac gtgggaacct
      601 gtcgtctcga tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga
      661 tgcgagacac aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga
      721 gcgaaggggg tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc
      781 atgagtcagc caaagccgtt cgaggtctct aagtatgctg tttgaaaagc ataccagcgg
      841 gtcaaagcta atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt
      901 accaatcgaa gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg
      961 cccgcaaaca caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa
     1021 ttcgtcacga ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg
     1081 ttcaacggca agagccgtat gacgtgagag tgtcacgtac ggttccgtgg gagcgtaggg
     1141 gggcggtgcc cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa
     1201 atgccgctga cgcggcatct gtaatctgtt acgttgtgta tatcatttgc tatatattgt
     1261 caatatgcta ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag
     1321 ctcaatctca gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt
     1381 cataagtcgc gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt
     1441 ctcgaccggc gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt
     1501 gatgctccgg ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg
     1561 gatgtgtaac tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagcg
     1621 gtgaagctgt acttgatgac tggttaaagc agaaggggct aaaaaatcag gcaatcggag
     1681 ccacccggac ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac
     1741 tggccactgg tagtgtcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag
     1801 accccattcc tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag
     1861 ggcttggcgc ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata
     1921 tcggtgtgcg tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc
     1981 atcatggttt taagccatca aaaactcagg tgcaaacatt gtttcttaaa ctccctcaat
     2041 ag
//
LOCUS       DADLSB010000317         2038 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.321, whole genome shotgun sequence.
ACCESSION   DADLSB010000317 DADLSB010000000
VERSION     DADLSB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.321"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            464..1150
                     /locus_tag="JN331_004288"
     CDS             464..1150
                     /locus_tag="JN331_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAY7262211.1"
                     /translation="MSELTAKALSDSRSFENAILERDMRMKKAGWIVAALAGLLLVFA
                     LAALILLLPLKTTTVELYTLDRQTGRIERVTTVGEDKLDTSEVLNLSQTASYVKRREG
                     YNYFALQQDYRETQMFNSDEVNKEYLDWFNSPDAPDAVFQQAAYVVTVEVLSNVPSEG
                     TGSSMVEMLRIKRTIRKVKDNTETYDYWTVRMTYRYFPQKKMTASEREVNPFGFIVTS
                     YQRFKEKSDE"
     gene            1143..2036
                     /gene="virB9"
                     /locus_tag="JN331_004289"
     CDS             1143..2036
                     /gene="virB9"
                     /locus_tag="JN331_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HAY7262212.1"
                     /translation="MNKVLMCALLLTAASAWGAATPRGSAYDSRMQNIPYNSQNTTVI
                     NTRAGYITTLVFDADEEVITATAGFPQGWTITPDRNRVGISPAPVKQPVTDADGKTVQ
                     KIFTPTQQDWQTNLFVTTTKRDYSLMLNVIDNDREAGNLAFVVRFSYPDDTRRQNDAA
                     RLARQQELQAAQEKERIQFALKKSTAPRNWNYTRRVAAGSESITPDFAYDDGRFTYLG
                     FSPQKLIPSPTVIVGGSEQVITPTFSTEGNYRVMVLRSLSPRFVLRYGSQVVGIENGS
                     YGKVTVANGSTVSPDVKLESK"
BASE COUNT          445 a          649 c          593 g          351 t
ORIGIN      
        1 ataagctggc cgaggcgctt gccggtgcat ccgcacaggg tgccgctcag gcacttgccg
       61 cgtcgggagt aggttcagcc cgtcaatccg cctcctccgc agccggtgcg gtgggggata
      121 aagcctcaca gaaccttaac agcaccggca gcgcaacggc ctcgtctgcc tccacggcct
      181 cccgcgccac cggcgatgcg cgcagccgca gccgcgccgc cgttcagcgg atgcaggaac
      241 gctaccagag ataacccagt ctgactgaac tgcgggacgc cgcaacggcg tcctgcggct
      301 tcgtcacgtc tttaccacag gaaaaacacc atgaaatacg gcatcacgct gctgatgctg
      361 tgcctgcttg caggctgcgc acagcgaaac acccaacttc cgcccgtgtc gggcaacccg
      421 gagccggtca attccccggc cattattcag gagttaaccc cccatgtctg aacttaccgc
      481 caaagccctg agcgactcgc gctcgtttga gaacgccatt cttgagcggg atatgcgcat
      541 gaaaaaggcg ggctggatcg tggcggcgct cgccggtctg ctgctcgtct tcgcccttgc
      601 cgcccttatc ctgctgctac cgctcaaaac caccaccgtg gagctgtaca cgctcgaccg
      661 tcagaccggg cgcatcgagc gcgtcaccac cgtcggcgag gacaagctgg acacctccga
      721 ggtgctcaat ctctcgcaga ctgcgtccta cgtgaagcgc cgcgaggggt acaactattt
      781 cgcccttcag caggactaca gagagacgca gatgttcaac agcgacgagg tcaacaagga
      841 atacctcgac tggtttaaca gcccggacgc gccggacgct gtgtttcagc aggcggccta
      901 tgtagtcacg gtggaagtcc tctccaacgt accgtcagag ggcaccggct ccagcatggt
      961 ggagatgctg cgcatcaagc gcaccattcg aaaggtcaag gacaacaccg aaacgtatga
     1021 ttactggacg gtgcgcatga cctaccgcta tttcccgcag aagaaaatga cggcctccga
     1081 gcgcgaggtg aacccgttcg gcttcatcgt caccagttac cagcggttta aggagaaaag
     1141 cgatgaataa ggtactgatg tgtgccctcc tgctgaccgc agcgtcagca tggggggcgg
     1201 cgacaccgcg cggcagcgcc tatgacagcc gcatgcagaa catcccctac aacagccaga
     1261 acacgacagt gattaacacg cgggcgggtt acatcacgac gctggttttt gacgccgacg
     1321 aggaggtgat caccgccacc gctggttttc cgcagggctg gacgatcaca cccgaccgta
     1381 accgcgtggg gattagcccc gcgccggtga agcagccggt aacggatgcg gacggcaaaa
     1441 ccgtccagaa gattttcacc cccacgcagc aggactggca gaccaacctg tttgtgacca
     1501 ccacaaagcg cgactacagc ctgatgctga acgtgatcga taacgatcgc gaggcgggaa
     1561 atctggcctt tgtggtgcgc ttcagctacc ccgacgatac ccgcaggcag aacgacgcag
     1621 cccgcctcgc ccgtcagcag gagcttcagg ccgcgcagga gaaagaacgc attcagtttg
     1681 ccctgaagaa atccactgcg ccgcgcaact ggaactacac ccgcagggtg gcggcaggtt
     1741 cggagtcgat caccccggat tttgcgtatg acgacggccg gtttacctat ctcggctttt
     1801 caccgcagaa gctgatcccg tcgccgaccg tcatcgtcgg cggcagcgag caggtgatca
     1861 ccccgacctt cagcaccgag ggtaactacc gggtgatggt actgcgctct ctttctccac
     1921 gctttgtgct gcgctacggc agccaggtcg tggggattga aaacggcagt tacgggaaag
     1981 tcaccgtggc gaacggcagc accgtctcac cggatgtgaa actggagagc aaatgatg
//
LOCUS       DADLSB010000318         2026 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.322, whole genome shotgun sequence.
ACCESSION   DADLSB010000318 DADLSB010000000
VERSION     DADLSB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.322"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..663)
                     /locus_tag="JN331_004290"
     CDS             complement(<1..663)
                     /locus_tag="JN331_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY7262213.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSG"
     gene            complement(691..2022)
                     /locus_tag="JN331_004291"
     CDS             complement(691..2022)
                     /locus_tag="JN331_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7262214.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT          554 a          596 c          453 g          423 t
ORIGIN      
        1 accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga gaataaagcg
       61 gatcgccgca aacgggactt cattgacgaa cttcaggatc tcagccagcg tcgttctgga
      121 aagcaccgtc agcggagact cttgctcgcc ctctgccaca cacgcctgct gggtaaacac
      181 cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga tggtgacaca
      241 cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat tgcaaggttc
      301 ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg caattgcctg
      361 cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta acgcccccag
      421 cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc ccagaccgtt
      481 cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt ccagttctgc
      541 cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca gcgccggttt
      601 tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa
      661 catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca gagaaacaca
      721 aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt tgtgcaatgc
      781 cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga aaatcgggct
      841 acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc aggccagcaa
      901 aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct cggcaggcat
      961 cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac
     1021 gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca ctttcaccca
     1081 cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc
     1141 gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa
     1201 gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt tatgccgcgc
     1261 cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta acgtctggat
     1321 aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca ccagcagccc
     1381 cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg acacgcccag
     1441 cgcagcgact accagcagct tggtcagcac catgccggtc gaaattttga ataacaattt
     1501 ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat aaaagggact
     1561 gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac tatcgttggt
     1621 gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga gcgcacctaa
     1681 caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat ctttacattc
     1741 tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg ccgggtaacc
     1801 aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca ccgggagaaa
     1861 aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg tgtcagtact
     1921 gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac ccgccggggt
     1981 cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatt
//
LOCUS       DADLSB010000319         2020 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.323, whole genome shotgun sequence.
ACCESSION   DADLSB010000319 DADLSB010000000
VERSION     DADLSB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.323"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            278..1378
                     /gene="ompC"
                     /locus_tag="JN331_004292"
     CDS             278..1378
                     /gene="ompC"
                     /locus_tag="JN331_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7262215.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1542..>2020
                     /locus_tag="JN331_004293"
     CDS             1542..>2020
                     /locus_tag="JN331_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY7262216.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSL"
BASE COUNT          629 a          402 c          391 g          598 t
ORIGIN      
        1 tattagcagt aattttgtgg ggatccctca ggtctacggc ccggcagtta atcagataaa
       61 ttccgacaat attaaaaaat aaatttcaaa taccagtaat tcaccttata tctcatttcg
      121 acttcgatca catatcgata aataatatta tttcatctat ttttctcatt gtttttaatc
      181 accattctat tttaaaaaca gtatgtcatt tataaatgac gccgtttaaa attcgtttag
      241 aaaatacgtt taacgataat taaaaggatt ctgttaaatg aaattaaaaa tagttgcggt
      301 ggttgtaact ggtttgttag ctgcgaacgt agcacacgct gccgaagtct ataacaagga
      361 tggtaataaa ctcgaccttt atggcaaggt taccgctcta cgttatttta ctgatgataa
      421 gcgtgacgat ggtgataaaa cttatgcccg tctcggcttt aaaggagaaa cgcaaatcaa
      481 tgatcaaatg attggttttg gtcactggga atatgatttt aaaggctata acgatgaagc
      541 caacggctcg cgcggcaaca agacccgtct ggcctatgct ggtttaaaaa ttagtgaatt
      601 tggctctctg gactatggtc gtaactacgg tgtcggctac gacattggtt catggaccga
      661 tatgttgcca gaatttggtg gcgatacctg gagtcagaaa gatgtcttca tgacataccg
      721 taccaccggt ctagcaacct atcgcaacta cgatttcttt ggcttaattg aagggctgaa
      781 ctttgccgcg caatatcaag gcaaaaatga acgtactgac aacagtcatc tttatggtgc
      841 tgactacacg cgtgccaacg gtgacggttt cggtatctcc tcaacttatg tttatgatgg
      901 ctttggtatc ggtgcggtat ataccaaatc cgatcggaca aatgcgcagg aaagagccgc
      961 tgctaatcct ctcaatgcct ccggtaagaa tgcagaacta tgggccacag gtataaagta
     1021 tgatgccaac aacatctact ttgcagctaa ttacgctgaa acattaaaca tgaccaccta
     1081 tggggatggt tatatctcta acaaagcaca aagttttgaa gtggtggcgc aatatcaatt
     1141 cgacttcggc ttgcgcccat cactcgctta cctgaaatcg aaaggcagag atctgggccg
     1201 ctacggcgac caggacatga ttgagtatat cgacgttggt gcgacgtatt tcttcaacaa
     1261 aaatatgtcg acctatgttg attataaaat caacctgatt gatgaaagcg actttacccg
     1321 tgccgtagat attcgcaccg ataacatcgt cgctacgggc attacctatc agttctaacg
     1381 tcctctcctg accagccctt ctctgggctg gtttttgatt taagtgctgc tgtatgactc
     1441 cttaagtaaa aatgagcaat ttttgatatt tctggtcatt cacctcaatt aatattattc
     1501 catatcctcc ggggacatct tactataata tttattgatg agtgattatg accaatatta
     1561 atacagcttg tgtaaaaaat aatgccagtt atcaacttaa taacgcatta cctaacaaag
     1621 aaaccatctc cagcaatttt tgtgaacgac tagaacaatg gggtaataaa tcggttaata
     1681 atggtgaaga aagagcaatt gccgtggagc gaattaaaga agcttacaat tcgaacatgg
     1741 catctcttga tttgtcatat cttgatttga gtgaattacc gcctatcccc tcgacagtta
     1801 atacgttaaa tctggaaaac aattgtctaa cttgtcttga cttagctgac aatgccagtc
     1861 tcgttaatat caacctcagc gttaataaaa ttaacacgat aacctttcca aaagaaccaa
     1921 aactggaaaa catttatatt gatcataaca atctggaaag tttagattta aaaaatcagc
     1981 attcattggt taatctggaa gcgcaaaata atagcctaaa
//
LOCUS       DADLSB010000320         1994 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.324, whole genome shotgun sequence.
ACCESSION   DADLSB010000320 DADLSB010000000
VERSION     DADLSB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.324"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..145
                     /locus_tag="JN331_004294"
     CDS             <1..145
                     /locus_tag="JN331_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262217.1"
                     /translation="REIDAIARKYVSLKYDGSIASDFSTMKCIDFIHGNELDELISRS
                     VKK"
     gene            259..744
                     /locus_tag="JN331_004295"
     CDS             259..744
                     /locus_tag="JN331_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAY7262218.1"
                     /translation="MSYDIFLEIDGIDGESMDDKHKNEIEVLSWRWNIHQESTMHAGS
                     GLGSGKVSVTNLSFEHYIERASPNLFKYCASGKHIPKAVLTMRKAGGNPLEYLKYSFT
                     DLIVAMVSPSGSQGGEIASRESVELSFSTVKQEYVVQNQQGGSGGTITAGYDFKANKE
                     I"
     gene            812..1345
                     /gene="tssJ"
                     /locus_tag="JN331_004296"
     CDS             812..1345
                     /gene="tssJ"
                     /locus_tag="JN331_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HAY7262219.1"
                     /translation="MNKWRNPGGWLCAVALSFVCLLSGCSIDSDDVPDLMSQRLDLVI
                     KASDKVNPDNQKKAAPIEIRVYELKNDAAFTAADWWSLQDNDKAVLADDLLHRDSFIL
                     RPGEEKKLRHALNKETTTLGILAGYRNLGKSVWRATYKIPEPPEKSWYSRFMPKKKVE
                     LDAGIEQSAIVIKERDK"
     gene            1349..>1994
                     /locus_tag="JN331_004297"
     CDS             1349..>1994
                     /locus_tag="JN331_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HAY7262220.1"
                     /translation="MSWNNRVVWSEGQFLLPQMFQQQERYLEHVIHYRSLPLSPFFWG
                     FNEYGIGSEALNIGKLVLTKASGVFADGTPFNAPGHTPLPPPLTILPEHLNQLIYLAV
                     PIRTPNSEETSFDNNPESLARFSVHENEIRDANSLGRGAQLLQLSHLRLRLLPEKAVT
                     DAWIGLPLTRITALHPDGRVDIDKELIPPVANYQASTLICTWLSWINDLIKMRAD"
BASE COUNT          559 a          445 c          501 g          489 t
ORIGIN      
        1 tcgtgaaata gatgcgatag cgagaaaata tgtatcatta aaatatgatg gttctatagc
       61 atcagacttc agcactatga aatgtataga tttcattcat ggaaatgaat tggatgagtt
      121 gatttccagg agtgttaaga aatgaaatat attcaatcct ttgttctgcc gattccatat
      181 gtggaagagt gttgtaaagt aatggtttat ttgtaagcgg ataaaccaaa attaatttaa
      241 ataaaaaagg tatgaaatat gtcttatgat atttttctgg aaattgacgg cattgacggc
      301 gagtcaatgg atgacaaaca taaaaatgaa attgaggtgt tgagctggcg ctggaatatc
      361 caccaggagt ccaccatgca cgcgggtagc ggtctgggat ccggtaaggt atccgtaact
      421 aatctttcat ttgaacacta tatcgagcgc gccagcccga acctgttcaa atactgcgcc
      481 tccggcaaac acattccgaa agccgtgctg accatgcgca aagcaggcgg caacccactg
      541 gaatacctca aatacagttt tactgatttg atcgtagcaa tggtttcgcc cagcgggagt
      601 caggggggag aaatagcttc tcgtgagtca gtcgaactct ctttcagcac cgtgaagcag
      661 gaatacgtgg tgcagaacca gcaaggtggc agcggcggca ccatcaccgc aggctacgac
      721 ttcaaggcca ataaagaaat ttaagcagta cttttccggc ccggcaaata gccaggccgg
      781 tttttattat ttgattttaa aggaaatcac agtgaataaa tggcgtaacc ccggtgggtg
      841 gttatgtgcg gtagctttgt cttttgtctg cctgctttcc gggtgcagca ttgacagcga
      901 cgacgtgccg gacctgatgt cacaaagact cgatctggtg attaaggctt ccgataaggt
      961 gaatcctgac aatcagaaaa aagccgcgcc catagagata cgcgtttatg aactgaaaaa
     1021 tgacgcagct ttcacggcgg cggactggtg gtcccttcag gacaatgaca aggctgttct
     1081 tgctgatgat ttacttcacc gcgacagctt tatcctgcgt ccgggcgagg agaaaaaatt
     1141 gcgccacgcc ctcaataagg aaactaccac gctggggatc ctcgccggat accgcaatct
     1201 gggaaaatcg gtctggcggg ctacatataa aattcctgag ccaccggaaa aaagttggta
     1261 cagccgcttt atgccgaaga aaaaagtcga actggatgct ggcatagagc aaagcgccat
     1321 agtgattaaa gaacgggata agtaaattat gagttggaat aaccgggtgg tctggagtga
     1381 aggacaattt ttgctgccac agatgtttca gcagcaggaa cgctatctgg aacatgtcat
     1441 tcattatcgc agtttgcctc tttcaccttt tttctggggc ttcaatgagt atggaattgg
     1501 tagtgaggcg ctgaacatcg gaaaactggt acttacaaag gcttccgggg tatttgctga
     1561 cggtactcct tttaatgcgc cgggtcacac accactccca ccgccgctga ccatccttcc
     1621 agaacatctt aaccagttga tttatctggc tgtgccaata cgtacgccga acagtgaaga
     1681 aaccagcttc gacaataacc ctgaatcact ggcacgcttt tcggtgcatg aaaatgaaat
     1741 ccgtgatgcc aattcactgg ggcgcggcgc gcaactgctg caacttagcc acctacgctt
     1801 gcgactatta ccggaaaagg cggtgacaga cgcgtggatc ggcctgccgc tgacgcgcat
     1861 cacggcgctg catcccgatg gtcgggtcga tatagacaaa gaattgatcc ctccagtggc
     1921 gaactaccag gccagtacgc tgatatgtac ctggctatcc tggattaacg atctgataaa
     1981 aatgcgtgca gatt
//
LOCUS       DADLSB010000321         1982 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.325, whole genome shotgun sequence.
ACCESSION   DADLSB010000321 DADLSB010000000
VERSION     DADLSB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.325"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(131..1585)
                     /gene="ospC2"
                     /locus_tag="JN331_004298"
     CDS             complement(131..1585)
                     /gene="ospC2"
                     /locus_tag="JN331_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY7262221.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          630 a          340 c          315 g          697 t
ORIGIN      
        1 caggattccc ggggcgttca ccacatgcaa tgtgttgttg caggggaacg gtctgcacca
       61 tcccctatat cgatcaatat aacaaaaatg acaacgtcag attcagggca caacctaaat
      121 atattttagg tcacctccca aatcgtttgc ctgataccgc tccatttttt aataaaaaat
      181 caattgtttt tgaatctttg cttttcattg cattgtcaag catagtgtcc ccactatttg
      241 ctttttgaaa tcttttgttt acatcaacca atccattatt aataaaatat tctagcactt
      301 tatagttggc actatcttca ctaagtaaat actcaacatc atacagctca caatacatat
      361 tgttgttcat tttctccatc tctgcgacat cttgttttgt aaaattaaat ttagatagta
      421 aataaagtgc catatcagat tttgcattgc ctagagaata agcaagagct tgatgagcca
      481 tttttttatc agtcacctta ttgttaattt cttcataatt ggatgcttta atggcatcct
      541 ccaaagatat atctctgagt cttatctttt tgaagttatc tgtgcttagc attcttggta
      601 tatgatactc aagctgaaac acaaagttta taattctatc cagactaaca ggatcaatgt
      661 ttttattata acaaaactct cggaatcttt gatctttact ctttcttata aaatcaataa
      721 gatgcaaccc aattccaagc ctcatatctt tagtattaaa tattggcaca tcagtttttc
      781 cctgattacc atgcacatac ctgctaactt catccacaac ctctttaaag ttatctaaga
      841 aaaatgattg gtgctcatgg taaaaaacag gtggaatagc attatcaaag tggtcggtta
      901 atgtcatata tccatatgga gagtcatgat caatgatata cgcattagca ccaaaatcca
      961 cagtatgatg tgttttattt agtgggagtg tttcttgatg attggaaatt tcaaccccaa
     1021 aaaaaacaaa atcgtggtta gctatttctt caatatcctc agagaatgtg tttttatatg
     1081 tttgtatatt ctcagactta attttatcaa aggacttaat attaagagta ttatttgaca
     1141 ggttagcgtt actttgatgt ctgaagttaa aatcagaatt aataataata gagagcaagt
     1201 gacgttcatc cgaagaaaga ggatgagttt tttgtattat ttcagaaaga tagtgtctgg
     1261 acaccttatc gaggtgctca agtgaccgaa atgatgaccg tgctccagat ggcgcttcaa
     1321 tagaaagatt taatgatttg aaactggtcc cttgagagaa cattttacta taactttgcg
     1381 ctgctataga cttccttaaa aagcttttca cagtatcagc acagtgatct aaatgttttt
     1441 cacttatgcc agaagaggat gagtttgtta cgcatgatat gtttttttgt aatgcttttg
     1501 tatctttgtt cggatttatt tttccatcaa caagatcaat attgttctga acattaatat
     1561 gatttactgc ttcaggtatt ttcataaaac atcccccatt atgtgcgttt ttatctgcag
     1621 ttatttagaa taatattatc taacttaata ttttcttgta tgtattataa cgaacatatt
     1681 ttaatatatt gtacgatttt cactctgaaa tcccctgagt ttaatcacac ttcctttact
     1741 gaaaggaatt attgcaacag tattatttgt ttttggatat atatcttgca ccttgttatg
     1801 tagttaatgc tcagaatatg atagataaaa tagagacgca gaacagggca tgctcataac
     1861 atgatgataa cataaaaaat tagtaacaat aaatgaaaga atcatgggcg ctcaacaaaa
     1921 agagagctaa atagctgcgc ggaatagtag gctgtgtccc ttaatcatct gagttatagt
     1981 aa
//
LOCUS       DADLSB010000322         1929 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.326, whole genome shotgun sequence.
ACCESSION   DADLSB010000322 DADLSB010000000
VERSION     DADLSB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.326"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            463..1512
                     /locus_tag="JN331_004299"
     CDS             463..1512
                     /locus_tag="JN331_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7262222.1"
                     /translation="MYNNKIDSNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKFGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
BASE COUNT          626 a          326 c          356 g          621 t
ORIGIN      
        1 tctgcttttg ggaggcatgg gtcactcagg ccaaatgaca tccgacggtt tcacgatgaa
       61 ctacggcaaa agaatatcag agccctcatt cctccccgaa agggagcggg ctctggtctg
      121 gtgagtatgc agatcgtaaa ggctatggcc atggtgcgag agtggcagct atgccagaaa
      181 gagtgtgtat tgcctgagac tctaacctga tgcagagacg attgtcttga acctgattta
      241 ttcaacaaag ccgctggcag gatgttacga aagttatcgc ggccgcaggc tgatttgata
      301 tcgatactat cggtggtact gcggaagtaa atgcctatat agtgcgactt tgaaaaataa
      361 taaattaata ttgaactcgt tgattaataa tcataaactt tcaggcattc atatttgcaa
      421 tgagtgtttg aaaatcacac aaaactggag agcctacttt aaatgtataa taacaaaatc
      481 gacagcaaca gaaaaacaag gcatgattgg gttgatgctt taaaatttct cggtattttt
      541 gctatatatt taggtcacct tggtttgggg gctggcaaat tgtatccttt tgtttttagt
      601 tatcacgtcc cactattctt tttcgctgct ggttttttta ctatcaaaaa aaatgactta
      661 tctattttcg attatatcaa gtcaaagttt tacagattaa tgattccata tttcactttt
      721 gcatttacta tattaataat aaacacaatt aactcaggtg agacgattga ttatatatat
      781 agccacattt atgatataat atatggtgtt agaaacaacc aatttgtagg aacaatatgg
      841 tttataaact gcctttttgt tataatagca attgatgcaa tctttaaaga aatagtcaag
      901 aacaatatag tgattttaat catatcacta ttatcgttca tgttgtcaca aacagtgctc
      961 aatcataacc cattactaga tcctcaatgg ttttggaaca tagactccgc tatggcatac
     1021 tggtggttat tgcctctcgg tagatgtatg tttttagaat taacacgtga caggtttttt
     1081 ggaaaatcta aatttggttt cattgtcttc tcaattaccg cgataatgag tgcgtatcaa
     1141 ctccttaacc agaaaccttt gctttttaag attatctcaa tattcaatgc agacataata
     1201 tcaagtagtt atattcaagc tattaatact attatcacca cggtaggttt gataatattt
     1261 aatattttta tagcaaaaat aatttgtggg aatgacttta ttgtaagagc tggaagaaat
     1321 actcttaaca tatgtggaat ggagtatatc accaaattat ttatccctat ggctttagca
     1381 atcataggct tcagcgtgac cattccaaat cctatctgcg caatcatata tacctgtatt
     1441 tgtgtatatg tatcagataa aattggccat tggctatcga ggacagtcgg tgggccattt
     1501 ttgataaaat gataaaaagg cgcaattaat tgcgcctttt tatagatata tagcgcatat
     1561 ttatgctcag attatactgt taaaattaat gcgcaaatgt tacggttttc tttcttggca
     1621 actgaaggga atgttcgaag tgcaaacata aaattacccc ttcactgaac agatattcag
     1681 tcggccaaca catgagaaag aaatacacca gatgaattaa aatatcatat actcattaat
     1741 caaagaaata aaagcataaa cagtaggtgg tgacgctgat aaactggcta taaacaacca
     1801 ggaaggtgat gagctatcgc aatataattt attggtgaat tatttgaagc cgattgataa
     1861 caccagtgct caaaagactt cccccggtaa ccataagtag ctaatatcta gtacagtttc
     1921 gatatctat
//
LOCUS       DADLSB010000323         1927 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.327, whole genome shotgun sequence.
ACCESSION   DADLSB010000323 DADLSB010000000
VERSION     DADLSB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.327"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1208)
                     /gene="pdeC"
                     /locus_tag="JN331_004300"
     CDS             complement(<1..1208)
                     /gene="pdeC"
                     /locus_tag="JN331_004300"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY7262223.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVPVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDV"
     gene            1636..1917
                     /locus_tag="JN331_004301"
     CDS             1636..1917
                     /locus_tag="JN331_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262224.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          553 a          424 c          427 g          523 t
ORIGIN      
        1 tcgacatcaa gaaatgcatg ctcagtcact tcaaatttaa tttgctgtgg acgcaccgcg
       61 tattgctctg ttttctgatt gattcgcgct atcaaccgtg acgtatgaaa atcggaggcc
      121 gacaggttaa tagaaacata gcgatcggca tgtgttgcca ggtacgcgcc cagatcgcgg
      181 aagacattat caataacata atcagttatc tgttctatca tcccctcctt ttctgccagc
      241 ggaataaact ctgccggatt cattatttgc ccctgctcac caggccaacg taacaacgct
      301 tcagcgccga tacatttttc tgttttgata tcgattattg gctggtaata aagacaaagt
      361 tgatgttttt cgagggcgcg ttgcaattta cgtttgggag ataaatagtt ttgtcgaata
      421 cgtagccaga gtaatagcag aacaagactc cccaaaatac cggcgggcaa cgcaaaaata
      481 agatgattat aaaaatgggt tataagacgt tgatatgaag tcgcaacaat ggctgcaatt
      541 gggcgttttg tcgaataaac tgtcgcatat aaatagccat ttctttgtac ggttaaatcc
      601 ttcaaatgaa tcagcggaga aaacgttgct gccgaggcct ctttgcttaa cgagaaaaag
      661 gttttcgtca ccgtatcata cacaccccat tgcaatgtcg ggtcatcaga catcacttca
      721 ctccagaaga gagggttgat aaccggcaca taatttcccc gctgcatata ggtcatttta
      781 tagccagaga aaaaaggcgt atcgcggtaa taatagatag aaacgttagg ttcacgctta
      841 taatcggccg gtgcaatcgt atagccgttt acaggcgcta tcagcgatga gcataaaaaa
      901 tggttatcac gggcatagat caattcatta atataaagat agccacgaat aatattcaac
      961 attcgctttt gatgggctgg agtgcaaact tgcccctgat agcgttcagc tgcatcgctc
     1021 actaaatctg cctggcgaat gaccagctcg gatttatcca gtgccagttg agcaaaggtt
     1081 cggagctgat tattcacttc tgattttgcc caaaggaagg caatccatag cgaaagaatg
     1141 atgggaaaga gaactaaaaa gataatgccc ggcaacgcca gtaattggtg tcgtgcacga
     1201 tgactcattg ctatcaattt cctgcctgtt tatacccggc cattgcaagg tgaaaccctg
     1261 gctaccgacg tctttagata aatcttacgg tataaatagc acttttgcct gatttgaaat
     1321 tgataccttt ttaatcgtca cctagatgaa aataattcat tgatattcaa aattaatttt
     1381 ctacaggaat acagaggcgg cgggaagata tgcccttaat gccactgaaa aaccacataa
     1441 tctgtacagc aataaaagtc acggcctaat atggctaaca aacaatcaat tgtgatatag
     1501 ttcacaaaat taatgaaaca aacagagtgt ttcatttttg tgttctgcaa gcccgcgata
     1561 gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg tccagggcat cggctctttt
     1621 cagaggaatt gtttgatggc taccctcacc actggcgtgg ttcttcttcg ctggcaactt
     1681 cttagtgccg taatgatgtt tctggccagc acactcaaca tccgttttcg tcggtctgat
     1741 tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg tttccgcctg ttggttcgca
     1801 atggggttgc ttggcatcac aatggcggac atcaccgcta tctggcacaa catcgagtcg
     1861 gtgatgatag aagagatgaa tcagacaccg ccacaatggc caatgatttt gacctgatac
     1921 gtagaag
//
LOCUS       DADLSB010000324         1920 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.328, whole genome shotgun sequence.
ACCESSION   DADLSB010000324 DADLSB010000000
VERSION     DADLSB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.328"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..585
                     /locus_tag="JN331_004302"
                     /pseudo
     CDS             1..585
                     /locus_tag="JN331_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(626..1621)
                     /gene="pgl"
                     /locus_tag="JN331_004303"
     CDS             complement(626..1621)
                     /gene="pgl"
                     /locus_tag="JN331_004303"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY7262225.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFSVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYSVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          494 a          513 c          453 g          460 t
ORIGIN      
        1 gtgcggattc tggaacaagg cacgaataat tgcatggaat cggtgctgtg taacgaatca
       61 gactttggca taaacatgaa caacgtcaca aattcatcca ttgattttac cccactggtc
      121 aacgagccat tcgtgctggc ctgtcggcgt gaccatccat tggccaaaaa gtagcttgta
      181 gaatggcagg agttagtggg ttacaaaatg attggcgtac gttcctccag cggcaaccga
      241 ttgttgatag aacaacagct agccgacaag ccctggaagc tggactggtt ttacgaagtg
      301 cgtcatcttt cgacgtcatt aggactagtg gaggcaggac tggggatttc ggcgcttcct
      361 ggtctggcaa tgccccatgc gccctattct tcgattattg gtatcccact ggtggaaccc
      421 gttatacgtc ggacattggg gattattcgc cgcaaagatg ccgtgctttc tccggcagct
      481 gaacgctttt ttgccttgct gattaatttg tggactgacg ataaagacaa tttgtggacc
      541 aatatcgtcg aacgccagag acatgcgtta caggagataa gttaatacac cggcgcagga
      601 gagactgcgc cggttaaatc agcggttagt gtgcgttaac caccacccac attggtccct
      661 gcccgaccgc atagcggcct ttttcatgca gtagcccctg ctcgccaaca atttcgtata
      721 ccgagatgtg gtgagatttt tgcccagcgg caatcagata cttgccgctg taatcaacat
      781 tgaagccgcg cggctgggtt tccgttggct ggaagccttc tttactcaac acgctgccat
      841 cttccgaaac gctgaaaacg gtaatcagac tggcggtacg gtcgcaggcg tataaatggc
      901 gaccatccgg ggtgatatga atatcagccg cccaacgggt gtcggagaag ttttccggca
      961 tcatatccag cgtctggaca cattcgatat tgccgtgcgg atctttcagt tcccagacat
     1021 ccactgagct gtttaactca ttgacgctat acgcatattg ttcgtttgga tggaatacca
     1081 tatgacgcgg gccggcccct tcaacggtag tcacttccgc agggtcctgc gccaccagat
     1141 gaccatcatc gctgaccgta aacaggcaaa tgcgatcctg ctttaatgcc ggaacccaca
     1201 gcgtacggtt gtccggtgag atattggcgg aatggcaacc gtccagcccc tcgaccacat
     1261 cgacgacgcc cactggcagg ccatcttcca gacgcgttac gctcacgtta cccgcattgt
     1321 aagaacctac aaagacaaac tgcccctggt gatcggtgga aatatgcgtc ggactacccg
     1381 gcagcacaga ctctgcggca aaggtcagtg cgccatcgtc cggggcaata cgatacgcca
     1441 ggacgctaaa ctcagggcga acaccaacat agagataacg tttgtccggg ctgaccacca
     1501 tcggctgcac ctgccccggc acatcgacaa cctgtgtcag cgtcagtgcg ccttcatgat
     1561 tcaggttcca gacgtgaatt tgctggctct cagggctggc gatataaact gtttgcttca
     1621 tgaacacacc tcaaaaatga taaatccctt atctttcata cgcataacaa cacttactgg
     1681 ataaaaaaca gacaacaaaa cacgataaaa tacaatatcg cacatcaatt aaatccataa
     1741 gttagcactc catttcgaat ggcatattac taaaatatta gtcaccgaca cgaatttgac
     1801 aatgccacag atattttcgc gactattcag ggagtatgac tctccccgct tttgtttacc
     1861 tgtatcaacg accgtaaaca ctatgccata acaacttctg gccaacttcc cattcgctca
//
LOCUS       DADLSB010000325         1791 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.330, whole genome shotgun sequence.
ACCESSION   DADLSB010000325 DADLSB010000000
VERSION     DADLSB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.330"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            461..1543
                     /locus_tag="JN331_004304"
     CDS             461..1543
                     /locus_tag="JN331_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HAY7262226.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
BASE COUNT          566 a          364 c          336 g          525 t
ORIGIN      
        1 acatgcccca tcacatcctc aacatcaaag tattagagcc ttgcaaacca ttatgcgtct
       61 cagtttgtct catctatctt acgcaaaaaa aatagaacta atatcaagcg gcgctatatt
      121 gcgtattaaa taaaaccacc tcaattacat taataaatct aaataaatat catgcaaata
      181 atttccatgg ttatttttag gctatttaaa ttaaaaaaat aaattatgag tcaatcataa
      241 ataacaacct ttaatataat ttgactacca aatttatatt cgttcacaaa atagtcatgc
      301 attgtgtgca atagaaacag ttcagataaa aatagggatc tacttcacaa tttaaatgag
      361 aaactaaaac ttacatcttg aaataatctc attgattgga tgaatatttg tagtgcagtg
      421 acatcatttt taaataatag ttcaaataaa agggctcacg atgaaaaaat taacagtggc
      481 aatttctgct gtagctgcat cagtactgat ggcgatgtct gctcaggcag cagagattta
      541 caataaagac agtaacaagc tggatctgta cggaaaagtt aatgctaagc actacttctc
      601 ttctaacgat gcagatgatg gcgacacaac ttatgttcgt ctgggcttca aaggcgaaac
      661 tcaaatcaac gatcaactga ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg
      721 tgctgaatct caaggttctt ccaaagataa aacccgtctt gccttcgctg gcctgaaatt
      781 cggtgactac ggctccatcg attatggccg taactacggt gtagcatacg atatcggtgc
      841 atggaccgac gttctgccag aattcggtgg cgatacctgg acccaaacag atgtgttcat
      901 gactggtcgc accacaggtg ttgcaaccta tcgtaacaac gacttctttg gtctggttga
      961 tggtctgaac tttgctgctc agtatcaggg taaaaatgac cgcactgacg taactgaagc
     1021 taacggtgat ggtttcggtt tctccactac ttatgagtat gaaggattcg gtgtaggtgc
     1081 aacctatgct aaatctgacc gcactaatga tcaggttatc tacggtaaca acagcctgaa
     1141 tgcatctggt caaaatgctg aagtatgggc agctggtctg aaatatgatg cgaacaacat
     1201 ctatctggct accacctatt ctgaaaccca gaacatgact gtttttggta ataaccatat
     1261 tgccaacaaa gcacaaaact tcgaagtagt tgcacaatat cagttcgact tcggtctgcg
     1321 tccgtccgtt gcttacctgc aatctaaagg aaaagacttg ggtgcgtggg gtgatcagga
     1381 cctgattgaa tatattgatg taggtgcaac ctattacttc aacaaaaata tgtccacttt
     1441 tgttgattac aaaatcaacc tgattgataa gagcgatttc acgaaagcat ctggcgttgc
     1501 taccgatgat atcgttgctg taggattggt ttaccagttc taatctgatt actcaaagat
     1561 atgttgtggg aggctttgcc tccccaacat ataagtggcg ccctcaagcc actttcctta
     1621 gaagcactat cttgcttctg actatataaa ccttctgtta tatattaccc tttattttct
     1681 gggacgtttc aacgccccat ttttaataat actcagtgaa caattaacgt atcaattaga
     1741 tttaataaca acgatatccg tacctaacct gatatctaat gtcataagca t
//
LOCUS       DADLSB010000326         1776 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.331, whole genome shotgun sequence.
ACCESSION   DADLSB010000326 DADLSB010000000
VERSION     DADLSB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.331"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            23..259
                     /locus_tag="JN331_004305"
     CDS             23..259
                     /locus_tag="JN331_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY7262227.1"
                     /translation="MLSQYAASPRLNALINSFNAALSPDSFISDFYGLIWNIDTAEKY
                     GLDVWGKIVGVSRRLTVKDDFNYLASASPGWTPR"
     gene            244..606
                     /locus_tag="JN331_004306"
     CDS             244..606
                     /locus_tag="JN331_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY7262228.1"
                     /translation="MDTPVMDDPCPFNQAPFYNGKSDTRTVDLSDAVYRRLILMKAMS
                     NITDCSVPDINRMLRFMFGKKRRAYVLNNGGLRMSYIFESALSLAELAIIQSSGALPS
                     PPGVYVSVVLKEPRNEGQ"
     gene            593..>1776
                     /locus_tag="JN331_004307"
     CDS             593..>1776
                     /locus_tag="JN331_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7262229.1"
                     /translation="MKASDNPRQLAVPFASAGDKNSIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDTLSGGLTFENDSTLAWIRNTDWAKIGFKNDADSDTDSYM
                     WFETGDNGNEYFKWRHRLAGGRVKDLMNLKWDTLNILVNAVINGCLGIGTTNALGGSS
                     IVLGDNDTGFKQNGDGLLDVYANGQRVFRFQNGSIQSNKAVNVTGRVTPSDYGNFDAR
                     YQQRNGGVQDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVY
                     YRP"
BASE COUNT          451 a          397 c          483 g          445 t
ORIGIN      
        1 cgtgcagaat gtggctgcca ctgtgctttc gcagtatgct gccagccccc gactcaatgc
       61 cctcattaac agctttaacg cagcgctttc ccccgacagt tttatcagtg atttttatgg
      121 tcttatctgg aacatcgata ccgcagaaaa gtatggtctt gatgtctggg gaaagattgt
      181 gggtgtcagt cgccggctga cggtaaagga cgattttaat tacctggctt cagcgagtcc
      241 aggatggaca ccccggtaat ggatgatccc tgtccgttta atcaggcacc gttttacaac
      301 ggaaaatcgg atacccggac tgttgacctg tctgatgctg tataccggcg gctgatactg
      361 atgaaagcca tgtcgaacat tactgactgt tccgttccgg atattaaccg gatgctgaga
      421 tttatgttcg gaaaaaaacg ccgggcttat gttctgaata atggtgggct gaggatgagt
      481 tacatctttg agtccgcgct ctcgttggca gaactggcga ttatccagtc gtcgggtgca
      541 ctgccatccc cgccgggtgt ttatgtttca gtggttttaa aggagccccg taatgaaggc
      601 cagtgataac ccccgccagc tggcggtccc ctttgcgagt gccggagata aaaacagtat
      661 tccggacaag gcgacgcagc agaccagaga gagcggtaat gctgcgtatg attcaggttt
      721 tcctccggtg accatgacag cggtctcagc gggaggtata ccgccacacg gcaaggattt
      781 taacggtctg atgtacgata ttaccgcagc aatacggttc gcccaggctg gcggtttgta
      841 cacgtataat gccggttttg cgggggccat tggtggatat gcaaaaggag ccattctcgc
      901 cggagtcgca acaacagcgg tctggctgaa taccacagac gataacctga ccgatcctga
      961 aggctccgac agtgcgggct gggtaaatct tcttgaggat ccgaaaagga tattcctgcg
     1021 gcagaagaac aatctgtcag accttcagaa taaagggacg gcacgggata atcttcaggt
     1081 ttacagtaaa gagcagtcag atcaacgcta tgttcatcgg gaaggcgata ccttgtccgg
     1141 tggacttact tttgaaaacg actcaaccct tgcttggatt cgaaatactg actgggcaaa
     1201 gattggtttt aaaaatgatg ccgacagcga tactgattca tacatgtggt ttgaaacagg
     1261 tgacaacggc aatgagtatt tcaaatggag acaccgcctc gctggcggcc gggttaaaga
     1321 cctgatgaat ctcaaatggg atacactaaa tattctggtt aatgccgtca ttaatggttg
     1381 tcttggaatt ggtacgacga atgcgttagg tggaagttca atcgttttag gagataacga
     1441 taccgggttt aaacaaaatg gcgatggtct gcttgatgtt tatgcgaatg gacagcgtgt
     1501 atttcgtttc cagaatggca gtatccagag taataaagct gtaaacgtta caggacgggt
     1561 aacaccgtca gactacggaa actttgatgc ccgttaccag cagcgaaatg gtggcgtgca
     1621 ggatgtgcgg tatggttatg aaatgtatta caaccctggc agcaacaccg tttcatggac
     1681 gtttcgctca ccttcgggac acgggctgtc agggatatcg atatcggata ccggccgtaa
     1741 ctcagcggat aacgtcaacg gtgtgtatta ccgacc
//
LOCUS       DADLSB010000327         1770 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.332, whole genome shotgun sequence.
ACCESSION   DADLSB010000327 DADLSB010000000
VERSION     DADLSB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.332"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..198
                     /locus_tag="JN331_004308"
     CDS             <1..198
                     /locus_tag="JN331_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262230.1"
                     /translation="EMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYR
                     QLTDEVLALRLSENGSQLHHS"
     gene            406..675
                     /locus_tag="JN331_004309"
                     /pseudo
     CDS             406..675
                     /locus_tag="JN331_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            672..784
                     /locus_tag="JN331_004310"
                     /pseudo
     CDS             672..784
                     /locus_tag="JN331_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            927..1517
                     /gene="ospG"
                     /locus_tag="JN331_004311"
     CDS             927..1517
                     /gene="ospG"
                     /locus_tag="JN331_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY7262231.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          514 a          365 c          440 g          451 t
ORIGIN      
        1 gagatgctgg agaatgagta ccctcagagg gtggctgacc ggctgaaagc atcaggtctg
       61 agcggtgatg cggatgcgga gagggaagcc ggtgcacagg tgatgcgtga gactgaacag
      121 cagatttacc gtcagctgac tgacgaggta ctggccctgc gattgtctga aaacggctca
      181 caactgcacc attcataatc acatcgcata aaactgtcag cgccagtgat aggacaggtg
      241 aaacctgtca atacgtaccg gcttacgccg ggcttcggcg gttttactcc tctgccatat
      301 gcaacaacgg agtgccgcct tacatgccgc tggcgcggca tatcctggtg acgatatctg
      361 aacagttata tactgtgtat atacgtggta atgacgaggt ggcaaatggc acaggttaat
      421 atgagtgtaa gaatcgacgc agaactgaag gatgctttta tggccgctgc aaaaagcatg
      481 gaccgtaatg gctctcagtt aatccgggat tttatgcgcc agaccgttga acggcagcac
      541 aattcttggt tccgtgacca ggttgcggca ggccgtcagt aactcgaatg cggtgatgtg
      601 cttccccatg acatggtcga atcctctgct gctgcatggc gggatgaaat gagcaggaag
      661 attgctgata aatgatggag atgttctgga ccatgctggc catccaggac aggaagcgca
      721 ttcgtagtac attgcggagc agaacctgat ggccgccatt gaaccggacg aacggattgg
      781 ttattcagcc agcagcctgg cagggcagcc gtataaaggc cgcaacggtc gtgtggaagg
      841 aacatctgga ccacacaagg tagcgtgtaa cgttattttg tgtgaaaatc tcttgtgatt
      901 tttaaatatc gtaacaaagg tatgacatga aaataacatc taccattatt caaacacctt
      961 ttccatttga gaataataat tctcatgctg gcatagtaac ggagcccatt ctcggtaagt
     1021 taataggtca ggggtcgaca gcagaaatct ttgaagatgt gaatgattca tctgctttgt
     1081 ataaaaagta tgatcttatt ggcaaccagt acaatgagat tctggaaatg gcttggcaag
     1141 aatctgagct ttttaatgct ttttatggcg atgaagcatc cgttgttata cagtatggcg
     1201 gagatgtgta cctccgaatg ctgcgcgtgc ctgggactcc ccttagtgac attgatacag
     1261 ctgatatccc tgataatata gagagccttt atctacagtt gatatgtaaa ttgaatgagt
     1321 tgagtataat ccattacgat cttaatacag gtaatatgct gtatgataaa gaaagtgaaa
     1381 gtttattccc aatagatttt cgcaatattt atgctgaata ttacgctgca accaaaaaag
     1441 ataaagagat tatcgaccga cgattacaaa tgcgtacaaa tgatttttat tcgttattaa
     1501 acaggaaata tttatagccg tatttgttga tgctataagc cagaggctct tctatgagaa
     1561 tatggggacc attccctcgc gatattaacg taatatctta attgtatgca ccgccagaat
     1621 gcgttcagtg cgatcacggc agcagacagg taaaaatagc aacaaaccac ccgaaaaacc
     1681 gccgcgatcg cgtatgataa attttaacca catgcatacc tgtgcagcaa ggcgaatcac
     1741 gcacggattt ccagccagag acacctgaaa
//
LOCUS       DADLSB010000328         1758 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.333, whole genome shotgun sequence.
ACCESSION   DADLSB010000328 DADLSB010000000
VERSION     DADLSB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.333"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..715)
                     /gene="ipaH9.8"
                     /locus_tag="JN331_004312"
     CDS             complement(<1..715)
                     /gene="ipaH9.8"
                     /locus_tag="JN331_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7262232.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPQI"
     gene            complement(977..1072)
                     /locus_tag="JN331_004313"
                     /pseudo
     CDS             complement(977..1072)
                     /locus_tag="JN331_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1108..1413)
                     /gene="ccdB"
                     /locus_tag="JN331_004314"
     CDS             complement(1108..1413)
                     /gene="ccdB"
                     /locus_tag="JN331_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY7262233.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1415..1633)
                     /gene="ccdA"
                     /locus_tag="JN331_004315"
     CDS             complement(1415..1633)
                     /gene="ccdA"
                     /locus_tag="JN331_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY7262234.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          486 a          364 c          390 g          518 t
ORIGIN      
        1 aaatctgcgg gccgtggtag tccggcgaag aggttaatct ttgcagggct tgcagagcat
       61 gggatgataa tgggttatca ctaatatgta ttgaacattc attcctcaga ttaagaatgc
      121 tttccgggat atgacttatc tgatttctat caaaaaaata ctctctgaca acgggattat
      181 ttccctcaga aaatgctggt agctcagtga ggaaattacg ggtcgctctg agatttttaa
      241 gagcctgtgg gagcgatggt aaggagacta tttcgttata gctgatattc atagtcaata
      301 acgaatcggg taaagctggc aggttttcca gctcattgtg ttgtacctga agtgactcca
      361 gcgcaggagg tagcacgggc aattctgata atttattgct ggcggaatat aattttttta
      421 gcgtaacagg cagttcaggt aggttagtta attgattata tgatacattg agcagcgtta
      481 tctgagctgg taagttgtca ggtagcgagg acagatttaa acggtccagt cgaagttcat
      541 cggaattatt gataagacat tctttaagtc gggagacagc ctcatcacgc tcttcaccgg
      601 ggagcgcttg tttttcccat ttatcccatg ctgaaaagta atcagcatat gtaccggaaa
      661 tagtgttata aaaagaattt tggggcaatg aaaagttatt atttatcggt aacatgatgg
      721 gcatccagtt aaagccggtg gacaaatggc tttgttaaaa gaaataagta ggagttttta
      781 tgaagttcat tccatgaaat gaacttcagc acttttttat tataataagg ataaacaaaa
      841 ttggctgtaa aaaaacgata ctgtttcagt taaccgtgaa tggcctgttt gcaccgtatt
      901 gctcttgttt ttcggttagt taaacatcgt acttcatatt tgaacggtaa gcggctcgtc
      961 agaaccgtat tgatatttac tgagaggtca gatcaacttt ccagggcaac agatcgcgta
     1021 cccggtttgc cggccagtcc tggatatgct caatgacgta acgcagccat tttgtcaacg
     1081 ccacgatttt tgaccctagc ctgacattta tattccccag aacatcaggt taatggcgtt
     1141 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cgaagatcgg
     1201 cacactggcc atatcagtgg tcatcatgcg ccagctttca tccccgacat gcaccaccgg
     1261 gtaaagctca cgggagactt tatctgacag caggcgggca ctggccaggg ggatcaccat
     1321 ccgtcgtccg ggcgtgtcaa taatgtcact ctgtacatcc acaaacagac gataacgact
     1381 ctctctttta taggcgtaaa ccttaaactg catttcacca gtccctgttc tcatcggcaa
     1441 aagagccgtt catttcaata aaacgggcga tctcagccat cccctgctga ttttcagcct
     1501 gccagcgttc cgcacgcaga cggcgggcct ctttttgcat ggccgtattt accaggcctg
     1561 agatattcac gtttgcggat ttaagtaact gatagctgtc actgtctata gtgacagtaa
     1621 tacgctgctt catgataagc cctcaaaaac atacttatga tatgtatatg agtatatatt
     1681 aatggtacaa accatcaata tccataaaaa gtacaccatt aattcgctat aagtttttaa
     1741 tacaattttt tctgctgc
//
LOCUS       DADLSB010000329         1744 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.334, whole genome shotgun sequence.
ACCESSION   DADLSB010000329 DADLSB010000000
VERSION     DADLSB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.334"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..849)
                     /locus_tag="JN331_004316"
     CDS             complement(<1..849)
                     /locus_tag="JN331_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7262235.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIHSLTPFSLPMDLTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
     gene            complement(1238..1504)
                     /locus_tag="JN331_004317"
     CDS             complement(1238..1504)
                     /locus_tag="JN331_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262236.1"
                     /translation="MLTQTIFPCLPQKQENISLEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1613..1744)
                     /locus_tag="JN331_004318"
                     /pseudo
     CDS             complement(1613..1744)
                     /locus_tag="JN331_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          495 a          282 c          362 g          605 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattc gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc aagcttatct ggaaaggggg gaatgctgtc
      301 taatctgttt ccagaaacta ccagtaattt tagttccggt ggcaatagtg gcagaatatg
      361 caatcggtta ttatgaaccc gtaaatgagt tagattttca ggaagaaatg gtaattcttt
      421 tatcttgtta tcacccgcat taagctcttc taacgtctcg ggtaaggcag gtaagctttc
      481 cagttgatta aattgagcat gtattacctt aagcattggt ggtaaatcag ggagatgtgt
      541 taataggttt tttcttattt ccagtgttgt tatctgaggc ggtaaatctg gtaatgtttt
      601 taaatctaac tcagataaat ttaattctgc ctcttgattt tgtaaacata ttttcagcct
      661 attgaaggcg atattgcgtt gctcgcctgg ggtaccgttc ttttcccatt cactccaggc
      721 ttttaaataa aattcattac aagatggagt cagatccata ggtaaagaaa atggagttaa
      781 cgagtgtata ttgtttattt gatgcataat accagaaccg attgcctgta tattggttga
      841 tttaatcatt ataactagaa ttcttatcaa cgataagaat tctagtgaaa tagaatatag
      901 cttattaatt ttaaaacagt ttgctttgta ccctttctcc gggtattgct ttccccctaa
      961 gccgtctatg ggaaggagtc tgctgttatt cctgagaatg gtaattcagc cataaaaagg
     1021 tatttaaggt cattgcgtct ctccctgtac tcgaataatg gtgaagaaag catgatgaat
     1081 atatcacgat aacgttcagt acgtatcgag gataaatgga gttactcctc tctgaaatag
     1141 aattactgct ctattttttt ctgttttcag aagttattag taacaaaccc tgatatattt
     1201 ttttgaaaat ggagcttaaa cgagtctgaa attaacttca gaaatcagaa taccgttggc
     1261 tttgtttttc atcaattccg gcttcattta tctttgtcaa ccattgcatt tctgtagcag
     1321 tgaatttcaa cgttttcgtc cggttggcag caggtatttg taattcgtat atagctgctt
     1381 ttttattgaa aacagtatat ccatctgtag taacagtaga agataataat acaggatttg
     1441 atacttcaag gctgatattt tcttgttttt gtggaaggca agggaatata gtttgtgtaa
     1501 gcatattact tccttatgtt atattttggt aaaaaattta tttttttaca ttatagtcgg
     1561 ctaaatgaag tttactgtaa aaaaacgata ctttttcggg tggcgcttcc aatcagtaga
     1621 atatgtgttt ttcaatgaag gagccgatga cttttcgtgg atttccacgg aacgcgagaa
     1681 gcagattgtt ttacgtgcca gccgcttgat gcgggtgcgg agggtcaggt tgttacgttc
     1741 aaga
//
LOCUS       DADLSB010000330         1729 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.335, whole genome shotgun sequence.
ACCESSION   DADLSB010000330 DADLSB010000000
VERSION     DADLSB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.335"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..161
                     /locus_tag="JN331_004319"
     CDS             <1..161
                     /locus_tag="JN331_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7262237.1"
                     /translation="CRYEKASIILTSNKGVADWGEMFGDHVLATAILDRLLHAEYQRR
                     ELPVKRET"
     gene            655..1233
                     /locus_tag="JN331_004320"
     CDS             655..1233
                     /locus_tag="JN331_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262238.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="JN331_004321"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="JN331_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..1729
                     /locus_tag="JN331_004322"
                     /pseudo
     CDS             1584..1729
                     /locus_tag="JN331_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          563 a          304 c          398 g          464 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agaccggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatgg catattacca tgcaaattat agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataat
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggc
//
LOCUS       DADLSB010000331         1713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.336, whole genome shotgun sequence.
ACCESSION   DADLSB010000331 DADLSB010000000
VERSION     DADLSB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.336"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>1713
                     /locus_tag="JN331_004323"
     CDS             <1..>1713
                     /locus_tag="JN331_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY7262239.1"
                     /translation="KLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVV
                     FVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQH
                     ISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREF
                     RDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRL
                     AYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQ
                     LSLRAQQLSMVGNDLRYAGRFPVSREGSETVSDASGHVALNLPAADKPSRYLLTVSAS
                     DGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLR
                     LEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGT
                     VDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTLQRLND
                     TQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGE
                     LVNV"
BASE COUNT          403 a          446 c          469 g          395 t
ORIGIN      
        1 taaaactgga aggcgcatcc agcaatttca tctcgccgca gccgggtaat atttatattc
       61 ctctcggcca acaagagccg gggctgtacc tcgtcgaggc gatggtaggt gggtatcggg
      121 cgacgacggt ggtgtttgtt tccgataccg tggcgcttag caaagtgtca ggtaacgagc
      181 ttctggtatg gaccgcgggt aaaaaacagg gtgaagcgaa gcccggctca gagatcttgt
      241 ggactgacgg tcttggcgtg atgacccgcg gggtgaccga tgacagcggt actttgcagt
      301 tacaacatat atcgccagaa cgttcataca ttctgggtaa ggatgctgaa ggcggcgttt
      361 ttgtctccga gaacttcttc tacgaaagcg aaatctacaa cacccgcttg tatattttta
      421 ccgatcgccc gctatatcgc gcaggcgatc gtgtcgatgt taaagtgatg ggccgcgagt
      481 tccgcgatcc gttgcattca tcccccatcg tcagcgcccc ggcgaagctt tcggtgctgg
      541 acgctaacgg cagtctgttg caaaccgtcg atgtcacgct ggatgcgcgc aatggcgggc
      601 agggaagttt ccgcctgcca gaaaatgccg ttgccggagg ttatgagtta cgtcttgctt
      661 accgcaatca ggtctatagc agcagttttc gcgtggcaaa ctacatcaag ccacatttcg
      721 agattggttt agctctcgac aaaaaagagt tcaaaactgg cgaagcggtc agcggcaaac
      781 tgcaactgct ctacccggat ggcgagccgg taaaaaatgc ccgcgtgcag ttaagtttgc
      841 gcgctcagca attatcaatg gtcggtaacg atttgcgtta tgccggacgt ttccccgtgt
      901 cgcgggaagg cagcgaaacg gtgtccgacg ccagcggtca tgtggcgtta aatctccccg
      961 ccgccgataa accgagccgc tatttgttaa ccgtctccgc cagtgacggc gcggcgtatc
     1021 gcgtcaccac caccaaagag atcctcattg aacgcggcct ggcgcattac tctttaagta
     1081 ccgccgcaca atacagtaat agcggcgagt cggttgtgtt ccgttatgcc gcgctggaat
     1141 cttcaaaaca ggttcctgtt acgtatgaat ggttgcgtct cgaagaccgc acgagccata
     1201 gcggagagct accgtcaggc ggcaaatcct ttaccgtcaa tttcgctaaa cctggcaact
     1261 acaatctgac attacgcgat aaagacggct taattctcgc tgggttaagt catgccgtca
     1321 gcggtaaggg cagcacggcg catactggta cggtagatat cgtggcggat aaaacgctgt
     1381 accagccagg cgaaaccgcg aagatgctga ttactttccc ggagccaatt gatgaagcgt
     1441 tattgacgct ggaacgcgat cgcgtggaac agcagtcgct gctttcgcat ccggcaaact
     1501 ggctaacgct acaacgttta aacgataccc agtatgaagc ccgggttcca gtgagcaatt
     1561 cctttgcgcc taacatcact ttttcggtgc tgtatacccg taatggtcag tacagttttc
     1621 agaacgccgg gatcaaagtt gccgttcctc agcttgatat ccgggtgaaa acggacaaaa
     1681 cccattacca gcctggtgaa ctggtcaatg tcg
//
LOCUS       DADLSB010000332         1696 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.337, whole genome shotgun sequence.
ACCESSION   DADLSB010000332 DADLSB010000000
VERSION     DADLSB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.337"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..121)
                     /locus_tag="JN331_004324"
     CDS             complement(<1..121)
                     /locus_tag="JN331_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7262240.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILE"
     gene            449..524
                     /locus_tag="JN331_004325"
     tRNA            449..524
                     /locus_tag="JN331_004325"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:482..484,aa:Asn,seq:gtt)
     gene            complement(580..1515)
                     /gene="ldtA"
                     /locus_tag="JN331_004326"
     CDS             complement(580..1515)
                     /gene="ldtA"
                     /locus_tag="JN331_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7262241.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(1577..>1696)
                     /gene="cobT"
                     /locus_tag="JN331_004327"
     CDS             complement(1577..>1696)
                     /gene="cobT"
                     /locus_tag="JN331_004327"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="catalyzes the synthesis of
                     alpha-ribazole-5'-phosphate from nicotinate mononucleotide
                     and 5,6-dimethylbenzimidazole; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY7262242.1"
                     /translation="GAALAMSIIEAACAIYNNMGELAASNIVLPGNTTSDLNS"
BASE COUNT          449 a          428 c          372 g          447 t
ORIGIN      
        1 cttccagtat ggcaacctgc tggctgagcg ctggttgtgc gatatgcaat acttcagcag
       61 cctgggtcag gctaccaata tctacaattt ttacgaagta tttcaggcgt ctgaagttca
      121 tgttgcctcc ggtttttaag aatcggccca agtgccgcca ttacttacaa ccagtatgca
      181 agatgcctgc cagttctatt ttggcgttga tgcgcaagct aaccaactgt caaataagag
      241 attatgatag attcgtcatt ggcccttttt atcagcagtc gcattcacct gccctataaa
      301 aggaaggtat gcaccacgat ggttcattac ccaataagat tgaaagctca ccactttgtt
      361 gaaattggca gcaaacaaac aaaaaaaatg catttcaccc tttgacatca ccatgcactg
      421 ccattaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
      481 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaagcctg
      541 ctttcgagca ggctttttac tttctaatta ccaacgctct taaaacatct gtcttgaacc
      601 agaactaatt tgcacaggca ttcccgatcg atgttgcaac gcagcatttg cgcgatttac
      661 atcaacttct tgcccgttga taaacgcccg caaagatggg gttaccggca atggcacttt
      721 tcggtcagac tcatattctg cacgattgcg cgacagcggc tcatgaactt ccagccactt
      781 tgaaccatcc ggttccgttg tgtatttcac tggctgatca ataatttgta cacgagtgcc
      841 tacaggaaca ttatcaaaca gatatttgat atcgtcatta cgcagacgaa tacagccctg
      901 acttacacgg agcccaatac caaaattggc attggtaccg tggatggcat acaacctgcc
      961 aatataaatc gcgtacagcc ccatggggtt atcgggcccc gcaggaacaa atgcgggcaa
     1021 actctcccct cgtttcgcat attcgcgccg ggtgttcggc gttggcgtcc aggttggtgc
     1081 ttcttgttta cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc cagcctggcc
     1141 gataccaata ggaaagactt ccactgtatt actgtctggc ggatagtaat aaagacgcat
     1201 ttcagcgacg ttaaccacaa tccctttacg aacagtggcg ggcaaaatca gttgctgcgg
     1261 aatggtgagt tgtgagccag gcttcggcaa aaaaacatca gcgcccggat tcgcttccag
     1321 catattactt aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct gagtattgtg
     1381 atcaggaaca gttacagtaa gcgactgccc cactaaacgg ctaccctctg gaggtaatgg
     1441 ataagttacc gccaggctat tttggctggc aaaaagcaga gcaaatgagc aaagaatatt
     1501 tacacgacgc atcatgtccc tttcctaagt cgcgaatact atccgataag tatagctttt
     1561 atcagacttt tcgtttttag ctgttcaaat cagaagtcgt attccccggt agaacaatat
     1621 tactggcagc aagttcgccc atgttgttgt atatcgcaca agcagcttcg atgatggaca
     1681 tcgccagagc tgcacc
//
LOCUS       DADLSB010000333         1635 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.338, whole genome shotgun sequence.
ACCESSION   DADLSB010000333 DADLSB010000000
VERSION     DADLSB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.338"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(1..1005)
                     /locus_tag="JN331_004328"
                     /pseudo
     CDS             complement(1..1005)
                     /locus_tag="JN331_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481272.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(1340..1558)
                     /locus_tag="JN331_004329"
     CDS             complement(1340..1558)
                     /locus_tag="JN331_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262243.1"
                     /translation="MFKCNCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKV
                     DVLILIFGYINALFLGVFMAVMCIEYWR"
BASE COUNT          447 a          390 c          348 g          450 t
ORIGIN      
        1 gcactgcttt tgtactggaa agtgagattt ttagacagaa cgcgcttcgc ctgtaggctg
       61 aagatatacc ccagttcctc ttcagaatgg tgcacatcaa gatgagcatt atcagtagta
      121 cgaggcgacg tagcgaaccg gttgttatag gcttcaataa aggtcggcaa ccatgcattt
      181 gctgtttcaa tatcactgat attctgaagc cgcatttctt tgaccagcct gtcctgtagt
      241 gtctgattgg cgcgctctac ccgccctttt gcctgcgggc tgttggcatg gattggctcg
      301 atgcccagtg tctttatcgc acgagtgaac tgggtcagct caccttcccg ctctgggtta
      361 tttaccctga atatactgtg tctatcagag tagagagcga gcggtacgcc atgatcatta
      421 aggtaacccc ggagggtttc catgtaagcc cgggttgttt cagcaggcac aaaacgcaac
      481 gccatcaacg cactggtggc atcatcaatg aaaacgatca gtgtacatct ggggcctcga
      541 ttttcaaacc agtcatgagg tgagccatca atctggatca gttcaccgta agatggtcgt
      601 cgcatgcggc gttgatatat acgggcaatt ttacgacggc gttcacgcca taccccctct
      661 tctatcatcc actttctgag agtttcaacg gataaagata aaccgtgtat ctcgcgcaat
      721 ttttcgcacg caaacgtagg tccaaaatca gcgtaacggc ctttgaggag tgaaattact
      781 gttgctctga attcagtaga aaaggaatta ttaggacgct ttccacgtcg gtgggaaacc
      841 agaccagaag gcccttcatt tctataccgt tgcaccagac gtttaactag aaatgccaat
      901 gcgtgccgca gcttgttcct gagtaatatg tcgattaatt gactcctgaa taatctgaag
      961 tcggtggagt tccttatgac tcatcgtaac agtctctttg atcatgaaaa atcccccaga
     1021 gaattgtctg gggacatttt agaatggttc aaaggggaca ttacagcttg gtgttaacaa
     1081 tagcctgctt ctccttgcct ttcggcacgt aagaggctaa cctacatgtg tctggcatga
     1141 aattggcctc agattaatgt taagcgtctt gcaggacgcg taatgttaac tggggctttt
     1201 ctctacctgc cgttggtgtt cgtgcccgag gcagatagcc tcaagcaccc gcagtcattc
     1261 tacttaacta agatttcccc gcaaaccgtt tttgtccagc acagtaaata tccaactaaa
     1321 ccaatggagt tcgctgtatt taccgccagt attcaatgca catgaccgcc atgaacaccc
     1381 ctaaaaaaag ggcatttata tatccaaata ttaatatcaa aacatcaact ttttccatat
     1441 accttgctgt gaagatgatg ggcatacatg atgcgaacaa ccagaacgca acaaacaaaa
     1501 actgcaatgc gtttttcatt attcctccta caatcaatgt gcaattacat ttaaacacac
     1561 ctcaatttgg ccggacatat aaatatctaa accagaaaaa atcacttaca tagcgttaca
     1621 aactctttag tctaa
//
LOCUS       DADLSB010000334         1630 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.339, whole genome shotgun sequence.
ACCESSION   DADLSB010000334 DADLSB010000000
VERSION     DADLSB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.339"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1046)
                     /locus_tag="JN331_004330"
     CDS             complement(<1..1046)
                     /locus_tag="JN331_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY7262244.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1538..>1630
                     /locus_tag="JN331_004331"
     CDS             1538..>1630
                     /locus_tag="JN331_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HAY7262245.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIV"
BASE COUNT          480 a          270 c          346 g          534 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaggac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atcttaatgt actgtttttt tctttaaaaa aacaatacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct ccccccccga
     1501 cttttgacgc agtgtcttgt taacaggggg atttaccatg cttattggct atgtacgcgt
     1561 atcaacaaat gaccagaata ccgatctaca acgtaatgcg tttatttgtg ccggaagtga
     1621 gttgattgtt
//
LOCUS       DADLSB010000335         1616 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.340, whole genome shotgun sequence.
ACCESSION   DADLSB010000335 DADLSB010000000
VERSION     DADLSB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.340"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..196
                     /locus_tag="JN331_004332"
     CDS             <1..196
                     /locus_tag="JN331_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HAY7262246.1"
                     /translation="ALNQLLEARTQLANLQTYMDGKAGAENLINKLLQDPTLLKALAS
                     APKPVAQQESVLSGETGTAD"
     gene            221..>1616
                     /gene="tssC"
                     /locus_tag="JN331_004333"
     CDS             221..>1616
                     /gene="tssC"
                     /locus_tag="JN331_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HAY7262247.1"
                     /translation="MVNDTLLATGDLSAAQTTAYQDTEFDSLLDKAFRPKTPQAAKAV
                     EAAVQTLAQQALTNTVTVSDDAYKSIAAIIEQIDFKLTEQIKLILSHPEFQKLESSWR
                     GMEHLVYNTETDEKLKIRFMNLSKDELRKNMKRYKGIAWDQSPMFKKLYEAEYGQLGG
                     EPYGCIIADYYFDHTPPDVDLLGSIAKVAASAHAPFIAGASPSVLQMDSWQELANPRD
                     LIKIVTQNLEYAPWNSLRKSEDSRYIGLTMPRFLSRLPYGAKTNPVDEFDFEEDADGA
                     DHSKYVWSNAAYAMGVNINRSFKHYGWCTLIRGVESGGAVENLPCHTFPTDDGGVDMK
                     CPTEIAISDRREAELAKNGFIPLIHRKNSDYAAFIGAQSLQKPQEYYDPDATANANLS
                     ARLPYLFACSRFAHFLKCIVRDKIGSFKEREDMQRWLNEWIMNYVDADPVNSSQETKA
                     RRPLAAAEVVVEEVE"
BASE COUNT          436 a          389 c          395 g          396 t
ORIGIN      
        1 tgccctgaac cagctgctgg aagcgcgtac tcagcttgcc aacctacaaa cgtatatgga
       61 tggtaaagcg ggagcagaaa atcttatcaa caaactatta caagacccta cgttactgaa
      121 agctctggcc agtgcgccaa aacctgtcgc tcaacaggaa agtgtcttat caggtgaaac
      181 gggaacagca gactaactgc gattttatta aggaactttc atggtaaacg atactcttct
      241 ggcgactggc gatctctctg ctgcgcaaac cactgcctat caggacactg aatttgattc
      301 gctcctggat aaagccttcc gtcccaagac acctcaggca gcaaaggctg ttgaggcagc
      361 agttcagacg ctggcccaac aggctctgac aaataccgtc accgtcagtg acgacgctta
      421 taaaagtatt gctgcgatta ttgagcagat tgatttcaaa ttaactgagc agatcaaact
      481 catcctgagt catccggaat ttcagaaact ggagtcctcc tggcgcggga tggaacattt
      541 ggtttacaac actgaaaccg atgaaaaatt aaaaatccgc tttatgaatc tttcaaagga
      601 tgaattgcgg aaaaatatga agcgttacaa agggatcgcg tgggatcaaa gccctatgtt
      661 caaaaagctt tatgaagctg aatatggaca gttaggtgga gaaccttacg gttgtattat
      721 tgcggattat tattttgatc acacgccacc ggatgtggat ctgctcggtt ccattgccaa
      781 agtagcagcc tcagcccatg cgccgtttat tgccggggcc tctccgtcag tgttgcagat
      841 ggactcctgg caggaactgg ctaacccgcg tgatttgatc aaaatcgtga cccaaaacct
      901 cgaatacgca ccgtggaact cgctgcgaaa aagtgaggat tctcgctaca ttggcctgac
      961 gatgccacgt tttctctccc gtttgcctta tggtgcgaaa acaaaccctg tcgatgagtt
     1021 tgattttgaa gaagatgcgg atggcgcaga tcacagcaaa tatgtctgga gcaatgccgc
     1081 ctatgcgatg ggtgtaaata tcaaccgttc tttcaaacat tatggttggt gtacgttgat
     1141 ccgtggtgtg gaatctggtg gcgcggtgga aaatctgccc tgtcatacct tcccgaccga
     1201 tgacggcggc gtggatatga aatgcccgac agaaattgcc atctctgacc gtcgtgaagc
     1261 tgaactggcg aaaaatggtt ttatcccact tatccaccgc aaaaactctg attacgccgc
     1321 ctttattggt gcgcagtctc tacaaaaacc gcaggagtat tacgatccgg acgctacggc
     1381 caacgccaat ctttcagctc gtttgccata tcttttcgca tgttcacggt ttgcgcactt
     1441 cctcaaatgt atcgtgcgag acaagatcgg ctcgttcaaa gagcgtgaag atatgcagcg
     1501 ctggctcaat gagtggatca tgaactacgt cgatgccgat ccggtgaact cctcacagga
     1561 aaccaaagct cgtcgcccac tggcagctgc tgaagtagtg gtggaagagg tggaag
//
LOCUS       DADLSB010000336         1615 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.341, whole genome shotgun sequence.
ACCESSION   DADLSB010000336 DADLSB010000000
VERSION     DADLSB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.341"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            66..458
                     /gene="ydeI"
                     /locus_tag="JN331_004334"
     CDS             66..458
                     /gene="ydeI"
                     /locus_tag="JN331_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HAY7262248.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            713..1603
                     /gene="dgcZ"
                     /locus_tag="JN331_004335"
     CDS             713..1603
                     /gene="dgcZ"
                     /locus_tag="JN331_004335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HAY7262249.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSYMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT          454 a          312 c          387 g          462 t
ORIGIN      
        1 tttggcaatt ataaaaatag caaccagact taataagtca aaccaaacgc aattaaggag
       61 ttagtatgaa atttcagtcc attgtattag caagttttct tgtcatgcct tatgcgttgg
      121 cagatgacca gggcggttta aaacaggatg cagcgccacc gccgcctcat gcaatagaag
      181 atggctatcg cggaaccgat gatgcaaaaa aaatgaccgt tgatttcgca aaaaacatgc
      241 acgatggcgc ttcggtttca ctacgcggta atttgatttc tcacaaagga gaggaccgtt
      301 acgtttttcg cgataagagc ggtgaaatta atgtcgttat tcctgcggcc gtctttgatg
      361 gacgagaagt tcagccggac cagatgatca acattagcgg cagtctggat aagaaaagcg
      421 cgccggccgt cgttcgggtc actcatttgc agaaataaaa ataaattagc ctgatggcct
      481 gaactactgg gccattaggg tattgggtta tgctatttgt catcctttta tttacgataa
      541 tgcagaggtg atttacagaa ttaaacactt ttttatccgc tgattttgtt ttaaatcaca
      601 atgttatctc agagatgact cataacttcg gatttctatg aaaatattga cgtaattttg
      661 cttttctctc gttagaatag cgcgcacaag gaactgtgaa aaaggagtgg caatgatcaa
      721 gaagacaacg gaaattgatg ccatcttgtt aaatctcaat aaggctatcg atgcccacta
      781 ccagtggctg gtgagtatgt ttcacagcgt ggtcgcgaga gatgccagta agccagaaat
      841 aacggataac cattcttatg gactgtgcca gtttggtcgg tggattgatc atctggggcc
      901 actcgataac gatgaattac cttacgttcg gctaatggat tctgcccatc aacatatgca
      961 taactgtggt cgggaattaa tgctggctat tgttgaaaat cactggcagg acgcgcattt
     1021 cgacgccttt caggaggggt tgctttcttt tactgcggca ttaaccgatt acaaaattta
     1081 tttgctgacg atccgtagct atatggatgt tttgacggga ttgccggggc gtcgggttct
     1141 tgatgaatcc tttgatcatc agttacgcaa cactgatcct ctgaatcttt atttaatgtt
     1201 gttggatatt gaccgattta aattggttaa tgatacctac gggcatttaa tcggcgatgt
     1261 agtattacgc accctggcaa cttacttagc cagttggacg cgtgattacg aaacggttta
     1321 tcgctacggg ggcgaagaat ttatcattat tgttaaagcg gctaatgatg aagaagcatg
     1381 tcgtgcaggt gtcagaattt gccagttagt cgataaccat gccatcacac attctgaagg
     1441 gcatatcaac attaccgtga cagcaggtgt gagtcgcgca tttcctgaag agcctctgga
     1501 tgtggtcatt ggaagagcag accgggcaat gtatgagggt aagcaaaccg gaagaaatcg
     1561 ctgcatgttt attgacgaac aaaatgtgat taaccgagtt taagctccgt ttaac
//
LOCUS       DADLSB010000337         1610 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.342, whole genome shotgun sequence.
ACCESSION   DADLSB010000337 DADLSB010000000
VERSION     DADLSB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.342"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(251..1610)
                     /locus_tag="JN331_004336"
                     /pseudo
     CDS             complement(251..1610)
                     /locus_tag="JN331_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
BASE COUNT          572 a          229 c          282 g          527 t
ORIGIN      
        1 ttatgtaatt aggcggttaa actttaatat tcgcgttaag tttgggtaaa cgcttgtaaa
       61 tggtatcgct aattctttaa ttgactaata tttgaaacgg ttagtgtgat tgtatataaa
      121 tatttgagtg taaggtattg aaaggggcat tcagcccctc cgtctatttg tgattgcatc
      181 cattgatgcg cgtattattt tgagcttgaa aaagggaggc gctctttaat aataaataaa
      241 tctatcaaag ctaaatatta aatggaagcc accctttttt ctctgatagt tttattatca
      301 ccaaaccatt tatttcagtt atcgaatctg gattctgcca ggaaggtaaa ctcttcacaa
      361 ttggataaat cttctctatt tgttctttat ttgctggttt gcaattacag attccatagt
      421 aattcataaa tgcaaccatc ctaaaatagt tgccaccatc ccaccaaaaa aagattttcc
      481 aaaagtgtct gcgctcatgg agagcatatt tcctgagtcg tttgaaccat gtatatatat
      541 gtattttccg ttaagggaaa tgccttttgc ttgagatata tatgatattt ctttagctaa
      601 agagatatct ttctgtcttg caagagtatc attcaaaaat agatttttgg atattgaaac
      661 gctattaaat ataataaata aaaaaacatg tagtttaggt gttttatcga gtgaaaattg
      721 cttaagcaag aaaacaaaat tactgctact attggcatta aaacaaacag gcgaggtagc
      781 gcccctgagc caacaacaac gataaatata accggaagta tataaataaa aatgattgat
      841 gaaatcaaat aaattgattt tgtttttaat ttatatatta gggtgaatat cagaactaat
      901 aaaggaatca gtaaccactt gtataagttt aaaccagtag gaggattgtt atagaagtta
      961 tatatattat ctatggctga tttaacaagc catttaatat cgcttgcatt ttgtatgtaa
     1021 tttgagatga actcgttcga ttcaagcgaa aaataatgct tgatagcttt ggttaatagc
     1081 aggtaaatta aggtggataa agctatgagg gatagagata aacatgaaat ataaaaagac
     1141 tcacgaatat ttttctcttt tcgtattatc gaatttacct gcaacccaat gacgaatgca
     1201 ataatatatg ttacgaatgt ttggtagatt accattgaaa gaatggacag tactatacca
     1261 gaaaatatca caatcctatt tttttgcgag tgaaaaataa ttgctgatat cgctgccagt
     1321 agaaaagcaa ttcccaccgt atcagcttgg ttaagaaact ctaattgata ggatatctga
     1381 ggaaaggtaa taaatactaa catacctatc aataattcat aagattctag ctttagcgat
     1441 ctgcagatta taaatgctga agtgatgata aaaaataaag ctactaatgg tgttatataa
     1501 agtgaaaaag gctcagggag aaaataatgt cgcaaaaaag tgtgaaacca ccgtccaagc
     1561 gtgattgttt gataaaaatt atttgtaaac tcgccatcaa tattaagtgt
//
LOCUS       DADLSB010000338         1599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.344, whole genome shotgun sequence.
ACCESSION   DADLSB010000338 DADLSB010000000
VERSION     DADLSB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.344"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..399)
                     /locus_tag="JN331_004337"
     CDS             complement(<1..399)
                     /locus_tag="JN331_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HAY7262250.1"
                     /translation="MSRLVVVSNRIAPPDEHAGSAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYH"
     gene            complement(374..1174)
                     /gene="otsB"
                     /locus_tag="JN331_004338"
     CDS             complement(374..1174)
                     /gene="otsB"
                     /locus_tag="JN331_004338"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAY7262251.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(1341..>1599)
                     /gene="araH"
                     /locus_tag="JN331_004339"
     CDS             complement(1341..>1599)
                     /gene="araH"
                     /locus_tag="JN331_004339"
                     /EC_number="7.5.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Part of the ABC transporter complex AraFGH involved
                     in the high affinity transport of arabinose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAY7262252.1"
                     /translation="FSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILG
                     TVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
BASE COUNT          389 a          430 c          378 g          402 t
ORIGIN      
        1 gtgataatcg tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc
       61 cagcaacgca tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcaccagatc
      121 gagccgataa tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc
      181 aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg ttaccttttt tcaccttttt
      241 tagcggctga tcctcattcc ctgtttcacc actccagcca aaccacagtc cgcctgcggc
      301 tttcagtgcc cccagtatgc caacggcaag gccaccggca ctgccggcgt gctcgtctgg
      361 tggtgcaatc cggttagata ctacgactaa acgactcata gtcatcactc ctgttatttt
      421 ctcttttttg ttgtaatgcg gtggttatca tttcaagcca gctccagaca tccggcacac
      481 ccgccagtcg ccatgatgcc tgagttgcac ctgtgccaat ttttactgac attccgccca
      541 gtcggttaac gactgcgaag ccagattcat cggttaaatc atcgcccaga aatacgggcg
      601 ttcgcccgat aaaaggagct tcctgcataa aggctgcaat cgcttcacct ttactggtac
      661 ctctcggttt gatctcgaca acacactttc cctgctgtaa cgccatttgt ggccagatct
      721 gagtaatacg ttgcgctaat gtcattaatg cgtcttcatg ctgcggagcc tgacgataat
      781 gcagcgcaaa agccatccct ttcgcctcca gctccgcgcc gggatactga gcgatgaccg
      841 tatgcagttg cacgctaata tcacgcgcaa tcgcatccgg cagatgaacg atatgtgttt
      901 taccattgat gtcacggcgc tccgccccat gcacgcccgc taacgggaag cgataaggtt
      961 ttgccagtgc gtcaagctcc accattgagc gccctgatat caatgccaat gcaccatcac
     1021 ttgcggttgc cagtagctgt agtccttgca gaatattgtc aggcacgacg acctgatcgg
     1081 gatgcggttt gatttccgcc agcgttccat caagatcaaa aaaccaggca tatttcgcgg
     1141 atagttcagg ggtttcggtt aacggttctg tcacccggct ctcctccttc tcttttctta
     1201 ttgtgttgcg cagaccgcgc tttacagaca tgtaagtata gacagtgtga cggaggtcgc
     1261 catttgcaac gaagaacagc caaccgttgt ggcggctggc tgtggtggga aaaaacgtta
     1321 aattgttgtg gaaaaaagca tcagacagtg cgtttcgctt tttgcttgta acggtcgaag
     1381 atcaccgctg ccagcaggat taagccgcga accacgtact gcgcgaaagg agaaatatta
     1441 agcaggttca tggcgttttc cacggtgcct aaaattaaga tacccgccac cacatatgag
     1501 atttttccga tgccaccttt cagagaaacg ccacctaaaa cgcaggcgga gataacaata
     1561 agctcataac caatcgacgt cattggctgc ccactaaat
//
LOCUS       DADLSB010000339         1596 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.345, whole genome shotgun sequence.
ACCESSION   DADLSB010000339 DADLSB010000000
VERSION     DADLSB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.345"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            3..386
                     /locus_tag="JN331_004340"
     CDS             3..386
                     /locus_tag="JN331_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7262253.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(387..1295)
                     /locus_tag="JN331_004341"
     CDS             complement(387..1295)
                     /locus_tag="JN331_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7262254.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLIDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERPGFQVYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            1395..>1596
                     /locus_tag="JN331_004342"
     CDS             1395..>1596
                     /locus_tag="JN331_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HAY7262255.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYS"
BASE COUNT          423 a          354 c          381 g          438 t
ORIGIN      
        1 tggtgctggt ggcaattctg attatgctgc cgactctcgg catcggtgat atgaacaatc
       61 tctataaatg gctgttgaat cttaactcgg tagtgatgcc gctgcgttat ttatgggtat
      121 ttgttgcatt tattgcagtc gttcgcctgg cgcagaaata taaaccagaa tatgtcttta
      181 ttcgtaataa gcctctggca atgaccgtcg ggatttggtg ttttgccttt accgcctgtg
      241 cctgtttgac ggggatcttc ccgaaaatgg aagccttcac tgcagagtgg accttccagt
      301 tggcgctgaa tgttgcaacg ccgtttgtgc tggtaggatt aggactgata ttcccgctgc
      361 tggcgcgtaa agcgaatagc aaataattat tgtggtcggt gacgctgact ctggcagatc
      421 tgacccgttt ttacataatc gagaaatgcg cgcaagccgc aggacatata ctggcggttt
      481 gggtaataga cttggaagcc tggacgctct gtgatccagt cttccagtac acattctaaa
      541 cgaccagtat caaggtactc cttgatctgt tcatagagca gatacccgat accagcaccc
      601 atcaaaacgg cctccagttc agcatcaaca tcatccagaa tgatatttcc tgcgacagca
      661 atttccagct cctttgcaaa ttgccagtgg aaaggtttgc cgctgggata acgaaaaaca
      721 acacattgat gattaagcag atcatgagga tggcggggtt tgccatatct tgcaaagtac
      781 tctggcgttg ctgctacaca taatttcact ggtgggccga tcgccacgct gatcatatct
      841 ttttcgacga tgccactaag tcgaacgcca gcgtcaaagc cctgttggac gatatcgata
      901 agagaatcgt cagttgtgag ctctacttta atatcaggat attcacgtgt gaaaccaacc
      961 agtaaaggca ttaaaaatat acgtgcagct acccgggcgg cattgatttt gagcgttcct
     1021 gtcggtgtca gacgaaaatc attcatctca tctaacatga tctgaatttc gtcgaaagct
     1081 gggcgcaatc gttcatagag attagaaccc gcttccgtta acgatacgct tcgggttgtc
     1141 cgattgaata agcgaatttt aagacgttgt tccagtgttt taatactatg gctaatggcg
     1201 gacgaggata agcctaactc atcgcctgct gcacgaaagc tttgattacg ggccacggca
     1261 aagaaagtgg caaagtcaga catattcatc cgcattagtg aaaatccttc attaactcat
     1321 cctgaatcat cacgtttaca ggattatgcc gcctgcgtaa agtgcgctcc agaacttaac
     1381 gtggaggtaa aattatgcag tctgaacgta tttatttggt atgggcccat cctcgtcatg
     1441 attcattgac cgcaaatatt gctgatgcga tccatcagcg ggcaatggag cggaaaatac
     1501 aggtgacgga actcgattta tatcggcgta atttcaaccc ggtgatgacg tcggaagatg
     1561 aaccagactg gaagaatatg gataaacgtt attctc
//
LOCUS       DADLSB010000340         1588 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.346, whole genome shotgun sequence.
ACCESSION   DADLSB010000340 DADLSB010000000
VERSION     DADLSB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.346"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..206)
                     /locus_tag="JN331_004343"
     CDS             complement(<1..206)
                     /locus_tag="JN331_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAY7262256.1"
                     /translation="MDDIVNSVPSWTFTAIIAVCVLFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIP"
     gene            complement(233..862)
                     /locus_tag="JN331_004344"
     CDS             complement(233..862)
                     /locus_tag="JN331_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HAY7262257.1"
                     /translation="MNLFADHNTPYLFAISFVLLIGLLEIFALICSHMLSGTLDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLQEPEEGKIFTKGDKVLIICRLSATRYLVENNPWPQIL"
     gene            1131..1331
                     /gene="glgS"
                     /locus_tag="JN331_004345"
     CDS             1131..1331
                     /gene="glgS"
                     /locus_tag="JN331_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY7262258.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(1374..>1588)
                     /locus_tag="JN331_004346"
     CDS             complement(1374..>1588)
                     /locus_tag="JN331_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY7262259.1"
                     /translation="LDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRA
                     ELSANPAETVVEGPFSKDVILKITYN"
BASE COUNT          488 a          303 c          355 g          442 t
ORIGIN      
        1 gggattattt catgaaagat cggcatcacg attgcgccac cgctcatcac cactttttgc
       61 ccacctaaac cagtacgaac aaatgcttgc tctgccgaag cgcgacgata gagcctggcg
      121 aaaataattc caataataaa cagaacgcat acggcaataa ttgcggtaaa cgtccaggaa
      181 ggcacagaat taacgatatc atccattatt aaatttcctt ttgattaata aattacaata
      241 tttggggcca gggattgttt tccaccaaat atcgagttgc agaaagtcga caaataatta
      301 acactttgtc tcctttagtg aaaatttttc cttcttcagg ctcctgtagt aaataatgta
      361 tttggccaaa ttgatcggtg agttttcctt cgcagggatt acctgaagtg gcttgatgac
      421 cggtaattaa cgccatgcta ccaatatatt cttcttctgt aatagctgaa ctatggtcgc
      481 gtggaatcca gggcgcaact attttaccgg tataatgcac cgcaataatc gtaaacagta
      541 aactgacagg aaccacgaac aggttcgaca gtggtgattg ccagaccatg atgcaggcat
      601 gttgcaacag gatgccaata agaccaaaaa aaccagcgag taagcagaga acgacgagag
      661 ctggcaatct tccgatattg agataatgaa gtgcctggct aatatgacct gtggttatgg
      721 aatcataatg gtcaagatgt gcatcaagag taccggagag catgtggcta cagataagcg
      781 caaaaatttc taaaagaccg atcaacagta caaaggaaat agcgaaaaga taaggggtgt
      841 tatggtcggc gaataaattc atatgtcact ccaatgtcca tttagtgacg caatatttac
      901 cttggaatag tttccctgct tacatttgtc tttgttaata ataacacatg ttagagaagt
      961 tctggaagag aaagtatttt aatatggttt tttatttttc actaaaaagt gtgatcgggg
     1021 acaatatatt tacgcacgtt atgtttaaag gcactacact gattggggaa tactgaaatc
     1081 agaaaaagca tatggcctta aaaggtatgc gataaccgga ggatgtgctt atggatcata
     1141 gtcttaattc tttgaataat ttcgatttcc tggcgcgtag ttttgccaga atgcacgcag
     1201 aaggtcgccc ggtcgatatt ctggccgtta ctggtaacat ggatgaagaa catagaacct
     1261 ggttttgcgc acgttatgcc tggtattgcc aacagatgat gcaggcaaga gagctggagt
     1321 tagagcactg atataacggg cctgatggcc cgttttagtg tttgtattag atgctagtta
     1381 taggtgattt tgaggattac gtctttacta aacggtccct caacaacagt ctctgccggg
     1441 ttcgcactaa gttctgccct gaatggtgcg gtgaccgaag gtgcactaga cgatgaaaaa
     1501 gaagcaaatt gtgagaagcg gttaaattcc gttgctgttc catctttatg aaatatcttc
     1561 aatagcagtc cattacccat atccagat
//
LOCUS       DADLSB010000341         1577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.347, whole genome shotgun sequence.
ACCESSION   DADLSB010000341 DADLSB010000000
VERSION     DADLSB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.347"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..174)
                     /locus_tag="JN331_004347"
     CDS             complement(<1..174)
                     /locus_tag="JN331_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7262260.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYP"
     gene            173..1312
                     /gene="queG"
                     /locus_tag="JN331_004348"
     CDS             173..1312
                     /gene="queG"
                     /locus_tag="JN331_004348"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY7262261.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(1295..1348)
                     /locus_tag="JN331_004349"
     CDS             complement(1295..1348)
                     /locus_tag="JN331_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262262.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT          407 a          388 c          406 g          376 t
ORIGIN      
        1 aggatacgcc gaacgacaca cctggaatgc ggcctcgcca gcgcgaagca tcagctcata
       61 gagtgtgagc cccagcacat ctgccgcctc gcgttctccg cggcggatat cgtcggcgta
      121 ccagacggtg tgtggtatac ttacggggtt tttcttcatt gtatggtccg tcatgtcaga
      181 gcccctcgat ctcaatcagt tagcgcaaaa aattaaacag tgggggctgg aactgggctt
      241 tcagcaggta ggtattaccg ataccgatct cagcgagtcc gagcccaaac tgcaagcatg
      301 gctggacaaa caataccacg gcgaaatgga ctggatggca cgtcacggta tgctgcgcgc
      361 tcgcccccat gagttattac ccggtacgct gcgtgtaatc agcgtgcgga tgaattacct
      421 tcctgctaac gccgcatttg ccagcacgct gaaaaacccc aaactcggct atgttagccg
      481 ttatgcgctg ggccgtgact atcacaaact tctgcgcaac cgactcaaaa agctgggcga
      541 gatgattcag caacattgtg tttcgctgaa ttttagaccg tttgtcgatt ctgcgcctat
      601 tctcgagcgc ccgttagctg aaaaagctgg gctcggctgg acaggtaagc actcacttat
      661 cctcaatcgc gaggccggtt cgttcttctt tttaggtgaa ttgctggtcg atattccgct
      721 gcccgtggat caaccagtcg aggaaggatg cggcaaatgc gtggcctgta tgacgatttg
      781 cccgaccggt gccatcgtcg agccatatac cgtcgatgct cgccgctgta tctcttatct
      841 caccatcgaa cttgaagggg cgatcccgga agagttgcga ccgttaatgg gaaaccgtat
      901 ttacggttgc gatgactgcc agcttatctg cccgtggaat cgctattcgc aactcactac
      961 agaagacgat ttcagcccgc gtaagccgct acacgcaccg gaactcattg agttatttgc
     1021 ctggagcgaa gagaagtttt taaaagtcac ggaaggatcg gcgattcgcc gtataggtca
     1081 cctgcgctgg ctgcgtaata tcgccgtggc attaggcaat gccccttggg atgaaacgat
     1141 tttgacagcg ctggaaagtc gtaaaggtga gcacccactt ctcgatgagc acatagcgtg
     1201 ggcgattgcg cagcaaatag agagacgaaa tgcgtgcata gtcgaagtgc aattgccgaa
     1261 aaaacagcgt ctggttcggg tgattgaaaa agggttaccg cgtgacgcct gattcatcca
     1321 cagcttgtga ataaaaataa aaacgcattg caattcaaga cgaataaaat cgtcaagtga
     1381 tcgactcgac aaaacggaat cttgattttt aacttattta tcaataggtt aaaaaatacc
     1441 attcaccgtg cgaagtttct ttgaaattaa gattacagcc tgaggctgtg gataactctg
     1501 tttacaagag tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt cgctgtggat
     1561 attttattga gagaaga
//
LOCUS       DADLSB010000342         1544 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.348, whole genome shotgun sequence.
ACCESSION   DADLSB010000342 DADLSB010000000
VERSION     DADLSB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.348"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..1544
                     /gene="yjcS"
                     /locus_tag="JN331_004350"
                     /pseudo
     CDS             1..1544
                     /gene="yjcS"
                     /locus_tag="JN331_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
BASE COUNT          394 a          422 c          407 g          321 t
ORIGIN      
        1 gcgatgctaa tggcaaacca tattatcgcg gggaagatta taaatttgat atcaatgcgc
       61 ccgcgcccga aaccgttaac cctagcctgt ggcgtcagtc gcagttaaac ggtatttccg
      121 gcctgtttaa agtcaccgac agaatgtact aggttcgcgg tcaggatatc tccaacatca
      181 cctttatcga aggtgatacc gggattattg tcatcgaccc gctggtgacg ccaccgagcg
      241 caaaagccgc ccttgacctt tacttccaaa atcgcccgca aaaacagatt gttgcggtta
      301 tttataccca cagccatacc gaccactatg gcggcgtgaa aggcattatc tctgaagccg
      361 atgttaaatc aggcaaagtt caggtgattg ccccagcagg ctttatggac gaagccatca
      421 gcgaaaacgt gctggcgggg aacatcatga gccgccgtgc gctctactct tacggtctgt
      481 tactgccgca caacgcgcaa ggcaacgtgg gtaatggcct tggcgtgacg ctggcaacgg
      541 gcgacccgag cattattgca ccgactaaaa ctatcgtcag aactggcgag aagatgatta
      601 tcgacggcct ggagtttgac ttcctgatga ccccaggtag cgaagcacca gccgaaatgc
      661 acttctatat tccggccctg aaagcgctgt gtaccgccga gaacgccacg cataccctgc
      721 acaacttcta cactctgcgc ggcgcgaaaa cccgcgacac cagcaagtgg accgagtatc
      781 tgaacgaaac gctggatatg tggggtaacg acgcggaagt cctgtttatg ccgcacacct
      841 ggccggtctg gggcaataag catatcaatg attatattgg taaatatcgc gatactatca
      901 agtacattca cgaccagacc ctgcacctgg cgaaccaggg ctacaccatg aatgaaatcg
      961 gcgacatgat taaactgccg cctgcacttg ccaataactg ggccagccgt ggctattacg
     1021 gttctgtcag ccacaacgcc cgcgcggtgt ataacttcta tcttggctat tacgacggta
     1081 acccggctaa cctgcatccg tatggtcagg tggagatggg taaacgttac gtgcaggtgc
     1141 tgggcggttc cgcacgcgtt attaacctgg cgcaagaagc gaacaagcaa ggtgattacc
     1201 gctggtcggc agaactgctg aaacaggtga ttgccgccaa cccgggtgac caggtcgcga
     1261 agaatctgca agcgaataac tttgaacagc tgggctatca ggccgagtcc gccacatggc
     1321 gcggtttcta cctgaccggc gcgaaagaac tgcgcgaagg ggtgcataag ttcagccacg
     1381 gcaccaccgg ttccccggac accattcgcg ggatgtcggt cgaaatgctg ttcgacttta
     1441 tggccgttcg cctcgatagc gcgaaagctg cgggtaaaaa tatcagcctg aacttcaata
     1501 tgagcaacgg cgataacctc aacctgacgc tgaacgatag cgtg
//
LOCUS       DADLSB010000343         1508 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.349, whole genome shotgun sequence.
ACCESSION   DADLSB010000343 DADLSB010000000
VERSION     DADLSB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.349"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1008
                     /locus_tag="JN331_004351"
     CDS             <1..1008
                     /locus_tag="JN331_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262263.1"
                     /translation="VGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAAR
                     KVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQ
                     SVEYVPLACEAHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEM
                     IKNRMAELFNQSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETL
                     EGDTNVAVVMGCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSL
                     TPEELMMLKDLFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMG
                     V"
BASE COUNT          461 a          282 c          342 g          423 t
ORIGIN      
        1 gtaggtccag atatacttgc attgcccgtc aacgtatatc atgcggattt taaaattaaa
       61 atcaataatg cagtggagaa ggcttcatca attgtgttat gtgctgtgca aaaaaatctc
      121 catgcagcaa ggaaggttct tagcgggata ataggaaatg tgccaaaaaa taataaacca
      181 aaaacaatta tttttgattt gcgttttctt agtgcaatta agcagaaagt ttttttgggc
      241 agtgaaaaac cgttgtttgt aaagcatacc gcaatgatga gggaggcatg taaggaatta
      301 ccgcagtcag tggaatatgt tcctttagcc tgtgaagcgc atagccatcg cagcgtcgcc
      361 cttgccaaaa ccgtggctgc gcccatcatg gcgacaggtg tgggcttggc attaatgtat
      421 gcaacatcat caggaccgta ttatgcgcaa agtctttctg gttcgccgga tatcgagatg
      481 ataaaaaatc gcatggctga attgtttaac cagtcggata tttctgtgcg caacaaatat
      541 agcccggctt ttgataaatg gaatgaactg gtagataaat taaatcacga aagaaataca
      601 gttccgtttg cgtgtttgac caccatttta gcgacggcga taaatgaaac tcttgagggg
      661 gatacgaatg ttgccgttgt gatggggtgt aaatctgcga aagatcgcac gatatccatt
      721 gtccttggca actcgatgtt gcaaacactc cttgaaaaac gcctcgccga tagtggagaa
      781 atagaaaatc tttttgatca acagggatat tttagttgcg attcattaac acccgaagaa
      841 ttaatgatgt taaaagacct ttttgatatt cgagttcttc atgtctctaa caaattcaat
      901 gttggattgc aaggtaatat caacactgat gttttacaag attcgttttt taaaaatgtc
      961 gatttcattc atgaatataa ctcgtatacc gtagggatgg gagtctgacg agtaaaactc
     1021 attaggatta tcagaattcg gacaatgcat tgcaaaaatg acatacaaag agagatgtgt
     1081 gcaggtactt agccgcgagg ggcatggcga agccatacag aaagtaccag tattcagaac
     1141 tccagtgaaa gcggcatagt ggccgcctgc tcaggagttt tgctatgacc cgtaatcgtg
     1201 ctgaacgtcg ccaccctatg atgcgattaa aaaaactcat cgtcgcgctg atgattgacg
     1261 tcaatgctat cggatgatgc cgggggtgtt ccggaaggaa ttccttgttt cccgaactgt
     1321 taccaacaaa attataacga tgagcaagta aagaccggtt ggtggcagta ctgaccggtc
     1381 tgtgatgaaa gattaatttc accttaaatc ctggtgctgt taggatgctg acgtttttcg
     1441 gctacaccat tcagattatg caaataaata tccctgaata taatatattc atagcaccca
     1501 taactcat
//
LOCUS       DADLSB010000344         1505 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.350, whole genome shotgun sequence.
ACCESSION   DADLSB010000344 DADLSB010000000
VERSION     DADLSB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.350"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(877..1353)
                     /locus_tag="JN331_004352"
     CDS             complement(877..1353)
                     /locus_tag="JN331_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7262264.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          353 a          398 c          376 g          378 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcagcgtaa aagatctatt ctaggcaagg gtgttgcgtt
       61 gcacgcaaca ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccatccgtt
      181 caccgttaaa acgcctcaga aatcgggaca ggatgtgtaa tcgtataacc gcgcatgcac
      241 gccgatacga ttaccacttg gtcagggctt caccccgaca ccccgcatca gtgcgtaagt
      301 gctcaatgtg gcttccggtt ctcttccgct tcctcgtcga ttcgctcgct acgctcggtc
      361 atcgacttgc ggcgagcggg atcacctctc gcatgttgtc ggtgatcccg gtgttgttct
      421 ttcggggatg agtcgccaga tagcccctca aacgcgctca aaagctctca gacgcacgaa
      481 acgggtttta gatgcccgcc agggcatgga aggcgcgaaa aacgcttaca gaggctctca
      541 ggaggtcagg cgcgccgata cccctaacca ggggctttca ggtcgccagg aaccgcgcca
      601 gccccctgca aagcccattt gcacggaaca ggcagccatg cggttggtag gtggtcgtag
      661 accgacggtg ttgcagcgat gggggtctgg ggttctcccc agacggtttc cggtagcgct
      721 caaggcgctg ccggaaaccg tggcggcgcg gttttttctg gagaacgggg agcaaaaaca
      781 cgcaaaggat tttgaggcgg caaaagagca atgcaggata gacaatcatg gtcagaacgt
      841 taacacgtag cccctgattg tccgcctgcc gctagctcaa tttgaaaaca tggccatgat
      901 tcggcgctga atatcaccgt ctttgttgac cagtgtttta gtgacaaacg ctgcatggtt
      961 tttcgtagtg ttgtatccct tccagacttc atgtggatcg agggcatacg cacaaacctt
     1021 accagcaacg gggattctaa ctaatgcatt ttttgattct aagtaattga gccaccgaat
     1081 aattgtgctt ctgctaactc caagtttttc agctaggaat tgctgatccg caacgacagc
     1141 gccacacgta ggatcgatat gctccgctaa aaatgagtac agcttcagtg ctgatatgcc
     1201 ttggctatcc ttcagcagct ctcgaacgcg ttcccaacct tttggggata cctgtgtaaa
     1261 ccttgaattt ttctttgctt cttcgagatc ctcggtgttt ttctttgcct catctgaaag
     1321 ttgtttcagt cgtttttctg aatcaataaa catgattttt tccctctgaa aatcccatgc
     1381 actaacgttg gttcaaatct aagccatcat gacacatctg tgcaacacct tgttgcgcgt
     1441 tgtcgcatcc aggatgcgac aacgttgtgt caccctggat gcgacaggta aataaaaaac
     1501 acact
//
LOCUS       DADLSB010000345         1478 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.351, whole genome shotgun sequence.
ACCESSION   DADLSB010000345 DADLSB010000000
VERSION     DADLSB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.351"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            49..1029
                     /locus_tag="JN331_004353"
     CDS             49..1029
                     /locus_tag="JN331_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY7262265.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWATALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT          406 a          345 c          345 g          382 t
ORIGIN      
        1 taaaggggcc ctgctatcaa tttgtcattt gtattacgcg aaaattatat gaacgcaata
       61 atatcgcccg attattacta tgttcttacc gttgctggtc agtctaatgc catggcgtat
      121 ggcgaaggac tgccattacc ggacagggaa gatgcgcctc attccagaat taaacaatta
      181 gcgagatttg cgcatacgca tccaggaggc ccgccatgtc actttaacga cattattcca
      241 ctgactcact gcccacacga tgttcaggat atgcagggtt atcaccatcc tctggcaacg
      301 aatcatcaaa cacagtacgg caccgttggc caggcactgc atattgcacg gaaattactg
      361 cccttcattc ctgataatgc agggattctt atcgttccgt gttgccgtgg cggatcggct
      421 tttaccgcgg gcagcgaagg gacatattca gaacggcacg gagccagcca tgatgcttgc
      481 cgttggggaa cggatactcc gctgtaccag gatttagtca gcagaacgcg agccgcactg
      541 gcaaaaaatc cgcagaacaa attcctcggc gtatgctgga tgcaaggcga atttgactta
      601 atgaccagtg actacgcgtc acaccctcaa cactttaatc atatggttga agcctttcgt
      661 agggatctaa aacaatacca ttctcagctt aataatatta ctgacgcgcc gtggttttgc
      721 ggcgatacca cctggtactg gaaagaaaat ttcccgcatt cgtatgaagc tatttatggc
      781 aattatcaaa ataatgtttt agccaatatt attttcgtcg acttccagca acaaggtgaa
      841 agaggactga cgaacgcgcc tgatgaagac ccggacgatt taagcacggg atattacggt
      901 tcagcgtacc ggtcaccgga gaactgggcg acggcactgc gaagcagtca tttcagcacg
      961 gcagcccgtc gggggattat ttctgacaga tttgtagaag caattttgca gttttggcgc
     1021 gaaaggtgag cggggagttt attcatgggg gatgtccttg ttcgttctca gagtagacaa
     1081 caaaaaacaa atgagatcaa ggtaatatct cttcacttgc gggctcgaac agcaggcaat
     1141 tgccaatata ataatctggc acaagggtga aaaacgcgca ccgcataaac cgttgttatt
     1201 gcttacagat tagccggatg ccttaataga catccgcgcc ttttcgatta tggctcggaa
     1261 atctacgaac atctacacag tttactggaa cgttttgggc cacagcgttc gcagcaccgt
     1321 cctgatatgc cgttctggcg attataagtc gacggattct ggcaaataga cgatgatgat
     1381 atgcttaatg atgttcaaag aaattaggcc attattatat tattgcttgc attttctttc
     1441 attcctaaaa gcacccgcca ttaatcccag gcgcatta
//
LOCUS       DADLSB010000346         1453 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.352, whole genome shotgun sequence.
ACCESSION   DADLSB010000346 DADLSB010000000
VERSION     DADLSB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.352"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(272..>1453)
                     /gene="ospC4"
                     /locus_tag="JN331_004354"
     CDS             complement(272..>1453)
                     /gene="ospC4"
                     /locus_tag="JN331_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HAY7262266.1"
                     /translation="PSGARSSFRSLEHLDKVSRHYISEIIQKVHPLSSDERHLLSIII
                     NSNFNFRHQSNSNLSNNILNIKSFDKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEI
                     SNHQEKLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLD
                     NFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKN
                     IDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDK
                     KMAHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDG
                     ANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFE
                     I"
BASE COUNT          453 a          258 c          242 g          500 t
ORIGIN      
        1 cccgtaaaga caggagtcaa gttcccgctc cgacataact gcgtgcaatg gattttgaag
       61 gaaccgtcag cccattcccc ggaggcaaac atgggctggc aaaagtgtag cggtattagg
      121 cacagctatt taggaaagag ggaccatctc catctcatta aatggaccac gccagacaat
      181 tcactgtcat gctgatgctg cactgtgggg acagaatcac tcatgatgac ttcgataatc
      241 gacgacatta ttatttggcc gagcttttag gtcatatctc aaatcgtttg cctaatattg
      301 ctccattttt taataaaaaa tcaatcattt ttgaatcttt gcttttcatt gcattgtcaa
      361 gcatagtgtc cccactattt gctttttgaa attttttgtt tacatcaacc aatccattat
      421 tgataaaata ttctagcact ttatagttgg caccatcttt actaagtaag tattcaacat
      481 catacagatt acaatatcta ttgttgttca ttttctccat ctctgcgaca tcttgttttg
      541 taaaattaaa tttagatagt aaataaagtg ctatatcagc ttttttattg cctaaagaat
      601 aagcaagggc ctgatgagcc atttttttat cagtcacttt attgttaatc tcttcataat
      661 tagatgcttt aatcgcatcc tccaaagata tgtctctgag ttttattttt ttgaagttat
      721 ctgtacttag cattcttggt atatgatact caagctgaaa cacaaagttt ataattctat
      781 ccagactaac aggatcaata tttttgttat aacaaaactc tctgaatcct tgatctttac
      841 tctttcttat aaaatcaata agatgcaacc caattccaag cctcatatct ttagtgttaa
      901 atattggaac atcagttttc ccctgattac catgcacata cctgctaact tcatccacaa
      961 cctctttaaa gttatctaag aaaaatgatt ggtgctcatg gtaaaaaaca ggtggaatag
     1021 cattatcaaa gtgatcggtt aatgtcatat atccatatgg agagtcatga tcaatgatat
     1081 acgcattggc gccaaaatct acagtatgat gtgttttatt tagtgggagt ttttcttgat
     1141 gattggaaat ttcaacccca aaaaaaacaa aatcgtggtt agagatttct ttaatatcct
     1201 cagagtatgt attcttatgt gtttgtatat tttcagactg aattttatca aaggatttaa
     1261 tattaagaat attatttgac agattagagt tactttgatg tctgaagtta aaattagaat
     1321 taataataat agaaagtaag tgacgttcgt ctgaagaaag aggatgaact ttttgtatta
     1381 tctcagagat atagtgcctg gacaccttat cgagatgctc aagtgaccga aatgatgacc
     1441 gtgctccaga tgg
//
LOCUS       DADLSB010000347         1421 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.353, whole genome shotgun sequence.
ACCESSION   DADLSB010000347 DADLSB010000000
VERSION     DADLSB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.353"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            62..403
                     /locus_tag="JN331_004355"
     CDS             62..403
                     /locus_tag="JN331_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7262267.1"
                     /translation="MLTTSVLNTLIHWVVFGVCIYAAHTSQALANFTGFVVAVSFSFF
                     ANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAISLVCGFV
                     YSKFIVFRDAK"
     gene            400..1329
                     /locus_tag="JN331_004356"
     CDS             400..1329
                     /locus_tag="JN331_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY7262268.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKRLPERNLFMKGILSWVGGQTDVVKYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
BASE COUNT          394 a          276 c          317 g          434 t
ORIGIN      
        1 ggttaccccc aaacgaatcg gaaaatcgat actggaaaaa ctctaactcg gtagccagtt
       61 tttgttgacc acttcagtgc tgaacacact tatacactgg gtggtttttg gtgtatgtat
      121 ctatgccgca cataccagtc aggctctggc aaacttcaca ggtttcgtag tggctgtgag
      181 ctttagcttc ttcgcgaatg caagattcac attcaaagca tcgactacag cgatgcgcta
      241 catgttatac gtgggattta tgggaatatt gagtgtgatt gttggttggg cagctgataa
      301 atgctcactt cctccaatag tcactcttat caccttctcc gccatcagtc tggtgtgcgg
      361 tttcgtctat tcaaagttca ttgtctttag ggatgcgaaa tgaaaatatc tcttgtcgtt
      421 cctgtcttca atgaagaaga agcgatacct gttttctata aaacggtacg tgaattccaa
      481 gagttgaagc catatgaagt agaaattgta ttcataaatg acggaagtaa agatgccaca
      541 gagtcaatta ttaacgcgct ggctgtttca gacccgctag ttgttccgct gtcatttaca
      601 cgcaactttg gtaaagaacc agccttattt gcagggttag accatgcaag cggcgatgct
      661 gtaattccta ttgatgtcga cctgcaagac ccaattgagg ttatccctca tcttattgaa
      721 aagtggcagg caggtgctga catggtgctt gctaaacgtt cagaccgctc aactgatgga
      781 cgactgaaac gtaagacagc tgagtggttc tataaattac acaacaaaat aagcacccca
      841 aagatcgagg aaaatgtcgg agattttcga ctcatgtctc gtgaggttgt ggagaacatt
      901 aaacggttgc ctgagcgcaa tcttttcatg aaaggcatac tgagctgggt gggtggtcag
      961 acagatgtcg ttaaatatgt acgcgcagag cgtgttgctg gcatctcaaa atttaatggc
     1021 tggaaattat ggaatctggc actagaaggt atcacaagtt tttcaacctt tcctcttcgc
     1081 gtatggactt atataggctt gtttgttgca agcatttcat ttttatatgg tgcttggatg
     1141 attatagaca cccttgtatt tggtaaccca gtacgcgggt atccctcctt gctcgtatca
     1201 atacttttct tgggtggcgt gcaactgatc gggattggtg ttctcggaga atatataggc
     1261 agaatatata ttgaagtaaa aaatagacct aaatacatca taaaaaaatc tcatcgaggt
     1321 aacccatgat taaaattaat ttatttaaaa attcaaatct ccttgccttc atatcatgtt
     1381 ttgcaatatc aatttattgt tattgggggt ggttgtacga t
//
LOCUS       DADLSB010000348         1418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.354, whole genome shotgun sequence.
ACCESSION   DADLSB010000348 DADLSB010000000
VERSION     DADLSB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.354"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..183)
                     /gene="fimA"
                     /locus_tag="JN331_004357"
     CDS             complement(<1..183)
                     /gene="fimA"
                     /locus_tag="JN331_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY7262269.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVR"
     gene            complement(664..1260)
                     /gene="fimE"
                     /locus_tag="JN331_004358"
     CDS             complement(664..1260)
                     /gene="fimE"
                     /locus_tag="JN331_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY7262270.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          425 a          305 c          273 g          415 t
ORIGIN      
        1 acgaacctgt cccaactgaa cagtttgatc aacagagcct gcatcaactg cgcaagcggc
       61 gttaacaact tcccctttaa agtgaatggt cccaccattt accgtcgtag tatcggccag
      121 agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag ttttaatttt
      181 catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg ttctgtacac
      241 tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa aagatgaaac
      301 atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt cagtttattt
      361 gttggcttaa tattctatta ttatctttat ttatagattt ttatattgca tgaggtggtt
      421 tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt tacatatagc
      481 ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata gttagatgct
      541 ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata cgtaacttat ttatgatatg
      601 gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta tcaataagtt
      661 aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt cccataatcc
      721 ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat ttcgatgccc
      781 gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat aaccgcaagc
      841 atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac cggcatcgcg
      901 aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa taaatatagc
      961 gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt ccacggcttc
     1021 acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc ggcgaatatt
     1081 tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt cactaatacg
     1141 catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg ttgccccgta
     1201 acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac gtttactcac
     1261 aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa cctcaatcgg
     1321 tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg ctatcagtca
     1381 attacattaa acatcataca aaaataaaat atcactga
//
LOCUS       DADLSB010000349         1399 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.355, whole genome shotgun sequence.
ACCESSION   DADLSB010000349 DADLSB010000000
VERSION     DADLSB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.355"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            47..721
                     /locus_tag="JN331_004359"
     CDS             47..721
                     /locus_tag="JN331_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7262271.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYRRE
                     FKVRLAKQALQPGAVVARIAREHDINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIRE
                     MKGGIR"
     gene            718..1065
                     /gene="tnpB"
                     /locus_tag="JN331_004360"
     CDS             718..1065
                     /gene="tnpB"
                     /locus_tag="JN331_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7262272.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            1085..>1399
                     /locus_tag="JN331_004361"
     CDS             1085..>1399
                     /locus_tag="JN331_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7262273.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSE"
BASE COUNT          372 a          361 c          366 g          300 t
ORIGIN      
        1 cctgtctggc aacaatcgcg cccatctata ttgatggaca cgaacgatga attcccagac
       61 aacaaaagat attccctgct tccgttctta tttgcctgat gccctgcgtt taagatttga
      121 agataaactg accatccggg ccatcgctca gcgtctgggt ctcagtcatt ccacaataca
      181 tacgcttttt cagcgatttc ttgcatccgg tatcgcatgg ccattgcccg attcagtttc
      241 attcgctcaa cttgacgcca tcctttatgc caacagaaag aaggaattaa cagagcctca
      301 aatcagagaa ggctcatggc gaaaagaacg gcgaaccagc tatcgccgtg aatttaaggt
      361 ccgtctggct aagcaggcgt tacagcctgg ggctgttgtt gcccggatcg ccagagaaca
      421 cgatatcaat gataacctgc tgtttaaatg gaaaagccag tacgaggacg gcttactgag
      481 cgatgatgac atacaggaat gcatgcctgt cccggtggca ctgactgata cgccggagcc
      541 gaccagacca gttacaaatc ccttctggcg taacaagcat gatgagcgcc ctgagggggc
      601 tcccggaaac gtcccacggt gcgagctgca tcttaaatca ggtgtggtaa aactgtttga
      661 ccctctcact ccggaactgt tacgggcgct aatccgcgaa atgaaagggg gtatccgatg
      721 ataacgctgc cgaccggtac caaaatctgg atcatcgctg gcatcacaga tatgcgttgt
      781 ggcttcaatg gcctggcttc gaaggtgcag aacacgctga aagatgaccc gttctccggg
      841 catatcttcg tcttccgggg ccgcagtggc aaaatggtga aaatactgtg ggccgatcgt
      901 gacgggttat gcctgttcgc caaacgcctg gaacggggcc gcttcgtctg gccggtgacc
      961 cgggaaggga aagtgcacct gacgccagct cagttatcca tgctactgga ggggatcgcg
     1021 tggcaacatc ccaaacggac agaacggcct ggcatccgga tataacccgt gataaaacaa
     1081 gggaatgaac aatgaactcc ccgatgatat tgagctgctt aaagccatgt tgcgtaagca
     1141 acagagtcgg cttcgacagt atgcctgtca ggtcgcgggc tatgagcagg aaattgaacg
     1201 gctgaaagcg caactcgaca ggttgcgtcg tatgttgttc ggccagagtt cagagaaaaa
     1261 gcgtcataag cttgaaaatc agatccgaca ggcagaaaaa cgactgtcgg aactggaaaa
     1321 ccggctgaac acagccagaa atcttctgga agatgcatcg tcagtcacag attcacctga
     1381 caccagtccc ccgtcagaa
//
LOCUS       DADLSB010000350         1398 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.356, whole genome shotgun sequence.
ACCESSION   DADLSB010000350 DADLSB010000000
VERSION     DADLSB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.356"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            43..630
                     /locus_tag="JN331_004362"
     CDS             43..630
                     /locus_tag="JN331_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262274.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(692..>1398)
                     /locus_tag="JN331_004363"
     CDS             complement(692..>1398)
                     /locus_tag="JN331_004363"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7262275.1"
                     /translation="EDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMYEYLTTL
                     VKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLTIDNYRE
                     HDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGG
                     DFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPS
                     LMVIADKAAAAELAFG"
BASE COUNT          359 a          324 c          331 g          384 t
ORIGIN      
        1 tactttatct ttgggctact caaaagcaga caggatgtta ctatgactca aaatatcagg
       61 ccgttacccc aattcaaata tcatcccaag ccactggaaa caggcgcatt tgaacaggat
      121 aaaaccgtag agtgcgattg ctgtgaacaa cagacgtcag tttattactc gggtcccttt
      181 tattgcgttg atgaagttga acatctctgt ccgttgtgta ttgcggacgg ttctgctgct
      241 gaaaagtttg caggtagttt tcaggatgat gccagcatag aaggtgttga atttgagtat
      301 gatgaagagg acgaatttgc cggtattaag aacacatatc ctgatgaaat gctgaaagag
      361 ttggttgaac gcacgccagg ttatcatgga tggcaacagg aattctggct tgcgcattgt
      421 ggcgatttct gtgcttttat cggctatgtg ggctggaatg atataaaaga tcgcctcgat
      481 gaatttgcca accttgaaga agattgtgag aatttcggta ttagaaattc tgatctagct
      541 aaatgcctgc aaaagggtgg tgattgtcag ggttatctct tccgctgtct ccactgcggc
      601 aagctgagac tgtggggtga tttttcgtag ttatttaaat aatgagaaca ggccggagcg
      661 taattcatac atccggcctt atttcttcag cttaaccgaa cgctaattct gcggcagcgg
      721 ctttatcagc gattaccatc agcgaaggat gcagctgcaa cacggaagct ggaacatctt
      781 cggtaaccgg accttgcagc acatttttca gtgcctgcgc tttacctgcg ccactgacaa
      841 ttatcagtaa gtttttcgcc gccatgatgc ttttcggccc catcgtcacg tagctgtcag
      901 gcactaacga aaagtcgccg cccaactcac catgcgccac aatatcaacc atctctccct
      961 ggatcgggaa ctccacggtt tgctcatgga aatgggtcgt attcggcaaa ttgccacaaa
     1021 aatgaccatc tgcacctaat cccagcacca ccaaatccag gccgccttca cgcgccagtt
     1081 tctgatcatg ctcgcggtag ttatcaatag tgagcttctg gatattctct tctttgatcc
     1141 ccgcaggggt gaaaaacaga ttacgcagat tggtaatcgt tacgccttct ccctctttgc
     1201 cgcgaaaggg aatttcatcg aaattataga aatagcagtt atcgtaccaa ggcttacctt
     1261 taaccagggt ggtgaggtat tcatacatgc ctttgggcgt gctaccggcg gtaattgcca
     1321 ggttaacacg acgcgtcttc gacatataac caagcagatg gtgtgccgcg acacggctca
     1381 tttcctggta atcttcgg
//
LOCUS       DADLSB010000351         1396 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.357, whole genome shotgun sequence.
ACCESSION   DADLSB010000351 DADLSB010000000
VERSION     DADLSB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.357"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..780)
                     /locus_tag="JN331_004364"
     CDS             complement(<1..780)
                     /locus_tag="JN331_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7262276.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQ"
BASE COUNT          374 a          222 c          338 g          462 t
ORIGIN      
        1 ttgcagagca tgggatgaca atgggttatc actaatatct attgaacatt cattcctcag
       61 attaagaatg ctttccggga tatgacttat ctgatttatg tcaaaataac cgttccttaa
      121 attttccggc aggtatggaa tatcggttaa catattgtcg attgcccata tggagtgaat
      181 gttgtgaggt agagggggga gttcactaag aagattccct attgcgctaa tctcttgtaa
      241 agaaaatggt aaaggtggta aaactgccag cccattacat gatacatcta atgtttccag
      301 tagttctggt aaaacaggaa gagaacataa ttgattattt gagacatgaa gctccttcaa
      361 agatatgggg agtgtgggta gtgtgattag ttgattgtgg gacgcattca ataatttaag
      421 tccttgaggc aaagcaggca gttcaataag tctgttatag ctgacattaa gctgtgtaag
      481 ggacgcaggc aatggggaga ttaagcttaa attattttta cttatattaa ttgatttaat
      541 tcccgggggg atttcaggta atgttgtcag gcctaactca gacaagtcca ggttcgtctc
      601 ttggttttgt agacatgata ctagtcgctg aaaagcgatg tctcgttgtt cttcttgtat
      661 gcggttattt ttccattcag tccaatgggt tagataattt tcatatgcgc ggctagtgtc
      721 gattgaataa gtggagaacg aatttgaaat taatctgtga ttgttatttg tcgggagcat
      781 aaatatcagg gcgtattctt tatgttgaga ggtgttggat tcttttttat ctgctttgca
      841 atgttattgg ttcccttcag cagtagacag gaacttctgg aggaggcgga tatatagttt
      901 gtgtaagcat attaactcca tatgttatat attgaagaac ttctgcttta cactatccta
      961 aactggatcg gtttaatgta aaaaaacgat actttttggg ggagggggca atttttcctt
     1021 aaggtatgaa aaaaggagct gaagctatat aatagctatt aatgccactg gtatcagtga
     1081 atcatgtata taaaaaactg tgttaaggag gctgtccatt agcggttttt tattcttctg
     1141 gaaccgcggc atctttgcgt atctttttgt cttctcaccg tctggtcggt gttttgctga
     1201 gactgctaat ttcctgtctt tgctagtgct gtactggtac tgcccaatca tgattggcgg
     1261 gggcgggcgg agttgagacc gcagacacgt cgtatgcaag aacgtgctgc ggctggctgg
     1321 tgaacttccg atagtgcgag tattgaatga tttccagccg ttactgattt tacgtgctaa
     1381 ttagtgaaca aaccac
//
LOCUS       DADLSB010000352         1381 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.358, whole genome shotgun sequence.
ACCESSION   DADLSB010000352 DADLSB010000000
VERSION     DADLSB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.358"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..211
                     /locus_tag="JN331_004365"
     CDS             <1..211
                     /locus_tag="JN331_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HAY7262277.1"
                     /translation="GHTVLNPAVLPDGLTQPQYMDICMAMIRCVDAIYMLKGWQRSAG
                     AKAELALAEKLGHAVIFQETASVQD"
     gene            198..653
                     /locus_tag="JN331_004366"
     CDS             198..653
                     /locus_tag="JN331_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7262278.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSDPDVLALLKELEVKDKRIAELTDVLTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
     gene            751..1017
                     /locus_tag="JN331_004367"
     CDS             751..1017
                     /locus_tag="JN331_004367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262279.1"
                     /translation="MTELTKEQLIEEAKLKIAIAKCHPNSGVARVEGELFKIALASLE
                     AEPVAWKAIFTQIDHEYNTFTALYSDKAEAERWVRLHKVGDFRA"
     gene            1140..1271
                     /locus_tag="JN331_004368"
                     /pseudo
     CDS             1140..1271
                     /locus_tag="JN331_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
BASE COUNT          400 a          275 c          376 g          330 t
ORIGIN      
        1 agggcatacc gtcttaaacc cggcagtact tccggacggg ctgacacagc cgcagtacat
       61 ggatatctgc atggcgatga ttcgttgtgt ggatgcgatt tacatgctga aaggctggca
      121 gcggtcagca ggcgctaagg cagaactggc gctggcggag aaactggggc atgcggtgat
      181 ttttcaggag acagccagtg tgcaagacta attaccaggc attacgtgaa cgttattcac
      241 caattcaggt gccggaatgt cctgtttgtg gcgatgaaat gtcgatacag cgcatatttt
      301 ccagaacgca tattgtgtat gcctgcacag gtgaggggga tgatggatat tttaaaactg
      361 gtcgaacttt tgcggatgag cattacctga aatcgcgcgt aaccgtcgtt gatgttagcg
      421 atcctgacgt actggcttta ctgaaagagc tggaagttaa agacaaacgc attgcagaat
      481 tgacagatgt gcttacacaa atgattaatg cgcacaaaac cacaattcgt tttggtcatg
      541 aacgcataac tgaatgtggt ggtgattgcg actcgccgga aaagatgatt tcagaaaatc
      601 cggatatcag aatggcagag gctgttttga gagcaggaat aaaaactgaa taattaaatt
      661 tagcactgta aataaaattt aatccttaac cggagggatt tctgcaccct cagaacatca
      721 ggaggccgcc cgaaatggcg gtaatgaaaa atgactgaat taacaaaaga gcaattaatc
      781 gaagaagcta aattaaaaat agcgattgcg aaatgccatc ccaattcagg cgtggcacga
      841 gtagagggtg agttattcaa aattgcactg gcatcgctgg aagctgagcc ggtggcgtgg
      901 aaggcaatct tcacgcaaat tgaccatgaa tataatacgt tcaccgctct gtattctgac
      961 aaagcagaag cagaacggtg ggtgcgactg cataaagtag gtgattttcg ggcataacac
     1021 cactttatgc agcatcgcca gcgctggtag tgcctgataa gttgccgcgt gaatacataa
     1081 gaggttggcc tcttgcgtat agtgattatg ctgaaggatg gaacgattgt cgtgcagcca
     1141 tgcttcaggg atggcagcca tgccatcaaa ttaccgcctc aatggcaaaa gccattgcgc
     1201 ttaaacttgg tgctgaactg aataacgaag aagcagagat tttcgcggat gggtataacg
     1261 ccgccatgct tcatgccgga aactgaaccg tagcaggagg tgaatcgatg agctggcctg
     1321 aagcattcac aacggtagga attgcaatgg cggtggcgct ggttgtgtat tcgatttgcc
     1381 g
//
LOCUS       DADLSB010000353         1376 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.359, whole genome shotgun sequence.
ACCESSION   DADLSB010000353 DADLSB010000000
VERSION     DADLSB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.359"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            7..807
                     /locus_tag="JN331_004369"
     CDS             7..807
                     /locus_tag="JN331_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY7262280.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIAHDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTVRTTGRAEATIL
                     VDIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            1004..>1376
                     /locus_tag="JN331_004370"
     CDS             1004..>1376
                     /locus_tag="JN331_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7262281.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEG"
BASE COUNT          340 a          342 c          346 g          348 t
ORIGIN      
        1 ggaaccatga acattacccc gtttccgacg ctttcgacgg caactataga tgccataaat
       61 gttatcggac agtggctggc gcaggatgat ttctccggtg aggtgccgta tcaggctgat
      121 tgcgtcatac ttgcaggaaa tgcggttatg ccgactatcg atgcggcatg taagattgct
      181 cacgatcagc aaattccttt actgattagt ggtggtatcg gtcactcgac cacttttttg
      241 tatagcgcca tcgcacagca tccgcactac aacactgtcc gcaccactgg cagagcagaa
      301 gcgaccatcc tggtggatat cgctcatcag ttctggcata ttccgcatga aaaaatctgg
      361 attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct ttagcatcgc gctattgaat
      421 caggccgtag aacgagttca tacggctatc gttgtacagg accccaccat gcagcggcgc
      481 acgatggcaa cgttccgccg tatgaccggg gacaatcccg atgcaccacg ctggttaagt
      541 tatcccggat tcgttcctca gttaggaaat aacgcagaca gtgtaatctt tattaatcag
      601 ttacaaggat tatggccggt tgagcgttat ctctcactac tcactggcga gctgccgcgt
      661 ttacacgatg atagcgatgg ctacggtccc cgcgggcgag attttatcgt tcacgttgat
      721 tttccggcag aagtcatcca tgcatggcaa acgctgaaac atgatgcggt gctcatcgag
      781 gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt gtgaaccact tgtttgcaaa
      841 cgggcatgac tcctgacttt tatttctgcc ttttattcct tttacacttg tttttatgaa
      901 gccctccaca gaattgtcct ttcccgatgc cgtctctctg atgattgatg ttaattaaca
      961 atatattcac cgaaaacaaa catataaatc acaggagtcg cccatgtcag tacccgttca
     1021 acatcctatg tatatcgatg gacagtttgt tacctggcgt ggagacgcat ggattgatgt
     1081 ggtaaaccct gctacagagg ctgtcatttc ccgcataccc gatggtcagg ccgaggatgc
     1141 ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa tgggaagcgt tgcctgctat
     1201 tgaacgcgcc agttggttgc gcaaaatctc cgccgggatc cgcgaacgcg ccagtgaaat
     1261 cagtgcgctg attgttgaag aagggggcaa gatccagcag ctggctgaag tcgaagtggc
     1321 ttttactgcc gactatatcg attacatggc ggagtgggca cggcgttacg agggcg
//
LOCUS       DADLSB010000354         1358 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.360, whole genome shotgun sequence.
ACCESSION   DADLSB010000354 DADLSB010000000
VERSION     DADLSB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.360"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            210..998
                     /gene="virF"
                     /locus_tag="JN331_004371"
     CDS             210..998
                     /gene="virF"
                     /locus_tag="JN331_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY7262282.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(940..1261)
                     /locus_tag="JN331_004372"
                     /pseudo
     CDS             complement(940..1261)
                     /locus_tag="JN331_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          443 a          230 c          247 g          438 t
ORIGIN      
        1 gtccctttca gtgcaaatac ttagcttgtt gcacagagaa atagaagctg cataagctct
       61 ttcttcaaaa aatgtaaata aagttaaata taggaaaaat tacttaatct atcttaataa
      121 cggaaagatt ttgtatacaa tcactgttac agaaatttct tagttactct gtaaacacta
      181 aatatagttt ggtatattct gttgaattta tgatggatat gggacataaa aacaaaatag
      241 atataaaggt tcgcttgcat aactatatta ttttatatgc aaaaaggtgt tcaatgacgg
      301 ttagctcagg caatgaaact ttgactatcg atgaagggca aattgctttt atagagcgaa
      361 atatacaaat aaacgtctcc ataaaaaaat ctgatagcat taatccattt gagattataa
      421 gccttgacag aaatttatta ttaagcatta ttagaataat ggaaccaatt tattcatttc
      481 aacactccta ttctgaggag aaaagggggt taaacaaaaa aatattcctc ctctctgagg
      541 aggaggtttc tatcgatttg ttcaaatcta taaaagagat gcctttcggc aaaagaaaga
      601 tctatagttt agcttgcctt ttatcagctg tttctgatga ggaagcttta tatacttcga
      661 tatcgatagc ttcttctctt agtttttctg atcagataag gaagattgtt gaaaaaaaca
      721 tcgagaagag atggcgtctt tctgatattt caaataactt gaatttatca gaaatagctg
      781 ttagaaaacg attggagagt gaaaaattaa catttcaaca aatccttctt gatattcgca
      841 tgcatcatgc agcaaagctt ttattgaata gtcaaagcta tattaatgat gtatcaagac
      901 ttatcggaat atcaagccca tcttatttta taaggaaatt taatgaatat tatggtataa
      961 ctccaaagaa attttactta tatcataaaa aattttaaat gcttcatagc ccatcgctat
     1021 tgccagatgg gttttcccca cacctgatgg ccccagtaat acgatatttt cattacgttc
     1081 tatgaagctg agtgagcgta acgactggag ttgcttctgc ggtgctccgg tggcgaatgt
     1141 gaagtcatac tcttcgaacg ttttcaccgc cgggaaggct gccattcggg tatacatcgc
     1201 ctgtttacgt tgatgacgtg ccagtttttc ttcatgaagc atgatgctcc aggaagtcca
     1261 tataactcca ttcctggtct actgcctgtt gtgacagcgc aggcgctgcg cttatgtact
     1321 gcacccattt tgttggacga tgaaatggaa tagcccct
//
LOCUS       DADLSB010000355         1335 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.361, whole genome shotgun sequence.
ACCESSION   DADLSB010000355 DADLSB010000000
VERSION     DADLSB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.361"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..378
                     /locus_tag="JN331_004373"
     CDS             <1..378
                     /locus_tag="JN331_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262283.1"
                     /translation="FSGNVVQQMLDVASAADINIDLGNVEKVTIWPKGQNRNIPPVLI
                     SPDHGLTGYPVYTMTGLSATTIFCPDLFTGRPAHLEPSLPDMTGDYTITGVIHTITSR
                     TVGGPWSSNCTMMRAEENGTTTQ"
     gene            359..1036
                     /locus_tag="JN331_004374"
     CDS             359..1036
                     /locus_tag="JN331_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7262284.1"
                     /translation="MAQPLSNPTDVNSEINAQDFMLRQFLGRHAFITLGRVVAVEEGF
                     IEARPMVMGVAADGSPVEHEVIYNIPVWRLQGGGNAVIMPPHVGDIGFLAICDRDISA
                     VKATRQAAMPGSKRTHNYADAIWLGGVLNGDPVQFVVFDDNQIRIVSPWKVEISAPEG
                     VINAPKSFTVNSPQIALNGDTAVSKGLDVTGQSRLSGGSNIGGIDFGNHVHGGVESGG
                     STTQGPR"
     gene            1049..>1335
                     /locus_tag="JN331_004375"
     CDS             1049..>1335
                     /locus_tag="JN331_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262285.1"
                     /translation="MQSRSLLLDTGTWDILLDDTGNLAITDNPHAVAQDVACACSTFL
                     GECWYDSTAGIPYWPRILGHWPGTQLVNATLQQEALKLPTVSAAVCQVTAD"
BASE COUNT          309 a          341 c          381 g          304 t
ORIGIN      
        1 ttttccggta atgttgtaca acaaatgctg gatgtcgctt cagccgccga tattaacatt
       61 gatctgggga atgtggagaa agtcaccatc tggccgaaag ggcagaaccg gaatattccg
      121 ccggtactga tttcgccgga tcacggactg actggctatc cggtttacac catgaccgga
      181 ctcagcgcca ccacgatatt ctgccccgat ctctttactg gcaggccagc gcatctggaa
      241 ccgtcactgc ctgatatgac gggcgattat acgatcacag gggtgataca caccattact
      301 tcgcgaaccg tgggcggtcc gtggagttcc aactgtacca tgatgagggc tgaagaaaat
      361 ggcacaacca ctcagtaacc cgacagacgt gaacagcgaa atcaatgcac aggactttat
      421 gctgcggcag tttctcggac gtcatgcgtt tatcactctg ggacgggtgg tagccgtgga
      481 agagggattt attgaggccc gaccgatggt aatgggcgtt gcagcagacg gttccccggt
      541 tgaacatgag gtgatttata acattcccgt atggcgacta caggggggcg gtaatgcggt
      601 gattatgccg ccacatgtgg gggatatcgg ttttcttgcc atctgcgatc gggatatcag
      661 tgcggtaaaa gccacgcgtc aggctgcgat gccgggatca aagcgtaccc ataattatgc
      721 tgatgccatc tggctgggtg gcgtgctcaa cggtgatccc gtccagtttg tggtatttga
      781 tgacaaccag atacggattg tatctccctg gaaagtggag atatccgcgc ctgagggtgt
      841 gattaatgcg ccgaaaagct tcaccgttaa ttcaccacaa attgcgctga acggcgatac
      901 cgccgtcagt aaggggcttg acgttaccgg gcagtccagg ctttccggcg gttcgaacat
      961 cgggggtatt gattttggaa accacgttca cggcggcgtt gagtccggcg gctcaaccac
     1021 gcagggaccc cggtaaacag gagaaaacat gcagtcacga tcacttcttc ttgacaccgg
     1081 gacatgggat atcctgcttg atgataccgg aaatcttgcc attactgata atccccatgc
     1141 ggtagctcag gatgtggcgt gtgcgtgcag tacttttctg ggggagtgct ggtacgactc
     1201 aacggctggc ataccttact ggccacgcat cctcggacac tggcccggca cacaactggt
     1261 gaatgccact ctgcaacagg aagcacttaa actgccgacc gtgagcgccg ctgtctgcca
     1321 ggtcacagct gatca
//
LOCUS       DADLSB010000356         1301 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.362, whole genome shotgun sequence.
ACCESSION   DADLSB010000356 DADLSB010000000
VERSION     DADLSB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.362"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(172..>1301)
                     /locus_tag="JN331_004376"
     CDS             complement(172..>1301)
                     /locus_tag="JN331_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7262286.1"
                     /translation="KQPEGAVRPLVIESVTKIMACGTTLMGYTQWCCSSPDCCHTKKV
                     CFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTLPCQYWSLVFHNRWLLAEM
                     SRIAADVILEICHQTDVEPGIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHF
                     YARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKREWNRFLDTHYRRGWNVNVSR
                     VMDNATHVAVYFGSYLKKLPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMSGDEFM
                     ERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMYQRLLK
                     VDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT          296 a          402 c          294 g          309 t
ORIGIN      
        1 ccccaaaaag attaaaaaaa caccacaaaa cggatgtttc ttcaacacca cttttgctcc
       61 atatgaacgg aaccgacgat taaactggat ggctctgatt gattcagggt atgaatggcg
      121 gttttttgct ccgtttccct caaaatggac gcaacttccc ctctgcggct ctcagccgca
      181 ccaccgcatc cgggccagcg gctcatgcat caggacctgc tctgccagac ggtagccccg
      241 cttcagcccc gtaaaacgca tctgactccc gcacagcacg cacttcagcg ggtcaacctt
      301 cagtaacctc tgatacatcc ctctccaggt gatttgcatc gccgtttttc tcactgtctc
      361 cgttatgatg tacaccactt cttccagtaa ccgccgtttc gccggactca aaaaaccgta
      421 gtacctcacc atacggaacc ccttatccgc cacatgccag gagaaccttt ccatgaactc
      481 atctccactc atcaacaggt attcttcccg ttttgttcgg tgactgttgt aacgcagacc
      541 gatttcatcc tgaccggcat aatgctccag acgactcatc ggcaccggta gctttttcag
      601 gtaagagcca aagtacaccg ccacatgggt ggcattatcc atcacccggg atacgttgac
      661 attccagcca cggcggtaat gcgtgtccag gaagcgattc cattcccgtt tactgcttcc
      721 ttctgctgcc agcgcatccg gcatcaccag gtcagggtat ttccgtgaca gcaaccgtgt
      781 tatccggtag cgccacatgc tcatcacctt acgggcgtaa aaatgaagat ttttccaggt
      841 gtggcccgac gtcacaccac cggcagttgt cgataaatgg atatgcggat gccactgctg
      901 gtcacgcccc catgtgtgga tcaccgtgaa tatccccggc tccacatctg tctgatggca
      961 gatttccagt atcacatccg ctgcaatgcg gctcatctct gccagtaacc accggttgtg
     1021 gaacaccagg gaccagtact ggcagggaag tgtgaacaca atatgctgcc acgggcagtc
     1081 ggggaccagg ctcagcagat actgtatcca ctgtgcgcca gccttcaccc cgcagtgcgg
     1141 gcaggagcgg cttttacacc ggaagcagac cttttttgta tggcaacagt ccggtgatga
     1201 acaacaccac tgcgtgtagc ccatcagtgt ggtcccgcag gccatgattt ttgtcactga
     1261 ttcaattacc aacggacgga ctgccccttc cggttgtttc t
//
LOCUS       DADLSB010000357         1281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.363, whole genome shotgun sequence.
ACCESSION   DADLSB010000357 DADLSB010000000
VERSION     DADLSB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.363"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1019
                     /locus_tag="JN331_004377"
     CDS             <1..1019
                     /locus_tag="JN331_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY7262287.1"
                     /translation="FSCTINYVQSLRELYTDVIEEFYWVALPLTTQNSLSQYQPEWQC
                     WEPDVEWVRQPPQDAITAPDFFCFYQPGMTFEQFVREFAEWFSQKRPAAMMIGIRADE
                     SYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTWYANHQNLCNP
                     LYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVSGVKSGGIYAG
                     HDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHWYQKKGIEVPQ
                     TQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERIKGKRQEWGIL
                     CNND"
     gene            992..>1281
                     /locus_tag="JN331_004378"
     CDS             992..>1281
                     /locus_tag="JN331_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY7262288.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK"
BASE COUNT          371 a          313 c          291 g          306 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct ggtatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaagccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag cccctttcgt gaagagccag tggattgcgt
     1141 tctgtgggtt aaaaacagcc aacttatgcc taatgattac aacccaaaca acgtggcacc
     1201 acccgaaaaa aaactgctgc aaaaatccat tgaaatagat ggttttaccc aacccattgt
     1261 ggtcacacat acggataaaa a
//
LOCUS       DADLSB010000358         1268 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.364, whole genome shotgun sequence.
ACCESSION   DADLSB010000358 DADLSB010000000
VERSION     DADLSB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.364"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..1246)
                     /locus_tag="JN331_004379"
     CDS             complement(<1..1246)
                     /locus_tag="JN331_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HAY7262289.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQL"
BASE COUNT          265 a          356 c          328 g          319 t
ORIGIN      
        1 caagttgtcc catacttgca agatcgcgca tcagctccgg tatacgtccc ggtgaagcgc
       61 cctgcaatgt catcagcatt ctcatcacca ttccgcatga ttctgagaaa ctcagttgat
      121 tcggccagta acctttcaga tgttccgcca ttttaatcat ctgatatctc accagattat
      181 aagccagtaa gacaccccac agctcttgct ccacaagctc cggcttttta cttctcagcg
      241 tcagcctgct cagttgcatc gtctgtttta tctccctgta tcccagttcg atttcccagc
      301 gatgactgta cagatccgcc atttctcctc cggggaagcg catggcgtcc gtcatcgacg
      361 tcagcagatg gcagactttt cctttgcgcg tcacggtcag caggcgggct gtcacttcat
      421 ttcccagtcc cggccacttt tttcgtgcct gcgggctggt tttcagcttc accagatgat
      481 cgcctttacc cagttttctg agctcttcat attgcgctcc ctttctgaga ggtatcatcc
      541 agtggcggtg ttctcccgcc aggctccagg catttaacag tcccagtgag taataacctt
      601 tatccattaa cgtcagagtg ttatcgccgg tttgttctat aagttgctca gcaagctcat
      661 tttcgctgtt cttcatcgtg ccgaaggctg cagccgtcag cagatggctg gtcagttcca
      721 tctggcagac cattttgacc tgcgggtaga gcgccgggtt cccggcatgt gtctggcggg
      781 ggaaggctgc atcgttctct ggtgtatccg gtgtgcgcca gaacacacca tcgatggcca
      841 gcagggtcag gccgcaccag tgcggatgcg gcgtggcgtt atgccagagc tgcgctgttt
      901 tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga ataacggcac
      961 tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc acaatctggt
     1021 gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc atttcgaggg
     1081 gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga gagatgagtt
     1141 cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg taacgggata
     1201 ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga gcgtttccgg
     1261 attcttac
//
LOCUS       DADLSB010000359         1264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.365, whole genome shotgun sequence.
ACCESSION   DADLSB010000359 DADLSB010000000
VERSION     DADLSB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.365"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..84
                     /locus_tag="JN331_004380"
     CDS             <1..84
                     /locus_tag="JN331_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7262290.1"
                     /translation="RDELIDTTQARPIQYCINGTWYNAGSI"
     gene            87..620
                     /locus_tag="JN331_004381"
     CDS             87..620
                     /locus_tag="JN331_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7262291.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQMENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(649..1176)
                     /locus_tag="JN331_004382"
     CDS             complement(649..1176)
                     /locus_tag="JN331_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7262292.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEERARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1178..>1264)
                     /locus_tag="JN331_004383"
     CDS             complement(1178..>1264)
                     /locus_tag="JN331_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7262293.1"
                     /translation="NSADNIAGVYYRPIQKYINGTWYNVASV"
BASE COUNT          360 a          282 c          277 g          345 t
ORIGIN      
        1 agagatgaac taattgatac aactcaggca aggccaattc agtattgcat taatgggacg
       61 tggtataacg cggggagtat ttaacgatga tgcacttaaa aaacatttct gctggtaatc
      121 ctgaaacaaa agagcaatac cagctaacga aacaatttaa catcaaatgg ctttatacag
      181 aggatgggaa aaactggtat gaggaacaaa agaactttca gcctgatacg ttgaaaatgg
      241 tctatgacca caacggcgtt attatttgta ttgaaaagga tgtttcagca attaatccgg
      301 aaggcgcaag cgtcgttgag gttcctgata ttacagcaaa tcgccgggct gatatttcag
      361 gtaaatggat gttcaaagat ggcatagtga taaagcgaac ttataccggg gaagagcaga
      421 ggcaacaaat ggaaaatgaa aagcaaagcc tgttgcaact tgtcagggat aaaacccagc
      481 tatgggactc acagctacgg ctgggcatca tttccgccgg gaataagcag aaattaaccg
      541 agtggatgct ctacgcgcag aaggtcgaat ccacagacac ctccagtctg ccagtaacgt
      601 ttcccgaaca accagaatga gagaaggccc gctatcgggc cttaattttt actctggttt
      661 ttgtggccat tctggctttg ccgtatccac acggctgacc agaacactgt agcgttccca
      721 tgactccagt cgtgcgcgtt cctcatctgt tgccatattc agcctgacag agcgctccag
      781 cggctggatg actgattcag cttcggaaag caatgcggct ttttgtgatt cggcctgttg
      841 ctgctgttcg tctgccgtat aaatccgctt aaccacagct ccgtccttaa acatccactt
      901 cccggaatca tcggcacgac gatttgctgt tatatcgggg acctcaacga cgctaaagcc
      961 ttcaggatta agcgtggagg catctttagt gatggcgaca ataatattat tttcatcgta
     1021 aacaatcttt attgtatctg gctgaaagtt cttcacttcc ccataccagt tttttccgtc
     1081 ttctgtccac aaccagatta cgtcaaaatt ctttgttaat tcgtactgtt ccagtgtttt
     1141 agcgttaccc gctttaatgt tctttaagtg catcatatta aacgctcgct acattatacc
     1201 aggtgccatt tatatacttt tgaatcggtc tgtaataaac acccgcaata ttatcggcag
     1261 agtt
//
LOCUS       DADLSB010000360         1251 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.366, whole genome shotgun sequence.
ACCESSION   DADLSB010000360 DADLSB010000000
VERSION     DADLSB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.366"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..1240
                     /locus_tag="JN331_004384"
     CDS             <1..1240
                     /locus_tag="JN331_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HAY7262294.1"
                     /translation="KPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINT
                     AFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQS
                     EIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNG
                     KTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYN
                     VLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEER
                     LAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEID
                     NNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKL
                     NDWPSNQVHELLPWNFSSVK"
BASE COUNT          337 a          300 c          363 g          251 t
ORIGIN      
        1 aaaaccgctg ccggcagaac ttccccggga gacacatcgc cttctgcctg ctgaaaccag
       61 ttgcccggcc tgtggaggtg ttctgaaaga aatgggggaa acaatctcag agcaactgga
      121 tatcattaat accgccttta aagttatcga aaccatacgt cccaaactgg cctgtagccg
      181 gtgtgatgtc atcgttcagg caccacttcc ccctaaaccg atcgaacgcg gttatgccag
      241 tgcagggtta cttgcacgga tcctggtcag caaatatatg gaacatatcc ctttatatcg
      301 ccagtcagaa atatacgcgc gacagggcgt ggagctgagc cgtaatacca tggtgcgctg
      361 ggtatcagaa atggcagaca aactccgtcc tctgtatata gcgctgaatg actatgttct
      421 ggaggcagga aaggtgcacg cagatgacac tccggtgaaa gtactggccc cggggaacgg
      481 aaagacgaaa acgggtcgtc tgtgggtata cgtcagggat gatcgtaatg cgggttcatc
      541 cctgccggca gccgtctggt tcgcgtattc ggcagatcgc aaaggagaac atccgcagct
      601 ccacctggca aagtatcagg gcgtactgca ggctgatgcc tatgcaggtt ataacgtact
      661 gtacgaaacg ggccgggtga aggaagccgg gtgcctggcc cacgcccgcc gaaaaatcca
      721 tgacgaggat gtgcgccgtc cgacagaaat gactcaggaa gcgctcagac ggatagcaga
      781 gttatacgac atagaagcgg agatacgtgg cagtccggca gaggaacggc ttgcagtcag
      841 aaaagccaga agcgtccagt tgatgcagtc gttgtacgac tggatacagt tgcagaggaa
      901 aacgctgtcg aaacatgcgg agatggcgaa ggcgttcgac tatatcctga atcactggaa
      961 tgcgctgaac gagttctgtc gtgacggctg ggtggaaata gacaacaaca tcggtgaaaa
     1021 cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct cagacaaggg
     1081 aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga acgaagtgga
     1141 gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt cgaaccaagt
     1201 gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tccttacgct a
//
LOCUS       DADLSB010000361         1247 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.367, whole genome shotgun sequence.
ACCESSION   DADLSB010000361 DADLSB010000000
VERSION     DADLSB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.367"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..369
                     /locus_tag="JN331_004385"
     CDS             <1..369
                     /locus_tag="JN331_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7262295.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
     gene            369..716
                     /gene="tnpB"
                     /locus_tag="JN331_004386"
     CDS             369..716
                     /gene="tnpB"
                     /locus_tag="JN331_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7262296.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWLVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            736..>1247
                     /locus_tag="JN331_004387"
     CDS             736..>1247
                     /locus_tag="JN331_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7262297.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCR"
BASE COUNT          302 a          349 c          338 g          258 t
ORIGIN      
        1 cctaattttc cttatgaatt taaaatcgcc ttagtggagc agtcactgca gcccggagcc
       61 tgtgtggcgc agatcgcccg tgaaaacgga atcaacgata acctgctctt caactggcgc
      121 catcaatacc ggaaaggtgg cctgctgcct tccggaaaaa atatgcctgc actgcttccc
      181 gtgacgttaa cgccggagcc ggataataaa atcccggctc cggcacagga accagagcag
      241 ataaatacac cgtccgacag tctgtgctgt gagctggttc tgccggccgg aactctcagg
      301 cttaaaggta aactgacgcc ggcgttatta cagatactta tccgcgaaat aaaagggagc
      361 agccactgat gatatctttc cctgcaggtt cgcgtatctg gctggttgca ggtatcaccg
      421 atatgcgaaa tggctttaac ggcctggcct caaaagttca aaacgtcctg aaggatgacc
      481 cgttctccgg gcatctgttc atcttccgcg gacgccgggg tgaccagata aaagtgttgt
      541 gggctgacag tgacggactg tgcctcttta ccagacgcct ggagcggggc cgcttcgtct
      601 ggctggtcac ccgtgacggc aaggtgcacc ttactccggc ccagttatcc atgcttcttg
      661 agggcatcga ctggaaacac ccgaaacgaa cggaacgcgc tggcatccgc atataacccg
      721 ttgtaaagtg aggatatgga cacctcactt gctcatgaga atgcccgcct gcgggcactg
      781 ttgcagacgc aacaggacac catccgccag atggcgaaat acaaccgcct gctctcacag
      841 cgggtggcgg cttatgcttc cgaaatcaac cggctgaagg cgctggttgc gaaactgcag
      901 cgcatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagatactg
      961 gaagcacagg agcgaatcag cgcacttcag gaagaaatgg cggaaacgct gggtgagcaa
     1021 tatgacccgg tactgccatc tcccctgcgc cagtcttcag cccgtaaacc gttacctgcc
     1081 tcacttcccc gtgaaacccg ggtcatccgt ccggaagagg aatgctgccc ggcctgtggt
     1141 ggcgaactca gttctctggg atgtgatgtg tcagagcagc tggagcttat cagcagcgcc
     1201 tttaaggtta tcgaaacaca acgtccgaaa ctggcctgtt gccggtg
//
LOCUS       DADLSB010000362         1246 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.368, whole genome shotgun sequence.
ACCESSION   DADLSB010000362 DADLSB010000000
VERSION     DADLSB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.368"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            28..>1246
                     /locus_tag="JN331_004388"
     CDS             join(28..352,352..>1246)
                     /locus_tag="JN331_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HAY7262298.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRAKKLDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCL"
BASE COUNT          308 a          297 c          377 g          264 t
ORIGIN      
        1 gtttttagac tagtcattgg agaacagatg attgatgtct tagggccgga gaaacgcaga
       61 cggcgtacca cacaggaaaa gatcgcaatt gttcagcaga gctttgaacc ggggatgacg
      121 gtctccctcg ttgcccggca acatggtgta gcagccagcc agttatttct ctggcgtaag
      181 caataccagg aaggaagtct tactgctgtg gccgccggag aacaggttgt tcctgcctct
      241 gaacttgctg ccgccatgaa gcagattaaa gaactccagc gcctgctcgg caagaaaacg
      301 atggaaaatg aactcctcaa agaagccgtt gaatatggac gggcaaaaaa gtggatagcg
      361 cacgcgccct tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt
      421 cgcgtgcgca gttgcacgtc attctcagac gaaccgatga ctggatggat ggccgccgca
      481 gtcgtcacac tgatgatacg gatgtgcttc tccgtataca ccatgttatc ggagagctgc
      541 ccacgtatgg ttatcgtcgg gtatgggcgc tgcttcgcag acaggcagaa cttgatggta
      601 tgcctgcgat caatgccaaa cgtgtttacc ggatcatgcg ccagaatgcg ctgttgcttg
      661 agcgaaaacc tgctgtaccg ccatcgaaac gggcacatac aggcagagtg gccgtgaaag
      721 aaagcaatca gcgatggtgc tctgacgggt tcgagttctg ctgtgataac ggagagagac
      781 tgcgtgtcac gttcgcgctg gactgctgtg atcgtgaggc actgcactgg gcggtgacta
      841 ccggcggctt caacagtgaa acagtacagg acgtcatgct gggagcggtg gaacgccgct
      901 tcggcaacga tcttccgtcg tctccagtgg agtggctgac ggataatggt tcatgctacc
      961 gggctaatga aacacgccag ttcgcccgga tgttgggact tgaaccgaag aacacggcgg
     1021 tgcggagtcc ggagagtaac ggaatagcag agagcttcgt gaaaacgata aagcgtgact
     1081 acatcagtat catgcccaaa ccagacgggt taacggcagc aaagaacctt gcagaggcgt
     1141 tcgagcatta taacgaatgg catccgcata gtgcgctggg ttatcgctcg ccacgggaat
     1201 atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtct
//
LOCUS       DADLSB010000363         1241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.369, whole genome shotgun sequence.
ACCESSION   DADLSB010000363 DADLSB010000000
VERSION     DADLSB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.369"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="JN331_004389"
     CDS             complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="JN331_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY7262299.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
     gene            1019..1198
                     /locus_tag="JN331_004390"
                     /pseudo
     CDS             1019..1198
                     /locus_tag="JN331_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          405 a          179 c          247 g          410 t
ORIGIN      
        1 ttaatcctgt aacttgaatc taattgtgaa aaactctccg gaagagtggt tatctgatta
       61 aaatgaaaat aatattcctt ttgtctgata ttgtttctat cagaaaattg aggaaaagta
      121 accagattat tgcgctgagc acagagaatt tccagtttat ccggtaagtc gggtaatgtt
      181 gtcagcctgt tataataaac atgtaaaaat ttcaattttt cgggaagagc cggtaagtta
      241 tatagcctgt tattttcaac aaataatgat gttaaatggt ttggcaaaac aggaaggttt
      301 tccagttgat tagaacgaac acttaaagtt gttaatgact caggaagttc tggtatcaca
      361 gaaagcctgt tattatcagc attaagttct ttgagaaatg caggcagctt tggcaatgat
      421 gttaatttat tattttctac aatcaaagca gaaatatgct ttggtaattc gggaagagac
      481 gtcaaattta ggtctgataa atttaatact gcttcttgat tctgaaagca ctgggagagt
      541 ctgttaaaag caatgcctcg ctcttccccg ggagaagagt tcttttccca ttcagtcagg
      601 attctcagat aatgttcatt actggttgag tttgagttaa tagaggggga gcaagaaact
      661 gatgagtgtg tattatttac agagaacata atgctaggtc aaccttatat ttgtgagaat
      721 aaatatttaa aattgtatat ttatacaaat ttcacttacc atttccgatg ttaagtttta
      781 atagtttcca ttttacacat aacagaagtt atgttgtaat ttatttattg aggttttcta
      841 tggaattctt tttttattgt gactgcagtc aataataaca ctgaattcaa cattccagtg
      901 agcaaggaaa tgtaatccgg gtaagtatat ttgttttaca tcatatttta gccaagctca
      961 tttattccta ttatagaatg ataaattaaa attactgtaa aaaaacgata cacatttaat
     1021 acgagggagg ccggcagaaa aacggcttgc ggtaaggcag gagaaatgtg tgccgcgaat
     1081 gaagtccttg tatgactgga taaagctcca gaggaaaact ttgtcaaacc atgctgaaat
     1141 ggtaaaggcg ttcaactata tcctgaatca ctggaatgca ctgaatgagt tttgccgggt
     1201 agtacttcca atcattagaa tatgtgcttt tcaatgaagg a
//
LOCUS       DADLSB010000364         1238 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.370, whole genome shotgun sequence.
ACCESSION   DADLSB010000364 DADLSB010000000
VERSION     DADLSB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.370"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>1238
                     /locus_tag="JN331_004391"
     rRNA            <1..>1238
                     /locus_tag="JN331_004391"
                     /product="23S ribosomal RNA"
BASE COUNT          314 a          280 c          382 g          262 t
ORIGIN      
        1 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
       61 caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga
      121 actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaaggc acgctgatac
      181 gtaggtgaag cgacttgctc gtggagctga aatcagtcga agataccagc tggctgcaac
      241 tgtttattaa aaacacagca ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct
      301 gcccggtgcc ggaaggttaa ttgatggggt tagcgcaagc gaagctcttg atcgaagccc
      361 cggtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag
      421 ttccgacctg cacgaatggc gtaatgatgg ccaggctgtc tccacccgag actcagtgaa
      481 attgaactcg ctgtgaagat gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct
      541 ttactatagc ttgacactga acattgagcc ttgatgtgta ggataggtgg gaggctttga
      601 agtgtggacg ccagtctgca tggagccgac cttgaaatac caccctttaa tgtttgatgt
      661 tctaacgtgg acccgtgatc cgggttgcgg acagtgtctg gtgggtagtt tgactggggc
      721 ggtctcctcc taaagagtaa cggaggagca cgaaggttgg ctaatcctgg tcggacatca
      781 ggaggttagt gcaatggcat aagccagctt gactgcgagc gtgacggcgc gagcaggtgc
      841 gaaagcaggt catagtgatc cggtggttct gaatggaagg gccatcgctc aacggataaa
      901 aggtactccg gggataacag gctgataccg cccaagagtt catatcgacg gcggtgtttg
      961 gcacctcgat gtcggctcat cacatcctgg ggctgaagta ggtcccaagg gtatggctgt
     1021 tcgccattta aagtggtacg cgagctgggt ttagaacgtc gtgagacagt tcggtcccta
     1081 tctgccgtgg gcgctggaga actgaggggg gctgctccta gtacgagagg accggagtgg
     1141 acgcatcact ggtgttcggg ttgtcatgcc aatggcactg cccggtagct aaatgcggaa
     1201 gagataagtg ctgaaagcat ctaagcacga aacttgcc
//
LOCUS       DADLSB010000365         1236 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.371, whole genome shotgun sequence.
ACCESSION   DADLSB010000365 DADLSB010000000
VERSION     DADLSB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.371"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..180
                     /locus_tag="JN331_004392"
                     /pseudo
     CDS             1..180
                     /locus_tag="JN331_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            197..715
                     /locus_tag="JN331_004393"
                     /pseudo
     CDS             197..715
                     /locus_tag="JN331_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          377 a          282 c          262 g          315 t
ORIGIN      
        1 aacggaatta tcgtcggtcg tgacagactg gcacgtcttc gtaaggagct gaggctgcac
       61 tgtaagcaga aacgcaagtt cagggcgacc acaaactctg accacaatct gccggttacg
      121 ccgaatctgc tgaatcagaa ctttacgccc acagcaccta atcaggtctg ggtcgcagat
      181 tcagttgtcc aggccttccg aaatcaaccg actgaaggcg ctggtcgcga aactgcagcg
      241 tatgcagttc ggtaaaagct cagaaaaact tcgcgcaaaa accgaacggc ggatacagga
      301 agcacaggag cgaatcagcg cacttcagga ggaaatggcg gaaacactgg gtgagcaata
      361 tgacccggta ctgccatctt ccctgcgcca gtcttcagcc cgtaaaccgt taccagcctc
      421 acttccccgt gccccccggg tcatccggcc ggaagaggaa tgctgtccgg cctgtggtgg
      481 tgaactcagt ccactgggat gtgatgtgtc agagcagctg gagcttatca gcagcgcctt
      541 taaggttatc gaaaaacaac gccctaaact ggcctgtcgc cggtgcgacc atatcgtgca
      601 ggcaccggtg ccttcaaaac ccattgcacg cagttatgcc ggagcggggc ttctggccca
      661 tgttgttacc ggaaagtatg cagaccatct gccattatac cgccaaagtg atctactatt
      721 ccacacagct atttagctct ctttttgttg agctcccatg attctttcat ttattgttac
      781 taattatttt atgttatcat gatgttagaa cacgctctgc tctgtgtctc tattttatct
      841 atcatattct gaggattaac tacataacaa ggcgcaagat atatatccaa aaacaaataa
      901 taccgttgca ataattcctt tcagtaaagg aggtgtgatt aatttcaggg gatttcagag
      961 tgaaaattgt acaacatatt aaaatatgtt catttcgtta taatacacac aagaaaatat
     1021 taagttagat aatatattct aaataaccat agataaaaac gcacataatg gggaatattt
     1081 tatgaaaata cctgaagcag taaatcatat taatgttcag aataatattg atcttgttga
     1141 tggaaaaacg aatccgaata aagcaacaaa agcattacaa aaaatatatt acgcgtaaca
     1201 aactcatctt cttctggcat aagtgaaaaa cattta
//
LOCUS       DADLSB010000366         1234 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.372, whole genome shotgun sequence.
ACCESSION   DADLSB010000366 DADLSB010000000
VERSION     DADLSB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.372"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(41..>1234)
                     /locus_tag="JN331_004394"
     CDS             complement(41..>1234)
                     /locus_tag="JN331_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY7262300.1"
                     /translation="VFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHL
                     CPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAANMPLYQLL
                     GGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYGLAKGKGL
                     AYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPIEAARF
                     GKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDY
                     IRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQE
                     YMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLPVARLEDG
                     TLLNW"
BASE COUNT          326 a          338 c          282 g          288 t
ORIGIN      
        1 ctaaatcagt aagttggcag cattaccaag cgaaccgtta ttaccagttc aacaatgtgc
       61 catcttccag tcgtgcgacg gggagatacg ccgggtcata cggatatttc gccgccagtt
      121 tctcatcgaa agaaataccc agccccggtt catcgcctgg gtgcatataa ccttcctcga
      181 agcgccagct gtgcgggaaa acttcaagca tttgttcgga atagcccatg tactcctgaa
      241 cgccaaaatt tggtacccat aggtcaaaat gcagcgccgc cgcatgacaa acaggcgata
      301 aatctgaagg gccatgcgaa ccggtacgta cctgataaag cgatgcaaaa tcggcaatcc
      361 gccgcatccc ggtaatgccg cctgcatggg tgatcgtcgt gcgaatatag tcaatcagct
      421 gctcttcgat taactgcttg cagtcccaga tactgttaaa cacttcaccg accgcaatcg
      481 gcgtcacggt atgctggcga atcagacgaa aacattcctg gttctcggca ggcgtcggat
      541 cttccatcca gaacaggcgg taatcctcaa tgctcttccc aaagcgcgcc gcttcgatgg
      601 gggtcaggcg atggtgcatg tcatgcaaca gatgttcgtt aaaaccgaac ttattacgca
      661 ccgcttcgaa taacttcggc gtaaaatcga gatacttttc tgaagaccac aactgctctt
      721 ctggccacag gcctttggtt gccggttcat acgccagtcc tttgcccttc gccaggccat
      781 aagtggtttg catacccggt acaccgcact gcacacgaat cgccttaaag cccatctgct
      841 ggtgctttgc atagtcttca aggacttcat caatcgtacg cccggtggtg tggcaataaa
      901 ccatcacgcc ttcacgagaa gcaccaccaa gcagttgata caatggcata tttgccgctt
      961 tagctttgat atcccacaac gccatatcaa tggcagaaat ggctgacatc gtgacggggc
     1021 cacgtcgcca gtaagcacct ttataaaaga attgccagat atcttcgata cggctggcat
     1081 cgcggccaat tagttgtgga caaagatgat ctttcagata agaagcgacc gacaattcgc
     1141 gaccatttaa cgtggcatct cctaatccac aaagtccgct ttcagtggtt attttaagcg
     1201 taacgaaatt acgccctggg caagtaacaa aaac
//
LOCUS       DADLSB010000367         1228 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.373, whole genome shotgun sequence.
ACCESSION   DADLSB010000367 DADLSB010000000
VERSION     DADLSB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.373"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..131
                     /locus_tag="JN331_004395"
     CDS             <1..131
                     /locus_tag="JN331_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7262301.1"
                     /translation="DREKLKAYRIYAKSLQAMDFSVITDKTSYNAIEWPASPEISS"
     gene            155..>1228
                     /locus_tag="JN331_004396"
     CDS             155..>1228
                     /locus_tag="JN331_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7262302.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIG
                     GLIIAAYQGEADGDKVIRLVRGQS"
BASE COUNT          325 a          300 c          341 g          262 t
ORIGIN      
        1 aagaccggga aaaactgaaa gcgtaccgca tttacgcgaa atcgctgcag gcgatggatt
       61 tcagtgtcat cactgataaa acctcataca acgccattga atggcccgct tctccggaaa
      121 tttcttcctg atttaattta tcgcgagaaa aactatgtct gtagtgatat caggtgcgct
      181 gactgatggc gcaggtatcc ccatgtccgg ataccatatt attctgaaat cccgggtaaa
      241 caccccggaa gtggtgatgc acactgttgc tgatgtgatg acaggaaacg atggtgaata
      301 ctgtttccat gcgcggactg gaaaatatgg tgtgtatctg aaacaggact ggcacaacga
      361 gtacaacgtt ggcgacattg ctgtatatga ggactcaaag cccggcacgc tgaatgactt
      421 tctgattgct cctgatgagg gcgacctgaa accggatgtc gtcaaacgct ttgaggaaat
      481 ggtggcgcag gcgcagcaga gcgccggggc cgcagccgga aacgcacagc agacggcgca
      541 ggatgtggcg gcagccgcag gttatgcccg cgcagcagag caggccaaaa atgacattga
      601 tgctgcgctg accggcactc tgaaaacggc taaccatctg tctgaaatcg cagcagcagg
      661 cgaaaaggca caacagaagt cccgggataa tctggggctg aaaagtgcgg ccacgatgga
      721 agcacagagc gacatttacg accggacaaa aggccgtctg gcgatacccg gcgcattcgg
      781 ctttgggtgt gcttttctgc ctgaagatgt tatccgtttt gacactaaga gtgatttcct
      841 ggcctgggta aggaatgcgc tgccaggtga atattccgtt gctggcccct acggcatcat
      901 catacccgac acacggtttg aaggggtgct cagcatccgg tggactgatg cacgccctga
      961 gacaacagaa ccgaggtaca gagccaaatc ccttactttt tacggcatta acggccccat
     1021 ttatcacacc cgctactgct actggcccat atccagactg actggctggg tgaaaataaa
     1081 tataaccaca gaagatatta tttacagaat cgtggcgagc tctgtccgca acagatgggg
     1141 agaccctgac attggcgggc tgattattgc tgcgtaccag ggagaagctg acggtgataa
     1201 agtcatcaga cttgtcaggg ggcagtca
//
LOCUS       DADLSB010000368         1199 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.374, whole genome shotgun sequence.
ACCESSION   DADLSB010000368 DADLSB010000000
VERSION     DADLSB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.374"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JN331_004397"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JN331_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY7262303.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          341 a          214 c          259 g          385 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagtctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaataatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtc
//
LOCUS       DADLSB010000369         1188 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.376, whole genome shotgun sequence.
ACCESSION   DADLSB010000369 DADLSB010000000
VERSION     DADLSB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.376"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(36..545)
                     /locus_tag="JN331_004398"
     CDS             complement(36..545)
                     /locus_tag="JN331_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY7262304.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            674..1003
                     /locus_tag="JN331_004399"
     CDS             674..1003
                     /locus_tag="JN331_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY7262305.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGCLTIIADSDEVQELREELYQVKQVVKGMRDGM
                     FSVLNES"
BASE COUNT          348 a          248 c          307 g          285 t
ORIGIN      
        1 acgtaaatcc tggcgaaacc aggccagatg ggtagtcata aaactcctga atggcgcaag
       61 ttaatcacac gcggccaaag ttcgaaataa cgctgccctg aaagccaggc gtcaggataa
      121 agcgccatat agtgtttgat acgcatcagc ggcgcaagaa gtggttgctc accacggcga
      181 taactgtcga taagcgcagc cagctgctgg cgttctgtgg aatcaatatg cgggcgaaaa
      241 taaccctgaa cgtgcatcaa aacgttggtg tgatcgcgcg ggttggcggg atgagaaagc
      301 aaaacaatta cgcgttggcg gtactggttg taaaaatcat ccagattttg ccactgatga
      361 atatcagcca caaacgggcc aagtttgcgg tactcgggct gagaatgggc cagaaagacg
      421 agtttatagc ggctgtgata atcgagaagc gccccgcgcg ttaagccatg ttctttcagt
      481 cgatgtaact catgaagggc gaagatacgg attagggtgc tgtcatcaaa tcgttgtaga
      541 ttcattactg ctcctgaacg gtatacagga gtaagtgtag acgttggtaa atgatgtttg
      601 tgaagttaat ccgctagcag ggcaagatta cgaaagcgcg ctccccgcaa gcaactaatc
      661 aaggtagtta atcatggctg aacacgatat tacgccagaa gtcaccattt ccaaaacaca
      721 gcgccgctat aaagtcggct atgtcagagc ccgccaccag gaccgcaaaa ccggtatagc
      781 ccggtattat tcacaacatc ccagcctgca ccttaagggc gactggctga aagaggccgg
      841 atttgatacc ggacggggtg tcacggtgaa gatttcggag gggtgcctga ccatcatcgc
      901 tgacagtgac gaggtgcagg aactgcggga ggaactttat caggttaaac aggtagttaa
      961 ggggatgcgg gatgggatgt ttagtgtgct gaacgaaagt taaatattaa aaaagctggg
     1021 aagatttaaa accttcctag ctttttattc tgtcttatta taatggatac tcatcatctt
     1081 ccgatgaatt tataaattgc tccaagttta catgaagagt acttaaccta gtttttaact
     1141 cattaagtga agcgataatt tcatattttc caacataatc atatgatg
//
LOCUS       DADLSB010000370         1145 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.377, whole genome shotgun sequence.
ACCESSION   DADLSB010000370 DADLSB010000000
VERSION     DADLSB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.377"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            317..1066
                     /locus_tag="JN331_004400"
     CDS             317..1066
                     /locus_tag="JN331_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7262306.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          340 a          222 c          225 g          358 t
ORIGIN      
        1 acctcaaaaa gttatcggtg cgtttatcga aaaacatatg ttctactaat tggagtcatt
       61 gtctgtaaaa gcaactcaaa aatacaagtt gcttattccc cttgttggta agaacttaat
      121 tctaactgtt tgttctgggt gttttaaacg tcctcctttt tatgcttgtt cataacggat
      181 atttaatagg taacctttgt tttagcatct tctgatttat ttaaattatg tgatagcgca
      241 cacaatttta ttatccagta ctttatactc ttaataaaat aattataaat atttatcatc
      301 tataaggaga tgttatatga aaagacagct ttttactctt agtattgtcg gggtattttc
      361 tttaaatacc tttgcatcat tccctccggg aaatgatgtg acaacaaaac ctgaccttta
      421 ctacctgaca aacgataatg ctattgacag tctggcatta ttaccgcctc caccacaaat
      481 cggaagtatt gccttcctga atgaccaggc catgtatgaa aaggggcgtt tattgcgaaa
      541 tactgaaaga gggaagctgg cagctgagga tgccaatctc agtagtggtg gcgtggcaaa
      601 tgtattttct gctgcttttg gttctccgat aacggctaaa gattcaccag aattgcataa
      661 attactgaca aatatgattg aggatgccgg agacctggca acgcgttcag caaaagaata
      721 ttatatgcgt attcgacctt ttgcttttta tggtgtttct acgtgtaata caaaagaaca
      781 ggatacatta tccagaaatg gctcttatcc atcaggtcat acatcgattg gttgggcaac
      841 agcacttgta ttgtccgaaa taaatccggc acgccaggat accattctga aacggggtta
      901 tgaactgggg gacagcaggg ttatttgcgg ttatcactgg caaagtgatg ttgacgcagc
      961 acgtattgtt ggctctgcta ttgtagcaac actgcactca aatcctgtgt tccaggcaca
     1021 attacaaaaa gcgaaagatg aattcgctaa caatcagaaa aaataatgct tttagccagt
     1081 acgccttata accccgacct tcagggctaa tgccagtcag ttaagcaact gactggctct
     1141 ttttc
//
LOCUS       DADLSB010000371         1142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.378, whole genome shotgun sequence.
ACCESSION   DADLSB010000371 DADLSB010000000
VERSION     DADLSB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.378"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            293..1135
                     /locus_tag="JN331_004401"
     CDS             293..1135
                     /locus_tag="JN331_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262307.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
BASE COUNT          388 a          196 c          221 g          337 t
ORIGIN      
        1 ctgtagatcg ctgtgtgttg catctatcgg ttgaactcac agcccaaacc agactgtcag
       61 ctttgattag attctaccta ctcaacctgt cagataaggt ctgagctaat acatctaata
      121 tatggctttg tgcaggggac ggctatcgcg aacgttaggc taggtatctg aatacgcaca
      181 gttcacttgg ttgtacttct tgcactgatc tgctatcccg cacagctatt tataggtaat
      241 cttcagtgta taatctgccg acttatggaa gcttgtacaa caggagaaat atatgaaaaa
      301 agaattgttt ggacgttgcc cgtttgatct cgacgaagat gtaaaaaaaa tagccgcgat
      361 aaataaatat atccatgcaa aatttgagga gcataaaaat aaaatagaca acctaaaggg
      421 tgtggaacaa aaattgatgg tcttacagta ctttaatgta ctgaatgaac ttagtaatca
      481 ggctatctct tctttgaata taggagcctt ttcagcatcg gaagtattag ctagggttat
      541 aatagaacag gctgccaatc aattttatat agcaatagat gatggcgaaa atgcacaagc
      601 attactcaag gctagtaaga aattagttca tagtaatgga aaacggtggc tggagtgttt
      661 aaaatccaaa ggaatgacta atccagcagc aaatgaaagg attcgcatgg gaaaagaact
      721 aactgatcta tttaatagat tgtggccaaa tacacctgaa tatccaggaa ccagaaaact
      781 ttttgaatta attggctggg aaacacacta tcatgcccat tacgttcccc tttgtgattc
      841 aatacataca ttctctgatg atatggtaaa tatagtatct ctatataatg caatccaaag
      901 tgatgaaact actgctatcg aattcattct tgcagttaaa caagaaaata aacggctggc
      961 aatttataat tttgtaattg ctacggccct tcggtgtgag gccttagtaa atgtatttaa
     1021 ctctttaggt tatcaggata ttataacaga aatgacacca acaatagata cagtcaacca
     1081 gactataatt cgatatgatg attttgatca ttccagaatt gatgattgtc aataattaat
     1141 tg
//
LOCUS       DADLSB010000372         1139 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.379, whole genome shotgun sequence.
ACCESSION   DADLSB010000372 DADLSB010000000
VERSION     DADLSB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.379"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(1..265)
                     /locus_tag="JN331_004402"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="JN331_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /locus_tag="JN331_004403"
     CDS             complement(380..1018)
                     /locus_tag="JN331_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262308.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          332 a          280 c          174 g          353 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactctt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tgttttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gagtctaaa
//
LOCUS       DADLSB010000373         1135 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.380, whole genome shotgun sequence.
ACCESSION   DADLSB010000373 DADLSB010000000
VERSION     DADLSB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.380"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(11..202)
                     /locus_tag="JN331_004404"
     CDS             complement(11..202)
                     /locus_tag="JN331_004404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262309.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASLAEVYWQ"
     gene            339..1135
                     /locus_tag="JN331_004405"
                     /pseudo
     CDS             339..1135
                     /locus_tag="JN331_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          300 a          293 c          328 g          214 t
ORIGIN      
        1 gtagtgtccg ctattgccag tacacctcag ccagggatgc cggcacatct gccagtgagg
       61 cggcaaccaa tgcgactgat gctgcaggct cagcacgcgc agccagcacg tcagccggtt
      121 cggcagcgtc atcggcatca tctgcgtctg cttcaaaaga tgaggcgact aaacaggcgt
      181 cagcagcgaa gggcagtgcc acgacggcat cctctaaggc gacagagtct gcaggcagtg
      241 cgacggcggc agcgcagagc aaaagcgcgg cagaatccgc ggcaatgcgc gctgagacag
      301 cagcaaaacg ggcagaggat attgctgatg tcgtatctct ggaagatgcg agcacgacga
      361 aaaaggggat agtgcagctc agcagtgcga ccaacagtgc atccgagtca ctggccgcga
      421 cagcgaaagc ggttaaggtg gtaatggatg aaacaaacaa gaaagcgccc ctgaacagtc
      481 cggctctgac cggaacgcca accacaccca ctgcgccaaa ggggactaat aatactcaga
      541 tcgccagtac ggcttatgtt atggccgcga ttgctgccat cgtggactcg tcgcctgatg
      601 cactgaacac gctgaatgaa ctggcggcgg cgctgggcaa cgacccgaat tttgcgacca
      661 ccatgactaa cgcgcttgcg ggtaagcaac cgaaagatgc cactctgacg gcgctggcgg
      721 ggcttgctac tgcggcagac aggtttccgt attttacggg gaatgatgtc gccagcctgg
      781 caaccctgac aaaagtcggg cgggatattc ttgcgaaatc gaccgttgcc gccgttatcg
      841 aatacctcgg tttacaggaa acggtaaaca aggctgataa cgctgtccaa aagacaggcg
      901 ataccttgtc cggtggactt acttttgaaa acgactcaat ccttgcctgg attcggaata
      961 ctgactgggc gaagattgga tttaaaaatg attcggatgc agacaccgat tcctacatgt
     1021 ggtttgaaac ggcggataac ggtaatgaat atttcaaatg gagacacgcc tcgctggcgg
     1081 ccgggttaaa gacctgatga atctcaaatg ggatacacta aatattctgg ttaat
//
LOCUS       DADLSB010000374         1114 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.381, whole genome shotgun sequence.
ACCESSION   DADLSB010000374 DADLSB010000000
VERSION     DADLSB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.381"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..261
                     /locus_tag="JN331_004406"
     CDS             <1..261
                     /locus_tag="JN331_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7262310.1"
                     /translation="DGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQ
                     FKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            263..>1114
                     /locus_tag="JN331_004407"
     CDS             263..>1114
                     /locus_tag="JN331_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262311.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSNEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSH"
BASE COUNT          347 a          200 c          225 g          342 t
ORIGIN      
        1 gatggttcga tccgctttaa caataagatg ccttatcacg ttacgctggg ttatatcgga
       61 acaaacggaa tcacaatgtt gccacaaacg caaagtttaa tggtaacccc ttatagccat
      121 gcagatacgc agtttaaaaa tgttccgtca gcttttcagg ttggttatat caatgatttt
      181 ggtggattaa gtttttatga aataaactgc ccgacggtta ataactcttg taatgtttcc
      241 gtagccaaac gagataaata atatgcgcta cttattaatt gtaattatat ttattatagg
      301 ttttagtgca ttacctgtat gggcaatgga ctgctatgct gaacatgaag agggaaatac
      361 cgtagtcatt ggttacgtac caagaatttc tatccccagt aatggtaaaa aaggtgataa
      421 gatctggcaa agcaatgagt attttatgaa tgtcttctgt aataatgcat tacctgcccc
      481 atcacctgga gaagaatacc catcagcctg gactaatata atgatgtttt taccaggcgg
      541 tcaggacttt tataatcaga actcctatat ttttggcgta acatacaatg gagttgatta
      601 tgattcgact gcccctaatg ctatcgcagc accagaatgc attgatataa aaggagcggg
      661 tacctttaat aatcactata aaaaccccgc tgtctgtagt ggaggtcctg agcctcaatt
      721 atcagtaacc tttccagctc gcgtacaact ctatattaaa ttagcaaaaa atgccaatag
      781 agttaataaa aacctggtgt tgcctgatga atatatcgct ctagagttta aaggtatgag
      841 tggcgcagga gctatagaag tagacaaaaa tttgaccttc aggattcgtg gattaaataa
      901 tattcatgtc cttgactgct ttgttaatgt cgcactggaa ccagctgatg gcgttgttga
      961 ctttggtaaa ataaattccc gaacaattaa aaataccacc gttagtgaga cgtttagtgt
     1021 agtcatgaca aaagatcctg gtgcggcctg tactgagcaa ttcaatattt tagggagctt
     1081 tttcactacg gatattttga gtgattatag ccat
//
LOCUS       DADLSB010000375         1086 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.382, whole genome shotgun sequence.
ACCESSION   DADLSB010000375 DADLSB010000000
VERSION     DADLSB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.382"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>1086
                     /locus_tag="JN331_004408"
     CDS             <1..>1086
                     /locus_tag="JN331_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262312.1"
                     /translation="LSDGKNENGQLFDKQKALKRIKELEGEQIKTARREMVLAVVGTM
                     KAGKSTTINAIVGQEILPNRNRPMTSVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQ
                     EKLATPAGQQVAQTLQQTGDTRELLDILTDDGWLKNEYHGEEEIFTGLASLNDLVRLA
                     AAMGSEFPFDEYAEVQKLPVIDVEFSHLVGMDECQGTLTLVDTPGPNEAGQPQMEVMM
                     RDQLQKASAVLAVMDYTQMNSKADEEVRKELNAIADVSVGRLFVLVNKFDEKDRNGDG
                     ADAVCQKVPAMLNSDVLPASRVYPGSSRQAYLANRALHELRKNGTLPVDEAWVDDFVR
                     EAFGRMKKDYVCKDSELATEGATDLWE"
BASE COUNT          294 a          264 c          307 g          221 t
ORIGIN      
        1 tgttatctga cggtaagaac gaaaacgggc agctttttga taaacagaaa gcactgaaaa
       61 gaattaagga gctggagggc gaacaaatca aaaccgcccg cagggagatg gtgctggctg
      121 ttgtcggcac gatgaaagca ggcaaatcaa ccaccatcaa cgccattgtg gggcaggaaa
      181 ttctgcctaa ccgtaaccgc cccatgacct ctgtaccgac gctcatccgc cacgttcccg
      241 gaaaaactga gccggttctc catctggaac atattcagcc tgtccgcaat ttattaatca
      301 cactgcagga aaaactcgcc accccggcag gacagcaggt cgcacagacc ctgcagcaaa
      361 ccggggatac ccgcgaactg ctggatattc tgacggatga tggctggctc aaaaatgaat
      421 accacgggga ggaggaaatc tttaccggac tggcatcgtt aaacgatctg gttcgtcttg
      481 ctgcggcaat ggggagtgaa tttccttttg atgaatacgc agaagtgcag aaactgccgg
      541 tgatcgacgt ggaattcagc catctggtgg ggatggatga atgccaggga acactcacac
      601 tggtggatac ccccggccct aatgaagccg gacaaccgca gatggaagtg atgatgcggg
      661 atcaactgca gaaagcctct gcggtgctgg cggtgatgga ctacacccag atgaactcga
      721 aagccgatga ggaggttcgt aaagagctta atgccattgc tgatgtgtca gtcggccgcc
      781 tgtttgtgct ggtcaataaa tttgatgaaa aagaccgtaa tggcgatggg gctgatgcag
      841 tctgtcagaa ggtaccggca atgctgaaca gcgatgtgct gcccgcctcc cgcgtttatc
      901 ccggatcctc acgccaggca tacctggcta accgtgcgct tcatgagtta cggaaaaacg
      961 gaacccttcc tgttgatgaa gcctgggtcg atgattttgt cagggaagcc ttcggtcgca
     1021 tgaaaaaaga ttacgtctgt aaagacagcg aactggcaac tgaaggggcg acagacctgt
     1081 gggaag
//
LOCUS       DADLSB010000376         1078 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.383, whole genome shotgun sequence.
ACCESSION   DADLSB010000376 DADLSB010000000
VERSION     DADLSB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.383"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..390
                     /locus_tag="JN331_004409"
                     /pseudo
     CDS             1..390
                     /locus_tag="JN331_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343264.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            288..710
                     /locus_tag="JN331_004410"
     CDS             288..710
                     /locus_tag="JN331_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7262313.1"
                     /translation="MRQTEDKQHQNSEPEEGTPCNQTGEDNCPDCGAVMGDATYQETF
                     DEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGL
                     CANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            688..984
                     /locus_tag="JN331_004411"
     CDS             688..984
                     /locus_tag="JN331_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY7262314.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
BASE COUNT          348 a          238 c          274 g          218 t
ORIGIN      
        1 aggcaacggc tgaaacagtg gaaccgaatg caactgaaca tcatcagaac acgcagccgc
       61 tggatgctca gtcacaggta aattctgttg atgagaaata tcaggaactg cgggcagaac
      121 tccatgaagc ccggaaaaac attccgctta aaaatcctgt cgatgcagac aaattactgg
      181 ctgcctctcg cggagaattt gttgaaggga ttagcgaccc gaatgattcg aaatggattc
      241 ccggtcatca tatctcctca aatgaagtca aaaaaacgga aaatgaagtg cgacagactg
      301 aagataaaca gcaccagaac agcgaaccgg aggagggaac accctgcaat cagactggcg
      361 aggataactg ccctgactgt ggcgcggtga tgggagacgc aacttatcag gaaacttttg
      421 acgaaaaaaa accagatgaa gcacaggaag aagagccgaa gaaaacggaa aaagctgatg
      481 atttgttacc ggagaacgct ggcagcgatc agcataatga tagcaataat gaaactggcg
      541 aagatgaact caactggaaa aaacaggtgt tgattgcggc ggtttacggt ttatgtgcga
      601 atcctgcgtg catagcctat gcgcctgcaa tccctgacat cgcaatgatg attgccaaca
      661 agcttgaaaa ttttggagtg accagtgatg agcgcttacc cgatttttga tcgtattgaa
      721 gaaatggcat gggctcgcca ctaccagcag atcgttcgtg aagaaaaaga aacggaactg
      781 gcggacgacc tggaaaaagg tctgcctcag cacctgtttg aatcgctctg catcgaccat
      841 ttgcaacgcc acggtgccaa taaacaggca atcagccacg catttgatga tgatgttgag
      901 tttcaggagc gcatggcaga acacatccgg tacatggctg aaaccattgc ccaccaccag
      961 gttgatattg atttggagga ctaaaatcta tggcgctttt tcaacgagca ataaacacac
     1021 aggcctatct aaaagcagga attatggggt ttgctggcga cggcaaaacc tacacagc
//
LOCUS       DADLSB010000377         1044 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.384, whole genome shotgun sequence.
ACCESSION   DADLSB010000377 DADLSB010000000
VERSION     DADLSB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.384"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(599..673)
                     /locus_tag="JN331_004412"
     tRNA            complement(599..673)
                     /locus_tag="JN331_004412"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(638..640),aa:Gly,seq:tcc)
     gene            complement(677..751)
                     /locus_tag="JN331_004413"
     tRNA            complement(677..751)
                     /locus_tag="JN331_004413"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(716..718),aa:Thr,seq:tgt)
     gene            complement(754..830)
                     /locus_tag="JN331_004414"
     tRNA            complement(754..830)
                     /locus_tag="JN331_004414"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(794..796),aa:Arg,seq:tct)
     gene            complement(832..908)
                     /locus_tag="JN331_004415"
     tRNA            complement(832..908)
                     /locus_tag="JN331_004415"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(872..874),aa:Ile,seq:cat)
BASE COUNT          293 a          262 c          235 g          254 t
ORIGIN      
        1 attgtatgaa aaaacacgca cgactaattg acaataattt tcatttccat taaaaaagat
       61 cgcgtagcat tcttaattca tgaggaactt tacccgccag caatctgagt agcgaaagct
      121 gttcatcccc aatgtttttg ctggcgggtc cttttttcta cggtcctcct ccccggaggg
      181 gggggcattt cattattttt ctaatcgtta ctggtgagaa ggcggaagaa aaagccagtt
      241 cttcgcccct aaataacagc atgattccag tccatgcaaa atctgatttc cagctcacac
      301 ataccgagca ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt tcccggggtt
      361 tgcttagaat attttgtaag ttgtccgaag agatatttac aacaccagaa tgatgcatta
      421 tcagtcccct gccagaggtt cactacgtaa cctgaccgac aatgcaccat tctggtgctg
      481 aaaaaagagc actggaactg caacaaaaca acatcacaga acaggagatg gtaaggagtc
      541 agacattccc acacaatatt gtgtcagtgc aaataacaac cttcgtctca aatctcgctg
      601 gagcgggcag cgggaatcga acccgcatca tcagcttgga aggctgaggt aatagccatt
      661 atacgatgcc cgcatatggt gcgactaccg gaatcgaact ggtgacctac tgattacaag
      721 tcagttgctc tgcctgctga gctaagtcgg cgctggccca ccaccgagga ctcgaacctc
      781 gcaccgtcaa cttagaaggt tgatgctcta tccggatgag ctagtggtgg ttggtggccc
      841 ttgctggact tgaaccagcg acctggcgat tatgagtcgc tcgctctgac caactgagct
      901 aaagggccgg aggcagaata ataaccatat gtcatcacat ctgcaaactc atctgaccac
      961 cagcgcgttt aacgccctgt gccatttttc aggcacaaaa aaacccgcat aaagcgggtt
     1021 ctttcaagtg tccatgtctg ctat
//
LOCUS       DADLSB010000378         1040 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.385, whole genome shotgun sequence.
ACCESSION   DADLSB010000378 DADLSB010000000
VERSION     DADLSB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.385"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            92..274
                     /locus_tag="JN331_004416"
     CDS             92..274
                     /locus_tag="JN331_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAY7262315.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     RVTELFALLHTEEGCC"
     gene            complement(375..704)
                     /locus_tag="JN331_004417"
     CDS             complement(375..704)
                     /locus_tag="JN331_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY7262316.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
BASE COUNT          269 a          237 c          238 g          296 t
ORIGIN      
        1 ccgttcagac ggcaggtgcc tgcttttccg gcaacaagca ggaatctcag cgctgaagca
       61 ggaattagag gtaaaaacgc cctaccgggc catgaatcac ccggtcattg gagtagtaac
      121 caaagcagat ttagccagca tggaacaaat atctttggtg aaaagttggt tacgggaagc
      181 tggagcgcac aacgtgttag taaccagtgc agttaataac aatagagtta cagaactttt
      241 tgccttgctg catacagaag aaggctgttg ttaatttcga ttggttctca gatgagaatg
      301 atgacggtga tacacagata agaatcaatg agcaatgagg gttgccgggc aaccctcatt
      361 gaataaaacg ggaattactt cgcttcgccg tttttcattt cgcccatagc tttcagcttt
      421 ttggagatat cgcggcgttc tttggagagc tcggcatttt tgatgatgta atcgtcaacg
      481 cgatcttcat agtcaccttt catgctggca atgatgccct ggattgcttc aacgctcatc
      541 cctggcttga tgtaatcgct caggttgtcc agcaacagga cgcgcttctg gttgtcacgg
      601 atctttttct caacgtcctg aatttcacgt tgcagtttgt tcttacgacg gaacagacga
      661 acaaattcca gtacgtcctg gaatgaaggc ttggtagttt ccatttttat acccctgata
      721 atgtgagagt cggattcgtt taagcaaccg ctattcgtta gggccaacat tactcatagc
      781 tgccgcagat gacaatgctt ttatcccttt tcactatcat accctttatc cttgctgaat
      841 cgaagcagca gcaagatgat tctgaagttc aggaattatt tgcgcaaggc ccggcaaatc
      901 agattcgata gcaactcaag ctgatgtgcc gtttccatgt tcagccacac ataaccatag
      961 attggtgttt ccacgacttt caggtcatgg tggttatcga gcaactgacg cagctcttcc
     1021 acagcatcgt tcaatttttc
//
LOCUS       DADLSB010000379         1035 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.386, whole genome shotgun sequence.
ACCESSION   DADLSB010000379 DADLSB010000000
VERSION     DADLSB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.386"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..584)
                     /locus_tag="JN331_004418"
     CDS             complement(<1..584)
                     /locus_tag="JN331_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAY7262317.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPT"
BASE COUNT          308 a          235 c          208 g          284 t
ORIGIN      
        1 gttgggcagg cgtttgcgca ttcaccgcat aacacacatt tcgaggattt tttactttcg
       61 gtatttacgg tggccatcat ccacgggcat gccgtagtac aggcgctaca gccaatacaa
      121 cgtttatgat cgacggtaat acagccttct ttctgctgcc aggtaatcgc accaatcggg
      181 cagacgttca tgcattgcgg ctctttgcat tgacggcagg tgtccgcggt atagttgaga
      241 tcgccataca ggccgccgcc agagccaacc ccgttgtcgc caaagaaata attgcgatgg
      301 attttgatac gggagaagaa tgtccctact gagccatcgt tgaagttggt acaagagatt
      361 tcacagcggt ggcaacccgt gcatcgcgct cgctgggtca ccagcacccc tttgggtgta
      421 tttatcaacc ctactgtgcc gctatcaata tcttcctgtt tgcaacccaa aagtgacagt
      481 aacgcagggg caattgttag accagcaagg ccttttcctg atatacgcaa gaattctaat
      541 cgcgttaagc caatatctaa tagtggacga tcaaccgggt tcatttattt cgcatcctct
      601 tcgcaaacac agtgctgaca attcagcagc ctgatattaa gcattcaact aatacgctgc
      661 tgactctgtc cccaacacca gatattttcc ctaacccgat agtgtatgag aaaagattta
      721 acgccgataa actaatcctt tatggttagg agaaaaaata atgtgattat tctgagccct
      781 taacattgat cgttatcaat taaaattaca aagcaagaaa ttataagcga actgatattt
      841 attatcattt gaaataaatt taacttaagc gacaatcatt tgtgacgttc attgcagaaa
      901 ataaaaataa cattatcgct ataaattaaa atacataccg aaattcgtat aatttcagca
      961 gttgttattt agaatgatta actggaccgc aactgaagca agaatatcag gatgaagctt
     1021 accgcctcat cctga
//
LOCUS       DADLSB010000380         1012 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.387, whole genome shotgun sequence.
ACCESSION   DADLSB010000380 DADLSB010000000
VERSION     DADLSB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.387"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            7..>1012
                     /locus_tag="JN331_004419"
     CDS             7..>1012
                     /locus_tag="JN331_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7262318.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     K"
BASE COUNT          220 a          323 c          260 g          209 t
ORIGIN      
        1 gacagaatga ccgaatccag cgattacgaa tccgtccagg tctttatcgg cgttgatgtc
       61 ggtaaagata cgcatcacgc tgttgccatt aatcgttcag gtaaacgcct gttcgataaa
      121 gcattaccca acgacgaaaa caaactcagg tcgctaatat ctgacctgaa acaacatggt
      181 cagatactgc tggttgttga tcagccagct accatcggtg cgttacctgt cgccgttgcc
      241 cgctcagaag gagtccttgt cggatacctc cctggactgg ccatgcgccg catagccgac
      301 ttacacgccg gtgaagctaa aactgatgct cgtgacgctg ccatcattgc cgaagctgcc
      361 cgtaccctgc ctcacgcgct acgcacgctg aaactggctg acgagcaaat cgccgaactc
      421 tccatgctct gcggcttcga tgatgatctt gccgcacaga caacgcaggc cagcaaccgt
      481 atccgcggcc ttctgaccca gatacatccg gcactggagc gcgttctcgg tccgagactt
      541 gagcacccgg cggtactcga tcttctccag cgatatccct caccagaaaa actcgcttcg
      601 ctgggtgaga agaagctggc agcccagctc tgcaaacttg cgcctcgtct gggtaaacgc
      661 cttgcagcag acatagctca ggcactggcc gaacaaaccg tcgtcgttcc cggcacgaat
      721 gccgctgccg tagtactgcc acgtctggca ctccagctca tcacgctgcg taagcaaaga
      781 gacgaggtgg cgcttgaggt agaacagcga gttcttgctc accctcttta cccggtcctg
      841 accagtatgc ccggagtcgg tgtcaggacc gcagccagac tcctcaccga ggtcgcctgc
      901 cgcgccttcg cctctgccgc acatctcgct gcttatgctg gccttgcgcc ggtaactcgg
      961 cgatccggct cgtcaatacg cggtgagcat ccctcgcgac ggggtaataa ag
//
LOCUS       DADLSB010000381         1011 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.388, whole genome shotgun sequence.
ACCESSION   DADLSB010000381 DADLSB010000000
VERSION     DADLSB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.388"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN331_004420"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN331_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY7262319.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          292 a          163 c          244 g          312 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc a
//
LOCUS       DADLSB010000382         1006 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.389, whole genome shotgun sequence.
ACCESSION   DADLSB010000382 DADLSB010000000
VERSION     DADLSB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.389"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(1..992)
                     /locus_tag="JN331_004421"
                     /pseudo
     CDS             complement(1..992)
                     /locus_tag="JN331_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004969972.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT          299 a          262 c          217 g          228 t
ORIGIN      
        1 tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa ctggctggaa
       61 tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc gatgaatacc
      121 agcgcagcta cttgtcccag ggtattcgca atggcgtaaa tcaccattaa agtattaccc
      181 atgttgtaat actcgcccag cttctgaaag gcgtaatatt gaccgttcgt cattaagtca
      241 tccgggatat tacgcgagtc aaacatcatc cccatcgcca gtgagcccag aatggcacaa
      301 accgcaacca tcaccgccag gcatagcatc ccttttggaa attcttttcc tgggttgcgc
      361 gtttgattaa cgtaaggaga aatcttctct gctccgccaa ccgcgaaaac cagcattgaa
      421 atagtggtaa tgtaggtaaa gtcgatatga ggaatgaacg tttcccaggt aatgtttgtg
      481 gtcgcaatat gcacttcagt aatcgcaggc gcggttaccg ccatcgccac atacaggagc
      541 gacataacaa acatcgcaat ccctgccaca gaaccgacga ttttcagcga tttcataccg
      601 cgtgaagcaa cccacataaa gaagataaac agcaccagcg ttaacccctg taacgctacg
      661 actgaatatt ctttgattag cgaaccgtcg cctttcatcg cccaaccgag cgcaatcaga
      721 attgcctggg gtttttgtgc cagataggga atatgcacca cccagtaggt ccacgcggcg
      781 agataagcca gtccgggtcc catcgtgtgt ttaatccagg tactgacccc gcccttccca
      841 tctttgaagg tcgagcctaa ctgaccaaca attagcgcat aaggtgtgaa atagagtgca
      901 aagataaaca cccatgaaaa aacaaccacc agcccctggt tggcatagtt attaacaacg
      961 ttgccaaaac cccagacagt gataaaagac atcagcgcaa tgttgt
//
LOCUS       DADLSB010000383          991 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.390, whole genome shotgun sequence.
ACCESSION   DADLSB010000383 DADLSB010000000
VERSION     DADLSB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.390"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            81..991
                     /locus_tag="JN331_004422"
                     /pseudo
     CDS             81..991
                     /locus_tag="JN331_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT          300 a          220 c          232 g          239 t
ORIGIN      
        1 gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt tagttactgg
       61 cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat ggcattaacc
      121 gatatcaaag tgaaaaccgc aaaacccaaa gacaaaccct ataagcttgc tgatggcggc
      181 ggtatgtatc tactgattaa cactaatggt tctaaatact ggcgcatgaa gtaccgcttt
      241 gctggtaaag aaaagatgct ttctattggc gtataccccg atgtaacgct agccgatgct
      301 cgcgagaaac gcagtgaagc caggaaactc ctcgctgctg gaggcgatcc aggggaggca
      361 aaaaaggaag aaaaaatcgc gcagcaaatg agtctgaaaa atacgttcga ggctattgct
      421 cgcgaatggc accagttaaa agctgatcgg tggtcattac gctatcgtga tgaaatcatt
      481 gatacctttg aaaaagacat ttttccctac attggcaagc gccctattgc cgaaatcaaa
      541 ccaatggagt tactggaagc actgcgtaaa atggaaaaac gtggagctct ggagaaaatg
      601 cgcaaggtcc gccagcgctg tggtgaggtg ttccgctatg caattgttac tggccgggca
      661 gactataacc cagcccccga tcttgccagt gctttagcta caccgaaaaa agtacatttt
      721 ccctttctta ctgctaatga acttccccac ttcctcaacg atctggcggg ttataccgga
      781 agtataatca ctaagacagc tactcagatc attatgttaa ccggcgtacg aacacaagaa
      841 ctgcgttttg cacgctggga agatatcgat tttgagacaa aattgtggga gatcccggca
      901 gaagtaatga agatgaagcg acctcatatt gtgccgctct ctgagcaggt cattatgcta
      961 tttaagcagc ttgaaccaat ctcaaaacat c
//
LOCUS       DADLSB010000384          987 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.391, whole genome shotgun sequence.
ACCESSION   DADLSB010000384 DADLSB010000000
VERSION     DADLSB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.391"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>987
                     /locus_tag="JN331_004423"
     rRNA            <1..>987
                     /locus_tag="JN331_004423"
                     /product="16S ribosomal RNA"
BASE COUNT          245 a          235 c          307 g          200 t
ORIGIN      
        1 acctttgctc attgacgtta cccgcagaag aagcaccggc taactccgtg ccagcagccg
       61 cggtaatacg gagggtgcaa gcgttaatcg gaattactgg gcgtaaagcg cacgcaggcg
      121 gtttgttaag tcagatgtga aatccccggg ctcaacctgg gaactgcatc tgatactggc
      181 aagcttgagt ctcgtagagg ggggtagaat tccaggtgta gcggtgaaat gcgtagagat
      241 ctggaggaat accggtggcg aaggcggccc cctggacgaa gactgacgct caggtgcgaa
      301 agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgtcgact
      361 tggaggttgt gcccttgagg cgtggcttcc ggagctaacg cgttaagtcg accgcctggg
      421 gagtacggcc gcaaggttaa aactcaaatg aattgacggg ggcccgcaca agcggtggag
      481 catgtggttt aattcgatgc aacgcgaaga accttacctg gtcttgacat ccacggaagt
      541 tttcagagat gagaatgtgc cttcgggaac cgtgagacag gtgctgcatg gctgtcgtca
      601 gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta tcctttgttg
      661 ccagcggtcc ggccgggaac tcaaaggaga ctgccagtga taaactggag gaaggtgggg
      721 atgacgtcaa gtcatcatgg cccttacgac cagggctaca cacgtgctac aatggcgcat
      781 acaaagagaa gcgacctcgc gagagcaagc ggacctcata aagtgcgtcg tagtccggat
      841 tggagtctgc aactcgactc catgaagtcg gaatcgctag taatcgtgga tcagaatgcc
      901 acggtgaata cgttcccggg ccttgtacac accgcccgtc acaccatggg agtgggttgc
      961 aaaagaagta ggtagcttaa ccttcgg
//
LOCUS       DADLSB010000385          986 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.392, whole genome shotgun sequence.
ACCESSION   DADLSB010000385 DADLSB010000000
VERSION     DADLSB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.392"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            188..>986
                     /locus_tag="JN331_004424"
     CDS             188..>986
                     /locus_tag="JN331_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7262320.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG"
BASE COUNT          284 a          204 c          228 g          270 t
ORIGIN      
        1 acaaaaatga aggtactgcc gaaaatattc agatagagct gagggatgac caagatgctg
       61 cgttaaaaaa tggcgatagc aaaacggtta ttcgaggcgc taatcaatgt gatctacacc
      121 tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt taaccttagg
      181 atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc ttctattaat
      241 ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta ttggagcagg
      301 tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga atctggttgt
      361 agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg acactgatca
      421 tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata aaggatcgct
      481 gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg tattggatat
      541 tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag ttggggcggc
      601 cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt ataaaattgc
      661 aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa ataacagtgt
      721 tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcaccgtgg atctcccgga
      781 ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac aaaagctctc
      841 attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta acacagcacc
      901 tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa aaatactggc
      961 tacgggagaa aatgtctccc tgggga
//
LOCUS       DADLSB010000386          983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.393, whole genome shotgun sequence.
ACCESSION   DADLSB010000386 DADLSB010000000
VERSION     DADLSB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.393"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JN331_004425"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JN331_004425"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY7262321.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          260 a          220 c          221 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcggaatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgaaaaa aaacctcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DADLSB010000387          981 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.394, whole genome shotgun sequence.
ACCESSION   DADLSB010000387 DADLSB010000000
VERSION     DADLSB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.394"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            194..981
                     /locus_tag="JN331_004426"
                     /pseudo
     CDS             194..981
                     /locus_tag="JN331_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          325 a          209 c          170 g          277 t
ORIGIN      
        1 ggttgaacta tatccctgct tttcaaccat cctaaaaaag catgaattgc tcgctctacc
       61 ctaagtggct gtgctatttt agaaaaactt aatcctgagt taagcaaaaa aacataatca
      121 ataaaaatac atacgcaaaa aacattgatt aagtgaatat accatggaag aaaaatataa
      181 accggagtag tgtatggcca atctctacga cttgaaaaag ttcgacttaa atcttcttgt
      241 catatttgag tgtatttacc agcatttaag cattagcaaa gctgctgaat cactatatat
      301 caccccatca gccgtgagcc agtcgttaca acgcttacgt gcgcaattta atgacccttt
      361 attcattcgc tcaggaaaag gtatcgcgcc gacgacaaca gggttgaatc tgcatcacca
      421 tcttgagaaa acctcaaagg ccttgaacaa acaattaata tagtcaataa gtccgaacta
      481 aagaaaaact ttattatcta cggccctcag ctcatttctt gctctaataa tagtatgctg
      541 atccgctgcc tacggcaaga tgcctctgtc gagattgagt gccatgatat cctcatgtca
      601 gcagagaatg ctgaagaact gttggttcac cgcaaagccg atctggtgat cacccaaatg
      661 ccagtgatca gccgttccgt catttgcatg ccattgcata caattcgcaa tactctaatt
      721 tgtagtaata agcatccgcg aattactgac aatagcactt atgaacaaat tatggcagaa
      781 gagttcaccc aattaatatc caaatcagca ggagttgatg attttcaaat ggaaatagac
      841 gagaagttta tgaatcgcaa aatttcattt cgtggttcct ctttgctgac aataattaat
      901 agcattgcag ttacagattt actgggaata gtcccctatg aactttataa ctcccaccgt
      961 gactttctta atttaaaaga g
//
LOCUS       DADLSB010000388          975 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.395, whole genome shotgun sequence.
ACCESSION   DADLSB010000388 DADLSB010000000
VERSION     DADLSB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.395"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(36..551)
                     /gene="sfmF"
                     /locus_tag="JN331_004427"
     CDS             complement(36..551)
                     /gene="sfmF"
                     /locus_tag="JN331_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HAY7262322.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(562..>975)
                     /locus_tag="JN331_004428"
     CDS             complement(562..>975)
                     /locus_tag="JN331_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7262323.1"
                     /translation="CELNAGQIVEFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVKC
                     TNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDAA
                     AAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
BASE COUNT          258 a          266 c          219 g          232 t
ORIGIN      
        1 atcgactacg ccaaattata cgaattaata taatattaat tataattgat cataaatatc
       61 gcatccgctt tcgccacacc tggccgaaca ccgctggcta acgctcgata attggcaaaa
      121 aagtttagtg tgacattgcc gtttgcatct acttcctcag tcgggctcgc ctcccccagt
      181 gcgagccggg agcgatcgct attacgtaat tcgatggcga cggtttgcgc cattgcggga
      241 tcatccagag ccagcaggtt ggtatcggat gccggcgttc ccgtaaataa aatcgcaact
      301 gaacccggag gacatccctc cagccgcagg ctaaaaggga cgagtgccgt ggtatcgcca
      361 gcgttcagta attgtgtcgt aggccatctg cctaaatcta ccgtcttatc aatatccgct
      421 gtgtttacgg tacaggagaa atcaacaacg ttaccgtgca aattgatatt aatcgttcct
      481 aaagggtcag ctgcccatcc actggaactc cacagtaacc cgcagaaaca gctaaagaat
      541 actcttctca ttctccgtac ctcattgata atcgacgcgt aaatacccca gcgcgctaaa
      601 cggcccttcg gtcggttttt gaccggtaat actgataggc caggcgcgaa gtgtgacatt
      661 ggctgccgca gctgcatcca gacggaaagg aataacgcta ttgagatcgt taggcgtgat
      721 cggcgtatcg ttctgatcgg cgacaataaa acctaaatcc tgattgtccg acaccatcgc
      781 ctgaccagaa acggcactgg cttccagacg cattgttaaa taagcctgcg cagcaacatt
      841 cgtacatttg accgcaatgc tcttggtttg cggcatgacg ccagctggtc gattccccgg
      901 ccctgccgca ctaaataacg atgcgccgat atcaccaaaa tcaaattcaa caatctgccc
      961 ggcatttaat tcgca
//
LOCUS       DADLSB010000389          965 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.396, whole genome shotgun sequence.
ACCESSION   DADLSB010000389 DADLSB010000000
VERSION     DADLSB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.396"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(1..483)
                     /locus_tag="JN331_004429"
     CDS             complement(1..483)
                     /locus_tag="JN331_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7262324.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="JN331_004430"
     CDS             complement(474..776)
                     /locus_tag="JN331_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7262325.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          293 a          236 c          195 g          241 t
ORIGIN      
        1 tcagacttta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctgggta caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgc
//
LOCUS       DADLSB010000390          964 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.397, whole genome shotgun sequence.
ACCESSION   DADLSB010000390 DADLSB010000000
VERSION     DADLSB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.397"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            83..>964
                     /locus_tag="JN331_004431"
     CDS             83..>964
                     /locus_tag="JN331_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HAY7262326.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     GNPKFRVIYQPVYSPWVN"
BASE COUNT          259 a          249 c          262 g          194 t
ORIGIN      
        1 agatcacttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc aaatcaaaac
       61 aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg acgaacgacg
      121 cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca gactgactgc
      181 catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc tctgctgcgc
      241 ccgctcctct gttggacgct ggattaactg gttcacgcag tcgggtgttg agggactgaa
      301 atcattacct gccgggcgtg cccgtcgctg gccgtttgag catatctgca cactgttacg
      361 tgagctggta aaacattctc ccggcgactt tggctaccag cgttcacgct ggagtacaga
      421 actgctggca ataaaaatca atgagataac cggttgccag ttaaatgccg gaaccgttcg
      481 ccgctggttg ccgtctgcgg ggattgtgtg gcgaagggct gcgccaactc tgcgtatccg
      541 tgacccgcat aaagatgaaa agatggcagc aatccataaa gcactggacg aatgcagcgc
      601 agaacatccg gtcttttatg aagatgaagt ggatatccat cttaatccca aaatcggtgc
      661 ggactggcaa ctgcgcggac agcaaaaacg ggtggtcacg ccgggacaga atgaaaaata
      721 ttatctggcc ggagcgctgc acagcgggac aggtaaagtc agctatgtgg gcggcaacag
      781 caaaagttcg gcgctgttca tcagcctgct gaagcggctt aaagcgacat accgtcgggc
      841 gaaaaccatc acgctgatcg tggacaacta cattatccac aaaagccggg aaacacagag
      901 ctggctgaag gggaacccga agttcagggt catttatcag ccggtttact cgccatgggt
      961 gaat
//
LOCUS       DADLSB010000391          956 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.398, whole genome shotgun sequence.
ACCESSION   DADLSB010000391 DADLSB010000000
VERSION     DADLSB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.398"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(304..747)
                     /locus_tag="JN331_004432"
     CDS             complement(304..747)
                     /locus_tag="JN331_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262327.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVAAVVRTALRAVRDTVVSVARAVWNWIFFLTR"
BASE COUNT          253 a          231 c          253 g          219 t
ORIGIN      
        1 ccggctcatc aaccatttgc cggagcagat tgatgctcag ttgcaaaagc cggtcggctt
       61 catcacaaag cagggtgatg ttcttttcgt gcattattta accttcctga gacgcatttt
      121 catgacaacc acggaggagg ccatttacta caaacgatcg tttatagcga tcaatataat
      181 agcaacgatc gttatattct attaatatga aaaacacaac gcaaggggga caacccgggt
      241 atggctgcgt ttgcattgtg ttttcataga gttaaaaatc tgtaacgaca acccggcaca
      301 tcactaccgg gttaagaaga aaatccagtt ccataccgcg cgggccacag agaccacggt
      361 gtcacgaacg gcgcgcaggg ccgtacggac aacagcggca acacagacac tctccgccgt
      421 gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg gtctgagtgc ggacagactc
      481 cgtgcgcagt tcgtcctgca ggcgtgtcat cagagggatg gcggcatgtc cgggaagcac
      541 tgtcatgagt gcactgaccg gggtgacctc cccctgaaac agtgcattac agagtgagga
      601 tttaccggcc ccgcttttac ccataatgcc aggcacgggt tcgtgacggg ggagtttgcg
      661 cagatgttcc aggatgtgac gggaaatcgg gtaaggcagg gaggagagcg gtttttcaat
      721 tgcctcaatg gcatcagacg gattcataca tgtattccca gtgaaaaaag acaaaacccc
      781 gtgcaccgga aaggtggcgg gggattcacg gagataatat ctttctgaaa ttattttgaa
      841 tgcaggtata tgtggctgat ttgtatcagc taatgagtac aggtctgaat ctaatctgac
      901 agtccgcttt gtgccaggag cggactgacc tgctccccga tgattaatac acagct
//
LOCUS       DADLSB010000392          926 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.399, whole genome shotgun sequence.
ACCESSION   DADLSB010000392 DADLSB010000000
VERSION     DADLSB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.399"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..97)
                     /locus_tag="JN331_004433"
     CDS             complement(<1..97)
                     /locus_tag="JN331_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262328.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNE"
     gene            236..907
                     /gene="queE"
                     /locus_tag="JN331_004434"
     CDS             236..907
                     /gene="queE"
                     /locus_tag="JN331_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY7262329.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          260 a          193 c          219 g          254 t
ORIGIN      
        1 attcgttatc attaaaaact aatacagaaa aaataaatat aattttggtg aatgttttga
       61 catgattaaa tccattttct ttattttctt ctgacataat acgttactcc ctttaattat
      121 taacaatatg tttcgtattt ttctttaaat ctgcatgtaa cagaatgtta ataagacatg
      181 attgttgcgg caggatctgc aataattaac aattatccac ttcacagaga atgctatgca
      241 gtacccgatt aacgagatgt tccagaccct gcaaggtgag ggttacttta ccggcgtccc
      301 cgccattttt attcgtttac agggatgccc ggttggctgt gcctggtgcg acaccaaaca
      361 cacctgggaa aagcttgagg atcgggaagt ctcccttttc agcattctgg cgaagaccaa
      421 agagagtgat aagtgggggg cggcgagcag tgaagacttg ctggctgtca ttggtcgtca
      481 gggatatacc gcgcggcatg tggtgattac gggtggtgag ccttgcattc atgatttgct
      541 gccactgact gacctgctcg aaaagaacgg ttttagctgc cagatcgaaa ccagtggaac
      601 tcatgaagta cgctgcacac cgaatacctg ggttaccgta tcgccaaagc tgaacatgcg
      661 cggcggctat gaagtgttgt cacaggcact ggagcgagcc aacgaaatca agcatccagt
      721 ggggcgcgta cgcgatattg aagcactgga tgaactactg gcaacgttga ccgatgataa
      781 accgcgagtc attgcactgc agccgattag ccaaaaggat gatgccacac gattgtgcat
      841 tgaaacctgt attgcgcgta attggcgttt gtcgatgcaa acacataaat atttaaatat
      901 tgcctgatta cacatttata agcgtt
//
LOCUS       DADLSB010000393          912 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.400, whole genome shotgun sequence.
ACCESSION   DADLSB010000393 DADLSB010000000
VERSION     DADLSB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.400"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            28..>912
                     /locus_tag="JN331_004435"
     CDS             28..>912
                     /locus_tag="JN331_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262330.1"
                     /translation="MSENKSFSTAVPAVQISDSGLNVPDEADILSGRLADFSAALGGA
                     MSTSLSSPQGQLASSESAIIADKNDQLLYIVNQINPDFSSGRFQDAIGRIYFLERRGA
                     TGTTVTATCTGLVGTLIPAGSMVQDEAGYKYVSQSDVTIGASGLVDAVFLNLSTGPVG
                     CPVGTLNKIYKAIPGWSGVTNASAGVPGSDEETRADFENRRRNSVARNARNILEAIRG
                     EILSTVENVVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAP
                     PGIDMSGNTTFTVADKEYD"
BASE COUNT          218 a          216 c          265 g          213 t
ORIGIN      
        1 caataatgac atttttacgg tactgctatg agtgaaaata aatctttttc taccgcagtg
       61 cccgctgtac agatttcgga tagcgggctg aatgtgccgg atgaagcgga tattctgagc
      121 ggcaggcttg ctgatttttc ggcggcgctg ggcggggcca tgagtaccag tctgagcagt
      181 ccgcagggac aacttgcatc cagcgaaagc gccattattg cggataaaaa cgatcagttg
      241 ctgtatatcg ttaaccagat caaccctgac ttttccagtg gtcgctttca ggatgcaata
      301 ggcaggattt atttcctgga gcgccgcggg gccacaggta cgacagtaac agcgacctgt
      361 accgggctgg ttggcacgct gatcccggcg ggcagtatgg tgcaggatga ggccggttat
      421 aagtatgtca gtcagtcaga cgtcactatc ggcgcatcag ggctggttga tgcggtattt
      481 ctgaatttgt ccaccggccc cgtcggctgt cctgtgggga cgctgaataa aatttataag
      541 gcaatacctg gctggtcagg tgtcactaac gccagtgccg gtgtgccggg cagcgatgag
      601 gaaacccgtg cggactttga aaaccgccga cgtaattcag ttgcccgtaa tgcccggaat
      661 attctggaag ccatccgggg tgaaatactc tccacggtag aaaacgtggt ggatgtttac
      721 gtcacccata atccgaaaaa aacagaacaa aaagccggag tcagtcagta tccattaaca
      781 cccggttcgc tttatgttgg cgtgtacggc ggcagcccgg cagatatcgc ggcggccatt
      841 tggcgtaagg ctccgccggg tattgatatg agtggcaaca caacgttcac tgttgcagat
      901 aaggagtacg at
//
LOCUS       DADLSB010000394          910 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.401, whole genome shotgun sequence.
ACCESSION   DADLSB010000394 DADLSB010000000
VERSION     DADLSB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.401"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            157..821
                     /locus_tag="JN331_004436"
                     /pseudo
     CDS             157..821
                     /locus_tag="JN331_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
BASE COUNT          287 a          192 c          199 g          232 t
ORIGIN      
        1 aaagcttatt ttcagcttta attaactaac cacgtcattg ttctgctgct aataacaaca
       61 aaggtgggtg gtttatgatt attcacttta atacaccagg tgaactcctt ctgccatgca
      121 ggcagggttg gacagaaaac acgcgaagga attaaaatgc ctggaacggg aaaaatgaaa
      181 catgtcagtt tgactctgca ggttgagaac gacctgaaac atcagcttag tattggcgca
      241 ttaaaacctg gcgcacgcct gattactaaa aatctggcgg agcaattagg tatgagtatt
      301 acacctgtgc gtgaagcatt attacgtctg gtttcggtga atgcgctttc tgtcgcacct
      361 gcacaagcat ttacagtacc ggaagtgagg caaagtcagt tagacgaaat caatcggatc
      421 cgctacgaac tggaattaat ggcagttgcc ctggctgttg aaaacctctc ctcgcaagac
      481 atagcagaac ttcaggaatt gctcgggaaa ttacagcagg cgcaagaaaa gggcgacatg
      541 gaacaaatca ttaatgcgaa caggctattt cgcttagcga tttatcatcg ctcaaatatg
      601 cccatcctgt gtgagatgat tgagcaactg tggatcagga tgggacctgc tttacattat
      661 ctttatgaag cgattaaccc agcgtaatta cgggagcgca tagaaaacta tcatctatta
      721 ctcgctgcgt taaaagcaaa agacaaagag ggatgcaaac attgtcttgc tgaaattatg
      781 caacaaatat tgctatttta taccagcaat acaatcgtta aattaaatgc ccggcaaata
      841 ccgggcatca agtaattact caaatcgcca cgcaatattc atgccgacac cataatttcg
      901 tccgggcgca
//
LOCUS       DADLSB010000395          906 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.402, whole genome shotgun sequence.
ACCESSION   DADLSB010000395 DADLSB010000000
VERSION     DADLSB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.402"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            62..>906
                     /locus_tag="JN331_004437"
     CDS             62..>906
                     /locus_tag="JN331_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7262331.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATVALINGASLTLTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIK"
BASE COUNT          269 a          190 c          207 g          240 t
ORIGIN      
        1 agacagacat agcattatgt gatctactga tttgtgccaa caattcaatg agatcactgc
       61 tatgcctgtt cataaagtat gtcagaattt cttccgggat gccttagcac catttcatca
      121 gtaccgacaa aatgccctga tggatgccac agtggcgtta atcaacggag catctctgac
      181 gctgaccagt atcggacgtt ttttgccggg taatgcacaa gttaagaata agatcaaacg
      241 tattgatcgc ctcatgggta atgaggcgct ccatcgcgat attccaatga ttttcaggaa
      301 tatcacttca atgctgacac ggcagttatc tttatgtgta atagccgtag actggagtgg
      361 ctatccctcc caggagcatc atgttcttcg tgccagcctt ctgtgtgacg ggcgttccat
      421 tccgttattg agtaaggttg ttccttctga aaaacaaaat aatccattga tacaacatga
      481 tttccttgat tcccttgctc aatccctgcc ccccgatgcc agggttatta ttgtcacgga
      541 tgctggattt cagagcgcat ggtttcacca tattacttct ctgggctggg attttattgg
      601 tcggatcaga aataacgttc agtattgcct cgataacgcg cctgaaagat ggttaaaagt
      661 gtcggatagc cctgaatgta aaacaccgga atatatgggg gctggaaggc tggttaaaga
      721 aaggaaaaaa agcatccggg ggcattttta cacctataag aaatcggcaa aagggcgaaa
      781 aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca gacaaagaac agagcaaatc
      841 agcaaaagaa gcctggttga tcttcagcag tacaaatgac ttcagagcgc gagaaatcat
      901 caagtt
//
LOCUS       DADLSB010000396          874 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.403, whole genome shotgun sequence.
ACCESSION   DADLSB010000396 DADLSB010000000
VERSION     DADLSB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.403"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..226)
                     /locus_tag="JN331_004438"
     CDS             complement(<1..226)
                     /locus_tag="JN331_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262332.1"
                     /translation="MKWLKLIFWGLCGLQICQLSYAQEALTEKYSQSELLRNWALSHC
                     LSLVYKNDAVQDDARATASAYLEYGQQPVEI"
     gene            complement(223..709)
                     /locus_tag="JN331_004439"
                     /pseudo
     CDS             complement(223..709)
                     /locus_tag="JN331_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(762..>874)
                     /locus_tag="JN331_004440"
     CDS             complement(762..>874)
                     /locus_tag="JN331_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262333.1"
                     /translation="GYYDAKFFLRPHFQLEGLTGSLRLVTKLPSVKQGNA"
BASE COUNT          281 a          208 c          144 g          241 t
ORIGIN      
        1 aaatttctac aggttgctga ccatattcaa gataagcact agctgtagct ctggcatcat
       61 cctgaacggc atcattttta tacaccaatg acaaacaatg actcaaagcc caattgcgta
      121 agagctctga ctgagaatat ttttcagtca atgcttcctg agcataagat aattgacata
      181 tctgtagccc acagaggccc caaaatatta atttcagcca tttcatggta gtacccacag
      241 gattccgact tcaggaacaa atgttccatt atctggatca tttagtagat gacattgatc
      301 tgaacaaacg ctaccatccc ataatgttac atgtccccga gcattgctcc agccatggcc
      361 ttttacaaca atgatgcctt ttttacctgt aaagtcacca ggagttgtga ttattaatga
      421 caagatctgg ctcccccata atctgggtca gatgttcaat catatcattc acacgataaa
      481 tataaaattt cttatcagaa ccactaaccg ttgcataggg agaactacgg gatatcggaa
      541 atcccgtagc attcaaaaca taactcatac gaattggaca agcattttca aaatagccac
      601 ctgaaatatt ttgcccaaca tttccgccaa ttatacgacc gacatcagca acgggaaaat
      661 tcacttttcc aaatgccgac catgcctcat taaatgaagg tctacccatt ttctttaccc
      721 tctaaaaaat ctgcatcaca agctttatga aatataataa atcaggcatt accctgtttt
      781 accgacggca gttttgtgac cagacgcagt gatcctgtta acccttcaag ctgaaaatgc
      841 gggcgcagga agaatttagc atcgtaataa cccg
//
LOCUS       DADLSB010000397          861 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.404, whole genome shotgun sequence.
ACCESSION   DADLSB010000397 DADLSB010000000
VERSION     DADLSB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.404"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            257..346
                     /locus_tag="JN331_004441"
                     /pseudo
     CDS             257..346
                     /locus_tag="JN331_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            370..>861
                     /locus_tag="JN331_004442"
     CDS             370..>861
                     /locus_tag="JN331_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY7262334.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFR"
BASE COUNT          213 a          200 c          202 g          246 t
ORIGIN      
        1 gcatcatttc tctgcgctta tcgagatact gagcatggtt gtaaattcag ctaacccagt
       61 gcgttgacaa aaaattagcg caagaagaca aaaaatcacc ttgcgctaat gctctgtatc
      121 aggtcactaa taccagctaa gtagttgatt catagtgact gcatatgttg tgctttgtcg
      181 cattttgtag tctattattt aatctgtaac aacgataata caatgaattt taattggttt
      241 ttgtgatttt gtccttcttc tgacatggaa ggacacccga tggataccca acgattccaa
      301 tcccaatttc attggcattt atcgtttaaa ttttctggcg cgatagcagc gtgcttgtcc
      361 ctgtctcttg tgggaacagg gcttgctaaa gctgatgatt cgcttccttc cagtaactat
      421 gcgccgcccg ccgggggaac attctttttg cttgctgaca gcagttttag cagcagtgaa
      481 gaggcgaaag tgcgactgga agcgccgggg cgtgattatc ggcgctatca gatggaagag
      541 tacggcggcg tggacgttcg cctgtatcgt attcctgacc cgatggcatt tttgcgccag
      601 cagaaaaacc tgcatcgcat tgtggtgcaa ccgcaatatc tgggcgacgg gctgaacaat
      661 acgctgacct ggctgtggga taactggtac ggcaaatctc gccgcgtgat gcagcgtact
      721 ttctcttctc agtcacggca gaatgtgact caggcattac ccgaattaca tctcggcaat
      781 gccattatta aaccttcccg ttatgtacag aacaaccagt tttccccgct gaaaaaatat
      841 cccttggtgg aacagttccg t
//
LOCUS       DADLSB010000398          842 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.405, whole genome shotgun sequence.
ACCESSION   DADLSB010000398 DADLSB010000000
VERSION     DADLSB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.405"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(94..459)
                     /locus_tag="JN331_004443"
                     /pseudo
     CDS             complement(94..459)
                     /locus_tag="JN331_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(732..>842)
                     /locus_tag="JN331_004444"
     CDS             complement(732..>842)
                     /locus_tag="JN331_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7262335.1"
                     /translation="FRTCCSRVDVWLNLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT          221 a          201 c          216 g          204 t
ORIGIN      
        1 atcatcatcc cgtaactgaa ttgtggaaac agtgacaccg cctctctgat aacagagaac
       61 tcgcgtgtcg taacttttct ggcatgaaac tggcgttgac ggatcctttt gactgaaata
      121 gcagtcttcc agtttttcga acacatccca cgcctgatcg gtttcgagca ttttggcgtg
      181 gcgtgctgcg cctcgttctg tccagaggat gagggagcgg gctttgggtg aaactggatt
      241 ttgtgagtag tttaaagcga cccgcaattc tttaaggtca ttaccaacaa ctttgaaaaa
      301 gtgtttccct tcaacgaagc gtactttgtt ctcatgatga ttctggcgaa tacgcaccgg
      361 ctcagtgccg taaagctgcg ccaaaagttc ggtggtaata acaggaatct ggttatgggt
      421 gatcggggag agagtttcaa cagagatttg agtggtcata acgataactc cgtacatttg
      481 gaaattatcg ccaccgacga cgccaatcga actggtggcg aactgtgcag ggttggcgta
      541 accgggtacg gaaaccggcg agcctttcgg ctcccccaca cagcccgcca taaatcgcga
      601 atgtgactat gcaaacgata tgaaaaaaga cgcgggcgcg tctcatatcg ctccgtaaac
      661 atccggggtg ctaatcccga cgccagattt tgctggcgcg tgaggaatat agccccggat
      721 aacagattga gtcaacaaac ggtttttaga tccccggaag agaatgcatc acgcatcggt
      781 agatagagca tatattcagc aagatttagc catacatcta cccgactgca gcacgtccgg
      841 aa
//
LOCUS       DADLSB010000399          823 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.406, whole genome shotgun sequence.
ACCESSION   DADLSB010000399 DADLSB010000000
VERSION     DADLSB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.406"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            29..>823
                     /gene="ltrA"
                     /locus_tag="JN331_004445"
     CDS             29..>823
                     /gene="ltrA"
                     /locus_tag="JN331_004445"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7262336.1"
                     /translation="MKDRNGSGAKGLPHCADGAAATTGDNADGRTAVKSAKPFPVSKR
                     QVWEAYKRVKANRGAAGIDGQTLAGFDENVTDNLYKLWNRMASGSYMPQAVRRVDIPK
                     ADGGVRPLGIPAVSDRIAQMVVKQILEPVLEPLFHADSYGYRPGKSAHQAIAQARKRC
                     WKFDWVVEVDIKGFFDDIDHHLLLKTVQHHTQARWVVMYIERRLKAPVQMPDGAMLAR
                     GRGTPQGGVISPLLSNLFLHYAFDMWMQRQFPGVPFERYADDVVCHC"
BASE COUNT          195 a          197 c          256 g          175 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc tttgatgata tcgatcatca cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tgt
//
LOCUS       DADLSB010000400          818 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.407, whole genome shotgun sequence.
ACCESSION   DADLSB010000400 DADLSB010000000
VERSION     DADLSB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.407"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..152
                     /locus_tag="JN331_004446"
     CDS             <1..152
                     /locus_tag="JN331_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262337.1"
                     /translation="WFLQLLDRVSPSQWAAIGVLGSLLFGLLTYLTNLYFKIREDRRK
                     AARGE"
     gene            157..501
                     /locus_tag="JN331_004447"
     CDS             157..501
                     /locus_tag="JN331_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HAY7262338.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARMSIRQVFLDVAAGLCEGDEQSPEKRPIILEAQNVWITYKGKLPG
                     RITDSLKTPPKW"
     gene            complement(467..739)
                     /locus_tag="JN331_004448"
     CDS             complement(467..739)
                     /locus_tag="JN331_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262339.1"
                     /translation="MDNNTISLQELLDCISRLRDDVNALTVAFSSLALSIPREQMQPT
                     LASLLLESRNPKWSQEQQNSFKWLAALLEEKYAGKITISVESSENQ"
BASE COUNT          217 a          164 c          220 g          217 t
ORIGIN      
        1 actggttcct gcagttgctg gacagggttt ccccgtctca gtgggcggca ataggcgtgc
       61 tggggagtct gctgtttggg ctgctgacat atctgactaa cctgtatttc aaaatcagag
      121 aggaccgtcg taaggctgca cggggagagt aattcaatga ctcaaaacta tgaactgatt
      181 gtgaaaggga tccgcaattt tgagaataaa gttacggtaa ctttagcgtt acgggacaaa
      241 aaacgctttg acggtgaaat ttttgacctg gacatctcgc tggaccgtgt tgaaggtgcc
      301 gcgctggagt tttatgaggc agcagccaga atgagcatca gacaggtctt cctggatgtt
      361 gctgccgggt tatgtgaagg ggatgagcag tcgccggaaa agcgccccat aattttagag
      421 gcgcagaatg tgtggataac ctacaaagga aagctaccag gaagaattac tgattctctg
      481 aagactccac cgaaatggta attttaccag catatttttc ttccagtaat gccgccagcc
      541 acttgaaaga attttgttgt tcctgggacc atttggggtt gcgtgattca agcaggagcg
      601 atgccagtgt tggttgcatt tgttctctgg gaattgataa ggccagagat gaaaatgcaa
      661 cagtgagggc atttacatca tcccgaagcc tggaaatgca gtcgagcaac tcctgtagag
      721 aaatggtgtt attgtccata aataatcctc ttgattgtct ttaccttttc ccagcctgat
      781 tcaacaggct gggacagata aacatatcca gggttcag
//
LOCUS       DADLSB010000401          814 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.408, whole genome shotgun sequence.
ACCESSION   DADLSB010000401 DADLSB010000000
VERSION     DADLSB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.408"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..251)
                     /locus_tag="JN331_004449"
     CDS             complement(<1..251)
                     /locus_tag="JN331_004449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262340.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIA"
BASE COUNT          214 a          206 c          163 g          231 t
ORIGIN      
        1 gcaatttccg gatacccttc gtcatcaaca cgtacaaacc agaagaccag ttttttgttt
       61 ccggcatcca caaagaacgg aatattcagg tctgcgcaac actcaacggc atcgtcaaaa
      121 ttatcaaagc gcagaacctc agcttcttct tcgtcaaaaa aatcatcttc gtgaagtttt
      181 acgacatagc ggaggagttt gcttctttga ggtgcaggtt ttcgtttgcg tggtttagct
      241 gaacgggcca tataactacc tgaaagacaa tgatattgtc tgtttttata acggtaatca
      301 caggccatga caagccgcag ccgtcaggcc gcctactcgg ggcggtttag aacgcttatt
      361 tagtaccaga tcttacttat aataaagggg cttggcagtt tggataaact attgtcctta
      421 ttccactaat acaaactttt tgtaaagtat attaaatata cttcatttca tactgaaatc
      481 cccggaagta ttcaatcaaa caattacacc ataaaaggaa gaggggcccc aattaagttg
      541 tcaagcatgt ttactgcgca atcgcttggt gctccgtccc tgtaccccgc attggtattc
      601 tggcgctcat ggggcttcca cttgcgcttc tcccttcaca tcggggtgac aggtccacgt
      661 tccccacaag agcctgaatc gacttcacgc cgcttttatg ccggatgccg cgcaggcagt
      721 aaacatatcc cacaggaacg actccaccat tgttttgaca tcatctatat gctttcgaca
      781 catcatcagc ggttcacttt cgttcgtctc ttcg
//
LOCUS       DADLSB010000402          797 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.409, whole genome shotgun sequence.
ACCESSION   DADLSB010000402 DADLSB010000000
VERSION     DADLSB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.409"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>797
                     /gene="narH"
                     /locus_tag="JN331_004450"
     CDS             <1..>797
                     /gene="narH"
                     /locus_tag="JN331_004450"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7262341.1"
                     /translation="KNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDV
                     NGKIRPRLGSKMGVITKIFANPVVPQQIDDYYEPFTFDYEHLHSSPEGKHIPTARPRS
                     LIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLPRLCEH
                     CLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKSEKC
                     IFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDL"
BASE COUNT          211 a          206 c          225 g          155 t
ORIGIN      
        1 aaaaacgtct ggaccggacg cgaaggcatg gagtacgcat ggtttaacaa cgtcgaaacc
       61 aaaccgggca ttggttatcc gaaaaactgg gaagatcagg aagagtggca aggcggctgg
      121 gtccgcgatg tgaatggcaa gatccgtccg cgtctgggca gcaagatggg cgtaataacc
      181 aaaatcttcg ccaacccggt ggtgccgcag cagattgatg attactacga acctttcacc
      241 ttcgactacg aacatttgca tagctcaccg gaaggcaaac atatccctac tgctcgcccg
      301 cgttcactga ttgacggcaa acggatggac aaagtgatct ggggcccaaa ctgggaagaa
      361 ctgctgggcg gcgagtttga aaaacgtgcc cgcgaccgca acttcgaggc catgcaaaag
      421 gagatgtacg ggcagtttga aaacaccttc atgatgtacc tgccgcgcct gtgcgaacac
      481 tgcctcaatc ccagctgcgt ggcgacctgc ccaagcggcg ctatctacaa acgcgaagaa
      541 gacggcattg tgctgattga tcaggataaa tgccgtggct ggcgtttgtg cattagcggt
      601 tgtccgtaca aaaaaatcta cttcaactgg aaaagcggca agtcagaaaa atgtatcttc
      661 tgttatccgc gaattgagtc aggccaaccc accgtgtgct cagaaacctg cgtgggtcgc
      721 atccggtatc tgggcgtgct gctttacgac gccgaccgca tcgaggaagc ggcgagcacc
      781 gagcgcgaag ttgacct
//
LOCUS       DADLSB010000403          783 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.410, whole genome shotgun sequence.
ACCESSION   DADLSB010000403 DADLSB010000000
VERSION     DADLSB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.410"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(149..661)
                     /locus_tag="JN331_004451"
     CDS             complement(149..661)
                     /locus_tag="JN331_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7262342.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
BASE COUNT          237 a          162 c          177 g          207 t
ORIGIN      
        1 gaccagacag cattgagaat gcttcaggag tacatcagga tccaatgcct tggggggtag
       61 cggtaatttt actcattatc cttcaaatca gattctgttg tcagaggaat gggggaagct
      121 ggattcgcaa gagcttttta tgggcagttt agctaaaaaa aggcaacacg aagctaaaac
      181 taccaacata aagtgctgac cagctagcag ctgaaatcag actcactgta tacactttca
      241 ctggatgaag ttgcaacatt cctgccacca aaggagcaat gtaacgcagc acggcgataa
      301 agcgcgaagt gaaaagtatt gacacagagt tattttgcaa ctgaaggcga acccgttcta
      361 ttgtattgga acggctcgat attatacatg caagttgagg gatatgccca attattatac
      421 caagatggta attaacgata gtaccgcacc atgcgccact catgaccgtg attccagctt
      481 cccatggtga aagagtcgtc ttgctgactg taattacagt ggccatcata attgatgctg
      541 gtggcaatat cgccgaaatg agtaaagtgg attttgtgaa cgctattata aaaagaagcc
      601 cccataggcg atatggatga agtacaaaat agttcatcaa agcatttatc cactccatca
      661 atgtctttcc ttctgtgaat ttttctttgc tatttaaccg tgatacgact taatttaggc
      721 ttaaacacct tacatttaca aggtactcct gtggggggcc ctgccacgga gcgtcgcagg
      781 agt
//
LOCUS       DADLSB010000404          778 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.411, whole genome shotgun sequence.
ACCESSION   DADLSB010000404 DADLSB010000000
VERSION     DADLSB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.411"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..310
                     /locus_tag="JN331_004452"
                     /pseudo
     CDS             1..310
                     /locus_tag="JN331_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            505..744
                     /locus_tag="JN331_004453"
     CDS             505..744
                     /locus_tag="JN331_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7262343.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
BASE COUNT          189 a          205 c          222 g          162 t
ORIGIN      
        1 cagcggcgga caaggataaa cacacgtcag cagttccgtc agcactgcga cagcgttgtc
       61 cgcaaggctt ttacccagtc cagcgttacg gtgccccacg cctgacggat gaactgtgtg
      121 ctctggttac cactttaacg taaaaaccgt ggcagccagt ctgcgccgtc aggaactgag
      181 cgcaaaagcc tcccagaagt tcagcccgat cagttaccgt gcacacggcc tgcctgtatc
      241 agaaaatctg ctgacgcagg atttttacgc cagtggcccg aaccagaagt gggcgggaga
      301 catcacgtac tattacagct caccgactgc ggggaaacac ggggcgccgg gttattgaag
      361 gtgacctgtc cagctacttc gacacagtac atcaccggct gctgatgaaa gccgtctgcc
      421 gcaggatcag tgatgcacgt tttatgagac tgctgtggaa aacgccgtgt tgatgttggt
      481 ctcttccggg cggtcagtga aagtatgcca cagggcggtg tgatctcgcc gttgctgtcg
      541 aacatcatac tgaatgagtt cgaccagtat ctgaataaac gctacctgag tggaaaagcc
      601 agaaaggatc gctggtactg gaatcacagt atccagcgag gccgaagtac tgctgtcaaa
      661 gaaaactggc aatggaaacc tgccgttgct tactgttgct atgccgatga ctgtgtgcca
      721 cgaaggcgcg tcctggggac gtgagtccct gtgcagccat gctgcacaag agatgagg
//
LOCUS       DADLSB010000405          775 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.412, whole genome shotgun sequence.
ACCESSION   DADLSB010000405 DADLSB010000000
VERSION     DADLSB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.412"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(247..729)
                     /gene="sseA"
                     /locus_tag="JN331_004454"
                     /pseudo
     CDS             complement(247..729)
                     /gene="sseA"
                     /locus_tag="JN331_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT          194 a          211 c          171 g          199 t
ORIGIN      
        1 ttcggactaa taagccaggt tattttatta ccgaacacgg cggaaagggc cggtgtcgat
       61 aatacgctaa ataaacaata attactctct tttgcttgac aaaaagagag ttactggtga
      121 gtattgtttt gctgtgaagc acctgcgttg ctcatgtaac acaagtcaac ctgctggaaa
      181 ggccgcgaac cagaccagca ataaaaaaac gccaaattcg gcggtttttt attgcctgta
      241 aaatacttat ttcactggct caaccggtaa atctgcccgc gcgccccatt cactccatgc
      301 gccgtcgtac agtttcacgt ttggcacatc cagcgtcgcg agtgctaaca aaaccacggc
      361 tgccgttaca ccagagccgc agctgacgat aatcggtttg tcatagctga cgccgcgacc
      421 aaaaaatatc gcatccagtt catcggtcgt ttttagttcg ccttcgcgca ccagttccgt
      481 ccacggaacg ttcaatgcac cgggaatatg tccgcgacgt aaacctgggc gaggttcatc
      541 aacttctgcg ttaaaacgtg cagccgggcg ggcatcaata atttgcggcg tattttcatg
      601 gcttgccaat aacacatcgg ttactttcac cactgcttca ggattaaacg cggcgttaaa
      661 ctctccttcc ggcagctcta ctgcaccttc ttctaatagc aggtcatcgc gctgccagcc
      721 tgcaagtccc cgttacttta ttcgtacccc ttataatggg gtgttagcca gccag
//
LOCUS       DADLSB010000406          763 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.413, whole genome shotgun sequence.
ACCESSION   DADLSB010000406 DADLSB010000000
VERSION     DADLSB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.413"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>763
                     /locus_tag="JN331_004455"
     CDS             <1..>763
                     /locus_tag="JN331_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7262344.1"
                     /translation="YLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLK
                     GIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIK
                     MADGTLNACTAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMVEVNLYKVIAPYVNLFK
                     ADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNM
                     ANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFD"
BASE COUNT          253 a          133 c          120 g          257 t
ORIGIN      
        1 ttaccttagt aaagaaaagg atgtatccat taaggtggta tattttataa ataatgtcgc
       61 cgtccataat aatactatcg aaattccaca gatagtaaat ggtggttacg atttttcaca
      121 ccttagcctg aagggtatcg tgattaaaga tgaagattta tccaattcga attttgcagg
      181 ttgcagacta caaaacgcta ttttccagga ctgtaatatg tataaaacga atttttatta
      241 tgccataatg gaaaaaatac tttttgataa ttgtattctc gatgactcaa atttcgctca
      301 gataaaaatg gccgacggaa ctctaaatgc atgcaccgct atgcatgttc aattctacaa
      361 tgcagcaatg aatagagcca atattaaaaa taccttcctt gactattcaa atttttatat
      421 ggcgtacatg gttgaggtaa atctttataa agtaatagcg ccatatgtta atttatttaa
      481 agccgacctt agtttctcta aactcgattt aattaacttt gaacatgctg atctgtctcg
      541 cgtcaatctg aacaaagcaa tcctccagaa tataaactta attgatagca aactcttttg
      601 tacgtggctg acaaatacat tcctcgaaat ggttatatgt accggctcta atatggctaa
      661 tgttaatttt aataatgcca atttaagcaa ctgccatttc aactgttcta ttttaacaaa
      721 agcctgtatg tttaataccc gtctctatcg ggttaatttc gat
//
LOCUS       DADLSB010000407          755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.414, whole genome shotgun sequence.
ACCESSION   DADLSB010000407 DADLSB010000000
VERSION     DADLSB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.414"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..491)
                     /locus_tag="JN331_004456"
     CDS             complement(<1..491)
                     /locus_tag="JN331_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY7262345.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKH"
     gene            complement(638..714)
                     /locus_tag="JN331_004457"
     tRNA            complement(638..714)
                     /locus_tag="JN331_004457"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(678..680),aa:Pro,seq:tgg)
BASE COUNT          186 a          192 c          188 g          189 t
ORIGIN      
        1 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
       61 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
      121 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
      181 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
      241 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
      301 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
      361 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
      421 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
      481 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
      541 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
      601 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
      661 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
      721 tgctttttga atttttagtt caattcttta aagtc
//
LOCUS       DADLSB010000408          752 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.415, whole genome shotgun sequence.
ACCESSION   DADLSB010000408 DADLSB010000000
VERSION     DADLSB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.415"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..128
                     /locus_tag="JN331_004458"
     CDS             <1..128
                     /locus_tag="JN331_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY7262346.1"
                     /translation="LTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVFSIMGS"
     gene            152..>752
                     /locus_tag="JN331_004459"
     CDS             152..>752
                     /locus_tag="JN331_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7262347.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVILSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAF"
BASE COUNT          204 a          149 c          187 g          212 t
ORIGIN      
        1 ttcttaccgg ggccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc
       61 ttggtgttac cggccactcc gccctgaata tgtggtccgg tattgtcttc agtataatgg
      121 gttcctaaac acatcatttt aggtattgaa tatgagacgg gcaatcagct acatcagatt
      181 ttcaagtgaa cgacaactga aaggggactc tgtacgccga caatctaagc tggttactga
      241 ctggcttgat aagaaccctg agttttactt ggattcatcc ttgagcttta aagatttggg
      301 gaaaagtgct tttagtggta agcacctgaa aggtggtcta ggtgactttt taaccgctat
      361 cgagaaaggg cttgttaaag ctggtgatac gctactaatc gagagtttag accgtctgtc
      421 acgtcaggat attgatattg cttctgagct attacgccgt atcttaaggg ctggtgtaga
      481 tgtagtcata ctgagcgatg gagaacatta cacacgagaa agtttaaaag acccgttagc
      541 cctgattaaa agcattctga taatgcagcg ggcgcatgag gaaagtttga ggaagtctga
      601 gcgtgtccaa gctgcatgga accgtaaaaa agaactcatc tccgaaggta tcaaggtatc
      661 taggcgttgt cctgcatggt taaggctgaa tgatgaccgt cgaacattca caatcattcc
      721 tgataaagtg gaagttgtta aacgagcatt tg
//
LOCUS       DADLSB010000409          749 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.416, whole genome shotgun sequence.
ACCESSION   DADLSB010000409 DADLSB010000000
VERSION     DADLSB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.416"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..342
                     /locus_tag="JN331_004460"
     CDS             <1..342
                     /locus_tag="JN331_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7262348.1"
                     /translation="AKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRR
                     GIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIAR
                     VRSVEEILHAL"
BASE COUNT          218 a          176 c          167 g          188 t
ORIGIN      
        1 gctaaagtgt tgcccgaaaa ctggtggcaa catccagctg cattaggtgc aactgacagt
       61 gatatcgaaa tcatcaaacg tcagtggggt gcgttttacg gtacggatct ggagttgcaa
      121 ctgcgccgcc gaggtatcga tacaatagtg ttatgtggga tctcgaccaa tatcggtgtt
      181 gaatccaccg cccgcaatgc ctgggaactt ggctttaatc tggtgattgc cgaagacgcc
      241 tgtagtgccg ccagcgccga gcaacacaat aacagcatta atcatatcta cccgcgcatc
      301 gcccgtgtgc gtagcgtgga agagatcctt cacgcgttat gatttacatc ggtctaccgc
      361 aatggtcgca tcctaaatgg gtgcggttgg ggatcaccag ccttgaagag tatgcccgcc
      421 actttaactg cgtgatacgg gatttttaaa aatcactaaa gaacgcccaa gagcatgtgt
      481 tttctttaga ataatcaaag cgttggattg ccatgtcata tgaaaatcgg caaagttcat
      541 atgtaacaaa gtgtcccaat catgccccaa aatgccccac aacaaatatt tttgccccat
      601 gcatgcccca aaaaatcact ttccctcact cttgacactg tttatccata cagttaaaaa
      661 taatactgta taaaaacaca gtgtagaggg gtttttatgc gtattgaaat ctgcatagcc
      721 aaagagaaaa tgactaaaat gccaaccgg
//
LOCUS       DADLSB010000410          747 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.417, whole genome shotgun sequence.
ACCESSION   DADLSB010000410 DADLSB010000000
VERSION     DADLSB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.417"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..538)
                     /gene="rclC"
                     /locus_tag="JN331_004461"
     CDS             complement(<1..538)
                     /gene="rclC"
                     /locus_tag="JN331_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HAY7262349.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAR"
     gene            complement(550..741)
                     /locus_tag="JN331_004462"
     CDS             complement(550..741)
                     /locus_tag="JN331_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7262350.1"
                     /translation="MAFSTNADDKIILKHISVSSVSASPTVLEDAIADIARKYNASSW
                     KVTSMRIDNNSTATAVLYK"
BASE COUNT          214 a          176 c          155 g          202 t
ORIGIN      
        1 cccgcgccga atctgccatt atcatgacag cacctgccag catcagagta tctttcaata
       61 ccaaacgacc agcaccggat aaataaggga aaccatgatg agcgtcaccc aatgcgggta
      121 cccatgcctc cggggtggtg attaaaaatg agagtgttac cagcggcgtg gtaaatgcca
      181 tcagtcctcc caataaacct aaccagcgat tgacaggatt agccaaaacc agcagagcaa
      241 taatcacctc cacgacgcca agaccgttgg aaaaaccata ggtattattg gccgtttgcc
      301 atgcccttgc ttctggtttg tattcgcctt cgtgagtcag atactgttta tagtcttccg
      361 ggtgttcata aaagaacgac attagtggac tgtttgcgac gaatggtgta atgctgtctg
      421 cctcgtaagg gacaaacttt aataacccaa tccacataaa aacaattgca atactcagac
      481 gaatcaatgt caggccaatt ttatctcccc gacttaacag gtgcaggtat ttttccattg
      541 tgaacatcct tatttataca atactgctgt tgcggttgaa ttattatcaa ttcgcatcga
      601 tgtgactttc caggatgaag cattatattt tctggctata tcagcaatgg catcctccag
      661 aactgtcggt gatgctgata ctgacgagac gctgatatgt ttcagaatta ttttatcatc
      721 tgcattggtg gaaaatgcca taacgcc
//
LOCUS       DADLSB010000411          740 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.418, whole genome shotgun sequence.
ACCESSION   DADLSB010000411 DADLSB010000000
VERSION     DADLSB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.418"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(175..>740)
                     /locus_tag="JN331_004463"
     CDS             complement(175..>740)
                     /locus_tag="JN331_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAY7262351.1"
                     /translation="IEKAHHDVHELFFQVFDKGAMEDGEGVHIDFKNTTLLLTTNAGA
                     ELISQLCEDPTLMPDSAGLKEALMPELRKHFPAAFLGRVTVIPYLPLDETARGVIARL
                     HLGRLMDRMAEQHGVSLVYGDELITHIVSRCPMHETGARLLIGYIEQHILPQLSRYWL
                     QAMTEKTAITQIRIRVDDDEQVIFETD"
BASE COUNT          194 a          202 c          172 g          172 t
ORIGIN      
        1 gggagggcga tatgttacag agatactgac ccttgcacgc gcattaccaa gcgcgcgcaa
       61 aaaattatct actgatgcac gaaaagtccc ctgcaaatca ttagtggaag aacttccagg
      121 aaagcgactg acccacagtg cgccacttaa ttcttttgcc ataaggatgc tccattaatc
      181 cgtttcaaaa ataacctgtt catcgtcatc aacacgaatg cggatttgcg tgatggccgt
      241 tttctctgtc atcgcctgca accagtaacg ggacagttgt gggaggatat gttgttcgat
      301 atagccgatg agcaaacgag cgccggtttc gtgcatcggg cagcgcgaca caatatgcgt
      361 aatcaattca tcgccataca ccagtgaaac gccatgctgc tccgccatcc gatccatcaa
      421 tctgccaaga tgcaggcggg caataacacc acgcgcggtc tcatccagcg gtaaataagg
      481 aataaccgtc acacgcccca ggaaagccgc cgggaagtgt ttacgtaact ccggcattaa
      541 cgcctctttt agtcctgccg aatcaggcat cagcgtcgga tcctcacaga gctggctgat
      601 aagttccgct cccgcgttag tagtcaacaa cagcgtggta tttttgaaat cgatgtggac
      661 accttcgcca tcttccatcg cccccttatc aaacacctgg aagaacagct cgtggacgtc
      721 gtgatgggct ttttcaattt
//
LOCUS       DADLSB010000412          734 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.419, whole genome shotgun sequence.
ACCESSION   DADLSB010000412 DADLSB010000000
VERSION     DADLSB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.419"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            72..638
                     /locus_tag="JN331_004464"
     CDS             72..638
                     /locus_tag="JN331_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7262352.1"
                     /translation="MLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT          200 a          183 c          183 g          168 t
ORIGIN      
        1 tgcctgaact gcttgaacgt attggggcat atcatcaggg ccgtgaactg acccggcatg
       61 ccgttctgct gatgctgcat gtgttcattc gctccagtga actgcgtttc gcccgctggt
      121 cagagattga tttcacaaac cgagtctgga cgatacccgc gacgcgagaa cccattattg
      181 gcgtgcgtta ttccggccgc ggggcaaaaa tgcgaatgcc gcatatcgtc cccctctcag
      241 aacagtccat cgccattctg aaacagatta aggatatcac cggtaataat gaactgatct
      301 tccccggcga ccataacccg tataagccaa tgtgtgaaaa cacggtcaat aaggcactgc
      361 gggtgatggg ttacgacacg aaaaaggata tctgcggtca cggcttccgg gcaatggcat
      421 gcagtgcgct gatggaatcg ggtttatggg caaaggacgc agtagaacgc cagatgagtc
      481 atcaggagcg caataccgtg cgcatggctt atattcataa ggcagagcac ctggaagccc
      541 gcaaagcgat gatgcagtgg tggtcggatt atctggaagc atgccgagaa tcttatgcac
      601 cgccttatac aattggtaaa aataagttta tcccatagta tactaacgaa tcccacactc
      661 tggcaaatta ccaccacctc tacaggaggt ggttctatat agacttttat caacttcatg
      721 acgaaaaaca ccta
//
LOCUS       DADLSB010000413          724 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.420, whole genome shotgun sequence.
ACCESSION   DADLSB010000413 DADLSB010000000
VERSION     DADLSB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.420"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..517)
                     /locus_tag="JN331_004465"
     CDS             complement(<1..517)
                     /locus_tag="JN331_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY7262353.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSR"
BASE COUNT          202 a          169 c          176 g          177 t
ORIGIN      
        1 ccctcgagcc gctatgaacc agcagttgca gatgctgcgc atccagacct gccagtgcaa
       61 acagttcagc gttgatgatt tgatcaactt gttgcagttc agcgaaatga ttgccaccac
      121 cgatagagcc aagcgagctg cgccacggat gctgtgcaaa tgctgacggc aggttttcct
      181 ccagccagct ttcttcagca acgtcatcca aagctgataa tcgcttttca aacttatcgg
      241 cgttgtattt gcgagcgaga atatctgttt gccatagcgc catgccacag ccgatatcat
      301 tgccgaccag tgctgggtaa aaacggcctg cggagaagaa cgctgcgcca atcgggtagc
      361 cgcgtccggg gtgtaaatct ggcatcccca ctacgcgctg catgttgggt aaatttgccg
      421 tggtgtgtaa ttgttggatc gctaaactct cgatccacag gtcatcagat gcgatggtaa
      481 ataccgcatc ggataaggga cgaatataat tgcccatata ccattccgta aatagactcg
      541 tcatacttca agttgcatgt gctgcgactt gaattattga gagtaacaat taaaaaatca
      601 ggaaatggca gacgaatcca tggcaagaaa aaaagaatta atcctggaaa ggtctgcaaa
      661 gagtcattga taaaggattt gtcatgattt acctccttgc agtttgtgga ataagaaaac
      721 gcgc
//
LOCUS       DADLSB010000414          713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.421, whole genome shotgun sequence.
ACCESSION   DADLSB010000414 DADLSB010000000
VERSION     DADLSB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.421"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>713
                     /locus_tag="JN331_004466"
     CDS             <1..>713
                     /locus_tag="JN331_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7262354.1"
                     /translation="AGACRFVFNRALALQNENHEAGNKYIPYGKMASWLVEWKNATET
                     QWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPRFKKRGQNAAFRYPQGVKLDQENSR
                     IFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPVHPSASMIGLDA
                     GVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCI
                     ANIRRDYLHKVTTTVSKNH"
BASE COUNT          204 a          181 c          176 g          152 t
ORIGIN      
        1 cgcaggcgca tgtcgtttcg ttttcaatcg tgctctggcg cttcagaatg agaatcatga
       61 ggccggtaat aaatacatcc cttacgggaa aatggcttct tggctggttg agtggaaaaa
      121 cgccactgaa acgcaatggc ttaaagattc gccctcacag ccattgcaac agtcactgaa
      181 agaccttgag cgggcttaca aaaacttctt ccagaagcgg gctgcttttc cccgattcaa
      241 aaagcgggga cagaatgctg cattccgcta cccgcagggc gttaagctcg atcaggaaaa
      301 cagccgtatt tttctgccga aacttggctg gatgcgctac cggaatagcc gtcaggtcac
      361 gggagttgtg aaaaatgtca ctgtcagcca gtcctgcggt acgtggtaca tcagtattca
      421 gacagaaagt gaagtatcaa ctccggttca cccttcagca tcaatgatcg ggctggatgc
      481 tggcgtggct aaactcgcca cgctgtcaga tggcacagtc tttgagcctg taaacagttt
      541 tcagaaaaac cagaagacgc tggcgagact tcagcgacag ttaagccgga aggtcaaatt
      601 cagcaacaac tggcagaagc agaaacgcaa aatacagcga ctgcattcct gtatcgcaaa
      661 tatccgcagg gactaccttc acaaagtcac aacgaccgtc agcaaaaacc acg
//
LOCUS       DADLSB010000415          680 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.422, whole genome shotgun sequence.
ACCESSION   DADLSB010000415 DADLSB010000000
VERSION     DADLSB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.422"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            135..564
                     /locus_tag="JN331_004467"
                     /pseudo
     CDS             135..564
                     /locus_tag="JN331_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          172 a          137 c          222 g          149 t
ORIGIN      
        1 attcctgccg gtatcagcgc cgacggcatg tattgccctg aatgcggtac ggttcactgg
       61 ccggatggcg ttattccgcc tttctgatgc ttccccgttt tgccgatatt tttcagcagg
      121 gaaaccgatg gcttaactgg ctggagaagc agccggtgca gatgagccgc ctggaacatt
      181 atgccggtca ggatgaaatt ggcctgcgtt acaacagcca ccggacaaaa cgagaagaaa
      241 accttgtgat gagtggcgac gagtttatgg aaaggttctc ctggcatgtg gcggataagg
      301 ggttccggat ggtgatacgg ggtcctgagt ccggtgaagc ggcgattact ggaagatgtg
      361 gtgtacgcca taacggagac agtgagaaaa acggcgaggc aaatcacaag gagagggatg
      421 tatcagcagt gactgaaggt tgacccgctg aagtgcgagc tgtgcggagg tcagatgcgg
      481 tttacggggc tgaagagggg ttaccgtctg gcagagcagg ttatgatgca tgagccgctg
      541 gctcgaatac ggtggtgcgg ctgagagtcg cagaggggaa gttgcgtcca tgttgagggg
      601 aacggagcaa aaaaccatca gagatacatt gaatcaatca atgtaagcaa tattaagtgt
      661 ctataccgat tacttgatga
//
LOCUS       DADLSB010000416          658 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.423, whole genome shotgun sequence.
ACCESSION   DADLSB010000416 DADLSB010000000
VERSION     DADLSB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.423"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..566
                     /locus_tag="JN331_004468"
                     /pseudo
     CDS             1..566
                     /locus_tag="JN331_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          169 a          136 c          161 g          192 t
ORIGIN      
        1 gctggtttga gttacaaaga ttttggttca ttcgactaca gtcgtaatgt cggtgtcgcg
       61 tatgatgcag aagcatttac tgatatgttt gttgagtggg gcggtgactc atgggctggt
      121 actgacctgt tcatgaccaa ccgtaccaac ggtgttgcaa cctatcgtaa cacagatttc
      181 tttggcatgg ttgaaggtct aaattttgct ctgcaatatc agggtaaaaa tgaaggtaca
      241 ggcaactaca aagcaaatgg cgatggccat ggtttatctg caacggtttc tcatttgctg
      301 gtgcttatgc aaactctgac cgtactgatt ggcagtctgg tgatggtaaa ggcgaaagag
      361 ccgaagtttg ggcgttgtca acaaaatatg acgctaacaa tgtttatgct gctgtgatgt
      421 acggtgaatc tcacaacatg aattccgacg acggtgatgt tgtgaacaaa acacaaaact
      481 tcgaagctgt tctgcaatat cagttcgact ttggtctgcg tccatccatc ggttactcct
      541 attctaaagc actggacgta gcaggttgga tttgccccta tatttccaga cacctgttat
      601 aacttaaccc attactggct tgctgccgta gatattcccg tggcgagcga taacccag
//
LOCUS       DADLSB010000417          654 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.424, whole genome shotgun sequence.
ACCESSION   DADLSB010000417 DADLSB010000000
VERSION     DADLSB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.424"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>654
                     /locus_tag="JN331_004469"
     rRNA            <1..>654
                     /locus_tag="JN331_004469"
                     /product="23S ribosomal RNA"
BASE COUNT          183 a          144 c          200 g          127 t
ORIGIN      
        1 cgggacacgt ggtatcctgt ctgaatatgg ggggaccatc ctccaaggct aaatactcct
       61 gactgaccga tagtgaacca gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga
      121 gtgaaaaaga acctgaaacc gtgtacgtac aagcagtggg agcacgctta ggcgtgtgac
      181 tgcgtacctt ttgtataatg ggtcagcgac ttatattctg tagcaaggtt aaccgaatag
      241 gggagccgaa gggaaaccga gtcttaactg ggcgttaagt tgcagggtat agacccgaaa
      301 cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga ggaccgaacc
      361 gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc aatcaaaccg
      421 ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt catctccggg
      481 ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg caaactgcga
      541 ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt cgtgaagagg
      601 gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga aacg
//
LOCUS       DADLSB010000418          652 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.425, whole genome shotgun sequence.
ACCESSION   DADLSB010000418 DADLSB010000000
VERSION     DADLSB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.425"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            219..>652
                     /locus_tag="JN331_004470"
     CDS             219..>652
                     /locus_tag="JN331_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262355.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGAL"
BASE COUNT          150 a          144 c          158 g          200 t
ORIGIN      
        1 cctaaaacaa caatatggac atcttaacga tactctgcgc ctgcgtgtgc ggaacagaaa
       61 cccttacccg atatacttgt tatgccagag gtagcaattg cgttagcggc gtactgttgc
      121 tgtaaagtat aacgcctata ttttacagtt cagtcctctt ttgcttatta caaccttaag
      181 tatatcaagc atataaagat aataagagac tgaacaatat ggttacctgg acccaaatgt
      241 atatgccgat gggaggactg gggctatccg ctctggtcgc cctgatcccg ataatattct
      301 tcttcgttgc actcgcggta ttacgtctga aaggacatgt cgctggagca ataaccctta
      361 tattatctat cctgattgcg atattcgcct ttaaaatgcc gattgatatg gcatttgctg
      421 ctgcgggcta tggctttatt tatggattat ggccaattgc gtggattatt gtcgcggcgg
      481 tgttcctgta taaattaacc gttgccagcg ggcagttcga tattatccgc agctcggtta
      541 tctccatcac cgacgatcag cgtttgcagg tgttactgat tggtttctcc tttggtgcgt
      601 tgctggaagg agcggctggc tttggtgcgc cggtggcgat taccggtgcg ct
//
LOCUS       DADLSB010000419          650 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.426, whole genome shotgun sequence.
ACCESSION   DADLSB010000419 DADLSB010000000
VERSION     DADLSB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.426"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..578)
                     /locus_tag="JN331_004471"
     CDS             complement(<1..578)
                     /locus_tag="JN331_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7262356.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAA"
BASE COUNT          165 a          164 c          155 g          166 t
ORIGIN      
        1 gcggccacat tttcgacttc ctccggagag gaaaatttac ggaacaggat ccagttccac
       61 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat gttcgcaaat
      121 gcgatagatt ttgccctgcg cccacgacta ccatcaggat aaatatgctc ggtgtaaaat
      181 ccggcctgaa tggttaccca ctcgcggaaa gcgtcaaacg actttagcaa tgccgtatcc
      241 cgggttctgc atgtcgcaac tgtattaagg tattgctctg cggcatcact cagggctgga
      301 gtgtgttccc gacctactga ttcgcacagg taatcaacga agcctgatac cagttttcgt
      361 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat caaatcccag ttgcaggagt
      421 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      481 tcacctattt tgatttgatg caaaaaatcg caactctccg gcgtcgccgg gagaagtaat
      541 ccggaagagg tttgtttgac cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      601 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac
//
LOCUS       DADLSB010000420          620 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.427, whole genome shotgun sequence.
ACCESSION   DADLSB010000420 DADLSB010000000
VERSION     DADLSB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.427"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..213
                     /locus_tag="JN331_004472"
     CDS             <1..213
                     /locus_tag="JN331_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262357.1"
                     /translation="PQDVQHFLSNAEMCQHLAGEWDSSLPEKDKRDIEKGVDTWCPPA
                     KKSLPVLREKYKENKEIIKILSAYDF"
     gene            275..>620
                     /gene="tssB"
                     /locus_tag="JN331_004473"
     CDS             275..>620
                     /gene="tssB"
                     /locus_tag="JN331_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HAY7262358.1"
                     /translation="MAINNSAQKFIARNRAPRVQIEYDVEIYGSEKKIELPFVMAVLA
                     DLAGKPREELPPVTDRKFLDIDIDNFNERMKAIAPRVAFAVPNTLTGEGQLMVDITLE
                     NMDDFSPAQIARK"
BASE COUNT          168 a          142 c          170 g          140 t
ORIGIN      
        1 ccacaggatg tgcagcattt tctgagtaat gcggaaatgt gccagcatct tgctggtgaa
       61 tgggacagtt ctttaccaga gaaggataaa agagacatcg aaaagggggt tgatacctgg
      121 tgtcctccgg cgaagaaatc actccctgtg ctgcgggaaa agtacaaaga aaacaaagag
      181 attataaaaa tactctctgc gtatgatttt taaactgtac cgtcggacca ctccccaggt
      241 ggtcgtcggc tgactgataa ggaaatgaag gattatggct atcaacaaca gcgcgcagaa
      301 gtttattgcc cgcaaccgcg cgccccgcgt gcagattgag tatgacgttg agatttacgg
      361 ctccgagaaa aaaatcgaac tgccgtttgt catggcggtg ctggccgatc tggccggtaa
      421 accacgcgaa gagctgccac cggtcaccga tcgtaaattc cttgatattg atatcgataa
      481 cttcaacgag cggatgaaag ccattgcgcc gcgcgtggcg tttgctgtcc ccaatacgct
      541 gacgggcgaa gggcagttaa tggtcgatat cacgctggag aatatggacg atttttcgcc
      601 tgcgcagatt gcccgtaagg
//
LOCUS       DADLSB010000421          616 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.428, whole genome shotgun sequence.
ACCESSION   DADLSB010000421 DADLSB010000000
VERSION     DADLSB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.428"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..489
                     /locus_tag="JN331_004474"
     CDS             <1..489
                     /locus_tag="JN331_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7262359.1"
                     /translation="EKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSV
                     RQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKH
                     FLTIEVRPPMDACIASADNKMIARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADP
                     YP"
BASE COUNT          181 a          124 c          138 g          173 t
ORIGIN      
        1 gaaaaaatgg ccgccatgtt tcatcagcaa cgaaatccag tgattgatta tgtctggaat
       61 tctgtacggc gtaaattcgg ggggcgctta cattcccggg aggatgggat aaaaccattt
      121 attcagtcag tacgccaggg atactggggg tattaccttc cagatcagga tcatggtcct
      181 gaatacagtg aatttgctga tttttttgcg acctataaag cgacattacc aattattgga
      241 cgtctgatga acatcagtca ggctatgatt ataccgcttt tcccggttta tgatgaaaaa
      301 aaacatttcc tgactattga agttcggcca ccaatggatg catgcattgc cagcgcggac
      361 aataaaatga ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg gtcacatccg
      421 gaacagtata tctgggtttt aaaattgtta aaaacgcgca aatcaaacga agcggacccg
      481 tacccttgaa attataaagt acaggtattt ccactagttg tttcttacag gttaccaatc
      541 gaaacacatc ccccttccgt gtgttgcccg tttggcacgg aggactgtgt agatgcagtt
      601 cgttaaattt aattta
//
LOCUS       DADLSB010000422          611 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.429, whole genome shotgun sequence.
ACCESSION   DADLSB010000422 DADLSB010000000
VERSION     DADLSB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.429"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            31..393
                     /locus_tag="JN331_004475"
     CDS             31..393
                     /locus_tag="JN331_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY7262360.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LRREMGLEEMTGFSKTTF"
BASE COUNT          192 a          141 c          138 g          140 t
ORIGIN      
        1 tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa
       61 cgtctcagcg atcgcctgga cgccatccgc caccagcagg ccgatctgtc gctggttgaa
      121 gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct
      181 cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg
      241 ccgttccagc gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt
      301 gttcgcggac tggtggttgt gcacccaatg accgcgctga ggcgcgaaat gggcctggaa
      361 gagatgactg gattttctaa gactacgttt taagcccctc agacgactgg ttcgatcgta
      421 tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt ctactcgcct
      481 aattaccaaa caattcactc cctttacttc gatttaccgc tggttgatat tctgaaaatg
      541 catttttacc gaaatagcca tctataaaca ataaaaatat atagaagata tcatttgtta
      601 attaacaagc a
//
LOCUS       DADLSB010000423          581 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.430, whole genome shotgun sequence.
ACCESSION   DADLSB010000423 DADLSB010000000
VERSION     DADLSB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.430"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..120
                     /locus_tag="JN331_004476"
     CDS             <1..120
                     /locus_tag="JN331_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262361.1"
                     /translation="DSSLYAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(132..341)
                     /locus_tag="JN331_004477"
     CDS             complement(132..341)
                     /locus_tag="JN331_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262362.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT          158 a          134 c          121 g          168 t
ORIGIN      
        1 gactccagtt tgtatgcaat gcaaattcag cgctatcaac aggctgtgcg atcaacaatg
       61 gtcctttcac tgactgagct gatttccaaa atcagcttaa aaaaagcctt tcaaaagtaa
      121 gcaacgtctg cttactgccc ctctacctgc ttcggccgat aaagccgacg ataatactcc
      181 agacgttgta gatataacgg cacgctttca tcgggtattc cggacggaat cgcgttacgg
      241 ggaggaagtt ttttcagata ctcccggaac gcctggcttg atgccatgaa atctacggct
      301 ttatcgataa gaagcggaac gttttcacct aatttgccca tgatgatatc tccgtattac
      361 cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt tcccggtaaa gatgacatta
      421 aggaaaacgt gctgaatcct caataatcat ccgctgctgt ttattatctt tttctataga
      481 tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg ttaatgatta cgttgcagaa
      541 ctattacaat gtcatcagtt gtgaacaaag cacctggtcg c
//
LOCUS       DADLSB010000424          578 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.431, whole genome shotgun sequence.
ACCESSION   DADLSB010000424 DADLSB010000000
VERSION     DADLSB010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.431"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..>578)
                     /gene="virK"
                     /locus_tag="JN331_004478"
     CDS             complement(<1..>578)
                     /gene="virK"
                     /locus_tag="JN331_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7262363.1"
                     /translation="WSRGQYILEHYRFVQNLPEKYSEFLFPQKSVSLVQFIGKDGEDF
                     DIQCSPSGFDREGELMLSLFFNKIVIARLTFSVILTQNGHTAFIGGLQGAPKNTGPDV
                     IRCATRACYGIFPKRIIFEAFCALMKACNISECLAVSEHSHVFRQLRYWYQKRKTFVA
                     VYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIA"
BASE COUNT          177 a          139 c          108 g          154 t
ORIGIN      
        1 gctatatcgc ttagcggctt tcgaatcacc tgggttggta atctgtacca gtcaccacag
       61 gtttttcctg ctacagactc ccagaaatca ctatagaccg caacaaaggt tttgcgtttc
      121 tgataccagt atctcaattg ccggaaaaca tgactgtgct cactgactgc cagacactca
      181 gagatattgc aagctttcat caacgcacaa aatgcttcaa aaataatccg ttttgggaaa
      241 atcccataac aggcccgggt tgcgcaccga ataacatcag gtccggtatt tttaggggct
      301 ccctgtaatc cgccaataaa agcggtgtgt ccattttgag tcagaatgac tgaaaatgtt
      361 aaccgggcaa tcactatttt attgaaaaac aatgacaaca tcaactctcc ttcccggtca
      421 aaaccactgg gagaacactg gatatcaaaa tcctctccat ctttcccgat gaattggact
      481 aatgataccg atttttgtgg aaacaaaaat tcagaatatt tttctggcaa attttgcaca
      541 aagcgataat gctcaagaat atattgcccc cgggacca
//
LOCUS       DADLSB010000425          573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.432, whole genome shotgun sequence.
ACCESSION   DADLSB010000425 DADLSB010000000
VERSION     DADLSB010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.432"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(1..365)
                     /locus_tag="JN331_004479"
                     /pseudo
     CDS             complement(1..365)
                     /locus_tag="JN331_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            288..539
                     /locus_tag="JN331_004480"
     CDS             288..539
                     /locus_tag="JN331_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262364.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
BASE COUNT          156 a          131 c          142 g          144 t
ORIGIN      
        1 gccagtgttg ttgcgccttg ctgccaacga agcgccactg ctcatcaagt tcacagataa
       61 gggcaacatc agcgtgagcg actggcgcag atgttatctg ccggggcgag agctttttaa
      121 tgtccgtatg acggtattga tgccgatttt cagtgttctg gcggtataac agccccctgc
      181 gccgttatgt gccatttcaa cgatctgctc cttaacgccg agtttctggc ttcataagag
      241 taagtgagct ggaaaacacg tttacatgag cgacaacgga aacgctcatg ccgggaacaa
      301 ctacggccat gtcgataaac ttcatcggaa ttacagcgcg gacaatgaac agtaacggta
      361 gtcatggaaa acctcaaata ccagtagatt accagaaact tctatcgatt gaagacatta
      421 ccttttgccg caataggtgg ggtaacattg gtgaaaacgc attacgccgg gtggcagttg
      481 gaaaaaaatt atcttttttc ggctctgatc gtggtggaga aaatgcggca ataatctaaa
      541 tagctgcgct taataccgct acacttttgc cag
//
LOCUS       DADLSB010000426          573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.433, whole genome shotgun sequence.
ACCESSION   DADLSB010000426 DADLSB010000000
VERSION     DADLSB010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.433"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..200
                     /locus_tag="JN331_004481"
     CDS             <1..200
                     /locus_tag="JN331_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY7262365.1"
                     /translation="EYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLSA
                     RFNSAMDIPAQGLCRIPEKRS"
     gene            210..536
                     /locus_tag="JN331_004482"
                     /pseudo
     CDS             210..536
                     /locus_tag="JN331_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT          132 a          147 c          164 g          130 t
ORIGIN      
        1 tggagtacgc cgaagccgca agcgtcacac cgccaacagt cgatgatgcc ctgcgtaccc
       61 gactggctgc gcattttgac gctcaggcgc tcaccgaact gacggcattg atcggcctgc
      121 aaaacctgtc ggctcgtttt aattctgcca tggacattcc cgctcagggg ctgtgccgta
      181 ttccggaaaa acgttcttaa ggggggaaga tgcgccattg tgggtggttg ctgggattgt
      241 tatcgctgtt ttctttggca acacatgcca gtgactggca ggaaattaaa aatgaggcca
      301 aagggcaaac cgtctggttt aacgcctggg gcggcgatac cgcaattaac cgctatctcg
      361 actgggttag cggcgagatg aaaacccatt acgctataaa cctgaagatt gttcgtctcg
      421 cggatgccgc agacgcggtg aagcgcattc agaccgaagc tgctgccggt cgtaaaacgg
      481 gcggctcggt ggatctgctc tgggtgaacg gcgaaaactt ccgcacctta aaagagtaat
      541 gccagtcagt taagcaactg actggctctt ttt
//
LOCUS       DADLSB010000427          554 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.434, whole genome shotgun sequence.
ACCESSION   DADLSB010000427 DADLSB010000000
VERSION     DADLSB010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.434"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>554
                     /locus_tag="JN331_004483"
     CDS             <1..>554
                     /locus_tag="JN331_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7262366.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEM"
BASE COUNT          125 a          134 c          162 g          133 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcttggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgtt
//
LOCUS       DADLSB010000428          549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.435, whole genome shotgun sequence.
ACCESSION   DADLSB010000428 DADLSB010000000
VERSION     DADLSB010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.435"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            63..>549
                     /locus_tag="JN331_004484"
     CDS             63..>549
                     /locus_tag="JN331_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7262367.1"
                     /translation="MLYAARKRGLEIGIFCTIHTLRLHFEEHLPLVVAGRRLGVPKST
                     VCSMFVRFRKAGLSWPLPAGMSERELDARLYGSASTVPVVLTESTVMPEVPGVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LL"
BASE COUNT          120 a          159 c          146 g          124 t
ORIGIN      
        1 acggcacgga accacggcag cttgacggct gcaatgacgt gtgtcgtctg gcggtggaga
       61 atctgctgta tgcagcccga aaacgggggc tggaaatcgg catcttctgc accatccaca
      121 ctttacgcct tcactttgaa gaacatttac ccctcgttgt ggccggacgt cgcctgggcg
      181 taccaaaatc aacagtttgt agtatgtttg tgcgcttccg caaagctggc ctttcatggc
      241 ctctgcccgc aggtatgtcg gagcgggaac ttgacgcccg tctttacggg agtgcctcca
      301 cagtacctgt cgtacttact gaaagtaccg ttatgcccga agtccccggg gtaaaaaagc
      361 gtccccggcg ccccaacttc ccttatgagt tcaaaatcgc cttagtggag cagtcactgc
      421 agcccggagc ctgtgtggcg cagattgccc gtgaaaatgg aatcaacgat aacctgctct
      481 tcaactggcg ccatcaatac cggaaaggtg gcctgctgcc ttccggaaaa aatatgccgg
      541 cactgcttc
//
LOCUS       DADLSB010000429          549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.436, whole genome shotgun sequence.
ACCESSION   DADLSB010000429 DADLSB010000000
VERSION     DADLSB010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.436"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..207
                     /locus_tag="JN331_004485"
     CDS             <1..207
                     /locus_tag="JN331_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7262368.1"
                     /translation="VYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHM
                     DLYDGKAYVAEAISVLAPFFEETL"
     gene            207..>549
                     /locus_tag="JN331_004486"
     CDS             207..>549
                     /locus_tag="JN331_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7262369.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          144 a          113 c          148 g          144 t
ORIGIN      
        1 gtgtacctga ctcagccgat gcagattgtg gcgggcagcc aggcagggag caaatggatg
       61 agtgacgacc tgtacgatcg ggcatcaagc caggataaac gctatcacat tgttgaaggc
      121 gcaaaccata tggatcttta tgacggcaaa gcctatgtgg ctgaagcaat ttcagtatta
      181 gctccgttct ttgaggaaac tctgtaatga aacatattca aatccgcaat agtgatatgg
      241 cctggcatat tgctgccaat attcagtttc caccaaattt tgatgagtca aaacagtatc
      301 cggcgattat cagcgtccat ccttttggca gttgtaagga gcagacgtct ggcaatatct
      361 atggcaaggc attggctgaa aaaggctatg tcgttctcgc ttatgacgcc agtttccagg
      421 gagaatctgg tggggagcct cgttggatag aagatccgac acagcgcgta gaggatatta
      481 gccgtgttat cgattatgca gtaactctgc catatgttga tgctgaacgc attggcgtgc
      541 tgggtgtgt
//
LOCUS       DADLSB010000430          549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.437, whole genome shotgun sequence.
ACCESSION   DADLSB010000430 DADLSB010000000
VERSION     DADLSB010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.437"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..463
                     /locus_tag="JN331_004487"
     CDS             <1..463
                     /locus_tag="JN331_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7262370.1"
                     /translation="LGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLIDH
                     QAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYDQ
                     VERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          142 a          106 c          120 g          181 t
ORIGIN      
        1 tctgggtggg gagatggata aagatatgat cgccattcga atcgggccag atataccaat
       61 ggctattgtt ggctcaccgg attatttttc tcgtcgaagt gctccaacgt cagtgtcaca
      121 attaatagat catcaggcaa ttaatttgta tcttcccaca tcgggtacag caaatcgctg
      181 gagattaata cgcggtggac gtgaagttcg tgttcgcatg gaaggtcagc ttttactgaa
      241 tacgatagac ctgatcattg atgctgcaat tgatgggcat ggattggcgt atctacctta
      301 tgatcaggtt gagcgggcta ttaaagaaaa aaaactgata cgtgttttgg ataaattcac
      361 tccagattta cccggttatc acctgtacta tccacaccgt cgacatgctg gctcggcatt
      421 ctcattattt atagataggc tgaagtataa aggtgctgtt tagcactact tgctgataca
      481 ttaatttaat tcttctctta acgtattctc agttcctttc aacgttttgg ttgtttttta
      541 ttcttcgta
//
LOCUS       DADLSB010000431          548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.438, whole genome shotgun sequence.
ACCESSION   DADLSB010000431 DADLSB010000000
VERSION     DADLSB010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.438"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>548
                     /locus_tag="JN331_004488"
     CDS             <1..>548
                     /locus_tag="JN331_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7262371.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLR"
BASE COUNT          178 a          117 c          120 g          133 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagac
//
LOCUS       DADLSB010000432          546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.439, whole genome shotgun sequence.
ACCESSION   DADLSB010000432 DADLSB010000000
VERSION     DADLSB010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.439"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(54..242)
                     /locus_tag="JN331_004489"
     CDS             complement(54..242)
                     /locus_tag="JN331_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262372.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            358..546
                     /locus_tag="JN331_004490"
                     /pseudo
     CDS             358..546
                     /locus_tag="JN331_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          138 a          131 c          162 g          115 t
ORIGIN      
        1 gtgaacagac gtttcagcgg gcggggagta aaagcggaca acatgaggac ggattaaccc
       61 ccggtatcct gtagccgggt aagggctgct gtgtcgccac ggcgctccag gatggaggcc
      121 gcgaccatcg caggtggtgg gtccatgggt ttgagcatct tcatccagtc ccattcacgt
      181 tgtgagagct tctcagcacc aacagagagc agaacggtat ctatactttt gcgggtcaac
      241 ataagaggat gagtccttaa taaaacaggt cctcagagca tacatattct gcggagcgtg
      301 cggcaacaac aggacgaaca ggggcggttt tcaatttgca gccgtcaggc tgccgtggtt
      361 cagcgcctga tgggaatcct ctcacttaag gcagcgatta aggtcaagcg ataccgctct
      421 tacagaggag aggtagggca aaccgccccc aatgttctcc aaagagattt caaggctacg
      481 cggccaaacg agaagtgggt taccgatgtt actgaatttg cagtcaatgg gcgcaagctg
      541 tatttg
//
LOCUS       DADLSB010000433          542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.440, whole genome shotgun sequence.
ACCESSION   DADLSB010000433 DADLSB010000000
VERSION     DADLSB010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.440"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..91
                     /gene="yjdI"
                     /locus_tag="JN331_004491"
     CDS             <1..91
                     /gene="yjdI"
                     /locus_tag="JN331_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY7262373.1"
                     /translation="WVMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            103..375
                     /locus_tag="JN331_004492"
     CDS             103..375
                     /locus_tag="JN331_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7262374.1"
                     /translation="MEICEGHNNFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT          173 a           95 c          146 g          128 t
ORIGIN      
        1 gtgggtcatg ccggatgaag tcgacgtcgc cactgtggtt aaagtgattg atacgtgccc
       61 gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat atgcgaaggg
      121 cacaataatt tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
      181 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
      241 caagggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
      301 aaaattatcc cattatgtcc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
      361 gatattcgca gttgatggga gagtacagag tcacgatatt tttcttgctc tccgcggtgt
      421 gatgcaggag agcatctgaa aggtaggggg atgcacaaag aatgggcaga gagcgcgttt
      481 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
      541 at
//
LOCUS       DADLSB010000434          540 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.441, whole genome shotgun sequence.
ACCESSION   DADLSB010000434 DADLSB010000000
VERSION     DADLSB010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.441"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(252..>540)
                     /locus_tag="JN331_004493"
     CDS             complement(252..>540)
                     /locus_tag="JN331_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7262375.1"
                     /translation="IGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQ
                     GFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSRES"
BASE COUNT          169 a           94 c          100 g          177 t
ORIGIN      
        1 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
       61 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
      121 gtttttaatt atgtttttgt aataagtttg aagacgcaaa aaaataatat aataacaaaa
      181 atcctccagc cttgtaggcc agaggataaa atacttaatt gaggaaaaca tcagggaaaa
      241 gtatttctga cttaactttc ccggctggag agtgaatcaa tgtaatcagc gtaccattgc
      301 atcatttcac ggcggccatc gagatacagg gcatgattat aggtacccct gatggaattt
      361 ttatccacat gagctaactg catttcaatc caggcggaat taaagccttg ttcatgcaga
      421 attgtactca tggtgtgtct gaaaccgtgc cctgtgagtc ttcccttata tccaagtaat
      481 tcaattactt ggttaatgct ttccttacta attggtttgc ggggatcatt tctaccgata
//
LOCUS       DADLSB010000435          522 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.442, whole genome shotgun sequence.
ACCESSION   DADLSB010000435 DADLSB010000000
VERSION     DADLSB010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.442"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            161..>522
                     /locus_tag="JN331_004494"
     CDS             161..>522
                     /locus_tag="JN331_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7262376.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEIL"
BASE COUNT          160 a           95 c          112 g          155 t
ORIGIN      
        1 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
       61 tatcctgtac actcagaaca tcaggattta aattaaggtt tgatgtattc tctgaataaa
      121 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
      181 gtttattcct gaatttaaga aaaattacct ttcccctgtt tactggtcta catggttcct
      241 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      301 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
      361 tttatcgctt tgtttcccgg aaaagagtga tacagaacgg gaaataattg tcgacaaaat
      421 gtttgccaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
      481 aaagttccag aaacgagtgt tctggaaagg cctcgaaatt ct
//
LOCUS       DADLSB010000436          520 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.443, whole genome shotgun sequence.
ACCESSION   DADLSB010000436 DADLSB010000000
VERSION     DADLSB010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.443"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..444
                     /locus_tag="JN331_004495"
     CDS             <1..444
                     /locus_tag="JN331_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7262377.1"
                     /translation="VGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKT
                     TLQRGVMAPRLLIIDEIGYLPFSQKEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTF
                     AGDAALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          148 a          126 c          130 g          116 t
ORIGIN      
        1 gtggggaaaa cccatctggc aatagcgatg ggctatgaag cagtccgtgc aggtatcaaa
       61 gttcgcttca caacagcagc agatctgtta cttcagttat ctacggcaca acgtcagggc
      121 cgttataaaa cgacgcttca gcgtggagta atggcccccc gcctgctcat cattgatgaa
      181 ataggctatc tgccgttcag tcagaaagaa gcaaaactgt tcttccaggt catcgctaaa
      241 cgttacgaaa agagcgcaat gatcctgaca tccaatctac cgttcgggca gtgggatcaa
      301 acgttcgccg gtgatgcagc actgacctca gcgatgctgg gccgtatctt acaccactca
      361 catgtcgttc aaatcaaagg agaaagctat cgactcagac agaaacgaaa ggccggggtt
      421 atagcagaag ctaatcctga gtaaaacggt ggatcaatat tgggccgttg gtggagatat
      481 aagtggatca cttttcatcc gtcgttgaca caacactggc
//
LOCUS       DADLSB010000437          512 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.444, whole genome shotgun sequence.
ACCESSION   DADLSB010000437 DADLSB010000000
VERSION     DADLSB010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.444"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..453
                     /locus_tag="JN331_004496"
     CDS             <1..453
                     /locus_tag="JN331_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7262378.1"
                     /translation="IQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAELGDDSGWH
                     FFSQRNTDNSITFAVNGQVIPLNYGNFDARYKHRTEGVQDVRYGYEMYYSPGSNTVSW
                     RFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
BASE COUNT          149 a          110 c          128 g          125 t
ORIGIN      
        1 attcaggccg gaactgccag aaaattcacg ttatccagcg ccaacaactc cacgaaaaat
       61 gcagcgtttt atttgtgggg taatccatcc aggcctgttg ttgcagagct tggtgatgat
      121 tcaggctggc attttttcag ccagaggaat acagataaca gcatcacgtt cgccgtaaac
      181 ggacaggtaa ttccgttaaa ttacggaaac ttcgatgccc gctataaaca tcgaactgag
      241 ggggtacagg atgtgcggta tggttatgaa atgtattaca gccccggcag taacaccgtt
      301 tcgtggagat tttgcgcacc ttcgggacac gggctgtcag ggatggcgat atcggatacc
      361 ggccgtaact cagcggataa cgttgacggt gtgtattacc gaccactgca aaaactgatt
      421 aatggcacct ggtataacgt agcgagtatt taacaatgtt gcatttaaaa aatattactg
      481 cgggtaatcc gaaaacagca gaacaatatc ag
//
LOCUS       DADLSB010000438          511 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.445, whole genome shotgun sequence.
ACCESSION   DADLSB010000438 DADLSB010000000
VERSION     DADLSB010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.445"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(38..175)
                     /locus_tag="JN331_004497"
                     /pseudo
     CDS             complement(38..175)
                     /locus_tag="JN331_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            236..429
                     /locus_tag="JN331_004498"
                     /pseudo
     CDS             236..429
                     /locus_tag="JN331_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          138 a          130 c          111 g          132 t
ORIGIN      
        1 tgtttccgta aagtccctgc aatttctgaa ataatcaacg tacagccagg gcttcgttgt
       61 taatcgaacc ataataacga acttcgccaa gccgttcagg tgcagcaata gcaactgcca
      121 acgtttcttt atgtacatcc agcctgatgt aagctgcaat attttccgaa tgcataatga
      181 cctcctctgg aatatctcac caatgctata gtgagtatat aaaggtcata ccatctacat
      241 gttcttcagc aacgatcacg gtggagagcg tggtgcgtta ctgtacggtc tgatcggcac
      301 ctgccgtttg aacggtatcg atccggaagc gtatctgcgc catatcctga gtgcactgcc
      361 tgccggatat ggccttccaa ccgtgttgac gaactgctgc catggaacgt agttctcacc
      421 aataaataag cgtcaatact gtgctccgtt aacgcttacc actggcctat acggagcgac
      481 taaaagaagc aaaactgctg gcatcaacag g
//
LOCUS       DADLSB010000439          510 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.446, whole genome shotgun sequence.
ACCESSION   DADLSB010000439 DADLSB010000000
VERSION     DADLSB010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.446"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..489
                     /locus_tag="JN331_004499"
     CDS             <1..489
                     /locus_tag="JN331_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7262379.1"
                     /translation="KKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSV
                     RQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKH
                     LLTIEIRPPMDACIASADNKMIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADP
                     YP"
BASE COUNT          162 a          104 c          112 g          132 t
ORIGIN      
        1 aaaaaaatgg ccgccatgtt tcatcagcaa cgaaatccag tgattgatta tgtctggaat
       61 tcagtacggc gtaaattcgg ggggcgctta catgcccggg aggatgggat aaaaccattt
      121 attcagtcag tacgccaggg atactgggga tattacctcc cagatcagga tcatggtcct
      181 gaatacagtg aatttgctga tttttttgcg acctacaaag cgacattacc aatcattgga
      241 cgtctgatga acatcagtca ggctatgatt ataccacttt tcccggttta tgatgaaaaa
      301 aaacatctcc tgactattga aattcggcca ccaatggatg cttgcattgc cagcgcggac
      361 aataaaatga ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg gccacatccg
      421 gaacagtatg tctgggtttt aaaattgtta aaaacacgca aatcaaacga agcggacccg
      481 tacccttgaa attataaagt acaagtattt
//
LOCUS       DADLSB010000440          491 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.447, whole genome shotgun sequence.
ACCESSION   DADLSB010000440 DADLSB010000000
VERSION     DADLSB010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.447"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(77..>491)
                     /locus_tag="JN331_004500"
     CDS             complement(77..>491)
                     /locus_tag="JN331_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7262380.1"
                     /translation="FDCVARPWLTEAGSRSARGIWEIEFNHKLLRYIYGLTNQFTTYS
                     LRDCGSLRNPRTIRLYESLAQFKSSGLWVTTHAWLNDRFLLPESQQKNLAELKRSFLD
                     PALKQINEKTPLLAKYSIDDSGKFLFSIIDKQNPV"
BASE COUNT          136 a          100 c          120 g          135 t
ORIGIN      
        1 ccggtgctgt gtggaaattc gcccatcttc atgacaaaaa tttgtcaaat gacaggctca
       61 tgtgtgctga tttatgtcag acgggatttt gcttatcaat tattgagaac agaaattttc
      121 ctgaatcatc aatactatac ttagcaagta aaggtgtttt ctcatttatc tgcttgagtg
      181 caggatcaag gaaagatcgt ttcaactctg ccaagttctt ctgttgggat tccggcaaaa
      241 ggaaacggtc atttaaccaa gcatgagtag taacccataa gcctgaagat ttgaattgag
      301 caagactttc ataaaggcgg atcgtccgtg gatttcgaag actgccacaa tcgcggagcg
      361 agtaggtggt gaactggttc gtcaggccgt aaatgtaccg caggagttta tggttaaatt
      421 cgatttccca gataccacga gctgatcggc tgccagcctc tgttagccag gggcgcgcga
      481 cgcagtcaaa a
//
LOCUS       DADLSB010000441          469 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.448, whole genome shotgun sequence.
ACCESSION   DADLSB010000441 DADLSB010000000
VERSION     DADLSB010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.448"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            82..>469
                     /gene="yiaM"
                     /locus_tag="JN331_004501"
     CDS             82..>469
                     /gene="yiaM"
                     /locus_tag="JN331_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HAY7262381.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTS"
BASE COUNT          118 a          103 c           95 g          153 t
ORIGIN      
        1 ttattgctct aactatatta ataatttagg ttgctgcaca tgcaacttga attatgacag
       61 acatagcctc aaggaatagc tatgaaaaaa atacttgaag caatactggc gattaatctc
      121 gccgtacttt cctgtattgt gtttataaac attattttaa gatatggttt tcagacaagc
      181 attttatctg tagatgaatt gtcacgttat ttatttgtct ggttaacatt tattggcgcg
      241 attgtggctt ttatggataa cgcccacgtt caggtcacct ttctggtgga gaaactctcc
      301 cccgcatggc aacgacgagt cgctctggtt acccattcct taatcttatt tatttgtggc
      361 gcactggcct ggggcgcaac gctaaaaaca attcaggact ggagcgatta ttcaccgatc
      421 ctcggtttgc ccatcggcct gatgtatgcc gcttgtctgc ccaccagcc
//
LOCUS       DADLSB010000442          454 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.449, whole genome shotgun sequence.
ACCESSION   DADLSB010000442 DADLSB010000000
VERSION     DADLSB010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.449"
                     /strain="HUE15"
                     /db_xref="taxon:623"
BASE COUNT          136 a           98 c           68 g          152 t
ORIGIN      
        1 tctgtaatcc tctttgcttc ctgagtaata acttcctgag tgaatattta acctgagctt
       61 gatcctacac atacttatta tgaatgataa aattcattca attaataaca catatattaa
      121 ttgccgttaa aactaaaaac agcatcaata atcaacgcga tataataaac ctgccttaca
      181 tatcaactgc gccagaggta ggattgaaaa cgctctcctg attttccaat tcattttctg
      241 gataaataaa taatttattt ttgtcactat tatttatgta atcatcctgt cagggagagg
      301 gatctcaatt atcaatgctt aattacgtca tcattttgat gacatgaaac cggagcattt
      361 tgcatggaat tttcgttttc acccaaacgt cttgttgttg ctgtcgccgc cgctcttcct
      421 ctcatggcca gcgcagcaga taccccgtca actg
//
LOCUS       DADLSB010000443          438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.450, whole genome shotgun sequence.
ACCESSION   DADLSB010000443 DADLSB010000000
VERSION     DADLSB010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.450"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>438
                     /locus_tag="JN331_004502"
     CDS             <1..>438
                     /locus_tag="JN331_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7262382.1"
                     /translation="IATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHV
                     AIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSR
                     LTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIR"
BASE COUNT           99 a          111 c          139 g           89 t
ORIGIN      
        1 atcgccacaa tggcaacccg gagaggctac cagatgggac gctggcttgc tggcaggctc
       61 atgaaagagc tggggctggt cagctgtcag cagccgactc accggtataa acgtggtggt
      121 catgaacatg ttgctatccc taactacctt gaaaggcagt tcgccgtgac cgagccaaat
      181 caggtgtggt gcggtgatgt gacctatatc tggacgggta agcgctgggc gtacctcgcc
      241 gttgttctcg acctgttcgc aagaaaacca gtgggctggg ccatgtcgtt ctcgccggac
      301 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
      361 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      421 tggcgatacc agatcaga
//
LOCUS       DADLSB010000444          436 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.451, whole genome shotgun sequence.
ACCESSION   DADLSB010000444 DADLSB010000000
VERSION     DADLSB010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.451"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(322..>436)
                     /locus_tag="JN331_004503"
     CDS             complement(322..>436)
                     /locus_tag="JN331_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7262383.1"
                     /translation="YLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          155 a           66 c           71 g          144 t
ORIGIN      
        1 gatgtgacaa catctatttt cattgatttg attcaatata acaatagatt acagaggatt
       61 aacttccagt tacttaaaaa ataaagaaaa aagtgtggaa acaggagaat ttaagtgatg
      121 tgtaaactta tgaatgattt tgtttattta ttttgaagaa cataaaccat tagattttgt
      181 atgggtatgg ttaacttaat gtttattatg ataattaaga atcacactta caataacatc
      241 aattaataaa tggttaatca tttttgatat atcgtaagaa taatgtagtt tttaacacca
      301 tccctggtat ctcaactatc tctataaaac agcatgacgc tgtcgtcctc tggctctatc
      361 ccagatgccg taaaaacgcc ctgcattgct ggcggtatac cagacagtat gacgaatatt
      421 gcgatgccca agataa
//
LOCUS       DADLSB010000445          433 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.452, whole genome shotgun sequence.
ACCESSION   DADLSB010000445 DADLSB010000000
VERSION     DADLSB010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.452"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>433
                     /gene="ipaH9.8"
                     /locus_tag="JN331_004504"
     CDS             <1..>433
                     /gene="ipaH9.8"
                     /locus_tag="JN331_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7262384.1"
                     /translation="LDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADA
                     TESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVR
                     TLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTAN"
BASE COUNT           96 a          121 c          113 g          103 t
ORIGIN      
        1 cttgaccgcc tttccgatac cgtctctgca cgcaatacct ccggattccg tgaacaggtc
       61 gctgcatggc tggaaaaact cagtgcctct gcggagcttc gacagcagtc tttcgctgtt
      121 gctgctgatg ccactgagag ctgtgaggac cgtgtcgcgc tcacatggaa caatctccgg
      181 aaaaccctcc tggtccatca ggcatcagaa ggccttttcg ataatgatac cggcgctctg
      241 ctctccctgg gcagggaaat gttccgcctc gaaattctgg aggacattgc ccgggataaa
      301 gtcagaactc tccattttgt ggatgagata gaagtctacc tggccttcca gaccatgctc
      361 gcagagaaac ttcagctctc cactgccgtg aaggaaatgc gtttctatgg cgtgtcggga
      421 gtgacagcaa atg
//
LOCUS       DADLSB010000446          424 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.453, whole genome shotgun sequence.
ACCESSION   DADLSB010000446 DADLSB010000000
VERSION     DADLSB010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.453"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..205)
                     /locus_tag="JN331_004505"
     CDS             complement(<1..205)
                     /locus_tag="JN331_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262385.1"
                     /translation="MRLWADGRLIFEGDITDAYADYNQAPDIPLILTGQIHFNLRNQP
                     AADFSAKGDVAVADIIRALASTVG"
BASE COUNT          103 a          127 c           94 g          100 t
ORIGIN      
        1 gcccgacagt tgacgccagc gcacggataa tatctgcaac cgcaacatcg ccctttgcgc
       61 tgaaatctgc cgccggctga ttacgcagat taaaatgaat ctgtcctgtc agaatgagcg
      121 gaatatccgg cgcctggttg tagtcggcgt atgcatcagt aatatcacct tcaaaaataa
      181 gccgcccgtc ggcccacagc cgcattttat ttgcggtact ctgctcctgt cgccacacac
      241 cgcgccagct taaattcgtc atatgcgccg gtgacattcc ccagatatac agcgtgattt
      301 gtgtaccagc cacccctcca tatacggcaa tactggcaaa acagcttgcg tcggtaatgg
      361 tcagcatatt gccctggcca ttaccaaacg ttcgtccgtc tgaaagagtg aactccaccg
      421 ttat
//
LOCUS       DADLSB010000447          415 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.454, whole genome shotgun sequence.
ACCESSION   DADLSB010000447 DADLSB010000000
VERSION     DADLSB010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.454"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..394
                     /locus_tag="JN331_004506"
     CDS             <1..394
                     /locus_tag="JN331_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7262386.1"
                     /translation="SSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
                     EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRR
                     LLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          118 a          100 c          115 g           82 t
ORIGIN      
        1 atcgtccatg gaaacgacat tcgtgctgga tgcactggag caggcgttat gggcccgtcg
       61 accgtccggc acggtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac
      121 acagcggctt aaggaagccg gattactggc atcaacagga agtacaggcg actcgtatga
      181 caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
      241 ctggaaaaac cgtgcagaag tggaactggc cacactcacg tgggtggact ggtataacaa
      301 tcgacgattg ctggaaaggc tgggccatac tcctccggca gaagcagaaa aagcttatta
      361 tgcttccatc ggaaacgatg atctggcagc ctgagttcac agataaaaca ctctc
//
LOCUS       DADLSB010000448          411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.455, whole genome shotgun sequence.
ACCESSION   DADLSB010000448 DADLSB010000000
VERSION     DADLSB010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.455"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..>411)
                     /locus_tag="JN331_004507"
     CDS             complement(<1..>411)
                     /locus_tag="JN331_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAY7262387.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEA"
BASE COUNT          116 a          101 c           72 g          122 t
ORIGIN      
        1 gcttcagcta gttgcccctt aagctcttca gccataatct gacccttacc agccatctgt
       61 gataatgctg tcagtgctcg cttaatctct tcttcacttc gccccatcag acgagaatag
      121 ccaatcatat ttctaaaaag ttcttcagaa ccttgaatgc ctagagactc tctagctgaa
      181 gcaccgaaac cagcaaactg tgattgcagt tcagcgatat ctaaacccgt tgtttgagct
      241 aatgactttg tgaaatcttt tacttgattt gcatctttag aaaatacgaa atcaattgaa
      301 cgttcagcac ttgcacgttg aaggccaact tccattactc gctgataagc ggatagtaca
      361 gcctgaaagc tgataagtac acctgctgtt ttaactgctg ctgaacttaa a
//
LOCUS       DADLSB010000449          405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.456, whole genome shotgun sequence.
ACCESSION   DADLSB010000449 DADLSB010000000
VERSION     DADLSB010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.456"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..392)
                     /locus_tag="JN331_004508"
     CDS             complement(<1..392)
                     /locus_tag="JN331_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7262388.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKRCLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSL"
BASE COUNT          107 a           76 c          115 g          107 t
ORIGIN      
        1 agtgaaacat caagagcgac caggtgaggc ggaagagccg ggagcctgct cagtctgtta
       61 tcgctggctt taagtacagt taaggacgga gggagttctg gcagagagcg tagctcattg
      121 ctagagatgt taagttcttg tatgtgcggg ggcaggtatg ggagagagcg taatctgagt
      181 aaacttaaat tgagggctgg ctcttgaaaa gccagacatc ttttcagtaa tcgaactgcc
      241 tgtgttcggt cttctgttgc agcaccttcc ttggcccagt tatcccagat gcggtgataa
      301 tccgtgatat tttgctcttc tacggataaa cgggatatac atcctaaacc acaaaaggag
      361 cggaaaaaag aaggattgtg ggcaggtttc atttattatt gatcc
//
LOCUS       DADLSB010000450          404 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.457, whole genome shotgun sequence.
ACCESSION   DADLSB010000450 DADLSB010000000
VERSION     DADLSB010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.457"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..>404)
                     /locus_tag="JN331_004509"
     CDS             complement(<1..>404)
                     /locus_tag="JN331_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262389.1"
                     /translation="PQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKL
                     LTDAVKMRGLIAPASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLN
                     AQKLRDTQHMMQQILLSLVHALYEGNPQPVFA"
BASE COUNT           93 a           91 c          112 g          108 t
ORIGIN      
        1 ggcaaaaacc ggctgcggat taccttcgta cagcgcatga acaaggctca gcagtatttg
       61 ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc aataacacga aatggctggg
      121 gcgcgtggcc cagtatgcat tgatttgcaa ctccagcata caaaccagat tgcgattaat
      181 ggtctggata ccttcatata tcgatttggg aatacgggtt tctttgctgg cgggcgcaat
      241 cagcccacgc attttcacgg catcggtcag gagtttttgt agatggcttt ccagacgtgg
      301 gcgttcgagt aagttcggtg agaatgcaga ttgatagacc cgattatact cggtcagact
      361 tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt ggcc
//
LOCUS       DADLSB010000451          392 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.458, whole genome shotgun sequence.
ACCESSION   DADLSB010000451 DADLSB010000000
VERSION     DADLSB010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.458"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..359)
                     /locus_tag="JN331_004510"
     CDS             complement(<1..359)
                     /locus_tag="JN331_004510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262390.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVAPP"
BASE COUNT           99 a          109 c           96 g           88 t
ORIGIN      
        1 ggcggggcga ctccgtccgg ccagtgaacc gtgccacact ccgggcagta cataccgccg
       61 gcgctgatac cggcaagaat ggtcgcaaat tcccgctccg tgcagcgggc gatttccgga
      121 tacccttcgt catcaacacg tacaaaccag aagaccagct ttttgtttcc cgcatccaca
      181 aagaacggaa tattcaggtc tgcgcagcat tcaacggcat cgtcaaaact atcaaagcgc
      241 agaacttctg cgtcttcttc gtcaaaaaaa tcatcttcgt gaagcttcac gacatagcgg
      301 ggaagtttgc ttctttgaga ggcgggttta cgtttacggg gtttagctga acgggccata
      361 taaccacctg aaagacaatg acattgcctg tt
//
LOCUS       DADLSB010000452          391 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.459, whole genome shotgun sequence.
ACCESSION   DADLSB010000452 DADLSB010000000
VERSION     DADLSB010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.459"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..278
                     /locus_tag="JN331_004511"
     CDS             <1..278
                     /locus_tag="JN331_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7262391.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESAA
                     IIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          100 a           91 c          104 g           96 t
ORIGIN      
        1 gcctgaacgt gtactgtcgt aatggccagg tggaaatcga caacaacatc ggtgaaaacg
       61 cgctgcgatc ggtggcggtt ggaagaaaaa attatctctt cttcggttct gacaacggtg
      121 gagaaagcgc ggcgattatc tacagtctgc tggtcacctg caaactgaac ggagtggagc
      181 cggaggactg gttgcgcgaa gtgatcgtga agctcaatga ctggccgtcg aaccgagtgc
      241 atgaactgct gccctggaac ttctcagctg taaaataatc tttccgctac gtacttctct
      301 gggcgcttac cactcaacgg catcgtcaaa attatcaaag cgcagaacat agatttatca
      361 tgtgttttat ggatggcttt ctccatcagg c
//
LOCUS       DADLSB010000453          387 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.460, whole genome shotgun sequence.
ACCESSION   DADLSB010000453 DADLSB010000000
VERSION     DADLSB010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.460"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..362)
                     /locus_tag="JN331_004512"
     CDS             complement(<1..362)
                     /locus_tag="JN331_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7262392.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYP"
BASE COUNT          113 a           80 c           68 g          126 t
ORIGIN      
        1 ttgggataca gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc
       61 ggaatacacg ctaatgtcac cataccacga tgataagcat tcatatcacg aatattaaaa
      121 agcgtttcat cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct
      181 tttgttaacg taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc
      241 atctttttta ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg
      301 ttaaatgaac tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc
      361 attattactc cggaaaatgg aagcgac
//
LOCUS       DADLSB010000454          385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.461, whole genome shotgun sequence.
ACCESSION   DADLSB010000454 DADLSB010000000
VERSION     DADLSB010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.461"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>385
                     /locus_tag="JN331_004513"
     CDS             <1..>385
                     /locus_tag="JN331_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7262393.1"
                     /translation="RYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVS
                     YRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSM
                     SPRMTAQLACDALQMALWRRKRPRNV"
BASE COUNT           78 a          108 c          123 g           76 t
ORIGIN      
        1 gcgttacggt gccccacgcc tgacggatga actgcgtgct cagggttacc cctttaacgt
       61 aaaaaccgtg gcggcaagcc tgcgccgtca gggactgagg gcaaaggcct cccggaagtt
      121 cagcccggtc agctaccgcg cacacggcct gcctgtgtca gaaaatctgt tggagcagga
      181 tttttacgcc agtggcccga accagaagtg ggcaggagac atcacgtact tacgtacaga
      241 tgaaggctgg ctgtatctgg cagtggtcat tgacctgtgg tcacgtgccg ttattggctg
      301 gtcaatgtcg ccacgcatga cggcgcaact ggcctgcgat gccctgcaga tggcgctgtg
      361 gcggcgtaag aggccccgga acgtt
//
LOCUS       DADLSB010000455          353 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.462, whole genome shotgun sequence.
ACCESSION   DADLSB010000455 DADLSB010000000
VERSION     DADLSB010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.462"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..347)
                     /locus_tag="JN331_004514"
     CDS             complement(<1..347)
                     /locus_tag="JN331_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262394.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEW"
BASE COUNT           69 a          102 c           86 g           96 t
ORIGIN      
        1 ttccattcaa acgtgaacgg cttcgcctcc tgttctgcgc gccatgcttc aatttcctga
       61 cgcctggcct ctctggccgc ttccagcatt tctggtgtca cagtgaatgg ggctatctca
      121 ccccatttgc cgctttgcag ttcctgccag atttgctgac ccgtcggagc gacatcatca
      181 gcggtggctg tgtaggggac tgcctggtcc ctgtcgtcaa aaaaaacgtc acagtctact
      241 gcgccacttt cggtataacg gggattaatg atttttttaa tttccacggt gcattcctca
      301 cgatgtgcga ataaaaagcc cgggcattgc gccagagaca tgtgcatccg gca
//
LOCUS       DADLSB010000456          352 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.463, whole genome shotgun sequence.
ACCESSION   DADLSB010000456 DADLSB010000000
VERSION     DADLSB010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.463"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            1..352
                     /locus_tag="JN331_004515"
                     /pseudo
     CDS             1..352
                     /locus_tag="JN331_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           82 a           98 c           86 g           86 t
ORIGIN      
        1 aacaggttgt tgatcgcggt tattctgttg ccagcgttgc aacacgtctc gatatcacca
       61 cccacagcct ttatgcctgg ataaagaagt acggtccgga ttcttccact aataaagaac
      121 agtcagatgc tcaggccgag atccgccgtc tccagaaaga gctgaaacgg gttaccgacg
      181 aacgggacat attaaaaaaa gccgcggcgt acttcgcaaa gctgtccgac tgaggtacgc
      241 ctttatccgt gacaactcct gttgctggcc tgttcgcctg ctctgtcggg tgctggatgt
      301 tcatcccagt ggtttttacg cctggcttca gcagccgcat tcacaacgcc at
//
LOCUS       DADLSB010000457          332 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.464, whole genome shotgun sequence.
ACCESSION   DADLSB010000457 DADLSB010000000
VERSION     DADLSB010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.464"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            161..>332
                     /locus_tag="JN331_004516"
     CDS             161..>332
                     /locus_tag="JN331_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7262395.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLS"
BASE COUNT           97 a           56 c           67 g          112 t
ORIGIN      
        1 cgaaaacgat acgctttgtt ggattatatc catgaaactg ctatccgctc tttggatgca
       61 tatcctgtaa actcagaaca tcaggactta aattaaggtt tgatgtattc tctgaataaa
      121 atattaatga tgattttggt aggggcattc gcactaaata atgaaaaaat acaaatccga
      181 gtttattcct gaatttaaga aaaattatct ttcccctgtt tactggttta catggttcgt
      241 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      301 caaaataggg cgttgggtgg gcagattgag ca
//
LOCUS       DADLSB010000458          314 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.465, whole genome shotgun sequence.
ACCESSION   DADLSB010000458 DADLSB010000000
VERSION     DADLSB010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.465"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            24..100
                     /locus_tag="JN331_004517"
     tRNA            24..100
                     /locus_tag="JN331_004517"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="JN331_004518"
     tRNA            143..218
                     /locus_tag="JN331_004518"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           87 a           67 c           78 g           82 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aatt
//
LOCUS       DADLSB010000459          310 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.466, whole genome shotgun sequence.
ACCESSION   DADLSB010000459 DADLSB010000000
VERSION     DADLSB010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.466"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..306
                     /locus_tag="JN331_004519"
     CDS             <1..306
                     /locus_tag="JN331_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HAY7262396.1"
                     /translation="KNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLGYHAENK
                     GLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT           87 a           67 c           72 g           84 t
ORIGIN      
        1 aagaacgggc gcttcggatt tggtcttcgg gccagcaaaa gtcgttcaac aggaagaatt
       61 ctggttctga gtctgctggc aacgttgagc actatcgtaa tgtggttact cggttatcac
      121 gctgaaaata aagggttaca cctgaaatat caggctaaca gcattaaatc ccggcgggtt
      181 atctcatatc tgacgttagc gaagaatgtt cttcgacact cgccacttat tttaagacga
      241 acagtactga gcacagttct aaaccatctg tccagaacct accgaaatat ggtgctggtt
      301 tattagcagt
//
LOCUS       DADLSB010000460          309 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.467, whole genome shotgun sequence.
ACCESSION   DADLSB010000460 DADLSB010000000
VERSION     DADLSB010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.467"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..>309)
                     /locus_tag="JN331_004520"
     CDS             complement(<1..>309)
                     /locus_tag="JN331_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7262397.1"
                     /translation="GGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHE
                     GDSIRFLYAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDCDYDGEHRDE
                     "
BASE COUNT           80 a           87 c           70 g           72 t
ORIGIN      
        1 gttcatcccg atgctcaccg tcgtaatcgc aatcatattc gtacccctcc attcgacgat
       61 acgcaacatc atcaagggta taaaaacctt gtgtcgaata gcggtaaccc attaactgaa
      121 aattggtgcc gaaggtgttc atcgatttcg catagaggaa gcgaatggaa tccccctcat
      181 gacgactgtc gtcggctaac tgactgcgcg catgggtcac atccagcgac actgcccccc
      241 agtttccgag attgcgcccc agtcctaata aaaaggcggt gtaattggca gataattgcg
      301 tcccgccgt
//
LOCUS       DADLSB010000461          305 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.468, whole genome shotgun sequence.
ACCESSION   DADLSB010000461 DADLSB010000000
VERSION     DADLSB010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.468"
                     /strain="HUE15"
                     /db_xref="taxon:623"
BASE COUNT          101 a           61 c           46 g           97 t
ORIGIN      
        1 ccgggggatc aatcatcaat aaatatggcg ttctttttta attggctagc aacataccac
       61 ccctcatttg tatgacaaat gataatcttt catccaaact tcaaatgcaa taactaattt
      121 tatttctttc ggcatcgctc acattttatg gggaaatcga cgatcatttt tggcataaga
      181 aatccattta aaaatggcta catcccttat acctattcag agctacttat tcaggggtta
      241 ttgagatata cactgatggt gtacaaaaaa atcaccgaca ataatcacgt atcaagtaat
      301 agtcg
//
LOCUS       DADLSB010000462          303 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.469, whole genome shotgun sequence.
ACCESSION   DADLSB010000462 DADLSB010000000
VERSION     DADLSB010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.469"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(1..237)
                     /locus_tag="JN331_004521"
                     /pseudo
     CDS             complement(1..237)
                     /locus_tag="JN331_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT          106 a           42 c           45 g          110 t
ORIGIN      
        1 catctataaa aatgtgcatc atctcagatg agtatattga aaaaaatgat gaaggactgg
       61 agatggttta ggcaaatcct gacttctgaa aaagtcatca aacaaaatta agaactctgt
      121 tttatactcg caaaacactt ttactaactc attttgtctt gttttattta ttattacatc
      181 atctactata tttgatttat aaagacaatg tggcgaagtt gagataccat ctgtcattat
      241 cagtccttga agttgatgaa tttttattaa tttaatttat gagtgaatgt gactactatc
      301 aaa
//
LOCUS       DADLSB010000463          301 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.470, whole genome shotgun sequence.
ACCESSION   DADLSB010000463 DADLSB010000000
VERSION     DADLSB010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.470"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..>301)
                     /locus_tag="JN331_004522"
     rRNA            complement(<1..>301)
                     /locus_tag="JN331_004522"
                     /product="23S ribosomal RNA"
BASE COUNT           66 a          108 c           57 g           70 t
ORIGIN      
        1 ctggaaaacc agcctacacg cttaaaccgg gacaaccgtc gcccggccaa catagccttc
       61 tccgtccccc cttcgcagta acaccaagta caggaatatt aacctgtttc ccatcgacta
      121 cgcctttcgg cctcgcctta ggggtcgact caccctgccc cgattaacgt tggacaggaa
      181 cccttggtct tccggcgagc gggcttttca cccgctttat cgttacttat gtcagcattc
      241 gcacttctga tacctccagc atacctcaca gtacaccttc acaggcttac agaacgctcc
      301 c
//
LOCUS       DADLSB010000464          294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.472, whole genome shotgun sequence.
ACCESSION   DADLSB010000464 DADLSB010000000
VERSION     DADLSB010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.472"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..293)
                     /locus_tag="JN331_004523"
     CDS             complement(<1..293)
                     /locus_tag="JN331_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7262398.1"
                     /translation="MTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEGQGENG
                     DIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN"
BASE COUNT           81 a           89 c           60 g           64 t
ORIGIN      
        1 tcattatcac caagaacaat tgaattcccc cccagggcgt tctgaacgcc gatacccagc
       61 gcaccattga cctgcgaacc accgccaaca gacactttat gcgacatgga tatttcaccc
      121 gtccgcaaat taatggtgaa cgggcgcagg ggaccaatat cgccattttc cccctgacct
      181 tcactggtgg gaataaggtg caggcactct tccgaacgac gaaaaatcag gccaaaagct
      241 tcgttgaaaa tcctcagcgc attaacacca cggattttca gctctccggt tatt
//
LOCUS       DADLSB010000465          292 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.473, whole genome shotgun sequence.
ACCESSION   DADLSB010000465 DADLSB010000000
VERSION     DADLSB010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.473"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..>292
                     /locus_tag="JN331_004524"
     CDS             <1..>292
                     /locus_tag="JN331_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262399.1"
                     /translation="DKRDLPTAEEEISRVQASIIKWYASEYYNGRQVLDEIQTPSAIN
                     SELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISV"
BASE COUNT           95 a           55 c           61 g           81 t
ORIGIN      
        1 gataagagag atttaccaac tgctgaagag gaaatatctc gcgtgcaggc gtctattatc
       61 aaatggtatg cgtcagaata ctataatggc aggcaagttc tcgatgagat acaaacgcct
      121 tcagcaatta acagtgagct ttatacaaaa atgatatacc tgaccaggaa ctggtcattg
      181 tatccgaacg gtgatggctg tgtaactata agctcaccag aaataaaaaa taaataccct
      241 gctgccattt gcctggctct gggatttttt ctaagcattg taatttctgt aa
//
LOCUS       DADLSB010000466          291 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.474, whole genome shotgun sequence.
ACCESSION   DADLSB010000466 DADLSB010000000
VERSION     DADLSB010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.474"
                     /strain="HUE15"
                     /db_xref="taxon:623"
BASE COUNT           68 a          102 c           60 g           61 t
ORIGIN      
        1 ggccctgcga ttgtctgaaa acggctcaca actgcaccat tcataatcac atcgcataaa
       61 ccacagaccg gactgactcc ggaaaaacag aggcccgccc ccgggcctcc ccggattcat
      121 ccgtttccct gttcagcctg acagcacgcc cccgcggccg gatgacagac tccgcttcgg
      181 taagcaaagc ggtcttctgt gattccgcca gttgcggctt attcattact caacgtcaaa
      241 cgcccgaatt gaagccaaat catccagacc gctcagctcc tctttcattt c
//
LOCUS       DADLSB010000467          284 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.475, whole genome shotgun sequence.
ACCESSION   DADLSB010000467 DADLSB010000000
VERSION     DADLSB010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.475"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..258
                     /locus_tag="JN331_004525"
     CDS             <1..258
                     /locus_tag="JN331_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY7262400.1"
                     /translation="TVARQINPYIKVDIPETTRGALYSFVYNVGAGNFRTSTLLRKIN
                     QGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
BASE COUNT           75 a           70 c           77 g           62 t
ORIGIN      
        1 actgtcgcca gacaaattaa cccgtacatc aaagtcgata taccggaaac aacgcgcggc
       61 gctctttact cgttcgttta caacgtgggc gctggcaatt tcagaacatc gacgcttctt
      121 cgcaaaataa accagggcga tatcaaaggc gcatgtgacc agctacgtcg ctggacatat
      181 gctggcggta agcaatggaa agggctgatg acccgtcgtg agattgagcg tgaagtctgt
      241 ttgtgggggc agcaatgagc agagtcaccg cgattatctc cgct
//
LOCUS       DADLSB010000468          278 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.476, whole genome shotgun sequence.
ACCESSION   DADLSB010000468 DADLSB010000000
VERSION     DADLSB010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.476"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            36..>278
                     /locus_tag="JN331_004526"
     CDS             36..>278
                     /locus_tag="JN331_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7262401.1"
                     /translation="MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRET
                     YGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKR"
BASE COUNT           82 a           63 c           78 g           55 t
ORIGIN      
        1 tcgaacaatg gcgacaacaa tttcccattg aagcgatgtg tcaggtattt ggtgtatcca
       61 ggagcggtta ttacaactgg gtacagcatg aaccctcaga cagaaaacaa agtgatgagc
      121 ggctaaaact ggagattaag gtggcacata tccgcactcg cgaaacatat ggaacccggc
      181 ggctccagac ggagctggca gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc
      241 ttcgtaagga gctaaggcta cgctgtaagc agaaacgc
//
LOCUS       DADLSB010000469          277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.denovo.477, whole genome shotgun sequence.
ACCESSION   DADLSB010000469 DADLSB010000000
VERSION     DADLSB010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.denovo.477"
                     /strain="HUE15"
                     /db_xref="taxon:623"
BASE COUNT           79 a           65 c           72 g           61 t
ORIGIN      
        1 ccggaagtaa ggtgaaaacg ggcgtcaggc tgccgtgatg gcaatatgcg cccgaaaccg
       61 gccgcatggg taacatgcct gcggccattt ttgtctttat ctcttccaca agtaaatatt
      121 ggcaacccga gctggaagca tcatccagag cactcatgaa acgactgtaa cagctgttga
      181 aaaaccggaa tgcactcttc attgcaaaca tttcagggat gaagtgaaga tgaattgaga
      241 cgtagtatcc acatcaagcc ggcgttatcc ggtgagg
//
LOCUS       DADLSB010000470         3973 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.guided.254, whole genome shotgun sequence.
ACCESSION   DADLSB010000470 DADLSB010000000
VERSION     DADLSB010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.guided.254"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            35..559
                     /locus_tag="JN331_004527"
                     /pseudo
     CDS             35..559
                     /locus_tag="JN331_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            675..1235
                     /locus_tag="JN331_004528"
                     /pseudo
     CDS             675..1235
                     /locus_tag="JN331_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1292..2749)
                     /gene="pepD"
                     /locus_tag="JN331_004529"
     CDS             complement(1292..2749)
                     /gene="pepD"
                     /locus_tag="JN331_004529"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7262402.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3011..3469
                     /gene="gpt"
                     /locus_tag="JN331_004530"
     CDS             3011..3469
                     /gene="gpt"
                     /locus_tag="JN331_004530"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7262403.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3561..>3973
                     /locus_tag="JN331_004531"
     CDS             3561..>3973
                     /locus_tag="JN331_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7262404.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYP"
BASE COUNT          947 a         1005 c         1058 g          963 t
ORIGIN      
        1 tccctcaaag tgatctacta ttccgcgcag ctatttaggg cagtctattt tacagcggca
       61 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc
      121 gcgttttaat cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag
      181 tccggttctg gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg
      241 ggtcttacat cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc
      301 acgcggtgat tactcctggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct
      361 ggcgcatggg gctgggcgca aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa
      421 atacacagcg acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa
      481 acaactcatc ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg
      541 tctgggcagg ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
      601 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
      661 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
      721 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
      781 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
      841 ttcagtggat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
      901 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
      961 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
     1021 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
     1081 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
     1141 cggtgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
     1201 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
     1261 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
     1321 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
     1381 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
     1441 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
     1501 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
     1561 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
     1621 tcaccacgta gtctttaccg ctgtcgatca gtgaacggat caggcagtga atttctacgt
     1681 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
     1741 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
     1801 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
     1861 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
     1921 tcagtgcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
     1981 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
     2041 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
     2101 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
     2161 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
     2221 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
     2281 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
     2341 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
     2401 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
     2461 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
     2521 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggtttacgaa
     2581 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
     2641 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
     2701 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
     2761 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
     2821 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
     2881 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     2941 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     3001 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     3061 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     3121 tggcggtctg gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     3181 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     3241 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     3301 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     3361 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
     3421 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     3481 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
     3541 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     3601 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     3661 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     3721 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
     3781 tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg
     3841 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta
     3901 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
     3961 ttgccgccta tcc
//
LOCUS       DADLSB010000471         2495 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.guided.301, whole genome shotgun sequence.
ACCESSION   DADLSB010000471 DADLSB010000000
VERSION     DADLSB010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.guided.301"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(40..471)
                     /locus_tag="JN331_004532"
                     /pseudo
     CDS             complement(40..471)
                     /locus_tag="JN331_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            635..1192
                     /locus_tag="JN331_004533"
     CDS             635..1192
                     /locus_tag="JN331_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7262405.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            1375..2235
                     /locus_tag="JN331_004534"
     CDS             1375..2235
                     /locus_tag="JN331_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7262406.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          674 a          564 c          634 g          623 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc aggccgggtc gctcattact
       61 gagccatgcg cgggtgtaca gcaggcgctt caggcggaaa gaccagggga aatcaccaaa
      121 ttcatggtaa ccgtaatatt ccttaatcag cgcagtatgc tctctgaggg tggtaacccg
      181 ttctgcgtag cgggtaagga cttccggacg gcgaatatta agctgcaccg cgacaaattg
      241 ctgaacacct ggcagaaccc gggttaagtc tgtcagaaag gtgcccagaa aacgggcggt
      301 ggtgagctga agcgcaattc ccagccggtt atgccttccc cgccgctggt taatgaaggc
      361 aagatcccgc tcatcaagat gaaaatagcg cgccagctgc acgtcattgg gttctgcaac
      421 atagcgacca taattcagtt cctgctcagt ggtcagaaaa tcgacgggca taccggcctc
      481 cctgcctgat ggtcatgaat taacaatttc gcaaccgtcc gaaatattat aaattatcgg
      541 acatcgtaaa actgttacat taatatgtct attaaatcgt aaatttgtaa taatagacat
      601 gagttgtccg atattcgatt taaggtacat ttttatgcga ctttttggtt acgctcgggt
      661 ctcaaccagt cagcagtctc ttgatcttca ggtcagagca ctcaaagacg caggtgtgaa
      721 agcaaaccgt atatttaccg ataaggcatc cggcagttca acagaccggg aagggctgga
      781 tttgctgagg atgaaggtgg aggaaggtga tgtcattctg gttaagaagc tcgaccgtct
      841 tggccgcgac actgccgata tgatccaact gataaaggaa tttgacgctc agggcgtggc
      901 agtccggttc attgatgacg ggatcagtac cgacggtgat atggggcaaa tggtggtcac
      961 catcctgtcg gctgtggcac aggctgaacg ccggaggatc ctagaacgca cgaatgaggg
     1021 ccgacaggaa gcaaagctga aaggaatcaa atttggccgc aggcgtaccg tggacaggaa
     1081 cgtcgtgctg acgcttcatc agaagggcac tggtgcaacg gaaattgctc atcagctcag
     1141 tattgcccgc tccacggttt ataaaattct tgaagacgaa agggcctcgt gatacgccta
     1201 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1261 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1321 ctcatgagac aataaccctg gtaaatgctt caataatatt gaaaaaggaa gagtatgagt
     1381 attcaacatt ttcgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1441 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1501 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1561 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gtgcggtatt atcccgtgtt
     1621 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1681 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1741 gctgccataa ccatgagtga taacactgct gccaacttac ttctgacaac gatcggagga
     1801 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1861 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     1921 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1981 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2041 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2101 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2161 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2221 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2281 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2341 atcccttaac gtgagttttc gttccactga gcgtcagacc cctaaaagaa ctttccgcta
     2401 agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg
     2461 ccaggacggc cagccgggat cgggatactg gtcgt
//
LOCUS       DADLSB010000472         2472 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.guided.302, whole genome shotgun sequence.
ACCESSION   DADLSB010000472 DADLSB010000000
VERSION     DADLSB010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.guided.302"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            26..1159
                     /gene="blaEC"
                     /locus_tag="JN331_004535"
     CDS             26..1159
                     /gene="blaEC"
                     /locus_tag="JN331_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7262407.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1248..1781
                     /locus_tag="JN331_004536"
     CDS             1248..1781
                     /locus_tag="JN331_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7262408.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1778..2095)
                     /locus_tag="JN331_004537"
     CDS             complement(1778..2095)
                     /locus_tag="JN331_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY7262409.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2271..2417)
                     /gene="ecnB"
                     /locus_tag="JN331_004538"
     CDS             complement(2271..2417)
                     /gene="ecnB"
                     /locus_tag="JN331_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7262410.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT          632 a          650 c          612 g          578 t
ORIGIN      
        1 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
       61 cgcctcttgc tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      121 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      181 taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
      241 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      301 cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
      361 taccgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg
      421 cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
      481 aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg
      541 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
      601 tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga
      661 aaagaattac gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt
      721 agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag
      781 caatttaacc ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca
      841 atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg
      901 gccggtcaat cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg
      961 cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac
     1021 gggggcgacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     1081 cgtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctgtcagat
     1141 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     1201 tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc
     1261 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgtagttct cctacgccgc
     1321 cgcgtggcgt gaccgtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg
     1381 agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca
     1441 gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa
     1501 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga
     1561 aagtgtcatt ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat
     1621 accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc
     1681 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     1741 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     1801 caataatccc caatacgatt aacgccagac tcgccagacg catcgggtta gcggactcac
     1861 cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat
     1921 aagccgtccc taccggtaac gatttcatcg cccaggcaag tagcgccata ctgactatca
     1981 tcgccgtcac ggtaataaca ctcggcgtca aacggctaaa gccgtgggta tatttcaggc
     2041 caacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     2101 gctccggaaa tttggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag
     2161 ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt acctttatga
     2221 cgatcgcaga aaaaacaaac ggcacgacac tgctgtcgta ccgttattgc ttattgctgc
     2281 gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca
     2341 cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca
     2401 attgtcttct tcaccataac gtcttccttt taggtaatga accgccccgg gaatcctgga
     2461 gactaaactt cc
//
LOCUS       DADLSB010000473         2251 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.guided.311, whole genome shotgun sequence.
ACCESSION   DADLSB010000473 DADLSB010000000
VERSION     DADLSB010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.guided.311"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            112..915
                     /locus_tag="JN331_004539"
                     /note="aph(3'')-Ib"
     CDS             112..915
                     /locus_tag="JN331_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7262411.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            915..1751
                     /locus_tag="JN331_004540"
     CDS             915..1751
                     /locus_tag="JN331_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7262412.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1723..2136)
                     /locus_tag="JN331_004541"
     CDS             complement(1723..2136)
                     /locus_tag="JN331_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001945043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7262413.1"
                     /translation="MAEQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVA
                     FSCKRRGFCPSCGARRMAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRN
                     PTLRLWPAIFSGEIDVFPGDRRSRLISSMTSVAPA"
BASE COUNT          479 a          625 c          639 g          508 t
ORIGIN      
        1 gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt
       61 cgggatattt ctctatattc gcgcttcatc agaaaactga aggaacctcc attgaatcga
      121 actaatattt tttttggtga atcgcattct gactggttgc ctgtcagagg cggagaatct
      181 ggtgattttg tttttcgacg tggtgacggg catgccttcg cgaaaatcgc acctgcttcc
      241 cgccgcggtg agctcgctgg agagcgtgac cgcctcattt ggctcaaagg tcgaggtgtg
      301 gcttgccccg aggtcatcaa ctggcaggag gaacaggagg gtgcatgctt ggtgataacg
      361 gcaattccgg gagtaccggc ggctgatctg tctggagcgg atttgctcaa agcgtggccg
      421 tcaatggggc agcaacttgg cgctgttcac agcctatcgg ttgatcaatg tccgtttgag
      481 cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg tgtcccgcaa tgccgtcaat
      541 cccgacttct taccggacga ggacaagagt acgccgcagc tcgatctttt ggctcgtgtc
      601 gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg atatggttgt ttgccatggt
      661 gatccctgca tgccgaactt catggtggac cctaaaactc ttcaatgcac gggtctgatc
      721 gaccttgggc ggctcggaac agcagatcgc tatgccgatt tggcactcat gattgctaac
      781 gccgaagaga actgggcagc gccagatgaa gcagagcgcg ccttcgctgt cctattcaat
      841 gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct tctatctgcg attggaccct
      901 ctgacttggg gttgatgttc atgccgcctg tttttcctgc tcattggcac gtttcgcaac
      961 ctgttctcat tgcggacacc ttttccagcc tcgtttggaa agtttcattg ccagacggga
     1021 ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat tgctgatgaa ctgcgcgggg
     1081 ccgactatct ggtatggcgc aatgggaggg gagcagtccg gttgctcggt cgtgagaaca
     1141 atctgatgtt gctcgaatat gccggggagc gaatgctctc tcacatcgtt gccgagcacg
     1201 gcgactacca ggcgaccgaa attgcagcgg aactaatggc gaagctgtat gccgcatctg
     1261 aggaacccct gccttctgcc cttctcccga tccgggatcg ctttgcagct ttgtttcagc
     1321 gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta cgtccacgcg gcgattatag
     1381 ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct acatggcgat ctgcatcatg
     1441 aaaacatcat gttctccagt cgcggctggc tggtgataga tcccgtcggt ctggtcggtg
     1501 aagtgggctt tggcgccgcc aatatgttct acgatccggc tgacagagac gacctttgtc
     1561 tcgatcctag acgcattgca cagatggcgg acgcattctc tcgtgcgctg gacgtcgatc
     1621 cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct ttccgcagct tggaacgcgg
     1681 atggagaaga ggagcaacgc gatctagcta tcgcggccgc gatcaagcag gtgcgacaga
     1741 cgtcatacta gatatcaagc gacttctcct atcccctggg aacacatcaa tctcaccgga
     1801 gaatatcgct ggccaaagcc ttagcgtagg attccgcccc ttcccgcaaa cgaccccaaa
     1861 caggaaacgc agctgaaacg ggaagctcaa cacccactga cgcatgggtt gttcaggcag
     1921 tacttcatca accagcaagg cggcactttc ggccatccgc cgcgccccac agctcgggca
     1981 gaaaccgcga cgcttacagc tgaaagcgac caggtgctcg gcgtggcaag actcgcagcg
     2041 aacccgtaga aagccatgct ccagccgccc gcattggaga aattcttcaa attcccgttg
     2101 cacatagccc ggcaattcct ttccctgctc tgccataagc gcagcgaatg ccgggtaata
     2161 ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg tggctctggt aacgaccagt
     2221 atcccgatcc cggctggccg tcctggccgc c
//
LOCUS       DADLSB010000474         1794 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.guided.329, whole genome shotgun sequence.
ACCESSION   DADLSB010000474 DADLSB010000000
VERSION     DADLSB010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.guided.329"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            <1..312
                     /locus_tag="JN331_004542"
     CDS             <1..312
                     /locus_tag="JN331_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7262414.1"
                     /translation="DGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTL
                     LVQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYE
                     A"
     gene            complement(494..670)
                     /locus_tag="JN331_004543"
     CDS             complement(494..670)
                     /locus_tag="JN331_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY7262415.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(899..976)
                     /locus_tag="JN331_004544"
                     /pseudo
     CDS             complement(899..976)
                     /locus_tag="JN331_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1000..>1794
                     /gene="sul2"
                     /locus_tag="JN331_004545"
     CDS             1000..>1794
                     /gene="sul2"
                     /locus_tag="JN331_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7262416.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAGLE"
BASE COUNT          395 a          486 c          487 g          426 t
ORIGIN      
        1 gacgggaagc agtacgccag cagccgtgac tttgcggcgg caacagggct ggtaccccga
       61 cagtacagca cgggaggtcg gacgacattg ttagggatta gcaagcgggg caacaaaaag
      121 atccgaactt tgttggttca gtgtgccagg gtattcatac aaaaactgga acaccagtct
      181 ggcaagttgg ccgactgggt cagggagttg ttgtgtcgga aaagcaactt tgtcgtcacc
      241 tgtgctctgg caaacaagct ggccagaata gcctgggcac tgacggcgcg acagcaaact
      301 tacgaagcat aaaggcagaa atacaccagt ttaaacaatc attcatctgg ttttgcgaat
      361 actgatattg atgatactaa cggcccaccg gcctgttgag gaacctgtaa aacggaaagg
      421 ctcattgaag ccgtatattt tctggaggtt catcaggcgc ggaactcatc gaggcgcggg
      481 aataaaatcc cattcagacg ccggatagat tcaagcaagc caacttgtcg tcaaaatcgg
      541 tgttgcaaaa acgggagtga ccatagattc cgttttctga ggcgaccccc acatagagac
      601 cttcccggtc attcactttg tagagtttgt ccctgggctt gagattgcgc agcttggtat
      661 cggtcagcat gtaccttgtc cttcttcgtc tccgataccc actctattgt aaacaagaca
      721 tttttatctt ttatattcaa tggcttattt tcctgctaat tggtaatacc atgaaaaata
      781 ccatgctcag aaaaggctta acaatatttt gaaaaattgc ctactgagcg ctgccgcaca
      841 gctccatagg ccgctttcct ggctttgctt ccagatgtat gctattctgc tcctgcagct
      901 aatggagcac cgcaaacagg ttactcgcct ggggattccc tttcgacccg agcatccgta
      961 tgagactcat gctcgattat tattattata gaagccccca tgaataaatc gctcatcatt
     1021 ttcggcatcg tcaacataac ctcggacagt ttctccgatg gaggccggta tctggcgcca
     1081 gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg gggcagatgt gatcgacctc
     1141 ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt
     1201 atcgcgccgg tgctggacgc gctcaaggca gatggcattc ccgtctcgct cgacagttat
     1261 caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc
     1321 ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt
     1381 atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg cacccgctgg cgacatcatg
     1441 gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa
     1501 cgcaaccgcc ttgtccttga tcccggcatg gggttttttc tgggggctgc tcccgaaacc
     1561 tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt
     1621 ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc
     1681 ggggccgcga cactcgctgc agagcttgcc gccgccgcag gtggagctga cttcatccgc
     1741 acacacgagc cgcgcccctt gcgcgacggg ctggcggtat tggcggggct ggaa
//
LOCUS       DADLSB010000475         1601 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.guided.343, whole genome shotgun sequence.
ACCESSION   DADLSB010000475 DADLSB010000000
VERSION     DADLSB010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.guided.343"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..270)
                     /gene="tetR(A)"
                     /locus_tag="JN331_004546"
     CDS             complement(<1..270)
                     /gene="tetR(A)"
                     /locus_tag="JN331_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAY7262417.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQAL"
     gene            376..1575
                     /gene="tet(A)"
                     /locus_tag="JN331_004547"
     CDS             376..1575
                     /gene="tet(A)"
                     /locus_tag="JN331_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAY7262418.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
BASE COUNT          251 a          500 c          494 g          356 t
ORIGIN      
        1 cagcgcctgc ctgaagctgc gggcattccc gatcagaaat gagcgccagt cgtcgtcggc
       61 tctcggcacc gaatgcgtat gattctccgc cagcatggct tcggccagtg cgtcgagcag
      121 cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc tgaaccccca accgttccgc
      181 cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca gggcggcacg
      241 gatcactgta ttcggctgca actttgtcat gcttgacact ttatcactga taaacataat
      301 atgtccacca acttatcagt gataaagaat ccgcgcgttc aatcggacca gcggaggctg
      361 gtccggaggc cagacgtgaa acccaacaga cccctgatcg taattctgag cactgtcgcg
      421 ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc cgggcctcct gcgcgatctg
      481 gttcactcga acgacgtcac cgcccactat ggcattctgc tggcgctgta tgcgttgatg
      541 caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg gcggccggtc
      601 ttgctcgtct cgctggccgg cgctgctgtc gactacgcca tcatggcgac ggcgcctttc
      661 ctttgggttc tctatatcgg gcggatcgtg gccggcatca ccggggcgac tggggcggta
      721 gccggcgctt atattgccga tatcactgat ggcgatgagc gcgcgcggca cttcggcttc
      781 atgagcgcct gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg gctgatgggc
      841 ggtttctccc cccacgctcc gttcttcgcc gcggcagcct tgaacggcct caatttcctg
      901 acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt acgccgggag
      961 gctctcaacc cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt cgccgccctg
     1021 atggcggtct tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt
     1081 ttcggcgagg atcgctttca ctgggacgcg accacgatcg gcatttcgct tgccgcattt
     1141 ggcattctgc attcactcgc ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc
     1201 gaaaggcggg cactcatgct cggaatgatt gccgacggca caggctacat cctgcttgcc
     1261 ttcgcgacac ggggatggat ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc
     1321 ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag
     1381 ctgcaaggct cactggcggc gctcaccagc ctgacctcga tcgtcggacc cctcctcttc
     1441 acggcgatct atgcggcttc tataacaacg tggaacgggt gggcatggat tgcaggcgct
     1501 gccctctact tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa
     1561 cgagccgatc gctgatcgtg gaaacgatag gcctatgcca t
//
LOCUS       DADLSB010000476         1190 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE15
            SAMEA1030091-rid11416433.guided.375, whole genome shotgun sequence.
ACCESSION   DADLSB010000476 DADLSB010000000
VERSION     DADLSB010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030091
            Sequence Read Archive: ERR047440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942130.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 21:18:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,549
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,171
            CDSs (with protein)               :: 4,171
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 142 of 315
            Pseudo Genes (incomplete)         :: 90 of 315
            Pseudo Genes (internal stop)      :: 122 of 315
            Pseudo Genes (multiple problems)  :: 36 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030091-rid11416433.guided.375"
                     /strain="HUE15"
                     /db_xref="taxon:623"
     gene            complement(<1..433)
                     /gene="intI1"
                     /locus_tag="JN331_004548"
     CDS             complement(<1..433)
                     /gene="intI1"
                     /locus_tag="JN331_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7262419.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTG"
     gene            589..1062
                     /locus_tag="JN331_004549"
     CDS             589..1062
                     /locus_tag="JN331_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAY7262420.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          307 a          297 c          314 g          272 t
ORIGIN      
        1 tgcccgttcc atacagaagc tgggcgaaca aacgatgctc gccttccaga aaaccgagga
       61 tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc cgaggtcttc
      121 cgatctcctg aagccagggc agatccgtgc acagcacctt gccgtagaag aacagcaagg
      181 ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca
      241 gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga
      301 tgaaggcacg aacccagtgg acataagcct gttcggttcg taaactgtaa tgcaagtagc
      361 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
      421 tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca
      481 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
      541 cgcagcaggg cagtcgccct aaaacaaagt taacccagga tgagaacctt gaaagtatca
      601 ttgatggctg cgaaagcgaa aaacggcgtg attggttgcg gtccagacat accctggtcc
      661 gcgaaagggg agcagctact ttttaaagca ttgacctaca atcagtggct tctggtgggt
      721 cgcaagacgt ttgaatctat gggcgcactc cccaatagga aatacgcggt cgttacccgc
      781 tcaggttgga catcaaatga tgacaatgta gttgtatttc agtcaatcga agaggccatg
      841 gacaggctag ctgaattcac cggtcacgtt atagtgtctg gtggcggaga aatttaccga
      901 gaaacattac ccatggcctc tacgctccac ttatcgacga tcgacatcga gccagagggg
      961 gatgttttct tcccgagtat tccaaatacc ttcgaagttg tttttgagca acactttact
     1021 tcaaacatta actattgcta tcaaatttgg aaaaagggtt aacaaagcta tgcaatcgac
     1081 ggcaaaaagc ttcgttcgct tcgcgcacta cgccttttcc gcgattgata gcgacgttat
     1141 gtgaatattg ggcactgttg caaatagtcg gtggtgataa acttatcatc
//
